LOCUS       DAZFDP010000001       155845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFDP010000001 DAZFDP010000000
VERSION     DAZFDP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 155845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 155845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..155845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            299..1597
                     /gene="kgtP"
                     /locus_tag="ACHJ1G_000001"
     CDS             299..1597
                     /gene="kgtP"
                     /locus_tag="ACHJ1G_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4761925.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHJ1G_000002"
     CDS             complement(1594..1917)
                     /gene="yfiM"
                     /locus_tag="ACHJ1G_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4761926.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHJ1G_000003"
     CDS             complement(1963..3318)
                     /gene="pssA"
                     /locus_tag="ACHJ1G_000003"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4761927.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHJ1G_000004"
     CDS             complement(3432..6092)
                     /gene="pat"
                     /locus_tag="ACHJ1G_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4761928.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHJ1G_000005"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6124..6822)
                     /gene="tapT"
                     /locus_tag="ACHJ1G_000005"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4761929.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHJ1G_000006"
     CDS             complement(6891..7310)
                     /gene="trxC"
                     /locus_tag="ACHJ1G_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4761930.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHJ1G_000007"
     CDS             7517..8554
                     /gene="yfiF"
                     /locus_tag="ACHJ1G_000007"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4761931.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHJ1G_000008"
     CDS             complement(8602..9291)
                     /gene="ung"
                     /locus_tag="ACHJ1G_000008"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4761932.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9596..9979
                     /gene="grcA"
                     /locus_tag="ACHJ1G_000009"
     CDS             9596..9979
                     /gene="grcA"
                     /locus_tag="ACHJ1G_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4761933.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10035..10622)
                     /gene="eamB"
                     /locus_tag="ACHJ1G_000010"
     CDS             complement(10035..10622)
                     /gene="eamB"
                     /locus_tag="ACHJ1G_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4761934.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHJ1G_000011"
     CDS             10725..11606
                     /gene="yfiE"
                     /locus_tag="ACHJ1G_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4761935.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11815..13149)
                     /gene="srmB"
                     /locus_tag="ACHJ1G_000012"
     CDS             complement(11815..13149)
                     /gene="srmB"
                     /locus_tag="ACHJ1G_000012"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4761936.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13281..14018
                     /gene="trmN"
                     /locus_tag="ACHJ1G_000013"
     CDS             13281..14018
                     /gene="trmN"
                     /locus_tag="ACHJ1G_000013"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4761937.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14003..15625)
                     /gene="nadB"
                     /locus_tag="ACHJ1G_000014"
     CDS             complement(14003..15625)
                     /gene="nadB"
                     /locus_tag="ACHJ1G_000014"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4761938.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15881..16036
                     /gene="rseD"
                     /locus_tag="ACHJ1G_000015"
     CDS             15881..16036
                     /gene="rseD"
                     /locus_tag="ACHJ1G_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4761939.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16033..16608
                     /gene="rpoE"
                     /locus_tag="ACHJ1G_000016"
     CDS             16033..16608
                     /gene="rpoE"
                     /locus_tag="ACHJ1G_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4761940.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16641..17291
                     /gene="rseA"
                     /locus_tag="ACHJ1G_000017"
     CDS             16641..17291
                     /gene="rseA"
                     /locus_tag="ACHJ1G_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4761941.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17291..18247
                     /gene="rseB"
                     /locus_tag="ACHJ1G_000018"
     CDS             17291..18247
                     /gene="rseB"
                     /locus_tag="ACHJ1G_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4761942.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18244..18723
                     /gene="rseC"
                     /locus_tag="ACHJ1G_000019"
     CDS             18244..18723
                     /gene="rseC"
                     /locus_tag="ACHJ1G_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4761943.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18921..20720
                     /gene="lepA"
                     /locus_tag="ACHJ1G_000020"
     CDS             18921..20720
                     /gene="lepA"
                     /locus_tag="ACHJ1G_000020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4761944.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20736..21710
                     /gene="lepB"
                     /locus_tag="ACHJ1G_000021"
     CDS             20736..21710
                     /gene="lepB"
                     /locus_tag="ACHJ1G_000021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4761945.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21983..22663
                     /gene="rnc"
                     /locus_tag="ACHJ1G_000022"
     CDS             21983..22663
                     /gene="rnc"
                     /locus_tag="ACHJ1G_000022"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4761946.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22660..23565
                     /gene="era"
                     /locus_tag="ACHJ1G_000023"
     CDS             22660..23565
                     /gene="era"
                     /locus_tag="ACHJ1G_000023"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4761947.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23577..24305
                     /gene="recO"
                     /locus_tag="ACHJ1G_000024"
     CDS             23577..24305
                     /gene="recO"
                     /locus_tag="ACHJ1G_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4761948.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24317..25048
                     /gene="pdxJ"
                     /locus_tag="ACHJ1G_000025"
     CDS             24317..25048
                     /gene="pdxJ"
                     /locus_tag="ACHJ1G_000025"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4761949.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25048..25428
                     /gene="acpS"
                     /locus_tag="ACHJ1G_000026"
     CDS             25048..25428
                     /gene="acpS"
                     /locus_tag="ACHJ1G_000026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4761950.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25848..25928
                     /gene="shoB"
                     /locus_tag="ACHJ1G_000027"
     CDS             25848..25928
                     /gene="shoB"
                     /locus_tag="ACHJ1G_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4761951.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26122..26382)
                     /gene="yfhL"
                     /locus_tag="ACHJ1G_000028"
     CDS             complement(26122..26382)
                     /gene="yfhL"
                     /locus_tag="ACHJ1G_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4761952.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26438..27286)
                     /gene="yfhH"
                     /locus_tag="ACHJ1G_000029"
     CDS             complement(26438..27286)
                     /gene="yfhH"
                     /locus_tag="ACHJ1G_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4761953.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27495..28130
                     /gene="yfhb"
                     /locus_tag="ACHJ1G_000030"
     CDS             27495..28130
                     /gene="yfhb"
                     /locus_tag="ACHJ1G_000030"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4761954.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28188..28691
                     /gene="tadA"
                     /locus_tag="ACHJ1G_000031"
     CDS             28188..28691
                     /gene="tadA"
                     /locus_tag="ACHJ1G_000031"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4761955.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(28688..30244)
                     /gene="mltF"
                     /locus_tag="ACHJ1G_000032"
     CDS             complement(28688..30244)
                     /gene="mltF"
                     /locus_tag="ACHJ1G_000032"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4761956.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30502..34389
                     /gene="purL"
                     /locus_tag="ACHJ1G_000033"
                     /gene_synonym="purI"
     CDS             30502..34389
                     /gene="purL"
                     /locus_tag="ACHJ1G_000033"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4761957.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34965..36392
                     /gene="qseE"
                     /locus_tag="ACHJ1G_000034"
     CDS             34965..36392
                     /gene="qseE"
                     /locus_tag="ACHJ1G_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4761958.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36557..37270
                     /gene="qseG"
                     /locus_tag="ACHJ1G_000035"
     CDS             36557..37270
                     /gene="qseG"
                     /locus_tag="ACHJ1G_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4761959.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            37260..38594
                     /gene="glrR"
                     /locus_tag="ACHJ1G_000036"
     CDS             37260..38594
                     /gene="glrR"
                     /locus_tag="ACHJ1G_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4761960.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38655..38993
                     /gene="glnB"
                     /locus_tag="ACHJ1G_000037"
     CDS             38655..38993
                     /gene="glnB"
                     /locus_tag="ACHJ1G_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4761961.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(39038..40228)
                     /gene="hmpA"
                     /locus_tag="ACHJ1G_000038"
     CDS             complement(39038..40228)
                     /gene="hmpA"
                     /locus_tag="ACHJ1G_000038"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4761962.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40556..41809
                     /gene="glyA"
                     /locus_tag="ACHJ1G_000039"
     CDS             40556..41809
                     /gene="glyA"
                     /locus_tag="ACHJ1G_000039"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4761963.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41873..43066)
                     /gene="yphH"
                     /locus_tag="ACHJ1G_000040"
     CDS             complement(41873..43066)
                     /gene="yphH"
                     /locus_tag="ACHJ1G_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4761964.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43184..46465
                     /gene="yphG"
                     /locus_tag="ACHJ1G_000041"
     CDS             43184..46465
                     /gene="yphG"
                     /locus_tag="ACHJ1G_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4761965.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            46562..47545
                     /gene="yphF"
                     /locus_tag="ACHJ1G_000042"
     CDS             46562..47545
                     /gene="yphF"
                     /locus_tag="ACHJ1G_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4761966.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            47568..49079
                     /gene="yphE"
                     /locus_tag="ACHJ1G_000043"
     CDS             47568..49079
                     /gene="yphE"
                     /locus_tag="ACHJ1G_000043"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4761967.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            49104..50102
                     /gene="yphD"
                     /locus_tag="ACHJ1G_000044"
     CDS             49104..50102
                     /gene="yphD"
                     /locus_tag="ACHJ1G_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4761968.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            50135..51229
                     /gene="yphC"
                     /locus_tag="ACHJ1G_000045"
     CDS             50135..51229
                     /gene="yphC"
                     /locus_tag="ACHJ1G_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4761969.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            51241..52113
                     /gene="yphB"
                     /locus_tag="ACHJ1G_000046"
     CDS             51241..52113
                     /gene="yphB"
                     /locus_tag="ACHJ1G_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4761970.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(52161..52583)
                     /gene="yphA"
                     /locus_tag="ACHJ1G_000047"
     CDS             complement(52161..52583)
                     /gene="yphA"
                     /locus_tag="ACHJ1G_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4761971.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(52680..53882)
                     /gene="hcaD"
                     /locus_tag="ACHJ1G_000048"
     CDS             complement(52680..53882)
                     /gene="hcaD"
                     /locus_tag="ACHJ1G_000048"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4761972.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGTVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(53892..54704)
                     /gene="hcaB"
                     /locus_tag="ACHJ1G_000049"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(53892..54704)
                     /gene="hcaB"
                     /locus_tag="ACHJ1G_000049"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4761973.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(54701..55021)
                     /gene="hcaC"
                     /locus_tag="ACHJ1G_000050"
     CDS             complement(54701..55021)
                     /gene="hcaC"
                     /locus_tag="ACHJ1G_000050"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4761974.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(55021..55539)
                     /gene="hcaF"
                     /locus_tag="ACHJ1G_000051"
     CDS             complement(55021..55539)
                     /gene="hcaF"
                     /locus_tag="ACHJ1G_000051"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4761975.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(55536..56897)
                     /gene="hcaE"
                     /locus_tag="ACHJ1G_000052"
     CDS             complement(55536..56897)
                     /gene="hcaE"
                     /locus_tag="ACHJ1G_000052"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4761976.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            57033..57923
                     /gene="hcaR"
                     /locus_tag="ACHJ1G_000053"
     CDS             57033..57923
                     /gene="hcaR"
                     /locus_tag="ACHJ1G_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4761977.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            58083..59222
                     /gene="hcaT"
                     /locus_tag="ACHJ1G_000054"
     CDS             58083..59222
                     /gene="hcaT"
                     /locus_tag="ACHJ1G_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4761978.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(59214..60494)
                     /gene="csiE"
                     /locus_tag="ACHJ1G_000055"
     CDS             complement(59214..60494)
                     /gene="csiE"
                     /locus_tag="ACHJ1G_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4761979.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(60685..61539)
                     /gene="yfhR"
                     /locus_tag="ACHJ1G_000056"
     CDS             complement(60685..61539)
                     /gene="yfhR"
                     /locus_tag="ACHJ1G_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4761980.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(61684..62487)
                     /gene="suhB"
                     /locus_tag="ACHJ1G_000057"
     CDS             complement(61684..62487)
                     /gene="suhB"
                     /locus_tag="ACHJ1G_000057"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4761981.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            62606..63343
                     /gene="trmJ"
                     /locus_tag="ACHJ1G_000058"
     CDS             62606..63343
                     /gene="trmJ"
                     /locus_tag="ACHJ1G_000058"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4761982.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            63644..64132
                     /gene="iscR"
                     /locus_tag="ACHJ1G_000059"
     CDS             63644..64132
                     /gene="iscR"
                     /locus_tag="ACHJ1G_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4761983.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            64373..65587
                     /gene="iscS"
                     /locus_tag="ACHJ1G_000060"
     CDS             64373..65587
                     /gene="iscS"
                     /locus_tag="ACHJ1G_000060"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4761984.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            65615..66001
                     /gene="iscU"
                     /locus_tag="ACHJ1G_000061"
     CDS             65615..66001
                     /gene="iscU"
                     /locus_tag="ACHJ1G_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4761985.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            66018..66341
                     /gene="iscA"
                     /locus_tag="ACHJ1G_000062"
     CDS             66018..66341
                     /gene="iscA"
                     /locus_tag="ACHJ1G_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4761986.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            66437..66952
                     /gene="hscB"
                     /locus_tag="ACHJ1G_000063"
     CDS             66437..66952
                     /gene="hscB"
                     /locus_tag="ACHJ1G_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4761987.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            66969..68819
                     /gene="hscA"
                     /locus_tag="ACHJ1G_000064"
     CDS             66969..68819
                     /gene="hscA"
                     /locus_tag="ACHJ1G_000064"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4761988.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            68821..69156
                     /gene="fdx"
                     /locus_tag="ACHJ1G_000065"
     CDS             68821..69156
                     /gene="fdx"
                     /locus_tag="ACHJ1G_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4761989.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            69168..69368
                     /gene="iscX"
                     /locus_tag="ACHJ1G_000066"
     CDS             69168..69368
                     /gene="iscX"
                     /locus_tag="ACHJ1G_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4761990.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            69546..70829
                     /gene="pepB"
                     /locus_tag="ACHJ1G_000067"
     CDS             69546..70829
                     /gene="pepB"
                     /locus_tag="ACHJ1G_000067"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4761991.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            70971..71747
                     /gene="sseB"
                     /locus_tag="ACHJ1G_000068"
     CDS             70971..71747
                     /gene="sseB"
                     /locus_tag="ACHJ1G_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4761992.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(71779..71904)
                     /gene="timP"
                     /locus_tag="ACHJ1G_000069"
     CDS             complement(71779..71904)
                     /gene="timP"
                     /locus_tag="ACHJ1G_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4761993.1"
                     /translation="MKIRCFCIVLLVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(72240..73085)
                     /gene="sseA"
                     /locus_tag="ACHJ1G_000070"
     CDS             complement(72240..73085)
                     /gene="sseA"
                     /locus_tag="ACHJ1G_000070"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4761994.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            73292..78253
                     /gene="yfhM"
                     /locus_tag="ACHJ1G_000071"
     CDS             73292..78253
                     /gene="yfhM"
                     /locus_tag="ACHJ1G_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4761995.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            78254..80566
                     /gene="pbpC"
                     /locus_tag="ACHJ1G_000072"
     CDS             78254..80566
                     /gene="pbpC"
                     /locus_tag="ACHJ1G_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4761996.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            80715..81146
                     /gene="ndk"
                     /locus_tag="ACHJ1G_000073"
     CDS             80715..81146
                     /gene="ndk"
                     /locus_tag="ACHJ1G_000073"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4761997.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            81296..82450
                     /gene="rlmN"
                     /locus_tag="ACHJ1G_000074"
     CDS             81296..82450
                     /gene="rlmN"
                     /locus_tag="ACHJ1G_000074"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4761998.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            82735..83748
                     /gene="rodZ"
                     /locus_tag="ACHJ1G_000075"
     CDS             82735..83748
                     /gene="rodZ"
                     /locus_tag="ACHJ1G_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4761999.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            83775..84893
                     /gene="ispG"
                     /locus_tag="ACHJ1G_000076"
                     /gene_synonym="gcpE"
     CDS             83775..84893
                     /gene="ispG"
                     /locus_tag="ACHJ1G_000076"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4762000.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            85004..86278
                     /gene="hisS"
                     /locus_tag="ACHJ1G_000077"
     CDS             85004..86278
                     /gene="hisS"
                     /locus_tag="ACHJ1G_000077"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4762001.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            86296..86916
                     /gene="yfgM"
                     /locus_tag="ACHJ1G_000078"
     CDS             86296..86916
                     /gene="yfgM"
                     /locus_tag="ACHJ1G_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4762002.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            86927..88105
                     /gene="bamB"
                     /locus_tag="ACHJ1G_000079"
     CDS             86927..88105
                     /gene="bamB"
                     /locus_tag="ACHJ1G_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4762003.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            88223..89695
                     /gene="der"
                     /locus_tag="ACHJ1G_000080"
     CDS             88223..89695
                     /gene="der"
                     /locus_tag="ACHJ1G_000080"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4762004.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            89764..89979
                     /gene="yfgJ"
                     /locus_tag="ACHJ1G_000081"
     CDS             89764..89979
                     /gene="yfgJ"
                     /locus_tag="ACHJ1G_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4762005.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(89976..91346)
                     /gene="xseA"
                     /locus_tag="ACHJ1G_000082"
     CDS             complement(89976..91346)
                     /gene="xseA"
                     /locus_tag="ACHJ1G_000082"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4762006.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            91508..92974
                     /gene="guaB"
                     /locus_tag="ACHJ1G_000083"
     CDS             91508..92974
                     /gene="guaB"
                     /locus_tag="ACHJ1G_000083"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4762007.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            93043..94620
                     /gene="guaA"
                     /locus_tag="ACHJ1G_000084"
     CDS             93043..94620
                     /gene="guaA"
                     /locus_tag="ACHJ1G_000084"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4762008.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(94715..95254)
                     /gene="yfgI"
                     /locus_tag="ACHJ1G_000085"
     CDS             complement(94715..95254)
                     /gene="yfgI"
                     /locus_tag="ACHJ1G_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4762009.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(95270..95788)
                     /gene="yfgH"
                     /locus_tag="ACHJ1G_000086"
     CDS             complement(95270..95788)
                     /gene="yfgH"
                     /locus_tag="ACHJ1G_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4762010.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(96099..96290)
                     /gene="yfgG"
                     /locus_tag="ACHJ1G_000087"
     CDS             complement(96099..96290)
                     /gene="yfgG"
                     /locus_tag="ACHJ1G_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4762011.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            96308..96460
                     /locus_tag="ACHJ1G_000088"
     CDS             96308..96460
                     /locus_tag="ACHJ1G_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762012.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            96642..98885
                     /gene="pdeF"
                     /locus_tag="ACHJ1G_000089"
     CDS             96642..98885
                     /gene="pdeF"
                     /locus_tag="ACHJ1G_000089"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4762013.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(98924..100465)
                     /gene="ppx"
                     /locus_tag="ACHJ1G_000090"
     CDS             complement(98924..100465)
                     /gene="ppx"
                     /locus_tag="ACHJ1G_000090"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4762014.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(100470..102536)
                     /gene="ppk1"
                     /locus_tag="ACHJ1G_000091"
     CDS             complement(100470..102536)
                     /gene="ppk1"
                     /locus_tag="ACHJ1G_000091"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4762015.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(102707..103345)
                     /gene="purN"
                     /locus_tag="ACHJ1G_000092"
     CDS             complement(102707..103345)
                     /gene="purN"
                     /locus_tag="ACHJ1G_000092"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4762016.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(103345..104382)
                     /gene="purM"
                     /locus_tag="ACHJ1G_000093"
     CDS             complement(103345..104382)
                     /gene="purM"
                     /locus_tag="ACHJ1G_000093"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4762017.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            104707..105333
                     /gene="upp"
                     /locus_tag="ACHJ1G_000094"
     CDS             104707..105333
                     /gene="upp"
                     /locus_tag="ACHJ1G_000094"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4762018.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            105419..106708
                     /gene="uraA"
                     /locus_tag="ACHJ1G_000095"
     CDS             105419..106708
                     /gene="uraA"
                     /locus_tag="ACHJ1G_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4762019.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            106803..107504
                     /gene="hda"
                     /locus_tag="ACHJ1G_000096"
     CDS             106803..107504
                     /gene="hda"
                     /locus_tag="ACHJ1G_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4762020.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(107642..108001)
                     /gene="arsC"
                     /locus_tag="ACHJ1G_000097"
     CDS             complement(107642..108001)
                     /gene="arsC"
                     /locus_tag="ACHJ1G_000097"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4762021.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(108022..109485)
                     /gene="bepA"
                     /locus_tag="ACHJ1G_000098"
     CDS             complement(108022..109485)
                     /gene="bepA"
                     /locus_tag="ACHJ1G_000098"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4762022.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            109698..110759
                     /gene="yfgO"
                     /locus_tag="ACHJ1G_000099"
     CDS             109698..110759
                     /gene="yfgO"
                     /locus_tag="ACHJ1G_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4762023.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(110797..111645)
                     /gene="focB"
                     /locus_tag="ACHJ1G_000100"
     CDS             complement(110797..111645)
                     /gene="focB"
                     /locus_tag="ACHJ1G_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4762024.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(111667..113679)
                     /gene="hyfR"
                     /locus_tag="ACHJ1G_000101"
     CDS             complement(111667..113679)
                     /gene="hyfR"
                     /locus_tag="ACHJ1G_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4762025.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(113709..114122)
                     /gene="hyfJ"
                     /locus_tag="ACHJ1G_000102"
     CDS             complement(113709..114122)
                     /gene="hyfJ"
                     /locus_tag="ACHJ1G_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4762026.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(114115..114872)
                     /gene="hyfI"
                     /locus_tag="ACHJ1G_000103"
                     /pseudo
     CDS             complement(114115..114872)
                     /gene="hyfI"
                     /locus_tag="ACHJ1G_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(114869..115414)
                     /gene="hyfH"
                     /locus_tag="ACHJ1G_000104"
     CDS             complement(114869..115414)
                     /gene="hyfH"
                     /locus_tag="ACHJ1G_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4762027.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(115424..117091)
                     /gene="hyfG"
                     /locus_tag="ACHJ1G_000105"
     CDS             complement(115424..117091)
                     /gene="hyfG"
                     /locus_tag="ACHJ1G_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4762028.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(117081..118661)
                     /gene="hyfF"
                     /locus_tag="ACHJ1G_000106"
     CDS             complement(117081..118661)
                     /gene="hyfF"
                     /locus_tag="ACHJ1G_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO4762029.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(118666..119316)
                     /gene="hyfE"
                     /locus_tag="ACHJ1G_000107"
     CDS             complement(118666..119316)
                     /gene="hyfE"
                     /locus_tag="ACHJ1G_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4762030.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(119328..120767)
                     /gene="hyfD"
                     /locus_tag="ACHJ1G_000108"
     CDS             complement(119328..120767)
                     /gene="hyfD"
                     /locus_tag="ACHJ1G_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO4762031.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(120784..121731)
                     /gene="hyfC"
                     /locus_tag="ACHJ1G_000109"
     CDS             complement(120784..121731)
                     /gene="hyfC"
                     /locus_tag="ACHJ1G_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4762032.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(121742..123760)
                     /gene="hyfB"
                     /locus_tag="ACHJ1G_000110"
     CDS             complement(121742..123760)
                     /gene="hyfB"
                     /locus_tag="ACHJ1G_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO4762033.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(123760..124377)
                     /gene="hyfA"
                     /locus_tag="ACHJ1G_000111"
     CDS             complement(123760..124377)
                     /gene="hyfA"
                     /locus_tag="ACHJ1G_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4762034.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(124630..125100)
                     /gene="bcp"
                     /locus_tag="ACHJ1G_000112"
     CDS             complement(124630..125100)
                     /gene="bcp"
                     /locus_tag="ACHJ1G_000112"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4762035.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(125100..125672)
                     /gene="gcvR"
                     /locus_tag="ACHJ1G_000113"
     CDS             complement(125100..125672)
                     /gene="gcvR"
                     /locus_tag="ACHJ1G_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4762036.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            125818..126696
                     /gene="dapA"
                     /locus_tag="ACHJ1G_000114"
     CDS             125818..126696
                     /gene="dapA"
                     /locus_tag="ACHJ1G_000114"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4762037.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            126713..127747
                     /gene="bamC"
                     /locus_tag="ACHJ1G_000115"
     CDS             126713..127747
                     /gene="bamC"
                     /locus_tag="ACHJ1G_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4762038.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            127960..128673
                     /gene="purC"
                     /locus_tag="ACHJ1G_000116"
     CDS             127960..128673
                     /gene="purC"
                     /locus_tag="ACHJ1G_000116"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4762039.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            128827..129054
                     /gene="vapB"
                     /locus_tag="ACHJ1G_000117"
     CDS             128827..129054
                     /gene="vapB"
                     /locus_tag="ACHJ1G_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO4762040.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            129054..129452
                     /locus_tag="ACHJ1G_000118"
     CDS             129054..129452
                     /locus_tag="ACHJ1G_000118"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO4762041.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            129599..130462
                     /gene="ypfJ"
                     /locus_tag="ACHJ1G_000119"
     CDS             129599..130462
                     /gene="ypfJ"
                     /locus_tag="ACHJ1G_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4762042.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            130477..132492
                     /gene="tmcA"
                     /locus_tag="ACHJ1G_000120"
     CDS             130477..132492
                     /gene="tmcA"
                     /locus_tag="ACHJ1G_000120"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4762043.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            132566..133264
                     /gene="ypfH"
                     /locus_tag="ACHJ1G_000121"
     CDS             132566..133264
                     /gene="ypfH"
                     /locus_tag="ACHJ1G_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4762044.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(133374..133574)
                     /gene="ypfN"
                     /locus_tag="ACHJ1G_000122"
     CDS             complement(133374..133574)
                     /gene="ypfN"
                     /locus_tag="ACHJ1G_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4762045.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(133602..134729)
                     /gene="dapE"
                     /locus_tag="ACHJ1G_000123"
     CDS             complement(133602..134729)
                     /gene="dapE"
                     /locus_tag="ACHJ1G_000123"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4762046.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(134733..135089)
                     /gene="yffB"
                     /locus_tag="ACHJ1G_000124"
     CDS             complement(134733..135089)
                     /gene="yffB"
                     /locus_tag="ACHJ1G_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4762047.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            135470..135529
                     /gene="ypfM"
                     /locus_tag="ACHJ1G_000125"
     CDS             135470..135529
                     /gene="ypfM"
                     /locus_tag="ACHJ1G_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4762048.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(135628..138741)
                     /gene="acrD"
                     /locus_tag="ACHJ1G_000126"
     CDS             complement(135628..138741)
                     /gene="acrD"
                     /locus_tag="ACHJ1G_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4762049.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPLVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(138905..140605)
                     /gene="narQ"
                     /locus_tag="ACHJ1G_000127"
     CDS             complement(138905..140605)
                     /gene="narQ"
                     /locus_tag="ACHJ1G_000127"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4762050.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            140811..142790
                     /gene="aegA"
                     /locus_tag="ACHJ1G_000128"
     CDS             140811..142790
                     /gene="aegA"
                     /locus_tag="ACHJ1G_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4762051.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            142858..143433
                     /gene="nudK"
                     /locus_tag="ACHJ1G_000129"
     CDS             142858..143433
                     /gene="nudK"
                     /locus_tag="ACHJ1G_000129"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4762052.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            143559..144602
                     /gene="ypfG"
                     /locus_tag="ACHJ1G_000130"
     CDS             143559..144602
                     /gene="ypfG"
                     /locus_tag="ACHJ1G_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4762053.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(144698..146701)
                     /gene="tkt"
                     /locus_tag="ACHJ1G_000131"
     CDS             complement(144698..146701)
                     /gene="tkt"
                     /locus_tag="ACHJ1G_000131"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4762054.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(146721..147671)
                     /gene="tal"
                     /locus_tag="ACHJ1G_000132"
     CDS             complement(146721..147671)
                     /gene="tal"
                     /locus_tag="ACHJ1G_000132"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4762055.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            147960..150239
                     /gene="maeB"
                     /locus_tag="ACHJ1G_000133"
     CDS             147960..150239
                     /gene="maeB"
                     /locus_tag="ACHJ1G_000133"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4762056.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            150532..150867
                     /gene="eutS"
                     /locus_tag="ACHJ1G_000134"
     CDS             150532..150867
                     /gene="eutS"
                     /locus_tag="ACHJ1G_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4762057.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            150880..151359
                     /gene="eutP"
                     /locus_tag="ACHJ1G_000135"
     CDS             150880..151359
                     /gene="eutP"
                     /locus_tag="ACHJ1G_000135"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4762058.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            151334..152035
                     /gene="eutQ"
                     /locus_tag="ACHJ1G_000136"
     CDS             151334..152035
                     /gene="eutQ"
                     /locus_tag="ACHJ1G_000136"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4762059.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            152032..152835
                     /gene="eutT"
                     /locus_tag="ACHJ1G_000137"
     CDS             152032..152835
                     /gene="eutT"
                     /locus_tag="ACHJ1G_000137"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4762060.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            152832..153848
                     /gene="pta"
                     /locus_tag="ACHJ1G_000138"
     CDS             152832..153848
                     /gene="pta"
                     /locus_tag="ACHJ1G_000138"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4762061.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            153887..154180
                     /gene="eutM"
                     /locus_tag="ACHJ1G_000139"
     CDS             153887..154180
                     /gene="eutM"
                     /locus_tag="ACHJ1G_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4762062.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            154287..154574
                     /gene="eutN"
                     /locus_tag="ACHJ1G_000140"
     CDS             154287..154574
                     /gene="eutN"
                     /locus_tag="ACHJ1G_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO4762063.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            154586..155845
                     /locus_tag="ACHJ1G_000141"
                     /pseudo
     CDS             154586..155845
                     /locus_tag="ACHJ1G_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT        37363 a        39524 c        42048 g        36910 t
ORIGIN      
        1 gtcaggatgg cctttttgcg ttggtgaaaa aaatgcctga tgcgacgctg cgcgtcttat
       61 actcccacat atgccagatt cagcagcgaa tacggcttcc ccaacttgcc cacctccata
      121 cgtgttctcc tcaccagaaa tttatcctta agctcctcaa taaccatttt cctgctaact
      181 aaattcatgg ttaaggttgc ataatgatac gcaacaaatg tataacattt tctttaccaa
      241 aagaaataaa caaaagcgac cgacaaaagc atcggattac ggcaggagac ataatggcat
      301 ggctgaaagt actgtaacgg cagacagcaa actgacaagt agtgatacac gtcgccgcat
      361 ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag tggttcgatt tctatgtcta
      421 ctcgttctgt tcactctact tcgcccacat tttcttccct tccgggaaca cgacgactca
      481 actactacaa acagcaggtg tttttgctgc gggattcctg atgcgcccaa taggcggttg
      541 gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa tcgatgctgt tatcggtgtg
      601 tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca ggttatgaaa ctataggtac
      661 gtgggctccg gcattattgc ttctcgctcg tttatttcag ggattatccg ttggcggaga
      721 atatggcacc agcgccacct atatgagtga agttgccgtt gaagggcgca aaggttttta
      781 cgcatcattt cagtatgtga cgttgatcgg cggacaactg ctagccctac tggttgtcgt
      841 ggttttacaa cacaccatgg aagacgctgc actcagagag tggggatggc gtattccttt
      901 cgcgttagga gctgtgttag ctgttgtggc gttgtggtta cgtcgtcaat tagatgaaac
      961 ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct ctgaaaggat tatggcgcaa
     1021 tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg ggctcccttt gtttctatac
     1081 cttcaccact tatatgcaga agtatctggt aaatactgcg ggaatgcatg ccaacgtggc
     1141 gagtggcatt atgactgccg cattgtttgt attcatgctt attcaaccac tcattggcgc
     1201 gctgtcggat aagattggtc gccgtacctc aatgttatgt ttcggttcgc tggcagccat
     1261 ttttaccgtt cctattctct cagcattgca aaacgtttcc tcgccttatg ccgcttttgg
     1321 tctggtgatg tgtgccctgc tgatagtgag tttttataca tcaatcagtg gaatactgaa
     1381 ggctgagatg ttcccggcac aggttcgcgc attaggcgtt ggcctgtcat atgcggtcgc
     1441 taatgctata tttggtggtt cggcggagta cgtagcgttg tcgctgaaat caataggaat
     1501 ggaaacagcc ttcttctggt atgtgacctt gatggccgtg gtggcgtttc tggtttcttt
     1561 gatgctacat cgcaaaggga aggggatgcg tctttagtga cgggtcagtt gccagacggt
     1621 atagccggtg cttgcaccgg cgacatccca ggccaaatcc ttccagctcc agccgctccc
     1681 ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa ctgacagaga acatcaatcc
     1741 aaacatggca ctgcgatccc ggctcatccc ctgatgctgt gaatattcat ttccggcggc
     1801 ggaaagcatc gccgaggcga taaagtgttg agctttatcc tgcccgctcc agctatcgtt
     1861 agccatatgg ctgcatccag aaagtaacaa tagcgaacag acaaaaagaa tacgcaaaac
     1921 gtggcctcca aaaaacaaac cccgtacagg acggggttgt gattacagga tgcggctaat
     1981 taatcggtcg atgcggatac ggcgcaaacg gcggatgagt ttacgaacct tcaccggata
     2041 atcggcaata ctttgcagat cgcgatagtg cttaacgatg gtggtatgct cgcggatcag
     2101 ctccagttct ttctctcgct gcggcgcaag ctccagttgt ggatcgtgga tcaaaatggc
     2161 gttttccaga tccagacgcc aggcgcgcgg gttcaggtta ttaccggtga tcaacatcca
     2221 cttatcatca acccacatcc cttttaggtg ataggtgttg tcgtcatctt tccataaccg
     2281 aaccactagc tggtcagtat tgacgtaata ctgcaaacgg ctcaggaaac gacgcagatt
     2341 gatctcatag agataaggca atgcgccaat tatcttgaaa ggttcatctt ccggaatgta
     2401 gaagtcgttc gcggttttat caccaacaat aatttcgacc tttttccctt cgcgcagcaa
     2461 ctggataata ttgcgcacaa ggattgctgg caggttgaag tatggcgtac agatggttag
     2521 tttctgctcg gcacaaggca taagatggaa aatggtcttg ttcaacagac tcgatttccc
     2581 cagccccact agcggcgtta cggaaagctg atcgttgtcg gcatcgccct ggaaatgata
     2641 agcggcatca cgcagctcct ggcggaacag acgaatatcg ttcttgattt ccgggctttt
     2701 tggccgatta acatcatcca gacgattaac gccgcggcca ttcataatat tctgtgtaac
     2761 ccattcaaac ataatgtctg acatcttacg gttacggata agatgataac ggtcgtagcg
     2821 atatttatcg tgctgatgca ggtaaacatc gttcaggctg gcaccgctat aaagtacgct
     2881 atcgtcgatg ataaagcctt taaagtgcag aacaccaagg gcttcacggg tattgattgg
     2941 aacgccataa accggaacat ctacgcccgg attttcctgc gccatgcggc agtaccagtc
     3001 agcgttagtg ttagatgccg cagcgccaat gcgtccacgt tgtgcacgat gccagtcgac
     3061 cagcacccgc acatccagtt ccggacgctg ccttttagct tcatacaacg cgttcagaat
     3121 gcctttgcca ccgtcatcct gttcgagata cagggcgaca atgcaaatgc gctgcttcgc
     3181 gctggctatt ttttccagca gcgtctcccg gaagtcggcg ggagcgtaaa agaaatcgac
     3241 atcatcaact gattgagaaa tcttgggtag ttgggcaagg tgttgttgat gtttattacg
     3301 cttaaatttt gacaacatca cagtgcattt cttctctgtt cattgaaggg tcctctgtgc
     3361 aatgcagacg acataagcgg gcaataataa caccagtccc gattaagtgg tcaacatttc
     3421 cagtacctta ctcatgattc ctcgcgttgg gcaagattta gcgtaagccc aacgatcccc
     3481 tcttcgagct ggatatcaac gttaaacccg agcttgcggg ctagcgccac catgccacga
     3541 ttgtttggca tcgtaatacc attcagacgt tgtagtccgt gatctcgcgt ataggtaatc
     3601 aacttttcca ttaagcgtcg acctaagcct aaccctttga gatccgagcg aaccagcaca
     3661 gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac gcgtgacgcc gaggatctct
     3721 tccgtttgat caatacgtcg taccgctaca aacgccattt cccgatcgta gtcgatctgt
     3781 gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct cgctaaagta gcggtaataa
     3841 agatcttctt tggtgactcg cgaaatgaat tgctgaagtt gtggctcatc ttctggcaaa
     3901 atcgggcgga acaagcagcg ttcaccgttt ttcaattcta cccattcttc cagctgatgc
     3961 ggataagggc gcactgccag ccgactctcg ttatcgcctt caaacggcga gatatccagc
     4021 gtgacatcca gcgcggtaaa ttcactgcca gaagccagca aaggatgaat atccagacgc
     4081 tgaatttccg ggcaatcgac aatcaagttg gaaacctgca ccagaagctg gctcaagcct
     4141 gcaacatcca atgggcgtag cgcactgcgt gcacgaatct ttttactttt gatcccctga
     4201 ataaccagat agcgggccag gttcatgttc agcggcggta acgcgacgac ggcttgatct
     4261 tcaggacgcc actccacacc gccttcgccc agcatgatca acggcccgaa aaccggatcg
     4321 tgctcaacca caacccgcaa ctcctgagca ccagcacggt tagccatact ttgcaccaac
     4381 aggccgtgga cccgtgcctg cggccaggcc atttttacgc gatcgaaaat ggcgtttgcc
     4441 gcttgctgga cttcattggc tgtacgcagg tagagcatga cgccctgaac ttccgattta
     4501 tgtggaatat ccggcgaacg caatttcagc gccaccggat aaccaatctg ttcggcaata
     4561 tgcaccgctt cggtgctatc gctggcaatc caggtgggga gcgtgttcat gccatatgct
     4621 tgcaggatag gctgaacttc atgggtatcg agtgacgttg ctccttcggc aatcgcctgt
     4681 tgcaacagaa gatgcgcttc tgcggtattg gaggtcagat tgctgggcaa cgccggcgtt
     4741 tcgcgtagtt gcttctgatt acgccggtac tccaccatat gcataaaagc agtgatggtt
     4801 ccttccgggg tacggtaggt cggcagcccg gcttcgctga ataatcgtcg tgcctcttgc
     4861 gaggaatgtt cgccacacca gttcgtcagc agagaaacat acttgctgcg gggatgatgc
     4921 tttaccgctt caattaatac ttgcgcgctt tctgttgcgg gagcagcggc gctgggcgaa
     4981 tgaataacca tcagcgcatc aaaatcctgg ctgtggagca gaatgtccag cgttttaata
     5041 tagtgctcac tgctggcgtc atcgcgcaga tcgagcgggt tagagattgc cacatgtcct
     5101 ggcagtgcat cgcgcagttt ctggcaggtt tcttcgctta gtgttgccag cttgccattg
     5161 cgtgaccata aggcatcaag cgccagcgcg gcaggcgcag caccgttgct gataatcatc
     5221 aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt ccaccgccga aaacagttcg
     5281 tgggtgtcct gtacccgcaa caaaccggca cgctgaatag ccgcatccca cgccggatcc
     5341 attcctgccg tggtgttcaa cagacgttga gccgccgggc tacgtccgct tttaatcacc
     5401 agaatcggtt tgttgcgaga ggcactacgg gccgccgaaa caaagcgtcg cgcgtcgctt
     5461 aactgttcga gatagagcag gatggcgctg gttttactgt cgcgtgccag atagtcaagc
     5521 aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa taaagtagga aaagcccatc
     5581 ttacgctgtt gcgcccagtc gaggatggtg ttggagacgg cagccgattg cgaaataaac
     5641 gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg cattcagacc ttgccaggga
     5701 gccagtaaac ccagactgtt tggtccaagc aggcgcatgt tatggcgcag ggcgcaggcg
     5761 cggagatctt cgtgttgcga tgccggggcg gaaagaataa tgcaggtttt acagcctttc
     5821 tcgccgagct cttccagaag agcaagatta cggctggcat tggtacataa aaccgcaagg
     5881 tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca acacacccaa caccgctttc
     5941 caggctggcg tcaccgggag taccggtccg ttaaagcctc ccgccagcag gttacgcatc
     6001 atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa ttaccgctat cgattttggt
     6061 cgcagtagtg cttccagtcc tcgctgactc ataccggtct cccgcagaag tgaccgaatg
     6121 attttaaacg ctttctaact gttctgctgt gatgctaccc agatgttgcg tttttcctgc
     6181 cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac cctgctgccg ccccggtatc
     6241 gctggccata tctaacagtg cgatggctac ctcggcagta caatattggc ctgcagcctg
     6301 ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg acggaaatga cgggaagatt
     6361 atccagatac ggacttttac gaaacatctt gcgagcttcc ggccaggtac catcgagcat
     6421 gataaacagc ggtggcttac cggcaggtgg tgtgaagatc acttcccgtt gctcatcagc
     6481 atacgaagcg ggaaagacca ccattggctg atagtccggg ttttgcacca actccagcaa
     6541 atcctgcgag ggttcggtac gcgaccattg aaacgcaacg gtatcaggca aaatatcagc
     6601 aatcagacgc ccggtattac tgggcttcat tggctcggtg tcgaacatca gcaaacagaa
     6661 gcgacttttt gcttgtgctg gggtaattgt cgaacagaga cataatttct ctggcaaaag
     6721 acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga aaaggacgtg ttgcgcgcgc
     6781 aatacgctcg gcgcgtaact ggagaacacc gttttcggtc ataagagagc gtcgaaaaaa
     6841 cgccattgtc gcagaggaga aaacggggca caagatgcgc cccggtaaga ttaaagagat
     6901 tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc cattaagcat gtcgacaacc
     6961 tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa ttccaaagcg actgctcaat
     7021 tcacgttcag cttcggtatt cactttcaca aagcgcactt taccgctacg ctcttgcgcg
     7081 acatcttcaa aaattggtgc gaaattacgg caggggccgc accacggtgc ccagaagtcg
     7141 atcaccacag gtagatcatc cttcagcaat ttgtcgagtg tttcaccggt cgcattaatc
     7201 acctctccgt caaacaagtc gtgaccgcag cgtccgcatt ttgccgcatc ttcgatccga
     7261 tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa cggtattcat aactaacctc
     7321 gggatgagta aggagactta tcggcaacga cgccggtatg tttctaatat gttacatatt
     7381 atcggggaca ttgttccaga caacaatctg ttttattcaa taggtatgat agtcgcaggc
     7441 tttggtacga aatcatggcg aaccgcgcgg acttcgggta atctgcgcgc ttcgcgcagc
     7501 gctggtggag aaaagcatga acgatgaaat gaaaggtaaa agcggcaagg tcaaagtgat
     7561 gtatgtccgc agtgatgatg attctgataa acgtacccac aacccgcgta ccgggaaagg
     7621 gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt cgccccgccc gcgatgacaa
     7681 acagagtcag ccccgtgacc gcaagtggga agattcgccg tggcgcacgg tttcccgcgc
     7741 gccgggtgat gagacgccgg aaaaggccga tcacggtggc atcagtggta aaagttttat
     7801 tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc cgcgtctacg gcgaaaatgc
     7861 atgtcaggca cttttccaga gccgtccgga agcgattgtt cgcgcctggt ttatccagag
     7921 tgtaacgccg cgttttaaag aagccttgcg ctggatggca gcaaaccgca aagcgtacca
     7981 tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg gaacatcacg gcggcgtttg
     8041 cttcttgatc aaaaagcgta acggtacaac cgtgcagcag tgggtaagcc aggcaggcgc
     8101 gcaggattgc gttctggcac tggaaaacga atctaacccg cataacctgg gtggcatgat
     8161 gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg caggatgcgg cactgctgga
     8221 gtcgggggcg gctatccgta ccgcagaagg cggcgcagag cacgttcagc cgattactgg
     8281 cgacaacatt gttaacgtgc tggatgattt ccgtcaggca ggttacaccg tagtgaccac
     8341 ttccagcgag cagggtaaac cgctgttcaa aactagtctg ccagcgaaaa tggtactggt
     8401 gctgggtcag gaatatgaag ggttaccgga tgccgcacgc gatccgaacg atctgcgcgt
     8461 gaagattgac ggtacgggca acgttgccgg gctgaatatc tctgtcgcaa ccggcgtttt
     8521 gcttggtgaa tggtggcgtc agaataaagc ctgataaatc agccgggtgg caactctgcc
     8581 atccggcatt tccccgcaaa tttactcact ctctgccggt aatactggca tccagtcaat
     8641 cggcgtctcg ccacgttgtt ccagccactg atttgccagc acaaaatggt tgcagccaaa
     8701 gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct ttcagtacat gatggcgttg
     8761 cttatctata atcgcccctt tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc
     8821 ttcgcggtgc tggttaatca ggctgatgac tttatcagta aacgtctccc aaccgaggct
     8881 ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca gtattgagca gcagaacgcc
     8941 ctgacgcgcc cagctttcga gatagccatg attagggcgg gtgaagcccg gaatagtatt
     9001 ttccagctct ttatacatat tcaataatga cggaggaatg gcaatgccgg gacgaacgga
     9061 aaatgccaga ccatgcgcct gtcccggtcc gtgataagga tcctggccga gaatcaccac
     9121 tttaacgtca cccaactcgg taaagcggaa cgcgttaaag acatcttttt gtggtggata
     9181 gatagtgacg ccggactgcc gctcgctggc gacggtctga agggtattaa gaaaataagg
     9241 ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat tcgttagcca tctcgctctc
     9301 ctgcgaatct tcaatccgcc tagcttaact gcttctttct acagaacaaa atcatgctca
     9361 ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt aaaatttcac acccgcataa
     9421 gttgatgtaa aacaataaaa tccatttacg accacaatcg atgtgtggtt tttattgatt
     9481 taaatcaaag aatcaagggt ggttgaggag tatatataca ctcaagcaac aatggtttta
     9541 ccaattggcc gcgacaggct gaacaaatca aataattttg ccggggaggc atcacatgat
     9601 tacaggtatc cagattacta aagccgctaa cgacgatctg ctgaactctt tctggctgct
     9661 ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa gcaggttatg cagaagatga
     9721 agtggttgca gtaagcaaac tgggtgacat tgaataccgt gaagttccag tagaagtgaa
     9781 accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt aacgttctgc gtcgtgaaac
     9841 tctggaagat gcagttaagc atccggaaaa atatccgcag ctgaccatcc gtgtatccgg
     9901 ttatgcagtt cgctttaact ctctgactcc ggaacagcag cgcgacgtta tcgctcgtac
     9961 ctttactgaa agcctgtaag gcttaagtag caagttacta agcggaagag ggcgacctct
    10021 tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat agaccagcaa cagggcaagc
    10081 acgatattta actggcgacc atactggcga aacaatcgct gaaacagatg ccccgccagc
    10141 gcccagcaca cattgccaaa cgtcccaatc atcgccagca aaacgctgac gccaactacc
    10201 cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca gtgccgtaac accgtacaaa
    10261 atgattttga cgttcacaaa ctgcaaagca aagctggccc aaaagctgat tggttttgcc
    10321 tgaagtccgt cttcctttgt tgggctggtg gcgattttcc acgccagcca gacaatatat
    10381 gccgcccccg cccaactcaa aaggtgtacc gctgccgggt caatcactgc cagtgaaaat
    10441 gaaatgcccg cacacagtaa catcacaatc aaaaatccca gactcatccc tgccagcacg
    10501 cgggtacttt gacgaaatcc atgcgacgta gcagagctaa gggcgagaat attgttcggt
    10561 cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag cacttaaaag ggtcggtgtc
    10621 acttgttatc tcctctgttt tggtagacag tacccgacac ggaacattta aaaaaatgaa
    10681 tgtttttata ttctagattc gatattttcg ataggtttgg ggttatggat ctgcgccgtt
    10741 ttattacgct taaaaccgtg gtggaagagg gttccttttt gcgagcttcg caaaaattgt
    10801 gctgtacaca atcgacggtg acttttcata ttcagcagct tgagcaggag ttttcagtcc
    10861 agttatttga gaaaattggc cgacggatgt gccttacccg cgaaggaaaa aagttgctgc
    10921 cgcacattta tgaacttacc cgagtgatgg atacgcttcg tgaggctgcc aaaaaggagt
    10981 cagatccgga cggggaacta cgcgttgttt cgggcgaaac gttgctctct taccgaatgc
    11041 cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt gcgtttatcg ttgcagtcgc
    11101 tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggccgatgtc ggcgtctttt
    11161 atcgcgtagg gaatgatgat gccctgaacc gacgagagtt gggtgaacaa tcgctggtgc
    11221 tggtggcttc accgcaaatt gcagatgtcg attttactga gccgggaaga cataacgcca
    11281 gtagctttat tatcaacgaa ccgcaatgtg tcttccggca gatatttgag agcacgctgc
    11341 gtcagcggcg gatcacggtg gaaaacacca ttgagctgtt aagcattgaa agtatcaaac
    11401 gttgtgtcgc ggcgaatatc ggtgtcagtt acttgccccg ctttgcggtg gaaaaagagc
    11461 ttgagagcgg agaattaatt gaattgcctt ttggcgaaca gtcgcagacc attacggcca
    11521 tgtgcgcaca ccatgctgga aaagcggtta gcccggcgat gcacactttt attcagtgtg
    11581 ttgaagagag ttttgtggct ggataaaaat aatgtcggga ttaaacccgg catttaactt
    11641 gtcgacaaag tgcgctttat tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    11701 gatcgtgcaa attcaatata ttgcatgagc cacgtaggcc tgataagcgt agcgcatcag
    11761 gcagttttgc gtttgccagc acttttaata ccaggcttaa acccggcatt gagattactc
    11821 ttctgtcgtt tgcggtggca cgcccgttcc gcttggttta cggcgcttac caatattttt
    11881 ggtgtcgcga tggcgttttt tcacacgcgg tttctctttc tctttagctt ttttcttctc
    11941 ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc tgcttctcgc ttggcgcacg
    12001 cgttttcggg cgtaactcat caataacgcg agctttaatc ggctcttcaa tgtaacggcc
    12061 aaccttgccc agcagcaaat ggtcatgggc ttccaccagc gaaattgcgg tgcctttacg
    12121 accggcgcgc gcggtacgtc cgatacggtg caaataagta tcgccactgc gcggcatatc
    12181 gaagttaaag acgtggctga cgtcaggaat gtcgataccg cgcgcggcaa catcggttgc
    12241 aaccagtacg tttacgcgac cttcggtcaa acgcttgatc gcttcgttac gcttgccttg
    12301 taccatctca ccttcgagat agcagttgtt gatgcccgct tcgcgcagcc agtttgccag
    12361 ctcatgcaca cgttcacgct tacgcacaaa cacaattgag cgggtcgctt ccggctgttt
    12421 taacagatgc accagcaatg cggttttatg ttccaggtca tcggcgcggt aataccactg
    12481 atgaattttt ttgcgctcac gggtggacgg attggcagaa acctccaccg gatcttccag
    12541 cagacgctcg gcaaagtcct gaatcgcatc gccttccagc gttgccgaaa agagcagggt
    12601 ctgtttacgc cagcgcgttt cgccagcaat atgttcgata tcctgagcga agcccatatc
    12661 cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc gcgcggcaat cgaagttctc
    12721 ttcttttatg tattgcagca gacgtccggt cgtggcgacc acgatgtcct gattttcgct
    12781 gaacacttcc gcgtggttca tataggctac gccgccggtg atggtggcga tatccagatg
    12841 cgtatgtttc gccagttcgc gggcatgatc ggccacctgc atcgccagct cgcgagttgg
    12901 ggtgaggatc aaaatacgcg gcggaccgga tttcttacgc gggaaatcga gcaggtgctg
    12961 caacgctggc agcagatacg ccgccgtttt accggtgcct gtcggcgcag aaccgagtac
    13021 atcacggcca tcgagcgcag gcggaatggc ggcagcctga atggcggtcg ggcgagtgaa
    13081 acctttatcc tggagggctt ccagcaggct ttcgtcgagt tcaagttcgg aaaaagtcgt
    13141 tacagtcatg ttctacctct gtgtggggcg ctgattatag acgttacggc tgcaatcttc
    13201 atctgtttgt atggatatcg cttctcgggt attgttctca cccggtgcta tcgccgtttt
    13261 tcctgcaagg ttttattttc atgtcacagt ctacatccgt gcttcgtcgt aatggattta
    13321 cttttaaaca gttttttgtt gctcacgatc gctgtgcgat gaaagtggga acggatggca
    13381 ttttgctggg cgcatgggca ccggtggctg gggtgaaacg ttgccttgat atcggcgcgg
    13441 gtagcgggtt gctggcactg atgctggcgc agcgaaccag tgacagcgta ataattgatg
    13501 ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga aaatatcaac cagtccccgt
    13561 gggcagagcg gattaacgtc catacggcgg atattcagca gtggatcaca cagcagacag
    13621 tacgcttcga tttaatcatc agtaacccac cttactatca gcagggagta gagtgcgcga
    13681 cacctcaacg ggaacaggct cgctatacca ctacgcttga tcacccatcg ctgctgacct
    13741 gtgcggcgga gtgcattacc gaagagggat ttttctgcgt ggtgctgcca gagcagattg
    13801 gtaatggttt tactgaactg gcattaagca tgggctggca tttacgtttg cggacggatg
    13861 tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct ggcattctcc ccgcaggcgg
    13921 gagaatgctt tagcgatcgc ttagtcattc gtgggccaga ccagaactat tccgaagcgt
    13981 atacggcgct gacccaggct ttttatctgt ttatgtaatg attgccgggg gaaaggatcg
    14041 atggaccgga atgcgtgagc agttccgggt aatccagcgt aaaatgcagc ccccggcttt
    14101 ctttgcgcat catcgcgcag cgaacaatca actcggcaac ctgtaccaga ttacgcagct
    14161 ccagcaaatt atttgagacg cggaaatggg cgtaatattc gtctatttct tgttggagca
    14221 tggttatccg ccgcagggcg cgttccaggc gcttcgttgt gcgcacaatg ccaacgtaat
    14281 cccacataaa cagccgtagc tcgtgccagt tatgctgaat tactacccgt tcgtcagggt
    14341 tctcaacgcg gctttcatcc cacggcggta acgtactgac gccgtgggca tcaggcatac
    14401 gtctggtgat atcttctgcc gccgaccagc catagaccag acactccagc aatgaattcg
    14461 aggccatgcg gttagcgccg tgtaagccgg tataactcac ctcgccaatg gcatacaagc
    14521 cctcgacgtc cgtacgccca tgatcatcaa ccattacacc accgcaggta taatgtgcag
    14581 caggcacaat cggtaccggt tcttgtgtga gatcaatccc cagcccgagc aatttttcat
    14641 aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt atggctgatg tcgaggaaca
    14701 tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc gcgggcgaca atatcgcgcg
    14761 gggccagttc gccgcgctca tcaaaatcgg gcataaaacg cgtgccatcc gggcgcttga
    14821 gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa attgcgtgcc tgtgggtgat
    14881 acagtgcggt agggtggaac tgattaaatt cgagattggc aacccgacag cctgcgcgcc
    14941 acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac tgataaacct
    15001 tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc accgtttctt
    15061 tatttcggtt ccatacccac gcgccaacaa cccgtcgcgt gcccgggagg ccaattttgt
    15121 cagaaacaat cagatcaact gcgttgctgc gctccagcac acgaatattc ggatggttct
    15181 gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg gcatgaagaa
    15241 tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg ccattcggtt
    15301 gaacgtgggt atcaaacaaa accccttggt caattaacca ttgcacacag gatcgtgcat
    15361 tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggca atcaatgtgt
    15421 cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata ccgccctggg
    15481 cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc tgatgctggt
    15541 cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accgataatc aacacgtcac
    15601 atgaatgttc tgggagagta ttcattttct ttgtttaatt tactaaacac cgtttggtca
    15661 gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta cagtggctaa
    15721 acacgctgac gcagggcggc gagaaaaaga gaagttactg gctggtggag gattaggtgg
    15781 tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt ctacacagat
    15841 aatgcgatgt tcagattctg tagacttata atgatagata atgatccgtc tacagcatga
    15901 caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa ccctttgctt
    15961 gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg gtttggggag
    16021 actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg ggtccagaag
    16081 ggagatcaga aagcctttaa cctactggta gtgcgctatc agcataaagt ggcgagtctg
    16141 gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc ttttattaaa
    16201 gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg gctgtatcgg
    16261 attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc accttccagt
    16321 gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa agaaatttcg
    16381 aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg aactattgag
    16441 tccctcccgg aagatttgcg catggcaata accttgcggg agctggatgg cctgagctat
    16501 gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg tatcttccga
    16561 gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg atagcgggat
    16621 actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg gatggcgaaa
    16681 cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag aaaacctggg
    16741 aaagctatca cttaatccgt gactcaatgc ggggtgatac tcccgaggtg ctccatttcg
    16801 atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa ccggcgacat
    16861 tgatcccgga agcccagcct gcgccgcatc aatggcagaa aatgccattc tggcagaaag
    16921 tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc agcatgcgta tcgcttgcag
    16981 ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag cccgaaacgc
    17041 cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg ggagtacctt
    17101 ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt cgtcgcatta
    17161 atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag cttcagtttg
    17221 agcaggcgca aacccagcaa gccgctgtac aggtgccagg aattcaaact ttaggaacgc
    17281 aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta gcctgttatt
    17341 ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga tgaacctggc
    17401 cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg gtgttgagtc
    17461 tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt tgcaaatgga
    17521 tggcccgcgc cgggaagtgg tacagcgtgg caatgaaatc agctattttg aaccgggact
    17581 tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc tcatctatac
    17641 cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca cgcgtattgc
    17701 tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct acagctacat
    17761 cgtgtggatg gacatcgaat cgaaattacc gatgagggtt gatctccttg atcgcgatgg
    17821 tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc aatcaggata tcagtagcag
    17881 tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg taggtgaaaa
    17941 agctaaattc agctggacgc caacctggtt gccacagggt tttagcgaag tttccagtag
    18001 tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct attccgacgg
    18061 attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg atcagatgtt
    18121 gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa tcaccattgt
    18181 cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt tcggggcagc
    18241 gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc gctggtcagt
    18301 tgtgatgtta aagtttcatg cagcagctgc gcttcacgcg ccggttgcgg tagccgcgtg
    18361 ttaaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga tgagccgtta
    18421 gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag ctccgcatta
    18481 ctggtttata tgtcgccgct ggtgggatta ttcctcatcg cttcgctatt tcagctactc
    18541 tttgcttctg acgtcgcagc attatgtggt gcggtcctcg gcggtattgg cgggttcctg
    18601 attgcgcgcg gctactcgcg taagtttgct gcccgggcag aatggcaacc gatcatctta
    18661 agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat cttcggaaga cgcgagccag
    18721 tgatttcact ggctttgttg cattttgcat aacaaatgcg aggacgtttc cagataccac
    18781 aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg cggcgtttct
    18841 attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa ccgggcatta
    18901 aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata gctcacattg
    18961 accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc ctgtctgacc
    19021 gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt ggcataacca
    19081 tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc tatcagctga
    19141 actttatcga caccccgggc cacgttgact tctcctatga agtttcccgt tcgctggctg
    19201 cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtagaagcg caaaccctgg
    19261 caaactgcta caccgccatg gaaatggatc tcgaagtggt gccggtactg aacaagattg
    19321 acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc gtcggcatcg
    19381 acgccaccga cgcggtgcgc tgttcagcga aaaccggcgt tggcgtgcag gacgttctcg
    19441 aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc ggaaggcccg ttgcaggcac
    19501 taattatcga ctcatggttc gacaactacc tgggcgttgt ttcacttatc cgtattaaaa
    19561 acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag acctataacg
    19621 ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg aaatgtggcg
    19681 aagtaggctg gctggtatgt gcgattaaag atatccacgg cgctccagtc ggcgatacct
    19741 taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa gtcaaaccgc
    19801 aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc cgtgacgcgc
    19861 tgggtaaact cagcctgaac gatgcttcac tgttctatga gccggaaagc tccagcgcgc
    19921 tgggctttgg tttccgctgc ggcttcctcg gcctgctgca catggagatc atccaggaac
    19981 gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta gtgtatgaag
    20041 ttgaaaccac gtcaagagaa gttatttacg tcgacagtcc atccaagctg ccagcggtaa
    20101 ataacatcta cgaactgcgc gagccgattg ccgaatgtca catgctgctg ccgcaggcat
    20161 atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc aatatggttt
    20221 accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg gtgctcgact
    20281 tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac aacttcaaac
    20341 gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa cgtgttgatg
    20401 cgttggcgct gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag ttggtggaga
    20461 agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca gcgattggta
    20521 cgcacattat tgcgcgatcc accgtgaaac agctacgtaa aaacgtactg gctaaatgtt
    20581 atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa ggtaagaaac
    20641 gcatgaagca gatcggtaac gtcgagctgc cgcaggaagc gttcctcgcc attctgcacg
    20701 ttggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt gccctgattc
    20761 tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc tttttcgcac
    20821 ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca ctggataaag
    20881 caaccctgaa aaaggttgcg ccgaagcctg gctggctgga aaccggtgct tctgtttttc
    20941 cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc cagatcccgt
    21001 caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag aagtttgctt
    21061 atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat ccgaaacgcg
    21121 gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc aagcgcgcgg
    21181 tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg acgattcaac
    21241 cgggatgcag ttccggccag gcgtgtgaaa acgcgctacc ggtcacctac tccaacgtgg
    21301 aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg accagcggat
    21361 tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc gagcgtaaag
    21421 agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag gaccaggtgg
    21481 ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg ccgggacaat
    21541 acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg ggttttgtgc
    21601 ctgaagcgaa tctggtcggt cgggcaacgg ctatctggat gagtttcgat aagcaagaag
    21661 gcgaatggcc gacaggtgtg cgcttaagtc gcattggcgg catccattaa tagcccatct
    21721 tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata aatctcccgt
    21781 ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta aggttggcac
    21841 ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta aactgaaact
    21901 gcagcgaagc agttagcaga gccatgtata tcaggtctgt ttcgtgtgct gaattgttga
    21961 cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc aacggaagct
    22021 gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc gtagtgccag
    22081 cagcaaacat aacgagcgtt tagaattttt aggcgactct attctgagct acgttatcgc
    22141 caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc ggatgcgcgc
    22201 cacgctggtc cgtggcaata cgctggcgga actggcgcgc gaatttgagt taggcgagtg
    22261 cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt caattctcgc
    22321 cgacaccgtc gaagcattaa ttggtggtgt attcctcgac agtgatattc aaaccgtcga
    22381 gaaattaatc ctcaactggt atcaaactcg tttggacgaa attagcccag gcgataaaca
    22441 aaaagatccg aaaacgcgct tgcaagaata tttgcagggt cgccatctgc cgctgccgac
    22501 ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta tccactgcca
    22561 ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta aggctgagca
    22621 ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga taaaagttac
    22681 tgcggattta ttgccatcgt cggacgtccg aacgttggca aatccacatt gttgaacaaa
    22741 ctgctggggc agaagatctc catcacctcc cgcaaagcgc aaacaactcg tcaccgcatt
    22801 gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc tggcctgcat
    22861 atggaagaaa aacgcgctat taaccgcctg atgaacaaag cggcgagcag ctctattggc
    22921 gacgttgagc tggtgatttt tgtcgttgaa ggcacccgct ggacgccgga cgacgaaatg
    22981 gtgctcaaca aactgcgcga tggcaaagcg ccggtgatcc tcgcggtgaa caaagtggat
    23041 aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag tcagatgaac
    23101 ttcctcgata tcgtgccgat ctctgctgaa accgggctga atgtcgacac tatcgcagca
    23161 atcgtgcgta agcatctccc tgaagcgacc catcacttcc cggaagatta catcaccgat
    23221 cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg tttcctcggc
    23281 gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa cgaacgcggt
    23341 ggttatgaca tcaatggttt gattctcgtt gagcgtgaag ggcagaagaa gatggttatt
    23401 ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga catgcaggaa
    23461 atgttcgaag cgcctgtcca ccttgaactg tgggtaaaag tgaaatccgg ttgggccgac
    23521 gacgaacgcg cactgcgcag tctcggttac gttgacgatc tttaagagtc actccgatgg
    23581 aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc agcctgatgc
    23641 tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc gcacgctcta
    23701 aacgctctac tctgaaaggt gcattacagc ctttcacccc tctcttgcta cgtttcggcg
    23761 ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagctgt ctcgctggcg ctgccattaa
    23821 gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc gtactggaat
    23881 acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag tctcttgcag
    23941 gtgccactgg tacgccagaa cccgcgctgc gccgctttga actggcactg ctcgggcatc
    24001 tgggctatgg cgtcaatttt acccattgtg cgggtagcgg cgagccggta gatgacacca
    24061 tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc gacaataaaa
    24121 cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac gcagacacac
    24181 tacgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc ggtaaaccat
    24241 taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa acacattatg
    24301 aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc atattgcaac
    24361 gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt ttattgctga
    24421 gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc acattactga
    24481 ccgtgatgtg cgcatcctgc gtcagacgct ggatacccgc atgaatctgg agatggcggt
    24541 gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct gtctggtacc
    24601 ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gtcgcagggc agcgtgacaa
    24661 aatgcgcgat gcctgcaaac gtctggcaga tgccgggatc caggtttctc tctttatcga
    24721 cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta tcgagatcca
    24781 caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc tggcgcgtat
    24841 cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgctg gtcacggtct
    24901 gacctatcac aacgtgaaag ctattgccgc catccctgag atgcatgaac tgaatatcgg
    24961 tcatgccatt attggtcgtg cagtgatgac cggactgaaa gatgcggtgg cagaaatgaa
    25021 gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggcac ggatatcgta
    25081 gagatcgctc gcatagaagc agtgatcgcc cgatccggtg agcgcctggc acgccgcgta
    25141 ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg ttttctggcg
    25201 aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg gcaccgggat ccgcaatggt
    25261 ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg gctacggcta
    25321 tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca tgtaacgctg
    25381 gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa taataccgtt
    25441 ctctttaaaa agtaaaaccc ccgaactggt aaggaacggg ggttttactt tttaacttta
    25501 attttgtgca gcatgcaccc tcaacaacag cgattatgga tcagtgtact tatcttttac
    25561 aagcaacttg caatctttag cataaaaact cgagccttta cgaagaaagc aatattgatg
    25621 gaaagattaa cgtgaccgcc aattcgtaag tacattaaaa ttggcttcgt tattgaagat
    25681 tttgctgtgc tttacaccat gccacagaat tcccccattg aaacgagtgg tgtcgtcaaa
    25741 gctctggtgt ggagtgcagc atgcaccctc aataactcgc acgttcagtt ttggggagat
    25801 gtaagggcta atctgaacgg ctgcattcct tgtttaagga aaaacgaatg actgattgcc
    25861 gatacctgat taaacgggtc atcaaaatca tcattgctgt tttacagctg atccttctgt
    25921 tcttataaca caaggaaacg tacttaaggt gcgtccggtg aaccaatcgg acgcgccttt
    25981 aataactata aataagtgtc ttggcagata ctatataaat taacttagtg aatgattatg
    26041 ctaatgtcat caattaaata aatataatgg cgttaaggct tcccagtaat ataattaata
    26101 ctctacttcc agagtagaat attaaatttt atccgcgtgg tgcatcagca caaatttatc
    26161 ccacaactgt tcttctgtct cgacatgcgc cggatctttc acaatagtat tggggatcgg
    26221 gcacaccttc tggcaggttg gtgtctcgta gtgccctacg cattcggtac acttatcgct
    26281 gttaatctcg tagatatgat ctcccattga aatcgcctca ttcgggcatt cgggttcaca
    26341 catatcacaa ttgatgcatt ttttagtgat taacaacgcc ataaggaaac tctcaaaaca
    26401 acacaaaccg ggcgggcatt atacgcgcat tctttactca gaccagtttt ttcaccagcg
    26461 cttcactatg acgaatacgt tctggtgcca gttccagatc ctgctgaatc agcgcaatga
    26521 acagcaaatc cgttaacatt ccctgagcgt gacaagcaga gattgaagcg ctgtttgtcg
    26581 cttgttcttc ggcaatggta tacaggcaat gagaagcacg ctgttgcagg gcattcggag
    26641 taaagccggt aatcgccagc acttttccgc ccactcgcag catctcatct gccgccaggt
    26701 ttaactcgcg tcgtacaccg gtgtaggaaa tggctaataa cagatcgtca ggggacgacg
    26761 cctgtactgt tgcgagtagc gcatgcatat cgcgcactgc ggcagcattg aagccaatct
    26821 tcatcagctt ccaggcaaag ttttgcgcca ccagacccga agcgccaata ccggtcagaa
    26881 ttatccgccg cgcagagcgc aacattgtta cgcattcatg cagtttctct tcactattaa
    26941 cgtttagcgt tgcgtacata gcggcagtat tttctttaat cagtttttcg ccgaccagcc
    27001 gtaacggatc atcaccgcgg atttggttat gaatgggcac ggagggcgat tccggctggc
    27061 ttgccagcgc ttcactcaac gccaacttaa gcgccggaaa acctttatag ccgagttttt
    27121 gcgcgaactt cacgacgctg gactgactga ctccggcttc gttggccagt tgctgagagc
    27181 ttaaatggcg cgccgtatca ggttgtagca gcagataatc cgccagtttt ttatcactct
    27241 gggcaagccc ctggtaacgc tgacggatac gaagtaagcc gttcatatct ttcctcgcga
    27301 tgaaatagtg gcgacaacag gcaatatgag agacacgcga gtgaatttta tattccattg
    27361 ccgctaattg ccatgaatta aaaattccag ttagccatga tgcggtcgat tgttgccgga
    27421 aaagtgatga tctatctgtt aggctatgta gtagcgaaaa ttaatcagga tgtttcagtc
    27481 cagaggagta tggtttggca actcacgagc gtcgtgtggt gttttttgac ttagatggaa
    27541 cattgcatca gcaggatatg ttcggcagtt ttctgcgcta tttactgcgt cgccaaccgc
    27601 tgaatgcgtt acttgtcctg ccgttgttac cgattatagc cattgcgtta ttgataaaag
    27661 gtcgtgcggc acgctggccg atgagtctgc ttctgtgggg gtgcactttt ggtcacagcg
    27721 aagcacgttt acagacgttg caggccgatt tcgtgcgctg gtttcgcgac aatgttaccg
    27781 cctttccgct ggttcaggag cgattaacca cctacctgtt aagttccgat gctgatatct
    27841 ggttgattac cggctctccg cagccgctgg ttgaagcggt ttatttcgat acgccctggc
    27901 tgccgcgggt taatcttatc gccagccaaa ttcagcgtgg ctatggtggt tgggtattga
    27961 cgatgcgttg tctgggacat gaaaaggtcg cacaactgga gcgcaaaatc ggcactccgc
    28021 tgcggctgta cagtggctat agcgacagta atcaggacaa tccgctgctt tatttctgtc
    28081 agcatcgttg gcgagtaacc ccgcgcggtg aactccagca actggaatag agtaaagcat
    28141 agcgtccgtg tataatgcgc cgcgctttta taaccggagt tttctttttg tctgaagtcg
    28201 aatttagcca cgaatactgg atgcgtcacg cgctgacgct ggcgaaacgt gcctgggatg
    28261 agcgggaagt gccggtcggc gcggtattag tgcataacaa tcgggtaatc ggcgaaggct
    28321 ggaaccgccc gattggtcgc catgatccca ccgcacatgc agaaatcatg gccctgcggc
    28381 agggtggtct ggtgatgcaa aattatcgtc tgatcgacgc cacgttgtat gtcacgcttg
    28441 aaccatgtgt aatgtgtgcc ggagcgatga tccacagtcg cattggtcgc gtggtctttg
    28501 gtgcgcgtga cgcgaaaact ggcgctgcgg gatctttaat ggatgtgctg catcatccgg
    28561 gtatgaatca ccgagtggaa attacggaag gaatactggc ggatgagtgc gcggcgttgc
    28621 tcagtgactt ctttcgcatg cgccgccagg aaattaaagc gcagaaaaaa gcgcaatcct
    28681 cgacggatta attttgtttc tcttcactcc ctttcctgga aaacagcaga gagggagaat
    28741 gggtcaaata gtttgatgac gactgggaaa gaaacgagag aaaaggaaat ttctctttgc
    28801 ccaactccgt aggcgatacc gccggataat cctgcgccag ttgcatcgcc gcttctgtag
    28861 cctgcttctc tttctcttgc agataaccca ccaggctaat ctgatactta cgaatatttt
    28921 cgacataagc gtaggcttca tgtccacgag cgtagccgta agtcagcttg ctgtaatagg
    28981 gtttctggct aagtaaaggc agacgctgtt ttacgtcagc ccaactgtca ggattccctt
    29041 tggtttttgc cgtcagggcg cgggcatcca gcatatgcgc atagcccata ttgtacgcag
    29101 cgagggcaaa ccagatccgc tcgttctccg gcacactttc cggcacttta ctcatcatat
    29161 cctgcaaata acgcacgcca ccgctgatgc tctgttcggc atcggtacga tccgtaatgc
    29221 cgaggctttg cgcggtattt ttggttaaca tcatcatgcc gcgcacaccc gtcggtgaag
    29281 tggcctgtgc atcccagtgc gattcctgat aagcaatagc ggccagcaaa cgccagtcaa
    29341 tttcttcggc gtatttctca aacaggggct ttaactgcgg cagtaccgca tcgacggcgc
    29401 gtaaaaatgt gcgcgtatcg acgtaatcaa aatcatcgcc atgccccagg tatttctctt
    29461 caatgcgtgc cagcgtaccg tcttcattca tttcgttgaa gaagtcgagc agggcggcgg
    29521 aaagggtatt atcgccatct aacgggctaa accaagtcac cggttgttca tcggtgatat
    29581 cgagcgctac ggcgagctcc gggtgaacgc gctgaaacag gctgatggcg acagaatcag
    29641 caatggtgta atcgagtttt ccttcgatga catcttccat taattccgca gagccttttt
    29701 tgtcgtctac cttccagctt aattccggga attttgtttc tttcagggtc tggagatcgt
    29761 taaccaccac atgacccggt gcaacggtga gttgctccgc cgtcaggttg cccagcgtac
    29821 gtgggcgata ctgacccact ttataaacca gttgttgtga cacggaataa taggtagggc
    29881 caggctgata attttttacc cgctcactgt tatagaccag tcctgccgcc agcaggtcgg
    29941 cgttaccatt atcaaggtcg tcaaacagct ggctgatatt ctgccgcacg gtcactttca
    30001 gttttacgcc gaggtaatcg gcaaactgtt tcgccagttc gtaatccagg ccaaaaggtt
    30061 tcccgttgat ttcgttataa gtcaggggag tatgaatggt gctcacacgc aactctcccc
    30121 gcgcttgaat ggcggcgata cggttgtcgg ctttaccaaa ccagggaatg gatggccaga
    30181 gagcgaccgc gagcagcagt gccagaatgc cgatgaacag ataattaatc tttaattttt
    30241 tcaattagtt aattctctgt gtcgtgcgcg tcccagcttg aaaaaacgta ataatagtga
    30301 aaggtttact cttaaatgag cggcattttg cgtaaacctg cgccagatgg caacttatta
    30361 cagccattgg cggcacgcgt tgctaattca cgatggtgat tttatttcca cgcaaacggt
    30421 ttcgtcagcg catcagattc tttataatga cgcccgtttc ccccccttgg gtacaccgaa
    30481 agcttagaag acgagagact tatgatggaa attctgcgtg gttcgcctgc actgtcggca
    30541 ttccgaatca acaaactgct ggcacgtttt caggctgcca ggctcccggt tcacaatatt
    30601 tacgccgagt atgtccattt tgctgacctc aatgcgccgt taaacgatga tgagcacgca
    30661 caacttgaac gcctgctgaa atatggcccg gcactcgcca gccacgcccc gcaaggcaaa
    30721 ctcctgctgg tgaccccgcg tcctggcacc atctctccct ggtcttcgaa agcgaccgat
    30781 attgcccata actgcgggct acaacaggta aaccgccttg agcgcggcgt tgcttactat
    30841 atagaagccg gtacgctgac caatgaacaa tggcagcagg ttaccgctga actgcacgac
    30901 cgcatgatgg aaacggtctt ttttgcttta gatgatgcag agcagctgtt tgcacaccat
    30961 caaccgactc cggtcaccag cgttgatttg ctggggcagg gccgtcaggc gctaatcgac
    31021 gctaacctgc gtcttggcct ggcactggcg gaagatgaaa ttgactatct gcaggatgct
    31081 ttcacaaagc ttggtcgtaa cccgaacgac atcgagctgt atatgttcgc ccaggcgaac
    31141 tccgagcact gccgccacaa gatttttaac gccgattggg ttatcgacgg tgagcaacag
    31201 ccgaaatcgc tgtttaagat gatcaaaaac accttcgaaa ccacgccaga ttacgttctc
    31261 tctgcttata aagataacgc cgccgtaatg gaaggttctg aagtgggccg ctactttgct
    31321 gaccacgaaa cgggccgcta cgatttccat caggaaccgg cgcatattct gatgaaagtc
    31381 gaaactcaca accacccgac ggcgatttct ccgtggccgg gcgcggcgac cggttccggc
    31441 ggtgaaatcc gcgatgaagg tgccaccggc ctcggcgcga agccgaaagc gggtttggtt
    31501 ggtttctccg tttccaacct gcgaattcct ggcttcgaac agccgtggga agaagatttc
    31561 ggtaagcctg agcgcattgt caccgcgctg gacatcatga ccgaaggccc gctgggcggc
    31621 gcggcgttta acaacgaatt tggtcgtccg gcactgaatg gctacttccg tacttatgaa
    31681 gaaaaagtga acagccacaa cggcgaagag ctgcgcggtt atcacaaacc gatcatgctg
    31741 gcgggcggga tcggcaacat tcgcgccgat cacgtacaaa aaggcgagat caacgtcggt
    31801 gcgaagctgg tcgttctcgg cggcccggca atgaacatcg gtcttggcgg cggagcggcg
    31861 tcttccatgg cgtcaggtca gtctgatgcc gacctcgact ttgcttccgt acagcgcgac
    31921 aacccggaga tggagcgtcg ttgccaggaa gtgatcgacc gttgctggca gcttggtgat
    31981 gctaacccaa ttctgtttat ccacgacgtc ggtgcgggcg gtctttctaa cgcgatgccg
    32041 gagctggtga gcgacggcgg acgcggcggt aaatttgaac tgcgcgatat tctaagcgac
    32101 gaaccgggca tgagcccgct ggaaatctgg tgtaacgaat cccaggaacg ctacgtgctg
    32161 gcggttgctg ccgatcaatt accgctgttt gacgaactgt gtaagcgtga gcgcgcaccc
    32221 tacgcggtga ttggtgaagc gaccgaagaa ctgcatcttt ctctgcacga tcgtcatttt
    32281 gataatcagc cgatcgatct gccgctggac gtcctgcttg gtaaaacgcc gaagatgacc
    32341 cgcgatgtac aaacgctgaa agcgaagggc gatgcgctgg tccgtgaagg gatcaccatt
    32401 gcagacgcgg tgaaacgcgt actgcatctg ccgaccgtgg cggagaaaac tttcctggtg
    32461 accatcggtg accgcagcgt aactggcatg gtagcgcgcg atcagatggt gggtccgtgg
    32521 caggtgccgg tcgctaactg cgcggtcact accgccagcc tcgacagcta ctacggtgaa
    32581 gcgatggcga ttggcgagcg tgcgccggtt gcgctgctgg atttcgccgc ctctgcccgt
    32641 ctggcggtcg gtgaagcgtt aaccaatatt gccgcaacac agattggcga tatcaaacgc
    32701 attaaacttt ccgccaactg gatggcggcg gcaggccacc ctggtgaaga tgcgggcctg
    32761 tatgaagccg ttaaagccgt gggcgaagag ctttgtccgg cgctgggcct gacgatcccg
    32821 gtgggtaaag actctatgtc gatgaaaacc cgctggcagg aaggtaacga agagcgcgaa
    32881 atgacgtcgc cgctgtcgct ggtgatttct gcatttgccc gcgtggaaga tgtacgtcac
    32941 accatcacgc cgcagctttc taccgaagat aacgcactgc tgctgattga tttgggcaaa
    33001 ggcaataacg cgctgggcgc aacggcgctg gcgcaggttt atcgtcagct tggcgacaaa
    33061 ccggcagatg tacgcgatgt cgcgcaactg aaaggcttct atgacgcgat tcaggcgctg
    33121 gttgcacagc gtaagctgct ggcgtatcac gaccgctctg atggcggtct gttggtcaca
    33181 ctggcggaaa tggcctttgc tggtcattgt ggcattgatg cggatatcgg ctctctgggt
    33241 gacgatcgcc tggcggcgtt gtttaacgaa gaactgggtg cggtgattca ggttcgtgcc
    33301 gctgaccgtg aagcggtcga gtccgtactg gcacagcatg ggcttgctga ttgcgtccat
    33361 tatgttgggc aggcggtttc cggtgaccgt tttgtgatta ccgccaacgg gcagactgta
    33421 ttcagcgaaa gccgcactac gttgcgtgtc tggtgggcag aaactacctg gcagatgcag
    33481 cgcctgcgtg acaacccgga gtgtgctgac caggagcacc aggcgaaatc aaacgacgcc
    33541 gatccgggcc tgaatgtaaa actgtcgttc gatatcaacg aagatgtggc agcaccgttt
    33601 attgccactg gcgcgcgccc gaaagttgcc gtactgcgtg agcagggcgt gaactcgcat
    33661 gttgaaatgg cggcagcttt ccaccgtgca ggctttgatg ctatcgacgt gcatatgagt
    33721 gacctgctgg ctggacgcac gggcctggaa gatttccacg ccctggtcgc gtgcggtggt
    33781 ttctcctacg gtgatgtgct gggtgccggt gaaggttggg cgaagtcaat cctgttcaat
    33841 gaccgtgtac gcgatgagtt tgcaaccttc ttccaccgtc cgcaaacgct ggcgctgggg
    33901 gtatgtaacg gttgccagat gatgtctaat ctgcgtgaac tgatcccagg tagtgagttg
    33961 tggccacgtt ttgtgcgcaa tacctccgat cgctttgaag cgcgtttcag cctggttgaa
    34021 gtgacccaaa gcccgtcgct gctgttgcag gggatggtgg gctcgcaaat gccgattgct
    34081 gtctctcacg gtgaagggcg cgtggaagtg cgtgatgcgg cgcatctggc ggcactggaa
    34141 agcaaagggc tggtggcact gcgctatgtc gataacttcg gcaaagtcac tgaaacctac
    34201 ccggctaacc cgaacggttc tccgaacggt attacgtcgg ttacgactga aagtggtcga
    34261 gtcaccatta tgatgccgca cccggaacgt gttttccgta ctgtcagcaa ctcctggcat
    34321 ccggaaaact ggggcgagga tggcccatgg atgcgcattt tccgcaatgc gcgtaagcag
    34381 ttggggtaag tcatcagttc attggttttg cagcccggat gcggcattta cgccgcatcc
    34441 ggcattaagc accaaccact atcataagct cccaactccg ggagcttttt tgtgtctgta
    34501 aatcacgaca atgggtggtt taccgtgtcg ctttctggcg acacttaaat gattgatttt
    34561 attgtaatct aataagttta tcttaagttg tctcttttta gcgacacagt ggctgattta
    34621 cattcaaata gcgaagaagc cataaatccc tcaaagcaac gtataatcag tacgttacca
    34681 aactattttc tttattggca cagttactgc ataatagtaa gcagtggctc attcaccgac
    34741 ttatgtcagc cccttcggga tgtgctacat aaaattcgaa tgacgcacaa caaggtgcct
    34801 gccgtccaac ttctgatatc agcgtagcta tatcaaccat cgggcgaaac gtcgagttag
    34861 gcaccgcctt attccataac aaagccgggt aattcccggc tttgttgtat ctgaacttcc
    34921 cctcggttag catcaggcta ttcgcgtctg acgagagtaa caccttgaaa cgctggcccg
    34981 tttttccccg ctcattacga caactggtaa tgctggcatt tttgctaatt ctactacccc
    35041 tgttggtgct ggcatggcaa gcctggcaaa gcctgaatgc gcttagcgat caggcggcgc
    35101 tggttaaccg cactacgctt attgatgccc ggcgtagtga agcgatgacc aacgcggcgc
    35161 tggagatgga gcgtagctac cgtcagtatt gcgtgctgga cgaccctacg cttgcgaagg
    35221 tttatcaaag ccagcgcaag cgttacagcg aaatgctcga tgcccacgca ggcgtactgc
    35281 cagacgataa actttaccag gcattgcgtc aggatttgaa caatctggcc caacttcagt
    35341 gtaacaacag tggtcccgat gctgccgccg ccgcgcgtct tgaagccttt gccagtgcca
    35401 ataccgaaat ggtgcaggcc acgcgcacag tggtgttctc tcgtgggcag caacttcagc
    35461 gtgaaatcgc cgaacgtggg caatattttg gttggcaatc gctggtgcta tttctggtga
    35521 gtctggtgat ggtgctactt ttcacgcgga tgattatcgg gccggtgaaa aatatcgagc
    35581 gcatgatcaa ccgtctgggg gaagggcgtt ctctgggcaa tagcgtttcg ttcagtggac
    35641 cgagcgagtt acgctcggtt gggcaacgta ttctttggtt aagtgagcgc ctgtcatggc
    35701 tggaatccca acgccatcaa tttttaagac atttatctca tgaattaaaa acgccactgg
    35761 cgagtatgcg cgagggcact gaattactgg ctgaccaggt tgtcgggccg cttacgccag
    35821 agcaaaaaga ggtggtgagc attcttgata gcagcagccg caatttgcaa aaactgatcg
    35881 aacaactgct tgattacaac cgtaaacagg cggacagtgc ggtggaactg gagaatgttg
    35941 aattagcacc gctggtggag acagtggttt ctgctcatag cctgcccgca cgggctaaaa
    36001 tgatgcatac cgacgtcgat ctcaaagcaa cagcttgcct ggcggagcca atgctgctga
    36061 tgagcgtact ggataatctt tactccaatg cggtgcacta cggggctgaa tccggtaaca
    36121 tttgccttcg cagcagctta catggtgcac gggtttatat tgatgtcatc aatacaggca
    36181 cacccattcc gcaagaggaa cgcgccatga tcttcgaacc cttttttcaa ggaagccacc
    36241 agcgaaaagg ggcggtgaag ggcagcggtc tgggattaag cattgccagg gattgtattt
    36301 gccgtatgca aggggaactg tatctggtcg acgagagcgg gcaagacgtt tgtttccgca
    36361 ttgaattgcc gtcgtcgaaa aacacgaaat aatgctaaca gcctatccca tcaggctatt
    36421 ttatttgcca gttcggcccc gggcagtgct cgaaatcctc acgtacgacg tgtacgctcc
    36481 ggtttctccg cgctgtccat gtccaaactg cctgcaataa ttacgcccac tgggataggc
    36541 attaagtctg gtgaatatgc gacacatttt tcaacgatta ttgccccgac ggctgtggct
    36601 ggcagggctg ccatgtctgg cattgctggg ttgcgtgcag agtcacaata agccagccat
    36661 tgatacgcca gcagaagaaa aaattccggt ttatcaactg gctgactatc tttctactga
    36721 atgtagcgac atctgggcgc tgcaaggtaa atcaaccgaa accaatccgc tttactggct
    36781 gcgggcgatg gattgtgctg atcgtttaat gcctgcgcag tcacgtcagc aagctcgcca
    36841 atatgacgac ggcagctggc aaaatacctt taaacaaggg attttgctcg ccgacgccaa
    36901 aattacgccg tatgagcgcc gccagctggt cgcgcgcatt gatgcgctaa gtaccgagat
    36961 cccggcacag gttcgccctc tgtatcaact ctggcgcgac ggtcaggcgc tgcaactgca
    37021 actggcggaa gagcgccaac gctacggcaa gctccagcag gccagcgaca gcgagctgga
    37081 tacattgcgc cagcaacatc acgttctaca gcagcaactg gaactcacca cccgcaagct
    37141 ggaaaacctg actgatattg aacgccagct ctcgacccgc aaaccggctg gaaatttctc
    37201 gccggatacg ccgcacgagg gtgaaaagcc cgcgccatcc acccatgagg tcactcctga
    37261 tgagccataa acctgcgcat ttattattgg tcgatgacga tccgggattg ctgaaactgc
    37321 ttggcctgcg cctgaccagc gaaggctaca gcgtggtcac ggcggaaagt ggcgctgaag
    37381 gattacgggt actgaatcgc gaaaaagtag atttagtcat cagcgacctg cggatggatg
    37441 aaatggacgg tatgcagctg tttgctgaaa ttcagaaagt gcagccggga atgccagtaa
    37501 ttattcttac cgcgcatggt tctattcccg atgccgttgc tgcaacacag cagggcgtgt
    37561 tcagtttcct caccaagcct gtcgataaag atgcgctata tcaggcaatt gacgatgcgc
    37621 tggaacaatc cgcgccagcc accgatgaac gctggcgcga ggcgattgtc acccgcagcc
    37681 cgctgatgct gcgtttgctg gaacaggcgc ggctggtggc gcaatcagac gttagcgttt
    37741 tgattaacgg ccagagcggc accgggaaag agattttcgc ccaggctatc cacaacgcca
    37801 gcccgcgcaa tagcaaacca tttattgcta ttaactgtgg cgcattgccc gagcaattgc
    37861 tggagtcgga gctgtttggt catgcgcgtg gcgcgtttac tggcgctgtc agcaatcgcg
    37921 aaggtttatt ccaggcggcg gaaggcggta cgctatttct cgatgagatt ggcgatatgc
    37981 ccgcaccgtt gcaggtcaaa ctgctgcgcg tgttgcagga gcgtaaagtg cgcccgctgg
    38041 gcagtaaccg cgatattgat atcgacgtgc ggattatttc cgccactcac cgcgacctgc
    38101 caaaagcgat ggcgcgcggg gaattccgtg aagacctcta ttaccgcctc aacgttgtca
    38161 gcctgaaaat tccggctctg gcagagcgca cagaagacat tccgcttctg gcaaatcacc
    38221 tgttgcgcca ggcggcagag cgacataaac cgtttgtccg cgcgttctcc actgatgcga
    38281 tgaaacgcct gatgaccgct agctggccgg gtaatgtgcg ccagttggtc aacgtgattg
    38341 aacagtgcgt ggcgctgacc tcatctccgg ttattagcga tgcattagta gagcaggcgc
    38401 tggagggtga aaatacggcg ctgccaacct ttgttgaggc acgtaatcag tttgaactca
    38461 actatttgcg taagctgctg caaatcacca aaggcaacgt cacacacgcg gcgagaatgg
    38521 cggggcgcaa ccggacagaa ttttataaac tgctttcccg acacgagctg gatgcaaacg
    38581 atttcaagga atgaattggc gttatgtgtt acgtttagca gatcaaaaga caggcgacct
    38641 tttcaaggaa tagcatgaaa aagattgatg cgattattaa acccttcaag ctggacgatg
    38701 ttcgcgaagc actggctgaa gtcggcatta ccggcatgac ggtcactgag gtgaaaggtt
    38761 ttggccgcca gaaaggccat accgagctgt accgcggcgc ggagtatatg gtggattttc
    38821 tgccgaaagt gaaaattgag attgtcgtac cggacgacat tgtcgatacc tgtgttgata
    38881 ccattattcg cacggcgcaa accggcaaaa tcggtgacgg taaaatcttc gtcttcgacg
    38941 tggcacgggt cattcgcatc cgtaccggtg aagaggacga cgcggcaatt taaaccgcgt
    39001 caagggttgc cggatgtttc catccggcaa catcaaatta cagcacctta tgcgggccaa
    39061 agcattcgta atgaatgttt tcctgcttca cgcccagatc cactaactgt ttcgcggtaa
    39121 actgcatgaa gccaaccggg ccgcagagat agaactgcat tgtcggatcg ctgaacgcac
    39181 cttccagttt gctcaaatcc atcagacctt cgctatcaaa ctgaccttta gcgcgatcgg
    39241 cttcgctcgg ctgacgatac caggtgtgcg cggtaaagcg cggcagtgac tgccccagtt
    39301 ccttaacttc atcagcaaag gcgtgaacat cgccattttc tgccgcatgg aaccagttca
    39361 cttgtgctgt gtggcctgct tttgccagcg tgtcgagcat tgccagcatt ggcgtttgac
    39421 caacaccggc agagattaac gtcactggtg tgtcatctgc gacagccata aagaaatcac
    39481 ctgccggagc gaccagtttc acgacatcgc caacattggc gtgattgtgc aaccagttgg
    39541 atacctgccc accctcttcg cgtttcaccg caatacgata gcctttgcca tccggtttgc
    39601 gagtcaaaga gtactgacga atttcctgat gcgggaaacc ttccggcttc agccagacgc
    39661 cgagatattg ccccggacgg tattctgcca ctgcgccacc gtcgaccggc tccagttcga
    39721 agctggtgat aagcgcgctg cgcggtgttt tagccacaat gcggaaatcg cgagtacctt
    39781 cccaaccacc ggctttgctg gcgttttcgt tatagatttc cgcctcgcga ttgataaata
    39841 cattagccag tacaccatag gctttacccc acgcgtccag cacttcctgc cccgggctga
    39901 acatttcgtc cagcgttgcc aacaggtgtt caccgacgat gttgtactgt tccggtttga
    39961 tctggaagct ggtgtgcttc tgcgcgattt tttctaccgc tggcagcagc gcaggcaggt
    40021 tttcaatatt actggcgtag gcggcaatag cgttaaacag ggcttcacgt tgatcgccat
    40081 tacgctggtt actcatgtta aaaatttctt tgagttctgg gttatgagta aacatacggt
    40141 cgtagaaatg ggcggttaac tttggccccg tttccaccag taaagggatg gtggctttta
    40201 ctgtagcgat ggtttgagcg tcaagcatat ggtcttcctt tttttgcatc ttaattgatg
    40261 tatctcaaat gcatcttata aaaaatagcc ctgcaatgta aatggttctt tggtgttttt
    40321 cagaaagaat gtgatgaagt gaaaaatttg catcacaaac ctgaaaagaa atccgtttcc
    40381 ggttgcaagc tctttattct ccaaagcctt gcgtagcctg aaggtaatcg tttgcgtaaa
    40441 ttcctttgtc aagacctgtt atcgcacaat gattcggtta tactgttcgc cgttgtccaa
    40501 caggaccgcc tataaaggcc aaaaatttta ttgttagctg agtcaggaga tgcggatgtt
    40561 aaagcgtgaa atgaacattg ccgattatga tgccgaactg tggcaggcta tggagcagga
    40621 aaaagtacgt caggaagagc acatcgaact gatcgcctcc gaaaactaca ccagcccgcg
    40681 cgtaatgcag gcgcagggtt ctcagctgac caacaaatat gctgaaggtt atccgggcaa
    40741 acgctactac ggcggttgcg agtatgttga tatcgttgaa caactggcga tcgatcgtgc
    40801 gaaagaactg ttcggcgctg actacgctaa cgtccagccg cactccggct cccaggctaa
    40861 ctttgcggtc tacaccgcgc tgctggaacc aggtgatacc gttctgggta tgaacctggc
    40921 gcatggcggt cacctgactc acggttctcc ggttaacttc tccggtaaac tgtacaacat
    40981 cgttccttac ggtatcgatg ctaccggtca tatcgactac gccgatctgg aaaaacaagc
    41041 caaagaacac aagccgaaaa tgattatcgg tggtttctct gcatattccg gcgtggtgga
    41101 ctgggcgaaa atgcgtgaaa tcgctgacag catcggtgct tacctgttcg ttgatatggc
    41161 gcacgttgcg ggcctggttg ctgctggcgt ctacccgaac ccggttcctc atgctcacgt
    41221 tgttactacc accactcaca aaaccctggc gggtccgcgc ggcggcctga tcctggcgaa
    41281 aggtggtagc gaagagctgt acaaaaaact gaactctgcc gttttccctg gtggtcaggg
    41341 cggtccgttg atgcacgtaa tcgccggtaa agcggttgct ctgaaagaag cgatggagcc
    41401 tgagttcaaa acttaccagc agcaggtcgc gaaaaacgct aaagcgatgg tagaagtgtt
    41461 cctcgagcgc ggctacaaag tggtttccgg cggcactgat aaccacctgt tcctggttga
    41521 tctggttgat aaaaacctga ccggtaaaga agcagacgcc gctctgggcc gtgctaacat
    41581 caccgtcaac aaaaacagcg taccgaacga tccgaagagc ccgtttgtga cctccggtat
    41641 tcgcgtgggt actccggcaa ttacgcgtcg cggcttcaaa gaagcagaag cgaaagaact
    41701 ggctggctgg atgtgtgacg tgctggacag catcaatgat gaagctgtta tcgagcgcat
    41761 caaaggtaaa gttctcgaca tctgcgcacg ttatccggtt tacgcataag cgaaacggtt
    41821 aatctgttga aaaaaggctt ccttcgggaa gcctttctac gttattgcgc catcaaatct
    41881 gccgtaactg cgcctcaaca tacaaatagc caattcccag cacctgttgt gcgcggctta
    41941 attgcccgaa gccaatttgc gtcgctttca cgctcggtcc ttcgtcgcgg tcgaacgggt
    42001 taaatcctgt ctggcgaata atagtattaa gccagttctc accaaaggca caactgcgac
    42061 cgtacaacca aatctgatta atattgagga tgttgaggaa gttatacaga ctcaaaccaa
    42121 tggcattggc actgcggtca acccagctgg tgatccacgg ttctccactt tgccaggcag
    42181 cgattaactg cgctgtagtc agtttttcag gatcaagttg agtactaacc ggttgtgatt
    42241 ttagccatac ccgcgcctgt ttttttaatg cgcttaacga ggcgacggtt tccaggcagc
    42301 cataacgtcc acagtcgcag acgacgccat ccggattaac gatggtgtga ccaatctgtc
    42361 cgctaccata caaactgccg cgataaattt gctcgttaat cacgaacgac gagccaatgc
    42421 cgtaatcaac gttgatcacg cagaaatccc gttcctgcga attattttgc catttctccg
    42481 ccagcgccag catcacgcag tcattatcga ccatcacccg aatgccgagc ttctcttcca
    42541 gcagatattt cacctccacc ggcgttgtcc acggcgcttg cggcatggtt tgcgacacgc
    42601 cggtcacagg atcaacctga ccgtggattg ccagcgccag attgatggtg cggtccggcc
    42661 acaatttacg gtggcgatgc cagcattttt cgatttcgga cagcagcgcc tgcggcgtcg
    42721 gtgcatcaat ctgtaaatac tcaaattcac ctttcggact taaacaagca ttagcaacct
    42781 gacactcaat actggtgggc gtcacgttca ggcacagcgt ccagtcacct tccggcgcaa
    42841 tcagccatgt accgctacta tgcccgcgcg tctggctttc atcgtcaata ttcaccaccc
    42901 gtttttcgct ttccagttcc tgcaaaatat tactgactgc cggaatcgaa atttgcgcca
    42961 gccgggccag cgttgattta ttggcgcgct tttgccggta cagcagttcc agaatcacgc
    43021 atttgttatg gtggcgaatc tgttgattgt taatgcaggc tctcatcttc actaaacctt
    43081 gtttagttaa ttgcgtgatt attccgcgaa aacattgcca gattgttcaa tacactgcca
    43141 caaatctttt aattcagtat gtcttgttaa tattgagggc accatgactc cagtaaaagt
    43201 gtggcaagag cgcgttgaga tcccgaccta tgaaaccggg ccgcaggata tacatcccat
    43261 gttcctcgaa aatcgcgttt atcagggatc gtccggcgcg gtttatccct acggcgtgac
    43321 cgatacgctg agcgagcaga aaaccctgaa atcctggcag gcggtgtggc tggaaaacga
    43381 ctacatcaaa gtgatgatcc tgccggaact gggcggtcgg gtgcatcgcg cgtgggataa
    43441 agtgaaacag cgcgattttg tttatcacaa tgaagtcatt aaacctgcgc tggtggggct
    43501 gctggggccg tggatttccg gtgggattga gtttaactgg ccgcaacatc atcgcccgac
    43561 cacctttatg cccgttgatt tcaccctcga agcccatgaa gacggtgcac agacggtgtg
    43621 ggtaggcgaa acggagccga tgcatggttt acaggtgatg acaggtttca ccctgcgccc
    43681 tgaccgggcg gcgctggaaa tcgccagccg cgtctataac ggtaacgcca cgccgcgtca
    43741 tttcttgtgg tgggccaacc cggcagtgaa agggggggaa gggcatcaga gcgttttccc
    43801 accggatgtg actgcggtgt ttgatcacgg caaacgggcc gtctccgctt tccccatcgc
    43861 caccggcact tactacaaag tggactactc cgccggagtg gacatttctc gctataaaaa
    43921 tgtgcctgtt ccaacctcat atatggccga aaaatcgcag tacgattttg tcggcgcgtg
    43981 gtgtcacgat gaagatggcg gtttgctgca cgttgccaac caccatattg cgccaggtaa
    44041 aaaacagtgg agttggggac acagtgaatt tggccaggcg tgggataaga gtctgaccga
    44101 caataacggt ccgtatatcg aactgatgac cggtattttt gccgataacc agcccgattt
    44161 tacctggctt gatgcttacg aagagaagcg ttttgagcag tatttcctgc cttatcattc
    44221 tttgggcatg gtgcaaaatg cctcccgcga tgcggtgata aaactccagc gtagtaagcg
    44281 ggggattgag tgggggctgt atgccatctc tccgttgaac ggataccgcc tggcgatccg
    44341 cgaaatcggc aaatgcaacg cgttacttga tgatgccgtg gcactgatgc ctgcgaccgc
    44401 catccagggc gtgttgcacg gtatcaatcc tgaaaggctg accattgagc tctccgatgc
    44461 cgacggcaat attgtactga gttatcagga acatcagccg caagagttgc cgttgccgga
    44521 cgtcgccaaa gcgccactgg cagcacaaga cattaccagt acagatgaag cctggtttat
    44581 cggtcagcat ctggagcaat atcatcacgc cagccgttca ccgttcgatt actacctgcg
    44641 cggcgtggcg ctggatccgc tggattaccg ctgtaacctg gcgctggcga tgctggaata
    44701 taaccgcgcc gatttcccgc aagcggtggc gtatgccact caggctctga aacgcgcaca
    44761 tgcgctgaac aaaaatccgc agtgcggaca ggcgagtttg attcgcgcca gtgcttacga
    44821 acgtcaggga caatatcaac aagccgaaga ggatttctgg cgtgcggtct ggagcggcaa
    44881 cagcaaagcc ggtggctatt atggcctggc acgactggcg gcgcgtaatg gtaacttcga
    44941 cgctggtctg gatttttgcc aacaaagtct tcgcgcctgc ccaaccaatc aggaagtgct
    45001 ttgcctgcat aatctgctgc tggtgttaag tggtcgtcag gacaacgcgc gtgtgcagcg
    45061 cgagaaactg ctgcgcgatt atccgctgaa cgccactctg tggtggctga actggttcga
    45121 tggtcgtagc gaatcagctc tcgcgcagtg gcgcggtctg tgtcagggac gcgacgttaa
    45181 cgccctgatg accgccgggc aactgattaa ctggggaatg cccaccctgg cggcagagat
    45241 gctgaacgca ctggactgcc agcgcacgct gccgctttac ctgcaagcca gcttgctgcc
    45301 gaaagccgaa cgtggcgaac tggtcgcaaa agccattgat gtcttcccgc agtttgtccg
    45361 tttcccgaat acgctggaag aagtggcggc gctggagagt attgaagagt gctggtttgc
    45421 tcgccattta ctggcctgct tctactacaa caaacgtagc tacaacaaag ccattgcctt
    45481 atggcaacgt tgcgtagaga tgtcgccgga gtttgccgac ggctggcgcg ggttagcgat
    45541 ccatgcgtgg aataagcaac acgattatga gctggccgcg cgttatcttg ataatgctta
    45601 tcagcttgcg ccgcaggatg cacgtctgct tttcgaacgg gatttgcttg ataagttaag
    45661 tggagccaca ccggagaaac gactggcgcg tctggaaaat aatcaggaaa ttgcgctgaa
    45721 acgcgacgac atgaccgcag aactgctcaa tttgtggcat ctcacgggtc aggcagacaa
    45781 agcggcggac attctcgcca cgcgtaaatt ccacccgtgg gaaggcgggg aagggaaggt
    45841 caccagtcag tttatcctca accagttatt acgcgcctgg cagcatcttg atgccagaga
    45901 gccgcagcag gccagcgaac tgcttcatgc cgcgctgcat tatccggaga atttaagcga
    45961 aggccgttta ccggggcaaa ctgataacga catctggttc tggcaggcga tatgcgccaa
    46021 cgcgcagggc gatgaaactg aagcgatgcg ttgtttacgt ctggcggcga ccggcgatcg
    46081 caccattaac atccacagtt attacaacga tcagccggtt gattatctct tctggcaagg
    46141 aatggcgctg cgactgctgg gtgaacagca aaccgcacag caactgttta gtgaaatgaa
    46201 acagtgggcg caagagatgg cgaaaaccag tattgaggcg gatttctttg ctgtttcaca
    46261 acctgacctg ttgtcgctgt atggcgattt acaacagcag cataaagaaa aatgcctgat
    46321 ggtggcgatg ctggcgtccg cgggactcgg ggaggttgcg caatatgaat ctgctcgcgc
    46381 tgaattgacg gcgattaatc cggcctggcc gaaagcggca ttattcacca ccgtgatgcc
    46441 ttttattttt aactacgttc actaaaccct attaactgta atatttgagc ggcacgcgct
    46501 gccgctcatc acaaaataat tagaataagc cgttgttaat aaaaacccta caggaggcac
    46561 tatgcctaaa aaaatgagaa caacccgtaa tctattatta atggctacgc tattaggtag
    46621 cgcactattt gccagggctg cggataaaga aatgaccatt ggtgcaatat accttgatac
    46681 ccagggatat tacgctggag tgcgtcaggg cgttcaggat gcggcaaaag attcttcagt
    46741 acaggtacag ttaattgaaa ctaacgccca gggtgatatt tcgaaagaaa gtacctttgt
    46801 tgataccctc gtggcgcgta atgtcgatgc cattatttta tcggcagtgt ctgaaaatgg
    46861 cagtagccgt accgttcgtc gcgccagtga agcgggtatt ccggtgattt gctacaacac
    46921 ctgtattaat caaaaggggg gcgataaata tgtctcggcg tatctggtcg gcgatccgct
    46981 ggaatttggt aaaaaactgg gtaacgctgc cgccgattat tttattgcca ataaaattga
    47041 ccagccgaaa attgctgtca tcaattgcga agcctttgaa gtttgtgtgc agcgacgtaa
    47101 aggatttgaa gaagtattaa aatcccgcgt tcccggcgcg caaattgtcg ctaatcagga
    47161 agggactgtt ttagataaag cgatttccgt tggtgaaaaa ctgattatct ccacgccgga
    47221 tctcaacgcc attatggggg agtcgggcgg tgcgacactc ggcgcggtaa aagcggtacg
    47281 taatcaaaat caggccggaa aaattgctgt tttcggttcg gatatgacaa ccgaaattgc
    47341 tcaggagctg gaaaacaatc aggtgctgaa agcggtagtg gatatttccg gtaagaaaat
    47401 gggcaatgct gttttcgcgc aaacattgaa ggttatcaat aaacaagccg acggtgaaaa
    47461 agtgattcag gtgccaatcg atctctatac caaaacggaa gatggtaaac agtggctggc
    47521 aacgcacgtt gatggtctgc cctaatcgct gattcgcaga ggtccctatg ttcacggcaa
    47581 cagaggcagt cccggtagca aaagtggtgg caggaaataa gcgttacccc ggcgtcgttg
    47641 cgctggataa cgttaacttc acgctcaata aaggcgaagt tcgtgcgctg ttaggtaaaa
    47701 acggcgcggg caaatcgact ctcattcgaa tgcttaccgg cagcgaacgc ccggatagcg
    47761 gtgatatctg gattggcgag acgcgactgg aaggtgacga agctacgttg actcgccgtg
    47821 ccgctgaact gggggttcgt gcggtttatc aggagttaag tctggtggag ggactgacgg
    47881 tggcggaaaa cctctgcctc ggtcagtggc cccgccgcaa cggcatgatt gattacctgc
    47941 aaatggcgca ggatgcccaa cgttgcttac aggcgctggg cgttgacgtt agccctgaac
    48001 aacttgtttc aacgctaagc ccggcgcaaa agcaactggt ggaaattgcg cgggtgatga
    48061 agggcgagcc gcgcgtggtc attcttgatg aacctaccag ttcgcttgcg agtgcggaag
    48121 ttgaactggt gatcatcgcg gtgaaaaaga tgtcggcact gggcgtggcg gtgatttatg
    48181 tcagccaccg gatggaagaa attcgccgca ttgcctcctg tgccaccgtt atgcgcgatg
    48241 gtcaggtggc gggcgatgtg atgctcgaaa acacctccac gcatcatatt gtgtcgctaa
    48301 tgctagggcg tgatcacgtt gatattgcgc cggttgcccc tcaggaaatt gtggatcagg
    48361 ccgtgctgga agtccgtgca ttacgccata agcccaagct ggaggatatc agctttacgt
    48421 tacgtcgcgg cgaagtgctt ggcattgctg gcctgctggg ggcagggcgc agtgaattgc
    48481 tgaaggcgat tgttgggctg gaggagtatg aacagggcga aattgttatc aacggcgaga
    48541 aaatcacgcg ccccgattac ggcgacatgc tgaaacgcgg cattggctat acgccagaaa
    48601 accgcaaaga agcggggatc attccctggt tgggcgttga cgaaaataca gtgctgacca
    48661 atcggcaaaa aatcagcgcc aacggcgtac tgcaatggtc caccatccgc cgcctgaccg
    48721 aagaggtgat gcagcggatg acggtcaagg ccgccagtag cgaaacgccc atcggcacgc
    48781 tttctggcgg caatcagcaa aaagtggtga tcggtcgttg ggtctatgcc gccagccaga
    48841 ttttgttgct cgacgagcca acgcgcggcg tcgatatcga agccaaacag cagatttacc
    48901 gtattgtccg tgagctggct gccgaaggaa aaagcgtggt gtttatctcc agtgaagtgg
    48961 aggagttgcc gctggtctgt gaccgcatcc tgttactaca gcacggcacg ttctcgcagg
    49021 agtttcacgc tccggtcaat gtggatgagc tgatgtccgc cattctgtct gtgcactgat
    49081 gaattttacc gaggaacctg aagatgtctg cttcgtcatt accattgccg cagggcaaga
    49141 gcgtctcgct caaacaattt gtcagtcgcc atattaatga gatcggtttg ctggtggtga
    49201 ttgccattct ttatctggtc ttctccctga acgcgccagg ctttatctca ttgaataacc
    49261 agatgaacgt gctgcgcgat gccgccacca ttgggattgc cgcctgggcg atgacgctga
    49321 ttattatctc cggtgaaatt gatgtcagcg ttgggccgat ggtggctttt gtctcggtgt
    49381 gcctggcatt tttgctgcaa tttgaagttc cgctggcggt ggcgtgtctg ctggtgttgc
    49441 tgttaggcgc gctgatggga acgctcgccg gggtgctgcg cggcgtgttt aacgtaccaa
    49501 gtttcgttgc cacactgggg ctgtggagcg ccctgcgcgg aatggggctg tttatgacca
    49561 acgcactgcc agtgccgatt gacgaaaacg aggtgctgga ctggctgggc ggacaatttc
    49621 tcggtgtgcc agtatccgcg ctgatcatga tggtgttgtt tgcgctgttt gtgttcatta
    49681 gccgaaaaac cgccttcggg cgctcggttt ttgccgttgg cggtaatgcc acggcggcgc
    49741 agttgtgcgg tattaacgtt cgtcgagtgc gcattcttat ctttaccctt tcgggattat
    49801 tagcggcggt gaccggcatt ttgctggcgg cacgcctcgg ttccggtaac gcaggtgccg
    49861 caaacggtct ggagtttgac gtcatcgccg cggtcgtggt cggcggtacg gcactttccg
    49921 gcggtcgcgg ttccctgttc ggtacattgc ttggtgtgct ggtgattacg ctaatcggta
    49981 acggtctggt gctgctcggt attaactcct ttttccagca ggtggtacgc ggcgtcatca
    50041 tcgtggtggc ggtgctggcg aatatcttgc tgacccagcg aagcagtaaa gcgaaacgct
    50101 aaaccttaac gcccctggcg cgggtgcggc agggatgaaa attaatcgta ttgttaatga
    50161 gggatttatg aaaacgatgc tggcagctta tttaccagga aattcaaccg tcgatctgcg
    50221 ggaagttgcg gtgccgacgc cggggattaa ccaggtactg atcaaaatga aatcctccgg
    50281 gatttgcgga agcgatgtcc actatatcta tcatcaacac cgtgccacgg cggcggcacc
    50341 cgataaaccg ttataccagg gatttatcaa cggtcatgaa ccgtgcgggc agattgtggc
    50401 gatggggcaa ggctgccgcc attttaaaga gggcgaccgc gtgctggtgt atcacatttc
    50461 tggctgtggt ttttgcccga actgccgccg cggctttcct atctcttgta ctggcaaagg
    50521 aaaagcggct tacggctggc agcgtgacgg cggtcatgcc gaatatctgc tggcggaaga
    50581 aaaagatctg atcctcctgc cggatgcgct gagctacgaa gatggtgcgt ttatcagttg
    50641 cggcgttggt acagcgtatg aaggaatttt gcgcggcgaa gtttccggca gtgataacgt
    50701 gctggtggtc ggtctggggc cagtcggcat gatggcgatg atgctggcga aaggtcgcgg
    50761 tgcaaaacgg atcatcggcg ttgatatgct gccggaacgt ctggcgatgg caaaacagtt
    50821 aggggtgatg gatcacggct atttagcaac caccgaaggt ctgccgcaga ttatcgccga
    50881 actcacccac ggtggcgcgg atgttgcgct cgattgttct ggtaatgccg caggtcgctt
    50941 gctggcactg cgatccaccg ctgactgggg acgggtggtt tacatcggag aaaccggaaa
    51001 agtggagttc gaggtcagcg ccgatctgat gcaccatcaa cggcggatta tcggctcctg
    51061 ggtgaccagt ctgttccata tggaaaaatg cgcccatgat ctgacggact ggaaactgtg
    51121 gccgcgtaac gccattaccc atcgcttctc gctggaacag gcaggtgatg cctatgcgct
    51181 gatggcgagc ggcaaatgcg ggaaagttgt gattaacttc ccggattaag gagttagcgg
    51241 atgaccatct ataccttatc ccacgggtcg ctaaagctgg acgtttctga ccagggcggc
    51301 gtaatcgaag gcttctggcg cgatacgacg ccgctcttac gtcctggtaa aaaaagcggt
    51361 gtggcaaccg atgcctcatg ttttccgctg gtgccatttg caaaccgggt gagcggcaat
    51421 cgttttgtct ggcaggggcg tgagtatcaa ctgcaaccga atgttgagtg ggatgcgcac
    51481 tatctacacg gcgatggctg gctgggcgag tggcaatgtg tctcgcacag cgatgatagt
    51541 ttgtgtctgg tgtatgaaca tcgcagcggt gtctatcact atcgggtaag tcaggcgttt
    51601 catttaacgg cggatacgct gacggtgacg ctctctgtca ccaatcaagg ggcagagacg
    51661 ctgccatttg gtaccggctg gcatccttat tttccgttgt cgccgcaaac gcggattcag
    51721 gcacaggcga gcggttactg gctggagcgg gagcagtggc tggcgggtga gttttgcgag
    51781 cagctaccgc aggaactgga ttttaaccag cccgcgccgt tgccgcgcca gtgggtgaac
    51841 aatggttttg ccggatggaa tggtcaggcc cgcatcgagc agccgcagga aggttatgcc
    51901 atcatcatgg aaacgacgcc acctgcaccg tgttatttca tctttgtttc cgaccctgcg
    51961 tttgataaag gatatgcgtt tgatttcttc tgtctggaac cgatgagcca tgcgccggat
    52021 gatcatcatc gcccggaagg gggtgatctc attgcgctag caccagggga atcaacaact
    52081 tcagagatgt cgttgcgggt agagtggctg taatttatta aatgccggat aaggcgttca
    52141 cgccgcatcc ggcatctttc ctaacgccga tcgagagaaa ttgcccccgg cccggtaatt
    52201 gccagcagca ggaatgcgcc agcgatactg acattcttcc agaaattaat catatttggc
    52261 ccaaccgcat cgccggtcat atcccagtaa tggtgaccaa tcaccgccgt acccagcgtg
    52321 tagaaaataa acagcaccgc cagcggacgg gtgaaaaagc caagcacgat taatatcgcg
    52381 gcgggcactt ccataactac cgcaataatc gctgccagca tcggcattgg cgcgcccaac
    52441 gaggccatat attggaccgt accgtcaaag cccatcattt tgggaaaacc aaaaataatg
    52501 aaaattaaga ccacggcgat acgggcaatt aataacaaaa cggggcgggc agctccaaaa
    52561 tcaaaataac gtagtgtgtt cataaccaat ccctttgcaa gataatgtga tttaaacgta
    52621 atacacactc ttgcacctcg ccacgcccca ggcattgaga tattcgctaa ttatcctggt
    52681 tacagtgatt taagcgcgat gttctcatct atcagcagtt tcgcatcaaa cgttttgccg
    52741 ctctggatcc atttgcgaat tgggcgaatc tcacgcccct gattcagcgt gaccgtaccg
    52801 ataagcacgc cgttttgcag attaaaccaa atcgccttct gagtttccgg gttgccacga
    52861 caaagccagt catcgccacg catatcgcca ataaactgta agttatcact gtactgatcg
    52921 ctccagaacc acggcggcgg cagtagcggt agcggtagcc ctaacattgc ggcagcggca
    52981 atttgcgcct ggttattggc gttttcccag ctttcgcagc ggtgtagtgc accattatca
    53041 agacgagtga ttgccacatc gccaccggca aagatcgcgg gatcgcaggt gcggcaagcc
    53101 tcatcaatga caatgccatt ggcagtatca aggttggcct cgcgagccag ttgctcgttg
    53161 gcgctgatac caataccgta aatcaccaca tcagcctgaa gcgtttcccc actttgcagc
    53221 gtcagttcta ctttttcacc atcgaccaca tgttcaatgg cattattgag cagaatgcgc
    53281 acaccagcct gctggtggcg ctgtaaaaga tagcgttgca cgggcggtgg tgcattacgg
    53341 cccatgacgg ttgccgccag ttcaatcact gtcaccttac atctgcgctg cgtggcgctg
    53401 gcagccagtt ccagaccaat agttccggca ccgacaatca cgactgaccg ttcgggctgc
    53461 agaacttctc gcagtctggc ggcatcaccg gcatggcgta gggtaaagca gcgttctccc
    53521 agtgcatcaa gcaacggcag cggtcgagct gccgcgccgg ttgctataaa aagctgatcc
    53581 cagtgccagc tttcgccgtt ggttaacact aactctcgtg tgtcgcgacc cagcgttttg
    53641 atggttacac cggaatgcag atgaacattg ttttcctgcc accagttagc gggtaacacc
    53701 tgctgtaact gcggggaatc ttccagcaac atggatttcg agagtggagg tcgttcataa
    53761 ggaagatgtc gctcatcgga aaacagatgc agctcaccgg tgaacccttg ctggcgtagc
    53821 gaggccgcag ccattgccgc cgcttgcccg ccaccgacaa taatgatggt tttttctttc
    53881 atcgtgcttc cttaaagatc cagcccagcc gctacgtggc gaatgccgcg aatcgctaaa
    53941 cccgcatcag cgttgatcat cacaccgctt aatgcgcgat tattgcgccg cgatgccaac
    54001 atcacatacg gccccgtaaa atccgccggt tgcgggaaaa attgcagcgg taaaatggcg
    54061 gcaattttct ccggcgtcag agactgcatt atcgaggttt cactttgccc gagcgcctgt
    54121 gggccgcgca ggtcgctggc cataccacac gggccgacgc cattcacccg cactttcggt
    54181 gccagttcat aagccagttg gcgaataagt ccggttgcgg catgtttact ggcggtgtac
    54241 agcgggccac cgccgccagg ataccaggcg gcatttgaca gtgtgaaaat catgctgcct
    54301 tcactggcga ttaacgccgg agcgcaggct tttgcgccca gcaggtaacc gagaacattg
    54361 acgttaaaca gctcgtggaa gccggtttcg agcgtctctg cgggagtatt aaccagtgag
    54421 gcattgtgat cccagatgcc tgcattgccg ataaaacaat ccagcttgcc ggaacgagtc
    54481 aggatctgat cgaccgcgcg ttgataatcg gcataacagg tcacgttacc ttccaccgcc
    54541 agaatatgtt cgccaaatcg ctgacgcaga ctggcgactt ttgccgccga cagttccagc
    54601 gtggcaacct gcgcgccttc ttcgataaat cgctcgacca gcgccagccc taatcccgat
    54661 ccgccgccgg taataaaaat ggactcgtta tgcagatcgc tcatggctgc gcctccggta
    54721 agtcgatgaa aatgtcacca ccttcaacgt gtactggata agtggtgagc ggatcggtgg
    54781 cgggcaggca taacgctttc cccgttttca ggcaaaaact ggcggcgtgt agcgggcact
    54841 ccaccgtggc gtcatcttcc agataccctt ctgacattga cgcattacca tggctgcaac
    54901 gatcgttaat ggcataaaac tcgccgccaa cgttgaacag ggcgatgacg ggcgaggtat
    54961 cgatccggag agcctcaccc tccggcacat ccgcaacggg acacgcataa attcgattca
    55021 tcagaacagt acactcaggt tatgggaagt gattaccgct tgatccagga cgatatcccg
    55081 ttccagcaag cgccagttgt catcttccag acggcgaact ttgtcgaaac gcgttccaac
    55141 atagaatgtt tcatcgcgct ctttttgtgc ccgataaagc agataattta cccgcacagc
    55201 aaatacgttt gggatgtcgg tttcgcttat ctggcagttg ctgattaagt gacgggtgcg
    55261 tgacggcggc tcttctgccc aggccatgcc cgtttccaga cgggcgattc gccgctccag
    55321 ctggtctttg gtgtcattaa aaatccaggt tgtcggtggc tggacgcctt tgcggcggtc
    55381 gcgtgtttgc gcgttaactg tggtgcgcat ggtgtaacga atctcttcgt cgagctgcgc
    55441 cagccagtca cgaaatttcc agtcgtccag taagctggct tcgtgaaaga gaaactggct
    55501 aatgcggtga tgtaactcta gtgaaacttg cgcactcatt tcatcacctc ctgctggtaa
    55561 gcggcggttt tatcgagcac ttcctgccag ctttcgctac tcagaagatc agcccagcgt
    55621 tggtacattc cacgagcggc agtttctgaa aagatatagt tagtaatgcc aggaatgccg
    55681 tcgtcgcggc gcttttcctg accaagcccc atttccagac acagtttgct gttgcgggcg
    55741 cggtggcctt taagcaattt ctggatttca caccagttct ccgagtcatc ctgctcgaga
    55801 aaaccagcag gaccaaaagc acgagtggcg ctgttttcaa aagcggcttt aacttcatcg
    55861 gaggcggctt tgtcagtaat acagaacgcc cacacttcaa cttgatcagg gccgcgcgga
    55921 tgccagacgc ggagtgtggc agtgccgttt agccatgaaa gcgtggggaa tatattgtta
    55981 tgacccgcca ggcgcagggc gcgaacttca cctaaacgtt gttctgcttc ggcataggtt
    56041 tcgcggtaat agcttgaaac tgcgccatcg acccagacat tagcatccgg tttttcagta
    56101 aagaagaaac cgctaccgtg accgtcctga ccaaattgca gcgcatcttt ggcggtttcc
    56161 cacaccgggc gggcggtttg tccatcaccg aggcgcttat cgctgccatc atctttcgcc
    56221 cctaatacct gaacggcaga agcatggctg aacagagcat gatactggtc actggcgaac
    56281 tgctctgccg ggaatttcca gttacagttg atcacccact tttgtacgcc gccgacaatt
    56341 tcggtgccgc cttcgcgacg atccagcatg ccatccagat accaggcaat gtcacccagg
    56401 taatcacgca ggcccggtgc gctggtatcc cagttgccaa aaatcagccc tttataactc
    56461 tccacacaag gaacttcgtt tagtccccag tgggatttac acaacccttg tgggtaggcg
    56521 cgaggttcca gcggtacatc gatcaactcg ccgttaatgc cataagacca gccgtgatac
    56581 ggacaggtaa aggcgcgagt gttgccgcaa tctgcataac tcacacgcat ggcccggtgg
    56641 cggcattggt tgagaaacgc cttaatgctg ccgtctttct gacgcactac gacaaccgca
    56701 tcttccccca tgtaggtgtt aaagaaatca ccaggttttg ggatctggct ttcgtgggcg
    56761 agaaataacc agcaacggcc gaaaatacgt tcaagctcca gttggtaaat atctgggtcg
    56821 gtataaatac gcggagtgac ccgaccattt tgggtatcaa tcagttggta aatgttcaaa
    56881 tctgagggtg tggtcatatt ttttaatcct gaaagataag atgtattgac cttctatttt
    56941 tgtgccataa ggatgttgct ggttggttgc taatatgtga aatagttaat ttctaccagc
    57001 tgagacgttt tttcgataac aaggggaagg tgatggaact acggcattta cgctatttcg
    57061 tcgcagtggc gcaggcactg aactttaccc gtgcggcgga aaaactgcat acctcacagc
    57121 cttcgttaag cagccagatc cgcgatcttg aaaactgtgt cggtgttccg ttactggtga
    57181 gggataagcg caaagtcgcg ttgacggcgg cgggagaatg ttttctccag gacgcgctgg
    57241 caatcctcga acaagcggaa aatgccaaat tacgggcgcg gaaaattgtt caggaagaca
    57301 gacaattaac cattggcttt gtgccatcgg cggaagtgaa tttactgcca aaagtattac
    57361 cgatgtttcg tctcagacag ccagacacct tgattgagct ggtgagttta atcaccacgc
    57421 aacaggagga aaaaattcgc cgtggtgaac tcgacgtcgg cttaatgcgc catcctgttt
    57481 atagcccgga gattgattat ctggagcttt ttgacgaacc attagtggtt gtgttaccgg
    57541 ttgatcaccc tttagcacat gaaaaagaga tcaccgccgc gcaactggat ggcgtgaatt
    57601 tcgtcagtac cgatccggtg tattccggtt cgcttgcgcc gatcgttaaa gcgtggtttg
    57661 cgcaagaaaa cagccagcca aatatcgtcc aggtggcaac gaatattctg gtgaccatga
    57721 atctggtggg catggggctg ggcgtcactt tgatacccgg ttatatgaat aattttaata
    57781 ccggacaggt tgtttttcgt cccattgccg gaaatgtgcc ttccattgct ttactgatgg
    57841 cgtggaagaa aggggagatg aagccggcat tacgcgattt catcgccatt gtgcaggaac
    57901 gtttggcaag cgtaacggca taattacgca ttatttttgc aacatgcgtc gcggatctca
    57961 gttttttagt gcttgtgaac tggcgttaca gacatcggga agcaggctcg aatattctta
    58021 tttttcaact tgcaccaaca ttcgattaac cccagactaa cgcctcctga cgggagggac
    58081 tcatggtttt gcaatccacg cgctggttgg cgctcggcta tttcacatac ttttttagtt
    58141 acggcatttt tctacctttc tggagcgtct ggcttaaagg gattggttta acgccagaaa
    58201 ccatcggcct gttattgggg gcaggtctgg ttgcccgttt cctcgggagt ttgctcatcg
    58261 cgccccgcgt cagcgatcct tcccgcctga tttccgcctt gcgcgtgctg gcactgctga
    58321 cacttctctt tgctgtcgcc ttctgggcgg gggcgcacgt agcgtggctg atgctggtga
    58381 tgattggctt taacctcttt ttctcaccac tggtaccgtt gaccgatgca ctggcgaata
    58441 cgtggcaaaa gcagttcccg cttgattacg gcaaagtgcg actgtggggc tcggtggcgt
    58501 ttgtcattgg ctcggcgctg acgggcaaac tggtcactat gtttgattat cgggtgatcc
    58561 tcgcgctgtt gacgttgggc gtggcatcca tgctgctcgg ctttctcatc cgtccgacga
    58621 ttcagccaca aggggcaagt cgccagcagg agagcaccgg ttggtctgcg tggttggcgc
    58681 tggttcgcca gaactggcgc tttctggcct gcgtttgttt attgcagggg gcacatgcgg
    58741 cctattacgg ttttagcgcc atttactggc aggcagctgg ctactcggcc tcggcggtgg
    58801 ggtatttgtg gtcgctgggc gtggtggcgg aagtcattat ctttgcgctg agtaataaac
    58861 ttttccgccg ttgtagtgca cgcgatatgc tgttgatctc ggcgatttgc ggcgtagtgc
    58921 gctggggcat tatgggagca actacggcgt tgccgtggtt gatagtggtg caaattctgc
    58981 attgcggcac cttcacggtc tgccacctgg ccgccatgcg ttatattgct gctcgccagg
    59041 gtagcgaagt catccgttta caggcggttt actctgccgt cgcgatgggc ggcagtatcg
    59101 ctatcatgac cgttttcgcc ggtttcctgt atcaatatct gggccacggc gtgttctggg
    59161 taatggcgct ggtggcgctg ccggcaatgt ttttgcgccc gaaagttgtt ccctcatgct
    59221 gattcgagca ttttgcggat ctgctcctgt tgatgtgttg tcagcgtcag gtccgtatag
    59281 atcagcggtg gtgaaaagag cggtaatggc atggtataag gcgcaataat cagtgccgcg
    59341 ccacgcggag cgcctgtctg caaaaatgcc tttaccgaca tatgcttaat gttgagcggc
    59401 agtaacgtta gttcgcgtaa ctgctgctca atctgcgctt ctcgctcgct atcattcccg
    59461 gtcagtagaa taatctgttt ttcatgcaga tcgttttcct gcattagcca ggcaccgaaa
    59521 atcaccgcga ccagaccact ttcctcatca gaaagatgga cgccgtattc actttcaaat
    59581 ccggccagcg ccgcgcgggt ggtgcgcacc aggcgtgggt acagtctggc gaactcttcc
    59641 ggcagggtat tatcaatacc gatggcaaac aaactgcgat ttaacgcctg ggcgaggtgg
    59701 gtataaagct gatcacataa cccctgttca tcataaaaac gaacatttcc cagctcacga
    59761 aaatggttta ccagacgttt gatagactga cgcaattgtc tgtctcgctg atgcgcatcc
    59821 cgcagtggat cgggaacccg caacatggaa aaaagtagtg ccataaacag tggctcatca
    59881 ggcggtacag gctggagcgc ccgacgctgc cagtggcgtc caatttcctg cgctacctga
    59941 aattcaaggc aggattccgc ccagcgacgt tggagcggat taaactgagg cgtaattcca
    60001 gcgtggtgct gcagcaaaca atattgcaga tacagacaca ggaaatgaat atcgcgcgat
    60061 tcaaagcgtt tttgcagccg tcgggagcag agattcacta acgcttgtag attggtatcg
    60121 tcgtaaaagt tacgcgcaat tccgcgccgc ttcagttcac tcttcagggc gggggtgaat
    60181 tggctggtaa taaatgacgg acacagacgg aaaccacgtc gtagccagtg aaataaacac
    60241 aaacgttggt taagcactgt accttcaacc cggtagctac cgtcataacc agtcgtcagt
    60301 gtgagttgat gatagcgcag gatctcccgt cctgtttcgc tgatatcaag actggcaata
    60361 tcatcatcca caccattaag ctcgctgaag gttgccatgg tggcggtcaa ccccggctga
    60421 aagagcgtca gcaagatctg gcagcggcgc tggggagccg aaaggacaga tggtggagca
    60481 agcgtaggca tcatctgtag atctcccata agcataattg ataagaatag ctaaagtttg
    60541 ctcagggaaa tggcgaaggg aatgctaatc atcagaaatg ttggcaagcg tcacagaatt
    60601 tttctcatct taaactgttc tgaccagtgg attagcatgt cttaaggtgg ttttgtgaat
    60661 gattatcgtg ctagcgatca tgggttagtt ctgcggattc aacgcgctaa ggataaagtc
    60721 caccatctgt tcgcgataaa catcgccgtg gcgatcggaa aaagcatcaa tgtgttcgcc
    60781 atccgggatt aggatcaacc gttttggctc ttttgccagg ctatacaact tttcgctgtg
    60841 ctgccatggg ataacgtgat cagctttacc gtgaatgagt aaaagcggga tcgggctgac
    60901 gctggcgata taattttcgc cgctgtaact ctcatcaagt aagtagccac tgccggggat
    60961 catctggttg gcgatggttg cataagaggc aaatgtggag tcgaggatca ccgcacgtat
    61021 gccttcacga tcaccctgac caataacagc aagaatattc gccccgccaa tactctgccc
    61081 gaacagcacc agccgttgtg ggtttacatc actgcgatgg cgcaccacat tgatggcgct
    61141 ttgcgtatcg tccagcaatc cggcctggga cggcgtgcct tttgatttac caaacccgcg
    61201 ataatcaaac ataaaaacgt tgaaattacg ctcgggtaac caactgacca gcggccagtg
    61261 ggcggacata tttccggcat tgccgtgagc atgaatgatg gttgcgatgg cgttgtcagc
    61321 agggcccgtc gaagaaggaa taaaccagcc ttgcagacga gtaccgtcct tagccgtaaa
    61381 ttcgacggat tccgccgacc aggggtcggg accgtaaatt ttatcgtcag gataatagaa
    61441 gaagttgatg gcaacgcgcg gcactaaata aacgcagaag gcaacaacaa agagaataaa
    61501 cagaattttg ggaacgcgtt tgttcactgg cagtgccata acctttcctt gcgacttctt
    61561 tatcatccat taatcgggga aagatggtgg gagagtgacg aaagaaagta aagcggtgag
    61621 gaagtatttc agaatagcgc ctgaaaggcg agggcgggtg agtgacatca cccgcctgag
    61681 tcattaacgc ttcagagcgt cgcttaactc gtcacgcatg ttcgccagca tggctttcac
    61741 aacacgtggg ttaccagcaa cgatgttacc ggtcagcata tagttatgac caccggtgaa
    61801 gtcgctgacg atgccgcccg cttcacgaac cagcagctcg cctgccgcga aatcccacgg
    61861 acgcagaccg atttcaaaga aaccgtcaac acgacccgca gcgacgtaag ccagatccag
    61921 cgccgcagaa ccggtacgac ggaagtctgc acattcgttg aacagtttgc cgacgatgtt
    61981 gatgtaggta gtggcgtact gttttgcttt gaacgggaag ccggtcgcca gaatggtacc
    62041 gtcgagatcg cgagcggtgc taccgcgcag acggtagccg ttcagctgtg cgccctgacc
    62101 gcgagtggcg gtgaacagtt cgttacgcat aggatcgtat accacagcaa cttcggtgcg
    62161 gcctttgata cgaacggcga tagataccgc gaagtgcggc agacgtttga taaagttggt
    62221 agtaccatcc agtggatcga taacccattg aacatcctga tcagtacctt caagttcacc
    62281 gctttcttcg gtgatgatgg tgtgctgtgg gtaagattta cgaatcgtgt cgataatcac
    62341 cgcttcggca gctttatcta cgttggtcac gaaatcgtta ctgcctttct ggctcgcttc
    62401 tacagcgtcc ggggtttcat agtttttggc aattaaatta cccgccttgc gcgctgcgcg
    62461 cacggcgatg ttcagcatcg gatgcatcgg tctctctcac tggatgttaa agaacgggaa
    62521 aacggcgcag agtatagcag cgagatcggc atctgtcttc gttttatgat aagatgcgtg
    62581 aataatcttt agatgaagaa agacaatgct gcaaaatatt cgaattgtgc tggtggagac
    62641 gtcacacacc ggcaatatgg gttctgttgc ccgtgccatg aaaacaatgg gattaaccaa
    62701 tctgtggctg gttaatccac tggtgaaacc cgactcccag gcgattgccc tggcagcagg
    62761 ggccagcgat gtgattggta atgctcacat cgtcgatacg ctcgacgaag cgttagccgg
    62821 ttgtagcctg gtggtgggca ccagcgcacg ctcccgcacg ctgccatggc cgatgctcga
    62881 cccgcgcgaa tgcggcctga aaagcgtcgc tgaagcggca aataccccgg tggcgctggt
    62941 gtttggtcgc gagcgcgtcg gcttgaccaa tgaagagttg cagaaatgcc attatcatgt
    63001 cgcgattgcg gctaacccgg aatacagctc gctgaacctg gcgatggcgg ctcaggttat
    63061 tgcctatgaa gtgcgcatgg cctggctggc aactcaggaa aacggcgagc aggtcgagca
    63121 tgaagagacg ccgtatccgc tggtcgatga tctggagcgt ttttatggtc atctggaaca
    63181 aacgctgctg gcaaccggtt ttatccgtga aaaccatccg gggcaggtga tgaataaatt
    63241 gcgccgtctg tttacccgtg cgcgcccgga aagccaggaa ttgaatatcc tgcgcgggat
    63301 tctggcttct attgagcagc agaataaagg taataaggcc tgagggctaa gccagataaa
    63361 tgcacggacg gtcccctcgc cccttcgggg agagggttag ggtgagggga agaggccagc
    63421 gctaatgcga ataattgccc tcaacccgcc catggtctgg aaggacaaca ggtacaaacg
    63481 ccacgataaa aaaatggcac tgaaggttaa atacccgact aaatcagtca agtaaatagt
    63541 tgaccaattt actcgggaat gtcagacttg accctgctat gcaatacccc cacttttaca
    63601 ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa gacatgagac tgacatctaa
    63661 agggcgctat gccgtgaccg caatgcttga cgttgcgctc aactctgaag cgggcccggt
    63721 accgttggct gatatttccg aacgtcaggg aatttccctt tcttatctgg aacaactgtt
    63781 ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt ggaccaggcg gtggttatct
    63841 gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg attagcgccg ttgacgaatc
    63901 tgtagatgcc acccgttgtc agggtaaagg cggctgccag ggcggcgata aatgcctgac
    63961 ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt tttctcaaca acattacttt
    64021 aggcgaactg gttaataacc aggaagtgct ggatgtgtct ggtcgtcagc atactcacga
    64081 cgcgccacgc acccgcatac aagacgcgat cgacgttaag ttacgcgctt aatttaaccg
    64141 tcctgtttcg tgttgcatct gcgttgactg cacatactca ctccggtcac ttacttatgt
    64201 aagctcccgg agattcgtaa gtttgtcaac ttgctgaaac ccgaaacagt taggttaaag
    64261 ataacaagat attagaatca ggccggggcg tatcgcgctc cgtaaacacg gtcgtacatc
    64321 cagccggtag cctggttcct tgcattgagt gatgtacgga gtttatagag caatgaaatt
    64381 accgatttat ctcgactact ccgcaaccac gccggtggac ccgcgtgttg ccgagaaaat
    64441 gatgcagttt atgacgatgg acggaacctt tggtaacccg gcctctcgtt ctcaccgttt
    64501 cggctggcag gctgaagaag cggtagatat cgcccgtaat cagattgccg atctggtcgg
    64561 cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc gaatctgaca acctggcgat
    64621 caaaggtgca gccaactttt atcagaaaaa aggcaagcac atcatcacca gcaaaaccga
    64681 acacaaagcg gtactggata cctgccgtca gctggagcgc gaaggttttg aagtcaccta
    64741 cctggcaccg cagcgtaacg gcattatcga cctgaaagaa cttgaagcag cgatgcgtga
    64801 cgacaccatc cttgtttcca tcatgcacgt aaataacgaa atcggcgtgg tgcaggatat
    64861 cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc tatcacgttg atgcaaccca
    64921 gagcgtgggc aaactgccta tcgacctgag ccagttgaaa gttgacctga tgtctttctc
    64981 cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg tatgtacgtc gtaaaccgcg
    65041 cgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag cgcggtatgc gttccggcac
    65101 tctgcctgtt caccagatcg tcggaatggg cgaggcctat cgcatcgcaa aagaagagat
    65161 ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt ctgtggaacg gcatcaaaga
    65221 tatcgaagaa gtttacctga acggtgacct ggaacacggt gcgccgaaca ttctcaacgt
    65281 cagcttcaac tacgttgaag gtgagtcgct gattatggcg ctgaaagacc tcgcagtttc
    65341 ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc tacgtgctgc gcgcgctggg
    65401 gctgaacgac gagctggcac atagctctat ccgtttctct ttaggtcgtt ttactactga
    65461 agaagagatc gactacacca tcgagttagt tcgtaaatcc atcggtcgtc tgcgtgacct
    65521 ttctccgctg tgggaaatgt acaagcaggg cgtggatctg aacagcatcg aatgggctca
    65581 tcattaatcg gtatcggaat caggagaatt tataatggct tacagcgaaa aagttatcga
    65641 ccattacgag aatccgcgta acgtgggttc ctttgacaac aacgacgaga acgtcggcag
    65701 cggcatggtg ggtgcaccgg cctgtggcga cgtgatgaag ttgcagatta aagtcaacga
    65761 tgaaggtatc attgaagacg cgcgttttaa aacttacggc tgcggttctg ctatcgcttc
    65821 cagctccctg gtcaccgaat gggtgaaagg gaagtctctc gacgaagcgc aggcgatcaa
    65881 aaataccgat attgctgaag aacttgaact gccgccggtg aaaattcact gttctattct
    65941 ggcagaagac gcgatcaaag ccgccattgc ggactataaa agcaaacgtg aagcaaaata
    66001 agagttgagg tttggttatg tcgattacac tgagcgacag tgcagcagcg cgagtaaata
    66061 cctttctggc taaccgcggc aaagggtttg gcctgcgtct gggcgtgaga acctccggat
    66121 gttcaggtat ggcttatgta ctggaatttg ttgacgaacc gacgccggaa gacatcgtgt
    66181 ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag cctgcaattt ctggacggta
    66241 cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt taaattcacc aacccgaacg
    66301 ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg atgcgcatac cgataacccc
    66361 accgtggtcg cctgcgcgtg gggtttgttt tacctgattc gccgttatcg cggcggatcg
    66421 cagccctgag aatgttatgg attacttcac cctctttggc ttgcctgccc gttatcaact
    66481 cgatacccag gcgctgagcc tgcgttttca ggatctacaa cgtcagtatc atcctgataa
    66541 attcgccagc ggaagccagg cggaacaact cgccgccgta cagcaatctg caaccattaa
    66601 ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg gaatatttgc tttctttaca
    66661 cggttttgat ctcgccagcg agcagcatac tgtgcgcgac accgcgttcc tgatggaaca
    66721 gttggagctg cgcgaagagc tggacgagat cgaacaggcg aaagatgaag cgcggctgga
    66781 aagctttatc aaacgtgtga aaaagatgtt tgatacccgc catcagttga tggttgaaca
    66841 gttagacaac gaggcgtggg acgcggcggc ggataccgtg cgtaagctgc gttttctcga
    66901 taaactgcgg agcagtgccg aacaactcga agaaaaactg ctcgattttt aatttctgga
    66961 agctaaacat ggccttatta caaattagtg aacctggttt gagtgctgcg ccgcatcagc
    67021 gtcgtctggc ggctggtatt gacctgggca caaccaactc gctggtggcg acagtgcgca
    67081 gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca cctgctgcca tctgttgttc
    67141 actatcaaca gcaagggcat tcggtgggtt atgacgcgcg tactaacgca gcgcttgaca
    67201 ccgccaacac cattagttct gttaaacgcc tgatggggcg ctcgctggct gatatccagc
    67261 aacgctatcc gcatctgcct tatcaattcc aggccagcga aaacggcctg ccgatgattg
    67321 aaacggcggc ggggctgctg aacccggtgc gcgtttctgc ggacatcctc aaagcactgg
    67381 cggcgcgggc aactgaagcc ctggcaggcg agctggatgg tgtagttatc accgttccgg
    67441 cgtactttga cgatgcccag cgtcagggca ccaaagacgc ggcgcgtctg gcgggccttc
    67501 acgtcctgcg cttacttaac gaaccgaccg ctgcggctat cgcctacggg ctggattccg
    67561 gtcaggaagg cgtgatcgcc gtttatgacc tcggcggcgg gacgtttgat atttccattc
    67621 tgcgcttaag tcgcggtgtg tttgaagtgc tggcgaccgg cggtgattcc gcgctcggtg
    67681 gtgatgattt cgaccatctg ctggcggatt acattcgcga gcaggcgggc attcctgatc
    67741 gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc cattgcagcc aaaatcgcgc
    67801 tgagcgatgc ggactccgtg accgttaacg ttgcgggctg gcagggcgaa atcagccgtg
    67861 aacaattcaa tgaactgatc gcgccactgg taaaacgaac cttactggct tgtcgtcgcg
    67921 cgcttaaaga cgcgggtgta gaagctgacg aagtgctgga agtggtgatg gtgggcggtt
    67981 ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt tttcggtcgt ccaccgctga
    68041 cttccatcga cccggataaa gtcgtcgcta ttggcgcggc gattcaggcg gatattctgg
    68101 tgggtaacaa gccagacagc gaaatgctgt tgcttgatgt gatcccactg tcgctgggcc
    68161 tcgaaacgat gggcggcctg gtggagaaag tgattccgcg taataccact attccggtgg
    68221 cccgcgctca ggatttcacc acctttaaag atggtcagac ggcgatgtct atccatgtaa
    68281 tgcagggtga gcgcgaactg gtgcaggact gccgctcact ggcgcgtttt gcgctgcgtg
    68341 gtattccggc gctaccggct ggcggtgcgc atattcgcgt gacgttccag gtcgatgccg
    68401 acggtctttt gagcgtgacg gcgatggaga aatccaccgg cgttgaggcg tctattcagg
    68461 tcaaaccgtc ttacggtctg accgatagcg aaatcgcttc gatgatcaaa gactcaatga
    68521 gctatgccga gcaggacgta aaagcccgaa tgctggcaga acaaaaagta gaagcggcgc
    68581 gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc cgcgctgtta agcgccgcag
    68641 aacgtcaggt cattgacgat gctgccgctc acctgagtga agtggcgcag ggcgatgacg
    68701 ttgacgccat cgaacaagcg attaaaaacg tagacaaaca aacccaggat ttcgccgctc
    68761 gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca ttccgtggac gaggtttaat
    68821 atgccaaaga ttgttatttt gcctcatcag gatctctgcc ctgatggcgc tgttctggaa
    68881 gctaatagcg gtgaaaccat tctcgacgca gctctgcgta acggtatcga gattgaacac
    68941 gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca tcgttcgtga aggttttgac
    69001 tcgctgccgg aaagctcaga gcaggaagac gacatgctgg acaaagcctg gggactggag
    69061 ccggaaagcc gtttaagctg ccaggcgcgc gtcaccgacg aagatttagt ggtcgaaatc
    69121 ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga ggttagtatg ggacttaaat
    69181 ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc gtatcccgat cttgatccga
    69241 aaacggttcg attcaccgat atgcatcagt ggatttgcga tctggaagat ttcgacgacg
    69301 acccgcaggc atccaacgag aaaatcctcg aagcgatttt gttagtctgg ctggacgagg
    69361 ccgaataatt tccgcgtcat gcttcacgcc gcagatgcgt tggctgcgct cgttcacccc
    69421 agtcacatag ttatctatgc tcctggggct tcactcactt gccgccttcc tgcaacgcga
    69481 atcatttagc ggaaaaatcc tggggctgcc aactggcggc ccttttacaa agaaggataa
    69541 ctaaaatgac agaagcgatg aagattaccc tctctaccca acctgccgac gcgcgctggg
    69601 gagaaaaagc aacttacagc attaataatg acggcattac cctgcatttg aacggggcag
    69661 acgatctggg gctgatccag cgtgcggcgc gcaagattga cggtctgggc atcaagcatg
    69721 ttcagttaag cggtgaaggc tgggatgcgg atcgctgctg ggcattctgg caaggttaca
    69781 aagccccgaa aggcacgcgt aaagtggagt ggccggatct ggacgatgcc cagcgccagg
    69841 aactggataa ccgcctgatg atcatcgact gggtgcgtga caccatcaac gcaccggcag
    69901 aagaattggg accatcgcaa ctggcacagc gtgctgttga tctgatcagc aacgtcgcgg
    69961 gcgatcgtgt gacttatcgg atcaccaaag gcgaagatct gcgtgagcaa ggttatatgg
    70021 ggctgcacac cgtcgggcgc ggttcagaac gttctccggt attgctggcg ctggattaca
    70081 acccaactgg cgataaagaa gcaccggtgt acgcgtgcct ggtaggtaaa ggtatcactt
    70141 ttgactccgg cggctacagc atcaaacaga ctgcgtttat ggactcgatg aagtcggaca
    70201 tgggcggcgc ggcaacggtt accggggcgc tggcatttgc cattacgcgc ggactgaaca
    70261 agcgcgtgaa gctgttcctc tgctgtgcgg ataacctgat tagcggcaat gcgttcaagc
    70321 tgggcgatat catcacctat cgcaacggta aaaaagttga agtgatgaac actgatgccg
    70381 aagggcgtct ggtgcttgcc gatggtctga ttgatgccag tgcgcagaaa ccggaaatga
    70441 tcattgatgc ggcgaccctc accggggcgg cgaaaactgc gctgggtaat gattatcacg
    70501 cgctgttcag ttttgacgat gcgcttgccg gtcgtttgct ggcgagtgcc tcacaagaga
    70561 acgaaccatt ctggcgtctg ccgctggcgg aattccaccg cagccagctg ccgtctaact
    70621 ttgccgaact gaacaatacc ggaagcgcgg cgtatccggc aggcgcgagc acggcggcgg
    70681 gcttcctgtc gcactttgtt gagaactatc agcaaggctg gctgcatatc gactgctcgg
    70741 cgacttaccg taaagcgccg gttgaacagt ggtctgcggg cgctacggga cttggtgtgc
    70801 gcacgatagc taatctgtta acggcgtaaa ctaatatgcc ggatgcggtg taaacgcctt
    70861 atccggccta caaattctgc aaattcaaca aattgcgaat cccttgtagg cctgataagc
    70921 gtagcgcatc aggcatttgc gccccgaacg ttgaatgaat tgtggttcat atgtccgaaa
    70981 caaaaaacga acttgaagac ctgctggaaa aagcagcaac cgaaccggcg caccgcccgg
    71041 gctttttccg tactctactg gaatccaccg tctgggtgcc tggtacggcg gcgcagggcg
    71101 aggctgtggt tgaagatagt gcgcttgatt tacagcactg ggaaaaagaa gacggcacca
    71161 gcgtcattcc ttttttcacc tcgttagaag cgcttcaaca ggcggttgaa gacgaacagg
    71221 catttgtcgt aatgcccgtt cgcacattgt ttgagatgac ccttggcgaa acgctcttcc
    71281 ttaatgccaa actgccgacc ggtaaagaat ttatgccgcg tgaaatcagt ttgttgattg
    71341 gtggagaggg aaatccgctg agcagccagg aaatcctgga aggcggtgaa tcgctgatat
    71401 tatcggaagt cgcagagccg ccagcacaaa tgattgattc actcaccacc ttatttaaaa
    71461 ccattaagcc ggtgaagcgt gcttttattt gttcaattaa agagaacgaa gaggcacagc
    71521 ctaatttact tattggcatt gaagccgatg gtgatatcga agaaattatt caggcgacgg
    71581 gaagtgtagc gaccgataca ttacctggcg atgaaccaat cgatatttgt caggtgaaaa
    71641 aaggggaaaa aggaattagc cactttatta ccgaacatat tgcgccattc tatgaacgtc
    71701 gctggggtgg ttttttgcgt gactttaaac agaaccggat aatctaaaat atgccgccct
    71761 aaagggcggc attcgggctt acttgctgct ctgaaggttg ttaaccacca gtaaattgtc
    71821 gccggagaga ctccggttat tatttacttg ggaaaacagc gcaccagaaa caagaagtac
    71881 gatacagaag caccgtatct tcattgtgcg gtccatgcga agaccgcatc acgacagcac
    71941 cactggcgat attgccgcga tagcaagcgc tgagacgcgc ttgctctaac ggtcgcggaa
    72001 catcctttcc ttaagccagc gcccgcttgc gagacggcgg aaagggccgc agtggataat
    72061 acactaacag aacaataata actctctttt gcttgacaaa aagagagtta ctggcgagta
    72121 ttgtctccgc tgagacgcgc ttgcattact acggtaatac aacctgctgg aaaggccgcg
    72181 aaccagacca gcaataaaaa accgccaaat tcggcggttt tttattgcct gtaaaatact
    72241 tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg
    72301 tacagtttca cgtttggcac atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt
    72361 acaccagagc cgcagctgac gataattggt ttgtcgtagc tgacgccgcg accaaaaaat
    72421 atcgcatcca gttcatcggt cgtttttagt tcgccttcgc gcaccagttc cgtccacgga
    72481 acattcagtg ctccgggaat atgtccgcga cgtaaacctg ggcgaggttc atcaacttct
    72541 gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg ccgtattttc atggcttgcc
    72601 aataatacat cggttacttt caccacggct tcaggattaa acgcggcgtt aaactctcct
    72661 tccggcagct ctactgcacc ttcttctaac agcagatcat cgcgctgcca gcctgcaagt
    72721 ccacccccca gaatcgacac tttctctaca ccaaaggtgc gcagcatcca ccatgctcgt
    72781 ggggctgaga aaagattacc ttcgtcatag acaatcaggt gcttatcctg gttaacgcct
    72841 aattcacgca tcgccacggc gaacgtttcc gggcgcggca gcatgtgcgg aagcggggaa
    72901 gtgtgatcag aaagcgcttc gatatcaaaa aacactgcgc cgggaatatg tccattcaga
    72961 tactcctgag caacgttacg atcttcctgt ccaggcgacg ccatgcgggc atcaataatc
    73021 tgaatttccg ggtcatcaat atgttcggcg agccagtcgg ctcctacaaa ccatgtcgtg
    73081 gacataggca tctccatttt tgccggggtt aggctaaatt gtcggatgtt tagtgaaaag
    73141 cgacaagcga aagaggctga tttggctgtc tgagccacaa ttctcactaa aattcggatt
    73201 gtatgggcgt gatactgaat tacataacgt aaattctcac gcataataat tattcgacgt
    73261 tgctaacggg ccattgtggc agggatgaaa aatgaaaaag ttacgcgtag ccgcctgcat
    73321 gctaatgctg gcgctggcag ggtgcgacaa caacgataac gcgccaacag cggtgaaaaa
    73381 agatgcgcct tctgaagtta ctaaagcggc ctcttcagaa aacgcgagtt cagcaaaact
    73441 ctccgtgccg gagagacaaa aactggccca acagagtgcc ggtaaggtgc tgacattgct
    73501 ggatctctct gaagtccaac ttgatggtgc agccacgctg gtgctgacgt tctccatccc
    73561 tctcgacccg gatcaggatt tctcacgcgt tattcatgtc gtcgataaaa aaagcggcaa
    73621 agtggatggt gcctgggagc tgtcagataa tcttaaagag ttacgtttac gccacctcga
    73681 accgaaacgt gatttgatcg ttactattgg caaggaggtc aaagcactca acaacgcaac
    73741 cttcagtaaa gattacgaaa aaactataac tacccgcgac atccaaccca gcgtcggttt
    73801 tgccagccgt ggttcgctgc tgcctggcaa agtcgttgaa gggctgccgg taatggcgct
    73861 caacgttaat aatgtcgatg ttaacttctt ccgcgttaag ccagaatctc tgccagcatt
    73921 cattagccaa tgggaatacc gcaattcgct ggcgaactgg cagtcagaca aactgctgca
    73981 gatggcggat ctggtctaca ccggacggtt tgatctcaat cctgcgcgta acacccgtga
    74041 aaaattattg ctgccgctgg gcgatatcaa accgcttcag caggcgggcg tgtatctggc
    74101 tgtgatgaat caggctggac gttacgatta cagtaatccc gcgacgctgt ttacgttaag
    74161 tgatatcggc gtttcagctc accgttatca caatcgtctg gatatcttta cccaaagtct
    74221 ggaaaacggc gcggcccagc aaggaattga agtctcttta ttaaatgaga aagggcagac
    74281 tctgactcag gcaaccagtg acgctcaggg gcatgtgcag ctggaaaatg ataaaaacgc
    74341 ggcattactg ttggcgcgta aagacggtca gacaacgcta ctcgatttaa aacttccggc
    74401 gctggactta gcagaattta acattgctgg cgcgccaggc tatagcaaac agtttttcat
    74461 gtttggccca cgcgatcttt atcgcccaag tgaaacggta atcctcaatg gtttgctgcg
    74521 tgatgcagac ggtaaagcgt tgcccaatca acccatcaag ttagacgtga ttaaacccga
    74581 tgggcaggta ctcaggagcg tcgttagtca gccggagaat ggcctctacc actttacctg
    74641 gccactcgat agcaatgcgg caaccggtat gtggcatatt cgcgctaaca cgggcgataa
    74701 ccagtatcgg atgtgggatt tccacgtcga agattttatg ccagagcgca tggcgctgaa
    74761 tctgaccggt gagaaaaccc cgctaacgcc gaaagatgaa gtgaaattct ccgtggtggg
    74821 gtactacctg tatggtgcac ctgctaatgg taatactttg caagggcaac ttttcctgcg
    74881 cccactgcgt gaagctgtgt cagccttacc tggttttgaa ttcggcgata tagctgccga
    74941 aaatctttcc cgcacgctgg atgaagttca gttgacgctg gatgataaag ggcgcggcga
    75001 agtttctaca gaaagccagt ggaaggaaac gcattcccca ttacaggtta ttttccaggg
    75061 tagtttgctg gaatcgggcg gtcgcccggt gacgcgccgc gctgagcagg ctatctggcc
    75121 tgccgatgca ttgccgggga tccgtccgca gttcgcctcg aaatcggttt acgattatcg
    75181 tactgacagc acggtgaaac agcccattgt tgatgaaggc agtaacgccg cttttgacat
    75241 cgtttatagc gatgcgcaag gcgtgaaaaa agccgtgtcg ggcttgcagg tgcgcctgat
    75301 tcgcgaacgc cgcgattact actggaactg gtcagaagat gaaggctggc agtcacagtt
    75361 tgatcaaaaa gatctgatcg aaaatgaaca aactctggat ctgaaagcgg acgaaaccgg
    75421 caaggtcagt tttccggtag agtggggcgc ttatcgtctg gaagtcaaag cgccgaatga
    75481 agcggtcagt agtgttcgtt tctgggctgg ctatagctgg caggacaaca gcgacggtag
    75541 cggcgcagtg cgacccgacc gtgtcacgct gaaactggat aaagccagtt atcgccctgg
    75601 cgacaccatt aagttgcata tcgccgcgcc aacggcgggt aaaggttatg cgatggtcga
    75661 gtccagtgaa gggccgctgt ggtggcaaga gattgatgtt cgggctcaag ggctggatct
    75721 gacgattccg gtcgataaaa cctggaatcg tcatgatctg tatttaagta cgctggtggt
    75781 acgtcctggc gataaatctc gctccgcgac gccaaaacgc gcggttggtg tgttgcatct
    75841 gccgcttggc gatgaaaacc gtcgcctcga tctggcgctg gaaacaccag caaaaatgcg
    75901 tcccaatcaa ccattaaccg tgaaaattaa agccagcact aaaaatggcg agaagcctaa
    75961 acaggtgaat gtgctggtgt ctgccgttga tagtggtgtg ctgaatatta ctgactacgt
    76021 cacgccagat ccgtggcagg cgttctttgg tcagaaacgc tatggcgcag acatttacga
    76081 tatttacggt caggttattg aaggtcaggg gcgtctggca gctctgcgtt tcggtggcga
    76141 tggtgatgag ctgaaacgtg gtggtaaacc gccggtcaat cacgtcaata ttgtcgcgca
    76201 gcaggcgctg ccggtaacgc tcaacgaaca gggcgaaggc tcggttacac tgccgattgg
    76261 cgattttaac ggtgaattgc gcgtcatggc gcaagcctgg acggcagatg atttcggtag
    76321 caacgaaagc aaagtgatag ttgccgcacc ggtgattgct gaactgaaca tgccgcgctt
    76381 tatggcgagt ggcgatacct cgcgtctgac gctggatatc actaatctta ccgataaacc
    76441 gcaaaaactg aacgttgccc tgaccgccag tggtttgctt gaactggtca gcgattcacc
    76501 cgcagccgtt gaattagcgc caggtgtgcg tactacgctg tttatcccgg tgcgagcatt
    76561 gccgggttat ggcgatggag aaattcaggc caccattagc gggttagcgt taccgggtga
    76621 aaccgttgcc gatcagcata agcagtggaa aatcggcgtc cgtccggcgt tcccggcaca
    76681 aacggttaat tacggtacgg cgttacagcc tggtgagaca tgggcgattc cggcggatgg
    76741 attgcaaaac ttctcgcctg ttacgctgga agggcaattg ttgttgagcg gcaaaccacc
    76801 gctgaacatt gcacgttata tcaaagagtt aaaagcgtat ccgtacggct gtcttgagca
    76861 aaccgccagc ggcctgttcc cgtcacttta taccaacgca gcccaactgc aggcgttggg
    76921 catcaaaggc gacagtgatg agaaacgccg tgcatcggtc gatatcggca tttcccgttt
    76981 gctgcaaatg caacgtgata acggcggctt tgcgctgtgg gataaaaacg gtgacgaaga
    77041 gtactggctg acggcttacg tgatggattt cctggtccgc gcaggcgaac agggttacag
    77101 cgtgccgaca gacgccatta accggggtaa tgagcgtctg ctgcgctatt tacaagatcc
    77161 gggcatgatg tcgatcccgt acgcggataa tctcaaagcc agtaaattcg ccgttcagtc
    77221 ttacgctgcg ctggtgctgg cccgtcagca aaaagcacca ctgggtgcgc tgcgtgaaat
    77281 ctgggagcat cgtgcagatg ccgcttctgg tttaccgctg ctgcaacttg gcgttgcgct
    77341 gaaaaccatg ggtgatgcaa cgcgtggtga agaagcgatt gtgctggcgc tgaaaacgcc
    77401 gagaaatagt gatgagcgga tatggctggg tgattacggt agtccactgc gcgacaacgc
    77461 gttaatgctc tccttgctgg aagaaaataa actgctaccc gatgagcaga acactttgct
    77521 gaacacgctt tcgcagcagg catttggtga acgctggcta tcgacgcagg aaagtaacgc
    77581 gttgttcctg gctgcccgta cgattcagga tttaccgggt aaatggcagg cgcaaacctc
    77641 tttctcagct gagccgctga caggcgagaa aacgctaaac agcaatctga atagcgatca
    77701 acttgccacc ttgcaggtga gaaacagtgg cgatcagccg ttatggctgc gtgtggatgc
    77761 cagcggttat ccgcaatccg cacctttacc ggcgaacgat gtgctgcaaa tcgagcgtca
    77821 tattcttggt actgatggta agagcaaatc gctggactcg ttacgtagcg gcgatctggt
    77881 gctggtgtgg ttgcaggtaa aagccagtaa cagcgtgccg gatgcgttgg tcgtggatct
    77941 gctgcctgcg ggtctggaac tggaaaacca gaatctggcg aacggtagcg ccagcctgga
    78001 gcaaagtggt ggcgaagtgc agaacttact gaaccagatg cagcaggcga gtattaagca
    78061 cattgagttc cgtgacgatc gctttgtggc ggcggttgcc gttgatgaat accaaccggt
    78121 aacgctggtg tatctggcgc gggcggtgac gccgggaacg tatcaggtac cgcaaccgat
    78181 ggtggaatca atgtatgttc cccaatggcg ggcgaccggc gcggctgaag atctgctgat
    78241 tgtccgaccg taaatgcctc gcttgttaac caaacgcggc tgctggataa cgctggcagc
    78301 tgcgccattt ctcctttttc ttgcagcgtg gggagcagat aaactctggc ctctaccgct
    78361 gcatgaagtc aatcccgcac gagtggtcgt ggcgcaggat ggtacgccgc tatggcgctt
    78421 cgccgatgct gacggcatct ggcgttatcc ggtaacaatc gaagatgttt ctccacgtta
    78481 ccttgaagcg ctgatcaatt atgaagatcg ctggttctgg aagcatccgg gggtgaatcc
    78541 attctcggtg gcgcgcgcag catggcaaga tctcacttcg ggacgggtta tttccggtgg
    78601 cagcacgctc actatgcagg ttgctcgtct gcttgatcct caccccaaaa catttggcgg
    78661 caaaattcgc cagctctggc gcgcgttgca actggaatgg catctgtcta agcgtgaaat
    78721 tctgaccttg tatcttaacc gcgctccgtt tggcggtacg ttgcagggga tcggtgcggc
    78781 aagttgggct tatctcggaa agtcgcctgc gaatttaagc tattccgagg cggcaatgct
    78841 ggcggttttg ccgcaagcgc ccagccgtct tcgcccggat cgttggccgg agcgtgccga
    78901 agccgcgcgt aataaagtgc tcgaacggat ggccgtgcaa ggtgcgtggt cccgtgagca
    78961 ggtaaaagag tcaagggaag aacccatctg gctggcaccc cgacaaatgc cgcaactggc
    79021 accgctgttt tcgcgcatga tgctcggtaa aagcaaaagc gacaaaatcg ttactacgct
    79081 ggatgccggt cttcaacgac gtctggaaga actggcgcaa aactggaaag ggcggttgcc
    79141 accgcgcagc tcactggcga tgatcgtggt tgatcatacc gatatgcgtg ttcgcggctg
    79201 ggtgggatcg gttgatctca acgatgattc acgctttggt catgttgata tggtcaatgc
    79261 gaaccgatcg ccaggatcgg tgctcaaacc gtttgtttat ggtctggcgc tggatgaagg
    79321 cttgatccac ccggcatcac tgctgcaaga cgtcccccgg cgcaccggtg attatcgacc
    79381 aggtaacttt gatagcggtt ttcatggccc gatcagcatg agcgaggcgc tggtgcgctc
    79441 gctgaactta cctgctgtgc aggtgctgga agcctatgga ccgaaacggt ttgcggcaaa
    79501 gttacgcaat gttggattgc cgttatattt gcccaacggt gctgcgccga atctttcact
    79561 cattctcggc ggcgctggtg caaaactgga agatatggcg gcagcgtata ccgcgtttgc
    79621 tcgccacggc aaggcaggca aattgcgctt acagcctgac gatccgctgc ttgaacggcc
    79681 tttaatgtcg tcgggggcgg cgtggatcat tcgccggatt atggctgatg aagcgaaacc
    79741 cttgccggat agtgccttgc cgcgcgtcgc cccactggca tggaaaacgg gcaccagcta
    79801 tggctatcgt gacgcctggg cgattggggt taacgctcgc tatgtcattg ggatctggac
    79861 tggcagaccg gacggcacgc ccgttgttgg tcagtttggc tttgccagtg ccgtaccatt
    79921 gttgaatcag gtcaataata tcttactgtc gcgcagtgcg aatctgccgg aagacccgcg
    79981 cccgaactca gtcactcgtg gcgttatctg ctggccgggc ggacagtctt tgccggaagg
    80041 tgacggtaac tgtcgccgcc gcctggcaac ctggctgctg gacgggagtc agccgccaac
    80101 tctattactg ccggagcagg aaggcattaa tggcattcgt ttccccatct ggctggatga
    80161 aaatggcaaa cgtgttgccg ctgattgccc gcaagcgcga caagaaatga taaatgtctg
    80221 gccgctaccg ctggaaccct ggctgcccgc atcagaacgt cgcgctgtgc gcttaccacc
    80281 ggcctcgaca atttgcccgt catacggtca cgatgcccag ctccctctgc agctgacagg
    80341 cgttcgcgat ggcgcgatta ttaaacgttt accgggcgca gcggaagcga ctttgccgtt
    80401 gcaatcaagt ggaggggcag gtgaacgctg gtggtttctg aatggcgaac cgttaactga
    80461 acgcgggcgc aacgtgaccc tgcatttgac ggataaaggt gattatcaat tgctggtgat
    80521 ggatgatgtg gggcaaatcg cgacagtgaa atttgtcatg caatagtctg ttttgtccct
    80581 gattgttgct aaaactcatt ttattttaaa aaaatgttac cttcatcaat agtcaacggc
    80641 cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca acagaacaat
    80701 ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg aacgcggtag
    80761 caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc aaaattgtcg
    80821 gcaccaaaat gctgcacctg accgttgaac aggcacgtgg cttttatgct gaacacgatg
    80881 gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc gtggtttccg
    80941 tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg accaatccgg
    81001 caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc gaaaacggta
    81061 cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc tttggcgaag
    81121 gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa acgtggcatc
    81181 cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca ccggggcgtt
    81241 tcttttttca atcctccagg ggccataacg tgtaataacg aggccggaat agcatatgtc
    81301 tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca acctgctgga
    81361 tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaac ccttccgcgc
    81421 cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg agatgaccga
    81481 catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg caccggaagt
    81541 ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg ttggcgatca
    81601 gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg tctcttcgca
    81661 ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct ttaaccgcaa
    81721 cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg tcggcgcagc
    81781 aaaagtcacc ggtcagcgcc caatcactaa cgtagtgatg atgggcatgg gcgagccgct
    81841 gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt tcggttttgg
    81901 cctgtctaaa cgtcgcgtca cgctttctac ttccggtgta gttccggcgc tggataaact
    81961 gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg acgaaattcg
    82021 tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg ctgcggtgcg
    82081 ccgttatctg gagaaatcca acgccaatca gggccgtgtc actattgagt atgtgatgct
    82141 tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc tgaaagatac
    82201 gccgtgtaag atcaacctga tcccgtggaa ccccttcccg ggcgcgccgt atggacgcag
    82261 ctcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt tcaccactat
    82321 tgtgcgtaaa acccgtggtg atgatatcga tgctgcctgt ggtcagttgg cgggcgatgt
    82381 tatcgaccgt acgaaacgta ccctgcgtaa acggatgcag ggtgaagcca tcgacattaa
    82441 agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa tcttttatga
    82501 atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg ctgactttaa
    82561 ggcagtatgt aacgatgcaa cagtaagtta gccttagtga atgtgggctt tgtcacgagc
    82621 acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc gcggtgtggg
    82681 tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca gcgaatgaat
    82741 actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct gcgtaatgct
    82801 cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct gaaggtttcc
    82861 acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac attcctgcgc
    82921 ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact gctgccaggg
    82981 ctggaaaagc aggctccact acgggctgca aaagttgcgc ctatgcagag tttttccctc
    83041 ggtaaacgcc gcaaaaaacg tgacggctgg ctgatgacct tcacctggct ggtgttgttt
    83101 gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca gcaggaagag
    83161 atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga gcaggggcag
    83221 agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac gccgccagcg
    83281 tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc agcaccagct
    83341 gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga taccgccgcg
    83401 accccggcac caacggcaac aacaacgcca gatggcgctg cgccgttgcc aaccgatcag
    83461 gcaggcgtga ctacgccggc ggctgatccg aatgcgctgg tgatgaactt tactgccgat
    83521 tgctggctgg aggttactga tgctaccggt aaaaaattgt ttagcggtat gcagcgtaaa
    83581 gacggtaatt tgaacttaac cggccaggca ccatacaaac tgaaaattgg tgcgccagcc
    83641 gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat cagaactaac
    83701 caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca gacgggtaac
    83761 gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca acacgtattt
    83821 acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc atgaccaata
    83881 cgcgtacgac agacgtcgaa gcaacggtca atcaaatcaa ggcgctggaa cgcgttggcg
    83941 ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc aaactcatca
    84001 aacagcaggt taacgtgccg ctggtggctg acatccactt cgactatcgc attgcactga
    84061 aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggcaatatc ggtaatgaag
    84121 agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc cgtattggcg
    84181 ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg acgccgcagg
    84241 cgttgctgga atccgccatg cgccatgttg atcatctcga tcgcctgaac ttcgatcagt
    84301 tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat cgtttgctgg
    84361 caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt gcacgcagcg
    84421 gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc tgaaggcatc ggcgacacgc
    84481 tgcgcgtatc actggcggcc gatccggtcg aagagatcaa agtcggtttc gatattttga
    84541 aatcgctgcg tatccgttcg cgcgggatca acttcatcgc ctgcccgacc tgttcgcgtc
    84601 aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa gatatcatca
    84661 ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag gcgctggttt
    84721 ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat ggcgtgcgca
    84781 aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt cgtgcgaaag
    84841 ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttgaaaaa taataacgtg
    84901 atgggaagcg cctcgcttcc cgtgtatgat tgaacccgca tagctcccga aacattgagg
    84961 gaagcgttga gggttcattt ttatattcag aaagagaata aacgtggcaa aaaacattca
    85021 agccattcgc ggcatgaacg attacctgcc tggcgaaacg gccatctggc agcgcattga
    85081 aggcacactg aaaaacgtgc tcggcagcta cggttacagt gaaatccgct tgccgattgt
    85141 agagcagacc ccgctattca aacgtgcgat tggtgaagtc accgacgtgg ttgaaaaaga
    85201 gatgtacacc tttgaggatc gcaatggcga cagcctgact ctgcgccctg aagggacggc
    85261 gggctgtgta cgcgccggca tcgagcatgg tcttctgtac aatcaggaac agcgtctgtg
    85321 gtatatcggg ccgatgttcc gtcacgagcg tccgcagaaa gggcgttatc gtcagttcca
    85381 tcagttgggc tgcgaagttt tcggtctgca aggtccggat atcgacgctg aactgattat
    85441 gctcaccgcc cgctggtggc gcgcgctggg tatttccgaa cacgtaactc ttgagctgaa
    85501 ttctatcggt tcgctggaag cacgcgccaa ttaccgcgat gcgctggtgg cattccttga
    85561 gcagcataaa gaaaagctgg acgaagactg caaacgccgc atgtacacta acccgctgcg
    85621 cgtgctggat tcaaaaaatc cggaagtgca ggcgcttctc aacgacgctc cggcattagg
    85681 tgattatctg gacgaggaat ctcgtgagca ttttgccggt ctgtgcaaac tgcttgagag
    85741 cgcggggatc gcttacaccg taaaccagcg tctggtgcgt ggtctggatt actataaccg
    85801 taccgttttc gagtgggtga ctaacagtct cggctcccag ggcaccgtgt gtgcaggcgg
    85861 tcgttatgac ggtcttgtgg aacaactggg cggtcgtgca acaccggctg tcggttttgc
    85921 gatgggcctc gaacgtcttg tattgttagt acaggccgtt aatccggaat ttaaagccga
    85981 tcctgttgtc gatatatacc tggtggcttc aggtgctgat acacaatctg cggctatggc
    86041 attagctgag cgtctgcgtg atgaattacc gggcgtgaaa ttgatgacca accacggcgg
    86101 cggcaacttt aagaaacagt ttgcccgtgc tgataaatgg ggtgcccgcg ttgctgtggt
    86161 gctgggtgag tctgaagtgg ctaacggcac agcagtagtg aaggatttgc gctctggtga
    86221 gcaaacggca gttgcgcagg atagcgtagc cgcgcatttg cgcacgttac tgggttaagg
    86281 aaggagaagg acagcgtgga aatttacgag aacgaaaacg accaggtaga agcggttaaa
    86341 cgcttttttg ctgaaaatgg caaagcactg gctgttgggg tgattttggg cgttggcgca
    86401 ctgattggct ggcgctactg gaacagccat caggttgatt ctgcacgctc cgcttctctt
    86461 gcctatcaaa atgcggttac cgcagtgagc gaaggcaaac cggatagcat cccggcggcg
    86521 gaaaaatttg ctgctgaaaa taaaaatact tatggtgcgc tggcttcttt ggaacttgcg
    86581 cagcaatttg ttgacaaaaa tgaactagag aaagctgccg cccagttaca acaggggctg
    86641 gcagacacga gcgatgaaaa tctcaaagcc gtgataaatc tgcgtcttgc tcgcgttcag
    86701 gtacagctca agcaggctga tgccgcgctg aaaacccttg ataccatcaa aggtgaaggg
    86761 tgggctgcca ttgttgccga cctgcgtggt gaagcattgc tgagcaaagg tgataagcaa
    86821 ggtgcgcgta gtgcatggga agcaggcgtg aaaagcgatg ttactccggc actgagcgaa
    86881 atgatgcaga tgaaaattaa taatttgtcc atctgagagg gacccgatgc aattgcgtaa
    86941 attactgctg ccaggactgc tttccgttac ccttttaagc ggctgttcgc tgtttaacag
    87001 cgaagaagat gtggtaaaga tgtccccatt gccaaccgtt gaaaaccagt ttacgccgac
    87061 cacggcgtgg agcacttccg ttggtagcgg cattggcaac ttctattcca atcttcatcc
    87121 ggcactggcg gacaacgttg tctatgcagc ggaccgcgct ggtttggtaa aagcgctgaa
    87181 tgcggatgat ggcaaagaaa tctggtctgt cagcctggcc gagaaagatg gctggttctc
    87241 taaagagcct gcattacttt ctggcggtct gaccgtgtct ggtgggcatg tctacattgg
    87301 cagcgaaaag gcgcaggttt acgcgctgaa taccagcgat ggtactgtgg catggcaaac
    87361 taaagtcgcg ggtgaagcac tttcgcgccc ggtggtcagc gacggtctgg tgttaatcca
    87421 caccagtaac ggtcagttac aagcgctgaa cgaggctgac ggcgctgtca aatggacagt
    87481 taacctcgat atgccttcgc tctctttgcg tggcgagtct gcgccggcaa cggcttttgg
    87541 tgcggccgtc gtgggtggcg ataatggtcg cgtcagcgca gtgctgatgg aacagggcca
    87601 gatgatttgg cagcagcgta tttcccaggc gaccggttct accgaaattg accgtctgag
    87661 cgatgttgac acgactcccg tcgttgttaa cggcgttgtt ttcgcgctgg cctataatgg
    87721 taacctgacg gcgcttgatc tgcgcagtgg tcagattatg tggaaacgcg aactgggttc
    87781 ggtgaatgat ttcatcgtcg acggcaatcg catctatctg gtcgatcaaa atgaccgggt
    87841 gatggcgttg accattgatg gcggcgttac gctgtggaca caaagcgatc tgttgcatcg
    87901 cctgctgact tctccggtgc tgtataatgg caacctggtg gtaggtgaca gtgaaggtta
    87961 tctgcactgg attaacgtcg aagatggtcg tttcgttgcc cagcaaaaag ttgatagttc
    88021 cggtttccag actgaaccgg ttgccgctga cggcaaactg ctgatccagg caaaagacgg
    88081 aaccgtgtac tctattacac gttaatcgtc tctgtcgttc actttgaaaa cggctcctgg
    88141 acaggggccg ttttcctgtt tttaacaacg acgcgaatat agcgtgcgtt gtctgatgat
    88201 ttataaaaat gaggctttaa acatggtacc tgtggtcgcg cttgtcgggc gccctaacgt
    88261 aggaaaatcc acgttattta accgtctaac tcgcacccga gatgcgctgg ttgcggattt
    88321 cccgggtctg actcgtgacc gtaagtacgg tcgtgcggaa attgaaggcc gtgagtttat
    88381 ctgtattgat accggcggga ttgatggcac agaagacggt gtagaaaccc gcatggcgga
    88441 acagtcgctg ctggcgattg aagaagcgga cgtcgtactg tttatggtgg atgcgcgcgc
    88501 gggcctgatg ccggcagatg aagcgattgc caaacatctg cgctcccgtg aaaaaccgac
    88561 cttcctggtg gcaaacaaaa ctgacggtct ggatcccgat caggcagtgg ttgatttcta
    88621 ctcgcttggt ttaggtgaaa tctacccgat cgccgcgtct cacggtcgtg gcgtattaag
    88681 tctgctggag catgtgctgc tgccgtggat ggaagatctc gcaccgcaag aggaagtcga
    88741 cgaagacgct gaatactggg cgcaatttga agcggaagag aacggcgaag aagaagagga
    88801 agacgacttc gacccgcaaa gtctgccgat caaactggcg attgtgggtc gtccgaacgt
    88861 aggtaagtct acactcacta accgtattct tggtgaagag cgcgttgttg tttatgacat
    88921 gcctggcacg acgcgtgaca gtatctacat tccaatggaa cgcgatggac gtgagtatgt
    88981 gctcattgac accgctggcg tacgtaaacg cggcaaaatc accgatgctg tagagaaatt
    89041 ctccgtaatc aaaacgttgc aggccattga agatgccaac gtggtgatgt tagtgattga
    89101 tgcgcgcgaa ggtatttccg atcaggatct ctcgctgctg ggctttattc tcaatagtgg
    89161 gcgctcactt gtcattgtgg tgaataagtg ggatggcctg agccaggaag tgaaagagca
    89221 ggtgaaagaa acgctggact tccgtctggg ctttatcgat tttgctcgtg tgcactttat
    89281 ctctgccttg cacggcagtg gtgttggtaa cttgtttgaa tcagtacgtg aagcgtatga
    89341 cagctccacc cgtcgtgtgg ggacctctat gctgacgcgc atcatgacga tggctgttga
    89401 agatcaccaa ccgccgctgg tacgcggtcg tcgtgtgaag ctgaaatatg cccacgctgg
    89461 tggttataac ccgccgattg tggtgattca cggtaatcag gtgaaagacc tgcctgattc
    89521 ctacaagcgc tacttgatga actacttccg caaatcgctg gacgtaatgg gatcgccgat
    89581 tcgtattcag ttcaaagaag gggaaaaccc gtatgcgaac aagcgtaaca ccctgacgcc
    89641 aacccagatg cgtaaacgta agcgtctgat gaagcacatc aagaaaaata aataattatc
    89701 cgtaagtatt ttatctgcca ttccctctat gaatgtagag ggatggctac cggaggaatt
    89761 catatggaac tgcattgtcc acaatgtcag catgtgcttg atcaggataa cgaccatgcg
    89821 cgttgcccat cctgcgggga aattattgaa atgaaagctc tttgtccaga ctgccatcaa
    89881 ccgttgcagg tgttaaaagc ctgcggtgca gtagattact tctgtcagca cgggcatggt
    89941 ttgatatcga aaaaacgcgt tgaattcgtg ctggcttaat gcaccttttt acgcgatttt
    90001 tttaccggct ggatgttttt tacttcactt tctatccagc cgtcttccag acgtgtggtt
    90061 agcatttcac ccgctttaac ttgcttcact tttttcagta cattgccgtc agtagcagta
    90121 gtaacgctgt atccacgcgc cagcgttgac agtgggctta cggcttcgag gtgcgttact
    90181 gcattaccga aacgttcacg cgtggcgcta agctgtacgc gcagggtttc tgctaaacga
    90241 tattccagtt gctgaatgcg cgtttgcgcg cgatgaatct tcggctgtgg attttgctga
    90301 ttcagccgct gtgttaaccg ctgctgctgt tgcccggtac gcttaagttg attttccagc
    90361 gcaaagctca tccgcttttg caggcgctca agcatggttt gctggcgtgc cagccggagc
    90421 tgcggatgct gttgctgtaa tcggtgatgg atctgcgtaa agcgacgcgt gcggttggcg
    90481 agataataat ccatcgccat ctccagccgt tgacgggtcg attgcacctg gcgcagtaac
    90541 tcttgctgat tacggctcac tacttcagcg gcggcagacg gcgttggcgc acgcagatcg
    90601 gcaacaaaat cggcaatggt cacatccgtc tcatgcccga cggcgctgac gaccggaatg
    90661 cggctggcaa aaatcgcccg cgctacgcgt tcgtcgttaa aactccataa atcttccagc
    90721 gaaccgccgc cgcgcccaac gatcaacacg tcgcactcat tgcgctgatt cgccagttca
    90781 atggcgcgaa cagtttgccc cggcgcgtca tcgccctgaa cggcggtggg gtagatgatc
    90841 accggtagag aaggatcgcg acgttttaac acatgcaaaa tatcatgtag cgcagcaccg
    90901 gtttttgagg tgatcacacc aacgcaatgc gcaggggagg gaagtggttt tttgtattgc
    90961 agatcgaaca atccttcagc ctgcaacttc gctttgagct gttcgtactt ctgttgcagc
    91021 agcccttcac cggccggctg catactctca acgattatct ggtagtcgcc gcgcggctcg
    91081 tagagcgtaa tattggcgcg aactaaaact tgttgcccat gctgtggacg gaaggtcacc
    91141 cgacggttgc tgttgcggaa catcgcgcag cgtacctggg cggtgtcgtc tttgagtgta
    91201 aagtaccagt gaccggaagc cggttgtgtg aaattagaga tttcgccgct gatccaaacc
    91261 tgtcccatct catgctcaag cagcagacga accgtttgat tcaggcgact aacggtaaaa
    91321 attgcagggg attgagaagg taacatgtga gcgagatcaa attctaaatc agcaggttat
    91381 tcagtcgata gtaacccgcc cttcggggat agcaagcatt ttttgcaaaa aggggtagat
    91441 gcaatcggtt acgctctgta taatgccgcg gcaatattta ttaaccactc tggtcgagat
    91501 attgcccatg ctacgtatcg ctaaagaagc tctgacgttt gacgacgttc tcctcgttcc
    91561 tgctcattct accgttctgc cgaatactgc tgacctcagc acccagctga cgaaaactat
    91621 tcgtctgaat atccctatgc tttccgcagc aatggatacc gtaacggaag cgcgcctggc
    91681 tattgctctg gctcaggaag gcggtatcgg ctttatccac aaaaacatgt ccattgaacg
    91741 ccaggcagaa gaagttcgtc gtgtgaaaaa acacgaatct ggtgtggtga ctgatccgca
    91801 gactgttctg ccaaccacga cgctgcgcga agtgaaagaa ctgaccgagc gtaacggttt
    91861 tgcgggctat ccggtcgtta ccgaagaaaa cgaactggtg ggtattatca ccggtcgtga
    91921 cgtgcgtttt gttaccgacc tgaaccagcc ggttagcgtt tacatgacgc cgaaagagcg
    91981 tctggtcacc gtgcgtgaag gtgaagcccg tgaagtggtg ctggcaaaaa tgcacgaaaa
    92041 acgcgttgaa aaagcgctgg tggttgatga cgaattccac ctgatcggca tgatcaccgt
    92101 gaaagacttc cagaaagcgg aacgtaaacc gaacgcctgt aaagacgagc aaggccgtct
    92161 gcgtgttggt gctgcagttg gcgcaggtgc gggtaacgaa gagcgtgttg acgcgctggt
    92221 tgccgctggc gttgacgttc tgctgatcga ctcctcccac ggtcactcag aaggcgtttt
    92281 gcagcgtatc cgtgaaaccc gtgctaaata tccggatctg caaatcatcg gcggcaacgt
    92341 ggcaacagct gcaggtgcac gcgctttggc agaagctggt tgcagcgcgg ttaaagtcgg
    92401 tatcggccct ggttctatct gtacgactcg tatcgttact ggcgtaggtg ttccgcagat
    92461 cactgccgtt gctgacgcag ttgaagccct ggaaggcacc ggtattccgg ttatcgcaga
    92521 tggtggtatt cgcttctccg gcgacatcgc caaagctatc gcagctggcg caagcgcggt
    92581 gatggtaggt tccatgctgg cgggtactga agaatctccg ggtgaaatcg aactctacca
    92641 gggccgttct tacaaatctt accgtggtat gggttccctg ggcgcgatgt ccaaaggttc
    92701 ctctgaccgt tatttccaga gcgataacgc tgccgacaaa ctggtgccgg aaggtatcga
    92761 aggtcgcgta gcttataaag gtcgcctgaa agagatcatt caccagcaga tgggcggcct
    92821 gcgctcctgt atgggtctga ccggctgtgg tactatcgac gaactgcgta ctaaagcgga
    92881 gtttgtacgt atcagcggtg cgggcattca ggaaagccac gttcacgacg tgaccattac
    92941 taaagagtcc ccgaactacc gtctgggctc ctgattctct tcgcccgact tcatgtcggg
    93001 cgatttatat tatctgtttc acttgcctcg gaataagcgt caatgacgga aaacattcat
    93061 aagcatcgca tcctcattct ggacttcggt tctcagtaca ctcaactggt tgcgcgccgc
    93121 gtgcgtgagc tgggtgttta ctgcgaactg tgggcgtggg atgtgacaga agcacaaatt
    93181 cgtgacttca atccaagcgg cattattctt tccggcggcc cggaaagcac caccgaagaa
    93241 aacagcccgc gtgcgccgca gtatgtcttt gaagcaggcg taccggtatt cggcgtttgc
    93301 tatggcatgc agaccatggc aatgcagttg ggcggtcacg ttgaagcctc taacgaacgt
    93361 gaatttggct acgcgcaggt tgaagtcgta aacgacagcg cactggttcg cggtatcgaa
    93421 gatgcgctga ccgcagacgg taaaccgctg ctcgatgtct ggatgagcca cggcgataaa
    93481 gttaccgcta tcccgtccga cttcgtcacc gtagccagca ccgaaagctg cccgtttgcc
    93541 attatggcta acgaagaaaa acgcttctat ggcgtacagt tccacccgga agtgactcac
    93601 acccgccagg gtatgcgcat gctggagcgt tttgtgcgtg atatctgcca gtgtgaagcc
    93661 ctgtggacgc cagcgaaaat tatcgacgat gctgtagctc gcatccgcga gcaggtaggc
    93721 gacgataaag tcatcctcgg cctctctggt ggtgtggatt cctccgtaac cgcaatgctg
    93781 ctgcaccgcg ctatcggtaa aaacctgact tgcgtattcg tcgacaacgg cctgctgcgt
    93841 ctcaacgaag cagagcaggt tctggatatg tttggcgatc actttggtct taacattgtt
    93901 cacgtaccgg cagaagatcg cttcctgtca gcgctggctg gcgaaaacga tccggaagca
    93961 aaacgtaaaa tcatcgggcg cgttttcgtt gaagtattcg atgaagaagc gctgaaactg
    94021 gaagacgtga agtggctggc gcagggcacc atctaccctg acgttatcga atctgcggct
    94081 tctgcaaccg gtaaagcaca cgtcatcaaa tctcaccaca acgtgggcgg cctgccgaaa
    94141 gagatgaaga tgggcctggt tgaaccgctg aaagagctgt tcaaagacga agtgcgtaag
    94201 attggtctgg agctgggcct gccgtacgac atgctgtacc gtcacccgtt cccgggacca
    94261 ggccttggcg ttcgtgtgct gggtgaagtg aagaaagagt actgtgacct gctgcgccgt
    94321 gctgacgcca tcttcattga agaactgcgt aaagcggacc tgtacgacaa agtcagccag
    94381 gcgttcactg tgttcctgcc ggtacgttcc gttggcgtaa tgggcgatgg tcgtaagtat
    94441 gattgggttg tctctctgcg tgctgtcgaa accatcgact ttatgaccgc acactgggcg
    94501 catctgccgt acgatttcct cggtcgcgtt tccaaccgca ttatcaatga agtgaacggt
    94561 atttcccgcg tggtgtatga catcagcggc aagccgccag ctaccattga gtgggaatga
    94621 ttttaatccc tgcactatga atgaacaaaa ccctctgtta ctacagaggg ttttttatct
    94681 tcaagaatca taggcttgaa gttactaaca tcgattaatt aaaccagctg tccgatttgt
    94741 tctcttctgc tttgcccacg cgtttcatca gatcgcgacc gccttcagtc atatttctgt
    94801 tggcgtcagc ttcagattgc accacatcgg tttgcgcagc tttgtgcttc agttcctgat
    94861 cgataaattc gttttctcgc ttaacgcggg cttcttcttt tgccagcgcc agtttttgtt
    94921 tctgaatctc taagctgcgt agctcatctt cataactttg atcgcgtttt ttgtccgcag
    94981 aggcttcggc gtccagttta tcctgacgag ctttcttatt tgccgctgcc gttgccgctc
    95041 ttttgttagc ggcggcctgg gcatttgcgc gacgttgctt ctcttgctgg atttccctgt
    95101 tgcgctccgc gacccattcg tcatgctgcc tttgctcttc atttttacct tgctgttcag
    95161 cttctgctac agcagagagt tgatcctgca atgatgaggc gatagccgga tagcttaagg
    95221 aggccaagat ggcgcaaaga aaaactttct tcatgactcc tcctgattat tagctctttt
    95281 caggacattt agtatttggc tgaatacgcg tttcgttata cgttgtggta ataacaacgg
    95341 ctaaacctgt cgtaaactgg cactctttac ccacctgggt agaggtatac actttggtgc
    95401 cttccttata tgttaaagaa acaccttcca ctaaggtttt atcattcacc atagaaccca
    95461 ctgccgcgcc tacagctccg ccgccaactg cccctgccgt cgttccggaa ttgctgccag
    95521 aaccgacgtt gtggccgata acaccgccag cgactgcgcc aataagcgcg ccgaaggctt
    95581 gtgcgttccg tttattttgg gagttgtcta cggcaacttt tgcgggaaga atggaaataa
    95641 tattaacggt tttagtttct tgtttggtat tcagttgatc ggtttgataa acatcggcgg
    95701 catgatcgtc agcatttgac tggcatcctg ccagagtgaa tgacgctaac attgccacag
    95761 gcagaagaca ttttttaaat ttcatcacta ttccttgtta tacacattac gaaaaataag
    95821 acatttaaga tcatcaaaag aggaaagtaa atgtattgcg tataaaggga tagattaaat
    95881 ttattaaaat tcacgcaggg aataatttat aaaaatatat cattgcaaca tatatattaa
    95941 ttttatattt atttcgcagc caaacaggat aaacatcagt caggacaaag acgtaaaatt
    96001 taagatgaaa gaatatctgc cgctttgata attccaaaga gcaaaagcat ccgacagcga
    96061 agtgccgtta ggttctgtga aagtggagaa ggtaaccgct aacgttgtac cggtgattcg
    96121 acggagagtg tggattgctg agcttctttt ttgctctgat ggtgctgcca ggcaccgacg
    96181 gacgagtaga taaaacggcc aaagaagaag ataaagctga tgagcagtac gatacgggtc
    96241 atgcgactgt taaatcggtg tcgttttcgc atactggttg cctgactcac aaaaggttcc
    96301 ttgaagtatg tcccacgctg tggacggtac ttacattaag gcacaacagg acaaaatggt
    96361 caattcttcg ttatgtaaaa aagcgtcagt ggatacatat tttaatgtta tggaagttaa
    96421 tttaattatt tacaatgatg atgtaataat gatgaattaa catgataaac gtaataattc
    96481 attaatcagg gttattttgt ttgatatata tcaattggaa ctttcatatg acacttagaa
    96541 tcaatgctct ctatagtgat gaataatcat cattcgaagt caggtgggat gcctgtctga
    96601 atacacctcc ttcaggatgt gggggattct gctgagcatc tatgaaactg aatgcaactt
    96661 atataaaaat acgtgataaa tggtgggggc ttccgctgtt cctgccttct ttaatcttgc
    96721 ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaggtt tttctctttt
    96781 atctgccttt ggcactgatg atcagcatga tgatgttttt cagctgggcg gcattgccag
    96841 ggatcacctt agggattttt gtccgcaaat atgcagagct gggtttttac gaaacgctct
    96901 cattaacggc taattttatt atcattatca ttctctgttg gggcggttac agggtcttta
    96961 ctccccggcg taacaacgtt tcacatggtg atacccgttt aatttcccag cgtatattct
    97021 ggcagattgt gtttcctgca acgctgtttc tgatactttt ccagtttgct gcatttgtag
    97081 gattactggc gagcagagaa aatctggtcg gtgtcatgcc ttttaacctc gggactttaa
    97141 tcaattatca ggccttactg gtgggtaatc tgatcggtgt cccgctgtgc tacttcatca
    97201 ttcgggtagt gcgaaatcca ttttatttac gtagctatta ttcgcaatta aaacagcagg
    97261 ttgatgccaa agtcaccaaa aaagagttcg cgctctggct actggcatta ggtgctttat
    97321 tgttgctgtt atgcatgccg ttaaatgaaa aaagcacaat ttttagcacc aattatacct
    97381 tgtcattatt gctgcccctg atgatgtggg gagcgatgcg ctatggttat aagctgattt
    97441 cgctgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat tacattccca
    97501 tttatcctgg ctataccacg cagctgacca taacctcctc cagttatctg gtattctctt
    97561 ttattgtcaa ttatatggct gtactggcaa ctcgtcagcg agcggtagtc agacgcattc
    97621 agcggcttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc ctgaatcgcg
    97681 cgttgcgtga tgccccctgg tctgcgcttt gttatttacg catccctggc atggaaatgc
    97741 tggttaagaa ctatggcatc atgctgcgga ttcaatacaa gcaaaaactt tctcactggc
    97801 tgtcaccctt gctggaaccg ggtgaagatg tttatcagct ttcgggtaac gatctcgcgc
    97861 tgcggctgaa tacagaatcg caccaggagc gcattaccgc actggatagc catctcaagc
    97921 aatttcgttt cttttgggat ggcatgccga tgcaaccgca gattggcgtc agttactgct
    97981 atgtgcgctc gccagtgaat catatctacc tgctgctggg agagctaaat acggtcgccg
    98041 aactttccat cgtgaccaac gccccggaaa atatgcagcg tcgcggggca atgtatttgc
    98101 aacgcgaatt gaaagataaa gtcgcgatga tgaatcgact acagcaggcg ctggaacaca
    98161 accatttttt cctgatggcc cagccgatta ccggtatgcg tggtgatgtt taccatgaaa
    98221 ttcttctgcg catgaaaggt gagaatgatg aactgatcgg ccccgatagc ttcttgccgg
    98281 tcgcgcacga atttggttta tcgtcgagta tcgacatgtg ggtcattgag catacgctgc
    98341 aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg ttttgctatt aatctgtctc
    98401 caacctcggt atgtcaggct cgttttcctg ttgaagtcag tcagttgctg gataaatatc
    98461 agattgaagc gtggcaactt atttttgaag tcaccgaaag taatgctctg accaatgtta
    98521 agcaggcgca aatcaccttg cagcatcttc aggaattagg ctgccagatt gcgattgatg
    98581 atttcggcac cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc gatctgctta
    98641 aaattgacgg cagttttatc cgcaatattg tgtcaaatag tctggattat cagatagtgg
    98701 catcgatttg ccacctggcg cgaatgaaga aaatgcgggt agtggcagag tacgttgaaa
    98761 acgaagagat ccgcgaggcg gtgctctctt tggggatcga ttatatgcag ggttatctta
    98821 ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat cgaacccatt cgcgaaagtg
    98881 cctgaataat gcgggccgac atttctcgtc ggcccgcaaa gcattaagcg gcgatttcag
    98941 gtgtactttc ttcttcaatt ttcaaccgcc agccagccac gccttcccag tattcttgct
    99001 ccttttccag atcaagcagt accagcgcat tctgactaaa ccagtcatgc gggaaacgca
    99061 gtgtccagtg actgtcatca gtaatcagtg tcaatgttgg cggtgtggtg gttgcctgac
    99121 gttgattgtt tagtaatacg ccaaggcgca atagctgtat cagtggcagg aactgtttct
    99181 tcttaaacaa ggtaaagcgc ggcagatcgt cgagcttaat cgctttacgg tgatagcgca
    99241 ccagtgtcgc catcatcagc tgctgttcct gattaaaacc cggcaagtca ctgttttgca
    99301 gaatataagc ggagtggcga tgcaaaccgc tgtggttgat attcaacccg acctcatgca
    99361 gcatggcggc ccatcgcagt agcgcctcca gttgcggatg cgccagcttc ggttgctgtt
    99421 cccgccactg ttcgtacatt tgcatagtgg tatccagcac tcgtcgggcc tgttcgctgt
    99481 cgatgtgata ctggttggcg aggctgctgg cggtgcgact acgcacatcc tgatgacgga
    99541 aacgtccttc catttcatac agtacgcctt cgcgtaacgc cccgtcagaa aggcgcagtt
    99601 cacggatggc taaagcatca aacacaccac ataaaatcgc cagtccagga acgaagactg
    99661 ttttccgctc ttcggaaaga cccggtaaac tcagcgatgc gaaattacgg tgacgtaaaa
    99721 cttcttttac cagtttttcc agacgttccg gggtaattat cccgtctttc tcgcccattt
    99781 ccattaatac ttcatgggcg gcttttatgg taccggaagc gcccattgcc acgttccagc
    99841 cctgaatacg gaattgccag gttaaagttt ccagtttttg tgctgccgcc atgcgagcgc
    99901 gctgaaaatt ctctttattg atgaccccgc cagggaaata aagctgggca aagctgacac
    99961 aacccatccg gcggctttca acgagaatag gttcgaaatt ttcaccaatc accagttccg
   100021 tagatccgcc gccaatatca ataaccagtt tgcgaccttt ttccggttgg gtatgttcca
   100081 cgcccataaa aatcagacgg gcttcttcat taccggaaat aatttcaatc gggtagggaa
   100141 tgaccttttc cgcgcgtttc agaaagtcag tggcgttcag cgcctgacgc agtgtatggg
   100201 tgccaactat acagacgctg gcaggagaaa acccttgtag ccgttcggca aacagcgaca
   100261 gacagttcaa accgcgcgtc attgcctctt cactcaacat attatctggc cccaggccgt
   100321 ccgccagatg cacccgctgt ttcaggcggc caataatctg catggcacca tctaccacac
   100381 gggctatgac catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac tcctgcggac
   100441 gaggggattt atcgtgtatt ggcatagggt tattcaggtt gttcgagtga tttgatgtag
   100501 tcataaatcg ccaactgcgc ccgtactttg cggcgattgc cgcggggaac gtagcgatta
   100561 ctgagttctt tatcgatata acgtgctttg accgtatcgc tgaacaatat gtcgatgatg
   100621 tccagcaccc gctgcttcag gcgcggatcg agcagcggtg tcgccacttc aatacgataa
   100681 tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc gccgccattt
   100741 tcaaaaatat aaacccggtc atgttcaagg taacggtcaa caatactgat ggcacgaatg
   100801 ttgtcgctaa tgccttccag attggggatc agcgaacaca ttccgcgaac cagcagatta
   100861 accggtacgc cggagctgga ggccgcatac agacgatcaa ccaggccttt atcgacaagg
   100921 ttatttagct tcagggtgat accactgggc agcccttgct gcacgttggc gatttcgcgg
   100981 tctaccattt catataacag acggcgagag ttttgcggtg acaccattaa ataatcaaat
   101041 gtcactgggc ggtatgggtt ttcaataaag ttaaataccc gccgtacttc gttggtgata
   101101 cgcgcatctg cggtcagcaa cgaatagtca gtataaagac gcgcggtttt ttcgttaaag
   101161 ttcccggtcc cgatgtgtgc gtaacggacc acttcaccgt tttctttacg tgaaatcagg
   101221 aacagtttgg cgtgaatttt cagccccggc gcagagaaga taacgtgcac gcctgcttcg
   101281 gtcaggcgct tcgcccagtg aatgttggct tcttcgtcga aacgcgcctg taactcaacc
   101341 accacagtga ctttcttacc gttatgtgcg gcgtggatca tcgagtcgat gatgcgtgaa
   101401 tctttcgcca cgcggtaaat gttaatttta atcgccagta cgctcgggtc gaacgaagcc
   101461 tgacgcagca gttccagcac atgctcaaag gtgtgataag gataatagag caacacatcg
   101521 cgttcgcgaa tggcatcaaa accattgcgg aactgggctt tatcaaacca aatgtggcgt
   101581 aaacgcggca gtggtttgtt caccagattg gctttgccga cattggggaa attaataaag
   101641 tctttaaaat tatgataacg accgccgggg acgatggagt cgtagcggga aatagtcagt
   101701 ttttcgcgta acacttcaac cagcgcattg ggcatatcgc gctgataaac aaaacgcacc
   101761 ggctcagcag ttaaacgctg cttgagactg gaagacatca actccatcag gctggcttcc
   101821 atctcatgca ctaaatcgta ttcggcatcg cgtgtcatct tcattgaata ggcattcagc
   101881 gcgtcataat caaagaagcc tttgaaaata tcatcaaggc agtaacgcag aatgttatcc
   101941 agaagaatca tcggcttgcg tcgacgcggc gcttctggcg gtaaattcac aaagcgcggc
   102001 actttatctg atgggatctc cagcagcgcg taacggatgg tatcaccacg gataatttcc
   102061 accgccagat aggtgtaatc atccttcagg aactgcacta agtcagtgtc aggattgatt
   102121 aaaatcggcg taatgtgctg acgcagatac tgcttaaaat aatgacgaag ccagttttgt
   102181 tgattgacgg agagctggcg ttcattaatc aggaagatct ggttgcgcgc catctccagc
   102241 aatagctcgt tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg ggactgaatt
   102301 ttgcccagta aatggcggga atgagagttg gagccttgtt cttcgctaat aatgatgcgt
   102361 cgcttcagtt cagcgaagcg gactttatag aactcatcaa ggttattgga atagatcccc
   102421 aggaaacgca tcctttcaat cagcgggtta gatttgtccg ccgcttcctg aagcacgcgt
   102481 tcattgaacg ataaccaact gagctctttt tcgatgtata gcttttcctg acccattacc
   102541 acttttactc cgttttattc acgggacact gcctggatat tatggcgagc aatattggca
   102601 gatgtccaac tatgccagaa aagggggaaa aaaactgctg gagcttgcga tttacaggtg
   102661 aattgaaaaa cgccagcggc agagctggcg ctttaactac ggggcattac tcgtcggcag
   102721 cgtagccctg cggcggcaga cgttgaccat ccagccacgc ggcgttttcg tgcattttca
   102781 gacgaccatc ggcaaaccag ctaatcacca gtggataaat agcgtgttcc tgggtttgca
   102841 cgcgggcggt gatgtcatct tccgtatcac cagcaaatac cgggactttc gcctgtaaaa
   102901 taaccgggcc accgtccagt tcatcggtga cgaaatgcac cgatgtaccg tgctcttcat
   102961 caccattttc cagcgcttga cgatgggtgt gtaatccggg atatttcggc agcagagaag
   103021 ggtgaatgtt cagcaaacgc ccggcatagt gggagacaaa cgccgggctg agaatgcgca
   103081 taaaaccagc cagcacgacc acatcgggtg cgtacatgtc gatttcatga atcaactccc
   103141 ggtcataggc ttcacgactg tcaaacgcgc tggcgatgag cgtatgcgtt gcaatacccg
   103201 cctggcgggc gcgttcaagg ccgaacgcgt cggccttatt gctgaaaact gcccgtacgg
   103261 tgcctttaat tttgttggtt ttacaggcgt caataattgc ctgtaaatta cttccgttac
   103321 cggaaataag caccacaata ttcattattc gataaccacg cgttgttcgg aatcagaggc
   103381 tttgatgata ccgattttcc acgcgttttc accgttggca ttgagcaggg cgagggcttt
   103441 gtccacttcc ggagcaggca gggcaataat catcccgacg ccgcagttga aggtgcgata
   103501 catttcatgg cgctcaacgt tacctgccgt ttgcagccag ttgaacactt ccggccactg
   103561 ccaggaagat tcatcaatca ctgcctgagt attatctggc aatacgcgtg gaatgttttc
   103621 ccagaagccg ccgccggtca ggtgcgcaat ggcatgcaca tcgacctttt caatcaactc
   103681 cagcactgac ttcacgtaaa tgcgggtcgg tgccagcaga taatcggcta atggcttacc
   103741 atcaagttgg gtggtttgcg gatcacaacc gctgacttca agaattttgc gcaccagcga
   103801 atagccgttc gagtgcggac cgctggaacc gagtgcaatc agcacatcgc cgtcgctgac
   103861 tttagagccg tcgatgattt ctgatttttc taccacgcca acgcagaaac ccgcgacatc
   103921 gtaatcctca ccgtgataca tccccggcat ttctgccgtt tcgccaccca ccagtgaaca
   103981 gcccgattgc agacatcctt ccgcgatacc gctgatcacc gctgaagcgg tatcaacatc
   104041 cagttttccg gttgcgtaat agtcgaggaa aaacagcggc tctgcacctt gcaccaccag
   104101 gtcattaacg cacatggcga ccagatcaat accaatggtg tcgtgacgtt ttaagtccat
   104161 tgccagacgc agcttggtac ctacgccgtc agtgccggaa accagcacgg gttcacgata
   104221 tttttgcggc aatgcacaca gcgcaccgaa gccgcccaga ccgcccatca cttccggacg
   104281 acgcgttttc ttcactacgc ctttgattct tccaaccaga gcattacccg cgtcaatatc
   104341 aacaccggca tctttgtagc taagagaggt tttatcggtc actgcttggg tccccacgcg
   104401 ttacttgcgg tagaaaaata aaattcggcg caattctaac agggaaagca aacgtttgcg
   104461 agactgcttt acacaacctt tttgcacgtc ttttgcccag gcgcgcggcg aaagaagact
   104521 tgtaccaggg taaaggttag ttttcggatg gaataatctt ctttcataac catttgaata
   104581 taaaataact ttatctcaag ccgttatcat tttgactaaa gtcaacgaaa agaatattgc
   104641 cgccttgaag aaaggaggta taatccgtcg attttttttg tggctgcccc tcaaaggaga
   104701 aagagtatga agatcgtgga agtcaaacac ccactcgtca aacacaagct gggactgatg
   104761 cgtgagcaag atatcagcac caagcgcttt cgcgaactcg cttccgaagt gggtagcctg
   104821 ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag taactatcga aggctggaac
   104881 ggcccggtag aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt
   104941 gccggtctgg ggatgatgga cggtgtgctg gaaaatgttc cgagcgcgcg catcagcgtt
   105001 gtcggtatgt accgtaatga agaaacgctg gagccggtac cgtacttcca gaaactggtt
   105061 tctaacatcg atgagcgtat ggcgctgatc gttgacccaa tgctggcaac cggtggttcc
   105121 gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg
   105181 ctggtagctg cgccagaagg tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg
   105241 tataccgcat cgattgatca gggactgaac gagcacggat acattattcc gggcctcggc
   105301 gatgccggtg acaaaatctt tggtacgaaa taaagaataa aaataattaa agccgacttt
   105361 aagagtcggc ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat
   105421 gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag
   105481 tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat
   105541 taacccggcg actgtactgt tatttaacgg tattggaacg ctgctgtatc tcttcatctg
   105601 taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac cggtattgtt
   105661 actgttgccg ttagggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt
   105721 ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc
   105781 acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc
   105841 cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat
   105901 ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc
   105961 aattatcccg attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat
   106021 tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccaaccc tctatacgcc
   106081 gcgcttcgag tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga
   106141 acacgtaggg cacctggtag taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc
   106201 aggtctgcac cgttcgatgt ttgctaatgg cttgtcgacc gtgatttccg gcttctttgg
   106261 ctctacgcca aataccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta
   106321 cagtacctgg gttatcggcg gggcggcgat tttcgctatc ctgctttcct gcgtcggtaa
   106381 actggctgcc gctatccaga tgatcccatt gccggtgatg ggcggcgttt cgctgctgct
   106441 ttatggtgtc atcggtgctt ccggtattcg tgttttgatc gaatcgaaag tggattacaa
   106501 caaagcacag aacctgatcc tgacttccgt gattttgatc atcggcgtca gtggcgcgaa
   106561 ggtaaacatc ggcgcggcgg agttaaaagg tatggcgctg gcgaccatcg tcggtatcgg
   106621 cttaagtttg atcttcaaac tgatcagtgt gctgcgccct gaagaagtgg tgctggacgc
   106681 agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc
   106741 aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat
   106801 ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg
   106861 caagtttctg gccgggggat aactcctctt tactggctgc gctgcaaaac gtgctgcgtc
   106921 aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc
   106981 tgcacgcggc ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat gtcccgctgg
   107041 ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct
   107101 gtatcgacaa cattgagtgt attgcaggcg atgagttgtg ggagatggcg attttcgatc
   107161 tctacaatcg aattctggaa tcgggcaaaa cacgattgtt gatcaccggc gatcgtccac
   107221 cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg actcgactgg gggcagatct
   107281 acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt
   107341 tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag
   107401 aaatgcgcac gctatttatg acgttggatc agttggatcg tgcgtcgatt accgcgcaac
   107461 gtaagctgac cattccgttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt
   107521 cggatgcggc gcgaacgcct tatccgaact acgcgaagtt aatccatagt attcgtaggc
   107581 cggataagac gtggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat
   107641 cttaacccac gatttccagt acctgctccg gtggacgacc aatccgcgct ttgccattcg
   107701 cgaccacaat cgggcgctcc attagcttcg gattatccac cattgcctga atcagcgcct
   107761 cttcgctaag cgaactgtca gccaggttca gttctttata aagatcctct ttctggcgca
   107821 tcaattcacg ggcgctattc atccccagca ttttcagcaa atcgcgcagc gttgccgcat
   107881 cggcgggtgt ctcaaggtag agtaccactt ctggctccac gccgttttct ttcagcaggt
   107941 tcagcgtttc ccggctcttt gaacagcgtg ggttatggta gatttttacc tgtttggtca
   108001 ttgttcttcc tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg
   108061 gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg
   108121 cgaactggcg ctactcaaca gcgaaatggc ctgatcgagt cgtccggcga gcgcataacc
   108181 ttctgctcgc gcagccagct cctgatcgcg gttatttagc gcggcttccg cctgtgccag
   108241 taaatcccag ccgttgctgt catctttatt attaaaggtg tagcgattca gaatattggc
   108301 cgcttcttgt ggttgaccgc cttgtagata agcgttcgcc aggttgagct gcaacaccgg
   108361 attggtgcgc aaatcacggg catttttcag gcgattgatt gcctcattgg ctttgttttg
   108421 cccaagatcg atatcggtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag
   108481 taacggttgc agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtaaagc
   108541 acgaccatat tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa
   108601 atcactggtg agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag
   108661 atagaaatct tccgacgact gcaccaccat cggtcgcatc tgattagcac ggttgcgggc
   108721 atctgccaga cgactttccg gcaacgggtg agtcaataaa atttccggcg ggcgcgagga
   108781 gtaacgcgcc tgatcgagta atttttcgag gaaggttggc atcgcctgcg gatcgaatcc
   108841 cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa
   108901 actgatcatt ccctgacgtg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg
   108961 cggactggcc atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct
   109021 gcgttgttga tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg
   109081 cgccataact gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag
   109141 caccacgttg ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaaaaa
   109201 atgaaacggt gtcttaaccg aattggcatg cgaaaccaga cgcatcccca gcgaattaat
   109261 atattgcgtt aacagcgggt cattaattaa cggcgcgctg ccgcgtagct ggcggacata
   109321 atagtcgccc atctgcattt cctgaccaat ggaaagcgtg cttcctgcgg aggttcccat
   109381 atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc
   109441 agcaatgagg gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta
   109501 tttctgaaga tagtcatttg accgatactg ttaaatatcg ttcccaccgc gccgcctttg
   109561 agtgtaacgg cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa
   109621 aaagcgaagt cttttttgtg acatttcgat acaattgcag atcgcaatcc cgctcttgag
   109681 gttcagggaa ggtttttatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg
   109741 acccggaagc gattgccttg ctggttattt tagttgccgg atttggcatt atctttttct
   109801 ttagtggcct gcttgctccg ttgctggtgg ctattgtgct ggcctatttg ctggaatggc
   109861 caaccgtgcg cctgcaatct attggctgct cccgccgctg ggcgacgtcg attgtattgg
   109921 tggtttttgt cggtatattg ctactgatgg cgttcgtggt actgcctatc gcctggcaac
   109981 agggcatcta cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg
   110041 ccacgttgcc gcgccgctat ccggcgttaa tggacgcggg cattattgat gcaatggccg
   110101 aaaatatgcg cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct
   110161 cgctggtcgg tttgctgacc attgccgtct atctggtgct ggtgccattg atggtcttct
   110221 tcctgctgaa agacaaagag cagatgctga acgccgttcg tcgggtgctg ccgcgtaacc
   110281 gtgggctggc aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg
   110341 gcaaagtgct ggagatgatc gtggtaggga tcgccacctg gctggggttc ttgctcttcg
   110401 ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt ctcggttctt attccgtaca
   110461 ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggcgcag
   110521 gcacggaatt ctggagctgc ttcgcggtgt atctgattat tcaggcgctg gacggcaacc
   110581 tgctagtacc ggtgttgttc tccgaagcgg ttaacctgca tccgctggtg attattttat
   110641 cggtggtgat cttcggtggt ttgtggggat tctggggcgt attcttcgcc attccattgg
   110701 cgacgctgat caaagccgtg atccacgcct ggcccgatgg gcaaatcgcg caagaataat
   110761 cttcagcccg acgaactgtt tcgccgggct gatttttcag gattcctgac gtaaataaat
   110821 agcccgataa cacatactca ccagcactgc accgccgata atattaccca gcatcaccgg
   110881 gagcagattg gcggtaataa aatggctgac cgtcagtgcc ggaaaattgt ctgcgctact
   110941 gtgcgccagt tgccagaagg aggtaggggc gaaatggcga atggcaatgg cgaacggaat
   111001 cacaaacaaa ttggcgatac agtgctcaaa gccactggcg acaaacaggg tgatgggcaa
   111061 aatcatggcg acgattttgt cgcataacga acgcccgcaa taactcatcc acagcgccag
   111121 gcaaaccatc agattgcaca taatgccgag gctgacagat tcagtaaatg tatgatgcat
   111181 tttgccctcg gcgcagtgta aaaccgcgac tccccacatc gcgttttcac tcatcaccag
   111241 cccggaaaac cagattaaca aactgaaaca ggcaatacct gccagattac cgcaggccac
   111301 cagaagtgcg ttaatcagcc aagttcgcca actaataacg cccctacttt ttgccattac
   111361 cgtcattacc gacgaggtga acaggttggt gccgcaaacc gccagcaaaa taaagccaag
   111421 tgtaaagcaa aggccgccca ccagatgggt taatgcctgt gaagacgggg catcggcgat
   111481 aactgaaagg taaaaagtaa atccgatcgc cataaacacg ccagcggaca tcgccattaa
   111541 aaagaccgac actttactgc gggcaatttt atgggcggca atccgttcag cgattgccgc
   111601 ttttctggcg ctcaactgca agtcgaaaga gagtttgttt ctcatgcata ctcctgcaaa
   111661 agcagattac aacacctcgc gaaccgagat tcccagacgc tgcattcgtg acagcagcgt
   111721 ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt
   111781 ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg
   111841 gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa
   111901 acggctttgt cggacattaa gatgtaaatt cagactgtta ccgcgagtca gcagtaccgc
   111961 tcgctcaatc acgttttcca gctcgcgcac gttgcccggc cagtcccacg acatcaactg
   112021 gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc atatggcgcg ccattttttg
   112081 cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg gcggcaattc
   112141 cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat
   112201 ttgccacaaa tcacggttgg tggcggcaat gactctgaca tttaccggga tcgttctact
   112261 cccgccgaga cgctcaatct cccgttcctg caatacgcgc agcagtttag gctgaagttc
   112321 taacggcaga tcgccaattt catcgagaaa caacgtgccg ccatcggcaa tttcaaaacg
   112381 accacgatgg gtattaatcg caccagtaaa cgcccctttg tcatgaccga ataactcact
   112441 ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg
   112501 cgggctaagt tgatggatcg ctctggcgat cacctctttg ccggttccgg tttcaccgca
   112561 aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagta ggtcttccat
   112621 tgcctggctt tgatagataa tgtcaccgat gcccagattc gaaaggagct gctcgctaag
   112681 ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctacgcc aggcatcggc
   112741 attgccaacg gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc
   112801 actaaagaga gtggaagagg tatgcgccag caacaatgca cccggtgtat ggttgccaaa
   112861 ggtaagcggt ataaggagcg cagattcgca gccgttagat gcaagtaaga gtaataacgg
   112921 gtcgcgttgc cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt gtcgggtttg
   112981 tagcgtttgt gtcagcaata cactgtcgcc aggcatattg cgtggcagac acgcacaatg
   113041 tgaggcagaa agatcgctgc accacaggct aaacttctcg ttctttcgat gatcgcccag
   113101 taccatactg actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat
   113161 cagatcgtcg agatcaagat gtgacagcac tgcattagta atatcaacca gtacccgatg
   113221 atgatcgcga tctttctcca gcaaacgcag cgccacctgc tgttcttcat cattgagcca
   113281 tgattgcaga accggggtga ttatccgcgc cagccagtta aacagtgtgc aatcctgggg
   113341 agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc gccattccac tactgccgag
   113401 aaggacggcg tgacgcgaat aatcgaccat ctcgccctga tactctgttg ttgccgtcac
   113461 atcgctgcca tcatcatggt agcggtaata gaagttgctt ttgagaacat gcagttcaat
   113521 aagttgtacc gggctgaacg gcggcggcat cgggatgaag gcgcgtaata aagacgcctt
   113581 accgtgtttc tgtgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc
   113641 ttgtggcggg gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga
   113701 aatccttctc acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca
   113761 tccaacagat gaatgaattg aatgctacgc gcctgccaca cagacgtcgc gtcatccgcc
   113821 agtggcgcga atgcccgggt caacagggcg gtgtggctgg cgtcaataac aatctcacca
   113881 aaagtcatca cccccagcat cttacgtcgg gcttgctcct cttcgacctg ggcaagccaa
   113941 tcttcgtatt ccgtcagtgg gcaggggaag gcgacattca gacaatcaat cacgccaacg
   114001 tgatgaccga tagccagcga gtaatacatt acctgagagc tgtgttccgg catctcgtcg
   114061 ctactggcga caaacttttt tcgcagcgtc cagaaaacaa tttccccgca ctcttcagtc
   114121 atgtaacccc cggagtacgc gaaagagttg ctgaacgata ctattgagac gtggatcgtc
   114181 ggcatcgcgc aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag
   114241 gagccgatcg caaatttctc gcccctgacg atagcccgcc agccgccgcg cttctcgctc
   114301 aatggcgata cgctgtgatg gcgggatctg cggccacaac ggttgcatag tcaccccggt
   114361 gggatcgcga taatccaccg cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa
   114421 accgtgaatg gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac
   114481 aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg aaaatccgcc gccggcaccg
   114541 cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca
   114601 ggcatacgca tcgcccgggt gactgcgcct gtaaataaca aaatatcggc gtgacgcggt
   114661 gatgaaataa ccttaatgcc aaaacgttct gcgtcgaata ctggtgtgat ggcggcaaaa
   114721 atttcgattt cacaggcgtt gcagccgccg caatcgacgc gataaacgta agccgagcga
   114781 cggatatcct gtagcaaatg ctgcttcatc ttttgcgttt gctcgtccag cgtgatgggc
   114841 tggctgacat gttgtgtaag cactggactc atagctgctc cttagccacc agcggtacat
   114901 ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg
   114961 cccataacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca
   115021 gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga
   115081 acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa
   115141 gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata caacgcctca
   115201 gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc agcgcatttg
   115261 ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc aggtccggtt
   115321 tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc gtcgcggttc
   115381 cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc agcggcgaac
   115441 ggttacgatc aataccgtag cgttcgatct ctttatacgg cacggttttt gactggcgct
   115501 tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca aggctaccga
   115561 taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac accggccagt
   115621 tggcgtaggt ggcggcacgg cagcgccagc ggaacaattt ttggttatca ccgagcatgc
   115681 tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat gcgtgaggtt
   115741 tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg gcaaattcca
   115801 gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag aaaacatcgc
   115861 cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag gggtgatcaa
   115921 agcgcaaatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg cgttgttcca
   115981 tattcggcgt agcaagcagc atctctacca gctccgacac gtcggcgcgc atctcgcgca
   116041 ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca ccaccaatca
   116101 gattcagacc gtaggtttta cgcgacccga tcagcaattc cgccatcgtc atcgactttt
   116161 cccgcacgcg gaaaaattgc ataaagccgg tatcaaaacc aacgaaatgg caggagaggc
   116221 caaggttaag caaatgactg tgtagccgtt cgacttccag cagaatcgag cgaatagtat
   116281 gtgctcgttg cggcacctca atccccagtg cattttcaac cgagttggta taggccacac
   116341 tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact tcgttgtagc
   116401 ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacagg cggtaatcag
   116461 catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccagttca tcggaggtga
   116521 tatgcagcgg gccgacaggg atcacctgcg catcgctgtt gccctcattg ataaacggat
   116581 acgtttcgga atcagtcgtc ggttcagggc gcagtcgata atccatcgca tctttgcgca
   116641 gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgatga tccggcaggc
   116701 caaccggaat cagcccgtac atatcgcgaa tttctcgctc gccccagacc gcggcaggga
   116761 cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagtgcc ttcaccacga
   116821 tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa tgaccgttaa
   116881 gtgtccgctc gtcgttgcca aacaggactg gaagccagcc atcatgttga taataaagat
   116941 aatgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt tcaggcgttt
   117001 gtcgctcttc atccagcacc gcgccgggga actgcgtttt cagggcggcg agaatcgctt
   117061 cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct
   117121 gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg
   117181 actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg cgttcccatc
   117241 gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc
   117301 cggttaacgg cctgcggcgg tttcgccatt aacacccgcg ctgccatccg taccaggccc
   117361 gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg attacgtgcc
   117421 agtccggcgg taatggtcat aaattcgcta agaaaaatgt tgaagggcgg catccctgcc
   117481 agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt gagcatccca
   117541 cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga accgcagaac
   117601 agcagcgttt ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa aattcccagt
   117661 ggcccgccaa tgcctagcgc caccgcgacc agtcccatgt tctccacgct ggagtacgcc
   117721 agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac
   117781 atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg gcagataatg
   117841 atgtaatagc gaattagcac caacagcgcg cagttcagca atacggcgga gagcagggcg
   117901 ctgaccggac tcggtgcttc actgtgagca tccggcagcc aggcgtgcat gggaaatagc
   117961 ccggttttgg tgccaaagcc aattagcaca aacacaaagg ccaacagcat taatgttggg
   118021 tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc tgcctgcggc
   118081 ataacgctgg cgacgttggc gtataccagc acggtaccga acagaccaaa agcgacacca
   118141 acagtacaaa taatgatgta cttccatgct gcttccagcg atgaacgctg accgtaaatg
   118201 cctaccagaa acgccgagct taaggtggtg gcttcgatcg ccgcccacat cacaatcagg
   118261 ttattgctgg taacaaccag cagcatggta aacaaaaaca gatggaagaa accgtagtaa
   118321 tcgcacagcg taacggggga aagctcgccg tgtgccactt catgacgcat gtagccaatc
   118381 gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa accgcccaga
   118441 ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg
   118501 accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa tacagtcacc
   118561 gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga aaaaagcagc
   118621 ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc ccttcagcgc
   118681 ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg ccagtaacac
   118741 catcacaatg acggcgaaga tggcgtcggt agcgatacct atttccacca gttccggtgc
   118801 tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc agtaaccaaa
   118861 aatttgccgc aggatattgc gctggctgac aatgcacagc aggccaagca gaaaatgacc
   118921 taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca cgggctgaac
   118981 gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg ccggaccgaa
   119041 taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca tgattaacgg
   119101 taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt gctctgcggc
   119161 gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc aggcgtaaaa
   119221 tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca gcatcatcag
   119281 tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta ctgcaaccag
   119341 gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa caccaggcgc
   119401 attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc aggcacaacg
   119461 cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc
   119521 atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc aaacagcggg
   119581 aatttactga agaagccgtt gaacggcggc acgccggtaa tcgccagcgc tgccacgcag
   119641 aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg caacaagcgc
   119701 gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt agcgaacgcg
   119761 tggttgacga tgtaggcgat gctgccctcc agcgccagcc gcgagccgaa gatggagagc
   119821 gacaaaccga agaacatcca gccaagttga gtgatggtcg accaggccag caaccgcttc
   119881 atatcctgct gtggcaaata catcagaaag ccataaagaa tggtgaccag cgccatgacc
   119941 atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataatggc gcgggcaaaa
   120001 atgtaaacgc ccactttcac cttcgatgcg gcgtggagat aggcactgat cggtgttggc
   120061 gcttccattg catccggtag ccacgcttgc atcggtagct gggccgattt cccccacgcg
   120121 gcaaacagga tgccgccata aaccagataa cgtgcgtcgc cgtgtaactc gctcatcgcg
   120181 ctaagcgcaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata caaccccaac
   120241 gagccgatat gagtgataag taacgctttt agtgctgaac gctgtgcttt atcgctctgg
   120301 taataactga tcaacgccca ggagcagccg cccgtaattt caaaaaacaa caactgaccg
   120361 agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaacag caaaaatgcg
   120421 taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag ataacccgtg
   120481 gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag cgtactcacg
   120541 cgatcaatga ccaggccaaa caatgccaca tcccccacgt tgaccaatgt caacgtgacg
   120601 gcaactttat cggcctgata aaacgcggag atcagtgaca acatgcacag cgtggtcagc
   120661 gcggcgaaca aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt
   120721 gcgccgataa aaggcagcaa taacgtcgtc agagcaagat tttccatatt ccgtcagtgc
   120781 tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga agccaagcca
   120841 ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg cgattgaggc
   120901 cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc
   120961 gagagaaagt tcttgcgcgc gcccaaagga cagaaacagg gccacaaaca gtgatgccat
   121021 cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac cggaatattc
   121081 ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg gaatttttcc
   121141 catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg ttagcggcga
   121201 gttccagccc atcgccagcg tattgctgat catctcgata tgcgtggaac ctgctatcag
   121261 cgccaatacc agcagtgaga gaataagcat tggttcgacc agaatgccga gcgtcaactc
   121321 gcgactggca ccgactcccg caaacggact tccggtatcc agcccggaaa gagcaaagaa
   121381 aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg
   121441 ggaaacggta ataaacagtg gtaaggccat cgccagcacc agcatgctgc tgattaatac
   121501 ccacggcatc aggcggaaca tcagacctga agatgtcggc gcaacttcct ggcgtttaaa
   121561 cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg agtgcatacg
   121621 cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca gaatcacggc
   121681 ctgtgctaac gcaaaaataa ccagatatcc gtcgcaaaga gtttgtctca tggtgatttc
   121741 cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac aggcaataga
   121801 gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg gtggcgctca
   121861 cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc ctggcggtga
   121921 cgtgcgcaaa acctttattt agcctcagcg tagggtcgag ctgttgtcgt agacgaaata
   121981 acgcagagaa gaccacacgc agcggctgca tcacgccatt gcctgacggg gccatcgcat
   122041 tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg
   122101 aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt aacagcaaca
   122161 gtaatgaggg ggtgacctgt gtatgaaacg tgccgggtac aagtgctatt ccgctggcga
   122221 cagtgacggg aggggtattg gtaaacgcat gggcaatatg cattatcttc ggtgccagcc
   122281 aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg gcgacgatca
   122341 tcgtatttgg cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca ccacagaaag
   122401 taataccgta catttttacg aagcacatta ctgccagccc accagtgact gccagcatta
   122461 ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt agtgagaaca
   122521 gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg gctgatatcg
   122581 cgaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc agtgccccca
   122641 ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc gcccgcgccg agaaatagca
   122701 gccctttgaa cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg
   122761 tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgactgcc agcaaaataa
   122821 tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt tccgccagcg
   122881 catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga atgccccacc
   122941 acagcggcaa acccgtttgc gccagcaggt ccatcgctac tttcaggatg ccgaaaatac
   123001 cgattttgac cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg gcagggtgag
   123061 cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg
   123121 ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg aaactggcaa
   123181 aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcataatc agcaccgatc
   123241 cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta tcctgcctgg
   123301 caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag
   123361 cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aagattcata aagaagccca
   123421 tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag agcgaacaaa
   123481 ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc attcgcaggg
   123541 taaaatcggc aaagtcaaaa ggggtggcaa aacgggcgac taaagttaca ccagcatgta
   123601 attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg atgccggaaa
   123661 gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg gcaaacagat
   123721 agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct cctgagtgag
   123781 ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt
   123841 catctgtcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg
   123901 aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg cgacggtctg
   123961 gacacccggt tcccagcgca gcaatgcatg ttggggcggc acgttttctt cgccgtcttt
   124021 gagtgagcct tcagcctgaa aaacatattg cgcaggggca ttcaccggac ggcttcctga
   124081 agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct
   124141 ttcgttgagt tggatcgcat catccctctg ggagatggca ttaaccgggc agacctgcag
   124201 gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg acgtcttcgc
   124261 cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc
   124321 gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc ggttcatgtc
   124381 ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat gccattctga
   124441 ttaactcaat atattcaata agttatgtat tgactggatt ctgaacggta caaaaatgat
   124501 ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga aagcgagaat
   124561 gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc acggaatgga
   124621 gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt gattgctggt
   124681 tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga agctgatgcg
   124741 atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc cgaattgttc
   124801 gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt tttccgcaaa
   124861 acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc ccgctttttt
   124921 caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg gggtcatggc
   124981 tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat taacttgttc
   125041 tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca gtggattcat
   125101 tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat actgccttgt
   125161 gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc gtccgcagat
   125221 gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt
   125281 tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag
   125341 cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga tgctggcatt
   125401 ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag ttcggcacct
   125461 ttcaacggta acgttgattc aatcagagtg atggcattcc atgaaccgga aagcagcatg
   125521 ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt acagccgcaa
   125581 ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc caacgcagtg
   125641 atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag gggtgagttg
   125701 ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc tgtctgcttg
   125761 cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg atggcccatg
   125821 ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg
   125881 gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc gatcgtttct
   125941 gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga tgtggtgatg
   126001 atgacgctgg atctggctga tgggcgcatt ccggtaattg ccgggaccgg cgctaacgct
   126061 actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg
   126121 acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc
   126181 atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg tactggctgc
   126241 gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa
   126301 gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc agatgatttt
   126361 gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattggg cggtcatggg
   126421 gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg caaactggca
   126481 gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc attacacaac
   126541 aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga actgggtctt
   126601 gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg tcgtgagacg
   126661 gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt tgatggctta
   126721 ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc
   126781 tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag cctacctgga
   126841 agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg tgacctccgg
   126901 tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg
   126961 tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca cgggcgatac
   127021 cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg tggttagcgt
   127081 gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga cactgaccac
   127141 cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc gttatcaaat
   127201 ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgctga acctggaaca
   127261 ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg agatgatgaa
   127321 cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg
   127381 tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac caatgctggt
   127441 cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg aaaaagtggg
   127501 catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt ataagccgct
   127561 gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat ccggtgacta
   127621 taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg atccgaaagg
   127681 tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg ctgcgtttag
   127741 caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat acgcaaacgt
   127801 gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt ttgtcggacg
   127861 tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc agacagcaaa
   127921 agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca agctgagttg
   127981 tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa
   128041 ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga tcgcaaaggt
   128101 atggtgaaca acaagttcaa ttacttcatt atgagcaaac tggctgaagc gggtatcccg
   128161 actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg
   128221 ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg tcttggaatc
   128281 gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg
   128341 cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag caaagagaac
   128401 ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat
   128461 gatgctggtc tgattctggt cgacttcaag ctggagtttg gcctgtacaa aggtgaagtg
   128521 gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga aacactggag
   128581 aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc
   128641 gtcgcccgcc gcctgggtgt acagctggac tgatttttct ggttgcttgt ttctttagcc
   128701 ggagggtact ccggctaaat gtctttgata cccccgctac aatacttctc attatctccc
   128761 tttgacagga aagtcatacc aggccaatat gtatatacat aacgtatatc ccaaaaaggg
   128821 ggggctatgg aaactactgt atttttaagt aatcgcagtc aggccgtcag attaccgaaa
   128881 gcggtcgctc tgccagaaga cgttaagaaa gtagaaatta ttgcgattgg tcgtacaaga
   128941 atcatcactc ccgccggtga gtcctgggac agttggtttg atggcgaaaa tgtgagtgcg
   129001 gattttatgg atatccgtga tcaacccgca atgcaggaaa gggaatcatt ttgatgctga
   129061 aattcatgct ggataccaat atctgtattt tcactattaa gaataaacca gtacatgtca
   129121 gagagcggtt taacctgaat tctggccgga tgtgtataag ttctgtaaca ttgatggagc
   129181 ttatctatgg ggcagaaaaa agtcagatgc cagaacgcaa tttggcggtt attgaaggtt
   129241 tcgtatctcg tttggtagtg cttgattatg atagtgctgc tgcgacgcat accgggcaaa
   129301 tcagggccga gcttgcccgg cagggaaggc ctgttggacc ttttgaccag atgatagcgg
   129361 gacatgctcg cagtcgcggt ttgattgtgg ttaccaacaa tacccgagag tttgagcgag
   129421 tggcgggaat cagactagaa gactggagct aacattctct tttacttcta acattcgtaa
   129481 attatataac gtggcatgaa gccttaattc ctccgccatt tcatcccgct ttcctgtggt
   129541 aatcttcctc tgaaccacct acgatcattt ctataatgaa tatattgttg aggtatctat
   129601 gcgttggcaa gggcgacgtg aaagtgacaa tgttgaagac aggcgcaaca gctccggtgg
   129661 tccgtctatg ggcggtcccg gttttcgcct gccaagcggt aaaggcgggt tgattttact
   129721 gttagtcgtg ctggttgcag gctactatgg tgttgattta accgggttaa tgactggaca
   129781 gccggtttcc caacaacaat caacgcggtc cattagccct aatgaagacg aagctgcaaa
   129841 attcacctcg gtgattctgg caaccacgga agacacctgg ggacaacagt tcgagaagat
   129901 gggtaagacc tatcagcaac cgaagctggt catgtaccgt ggaatgacgc gcaccggctg
   129961 cggggctggt cagtccatta tggggccgtt ctattgcccg gcggatggca cagtttatat
   130021 cgatctctcc ttctatgatg acatgaaaga caaacttggc gcggatggcg attttgccca
   130081 ggggtacgtt atcgcccatg aagtcggtca tcatgtgcag aaactgttag gcatcgagcc
   130141 gaaagttcgt caactgcaac aaaacgcgac gcaggcggaa gtgaaccgct tatctgtgcg
   130201 tatggaactc caggccgact gttttgccgg tgtctggggg catagtatgc agcagcaagg
   130261 cgttctggaa accggcgatc tggaagaggc gctgaacgcg gcgcaggcca tcggcgatga
   130321 ccgtttacaa cagcaaagtc aggggcgagt agtaccagat agtttcacac atggcacttc
   130381 tcagcaacgc tacagctggt ttaaacgtgg tttcgacagc ggcgatccgg cacaatgcaa
   130441 tacttttggt aaaagcattt aacttccggg gcagggatgg ctgaactgac tgcgcttcac
   130501 acattaacag cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt gagcggggaa
   130561 gagggttggt gttttgatca tgcgcttaag ttgcgtgatg ccttacctgg cgactggctg
   130621 tggatttcgc cgcagccaga tgctgaaaac cactgttcac cctcggcgct acaaacttta
   130681 cttgggcgcg agttccggca tgcggtattc gacgcccgcc acggctttga tgccgctgcc
   130741 tttgcagcac ttagcggaac gttgaaagcg ggaagctggc tggttttgtt actccctgta
   130801 tgggaagagt gggaaaacca acctgatgcc gactcgctgc gctggagtga ttgccctgac
   130861 cctattgcga cgccgcattt tgtccagcat ctcaaacgcg tacttacggc ggataacgac
   130921 gctatcctct ggcggcaaaa ccagccgttc tcgttggcgc attttactcc ccgtactgac
   130981 tggcaccccg cgaccggcgc accacaacca gaacaacagc aactcttaca gcagctactg
   131041 accatgcctc cgggcgtggc agcggtaacg gctgcgcgtg ggcgcggtaa gtcggcgttg
   131101 gcagggcaac tcatttctcg tattgcgggc agtgcgattg tcaccgcgcc cgcaaaagcg
   131161 gcaacggatg tactggcaca atttgcgggc gagaagtttc gctttattgc accggatgcc
   131221 ttgttagcca gcgatgagca agccgactgg ctggtggtcg acgaagccgc agccatacct
   131281 gcgccgttgt tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac cactacggtg
   131341 cagggctacg aaggcaccgg acgtggtttt ttgctgaaat tttgcgctcg ctttccgcat
   131401 ttacaccgtt ttgaactgca acagccgatc cgctgggcac agggatgccc gctggaaaaa
   131461 atggttagtg aggcactggt ttttgacgat gaaaacttca cccatacacc acaaggcaat
   131521 atcgtcattt ccgcatttga acaaacgtta tggcgaagcg agccagaaac gccgttaaag
   131581 gtttatcagt tattgtctgg tgcgcactac cggacttcgc cgctggattt acgccggatg
   131641 atggatgcac cagggcaaca ttttttacag gcggctggcg aaaacgagat tgccggagcg
   131701 ctgtggctgg tggatgaggg gggattatct caacaactca gtcaggcggt atgggcaggt
   131761 tttcgtcgcc cgcggggtaa tctggtggcc cagtcgctgg cggcgcacgg tagcaatcca
   131821 ctggcagcga cattgcgtgg acggcgggtc agccggatag cagttcatcc ggcgcgtcag
   131881 cgggaaggca cagggcagca acttattgct ggtgctttgc aatatacgcg tgacctcgac
   131941 tatctttcgg tgagttttgg ttacaccggg gagttatggc gtttctggca ccgctgcggt
   132001 tttgtgctgg tgcggatggg taatcatcgt gaagccagca gcggttgcta tacggcgatg
   132061 gcgctgttac cgatgagtaa tgcgggtaaa caactggctg aacgtgagca ttaccgttta
   132121 cgtcgcgatg cgcaagctct cgcaaagtgg aatggcgaaa cgcttcctgt tgatccacta
   132181 aacgatgccg tcctttctga cgacgactgg cttgaactgg ccggttttgc tttcgctcat
   132241 cgtccgctat taacatcgtt aggttgctta ttgcgtctgc tacaaaccag tgaactggca
   132301 ttaccggcgc tgcgtgggcg tttacagaaa aacgtcagcg acgcgcagtt atgtaccaca
   132361 cttaaacttt caggccgcaa gatgttactg gtccgtcagc gggaagaggc cgcgcaggcg
   132421 ctgttcgcac ttaatgatgt tcgcactgag cgtctgcgcg atcgcataac gcaatggcaa
   132481 ttttttcact gactccttca attttctccc atggtcattg tgagtcagcg gcgtaaaatt
   132541 aatgacatca gttaaggagt tcgccatgaa acatgaccat tttgttgttc aaagcccgga
   132601 taaacctgcg caacagttgt tactgctttt ccacggtgtc ggggataacc cggtggcaat
   132661 gggggaaata ggtagctggt ttgcaccgct gtttcctgat gcgctggtgg tgagtgtcgg
   132721 tggcgcggag ccgagcggta atccggcggg gcgtcagtgg ttttcagtgc agggtattac
   132781 ggaagataat cgccaggcgc gtgtggatgc catcatgccg acgtttattg aaacggtacg
   132841 ctactggcag aaacagagcg gagtaggggc taacgccacg gcgctaattg gtttctcaca
   132901 aggcgcgatt atggcgctgg agagcattaa agccgaaccg ggccttgctt cgcgcgtcat
   132961 tgcctttaac ggacgctatg cgagcctgcc ggagaccgcg tctacagcca cgacaattca
   133021 cttgattcat ggtggtgaag atccggttat cgatctggcg catgcagttg cggcgcagga
   133081 agcgttaatc agtgccggtg gtgacgtgac gctggatatc gtggaggatt taggccatgc
   133141 gatcgacaat cgcagtatgc aatttgccct ggatcacttg cgttatacca ttccgaaaca
   133201 ttatttcgac gaagcgctaa gtggcggtaa gcctggcgat gatgatgtga ttgaaatgat
   133261 gtaaaagaca gattacttgg cctgataaga cgcgtgagcg tcgcatcagg catcgttcac
   133321 cattgccgga tgcggcgtta acgccttatc cggcctacgg ttgagcactc agcttacttc
   133381 ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc acttatcgtt gaaatcacga
   133441 tgcgggggaa gctccggctt gttggcgaga aatttcttat ggtcgacgcg cttgagatct
   133501 ttaatcacgt tcagcaggac gcctaccaaa aagacaatca ccagaatcca ccaatattta
   133561 gccagccagt ccatgcttat ttcctcttgc agaaccactc atcaggcgac gagctgttcc
   133621 atgatacgtt gatacatacg ggcaagtagc tgcaggttgg cagcgttcac acattcatta
   133681 attttatgaa tagtggcatt gaccggcccg agttccacca cctgcgcccc catgcgagca
   133741 ataaagcgcc cgtcggacgt tccgcctgtg gtcagtagct gcggtttaat ttcattatag
   133801 tgctcaaccg cgttaacgac cgcatccacc agtttaccgc gcgcggtcaa aaatggctgc
   133861 ccggaaagcc accagtccac cgtataacgc agttgatgtt tttcaagcag ggcaagcacc
   133921 tgtgctttga tcatctcatc agtcagttcg gtgctgaagc ggaagttaaa ctgcacaaac
   133981 agttcacccg gaataacgtt gttactgccc gttcccgcct gaatattggc aatctgcata
   134041 ctggtcgccg ggaagaattc attgccctga tcccactcaa tagccactaa ttcattaagg
   134101 aaaggtgctg cgcgatgtac cggattgtca gccagatgcg ggtaggcaac atgcccctga
   134161 acgccatgaa tggtgaggtt gcaggttaac gatccgcgac gaccattttt caccacatca
   134221 cctaccactt cgatactcga cggttcgccg accaggcagt aatcgagacg ctcattacgt
   134281 gccattaacg cttcgacgac ttttaccgta ccgttgtgtg cactggcttc ttcatcagag
   134341 gtgatcagaa atgccagtcg ccccgtatgg ttgggatgtt gtgcgacaaa gcgttctgcc
   134401 gctaccacca tcgccgccag cgagcctttc atatctgccg caccgcgccc gaataacatg
   134461 ccatcacgaa tggtgggttc aaacggcgga ttgatccaac gatcggcgtc gccaggcggc
   134521 accacgtcgg tatgcccggc aaaggctaac gtttcacctt gcccacgcca tgcccaaaaa
   134581 ttctgcgtat cggcaaagtc catgcgttca acggtaaaac cgatcgcctg caaacgttca
   134641 atcaacaaag cctggcatcc tgcgtcatca ggactcaggg aagggcggcg aataagctgt
   134701 tgtgtcagct caataaccgg gcacgacata gactacacct catggaaaaa ttgctgataa
   134761 ctggaatcac tgaaacccag cagcataggc ttaccaggcg cgcagagcaa tggacgtttg
   134821 ataattgcag gcatttcagt cattaatgcc gccgcagagg ccgcatcggt gattttattg
   134881 cgggtggttt cgtccagttt acgccaggtt gtaccacggg tgttgagtaa cgcttcccag
   134941 cctaattcgt tgataaaacc gttcaataat tcgctgtcca gcccatcgac gcggtaatca
   135001 tgaaaacgat agtcgatgtt attggcttcc agccaacggc gagccttttt aatggtgtca
   135061 caatttttga tgccgtaaag tgtaaccata atgaatcctt tttcgattta tgagaataaa
   135121 tactcattta agggtgaaaa cattttgcct gcaaatgatt atcagaaaat ataacattat
   135181 cgaagtattc aaattaacaa ctattaaata atattaaaca tttttaacgt aaacattaag
   135241 aatattcatc ttgttatctg tctgtatttt aaccagaatt aaatcagtat tagatgccgt
   135301 atgaatatct cgaaatgttg ctgtgtatca catacaaatt atcaccgcag gaccatagta
   135361 tttacataag gtttgtcccc cggaggtagt catcgcagca atcgagtaaa ttagcttcac
   135421 cacggtatat tgtttccgta gaatacccat aattataaga gaggttgtta tgattgaacg
   135481 tgaactgggg aactggaaag actttatcga agttatgctt cgtaagtaat tcccgagaca
   135541 aagtataaac agcaagaacc cgctgagcag gttcttaatc ggaaagttga aaaaggcgac
   135601 acattggcat gtcgcctttt ttattgctta ttccgggcgc ggcttcagcg ggaagcggcg
   135661 gcgcaccagc acaaagaaca gcggcacgaa gtaaatagcc agaatagtag ctgaaatcat
   135721 cccgcccatt acgccagtac ccaccgcatg ctgaccaccg gaaccggcac ccgtgctggt
   135781 tgccattggc aatacgccga agataaatgc cagcgaggtc atcagaatcg ggcgtaaacg
   135841 ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg cctttttggt tcatctcgtt
   135901 agcaaactcg acgatcagga tggcgttttt cgccgataaa ccaatgaccg ttaacaggcc
   135961 cacctggaag taaacgtcgt tttccagccc gcgcatccag gttgccagca gcgcgccgat
   136021 aacccccagc ggcacgacca gcattaccga gaacggcacc gaccagcttt catacaacgc
   136081 cgccagacac aggaatacca ccagcaagga aatggcgtac agcgccggag cctgcgcgcc
   136141 ggaaagtcgc tcctgatacg acatcgccgt ccactccaga ccaaagccgt ttggcaactg
   136201 cttcactaac gattccataa tatccatcgc cgtaccggta ctgacccccg gtgcggcttc
   136261 cccaacaatc tcaaccgcag aataaccgtt atagcgttcc agacgcggcg agcctgtttc
   136321 ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg ccatctttat ttcggacata
   136381 ccagagatta atgtcatctg gcagcatgcg atacggcgca gctgcctgca catagacttt
   136441 cttcacgcga ccgcgatcca taaagtcatt cacatagctc gaaccccagg cggtttgcag
   136501 tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga gctttacgct ggtcgatatc
   136561 aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc acacgggtta gctccgggtt
   136621 ttccgccgcc agcgccagca actgattacg tgctgccatc agcgcatcgt gacccgctcc
   136681 agcgtggtcc tgcaactcca tatcaaaacc tgcagaacta ccaagaccgc taattgctgg
   136741 cgggctgctg gcgataacgc gagcttcttt aatttggtta aacgccttcg ttgcacgctc
   136801 gataatggca aacgaggtgc cggtcttact gtcgcgttcg ctccagtctt tcaggcggat
   136861 aaacattcgc gccacgtttt gcccgttacc cccggggcca gaaccaacag tggcaaacac
   136921 cgacatgatg ttgtcttttt catgggtgaa gtaatatttc tcgatttgct caacgacttt
   136981 cagggtctgt tgttgggttg aaccgctggg caattgtacc gaggtagtaa acatgccacg
   137041 gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac aggaacacca tgccgccaag
   137101 cagcaggaca taaatcacaa tccagcgcag gctacggtgg agaattttcg ccaccccttt
   137161 ttcgtagcgt tcggcgttgc ggttaaacat ctggttaaac caggcaaaaa agcctttttg
   137221 cccgtgatgc tcaccttttt tcagtggctt aagtagtgtg gcacacagag ccggagtgag
   137281 gatcatcgct accagtactg acagcaccat cgccgcaaca atggtaatag agaactggcg
   137341 atagatggca ccggtggtgc cgccgaagaa ggccattggt acaaataccg ccgaaagaac
   137401 catcgcaatc ccgaccagtg ccccctggat ctgccccatc gatttacgtg tggcttcgcg
   137461 aggagtgagt ccttcctcac tcataatacg ttcgacgttt tccaccacca cgattgcgtc
   137521 atccaccagc agaccgatcg ccagtaccat cgcgaacatg gttaaggtgt tgacgctgta
   137581 accgaaggcg taaagtacgg agaaggttcc catcaacacc agcggcacgg cgatagtggg
   137641 tatcagcgtg gcgcggaagt tttgcaggaa cagatacata acgaggaaaa ccagggcgat
   137701 agcttccagc agcgttttca ccacgtcttc aatggaggct ttaacaaacg aggtggtttc
   137761 atacgccacc ttgtattcca gtccgtgcgg gaagtactgc gccagctcgt cgagacgatt
   137821 gagcaccagc tccgctgtcg ccatttcgtt tgcgccggag gccagtttta cccccagccc
   137881 ggaggctggc ttaccattga agcggctaag ataatcgtat ttctccgccc ccatttcgac
   137941 ggtggcgaca tcgcccagcc ttacctctga gccgtcttga ttcacccgca aggtgatatc
   138001 gcggaactgt tctggtgttt gcagcagtga ctgggcgtta atggtggcgt tgagcgcctg
   138061 cttatcgacg gaaggtgtac caccaagttg cccaaccgca atctgcgcgt tctgtgactc
   138121 aatggcatca gtgacatctt tagccgtcat ctggaaactg ttgagtttcg ccgggtccag
   138181 ccagatacgc atggaatatt gcgaaccata ggcatcgata tccccgacgc cattcacgcg
   138241 gcttaacggg tcctgaatat tactggcaac ataatcggca atatcctgtt tatccatcga
   138301 accatcggta gagacgaaag caatggtcag aatattggta tcgccggttt tacgcaccgt
   138361 cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg gctgattgca gctggttttg
   138421 tacttgctgc acggcttcgt ccggatcggt gcctgcttta aaacttaaag tgacagatgc
   138481 ctgaccggtg ccactgctct gagatgacat atacatgaga ttatcgaggc cggtcatatt
   138541 ttgctcgata acctgggtca cggtgttttc cagcgtctgg gccgatgcgc cgggatagtt
   138601 agcggtcact cgcacattcg gtggcgcgag atcggggtat tgttcaacgg gcaatgaaaa
   138661 aatcgccagg gtccctgtca gacacaacag gattgccagc acccaggcaa aaatggggcg
   138721 atcaataaag aaattcgcca ttaaaagagg acctcgtgtt tctgcgtatc gttgtaggct
   138781 tgacttgctt cactgtagcg gcaaggtacg agccaatcgt ggagaaaaaa aggagttaat
   138841 gtaaattatc atgccctttt atactgaacc ttaagtgcaa gtattctttg gtcagtagga
   138901 ggcattacat caactgactt tcctcaccct ccgcagagcg aaagctaata ctcactaacg
   138961 tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc cagccgttcc gcgcgttcgc
   139021 gcatgatatt cagaccataa tgaccttcgg gttctttcgg ttcaccgata ccaatcccgt
   139081 tatcacggat ataaaccgtg tgattgccgt ccggcgcggt gacgcaactg acggcgattt
   139141 cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc gcgaataatt tgcaacaaat
   139201 gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg gcagtcgagg gtcagtttgg
   139261 cgctggtttg attttgtaac gtatccagca tttccctcaa cgcggagggg agatccgcct
   139321 gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa ctgccgataa gcatcattca
   139381 acgcctggga aaaatcggcc atgatacttt gtgcggtggc gttatcttcc ggtatcgaac
   139441 gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac ctgagccagc gagtcgtgca
   139501 attcgcgggc gatggttgca cgttcttcca tcaacaataa ctgctgaaaa tgcttctgcg
   139561 cctgattaaa gtacaaaccg cgtcccagca tcgatgaaac actgttaagc agcggttctg
   139621 atgatgaaac gtgactattt tgccagtgca gttcgccgta aaccgtctct tgcattgtca
   139681 ccggtaaaat ctgcatcggc aattccgggt ttggttgccc ttcgctaatc cgccagtttt
   139741 caccgacatt taactccaga tattcagccg cttcattgtc gcgaacaatc tgcaaaatat
   139801 ggcggaaaca atgcacatca atctggctgg tgttcagcgc ctgggaacat tgatacaaca
   139861 cctccagacg acgcttggcc tcgtggagat cgcgggtctt ttcttctact gacgcctcca
   139921 gcgaaaggta caatttatgc agctcgctcg acatctggtt aaaggttttt gcaagcagac
   139981 caagctcatt cggcagactg gtatccagcg gcggcgagtc gaactgcccg tgttcaatac
   140041 gttgactggc ggtaaccagc tgattcagcg gggcaaccac ctgatggcgt atacggcgca
   140101 aagtaaaaaa gaccagcgtg aaaataccga taccgccagc cagggaaatc gccaccacca
   140161 gcagcatttt gcgttcagcg tagtgctgta aagccagcac gaacaggtct atctgattaa
   140221 cataattatt aatattggcc tgataccacg gcaaatcgcc cttgctgagc cgattattca
   140281 tctccagcca gttggcattc agatgcgcat agcgagtttt tactgcttct ggcacatacc
   140341 agacgttgag attggttaat accggtgaat gcagtgcctg ctgaaacaac tggcgatgtg
   140401 cattgagttg tggactgcca ctttgcaagt cgtagcccag gcggtaactc tgcatacgta
   140461 gcgatccggc aatattgata gcctcagcgt cgcgcaaact gctcgccaga gttagcagag
   140521 cgatacccgt ggaaagaatc gacagcagca caatgtaaaa aaaggcccgg gccagactgg
   140581 ccgagacggg tcgtttaaca atcacacgcg tcttctccac aaaaaatcat taatgttcca
   140641 gttagctgaa acacaattta gtcagtgatg ggattatttg atctgccaca gtttatagcg
   140701 aaatagttac ttattctggg cacatgaaca ttgccgattc ccctgtaagt cgggtaataa
   140761 caacactcat ataaagaata aggtttttac aaccaaaaaa gaaggtcgtt atgaatcgtt
   140821 ttattatggc caacagtcag caatgtctgg gttgtcatgc ttgtgaaatc gcctgtgtca
   140881 tggctcacaa tgatgagcaa catgtcctga gccaacacca ttttcatccc cgaattacgg
   140941 ttatcaaaca tcaacagcaa cgtagtgcag tgacctgtca ccattgtgaa gatgcgccct
   141001 gcgcccgtag ctgccctaat ggcgcaatca gccacgttga tgacagcatt caggtcaatc
   141061 agcaaaagtg tattggctgt aaatcctgcg tggtggcctg tccttttggt acgatgcaaa
   141121 tcgtcctgac acccgtcgcg gcaggaaaag taaaagccac ggcgcataaa tgcgaccttt
   141181 gtgcggggcg cgaaaacggt cctgcctgtg ttgagaattg cccggcggac gcgctgcaac
   141241 tggtcactga cgtcgcactc tccggcatgg cgaaaccccg ccgcttgcgc accgcgcgtc
   141301 aggaacatca accgtggcat gccagtaccg cggcgcaaga aatgccggta atgagtaaag
   141361 tcgaacaaat gcaggcaacg cccgcgcgtg gcgagccgga taaactggcg attgaagcgc
   141421 gcaaaaccgg ttttgatgaa atttatctgc catttcgcgc cgaccaggca caacgggaag
   141481 cctcgcgctg ccttaagtgc ggcgagcaca gcgtttgtga atggacctgc ccgctgcata
   141541 accatattcc gcagtggatt gaactggtga aagccggaaa catcgacgcc gccgtcgagc
   141601 tttctcacca gaccaacacc ctgccggaaa ttaccggacg cgtttgtccg caagaccgtt
   141661 tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc ggtaactatc ggcaatattg
   141721 aacgctatat ttcagatcag gcgttggcga aaggttggcg tcctgactta agtcatgtca
   141781 ccaaagtgga caagcgggtg gcgattatcg gggcaggtcc ggcagggctg gcctgtgcgg
   141841 atgttctgac ccgcaatggc gtggcggtga cggtgtacga tcgccatcca gaaatcggtg
   141901 gcttgctcac tttcggcatt ccttctttca aactggataa atccctgctg gcacgccgtc
   141961 gggaaatctt cagcgcgatg gggattcact tcgaactcaa ttgtgaagtg ggtaaagatg
   142021 tctctttgga ttcgcttttg gaacaatacg acgcggtctt cgttggcgta ggcacttacc
   142081 gttccatgaa cgcgggttta cccaatgaag atgcgccggg cgtttatgac gcgctgccgt
   142141 tcctcattgc caacactaaa caggtgatgg ggctcgaaga gctaccggaa gagccgttta
   142201 tcaataccgc cggacttaac gtcgtggtac tgggcggcgg cgacaccgcg atggactgtg
   142261 tgcgtactgc gctgcgccac ggcgcgagta acgtcacctg cgcttatcgt cgtgatgaag
   142321 ctaacatgcc aggctcgaag aaagaggtga ggaacgcccg cgaagagggg gccaacttcg
   142381 aatttaacgt ccagccggtg gcgcttgagc tgaatgaaca aggtcacgtc tgcgggattc
   142441 gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg gcgtcggcga cctgtaccga
   142501 ttgagggcag tgaatttgtc atgccagccg atgcggtgat tatggcgttt ggcttcaatc
   142561 cacacgggat gccgtggctg gagtcgcacg gtgtaacggt agacaaatgg ggccgcatca
   142621 tcgcggatgt ggaaagccag taccgttacc agaccaccaa tccgaaaatc ttcgccggtg
   142681 gcgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat ggcagaagga cgtcatgcgg
   142741 cacaggggat tattgactgg ctgggggtaa aatcagtcaa atctcactga tagcctgcgc
   142801 agacaaaccc gacttcacag cgtaagataa ttgttcattt cgcgctgtgg agtcggtatg
   142861 acgcaacaaa tcaccctcat taaagacaaa attctctccg ataactattt caccctgcac
   142921 aacattacct acgatctcac gcgcaaagat ggcgaagtta tccgccataa acgtgaagtt
   142981 tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg cgaagaaaaa gagcgtggtt
   143041 ctgattcgtc agttccgtgt cgctacctgg gttaatggca atgaaagcgg gcagctgatt
   143101 gaaacctgcg ccgggctgct ggataacgac gaaccggaag tgtgcattcg taaagaagcg
   143161 attgaagaga cgggctatga agtgggcgaa gtgcgcaaat tatttgaact gtatatgtcg
   143221 cctggcggtg tgactgagct aatccacttt tttatcgccg aatacagtga caatcagcgc
   143281 gctaacgccg gtggcggtgt tgaagatgaa gatattgaag tgctcgagct gccgttcagc
   143341 caggcgctgg agatgatcaa aaccggcgag atacgtgacg gtaagacggt gttattgctt
   143401 aactatttgc aaacgtcaca tttaatggac tgaaaaataa caataatatt tcgttgttta
   143461 ttattggctc aatccgacaa atctgattga gccgcgctac ctgcgcaacg aagatacgac
   143521 ttgtgctgtt tgtttgaact tctggggtcg taccgtccat gcgctatcgc attttccttc
   143581 tctttttttt cgctttgttg ccgacgtctt tggtgtgggc ggcaccagcg caacgggcgt
   143641 tttccgactg gcaggtcacc tgtaataacc aaaacttctg cgtggcgcgc aatacgggcg
   143701 atcataatgg actggtgatg accctgagcc gcagcgccgg ggcgcatacc gatgccgttt
   143761 tacgtattga gcgcggcggg ttgaagtcgc cggatgcgtc tgaaggagag atagcgccac
   143821 ggctgctgtt agatggtgag ccgttggcgt taagtggtga caagtggcgg atttcaccat
   143881 ggttattagt aaccgatgat acggcaacca tcaccgcgtt tttgcaggtg attcaggaag
   143941 ggaaggcgat caccttacgc gacggcaatc agaccatttc tctgagtggc ttaaaagcgg
   144001 cgttgttgtt tattgatgct cagcaaaagc gcgttggcag tgaaaccgcg tggatcaaga
   144061 aaggggacga accgccgctc agcgtaccgc ccgcgcctgc gctgaaagag gtcgcggtgg
   144121 ttaacccaac gccgacgcca ctctcactcg aagaacgcaa cgatttgctg gattatggaa
   144181 actggcggat gaatggtctg cgctgctcgc ttgatccgtt acgtcgtgag gtgaatgtca
   144241 ctgcgctgac tgatgataaa gcgctgatga tgattagctg tgaggcaggg gcctataaca
   144301 ccattgattt ggcatggatt gtgtcgcgta aaaagccact agcctcgcgc ccggttcggt
   144361 tgcgtttgcc attcaacaac ggtcaggaga cgaatgaact ggaactgatg aacgcaacat
   144421 ttgatgagaa atcgcgtgaa ctggtgacct tagcgaaagg gcgcggatta agcgattgtg
   144481 gcattcaggc gcgctggcgc tttgacggtc agcgttttcg cctggtgcgt tatgccgcag
   144541 aacccacctg cgataactgg catgggccag acgcctggcc cacgttgtgg atcacccgtt
   144601 aaacaaaatt gcggggacat gtaggccgga taagacgctg taggccggat aagacgcgtc
   144661 agcgtcgcat ccggcaatca gcatccggcg atcaccatca ggcacctttc actcccagca
   144721 ccttatgcgc ttttgccaca atattctcgg cggtaaagcc aaagaacggg aacagcttat
   144781 ccgccggagc agattccccg taacccgtca tcccgacaat tacccctttc agaccaacat
   144841 acttgtacca gtaatcggca atacctgctt ccaccgccac gcgagccgca acgttagaag
   144901 gcaacaccga ctcccgatat tcctcatcct gggcgtcgaa aatatcggtc gagggcaggg
   144961 aaactacgcg tacattgcga ccttctcctg ctaatttctc tgccgcttgc agggtaattt
   145021 ccatctctga accggtggca atcagaataa tatctggctt accgccgctg tctttcagca
   145081 cataaccgcc acgagcaatc tctttaacct gatccggcgt acgttccacc tgggccagat
   145141 tctgccttga gaggatcagt gctgtcggcc cgttgtggcg ctcaaccgcc agcttccagc
   145201 ccaccgccgc ttccacctga tcgcacggtc gccaggtgct gaaattcggc gttaagcgca
   145261 gactggccag ttgctcaacg gcctggtgcg tcggaccatc ttcccctaag ccaatagaat
   145321 cgtgggtata aaccataatc tgccgtgctt tcatcagtgc cgccatccgc gcggcattac
   145381 gggcgtattc aacaaacatc aggaacgtcg cggtatacgg cacaaagccg ccgtggtgcg
   145441 caatgccgtt ggcgatagcg gtcatgccaa attcacgcac cccgtagtga atgtagttgc
   145501 ccgccggatc ttccttcagc gacacagagc ctttccagat ggtgaggttg ctgggagcca
   145561 gatccgctga acctccgagc agctccggta gcatcggtcc gtaagcgtta agcgtatttt
   145621 gcgaagcctt acgggtggcg attttcgccg gattcgcctg caactcattg atatatttct
   145681 gagtcgtttt ctcccagtcc ttcggtaaac caccgctcat ccgacgggta aactcttctg
   145741 ccagttgcgg atgagccttt ttataggcgg caaacttctc attccagctc tgctgcgctt
   145801 tttcgccttt ttcgcgggca tcccaggcgt gataaatctt tttagggatc tcaaatggcg
   145861 gatggtgcca gcccagtttt tgccgtgcca gcgccacttc ttcttccccc agtggtgcgc
   145921 cgtgcgcctc ttccttacct gctttattcg gcgaaccaaa gccaatcacc gtacggcaga
   145981 taatcagcga cggcttatct ttcatgcttt gcgcttcaag gatcgcttcc ttcaccgcct
   146041 gcggatcgtg accgtcgatt tcatggatca catgccagtg ataggcttca aaacgttttg
   146101 ccgtatcgtc ggtaaaccag ccttctgttt caccgtcgat ggaaataccg ttgtgatcgt
   146161 aaaaaccaat cagcttgccc agtcccagcg tgcctgccag cgaacagact tcgtgggaaa
   146221 taccttccat caggcagccg tcgcccataa acacataggt gaagtgatcg acgatctcat
   146281 ggtctggctg gttaaactgc gccgccagtg tacgctctgc tatcgccagc ccgacggcgt
   146341 tcgccaaacc ttgtccaagc gggccggtgg tggtttcaac gcctggcgta tagccaatct
   146401 ccgggtggcc tggggttttc gaatgcaact gacggaagtt cttcagttct tccagcggca
   146461 ggtcgtaacc ggtcagatgt agcaaactgt agagcagcat cgacgcgtga ccgttggaaa
   146521 gaataaagcg gtcgcgatca taccaggttg ggtcggtagg attatgttta agaaaatcgt
   146581 tccacagcac ttcggcaata tcagccatgc ccatcggcgc gccgggatga ccagaattgg
   146641 ctttttgtac cgcatccata ctgagtgcgc gaatcgcatt ggcaaggtct tttcgggaca
   146701 tataacactc cgtggctggt ttatagtttg gcggcaagaa gatcttccag tttgcgttga
   146761 tcaacagcga acagacgaat gccttcagac agtttttcta ccgccatcgc atcctgattg
   146821 tgctcccaac ggaactccgc ttcgctcatg ggtgctgggc gtgggaacgt ctgagaaggc
   146881 gggattaatt tacgtaccac tggcgaaact ttttcctgca gctccttcag caaattcggt
   146941 gcgatagtca gtcgatcgca gccggttaag gcgaggattt gttcggtgcg acggaagctc
   147001 gcgcccatca caatggtttc atagtggtgt tgcttatagt agtcgtagat attgcgcacc
   147061 gatttaacgc ccggatcttc ttccaccaca tacgggtcca tcggcttgcg tgcctgatac
   147121 cagtcataaa tacgcccgac aaacggcgaa atcagaaaaa cgcccgcttc tgcacaggcc
   147181 cgcgcctggg caaaagaaaa cagcagcgtc aggttgcagt taataccttc tttttccagc
   147241 tcttcggccg cgcgaattcc ttcccaggtc gaagccagtt tgatcaaaat gcgtgatttc
   147301 tcaacgcctt gttgctgata caagtccacc agatggcgcg ccttttcaat actcttttct
   147361 ttatcaaaag agaggcgtgc atcaacttct gttgacacgc gaccgggtac gattttgaga
   147421 atttcagcac cgaaattgac tgccagtttg tcacacgctg cgaccacctg ttgttcctgg
   147481 gtcttgccat tttttttacc ccaggcgata gcatcgtcta ttaaatgctc atattgtgat
   147541 aatccggcag ctttgagtaa cagcgaagga ttggtggtgg catcctgggg atgataatgg
   147601 cgaatggact caatatcgcc gctgtctgcc acgacagtgg tgaactgttt gatgccgtct
   147661 aactcgttca taggaaatac tccttgaaaa gtaaagtgtt agatgagtgc gttaattcac
   147721 acttctgaga aatttcgcta aacgcatcaa aaaagcatag cagacaggca tggtattgct
   147781 ggattaagca ggtaacatca gtgttatagg attattacca aaacattata ttaatggtga
   147841 gagtttggac ttgctcaaag tctgtagact ccggcagggt aataatgtgc gccacgttgt
   147901 gggcaggggc tgttgcccac acactttatt tgtgaacgtt acgtgaaagg aacaaccaaa
   147961 tggatgacca gttaaaacaa agtgcacttg atttccatga atttccagtt ccagggaaaa
   148021 tccaggtttc tccaaccaag cctctggcaa cgcagcgcga tctggcgctg gcctactcac
   148081 caggcgttgc cgcaccttgt cttgaaatcg aaaaagaccc gttaaaagcc tacaaatata
   148141 ccgcgcgcgg taacctggtg gcggtgatct ctaacggtac ggcggtgctg gggttaggca
   148201 acattggcgc gctggctggc aaaccggtga tggaaggcaa gggcgttctg tttaagaaat
   148261 tcgccgggat tgatgtattt gacattgaag ttgatgaact cgacccggac aaatttattg
   148321 aagttgtcgc tgcgctcgaa ccgaccttcg gcggaatcaa cctcgaagac attaaagcgc
   148381 cagaatgttt ctatattgaa cagaaactgc gcgagcggat gaatattccg gtattccacg
   148441 acgatcagca cggcacggca atcatcagca ctgccgccat cctcaacggc ttgcgcgtgg
   148501 tggagaaaaa catctccgac gtgcggatgg tggtttccgg cgcgggtgcc gcagcaatcg
   148561 cctgtatgaa cctgctggta gcgctgggtc tgcaaaaaca taacatcgtg gtttgcgatt
   148621 caaaaggcgt tatctatcag gggcgtgagc caaacatggc ggaaaccaaa gccgcatatg
   148681 cggtggtgga tgacggcaaa cgtaccctcg atgatgtgat tgaaggcgcg gatattttcc
   148741 ttggatgttc cggcccgaaa gtgctgaccc aggaaatggt gaagaaaatg gctcgtgcgc
   148801 caatgatcct ggcgctggcg aacccggaac cggaaattct gccgccgctg gcgaaagaag
   148861 tgcgtccgga tgccatcatt tgcaccggtc gttctgacta tccaaaccag gtcaacaacg
   148921 tcctttgctt cccgtttatc ttccgtggcg cgctggatgt tggcgcaacg gctatcaatg
   148981 aagagatgaa actggcggcg gtacgcgcga ttgcggaact ggcccacgcc gaacagagcg
   149041 aagtggtggc ttcagcgtat ggcgatcagg atctgagctt tggtccggaa tacatcattc
   149101 caaaaccgtt tgatccgcgc ttgatcgtta agatcgctcc tgcggtcgct aaagccgcga
   149161 tggagtcggg cgtggcgacg cgtccgattg ctgatttcga cgtctacatc gacaagctga
   149221 ctgagttcgt ttacaaaacc aacctgttta tgaagccgat tttctcccag gctcgcaaag
   149281 cgccgaagcg cgttgttctg ccagaagggg aagaggcgcg cgttctgcat gccactcagg
   149341 aactggtaac gctgggactg gcgaaaccga tccttatcgg tcgtccgaac gtgatcgaaa
   149401 tgcgcattca gaaactgggc ttgcagatca aagcgggcgt tgattttgag atcgtcaata
   149461 acgaatccga tccgcgcttt aaagagtact ggaccgaata cttccagatc atgaagcgtc
   149521 gcggtgtcac tcaggaacag gcgcagcggg cgctgatcag taacccgaca gtgatcggcg
   149581 cgatcatggt tcagcgtggg gaagccgatg caatgatctg cggtacggtg ggcgattatc
   149641 atgaacattt tagcgtggtg aaaaatgtct ttggttatcg cgatggcgtt cacaccgcag
   149701 gtgcaatgaa cgcgctgctg ctgccgagtg gtaacacctt tattgccgat acctacgtca
   149761 atgacgaacc agacgcagaa gagctggcgg agatcacctt gatggcggca gaaactgtcc
   149821 gtcgttttgg tattgagccg cgcgttgctc tgttgtcgca ctccaacttt ggttcttctg
   149881 actgcccgtc gtcgagcaaa atgcgtcagg cgctggaact ggtcagggat cgtgcgccag
   149941 atctgatgat tgatggtgaa atgcacggcg atgcagcgct ggtggaagcg attcgcaacg
   150001 accgtatgcc ggacagccct ttgaaaggtt ctgccaatat tctggtgatg ccgaacatgg
   150061 aagctgcccg cattagttac aacttactgc gcgtttccag ctcggaaggt gtgactgtcg
   150121 gtccggtgct gatgggcgtt gcgaaaccgg ttcacgtgtt aacgccgatc gcatcggtgc
   150181 gtcgtatcgt caacatggtg gcgctggccg tggttgaagc gcaaacccaa ccgctgtaat
   150241 tcttttttaa ctctcacgct taccctgaat attcagggta agcggtttgg ctgcaatata
   150301 ttagtaaagc ttattactga gtttgcgaat aataaaaaaa gcagtctata taatatctcg
   150361 atattatttt atttatattc atgcgttgca tatgaaagtt tatgcaccac agcgaatatc
   150421 tctccattct tagtgatcta cctcaccttt taaacgcgct tgccgaattt tgttatttac
   150481 tctgacgaaa aattgtcacg atacacgaaa gtttttcaca ggcggcgact catggataaa
   150541 gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca cactggcgca tctcatcgcg
   150601 catcccggtg aggaactggc gaagaaaatc ggcgttccgg atgcgggcgc aattggcatt
   150661 atgacgctaa ctcccggcga aacggcgatg atcgccggtg atttagcgct gaaagccgct
   150721 gatgtgcata tcggttttct cgaccgcttc agcggtgcat tggtgattta tggctcggtg
   150781 ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt taggtcgctt attaaattat
   150841 acgctttgtg aaatgaccaa aagttaatac gaggcattca tgaaacgaat tgcgtttgtg
   150901 ggtaccgtcg gggcaggaaa aacaacgtta tttaatgcgt tgcaggggaa ttataccctc
   150961 gccagaaaaa cacaggccgt ggaatttaat gataagggcg atattgatac gccgggtgaa
   151021 tattttagcc atccccgctg gtatcacgcc ttaattacca cgctacagga tgtagacatg
   151081 ctgatctatg tccatggcgc aaatgacccg gaaagtcggt tgcctgccgg gttgctggat
   151141 attggcgtca gcaaacggca aatcgccgtc atcagtaaaa cggacatgcc agatgctgac
   151201 gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg aagagccgat tttcgagcta
   151261 aacagccacg acccgcaaag cgtgcagcaa ctggtggatt atctggcatc tctcaccaaa
   151321 caggaggaag caggtgaaaa aactcatcac agcgaatgat attcgtgaag cacacgcgcg
   151381 cggcgaacag gcaatgtccg tggttctgcg cgccagcatt attaccccgg aagcccgtga
   151441 agtggcggat ctactgggtt ttaccatcac cgaatgtgat gaatcgatcc cggtaacggc
   151501 gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc cagcgcatcc gcgaaaccat
   151561 cattgcccag ctgccggaag gccagtttac cgaaagcctg gtcgcacagc tgatggaaaa
   151621 agtgatgaag gaaaaacagt cgctggagca gggcgcaatg cagccgagct tcaaatcggt
   151681 gaccggcaaa ggcggcatca aagtgattga tggcagcagc gtcaagtttg gtcgtttcga
   151741 cggcgcagag ccgcattgcg tgggcttgac tgatcttgtg accggagacg acggcagcag
   151801 catggccgct ggttttatgc agtgggagaa cgcattcttc ccgtggaccc tgaactacga
   151861 cgaaatcgac atggtgctgg aaggggagct gcatgttcgc cacgaaggcg aaaccatgat
   151921 cgccaaagcg ggtgacgtga tgtttatccc gaaaggctcc agtatcgaat ttggtacgac
   151981 atccagcgtg aaattcctgt atgtcgcctg gccggctaac tggcaatccc tatgaaagat
   152041 ttcatcaccg aagcatggct aagagcgaac catacgctca gcgaaggcgc agagatccat
   152101 ctccctgcgg acagtcgcct gacgccctct gcccgggagt tactggaaag ccgccatctg
   152161 cgcatcaagt ttattgacga gcagggccgc ctgtttgttg acgatgaaca gcagcagccg
   152221 cagcccgttc atgggctgac cagtagcgat gaacatccgc aggcgtgctg cgaactgtgc
   152281 cgccagccgg tggcaaaaaa gccggatacg ctgactcacc tgtcggcgga aaaaatggtc
   152341 gccaaaagcg atccgcgtct gggttttcgc gcggttctcg acagcaccat tgcgctggca
   152401 gtgtggctgc aaattgaact ggcggaaccg tggcagccgt ggctggcgga tatccgttcg
   152461 cgtcttggca acattatgcg cgccgatgcg ctgggtgaac cgctgggctg ccaggcgatt
   152521 gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc agccgctgcg ctatctcgac
   152581 cacgatcatc tggtgccgga agccagccac gggcgcgatg cggcgttgct caatctgcta
   152641 cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg tgtttatcac ccgcagcttt
   152701 gaagtattac gcccggacat tttgcaggcg ctgaaccgcc tttccagcac ggtctacgtg
   152761 atgatgatcc tgagcgtaac gaagcagccg ctaacggtga agcagatcca acaacgactg
   152821 ggggaaacgc aatgattatt gaacgttgtc gtgaactggc gttgcgagcg cccgccagag
   152881 tggtttttcc ggatgcgtta gaccaacgtg tgctgaaagc cgcgcaatat ttacatcaac
   152941 aaggtctggc aacgcccatt ctggtcgcca atccgtttga acttcgtcag tttgcgctca
   153001 gtcacggcgt ggcgatggac gggctacagg tgattgatcc gcatggcaac ctcgcaatgc
   153061 gggaagaatt tgctcatcgc tggctggccc gcgcgggcga aaaaacgccg ccggatgcgc
   153121 tggaaaaact taccgaccct ctgatgttcg ccgcagcaat ggtcagcgcc ggtaaagcgg
   153181 atgtctgtat cgcgggcaac ctctcttcca cggcaaatgt gctgcgtgcc ggattacgca
   153241 ttatcggctt gcagccaggc tgtaaaacgc tctcttccat tttcctgatg ttgccacagt
   153301 acagcggccc ggcgttgggc tttgccgatt gcagcgtggt gccacagccg acggcggcgc
   153361 agctggcgga tatcgcgctt gccagtgccg aaacctggcg cgccatcacc ggagaagagc
   153421 cgcgcgtggc gatgctgtcg ttttccagca atggtagcgc ccgtcacccc tgtgttgcta
   153481 acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc aaagctggtg gtggatggcg
   153541 agttacagtt tgacgccgcc ttcgtgccgg aagtggcggc gcaaaaagcg cctgccagcc
   153601 cgctacaggg caaggccaat gtgatggttt ttccgtcgct ggaagccgga aatattggtt
   153661 acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg gccactgata caaggacttg
   153721 ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca ggaaattatc gagctggcgc
   153781 tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga aagcagttta caaacactgg
   153841 ttgaatgaac ggtcccgttc tggaccctga taaagaggaa agcacaatgg aagcattagg
   153901 aatgatcgaa acccggggcc tggttgcact gattgaggct tctgacgcga tggtgaaagc
   153961 tgcgcgcgtc aagctggttg gtgttaagca gattggcggc ggcctgtgta ctgccatggt
   154021 tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca ggtgccgcag cggcacagcg
   154081 tatcggcgag ctggtatctg tacacgttat cccgcgtccg cacggcgacc tggaagaagt
   154141 cttcccgatc ggcctgaaag gcgatagcag caacctgtaa tcaccctgtg tgacgtcgat
   154201 tcgggcgtca cgctctctcc tttttcattt taccttctgc ggagggtagg gggagctttt
   154261 acctgaaata cgccacggag gcgggtatga aactggcagt cgtcactgga caaattgttt
   154321 gtaccgtacg ccatcacgga ctggcgcatg acaaattgct gatggtggaa atgattgatc
   154381 cacaaggtaa tcctgacggg caatgcgccg tcgccatcga caatattggc gcgggaaccg
   154441 gggagtgggt gttactggtg agtggcagtt ccgcccgcca ggcgcataaa agcgaaacgt
   154501 caccggtcga tctgtgcgtg attggcattg tcgatgaggt ggtgtctggc ggtcaggtaa
   154561 ttttccacaa ataaggcaga acatcatgaa tcaacaggat attgaacagg tggtgaaagc
   154621 ggtactgctg aaaatgcaaa gcagtgacac gccgtccgcc gccgttcatg agatgggcgt
   154681 tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc gcccagcaag ggttaaaaag
   154741 cgtggcaatg cgccagttag ccattgctgc cattcgtgaa gcaggcgaaa aacacgccag
   154801 agatttagcg gaacttgccg tcagtgaaac cggcatgggg cgcgttgaag ataaatttgc
   154861 aaaaaacgtc gctcaggcgc gcggcacacc aggcgttgag tgcctctctc cgcaagtgct
   154921 gactggcgac aacggcctga ccctaattga aaacgcaccc tggggcgtgg tggcttcggt
   154981 gacgccttcc actaacccgg cggcaaccgt aattaacaac gccatcagcc tgattgcgcg
   155041 ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa aagtctccca gcgggcgatt
   155101 acgctgctca accaggcgat tgttgccgca ggtgggccgg aaaacttact ggttactgtg
   155161 gcaaatccgg atatcgaaac cgcgcaacgc ttgttcaagt ttccgggtat cggcctgctg
   155221 gtggtaaccg gcggcgaagc ggtagtagaa gcggcgcgta aacacaccaa taaacgtctg
   155281 attgccgcag gcgctggcaa cccgccggta gtggtggatg aaaccgccga cctcgcccgt
   155341 gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca acatcatttg tgccgacgaa
   155401 aaggtactga ttgttgttga tagcgtagcc gatgaactga tgcgtctgat ggaaggccag
   155461 cacgcggtga aactgaccgc agaacaggcg cagcagctgc aaccggtgtt gctgaaaaat
   155521 atcgacgagc gcggaaaagg caccgtcagc cgtgactggg ttggtcgcga tgcggcgaaa
   155581 atcgcggcgg caatcggcct gaacgtgccg caagaaacgc gtttgctgtt tgtggaaacc
   155641 actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc cggtgctgcc cgtcgtgcgc
   155701 gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac tggagggcgg ttgccaccac
   155761 acggcggcaa tgcactcgcg caatatcgaa aacatgaacc agatggcgaa cgccattgat
   155821 accagcattt tcgttaagaa cggac
//
LOCUS       DAZFDP010000002        86992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFDP010000002 DAZFDP010000000
VERSION     DAZFDP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..550
                     /gene="cmtB"
                     /locus_tag="ACHJ1G_000142"
     CDS             107..550
                     /gene="cmtB"
                     /locus_tag="ACHJ1G_000142"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4762064.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            578..1966
                     /gene="cmtA"
                     /locus_tag="ACHJ1G_000143"
     CDS             578..1966
                     /gene="cmtA"
                     /locus_tag="ACHJ1G_000143"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4762065.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1981..3258
                     /gene="yggP"
                     /locus_tag="ACHJ1G_000144"
     CDS             1981..3258
                     /gene="yggP"
                     /locus_tag="ACHJ1G_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4762066.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3255..4220
                     /gene="glpX"
                     /locus_tag="ACHJ1G_000145"
     CDS             3255..4220
                     /gene="glpX"
                     /locus_tag="ACHJ1G_000145"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4762067.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4242..4751
                     /gene="fumE"
                     /locus_tag="ACHJ1G_000146"
     CDS             4242..4751
                     /gene="fumE"
                     /locus_tag="ACHJ1G_000146"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4762068.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4748..5461
                     /locus_tag="ACHJ1G_000147"
     CDS             4748..5461
                     /locus_tag="ACHJ1G_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4762069.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5433..6110)
                     /locus_tag="ACHJ1G_000148"
     CDS             complement(5433..6110)
                     /locus_tag="ACHJ1G_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4762070.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6104..6781)
                     /locus_tag="ACHJ1G_000149"
     CDS             complement(6104..6781)
                     /locus_tag="ACHJ1G_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4762071.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6769..7476)
                     /locus_tag="ACHJ1G_000150"
     CDS             complement(6769..7476)
                     /locus_tag="ACHJ1G_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4762072.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7477..8055)
                     /locus_tag="ACHJ1G_000151"
     CDS             complement(7477..8055)
                     /locus_tag="ACHJ1G_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4762073.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8078..8509)
                     /locus_tag="ACHJ1G_000152"
     CDS             complement(8078..8509)
                     /locus_tag="ACHJ1G_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4762074.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8881..9900
                     /gene="epd"
                     /locus_tag="ACHJ1G_000153"
     CDS             8881..9900
                     /gene="epd"
                     /locus_tag="ACHJ1G_000153"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4762075.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9950..11113
                     /gene="pgk"
                     /locus_tag="ACHJ1G_000154"
     CDS             9950..11113
                     /gene="pgk"
                     /locus_tag="ACHJ1G_000154"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4762076.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11328..12407
                     /gene="fbaA"
                     /locus_tag="ACHJ1G_000155"
     CDS             11328..12407
                     /gene="fbaA"
                     /locus_tag="ACHJ1G_000155"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4762077.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12471..12569)
                     /locus_tag="ACHJ1G_000156"
                     /pseudo
     CDS             complement(12471..12569)
                     /locus_tag="ACHJ1G_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12765..13625
                     /gene="mscS"
                     /locus_tag="ACHJ1G_000157"
     CDS             12765..13625
                     /gene="mscS"
                     /locus_tag="ACHJ1G_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4762078.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13764..14399
                     /gene="argO"
                     /locus_tag="ACHJ1G_000158"
     CDS             13764..14399
                     /gene="argO"
                     /locus_tag="ACHJ1G_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4762079.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14492..15232
                     /gene="yggE"
                     /locus_tag="ACHJ1G_000159"
     CDS             14492..15232
                     /gene="yggE"
                     /locus_tag="ACHJ1G_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4762080.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15399..16295
                     /gene="ygfI"
                     /locus_tag="ACHJ1G_000160"
     CDS             15399..16295
                     /gene="ygfI"
                     /locus_tag="ACHJ1G_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4762081.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16292..17770)
                     /gene="scpC"
                     /locus_tag="ACHJ1G_000161"
     CDS             complement(16292..17770)
                     /gene="scpC"
                     /locus_tag="ACHJ1G_000161"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4762082.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17794..18579)
                     /gene="scpB"
                     /locus_tag="ACHJ1G_000162"
     CDS             complement(17794..18579)
                     /gene="scpB"
                     /locus_tag="ACHJ1G_000162"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4762083.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18590..19585)
                     /gene="meaB"
                     /locus_tag="ACHJ1G_000163"
     CDS             complement(18590..19585)
                     /gene="meaB"
                     /locus_tag="ACHJ1G_000163"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4762084.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19578..21722)
                     /gene="scpA"
                     /locus_tag="ACHJ1G_000164"
     CDS             complement(19578..21722)
                     /gene="scpA"
                     /locus_tag="ACHJ1G_000164"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4762085.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21926..22819)
                     /gene="argP"
                     /locus_tag="ACHJ1G_000165"
     CDS             complement(21926..22819)
                     /gene="argP"
                     /locus_tag="ACHJ1G_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4762086.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22961..23146
                     /locus_tag="ACHJ1G_000166"
     CDS             22961..23146
                     /locus_tag="ACHJ1G_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762087.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23202..23861
                     /gene="rpiA"
                     /locus_tag="ACHJ1G_000167"
     CDS             23202..23861
                     /gene="rpiA"
                     /locus_tag="ACHJ1G_000167"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4762088.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24117..25349
                     /gene="serA"
                     /locus_tag="ACHJ1G_000168"
     CDS             24117..25349
                     /gene="serA"
                     /locus_tag="ACHJ1G_000168"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4762089.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25578..25637
                     /gene="ibsC"
                     /locus_tag="ACHJ1G_000169"
     CDS             25578..25637
                     /gene="ibsC"
                     /locus_tag="ACHJ1G_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4762090.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25970..26029
                     /locus_tag="ACHJ1G_000170"
     CDS             25970..26029
                     /locus_tag="ACHJ1G_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4762091.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26130..26732)
                     /gene="fau"
                     /locus_tag="ACHJ1G_000171"
     CDS             complement(26130..26732)
                     /gene="fau"
                     /locus_tag="ACHJ1G_000171"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4762092.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26753..26936)
                     /gene="ssrS"
                     /locus_tag="ACHJ1G_000172"
     ncRNA           complement(26753..26936)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ1G_000172"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26978..27307)
                     /gene="zapA"
                     /locus_tag="ACHJ1G_000173"
     CDS             complement(26978..27307)
                     /gene="zapA"
                     /locus_tag="ACHJ1G_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4762093.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27475..28053
                     /gene="ygfB"
                     /locus_tag="ACHJ1G_000174"
     CDS             27475..28053
                     /gene="ygfB"
                     /locus_tag="ACHJ1G_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4762094.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28079..29404
                     /gene="pepP"
                     /locus_tag="ACHJ1G_000175"
     CDS             28079..29404
                     /gene="pepP"
                     /locus_tag="ACHJ1G_000175"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4762095.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29401..30579
                     /gene="ubiH"
                     /locus_tag="ACHJ1G_000176"
                     /gene_synonym="visB"
     CDS             29401..30579
                     /gene="ubiH"
                     /locus_tag="ACHJ1G_000176"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4762096.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30602..31804
                     /gene="ubiI"
                     /locus_tag="ACHJ1G_000177"
                     /gene_synonym="visC"
     CDS             30602..31804
                     /gene="ubiI"
                     /locus_tag="ACHJ1G_000177"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4762097.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31878..31961)
                     /locus_tag="ACHJ1G_000178"
     CDS             complement(31878..31961)
                     /locus_tag="ACHJ1G_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762098.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32252..33346
                     /gene="gcvT"
                     /locus_tag="ACHJ1G_000179"
     CDS             32252..33346
                     /gene="gcvT"
                     /locus_tag="ACHJ1G_000179"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4762099.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33370..33759
                     /gene="gcvH"
                     /locus_tag="ACHJ1G_000180"
     CDS             33370..33759
                     /gene="gcvH"
                     /locus_tag="ACHJ1G_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4762100.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33878..36751
                     /gene="gcvP"
                     /locus_tag="ACHJ1G_000181"
     CDS             33878..36751
                     /gene="gcvP"
                     /locus_tag="ACHJ1G_000181"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4762101.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37012..37755
                     /gene="ygfF"
                     /locus_tag="ACHJ1G_000182"
     CDS             37012..37755
                     /gene="ygfF"
                     /locus_tag="ACHJ1G_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4762102.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37812..39245)
                     /gene="bglA"
                     /locus_tag="ACHJ1G_000183"
     CDS             complement(37812..39245)
                     /gene="bglA"
                     /locus_tag="ACHJ1G_000183"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4762103.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39290..39601
                     /gene="yqfB"
                     /locus_tag="ACHJ1G_000184"
     CDS             39290..39601
                     /gene="yqfB"
                     /locus_tag="ACHJ1G_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4762104.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39765..40424
                     /gene="yqfA"
                     /locus_tag="ACHJ1G_000185"
     CDS             39765..40424
                     /gene="yqfA"
                     /locus_tag="ACHJ1G_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4762105.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40620..41600)
                     /gene="ygfZ"
                     /locus_tag="ACHJ1G_000186"
     CDS             complement(40620..41600)
                     /gene="ygfZ"
                     /locus_tag="ACHJ1G_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4762106.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41843..42109
                     /gene="sdhE"
                     /locus_tag="ACHJ1G_000187"
     CDS             41843..42109
                     /gene="sdhE"
                     /locus_tag="ACHJ1G_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4762107.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42090..42497
                     /gene="ygfX"
                     /locus_tag="ACHJ1G_000188"
     CDS             42090..42497
                     /gene="ygfX"
                     /locus_tag="ACHJ1G_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4762108.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42537..43058)
                     /gene="fldB"
                     /locus_tag="ACHJ1G_000189"
     CDS             complement(42537..43058)
                     /gene="fldB"
                     /locus_tag="ACHJ1G_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4762109.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43170..44066
                     /gene="xerD"
                     /locus_tag="ACHJ1G_000190"
     CDS             43170..44066
                     /gene="xerD"
                     /locus_tag="ACHJ1G_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4762110.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44091..44801
                     /gene="dsbC"
                     /locus_tag="ACHJ1G_000191"
     CDS             44091..44801
                     /gene="dsbC"
                     /locus_tag="ACHJ1G_000191"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4762111.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44807..46540
                     /gene="recJ"
                     /locus_tag="ACHJ1G_000192"
     CDS             44807..46540
                     /gene="recJ"
                     /locus_tag="ACHJ1G_000192"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4762112.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46631..47729
                     /gene="prfB"
                     /locus_tag="ACHJ1G_000193"
     CDS             join(46631..46705,46707..47729)
                     /gene="prfB"
                     /locus_tag="ACHJ1G_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4762113.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47739..49256
                     /gene="lysS"
                     /locus_tag="ACHJ1G_000194"
     CDS             47739..49256
                     /gene="lysS"
                     /locus_tag="ACHJ1G_000194"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4762114.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49299..49847)
                     /gene="idi"
                     /locus_tag="ACHJ1G_000195"
     CDS             complement(49299..49847)
                     /gene="idi"
                     /locus_tag="ACHJ1G_000195"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4762115.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            49902..49973
                     /locus_tag="ACHJ1G_000196"
     CDS             49902..49973
                     /locus_tag="ACHJ1G_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762116.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49970..50095)
                     /gene="yqfG"
                     /locus_tag="ACHJ1G_000197"
     CDS             complement(49970..50095)
                     /gene="yqfG"
                     /locus_tag="ACHJ1G_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4762117.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50097..51545)
                     /gene="uacT"
                     /locus_tag="ACHJ1G_000198"
     CDS             complement(50097..51545)
                     /gene="uacT"
                     /locus_tag="ACHJ1G_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4762118.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIHLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51981..53900
                     /gene="ygfT"
                     /locus_tag="ACHJ1G_000199"
     CDS             51981..53900
                     /gene="ygfT"
                     /locus_tag="ACHJ1G_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4762119.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53900..54388
                     /gene="ygfS"
                     /locus_tag="ACHJ1G_000200"
     CDS             53900..54388
                     /gene="ygfS"
                     /locus_tag="ACHJ1G_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4762120.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54424..55791)
                     /gene="ghxQ"
                     /locus_tag="ACHJ1G_000201"
                     /gene_synonym="ygfQ"
     CDS             complement(54424..55791)
                     /gene="ghxQ"
                     /locus_tag="ACHJ1G_000201"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4762121.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55827..57143)
                     /gene="guaD"
                     /locus_tag="ACHJ1G_000202"
     CDS             complement(55827..57143)
                     /gene="guaD"
                     /locus_tag="ACHJ1G_000202"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4762122.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57161..58561)
                     /gene="xanQ"
                     /locus_tag="ACHJ1G_000203"
     CDS             complement(57161..58561)
                     /gene="xanQ"
                     /locus_tag="ACHJ1G_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4762123.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58726..61596)
                     /gene="xdhD"
                     /locus_tag="ACHJ1G_000204"
     CDS             complement(58726..61596)
                     /gene="xdhD"
                     /locus_tag="ACHJ1G_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4762124.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61593..62372)
                     /gene="ygfM"
                     /locus_tag="ACHJ1G_000205"
     CDS             complement(61593..62372)
                     /gene="ygfM"
                     /locus_tag="ACHJ1G_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4762125.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62423..63751)
                     /gene="ssnA"
                     /locus_tag="ACHJ1G_000206"
     CDS             complement(62423..63751)
                     /gene="ssnA"
                     /locus_tag="ACHJ1G_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4762126.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63754..66852)
                     /gene="ygfK"
                     /locus_tag="ACHJ1G_000207"
     CDS             complement(63754..66852)
                     /gene="ygfK"
                     /locus_tag="ACHJ1G_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4762127.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67174..67752)
                     /gene="mocA"
                     /locus_tag="ACHJ1G_000208"
     CDS             complement(67174..67752)
                     /gene="mocA"
                     /locus_tag="ACHJ1G_000208"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4762128.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            67855..68625
                     /gene="yqeC"
                     /locus_tag="ACHJ1G_000209"
     CDS             67855..68625
                     /gene="yqeC"
                     /locus_tag="ACHJ1G_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4762129.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68673..70298
                     /gene="yqeB"
                     /locus_tag="ACHJ1G_000210"
     CDS             68673..70298
                     /gene="yqeB"
                     /locus_tag="ACHJ1G_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4762130.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70339..71271)
                     /gene="arcC"
                     /locus_tag="ACHJ1G_000211"
     CDS             complement(70339..71271)
                     /gene="arcC"
                     /locus_tag="ACHJ1G_000211"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4762131.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71319..72704)
                     /gene="hyuA"
                     /locus_tag="ACHJ1G_000212"
     CDS             complement(71319..72704)
                     /gene="hyuA"
                     /locus_tag="ACHJ1G_000212"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4762132.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72757..73968)
                     /gene="ygeY"
                     /locus_tag="ACHJ1G_000213"
     CDS             complement(72757..73968)
                     /gene="ygeY"
                     /locus_tag="ACHJ1G_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4762133.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(74026..75222)
                     /gene="dpaL"
                     /locus_tag="ACHJ1G_000214"
     CDS             complement(74026..75222)
                     /gene="dpaL"
                     /locus_tag="ACHJ1G_000214"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4762134.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75280..76467)
                     /gene="ygeW"
                     /locus_tag="ACHJ1G_000215"
     CDS             complement(75280..76467)
                     /gene="ygeW"
                     /locus_tag="ACHJ1G_000215"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4762135.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76946..78724
                     /gene="ygeV"
                     /locus_tag="ACHJ1G_000216"
     CDS             76946..78724
                     /gene="ygeV"
                     /locus_tag="ACHJ1G_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4762136.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(78764..79243)
                     /gene="xdhC"
                     /locus_tag="ACHJ1G_000217"
     CDS             complement(78764..79243)
                     /gene="xdhC"
                     /locus_tag="ACHJ1G_000217"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4762137.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(79240..80118)
                     /gene="xdhB"
                     /locus_tag="ACHJ1G_000218"
     CDS             complement(79240..80118)
                     /gene="xdhB"
                     /locus_tag="ACHJ1G_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4762138.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(80129..82426)
                     /gene="xdhA"
                     /locus_tag="ACHJ1G_000219"
     CDS             complement(80129..82426)
                     /gene="xdhA"
                     /locus_tag="ACHJ1G_000219"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4762139.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            82841..83596
                     /gene="actS"
                     /locus_tag="ACHJ1G_000220"
     CDS             82841..83596
                     /gene="actS"
                     /locus_tag="ACHJ1G_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4762140.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            83675..83748
                     /locus_tag="ACHJ1G_000221"
     tRNA            83675..83748
                     /locus_tag="ACHJ1G_000221"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83707..83709,aa:Gly,seq:ccc)
     gene            84195..84452
                     /locus_tag="ACHJ1G_000222"
     CDS             84195..84452
                     /locus_tag="ACHJ1G_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762141.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(84486..84806)
                     /gene="yecR"
                     /locus_tag="ACHJ1G_000223"
     CDS             complement(84486..84806)
                     /gene="yecR"
                     /locus_tag="ACHJ1G_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HFO4762142.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQCTG
                     DIKK"
     gene            complement(85459..86010)
                     /locus_tag="ACHJ1G_000224"
     CDS             complement(85459..86010)
                     /locus_tag="ACHJ1G_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4762143.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVALDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIAAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
BASE COUNT        21182 a        21467 c        22886 g        21457 t
ORIGIN      
        1 ctgttttcaa aacatcggtt tccctgctca attgctttca ttaaaccgca cgagtatttt
       61 atttttaccg ccggacggta ccctaaaaat aaaataagga taatttatgc ggcttagtga
      121 ttattttcca gaatcatcaa tctcagttat acattcagca aaagattggc aggaagctat
      181 cgatttctcg atggtatcat tgctggataa aaactatatc agcgagaatt acattcaggc
      241 aattaaagat tccaccatta acaatggccc ttattatatt ctcgcaccag gcgtggcaat
      301 gcctcatgcg cgaccggaat gtggggcgct taaaaccggg atgtctttga cattacttga
      361 acaaggtgtt tattttccgg ggaatgacga accaattaaa ttactcatcg gactctctgc
      421 tgccgatgcc gattcgcaca ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga
      481 agaaatactc gaacaactct taacagcatc atcagaaaaa caattagcgg acattatcag
      541 ccgcggataa ttactctctc ctctacagga taatattatg gaaaacaagt ctgctcgtgc
      601 aaaggtccag gcttttgggg gctttttgac tgcaatggtc atccccaata ttggtgcttt
      661 tattgcctgg ggttttatta ctgcgttatt tattcccacc ggttggctgc ctaacgaaca
      721 tttcgccaaa attgtcggcc cgatgattac ctatttattg cccgtgatga ttggttctac
      781 aggtggtcat ctggtcggcg gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg
      841 tgtgatcgtt ggcgcagaga tcccgatgtt ccttggctca atgatcatgg ggccgctcgg
      901 tgggttggtc ataaaatatg tcgataaggc actggaaaaa cgcatacctg ccggttttga
      961 gatggttatc aataacttct cattaggtat cgcggggatg ctcctttgtc tgctgggttt
     1021 tgaagtgatc ggcccggcgg tgttaattgc caatactttc gtcaaagagt gtatcgaggc
     1081 gctggtacat gcgggttatc tgcctctgct gtcagtcatc aatgaaccgg cgaaagtgct
     1141 tttccttaat aatgcgatcg atcagggcgt ctattatccg ctgggaatgc aacaggcttc
     1201 ggttaacggt aaatccatct tctttatggt ggcctctaac ccaggtccgg gcctggggct
     1261 gctgctggcg tttaccttgt ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat
     1321 gattattcac ttcctcggtg ggatccacga actgtatttc ccatatgtgc tgatgaagcc
     1381 gctgactatt attgccatga ttgcgggcgg tatgtctggt acctggatgt ttaacttact
     1441 ggacggtggt ctggtggctg gcccaagtcc ggggtctatc tttgcttacc tggcactgac
     1501 gccgaaaggc tcgttcctgg cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt
     1561 tgctattact tcgctgatac tgaagatgga aaaaacggtg gaaacggaga gcgaagatga
     1621 gtttgctcag tcagccaatg cggttaaggc gatgaaacaa gagggtgcat tctcgttaag
     1681 cagggttaag cgtatcgcct ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc
     1741 gaccaccttc cgtaaacgcc tggaaaaagc ggggctggca atagaagtaa aacattacgc
     1801 gatagaaaac gtgcctgcgg atgccgatat cgtcgtcact cacgccagtc tggaagggcg
     1861 cgtgaaacgt gtgacggata aaccgctgat attgattaat aactatattg gcgatccaaa
     1921 actcgacact ttatttaatc aattaaccgc cgaacataaa cactgattac agaggtaaaa
     1981 atgaaaacca aagttgctgc tatttatggc aagcgggatg tccgtctgcg cgaatttgaa
     2041 ctgccagaaa ttaccgataa tgaactgtta gtgagtgtaa tttctgacag cgtctgttta
     2101 tcgacctgga aagcggcgtt actcggtagt gaacataaac gcgtacccga cgatttagaa
     2161 aatcatccgg tcattaccgg gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat
     2221 ctcactggca aatataaaaa aggccagcgt tttgtattgc aaccggcgat ggggttacca
     2281 agcggatatt cagcaggcta cagctacgaa tattttggcg gtaacgccac ctatatgatt
     2341 attcccgaaa tcgccattaa tttgggctgc gtattaccgt atcacggctc ttattttgct
     2401 gcggcgtcgc tggcagagcc tatgtgctgc attattggtg cttatcatgc caattatcac
     2461 accacgcaat atgtttatga gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg
     2521 ctggcgtgtg cgggtccgat gggcattggc gctatcgatt acgccattaa cggtggcata
     2581 caaccgtcac gggtggtggt ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag
     2641 ctgctgccgg tggatctggc ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa
     2701 gggatgagcg atccggtcca gacgctgcgg gcgctgacag gagatgtcgg gttcgatgac
     2761 atttttgttt atgcggcggt gcctgctgtc gttgagatgg ctgacgaatt actggcggaa
     2821 gatggctgtc tgaacttctt tgccgggccg acggataaaa acttcaaagt gccgtttaat
     2881 ttctacaacg tccattacaa tagcacgcac gtagtcggta catccggcgg ttcaacggac
     2941 gacatgaaag aggcgattgc gttaagcgcc acggggcagt tgcagccgtc ctttatggtc
     3001 acgcatatcg gtgggctgga tgcggtgcca gataccgtgc tcaatctgcc ggatatccct
     3061 ggcggtaaaa aactcattta taacggcgtg accatgccgc tcactgccat tgccgatttt
     3121 gccgaaaaag gcaaaaccga tccgctgttt aaagagttgg cgcggctggt tgaggaaacg
     3181 cacggcatct ggaatgaaca ggccgagaaa tatctgctgg cacaatttgg cgttgatatc
     3241 ggggaggccg cgcaatgatg tccctggcgt ggccattatt tcgcgttacg gaacaggcag
     3301 cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa taaaattgat ggcctggcgg
     3361 tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg
     3421 aaggagaaat tgaccatgcg ccgatgctgt ggattggcga agaggtgggc aaaggcgatg
     3481 ggccagaagt cgatatcgcg gttgacccga ttgaaggcac gcggatggta gcgatggggc
     3541 agagcaatgc gctggcggtc atggctttcg cccctcgaga tagcctgtta catgcgcccg
     3601 atatgtatat gaaaaagctg gttgttaatc gactggctgc cggggcgatt gatctgtctt
     3661 tgcctctggc ggacaacttg cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc
     3721 tgcgcatggt tacgctggat aaaccgcgcc tgagtgccgc gattgaagaa gcaacacagc
     3781 tgggcgtgaa ggttttcgct ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct
     3841 ggcaggataa tccttacgac gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt
     3901 ctgcctgtgc cgttaaagca ttaggtgggg atatgcaggc cgagctgatt gacttttgtc
     3961 aggctaaagg ggattacacg gaaaatcggc agattgcaga gcaggagcgc aagcgttgta
     4021 aggcaatggg cgttgacgtt aaccgtgtgt actcgctcga tgaactggtg agggggaacg
     4081 atatcctctt tagcgctacg ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga
     4141 cggcgaatgg ggtgcggacg cagacattac tgatcggcgg cgcggaccaa acgtgtaata
     4201 taatagactc cctgcattga tggtgatcta tcggaagacc aatggcgacg ctgacagaag
     4261 atgatgtgct tgagcaactg gatgcacagg acaatttatt ttcatttatg aaaactgcgc
     4321 attctatttt gctccagggg atacgccagt ttctgccgtc gctgtttgtc gataacgatg
     4381 aagagatcgt cgaatatgca gtgaagccgt tactcgccca aagcggcccg cttgacgata
     4441 ttgatgttgc gctgcgtttg atttatgcgc tggggaaaat ggataaatgg ctgtatgccg
     4501 atatcacgca cttttcccag tactggcatt acctgaacga acaggatgaa acgcccggat
     4561 ttgccgatga catgacctgg gattttatca gcaacgtcaa tagcataacc tgcaatgcga
     4621 cgctctatga cgcgttaaaa gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc
     4681 gttttagcgg aatggtcaaa acggcgctga cgctggcagt aacgaccacc ttaaaggaat
     4741 taacgccgtg aaaattgaat taacggtgaa tgggctgaac gtgcaagcgc agtaccatga
     4801 tgaagagata gaacgcgtgc ataaaccgtt gctgcgtatg ctggcggcct tacagacggt
     4861 aagtccgcag cgacgtacgg tggtttttct ttgcgctccg ccggggacgg gtaaatctac
     4921 cttaactacc ttctgggaat atctcgcgca acaagaccct gaattacccg ctatccagac
     4981 gctcccgatg gatggttttc accattacaa tagctggctg gatgcgcatc aattgcgccc
     5041 cttcaaaggc gcaccagaga catttgacgt tgcgaaactg gcggaaaatc tgtgccaggt
     5101 agtggaaggg gattgtacgt ggccgcagta tgatcgacaa aagcatgatc ctgttgaaga
     5161 tgcgttgcac gttaccgcac cactcgtcat cgtcgaagga aactggttgt tgctggacga
     5221 cgagaagtgg tgccagcttg cgcaattttg tgatttctcg atctttatca acgcgccagc
     5281 tactgcgttg cgggaacgac tggtagggcg caagctggcg ggaggggtat cgctggcaga
     5341 cgcagaagct ttttatgacc gtaccgacgg gccgaatgtt cgccgggggc tggaagagag
     5401 tctgcctgca aatttgacct taatgatgac cgctacgggt gaatatcgtc tggtggatta
     5461 acgttcctga ttagaccatc ttccagtcgc accacacgag agaaatgacg ccgggtaaat
     5521 gcggcgtcat gactgattgc tacgacgctc gtgcctcgct ggccgcactt ctccagccag
     5581 ctttcaaaca cgctcaacca attttcgtca aaatcccgac tcggctcgtc cagcagcaat
     5641 agtggcggag agagtgcttc caggcaggcg acggcgacca tccgtcgctg cgcactatgc
     5701 agatccaacg ggtgcgagtt ggcagtgtct gctaactgac aacattgcag ggcagcatgg
     5761 gtacgttgag tgatttcatc cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg
     5821 tcagcgacgg tgctgtgaaa aagttggttt tccgcttcct gaaacagtac gccaaccttt
     5881 gccgcgcgct ggcggttttt caggtttttc atcgcctgtt gttgcagcat aaccgtaccg
     5941 gctgtgggcg taagaagacc tgccattact cgtaacagcg tcgattttcc cgcgccattg
     6001 tcgccggtca gcgccaacca ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa
     6061 tccgttgccg cgccaggcca gcgataagat atcttattta acgttaacat agtggctgga
     6121 gcgctccgtt ttgcaattgc cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac
     6181 gttcaaaaag aataatcagc gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca
     6241 gcatttcgct ggcctgcgga gtcaggcggc taaaggcttc atcaagaatt aagagctttg
     6301 gctgcatcgc gatggcgcag gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg
     6361 ccgggtgacg atggcgcaac ggctggcact cggtcagagt cagggcggca tcaatccgcg
     6421 ccataatctc tgcttcagca agacacagat tttctggccc aaaggcaact tcttcttcca
     6481 cgctaaaggt acagccggag agctgcaaat agggcgattg ctggacgagc tggatggtgg
     6541 cggattgttc attaagcggg agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg
     6601 ttatctctcc tggcagaaag tccggatacc agcccgccat cagctgtgcc agcgtacttt
     6661 tgccactgcc gttgtcacca aagatggcga ccatccccgg tttgacatag tgaaagtcat
     6721 aacagaacgg aggatgcgtt gagtgtgtcg ggcaataacg gaactgttct aacgtaacca
     6781 tatccacgct ccgaattctg tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg
     6841 tagggtgcta tcggctggtg cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg
     6901 catatccagc gccgcaccac ggacagccag atcgttaagc gcattgtgtg ttagcggaat
     6961 aattaacgcg ggcatcgcac gtaaacgctg ataccagccc tcatccagcg gcacgccgcg
     7021 ggcacgctgc gcttcatgaa taatcgctaa ttgccgtttt aactgctcaa cgaccaataa
     7081 cggcccggca aacagataag cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat
     7141 aaaacattgc acggggacgt actgcatcca caactgtgag gttgaaacaa tcgccagaat
     7201 ccgcaaccag agcgtaatag catgcgacca ccgttctggc gagcgtggcg tgccgcttag
     7261 ccattctggt agccagccac cgtgtaccag ccacagcccg gctcccagcg agaacatcag
     7321 ccagacgaca tattttgccc gccgctgcgt ggcctttagc gcaataaggc agaaaaaggt
     7381 cgccgcgcta tagatcggca aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc
     7441 caatgcccac agtgttaatg aggtaaatgg atgcatttag cgcacggcag ccattgccgg
     7501 aaaatggcgc gtggtgcgaa tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt
     7561 aagaatttta tcgaccaggt tcgcgcctat caccgtaatc gccaccgact ccaccaggtt
     7621 ttgccccatc gagtgcatcc aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc
     7681 gaacagcgca gtacgcagcg gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc
     7741 gctgacaacg acttttggca gggtgcgaaa ccaacccgct cgcgccaggc aaccggcgac
     7801 caggccgatg accatcgcga cgggtgcaaa tgcggcggcg atcgggttgg tcagcaatcc
     7861 ccagagtaaa ttggtcagta agccggtcag cattccgatc accggaccaa gcagtaccgc
     7921 gctaataagt gtgccgatgg agtcgagaaa tataggtaat ttgaccatgc tggccagttg
     7981 cccgccgatc atattgatgg cgatagatat aacgatcaac accagtgcct gactggaaaa
     8041 gtggctacgc gccataagaa aacctctttg aaagtagtta tttaacgggg gaaatatgca
     8101 actcgtcgta accagaaagc gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac
     8161 cgatcaccag ttcaagcgtg gtccgcacgg tacagccggg catcatatga ccgccaagca
     8221 tcgtgccgtg tggatcggaa acgcagagat gaagatgctc accactctgt tccagcgtgc
     8281 cgtttagtga gatcacttcg aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat
     8341 agcgcaaggc gacgtcagtc aggctaccgg tacagcctgc aatccaggcg gcatgaagtt
     8401 gatgctgctg tgcaaaagcg tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca
     8461 gtgcgtaaaa gcgtgcggag gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc
     8521 gaaactcgga cgtattgcgg ataatatcga cgctgcatca gtcatattct gatataagtc
     8581 agggtttgtg acggcacaac gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg
     8641 gaataaattc cacaggggcg gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt
     8701 gactggacaa tcattccttt tattccacgt ttcgcttatc ctagctgaag cgtttcagtc
     8761 gattaaatgt tcgacaatta accaatcagt cgcagtttgc gacaggtaag gtttccccgg
     8821 acgatttgct ggattactct gtctggcaaa tttgctcagg gaaaaccttg caggagatct
     8881 atgaccgtac gcgtagcgat aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct
     8941 ttgtatgaat ccggacgccg ggcggaaatt accgtggtgg caatcaacga actggcggat
     9001 gctgcgggca tggcgcattt gttgaaatat gacaccagcc atggccgttt tgcatgggaa
     9061 gtacgacagg aacgcgatca actttttgtt ggtgatgacg ccatccgcgt attgcatgaa
     9121 cgttcactgc aatcgctccc ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc
     9181 ggcgtatatg gctcccgcga gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg
     9241 ctcttttcac atcctggcag taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag
     9301 gatcaacttc gtgcggaaca ccgcatcgtt tctaacgctt cctgtaccac gaattgcata
     9361 attcccgtca tcaaattgtt agatgatgcg tacggtattg agtccggcac tgtgaccaca
     9421 attcactccg ccatgcacga tcaacaggtt attgatgcat accatcctga cctgcgtcgc
     9481 acccgggcag ccagccagtc gatcattccg gtcgatacta aactggccgc cggtatcaca
     9541 cgattttttc cgcaatttaa cgatcgcttt gaagcgattg cggtacgtgt gccaaccata
     9601 aatgtgacgg caatcgattt aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc
     9661 aacctgttgc tgcaaaaagc agcacaaggt gcatttcatg gtatagttga ctatacggaa
     9721 ttgccgttgg tctctgtaga ttttaaccac gatccgcaca gtgccattgt cgatggcacc
     9781 caaacccggg tcagtggcgc acacctgatc aaaacgttgg tctggtgcga taacgaatgg
     9841 ggctttgcta accgaatgct cgacacgacg ttagctatgg ctactgttgc tttcaggtaa
     9901 gacgcaagca gcgtctgcaa aacttttaga atcaacgaga ggattcacca tgtctgtaat
     9961 taagatgacc gatctggatc ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt
    10021 accagtaaaa gacgggaaag taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat
    10081 cgaactggcc ctgaaacaag gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac
    10141 cgaaggcgag tacaacgaag aattctctct gctgccggtt gttaactacc tgaaagacaa
    10201 actgtctaac ccggttcgcc tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg
    10261 tgaactggtt gttctggaaa acgttcgctt caacaaaggc gagaagaaag acgacgaaac
    10321 cctgtccaaa aaatacgctg cactgtgtga cgtgttcgta atggacgcat tcggtactgc
    10381 tcaccgcgcg caggcttcta ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg
    10441 cccgctgctg gcagctgaac tggacgcgct gggtaaagca ctgaaagaac cagctcgccc
    10501 gatggtggct atcgttggtg gttctaaagt atctaccaaa ctgaccgttc tggactcctt
    10561 gtctaaaatc gctgaccagc tgattgttgg tggtggtatc gctaacacct ttatcgcggc
    10621 acaaggccac gatgtgggta aatccctgta cgaagctgac ctggttgacg aagctaaacg
    10681 tctgctgacc acctgcaaca tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc
    10741 tgaaactgca ccggctaccc tgaaatctgt taacgatgtg aaagctgacg agcagatcct
    10801 ggatatcggt gatgcttccg ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat
    10861 tctgtggaac ggtccggttg gcgtgttcga attcccgaac ttccgcaaag gtactgaaat
    10921 cgtggctaac gctatcgcag acagcgaagc gttctccatc gctggcggcg gcgacactct
    10981 ggcagcaatc gacctgttcg gcattgctga caaaatctcc tacatctcca ctggcggcgg
    11041 cgcattcctc gaattcgtgg aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg
    11101 cgctaagaag taaaaaatca cagggcaggg aaacctgccc ttgtttcagc gcgcttttag
    11161 agcacgcatt ttttcatact ctaaataatt cgagttgcag gaaggcgaca agagagtgaa
    11221 tccccaggag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    11281 acttgaagta tgatgagtat aaggcccgac gatacaggac aagagacatg tctaagattt
    11341 ttgatttcgt aaaacctggc gtaatcactg gtgatgacgt acagaaagtt ttccaggtag
    11401 caaaagaaaa caacttcgca ctgccagcag taaactgcgt cggtactgac tccatcaacg
    11461 ccgtactgga aactgctgct aaagttaaag cgccggttat cgttcagttc tccaacggtg
    11521 gtgcttcctt tatcgctggt aaaggcgtga aatctgacgt tccgcagggt gctgctatcc
    11581 tgggcgcgat ctctggtgcg catcacgttc accagatggc tgaacattat ggtgttccgg
    11641 ttatcctgca cactgaccac tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg
    11701 acgcgggtga aaaacacttc gcagctaccg gtaagccgct gttctcttct cacatgatcg
    11761 acctgtctga agaatctctg caagagaaca tcgaaatctg ctctaaatac ctggagcgta
    11821 tgtccaaaat cggcatgact ctggaaatcg aactgggttg caccggtggt gaagaagacg
    11881 gcgtggacaa cagccacatg gacgcttctg cactgtacac ccagccggaa gacgttgatt
    11941 acgcatacac cgagctgagc aaaatcagcc cgcgtttcac catcgctgcg tccttcggta
    12001 acgtacacgg tgtttacaag ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt
    12061 ctcaggaata tgtttctaag aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc
    12121 acggtggttc cggttctact gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga
    12181 aaatgaacat cgataccgat acccaatggg caacctggga aggcgttctg aactactaca
    12241 aagcgaacga agcttatctg cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga
    12301 acaagaaata ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc
    12361 gtctggaaaa agcattccag gaactgaacg cgatcgacgt tctgtaagtc attcctttct
    12421 gcttatctca aggcccgctc tgcgggtctt tttttcgcca aaagcaaaat tgcctgatgc
    12481 gctacgctta tcaggcctac acgtatcctg caatatattg aatttgcagg tttttgtagg
    12541 ccggataagg cgttcacgcc gcatccggca tgacaaagcg cacattgata tctgtctgag
    12601 ccccgctctg cggggatttt ttcgccaaaa gcaaggtgat tcagatgaga aatctgtgat
    12661 ctatttggca aaattatgct ttattgttta cccttgtcag actgcccgtc ataaggcggc
    12721 ggagtgtatt tctccatttt gagtcagttg aaaaggaata ttgaatggaa gatttgaatg
    12781 ttgtcgatag cataaacggc gcgggaagct ggctggtagc taaccaggcg ctgctgctaa
    12841 gttatgcagt aaacatcgtg gcggcactcg cgatcatcat cgttggtttg attatcgcgc
    12901 ggatgatttc caacgcggtg aatcgcctga tgatctcccg taaaatcgat gccactgttg
    12961 ctgattttct ttctgcatta gtccgttacg gtattatcgc ctttacgcta atcgcagccc
    13021 tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt actcggtgcc gcaggcttag
    13081 ctgttggtct ggctttgcag gggtcacttt ctaacctggc cgctggcgtg ttacttgtca
    13141 tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc ggtactgtgc
    13201 tgagtgtgca gattttctcc accaccatgc gtactgcaga cggtaaaatt atcgttattc
    13261 cgaacggtaa aattattgcc ggaaatatta ttaacttctc ccgcgagcca gttcgccgta
    13321 acgaatttat tattggcgtg gcgtatgatt ccgatatcga tcaggttaag cagatcctga
    13381 ccaatattat ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact gtgcgcctga
    13441 acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt ctggagcaac agcggcgatc
    13501 tgcaaaacgt gtactgggat gtgctggagc gtattaaacg tgaatttgat gccgccggta
    13561 tcagcttccc gtacccgcaa atggatgtga actttaagcg ggtgaaagaa gacaaagctg
    13621 cgtaatcaac gctgaggcca gataatactc tctggcctct ctcttattag ttattctgat
    13681 tgccaattaa tattatcaat ttccgctaat aacaatcccg cgatatagtc tctgcatcag
    13741 atacttaatt cggaatatcc aacgtgtttt cttattactt tcaaggtctt gctcttgggg
    13801 cagctatgat tctgccgctc ggcccacaaa atgcttttgt gatgaatcag ggcattcgtc
    13861 gtcagtacca cattatgatt gccttacttt gcgctatcag cgatttggtc ctgatttgcg
    13921 cggggatttt tggtggtagc gcgttattga tgcagtcgcc gtggttgctg gcgctggtca
    13981 cctggggcgg cgtagtcttc ttgctgtggt atggttttgg cgcttttaaa acagcgatga
    14041 gtagtaatat tgagttagct agtgccgaag tcctgaagca aggcagatgg aaaattatcg
    14101 ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta cctggatact tttgttgtac
    14161 tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg ttggtttgcg ctcgggacaa
    14221 ttagcgcctc tttcctgtgg ttctttggtc tggctattct cgcagcctgg ctggcaccgc
    14281 gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt agtgggatgt gtcatgtggt
    14341 ttatagcctt gcagctggcg agagacggta ttgctcatgc acaagccttg ttcagttagg
    14401 cgctatctga tggaaaaata aaacagaggc gctaagcttg cctccagagg tcctgaattt
    14461 tgggcaagtc aattaacacg gagagactaa cgtgaagttc aaagttatcg ccctggcggc
    14521 attaatgggt attagcggga tggcagcgca ggctaacgaa ttgccggatg gaccgcatat
    14581 tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac attgccactc ttgcgattga
    14641 agttaacgtg gccgcgaagg atgccgctac agccaagaaa caggcagatg agcgcgtcgc
    14701 acaatacatt tccttccttg aactcaatca gatcgcgaaa aaagatatca gctcagcgaa
    14761 tttacgcacc cagccagatt atgattatca ggatggtaaa agtatcctga aaggctaccg
    14821 cgctgtgaga acggtggaag tcacgctccg tcagttagac aaactgaatt ccttgctgga
    14881 tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg tcgctgggcg tggcgcagcc
    14941 ggatgcctat aaagacaaag cgcgtaaggc agcgattgat aacgcgattc atcaggcgca
    15001 ggaactggcg aacggctttc atcgtaaact ggggccggta tatagcgtgc gctaccatgt
    15061 ttccaactat cagcccagcc caatggtgcg gatgatgaaa gccgatgccg cgccggtgtc
    15121 tgcccaggaa acttacgagc aggccgctat tcagtttgat gatcaggtcg atgtggtctt
    15181 ccagttagaa cctgtggatc aacaacccgc taaaacacct gcagcacaat aatacttgta
    15241 gcccgacgta ttcgtcgggc taatgccttt acccgatatt gcctcctttt tctgtaattt
    15301 tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg tttcatcaag gataggctga
    15361 ctgaaacggt taagcggaca aggatgcggg gaggggggat ggacattttt atttcgaaaa
    15421 agatgcgcaa tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa
    15481 tccatatgac ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct
    15541 atacgctatt tacccgcaaa gataataata tcagcctcaa taaagccggt caggaactgt
    15601 atcaaaaact gttcccggtt tatcaacgac tttctgcgat tgataatgaa atccataact
    15661 cagggcgtcg ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt
    15721 tcgatcagtt gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt
    15781 tcagcgaaaa cggtgttatc gataatcttt ttgaccgtca gttagatttt attatctccc
    15841 cacagcatgt atctgcacga gttcaggaac tggaaaacct gactatcagt gaattaccac
    15901 cgctgcgatt gggctttctc gtttctcgcc gttatgaaga gcgtcaagag caagagctgc
    15961 ttcaggagct gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga
    16021 ttgatgcgaa tatgcgacct tgtgggatca atcccaccat tatttatcgt ccctacagtt
    16081 ttatggccaa aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg
    16141 cctggcgact ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt
    16201 atatgcagga atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc
    16261 atattgctga agatcgtgac gggacaaacc attaacccag catcgagccg gttgcaatta
    16321 aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat
    16381 tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatag
    16441 tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt tcggtgatga
    16501 tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga
    16561 tcttgccttc tttagcaatc gacggagcca taaagatcga cagatacgcg ttgcgttcaa
    16621 aatcaccgct gccgccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg
    16681 catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga
    16741 tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat
    16801 ccatattgtc gtaaatcttg cgcagggaat cggccgagat tgtcaggctg gaggcgctgg
    16861 ccccgctgat tttgccggtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat
    16921 acatcatgaa cggaggaatt tccgggtttt cccccagacg cgccattacc gcattattga
    16981 tattgcccac gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt
    17041 cctgcaataa gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat
    17101 ccagcatatt accggcgtcg ggcaagttgg tttccacgac ggcgacaatc tttttcggat
    17161 cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc
    17221 gccgcggtgg cgcgccagga atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat
    17281 agtgattaag ttcaatgatc actttcttcg ctcgcagcag ccaggtcggc gcattaccga
    17341 tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa
    17401 catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt
    17461 caacgaaact caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg
    17521 gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg
    17581 acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc
    17641 tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc cggggtaaag ccgctaaatg
    17701 ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact
    17761 gagtttccat ttttactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg
    17821 tttttcgagg aaagcgttca ttccttcctg gtaatcttcg ctgtcataaa ccgcgcgtcg
    17881 catcccctga atacgttcaa attcatcgga gttcatggtg tgcgcttcgc ccagtacacg
    17941 cagctcttct ttgataacag caatggctaa cggcgctttc tcagagatgt ggtgcgccat
    18001 ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc
    18061 caacgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agttctttga caatgtggaa
    18121 gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac
    18181 aggcgtcatt gagaaggttg aggtactggc ggcgataatc agatcggaac tcatgatcat
    18241 ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt tcgggaattt
    18301 ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat cgcgaccgcc
    18361 agacggcagt tcgtgaatat cgtggcctgc ggagaagact ttggatccac tcggcgcgcg
    18421 caaaatgata cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc
    18481 aataaagact ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac
    18541 tttgttgata gtgacaacgt taacatactg ataagacata aaaattcctt taatcaaaat
    18601 attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt
    18661 ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggt
    18721 gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca
    18781 cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga
    18841 tctcatcgat tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt
    18901 cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat
    18961 tggtatggtt atcgccatcg tctttgttga taacgatcag atcagccact tccatcagcc
    19021 cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt
    19081 ccaccatgcg ggcgacttct gtttccgact gcccgacgcc aaccgtttcg acaatcacta
    19141 cgtcataacc cgctgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc
    19201 ccagatgacc ggaggatggt accgggcgaa taaacgccgc ttcggcacgc gccaggtcat
    19261 tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg
    19321 caataaccgc gacctttaat ccctctcgaa tcaacaacat gccaaaggcc tcaagaaagg
    19381 tacttttccc cgcgccgggg gtgccggtaa cgcccagtcg cagggtgtta ccgcagtacg
    19441 gcataatggc atcaagcagc tgcgtactta gtgcctgatg acgcgggtga cggctttcca
    19501 ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg acgtaagcgg cgaatacttt
    19561 ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcaca
    19621 ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag
    19681 gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc gcgtccccat
    19741 tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat
    19801 gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc aggtgtagag
    19861 aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttc
    19921 gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg gcgacgacca
    19981 ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga ggccgatttc
    20041 tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg caccagataa
    20101 cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagcg
    20161 gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc
    20221 aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt
    20281 tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc
    20341 agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg
    20401 cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg
    20461 ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat ttgatcggtc
    20521 agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct
    20581 tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt aggcaaacca
    20641 agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt cgcagccagt
    20701 gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct
    20761 gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc aaatccactg
    20821 accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc
    20881 atgccgatac cgaagaagaa cgacaggcga ggagcgaagt catcaatttt cagtccggca
    20941 gagattgctg ctttgatgta ctcaatccca tcagcgagcg taaatgctac ctgctgcacg
    21001 cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc
    21061 atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg ttttggtggg
    21121 taaatatagg tgttgcggca gaggtactct ttgaggatat cgttctgaat ggtgccggtc
    21181 agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc cagtactggt
    21241 agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac
    21301 aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac gtcgcccgcc
    21361 acgcgcgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc aacggaaaga
    21421 cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa
    21481 aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca
    21541 cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct
    21601 tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca
    21661 gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg cacgttagac
    21721 attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcctacgg tgagcttttt
    21781 acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat
    21841 ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa cgcctgatat
    21901 gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga
    21961 gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga
    22021 gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg gccagctctt
    22081 tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca agttgtacga
    22141 acgcttctga agaattaacg atatggcagg gcacgctgcc tggaggcaga tcgaagtttt
    22201 gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact ggcgctttca
    22261 gtaatgccga acgcgttacg ccgttaggga aatatttttc ggcaaagggt tttgagctga
    22321 cgaacagata gtcgagcgca ccaagtttat cgacaagaca actcggcagc gcctgatgtt
    22381 gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat
    22441 cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc agtgcaggaa
    22501 gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc ggagtcgaac
    22561 cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc agcagtgcca
    22621 gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc
    22681 cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct
    22741 tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc
    22801 tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat
    22861 gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca tttgtattcg
    22921 tggttaaacg cgcagcttaa aagatcaggg atttgcgcta atgcatttcg ctcagcgagt
    22981 tcgcgccctg gttgtgttga atggcgtggc gttattgcct ctgttcgcac cgtggagcgg
    23041 catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg ccattgcgcg
    23101 atattttatg gatgagttaa ccacgcggct tgccaacggg gtctgaatcg ctttttttgt
    23161 atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag gatgaattga
    23221 aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggcaccatt gttggtgtag
    23281 gtacaggttc caccgccgca cactttattg acgcgctcgg tacaatgaaa ggccagattg
    23341 aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg
    23401 tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca gatgaaatca
    23461 acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg
    23521 cttcggttgc agaaaaattt atctgtattg cagacgcttc caagcaggtt gatattctgg
    23581 gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg gcgcgtcagc
    23641 tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat aatggcaacg
    23701 tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa aacgccataa
    23761 atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc
    23821 tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg ggaacctccc
    23881 ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt accaggcaat
    23941 tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat tacctttgcg
    24001 tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta ttttttgata
    24061 tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt gggtaaatgg
    24121 caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc gtgcaccaaa
    24181 aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac aaaggcgcgc
    24241 tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc ctgcgatccc
    24301 gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct attggctgtt
    24361 tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg atcccggtat
    24421 ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc gaactgctgc
    24481 tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg tggaacaaac
    24541 tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc tacggtcata
    24601 ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac ttttatgata
    24661 ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct gacctgctga
    24721 atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg
    24781 gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc tcgcgcggta
    24841 ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg gcgggggcgg
    24901 caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc tctccactgt
    24961 gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag gaagcgcagg
    25021 agaatatcgg cctggaagtt gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc
    25081 tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt cgtctgatgc
    25141 acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc gccgagcagg
    25201 gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat gtagttattg
    25261 atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg
    25321 gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa cgggcaggtc
    25381 actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg cattctactt
    25441 tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt tatttaatgg
    25501 ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc gaagcgaggg
    25561 cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg ttactgataa
    25621 gtttcggagc ttattaacag tcagtctcag gggaggagtc atcctccctt acccttactc
    25681 actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg catcgccctg
    25741 aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata gatccttttt
    25801 cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga aaagatttaa
    25861 aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg cagcatgggg
    25921 ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta tgatgcgact
    25981 tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac agtcagtcac
    26041 aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga atcaacgatg
    26101 tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga cggtgtaacc
    26161 accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa ctgacaatca
    26221 tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt ccgatcataa
    26281 aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag cggtgtgatc
    26341 agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg ctcatggatc
    26401 ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag caaattaccg
    26461 gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca gagttgttct
    26521 attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac cgtatgcgcc
    26581 atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat ttcctgctgt
    26641 tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc ttgtcgggat
    26701 aatgtcagtg ggagttctgg tagttgcgtc atggctggtg ccagataaga agggaatctc
    26761 cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat gtgtcgtcgc
    26821 agttttaagg cttctcggac ggaccgagca tgctcaccaa ccgcggagcg ccacattctt
    26881 gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag agaaattttg
    26941 tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt tggttagttt
    27001 tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc agcatccgaa
    27061 tacgctgttc catacttgcc gcgtagtcac gagtcttcgc tttttcttgc gctaactcat
    27121 agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact ctagtgcgtt
    27181 ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac gcatcccttt
    27241 ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg acgggttgtg
    27301 cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac ccatggtctg
    27361 cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc tggtccacca
    27421 gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat gcttatgtct
    27481 atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca acaagggacg
    27541 ggtctgaccc ctgctgagat gcatggttta atcagtggga tgatatgtgg cggtaacgat
    27601 gacagctcat ggctaccgct acttcacgac ctgacgaacg aaggcatggc tttcggtcat
    27661 gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct gcaggatgac
    27721 ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt cgatcgggct
    27781 gatgcattgg caggttgggt caatcacttc ctgcttggtc ttggcgttac gcaaccgaag
    27841 ctggataaag tgaccggcga aaccggtgaa gctatcgacg atctgcgtaa cattgcgcaa
    27901 ctgggttacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga agagatcatc
    27961 gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc gcaaccgacc
    28021 gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg agagtgttat
    28081 gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg agcaaatgca
    28141 acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg ccgacagcga
    28201 atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg aaccggaagc
    28261 ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt ttaaccgcgt
    28321 tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg ccgcgccaga
    28381 gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac tttatcaact
    28441 acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg ctgatgaaat
    28501 cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca ccgcaccggc
    28561 aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat cgccagaaga
    28621 gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacataccc gggcgatgga
    28681 aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc acgaatttaa
    28741 ccgccacggt gcgcgctatc cgtcctataa caccattgtc ggcagcggtg aaaacggctg
    28801 cattctgcac tacaccgaaa acgagtgtga aatgcgcgac ggcgacctgg tgttgattga
    28861 cgcgggttgc gaatacaaag gttacgctgg cgatattacc cgcaccttcc cggtcaacgg
    28921 caaattcact caggcccagc gtgaaatcta cgacattgtg ctggagtctc tcgaaaccag
    28981 cttgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag tggtgcgcat
    29041 catggttagc ggcctggtaa aactcggcat tctgaaaggt gatgttgatg aactgatcgc
    29101 tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag ggctagatgt
    29161 ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg ggatggtact
    29221 gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac aatatcgcgg
    29281 tatcggcatt cgtattgaag acgacattgt gattaccgaa accggcaacg aaaacctcac
    29341 cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggcagctg cgagaaagca
    29401 atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct ggctatttcc
    29461 cggttaagtc acggggcgct gccggtacat ttgattgaag cgaccgcgcc agagtcacat
    29521 gctcatccgg gctttgatgg acgagctatt gcgctggctg cggggacctg tcagcaactg
    29581 gcgcgcatcg gcgtctggca atctctggcg gattacgcaa ccgccatcac taccgtgcat
    29641 gtcagcgatc gtggtcacgc tggatttgtc accctcgccg cagaagatta ccaactggcg
    29701 gcgctgggac aggttgtcga attgcacaat gtcgggcaac ggctgtttgc attgctgcgt
    29761 aaagcacctg gcgtaacgct gcattgccct gatcgcgtgg ctaacgttgc ccgtactcag
    29821 agtcacgttg aagtgacgct ggagagtggc gagacgctga cgggccgcgt gctggtagca
    29881 gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgactggca gcaggagcct
    29941 tacgaacaac tggccgtgat tgccaacgtt gcgacttccg ttgcgcacga aggccgcgct
    30001 tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga cggacgctgt
    30061 tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg gagcgacgag
    30121 aagttttgcc gtgaactcca gtcggccttt ggctggcgac ttggaaaaat tacccacgcc
    30181 ggtaaacgca gtgcttatcc gctggcgtta acccgcgccg ccagagctat tacccatcgt
    30241 accgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca agggtttaac
    30301 ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca tgagcgcgga
    30361 gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca gagtgatcgc
    30421 gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg ttgggcaccg
    30481 ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc ggcacgcgat
    30541 gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta aggaataaac
    30601 aatgcaaagt gttgatgtag ccattgttgg tggcggcatg gtggggctgg cggttgcctg
    30661 cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac cggaacctct
    30721 ggcggcggat gcaccaccac aactgcgcgt ttcggctatc aatgccgcca gcgaaaaatt
    30781 actcacccgt cttggcgtct ggcaggacat tctctctcgt agggccagtt gttatcacgg
    30841 tatggaagtg tgggacaaag acagttttgg tcacatttcg tttgacgatc aaagcatggg
    30901 ctatagccat cttgggcata tcgttgaaaa ctcagtgatt cactacgcgc tgtggaacaa
    30961 agcgcagcag tcgtcagata tcactctgtt ggcccccgca gaattacagc aggtcgcctg
    31021 gggagaaaat gaaactttcc tgacgctgaa agatggcagc atgttaacgg cgcgtctggt
    31081 gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc cgctgacttt
    31141 ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac cgcatgatgc
    31201 ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc ttagcgatcc
    31261 gcatctttgc tcaattgtct ggtcactgtc gccagaggaa gcgcagcgga tgcagcaggc
    31321 aagtgaagat gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc tgggcttatg
    31381 caaggttgag agcgcgcgtc aggtgttccc actgacgggg cgttatgcgc gccagtttgc
    31441 cgcgcaccgt ctggtgctgg tgggcgacgc cgcgcatacc attcacccgc tggcggggca
    31501 gggagtaaat ctcggcttta tggatgctgc agagctgatt gctgaactga aacggttgca
    31561 tcgtcagggg aaagacatcg ggcagtacat ttacctgcgc cggtatgagc gtagccgcaa
    31621 gcacagtgcg gcgctgatgc tggctggtat gcagggattc cgcgatctgt tttccggtac
    31681 caatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca cgcttcctgg
    31741 cgttaaacca caacttatcc gccaggcaat gggattaaac gatttgcctg aatggctgcg
    31801 ttaaaaattt ctcttctgct gtttatttga tacccatcac actttcatct cccggttttt
    31861 tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt cgcattatat
    31921 tttctaatgt cacgaatttt tgatttagtg ttttttgaca ttttttcagc tcttaatatt
    31981 gccttattca aattaacttt ctcatcacat catctttata taaaaacttg tgtatttttt
    32041 ggttttttat tctgttgcga ttttggcgtt ttttaaccat aagctaatgt gatgatcaat
    32101 tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg
    32161 ttggggaccg ggagtggctc cgatgctggg tttcgtggtg ataatttcac catgaaaaag
    32221 ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt tgtacgaaca
    32281 acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc cgctgcatta
    32341 cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt ttgatgtgtc
    32401 acatatgacc atcgtcgatc ttcgcggcag ccgcacccgg gagtttctgc gttatctgct
    32461 ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa
    32521 tgcctctggc ggtgtgatag atgacctcat cgtctactac tttactgaag atttcttccg
    32581 cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc aacacgctga
    32641 acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgatcgccg tacaagggcc
    32701 gaatgcgcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg cggtggaagg
    32761 gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt attgccacca ctggttatac
    32821 cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc
    32881 gctggtggaa gcgggcgtta agccatgcgg tctgggcgcg cgtgacacgc tgcgtctgga
    32941 agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt tagccgccaa
    33001 catgggctgg actatcgcct gggaaccggc agatcgtgac ttcatcggtc gtgaagccct
    33061 ggaagtgcag cgtgagcatg gcacagaaaa actggttggt ctggtgatga ccgaaaaagg
    33121 cgtgctgcgt aatgaactgc cggtacgttt taccgatgcg cagggcaacc agcatgaagg
    33181 cattatcacc agcgggactt tctccccgac gctgggttac agcattgcgc tggcacgcgt
    33241 gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa
    33301 agtgacgaaa cctgtttttg tgcgtaacgg taaagccgtc gcgtgattta cttttttgga
    33361 gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca cgaatggctg
    33421 cgtaaagaag ccgacggcac ttacaccgtt ggtataaccg aacatgccca ggagctgtta
    33481 ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac
    33541 tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt atgcgccagt aagcggtgaa
    33601 atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag cgaaccgtat
    33661 gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga atcactgctg
    33721 gatgcgaccg catacgaagc attgttagaa gacgagtaac ggctttattc ctcttctgcg
    33781 ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt aaggagagag
    33841 gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt taagccagct
    33901 tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc aacagcaaga
    33961 aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga ttgtgccgaa
    34021 agatattcag cttgcgaccc cgccgcaggt tggcgcaccg gcgaccgaat acgccgcact
    34081 ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca tcggcatggg
    34141 ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa atccgggctg
    34201 gtataccgcg tacactccgt atcaacctga agtctcccag ggccgccttg aagcactgct
    34261 caacttccag caggtaacgc tggatttgac tggactggat atggcctctg cttctcttct
    34321 ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca gcaaactgaa
    34381 aaatgccaac cgcttcttcg tggcttccga tgtgcatccg caaacgctgg atgtggtccg
    34441 tactcgtgcc gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa aagtgctcga
    34501 ccatcaggac gtcttcggcg tgctgttaca gcaggttggc acaaccggtg aaatccacga
    34561 ctacaccgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagtg tcgccgccga
    34621 tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata ttgtttttgg
    34681 ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg ccttctttgc
    34741 ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat cgaaagatgc
    34801 agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata ttcgccgtga
    34861 gaaagcgaac tccaacattt gtacttccca ggtgctgctg gcaaacatcg ccagcctgta
    34921 tgccgtttat cacggcccga ttggcctgaa acgtatcgct aaccgcattc accgtctgac
    34981 cgatatcctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg cgcactattt
    35041 cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg ccgaagcggc
    35101 tgaaatcaac ctgcgtagcg atattctgaa cgcggttggg atcacccttg atgaaacaac
    35161 cacgcgtgaa aacgtaatgc agcttttcag cgtactactg ggcgataacc acggcctgga
    35221 catcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc ctgcgatgct
    35281 gcgcgacgac gaaatcctca cccatccggt gtttaatcgc taccacagcg aaaccgaaat
    35341 gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg cgatgatccc
    35401 gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa tcacttggcc
    35461 ggaatttgcc gaactgcacc cgttttgtcc gccggaacag gctgaaggtt atcagcagat
    35521 gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg tttgtatgca
    35581 gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc attatcatga
    35641 aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc acggaactaa
    35701 ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg ataaaaacgg
    35761 caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata acctctcctg
    35821 tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc gtgaagtgtg
    35881 tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca tgaacgccca
    35941 ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca acctgcataa
    36001 aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg gtgtgaaagc
    36061 gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca tgttaacccg
    36121 tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa tcagctggat
    36181 gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg caatcctcaa
    36241 cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata ccggtcgcga
    36301 cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag aaaccggcat
    36361 cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc cgaccatgtc
    36421 gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca aagtggaact
    36481 ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa atcgaccagg ttaaagccgg
    36541 tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga acgaactggt
    36601 cgccgagtgg gcgcatccgt acagccgtga ggttgcagta ttcccggcag gtgtggcaga
    36661 caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta acctgttctg
    36721 ctcctgcgta ccgattagcg aataccagta attcactgat tcgactattt tctaaaggcg
    36781 cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt cccctcgccc
    36841 ctttggggag agggtgaggg gaacaggccg gcactggcgc gaatatttac cctcaccccg
    36901 gccctctccc tgaaagggcg agggggaaaa gcgtgccaac attgaagatt gagccagttt
    36961 gttagcaatc tcaaagatac gtcaacgaat gaatttttct cggaaaaaca aatggctata
    37021 gcacttgtga ccggtggcag tcgcggcatc gggcgggcaa ctgcattact gttggcgcaa
    37081 gaagggtata cggtggcggt taattatcag caaaacctcc acgcggcgca ggaagtgatg
    37141 aacttaataa cgcaagccgg tggcaaagca ttcgtgctcc aggcggatat cagcgacgaa
    37201 aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg atgaaccgct agcagcgctg
    37261 gtcaataacg ccgggatctt gtttacccag tgcaccgttg aaaaccttac cgcagagcga
    37321 atcaaccgag tactttccac caacgtgacg ggatattttc tctgctgccg cgaggcggta
    37381 aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta tcgtcaatgt ctcttcggtg
    37441 gcctcacggt tgggttcgcc aggggaatat gttgattacg cggcatcgaa aggggcgatt
    37501 gatacgttaa ccaccggact atcgctggaa gtcgccgcgc aggggatccg cgttaactgc
    37561 gtgcggccag ggtttattta taccgaaatg cacgccagcg gcggcgagcc tggacgcgtc
    37621 gatcgcgtta agtcgaacat ccccatgcag cgtggtggac aggcagaaga ggtcgcgcag
    37681 gccattgtct ggctactaag tgataaagcc tcttacgtca cgggaagttt tatcgatttg
    37741 gcgggcggga aataaaacag ggaagttgtc tgaccggatg caacaagtat tgcatccggt
    37801 acttcatcga cttaaagctt ctcgccgttg ctggcaatca cctcttggta ccagttaaag
    37861 ctcttcttac gtgaacgcga catatcgcca gcaccgtcgt catgtttatt cacatagata
    37921 aagccgtagc gtttgctgta ctgcccggtg gtgaacgata cgcagtcgat gcagccccac
    37981 ggtgtgtagc ccatcagatc cacgccatcg taagtcaccg cttttttcat ctcttcgata
    38041 tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc tgccatcttc ttccactttg
    38101 tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc tctgataacg ctcatacagt
    38161 tcgcaaaggg catagcgcag acctactgga tcaatctgcc agccccagtc agacgcttta
    38221 acatacgggt tcggtacgct gccttcgaaa ccagagatcg catcgccggt gccgccttcg
    38281 gccttcactg cgttggtcat gtaatagctg aaaccaagat aatcgcaggt gccttcacgc
    38341 agtacatcga gatcgccgtc ttccattttg atgttaaatc cgcgacgctc ccactcgttc
    38401 aacacatagg acgggtaata gccgcgtagc tgcacatcgg taaagacgta gcgttcgcgc
    38461 atcgactcct gagcgaacat cacatcgtcc gggttacagc agtagggata gagcggcacc
    38521 atcgccagca tacagccgac tttcatctcc gggtgaatgc gacgcgcagc tttcaccgcc
    38581 agggcgctgg cgacaaactg gtgatgcagc acctgataca tcgtctcttc cgggttctca
    38641 tgctcggtat aaaccacgcc ggagcagcag taaccgaaca gcggtgcacg ccagttacgc
    38701 tggttgttaa tttcgttgaa ggtcatccag tatttgactt tatgcttata gcgttcaaac
    38761 accacttccg cgaaacggac aaagaaatca accactttac ggttggtcca gctaccgtat
    38821 tgctgcacca gatgcagcgg catttcaaag tgggagaggg tgatcaccgg ttcgatgttg
    38881 tatttcagca gttcatcgaa catatcatcg tagaacttca gcccttcttc gtttggctga
    38941 gcttcatcac cttttggaaa aatacgcgtc caggcgatgg aagtccgaaa acatttgaag
    39001 cccatttcgg caaatagctt gatgtcttcc ttgtagtgac cataaaaatc aacggcttca
    39061 tggtttggat agtatttgcc tggcaagact tctttggtga tttcgcgcgg cacgccgtgt
    39121 gcgccaccgg tcagaacgtc acaaatgctc ggcccttttc cgcctttgtt ccagccgcct
    39181 tcgacctgat gagcggcaac tgcgccgccc cataagaaat ctttcggtaa ggtgagtttt
    39241 ttcactatca taggctccag atgagtcaat tcttacagga aatagtacca tgcagccaaa
    39301 cgacatcact ttttttcaac gtttccagga tgacattctg gctgggcgta aaaccatcac
    39361 catccgcgac gagtctgaat cgcacttcaa aacgggtgat gtgcttcgtg tcggacgttt
    39421 tgaagatgac ggttattttt gcacgattga agtcaccgca acctcaaccg taacgctgga
    39481 tacgctgact gaaaaacatg cagagcagga aaatatgacc ctgactgaac tgataaaggt
    39541 cattgccgac atctatcccg gtcagacaca gttttatgtg attgaattta aatgtcttta
    39601 aattgaactc gcgcctgtta gttgaaattt cgcatttaat ttcatgattt ttaggagatt
    39661 agtctcaaat cagaatttat tttagctaac aggtgttcac tggaactatt ctcagttacg
    39721 ctggagaggt atacatgtgc gtgtacgttt ctccggagta agttatggtt cagaagcccc
    39781 tcattaagca gggatattcg ctggcagagg aaattgccaa cagcgtcagt cacggcattg
    39841 ggttggtgtt tggtatcgtt gggctggtgt tgctactggt tcaggcggtg gatctcaatg
    39901 ccagcgccac tgcgataacc agttacagcc tctatggcgg cagtatgatc ctgctgttcc
    39961 tcgcttcgac gctctatcac gccattcctc atcaacgggc aaaaatgtgg ctgaagaaat
    40021 ttgaccattg cgctatttac ctgttgattg ccggaaccta cacgccgttt ttgctggtgg
    40081 ggctggattc tccgttagcg cgcgggttga tgattgttat ctggagcctg gcattgctgg
    40141 gtattctgtt taaactgacc atcgcgcacc gattcaaaat tttatctctg gtgacctatc
    40201 tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat ggcagttaag ctcgcggcgg
    40261 gcagcgttac cttactggcg gtaggcggcg tggtttattc gctcggggtg attttctacg
    40321 tctgcaaacg cattccatac aaccatgcca tctggcacgg cttcgtgctc ggcggtagtg
    40381 tgtgccactt tctggcgatc tatttgtata ttgggcaggc gtaatgggta tgcctttgta
    40441 ggcctgataa gacgcgccag cgtcgcatca ggcatcggag cacaactgcc ggattcggcg
    40501 tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg ataaaggcat caaatcccag
    40561 acatgataag acgcgtcagc gtcgcatcag gcgataaatg cctgatgcga taaagagatt
    40621 tactcttcga gcgaatacgg cagcggctcg atatgcaatg tattcgcatc gtcgcgtacg
    40681 cggaagatgc tatccggttc catatcgtta ttcatgacga cctgtaccac gacctgacca
    40741 tcttccagtt ttaccgcagc cagcaccgta ccggtacgac gccagttctc gcccattttc
    40801 agctctaagt cttcaccagc ttccggcagt cggctggcgc tacctgccag caaccagagc
    40861 gcacgtttat tggcaccacg gaattttgct cgcgccacca tctcttgtcc ggtataacag
    40921 cctttcttaa agctgatacc gcccagcgcc tggagattgg tcgcctgtgg gataaactgc
    40981 ccgctgttgg cggcatcaat caccgggaaa cccgcttcaa tgtttaatgc cagccactgt
    41041 tggctattgt tcagttccgc ttcaccgcgc agtttatcgg tcagcatatt agcagtagct
    41101 tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa accatagcaa agtggtcgcg
    41161 ccttctttga ctacctgttt ttctttcgaa ggcagttcgc taaagagatt tgccagcgcg
    41221 gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc
    41281 actttagaga ataccgcata ttttttcagt tcagtcagct gcggttcacg cacgctgcgc
    41341 cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac gtaaattgct ccacatttta
    41401 cctttggcgt cgcaatgggc ggcgagcagg tgctgatttt ctgtcatctg gctgacatct
    41461 gctgtcacct gaccctgcat atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc
    41521 cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag aagccgtagg ctgacgggga
    41581 ggaaaaggtg taaaagccat aatagagtcc tgagttgctt aacggcgtat taatgactaa
    41641 tggtaaaaga gctatgaggc aatgcaagtg ctttcactac gcgatctgtg ccttctgcga
    41701 ggcgacttcc aaaaaaatga agccattgaa ttgttgtgaa ttaatttcgc gatcgcgatg
    41761 gtgtttactg atattcccgt aaaagaaggc gaaaaacacg ttacaatagc ggggtactgg
    41821 attttcgtga gacaggaaga agatggacat taacaacaaa gcccgcattc attgggcatg
    41881 ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg tttttcgaac atgagtacga
    41941 cagcttaagc gatgacgaaa aacgcatctt tattcgtctg ctggaatgtg acgatctgga
    42001 cctgtttaac tggctgatga atcacggtaa accggccgat gcagaactgg aaatgatggt
    42061 ccgactcatc cagacacgga accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct
    42121 cctggcgcgc acagtggctt tccttgctga ttcatgggct ggttgccgct gttattttac
    42181 tcatgccctg gccactcagt tacaccccgt tatggatggt gttactttcg ctggtggtgt
    42241 ttgattgcgt tcgcagccag cggcgtatta atgctcgcca gggggaaatt cgcttgttga
    42301 tggacgggcg tttgcgttgg caagggcagg agtggagcat cgtcaaagca ccgtggatga
    42361 ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg taaacggcaa catttatggc
    42421 tggcagccga cagcatggac gaagctgaat ggcgggattt acggcggatt ttgttgcaac
    42481 aagagacgca aagataaaga tgacggcaaa gcatatgcgc taagccgtca gtgagatcag
    42541 gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc agctctgaat acgctcgtcg
    42601 ctaaggtcat actggttagt ttcatccagc gccagaccca cgaacagttg cccgtcagca
    42661 atcaccggtt tcgggctggt aaattcatat ccttccgttg gccagtagcc gacgaacttc
    42721 acgcctttgg tcgagagttt gtcatgcagc ataccgagcg catcgaggaa ccactcgccg
    42781 tatcccagtt gatcgccaag cccatacagc gcaacaattt taccttcaag gttcaggtcg
    42841 tcgagctgat cccagacggc ttcccagtct tcctggattt caccaaaatc ccaggtcggg
    42901 atacccagaa tgagcacatc gtactgctcc attaatttcg gggagtcgtc cttgaggtta
    42961 tgtaaggtca ccagttctgg gccgataata tcgcggattt tttctgccgc catttcggtg
    43021 taacaggtgc tggaaccgta aaaaagaccc atattcataa cgtaagcttc tcgattctgc
    43081 tgtggctttt gcagctagtg tatcagaatc gctttagtca cggcacaata aggcataatg
    43141 gatcaaatga gtgaccataa ggggcgcaag tgaaacagga tctggcacgc atcgagcagt
    43201 ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc
    43261 gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc
    43321 aaagtgacga tttgcaggca ttactggcag aacggctgga gggcgggtat aaagccacca
    43381 gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt
    43441 ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa attgccccag cgtttgccaa
    43501 aagatttaag cgaagcgcag gtcgaacgtt tattacaggc accattaatt gatcagccac
    43561 tggagctacg cgataaagcc atgcttgaag tgttgtatgc taccgggctg cgtgtctctg
    43621 aactggtcgg actgacaatg agtgatatca gcctgcgtca gggcgtagtg cgggtcattg
    43681 gtaaaggcaa caaagagcgc ctggtaccgt taggtgaaga ggcggtttac tggctggaaa
    43741 cctatctgga acatgggcgt ccgtggctgc tgaatggtgt gtcaattgac gtgttgtttc
    43801 ccagccagcg tgcgcagcag atgacgcgac agaccttctg gcaccgcatt aaacattatg
    43861 ctgtgctggc gggtatcgac agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg
    43921 ccactcattt attaaatcat ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca
    43981 gcgatctttc caccacgcaa atttatacgc atgtcgctac cgagcgtctg cggcaacttc
    44041 atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag
    44101 gttttatgtt gtttactttg ttagcggcgt tttcaggttt tgctcaggct gatgacgcgg
    44161 caattcaaca aacgttagcc aaaatgggca tcaaaagcag cgatattcag cccgcgcccg
    44221 tagctggcat gaagacagtt ctgactaaca gcggcgtgtt gtacatcacc gatgatggta
    44281 aacatatcat tcaggggcca atgtatgacg ttagcggcac ggctccggtc aatgtcacca
    44341 ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcgc
    44401 cgcaggaaaa acacgtcatc accgtgttta ctgatattac ctgtggttac tgccacaaac
    44461 tgcatgagca aatggcagac tataacgcgc tggggatcac cgtgcgttat cttgctttcc
    44521 cgcgccaggg gctggacagc gatgcagaga aagaaatgaa agctatctgg tgtgcgaaag
    44581 ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg
    44641 acgtggatat tgccgaccat tacgcacttg gcgtccagct tggcgttagc ggtactccgg
    44701 cagttgtgct gagcaatggc acacttgttc cgggttacca gccgccgaaa gagatgaaag
    44761 aattcctcga cgaacaccaa aaaatgacca gcggtaaata attcgcgtga aacaacagat
    44821 acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg cccgctgaat tgcctccctt
    44881 gctgcgccgt ttatacgcca gccggggcgt gcgcagtgcg caagaactgg aacgcagtgt
    44941 taaaggtatg ttgccctggc agcaactgag cggcgtcgaa aaggccgttg agatccttta
    45001 caacgctttt cgcgaaggaa cgcggattat tgtggtcggt gatttcgacg ccgacggcgc
    45061 gaccagcacg gctctaagcg tgctggcgat gcgctcgctt ggttgcagca atatcgacta
    45121 cctggtacca aaccgtttcg aagacggtta cggcttaagc ccggaagtgg tcgatcaggc
    45181 ccatgcccgt ggcgcgcagt taattgtcac ggtggataac ggtatttcct cccatgcggg
    45241 ggttgtacac gctcgctcgt tgggcattcc ggttattgtt accgatcacc atttgccagg
    45301 cgaaacatta cccgcggcgg aagcgatcat taaccctaac ttgcgcgact gtaatttccc
    45361 gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg atgctggcgc tgcgcacctt
    45421 tttgcgcgat cagggctggt ttgatgagcg tggcatcgca attcctaacc tggcagaact
    45481 gctggatctg gtcgcgctgg gaacagtggc ggacgtcgtg ccgctggacg ctaataatcg
    45541 cattctgacc tggcagggga tgagtcgcat ccgtgccgga aagtgccgtc cggggattaa
    45601 agcgctgctg gaagtggcaa atcgcgatgc gcaaaaactc gccgccagcg atttaggttt
    45661 tgcgctgggg ccacgtctca atgctgccgg gcgactggac gatatgtccg tcggtgtggc
    45721 gctgttgctg tgcgacaaca tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct
    45781 aaaccagacg cgaaaagaga tcgaacaagg aatgcaagtt gaagccctga ccctgtgcga
    45841 gaaactggag cgaagtcgcg acacgctacc cggcgggctg gcaatgtatc accccgaatg
    45901 gcatcagggc gttgtcggta ttctggcttc gcgcatcaaa gagcgttttc accgtccggt
    45961 tatcgccttt gcgccagcag gtgatggtac gctgaaaggt tcaggtcgct ccattcaggg
    46021 gctgcatatg cgtgatgcac tggagcgatt agacacactc taccctggca tgatactgaa
    46081 gtttggcggt catgcgatgg cggcgggttt gtcgctggaa gaggataaat tcgaactctt
    46141 tcaacaacgg tttggcgaac tggttacaga gtggctggac ccttcgctat tgcaaggcga
    46201 agtggtgtca gacggtccgt taagcccggc cgaaatgacc atggaagtgg cgcagttgct
    46261 gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg ctgtttgacg gtcatttccg
    46321 tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag gtgatggtcg aaccggtcgg
    46381 cggcggtcca ctgctggatg gtattgcttt taatgtcgat accgccctct ggccggataa
    46441 cggcgtgcgt gaagtgcaac tggcttataa gctcgatatc aacgagtttc gcggcaaccg
    46501 cagcctgcaa attatcatcg acaatatctg gccaatttag cgtcatcttc tctataaaaa
    46561 agagcgtgga ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag
    46621 aaatcagacc atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg
    46681 ctccgacgtt cttagggggt atctttgact acgacgccaa gaaagagcgt ctggaagaag
    46741 taaacgccga gctggaacag ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg
    46801 gtaaagagcg ttcctccctc gaagccgttg tcgacaccct cgaccaaatg aaacaggggc
    46861 tggaagatgt ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta
    46921 acgaagccgt tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc
    46981 gtatgttctc tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg
    47041 gcggtacgga agcacaggac tgggcgagca tgcttgagcg tatgtatctg cgctgggctg
    47101 aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta
    47161 aatccgtgac gatcaaaatc tccggcgatt acgcttacgg ctggctgcgt acagaaactg
    47221 gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg cggtcgtcgc cacacatcgt
    47281 tcagctctgc gtttgtttac ccggaagttg atgatgatat tgatatcgaa atcaacccgg
    47341 cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta
    47401 ccgaatctgc ggtgcgtatt acccacatcc caaccgggat cgtgacccaa tgccagaacg
    47461 accgttccca gcacaagaac aaagaccagg ccatgaagca gatgaaagcg aagctttatg
    47521 aactggagat gcagaagaaa aatgccgaga aacaggcgat ggaagataac aaatccgaca
    47581 tcggctgggg cagccagatt cgttcttatg tccttgatga ctcccgcatt aaagatctgc
    47641 gtaccggggt agaaacccgc aacacgcagg ccgtgctgga cggcagcctg gatcaattta
    47701 tcgaagcaag tttgaaagca gggttatgag gaaccaacat gtctgaacaa cacgcacagg
    47761 gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga
    47821 acctgcgcga gcagggggtt gccttcccga acgatttccg tcgcgatcat acctctgacc
    47881 aattgcacgc agaattcgac ggtaaagaga acgaagaact ggaagcgctg aacatcgaag
    47941 tcgccgttgc tggccgcatg atgacccgcc gtattatggg taaagcgtct ttcgttaccc
    48001 tgcaggatgt gggtggccgc attcagctgt acgttgcccg tgacgatctg ccggaaggcg
    48061 tttacaacga gcagttcaaa aaatgggacc tcggcgacat cctcggcgcg aaaggtaaac
    48121 tgttcaaaac caaaaccggc gaactgtcta tccactgcac cgagctgcgt ctgctgacca
    48181 aagcactgcg tccgctgccg gataaattcc acggcttgca ggatcaggaa gcgcgctatc
    48241 gtcagcgtta tttggatctc atctctaacg atgaatcccg taacaccttt aaagtacgct
    48301 cgcagatcct ctctggtatt cgccagttca tggtgaaccg cggctttatg gaagttgaaa
    48361 cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg tccgtttatc acccaccata
    48421 acgcgctgga tctcgacatg tacctgcgta tcgcgccgga actgtacctc aagcgtctgg
    48481 tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt
    48541 ccgtacgtca taacccagag ttcaccatga tggaactcta catggcttac gcagattaca
    48601 aagatctgat cgagctgacc gaatcgctgt tccgtactct ggcacaggat attctcggta
    48661 agacggaagt gacctacggc gacgtgacgc tggacttcgg taaaccgttc gaaaaactga
    48721 ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga catggcggat ctggataact
    48781 tcgactctgc gaaagcgatt gctgaatcta tcggtatcca cgttgagaag agctggggtc
    48841 tgggccgtat cgttaccgag atcttcgaag aagtggcaga agcacatctg attcagccga
    48901 ccttcattac tgaatatccg gcagaagttt ctccgctggc gcgtcgtaac gacattaacc
    48961 cggaaatcac agaccgcttt gagttcttca tcggtggtcg tgaaatcggt aacggcttta
    49021 gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct ggatcaggtt gccgcgaaag
    49081 acgcaggtga cgacgaagcg atgttctacg atgaagatta cgttaccgca ctggaacatg
    49141 gcttaccgcc gacagcaggt ctggggattg gcatcgaccg tatggtaatg ctgttcacca
    49201 acagccatac catccgcgac gttattctgt tcccggcgat gcgtccggta aaataagcat
    49261 tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt aattgtgctg cgcgaaagca
    49321 gacaacaatt ttcttgcttc actattggct gcctgcatca ccatccacgg actgaacgcc
    49381 cacggtgtgg catcaatacc gtgtaataca tctgctaaat cacaccattg ataatccatc
    49441 acttcatcat cgttgatctg taacgcactg gtggtgcgtg cggcaaatac cggacacact
    49501 tcattttcca caatgccatt cggatcggtg gcgcgatagc gaaagtcagg atagatagat
    49561 tcaggaggcg taatttccac gccaagccca taacggcaac ggcggatcac cgcgtcttcg
    49621 ttgctttctc ccagttgtgg gtgcccacaa accgagttag tccacacgcc aggccacgct
    49681 tttttgctca gtgcgcggcg ggtaactaat aattgtcctt tggcattaaa cagccaactg
    49741 gagaacgcgc gatgtaagcg ggtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc
    49801 gtgggaactc cctgtgcatt caataaaatg acgtgttccg tttgcataat ttctcacatg
    49861 taattctgat cactcatcat tatagataat tgaagagtga aatgattaac caagtgagcg
    49921 tttatcgaca accgcccgtt ttgagcggat gccgacaggt aaaaaccatt taaaaaagta
    49981 gcatcatata tttgaagcga ctttcaatgg ccattgcaac acagtcagaa atgacaggaa
    50041 tagagagagt aaaaagcaac aacagactga atatagcgcg cattaaaaaa ttcatattat
    50101 tctccatgct catttttctt cagcttgcgc atcagaagcc acatacgtac tgtcctgact
    50161 tttaaatctt tatcagagac ggattccttc accaggtcag catgatgctg atagccatta
    50221 aagaagacat tcagcacaac ggcgctaagg gttgcgagca taatgccgct atgcagcaac
    50281 ggttgcagta cggccggtaa cttagaaaag aaatcgtgag agagcgtcgg agtcatgcca
    50341 acgccgagac tgatcgccac aatatagagg ttgtaacggt tggtggtgta gttacagcgc
    50401 gacagaattc gaatccctgt cgccagtacc atgccgaaca tcaccagccc agcgccgccc
    50461 agcacaaatt gcggaatgga ggctaccagc accgccattt ttggcaccat gccaaacagg
    50521 attaatataa ttcccgaaga aatacacacc cagcggctat gaacgcgcgt cacgctaacc
    50581 aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt taaacgtgcc gcctatcatt
    50641 gtccctacgc catcgacacg cagcccgcga ataatatcgt gtgaagagag tttacgaccg
    50701 actatttcac ccagtgccag gaacatcccc atcgactcga taaacacgat gattaacacg
    50761 gcagtcatgg tcagaatgga aacgggatcg aaaatcggca taccaaacga catcggggtg
    50821 acaatcgcaa accacgaggc atcatgtagc ccggataaat tgacttcatt catcatccac
    50881 gaaagtaaaa agccaaatac aatccccagt aatacggcga cgttggacat aaatcctttc
    50941 gcatagcgag taatgagcaa gataaaaatt aagacggcaa aggagatacc taaataaacg
    51001 ggattaccat attgcggatt ccctttacct ccggcggccc agtcaatacc cacctgaatg
    51061 atgctaagcc caatagaagt aataaccaca ccggtaacca gtggcgggaa taaaggcatc
    51121 aagcgaccaa taagtggcgc taataatgtg gtgataaaac ccgcggctat agtggcacca
    51181 aatatcccca gcaggccgat atccgggttc atcccaatgg ctatcattgg tgttacagca
    51241 gcaaaggtca ccgacataat caccggcagg tggatcccca taaagcggcc gataccgata
    51301 cattgcaata atgtgacgat cccgcagcaa aagagatccg agctaatgag catcgcaata
    51361 gcttcttttg agaggcccag tcggtcacca atcataagag gaacagcgac tgcacctgcg
    51421 tacatgacaa ggacgtgttg cagaccgagt atgatcagct ttcctggtga taatatgcgg
    51481 tcaacctcat cagttgggcg gtcttgccca gaagatgagg gaagttggga atctatggcg
    51541 ctcatcagtg tttctccttt cgcccgccag agtaaaaagc aaccgtttac gttaatgaaa
    51601 ctgtctaaac acatgctcgt ggagtgccag tttttgcgag gaaggggagt tagagacagg
    51661 aattgcggag gcaatcacat aatcgtggtt aaaccacgaa aatgagaaga gaacctgaca
    51721 atgaaattat ctgtaatttt ctcatattga tatttcatta atcattctgg catgtcgcac
    51781 tctcgcattt aatcgttttt atctggatag cgctcttttg gtcggcaatg ttgaaataat
    51841 gaaagcggca tagattgagg ggcaataatt tgaactcagt cgcaaaatta taattaaaac
    51901 ggtcatgcta cctaatatat gcccgtttat tgcatcgcga aatttgagaa gacataactc
    51961 attaaatgaa agggatgcaa atgaataagt ttatcgctgc tgaagctgcg gaatgtatag
    52021 gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa tcaagaaaac tggccgctga
    52081 gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa aggccaggct gcgaatccgg
    52141 tggcctgcca tcactgcaac aatgcccctt gcgttacggc ttgtccggtt aatgctctga
    52201 ctttccagtc cgatagcgta caactggacg agcaaaaatg tattggctgt aaaagatgcg
    52261 caatcgcttg cccctttggc gtcgttgaga tggtcgatac gattgcgcag aaatgcgacc
    52321 tttgtaacca gcgcagttcc ggcacgcaag cctgtattga agtctgccca acacaggcgt
    52381 tacgactgat ggacgataaa gggttacagt ggataaaggt ggctcgccag cgcaaaacgg
    52441 cagcaggaaa agcgtcatca gatgctcagc catctcgcag tgcagcgttg ctccccgtta
    52501 actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg gaaaacccac tttggcgaaa
    52561 tatattgcgg gctggatcca ctgcaagcga cttatgagag tgaccgctgt gtttattgtg
    52621 ccgaaaaagc taactgcaac tggcattgcc cgctgcataa cgctattccg gattacatcc
    52681 gtctggtaca ggaaggaaag attattgaag cggcagaact ttgccaccag accagttcct
    52741 taccagaaat ttgcggcagg gtatgtccac aggaccgtct ttgtgaaggc gcatgtactt
    52801 tgaaagatca ctcgggcgca gtctctatcg gtaatctgga acgctacatc accgataccg
    52861 cgctggcgat gggctggcgt cccgatgtca gcaaagttgt tccccgtagc gaaaaagtgg
    52921 cggtgattgg cgctgggcca gcagggttag ggtgtgctga tattctggcg cgcgcgggag
    52981 ttcaggttga tgtctttgat cgccatccag aaattggcgg tatgctgact tttggcattc
    53041 ctcctttcaa actcgataaa acggtattaa gccagcggcg agagatattc accgcaatgg
    53101 gaatcgactt tcatcttaac tgtgaaattg gccgcgatat tacctttagc gatttaactt
    53161 ctgaatatga tgcagttttc atcggcgtgg ggacttacgg gatgatgcga gcagatctgc
    53221 cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt cctgactgcc catacccgcc
    53281 agctcatggg attgccggag tctgaagagt atccgctgac ggacgtggaa ggtaagcgag
    53341 tcgtggtatt gggcggtggc gatacgacaa tggattgttt gcggacttcc atccgcctca
    53401 atgccgccag cgtgacctgc gcgtatcgtc gtgatgaagt cagtatgccg ggctcgcgca
    53461 aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca gttcaatgtt caaccgcaat
    53521 atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg cctgattcgt accgccatgg
    53581 gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt agcgggttca gagtttgaat
    53641 tgcccgccga tgttctgatt atggcctttg gtttccaggc gcatgccatg ccgtggttgc
    53701 agggcagcgg aattaaactc gataaatggg gcctgattca aaccggtgac gtcgggtatt
    53761 tacctaccca gacgcatctg aaaaaagtct ttgctggtgg tgatgcagtt catggcgcgg
    53821 atctggttgt cactgcaatg gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt
    53881 ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt aatccggctg actgtattgg
    53941 ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct tcagaacagg agttgaatgc
    54001 cgatgtcttt ttaccccggc tgaaggtaca gcgactggac agcatcagtg cgccggtgat
    54061 gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc cccgtggggg cgctgacgat
    54121 gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt ggctgtcaga gttgcgtcag
    54181 tgcgtgcccg tttggaatga tcaccattca gtcgttgccg ggggataccc gacaacaaat
    54241 tgtgaaatgc gatctctgtg aacagcggga agaggggcca gcctgtgttg aatcttgccc
    54301 gacacaggcg ctgcagttgc ttactgaaag agaactcagg cgagtccgcc agcagcgtat
    54361 tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg cgctgaaagg cgcggagaat
    54421 ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt gcggcagtaa tgatcaccgt
    54481 accaatattc aacttttgcc attcgcgagc aaagacacgg cctacgacca gtgtcacaaa
    54541 gcccagcata ataccggtaa cgatattaca agtcagaacg atgaacacgg cacacaccag
    54601 gccagccatt gcgtcaataa aatcgttgaa atccagcttc gagacgttac ttaacatcag
    54661 caaacccacg tacatcagag cgggtgcagt ggcgtaacca gggatcaaaa atgacagcgg
    54721 tgataagaac agaatcagca ggaataacgc ccccactacg gttgcggtca accctgtttt
    54781 acccccggcg gcggttcctg ccgctgattc gatataaacc gctgcgggcg ctgcgcccac
    54841 cagaccggag aatattgaac ttactgagtc actggtcagg gctttgccgc cattgatgat
    54901 ctggttgtct ttatccaaca aattcgcctg accggcgacg gcacggatgg tgccagtggc
    54961 gtcgaacact gcggtcatca ccaatgccag tacactcgga agtacggttg gctggagtgc
    55021 gcccataata tcgaggctga aaatcagaga cttaccatct tcgccagtca gacttggcat
    55081 cgccaccaga ccgtggtatt tcaccgctgg gtcaaagatt aagccgataa tcgaaattgc
    55141 gataatcacc aacaagatcc cgccgggtac gcggcacttc tccagaccga agatgaccgc
    55201 cagccccagc aagctcatca tcaccgggaa ggaggtaaac gcaccgagcg ccactggcaa
    55261 gccttcaatc gggtttttga taaccatacc cacaccgtta gcagcaatca gcagcagaaa
    55321 caggccgata ccgatacctg taccgtgagc gatacccatc ggtaaattac gtaagatcca
    55381 ggtacgcaca ccggttacgg aaatagcggt gaagatgacg cccatcagaa agaccgcgcc
    55441 cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg taaatgccgt
    55501 caaggaaatc gcacaaccaa tcgccattgg caaattggcc cacaacccca tcagcaacga
    55561 gccgaagccc gcgaccagac aggtggcaac aaacacagct gcgggaggaa aacctgcttt
    55621 gcccagcatt cccggaacga cgataacgga ataaaccatg gccagaaagg tcgttaagcc
    55681 agccagtact tcctgacgaa cggtactgcc acgagcggta attttaaaat aattatccag
    55741 cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg tctccagaca taatgatgtc
    55801 ctcacgagga tgttaaagtt ttattattag ttgcgttcgt acaccagacg accatcaacg
    55861 taggtgcggt agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact
    55921 aaagaaacag agttgtcata gcgcagctgc tgtagcggag tggcggtggg ttccatcacc
    55981 acgaaatcag cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag gcccagagat
    56041 ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg atagccttgt
    56101 aattgcaata ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt ggttccggca
    56161 ccgatatccg ttcccatgcc cactttaact ttcttctgcc atgctttttt caagttgaat
    56221 aagccgctgc cgaggtaaag gttggaggtc ggacagaaag caatgctgga tttggtttcg
    56281 ctgagacgat cccactcttt ttcttcgaga tggacgcagt gagcaaagac acagttttta
    56341 ccggtcaggc cgtactgatg gtaaacatcc agataaccat catggtcagg ataaagcgat
    56401 ttcacccagg caatttcatc tttgttttca cagagatggg tatgtaccca cgtatccgga
    56461 tattcttctt tcaggcgttg cgccatcgcc atctgttcag gagatgaggt cggggcgaag
    56521 cgtggcgtaa tcgcatatag cagacgacca tttttgtgcc agcgttcaat cagttctttg
    56581 ctttggtgat agctgctttc ggcagtgtcg agcagataat ccggtgcgtt gcggtccatc
    56641 atcaccttac cggcaatcat acgcatattg atatgactgg cggcttcaaa cagcgcatca
    56701 acagattgcg gatgaacagt gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc
    56761 tgcttgatga agaatgccga catttcgcgg gcgtactcta aatcctcata acgacgttca
    56821 gtagggaagg tgtgtttatt caaccactcc agcaattgtt caccataggc ccccaccatt
    56881 tcactttgcg gataatggat atgtgtatcg acaaagcccg gtactatcag tttgccgcga
    56941 tagtcgcgca cgcgaatagt gtcaggaatt tgatgctttc cgtcttccca ttcgccaaac
    57001 cattccactt ttccctgttt aatgagtaat aaaccatcct caataaaccg cagcgcagag
    57061 gcaatctctt ccgggttatc gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct
    57121 ttcaacgtgt gttctcctga catcattgac tcctttactg ttaatccatc tcttccgctg
    57181 aggtaatacc aggcagaacg ttttcctgtc ggtagccacc agggaggata atatttaata
    57241 aaatcgcagt taacccgcca gcacaaatag ggttttcaac tagtacataa atagaggctg
    57301 gcaatatttt aaaaatttcg ggatcgtagg agacgccaag tcctaaacct aaagaagtgg
    57361 cgacaataag tgtttctcgg cgctttaaac cgttggtgat gattatgcga atcccagcga
    57421 tggcaatcat ggaaaacatc aacgtcattg cgcctcccag aactgccgag ggaatggtcg
    57481 tgaagaagcc gccaatcatc ggaaacaaac cgaggataac cagcattacc gcgatggttc
    57541 gcccgacata acgtgaagcg acgccagtca tctgaataac cccattattt tgcgcaaacg
    57601 tggttaaggg taatgacccg acagcggagg cgataacaga aaccagacca tctgccagca
    57661 cgccgccttt cagtcgggac tgatactctt ccccctgaat ggggcggcgg gaaaccattg
    57721 ccgtggcggt gatatcgccg actgcttcca gcacgctaag cagataaatc gtgccaacca
    57781 ccaggaactg atggaagcta aaactaaagc cgtatttgaa cggatgcggg atggtgatta
    57841 acggcaaatt gcgcatactg ctgaaatcca ccatgcccag gcataacgat gcgatatagc
    57901 cgacacatag cccaatggcg atccctccca tgcgtagcaa cggactgcga cagcagttaa
    57961 agccgatcac cacaattaaa accaataaac caacgccgag atgttcgtaa ttgccgaacg
    58021 taccgctgct tttggctgca aatcctccac caaaatcgat aatgccgact ttaatcaggc
    58081 ttaagccgat catcagtacc acaataccgc tgacggtagg cgtaataacc cgacgtaaat
    58141 agggcaagat aaatgaagat ccgacaacca gaaatgcgcc aacgaaggag acgccgagaa
    58201 gcgacgacat gattaactct tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa
    58261 tcatgaccgt aacaaatgaa aaattgactg actgaattga gagtaggcca gaaccgacga
    58321 tgccgtagcg gtttacttgt aaccaggtac caataccaga ggcgatcatc gccatagaaa
    58381 caagataggc ggttgtttca gcggaaagct gtaaggccgc acccacgatt aacgcggggg
    58441 ttaccatcgg aacgaaaatt gccaacagat gggtaatggc accaacgaga gcctgatgaa
    58501 agggagggcg atcctccagt tcaaatataa ggtcagaacc tgcatggttt atatcagaca
    58561 tcccttcact ccttttaaag taaatttgat catctgaatt agtaaaataa attcccacga
    58621 atttggggcg caggacgcct tgagaacggc ttatctcatt ttcgcagtca ctatttagtc
    58681 atcgttcaaa cagagaaaca ggccgccgaa gcggccattt attacttata ttttttccag
    58741 cgcggtgagt attttctccg gtgtgaaatg ccattcgcgt aaccagatgc cgcaggcatc
    58801 gtgaattgcg gtagcaatcg ccggagctgc gccatttaca ccaatttccg agatcgattt
    58861 cgccccgaac gggccgactt tatcgtcgct cggcaccaac acagcgcgga aatcacgcgg
    58921 aatgtcacca attttcggtg cgccgtaact gcgtaaatca cgcgttaacg ggtgaccttc
    58981 ggcgttataa atgatctctt cgctcatact gtggccgata gcacgcaggg tggcaccgta
    59041 gatttgtccc aacgctaact ctggattgac cggtgtaccg cagtccagca aggcgtagaa
    59101 tttatccagg cggatttcac ccgtacgcgt gttgacggca acttcagcga agtttgcgcc
    59161 atacgggaaa gcgaaatcag gcgtgatata actgccagtt cccaccagtg aaccaaagcc
    59221 ggtgccggtt tcgcctttat gggcaatatc cccgaaacta acttcgcctt tcttgccgcg
    59281 cacgacgccc ggcgttgcta gttgaacatc tgccactggc tcacccaaca tttgcgcgcc
    59341 gtggaacaga attttctccc gcagattttc cgctgccaaa cgcgccgcgt tacccgagaa
    59401 gcaagtaccg gacgaggcat atgcgccttt atcaaacaac gcatgatcgg tatcaccgga
    59461 gataacatgc acgtcctgcg gtgggcagtg cagcacttct gctgccagtt tcgtcactac
    59521 agtatccaga ccagtaccaa tatccgcacc gccagaatga acgataaagg tgccgtccga
    59581 ttccagtttg atcatgcagt tagcctgatc gatatccggg atacccgatt tctgcatgat
    59641 aatggcgacg ccgcgaccga tgtgccagtc actattttgc ggtttcggtg aagaccattg
    59701 gatcatctcg cgaccctgac gcaggatctc ttccagtgcg cagctggcgg cggaaggaac
    59761 ggaggtcggc gctttacctt caccgattgc accgagaatt ttcagctctt gcccttcgtg
    59821 tacccggtta cgttcgataa tttccagttg gtcgatctgt aactgttcag ccagttccgc
    59881 taatgccatg gtgatagcga agttaccttt cggtgcgcca taaccctgat aagcaccatt
    59941 tgggcaaatg ttgctgtagt aggtggtgac ctggaaatcg acgttatcgc acggatataa
    60001 cggcagcgac agcgccggtc cgttacacgg tacggtgagt gagtggttgc cgtaagggcc
    60061 agtgttggcg cggaaatcca tcttcactgc cgtcaggcga ccatcttttt tcgctcccag
    60121 tttgacggtg actttcgcga cgtgacgaga ggtgttagca ataaactctt cttcacgggt
    60181 gtagcggaac agtaccggac gcccggtcac gcaggttgcc catgcgcaca cttcttccag
    60241 caggatgtcc tgtttggagc caaaaccgcc gccaactcgc tctttaatga catgaacttt
    60301 atgctgtttc atgcctacga ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt
    60361 ggaggcgtgg ataaccagac gatcgccgtc catccgggta aagcagatat gtgtttcagt
    60421 cgggcactgc tgtgcctgcg ttgagttata ggttcgctca atgatcacat cggcatcggc
    60481 aaagcctttg tccatatcgc caatatgacc atgaatactg gcggcgatat ttttgcgagg
    60541 gcgagaaccg atcgggaagt tgatgatcat atgctcgccg cgctgggctg catggctgtt
    60601 atcgtcttcc agagtatctg gcgcaccagc aacatacacc accggttcat cgtgcacgac
    60661 aggcgcatct tccgccattg cttcgtcgat cgacattacc ggcttaagca cttcatattc
    60721 aacgtcgatg agcttcaatg cttcgagcgc aatttcttca ctttctgcga cgaccgcagc
    60781 aacacgatcg ccgacgtgac gcattttctt gccgaacata cggcggtcaa gcggtgacgg
    60841 ttccggtgcg ctctgaccac ccggggtata gtagatatcc gggcaattca ggtgagtaat
    60901 aacgtgaacg acgcccggta aggcttcagc tttgctgaca tccagatgag taatcagtgc
    60961 gtgagcgtgt gggctacgta acattttaat gacgcaggcg tcagccgtta cgcggtcttc
    61021 aacatagcag ggtttcgcct gcaccatttt cgcggcatca gttttaggat aatgcttgcc
    61081 aatgacttca aggtcgtcac ggaaagtcgg agcgatatca atggtggcct gcggattatt
    61141 tttacgtgca accgccagtt caatgacctg atagtattgt tgccagcctg catcgcggct
    61201 gaacaaacca gaaagcgcgt cgtcgatctc ttcgcgggta ggtgcggcga tgcgatcgag
    61261 aagatcggtg ataatcagag ctgcagctgg atcgttataa ccagactgca ccacgccaac
    61321 atcaaccatt gcctgttgaa ccagacttaa ctcgttccat ttgcccaaag attctgcggt
    61381 acgaatatct gccttctcta actgtgcggc aataagcaag gacgcgttaa cgatattacc
    61441 gttaaagatt attgcgtcag aaccggcaaa cccgaagcca tcatcactgt tgcgtacaga
    61501 gtgcattccc atgttaaaca acagcttttg cacgttttcg cctggattaa cggttagctc
    61561 ctgaggcgcg ccatttaaag taaagtggat gatcatacgg cttcctcccc agcctgttgg
    61621 cagtcggcat acaggtcggc taccagaact cccgtgatat agcgtttata ggccacgctg
    61681 ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag cctgttcaag tgcattgcct
    61741 tccagatttt gtttttcgac atcatgcaga cgcagtgctt tactggcgac gccatccagc
    61801 gcaattcgca taccgtcgtg gtctgtcatt gcaacggctg ccgtcacgac ggttaaacct
    61861 gcctgagaac ggctaatttt gcgggtcgca caggtgcgat acggatcttt aatgataatt
    61921 tcggttagca ggcgatcgca tgggcaggcc aggtagtcct cgattgacag cgtttcgccg
    61981 ttgccaaaaa ccagttcagc atccagtgcc aacaggacgg gaagcagcac cgactcttcc
    62041 tggcgggcgg cgatttcacc accaatggtc gactgattac gaacatggcg tgagtaaaca
    62101 aaaccgaggg cttcacgcag cgctgcagga ataaatcgcg catcacgcag tggctgcaag
    62161 cgagacattg cgccaatccg cagtgcaccg ttatcccagt caacccagtc cagttccaga
    62221 tcctgtaagg aaatggcaat ctttttatcg gtacgggttg gtgtagcatt gagtttgctg
    62281 cccccggcga accagacggc ttcatcctgg tagcggcgct tcagttccag cgcctgttcg
    62341 acagagtcgg gcctgaaaaa ttgttcaatc atcttctttc cttatttaac cctcttccct
    62401 gcgggaagag ggcatctgtc atttatgcca gcgcatccat ccgacgccac atactggcag
    62461 cggcttttct ggcttgtgca taaatggaat cgcaatcgaa gttaaactga cggtcttcat
    62521 agaccatcac accattgacc atcacgctgt gaacgctgcc tgagcccata ccgaaagcga
    62581 tatgcccggc gatattgtct gccagcagcg gcgtcggtga gttgtaatcg caaatggtta
    62641 aatcagcttt gtaaccggct tccagaagtc caaatttcgc gccaaagttg cggctcatca
    62701 gttcgttacc gttagtcagg gctttggcaa aactgtcagg ccacagcgga ccacccgcat
    62761 cgcgatgttt aaagaaggca aatttcatct cttcaaacat gtccgaacca atgccgtccg
    62821 ttcccaacgc cagattgcgg atgtcgctaa ggtgatggtt gtagccgaca tggttgttca
    62881 tgtttgaacg ggcgttatgc accaggaacg catcgcgctg attgagtagg gcgatgtcat
    62941 ctttcgacaa gtacagccca tgagcgacca gcgttttgct atcgatgaga tcgaattgcg
    63001 ctagtcgtgc cagcaggtct ttgccgtacc agtggtgact gtaggaaacg tcgtaaaggt
    63061 cttccgcagc gtgaatatgc aaaccacggc ctgtggcttt cacggcttca cgtagcatct
    63121 ccagaccggc atctggcacg gtaaacggcg cgtgagcacc gatatgtgct tccaccagat
    63181 acggctcggt tgctgctttc ttcgcctcat caatctgacg ggcgaagcgg atgttttctt
    63241 ctacaccttc ctgcaactct ttgatgccgt tgttacggtc agtagtttca aaacaggtca
    63301 tcgcgcgcag gccaactttt aaaaatgcgt cgcgcaatgt ggagagcgac ccgccgatat
    63361 acgccggaga ggcatggtga tcgataaccg atgtacatcc gctcttaatc gcttccaggg
    63421 aacaaatcag cccgctgtaa tagagcgact cttcatcaag ggcgcgatcg agccgccacc
    63481 agagattttt cagcgttgag atgaaatccg ggcagggggc gatgtttgcc ataattccgc
    63541 gggaaagccc cgagtaaaaa tggttgtgcg agcagacaat tcccggcatc acaatccgac
    63601 catgcatctc tttgtagctg gcatcggggt agcgttgcgt cagggcatcg ccgatagcga
    63661 caatcacatc attttcgata gcgatatcaa cgccttcctg cactttcgcc gggtgtaact
    63721 gcactgcggt gacattcttc agaatcaaca tgattacacc tccacgcggc ccagcagata
    63781 atgatgatgc tggtggacat ggctgatgat gcggcacata tcgttcagct ccggtggtac
    63841 gttgttgaac tgaccttcgc tgtcgatgtt taacacccag ctttggttat tcagtcgtac
    63901 tcgtacccgg caatcttcca caaggaagcc tgggttgctg ctgttatcaa agtcttgcgc
    63961 caggctgaaa acggtgattt tgtctttgta tggtttaccg ttccacggac agaactgagc
    64021 gcagttgccg cattcgttac agtaagcgtc gaggtgcagc gtctggaatc tattctggaa
    64081 gcctgggacc gcaatggaga cgttggcgcg gttcgggcag acatccacac acttgctgca
    64141 aacgtagtta cattcgaggc agcgagcggc ttcctgggcg acaaacgcgt cacgatcgtc
    64201 actgttcacc agagtgatag agatatcgcc tttacgttga tagatttccg ccggattgac
    64261 gttgttccag tatttatcgt tctggtggga acggatattt tcccgactaa ggatggcatc
    64321 ggtcgcccga cgcgcggttc cgacagcagc gacaatggag gatggtccgc gctgtacgtc
    64381 gccgatcata aagacgtcag tcagacgagt ttcgccattg tggtcgacgt ctggccagcc
    64441 gtttttgtcc agcggcacgc ccatcgcatt cagggcttca gtatcctgct gttcaccaat
    64501 ggcggtgatc aggctgtcta catgcagtgt tactgtttcg ttggtttcaa ccggacggcg
    64561 acggcctttc tcatcgggtt cgccaagcga cataacgcgc aaggttaagg tgccatcagc
    64621 atcgaaacgt tccggattat tcaggaaacg gaactccacg ccgtcgtgca acgcttcttc
    64681 atactcttcg cgccatgcgg gcatctcttg cagtgaacga cggtaaacga tcgttgcttt
    64741 ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc attgcggtgt tacccgcccc
    64801 gacaacgacc acatgtttgc ccagcttgag cgctgtaccc ttgttgtatt cacgcaggaa
    64861 ggggagtgat ttccaaacat tttggttgtc acccgccagt ttcacaccgc tatttttatc
    64921 agtgccggtg gcaatcagaa catagtggaa gccctgattt tttaactgtt caacggtcaa
    64981 atcgggtgag cagccatact caaatttcac gccgtgatcg gcaacaaaat cgatatcgtg
    65041 ctgaattaac tcagccggaa ttcggaactg aggaatgata tttttcacca cgccgcccgc
    65101 attggcttcg cgttcaaaca gcgtaaccgg atggcccgct ctggcaagga agtaacctgc
    65161 tgccagaccc gccggacctg caccaatcac ggcaaccgga tggcgtgaac cagaaccggc
    65221 tggtttgtgc cagcgttgct tatattcatc ccaacctttt tccagcgcga cttttttcag
    65281 ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta cagttgtatt ggcactggtg
    65341 atcgcaaata tgaccggtaa tggcgggcag agcgttgcgt tggtagatga gttccagcgc
    65401 gtcggcatag cggtgttcgc caagcagacg gatgtattcc ggaatatctt gcttgatagc
    65461 gcaggcagta acacaggggg caacgtagca atcggtcagc ggcaggtctt ctgccacttc
    65521 aatacgctct tctggcttcc agtgtttctg ggtgtattcc atggttaacg catctgctgc
    65581 cagtctgttc agtcgttcga cgtcaacatg gtcaagtccc caggcgtcgg agccttccag
    65641 ctcgcgcatg caggcactta agcgcaaata gccgccaggt ttcagcaggt cggttgccat
    65701 agtaataggg cgaatacctg tatcaaaaat atcgcggata gtcagctgac tggcaccacc
    65761 ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag agaactgctg caacattgat
    65821 ggagagcggg aacagcgcac ggcctgacat atacatctct tcaccaggca gtgcgccttt
    65881 attgttgatg gtgccgagag tgttagtcag ttttacgcca aagccgagtg atttttcttt
    65941 tgccagtgcc atcaggcgtt ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc
    66001 aaatgactct tcttttaagc ctatgtaacc gaaaccgcag acatcgagga tctcacgaac
    66061 acgcgcgtat cccagtaagg tcgggttaag tttcacaaag gtgttgagcc ctttttcttc
    66121 cagcatgtag cggcaaatgg cttcgatttc atgcggagga cagccgtgca tggtggagag
    66181 ggtgacgccc tgcaccatac tggtggggat gcgagcgggt aaggcttgca agctttcgcg
    66241 tttttcctgc aatccgtggc gggctaaaaa tgcgtcatcc tggagtaatt tattcagcgt
    66301 atcgcgatat tgcgcgaatt tcggatggtc agatgcgtcc atcatattgt cgatgaactg
    66361 ttgcattggc ggttgcttaa taccttcgag gttgtaaccg acgctcatat taaagatgaa
    66421 cgatttaccg gaatcagaag gctggaacat cgcttcgaga aggtgcaggg caaaccaggc
    66481 tttgaggtat tcatcccagg ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa
    66541 gcactcgtct tcggcatcga tacagggctt ttccagctcc aggcggtcaa gaatttggac
    66601 ggtttttagt tcgatgaagc gtccgccagt cagccaggaa gtgacaatat tttgcgcgag
    66661 ctgcgtgtgc gggccagcgg cagggccgac gggagtggca caggtttcac cgaagacgct
    66721 aacagtttta ccttttacag gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg
    66781 ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat
    66841 aatatccccc ataaccctct ccttagctta tacagatatc attgagtgac cggtgttgag
    66901 aaatccggtg gtttaggggg atagagcaac aatcgcacca ctcttgccag gtttggcggg
    66961 aaatataaaa aaatgtgagg cggctcgaaa cttaaaggct ttttgtgaat gaactcacag
    67021 gtgccaaatg agaattaaaa tcactcctga aaattattat tgtcagccca tttatcactt
    67081 tttctctttt ctcaaaatca gtgattcctt atcattatga taaagagtgc tggagagatt
    67141 aaatatttga acgcaatcgt ttaactacat tttctaaatt ttagtatacc ttttttgcgc
    67201 tgtaataaag tcgtccggag tatcaatatc taaaattatt tctgcatttt ctatctcgac
    67261 ggaataatga gctcccataa ggagtgcctg acgcatattg gtgacattgg gtcgttggat
    67321 tgcctgcatc aagcatggtt ttgacactaa aatcggatgg ccggggatgc cattatggag
    67381 tggcagtatt gcgccatcat ttcgtaacga ccagattttt ctaaaaatat cgatggtgag
    67441 ggttggcatg tcaccgtggg tgagaaaaca atgttctgtt tgcactgcgg gtactgcggc
    67501 ctttactgat gttagtaaac cctgcgcata atctgggttg tggataatag tgatattgct
    67561 ctggttcgca taacgttcgt gcagttcatt accacgatag ccggtgacta aaataattcg
    67621 gctacaaaac tgcaacgcat ttttgatact tgtatcaaga attgtcccct gttgccaggg
    67681 taacatcatt ttccattgcc ccattcttga tgataatccg gcagcggtaa ttatacagtc
    67741 gatggctgac atagtggttc cattatttat aggggctaat aacgcctgat aattgcgtta
    67801 cttcctgatg gattaagttg gggataattt atcccagaga ggtcataaag actcgtgaaa
    67861 agtatagttg acccatcggc attagtcatt gatttaggtg cgcagaaacg tcccactgtg
    67921 atttctgttg ttggtgctgg ggggaaaacc agcttgcttt tttggctggc agaactgctc
    67981 caggctagcg gcaggcgtgt gttaatcacg acgacgacac atatgtttat gccaacgtct
    68041 cactggcccg tggttttctg tcgtgatccc gccatgcttc ctcatgcgtc tcttacatcc
    68101 cccatttcat tttgttttca cagctggaaa gcgaaccagg gaaaagtgca gggatttacg
    68161 ccagaagcga ttgatgcact ggtgcaacga ccagagtgtg acgtaattct cattgaagca
    68221 gatggctcgc gtggaatgcc gttaaaagcg cctgatgagc acgaaccttg catacccaaa
    68281 agcagttgct gcgtgattgc tgtgatggga ggacatactt tgggcgcgaa agtgagcaca
    68341 gaaaatgtcc atcgctggtc gcagtttgct gatattaccg ggttaacacc tgatgcaacc
    68401 ttgcaactga gcgatctcgt tgcgctggtt cgccaccctc agggggcgtt taaaaacgta
    68461 ccgcaaggtt gtcggcgagt ctggttcatt aaccgttttt ctcaatgtga gaatgcgatt
    68521 gcacaaagcg agctccttca accgctgcaa caacacgacg tagaggcaat ctggctgggc
    68581 gatatacaag aacatcctgc aatcgcgcgc agatttgtga attagcggcc cgggtattcc
    68641 gggttactga aaggatatcc attgaggttg gtatgaatat tttcacagag gctgcaaaac
    68701 tcgaagagca aaattgtccg tttgcgatgg cgcaaattgt tgatagccga ggctcgactc
    68761 cccgccattc tgcacaaatg ttagtgcgcg ccgatggttc tatcgtcggt acaattggtg
    68821 gcggaatggt tgagcggaag gtgattgaag agtcacttca ggcattgcag gaacgtaagc
    68881 cgcgattatt ccatggacgt atggctcgta acggtgcgga tgctgtcggg tcagattgtg
    68941 gaggtgcaat gtcagtattt atcagcgtcc atggtatgcg tccacgtctg gtgttgattg
    69001 gcgcggggca tgtcaaccgg gcgatagccc agagtgcggc gctattagga tttgatattg
    69061 ccgttgccga tatttatcgc gaaagcctca atcctgaact attccctcca tcaaccacgc
    69121 ttctccatgc tgagtcgttt ggtgcagcag tggaagcact ggatattcgc cctgataatt
    69181 ttgtcctgat tgccacgaat aatcaggatc gtgaagccct cgacaaactc attgaacagc
    69241 ccattgcatg gttggggttg ctggcaagtc gccgcaaggt tcagcttttc ctgcgtcaat
    69301 tgcgtgagaa aggcgtggct gaagaacata ttgcccgttt acatgcgccc gttggttaca
    69361 acataggtgc ggaaacgccg caggagatcg ccatcagcgt gctggcagaa atattacagg
    69421 tgaaaaataa cgcgccgggt gggctgatga tgaaaccttc tcatccttcc ggacaccagc
    69481 tggtggtgat tcgcggtgcg ggagatatcg ccagtggtgt ggcgctacgt ctgtatcatg
    69541 cgggttttaa agtgatcatg ctggaagtgg aaaaaccgac agtgattcgt tgtaccgtgg
    69601 cgtttgccca ggccgtgttc gatggcgaaa tgacggtcga aggcgttact gctcgcctgg
    69661 caaccagtac tgcggaagcg atgaaactta ccgaacgcgg attcatccct gtgatggcag
    69721 atcccgcctg ttcattgctt gatgaactga aaccgctttg cgtggtggac gctattctgg
    69781 cgaaacagaa tttgggaacg cgggcagata tggcaccagt aacaatcgcg cttgggccgg
    69841 gctttactgc agggaaggat tgtcatgcgg taattgaaac aaatcgcggg cactggctcg
    69901 gtcaggtgat ttactctggt tgtgcgcagg agaataccgg tgttcctggc aatattatgg
    69961 ggcataccac ccgacgggtg atccgtgctc ctgctgcagg cattatgcga tccaacgtga
    70021 aattaggcga tctggtgaaa gagggcgatg tgattgcctg gattggtgag catgaaatta
    70081 aagcaccgtt gacggggatg gtgcgtggct tgttgaacga cggcctggca gtggtcggtg
    70141 gttttaaaat tggtgatatc gatcctcgtg gtgaaacggc tgatttcacc agcgtttctg
    70201 ataaagcccg ggcgattggc ggcggcgtac ttgaggcgtt aatgatgttg atgcatcagg
    70261 gcgtgaaagc gacaaaagaa gtgctggaag tggcttaaat gaaaatggcc ggatggtatt
    70321 acccacccgg ccttaaaatt actgacaaat caccgttccg gttttgcctt caatcccctc
    70381 tttcgctttg ctcaatacgg taatcagtgc ttcacgacct gcgcgagagc gagcaaacga
    70441 ggcggcagct tccactttag gcagcataga acctttcgca aaatgacctt cttcgataaa
    70501 gcgttcagca tcacttaatg acaggcgatc gagccactgt tcattctctt taccgaagtt
    70561 aatggccact ttttctaccg cagtcagaat gatcagcata tcggcatcaa tcatttctgc
    70621 taaacgggcg ctggcccagt ctttatcgat aaccgcgctg gcaccacgca gatggttacc
    70681 ttcacgaata acaggaatac caccaccgcc aacggtgatc accacctggc ctgcatctac
    70741 cagagcttta accgtttctt tttcaataat atcaaccggt tttggcgagg caacaacacg
    70801 gcgatagccg cgaccagcat cttctttcag agtgtaaccc tgttttgtca gttgctctgc
    70861 ttcttgctcg gtaaagaacg aaccgatcgg cttggtcggg ttgaggaatg ccggatcgtt
    70921 agcgtcgact tcgacctgag taaccagcgt tgctaccggt ttattaatgc cccgagaaag
    70981 cagttcttct cttaaggcgt tttgtagatc gtaaccaata taaccctggc ttaaggcaac
    71041 gcagacagac atcggcagca ttggcgagtg cgcttcagtt tttgcagcag cttcaaaggc
    71101 ctggttaatc atgcccacct gcggcccgtt accatgagtg acgataactt catgtccttg
    71161 ggcaattaaa tcaacaattg cctgagaagt aatttttacc gctttcattt gtccggccag
    71221 gtcatcgccc agcgcattcc cgcccagggc gagaacaatt ttcttactca tttttcctca
    71281 ctcatcattc ttgattgtgt atagatccct gacagtggtt agagcacggg agggacaaac
    71341 ggtttgcgtc gcaggaaacg acctcgcccg gctttgcctg taaaggtgcc gtcacagaaa
    71401 atcgtttcac cacgggataa ggttctgaca atcgcgccct gacaggtaaa accctcccat
    71461 ggcgagtagt cggcgttgtc gtggagatgg cgatgctgaa tttgttggct ctgacgtggg
    71521 tcgataatca ccacgtcgcc atcggaaccg ggcgctaata atcctttttg cggccacagg
    71581 ccaaacagcc tggcgggcat tgcgctggtt aattcaacaa agcgttccgg tgttatacgt
    71641 cccgtcatca cgccactgga aaacagtaac tgcatgcggt tttccacacc gggtaagcca
    71701 tttgggcagc gactgaaatc gcctttagaa atttgcaggc gttgagccat cgagaaggtg
    71761 cagtgatcgg tcgccaccac gtcaatcgca ccatcgctga tgccacacca cagtttgtcc
    71821 tgctcgcgta cgttacgcag cggtgggcta agaatgaact tcatgccgtc ttctgtatcg
    71881 taactgcgtt cgtccaacag gagatattgt gggcaggttt caacccagac tggctggtga
    71941 ttcgcacggg caagacgcag ataatccaga cctaagccgt tagacaggtg cacgatatag
    72001 agcggggcgt taccggcaat ttgtgccagg ttaatcatgc gggcgatggc ttccgcttcg
    72061 cattccagag ggcgactcaa ggcatgatag cgcggcgcgg ttaaccctgc ggcgataaat
    72121 tccgcccgct tgctggcgat agccgcatca ttttccgggt gcacggtggt cagcgcgccg
    72181 gattcatgca gacggcgtaa tgcctgcaaa acctcgtcat cgttgagttt gtattgatag
    72241 gttaaataga gtttaaaact gctcagtcct tcctcgacca tcatcgggat ttcgtcgagg
    72301 attgcgtgat taatgtgctg gatcacaccg tgaaagctgt aatcgatgac cgctttatgg
    72361 gcggcataac cacgataaac ctccagttga tggcgtaacc gacagccgtt tgggccaaat
    72421 cccatatggt caataatggt tgttgtaccg ccacacgcag ctgcgcgggt accggtaaaa
    72481 aaatcatcac aactgcgcgc gatgccgaca tcaatattga aatgcgtatg gacatccacg
    72541 ccgccaggga aaacgtaaca gccagtggca tcaatttctt catacgggag ctgcggcgaa
    72601 atattgttgc ccaactggcg aacaatcccg ctttcaatca gcaaatcctg tttggcttgt
    72661 ccatctgcgt taacgacagt gccgtttttg atcaatacgc gcatagcaaa ctccaaaaac
    72721 cctccggcga accggagggg aataagtgaa gagtaattac tctgttgcca gccagcttaa
    72781 cgggattgca gcgtacatcg cggcacaggt caccaggtga gatttccagg ttttttcgtt
    72841 aggtgcatgc gcttcaggtt ctttacccgg gccaaagccg atcaccggaa tgccgtgacg
    72901 gcccatgata gatacgccgt tagttgagaa ggtccactta tcaacaaccg gcgctttgcc
    72961 aaacagccct tcgtaggcat tcaccagtgc tttaacggtg aagtgatctt cttccacttt
    73021 ccaggtcggg aagtagcatt cggttgggta aaccaggcca gtccaggacg gacggtcgta
    73081 gttgtacata gaaacaaccg cgttagcttt ctgtactgca ggcagggcgc ggatttcgtc
    73141 cagcgcgcct tcccaggttt cgccccaggt cagacggcgg tcaatagaga cggcgcagct
    73201 atctgctaca gcgcaacggc ttggggatgt gaagaagatt tcagaaacgg tgagggtgcc
    73261 tttgccgagg aattcgtcat aacccagacg ttgggagagt tcttgtaatt cgccaagaat
    73321 cggacccatt ttgaaaatgg cgttgtcacc gcgttctggc gcagaaccgt ggcagctaac
    73381 accctgaaca tcaatacgaa tttccatacg accgcgctga ccacggtata tctggcagtc
    73441 ggttggttcg gtactgacca caaattccgg gcgaatgccg gattgttcaa taatgtactg
    73501 ccagcacaga ccgtcgcagt cttcttcctg cacagtaccg gtaaccagca gggtatattc
    73561 atcttccaga ccgaggtctt taatgatttt accggcataa accatagatg ccatgccgcc
    73621 ttcctggtcg gaagtaccgc gaccaccgat tagctcatca gtttccatgc cttcgtacgg
    73681 atcgaagtcc cagtttttga tgttgccaat gccgacggta tcgatatgag cgtccattgc
    73741 caccagacgc gggccgtggc cgatataacc gagaacgttg cccatcgggt cgatttcaac
    73801 tttatcgaag ccgacttttt ccatctcttc tttaatacga tgtactacgc gtttttcgtc
    73861 gcagctttca ctgggaatag caaccatgtc gcgcaggaag cgagtcatgt ccgcctggta
    73921 atcttttgct ttttcaagaa tcagtttgaa tggaatattc ttagccatta tttttctcca
    73981 gattttttac tccccgtttt gcagggagta gttccaatag agtgattaag gtgctacagc
    74041 gtgtttgcct tcccagacaa cttcgcggta gtgcttcacg tcggtgtcgc cttcagtgct
    74101 gataaccagc accacggcat ctttgttcag cgccagtttt tccatcaggc tttgacgttg
    74161 cgggtgataa tgaaccgctg cgagaacgcc caaaccgaca gcgccggatt caccggagat
    74221 gatgcgcggg tcgttgccgt acggattacc cagcacgcgc atacctaatg cggcaacgct
    74281 gtcctggcag gagatgaatt gggtggcgca gttacgtagg atttcccagc ccagcgggtt
    74341 aggttcgcca caggccaggc ctgccatgat ggtggccata tcaccgccaa cgttgacgat
    74401 gtcgcctttg acgccggagc gataaataca gtcagctttg tcaggttcaa caataatgct
    74461 gtgcagattt tgcgggctat agacgtcgac cagataaccc agcacaccac cggccattgc
    74521 tccgacaccg gcttgcagca gaacgtgcgt cggggttacg cccatttcac gcatttgctc
    74581 gacggcttca tctgccaggg ttgcgtagcc ttgcatgatc caggttggga ttttggtgta
    74641 accttcccat gccgtgtcct gtaccacttc ccagccgtgc tgctgcgcgt gttgcatggt
    74701 caggcgaacg gtatcgtcat agttcatatc tgtgacgatg cactcggcac cgaggttcag
    74761 aatggcgtca acgcgttcct gagcagaacc tttcggcatg taaatcaccg cattctgtcc
    74821 gagttgctgc gctgcccacg ccacaccgcg cccgtggttg ccgtcggtgg tggtcgcgaa
    74881 agtcattttt tcgccgatgg catttttcag gtgctcaaat gacagcgttt cgatatcaag
    74941 atgatatttt tcacacaata attgagcgat agcgtacgca ccgccgagca ttttgaaggc
    75001 gttcagaccg aatcgttttg attcgtcttt gacaagaatt ttcttcaccc caaaaaggtt
    75061 tgcgagatcg tccagcgcac aaagcggtgt tgggcgataa ccagctattt tctggtggaa
    75121 ctggcgcgcc agtttggcct ggctttgcga aaagagcggt gatgtttcgc cgttgaaaaa
    75181 tttgttatcg gcgatatcaa tcttcaatga gaaaacggac atatcctatc cttttggcct
    75241 ctggaaatag agggccgacc agctgatcgg cccgaggggt tatttcacgc gttcttgcgc
    75301 ttctttcagc agttgctcga gcagtgcacc tggtttggcg tatttacggg acaggatcat
    75361 cgcggcgatg atataaggct tccagctggc ttctttgtag gtagcgatac ggtatttttc
    75421 gaatacgcct tcagtcactt ccccttcttt gcaggatacg ccgctgatat cagctggcag
    75481 gcagtgcatg tacagggctt cgccatcacg ggtcagttcc atcatctctt cagtacaatg
    75541 ccagtctttg tgttgcgcgt tctgtgccag acactgtttt tccagtgctt ttaagccttc
    75601 gtgatcgtta gcgcgcagca attcggtacg ctgttccatc actttgtaag gtgcccatga
    75661 cttcggataa acgatgtctg catctttgaa cgcttcttcc atgctggtga cctgacggaa
    75721 gctaccgcca gatgctttag cattgttttt cgccacttca accacatccg ggatcaggtc
    75781 gtagccttcc ggatgggcca gggtgacatc cataccgaag cgagtcatca gaccgatgat
    75841 gccttgtggt acagagagcg gtttgccata gcttggagag taggcccagg tcatggcgat
    75901 ttttttacct ttcaggtttt ccagtgaacc aaagtgttca cgtaaccacg cgaggtcagc
    75961 cattgactga gtcgggtggt caatatcgca ttgcaggttc actaaagccg gacgctgtgg
    76021 cagtacaccc tgcttgtaac cgtcatcaag tgcagcgcca acttcacgca tataggcgtt
    76081 gcctgcgccc agatacatat cgtcgcgaat accaatagcg tcggcgcaga aggagatcat
    76141 attggcggtt tcacgcacgg tttcgccgtg agcgatttgt gatttgcctt cgtcgagatc
    76201 ttgttgtgca aggccgagca ggttaagcgc ggaagcataa gagaagcggg tacgggtgga
    76261 gttgtcgcgg aatacggaaa tacctaatcc actattaaag actttggttg agatgttttc
    76321 agcacgcagt gcttttaatg ctgcggcaac gtccagtact tgtttcagtt catctggcgt
    76381 ctgttcccac gttaacaaaa aatctttctc gtgaaggtga gaggtcagcg aattgatatc
    76441 cttaatcagc tcattaacag ttttcatcat aaagtcccgg tagtggaatg acaggcaaag
    76501 tggtttattt cactgtgact tgccaaagtg attggtgttt tcacagctat acctgggact
    76561 aacaataagt gtgccagaat tatccacggg gctgcaaaag actaatttga cggataatgt
    76621 gtggggttga tcacaaattg atgccagctt ccggttgttt gtaaaatacg aaaacgtttg
    76681 gcttttttta tcgcgttatt tatttgctgc taagtatcag aaataagata aagggacagg
    76741 ctatctctct tcagtttgat aaactcaccg ccgtaattct catattgata tagaaatgtg
    76801 atatctcttc cataatttca ttagaaaatg ataaaaatct tatgttttta tcaaagccaa
    76861 tttcgcctga gttaatatca tcatgatctg tgaaccatca acgtcttcaa tgatcgactt
    76921 ttaagtaagt ctggaaggta aagctatgga gcttgctact acgcagtcag tattgatgca
    76981 aattcaaccg acaattcagc gttttgccag aatgcttgcc agcgttttgc agcttgaggt
    77041 tgagatcgtt gatgaaaact tgtgtcgcgt cgccggaacg ggcgcgtatg ggaagtttct
    77101 tggtcgccag ttgagcggca actcacgcct gctccgccac gtcctggaaa cgaaaactga
    77161 aaaagttgtg acacagtctc gcttcgatcc cctttgcgaa ggttgcgata gtaaagaaaa
    77221 ttgccgtgaa aaagcatttc tgggtacgcc tgtcatttta caggatcgtt gtgttggggt
    77281 gataagtttg attgccgtta cccacgagca acaagagcat atcagtgata atttacgcga
    77341 attttctgat tatgttcgcc atatatccac catttttgtt tcgaaacttc tggaggatca
    77401 ggggccagga gataacatca gtaaaatatt cgcgaccatg atcgataata tggatcaggg
    77461 cgtattagtt gttgatgatg aaagtcgggt tcagtttgtt aatcagactg ccttaaaaac
    77521 acttggtgtt gtacaaaata atattattgg gaaacctatc cgtttcagac cattaacatt
    77581 tgagagtaat tttactcatg gacatatgca gcatattgtt tcgtgggacg ataaaagtga
    77641 attaatcatt ggtcaattgc ataacattca gggccgacaa ttatttttaa tggcatttca
    77701 ccaatcgcat accagttttt ctgtagcaaa tgcacctgat gaaccacata ttgaacaatt
    77761 ggttggcgag tgccgtgtta tgcggcaatt aaaacgactc attagccgta ttgcacccag
    77821 cccatccagc gttatggtgg ttggtgaaag cggcacgggt aaagaagtcg tcgcccgagc
    77881 aatccataag ttgagcggaa gacggaataa accctttatt gctatcaact gtgccgcgat
    77941 tccggagcag cttctggaaa gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc
    78001 ttctgccaac ggtaaaacag ggttgattca ggcggcgaat acgggcacgc tgtttctcga
    78061 tgaaataggt gatatgccat taatgttgca ggctaaatta ctgcgcgcta ttgaggcgcg
    78121 tgaaattcta ccgattggtg ccagtagccc aatacaagtc gacattcgca tcatttctgc
    78181 aactaatcag aatttggccc agttcattgc cgaaggtaaa ttccgcgaag atctcttcta
    78241 ccgacttaat gttatcccga taactctgcc accgctgcgt gaacgtcagg aagatattga
    78301 actattggtg cattactttt tacatctgca tacccgtcgt ctgggatcgg tttatcctgg
    78361 cattgctccc gatgtcgtcg aaatattgcg taagcatcgt tggcccggaa acctgcgcga
    78421 gttaagcaat ttgatggaat atctggttaa cgtagttcct tcaggtgaag ttatcgacag
    78481 cacgctattg ccgccaaatc tgctgaataa tggcacaacg gagcaaagtg atgtaacaga
    78541 ggtcagtgag gcgcacctgt cactcgatga tgcgggcggc acggcgctgg aggagatgga
    78601 aaagcaaatg atccgcgagg cgctttcacg tcataacagc aagaagcaag ttgctgatga
    78661 actgggcatc ggcattgcta cgctctatcg caagattaag aaatatgagt tgttaaacac
    78721 ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc gttttctcgc
    78781 aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga tttcccgcca
    78841 gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc agcattgccg
    78901 tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgcgccg gatttcgcat
    78961 aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc gtgcggatct
    79021 cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg ccgtcgacca
    79081 acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt agcagtcctt
    79141 gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga aaaggcatcc
    79201 cgttaatggt gcattcgatg gtaattgttt cgctgtgatt cattgcaatt ttccccccgc
    79261 cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat gcagacgaaa
    79321 ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag attcgctgat
    79381 agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg catgttggca
    79441 gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga aattgccgtt
    79501 atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt cgcgcatggc
    79561 atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat gaaaggcgac
    79621 gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc cattaatcgg
    79681 gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa ttagcgttgg
    79741 cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt
    79801 acggatctgc ggtccagcaa tggacgtggc cgcagcacat aacgccggga gatgacgttg
    79861 agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac gtagcgagcc
    79921 atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa caatatggcg
    79981 ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg cgagcagttt
    80041 ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg cgcgatggta
    80101 agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg aactcttcat
    80161 ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc atcttcaccg
    80221 cgttacgaat agcagcggca acaggaatta ttggtggctc acccagtgac ttatgtccgt
    80281 atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc agatccggca
    80341 tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt ttagcatcga
    80401 tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg tgtacctgac
    80461 cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg atgcggttga
    80521 tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag ccaaacgccg
    80581 gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg cgttcagggt
    80641 ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc ggtcgttcaa
    80701 ccagcacgat atggcctttt atcagggtca gattcatcgc tgactgatgt agcatgactg
    80761 cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc gcaggcgcgg
    80821 caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg tcggtatctt
    80881 gagttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc accatttgcg
    80941 agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg ttgatggttc
    81001 catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc caggtgttag
    81061 aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc tggttctggc
    81121 attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga cactccggca
    81181 accctgcgct gtaaatacgt ttgcccgtga gcggattagc atctccttcg cgggcggcgt
    81241 tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca tcaagcatag
    81301 actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca gccgagggga
    81361 ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa aggtaagcga
    81421 ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg ccggtgttag
    81481 acagaacatc cagactataa cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt
    81541 caatggtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa
    81601 ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc tcttccagta
    81661 catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt acgcatgacc
    81721 agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg
    81781 aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg ctttccatgt
    81841 gacaatgttg aataacgggg gtctgatagt gcccctgtac ctggtagtcg gcggcatcga
    81901 ttgtttgttg gacattaccc gtcgacatcg tgctttgttt cagtaaattg ccaccgttat
    81961 ggattggtgc agcgtcttct gccagcgccg cttctggcgt ggtgataacg ggtaattctt
    82021 gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg cgggccacga
    82081 cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg
    82141 tatcgcgctt gttttcgtca agtgtccagg catgcccagc agtagcaaat ggaatatcag
    82201 gcacatcttc ccaggtaaaa atcgccagta cgcccggcaa acttctggct tgttcatcat
    82261 taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgca taacacatgc
    82321 ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca atggcatcga
    82381 cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa atcccctcga
    82441 tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt tacgccagaa
    82501 acgacgataa gcgttagggg aggtaggaag tgtgatgacg ttaaaaaaac acagatatga
    82561 tacgaaacag gactctgaat aggggcagtg atacgtatca atatgagtgg cgataaaatc
    82621 aatgtgataa attgggatta ttacgcatca ggatgggaaa tagggtaaaa agtcaggaag
    82681 ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa atgaaattaa
    82741 cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa gaggctggct
    82801 gctatgataa ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg gacgcctgaa
    82861 taaaaaatct ctgggtatcg tgatgttgtt atcggttgga ctgcttttgg cgggctgttc
    82921 gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg tgaaacgggg
    82981 ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagaac tggcgcgact
    83041 gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac tgggtggggc
    83101 gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgaccaaaa ccgcatcggt
    83161 tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac gttgttggtt
    83221 atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg gcaataaagg
    83281 gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag gaaaggtggt
    83341 gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac acagtgaaga
    83401 ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc aaagcgtgaa
    83461 ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc gcctgcattt
    83521 ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc cgcagggcag
    83581 caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact tgctaaggag
    83641 agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat ggtagaacaa
    83701 gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccaga atctccccag
    83761 gtggagaaga ctaaaaaagt cttctctgtc agcaacttaa acagttacct atttaatttg
    83821 cttaggagtt ctaccattgt gatccgccct tagtaactgt cacgttaagt tacacggtga
    83881 gatgacagta taacctaacg ttggcggcag ttgcacctct cacaccgcga tagcacaacg
    83941 tgaagataaa gcccatcctg gcattgtaat gtcatcgaca gactgaacac acgcatttaa
    84001 gcccgatgtg cggccttgtg gctgcgtaaa caggtttatg gtgatattgg tccttgcaaa
    84061 ccatatgcaa gctctatcag cgtttcagcc acgctcccaa cgggtacacg gttatcctga
    84121 taaggtgtga tctttctgac aatctgatga agtcgctacg ctcccgcttg ttgctgtatc
    84181 accaacgggg ctttatggtt tctattgtct ataataatct cacaattccc ctgcatgagg
    84241 ttgatatgac tgttagctac aagattactt atgagcatag agacggtgat caaattagaa
    84301 tggaagaggt tttattccat tcagagcatg aaccaacaaa agatgaaatt catgatgcag
    84361 taagaaaaga tactgtgaga tttaattctc catctaatgg agtaacggct gtttcagggt
    84421 ttaaagtgat ttcggttgtc cctgttccat gaatggttat agaaggggct ggaagcccct
    84481 aattattact tcttaatatc gccagtgcat tgatattcga tagtcacaag tgtttgcata
    84541 caaccagatg aagaaggttg tgaacattga gaagtgaatc caccaaaagg ctcggctcct
    84601 gaatatcccc aagcggcaca acgcgctttg gctgcattca tgccttgttg tggatctaca
    84661 acaggacttt caaacatacc gtaagaataa ctcatcctaa ctgtgccgtc tgatttgctt
    84721 ccaccagttg gaacgagttg tttttttgtt gcacatccag aaataatcaa tgttgaagca
    84781 agtaacagca ttaaactttt gcgcatgtaa ataatcccaa gagataatgg ttatgtattg
    84841 atttaaagcg tttttagatt aatgcattaa cttagcaaaa ttaagtcttt tctgattgtt
    84901 tgcagagtaa aaatgagttg ctttcgaaat tatttcggat gtttattgta ttacggtttt
    84961 tcaaaccgtc taactattgt cgctattgaa tgggcaataa tagcaagagg attcatttcg
    85021 tcattggagg caacaaaaag tcggtaactg atagctacaa cgaatgaagc aataacgaga
    85081 aagcggatag tgctaagatg ttttgaagat aaatgcataa cgccccctaa atgaatgtga
    85141 gggggattaa atcatgcaaa tttgttgtgt gcaatactgg taatttatcc taatagtcta
    85201 ctattcacct caatctacgt caaccaatat ttgatgtaga tttgattttc ttttaaaaac
    85261 aatatgttgc aatttgttgg gttttggggt taggttgttt tttggacgtt tccatcaagt
    85321 gtaaagatac gtttagcctt catagtgagt tcagttactg tctttttctc tgaaatcggc
    85381 gcatacgtga tcatcgctta acgagaagat cagcgccgtc aggtccatgc agcgacaatt
    85441 ctgaaatatc aggaggcatt acgcttcctg ctctttcttg atacgttgca ccgtttgtgt
    85501 gctgacactg aaatcttttg ctacgctccg aattgatcca ccattcgcga gagcggcagc
    85561 aattcccgct cgatcaattg cttttccatt accacgtttt gctatacgtc cagcggcctt
    85621 agctgcggcg atcccctcac gctggcgctc aagcatcatt tcacgttcca tttgtgccac
    85681 tgctgcaagc actgtgagca tcatattccc tgcgggagag ttcttccctg tcttaaagtc
    85741 ttctttatgg aaatgcacag acacgccgcg ctctgttaat tgctcaaccg ttgccagtag
    85801 gtcggctgta ttacggcaaa gtcgatcaat actgtgaaca tgcaaaacat caccttcccg
    85861 cacatagttc atcattgcct caaactgtgg gcgctgggta ttcttgcctg acgctttatc
    85921 ttcaaagact ttatcaagag ctactccagc taattgacgg tctgtgtttt gttgcacagt
    85981 cgatacccta atgtatccaa tattagccat tttctttatt ccttctgtgt tgatttgtgt
    86041 gtagtgatta ttgatcgtgt tgattaggtg tgtaaaggac ttttttcact acgaaattcg
    86101 aaggttttta gtgtggtgat ttagcctgct ttatcactac acgtcttgca tgagaatcat
    86161 gtagttacat tttggtaaaa ttagcaagct attaaatagt gtgtttattt tgaaaaaata
    86221 attttttgtt gcattaaagg aaacttgttc aataaatgat agaacgttta ttgagcaaca
    86281 attttgcagt gatgcgctat atgccgcatt acataaggct ttgctgatga taatgtaaaa
    86341 gtgaagaaag ctaacattgc tcttttaagc tacttttcat tgaattttta gtatggaatt
    86401 acatagggat aaactatgaa tattctatga tttacacgag atattcttgc tgtttaattc
    86461 cggctttgga gatcaggcat tcgcaagaat acgctaacgc gttgcctgaa aagccattgc
    86521 acataataga ggtgagcgta tagcgaacta tcaatgtgat cattgcacga atgataatga
    86581 gtggatatag attctatata tcgttagatt gtagtttgat agttaatgtg taatgtagat
    86641 tgtatgagcg tatagcgaat gttagttaga tcaacaacaa actaaccttt aaatctcttt
    86701 tatcttctca tagatatggc tatgcgtagc aaccgaaggt tagccaatca tctctatgca
    86761 gccgtaaggc tgttttgcat gtaatagttg tttgttgttg ttagacagtt accaatttat
    86821 agatcagaga tagatgtgag cgttgagcga tacactaatc ataacaacat cttcttactt
    86881 tttagttagt tgttagtagg ttattgttat gcgcgctatt agcgcatgta taaaggtatc
    86941 atttttatct aaagggcttt agacattttt tacacctttt tctgtgcaag ac
//
LOCUS       DAZFDP010000003        85731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFDP010000003 DAZFDP010000000
VERSION     DAZFDP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..161
                     /gene="yhfL"
                     /locus_tag="ACHJ1G_000225"
     CDS             <1..161
                     /gene="yhfL"
                     /locus_tag="ACHJ1G_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4762144.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
     gene            456..1793
                     /gene="frlA"
                     /locus_tag="ACHJ1G_000226"
     CDS             456..1793
                     /gene="frlA"
                     /locus_tag="ACHJ1G_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4762145.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1814..2836
                     /gene="frlB"
                     /locus_tag="ACHJ1G_000227"
     CDS             1814..2836
                     /gene="frlB"
                     /locus_tag="ACHJ1G_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4762146.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2886..3716
                     /gene="frlC"
                     /locus_tag="ACHJ1G_000228"
     CDS             2886..3716
                     /gene="frlC"
                     /locus_tag="ACHJ1G_000228"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4762147.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3713..4498
                     /gene="frlD"
                     /locus_tag="ACHJ1G_000229"
     CDS             3713..4498
                     /gene="frlD"
                     /locus_tag="ACHJ1G_000229"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO4762148.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4598..5329
                     /gene="frlR"
                     /locus_tag="ACHJ1G_000230"
                     /gene_synonym="yhfR"
     CDS             4598..5329
                     /gene="frlR"
                     /locus_tag="ACHJ1G_000230"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4762149.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5481..6566)
                     /gene="yhfS"
                     /locus_tag="ACHJ1G_000231"
     CDS             complement(5481..6566)
                     /gene="yhfS"
                     /locus_tag="ACHJ1G_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4762150.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6578..7882)
                     /gene="yhfT"
                     /locus_tag="ACHJ1G_000232"
     CDS             complement(6578..7882)
                     /gene="yhfT"
                     /locus_tag="ACHJ1G_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO4762151.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7894..8247)
                     /gene="yhfU"
                     /locus_tag="ACHJ1G_000233"
     CDS             complement(7894..8247)
                     /gene="yhfU"
                     /locus_tag="ACHJ1G_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO4762152.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8258..9136)
                     /gene="php"
                     /locus_tag="ACHJ1G_000234"
     CDS             complement(8258..9136)
                     /gene="php"
                     /locus_tag="ACHJ1G_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO4762153.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9133..10359)
                     /gene="yhfW"
                     /locus_tag="ACHJ1G_000235"
     CDS             complement(9133..10359)
                     /gene="yhfW"
                     /locus_tag="ACHJ1G_000235"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4762154.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10359..11522)
                     /gene="yhfX"
                     /locus_tag="ACHJ1G_000236"
     CDS             complement(10359..11522)
                     /gene="yhfX"
                     /locus_tag="ACHJ1G_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO4762155.1"
                     /translation="MFVEALKRQNPALISAVLSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11606..11968)
                     /gene="yhfY"
                     /locus_tag="ACHJ1G_000237"
     CDS             complement(11606..11968)
                     /gene="yhfY"
                     /locus_tag="ACHJ1G_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO4762156.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11985..12890)
                     /gene="yhfZ"
                     /locus_tag="ACHJ1G_000238"
     CDS             complement(11985..12890)
                     /gene="yhfZ"
                     /locus_tag="ACHJ1G_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO4762157.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13159..14163)
                     /gene="trpS"
                     /locus_tag="ACHJ1G_000239"
     CDS             complement(13159..14163)
                     /gene="trpS"
                     /locus_tag="ACHJ1G_000239"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4762158.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14156..14914)
                     /gene="gph"
                     /locus_tag="ACHJ1G_000240"
     CDS             complement(14156..14914)
                     /gene="gph"
                     /locus_tag="ACHJ1G_000240"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4762159.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14907..15584)
                     /gene="rpe"
                     /locus_tag="ACHJ1G_000241"
     CDS             complement(14907..15584)
                     /gene="rpe"
                     /locus_tag="ACHJ1G_000241"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4762160.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15602..16438)
                     /gene="dam"
                     /locus_tag="ACHJ1G_000242"
     CDS             complement(15602..16438)
                     /gene="dam"
                     /locus_tag="ACHJ1G_000242"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4762161.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16545..17831)
                     /gene="damX"
                     /locus_tag="ACHJ1G_000243"
     CDS             complement(16545..17831)
                     /gene="damX"
                     /locus_tag="ACHJ1G_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4762162.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17923..19011)
                     /gene="aroB"
                     /locus_tag="ACHJ1G_000244"
     CDS             complement(17923..19011)
                     /gene="aroB"
                     /locus_tag="ACHJ1G_000244"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4762163.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(19068..19589)
                     /gene="aroK"
                     /locus_tag="ACHJ1G_000245"
     CDS             complement(19068..19589)
                     /gene="aroK"
                     /locus_tag="ACHJ1G_000245"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4762164.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19990..21228)
                     /gene="hofQ"
                     /locus_tag="ACHJ1G_000246"
     CDS             complement(19990..21228)
                     /gene="hofQ"
                     /locus_tag="ACHJ1G_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4762165.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21140..21544)
                     /gene="hofP"
                     /locus_tag="ACHJ1G_000247"
     CDS             complement(21140..21544)
                     /gene="hofP"
                     /locus_tag="ACHJ1G_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4762166.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21534..21974)
                     /gene="hofO"
                     /locus_tag="ACHJ1G_000248"
     CDS             complement(21534..21974)
                     /gene="hofO"
                     /locus_tag="ACHJ1G_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4762167.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21958..22497)
                     /gene="hofN"
                     /locus_tag="ACHJ1G_000249"
     CDS             complement(21958..22497)
                     /gene="hofN"
                     /locus_tag="ACHJ1G_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4762168.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22497..23276)
                     /gene="hofM"
                     /locus_tag="ACHJ1G_000250"
     CDS             complement(22497..23276)
                     /gene="hofM"
                     /locus_tag="ACHJ1G_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4762169.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23396..25948
                     /gene="mrcA"
                     /locus_tag="ACHJ1G_000251"
     CDS             23396..25948
                     /gene="mrcA"
                     /locus_tag="ACHJ1G_000251"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4762170.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26114..26674)
                     /gene="nudE"
                     /locus_tag="ACHJ1G_000252"
     CDS             complement(26114..26674)
                     /gene="nudE"
                     /locus_tag="ACHJ1G_000252"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4762171.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26994..29129
                     /gene="igaA"
                     /locus_tag="ACHJ1G_000253"
     CDS             26994..29129
                     /gene="igaA"
                     /locus_tag="ACHJ1G_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4762172.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29194..29862
                     /gene="yrfG"
                     /locus_tag="ACHJ1G_000254"
     CDS             29194..29862
                     /gene="yrfG"
                     /locus_tag="ACHJ1G_000254"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4762173.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29873..30274
                     /gene="hslR"
                     /locus_tag="ACHJ1G_000255"
     CDS             29873..30274
                     /gene="hslR"
                     /locus_tag="ACHJ1G_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4762174.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30299..31177
                     /gene="hslO"
                     /locus_tag="ACHJ1G_000256"
     CDS             30299..31177
                     /gene="hslO"
                     /locus_tag="ACHJ1G_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4762175.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31312..33036)
                     /gene="yhgE"
                     /locus_tag="ACHJ1G_000257"
     CDS             complement(31312..33036)
                     /gene="yhgE"
                     /locus_tag="ACHJ1G_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4762176.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33415..35037
                     /gene="pckA"
                     /locus_tag="ACHJ1G_000258"
     CDS             33415..35037
                     /gene="pckA"
                     /locus_tag="ACHJ1G_000258"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4762177.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35113..36465)
                     /gene="envZ"
                     /locus_tag="ACHJ1G_000259"
     CDS             complement(35113..36465)
                     /gene="envZ"
                     /locus_tag="ACHJ1G_000259"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4762178.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36462..37181)
                     /gene="ompR"
                     /locus_tag="ACHJ1G_000260"
     CDS             complement(36462..37181)
                     /gene="ompR"
                     /locus_tag="ACHJ1G_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4762179.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37409..37885
                     /gene="greB"
                     /locus_tag="ACHJ1G_000261"
     CDS             37409..37885
                     /gene="greB"
                     /locus_tag="ACHJ1G_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4762180.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37982..40303
                     /gene="yhgF"
                     /locus_tag="ACHJ1G_000262"
     CDS             37982..40303
                     /gene="yhgF"
                     /locus_tag="ACHJ1G_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4762181.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40741..40968
                     /gene="feoA"
                     /locus_tag="ACHJ1G_000263"
     CDS             40741..40968
                     /gene="feoA"
                     /locus_tag="ACHJ1G_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4762182.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40985..43306
                     /gene="feoB"
                     /locus_tag="ACHJ1G_000264"
     CDS             40985..43306
                     /gene="feoB"
                     /locus_tag="ACHJ1G_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4762183.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43306..43542
                     /gene="feoC"
                     /locus_tag="ACHJ1G_000265"
     CDS             43306..43542
                     /gene="feoC"
                     /locus_tag="ACHJ1G_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4762184.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43745..44623
                     /gene="rpnA"
                     /locus_tag="ACHJ1G_000266"
     CDS             43745..44623
                     /gene="rpnA"
                     /locus_tag="ACHJ1G_000266"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4762185.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44652..45422)
                     /gene="bioH"
                     /locus_tag="ACHJ1G_000267"
     CDS             complement(44652..45422)
                     /gene="bioH"
                     /locus_tag="ACHJ1G_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4762186.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45460..46143
                     /gene="gntX"
                     /locus_tag="ACHJ1G_000268"
     CDS             45460..46143
                     /gene="gntX"
                     /locus_tag="ACHJ1G_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4762187.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46202..46777
                     /gene="nfuA"
                     /locus_tag="ACHJ1G_000269"
     CDS             46202..46777
                     /gene="nfuA"
                     /locus_tag="ACHJ1G_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4762188.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47138..48454
                     /gene="gntT"
                     /locus_tag="ACHJ1G_000270"
     CDS             47138..48454
                     /gene="gntT"
                     /locus_tag="ACHJ1G_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4762189.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKILADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48499..50583)
                     /gene="malQ"
                     /locus_tag="ACHJ1G_000271"
     CDS             complement(48499..50583)
                     /gene="malQ"
                     /locus_tag="ACHJ1G_000271"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4762190.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50593..52986)
                     /gene="malP"
                     /locus_tag="ACHJ1G_000272"
     CDS             complement(50593..52986)
                     /gene="malP"
                     /locus_tag="ACHJ1G_000272"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4762191.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53598..56303
                     /gene="malT"
                     /locus_tag="ACHJ1G_000273"
     CDS             53598..56303
                     /gene="malT"
                     /locus_tag="ACHJ1G_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4762192.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56346..57362)
                     /gene="rtcA"
                     /locus_tag="ACHJ1G_000274"
     CDS             complement(56346..57362)
                     /gene="rtcA"
                     /locus_tag="ACHJ1G_000274"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4762193.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57366..58592)
                     /gene="rtcB"
                     /locus_tag="ACHJ1G_000275"
     CDS             complement(57366..58592)
                     /gene="rtcB"
                     /locus_tag="ACHJ1G_000275"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4762194.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58781..60379
                     /gene="rtcR"
                     /locus_tag="ACHJ1G_000276"
     CDS             58781..60379
                     /gene="rtcR"
                     /locus_tag="ACHJ1G_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4762195.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60361..61119)
                     /locus_tag="ACHJ1G_000277"
     CDS             complement(60361..61119)
                     /locus_tag="ACHJ1G_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4762196.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61136..61966)
                     /gene="glpG"
                     /locus_tag="ACHJ1G_000278"
     CDS             complement(61136..61966)
                     /gene="glpG"
                     /locus_tag="ACHJ1G_000278"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4762197.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62011..62337)
                     /gene="glpE"
                     /locus_tag="ACHJ1G_000279"
     CDS             complement(62011..62337)
                     /gene="glpE"
                     /locus_tag="ACHJ1G_000279"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4762198.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62527..64032
                     /gene="glpD"
                     /locus_tag="ACHJ1G_000280"
     CDS             62527..64032
                     /gene="glpD"
                     /locus_tag="ACHJ1G_000280"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4762199.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64084..64689)
                     /locus_tag="ACHJ1G_000281"
     CDS             complement(64084..64689)
                     /locus_tag="ACHJ1G_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762200.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(64692..65459)
                     /locus_tag="ACHJ1G_000282"
     CDS             complement(64692..65459)
                     /locus_tag="ACHJ1G_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762201.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(65462..66172)
                     /locus_tag="ACHJ1G_000283"
     CDS             complement(65462..66172)
                     /locus_tag="ACHJ1G_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762202.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(66188..67696)
                     /locus_tag="ACHJ1G_000284"
     CDS             complement(66188..67696)
                     /locus_tag="ACHJ1G_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4762203.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(67822..70269)
                     /gene="glgP"
                     /locus_tag="ACHJ1G_000285"
     CDS             complement(67822..70269)
                     /gene="glgP"
                     /locus_tag="ACHJ1G_000285"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4762204.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(70288..71721)
                     /gene="glgA"
                     /locus_tag="ACHJ1G_000286"
     CDS             complement(70288..71721)
                     /gene="glgA"
                     /locus_tag="ACHJ1G_000286"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4762205.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71721..73016)
                     /gene="glgC"
                     /locus_tag="ACHJ1G_000287"
     CDS             complement(71721..73016)
                     /gene="glgC"
                     /locus_tag="ACHJ1G_000287"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4762206.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73034..75007)
                     /gene="glgX"
                     /locus_tag="ACHJ1G_000288"
     CDS             complement(73034..75007)
                     /gene="glgX"
                     /locus_tag="ACHJ1G_000288"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4762207.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75004..77190)
                     /gene="glgB"
                     /locus_tag="ACHJ1G_000289"
     CDS             complement(75004..77190)
                     /gene="glgB"
                     /locus_tag="ACHJ1G_000289"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4762208.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77463..78566)
                     /gene="asd"
                     /locus_tag="ACHJ1G_000290"
     CDS             complement(77463..78566)
                     /gene="asd"
                     /locus_tag="ACHJ1G_000290"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4762209.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78758..79351
                     /gene="yhgN"
                     /locus_tag="ACHJ1G_000291"
     CDS             78758..79351
                     /gene="yhgN"
                     /locus_tag="ACHJ1G_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4762210.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79408..80748)
                     /gene="gntU"
                     /locus_tag="ACHJ1G_000292"
     CDS             complement(79408..80748)
                     /gene="gntU"
                     /locus_tag="ACHJ1G_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4762211.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(80752..81279)
                     /gene="gntK"
                     /locus_tag="ACHJ1G_000293"
     CDS             complement(80752..81279)
                     /gene="gntK"
                     /locus_tag="ACHJ1G_000293"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4762212.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(81418..82413)
                     /gene="gntR"
                     /locus_tag="ACHJ1G_000294"
     CDS             complement(81418..82413)
                     /gene="gntR"
                     /locus_tag="ACHJ1G_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4762213.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(82637..83332)
                     /gene="yhhW"
                     /locus_tag="ACHJ1G_000295"
     CDS             complement(82637..83332)
                     /gene="yhhW"
                     /locus_tag="ACHJ1G_000295"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4762214.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(83455..84492)
                     /gene="yhhX"
                     /locus_tag="ACHJ1G_000296"
     CDS             complement(83455..84492)
                     /gene="yhhX"
                     /locus_tag="ACHJ1G_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4762215.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            84825..85313
                     /gene="yhhY"
                     /locus_tag="ACHJ1G_000297"
     CDS             84825..85313
                     /gene="yhhY"
                     /locus_tag="ACHJ1G_000297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4762216.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            85550..>85731
                     /locus_tag="ACHJ1G_000298"
     CDS             85550..>85731
                     /locus_tag="ACHJ1G_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO4762217.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGIT"
BASE COUNT        20683 a        23379 c        21539 g        20130 t
ORIGIN      
        1 aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc
       61 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta
      121 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
      181 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
      241 tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttga taactacatc
      301 cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat tacataacat
      361 catatgttgt tatattcatc atgcattgtc atgttacctt ttaaatgact gcaaactctc
      421 ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaggaa ctccaacgca
      481 agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt
      541 ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt
      601 ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg
      661 cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg
      721 ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg
      781 cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat
      841 ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg
      901 gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg
      961 gccttgggat cttctggttt aaagcggaga attttgccgc ccctaccacc actgcgattg
     1021 gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata
     1081 ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc
     1141 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
     1201 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
     1261 ccctgacctg gatccccgca ctcggcagca ccgctgggat ctttgttgcc atcacggcga
     1321 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
     1381 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca
     1441 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
     1501 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
     1561 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
     1621 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
     1681 ttgtctgggc accgattccc ggccttatct gcgccgtcat cgttattgct actggtctgc
     1741 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
     1801 tggagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
     1861 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acaccatcgt
     1921 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gatcgccact
     1981 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
     2041 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
     2101 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
     2161 ggcctgcggc gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
     2221 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
     2281 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
     2341 caaaaacgat ctcaagcagt tgccgaatgc gctcggtcaa ctggtacgca cctgggaaga
     2401 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
     2461 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
     2521 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
     2581 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
     2641 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
     2701 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
     2761 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
     2821 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccgaatttac gcaaggagtt
     2881 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
     2941 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
     3001 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
     3061 atcagatgcc gattatcggc tatacgccag aaaccaacgg ctatccgtat aacatgatgc
     3121 tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat caagctggcg atggatatgg
     3181 caaaagagat gaacgcgggt tatacgctga tttccgcggc ccacgcgggc tatctcacgc
     3241 cacctaatgt tatctgggga cggctggcag agaacctaag cgaactgtgt gagtacgcgg
     3301 aaaacatcgg catggatcta atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
     3361 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
     3421 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
     3481 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
     3541 acattcctgg agaaggcaaa atgccgctga gggaactgat gcgcgatatt atcgaccggg
     3601 gctatgaggg ttactgtacg gtggagctgg tgacgatgta tatgaacgag cccagactct
     3661 atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac
     3721 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
     3781 tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg
     3841 cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat
     3901 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
     3961 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
     4021 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
     4081 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
     4141 cttctccgac aagtgggaca gctcgctctg gcagacactg gtgccgcatc tcgattttgc
     4201 ctttgcctcc gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
     4261 ccgtggcgca ggcgtggtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg
     4321 cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg
     4381 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
     4441 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
     4501 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
     4561 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
     4621 tcatcaactc ctctacgcta ccgtccgcca gcggttgctg gatgatatcg cgcagggggt
     4681 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacgcaat ataacgtcag
     4741 ccgcattact attcgcaaag ctatcagcga cttagtggca gacggcgtac tgatccgctg
     4801 gcagggcaaa ggcacctttg tacaaagcca gaaggtcgaa aacgccctgc tgaccgtcag
     4861 tggttttacc gattttggcg tctcacaagg caaggcgacg aaagagaaag tgatcgagca
     4921 ggaacgagtc agcgccgcgc cgttttgcga aaagctgaac atccctggca acagcgaagt
     4981 gttccatctt tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcctg
     5041 gatcccactg tcgcgttatc ctgactttga tgagatttac gttgaaggaa gctccactta
     5101 tcagttattt caggagcgtt tcgacacgcg agtggtcagc gacaaaaaga ccatcgatat
     5161 ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt
     5221 tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt
     5281 ctgccgcgcc aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg
     5341 ataagatgcg ccagcatcgc atccggcgat gctggcgcgt tgaattttac atcccgtacg
     5401 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcagta cggaatttag
     5461 cttgacggtg ctatccagca ttaaatactg gcaatactct cacgcaaaat ccgcagcacc
     5521 gtctcttcac cgctgcgatt cgggttaatg cgaatcgcac aatgttctga ttgtggattc
     5581 gcctggcgaa acgttccgga aaggcgataa aagagcggcg ggatttcata tttcgactct
     5641 gcacccaccg ggtaaggcaa ggcaccgcgc ttttgcgcct cttccagcac tctggcggca
     5701 atcggctgat gaaactcgac aatcaacacc ttcgactgcg cattagcaat caccgcgctt
     5761 ttcacttccg gcaccgcacc accgttaagc aaagccagca accgttcaga tacccctgcc
     5821 tgcaccgcgt gcatcactgg cgcaaacacc agaccacgca atacttccag cgcctgtgcg
     5881 ccctggatct ggctaccgcc ggagtaaagc gtggcgcgaa tacggttgat aacatcagca
     5941 tcgccgacca ctgcaccaac accctctggc ccaaatagct tgaagcagga aaatgtcgag
     6001 acattcgcgc cgcattcaca gccgattcgc gccaccttca tcaccgcata gttgtcatcg
     6061 gttaacgctg gaacacctgc cgcgcgcaac gttgccagca catctgccag cacgtagctg
     6121 tcctgcggct gctggcgcgt atgctgcacc agcgccgcat ccggttgttg ctcgtcgacg
     6181 acctgcttca gtgccgacag gtcattgaaa tcaacagtaa taagcgtcag ccccatctgc
     6241 tcaataataa cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc
     6301 ggtttgagca aagccgccag cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc
     6361 gccgcagcct gtgcgtgaaa tgcatcagcc agcacctgct ccacccgttg ggtaatacgc
     6421 ggttgattca gacctggagt taaccctaaa tcaccgccag taagaaactc gctgccgggg
     6481 aaatggcgac aaatgctatc caccagcgca aactgctttt gctgcgcctc aataatcgtc
     6541 aggctttgca gaggaaacgt cttcataggt gccttcctta agccggaacg aacaggccaa
     6601 gccagtaaag aacattcagc agaataccgg tgatcattac tgccacgacc ggtgccgcca
     6661 ttttctgtac cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga
     6721 atccggtata acccgccatc ttaattgctg cgaaaatcga accgaccagc agcgccactt
     6781 ccatcagcat attcatggcg ttacggatgt tatccgacgc attacgcacc gacgggtagc
     6841 gtcccagcca tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc
     6901 ctaataccgc cgcaaccatc ggatttggcg agagatagcc caccgcataa acaaaggtaa
     6961 agcccgcaac tgcatacacg ccagtggcta acgcggtggt ggcaatcaac ggcacaaaac
     7021 ccagtccgcg cataaattct gccagagccg cctgattaat cagcgtttgc gattgttccg
     7081 gcgtcacgcc tgcggaatac gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa
     7141 aaatcttcat actggcaacg gcggcaatca atgctcccac gatggcgata taaggtaagt
     7201 ttttgataat ccgcgacgtg cgctcttcaa acaccgaaag cccgctggca tcgatgtcat
     7261 tttcatcacg atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc accatgccaa
     7321 taaagatttc gatggattca gggttaagat gtgggaaata gcgcacgact accacacggg
     7381 tcatcagaac aaccacggcg gcgaccagac tttgcttcca gccaaactgg tagaaaatcg
     7441 ccaccagcgg gaacagtgca aaagcagaaa ccaccggcga gcttaattcc ccgaggctac
     7501 ctaatacatc caccggcagc gcggtcagca gctggtttac tggcaacagg caagtaagga
     7561 tcaacacgcc ccagatagcg ccaagaccaa acgccatcag gctgtttatc gccagtacgc
     7621 cgaggatatc ggtaggaaga aagagtaacc atgcgttgag cagcccggtt ttcagggtaa
     7681 aagagatccc caccgaggcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct
     7741 cgcgacgatt catatagcct tcaatcagtt gcggcaggat cgggcggatg ccgtcatgaa
     7801 aaacagccgc cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac gccaccacga
     7861 taatctgaat atacagatcc atagtcgtgc cttttatttc agatggttaa tcagcatcgg
     7921 aatcgcgtgt tcgacgtgct caacggaaag gccaaacgcc actttccctt cagcgatcat
     7981 tttggcgata tgctcgtctt tcgctttaat gcctggtttg gcaatggtgc aacttttgtt
     8041 atagccaatc accgcgatgg caatcgacaa tgccgcgccc gcaccggtat tacacgcacc
     8101 gatgtagtaa tccagttgcc cggatttcac tttcattgcc gcttccatgt cgttgtgaat
     8161 gaaaacttca aaactgccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg
     8221 taagcctgca acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat
     8281 tttcacgtaa catcacatcc acatcggcct gactgaatcc tgactggcgc aattgcggaa
     8341 taaaggtggt caataaatag tcatagccat aaccaccatt ggcttttaaa tgggagcggc
     8401 gcgtaatatc catcgacagc atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga
     8461 gcatcgcaat gcgcttttcg tccgggtagt aactgttctt accaatggtg tcgaactgca
     8521 cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag attgtctttc agatcgcagt
     8581 gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgttcca
     8641 gccccatggt gctgaacgaa gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca
     8701 gcgcagcggc aataaacacc ttctcttcca gcggcgtaat cttcccttcg ctagagccga
     8761 tctccgcgat aatcccggct ttcaggtcgg tgccatcgat accctgttca atttcatcga
     8821 ccatctcctg cgccagttcc tgcacgctgc gggtcgccac atgctccggg aaaaaagcgt
     8881 cctggtaata accggtgcag gccaccacgt tgatccccgt ctcgcgcatt acatcgagca
     8941 taaattgcgc attgcgcccc atgtaacggt tggtcatctc aatcacatta cgcacgcccc
     9001 gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca
     9061 cgttgttttt aaagccggag agatcaatat gcagatgctc atgggccagg gtgtaacccg
     9121 tcggatcaat actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc
     9181 attttgtggc ggtggcgcac ggaaaaattc acacacggta gcccccacat cagaaagcgt
     9241 ggtgcgtaca ccgagttgcg tgtggaccag cccttgctga taaaccagca ctggcaccac
     9301 ttcacgggta tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac
     9361 caggcaatca tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg
     9421 caaacgttcg gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat
     9481 attgatgcaa ataaacgccg tcggatgcgt gttaaattcg tcgagggtga tatccataat
     9541 ccgctggcta tccaccaggt tttgccagct cacgccataa ggattgctga cgatatctgc
     9601 caccttacct accagcacgg ttggcacgcc cgcctcatac agtttttgcg gcacctgcac
     9661 tttttcatca acaccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga
     9721 acgcggcgca ttaataccaa taaagcgccc ttctttgctt tctgcggcat cgagaatacg
     9781 ctgactgtcg gttaacaagc cgccaaatgt aatgacccga ccgacctgta cctgctcacg
     9841 cacgatacga ccaattttga tcgcgtcgtc aaaagagatc acagagagat tggcggtaat
     9901 gttatagacc tggcctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac
     9961 ccacagaaat tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc
    10021 ctgctcaaca cggccaatca catcgcgaaa gggcatccgc agcggcggta acgggcgcgt
    10081 gcctaaaatt tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac
    10141 cccccaggtt gcggaatctg acggttgcat atcacctggt gcataaccca atgcgttgat
    10201 tagccccagc ttctccagcg ctggtagctg caaatgcggc aactggctca ggatgtgacc
    10261 acaggtattc gccccggcat cttgcgggcg caccagcgtg acatctttca ttgcccctac
    10321 gccaaagcta tcaatcacta acaccacaaa tcgcgccatt acgcccccag agagtttcca
    10381 agactgtcat aacgaccgac gattttcggt tcgccacggt gaataccgga caccagcacc
    10441 acatcgctac gagtgacaaa aatctgcgtg cgaaaacaga gcactactgc actgcttacc
    10501 ggaaactcgc ctgccagcgg cagggtgtag tcgatactgc tgtcatccac agttttgaga
    10561 ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg cgcatgacca
    10621 cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg ggagatttca
    10681 cttaaccaaa gcatcgctat atattcaggc tgatcgccct gctggtttgc cggaatagtg
    10741 cccgtcagcg catgaccggg ttcggcatga gtcacaccgt attccgccag catcggcagc
    10801 gaagtgcagc tggtcgcaga aggcgcgttc agttgctcaa tcgcaatacc agatttcgcc
    10861 agttgatccc gtgcttgtac cagcgtgtga agattcggtg tcggcaaaac tttcccggca
    10921 gcctcatccc aaagcaggca agggaaatgg gtaagtccgg ctaaatgcag acccggcagt
    10981 ttcttaattt cagcaaccac ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc
    11041 ggataaagaa aatcatcgtc gctataaact ttgagcagca caaactgcac acgtccggcc
    11101 ttcaccgctg ccgcagaaac ttcccgcgct ttgtcgagag taaacacggt gatgacgtcg
    11161 gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag atgcccctga
    11221 tgcgccaccg acaaaccagc gcggcgcatg actcgcgcct ctttgtaatc caccgccaca
    11281 atgccgctgt aacctaatgc cagcaatttt tccgccagcc atgggttacg gccaaactgc
    11341 ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatcag tcgcttaccg
    11401 ttttccagta cctgatccac gtcgatcacc cagctgtccg gggcgatctt gccctgctgc
    11461 cacaaactta gtacggcgga aatcagcgcc gggttctggc gtttcaatgc ttctacaaac
    11521 atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttgaa tatttaggtt
    11581 gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc cataatccat
    11641 aaaggttggc cagcaaatag ccttcttcac acgggttaac ttccagtgcg aattccttca
    11701 acaacacctg atgcaattgc acaacggctt gccagtggct ggattgcgcc agctctgcca
    11761 gcaattcgtt atccagcggc tctatttctt caccacggcg actgcgcatc agtgcactcg
    11821 ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg cactctgttt
    11881 ccagtacgtt gacgacctgc atcatgcctt tgcagatgtc cttatcaata acgcctgcct
    11941 cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata actcggttcc
    12001 tgttccccac tcactacccg ttgttgatgg gcgagcaggg cgtgtttatc tacaacggca
    12061 cgcagcagtt gttgcattgg gtaatcatcg actcgcgtca gaaccacggc ttcggtggcc
    12121 tgtaaaaatc gcggatcgtc tgtgagcggc gtcgcctcta atcccagcat ggtcagttcg
    12181 ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat gcgttgtaaa
    12241 ctctcgtgat aagagagatc gacacgttcc acatcactat cgccaaaaaa gacatcggtc
    12301 atgattttct gatccgccga acggttatcc agccccacgc gcttcacgtt tgcggactcg
    12361 cctttacggc aaatcaactg gtgctcgcca acgtaggtgt gcggccccaa ctccagcgcg
    12421 aggcaaaacc ctttttgcgt gagataactt tccgccgcca gtcgggaaac caccgccatg
    12481 tcatacacgc cgttaagtaa acactccacg cgaatatccg cgccgcgcat atgcgcatag
    12541 taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc gtacaagcgg
    12601 gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact cagcaatgct
    12661 ttgttatcca tctcgaccag ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct
    12721 ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caacggatga acggcattca
    12781 gttgccagct catctatggt tttcaaccta ttaccgcatt tttcacccaa caaataacgg
    12841 gccagcgtcg tgataacgac gccttctttt ttgataaacg ttcgacgcat aatatatttt
    12901 caataaaatg aatgtttata tcttcattaa aatgaagata agtggcaact gtgagatgtg
    12961 gaaacggcga ggcgcttcac attttttcgg attattgcaa atgaggataa aaaaaccggg
    13021 tttccccggt ttcagagtga tgacgaagtg atttattcga caatgcgagc aaagggtaat
    13081 tcccactggc gcttgcctgc tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
    13141 cgtccgagcg ttttgatatt acggcttcgc cacaaaacca atcgcttcgt acaccgcttt
    13201 cagcgtacgg gaagcgtgta cactggcttt ttctgcgccg tctttcatca cctgttgcag
    13261 gaaggcttca tcgttgcgga aacggtgata gcgttcctgc aattcagtca gcataccgga
    13321 aacggcatca gccacttcac ctttcagatg accatacatc ttgccttcga actgtttttc
    13381 cagttctgga atgctctggc ccgttaccgc agaaaggata tccagcaggt tggaaacgcc
    13441 cgctttgttc tgcacatcgt agcgaactac cggcggctcg tcggagtcag tgaccgcgcg
    13501 tttgattttc ttcactaccg atttcggatc ttccagcagg ccgataacgt tattgcggtt
    13561 atcgtcagac ttggacatct tcttggtcgg ctccagcagc gacattacgc gcgcgccaga
    13621 tttcggaata aacggctccg gcaccttaaa gatctcgcca tacagcgcgt tgaaacgctg
    13681 ggcgatatcg cgactcagtt ccaggtgctg tttctggtct tcacccaccg gtaccagatt
    13741 agtttgatac agcaggatgt ccgcagccat cagcaccgga tagtcaaaca gaccagcgtt
    13801 gatgttctcg gcataacgcg cagatttatc tttaaactgc gtcatgcggc tcagttcgcc
    13861 gaagtaggta tagcagttca gtgcccagcc taactgcgca tgttctggta cgtgggactg
    13921 aacaaaaatg gtgcttttct caggatcgat accacaagcc agatacaagg ccagcgtatc
    13981 caacgtcgct ttacgcagct tctgtgcatc ctggcgcacg gtgatcgcgt gttggtcaac
    14041 gatacagtaa atgcaatggt agtcatcctg catgtttacc cactgacgca gcgcacccat
    14101 gtagttacca atggtcaatt cacctgaggg ctgtgcgcca ctaaaaacga tgggcttagt
    14161 catttttcga ttcctgattt tcgctatgcg gaagccctaa tgcgggcaga aggtcattta
    14221 tagactgata aattacatca ggctggctga gatcgatagc ctcgccgtag ttatatccgt
    14281 aggttaagcc aactgatggg caacctgccg cttttgccgc ctgaatatca ttgcgtgagt
    14341 cgccgacaaa cagcatctgt tgtggggcaa ttcccatccg ctcagccacc agtaacagcg
    14401 ggtccggatg cggttttttg ttttgcacat catcgccgcc gataaccacg ctaaagtatt
    14461 tggcaatatc taaggcttcg agcagcggcg cgacgaacgg cgtcggtttg ttggtgacca
    14521 ggcctagcgg caggccttta gcctgcaacg cgcccaacgt atcggcaacg tgcgggaaca
    14581 aaaacgtccc ctcttcggca acctcgccat agtagcgatc gaacagttta cgcagaatac
    14641 gtacctgttc ttctgccgga atgtcgtcat caacgggcgg tttacccatt gttttacgct
    14701 gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc catcagaaca tctgcgccgt
    14761 taccaatcca ggtaataacg cgttcttcac ctgcgacggg caactccagc gcatacagcg
    14821 ccatatctac cgcagcagca agaccgggag cactgtcgac cagcgtacca tcaagatcaa
    14881 aagcgacgcc gcgaatatct tcaaacttat tcatgactta cctttgccag ttcactgcgc
    14941 atttcatcaa tgactttttt gtagtctggc tggtcgaaga ttgccgaacc ggcgacgaac
    15001 atatccgcgc ccgccgcagc gatttcgcca atgttgttca ccttcacgcc accgtctact
    15061 tccagacgaa tgtcaaagcc agactcgtcg atacgacgac gtacttcgcg cagtttatcc
    15121 agtgtttgag gaatgaacga ctgaccgccg aaaccaggat tgacggacat cagcaggatc
    15181 acatccagct tatccatcac gtaatccaga tagctcagcg gtgtcgccgg gttaaatacc
    15241 agtcccgctt tacagccatt ttctttaatc agttgcagcg tgcggtcaac atgctcggag
    15301 gcttctggat gaaaggtaat gatgctggca ccagcggcag cgaaatcagg cacaatgcga
    15361 tcgacaggtt tcaccatcag gtgtacgtcg ataggggcgg taatgccgta gttacgcaag
    15421 gatttcagca ccattggccc aatcgtcaga ttgggaacat agtggttatc catgacgtca
    15481 aaatgcacga catcagcgcc agctgccagg gcttttgcgg tatcttcacc caggcgggca
    15541 aaatcagccg acagaattga gggggcaatc aaatactgtt tcatccgctt ctccttgaga
    15601 attatttttt cgcgggtgaa acgactcctg gtttgtacaa agccagcagt tcgtccacct
    15661 ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac tttgacgaca tgcaattttg
    15721 cgcgctgata ccactcacgc gttaacatcg tatcgtgatt ggagatcagc actggaatat
    15781 ggcgctcaac cagaccttcg gcgatctccg ccagatgcgc ttgttgttca agcgtaaaac
    15841 tgtttgtgtg atacgccgta aagttggcgg tcgcagacag cggtgcataa ggcggatcgc
    15901 aatagacgac ggatgcatca tctgcgcgcg ccatgctatc ggcgtaagac tcacaataga
    15961 aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc tgcttccggg aaatagggtt
    16021 ttttgtagcg gccaaacggc acgttaaact caccgcgcag attgtaacga cacaggccgt
    16081 tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg acggaacgga tcctggcttt
    16141 tgttgaactc ttcgcggaac tgatagtaga cctcggcgca atttgtttcg ggaacaaaca
    16201 gctcgcgtgc ggcctgtacg tactcatcag tacgcatctt cacaatgtta tagagactga
    16261 tcaggtcgct attgatatcg gcaaggatat aacgagaaaa gtcggtgttg agaaacaccg
    16321 acccggcacc tacaaaaggc tcaaccagac attcgccctt gggcaaatgc cgtttaatat
    16381 catcaagcag gggatacttg ccccctgccc acttcaaaaa agcgcgattt ttcttcatgc
    16441 tgactaacta attacacctt ctccggctgt ggagaaagct ccgacagcat ccagcgcttc
    16501 aggcaactcc cgcaacgata acccattgcg ggagataacc ttgattactt cagatcggcc
    16561 tgtacctgac gcagcggttt cgcccacggg tttttggcct gaacatctgc tggcaatgta
    16621 gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc cagaaaccag gacataccac
    16681 ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt tcagattctc tttcttcgcc
    16741 caaccgttca ggttgtcgta gttagaggaa ctgctcagct gcagagtgta atggctggac
    16801 ggtgccgatt tcaacgaacc aacattacct gcggtcttcc ctccagcggc tggtgttgcc
    16861 gtggtttgcg ccggggatgc cgtctgtact ggagccgtag tcgccgtctc ttttggtgct
    16921 ggcgcagaag tcgcagccgg tgccttcgtg ctcgccacag gagcagctgg ttcagtacgc
    16981 ttcggcgtct gtgctaccgg cttcggttcc gttttcacgg ttgcttgcgg ttttttcggt
    17041 tcaatcaccg cctgctgacg agctggacgc gtagtggccg gacgttcagc agtttgcgtt
    17101 ttcgccgtgt cacgcgatgc attgccattg cgtactggcg cgacagttgc tggttcagtc
    17161 ggcaatgtag aattgaccgc cacattgttc aactgttgct gattttgtgg ctgggtcagc
    17221 gcattgttca ggtcaccctg cacttcaaca cgttgttgac catccgttgc cgccggggtt
    17281 tgcccttgag tcggcgtaga agagatcggt ggcagagaaa catcctgctg agtattttcc
    17341 gcagacgtgg ttcccggcgc tggctgcacc ccattcgcct gatcggtcgc attgcctgca
    17401 agatcaatac tcttctcgcc agacgcggtt tgatcgctgg aagaggtcga gggggctttt
    17461 agcgcagaac cgataccgat gatcaacagc agtagaacca gaatgccgac gcccatcatc
    17521 atatactgac gagaagcggg tttactggct gcttttttgc gcttacgcgg acgacgctct
    17581 acgcgctctt catccacggt ttcatcttcg gattcgtcaa tttcttcttc gatttccggt
    17641 tcctcattgc gctctttttg cgcacgagtc ggacggcgat cgtcagtgtc atcaagttca
    17701 atatcatcaa aattgatctg cggttcgcca cgttcagtac gctcagaacg ttcagaagat
    17761 tgacgagaac gaccagtacg acgatcgctg ggatcgggtt tcagctcgtc ttctggtttg
    17821 aattcatcca tttaacaccc cactaaaagg ttaatgctta ccacattgca attaacctga
    17881 agctaataga ccgcttgata agcggcctga cctttcttgt tgttacgctg attgactatc
    17941 ggcaatggcg ttaagaacaa gctcgtgcga aacgccgctg cgaacttcac tcttaccaat
    18001 tgccaacgga agaattaagc gtatctctcc cgcaaggact ttcttgtcac gcagcatatg
    18061 cggtaaatac gcctgcgcgg acatttcgcg cggcccattg accggtaacc cagcccgctt
    18121 gagcagggtt ataatacgct gcgtttcggc agaactaaac tgcccgagac gttccgacgt
    18181 ccgcgccgcc atcaccatac ccgcagcgac cgcttcacca tgtaaccaat tgccataccc
    18241 catttcagct tcaatggcat gaccaaaggt gtgtcccaga ttcagtaaag cacgtaaccc
    18301 ggtttcgcgc tcgtcggcgg cgacaacttc tgccttcagt tcacaacaac ggcgaataca
    18361 gtacgccatt gccggaccgt ccagacgcaa caacgcatcc agattctctt ccagccagtt
    18421 aaaaaacgca ccgtcaagaa taatgccgta tttgatgact tctgccagcc ccgacgctaa
    18481 ctcacgcggg ggaagcgttt tcagacagtc gagatccacc accactgaag caggctggta
    18541 gaacgcgcca atcatgtttt taccgagggg atggttgacc gcagttttgc cgccaacgga
    18601 ggaatcgacc tgcgacagta acgtcgtcgg gacttgaatg aaacgaacac cgcgctgata
    18661 actcgccgcc gcgaagccgg tcagatcgcc cactacgccg ccgccaagcg ccaccagcgt
    18721 agtatcgcga ccatgcggct tttgtaacaa cgccgtaaag acggtatcga gtacagccag
    18781 gcttttatac tgctcgccgt cagggaggat aacgctatcg acgttaacac ccgcctgttc
    18841 aagtacgccg cggaccttat cgagatacag aggagccagg gtttcgttgg tgaccaacat
    18901 gacctgctcg cccgatttca gcggtaagaa tgaagctggt tcattaaaca aaccagatgc
    18961 gatggtaatt gggtaactac gttccccgag agtaacgaca atcctctcca taacgcgaca
    19021 tccaccttaa ttactgtacc cgcagacgag tgtatataaa gccagaatta gttgctttcc
    19081 agcatgtgaa taatctggtt tgcaaccact ttagcgcttt gatcatcagt acgaatggtc
    19141 acgtcggcaa tctcttcata cagcggattg cgttcattgg ccaacgcttc cagaacttca
    19201 cgcggcggtg tttcaacgtg cagcaacggg cgttttttat cacgctgcgt gcgtgcaagt
    19261 tgcttttcga tggtcgtttc aagataaacg acaacgccac gagcggaaag acggttacgc
    19321 gtttcacggg atttcacaga gccgccgcca gtagccagca caataccctg tttctcggtc
    19381 aactcattga tgaccttttc ttcgcgatcg cggaagcctt cttcgccttc taaatcgaaa
    19441 acccagccca catcagctcc ggttcgtttc tcaatctctt gatcggaatc gtaaaattcc
    19501 atattgagtt gttgagctaa ctggcgccca atagtgcttt ttccggcacc cataggccca
    19561 accagaaaga tattgcgttt ctctgccatt ttttcggtac tactaagact attcgttaat
    19621 gataaacccg cttcgctcag agagcgccgc aggacatgaa ctgaaacctc ataagatatt
    19681 gcgagagtca gactgaaaat tatctcaata ctccagcggg tttggcaact gaataaatca
    19741 cccatccctc tgcatatctg gtcgctgcaa gcgcgctgcc ttgctaccac cgctctggcg
    19801 ataaatcacc gggtaagatt agcgtaaaaa agacagcaaa atgccgcctg aatgataaat
    19861 catcatcatg gaacgcaggc agcgaagagc accaactctc aaatcggtac tccttgtatg
    19921 ctaaatacct gcgcgcgtca aatagatgaa acacgttcag cataaaaaca ttaccgcttt
    19981 tacggctgtt tactcactgg aaaccagtcg tggcgtgata aacaccacta actcgcgtcg
    20041 ttcatcttct tttccgtcat gacgaaataa ttgcccgaac cagggaatgt cgccaagcaa
    20101 cggtacgcta tcctgacccg atttattttt acgggtaaaa atgccgccca gcgccaacgt
    20161 ttctccgctt ttgacctcga cctgcgtttc gatctcctgc ttatcaatcg ccagcacttc
    20221 gccatcggcc tgctgtagca cctgccccgg aacgttctgg ctgatgtgta atttcagccg
    20281 gatgcgacct ttttgtaaca ccgtgggcgt gacctccatc cccaggacgg cctctttaaa
    20341 ttccaccgac gtcgcgccac tttccccgct ggaaacctga tatggaattt cgctcccctg
    20401 tttaatgctg gcaggctgaa gatgtgaggc cagcagacgc ggactggcga taatatccag
    20461 ctgctgtttt tgttcgagcg cagaaagctc aagatccagc aagcgtccgt tgatgcgccc
    20521 aatgttaaaa ccgacatgcg ttgtcgccgt cgctacggag aggtcgctac caagcgtggt
    20581 gacttgccca acgccaccag cgtgttgcgc atcggccagc gtccatttca cgcctaactc
    20641 acgcaaactt ttttcattaa tggtgacaat atgcgccgac agctcaacct gcccgaccgg
    20701 cagatccatt tgcgctaccc actgttcaag cgcgcttaac gccgttttgt tatctcgtag
    20761 caaaaggcga ttggtgcgtt tatcgacggt catactccct ttggcactca gtagcttctc
    20821 ccccgctttc gccagttctc ccgcgtcggc gtattgcagg gttatactgc gattttccag
    20881 cggcagattt gcctgcgccc gcgcctgctc cgcctcctgg cgggcgatat tgttattctg
    20941 ccaggcaatg gaatgcactg agagaatgtt gtcttcctgc cgcgttatca gtccggcgct
    21001 tttcactaca gtttgtagtg cctgcttcca gggaacatct gttagatgta acgacaccgt
    21061 accgctgacg tctggcgaca cgaccaggtt caacttctcc tgttcagcca gcgcctgcaa
    21121 cacctgagct accggaacgt catccaccat cagcgtcact ttttgcggct ttgccgcctg
    21181 tacgccgggt atcagcatca acagtagtgc ggctatccat tgcttcattt gtatctcctt
    21241 gccgttgcca caaccattgt ggcggttcgc agtttgtccc ggttcccagc gttagtgcgt
    21301 ctggcgtcag ctgtaaaatt gtccagccgt tttccagcac atcgttttgc tgcacccgtc
    21361 gccatttctt ttgcccgtct tttattacac cgatgatgtg ctcgcctcgc cctaccatcc
    21421 cctgatagcg ccactggcta agttcgctaa tccggcatag atcttccggc ggtttaaaag
    21481 ggtcacgcat accggttaaa aggcacaatg caatacctgc caacaaccag cgtttaaccc
    21541 tcatgcggtg tctccagttg tagcgtgaac aaaagatcat caccttccac gcttaacgaa
    21601 aaacggctca cgctgacgtt gcgctctgcc agccgtgtaa atgccgacgg cactgcttcc
    21661 cacagcgttt tcaacgccaa ctcacctccc tgcgcggatg gatgccagga aaccagttgc
    21721 gcgccgggca actgaaaatc cagtggcgaa aagggcagcg ttttttcttc gctgaaggga
    21781 gtggtgtcaa ccaggcgata cagtgtggcc cactgctggt gatgacttgc ccgtagtcga
    21841 attaatgcgt cgcgctcttc atggtgtgtc gatgacagaa aaatgagcgt aactaacatc
    21901 agcaaccaga atgcccagca aaactggcgg aggcggggtg atgtggcgaa ccaccagtca
    21961 aagaacatgt tcatcgctaa ccttccttgt taactgatac tcaaattgcc agcgtccctg
    22021 cgcatcctgc tgcgtggctc cccgctgatt gagatgaaaa gacgcatcct ggcggagtga
    22081 cgtttctagt gcgtttaacg cggtaatgct tgttgtcagc cccttgatct ccagcgttcc
    22141 ctgctgccag cttatcgttg tcagccaggc gtgctcgggt aaaagcgccg ccagtgcttc
    22201 cagcgcagat tgccagtcgc gggtaaattg tcgctggcgc tgccgttgcg aacgctgttc
    22261 gcgtaactgc tgttgctcca gcaaacgtgg cttcgttatc tgtaagctgc gggcgagttg
    22321 ttgttccgcc tgaagcaaaa ctgcgtctat gcgcgcttcg gcgctgcctg tcagacgcag
    22381 tattagcgtt atcccgacgg ccagcagcag aggcgcaacg aacatcagca accagaaacg
    22441 cagaaaagcg gtccggcgtt gctgtcgcca gggcaaaaaa ttaattggcg ggttcatcag
    22501 tacgccttcc caagcgccag ccccagcgcg atggcaaagt ctccaccagg cggcggtagc
    22561 ggcggctgac gaacagaaac ggcctcccag gggtcaaatc cgccttcgcc acatatcgcg
    22621 acgctgtcgg gatcaacgga taacgctgcc gccagctcct tcgcgctagt catccccacc
    22681 gccagtttgc gcccccagct atagcgcgtc gcccacagcc actgttcgtt atcacgccag
    22741 gccagacatt gctgatgaga aggtaaaaaa ggcaggaatc gctgtaatgc actggcatcc
    22801 ggggtgatcg cactaacatg aacacgcaac ctttctgcca gagtaagcag cgttgccagc
    22861 tctttgcttt gcgcggcagt cacgttatag gcgggactga gtgagtcttc gctgtaatcg
    22921 aaacgcaggg agtccggatc catatccagc tcgcgggcca tcgtccctga cagccaggcc
    22981 gtttgctccc gctcaccaag ggacatcgac gggcgcggaa atgaccgctg taatgtgcga
    23041 ctggcgggaa acgccaacat aatgtgatga cgctgcggca gttcgcgact ccacggtaac
    23101 aacgttttag ccagctgctg cgcatcaaca atccgcccat ctttgataat gtcgttctcc
    23161 agcggcaacc gccaccagcg ttgcaaaaag cattcttttg ctccccgcac gatcgcaacc
    23221 gctaccgctt cttgctgttg taaatgcaaa ccaatttgcc agatcttaaa tgccattgtg
    23281 atgctctcct tatcacccgt cactctgacg ggtatatcaa tgcgtctggc ttgcctttat
    23341 actaccgcgc gtttgtttat aaactgccca aatgaaacta aatgggaaat ttccagtgaa
    23401 gttcgtaaag tattttttga tccttgcagt ctgttgcatt ctgctgggag caggctcgat
    23461 ttatggccta taccgctaca tcgagccaca actgccggat gtggcgacat taaaagatgt
    23521 tcgcctgcaa attccgatgc agatttacag cgccgatggc gagctgattg ctcaatacgg
    23581 tgagaaacgt cgtattccgg ttacgttgga tcaaatccca ccggagatgg tgaaagcctt
    23641 tatcgcgacg gaagacagcc gcttctacga gcatcacggc gttgacccgg tggggatctt
    23701 ccgtgcggca agtgtggcgc tgttctccgg ccacgcgtca caaggggcga gtaccattac
    23761 ccagcagctg gcgagaaatt tcttcctcag tccagaacgc acgctgatgc gtaagattaa
    23821 ggaagtcttc ctcgcgattc gcattgaaca gttgctgacg aaagacgaga tcctcgagct
    23881 ttatctgaac aagatttacc tcggttaccg cgcctatggt gtcggtgctg cggcacaagt
    23941 ctatttcggt aaaacggtcg accaactgac gctgaacgaa atggctgtga tcgccgggct
    24001 gccgaaagcg ccttccacct ttaatccgct ctactcaatg gatcgtgccg tcgcgcggcg
    24061 taacgtcgta ctgtcgcgga tgctggatga agggtatatc acccaacaac agttcgatca
    24121 gacacgcact gaggcgatta acgctaacta tcacgcgccg gagattgctt tctctgcgcc
    24181 gtacctgagc gaaatggtgc gccaggagat gtataaccgt tatggcgaaa gtgcctatga
    24241 agacggttat cgcatttaca ccaccatcac ccgcaaagtg cagcaggccg cacagcaggc
    24301 ggtacgtaat aacgtgctgg actacgacat gcgccacggc tatcgcggcc cggcaaatgt
    24361 gctgtggaaa gtgggcgagt cggcgtggga taacaacaag attaccgata cgctgaaggc
    24421 gctgccaacc tatggtccgc tgctgcctgc cgcagtcacc agcgccaatc ctcagcaagc
    24481 gacggcgatg ctggcggacg ggtcgaccgt cgcattgagt atggaaggcg ttcgctgggc
    24541 gcgtccttac cgttcggata ctcaacaagg accgacgccg cgtaaagtga ccgatgttct
    24601 gcaaacgggt cagcaaatct gggttcgtca ggttggcgat gcatggtggc tggcacaagt
    24661 gccggaagtg aactcggcgc tggtgtcgat caatccgcaa aacggtgccg ttatggcgct
    24721 ggtcggtggc tttgatttca atcagagcaa gtttaaccgc gccacccagg cactgcgtca
    24781 ggtgggttcc aacatcaaac cgttcctcta caccgcggcg atggataaag gtctgacgct
    24841 ggcaagtatg ttgaacgatg tgccaatttc tcgctgggat gcaggtgccg gttctgactg
    24901 gcagccgaag aactcaccac cgcagtatgc tggtccaatt cgcttacgtc aggggctggg
    24961 tcagtcgaaa aacgtggtga tggtacgcgc aatgcgggcg atgggcgtcg actacgctgc
    25021 agaatatctg caacgcttcg gcttcccggc gcaaaacatt gtccacaccg aatcgctggc
    25081 gctgggttca gcgtccttca ccccaatgca ggtggcgcgc ggctacgcgg tcatggcgaa
    25141 cggtggcttc ctggtggacc cgtggtttat cagcaaaatt gaaaacgatc agggcggcgt
    25201 gattttcgaa gcgaaaccga aagtagcctg cccggaatgc gatattccgg tgatttacgg
    25261 tgatacgcag aaatcgaacg tgctggaaaa taacgatgtt gaagatgtcg ctatctcccg
    25321 cgagcagcag aatgtttctg taccaatgcc gcagctggag caggcaaatc aggcgttagt
    25381 ggcgaagact ggcgcgcagg agtacgcacc gcacgtcatc aacactccgc tggcattcct
    25441 gattaagagt gctttgaaca ccaatatctt tggtgaacca ggctggcagg gtaccggctg
    25501 gcgtgcaggt cgtgatttgc agcgtcgcga tatcggcggg aaaaccggta ccactaacag
    25561 ttcgaaagat gcgtggttct cgggttacgg tccgggcgtt gtgacctcgg tctggattgg
    25621 ctttgatgat caccgtcgta atctcggtca tacaacggct tccggagcga ttaaagatca
    25681 gatctcaggt tacgaaggcg gtgccaagag tgcccagcct gcatgggacg cttatatgaa
    25741 agccgttctt gaaggtgtgc cggagcagcc gctgacgccg cctccgggta ttgtgacggt
    25801 gaatatcgat cgcagcaccg ggcagttagc taatggtggc aacagccgcg aagagtattt
    25861 catcgaaggt acgcagccga cacaacaggc ggtgcacgag gtgggaacga ccattatcga
    25921 taatggtgag gcacaggaat tgttctgatt aaaaaggcgc ttcggcgcct tttcagttgg
    25981 ctgacaaagt gcatttgttt atgccggatg cgggtgaacg cgttatccgg ctaacaaaat
    26041 cgtgaaaaaa tcaatatatt gcaggaaacc cataagcctg ataagcgttg tgcatcaggc
    26101 aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg
    26161 cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc atatgcgcca
    26221 gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat tccgggtaga
    26281 gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc atgctgagct
    26341 tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc tcgcggttag
    26401 cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct aattcgtagg
    26461 attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcgtccaca atcggcacaa
    26521 tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc acgccattgc
    26581 tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca gtttcaacat
    26641 tcagaatggt gggtttttgt aatgatttgc tcatcgtggg atctatgctg tgaaatcagc
    26701 ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta tttacattta
    26761 tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt gaaataactc
    26821 caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt tactgtgatg
    26881 tgcttaatga tgctcaagtt aaactccacg cttgccgata gccaaccgca gaatcatgta
    26941 ttgtgtctgg tgcgactgac cacgcctgac agactaagta agatggggaa agcatgagca
    27001 ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga tggctgataa
    27061 aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat gcgcaaacgc
    27121 gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg
    27181 tattacaggt gcctggccca acgggagcca gcgcggcacc gatctctctg gcgctgaatg
    27241 ccgaaagcaa caacgtcatg atgctgacac acgctatcac gcgttacggc atctctaccg
    27301 acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg ccccctttct
    27361 gggaacaata catcaacgat gagaataccg ttgaactgat ttataccgat tcgctgccgc
    27421 tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg cgcggctatg
    27481 ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt gagcaaatcg
    27541 agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt ccgcgcgggc
    27601 tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc ctgattaccc
    27661 cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc gcaggtctgt
    27721 gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt ctgcgcggta
    27781 caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac aatatttcac
    27841 tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct caggatctcg
    27901 gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag gggcgatatc
    27961 tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc agtacgatta
    28021 tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctgggtcccg ctggatatgc
    28081 cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc accagcgtaa
    28141 aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc ggtacgggaa
    28201 tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct tttttaccgt
    28261 ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca gaatctgaac
    28321 tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac cctaaaccgg
    28381 aagatgaatc tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc ggcatggtat
    28441 tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct gccaaagatg
    28501 actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac tgggacgcgc
    28561 tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta cgcccggtga
    28621 gtgcagaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc acgcatgaaa
    28681 cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg attgtcagcg
    28741 acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac gactacccgc
    28801 cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat acgccgttta
    28861 acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag catattggcc
    28921 ttcatccgat cccggatcgt tccggactgt ggcgctatct cagcaccaca ttgctgctac
    28981 tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt taccagcgtc
    29041 atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac ccgcaactga
    29101 tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat gctaccctgt
    29161 cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc ctggcaggac
    29221 gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt cgataactat
    29281 ttctggcaaa agctggtgcc tgaaacatgg ggcgccaaaa acggggttac gccacaggaa
    29341 gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa ctggtactgt
    29401 cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac cgagatggga
    29461 ccgcgtgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa agccagcggt
    29521 aagcagcgaa ttttgctcac caatgcgcat ccgcacaacc tggcggtaaa acttgagcat
    29581 accggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg ttatccgaaa
    29641 gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc tgaaagaacg
    29701 ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg tattcgttac
    29761 tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca acgccatccg
    29821 tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg ccatgaaaga
    29881 gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt tttataaaac
    29941 ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg ggcagcgcag
    30001 caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg gaaatgacga
    30061 acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg aggcagcctt
    30121 gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga tggcacgtaa
    30181 acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag agcgccgcga
    30241 cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag agatgattat
    30301 gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc gcggcgaact
    30361 ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc cgcagcccgt
    30421 taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta cgctgaagtt
    30481 tgatggtgat atcaccgtac agctgcaagg cgacggtccg atgaatctgg cggttattaa
    30541 cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa ttccagaaaa
    30601 tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta ccccgagcga
    30661 aggcgaacgc tatcagggcg tggttggtct ggaaggtgat accctggcgg cctgcctgga
    30721 agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca ccggcgacgt
    30781 agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc aaaatgccca
    30841 gcaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg aagaactgct
    30901 gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg tgacggttta
    30961 cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg ccgatgcgct
    31021 gaaaacgctg cctgatgaag aagttgatag catcctgacg gaagatggcg aaattgacat
    31081 gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg ctgaaatccg
    31141 caacaacgcg tctccggcag atccgcaagt tcattaatga gtatgtccgg cagagagggc
    31201 tctgccggat tttacgacgt aatggcctga tgtgctgcgc ttaccaggcc tgtgaaataa
    31261 atcgcaaatt attgttggtc agatacagcg tcgtatccgt taaaagagtt gtcacttact
    31321 taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt tcggcctcgt
    31381 ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt cgttcggaag
    31441 taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat agacaatgac
    31501 tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat tcagatcttg
    31561 ctcgaccagg acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat
    31621 taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta acacattttc
    31681 ggctaatact ttttccgata cctgttcctg aagatgttgc tctccatcta acgccatcag
    31741 cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca gcgactcaag
    31801 cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct ggtctggcgt
    31861 gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca tactatccag
    31921 caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg caaggttcac
    31981 tttgccctga agaacaaggg gattttgccc attacgccag acgatactga caaaataccc
    32041 cagcgaccac accagtaaca ccagcaccgc cagcgcgccc tgcaagcggt cgacagtcca
    32101 gccgtactga gcgacccgca gccataacgc ccaggcggcg acgaacacat acagcggagc
    32161 aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa gtgacgcttt
    32221 ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg ttaacagcaa
    32281 cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca gagtgatgat
    32341 aaacatcagg gttagcaatg atactaacgg cagcaacccc gtggcgatta acgtgaacaa
    32401 cttttgaata gagtcgatta aacgtgactg tgtgcgcgcg agaatcaccg ccagtgcggt
    32461 aaccagacct aatgtaagat acataaacca gtcggttgca aaaaagagcg tattgaaaaa
    32521 cttgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccaccc acgtcaggcc
    32581 attggcgaga aaaatcacca gtaatataag tacgttatgc catactgact tgtaaaagta
    32641 gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg gtagcaacaa
    32701 cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag ctctccaggg
    32761 gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg caataaacac
    32821 cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac
    32881 ggatgaaaac gcaacggtcg caggcacgcc ataaacaatc caactgtgat tttttccggc
    32941 aagccaggcg atcaacaggt agcaaaccag cccctgaagt aatccggtag caatcatccc
    33001 ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt
    33061 gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa
    33121 gttagcgtgg tgaatcgata ctttaccgat tgaatttgca tcaatttcat taaggaatgc
    33181 gattccactc acaatattcc cgccatataa accaagattt aaccttttga gaacattttc
    33241 cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt
    33301 tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca
    33361 cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc
    33421 gttaacaatg gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat
    33481 atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca
    33541 ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc
    33601 accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc
    33661 tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg
    33721 cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac
    33781 gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc
    33841 tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga gctggcaggt
    33901 ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa
    33961 cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt
    34021 ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg
    34081 ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggtga gaaaggcgat
    34141 gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa
    34201 cgtcgcctga ttggtgatga cgagcacggc tgggacgatg acggcgtgtt taacttcgaa
    34261 ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac
    34321 gctatccgtc gcgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac
    34381 tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccgatcta tcacatcgat
    34441 aacattgtta agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct
    34501 gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac
    34561 ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg
    34621 ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac tcagtacgca
    34681 gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc
    34741 tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc
    34801 ctcaacggtt cgctggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc
    34861 ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct
    34921 ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat
    34981 aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga
    35041 tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg
    35101 cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag
    35161 ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg
    35221 gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc
    35281 actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg
    35341 accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat
    35401 ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga
    35461 cagcgggtgc attttcacgt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc
    35521 atagccactt tcggcagcaa tcacctcacc gagtactgca ttaagatccg ccatttccat
    35581 cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc
    35641 ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt
    35701 cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag
    35761 cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac
    35821 ggaacgcacc tcggacgcgc catactcacg cagcggcggc ggaataatcc ctttaccaac
    35881 ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca
    35941 cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc
    36001 gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt
    36061 cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag
    36121 ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc
    36181 ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa
    36241 agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat
    36301 acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt
    36361 cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa
    36421 cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc
    36481 ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct
    36541 tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa
    36601 tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg
    36661 ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg
    36721 cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct
    36781 tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acgaatacgg
    36841 gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg
    36901 cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg
    36961 ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc
    37021 agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg
    37081 acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc
    37141 atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt
    37201 tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct
    37261 ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat
    37321 ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat
    37381 tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta
    37441 tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa
    37501 aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa
    37561 taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcatggaaaa
    37621 tctcaaaatc gtcgattact cccctcagca ggaaggcaag gtcttttttg gcgcatgggt
    37681 ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat
    37741 ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa
    37801 agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgagct ggtatgtcaa
    37861 tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt
    37921 tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac
    37981 catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca
    38041 ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta tcgcacgtta
    38101 tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct
    38161 gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca tttccgagca
    38221 aggcaaactc accgatgatc tggcgaaggc catcaacgcc acactaagca aaaccgaact
    38281 cgaagatctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga
    38341 agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt
    38401 cgccgctgca caatatgttg atgcagataa aggcgtggca gataccaaag ccgcgctgga
    38461 cggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt
    38521 gcgtgattat ctgtggaaga acgcgcatct ggtctcaacc gtgatgagcg gtaaagaaga
    38581 ggaaggggca aaattccgcg actatttcga tcatcacgaa ccgctgtcca cggtgccttc
    38641 tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc tttcgctgaa
    38701 tgccgatccg cagttcgaag agccgccaaa agagagctat tgcgagcaaa tcatcatgga
    38761 tcatctcggc ctgcgcctga acaatgctcc ggcggatagc tggcgcaaag gcgtggtgag
    38821 ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gcaccgtgcg
    38881 cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat
    38941 ggcggcccct gccggactgc gtgccacgat gggcctcgat ccgggtctgc gtaccggggt
    39001 aaaagtagcg gtggtcgatg ccactggcaa actggtggcg accgatacca tttacccgca
    39061 caccggacaa acagcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa agcataacgt
    39121 tgaactggtg gcgatcggca acggtacagc ttcccgcgaa accgaacgtt tctatctcga
    39181 cgtgcagaag cagttcccga aagtgaccgc gcagaaagtg atcgtcagcg aagctggcgc
    39241 gtcggtttac tcggcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct
    39301 gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat
    39361 cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc
    39421 ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac
    39481 cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat
    39541 cgtggcctgg cgcgatgaga acggccagtt caagaaccgt cagcaactgc tgaaagtcag
    39601 ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta accacggtga
    39661 taacccgctg gacgcctcta ccgttcaccc ggaagcctat ccggtggtgg aacgcattct
    39721 ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct
    39781 gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa
    39841 agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga
    39901 tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg ctgtcaccaa
    39961 cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat
    40021 ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat
    40081 tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgaccatgcg
    40141 tctggacgag cagcctggcg aaaccaacgc ccgtcgcggc ggcggtaatg aacgcccgca
    40201 aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc
    40261 gatgatggat gcgctggcgg cggcaatggg caaaaaacgt taaacgccct gattgacccc
    40321 taaacctgcg cgataaggtg tccacgccgc atctggcaac gaacgccttg tctgacataa
    40381 aagtgccgga gaatatctcc ggcattttta ttccacagcc aaactcacaa tatattccgg
    40441 caacatttat cacttcatta acagctgaaa ccttaattaa acattagcca gtcctggtaa
    40501 ttcgctattc gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc
    40561 agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag aaaccattct
    40621 cattatcatt gtgctgttga ttatttaatc tctccttcgt tggcaaatca tctggtctca
    40681 tgtcgctgtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct
    40741 atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg
    40801 gcatatcgcc aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg
    40861 cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta
    40921 cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac
    40981 aacaatgaaa aaattaacca taggcttaat tggtaatcca aattctggca agacaacctt
    41041 atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga
    41101 acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg
    41161 cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca
    41221 ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga
    41281 gcgtaacctg tacctgacgc tacaactgct ggaactcggc attccctgca tcgtggcact
    41341 gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc
    41401 gcgtcttggc tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct
    41461 caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca
    41521 gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa
    41581 acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc
    41641 cggtgaagcg tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg agatggacga
    41701 tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt
    41761 aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct
    41821 caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc
    41881 tatcaacatc ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt
    41941 gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct
    42001 cgcccagggc ctgggtggcg gcattaacac cgtgctgcca ctggtgccgc agattggcat
    42061 gatgtacctg ttcctctcct tccttgagga ctccgggtat atggcgcgtg cggcgtttgt
    42121 gatggaccgt ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt
    42181 cggtttcggt tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg caccgcgtga
    42241 acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt
    42301 cgcagtattc gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta
    42361 tatgctgggt attgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg
    42421 cggtgaagcg acgccgtttg tcatggagtt gccggtctat catgtaccac acgttaaaag
    42481 cctgattatc cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat
    42541 catcatcgtc agcattttcc tgagcgcttt caacagcttc tcgctgagcg ggaaaatcgt
    42601 cgataacatc aacgactcgg cgctggcgtc cgtcagccgg gtgatcaccc cggtcttcaa
    42661 gccaattggc gtgcatgaag ataactggca ggcaacggtt ggcctgttta caggtgccat
    42721 ggcgaaagaa gtggtagtgg gtacgctcaa caccctctac accgcagaaa atattcagga
    42781 cgaagagttc aatccggcag agtttaacct cggtgaagag ctgttcagtg cgatagatga
    42841 aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc
    42901 cagcaaaggc gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg
    42961 tagcgcagca gcagcttaca gctacctgat tttcgtcctg ctgtatgtac catgtatctc
    43021 ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg
    43081 ggggctgaat atcgcttact cgctggcaac attgttctat caggtcgcca gctacagtca
    43141 gcacccaact tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat
    43201 cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc
    43261 ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt
    43321 caggtgcgcg atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca
    43381 ttgaacactc cacagccaat gattaacgcc atgctgcaac aactggaaag tatgggcaaa
    43441 gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa aagctgcccg
    43501 gaaggaaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccttactc catcgcctgt
    43561 tttggaaagc ccggtatgcg tctgcatccg ggcttttttg cgtgcggctt tccataaaaa
    43621 tgcaactctt gcagcacggc gtaagttcct ttgaaagcat ctcgcaggga tgaaaactcg
    43681 ctaatacaca ggtgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa
    43741 cgccatgagc aaaaagcaga gttccacccc acacgatgcg ctgttcaaac tctttttacg
    43801 ccaaccggac acggctcgtg attttcttgc gtttcattta ccggcaccca ttcacgcgct
    43861 ttgtgatatg aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga
    43921 aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat acatctattg
    43981 tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc
    44041 tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga tggtggtgcc
    44101 attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg
    44161 tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt
    44221 caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat
    44281 tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg cctgcttatt
    44341 aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac
    44401 cggcgacgcg gtacgtttta acgattttat cgacggcgtt gccgaacgtt caccgaaaca
    44461 caaggagagt ttaatgacaa ttgcggaaag attgcggcag gagggggaac aatccaaagc
    44521 cctgcatata gccaaaataa tgcttgaatc cggagtccct cttacagaca tcatgcgctt
    44581 taccggggtg tcagaagaag agttggctgc ggcgagccag taaatttctg tctcgccatt
    44641 tcaaaagttg cctacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga
    44701 tgcgaaataa acggcgcatg ggccgctttg gcgaagatat atgattcgct gtgaggccaa
    44761 agtttatcca gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat
    44821 cgcaaaaacg gcatggacac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt
    44881 tccagcccgc cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc
    44941 aacgcccgcg catcctggcg cgccgtttca gtccccatgg tttgtaacgc caggaaccgc
    45001 tccactgtac gctgaaaatc atcactgagt tgctgctgaa atcccgccag tacgtccggt
    45061 tttatccccg gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc
    45121 gcctgaacac gctcgggatg ggttaaaaca atttggcttg ccaccagccc gcccagactc
    45181 cagcctaacc aaatggcttt atcaggtgcc tgttgcagca cggcttcggc catatcagca
    45241 agtgacagcg caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc
    45301 gtaaaatgcg agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat
    45361 ccgtgcagca gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc
    45421 atccgctatt gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta
    45481 tgctggctat gccgaatgcc actggcgtta ggtcattggg ggatttgttc ggtctgttca
    45541 cgcgccaccc gcacagataa aacgttatgc ccacaatgtg gattacccgc cacacactcc
    45601 catcttccct gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt
    45661 gccgactatg cgccgccgtt aagtccgctt atccaccagc ttaaattttc ccggcgcagt
    45721 gaaatcgcca gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc
    45781 accgggttgc aattgccgga tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg
    45841 cgtcggggat ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcattgc
    45901 caatgggata gcgaagccgt cacacgtaca cgggccactg cgacccagca ttttctcagt
    45961 gcccggctgc gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt
    46021 cgccatatgg tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg
    46081 cagttgcttt tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg
    46141 tagagcctcg atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac
    46201 tatgatccgt atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga
    46261 agaagggaca caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg
    46321 tgtttcttat tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct
    46381 gctgaccgcg tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgactt
    46441 tgttaccgac cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca aaatgcgtaa
    46501 agtggcagac gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa
    46561 cccacagctt gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta
    46621 cgccattctg caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa
    46681 agaagggatc gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct
    46741 caccgaacac cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg
    46801 cccgatgcga cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggca
    46861 cagcattgct ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca
    46921 taatttaaat ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca
    46981 catgggtacc aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag
    47041 tcgtggtgcc gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat
    47101 tgacgttacc cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat
    47161 cggtgtaatc ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct
    47221 cgtcctcgtg gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg
    47281 ctccatcaaa gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg
    47341 cgcaatgctg ggcaaaattc tggcagactg cggtggcgca caacgtatcg ccaccacgct
    47401 gattgccaaa tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt
    47461 tggttttgcc ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat
    47521 cgcggcttct gcgaatattc cactgttgta tgttggtgta ccaatggcag ctgcactgtc
    47581 tgtgacccac ggcttcctgc caccgcatcc gggtccgact gcgattgcca ccattttcaa
    47641 tgccgatatg ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct
    47701 cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat aagccaattc cggaaggtct
    47761 ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac
    47821 ctctctggtg ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa
    47881 aggtcacgct ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg ctacgctgat
    47941 tgccgtgctg attgccatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat
    48001 taacgacacg ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg
    48061 tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat
    48121 gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg
    48181 tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct
    48241 gattgcaacg acgggtgtta gcccggagct gatggttatt gcggtgggtt ccggtagtgt
    48301 gattttctct catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac
    48361 tatcggcgag accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct
    48421 ggtaggctgt ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca
    48481 tccggcatca accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc
    48541 cttcagcaac ttgttcacgc catcatcggc aaacatcgac tcaagcgttg cggaaagctt
    48601 gcgtcgccag tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc
    48661 cagccagtct tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg
    48721 caaaccacgg ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt
    48781 tggcagacaa ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc
    48841 ctgatacaga ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt
    48901 tagatccccg ctctcccagt aaccgcgtag cgtaggcagg tcatgtgtcg ccgcaaccgc
    48961 catcgactgc tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa
    49021 atagagcact ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg
    49081 tacggtaccg agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag
    49141 aatcgagagc agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc
    49201 atacggtatc caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca
    49261 gttttgcata ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat
    49321 atgcgggtcc attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg
    49381 cgcgccaacc gacgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc
    49441 ttccgctacg ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct
    49501 tatctcccag caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc
    49561 gacgtcatca cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttctgata
    49621 catctctggc catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg
    49681 tagcgcatca aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg
    49741 aaacgcggtc atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc
    49801 tgtcattttt agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg
    49861 cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa
    49921 atcttcaacg gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct
    49981 gtatgggctg gcactctccg gatttgccgg atagagcgca tgaatcgggt tcaggccgat
    50041 gaacgacccg ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc
    50101 aataccccag tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt
    50161 ttgtttattc agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcacccggca
    50221 atgcgcgcgc tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg gcagcttcgt
    50281 cggtagattg aacgctttgc cccccgttac atggcctttg tactgtgttc cttcttcggt
    50341 ggtcagcagc cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct
    50401 ggtataaacc atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg
    50461 catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc
    50521 attgatgtaa ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc
    50581 catcgagctt ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg
    50641 atccgagcta aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc
    50701 ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc
    50761 catcaccaga tacggatcgc cgccttgttt gccgatactg tgcagcatct ggtcgaaggc
    50821 atgcttatcg ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac
    50881 cttatctttc ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg
    50941 ttccacggta tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac
    51001 gttcgcacca tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc
    51061 ggtaccggaa gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat
    51121 cagtttttcc gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc
    51181 aaccagtgga tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt
    51241 cgccaggtag tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc
    51301 agcctgcggg ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg
    51361 ctggcgtttg tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat
    51421 ctcaataccg gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat
    51481 ctcgcgatat tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg
    51541 atcgagatcg ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg
    51601 gttgcactgt ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt
    51661 cggccatagc tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc
    51721 cgcaacaccg ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt
    51781 atcgtgcacc accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac
    51841 cagcgtttta aagcgagtat taatttcgtt aataatctgc atatggcgcg gcagtaagcc
    51901 tttcaccagt ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta
    51961 agcgaaagtt ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat
    52021 cagcacgcgc agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac
    52081 ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc
    52141 cgctaccgaa caggcacact ggaagtactg ctgcatcaag cgcagctttt taccggcagt
    52201 atggttgtcg tttggataga gaactttggt cagtttttcc gcattgatgc cctgctgttc
    52261 ggcacgcagg aaatcaccgt cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc
    52321 ctgccacaga cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca
    52381 cgcttgaccg gtaatggtaa actccggctc ccagcgtccg tctttcgtca ctttaccgcc
    52441 aatccctacc tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg
    52501 ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata
    52561 ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa
    52621 gcacgccgcc agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag
    52681 caggtccgtc agattgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc
    52741 gagattcaac aggttgttgc ccgtcaggcg accaatcaaa aactccattg agatgtagtt
    52801 aacatgtcgc tgattcgcca ccggcttggc gaatggctga gcacgcagca tttcggccag
    52861 tgcttcactc actgctagcc accactggcg aggagtcatt tcagccgcag aatttaagcc
    52921 ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg
    52981 tgacatagga gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg
    53041 ctcgatgttg accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag
    53101 caaggaaatg tgatctcaac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc
    53161 aatgcaatac agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc
    53221 aaaaagcgga aggtaactct ataaattaag taaaggagtg aaacagtttc ataagtaaaa
    53281 tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca
    53341 ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac ataagggaaa
    53401 ccagtattca cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg
    53461 tgacacagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct
    53521 ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca
    53581 cagtgaagtg attaactatg ctgattccgt caaaactaag tcgtccggtt cgactcgacc
    53641 ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg
    53701 cgctgatcac gagtcctgcg ggctacggaa agaccaccct catttcccag tgggcggcag
    53761 gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa gagcgtttcg
    53821 ccagctatct cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga
    53881 cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg
    53941 agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta
    54001 atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc
    54061 tggtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt gttcgtgatc
    54121 aactgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt
    54181 ttgattgccg tctgtcatcg ccgattgaag ctgcagaaag cagtcggatt tgcgatgacg
    54241 tttccggttg ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact
    54301 cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc
    54361 tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg
    54421 ccattttgcg ctcaatgaac gatgccctca tcacccgtgt gaccggcgaa gaaaacgggc
    54481 aaatgcgtct cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg
    54541 gcgagtggtt ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg
    54601 aactggcggc ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg
    54661 gatttcccag cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg
    54721 atattctgct taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag
    54781 agtcgcttaa ggccctgccg tgggacagtt tgctggaaaa tccgcagtta gtgttattgc
    54841 aggcgtggct aatgcaaagc caacatcgct atggcgaagt taacaccctg ctagcccgtg
    54901 ctgaacatga aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc
    54961 gcgcccaggt ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg
    55021 cactggaaga gctgccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg
    55081 gtgaagtgct gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg
    55141 aacagatggc acgccagcac gacgtctggc actacgcctt atggagttta atccagcaaa
    55201 gtgaaattct gtttgcccaa gggttcctgc aaaccgcgtg ggaaacacag gaaaaagcgt
    55261 tccagctgat caacgagcag catctggaac agctgccgat gcatgagttt ctggtgcgca
    55321 tccgagcaca actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta
    55381 gcgggattga agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt
    55441 tgattcaatg ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ctgaaccgtc
    55501 tggaaaacct gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag
    55561 tccgggtgat ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc
    55621 atacggctaa accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg
    55681 cccgcgcaca aatcttgctg ggcgagtttg aacctgcgga aattgttctc gaagaactca
    55741 atgaaaatgc ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctta
    55801 atcaactgta ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat
    55861 taaaactggc gaatcgcacc ggatttatca gccattttgt catcgaaggc gaagcgatgg
    55921 cgcaacaact gcgccagctg attcaactca atacgctgcc ggaactggaa cagcatcgcg
    55981 cacagcgtat tctgcgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg
    56041 agaatttcgt tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc cgcaccagcc
    56101 cgttgaccca acgtgaatgg caggtcctgg ggctgattta ctccggttac agcaatgaac
    56161 aaattgcggg cgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt
    56221 accaaaaact cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga
    56281 agatgatggg ttatggggtg tgagtttagc cggataatgc attggatccg gtttacatct
    56341 ttacatcatt caatactcac ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc
    56401 ggcaagaaac gctccaccac cgcgatattg gtcagcagat ggcatgaagg atgggcgacc
    56461 ttaaattctc ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc gagatattcc
    56521 cccaccgccg ctgggcttgc caggtagcgt ttcacctctt tcactaactg cgcggctacc
    56581 acctcggcac tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat attttcactt
    56641 tcgacttcca gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc
    56701 tgttcatgca gggaaaaact ccccgccagt gtagcgattt cacgctcagc aacatggcgc
    56761 ggcacaccag ctaatagaac ttctccacgc atctgcacaa tgttcccgcg ctcgccaagt
    56821 tgcaaggtgt taaacgatgc cacgggcgag acttccgttg ccaccacgcc gcctccggca
    56881 ggataaaaac cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc
    56941 ggctccagca cccggcggat aaaatccgca ggcggggccg acgggttatc ggtgcctccg
    57001 ctcacttcaa cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc
    57061 accagcgtac agcttccggc actaccgata gcaaagcggt actcgccgcc gcgcacggtg
    57121 ccaggccgga agaccagacg ctgcgacccc agctccgcgc cttccaccgt tgccctacaa
    57181 atttccgcag ccgcttttac cgcggtcaga tgctggcgca acagccccgg tttcgcccgc
    57241 ccggcacgaa tgctggtgat ggtaaatggc tggccggtta tcatcgacag gctcagcgcc
    57301 gagcgcagga tctgcccgcc accttcgccc tgtgcgccat ccagcgcaat catccttttc
    57361 atcatttatc cttttacgca caccacctga cgcagggtat agataacttc caccagatcg
    57421 ctttgtgccg ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact
    57481 tcggcatctt tacggcattc cacatgcgcg gtggcacgaa tttgatcttc cacgctgaac
    57541 agttttttcg ctttagtacg gctcattacc cgcccggcac cgtggctgca cgaacagaac
    57601 gactcttcat ttcccagccc acggacgata aagctttttg ctcccatcga accgggaata
    57661 attccatatt gaccagcacg cgcagacacc gcgcctttac gcgtcacgta gatctcttca
    57721 ccaaagtgct gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc
    57781 gtttgtggct gtctgaccgt tttctgcgta atgctctgca acgccgttac cacgttttcc
    57841 atcatcgcat cgcggttaag gctggcgaaa agctgcgccc aggccacggc tcttaggtaa
    57901 tcatcaaagt attccgtacc ttccataaag tacgccaggt cgcgcgatgg caacgtttca
    57961 agctggtcct gcatctcttt ttgtgccaaa tcgataaagt aagtcccgat ggcgttacca
    58021 attccgcgtg aaccggagtg cagcataatc cacacctggt ccgactcatc aaggcagatt
    58081 tcaataaagt ggttacccgt tcccagcgtt cccaggtgtt tatagttgtt agtgttaagg
    58141 aaacgcggat atttttgcgt taaccactga taaccggctt caagctcagc ccatttcgca
    58201 tcgacgttaa caggcgggtt ttcccatgcg cctttatcac gtttacaacg gccagtggta
    58261 cgtccgtgcg gcaccgcagt ttcaatcgcc tgacgcagct ctgccaggtt ttcaggcagg
    58321 tctgccgccg ttaacgcggt acgcagcgcg ttcattccac agccaatatc cacgcccacc
    58381 gccgccggaa taatcgcccc tttggtcggg atcacgctac caatggtgga acctttaccc
    58441 aggtgtacat caggcattac cgcaatatgt ttgaaaataa acggcatctt cgccgtatta
    58501 ataagttgct gacgcgcatc ggcttctacc ggcacgcctt tggtccacat ttttaccggg
    58561 gcattttcag tggtcagtaa ttcgtaattc attttgtttt tctcttttcg ttgtttgctg
    58621 tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt ttttaattca
    58681 ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac aaaaccaatt
    58741 caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa cagtggcttt
    58801 tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcgaaatg
    58861 gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc tggaattgtt
    58921 gcacgacacc cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg ccagcgtttc
    58981 gccagaaaca caagtcgtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga
    59041 ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga
    59101 ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc
    59161 ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca aaaaagaaca
    59221 gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat
    59281 cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt ccggcattgc
    59341 cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgatcaaatc
    59401 ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc tggcgcgacg
    59461 catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg aagtgaactg
    59521 cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa aaggcgcgtt
    59581 taccggggcg cgggaatctc gtgaaggttt attacgcagc gccaacggcg gaatgttgtt
    59641 tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa aagccattga
    59701 agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat
    59761 cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc gcgaagatct
    59821 gtacacgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga
    59881 tattgaaccg aacctggatt atgaagtgga gcgccacgcc tcactcactg gcgacagcgt
    59941 gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc cccaggcaac
    60001 atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac
    60061 tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc gctataactg
    60121 gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga
    60181 tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct
    60241 ttccgcagcc ggacgccagc tttttgacgt ttcgcgccag ggcaaagcca gcgtcaatga
    60301 cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga
    60361 tcagcacagc tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg
    60421 cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga ccatcgcgtt
    60481 acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct caataatggc
    60541 gcgtttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tatcgatgcc
    60601 gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc
    60661 gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa tgcggaaatc
    60721 ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac gcaaattgct
    60781 gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagtgt
    60841 cgagccattg gggatttgct ccgccacttt gcgggcaatc ctctcttttt cttcggtctg
    60901 ggtggcttta cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcaccgcc
    60961 atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc gaatagtctg
    61021 cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct gctgtttaac
    61081 cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt
    61141 ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaacccgg
    61201 cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc
    61261 agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt
    61321 cgccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc
    61381 acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg agagtaataa
    61441 caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac caccaccaga
    61501 gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc
    61561 agaactcaaa tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat
    61621 cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcacc acccaggtta
    61681 ccggacctgc gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcagaccac
    61741 tgccggtatg gcccgcctgc cagctggccg ccagataacg cggatctgcc gggttttcga
    61801 gaaaacgcgc cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat
    61861 cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccata taatcaacaa
    61921 acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt
    61981 attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa
    62041 ctgacgctgc caggcttcaa aaccgccgtc aatgctatag accacatcgt agccctgttg
    62101 cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca ccatcaccgg
    62161 agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc
    62221 ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc
    62281 tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaaca gatccatgcg
    62341 tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag
    62401 ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt acctaaagcg
    62461 cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag
    62521 ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc
    62581 gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct
    62641 tgtgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac
    62701 tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct
    62761 ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg
    62821 tggatgattc ggattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg
    62881 ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat taagcgcgga
    62941 ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg
    63001 gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa
    63061 aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa
    63121 gcgcgcggcc tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg
    63181 catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgt
    63241 gtgcataccc agaagcaggc ctacattctg caaaacgaag ataaacgtat tgtgttcgtg
    63301 atcccgtgga tggacgaatt ttccatcatc ggcaccaccg acgtagagta taaaggcgat
    63361 ccgaaagcgg tgaagattga agagagtgaa atcaactatc tgctgaaagt gtataacgcg
    63421 cactttaaaa aacagcttag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg
    63481 ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac ccttgatatc
    63541 catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac
    63601 cgaaaactgg cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg
    63661 gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac
    63721 gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg tcattacgca
    63781 cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat aagcgacctc
    63841 ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa
    63901 tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat gtggctgaat
    63961 gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg
    64021 ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga ttccttagac
    64081 tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac ttaagcacgt
    64141 tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat ggcaataata
    64201 tccccatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc aaggctgcgg
    64261 ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat ttcggatatt
    64321 ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga gaattaacca
    64381 gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt caggaggcgt
    64441 tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg catattcaac
    64501 cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg gaaaggagaa
    64561 tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa taaccgtcgg
    64621 ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat aaagccggaa
    64681 tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc gcataaacac
    64741 tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc gggctgtttt
    64801 agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt atttctagcc
    64861 caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc tctgatataa
    64921 tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa gccgggaact
    64981 gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa catttccagc
    65041 atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata ctggcaggca
    65101 aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc aaggttgata
    65161 cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac aaattccagt
    65221 aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc tgggctacta
    65281 ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg taatgccttt
    65341 tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg cacccgataa
    65401 cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggca gggttgcata
    65461 gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga gcattcgccg
    65521 cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg caactattgc
    65581 agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg tagagtttca
    65641 gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat ttccaggcat
    65701 ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt ttaagcgggc
    65761 gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt acggtgacgt
    65821 catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag gggtcaaatc
    65881 caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg gaaaatcccg
    65941 gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga agcaatccct
    66001 caagaccgtc agcaccgacg aacttttccc cttcatccag ggcctggtta ataagttgct
    66061 gaacaatgcc ttcatcccag tatgccgatt tgtagttggc aatcgtaata tccacgcctg
    66121 tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc atttttatta
    66181 tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc accaaactct
    66241 gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc gtattttccc
    66301 gcgttatcat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg ttttcggcag
    66361 tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc atttgtgttg
    66421 cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg gcaacgactt
    66481 taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc atgcggacat
    66541 ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg cgttgccagg
    66601 tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca ccactgaaga
    66661 tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg acgatgaaaa
    66721 caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc tcagcgcgtt
    66781 ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct ttaacaatcc
    66841 tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga aaatcttccg
    66901 gtataacgct taaagcataa aatgcagaag cataaattgg gtacgccgcc gtagcaccat
    66961 cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt ggtgtcagtt
    67021 gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc acaccgacgc
    67081 cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca agcatgagaa
    67141 ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa ggatgtctgg
    67201 ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata atgactatcc
    67261 accagtactc acctccgact actgagacta acaaaacgcc cagccagggg agcataagat
    67321 cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc agcgcgatta
    67381 tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc cagacggcta
    67441 tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa aatagcatta
    67501 atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc gtgataagga
    67561 tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca accgccgcca
    67621 aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac aaaatccatt
    67681 ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact ccccgagtcc
    67741 ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga gcctttggcc
    67801 ccgttctatt tattggtgaa cttacaatct caccggatcg atatgccaga tatgatcggc
    67861 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
    67921 ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct gacaatcgac
    67981 atagctgcga taatccgcca gaacctgata gtgatcgccg aagttgatca gcgaatcaac
    68041 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
    68101 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
    68161 cagttcttcc acttcttccg cagtgttacc aaagataaag atattgtcag caccgacatg
    68221 atccagcatc tcgacgttcg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc
    68281 aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct gttcagacag
    68341 atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
    68401 tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
    68461 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
    68521 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
    68581 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
    68641 cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca gacgcttttt
    68701 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
    68761 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
    68821 ggctgaaagc gatgggttcg ctaccgctag ccagcgacgc ggcgtcacac cgttggtgac
    68881 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
    68941 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
    69001 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
    69061 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
    69121 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
    69181 gctcatcagc gtatggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
    69241 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
    69301 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
    69361 ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg
    69421 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
    69481 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga atttaccgag
    69541 gttaatttcg ctactggctt gcgcactcca caaacgcaac gtgttggtcg cgtcggtgtc
    69601 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
    69661 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
    69721 gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact ctttctggct
    69781 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
    69841 cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc ccaggccacc
    69901 gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
    69961 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
    70021 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
    70081 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
    70141 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
    70201 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
    70261 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
    70321 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
    70381 cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc
    70441 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
    70501 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
    70561 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
    70621 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
    70681 gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
    70741 gagtagcgcc agctgcccgc cctgctccag aaggcccggt aaggcttcca gcaccaggtc
    70801 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcatcaac
    70861 cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc
    70921 gcgggtgtag cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
    70981 gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
    71041 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactaa ccgccgtaat
    71101 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
    71161 atgaatatta aagaatgacc atggcaattg gatgtcattc atatgatgtg cataaaacat
    71221 gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc
    71281 cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca
    71341 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
    71401 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
    71461 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
    71521 gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtaa cgccacggcg
    71581 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
    71641 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
    71701 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
    71761 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
    71821 ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc
    71881 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
    71941 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
    72001 acacagccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
    72061 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
    72121 atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc
    72181 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
    72241 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
    72301 tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag atagtcggcg
    72361 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
    72421 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
    72481 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
    72541 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
    72601 aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca
    72661 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
    72721 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
    72781 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta
    72841 gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
    72901 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
    72961 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
    73021 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag
    73081 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
    73141 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
    73201 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
    73261 atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccaccaaag
    73321 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
    73381 cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat
    73441 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca
    73501 acgttgttaa cagggcgtga atgctgtcgc gccgccgttc aaccaggtca agagaaccgc
    73561 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
    73621 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
    73681 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
    73741 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgtagccaga
    73801 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
    73861 ccacctgata accaccagga gcgatatccc acggttcagc aattaacttc acctgcgaga
    73921 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
    73981 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
    74041 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
    74101 cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttat
    74161 cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
    74221 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
    74281 catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
    74341 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
    74401 gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag ttgatcatca
    74461 ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaagt
    74521 acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg
    74581 gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
    74641 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact
    74701 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
    74761 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
    74821 ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct
    74881 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
    74941 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
    75001 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg tcaggcttag
    75061 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
    75121 attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt tgcccggctg
    75181 gtttatgccg aagcgataat catgacgcgg taccggcgta aagttactgg caacgatgat
    75241 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
    75301 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggcg
    75361 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
    75421 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
    75481 gttacccatg aacagtagtt tcttgcccgg gaatgcccac atccagccat agtaggcgcg
    75541 caggttcgcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
    75601 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagaat
    75661 cccgaaggtc agtttatcgt gatgatactg acgataaacc gggtcgagct tcatgtagtc
    75721 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
    75781 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
    75841 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
    75901 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat
    75961 cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
    76021 cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
    76081 ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg tgccatcaaa
    76141 ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga gaatcacgtt
    76201 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
    76261 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
    76321 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
    76381 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggaacc
    76441 caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
    76501 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
    76561 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
    76621 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
    76681 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
    76741 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
    76801 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
    76861 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
    76921 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
    76981 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
    77041 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
    77101 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
    77161 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
    77221 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
    77281 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
    77341 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
    77401 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
    77461 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
    77521 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
    77581 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
    77641 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttccacggtc
    77701 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
    77761 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    77821 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    77881 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    77941 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    78001 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    78061 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    78121 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    78181 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    78241 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    78301 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    78361 atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt
    78421 ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac agggcgaatg
    78481 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    78541 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    78601 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
    78661 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    78721 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    78781 agttttattg atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact
    78841 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    78901 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    78961 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca ttaagatgat
    79021 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    79081 gccgttggct attccgttag tcgccgggcc gactattctc gccacgctga tgttgctgtc
    79141 tcaccagtac ccaaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    79201 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    79261 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
    79321 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agaacccggt
    79381 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    79441 gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    79501 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac
    79561 aacaatcgaa ccaccagcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt
    79621 cagttgttca ataaccggca tcaccagtcc taccgccgtt aaacaggcta cggtggcaga
    79681 accctgaatg atgcgcactg cagcggccag cacgaagcag gtgatggcta tcggcaggcc
    79741 catgccagtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    79801 gaacacgcca cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    79861 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    79921 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    79981 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    80041 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttgcctt cgccgagatg
    80101 cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga aattacccca
    80161 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    80221 gccaaaatcg gcgttcatct gcgatgccag cagcattggc gctggccctg gcaccaggaa
    80281 cgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    80341 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    80401 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
    80461 gaaggattta agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
    80521 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    80581 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    80641 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    80701 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    80761 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    80821 caccagtaca tcagtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    80881 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacgtcaaa
    80941 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    81001 gtgttttttc aatgcagaac agacgatcag tgacacttta ttagtgcgct gcatagcaaa
    81061 cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca gcggttcgcc
    81121 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    81181 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    81241 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    81301 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga
    81361 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtaggctta
    81421 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    81481 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    81541 cagcacgctc gctaaccgcg gctccatcac ctggccaatg tcatgaccgt ggaaaccggc
    81601 aatcgccata tcgtcaggaa ccttcaatcc cagacgctga cattcaaacg ccgcgccgac
    81661 cgccaggtca tcgttggtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    81721 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatacgg
    81781 caccagacct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    81841 gtcgagacgc gcgccgagat aggcaatgtg gcaatgcccg cgagcaataa tggcggtggt
    81901 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    81961 gctgtccatg agttccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    82021 cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca tggattcgag
    82081 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct gataaccgtg
    82141 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    82201 aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg gcgcacgatt
    82261 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    82321 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    82381 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    82441 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    82501 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    82561 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    82621 aaaatgccgt ggagctttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    82681 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    82741 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
    82801 caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt tccatatcct
    82861 gatgcacttt caatgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgta
    82921 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca ggcatgatcc
    82981 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    83041 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    83101 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    83161 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    83221 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    83281 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    83341 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    83401 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttaatt
    83461 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    83521 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    83581 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    83641 gccctcgtca ttgacatact ccagcacacc gaccgaatta tccgctgcga atcccggttc
    83701 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    83761 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    83821 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    83881 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    83941 aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct gcggcagccc
    84001 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc
    84061 tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt caaagcgacg
    84121 attctgatac ggcgtgacgg tcagcccttt gcttttcgcc aacgcaaaca gctctttcgc
    84181 ctgcgcaagt gtcggagtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    84241 gcgcttcgcg tactcgaagt ggctgtccgc gtgggtgcag acaacaacca gcttaacatc
    84301 gggatcgttt agtacttcgt cgagatcgct ggtgaaatgg atatgggaat aaatgggagc
    84361 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    84421 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    84481 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    84541 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    84601 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
    84661 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa taataatcat
    84721 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
    84781 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
    84841 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
    84901 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
    84961 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
    85021 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
    85081 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
    85141 tgtgcgacaa ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc gataacgccc
    85201 cggcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt aagaagtacg
    85261 cattgcgtaa tggtgaatat gtcgatgcat attatatggc gcgggtgaag taagatagtg
    85321 ccctttttct gagatggaaa aagggtgtca ttcaaaatcg acataccttc ctttaaggta
    85381 tttatttgcc caatacatat attgagattt aactcataat tacttcctaa agtgtaatat
    85441 tttatttttt aatatatacg cctacaattt cctggagtaa ataaataaca attaacaagc
    85501 ataatattgc cattgataaa atagcatgcc ataaaaggac ttttcaggga tgagtaatat
    85561 tgtttacctg acagtaacgg gagaacaaca aggaagcatc tccgcaggtt gtgggacttc
    85621 tgagtctaca ggtaatcgtt ggcagagcgg gcatgaggat gaaatattta cattctcact
    85681 cttaaataat attaataata cggggcttgg ttcacagttc catggtataa c
//
LOCUS       DAZFDP010000004        83913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFDP010000004 DAZFDP010000000
VERSION     DAZFDP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            451..900
                     /gene="alaE"
                     /locus_tag="ACHJ1G_000299"
     CDS             451..900
                     /gene="alaE"
                     /locus_tag="ACHJ1G_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4762218.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(937..1281)
                     /gene="ygaC"
                     /locus_tag="ACHJ1G_000300"
     CDS             complement(937..1281)
                     /gene="ygaC"
                     /locus_tag="ACHJ1G_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4762219.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1433..1762
                     /gene="ygaM"
                     /locus_tag="ACHJ1G_000301"
     CDS             1433..1762
                     /gene="ygaM"
                     /locus_tag="ACHJ1G_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4762220.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2011..2256
                     /gene="nrdH"
                     /locus_tag="ACHJ1G_000302"
     CDS             2011..2256
                     /gene="nrdH"
                     /locus_tag="ACHJ1G_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4762221.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2253..2663
                     /gene="nrdI"
                     /locus_tag="ACHJ1G_000303"
     CDS             2253..2663
                     /gene="nrdI"
                     /locus_tag="ACHJ1G_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4762222.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2636..4780
                     /gene="nrdE"
                     /locus_tag="ACHJ1G_000304"
     CDS             2636..4780
                     /gene="nrdE"
                     /locus_tag="ACHJ1G_000304"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4762223.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4790..5749
                     /gene="nrdF"
                     /locus_tag="ACHJ1G_000305"
     CDS             4790..5749
                     /gene="nrdF"
                     /locus_tag="ACHJ1G_000305"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4762224.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6104..7306
                     /gene="proV"
                     /locus_tag="ACHJ1G_000306"
     CDS             6104..7306
                     /gene="proV"
                     /locus_tag="ACHJ1G_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4762225.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7299..8363
                     /gene="proW"
                     /locus_tag="ACHJ1G_000307"
     CDS             7299..8363
                     /gene="proW"
                     /locus_tag="ACHJ1G_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4762226.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8421..9413
                     /gene="proX"
                     /locus_tag="ACHJ1G_000308"
     CDS             8421..9413
                     /gene="proX"
                     /locus_tag="ACHJ1G_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4762227.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9605..10789
                     /locus_tag="ACHJ1G_000309"
     CDS             9605..10789
                     /locus_tag="ACHJ1G_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4762228.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10913..11650
                     /gene="ygaZ"
                     /locus_tag="ACHJ1G_000310"
     CDS             10913..11650
                     /gene="ygaZ"
                     /locus_tag="ACHJ1G_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4762229.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11640..11975
                     /gene="ygaH"
                     /locus_tag="ACHJ1G_000311"
     CDS             11640..11975
                     /gene="ygaH"
                     /locus_tag="ACHJ1G_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4762230.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12066..12596
                     /gene="emrR"
                     /locus_tag="ACHJ1G_000312"
     CDS             12066..12596
                     /gene="emrR"
                     /locus_tag="ACHJ1G_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4762231.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12723..13895
                     /gene="emrA"
                     /locus_tag="ACHJ1G_000313"
     CDS             12723..13895
                     /gene="emrA"
                     /locus_tag="ACHJ1G_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4762232.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13912..15450
                     /gene="emrB"
                     /locus_tag="ACHJ1G_000314"
     CDS             13912..15450
                     /gene="emrB"
                     /locus_tag="ACHJ1G_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4762233.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15514..16029)
                     /gene="luxS"
                     /locus_tag="ACHJ1G_000315"
     CDS             complement(15514..16029)
                     /gene="luxS"
                     /locus_tag="ACHJ1G_000315"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4762234.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16179..17735)
                     /gene="gshA"
                     /locus_tag="ACHJ1G_000316"
     CDS             complement(16179..17735)
                     /gene="gshA"
                     /locus_tag="ACHJ1G_000316"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4762235.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17808..18236)
                     /gene="yqaA"
                     /locus_tag="ACHJ1G_000317"
     CDS             complement(17808..18236)
                     /gene="yqaA"
                     /locus_tag="ACHJ1G_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4762236.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18233..18799)
                     /gene="yqaB"
                     /locus_tag="ACHJ1G_000318"
     CDS             complement(18233..18799)
                     /gene="yqaB"
                     /locus_tag="ACHJ1G_000318"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4762237.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19082..19158)
                     /locus_tag="ACHJ1G_000319"
     tRNA            complement(19082..19158)
                     /locus_tag="ACHJ1G_000319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19122..19124),aa:Arg,seq:acg)
     gene            complement(19222..19298)
                     /locus_tag="ACHJ1G_000320"
     tRNA            complement(19222..19298)
                     /locus_tag="ACHJ1G_000320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19262..19264),aa:Arg,seq:acg)
     gene            complement(19496..19572)
                     /locus_tag="ACHJ1G_000321"
     tRNA            complement(19496..19572)
                     /locus_tag="ACHJ1G_000321"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19536..19538),aa:Arg,seq:acg)
     gene            complement(19576..19668)
                     /locus_tag="ACHJ1G_000322"
     tRNA            complement(19576..19668)
                     /locus_tag="ACHJ1G_000322"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19632..19634),aa:Ser,seq:gct)
     gene            complement(19984..20169)
                     /gene="csrA"
                     /locus_tag="ACHJ1G_000323"
     CDS             complement(19984..20169)
                     /gene="csrA"
                     /locus_tag="ACHJ1G_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4762238.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20404..23034)
                     /gene="alaS"
                     /locus_tag="ACHJ1G_000324"
     CDS             complement(20404..23034)
                     /gene="alaS"
                     /locus_tag="ACHJ1G_000324"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4762239.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23162..23662)
                     /gene="recX"
                     /locus_tag="ACHJ1G_000325"
     CDS             complement(23162..23662)
                     /gene="recX"
                     /locus_tag="ACHJ1G_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4762240.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23905..24966)
                     /gene="recA"
                     /locus_tag="ACHJ1G_000326"
     CDS             complement(23905..24966)
                     /gene="recA"
                     /locus_tag="ACHJ1G_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4762241.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25046..25543)
                     /gene="pncC"
                     /locus_tag="ACHJ1G_000327"
     CDS             complement(25046..25543)
                     /gene="pncC"
                     /locus_tag="ACHJ1G_000327"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4762242.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25688..26773)
                     /gene="mltB"
                     /locus_tag="ACHJ1G_000328"
     CDS             complement(25688..26773)
                     /gene="mltB"
                     /locus_tag="ACHJ1G_000328"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4762243.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27029..27592
                     /gene="srlA"
                     /locus_tag="ACHJ1G_000329"
                     /gene_synonym="gutA"
     CDS             27029..27592
                     /gene="srlA"
                     /locus_tag="ACHJ1G_000329"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO4762244.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27589..28548
                     /gene="srlE"
                     /locus_tag="ACHJ1G_000330"
                     /gene_synonym="gutE"
     CDS             27589..28548
                     /gene="srlE"
                     /locus_tag="ACHJ1G_000330"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO4762245.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28559..28930
                     /gene="srlB"
                     /locus_tag="ACHJ1G_000331"
                     /gene_synonym="gutB"
     CDS             28559..28930
                     /gene="srlB"
                     /locus_tag="ACHJ1G_000331"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4762246.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28934..29713
                     /gene="srlD"
                     /locus_tag="ACHJ1G_000332"
     CDS             28934..29713
                     /gene="srlD"
                     /locus_tag="ACHJ1G_000332"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4762247.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29818..30177
                     /gene="gutM"
                     /locus_tag="ACHJ1G_000333"
     CDS             29818..30177
                     /gene="gutM"
                     /locus_tag="ACHJ1G_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4762248.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30244..31017
                     /gene="srlR"
                     /locus_tag="ACHJ1G_000334"
                     /gene_synonym="gutR"
     CDS             30244..31017
                     /gene="srlR"
                     /locus_tag="ACHJ1G_000334"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4762249.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31010..31975
                     /gene="gutQ"
                     /locus_tag="ACHJ1G_000335"
     CDS             31010..31975
                     /gene="gutQ"
                     /locus_tag="ACHJ1G_000335"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4762250.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31972..33486)
                     /gene="norR"
                     /locus_tag="ACHJ1G_000336"
     CDS             complement(31972..33486)
                     /gene="norR"
                     /locus_tag="ACHJ1G_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4762251.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            33673..35112
                     /gene="norV"
                     /locus_tag="ACHJ1G_000337"
     CDS             33673..35112
                     /gene="norV"
                     /locus_tag="ACHJ1G_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4762252.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            35109..36242
                     /gene="norW"
                     /locus_tag="ACHJ1G_000338"
     CDS             35109..36242
                     /gene="norW"
                     /locus_tag="ACHJ1G_000338"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4762253.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36468..38720)
                     /gene="hypF"
                     /locus_tag="ACHJ1G_000339"
     CDS             complement(36468..38720)
                     /gene="hypF"
                     /locus_tag="ACHJ1G_000339"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4762254.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38873..39400)
                     /gene="hydN"
                     /locus_tag="ACHJ1G_000340"
     CDS             complement(38873..39400)
                     /gene="hydN"
                     /locus_tag="ACHJ1G_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4762255.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39549..40559)
                     /gene="ascG"
                     /locus_tag="ACHJ1G_000341"
     CDS             complement(39549..40559)
                     /gene="ascG"
                     /locus_tag="ACHJ1G_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4762256.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            40819..42276
                     /gene="ascF"
                     /locus_tag="ACHJ1G_000342"
     CDS             40819..42276
                     /gene="ascF"
                     /locus_tag="ACHJ1G_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO4762257.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            42285..43709
                     /gene="ascB"
                     /locus_tag="ACHJ1G_000343"
     CDS             42285..43709
                     /gene="ascB"
                     /locus_tag="ACHJ1G_000343"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4762258.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43823..44242)
                     /locus_tag="ACHJ1G_000344"
     CDS             complement(43823..44242)
                     /locus_tag="ACHJ1G_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4762259.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(44239..44550)
                     /locus_tag="ACHJ1G_000345"
     CDS             complement(44239..44550)
                     /locus_tag="ACHJ1G_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4762260.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(44724..45467)
                     /locus_tag="ACHJ1G_000346"
     CDS             complement(44724..45467)
                     /locus_tag="ACHJ1G_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762261.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(45510..45980)
                     /gene="hycI"
                     /locus_tag="ACHJ1G_000347"
     CDS             complement(45510..45980)
                     /gene="hycI"
                     /locus_tag="ACHJ1G_000347"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4762262.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45973..46383)
                     /gene="hycH"
                     /locus_tag="ACHJ1G_000348"
     CDS             complement(45973..46383)
                     /gene="hycH"
                     /locus_tag="ACHJ1G_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4762263.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46380..47147)
                     /gene="hycG"
                     /locus_tag="ACHJ1G_000349"
     CDS             complement(46380..47147)
                     /gene="hycG"
                     /locus_tag="ACHJ1G_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4762264.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47147..47689)
                     /gene="hycF"
                     /locus_tag="ACHJ1G_000350"
     CDS             complement(47147..47689)
                     /gene="hycF"
                     /locus_tag="ACHJ1G_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4762265.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47699..49408)
                     /gene="hycE"
                     /locus_tag="ACHJ1G_000351"
     CDS             complement(47699..49408)
                     /gene="hycE"
                     /locus_tag="ACHJ1G_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4762266.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49426..50349)
                     /gene="hycD"
                     /locus_tag="ACHJ1G_000352"
     CDS             complement(49426..50349)
                     /gene="hycD"
                     /locus_tag="ACHJ1G_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4762267.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50352..52178)
                     /gene="hycC"
                     /locus_tag="ACHJ1G_000353"
     CDS             complement(50352..52178)
                     /gene="hycC"
                     /locus_tag="ACHJ1G_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4762268.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52175..52786)
                     /gene="hycB"
                     /locus_tag="ACHJ1G_000354"
     CDS             complement(52175..52786)
                     /gene="hycB"
                     /locus_tag="ACHJ1G_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4762269.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52911..53372)
                     /gene="hycA"
                     /locus_tag="ACHJ1G_000355"
     CDS             complement(52911..53372)
                     /gene="hycA"
                     /locus_tag="ACHJ1G_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4762270.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53584..53934
                     /gene="hypA"
                     /locus_tag="ACHJ1G_000356"
     CDS             53584..53934
                     /gene="hypA"
                     /locus_tag="ACHJ1G_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4762271.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53938..54810
                     /gene="hypB"
                     /locus_tag="ACHJ1G_000357"
     CDS             53938..54810
                     /gene="hypB"
                     /locus_tag="ACHJ1G_000357"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4762272.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54801..55073
                     /gene="hypC"
                     /locus_tag="ACHJ1G_000358"
     CDS             54801..55073
                     /gene="hypC"
                     /locus_tag="ACHJ1G_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4762273.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55073..56194
                     /gene="hypD"
                     /locus_tag="ACHJ1G_000359"
     CDS             55073..56194
                     /gene="hypD"
                     /locus_tag="ACHJ1G_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4762274.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56191..57201
                     /gene="hypE"
                     /locus_tag="ACHJ1G_000360"
     CDS             56191..57201
                     /gene="hypE"
                     /locus_tag="ACHJ1G_000360"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4762275.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57275..59343
                     /gene="flhA"
                     /locus_tag="ACHJ1G_000361"
                     /pseudo
     CDS             57275..59343
                     /gene="flhA"
                     /locus_tag="ACHJ1G_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(59380..59733)
                     /gene="ygbA"
                     /locus_tag="ACHJ1G_000362"
     CDS             complement(59380..59733)
                     /gene="ygbA"
                     /locus_tag="ACHJ1G_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4762276.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(59810..59944)
                     /locus_tag="ACHJ1G_000363"
     CDS             complement(59810..59944)
                     /locus_tag="ACHJ1G_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762277.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            60020..62581
                     /gene="mutS"
                     /locus_tag="ACHJ1G_000364"
     CDS             60020..62581
                     /gene="mutS"
                     /locus_tag="ACHJ1G_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4762278.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62687..63343
                     /gene="pphB"
                     /locus_tag="ACHJ1G_000365"
     CDS             62687..63343
                     /gene="pphB"
                     /locus_tag="ACHJ1G_000365"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4762279.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(63394..64161)
                     /gene="ygbI"
                     /locus_tag="ACHJ1G_000366"
     CDS             complement(63394..64161)
                     /gene="ygbI"
                     /locus_tag="ACHJ1G_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO4762280.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            64357..65265
                     /gene="ltnD"
                     /locus_tag="ACHJ1G_000367"
     CDS             64357..65265
                     /gene="ltnD"
                     /locus_tag="ACHJ1G_000367"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO4762281.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            65262..66524
                     /gene="otnK"
                     /locus_tag="ACHJ1G_000368"
     CDS             65262..66524
                     /gene="otnK"
                     /locus_tag="ACHJ1G_000368"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO4762282.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            66521..67159
                     /gene="ygbL"
                     /locus_tag="ACHJ1G_000369"
     CDS             66521..67159
                     /gene="ygbL"
                     /locus_tag="ACHJ1G_000369"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO4762283.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            67164..67940
                     /gene="ygbM"
                     /locus_tag="ACHJ1G_000370"
     CDS             67164..67940
                     /gene="ygbM"
                     /locus_tag="ACHJ1G_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4762284.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            68029..69393
                     /gene="ygbN"
                     /locus_tag="ACHJ1G_000371"
     CDS             68029..69393
                     /gene="ygbN"
                     /locus_tag="ACHJ1G_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO4762285.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(69432..69668)
                     /locus_tag="ACHJ1G_000372"
     CDS             complement(69432..69668)
                     /locus_tag="ACHJ1G_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4762286.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(69679..71106)
                     /locus_tag="ACHJ1G_000373"
     CDS             complement(69679..71106)
                     /locus_tag="ACHJ1G_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4762287.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(71106..71699)
                     /locus_tag="ACHJ1G_000374"
     CDS             complement(71106..71699)
                     /locus_tag="ACHJ1G_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4762288.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            71846..72253
                     /locus_tag="ACHJ1G_000375"
     CDS             71846..72253
                     /locus_tag="ACHJ1G_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4762289.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(72374..73366)
                     /gene="rpoS"
                     /locus_tag="ACHJ1G_000376"
     CDS             complement(72374..73366)
                     /gene="rpoS"
                     /locus_tag="ACHJ1G_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4762290.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(73429..74523)
                     /gene="nlpD"
                     /locus_tag="ACHJ1G_000377"
     CDS             complement(73429..74523)
                     /gene="nlpD"
                     /locus_tag="ACHJ1G_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4762291.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(74708..75334)
                     /gene="pcm"
                     /locus_tag="ACHJ1G_000378"
     CDS             complement(74708..75334)
                     /gene="pcm"
                     /locus_tag="ACHJ1G_000378"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4762292.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(75328..76089)
                     /gene="surE"
                     /locus_tag="ACHJ1G_000379"
     CDS             complement(75328..76089)
                     /gene="surE"
                     /locus_tag="ACHJ1G_000379"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4762293.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(76070..77119)
                     /gene="truD"
                     /locus_tag="ACHJ1G_000380"
     CDS             complement(76070..77119)
                     /gene="truD"
                     /locus_tag="ACHJ1G_000380"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4762294.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(77116..77595)
                     /gene="ispF"
                     /locus_tag="ACHJ1G_000381"
     CDS             complement(77116..77595)
                     /gene="ispF"
                     /locus_tag="ACHJ1G_000381"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4762295.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(77595..78305)
                     /gene="ispD"
                     /locus_tag="ACHJ1G_000382"
     CDS             complement(77595..78305)
                     /gene="ispD"
                     /locus_tag="ACHJ1G_000382"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4762296.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(78324..78635)
                     /gene="ftsB"
                     /locus_tag="ACHJ1G_000383"
     CDS             complement(78324..78635)
                     /gene="ftsB"
                     /locus_tag="ACHJ1G_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4762297.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(78829..79152)
                     /gene="ygbE"
                     /locus_tag="ACHJ1G_000384"
     CDS             complement(78829..79152)
                     /gene="ygbE"
                     /locus_tag="ACHJ1G_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4762298.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(79202..79807)
                     /gene="cysC"
                     /locus_tag="ACHJ1G_000385"
     CDS             complement(79202..79807)
                     /gene="cysC"
                     /locus_tag="ACHJ1G_000385"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4762299.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(79807..81234)
                     /gene="cysN"
                     /locus_tag="ACHJ1G_000386"
     CDS             complement(79807..81234)
                     /gene="cysN"
                     /locus_tag="ACHJ1G_000386"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4762300.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(81236..82144)
                     /gene="cysD"
                     /locus_tag="ACHJ1G_000387"
     CDS             complement(81236..82144)
                     /gene="cysD"
                     /locus_tag="ACHJ1G_000387"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4762301.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            82396..83433
                     /gene="iap"
                     /locus_tag="ACHJ1G_000388"
     CDS             82396..83433
                     /gene="iap"
                     /locus_tag="ACHJ1G_000388"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4762302.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   83515..83909
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=83576..83604
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT        19790 a        22655 c        21612 g        19856 t
ORIGIN      
        1 tcgctaattc cgcaagcctg acaaaaccga agtgaataaa aatgcaatat aacagaataa
       61 aagaaaacac ccctcactgg tgctttctgc tgttaatgta cattttctgg cataaaaaat
      121 gctgtaaatg acccggtcac gacatacacc gaattctcta cgcttatagc gttacctcac
      181 ccccaaactt tcattagtga aattaaaggc aaggtcatat ccaattgatt taattgaaaa
      241 taaatcatat cggcatgtaa aaatccacag tatacaaaaa acgagccgtt acggaatatt
      301 ttatctacaa aaactgacta aataaaaatt tttcactaat tgaatagtca tagccagcga
      361 tatacgctat gcgaaaatgc agatggcaat gagatccact gctttcatct ccattaacat
      421 cccattacgc ttttattaag gagcattagc atgttctcac cgcagtcacg cttgcgtcat
      481 gcagttgcag atacgttcgc gatggttgtt tactgttctg tcgtgaacat gtgtattgaa
      541 gttttcctct ccggaatgag cttcgaacag tctttttatt ccagattggt agcgattccg
      601 gcgaacatct taattgcatg gccatacggt atgtaccgcg atctgtttat gcgcgcggca
      661 cgcaaagtta gcccgtcggg ctggataaaa aatctggctg atatcctggc ttatgtgacg
      721 ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg gcatcagatt
      781 atggcggcgg tcagttcaaa catcgttgtt tcgatgttga tgggggcggt ttatggctac
      841 ttcctcgatt attgccgccg actgtttaaa gtcagccgtt accagcaggt aaaagcctga
      901 ctcttctttt cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc caaataaacc
      961 attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac cgtaatgctc
     1021 gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc
     1081 ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat gttggatgaa ttaccagagc
     1141 ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac ggcggtaacc
     1201 atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta cgcgcgccac
     1261 ctcgtctggt cgtaaataca tatttgatcc tcgttattat caaccgcggg cctaccttac
     1321 ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga acctcagtta
     1381 agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc atatgtttaa
     1441 ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg atgtcaatca
     1501 attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca aaggggaagc
     1561 tgaagccgca cgcagtaaag ctcaggcatt gctgaaagaa accagggcgc gaatgcatgg
     1621 tcgtactcgc gtccagcaag ccgcacgcga tgccgttggc tgcgcggatt cttttgttcg
     1681 tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta tcggcgcact
     1741 gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt tgaccccgaa
     1801 gcctggcttc ggggtttctt tttgcctggt gaatcacaaa aatcccccta ccccgtcacg
     1861 ctcatatcca gggtaatttc gaccactatt tgctatatat tgtgtggttg aatctttttt
     1921 tcaactacat ctagtatctc tgtatcaaca gagagacaac ccgacgcgta tcatcgcgcc
     1981 gtatcttcat tttaaacgga aatacgaatc atgcgcatta ctatttacac tcgtaacgat
     2041 tgcgttcagt gccacgccac caaacgggcg atggaaaacc ggggctttga ttttgaaatg
     2101 attaatgtcg atcgcgttcc tgaagcagcg gaagcgttgc gtgctcaggg ctttcgtcag
     2161 ttgccggtag tgattgctgg cgatcttagc tggtctggtt tccgtccgga catgattaac
     2221 cgtctgcatc cagcgccaca cgcggccagt gcatgagcca gctcgtctac ttctccagca
     2281 gctccgaaaa cacgcagcgt tttatcgaac gtttaggtct gcccgcggtg cgcatcccgc
     2341 tcaatgagcg ggaacggatt caggtagacg agccttacat cctgatcgtg ccctcttacg
     2401 gcggcggcgg tacggctggc gcggtgccac gacaggtaat tcgcttttta aacgacgagc
     2461 acaaccgggc gttgcttcgc ggcgttattg cttctggtaa tcgcaacttt ggtgaggcgt
     2521 atggccgcgc cggagatgtg attgcccgga aatgcggcgt gccgtggctg taccgttttg
     2581 aactcatggg tacgcaaagc gatatcgaaa acgttcgtaa aggagtaacc gaattttggc
     2641 aacgacaacc gcagaatgcc tgacgcagga aacgatggat taccacgcgc tgaatgcgat
     2701 gcttaacctc tacgatagcg caggtcgcat tcagttcgat aaagaccgcc aggccgttga
     2761 cgcctttatt gcgacgcatg tgcgtccgaa cagtgtgacc ttcagtagcc agcagcagcg
     2821 cctgaactgg ctggtcaacg aaggttacta tgatgaaagc gttcttaatc gctactctcg
     2881 cgactttgtc attacgctgt ttgcccacgc acacaccagc ggttttcgtt tccagacatt
     2941 cctcggggca tggaagtttt acaccagcta tacgttgaag acattcgacg gtaaacgtta
     3001 tctggaagat tttgccgatc gagtcacgat ggtggcgctg acgctggcac aaggcgatgc
     3061 gacgctggcg ttgcaactga ccgatgaaat gctgtcagga cgctttcagc cagccacgcc
     3121 aacattcctc aactgcggta agcagcagcg cggcgaactg gtttcctgtt ttctgctgcg
     3181 tattgaagac aatatggagt cgattggccg ggcggtaaat tccgcactgc aactgtcgaa
     3241 acgcggcggc ggcgtggcat ttttgctgtc gaatctgcgg gaagcgggcg cgccaattaa
     3301 acgtattgaa aatcaatctt ctggcgtaat tccggtgatg aaaatgctgg aagacgcatt
     3361 ttcctatccc aaccaactcg gcgcgcgtca gggggctggt gcagtctatt tacatgctca
     3421 tcatcccgat attctgcgtt ttctcgacac gaaacgggaa aatgccgacg aaaaaatccg
     3481 cattaaaaca ctgtcgcttg gcgtggtgat cccggatatc actttccatc tggcaaaaga
     3541 gaatgcgcag atggcgctgt tttcgcccta tgacgttgag cgagtttatg gcaagccgtt
     3601 tgccgatatc gccattagcg aacactatga cgaactggtt gccgatgaac gcatccgcaa
     3661 aaaatacctc aacgcccgtg atttcttcca gcgactggca gaaatccagt ttgagtccgg
     3721 ctatccctac atcatgtatg aagacacggt aaaccgtgct aaccctatcg ccgggcgcat
     3781 aaatatgagt aatctctgct cagaaatttt gcaggttaac agcgcctcag agtatgacga
     3841 gaatctcgac tatgcccgta caggccatga tatttcctgc aatttaggtt cgttgaatat
     3901 tgcccacacc atggattccc ccaactttgc ccgcacggta gagactgccg tacgcggttt
     3961 aacggcggtg tcagatatga gtcatatccg cagcgtgccg tccatcgaag ccggaaatgc
     4021 cgcctcgcac gccatcggac tggggcagat gaatttacat ggttatctgg cgagggaagg
     4081 catcgcttat ggttcgccgg aagccctgga tttcaccaat ctctatttct ataccatcac
     4141 ctggcacgca ctgcgtacct cgatgttgct ggcgcgcgaa cgcggtgaaa ccttcgccgg
     4201 gttcaaacag tcacgctatg ccagtggtga atatttcagc caatatctgc aaggtaactg
     4261 gcagccgaaa acggcgaaag ttggcgaact gtttgcccgc agcggtatta cgttacctac
     4321 ccgtgagatg tgggcgcaac tgcgcgacga cgtgatgcgc tacggcatat acaaccagaa
     4381 tctccaggcg gtgccgccaa ccggttctat ctcttatatc aaccatgcta cgtcgagtat
     4441 tcatccgatt gtggcgaaag tagagatacg caaagagggc aaaacaggac gcgtttacta
     4501 ccctgccccg tttatgacta acgagaatct ggcgctgtat caggatgctt acgaaatcgg
     4561 cgcagaaaag atcatcgaca cctacgcgga agcgactcgc catgtcgatc agggtttgtc
     4621 gctgacgctg tttttccccg ataccgccac cactcgcgat atcaacaaag cgcagattta
     4681 cgcctggcgc aaaggtatca aaacgctcta ttacattcgc ctgcgtcaga tggcgctgga
     4741 aggcactgaa attgaaggct gcgtctcctg tgcactttaa ggaatatcta tgaaactctc
     4801 acgtatcagc gccatcaact ggaacaagat atctgacgat aaagatctgg aggtgtggaa
     4861 tcgcctgacc agcaatttct ggctaccaga aaaggtgcca ctgtcgaacg atatacctgc
     4921 atggcagaca ttaagtgtcg tagaacaaca actgacaatg cgcgttttta ctggcctgac
     4981 gctgctcgac acgctgcaaa atgttatcgg cgcgccttcg ctgatgcccg atgcgctcac
     5041 gcctcatgaa gaggcagtat tatcgaatat cagctttatg gaagcggttc atgcccgctc
     5101 ttacagttcg attttctcga cgctgtgtca gaccaaagat gtcgatgccg cctacgcctg
     5161 gagtgaagaa aacgcaccgt tgcagcgaaa agctcagatt attcagcaac attatcgcgg
     5221 tgatgatccg ctgaaaaaga aaatcgccag tgtgtttctt gaatcttttt tgttctattc
     5281 cggtttctgg ctgccgatgt atttttccag ccgcggaaag ctgaccaata ccgcagatct
     5341 gatccgtctg attatccgcg atgaagcagt ccacggttac tacataggct ataaatatca
     5401 gaaaaacatg gaaaagatat ctctgggaca acgtgaagag ttgaagagtt tcgccttcga
     5461 tttgttgctg gaactctacg acaacgagtt gcaatacacc gatgagctgt acgccgaaac
     5521 cccgtgggct gacgatgtga aagcgtttct ctgttacaac gccaataagg ctttgatgaa
     5581 tctgggctac gaaccgttat ttcccgcaga aatggcggaa gtgaatccgg caatcctcgc
     5641 cgcgctttcg ccgaatgccg atgaaaatca cgatttcttt tccggttcag gctcctctta
     5701 tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag ggtgttattt
     5761 tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt tttatcacgc
     5821 aaatattctg tagtgatcta cactgatact ctgttgcatt attcgcctga aactacaata
     5881 ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat ttgctctcag
     5941 cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc cacatttgcc
     6001 ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact atttccattg
     6061 ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa ttaaattaga
     6121 aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca aatatatcga
     6181 acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg gcgtaaaaga
     6241 cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg
     6301 taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtgct
     6361 gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg tgcgcagaaa
     6421 aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa
     6481 tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct
     6541 tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg atgaactctc
     6601 tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatatt
     6661 attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga
     6721 gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc acgatctcga
     6781 tgaggctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt
     6841 cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta ccttcttccg
     6901 tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga caccgaatgg
     6961 cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat tgcaggatga
     7021 agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtttccat
     7081 cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc
     7141 gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc
     7201 accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg
     7261 aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt
     7321 gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg ggtacaccgg
     7381 cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg
     7441 tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg
     7501 tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag ggcgtgcgcg
     7561 ttccggttga ttacattctc aacggtttcc agcaattgct gctgggtatg cccgcaccgg
     7621 tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcgtgg
     7681 cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag gccatggtta
     7741 ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg ccgctgggga
     7801 tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt gatgccatgc
     7861 agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg
     7921 tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt ctgaccattc
     7981 tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc
     8041 cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgaccatt atggcgggcg
     8101 ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg attgccgtcg
     8161 gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg cttgccaccg
     8221 ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg caggccgttg
     8281 ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct gttggtctgc
     8341 tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc attatcaccc
     8401 tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc
     8461 ttatctctac acaaactttt gctgccgatc tgccgggcaa aggcattact gttaatccag
     8521 ttcagagcac catcactgaa gaaaccttcc agacgctgct ggtcagtcgt gcgctggaga
     8581 aattaggtta taccgtcaac aaacccagcg aagtggatta caacgttggc tacacctcgc
     8641 ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt
     8701 acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg
     8761 cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc aacatcgcac
     8821 aactgaaaga tccgaagatc gccaaactgt tcgataccaa cggcgacgga aaagcggatt
     8881 taaccggttg taaccctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt
     8941 atgaactgac ccataccgtg acgcataatc aggggaacta cgcggcgatg atggccgaca
     9001 ccatcagtcg ctacaaagag ggcaaaccgg tgttttacta cacctggacg ccgtactggg
     9061 tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg ttctccgcac
     9121 tgccgggcga taaaaacgcc gataccaaac tgccgaatgg tgcgaattat ggcttcccgg
     9181 tcagcaccat gcatatcgtt gccaacaaag cctgggccga gaaaaacccg gcagcagcga
     9241 aactgtttgc cattatgcag ttgccagtgg cagatattaa cgcccagaac gccattatgc
     9301 atgacggcaa agcctcagaa ggcgatattc agggccatgt tgatggctgg atcaaagccc
     9361 accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgcagaag taatttttat
     9421 tcgggcggat aaggcgtttt tgcctcatcc gccgctctgt acacaatgcc tgatgcgacg
     9481 ctgacgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc
     9541 ccatattcat caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa
     9601 aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc
     9661 caccggtctg gcggtcgcca gtaactatta cgcccagccg ctactcgaca ccatcgcgcg
     9721 taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta
     9781 tgcagcaggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat
     9841 tgtctcgatg accttactgg cagcgggcgg tatgttgatt accgccagca gtcagtcgct
     9901 ggcgatgatg ctcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct
     9961 ggttccgctg gcagcgacgc tggcttcacc ggataaacgc ggcaaagtgg ttggcaccat
    10021 tatgagcggt ctgctgttgg ggatattgct ggcacgaacc gttgccgggt tactggcaaa
    10081 cctcggcggc tggcgcaccg tcttttgggt tgcctcggtg ttaatggctc tgatggcgct
    10141 agcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt
    10201 gttgggttcc gttttcagca tgtttatcaa cgataagatc ctgcgcaccc gcgcattgct
    10261 tggctgcctg acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc
    10321 cgctccacct tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc
    10381 cggcgcgttg ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac
    10441 cacaactttc ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac
    10501 ttccgtactg gcgttgatta tcggaatcct ggtgctggat ctcaccgtac agggcgtgca
    10561 tatcactaac cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc
    10621 aggttacatg accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc
    10681 ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa
    10741 cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa
    10801 ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta
    10861 agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag
    10921 ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca aagacagttt
    10981 accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct
    11041 gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca
    11101 gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt
    11161 catggcaatg gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca
    11221 gcgtctgcaa aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc
    11281 cgccgcaacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg
    11341 cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg
    11401 cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc
    11461 actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc
    11521 agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc
    11581 aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga
    11641 tgagctatga ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct
    11701 atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta
    11761 ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac
    11821 cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg
    11881 gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct
    11941 atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac
    12001 aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa tgaggtcata
    12061 cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc
    12121 acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca
    12181 agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta
    12241 tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt
    12301 gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg
    12361 gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg
    12421 aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac
    12481 tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc
    12541 tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg
    12601 tcatctcgct caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat
    12661 gctggccttt ttggcaagca gctcggctca gccgatgagt taagaagatc gtggagaaca
    12721 atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac
    12781 gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga
    12841 tttattggtt tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga
    12901 atcagattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca
    12961 ccgattttgt aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg
    13021 cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga
    13081 ttaacagcaa acagttgcag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac
    13141 aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc
    13201 tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac
    13261 aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc
    13321 aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca
    13381 gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa
    13441 cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag
    13501 agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg
    13561 gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat
    13621 tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac
    13681 ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt
    13741 ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac
    13801 gttcaacacc ggtcgcggta agtaccgcgc gtgaaatcag cctggcacct gtcaataaac
    13861 tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag
    13921 caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca
    13981 ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat
    14041 ctgggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc
    14101 atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt
    14161 tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag cagcctgaat
    14221 atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat cccgctttcg
    14281 caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcgct ggcgttgtgg
    14341 tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta tatcagcgat
    14401 aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc ggtggtgttg
    14461 atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg gattgatgcc
    14521 gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct tgaccgcggt
    14581 aaagagctgg attggttttc atcacaggaa attatcatcc ttaccgtggt ggcggtggtg
    14641 gctatctgct tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg
    14701 ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc
    14761 tacttcggcg ctattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg
    14821 acctgggcag gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt
    14881 atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg
    14941 tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg
    15001 tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc
    15061 accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac
    15121 tttacgcgaa cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac
    15181 cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc
    15241 caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg
    15301 attgcccagc agatcaccaa tcaggggctg attatttccg ccaatgagat cttctggatg
    15361 tcagccggaa tattcctcgt actgctgggg ctggtgtggt ttgctaaacc gccatttggc
    15421 gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc
    15481 ttttttcaat taattgtgaa gatagtttac tgactagatg tgcagttcct gcaacttctc
    15541 tttcggcagt gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg
    15601 cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt
    15661 cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca
    15721 ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg
    15781 gcagcccatt ggcgagatat cgataatctc tacaccctta ccgttaagat ggttacgcat
    15841 aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca cttctttgtt
    15901 cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt
    15961 tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa
    16021 caacggcatt tagccacctc cggtaatttt ttttaaaatt ttctgaactc tttcttccca
    16081 ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg
    16141 aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac
    16201 accgcaaacg gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg
    16261 cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg cagcagatta
    16321 cggtaggctt ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt
    16381 aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag ttcatcacac
    16441 actttctgat acgcttcgcc gccgttaatg ctatccagcg tttgcgcgac gcgtttcaga
    16501 tcgcggaaca gatctttacc cacctgcagt aacgggaact gcgcggtttc gcagccgata
    16561 cccagcgtca ggcccggttt gcgaccttca aggatcaccc ggttccagtt aacgcgcgta
    16621 caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac
    16681 aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc
    16741 agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg
    16801 ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca gttcgttttc aatctgcagc
    16861 acgttgctat tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct
    16921 tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag
    16981 gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag
    17041 gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt
    17101 tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc
    17161 caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg
    17221 gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa
    17281 ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca
    17341 cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc
    17401 tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg
    17461 cgcgccgtat aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc
    17521 actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc
    17581 agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc agcattaaca
    17641 cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc
    17701 agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg
    17761 ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac
    17821 atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt accaaggcac
    17881 aaaaaaaaga ttactggccc ccaggagatg cgcatccatc ccgctaacag acacagtaaa
    17941 tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag
    18001 ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata
    18061 acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga
    18121 taactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt
    18181 gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca
    18241 agcgaacatc cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat
    18301 cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg
    18361 tgtctggcgc gggtttatgg tatttgacgt gatcagcagc gacgacggcg tcaaaataac
    18421 ggcgtaatcc caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta
    18481 cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga
    18541 cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggt
    18601 cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat
    18661 taagcgcaat cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc
    18721 acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac
    18781 ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt
    18841 aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta
    18901 gttatttaga aatgagatat ttttgaagga aatttttggg agaagatggt gcatccggga
    18961 ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt atcatccctg
    19021 gtgcctgcgg ttaattccgc tacctcttcg ataccttcat tgctgaagat ttcggagaat
    19081 atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc
    19141 cagctgagct acggatgcat cgggaaaatt tattttactg ctgatattga taccgctact
    19201 aaagccatat caagtaagag atggtgcatc cgggaggatt cgaacctccg accgctcggt
    19261 tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc
    19321 agattttttg ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat
    19381 tactcgcctt gcggctcgcc cttcgggccg ttgctaaagc aacgttatcc tccctggtgc
    19441 ctgcggttat accgccacct cttccatacc ttcatcgctg aaagtacgta gaatatggtg
    19501 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    19561 agctacggat gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg
    19621 catactccct tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta
    19681 cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt
    19741 ctgagactta taagtcaaac aatttttcct acacttttat cgtttgcaca cttcacgttc
    19801 aattagtctg taaaaacgtc aaaaagagtg ttttatcaac agaaaaatgg aggtctgaca
    19861 gatagtagta atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag
    19921 actatagagt gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa
    19981 agattagtaa ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg
    20041 gtgaacagaa acttccttcg gggcatttac gccaatacgt acctggttgc cctttacccc
    20101 taaaactgtc acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag
    20161 aatcagcatt ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat
    20221 tatccatcat ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat
    20281 ggcattacat tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat
    20341 cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt
    20401 atattattgc aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc
    20461 cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac
    20521 ctgctgagcg accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga
    20581 tacgcctgca atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga
    20641 ccccagctga ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct
    20701 aagctcgcta accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc
    20761 gctctcctgt gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt
    20821 acgttccagt actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg
    20881 cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt
    20941 taccgcttcg atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag
    21001 accaatatca ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc
    21061 catgctcagc acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt
    21121 cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg
    21181 tgcgttcacc aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga
    21241 gaagtcgaag cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc
    21301 cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg
    21361 ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc
    21421 agcaagttta ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa
    21481 ggagaagtta gcgcctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata
    21541 gaatggtgtt tgatccagca cgaccacagc ttcctggcct gcattgattg catcaaccgc
    21601 tttaccatca acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt
    21661 aaattcagat gcactatcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc
    21721 ttcgcgcgcg cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt
    21781 gatgttgcgc tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta
    21841 cagacggaaa gccgtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc
    21901 cagcaacgcc agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac
    21961 ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat
    22021 cagcggacca accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg
    22081 cactgcgcga cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac
    22141 gccatccgcg atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt
    22201 attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag
    22261 gtcgatgtca tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat
    22321 accggtatct acagacggct tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa
    22381 ctgcatgaag acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc
    22441 cggaggacca ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca
    22501 cggaccagtg tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc
    22561 gccgatgcga ataatacgtt cgcgcgggat cccgacttct ttttcccaga tttcgtaggc
    22621 ttcgtcgtcg ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt
    22681 ttcgctggtc agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa
    22741 gctgaagttg cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc
    22801 caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata
    22861 attacgcttg tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa
    22921 caacaaagtt gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt
    22981 actatggaaa aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat
    23041 cctgaaatca agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg
    23101 gtaactgaaa agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg
    23161 ttcagtcggc aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga
    23221 gcagaaaacg ctggatctta actttttctg aaaagacagt tggcaaaggt tcaccatatt
    23281 ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt
    23341 tttctgtcgc ttcgcgggaa atacctttct gattcagttc ctgacgaata cgtgcaggtc
    23401 cataaccttt gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat
    23461 agccatgttc atggcaccag gcaataacgc gctcgtaatc ttctgccgta gcatcaatct
    23521 cttctgggcc atttttgccc ataatcggtg ccacgagttt acgtcgcagt tcttgctcac
    23581 tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc
    23641 gacgggacgt tgattctgtc atggcatatc cttacaactt aaaaaagcaa aagggccgca
    23701 gatgcgaccc aggttttgct gataaacaga tttaatagtc aatgagtgtg cagaatgttc
    23761 ccgcaggccc aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgctggcc
    23821 tgttcccctc cccctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta
    23881 tctattatca tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct
    23941 gtcatctaca gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt
    24001 cttctcgatc tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt
    24061 accctgaccg atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt
    24121 ctcttttacg cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat
    24181 ctgaaattca gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt
    24241 ttcgctaccc accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg
    24301 aacagaggcg tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat
    24361 cacaccaatt ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag
    24421 gttacccgcc agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga
    24481 gtcgccgatt tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac
    24541 gataacgtct actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc
    24601 ggtgtccggc tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat
    24661 tgggtccagc gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc
    24721 ggcgatcacc tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac
    24781 gatacggccc atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt
    24841 agagatggtt tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc
    24901 aaattgtttc tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat
    24961 agccattttt actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc
    25021 aattatactg tatgctcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct
    25081 gcaatgcata cgcagtagcc tgacgacgca ccgcatcacg gtcaccgctg aagcattccc
    25141 gccgggtaat gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct
    25201 tctcttcact gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac
    25261 gagccgcttt cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat
    25321 gctgcgccag cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg
    25381 tgacaaatcc gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta
    25441 cccaaccacc ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct
    25501 gcccaacctg ttcacttaac tgcatcagtt cactgtcagt catcacaacc tctgtcagtc
    25561 aaaaatttat gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt
    25621 ttacgaggag gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg
    25681 caaatagtta ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg
    25741 cgtaatgggt gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt
    25801 actggtaacc ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg
    25861 gctgctgtgg cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc
    25921 catttggcaa gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc
    25981 agccgtgcgc tttgaaatag ttcgccacgc taccgatcgc atcaaccgga tcccacaggt
    26041 tgatatgccc gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact
    26101 gtacgtagcc catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt
    26161 cgcgcgccat caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt
    26221 agttaaatga cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc
    26281 agcgggtttc aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc
    26341 acgcgcgatt caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt
    26401 ccggcgtaat aaattttttg cgataacgga gccatgcgcc gttcggacct gatggcggtt
    26461 tcaccgatgt ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg
    26521 cctgggagag aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattttgt
    26581 cgatgaactg ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt
    26641 gctgcggctc aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag
    26701 gttttggctt gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac
    26761 gacgcttgaa catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca
    26821 tcacgcccca cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact
    26881 cattcccctc gctatccttt tactcaaact ttcaaattaa aatatttatc tttcattttg
    26941 agatcaaaat aacactttta aatctttcaa tctgattaga ttaggttgcc gtttggtaat
    27001 aaaacaataa atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg
    27061 gtttatcggg ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct
    27121 tccgctgttg attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca
    27181 tcgtattgaa cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctactgtt
    27241 accgtgcatt ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat
    27301 gccggaaaag tacaaaccca gctactacgc ggcggcctct tatagctgcc actcaatgaa
    27361 tggcctcttc ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg
    27421 tctgacaacg ctgaacctgc cacttggccc actggcggtg agttatctgc tggttggtct
    27481 ggtcaccaat ttcttccgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa
    27541 gatgggcatt caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcatatt
    27601 cggatcgaaa aaggaacggg tggctggggc ggcccgcttg agctgaaagc cacgccggga
    27661 aaaaaaatcg tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacag
    27721 cttactggct ggcaggctat tgacggattt agagaaggtg aacccgcgga ggcggaaatt
    27781 ggtgtcgcgg taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt
    27841 attcccacca ttaatatcca ctcgacgggc aagtccggtc cgctggcgca gtacattgtg
    27901 gaagatattt atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca
    27961 ccacaaccct cttccgtggg ccgtgactat gacaccagta agaaaatcac cgaacaaagc
    28021 gatggtttac tggcgaaggt gggaatgggc atggggtccg ccgttgcggt gctgtttcaa
    28081 tctggtcgtg acaccatcga cactgtatta aaaaccattc tgccgtttat ggcattcgtc
    28141 tcggcgctca ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt
    28201 gctccgctgg cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttccca
    28261 ctgctttcac ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt
    28321 ggcgtgcaga ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc
    28381 atcaacgcgc aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt
    28441 caggacacgg ttcgcgtcgg tgtcccttct gtactggtga gccgcttttt aaccggcgca
    28501 ccgactgtac tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat
    28561 gaccgttatt tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag
    28621 cgaccagatg ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt
    28681 cattcattgc cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg
    28741 gcagcatcgc tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact
    28801 gggtcatgtc accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca
    28861 tgtggcaggc cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt
    28921 taaggagtaa aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc
    28981 gttcctgtgc cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag
    29041 cgacaaagcc gcaaatgtgg cacaagaaat taacgccgaa tatggtgaaa gtatggcgta
    29101 cggttttggt gctgacgcca ctagcgagca aagcgttctg gcgctctctc gtggggtaga
    29161 tgaaatcttt ggtcgcgtgg atttgctggt ctatagcgcc ggaatagcaa aagcagcctt
    29221 tatcagcgac ttccagctcg gcgattttga ccgttcgcta caagtgaatc tggtgggtta
    29281 tttcctgtgt gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat
    29341 tattcagatc aactcgaaat ccggcaaagt gggcagcaaa cacaactctg gctacagcgc
    29401 agcgaaattt ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg
    29461 cattacggtg cattcactga tgctcggtaa cctgctgaaa tcgccgatgt tccagtcact
    29521 gttgccacaa tacgcgacca agctgggtat caaaccggat caagtcgagc agtattacat
    29581 cgacaaagta ccgctcaaac gcggctgcga ttatcaagat gtgctgaata tgctgctgtt
    29641 ctacgccagt cctaaggcgt cgtactgcac cggacagtcg atcaatgtca ccggcggtca
    29701 ggtgatgttc tgatcaacag cggagatcca ttaaggatct ccgtgagact atagaatgcc
    29761 tgatgcgcta cgctcatcag gcatacagga cttccgccac tacattaagg aaaagttatg
    29821 gtatccgcac tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc cttaggcggc
    29881 tggcaaattt ctcgttttaa ccgtgccttc gacacactat gccagcaagg gcgggttggc
    29941 gtgggccgtt ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgc gctggacgat
    30001 cagcagcgca tcgtcgacac cttgtttatg aaaggactga ccgtcttcgc ccgaccgcaa
    30061 aaaattcccg caattaccgg tatgcatgcg ggtgatttac agcccgatgt gatctttccc
    30121 catgatccac tatcacagaa tgctctatca ttggcgctta aactgaaacg tggataattt
    30181 cgttgtgaat gttacttgct tgcgaagtta tcattttgaa acctaaatca ggtaatcacg
    30241 cccatgaaac ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa gcagggtaaa
    30301 tgctcggttg aagaattggc gcaatacttt gacaccacag gcacaaccat tcgcaaagat
    30361 ctggtcattc tggaacatgc cggaaccgtc attcgtactt atggcggagt ggtgttgaat
    30421 aaagaggaat ccgatccgcc tatcgatcat aaaacactca tcaacaccca caagaaagag
    30481 ctgattgcag aagctgccgt tagttttatc catgatggcg attcgatcat tcttgatgct
    30541 ggcagtaccg ttttgcagat ggttcccctg ctctcgcgct ttaataacat cacggtgatg
    30601 accaacagcc tgcatatcgt caatgcgcta tccgaactgg ataacgaaca aactatcctg
    30661 atgccaggcg gaacgtttcg taaaaaatcg gcctcatttc acggacagct ggcagagaat
    30721 gccttcgaac atttcagttt cgataaattg tttatgggca ccgacggcat cgatctcaat
    30781 gcgggcgtaa ccaccttcaa cgaggtttat accgtcagta aggcaatgtg caatgccgcg
    30841 cgcgaagtga ttttgatggc ggactcatca aagtttggcc gtaaaagccc caacgtagtt
    30901 tgcagtcttg aaagcgtcga taagctgatt accgacgcag gtatcgatcc ggcgtttcgt
    30961 caggcgctgg aagagaaagg gatcgatgtg atcataaccg gagagagcaa tgagtgaagc
    31021 actactgaac gcgggacgtc agacgttaat gctggagttg caggaagcaa gccgtttacc
    31081 ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa
    31141 agtggtggtt tcgggaattg gcaaatcggg ccacattggt aagaaaatcg ccgcaacact
    31201 tgccagtacc ggcacaccgg ctttttttgt ccatccggca gaagcactgc acggcgatct
    31261 ggggatgatt gaaagccgcg atgtgatgct gtttatctct tactccggtg gcgcgaagga
    31321 actggatctg attattccgc gtctggaaga taaatctatc gcgctgctgg cgatgaccgg
    31381 caaaccgacg tcaccgctgg gcctggcggc aaaagcggtg ctggatatct ccgtagaacg
    31441 cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc tgatgatggg
    31501 tgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt aatgaagaag attttgcccg
    31561 ctcccaccca gccggggcac tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg
    31621 ccgtgacgat gccatcccac aggtggcgtt aaccgccagc gtgatggatg cgatgctgga
    31681 actcagccgc accggtctgg ggctggtggc ggtatgtgac gctcaacaac aggtacaagg
    31741 cgtctttacc gacggcgatt tacgtcgctg gctggttggc ggcggcgcac tcaccacgcc
    31801 agttaatgaa gcgatgacga caggcggcac cacattacag gcacaaagtc gcgccatcga
    31861 cgccaaagag atcctgatga agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg
    31921 caaactcacc ggcgcaatta acctgcagga tttctatcag gccgggatta tttaatcctt
    31981 caatcccaga cgtttcgcca gccgatgcag gttggcgacg tcggtttcca gcatccgcgc
    32041 gcaggcagcc cagttgtgat gattttgtgc cagtgcctgg cgaatggttt cacgctggaa
    32101 cgcttctgtc gcttcacgca ggttttgctt aacaacgggc accgccgccg cttctggcgg
    32161 cggcaacgtc acctcaggaa aagcaaaatg ttgtgcctca agaatcactt catcgccgct
    32221 gcgagtggct ctcgccagca ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt
    32281 ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg gcacttaata ccacgcggga
    32341 gagccccagc cgcaaacgac actgctcgca gaaatacccc gccagcagaa tgacatcatc
    32401 gccccgctca cgcagcggcg gcaccgaaag tggaaacacg ctcaggcgat gaaacaaatc
    32461 ggcgcggaat cgccctgcca gcacctcttc gcgtaagtcg cggttagtcg ccgccagcac
    32521 gcgcacatcg acccgcaaac tacggtcatc gccaacgcgc tgaatatcgc catactgcaa
    32581 caccctcagc agcttggcct gcaatgccaa cgacaactcg ccgatctcat cgagaaacag
    32641 agtgccgtta tccgccattt caaacttccc gctgcggtta ctgatagcgc cagtaaacgc
    32701 tcctttcaca tgcccgaaca actcactttc cgccacactt tccggcagtg cggcacagtt
    32761 gagatagacc agcggattca ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag
    32821 ctccttaccg gttcccgtct caccgctgat cagaacgttg agatcggacg ccgccacaat
    32881 ctcaatctct tttttcaatt gcgtcatgcc tggcgacaag ccaatcatct gcgtctgttt
    32941 caccgcttca aacggcgcgg catcgcccgg cagcatattc tggctttcca gttgttcaat
    33001 cagcaacgca ttgcttaacg ctcccgccgc cagcgcggca atcagccgta actcttcgtc
    33061 gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt
    33121 ttgcccggca aacaatggca gaccaacgca ggcgtgaacc ttcagactct cctgcccagg
    33181 aatcaaaccg tcatagggat cgggcaattc gctgtctgca ggaaagcgca ccacgtcccc
    33241 ggcgcgggca atcgcttcaa gccgtggatg cccttccagc gcaaagcgtc taccgagtac
    33301 atcctttgcc agaccgtcga tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa
    33361 cgcagacgca tcgcactcca gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc
    33421 ctgatgacca atcccacgct gcaattcgat ggcgatattc gccagcacat caacggaaaa
    33481 actcatcttt gcctcactgt caatttgact atagatattg tcatatcgac catttgattg
    33541 atagtcattt tgactactca ttaatgagca taattttatt tatagagtaa aaacaatcag
    33601 ataaaaaact ggcacgcaat ctgcaattag caagacatct ttttagaaca cgctgaataa
    33661 attgaggttg ctatgtctat tgtggtgaaa aataacattc attgggttgg tcaacgtgac
    33721 tgggaagtgc gtgattttca cggcacggaa tataaaacgc tgcgcggcag cagttacaac
    33781 agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc
    33841 cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg
    33901 attaaccatg cagaagagga ccacgctggg gcgctgaccg aactgatggc gcaaattccc
    33961 gatacgccga tctactgtac tgccaacgct atcgactcga taaatggtca tcaccatcat
    34021 ccggagtgga attttaatgt ggtgaaaact ggcgacacgc tggatatcgg caacggcaaa
    34081 cagctcattt ttgtcgaaac accaatgctg cactggccgg acagcatgat gacttacctg
    34141 acaggcgacg cggtgctgtt cagtaacgat gctttcggtc agcactactg cgacgagcat
    34201 ctgttcaacg atgaagtgga tcagacggag cttttcgagc agtgccagcg ttactacgcc
    34261 aatatcctga cgccgttcag ccgcctggta acgccgaaaa ttaccgagat cctgggcttt
    34321 aacttaccag tcgatatgat agcgacctcc cacggcgtgg tctggcgcga taacccgacg
    34381 caaattgtcg agctgtacct gaagtgggcc gctgattatc aggaagacag aatcaccatt
    34441 ttctacgaca ccatgtcgaa taacacccgc atgatggctg acgctatcgc ccagggaatt
    34501 gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac
    34561 gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat
    34621 aacgtgatga tgccgaaaat cgccgggctg gtggaggaga tgactggttt acgcttccgt
    34681 aacaaacgcg ccagtgcttt cggctctcac ggctggagcg gcggtgcggt ggatcgtctt
    34741 tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta gcctgaaagc caaatggcga
    34801 ccagaccagg acgctctgga gttatgtcgt gaacacggtc gcgaaatcgc ccgtcagtgg
    34861 gcgctcgcgc cgctgccgca gagcacggtg aatacggtgg ttaaagaaga aacctctgcc
    34921 accacgacgg ctgacctcgg cccacggatg cagtgcagcg tctgccagtg gatttacgat
    34981 ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg
    35041 gataacttcc tctgcccgga atgctccctc ggcaaagacg tctttgaaga actggcatcg
    35101 gaggcaaaat gagtaacggc attgtgatca ttggttcggg cttcgccgcc cgccaactgg
    35161 tgaaaaatat tcgcaaacag gacgccacta ttccattaac cctgattgcc gccgacagca
    35221 tggatgagta caacaaacct gacctcagcc atgttatcag tcaggggcaa cgtgccgacg
    35281 accttacccg ccagacggcg ggtgaatttg ccgagcagtt taatctgcgc ctgtttccgc
    35341 acacctgggt gacggatatc gatgccgaag cccgtgtggt gaaaagccag aataatcagt
    35401 ggcaatacga caagctagtg ctggcaaccg gtgccagcgc ctttgtcccg ccagtgcctg
    35461 ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac
    35521 tgcgggatgc ccgacgcgtg ttgattgtcg gtggtggctt gattggtagc gaactggcga
    35581 tggatttttg tcgtgcaggc aaagcggtca cgctaatcga caacgctgcc agtattctgg
    35641 cgtcattaat gccaccggaa gtaagcagcc gcttgcagca tcggttgacg gagatgggcg
    35701 ttcatctgtt gttgaaatct cagttgcagg ggctggaaaa aacagattct ggcattctgg
    35761 caacgctgga ccgccagcgc agtatcgaag tggatgcggt aattgccgcc accggactgc
    35821 gcccggaaac cgccctggca cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg
    35881 atagttatct gcaaaccagt aatgccgata tttacgcgct gggcgattgc gcggaaatta
    35941 acggtcaggt attgccgttc ctccagccga ttcaacttag cgcgatggtg ctggcaaaaa
    36001 atcttctcgg caataacacg ccactgaaac taccggcgat gctggtgaaa atcaaaacgc
    36061 cggaattacc gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc tggcagatta
    36121 ataccgaacg ccagggaatg gtggcgcgcg gcgttgacga tgctgaccag cttcgcgcct
    36181 ttgtggtcag tgaggatcgg atgaaagagg catttggatt gttgaaaacg ttgccgatgt
    36241 aggtgggcta ctgtgcctaa aatgtcggat gcgacgctgg cgcgtcttat ccaacctacg
    36301 gggagcgcat gtgttggtca catactgcgt tcatgtcgca tccacctact gtgcccaaaa
    36361 tgtcggatgc gacgctggcg cgtcttatcc gacctacggg gacgcatgtg taggccggat
    36421 aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca acacgtttta tccgttctgg
    36481 acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga caaaccgcca
    36541 tcacccgccg gtaaactctg tggaaagagc aatgtgaaat cagcgagata atgcgccaga
    36601 cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt agtgatacca
    36661 cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac
    36721 gcccacgcgc gttgattaac cggtgcctgc cagttcagcc actgctgcca gaaagtggcg
    36781 agatccagtt gattgtccac cagcggcatt gtcaccggat gcgtcactcc gtggcacgaa
    36841 gctgcgagtg cctccagagc acaagccgct tcaccttcat aacttaacgt ggctggcgca
    36901 cagcccagtg ccgccgccac ggcatcgaac aaccgcccac acgatgacgc cagcggcgcg
    36961 ttaattccac gctcaatggc ccgcgccagc acgctccagt tttgctgttg cacacttgct
    37021 gtttcagaat aattctgcca ctccggcaca aagcgcaggc actgcgccag caggtttcgc
    37081 cacggctgct tcgccgccaa atcgccaccc ggaagcgcca ctgcaggcaa gccgcccagg
    37141 tgctcacatt cgcgatagtt cacccgcaga cactcgccgc cccacaaagc gccgttctcc
    37201 cccataccga taccatcgag cgtcaaagca atgacatccc cgccatccag cggccactga
    37261 tgctctgcca gacacgccgc tgcgtgggca tgatgatgca gcaccgtttg cgtcggcata
    37321 ttcatttcgc tcgcccactg gctggagaca tagcccggat gcacgtcatg cacaacgtat
    37381 tgcggagtga aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca ctgcatctgg
    37441 atgccatcgt cacttaaatc gcccagatgc tgactcaaca ccgcttgttc accgcgcacc
    37501 aggcagaagg tgtttttcag atccgcgccg agacacagca caggcggaac atttttaaag
    37561 cccggaggca aagccagcgc atccggcaca tacccccgcg aacggcgcag catttcgccg
    37621 ctttcgcgca ccaccgaatc atccatccgc tgcacgatgt cgcggttatg tatcaagaat
    37681 ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt tgctgatagc tggtggttta
    37741 ccgctcaggt tgccggaggt catcaccagc gggcattgca gttcctgtaa cagcaaatgc
    37801 tggagcgggt tcgcaggcaa cattaccccg acttcgttaa ggtcaggggc gatatcatca
    37861 caaagctcag gaacgtattt tttatccacc agcacaatcg gcgcggcggg cgtggtaagc
    37921 aactggcgcg cagcgtctgg taaaccgtca gccactggca acatgactgc cagcggtttc
    37981 gccgggcggt gtttgcgctc ccgcagtgtc gccaccgcgt tactgttacg tgcatcgcag
    38041 gcaagatgaa atccgccaat ccctttgatg gcgacaattt tgcccatttt taactgtgcg
    38101 atagctgcct gtaatgccgc ctcttgttcc gcatgttcac catgacttac ccattcaaga
    38161 tgcgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc gagcgggtcg
    38221 cggtactctt tatcacaggc tggacacagc ggaaactccg ccattacggt aaacgggcgg
    38281 tcgtaaggca tggcgcgaat aatggtgaaa cgcgggccgc agtgggtaca gttgataaac
    38341 ggataacgat aacgccgttc gcctggggta ttcatttcgg caaggcaagc agggcaagta
    38401 gcggcatccg ggacaatttg cgtattcatg gcacctcccg cgctctggcg gatagtgaac
    38461 tcggtgggca gttgtgacca gataaacggc tcacgctcga cgctatcaat acgcgccagt
    38521 ggcggacagt gctgatgcaa ttgaacaaga aacgtttccg ggtcttccag cagccggact
    38581 tctacgccat cgccgtcatt acagacatcg ccgtgaagat ttaattgctg tgccagctgc
    38641 cagacaaacg gacgaaaacc gacgccctgc actttgccac gaatacgcag ttggacaccg
    38701 caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta acccgtccag
    38761 ccggacacga cagcgtcgca tccggcagtc acaggtcggc gataccgccc gcttcgtatt
    38821 ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat ttttagaaca
    38881 tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc aagtttatta cgatcgacac
    38941 aaattagcgc atgggtcggg caagccgcca tacacgccgg gccgtcttca cgatggttgc
    39001 acaggtcgca tttattggct tcagctttgt cagcccgtac attcagcccc acgccgctgt
    39061 tgcgaatcac cggacgtacc accacttcca tcgcgccata cgggcaagcc accacgcagg
    39121 ttttgcaacc aatacaacgt tcctgcatca catgaacaaa ccctttatca cggctgatag
    39181 caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa actgtcgccg
    39241 tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaatgtttcc ggggtcagcg
    39301 atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc cacttcacag gtacggcaac
    39361 caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc agcaatgaca
    39421 gttaatgcgc cgatacattc acaaatcatg ccagttttta atttactgtt atttaaggaa
    39481 attaatttct gtaatgcagg aaaaacgatg tcatcgacac tagtgacgat gacatgtgat
    39541 gacaatgttt atcgcgaaag ggcaatgagt gagtcgcggc agatcaattt tccgctgaag
    39601 gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc aataatttcc
    39661 tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacgat atagggggca
    39721 attgcgatat catcgaatcc gataactgac acctgctctg gcaccgctac gccgcgctcg
    39781 tgcaacgctt tcatcgcacc tatcgccata tcgtcgttac tggcaactaa cgcgctaaat
    39841 ttagccccac gttcgagcaa catttctacc ccttcggccc cgctggcagg cgtccatcta
    39901 ccgttagcga taagtttttc attgagcgca ataccgtgct gcgccagcgc gtctttatat
    39961 ccggcaagac gttcaacgct ggtgggagaa tccatcgaac cggtaaggaa agcaatctcc
    40021 tgatgtccgg cgtttatcaa ctctgccacg gcgttaaaac tggtctgttt atgatcgcac
    40081 cagacgctat ggctgctgtt tttgcgcagg cggcgattaa gcaccattat cggctgactg
    40141 tgcgcgtcaa tgatgtcatc gatctcatcc acgcttaaaa aacgcgggta aatcataatg
    40201 gcgtcgcagc gcaaatccag cagatactga atcgcctggc gctcttcttc ggcgctgtgt
    40261 ttaccatctg ccaatagcaa ctgccgccct ttctcttccg ccattcgcgc ggcatgaaac
    40321 agtaattcac taaaataaat gccgtggtaa agcgtgttgg tcactaccag ccccagcgtc
    40381 tgagtactct tcgccgacaa attgcgcgcc agcaagtttg ggcggtaacc gctctcttct
    40441 accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc tgaaagcacg
    40501 cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc
    40561 attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttgccg attgtccttg
    40621 cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca aatataaaaa atgaacaatt
    40681 aactctcttg cttatttagt gacaactatt catgattttg tgaaaccggt ttcctaattc
    40741 cgtttcagca tcggcatttt tccgttacgt cgactgataa caactacatc taccctactg
    40801 ataacaggat aaaatccgat ggccaaaaat tatgcggcgc tggcacgctc ggtgatagcg
    40861 gcactgggcg gcgttgataa catctcggcg gtcacgcact gtatgacgcg tttgcgcttt
    40921 gttatcaaag atgaccagct tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc
    40981 ggcgtggtac gtagtgacaa ccagtgtcag gtgattatcg gcaataccgt ttcacaagcc
    41041 tttcaggaag tcgtcagcct actgccggga gatatgcagc ccgcactgcc cgtgggtaaa
    41101 cccaaactca cgctgcgtcg cattggtgca gggatcctcg atgcgctgat cggcaccatg
    41161 tcaccgctga tcccggcgat tatcggcgga tcgatggtca aactgctggc gatgatcctc
    41221 gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt
    41281 gacggtgctt tcttcttcct gccgctgatg gtcgcggcat ctgccgccat caaatttaaa
    41341 accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa
    41401 ctgatggcga aagcggccca gggtgaacat gtcgaatttg ccctgattcc ggtcaccgcg
    41461 gtgaaataca cctacacagt gatcccggcg ctggtgatga cctggtgcct gtcatatatc
    41521 gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa acttcctcaa gccaatgctg
    41581 attgtgttga ttgccgcacc gctggcaatc ctgctgattg gcccgattgg tatctggatc
    41641 ggtagcgcca tttcggcgct ggtttacacc attcatggtt atctgggctg gctttcagtc
    41701 gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg
    41761 ccaaccatca ttcagaccat tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag
    41821 atcggcgcta acctgtcgct gggcggttca tcactggcgg tggcgtggaa aacgaaaaac
    41881 ccggaactgc gccagacggc gctggctgcg gcggcatcag ccattatggc ggggatttcc
    41941 gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc
    42001 agcggcttta tttgcggcgc ggttgccggt atggcggggc ttgccagcca ctcaatggca
    42061 gcgccggggc tatttaccag cgtgcagttc ttcgatccgg cgaatccaat gagcatcgtc
    42121 tgggtgttcg cggtcatggc gctggcggtc atgctgtcgt ttatcctcac actgttgctc
    42181 ggctttgagg atattcctgt tgaggaagcg actgccgagg cgcgaaagca tcagagcgta
    42241 caaccgaccg tcgccaaaga agtaagtctt aattgaggat gaaaatgtca gtatttccag
    42301 aaggtttttt atggggcggc gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag
    42361 gtggcaaagg gctgaccact gtcgatatga tcccacacgg cgagcatcga atggcggtga
    42421 aactggggct ggaaaaacgt tttcagttgc gcgatgacga gttttatccc agccatgagg
    42481 cgacggattt ttatcatcgt tataaagaag atatcgccct gatggcagag atgggattca
    42541 aggttttccg tacctcaatt gcctggagcc gtctctttcc gcagggcgat gaaatcacgc
    42601 ccaatcagca gggcattgct ttttatcgtt ctgtctttga agagtgtaaa aagtacggta
    42661 tcgaaccgct ggtcacgttg tgccacttcg atgtgccgat gcatctggtc accgaatatg
    42721 gctcctggcg taaccgcaag ctggtggagt ttttcagccg ctacgcccgg acctgctttg
    42781 aagcatttga tggtctggtg aaatactggc ttaccttcaa tgaaatcaac attatgttgc
    42841 atagcccgtt ctccggcgcg ggtctggtgt ttgaagaagg tgaaaatcag gatcaggtga
    42901 aatatcaggc cgcgcatcac cagctggttg ccagtgcgct agccaccaaa atcgcccatg
    42961 aggttaaccc gcaaaatcag gtgggctgta tgctggcggg cggtaacttc tacccttaca
    43021 gttgcaagcc ggaagatgtc tgggcggcgc tggagaaaga ccgggaaaac ctgtttttta
    43081 tcgatgtgca ggcgcggggc gcgtatccgg cttactctgc ccgcgtattc cgcgaaaaag
    43141 gggtaaccat cgacaaagca ccgggcgatg atgaaatcct gaaaaacacc gtcgattttg
    43201 tctctttcag ctattacgcc tcgcgctgcg cctcggcgga gatgaacgcc aacaatagca
    43261 gtgcggcgaa cgtagtgaaa tcgctgcgta acccgtatct gcaagtgagc gactggggct
    43321 ggggtattga tccactcggt ctgcgtatca ccatgaacat gatgtacgac cgttatcaga
    43381 agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga tgaacttgct gccaatggcg
    43441 agattaacga tgactatcgc atcagctatt tacgcgaaca tatccgcgca atgggcgaag
    43501 cgattgcaga tggcattccg ctgatgggct acaccacatg gggctgtatt gatttagttt
    43561 ccgcctctac gggtgaaatg agcaaacgct acggttttgt ctacgtagac cgtgacgacg
    43621 caggcaacgg cacgctgacg cgcacacgta agaaatcgtt ctggtggtat aaaaaagtga
    43681 ttgccagtaa tggtgaagat ttagagtaag caacagtgcc ggatgcggcg tgaacgcctt
    43741 atccggccta catgtcgcac gaaccaggaa ggcccgataa gcgcagcgca tcgggcaatc
    43801 tggggtttgg catcagtcaa aatcaaagaa accattccgg cttaacgcca aatcgggctg
    43861 aaagtgcctt aatgtgggat atcgtcaggg atcgttgtcc agacaaaatc tgactgacca
    43921 gggatttaga gccgatttcg ttcttcaaat ccgcataggt cagattatgt tgatcaatca
    43981 gcgtgcgaag taacgccacg ccaacaggca ttgccgcaac ggctttgtcg aactctgcaa
    44041 acttttcgtt gttgttttca tactctgcaa tgcggctcgc cagaaagtcg ataagtggat
    44101 tggtgtcatc ttcttcaatc aaatattcga ccagttccag tgcttcacga taatcatctt
    44161 ctgagtcact gcctcccaga aaggggactg cattgaccag ttctcttgtt gctttaacag
    44221 cgcgtgcagc attagcagtc attctttgtt ctctctgtag taacgtgtca gtttgtcata
    44281 ctctgcgtga ttcgttatgt gcttcacaaa gaaccgctta ttaacaaagt ttatgtaggc
    44341 aataacccta agattgttcc cgccgacatc cagaacgtac catttgttgc gatatctaaa
    44401 attgtcgagg ttgggaaacg ccgttcgcat ttcttctggt gtagaaaaat ccgtttcctt
    44461 aataacccga tacaatgcct gatgagccag agcgtcgtta ggatatttac gcgcagactc
    44521 ttcaaatggc gctttagaaa tgatgtgcat ttttaagttt taacttcctt atagtaagta
    44581 aacagccaca tctgcgatgt tctatcctta tgtgttcgga aactatagct catcaaatcc
    44641 aagttcacaa tatgtaaaca cttgtgcgcg atataattac ccccaaaatc ctcatagcca
    44701 ctctgcaaaa agtcagttct ggttcacccc tcaagcacat ccccctttat caagcaacgc
    44761 tgcgtctgcg ccagatcttc cgtttgcggg tcgcgcccgg cgatgggttt gaggacgagt
    44821 ttacggttgg gggtataagt aaaacgcgtg agcgcgggat agcttaactt gccaaaccag
    44881 ccgtgaacac gctgccattt ggcgtctttt acctgataca gactggttat ccagtaacca
    44941 gaattaaact gcatatccag caattgcgtg tgaccattgc cttgcaaatc cagcaaatca
    45001 tctacgccag ttttgcttgc ggtgtagaat cccctgggtt caaagacaca gggacggcct
    45061 tcgcgcgtga acgtcataag aattaaatgt gcataaggag ccagaccatt accggtactt
    45121 ggtaaccaga taaccagatc ctgaatgccg ttacgatcaa gatcggcgcg atagagttgc
    45181 cctttgcccg ctatgtttgc cagcataccc acaggcacag tccaggattt accggcgcgg
    45241 tctttagcgg ttaattgcca gtaatcttct tcatttggca tcttctcaag cgtcacgcca
    45301 gcaacgccca ctttcctaaa atgccaggtt tgctcggcat cataaggcat ggtcaggaga
    45361 ggtttcagcg cccgcttttg cgcgtattcg ctgtcactca ttggcagata ttcggtaaag
    45421 ctggtggcct gtgcaggcag cactaagctc aataaaaaag cgcacatccc tgttctgatg
    45481 ttcaattccc tgaattccct gatggagaat tattcttctt ccaccgctaa ctgcgcaaat
    45541 ccaccatttc cttcccagcc ttccagtcgt tgataaacgg tttctaccgc atctttaatc
    45601 ggctgggtca tcgggtagta aaagccgacg atatccggct gaatgccgag gaaaatcact
    45661 tcaccaatgt cttcttttaa ctggtcgata aggtaattca acggcatgtt atgggtagtc
    45721 atcataaaca tctcggcgat atcatccggg tcgatgatgc ggatttcgcc ggggtttagc
    45781 cccatatccg tggcgtcgac aatcagcagt cgtgtcgggc gcagttcacg gatggcgacg
    45841 atgtcgtttt ccggtgcact accgccgtca atcaccaccc agttaccttt cggcgcggcg
    45901 gcgcactttt ccgccagcag cgggccagcg ccatcatcgc ccatcatgct attgccaaca
    45961 cagagtaaaa cgtcagtcac gcagtctcct caccatcaaa tagatggcgt tttcctgatg
    46021 aatatcatgc agcatactta acagcattgt actccattcc ttctgctgcg aagtttgcgc
    46081 tgcccgggct ttatcgaatg cgttggccag catcggcaca tggttaatgt cgatgacgat
    46141 ctcgccatat ttcggtacgc cttccatttt gcggcgggct tcgctgcccg cctccagagt
    46201 ggcaatccat gccagatatt catcccatgg gcaggtgagc gccgcttcca ggcaatcgat
    46261 aaccccaagg tggtgaccaa tcgccaggct gtaatagacc acctgctgcg cctcggcggg
    46321 cgtggcatcg ttctcatcaa taaatttacg gctcagttga ctgaacacca ccttttcact
    46381 catcggatac gcgcctcttc aacaacatga ttcagatggc tgacaatctc gttcagacgc
    46441 ggatcgtttt ccgcttccag ccagcgtgca acctgttctt cgccctgccc taactgtgta
    46501 aggtaatcat cggcaatctg acgaccgtaa cgataacccg ccaggcgacg tgcttcgcga
    46561 tccactttca cgcgcagcgg ctgcaccata tcgccatgca gaatctcagc cggttgttca
    46621 tcctgctcac ccgggccacg ggcgtgaatt ttctgctcca gcaggccgag tgccattgca
    46681 aagccgtaca gcgtggcggc aggcgttggc gggcaacccg gaatgtagac atctaccggg
    46741 acgattttat ccgtaccgcc ccacacgcag tagagatcgt ggaaaatacc accgctgtta
    46801 ccgcaggcac cgtaggagat acaaattttc gggtccggcg cggactgcca cgcacgcagc
    46861 gcaggggatc gcattgcacg ggtgaccgcg ccggtaaaca gtaaaatatc cgcatgacgc
    46921 ggtgaaggaa cgactttaat gccgaagcgt tctgcatcaa acagcggcga taaagtggcg
    46981 aaaatttcga tttcgcagcc gttgcagccg ccgcagtcca cgcggtaaac ataggcagaa
    47041 cgtttgattt ttttcagtaa cgacgccttc atgctggcga tggattcatc caccgtcatc
    47101 gggaccggaa tgccgttggc gtcacggggg cctaataaat tgctcatcag atggcctctt
    47161 tcatatggcg agtcagttca atacggtcgg acggcaccag gcatttctgg cgcttacatt
    47221 ccgggcaagt ctcaaagctt tcgcggtggt tttccgcgcg gctgtcacca ttgtgcttaa
    47281 gcagcgcaat ggcgtagtcg atctctttct ggacggcgaa aggacgattg cagacgcgac
    47341 agctgcacag cacaaaacgg gactgttgca ggaaatcttc tttcttccac accgccagtt
    47401 cgtactcttg cgacagtttg atcgctgccg tcgggcagac ttcttcgcag cgtccacaga
    47461 agatgcagcg cccgagatta aactcccacg ccagctcacc ggtggcgagg tcagtttcaa
    47521 ccgttaaggc gtttgacggg caggcattga cgcaggccgc acagccgatg cactgctgcg
    47581 ggttctgctc cggcttacca cggaagtttt tatcaaccgc aatcggctcc agcggataag
    47641 acgaggtcgc cgtgccggtt ttgatgactt ttttgataaa ggtaaacatg gcgattcctt
    47701 atttcagcgg cgagttttta cgctcaatgc tgtaacgctc gagttctttg tacggcacca
    47761 ctttgctctt tttcttacgc acatcgacca cggtcatgcg gtcggtacag gagtagcaag
    47821 ggtccaggct accgataatc agcggcgcat cggaaacggt gttaccgcgc agcatgtagc
    47881 gcagggtcgg ccagttcgcg taggtcgcgg cacggcagcg ccagcggtac agcttctggt
    47941 tgtcgccggt catgctccag tggatatcgt cgccgcgcgg cgcttcggca aagcccagcg
    48001 cgaaacggtg cggaatgtag gtaaagcctt ccaccatcag cggaccgccc ggcaggttat
    48061 ccagaccgta gtcgatcatg ttcagcgcgg tataaacttc gttgatacgc actttcagac
    48121 gggaaataac gtcgcagccc tgctcgctgt ggacttccat tggcagcagg ccataaccga
    48181 caaacgggtg atcggcgcga gtatcgcggg cgtgaccgct ggcgcgaacc atcgggccaa
    48241 cgttactgaa atcgcgggcg atttccgggt ccagacgacc aatgccgacg gtgcgctgtt
    48301 ccatgttcgg cgtactcagc agcacatcca ccagctcctg cacttcacga cgcatctgtt
    48361 gcgccagctg gcgggtctgg atcatgtcat ctttcaacag atcgcgacga atcccgccga
    48421 tcaggttcag gccgtaggtt ttacgcgcac cggtgaggat ctctgccatt ttcatggagg
    48481 tttcacgcac gcggaagaac tgcataaagc cggagtcaaa gccggtaaag tggcaggcga
    48541 gaccgaggtt gagcagatgc gagtgcaggc gttccacctc cagcagaatg gcgcggatca
    48601 tctgcgcacg ttctggcacc tgaataccca tcgcgttttc taccgacgtg gtgtaggcgg
    48661 tgctgtgggc aaagccgcag atcccgcaca cacggtcaga gaggaaggtc acttcgttgt
    48721 aacccatacg ggtttccgcc agtttttcca tgccgcgatg aacgtagaac agacggtagt
    48781 cggcgtcgat aatgttttcg ccatcgacga acagacggaa gtggcccggt tcgtcagaag
    48841 tgacgtgcag cggaccaatc ggcacgacgt tgtttttctt gtcgcccagt tcgttgatga
    48901 actcgtaggt ttcagcatcg gtagtcggtg ccggacgctg acgataatcc atgctgtctt
    48961 tacgcagcgg ataaagttca tccggccagt catccggcag caccagacga cgttcatccg
    49021 gcagaccaac cggaatcaaa ccgtacatat cgcgcacttc gcgctcgccc cacaccgccg
    49081 ccggaacgcg cggcgtcacg gacggatact ccggtttgtt ggcgtcaact tcgacgcgaa
    49141 ccgttaccca acacttggtg cccttctcca tcgacagcac gtaataaacg gcgtaatgac
    49201 cattcagttt gcgttcgtcg ttaccaaaca gcaccgacag ccagccaccc tgcttgtagt
    49261 agagaaactc caccacttcc ggcaggtagt tcaccttcac ggtcacggtc agctgatctt
    49321 tggtctgcca ggcgtggtcc agcacgacgc ccggaaatgc ctcattcagc gcggcgagat
    49381 aatgttgacc taatttttct tcagacatgc tcaaactctc tttaatcacg ccgccagcaa
    49441 ggagacgaac gctaaaaatg caaagccaaa cccagcccag gtaatgcgcg gagtaatatc
    49501 aagacgcaga cgggccatgc tgttttcgaa cagcgcgata accaggacgc cgacgaccag
    49561 tttgacgatg gcaatcacca gcgccagcag cagcccaccg gcggtgaagg tttccatctg
    49621 tccccacggg ataaatacgc cgacgaacat ctgcagcacc accagctgtt tcaggctgat
    49681 accccatttc atgacgccaa agccgctgcc gctgtattca gagagcgggc cttcctgcag
    49741 ctcctgctcg gcttccgcca ggtcgaacgg cagtttgccc atttcgataa aggtggcgaa
    49801 cgcacaggca caaagcgcca gtaccagcgg gatgctctgg ctcagcggcc agtgataaac
    49861 ggtgtcggtg atgttgctga tgttggtgga accggcaacc tgtgcggcaa cccacagacc
    49921 aagcagcagc atcggttcga ccagcacgcc aagcatcgct tcacggctcg cgccgatagc
    49981 ggtaaacggg ctaccggtat ccagaccaga aatggcaaag aagaaacgcg cgatggcaaa
    50041 gagatacagt aaggtgatta aatcacccag ttgcggcagc ggagaaccga cggtcaccac
    50101 cggcagcgca gtagcgatag tcagcatgac gcccaccatc acatacggcg tcaggcggaa
    50161 cacccagccg gaggcatccg ggccaacgct ctggcggccc agcagtttga taatgtcacg
    50221 atactcctgc aacacacccg gcccacgacg gttatgtaag cgggctcgcg ccacgcgggt
    50281 aataccggag agcagcggcg caacggcaaa tagcaccagc gcctgaatta acggatataa
    50341 aacactcatt ctcaggctcc tcgtgaaaca ataatcacca ccagtaccgc cagttcaacc
    50401 agcgccatcc ggcggaacag caacgcactc ccctcgcact gccagcccgg caccagagac
    50461 accggattca gccatttgcg tagtttcagc accggcgcaa acgcctgttt caccggcatg
    50521 gcaaaaccgt gagcggtaat caccattgat ttttcgtggt cgtagccgca cacccaggcc
    50581 gcgccgcggg aacgcgatgg caaacgatcg cctttgcaaa tcgccataat gatgaatggc
    50641 agcagcggac aggcaatcag cagcaatgtg atcattggtt gagaaacggt ggtgttagca
    50701 ggctccagcg gcagaggtac agcagcagac agcatcggca gcagccacgg cgcagcaaca
    50761 ccgccaatta cgcagcaaat cgccagtgcc actacgctta ccctcatcag gagcggcgca
    50821 caggtggcgt tttcggcttc tttggtacgc ggcgcgccga ggaacgtgac gccatagact
    50881 ttcgccatac acatcaccgc cagcgcaccg gtaattgcca gacccacagc gagcagcggc
    50941 cccagcagac gggcaacaaa cgcgccgcta ttgctcagtt tgaagaagga ttgatagata
    51001 acccattccc cggcaaaccc gttcagcggc ggcagcgcag ccattgccat taacccgact
    51061 aacatggcga tggagataac cggcattttc ttgccaatac caccgagttt ttcgatatcg
    51121 cgatgaccgg tacggaacca gacgctcccc gccccgagga acagtacgct tttgaacagg
    51181 ctatggttaa gcagatggta cagaccaccg accaggccaa gagcaatcag cgccggttgt
    51241 tcgagcgcga tacccgttac gccagcgccc agccccagca ggataatgcc gatattttcc
    51301 agggtgtggt aagccagcag gcgctggatg ttgtgctcca tcagcgcata cagaccaccg
    51361 acaaacgcgg tgatcatgcc gagcaccagc agcgcgatcc cccaccacag cggtgcatta
    51421 ccgcccagca gtgacagggt taaaatgccc agcaggccaa ttttcatgac taccgtagaa
    51481 aacaacgcgg cagctggcgc agaggcgtta gcatgtgcct gcggcaccca gccgtgcagc
    51541 ggaataatcc cggcgagcag gccaaagcca atcacaccga gcagccagat atcggaaccg
    51601 agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc ataacgctgc
    51661 cacagcagcc agcaagcaat cgccagcagc agagtgccaa gacgccccag cgcaaaccac
    51721 agtttgccct ctttgctgtt gctagtgagg aacaccgcgc acagggccat gatttcggcc
    51781 attaccacga acatgccgag gttgctggca atgacggcgc agacggcggc agccatcaac
    51841 atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc aatgttatag
    51901 aggctgacaa acagaccgca aaggccgagg gtaatcagcc aaatcgcgtt aagcggggag
    51961 atttgcacat cgtagcttac cagcgacagc gcaccgctca cgccaaccgc gccagtcagc
    52021 acagtgaagc ccgcggctgc cgtatacagg ctaccaaccg cgccgccaat tccggctatc
    52081 cagccactta gcgtcttttg aaaagaaaag agaaatgcca gaacagcggc ggcgacaaac
    52141 cacgccacgc cgctattgat cagggaaatt gcgctcattt agcctctcca ctttgagcct
    52201 gctgaaacaa ggtgagatcg ccaaagtccg tgttaaaggt cagctcacgc ttacgtttgc
    52261 tgacgcgggc gatatcggtg ttatccacca gatgcagggc tttagtcggg cacatccgca
    52321 cgcaggccgg accttgttca tcaaagctac aaaggtcaca tttgacggcg atcgcgcgaa
    52381 tacctggcac ccagtcaagc aatgtgctga cacgcgccgg agcaggcggt gccggtggcg
    52441 ctttcggggt attggcgttt gccggaatat ccagcggacg gctgccggaa aattcaattg
    52501 cgccaaacgg gcaggcgatg ccgcacagct tgcagcttac gcacaggctt tcattcaact
    52561 gcacggcccc atcaacgcgg gtgatggcgt taaccgggca gaccaccgcg cagggtgcat
    52621 cttcacagtg gtgacagagc tgcggcgcag attctttttc attcagcatc actctcaggc
    52681 gcggcattga ttgcaggccg tgctggcgat gcgtctctga acaggcggcc tcacaagtgt
    52741 ggcagccgat acagagcgtg gagtcagcaa ttacaaaacg attcaccagg cattcctcag
    52801 gtgattgtca tttttgacga aaacatgccg atgaaatgtc attttcgaca ctcatcgaca
    52861 cgcccatccc caaacaggcg taacgcctgc aaaacgggca aagcctcagc tcatgctgcc
    52921 gggctttgtc cctttaccag ttggcttaaa ttcaccggca cattgttttc aatagcggtg
    52981 tataagctgt cgtaaacgcc agcgattttt tcgagatagt gttccagaac ctgttcgcga
    53041 tcgcggatgt tgaccttgcc atcaatcatg ccgtcaatgc tcagttgcgc ctgcgcaatc
    53101 cgctgtttgt cttcgccatc ttttgtttcg acgtaatact cgatggtgtg gctgttaaag
    53161 ccatccgcca gggtgacgta aatgcgaaaa tgttcaaaaa ggacgaaaca gagttctttg
    53221 tccggcgcgc agctcatggc gtgatgcagg cgcgctttcg ataacgtgat cccctgaacc
    53281 agcgaattgc agtagatgtg ccactggtcc tgtaggcgac gatgccgctg tgcgatgtaa
    53341 tcggctttct cgcttatttc ccaaatagtc attgtcaggt tacccgttta acagagatgc
    53401 cagctttaag cattttctgt gccaactttt aatttattgt tattaaagag atttttaagc
    53461 taaagatgaa tttcgtcgcc gtgtcgacgt gtcatttcga catcatcgac attattcacc
    53521 gcagggataa tcaacactgg cacaattatt gcttgtagct ggcaatagtt aatgggaggc
    53581 gatatgcacg aaataaccct ctgccaacgg gcactggaat tgatcgaaca gcaggccgca
    53641 aaacacggcg caaaacgcgt aactggggtc tggctcaaaa ttggcgcatt ttcttgtgtc
    53701 gaaaccagct ctcttgcctt ttgttttgat ctggtttgcc gcggcagcgt ggcggaaggt
    53761 tgtaaactgc acctcgaaga acaagaggcc gaatgctggt gtgaaacatg ccaacagtat
    53821 gtgacgctac tgacccagcg cgtccgccgc tgtccacagt gtcatggtga catgctgcag
    53881 attgtggcag acgacggttt acagattcgg cggatagaaa tagaccagga gtgagcgatg
    53941 tgtacaacat gcggttgcgg tgaaggcaac ctgtatatcg agggtgatga acataaccct
    54001 cattccgcgt ttcgtagcgc gccatttgcc ccggcggcac gcccgaagat gaaaatcacc
    54061 ggcattaaag cgcctgaatt tacccccagc cagactgaag aaggcgacct gcattacggt
    54121 catggcgaag cgggcactca cgcaccgggc atgagccagc gtcggatgct ggaagtcgaa
    54181 attgacgtgc tggacaaaaa taaccgtctg gctgaacgca accgcgcgcg ctttgctgcc
    54241 cgcaagcaac tggtgctcaa cctggtttcc agcccaggtt ccggtaaaac caccctgctg
    54301 acggaaacct taatgcgcct gaaagacagc gttccgtgcg cggttattga aggtgaccag
    54361 caaaccgtga acgatgccgc acgcattcgc gctaccggca caccagcgat tcaggtgaac
    54421 accggtaaag gctgccatct tgacgcacag atgattgccg acgccgcacc gcgtctgcca
    54481 ctggacgata acggtattct gtttatcgaa aacgttggca acctcgtatg cccggccagc
    54541 ttcgatctcg gtgaaaaaca caaagtggcg gtgctttccg ttaccgaagg tgaagacaaa
    54601 ccgctgaaat atccgcatat gtttgccgcc gcctcgctga tgctgctcaa caaagttgac
    54661 ctgttgccgt atctcaactt tgacgttgag aagtgcctcg cctgcgcccg cgaagtcaat
    54721 ccagaaattg aaatcatcct tatttccgcc accagcggtg aagggatgga ccagtggctg
    54781 aactggctgg agacacagcg atgtgcatag gcgttcccgg ccagatccgc accattgacg
    54841 gcaaccaggc aaaagtcgac gtctgcggca ttcagcgcga tgtcgattta acgttagtcg
    54901 gcagctgcga tgaaaacggt cagccgcgcg tgggccagtg ggtactggta cacgttggct
    54961 ttgccatgag cgtaattaat gaagccgaag cacgcgacac tctcgacgcc ttacaaaaca
    55021 tgtttgacgt tgagccggat gtcggcgcgc tgttgtatgg cgaggaaaaa taatgcgttt
    55081 tgttgatgaa tatcgcgcgc cggaacaggt gatgcagtta attgagcatc tgcgcgaacg
    55141 tgcttcacat ctctcttaca ccgccgaacg ccctctgcgg attatggaag tgtgcggtgg
    55201 tcatacccac gctattttta aattcggcct cgaccagtta ctgcctgaaa acgttgagtt
    55261 tatccacggt ccgggttgcc cggtgtgcgt actgccgatg ggtagaatcg acacctgcgt
    55321 ggagattgcc agccatccgg aagtcatctt ctgtaccttt ggcgacgcca tgcgcgtacc
    55381 agggaaacag ggatcgctgt tgcaggcaaa agcacgcggt gccgatgtgc gcatcgttta
    55441 ctcgccgatg gatgcgttga aactggcgca ggagaatcca acccgcaaag tggtgttctt
    55501 cggcttaggt tttgaaacca ccatgccgac caccgccatc actctgcaac aggcgaaagc
    55561 gcgtgatgtg cagaattttt acttcttctg ccagcacatt acgcttatcc cgacgctgcg
    55621 cagtttgctg gaacagccgg ataacggtat cgacgcgttc ctcgcgccgg gccacgttag
    55681 tatggttatc ggcactgatg cctataattt tatcgccagc gattttcatc gtccgctggt
    55741 ggtcgctggt ttcgaacccc ttgatctact gcaaggcgtg gtcatgctgg tggagcagaa
    55801 aatagcggcc cacagcaagg tagagaatca gtatcgtcgg gtggtaccgg atgccggtaa
    55861 cctgctggcg caacaggcga ttgccgatgt gttctgtgtc aacggcgaca gcgaatggcg
    55921 cggcttaggc gtgattgaat cttctggcgt gcacctgacg ccggattatc aacgattcga
    55981 tgccgaagca catttccgcc cggcaccgca gcaggtctgc gatgacccgc gcgcgcgttg
    56041 tggtgaggta ttaacgggca aatgtaagcc gcatcaatgc ccgctgtttg gtaacacctg
    56101 taatcctcaa accgcatttg gtgcactgat ggtctcctcc gaaggagcgt gcgccgcgtg
    56161 gtatcagtat cgtcagcagg agagtgaagc gtgaataata tccaactcgc ccacggcagc
    56221 ggcggccagg cgatgcagca attaatcaac agcctgttta tggaagcctt tgccaacccg
    56281 tggctggcag agcaggaaga tcaggcacgt cttgatctgg cgcagctggt agcagaaggc
    56341 gatcgtctgg cgttctccac cgacagttac gtcattgacc cgctgttctt tcctggcggt
    56401 aatatcggca agctggcgat ttgcggcact gcgaatgacg ttgcggtcag tggcgctatt
    56461 ccgcgctatc tctcctgtgg ctttatcctc gaagaaggat tgccgatgga gacactgaaa
    56521 gccgtagtga ccagcatggc agaaaccgcc cgcacggcag gcattgccat cgttactggc
    56581 gatactaaag tggtgcagcg cggcgcggca gataaactgt ttatcaacac cgctggcatg
    56641 ggcgcaattc cggcgaatat tcactggggc gcacagacgc taaccgcagg cgatgtatta
    56701 ctggttagcg gcacgctcgg cgaccacggg gcgactatcc ttaacctgcg tgagcagctg
    56761 gggctggatg gcgaactggt cagcgactgc gcggtactga cgccgcttat tcagacgctg
    56821 cgtgacattc ccggcgtgaa agcgctgcgt gatgccaccc gtggtggtgt gaacgcggta
    56881 gttcatgagt tcgcggcagc ctgcggttgt ggtattgaaa tttcagaagc ggcactgccg
    56941 gttaaacctg ccgtgcgcgg cgtttgcgaa ctgctgggac tggacgccct taactttgcc
    57001 aacgaaggca aactggtgat cgccgttgaa cgcaacgcgg cagagcaagt gctggcagcg
    57061 ttacattccc atccactggg aaaagacgcg gcgctgattg gtgaagtggt ggaacgtaaa
    57121 ggtgttcgtc ttgccggtct gtatggcgtg aaacgaaccc tcgatttacc acacgccgaa
    57181 ccgcttccgc gtatatgcta ataaaattct aaatctccta tagttagtca atgacctttt
    57241 gcaccgcttt gcggtgcttt cctggaagaa caaaatgtca tatacaccga tgagtgatct
    57301 cggacagcaa gggttgttcg acatcactcg gacactattg cagcagcccg atctggcctc
    57361 gctgtgtgag gctctttcgc aactggtaaa gcgttctgcg ctcgccgaca acgcggctat
    57421 tgtgttgtgg caagcgcaga ctcaacgtgc gtcttattac gcatcgcgtg aaaaagacac
    57481 ccccattaaa tatgaagacg aaactgttct ggcacacggt ccggtacgcc gcattttgtc
    57541 gcgccctgat acgctgcatt gcagttacga agaattttgt gaaacctggc cgcagctggg
    57601 ctatacccca aatttggtca ctattgcctg atgccactgg cggcggaagg gcatattttt
    57661 ggtggctgtg aatttattcg ttatgacgat cgtccctgga gcgaaaaaga gttcaatcgt
    57721 ctgcaaacat ttacgcagat cgtttctgtc gtcaccgaac aaatccagag ccgcgtcgtc
    57781 aacaatgtcg actatgagtt gttatgccgg gagcgcgata acttccgcat cctggttgcc
    57841 attaccaacg cggtgctttc ccgcctggat atggacgaac tggtcagcga agtcgccaaa
    57901 gaaatccatt actatttcga cattgacgat atcagcattg tcttacgcag ccaccgtaaa
    57961 aacaaactca acatctactc cactcactat cttgataaac aacatcccgc ccacgaacag
    58021 agcgaagtcg atgaagccgg aaccctcacc gaacgcgtgt tcaaaagtaa agagatgctg
    58081 ctgatcaatc tccacgagcg ggacgattta gccccctatg aacgcatgtt gttcgacacc
    58141 tggggcaacc agattcaaac cttgtgcctg ttaccgctga tgtctggcga caccatgctg
    58201 ggcgtgctga aactggcgca atgcgaagag aaagtgttta ccactaccaa tctgaatttg
    58261 ctgcgccaga ttgccgaacg tgtggcaatc gctgtcgata acgccctcgc ctatcaggaa
    58321 atccatcgtc tgaaagaacg gctggttgat gaaaacctcg ccctgaccga gcagctcaac
    58381 aatgttgata gtgaatttgg cgagattatt ggccgcagcg aagccatgta cagcgtgctt
    58441 aaacaagttg aaatggtggc gcaaagtgac agtaccgtgc tgatcctcgg tgaaactggc
    58501 acgggtaaag agctgattgc ccgcgcgatc cataatctca gtgggcgtaa taatcgccgc
    58561 atggtcaaaa tgaactgcgc ggcgatgcct gccggattgc tggaaagcga tctgtttggt
    58621 catgagcgtg gggcttttac cggtgccagc gcccagcgca ttggtcgttt tgaactggcg
    58681 gataaaagct ctctgttcct cgacgaagtg ggcgatatgc cactggagtt acagccgaag
    58741 ttgctgcgtg tattgcagga acaggagttt gaacgtctcg gcagcaacaa aatcattcag
    58801 acggacgtac gtttaattgc cgcgactaac cgcgatctga aaaaaatggt cgccgaccga
    58861 gagttccgta gcgatctcta ttaccgcctg aacgtattcc cgattcacct gccgccacta
    58921 cgcgagcgtc cggaagatat tccgctgctg gcgaaagcct ttaccttcaa aattgcccgt
    58981 cgtctggggc gcaatatcga cagcattcct gccgagacgc tgcgcacctt gagcaacatg
    59041 gagtggccgg gtaacgtacg cgaactggaa aacgtcattg agcgcgcggt attgctaaca
    59101 cgcggtaacg tgctacagct gtcattgcca gatattgttt taccggaacc tgaaacgccg
    59161 cctgccgcaa cggttgtcgc ccaggagggc gaagatgaat atcagttgat tgtgcgcgtg
    59221 ctgaaagaaa ccaacggcgt ggttgccggg cctaaaggcg ctgcgcaacg tctggggctg
    59281 aaacgcacga ccctgctgtc acggatgaag cggctgggaa ttgataaatc ggcattgatt
    59341 taactgcaaa ttgccggaca gatctgcctg tccggcatac tattcacgag gttttttcgg
    59401 acgatatttt tccggcagtt ctggcaccgg acgcttgtca tcgatgagat gacgcacggt
    59461 taagatcgga tgacgccaca gcattctcgg cccggcccaa cgcataatct gtttcatctc
    59521 ttcacgcttt gcaggctggt aacagtgcac cggacactgc ttacaggctg gtttctcttc
    59581 gccgaacaca catttatcca gccgcttttg cgcgtaaaca aacaacgcct cgtaatgctc
    59641 cggctccgct gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt ttttaatcgt
    59701 cagtttttca cgagagatac gcttgccgga catgctgcct ccatactctt aaggtgtatt
    59761 tatattacaa cttaatttga aaggggacta tggctctgaa ggaggaggtt tattcaaccg
    59821 acgcaatttt gcgtacttgc ttcataagca tcacgcaaaa gctgcaaaac agcatctttc
    59881 ccggaaccag catcaagaac tcgcagttcg cttcttcccc tgaaatgatt aactccggta
    59941 tcatgtgcgc cttatgtgat tacaacgaaa ataaaaacca tcacacccca tttaatatca
    60001 gggaaccgga cataacccca tgagtgcaat agaaaatttc gacgcccata cgcccatgat
    60061 gcagcagtat ctcaggctga aagcccagca tcccgagatc ctgctgtttt accggatggg
    60121 tgatttttat gaactgtttt atgacgacgc aaaacgcgcg tcgcaactgc tggatatttc
    60181 actgaccaaa cgcggtgctt cggcgggaga gccgatcccg atggcgggga ttccctacca
    60241 tgcggtggaa aactatctcg ccaaactggt gaatcaggga gagtccgttg ccatctgcga
    60301 acaaattggc gatccggcga ccagcaaagg tccggttgag cgcaaagttg tgcgtatcgt
    60361 tacgccaggc accatcagcg atgaagccct gttacaggag cgtcaggaca acctgctggc
    60421 ggctatctgg caggacagca aaggtttcgg ctacgcgacg ctggatatca gctccgggcg
    60481 ttttcgcctg agcgaaccgg ccgaccgcga aacgatggcg gcagagctgc aacgcactaa
    60541 tccggcggaa ctgctgtatg cagaagattt cgccgagatg tcgctgattg aaggccgtcg
    60601 cggcctgcgc cgtcgcccgc tgtgggagtt tgaaatcgac accgcgcgcc agcagttgaa
    60661 tctgcaattt ggcacccgcg atctggtcgg ttttggcgtc gagaacgcgc cgcgcggact
    60721 ttgtgctgcc ggttgtctgt tgcagtatgc gaaagatacc caacgcacga ccctgccgca
    60781 tattcgttct atcaccatgg aacgtgagca ggacagcatc attatggatg ccgcgacacg
    60841 tcgtaacctg gaaattactc agaacctggc gggcggtgcg gaaaatacgc tggcttctgt
    60901 gctcgactgc accgtcacgc cgatgggtag tcgtatgctg aaacgctggc tgcatatgcc
    60961 agtgcgcgat acccgcgtgt tgcttgagcg ccagcaaact attggcgcat tgcaggattt
    61021 caccgccgag ttgcagccgg tactgcgtca ggtcggcgac ctggaacgta ttctggcacg
    61081 tctggcttta cgaactgctc gcccacgcga tctggcccgt atgcgccacg ctttccagca
    61141 actgccggag ctgcgtgcgc agttagaaac tgtcgatagt gcaccggtac aggcgctacg
    61201 tgagaagatg ggcgagtttg ccgagctgcg cgatctgctg gagcgagcaa tcatcgacac
    61261 accgccagtg ctggtacgcg acggtggtgt tatcgcatcg ggctataacg aagagctgga
    61321 tgagtggcgc gcgctggctg acggcgcgac cgattatctg gagcgtctgg aagtccgcga
    61381 gcgtgaacgt accggcctgg acacgctgaa agttggcttt aatgcggtgc acggctacta
    61441 cattcaaatc agccgtgggc aaagccatct ggcacccatc aactacatgc gtcgccagac
    61501 gctgaaaaac gccgagcgct acatcattcc agagctaaaa gagtacgaag ataaagttct
    61561 cacctcaaaa ggcaaagcac tggcactgga aaaacagctt tatgaagagc tgttcgacct
    61621 gctgttgccg catctggaag cgttgcaaca gagcgcgagc gcgctggcgg aactcgacgt
    61681 gctggttaac ctggcggaac gggcctatac cctgaactac acctgcccga ccttcattga
    61741 taaaccgggc attcgcatta ccgaaggtcg ccatccggta gttgaacaag tactgaatga
    61801 gccatttatc gctaacccgc tgaatctgtc gccgcagcgc cgtatgttga ttattaccgg
    61861 cccgaacatg ggcggtaaaa gtacctatat gcgccagacc gcactgattg cgctgatggc
    61921 ctacatcggc agctatgtac cggcacaaaa agtcgagatt ggacctatcg atcgcatctt
    61981 tacccgcgta ggcgcggcag atgacctggc ttccgggcgc tcaaccttta tggtggagat
    62041 gactgaaacc gccaatattt tacataacgc caccgaatac agtctggtgt taatggatga
    62101 gatcgggcgt ggaacgtcca cctacgatgg tctgtcgctg gcgtgggcgt gcgcagaaaa
    62161 tctggcgaat aagattaagg cattgacgct gtttgccacc cactatttcg agctgaccca
    62221 gctaccggag aaaatggaag gcgtcgccaa cgtgcatctc gatgcgctgg agcacggaga
    62281 caccattgcc tttatgcata gcgtgcagga tggcgcggcg agcaaaagct acggcctggc
    62341 ggttgcagct ctggcaggcg tgccaaaaga ggtgattaag cgcgcacggc aaaaactgcg
    62401 tgagctggaa agcatttcgc cgaacgccgc cgctacgcaa gtggatggta cgcaaatgtc
    62461 tttgctgtca gtgccagaag aaacttcgcc tgcggtcgaa gcactggaaa atctcgaccc
    62521 ggattcactg actccgcgtc aggcgctgga gtggatttat cgcttgaaga gtctggtgta
    62581 ataacaattc ccgatagtct tttgctatcg ggaatattaa cgacaactga cgaatcaaat
    62641 aaaaataccc tgtataatag gaaagcttat tttacagggt aaaaccatgc catctacacg
    62701 ctatcaaaaa atcaataccc atcactatcg ccatatatgg gtcgttggtg atattcatgg
    62761 tgaatatcag ttattacaat cccgcttaca tcaactctct tttttccccg aaaccgactt
    62821 acttatttct gtcggcgata atattgatcg tgggccggag agtcttgacg tcctgcgcct
    62881 gctaaaccag ccctggttta tctccgttaa aggcaaccac gaagcaatgg cgctggatgc
    62941 gttcgcgacc ggcgatggca atatgtggct tgccagcggt ggtgactggt ttttcgattt
    63001 aaatgattca gagcaaaaag aagctacaga tctgttgctg aaattccatc accttccaca
    63061 tattattgaa atcactaacg acaacataaa atatgtcatc gcacatgcag attatccggg
    63121 gagtgaatat ctctttggta aagaaatagc ggagagcgaa ttactctggc ctgttgatcg
    63181 tgtgcagaaa tcgcttaatg gcgagttaca acaaataaac ggcgctgatt attttatatt
    63241 tggacatatg atgtttgata acattcagac gttcgctaac cagatttata ttgataccgg
    63301 cacgccgaaa agcgggcggc tgtcatttta taaaataaag tagtctcatg cttcttctgt
    63361 gaagcatgag taaccaaatg ttattgcagg ccattattca tttttcgcta ccagcaaaga
    63421 gagatcctgc ttcgccagcg cgcgactggc actctccggc aaaccgtcgt ctgtaataat
    63481 ctgatcaaac tcgcttaatg gtaacgccag ccatgtcgcc acctgaccat atttcgtcgc
    63541 atcacagacc aaaactcgct ggcggctggc actggcaatc gcccgtttca ccgtgacttt
    63601 atcttccgct ggcgtagaaa tcccccgcac actccatgac gatgcagaaa taaaagcctg
    63661 atcaatcatc aggctgcgca gcatggtcgc agcggcttcc ccgacacagg aacggttttc
    63721 ccgacacact gcaccgccag tgtgaataat tgtgcaatta ctgttgtcga gcaagtagtt
    63781 cgcaataacg aaatcgtttg tgaccacagt cagtgactcc atgtgaatca gatgctgtgc
    63841 tatcgctaac gtggtcgttc ccgcatccag atagatacaa cttcccggct gaacaagact
    63901 tgccgccagc ttgccaatag ccgctttttg cgtcattgcc agcgcagttt ttacctgatg
    63961 agaaggttca tgcgccacgc gtcccggaga ctggacgcct ccggacacca gcacaacggc
    64021 tccctgctgc tccagttttt gtaaatcccg acgaatggtc atatgtgaca cattcattct
    64081 gtccgttagt tcagcaatac tgacaatgcc tttttcagct accatctcaa ggatgatttg
    64141 gcgacgctct acgggtatca acttttgctc cttcctttgt cctgctgaca ttctacgcta
    64201 tttgcctgcg aaacgtgcgc ggcgcaacta acgcttagtt cacataaaat aacacacaat
    64261 gttaatttat gtgaatcagc tcaccatatc gttatcttcc agcgcttata ttcacaatat
    64321 caaacaaaat atcacttaaa ttaacaaggg gagcagatga aaacgggatc tgagtttcat
    64381 gtcggtatcg ttggcttagg gtcaatggga atgggagcag cactgtcatg tgtccgcgca
    64441 ggtctttcta cctggggcgc agacctgaac agcaatgcct gcgctacgtt gaaagaggca
    64501 ggtgcttgcg gggtttctga taacgccgcg acgtttgccg aaaaactgga cgctctgctg
    64561 gtgctggtgg tcaatgcggc ccaggttaaa caggtgctgt ttggtgaaac aggcgttgca
    64621 caacatctga aacccggtac ggcagtaatg gtttcttcca ctatcgctag tgctgatgcg
    64681 caagaaattg ctaccgctct ggctggattc gatctggaaa tgctggatgc gccagtttct
    64741 ggtggtgcag taaaagccgc taacggtgaa atgactgtca tggcctccgg tagcgatatt
    64801 gcctttgaac gactggcacc cgtgctggaa gccgttgccg gaaaagttta tcgcataggt
    64861 gcagaaccgg gactaggttc gaccgtaaaa attattcacc agttgttagc gggcgtacat
    64921 attgctgccg gagccgaagc gatggcactt gcagcccgtg cggggatccc gctggatgtg
    64981 atgtatgacg tcgtgaccaa tgccgccgga aattcctgga tgttcgaaaa ccggatgcgt
    65041 catgtggtgg atggcgatta caccccgcat tcagccgtcg atatttttgt taaggatctt
    65101 ggtctggttg ccgatacagc caaagccctg cacttcccgc tgccattggc ctcaacagca
    65161 ttgaatatgt tcaccagcgc cagtaacgca ggttacggga aagaagacga tagcgcagtt
    65221 atcaagattt tctctggcat cactctaccg ggagcgaaat catgatcaag attggcgtta
    65281 tcgccgatga ttttaccggc gcgacggata tcgccagttt tctggtggaa aacggtctac
    65341 caacggtaca aattaacggt gttccaacag gtaaaatgcc ggaagcaatc gacgcactgg
    65401 tgatcagcct gaaaacgcgc tcctgtccgg tggttgaagc cacacagcaa tcgctggcgg
    65461 ctctgagctg gttgcaacag caaggttgca aacagatcta tttcaaatac tgctctactt
    65521 tcgacagtac ggcgaaaggt aatatcggcc cggttaccga tgcattaatg gatgctctcg
    65581 acacgccgtt tacggtcttc tctccggccc tgccggtcaa cggacgtacg gtttatcagg
    65641 ggtatttgtt cgtaatgaat caactgctgg ccgaatccgg gatgcgccat catccggtaa
    65701 atcccatgac cgacagctat cttccccgtc tggttgaagc gcaatccaca gggcgctgcg
    65761 gcgtcgtttc ggcacatgtt ttcgaacaag gtgtggatgc cgttcgtcaa gagctggctc
    65821 gcttacagca agagggctac cgctacgcgg tgcttgatgc gctgaccgaa caccatctgg
    65881 aaattcaggg agaagccttg cgcgatgccc cactggtaac gggcggttct ggtctggcga
    65941 ttggcctggc ccggcagtgg gcgcaagaaa acggtaacca ggctcgcgaa gcagggcatc
    66001 cgctcgctgg gcgcggcgta gtgctctccg gttcatgctc tcaaatgacc aaccgccagg
    66061 tggcacatta ccgtcaaatt gcaccagccc gtgaagttga tgtggcacgc tgcctctcaa
    66121 ccgaaactct ggccgcttat gcacacgaac tggcagagtg ggttctgggc caggaaagtg
    66181 tacttgctcc actggttttt gccaccgcca gcactgacgc attggcagca attcaacagc
    66241 aatacggtgc acaaaaagcc agtcaggcag tagaaactct attttctcaa ctagcggcgc
    66301 ggttagcagc ggaaggcgtg acacgcttta ttgtcgcagg cggtgagacc tccggcgtag
    66361 tcacacagag cctgggcata aaagggtttc atattggccc aaccatttcc cccggcgtgc
    66421 cgtgggtaaa cgcactggat aagcctgtct cactcgccct taaatctggc aacttcggtg
    66481 atgaagcctt tttttcacga gcccaaagag agtttttatc atgagcgatt tcgcaaaagt
    66541 agagcagtct ttgcgagagg agatgacgcg gattgccagt tcattctttc agcgcggcta
    66601 tgcaaccggt tcggctggca atctgtcgct gcttttacct gacgggaatt tactggcgac
    66661 accgacaggt tcatgcctgg gcaatctcga tccgcagcgg ctttccaaag tcgccgcgga
    66721 tggcgaatgg ttaagtggtg acaaaccctc gaaagaggtg ctctttcatc tggcgctgta
    66781 tcgcaacaat ccgcgctgta aagcggtggt gcatttgcac agcacatggt cgacggcgct
    66841 ttcctgcctg caagggctgg acagcagcaa cgttattcgt ccgttcacac catacgtggt
    66901 gatgcggatg ggaaatgtcc cgctggtgcc ttattaccga ccgggcgata aacgcatcgc
    66961 acaggatctg gcggaactgg cagcagacaa tcaggctttt ttactggcaa atcatggccc
    67021 agtggtttgc ggtgaaagcc tgcaagaagc cgccaacaat atggaagagc tggaggaaac
    67081 ggcaaagctg atttttattc tcggtgaccg cccgatccgt tatctgaccg caggtgaaat
    67141 tgcggaatta aggagttaat gcaatgcctc gttttgcagc taatttatcc atgatgttca
    67201 ccgaagtgcc ttttattgaa cgcttcgccg cagcgcgaaa agccgggttc gatgctgtgg
    67261 aatttctgtt tccctatgac tactccaccc tgcaaatcca aaagcaactg gagcaaaacc
    67321 atctgacact ggcgctgttc aataccgctc ccggagatat taatgcgggg gaatggggat
    67381 tatccgccct tcccggacgt gagcacgaag cacacgcaga catcgacctg gcactggaat
    67441 atgcattggc gctcaattgt gaacaagtcc atgtgatggc aggcgtcgtg cccgctggcg
    67501 aagacgctga acggtaccgg gcagtattta tcgataatct ccgctacgct gccgaccgct
    67561 ttgcgctaca cggcaagcga atattagttg aagcactcag ccccgacgtt aagcctcatt
    67621 atctcttctc cagccagtat caggcactgg ctattgttga agaggttgcg cgagataacg
    67681 tgtttattca actagatact tttcatgcgc aaaaagtaga tggcaacctg acgcatttaa
    67741 tccgcgacta cgctgggaaa tatgcgcacg tacaaattgc cggactacct gatcgacatg
    67801 aaccggacga tggagaaatc aactatccgt ggctgttccg cctgttcgat gaggtgggat
    67861 atcagggctg gatcggttgt gaatataaac ctcgtggcct caccgaagaa gggcttggct
    67921 ggtttgacgc ctggcgctaa ttcgttaaat ccctaattac aacgtaccca tacatccccc
    67981 catctacgga tggggtaggt taactataac tttcagacag ggtttcccat gtccacaatt
    68041 acattgttat gcattgcgtt agctggcgta atcatgctgt tgctgctggt catcaaggca
    68101 aaggtacaac cattcgttgc tctgctcctc gtcagcctgt tagtcgcact tgcggcgggt
    68161 ataccggcgg gcgaagtggg taaagtgatg atcgccggga tgggcggtgt gcttggttcc
    68221 gtcactatta ttattggtct gggcgctatg ctggggcgta tgatcgaaca ctctggtggt
    68281 gcagagtcac tggctaatta tttcagtcgc aagttaggtg acaaacgaac tatcgctgcg
    68341 ctgactctgg cagcgttctt cctcggtatt cccgtcttct ttgatgtcgg ctttattatt
    68401 cttgcgccaa tcatttacgg ttttgccaag gttgccaaaa tatcgccact caaatttggc
    68461 ctgcctgtcg ctggaatcat gctaactgtt catgtggcgg taccgccgca tccaggccct
    68521 gtcgccgcag cggggttact ccacgcagac atcggctggc taaccatcat cggtattgcg
    68581 atttctattc ccgtagggat tgttggctac tttgcagcga aaataatcaa tacgcgccaa
    68641 tatgcgatgt cggtggaagt actggaacag atgcaactgg ctccggccag tgaggaaggc
    68701 gcaacaaaat taagcgataa aataaatcca ccgggcgtcg cgctggtcac ctcgctaatt
    68761 gttattccta tcgcgattat catggcgggt acggtttccg caacactgat gccgccttcg
    68821 catcccctgc ttggtacgct acagctgatc ggctcaccaa tggtagctct aatgattgcg
    68881 ctggtgctgg cattctggtt attggcttta cgtcgcggct ggagcttaca acacaccagc
    68941 gacattatgg gctcagccct tcccactgcg gcggtagtga ttttggttac tggtgctgga
    69001 ggggtatttg gcaaagtgct ggtggaatcg ggcgtcggca aagcccttgc caatatgcta
    69061 caaatgattg acctgcctct gttaccagcc gcatttatta tttcactggc tctgcgtgca
    69121 tcgcaggggt cagcaaccgt agcaatcctg acaaccggcg ggttactctc agaagcggtg
    69181 atgggattga atccgattca gtgcgtattg gtgacgctgg cagcctgctt tggtgggctt
    69241 ggcgcgtcac atattaatga ctcagggttc tggattgtga ccaaatatct ggggttgtcg
    69301 gtagcagacg gtctgaaaac ctggacagtg ttaacgacca ttctcggttt taccggattc
    69361 ttaattacct ggtgcgtatg ggcggtaatt tgaccttaat aaaaaggtcc gatgggcatc
    69421 ggacctttta tttaacgctt accttccacc agcagcggcg ggatgctcgg caccattggc
    69481 gcgtcatcaa tatctttctg cgtcatgcgg aacgcttcgg gataatgttc gcggctggta
    69541 cggcgcagcg attcggtatc gcgccaggta taaaggcaat gctggcactg atataccgtc
    69601 cagacatctt tcaccggcga tttcgccatc acttcaatct gttcatcggc acaacgtgga
    69661 caaatcatct tcttctcctt atttacgtgc agccagcata gcggtcagtt tttcagccca
    69721 ggctttggtt tccggtaaat ccaccaccgg ttggctgtag tgaccacggt tgtccggggc
    69781 gacaggcgta gtggcgtcga taatcagctt gtcggtgatc cccgcagggc ttgagcctgg
    69841 atcgagttcc agcacggaca tattcggcaa ctgcaccaaa tcccctgccg ggttcacttt
    69901 cgaggagagc gcccacatca cctgcggcag gttgaacggg tcaacgtctt catcgaccat
    69961 aatcaccatc ttcacgtagc ccagaccatg cggcgtggtc attgcgcgca ggcccaccgc
    70021 gcgggcaaag ccgccgtagc gttttttggt ggagataatc gccagcaggc catgggtgta
    70081 catggcgttt accgcctgca cttcagggaa ctcggctttc agctgctgat acagcggcac
    70141 gcaggtggct ggccccatca ggtagtcgat ttcggtccac ggcataccga ggtacagcga
    70201 ttcgaaaatc ggcctggtgc ggtaagagac tttatcgatg cgcaccacgg tcatgttacg
    70261 cccgccggag tagtgcccgg taaactcacc gaacggccct tcgatttcgc gtttacggct
    70321 ttcgatgacc ccttcgagga tcacttctga accccacggc acatcaaaac cggtcaacgg
    70381 ggcggtggcg atcgggtacg ggctttcacg cagcgcgcct gccatttcgt actcggactg
    70441 atcatatttc agcggcgtgg cccccatcag cgtgatgatc ggatcgttac cgagcgtgat
    70501 cgcaatcggc agatcttcac cgcgctcttc tgctttatgc agatgcaggg cgatatcgtg
    70561 catcggcacc ggttgcaggc cgagcttacg cttgcccttc acttccatgc ggtagatgcc
    70621 gacgttctgc ttgccgaagt tatccgggtc gagcggatcg cgggaaacca cgcacgcttt
    70681 gtcgagatag aaaccgccat cgccatcgtt taaacgaaac agcggcagga tatcgaacag
    70741 gttgatctcg tcgccatcaa cggtgttctg cgcccaggct ggattggcgc ggcgctccgg
    70801 ggcaatcggg aagttatccc agcggcggat aaactcatca atctgctttt taaccggggc
    70861 atttggcggc aggccgaggg aaatcgcgtg gttctgccag gaaccgatgg tgttcatcgc
    70921 cacgcgggca tcggtaaagc cgcgaatatt atcaaaccac agcgcgggcg cgccgtcgcc
    70981 gatacgcccg gtggcgttag ctgctgctgc cagatccggc tcggcgttca cttcttcgct
    71041 gattttcagt aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta aatcatcaaa
    71101 tgccattatt cattctcctg agaaaaattc cgggcctgcg gcaatccttg ccagcgcctg
    71161 gcgtggggat gttcgaggcc aaattgatcc agcacgcggg ctaccacatg gtggacaatg
    71221 tcatctaccg tttcgggatg gttataaaag gcaggcatcg gcggcaccat cgccacgccc
    71281 atgcgtgaaa gtgcgagcat attttcgaga tggatggtgc taagcggcat ttcacgcggc
    71341 accagcacca gtttgcggcc ttctttgagc acgacgtccg ccgcgcgccc taccaggcca
    71401 tcggcgtaac cagcgcggat accggcgagc gttttcatac tgcacggaat aacgatcatg
    71461 ccgtcggtac gaaaagaacc ggatgagatg atcgccgcct gatccgccgg gttatggctg
    71521 aagtctgcga gggcagcaac atcgcgagcg ctgtaaggcg tttccagttc aatggtggtt
    71581 ttcgcccact tcgacatcac cagatgagtc tcgacattcg gcatctcccg cagcgcttgc
    71641 agtaatgcca caccaagagg cgcaccggta gcccctgtca tcccgacgat cagtttcatt
    71701 gcttatcctc ttaattgttc gtatacgaac gttattatta acatactcct tcatttccac
    71761 agcggcaagg attgaaaagt aacaacatct tcttcgtcac taaaaaaccg ctatgatagc
    71821 gacagagaat tagcccggag tttacatggc gttacgaaat aaagcgttcc atcagttacg
    71881 acagcttttt cagcagcaca cggctcgctg gcagcacgag ttacctgacc tgaccaaacc
    71941 acagtacgcg gtgatgcgcg ccattgctga taaacctggt atcgaacagg tggcactgat
    72001 agaagcagct gtcagcacca aagcaacgtt agcagaaatg ttggcaagaa tggaaaatcg
    72061 tggcttagtc agacgtgaac atgaccccgc tgataagcgg cgacgctttg tctggttgac
    72121 tgccgaaggc gaaaagatac ttgctgcggc gatacccatt ggtgacagcg tggatgagga
    72181 atttttgggg cgtttgagtg gagaagagca ggagctgttt atgcagctgg tgcgcaagat
    72241 gatgaacgca tagggggcaa atttccggat gcgacgctat cgcgtcttat ccagactaca
    72301 aatacaccga ctcaccaacc gaaagaaaaa aggccagcct cgcttgagac tggcctttct
    72361 gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc gtttgcagga
    72421 tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca cgggtgaggc
    72481 caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa ccgaatcgac
    72541 gtgccagcac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg atgctctgct
    72601 tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg gccaggatgt
    72661 ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta atgcgctcgt
    72721 taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct gcgatctctt
    72781 ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc aggtaaacgt
    72841 tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc ataatcgccc
    72901 gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa ccacgttccg
    72961 ggtcaaactt ctctaccgcg cggatcagcc ccaggttgcc ctcttcgata aggtccagca
    73021 acgccagacc acgattgcca taacggcggg caatttttac caccagacgc aagttactct
    73081 cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca aaataaactt
    73141 cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc tgagtcgcgt
    73201 ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa tcgttatcac
    73261 tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc tcatcaaatt
    73321 ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg tggctcctac
    73381 ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt atcgctgcgg
    73441 caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat gcaagcgtgt
    73501 tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca cttcttgttg
    73561 ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat cattatgttt
    73621 gatgataatc agattaccgt agccgcgcag cgcgttaccg gcataaacaa cgcggccatc
    73681 tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct tgttgccccc
    73741 ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc aggtggagat
    73801 aggcgtactg gttgatgtac tgctgacagt tggctcggtt gtgcttgctg ttggtaccgt
    73861 tacaggcgct gtgaccgtgg tcgcagctgg cttgttgttc ggcaacattt tgttagcact
    73921 ctgttcaccc gaagattcag aatacgtaat tgtcggttgc gacgcaacag caacggtgga
    73981 attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg taatggcatt
    74041 tccgccagtg attggcgtac cggaagcatt acccacctgc aaggtctgac caacgttcag
    74101 cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt tgccagtaat
    74161 ccaggcgata tagaaaagtg tgtcgccttt tttcacggta taggtactgc cgctataact
    74221 gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt ccatctgtac
    74281 cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag cctgaatttg
    74341 tggctgctgt accggctgaa tttgcggttg ctgggctgta gacgtcgtcc ccattttcgg
    74401 cggcggcgta atcaacatac cagaattggt atttgcaggc gcattgccat taacggagct
    74461 gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg aaaccagtga
    74521 caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt atcccccagg
    74581 aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact gaacactgga
    74641 aaagatgttc acgatacgct gacctgcggc aaaataacca ggaaaaatcc aggtatttcc
    74701 tcacgtttta agccagctca cccttcacta aaggtacaaa gcgcacggcc tccacggtat
    74761 cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc tcctccccta
    74821 cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt ggaatttccg
    74881 gcggtgccgc cgtaacaatg atagcgtcaa acggcgcgcg tgcctgccaa ccttgccatc
    74941 catcgccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg cgacgcgcct
    75001 gccactgcaa acctttaatc cgttcgaccg agcaaacatg ctggacaaga tgcgccagga
    75061 ttgccgtttg atatcccgaa ccagtgccaa tttccagcac ccgcgactgc ggcgtcagct
    75121 cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc ccctgaccta
    75181 tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg aatttttcac
    75241 gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc gcacgtaatt
    75301 gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca cgctgtttaa
    75361 ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca gcggcgtgat
    75421 ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat cacatttacc
    75481 gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg ggatcacctg
    75541 atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt gatccaaggg
    75601 taaatccgga acgttaatat taagaatacg cccggtgcgc aacggctctt tacacagtgc
    75661 gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc cgtcaagcga
    75721 gacggcaagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta ccgtaccgga
    75781 ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa caatgtccgg
    75841 gcgcggacgc atcagagcat tcacgccaag atagacgcaa tcggtcgggg ttcccatttg
    75901 cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca gtgtcagaga
    75961 atttgaagcg ccgctgcggt tacgatcggg ggcgaccacc tgaacgtcag caaactcacg
    76021 caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat tactcagcaa
    76081 tatgcgcata atcacctgtt gtgttgataa gttccctgac aacgctggtt gcaaaactac
    76141 ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccaattc cagcttaatt
    76201 gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc accagtaaag
    76261 cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct tcacgctgag
    76321 ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta tcgttaacgc
    76381 gacgctgtaa ttccgccagt tcttcggtgg ttgcgacaaa ccagctacca cgtccggcta
    76441 attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg cgctcagcaa
    76501 caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt ttattgcgat
    76561 cgcgcaccgg agtattggtt tgcgcccagc gctgcgcccc ctgcaagttg ctaccgccaa
    76621 tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tacgcaaata tcgttcagac
    76681 gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag gcgtttcctt
    76741 tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc tggcagcctt
    76801 ccagttgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagcg cataaccact
    76861 gttccgtaac agcatgtttg tctttttgcc cagcgaagct gacttcacgg gcatgaattt
    76921 tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt ttgaggattc
    76981 taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc accaccacaa
    77041 agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg aggtaagtga
    77101 gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt cacaggcaat
    77161 cccttcccca cgtccggtaa atcccagttt ttccgtagta gtggctttca cgttaacatc
    77221 atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa tgtgcggcaa
    77281 catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat aacccttcgc
    77341 ctgaatacga cgccaggctt cgcgtagcag ctcgcggcta tcggcacctt taaatgccgg
    77401 atcggtatcc gggaacagct tgccgatatc ccccagcgcc gccgcgccaa gcaatgcatc
    77461 ggtcaacgca tggagcgcca cgtcgccatc agaatgcgcc agcaatcctt tttcgtaagg
    77521 aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcatgta cgtcaaaacc
    77581 gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta aaactcggcc
    77641 agtgccaaat cttccgggcg cgtgacttta atgttatccg cacggccttc gaccaactga
    77701 ggatggaatc cgcaatattc cagcgccgag gcttcgtcgg taatagtcgc gccttcattt
    77761 agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg cgtcagcgcg
    77821 tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg ttcggcacgt
    77881 ttcatagtat cgcgcactgg tgcggcgagg atcccccccg tgcggctggt ttcgctcaac
    77941 gccaacaatc gcgcgaggtc atcctgatgc aaacaaggac gagcggcgtc atgcaccaat
    78001 acccactgcg cgtcgccagc ggctttcaga cctgccagca cggaatcggc acgctcatca
    78061 ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc aaaacggcta
    78121 tcgccaggac ttatggcaat gacgactcgt ttcacccggg gatgcgccag cagcgcatgc
    78181 accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg acattccgtt
    78241 tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa atgagtggtt
    78301 gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg tgcgcgcttc
    78361 gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct gagttcatta
    78421 cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc ggcaaaaagt
    78481 tgatcgtttc gcgctttaag tttcgcgttt gtagcttgct gtgccgccac atcatcattg
    78541 acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg tagccagacc
    78601 agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa acggcatcat
    78661 catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg acaacgcggg
    78721 caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac ataatctcat
    78781 tattagttac ggtttgaatt atgaacagag gagacgggaa agtacaaatt agcccagtag
    78841 ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa caatactgta
    78901 gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg cgacgggcat
    78961 cagcgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag agccataaat
    79021 caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg caccaggcag
    79081 tgaccatgtg gtttcttcat cctcagtaag gctgtcgtta tttgttagtg taatgttatg
    79141 gctattacgc atatttgatc ctgttacttt gacgaaccgg gcatggaaac ccggtggtgt
    79201 ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt accaaatttg
    79261 ttactaattg ttcaccattg agatgaattt ctgccgattc aggcgcttcg taaacggaat
    79321 ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag ccttttggat
    79381 cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcgataaag cgcccttctc
    79441 ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat gcggtcagca
    79501 ctaccagtcc ggcttcaacc atcaaattcg ccacttcacc gacgcgacgg atattctctt
    79561 tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca ttatcgccat
    79621 ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc ccggcgaccg
    79681 ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga tgaccgtggt
    79741 gtagctcgcg ttgttgcaca gtgaccggat ggctatgcca gacgacgttt tcgtcatgca
    79801 gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt ggcgacgaac
    79861 cagagcattc aattccagtt cgaatgcact gaattcagat ggcgcagcag ttgcctggct
    79921 aactggctcg tgcaccatac cggcacccac ggtcacattg ctcaggcgat cgataaaaat
    79981 cagcccaccc gtcaccggat tttgttgata acgatctaac accagcggct cgtcaaaagt
    80041 gagatccacg aggccgatcc cattcagtgg caggttttca acttcacgct gggtaaggtt
    80101 attaatatca acctgatagc gaatgccatc aacacgcgcg cgcgtcttct taccggcaat
    80161 tttgatgtcg taactctgcc ctggagaaag cggctgttcc gccatccata ccacatccac
    80221 cgacgcgctc tgcaccgccg gtaacgcttc gtctgccgcc agcagcagat cgccacggct
    80281 gatgtcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaagg cttcttcgcg
    80341 atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg gcagcacttt
    80401 gacacgttgc ccgacttcca cgcgaccgga tgccagcgtt ccggcgtaac cacgaaaatc
    80461 gagattcggg cgattaacgt actgcaccgg gaagcgcatt ggctgagcat ccaccactcg
    80521 ctggatctcc acggtttcca gcacttcgag cagtgtcgga ccgctgtacc acggcatact
    80581 ttcactttgc gatgccacgt tgtcgccttc cagtgcagag agcggcacaa agcggatatc
    80641 cagattaccc ggcagctgcc cggcaaaggt caaataatct tcacgaatac gggtgaacgt
    80701 ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat gtttgctccc
    80761 caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt tacgggcatc
    80821 gatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc gggtgtactg
    80881 ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga aatagcggta
    80941 ggccacgtca atggtgatgc cctgttcgcg ctcagcttgc aggccgtcca ccagcagagc
    81001 cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca gcgatgagag
    81061 ctgatcttcg tagatttggc gggtatcgtg cagcagacga ccaatcagag tacttttgcc
    81121 gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt gcgcaatcat
    81181 ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtgcggtgt tcatcttaaa
    81241 aatacccctg acgttttttc agctccatag accccgcctg gtcgcggtca atcacgcggc
    81301 cctgacgttc actggtggtg gaaaccagca tctcttcgat gatttccggc agtgtttgtg
    81361 catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa cgcaccatcc
    81421 gttttttaat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc atcatcaaca
    81481 taccgtcgcg ttccagaacc ggacgttccg cagcaagata tagcggaaca atgtcgatat
    81541 tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc gggaagacgc
    81601 ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc gggcgctgat
    81661 ttttcggatc ccagcgatgg aagcggtcac ggaaagagta aattcgctct ttagcgcggg
    81721 atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat ttgttcagcg
    81781 cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg tgcacgaatg
    81841 gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagttcg cagccgtagg
    81901 ctttagcagt acgatcgcgg aactcataca tctcgcggaa tttccagccg gtatcgacat
    81961 gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga tgcagcatga
    82021 cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct gccgccacct
    82081 cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt cgtatttgat
    82141 ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc gactataggg
    82201 agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac ggaataacga
    82261 tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt ttaggaagat
    82321 ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa tttgcatggt
    82381 atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct ggcgctcggc
    82441 gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt tgctaatact
    82501 caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc tgcagaaatg
    82561 ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag tgatattcgg
    82621 acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagagctg gcataacgtg
    82681 acgggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat catcattatg
    82741 gcgcatctgg atacttacgc cccgctgagc gatgctgacg ccgatgccaa tctcggcggg
    82801 cttacgttac aaggaatgga tgataacgcc gcaggtttag gtgtcatgct ggaattggca
    82861 gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac cagcggcgaa
    82921 gaggaaggga aattaggcgc agagaattta ctcaagcgga tgagtgacac cgaaaagaaa
    82981 aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt gtatttcaac
    83041 agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc gctggcaatt
    83101 gcgcgtagtc atggaattgc cgcaacgacc aatccgggtt tgaataaaaa ttatccgaaa
    83161 ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc tgtactttcg
    83221 gtggaagcga ctaactggaa tcttgggaat aaggatggtt atcagcaacg cgcaaaaaca
    83281 cctgccttcc cggcgggaaa tagctggcat gacgtaagac tggataatca acaacatatt
    83341 gataaggcac ttcctggaag aatagaacgt cgctgccgtg atgttatgcg gataatgcta
    83401 ccgctggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga gctgggagtt
    83461 ctaccgcagg ggcggggaat tctaagtgat atccatcatc gcatccagtg cgcccggttt
    83521 atccccgctg acgcggggaa cacaaaacca aacttctcca taaattccat agccgcggtt
    83581 tatccccgct ggcgcgggga acacgagtct atcagcgaca ctaccggcaa tagcgacggt
    83641 ttatccccgc tggcgcgggg aacactcttc gcgggtaatc aatgatgatt cagtttccgg
    83701 tttatccccg ctggcgcggg gaacacgaac gcaatatcac ggcgttctgc ggcctcggcg
    83761 gtttatcccc gctggcgcgg ggaacaccaa aatattacga gcttcgtcag gccatggacc
    83821 ggtttatccc cgctggcgcg gggaacacgt cgggccgggt aagtgaacct gtcggttttc
    83881 cggtttatcc ccgctggcgc ggggaacaca cta
//
LOCUS       DAZFDP010000005        73592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFDP010000005 DAZFDP010000000
VERSION     DAZFDP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHJ1G_000389"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHJ1G_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4762303.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHJ1G_000390"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHJ1G_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4762304.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHJ1G_000391"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHJ1G_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4762305.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHJ1G_000392"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHJ1G_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4762306.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHJ1G_000393"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHJ1G_000393"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4762307.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHJ1G_000394"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHJ1G_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4762308.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHJ1G_000395"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHJ1G_000395"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4762309.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHJ1G_000396"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHJ1G_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4762310.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHJ1G_000397"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHJ1G_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4762311.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHJ1G_000398"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHJ1G_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4762312.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHJ1G_000399"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHJ1G_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4762313.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="ACHJ1G_000400"
     tRNA            complement(13705..13781)
                     /locus_tag="ACHJ1G_000400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHJ1G_000401"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHJ1G_000401"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4762314.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHJ1G_000402"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHJ1G_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4762315.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHJ1G_000403"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHJ1G_000403"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHJ1G_000404"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHJ1G_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4762316.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHJ1G_000405"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHJ1G_000405"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4762317.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHJ1G_000406"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHJ1G_000406"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4762318.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHJ1G_000407"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHJ1G_000407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4762319.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHJ1G_000408"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHJ1G_000408"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4762320.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHJ1G_000409"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHJ1G_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4762321.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHJ1G_000410"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHJ1G_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4762322.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHJ1G_000411"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHJ1G_000411"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4762323.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHJ1G_000412"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHJ1G_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4762324.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHJ1G_000413"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHJ1G_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4762325.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHJ1G_000414"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHJ1G_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4762326.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHJ1G_000415"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHJ1G_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4762327.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHJ1G_000416"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHJ1G_000416"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4762328.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHJ1G_000417"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHJ1G_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4762329.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHJ1G_000418"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHJ1G_000418"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4762330.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHJ1G_000419"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHJ1G_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4762331.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHJ1G_000420"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHJ1G_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4762332.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHJ1G_000421"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHJ1G_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4762333.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHJ1G_000422"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHJ1G_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4762334.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHJ1G_000423"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHJ1G_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4762335.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHJ1G_000424"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHJ1G_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4762336.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHJ1G_000425"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHJ1G_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4762337.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHJ1G_000426"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHJ1G_000426"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4762338.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHJ1G_000427"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHJ1G_000427"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4762339.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHJ1G_000428"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHJ1G_000428"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4762340.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHJ1G_000429"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHJ1G_000429"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4762341.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHJ1G_000430"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHJ1G_000430"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4762342.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHJ1G_000431"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHJ1G_000431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4762343.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHJ1G_000432"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHJ1G_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4762344.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHJ1G_000433"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHJ1G_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4762345.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHJ1G_000434"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHJ1G_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4762346.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHJ1G_000435"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHJ1G_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4762347.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHJ1G_000436"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHJ1G_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4762348.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHJ1G_000437"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHJ1G_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4762349.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHJ1G_000438"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHJ1G_000438"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4762350.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHJ1G_000439"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHJ1G_000439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4762351.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHJ1G_000440"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHJ1G_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4762352.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSITEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50070..54530
                     /gene="gltB"
                     /locus_tag="ACHJ1G_000441"
     CDS             50070..54530
                     /gene="gltB"
                     /locus_tag="ACHJ1G_000441"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4762353.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54543..55961
                     /gene="gltD"
                     /locus_tag="ACHJ1G_000442"
     CDS             54543..55961
                     /gene="gltD"
                     /locus_tag="ACHJ1G_000442"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4762354.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHJ1G_000443"
                     /pseudo
     CDS             56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHJ1G_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHJ1G_000444"
     CDS             complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHJ1G_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4762355.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHJ1G_000445"
     CDS             complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHJ1G_000445"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4762356.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHJ1G_000446"
     CDS             complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHJ1G_000446"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4762357.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHJ1G_000447"
     CDS             complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHJ1G_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4762358.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHJ1G_000448"
     CDS             complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHJ1G_000448"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4762359.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHJ1G_000449"
     CDS             complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHJ1G_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4762360.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            63108..64475
                     /gene="dcuC"
                     /locus_tag="ACHJ1G_000450"
     CDS             63108..64475
                     /gene="dcuC"
                     /locus_tag="ACHJ1G_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4762361.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(64518..65015)
                     /gene="sspB"
                     /locus_tag="ACHJ1G_000451"
     CDS             complement(64518..65015)
                     /gene="sspB"
                     /locus_tag="ACHJ1G_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4762362.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(65021..65659)
                     /gene="sspA"
                     /locus_tag="ACHJ1G_000452"
     CDS             complement(65021..65659)
                     /gene="sspA"
                     /locus_tag="ACHJ1G_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4762363.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(66054..66446)
                     /gene="rpsI"
                     /locus_tag="ACHJ1G_000453"
     CDS             complement(66054..66446)
                     /gene="rpsI"
                     /locus_tag="ACHJ1G_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4762364.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(66462..66890)
                     /gene="rplM"
                     /locus_tag="ACHJ1G_000454"
     CDS             complement(66462..66890)
                     /gene="rplM"
                     /locus_tag="ACHJ1G_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4762365.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(67109..68236)
                     /gene="zapE"
                     /locus_tag="ACHJ1G_000455"
     CDS             complement(67109..68236)
                     /gene="zapE"
                     /locus_tag="ACHJ1G_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4762366.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            68430..68828
                     /gene="zapG"
                     /locus_tag="ACHJ1G_000456"
     CDS             68430..68828
                     /gene="zapG"
                     /locus_tag="ACHJ1G_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4762367.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            68982..70349
                     /gene="degQ"
                     /locus_tag="ACHJ1G_000457"
     CDS             68982..70349
                     /gene="degQ"
                     /locus_tag="ACHJ1G_000457"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4762368.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            70439..71506
                     /gene="degS"
                     /locus_tag="ACHJ1G_000458"
     CDS             70439..71506
                     /gene="degS"
                     /locus_tag="ACHJ1G_000458"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4762369.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(71569..72507)
                     /gene="mdh"
                     /locus_tag="ACHJ1G_000459"
     CDS             complement(71569..72507)
                     /gene="mdh"
                     /locus_tag="ACHJ1G_000459"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4762370.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            72942..73412
                     /gene="argR"
                     /locus_tag="ACHJ1G_000460"
     CDS             72942..73412
                     /gene="argR"
                     /locus_tag="ACHJ1G_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4762371.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        18046 a        19793 c        18170 g        17583 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
     1681 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
     1741 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
     5161 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gttgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcatcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 tgtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggtaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc tattttaatt tcagtcattt aataaagaat tttgcgctaa agcacatttc
    49801 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    49861 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    49921 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga
    49981 cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    50041 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    50101 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    50161 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    50221 ccggcgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    50281 aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctattc ctgaataaag
    50341 atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct
    50401 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    50461 tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc
    50521 gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    50581 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    50641 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    50701 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    50761 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    50821 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    50881 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    50941 tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc gcctggcaga
    51001 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    51061 agccgtggga tggcccggcg ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc
    51121 tcgaccgtaa cggtctgcgt ccggcacgct acgtcattac caaagacaag ctcatcacct
    51181 gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg
    51241 tcgggccagg cgaactgatg gtgatcgaca cccgcagtgg gcgtattctg cactcggcag
    51301 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    51361 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg
    51421 acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag ctggactccg
    51481 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    51541 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    51601 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    51661 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    51721 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    51781 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    51841 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    51901 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    51961 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    52021 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    52081 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac acccatgcga
    52141 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    52201 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    52261 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    52321 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    52381 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    52441 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    52501 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    52561 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    52621 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    52681 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    52741 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    52801 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    52861 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    52921 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    52981 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    53041 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    53101 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    53161 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    53221 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    53281 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    53341 gcttcggcac tggcccgatg gtggcgctcg gctgtaaata tctacgtatt tgccatctga
    53401 acaactgcgc aacgggtgta gcaactcagg atgacaaact gcgtaagaac cactatcacg
    53461 gcctgccgtt caaggttacc aattactttg aatttattgc ccgtgaaacc cgcgagctga
    53521 tggcacagct gggcgtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag
    53581 aactggacgg tttcaccgcc aaacagcaaa aactggcgct gtcgaagctg ctggagaccg
    53641 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccaccg tttgataacg
    53701 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    53761 aaaccttctg gttcgatatt cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct
    53821 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    53881 acggcaccgc aggccagagt ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga
    53941 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    54001 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    54061 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    54121 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    54181 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    54241 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    54301 tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    54361 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    54421 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    54481 tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    54541 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac
    54601 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg
    54661 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    54721 cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt atttttgaag
    54781 cggcggaact gtcgcaccag accaaccccc tgccggaagt ttgcggacga gtctgcccgc
    54841 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    54901 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    54961 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    55021 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    55081 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    55141 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    55201 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    55261 ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag
    55321 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    55381 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    55441 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    55501 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    55561 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggtaaagtca
    55621 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    55681 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    55741 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    55801 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    55861 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    55921 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    55981 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    56041 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    56101 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    56161 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcatctg gtcgatagcg
    56221 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    56281 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    56341 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    56401 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    56461 tgtctggcga attaacacct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    56521 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    56581 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    56641 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcaaca ggcgatacgc
    56701 gatccctttg tgctcgaatt tctggaattg aaagatgaat attcagaatc tgatttcgag
    56761 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    56821 gttggtcggc agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    56881 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    56941 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    57001 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    57061 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    57121 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    57181 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    57241 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    57301 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    57361 attcccggtt ttaagatgat ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca
    57421 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    57481 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    57541 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    57601 tcgtaacgac ccggcgcttt ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc
    57661 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    57721 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg
    57781 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    57841 gatacccttc tgccagccca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    57901 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc agctgctgcg
    57961 cctgctcgtc gccctgcccg gcgcgtgtga aaatagttct cgcatccgcg cccgccagtt
    58021 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcctccacg caacccgtgc
    58081 gtccacagcc acagaccggg ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca
    58141 gaccgccagg gccggtaagc agtttgccgc cgctcactac accgccgccg acgccagtgg
    58201 aaacggtgat aaagaccata tcggttatat cgccttccag cgcctgatac tctgcccatg
    58261 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    58321 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgct agcaaactgc
    58381 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    58441 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    58501 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    58561 cgccgatatc aatcgccagc gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    58621 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    58681 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcaccctaca
    58741 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    58801 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    58861 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    58921 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    58981 gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta ccggagaatc
    59041 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    59101 cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc
    59161 catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct ggcaggagac
    59221 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    59281 tcccttagcg aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact
    59341 aaatcgtttt tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg
    59401 tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga
    59461 ccaatcagca ggatcaccac aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc
    59521 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcgcc gcccaatgca
    59581 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    59641 atcagtttcg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    59701 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat caacagctgc
    59761 gaggccaaca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagccg
    59821 ccgacacagc accccaccgc agccccaaag ccgctaaaga acagcacatt ggcgacagta
    59881 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    59941 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta
    60001 cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    60061 acgatcgcgg cattttgcag gttaccggcg aagcaaaacc acagcgcagt agccgccgcc
    60121 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc taccatcgtg
    60181 cgtactggtg ctttacctgc atgtttctct ttccagtctt ccgcttccgg gatgttttta
    60241 cgcagccaga gagcaaagat gataggcaaa atgccgataa agaacagcgc acgccagccc
    60301 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    60361 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    60421 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    60481 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    60541 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    60601 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    60661 ccgaattcac cttgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    60721 agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcacgatt gagatggcga
    60781 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    60841 acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    60901 ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    60961 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    61021 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    61081 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    61141 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactaccgat
    61201 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    61261 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaagtctg
    61321 tttcagcgca ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt
    61381 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat
    61441 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    61501 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    61561 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    61621 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    61681 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    61741 cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg
    61801 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    61861 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    61921 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    61981 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    62041 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    62101 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    62161 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    62221 gaatgaatgc cgcgttgtta tccagcgact gactgttaat ttccagtgct tttgccagca
    62281 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccaggctg gattcaaaga
    62341 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    62401 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    62461 cgttgttgat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag
    62521 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    62581 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    62641 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    62701 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    62761 gacatcctat cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca
    62821 tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa cgcagataaa
    62881 aacatcccgt ttgaattatt tataagacta ttcacgagca ttatgaatat tatgaatgtg
    62941 ttcttacaaa ataatcataa gcgcatattt tttaatgaaa aatcacctca cctacaatta
    63001 aaaacacgac atccgcacca taaatagact tgcaaaaaat ataacatcat tgttttcaat
    63061 ctgccgttta tgggattgac cgttttcttt tgacacggag ttcaacaatg ttcggcataa
    63121 ttatatctgt catcgtatta attacgatgg gctatttgat cctgaaaaac tacaaacctc
    63181 aggtggtgct ggctgccgca ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg
    63241 gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt tataatgaaa
    63301 tcctgcgcat gctgtccaac cgcattgccg gattggggct gtcgattatg gcagtgggcg
    63361 gttatgcccg ctacatggag cgcacaggag ccagtcgcgc gatggtgagc ttgctaagcc
    63421 gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac gtcatcggcc
    63481 aaatcatggc gcagtttatt accagcgcct ccggcctggg tatgttgctg atggtcacct
    63541 tatttccgac gctggtgagt ctgggagtaa gtcgcctctc tgcggtggcg gttatcgcaa
    63601 ccacgatgtc cattgagtgg gggattctgg aaacgaactc catttttgca gcccaggtcg
    63661 cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc tcttgcgtca
    63721 ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa
    63781 atatcaatca cgaacaggca gagctaaaag ctctcgataa tgtcccgccg ctctattacg
    63841 ccattttacc tgtgatgccg ttaatcttga tgctcggctc gctgttcctc gcccacatcg
    63901 ggctgatgca gtcagaactg catctggtgg cggtgatgtt actgagtttg acggtgacga
    63961 tgtttgttga gttcttccgc aagcataact tgcgcgaaac aatggacgat gtgcaggcgt
    64021 tttttgacgg catgggtacg cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa
    64081 tatttgcgaa aggcttaacg acgattggca ctgttgatgc ggttatcagg ggtgcggagc
    64141 attctggtct gggcggtatt ggcgtgatga ttattatggc gctagtcatt gccatttgtg
    64201 ccattgtgat gggctctggc aatgcgccgt ttatgtcatt tgccagtctt attccgaata
    64261 tcgcagccgg actacatgta ccagcggttg taatgattat gccgatgcat tttgccacga
    64321 cgctagcgcg cgcggtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
    64381 gcgtttcgcc ttttgcggtg gtgaagcgaa cagcgatccc catggcagtc ggtttcgtgg
    64441 tgaatatgat tgccacaatc acgctatttt attaagccat taaaagacaa aacaggccac
    64501 cagggcctgt ttttgtatta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc
    64561 tgcggaggtt catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata
    64621 accgacataa cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct
    64681 tcatcgtagg cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata
    64741 gccagcacgg cagccagcgg cacagaaacc tgacgcggaa tgccgccaaa gcgcgcgtta
    64801 aagcgtacct catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt
    64861 acgatttgcc cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc
    64921 accaccaagt gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga
    64981 tagggacgac gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca
    65041 tttcacgttc tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca
    65101 tatagccttt cagctctttc gcacccgggc cgctgaactc gatgcccagt tgcggcagac
    65161 gccacagcag cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg
    65221 gcttctgacc gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg
    65281 catctgcttc agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt
    65341 cgatgcgatg catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg
    65401 gcggatgcgg gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca
    65461 gctcacgatc caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag
    65521 gcggattgtc cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa
    65581 tgcggacctg atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt
    65641 tgttggcagc gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc
    65701 caccaggtgg ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa
    65761 atcaaacaaa aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca
    65821 gattttgcga cctttgtggt gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag
    65881 tctgaaatag ttgacttagt cccttattgg cgatgtggtt tttgttttgc ctgtctgtca
    65941 gacggtagca aaaagcaact ttccaatttt tacgctgatt cagaatttaa ccataaaaaa
    66001 acccgccgaa gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt
    66061 ttggagaact gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga
    66121 cgagcgtcac gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat
    66181 tccatcagag cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca
    66241 cctttaacgg tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga
    66301 cgaactacca tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt
    66361 acgattttac cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca
    66421 gtgccgtagt attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc
    66481 ggttgctgtg ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata
    66541 gcacgaccca gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca
    66601 ggacggcgag caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg
    66661 tggtgatagt acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc
    66721 agaacgatga tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg
    66781 cgcaggcgac gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca
    66841 acataccagt cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta
    66901 cccaataaat agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac
    66961 gacaaagtcc ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg
    67021 gaaaaaaact ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc
    67081 gacccctttt tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact
    67141 cttcgctttg catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat
    67201 cgccctgata aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct
    67261 cgtaaaactc atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca
    67321 taactggtac atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat
    67381 gctgactgcg ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga
    67441 cgcccattgt cgccaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcg
    67501 cccccgccag cgccaaccat agtttatcca tctgcgcccg ggtttcatcg ttgagtggcg
    67561 aaagccacag atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt
    67621 tcattacatc acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca
    67681 ggccatttcg ataaagttca tccggcggaa tatttgacgt cgctaccagg gtaatgccgc
    67741 gggcgaacag ggctttcatc agaccgccaa gtagcatggc gtcggtaata tcagaaacaa
    67801 aaaattcgtc aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca
    67861 gcggatcggt ctgcccctgt aaggcagtta gctcttcgtg cacccgcagc ataaaacggt
    67921 gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc
    67981 aggttttccc gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg
    68041 tgtcttcgcg tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg
    68101 ctggtggcct gctattgatg agttcttgat aaataatttc caggcggctg acggcctctt
    68161 tttgaacgtc gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg
    68221 gggtaacgct ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac
    68281 ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt
    68341 ggaattaacg gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct
    68401 acggaaacaa aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag
    68461 tcgtcggcat cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc
    68521 aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag
    68581 agctggttag ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc
    68641 gccagctgta tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg
    68701 aagcaaaccc gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg
    68761 tgcagatgcc tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc
    68821 gcgactaatt tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact
    68881 atcatttaat ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt
    68941 tgaatctctt ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaacccagc
    69001 tgttgagtgc attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg
    69061 cgtcgattcc aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg
    69121 aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga
    69181 aaatcccgga agaattcaaa aagttttttg gtgatgattt accggatcaa cctgcacaac
    69241 ccttcgaagg tttaggttcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca
    69301 acaaccatgt gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt
    69361 ttgatgcaaa actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa
    69421 acccgagcaa attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt
    69481 ttgccgtagc ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt
    69541 ccgcattagg ccgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg
    69601 cttccattaa ccgcggtaac tccggcggtg cactattaaa ccttaacggt gagttaattg
    69661 gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc
    69721 ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg
    69781 gtttgttagg catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg
    69841 acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcaaaagcgg
    69901 gcgtcaaagc gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg
    69961 agttgcgctc tcgtatcgcg actacagagc cgggcacgaa agtgaagctt ggcctgctgc
    70021 gtaacggcaa accactggaa gtagaagtga cgcttgatac cagcacctct tcgtcggcca
    70081 gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag
    70141 atggcggcaa aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg
    70201 gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg
    70261 aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggta
    70321 atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg
    70381 atgtccggtt aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat
    70441 gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt
    70501 tgccatgcct tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga
    70561 tgagacgcct gccagttata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt
    70621 ttacaaccgt ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc
    70681 cggtgtaatc atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc
    70741 cgatcagatc atcgtcgcct tacaggatgg acgtgtattt gaagcattgc tggtgggatc
    70801 tgactctcta accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat
    70861 tccgatcaat gcacgtcgcg taccgcacat tggcgacgtg gtactggcga tcggtaaccc
    70921 gtacaacctc gggcagacca ttacccaggg gattattagt gctacgggtc gaatcggtct
    70981 gaacccgacc gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc
    71041 tggcggcgcg ctggtgaact cgctgggaga actgatgggc attaacacgc tgtcgtttga
    71101 taagagtaac gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc
    71161 aaccaaaatt atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggtat
    71221 cggcggacgc gagatcgcac cactgcacgc gcagggcggt ggtatagatc aactgcaagg
    71281 gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa
    71341 cgatctgatt atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca
    71401 ggtggcagaa attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca
    71461 gttaacgctg caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa
    71521 acaaaaaacc ggagtctgtg ctccggtttt ttattattcg ctaattaatt acttattaac
    71581 gaactcttcg cccagggcga tatctttctt cagcgtatcc agcatacctt ccagtgcgct
    71641 ctgttcaaat gcgctcaggg taccgataga tttacgctct tccacgccgt ttttacccag
    71701 cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg ccttcaacgt aggcacattc
    71761 gacaacgcct tgttcgccct gcagtgcacg aaccagagac agaccaaaac gtgcagctgc
    71821 ctggcccata gacagggttg cagacccacc accggctttc gcttcaacca cttcagtacc
    71881 tgcgttctgg atacgtttgg tcagatcagc cacttcctgc tcggtaaaac taacgccagg
    71941 aacctgtgac agcagcggca gaatggtaac accagagtga ccgccaataa ccggcacttc
    72001 aacttcgcct ggctgtttgc ctttcagttc cgcaacaaaa gtgttggaac gaatgatatc
    72061 cagcgtggta acgccgaaca gtttgttttt gtcataaaca ccggcttttt tcagcacttc
    72121 agcagcaatc gcaactgtgg tgttaaccgg gttagtgata ataccaatgc acgctttcgg
    72181 gcaggttttc gcaacttgct gtaccaggtt tttcacgatg ccggcgttaa cgttaaacag
    72241 gtcggaacga tccatacccg gtttacgcgc tacacctgca gagataagaa cgacatctgc
    72301 gccttccagc gccggagtcg catcttcacc agaaaaacct ttgattttca cagcagtagg
    72361 gatatggctc agatcgacag ccacaccggg agtcactgga gcgatatcat acagagagag
    72421 ttctgaacct gaaggcagtt gggtttttaa cagtagtgca agcgcctggc caataccgcc
    72481 agcagcgccg aggactgcga ctttcatcct aaactcctta ttatattgat aaactaagat
    72541 atgttgctcc gctgccgcga ccttaatcca caaaattgcc gtttacaatg accacatctc
    72601 aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta attgccgtaa tcaggaacct
    72661 aacttacgta attaacagtc gcttatgcat tagcgcaaca ttccaacagg tggtgacaat
    72721 ataccctacc gttcagccaa aacaacatca atttgataac aattaattta cttttaaaca
    72781 gaatttgcat gccgtgacgc aggcatgttt ctcaataacg aaatttgata aaatcccgct
    72841 ctttcataac attatttcag ccttcttcag ggctgactgt ttgcataaaa attcatctgt
    72901 atgcacaata atgttgtatc aaccaccata tcgggtgact tatgcgaagc tcggctaagc
    72961 aagaagaact agttaaagca tttaaagcat tacttaaaga agagaaattt agctcccagg
    73021 gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa tattaatcag tctaaagtct
    73081 cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa tgccaaaatg gaaatggttt
    73141 actgcctgcc agctgaactg ggtgtaccaa ctacctccag tccattgaag aatctggtgc
    73201 tggatatcga ctacaacgat gcagttgtcg tgattcatac cagccctggc gcggcgcagt
    73261 taattgctcg cctgctggac tcactgggca aagcagaagg tattctgggc accatcgctg
    73321 gcgatgacac catctttacc acccctgcta acggtttcac cgtcaaagac ctgtacgaag
    73381 caattttaga gctgttcgac caggagcttt aatctctgcc ccgtcgcttc tggcgtcggg
    73441 gaaaatgttg cttatccctc tcattcccct tctttctcct gcgatacatt taacgaatag
    73501 tgcgttttac cgcgacacat cattcacaaa atgaatacat acggtaaaaa aagcacatta
    73561 tgcaaaattc attatctaat tgaaaatact at
//
LOCUS       DAZFDP010000006        70740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFDP010000006 DAZFDP010000000
VERSION     DAZFDP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            138..266
                     /gene="rhuM"
                     /locus_tag="ACHJ1G_000461"
                     /pseudo
     CDS             138..266
                     /gene="rhuM"
                     /locus_tag="ACHJ1G_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            695..1879
                     /gene="setC"
                     /locus_tag="ACHJ1G_000462"
     CDS             695..1879
                     /gene="setC"
                     /locus_tag="ACHJ1G_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO4762372.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1990..2913
                     /gene="yicL"
                     /locus_tag="ACHJ1G_000463"
     CDS             1990..2913
                     /gene="yicL"
                     /locus_tag="ACHJ1G_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4762373.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2917..3735)
                     /gene="nlpA"
                     /locus_tag="ACHJ1G_000464"
     CDS             complement(2917..3735)
                     /gene="nlpA"
                     /locus_tag="ACHJ1G_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4762374.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3957..4250
                     /gene="yicS"
                     /locus_tag="ACHJ1G_000465"
     CDS             3957..4250
                     /gene="yicS"
                     /locus_tag="ACHJ1G_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4762375.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(4291..5481)
                     /gene="nepI"
                     /locus_tag="ACHJ1G_000466"
     CDS             complement(4291..5481)
                     /gene="nepI"
                     /locus_tag="ACHJ1G_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4762376.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5692..6144)
                     /gene="yicN"
                     /locus_tag="ACHJ1G_000467"
     CDS             complement(5692..6144)
                     /gene="yicN"
                     /locus_tag="ACHJ1G_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4762377.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(6197..7531)
                     /gene="adeQ"
                     /locus_tag="ACHJ1G_000468"
                     /gene_synonym="yicO"
     CDS             complement(6197..7531)
                     /gene="adeQ"
                     /locus_tag="ACHJ1G_000468"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO4762378.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7706..9472
                     /gene="adeD"
                     /locus_tag="ACHJ1G_000469"
     CDS             7706..9472
                     /gene="adeD"
                     /locus_tag="ACHJ1G_000469"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4762379.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(9517..10908)
                     /gene="uhpT"
                     /locus_tag="ACHJ1G_000470"
     CDS             complement(9517..10908)
                     /gene="uhpT"
                     /locus_tag="ACHJ1G_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4762380.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(11164..12483)
                     /gene="uhpC"
                     /locus_tag="ACHJ1G_000471"
     CDS             complement(11164..12483)
                     /gene="uhpC"
                     /locus_tag="ACHJ1G_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4762381.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(12493..13995)
                     /gene="uhpB"
                     /locus_tag="ACHJ1G_000472"
     CDS             complement(12493..13995)
                     /gene="uhpB"
                     /locus_tag="ACHJ1G_000472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4762382.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13995..14585)
                     /gene="uhpA"
                     /locus_tag="ACHJ1G_000473"
     CDS             complement(13995..14585)
                     /gene="uhpA"
                     /locus_tag="ACHJ1G_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4762383.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14660..14950)
                     /gene="ilvN"
                     /locus_tag="ACHJ1G_000474"
     CDS             complement(14660..14950)
                     /gene="ilvN"
                     /locus_tag="ACHJ1G_000474"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4762384.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14954..16642)
                     /gene="ilvB"
                     /locus_tag="ACHJ1G_000475"
     CDS             complement(14954..16642)
                     /gene="ilvB"
                     /locus_tag="ACHJ1G_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4762385.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16748..16846)
                     /gene="ivbL"
                     /locus_tag="ACHJ1G_000476"
     CDS             complement(16748..16846)
                     /gene="ivbL"
                     /locus_tag="ACHJ1G_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4762386.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            17411..17500
                     /gene="tisB"
                     /locus_tag="ACHJ1G_000477"
     CDS             17411..17500
                     /gene="tisB"
                     /locus_tag="ACHJ1G_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4762387.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(17763..17837)
                     /gene="ysdE"
                     /locus_tag="ACHJ1G_000478"
     CDS             complement(17763..17837)
                     /gene="ysdE"
                     /locus_tag="ACHJ1G_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4762388.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            17919..19103
                     /gene="emrD"
                     /locus_tag="ACHJ1G_000479"
     CDS             17919..19103
                     /gene="emrD"
                     /locus_tag="ACHJ1G_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4762389.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(19111..19608)
                     /gene="yidF"
                     /locus_tag="ACHJ1G_000480"
     CDS             complement(19111..19608)
                     /gene="yidF"
                     /locus_tag="ACHJ1G_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4762390.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(19605..19967)
                     /gene="yidG"
                     /locus_tag="ACHJ1G_000481"
     CDS             complement(19605..19967)
                     /gene="yidG"
                     /locus_tag="ACHJ1G_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4762391.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(19957..20304)
                     /gene="yidH"
                     /locus_tag="ACHJ1G_000482"
     CDS             complement(19957..20304)
                     /gene="yidH"
                     /locus_tag="ACHJ1G_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4762392.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            20414..20863
                     /gene="yidI"
                     /locus_tag="ACHJ1G_000483"
     CDS             20414..20863
                     /gene="yidI"
                     /locus_tag="ACHJ1G_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4762393.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(20910..22403)
                     /gene="yidJ"
                     /locus_tag="ACHJ1G_000484"
     CDS             complement(20910..22403)
                     /gene="yidJ"
                     /locus_tag="ACHJ1G_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4762394.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(22400..24115)
                     /gene="yidK"
                     /locus_tag="ACHJ1G_000485"
     CDS             complement(22400..24115)
                     /gene="yidK"
                     /locus_tag="ACHJ1G_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4762395.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            24282..25175
                     /gene="yidL"
                     /locus_tag="ACHJ1G_000486"
     CDS             24282..25175
                     /gene="yidL"
                     /locus_tag="ACHJ1G_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4762396.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(25172..25986)
                     /locus_tag="ACHJ1G_000487"
                     /pseudo
     CDS             complement(25172..25986)
                     /locus_tag="ACHJ1G_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25986..27602)
                     /locus_tag="ACHJ1G_000488"
     CDS             complement(25986..27602)
                     /locus_tag="ACHJ1G_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4762397.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            27898..28614
                     /gene="yidP"
                     /locus_tag="ACHJ1G_000489"
     CDS             27898..28614
                     /gene="yidP"
                     /locus_tag="ACHJ1G_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4762398.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(28611..30272)
                     /gene="yidE"
                     /locus_tag="ACHJ1G_000490"
     CDS             complement(28611..30272)
                     /gene="yidE"
                     /locus_tag="ACHJ1G_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4762399.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(30469..30897)
                     /gene="ibpB"
                     /locus_tag="ACHJ1G_000491"
     CDS             complement(30469..30897)
                     /gene="ibpB"
                     /locus_tag="ACHJ1G_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4762400.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(31009..31422)
                     /gene="ibpA"
                     /locus_tag="ACHJ1G_000492"
     CDS             complement(31009..31422)
                     /gene="ibpA"
                     /locus_tag="ACHJ1G_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4762401.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(31416..31488)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            31728..32060
                     /gene="yidQ"
                     /locus_tag="ACHJ1G_000493"
     CDS             31728..32060
                     /gene="yidQ"
                     /locus_tag="ACHJ1G_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4762402.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(32062..33276)
                     /gene="yidR"
                     /locus_tag="ACHJ1G_000494"
     CDS             complement(32062..33276)
                     /gene="yidR"
                     /locus_tag="ACHJ1G_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4762403.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            33377..34441
                     /gene="cbrA"
                     /locus_tag="ACHJ1G_000495"
     CDS             33377..34441
                     /gene="cbrA"
                     /locus_tag="ACHJ1G_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4762404.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            complement(34438..35775)
                     /gene="dgoT"
                     /locus_tag="ACHJ1G_000496"
     CDS             complement(34438..35775)
                     /gene="dgoT"
                     /locus_tag="ACHJ1G_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4762405.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(35850..36998)
                     /gene="dgoD"
                     /locus_tag="ACHJ1G_000497"
     CDS             complement(35850..36998)
                     /gene="dgoD"
                     /locus_tag="ACHJ1G_000497"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO4762406.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(36995..37612)
                     /gene="dgoA"
                     /locus_tag="ACHJ1G_000498"
     CDS             complement(36995..37612)
                     /gene="dgoA"
                     /locus_tag="ACHJ1G_000498"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO4762407.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(37596..38474)
                     /gene="dgoK"
                     /locus_tag="ACHJ1G_000499"
     CDS             complement(37596..38474)
                     /gene="dgoK"
                     /locus_tag="ACHJ1G_000499"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO4762408.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(38471..39160)
                     /gene="dgoR"
                     /locus_tag="ACHJ1G_000500"
     CDS             complement(38471..39160)
                     /gene="dgoR"
                     /locus_tag="ACHJ1G_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO4762409.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            39438..40094
                     /gene="yidX"
                     /locus_tag="ACHJ1G_000501"
     CDS             39438..40094
                     /gene="yidX"
                     /locus_tag="ACHJ1G_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4762410.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(40140..40952)
                     /gene="yidA"
                     /locus_tag="ACHJ1G_000502"
     CDS             complement(40140..40952)
                     /gene="yidA"
                     /locus_tag="ACHJ1G_000502"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4762411.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(41067..41465)
                     /gene="yidB"
                     /locus_tag="ACHJ1G_000503"
     CDS             complement(41067..41465)
                     /gene="yidB"
                     /locus_tag="ACHJ1G_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4762412.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(41705..44119)
                     /gene="gyrB"
                     /locus_tag="ACHJ1G_000504"
     CDS             complement(41705..44119)
                     /gene="gyrB"
                     /locus_tag="ACHJ1G_000504"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4762413.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(44148..45221)
                     /gene="recF"
                     /locus_tag="ACHJ1G_000505"
     CDS             complement(44148..45221)
                     /gene="recF"
                     /locus_tag="ACHJ1G_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4762414.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(45221..46321)
                     /gene="dnaN"
                     /locus_tag="ACHJ1G_000506"
     CDS             complement(45221..46321)
                     /gene="dnaN"
                     /locus_tag="ACHJ1G_000506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4762415.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(46326..47729)
                     /gene="dnaA"
                     /locus_tag="ACHJ1G_000507"
     CDS             complement(46326..47729)
                     /gene="dnaA"
                     /locus_tag="ACHJ1G_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4762416.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            48026..48106
                     /locus_tag="ACHJ1G_000508"
     CDS             48026..48106
                     /locus_tag="ACHJ1G_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762417.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            48336..48476
                     /gene="rpmH"
                     /locus_tag="ACHJ1G_000509"
     CDS             48336..48476
                     /gene="rpmH"
                     /locus_tag="ACHJ1G_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4762418.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            48493..48852
                     /gene="rnpA"
                     /locus_tag="ACHJ1G_000510"
     CDS             48493..48852
                     /gene="rnpA"
                     /locus_tag="ACHJ1G_000510"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4762419.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            48816..49073
                     /gene="yidD"
                     /locus_tag="ACHJ1G_000511"
     CDS             48816..49073
                     /gene="yidD"
                     /locus_tag="ACHJ1G_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4762420.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            49076..50722
                     /gene="yidC"
                     /locus_tag="ACHJ1G_000512"
     CDS             49076..50722
                     /gene="yidC"
                     /locus_tag="ACHJ1G_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4762421.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            50828..52192
                     /gene="mnmE"
                     /locus_tag="ACHJ1G_000513"
     CDS             50828..52192
                     /gene="mnmE"
                     /locus_tag="ACHJ1G_000513"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4762422.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            52435..52509
                     /gene="tnaC"
                     /locus_tag="ACHJ1G_000514"
     CDS             52435..52509
                     /gene="tnaC"
                     /locus_tag="ACHJ1G_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4762423.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            52730..54145
                     /gene="tnaA"
                     /locus_tag="ACHJ1G_000515"
     CDS             52730..54145
                     /gene="tnaA"
                     /locus_tag="ACHJ1G_000515"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4762424.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            54236..55483
                     /gene="tnaB"
                     /locus_tag="ACHJ1G_000516"
     CDS             54236..55483
                     /gene="tnaB"
                     /locus_tag="ACHJ1G_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4762425.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            55615..56790
                     /gene="mdtL"
                     /locus_tag="ACHJ1G_000517"
     CDS             55615..56790
                     /gene="mdtL"
                     /locus_tag="ACHJ1G_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4762426.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            56765..57724
                     /gene="yidZ"
                     /locus_tag="ACHJ1G_000518"
     CDS             56765..57724
                     /gene="yidZ"
                     /locus_tag="ACHJ1G_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4762427.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            57881..58630
                     /gene="yieE"
                     /locus_tag="ACHJ1G_000519"
     CDS             57881..58630
                     /gene="yieE"
                     /locus_tag="ACHJ1G_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4762428.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            58652..59218
                     /gene="yieF"
                     /locus_tag="ACHJ1G_000520"
                     /gene_synonym="chrR"
     CDS             58652..59218
                     /gene="yieF"
                     /locus_tag="ACHJ1G_000520"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4762429.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(59272..60609)
                     /gene="adeP"
                     /locus_tag="ACHJ1G_000521"
     CDS             complement(59272..60609)
                     /gene="adeP"
                     /locus_tag="ACHJ1G_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4762430.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            60776..61441
                     /gene="yieH"
                     /locus_tag="ACHJ1G_000522"
     CDS             60776..61441
                     /gene="yieH"
                     /locus_tag="ACHJ1G_000522"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4762431.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            61508..61981
                     /locus_tag="ACHJ1G_000523"
     CDS             61508..61981
                     /locus_tag="ACHJ1G_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762432.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            62030..62617
                     /gene="cbrC"
                     /locus_tag="ACHJ1G_000524"
     CDS             62030..62617
                     /gene="cbrC"
                     /locus_tag="ACHJ1G_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO4762433.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(62679..63401)
                     /gene="yieK"
                     /locus_tag="ACHJ1G_000525"
     CDS             complement(62679..63401)
                     /gene="yieK"
                     /locus_tag="ACHJ1G_000525"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4762434.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(63416..64585)
                     /gene="yieL"
                     /locus_tag="ACHJ1G_000526"
     CDS             complement(63416..64585)
                     /gene="yieL"
                     /locus_tag="ACHJ1G_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4762435.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(64612..66228)
                     /gene="bglH"
                     /locus_tag="ACHJ1G_000527"
     CDS             complement(64612..66228)
                     /gene="bglH"
                     /locus_tag="ACHJ1G_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO4762436.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(66314..67708)
                     /gene="bglB"
                     /locus_tag="ACHJ1G_000528"
     CDS             complement(66314..67708)
                     /gene="bglB"
                     /locus_tag="ACHJ1G_000528"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO4762437.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(67727..69604)
                     /gene="bglF"
                     /locus_tag="ACHJ1G_000529"
     CDS             complement(67727..69604)
                     /gene="bglF"
                     /locus_tag="ACHJ1G_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO4762438.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(69738..70574)
                     /gene="bglG"
                     /locus_tag="ACHJ1G_000530"
     CDS             complement(69738..70574)
                     /gene="bglG"
                     /locus_tag="ACHJ1G_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO4762439.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        17526 a        18621 c        17484 g        17109 t
ORIGIN      
        1 tataaacgat ttgaatcaag tggttatatg gcaattttga ctcctgtgat cttccgccaa
       61 aatgcctctt ctgacgttct ccaacccctt gtaatactgt caatccagat attttctact
      121 gctcaatatt caatcggctt caggaatacc tggtctaagg ttttgtgatg gacgatgagc
      181 ggctgaagaa tccgcctgtc ggttcttctg tcctcgaacg catccgcgat atgcgcgcca
      241 gcatgtgccg tgtttatttg caggtgattt gccccctgct ggctctcagc caactgtatc
      301 gccattaagg tgaagatgtt gttttactcg ccccagaaca ccctttttta tacgactatt
      361 ctatttcttt aaccgctggc gaaaaaaact gactataaac ttcaccaata atcagactgc
      421 catcaagatt aatttgttca tttaatatat caaatcaact tgttttattt ttactatcaa
      481 aaatagctca ttgccttact ataattaata tataaattcg gtaattaatt cttaacatgc
      541 ttttgctaat aatctcaatt gctggcccta taatattgcg ctagcattgc tttctggttg
      601 tatcagcgat actcaaaaat tcttaataca atactcattc gactggtact tatttgtaac
      661 tcagttatat tttgtcgccc ggtgattcag aagaatgcaa aaaacggcta ccactccatc
      721 aaaaatactt gatctcactg ccgcggcatt tttacttgtc gcctttctga cgggtattgc
      781 gggcgctctt cagactccta ccctaagtat attcctcgga gatgaactga aagcccgtcc
      841 tataatggta gattttttct tcaccggtag cgctattatg ggaattctgg tcagtcaatt
      901 tctggcaagg cactccgata aacaaggcga ccgtaaatta ctgattctgc tatgttgctt
      961 atttggagtg ctggcctgca cgctttttgc gtggaatcgc aactacttca ttctcctctc
     1021 aacgggcgta cttctgagta gttttgcttc caccgcaaac ccgcaaatgt tcgccctcgc
     1081 ccgtgaacac gccgacagaa caggccgtga gacggtcatg ttcagtacat ttttacgtgc
     1141 tcagatctcg cttgcctggg ttatcgggcc accgctcgct tatgaactgg caatgggatt
     1201 tagttttaaa gtgatgtatc tcaccgctgc catcgcattt gttgtttgcg gactgatagt
     1261 ctggttgttt ttgccatcaa tacaaagaaa tattcctgtc gttacccaac ccgtagaaat
     1321 tttaccctcc acccacagga agcgggatac gcggctactt tttgtggtct gttcaatgat
     1381 gtgggcggcg aataatctct acatgataaa tatgccgcta tttattattg atgaactgca
     1441 tctaaccgat aaactggctg gagaaatgat ttgtatcgct gccggtctgg aaattccgat
     1501 gatgttaatc gcaggctatt acatgaaacg tattggcaag cgactattaa tgctcattgc
     1561 tatcgtgagt ggaatgtgtt tttacgccag cgtactcatg gcgacgactc cggcggttga
     1621 gctggaattg caaattctta atgccatctt ccttggtatt ctctgtggta tcggcatgct
     1681 ttattttcag gacttgatgc ctgaaaaaat aggctctgcg acaacgttat atgcaaatac
     1741 ttcacgcgtc ggctggatta tcgccggctc tgttgacgga attatggttg aaatctggag
     1801 ctaccatgcg ttgttctggc tggcgatagg gatgttgggt attgcgatga tttgcctgct
     1861 gtttattaaa gatatttagc tcaccagtct gggctaagaa aaatcggcgt ggcgcaaaca
     1921 gagccatcct cgccattttt gctatcatgc ctgcatacat aaacgacaaa acagtatgca
     1981 gagggaaaaa tgggttccac cagaaagggg atgctgaacg ttctgattgc cgccgtgttg
     2041 tggggaagtt caggggtctg tgcgcaatac atcatggagc aaagccagat gtcgtcgcag
     2101 tttttgacta tgacgcgttt gatattcgcc ggtttgattc tactgacgct gtcatttgtt
     2161 catggcgata aaatcttttc tattattaac aatcataaag atgccattag cctgctgatt
     2221 ttttccgtgg ttggcgcgct aactgtacag ctcacttttt tgctaaccat cgaaaaatcg
     2281 aacgcagcca cggcaacggt gctgcaattc ctctcaccga cgattatcgt cgcctggttc
     2341 tcactggtgc gtaaatcgcg cccgggcatt ctggttttct gcgctatttt gacatcgctg
     2401 gtcgggactt ttttattggt gacacacggt aatccgacgt cattatcgat ctctcctgcc
     2461 gcgttgttct ggggcattgc ctcggcattt gctgctgcat tctataccac ctatccctca
     2521 acactaattg cccgctatgg cacgttacca gtcgtcggct ggagtatgct gattggcggt
     2581 ctgattctgt tgccttttta tgccagacaa ggaacaaact ttgtcgttaa cggcagtttg
     2641 attctggcgt ttttttattt gatcgtcatt ggtacgtccc tgacatttag tctgtacctg
     2701 aaaggagcac aattaattgg cggtccaaaa gccagcattt tgagctgtgc agaaccatta
     2761 agtagcgcgc tactctcttt gctgttgctg gggatcacgt tcacattacc ggactggctg
     2821 ggaacgctgc taattctgtc atcggtaatt ttgatttcaa tggattcccg tcgccgcgcc
     2881 agaaaaataa atcgtccggc gcggcatgag tgagaattac cagccaggca ccgcgccacc
     2941 gttaaaaatg gtttctgccg ctttagcgac ttcgggtgat tgataagatt gcagaaattc
     3001 cttcacgttt tctgcattct tattatcttc ccgtgccacc aaaatattca catacggcga
     3061 attcttatct tcaataaata cgctgtcgtg caccggagaa agccctgtct gctgaatgta
     3121 agtggtgctg ataatcgcta catcaacttt gggatcatcc agtacgcgcg ggagctgcgc
     3181 cccctcgagt tccataatct gcaaatgacg aggattatcg gttatatcca gcgcggtagg
     3241 taataaccct tttccctctt tcagagtaat tagcttctct ttttgcaaca gtaaaagtgc
     3301 gcgaccaagg ttggtcgggt cattggggat cgccactgtc gcgccttctt ttatttgcgc
     3361 cacggttttg atttttttgg aataacccgc catagggaag acaaacgtat ttcccaccgc
     3421 taccagttta taaccatgcg cctgattatc ttgttcaaga aagggtcgat gctggaagac
     3481 attcgcatca agttcgccat gatttgtcgc atcgttcggt agcaacgaac cgctaaatcc
     3541 taccagttca acatcgaggc catatttctc ttttgccacc tttttagcga cttccgcgac
     3601 atcttgttct gcgccattta taacgccaac tttaatgtgt tttgcatcgc tgctactctg
     3661 gtcgcaacct gccagcagaa ttccggccag caataatgcg gcccctgtcc gtagatgatg
     3721 tgttgtcagt ttcatttttt tatcctttta ttaatgatta cctgactata gcggtaaggt
     3781 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
     3841 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
     3901 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
     3961 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
     4021 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
     4081 gtaaaatttg cacgccacag gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
     4141 cagatgaaaa caataaacaa catatacacg aggccatcgt ggcgatggaa cgtaataatc
     4201 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
     4261 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
     4321 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
     4381 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
     4441 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
     4501 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
     4561 aacgatttta tcgcttcccc acaacgtcag caccaacgca ctcacagcca gtattaacgg
     4621 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aatgacgaaa gcgacgtacc
     4681 gataaagctg gcaatgccaa aactcaacag caccagcgtt aagccatcca cgctgaatcc
     4741 cgccaggttc atatacaccg ggcgaatata tgtgaagaaa gcaaactgcc cggcgaaaga
     4801 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     4861 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     4921 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     4981 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     5041 cgacagcgcc ttcggcaccg tacgcggcgg caccagacgc atggtcagcg acgccgacat
     5101 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     5161 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     5221 aacgtagcgg cggtcagtag cctgaattgt ctgggtaata aacaaactgg caaacattgc
     5281 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     5341 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     5401 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     5461 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     5521 ctttaacatt gaaatgtgtg acgcatttaa cgtttttgca acttttacgg tgttgcttga
     5581 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     5641 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     5701 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     5761 atcctgcatt tcaggttcgg tttcgcgcag aaaacctagc gccagcctga cctgagcgtc
     5821 ggtaatcggc gcggcgaggc gagctttttg taaaatccga tgccattgca gcaggttctg
     5881 ttcgctacca tcgacaatga caatttgcca gatgagcaac acctgcgcgg cgttttgcag
     5941 gtgcgactca tccggacgat gcaaataacg taaaaattcg tcaccagctg actttcccat
     6001 ttgatcgacc atcgactcca cgggtacgac atcgatgttc agccgatcgc tgagacggcg
     6061 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     6121 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     6181 aaaccctcgc cagttactaa tccaccagaa taatcttcag tgcaaacaga gctgcgacca
     6241 ccaccacaca caggttcaga tcgcgccagc gcccggtgca tactttcatg atgcagtacg
     6301 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     6361 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     6421 tcatcagcac gccaacaaag attaacgctc cggcggttgc gtaaggagga accatcgcca
     6481 ccagcggtga gaagaacata actaacagga acataacgcc aaccacaacc gcagtcagcc
     6541 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     6601 tgccgataaa cgcccccgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     6661 tggggaattt accgttacta tctattaagc ccgctttatc agttacacca attaatgttc
     6721 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     6781 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     6841 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     6901 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     6961 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     7021 tggcgacaat aacgccagta ttttttaatc ccattaaggc gataaataat ccaattccgc
     7081 tggtgatacc aatacgtaaa cttaagggaa tgttggagat catccagtac cggatacgaa
     7141 acagcgtgag caaaaatagt ccaactgcgc cccagaatat tgcgcccatc ccggtctgcc
     7201 aggagatgcc catcgccccc acgaccacga aggcaaagaa ggcgttcagc cccattgccg
     7261 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     7321 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     7381 ggttcacaaa aacgatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     7441 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     7501 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     7561 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     7621 acattaatgg atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     7681 ttgataaaat caaaatgaga aaaatatgaa taattctatt aaccataaat ttcatcacat
     7741 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     7801 tattattgat aatgtctcta ttctcgacct gatcaatggc ggagaaattt ccggcccaat
     7861 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgatg ctccggcttt
     7921 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     7981 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     8041 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     8101 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     8161 cgtacccgcc ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     8221 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     8281 aattagcggg cagaatgcgc tgctcgataa actggatgca tttcgccacc tgacgctgga
     8341 cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc tatattactg cgggtattga
     8401 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     8461 gttgatgatc cgcgaagggt ccgctgcccg caatctcaac gcgctggcac cgttgatcaa
     8521 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     8581 ccatgaagga cacatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     8641 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
     8701 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     8761 cacggtgcag caggtactgg tgaaaggcga gccgattgat gcgcaaacct tacaggcgga
     8821 agagtcggcg agactggcac aatccgctcc gccatatggc aacaccattg cccgccagcc
     8881 agtttccgcc agcgactttg ccctgcaatt tacgcccgga aaacgctatc gggtcattga
     8941 cgtcatccat aacgaattga ttacgcactc ccactccagc gtctacagcg aaaatggttt
     9001 tgatcgcgat gatgtgagct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     9061 ggcttgtggt ttgcttggcg gctttggact gaatgaaggt gcgctggctg cgacggtcag
     9121 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     9181 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aggtccaaag
     9241 tcatctgccg ttgcccattg ccggcctgat gagcaccgac acggcgcagt cgctggcgga
     9301 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     9361 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     9421 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaag
     9481 cccggcgtca tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat
     9541 ttttttctcg cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat
     9601 acaaccaatc gcggcgatat caagtgcggc gaaggtgcct gcccagccgg taaggccgaa
     9661 taccggcgtc ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag
     9721 gtaagcaaag gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc
     9781 aacagcagcc acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag
     9841 agaagccaga tagatatatt cgttgctggc atgttgatac acaccgagcg tggcgataat
     9901 cagcgccagc gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca
     9961 gccccacagc agcgtaccga ccagtgcacc agcttcaaac agcgtaaagc cctgaatcgc
    10021 caccgcttta gagagtttca gttcctggaa cgcgtatacg gttgaccact ggtcgatacc
    10081 aatacgtacc acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt
    10141 cagcacatac tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc
    10201 ctcttcgctg atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg
    10261 ggagtcgctg ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa
    10321 gataaacatg ccgatgacat ggccatcgaa caggtaattt gccccgaaca gcgccacacc
    10381 tgctgcgcct gcaccgccaa ggttgtgaga aatattccag aaaccgagga atgtcccgcg
    10441 tttacgacgc ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtactctg
    10501 gaaaaagccg cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc
    10561 catactggca ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa
    10621 ttgtttggtg tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt
    10681 gatggagaaa cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgagat
    10741 catgtcgttc tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat
    10801 aaagaccacc aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag
    10861 cggaaggtcc agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg
    10921 atttcccttc atacttcaag ccgcaggtgc cttagctgca cgcattcctc ccggtcacat
    10981 agttcactat gctcccggga ctcacgcgtt tgctgcgttc ctgcaacttg aattattttg
    11041 ggaaagaatt aatgaatgat ggcattgtaa aaatcagcag caaatcaggc ctgagatagc
    11101 gtccaggcaa aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat
    11161 gcatcacgct tcgcgcggtg cctgggcgtt caaaaagggc agtaacagca gtgcggaaac
    11221 cccggcggcg atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt
    11281 cgccagcggc caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt
    11341 cgccgcccct gcagcctctt tgtgggaaca ctctgccgcc gccatgccga ttaacatctg
    11401 cgggccaaag acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc
    11461 aaatggcatc agccacagcg agccgactga aagcaaaatt ccggcggcga aaatcaaatt
    11521 catcggccca cggttgccgt taaacaattt gtccgagccc caaccggcta ccagcgcacc
    11581 gataaatccg cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc
    11641 cagcgtctcg gacatataca aattgcccca gtcgttgatt gccgcccgga ccacatagac
    11701 cagcacatag caaaacgaaa gcagccagat atacggattc agcaacacat atttggtcag
    11761 gatctcttta cgcgtcaacc ctgccccttc ttgttgttga gcaatttcca gcgcgtcgtg
    11821 ccgccattca ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgta gccgccagca
    11881 gagaaaaatc cccacgacta tcgccataca accagcaatc atcatcccgg cacgccagcc
    11941 gtaatgcagc gcgctcgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc
    12001 cgtgttccat aatgcccacc aaccgccgcg ctcggtacgt gaataccagg ccgttaacag
    12061 acgcgcacac accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagcacggc
    12121 aaacgcccat agcgacgtcg agaagccaaa cagaatgttc atgatgcccg tggcgataag
    12181 ccctatcccc ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt
    12241 cgacacgccg taagtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag
    12301 cacgccgtta gcaaggattt ctggtaccgc ggcgttaaaa cttttccgcg tgaagtaaaa
    12361 cagcgcgtaa cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc
    12421 atcaatttca tatttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa
    12481 catcatcaaa ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag
    12541 acaggaaatg tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc
    12601 aaaaccttgt tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat
    12661 caaccgttca tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac
    12721 aatgttgttc agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa
    12781 cgctgattca tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat
    12841 ttcccgcatc agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg
    12901 taaccgaccc aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag
    12961 ctgcccgctc tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg
    13021 agtacgaatg gctgtgatgg tctgaccgat atcatcatgc agctcacgcg ccacatcacg
    13081 gcgcacgctc tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc
    13141 cttttgcagc gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc
    13201 tgtcagactt tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg
    13261 actggcgatc agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata
    13321 gtgccaggcc agcgcgataa tcggcagcgc cagacaaaat ggcgtaaagc gcgacagttc
    13381 gtccggcaat cccaactgga gccagagact gataacaaac agcagcaagt accagaccag
    13441 atgtcgcccg cgccagttga tcggctgaga aaccagtgac gggccgagcg gcagccaggt
    13501 gttattggcg agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt
    13561 taaagtcagc aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg
    13621 caacaacgcc gccgccgtta acgccgcacc ctgtaacagc aaggtgcgcc agtcacgctg
    13681 atggcgatag cgcgagatca gcgccacggg cagtaacgtc agtaaactac cgatcatcaa
    13741 taacggaaaa tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc
    13801 gcccagcagc accggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc
    13861 aaacggaaat aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca
    13921 caggcaaaac catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga
    13981 gaacaacgtc ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg
    14041 acacccagtt tttccatcag gttggcgcga tggacgtgca ccgttttcgg cgacaagccc
    14101 agttcggcag caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctgccgt
    14161 tcgcgtttgg ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc
    14221 agataacagc cgcccgtggc aaccgtatgc accgccgcaa tcagttcgtc agggctacag
    14281 cgtttggaga gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgccggactg
    14341 tcatgaacag agagcattat tgtcgccata cctttcggca gctggcttag cagttccaga
    14401 ccggagatat cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc
    14461 cccgccagcg cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc
    14521 cccagcagct gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg
    14581 atcatggtct tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg
    14641 ttgttcaagc cttgagcggt tactgaaaaa acaccgcaat cttgttaaac atagtcggat
    14701 cggactgatt acgctgcact ttcaccacat cttccagctt atcgatttgg cttatcatct
    14761 gctccagacg ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa
    14821 tcggcagaca aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt
    14881 gggtcattac gcccggatgg ttgcgaacgg tgagctccag aatcacgttg tcatgagttg
    14941 tgttttgcat ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg
    15001 ggtaaacttt ttcttcggca tcaatgcgca catggatcag cgccgggcca gggcgattga
    15061 tgatttcctg caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc
    15121 cgaatccggc ggcaatctgc ataaagttga tttttcccgg ataggtggcg gcaaaaacgc
    15181 cttgctcgta gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca
    15241 gaatgatttt gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca
    15301 tcaggctgcc gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc
    15361 caatcgccgc aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc
    15421 gtgggcgatt gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg
    15481 tgataatcgc attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta
    15541 atggatcgca cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact
    15601 ggtgccactc tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat
    15661 caacatccgc ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga
    15721 tatcgacatg aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt
    15781 catcaaaacg cgcaccgagc actatcaaca aatccgcctc ctgcaaaata tagttggtgc
    15841 tgcgcacgcc gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcaacatgc
    15901 ccagcgccat taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc
    15961 gtgcgggcgc attgatcaca ccgccgccca gataaagcac cgggcgtttg gcagcgttaa
    16021 tcatcgccgc tgcgtcacga atgctttctt cgctaaaggc gggggcggcg gctttttctg
    16081 ccatagcggg ctgtgtttca atctcaaaaa ctgccgtttg cacatcctta ggaatgtcta
    16141 tccacaccgg gcctgggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg
    16201 ggagttcttc gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt
    16261 aggtgtccac ttcctggaag acgtcggtgc cgatcatcga ggcgggaacc tgaccggtga
    16321 tgcaaatcag cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg
    16381 cacccggtcc gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccatcccct
    16441 gagcgataaa tcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt
    16501 ggcttaaggc atcgtaaaca ggcaggatag aaccgcccgg aatgcccgtc acaatcttaa
    16561 tgccctgctg ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtcg
    16621 atgttgtgcc cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg
    16681 tgcttaaacg aaaaaccccg cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt
    16741 ccagtcccta cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg
    16801 atagcgcagt tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg
    16861 gttttgtgtt taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt
    16921 ttcagcaagg gaaaattgag gggctgatca cgttttgtac tgaattgcag gtaacaaaaa
    16981 accccgccgg agcgaggttt catcagccgc ctgcggctgg taaccgcaaa gcacactgta
    17041 ttatgtcaac actgaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt
    17101 tgacgtattt agggcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattact
    17161 gtttatttat acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg
    17221 catcgttacc cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc
    17281 ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt tctccttaag ataccgcgtg
    17341 cacaacagct ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca
    17401 ggagacgcgt atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc
    17461 actgcaactg cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca
    17521 aaggggagcg ggtaaccgct ccccttttat atttagcgtg cgggttggag ttgggggctt
    17581 cttctaccag cagaatgttt gcacctgcgc tgcctgtgcc cctcacccta gccctctccc
    17641 caaaggggcg aggggaccgt cccgtgcaat gtttagctca ttttctgaaa gggaacggtg
    17701 gtttccccct cgcccctttg gggagagggt tacagttcgc acgtaatccc tcccggcaac
    17761 atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt cctggcgtat
    17821 atctggctaa cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt
    17881 gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcaa agaaacgtca
    17941 atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata
    18001 ttccagctat tgccgatatg gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg
    18061 taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc ccgatttccg
    18121 accgcgtggg ccgccgaccg gtgatcctcg tcggaatgtc catttttatg ctggcaacgc
    18181 tggtcgcggt cacgacctcc agtttgacgg tgttgattgc cgccagcgcg atgcagggga
    18241 tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgagattta tatgaacgga
    18301 cacagttgcg ccatgctaac agcctgttaa acatggggat tctcgtcagt ccgttgctcg
    18361 caccgctaat cggcggtctg ctggatacga tgtggaactg gcgcgcctgt tatctctttt
    18421 tgttggttct ttgtgctggt gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc
    18481 cggtcgatgc accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg
    18541 gttttaactg ttatttgctg atgctgattg gcggtctggc cgggattgcc gcctttgaag
    18601 cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt
    18661 tgtttattct gccgattccg gcagcgtttt ttggcgcatg gtttgccgga cgtcccaata
    18721 aacgcttctc cacgttaatg tggcagtcgg ttatctgctg cctgctggct ggcttgctga
    18781 tgtggatccc cgactggttt ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc
    18841 tgttcttttt cggtgccggg atgctgtttc cgctggcgac cagcggcgcg atggagccgt
    18901 tccccttcct ggcgggcacg gctggcgcgc tggtcggcgg tctgcaaaac attggttccg
    18961 gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctggggttgt
    19021 tgatgacctt aatgggattg ttgatcgtgc tgtgctggct gccgctggcg acgcggatgt
    19081 cgcatcaggg gcagcccgtt taagcgcacg tcaccgcagc atcgtcatca gctccatggg
    19141 agaacgatgc tgctttatca gatcacgcat cacccgcata tgcggtgcgg agtaagaata
    19201 aaacgcctga tagcccgcac aaagcacgct gtctggcgtc tcctggcgag cgtgcgggca
    19261 caggcgatgc cagtcgcacg cctggcagtc acggcgcggt tgcgggtttt cgctgccaca
    19321 ccatttttgt aaggtggcgt caaaaagctc aatcgagata cgactgatat ctgcccgcac
    19381 ccaacactcg aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc
    19441 cgtcagatcg ccccggtcat caaattcaag atagggaatg agctgtacgg agcggatgcc
    19501 ccgctgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttgt actctacaac
    19561 cagtttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca
    19621 ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc
    19681 gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg
    19741 acatccatta aattgcgatt acgggtgtag tgccagagga tcagcgccac gatggcgaga
    19801 atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac
    19861 gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt gcgctccggc
    19921 tgcagtcctg ggtcggcaat gcgtctggct ttgcggctat ccggcataca acaccagtcc
    19981 cataacgatc accgccacga ccatcaatat taagctaatg attaataagc tgttggtgta
    20041 gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca ggtagccata
    20101 catcgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcac gaatgacggg
    20161 cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc
    20221 ggtacggatc cacgccagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc
    20281 ttctccaagg cgggaaattt tcattgttgc tcctttgcaa tttcactaac gctctacctt
    20341 tagcggtttt tttttaagaa tttcgtccgc ccgtttcctg atatgctttc gccatccgct
    20401 acaaggaggt cagatgggta tcatagctca aaataaaatc agttcattgg gcatgctgtt
    20461 cggtgccatc gctttaatga tggggattat ccacttttca ttcggccctt tttccgcacc
    20521 accgccaacg tttgaaagca tcgtggcgga caaaacggct gaaattaaaa gaggtcttct
    20581 cgccggaata aaaggcgaaa aaattaccac cgtggagaaa aaagaggacg tagatgtcga
    20641 taaaatcctc gatcaaagcg gtatcgcact ggccattgct gcgctacttt gcgcatttat
    20701 tggcggtatg cgtaaggaaa atcgctgggg cattcgcggc gcgctggttt ttggcggcgg
    20761 gacgctggct ttccatacct tgctttttgg tatcggcatc gtgtgcagca ttttgctgat
    20821 attcctgatt ttttccttcc tgaccggcgg ttcactggtt taagcagcct gaataatgcc
    20881 cgccgggtgt tcatcagcgg gcattatcgt cagaacttct gttttttctc ttccactttc
    20941 actccttgcg tcggtaggcc ggtgtcatag tcacgtacca ccggcgaata gccatcttgt
    21001 gggcgtggac gaaatgcccc catccagcgc ggcagtgcat ctttacgcca cggacgcagg
    21061 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    21121 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    21181 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagtac cagtttaaag
    21241 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    21301 tagcggttaa attccaccat tacaccgcgt ggctctttca cggcaaggat attttccccc
    21361 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcgata
    21421 tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga
    21481 atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc
    21541 atttcgccgt gatcggaagt ataaatgacc cacgtatttt cacgttgctc tggcgttaag
    21601 gcattgatga cccgtccgat ttggtcatca acaaagtcat tacaggcaaa atagagcgga
    21661 tggtgataaa gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga
    21721 tgttccggtt tgttcgccag gccatcctga gctttctcgc ccagatcgta gtaaaaatca
    21781 gcgtatttct ctaaatactc caccggacag gtgaacgggt gatgcggctc atcatacgaa
    21841 accaccatca ggaagggttc ctcggcgcgc gcgggctgtt gcagaaaatc tactgcccga
    21901 ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcgatat ggttcgcctg taaatcctca
    21961 acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaaggtag
    22021 ttcgccccat cgaaccagta atcagcgtcc cactccggcg gacactcgcc agtgccgaaa
    22081 tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta
    22141 aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg
    22201 gactggttag cgtagatacc ggtaaatagt ccggcgcgtg caggcgtaca aaccggtgaa
    22261 caggtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta
    22321 ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcatgatg
    22381 aacagaaaat tggggcgttt cataggctat ccttcgcgtc gggagtaaag gttacggctg
    22441 ggtcgtgacg atgccgccag ctgtcaaaaa tcaggtagac aatcactacg gcggcaatga
    22501 aataactgat catcgctaac cagcgcgtac cgtagccgcc gaattcagcc agcccggcat
    22561 agacgccaat catcgcgaac aggatgccaa ttgacgcgat cttgacgttt ctccacggtt
    22621 tcatgtccac cgcaaacgca tctttgaagg tgaacggcgt ggcgcgcggt ttgataaaac
    22681 cgatcaccag catcacgacc acgttgatgc agaacgtaca ggccagcaca tagaggaaat
    22741 ggaagtcgaa cttcaccaga tagttgatgg tgatgtagct gattatgcca atccccatcg
    22801 ccacttttgc cgccagcgcc gggatgcgcg ggaagaaaaa gcccataatg atgatggtaa
    22861 ccagcggcac gttgtaaatg ccgttgagct gtttcatcca gctatacagc ccctgcggcg
    22921 cgttggcgat ccacggcgcg accagcaccg aaacgatagc gataaagaaa ccaaatttgc
    22981 gcccgacggt gaccagctgc tgcggctcgg cattctggtt aatgatgcga cggtaaatac
    23041 ccatactgaa taacgtactg gcgctattca gaaagccgtt gaaggtactg atcaccgcac
    23101 caaataacac cgcgccgaag aaacccacca ttggcactgg cagaacgtta ttgaccagcg
    23161 tcgggtaggc catgtcggct ttcggtaaat cctgatacag atgaaacgca atcaaccctg
    23221 gcagcaccag taccagcggg tcgagcattt tcagcaccgc cgttaacagc gccccctttt
    23281 gcccttccgc caggcttttt gacgccagcg tgcgttgcac gatgccctga tttgtacacc
    23341 agtaaaaggt gttcaccaga atcaaaccgg taaatgccgc gccaatcggc aagggatcgg
    23401 tcgggccacc gattgagttt aatttctcgg cgtgaacggt ggtgagttgc tcaatgccct
    23461 gcataaagct gcccttgccc atcgcgatca ggccaaatac cggcaccatc aacccgccga
    23521 taaccagccc aataccgttg atggagtccg ccactgccat tgcgcgcagt ccgccgatca
    23581 ccgcatacaa aattcccgcc agaccaagca aaattaccag tagccagata gccgcaccgt
    23641 gagaaatctg tagcgattcc ccgacgtgaa acaggctgtt gagcgccaac gcgccggagt
    23701 agagaacaat cggcagaaag cagacgccgg tggcaatgag gaagcagaag tcgatgataa
    23761 tacgcgtcgt tttatcataa cgttcctcca gaaaatcggg gatggtggca atgccgcgct
    23821 tcagatagcg cggtaaaaag attagcgcga ggaagatcag cgtcaccgct gaagtcactt
    23881 cccagcccat caccgacatg ccgcttttgt aggcctgccc ggaaaggccg accagttgtt
    23941 ccgtggaaag gttggttagc attaacgaag cggcaataac cggcgctttt agtgaacgtc
    24001 cggcaagaaa atagccttgt tgcgatccgg tatctgtttt gcggaccttc caccaggtga
    24061 tcaccgccac cagcagcgta aaaccgacaa aactcaagat ttgtaacgaa ttcatctcat
    24121 agcccttatt tatgtttatg tcccggacgc atgaatatag ggagaggtaa aatgcgtcgg
    24181 gtcaggcagt cagattctta aaatacaaac gtcgtatccc tgaacggatt gttttctgtt
    24241 aagttcaggt tgtgagcata attcgccgga gggatattaa aatgaatgga aaattgcaaa
    24301 gttcagatgt aaaaaacgaa actccgtaca atattccctt attgattaac gaaaatgtga
    24361 tctccagcgg aatttctctg atctcgctct ggcataccta cgccgacgag cattaccggg
    24421 tgatctggcc gcgggacaag aagaaaccgc ttatcgccaa ctcatgggtt gcggtgtata
    24481 ccgtacaagg atgtgggaaa attcttttaa agaatggcga acaaataaca ctgcatggca
    24541 actgtattat attcttaaag ccaatggata ttcactctta tcactgtgaa ggtttagtct
    24601 gggaacagta ctggatggaa tttaccccca ccagtatgat ggatattccc gttggtcagc
    24661 aaagcgttat ttataatggc gaaatttata atcaggaact caccgaagtt gctgagttaa
    24721 taacttcacc agaagcaata aaaaataatc tggcagtcgc ttttctgacg aaaattattt
    24781 atcagtggat ttgtcttatg tacgcagatg gtaaaaaaga tccacaacgg cggcaaattg
    24841 aaaaattaat tgccacttta catgccagtc tgcaacaacg ctggagcgta gctgatatgg
    24901 ctgccacgat cccctgtagc gaagcctggt tgcgtcgtct gtttttacgc tataccggca
    24961 agacgccgaa agaatattac ctcgatgcgc gtctggatct ggcgctatcg ctattaaagc
    25021 aacaaggaaa ctcggttggc gaagtcgctg atacgctcaa cttcttcgac tcctttcatt
    25081 tcagcaaagc ctttaaacat aaatttggtt atgcgccgtc agccgtgcta aagaatacgg
    25141 accagcaccc aacggatgcc agtccacaca attaacgtaa ctctggccag tacgctttgt
    25201 tggcctccag caactcatcc agaatagctt tggcgaccga ggcgctcggt accgttttcg
    25261 acaacgtaat cgcctgccac aggtgctgat atgaacgctg ttcccaggcg tccacgacca
    25321 gtttttccac cgccacttgc tgactcatta accctttctg aaactgcggg atatcgccga
    25381 ccaccagtgg ttcaggccgt agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac
    25441 aatttgcgcc atccggcttt caataccgat tggcatgtca cagatgttga ggattttcgc
    25501 attcgggcgc agacgacgcg tggcttctgc gacaattgct gccgggttgg agtagttgag
    25561 catccaggcg tttggtgaat atttttccat ataatccacc agttccagga cgccgccaat
    25621 ggaacgcatg ccgtacgcta ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg
    25681 gcgcagtggg attttttcat ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc
    25741 catgacaaaa tcaacgtcgc tgaacgctac ttcaggatcg gtggtgtaac taaacgcaat
    25801 gtccggcgct ttttctttaa ggatgacttt acaggcttcg gcaatcactt cctgccgcgc
    25861 gccatcgtta tcataaaatt tcagtgcacg aagcgggaaa cggtcctgat tcgccaggag
    25921 catcaacacg atgcctggcg taaaggtgct tccaccgcct gcgacaacca ctgagaattt
    25981 ggtcattata ctgcctccgt aatggcaaca ttttctgctg attgatgaga attaattaag
    26041 ctatcgagct gttcacgcaa ttgggataca tgcagaccaa tgatcacctg aatggcatcg
    26101 ccactacgga agacgccgtg cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt
    26161 gacatgtcat gcagtgcaat gcgtagacgc gtcgcgcaat tgttaatgct ggagatattg
    26221 ccgaccccgc ccagggcttg cagaataccg gcagcctgat ccagctcttt ttttggctct
    26281 gccgcggtgg tttggcctcg cgaggctttg tattcggctt ttgagtagag tttcacttcc
    26341 gcatcttcac gtcccggcgt gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg
    26401 aagtacagca gggtaaagca caacccaatg gcgatttggg tcagcatcat atccgcgtgg
    26461 ttgctgaaca tcgggatcca gttttgcggt aaaacctggt caatcagacc tccgcccatg
    26521 ttgcccacca caccaaagag atacatcacg gtcgacattg aggccgccag cacggcgtgt
    26581 accgcaaaca gcaacggtga aatgaacagg aaggtaaatt ccagcggttc ggtaattccc
    26641 accagcatgg cggttaaggt tgcgggaatc agcaagcccg ccacttttac ccgattttcc
    26701 ggtgcggcag tgaagtacat cgctaaagaa atgcccacgg caccaaagat ttttgagtta
    26761 ccgtgcaggg caaaaccgcc ttccgggaac aacgatttca gcggctcggc gctcagactg
    26821 aactcttgca gatgctgcgc ccagtacatc tgaatgccgc cttcaacagc tgccggacca
    26881 aagataaacg gtccgtagat gaagtggtgt aaaccggttg ggatcagaat acgttcgagg
    26941 aaggtgtaaa cccacacccc aagcgcaccc gccgaacgca ggaacgcttg cagagattca
    27001 atccccattt gtacttttgg ccagccgagc aacgtcagcc aggcacaggg gatcatcacc
    27061 aggaaggcga taatcaccac ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt
    27121 tttttatcga acagacggtt atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg
    27181 ataatgctgg tatcgagggt tttaatcccg gccatcattg tcagaccgct acctgccacc
    27241 gcgtcctgag tgaaatcgac gccgaagtag cttccccagg tcattcccat cgcgttgatg
    27301 aaatagttcc aggtcaggaa actcaccatc accgccagac aagcacgccc ctgcgcttgc
    27361 ttagcaaggc caatgggtaa accgacagca aaaatcagcg gcatattacg gaacaccgtc
    27421 caaccgccct cttcaataat gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt
    27481 gattccccga caaacatcgg gttttgcagc aagatggcaa gacccaccac aatcccggcg
    27541 aagggaaaca gcagcactgg cgtgaacatc gcgccgccaa agcgttgaat ttgactgagc
    27601 atttccacat cctcttttct caattctgta agtaggaagc ctgattggtt atgcggcagg
    27661 cctggagcga ggataggaag atgcgatcgg cagaacatga cgacccaatg ggattcgtga
    27721 tcgctttcat gcttttcgtt gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc
    27781 tgtgagcaca ggtacacagc atcgaacaca tctttaaaaa aaagatgttt tttcaatcga
    27841 ttaagcagaa cttgtgggcg cattacccgt gcttgcaggc aaaaaagaga tctagagatg
    27901 atctacaaaa gcattgcgga gcggttaaga attcgactta attccgcaga tttcacgcta
    27961 aatagccttc ttcccggtga aaaaaagttg gcggaagaat ttgcggtatc gcggatgacc
    28021 atccgtaaag ccattgacct gctggtagcg tggggattgg tggtccgccg ccacggcagc
    28081 ggcacttacc tggtgcgcaa agatgtactg catcaaaccg ccagcctgac cgggctggtg
    28141 gaggtgttaa aacggcaggg aaaaacggtc accagccagg tgctgatttt tgaaatcatg
    28201 cctgcgcctc cggccattgc cagccagtta cggattcaaa tcaacgagca gatctacttc
    28261 tcccgtcgcg ttcgttttgt ggaagggaaa ccgctgatgc tggaagacag ctatatgccg
    28321 gtaaaactgt tccgtaatct ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt
    28381 gaacaagagt gcgggatttt gattggcggt aattatgaaa gcctgacgcc agtgctcgcc
    28441 gatagactgc tggcgcggca aatgaaggta gcggaacaca cgccactgct gcggatcacc
    28501 tcgttgtcat atagcgagag cggggagttt ttgaattatt cagtgatgtt cagaaatgcc
    28561 agcgaatacc aggtggagta ccatttacgg cgactccacc cggaaaagag ttaaccgata
    28621 ctccagaaga gcaccgccag taattggggg gtgataatgc gcaggaacat cactaacgga
    28681 tagacagtgg cgtaagagag cgccgccgca ccgctggttg gatgaagatt attagcaaac
    28741 gccagcgccg gaggatcggt catggaacct gccagcatcc cgcacatggt caggtaattc
    28801 attttggcta acatccgcgc cagaatgcca acagtaatca gcggaacggc ggtgatcagg
    28861 gcaccataac caatccagct tagcccttcg ccattgacca gggtattcac aaaatctcca
    28921 ccagatttca gaccaacgac cgagaggaac agcacgatcc ccagctcccg cagcgcgagg
    28981 ttggcacttg gcggcataaa ccagtacagc ttgccgatac tgccgatacg cccgaggatc
    29041 aacgccataa tcagcggacc gcccgccagc cccagtttca acgcggccgg gaacccaggc
    29101 acaaagacgg gaatagaacc taacaatacg ccaagcccga tgccaataaa cactggcagc
    29161 atctgaacct gttgcagttt ttgctgcgca ttccccagca cattggcaac ggcatcaatt
    29221 gcggacggac gccccaccag attgaggata tcgccgaact gcaggctgat atcgccactg
    29281 gcgaccagtt cgaccccggc acggttcagg cgcgagataa caacgtcata gcgttcttta
    29341 aagtgcaggt cgcgaatacg ttttccgagc acgttttcat tggtgaccac cacacgctcg
    29401 acgcgcaaat cagtgccttt cgtggacagc gaagtatcga cctcctgacc aatcaccagt
    29461 tgcgcattat gtaaatccgc tggctgcccc accagatgca gcaaatcgcc cagttggata
    29521 atggtatctg gcgaaggaac ttttagggtt tcttcgcgtt tcagacgcga gcagataatt
    29581 ttgtcgccgt tgagaatcgg tacatcttta atggctaaat catgcaggtt agggttctca
    29641 acgcgaatat tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc
    29701 tgagcttctg tctcgacatt gacgcggaaa ataacccgca acatccacat ggtgaacaaa
    29761 atcccgcaaa tgccgaatgg atacgccatc gcgtaactca tccccatctg atcgaccatt
    29821 tccattggtg tacccaagtc gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg
    29881 gtaaccgcac cggagaaaat ccccagcact accggcagtg gaatatcaaa cagtttatgc
    29941 aggatggcgg taaccagacc accgatgatg acgatcagaa cagcaaacag gttgaggcgt
    30001 aatccggaga cgcgcaatga ggcaaagaag cccggcccta cctgaatccc gatagtataa
    30061 acgaacagga tcaggccaaa ttcctgaata acatgcagca tatcgctact taatgtcatc
    30121 cccgcctgag aaacaaaatg gccgacgatg atcccaccaa acagcacgcc gccaatacct
    30181 aatcctatgc cgcgaaattt gacattgccg ataaacaaac cgacgactgc caccaaagcc
    30241 agaatactga ccgttaatgc tatatcactc atcgtccatt tctcttgaga taacattttt
    30301 aattgctacg gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca
    30361 cgggaaagaa cgtgccgaaa tatcttaaac agtcgaaatt taacgcattg ttgagtaagt
    30421 aagacaaaaa aagccccacc agaatggcgg ggcaaagaga atagctagtt agctatttaa
    30481 cgcgggacgt tcgctgatag cgatacgctg cgctgcgatg ggttcaggct cattacgaat
    30541 taaatcaata tgcagtaaac cgtttacgaa ggttgcgcca gagacttcca tattttcagc
    30601 cagcgtaaag ctcaggctaa atggctgatt cataagccct tgatgcagcc attttttctc
    30661 ttcttttggc tgctccggcg tgccttttac gctcaggcgc gtaccttcca gttgaatctc
    30721 taaatcttcc tgacggaaac ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct
    30781 tttctcaatg ttgtacggcg ggaagctctg gctttcaccg gcgttttgca gtgcgttggc
    30841 cagtttgtca aaaccgatcc attgacgcat cagtggggat aaatcgaagt tacgcatagt
    30901 catttctcct tctaagaagc gagtaagtac ctgcaaatcc gaagattcgc atatgctccc
    30961 taacggcgag catggagatg tcaggccgcg ccaggcggcc ttagggaatt agttgatttc
    31021 gatacggcgc ggttttttcg cttccggaat cacgcgttcg agatcgatat acagcaaacc
    31081 atttaccagg ttagcgccac gaacatgaat gttctcagct aactggaatt tgcgttcaaa
    31141 gttgcgttca gcgatgccct ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc
    31201 acctttcacc actagcagat tatcctgggc ggtaatttcc agttcgctct cagcaaaacc
    31261 agccacagcg atagcaatgc ggtaatggtt ttcgtctacc agttcaacgt tatacggagg
    31321 gtagccgcca ttactctggc tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc
    31381 aatagcagaa cggtaaagcg gggataaatc aaagttacgc ataatcaata gctcctgaaa
    31441 tcagcgagaa tgtaagacct tccacaatgg acaggtcagg tagccagaac acccatcagg
    31501 cgcgtcctca tcggctacga tgtaaaaatg ggtctggaaa tgaacttttc aagccttatc
    31561 agcggacttt tttttgcagt ttatggtcta ttgcatagac tgagggggca gcacgaaacg
    31621 ttaaagtgcg atagccgcca cagtgcgacg taatgatgat gttattttca gcaagggatc
    31681 gctataactc atcaggccaa cacccacgat caacagatga atttattatg ataagaaatg
    31741 tgttgttagc gttcatgata tgcagcggaa tgacattact cggaggatgc tccagcgtga
    31801 tgtcccatac cggagggaaa gaaggaacgt atcccggcac ccgcgccagc gcaaccatga
    31861 ttggcgacga tgaaaccaac tggggcacta agtcgctggc gattctcgat atgccgttta
    31921 ccgctgtgat ggacacgctt ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc
    31981 gctcacgcgt tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt attccaccgg
    32041 ccagaatgcc tgataactga tttagcgatc agtttcggtg atccacagct ggccaccttc
    32101 catccacgcc agccattgac catccggcga gaagacctcg gcgtccgcag aaggtggatt
    32161 ggcgtggttt tcggttaaat actcaacctc gccgctttgc gcatgggcac aagcaattcg
    32221 attatccagc acaaagccca accattctcc tgacggatgc cagttaaatg cagactgaat
    32281 atccgttttg ttatgggtta actggcgcgg ctcgccgccc tgtggcgaga taagccacag
    32341 ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct gcggattaca
    32401 gcgcacccag tggcgcggga cgttgactaa ccccggataa gcccgatggt gggtaaaggt
    32461 taaacgtcgc tgcacgacgc cgcgcggtgg cgcgggcagg gtggtttccg ttccacttaa
    32521 cggcgcatca cctgccgctt tccagccagc ttcatcttgc ggtaactcaa cgataaacag
    32581 ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt
    32641 tcctacccat ccttcttcat aagcacgatt gatttcatcg ctgccaggct gcggcgtggg
    32701 cgtggtttta ctcaccagca cgcaccagtg gctaccgctg tattcacgcg gatgctgctt
    32761 ttgtacgttg accgggccaa acggcgcagc aacaccgacg tttcgcaaat ccagcgccgg
    32821 atcgagttca tgcattacat ggtcgttata ggtaaagctc accctttcac cgttcgggct
    32881 aaagacatgc acatggctgc cgccgcgcag cgctcctggc atatacggcg cggtaatatc
    32941 cattgcatcg agattgctca ccttgccgcc ttcagcaatg actccgcgtc gatgatggaa
    33001 atcgtaatgc catgtttcat caggattttc cgggccatgg atgaaaacat atttctctga
    33061 cttcggatga acggtcacca cgccgacgtg tgcgccctgt gacgcgcgat agataacctc
    33121 gacctcgccg gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgccccaga
    33181 aggacgcacg tcaaatacca gccactggct gtcgggcgtc caggtattgg tattggtgag
    33241 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggg
    33301 tcccgccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
    33361 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
    33421 atcggcgttg gcgcgaaaat tagcaggcaa aatgcaggtg atcgcgctgg ataaaaagca
    33481 ccagtgtggt actgaaggtt tcagcaaacc ttgcggcggt ctgctggcac cggacgcgca
    33541 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtgatcgcca acccgcagat
    33601 tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
    33661 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt
    33721 tgagatttat cacgacagcc tgtgtcgaaa aatctggcgt gaggatgata aatggcatgt
    33781 catttttcgt gcagacggct gggagcaaca tattactgcc cgctatctgg tcggtgccga
    33841 tggcgcaaac tcgatggtgc ggcgacatct ctacccggat catcaaatcc gtaaatatgt
    33901 cgctatccag cagtggttcg cagagaaaca tccggtgccg ttctactcct gcatctttga
    33961 taattcgata actaactgtt attcatggag tatcagcaaa gacggttatt ttatctttgg
    34021 cggtgcttat ccaatgaaag acggtcagac gcgtttcacg acgctgaaag agaaaatgag
    34081 cgcctttcag ttccagtttg gtaaggcggt gaaaagcgaa aaatgcacgg tgctgtttcc
    34141 ctcacgctgg caggattttg tctgcggtaa ggacaacgcc tttctgattg gcgaagcggc
    34201 aggatttatc agcgccagtt cgctggaggg gattagctat gcgctggata gcgcagacat
    34261 tctgcgttcg gtgttactga agcagccaga gaagctcaat accgcttact ggcgcgccac
    34321 ccgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
    34381 ggctttaaga aagcggatta tgcgcagtgg tatggcgcat attccacagt tgaaagatta
    34441 gccaacgcgc ttcacatcgc ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
    34501 ggcggagata taaaccagtg caggtgcgaa accgtaaccc tgcgccaggt accccaccac
    34561 cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta aacacgccgc cggttaaacc
    34621 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt
    34681 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttagc
    34741 gcccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
    34801 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccgac
    34861 aaacgccgcg aggaatggca ccgtggtcat aaagcctgct ttcagcgccg tgattccttt
    34921 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgtag aagccaccgc
    34981 aaattgccca agataaacgc cgatcagttt acgatggaac accagtttcc agtctttggc
    35041 tgttaacggc tgacgcgcct ctttcttcac cggcgcatca ccatccacca gaccgccgcc
    35101 atcacgaatg taatccagtt cagctttgct gatacctttg gtcaggcgcg gcggctgata
    35161 aaccttaaac caaatcagcg accagataat gccgatacca ccagtgacaa tgaacaccca
    35221 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcaaaaacg ccagaccgac
    35281 aaactgacca gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct
    35341 ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcacg
    35401 cagaccaatt aatgacatta atcccgtggc aaagccctgg aataaagtcg ccactgacca
    35461 accaaatatc gcaataaaat aagtcacgcg agaacctacg cgatctaaaa accaaccgcc
    35521 ggggatctgg catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttccgc
    35581 tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc acggccaggt tagcgcggtc
    35641 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
    35701 cggctttgct gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc
    35761 gaagccgctc accatgccct gtaatttaca gagggttata tttttgtatt gctgttttag
    35821 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
    35881 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
    35941 tcgatttcca cacctaagcc cggtttcgtt aacggtttaa agaagccgcc aaccatactg
    36001 aagtcttctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
    36061 atactttgtt cctgaagtac cgcgttatag gaaacaaagt cgatatgcag gcaagctgcc
    36121 agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca catcataggc ttctgccatt
    36181 ccagcaattt tgtagcattc ggtaataccg cctgcatggg agagatccgg ttgcagaatc
    36241 gaaataccgc ctgcctccag cacgcgttta aaatcgaaac gtgagaacat gcgctcacct
    36301 gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt agtattcggc ttgttccgcc
    36361 agcaccggct cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc
    36421 gccattggcg cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa
    36481 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
    36541 agccccagtt cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgcagggtt
    36601 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
    36661 ttgtcgcgaa ccaggccgcc catcagttgc cagaccggcg cattcagcac tttgcctttg
    36721 atatcccata acgcctggtc gatcccggca atggcgctca tcaggattgg accgccacga
    36781 taaaatccgg cgcgatacat cacttgccat aagtcattga tgcgcgaagg atcctgacca
    36841 atcaaatagt cacccagctc gtgaactgcc gcttccaccg tacgggcgcg gccttcaatc
    36901 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    36961 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    37021 taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga ctgcccggcg cgatagagat
    37081 cgctgcctaa gcccgcccca gcacaacctg cgtctatcca ctgcgccagg ttttctggcg
    37141 tcacgccgcc aacggcaaag actgcgatgt ccgatggcaa taccgctttt aacgctttga
    37201 tgtattgcgg accaaaagcc gatgacggaa atattttcag cgcctgcgcg cccgcttcga
    37261 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca gacggtcatg ccgtagccca
    37321 ccgcacggcg gatcacttca ctatggatat tgggcgtaac gatgagctga caacccatcc
    37381 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    37441 cgccatacgc atcaacgatg gcgggaatgc tttgctccca ttgtggggaa ttcagcggga
    37501 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    37561 gcttaatgcc gcgcaaaatg gcgatcagcg gaagtttagt ttgccactgc atgagcgatg
    37621 ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgtcacgtc gcaacccatc
    37681 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg
    37741 tgttgatggg tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag
    37801 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    37861 aaaagctgcg gcaatatggc gggcgcatta aggccgcgct caaggccagc cgcgaaggca
    37921 tcggcagagt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    37981 tgatgtaatt caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc
    38041 acccatttgc aatgcgtacc gggcataaca taaagagagg aaggagccag agttcgcgcg
    38101 ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt tatcgtcatg agagacgcat
    38161 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    38221 aaacgggcag gaacagataa atacggagca actttccagc cgacattgct gccgaccatt
    38281 cctgccatta ccactggcgt attctcttca cgccagtcgg tcgtgacttc tgctaacacc
    38341 gcagccggag attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctctccagg
    38401 cagtggtcgc cctgataaag ccaggcgcga agattggtcg atccccagtc aattgcgatg
    38461 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg
    38521 ccgcctgctc tgccgcatcg ccgtcctgat gccgtattgc atcgaacagc gccttatgtt
    38581 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    38641 gctgcaacga actgatcgca atgctaagtt gctgtaacac cgggttatgt accgactgca
    38701 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg
    38761 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaagtc gcgcgctctg
    38821 ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa
    38881 gccgtgggtc gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    38941 actggttacg cggtgccaca aacgcgccgc gataacgttt catttcaatc agccgtttcg
    39001 ccatcaacga acggaacacc tcacggatga tgttgcgcga ggttgcaaac tcttcacaga
    39061 gctccgcctc agccggaagt ggcgagccag gtacgtattt gccgtgaaca atctgtttac
    39121 ccagcgtaat gacaatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    39181 atgttcttct ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt
    39241 gatgacgttc gcgtttgccg tgtgcgtaat gtagtacaaa cttatattgt tgtactacaa
    39301 tttagatcac aaaaagaaca atgcataaaa aaatgacatg cgtcgggcag aaatctgaaa
    39361 agggatatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact
    39421 cgtttaaagg attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttat
    39481 tcccactggc gctgatgctt tcaggctgta tctcgtatgc tctggtttcc cataccgcaa
    39541 agggtagttc aggaaagtat caatcgcagt cagacaccat cactgggcta tcgcaggcaa
    39601 aagatagtaa tggaacaaaa ggctatgttt ttgtagggga atcgctggat taccttatca
    39661 ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc agcacttaac cggcacaata
    39721 ttcaggttgc cgatgacgca agatttgttt taaatgcggg gaaaaagaaa tttaccggca
    39781 caatatcgct ttactaccac tggaataacg aagaagaaaa ggcactggca acgcattatg
    39841 gttttgcctg tggtgttcaa cactgtacca ggtcactgga aaacctaaaa ggcacaatcc
    39901 atgagaaaaa taaaaacatg gattactcaa aggtgatggc gttctatcat ccgtttaaag
    39961 tgcgatttta tgaatactat tcacccagag gcattccgga tggtgtttcc gcagcattac
    40021 tgccagtgac tgttacgctg gacatcatta ctgcaccgct gcaatttctg gttgtatatg
    40081 cagtaaacca ataatcagta agcgggcaaa cgcgtttatg ctgtttgccc gtccactgat
    40141 taattcagca catacttctc aatagcaaac gccacgccat cttcaaggtt ggatttggtg
    40201 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    40261 gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    40321 ttaataccta acacgtcggc cagtgatttc acccccgtac ctttgttaac gcgtttatcg
    40381 aggatttcga ggaagtacgg cgcacttttc agcacggtat atttctcttt cacttcctgc
    40441 ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat caatcatcat cactttcagg
    40501 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat tgtggcaacg
    40561 aaggattcat gcaccgtgta gtagctgata tcacggttgg cggtgtacag cgtggtgcgg
    40621 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acggtagtcg
    40681 tcatagctga gagcggtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    40741 ccgttataag taatgcagta gtcgcccggc agttccatat gcagctcttt caggtaattg
    40801 tgaacacctg cataagggcg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    40861 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggtagcag aagggtgcca
    40921 tccatatcga tagcaatgag tttgatagcc atgagttccc caggtagatt agttcctgac
    40981 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa gggggatgca
    41041 acgcgggttg catgctcccc atttgcttag cggaagagtt tccctttcag cagttccatg
    41101 cctgcggaaa gcagatcgtt attggcttgt ggagacactt caccttgcgg tgagagcgca
    41161 tcaataatct tcggcaattg ttctgccagt aaactggaag ctgtactggt atccacgcca
    41221 agtttttgtc caagatcgga caccgcattt gtgccgagcg ccgattccag ttgctcgcca
    41281 ctaaccgatt gattgccctg ttgattactc agccaggttg agagaatggc ccctaagccg
    41341 ccactttgca gtttttccag cagcacctga atgccgccct gctcctcaac ccaacttaaa
    41401 atagcctgat atttccccgc atcgcctttc agaaaggcac cgacaacttc atcaaaaagc
    41461 cccatgataa tcacctgtaa agcgttacgt gttgacccaa aaagtataga tttgcgggat
    41521 gataattgcg gattgcagaa ataaaaaggg cggagatgat ctccgccctt ttcttatagc
    41581 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaatcatgaa aattcaatac
    41641 attgcaagat tttcgtaggc ctgataagcg tagcgcatca ggcacgctcg catggttagc
    41701 gccattaaat atcgatattc gccgctttca gggcgttctc ttcaataaac gcacggcgcg
    41761 gttcaacggc gtcgcccatc agcgtggtga acaactggtc agcggcaatc gcatccttaa
    41821 cggtaacgcg cagcatacga cggctttccg ggtccatggt ggtttcccac agctgttccg
    41881 ggttcatctc gcccagacct ttataacgct ggatggagag gccgcgacgg gactctttca
    41941 ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg acgacgctcg ccacgttcga
    42001 taaacgcatc ttcttccagc aagccacgca gtttctcacc cagcgtgcag atacgacgat
    42061 attcgccacc ggtgataaac tcgtgatcca gcggatagtc agtatccaca ccgtgggtac
    42121 gcacgcgaac aatcggctcg aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc
    42181 actggctgcc gtgctgttct ttgtcgttca gttcgctgac cagcgcgttc acccagcggg
    42241 taacggtctg ctcatcagag aggtcggctt ccgtcagcgt cggctgatag ataagctctt
    42301 tcagcattgc tttcggataa cgacgctcca tgcggttgat cattttctgc gtcgcgttgt
    42361 actcagacac caatttctct aacgcttcgc cagccaatgc cggtgcgctg gcgttggtgt
    42421 gcagcgttgc gccatccagc gcgatagaga tctggtactg atccatcgct tcgtcgtctt
    42481 taatgtactg ttcctgcttg cctttcttca ctttgtacag cggcggctga gcgatgtaga
    42541 cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa gaaggtcaac agcagcgtac
    42601 gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga taacgcagtt
    42661 tgtccgggtt gtactcgtca cgaccgatac cacagccaag cgcggtgata agcgtcgcca
    42721 cttcctgaga agagagcatc ttatcgaagc gcgctttctc gacgttgagg attttaccct
    42781 tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg
    42841 agtccccttc caccaagtac agttcggaaa gcgccggatc gcgttcctgg cagtctgcca
    42901 gtttgcccgg caggcccgct aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc
    42961 gacgcgccgc ttcacgggca cgggcagcat cgataatttt gccaaccacg attttcgcgt
    43021 cggttgggtt ttccagcagg tattctgcca gcagttcgtt catctgctgt tcaaccgctg
    43081 atttcacctc agaagaaacc agtttgtctt tggtctggga ggagaatttc gggtccggca
    43141 ctttcacgga aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg gcgctaactt
    43201 tggctttttt gctgtagcct tctttgtcca tgtaggcgtt cagggtacgg gtcatcgccg
    43261 cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt
    43321 agatgttttc ctggaagcca tcgttccact gcaacgccac ttcgacgcca ataccgtctt
    43381 tttcagtgga gaagtagaag atattcgggt ggatcggcgt tttgttcttg ttcagatatt
    43441 caacgaacgc cttgatgccg ccttcatagt ggaagtggtc ttctttgccg tcgcgcttgt
    43501 cgcgcagacg aatggaaacg ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca
    43561 gaatttcata ttcgaactcg gtcacattgg tgaaagtttc gaggctaggc cagaaacgca
    43621 ccatggtgcc ggttttttca gtctcgccgg taaccgccag cggggcctgc ggtacaccgt
    43681 gttcgtagat ctgacggtga attttaccct cgcgctggat aaccagctcc agtttttgcg
    43741 acagggcgtt tactaccgaa acaccaacgc cgtgcagacc gccggacact ttataggagt
    43801 tatcgtcgaa tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc
    43861 cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt
    43921 tgtcggcgtg aatggtgacg ataatttctt tacagtgacc cgcgagcgct tcgtcgatag
    43981 cgttatctac cacctcgaat accatgtggt gcagaccggt gccgtcatcc gtgtcgccga
    44041 tatacatacc cgggcgctta cgcaccgcat ccagcccttt caggactttg atactggagg
    44101 agtcataaga attcgacatc aacgtttctc gctcatttat acttgggtta atccgttatt
    44161 ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg acatgtctat aacgtgttca
    44221 gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct ggcaagcaac
    44281 ccacgacgct catcatcaag ctcagaggca aaatcatcta tcaggtagag acaccgccgc
    44341 ccgctttcac gggtgaggaa ctctccttgc gccagacgta aggcgcacat caacagctta
    44401 agctgcccac gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc
    44461 gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc
    44521 agcacctcag catattctgt ctctttctcc cagccgcgct ggaaagagaa agtcagagaa
    44581 aactcaggga gaaattgctt acaggtatcg gccatatcgg ccgcgatacc ggcgctatac
    44641 tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca gttctttatc ccacgggcgt
    44701 agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc gctgcttgag caatcgcttg
    44761 agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat
    44821 gctcttctgt atttggggcc gccgttgagt aaagtaaacc cttctggcgt tatcagctgc
    44881 attggcatca ggtgcgccag ttccgcgact ttatgcccgt cagtaccgtc gatgcggact
    44941 ttgctgtcgc cctgtttgtc cttggttaag ccaatcgccg tctcgcgctc ttcgccctgt
    45001 aatcgcccat ggagaacaaa tgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa
    45061 ctgcgaaacg cccgaccatg gccgagcgta tagatggctt ccagcacgct ggttttgcca
    45121 ctgccgttgg cacctaccag aaagttaaag ccgggagata aagcgagatc cgcggtttca
    45181 atgttgcgga aatcgcggat caacaagcgg gtgagggaca ttacagtctc attggcatga
    45241 caacataggc agccgactgt gatgcggcat cttcaatctg cacgctggaa accgaatcgg
    45301 tcagcatcat gcggacgttt tcgcatttca gcgcgttcag aacatccagc acatagctga
    45361 cgttgaagcc gatttccatc tccgcaccgc tataggtaac gtcgaggatc tcttccgctt
    45421 cttcctgttc cgggttgttg gcggtgattt tcagctggtt ttcgctgaca tacaggcgca
    45481 cgccgcggaa tttctcgtta gagagaattg ccgcacgggc aaacgcctgc ttgagcagat
    45541 cgcagccagc ttccagatgt ttgtccggat tcttcggcag aacgcggcgg taatccggga
    45601 agcgaccatc caccagtttg gaggtgaaga taaagtcgcc aacgtgggcg cgaatattgt
    45661 tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc gagcatacgc atcagttcaa
    45721 tcacgccttt acgcggtacg atcaccgaat ggcttggcaa agattgacca attggcattg
    45781 aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg cagttcttca ccttcggttt
    45841 caaacagcat accatttaag taatagcgaa cgtcctgatg cgccatcgaa aactgggtcg
    45901 cttcaatcag acgcttcatc gttgcctgcg gcagggtaaa ttcgacttca ctctgccagt
    45961 catcgaggtt cgggaaatcc gccgctggca gggtagacag cgaaaaacgg ctacgcccgg
    46021 agcgtaccag catccgttca ccttccagct gcacggcaat ttccgcgcct tcaggcagac
    46081 cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct
    46141 gaaccagcgc aacacgtgcc accatttcca tctcgagatc agtaccggtc agcgacaacg
    46201 taccgtcagc aacctgtaac agcagattac cgagaatcgg tagcgtagga cgaccaccta
    46261 acggaccgct cacctgttgt agcggtttta ataaatgctc acgttctacg gtaaatttca
    46321 taggtttacg atgacaatgt tctgattaaa tttgaaaaat cttctttgat atcgtggctc
    46381 tcttcacgca actgctcgat cttacggcag gcatgaagca ccgtcgtgtg gtcacgacca
    46441 ccaaacgcat cgccaatctc cggcagactg tggttagtca gctctttcgc cagcgccatc
    46501 gccatctggc gcggacgcgc caccgagcgg gatcgacgct tggaaaggag atccgcgact
    46561 ttgatcttgt agtactccgc caccgtcttc tgaatattgt cgatggtgac cagtttttcc
    46621 tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg
    46681 gtaaagttgg cattggcaat gacgcggttc agcgcccctt ccagctcacg tacgttagat
    46741 cgtagacgct tggcgataaa gaacgccact tcgcccggca aacgaatgtc gttttcgtcg
    46801 gcctttttca tcaggatcgc cacacgggtt tccagctctg gcggttcgat tgccacagtc
    46861 agtccccaac cgaagcggga tttcaaacga tcctcaacgc cgttgatctc tttcggatag
    46921 cgatccgagg tgagaatgat ctgttgatta ccttccagca gggcgttgaa ggtatggaaa
    46981 aactcttcct gagatcgttc tttattagca aaaaactgaa tatcgtcgat cagcagtgca
    47041 tctacggaac ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag ggctttaacc
    47101 atgtcctgaa caaagcgctc ggagtgcata taaaccactt tggcattcgg cttgcgcgcc
    47161 ataatgccgt tacccaccgc atgcagcagg tgagttttac ccagacccgt gccgccataa
    47221 aggaacaacg ggttataggc accgccaggg ttatccgcca cctggcgagc cgccgcgcgc
    47281 gccagttggt tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta
    47341 gaacgatagg tcggttctgc cggggccggg atgttatccc aacctgagcg cgtagaaggc
    47401 gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact
    47461 gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt caaaacgcag ctgtggggca
    47521 tccgctccgc agaaactggt tagcagtcca ttgatattat taaggtactt gtcccgtacc
    47581 caatcgagga caaaacggtt tggcgcgtac agggccagcg tgttatcgct cagttccgcc
    47641 tgcaatgggc gtatccacat actgaattct gtggctggta actcatcctg caatcgggca
    47701 agacactgct gccaaagcga aagtgacacg gcggactcca ctcgaacaaa agtcgataat
    47761 gactaaggct gaaacattca tgattgttga cgtacgtcga aaagaccctg cttgagggtg
    47821 acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac
    47881 cgcggatcat agcctaaact gcgcaagaga tcttctgttt ctcacagatt ttcccgattt
    47941 atccacagga ctttccagac ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga
    48001 tttacgccgc atattggaaa atttaatgac catagacaaa aattggctta atcgatctaa
    48061 taaagatcca ggacgatcct tgcgctttac ccatcagccc gtataatcct ccacccggcg
    48121 cgccatgctg gttttcactg gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa
    48181 agcgcggtaa ataaggaaag agaattgact ccggagtgta caattattac aatccggcct
    48241 ctttaatcac ccatggcttc ggtgtccatc gtttcatttt tcggcggata tccaataaag
    48301 ccattgaatt tattcaagtt taggtagaaa tcgccatgaa acgcactttt caaccgtctg
    48361 tactgaagcg caaccgttct cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc
    48421 aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa
    48481 gctaacccct gagtggttaa gctcgcattt cccagggagt tacgcttgtt aactcccagt
    48541 caattcacat tcgtcttcca gcagccacaa cgggctggca cgccgcaaat taccattctc
    48601 ggccgcctga attcgctggg gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt
    48661 cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc
    48721 caacatgaac tcccggctat ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc
    48781 gataaccgtg ctctctcgga agcgttggaa aaattatggc gccgccactg tcgcctggct
    48841 cgcgggtcct gatagccctc attcgggtct atcaacgcct gattagtccg ctactcgggc
    48901 cgcattgtcg tttcactcca acctgttcaa gctacggaat tgaggcattg cgcaggtttg
    48961 gagtgataaa aggcagttgg ttgacggtga aacgcgtatt aaaatgccac cctttacacc
    49021 ctggtggtga cgatcccgtc ccgcccggac catttgatac cagagaacac taacgatgga
    49081 ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg tctttcatga tctggcaagc
    49141 ctgggagcag gataaaaacc cgcaacctca ggcccaacag accacgcaga caacgaccac
    49201 cgcagcgggt agcgccgccg accagggcgt accggccagt ggccagggga aactgatctc
    49261 ggttaagacc gacgtgcttg atctgaccat caacacccgt ggtggtgatg ttgagcaagc
    49321 tctgctgcct gcttacccga aagagctgaa ctctacccag ccgttccagc tgctggaaac
    49381 ttcaccgcag tttatttatc aggcacagag cggtctgacc ggtcgtgatg gcccggataa
    49441 cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa gacgcttatg tgctggctga
    49501 aggtcaaaac gaactgcagg tgccgatgac gtataccgac gcggcaggca acacgtttac
    49561 caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa
    49621 cgctggcgag aaaccgctgg aaatctccac ctttggtcag ttgaagcaat ccatcactct
    49681 gccaccgcat ctcgataccg gaagcagcaa cttcgcactg cacaccttcc gtggcgcggc
    49741 gtactccacg cctgacgaga agtatgagaa atacaagttc gataccattg ccgataacga
    49801 aaacctgaac atctcttcga aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac
    49861 ggcgtggatc ccgcataacg acggtaccaa caacttctat accgctaatc tgggtaacgg
    49921 catcgccgct atcggctata aatctcagcc ggtactggtt cagcctggtc agactggcgc
    49981 gatgaacagc accctgtggg ttggcccgga aatccaggac aaaatggcag ctgttgctcc
    50041 gcacctggat ctgaccgttg attacggttg gttgtggttc atctctcagc cgctgttcaa
    50101 actgctgaaa tggatccata gctttgtggg taactggggc ttctccatta tcatcatcac
    50161 ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg cagtacacct ccatggcgaa
    50221 gatgcgtatg ctgcagccga agattcaggc aatgcgtgag cgtctgggcg atgacaaaca
    50281 gcgtatcagc caggaaatga tggcgctgta caaagctgag aaggttaacc cgctgggcgg
    50341 ctgcttcccg ctgctgatcc agatgccaat cttcctggcg ttgtactaca tgctgatggg
    50401 ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc cacgacctgt cggcacagga
    50461 cccgtactac atcctgccga tcctgatggg cgtaacgatg ttcttcattc agaagatgtc
    50521 gccgaccaca gtgaccgacc cgatgcagca gaagatcatg acctttatgc cggtcatctt
    50581 caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg tactatatcg tcagcaacct
    50641 ggtaaccatt attcagcagc agctgattta ccgtggtctg gaaaaacgtg gcctgcatag
    50701 ccgcgagaag aaaaaatcct gattcggtga gttttcgcta aaataagggc ggtcagttga
    50761 ccgccttttt tcttttcgta gggcggataa gcaccgcgca tccgccacac aaagcaacag
    50821 gaacatcatg agcgataatg acactatcgt agcccaggcc acgcctccgg gacgtggcgg
    50881 tgttggcatc ctgcgcatct ccggcctcaa agcccgtgaa gttgccgaaa ccgtgctggg
    50941 taaactacct aagccgcgct acgccgatta tttgccattt aaagacgccg acggcagcgt
    51001 gctcgatcag gggattgcgc tatggttccc tggcccgaac tcgttcaccg gcgaagatgt
    51061 gcttgaactg cagggtcatg gcggtccggt gatcctcgac ctgctgttaa aacgcattct
    51121 gaccattccc ggcctgcgga ttgctcgccc tggtgagttt tccgaacgcg cgtttcttta
    51181 cgataaactc gacttagccc aggctgaggc gattgccgat cttatcgacg ccagctcgga
    51241 acaggcggcc cgttcggcac ttaactcgct gcaaggcgca ttctccgcac gggttaatca
    51301 tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa gcggcaattg atttccctga
    51361 tgaagagatc gatttcctct ccgacggcaa aattgaagcc cagctcaatg acgttattgc
    51421 cgatcttgat gcagtgcgtg ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa
    51481 agtggtgatt gccggacgtc ctaacgccgg taaatcgagc ctgttaaacg cgctggctgg
    51541 ccgtgaagca gcaatcgtaa ccgatatcgc cggaactacg cgtgacgtgt tgcgtgagca
    51601 tatccacatt gacggaatgc cgctgcatat catcgacacc gccgggctac gtgaagccag
    51661 tgacgaagtg gaacgtattg ggatcgagcg cgcgtggcag gaaattgaac aggccgaccg
    51721 ggtgctgttt atggtcgatg gcaccacaac agacgccgtg gatccggcag agatctggcc
    51781 ggaatttatc gcccgtctgc cagcgaaact gccgatcacc gtggtgcgca ataaagccga
    51841 tatcaccggc gaaacgctgg gaatgagtga agtgaacggt cacgcgttaa ttcgtctctc
    51901 ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat ctcaaacaga gcatgggctt
    51961 tgacaccaac atggaaggcg gtttcctggc gcgccgtcgc cacttacagg cgctggaaca
    52021 ggcagcggag catttgcagc aaggtaaagc gcaactgtta ggtgcctggg cgggtgaact
    52081 gctggcggaa gagttgcgcc tggcacagca gaacttaagc gaaatcaccg gggaatttac
    52141 ttcagacgac ctgctggggc ggattttctc cagcttctgt attggtaagt aaccgcgctt
    52201 acgaagccgt attctgactg tcagatgcgg cttcgcttca ttgttagcac tcctgttatt
    52261 cctcaaccct ttttttaaac attaaaattc ttacgtaatt tataatctta aaaaaaagca
    52321 tttaatattg ctccccgaac gattgtgatt cgattcacat ttaaacaatt ttagaataga
    52381 caaaattctt gagtattata ctgtagcctc gtgtctttcg aggataagtg cattatgaat
    52441 atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg acaataagat tgtcgatcac
    52501 cgcccttaat ttacccttct gtagccatca ccagagccaa accgattaga ttcaatgtga
    52561 acgattagtt tgctatatct taattttgcc ttttgcaaag gccatctctc gtttatttac
    52621 ttattttagt aaatgatggt gcttgtatat atacatggcg aattaatgga tattgcagat
    52681 gtaatattca cagggatcac tgtaattaaa ataaatgaag gattatgtaa tggaaaactt
    52741 taaacatctc cctgaaccgt tccgcattcg tgttattgag ccagtaaaac gtaccactcg
    52801 cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac ccattcctgc tggatagcga
    52861 agatgtgttt atcgatttac tgaccgacag cggcaccggg gcggtgacgc agagtatgca
    52921 ggccgcgatg atgcgcggcg acgaagccta cagcggcagc cgcagctact atgcgttagc
    52981 cgagtcagtg aaaaatatct ttggttatca atacaccatt ccgactcacc agggccgtgg
    53041 cgcagagcaa atttatattc cggtactgat taaaaaacgc gagcaggaaa aaggcctgga
    53101 tcgcagcaaa atggtggcat tctctaacta tttctttgat accacgcagg gccatagcca
    53161 gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata ctggcgtgcg
    53221 ttacgacttt aaaggtaact ttgaccttga gggattagaa cgcggtattg aagaagttgg
    53281 cccgaataac gtgccgtata tcgttgcaac catcaccagt aactctgcag gtggtcagcc
    53341 ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg atattccggt
    53401 ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc atcaagcagc gtgaagcaga
    53461 atacaaagac tggaccatcg agcagatcac ccgcgaaacc tacaaatatg ccgatatgct
    53521 ggcgatgtcc gccaagaaag atgcgatggt accgatgggc ggcttgctgt gcatgaaaga
    53581 cgacagcttc tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc aggaagggtt
    53641 cccgacatat ggcggcctgg aaggcggcgc gatggagcgt ctggcggtag gtctgtatga
    53701 cggcatgagt ctcgactggc tggcttatcg tatcgcgcag gtacagtatc tggtcgatgg
    53761 tctggaagag attggcgttg tctgccagca ggcgggcggt cacgcggcat tcgttgatgc
    53821 cggtaaactg ctgccgcata tcccggcaga ccagttcccg gcacaggcgc tggcgtgcga
    53881 gctgtataaa gtcgccggta tccgtgcggt agaaattggc tctttcctgt taggccgcga
    53941 tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg
    54001 cgcaacatat actcaaacac atatggactt cattattgaa gcctttaaac atgtgaaaga
    54061 gaacgcggcg aatattaaag gattaacctt tacctacgaa ccaaaagtat tgcgtcactt
    54121 caccgcaaaa ctgaaagaag tttaattaat actacagagt ggctataagg atgttagcca
    54181 ctctcttacc ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac
    54241 tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt atggttatag caggtacagt
    54301 aattggtgga ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg
    54361 tgcctttatc cttatcattg cctggttttc aatgcttcat tccgggttat tgttattaga
    54421 agcaaattta aattatcctg tcggctccag ttttaacacc atcaccaaag atttaatcgg
    54481 taacacctgg aacattatca gcggtattac cgttgccttc gttctctata tcctcactta
    54541 tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta
    54601 tcacgctaat ccacgtattg tcgggatctg cacagccatt ttcgttgcca gcgtattgtg
    54661 gataagttcg ttagccgcca gtcgcattac ctcattgttc ctcgggctga agattatctc
    54721 ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat tactccatcc tgcgcgatgc
    54781 caccagcacc actgcgggaa cgtcttactt cccgtatatc tttatggctt tgccggtgtg
    54841 tctggcgtca tttggtttcc acggcaatat tcccagcctg attatttgct atggaaaacg
    54901 caaagataag ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg tgatttatct
    54961 cttctggctc tattgcacga tggggaatat tccgcgcgaa agctttaagg cgataatctc
    55021 ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat
    55081 tatcgagttt tgcctgctgg tgttctctaa cttagctgtt gccagttcgt tctttggtgt
    55141 cacgctgggg ttgttcgatt atctggcgga cctgtttaag attgataact cccacggcgg
    55201 gcgtttcaaa accgtgctgt taaccttcct gccacctgcg ttgttgtatc tgatcttccc
    55261 gaacggcttt atttacggga tcggcggtgc cgggctgtgc gccaccatct gggcggtcat
    55321 tattcccgca gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga tgttcacggt
    55381 ctggggcggc aatcttattc cggcgattgt cattctcttt ggtataaccg tgattttgtg
    55441 ctggttcggc aacgtcttta acgtgttacc taaatttggc taaatccttc aagaagccag
    55501 ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt ccaaatggca actcgcctga
    55561 ttctccttca ccacgtatgc tttgcgtcac cttactatca ggacgcttta gcccatgtcc
    55621 cgctttttaa tttgtagttt tgccctggtt ttactttatc ccgccgggat tgatatgtac
    55681 ctcgttggtt taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt
    55741 gcgttctccg tttatctggc gggcatggca gctgcgatgt tatttgccgg taaagtggcc
    55801 gatcgttcag ggagaaagcc tgtcgccatc cccggcgcgg cgctatttat tatcgcttcg
    55861 gtgttctgtt cactggctga aaccagcacg ttatttcttg cagggcgctt tctacagggg
    55921 ttgggcgcag gctgttgtta cgtggtggcg ttcgccattt tgcgcgacac gctggatgat
    55981 cgacgtcggg ctaaagtgct gtcgttactc aacggtatta cctgcatcat tccggtgtta
    56041 gcgccggtgc tcggacacct gattatgctt aaattccctt ggcagagtct gttctggacg
    56101 atggcaatga tgggcatcgc ggtactgatg ttatctttgt ttattttaaa agaaacgcgc
    56161 ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata gcgagtcgct gcttaatcgg
    56221 tttttcctca gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct cactttcgtc
    56281 aatacatcgc cggtattgct gatggaaatc atgggttttg agcgcggaga atacgccacc
    56341 attatggcgt tgaccgctgg cgtcagcatg accgtttcat tctccacgcc atttgcgctg
    56401 ggaattttta agccacgtac gttgatgatc acctcgcagg tgttattcct tgcggcgggg
    56461 atcacccttg ccgtttcacc ttcccatgcg gtttctctgt ttggtatcac gctgatttgc
    56521 gccggtttct cggtaggttt tggcgtagcg atgagtcagg cgttaggacc attttcatta
    56581 cgcgcgggcg tagccagctc gaccttaggt attgcgcagg tttgcggttc gtcactgtgg
    56641 atttggctgg cagcggtggt tggtatcggc gcatggaata tgctgatcgg gattctgatt
    56701 gcctgtagca tagtgagcct gttactgatt atgttcgtcg cgcctggacg ccccgttgcc
    56761 gctcatgaag aaatccatca ccacgcttga tctcaatctg ctgctctgtc tgcaactgct
    56821 gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc cttcagcggt
    56881 gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc
    56941 gctggggctg tcgcccacac cgctaatggt cagcatggag caaaatctgg cggaatggat
    57001 gcaaatgagc aatctgctgc tggataaacc gcaccaccag acaccgcgcg gcctgaagtt
    57061 tgagttggcg gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt cgaaacggat
    57121 ctaccaacgt tacccgcagg cgaccatcaa actacgtaac tgggattacg attccttaga
    57181 tgccattact cgtggtgaag tggatattgg tttttccggt cgcgaaagcc atcctcgctc
    57241 gcgggagctg ttaagctcgc taccgttagc cattgattat gaagtgctgt ttagtgatgt
    57301 accctgcgtc tggttacgca aagatcatcc ggcactgcat gaaacgtgga atctggacac
    57361 ctttttacgt tatccgcata tcagcatttg ctgggaacag agcgacacct gggcgctgga
    57421 caatgtgtta caggagctgg ggcgcgaacg cacgattgct atgagcctgc cggaatttga
    57481 gcagtcactg tttatggcag cgcaacccga caatctgcta ctggcgaccg cgccgcgcta
    57541 ctgtcagtac tacaatcaac tccatcaact gccgttggtt gctcttcctc tcccgtttga
    57601 cgaaagccag caaaaaaagc tggaagttcc ttttaccctg ctgtggcata aacggaacag
    57661 ccataatccg aagatcgtct ggttacggga aaccattaaa aacctttacg cgtcgatggc
    57721 ataaccgaat cgtatgaaac gcgacccaat ttcacaataa aatgtaaaaa agttgtaata
    57781 agcttgtctg aatcgaactt ttagccgctt tagtctgtcc atcattccag taaatgatta
    57841 ctcttatatt cataatggac cattaagcat ggagcgaaaa atggcgactc actttgcccg
    57901 agggatttta acggaaggac atctgatttc tgttcgtctc ccctcccagt gtcatcaaga
    57961 agcccgaaac attccccctc atcgtcaaag ccgttttctg gcgtccagag gtttactcgc
    58021 agaactgatg ttcatgctgt atggcattgg cgaattgccg gaaatcgtca ccctgccgaa
    58081 aggtaaaccg gttttcagtg ataaaaattt gccttcgttt tctatttcct atgccgggaa
    58141 tatggttggc gtggcgttaa caaccgaagg tgaatgtggc ctcgatatgg aactacagcg
    58201 tgcgacgcgc gggtttcata gcccacacgc gcccgataac cacacctttt ccagcaatga
    58261 atcgctatgg atcagtaaac aaaacgatcc taacgaagcg cgggcgcagc tcatcacgct
    58321 gcgccgaagc gtgctaaaac taaccggtga tgttttgaat gacgatccgc gcgatctgca
    58381 gctgctgccc attgccggac gcctgaaatg tgctcatgta aatcatgtag aagcgttatg
    58441 cgacgcggag gacgtgctag tgtggtccgt ggcggtcacg cccgcgattg aaaagctcag
    58501 tgtctgggag ttagatggca aacacagctg gaaaagcctg ccggatattc acagccgcgc
    58561 caacaatcct accagccgga tgatgcgttt tgcccagctc tctaccgtga agtctttttc
    58621 gccaaattga tagacaacca caggagtcat tatgtctgaa aaattgcagg tggttacgtt
    58681 actggggagc ctgcgcaaag gctcatttaa tggcatggtt gcacgtaccc tgccgaaaat
    58741 tgctccggcg agcatggaag tcaatgcgtt accttccatt gccgacattc ccctgtatga
    58801 cgctgacgta cagcaggaag aaggttttcc agcaacggtt gaagctctgg cggaacagat
    58861 ccgtcaggct gacggtgtgg tgatcgtcac gccggaatat aactactcgg taccgggtgg
    58921 gctgaaaaat gccatcgact ggctttcccg cctgccggat caaccgctgg ccggtaaacc
    58981 ggtgttgatt cagaccagct caatgggcgt gattggcggc gcgcgctgtc agtatcacct
    59041 gcgccagatt ctggttttcc tcgatgcaat ggtgatgaac aagccggaat ttatgggcgg
    59101 cgtgattcag aacaaagttg atccgcaaac cggagaagtg attgatcagg gtacgctgga
    59161 ccacctgacc gggcaattga ccgcatttgg tgagtttatt cagcgagtta agatctaaat
    59221 aaaaaacccg ccagcaatca tgcatggcgg gtttttaacg cgctatcgat tttagtgagc
    59281 gtcgataaag acaatcttca ggataaacag cagcgcaacg atgattacgc acgggcttag
    59341 atcacgcaga cgtccggtac cgattttcat cacgcagtag gagataaagc ccagcgcgat
    59401 accttcggta atcgagaagc tgaacggcat catcacggcg gtaataaacg ccggaacaga
    59461 ttcagtaaga tcctgccagt tcacgcgtgc cagactggaa gtcatcagca cgccaacgta
    59521 aatcagcgca ccagctgcag cgtagcctgg caccatcccc gccagcggcg acagaaagat
    59581 aaccagcagg aacagcagac caacaaccac tgccgtcaga ccggtacgac cgccaaccga
    59641 tacgccggaa gaggactcaa tataagccgt aacggaagaa gtaccgataa acgaaccggt
    59701 cacggaagag atactgtcga catacagcgc ctgcttcatg cgcgggaatt tacccttctc
    59761 atccgccagg cctgctttat cggtcacgcc aatcagcgta ccggaggagt caaacaggtt
    59821 gaccagcatg aaagagaaaa tcacccctgc cagcccgagg ttaaacgacc cggctaaatc
    59881 tacatgaccc acaactgtca ttacgctcgg cggcgcagaa acgatgccat tgtagtgcac
    59941 atcacccagc atccagccca gcagcgtcgt caccacgata gaaaccagca ccgctgcgtg
    60001 aatgttgcgc gaggccagaa tagcaatgat gaagaagccg aggataccca gaagtacgct
    60061 gtgagaagtc agattaccga tgctcaccag cgtttccggg ttagcgacaa tcacacctgc
    60121 gtttttcagc cccatcatgc caatgaacag accgataccg ctggtaatac ccacacgcag
    60181 actcaccgga atgttggcaa tcatccagta gcgaacgcgg aaaatcgtca gtaacagcag
    60241 acctatcgcg ccccagaaga ttgcgcccat cccgacctgc cacggcaagc ccatcgcctg
    60301 tacaacgaca aaagcgaaga acgcattcag gcccatagcg ggtgccagtg caactggcag
    60361 gttagcaaac agtcccatca taatactgcc gaatgcagcg atcagacagg tagtgacgaa
    60421 gacggcgctg gtatccatgc cagcaacgcc aagaatttgc gggttaacaa aaacgatgta
    60481 aaccatcgtc aggaaggtgg taaaaccggc gatcacttcg gtccgtgccg tcgtgccatg
    60541 ttcgcgcagt ttaaacacgc gttccagcat cccctggcca gaagcctggg ttgtgtgttg
    60601 ttgactcatt atctatttcc gaacaaggag ggaaaatccg tcgctatcgt ataccaaaat
    60661 gagacaatat ggacggctga agataacttt ttttcattgt atttgcttat acggcaacga
    60721 ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa cgataaagga aagacatgtc
    60781 ccagatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg aagtcatttg
    60841 ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc ctgaagaggt
    60901 attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt cccttgaaca
    60961 tggtgttacg ttagcgaaaa cagaagctga acacgtttac cgtgcagaag tcgctcggct
    61021 gttcgattca gaactggaag ccatcgaagg ggctggagcg ctcctgtcag cgatcactgc
    61081 gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta tgggcaagct
    61141 gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc agcgctggaa
    61201 gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg tagaaaactg
    61261 cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag gtatggaagt
    61321 gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag tcaccacctt
    61381 tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata ttacggcata
    61441 gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta agtaaaggga
    61501 gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg cggttttagg
    61561 accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag aaccgctggt
    61621 tcttttgcga agaatacagg tacttccatt gtttctctta ttgtcgatta ctacgggagt
    61681 tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga tcgggtcaca
    61741 gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg agatctattg
    61801 tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtatgaaa acattctttc
    61861 tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg tgatgagcag
    61921 aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact ctttaagtta
    61981 agcgggatac tttatctttg ggctactcaa aagcagacag gatgttacta tgactcaaaa
    62041 tatcaggccg ttaccccaat tcaaatatca tcccaagcca ctggaaacag gcgcatttga
    62101 acaggataaa accgtagagt gcgattgctg tgaacaacag acgtcagttt attactcggg
    62161 tcccttttat tgcgttgatg aagttgaaca tctctgtccg tggtgtattg cggacggttc
    62221 tgctgctgaa aagtttgcag gtagttttca ggatgatgcc agcatagaag gtgttgaatt
    62281 tgagtatgat gaagaggacg aatttgccgg tattaagaac acatatcctg atgaaatgct
    62341 gaaagagttg gttgaacgca cgccaggtta tcatggatgg caacaggaat tctggcttgc
    62401 gcattgtggc gatttctgtg cttttatcgg ctatgtgggc tggaatgata taaaagatcg
    62461 cctcgatgaa tttgccaacc ttgaagaaga ttgtgagaat ttcggtatta gaaattctga
    62521 tctagctaaa tgcctgcaaa agggtggtga ttgtcagggt tatctcttcc gctgtctcca
    62581 ctgcggcaag ctgagactgt ggggtgattt ttcgtagtta tttaaataat gagaacaggc
    62641 cggagcgtaa ttcatacatc cggccttatt tcttcagctt aatcgagcgc taattctgcg
    62701 gcagcggctt tatcagcgat taccatcagc gaaggatgca gctgcaacac ggaagctgga
    62761 acatcttcgg taaccggacc ttgcagcaca tttttcagtg cctgcgcttt acctgcgcca
    62821 ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt tcggccccat cgtcacgtag
    62881 ctgtcaggca ctaacgaaaa gtcgccgccc aactcaccat gcgccacaat atcaaccatc
    62941 tctccctgga tcgggaactc cacggtttgc tcatggaaat gggtcgtatt cggcaaattg
    63001 ccacaaaaat gaccatctgc acctaatccc agcaccacca aatccaggcc gccttcacgc
    63061 gccagtttct gatcatgctc gcggtagtta tcaatagtga gcttctggat attctcttct
    63121 ttgatccccg caggggtgaa aaacagatta cgcagattgg taatcgttac accttctccc
    63181 tctttgccgc gaaagggaat ttcatcgaaa ttatagaaat agcagttatc ataccagggc
    63241 ttacctttaa ccagggtggt gaggtattca tacatgcctt tgggcgtgct accggcggta
    63301 attgccaggt taacacgacg cgtcttcgac atataaccaa gcagatggtg tgccgcgaca
    63361 cggctcattt cctggtaatc ttcggtaatg attaatttca tcttagtgcc ttattttatt
    63421 taaataattt ctgtacgaag gctgcatagg caggccgcca gacatccatt tcatggttca
    63481 ggccgggata ttcctggtaa tcaaacttaa tttttttctg ctcaagctca gttttcagcc
    63541 cggcgatatc cttgccggtt acgacatctt tatcaccaac gaccacagta aaattacgta
    63601 gttgctggtt aatagctgcc ggatcgttca gccgggccgc gacaccttca tccggtacgg
    63661 ttgtcgtggt aacaccactg aatgtggcca gccagccaaa gctttccaga tgattcattc
    63721 cggaaaccag cgcctggtac ccgccttgtg aaagccctgc cagcgcgcga ccatcggcat
    63781 ctttacggac attaaaacgc ttgctaatca gcgggataat atcgttcatc agttcgcgat
    63841 ctgccgcttt agcattcagc ggataaaaga ctttacgtct ttcctgaggc acgaaatctt
    63901 cgggaataat gcccttcgca tcggtttcgg tatccgggat caccaccagc atcggtttaa
    63961 ttttcccttc agccagcagg ttatccatga tttgcgggat acgcccctga tcgatagcgg
    64021 aacgtccggt atcaccaaaa ccgtgataga aatagagcac tggcaaaggc tcgcccatgc
    64081 cggtgtaacc tggcggggtc cagacataca tctgacgttc agattgcaat gcgttggagt
    64141 ggtaagttat ggcgatcaaa tcaccatgcg caacagaacg cgtatccaga taactgcctg
    64201 gcaccagaat catactggag ttaacctggc gctgaggctt agtcattgcg gtgcctgtat
    64261 caatgctgcg tacaccatca acattgaaaa aatactcgta caggttgcct ttcaggatag
    64321 gtgtgcgcca cgaccagact cctgcttcgt ctttggtcat cgggtgaata ttgtccggaa
    64381 ccggaacacc caccactaca gagacatttt ttgccccagg ggcaaagtag cgaaaggtca
    64441 cgctgttatc ggcattgacc tgagtcacat actgtttcac cgggatagtg ggtgccgggc
    64501 tggctggcat atccgctgct atggcccact gagcagaaat accgctggca atagccagcg
    64561 ttaaagcagc aatttttata ttcatcgtaa tattcctttc attaagttcc attaccacca
    64621 gatttcagcc tgggcaccga cagcgaactg gtcgtcttta ttgtcttcga aggtgaagcc
    64681 atccagttcg ttttccaggg ctttgatata cgtggcgtag aaacgaattt ccggacgcga
    64741 ggtcaacata ctggtattga ctttaaaggt atggaagagc gtggttttat aaccagactc
    64801 attaaattta ttactattcg catctttgtt ttgctgggtg aaatagccca gttcaacacc
    64861 tgtctggtta tattggtccc aaatataggc cgggcgaaca accgcacgaa tagattcgaa
    64921 atcagagtgt gcgcctgttt cgtagctata aatatcgtta ccgaaggagt aaacaatcgc
    64981 gttagccaca ataaaatgat cgccgatata ggcttcgccc tgtgaagtca gacgaactgc
    65041 tgttccgccg gtgtgatcac cataataacg accattaaag gtggtaaatg gacttgcgcc
    65101 agcataacgg ccaaagttac tggcgataga gttattcgca accaggaagg agaattcgtt
    65161 gaagccacct ttatcaaatt tctgcgttaa agatgtgcca aacatccagg tatctttcca
    65221 gtcataatag ccgttattat cctgattatc tttttccgat gcgctttcgt ttgccgtgac
    65281 ataacgacca ctcaccatta aggtcgcttt atcccataac gggatatctt tatagcgtaa
    65341 atcaatagta ttggtattaa tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac
    65401 atcttcgcga accagcgcga tatcaatttt acccggaccg actttccagt tttccagacc
    65461 tacacccgct gcggcatcag tacgctgcgt tttccagtcc agcatctgga tttcaatttt
    65521 cggcgcaccg tgtttaccca cccagaaatc agcctctggc gcaaagggca ggaaaccttt
    65581 ggtggtaacg tacatatcgg agaactgcat aaagttctcg ccaccggcgt tatcgccaaa
    65641 ccagccggta gagtactgct gaccaacgtt accatccatc atcacaacgg catcaacccg
    65701 tttgccgttt tcgttgtaga cacgttgttt taactgcaaa tcaaaccagc cggagtattc
    65761 gttaccaaag cggcccagag aaccaatcgc ccatgattta ggtgaaccat gagaggcggt
    65821 cccccaacca gaacggtaat agccgttgta actaaagcca atttcatctt tcacaaactt
    65881 actgaaatct ttcagagtca tggaagaata aatcgagcct gtctcactgg catttttctc
    65941 ttcatttttg accagtacag catcaggttt tgcggcacta ctggtcggga acggattggc
    66001 ggcataccct tgatcattcg tacttacgct acgattcacc gtcgctggcg tatatttttt
    66061 cttctcttca tctttatact ttttgagttc gctttgcgtt tctcttaacg ctttttctaa
    66121 taactcaagg cgatgttcca ccgttaatga ttcagccaat gattgtgcag aaaaggctaa
    66181 cggtgccatt aataagatgg cagaggtaat aagatttcgt ctaaacataa tatcccttta
    66241 tggtgcaaag aaagaattaa cgtatcgcat ccagactgtt ctgaatgcga cgataattaa
    66301 ggtgctttat tggttatttt tttaatgaca gcccccgcgt tttgatcact tctgcatacc
    66361 atccgaagct tttcttacgc gttcttgtga ggcttccttc gccattatca tcacgatcca
    66421 cataaataaa gccgtagcgc ttagacattt gtgaatgaga tgcactgact aaatcaattg
    66481 gcccccaact ggtgtacccc ataatatcca caccatcggc aatcgcttca tttacctgta
    66541 ccaggtgatc gtttaaatag gcaattcgat aatcgtcctg tatcgaacca tccgcttcaa
    66601 cgctgtcttt tgcgcctaat ccgttctcga caataaataa cggtttttga taacgatccc
    66661 aaagcgtatt taacagaacc cgtaatccaa ccgaatcaat ttgccacccc cactctgaac
    66721 ttttcagatg cggattgggg atcatattca gtatgttgcc ctgcgcattt ttattaatgc
    66781 tttcgtcgtg ggaaacacaa ccagtcatgt aataactaaa agagatgaaa tcgacggtat
    66841 gttttaaatc ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga
    66901 aacgctgcat atagccggga tactggccac gcgcctgaac atcaccaaag aacatccagc
    66961 gccggttctc ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacca
    67021 gcccaccgag aagcatattg ccgattttcg cttcggggag caggctatga caggctttaa
    67081 ctgcccgcgc actggcaacc agttgatggt ggatagcctg ataaacttcc gcctcgccac
    67141 tctcttctgc cagccccacg cccgtgaatg gctcgtgtaa cgacatgttg atttcattaa
    67201 acgtcagcca taacgccact ttatgttggt agcgagtaaa gaccgtgcgg gcgtaatgct
    67261 cgaagtgatc gatgaccgct cgattagccc aaccgccgta gtttttcacc agcccatatg
    67321 gcatttcgta atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa
    67381 acagccgatc gtaaaacgct aaccccgctt cattcggttc gacttcgtcg ccctgaggga
    67441 aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg
    67501 cgatatcttc cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc
    67561 ccaggatgcg cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgaga
    67621 tccctttgcc atcttcctgc caggcacctt ccacctgatt ggcagctgtt gcgccacccc
    67681 aaagaaatgt ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg
    67741 ataacagcgg ttcacctgcg cttatctgcg ccgtgccgtg gggtaatacg tccgtaaaat
    67801 catcgctatt actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag
    67861 ggatatcaaa agaaatcagc cgatcgcctg tattgacctt gtcacccacg ttgacgtgag
    67921 cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc gacatgaatc aggagctcca
    67981 caccatcatc tgactcaatg ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac
    68041 ccgcaaccgg agaacgcact tcaccaaccg agggcagaat ggcgatacct ttacccaaca
    68101 ggccactggc aaacgtggta tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg
    68161 gtgaacagat accgccctgc tcaggtggtg taataacctc tggtgttttc tcttgcgggg
    68221 caccctgcgc tggctgacgt ttagcggtga tgaaatgaag catcaccgta ccgacaaatg
    68281 cgcaaccgat ggcaatgaca ccgccaataa cgctggccca gacggtgaaa tcaattcccg
    68341 ttgacgggat ggtttgcatg aaggtgaaaa tacttggcaa accaaaggag tacactttcg
    68401 tttgcgcgta gccaatgatg gtggccccca aagccccact gatacaggcg ataacaaagg
    68461 ggtacttacg cggcagatta acgccatata ccgctggttc ggtgatacca aacaggcccg
    68521 tcaacgccgc tgatcccgcc accacttttt tctgcgcatc gcgttcgcag aggaagacgc
    68581 cgagcgccgc cccgacctgc gccataatgg cgggcattaa cagcgggatc atggtgtcgt
    68641 agcccagcac ggtgaagtta ttgatacaca gcggcaccag gccccagtgc agtccgaaca
    68701 tgacgaagat ttgccagaag ccgcccatta ccgcgcctgc aaatgcagga accgcctgat
    68761 aaagccagag ataaccggcg gcaatcagtt cgcttatcca ggttgatagc ggccccacca
    68821 gcagaaaggt gacgggtgtg ataaccatca gacatagcaa tggtgtgaag aaatttttga
    68881 ttgccgacgg taaccacgca ttaagtcggc gttccagaat gctgcacaac caggcagaaa
    68941 aaataatggg aataaccgat gacgagtaat tcaacaatgt gaccggaata cccaggaaat
    69001 ccagccccag ctcatccgct ttttgcccgt tctcgaaagc agtcagaatt aatggatgca
    69061 ctaacgctcc accaatcacc atggcagtaa atggattacc gccgaagcgt ttccccgcgg
    69121 tgtatcccag gattatcggg aagaaccaaa acaaggcatc actggcgctg aataaaatta
    69181 aataagtacc actttgttcg gtcgtccact gaaaagtgag cgccagagcc agcatacctt
    69241 tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt aaaaatacct gaaataacat
    69301 aaacaaagcg gtttagcaga ttacctttct catcattttc cggtgcctgt tgcgcttttt
    69361 cgccaaggcc tgccacgctg ttgaccgcca ggaagacatc ggccacatgg ttacctatga
    69421 ccacctgaaa ctgaccaccg ctttccacca ccataataat accgggggtc tttttcagta
    69481 cctcagcttg cgctttgctt tcatccttta acttaaaacg taatcgcgtt gcgcaatgca
    69541 tcagactcac aatgttatct gcgcccccga ctcctgcgac tatttttctg gctaactccg
    69601 tcataacttg ccctctaccg ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg
    69661 cctgacgtga atcaagcaat ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt
    69721 tactcagtaa taatatttca gtgttctttg cgcacgcgct ctatatttat ggctaaaaac
    69781 ataatctctg cgggtgaaat tttacgttga tactgcaaac caataaaaat ggcgatccgt
    69841 tccgcacatt gccatgcttg cgggtaattt tgttttactg cttgttgtaa tgattcatca
    69901 ctatcgttaa ttgaagcatg ttcaagaata cgccaggata aaaacttcag atgtgtaacc
    69961 agtcgctgat aactcaagct ttcttcctgg taattaaggc tgaactgaaa ttttattaat
    70021 tgcagcattt cgcgcattaa ctgcgtgaca cctgcaacat cctccatatt tccgctcatt
    70081 tgggcactga ccagatgcat ggcaataaag cccacttcat ctttcggtaa ctgcacgccc
    70141 aaccgtttat caataatggt taacgcttct tcccctagct ggaactcttt cgggtaaagc
    70201 cgctggatat cccacagcaa cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc
    70261 gcaaactggc aatggtcagt tagcgagata taaatactgt cctgtaattt tcccaagcgc
    70321 tcctgcgcta aagagataat acgatcacag gttgccatca cctcaagagg aatatgactt
    70381 aagagttcgc ttaatcgccc gttcagttca tgactgctca aggcatactc tttttctatt
    70441 ccacttgagt taattctttc gccagcgcgt ttttgaaagc caattcctcg ccccatgacg
    70501 actttttccc gctgttgatc atcaataacc accacaacat tattgttgag aattttggtg
    70561 atttgcatgt tcatagcaag gacctttttt ataaacaaaa aaacccgact tcaccagtat
    70621 tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca atccagtcat ttattaatgg
    70681 tttttataac gaacatccag gttcggaaat taatttaatt gcgtgcagag aaaatagcaa
//
LOCUS       DAZFDP010000007        65748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFDP010000007 DAZFDP010000000
VERSION     DAZFDP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1584
                     /gene="yqiK"
                     /locus_tag="ACHJ1G_000531"
                     /pseudo
     CDS             1..1584
                     /gene="yqiK"
                     /locus_tag="ACHJ1G_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            1823..1882
                     /locus_tag="ACHJ1G_000532"
     CDS             1823..1882
                     /locus_tag="ACHJ1G_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4762440.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            2199..2258
                     /gene="ibsE"
                     /locus_tag="ACHJ1G_000533"
     CDS             2199..2258
                     /gene="ibsE"
                     /locus_tag="ACHJ1G_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO4762441.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2378..3811)
                     /gene="hldE"
                     /locus_tag="ACHJ1G_000534"
     CDS             complement(2378..3811)
                     /gene="hldE"
                     /locus_tag="ACHJ1G_000534"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4762442.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNITSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3859..6699)
                     /gene="glnE"
                     /locus_tag="ACHJ1G_000535"
     CDS             complement(3859..6699)
                     /gene="glnE"
                     /locus_tag="ACHJ1G_000535"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4762443.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(6722..8023)
                     /gene="ygiF"
                     /locus_tag="ACHJ1G_000536"
     CDS             complement(6722..8023)
                     /gene="ygiF"
                     /locus_tag="ACHJ1G_000536"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4762444.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8265..8885
                     /gene="ygiM"
                     /locus_tag="ACHJ1G_000537"
     CDS             8265..8885
                     /gene="ygiM"
                     /locus_tag="ACHJ1G_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4762445.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            8949..10187
                     /gene="cca"
                     /locus_tag="ACHJ1G_000538"
     CDS             8949..10187
                     /gene="cca"
                     /locus_tag="ACHJ1G_000538"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4762446.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10350..11171)
                     /gene="bacA"
                     /locus_tag="ACHJ1G_000539"
     CDS             complement(10350..11171)
                     /gene="bacA"
                     /locus_tag="ACHJ1G_000539"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4762447.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11261..11629)
                     /gene="folB"
                     /locus_tag="ACHJ1G_000540"
     CDS             complement(11261..11629)
                     /gene="folB"
                     /locus_tag="ACHJ1G_000540"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4762448.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11734..12351
                     /gene="plsY"
                     /locus_tag="ACHJ1G_000541"
     CDS             11734..12351
                     /gene="plsY"
                     /locus_tag="ACHJ1G_000541"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4762449.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12364..13296)
                     /gene="ttdR"
                     /locus_tag="ACHJ1G_000542"
                     /gene_synonym="dan"
     CDS             complement(12364..13296)
                     /gene="ttdR"
                     /locus_tag="ACHJ1G_000542"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4762450.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13503..14414
                     /gene="ttdA"
                     /locus_tag="ACHJ1G_000543"
     CDS             13503..14414
                     /gene="ttdA"
                     /locus_tag="ACHJ1G_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4762451.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14411..15016
                     /gene="ttdB"
                     /locus_tag="ACHJ1G_000544"
     CDS             14411..15016
                     /gene="ttdB"
                     /locus_tag="ACHJ1G_000544"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4762452.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15064..16527
                     /gene="ttdT"
                     /locus_tag="ACHJ1G_000545"
     CDS             15064..16527
                     /gene="ttdT"
                     /locus_tag="ACHJ1G_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4762453.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16570..17583)
                     /gene="tsaD"
                     /locus_tag="ACHJ1G_000546"
     CDS             complement(16570..17583)
                     /gene="tsaD"
                     /locus_tag="ACHJ1G_000546"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4762454.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17821..18036
                     /gene="rpsU"
                     /locus_tag="ACHJ1G_000547"
     CDS             17821..18036
                     /gene="rpsU"
                     /locus_tag="ACHJ1G_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4762455.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18147..19892
                     /gene="dnaG"
                     /locus_tag="ACHJ1G_000548"
     CDS             18147..19892
                     /gene="dnaG"
                     /locus_tag="ACHJ1G_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4762456.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            20087..21928
                     /gene="rpoD"
                     /locus_tag="ACHJ1G_000549"
     CDS             20087..21928
                     /gene="rpoD"
                     /locus_tag="ACHJ1G_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4762457.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22007..22513)
                     /gene="mug"
                     /locus_tag="ACHJ1G_000550"
     CDS             complement(22007..22513)
                     /gene="mug"
                     /locus_tag="ACHJ1G_000550"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4762458.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22638..22713
                     /locus_tag="ACHJ1G_000551"
     tRNA            22638..22713
                     /locus_tag="ACHJ1G_000551"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22671..22673,aa:Ile,seq:cat)
     gene            complement(22767..23531)
                     /gene="nfeF"
                     /locus_tag="ACHJ1G_000552"
                     /gene_synonym="yqjH"
     CDS             complement(22767..23531)
                     /gene="nfeF"
                     /locus_tag="ACHJ1G_000552"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4762459.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23808..24431
                     /gene="nfeR"
                     /locus_tag="ACHJ1G_000553"
                     /gene_synonym="yqjI"
     CDS             23808..24431
                     /gene="nfeR"
                     /locus_tag="ACHJ1G_000553"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4762460.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24585..26105)
                     /gene="aer"
                     /locus_tag="ACHJ1G_000554"
     CDS             complement(24585..26105)
                     /gene="aer"
                     /locus_tag="ACHJ1G_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4762461.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            26523..27902
                     /gene="ygjG"
                     /locus_tag="ACHJ1G_000555"
     CDS             26523..27902
                     /gene="ygjG"
                     /locus_tag="ACHJ1G_000555"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4762462.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(27944..28276)
                     /gene="ygjH"
                     /locus_tag="ACHJ1G_000556"
     CDS             complement(27944..28276)
                     /gene="ygjH"
                     /locus_tag="ACHJ1G_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4762463.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            28495..29478
                     /gene="ebgR"
                     /locus_tag="ACHJ1G_000557"
     CDS             28495..29478
                     /gene="ebgR"
                     /locus_tag="ACHJ1G_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4762464.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29662..32754
                     /gene="ebgA"
                     /locus_tag="ACHJ1G_000558"
     CDS             29662..32754
                     /gene="ebgA"
                     /locus_tag="ACHJ1G_000558"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4762465.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            32751..33200
                     /gene="ebgC"
                     /locus_tag="ACHJ1G_000559"
     CDS             32751..33200
                     /gene="ebgC"
                     /locus_tag="ACHJ1G_000559"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4762466.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            33263..34696
                     /gene="ygjI"
                     /locus_tag="ACHJ1G_000560"
     CDS             33263..34696
                     /gene="ygjI"
                     /locus_tag="ACHJ1G_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4762467.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            34830..35900
                     /gene="ygjJ"
                     /locus_tag="ACHJ1G_000561"
     CDS             34830..35900
                     /gene="ygjJ"
                     /locus_tag="ACHJ1G_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4762468.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            35917..38268
                     /gene="ygjK"
                     /locus_tag="ACHJ1G_000562"
                     /pseudo
     CDS             35917..38268
                     /gene="ygjK"
                     /locus_tag="ACHJ1G_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            38594..38887
                     /locus_tag="ACHJ1G_000563"
                     /pseudo
     CDS             38594..38887
                     /locus_tag="ACHJ1G_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            complement(39245..39886)
                     /locus_tag="ACHJ1G_000564"
     CDS             complement(39245..39886)
                     /locus_tag="ACHJ1G_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4762469.1"
                     /translation="MTNSYEEKTDVKREKLDRIISRSTINRFGERLKVAMKGMSNAEL
                     ARRSGMSETTIRKYLQGKIYPALDSLAIVADACGVSLTWLATGEQQANQTIVRDDAKQ
                     NYDNEYELLIHLLHKLKKEECVLLVDFISREGVNTLIRLVAMNASTISQDAVESIIDT
                     LPLRPVLKNAIKVGMAGSEDTDKEILRVIEKYNSSNNATQESRAVTVKKNVVG"
     gene            40038..40304
                     /locus_tag="ACHJ1G_000565"
     CDS             40038..40304
                     /locus_tag="ACHJ1G_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4762470.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            40315..42405
                     /locus_tag="ACHJ1G_000566"
     CDS             40315..42405
                     /locus_tag="ACHJ1G_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4762471.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQHPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            42477..43409
                     /locus_tag="ACHJ1G_000567"
     CDS             42477..43409
                     /locus_tag="ACHJ1G_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4762472.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            43412..43633
                     /locus_tag="ACHJ1G_000568"
     CDS             43412..43633
                     /locus_tag="ACHJ1G_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762473.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            43646..43900
                     /locus_tag="ACHJ1G_000569"
     CDS             43646..43900
                     /locus_tag="ACHJ1G_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762474.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            43902..44183
                     /locus_tag="ACHJ1G_000570"
     CDS             43902..44183
                     /locus_tag="ACHJ1G_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO4762475.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            44180..44452
                     /locus_tag="ACHJ1G_000571"
     CDS             44180..44452
                     /locus_tag="ACHJ1G_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762476.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            44457..44750
                     /locus_tag="ACHJ1G_000572"
     CDS             44457..44750
                     /locus_tag="ACHJ1G_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762477.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            44762..45292
                     /locus_tag="ACHJ1G_000573"
     CDS             44762..45292
                     /locus_tag="ACHJ1G_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4762478.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            45390..45932
                     /locus_tag="ACHJ1G_000574"
     CDS             45390..45932
                     /locus_tag="ACHJ1G_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762479.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            45936..46469
                     /locus_tag="ACHJ1G_000575"
     CDS             45936..46469
                     /locus_tag="ACHJ1G_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762480.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            46469..46984
                     /locus_tag="ACHJ1G_000576"
     CDS             46469..46984
                     /locus_tag="ACHJ1G_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762481.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            46988..47539
                     /locus_tag="ACHJ1G_000577"
     CDS             46988..47539
                     /locus_tag="ACHJ1G_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO4762482.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            47536..47847
                     /locus_tag="ACHJ1G_000578"
     CDS             47536..47847
                     /locus_tag="ACHJ1G_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762483.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            47862..48212
                     /locus_tag="ACHJ1G_000579"
     CDS             47862..48212
                     /locus_tag="ACHJ1G_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762484.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTL
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            48228..48560
                     /locus_tag="ACHJ1G_000580"
     CDS             48228..48560
                     /locus_tag="ACHJ1G_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762485.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            48553..48750
                     /locus_tag="ACHJ1G_000581"
     CDS             48553..48750
                     /locus_tag="ACHJ1G_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762486.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            48740..49036
                     /locus_tag="ACHJ1G_000582"
     CDS             48740..49036
                     /locus_tag="ACHJ1G_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762487.1"
                     /translation="MDSSSQLFWYLYITSFFGAFVITRWLCRKVISIFHKKYLIGQAA
                     TSLINALARYHKGECYSRIKTDDGFNIFVIPPEYRVQLDRENNTYHIRRSVDTA"
     gene            49033..49542
                     /locus_tag="ACHJ1G_000583"
     CDS             49033..49542
                     /locus_tag="ACHJ1G_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4762488.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSVLLAVTGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            49612..50037
                     /locus_tag="ACHJ1G_000584"
     CDS             49612..50037
                     /locus_tag="ACHJ1G_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4762489.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            50109..50609
                     /locus_tag="ACHJ1G_000585"
     CDS             50109..50609
                     /locus_tag="ACHJ1G_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4762490.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            50644..51072
                     /locus_tag="ACHJ1G_000586"
     CDS             50644..51072
                     /locus_tag="ACHJ1G_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762491.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            51056..51274
                     /locus_tag="ACHJ1G_000587"
     CDS             51056..51274
                     /locus_tag="ACHJ1G_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762492.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETRRQHEFTDMAGTPAVPAGKTK"
     gene            51284..51511
                     /locus_tag="ACHJ1G_000588"
     CDS             51284..51511
                     /locus_tag="ACHJ1G_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4762493.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRQAVDTPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARNVGQHR"
     gene            51492..51800
                     /locus_tag="ACHJ1G_000589"
     CDS             51492..51800
                     /locus_tag="ACHJ1G_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4762494.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            51797..52087
                     /locus_tag="ACHJ1G_000590"
     CDS             51797..52087
                     /locus_tag="ACHJ1G_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4762495.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            52090..52671
                     /locus_tag="ACHJ1G_000591"
     CDS             52090..52671
                     /locus_tag="ACHJ1G_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO4762496.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            52671..54335
                     /locus_tag="ACHJ1G_000592"
     CDS             52671..54335
                     /locus_tag="ACHJ1G_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762497.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            54335..55924
                     /locus_tag="ACHJ1G_000593"
     CDS             54335..55924
                     /locus_tag="ACHJ1G_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO4762498.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVSWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            55908..57239
                     /locus_tag="ACHJ1G_000594"
     CDS             55908..57239
                     /locus_tag="ACHJ1G_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO4762499.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            57361..57834
                     /locus_tag="ACHJ1G_000595"
     CDS             57361..57834
                     /locus_tag="ACHJ1G_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4762500.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            58011..59135
                     /locus_tag="ACHJ1G_000596"
     CDS             58011..59135
                     /locus_tag="ACHJ1G_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4762501.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            59135..60082
                     /locus_tag="ACHJ1G_000597"
     CDS             59135..60082
                     /locus_tag="ACHJ1G_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4762502.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            60126..60512
                     /locus_tag="ACHJ1G_000598"
     CDS             60126..60512
                     /locus_tag="ACHJ1G_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4762503.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAEAGQDAPRKKTGNKAEQARA"
     gene            60509..60928
                     /locus_tag="ACHJ1G_000599"
     CDS             60509..60928
                     /locus_tag="ACHJ1G_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4762504.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASV
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            60925..61485
                     /locus_tag="ACHJ1G_000600"
     CDS             60925..61485
                     /locus_tag="ACHJ1G_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO4762505.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            61486..61731
                     /locus_tag="ACHJ1G_000601"
     CDS             61486..61731
                     /locus_tag="ACHJ1G_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4762506.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            61728..63230
                     /locus_tag="ACHJ1G_000602"
     CDS             61728..63230
                     /locus_tag="ACHJ1G_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4762507.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            63239..63604
                     /locus_tag="ACHJ1G_000603"
     CDS             63239..63604
                     /locus_tag="ACHJ1G_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4762508.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            63619..64095
                     /locus_tag="ACHJ1G_000604"
     CDS             63619..64095
                     /locus_tag="ACHJ1G_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4762509.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            64222..>65748
                     /locus_tag="ACHJ1G_000605"
     CDS             64222..>65748
                     /locus_tag="ACHJ1G_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4762510.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     P"
BASE COUNT        15852 a        17271 c        17777 g        14848 t
ORIGIN      
        1 attattttcg ccaggctcta tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt
       61 ttaggtgggc aaaaagtggt gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa
      121 ataatcccca tcaatatgaa tactctgaag ctggaagtca gccgctcaac cattgatagc
      181 ctgattacga aagatcgtat gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa
      241 ccgtctgtag aaggcatcgc cactgcggcg cagacgttgg ggcaacgcac gctatcacca
      301 gaagacttac gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc aacagctgca
      361 caaatgacca tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg agtacaaaat
      421 actgtcgcag aagacctgtc gaaaaacggt cttgagctgg aaagcgtttc acttaccaac
      481 tttaaccaga cctcgaaaga acatttcaat ccgaacaatg cctttgacgc cgaaggttta
      541 accaaactga ctcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat
      601 gtagaagttg cggtgcgtga gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa
      661 caacaagaag cgtttatgac gcttgagcag gagcagcagg ttaaaacccg tactgccgaa
      721 cagaatgcac gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga gtagacgcga
      781 attctggctg aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca
      841 agaaaggttg aggctgaacg tgaagttcgt attaaagaga tcgaacaaca gcaggtcacc
      901 gaaatcgcca accagacgaa atcgatcgct attgccgcca aatcggaaca acagtcccag
      961 gcagaagcgc gtgctaacct tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa
     1021 accactcgcc agaccgcaga agccgatcgt gctaaacaag ttgccctaat cgctgccgcg
     1081 caggatgcag aaaccaaagc ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc
     1141 gcagagatgc aggcggcggc tatcgttgag ttagccgaag ctacacgtaa aaagggtctg
     1201 gcggaagcag aagcacaacg tgcgctgaac gatgctatca acgtactttc tgatgaacaa
     1261 accagcctta aattcaaact ggccttgttg caggcgctac ctgcggtaat agaaaaatcc
     1321 gttgagccga tgaaatctat tgacggtatc aagattattc aggtcgatgg tctgaatcgt
     1381 ggcagcgctg cgggtgatgc aaacacgggt aatgtggggg gcggaaacct ggcggaacaa
     1441 gcattatcag ccgctctctc ttaccgcaca caggcaccgc tgattgactc cttgctcaat
     1501 gaaattggcg tttcaggcgg ctcactggcg gcattgactt cacccttaac ctcaacaact
     1561 cccgtcgccg aaaacgtaga ataaattttc agctatgaac gtccctgtca caaaaagccg
     1621 gggatttttt atatctgcgt tccgctaaaa actgcaaatg atcaggtcgt tgcaacgttt
     1681 tgcgtgaccg ctcggggaag gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg
     1741 ctcatcaaca atcggggagt cagcaaaggg ctgaaacggg aaagcccctc cctaggaagg
     1801 ggccttacag aaggaaaggg ttatgatgaa gctcgtcatc atactgattg tgttgttaat
     1861 cgtaagtttc gcagcttatt aacagccaat cagagggggg agaaatcctc cctcaccctt
     1921 gttcctttac tttaggttga aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc
     1981 ctggcccaaa aagccgggga ttttttatat ctgcgttccg ctaaaaggtg caaatgctca
     2041 ggacgttgca gcgttttgcg tgaccgctcg gggaaggcaa aactgcttca gggaaagcgt
     2101 tgcgcggggt ccggcgctca tcaacaatcg gggagtcagc aaggggctga aacgggaaag
     2161 cccctcccga ggaaggggcc ttatagaagg aaagggttat gatgaagttc gtcatcatac
     2221 tggttgtgtt gttactgtta agtttcccga cttactaaca actgatcaga ggggggagaa
     2281 atcctccctt acccttgttc ctttacttta ggttgaaaaa acaacagcgt caataggcct
     2341 gcctgctacg aagcgagatc tgtgaaccgc tttccggtta gcctttttta tcctgttgga
     2401 tcttcttgat gatgttagtg gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc
     2461 cgccgttggc ccagacttct ttactcccgg cgatctcttc tggtttatag tcgccgccct
     2521 tcaccagtaa atctggcagg atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg
     2581 acaccaccca gtcgaccgct tccagtgcgc ccagcacaat catacgctgt tcgagcgggt
     2641 taacggggcg ggaatcccct ttcagccgtt tggtggaggc atcgctgttc acagcgacaa
     2701 tcagacggtc acccagtttg cgagcatttg ccagatagga gacgtgcccg gcgtgcagga
     2761 tgtcaaagac accgttggtc attaccacct tttcgccgcg tttacgcgct gccgctacag
     2821 caagcttcag ttcctcttcg gtcatcacac caaagccggt atctgcgcgc ccacgcactg
     2881 cattttccag ctcgatcggc gaaaccgtgg aggttcccag tttgccgacc accacgccag
     2941 ccgccgcatt ggcaaagaag caggcttctt ccagcgaatt acccgctgcc agcgttgccg
     3001 ccaggacgcc aatcaccgtg tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg
     3061 ttggcatatg cagcggcgct ttacccggtt gcagcagcga cataccctgt tcggaacggg
     3121 tcactaacag agccgagagt tcgtaatcgg caatcagttt catgccgcgc tcaacaatct
     3181 cttcttcggt cttacattta ccgacaacag cttcaaattc cgagagattc ggtgtcagca
     3241 gcgtagcgcc gcggtagcgc tcaaaatcgg taccttttgg atcaatcagc accggaacgc
     3301 ccgctttacg cgccagttgg atcatctgct gtacgcttgc cagcgcacct ttggcgtagt
     3361 cagaaagcac cagcgcgcca atcgaactca gcgcctgatt aatccgctcg tgcagcggct
     3421 gcggatcaac accttcgaaa ccttcttcaa aatccaggcg gatcagctgt tggttgcggg
     3481 aaagtacccg taatttggtg atagtcggat gcgtcggtac agaaacgaag tcgcatttga
     3541 cgttgacgtc ggccagagat ttactcagcg cacgcgccgc atcgtcaatg cccgtcagcc
     3601 cgaccaggcg agcattggca ccgagagaag tgatgttcat tgccacgtta gccgcgccgc
     3661 ccggacgttc ttcgatggta ttgactttaa ccacgggcac cggcgcttcc ggcgagatac
     3721 ggctggtggg accgtaccag taacgatcca gcatcacatc accaaccacc atcactcctg
     3781 cacgttcaaa ctctggcagc gttactttca ttcctgtctc ctgagagatt caaaatttgc
     3841 gcgcgataat accatacttc attcttccac cagccacttc tgccagcttg cccgcaccag
     3901 ttcacgctct gcggtgaagc aatcctccgg cacatggccc ggcaattcct gtaatgccag
     3961 atgatgaagt tcatcgcgca gcgtagtata cgcacgggtc agcgccatcg cttcctgctc
     4021 ttccataatg tcgttttgcg ccagtagttc cagaatgcgc acgttgtctg accagcgcgt
     4081 taacttcggt ttttcatgag cgtagcgcaa caccagatat tgggtaataa attcgatatc
     4141 ggtaattccg ccttcatcag ctttgatatc aaagcgatcg cgatgtttat tgccgagatg
     4201 agcgcgcatt ttctcgcgca tttcccgcac ttccgtttgc agagttttac cttcacgcgg
     4261 cagcgtcata atctcgcgac gcaccgcgtc aaagtgcgcg gtgagctgcg gatcgccgta
     4321 cactacccgc gcacgcacca gcgcctgatg ttcccacgtc caggcctcgt ttttctgata
     4381 atcggcaaat gcttctgcgg atgtcaccag cattcccgcg gccccggacg ggcgtagccg
     4441 tgcatccact tcatacaaaa tgccggaaga ggtacgcgta ctgaacagat gcataatgcg
     4501 ttgcgccaga cgcagataaa actgtcgccc gtcgatttcc cgctcgccgt cagtcatcgc
     4561 atccattggg caatcatgga ggaagatcag gtcaagatcg gaactgtagc ctaactccca
     4621 gccgcccagc ttgccgtagc cgaccaccgc aaaaccacgg ccttcgcgat cgttgaggtg
     4681 atttggctta ccgtagcggg ccaccatttg aacccacgcc tgctgaacga cggcatctat
     4741 catagcctct gccagccagg ttaagtgatc gctcactttc atcaccggta gcgtaccagc
     4801 gatatccgct gcggcgatgc gtaacagctg tgcctgtttg aactgacgca gcgcctcaag
     4861 ctgttgctct tcgtcatctt ccggcacgcg cagcaaatac tggcgcaact catcgcggta
     4921 ggcatcggtc gccgtcggct ggtaaagggt gtttggatcg agcaattcat ccagcaataa
     4981 tggataacgc gccagttggc tggcaatcat cggcgacgcg gcacacagag aaatcaaatg
     5041 tttaagcgcc gcggggaatt cactgagcaa ttctaaatag gtggtgcggg taacaatccc
     5101 caccagcaag gcggtaatgc gcgacagcgt aacggcagcg tcttcacgcg cacagacatc
     5161 acttagcaga tgcggcatca gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg
     5221 cttatccagc tctttgcgga aatcggcaat cagcgttagc acctgtttgc gatcatcctc
     5281 gctaagatgc gccagcactg gcgtagtgtc atcttcctgc aacgcatcct gccacagctc
     5341 acgccactgt tccgacagcg actcttcctg agtttcactt tcgtcgtcgc caatcaattc
     5401 attaaacacc cggcgcacat tggtcatatg tgcggtcagc gccccggtca gttgcggcca
     5461 gtcagcaaag tccatcgccc acgccagccg cgcacggtta agctcatcag aaggaagcgt
     5521 ctgggtttgt tcgtcgttaa tgctttgcag caggttttcc agacgccgca ggaacagata
     5581 cgccactcgc aattgttcag catcgttttc agaaagcaga tgcagcgcgg caatgacgct
     5641 gagcgttggc agtaaagagc gcgactgcag cgacggttcg cgcccaccgc gaatgagctg
     5701 gaacacctga acgataaatt caatttcgcg aatgccgcct gcgccgagtt tgatattgtc
     5761 ggtcaaacca cgtcgacgca cttcacgggc aatcatccct ttcatgttgc gcagcgactg
     5821 aatcacgctg aaatcgatgt aacgacggaa aacaaacggg cgcagcatcg cacgcaactc
     5881 gttagcatag acgccttcgc tatcgcccat aatccgcgcc ttgaccatcg cgtaacgctc
     5941 ccagtcgcgc ccctgctcct ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg
     6001 gccactttcg ccaaacggac gcagccgcat atccacgcga tagacgaagc catcctgcgt
     6061 tggttgatcc aacactttaa tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc
     6121 cagttcccgg cgtccgccct gcgtacaacc atgttccggc caggcaaaaa tcagatcgat
     6181 atctgaggag aaattcagtt ccccaccgcc cagcttaccc atgcctaaaa tcagcagcgg
     6241 ttgcgcttcg ccctgcgcat tgcacggcgt tccccactcg cggcagcaag cgtcatacag
     6301 ccagtcacgc gcggcgacaa tcagcgtctc cgccagatgg ctgagctgct gcaatatgct
     6361 ctcttcagta accaaagcca gcgtttgcgc ccaggcgatg cgcaccataa tgcgccgccg
     6421 gaatagccgc agctcgcgca ttaacccggc ttcgtcactc acattactga gcgcctcctg
     6481 caaccatgcc gcgtaatgct gccattcgtc ggcctgcggc ggttggcttt ccagttccgt
     6541 cagccactct ggatgcacaa tcacgctgtc ctgcacaaaa tcactaaaag taagtactga
     6601 cttcgcctgt gcgctaagtg attcctcggc taaaggctct ggcagccgct caacaacggt
     6661 ctgccagtac tgctgtaacg gtgaagagag cggcttcata agcgatttta tccttgcctg
     6721 attaacgttt tccgctgtgc aaccagaacg gttcctgatt gtttgcctca ttacggaaat
     6781 gttcaatttc aatgcgttgc ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca
     6841 gccaggcttg cgcgacgaca ggatcatagt aacctgccag cagcagtatt gagtcaatat
     6901 cacgcgtcag gcgtggcaac tgatcatggt agcgatcgcc taacggctgg cagaaaacgc
     6961 ttttcagttc agcggcatgg cgggaaagat ggatatcggc aaagcgtttg aaggaatcgc
     7021 tgattttacc ctgcgctttg gcatctaaaa acggctgcca ggctttgctt accaaccatt
     7081 cggtcaacgc cagctttgcc attgccgttt cggtagagta gaccgccgtc acggcagaaa
     7141 ccgcagaagc gatggtcgct tcgcattgag tcagcaaatc acgtaagtga gtgctcgctt
     7201 tacgcggcac aatgccaccg aacagcatca gtgcatggcg aacaaggcca atggctgcca
     7261 gcacctgctc tttcgccgca tcgttgccgc gtacccacag ttcttcatga tactgccatt
     7321 gcgctaacgc cagctcgaac gccgcttcca gcccctgttc cacatcggct tttgccgcaa
     7381 catgcaaaat ggtggtcggt ttgatttcac gcgccggatt gccctgcgcc agatgatagc
     7441 cacgcgccgc tttgctcagg ctgccctggc gtaatccggt ttgcgatacc agttggttcg
     7501 ccagtttcag caccgcgcgc gtgtcgccgc taagcagttc cagttccagc tcacagatag
     7561 gttcagcaaa ttcccccgct ttcacctccc cctgatcgag agcgatttca attcggctgt
     7621 catcgaccgc caccagccat ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc
     7681 gggaagcgag atcggcgggc aattcgccgt tcggccagac ttccgtcggt aactgcgcca
     7741 ggtcgagcgt cggttcgctc aacgccacgt tatattccgg gcgctggtgt aagccgcctg
     7801 tcactcttcc tgcaactttc atggtcatct catagcgacc gttttcgcca cgaatacgta
     7861 agcccatatc gtgcccacgc agccagttat ccggcgtttc gtagtaaata ttcagcaact
     7921 gcacggggtc atggtgctcg ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg
     7981 cactgtgatt aacaataaac tttaattcga tttcctgagc catggccttt acttatcggt
     8041 tatgtcacat aagtgacgat gaacggcgaa cttaatgcga tcttttttgt cagtagatag
     8101 tattttgcgc caaattgcca tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt
     8161 ggcaagacct gacacagagg attccgattg atgtcgaatg ctttgcgtag cgacactgat
     8221 gccactacta tcgttccact ttccatgaca ataacgacag cctgatgcca aaattacgcc
     8281 tgatcggatt aactttactc gcacttagcg ccactgccgt ctcacacgct gaagaaacgc
     8341 gctatgtttc tgatgaactg aatacctggg tccgtagcgg tccgggagat cattatcgcc
     8401 tcgtgggcac ggttaacgcc ggcgaggaag tgaccttatt acaaactgac gccaacacca
     8461 attatgccca ggtgaaagac agctctggcc gtaccgcctg gatcccgttg aaacaactta
     8521 gcactgagcc aagcctgcgc tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga
     8581 ccgataaact caccaatatc gataacacct ggaatcagcg caccgcagaa atgcagcaaa
     8641 aagtggcgca gagcgacagc gtgatcaacg gattaaaaga agaaaatcag aaattgaaaa
     8701 acgagctgat tgtcgcgcag aaaaaggtcg atgccgccag cgtacagttg gatgacaaac
     8761 agcgcaccat catcatgcaa tggtttatgt atggcggtgg cgtgctgggg cttggcttgc
     8821 tgctcggtct ggtactgcca cacctgatcc caacgcgcaa acgcaaagat cgctggatga
     8881 actaaatcgc cttcttcgtt gcactgtctt attattagga attgtgtatt tgacgaaggg
     8941 atgatggcgt gaagatttat ctggtcggtg gtgctgttcg ggatgcattg ttagggctac
     9001 cggtcaaaga cagagattgg gtggtggtcg gcagtacgcc acaggagatg ctcgacgcgg
     9061 gctaccagca ggtaggccgc gattttcctg tgtttctgca tccgctaacg catgaagagt
     9121 atgcgctggc acgtaccgaa cggaaatccg gttccggtta caccggtttt acttgctatg
     9181 ccgcaccgga tgtcacgctg gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc
     9241 tggctcagga cgataacggc gagattatcg acccgtacaa cggtctgggc gatctgcaaa
     9301 atcgtctgtt gcgccatgtt tcccccgctt ttggcgaaga tccgttacgc gttctgcgcg
     9361 tggcgcgttt tgctgcgcgt tatgcccacc tcggttttcg tattgccgat gaaactctga
     9421 cgctgatgcg cgagatgacc catgcgggtg aactggaaca cctgacgcct gaacgggtat
     9481 ggaaagagac ggaaagcgcc ctcaccaccc gcaatcccca ggtgttcttc caggtactgc
     9541 gcgattgcgg tgcacttcgc gttttattcc cggaaattga cgccctgttt ggcgttccgg
     9601 cccctgccag gtggcatccg gaaatcgaca cgggtattca taccttaatg acgctctcaa
     9661 tggcggcgat gctgagtccg caggttgatg tccgtttcgc aactttatgc cacgatctcg
     9721 gtaaaggact gacgccgcca gaactttggc cgcgtcatca tgggcatggc ccggcgggtg
     9781 ttaagttagt ggaacaacta tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag
     9841 ccagactggt ggctgagttt cacgatctca tccacacctt cccaatgctg aacccaaaaa
     9901 ccatcgtcaa attatttgat tccatcgatg cctggcgtaa accgcagcgt gtcgagcaac
     9961 tggcgctgac cagcgaggct gacgtacgcg gcagaaccgg ttttgaatca gcggactacc
    10021 cgcaaggccg ttggttgcgc gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg
    10081 tcgttgaagc gggatttaaa ggtgtggaga ttcgcgagga gctgacccga cggcggattg
    10141 cggcggtagc cagttggaag gaacaacgtt gcccaaagcc tgactgagta taaaactaaa
    10201 cgttaacacg aaccatcccc tcgccccttc ggggagaggg ttagggtgag gggaacagac
    10261 cagcaccggt gcgaacaatc acccccaccc cggccctctc cctacagagc gagggagaaa
    10321 accaggcctg ctcccgaacc ttttctcaat caaaagaaca cgacgtacac cgcagccgcc
    10381 acaataaagc gataaatggc gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag
    10441 gttttaatcg ctatcagcgc caccacaaaa gcggtgataa acccaacggc aaacatcggg
    10501 atatcgccgg ttgtcaggaa gccccagctt ttgtagagat cgagcgccgt tgcgcccatc
    10561 atcatcggta ccgccagcag gaacgagaac tcggaagcag cgtaacggct cacgcccatc
    10621 agcatcccac ctgaaatggt ggccccggaa cgggagaaac ccggccacag cgccagacac
    10681 tggaaacagc caatcataaa cgcctgacga taggtcatat catcaagacc cggcgcacgc
    10741 ggctctttcg gcttcaggca ttcggcggca atcagcaaca aaccgccaac gaccagtgca
    10801 tacatcacat ttatcgggtt aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt
    10861 actaccgccg gaatcatccc cagcaaaatg tggatcagcg ttaaacgacc tttgctttct
    10921 ccttcgtgct gcaacgggcg gccaaagtgg atgccaatca ggccaaacag acgccgccag
    10981 aacatcacta ctaccgccag aattgatcct aactggatca caacttcaaa ggttttcgcc
    11041 gtgtcgccct cgaaccccaa caagtgaccg acaataatca tatggcccgt gctggatacc
    11101 ggcagaaatt ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag cagcgagtgc
    11161 atatcgctca tcaataaacc cctaaattat taaaatgtac cgcttgtccg aactactgcg
    11221 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    11281 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    11341 tgagtttgat acgcacccac ggcgagttaa agcgtgctaa cagcagctcc gccacctctt
    11401 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    11461 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    11521 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    11581 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    11641 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11701 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11761 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11821 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11881 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgccc
    11941 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    12001 gcctgtcttg gacacatctg gcccgttttc ttcggattta aaggaggaaa aggcgttgct
    12061 accgcttttg gtgccatcgc acccattggc tgggatctca ccggagtaat ggcgggaacc
    12121 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    12181 attgctccgt tttatgtatg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    12241 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    12301 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    12361 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    12421 cagaaattcc acgcagacac gtaatttcat gctgcgatag agcggctccc ggtaaacagc
    12481 ccagatattg gcgctttgtg catactccgg caatacttgc accagtttgc cactctccag
    12541 aaacggcaac acatcccact cggaacgcag cataatccct ttgccctcca gcgcccattg
    12601 caacactatc tcgccgctat tggaggaaag atgcccgcta accttcaccg attttttctc
    12661 ctgaccgttc cccaactccc atattccgtg ggtcatatcg cgctctttgg tcaccagaca
    12721 gtcatgacga cttaattcct gtaatgattg tggctgcgga tatttttgca gatactcagg
    12781 tgctgcgcat aatattcttt tatttttcgt taacagatgc gcaatataat aatcaggaat
    12841 ttcgtcatta atacgaatat ccagatcaat attatcctgc actaaatcaa tttgccgatc
    12901 gaacaattca aaatgcacct gtaactcagg ataattgcgc ataagttcgg taatggctgg
    12961 cgcaatatgg ctgcgcccaa aaccaaaact gcaaccaata cgaatcatcc cctccgggcg
    13021 cgttttgatt tgcgtgacgt catcgaccag tcgctgatat tgggtgagga tttccagcgc
    13081 atgttcgtaa caacgttggc cactttcagt cagcgccacg ccgcgagcgg agcggttcag
    13141 cagcgttgtt gccagcgtat tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt
    13201 ctgcccaagc gtcgccgctg cagcactaaa actcccgctg tggacgattt ctaccagtac
    13261 ctgcaagtct ttggctaaag gccagctatt cagcatcgtt aattatccgc agttgtgata
    13321 agcgcagtgt atttcgcaaa acattgccat tccctgctct ggcgcaatat tcattcttat
    13381 tttcgcgttg attcacgaac tgttaattac atattcacgg tggcaaaaaa tataaaacca
    13441 catttttagt ggtagtttgt ggcggtgaat ttttccagac aaatacaaaa actggagttg
    13501 ccatgatgag cgaaagtaat aagcaacagg cagtgaataa gttgacagag attgtcgcta
    13561 actttaccgc catgatttct acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc
    13621 taaaggatgc cgaaacgtcg tcgatgggga aaattatcta ccatacgatg ttcgacaaca
    13681 tgcaaaaagc gattgacctg aatcgtcctg cctgtcagga caccggggag attatgttct
    13741 tcgttaaagt cggttcccgc ttcccactgc ttggcgagct gcaaagcata ctcaaacaag
    13801 ccgtggaaga ggcaaccgtc aaagcgccac tacgtcacaa tgcggtagaa atttttgacg
    13861 aagtaaacac cggcaaaaat accggtagcg gcgtaccgtg ggtcacctgg gacatcatcc
    13921 ccgacaatga cgatgcggaa atcgaagttt acatggcagg cggcggctgc acgctacctg
    13981 gccgctcgaa agtgttaatg ccgtcagaag gctacgaagg cgtggtgaaa ttcgtcttcg
    14041 aaaatatctc caccctcgcc gtaaacgcct gtccaccggt actggtgggc gtgggcatcg
    14101 ccacctcggt ggaaaccgcc gccgtgctct cgcgaaaagc cattttgcgc ccgattggca
    14161 gccgccatcc caatccaaaa gcggcagagc tggagctacg cctggaagaa ggactcaacc
    14221 gtctgggaat tggcccacaa gggctgactg gcaacagttc agtgatgggc gtgcatatcg
    14281 aatctgccgc ccgccatccg tcaaccatcg gcgttgctgt ttctacaggc tgctgggcgc
    14341 atcgtcgcgg cacactgctg gttcatgccg atctcacctt tgaaaatctg tctcacaccc
    14401 ggagcgcgtt atgaaaaaga tcctgacaac cccgatcaaa gctgaagatc tgcaagatat
    14461 tcgcgtcggc gatgtgatct acctgaccgg tacgctggtg acctgccgtg acgtttgtca
    14521 ccgccgcttg atcgaactga aacgtccgat cccttacgat ctcaacggca aagcgatttt
    14581 ccacgctggc cccatcgtgc gcaaaaacgg cgataaatgg gagatggttt ccgttggccc
    14641 gacaaccagt atgcgtatgg aaagttttga acgtgaattt atcgagcaga ccggcgtgaa
    14701 actggtggtt ggcaaaggtg gtatggggcc gctgaccgaa gaaggctgcc agaaattcaa
    14761 ggcgctacat gtgattttcc cggcaggctg cgcggtgctg gcggcaaccc aggtagaaga
    14821 gattgaagaa gtgcactgga cagaactcgg gatgccggag tcactgtggg tctgccgggt
    14881 caaagagttc ggcccgctga ttgtctctat tgatacccac ggcaacaacc tgatagccga
    14941 aaacaaaaag ctgttcgccg aacgccgcga tcccatcgtg gaagagatct gcgagcacgt
    15001 ccattacatc aaataaccct cccggagagg ctcacccctc tcctttttcg caggcataac
    15061 acgatgaaac cttccactga atggtggcga tatcttgcgc cgctggcggt catcgccatt
    15121 attgctctac ttccggttcc cgcagggctg gagaaccata cctggctcta ctttgccgtt
    15181 tttactggcg tgatcgttgg actgatcctc gaacccgtgc cgggtgccgt ggtggcgatg
    15241 gtgggtatct ccatcatcgc catactctct ccctggctgc tgttcagccc cgaacagctc
    15301 gcccagccag gctttaaatt cactgcaaaa tccctctcgt gggcggtttc cggtttttct
    15361 aattcggtta tctggctgat tttcgccgcc tttatgtttg gcacaggcta tgaaaaaacc
    15421 gggcttggac gccgcattgc gctgtttctg gtgaaaaaga tgggacatcg cacgctgttt
    15481 ctcggctatg cggtgatgtt ctccgagctg atcctcgcac ctgtaacacc gtccaactcg
    15541 gcgcgtggtg cggggattat ctatcccatc atccgtaacc tgccaccgct ctatcaatca
    15601 caaccaaacg acagcagttc gcgcagcatt ggctcgtaca tcatgtggat ggggattgtt
    15661 gccgactgcg tgaccagcgc cattttcttg acggcgatgg caccaaactt gctgttaatt
    15721 ggactgatga aaagcgcatc tcacgccacg ctgagttggg gcgactggtt cctcgggatg
    15781 ttgccgctca gtattttgct ggtgctgctg gttccctggc tggcttatgt gctgtacccg
    15841 ccggtactga agtctggcga tcaggtgccg cgctgggcag agacggaact gcaggcaatg
    15901 ggcccgctct gttcgcgtga aaaacggatg ctggggctga tggtaggcgc gctggtgctg
    15961 tggattttcg gcggtgatta tatcgatgcc gcgatggtcg gttacagcgt ggtggcactg
    16021 atgctgcttc tgcgcattat cagttgggac gacattgtca gtaataaagc ggcgtggaac
    16081 gttttcttct ggctggcctc gcttatcacc ctcgctaccg gactcaacaa cactggtttt
    16141 attagctggt ttggcaaact gttagcaggc agcttaagcg gttattcgcc aacgatggtg
    16201 atggtggcgt tgattgtggt gttttatctg ctgcgctact ttttcgccag cgccacggcg
    16261 tatacctccg ccctcgcacc gatgatgatt gccgctgcgc tggcgatgcc ggaaatcccg
    16321 ctgccggtgt tctgcctgat ggttggcgcg gcaattggtc tggggagcat tcttacacca
    16381 tacgccaccg gacccagtcc gatttactac ggtagtggtt atctgccaac ggtggattac
    16441 tggcgactgg gggcgatttt tggactgata ttcctcgtgt tgctggtgat taccggcttg
    16501 ctgtggatgc ccgtggtgtt gctttaagtg aggacggtgc cggaaattgc tctccggcac
    16561 cggatagttt tacgcagccg gtaactctgc cagtggccag cgcggacgca cgctaacgcc
    16621 gagatccgcc gtcgcgcctg ctttaaaccg caccattccg gcataggcga tcatcgcgcc
    16681 gttatcagta caaaattccg gacgcgcgta gaacacttcg ccgcggcgtt ttttcatcat
    16741 ttccgccagc ttcgcccgta acgtgcggtt agcactcacg ccgcccgcca tgaccagtcg
    16801 cttaaagccc gtctgatcca acgctcgctt acacttaatc atcaacgtat cgaccaccgc
    16861 atcttcaaag gcgcgggcga tatcagcacg cgtctggtcg tcggtgccgt tgtcacgaat
    16921 cgtgttcgcc gcaaaggttt tcagaccaga aaagctgaaa tccagccccg gacggtcggt
    16981 catcggacgc gggaaaacaa agcgcccggc agtaccctga gccgccattt tcgacagtaa
    17041 cggtccgcca ggataatcca gccccagcag cttcgcggtt ttatcaaacg cttcaccggc
    17101 ggcatcatcg atagactcgc cgagcagctc gtactgacca atgccagtca cgctgattaa
    17161 ctgcgtatga ccgccggaca ccagcagcgc gacaaacgga aattccggcg ggttatcttc
    17221 cagcatcggc gctaacagat gcccttccat atggtgtacc gggattgccg gaacgttcca
    17281 ggcaaacgcc agagaacgcc ccacggttgc gccaaccagc agtgcgccga ctaatccagg
    17341 gcctgcggta taggccacag catcaatgtc ttttgccgtt aaaccagact cctttagcgc
    17401 cgcctggatc aacggtacgg ttttgcgcac gtgatcgcgg gaggccagtt caggcacgac
    17461 gccgccgtag tcagcgtgca atttcacctg actatacaat tggttggcta acaaaccttt
    17521 ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa gtttcaatac ccagtacacg
    17581 catgacttgt tttacctcgc tttattaccg cgcagtgtag gaccaatgcg ggttgatgta
    17641 aaactttgtt cgcccctgga gaaagcctcg tgtatactcc tcacccttat aaaagtccct
    17701 ttcaaaaaag gccgcggtgc tttacaaagc agcagcaatt gcagtaaaat tccgcaccat
    17761 tttgaaataa gctggcgttg atgccagcgg caaaccgaat taatcaaagg tgagaggcac
    17821 atgccggtaa ttaaagtacg tgaaaacgag ccgttcgacg tagctctgcg tcgcttcaag
    17881 cgttcctgcg aaaaagcagg tgttctggcg gaagttcgtc gtcgtgagtt ctatgaaaaa
    17941 ccgactaccg aacgtaagcg cgctaaagct tctgcagtga aacgtcacgc gaagaaactg
    18001 gctcgcgaaa acgcacgccg cactcgtctg tactaattcc ccgagagcgt tgctctccga
    18061 tcagaccgag ttgtagttgt aaggccgtgc ttccgaaagg aatgcgcggc ttattttcgt
    18121 ttatgaattg ctaaaaatcg gggcctatgg ctggacgaat cccacgcgta ttcattaatg
    18181 atctgctggc acgcactgac atcgtcgatc tgatcgatgc ccgtgtgaag ctgaaaaagc
    18241 agggcaagaa tttccacgcg tgttgtccat tccacaacga gaaaaccccg tcattcaccg
    18301 ttaacggtga gaaacagttt taccactgct ttggatgtgg cgcgcacggc aacgcgatcg
    18361 acttcctgat gaactacgac aagctcgagt tcgttgaaac ggtcgaagag ctggcagcaa
    18421 tgcacaatct tgaagtgcca tttgaagcag gcagcggccc cagccagatc gagcgccatc
    18481 agaggcaaac gctttatcag ttgatggacg gtctgaatac gttttaccaa caatctttac
    18541 aacaacctgt tgccacgtct gcgcgccagt atctggaaaa acgcggatta agccacgagg
    18601 ttatcgctcg ctttgcgatt ggttttgcgc cccccggctg ggacaacgtc ctgaagcggt
    18661 ttggcggcaa tccagaaaat cgccagtcat tgattgatgc ggggatgttg gtcactaacg
    18721 atcagggacg cagttacgat cgtttccgcg agcgggtgat gttccccatt cgcgataaac
    18781 gcggtcgggt gattggtttt ggcgggcgcg tgctgggcaa cgataccccc aaatacctga
    18841 actcgccgga aacagacatt ttccataaag gccgccagct ttacggtctt tatgaagcgc
    18901 agcaggataa cgctgaaccc aatcgtctgc ttgtggtcga aggctatatg gacgtggtgg
    18961 cgctggcgca atacggcatt aattacgccg ttgcgtcgtt aggtacgtca accaccgccg
    19021 atcacataca actgttgttc cgcgcgacca acaatgtcat ttgctgttat gacggcgacc
    19081 gtgcaggccg cgatgccgca tggcgagcgc tggaaacggc gctgccttac atgacagacg
    19141 gccgtcagct acgctttatg tttttgccgg atggcgaaga ccctgacacg ctggtacgaa
    19201 aagaaggtaa agaagcgttt gaagcgcgga tggagcaggc gatgccactc tccgcatttc
    19261 tgtttaacag cctgatgccg caagttgatc tgagtacccc tgacgggcgc gcacgtttga
    19321 gtacgctggc actgccattg atatcgcaag tgccgggcga aacgctgcga atatatcttc
    19381 gtcaggaatt aggcaacaaa ttaggcatac ttgatgacag ccagcttgaa cgattaatgc
    19441 caaaagcggc agagagcggc gtttctcgcc ctgttccgca gctaaaacgc acgaccatgc
    19501 gtatacttat agggttgctg gtgcaaaatc cagaattagc gacgttggtc ccgccgcttg
    19561 agaatctgga tgaaaataag ctccctggac ttggcttatt cagagaactg gtcaacactt
    19621 gtctctccca gccaggtctg accaccgggc aacttttaga gcactatcgt ggtacaaata
    19681 atgctgccac ccttgaaaaa ctgtcgatgt gggacgatat agcagataag aatattgctg
    19741 agcaaacctt caccgactca ctcaaccata tgtttgattc gctgcttgaa ctgcgccagg
    19801 aagagttaat cgctcgtgag cgcacgcatg gtttaagcaa cgaagaacgc ctggagctct
    19861 ggacattaaa cctggagctg gcgaaaaagt gatttaacgg cttaagtgcc gaagagcgat
    19921 cgggaagccc ccgacagccg cactgagagg cagcggcaaa tatataagta cgccctcgta
    19981 attatcgttg gcggtaaaca accgttggat ttcagcgtta acggctgaag gacatcgggt
    20041 caatcgccca acaccaacct catgaaataa gtgtggatac cgtcttatgg agcaaaaccc
    20101 gcagtcacag ctgaaacttc ttgtcacccg tggtaaggag caaggctatc tgacctatgc
    20161 cgaggtcaat gaccatctgc cggaagatat cgtcgattca gatcagatcg aagacatcat
    20221 ccaaatgatc aacgacatgg gcattcaggt gatggaagaa gcaccggatg ccgatgatct
    20281 gatgctggct gaaaacaccg cggacgaaga tgctgccgaa gccgccgcgc aggtgctttc
    20341 cagcgtggaa tctgaaatcg ggcgcacgac tgacccggta cgcatgtaca tgcgtgaaat
    20401 gggcaccgtt gaactgttga cccgcgaagg cgaaattgac atcgctaagc gtattgaaga
    20461 cgggatcaac caggttcaat gctccgttgc tgaatatccg gaagcgatca cctatctgct
    20521 ggaacagtac gatcgtgttg aagcagaaga agcgcgtctg tccgatctga tcaccggctt
    20581 tgttgacccg aacgcagaag aagatctggc acctaccgcc actcacgtcg gttctgagct
    20641 ttcccaggaa gatctggacg atgacgaaga tgaagacgaa gaagatggcg atgacgacag
    20701 cgccgatgat gacaacagca tcgacccgga actggctcgc gaaaaatttg cggaactacg
    20761 cgctcagtac gttgtaacgc gtgacaccat caaagcgaaa ggtcgcagtc acgctgccgc
    20821 tcaggaagag atcctgaaac tgtctgaagt attcaaacag ttccgcctgg tgccgaagca
    20881 gtttgactac ctggtcaaca gcatgcgcgt catgatggac cgcgttcgta cgcaagaacg
    20941 tctgatcatg aagctctgcg ttgagcagtg caaaatgccg aagaaaaact tcattaccct
    21001 gtttaccggc aacgaaacca gcgatacctg gttcaacgcg gcaattgcga tgaacaagcc
    21061 gtggtcggaa aaactgcacg atgtctctga agaagtgcat cgcgccctgc agaaactgca
    21121 gcagattgaa gaagaaaccg gcctgaccat cgagcaggtt aaagatatca accgtcgtat
    21181 gtccatcggt gaagcgaaag cccgccgtgc gaagaaagag atggttgaag cgaacttacg
    21241 tctggttatt tctatcgcca agaaatacac caaccgtggc ttgcagttcc ttgacctgat
    21301 tcaggaaggc aacatcggtc tgatgaaagc ggttgataaa ttcgaatacc gccgtggtta
    21361 caagttctcc acctacgcaa cctggtggat ccgtcaggcg atcacccgtt ctatcgcgga
    21421 tcaggcgcgc accatccgta ttccggtgca tatgattgag actatcaaca aactcaaccg
    21481 tatttctcgc cagatgctgc aagagatggg ccgtgaaccg acgccggaag aactggctga
    21541 acgtatgctg atgccggaag acaagatccg caaagtgctg aagatcgcca aagagccaat
    21601 ctccatggaa acgccgatcg gtgatgatga agattcgcat ctgggggatt tcatcgagga
    21661 taccaccctc gagctgccgc tggattctgc gaccaccgaa agcctgcgtg cggcaacgca
    21721 cgacgtgctg gctggcctga ccgcgcgtga agcgaaagtt ctgcgtatgc gtttcggtat
    21781 cgatatgaac accgaccaca cgctggaaga agtgggtaaa cagttcgacg ttacccgcga
    21841 acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg cgtcacccga gccgttctga
    21901 agtgttgcgt agcttcctgg acgattaatc ggtaggccgg atcaggcgtt acgccgcacc
    21961 cggcactagt ccctttgcac aaacgccacc ttttcggtgg cgttttttat cgcccacgca
    22021 ctaccagcgc ctggtccagc tcgcgatatg cttcaaccag tttctctagt gaaacgcgac
    22081 ttaaaccgct gggatttggc agcacccaaa tctgcgtcga accaatggtg agcgtttgtt
    22141 tcccccactg tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg
    22201 ccaacgcctg cggctgataa tcttcaattt tttcaatcag cttacgccca cctgcgtgca
    22261 gctcctgctt tgaaacttca ttggcttgca ccgttggacg gtctaccagt ttggtgacgc
    22321 cacaacgata atccagcaga tgctgtgcct cctgcggctt caactgacgg tcggtaaacc
    22381 cggcctgata tatcaccttc cagaagcgat ttgctggatg agcaaaggga aaaccagtcc
    22441 cggcggatga aagcccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa
    22501 tatcctcaac catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa
    22561 tacattgttt ataaaaacag caggcgcacg gtaatggctg gattgcgaca cggagttact
    22621 ttataatccg ctaccatggc cccttagctc agtggttaga gcaggcgact cataatcgct
    22681 tggtcgctgg ttcaagtcca gcaggggcca ccagatatag caaaggctga cgagaaatcg
    22741 tcagcctttt tctttttata tatcagttac tttgcgtgcc agtaagccgc tgcacgtacc
    22801 cgctgtgggt catactgttc cgcttcaaag cggcggctta aattcttaac gactttacct
    22861 tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca
    22921 tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga
    22981 taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca
    23041 agtttgctca acgtttccag gcggcggcgc agtgcgggca ttccggattc atcgcagaca
    23101 tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc tgccaccgta
    23161 agtttatcgc ccggttgcgc ctgcatcgcc cagccgctgg cgaccccacc gtcgtgaata
    23221 aagaaatcga tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac
    23281 agtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag
    23341 tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgatgtaaaa
    23401 ccgtccagcg cctcgccgcc gaggacaatg cgctgaaaac cggcgctgat gcgctcaacg
    23461 cgtaacacag tcagttcacg gaagcgcaga tcattgcgaa cgcgctgcgg gtagcggggg
    23521 gagttattca ttgttatcgc cttcgtgata gtaatcagat atatctaaat gattttcgta
    23581 aatgataatg attgttaatc atgataaatg caagcgattt gtagaactga tatatctgaa
    23641 gtatgtgaag gcattcagtg ccatagcaat gctgccggat gcggcgtgaa cgtcttatcc
    23701 ggcctggatg tgaggtgctt ccacgtttaa aaatcatttt tgtacttgca gtaatgctca
    23761 catcagatat aaattagata tatctaatca agcaaaagga ggctgatatg agccatcatc
    23821 acgaagggtg ttgtaaacat gaaggccagc cacgccatga gggctgctgc aaaggtgaga
    23881 agtcagaaca cgagcactgc ggacacggtc accagcatga acacggtcaa tgctgcggtg
    23941 gtcgccacgg tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc
    24001 tggtgattct ggatattctc tcgcgcgatg acagccacgg ttacgaattg attaaagcga
    24061 ttgagaacct aacccagggg aattacaccc caagcccggg cgtcatctac ccaacgctgg
    24121 attttctgca ggagcagtcg ctgattacca tccgcgaaga ggaaggtggt aagaagcaga
    24181 ttgcgctgac cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga
    24241 ttgaagaacg catcaaagcg cgttgcgttg gcgcggcgct gcgccagaac ccgcaaatga
    24301 agcgggcgct ggataatttt aaagcggtgc tggatttaca cgtcaaccag agcgatatca
    24361 gtgatgcaca aataaaaaag atcattgcgg tgatcgaccg cgccgctttt gatattacgc
    24421 aactggatta atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg cgacgcttga
    24481 cgcgccttat ccggcctaca cccgctacac accccgcagg cctgataaga tgcgccagca
    24541 tcgcatcagg cattgtgctc caaccgccgg atccggcata ccgattaatg cagcaccgtc
    24601 accgcgtctt ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct
    24661 tccaccagct cggcattttt ctgggtgatc aggttaagct catccactgc acgggtcagg
    24721 ctggaaagcc catcggcctg ttccagcgtt gaatggctaa tctgggcgat caactgggtg
    24781 acgtttttca cctgtgccac aatatcttcc atcgtccgtc cggcggcgtg tacctgctgc
    24841 gaaccggatt gcaccttatc agcactggca tcaatcagct tgcgaatatc gttggcagca
    24901 ttagcgctgc ggctggctaa atgacgcact tcccctgcca ccactgcaaa acctttgccc
    24961 tgttcgccgg cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac
    25021 gcaatatcgt taatcagcga agtaatggtg ccaatgcgct gggtactgtc ggcgatatcg
    25081 tccattgtct tgatcaccgt ggtcatcgcc tccccaccct gcactgccgc attactggcg
    25141 gtgatagaaa gtttatcggc agccgacgcc gtggcagagt tctgtttcac cgacgccgcc
    25201 atttggttca tggtcgccac cgtttgctga acgttatcaa ctgtctgctg ggtatgttcg
    25261 ttcagttcat cggtgccttt cgccagcgtc tcactgccat ttctgacact ggacacctgg
    25321 cttgagacat cgttaattag ccaacggcac atcaggccaa gctgccctac cgcgcgtaat
    25381 gtcagcccca gctcatcgct gcgattcagg tgctcaacac tattacgctc tccggtcgcc
    25441 accttcagcg cctgacgggc gacattttct atcgggcgca caatctgcca ttcaaaacaa
    25501 gcgcttgcca acaataccac taacacacag aggaaatacg tcaccaccgg ggcagcaaca
    25561 aaccaaagca tggccgccag caagataaac atcagggtca tcactccacg cgcccgccag
    25621 cgaagcggta atgaaggcag tttacccagc cagcctttac gcaccaccag gcctttatga
    25681 atacgcttac tggtacgtcc ggcgttcagc gctttgtaca gcggctccac cgccgcaatc
    25741 tcttcatccg tcgcccgggt acgaatcgac atatagccac tgatttttcc ctcgcgcacc
    25801 atcggtaccg cattggcccg cacccaataa tggtcgccat ttttgcggcg atttttcacg
    25861 atgccgctcc agggctcccc ttttttcagg gtgaaccaca tatccgcaaa cgccgctttt
    25921 ggcatatccg ggtgacgcac catgttgtgc ggctgccctt gtaactcttg caaggtaaag
    25981 ccgctcacct gcacaaaagt gtcattagca tgagtgatat agctttgcag atcggtagtg
    26041 gacatcagag tggtatcgtc cgccagcggg gtattttgct gggtgacata cggatgagaa
    26101 gacatgttcg cgtcctgtgc aggttatatg gttgttaact tcttgtcaga gtttatgtcg
    26161 gccccgctgc ggttatcttt aaccgattaa tttgatttag atcgcaattt gcgattaaag
    26221 cacaaatcta attctttgat ttaaaatact ttcactctgt tactatacga aaacgttaat
    26281 tatcttgccc aaaaatcagg caattattgc cctgaaaacg tgcatttgcg cagcaatcat
    26341 caaatccata cccgacaaaa accgtgcaaa ataacaacaa atgttaacag atagcattaa
    26401 atattgcaca aatgataacc gaatttgtgt ttatcccgat tttcgcgatc gcagccggag
    26461 tggcgcaatc cctgcaatac ttaaatcggt atcatgtgat acgcgagcct ccggagcata
    26521 ttttgaacag gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc
    26581 tcattgagaa gcgaacgctg gatcatgagg agatgaaagc acttaaccga gaggtgattg
    26641 aatacttcaa agagcatgtc aatccggggt ttttagagta tcgcaaatct gttaccgccg
    26701 gcggggatta cggagccgta gagtggcaag cgggaggttt aaatacgctt gtcgacaccc
    26761 agggacagga gtttatcgac tgcctgggag gttttggaat tttcaacgtg gggcaccgta
    26821 atccagttgt ggtttccgcc gtacagaatc aacttgcgaa acaaccgctg cacagccagg
    26881 agctgctcga tccgttacgg gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta
    26941 aactgaaata cagcttcttc tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc
    27001 tggcgaaagc ttaccagtca ccgcgcggca agtttacttt tattgccacc agcggcgcgt
    27061 tccacggtaa atcacttggc gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt
    27121 ttatgccact gctgccgggc ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca
    27181 cggctcttaa cgagtgcaaa aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga
    27241 ttcagggtga aggtggcgta attctgccgc cgccgggcta tctcaccgcc gtacgtaagc
    27301 tatgcgatga gttcggcgca ctgatgatcc tcgatgaagt acaaacgggc atggggcgca
    27361 cgggcaagat gttcgcctgc gagcatgaga acgtacagcc ggatatcctc tgccttgcca
    27421 aagcgctcgg cggcggcgtg atgccgattg gcgcgaccat cgccactgaa gaggtgtttt
    27481 cagttctgtt cgacaaccca ttcctgcata ccaccacctt tggcggcaac ccgctggcct
    27541 gtgcggcggc gctggcgacc atcaatgtgt tgctggagca gaacttaccg gctcaggctg
    27601 agcaaaaagg cgatatgttg ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc
    27661 tggtacagga agcgcgtggt aaagggatgt tgatggcaat tgagtttgtt gataacgaaa
    27721 tcggctataa ctttgccagc gagatgttcc gccagcgcgt actggtggcc ggaacgctca
    27781 ataacgccaa aacgatccgc attgaaccgc cactgacact gaccattgaa cagtgtgaac
    27841 tggtgatcaa agcggcgcgt aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt
    27901 aatatcaaat cggatggcga tacgacgtcg ccatccgatc tttttatacg acgcgtacgc
    27961 ccgcaggcat catccgctcc ggcgttaaca acacgctttc actgccgtca tcggtttccg
    28021 cacacaacag catgcattcc gacgtttcac cacgcatttt cgctttttgc agattgcata
    28081 agaccaccac cgttttcccc atcagctctt cttcgctgta gtacggcacc aggctggtca
    28141 cggtttgcag tgttttttcc cccacatcaa cctgtacgat gtacagcttg tcggcgtttt
    28201 catggcgttt cacttccaca atctttccga cgcgcatttc cagacgtgca aaatcagcgt
    28261 aagccacggt ttccattgtt tcctcccttc gggtaaaatt ttactaaact atagcaaaag
    28321 tttttctcaa tcctgtaggc taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg
    28381 ctgtgttacc gttacagcgt caaagaaacg cgctttattt actgaaaaca ggtgacccga
    28441 taagcacttc ctctacaatg ggggcgcaca tcagggaaag taaaaaaggt aaacatggca
    28501 acactaaaag acatcgcaat cgaagctggc gtatccctgg cgacagtatc cagggtctta
    28561 aatgacgatc cgacattgaa tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc
    28621 gaaaagctgg agtacaagac cagtagtgcc cgtaaactcc agacaggtgc agtcaaccaa
    28681 caccatattc tggctatcta cagctaccag caggagctgg agatcaacga tccttactat
    28741 ctggcgatcc gccacggcat tgaaacccag tgcgaaaagc tgggcatcga gctcaccaac
    28801 tgttatgaac acagcggctt accagacatt aaaaacgtca ccggtatttt aattgtcggc
    28861 aaacccacgc ccgccctgcg cgccgctgcc agcgcgttga ccgacaatat ctgttttatc
    28921 gactttcacg aacccggcag cggttacgat gcggtggata tcgatctggc acgcatcagt
    28981 aaagaaatca tcgacttcta tatcaaccag ggcgttaatc gtattggttt tattggcggt
    29041 gaagatgagc ctggcaaggc ggatattcgt gaggtcgcct ttgcggaata tggccgactg
    29101 aaacaagtgg tacgcgaaga ggatatctgg cgcggcggtt tttccagttc gtcgggttat
    29161 gaactggcaa aacaaatgct ggcgcgggaa gactatccga aggcactgtt tgttgcttcc
    29221 gattccattg ctatcggcgt actgcgggca attcatgaac gaggcctgaa catcccacag
    29281 gatatttcgc ttatcagcgt taacgatatc cccaccgcgc gatttacctt tccgccgctc
    29341 tccaccgtgc gcatccattc cgagatgatg ggaagtcagg gcgttaacct ggtgtatgaa
    29401 aaagcccgcg atggtcgcgc gctgccgctg ttagtcttcg ttcccagcaa attaaaactg
    29461 cgcggcacga cccgttaaat ccccttacac actgtccggc aatcgttttt gccggacagt
    29521 gctgccgttt attttcgtga tccagttaaa gtaaatgcat ttacctgcta ctttttagta
    29581 aaaattttac taaactcccc agcaattaca caaactacca tcaccatgaa tggttccgat
    29641 ttctctctac cgggaggccc tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac
    29701 cgacttgcgc cgcgtgcgta ctttttttca tatgattctg ttgcgcaagc gcgtaccttt
    29761 gcccgcgaaa ccagcagcct gtttctgccc ttaagcggtc agtggaattt ccactttttt
    29821 gaccatccgc tgcaagtacc agaagccttc acctctgagt taatggctga ctgggggcat
    29881 attaccgtcc ccgccatgtg gcaaatggaa ggtcacggca aactgcaata taccgacgaa
    29941 ggttttccgt tccccatcga tgtgccgttt gtccccagcg ataacccaac cggtgcctat
    30001 caacgtattt tcaccctcag cgacggctgg cagggtaaac agacgctgat taaatttgac
    30061 ggcgtcgaaa cctattttga agtctatgtt aacggtcagt atgtgggttt cagcaagggc
    30121 agtcgcctga ccgcagagtt tgacatcagc gcgatggtta aaaccggcga caacctgttg
    30181 tgtgtgcgcg tgatgcagtg ggcggactct acctacgtgg aagaccagga tatgtggtgg
    30241 tcagcgggga tcttccgcga tgtttatctg gtcggaaaac acctaacgca tattaacgat
    30301 ttcactgtgc gtaccgactt tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg
    30361 gtgctggaaa atctcgccgc ctcccctgtc gtcacgacgc tggaatatac cttgtttgat
    30421 ggcgaacgcg tggtgcacag cagcgccatt gatcatttgg caattgaaaa actgaccagc
    30481 gccagctttg cttttactgt cgaacagccg cagcaatggt cagcagaatc cccttatctt
    30541 taccatctgg tcatgacgct gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa
    30601 cgcgttggct tccgtgatat caaagtgcgc gacggtctgt tctggatcaa taaccgttat
    30661 gtgatgctgc acggcgtcaa ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga
    30721 atggatcgcg tcgagaaaga tctccagttg atgaagcagc acaatatcaa ctccgtgcgt
    30781 accgctcact acccgaacga tccgcgtttt tacgaactgt gtgatatcta cggcttgttt
    30841 gtgatggcgg aaaccgacgt cgaatcgcac ggctttgcta atgtcggcga tatcagccgt
    30901 attaccgacg atccgcagtg ggaaaaagtc tacgtcgagc gcattgttcg ccatattcac
    30961 gcgcagaaaa accatccgtc gatcatcatc tggtcgctgg gcaatgaatc cggctatggc
    31021 tgtaacatcc gcgcgatgta ccacgcagcg aaggcgctgg atgacacgcg actggtgcat
    31081 tacgaagaag atcgcgatgc tgaagtggtc gatattattt ccaccatgta cacccgcgtg
    31141 ccgctgatga atgagtttgg tgaatacccg catccgaagc cgcgcatcat ctgtgaatat
    31201 gctcatgcga tggggaacgg accaggcggg ctgacggagt accagaacgt cttctataag
    31261 cacgattgta ttcagggaca ttatgtttgg gagtggtgcg accacgggat ccaggcgcag
    31321 gatgacaacg gcaacgtctg gtataaattc ggcggcgact acggcgacta tcccaacaac
    31381 tataatttct gtcttgatgg tttgatctat tccgatcaga cgccgggacc gggcctgaaa
    31441 gagtacaaac aggttatcgc gccggtaaaa atccacgcgc tggatctgac tcgcggcgag
    31501 ctgaaagtcg aaaataaact gtggtttacc acgcttgatg actacaccct gcacgcagag
    31561 gtgcgcgtcg aaggtgaaac gctcgcgacg cagcagatta aactgcgcga cgttgcgccg
    31621 aacagcgaag cccccttgca gatcacgctg ccgcagctgg acgcccgcga agcgttcctc
    31681 aacattacgg tgaccaaaga ttcccgcacc cgctacagcg aagccgggca ttctatcgcc
    31741 acttatcagt tcccgctgaa ggaaaacacc gcgcaaccag tgcctttcgc accaaataat
    31801 gcgcgtccgc tgacgctgga agacgatcgt ttgagctgca ccgttcgcgg ctacaacttc
    31861 gcgatcacct tctcaaaaat gagtggcaaa ccgacatcct ggcaggtgaa tggcgagtcg
    31921 ctgctgaccc gcgagccaaa gatcaacttc ttcaagccaa tgatcgacaa ccacaagcag
    31981 gagaacgaag ggctgtggca accgaatcat ttgcagatca tgcaggaaca tctgcgcgac
    32041 tttgccgtag aacagagcga tggtgaagtg ttgatcatca gccgcacagt tattgccccg
    32101 ccggtgtttg acttcgggat gcgctgcacc tacatctggc gcatcgctgc cgatggccag
    32161 gttaacgtgg cgctttccgg cgagcgttac ggcgactatc cgcacatcat tccgtgcatc
    32221 ggtttcacca tggggattaa cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg
    32281 ggcgaaaact acgccgacag ccagcaggct aacatcatcg atatctggcg cagcaccgtc
    32341 gatgccatgt tcgagaacta tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc
    32401 tggacggcac tgactaaccg ccacggcaac ggtctgctgg tggttccgca gcgcccaatt
    32461 aacttcagcg cctggcacta tacccaggaa aacatccacg ctgcccagca ctgtaacgag
    32521 ctgcagcgca gtgatgacat caccctgaac ctcgaccacc agctacttgg cctcggctcc
    32581 aactcctggg gcagcgaggt gctggactcc tggcgcgtct ggttccgtga cttcagctac
    32641 ggctttacgt tgctgccggt ttctggcgga gaagctaccg cgcaaagcct ggcgtcgtat
    32701 gagttcggcg cagggttctt ttccacgaat ttgcacagcg agaataagca atgaggatca
    32761 tcgataactt agaacagttc cgccagattt acgcctctgg caagaagtgg caacgctgcg
    32821 ttgaagcgat tgaaaatatc gacaacattc agcctggcgt cgcccactcc atcggtgact
    32881 cattgactta ccgcgtggag acagactccg cgaccgatgc gctatttacc gggcatcgac
    32941 gctattttga agtgcattac tacctgcaag ggcagcaaaa aattgaatat gcaccgaaag
    33001 agacattaca ggtagtggaa tattatcgtg atgaaactga ccgtgaatat ttaaaaggct
    33061 gcggagaaac cgttgaggtc cacgaagggc aaatcgttat ttgcgatatc catgaagcgt
    33121 atcggtttat ctgcaataac gcggtcaaaa aagtggttct caaagtcacc atcgaagatg
    33181 gttatttcca taacaaataa caactacggc ggcaaaagga gtttgccgcc accgctaccc
    33241 tacacatttt cggagatgtg ttatgtctga taccaaacgt aatacaatcg gcaaattcgg
    33301 cttgctctcg ctgacttttg ccgccgttta cagctttaac aacgttatca ataataatat
    33361 tgagcttgga ctggcctcgg caccgatgtt tttcctcgcg acgatttttt attttattcc
    33421 cttctgtctg atcatcgcag aatttgtttc gttaaataaa aactcagaag ccggtgtcta
    33481 cgcgtgggta aaaagttcgc tgggcggacg ttgggcattt attactgcct atacctactg
    33541 gttcgtaaac ctgttctttt tcacctcact gttgccgcgc gttattgctt atgcttcgta
    33601 tgccttcctc ggatacgaat atattatgac gccggttgcc accaccatta tcagtatggt
    33661 gctgttcgcc ttctccacct gggtttccac caacggggcg aaaatgttgg ggccaattac
    33721 ctccgtcact tcaacgctga tgctgctgtt aacgctctcc tacattttac tggcaggtac
    33781 ggcgctggtt ggcggcgtac agcctgctga ccccatcacc gttgacgcga tgatcccgaa
    33841 cttcaactgg gcgttcctcg gcgttaccac ctggatcttt atggccgcag gtggcgcgga
    33901 gtccgtcgct gtgtacgtta acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat
    33961 catcctcgcc gggattttta tcggcgtact gtattccgtc tcctcggtgc tgattaacgt
    34021 cttcgtcagc agcaaagagt tgaaatttac cgtcggatcg gtgcaggtat tccacggcat
    34081 ggcggcgtat tttggtctac cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt
    34141 taccgcgatg ttcggttccc tgctgatgtg gaccgcaacg ccggtgaaaa ttttcttctc
    34201 cgaaatcccg gaaggcatct ttggtaagaa aaccgtcgaa ctgaacgaaa acggcgttcc
    34261 ggcgcgcgca gcgtggatcc agttcctgat cgtcatcccg ctgatgatta tcccgatgct
    34321 cggttccaat accgtgcagg atctgatgaa tactattatt aatatgaccg ccgcagcgtc
    34381 catgcttccg ccgttattca tcatgctggc ttacctgaat ttacgcgcca aattagatca
    34441 cctgccacgc gatttccgta tgggctcccg ccgcaccggt attatcgttg tttcaatgct
    34501 gattgcgata tttgccgtag ggtttgtcgc ttcgacattc ccgactggcg cgaatattct
    34561 gaccatcatt ttttataacg tcggcggtat tgttatcttc cttggctttg cgtggtggaa
    34621 atacagtaaa tatataaagg gattaacggc tgaagagcgc catattgaag cgacgccagc
    34681 cagcaatgtt gattaagcag aataataaaa aatgagcagg cattttccct cttcctggat
    34741 gaggagaggg aaaatagttt ctgcctttat atttttataa ttacaacgat aaaaggctgt
    34801 attttttctt tagctcatgg attaacacaa tgaaattaat cactgcacca tgcagagcat
    34861 tacttgctct gccgttttgc tacgcctttt ctgcggcagg agaagaagca cgtccggcag
    34921 aacatgacga cacaaaaaca cctgcaatta cctcgacatc ttctccttca tttcgttttt
    34981 acggcgaatt aggggttggt ggatatatgg atttagaggg tgagaataaa cataaataca
    35041 gcgacggtac ctatattgaa ggtggcctgg agatgaagta cggctcctgg ttcggcctga
    35101 tttacggcga aggctggacc gtgcaggccg accacgacgg caatgcctgg gtgccagacc
    35161 atagctgggg tggtttcgag ggcggaatta accgtttcta tggcggttat cgtaccaatg
    35221 atggcaccga aatcatgctc agtctgcgtc aggattcctc gctggatgac ctgcaatggt
    35281 ggggcgactt cacccccgat ctgggctacg tcattcccaa tacccgcgac attatgaccg
    35341 cgctgaaggt gcagaactta agcggcaact ttcgttatag cgtcaccgcc acacctgccg
    35401 gacatcatga cgaaagcaaa gcctggctac attttggcaa gtacgatcgc tatgacgaca
    35461 aatacaccta tccggcaatg atgaacggtt acatccagta tgaccttgcc gaaggcatca
    35521 cctggatgaa cggtctggaa atcaccgacg gcacaggaca gctctatctc acgggcctgc
    35581 taactcctaa ctttgccgct cgcgcctggc accataccgg acgcgccgac gggctggacg
    35641 taccgggaag tgaaagtggg atgatggtga gcgccatgta tgaagcgtta aagggcgttt
    35701 atctctccac cgcttacacc tacgccaaac atcgccctga ccacgctgac gatgaaacca
    35761 cctctttcat gcagtttggt atctggtacg aatacggcgg cggacgtttc gccacggctt
    35821 ttgatagccg cttctacatg aaaaatgcct ctcacgatcc cagcgaccaa atcttcctga
    35881 tgcaatattt ctactggtaa taaggactgt aatttcatga aaataaaaac tattttaacg
    35941 ccagtaacct gcgctctgct gataagtttt tccgcccatg ccgctaacgc cgacaattat
    36001 aaaaacgtga ttaaccgtac tggcgcgccg cagtacatga aggattacga ttacgacgat
    36061 caccagcgtt ttaatccgtt tttcgatctc ggagcctggc atggtcatct gttgccagac
    36121 ggccctaaca ccatgggcgg ctttccgggc gttgcgctgc tgacggaaga gtacatcaac
    36181 tttatggcca gcaatttcga ccgcctgacc gtctggcagg acggcaagaa agtcgacttt
    36241 acgctggagg catacagtat tcccggtgcg ctggtgcaaa aactgacagc aaaagatgtg
    36301 caagtcgaaa tgactctgcg cttcgccacg ccgcgcacgt cactactgga aaccaaaatc
    36361 accagcaata aaccgctgga tctggtgtgg gacggcgaac tgctggaaaa actggaagcg
    36421 aaagaaggga aaccgctttc cgataaaacc attgctggcg aataccctga ctaccagcgc
    36481 aaaatcagcg ccacccgtga tggcctgaaa gtcacctttg gcaaagtgcg cgccacctgg
    36541 gatctgctga cctccggcga atcggaatat caggtgcata aatccctgcc ggtgcagact
    36601 gaaatcaacg gcaatcgctt taccagtaag gcacatatca acggttcgac gacgctctat
    36661 accacctatt cccatctgct gacctctcag gaagttagca aagagcaaat gcagatccgc
    36721 gatattctgg cgcgtccggc gttttatctc accgcctcgc agcaacgctg ggaagagtat
    36781 ctgaagaaag ggttaaccaa tccggatgcg acgccggaac agacgcgcgt cgcggtgaaa
    36841 gcgatcgaaa cgctcaacgg taactggcgc tcaccgggcg gtgcggtgaa atttaacacc
    36901 gttacaccgt cggtgaccgg gcgctggttc tccggcaatc agacctggcc gtgggatacc
    36961 tggaagcagg cgtttgcgat ggcgcatttc aatccggaca tcgccaaaga gaatatccgc
    37021 gcggtcttct cctggcagat ccagcctggc gatagcgtgc gtccgcagga tgtgggcttt
    37081 gtccccgacc tgatagcgtg gaaccttagc cccgagcgtg gtggcgatgg cggcaactgg
    37141 aacgaacgta ataccaaacc cagccttgcc gcctggtcgg tgatggaagt gtacaacgtc
    37201 acccaggata aagcctggtt ggcagagatg tacccgaaac tggtggccta tcacgactgg
    37261 tggttacgta accgcgatca caacggcaac ggcgtgccgt aatatggcgc gacccgcgac
    37321 aaagcccaca acactgagag cggcgagatg ctgtttacgg tgaagaaagg caacaaagaa
    37381 gagacgcagt ctgggctgaa caactacgcc cgcgtggtgg agaaaggcca gtacgacagt
    37441 ctggaaattc cggcacaggt cgctgcgtcg tgggaatcgg ggcgtgatga tgccgccgtc
    37501 tttggtttta tcgacaaaga acagttggat aaatatgtcg ctaatggcgg caaacgtagc
    37561 gactggacgg tgaaattcgc cgaaaaccgc agtcaggacg gaacgttgct gggctactcg
    37621 ctattgcagg agtcggtgga tcaggccagc tatatgtaca gcgataacca ttatctggcg
    37681 gagatggcaa ccatcctcgg taagccggaa gaggccaaac gctatcgcca gttggcacag
    37741 cagctcgcag actacataaa cacctgtatg ttcgacccgg ctacccagtt ctactatgac
    37801 gtgcgtattg aagataaacc gctggcgaac ggctgcgcgg gcaaaccgat tgtcgagcgc
    37861 ggtaaagggc cggaaggctg gtcgccgctg tttaacggtg cggcaacgca ggccaacgcc
    37921 gacgcggtgg tgaaggtgat gctcgatcct aaagagttca acacctttgt cccgctggga
    37981 acggcggcgt taaccaatcc ggcttttggc gctgatatct actggcgcgg gcgcgtatgg
    38041 gtggatcagt tctggtttgg tctgaaaggg atggagcgtt acggttatcg tgatgatgcc
    38101 ctgaaactgg cggatacgtt cttccgtcac gccaaaggat taaccgccga tggcccaatt
    38161 caggagaatt acaacccgct gactggcgca cagcaaggcg caccaaattt ctcctggagt
    38221 gccgcgcatt tgtatatgtt gtataacgat tttttccgta agcaataatt tgcaactgct
    38281 ggcggatgcg gcgtaaacgc cttatccgcc ctacatgtgc aatcctcctg gccggataaa
    38341 acgcgacaag cgtcgcatcc ggcatctgca ccgtgccact ggcggatgcg gcgtgaacgc
    38401 cttatccgcc ctacatgtgc aatcccccag gccggataaa acgcggcaag cgtcgcatcc
    38461 ggcgttacca ccgggcgtat tctttttgaa tcccatcaca aaccccgcac tccccttttc
    38521 ccttttctcc ggcgacggct aaattagaac tcatccgacc acataacaat tattttacat
    38581 actggacaat tttatgagct acccgttgct gttcgccccg ctggatttag gttttaccac
    38641 gttaaaaaac cgcgtgttga tgggctcaat gcacaccggg ctggaggaat acccggacgg
    38701 tgccgagcgg ctggcagcgt tttatgccga acgcgcccgt cacggcgtgg cgctgattgt
    38761 cagcggcggc attgcaccag atttaacagg cgttggcatg gaaggtggcg caatgctcaa
    38821 cgacgccagc cagatcccac accatcgcac cattaccgaa gcggtacatc aggaaggcgg
    38881 caaaatagtg taaccaggtg aatcatttga taccgacaag gcttttagtg aaaggaggtg
    38941 aaatcacctg taagatgctg aaataaaaca ggtgatgtat agttcggacg gaaaactctt
    39001 tttgataccg actgctcaac aaatagtcaa aagatcaaaa aacggcgaca catttgatac
    39061 tgctaattta cccttaaaat cttcttcaaa acatcttcaa actgccaaaa tgctcacata
    39121 atcgatctaa ctataatcag tatgataatc atcatgaatt actattcaat agcgtgatta
    39181 tcttgggatg agtcatatga tctctatatg tatttacagc accacctcat gatagcgatg
    39241 gcgcttaacc aacaacattt ttctttactg tcactgccct tgattcctgg gtggcgttat
    39301 tgctcgagtt gtacttctca ataacgcgca aaatctcttt gtcagtgtct tcgcttcccg
    39361 ccattcctac ttttattgca ttttttagca ctggacgtaa cggtaacgta tcaatgatgc
    39421 tttctacggc atcctggctg atcgtagagg cattcattgc caccaatctt ataagggtgt
    39481 ttacgccctc acgcgatatg aaatcaacca gtaatacaca ttcttctttt ttgagcttat
    39541 gaagtaagtg aatgagtaat tcatactcat tgtcataatt ttgtttagca tcatctcgaa
    39601 ctattgtttg gtttgcttgt tgttctccag tcgcgagcca agtcagagat accccgcaag
    39661 cgtcagcaac aatagccagg ctatcaagtg cagggtaaat ctttccctgt aggtatttcc
    39721 ttattgttgt ttcggacatg ccacttcgtc tcgctaattc ggcattactc atccctttca
    39781 tggccacttt gagcctttca ccaaagcggt ttattgtgct ccggctaata atccgatcta
    39841 acttttccct tttcacatcg gttttttctt cataactatt tgtcataaaa atggttttcc
    39901 tacaaaaacg cacaaaagaa aaaccaaaca ataaaccgaa caaaaagctt gatctcggat
    39961 tattgttcga gtatcgttta cctaaatcga taaccggttt tgattattgg tttaggtaaa
    40021 catgagagga tcacacaatg agggtatcca gacaagcatc gaataatttg tttgttcgag
    40081 aggggtgtga ctggtctgct aaagcggtat atgcagccct tgaaaacaaa ggcacagacc
    40141 tccctgaact ggaaagggag ttaggattaa aaagcggatc aatgcgcaat gtgttttacc
    40201 gccagtgccg taaatacgaa gccgccatcg ccgaaaaaat aggggttgaa cctgctgtaa
    40261 tctggcccag cagataccag gtggacgcca gagaagtggc ttgatggggg atgtatgtgg
    40321 gtaacagcaa aagaatgtgt tggtcttcct gatttaccaa tgatggaaca taacattcgc
    40381 catcgactca caaaagcagc tgcgggaaat gcagccatgt tacgcaaacg taaggggaca
    40441 aaagcaatcg aatttaatat cgattgtttg ccttctggcg cgcaacaagt attgcgtgaa
    40501 cgttatgccc ttcaactgat gacgcaaaaa gctgatgaat caccggctcc ggtggtgaca
    40561 aaggccagac gctcgcctgc cgtggttgat gcggtggagg catatcgcgg atcaccacaa
    40621 ctgatggttg aacgccttaa tgccctgact gaaaaccagc gtcaggtggc tgatgcacga
    40681 atcgcgatcg tcagcgaagt gatgaaagtc gcgcaacacc ccggtttcag ctgtgcgaaa
    40741 gctatccggt ttatcgttga caacctggca cgttcgcaac tggacgagcg cattgtggca
    40801 atggttgaga cggcgaacgc caaaaaggga aacagccgtg cgttgagtga aataacgctg
    40861 aaacgctgga ttgcggcctt taacaaggcg cagaacgccg ctgaacgcct gcttttactg
    40921 gcaccgggta agcgccagga aataaaagcc gaagatatta actggctgcc cgaatttctg
    40981 gcgcagtatc gccagtcaaa cggccgacca atgaccgagg cttacgagga ttttgtcgct
    41041 gaatggcagc accggcacgc tgatgagcct tatatgctcg atatcatgcc gtcttatgac
    41101 accattcgcc gcgcaatgaa gaaactgccg gaagtggtga aacaaaaagg ccgggtgacc
    41161 ggcagtgaat accgccagct tgagggattc acgcgccgcg actggtccag aatgccggtg
    41221 aattatgtct ggattggtga cggtcacggc atgaagctga aatgcgcaca cccggttcac
    41281 ggtcggccat ttgcaccgga agtgaccttt gttatcgacg gtggcacgcg ctttgtggtg
    41341 ggctggagcc ttgacctggc tgaaaatgtt ttcgccgtag ccggtgccat acagcacggc
    41401 attcgccatc acggcaaacc gtttctgtat tactcggata acggctccgg ggaaaccgcc
    41461 gacgtcctgg ataaggaggt tgtggggata ctgccgcgac tggggattaa tcacccgacc
    41521 gggattgccg gtaatccgca ggggcgaggc attatcgaac ggcttaaccg cacattaccg
    41581 atgcgcatag cccgtaaata ccgcacctat ttcgggaaag gtgcagatcg cgagacgtta
    41641 cgcaaaacca accgcgattt acgctcggca tttaccgccc tgcaacaggg caaacggctg
    41701 aacgcccggc aacagtcagc gatgcgtgat ttaccgtcct gggctgaact gattgatgcc
    41761 attcgtgacg gtgttgagtg gtacaacaac cggccgcacg atgaattacc gatgaagccg
    41821 aacggtaaac attacagccc ggcggagttc agaaaaaaac gcctggcgga agaggacacg
    41881 gaaattgaat ggctgtccga tgttgagctg cgggatatgt tccggccgat ggtggaacgc
    41941 cctgtaagac gctgtgaaat acgctggctg aataatattt actacgcgcc cgagctgcgt
    42001 gacgagcatg gccgtaaggt gcttatcagc tatgacattc atgatgccga acgaattacc
    42061 gtacgtcgcc tggatggcag cgtgatttgc gaggcggtat gggacggcaa taaacgcgaa
    42121 gccttcccgg tcagcgcgga atactacaaa cagcagcaac gccttaaagg catgcgtaaa
    42181 cgcgcagagg aaaaaatccg tgatgccgag gatgaggttg tcaacgtgct ggagcacaag
    42241 ccgcaggagc cctggctgga aaacatatac cgccctgtgg gtaatacggt gaccgttcag
    42301 caaccggtcg ctgacgatga acctgatgaa gaatacgagc gtaatttcca gcggggattg
    42361 cagttgctgg aagcgaaatt aaaagaaaat gacccgctgg cctgaaataa aaaaataacc
    42421 cgagcggcga ctcaggttat ttgattaaac aaggtatcaa atgagaggtt aataatatga
    42481 ctgatattaa cgatgtaatc aagaccattg atgaacttat tgatggcggc gtactgacgc
    42541 agtatgccat cgccagagag gcgggaattt ccgacggcac attatcggct ttccgcaagg
    42601 ggaaatataa aggcgataac gccgctgtgg ctgcttccct gcgttcctgg tatgaaaact
    42661 ggaataaaca aagcgcactg ccggaaccgc cgcagtttgt ggaaacgcag acagtccagg
    42721 agctgcgcgc actgtttcag gcggttcgtc tgatgggctg tattaacgtt attgtgggcg
    42781 taccgggtgt gggtaaaacg gccactgccc gtaattactg ccaggagcaa ccaaacacct
    42841 ggatgatcac cctgtcaccc gcgcactcca gcgtcacgga gtgtctgctg gagctggccg
    42901 atgcgctggg gattgattac acccgcgcga acaaaggggc attatcccgc gccatccgcc
    42961 gtcgcctgat gggaacgcgt ggactggtga ttgtggatga ggcggatcat ctgggtattg
    43021 acggtctgga gcaactccgg gcaattcagg acgccacggg gatcgggatg gtgcttattg
    43081 gtaacccgcg cggattgttt aaaggtggac gccgcgcctt tgatgattta tcgcgcctgt
    43141 tcagccgtct tgcccgtaca aaacaacttc gcaaggccaa aaaggcggat gtgctggcca
    43201 ttgccagggc gtgggggatc agtggtgagg ccgagctggc cgtcatgcag gctatcgctg
    43261 aaaagccggg agcgttacgc gttctgacac atacgcttaa ccaggcgtgg ctcaccgcca
    43321 gcggtgaagg cgcggcgctg acagaaaaac atattaatgc ggcctttaaa gaggtttata
    43381 ccaaccctga attactctca caggtgtgat tatggcggta tttaatattc ctgatattta
    43441 cggacgcttt tacctggtta atttcgataa cgtgaaggtg atttcactgg ccgaaaataa
    43501 agaatgtggc gatttacttt ttgaatttaa tgaccgcaca cgaatggtga tatctgccgg
    43561 acttgatcgc gaaggtgcga cagacgttta cagcggaata tgccgttctg ttggtgcgaa
    43621 acaagtcagc taaatgaggt gttatatgaa tatgcaatcc tgcggtaaca aaatgaattt
    43681 attcgactcc ctgaacagcg cgcgccgtct gaccgaactt gccggtgcgg tactggaacg
    43741 cagtaagcgc tatccgcaac gttttgcact gaaaaccacg ccgccggtag gcaacgtgca
    43801 gggaaccggt gaaattgaaa tcaccataca gaccaacggc ctgcgccgcc gtgtgaaggc
    43861 cacccgcatc agcggctgca cggtttactg ggaggtgtga ggtgaaaaaa aatctcattg
    43921 catgggcgtg gtcgagtggt cttattgagt ttggttacgt cctgccggaa ggtgcattgc
    43981 cgatagttgc cggaaagcct gccacggtac ggcatgtgat tgaggttatg gcgcgtcatg
    44041 gacgtgatga acaggagcag ttgctggttc cggggatacc ggaagcggtg acggaggaag
    44101 aagcctttaa tgccatgatt cggttctgcc gtgaggtcag acgccgggtc agttatccaa
    44161 acagaacgag gaccagagga tgagtaaagt cgtacgcatt attttcgaat acaaggagca
    44221 cgttatccat aaaaacgctg atggaacagt gcgcatgggg gtaagtctgg acatacgttc
    44281 aaccgggata aagcagaaag gtgatggacc cgccatgatt tttggggtgg ttatgctcgc
    44341 ggaaagcaga gactttgctg aacttgtggc aatgaaagcc agtgcgctca tgaaagatat
    44401 gagcatgcgt tccggggtta ttaaaggtaa tgaatttaat cagcaggggt aattccatga
    44461 gcaaagtacg cgttattttt gaatttaagc atgtttcgca tgacgaaaaa ccggcaggca
    44521 atgactgtgt tgaagtgcat gaaaagattg gtgtggatgt aaaaacagaa cgtgatacga
    44581 ataacaggcc gacgtcactc tgtgacgttt atgcaagtat tctccagtat cacagtcctg
    44641 aaattattca gtttctctca gcggaatttc aggcatctgt acaggctttt ggggcggatg
    44701 ccatcattaa acgccaccgc gtgcataaag catcaggcac actgcaataa ggaaaaacaa
    44761 aatggcaaaa cgcgttacaa aattaaaggc cgcagcagag gcggcaccgc agacccgtga
    44821 agaggtcagc cgcgatatcc gcaccctggg cgatattcag cgagaggcgc tgcgcctgga
    44881 aacagcgatg aatgatgaag tggcagaaat caccgcccgt tatacgccgc agattgaaaa
    44941 ccttaaaaaa gaaatcaaag tgctttttaa ggggattcag gactggtgca aaaccaaccg
    45001 tgatgagctg acgaacggcg gcaaaaccaa aactgccaat ctgaccaccg gaacggtgtc
    45061 atggcgactg gggaatccat catgcagcgt cagtcgtgat gtggaaggtg tgattgaaat
    45121 gctgcgccgt atgggccttg agcgttttat ccgcacgaaa gaggaagtga acaaggaagc
    45181 ggtcctggcg gagccggatg cggtgaaagg gattgccggt attaaggtga ataaaggcgc
    45241 tgaaagtttt tatgtcgagc cttttgaaca ggacgccgga ctgaataaat aacaccgcat
    45301 taatcaatta aatatcactt cgttttaatt atggcgctcg cgtcagggga ctgcctgcgc
    45361 ctgtaaactg aaaataagga acaggatata tggcatattt ttatttcaaa ttagaccgtg
    45421 tgcagacaaa taaatatttc accaaatatc aacagactgt tttaccgcta cgcaacagta
    45481 ttcttcgggc attactgaaa aatacaggcg ctgccggatt gcgcttaaag ccgtttgcca
    45541 tggacgtaat cagtgagttt tatttttctg gtgctctgcc tgcgggctgg cgtaagcgcg
    45601 atgatgtggc ttttatcggg gacggaccgt gctttattgc cagacctgat gagtcatgcc
    45661 ctgaaggacc ggcgattgcc gcaatgattg aagccgctga acgcgagtta agaaagcgtc
    45721 ctgatttcct tgtctggctc tgtgaaaagc tgggggtaat gagaatcccc tccatgttta
    45781 acatggactc ctggtggacc ccgtcgctct cccgtgatgc cctgtgcgtg gtgtttaaag
    45841 taggcgctta tggcagggaa ataaaagggg gtattcctga agaatgccag gagattaaac
    45901 attctgaata tgtggcgctg acggaggaat aattcatgat tgatgcaaaa gtgcttgaag
    45961 gggttaaaaa ctggctgagt atttacgggc gtctgacctg cggcattctg gctgaaaaaa
    46021 tgaatatgcc gccatcctcg atggtttatt ttctgcgtga tgcggtcgat gccggggtgc
    46081 tgacggaatg taacggtttt tatgatattc cgcgtccccg cccggtgcag ccggttcgtc
    46141 gcaaatgcag ccaggaaggt gcggctgatg atgttcagtg gtgcagcttc agaaaatccc
    46201 tgccgtggat tgaggggcat gatattccgt caatggcgtg ggaatttgct cagggcgttc
    46261 tgacctgtga aaccgtttat gtggtggctg aagttgatga gcaggccatg aaagaaggcg
    46321 tgccccagtt tgtgatggcg tatatcgaca tacgcctggg tgtcattatc tgcggtttaa
    46381 gcggctggaa tatcacctcg cgcgttctgc gttacctgat tgttgaccgg acggccgcgc
    46441 ctgccgggat atctgcggag gtggcgtaat gttctttaaa acatcaaacc cttccgcgct
    46501 ggccgcgtgg caaaaatatc agcaggactg ccagaaagtt aaggatgagg caaaacgcct
    46561 tgaggccgtg ctgaatgttg cgtgccggtc ggtatttgta tccggtatca gtggcttttg
    46621 ttttaaaggg ctgcgcttta tggatgacaa atatcctttt catcgcgact tatggcgaaa
    46681 accgactgcg tcgaatggct ggagctgcac accgcgcaca tcacgtattc ccaaagccct
    46741 gcgcgttgcc tctgacgaac ttaacagtct gtggcgtgaa tattcgcccg tcacgtatgc
    46801 cagaaccgat gcactgttgt tctggctggg tattgatttc tcggcaatca tgtttggccc
    46861 tgtgaagtgg ttctgcgttg acgatgtgat ttaccttgag tgcggcgtaa aacccgcaaa
    46921 tcagaaaatg accgaaattc tgtctgatga gttttatgct gctgaaaagc gagtcagggg
    46981 gtgatgcatg atgatattac aacccatggg gagaaaaggc cgtgcacccg ctcatgttcg
    47041 cgcatggaca cctgaagaag atgcgctgct gattgcgctt tatccatcca ccccggttaa
    47101 ggatattgct gtcaggataa aaagaagttt ctggggtgta cataaccgga ttgttttatt
    47161 acgcggtact tacccggagt tgctcaaatg caaacgcctc agatttaaac ctgatgaaga
    47221 taaatttatc cgaaaaaatg ccagaacaat gaccggtaag caaataggag aatatctggg
    47281 gcgagaccgg gattctgtcc acaatcgggc gcgatatatt ggcgtaagca tgaaaaaata
    47341 cggagagttg ctgcccttca cccgcatacc tgacgatgat gttcgtctta ttcgtgaatt
    47401 acgggatgct gaatcaccac ggcgtcttac ctttagggaa atcggcgaga aatttgaatt
    47461 atccgagggc acggtgagtt ttatttatca ccgtcgtcgg actgccgaag atgttgtatt
    47521 acgggaatta atgccatgat aacgacatta tttgttgaat cagatgaacc ccttgtgtgt
    47581 gccgtcggaa tgccggtctg tggcggaacg ctgaccggtg tttatttcgg ggatttgcgc
    47641 ggttatccct ggcattcact gaatgatgct ttcccgcctg atatggaggc tgtcgtgctg
    47701 attgttcagt atggtgacca acaggaactg cgcatcggcc atatggggta tgaaggcttt
    47761 tttattgatg aagaaaccgg agcctgcctt gaagatgagg acggacaggt gacgcactgg
    47821 tgccatattt cagctttacc ggaatgacgg gaggtgaatg cgtgagaagt tatattgata
    47881 atgaaaaact gaagacaatc agcgactgtc ttagcttgct ggcaaaaata aaagaaacca
    47941 ttgaagagat taaatttcag cttgaatacg aaccctgtgg cgatgataca ttgcgtaact
    48001 ctgccagaaa agcactggct gtctttcaga aacaacgccg gactgttgaa taccgccttg
    48061 ctgtattgcg ccaggaggaa aaggaaagaa atataagacg tcatgaactc gttaatgatt
    48121 ttcttgttcg tgaattaaag gagcgtgtgc cggagtcggt attttttgag tgtgaggctg
    48181 ttgcccgtag caaagcactg gaaactgatt aaacaggcag gtgaatgatg agtattgaat
    48241 taagatcgtc ttatgaatat cgtaaaattc tcatcgccgg aggcatgaaa ccggaagatg
    48301 cagaaaaaat cgtttctttt atggataaag aatgtgacaa acgggatatg ccagaaatta
    48361 ttatggatga catgattctg cattcagccg tagcgttaag cccattatgg attgtgcatt
    48421 ctcttgctga aattgccaaa ggcaccgata aacaggcagc tgttgctgcc cttcagacgc
    48481 ttaatgaaat gcgtatatct ccacgcccga cattaataca tatgattctg tccagcatgg
    48541 aggataaagc aaatgaatag tctacccgct ggatgggcgc ggccactaat ggccaggaag
    48601 catcattttt ttaaaacagg cgaaaatatc agtatatgtg gacggtggtt atatctggct
    48661 cataatcgcg agccggatac atttgaaagc cctgatgact gtgccgaatg ccgcagaaga
    48721 ctgaataagg agaaagataa tggacagtag ttcgcagttg ttctggtatt tatatatcac
    48781 atcctttttc ggtgcattcg tcattacacg atggctgtgc cggaaagtta tcagtatttt
    48841 tcataaaaaa tacctgattg ggcaggctgc gacttctctg attaatgcac tggccagata
    48901 tcataagggg gaatgctact caagaataaa aactgatgat ggttttaata tttttgtgat
    48961 tcctccggag tatcgggttc agctcgatcg ggaaaataat acctatcata tccgcaggag
    49021 tgttgataca gcatgagagg gaaactgata tctgccattc atgtggcaaa acgcgagctt
    49081 gccctggatg atgaaaccta cacatccgta ctactggcgg tcaccggcaa aaccagctgc
    49141 cgggatatgt caccggatga gttatcccgc gtgctggatg ttttcaaaaa acgcggtttt
    49201 aaagtgcgtc agaacccggt taaccgggcc ttaaaaccgg gtacggtgac cgctaaaatt
    49261 cgcgccatct ggaaggtgat gcaccggcag ggctttatct ctgatggtgc ggaaaccgcc
    49321 cttaaccgct gggtgaaatc gcagacggcc gcgcaaaacg gcggcgaagg tgtggcaaac
    49381 tggcagtggc tggagcaaca ccccgccctg gcctcagatg tgctggagcg tctcaagcga
    49441 tggcaccgcc gcaaaatgct ggccgcgatg ggaatgcccg aacgcacgct gatggggtat
    49501 gacgccgttt gcaggcagta tgaaaaatca cttccccgtt aaccccaaat cccgccacaa
    49561 cgcgggattt ttattttaaa cttaccggga acgcgagaac cggaggctga tatggcagaa
    49621 actcagatga gcatgtttgg tggtgacagt gagcaactgc acgcccttat cgaccgcctt
    49681 gatgacatcc ctgatgatgt tctcaaaaag aactggccgc ggaccctgtc agaactggtt
    49741 gaagtcaccg gcgcagaact tcagcgtcag gggattgagc cggtactggc cggcaaactg
    49801 gcacgcaagg tggctgcggc tcaggcagcc tatatgggcg gacggggtta ttacctgccg
    49861 gtcggggaat ctctctttgc cgagctgcga aacaatgaga tattttcgcg ctgggaccgg
    49921 ggcgagaaaa ttgaatccct tcgccgccat taccggatgt cagaaaccca gatttatacc
    49981 gtcatacgtg aacagcgccg tctgcatctg gcaagaacac aaccgccact tttctgatat
    50041 tccgcaaagc ctgccgcctt tccttcaccg ttacgctgac tcagagaaca tcatgagaca
    50101 gcgtaacaat gccaaaactc cccgcaccac tgcgtaaaaa gctgattgcc cttgttctgg
    50161 ccggtgccgg gacgttcacg attgccacgc attacaccgg ttactgggaa gggaaagaaa
    50221 actccacgta tatcgatccc accggtacac ccaccatctg ttacggccat accggcccgg
    50281 atgtgaaacc gggtatgacc ctgacggatg aagaatgcct ggaactactg gaaaaggaca
    50341 tgaaatgggc ctttgcggcc attgatcggc gtgttcaggt gccgcttacc cgtggtcaga
    50401 cggtggcgct ggcttcgtgg atcttctggg ccggtgaaac gaactttcgc aactccacgc
    50461 ttctgcgcct gatcaatgcc gggcagatgc ccgcgtcctg taagcagtat atccgctgga
    50521 tttattcaaa gggggtgaag ctccccggcc ttgaggcccg ccgttcggcg gatgaatggt
    50581 tatgtcgcta cgatttgccg aaagtctgaa ccgcttctgg cgaccgatca tgatagcgct
    50641 gctgtgtgcg gtactgctgt tacggggtgt cctgtggctg cggtgaataa tgccttaccg
    50701 gccctgattg ccggggcgtg catggcggcg ctggggatta tcgctgtact ggttgccgcg
    50761 atatggggaa tgcaccagaa gacgcagcgc cttgaagaca ataatcaggt gcttgtgcgt
    50821 gagcgggatg aggcgcgtca ggtgctggca aatcagcagc acaccctgca actcatttca
    50881 caaatcagtg aggcggccac gaatgaaaaa caacagaaca ttcagcacag cgaggggcag
    50941 cagagcgttg tccgccggtc gctggcagca gtgcctgcgg cctctgcccc tgttcctgat
    51001 gatgtggctg atcgggtgcg ccgggccgtc tgtgaaatac gtgcctgtga agccggtgcc
    51061 gatccccgct gaatggctgg ctgactgcct agtccctcct gcgccggagc cgttcacgtt
    51121 tggggcatcg gtcacttaca acctgcaact gctggcggtg atcaagaact gcaacgtgga
    51181 caaggccagt attcgtcgtc tggagacgcg gcgacaacat gaatttactg atatggccgg
    51241 aacgcctgct gttccggcag gaaaaacgaa gtaaggaaaa ggtatggatg attcagatcg
    51301 tgctcaggct gtgatggagc ggggatcaga acgcgccctt tgtaaccggc tgacacgtaa
    51361 gcgccaggca gtggatacgc cgggcagacg tgtctgcgcg gactgtggtg gagagatccc
    51421 cgccgcacgt cttgttgctg tgcctgatgc catccgttgt gtgaactgcc agaacatcat
    51481 ggaggcccgt aatgtgggtc agcatcgtta aagattatgt tgtgccgatc ctttcggcga
    51541 cggcaaccgc tggcgggata ttcatggcgc tgatgcgcaa aacgtttgtc ccccgcgagg
    51601 cctttgaaaa actctccgac cgcgttgaga aggtggaaac ccgcctgtcg tcactgccga
    51661 cagaagccga ggtcaaccgt ctgaatgtgg aaatcgtgac cctgcggggt gaactgaaaa
    51721 ccacgaacgc cacgctccgc tctgtctcct atcagaacga actgctgctg gagcaggctg
    51781 taaggaaaaa aacgcaatga gtgatttcat tactgaagat cagcgtctgg tcattctgcg
    51841 gtcgctggca gattacaacg gtgaactggg tgaatccgtg ctccaggact gtctggatga
    51901 ttacggccac cgcgtgtccc gcgacaccgt tcacacccac attgcctggc ttgccgagca
    51961 gggactggtg cgcaaacgcg ttctgattaa cggttatttc atcgcggaac tgaccggtcg
    52021 tgggcaggat gtggcggaag gccgggcctg cgttccgggt gtgaagaaac cacgcgcaag
    52081 ggggtaacga tggacaagcc gacgcgtggt cgtgtacgca aggtggattt gctccccgac
    52141 agcatccgta agccgctgct ggaaatgctg cgtgaaaaac gtctgacgca ggtccagatc
    52201 cgcgaggaaa tcaaccgtct gatccgagag gcgggcctgc cggaagagca gcagctttca
    52261 ccggcggcca tcagccggga agcctcccgt aatgagctga ttgcccgtaa cctgcgtgat
    52321 ttacgcgagc agacaaaagc catgatggcc gaactggggg acaagccaac cggtgaaacc
    52381 acagcgctga ttctggaaat gtcccgcgcc ctgatgtacc gccgcctgcg tgccgcgacc
    52441 gaatctctga acagcgacag cgacgtggat atgcgcctca tcaaagacat cctcctgtca
    52501 gcacagcgtg cggaaagtgc cgccgagcgc agcattaagc gtgaaaaaga aatccgggcc
    52561 gcatttgctg aagagatggc aaacgcggtc accgacgagc tgcgcggcgt ggacgggatg
    52621 agtgaacagc ttgagtcccg tattaagggg attctgttgg gtaaagcctg atgaataacg
    52681 ccgaagaact ctcaccctta ctgacgaata ccgtctccac ccgcaaaatt gatctggccg
    52741 gtgaaaaagc cctgctgggc gtggatgtgc cggacagcct tgatttgccc ggtgatatgc
    52801 cggtgtttct ggattatcag gcccgctggt ttgaggatga aagcgaggtc tgcatcgcgg
    52861 aaaaatcccg ccgcaccggg cttacctggg cggaagccgg gcgtaacgtc attaccgctg
    52921 caaaaccgaa gcggcgtggt ggccgcaatg tgttttatgt ggggtcaaag caggagatgg
    52981 cgctggaata tatttctgcc tgcgcactgt tctcccgtgc ctttaaccag ctggcagatg
    53041 ctgatgtgta tgagcagacc ttctgggatc gggataaaaa agaagaaatt ctgacctaca
    53101 tgatccgctt tccgaacagt ggattcaaaa ttcaggcact gtcttcccgt ccgtcaaacc
    53161 tgcgcggcct tcagggggat gtggtgattg atgaagccgc gttccacgag tcactggatg
    53221 agcttctcaa ggcggcaatg gcgcttacca tgtggggcgc gcgcgtgcgt attatctcca
    53281 cgcataacgg cgtcgataat ctgtttaatc agtacattca ggaagcccgc gaggggcgca
    53341 aggattacag cgtccaccgc attaccctgg atgatgccat tgcggacggg ctgtaccgtc
    53401 gtatctgtta tgtcaccggc cgcgaatggt caccggaaag cgagcagaag tggcgtgatg
    53461 atctctacaa aaacgccccg acccgtgagg atgccgacga ggaatacggc tgtatcccga
    53521 aaaaatccgg cggtgcctat attcctcacg cgcttattga aatggcgatg atccgcgaca
    53581 tcccgattct gacgtttgaa gccccggacg acttcatcag ccgtgccgca tggctgcgtg
    53641 aatcggaggt tttaacctgg tgtgaagaac atttaaaacc gcttttagag gcgttaaatc
    53701 cccgttcccg cttcagcttc ggggaagact ttgcccgcac cggagacctg tcctgctttg
    53761 tgctgctgga aatcactgaa tccctggcaa aacgcgaggt gtttcgcgtg gagctgcgca
    53821 acctgccgta tgcccagcag gagcaggtga tgatgtacat cctgacccgc gttccggcgc
    53881 tggtcggtgc ggcgttcgac gccaccggta acggcggcta tctggccgaa gccgcgctgc
    53941 tggcctttgg cccggacatc atcgactgcg tgatgttatc gccgaagtgg tatggcgagt
    54001 ggatgccaaa actgaaggcc gagtttgagg atcagaatat cctcgtcgcc cgccatcaga
    54061 ccacgcttga tgatttgcgc catgtgaagg tggtgaacgg tatcccgcaa atcgacaagg
    54121 ggcgtacgaa ggaccagaac gcaacggctg tgaacgcccg ccgccatggt gattttgccg
    54181 tggcgctctg tatggcaaac cgggcgtcat acatggaggg cttcatcctg gatgaatcag
    54241 cctgtcaggc gctgccggag cggtcgcggg caatggaggg cggttatcgt gatgacgatg
    54301 aggcatatca tgaatttgat cgggggtgct ggtagtggga cgcataattg atcttgacgg
    54361 aaaacctttc tcctttgacc cggagatgca gagtgccgtg ctggatattc cgcagattgc
    54421 cagccgttat attgaacatc cggcctcggg tatcaccccg aaccgggcgg cacagtgcct
    54481 tcgcggagct gaacgtggcg atctgattgc ccagtccgat ctggccgctg acattgaaga
    54541 aaaggatacc cacctttttg cagagctggg caagcgacgc cttgccattc agggggtgtc
    54601 ctggagtatt gagccgcccc cgaacgccag cgcgaatgag aaaaaggacg cggaaatgct
    54661 cgacgaatat ctgcattccg ccgactggtt tgatgccatg ctgtttgatg ccaccgatgc
    54721 catcctgaag ggctattcct gtatggagat tgagcacggg atgctcggta aaatgcacat
    54781 catccgcgcc atccgctggc gtgacagcgg gcatttctgc cttaacccgg atgatttgag
    54841 cgaactgcgg ctgcgtgacg gcagccatgc cggggtggcg tttcagccct ttggctggat
    54901 agtgcatcag tcgcgttcac gcactggcta cggtggtgcg acggggcttg tcagaacgct
    54961 tatctggccg ttcattttca aaaactattc cgtgcgcgat ctggctgaat ttctggaggt
    55021 gtacggcctg ccgatgaagg tcggtaaata cccgtccggg gcaacaccgg agcagaaaag
    55081 cgccctgatg cgggcggtga tggatatcgg gcgacgtaca ggcgggatca tcccggccgg
    55141 gatgtcgctg gagtttcagg cggcagcgaa cggtcaggcc gatccgtttg aaaccatgat
    55201 ttcgtggggg gagcgttcca tctccaaagc tatcctcggc ggcacgctga ccacggaagc
    55261 cggagacaag ggcgcacgtt cgctgggtga agtgcataac gaggtgcgcc gggaaatcag
    55321 ggattctgat ttacgtcagc tggccgccac gctgaaccgc gatctggtgt atccgctgta
    55381 tgccctgaac accgcccacg ccattgatat ccgtcgcctg ccgcgtatct gtttccagac
    55441 aaaagagccg ggggatatca ccaaaatcac cagtgcggtg atgcagctca gtacggggat
    55501 ggatattcct gatccctggg tgcgggagca gaccggtatt ccacagcctg cccccggtga
    55561 agccatcttc cgtgtccgtc agagtggcaa tgaacctgct cagacagaca aagagatgcc
    55621 accggaaaaa caggagaaga cagagcagac agcgctggct gccagactgc cggaagcgaa
    55681 gagcagcccc cgagatgaac tggacgacat gggggatgcg gtgcctgccc gccggttaca
    55741 ggaggccatc gacccggtac tggagccggt cattgaagcc atcagaacgc gggggctggc
    55801 ggaggcactg gcagacctgc ctgcccttta ccgtgaaatg gatgattccc gcctgatgac
    55861 gctgctcagt gatgccatgt ttgctgcgga aatgaagggg atgctggatg gcacagggga
    55921 ttgatttagg ttatgccgcc acgcttccct caaaagaggc ggtggcatac ttccgcgcca
    55981 aaggggcgca tatcagctgg aactggttcg aaacagacgc ggatgttcat gcccgttcat
    56041 tcacggcggc aaaagtggca cgcctggacg tactgaccac actacaggcg gaagtgcagc
    56101 gtgctgttga tgaggggatt tcacagaaag catttatccg cacactgaca ccccgcctgc
    56161 aaaagctggg atggtgggga aagcagattg tggtggacag cgccggtaac gcagaagaag
    56221 tgcaactggg cagtccccgc cgtctggcgc tgatttacaa cgtgaacacc cgcgtggctt
    56281 acaatgccgg gcgttacacg cagatgatga acaacacgga cacgcatccg ttctggcagt
    56341 atgtggcggt gatggacagc cgcacccgcc cggcgcattc cgcccttaac gggcaggtat
    56401 tccgctatga cgatccgttc tggaatacac actacccacc caatggctgg aactgccgct
    56461 gccgtgtccg gccattgtct cagacccgtc tggatgcaat ggggttatcc gtttcatccg
    56521 gtcaggatca tctttccacc cgcaatgttg aggctggcgt ggataagcag accggagaag
    56581 tcagagaaat gccggtgacc acatattcag atggcaccag aaccatgaca ccggatgtgg
    56641 gctggtcata taaccccggc tcggcggcgt tcggcacaga ccaggcgttg atccgtaaac
    56701 tgatcgaggt gaaaagcccg gcgctgcggg aaatggtagt gcaggagatg aacaacagcc
    56761 cggagcggca gctggcgttc cgcatctggg caaaaaacat catgaaaacc cggcgaggcg
    56821 gtaacgatat ccgcacgctg ggctttatga ccgaaagcat tgcgcaggcg gtggaaagcc
    56881 ggacgggaac gccaccggcc cgcctgctgg cgatgagcgg taaaaatgtg ctccatgcgg
    56941 acagcatgaa acatcagaat gacggtatcg ccctgacgcc ggaggatttc ggtcgattac
    57001 cggcaatgct ggcaaagcca aaagcggtgc tgtgggacaa acgccacaac aatctgatgt
    57061 atatcgtgga aagtaaggat agcagtgtgc agattgccgt taatgcgccg tacagcctga
    57121 agcgtcagcc ggataaactg gatgtgattg tgaatgctta ccgggttatc aatatggata
    57181 agctgaaatc ggatatccgg ggtggaatgc ttgaggtact ggaaggagat attgattaac
    57241 ggtcagtggc gggggttgaa cccgcataca tgatccaccc ggaggcaggc accgacttta
    57301 ccagttaagc gtacactgac cgcctgttta ttttatattc atcctttatg cggaggcaat
    57361 atgtcatcca ttgatgccgc tgtcgttgtt gacgttacgc gtctccagcg ggtctttgcc
    57421 cggcttcagt ttgtgggtgg agggaaagac ctggcccgca gtgtggcggc gagtcttctg
    57481 tcatcgtcag aaatggcgtt tgaacaggaa aaagagccgg acggcgaacg ctggcatgac
    57541 tggtcagatc cttaccgcaa gtggcgtacc cgtaagggat acatgcccgg caaaatcctg
    57601 acgctgaacg gcgatctggc ccgacgcctg accacggatt atggcgatac ctgggcgctg
    57661 attggatcaa atgagcctta tgcggccatt catcagtggg gtggcctgcc tggtatgcca
    57721 ccgggaccgg cggccattgg cgcacgcccg tatatgggct ttgatcaggt ggcagagcag
    57781 gagatcatgg acgaaatcag aaaacgcttt aaaaaggcca cagaaacgcc ttaattgttt
    57841 aaggtatgca aatgcattac cttaccccct tcaggcgcgt gtcgtgattt tctaacctgt
    57901 atttaacggg ctttaaaatc tgcgtgaagc gcctttgtat ttttcccctg gaagcacttc
    57961 ctgaaatccc gcaaaacccg ccctgaaaat tcccggctta tgctgccgga atgaagacga
    58021 aaaacacgcc caaacttgct tatgccattc tgaatgccat cagcctgtcc gcagacgggg
    58081 acggtgactg gtgtcagatc atgccagccg gtcgggtaaa ggcccgtgac ggtcgcccgg
    58141 aaaaaccggc cgagggctgg ctgattaacc gtgcgaccgt tgagcgcatg gtatcccggg
    58201 ttgtggcgct caatcagccg gtgaaaattg actacaacca ccagaccctg attaaggggc
    58261 atcaggcacc ggctgccggt tttgtgatgg cctcaccgga gaattttcgc ttcagtgaag
    58321 agcgtggctt cgaggtgcgc ccgaagtgga accctccggc ccttgaacat ctgcgtaata
    58381 acgaattccc ctggttttca ccggtgattg gctatgacga gaacaccggc gaacctgtcg
    58441 aacttcggat gctggctatt accggtgacc ccggtctgac cggcatgaat cctgtcgccg
    58501 cgctgtcggc ggatgacctt tataacgcct taaaccctcc tttaaaggat acctccatga
    58561 atgagcaatt acgccagttg ctgacggcgc tcggtctgac cgtggctgac ggtgacgaat
    58621 ttacgccgga gctgggcacg gcggcgctgt ctgccctcac cgggatcaaa acccgtgcgg
    58681 atgcacacga caacctgaaa acacaggtcg ccagtctgtc ggcagagctg gaaaccgcaa
    58741 aaggcacacc ggctggcggc attattgatc tgacgaaata cgtgcccgtt gaaacgtata
    58801 acgccctgcg taccgaatac gttgcgctgt cggcgcagca cggcagcacc acgctggagc
    58861 aggtgctgga caaggctgaa tccgaagggc gcatcttcaa aagcgaacgc ggctatctgg
    58921 aacaactggg cgggcagatt ggcgttgcgg cactttctgc acagcttgac gcccgccagc
    58981 cggttgcggc cctgacctct cttcagaccg acaccgtgac cgtgccggat aaaaagactg
    59041 ccaccgctgc gctgtcggct gaagatatcg cggccgccaa actgctgggt aaaaccgaag
    59101 ccgagttcct gaaaatgaaa gaggaaatgc aataatgcct accccgatta caccggcgat
    59161 gatcacagcc ctgatgacgg gctaccgttc tgattttcag gccgggatgt ccatggcccc
    59221 gtcgcagtat aaaaaaattg cgatgaccgt gccctccaca tcgaaatcca acacctacgg
    59281 ctggctgggg cagttcccgc agttccgtga gtggatcggc tcccgcgtca tcgagaagat
    59341 gaaagcctat ggctatgcca tcgtgaacaa aacttttgaa ggcactgtcg ccattagccg
    59401 cgatgacttc gaagacgaca acctcggtat ttattccccg ttgttccagg agatggggcg
    59461 cgctgcggcg gtacaaccgg atgagctggt ctttgctgcc ctgcgtgacg ggatcagtgc
    59521 ggcctgctat gacggtcaga acttctttga taccgaacat ccggtttacc ccaaagtgga
    59581 tggctccggg gatgcgcaga tggtcagcaa tatgtttgtg gcaaaaaccg gctctgtcgg
    59641 cgcacaggct gattacagcg gtccggcctg gtatctgctt gactgctccc gcgcggtaaa
    59701 accgctgatt tatcaggatc gccgtaaggc tgaactggtt gcccagacca aagtcgatga
    59761 aggtcgcgca ttcaccgata acgaatttgt gtttggtgca tccgcccgtc gcaatgtggg
    59821 ctacggcttc tggcagatgg cctacatgat gcagtcaccg ctgacgctgg atgcactgtg
    59881 gcacggctgg tcagccatgc gcgaatttac cgctgacggt ggccgcaaac tcggcattaa
    59941 acccacccat attgttgtcc ccacctcgct ggaaaaacag gcggtgcagc tgctggagcg
    60001 tgaactgttc gcagacggaa acgcaaccgt ctctaacgag atgaagggta agctggagct
    60061 ggttgtcgcg gattatctgt aaacggtgag ccgggcttac ggcccggccc ctgcggaggg
    60121 caaatatgaa tgaacatcac actgtggcag caacagatga ttcaggtctt caggtcagtg
    60181 gtgacaattc tgtcacggtg ctggcagaag tgcgttgcag tcgttcggcg tttcgtcgtg
    60241 cggggttcct gttcacgcgt ggacgtcagc aggttgaggt caccccggag cagcttgccc
    60301 gactggagga ggagccatgc ctcactgtgc gaatactaca gacgtctgct gatgatgcgg
    60361 ggagcgtggc gggtgtggtt catgcagtgg ccggtacaga tttagccgaa gccgaagccg
    60421 aagccgaagc cgaagccgaa gccgaagccg aagccggaca ggatgctccc cggaaaaaga
    60481 ccggtaacaa agcggaacag gcccgtgcat gaattacgcc actgaaaccg atatgcgggc
    60541 gcgttaccgc gaggatttgc tcaggccgtt actggctgtg ccccgttcgg atgagccgga
    60601 cacgcgcaag ctgaaccggg cgctgacaga tgcgtcagtc cttatcgaca gctatctgtc
    60661 cgcccgttac acgttgccgc tggaggttat tccggccgtt cttgttcagc actgttgcgc
    60721 gattgccttt tattacctgt gcgatcagcg agcctccgat caggcgcgtg accgttaccg
    60781 tgaagcgctg gcctggctga aagatgtcat gaacggcaat gtgccggtcg gtgtggatac
    60841 gaacggtgcg gcccctgaat ccggggattt accacaggtt cagtctgatg cagcggtatt
    60901 cgggcgcaac cagaagggct tcatatgatt acggaaaccg aacaggcata catcgcccgt
    60961 atccgtgagt atttcgggaa tgaactggtg tctgttgaca cgcatcccgg cgactggagc
    61021 gacagcgtac tgcgcaccat gctgattaac gccccggcga tctacgttgc ctggctgggt
    61081 gccggtgaag gccgtacccg tggtcgcctg gtcagtcact gggtgttcta cgtcatcggc
    61141 gacatgctca acgggcgtga ggccagccgt cccggactgt atcagattgt ggcccggctg
    61201 attgccgtgc ttaacggctt cagaacagaa aaaacctcac cgctttactt tgaaaaggcg
    61261 gtcaacggtt acaccgaaac ccaggcggac agtggtgcgg tgatgtatgc gctttatttc
    61321 tcctgcgagg aaatgatcgc accgctgacc gataccagct cgctggacga cttcctgcgt
    61381 cattacgaaa cctttgtgga gccgcccggt acgccgccgt ttgaggcaca tattgatttg
    61441 ccggggaaca cgacaacggc ggtcgaaccg ccggaggaac tgtaaatgaa aaccatcttt
    61501 atcaaacccg cgccgggacg cctgattcgt gatcccgaca caatgcgccc gctggcacag
    61561 gagggggagg aaaagccctt tacgccgttc tggtgtcggc gtcttgatga cggcgatgtc
    61621 attcaggctg aaaaagccgc tgaagaggct ccggctgtat ctgcggatgc aacgactgcg
    61681 acagaaaaac cggtcgccca accggcatcc gataaggaga aaccgcaatg attaattttg
    61741 acgggatcgg taatgaccgc cgtataccgc tgattgaggt tgagtttaat aactccatgg
    61801 ctgtcaccgg cactccggca cagcgtcagt gtgtgctgct gtttggtcag gcgagaatga
    61861 aagagaatgc cgtggacggt gccggtgttc tggatgttcc ggtcaggatc acccgtgcgt
    61921 ctcaggcaac agaactctgg gggcgcggct ccatgattgc cctgatggtg gcggaattta
    61981 tcgccatcaa tcctgatgct gaactgtatg ccattgctca ggggaacggc accggacagg
    62041 caacggctgc ggccatgaat ctgaccggaa cggtcacccg tgacgggatt gtttatgcct
    62101 gcgtgggggg gcggcgttat acactcccgg caccaaaagg taagaaaggc aaagaactga
    62161 ccgacgagct ggccgcgctg atcaatgccg atcctgacgc gcccttcacg gcctcctctg
    62221 gtgcaggaag tggtgataat ggcgcaggtc tgaaagggtc tctggggatc accgcccgct
    62281 ttaccggtga atgctccgtt catgatgtgc gcctgaacta tcacgacggg gaagcaaccc
    62341 cggaaggaat acaggtcgct atagcctatc cgaaacaaaa agcggctaac ccggatatca
    62401 cccgaagcgt ggcgggtatg ggcgatcgcc agtacaacta tgtcgtcatg ccctataagg
    62461 atgatgcaaa cctgaaaatc atcagtgatg agcttctgaa acgctgggga ccggccaaaa
    62521 tgtctgacgg cgtgctgtgg ctggcacata ccggcacgtt tggtgaggtg caggcattcg
    62581 gggcaaaacg caatgatttt ctggcgacct gcacatccat tcccaaagcc cctgaaccgg
    62641 attacctctg ggcagcggcg gtctgtgcca cctgtgcacc ctctctggga gcagatcctg
    62701 cgcgtccgct tcagacgctg gcgcttccgt cacgcatggc cccggctccg gccgatcgtc
    62761 tgacgcgtga agagcgtaac agtctgctgt ggggagggat tgccacggtc agtgtggctg
    62821 ccggtgatgt ggttcagatc gaacgtcagg tgaccatgta tcgccagaac gtgtacggcg
    62881 aaagtgatcc cagctatctg gatgtggaaa ccatctacac cctctcctgg ctgcgttatt
    62941 ccctgcgtac ttttatcaca caacgttttc cgcgccataa actggctgat gacgggacgc
    63001 ccgttcgtgc cggtcagaat atcgtgacgc cggagatcat gaagcttcag ttaatttcgc
    63061 tgggtgaaga gtgggtggaa ctcgggctgg tcgaaaatct ggacaccttt aaaaagaatc
    63121 tgcttgtgga gcgtaatgcg tcagatcgaa accgtcttga cgtgttatgc acgccggatc
    63181 tggttaacca gttccgcttc ctggcagcac agatccgttt cattttgtga ggtaaggcat
    63241 gagcggaaaa cagtatcagg gtacggccac tatccgtgtg aacggtcagg aatacgagac
    63301 gcttgagggg gctacatttt ctccgtctgg ttttgaacgt gaagtggtga aaggcgcgaa
    63361 agtctatggt taccgccaga aaccacgtga agcgacgctg gactgcaaat ttccggcagg
    63421 cggagaaggt tcaccagccg ccgatgaaat caacaactgg actgcggtca ctatcgagtt
    63481 tgtggcggat accggcgaag tccacatgat gacgaaggcc tggagcagtg aaccggcctc
    63541 gcttgacggt ggcggggaga tctccgtgaa gttcgccagc gcctccagta cccgtgttca
    63601 gtgatcagga aaaataaaat gaccacacgt aagaaaaaaa cggcagtttc cgaggcagcc
    63661 gtgatggagg caatccggga agcacttgag ggggctgatc cacgcactgc cgggctgaca
    63721 gagcaactgg caaagggata tgtggatctg cttgatggtc tgccgtttgg tgagacccgt
    63781 gaatatcgtg tcacgttccg ggaactgaca gcgaaagaca gtattgacgc agaagcagaa
    63841 gccgagcgcg tggtggagac aaacaatggc ccgatgctga tagcgtctcc gtcgttacgc
    63901 ggtgttgcac tgctgcgccg tcagattgcc gcagtgggag acattgaagg tccgttgtca
    63961 ccccgtcaga ttggacagtt aagcgagcgc gatctctccc gcctgatggc agcggtcagc
    64021 ctgctggata ccgcgcttgc cggaaagctg gcagctgacc gggggcgatc aggcgcagtg
    64081 tcgggatcag attgaagaag cggcaattat cctggggatg gtgacaaaaa gcggtccgga
    64141 atgggcgctt aacctccccc tgtcgcaact ttaccggcac tgccgacaga cagaaaaaat
    64201 catcagaacg aagcagtaag tatggcccga aatctcagag catctctgat agttgatttg
    64261 ctcggcaata tctcagccaa atcccgccag tggtcacagg agctgggggc attctcacgc
    64321 tccggccagg cggggctggg tggcctggga aatgctgccc gccgtgccgg gcaggaaaca
    64381 gatgtactcg gaagcaggat gcagcgcacg ctggccgggg tgcggggcag tatccgccat
    64441 gtcacttctg attttgaccg tcttcagggc agtattaccg gcaccatcgg gcggatcagc
    64501 aatctttacg gaatgctggc cggtggtgct gctgtatatg gctttaaccg gggatttatt
    64561 cgcccggcag cggagatgga aacctatatg attcgcctga actcccttta caaaggggat
    64621 cgggcgaaaa ctgatgatgt ccgcaggtgg gcaatacaga atgcgaagga aaccacctgg
    64681 gggctggctg gcgtcatgca ggaatatacg tccagtctgg gctttggtat gagtgacagg
    64741 gaggcccgaa attttgtcac catgcttcag gatcagggtg ccgtgggcgg ctggtcactg
    64801 tccgatgcac agggggcatc cctgcaactg aaacagatgt atgcaagagg cagtattcag
    64861 gcggcggatg caaatattct tgccacctac ggcattaatg cctaccgcgt gctggctgat
    64921 cgtctgggtg tggaccagaa agtggtcaga aaacttggtg aaaaagggct gcttgggccg
    64981 gacagtattc gcctgttatt tcagacgctg gcggaacagg ccaggggagc acaaaaggac
    65041 gcaatgaatt cctggagtgg cctgacggcc atgatgggtg acgtctggga ccagtttgcc
    65101 cgggatgtga tggacagtgg gccatttgaa aaactgaagg gtaatctcag gggatttctt
    65161 tcatgggttg acagtgccaa atctgatggc agttaccatt ccctggcgga aagcacggca
    65221 tcagccatga accagggctt tgagtatgcc cgtgatgcag tgacagggtt ttatcaggcg
    65281 atcagaaagg tacgcgacac gcttcaggct ctgcgggatg cgggctatgg tgatgcgctg
    65341 gatcgcattg gtcagggggc acagaccgcc gcgaaatacc tgctgtacat gtatctcgcc
    65401 acccgtgccc tgaaaatggc gcgggctgtc ggaacgggga ttatacgtcc gggtgctgca
    65461 ttgctgggat acggattatc agcggcggcc ttcctgacct cacctttccg gcgctctcct
    65521 ccggccggaa caccaccagg cacatctcag ggacgagggc aacgttttat aaactttctt
    65581 accggggtaa atccggcagc cgttcagcca gtacttgtaa caaactggcc cacaggagga
    65641 ctgtccggca ccggcgcgac cgatacatcc tcaggcggac gtcaggggcg gggccgcagg
    65701 aaacgcggac cggggcgcgg aagaccggtc acgcctcccc tgccaccg
//
LOCUS       DAZFDP010000008        63625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFDP010000008 DAZFDP010000000
VERSION     DAZFDP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            327..4310
                     /gene="ehaA"
                     /locus_tag="ACHJ1G_000606"
     CDS             327..4310
                     /gene="ehaA"
                     /locus_tag="ACHJ1G_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4762511.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4452..5540
                     /gene="pdeL"
                     /locus_tag="ACHJ1G_000607"
     CDS             4452..5540
                     /gene="pdeL"
                     /locus_tag="ACHJ1G_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4762512.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5582..6514)
                     /gene="yahB"
                     /locus_tag="ACHJ1G_000608"
     CDS             complement(5582..6514)
                     /gene="yahB"
                     /locus_tag="ACHJ1G_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4762513.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(6606..7103)
                     /gene="yahC"
                     /locus_tag="ACHJ1G_000609"
     CDS             complement(6606..7103)
                     /gene="yahC"
                     /locus_tag="ACHJ1G_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4762514.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            7361..7966
                     /gene="yahD"
                     /locus_tag="ACHJ1G_000610"
     CDS             7361..7966
                     /gene="yahD"
                     /locus_tag="ACHJ1G_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4762515.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            8006..8869
                     /gene="yahE"
                     /locus_tag="ACHJ1G_000611"
     CDS             8006..8869
                     /gene="yahE"
                     /locus_tag="ACHJ1G_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4762516.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8859..10406
                     /gene="fdrA"
                     /locus_tag="ACHJ1G_000612"
     CDS             8859..10406
                     /gene="fdrA"
                     /locus_tag="ACHJ1G_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4762517.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10406..11824
                     /gene="yahG"
                     /locus_tag="ACHJ1G_000613"
     CDS             10406..11824
                     /gene="yahG"
                     /locus_tag="ACHJ1G_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4762518.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11967..12917
                     /gene="yahI"
                     /locus_tag="ACHJ1G_000614"
     CDS             11967..12917
                     /gene="yahI"
                     /locus_tag="ACHJ1G_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4762519.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            12927..14309
                     /gene="yahJ"
                     /locus_tag="ACHJ1G_000615"
     CDS             12927..14309
                     /gene="yahJ"
                     /locus_tag="ACHJ1G_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4762520.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            14608..15018
                     /locus_tag="ACHJ1G_000616"
     CDS             14608..15018
                     /locus_tag="ACHJ1G_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4762521.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            15272..16255
                     /locus_tag="ACHJ1G_000617"
     CDS             15272..16255
                     /locus_tag="ACHJ1G_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4762522.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            16304..17788
                     /locus_tag="ACHJ1G_000618"
     CDS             16304..17788
                     /locus_tag="ACHJ1G_000618"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4762523.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            17781..18752
                     /locus_tag="ACHJ1G_000619"
     CDS             17781..18752
                     /locus_tag="ACHJ1G_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4762524.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            18749..19705
                     /locus_tag="ACHJ1G_000620"
     CDS             18749..19705
                     /locus_tag="ACHJ1G_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4762525.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            19792..20841
                     /gene="yahK"
                     /locus_tag="ACHJ1G_000621"
     CDS             19792..20841
                     /gene="yahK"
                     /locus_tag="ACHJ1G_000621"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4762526.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            21084..21899
                     /gene="yahL"
                     /locus_tag="ACHJ1G_000622"
     CDS             21084..21899
                     /gene="yahL"
                     /locus_tag="ACHJ1G_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4762527.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            22311..22556
                     /gene="yahM"
                     /locus_tag="ACHJ1G_000623"
     CDS             22311..22556
                     /gene="yahM"
                     /locus_tag="ACHJ1G_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO4762528.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(22573..23244)
                     /gene="yahN"
                     /locus_tag="ACHJ1G_000624"
     CDS             complement(22573..23244)
                     /gene="yahN"
                     /locus_tag="ACHJ1G_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4762529.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            23391..23666
                     /gene="yahO"
                     /locus_tag="ACHJ1G_000625"
     CDS             23391..23666
                     /gene="yahO"
                     /locus_tag="ACHJ1G_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4762530.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(23764..25350)
                     /gene="prpR"
                     /locus_tag="ACHJ1G_000626"
     CDS             complement(23764..25350)
                     /gene="prpR"
                     /locus_tag="ACHJ1G_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4762531.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            25589..26479
                     /gene="prpB"
                     /locus_tag="ACHJ1G_000627"
     CDS             25589..26479
                     /gene="prpB"
                     /locus_tag="ACHJ1G_000627"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4762532.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            26524..27693
                     /gene="prpC"
                     /locus_tag="ACHJ1G_000628"
     CDS             26524..27693
                     /gene="prpC"
                     /locus_tag="ACHJ1G_000628"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4762533.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            27727..29178
                     /gene="prpD"
                     /locus_tag="ACHJ1G_000629"
     CDS             27727..29178
                     /gene="prpD"
                     /locus_tag="ACHJ1G_000629"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4762534.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            29218..31104
                     /gene="prpE"
                     /locus_tag="ACHJ1G_000630"
     CDS             29218..31104
                     /gene="prpE"
                     /locus_tag="ACHJ1G_000630"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4762535.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            31443..32702
                     /gene="codB"
                     /locus_tag="ACHJ1G_000631"
     CDS             31443..32702
                     /gene="codB"
                     /locus_tag="ACHJ1G_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4762536.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            32692..33975
                     /gene="codA"
                     /locus_tag="ACHJ1G_000632"
     CDS             32692..33975
                     /gene="codA"
                     /locus_tag="ACHJ1G_000632"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4762537.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(34015..34914)
                     /gene="cynR"
                     /locus_tag="ACHJ1G_000633"
     CDS             complement(34015..34914)
                     /gene="cynR"
                     /locus_tag="ACHJ1G_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4762538.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            35024..35683
                     /gene="cynT"
                     /locus_tag="ACHJ1G_000634"
     CDS             35024..35683
                     /gene="cynT"
                     /locus_tag="ACHJ1G_000634"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4762539.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            35714..36184
                     /gene="cynS"
                     /locus_tag="ACHJ1G_000635"
     CDS             35714..36184
                     /gene="cynS"
                     /locus_tag="ACHJ1G_000635"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4762540.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            36190..37371
                     /gene="cynX"
                     /locus_tag="ACHJ1G_000636"
     CDS             36190..37371
                     /gene="cynX"
                     /locus_tag="ACHJ1G_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4762541.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(37450..38703)
                     /gene="lacY"
                     /locus_tag="ACHJ1G_000637"
     CDS             complement(37450..38703)
                     /gene="lacY"
                     /locus_tag="ACHJ1G_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4762542.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(38755..41829)
                     /gene="lacZ"
                     /locus_tag="ACHJ1G_000638"
     CDS             complement(38755..41829)
                     /gene="lacZ"
                     /locus_tag="ACHJ1G_000638"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4762543.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(41952..43034)
                     /gene="lacI"
                     /locus_tag="ACHJ1G_000639"
     CDS             complement(41952..43034)
                     /gene="lacI"
                     /locus_tag="ACHJ1G_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4762544.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(43111..43944)
                     /gene="mhpR"
                     /locus_tag="ACHJ1G_000640"
     CDS             complement(43111..43944)
                     /gene="mhpR"
                     /locus_tag="ACHJ1G_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4762545.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            44135..45799
                     /gene="mhpA"
                     /locus_tag="ACHJ1G_000641"
     CDS             44135..45799
                     /gene="mhpA"
                     /locus_tag="ACHJ1G_000641"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4762546.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVTATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            45801..46745
                     /gene="mhpB"
                     /locus_tag="ACHJ1G_000642"
     CDS             45801..46745
                     /gene="mhpB"
                     /locus_tag="ACHJ1G_000642"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4762547.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            46763..47629
                     /gene="mhpC"
                     /locus_tag="ACHJ1G_000643"
     CDS             46763..47629
                     /gene="mhpC"
                     /locus_tag="ACHJ1G_000643"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4762548.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            47639..48448
                     /gene="mhpD"
                     /locus_tag="ACHJ1G_000644"
     CDS             47639..48448
                     /gene="mhpD"
                     /locus_tag="ACHJ1G_000644"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4762549.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            48445..49395
                     /gene="mhpF"
                     /locus_tag="ACHJ1G_000645"
     CDS             48445..49395
                     /gene="mhpF"
                     /locus_tag="ACHJ1G_000645"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4762550.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            49392..50405
                     /gene="mhpE"
                     /locus_tag="ACHJ1G_000646"
     CDS             49392..50405
                     /gene="mhpE"
                     /locus_tag="ACHJ1G_000646"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4762551.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            50680..51891
                     /gene="mhpT"
                     /locus_tag="ACHJ1G_000647"
     CDS             50680..51891
                     /gene="mhpT"
                     /locus_tag="ACHJ1G_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4762552.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            51993..52532
                     /gene="yaiL"
                     /locus_tag="ACHJ1G_000648"
     CDS             51993..52532
                     /gene="yaiL"
                     /locus_tag="ACHJ1G_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4762553.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(52657..53490)
                     /gene="fghA"
                     /locus_tag="ACHJ1G_000649"
     CDS             complement(52657..53490)
                     /gene="fghA"
                     /locus_tag="ACHJ1G_000649"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4762554.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(53583..54692)
                     /gene="frmA"
                     /locus_tag="ACHJ1G_000650"
     CDS             complement(53583..54692)
                     /gene="frmA"
                     /locus_tag="ACHJ1G_000650"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4762555.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(54727..55002)
                     /gene="frmR"
                     /locus_tag="ACHJ1G_000651"
     CDS             complement(54727..55002)
                     /gene="frmR"
                     /locus_tag="ACHJ1G_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4762556.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(55188..55961)
                     /gene="yaiO"
                     /locus_tag="ACHJ1G_000652"
     CDS             complement(55188..55961)
                     /gene="yaiO"
                     /locus_tag="ACHJ1G_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO4762557.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(55963..56544)
                     /locus_tag="ACHJ1G_000653"
     CDS             complement(55963..56544)
                     /locus_tag="ACHJ1G_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4762558.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            complement(56522..57718)
                     /gene="yaiP"
                     /locus_tag="ACHJ1G_000654"
     CDS             complement(56522..57718)
                     /gene="yaiP"
                     /locus_tag="ACHJ1G_000654"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO4762559.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(57728..58399)
                     /locus_tag="ACHJ1G_000655"
     CDS             complement(57728..58399)
                     /locus_tag="ACHJ1G_000655"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO4762560.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            59015..59977
                     /gene="tauA"
                     /locus_tag="ACHJ1G_000656"
     CDS             59015..59977
                     /gene="tauA"
                     /locus_tag="ACHJ1G_000656"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4762561.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            59990..60757
                     /gene="tauB"
                     /locus_tag="ACHJ1G_000657"
     CDS             59990..60757
                     /gene="tauB"
                     /locus_tag="ACHJ1G_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4762562.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            60754..61581
                     /gene="tauC"
                     /locus_tag="ACHJ1G_000658"
     CDS             60754..61581
                     /gene="tauC"
                     /locus_tag="ACHJ1G_000658"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4762563.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            61578..62429
                     /gene="tauD"
                     /locus_tag="ACHJ1G_000659"
     CDS             61578..62429
                     /gene="tauD"
                     /locus_tag="ACHJ1G_000659"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4762564.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(62535..63509)
                     /gene="hemB"
                     /locus_tag="ACHJ1G_000660"
     CDS             complement(62535..63509)
                     /gene="hemB"
                     /locus_tag="ACHJ1G_000660"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4762565.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT        15301 a        15841 c        17341 g        15142 t
ORIGIN      
        1 ccgcttttat cggctgtata ttaaatgtat tcctcttgct tttgtttata ttttgagttg
       61 atttgagttc ctggatacat ataatgcgca ggtattagag actgggagga atgtaatgat
      121 aaagttaaga gtgttaactt aaaataagtt ttaagtaata acttgctatt ttattaccct
      181 ctgaactaca tactcagata tgtggttgcc aatgctatgc aaaatttttg aatttttata
      241 tgcgatgaac agtaaccaga atggcattta atgcgttgct gtttatgcaa tcttggtttt
      301 atttggatgt attgctggag attgtgatga ataaaatata tcggctaaag tggaacaggt
      361 cccgtaactg ttggagcgtc tgctctgagc tggggagcag agtaaaagga aaaaagtccc
      421 gggctgtttt aattagcgcg ataagtttat attcatctct ggtattcgcc gatgatgtca
      481 tcgtaaacca ggataaaact attgattttg gcaaagagaa ccagagcatc gattaccgta
      541 ttacggtgac tgacaatgcc aatctggtaa tcaatacgac agatacttcc cgtccgcgtc
      601 tgactctcgc ttctggtggt gggttggata ttaccggagg aaaggtaaca atcaatggcc
      661 cgcttaactt tttgctgaaa ggtacggggt tcctgaatgt ctccaatgct ggcagcgagt
      721 tatatgctga tgatttgtat gagtcaaact caggcatgag acatgatcaa ggctatttta
      781 atgtctccaa tggcggcaaa attcatgtta agggcaccag tcgtctgact tatacgcagg
      841 ggaatgtaag cggcgaaggt agtcaggtaa atgcagggac cttctttatg ggcgtttacg
      901 gcggatatgg cggtaatcag ttcctgtcgg ttaataacgg tggtgaagtt aatgctcgtg
      961 aacatattag cctgggttat tacgaccaac gttctgatac aacattagtc gtatcagatg
     1021 gaggtaaaat atccgcaccg aaaattagtt taagcaccaa ctctgagtta gcgttaggtg
     1081 cacaggaagg aagtgctgcg aaggctgcag ggattattga tgccgaaaaa attgagtttg
     1141 tgtgggcaaa gacatccgat aagaaaatca ccttaaacca cacggataaa aacgcgacta
     1201 tttccgcgga tattgtcagt ggcagcgagg gcctgggcta tatcaatgcg ctcaatggca
     1261 cgacttactt aaccggtgat aactctgcct ttagtggtaa agtcaaaatt gagcaaaatg
     1321 gcgctttagg gatcaccaaa aatataggta cagcagagat caacaaccgc gggaaattac
     1381 acctgaaggc tgacgatagc atgacctttg ccaataagat ttccggcaat ggtacaataa
     1441 gtatcgacag tgggacagtg gcactgaccg gcaataacta tgcattcagc ggatatattg
     1501 atgtcgcttc tggtgctgtc gcggttattt ctgaagacaa gaacatcggc cgtgcggacc
     1561 tggatgttga tggcaaactg caaatcaatg ccaataaaga ttgggtattt gataacgatc
     1621 ttcaaggtag aggtattgtt gaaataaaca tggggaatca cgaattctcc ttcgatgagt
     1681 ttgcttatac agactggttc cagggttcac tggcgttcca gaacacgaca tttaacctgg
     1741 aaaagaatgc tgagtttctg cagagaggcg ggatcactgc gggtcaggga agccaggtaa
     1801 cagtgggtaa gggcgctcac tccattagca ctttgggatt ctccggcgga accgttgatt
     1861 ttggtgccct gacagcaggt gcacagatga cagaagggac ggtcaacgtt agtaaaacgc
     1921 tggatttgcg cggcgagggt gtgattcagg tttctgacag tgacgttgtc cgctcagtat
     1981 ctcgtgatat tgactctgcg ttatcgctca ctgaagtcga tgatggtaac agcgccatta
     2041 agctggttga tgcgcaaggt gcggaagttc tgggcgatgc gggcaatctg caattgcagg
     2101 ataaaaatgg gcaaatcctc tccagcagcg cccaacgtga tattcagcag aatgggcaaa
     2161 aagcggccgt cggcacttac gactatcgtc tgacgagtgg ggtaaacaat gacggtctgt
     2221 atatcggtta cggcctgacc cagcttgatt tacacgctac cgacagcgat gctctggtgc
     2281 tgagctctaa cggtaaaagc gagaatgccg ccgatctcag cgcaaagatt accggcagtg
     2341 gtgacctggc attcagcagc cagaagggtc agaccgtatc gctttctaac aaagacaacg
     2401 actataccgg tgttaccgat ctgcgcagtg ggacgctttt gttgaataac gataacgtgt
     2461 tgggtaatac ccatgaactg cgtctggcgg cagagactga actggatatg aatggtcaca
     2521 gccagaccgt gggcacgctc aatggcagcg ccgattcact gctgagctta aatggcggca
     2581 gtctgacggt taccaacggg ggcacttcaa ccggttcgtt aacggggagc ggagagctga
     2641 atattcaggg cggcacgctg gacatcgcgg gcgataacag caacctgacg gcgaatgtga
     2701 acattgctaa ttcggctaat gtcctggtaa gtcatgcgca gggattgggt agcgcaaacg
     2761 ttgagaacaa cggtaccctg gcgttgaata atagcactga aaaaagagcg gctgcgtctg
     2821 tgaattacac cctgggcggc aatctgacca acaacggtac gctgatgacc ggaatgtcag
     2881 gacagcaagc tggcaatgtg ttagtggtga aggggaacta ccacggtaat aacggtcaac
     2941 tagtaatgaa tacggtactg aatggcgatg actcagtaac cgataaattg gttgtcgagg
     3001 gcgatactag cggcacgact gccgttacgg tgaataacgc tggcggtaca ggtgcgaaaa
     3061 cccttaacgg tatcgaactt atccatgtag acggtaagtc tgagggcgaa tttgttcagg
     3121 ctggtcgtat cgttgcgggg gcgtatgact acactctcgc gcgtggacaa ggggcaaata
     3181 gtggtaactg gtatctgacc agcggcagtg attctcctga actgcagccg gagccagacc
     3241 cgatgccgaa tccggagcca aacccgaatc cagagccgaa ccctaacccg acacctacgc
     3301 cgggtccgga tctgaatgtg gataatgacc tgcgaccgga ggcgggtagc tacattgcga
     3361 accttgcagc agcgaataca atgttcacca cgcgtctgca tgagcgtctg ggtaatacgt
     3421 actataccga catggtgacg ggtgagcaga aacaaaccac tatgtggatg cgccatgaag
     3481 gtggtcataa taaatggcgt gatggcagcg gccagctgaa aacccaaagc aatcgctatg
     3541 ttctgcaact gggaggcgat gtcgcgcagt ggagccaaaa cggcagcgac agctggcatg
     3601 ttggggtcat ggcgggatat ggcaacagcg acagcaaaac catttcctcg cgaaccggtt
     3661 atcgtgcaaa agcgagtgtg aacggatata gcaccggcct ctatgccacc tggtatgccg
     3721 atgacgagtc gcgtaatggc gcgtatctcg acagttgggc gcagtacagc tggtttgata
     3781 acacagtgaa aggggatgac ttacaaagtg aatcctataa atcaaaagga tttaccgctt
     3841 cactggaagc tggatacaaa cacaaattag ctgaatttaa tggcagccag ggaacgcgta
     3901 atgaatggta tgttcagccg caagcacagg ttacctggat gggagtcaaa gccgataagc
     3961 accgcgaaag caacggaacc ctcgttcata gcaacggtga tggcaatgtt caaacccgac
     4021 ttggcgtaaa aacctggctg aagagccacc ataaaatgga tgacggtaaa tcccgcgagt
     4081 tccagccgtt tgtagaagtg aactggctgc ataacagtaa ggatttcagc accagtatgg
     4141 atggcgtgtc cgtcactcag gatggagccc gaaatattgc tgagataaaa accggggtgg
     4201 aaggacagct aaatgccaac ctgaatgtct gggggaatgt gggcgttcag gttgccgata
     4261 ggggatataa tgacacctct gcaatggttg gcattaagtg gcaattctga tctgaataat
     4321 gagtaaaagg agagagagcc gaacgggaga taattcggct ctctgtgtca ttttcttgca
     4381 gccagcttta tcagtcagag tgaagactcg cagatttagc tggcgcacaa gatttaatca
     4441 aggtgtgttc aatggcttcg catgatttga gtgttttttt ggaagagttc ggggcaacgg
     4501 tgaatttgac gttgcctggt atcgtgtccg aaaaagaacg gcttttactc aagctgctaa
     4561 tggagggaat gtcggttaca gaaatctcac agtacagaaa tcgcagtgca aagacaattt
     4621 cacatcaaaa gaagcagctg tatgaaaaac taggcatcca gagtgatatt accttctggc
     4681 gggatatctt ttttcaatat catccgcaag tcatcagtgg cacgggaaat aaaaataatt
     4741 tttatatccc tgataaccgt tgtcaccata tcgtcacgcc tgaagccatc agtctggcgt
     4801 tggaaaacca cgaattcaaa ccgtggatcc aaccggtttt ctgcgcgcag actggggtac
     4861 tgacgggctg tgaggtactt gtccgctggg aacatccaca aacgggaatt atcccaccgg
     4921 atcagtttat tcctctggcg gagtcatccg gtcttattgt cataatgacc cgccaactga
     4981 tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggac aatttccata
     5041 ttggcatcaa cgtctcggcg ggttgttttt tggcagcggg atttgaaaaa gagtgtctga
     5101 acctggttaa gaaattaggt aacgataaaa tcaagctggt tctcgagcta acggaacgta
     5161 accctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat cagcacaaca
     5221 ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttac ttgcaggcgt
     5281 tcccggtcga ttttattaag atcgataagt catttgtgca aatggcgagt gtcgacgaaa
     5341 tctccggtca tattgtggac aatattgtcg aactagcgcg taagcctggt ctgagtatcg
     5401 tggcggaagg ggtagaaacc caggagcagg cggatttaat gatcggtaaa ggcgttcact
     5461 ttttgcaggg ctatttgtac tctccgccag taccgggtaa taaatttatc tctgaatggg
     5521 taatgaaagc aggtggttga tgtaaaccgc tattcacagc gcatcgggag gttggcagcg
     5581 attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca tttggcgcaa
     5641 attgtttttt aatccactgc gtcacctgac ccgtagcaga atgctgagtc gccagtaaca
     5701 tgcgcgaatc ctggcgcgga ttatggattt gtcgggtaac cagcagggat tgcgtcatcg
     5761 cctcacggac catgtaatcc ggcaaaaaac caattccttc acctaaaatc tggcactgac
     5821 atttggtgtt gaaatctggc accagaattg actcctgccc gtgcagcaac cagcccactt
     5881 ttttattaat cgtatgcgcg gtgtcctcca ccataatatt agggtagaga cgcagttggc
     5941 tttcggcgat gggttccggc acaaaggcta acgggtgatc cggggcgata gcaaaagccc
     6001 agcgaatcgc gccaatttca gtgtaatcaa taccgccgcc gtccagtagt gtatcgggtg
     6061 cgccgatggc gatattggcc tgattattaa taatcgcatc ccagacgccg ttatacactt
     6121 cggtggtgac ggtaatctgg caggtaggaa actgcttttt cagcacctgt aacaagcgtg
     6181 cggtgtgttt gggcgtataa agcagctgat taatacaaat gcgcacacgc gcttctatgc
     6241 cctgcgaaat agtatcgata ctgcgtttga tggcataaaa atcattcagc aggtcggtag
     6301 ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta ctgcgagtga
     6361 acagcaccac atccagcccc gtctccatac gcttgatggt gtagctaatg gcggaagtgg
     6421 ttaaacccaa ctcttctgcg gctttactga agctgccaaa acgcgcagcg gtagtaaatg
     6481 ccaacagatt ctcttcggta aaaattgagt tcattaaacc attctcgcca ttatctacta
     6541 tgaatttctc cagctatgac tgtagaggta tcggttaaag atagataatc atttttgaat
     6601 aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc accatattct
     6661 gaaattatcc ccacgcagtt acccgccaga aaacccagta acgtcaccgt ccagttcatc
     6721 ccgctggcaa aaaacagcgt catgccgaga aagccaccgg gaataaagga cagtaaccag
     6781 aaacgccctt gccagaccac taccgccgag aagggtacgg tagtaattac gctcgcccag
     6841 aacgcggaca gtccacttac agaggccagc caaccggcag ccagcgcggc gaaaaatgcc
     6901 cacaccatcc cggaataatt aaccgccaga cttctgataa accccgattt accgccgcct
     6961 gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc ccatccctga
     7021 gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc gacggtgaca
     7081 cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac gaaagctact
     7141 cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta tcgggtcaaa
     7201 gaaacaaata ttgcactacc gcacactgcg aaaagattgt tgaacatcat tcaacaaaaa
     7261 ggcgaaatac agcgaattaa atttaagcgg gttcttttat cagattatcg ttgctattct
     7321 caattcatcg aaaacagcca caaaaaccag gggaatgtga atgagtataa aaaatctacc
     7381 tgccgactat ttattggctg cacaacaggg tgatatcgat aaagtaaaaa cctgccttgc
     7441 gctgggtgtc gatattaata cctgcgatcg tcaggggaaa acggcaatta cgctggcaag
     7501 tttatatcag caatatgctt gcgttcaggc attaattgat gccggagcgg atattaataa
     7561 gcaagatcat acctgtttaa atcctttttt aattagctgt ctgaacgatg atttaacgct
     7621 actacgaatt attttaccgg ctaaacccga tcttaattgc gtaacccgtt ttggcggtgt
     7681 cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc ttttggcgca
     7741 tacggagatt aacgttaacc agaccaacca tgtaggctgg acgccgctac tggaagcgat
     7801 tgtgcttaat gatggtggta ttaaacagca ggcgattgtg cagttattgc tggaacacgg
     7861 tgccagcccg catctgaccg ataaatatgg caaaacgcca ctggaactgg cgcgggaaca
     7921 gggctttgaa gagattgcgc agttactgat tgccgcaggt gcataaaccg ggaggcttgc
     7981 tatcaacaca ccagaaagac ggtgtgtgtg ggcgctaact gcggatgcgg attttctggc
     8041 gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata tcgcactccc
     8101 ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc caaacagttg
     8161 tcggttggca ctcactcact ttgatgatct gttccggcat ggtgataagg ttcagtttga
     8221 cgatcaaggt attacggttg gtcaacatct tcatatagag atgagtcgtt gtcggcgttg
     8281 gctgtcccca accttgcaaa tgaccgctgt gaattttcac cttatcgcct ggctacagtg
     8341 gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt atcgcggcga
     8401 taatccgttt tatcaggcgt taaataaaga actacatatt aaacgacggg cagttattca
     8461 ggcagtaaac gaaaaacaaa atatcgccgc agcggtcgcc agtatgattg ggttagggat
     8521 tggccttacg ccatcagccg acgattattt aacaggtctg gcgcttattt tatttattcc
     8581 cgggcatccg tcggaaaaat acaaagagga attttatctc ggtatgcagc gcggcagaaa
     8641 taacaccaca ttattaagtg ctataacgct ggaagccgca ttacaacaac gttgccggga
     8701 aaatattcat cgttttattc acaacattat ttatgacatc cctgggaacg caactcaggc
     8761 aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt atggcatggc
     8821 cgatggttgt gcgctgagcc aaacctacgg agggaattat gtcagttaaa atagtcatta
     8881 aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac
     8941 tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaacaaa ggtgtactga
     9001 aaaatttagg actgctgacg ccggagctgg aacaggcgaa aaacggcgac ctgatgattg
     9061 tcatcaatgg taaatcgggt gcggacaacg agcagttgct ggtggagatt gaagaactgt
     9121 tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta cgccactatt gccagcgcca
     9181 aaaagcatat cccggaaagt aacctggcgg tgatttcggt caacggtctg tttgccgctc
     9241 gcgaagcgcg tcaggcgctg caaaacgatc tcaacgtgat gctgttttcc gataacgtct
     9301 cagttgaaga tgaactggcg ctcaagcagt tggcccacga aaaagggctg ctgatgatgg
     9361 ggccggactg cggcacggcg attattaacg gcgcggcgct ctgttttggt aacgccgtgc
     9421 gtcgcggcaa tatcggtatt gttggcgcat ccggcaccgg cagccaggag ctgagcgtcc
     9481 gcattcatga atttggtggc ggtgtttcac aactgattgg caccggcggg cgcgacctga
     9541 gcgagaaaat cggcggcctg atgatgctcg acgccatcgg gatgctggaa aacgatccgc
     9601 aaactgaaat tatcgcactg atttcaaaac ctcccgcgcc tgcggtggcc cgcaaagtgc
     9661 tggaacgcgc acgtgcctgc cgcaagccgg tggtggtctg cttcctcggt cgcgttgaaa
     9721 cgccggtgga tgagcagggg ctacagtttg cccgcggcag caaagaggcg gcgctaaaag
     9781 cggtgatgct ctccggcgtg aaacaggaaa atctcgacct gcatacgctt aaccagccgt
     9841 tgattgctga tgtgcgtgcg cgtctgcaac cgcagcagaa atacattcgt ggcctgttct
     9901 gcggcggcac gctgtgcgac gaaaccatgt tcgcggtgat ggaaaaacat ggcgatgtct
     9961 acagcaacat tcagcccgat ccggaattcc gcctgcaaga tatcaaccgc agcatcaaac
    10021 acaccttcct cgactttggc gatgacgact tcacgaacgg caagccgcat ccaatgattg
    10081 accccaccaa ccgcatcagt cgcttgatcg aagaggcgcg cgatccagaa gtggcggtga
    10141 tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggc tccaccatcg
    10201 aggcgatcaa agaagcgaaa gcgatcgccg ctgccgaagg acgcgagttg atcattctcg
    10261 cctatgtgct gggtaccgat cttgatacgc catcgttaga acaacaaagc cagatgctgc
    10321 ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg gcgcgtgaat
    10381 ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac cattgaacgt
    10441 tattaacgtc ggcatcgcca tgtttagcga tgacctgaaa aagcagcatg tagaagtgac
    10501 tcaactcgac tggacgccgc cggggcaggg caatatgcag gtggtgcagg cgctggataa
    10561 cattgccgat tcgccgctgg cagacaaaat cgccgccgct aaccagcagg cgctggagcg
    10621 cattatccag tcgcatccgg tgctgattgg ttttgatcag gcgattaacg tggtgccggg
    10681 catgacgccg aaaaccattc ttcacgccgg gccgccgatc acctgggaaa aaatgtgcgg
    10741 cgcgatgaaa ggcgcggtca ccggggcgtt ggtgttcgaa gggctggcga aagatctcga
    10801 cgaggcggct gaactggcgg cctccgggga gatcaccttc tcgccgtgcc acgaacacga
    10861 ctgtgtggga tcgatggcgg gtgttacctc tgcctcgatg tttatgcaca tcgtaaaaaa
    10921 caaaacctac ggcaacatcg cttacaccaa tatgagcgag cagatggcga agattttgcg
    10981 tatgggagcc aacgatcaga gcgtgatcga ccgcctcaac tggatgcgcg atgtgcaggg
    11041 gccaatgctg cgcgacgcga tgaaaattat cggcgagatt gatctgcgct taatgctggc
    11101 gcaggcgctg catatgggcg atgagtgcca taaccgcaat aacgccggga cgacgttgct
    11161 gattcaggcg ctgacgccgg gaattattca ggcaggttat tccgtggaga aacagcgcga
    11221 agtgtttgag tttgtcgcta gcagcgacta cttctccggc ccgacgtgga tggcgatgtg
    11281 taaagcggcg atggatgcgg cgcacggcat cgaatacagc accgtggtca ccaccatggc
    11341 gcgtaacggc gtcgagttcg gcctgcgggt cagcggatta ccggggcaat ggtttaccgg
    11401 tccggcgcag caggtcatcg gcccgatgtt tgccggttat aagccggaag attcggggct
    11461 ggatatcggc gacagcgcca tcactgaaac ctacggtatt ggcggatttg ctatggcgac
    11521 cgcgcctgca atcgttgcgc tggtgggcgg cacggtggaa gaggctatcg atttctctcg
    11581 ccagatgcgt gaaatcactc tcggtgaaaa ccccaacgtc accattccgc tgctcggttt
    11641 tatgggcgtg ccgtcggcaa tcgacatcac ccgcgtgggt agcagcggca ttctgccggt
    11701 gatcaacact gccatcgccc ataaagatgc gggcgtcggc atgattggcg cgggcattgt
    11761 gcatccacct tttgcctgct tcgagaaagc cattcttggc tggtgcgaac gttacggcgt
    11821 ctgacctaca tgttcatgcc ggatgcggcg tgaacgcctt atccggccta cggatggccc
    11881 gagaattgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt cataacctaa
    11941 tcaccctctg tagcggacat aacaacatga aagagcttgt ggtcgttgcc attggtggca
    12001 acagcattat caaagataac gccagccagt cgattgagca tcaggcggag gcggtgaaag
    12061 ccgtggccga tacggtgttg gaaatgctgg cttccgatta cgacattgtg ctgacccacg
    12121 gtaacgggcc gcaggtcggg ctggatttac gccgtgcgga gattgcccac gagcgcgaag
    12181 ggctgccctt aacgccgctg gcgaactgtg tggcagatac gcagggcggc atcggctatc
    12241 tgatccaaca ggcgctgaat aaccggctgg cgcgtcacgg cgagaaaaaa gccgtcaccg
    12301 tggtaactca ggtggaagtg gataaaaacg atccaggttt tgcccatccc accaagccca
    12361 ttggtgcatt cttcagtgaa agccagcgtg acaaactaca aaaggcaaac cctgactggt
    12421 gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg gaaccgaaac
    12481 gtattgtcga agcacccgcc attaaggcgc tgatccaaca aggttttgtc gtaattggcg
    12541 cgggcggcgg tggaattccg gtagtgcgta ctgaagcggg ggattaccaa agcgtggacg
    12601 cggttattga caaagatctc tctaccgcgc tgctggcccg tgaaattcac gccgacattc
    12661 tggtgatcac caccggcgtc gaaaaagtct gtatccactt tggtaaaccg cagcagcagg
    12721 cgctggatcg ggtggatatt gccaccatga ctcgctatat gcaggaaggg cattttccgc
    12781 ccggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggagcag ggcggcaaag
    12841 aagtgattat caccacgccg gaatgcctgc ctgcggcgct gcgcggtgaa acgggcaccc
    12901 atattattaa aacgtaagga cgtaggatga aagaaagcaa tagccgccgt gaatttctga
    12961 gccagagcgg taagatggtc accgccgccg cgctgtttgg tacctctgtg ccgctcgccc
    13021 atgcggcggt agctggcacc ctaaactgcg aagcgaacaa caccatgaaa atcactgacc
    13081 cgcattacta tctcgataac gtgctgctgg aaaccggttt tgactacgaa aacggtgtgg
    13141 cggtgcagac ccgcacggcg cgccagaccg tggagattca ggacggcaaa attgtcgccc
    13201 tgcgcgagaa caagcagcat ccggacgcca cgctgccgca ctatgacgct ggcggtaagt
    13261 tgatgctacc caccactcgc gacatgcata ttcatctcga caaaaccttc tacggcgggc
    13321 cgtggcgctc gctcaatcgt ccggcaggca ccactatcca ggacatgatc aagcttgagc
    13381 agaaaatgct gccggaactg caaccgtaca cgcaggaacg ggcagaaaaa ctgatcgatc
    13441 tattgcagtc gaaaggcacc accattgccc gcagccactg caatatcgaa ccggtttccg
    13501 gcctgaaaaa tctgcagaat ttgcaggcgg tgctggcgcg acgtcaggcg ggcttcgagt
    13561 gtgaaatcgt cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa cccttaatgc
    13621 gcgaagcgat gcaggcgggt gcgcattacg tcggcgggct ggacccgacc agtgtcgatg
    13681 gcgcgatgga aaaatccctc gacaccatgt ttcagattgc gctggactac gacaaaggtg
    13741 tcgatattca cctgcacgaa accactccgt cgggcgtggc agccatcaat tatatggttg
    13801 aaacggtaga gaaaacgccg caactgaaag gtaagctgac catcagccac gcctttgcgc
    13861 tggctacgct caacgaacaa caggtcgatg cgctggcgaa tcggatggcg gcgcagcaaa
    13921 tttctatcgc ctcgacggtg ccgattggca cgctgcatat gccgctcaaa cagttgcacg
    13981 acaaaggcgt aaaagtgatg actggcactg acagcgttat cgaccactgg tcgccttatg
    14041 gtctgggcga catgctggaa aaagccaatc tctacgcgca gctctatatt cgtcctaacg
    14101 aacagaacct ctcccgttcg ctgtttctag ccactggcga tgtattgccg ctgaatgaaa
    14161 aaggcgagcg tgtatggcca aaagcgcagg atgatgccag ctttgtgctg gtggacgcct
    14221 cctgttccgc cgaggcggtg gcgcgtatct cgccgagaac cgcaacgttc cataaagggc
    14281 aactggtgtg ggggagtgtg gcaggttgat gcgcatttga aacgcctgca acggtgaatt
    14341 gtaagagatt taagccccag ggagtggggc gggaatggtt tgctaatgaa tgatcctgaa
    14401 ctttataatc tcgcggcata atttccaggg gattatttga gagaaataca caaaagtgag
    14461 ttttaacaaa ggtatttatc aggccataaa tgtgacctgc ttcggataaa aaagattcag
    14521 tatcgaaaaa ccaatgatac tcttgctgat ggttaccaac agtaattaag atgtgatttg
    14581 tttttccgta atcgcaaaaa tattgccatg accataaaag aaaaattctc acaaaaatat
    14641 ccccatgctt ccttttgtac ttttggcgat tcagcagcgt tggccgatca cctcgcaacg
    14701 ttgattgcga ctggtgttaa aacagcttcc tgtggttcgc tggcgggatg tattgaagac
    14761 aacgcatttc cgatgattgg tgagtataag attgtagaaa atagccgtgg tgagcctgta
    14821 tgtgttatca gagtgattgg cctgcattta ctgcgctttt ctgatgtcac cgtggagctt
    14881 gcccgtaagg aaggtgaagg tgacctcagt cttgaatatt ggcgtaatga acaccgtcgt
    14941 tttttccagg cagaaggaag ctattcacca gaaatggacg ttatatttga agaatatgcc
    15001 cttattgacg ttgtgtagat aactatttat ctacgcgaaa taaactcaca cacataaaat
    15061 atcgctaatt ttgtttattt tgctattttt tacgtgatga atgtcacact tattgttgct
    15121 gcccgttttg tccatattta taaccatatt tttaatagct gcctgaaata gggctgttta
    15181 ttctgcataa aaatattcga aaattcttat tctctccagc gcgctaattt gggcgaacac
    15241 ttcctgacta ccctgcaatg aggctgaaat gatgaataaa cgttttgtta tcaatatggt
    15301 gtcttcgctg ttattaggcg ctgcgctaat ttctgcgccg ctccaggcag cggaaaaagt
    15361 cgtggtgaat atctctaaag tcgatggtat gccgtggttt aatcgcatgg gcgaaggtgt
    15421 ggttgaggcg ggtaacgcct ttggtgttaa tgcgtcacag gtggggcctt caagtactga
    15481 tgctccgcaa caagttaaaa ttattgagga tttaatcgcc cgtaaggtta atgcaattac
    15541 tatcgtgcca aacgatgcca acgtgctgga accggtattc aaaaaagcgc gagatgccgg
    15601 aattgtggtt ctgaccaacg aatcccccgg tcagccaagt gctaactggg atatcgaaat
    15661 cattgataac gaaaaatttg ccgctgaata tgttgagcat atggcgaaac gcatgggggg
    15721 caagggcggc tatgtgattt acgtgggtag tttgaccgtg ccgcagcata acttatgggc
    15781 tgacttgctg gtgaaatacc agaaagagca ttacccggat atgcatgaag ttacccgccg
    15841 catgccggtt gccgagagtg tggatgactc acgtcgtacc acactggacc tgatgaaaac
    15901 ctatcctgac ttgaaagcgg ttgtctcctt tggttcaaac ggtccgattg gcgcgggacg
    15961 tgcggtgaaa gagaaacggg cgaaaaataa agtcgcagtc tatggcatga tgatcccttc
    16021 acaggcggca tcactgatta aaagtggtga tatcaccgaa gggattactt acgattcggc
    16081 ctctgcaggt tacgcactcg cagccgtcgc cagcacgttg ctgaagggtg aagagatcaa
    16141 acctggatta gaaatgcaga atctgggtaa ggccgacgta gatatggata agcgaattat
    16201 ccgtttccat aaggtattac tggtcaacaa agacaatatc gactcacttt attaatccat
    16261 ttcttcgggg cgaaagcccc gattcttatt gccaggggat gttatggaaa ccttcctttc
    16321 ccttcgtcat atcaacaaaa cgtttcatgc cacgcgagcg ctgcgcgatg tttctctcga
    16381 ctttatgtcg ggtgaagtgc actgccttgc cgggcaaaac ggctgtggta aatcgacgtt
    16441 gattaaaatt atgtccggtg tttatcgccc ggatgaggga gcggaaatta cgcttggtgg
    16501 gaaaaactgg tcaaagctga cacccgccgc ttcggtggcg caggggattc aggtgattta
    16561 tcaggacctc tctttatttc ctaacctgag tgtctgggag aatatcgccg tgaatcacta
    16621 tcaccacggt ctgtttgtta accgccgtcg tctgcgtgag gtggcgcagg cggcaatgac
    16681 cagtattaac gtcacattgc cactggatac gctcgtttct gagctttcaa ttgcccgctg
    16741 tcagctggta gcaatttgcc gtgcactggc gcaggacgca cggttgatcg tgatggatga
    16801 accaaccgct tcgctgaccc atcaggaagt gcaagggtta ttacaggtgg tgcatcaatt
    16861 gcgggaacgc gggatctgcg tggtcttcgt cagtcatcgt ctggaagaag tgatggaagt
    16921 ttccgaccgt atttcagtgc tgaaagatgg tgagctggtc gggacttttc cggctgcaga
    16981 gatgaccaca aaacaactcg gtttcctgat gaccggccag gagttcgaat atcaggtgcg
    17041 agagttgtgg cagggaaaat ccagcacccc ggtgcttgag gtgcgtaatt taagtcgtca
    17101 tggggaatat ctaaatatta acttgcgggt ggaagcgggc gaagtggtat cgattgttgg
    17161 cctgcttggt gcggggcgca cggaattatg tctgagtctg tttggtatga cccgaccgga
    17221 tgctggcgag atcctcatca acggccagcc ggttacactg catagtaacc aggacgctat
    17281 ccgccacggt attggttatg tttcggaaga tcgcatgtcg cgcggtttgg tgatggcgca
    17341 atctattgaa gacaacatca ttagcactgt ttttcacaag gtaaaagatc gcttcggctt
    17401 tctgagtgaa actaaagttc gtgacctggt ggacaggcta atcaaggcat tgaccatcaa
    17461 agtctctgat cctcatcttc cggtgaatac gctttctggt ggtaacgcgc agcgagtatc
    17521 gatcgccaaa tggctggcga ttgggccacg actgttgatt ctcgatagcc cgacagtcgg
    17581 tgttgatatc gccaacaaag caggaatata cggcattatc agcgatctgg cggctcatgg
    17641 cattgcggta ttgatgatct gcgatgaaat tgaagaagcg tggtatcaaa gccaccggat
    17701 tttggtaatg caaaaaggtc agatcaccca tagcttcctg cctgacagca gttctcaggc
    17761 caggattgcg gaggtggtaa atggctgaac tgaaaaaacg ccatgaattc tggctggcat
    17821 tactgattgt ggtgctgttt gttggccttg cctggcgcag cgacgagttt ctgacattcg
    17881 gtaatttgta cgatctcgcc aataactatg ccatgttgac tattctcgcc tgtggtttgt
    17941 ttgtggtgtt gatttccggt ggaattgata tttcgtttcc agcaatgacc atcattgcgc
    18001 aatacggcat ggtgctgttg ctgcaaaaaa ttggtggcaa cttcgctgtc gcgtttgcac
    18061 tggcgggctg catcggcatt ttacttggct taattaacgc cttactggtt aatcgcctac
    18121 gggtgccttc tatcatcatc actatctcga cgctgaatat tttctatggc ctgctgttat
    18181 ggttgagtaa aggtgtgtgg ctgtacgact ttccgccgtg gtttgagcag ggggttatgt
    18241 tgttcaagta caccgatgct gatggctatg actatggcct tggtctgccg ctgatcgcca
    18301 tgattacggt ggtgctgctg acagcgttta tcatgaattt caccagtgta gggcgcaaaa
    18361 tttatgccct tggcgggaac cgcgaatcag ccagtcgcat cggctttagc gtgctgaaac
    18421 tgcaactttt cgtctacggc tatatgggat tgatgtctgg cgctgcgggt gtagtgcagt
    18481 cgtggacggt gatgactgtc gcccccgatt ctcttctggg ttatgagctg acagtactgg
    18541 ctgcggtggt gcttggcggc actagtttgc tcggcgggcg cggcacgtta accggtactt
    18601 tgctcggcgt ggtgttgttg gcagtgatgc aaaacgggct aaatttattg ggagtctcgt
    18661 cttactggca aacattgatc accggcatca tcatcgttgc cagcattagt gccacggcgt
    18721 ggagtcagca tcagaaccgg agtctgctat gaaaaaatcg tggcgtaata acgttgagtt
    18781 ttacttgatc ggcctgctgg tgctaactgt tgccgcgttc agcattacta tgccggagat
    18841 tttctggtcg ataagtaatt tccagtccgt cgcttcgcaa atgcccgtgc tgggcattct
    18901 ggcgttggct atggcggtga ccatgctttg cggtggcatt aacttgtcaa ttatagccac
    18961 tgctaacgcc tgttcactgg tgatggcgtg ggtggcgact cagtatccac cgggtattgc
    19021 tacggttgtc gctacgttac tggcgggcgc tggcgcggcg gtgataatcg gtttatgtaa
    19081 cggcgtattg attgctggta ttcgagtgtc gccaattctt gcaacgcttg ggatgatgac
    19141 gttgctcaaa gggataaata ttctggtgac aggtggtagc gccattgcca attatccctc
    19201 atgggtgctg tggttgaatc atgcgcaatg gttcggtatt ccgctaccga tgtggttgtt
    19261 tgccgcagtg gcactggggc tgtggatatt gctggagaaa acgccgctcg gtaaagccat
    19321 ttacctgatt gggtctaacg aacgagcaac actttactcc ggcattaaca ctcgtcgggt
    19381 gctgatttgg gtttatgtca tttccgcctt gctttgtgcg gtagcggcat ttttgatgat
    19441 gtcgaaactc aactcggcta aggcctctta tggcgaatcg tacttgctgg tgtcaatcct
    19501 cgcggcagta ctcggcgggg ttaatccgga cggcggcagc gggcggataa ttggtatggt
    19561 gctggcgctg tttcttctgc aaattattga aagcggcttc aatattctcg gtataagccc
    19621 ctacctgacg atggccttat ggggaacgtt gctgctctgt ttcattcagg ttcgcggcat
    19681 gttggggctg gatcgggtgg tttaaatatt ggctatattc aatggacgcg ttttgccgcg
    19741 atgacatatc aggcatcgcc aaatacacat agctaatcag gagtaaacac aatgaagatc
    19801 aaagctgttg gtgcatattc cgctaaacaa ccacttgaac cgatggatat cacacggcgt
    19861 gaaccgggac cgaatgatgt caaaatcgaa atcgcttact gtggcgtctg ccattccgat
    19921 ctccaccagg tccgttccga gtgggcgggg acggtttacc cctgcgtacc gggtcatgaa
    19981 attgtggggc gtgtggtagc cgttggtgat caggtagaaa aacatgcgcc gggcgatctg
    20041 gtcggtgtcg gctgcattgt cgacagttgt aaacattgcg aagagtgtga agacgggctg
    20101 gaaaactact gtgatcacat gaccggcacc tataactcgc cgacgccgga cgaaccgggc
    20161 catactctgg gcggctactc acaacagatc gtcgttcatg agcgatatgt tctgcgtatt
    20221 cgtcacccgc aagagcagct ggcggcggtg gctcctttgt tgtgtgcagg gatcaccacg
    20281 tattcgccgc tacgtcactg gcaggccggg ccgggtaaaa aagtgggcgt ggtcggcatc
    20341 ggcggcctgg gacatatggg gattaagctg gcccacgcga tgggggcaca tgtggtggca
    20401 tttaccactt ctgaggcaaa acgcgaagcg gcaaaagccc tgggggccga tgaagttgtt
    20461 aactcacgca atgccgatga gatggcggct catgtgaaga gtttcgattt cattttgaat
    20521 acagtagctg cgccacataa tctcgacgat tttaccacct tgctgaagcg tgatggcacc
    20581 atgacgctgg ttggtgcgcc tgcgacaccg cataaatcgc cggaagtttt caacctgatc
    20641 atgaaacgcc gtgcgatagc cggttctatg attggcggca ttccagaaac tcaggagatg
    20701 ctcgattttt gcgccgaaca tggcatcgtg gctgatatag agatgattcg ggccgatcaa
    20761 attaatgaag cctatgagcg aatgctgcgt ggtgatgtga aatatcgttt tgttatcgat
    20821 aatcgcacac taacagactg aaaaaattaa taaatacgct gtggtttaac atattaactt
    20881 cgctctccac ttaacttttt agttaaggag agcgaaataa tatcaaagta gcagtaaaac
    20941 ctataacgta aattcaaatt gttaaattaa ctccctccag tacacaatac ttcacacgtt
    21001 agttatgagc gatttctgat agtgcctggt ttaatgagag ctttattatc tgcgacgttt
    21061 atttttattt aagagagatc gcgatgatat cattaaaagc tccgcacaat aatttgatgc
    21121 catatacgca acaaagcata cttaatacgg ttaaaaacaa tcagttacca gaggatatca
    21181 aaagctccct ggtttcctgt gtcgatatat tcaaggtttt gattaaacaa tattatgatt
    21241 atccttatga ttgtcgtgat gatttagtcg atgacgacaa actcattcat ctcatggctg
    21301 ccgtacgaga ctgtgagtgg tcagacgata acgcactcac cataaatgtg cagtttaatg
    21361 atttccctgg attttatgac tggatggatt accctgatca cccggttaag tttgtttttc
    21421 gcatacttga aaatcaaaaa gggacagttt gggtatatga ccaggatgat gcgtttcttg
    21481 atattaaagc aaatgtgcag gctggacgct ttaccgggct taagaaatta gtgcagttta
    21541 tcgattcagt gcgaacagat tgcaaatgta ttcttcttga acaccatatg cctttgctga
    21601 gaattttccc taaagggaaa gagtgtatgc gtgtggaaaa atggcttcgc gaaatgtcgt
    21661 ctattccgga gacagacgcg cccattaagc aggcgcttgc gcatggatta ttattacact
    21721 taaaaaatat ccaccctgtt tttcctgaat cactggtgat gttattgctc tctgttctgg
    21781 acgtaaaaac atacagagat gacgcacgat taaatgaatg gatatcgaat agagtgcagg
    21841 aattaggtga cagatattat cctgtaaata agcacgttaa aatccgctac actctttagt
    21901 tcagaaaggg ttccacctgc aagttgttat tccaccagag agtgaatcgc aaagcaggta
    21961 atcatttcat ctgtatatcc gtatgccaaa tggcattcca ccgccttttt cgccgatatt
    22021 ttgttgtgat cggcgacgct tcgcttaaaa aagcaccagc agtggtttcg cagctatgcg
    22081 gtgtataaaa aatgatctca tgcagatgtt ttgtgaatgt gtgggttgac attcatataa
    22141 aaaaaatcat aattccatca tgtttgtgtg gggtcttttc tgtaccttag catcgcactc
    22201 aagcctgaca gaaaatatgc tgtaaggctc atatcaaaaa ccgccattag ctcatcagga
    22261 agagcagacg acaaccagga ttgttgtatg gtacggggtt cgaggcctcg atggcggtcc
    22321 aacttttcaa aactcttctg aatcagatcc ccttattatc atccctgcaa agtggaacat
    22381 tgccattgtt tggctattca ggacgggtca gaccaatgaa aaaagcccac acaggggaga
    22441 gtgggctgaa atgggaagct aaagactcaa gtaaacttat cggaaataag gaccacgcat
    22501 tacgggggca atcatcgccg atggtcggga ttcggcaaat caggttgatt acgtagagag
    22561 taaattatct gctcaccgct gcgtcacccc ttcgtaaatc aggcgtagcg cgaatacacc
    22621 aataattgca ccaataaccc gactggcaac gcgttgcata cgcccataag cacgacgcac
    22681 agcgggcaaa gaaaacgcct gactaagaaa aactcgccag ataattgatg cgagcacaat
    22741 ccccgcccag gccattaaac gtgcccatgt tggtgtttcg gcatttaatg ttactgagaa
    22801 aatactgata aaaaataaaa cggtttgcgg gttagagaga tcggtaatta atccgcggcg
    22861 aaaaaagaca taccaggggg cgctaatcgg ttgttgtagt gtgctcattt gcggtgttga
    22921 ctggcggcgc atgctgcacc acgcaaacca taagagataa gcgccgccga cgattctgat
    22981 aagcgaaaaa atctcctcac actgcgtaat tagcgttgca agaccaaaca aacccaaccc
    23041 ggaataaaat gcatcgccca gcgccacgcc cagcccggtc agcacccctg cgcgtcgacc
    23101 ggaagccagg ctggtttgta ctaccacaaa gagattggct cccggattaa aaaaagtaat
    23161 cacgaacagt cctacggtca ggtaaacggc gtgcaaagga tccatagtga tttcatccat
    23221 aaataagtga agtaacttca tcatatttct accaaaaata atcgggtgcg agagagatca
    23281 caaagtgtct tatttccggt tactggcgtt tatgccctga ctgaactaat tattaatcaa
    23341 cccaataatg tgggtggatg atagtgtgat aacaactctg gagccgtaat atgaaaataa
    23401 tctctaaaat gttagtcggt gcgttagcgt tagccgttac caatgtctat gccgctgaat
    23461 tgatgaccaa agcggaattt gaaaaagttg aatcgcagta tgaaaaaata ggtgatattt
    23521 caactagcaa tgaaatgtcg actgcagacg cgaaagaaga tttgatcaaa aaagcggatg
    23581 aaaaaggggc tgatgtgttg gtactgacct ccggtcaaac tgacaataag atccacggca
    23641 cggcaaatat ttataagaag aagtaattct gaaccctatg taaacatgcc cgatgcgtaa
    23701 gtttatcggt gatcatctat tgaaatttat gccggataaa gcgttcgcgc tgcattcggc
    23761 agttcagctt ttcagccgcc gccagaacgt cgtccggctg atgcctaaat aattcgccgc
    23821 tgctgtttta tcgccattaa atttctccag tgcctgttgt ggtgtcagta agcgtggagc
    23881 gggagttttc gccgactcgc gcgccagttc cggcagtagc agctgcaaaa attgcggcgt
    23941 taaatccggc gtcggttcca cacttaaaaa tagcgccagt cgctccatca tattgcgcag
    24001 ttcacgaata ttgcccggcc agtcgtagtg caccagcacg gtttcgcttg cctgtaatcc
    24061 caggcgtaat gcggcagaaa acggggcgga gagcgccgcc agtgacactt tcaaaaagct
    24121 ttccgccagc ggcagaatat ccgccacccg ctcacgcagt ggtggcagtt gcaggcgtaa
    24181 aatgctcagc cgataaaaca ggtcacggcg aaactgccct tgccgcatat cttcttccag
    24241 cttgcagtga gtggcgctaa tgacccgcac atccaccgga acaggctgat gcccgccgac
    24301 gcgggtaacc tctttttctt ccagcacccg cagcagccgg gtctgcaaag gtagcggcat
    24361 ttcgccaatc tcatccaaaa acagcgtgcc accgtgggcg atttcaaaca gcccggcgcg
    24421 acctccgcgt cgcgagccgg taaacgcccc ttcctcatag ccaaacagct ccgcttccag
    24481 cagcgattcg gcaatcgccc cgcagttgac ggcaacaaac ggatgcgact ttttgccctg
    24541 tcgcgcatcg tggcgggcaa agtattcccg atgaattgcc tgggctgcca gttctttgcc
    24601 cgtccccgtt tccccctcaa tcaacaccgc cgcactggag cgggcataca gcaaaatagt
    24661 ctgccgcacc tgttccatct gtggtgattg accgagcata tcgcccagca cgtaacgagt
    24721 acgcagggcg ttgcgagtgg catcgtgagt gttatggcgt aacgacatgc gcgtcatatc
    24781 cagcgcatcg ctgaacgcct ggcgcacggt ggcggcggaa tagataaaaa ttccggtcat
    24841 tccggcttct tctgccagat cggtaatcag ccccgcgccg accaccgctt cggtgccgtt
    24901 agcttttagc tcgttaatct gcccgcgtgc gtcttcttcg gtaatgtagc tacgttgatc
    24961 gaggcgcaaa ttaaaggttt tttgaaacgc caccagtgcc ggaatagttt cctgataagt
    25021 gacaacgccg atagaagagg tgagttttcc ggcttttgcc agtgcctgta acacatcgta
    25081 gccgctcggt ttaatcaaaa taactggcac tgacaggcgg cttttcaggt acgcgccgtt
    25141 agatccagcc gcgatgatgg cgtcacagcg ttcgtttgcc agtttcttgt ggatgtaggt
    25201 caccgctttt tcaaagccaa gctggatagg ggtaatgttc gccaggtgat caaactcgag
    25261 gctgatatcg cgaaacagct cgaacaggcg cgttacagat accgtccaga taaccggttt
    25321 gtcgtcatta agccgtggtg gatgtgccat agcgcaccgc aaagttaaga aaccgaatat
    25381 tgggtttagt cttgtttcat aattgttgca atgaaacgcg gtgaaacatt gcctgaaacg
    25441 ttaactgaaa cgcatatttg cggattagtt catgacttta tctctaacaa attgaaatta
    25501 aacatttaat tttattaagg caattgtggc acaccccttg ctttgtcttt atcaacgcaa
    25561 ataacaagtt gataacaaag gatgggctat gtctctacac tctccaggta aagcgtttcg
    25621 cgctgcactg actaaagaaa atccattgca gattgttggc accatcaacg ctaatcatgc
    25681 gctgttggcg cagcgtgccg gatatcaggc aatttatctt tctggcggtg gcgtggcggc
    25741 aggttcgctg gggctgcccg atctcggtat ttctactctt gatgacgtgc tgaccgatat
    25801 tcgccgtatc accgacgttt gttcgctgcc gctgctggtg gatgcggata tcggttttgg
    25861 ttcttcggcc tttaacgtgg cgcgcaccgt gaaatcgatg attaaagccg gtgcggcagg
    25921 attgcatatt gaagatcagg ttggtgcgaa acgctgcggt catcgtccga ataaagcgat
    25981 cgtctcgaaa gaagagatgg tggatcggat ccgcgcggcg gtggatgcga aaaccgatcc
    26041 tgattttgtg atcatggcgc gcaccgatgc tctggcggta gaggggctgg atgcggcgat
    26101 cgagcgtgcg caggcctatg ttgaagcggg tgccgagatg ttgttcccgg aggcgattac
    26161 cgaactcgcc atgtaccgcc agtttgccga tgcggtgcag gtgccaatcc tcgccaacat
    26221 taccgaattt ggtgccacgc cgctgtttac catcgacgaa ttacgcagcg cccatgtcgc
    26281 aatggcgctg tacccgcttt cagcgttccg cgccatgaac cgcgccgctg agcatgtcta
    26341 caacgtcctg cgtcaggaag gcacgcagaa aagcgtcatc gataccatgc aaacccgcaa
    26401 cgagctgtac gaaagcatca actactacca gtacgaagcg aagctcgacg acctgtttgc
    26461 ccgtagccag gtgaaataaa acacaacgaa aaatatctcg accctacaaa tgacgaggac
    26521 aacatgagcg acacaacgat cctgcaaaac agtacccatg tcattaaacc gaaaaaatct
    26581 gtggcacttt ctggcgttcc ggcgggcaat acggcgctct gcaccgtggg taaaagtggc
    26641 aatgacctgc attaccgtgg ctacgatatt cttgatctgg cggaacattg cgaatttgaa
    26701 gaagtggcgc acctgctgat ccacggcaaa ctgccgaccc gtgacgaact cgccgcttac
    26761 aaaacgaaac tgaaagccct gcgcggttta ccggctaacg tgcgtaccgt gctggaagcc
    26821 ttaccggcgg cgtcgtaccc gatggatgtc atgcgcaccg gcgtttccgc gctcggctgc
    26881 acactgccag aaaaagaggg gcacactgtc tctggcgcgc gggatattgc cgacaaactg
    26941 ctggcatcac ttagctcgat tctcctttat tggtatcact acagtcacaa cggcgaacgc
    27001 atccagccgg aaaccgatga cgactccatc ggtggtcact tcctgcatct gctgcacggc
    27061 gaaaagccat cgcaaagctg ggaaaaggcg atgcatattt cgctagtgct gtacgccgaa
    27121 cacgagttta acgcctccac ctttaccagt cgggtgattg cgggcactgg ctctgatatg
    27181 tattccgcga ttattggcgc gattggcgca ctgcgcgggc cgaaacacgg cggagcgaat
    27241 gaagtgtcgc tggagatcca gcaacgctac gaaacgccgg acgaagccga agccgatatc
    27301 cgcaagcggg tggaaaacaa agaagtggtc attggttttg gtcatccggt ttataccatc
    27361 gccgacccgc gccaccaggt gatcaaacgt gtggcgaagc agctctcgca ggaaggcggc
    27421 tcgttgaaga tgtacaacat cgccaatcgc ctggaaacgg tgatgtggga gagcaaaaag
    27481 atgttcccca atctcgactg gttctctgct gtttcctaca acatgatggg cgttcccacc
    27541 gagatgttca caccactgtt tgtcatcgcc cgtgtcaccg gctgggcggc gcacatcatt
    27601 gaacaacgtc aggacaacaa aattatccgt ccttccgcca attatgttgg accggaagac
    27661 cgccagtttg tcgcgctgga taagcgccag taaacctcta cgaataacaa taaggaaaca
    27721 tacccaatgt cagctcaaat caacaacatc cgcccggaat ttgatcgtga aatcgttgat
    27781 atcgtcgatt acgtgatgaa ctacgaaatc agctctaaag tggcctacga caccgcacat
    27841 tactgcctgc tcgacacgct cggctgcggt ctggaagctc tcgaataccc ggcctgtaaa
    27901 aaactgctgg ggccaattgt tcctggcacc gtcgtaccca acggcgtgcg cgtccccgga
    27961 actcagtttc agctcgaccc tgtccaggcg gcatttaaca ttggcgcgat gatccgctgg
    28021 ctcgatttca acgatacctg gctggcggcg gagtgggggc atccttccga caacctcggc
    28081 ggcattctgg caacggcaga ctggctttcg cgcaacgctg tcgccagcgg caaagcgccg
    28141 ttgaccatga aacaggtgct gaccgcaatg atcaaagccc atgaaattca gggctgcatc
    28201 gcgctggaaa actcctttaa ccgcgtcggc ctcgaccacg ttctgttagt gaaagtggct
    28261 tccaccgccg tggtcgccga aatgctcggc ctgacccgcg aggaaattct caacgccgtt
    28321 tcgctggcgt gggtggacgg tcagtcgctg cgcacctatc gccatgcgcc gaacaccggc
    28381 acgcgtaaat cctgggcggc gggcgatgcc acttcccgcg cggtacgtct ggcactgatg
    28441 gcgaaaacgg gcgaaatggg ttacccgtca gccctgaccg cgccggtatg gggcttctac
    28501 gacgtctcct ttaaaggtga atcgttccgc ttccagcgtc cgtacggttc ttacgtcatg
    28561 gagaatgtgc tgttcaaaat ctccttcccg gcggagttcc actcccagac ggcagttgaa
    28621 gcggcgatga cgctctatga acagatgcag gcagcaggca agacggcggc ggatatcgaa
    28681 aaagtgacca ttcgcaccca cgaagcctgt attcgcatca tcgacaaaaa ggggccgctc
    28741 aataacccgg ctgaccgcga ccactgcatt cagtacatgg tggcgatccc gctgttattc
    28801 gggcgcttaa cggcggcaga ttacgaggac aacgttgcgc aagataaacg catcgacgcc
    28861 ctgcgcgaga agatcaattg ctttgaagat ccggcattta ccgctgacta ccacgacccg
    28921 gaaaaacgcg ccatcgccaa tgccattacc cttgagttca ccgacggcac acgatttgaa
    28981 gaagtggtgg tggagtaccc cattggtcat gctcgccgcc gtcaggatgg tattccgaaa
    29041 ctggtcgata aattcaaaat caatctcgcg cgccagttcc cgactcgcca acagcagcgc
    29101 attctggagg tttctctcga cagaactcgc ctggaacaga tgccggtcaa tgagtatctc
    29161 gacctgtacg tcatttaagt aaactgcggt aaggcgtaag ttcaacagga gagcattatg
    29221 tcttttagcg aattttatca gcgttcgatt aacgaaccgg agcagttctg ggccgagcag
    29281 gcccggcgta ttgactggca gacgcccttt acgcaaacgc tcgaccacag caatccgccg
    29341 tttgcccgtt ggttttgtga aggccgaacc aatttgtgcc acaacgctat cgaccgctgg
    29401 ctggagaaac agccagaggc actggcattg attgccgtct cttcggaaac agaagaagag
    29461 cgcaccttta cctttcgcca gctgcatgac gaagtgaacg cggtggcctc aatgttgcgc
    29521 tcactgggcg tgcagcgcgg cgatcgggtg ctggtgtata tgccgatgat tgccgaagcg
    29581 catattactc tgctggcctg cgcgcgcatt ggtgctattc attcggtggt atttggtggt
    29641 tttgcctcgc acagcgtggc ggcgcgaatt gatgacgcta aaccggtgct gattgtctcg
    29701 gctgatgccg gagcgcgcgg tggcaaaatc attccctata aaaaattgct cgacgatgcg
    29761 ataagtcagg cgcagcacca gccacgccat gttttgctgg tggatcgcgg gctggcgaaa
    29821 atggcgcgcg tcagcgggcg ggatgtcgat ttcgcgtcgt tgcgccatca acacatcggc
    29881 gcgcgggtac cggtggcgtg gctggaatcc aacgaaacct cctgcattct ctacacttcc
    29941 ggcacgaccg gcaaacctaa aggcgtgcag cgtgacgtcg gcggatatgc ggtggcgctg
    30001 gcgacctcga tggacaccat ttttggcggc aaagcgggca gcgtgttctt ttgcgcatcg
    30061 gatatcggct gggtggtggg gcattcgtat atcgtttacg cgccgctgct ggcggggatg
    30121 gcgactatcg tttacgaagg attgccgacc tggccggact gcggcgtgtg gtggaaaatc
    30181 gtcgagaaat atcaggttag ccggatgttc tcagcgccga ccgccattcg cgtgctgaaa
    30241 aaattcccta ccgctgaaat tcgcaaacac gatctctcgt cgctggaagt gctctatctg
    30301 gctggagaac cgctggacga gccgaccgcc agttgggtga gcaatacgct ggatgtgccg
    30361 gtcatcgaca actactggca gaccgaatcc ggctggccga ttatggcgat tgctcgcggt
    30421 ctggacgaca ggccgacgcg tctgggaagc cccggcgtgc cgatgtatgg ctataacgtg
    30481 cagttgctta atgaagtcac cggcgaaccg tgtggcgtca acgagaaagg gatgctggtg
    30541 gtggaagggc cgctgccgcc gggctgtatt cagaccatct ggggcgacga cgaccgcttt
    30601 gtgaagactt actggtcgct gttttcccgc ccggtgtacg ccacctttga ctggggcatc
    30661 cgtgacgctg acggttatca ctttattctc gggcgcactg acgatgtaat taacgttgcc
    30721 gggcatcgtc tgggcacgcg cgagattgaa gagagtatct ccagccatcc gggcgttgcc
    30781 gaagtggcgg tggttggggt gaaagatgcg ctgaaagggc aggtggcggt ggcgtttgtc
    30841 attccgaaag agagcgatag tctggaagat cgtgatgtgg cgcattcgca agagaaggcg
    30901 attatggcgc tggtggacag ccagattggc aactttggtc gcccggcgca cgtctggttt
    30961 gtctcgcaat tgccgaaaac gcgatccgga aaaatgctgc gccgcacgat ccaggcaatt
    31021 tgcgaaggac gcgatcctgg ggatctgacg accattgatg atcctgcgtc attggatcag
    31081 atccgccagg cgatggaaga gtaaggtatt gtcggatgcg tcgcgtggcg catccgacac
    31141 catgcgccga tgcctgatgc gacgcttacc gcgtcttatt atgcctacag gcctgaaccg
    31201 taggtcggat aaggcgctcg cgtcgcatcc gacaccccgc gccgatgcct gatgcgacgc
    31261 tgacgcgtct tatcagtatc tacccactac ccactgtttt tacaccgata atttttcccc
    31321 cacctttttg cactcattca tataaaaaat atatttcccc acgaaaacga ttgcttttta
    31381 tcttcagatg aatagaatac ggcggatttt ttgggtttca aacagcaaaa agggggaatt
    31441 tcgtgtcgca agataacaac tttagccagg ggccagtccc gcagtcggcg cggaaagggg
    31501 tattggcatt gacgttcgtc atgctgggat taaccttctt ttccgccagt atgtggaccg
    31561 gcggcactct cggaaccggt cttagctatc atgatttctt cctcgcagtt ctcatcggta
    31621 atcttctcct cggtatttac acttcatttc tcggttacat tggcgcaaaa accggcctga
    31681 ccactcatct tcttgctcgc ttctcgtttg gtgttaaagg ctcatggctg ccttcactgc
    31741 tactgggcgg aactcaggtt ggctggtttg gcgtcggtgt ggcgatgttt gccattccgg
    31801 tgggtaaggc aaccgggctg gatattaatt tgctgattgc cgtttccggt ttactgatga
    31861 ccgtcaccgt cttttttggc atttcggcgc tgacggttct ttcggtgatt gcggttccgg
    31921 ctatcgcctg cctgggcggt tattccgtgt ggctggctgt taacggcatg ggcggcctgg
    31981 acgcattaaa agcggtcgtt cccgcacaac cgttagattt caatgtcgcg ctggcgctgg
    32041 ttgtggggtc atttatcagt gcgggtacgc tcaccgctga ctttgtccgg tttggtcgca
    32101 atgccaaact ggcggtgctg gtggcgatgg tggccttttt cctcggcaac tcgttgatgt
    32161 ttattttcgg tgcagcgggc gctgcggcac tgggcatggc ggatatctct gatgtgatga
    32221 ttgctcaggg cctgctgctg cctgcgattg tggtgctggg gctgaatatc tggaccacca
    32281 acgataacgc actctatgcg tcgggtttag gtttcgccaa cattaccggt atgtcgagca
    32341 aaaccctttc ggtaatcaac ggtattatcg gtacggtctg cgcattatgg ctgtataaca
    32401 attttgtcgg ctggttgacc ttcctttcgg cagctattcc tccagtaggg ggcgtgatca
    32461 tcgccgacta tctgatgaac cgtcgccgct atgagcactt tgcgaccacg cgtatgatga
    32521 gtgtcaattg ggtggcgatt ctggcggtcg cgctggggat tgccgcaggc cactggttac
    32581 cgggaattgt tccggtcaac gcggtattag gtggcgcgct gagctatctg atccttaacc
    32641 cgattttgaa tcgtaaaacg acagcagcaa tgacgcatgt ggaggttaac agtgtcgaat
    32701 aacgctttac aaacaattat taacgcccgg ttaccaggca aagaggggct gtggcagatt
    32761 catctgcagg acggaaaaat cagcgccatt gatgcgcaat ccggcgtgat gcccataact
    32821 gaaaacagcc tggatgccga acaaggttta gttataccgc cgtttgtgga gccgcatatt
    32881 cacctggaca ccacgcaaac cgccggacaa ccgaactgga atcagtccgg cacgctgttt
    32941 gaaggcattg aacgctgggc cgagcgcaaa gcgttattaa cccatgacga tgtgaaacaa
    33001 cgcgcctggc aaacgctgaa atggcagatt gccaacggca ttcagcatgt gcgtacccat
    33061 gtcgatgttt cggatgcaac gctaactgcg ctgaaagcaa tgctggaagt gaagcaggaa
    33121 gtcgcgccgt ggattgatct gcaaattgtc gccttccctc aggaagggat tttgtcgtat
    33181 cccaacggtg aagcgttgct ggaagaggcg ttacgcttag gggcagatgt agtgggggcg
    33241 attccgcatt ttgaatttac ccgtgaatac ggcgtggagt cgctgcataa aaccttcgcc
    33301 ctggcgcaaa aatacgaccg tctcatcgac gttcactgtg atgagatcga tgacgagcag
    33361 tcgcgctttg tcgaaaccgt tgctgccctg gcgcaccgtg aaggcatggg cgcgcgagtc
    33421 accgccagcc acaccacggc aatgcactcc tataacgggg cgtatacctc acgcctgttc
    33481 cgcttgctga aaatgtccgg tattaacttt gtcgccaacc cgctggtcaa tattcatctg
    33541 caaggacgtt tcgatacgta tccaaaacgt cgcggcatca cgcgcgttaa agagatgctg
    33601 gagtccggca ttaacgtctg ctttggtcac gatgatgtct tcgatccgtg gtatccgctg
    33661 ggaacggcga atatgctgca agtgctgcat atggggctgc atgtttgcca gttgatgggc
    33721 tacgggcaga ttaacgatgg cctgaattta atcacccacc acagtgccag aacattgaat
    33781 ttgcaggatt acggcattgc cgccggaaac agcgcaaacc tgattatcct gccggctgaa
    33841 aatggatttg atgcgctgcg ccgtcaggtt ccggtacgtt attcggtacg tggcggcaag
    33901 gtgattgcca gtacacaacc ggcacaaacc accgtatatc tggagcagcc agaagccatc
    33961 gattacaaac ggtaatttcc tctgtgttga agagccactg acctgtgtca gtggctaccg
    34021 tgattcattc tcgccaacat ccgcacattc ctccaacgcc atgtgcaaaa atgccttcgc
    34081 ggcggctgtc tgccagctat ttttccgccg caacaaaacc gccgttctct ccagtagtgg
    34141 cggggcaaga gaaatcgctt taagcccgtc atgttgtgtg gcaatcgctg ctggtaacaa
    34201 tgtggaaagg gaagtgcggc gaatcagctc cagaaccgcg ctaattgagt tcgcctcaat
    34261 gaccacctgt ggatgtagcc ccgctttctc gcagtagtgg tcaatttgct ctctggtggc
    34321 aaattccgcg ctgagcagga ccagtttttc atcatgcaag cgactcaacg ccacctgttc
    34381 acaggcagcc agcggatgat gttgcgccac gactaacgct aaactttctg tcagtaaagg
    34441 aattgcctcc agctccggcg aatgcacagg cgcgaaggca atcccaacgt ccagctcgtc
    34501 gcggcaaagc agatcttcga ttttctcctg cgacatttcc tgtagctgga gcgtgatgcc
    34561 ggggtagcgc gcatagaaat ccgccattaa agggccgata aagtagcttg taaaggtggg
    34621 ggtgacggcg atacgcagcg atcctcgcgt cagatcggca acatcatgaa tcgcccgttt
    34681 acccgctccc agttcctgta acgcccggct ggcgtactgt cgccagactt ctcctgcatc
    34741 ggtgagacga atcgttcgcc cgctacggtc aaacagcggc acgcctaaac tctcctctaa
    34801 ctggcgaatc tgctgggaaa gcgcaggctg agagacgtgc aacgcactgg cggcacgggt
    34861 gaagctgcca tgttcagcca cggcaagaaa ataattgata tgtcgagaga gcattcgcca
    34921 acctataagt aaatccaata gaactcatca taaatgagac ttttacctta tgacaatcgg
    34981 cgagtagtct gcctctcatt ccagagacag acagaggtta acggtgaaag agattattga
    35041 tggattcctt aaattccagc gcgaggcatt tccgaagcgg gaagccttgt ttaaacagct
    35101 ggcgacacag caaagcccgc gcacactttt tatctcctgc tccgacagcc gtctggtccc
    35161 tgagctggtg acgcaacgtg agcctggcga tctgttcgtt attcgcaacg cgggcaatat
    35221 cgtcccttcc tacgggccgg aacccggtgg cgtttctgct tcggtggagt atgccgtcgc
    35281 tgcgcttcgg gtatctgaca ttgtgatttg tggtcattcc aactgtggcg cgatgaccgc
    35341 cattgccagc tgtcagtgca tggaccatat gcctgccgtc tcccactggc tgcgttatgc
    35401 cgattcagcc cgcgtcgtta atgaggcgcg cccgcattcc gatttaccgt caaaagctgc
    35461 ggcgatggta cgtgaaaacg tcattgctca gttggctaat ttgcaaactc atccatcggt
    35521 gcgcctggcg ctcgaagagg ggcggatcgc cctgcacggc tgggtctacg acattgaaag
    35581 cggcagcatc gcagcttttg acggcgcaac ccgccagttt gtgccactgg ccgctaatcc
    35641 tcgcgtttgt gccataccgc tacgccaacc gaccgcagcg taaccttatt tttaaaccat
    35701 caggagatcc accatgattc agtcacaaat taaccgcaat attcgtcttg atcttgccga
    35761 tgccattttg ctcagcaaag ctaaaaaaga tctctcattt gccgagattg ccgacggtac
    35821 cggtctggca gaagccttcg taaccgcggc tttgctgggg cagcaagcgc ttcctgccga
    35881 cgccgcccgc ctggtcgggg cgaagctgga tctcgacgaa gacgccattc tactgttgca
    35941 gatgattcca ctgcgtggct gcattgatga ccgtattcca accgacccaa cgatgtatcg
    36001 tttctatgaa atgttgcagg tgtacggcac aaccctgaaa gcgttggttc atgagaagtt
    36061 tggcgatggc attatcagcg cgattaactt caaactcgac gttaagaaag tggcggaccc
    36121 ggaaggtggc gaacgtgcgg tcattacctt agatggtaaa tatctgccga ccaagccgtt
    36181 ctgacagcca tgcgcaatca tcaaaagacg ttcacgatgc tgctggttct ggtgctgatt
    36241 ggtcttaata tgcgaccact gctcacctcc gtcgggccac tgctaccgca attgcgccag
    36301 gcgagcggaa tgagctttag cgtggctgcc ctgttgaccg ctcttccggt ggtcaccatg
    36361 ggcgggctgg cgctggccgg aagctggctt catcagcatg tcagcgaacg tcgcagtgtc
    36421 gccatcagtc tgttgctgat tgccgtcggt gcattgatgc gtgagcttta cccccaaagt
    36481 gtgctgctgc ttagcagcgc actgcttggt ggggtgggga tcggtatcat tcaggcggtg
    36541 atgccttcag tgattaaacg gcggtttcag caacgcacgc cactggtgat ggggctgtgg
    36601 tctgcggctc tgatgggcgg cggtggactt ggtgccgcca taactccctg gttagttcaa
    36661 catagcgaaa cctggtatca aacactcgcc tggtgggcgc tgcctgccgt tgttgcgctc
    36721 tttgcctggt ggtggcaaag cgcccgcgag gtcgcctctt cccacaagac aacaaccact
    36781 ccggttcgcg tggtattcac tccccgcgcg tggacgctgg gtgtttactt cggtctgatt
    36841 aacggcggtt acgccagcct gattgcctgg ttacccgctt tctatattga gattggtgcc
    36901 agcgcgcagt acagcggttc cttactggca ttgatgacgc ttgggcaagc cgcaggagct
    36961 ttgctgatgc ctgctatggc tcgccatcag gatcggcgca aactgttaat gctggcgctg
    37021 gtgttacaac tggtggggtt ctgcggcttt atctggttgc cgctgcaatt gccggtatcg
    37081 tgggcgatgg tgtgtgggtt aggtctgggc ggcgcgtttc cgctctgttt gctgctggcg
    37141 ctcgatcact ctgtgcaacc ggctattgct ggcaagctgg tggcgtttat gcaggggatc
    37201 ggttttatca tcgccgggct tgccccgtgg ttttctggcg tgctgcgtag tatcagcggc
    37261 aattacctga tggactgggc gtttcatgcg ctgtgcgtcg ttgggctgat gatcataacc
    37321 ctgcgttttg caccagcacg ttttccgcag ctgtgggtca aagaggtatg atgcgacgct
    37381 tgtttcgcca gatgatggag ctataaataa ttgtcggatg tgcccagtgg cgcatccgac
    37441 attaattgct taagcgactt cattcacctg acgacgcagc agggaaagcg ggccggggcc
    37501 gctaagcgtg aacacggaaa ttaaggtgaa gcccagcgcc accagaccca gcaccagata
    37561 agcgccctgg aaaccgatgc tttcatacat attgcccgcc agtacagaca taaaaatcat
    37621 cgccagttgc ttaaagaagc agaaacagac cagataaatc gtcgctgaaa aacgcacttc
    37681 aaactggctg gtaatatatt taaagcagcc caccagcagg aacggtactt caaacatatg
    37741 cagcgttttc agaataacca cttccagcgc tgaggtggcg aacgatgagc caataatacg
    37801 tacagacata atagtgccag ccagcagcag ggcgtttttc ccaccgatgc gattaatgat
    37861 cagtggcgca aagaacataa tcgaggcgtt aagtaattcg cccattgtcg ttacgtagcc
    37921 aaatacccgc gtaccctgtt caccggtagc aaagaacgaa gtaaagaaat tagcaaactg
    37981 ttggtcaaaa acatcgtagg tgcaggaaac gccaataaca tacagtgaca aaaaccacag
    38041 ttttggctgt ctgaacagtt ccagtgccag cttaaggcta aatgccgaat ggttggcacc
    38101 taccgcattg gcaaccgtgg cggaagaggg cgcatccgtt ttggcgaaaa agagtaaaac
    38161 ggcgaggatg agtgcacagc cagaacccag ccagaaaaca aactgattat tgatggtgaa
    38221 catgatgccg acaatcgagg cacacaacgc ccagccaaca cagccaaaca tccgcgcgcg
    38281 accaaattcg aaattactgc gacggctgac tttctcaata aatgcctcta ctgctggcgc
    38341 accggcgtta aaacaaaagc ctagataaat accaccaaca atcgatccta ctaaaatgtt
    38401 gtattgtaac agtggcccga agataaaaat aaagaacggc gcaaacatca ctaacatgcc
    38461 ggtaataatc cacagcaggt atttgcgcag cccgagtttg tcagaaagca gaccaaacag
    38521 cggttggaat aatagcgaga acagagaaat agcggcaaaa ataatacccg tatcactttt
    38581 gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac gggaagtagg ctcccatgat
    38641 aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag tttgtgtttt ttaaatagta
    38701 cataatggat ttccttacgc gaaatacggg cagacatggc ctgcccggtt attattattt
    38761 ttgacaccag accaactggt aatggtagcg accggcgctc agctggaatt ccgccgacac
    38821 tgacgggctc caggagtcgt cgccaccaat ccccatatgg aaaccgtcga tattcagcca
    38881 tgtgccttct tccgcgtgca gcagatggcg atggctggtt tccatcagtt gctgttgact
    38941 gtagcggctg atgttgaact ggaagtcgcc gcgccactgg tgtgggccat aattcaattc
    39001 gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg tacggggtat acatgtctga
    39061 caatggcaga tcccagcggt caaaacaggc cgcagtaagg cggtcgggat agttttcttg
    39121 cggccctaat ccgagccagt ttacccgctc tgcgacctgc gccagctggc aggtcaggcc
    39181 aatccgtgcc ggatgcggtg tatcgctcgc tacttcaaca tcaacggtaa tcgccatttg
    39241 accactacca tcaatccggt aggttttccg gctgataaat aaggtttttc cctgatgctg
    39301 ccacgcgtga gcggtcgtaa tcagcaccgc gtcggcaagt gtatctgccg agcactgcaa
    39361 caacgctgct tcggcctggt aatggcccgc cgccttccag cgttcgaccc aggcgttagg
    39421 gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc agcggtgcac gggtgaactg
    39481 atcgcgcagc ggcgtcagca gttgtttttc atcgccaatc cacatctgtg aaagaaggcc
    39541 tgactgacgg ttaaattgcc aacgcttatt acccagctcg atgcaaaaat ccatttcgct
    39601 ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagt gtcacgctga ggttttccgc
    39661 cagacgccac tgctgccagg cgctgatgtg tccggcttct gaccatgcgg tcgcgttcgg
    39721 ttgcactacg tgtaccgtaa gccagagttg tccggcgctc tccggctgcg gtagtccagg
    39781 cagttcaatc aactgtttac cttgtggagc gacatccaga ggcacttcac cgcttgccag
    39841 cggcttgcca tccagcgcca ccatccagtg caggagctcg ttatcgctat gacggaacag
    39901 gtattcgctg gtcacttcga tggtttgccc ggataaacgg aactggaaaa actgctgctg
    39961 gtattttgct tccgtcagcg ccggatgcgg cgtgcggtcg gcaaagacca gaccgttcat
    40021 gcagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg ccgtaagcca accacgggtt
    40081 gccgttttca tcatatttaa tcagcgactg atccacccag tcccagacga agccgccctg
    40141 taaacgggga tactgacgaa acgcctgcca gtatttagcg aaaccgccaa gactgttacc
    40201 cattgcgtgg gcatattcgc aaaggatcag tgggcgcagt tctccaggca gcgaaagcca
    40261 ttttttgatg gaccatttcg gcaccgccgg gaagggttgg tcttcatcca cgcgcgcgta
    40321 catcgggcaa ataatatcgg ttgcgaaggt gtcggctccg ccgccttcat actgcaccgg
    40381 gcgggaagga tcgacagatt tgatccagcg atacagcgcg tcgtgattag cgccgtggcc
    40441 tgactcattc cccagcgacc agatgatcac actcgggtga ttacgatcgc gctgcaccat
    40501 tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga tcatcggtca gacgattcat
    40561 tggcaccatg ccgtgggttt caatattggc ttcatccacc acatacaggc cgtagcggtc
    40621 gcacagcgtg taccacagcg gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt
    40681 gttctgcttc atcagcagga tatcctgcac catcgtctgc tcatccatga cctgaccatg
    40741 cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag
    40801 cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat
    40861 cagcgtgccg tcggcggtgt gcagttcaac caccgcacga tagagattcg ggatttcggc
    40921 gctccacagt ttcgggtttt cgacgttcag acgtagtgtg acgcgatcgg cataactacc
    40981 acgctcatcg ataatttcac cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc
    41041 ctgccataaa gaaactgtta cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc
    41101 agcctccagt acagcgcggc tgaaatcatc attaaagcga gtggcaacat ggaaatcgct
    41161 gatttgtgta gtcggtttat gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca
    41221 catatcctga tcttccagat aactgccgtc actccagcgc agcaccatca ccgcgaggcg
    41281 gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca gacggcaaac gactgtcctg
    41341 accgtaaccg acccagcgac cgttgcacca cagatgaaac gccgagttaa cgccatcaaa
    41401 aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg tgagcgagta
    41461 acaacccgtc ggattctccg tgggaacaaa cggcggattg accgtaatgg gataggtcac
    41521 gttggtgtag atgggcgcat cgtaaccgtg catctgccag tttgagggga cgacgacagt
    41581 atcggcctca ggaagatcgc actccagcca gctttccggc accgcttctg gtgccggaaa
    41641 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    41701 ggcttcttcg ctattacgcc agctggcgaa agggggatgt gccgcaaggc gattaagttg
    41761 tgtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatccgtaat
    41821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaatttca cacaacatac
    41881 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    41941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    42001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
    42061 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
    42121 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
    42181 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
    42241 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
    42301 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
    42361 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
    42421 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
    42481 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
    42541 tcttcatggg agaaaataat actgttgatg ggagtctggt cagagacatc aagaaataac
    42601 gccggaacat tagcgcaggc tgcttccaca gcagtggcat cctggtcatc cagcggatag
    42661 ttaatgagca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
    42721 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcagcgcga
    42781 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
    42841 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
    42901 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
    42961 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtatagc
    43021 gttactggtt tcacattcac caccctgaat tgactctctt ccgaacgcta tcatgccata
    43081 ccgcgaaagg ttttgcgcca ttcgatggtg tcaacgtaaa tgcacgccgc ttcgccgtcc
    43141 ggccaccaga atagcctgcg attcaacccc ttcttcgatc tgttttgcta cccgttgtag
    43201 cgccggaaga tgcttttccg ctgcctgttc aatggtcatt gcgctcgcca tatacaccag
    43261 attcagacag ccaatcaccc gttgttcact gcgcagcggt acggcgatag aggcgatctt
    43321 ctcctcctga tcccagccgc ggtagttctg tccgtaaccc tctttgcgcg cgcgcgccag
    43381 aatggcttcc agctttaacg gttcccgtgc cagttgatag tcatcaccgg ggcgggaggc
    43441 taacatttcg attaattcct tgcggtcttg ttccgggcaa aaggccagcc aggtcaggcc
    43501 cgaggcggtt ttcagaagcg gcaaacgtcg cccgaccatt gcccggtgaa aggataagcg
    43561 gctgaaacgg tgagtggttt cgcgtaccac cattgcatca acatccagcg tggacacatc
    43621 tgtcggccat accacttcgc gcaacagatc gcccagcagt ggggccgcca gtgcagaaat
    43681 ccactgttcg tcacgaaatc cttcgcttaa ttgccgcact ttgatggtca gtcgaaaact
    43741 atcatcggag gggctacggc ggacatatcc ctcttcctgc agcgtctcca gcagtcgccg
    43801 cacagtggtg cgatgcaggc cgctgagttc cgccagcagc ccgacgctgg caccgccatc
    43861 aagtttattt aacatattta ataacattag accgcgggtt aagccgcgca cggttttgta
    43921 ttccgtctgc tcattgttct gcatattaat tgatatttct atagttaaaa caacgttgtg
    43981 cacctggtgc acattcgggc atgttttgat tgtagccgaa aacacccttc ctatactgag
    44041 cgcacaataa aaaatcattt acatgttttt aacaaaataa gttgcgccgt actgtgcgcg
    44101 caacggcatt ttgtccgagt cgtgaggtac tgaaatggca atacaacacc ctgacatcca
    44161 gcctgctgtt aaccatagcg ttcaggtggc gatcgcaggt gccggtccgg tcgggctgat
    44221 gatggcgaac tatctcggtc agatgggcat tgacgtgctg gtggtggaga aactcgataa
    44281 gttgatcgac tacccgcgtg cgattggtat tgatgacgag gcgctgcgca ccatgcagtc
    44341 ggttggcctg gtcgagaatg ttctgccgca cactacgccg tggcacgcga tgcgttttct
    44401 caccccgaaa ggccgctgtt ttgctgatat tcagccaatg accgatgaat ttggctggcc
    44461 gcgccgtaac gcctttattc agccgcaggt cgatgcggtg atgctggaag gggtgtcgcg
    44521 ttttcctaat gtgcgctgct tgttttcccg cgagctggag gccttcagcc agcaaaatga
    44581 cgaagtgacc ttgcacctga aaacggcaga agggcagcgg gaaatagtca aagcccagtg
    44641 gctggtagcc tgtgacggtg gagcaagttt tgtccgtcgc actctgaatg tgccgtttga
    44701 aggtaaaact gcgccaaatc agtggattgt ggtagatatc gccaacgatc cgttaagtac
    44761 gccgcatatc tatttgtgtt gcgatccggt gcgcccgtat gtttctgccg cgctgcctca
    44821 tgcggtacgt cgctttgaat ttatggtgat gccgggagaa accgaagagc agctgcgtga
    44881 gccgcaaaat atgcgcaagc tgttaagcaa agtgctgcct aatccggaca atgttgaatt
    44941 gattcgccag cgtgtctaca cccataacgc gcgactggcg caacgtttcc gtattgatcg
    45001 cgtactgctg gcgggcgatg ccgcgcacat catgccggtg tggcaggggc agggctataa
    45061 cagtggtatg cgcgacgcct ttaacctcgc ctggaaactg gcgttggtta tccaggggaa
    45121 agcccgcgat gtgctgctcg atacctatca acaagaacgt cgcgatcacg ccaaagcgat
    45181 gattgacctg tccgtgacgg cgggcaacgt gctggctccg ccgaaacgct ggcagggtac
    45241 gttacgtgac ggcgtttcct ggctactgaa ttatctgccg ccagtaaaac gctacttcct
    45301 cgaaatgcgc ttcaagccga tgccgcaata ttacggcggt gcgctggtac gtgagggcga
    45361 agcgaagcac tctccggtcg gcaagatgtt tattcagccg aaagtcacgc tggaaaacgg
    45421 cgacgtgacg ctgctcgata acgcgattgg cgcgaacttc gcggtaattg gctggggatg
    45481 caatccactg tgggggatga gcgacgagca aatccagcag tggcgcgcgt tgggcacacg
    45541 cttcattcag gtggtgccgg aagtgcaaat tcataccgca caggataacc acgacggcgt
    45601 gctacgcgtg ggcgatacgc aaggtcgtct gcgtagctgg ttcgcgcaac acaatgcttc
    45661 gctggtggtg atgcgcccgg atcgctttgt taccgccacc gccattccgc aaaccctggg
    45721 caatacgctg aataaactgg cgtcggtgat gacgctgacc cgccctgatg ccgacgtttc
    45781 tgtcgaaaag gtagcctgat atgcacgctt atcttcactg tctttcccac tcgccgttgg
    45841 tggggtatgt cgacccggcg caagaggtac tcgatgaggt caatggcgtg attgccagcg
    45901 cccgcgagcg tattgcggct ttctcccctg aactggtggt gctgtttgcg ccagatcact
    45961 acaacggctt tttctatgac gtgatgccac cgttctgttt aggcgttgga gcgacggcaa
    46021 ttggtgattt cggcagtgcg gcaggagagc tgcccgtgcc tgtggagctg gcggaggcct
    46081 gtgcgcatgc tgtcatgaag agcgggatcg atcttgccgt ttcttactgt atgcaggtgg
    46141 accacgggtt cgcccagccg ctggagttcc tgctcggtgg gctggataag gtgccagttc
    46201 tgcctgtgtt catcaacggt gtcgccacgc cgctgcccgg tttccagcgt acccgcatgt
    46261 tgggtgaagc cattggacgt ttcaccagca ctctcaataa acgcgtgctg ttcctgggtt
    46321 ccggtgggct ttcccatcag ccgccggtgc ccgaactggc gaaagccgat gcccatatgc
    46381 gcgaccgtct gttggggagc gggaaagatt tacccgccag tgagcgcgaa ttgcgtcagc
    46441 aacgggtgat tagcgccgct gagaagtttg ttgaggatca gagaactctg catccgctca
    46501 acccgatttg ggataaccag ttcatgactt tgctggagca gggacgcata caggaactgg
    46561 atgccgtcag taacgaagag ctttccgcca ttgccggaaa gtcgacacat gaaatcaaaa
    46621 cctgggtcgc cgcttttgcc gctatttcta catttggcaa ctggcgtagc gaagggcgtt
    46681 attaccgccc aatcccggag tggattgccg gatttggctc gttaagcgcc agaacagaga
    46741 actgaatatg caggagaaga tgatgagtta tcagccacaa accgaagccg ccaccagccg
    46801 ttttctgaat gtagaagaag cgggtaaaac gctgcgcatc cattttaatg actgcggaca
    46861 aggcgacgaa accgttgtcc tgctgcatgg ttccggcccg ggtgctactg gctgggcgaa
    46921 cttcagccgc aatatcgatc cgctggtaga ggcgggctat cgggtaatcc tgctggattg
    46981 tccgggttgg ggcaaaagcg attcgatcgt taatagtggt tcacgatcgg atcttaatgc
    47041 acgaatcctg aaaagcgtgg tggatcaact ggatatcgcc aaaatccacc tgctgggcaa
    47101 ctcgatgggc ggccatagtt ctgtggcgtt cacccttaac tggccggagc gcgtcggcaa
    47161 actggtgctg atgggcggcg gtacgggcgg catgagtttg tttacgccga tgccaaccga
    47221 aggtattaag cgactgaatc agctttatcg tcagccgact atcgaaaacc tgaagctgat
    47281 gatggatatc ttcgttttcg ataccagcga tttgaccgac gccctgtttg aagcgcgcct
    47341 gaataatatg ctgtcgcgcc gcgatcacca ggaaaacttc gttaagagcc tggaagctaa
    47401 tccgaaacag ttcccggatt ttggcccacg tctggcggaa atcaaagcgc aaaccctgat
    47461 tgtctggggg cgcaacgacc gctttgtgcc gatggatgcg ggtctgcgtc tgctgtccgg
    47521 cattgccggt tctgaactgc atatcttccg cgactgtggt cactgggcgc agtgggaaca
    47581 tgccgacgct ttcaatcaat tggtgctgaa tttcctcgca cgcccttaag gaatggtcat
    47641 gacgaagcat actcttgagc aactggcggc ggatttacgc cgcgccgcag agcagggcga
    47701 agcgattgcg ccgctgcgcg atctgattgg tatcgataac gctgaagcgg cctacgccat
    47761 tcagcacata aatgtgcaat atgacgttgt gcaggggcgt cgcgtggtag ggcgtaaagt
    47821 gggcctgacg catccgaaag tgcaacaaca actgggcgtt gatcaaccgg attttgggac
    47881 gttatttgcc gacatgtgtt atggcgataa cgaaatcatt cctttttccc gtgttctgca
    47941 accccgcatt gaagcggaga tcgcactggt gttgaaccgc gatttacccg caactgatat
    48001 caccttcgac gaactgtata acgccattga atgggtactt ccggcgctgg aagtggtggg
    48061 gagccgcatt cgcgactggt cgattcagtt tgtcgatacc gtggcagata acgcttcctg
    48121 cggggtgtat gtcatcggcg gtccggcgca acgtccggcg gggttagacc tgaaaaactg
    48181 cgccatgaag atgacgcgta ataacgaaga ggtttccagc gggcgcggca gcgaatgcct
    48241 gggacatccg cttaatgccg ccgtctggct ggcacgcaaa atggccagtc tgggtgaacc
    48301 gctgcgcgcc ggagatatca ttcttaccgg ggcattaggc ccgatggtgg cggtgaatgc
    48361 gggcgatcgt tttgaagccc atattgaagg cataggttca gttgctgcga cattttcaag
    48421 cgcagcccca aaaggaagtc tgtcatgagt aagcgtaaag tcgccattat cggttctggc
    48481 aacattggca ccgatctgat gattaaaatt ttgcgtcacg gtcagcatct ggagatggcg
    48541 gtgatggtcg gcattgatcc tcagtccgac ggtctggcga gtgccagacg tatgggcgtc
    48601 gccaccaccc atgaaggggt gatcgggctg atgaacatgc ctgaatttgc tgatatcgac
    48661 attgtatttg atgcgaccag cgcgggcgcg catgtgaaaa acgatgccgc tttacgcgaa
    48721 gcgaaaccgg atattcgctt aattgacctg acgcctgctg ccatcggccc ttactgcgtg
    48781 ccggtggtta acctcgaggc gaacgtcgat caactgaacg tcaacatggt cacctgcggc
    48841 ggccaggcca ccattccaat ggtggcggca gtttcacgcg tggcgcgtgt tcattacgcc
    48901 gaaattatcg cttctatcgc cagtaaatct gccggacctg gcacgcgtgc caatatcgat
    48961 gaatttacgg aaaccacttc ccgagccatt gaagtggtgg gcggcgcggc aaaagggaag
    49021 gcgattattg tgcttaaccc agcagagcca ccgttgatga tgcgtgacac ggtgtatgta
    49081 ttgagcgacg aagcttcaca agatgatatc gaagcctcaa tcaatgaaat ggctgaggcg
    49141 gtgcaggctt acgtaccggg ttatcgcctg aaacagcgcg tgcagtttga agttatcccg
    49201 caggataaac cggtcaattt accgggcgtg gggcaattct ccggactgaa aacagcggtc
    49261 tggctggaag tcgaaggcgc agcgcattat ctgcctgcct atgcgggcaa cctcgacatt
    49321 atgacttcca gtgcgctggc gacagcggaa aaaatggccc agtcactggc gcgcaaggca
    49381 ggagaagcgg catgaacggt aaaaaacttt atatctcgga cgtcacgttg cgtgacggta
    49441 tgcacgccat tcgtcatcag tattcgctgg aaaacgttcg ccagattgcc aaagcactgg
    49501 acgatgcccg cgtggattcg attgaagtgg cccatggcga cggtttgcag ggttccagct
    49561 ttaactatgg tttcggcgca catagcgacc ttgaatggat tgaagcagcg gcggatgtgg
    49621 tgaagcacgc caaaatcgcg acgttgttgc tgccaggaat cggcactatt cacgatctga
    49681 aaaatgcctg gcaggctggc gcgcgggtgg ttcgtgtggc aacgcactgt accgaagctg
    49741 atgtttccgc ccagcatatt cagtatgccc gcgagctcgg aatggacacc gttggttttc
    49801 tgatgatgag ccatatgacc acgccggaga atctcgcaaa gcaggcaaag ctgatggaag
    49861 gctacggtgc gacctgtatt tatgtggtgg attctggcgg tgcgatgaac atgagcgata
    49921 tccgtgaccg tttccgcgcc ctgaaagcag agctgaaacc agaaacgcaa actggcatgc
    49981 acgctcacca taacctgagt cttggcgtgg cgaactctat cgcggcggtg gaagagggct
    50041 gcgaccgaat cgacgccagc ctcgcgggaa tgggcgcggg cgcaggtaac gcgccgctgg
    50101 aagtatttat tgccgccgcg gataaactgg gctggcagca tgggaccgat ctctatgcgt
    50161 taatggatgc cgctgacgac ctggtgcgtc cgttgcagga tcgaccggta cgagtcgatc
    50221 gcgaaacgct ggcgctggga tacgctggtg tttactcgag cttcctgcgt cactgtgaaa
    50281 cggcggcggc gcgttatggc ttaagtgcgg tggatattct cgttgagctg ggcaaacgcc
    50341 ggatggttgg cggccaggag gatatgatcg ttgacgtggc gctggatctg cgcaacaaca
    50401 aataatgatg actgccgaga acgtgcattt tgtatgccca tcccagcacg actgccggat
    50461 gcggcgtaaa caccttatcc ggcctacggg tggcgtgaga atttgtaggc ctgataagac
    50521 gcgttagcgt cgcatcaggc atctgcacac gactgccgga tgcgataaac gtcttgtccg
    50581 gcctacattt cgcccgtagg cagtcattaa atagttctga ttacgcggct ggcaatgtat
    50641 cagtcgcgat tcacctgcac tcgcaacgag gttcatctta tgtcgactcg taccccttca
    50701 tcatcttcat cccgcctgat gctgactatc gggctttgtt ttttggtcgc tctgatggaa
    50761 gggctggatc ttcaggcggc tggcattgcg gcgggtggca tcgcccaggc tttcgcactc
    50821 gataaaatgc aaatgggctg gatattcagc gccggaatac tcggtttgct acccggcgcg
    50881 ttggttggcg gaatgctggc ggaccgttat ggtcgcaagc gcattttgat tggctcagtt
    50941 gcgctgtttg gtttgttctc gctggcaacg gcgattgcct gggatttccc ctcactggtc
    51001 tttgcgcggc tgatgaccgg tgtcgggctg ggggcggcgt tgccgaatct tatcgccctg
    51061 acgtctgaag ccgcgggtcc acgttttcgt gggacagcag tgagcctgat gtattgcggt
    51121 gttcccattg gcgcggcgct ggcggcgaca ctgggtttcg cgggggcaaa cttagcatgg
    51181 caaacggtgt tttgggtagg tggtgtggtg ccgttgattc tggtgccgct attaatgcgc
    51241 tggctgccgg agtcggcggt tttcgctggc gataaacagg ctgcgccacc actgcgtgcc
    51301 ttatttgcgc cagaaacggc aaccgcgacg ctgctgctgt ggttgtgtta tttcttcact
    51361 ctgctggtgg tctacatgtt gatcaactgg ctaccgctgc ttttggtgga gcaaggattc
    51421 cagccatcgc aggcggcagg ggtgatgttt gccctgcaaa tgggtgcggc aagcgggacg
    51481 ttaatgttgg gcgcattgat ggataagctg cgtccagtga ccatgtcgct actgatttat
    51541 agcggcatgt tagcttcgct gctggcactt ggaacggtgt cgtcatttaa cggtatgttg
    51601 ctggcgggat ttgtcgcggg gttgtttgcg acaggtgggc aaagcgtttt gtatgccctg
    51661 gcaccgttgt tttacagttc gcagatccgc gcaacaggtg tgggaacagc cgtggcggta
    51721 gggcgtctgg gggctatgag cggtccgtta ctggccggga aaatgctggc attaggcact
    51781 ggcacggtcg gcgtaatggc cgcttctgca ccgggtattc ttgttgctgg gttggcggtg
    51841 tttattttga tgagtcggag atcaagaatg cagccgtgtg ccgatgcctg acgcgcctta
    51901 ttaagcaggg gcgaaaggga gttgccccca atacacccac ggcgtttaca ggtatactcg
    51961 ctaaaaatta ttcagcgggt ttggaaacaa agatggcaaa acttacctta caagagcagt
    52021 tgctcaaagc aggattagtc accagcaaaa aagcggcgaa ggtggagaga acggcgaaaa
    52081 aatcgcgcgt tcaggcgcgt gaagctcggg cggcggtaga agaaaataaa aaggcacagc
    52141 ttgagcgtga taaacagctt agcgagcaac aaaaacaagc tgcgttggcg aaagaatata
    52201 aagctcaggt gaagcagctc attgaaatga accgaatcac cattgccaat ggcgatattg
    52261 gttttaactt cactgacggc aatctgatta agaagatttt tgtcgataag ctcacacagg
    52321 cgcagttgat taatggtcgt ctggcgattg ctcgcttgtt ggtcgataac aatagcgaag
    52381 gtgaatacgc cattattccc gccagcgttg ccgataaaat agctcagcgt gatgccagca
    52441 gtatagtgtt gcacagcgcg ctcagcgccg aagagcagga tgaagacgac ccatatgccg
    52501 acttcaaagt gcctgatgat ttgatgtggt aaacatagat aagtcgtgcg cagatgcctg
    52561 atgcgacgct atgcgcgtct tatcatgcct acggcctgcg ggcgaaatgt aggcggataa
    52621 ggcgttcacg ccgcatccgg cagtcgtgca ctattatcaa cgcatattca gtttattggc
    52681 gtggtaggca atatgctcgc caataaaact ggagacaaaa taatagctgt ggtcgtaacc
    52741 ctcgtgataa cggattaacg tcttgatatt catctcctgg cagatctttt caagatttgg
    52801 agtccgcagc tgttccgcgt aaaaatcatc actcaacccc tgatcaacca tgatttccgc
    52861 aacgcgttga ccttgtgaaa taagactcac cggatcgtaa ttcaaccagg catctttatt
    52921 ttcaccaaga tatgcggcaa aggcttgctg tccccacggt acttgcgatg gggagacaat
    52981 gggcgaaaac gccgagacgc tgacatattc atctgggtta cgtaacgcca gcaccagcgc
    53041 gcccagcccg cccatagaat gaccagagat agactttctg gtcgttgccg gaaaatgttg
    53101 catcactaaa tccggcagct cgttgcggat atagtcatac attttgtaat gttcattcca
    53161 cggcgcttgc gttgcgttca ggtaaaaccc ggcaccttgc ccgagatcgt aacggtcggc
    53221 atctgcgaca tgactgcctc gcggactggt gtccggcgca acaacaataa tgttgtgttc
    53281 agccgcgtaa cgctgcatcc ccgatttagt aatgaaattc tgctcgttgc aggtcaggcc
    53341 cgaaagccag tacagcaccg gcaatttttc attcgcggct tttggtggga gatagacgcc
    53401 gacattcatt tcacatttca gtgattggga ataatgccga tacacatttt gccagccgcc
    53461 aaagctggca tgtttttcaa tgagttccat cagtcattat ctcacggtta atcccccgga
    53521 cggctgccca gggagagtat taagaacccc gtctgaagtg gacggggtat acctgcggga
    53581 aatcagtaac gaattacggt tcgaatggat ttgccttcat gcatcaggtc gaaggcgtca
    53641 ttaatttcat caaggctcat ggtatgcgtg acaaacggtt ccagatcgat atcacctttc
    53701 atcgcatctt caaccatacc cggtaactgg gaacgacctt tcacgccgcc aaacgcggaa
    53761 cctttccata cgcgaccagt gaccaactgg aatggacggg tggagatttc ctgaccagaa
    53821 cctgccaccc cgatgatcac cgactgaccc cagccgcggt gcgcactttc cagcgccgca
    53881 cgcatcacgt tgacgttacc gatgcattca aaggtatggt cgatacccca tttgttgata
    53941 tccagcagga catctttaat cggtttgtcg tagtcattcg ggttaatgca gtcggtagca
    54001 ccgaagcgac gcgccagatc gaatttcttc gggttggtat cgatagcgat aatccgtccc
    54061 gctttcgcct gacgcgcgcc ctgaaccact gccagaccaa tcgcgccaag accaaacacg
    54121 gcaacagaat cacctggctg gactttagct gtgttgtgca ccgcgccaat accggtggtc
    54181 acgccacagc ccagcagaca gacgtgttca tggtttgctt ctggattaat tttggccaga
    54241 gacacttccg cgaccacggt gtattcactg aatgtagagc accccatgta gtgataaagc
    54301 ggctgcccgt tgtaagaaaa acgggtggtg ccgtctggca tcaagccttt accctgggtt
    54361 tcgcgaaccg caacacagag gttagttttg ccagaacgac agaactcaca ctcgccgcac
    54421 tccgcggtgt aaagcgggat cacatggtcg ccaggtttga cgctggttac gccttcaccg
    54481 acttcaacca caacgccggc cccttcgtga ccgagaacca ccgggaatac accttccggg
    54541 tcatccccgg agagggtaaa tgcgtcggta tggcaaacgc cggtatgggt gactttaatc
    54601 agcacttcac cttttttcgg tggtgcaacg tcaatttcaa cgatttccag cggtttaccg
    54661 ggagcaaatg caacggcagc acgtgatttc atctctcgct cttcctcaat atggtaatag
    54721 attcagctat ttaagatagg ctcgaaccag ttcaatagtg tcgtcaacgg attggctgac
    54781 ttcgcggctg tagcagtcat ttcggtcaaa cgtttcccgg atatggcttt caagcacttc
    54841 tgccatcagc ccgttagccg cgccccgaac ggcagcgatc tgttggagta tggcacggca
    54901 ttcggcatca ccctccatcg accgttccag agcatcaatc tgccctcgaa tacgacgaac
    54961 tcgagtaagg acctttttct tctcttccgg agtactgggc atttcgcacc tcatcatctg
    55021 cacgcaatat actatagggg ggtatactac atgtcaatgc atacccccct atagtatata
    55081 acactggaga ataaaattta tccggtgaat gtggtcggaa aacaaagagg aaaggggggg
    55141 gctaatcggc agggaaggcc gccccggata gcgggcggca gaaggaatca gaatttccag
    55201 gtcagatggg ctgcaagttg cagaccgtta taatcatcgg ttggggtgtc gtaccacact
    55261 ttacctgccg tcagcccgag attaagttgc tcagtcagcg gttgaatacg ttgcagactg
    55321 acgctcttca tgctgccgta gcgtgtttct ggcgtccagt cataggtgta agcgcctgtt
    55381 ccgcggctta gccatagttg cgtataacca gtgccgcgcg ggtcattcag gcgcacggaa
    55441 atcatattgc tataactacc acctgcatcg ctggagtcat aatgggtata gcggtagctg
    55501 gtgatcaccg ggccagtata gagtgatacg ccgccttgcc aggcatcgac ttcgacatca
    55561 tcgtaatatt tagtgtagcg ataaccggtc gtgaaaagcg tttttggcaa cagggccagg
    55621 ttaatatcct gacgaaaatt ctggcgggca aagaccggcg tattatccgc aaaggcaata
    55681 cccgttcggg ttgtcagcca gttattccat ttataccaga ccgcaccctg accgcgagtg
    55741 gcattgaaat gttcagtttc ataatcacga cgcccctggg aaagattaaa aagcagcgtt
    55801 gcgttttcaa ctttcgccac cagttcagca tacgctaaat tacggttgcc atgatcgcca
    55861 gaataatcgg taaaatcgta gcccgcggta atggaagtta acccggcata agcgggcaat
    55921 gcgctaaaaa tggccgccgc taataacgtc cgttttatca ttttaatctc ctgtacggat
    55981 aagttcttgt cggagtgaat aagttccggc aggtaaatta cgttctacaa tcacgcgcgg
    56041 gccaagttgc gtgttcgggg aaataattcg cccaggcaaa attataactt gtacgccaag
    56101 gcgtgaacgc tgcccgatat aacaacctaa tttatcgcat ccggtagcga taattccctc
    56161 tggagttcga acagacacag gctgttcatc cagacgatga ttactggtac gtacttgtgc
    56221 gcccaaatat gcctggtttg caactaccga gtcggcaata aaacattgcg gaccaatcgt
    56281 tgcttccgct tcaataaccg catttttaat ttcggtggca aaaccaattt ttacaccatt
    56341 gctgattatt gtgccaggac gaataaacgc ataattacct atcaggcagt ttgcgccaat
    56401 cactaccgga ccctgaataa cggcaccatg acaaatacgg gtattcgcgc cgataacaac
    56461 ttcacctgca gtttcatcaa taattacctg gtctgcaatc tgaatatttt tctctttaac
    56521 attaagcttc agatagtttt tcagttcctg tgacagaagg ttgggaataa gcggttgacg
    56581 cttccaccag tgcggaatag cgggtgggtt tgtcgcgctg aggttcgcgt ccagtaaaaa
    56641 aggcaataag tccataaata atccagatgg cataagctaa taatacatac acaacggaaa
    56701 gcggcgctaa aggcaccaac aaccagcaac gatgaaacca ggcgctaaaa gcaccaataa
    56761 cacaaactac gccgatccag ataagcggaa acattgccaa caccacacca tgcggcccgg
    56821 tggtgatgaa ttcattaaac caggtagtga gataaatccc aacgccataa agcacaacca
    56881 acagcatagg aaatatactg aagataccga agcggctaaa taaaagtctt ttatgcaggc
    56941 gcatacagac cgcgtatccc acaatccaac gccgccagcg acgccactcc tcacgcgggc
    57001 tgttgcattc ctgtgggtat acgatgcagc gattcgcctg ccgaatacgg tagccgtttg
    57061 ccactaatgt ccaggtgaga tcaaggtctt cgactttagt acgatccgag aaaccgaact
    57121 tacgcaatac atcagtacgg aacattccgc aggcaccgct gataataaac ggtgcgccgc
    57181 ccaggagctg ctgtagcgtg cgcttcataa caatcatcgg caactttacg gtcgcgcgga
    57241 tgtgcggtaa cagccccgcg cctttcaacg cagtagaggg aatgccgcct acggcatcag
    57301 caccgcgctc aatttctgcc agcatatagc ccattccgtc ttgatcgggc ggaacatagg
    57361 tgtcggcatc acttaaaaaa acctggtcgc aggtggcata attgaggcca ttcatcagcg
    57421 caccaccttt accggtattt ttttgcgtga cggcaacaaa gcggtcgccc catttgcgtt
    57481 tgacttccgc catgaccgct tcggtattgt ccgtggagcc gtcgttaacg caaattaccc
    57541 ggcaaaaata agggttacgc agtagattat ccagtgactg cgccagacac gggccttcgt
    57601 tatacgcagg tataatggcg tcaatacagc ctttcttttg actgggttta cgtcttagcg
    57661 tacttaaaaa ccatagcaac attgctatag acatacagat aaatatccag gttttcatgc
    57721 aatttgctca aagaatcatt ttatgaatta caaagccttc acccagatcg ctattgacct
    57781 gttgcccgcg aaattgtgca actgcacgca ggcgatcatg acgcatataa tcacgtcgtt
    57841 cctggctttt atgttttttt aatgcagcaa gtttgactgt gaaatattct tctttaacgg
    57901 attcaaaaac ctgaggcata aatgaaagcc aggtacttgg ggtttcgtag ccgagaattt
    57961 gtggaatagt gcggcaggca accattgaag cttgataaac agcgagatga tcctgatggc
    58021 ggtcggcatc atgcatggta tatacccgca tgatttcaac atcagaagga atttgatttt
    58081 taatgatgtc ttccagagcg gaaatcatat cattgagctg taaatgagcg cgggtgtcag
    58141 caaaattaag atgaatagtt tggtggcacc ctaatatctt tagggcgttg cgcgattctt
    58201 catggcgatc gattattcca tctgtaccag agttgccggt agtcatcacc acggctgcga
    58261 tataaattcc tttttgcgca agacgagcaa gcgatgcgcc acagcctaat tctatatcgt
    58321 caggatgtgc gccaatagca aggataccct ttcttttatt tgccgaagaa gggagggctg
    58381 aatctaaaac cttatccact taatgacact ccattttatt tattatacta caagcacaac
    58441 gatgcactca gagacgtaat ctctggcgca gccagatgtt aatttataaa tgttaaatgt
    58501 tgctaataac tgaaaagcaa gggctttcag acccggaggt tcgactgggt aatgttttta
    58561 tgcaattcat atgttaagtg tttgtatgtt ttgtatgtat agttattttg ttttatacat
    58621 tgtatttaga tgtgatttag tttgtcaatt aattgcattt aaaaaatatg ttctgtgaac
    58681 aagtattgtt tatatacatt atgtgaatgt aatatgcgag tgattgagaa tgatacagtg
    58741 atatatacaa tgcgaatata atagttttat tatatgtatt gatattgata gaaataatga
    58801 agtaataaat ctcgtaatgt ggttgtttat gcatcactaa aatgaagtgt agtaattttc
    58861 ccaattgtta gaacggagta attgcatatt taatctttcc tttgccgttt ttttgctaag
    58921 aataaaatca tctgtgcgat aacgactaat tcttttaatg aatgttttta ttcctgaata
    58981 ctgctcacat aacaagacag gggagcagac aatcatggca atttcatcgc gtaacacact
    59041 tcttgccgca ctggcattca tcgcttttca ggcacaggcg gtgaacgtca ccgttgcgta
    59101 tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac aacacctttg ctaaagaaag
    59161 cggagcaacc gtggactggc gtaagtttga cagcggagcc agcatcgtgc gggcgctggc
    59221 ttctggcgat gtgcaaatcg gcaacctcgg ttccagcccg ttagcggttg cagccagcca
    59281 acaggtgccg attgaagttt tcttgctggc gtcaaaactg ggtaactccg aagcgctggt
    59341 ggtaaagaaa actatcagca aaccggaaga tctgattggc aaacgcatcg ccgtaccgtt
    59401 tatctctacc acccactaca gcctgctggc ggcgctgaaa cactggggca ttaaacccgg
    59461 gcaagtggag attgtgaacc tgcagccgcc cgcgattatc gctgcctggc agcggggaga
    59521 tattgatggt gcttatgtct gggcaccggc ggttaacgcc ctggaaaaag acggcaaggt
    59581 gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca acgctggacg tctgggtggt
    59641 gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa gcgttcgcta aaagcgccat
    59701 cgatgctcag caaccgtaca ttgctaaccc agacgcgtgg ctgaaacagc cggaaaacat
    59761 cagcaaactg gcgcgtttaa gcggcgtgcc ggaaggtgac gttccggggc tggtgaaggg
    59821 gaatacctat ctgacgccgc agcaacaaac ggcagaactg accggaccgg tgaacaaagc
    59881 gatcatcgac accgcgcagt ttttgaaaga gcagggcaag gtcccggctg tagcgaatga
    59941 ttacagccag tacgttacct cgcgcttcgt gcaataaaag gaggcgcaga tgctgcaaat
    60001 ctctcatctt tacgccgatt atggcggcaa accggcactg gaagatatca acctgacgct
    60061 ggaaagcggc gagctactgg tggtgctggg gccgtccggc tgcggtaaaa ccaccctgct
    60121 gaatctgatt gccggttttg tgccttatca gcatggcagc attcaactgg cgggtaagcg
    60181 tattgaggga ccgggagcag agcgcggggt ggtttttcag aatgaagggc tgctaccgtg
    60241 gcgcaatgta caggacaacg tggcgttcgg cctgcaattg gcaggtatag agaaaatgca
    60301 gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg ctggaaggcg cagaaaaacg
    60361 ctatatctgg cagctttccg gtggtcaacg tcagcgggtg gggattgccc gcgcgctggc
    60421 ggcgaatccc cagctgttat tactcgacga gccgtttggt gcgctggacg ccttcacccg
    60481 cgaccagatg caaaccctgc tgctgaaact ctggcaggag acgggcaagc aggtgctgtt
    60541 gattacccac gatatagaag aagcggtgtt tatggcgact gaactggttc tgctttcacc
    60601 cggccctggc cgcgtgctgg agcggctgcc gctcaacttt gctcgccgct ttgttgcggg
    60661 agagtcgagc cgcagcatca agtccgatcc acaattcatc gccatgcgcg aatatgtttt
    60721 aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg tgctcattaa tgaaaaactg
    60781 cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc aggtgacctt aagcattggc
    60841 acgttagcgg ttttactcac tgtatggtgg acggtggcgg cgctgcaact gattagcccg
    60901 ctgtttttgc cgccgccgca acaagtactg gcaaaactgc tcaccattgc cggaccgcag
    60961 ggatttatgg acgccacgct gtggcagcat ctggcagcca gcctgacgcg cattgtgctg
    61021 gcgctactcg cggcggtggt gatcggtatt ccggtcggga tcgccatggg gctaagcccg
    61081 acggtgcgcg gcattctgga tccggtaatc gaactttatc gtccggtgcc gccgctggct
    61141 tatttgccgc tgatggtgat ctggtttggt attggtgaaa cctcgaagat cttactgatc
    61201 tatttagcga tttttgcacc ggtggcgatg tcggcgctgg cgggggtgaa aagcgcgcag
    61261 caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc gtgcgcaggt gctgtggttt
    61321 gtcattttgc ccggcgcgct gccggaaatc ctcaccggat tacgcattgg tctgggtgtg
    61381 ggctggtcta cgctggtagc ggcggagctg attgccgcga cgcgcggttt aggatttatg
    61441 gttcagtcag cgggtgaatt tctcgcgact gacgtggtgc tggcggggat cgcggtgatt
    61501 gcgattatcg cctttctttt agaactgggt ttgcgcgcgt tacagcgccg tctgacgccc
    61561 tggcatggag aagtacaatg agtgaacgtc tgagcattac cccgctgggg ccgtatatcg
    61621 gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag cgataatcag tttgaacagc
    61681 tttaccatgc ggtgctgcgc catcaggtgg tgtttctgcg cgatcaaact attgcgccgc
    61741 agcagcaacg cgcgctggcc cagcgttttg gcgaactgca tattcaccct gtttaccctc
    61801 atgccgaagg ggttgacgag atcatcgtgc tggataccca taacgataat ccgccggata
    61861 acgacaactg gcataccgat gtgacattta ttgaaacgcc acccgcaggg gcgattctgg
    61921 cagctaaaga gttaccttcg accggcggtg atacgctctg gaccagcggt attgcggcct
    61981 atgaggcgct ctctgttccc ttccgccagc tgctgagcgg gctgcgtgcg gagcatgatt
    62041 tccgtaaatc gttcccggaa tacaaatacc gcaaaaccga ggaggaacat caacgctggc
    62101 gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt ggtgcgaacg catccggtga
    62161 gcggtaaaca ggcgctgttt gtgaatgaag gctttactac gcgaattgtc gatgtcagcg
    62221 agaaagagag cgaagccttg ttaggctttt tattcgccca tatcactaaa ccggagtttc
    62281 aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg ggataaccgc gtgactcagc
    62341 actatgccaa tgccgattac ctgccacagc ggcggattat gcatcgggcg acgatccttg
    62401 gggataaacc gttttatcgg gcggggtgat acgagtgcgg acggttccct cgcccccttg
    62461 gggagagggt tagggtgagg gggcgttcac cgtactttca acaggttaac tccccctttc
    62521 tgagaggaaa aaaattaacg cagaatcttc ttctcagcca aatccagcgc aaagtagctg
    62581 aaaatcagat ccgcacccgc acgcttaatc gaacctaagc tttcgagcac gactttctct
    62641 tcatctatag cacccgccag cgcggcgaac ttaatcatcg cgtactcacc gctcacctga
    62701 tacgcgccaa tcggcaattc agtacgttca cgcagctcac gcacgatgtc gaggtatgct
    62761 ccagcaggtt taaccatcag gcagtctgcg ccctgggctt catccagcag tgattcacga
    62821 atcgcctcgc gacggttcat tgggttcatc tgatagcttt tgcggtcgcc ttttaatgcg
    62881 cttccggcag cttcacggaa cggaccataa aaggaagagg cgaacttggt cgaatacgac
    62941 ataatcgccg tatctttaaa gcctgcagcg tccagcgcct ggcgaatagc ctgtacctgg
    63001 ccatccatcg cggcggaagg ggcgatgaag tctgcgcctg cagcagctgc aaccacggct
    63061 tgcttgccta aattttccag agttgcgtcg ttgtcgacgc catgctcgca cagcacaccg
    63121 cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg acatgacgat catttctggc
    63181 acagtctgct tgcagatgcg cgacatacgc gccaccagtc catcttcccg ccaggcgtcg
    63241 ctgccggttt catcggtatg gtgagagatg ccaaaagtca ttacggaacg gataccggcg
    63301 ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg gaatgcgcat cacgcctggc
    63361 atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa caaagatcgg caacaccagg
    63421 tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca gcgcaggaga tttgcgcagg
    63481 cgacgagggc gttggattaa gtctgtcatg gtctgcctga tgtttgtgga attaaggtgc
    63541 acagtatacc tgaagcgggg caggagtgtt ttacgaaagt tgtcgtgatg ttgacaagaa
    63601 gagaatgaaa gagagaccag gacat
//
LOCUS       DAZFDP010000009        58690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFDP010000009 DAZFDP010000000
VERSION     DAZFDP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..446
                     /gene="yfdR"
                     /locus_tag="ACHJ1G_000661"
     CDS             <1..446
                     /gene="yfdR"
                     /locus_tag="ACHJ1G_000661"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4762566.1"
                     /translation="SNICRFAGHLSHFYSVAQHAVLCSQLVPQEFAFEALMHDATEAY
                     CQDIPAPLKRLLPDYKRMEEKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDLGLD
                     DGSFWPVLEGIPATEMFKVIPLSPGHAYGMFMERFNELSGLRKCA"
     gene            437..583
                     /locus_tag="ACHJ1G_000662"
                     /pseudo
     CDS             437..583
                     /locus_tag="ACHJ1G_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            560..1024
                     /locus_tag="ACHJ1G_000663"
                     /pseudo
     CDS             560..1024
                     /locus_tag="ACHJ1G_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1026..1217
                     /locus_tag="ACHJ1G_000664"
     CDS             1026..1217
                     /locus_tag="ACHJ1G_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762567.1"
                     /translation="MNGQISIVRPGACDDSEIRMIIRLARGKTITVLITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            1220..1810
                     /locus_tag="ACHJ1G_000665"
     CDS             1220..1810
                     /locus_tag="ACHJ1G_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4762568.1"
                     /translation="MTTFTNSKLTDEYVSNATLIRLILWADQHNSHYVVAALRELQER
                     RKADSAEPVSQTYKLPVNTPCQDAPAHIWLQTAGVWPEDGELSELTWCSHNQHHDDTL
                     YVRADLVNGNYPATPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGD
                     WFNDGKPTWDDGDGEDLYLKEVTHWMQLPEPPQEVK"
     gene            1898..2140
                     /locus_tag="ACHJ1G_000666"
     CDS             1898..2140
                     /locus_tag="ACHJ1G_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4762569.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2144..2278
                     /locus_tag="ACHJ1G_000667"
     CDS             2144..2278
                     /locus_tag="ACHJ1G_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762570.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVAKRMFDVWCHCRG
                     "
     gene            2297..2551
                     /gene="xisR"
                     /locus_tag="ACHJ1G_000668"
     CDS             2297..2551
                     /gene="xisR"
                     /locus_tag="ACHJ1G_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4762571.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2585..3871
                     /locus_tag="ACHJ1G_000669"
     CDS             2585..3871
                     /locus_tag="ACHJ1G_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4762572.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTDKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            3892..4593
                     /gene="mlrA"
                     /locus_tag="ACHJ1G_000670"
     CDS             3892..4593
                     /gene="mlrA"
                     /locus_tag="ACHJ1G_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4762573.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            4653..4760
                     /gene="yohO"
                     /locus_tag="ACHJ1G_000671"
     CDS             4653..4760
                     /gene="yohO"
                     /locus_tag="ACHJ1G_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4762574.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4741..5472)
                     /gene="yehW"
                     /locus_tag="ACHJ1G_000672"
     CDS             complement(4741..5472)
                     /gene="yehW"
                     /locus_tag="ACHJ1G_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4762575.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5477..6403)
                     /gene="yehX"
                     /locus_tag="ACHJ1G_000673"
     CDS             complement(5477..6403)
                     /gene="yehX"
                     /locus_tag="ACHJ1G_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4762576.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6396..7553)
                     /gene="yehY"
                     /locus_tag="ACHJ1G_000674"
     CDS             complement(6396..7553)
                     /gene="yehY"
                     /locus_tag="ACHJ1G_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4762577.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7560..8477)
                     /gene="osmF"
                     /locus_tag="ACHJ1G_000675"
     CDS             complement(7560..8477)
                     /gene="osmF"
                     /locus_tag="ACHJ1G_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4762578.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8688..10985)
                     /gene="bglX"
                     /locus_tag="ACHJ1G_000676"
     CDS             complement(8688..10985)
                     /gene="bglX"
                     /locus_tag="ACHJ1G_000676"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4762579.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11181..12896
                     /gene="dld"
                     /locus_tag="ACHJ1G_000677"
     CDS             11181..12896
                     /gene="dld"
                     /locus_tag="ACHJ1G_000677"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4762580.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12934..13866)
                     /gene="pbpG"
                     /locus_tag="ACHJ1G_000678"
     CDS             complement(12934..13866)
                     /gene="pbpG"
                     /locus_tag="ACHJ1G_000678"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4762581.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14040..14627)
                     /gene="yohC"
                     /locus_tag="ACHJ1G_000679"
     CDS             complement(14040..14627)
                     /gene="yohC"
                     /locus_tag="ACHJ1G_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4762582.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14797..15375
                     /gene="yohD"
                     /locus_tag="ACHJ1G_000680"
     CDS             14797..15375
                     /gene="yohD"
                     /locus_tag="ACHJ1G_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4762583.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15505..16266)
                     /gene="yohF"
                     /locus_tag="ACHJ1G_000681"
     CDS             complement(15505..16266)
                     /gene="yohF"
                     /locus_tag="ACHJ1G_000681"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4762584.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16319..17755)
                     /gene="mdtQ"
                     /locus_tag="ACHJ1G_000682"
     CDS             complement(16319..17755)
                     /gene="mdtQ"
                     /locus_tag="ACHJ1G_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4762585.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17979..18062
                     /gene="yohP"
                     /locus_tag="ACHJ1G_000683"
     CDS             17979..18062
                     /gene="yohP"
                     /locus_tag="ACHJ1G_000683"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4762586.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18450..19400)
                     /gene="dusC"
                     /locus_tag="ACHJ1G_000684"
     CDS             complement(18450..19400)
                     /gene="dusC"
                     /locus_tag="ACHJ1G_000684"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4762587.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19639..20037
                     /gene="yohJ"
                     /locus_tag="ACHJ1G_000685"
     CDS             19639..20037
                     /gene="yohJ"
                     /locus_tag="ACHJ1G_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4762588.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            20034..20729
                     /gene="yohK"
                     /locus_tag="ACHJ1G_000686"
     CDS             20034..20729
                     /gene="yohK"
                     /locus_tag="ACHJ1G_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4762589.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20859..21743
                     /gene="cdd"
                     /locus_tag="ACHJ1G_000687"
     CDS             20859..21743
                     /gene="cdd"
                     /locus_tag="ACHJ1G_000687"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4762590.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21893..22612
                     /gene="sanA"
                     /locus_tag="ACHJ1G_000688"
     CDS             21893..22612
                     /gene="sanA"
                     /locus_tag="ACHJ1G_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4762591.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22615..22854
                     /gene="yeiS"
                     /locus_tag="ACHJ1G_000689"
     CDS             22615..22854
                     /gene="yeiS"
                     /locus_tag="ACHJ1G_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4762592.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            23173..24411
                     /gene="preT"
                     /locus_tag="ACHJ1G_000690"
     CDS             23173..24411
                     /gene="preT"
                     /locus_tag="ACHJ1G_000690"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4762593.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            24405..25640
                     /gene="preA"
                     /locus_tag="ACHJ1G_000691"
     CDS             24405..25640
                     /gene="preA"
                     /locus_tag="ACHJ1G_000691"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4762594.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25711..26721)
                     /gene="mglC"
                     /locus_tag="ACHJ1G_000692"
     CDS             complement(25711..26721)
                     /gene="mglC"
                     /locus_tag="ACHJ1G_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4762595.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26737..28257)
                     /gene="mglA"
                     /locus_tag="ACHJ1G_000693"
     CDS             complement(26737..28257)
                     /gene="mglA"
                     /locus_tag="ACHJ1G_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4762596.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(28318..29316)
                     /gene="mglB"
                     /locus_tag="ACHJ1G_000694"
     CDS             complement(28318..29316)
                     /gene="mglB"
                     /locus_tag="ACHJ1G_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4762597.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29596..30636)
                     /gene="galS"
                     /locus_tag="ACHJ1G_000695"
     CDS             complement(29596..30636)
                     /gene="galS"
                     /locus_tag="ACHJ1G_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4762598.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(30778..31935)
                     /gene="yeiB"
                     /locus_tag="ACHJ1G_000696"
     CDS             complement(30778..31935)
                     /gene="yeiB"
                     /locus_tag="ACHJ1G_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4762599.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLCYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31952..32620)
                     /gene="folE"
                     /locus_tag="ACHJ1G_000697"
     CDS             complement(31952..32620)
                     /gene="folE"
                     /locus_tag="ACHJ1G_000697"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4762600.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32878..33714
                     /gene="yeiG"
                     /locus_tag="ACHJ1G_000698"
     CDS             32878..33714
                     /gene="yeiG"
                     /locus_tag="ACHJ1G_000698"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4762601.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33746..35737)
                     /gene="cirA"
                     /locus_tag="ACHJ1G_000699"
     CDS             complement(33746..35737)
                     /gene="cirA"
                     /locus_tag="ACHJ1G_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4762602.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSLITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(36030..37499)
                     /gene="lysP"
                     /locus_tag="ACHJ1G_000700"
     CDS             complement(36030..37499)
                     /gene="lysP"
                     /locus_tag="ACHJ1G_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4762603.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37704..38585)
                     /gene="yieE"
                     /locus_tag="ACHJ1G_000701"
     CDS             complement(37704..38585)
                     /gene="yieE"
                     /locus_tag="ACHJ1G_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4762604.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            38684..39733
                     /gene="yeiH"
                     /locus_tag="ACHJ1G_000702"
     CDS             38684..39733
                     /gene="yeiH"
                     /locus_tag="ACHJ1G_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4762605.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            39807..40664
                     /gene="nfo"
                     /locus_tag="ACHJ1G_000703"
     CDS             39807..40664
                     /gene="nfo"
                     /locus_tag="ACHJ1G_000703"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4762606.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            40668..41756
                     /locus_tag="ACHJ1G_000704"
     CDS             40668..41756
                     /locus_tag="ACHJ1G_000704"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4762607.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(41812..43062)
                     /gene="nupX"
                     /locus_tag="ACHJ1G_000705"
     CDS             complement(41812..43062)
                     /gene="nupX"
                     /locus_tag="ACHJ1G_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4762608.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43162..44103)
                     /gene="rihB"
                     /locus_tag="ACHJ1G_000706"
     CDS             complement(43162..44103)
                     /gene="rihB"
                     /locus_tag="ACHJ1G_000706"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4762609.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            44233..44931
                     /gene="yeiL"
                     /locus_tag="ACHJ1G_000707"
     CDS             44233..44931
                     /gene="yeiL"
                     /locus_tag="ACHJ1G_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4762610.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(44999..46249)
                     /locus_tag="ACHJ1G_000708"
     CDS             complement(44999..46249)
                     /locus_tag="ACHJ1G_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4762611.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46343..47281)
                     /gene="psuG"
                     /locus_tag="ACHJ1G_000709"
     CDS             complement(46343..47281)
                     /gene="psuG"
                     /locus_tag="ACHJ1G_000709"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4762612.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(47269..48210)
                     /gene="psuK"
                     /locus_tag="ACHJ1G_000710"
     CDS             complement(47269..48210)
                     /gene="psuK"
                     /locus_tag="ACHJ1G_000710"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4762613.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(48634..50325)
                     /gene="fruA"
                     /locus_tag="ACHJ1G_000711"
     CDS             complement(48634..50325)
                     /gene="fruA"
                     /locus_tag="ACHJ1G_000711"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4762614.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(50342..51280)
                     /gene="fruK"
                     /locus_tag="ACHJ1G_000712"
     CDS             complement(50342..51280)
                     /gene="fruK"
                     /locus_tag="ACHJ1G_000712"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4762615.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(51280..52410)
                     /gene="fruB"
                     /locus_tag="ACHJ1G_000713"
     CDS             complement(51280..52410)
                     /gene="fruB"
                     /locus_tag="ACHJ1G_000713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4762616.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            52778..53959
                     /gene="setB"
                     /locus_tag="ACHJ1G_000714"
     CDS             52778..53959
                     /gene="setB"
                     /locus_tag="ACHJ1G_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4762617.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(53956..54210)
                     /gene="yeiW"
                     /locus_tag="ACHJ1G_000715"
     CDS             complement(53956..54210)
                     /gene="yeiW"
                     /locus_tag="ACHJ1G_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4762618.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            54365..54937
                     /gene="yeiP"
                     /locus_tag="ACHJ1G_000716"
     CDS             54365..54937
                     /gene="yeiP"
                     /locus_tag="ACHJ1G_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4762619.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            55160..56626
                     /gene="yeiQ"
                     /locus_tag="ACHJ1G_000717"
     CDS             55160..56626
                     /gene="yeiQ"
                     /locus_tag="ACHJ1G_000717"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4762620.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            56744..57730
                     /gene="yeiR"
                     /locus_tag="ACHJ1G_000718"
     CDS             56744..57730
                     /gene="yeiR"
                     /locus_tag="ACHJ1G_000718"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4762621.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            57769..58482
                     /gene="lpxT"
                     /locus_tag="ACHJ1G_000719"
     CDS             57769..58482
                     /gene="lpxT"
                     /locus_tag="ACHJ1G_000719"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4762622.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
BASE COUNT        14540 a        15410 c        14507 g        14233 t
ORIGIN      
        1 tttcaaatat ctgtcgcttt gcaggacatc tttcacactt ctacagcgtc gcccaacatg
       61 cggtgctttg cagccagctg gtaccgcagg aatttgcttt tgaagcgtta atgcatgatg
      121 caacagaagc atattgccag gacatccccg caccactgaa acgccttctt cctgactata
      181 aacggatgga agaaaaaata gacgccgtaa tccgtgagaa atacgggtta cctcctgtta
      241 tgagcacgcc agtgaaatat gccgatctca ttatgctggc aaccgaacgc cgcgatctcg
      301 ggcttgatga tggttctttc tggcctgtac tggaaggtat cccggcgaca gagatgttca
      361 aagttattcc actgtcacca ggccatgcct acgggatgtt tatggaacgt tttaacgagt
      421 tatcggggtt acgcaaatgc gcatgaatgt tttcgaaatg gaagggtttc ttcgcgggaa
      481 atgtgtaccg cgagatctga aagtgaacga aacaaacgct gagtacctag tgcgtaaatt
      541 tgctgaagct gaggccaagt gcgcggcgct ggctgaacgt atcgaagagt tacagacaaa
      601 acctacgcca gattcgtttg gcatcatcgg tgaaaatatt cgaacacagg ataatcgaat
      661 aacgtcagac cctatgtttt gtgtgtatca aaagcgcgaa attgttgttg atgctgatta
      721 tgactatgac cggattgtct gggttgatga agatggcaat gaagccaata aactccaaag
      781 tcgtcgtctc gaactacttc atgaaaactt tcgggaacca ccagaaaaat ggcggcgcgt
      841 tgctgtgaaa gatattgatg aattcgttac ctgctgtttc accgaacagg gttgtaaaga
      901 ctacctggca gccaatggtc acaatcttcg cttgccattt atatatgtaa aaagcggttt
      961 caggaacgct gaatatatcg gcataagaac ctggcttgct ggcattcgca tcaaaggaga
     1021 gtgagatgaa cggacaaata tcaattgttc gaccaggagc atgtgacgat agcgagatac
     1081 gcatgattat tcgtctggcg agggggaaaa caataactgt tctcattact ccagaaaatc
     1141 tcgcattagc attaacagga aagtcagacc tgccagtaga gctaaagctg cgaaatgttg
     1201 agattaaggt gaaatagcta tgaccacttt taccaatagc aaactaacag acgaatacgt
     1261 ttcaaatgca acgttgattc ggctcattct gtgggctgac cagcacaata gccattatgt
     1321 tgtagcggct ctgcgcgagc tacaggagcg ccgcaaggct gatagtgcag aacctgtaag
     1381 ccaaacttac aagttgccag ttaatacacc ttgccaagat gcgccagccc atatctggct
     1441 gcaaacggct ggagtatggc cagaagatgg cgagttaagc gaattaacgt ggtgcagcca
     1501 caatcaacac catgatgaca cgctatatgt tcgagctgac cttgtgaatg gcaactatcc
     1561 ggcaactccg gatggttgga taagctgtag tgagcgaatg cctgaaatgg gagagcgaca
     1621 atgctatgtg ttagcagctg actttaaaaa caactaccca ccaaacatcc ccaacactca
     1681 ggtcggcgta tatggcgact ggtttaatga tggcaagccc acttgggatg acggtgatgg
     1741 cgaagacctg tatctcaaag aggtaaccca ctggatgcag ctaccagaac cgccgcagga
     1801 ggtgaaatga tgaattggcc tgaagcattc accgcagttg gagttgcaat agcggtggca
     1861 tttattctgt attcgctttt ccgctgggga taaaggaatg ttcgctctga ttcaacgtgg
     1921 tcagatatac acggacagag ccggataccc tgtggtgatt actcgcagta ctcagcactc
     1981 agtgttcttt cgacgcatgg acgggcgctc cggacgggta cgcattggtg agttcaacaa
     2041 cctgttcgaa catattgacc aacaggagta ccgcaaaatt ctggcgggca ctgagcagga
     2101 aatgcgcctg aaaaaattac gcgcaatgca acggaggtga tacatgcata cggcttttga
     2161 gttctgggtt cgcaagacat tcggcaatcg ctacgacctg acccgtgatg tcgacggctt
     2221 ctactgccgt gaagttgcga aacgaatgtt tgacgtgtgg tgccactgcc gtggatgaaa
     2281 gttttatgag gttggcatgc agacaatcat ctatcagata acccccagca aatggtgtac
     2341 ggagagagtc cttattgcat caacagggct aaagcccggc accatcgagc gggccagaag
     2401 aaagtcatgg atgcagggaa aagaataccg ccattacgct gtagaaggtg atcctgggca
     2461 ttacagtgaa tgcctgtaca acatcgaaga aattatgcga tggatcgaaa accagaaaca
     2521 accaggtgcc aaaaatgcaa gttccggtta acctgttaat gctcctggac gtctgggagg
     2581 tttaatgagt aacgcatcat acccgacagg cgttgaaaac catggaggat cactccgtat
     2641 atggtttcac tataatggca aacgtgtcag agaaaacctc ggtgttcctg acacagacaa
     2701 aaaccggaag atcgctggtg aacttcgcac ttccgtttgt tttgcaatca gaatggggag
     2761 tttcgactac gccgcgcagt tccctaattc ccctaacctg aaacactttg gtctgggaaa
     2821 aagagagata accgttaagg cactttcgga aaaatggttg gaccttaaga aaattgagat
     2881 ttgtgcgaat gcacttaacc gttaccagtc agtaattaaa aacatgttac caatgttagg
     2941 tgaaaaaaaa ctggtttcat ccataacaaa agaggattta cttttcgtaa ggagagattt
     3001 gttgaccggt taccaaaagc tttctaatgg aaagacttct tccataaaag ggcgctcagt
     3061 ggtcacggta aactactata tgacaaccat agctggaatg tttcaatttg caacagataa
     3121 tggttatacc tcaggaaacc catttaacgg tctggctccc ttaaaaaagt ccaaggtaaa
     3181 accagatcct ctcacccgtg acgaatttat tcgttttatt gaggcttgcc gtcatcaaca
     3241 aacaaaaaac ctgtggattc tcgctgtata cacgggtatt cgtcacgggg agttggtatc
     3301 gctggcatgg gaagatatag accttaaagc aaggactata accatccgta gaaattatac
     3361 aaaacttggc gaattcactc caccaaaaac cgatgcaggc accggaagga caattcatct
     3421 ggttcaacca gctattgatg ctcttaaaag ccaggcggaa atgaccatgc ttggaaagca
     3481 acattctgta gaggtgaagc agagggaata tgggagaact gctgtgcata aatgcacttt
     3541 tgtttttagt cctcaggtaa caaaacagca gcagttgtcc ggacctcact acaaggttga
     3601 ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac acagaaaatc
     3661 gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gcaggagcta atcctagttt
     3721 tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt acggagcatg
     3781 gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat ctgaaagtgt
     3841 cccatgtatg ccccataaga aagcagggta aaataaaaac ttgcaaaatc aattagttta
     3901 cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct gaaaccgcaa
     3961 cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccgtat ccgcgagatc
     4021 aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa
     4081 aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ctacctgcaa
     4141 agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc
     4201 cagacactca ccacacatct gtttattcct ctgcgccgac gacttcaatg ccaacaaccg
     4261 actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat cgccatttgt
     4321 ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg gaatattcag
     4381 gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat
     4441 gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggtcg tacattgctg
     4501 gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa
     4561 caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg
     4621 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
     4681 ccctgttcct gtttatggcg ttcggcggaa ttggtggcgt catgctcgca ggttatacct
     4741 ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat
     4801 cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag
     4861 cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt
     4921 accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg
     4981 tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg caacttctgt
     5041 cacgctggca tcgatcgctc ccagcccggc aagtgtcgcc tgcaggacgg gcagcacacc
     5101 gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg gaacggcaat
     5161 cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg
     5221 gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc caatgatcac
     5281 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact
     5341 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
     5401 gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca gcggatcgcg
     5461 caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc gtaccgcaag
     5521 gctggccctg cgtgttcacc accggcagca cctcgcaacc ccgcgcgaca aacagggaga
     5581 gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt tcttcgcgac
     5641 gcacgtaatc cgccacacta cgtaacgaaa gcagacgcac acccagttcg ctacgtccaa
     5701 aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttgccctgct
     5761 gcaccacttc accgtgatcc atcaatacca gatgttcagc cagccgtagc gcctcatcaa
     5821 tatcatgagt gaccagcaca atagtgcgcc ccagcaaacg gtgaatgcgc gtcatctctt
     5881 gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc atcagtaaga
     5941 cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga ccaccggaaa
     6001 gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgtc attaattcgt
     6061 cgatacgatc gtcaatccgt gcccgcgacc atttttgtaa ttgcggcacg gtagcgatgt
     6121 tttgcgccac gctccagtgg gggaataggc caatagattg aatggcatag cccatccggc
     6181 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atctctccgc
     6241 tgtcatgttc caccaggcgg ttaatcattt tcaaggtggt ggatttgccg gagccagatg
     6301 tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attaagatcg ttaacggctt
     6361 tttgtgcgcc aaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
     6421 agtgcgataa gcaaatcgaa cagggcatcg atcagcaccg ccagaacaat taccgggatc
     6481 acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca
     6541 aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg caccatcacc
     6601 acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg caggaatcgc
     6661 tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
     6721 aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
     6781 gcgggtgtca ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtcacc
     6841 agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg
     6901 acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca ccagatgccc
     6961 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
     7021 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag tgagagatcg
     7081 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
     7141 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc
     7201 agcgccgcag ccagccagaa accactgccg aggctggtac gcgccagcgc actgccattt
     7261 tgcgccagtt gtgtcgccgc ctttcccgcg ccccacacca gcaatacgaa gacgaattgc
     7321 gccagaatga gtgcacaaat gctgcctttt ttaccgggaa taaaacaggc cgtcagccag
     7381 gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca gagatgacgc
     7441 ccttcacccg agactaaacg attaggcgcg tagctgataa acggcagcgc tgctgcgatt
     7501 gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt cacgggaaat
     7561 tacttcgccc accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct
     7621 tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct ggcgaagact
     7681 ggctgtagcc actgtgccat ttgcggatac tccttcaaca ccgattcacg caccactggt
     7741 gcaggcgcat agataggttg cacgccttgc ggatcgctta aggtttgcag ccccagcgcc
     7801 gctaccgagc cgtcagtgcc gtaagccatt gcagcattaa cgccagaggt ttgctgggca
     7861 gcggctttga tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg
     7921 agcttaaagc cataggcttt ttcaaacgcg ggtaacgcat cggcgcgttc gataaactct
     7981 gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat aacgactcag gtcggcaagc
     8041 gaagtgagtt tatttttctc tgccacatcc tgacgcacgg cgatagtcca ggtgttattt
     8101 gcaggcgcgg gcgtcagcca gattaacttg ttgtgctccg aatcgagctt tttgactttc
     8161 tcgtaacctt gctgcgcgtt tttccacgct gcatcgtttt catctttaaa gaagaaagcg
     8221 ccattgccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc cccgcgcacc
     8281 acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct ttccagcacc
     8341 tgcaaaatga tattgccgag tagcgcacct tcggtatcga tttttgaacc gactttaacg
     8401 ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga acctgcccag
     8461 agctttaaga gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag
     8521 agaaaagcat agttgataat ggcggggtta gcttgagcgg aaggattcag ttgcagaatc
     8581 agataaatac ttaagaggca tattcggtcc ggcgtttcct gccggatgcg gcgcgagcgc
     8641 cttatccggc ctacaaaggg cgcaaacgtc gcgcccttac taaagcatta cagcaactca
     8701 aactcgcctt tcttaacgcg tgcggaatca gtgccgataa atacattgaa cttgccaggc
     8761 tcggcgtcat atttcatctg ttgattccag aacttcagcg cctcaatatc gatcgggaag
     8821 ctgacagtct gagtttcgcc cggtttcagg gtgattttct caaagccttt cagctgtttc
     8881 acagggcgac tcatggaagc cgtcacatcc tgcaagtaca tctgcactac cgtggccccc
     8941 tcgcgcttac cggtgttcgt cacctgcacg ctggcagtca ctttgccgtc acgcttcatg
     9001 gtcggcgcag aaagtttcac atcagagacg gtgaaagtgg tgtagctcag cccatagccg
     9061 aacggataca acgccccgtt agcttcatca aaataacgcg aagtgtattt gttcggcttg
     9121 tcggcattat acgggcgacc ggtattcaga tggctgtagt acaccgggat ctgcccgaca
     9181 gaacgcggga aggacattgg cagcttgccg gacgggttgt aatcgccaaa caatacatcg
     9241 gcaattgcat taccgccttc agtccccgca aaccaggttt ccagaatcgc atcagcctgc
     9301 tgatcttctt tcaccagcgc cagcggacgc ccgttcatca gcaccagcac cagcggttta
     9361 ccggtggctt tcagcgccgc aatcaagtca cgttggcttt gcggaatagt gatatcggtc
     9421 cggctggagg cttcgtgcgc catcccctgt gcttcaccga ctacagccac caccacatca
     9481 gattgtttcg ccgtctgcac cgcttcatca atcatctctt gcggcgaacg cggatcgact
     9541 ttgaccgctt cttcatactg attcaggaaa tcgataatgc ctttgtcact ggtaacgttc
     9601 gcccctttgg catacagcac tttaccgttt tcaccgacgg aatttttaat cccggtcagt
     9661 acggtcacgg attgatcggc aacaccggct gcggaccagc tgcccatcac gtcacgttta
     9721 ctgtccgcca gtggcccaac caccgcaatg gtggccgatt ttttcagcgg taacgtttcg
     9781 agacggtttt tcagcaacac caagctttcg cgcgccactt cacgcgcttc tttacggtgc
     9841 aggcggcttt cggcattggt atccaccggg tcagactctt tcggccccaa atggctgtat
     9901 gggtcgttaa acaatcccat atcatattta acgttcagta catggcgggc agcatcgtcc
     9961 agctcttcca tcgtcacttt gccggatttg atcaacccag gcagatactt cgagtaatac
    10021 tcgtcgctca tactcatgtt gattccggat ttcagcgcca cgcgcaccgc atcttccggg
    10081 tctgccgccg tgccatgttt aatcagctct ttgattgcac cgtgatcgga aacggtgatg
    10141 cctttaaagc cccactggtc gcgcagaaca tctttcagca gccaggaatc ggaggtggct
    10201 ggcgtgccgt tcagcgagtt cagcgccacc atcaccgcgc cgctgcctgc gtccagcccc
    10261 gctttgtacg gcggcatata atcattaaac aggcgctgcg gactcatatc gacggtgttg
    10321 tactctttac cgccttctac cgcgccgtat gcggcaaagt gtttgacgct ggtcatcacc
    10381 gagtagcgat ctgccgggct tttaccctgc atcgcttcca ccatggtttt acccattgtt
    10441 gaggtgagat acgtatcttc gccaaaacct tcggaagcac gtccccagcg cggatcgcgc
    10501 gagacatcga ccatcggtgc ccaggtcata ttcaggccat catctgccgc ttcataagca
    10561 gagacacgtc cgaccgtttt cactgcatcg aggttaaaag acgaggccag accgaggcta
    10621 atcgggaaca ccgtgcgctg accgtgcagc acgtcgtaag caaagaaaag aggaattttc
    10681 aggcggctta attccatcac ctgatcctgc atggcgcgga tatcctgacg ggttacggtg
    10741 ttgaaaatcg ccccaacctg accgtctttg atcatctcgc ggatcgcctc tttcgggtta
    10801 tccgggccga cgctgattaa gcgcagctga ccaattttct catcaactgt cattttctta
    10861 agcagttcgg tgacgaacgc atcccgcgct tcgggcgtta atggatggtt gccgaataaa
    10921 tcatccgcca gtgctggctg cagggccaga ctcaccgcga ttcctactga acatagccat
    10981 ttcatatgga tttttcttcc tctgttgccc gacgaggcag cgaaaatggt gcaaaaaccg
    11041 tagtttgcca taagcatgat ggagagagaa aaagaatgct cagtttattg tctgaatttt
    11101 caaaatattc actcgctgaa ttgtcataca aggcgctatt ctagtttgtg atattttttc
    11161 gccaccacaa ggagtggaaa atgtcttcca tgacaacaac tgataataaa gcctttttga
    11221 atgaacttgc ccgtctggtc ggtcattcac acctgctcac cgatcccgca aaaacggccc
    11281 gctatcgcaa gggcttccgt tctggtcagg gcgacgcgct ggctgtcgtt ttccctggct
    11341 cactactgga attgtggcgg gtgctgaaag cctgtgtcac cgccgacaaa attattctga
    11401 tgcaggctgc caatacaggc ctgaccgaag gatcgacgcc aaacggtaac gactatgatc
    11461 gcgatatcgt gatcatcagc accctgcgtc tcgacaagct gcacgttctt ggcaagggcg
    11521 aacaggtgct ggcctatccg ggcaccacgc tctattcgct ggaaaaagcc ctcaaaccgc
    11581 tgggacgcga accgcactcg gtgattggat cgtcgtgtat aggcgcatcg gttatcggcg
    11641 gtatttgtaa caactccggc ggctcgctgg tgcaacgtgg cccggcgtat accgaaatgt
    11701 cgttattcgc gcgtataaat gaagacggca aactgacgct ggtgaaccat ctggggattg
    11761 atctgggcga aacgccggag cagatcctta gcaagctgga tgatgatcgc atcaaagatg
    11821 acgatgtgcg tcacgatggt cgtcacgccc acgattatga ctatgtccac cgcgttcgtg
    11881 atattgaagc cgacacgccc gcacgttata acgccgatcc ggatcggtta tttgaatctt
    11941 ctggttgcgc cgggaagctg gccgtctttg cggtacgtct agataccttc gaagcggaaa
    12001 aaaatcagca ggtgttttat atcggcacca accagccgga agtgctgacc gaaatccgcc
    12061 gtcatattct ggctaacttc gaaaatctgc cggttgccgg ggaatatatg caccgggata
    12121 tctacgatat tgcggaaaaa tacggcaaag acaccttcct gatgattgat aagttaggca
    12181 ccgacaagat gccgttcttc tttaatctca agggacgcac cgatgcgatg ctggagaaag
    12241 tgaaattctt ccgtccgcat tttactgacc gtgcgatgca aaaattcggt cacctgttcc
    12301 ccagccattt accgccgcgc atgaaaaact ggcgcgataa atacgagcat catctgctgt
    12361 taaaaatggc gggcgatggc gtgggcgaag ccaaatcgtg gttggtggat tatttcaaac
    12421 aggccgaagg cgatttcttt gtctgtacgc cggaggaagg cagcaaagcg tttttacacc
    12481 gtttcgccgc tgcgggcgca gcaattcgtt atcaggcggt gcattccgat gaagtcgaag
    12541 acattctggc gttggatatc gctctgcggc gtaacgacac cgagtggtat gagcatttac
    12601 cgccggagat cgacagccag ctggtgcaca agctctatta cggccatttt atgtgctatg
    12661 tcttccatca ggattacata gtgaaaaaag gcgtggatgt gcatgcgtta aaagaacaga
    12721 tgctggaact gctacagcag cgcggcgcgc aataccctgc cgagcataac gtcggtcatt
    12781 tgtataaagc accggagacg ttgcagaagt tctatcgcga gaacgatccg accaacagca
    12841 tgaatccggg gatcggtaaa accagtaaac ggaaaaactg gcaggaagtg gagtaaaaat
    12901 tacggatggc agagtatcgc catccgaatt cacttaatcg ttctgtgccg tctgccccgc
    12961 cgccgccatt tgggcggctt tttgtttttt atagctcaac gctgctgccg gcacaggcat
    13021 cactttaccg gtttcaatcc aggtacgcag gcggctggca tcggcaaaat gggtatattt
    13081 gccaaacgcg tccatcacta ccagcgccac cggtttatta ttgataaccg tacgcatcac
    13141 cagacaatgg cccgccgcat tggtaaagcc ggttttggtt aactgaatat tccagttatc
    13201 gcgatacacc agatgattag tattgcggaa cggcagcgta tacgtcgggt tagagaaggt
    13261 tgccatatct tcccgggtag tacttaactg cccgatcaac ggatactgtt tgctggcaat
    13321 gagcagtttg gttaagtcac gggcagttga aacgttatgc accgacaatc cggtaggttc
    13381 aacaaagcgc gtgttgttca ttccgagcga tttcgctttc gcattcattg ccttaataaa
    13441 ggctttgtaa ccaccgggat aatggtgcgc aaggcttgcc gccgcgcggt tttctgaaga
    13501 catcagcgcc agcaacagca tatctttacg gctgatttcg ctattcagtc gtacgcgcga
    13561 atagacccct ttcatctccg gcgtctggct gatatccact tttagttttt catccagcgg
    13621 cagtcgtgca tccagcacaa ccatcgcggt cattaatttg ctgatagacg caatcggacg
    13681 caccagatcc gggtggttcg aatagatcac tttgttggta ttcagatcaa caatcatcgc
    13741 gctaccggag gcaatttccg gttgtgaagc ggtggtagcg gctgccgttt tcgcaacggc
    13801 ctgcggtgcc aaaggcacag ccagcatcag ggccaggcta aataaagaaa ctcgaaattt
    13861 cggcatgatg agcattcaga tagtggttca cgcgcacggg ttgcgcaccg ccggagtaag
    13921 gatttactga ggctagcgac gccatcataa cgagcaaaaa gtgcgatcgt caaaggagaa
    13981 tcgtgaggaa atgctgcatt gctgacattt acgccagcaa tgcaatgtca aagaactttc
    14041 tagaacaggc gatacccgta gccccatagt ataacggtta gggcgagcag cacttccagt
    14101 accagcacgc caatcgccag cgtcgaactg gagaagctaa ggccttcctc tttgttgata
    14161 ttcaggaagc tcggaatacc aaggtaaagc aggtagccgg tgtaaaacag cgccaccgtg
    14221 ccgaccagcg cacacaacca gaccagcgga taaagcgcca ccagaccgct taaaaacagc
    14281 ggagttgcaa cgtagcccgc gaagaccatg cagtgcgcaa gtgacggacg ctgcgggtaa
    14341 ttgcgcgcca tccaccagat gacccgcccc atcaccgcaa ccccagcgag cataacgcca
    14401 taaaacagga cagccagcgc cagtccggta aaccaggaca acttcaggat agtgccatcg
    14461 ccaaaattcc agccaatctg tgtagtgcca atgaaggcgc aaatcaccgg aatcgccgcc
    14521 atcagcaaaa cgtggtgggt gtaatggtga gaaatcgttt cgttttcgcg attaatcacc
    14581 tgcatttcac gatcgggatg ggaaaacagt ccccagacat ggctcatacc gcccccttgt
    14641 tgtgagttca tgaacctgac agttcaagta taagtcagct tgtgattatt ttttgttcgc
    14701 tcatgtaatt tgcaccgtct gatgtacgtc ctttttcagc gtgaatgatt tccagggtgt
    14761 atgcttacag gcaaccaaag ggagacagac tggcctatgg atctcaatac actcatctca
    14821 caatatggtt atgccgcgct ggtgatcggt agcctggcgg aaggtgaaac cgtgactttg
    14881 ctgggaggcg ttgcggcgca tcaggggcta ttaaagttcc cgctggtggt actttctgtg
    14941 gcgcttggcg gcatgattgg cgaccaggtg ctttatctgt gcgggcggcg gtttggcggc
    15001 aagctgttac gccgtttctc gaaacatcag gataaaattg agcgggcgca gaaacttatc
    15061 caacgccatc cgtatctgtt tgtcattggt acgcgcttta tgtatggctt tcgggtgatt
    15121 ggcccgacgc tgattggtgc cagccagctg ccgccgaaaa tctttctgcc gcttaatatt
    15181 ctcggcgcat ttgcctgggc gttgattttt accactatcg gttacgctgg tggtcaggtg
    15241 attgcgccgt ggttgcacaa tctcgatcag catttgaagc actgggtctg gttgattctg
    15301 gtcgtggttc tggtggtagg cgtgcgctgg tggctgaaac gacgcgggaa gaaaaagccg
    15361 gataatcagg cgtaaaacca ttgccctgga taaggcgttc gcgccgcatc cgacatccgg
    15421 tgcaccatgc ctgatgcggc gcttgtgcgt cttatcatgc ctaccaaaat tttacccatt
    15481 gaaatatcca cacacctcac tgcgctattc tgggttgaac tgcggattcg ccaacataaa
    15541 gccgccatcc actatcaacg actgcccggt ggtgtaattt gcgccctccg aacaaagcca
    15601 cgccaccagg ctggcaatct catacgttgc gccaaaacgc cgcagaggaa tcgaaggctc
    15661 cgcgtcgggc ttcacgtcgc tatcatccat gccgttcatt ggcgtagcga tcgccccagg
    15721 tgcgactgcg ttcaccaaaa tcttatgcct gaccaactcc agcgccattg ctttggttaa
    15781 cccgccgagc gcatgtttag cggctgtgta ggcgctggca tccggcagcg gcgtatgttc
    15841 atgtaccgac gtaatgttga tgatgcgacc accctgccct tgtttcacca tctggcgggc
    15901 cgcaatttgt gagcataaaa atgcaccatc gacatcaacg gtaaaaatct tgcgccactc
    15961 atcgaaagcc atatcaagaa acggcgcttt ggtcattgcg cccgcattat tcaccagcac
    16021 atcaatacgc cccagccgtt gaatgagttt ctccagcgcc tgtgcacctt ctggcagttt
    16081 accgagatcc agctgcacaa tctccgcacg tacgccgtgg ctaactacct cacgcgcggt
    16141 atcttttgcc ccttcttcat ctgagtgcca ggtaatgcca atatcaaacc cctgctgcgc
    16201 cagtaataac gcgcactctt tgccgatccc cgaatcggag gcggtaataa tcgcaacctg
    16261 tgccatcgag ttctccactt aacgctgaat aaacgttaag tatagaaggc gcatatcatc
    16321 agcgtttgta acccccgccc aacgcaccag tgagttgaat ggaagcatcc agccactgcc
    16381 cttgcaataa caggccattg gcccgctcac gcagcgcggg gattctggct tcgctgacgc
    16441 gggaaccagc aatgatgccc gcgttaaagc gcgcctgcgc aagacctacc acacgcaagg
    16501 catcgcgctc aatttgcgcc tgatgctggt ttttctccgc aagtgtctga acctgactgg
    16561 ctgcccgcgc cacgtcattc accgcttcaa ccaccgcttt gttgtagctg gcgatagaca
    16621 agttgctttc ggcttttgcg atatcgagat tggcgttaag acgaccacta tcgaaaatgg
    16681 gtagcgtcag gcctgccgta acgcccattt gctgcgcgga atgacggaac agatcgctta
    16741 agtgcaacgc atcctgttgc aggaaggcca tcaggttgat gtcaggataa aatgccgctt
    16801 ttgccgcatc aatggtgctt agcgatgact caacgtacca gtgcgccgcc tgcaaatctg
    16861 cccgccgggc cagtaaggag taccccagtt catcaggaag ctggcttgcc actttcggca
    16921 acgcgaccgg tttaagcttc aatgactttg tctggttatt tgtaagtgcg cttaaccgtg
    16981 cctcaataat tttcattttc cccgcgacat cgttgagctg ctgccgggtt ttgctggcat
    17041 taatatcggt ttccacacct tcaactgaag aagtaatccc gttctgatat agctggcgat
    17101 cggtcgcgat aatggtgttc tgctcttttt ctatttgctg caagaccgtg tttaacgccg
    17161 cctgggtttg ccactcccag tacaggcggg ctacgctgcc agccagcaat tggcgggttt
    17221 gctcgcgttc cgccgcccgt gctttaaccg tacccaggcg ggcagtaacc tccgcccgat
    17281 tctttcccca gatatcgaga tgccagcccg ccgttaagcc aaaagttccg ttggtgtacc
    17341 acggaccggt cgtacctgcg gccggatcgt tcagagcaaa cggccccatt aagccttctg
    17401 ccgacatttt ttgccgctcc atatccgcag aaaagtcgat ctgcggacca tcctgagtag
    17461 caattgcctt cgcctgggct tcagctagct gaatgcgctg ttcagccacc tgcatatccg
    17521 gtgcgttctg tagtgcattg ttaattaagg aagtgagttg attatcgtga tactccagcc
    17581 accattggct gtctggccaa ccatttttca gcgccgtggg taatgcggtg tcaacttgtg
    17641 cagcgggcgt ttgctggctt aacgcctggc gggtttcatg cataggcgca cacccggcca
    17701 gcatcagtaa cagcggaaaa caggcgatgg cgggataaaa ggaatcacga ttcatggggg
    17761 aataatcagg taagaaaagg tgcgcggaga ttaccgtgtg ttgcgatata ttttttagtt
    17821 tcgcgtggca atacatcagt agcaataaaa cgacatatcc agaaaaatat acactaagtg
    17881 aatgatatct tccgatttat cttaatcgtt tatggataac ggcaaagggc ttcgtttttt
    17941 cctatactta ttcagcactc acaaataaag gaacgccaat gaaaattata ctctgggctg
    18001 tattgattat tttcctgatt gggctactgg tggtgactgg cgtatttaag atgatatttt
    18061 aaaaatcaga tatggcatat aagttgtcag gtcaggaact agatattttg tgtacttgat
    18121 ttgcaaacag ctccggtgtt ggtggcgtca aaatcgctga agcgttcccc gaaggccggg
    18181 gcagcccaca tggatgtggg ctgagggcgc gtcttacaag gacgttacct cgcgcccgac
    18241 ccggtagacg aaggggataa gctgaaggta ccgcgcagcg gcgattttgt tcgccagagc
    18301 ccgggggtgc agggggagga ggcgattgtc ctccccctgc gcgctccttg cgccagtggc
    18361 aatatgtggc ttagctcatg aaaggagcgc aacaagatga tgaagctgaa taagcgattc
    18421 attgtctctg ataaactgcg attccactat tatcgcagct tatcgatatc aattgcctga
    18481 atagcccttg caatatcagg ggaattattc aacacccgaa catgctgaaa taattccgtt
    18541 gcttcatcgt attctttacg caaataactc aaccactgtt taatccgcgc aacgtgatat
    18601 aacccggtat cgccctgctt ttccagacgg gtatattttt gcagcaaagc aaccacctcc
    18661 ggccacggca ttcgcggttc gttatatttt actacccggc tcaggttggg aatattgagc
    18721 gccccgcgac caatcatcac tgcgtcgcag ccgctgatcg caatgcattg ttgcgcgctc
    18781 tgccagtccc agatttcgcc gttggcaatc accggaatat tcagccgctg gcgaatctcg
    18841 ccaatcgcct gccagtcaat atgctccgcg cggtaaccct gctcttttgt ccgtccatgc
    18901 accaccagct ccgtagcacc agcctgttga accgcatcgg cgatttcaaa tttcttctca
    18961 ccgctgtccc agcccagacg cactttcacg ctgacgggca aatgcgccgg tacagcttca
    19021 cgcatcgctt ttgcaccctg gtagatgagt tcaggatctt tgagtaacgt cgccccacca
    19081 ccgctaccgt tgaccgtttt cgacgggcag ccgcaattga gatccacgcc ccaggaacct
    19141 aattccaccg cacgggcggc gttctctgcc agccattgtg ggaactgacc caacaactgc
    19201 acgcgcacca gcgtaccgga tggtgtccgg ctggcgtttt gtagctcagg gcaaatgcga
    19261 tgaaaaactt ttaccggcag cagttgatcc accacgcgga caaactcggt gatgcacaga
    19321 tcgtagtcgt taacttcggt cagcaattca cgcaccagag agtcaagcac accctccatc
    19381 ggagccagta acacacgcat atcttcaccc gcaaaaaaat gaggcgctat gttagcgcct
    19441 atggtcaacg gattaaaggt ctgcaaagag ggattgctgc aaactgtaat ccctctctat
    19501 gcttgatgta tgcctgagtt cgttgcgcat tgcgtaatgc cggggagcga aaaaataagc
    19561 gctgatttct taatgtgatc ggtagcacgt tttacgaatt aattgtatga tgaatccatc
    19621 tcatctgggg tgttgattat gagtaagaca ctgaacatta tctggcaata tttacgcgct
    19681 ttcgtcctga tttatgcctg cctgtatgca ggcattttca ttgcttccct gctaccggta
    19741 accattccgg gcagcatcat cggtatgctg atcctgtttg tcctgctggc cttgcaaatt
    19801 cttccggcaa aatgggtcaa tccggggtgc tacgtactga ttcgctatat ggcgctattg
    19861 tttgtgccga ttggcgtagg cgtcatgcaa tattttgatt tgctccgcgc acagtttggc
    19921 ccggtagtgg tttcctgtgc aatcagtacg ctggtggttt ttctggtggt gagctggagt
    19981 tcgcaactgg tacacggtga acgtaaagtc gtaggtcaga aaggatcaga agaatgatgg
    20041 caaatatctg gtggtcatta ccgctgacgt tgattgtctt ttttgccgcc cgcaaactgg
    20101 cggcacggta taagtttccc ctgcttaacc cgttgctggt agcaatggtg gtgatcattc
    20161 cttttttaat gctgaccggc atctcttacg acagctactt taaaggtagc gaagtgctaa
    20221 acgatctgct gcaaccggcg gtcgtcgcgc tggcctatcc tttatatgag cagctacacc
    20281 agatccgcgc gcgctggaaa tcgatcatca ccatctgttt tatcggcagc gtggttgcga
    20341 tggtgacggg gacttccgtg gcattgttga tgggcgcttc accggaaatc gccgcgtcaa
    20401 tcctgccgaa atcagtcacc acgcctattg caatggcggt tggcggcagt attggcggta
    20461 ttccggcaat cagcgcagtt tgcgtgattt tcgtcggcat cctcggcgcg gtatttggcc
    20521 atacattgct taatgcgatg cgtattcgta ccaaagctgc gcgcggtctg gcaatgggga
    20581 ctgcctcgca cgccctcggt acggcgcgct gcgccgagct ggattatcag gaaggtgcat
    20641 ttagttcgct agcgctggtg ttatgcggga taattacttc gctgatcgca ccgttccttt
    20701 tcccgattat tctggcagta atgggctaaa atttgcgatg cgtcgcgcat ttttgatgta
    20761 agtttcacgc gttgcataat taatgagatt cagatcacat ataaagccat aacgggttcg
    20821 taaactgtta tcccattaca tgattatgag gcaacgccat gcatccacgt tttcaaaccg
    20881 cttttgccca acttgcggat aacttgcaat ctgcactgga acctattctg gcagacaagt
    20941 acttccccgc tttgttgacc ggggagcaag tctcatcgct gaagagcgca acggggctgg
    21001 acgaagacgc gctggcattc gcactacttc cgctggcggc ggcctgtgcg cgtacgccat
    21061 tgtcgaattt taatgttggc gcaattgcgc gcggtgtgag cggaacctgg tatttcggtg
    21121 ccaatatgga atttattggt gcgacaatgc agcaaaccgt tcatgccgaa caaagcgcga
    21181 tcagccacgc ctggttgagt ggtgaaaaag cgcttgcagc catcaccgtt aactacacgc
    21241 cttgtggtca ctgccgtcag tttatgaatg aactgaacag cggtctggat ctgcgtattc
    21301 atctgccggg ccgcgaggca cacgcgctgc gtgactatct gccggatgcc tttgggccga
    21361 aagatctgga gattaaaacg ctgctgatgg acgagcagga tcacggctat gcgctgactg
    21421 gtgatgcgct ttctcaggcg gcgattgcag cggcaaaccg ttcgcacatg ccttacagta
    21481 agtcgccaag cggtgtcgcg ctggaatgta aagacggtcg tattttcagt ggcagctacg
    21541 ctgaaaacgc cgcattcaac ccgactctgc caccgttgca gggagcgtta attctgttga
    21601 atctcaaggg ttatgattac ccggatatcc agcgcgcggt tctggcagaa aaagccgatg
    21661 cgccgttgat tcagtgggat gccacctccg caacgctgaa agctctcggc tgtcacagta
    21721 tcgaccgagt gcttctcgct taagcctggt gccggatgcg gcgtgaacgc cttgtccggc
    21781 ttgccagccc tctcctggtg tcgaaattcc cggcaaacag tttgccgttt cttgcgcaaa
    21841 accagcgggt aaagtagcct gatggaaatt ttccttagat cgagtctcct gcatgttaaa
    21901 gcgcgtgttc ctcagcctgt tagtcctgat cggcttgctg ctgttgactg tgctcggcct
    21961 cgatcgctgg atgagctgga aaaccgcgcc ttatatctac gacgaattgc aggatctccc
    22021 ctaccgccag gtcggtgtgg tgctcggaac agcaaaatat tatcgtactg gcgtaattaa
    22081 tcagtattat cgctaccgca ttcaaggagc gattaatgcc tataacagcg gtaaggtaaa
    22141 ttatctatta ctgagcggcg ataacgcatt gcaaagttat aatgagccga tgaccatgcg
    22201 caaagattta atcgctgctg gtgtcgaccc atcagatatt gttctcgatt acgcaggctt
    22261 tcgtacgctg gattccatcg tgcgtacacg caaagttttc gatactaatg atttcattat
    22321 tatcacccaa cgtttccact gtgagcgggc attatttatt gcgctgcata tggggattca
    22381 ggctcagtgt tatgccgtac cgtcaccgaa agatatgctg tcagtacgta ttcgcgaatt
    22441 tgccgcccgt ttcggtgcgc tggctgacct ttatattttt aaacgtgaac cgcgtttttt
    22501 agggccgctg gtccctattc cggctatgca ccaggtgccg gaagatgcgc aggggtatcc
    22561 cgccgtcaca cccgaacagt tacttgaatt acaaaagaaa caaggaaagt agttatggat
    22621 gtacagcagt tttttgtcgt tgccgttttt ttccttattc cgatattttg tttccgcgaa
    22681 gcatggaaag gctggcgcgc aggcgcgatt gataaacggg ttaaaaatgc accggaaccg
    22741 gtgtatgtct ggcgagcaaa aaatcccgga ctctttttcg cttatatggt ggcatatatc
    22801 ggcttcggaa ttttatctat cggcatgatt gtttatctta ttttctatcg ttaatatttt
    22861 ctcttttctg ttaattattg agaataatta ttacttcact caataagctg cggatataac
    22921 attctgaacc gcagctattt gtgaatcctt tcacagtata aatttcccca cgcgcatagc
    22981 cttaatatca gcacgcaata tttatactct aaataattcg agttgcagga aggcgacaag
    23041 tgagtgagtc cccaggagct tacataagta agtgactggg gtgagcgaac gcagacgcag
    23101 cacatgcaac ttgaagtatg acgagtatat ttagcgttcg ccttaattac tcatagcatt
    23161 aaggaagatc acatgccgca acaaaattat ctggatgaac tcactccggc ttttacgcct
    23221 ttactggcga ttaaagaagc ctctcgctgt ttattatgtc atgacgctcc ctgtagtcag
    23281 gcttgcccgg cacagaccga tccggggaaa tttattcgct caatctactt tcgtaatttt
    23341 aaaggcgctg ccgagacaat tcgcgaaaat aatgccctcg gtgccgtttg tgccagagtg
    23401 tgcccgacgg agaaattatg tcaaagcggt tgtacccgtg ccggtgttga tactcccatt
    23461 gatatcggtc gcttacagcg ttttgttact gattttgaac aacaaaccgg gatggaaatt
    23521 tatcagcccg gtactaaaac gctcggcaaa gtcgcgatta ttggcgcagg tcctgccgga
    23581 ttgcaggcca gtgtaacact gacaaaccag ggttatgacg tcacgattta tgagaaagaa
    23641 gcgcaccccg gtggctggtt gcgtaacggt attccgcaat tccggttacc gcaatcagtg
    23701 ctggatgcag agatcgcccg tattgaaaaa atgggcgtga ccattaagtg caacaacgaa
    23761 gtcggtaaca cactcaccct tgagcagctg aaagcagaaa accgcgcggt actggtcacc
    23821 gtggggttat cgagcggttc cgggttatcg ctgtttgagc atagcgacgt tgagattgcc
    23881 gtcgacttcc tgcaacgtgc acgacaggcg caaggcgata tcagcattcc acaaagcgca
    23941 ttaattatcg gcggcggtga tgttgcgatg gacgtagcca gcacgctgaa agttctcggc
    24001 tgtcaggcgg taacctgcgt agcgcgtgaa gagttagatg agtttccggc aagcgaaaaa
    24061 gagtttgcca gcgcccggga gctgggcgtt tcgatcatcg atggattcac gccagtagcc
    24121 gtggaaggca ataaagtcac gtttaagcat gtacggttac cgggcgaact gacgatggcg
    24181 gcagataaaa ttattctcgc cgtcggtcag catgccagac tggatgcctt tgcggagtta
    24241 gagccgcagc gtaacaccat caaaacacaa aattatcaga cccgcgatcc gcaagtcttt
    24301 gctgctggcg atattgttga gggtgacaaa accgtggtct atgccgtgaa aaccgggaaa
    24361 gaagccgccg aggcgattca tcactattta gagggagctt gctcatgtta acgaaagatc
    24421 tttcgattac tttttgcggc gtgaagtttc ccaacccgtt ctgcctctct tcttcgcctg
    24481 taggcaactg ctatgagatg tgtgccaaag cctacgacac aggttggggc ggcgtggtgt
    24541 ttaaaacgat cggctttttt atcgccaacg aagtctcgcc gcgttttgat catctggtga
    24601 aagaagatac cggttttatc ggcttcaaaa atatggagca gattgctgaa catccgttgg
    24661 aagagaatct ggccgccctg cgtcagctga aggaagatta cccggacaaa gtattgatcg
    24721 cttcgatcat gggggaaaat gagcagcaat gggaggagct ggcgcgcctg gtgcaagaag
    24781 ctggcgcgga tatgatcgag tgtaacttct cctgtccgca aatgacttct catgcgatgg
    24841 gtagcgatgt cgggcaaagc ccggagctgg tagaaaaata ttgtcgggca gtgaaacggg
    24901 gttccacgct gccaatgctg gcgaagatga cgccgaatat cggtgatatg tgcgaagtgg
    24961 cgctggcggc gaagcgcggc ggcgcagatg gcattgcggc gattaacacc gttaaatcca
    25021 tcaccaatat cgatcttaat cagaaaatcg gtatgccgat cgttaacgga aaatcgagta
    25081 tttccggata ttccggtaaa gcggtaaaac cgatcgccct gcgcttcatt cagcagatgc
    25141 gcacccatcc agaactgcgc gatttcccaa tcagcggtat cggcggcatt gaaacctggg
    25201 aggatgcggc tgagttttta ttgctcggcg cagcaacgtt acaggtgacc accggcatca
    25261 tgcagtacgg gtatcggata gtggaagata tggcaagcgg gttgtcgcat tatctcgccg
    25321 atcagggatt tgattcgctg caggagatgg taggtctggc gaataacaat attgtcccgg
    25381 cggaagattt agaccgcagt tatattgtct atccccgtat caatcttgat aaatgtgttg
    25441 gctgtggacg ctgttatatt tcctgttacg acggcggtca ccaggcgatg gaatggagcg
    25501 agaaaacccg cacaccgcat tgtaataccg agaaatgtgt gggttgtctg ctttgtggtc
    25561 acgtctgccc ggtgggttgt attgagctcg gggaagtgaa gtttaagaaa ggcgagaaag
    25621 aacacccggt aacgttgtaa aaccagagac gcatccggca tttggcaccg attgccggat
    25681 gcagcgcatc cggcaatatc cacaatcaca tcacttctta cgcgcgtatt tcagtgaatc
    25741 cagcgctacg gcgaagataa taatcgcccc tttgatgatg tactgccagt atgggtttac
    25801 gccgatatac gtcaggccat agttgatgac ggtaaaaata attaccccgg tcaccacgcc
    25861 aatcaccgtc cccacaccgc cgctgaacga tacaccgcct accacgcacg ccgcgatagc
    25921 atccagctca tacataaagc cgaggttgtt ggtggcagag ccgatacgtc cggcttctaa
    25981 catcccgcca aaggcataga acacgccaga caacgcgtag atcatcagca ggttcaggcc
    26041 gacgttgaca ccagatactt ttgccgcttc cgggttaccg ccaatggcaa aaatgttctt
    26101 accgaagcgg gttttgttcc acaacaccca gacgaacgcc accgcaatca acgcgtagaa
    26161 ggtgatgtaa gagagacgga aactccccag cgcgacaaag ccctgagcaa aggtagagaa
    26221 gccactgtca aaaccagaaa ttggcgacgc cccgacaaag tcgtaataga gcgagttgat
    26281 tccatagacg atgatcatcg tgccgagcgt ggtaatgaac ggcgtcacgt tgagataagc
    26341 gataatcaga ccgttgatca aaccgatcac cgcaccaatg gcacagacaa tcagaataac
    26401 cagcgcaatc ggcatcgtcg ccatttccgg gaacactttg ttggcgttat ccatggactg
    26461 caataatgtc gcagccacca ctgccgccag ccctacctga cgaccagcag aaagatcggt
    26521 cccctgggtg acaattaacc ctgccacacc gagcgcgata ataatacgca ccgatgactg
    26581 ggtgagaata ttacttaagt tcaacagact taaaaatgtt gggtcctgga aaataataat
    26641 cgccagcaaa actaaaagaa cgacgtaaat accgccctct ttcaggtaag taagaaaact
    26701 tttcttattt aacgcactca tgggaagccc ctaatcttaa aggtgcaaag acgcaagacg
    26761 cagaatttcg ttttgcgttg ttgtttttgt atcgacaatt ccggaaacga gaccattgct
    26821 catgaccaga atacggtctg ttatccctaa caactcaggc atttcagagg agataataat
    26881 aatccccttg cctttcttcg ccagttcggc aattaactga taaatttcaa acttcgcccc
    26941 gacatcaata ccgcgcgtcg gttcatcgag cattaatatt tctggttgcg ttagtagcca
    27001 gcgaccaata atcacctttt gctgattacc accggagagc gaaccaattt gcgtccgatg
    27061 acccggcgtt tttacccgca tcgaatcaat cacccactgg gtatcgcttt tcatccgcga
    27121 gttatccagt aaaccaactt tatttttgta gttgcgaata ttggaaatta acgagttaaa
    27181 actaatatcc agataggcat aaattcccgt tgagcggcgc tcctcagtta ccagtgcaaa
    27241 tccgtggttt atggcttcgt tggcattatg gttattgatc tgtttgccgt gcaacgtaat
    27301 ggtgccagcc gatttctcgc gaataccaaa taacgtctca acaatatcgg tacgtttcgc
    27361 ccccaccagc ccggcaatac cgaggatctc ccctttatgc agatcaaacg agacatcgcg
    27421 aatcgacggc tggcgcagtg acgttaggtt acgcacctcg aggatgactt cgcccggctt
    27481 gttttctttg tcaggaaaac gctgattaag agaacgccca accatcatgg cgatgatctt
    27541 gtccatcgtc agtcctgcca gcggttcggt ggcgatccac tgaccgtcgc gcaatacggt
    27601 aacttcatca cataactgga agatttcttc cattttatgc gagatataaa caataccgca
    27661 gccgcgctct tttaatttac gaataatagt gaacagatga ttgacctctt tttcggttaa
    27721 cgaagaagtc ggttcatcca taatcacaat tttcgcgtta taggaaaacg ctttggcgat
    27781 ttcgatcatc tgcatttgcg aaacggataa tgtgccgacg cgcgcacgcg gatcgatatc
    27841 aatatccagt tcatcaaaaa tcgctttggt ttcgcggtac attttgtcct gatcgacaaa
    27901 catgcctttg gtgggatatc gccccagcca catgttatcc atcaccgaac gttgtaatac
    27961 caggtttaac tcctggtgta ccatcgaaat accattttcc agtgcctctt tggcggaatg
    28021 gaaatcgatc tctttaccct ggaataaaat ggtgccggag tctttttgat aaataccaaa
    28081 caggcatttt aataatgtcg attttcctgc accgttttcc cccattaatg catggataga
    28141 atgtggccgg acttttaaat taacgttatc aagtgcctta acaccaggaa aggacttgtt
    28201 gataccgctc atttccaaca agtattcccc ggaagacgga gtcgttgagc tgaccatata
    28261 attttacctt gttggccata caataagggc gcagtaaacg actgcgccca atcagtctta
    28321 tttcttgctg aattcagcca ggttgtcttt atctacgcca acataaggta cgcggaccac
    28381 tttgttgtcg attttccagt tggtgccatc agccgcacct ttgccatcgg ccaggttttt
    28441 cgccagatca aaggtcgctt tcgcctggtt gttagcatcg ttcagtactg tgcccgccag
    28501 tgcaccggat ttcaccagcg ccagcgcttc tggcagcgca tcgacgccaa acaccggaat
    28561 gctggacttg ttgtgtgctt tcagtgcttc taccgcgccc attgccatcg catcgttgtt
    28621 ggcgataacc acttcgattt tgttggcgtt cgggccagac agccaggcgt ccatcttatc
    28681 tttcgcctga gcggtatccc acatagcggt atctaactgt aactgttcag ttttaatgcc
    28741 tttgtcgttc agctctttaa tcacgtaagt ggtacgtgct tctgcatccg gatggcccgg
    28801 ttcacctttc agcaatacga attgaatctg accgtctttg ttcagatccc aaccctgatt
    28861 cgccgcccag tgtttagcaa tcaaatcgcc ctgaataatg ccggactctt tggaatcagt
    28921 gccaacgtag taggctttgt cgtagctatc cagcgcctta cgagacggtt ctttgttgaa
    28981 gaaaaccacc ggcacgtttt gcccacgcgc tttctcaatc accgtacccg cagctgccgg
    29041 gtcaaccagg ttgattgcca gtgccttcac ccctttcgcc agcaatacgt cgatctgatc
    29101 gttctgcttg gactggtcat tctgagaatc attcatcagc agctgaacat ctggcgcggc
    29161 tttcgcatct tgctcaatag ccttgcgcac tacagacata aagttatcgt cgtacttata
    29221 gattgttaca ccaatgcgag tatcagcagc gtgtgcagcg gcaccgaata acatgctggc
    29281 catcacagca gacagggtta acaccttctt attcatggta tctccggttt ttcttatgca
    29341 gggtagtgct tgagataaat gctcggcggg gcagtagagt taatgaagtg ttactgaacg
    29401 ccgaagctca ctttttaaaa ttcgttcttc catgcccggt aacgctccag aaaacggctt
    29461 taattgttgt tatgacgcta ttacctcggt gaaagtgatt attcactgtt acatacgggt
    29521 tacaacgtta aaacggtgca atcatagcta tcacattgtt aagatactgt gaaatcactc
    29581 acagattgaa agcggttaca tcgcctgatt tgttgagtta gtgatcgtcg ccgcattctg
    29641 gcgtgttgcg acagaatggc gacgcactaa cgtcggcata aaacagtggc tagcacgagg
    29701 atcaatattg cctgctgccc cctgcaaggc cagttcggtg gctaatttcg ccattgaagc
    29761 aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa cgggcgatgg gaatatcatc
    29821 gaaaccgatg attgagagat gtaacggaat cgcaatgcca ttatctttta atgctgtcag
    29881 tgcgccagca gccatattgt cgttataagc aaatacagcg gtaagttgta gattgcgccc
    29941 cagcagttca accatcgccg cctcaccgcc cggcatgtcc ggcgtaccag tgccaatcca
    30001 gctttccggc ggaataatat cctgctcttt caacgcactc atccagcctg ctttacgcat
    30061 ggcgtcatct tcaatgccgt gactggaaga aagataacca atacgttgat gaccgttatt
    30121 cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta tccaggcaaa cgcaacgatg
    30181 ggcgtacccc ggcacaacgc ggttgattaa caccataccg ggaatgttat ccataaattg
    30241 cgccagttca tcgtcactca atgcttttga gtgaacaatc aacgcattac aacgctggcg
    30301 aattaacacc tcaatggcgt gacgctcttt ttccgcttca tgatagctat tgccgattag
    30361 cacgtatttc tgatgctgct gagcgaccag atccaccgct tttaccagcg cgccgaaaaa
    30421 cgcatcagaa acgtccatca ccaccacgcc aatggtgtcg ctaacctgag ttgccagcgc
    30481 ctgggcattg gcgtttggcc gataatccag ctcactcacg gctttcatta ctgcttcacg
    30541 cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg gaaaccgttg ccacagagac
    30601 gccagcctga cgcgctacat cacgaatggt gatcatattc actacctgtt atgagattgc
    30661 agtaaatgac tgcttgctgg cggctatttt gtcagcactg aatacaggac ttcgtgaatc
    30721 gagtcacagc aatggaaacg gttacagccg ttttgttaac gaatgtgatc cagatcgtta
    30781 tcttgatgtt ttagatattg ccgttccggc agcacgcaaa gttaactgac gccagagcca
    30841 ttccaccggc ccctggcgga agtaacacag ccagataaca gagaagagga tattcgccag
    30901 ccataccgga ataacaaacg ccagcagctc caggcggtca aaatgcataa acaaaccgag
    30961 gtggtaaaaa agcatggtac aaatcagcgt ttgcaataga tagttggtca gcgccatccg
    31021 tccgacgcag gcgatcgcaa gcaccagctt aaagcggctc aattgcggcc agaagccata
    31081 aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc agttcccgcg gcatttgaag
    31141 taagaaggca caccagcgat acgcccagtc cagctgccat tgcagggcga tggcaggaag
    31201 gttaatggtc accccaatcg ccaccagcac aaaaccagta cgacgatagt gacgtaagct
    31261 gaactgccct ttcagccagc cgctgcgcat cagcgctgca ccaatgagca tcatcccagc
    31321 cagttgccag ccatactgtg cgcccagtgc cagtaaactg ttgccaacac catcggcacg
    31381 attactgatc gcttcaacgc cgccgtgaag cttccagtat ttttcataca aaatagccga
    31441 tgcatccggc gtccaggcgc ggctggtctg gctgtcggaa atcagcccca ataacagcaa
    31501 aacgccaagc cccaccagat aaagcatgac gcctgtatta aacaggcttt ttaccgatgg
    31561 cgcatcgcgc accagccgcc agcagattaa gcccaccagc ccgtaagcca gcagaatatc
    31621 gccgtcccag aacaataaac cgtgaataaa gcccaataag actaacagcg ttaaccgcga
    31681 ctggatccag cgtctgccac ggggcagcaa catttgcagt ccagcaccaa acagcagcgc
    31741 aaaaagcgtg aggaatttca cctgggcgat gagatcaaga aatgcccagg tccatgcatc
    31801 ctgcggcgta atagcaccgt accaggcggg attgagataa gccgcctttg gtagcccaaa
    31861 ggcgctgatg tttaatagca ggatccccag aatggcgacg ccgcgaacaa aatcgagcgt
    31921 gacgttgcgc tccatggttc ctgcctttta atcagttgtg atgacgcaca gcgcgcagaa
    31981 actcgtggcg cgtattctga ctggatttga acaatccacc aagagaggtc gttgttgtgg
    32041 cactggttgc atcgcggatg ccacgcgcct tcacgcagta atgcaccgcg tcgatcgaga
    32101 cagccacgtt attggtgccc agcagcgttt gtagcgcaat aagaatttgc tgcgtcagac
    32161 gttcctgcac ctgcggacgc tgggcaaaga actgcacgat acggttaatt tttgacagac
    32221 cgatcaccga atctttcggg atataagcca ccgtcgcttt gccatcgatg gtaacaaaat
    32281 ggtgttcaca ggtactggtc agagtgatat cgcgcacggt gaccatttca tcgaccttca
    32341 ttttgttttc aatgagggtg attttcggga agttggcgta atccagaccg gagaaaattt
    32401 catcgacata cattttagcg atgcgatgcg gcgtttccat caaactgtca tcagccaggt
    32461 cgagattcag cagctgcatg atttcggtca tatgaccagc aataaggctt ttgcgcgttt
    32521 cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc cagtcctcgc gcaactaacg
    32581 cttcatgaac cagggccgct tctttactga gtgatggcat ttatgatttc tcctgcaggt
    32641 gtgacgcctc cgccctgcgt gggggcaaag ttattaagct gatttacagc ttgattattg
    32701 tgcgtgaggc ggcgcacata atccagtatt cacagcgata attattgtaa ttgccgctgc
    32761 ctttcatcag cagatgttaa aacatcgtta tgcaaatacg gaagtgaaag ttactcacag
    32821 cacattgaat aaacggtatg atgaagaaat tgcaaacaac acaacaagga gccacgcatg
    32881 gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc aacgctggcg acacgactcc
    32941 agtaccttaa actgtccgat gacgttcagt atctttctcc caccacctcg tgatcacacc
    33001 ccgccaccag tgctgtactg gctttccgga ttaacctgca atgacgagaa cttcaccatc
    33061 aaggcgggtg cccagcgcgt ggcggcagaa ctggggattg tactggtgat gccagacacc
    33121 agcccgcgcg gcgaacaggt tgccaacgat gatggctacg atttaggcca gggcgcgggg
    33181 ttttatctta acgccacgca accgccgtgg gcgacgcatt accggatgta tgattatctg
    33241 cgcgatgaat taccggcgct ggttcagtcg caatttaatg tcagcgaccg ctgcgccatt
    33301 agcggtcatt caatgggtgg tcacggtgcg ctgattatgg cgctgaaaaa tccgggtaaa
    33361 tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt gcagcgtccc gtggggaatc
    33421 aaagcgttta gcagctattt aggtgaggac aaaaatgcat ggctggaatg ggacagttgc
    33481 gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga cgcttatcga tcagggcgat
    33541 aatgatcagt ttcttgccga ccagttgcaa cctgcggtac tggcagaagc cgcgcgccag
    33601 aaagcgtggc cgatgacgct gcgtattcag ccgggatatg atcacagtta ctacttcatc
    33661 gcctctttta tagaggatca cctgcgcttc catgcgcagt atttactgaa gtgaaagtcc
    33721 gcccggttcg ccgggcatct tctcatcaga agcgataatc cactgccata aagtaacgac
    33781 gtccgtcttc gttatagctg tagtcgtcac gactgagatc cttgtcgcca aggttcagca
    33841 cgcctgcgcg cagtttgacg tctttagtca cctgccaggc cgcgccggta ttccagatgg
    33901 tataaccgcc cggtgtttta gccgtcgcgc tgtcggcgcg tttctgcccg gtatagtgac
    33961 cagaaacata gaatgaccag tcttccagcg ccagcggttt ccagtccagc gtaccgttag
    34021 cagtatggaa cggcagatcg gatagcggtt tgttttcgcc gttgctgaca tcacgaccat
    34081 cgttgtaggt gtagttgatc gacagtttcc attcatcgtt gaacggaatt ttcagttcgg
    34141 tttccacgcc ctgaatacga gctttgttaa cgttgtagta gctaaatacc ggtatgcgcc
    34201 gtccgttagc gcccgtctca aaaccaacaa agttttggta gcccggtgca gcgttgacgt
    34261 cagacgtacg gctgatgctg atacgatctt tcacatcgtt acggaaaacg gtaacgctgg
    34321 attcaacccc ttccagccag ccttcttcac ccatgtagta aagccccagc tcccaacttt
    34381 cgctggtttc tggtttcaga tccgggctac ccacaatctt acatgcgcca cggcaggaat
    34441 tgctcgtcca gtcagggcta agttgcaaca gagaaggtgc tttaaatgcc gtcgcccagc
    34501 cccctttcac cgttacggtg tcggtggcgt tataaaccag gtaggcacgc ggactccagt
    34561 gttcaccgta ggtttcgtga tcgtccatac gcacgccggt cgtcagcgcc agcggctcga
    34621 agatccgcca ttcatcttcc acaaacagcg cgtactggct ggcagacgtt ttggagctgg
    34681 ttcccccggt caggttcacc gcatcgctaa gtttgtcgtg acgccattca ccgccaaccg
    34741 tgagaaactg attaattgcc gtcagcggca acgtgtattt gccgtcgacc gtattgcttt
    34801 cggaagttat caggctgctg ttgccagggt ttttgttctc gactttctca ccgtagtatt
    34861 tcagttcgct ggtgccgtaa tcccaacgcc cattatggct gacggagtag ttctggcgtt
    34921 ccaggcggtt tttgtccagc gagtcggaat cacgatcctg acggtcgaaa ccgtatccgg
    34981 cagtaaaatc gtgattttga ttcggtgtcc aggcaaattc gacattgccg tcgcggctgg
    35041 agaatccttc aatacgcggc gtttctccgg tatcggtggt cgttgagttt tgcgggtcat
    35101 ccttttcacg ttttgccagg ctgccgtaag ctttcattcc cagcacacca tcaattaatg
    35161 gtccactggt aaagaactga ccgttatagg tatcaccgcg atcgcgatgt tcctgaatgg
    35221 tggtatcgac ggtaacggta cccgaccatt tctgaccgat ttttttggtg atgatattca
    35281 ctacaccgcc gagcgcatcg gagccgtata gcgacgacat cgggccacga accacttcaa
    35341 tacgttcgat ggaatcgacc gggatccagt tcagatcgaa atcattgtgg cggaagacgg
    35401 cattgcggga gttaacgcgt ttaccgtcga cgagaatcag ggtgtagctg ctgtccagac
    35461 cacgaatact aacgccctta cggttatccc cttcgttcgt cagttgtacg ccaggcactt
    35521 ctttgaggac atccttcaga ttctgtaccg gttttcgctg caggtcttcc tgggtaatga
    35581 cgctgatact ggcgggtgca tctttgaggt tttgttcaac ggaagatgca gtgacaacca
    35641 tcgtttcgcc atcatcatcg accgctaaca caggccatgc acaagaaata gcggacaaac
    35701 acagcccgac ccgtacgaaa gggttcaacc taaacattcc atatctccat gaggtaacta
    35761 cgaaaataaa atgggttatc gctcacatct tcttcacgtc cccttgcgta cggcagcatc
    35821 gcggtggact tatttgtatg caggtgatca tcccagaaag ccggaacagc ctctgatttg
    35881 tcagcctttt ttgattgcgg cgtaacgata atgcaaacga taacaattat caatccaaat
    35941 gttaaatttt atatccgcat gatttgtcag ggaaatggaa ttgaaaaagc cctctcggtt
    36001 gagagggctt agcaaggaag ggaggaaact tatttcttat cgttctgcgg gaacttcatt
    36061 tcgctgtagc gtacgaagtg agttccttta atcagcttgt agccgaacca aataatcagg
    36121 aacagcggga taccaatata cgttgccgct acgccgcccc agtcaatggt atctttcagg
    36181 aacgcttcgt agttctggcc caaagtgata atcagacaca gaatgaatgc gaagatcggc
    36241 cccagtggga agaaacctga acggtacggc agatcgttaa tgtcgtgtcc ctgcaatacg
    36301 taaccgcgac ggaagcgata gtggctaatg gcaatcccca gccaggcgat aaaacccgtc
    36361 atcccggagg tgttcagcag ccacaggtat accgtctggt tgccaaacat ggaggtcagg
    36421 aagcacagac cggcaatcac cgtcgtcgca tacagcgcat tacgcggcac gccaccacgc
    36481 gacagtttag cgaaaatgcg cggcgcttta ccgtcacacg ccagggtgta cagcatacga
    36541 gtagacgcat acataccgga gttacccgct gacagcaccg ccgtcagaat aactgcgttc
    36601 atcaccgccg ccgcagagag cagacccgcg tgctggaaca ccagggtgaa cggactaacg
    36661 ctgatgtctt taacatcgtt acgcagcagg ctcggatcgg tgtacggaat aatcaggctg
    36721 ataatcagga tcgcgaacac atagaacaac aggattcgcc agaacacctg acgtaccgcg
    36781 cgtggaatgt ttttcgccgg atcttcggac tcgcctgcag caataccgat cagctcggtt
    36841 ccctggaaag agaagccgac aatcatagct acgccgatca tcgccgcaaa accaccagca
    36901 aacggcgctt cgccgattgt ccagttgctc cagcccgcag gctgcgcgcc tttgaagata
    36961 ccgataatca tcagcacgcc aacgatgata aagacaataa ctgtcgtgac tttgatcagt
    37021 gagaaccagt attccgcttc accaaagcca cgaactgaga tgtagttcag caggaagata
    37081 acgccgagga acaacgcact ccagatccag cccggtgtat ccgggaacca ccagctcatg
    37141 accagctgag ctgcaaccag gtcaacggcg atagtcaccg cccagttgta ccagtagttc
    37201 cagcccagcg cgaagccaaa gccttcttca acatagttct gaccgtaagt ggcaaacgaa
    37261 ccggaaaccg gcatatatgc agctagttca ccgagactgg tcatcaggaa gtaaaccatc
    37321 aggccaatca gcatatacga gagcaatgcc ccgcccggac ctgcctgaga aatcgttgcg
    37381 ccagaggcaa caaaaagacc tgtaccgatg gaaccgccaa tggcaatcat cgtcaggtga
    37441 cgcgccttta attcacggcg taagcccggc gcttctgtgg ttttagtttc ggaaaccata
    37501 caaaaatgct atccatctta aaaatgaggc gcgattgtag cagacgattg gcaatccttc
    37561 cggcagaaat acacggttat aagacacctt catgatcgcc cagggattat aagtaaagca
    37621 gccaatctct tttctggcga atgcatgctg agggcgctgc gtcacgaaat cgacacagca
    37681 ccagcatgtt cttgtacagc aacttaacgc ggtacattcg cgggatcgca atagtccaga
    37741 aagcgccgta gcgcgttgga aaggtgtttt tgccgatgat gtatacgcca caacgtacgc
    37801 atcaggcgcg ggagagggac cgcaacttca cttaatgtgc ctgcctgcaa ttgatcttta
    37861 atcacccgtc gcgacaggca actaattccc aacccatgac gcaccgcatg tttgattgcc
    37921 tcggagttac ctaattccat cgccatctca aacttcggta aatgtgacag caacagataa
    37981 tcgacaatct cccgcgtgcc ggaaccgcgt tcacgcagga tccacggcgc agcggccagc
    38041 tgttctaagg tgaccggacc acgagccaac ggcgaagtcg gcgcggcgaa aaccaccagc
    38101 tcgtcttcca gccacggttc agaaatgatt tcagtgctgt ggcacggtcc ttcaataaag
    38161 ccaatatcaa cgcggaaatc cagcaccgct tgcatcacgt cctggctgtt cccaacgcta
    38221 agttcaatcg gcaactgcgg ataatcatgg cgataacggg cgataactgc aggcagaatg
    38281 tagttaccga tggtactact ggcatagata cgaatcgcgc cgttgtcttc gcgaaacagt
    38341 tgttcgattt caaccgcctg ttcaagcaat gccaacgcac gcggatagag cagccgcccg
    38401 tgttcattaa caaccagtct tttccccacg cgatcaaaca gttgcacgcc aagctgccct
    38461 tccaggtcgg tcaaggctgc gctcactgct gattgcgaca acgccagcat caccgacgcc
    38521 tgggtggttg atccactttt caatacttct gcaaaaactt ccaactgccg gagggtgatg
    38581 tgcatagtcg cttaccactt ataaagatta attataaata tataatcaat tttattttta
    38641 aaccagttag tcgtaacctt atgcccggtt aaaggagagg gttatgacga atatcacctt
    38701 acagaaacaa catcgtacac tgtggcattt tattccgggg ttagccctga gtgcagttat
    38761 caccggggtc gccctgtggg gtggttccat tcccgcggtt gcgggtgccg ggtttagtgc
    38821 cctcaccctc gcaatcttgt tggggatggt tttaggcaac accatctatc cgcacatctg
    38881 gaaaagctgt gacggtggtg tgctgtttgc caagcaatat ttattacgtc tgggtatcat
    38941 tctttatggc ttccgtctga cgttctcgca aattgccgat gtcggtatca gtgggatcat
    39001 cattgacgtg ttgacgctgt ccagtacctt cctgctggct tgcttcctgg ggcagaaagt
    39061 gtttggtctg gataagcaca ccagctggtt gatcggtgcc ggtagcagta tctgtggtgc
    39121 tgccgcggta ctggcgactg agccggtagt gaaagcggaa gccagtaaag taaccgtggc
    39181 tgttgcaacc gttgttatct tcgggaccgt cgctattttc ctctacccgg cgatatatcc
    39241 gctgatgtct caatggttta gtccggaaac tttcggtatc tacattggtt ctactgtgca
    39301 cgaagtggcg caggtggtgg cggcaggtca tgccatcagc ccggatgcgg aaaacgcagc
    39361 agttatttcc aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc tgctggcggc
    39421 gcgtgttaaa cagctgtctg gggcgaacag cggcgagaaa agcaaaatca ctattccgtg
    39481 gtttgccatc ttgttcatcg tagttgccat ctttaactcg ttccacctgt taccgcagag
    39541 cgtggtgaac atgctggtaa cgctggatac cttcctgctg gcaatggcga tggcggcgct
    39601 gggtctgacc actcacgtca gcgcgctgaa aaaagccggg gcgaaacctc tgctgatggc
    39661 actggtactg tttgcctggc tgattgttgg tggtggtgcg attaactatg tgattcaaag
    39721 cgtcatcgca taaaccacta catcttgctc ctgttaaccc gctatcatta ccgttttcct
    39781 ccagcgggtt taacaggagt cctcgcatga aatacattgg agcgcacgtt agtgctgctg
    39841 gcggtctggc aaatgccgca attcgcgccg ccgaaatcga cgcaaccgcg tttgccttgt
    39901 tcaccaaaaa ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc atcgatgaat
    39961 tcaaagcggc ctgtgaaaaa tatcactaca catcggcgca aattcttccc cacgacagct
    40021 atctgattaa cctcggacat ccggtcactg aagctctgga aaaatcgcgc gatgccttta
    40081 tagatgaaat gcagcgttgc gaacaactgg ggctttcttt gctcaacttc caccctggca
    40141 gccatctgat gcagatttca gaagaggatt gccttgcgcg tattgccgaa tccatcaaca
    40201 ttgcgctgga taaaactcaa ggtgtaacag cggtgattga aaacaccgcc ggtcagggca
    40261 gtaacttagg gtttaaattc gaacatctcg cggcgattat cgacggcgtg gaagataaat
    40321 cccgtgtcgg cgtctgcatt gatacctgcc atgctttcgc tgccgggtat gatttgcgta
    40381 ctccagccga atgcgaaaaa acgttcgctg attttgcccg tattgttggc tttaagtatc
    40441 tgcgcgggat gcaccttaac gatgcgaaaa gcacctttgg cagccgcgtt gaccgccatc
    40501 atagcctcgg tgaaggcaat atcggtcatg atgcgttccg ctggatcatg caggacgacc
    40561 gtttcgacgg cattccgctg atccttgaaa ccatcaaccc ggatatctgg gcagaagaga
    40621 tcgcctggct gaaagcgcaa caaactgaaa aagcggtggc ctgatcgatg aacgacaggg
    40681 aaaagcagat tcttaaaatt ttacggcgta acccgctgat tcaacagaat gaaatcgccg
    40741 atattctgca aatcagccgc tcacgtgttg ccgctcatat tatggatttg atgcgcaagg
    40801 gtttgattaa aggcaaagga tatatcctta ccgagcagga ttactgtgtg gttgtgggtg
    40861 caatcaatat ggatattcgc ggaatggcgg atatccgtta cccgcaagca gcctctcatc
    40921 caggttctgt tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat aacctggctc
    40981 tgcttggtcg ggatgtgcat ttaatatcag ctattggcaa cgatttttac ggcgagacat
    41041 tactggaaga aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt ctgcatggac
    41101 acagcactgc aacttatctc gctatcgcta ataagcagga agaaactatc ctggcaatca
    41161 atgacacaca tattctgcaa cagctaacgc ctcaactgct caatacttca cgggacttga
    41221 ttcgccatgc tggtgtggtg ctggctgatt gtaatctcac acctgaagcc ctggagtggg
    41281 tctttaccat tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa tttaaagcga
    41341 acaaagtaaa aaactggtac agccggatcc acactctgaa accaacacaa aatgaactgg
    41401 aaattttgtg ggggcaaccg attaaagacg acaacgatcg catcagagct gtaaattcgc
    41461 tgcatcaaca aggtgttaaa cgcatttttg tttatctgaa agatgaatct gtattttgca
    41521 gcgataagga tggagaacaa tttttgctga ccgcgccagc gcatacgaca gtagacagtt
    41581 ttggtgctga cgatggtttt atggcgggcc tggtatatag ctttcttgaa ggatacagtt
    41641 tccgcgacag cgcccgtttt gcggtagcct gcgcggcaat ttcgcgcgcc agcggcagct
    41701 taaacaaccc taccctgtct gccgataacg cgctttcatt agtgccaatg gtgtaacaat
    41761 gttgccggat tcgacgcttg accgcatctt atccggctca taaattgcgc actacgctaa
    41821 accaataaag aatcctgcaa tagtagcact catcaggtta gaaagtgtcg ccgccgccag
    41881 cgcgcgtaaa ccaagttggg cgatttccgg cgcacggtgt ggcgcaaccg cagaaaacgc
    41941 ccccaccacc accccgatag aaccaaagtt agcgaaaccg cacaacgcga aagaaataat
    42001 cgccacggtt ttagcatcca gagtgccacc cgtttgcaga tatggcgaga aattgagata
    42061 agcgacaaat tcattgatcg ccagcttctg cccaatcaaa ctcccggcaa gatttgcatc
    42121 actccagtca acccccatca cccacgccaa tggggccaac aggtaaccta aaatggactc
    42181 cagcgaggca tgtgcaaaac caaaccagcc gccaacgccg ccgataatac cattaattaa
    42241 cgcaatgatg gcgacaaatg ccataacaac tgtcgctaca ccggcggcga ttttcagccc
    42301 ggtcattgcc cctgtcgcag cggcttcaat aatgcttttt ggcggtgttt cggtgaaaga
    42361 gaggttatta aaggaaacct gcgaagattc cgtagccggg cttaacaggc gggcaaacaa
    42421 gatcccgcct gggatcgcca ttaacgatgc cgccagcaaa tattccacag gtacgcccag
    42481 ggcggcgtaa ccaatcattg tcgaaccagc aatcgaggcc atgccactac aaatcgctgt
    42541 aaataattca ttgcgattca gacgatcgat aaagggtttg acgattgccg gaatttcgtt
    42601 ttgcccgagg aaaatggtag tgaccgcgac gaatgactcg atcttgctga tatttaacgc
    42661 tttctggaag ataccgccga gaatgcgaat taaaatcccc atcacaccga tgtagtagag
    42721 aatactcacc agtgctgtaa cgaagataat tgccggtaat actctgaaac caaagataaa
    42781 tcctgcgcca tcaaacagcg tgtccatttt cggcccgacc agcgaaccga agataaatgc
    42841 gctacccgcg tcgctgtacg ccatcacttt atgcacgcca aaagcgactt tttcagcgac
    42901 ccaacgccct ggcggtaacc aaagcataat gccgccaatc acaacctgta acactaacgc
    42961 cgcgccaacg gtacgcaggc tgatcttctt cttgtttact gacagcaaaa atgcaattgc
    43021 cagcaatacc accattccca gaacacttct catgacatcc atagtgatta tctcttcatg
    43081 tcatattggg cgacggcttt ctgcccatca gaaatagccg ggcctgcatc ttgcgctatc
    43141 ggcccggctt gttatcaacg cttaatgggt tttgatgtag ccgcgcacgc actcttcgac
    43201 taagccccag aaccagtcgg tatcaatagt gatgccgact ttggtattgg cgggcttgcc
    43261 aagaacgccc agctcgtcgc agacggtgcg cccatagcaa gggccactgt tgacgtccac
    43321 ttcgacgtac atctcctggg ttttaatgcc atcagggtta atcagataac cgatgcaggt
    43381 ggcgtcgtgc accgggccgc cagccaggcc gtagttttcg aactgcgttt tgagagtgaa
    43441 gttcatgatg tcgctgaaca gctctccggc agggccgcct gccctttcca tccgagcaat
    43501 cacgtccggg gtgcaaacgg tctggttggt gagatcgagg cccatcatca ctaatggaac
    43561 gccagaggtg aacaccacgc gggctgcttc cgggtcagca aagatgttga actcggcaga
    43621 tggcgtgaag ttgcctgtac cgtaagcgcc gcccattagc acaatttcac ggattttggg
    43681 caggatcgcg ggttgcatac gcattgccac cgcgatattt gaaagcggac caaccggcac
    43741 cagagtgata tcgccatcgc ttgccatcag ggtatcaatg atgtatttca ccgcgtgggt
    43801 actttctgcc tggcgggtta gcggctcgaa taccgggcca tccagtccgg tttcaccgtg
    43861 aatattatcg gcaacgattt gttgacgcat aatgggttgc ggcatccccg cgtaaaccgg
    43921 aacattaatc tccagtttct ggcaaacatt caggccatta attaatgttt tatcaagcgt
    43981 ctgattaccc gctacaatag tgatgcctaa taaatctatt gccggatgtt tcgctgccat
    44041 catcattgca atagcatcat catgacccgg atcacaatcc agaataattt ttctcttttc
    44101 cattgtttat ttcctctgtt tccagttgcg ttattttttc tacagcaaag aaaattcgac
    44161 cgggccgatg attgaatctt accaaccgcg tacgtatgct aaatatgaga aatctcatag
    44221 cggataaaca tcgtgaaaga aatccacaat aatgatctta agcagcaatt gatgagtgaa
    44281 tccgcgttta aggattgctt tttaacggat gtttcagccg atacgcggct gtttcatttt
    44341 ttagcgcgtg actacattgt gcaggaaggg caacagccgt cctggctgtt ttacctgacg
    44401 cgaggccgcg ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct gatcgatttc
    44461 tttgccgccc cctgttttat tggcgagatt gagttaatcg acaaagacca tgaaccgcgt
    44521 gcggtgcagg ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca ttaccgcccg
    44581 cggttattaa acgacacgct atttttacga aaactctgcg tcaccttaag tcataaaaat
    44641 tatcgtaata ttgtttcttt aactcagaat caatcatttc cgttagtgaa tcgcctggca
    44701 gcatttatat tactctcgca ggaaggtgat ctttatcacg aaaagcatac gcaagcggca
    44761 gagtatttag gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt cattcacgac
    44821 ggtttattaa ttaaaagcaa gaaagggtat ctaattaaaa acagaaagca gttgtcagga
    44881 ctggcgctgg agatggaccc ggagaataaa ttctccggga tgatgcagta aaaattattt
    44941 gcaatagcgc gattgccgga tgcaacactt aataagcgcg tacatccggc aatgaaactc
    45001 aagctaaacc aataaagaac ccggcaatgg tcgcactcat caggttggaa agcgttgctg
    45061 ctgccagcgc ccgtaaacca agctgggcga tttccggcgc gcgttttggc gaaatagccg
    45121 aaaatgcgcc aacgacaaca ccgatagaac caaagttagc aaaaccacaa agcgcaaagg
    45181 agataatcgc aatggttttc acttccagcg tgccgcccgt ttgcaggtat ggggagaaac
    45241 tcaggtaagc gacgaattcg ttaatcgcca gtttctgccc aatcaggcta cccgcaagat
    45301 tggcatcact ccagtccaca cccatgatcc acgccagcgg tgccagcaca tagccaaaaa
    45361 tactttccag agaggcattg gcgaaaccaa accagccacc aattccgccg ataatgccgt
    45421 tgatcagcgc aataattgcg acaaacgcca ttaccaccgt cgctacacca gcggcgattt
    45481 tcagcccggt catcgcaccg ctcgccgccg cttcgataaa gctttttggc ggcgtttcgc
    45541 tgaacgacag attttcaaat gtgacctgtg aaggctcggt ggccgggcta agaatacgtg
    45601 caaacaaaat accacctggg atcgccatca gcgatgccgc taacaggtag tcaattggta
    45661 cgcccattcc ggcataacca atcatcatcg aaccagcaat ggacgccatc ccgctacaaa
    45721 ttgcggtaaa caactcgttg cgattcatgc gatcgataaa cggtttaacg atcgccggga
    45781 tctcattttg cccgaggaaa atagttgtga ctgcgacaaa agattcgatt ttgctgatgt
    45841 taagagcttt ctggaaaatg ctgccaagga tgcgaatcag cagccccatc acgccaatgt
    45901 agtacagcag actgatgagc gcagtaacga aaataatcgc cggaagtacg cgaaaggcga
    45961 agataaaacc cgcaccgtca aacaggacat ccattttcgg cccaaccagc gaaccaaaaa
    46021 tgaaggcgct accggcatca ctgtaagaca tcactttatg aacgcctaat gccgcctgtt
    46081 ctactgccca ctttcccggt gggaagtaga gcatgatgcc accaatagcg atttgcagca
    46141 gcagtgcggc tccaaccgtg cgcaaactga tgctcttttt attcactgac aacagaaatg
    46201 ctattgccag taacaccacc atccccacaa cacttctcat tatatccata atgattttcc
    46261 cttcatgcca gtaaacccgg cgtcagcgcc aggttttggt atgcttgatg agtacgggcg
    46321 acggctttct gcccgtcaga aattaacccg cgagacgctg gtattctttg gcaatttcac
    46381 tcgccagaat ggcgttgttg aacaccagct ggatattgga tttcaggctg tcaccgccgg
    46441 tcagctcagc aacgcgcgcc agcaggaacg gtgtactttc tttaccaata acgccctgct
    46501 cttcggcttc ggcgacggct tgatctatgg ctgcattgat agattcctcc ggcatcgcaa
    46561 actgttccgg gatcggattc gccactacca ggccgccgtt caaaccggtt tgccatttca
    46621 ctgccattgc acgagcgatt tccgtggcac tatcaaggcg aatactgact tcaaatgggc
    46681 tggtacggca gaaaaacgca ggcagcgctt ttgtctgata gccaattaac ggcacaccga
    46741 aggtttctaa atattctgtt gttaatccta aatcgagaat agatttcgcc ccggcacaaa
    46801 caacggtgac attagtattt gccagttctt gcaaatcggc agaaatatcg aaggtatgtt
    46861 ccgccccgcg atgcacacca ccaattcccc cggtggcaaa tactttaatt ccggcaagcg
    46921 ccgcaataat catcgttgaa gccacagtgg ttgcgccatt ttttccggcg gcaacaacaa
    46981 aaggtaaatc gcgacgacta actttggtca cgttatgccc ttcacgaccc agtaattcaa
    47041 tttcttcttt gcttaaaccc actttcatca cgccgccaat aatggcgata gtggcaggta
    47101 ctgcgccctg tttacgaata gtttcttcta cttcaattgc ggtctgggca ttttgtggga
    47161 acggcatccc gtgagaaata atggtcgatt ccagcgccac aaccggtttt ttgtttttta
    47221 aagcgtcctg cacttccggg gaaatttgta ataattcagg ggaaattttt aattcagaca
    47281 ttctgcgttc tccactaacg atataacgtt ggcaatcgat aaatcggggt tattggtgta
    47341 ttcacaggag agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt cggcaaacgg
    47401 cattccgtct acccaacacg aagcaagtcc cgccatcatg gcatcgcccg ctccggtaac
    47461 attaataaca ttggttttga tcggggcaga ccagccactt tcaccgctga tatcactgta
    47521 ataaacgcca tcgccgccca tgctcaatac cagtcggttc aggccatgtt gatggaacca
    47581 ggcagcaact tttgccacat ctccacgccc tgacagcgca atcccactca gtgtttccgc
    47641 ttcaaggcgg tttggcttca gagtgtggat ctgatttaga cggtcgcgca ctttgacaca
    47701 tttccacgct gaaaccggat cgacaaatac gggtacgttg gcagcattat ccagaatcca
    47761 tgccagtgcc tcttcactga tattacagtc cgcgacaatg acctttgccc tctgaataaa
    47821 ttcacggtgc tgtgcgagat attcagctgt aatagcgttg ctaatattca tgtcatttat
    47881 agcaaccagc atttcaccgg tattatcgag taatgataaa taactcgacg tattttctcc
    47941 cggcacaatc aggcatttat cgacataaac gccagattga ttggtttgcg ttagcagcga
    48001 ttgaccataa aaatcactac ctactgcgct cagtaaccag gctttgttac ccagcaacgc
    48061 caggttttgt gcaatatttc gccctactcc accaggtgta aattttattt tacctggatt
    48121 tgaatccgca taatttaatg attcatgtga atatccggcg acatcaatat tcgccgaacc
    48181 tataattacg acataatcct tttcgcgcat agcacgtcct ctggtaaaaa attaccaacc
    48241 ctgtcaatcc acaagaatat agatactgca aagacagttt aaacatgtgt tcatatttaa
    48301 acatgtgctc atagtatgta tgttttacgt aaagtaaatt gccgccagag aggcaaagcg
    48361 ggcagatttg agcaggctca caaaatgcga ttaaatgcca gaattacaaa agcgaggaat
    48421 ttgtgacggg atgcacagaa caggaacact aaaaaagggc agaaaagaat ctgcccttga
    48481 ggaaataacc ttagtgtttg atgaacgttt caggtttcta ctgaaagata aaaccattta
    48541 aattcataaa attacattca caaaccggtc attctctttg cgctcatcca atgatacagg
    48601 gcagtaaatt tctgccctgt aacacacctt ttattacgcg gctttcgcta ctgcgtccac
    48661 ttccggacgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc
    48721 taccaggtaa cccagtaccg gcgtaatagc gccagggatc agcagaacaa acagaccacc
    48781 gtgcggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc
    48841 aacgatacaa cacggcagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc
    48901 cgaaatgaag cacagtccca ataccagagc ggctttgcca ccttcctgct gcgctttgtc
    48961 gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc
    49021 cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc
    49081 gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc
    49141 ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca acccttcgag
    49201 aatgccagca actggtttac cgatcaggta gatcatcgcc agaccgacca ccagactgga
    49261 aattagcgga atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcagttg
    49321 cgtactgatt aacttcgcaa tgtaaccagc caggaagccc gcaataatac cgccaatgaa
    49381 gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggcgtga gacccggacg
    49441 atcggcaatg gagaaggcaa tgtaacctgc cagtaccggc accatcagcg caaaggctga
    49501 accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat
    49561 accaaaagca aaagaaagcg cgatacacag accacctgca acgaccatcg gcagcatgta
    49621 agagacgccc gtcagcaagt gacggtatgc gcctgcactc tctttcttac cttcagaggt
    49681 cgccgtttga gctttgcccg ccggttcata cggcgttgct tcagcaaccg ctttatccag
    49741 ttcctgcgcg gttttcttca gcgccagacc ggtagaggta cgatacatcg gtttaccagc
    49801 aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg ctgcggctac
    49861 ttcttcgggg gtgattgcat tacccgcacc aacagaacca cgggtttcaa ctttcaccca
    49921 ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac
    49981 gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcgg caaccggtgc
    50041 tgtcgcagcg accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag
    50101 ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc
    50161 gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc
    50221 gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc
    50281 gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct
    50341 gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg
    50401 taatacccac attgctttga cttaccgcca gggctgcaac agcagtcgcc agacgcagtg
    50461 tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc
    50521 ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg
    50581 cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt
    50641 cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc
    50701 agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta
    50761 ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca
    50821 tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca
    50881 tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcggggg
    50941 tgacttcaaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa
    51001 cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga
    51061 acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa
    51121 ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg
    51181 tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat
    51241 aagccggatt aagggtgata gtagcaacac gtctgctcat tatgcgccct ccccaagacc
    51301 agcagcgata gcgtcgccga ttgctttcag cgcctgttca gcatctgcac cctgggcggt
    51361 aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc
    51421 gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt
    51481 aatggtgttg accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac
    51541 aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa
    51601 cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaaca aatcagcgag
    51661 acgcttaaga accgcaatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc
    51721 cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag
    51781 attgccttcg gcgctatcgc tcagccagat accctgtccg aggttcagcg gttgttcatt
    51841 gatggctttg gtgacgaaag tggcgtcaac tgccctcgcc tctttcagac gcgcagcgtt
    51901 cagcgcctga agagtcagca gatcgctggc ggcgatatcc agcgtcagca tttcgttgtc
    51961 gagcttcagc tgctcactct gcttttcgcc catcagcaat gcgcgaagtt cttctgctgt
    52021 tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg
    52081 tagcaggccc agatgttcat ccgagctggc agcaataccg atcgctacgt acgctacctg
    52141 accgtcaccc caggtgacgc cttccgtgaa ctgaaatacc tgaacgccgg ttttcagcac
    52201 ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga
    52261 ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacgt taccggcctg
    52321 caccagcgcc gcagcgagct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg
    52381 atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat
    52441 tcagcttcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg
    52501 aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat
    52561 gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa
    52621 atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg
    52681 cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg
    52741 taaactccgc gtcttcctct tccagtgatc gaccagcatg cataactccc ccgcagtctc
    52801 cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg
    52861 tattgcgggc gctctgcaaa ccccgacact cagtattttt cttaccgatg aagtacatgc
    52921 ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag
    52981 tcagtttctc gccgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg
    53041 ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt
    53101 gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc
    53161 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
    53221 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
    53281 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
    53341 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    53401 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattaat
    53461 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    53521 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    53581 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    53641 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct
    53701 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    53761 ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac
    53821 ttcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg agatctggaa
    53881 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    53941 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    54001 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    54061 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    54121 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    54181 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    54241 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc
    54301 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    54361 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    54421 gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    54481 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    54541 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    54601 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    54661 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    54721 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    54781 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    54841 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    54901 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    54961 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    55021 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    55081 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    55141 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    55201 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    55261 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    55321 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    55381 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    55441 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    55501 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    55561 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    55621 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    55681 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggtcatgt
    55741 ggtgaaaaac gcggtgctgg gaatggcaga aaaacgttcg ccagaactcg ccgggtggat
    55801 aaaagagcac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    55861 cgaatcactg gtggaaatca gccagcatct gggggtgaat gatccctgcg cgattagctg
    55921 cgaaccgttt atccagtggg tggtggaaga taacttcgtc gctgggcgtc ctgcctggga
    55981 agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcggat
    56041 gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat tcgcccatat
    56101 cagtgattgc atgcaggatc gcgcatttcg ccatgccgcc agaacattaa tgctggatga
    56161 gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat
    56221 tgcacgtttt gctaatccgg cgctgaaaca taagacctgg caaatcgcga tggatggcag
    56281 ccagaaatta ccgcaacgca tgctggcagg tattcgcata catcaggggc gcgaaacgga
    56341 ctggtcgttg ctggcattag gcgttgcagg ctggatgcgt tacgtcagcg gcgttgatga
    56401 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    56461 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    56521 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    56581 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    56641 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgctt tttttagcca
    56701 ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    56761 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    56821 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    56881 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    56941 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    57001 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    57061 cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc tcgatccgcg
    57121 cttgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    57181 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    57241 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    57301 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    57361 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    57421 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    57481 cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    57541 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    57601 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    57661 cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac
    57721 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    57781 ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt
    57841 cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag
    57901 aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc
    57961 gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac
    58021 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    58081 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    58141 accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat
    58201 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    58261 ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga
    58321 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    58381 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    58441 aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    58501 atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc
    58561 gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac
    58621 tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    58681 taaaaccgct
//
LOCUS       DAZFDP010000010        58530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFDP010000010 DAZFDP010000000
VERSION     DAZFDP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHJ1G_000720"
     CDS             complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHJ1G_000720"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4762623.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLAVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVL"
     gene            complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHJ1G_000721"
     CDS             complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHJ1G_000721"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4762624.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHJ1G_000722"
     CDS             complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHJ1G_000722"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4762625.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHJ1G_000723"
     CDS             complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHJ1G_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4762626.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHJ1G_000724"
     CDS             complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHJ1G_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4762627.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHJ1G_000725"
     CDS             complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHJ1G_000725"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4762628.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHJ1G_000726"
     CDS             4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHJ1G_000726"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4762629.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLSARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7380..9914
                     /gene="mrcB"
                     /locus_tag="ACHJ1G_000727"
     CDS             7380..9914
                     /gene="mrcB"
                     /locus_tag="ACHJ1G_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4762630.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10134..12377
                     /gene="fhuA"
                     /locus_tag="ACHJ1G_000728"
     CDS             10134..12377
                     /gene="fhuA"
                     /locus_tag="ACHJ1G_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4762631.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TVAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12428..13225
                     /gene="fhuC"
                     /locus_tag="ACHJ1G_000729"
     CDS             12428..13225
                     /gene="fhuC"
                     /locus_tag="ACHJ1G_000729"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4762632.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13225..14115
                     /gene="fhuD"
                     /locus_tag="ACHJ1G_000730"
     CDS             13225..14115
                     /gene="fhuD"
                     /locus_tag="ACHJ1G_000730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4762633.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14112..16094
                     /gene="fhuB"
                     /locus_tag="ACHJ1G_000731"
     CDS             14112..16094
                     /gene="fhuB"
                     /locus_tag="ACHJ1G_000731"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4762634.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16129..17409)
                     /gene="hemL"
                     /locus_tag="ACHJ1G_000732"
     CDS             complement(16129..17409)
                     /gene="hemL"
                     /locus_tag="ACHJ1G_000732"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4762635.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17575..17622
                     /gene="yadX"
                     /locus_tag="ACHJ1G_000733"
     CDS             17575..17622
                     /gene="yadX"
                     /locus_tag="ACHJ1G_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4762636.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17634..19055
                     /gene="clcA"
                     /locus_tag="ACHJ1G_000734"
     CDS             17634..19055
                     /gene="clcA"
                     /locus_tag="ACHJ1G_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4762637.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19079..19144
                     /gene="yadW"
                     /locus_tag="ACHJ1G_000735"
     CDS             19079..19144
                     /gene="yadW"
                     /locus_tag="ACHJ1G_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4762638.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19137..19481
                     /gene="erpA"
                     /locus_tag="ACHJ1G_000736"
     CDS             19137..19481
                     /gene="erpA"
                     /locus_tag="ACHJ1G_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4762639.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19528..20151)
                     /gene="yadS"
                     /locus_tag="ACHJ1G_000737"
     CDS             complement(19528..20151)
                     /gene="yadS"
                     /locus_tag="ACHJ1G_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4762640.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20189..20989)
                     /gene="btuF"
                     /locus_tag="ACHJ1G_000738"
     CDS             complement(20189..20989)
                     /gene="btuF"
                     /locus_tag="ACHJ1G_000738"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4762641.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20982..21680)
                     /gene="mtnN"
                     /locus_tag="ACHJ1G_000739"
     CDS             complement(20982..21680)
                     /gene="mtnN"
                     /locus_tag="ACHJ1G_000739"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4762642.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21764..23281
                     /gene="dgt"
                     /locus_tag="ACHJ1G_000740"
     CDS             21764..23281
                     /gene="dgt"
                     /locus_tag="ACHJ1G_000740"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4762643.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23411..24835
                     /gene="degP"
                     /locus_tag="ACHJ1G_000741"
     CDS             23411..24835
                     /gene="degP"
                     /locus_tag="ACHJ1G_000741"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4762644.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            24992..26143
                     /gene="cdaR"
                     /locus_tag="ACHJ1G_000742"
                     /pseudo
     CDS             24992..26143
                     /gene="cdaR"
                     /locus_tag="ACHJ1G_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26232..26618)
                     /gene="yaeH"
                     /locus_tag="ACHJ1G_000743"
     CDS             complement(26232..26618)
                     /gene="yaeH"
                     /locus_tag="ACHJ1G_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4762645.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26780..27592)
                     /gene="yaeI"
                     /locus_tag="ACHJ1G_000744"
     CDS             complement(26780..27592)
                     /gene="yaeI"
                     /locus_tag="ACHJ1G_000744"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO4762646.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27647..28471)
                     /gene="dapD"
                     /locus_tag="ACHJ1G_000745"
     CDS             complement(27647..28471)
                     /gene="dapD"
                     /locus_tag="ACHJ1G_000745"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4762647.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28502..31174)
                     /gene="glnD"
                     /locus_tag="ACHJ1G_000746"
     CDS             complement(28502..31174)
                     /gene="glnD"
                     /locus_tag="ACHJ1G_000746"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4762648.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31236..32030)
                     /gene="map"
                     /locus_tag="ACHJ1G_000747"
     CDS             complement(31236..32030)
                     /gene="map"
                     /locus_tag="ACHJ1G_000747"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4762649.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32398..33123
                     /gene="rpsB"
                     /locus_tag="ACHJ1G_000748"
     CDS             32398..33123
                     /gene="rpsB"
                     /locus_tag="ACHJ1G_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4762650.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33381..34232
                     /gene="tsf"
                     /locus_tag="ACHJ1G_000749"
     CDS             33381..34232
                     /gene="tsf"
                     /locus_tag="ACHJ1G_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4762651.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34379..35104
                     /gene="pyrH"
                     /locus_tag="ACHJ1G_000750"
     CDS             34379..35104
                     /gene="pyrH"
                     /locus_tag="ACHJ1G_000750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4762652.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35396..35953
                     /gene="frr"
                     /locus_tag="ACHJ1G_000751"
     CDS             35396..35953
                     /gene="frr"
                     /locus_tag="ACHJ1G_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4762653.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36045..37241
                     /gene="ispC"
                     /locus_tag="ACHJ1G_000752"
     CDS             36045..37241
                     /gene="ispC"
                     /locus_tag="ACHJ1G_000752"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4762654.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37430..38188
                     /gene="ispU"
                     /locus_tag="ACHJ1G_000753"
                     /gene_synonym="uppS"
     CDS             37430..38188
                     /gene="ispU"
                     /locus_tag="ACHJ1G_000753"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4762655.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38201..39058
                     /gene="cdsA"
                     /locus_tag="ACHJ1G_000754"
     CDS             38201..39058
                     /gene="cdsA"
                     /locus_tag="ACHJ1G_000754"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4762656.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39070..40422
                     /gene="rseP"
                     /locus_tag="ACHJ1G_000755"
     CDS             39070..40422
                     /gene="rseP"
                     /locus_tag="ACHJ1G_000755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4762657.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40452..42884
                     /gene="bamA"
                     /locus_tag="ACHJ1G_000756"
     CDS             40452..42884
                     /gene="bamA"
                     /locus_tag="ACHJ1G_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4762658.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43006..43491
                     /gene="skp"
                     /locus_tag="ACHJ1G_000757"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43006..43491
                     /gene="skp"
                     /locus_tag="ACHJ1G_000757"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4762659.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43495..44520
                     /gene="lpxD"
                     /locus_tag="ACHJ1G_000758"
     CDS             43495..44520
                     /gene="lpxD"
                     /locus_tag="ACHJ1G_000758"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4762660.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44625..45080
                     /gene="fabZ"
                     /locus_tag="ACHJ1G_000759"
     CDS             44625..45080
                     /gene="fabZ"
                     /locus_tag="ACHJ1G_000759"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4762661.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45084..45872
                     /gene="lpxA"
                     /locus_tag="ACHJ1G_000760"
     CDS             45084..45872
                     /gene="lpxA"
                     /locus_tag="ACHJ1G_000760"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4762662.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45872..47020
                     /gene="lpxB"
                     /locus_tag="ACHJ1G_000761"
     CDS             45872..47020
                     /gene="lpxB"
                     /locus_tag="ACHJ1G_000761"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4762663.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47017..47613
                     /gene="rnhB"
                     /locus_tag="ACHJ1G_000762"
     CDS             47017..47613
                     /gene="rnhB"
                     /locus_tag="ACHJ1G_000762"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4762664.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47650..51132
                     /gene="dnaE"
                     /locus_tag="ACHJ1G_000763"
     CDS             47650..51132
                     /gene="dnaE"
                     /locus_tag="ACHJ1G_000763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4762665.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51145..52104
                     /gene="accA"
                     /locus_tag="ACHJ1G_000764"
     CDS             51145..52104
                     /gene="accA"
                     /locus_tag="ACHJ1G_000764"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4762666.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52203..54344
                     /gene="ldcC"
                     /locus_tag="ACHJ1G_000765"
     CDS             52203..54344
                     /gene="ldcC"
                     /locus_tag="ACHJ1G_000765"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4762667.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54401..54790
                     /gene="yaeR"
                     /locus_tag="ACHJ1G_000766"
     CDS             54401..54790
                     /gene="yaeR"
                     /locus_tag="ACHJ1G_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4762668.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54855..56153
                     /gene="tilS"
                     /locus_tag="ACHJ1G_000767"
     CDS             54855..56153
                     /gene="tilS"
                     /locus_tag="ACHJ1G_000767"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4762669.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56202..56462)
                     /gene="rof"
                     /locus_tag="ACHJ1G_000768"
     CDS             complement(56202..56462)
                     /gene="rof"
                     /locus_tag="ACHJ1G_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4762670.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56449..56649)
                     /gene="yaeP"
                     /locus_tag="ACHJ1G_000769"
     CDS             complement(56449..56649)
                     /gene="yaeP"
                     /locus_tag="ACHJ1G_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4762671.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56815..57360
                     /gene="yaeQ"
                     /locus_tag="ACHJ1G_000770"
     CDS             56815..57360
                     /gene="yaeQ"
                     /locus_tag="ACHJ1G_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4762672.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSDFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57357..57779
                     /gene="arfB"
                     /locus_tag="ACHJ1G_000771"
     CDS             57357..57779
                     /gene="arfB"
                     /locus_tag="ACHJ1G_000771"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4762673.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57793..58503
                     /gene="nlpE"
                     /locus_tag="ACHJ1G_000772"
                     /gene_synonym="cutF"
     CDS             57793..58503
                     /gene="nlpE"
                     /locus_tag="ACHJ1G_000772"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4762674.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13736 a        14537 c        16305 g        13952 t
ORIGIN      
        1 tgtaagactt cacgcaacgt ctccccatca ggaaacgcca gctccggtgc aatctcaaac
       61 agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggc caggcgttcg
      121 gtatttatca cttcattacc aaacagcatg atatcgagat ccagcgtgcg tggtccccag
      181 cgttcagctt tacgaacacg accctgttgc agttctatgc gctgtgtatg gttgagtaac
      241 tcttccgggg cgagcgaagt ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct
      301 tgcggcccca gcggtggggt gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg
      361 atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg
      421 cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg
      481 gacgacgagt acggcgacgc ggtgacggct cttcatccaa ctcgttaagc atcccttttt
      541 gatctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgc tgcagttcag
      601 cgttacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg aacttaggat
      661 gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac tgcaactgcc
      721 agatatcgcg ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt
      781 ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg
      841 caatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac aggaacgccg
      901 ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc tgttcaatga
      961 tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg
     1021 gctggaacag atgatattca cacaacagct tataggtatc gtaaccgtag cccgcttgta
     1081 gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcaac agggtagcga
     1141 ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg gcggcaaaac
     1201 gtaccgcgcg cagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca
     1261 gacggataac gccgtccttc agatccttca taccgccaac gtaatcacgg acggtaaaat
     1321 ccgctacgct gtaatagagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga
     1381 tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc
     1441 ggtcgctgac gttaccttcg tggtgtccac ggaaggtcgc aacttcgata atctccgggc
     1501 caaacattac atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt
     1561 tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa
     1621 gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttgttgagcc
     1681 tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac
     1741 gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc
     1801 ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca
     1861 aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa
     1921 tgttgaattt acaattgccg actcgggcac ggcggtaagc cgccagtttt tgacggctga
     1981 ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa
     2041 ttgaagtgcc gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt
     2101 ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg
     2161 cactttccag ccaaaaagct ggtacagcga gatttgcctt actgttggtt caatcagatc
     2221 agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa cagccaggtt
     2281 gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc
     2341 ggcgtgaata atgccgcgca gctggtcagt aaattgcgtg accggatgct gctggcggat
     2401 acgcactgcg gcattgtctg gtccatgatg caacacccgg caatgaccgt cgtaaatacc
     2461 gccaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg
     2521 taaccaggcg agtgcttcac gataggcgtg gtgacgttgc gattgccaga gaacatcgcc
     2581 gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac
     2641 ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc gggcgcgagc
     2701 ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc cggaaggaga
     2761 gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg
     2821 gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt
     2881 ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc
     2941 cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc
     3001 cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg
     3061 cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc
     3121 tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca
     3181 tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg
     3241 cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg
     3301 gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg
     3361 ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta
     3421 acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac
     3481 cagttactta ctttactaca atgtaaccgg cagtgatttt ttaagagcca tgccttcagc
     3541 agaaatttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag
     3601 ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag
     3661 cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag
     3721 gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa
     3781 cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc
     3841 aatacggctg cgttctgcgc cgtattttac ttcgcttttc agcgagctat agcctgacag
     3901 ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt
     3961 gacgcaaata aatgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg
     4021 tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat
     4081 tgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc
     4141 taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag aaaattccat
     4201 tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt
     4261 ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcatacga ccagttaaaa
     4321 cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga
     4381 aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc
     4441 agctggatta agccgcgtgg cggctgacgc atccctaacc acaccacacg cgaacgcagc
     4501 cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa
     4561 agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag agtcagatgc
     4621 aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc gcgccagtgg
     4681 ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg ttgcggttca
     4741 gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt
     4801 aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt
     4861 accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca
     4921 acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg
     4981 gagccgcgtc gtctgtcggc gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa
     5041 aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc
     5101 cgcctggaag tagttaccga aggcgtgctg acgcggatga tccagcgtga cccggagttg
     5161 agcggtgttg gattggtgat cctcgatgag tttcatgagc gcagcttgca ggcggatttg
     5221 gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt
     5281 atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc tgccagaagc gcccgtcgtc
     5341 atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccactgcc cacgcatcag
     5401 cgttttgacg aagccgttgc ggtagccacc gctgaaatgc tgcgtcagga aagtggatca
     5461 ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actggcttcg
     5521 cgcatcggca gcgatgtatt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag
     5581 cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt
     5641 gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt
     5701 gtggcgcgtt ttgatccgcg cacggggctt acgcgactga ttactcaacg cgttagccag
     5761 gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat
     5821 ttaatcgcca aggaacaagc agaacgcgcc gcggcgcaaa gtgaaccgga gatattacaa
     5881 agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag
     5941 atgagctggc tggatcaacc gccagtagtg aatctactgg ccgcgaaacg tctgttacaa
     6001 atgctggggg cactggaggg tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg
     6061 ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga cgaagctgct
     6121 accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg
     6181 ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca actgttaaaa
     6241 cgcttaaacg tacgtggcgg tgaggcagac agttcgctta tcgcgccgct acttgccggg
     6301 gcgtttgccg atcgcattgc tcgtcgccgt gggcaagatg gacgctatca actggcaaac
     6361 ggcatgggag cgatgctcga tgccaacgac gcgctaagcc gccacgaatg gttgatcgca
     6421 ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctgccggtc
     6481 gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga cactgtggag
     6541 tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg tctgctgacg
     6601 gtgaaagtgc agccgctggc gaaaccctcg gaagacgagt tgcatcaggc gatgcttaat
     6661 ggcatccgcg ataaagattt aagcgtgctc aactggacgg cggaagcgga acagctacgc
     6721 ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc ggttgatgat
     6781 gaaagtttat tggcgacgct ggaaacgtgg ctgctgccac atatgactgg cgtacattca
     6841 ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact tgattgggga
     6901 atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg
     6961 atcgccattc gttatcatga agataacccg cccgcgctgg cggtgagaat gcaagagatg
     7021 tttggcgagg ccaccaatcc gacgatcgcc caggggcgcg tgccgctggt gctggagttg
     7081 ctttcacctg cccaaaggcc attacaaatc acacgagatt tgagcgcctt ctggaaagga
     7141 gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt ctggccggac
     7201 gacccggcaa atactgcacc gacgcgacgg acgaaaaagt attcgtaagg ttggttttct
     7261 ccctctccct gtgggagagg ggaccgatcg agcacaaatt ttgagagata tcttcttctg
     7321 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
     7381 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac
     7441 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
     7501 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg
     7561 gcaaacgcgg ctggctatgg ctgctgttaa aactggctat cgtttttgcc gtgctgatcg
     7621 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
     7681 aactgcctgc ggcagtttat ggccgaatgg tcaaccttga gccagatatg accatcagca
     7741 agaacgagat ggtgaagctg ctggaggcga ctcagtatcg tcaggtgtcg aaaatgaccc
     7801 gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt
     7861 tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggc gatcatctgg
     7921 cgacgatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgtc
     7981 tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
     8041 tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt tacgagcatg
     8101 acggcatcag cctctactcc atcggacgtg cggtgctggc aaacctgacc gccggacgca
     8161 cggtgcaggg agcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagtg
     8221 agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt
     8281 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     8341 acaacgaaat ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccggtagaag
     8401 agctaagcct cgaccagcag gcgctgttag tcggcatggt gaaaggagcg tccatctaca
     8461 acccgtggcg taacccaaaa ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc
     8521 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcacgt ccgctgggtg
     8581 ttcagccacg cggtggggtg atctctccac agccagcctt tatgcaactg gtgcgtcagg
     8641 agctgcaggc gaaattaggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     8701 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac
     8761 tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta
     8821 gtggtgaagt tcgtgcgatg gtcggtggtt ctgagccgca gtttgcgggc tacaaccgtg
     8881 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgactgcct
     8941 taagccagcc gaaaatctat cgtctgaata cgtggattgc ggatgcgcca atcgcgctgc
     9001 gtcagccgaa tggccaggtc tggtcaccgc agaatgatga ccgtcgttat agcgaaagcg
     9061 gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg
     9121 ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc gtaccgaaag
     9181 atcagttgca tccggttccg gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     9241 tggcgcaggc gttccagacc atcgccagcg gcggtaaccg tgcaccgctt tctgcactgc
     9301 gttcggtaat tgcggaagat ggcaaggtgc tgtatcagag cttcccgcag gcggaacgcg
     9361 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgcg
     9421 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     9481 ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg gtgaccatca
     9541 cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt
     9601 cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt gttccgccag
     9661 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     9721 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc gagatgcagc
     9781 agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc
     9841 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc aaggatatgt
     9901 ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat ccggtaaaat
     9961 aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta acccttcagg
    10021 aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt
    10081 ataataatca ttctcgttta cgttatcatt cactttacat cagagatata ccaatggcgc
    10141 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
    10201 cggtaagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
    10261 ccgttaccgt tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
    10321 cgcgacagtc tgctaccggc actaaaaccg atacgccgat tcaaaaagtg ccacagtcta
    10381 tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc
    10441 ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc tatgaccacc
    10501 tgatcattcg cggctttgcg gcagaaggcc aaagccagaa taactatctg aatggcctga
    10561 agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg gaacgcgctg
    10621 aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc ggcctgttga
    10681 atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt aaagccggta
    10741 ctgacagcct gttccagact ggttttgact ttagcgattc gttggatgat gacggtgttt
    10801 actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag
    10861 agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa accaatttca
    10921 ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg ttgccgaaag
    10981 agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt aatgaagggg
    11041 cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc gatcacgaat
    11101 ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa
    11161 acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc aaacagtgtg
    11221 cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc gttgatgatg
    11281 agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc actggcgata
    11341 tcgaccacac cctgctgtcc ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct
    11401 ggtttggtta cgacgactct gtgccactgc tcaatctgta caatccggtg aataccgatt
    11461 tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg aataaacaga
    11521 aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg gtcaccctag
    11581 gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg acgaccgata
    11641 aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt gataatggtg
    11701 taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg
    11761 gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg
    11821 aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc aacaacctga
    11881 tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg
    11941 tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt
    12001 acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct gcacaggtgc
    12061 caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg ctttcaggtc
    12121 tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg gctaactcct
    12181 ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg
    12241 gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg
    12301 ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
    12361 caaccttccg tttctaattt ctcttttggg gcacggattt ccgtgcccat ttcacaagtt
    12421 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
    12481 ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
    12541 agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca aaatgcttgg
    12601 ccgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc tggaaagctg
    12661 gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc ctccggcaga
    12721 agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg
    12781 gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa
    12841 accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc gggcgtggat
    12901 cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga cttcggcgct
    12961 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggaacgtgg
    13021 cctgacggtc attgccgtgc tgcacgatat caatatggcg gcacgctact gtgattatct
    13081 ggtcgccctg cgcggtggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
    13141 cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc cggcgggtgc
    13201 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
    13261 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
    13321 attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact gctggcgctc
    13381 ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt cagcgaacca
    13441 ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct tgaactgctg
    13501 accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc accagaaatg
    13561 ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca gccgttggcg
    13621 atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag cgcagcagaa
    13681 acgcatttag cgcactatga agactttatc cgcagcatga aaccccgctt tgtgaagcgt
    13741 ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
    13801 ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc ctggcaaggg
    13861 gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcggc gtataaagac
    13921 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacc
    13981 ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt acccgcagtc
    14041 tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga taacgccatc
    14101 ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg gcgctgttag
    14161 tgattgtcgc tacggcgcta acctggatga acttctcaca ggcgctgccg cgcagtcagt
    14221 gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt tttcactaca
    14281 gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg
    14341 tgctgtttca gcaagtgctg cgtaatccgc tggcggaacc gacgacactt ggcgttgcta
    14401 caggcgcgca actgggaatt accgtcacca cactttgggc gatcccaggt gctatggcga
    14461 gccagtttgc cgcactggtg ggcgcttgtg tcgttggctt aatcgtcttt ggcgtcgcgt
    14521 gggggaaacg gctttcgccg gttacgctta ttctcgcggg gctggtggtg agcctttatt
    14581 gcggggcaat taatcagcta ctggttatct tccatcacga ccagctgcaa agcatgttcc
    14641 tgtggagcac cggaacgctg acgcaaaccg actggggcgg cgttgagcgt ttatggccgc
    14701 agttgctggg cggcgtgatg ttgacgttat tgctacttcg cccgttaacc ctgatggggc
    14761 ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gctcgcgcgt ctggcagcgc
    14821 tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt atcggcttta
    14881 tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg ctgccacgac
    14941 tgatgctggc gtcgctgatt ggggcgctga tcctctggct ttccgatcaa atcatcctct
    15001 ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc
    15061 cgctgctact gtggctgttg ccgcgtttac gcagcattag cgcgccggat atgaaggtca
    15121 acgatcgtgt cgcggctgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
    15181 tgctgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
    15241 gggcgagcgg ggcgttgctt gaggatttga tgccctggcg ctggccgcga attatggcgg
    15301 cgctgtttgc gggcgtcatg ctggcggtgg cgggctgtat tattcagcgg ctgaccggaa
    15361 acccgatggc aagcccggaa gtgctgggga ttagctctgg cgcagcgttt ggcgtggtgt
    15421 tgatgctgtt tctggtgccg ggtaatgcct ttggctggct gttacctgca ggcagtctcg
    15481 gcgcggcggt gacgctgttg atcattatga tcgccgccgg ccgcggtgga ttttccccac
    15541 accgcatgtt actggcgggg atggcgttaa gcaccgcgtt caccatgctt ttgatgatgt
    15601 tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc ggttcgacct
    15661 acaacgcgac cgatgcgcag gtctggcgca cgggaattgt gatggtgatt ttgctggcga
    15721 ttaccccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcccgag
    15781 ccgtaggaat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
    15841 cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg gcaccgcata
    15901 ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg gcgctggtgg
    15961 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccattccaga
    16021 tcccagcagg gctgctgtcg acctttatcg gcgcgccata ttttatctat ttgttgagaa
    16081 agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaatcatc acaacttcgc
    16141 aaacacccgt cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacgga
    16201 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg
    16261 gaagaaacgc ttaaagcgtt ccacgtcaca ggccatcaca tcctgatagc acgtcacgga
    16321 ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggttaacga ccagcggaat
    16381 tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
    16441 cgtttcgtga acgcccggct gcgcgacttc attcagacag gcgaaacccg ctgccatcgc
    16501 aattgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg ccagcgcatc
    16561 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgattttgcc
    16621 caggcaggtg agatccggtt ccacaccgta ataatcctgt gcgccagcta gcgctacgcg
    16681 gaagccagtc attacttcat cgatgatcag caatgcgcca aattcgtcgc acagcgcgcg
    16741 cagacctggc aggaactctg gcagcggtgg aacgcagttc atattgcctg ccaccggctc
    16801 gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaagccag
    16861 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacac ccggcgagtt
    16921 tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt cagcgtgacc
    16981 gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg
    17041 gatggcgctc atcgtcgctt cagtgccgga gttcaccatg cgcaccatat ccatggtcgg
    17101 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg caccaaagct
    17161 taaaccacgc tcggcggctt caatcacggc attgcggata gccggatggt tatggcccag
    17221 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    17281 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    17341 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    17401 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ctatgttatt
    17461 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    17521 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    17581 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    17641 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    17701 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    17761 gcacgcttgt cgggctggca gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    17821 aacgtatggg ggcgctggta catactgctg ataattatcc gcttctgtta accgtcgctt
    17881 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg
    17941 aagcaggtgg ttcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc
    18001 gctggtggcg tgtgttgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    18061 tggtgctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcggt cgcatggtgc
    18121 ttgatatttt ccgcctgaaa ggtgatggag ctcgccatac gctgctggca accggtgctg
    18181 ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    18241 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggcgtca
    18301 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    18361 aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    18421 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    18481 tgcacggcgg caatattacc aaatgggtac tgatgggtgg tgcgattggc ggtctgtgtg
    18541 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    18601 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat tttcgtcgct cgggtcatta
    18661 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    18721 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    18781 acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg gcatctattc
    18841 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    18901 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    18961 cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctatg caactggcgc
    19021 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    19081 ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag caaaatatga
    19141 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    19201 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    19261 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    19321 tcgaaaaaca gggcgttggc ttggtggttg atccgatgag cctgcaatat ctggtcggcg
    19381 gttccgttga ctataccgaa ggactggaag gttctcgttt catcgtgacc aacccgaacg
    19441 cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt catgtgccgg
    19501 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    19561 tagcttaaga tgccaacgaa tagccgccag ccgaatcaag agcgtcacga ccatgcccat
    19621 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    19681 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc
    19741 cagaacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    19801 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
    19861 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
    19921 cacgatggtc agcatgctgg ttaccattgc aacgaccaga tcggtgggat ctttcaccca
    19981 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac
    20041 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    20101 ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    20161 cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca ttacagagct
    20221 gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    20281 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    20341 cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    20401 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    20461 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    20521 gttttttagg tttatcagca tattgcgctt tcaattgcgc gtactgatcc agcagggatt
    20581 gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga cgtaacgcat
    20641 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    20701 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    20761 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    20821 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    20881 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    20941 gcgcaagaaa agacagggcg accagcgccc tgaacagtga cttagccatg tgcaagtttc
    21001 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    21061 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    21121 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    21181 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    21241 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    21301 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    21361 taaccaaatg ccgtgacatc cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg
    21421 cccactttca acgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    21481 ttgcagtgtt ccaacagcaa agtggcaccc agcgccgcag cgactttacc gatgcccgat
    21541 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    21601 ctgatagttt gacgtttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    21661 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    21721 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    21781 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    21841 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    21901 tcgtctgcaa caaaagacgc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    21961 tcttacccac tcgatggaag tccagcaggt ggggcgctac atcgccaaag aaattttaag
    22021 ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga ccggaccttt
    22081 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    22141 ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg aagatgccga
    22201 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    22261 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    22321 tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcacct gggcacaggt
    22381 tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca
    22441 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccggtt
    22501 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacttgga ttatggaagc
    22561 tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga aaagaatatt
    22621 taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg agaaaggttc
    22681 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    22741 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    22801 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    22861 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata aaaatgtcgc
    22921 tgtaaaacat gtgtttagcc atccagatgt cgagcagctt gaattgcagg gctatcgggt
    22981 cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat cagactttac
    23041 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gaatcgcgct tattccacaa
    23101 actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac cgtcagattc
    23161 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    23221 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    23281 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    23341 acttcgcgct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    23401 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    23461 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    23521 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    23581 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    23641 tgatgattct ccgttctgcc aggaaggttc accgttccag agctctccgt tctgccaggg
    23701 gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg gttccggcgt
    23761 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    23821 ggtgattaaa gtccagctgg gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga
    23881 tccgcgctct gatatcgcgc tgattcagat ccagaacccg aaaaacctga ccgcaattaa
    23941 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    24001 tggcctggga gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    24061 tgccgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    24121 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    24181 ggacggcggc aacatcggta tcggttttgc tatcccgagc aacatggtga aaaacctgac
    24241 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    24301 gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    24361 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    24421 ctcactgaac ggtaagccaa tcagcagctt tgccgcactg cgtgctcagg tgggtactat
    24481 gccggtgggc agcaaactga ccctgggctt gctgcgcgac ggtaagcagg tcaacgtgaa
    24541 tctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    24601 tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    24661 aacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa
    24721 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt cttgacagca aaccgtctgt
    24781 actggcactg aacattcagc gcggcgacag ctccatctac ctgttaatgc agtaatctcc
    24841 ctcaacccct tcctgaaaac gggaaggggt tctccttaca atctgtgaac ttcaccacaa
    24901 ctccatacat cttcatcatc ctttaggcat ttgcacaatg ccgtacgtta cgtacttcct
    24961 tatgctaagc cgtgcataac ggaggactta tggctggcat cttgatacca aaatggcgca
    25021 ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg
    25081 tgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt
    25141 gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtggcac gtcatctgca
    25201 cggtgtgcgg caagggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat
    25261 tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc
    25321 tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gctcaggata gccgtttgcg
    25381 ggaggaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac ttactgaatg
    25441 ggcgcaacgg ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga
    25501 cagcggtcag cttggtgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct
    25561 gactacgccc gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt
    25621 gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga
    25681 acaactgatt acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa
    25741 ctattttacc ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt
    25801 ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc
    25861 tgtgttactc gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc
    25921 gcggctgaaa gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg
    25981 tcacaatgtg caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga
    26041 gtatcggctt aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag
    26101 gttgctgctg tatgtggcgt tacaactgga tgaagagcgg taggttatgc gttaaggttg
    26161 aggcggcgct ggctcattcc cctcagcccg aaggggagag gggaatgccc gaattaaccg
    26221 tcaattcggg attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta
    26281 ttggttacga ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact
    26341 tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg tagattcggg
    26401 ctgatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc
    26461 tgacgcggat atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa
    26521 tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca
    26581 ggattggtaa tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca
    26641 gtaaattgtg cttattttag catttggcct ggcccgcccg gctcgactgt gatcgcagaa
    26701 agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg
    26761 tggtgataaa attcacgttc tacaccagtt ccagcatcgt tacttccggg cggcagttca
    26821 gacgcaaacc atacaaactg cccacgccac gggttgtgta gatgtgtcgt tcgccaaagg
    26881 catttaatcc ggcgacgtaa cgtttatctt cgacaggggc aaaaggttca ccaaccaacg
    26941 gtacgcgcag ttgcccgccg tgagtatggc cgcacagcat cagatcccag ggttcatcac
    27001 gcatgacttc tttgctgtcg ggattatgcg ccagcaccag tcttggcaga ttggcttcgc
    27061 tggcgggagg cggtttgcat tgtccggccc ataagtcacc ggtgcctacc agttcgaatt
    27121 gcctgttcgg cgtggcgatc accgtagcct ggttaaacaa caccgtgatg cccgctgatt
    27181 tcaacgtctc gccaattaag tgattttttt ctgtaccaac agggcgatcg tggttgccga
    27241 agcaggcaaa cgtcggcgca cattcggcaa ggggggagag gacatcacta aacgccgaaa
    27301 aattcagcgg catatcaaat aatacgtaat cgccgcccag taatatcaaa tcgggctttt
    27361 gttctatgcc aagagcaatc gcgtcagaaa tcaggcttaa aggaacaaaa cgagagtaat
    27421 ggagatcggc cagaaaaaga attttgaatg gtgctgcgtt gtctttaaaa aaggcgaggc
    27481 ggtgacggat taattcaaac cagcctggct cccagtaatg catataaccg aaacctgagc
    27541 tcgtggctat cgtcgcggca gtagcctgca acaatcggcg gcgtgaaatc atcgctcatc
    27601 cctgcaaaaa aaatcgggca gcgtcgtgct gcccgtgtgc atacttttag tcgatggtac
    27661 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27721 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27781 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27841 tctgaccaat gtatacgccc atggaaatga cggaaccttc ttcgacaatc accccttcaa
    27901 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat gatggttggg ttagcctgca
    27961 gcggttccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    28021 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    28081 cgatgttgac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    28141 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    28201 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    28261 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    28321 cgtcaatttt ttccgctaca cgcagtgcac cggaatccag cagggcgatc acctgattta
    28381 ccgcttcgcg ggtaacggtg tctgcgttgg ctggcgtgat ctcggcacgg cgttcaaaag
    28441 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28501 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28561 actcgttatt aagtgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28621 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28681 cgactctcgc cagcagtcct ggctggtcga gcgcgataag ctcaaggaac gatttgcggt
    28741 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28801 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagtacttgc tccagaccaa
    28861 accgaataac ctcatgacga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28921 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28981 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    29041 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    29101 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    29161 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    29221 tgcgcagtag tgccagtgcc tggagttggt gatgacgaac ccgttcgcgc atatccggcg
    29281 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    29341 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc accgtcaggc
    29401 ataccagata acgaagtcgg ttttctgttt gcacttcttc ggcaaactgc ttgatgactt
    29461 ccgggtcctg aatatcgcgg cgttgggcgg tcaccgacat caacaggtgc tggcgaacca
    29521 gccaggcaac cagctgtgtt tcgcgcgagt taagcccgtg gagttcggca aaatgcacta
    29581 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29641 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29701 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29761 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29821 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29881 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttcc ggaatattac
    29941 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    30001 tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    30061 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    30121 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    30181 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    30241 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    30301 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    30361 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    30421 caaacccaac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30481 gagtgtggat atcgcgcaag ccgccagggc tacttttgat gtctggttca agattgtagc
    30541 tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc gcgtagaact
    30601 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30661 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30721 cttccagcat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30781 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30841 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30901 cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30961 ggagctggtc gataaactcg gtgcgcgcct caatcaactg ttctgcagag atcccattgt
    31021 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccaccgacgg
    31081 ttaattcatc acggggccag acgcatggat tttgcggttg accgggcagg gtggggagag
    31141 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    31201 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    31261 gggatggtgt catccttgcg tagcgtcaga atttcgcagc cgttatcagt caccacaata
    31321 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    31381 atggtgcgga tctctttttt acccgcgttg accattggct cgatggtgaa cgtcatccca
    31441 ggtttcagta cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31501 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31561 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt gaccatgcgc
    31621 agcgccaggt acaggctttc ttgcgtgatg cggcacagac gttcgcccat gatggtcggc
    31681 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31741 tcaatgttaa cgatatcgcc atctttcagc agcttcgcat cgtccgggat accgtggcac
    31801 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31861 gaaaccgtgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31921 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31981 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    32041 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    32101 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaacgtttt ctataataga
    32161 aaattcaacg tctgatgctg tacacagcgc caacaattat tggtgtccat gacgtatttg
    32221 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    32281 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    32341 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    32401 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    32461 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32521 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgct
    32581 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32641 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32701 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32761 caggacggta ctttcgaaaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32821 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32881 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cctgggtatt
    32941 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    33001 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    33061 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    33121 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    33181 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    33241 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    33301 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    33361 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    33421 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33481 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33541 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33601 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33661 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33721 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgagaacatc aacattcgcc
    33781 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33841 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33901 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33961 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    34021 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    34081 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    34141 ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    34201 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    34261 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    34321 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    34381 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    34441 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34501 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34561 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34621 gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34681 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34741 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tctgccggta caggtaaccc
    34801 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34861 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34921 aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34981 cctagcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    35041 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    35101 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgttag
    35161 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    35221 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    35281 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    35341 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    35401 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    35461 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35521 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35581 ccgtacactg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35641 tatggcgtcc gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35701 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35761 acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35821 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35881 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35941 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    36001 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    36061 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    36121 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    36181 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    36241 aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa caagccgctt
    36301 gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt ggcgctgctg
    36361 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    36421 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36481 tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa cctatccagc
    36541 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36601 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg ccggatcaag
    36661 cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg gctaccatga
    36721 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36781 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36841 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36901 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36961 catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg gaggcgttcg
    37021 aacaaggtca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    37081 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc gtactggaaa
    37141 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcgc
    37201 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    37261 agtcgcgcta tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag
    37321 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    37381 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    37441 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37501 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37561 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt
    37621 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37681 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37741 gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag aacgtattcg
    37801 taaatctgaa gcgctaacag ccgggaatag cggtctgacg ctgaatattg cggcgaacta
    37861 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37921 aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct gtatgcatga
    37981 actggcccct gtagatttag taattaggac tgggggggag catcgcatta gtaacttttt
    38041 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    38101 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    38161 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    38221 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    38281 gttcgccatc gtaacgctgg tggtctgcat gctggcagcg tgggaatggg ggcagcttag
    38341 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    38401 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    38461 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38521 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38581 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactacg acgagaatca
    38641 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactctgg
    38701 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38761 taaaacctgg caaggcttta taggtggact agcgaccgca gcggtaatct catggggtta
    38821 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38881 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38941 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    39001 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    39061 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    39121 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    39181 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    39241 gaatatgtta tcgccctgat cccgctgggc ggttatgtca aaatgctgga tgagcgcgca
    39301 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    39361 gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    39421 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggtgaaat agcagccaat
    39481 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39541 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39601 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39661 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39721 cgtgggccgc aaattgaacc tgtactggaa aatgtgcaac caaactcggc ggcaagcaag
    39781 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39841 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39901 aggcagggaa gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39961 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    40021 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    40081 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    40141 ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    40201 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    40261 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    40321 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    40381 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    40441 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40501 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40561 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg gttaatgatg
    40621 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40681 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40741 ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt
    40801 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40861 tggaagactt ctactacagc gtcggtaaat atagcgccag cgtaaaagct gtcgtgaccc
    40921 cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa
    40981 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    41041 tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa taccagaaac
    41101 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggatcgc ggttatgccc
    41161 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    41221 cggtgaacat taccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    41281 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    41341 acggcaccaa agtgaccaag atggaagatg acatcaaaaa gcttctcggt cgctatggtt
    41401 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    41461 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41521 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggc
    41581 tgggaagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41641 ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaggtagat gtcgtctaca
    41701 aggtaaaaga gcgcaacacc ggtagcttca acttcgggat tggttacggt actgaaagtg
    41761 gcgtgagctt ccaggctggt gtgcagcagg ataactggtt aggtacaggt tatgctgttg
    41821 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41881 acttcactgt agatggtgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41941 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    42001 tcccgatcaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    42061 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    42121 gcacttctga tcaggataat agcttcaaaa cggacgactt cacgttcaac tatggttgga
    42181 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    42241 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    42301 cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc cgctggggtt
    42361 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    42421 ccagcaccgt gcgtggcttc cagtccaata caattggtcc gaaagcagtt tacttcccgc
    42481 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42541 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42601 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcct
    42661 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42721 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42781 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42841 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42901 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42961 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    43021 ttagcggcag gtctcggttt agcactggca acttctgctc aggcggctga caaaattgca
    43081 atcgtcaaca tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    43141 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    43201 gctaaaatga aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa gctggaaaaa
    43261 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    43321 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    43381 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac
    43441 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43501 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43561 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43621 gttaacccaa aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc
    43681 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43741 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43801 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43861 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43921 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43981 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    44041 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    44101 attggcgatc gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    44161 actattattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    44221 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    44281 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    44341 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    44401 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    44461 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44521 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44581 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44641 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44701 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44761 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44821 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44881 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44941 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    45001 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    45061 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    45121 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    45181 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    45241 atactaaaat tggccgcgat aatgagattt atcagttcgc ctccatcggc gaagttaacc
    45301 aggatctgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    45361 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    45421 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45481 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45541 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcacgtg atggttggcg
    45601 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45661 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45721 ccgctatccg caatgcgtat aagctgattt atcgtagcgg taaaacgctc gatgaagtga
    45781 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45841 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat taacgattgc
    45901 cctggtcgcc ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45961 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    46021 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    46081 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    46141 gccagatatt tttgttggta ttgatgcgcc tgacttcaat attactcttg aaggtaacct
    46201 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    46261 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    46321 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    46381 tgccatgccg ttagatccag ataaaaatgc cgcccgtgat gtgctgggga tccctcacga
    46441 tgcccactgc ctggcgttgc taccagggag ccgtggtgca gaagttgaaa tgcttagtgc
    46501 cgatttcctg aaaacggccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46561 gccactggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46621 agacctttca gttcatttgc tggatgggat gggccgtgag gcgatggtcg ccagcgatgc
    46681 ggcgctactg gcgtcgggta cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46741 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46801 ttatgtctcg ctgccaaatc tgctggcggg tagagagtta gtcaaagaat tattgcagga
    46861 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46921 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46981 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    47041 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    47101 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    47161 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    47221 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    47281 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    47341 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg cgatagccgc
    47401 gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg
    47461 gcgctggata ttgttttccc gcaatatggt tttgcccaac acaaagggta cccaaccgct
    47521 tttcatctgg aaaaactggc tgaacacggc gcgaccgaac accatcggcg cagctttggg
    47581 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47641 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47701 atcgatggcc tggccaaaac cgcaccgttg gtaaaaaagg cggcggcgtt gggtatgcca
    47761 gcactggcga tcaccgattt caccaacctt tgtggtctgg tgaagttcta cggagcggga
    47821 catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg cgacctgctg
    47881 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47941 acgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    48001 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg acgcatgggc
    48061 gacgtcggac gcagtctttt gcgtggtaac agagcgctgg tagatgagtg tgtcgcgttt
    48121 tatgaagaac acttcccgga tcgctatttt ctcgagctga tccgcaccgg caggccggat
    48181 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    48241 gcgaccaacg acgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    48301 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    48361 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc cgaagccctt
    48421 gccaacaccg ttgagatcgc caaacgctgt aacgtaaccg tgcgtcttgg tgaatacttc
    48481 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48541 gagggcctgg aagagcgtct ggccttttta ttccctgacg aggaagaacg tgttaagcgc
    48601 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc
    48661 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48721 gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48781 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48841 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgagcac
    48901 gtagcggaca tgtacggtcg tgatgcggta tcgcagatca tcaccttcgg tacaatggcg
    48961 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgat
    49021 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    49081 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    49141 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    49201 gcgccgacca aaattaccga ttttgcgccg ctttactgcg atgaagaggg caaacatccg
    49261 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    49321 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    49381 aagaatggcg agccgccgct ggatatcgct gcgatcccgc tggatgataa gaaaagcttc
    49441 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49501 gacctgatca agcgtctaca acctgactgc ttcgaagata tgatcgccct agtggcactg
    49561 ttccgccccg gtccgttgca atcagggatg gtggataact ttatcgaccg taaacatggt
    49621 cgtgaagaga tctcctatcc ggacgtacag tggcagcatg aaagcctgaa accggtactg
    49681 gagccaacct acggcattat cctgtatcag gaacaggtca tgcagattgc gcaggtgctt
    49741 tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa gaaaaagccg
    49801 gaagagatgg ctaagcaacg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49861 gctgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgctggtta cggatttaac
    49921 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcacac
    49981 tatcctgcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    50041 gtgggtctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    50101 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg
    50161 atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    50221 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt gaaccgtcgc
    50281 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    50341 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    50401 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    50461 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50521 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50581 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg
    50641 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50701 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50761 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50821 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50881 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50941 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    51001 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    51061 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    51121 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    51181 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    51241 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    51301 aaaatcttcg ccgatcttgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt
    51361 ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    51421 cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtcttga cggtcgtccg
    51481 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51541 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51601 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatccggg cgtgggcgca
    51661 gaagaacgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgcctcggt
    51721 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggcgcgct ggcgattggc
    51781 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51841 tgtgcgtcca ttctgtggaa gagcgctgac aaagcgccgc tggcagctga agcgatgggt
    51901 atcatcgctc cgcgtctgaa agaactaaaa ctgatcgact ccatcatccc ggaaccgctg
    51961 ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    52021 gatctggccg atctcgacgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    52081 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    52141 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    52201 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    52261 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    52321 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    52381 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    52441 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52501 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52561 acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg tttacctacg
    52621 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52681 aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc aaggctgatg
    52741 tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag
    52801 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggaa
    52861 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52921 tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag
    52981 tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg cgccgtgaat
    53041 ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa tggccggttc
    53101 atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac tggatcaaac
    53161 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac acccattttc
    53221 atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct
    53281 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca
    53341 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    53401 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    53461 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53521 agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53581 atgaagccga atgctggccc gttgcgctag gcgaacagtg gcacggcttt aacgatgcgg
    53641 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53701 aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa ttcctcgacg
    53761 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53821 gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aaacgctctt
    53881 acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa gatcccgatt
    53941 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    54001 acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    54061 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    54121 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    54181 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    54241 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    54301 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    54361 ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    54421 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    54481 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54541 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54601 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54661 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54721 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54781 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54841 aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc
    54901 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54961 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    55021 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    55081 tggtcgaacg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag gcgcggcagg
    55141 cgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac
    55201 atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc ggccctgccg
    55261 ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc
    55321 tcgcccgcac gcggggagaa ctggagcagt gggcattggc gcatggttta cgctggattg
    55381 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    55441 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccgcacttt
    55501 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55561 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgccgcgcgg
    55621 cgattatccg tcgctggctg gcagggcaga atgcaccaat gccttcccgc gacgcgctgg
    55681 tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt ttacgcttgg
    55741 gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55801 aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55861 tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55921 tcagcgtgcg tttcaaagcg gcaggattgc tgcatattgt cgggcgcaac ggcggacgta
    55981 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    56041 tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    56101 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    56161 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc gctcaccacc
    56221 accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag ctcacgggtt
    56281 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcgctggct
    56341 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    56401 gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac
    56461 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56521 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56581 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56641 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56701 cgaagataaa ctctgtttcg caaggtgact ataccacact catgtctgca atatcagcgc
    56761 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56821 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56881 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56941 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    57001 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    57061 gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc cgcagaagtg
    57121 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    57181 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    57241 agcgactttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg cgtgatctgg
    57301 ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca accttcatga
    57361 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    57421 cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat ctgcgttttg
    57481 acattcgggc gtccagcctg ccagagtatt acaaagagcg tctactcgcc gccagccatc
    57541 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57601 tgaaccgcga agccgcgcta gcccggctgg tggcagtgat taaagattta acaacagaac
    57661 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57721 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57781 aagaaggaat agatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57841 actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc acaggctgcc
    57901 gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga ttgcgaagga
    57961 atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    58021 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    58081 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    58141 ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta tacgctggaa
    58201 ccggcacaat ctagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    58261 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    58321 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    58381 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    58441 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58501 taaacctgct taaggcagaa acaaacgcaa
//
LOCUS       DAZFDP010000011        58005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFDP010000011 DAZFDP010000000
VERSION     DAZFDP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..408)
                     /locus_tag="ACHJ1G_000773"
     CDS             complement(<1..408)
                     /locus_tag="ACHJ1G_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4762675.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMR"
     gene            416..917
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_000774"
                     /pseudo
     CDS             416..917
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(935..1603)
                     /gene="ydcL"
                     /locus_tag="ACHJ1G_000775"
     CDS             complement(935..1603)
                     /gene="ydcL"
                     /locus_tag="ACHJ1G_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4762676.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1906..2499)
                     /gene="tehB"
                     /locus_tag="ACHJ1G_000776"
     CDS             complement(1906..2499)
                     /gene="tehB"
                     /locus_tag="ACHJ1G_000776"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4762677.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2496..3488)
                     /gene="tehA"
                     /locus_tag="ACHJ1G_000777"
     CDS             complement(2496..3488)
                     /gene="tehA"
                     /locus_tag="ACHJ1G_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4762678.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3612..4592
                     /gene="ydcK"
                     /locus_tag="ACHJ1G_000778"
     CDS             3612..4592
                     /gene="ydcK"
                     /locus_tag="ACHJ1G_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4762679.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4587..5123)
                     /gene="rimL"
                     /locus_tag="ACHJ1G_000779"
     CDS             complement(4587..5123)
                     /gene="rimL"
                     /locus_tag="ACHJ1G_000779"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO4762680.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5186..5410)
                     /gene="ydcH"
                     /locus_tag="ACHJ1G_000780"
     CDS             complement(5186..5410)
                     /gene="ydcH"
                     /locus_tag="ACHJ1G_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4762681.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5550..7205)
                     /gene="opgD"
                     /locus_tag="ACHJ1G_000781"
                     /gene_synonym="mdoD"
     CDS             complement(5550..7205)
                     /gene="opgD"
                     /locus_tag="ACHJ1G_000781"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4762682.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7430..8773)
                     /gene="ydcJ"
                     /locus_tag="ACHJ1G_000782"
     CDS             complement(7430..8773)
                     /gene="ydcJ"
                     /locus_tag="ACHJ1G_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4762683.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8990..9913
                     /gene="ydcI"
                     /locus_tag="ACHJ1G_000783"
     CDS             8990..9913
                     /gene="ydcI"
                     /locus_tag="ACHJ1G_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4762684.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9951..11591)
                     /gene="trg"
                     /locus_tag="ACHJ1G_000784"
     CDS             complement(9951..11591)
                     /gene="trg"
                     /locus_tag="ACHJ1G_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4762685.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11990..12139
                     /gene="hokB"
                     /locus_tag="ACHJ1G_000785"
     CDS             11990..12139
                     /gene="hokB"
                     /locus_tag="ACHJ1G_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4762686.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(12117..12185)
                     /gene="yncP"
                     /locus_tag="ACHJ1G_000786"
     CDS             complement(12117..12185)
                     /gene="yncP"
                     /locus_tag="ACHJ1G_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4762687.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(12211..12384)
                     /locus_tag="ACHJ1G_000787"
     CDS             complement(12211..12384)
                     /locus_tag="ACHJ1G_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762688.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12629..13159)
                     /gene="cybB"
                     /locus_tag="ACHJ1G_000788"
     CDS             complement(12629..13159)
                     /gene="cybB"
                     /locus_tag="ACHJ1G_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4762689.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13348..14349
                     /gene="gap"
                     /locus_tag="ACHJ1G_000789"
     CDS             13348..14349
                     /gene="gap"
                     /locus_tag="ACHJ1G_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4762690.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14391..15830)
                     /gene="aldA"
                     /locus_tag="ACHJ1G_000790"
     CDS             complement(14391..15830)
                     /gene="aldA"
                     /locus_tag="ACHJ1G_000790"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4762691.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(16027..16827)
                     /gene="ydcF"
                     /locus_tag="ACHJ1G_000791"
     CDS             complement(16027..16827)
                     /gene="ydcF"
                     /locus_tag="ACHJ1G_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4762692.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17099..21001)
                     /gene="hrpA"
                     /locus_tag="ACHJ1G_000792"
     CDS             complement(17099..21001)
                     /gene="hrpA"
                     /locus_tag="ACHJ1G_000792"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4762693.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21202..21807
                     /gene="azoR"
                     /locus_tag="ACHJ1G_000793"
     CDS             21202..21807
                     /gene="azoR"
                     /locus_tag="ACHJ1G_000793"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4762694.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21858..23174)
                     /gene="ynbD"
                     /locus_tag="ACHJ1G_000794"
     CDS             complement(21858..23174)
                     /gene="ynbD"
                     /locus_tag="ACHJ1G_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO4762695.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(23164..24921)
                     /gene="ynbC"
                     /locus_tag="ACHJ1G_000795"
     CDS             complement(23164..24921)
                     /gene="ynbC"
                     /locus_tag="ACHJ1G_000795"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4762696.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGSRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24937..25833)
                     /gene="ynbB"
                     /locus_tag="ACHJ1G_000796"
     CDS             complement(24937..25833)
                     /gene="ynbB"
                     /locus_tag="ACHJ1G_000796"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4762697.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25833..26438)
                     /gene="ynbA"
                     /locus_tag="ACHJ1G_000797"
     CDS             complement(25833..26438)
                     /gene="ynbA"
                     /locus_tag="ACHJ1G_000797"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4762698.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26609..28915)
                     /gene="ydbD"
                     /locus_tag="ACHJ1G_000798"
     CDS             complement(26609..28915)
                     /gene="ydbD"
                     /locus_tag="ACHJ1G_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO4762699.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28979..29839)
                     /gene="pdxI"
                     /locus_tag="ACHJ1G_000799"
     CDS             complement(28979..29839)
                     /gene="pdxI"
                     /locus_tag="ACHJ1G_000799"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4762700.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(30070..30660)
                     /gene="paaY"
                     /locus_tag="ACHJ1G_000800"
     CDS             complement(30070..30660)
                     /gene="paaY"
                     /locus_tag="ACHJ1G_000800"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO4762701.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30642..31592)
                     /gene="paaX"
                     /locus_tag="ACHJ1G_000801"
     CDS             complement(30642..31592)
                     /gene="paaX"
                     /locus_tag="ACHJ1G_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO4762702.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31693..33006)
                     /gene="paaK"
                     /locus_tag="ACHJ1G_000802"
     CDS             complement(31693..33006)
                     /gene="paaK"
                     /locus_tag="ACHJ1G_000802"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO4762703.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(33033..34238)
                     /gene="paaJ"
                     /locus_tag="ACHJ1G_000803"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(33033..34238)
                     /gene="paaJ"
                     /locus_tag="ACHJ1G_000803"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO4762704.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELYRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34238..34660)
                     /gene="paaI"
                     /locus_tag="ACHJ1G_000804"
     CDS             complement(34238..34660)
                     /gene="paaI"
                     /locus_tag="ACHJ1G_000804"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO4762705.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34650..36077)
                     /gene="paaH"
                     /locus_tag="ACHJ1G_000805"
     CDS             complement(34650..36077)
                     /gene="paaH"
                     /locus_tag="ACHJ1G_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO4762706.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(36079..36867)
                     /gene="paaG"
                     /locus_tag="ACHJ1G_000806"
     CDS             complement(36079..36867)
                     /gene="paaG"
                     /locus_tag="ACHJ1G_000806"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO4762707.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36867..37634)
                     /gene="paaF"
                     /locus_tag="ACHJ1G_000807"
     CDS             complement(36867..37634)
                     /gene="paaF"
                     /locus_tag="ACHJ1G_000807"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO4762708.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37631..38701)
                     /gene="paaE"
                     /locus_tag="ACHJ1G_000808"
     CDS             complement(37631..38701)
                     /gene="paaE"
                     /locus_tag="ACHJ1G_000808"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO4762709.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38709..39206)
                     /gene="paaD"
                     /locus_tag="ACHJ1G_000809"
     CDS             complement(38709..39206)
                     /gene="paaD"
                     /locus_tag="ACHJ1G_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO4762710.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(39221..39967)
                     /gene="paaC"
                     /locus_tag="ACHJ1G_000810"
     CDS             complement(39221..39967)
                     /gene="paaC"
                     /locus_tag="ACHJ1G_000810"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO4762711.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39976..40263)
                     /gene="paaB"
                     /locus_tag="ACHJ1G_000811"
     CDS             complement(39976..40263)
                     /gene="paaB"
                     /locus_tag="ACHJ1G_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO4762712.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40275..41204)
                     /gene="paaA"
                     /locus_tag="ACHJ1G_000812"
     CDS             complement(40275..41204)
                     /gene="paaA"
                     /locus_tag="ACHJ1G_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO4762713.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41489..43534
                     /gene="paaZ"
                     /locus_tag="ACHJ1G_000813"
     CDS             41489..43534
                     /gene="paaZ"
                     /locus_tag="ACHJ1G_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO4762714.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43782..46055
                     /gene="tynA"
                     /locus_tag="ACHJ1G_000814"
     CDS             43782..46055
                     /gene="tynA"
                     /locus_tag="ACHJ1G_000814"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO4762715.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(46113..47612)
                     /gene="feaB"
                     /locus_tag="ACHJ1G_000815"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(46113..47612)
                     /gene="feaB"
                     /locus_tag="ACHJ1G_000815"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO4762716.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47848..48753
                     /gene="feaR"
                     /locus_tag="ACHJ1G_000816"
     CDS             47848..48753
                     /gene="feaR"
                     /locus_tag="ACHJ1G_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO4762717.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48925..49254)
                     /gene="ydbL"
                     /locus_tag="ACHJ1G_000817"
     CDS             complement(48925..49254)
                     /gene="ydbL"
                     /locus_tag="ACHJ1G_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4762718.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49259..49444)
                     /gene="ynbE"
                     /locus_tag="ACHJ1G_000818"
     CDS             complement(49259..49444)
                     /gene="ynbE"
                     /locus_tag="ACHJ1G_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4762719.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49441..52080)
                     /gene="ydbH"
                     /locus_tag="ACHJ1G_000819"
     CDS             complement(49441..52080)
                     /gene="ydbH"
                     /locus_tag="ACHJ1G_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4762720.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            52288..53277
                     /gene="ldhA"
                     /locus_tag="ACHJ1G_000820"
     CDS             52288..53277
                     /gene="ldhA"
                     /locus_tag="ACHJ1G_000820"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4762721.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53388..53810
                     /gene="hslJ"
                     /locus_tag="ACHJ1G_000821"
     CDS             53388..53810
                     /gene="hslJ"
                     /locus_tag="ACHJ1G_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4762722.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53807..54073)
                     /gene="ydbJ"
                     /locus_tag="ACHJ1G_000822"
     CDS             complement(53807..54073)
                     /gene="ydbJ"
                     /locus_tag="ACHJ1G_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4762723.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54347..57871
                     /gene="nifJ"
                     /locus_tag="ACHJ1G_000823"
     CDS             54347..57871
                     /gene="nifJ"
                     /locus_tag="ACHJ1G_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4762724.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14652 a        15238 c        13818 g        14297 t
ORIGIN      
        1 gcgcatccag cccagtttcg gcagaaaaat acggctgttt tcctgatcga gcttaacgcc
       61 ctgcgggtag cggaatgcat cattctgtcc ccgctttttg aatcggggaa aagcagcccg
      121 cttccggaag aagtttttgt aagcccgctc aaggtctttc agtgactgtt gcaatggctg
      181 tgagggagaa tctttaagcc attgcgtttc agtggcattt ttccactcaa ccagccagga
      241 agccattttc ccgtaaggga tgtatttatt accggcctca tgattctcat tctgacgtgc
      301 cagagcacga ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt cacactcctg
      361 ttgaccacct ggtcttaact ggaatttaaa tgcctgtaat cgtttcacag gtcaaatgat
      421 actttggtct gtgaataaag aaaccgatat ccggcgtgga aggcattgtg ttttcctgat
      481 gcatgttcac ctggtctttg tcaccagata ccgacgccag attttttgac gcgacagaaa
      541 aactacgcac ttacttttca aatgtatgtg cttattttga agctgaactg gttgaaatgg
      601 atggcgaacc agatcacgtc catttgttaa ttaactaacc tcccaaactg gcgatatcca
      661 gtctggtaaa cagcctcaaa ggcgtatcgg gtaggttact gcgacgagat cgaccagata
      721 ttgcagtcag gtattactac aaaggcgttt tgtggagtcc tggctatttt gccagtagct
      781 gcggaggtgc gccaatatcc gtcatccgcc aatacattga acaacagcaa acacctggtc
      841 aggtggaaaa ccgcacctta tatccccgcc ctgaaggatg gggttttacg gcacaccgga
      901 taaaaagcgc catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg
      961 tggcatcggt cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac
     1021 tttcgttatt caggtcttta ccttcgccct gacgaacgac tttgataacc ggtttattag
     1081 tcgctgcatc aatcagctca ccttcaaaat agaggcgagt atccatggta cggtggcctg
     1141 tagccatttg cgtccccgca accactaatg caacaggaac cacttcatag aattgcagcc
     1201 cttctttgct ggtatctaca ccggtaatgg ccccacggaa aatcagacta cgcggcccag
     1261 cggtggtaac cagtggttta cgctgcgcta tcgcttcttt catttcggtg ttggtatagt
     1321 tcaaaatttt atccagaact ttctgcccta cctgggtaga aggttttggc accggataat
     1381 aagtgattgg gttccagacg atgctgtcat atttgctttg atcaaaactc gggtctaccc
     1441 aacgtaaaac aggtttacct gttgctgagg ttgtttcttt taaatcagag taattgttta
     1501 aaaaaccaga atatttatct ggctgggtga ttttagaggc acaaccagac agagccaaca
     1561 agccgcataa agctgcaact ttcgcaaatg atgtggtacg catgaatagt ttcctgagat
     1621 gattatatta ctttttaagt tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt
     1681 gttggtgaaa ataaattttt cagccccttt tcagacgaca tagtcaatgc cgaaaaaggg
     1741 gctaagtcaa tcatcagtag ttaaatttca gtaagttaag ctgctaaaaa acttcgacta
     1801 ctgtgattaa tttcacatcc cttcgataaa aaatatgctt accgaaaaga agaacagtaa
     1861 atcatctcag cgtggggaat tttcacgcgc cttattctta gcgggtcatt ttttacgtgc
     1921 cagcatcgtg gcgaaacgca gtttaatacg attaccgttg gcgtcggtgc ggtgcagctc
     1981 gccgacgtct tcattgtatt tcaccctctc ccagccttcg taatatcgac gtaattctcc
     2041 ctctttgaag gcaaacggga agccgacggt acatggataa tcagcggtat ccatcgccgc
     2101 cacaatcagg ttgtaaccac caggtttagt gcaacgttgc atattggcaa tcagccctgg
     2161 gatggtttta gcctcaagga acatcagtac cacagtcgaa agaataaaat cgtactgtct
     2221 atcaaatgtg aggttattca gatcaacgac tcgggtgtgt aaattatcca gattttcaat
     2281 ggatttaatg cgctctacat tggcgatact catggcattt ttatcccatg cgtcaacatc
     2341 ataaccattg gctgccaggt aaagactgtt acgaccattg ccacagccca gatccagcgt
     2401 tttacccggt ttaaccactt tcaccgcttc cagtacttca gagtgtgtgc gggttaattc
     2461 atatttatca gtaaaatagt tttcgtcacg aatgatcatt ctttgtcctc tgctttcatt
     2521 aaaacggcgc gctcggttct gactaacaat tttccctgca tcagaagcgc aaaagtacgg
     2581 atgagcagta ttgcaataat aaaattggta aagataaaca gcggtaccgc cagcgtatgg
     2641 aaaaatccat tttcgctgcc actccccaga tgcaaaccgg tggttgccag tgcagatacg
     2701 ccgaacgaga aactccagaa tgaagcatta aatggctggg agagatacca tggcatcaga
     2761 cgtagcataa acagcagttg cagcagtcca taaccaaaaa gcattttcgc cagcgtatca
     2821 ccctcgccgc cgttgacgct caaccaggca ctacaggcca ccagcgcagg agcgagctga
     2881 atgccgagtg atgtccgcag tgccgtgggt aattctcccg aactgcgcag acgctgcaag
     2941 atcacaggtt caaggcttag ccatgagaaa acgcctgcgc ctaaaaacac cagaccggcg
     3001 tcggtgtagc ccaacgcacc acaggccatt gcgctgataa agttgttggc aactgtcggc
     3061 agatacagtc caggcgtggt agcttcttca gggtgagatc cgcgccataa tcccgcagtt
     3121 tgccaggcgg cataagccaa ctgaaccacg acaccaaaac tgaacaggca caccgccagt
     3181 gggcgaaacc acggaacaaa accaatcgcc accagcatcg tcgttgccgg aaacaaactc
     3241 acaaaactgc tcagcactgg atggcgaact tccgccagca cgctatgcgg aaagcgtatg
     3301 agtcgggtaa taaatgcgct agttaataat ccccagatga tcatcgccag aatcaccagc
     3361 ccatccccta accagtggct gacctgccaa acctggctgg catagcgcca ggcaaatccc
     3421 atccctatcg tccccaacac aataccaaag tagcctgccg gcaaattgag cactttatcg
     3481 ctctgcatct tcttactcat tttgtttaat ttataaagta tatttgaaat gcattttatg
     3541 gaattttctc gagcatagcc agagccgcag aatttgctac ggttatagta agtcacctgt
     3601 gggaaaacgc tatgcgcaaa tatcgtctaa gtgaagaaca gcgagccttt agttatcagg
     3661 aagatggcac taaaaaaaat gtgttattac ggcagattat cgccattagc gattttaacg
     3721 atgtgatagc aggaaccgct ggcggctgga tcgatcgcga aacggtgtta gcacaggaag
     3781 gaaattgctg gatttacgac cagaatgcta ttgcgttcgg cggaacggtg atttccggta
     3841 atacgcgcat caccggcacc agcgtactgt ggggagaggt ttacgcaacg gataatgtct
     3901 ggatcgataa tagtgaaatc agccagggtg catatataag tgacggtgtc accattcatg
     3961 actcactggt ttgcgggcaa tgtagaatat ttggtcacgc cttaattaat caacattcta
     4021 tgatcgtcgc agcacaaggc ttaacgcctg accatcaact cctgctacaa atttatgatc
     4081 gcgcaagggt tagcgcctcg cgtattgtcc accaggcaca aatttatggc gatgccgtca
     4141 tccggtatgc ttttattgaa catcgtgccg aagtttttga ttttgccagt attgaaggta
     4201 atgaagagaa caacgtctgg ctatgcgatt gcgctaaagt ctacggtcac gcgcaggtga
     4261 aagctggcat agaagaagat gccattccaa cgattcatta cagttcgcag gtggcggaat
     4321 atgccattgt ggaaggtaat tgtgtgttaa aacatcatgt cttgattggc ggaaatgccg
     4381 tggtacgtgg tggaccgatt ctgctcgacg agcacgttgt aatacaaggt gagagccgta
     4441 ttacaggtgc ggtgataatt gaaaaccatg tggagttaac cgaccacgcc gtcgttgaag
     4501 cctttgatgg cgacacggtt catgtccgtg gtccaaaagt cattaacggc gaggagcgca
     4561 ttacgcgaac gccgctggca gggttattat gaatcgataa tacgcgcgta taagttcaca
     4621 tcatcatagg catcattcag gaactcagcc tgtttcaggc aaccttcaag gataaaacca
     4681 ttgcgcaaag cgacctggtt gctttgcgga ttgtccaccc gacatttgat cacgaagcgt
     4741 ctaagttcac cagactgggc gtaatgatga atcaatgcct gcagcgcctg agaaatgatc
     4801 ccctgcccct gatgagattc gtccagccag tagcctattt cagcggtttt attcagtggt
     4861 tcaatacgat taaacgagat aacgccgata agttcatctt ctttgaaaat catgaacatt
     4921 ttggcatagc cgcgttgatg caacatcaca ttaccctgca ccgtttttcg cgtgtcctct
     4981 tcactctgaa cgaactgcgg ccagtttagc gactgctgta accaggtttt atttttacag
     5041 attaactgat aaagaggagt gacgtgattt tctgcaacag catgtaattc aagtgattcg
     5101 cttactttta tcgtttcagt catcttatct ccagtcaatc catgcagttg ttaaaaaagc
     5161 cgcctggctg gcggcttagg aaagtttaca cctctttgac gctctcctgc tgcaggattt
     5221 tgagcatctc atcttttaac tgtagctttt gttttttcat gcggaccact tccgcattgt
     5281 accctcgacc gtcggaacct tcctttctgg caatttcatg atcaagttta ttgtgtttat
     5341 cgaacaagga cataaagcga ggattttcgt ttttcagacg ggatattaaa tctcgatatt
     5401 ctggaaacat acgcactccc tgttagtggc tgacaaggtg tatctgaaat acacaccatc
     5461 agattactca tttaacttag ggaaaaatgc gagcgggatc gtgtttaacc agacggcacc
     5521 tcccgaagaa ggtgccgaag aaaaaacgat taactcatca cgcggtcgtc aacatactga
     5581 cgtttatccg gcgctggcgg gaaatactga tacagccatg tttcactgat agcgtcgccc
     5641 tgacaacgca gatacatccg catatcgacc ggatcagtgg agtccgaagt cggataccag
     5701 tcaaactgaa tacgataacc atcaatgggt tcaatataga gaatttcgat ttgcttcgct
     5761 tccccactgg aaagcgtaat caccggctca atgccttttg gtgcggcagc tttcagatca
     5821 ccaccaacga aatcgacggc aaaacgacgc gcccattttt cgggatagtg ttcacctggt
     5881 gcccaacctt ccgggaaacc gcccatgccg gtacgcgtcg ccataacgcg cgctaatggg
     5941 caatgaacag gcggttgcgc actccagtac agacgatact ggaatgcaaa ctcatcaccc
     6001 gcctttacag ctttttctgg ctgccagaag cagacaatgt tatccagcgt ttcgcccgtt
     6061 gttgggattt ccatcaggcc gatggtgccc ttaccccact tgttacgcgg ttccacccac
     6121 agacttgggc gtttgttata ccagcccata atgtcctgat aatgggagaa atcacgatcc
     6181 agttgcaata aaccaaaccc tttcgggttg ttgtcggtgt aagcattgaa ctgcaatttt
     6241 tgcggattat tcagcggacg gcaaatccac tcgccgttgc cccgccacat ggacagacga
     6301 tcagagtcat gaatttgcgg atgaatggtg tcgcacatcc gacgttcatt agtaccgcag
     6361 ctgaacatac tggtcatcgg cgcaatgccc agctgtttaa tgtctttgcg cgcatacagg
     6421 tgattttcca catccataat cacctgactt ttctcacaat ggatagtgaa cttataggca
     6481 ccagtaatgc tggcgctatc gagcaacgca taaacggtaa aggtagttgc ccccggtttt
     6541 accgtatcaa accagaaggc ggtaaagtcg gggaactctt ctttactgtc ggtgtaagtg
     6601 tcaatcgcca ggccgcgggc cgacaaaccg tattgatatg tgtcatcaac ggcgcggaaa
     6661 taactcgctc cgaggaatga tactacatca cggcgcgcca gttcgggggc tttaaacacg
     6721 cgaaaaccgg caaagccgag atcgctttgc ccttctaatt gtttggtatc aacacctgca
     6781 tcgttgtatt tgaacaactc cgggcgaaag tgaatttcac gcgccagatg tgttgctgga
     6841 tctacagaaa acatacgaac gcggcgacgg aatcccattc ccatatggaa gaactgagcg
     6901 tccagttgac ggttctcaac gttatgccag agcgattttt cggcgtcgta ttgaatactg
     6961 ttataagcct gcggcgtcat tgtcgccagc gtgtcaggta acggacgagg cgcaccacgc
     7021 cacgctgttt gcgctaagtc gtgcgccatt gactgtagaa tggagaagtc aaaacgctgg
     7081 gtttgcccgt cggcaatatc agaatctgcc gcgaatgccg cctgagaaaa aagagaagca
     7141 atgccgctgg taccgcacac ggcggccata gccattgaac ctttaataaa tcgtctacga
     7201 tccatacctg aaagtgagtc cttctggtgt tgtgaatggt cccgcgctgg tcgttttcag
     7261 cggcaagaaa gtgcttattt atgaccgctc actctagaca atatttatta ataatccaat
     7321 tgttgaactc agagaatatg cataaatcgg caattttttt atgtcgatgg gatgagcctg
     7381 attcgtttgt aaaatcaggc aaatttttgc aaaggcggat ggtcagaatt taaagcaaac
     7441 cacagcgacg tttactgcgt tcttccgctt cctgataaag ctggaactca tccaaaaccg
     7501 gacaacccaa cgcctgctca aatgcctcgc gactggcatt accgtgattg cgtgcctgcg
     7561 tttcattacc cagatttgac tggaagatcc ccgccgcgct aacgggcaag aaatcttcat
     7621 aggtaatggg ttgcgccgcg acccaaccac gttcaattaa gggctgtgga tcgtctccgg
     7681 gatgaatcgc ctgacgatgc gcctcacctg aaggcgtcag acggtaccgg aaccatgcca
     7741 gcccttgctg acgcattaaa aactcactgt caggaaaagt gcggaaggtt tcctgtaaat
     7801 gcatttggtg agtgagatta tcctgcccgg ttccagcgtt acgcagaaga tcatcataca
     7861 gttgtcgccc tttcggcgtt aatgccacgc cacgctgctc aatttcacca aagcgcgcgg
     7921 tatgcgtgcc ctgtttctgc cccgcaaaca acaccgtctc ttccagtgct ttaaagctgg
     7981 tctggcgtag taaaatcggt acctcgcggc gcggcggccc ctcgatgaga attttgggtt
     8041 caattccgca ttcaggcatc atcgactgca cccggtcaat atccagcgta cgtggcgtca
     8101 ggtggttgat atggcatcca ggaaaacaga ccacatcagc aatcaaccga tgttcgttgt
     8161 gcaaggcgcg ataggtttct tcatctaccg ttgctgactg gtgccagcga aacgtttcca
     8221 gggcttcctg cacaaactcc tgtgcctgtg tttcgttaaa accgccctgc tgctcatatt
     8281 cctctaacag ttgtcgacaa cgtggggtga agatatcgcg ctgacgcaga atctccgccg
     8341 ctttctggcg caaaatttcg ttctcgataa gctcaaggcg gagtaaggag gtaaaaacgc
     8401 ggaagggatt acgcgccaga gaagcatcat caatgggccg aaatgctgtc gaatgtaccg
     8461 gcacccctgc ctgcgagaga tcgtaatagc tcaccgggta catccccata atggcaaaca
     8521 tccgccgaag agtagcaagc tcttgtgcag tcccaacgcg aatcgcccca tgacgttcaa
     8581 cattcagtcg cgccagctcg tctgcattta ccattttttc gtgcagttga ggattgtttt
     8641 ccagcacagc cagattcaca tcagctacca gttccagcag cgtgccatat tgcggaactt
     8701 cttgctggta catggctgac attgcctgcg aaaactgttc ccgaatctca tccgccgtga
     8761 tgctgttcgc catagtgtct gcctccagtg gtcagtaatc tggagtgtag gtaaccgcat
     8821 tcactctggc gggaagaatt tacaaactgt gatctcgccg cgaaaacatc aatattatcc
     8881 attttgctgt aacacaattg ctttaattgt taataatatt ttgcaatcaa gttatcataa
     8941 tcaaacaact tcacttgtca gcgacaccgc ttcgttttta acatcgctta tggaaaaaaa
     9001 tagtctgttt agtcagcgca tccgtttgcg ccaccttcat acattcgtag ctgtcgcaca
     9061 acaaggaact ttggggcgcg cggctgaaac ccttaatttg agtcaacctg cgctctctaa
     9121 gacattgaat gaactggagc agctgactgg cgctcgcttg tttgagcgtg gtcgtcaggg
     9181 ggcgcaactt accttacccg gcgaacaatt tttaacgcat gcagtcagag ttcttgacgc
     9241 catcaacact gccggacagt cgcttcatcg taaagaaggt cttaataatg atgtcgtcag
     9301 ggttggtgca ctacctactg cggcactggg gatattacct tcggttatag gtcagtttca
     9361 tcagcaacaa aaagagacga ccttgcaagt tgcgacaatg agtaacccta tgattctggc
     9421 gggtttaaaa accggggaaa tcgatatcgg cattggtcgg atgtcagatc ctgaactgat
     9481 gaccgggctt aattacgaac tgctgtttct tgaatcgttg aagctggttg tccgccctaa
     9541 tcacccgcta cttcaggaga acgtaacgct aagccgggtg ctggaatggc cggtcgttgt
     9601 atcaccagaa ggcactgcgc cacgccagca ttcagatgca ttagtgcaga gccagggctg
     9661 taaaattcct tcgggttgta tcgaaacgct gtctgcttcg ctatctcgtc aacttacggt
     9721 tgaatacgat tacgtgtggt ttgtcccttc tggcgctgta aaagacgacc tgcgtcatgc
     9781 cacgctggtg gccctgcctg ttccgggaca tggtgcaggc gaaccgattg gaatactgac
     9841 ccgcgtagat gcgacgttct cttctggttg ccagttgatg attaacgcta ttcgaaaatc
     9901 aatgccgttc tgaaaggtga agggatctgt cgatccctcc ttgaacattt tcacaccgta
     9961 gcgaaactaa ctggttcacc cgctccgcga ggttctgccg acacagaatg tttgtgcaga
    10021 cggaatacat ccaccgcctc agttaatcgt gccgcctgtt cttcaaggga caccgccgcc
    10081 gctgaggcct cttctaccag agaagcattc tgttgcgtca ccttatccat ttcagaaatc
    10141 gcctggctaa cctgcgttat gcctctactt tgttcatccg aggcggcggc gatttcctgc
    10201 atgatatgtg tgacactcgc gacggcatca acaatagtgc tcatggtttt ccctgccgtt
    10261 gccacctcat ccgaccccag gtcaattaac ctgactgatt cactgatcaa gccttcaatc
    10321 tctttcgccg cttgggcgct gcggcttgcg agtgtccgta cttcgctggc gacaacggca
    10381 aatccacggc cttgctcacc cgctcgcgcg gcttcaacgg cagcattcag tgccagaata
    10441 ttcgtctgga aagcaatact gttgatgacg gcggtgatct cggaaatttt cttcgaactt
    10501 gtagagatag cgcccatcgt ttttactaca ccggaaaccg tctgcccgcc atcgctggct
    10561 ttaatagaag cctcttgcgc cagtttgctg gcatgatgcg cgttatccgc attctgtttc
    10621 accgtcgcag tgagttgctc catgctagcg gcagtttgtt cgatagccgc cgcttgttct
    10681 tcggtgcgag atgacaggtc cgcattgcca gctgaaattt cgctggtgcc acgataaatc
    10741 tcttccgcac cctgtcgaac agtccctact gtcatcccca gtgaatgctg catttgctgt
    10801 aaatgacgac ttaagcgacc gatttcatta cgacccgccg gttcatcatt catcgtcaga
    10861 tcgccactgg cgattttttc aatccgttgt gcggcatgtt gcagtggacg aatgacgatc
    10921 cgacgtagca ccataaatgt tatcagcgtc atgaccaggg caagcacaaa cgcgccaatc
    10981 atgaacatcc cacccaggcg ggtgcgctga tgggccagtt ccgccagttg gttggctctg
    11041 gtgctacgta tcttaacggc tttgttcaaa atatcggtat aagcattatc cagcggtcgg
    11101 atctgctcac tttcatgatt gataatcgct tcaaacatgc catttttggc atatttcaac
    11161 ataggttgca tacccgtgat ataagcctga aagcgttgat ttaattcagt gtcgagggct
    11221 tcatcagcag gtgttttcac cggtcgattc tgataagcac gataaccttg ctgcgactgt
    11281 ttaatctccg attcggcttg cgcaatattt cgcttcattg cttccatttc cgcaatacga
    11341 cttgcagccc cggcctgaat catattgatc cgggctgaac gcaaaaaatc tgaactgttc
    11401 gctaacccgg tccgaatctc aatctccgca gtaacatcac gctgatcgcg atcggcctgc
    11461 cagaggaaat agccagccag agcagaactt aatgcgaata gaactaagat accgccaaga
    11521 atgcaggcaa ataacggaac cagcctgatg tgatgcaaaa aacctaatcg atgtgaggga
    11581 gttgtattca ttaccgttgt ctctcgtcca ggtttactcc tgatagaaag tcatcggcac
    11641 ttgacggtaa ttacttatga ccaaaaacct ctttgcgtca catttttaca acaattattg
    11701 cagagaaaat taatgctatt ggaatgaaga gagtaacgaa tggctaaact ggctaacccg
    11761 aatgacgaaa aattctggaa aacagcacgc aatttcgtga agtgattatt ttcgtcccta
    11821 aggaaacctc ataggatgcc tgcgggtgcc tgagactttc tgttttcagg ctaaccggga
    11881 atcagaaaga cgaaggcccc gaccgatatt tctatcaacc gaggccaacg aatgaaccta
    11941 gcaattcaga tcttagcctc ttacccgcca tccggcaagg agaaaggcta tgaagcacaa
    12001 ccctctggtg gtgtgtctgc tcattatctg cattacgatt ctgacattca cactcctgac
    12061 ccgacaaacg ctctacgaac tgcggttccg ggacggtgat aaggaggttg ctgcgctcat
    12121 ggcctgcacg tccaggtaag ggcaagcgcg gggattttcc ccgcgcatct gactagcaga
    12181 ttcattttct catggcaccc cttttacact tcagttagtg catgtttttt tcgaggcact
    12241 aatagaacca tcattacaga caaatttgcc gcctgccgta cagtgtgaga tccccccttt
    12301 cgaaccagaa cagggtttgc gcccggcatc ggcataaaag gaaacaagcg ttcccataaa
    12361 caaaataagt gccagttttt tcatataacc tccatggttt atcgaatctt atgttcgatt
    12421 tgaacgttat acgctggttt attttgatac cgttgatgag atcaaagatc gggtgaattg
    12481 attaaaaata agccagatgg acgtatgggc aaggattatg gttttatttg tcatacaaat
    12541 aaatataata ggcgcttccg atgtagaccc gtattcttcg cctgtaccac gggtcggttt
    12601 tagtacaggc gttttcttta aatatccttc aggaacgttt acgcggcatc atgcgtagaa
    12661 gtgtgttgtc cttccagaaa tagtggtgtg ccagtgcagc cgcagcgtgc aacccgatga
    12721 caaaatatcc cagattcgcc agcgtctcat gccacgactt taagctatca acccgttcga
    12781 aattggcctc tgaagcgtaa ggcatcgtca aaccaaacgc aaaccacggg ttgccccggt
    12841 tatacatcat caccaaacca atcactggca gcgcaataaa caggagataa atcaccaaat
    12901 gtcccaaatg cgccagtccc gtcatcatcg gttttggctt aggtataatc ggcggggttg
    12961 ggtatttcag tcttaacaaa agacgaacca ccatcagcac gagaattgag ataccacagg
    13021 aaacatgaac catgttgatg agtggccgat cgctacgtgg gaagaaacca cgaaactcca
    13081 ttgcgcaata cgctgcgata accagcaaaa agaccagcca gtgaatgctg atttgtaacc
    13141 ttgagtattt attttccata acatttcctg ctttaacata attttccgtt aacataacgg
    13201 gcttttctca aaatttcatt aaatattgtt cacccgtttt cagtacgtta cgtttcactg
    13261 taagaatgga gattgcccat cccacaatcc tggtctaagc ctggaaagga tcaattttca
    13321 tccgaatgtt cctgacagga gaaaaccatg agtaaagttg gtattaacgg ttttggtcgt
    13381 atcggtcgac tggtgttgcg tcgattactt gaagtcaaaa gcaacataga cgttgtcgct
    13441 attaatgatc tcacttcccc aaaaattctc gcctacctgc tgaaacatga ttcaaactac
    13501 ggaccattcc tctggagcgt tgattttacg gaagattcac ttatcgttga tgggaaaagt
    13561 atcgcggttt acgccgaaaa agaggctaaa aatattccgt ggaaagcgaa aggtgcagaa
    13621 atcattgtcg aatgtactgg cttttatacc tccgccgaga aatcgcaggc gcatcttgat
    13681 gctggtgcga agaaggtgtt gatttccgcc cctgccggtg aaatgaaaac tatcgtttat
    13741 aacgtcaatg acgacactct ggatggcaac gacaccattg tttccgtggc gtcatgcacc
    13801 actaactgtc ttgcgccgat ggccaaagcc ttgcatgaca gtttcgggat agaagtcggc
    13861 acgatgacga ccattcatgc ctatactggc acccagtcac tggtggatgg cccacgtggt
    13921 aaagatttac gtgcttcacg cgcagcggca gaaaatatca ttccccacac tacgggggcg
    13981 gcaaaagcca ttggtctggt gatcccggaa ctgagcggca aactgaaagg tcatgcgcaa
    14041 cgcgtgccgg tgaaaacagg ttcggtcact gagctggtgt ccattctcgg aaaaaaagtg
    14101 actgccgaag aggtgaataa cgcacttaaa caagcaacca ccaataacga gtcatttggt
    14161 tataccgatg aagaaatagt ctcttccgat atcattggca gccatttcgg ttcggtgttt
    14221 gatgccacgc aaacggaaat taccgccgtg ggcgatttac aactggtgaa aacggtcgcc
    14281 tggtacgata acgaatatgg cttcgtcacg cagcttattc gcaccctcga aaaattcgct
    14341 aaactctgac gcgcacaggc ggaggaaaaa acctccgcct cttttactca ttacgactgt
    14401 aaataaacca cctgggtctg cagatattca tgcaggccat gtttaccatc tgcgccgcca
    14461 ataccggatt tacgccatcc ggcgtggaag ccttgcatag cttcgaagtt ttcacggttg
    14521 atgtaagttt caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg
    14581 gtatagattg atgaggtcag gccgtaatca ctgtcattag ccattgagat agcatcttcc
    14641 agcgtgtcaa atgcgaccac cggcagaacc gggccaaagg tttcctcatg cataatcgac
    14701 atttcctggc gaacatccag cagcaatgtc ggcggataat aatatccttt cccgtctact
    14761 gctttgccac ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctcg
    14821 accctttcca gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg
    14881 gggttaccaa attgaaccgc ctgcatcgct tcacccagcc gattgacgaa ctgatcataa
    14941 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg
    15001 cgtgaatcaa cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagct
    15061 ggtgctttac cacccaattc caggcacact ttggtgatgt ttttcgccgc agtcgccata
    15121 atcttctcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
    15181 gccagttctt gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga
    15241 aggcctattt catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg
    15301 ggtttaatga cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg
    15361 aagaacggga agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaaa
    15421 agaatatttt ctcctggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac
    15481 tccgccatgt aatcgatata gtcggcagta aaagccactt cgacttcagc cagctgctgg
    15541 atcttgcccc cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatgccg
    15601 gcggagattt tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
    15661 tgtgcacgtt ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtatg
    15721 cgggaaatga cagcctctgc agcagggttt accacatcaa tccatgcgtc tccacgccag
    15781 gtaacaaact gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactcc
    15841 tgtgatttat atgtttgttt tcggtgaatg cattgttaat taacatcaat catcagagag
    15901 acggcatcgt gaaaggacaa ttctgtgaag ggcttcataa aaacaagtgt aaaaggaata
    15961 aaaggcagaa ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg
    16021 caatttttaa cgtaacgagc gactttccat cgcctcgatg agcaccgcat catgtttcag
    16081 cgtttgccat gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc
    16141 gcggggaccg tagccatcgc tatcatcgcg taaacgcggc agctcgccag tgagtagtga
    16201 gagataacgc tcaaccggcc ataatccttg taactgatta ataaagatta cactgtctgc
    16261 gttatttcct aactgaggaa cgaatccggg ataacttaac cagcgtggtg catcgggatt
    16321 gtccccagtc atacggcgga acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac
    16381 aacgatagcc gtatgaactc gttctacggc ctgattcaat agcgcgatgc taaagcgtgc
    16441 gttttcaccg cagtttgttg actggtcttc aatccagatt ttttcatgcg gaatgtgcca
    16501 gaactgatga gcgatatccg ccaggatggt cgcttctgct ctgccagtgg tgcggatagt
    16561 gttgtagtgc ggatgctgtg cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc
    16621 accactaatc agtaaaggaa tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt
    16681 cggcataacc gcattgcctg caaggatcac gcaatcggcc tgatacggca cctcaccgga
    16741 gaaatcatcc tgcgccagcc actgtccgat aacatttatg gcatctatag ttgccggcga
    16801 aagcgtcgga aacggggtaa tgttcatggt tcctccttat cgttccctta agaatactgc
    16861 gtgagataat agtgagaagc ggagcactct gaaaggagga agtcgtatgt ccttaaccgc
    16921 cactccgcaa gcctgacata tcttttaagg aaatgctgta gataaacatt attcatcttg
    16981 cagcagagaa ttgtattttt ctgaattcct gaatttttac ccttaccgtt ttagaattaa
    17041 taaaaaaccc ggcgctaaat gcttaccggg ttttttcttt atcaggcaaa tagcagggtt
    17101 aaccgctaat ctgctccatc gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac
    17161 caagttgttg agcgaagtaa ctaacgcgca actcttctat catccaacgg atctctttca
    17221 cgtcttcatc ctcacgacgt gcgggcggca gtttgttgat ccattgctgc cacgcctgct
    17281 ggacgttttc gactttcagc atctgagcac ggtcgcgatg tggatcaacc gccagttttt
    17341 ccagccgttt ttcaatcgcc tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt
    17401 taccagtgac aaaaccgcga tataccaacc cgcccatctg cgctttaatg tcagaaagcc
    17461 ccagtgccat ggtcatatcc acccgccctt tcagacgttt gttgatattg aacaccgcag
    17521 tgaggatttg ctcgacctgc ttcgcaatat ccaccaccgt gtcattcagt tcggcgcgca
    17581 ctttttcatg cagcgcagca aagccttctt ccgtccagac cgggccacca ttggcgtcga
    17641 tcaattgatc cacaccgcag gagatacagt cgtcgatcag ctccagcact ttgccatacg
    17701 ggttaaagta cagtcccagc ttggctttgt tcggtaactt ttcatgtaaa tatttgattg
    17761 gcgatggaat attcagcagc agtagacggc gaagaccgtt ccacattgcc tgcttttgct
    17821 ccagcgggtt atcaaacagt ttgatcgcca cactgtcgcg ctcatccacc agcgccggcc
    17881 acgccttcac tttgtagttg ccacgcttct gttcgtagct ttccggcagc tgaccaaaac
    17941 tccagatatg taagccgctc tgctcgatac cgtcatccgc caccgcagat agcgtttcct
    18001 gcactttgcc tttcagcgca tctttcagat cttgtagcga gcgcccttct tttagcttct
    18061 tgtttttgtc atccaccacg cggaaggtaa ttttcaggtg atcaggcacc tgatcccagt
    18121 gccagtcttc gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt
    18181 cgagcaacgg taactccagc ggtttgacgc ggcctaaaaa cgcttcggca tagtttggcg
    18241 cgggtacaaa attacggcgt accggtttcg gcaacgattt aatcagagca atcaccagtt
    18301 cgcggcgcag accggggatc tgccattcaa acccgctttc ctcaacctgg ttaagtaacg
    18361 gcagcggaat atgtacggtc acaccgtcag catccgcgcc gggctcaaac tgatagctca
    18421 aacgcagctt gagattgccc tgatgccaga agttcgggta atccagcttg ctgatttttt
    18481 ctgcgccctc tttgatcaac atgctttttt caaagttgag caaatcaggg gtttcgcggc
    18541 tgactttttt ccaccagctg tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct
    18601 ggtcgtagaa ctcaaacaac gtttcgtcat caaccagaat atcgcggcga cgtgatttgt
    18661 gttccagctc ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg
    18721 tctgccagtc accttccacc agcgcatggc gaataaagag ttcacggcat aacgccggat
    18781 cgatctggct gtagttgacc ttgcgcgcgg caacaatcgg caaaccataa acagtgactt
    18841 tttccgttgc catcaccgcg ccctgcgccc gttcccagtg aggttcgctg taggtgcgtt
    18901 taatcaaatg ctgagcgaca ggctccaccc attccgggtc gatacgcgca gcaatgcgcc
    18961 cccacaggcg gctggtttct accagttccg ccaccattac ccatttcggc ggttttttga
    19021 ataaaccaga accgggaaag atggagaaac gcgcgttacg tgcgccggta tattcttgtt
    19081 tatcggcatc tttcatgccg atatgggaaa gtaaacccgt cagcaacgca atgtgaattt
    19141 cgcgatactc cgccggttcg ctgttaaccg gaatgccaag ttctttcacc acctggcgca
    19201 actgggtgta gatatcctgc cattcgcgca cgcgcagata gttgagataa tcgatacgac
    19261 acaggcgacg gaaggcgttg gaagaaagcg ccttttgctg ctcgccaaga taattccaca
    19321 gattcacaaa cgcgaggaag tcagactctt tgtcgtggaa gcgacgatgt ttttcgtccg
    19381 atgcctgctg tttatccatc ggacgctcgc gcggatcctg aatggaaagc gcggacgtga
    19441 taatcatcgc ctcacgcacg cagccatgtt tttgcgcttc cagcaccata cgcgccagac
    19501 gtggatcgac aggcaactgc gagagctggc gaccgagcgg cgtcagttta taggcgctgg
    19561 cctgttcatc cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca tcctggatat
    19621 tgcgtttatc cggtgcttcg acaaacggga acgcagcgat atcgcccagc cccagcgcgg
    19681 tcatctgcaa aataaccgag gccaggttgg tacgcagaat ctccggatcg gtaaactccg
    19741 ggcgcgaaag gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgcc
    19801 cacagcggcc tttacgctga ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct
    19861 gcactttggt gcgatagctg tagcggctga tacgtgctgt accggggtcg ataacgtatt
    19921 taatccccgg tacggtcagc gaggtttccg cgacgttggt cgccagcaca atgcgccgtc
    19981 cgctgtgcga ctggaacacg cggttctgtt cgctgttcga aagccgcgca taaagcggca
    20041 agatttcggt atgacgcaga tttagcttgt tcagcgcatc ggcggtatcg cgaatttccc
    20101 gctcaccact cataaagatc agaatgtcgc cagggctttc ctgactcagt tcgtctacgg
    20161 cgtcaaaaat cgcctgcaac tgatcgcgct cggtgtcgtc ggcttcttca acaatcggtc
    20221 gatagcgcac ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa
    20281 agtggcgcga aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaggtcag
    20341 gacgccgcgg cagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt
    20401 cgtgtgcttc gtcaataatg atggtgtcgt actgcatcag caggcggtct tgctggatct
    20461 ccgccagcag gataccgtcg gtcatcagct tgaccatcgt gttatcacta acgtgatcgc
    20521 tgaaacgcac tttgtaaccg atgcaaccgc ccggctccgt tttcagctct tccgcaatac
    20581 ggttcgccac cgttcttgcc gccaggcgac gcggctgggt atggccgatc agccctttaa
    20641 tcccgcgccc cagctccata cagattttcg gtaactgagt cgttttacca gaacccgttt
    20701 ccccggcgac gatcaccacc tggtgatcac gaatcgcttc gagaatgtcc tgttttttct
    20761 gactaaccgg taaattgtca ggataagtaa tttccggtcg tgccgcttca cgcagcagga
    20821 ctttccctgc cgcctggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat
    20881 caggattttt aaccttctta acaccgtgca gacgccgaga aaaacgcagt ctgtcgcgca
    20941 gcatcagcga atccagccgc tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca
    21001 taacgttagt gggcagtagt ctgcctgatt aattttcttt taaatgatag atatagagta
    21061 ccacatcgcg gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc
    21121 gctatctctg tggcaggatt gtgaataaag tgtcagcaag caacggggca tcgcccattc
    21181 aaacatctat aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca
    21241 gggtactctc agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac
    21301 tccgctgatg aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt actggatggc
    21361 gaactggttg gtgcactgcg tccgagcgat gcgccgctga ctccgcgtca gcaggaagct
    21421 ctggcgcttt ccgatgagct gattgccgag ctgaaagccc acgacgttat cgttattgcg
    21481 gtaccgatgt ataacttcaa catctcaact cagttgaaaa actattttga cctggttgca
    21541 cgcgcaggcg ttactttccg ctataccgag aacggtccgg aaggtctggt aacgggtaaa
    21601 aaagccatcg ttattaccag ccgcggtggg atccacaaag atggaccaac ggacctggtg
    21661 acgccgtatc tgtccacgtt cctcggcttt atcggcatta ccgatgtgaa atttgtcttc
    21721 gccgaaggga tcgcatacgg tccggaaatg gcagcgaaag cgcagtctga cgcgaaagcg
    21781 gccatcgaca gcattgttgc tgcataaccg gacacgatct tgatgtggac tgtagttcag
    21841 tccacatctc aatccactta cctgttttcc cataatttca gcatcgcttt gtgatcttcc
    21901 ttaagcacaa tatgcgaacg tctggctcga atataactaa tcgcttcatc aacggtttta
    21961 cagtgtccgt aacataacaa ccatgccgcc actaccagcg cactgcgcga taatcccaac
    22021 gcgcaatgga ccagaacgct gccttgctct tcgcgtaatg tttccagcat cgccacggcc
    22081 tgtcggagct ccccctcttc cggaaccacc agatccagca tcggtacaca gaaatagagt
    22141 cgatcttttg tcgctcgtcc ccgagggaat tcaaaggtga cgtccagaac cgcattctgt
    22201 gccggaatat gtcgtggaaa cgcccctaaa taaacaccag cagtaatttt gctcaccggc
    22261 tccaggcgac gacaaaacca acgcatagac agccacatcc cgatgcgcca gggcaatgtc
    22321 agccagtaaa cggcgggcgg gagtttcccc tgactatctt tccctgttgt tatcgcgcca
    22381 agcccaccgt acccacggcc aataatgagt agcgataata ctggccaaca taaccagact
    22441 gaccaccata actgaatcat caccattagc tccatcaaca caatgcacga gcccgcgcct
    22501 acgacatatg gcagtgctat tttgattcga cgttgatcag gtttctgata attccaacga
    22561 cgatcgacgg gcaccatcca gtcaatcaac atacctaccg ccagccctgt gatgacatca
    22621 ataaaatgat gctgccaggt cgtcagagta gaaatagcga tgagtaaaaa ccatccgcca
    22681 cagactttac gccacctctc agccagatgc tgacgaaagt gacgccaaag tagccagcag
    22741 agaataatat gcagcgaagg agactggtta taaggcagat caaacagttc aagttgcgaa
    22801 aatagccatc ccgtcacccc actcacttca ggacggataa aactaaactt cagcggatag
    22861 agcaaaaaac cgcagcaggc cattaccgtt gccagaataa gccggtggac aagtcggcgc
    22921 tgttcgaatg tcgtgctaca aacgaacagc gaaaatccat ataaaagatc cagactccag
    22981 taaggaacaa tagtccaggg aagaaaaggg atcgccgttt cccaaccgaa tacctgactg
    23041 gggatatcat ggctgttaag gtcctgaacc gcggtgaact gattaagaga tccataggtg
    23101 aagaagaaaa acggggccag caacaataac cagccagcgc cttgtagcaa tacgttccga
    23161 cgttcagtta tcacgacgca ccgccatcga aaccgtaaaa ataccccact catcaatccg
    23221 ttgtgtgcat ttatcaaatc cggcatcacg cacgagtgaa tccatctccc cttgcgaacg
    23281 cacgcgcatt acccacggtt taccatcttt atgactggtt aacaccccgg caatcatctc
    23341 cagttgaggg tgccacggct gcccggtgta gatgagaatg ccgcccggtt cgatggcatt
    23401 ggcaagacct gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catacaggcc
    23461 agagacaatc gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc
    23521 atcgccctgt tcaaaacgca cccgcccaga cattccccgt tgggcaatca tcgcttgccc
    23581 ctgtgcaaca tttaactcac tgtaatcacg taacaaaata tcgcttacgg caggctcgtt
    23641 tgccagcgca tccagtacat agcgcccatg ccctgcggca atgtcaacca cgcggacggc
    23701 taaacctttg gcgtggagat cggcaacggc ttgtttaatc agtatttgta aatgggtttt
    23761 acgctggcga ataccgcgcc agccaacact gttcaggtag attttgtcaa ttaagcgccc
    23821 gaatgcgtta ctgccttgcg gttgattttg atagacataa tccagggaac tgcctgaatc
    23881 aaagccggtg ctcattccga gatgcaggcc cgcagagtgc gccccgaaca atttcatcgc
    23941 ctttcgcata aagcgatacg ccaaatcaac cggcgataat ggcacgggtc cacctgaaag
    24001 gagccgccag cgatccgctg atggtccagt gcggtcttca tgctgataat caaatttttg
    24061 tgacttgtta gcatataaac gactaataaa gctttgcatt ttttcaaatg cctgcgccct
    24121 gttctcttca cccaacgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg
    24181 gctacgtaat ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcgcc
    24241 tgatatcaga agttgcgtgg ggagcgtaat cgccgccgca tctctaataa tacgttcaga
    24301 cgttttgtag agatcgagca agatattaac ggcaatcgcc cgtgtgatca gcggatcatt
    24361 attgaaactc gccacccgtt gccgatcgtg ggtcaaatag cgtcctttca cataggaatt
    24421 aataaaaaac agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggtac
    24481 atacaattta accttaaagg ccggagaagc cagcaccagc ccgcgaattg caggcgcata
    24541 atcatgaacc catgtggcaa ccagcactgc gccgacgctt tgcgcgatca ctaccacctc
    24601 atccagtccg acctggctat cgctggcagc aaaacgaaca aattcatcga catcctgcac
    24661 tgaacgcgca agagatgggc tataaccacg cgaccccgaa gtttgtccat gccctcgggc
    24721 atcccaggca taaaaaacag tatctggcat cgccagttca tcaacgagat gttgtagacg
    24781 accagaatgt tcatgaccgc gatgaaataa gacgatgacc tttttagccc cgggctgtaa
    24841 agcgggccag tgccgataaa acaacgctgt attatcactg ctggtaaaaa aatgttcccc
    24901 atggatgcgt gaattttcca ttttctctat tttccttcag taacagcagt agcgaataaa
    24961 ataaaaaaat accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg
    25021 tcctggtaat agtttcccac tatctttaac accaatatct cgtttgatgg ctgacatcac
    25081 gacatcgccg caaaaaccac taataccaat taacaaaccc gctaataatg cctgtaatgt
    25141 atttagcggt gtcagcaacg gtccaataat cagtgacgca atcatggtgg tgattacgcc
    25201 ccccaccaga ccttccagcg tttttcccgg gctgactttg gggaccactt ttcttctgcc
    25261 gcaggatttc ccccataaat actgtgcaat atcgtttgac tcggttaatg ccaaaagaaa
    25321 taacaccagt aacgccccgc cctggatatt tgtggttggc aacattaata accaggcggc
    25381 atgactcaag gcgaaaacgg tcattatcca gccccaaaaa atggcgctca cggtatgcag
    25441 aaaaccggag gggtccccca taaaaacttg cccggtggct aatattagaa acccagccaa
    25501 aggtataaat aatagataca gctcaaagca attaaaaccg ataaacaaat agttaagagg
    25561 aataacccaa tataaccacc gcggaaaatg tacagatata agcgtacaat attctttcaa
    25621 tgccagaaaa cttatcagcg caaaaaacgt caacgtcatc cagcgtggac ctgaaatcac
    25681 cagtgaaaaa caaatgacga tgccccacca ggtacgaata cgtaatgtaa cttcatcctc
    25741 ttttctctcc ggtaaccgcc atagcagaaa atggtttatc agcgttgcta aaattaacaa
    25801 cgcgaaaagt gttgccagag atttttccag cattatcttt cagcgctcat aagaagcaca
    25861 ctccgacaac gattaatcgc agtccagaga agcagaattg aagcaatgct ccataaaaga
    25921 ttattccact gcatccactg tggataaatg gcaacggcca gaccccacag accaaatatt
    25981 aacgcgcgat cgcttttgcc aaatggaccg gcataactgc gaacaccgtt aatcgtctgc
    26041 gcgagtaaac cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat cacgagtgat
    26101 gcgttacttt ccggtaaaaa taaaaatggt aaatagagcg caatatcgga aataacatca
    26161 ccagtctcat tcaaaattgc cccaagccgt gtttgctgat tgcactcacg cgccaacatg
    26221 ccatccagcg cattgagcgc catacggata aaaagcacga tgggcaatag cataaagagg
    26281 atgggttgtg ccaccaacat caacagcaat ccggtaagaa gagaaagcgc cagtgcagca
    26341 agagtgatgt gattcgctgt aacgtggtgc ttgtaaagcc aaaacatcgt cggccttaac
    26401 agcgactgaa agagcggttt tatctggtag agcgtcatta tcaatccttg ataattcggt
    26461 ttgcgctatg gtgcatcaat tcccctgacg gagatatcgc aacacgccgc ttcattaaaa
    26521 attcagaata tatcaaggta ttggggactc actgtcagga ataattatcc ccaattaatc
    26581 aaagggttaa gaaatattac gttgtggatc aatacaactc atcaagtttt ctcatcgctt
    26641 gttcccgtat tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa tccagttcag
    26701 ggttaacaag ctctaacgcc agcaaaggat cacgtttcag cctcgccttt aatacatcaa
    26761 ctggaaaacg gggattattc atcgccacaa tccaccactc gggatcaatt tctgccgcca
    26821 gtactgcagg cggtgtgtgg cggttgttaa gtgcgccgaa cttgaaaaat gtgagattgc
    26881 ttttcgccag tgtactccac catatacgat ccggttgttt tgccagtaca tccagttgtg
    26941 ctaacgcatc ggggtcgagt gcataataga cgcgtaatgc ttcatcaacc tgcgcacggg
    27001 agctgttttc ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc gtattttgct
    27061 gtaattcctt gaggacaaca gcgggtaaat tatcccaact aactaatgca ctctctaaaa
    27121 agtcccagtc ttccaaacgt atcagggcgg tggcatttaa tagcatctga tcatcaactt
    27181 caccgtggct gataagctca gacaaaatca tatcagcgct ggtttcatac gcagctgaat
    27241 ctgctttcgg attatttttc cgatgcgata aagcacgttg ccaaattttt tgtttcatcg
    27301 acgtgctcag atactgattt ttcgccagat tactccaccc ctcaggatcg ttgaaacgtg
    27361 tcaataaccg ttgtagagca gcgtcagagt gaattacctg catatcccga cagcaaaagt
    27421 aggcagggga ttttttctcc atccttttaa caaaattaaa ctgtaagtta tctggcaatg
    27481 ctaaaaataa cacacgtatt agatcatcgt cattctcgaa tttttggtat aaataggctg
    27541 caactttgtc actctcttgt ggcgtcatcc gcaataattg accatggtgt ttcatctctg
    27601 ccagcttgaa cgctaagctt gtgacaacat tgtttgatga atcgttcgcc aactgcattg
    27661 cctgattgta ggtgagttgt cctccagcac cgacctgacg aatagaggaa tcatcactgc
    27721 ggatatagcc atcaataatg gattcaggca ataaccacgc atctttataa agctcctgaa
    27781 tgatcatatc ccaatctctt ttcgccagag tcattacgat atccagaggg agattttcac
    27841 gtagcgcaag attcaacgga ccgacaccat caggtagacg actcattcgc gtcattattt
    27901 cgacagagag cggttcacgg tcgagcaacc ccagaacaac aggctggtaa cccaattgct
    27961 gatcggtcaa ctccagaatc tcattgtcag gcatatatct cgccatactt tcaatcgttt
    28021 gttcatcatt gctattaata agttgtttgc gtaggttagc tgaaattcct ttaagccttg
    28081 ggagagttcg cattatggca agatcaaatg tcgcaatatc tcgtccttca aatgcagcaa
    28141 cgttgccttc atccagcatt tgcaattcct gcactaactc aggagaaggc ttagggcgaa
    28201 acagcgcttc ttttttcatc tgattacaga cattccctgc catgcgaata ttgcttcgct
    28261 taaaaatttc tttcagagca tcttcatcat ttattagacc ccaataatat gcatcaggat
    28321 ttattatcat cagcgctttt ttgacatttt cagtgtcatt tttgtcactc gcacggacca
    28381 tgaattcact atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg ataaatattc
    28441 cacgcgcatt aatactcccc ttaatcgaag acggattatc gtaatatgag tcgataaata
    28501 ccagtccact tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta actatattcg
    28561 ttgccgtcac gttaccgaga ataacccatg gattagaaag agagaaagtg gaaatattac
    28621 ctttgactgt caaattacca agaacaatca atttttgatt actctctata ttaccttcta
    28681 ccactaaatc gccacgatgc actttccagt ccgatgcaga taaaccgaag gggacaagat
    28741 tcacgctact gttgatagct tgttcaactt gttgtttatc ttcagcggta gccgcagaaa
    28801 aaaatggttc aatatcaact gtatttgcaa cacatcgaag ttccgcctgt actggaagac
    28861 tcagcataaa caggggaagt aaacctatcc agccgcaaat cctggactta aacatatgca
    28921 ctccatgcca ttaaaaaaga aacaaaaggg aaatgcatct ttcccttttg tatattcgtt
    28981 attctcgcga aataccatcc aacgtagaca acacttcctc agaaagatgc aatttttcag
    29041 cagccatatt ctcccgtaaa tgcgcaacag aagacgtccc tgggatcagc aaaatattcg
    29101 gtgaacgttg taacagccac gccagcgcca cctgcatggg tgttgcaccc aggctcgcag
    29161 caacatccga cagcgtagag gattgcagcg gtgtaaagcc cccgagcggg aagaacggca
    29221 cgtaggcaat gccatcgtgg gccaaagcat caatcattgc atcatcagca cggtgcgcga
    29281 tgttgtattc gttttgcaca cagacaattt cggcaatctt gcgcgcctct gcaacctgcg
    29341 tcggtgtgac gttgctcagg ccaatatgtt ttaccaggcc ttgttgttgc atctctgcca
    29401 gcacggtcag gctggcctca atcgatcctt ccgctgggcc atgaccatct cccatcataa
    29461 cgcgcaggtt aaccacatcc agtacgtcca ggccgagatt acgtagatta tcgtgcaccg
    29521 ctttttgcaa ctctgccgga gaaaaggcgg gcaaccagga agcgtcctct ccacgccgcg
    29581 caccaatttt agtgacaatt gtcaggtcgt cagagtaagg ataaagcgct tcgcggataa
    29641 tctgattggt gacgtgcgga ccataaaagt cgctggtatc aatgtgattg acgcccaatg
    29701 ccagcgcctc gcgcagcaca gttatagcga cgtggcgatc tcttggggga ccaaaaaccc
    29761 caggacctgc cagttgcatc gcgccataac caagacggtt aacggatttt gtaccgagag
    29821 taaatgtatt gctgctcatc gcaccctgcc ctgtaataaa acggattaag aaaaatcagt
    29881 atagaaacga aaagagataa ataatgcgag ggcgtaaaca agattactgg atgagttgct
    29941 ctattgtcct gaaaaacaca aaacaaaacg cgtaaagcgc cgcatccggc aataaaaaaa
    30001 ccgcaacctg tatcagcgtt gcggtttagg tatcacatgc taaaaattag aatgttcagt
    30061 atctggacgt tactgtttcg gtcgcagatt ctcatcaaat accagacgtt tcctgttcgg
    30121 ttcaacttcc cgcaatggct caacttgatg caacgtctgc ttacaacgag tcaccagcac
    30181 ctgatactca tgcgtaccct gctttttcca tgccagctcc tgctcactga gttcacgaat
    30241 cgctttcgcc gggctgccga caatcaggta attggcaggc atttctgctt tggctttcac
    30301 aaatgcggat gcgccaacaa tgctgttctc gccaatcacc gcgccgtcca tcactaccgc
    30361 gttcattccc actaatgcat tgcggcggat aatgcaacca tgaaggatag cgctatgacc
    30421 aatatgtcca tcttcttcca cgacagtatc ctgctcggga aaaccgtgca taacgcaatt
    30481 atcctgaatg ttcgcgccat ctttcaccac gatacgacca aaatcgccac gcaggctggc
    30541 atttggccca acgtaaacgc ccttgcctag aataacatcg ccgatcaata ccgctgtcgg
    30601 atggacaaaa ctctcttctg gcacaaccgg agtcagacca tctatctggt aaattggcat
    30661 atcgcctcct gttcaatatt caaaccgcca aaacgttgaa aatacaggct tcccggcgca
    30721 ggcagttcac cgaccgaggt ttcacctttt tcactaacga acgctaaagc agcaggcgct
    30781 acgcgctgat aaatgttgat acacagctga cgcgccgtat gccctgccca gtgcgccgga
    30841 agcaactcct ctggcaacaa tgggtcttta aggacgacac ggcgataaaa atggatcagt
    30901 aaaagctgaa tatgaaatgc acgctccggg gttaactcgt ctgctgcctc ttttaacagc
    30961 ggcaccagcg ggcggaatga ctgaataaag gtttcgtaca tggcattttg ttcagttaaa
    31021 tgccagcact cttccactct ggcacgcagt gctgcacgag aaagcgccag tggtatttgc
    31081 gcttcaaaac aaatcacgtt atccgccacc cccgcttcat gcaaaagtgt ctgtacatcc
    31141 gccagttttt gcgacggcga tgccatcagg ctgggtgcca gtgcgccaaa accttgccag
    31201 atcaactgct ttttgacatc agccagcgtt gatttatcta aaccttccga gagcaacagg
    31261 agccatttac catcccatgc aggttgctct gcgcgataaa ttttactttc tgcccgtcgc
    31321 gtcaggcgca agcctttatc actgaggcta tagaaactgc gtcgcccgat gcgggaaaca
    31381 tccagccagc cttctttatt aagacgaaac aaagcggtgc gcacgaaacg ctcaccaaat
    31441 cccagccctt ccagcaaagc agccagacta cccagccaga tttcaccacc acgatgggaa
    31501 agcgaatcac catacagaga ggagatcaaa gatgtgccac tgaccggaac agcgtttaca
    31561 gcatgttgga taaaagtaac aagtttactc attcgattca gtctgttgtt attgttgtga
    31621 tttatgaatc atagcacagg tcgtatcgac ttctggccct ggcgctttac ctcaccaggg
    31681 ccagacgaca cgtcaggcac caacaatatt gcgcagatca aacacccgac acgccttgcc
    31741 ttctgaacgc gggatactgc cacagttaac gatcatcaca tcggtagaga tccccaccat
    31801 cgacttaatc cgatggcgca gctggtggca cacctggcaa cgctgctcat gcgtcaatgt
    31861 caggctgctt tctttcaact ccactttcac agaaagtgaa tcaagatgcc cccggcggtt
    31921 cacctccagt tggtaatggg gcgataaatg ttcgaatttg acaatctctt cttccagttg
    31981 tgacgggaag acatttacac cgcgaatgat caacatgtca tcgctgcgtc cgctgatgcg
    32041 atccatgcgg cgcatagtcc gcgccgttcc tggtaacagt cgggttaaat cacgcgtgcg
    32101 gtaacgaatg accggcaatg cttctttggt cagcgtggtg aataacagtt cgccatgttc
    32161 accatcggca agcggtgtgc cgtcatgagg attaacaatt tcaggataga aatgatcttc
    32221 ccaaatggtt gggccgtcgg tagtttccag acactccatc gccacccccg gccccatcac
    32281 ttctgacagg ccataaatat ccagtgcggt gatccccaga cgccgttcaa tctctttacg
    32341 catggcctgt gtccacggct cagcaccaaa cactccaacc cgcagcgagc aaccgctggc
    32401 atcaccgccc aactgccgct ccagctcttc aatcaggtta aggcaataag atggcgtaac
    32461 catgatcata tctggttgaa aatcgcggat cagttgcgct tgtttttccg tctgcccacc
    32521 agacatcggg atcaccgtag cgcccagacg ttcggcacca tagtgcgcgc ccagcccacc
    32581 agtaaacagg ccgtaaccat aggcaacatg aattttgtct ttgggcgtgc cacctgccgc
    32641 acgcaaggaa cgcgctacaa tattggccca gttatcaata tcgttttgcg tatagccgac
    32701 aactgtcggt ttacctgtgg ttcccgaaga agcatgaatg cgcaccactt gttccatcgg
    32761 cacggcaaag gtgtcgaagg gatagttgtc gcgcagatcc tgtttggtgg tacagggaaa
    32821 tttacgcaga tctgaaagtt ccctgaaatc atcaggatgt acgcctgctg cgtcaaattt
    32881 gcgccgatac atcgggacat tttcatacgc gtgtttgagc gtccatttca gacgttgtgt
    32941 ttgcaacgcc tgtaactcat caacagacgc ggtttcaatc gggtcaagct ttgtattggt
    33001 tatcattgta gggtactcgc aggttgatat gctcaaacac gctccagaat catggcgatg
    33061 ccctgaccga caccgatgca catggtgcac aatgcgtaac gaccgttacg ccgatacagc
    33121 tcatggctgg cagccagtgc caggcgggca ccactcattc ccaacggatg gcctaaggcg
    33181 atagcgcctc cgttggggtt aacatgtggg gcatcatcag gcagccccaa ttcgcgtagt
    33241 acacccaacg cctgggccgc gaacgcttcg ttcagttcaa tcacgtccat atcgtgaata
    33301 ctcagccccg cgcgttccag cacccggcga gttgcaggca ccggaccaag ccccatcagg
    33361 cgcggttcca ccccggcggt tgccatggct acgattcgcg cccgcggtgt cagtccttgc
    33421 gctgctgcca tctgttcact ggcaataatc aacgcagcgg ctccgtcatt caccccggag
    33481 gcattgcctg cggtaatcac cccattggca cgaaatggtg cttttaaccc acgtaactgt
    33541 tccagcgtcg tttccgggcg cagatgctca tcatgttgta tttctgttac aacgcctttc
    33601 ttgtttttca acacaaccgg aacaatctcc tcagccagaa tgcctgagga ttgcgctttt
    33661 gccgtacgtt gctgactgcg tagcgcaaaa ctatcttgat cttctcgcga tatttttaac
    33721 agttcagcta cattctctgc cgtttccggc atgctgtcag ttccaaattg ttgagccatg
    33781 agcgggttca caaatcgcca gccaatagtg gtatcaaaca tctcagcctg acgagaaaat
    33841 gcactggttg ccttgcccat aacaaacggt gcccgagaca ttgactccac gccaccagcg
    33901 atcagcaaat cgccatcgcc cgctttaatc gcccgtgcgg cgaaccccag tgcgtccagc
    33961 ccggaaccac acaagcggtt aatggttgtg ccggaaacac tatgcggcag ccccgccagt
    34021 aacgtcgcca tccgggctac gttacggttg tcttctcccg cctgattagc acagccgagg
    34081 atcacatcat cgatacactc cgcatcgaga cgcgggtttc gcaccagcag ctcccgcaaa
    34141 gggatagcag ccagatcgtc agcccgaaca cctgataatg ccccaccata gcggccaatt
    34201 ggcgtacgaa ttccgtcaca gataaaggct tcacgcatca ggcttctcct gtaatggtgc
    34261 cgccgatgcg gtgagattta ccgcgaaaca gcgcaaccgt tttttgttgt tggttaacaa
    34321 tttcgatgtc gtaaacaccg gtttgcttgc cctgatgacg tacctgcgca gtagcagtta
    34381 aggtgtctcc ggcaaagcct ggacgcaaaa aatcaatcgt gcaggcagaa gcgacggctg
    34441 ccagcccctg gctattgcag gcgtaggcaa aggcagtatc agccagtgaa aatagctgcc
    34501 cgccgtggca actttgatga ccgttaagca tttgtgcagt gacggtcatg gtcactacag
    34561 caaagccttc atccattgag ataatgtcga tgccaagcgc tttggcgcag gcatcgttct
    34621 catacattgc atgggcattt tgccaggcct tatgactcat aaccgctctc cagaagcgct
    34681 cgttggcgca gcaatgaaca tgggcgatag cgttcttcgc catagtgatg ttgtagattt
    34741 tccaggagcc ttaatattcg ctgccagcca agttgcgctc cccaggcaag cgggccatat
    34801 ggataattca ccccaagacg catggcggta tcgatatcct gttcagaggc cacgcctttt
    34861 tgcagcgcat caagggcttc attgatgatc attgctaccg ttcgccaaat cagcattcct
    34921 gggtaatctg caatttgcag cactgttttg ccctgctgtt gcaggtaata aatggccttg
    34981 cgggtcgctg agtccgggtt caccgctgcc gcagcaatgg tcaccacctt gcccgccatt
    35041 ttgtcgacca ctaccaccgg gcgtgccagt cgtattgcca gcgcctgtgc cgtctcgcct
    35101 tgtgtctcaa tcaataaaac atcgtcaatt tccgtgacgc cgtcactttt cttttctact
    35161 ttcattgggc taaaactgtc gcttaccgct tccaggccaa caactgcctc acgttccgcg
    35221 cgccagtcgt acacgcccag cccacttttc ttgcccaacc gtccaccaat caccagttcc
    35281 tgttgcacca gcgaaggtaa aaaacgacgc tcctgccaga aagcgttaaa caccgaacag
    35341 gtgacagcaa aattgacgtc ctgaccaatc agatcggtta attccagcgg ccccatcggg
    35401 aaaccagcgc catcgcgaag tgcagcgtca atcacttctg gtgcagcaac ctgctcttcc
    35461 agtgcccgcc aggcctcgga ataataagga cgcgccacac ggttaacgat aaatccaggg
    35521 gtcgaatgac agcgcacagg ctgcttaccc caactcaacg ttagttcaca caactgctca
    35581 acaacttccg ctgccgttgc cagcccactg accacctcca ccaacttcat caccggtgcc
    35641 gggttaaaaa aatgcagccc cgcaacacgt tcaggatttt ttacctccgc agcaatcgcg
    35701 gttatagaga ttgacgaagt gttagtggtc aatagcgttt gtggcgggca aacttccgcc
    35761 agctgtgcaa agagcgcttt tttgacttcc agacgttcag acgccgcttc aatgaccagg
    35821 tccgcagctg ccagcgcgtg aatatcggtc accgggatca ggcgtttcaa tgtgcgttca
    35881 caggtttcag cagtcagttt tccccgcgtc acgcgtgaat ttagccgcgc gtgtatcccg
    35941 tcgattgcgc gggtcagcgc ttcagcagaa atgtcataca gtaaaacctg atgtccatga
    36001 ctggcggcaa cttcagcaat gccagccccc atggtgccgc tcccaatcac tgccacagtt
    36061 tgcacattta tcatcatgct atttccccgt gaactgcggt gagcgtttag ccaggaacgc
    36121 actgacacct tcacgataat ctgcgctccg tccggcaagt cgctgatagt cgcgttccag
    36181 atccagttgc gtatcgagcg tattggtttc agcgctattt atcgcttgct tgataagtcc
    36241 aagaccaaat gtcggttgtg tcgccagatg ccgtgccagc tgttgcgcgg tatctgccag
    36301 cgtttcatca tcaacaacct gccagatcat cccccattcg tgcgcctgtt cagcactcag
    36361 ttgattcccc agcagtgcca gccccatggc gcgcgctcgc ccagcaacgc gtggcagtaa
    36421 ccaggttcca ccgcaatcgg gtattaagcc taacttacta aacgccatga cgaattttgc
    36481 tgaacgggca gcaataacga tgtcgcaccc cagtgccagt gttgcgcctg cccctgccgc
    36541 cacgccattg actgcacaga tcaccggttt tggcagtttt gccaggcgac gtaccagtgg
    36601 gttatagaaa cgttcaactg acatccctaa atccggtgcg gggcctgtgg gatcaacgtt
    36661 acgatcgtta agatcctgac cagcacaaaa tccgcgcccg gcacctgtaa gtaacaggca
    36721 acggatagtg tcgtcgcgct cgacctgttt caggcactct gccagttgtg cgtgcatctc
    36781 atcattaaaa ctgttcaggc gttccgggcg gttgagcgtt agtgtcatca cgcccttttc
    36841 tacatgactg aggatgaatt ccatcattag cgtcctttaa agtcgggcgt gcgtttttgt
    36901 aagaaagcgg agatgccttc atgacgatct tctgttgccg ccagcaaggt gaataactgt
    36961 cgctcctggg caagtccggc ttgcaaagcc acttcctgcg actggcgcag cgcttgcttt
    37021 gccgcttgta aggccaacgg cgagtgacgt gccattttcg atgccagctg taaggcgtat
    37081 tcgagggtta aatcgctggg gaagacgtcg ctaaccagcc cggcctgctg tgcttgctgg
    37141 gcggtgatac tttctccgct cagcaccatt ttgctggcta acgatttacc gacactacgg
    37201 attaaacgtt gcgttccgcc tgcgccaggc atgatgccga gagtgatttc cggcaaccca
    37261 aaacgcgcgt tctctccggc aaccaccaca tcgcacaaca atgccagttc gcaacccgcc
    37321 ccaagcgcgt aaccattgac ggctgcgata agtggtttat taaaggcctg caatcgcgcc
    37381 catagctgcg gacgtgtatc gtttaaggtg gccgcgagat ctttttctgc catttcgttg
    37441 agatcggccc cagcggcaaa aaagcgtgca ttaccggtaa tcacacagac cgaaatgctg
    37501 gtatcggtag ccgcagcttc cagttcattt accagttgca tcagcagggc attatttagc
    37561 gcattacgtg cggcgggacg gttaagggtc agcaacaata ctcgttgctg acggctgacg
    37621 atcagttcgc tcatgccatc cccttcgcgt caaagtcaac caccacatcg ctggtcagcg
    37681 gcagtgcctg gcaactcaac acataacctg cggccagttc atccggttcc agactgtaat
    37741 tggtttccat cgccactttg ccacgcagca ctttgcattt gcaggtcgca cagacgccgc
    37801 ctttgcaggc atagggcaga tcagccccct ggcgcaatgc cgcatcgaga atgctttcat
    37861 cgtcggcatt aagcacgatt tcccgatccc gcccatcctg acgtacagtc actttttgtc
    37921 cgtcactttg cacgttaacg ctacgtttga cgcgcgtgcc aggcgtatta aaccgctcca
    37981 gatgaatggt tttatctggc attcccagtg cttttaaggc ggcttccgtc tcatccatca
    38041 tcgccgccgg accacaaata aatgcctcat cataaagacg aaaattaatg agcgaggccc
    38101 caagtgactg taatctttca ccgtcaatac gcccgtgaag cagatcgctg tcgagggttt
    38161 cctgactgaa aatgcacaac aactgtaaac gctgaggata tttgtctttc aggtctgcca
    38221 gtgcctggcg aaacatcatg ctctggctgg tacggttacc gtagatcagg gtgaactgac
    38281 tttcaggctc ggtttgtaaa gtggtggcga taatcgccag cattggcgta atacctgatc
    38341 ctgctgcaat agccagatag cggccctggc gttcggcctg gggctgatag ccgaaatgcc
    38401 cctgcggcac catgacctcc agcgtcatac cctggcggat gtgttcgcgg gcatagcggg
    38461 agaaacgtcc gccttcaatg gctttcaccg ccacactaat ttcgccaggc aggtagctgc
    38521 ggcaaatgga gtaacaacgg cgtaattctt caccatcaag actggctttt aaggtcaaat
    38581 gttgaccggg gcgaaagcga tacgcctcct gcaaaggctg gggcaccgca aaggtaatgg
    38641 tcaccgcatc acgggtttcc ggctcgactt ttgccactgt taaggaatga aacgttgtca
    38701 tggcatcctc aaatacattt gaaataatcg aaaggttcgc ggcaactatc gcagcggtac
    38761 aatgctttgc aggccgtgga accaaattca ctgataagtg tggtatggac gctggcgcag
    38821 cgcgggcaac gtacttctgg cggcaaatgg gcatggcaac tgtgtccggc gggcgggcta
    38881 atgccatact gtcgcagacg ctcacgggca tcgggggtca tccagtcggt ggtccatgcc
    38941 gggtcgagtt gcagcacaac ctgaacgggg gtaaacccgt gggttgtcat tgcctcacgt
    39001 atcgcgccaa tcaaatgttc cgttgccgga caaccggaat aagtcggcgt aaagccgatc
    39061 acccatcctt ctcccatctg tgtcacatta cgcaccatgc ctaaatcggt aatggtcagc
    39121 accgggatct ccgggtccgg gatctggctg agtagcgccc atatctcatg aacctgtggc
    39181 ggtgcaatgg tggccagacg ttgcataccc atctcctctg ttaccattgc tgaccgggca
    39241 agacacgctg gagatactgc atttctgcca gcatgggtcc aaggtgttcg gtatgcagtc
    39301 cttttttacc gccagtgcga tacgcctgct cttgcggtac gttcaatgtg gcttcgttga
    39361 ttccggcaaa aacttcggct tcccacgctg cgcgtaaagt gcgtggatca accgcaatac
    39421 cctcttcact cagtgcaata tcaatctcgt cggcatcgaa cagttcggca gtaaaacgcc
    39481 acaacttgtc gatggcctgc tgcatctttt gccctgatac gtcagtacca ttgcccagtc
    39541 gctccagcca gccacgacta aaacgcaggt gataacgcgc ttctttaatt gctttggcag
    39601 aaatcgccgc cagttgcgga tcacggcttt ccatcagacg ggtaaagagc gccacatgcc
    39661 atgcatcgat gaaatactgg cgtgcaatgg tgtcggcaaa attgccgttt ggttgttcaa
    39721 ccaacaataa gttgctgaac tggcgctcgt ctcgggtgaa ggccagggta tcttcatcac
    39781 cttctcccgc taattcagcg gcatacgata agaagttacg tgcctgacct aataaatcga
    39841 ggccaatgtt tgccagtgcg agatcgattt ccagttccgg tgcgtgaccg caccattccc
    39901 ccaaccgctg ggagagcacc agatagttat cgcccaggcg caaggtgtaa gccgttaact
    39961 gattcatttc cgacctcaca tgtgctcaat gccatcaggg atggtgtaaa acgttggatg
    40021 gcgatagacc ttgctttcag ccggatcaaa aaattcaccg cgttcttccg gttgcgaggc
    40081 aacaatttca ctcgccttca ccacccaaat tgaacatcct tcgctacgac gggtgtaagc
    40141 atcacgggca ttttccagtg ccatccgctc atcggcagca tgtaaactgc caacatggcg
    40201 gtgtgataag ccctgtttgc cacgcacgaa aacttcgtat aacggccagt aaacattact
    40261 cattttgaat ctccttatgc gaccttacgg gcaagttgtt tttgtgcatg ggccagcgct
    40321 gcttcccgta cccaggtacc ttcttcccag gctttacgtt tggcgtcgag cctctcctga
    40381 ttacaaattc cgcgaccgtt aattacttcg ttaaactcct gccagtcgat ctcaccaaag
    40441 cggtagtgac cgctttcagt gtcaaaatgc agatccgggt caggaacggt cataccgagc
    40501 atttcaacct gtggaacggt gttgtccacg aaacgctggc ggagttcgtc gttggtgaaa
    40561 cgtttgattt tccaggcgag acttctggcg ctgtttggcg agttatcatc gtttggcccg
    40621 aacatcatta aggctggcca ccagaaacgg ttaatggcgt cttgcaacat ctgcttttga
    40681 gcttcgctgc cctgcgccag tgccatgcag gcttcaaacc cctgacgctg gtgaaaactc
    40741 tcttctttgc agattttcac cattgctctg gcatacgggc cataagaagt tcggcataac
    40801 gccacctgat tgacgatcgc tgcgccatcc accagccagc cgataacgcc gatatcagcc
    40861 caactcaatg tcggataatt aaagatggag gaatatttca tccgtccgtc gagcattttt
    40921 tggtagatgt cttctctggc acagcccagt gtttcggcgg cgctatagag atacagacca
    40981 tgaccggctt catcctgcac tttggctaac agaatggctt tacgccgcaa ggttggtgcg
    41041 cgagtgatcc agttaccttc aggcaacata ccaacaatct cggagtgcgc atgctgccca
    41101 atctggcgga tcaatgtctt tcggtaagca tcgggcatcc agtcctgcgg ctcgatagcc
    41161 gtttcctggg ctatccgttg ctcaaagcgt tcttcttggg tcacgtcatc acctttacga
    41221 ttccagtttt cgtgaattaa ttaaaaacac gaatcacgtt gttttatgaa agttacacaa
    41281 tggttaacta aagtcacaaa ttttgtatac ttgaactgtg acagatttcg cactctattt
    41341 tgataactca ttatttatca gtgcattaat gtttttagta gttgatatac agatcacgca
    41401 gttaacattt cgttaaaaga tcccttgctt tttatgattc gcgatttaac tattagcaac
    41461 agaaatgtga aacatctgga gagtagcgat gcagcagtta gccagtttct tatccggtac
    41521 ctggcagtct ggccggggcc gtagccgttt gattcaccac gctattagcg gcgaggcgtt
    41581 atgggaagtg accagtgaag gtcttgatat ggcggctgcc cgccagtttg ccattgaaaa
    41641 aggtgccccc gccctccgcg cgatgacctt tatcgaacgt gcggcgatgc ttaaagcggt
    41701 cgctaaacat ctgctgagtg aaaaagagcg tttctatgct ctttctgcgc aaacaggcgc
    41761 aacgcgggca gacagttggg ttgatattga aggtggcatt gggacgttat ttacttacgc
    41821 cagcctcggt agccgggagc tgcctgacga tacgctgtgg ccggaagatg aattgatccc
    41881 cttatcgaaa gaaggtggat ttgccgcgcg ccatgtactg acctcgaagt caggcgtggc
    41941 agtgcatatt aacgccttta acttcccctg ctggggaatg ctggaaaagc tggcaccaac
    42001 gtggctgggc ggaatgccag ccatcatcaa accagctacc gcgacggccc aactgactca
    42061 ggcgatggtg aaatcaattg tcgatagtgg tcttgttccc gaaggcgcaa ttagtctgat
    42121 ctgcggtagt gctggcgact tgttggatca tctggacagc caggatgtgg tgactttcac
    42181 ggggtcagcg gcgaccggac agatgctgcg agttcagcca aatatcgtcg ccaaatctat
    42241 ccccttcact atggaagctg attccctgaa ctgctgcgta ctgggcgaag atgtcacccc
    42301 ggatcaaccg gagtttgcgc tgtttattcg tgaagttgtg cgtgagatga ccacaaaagc
    42361 cgggcaaaaa tgtacggcaa tccggcggat tattgtgccg caggcattgg ttaatgctgt
    42421 cagtgatgct ctggttgcgc gattacagaa agtcgtggtc ggtgatcctg ctcaggaagg
    42481 cgtgaaaatg ggcgcactgg taaatgctga gcagcgtgcc gatgtgcagg aaaaagtgaa
    42541 catattgctg gctgcaggat gcgagattcg cctcggtggt caggcggatt tatctgctgc
    42601 gggtgccttc ttcccgccaa ccttattgta ctgtccgcag ccggatgaaa caccggcggt
    42661 acatgcaaca gaagcctttg gccctgtcgc aacgctaatg ccagcacaaa accagcaaca
    42721 tgctctgcaa ctggcttgtg caggcggcgg tagccttgcg ggaacgctgg tgacggctga
    42781 tccgcaaatt gcgcgtcagt ttattgccga cgcggcacgt acgcatgggc gaattcagat
    42841 cctcaatgaa gagtcggcaa aagaatccac cgggcatggc tccccactgc cacaactggt
    42901 acatggtggg cctggtcgcg caggaggcgg tgaagaatta ggtggtttac gagcggtgaa
    42961 acattacatg cagcgaaccg ctgttcaggg tagtccgacg atgcttgcag ctatcagtaa
    43021 acagtgggtg cgcggtgcga aagtcgaaga agatcgtatt catccgttcc gcaaatattt
    43081 tgaggagcta caaccaggcg acagcctgtt gactccccgc cgcacaatga cagaggccga
    43141 tattgttaac tttgcttgcc tcagcggcga tcatttctat gcacatatgg ataagattgc
    43201 tgctgccgaa tctattttcg gtgagcgggt ggtgcatggg tattttgtgc tttctgcggc
    43261 tgcgggtctg tttgtcgatg ccggtgttgg tccggtcatt gctaactacg ggctggaaag
    43321 cttgcgtttt atcgaacccg taaagccagg cgataccatc caggtgcgtc tcacctgtaa
    43381 gcgcaagacg ctgaaaaaac agcgtagcgc agaagaaaaa ccaacaggtg tggtggaatg
    43441 ggcggtagag gtattcaatc agcatcaaac cccggtggcg ctgtattcaa ttctgacgct
    43501 ggtggccagg cagcacggtg attttgtcga ttaatcggtg aatgaagggc aacggccaat
    43561 agttgccctt ttatttcact aagttttgtg acgttgtcac attatgcatg atgtgtacat
    43621 ctattttcag ggcacccact gtatgaaaag ctggcacacc tgccaaacaa cctggcaggt
    43681 gcaggcaatc ccctttgcat cagtactgat aatgtgaacc tgactaaacc gcccacagag
    43741 cgcggttgct aacaagaaca caacatctga cgaggttaat aatgggaagc ccctctctgt
    43801 attctgcccg taaaacaacc ctggcgttgg cagtcgcctt aagtttcgcc tggcaagcgc
    43861 cggtatttgc ccacggtggt gaagcgcata tggtgccaat ggatgaaacg cttaaagaat
    43921 ttggtgccga tgtgcagtgg gacgactacg cccagatctt taccctgatt aaagatggcg
    43981 cgtacgtgaa agtgaagcct ggtgcgcaaa cagcaattgt taatggtcaa cctctggcac
    44041 tgcaagtgcc ggtagtgatg aaagacaata aggcctgggt ttctgacacc tttattaacg
    44101 atgttttcca gtccggtctg gatcaaacct ttcaggtaga aaagcgccct cacccactta
    44161 atgcgctaac tgcggacgaa attaaacagg ccgttgaaat tgttaaagct tccgcggact
    44221 tcaaacccaa tacccgtttt actgagatct ccctgctacc gccagataaa gaagctgtct
    44281 gggcgtttgc gctggaaaac aaaccggttg accagccacg caaagccgac gtcattatgc
    44341 tcgacggcaa acatatcatc gaagcggtgg tggatctgca aaacaacaaa ctgctctcct
    44401 ggcaacccat taaagacgcc cacggtatgg tgttgctgga tgatttcgcc agtgtgcaga
    44461 acattattaa caacagtgaa gaatttgccg ctgccgtgaa gaaacgcggt attactgatg
    44521 cgaaaaaagt gattaccacg ccgctgaccg taggttattt cgatggtaaa gatggcctga
    44581 aacaagatgc ccggttgctc aaagtcatca gctatcttga tgtcggtgat ggcaactact
    44641 gggcacatcc catcgaaaac ctggtggcgg tcgttgattt agaacagaaa aaaatcgtta
    44701 agattgaaga aggtccggta gttccggtgc caatgaccgc acgcccattt gatggccgtg
    44761 accgcgttgc tccggcagtt aagcctatgc aaatcattga gcctgaaggt aaaaattaca
    44821 ccattactgg cgatatgatt cactggcgga actgggattt tcacctcagc atgaactctc
    44881 gcgtcgggcc gatgatctcc accgtgactt ataacgacaa tggcaccaaa cgcaaagtca
    44941 tgtacgaagg ttctctcggc ggcatgattg tgccttacgg tgatcctgat attggctggt
    45001 actttaaagc gtatctggac tctggtgact acggtatggg cacgctaacc tcaccaattg
    45061 ctcgtggtaa agatgccccg tctaacgcag tgctccttaa tgaaaccatc gccgactaca
    45121 ctggcgtgcc gatggagatc cctcgcgcta tcgcggtatt tgaacgttat gccgggcctg
    45181 agtataagca tcaggaaatg ggccagccca acgtcagtac cgaacgccgg gagttagtgg
    45241 tgcgctggat cagtacagtg ggtaactatg actacatttt tgactggatc ttccatgaaa
    45301 acggcactat tggcattgat gccggtgcta cgggcatcga agcggtgaaa ggtgttaaag
    45361 cgaaaaccat gcacgatgag acggcgaaag atgacacgcg ctacggcacg cttatcgatc
    45421 acaatatcgt gggtactaca caccaacata tttataattt ccgcctcgat ctggatgtag
    45481 atggcgagaa taacagcctg gtggcgatgg acccagtggt aaaaccgaat actgccggtg
    45541 gcccacgcac cagtaccatg caagttaatc agtacaacat cggcaatgaa caggatgccg
    45601 cgcagaagtt tgatccgggc acgattcgtc tgttgagtaa cccgaacaaa gagaaccgca
    45661 tgggcaatcc agtttcctat caaattattc cttatgcagg tggtactcac ccggtagcaa
    45721 aaggtgccca gttcgcgccg gacgagtgga tctatcatcg tttaagcttt atggacaagc
    45781 agctttgggt aacgcgttat catcctggcg agcgtttccc ggaaggcaaa tatccgaacc
    45841 gttctactca tgacaccggt cttggacaat acagtaaaga taacgagtcg ctggacaaca
    45901 ccgacgccgt tgtctggatg accaccggca ccacacatgt ggcccgcgcc gaagagtggc
    45961 cgattatgcc gaccgaatgg gtacatactc tgcttaaacc atggaacttc tttgacgaaa
    46021 cgccaacgct aggggcgctg aagaaagata agtgattgtt tcagacaaaa aaacggcacc
    46081 aggtgccgtt ttttacttat gagcgaacca gattaatacc gtacacacac cgacttagtt
    46141 tcacaccaac cgtccagcca gtcggggcca aaatcacggc ccgttcctga ctgcttcatc
    46201 ccaccaaacg gtaagttagc gtcaattaag gtatggctgt ttacccacac cgtccctgcc
    46261 tgtaagcgat cgctatattc cagagcctgg gagagatttt gcgtccagac actggcagtt
    46321 aagccatatt ccgtgtcgtt tgccagttgt aacgcctctt ctccatccgc tactcgcacc
    46381 aggtttacca ccggaccaaa cacctcttca cgagttaagc gcaatttagc atcgggattt
    46441 accaccagcg ttggcgcaac ataatacccc tctccggctg gtccattcga cccgcgaatc
    46501 agctctgctt gctgtgcctg cgcatcgtcg aggaatgaac acactttgtc gcagtgcgca
    46561 cgagaaacca aagggttaat ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat
    46621 tttaccgcct gctcaaatcc actaaccagc gtgtcaaaca acggcgcttc aatataaatt
    46681 cgcgaactgg cggcgcatac ttgcccttga ttcaggaagc ttccggtcat caagccttca
    46741 ataacccatt gcggatcagc atcttttaat acaattgccg ggtttttacc gcccagttcc
    46801 agcgttacac gcgttaagtg atcagcagca gttctggcaa taccttttcc cgtcgcggtt
    46861 gaaccggtaa aactgatttt cgcaacatga ggatgtgacg tcagggccgc gccgcataca
    46921 gcacctgacc cggtgacgac attaaaaacg ccatcaggga taccagcctc gctggccagt
    46981 tccgccacgc gcaacatcgt cagtggcgtg gtttccgaag gcttaatcac gattgaacag
    47041 cctgctgcca gtgctggcat caccttccac ataccaatca tcaacggaaa gttccatggc
    47101 acaattcccg ccactacgcc aaccggctct ttacgcgtcc aggcctgata acgcgccccc
    47161 tggggtaagg gaatcgacaa gtccagcgtt ttacccgcga ttttggtcgt taacccggcg
    47221 gtataacgca tccagttcag cgtacagccc acttcaaaag cacgagaaat ggcaattgac
    47281 ttgccttgct ccagggtttc cagttgcgcc agctcctcac tgtgctgctc caccagatca
    47341 gcaaaacgta gcagaatacg ttcacgctct gcgggtaatc gcccggccca gcgacgcgag
    47401 acaaaggccc gccaggcaga catgactgcg ttatctacat ccgcttcgtt ggcatcagca
    47461 gtagacgcaa tttcttgccc ggtggccgga tcaaagatcg ccaatcgttt ttcactttgt
    47521 gcggggcctg gacgaccatc aatataaaga ccgtgttgac gatcgagaaa ctgttggacc
    47581 tggcttaata ctgctacatg cggctctgtc atcacttttc cttattattt acccagtgtg
    47641 atttcagtgt acgcagcgac aagaaaagtc acttttttcg ctgtgacatt cgctttgtca
    47701 gctgtgacaa gctccgcaaa tcgtgacaat aaccgcttat gtctctatgc ctgagaagac
    47761 ttatttccta tagtcaggct cattagcttc gtttactgca acacaaatgc aacaataaaa
    47821 atacatttca cagagcgaaa acgtgccatg aaccccgcag tggataatga gtttcagcaa
    47881 tggctttccc aaatcaatca ggtatgcgga aattttaccg gacacctgct gactgagcgt
    47941 tacacgggtg tactggatac gcattttgcc aaaggactaa agctgagtac cgtgacaacc
    48001 agcggggtga atttatcccg cacctggcag gaagtaaaag gcagcgacga tgcctggttt
    48061 tacaccgttt ttcagcttag tggtcaggca ataatggagc aggatgagcg tcaggtgcag
    48121 attggcgctg gcgatattac gttactcgat gcctcacgcc cctgttcgct ttactggcag
    48181 gagtcttcta aacagatttc attacttttg ccacgcactc tgctggaaca atattttccc
    48241 catcaaaaac ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt
    48301 catcgcctgt tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct
    48361 gcgctacagg cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa
    48421 cctcgtcgtg aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa
    48481 gagatattac gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac
    48541 cgaatgtttg ccgataaagg tttggtagtc gcgcaatata ttcgtaaccg tcgtctcgat
    48601 ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa aactggcagg catcggcttt
    48661 cattggggat tttctgacca gagtcatttt tcaacggtat ttaagcaacg ctttgggatg
    48721 acgccaggcg agtatcgacg taaattccgc taactaattg gcgttgtgtg tactgcaacg
    48781 ccaattagtt aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag
    48841 gagtttttga atattcacct tgtttcagga aaatagatca atcgctttct cctgtagcgt
    48901 tatcccgata ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg
    48961 tacatattcc cccggtttgg cccgagctac gagcttttgc ccggcgagtt tcgcgatatc
    49021 atctacagac acattatttt gctttgccag ttgttggtaa ctcgctttac gttcggcatt
    49081 aatgtcggtt actaatttct ctgtttcagc atccgttttc agcgcaacca gataaccata
    49141 aaatgtttca cctacccgcc cctgagttct ggcttcatcc agagtcaatg ccattacatt
    49201 gctgcttacc agcccaacaa gaaacgcaca aaaaagtaat gttttcttca tcatcgcctc
    49261 agaaaagatc gctacgagtt tcaagcagct cttcgacatc tttgtctgcc ttgatgatga
    49321 tctcatgctc aattttaacg ttcatattga tagtgatcgg ttccttgggc gcagcgactt
    49381 caatacgcgg cgtacaggca gccagcataa aagatgacgt caacgcagcc agtaaaattt
    49441 tcattgtttt tcctcacact cttttccttg cggacagtca tttcccggca gacgtgcgtt
    49501 ctgctccaac catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat
    49561 attctcttca tgatggtaat taaggtttac cgtaccgctt ttaccatcaa cgcgactggt
    49621 acctgtaatg ttggcctgca tggttaataa accgagatta tctaagttaa tttttgtcga
    49681 tgaccggcta atttccatat agcgcaacca gttaattgct gaacccgcag tcatattgtc
    49741 tttcaccacc gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc
    49801 cagccaacca tctttcacta tccatttttc gctgtttagc cataacggca atgcaccact
    49861 aaatgccccg gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga
    49921 gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag
    49981 tacgttaccg cctaacacat cgacgctaac atcagtcaac agcaagggtt cttcttcagt
    50041 ccacggataa cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac
    50101 ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa
    50161 acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc
    50221 gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc
    50281 aacctgagca tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg
    50341 ctggaataca gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac gaataccgtc
    50401 ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag
    50461 gaaatacgtc ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact
    50521 gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg
    50581 ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt
    50641 catcgtaccg tattgtaact ggtcgaaacc ggtggagagg tcggtcagcg taatcgtgct
    50701 gtcatgccat tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc
    50761 ttttccccag tagcgccact gccagcggcc cgcgtcaggg agaaaatcat tcgccagccc
    50821 atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa
    50881 acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc
    50941 gatgtccagc gaatcgatga ctcttccctt cgaacgtaat aacgcgcctg gctcaaaggc
    51001 cagcgttggg tcagtcagac ttccacttag ctgcgcgggt aagcgggcat ataaaatgag
    51061 gtccgcctgt ttcgcttcac cggtcagttg cagaggcagc tggctgttat ccatgcttaa
    51121 ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc gcttgcccct gggtcagcac
    51181 actcagtcgt ccgctgatca gagcgttctc aagccctgcc tgccagttgt cgactttgac
    51241 acccagtcgg ccactcaaag gaaaacctgc atacggccaa ctccagcgac catcgcttac
    51301 ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg
    51361 tgccagcaca atcaattgcc cgctattttc ctgccagtcc agctctgcat ccaccagtga
    51421 cggttcctgc ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg
    51481 cacgagcggc atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac
    51541 gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt
    51601 ttcgccctga taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg
    51661 ccattcctgc cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcagcat
    51721 ggactgccac tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt
    51781 cgccagacaa gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg
    51841 tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc
    51901 gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc
    51961 gagcggtagc cagatgcccg ccagagtggg aacccacagc ccgagcgtca tcaacagcgt
    52021 caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat
    52081 gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg
    52141 tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa
    52201 tatcgccata gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt agcttaaatg
    52261 tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac
    52321 gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac
    52381 tttctgctga cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc
    52441 gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat
    52501 atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg
    52561 ctgaaagtag tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt
    52621 atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac
    52681 ttctctctgg aaggtctgac cggatttact atgtatggca aaacggcagg cgttatcggt
    52741 accggtaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg
    52801 gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg
    52861 ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac
    52921 taccatctgt tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat
    52981 accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa
    53041 attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagacaaa
    53101 tccaacgacg tgatccagga tgacgtgttc cgtcgtctgt ctgcctgcca caacgtgctg
    53161 tttaccgggc accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg
    53221 ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct
    53281 tgccgctccc ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct
    53341 ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaactag
    53401 ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg
    53461 ttacgccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg
    53521 tgaccagcga taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca
    53581 gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag
    53641 ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca
    53701 ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg
    53761 cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa tagctgccac
    53821 agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc
    53881 caatcgccgt accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat
    53941 ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg
    54001 cctgctgaac aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa
    54061 acgctgctcg catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat
    54121 aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt
    54181 ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac
    54241 acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc
    54301 gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga
    54361 cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc
    54421 tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa
    54481 gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc
    54541 taccgtgcat ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg
    54601 tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct
    54661 gcatgtagcg gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga
    54721 cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc
    54781 gcaagacttt gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca
    54841 tttctttgat ggtttccgca cgtcccacga aatcaacaaa attgtcccgc tggccgatga
    54901 cacgattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcactcaa
    54961 cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg
    55021 cgaagccacc aacccatggt acgacgcggt ctatgaccat gttgaacagg cgatgaatga
    55081 tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc
    55141 ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga
    55201 attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc
    55261 agccaaacac ttgctacaaa ctctaccggg atccgtacgt agcgtggcgg tactggacag
    55321 aaccaaagaa cccggtgccc aggcagaacc gctctatctg gatgtgatga ccgcactggc
    55381 agaagccttt aataatggca agcgcgaaac tctcccccgt gtcattggtg ggcgctatgg
    55441 tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc
    55501 ggctaaaccg aaagcgcgct ttacggttgg tatttacgat gatgtaacca atctgtccct
    55561 gccgttgccg gaaaacaccc agccaaactc ggcgaaactt gaagccttgt tttatggcct
    55621 tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac
    55681 gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt
    55741 ttctcacctt cgagtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga
    55801 ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa
    55861 acctggcggc attttcctgc tcaacacacc gtacagcgca gatgaagtgt ggtcgcgctt
    55921 gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc
    55981 ggcgaaaatc gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc
    56041 ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc
    56101 gattgccaaa agttacagta gcaaaggcca ggatctggtg gaacgcaatt ggcaggctct
    56161 ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc
    56221 caatcgaccg ccagtggttt ccgatgccgc tcctgatttc gtgaaaaccg taaccgctgc
    56281 gatgctcgcc gggcttggtg acgccctccc cgtttcggcg ctgccgccag acggctcctg
    56341 gccgatgggc actacgcgct gggaaaaacg taatattgcc gaagagatcc ccacctggaa
    56401 agaggaactc tgtacccaat gtaaccactg cgttgccgct tgcccacact cagctattcg
    56461 cgcaaaagtg gtgccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga
    56521 tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggcac cggaagattg
    56581 caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat
    56641 taaagccatc aatatgatgt ctcgcctgga acatgtcgaa gaagagaaaa tcaattacga
    56701 tttcttcctc aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac
    56761 atcgcagctg attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac
    56821 gccgtatatt aaattgctga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac
    56881 cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa
    56941 cggtcgtggg ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg
    57001 tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc
    57061 cgataaaatc ccggcggaat tactgacggc gttgaaatca gacgctacgc cagaggttcg
    57121 tcgagaacag gtcgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact
    57181 gctgcgtgac gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg
    57241 ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa
    57301 cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac
    57361 accgctgggt gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct
    57421 tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca
    57481 gctgaaccag acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat
    57541 cattgcttat agcccgtgtg aagagcatgg ttacgatctg gcactcagcc atgaccagat
    57601 gcgccaactc acagctaccg gcttctggcc gctatatcgc ttcgatcctc gtcgtgccga
    57661 tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac
    57721 gttacttcac gagcaacgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca
    57781 gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc
    57841 cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta
    57901 aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca
    57961 atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcat
//
LOCUS       DAZFDP010000012        57976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFDP010000012 DAZFDP010000000
VERSION     DAZFDP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..976)
                     /gene="pgrR"
                     /locus_tag="ACHJ1G_000824"
     CDS             complement(77..976)
                     /gene="pgrR"
                     /locus_tag="ACHJ1G_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4762725.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1114..2034
                     /locus_tag="ACHJ1G_000825"
     CDS             1114..2034
                     /locus_tag="ACHJ1G_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4762726.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2034..2966
                     /locus_tag="ACHJ1G_000826"
     CDS             2034..2966
                     /locus_tag="ACHJ1G_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO4762727.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            2984..3622
                     /locus_tag="ACHJ1G_000827"
     CDS             2984..3622
                     /locus_tag="ACHJ1G_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4762728.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            3931..4659
                     /gene="mpaA"
                     /locus_tag="ACHJ1G_000828"
     CDS             3931..4659
                     /gene="mpaA"
                     /locus_tag="ACHJ1G_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4762729.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4634..5599)
                     /gene="ycjG"
                     /locus_tag="ACHJ1G_000829"
     CDS             complement(4634..5599)
                     /gene="ycjG"
                     /locus_tag="ACHJ1G_000829"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4762730.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            5718..6224
                     /gene="tpx"
                     /locus_tag="ACHJ1G_000830"
     CDS             5718..6224
                     /gene="tpx"
                     /locus_tag="ACHJ1G_000830"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4762731.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6268..7809)
                     /gene="tyrR"
                     /locus_tag="ACHJ1G_000831"
     CDS             complement(6268..7809)
                     /gene="tyrR"
                     /locus_tag="ACHJ1G_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4762732.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7957..9018)
                     /gene="ycjF"
                     /locus_tag="ACHJ1G_000832"
     CDS             complement(7957..9018)
                     /gene="ycjF"
                     /locus_tag="ACHJ1G_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4762733.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9015..10412)
                     /gene="ycjX"
                     /locus_tag="ACHJ1G_000833"
     CDS             complement(9015..10412)
                     /gene="ycjX"
                     /locus_tag="ACHJ1G_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4762734.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10567..11565
                     /gene="ycjW"
                     /locus_tag="ACHJ1G_000834"
     CDS             10567..11565
                     /gene="ycjW"
                     /locus_tag="ACHJ1G_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4762735.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11676..12581)
                     /gene="ompG"
                     /locus_tag="ACHJ1G_000835"
     CDS             complement(11676..12581)
                     /gene="ompG"
                     /locus_tag="ACHJ1G_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4762736.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12626..13708)
                     /locus_tag="ACHJ1G_000836"
     CDS             complement(12626..13708)
                     /locus_tag="ACHJ1G_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4762737.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13722..14381)
                     /gene="pgmB"
                     /locus_tag="ACHJ1G_000837"
     CDS             complement(13722..14381)
                     /gene="pgmB"
                     /locus_tag="ACHJ1G_000837"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4762738.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(14378..16645)
                     /gene="ycjT"
                     /locus_tag="ACHJ1G_000838"
     CDS             complement(14378..16645)
                     /gene="ycjT"
                     /locus_tag="ACHJ1G_000838"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4762739.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16642..17697)
                     /gene="ycjS"
                     /locus_tag="ACHJ1G_000839"
     CDS             complement(16642..17697)
                     /gene="ycjS"
                     /locus_tag="ACHJ1G_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4762740.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17707..18495)
                     /gene="ycjR"
                     /locus_tag="ACHJ1G_000840"
     CDS             complement(17707..18495)
                     /gene="ycjR"
                     /locus_tag="ACHJ1G_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4762741.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(18513..19565)
                     /gene="ycjQ"
                     /locus_tag="ACHJ1G_000841"
     CDS             complement(18513..19565)
                     /gene="ycjQ"
                     /locus_tag="ACHJ1G_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4762742.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19596..20438)
                     /gene="ycjP"
                     /locus_tag="ACHJ1G_000842"
     CDS             complement(19596..20438)
                     /gene="ycjP"
                     /locus_tag="ACHJ1G_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4762743.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20425..21306)
                     /gene="ycjO"
                     /locus_tag="ACHJ1G_000843"
     CDS             complement(20425..21306)
                     /gene="ycjO"
                     /locus_tag="ACHJ1G_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4762744.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21327..22619)
                     /gene="ycjN"
                     /locus_tag="ACHJ1G_000844"
     CDS             complement(21327..22619)
                     /gene="ycjN"
                     /locus_tag="ACHJ1G_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4762745.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(22633..24312)
                     /gene="ycjM"
                     /locus_tag="ACHJ1G_000845"
                     /pseudo
     CDS             complement(22633..24312)
                     /gene="ycjM"
                     /locus_tag="ACHJ1G_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(24524..24838)
                     /gene="pspE"
                     /locus_tag="ACHJ1G_000846"
     CDS             complement(24524..24838)
                     /gene="pspE"
                     /locus_tag="ACHJ1G_000846"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4762746.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(24913..25134)
                     /gene="pspD"
                     /locus_tag="ACHJ1G_000847"
     CDS             complement(24913..25134)
                     /gene="pspD"
                     /locus_tag="ACHJ1G_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4762747.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25143..25502)
                     /gene="pspC"
                     /locus_tag="ACHJ1G_000848"
     CDS             complement(25143..25502)
                     /gene="pspC"
                     /locus_tag="ACHJ1G_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4762748.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25502..25726)
                     /gene="pspB"
                     /locus_tag="ACHJ1G_000849"
     CDS             complement(25502..25726)
                     /gene="pspB"
                     /locus_tag="ACHJ1G_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4762749.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25780..26448)
                     /gene="pspA"
                     /locus_tag="ACHJ1G_000850"
     CDS             complement(25780..26448)
                     /gene="pspA"
                     /locus_tag="ACHJ1G_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4762750.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26615..27592
                     /gene="pspF"
                     /locus_tag="ACHJ1G_000851"
     CDS             26615..27592
                     /gene="pspF"
                     /locus_tag="ACHJ1G_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4762751.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27712..28976)
                     /gene="puuE"
                     /locus_tag="ACHJ1G_000852"
                     /pseudo
     CDS             complement(27712..28976)
                     /gene="puuE"
                     /locus_tag="ACHJ1G_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(29014..30294)
                     /gene="puuB"
                     /locus_tag="ACHJ1G_000853"
     CDS             complement(29014..30294)
                     /gene="puuB"
                     /locus_tag="ACHJ1G_000853"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4762752.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30296..31783)
                     /gene="puuC"
                     /locus_tag="ACHJ1G_000854"
     CDS             complement(30296..31783)
                     /gene="puuC"
                     /locus_tag="ACHJ1G_000854"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4762753.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(32058..32615)
                     /gene="puuR"
                     /locus_tag="ACHJ1G_000855"
     CDS             complement(32058..32615)
                     /gene="puuR"
                     /locus_tag="ACHJ1G_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4762754.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32642..33406)
                     /gene="puuD"
                     /locus_tag="ACHJ1G_000856"
     CDS             complement(32642..33406)
                     /gene="puuD"
                     /locus_tag="ACHJ1G_000856"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4762755.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33618..35036
                     /gene="puuA"
                     /locus_tag="ACHJ1G_000857"
     CDS             33618..35036
                     /gene="puuA"
                     /locus_tag="ACHJ1G_000857"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4762756.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35185..35349
                     /locus_tag="ACHJ1G_000858"
     CDS             35185..35349
                     /locus_tag="ACHJ1G_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762757.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            35339..36724
                     /gene="puuP"
                     /locus_tag="ACHJ1G_000859"
     CDS             35339..36724
                     /gene="puuP"
                     /locus_tag="ACHJ1G_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4762758.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36858..37103
                     /gene="ymjA"
                     /locus_tag="ACHJ1G_000860"
     CDS             36858..37103
                     /gene="ymjA"
                     /locus_tag="ACHJ1G_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO4762759.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37416..39059
                     /gene="sapA"
                     /locus_tag="ACHJ1G_000861"
     CDS             37416..39059
                     /gene="sapA"
                     /locus_tag="ACHJ1G_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4762760.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            39056..40021
                     /gene="sapB"
                     /locus_tag="ACHJ1G_000862"
     CDS             39056..40021
                     /gene="sapB"
                     /locus_tag="ACHJ1G_000862"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4762761.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            40008..40898
                     /gene="sapC"
                     /locus_tag="ACHJ1G_000863"
     CDS             40008..40898
                     /gene="sapC"
                     /locus_tag="ACHJ1G_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4762762.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40898..41890
                     /gene="sapD"
                     /locus_tag="ACHJ1G_000864"
     CDS             40898..41890
                     /gene="sapD"
                     /locus_tag="ACHJ1G_000864"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4762763.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41892..42698
                     /gene="sapF"
                     /locus_tag="ACHJ1G_000865"
     CDS             41892..42698
                     /gene="sapF"
                     /locus_tag="ACHJ1G_000865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4762764.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            42766..43119
                     /gene="ycjD"
                     /locus_tag="ACHJ1G_000866"
     CDS             42766..43119
                     /gene="ycjD"
                     /locus_tag="ACHJ1G_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO4762765.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            43487..44275
                     /gene="fabI"
                     /locus_tag="ACHJ1G_000867"
     CDS             43487..44275
                     /gene="fabI"
                     /locus_tag="ACHJ1G_000867"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4762766.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            44420..45549
                     /gene="yciW"
                     /locus_tag="ACHJ1G_000868"
                     /pseudo
     CDS             44420..45549
                     /gene="yciW"
                     /locus_tag="ACHJ1G_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            45617..47551
                     /gene="rnb"
                     /locus_tag="ACHJ1G_000869"
     CDS             45617..47551
                     /gene="rnb"
                     /locus_tag="ACHJ1G_000869"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4762767.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPSLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(47629..47733)
                     /locus_tag="ACHJ1G_000870"
     CDS             complement(47629..47733)
                     /locus_tag="ACHJ1G_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762768.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            47787..49772
                     /gene="pdeR"
                     /locus_tag="ACHJ1G_000871"
     CDS             47787..49772
                     /gene="pdeR"
                     /locus_tag="ACHJ1G_000871"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4762769.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(49754..49882)
                     /gene="ymiD"
                     /locus_tag="ACHJ1G_000872"
     CDS             complement(49754..49882)
                     /gene="ymiD"
                     /locus_tag="ACHJ1G_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO4762770.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            49920..50093
                     /gene="yciZ"
                     /locus_tag="ACHJ1G_000873"
     CDS             49920..50093
                     /gene="yciZ"
                     /locus_tag="ACHJ1G_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4762771.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            50183..50932
                     /gene="yciT"
                     /locus_tag="ACHJ1G_000874"
     CDS             50183..50932
                     /gene="yciT"
                     /locus_tag="ACHJ1G_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4762772.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            51201..51419
                     /gene="osmB"
                     /locus_tag="ACHJ1G_000875"
     CDS             51201..51419
                     /gene="osmB"
                     /locus_tag="ACHJ1G_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4762773.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(51545..51871)
                     /gene="yciH"
                     /locus_tag="ACHJ1G_000876"
     CDS             complement(51545..51871)
                     /gene="yciH"
                     /locus_tag="ACHJ1G_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4762774.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(51871..52608)
                     /gene="pyrF"
                     /locus_tag="ACHJ1G_000877"
     CDS             complement(51871..52608)
                     /gene="pyrF"
                     /locus_tag="ACHJ1G_000877"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4762775.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(52800..53969)
                     /gene="lapB"
                     /locus_tag="ACHJ1G_000878"
     CDS             complement(52800..53969)
                     /gene="lapB"
                     /locus_tag="ACHJ1G_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4762776.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(53976..54284)
                     /gene="lapA"
                     /locus_tag="ACHJ1G_000879"
     CDS             complement(53976..54284)
                     /gene="lapA"
                     /locus_tag="ACHJ1G_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4762777.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(54433..55197)
                     /gene="pgpB"
                     /locus_tag="ACHJ1G_000880"
     CDS             complement(54433..55197)
                     /gene="pgpB"
                     /locus_tag="ACHJ1G_000880"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4762778.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            55367..55957
                     /gene="ribA"
                     /locus_tag="ACHJ1G_000881"
     CDS             55367..55957
                     /gene="ribA"
                     /locus_tag="ACHJ1G_000881"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4762779.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(56021..>57976)
                     /gene="acnA"
                     /locus_tag="ACHJ1G_000882"
     CDS             complement(56021..>57976)
                     /gene="acnA"
                     /locus_tag="ACHJ1G_000882"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4762780.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        13939 a        15311 c        14409 g        14317 t
ORIGIN      
        1 aaaaaataac caaaacgttg aaaggaactg agaatacgtt aagagagaaa ttaaattaat
       61 gtatcagcaa gtagtgctaa acagcacctt tatacttcag cctatctata aataatgaga
      121 atgccgagcc agcatgtcga cggtgtggat agtacaggtg atgaccgggt aaatctggtg
      181 tgaatttatc caaaacacgt atcagttttt tttctttaat agcccgctca acctgatcat
      241 aaggtagata cgccaatcca tgcccatcaa ttgcagcatc aatgatcagg tctatcgtat
      301 tcagtaaaag ctgaccttcc atgcgaacac gaacttcacg tccaccgcgt attaatctcc
      361 agcgatttgc tgtacccgat gtgggaagat acaaattaat tgcctgatga tctattaatt
      421 gtgacactga cgttggaaca cttcggcgag aaaaataatc cggtgagcca acaatagcca
      481 ttggtatatc tggcccgatt cgaatggcga tcatatcttt atccatctcc ccacccagac
      541 ggacgcctgc atcaaaacgt tcagaaacga catcggtcaa accataatca atggtgagct
      601 gaatatcaat ttcaggatgc gatttcagga atgtgcgcat tgctggtaac aatatcgttt
      661 ttgctgcatg ttctacagta gtaatacgta ttgtcccgga tgggcggttc tgcagctcgc
      721 tcagggatgc cagggctgaa tctatgtcat gcaacatagg gccaagaaca gacaaaagat
      781 gctcgcccgc ttcagttgga acaacgctgc gcgtggttcg cgtcagaagc cgcaatccca
      841 atcgttcttc tatacgacgc actatctggc ttaaagctga ctgcgccatg ctcaggcggg
      901 ctgctgcacg agtgaagcta cgctcctctg caacgacgac aaacgccatc agatcagcga
      961 tttcttcacg cttcattcag tatctccatg caatctatta atcatttaaa tgataggtct
     1021 aatcatgatt ttgggtctaa tcaacttaat gatcaatatc tatagtgata tcaccaaccg
     1081 ttaaaacgaa ccgacaactc catgaggaac ggaatgatga ataataaagt cagcttcact
     1141 aacagcaata atccaaccat ctctttgtct gcagtgagct atttccctcc aaaatttgat
     1201 gaaacccgtc agtatcaggc aattgtggta tctcacccag gtgggggcgt taaagaacaa
     1261 acggccggaa cctatgccaa aaaactggcg gaaaagggat ttgtcactat tgcttatgac
     1321 gcatcttatc aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt
     1381 actgaagaca tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc
     1441 cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag
     1501 gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc gatattccgt
     1561 aatggttggg aaaacaatgt gaaatctatt gatgccctgc catacgttga agcgggttca
     1621 aacgccagaa ccagcgatat tagcagcgga gaatacgcca ttatgccact ggcaccaatg
     1681 aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacccct
     1741 cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt
     1801 acctatgatg cttaccatat ggcggaagtg tacctgactc agccgacgca gattgtggcg
     1861 ggcagccagg cagggagcaa atggatgagt gacgacctgt acgatcgagc atcaagccag
     1921 gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc
     1981 tatgtggctg aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac
     2041 atattcaaat ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac
     2101 caaattttga tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt
     2161 gtaaggagca gacgtctggc aatatctatg gcaaagcatt ggctgaaaaa ggctatctcg
     2221 ttctcgctta tgacgccagt ttccagggag aatctggtgg ggagcctcgc tggatagaag
     2281 atccgacaca gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat
     2341 atgttaatgc tgaacgtatt ggcgtgctgg gagtgtgtgg gggtggtggc tatgccatta
     2401 atgccgcgct gacagaaaaa cgaattaaag cagtagtgag tattacgggt gttaatatcg
     2461 ggcgtctttt tcgggaaggt ttcagcaatt acgatccgat tggcgcattg aatgcaatgg
     2521 cgtcgcaacg aaccaaagag gctcgtggtg gagaattaca gatcaacgaa ttacttcccg
     2581 ccagcctgga tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat gaagcaactg
     2641 actactataa aacaccacgc gggcaacaac cgggaggtgc gacaaaaatg ttgttctcac
     2701 atgcacagaa aacgctggcc tgggatgcct tcgcatttac tgaagtgtta ctgactcagc
     2761 cggtgatggt agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg
     2821 aagtctatgg ccgggcgatg gtttcgcagg atcgtcagtt agtttcatta cccgatttct
     2881 cacattatga actgtatgac aaaccggaag cagttcagaa ggcgctggaa aaagttattc
     2941 cttttttcaa cacccatctg ggataagttt catgagaaat attatgaaaa atgtactgat
     3001 tcttggtgct ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac
     3061 cattaaacaa actttatttg cccgtcagcc agcaaaaatc cacaaaccct atccaactaa
     3121 tagcaagatt attatgggag atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg
     3181 acaggacgtt gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt
     3241 gatcgctgcg atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggtat
     3301 ttatgatgaa gttccaggaa aatttggcga atggaataat gcagttattg gtgagcccct
     3361 aaaaccattc cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct
     3421 tcgccctgcc tggctgactg atgaagatat tattgattat gagcttacct cacgcaatga
     3481 accattcaaa ggtacgatcg tatcacgtaa gagtgtcgca gcattaatca cagatatcat
     3541 cgataaacct gagaaacaca ttggtgagaa tataggtatc aatcaacccg tgactgatgg
     3601 tgacaagcca ttattcatgt aaatcaatta catagttacc atcgtaatgt actatgtgca
     3661 gtacgattta ttaaacttat taaggtgaac tgatcaatcc gatgcgatta acatgtgtag
     3721 aaccagtaag aataaaatac gccatgtata taacattacg acttacatct aagtcagcat
     3781 aattctaata tctgcacatc aataaaaatg caaacaactt ccggcaaaaa agtgatacaa
     3841 gatcgatgac atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg
     3901 cctgtaatac ttcctgaacc agagacgatc atgaccgtaa accgcccacg cgccgaacgc
     3961 ggcgcatttc cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc
     4021 tggtttccgg ccactgcagc cagtcgcgaa agtggtttga ttctggctgg cactcacggt
     4081 gatgaaaact cttcggtcgt cacgctctcc tgtgctctgc ggactttgac accttctttg
     4141 cgccgccatc atgtagtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc
     4201 aatgctaatg gtgtggattt aaaccgaaac tttccggcgg cgaactggaa ggaaggtgaa
     4261 acagtttatc gctggaacag cgccgctgaa gaacgtgatg tcgtattgct gacaggtgac
     4321 aaacctggct ctgaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaaccc
     4381 gcctgggtgg tctcattcca cgatccgctg gcctgtattg aagatcccag acacagcgaa
     4441 ttaggcgaat ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa
     4501 acacctggct ctttcggtag ttggtgtgcg gatctcaacc tgcattgcat caccgcagag
     4561 tttccgccca tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg
     4621 ctgcgctggc atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat
     4681 ctaccgccag ccaggtcggt ccgtcaagat cgacgaaact gacctgcggc accagcggta
     4741 aagcggcgct aatggcccga gaggtacaca acatgcagcc cagcatcaga cgaaaacctt
     4801 gtgcacgcgc ttcagtcgcc agcgccagcg cttccgtcag accaccggtt ttatcaagct
     4861 taatgttaac catctcatag cgccctttca gcgccttcag attgctacgt gtatgacaac
     4921 tttcatctgc acaaatcggt aacggatgaa taaaattctc cagcgccgca tcgtcctgcg
     4981 caggaagcgg ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg
     5041 ccaacccttc tgcacgccag gattcatttg cgtcaacgat cagcgtcgca tcgggcacag
     5101 ctgtgcgaat tgccaccatc cgctcactga taagatggtt atccagcttc actttcagta
     5161 atttcgcgcc tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga
     5221 tgactaccgt ctgtgcagtg gtgactgtcc cgggaagcgt tatgccgatc aaatcagcca
     5281 gcgattgctg ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg
     5341 ctgcgccggc agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg
     5401 gcacgacgct cataatttgc gccattaccg aggcatcact ttccccataa cgcggatacg
     5461 gcgtgcattc gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg
     5521 cttcactgcg acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa
     5581 ataccttaac ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca
     5641 aatagcgttt gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt
     5701 aaacaggaaa gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg
     5761 ccaattccat cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc
     5821 tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga
     5881 gtattgatac cggtgtttgc gccgcatcag tacgtaagtt taaccaactg gcaactgaga
     5941 tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct
     6001 gcggcgcaga aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc
     6061 tgcaagcctt cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg
     6121 ttgtggttat tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca
     6181 ccgagccgga ttacgaagca gctctggctg tactgaaagc ataagttaat atgcctgatg
     6241 gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata
     6301 ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg
     6361 cgtgctggga taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat
     6421 ttcgtccagc gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc
     6481 cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata
     6541 gatagcgttc tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt
     6601 caggtcagcg gccagtttcg gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa
     6661 cagctcagtt aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt
     6721 caacacgttc agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac
     6781 cagattcttc tgcgtagcgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc
     6841 aacccgacgg aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga
     6901 catttcccct atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt
     6961 cttcccttcc ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc
     7021 acagttcagc gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc
     7081 aaagagatct ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc
     7141 cagtttctgc gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact
     7201 gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga
     7261 tcgcaacatc accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac
     7321 aggcgtaatc tccatcagga aattctgccc attaataacg acatgctcgt tatgggaatc
     7381 ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt
     7441 atggttgcgc aggcgatcca atttttgcgc aaaaagctga cagctcgccg ggttcgccat
     7501 atccactttg cttttcatat cgacagagag cacaggttcg ggcaacgcct ccagtaacgc
     7561 gctcaacgcc agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac
     7621 acctgcaata cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc
     7681 aaaattgagg tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct
     7741 tagcacgagt agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc
     7801 cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt
     7861 tggatagcag gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt
     7921 cggcgtaaag atatccggtc agcacccgat attgcattat ttttcgctgg gcgttttgcc
     7981 tttttgcagc gtttctttca cctgaccgat aagctgacga cggaaatccc cgaggcgagg
     8041 tttgtcatcg tcaatccacg gcagcgggcg gcaaagctcc atagctttaa tcccgagtcg
     8101 tgccgtcaga agtccagcac caattccctg agctgcgcgg gtagacaaac gagcagcgag
     8161 atcttgcgac atccagtcca tccccacttc gcgcaccagt tcgctggctc cggcaaaagc
     8221 gatattcagc aataccagct taaacagacg caaacggctg taatacccca gttcaatgcc
     8281 atacagcgtg gcgatgcgat taattaaacg cagattgcgc caggcgataa acgccatatc
     8341 gaccaacgcc agcgggctga ccgcaatcat caacgttgat tccgccgccg aacggctgat
     8401 ttcgcgccgc gcctgggcat ctaaaactgg ctggaccaaa tgcgcataca aactgaccac
     8461 ttcacggtcg ttttgcgttt catggattga ggcataccag cgttgcagcg ctggatgaga
     8521 ctgatcaata cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc
     8581 gtggctgtgc aacaaatcgc gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca
     8641 taagcgccgc cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc
     8701 ggcacatcca cccagcgcca cccagtcctg agtttgccag gcattcattg tccactgtac
     8761 accctgcccg acaacgcttg cgccaaacag agccagcccg cccatcacca ttttgcgcca
     8821 caggctgcgt ttcggacgta acgctgcatc cattaccgct tcaacttgcc cctcttcttc
     8881 aggcgcttca tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg
     8941 ctgcgccctg aattttggat tctgatcgac ctccagcgga ccgtcgaaat caatacgtgg
     9001 ttttaacggt tcggtcatcg caatttatct cctattaaaa attcaagcgc ggcatcaaga
     9061 cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg
     9121 aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg caggaacttc accgggataa
     9181 acggtgagtg gcgcaccatc gctgagccgg ttaccgcgca atgccggaat tttctcgccg
     9241 ttgacgtcaa taatgccgct ggtggtcgcc tgcaccgagg ccagccccag gcaatccata
     9301 ctaatccctt caaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa
     9361 accatattgg cgtgctgatc gatggtcaca tggtccgctt tagtggcagc aaacaatagc
     9421 ttatcgataa ccggcgaaaa caaacgccgg aacaaggtac gctgcccgta gtggaaactt
     9481 tgcatcagct gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga
     9541 ggttgcaggc aatcaaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag
     9601 aaccccttca ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg
     9661 gtgtgcttat cggcctgcgc cagtttggac tcgccccagg catcgacatc cggccacggg
     9721 aagaattgca gcgcgggcgc acctgccata tctcccggca agacaaagcg cccaggctga
     9781 ataaagtgca gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg
     9841 tctgccagcc ggttttcgtc ggcaggcgct agcgggtcca gcccttcgca catcattcgc
     9901 catttgaccg accattcgcc gcgctgacca ttgagtaagc ccgtcatctg gcgtgaccag
     9961 cttaaatagt cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca
    10021 atctccagat acagcgtgga ggtgtccttg aagtggcgta gcagcgaatc gttcgattta
    10081 aaacgtagcg ccaggcggat ttcactgacg ccgcgcgttg gcgtcggcca ggcgggagga
    10141 tcgccatata actgcgccag tccttcatca tatgtgaagc gcggaatgcc aaagtcacgc
    10201 tgaggaatgc gttttacgcc cagcaggcgc tcttcacgca ccgcgcttaa cagcggcaag
    10261 cgtgctccgg cgtgaatatt gagcaactga ttgaccatcg cagtgataaa cgctgttttg
    10321 ccgctgcggc ttagtccggt tacggcgagg cgcagatgtc tgtcgacacc ccgattcacc
    10381 agcgcattaa gttcattttt aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag
    10441 aaaaaaatca ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactgcccg
    10501 tgttgtcatc gacgggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc
    10561 tgacctatgt cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc
    10621 gtatcacgcg tgctgaataa gcaaactaat atctccccgg aagcgcgcga aaaagtgtta
    10681 cgggccattg aagaattaca gtatcagcca aacaagctgg cccgcgcgct gacctcttcg
    10741 ggttttgatg ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg
    10801 tttttctctg aagttttgca tgccatcacc gccaaagctg aagaggaagg tttcgacgtg
    10861 atattgcaga cgtcgcacaa tccggcagaa gacttacaaa aatgtgaaag caaaattaag
    10921 cagaaaatga ttaaaggcat tattatgcta agttcgccag cggatgagtc attttttgcc
    10981 caacttgata aatacgatat tcctgtcgtg gtgattggca aagttgaagg tcaatatgcc
    11041 catgtttatt ctgtcgatac cgataatttt ggcgacagca ttgcgttgac cgatgcatta
    11101 attgaaagtg ggcatcaaaa tattgcctgc ctgcatgctc cgcttgatgt ccatgtttca
    11161 gtggatcggg taaatggtta taagcagagc ctggctgcgc ataatattgc agtgcgtgat
    11221 gaatggattg ttgacggcgg ttatacccat gaaacagcac tgcaagccgc gcgggaatta
    11281 ttaagccagt caccgttgcc tgaggcagtg tttgctactg acagcctgaa attaatgagt
    11341 atttatcgtg cggcagcaga gaaaaatatt gctattccgc agcagttagc ggtggtgggt
    11401 tatagtaatg aaacgctgtc gtttatttta acgcctgcgc caggcggtat tgatatccca
    11461 acacaggagt taggtcaaca aagctgtgag ttattattcc gcttaatttc tggaaaaccg
    11521 tcaccacaga atattaccgt tgccacgcat atgacgctga aataaatacc agctaccttg
    11581 cctgaatgcc ggatgcgatg ctagcgcatc ttatccggcc tacatttata gttcggataa
    11641 agtactcgtg ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca
    11701 cctgcataat ggaatttatc gctgtcgcct tcgtcgtgat cctgccactc aaacgcgtat
    11761 tccagcgaaa cggaaagccc gttctggaaa tcataaccgt aaaataaacc tacacggtta
    11821 aaatcatggc cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc
    11881 ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga
    11941 atttcttgcg tagaaaactc accgttattg cggctgtcgt ccatattaaa gccgcgctcg
    12001 agataatagt tcactcgcaa ggcaaccgtt tcgttgaagg tatattgcag gcctgtttcc
    12061 gtttcgactc gggtatcagc gtaaccggtc gtgttcagat cgttggcaaa tttatacatc
    12121 gacaaccaac cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcgatt
    12181 ttccagcgct gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa
    12241 ttacggaaac cgccggtcag gccgaaactg aaatcatcgt tttcgaggaa ctgataatgc
    12301 acctccagct ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc
    12361 ccttcctgat aataggccag agcaattctc cacggcccgt tggcggcatt aaaatagact
    12421 gaaggctccg ccagcccatc catatcttcg ccataaccct cgacgttttc tatttcgtac
    12481 atcgcgccga tattaaagtg ccagtcgttc ctttcctcgg cctgtgcgca ggccattccc
    12541 gcacacatca ccagtgcggt acagggtaat aactttttca ttattgttcc ttgtattatt
    12601 ctgggggaat gccttccccc tgtatttagc gaattgctat ttccgtttct gcatcaaaga
    12661 aatgacattt cgtcatatca aaatgaatag tgatattttc tcctgcatga taatcattta
    12721 acgcaccagc acggaccact aactcgtgcc ccccaaccgt ggtgtagagc ataaattccg
    12781 caccggttaa ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg
    12841 gatgaatatc ttccggacgt attcccatca cgatgggctt atgcaaactt ccctgtgttt
    12901 tcagaaccgc taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat
    12961 cgccatcgat cgtgccacga ataaaattca tcgccggtga tccaataaat ccggcaacaa
    13021 acatattcgc tggttggtta taaaccgttt tcggcgcacc aacttgctga acaatcccat
    13081 ccttcataat cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt
    13141 agatcatggt ggtattcagc ttctggtgta gcttgctgat ctctgcgcgc atttgcacgc
    13201 gcagcttggc atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca
    13261 caatcgcccg cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc
    13321 gtttcaggta ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca
    13381 cctctttggc gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat
    13441 gcggatacaa cgcgtagttc tggaacacca tcgctatatt gcgtgctttg gctggaacgt
    13501 cattcattcg tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa
    13561 tcatgcgcag ggtggtcgac ttaccgcagc ccgacgggcc gacaaacacg atgaactctt
    13621 tatcggcaat ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt
    13681 gaatatgttg taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag
    13741 gccgataacc gcgaccaggt cagagagtcc gttgaaggca acagtaattg cgccccggtt
    13801 aagcccgcgc cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc
    13861 tgcgcatctt caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcggcg
    13921 agaaagattt ccgggtccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg
    13981 aaaaactcgc gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacagaagcc
    14041 agcccaaccg ggatctgctg tgcacggaga tctgccagca aagagcgaat gccgggtaga
    14101 acagcgctga ccgtcaactc gcgcagtgag tggacataga gcagattttt gcgatacgcc
    14161 agttgcgccc tctcctgcga gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc
    14221 cgcagagact catcgcggct gatccctttt agggattcgt taaactgcgc atcaatgctg
    14281 atgccaattt cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg
    14341 attacaccat ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgtgtgg
    14401 tagcggtccc attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca
    14461 cggatataag ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac
    14521 gctgcgcgtc aagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact
    14581 gttgccactg ctcaggtaac gccggattga gatgcaattc accgtcacgc acgctcaccc
    14641 cggcaaaacc ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat
    14701 catcacaact atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat
    14761 aactttgggt cagcaggcca cagcgtgcgg caacaatgcc gtggattgct ttacttaatg
    14821 acgagtcgtg aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgctg
    14881 agaactgctc cggcagcatg taattgagca tcaccacatc agcttgtttg aggatttgca
    14941 tctcgttcac ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt
    15001 tgtatttcgc cagattaatc gccggcttag ccataaacga atcatcctgc ggcaaaacgc
    15061 cgtcgggctg aatttctggc agccgcagtt ctttaaggaa catttcggcg cgatggataa
    15121 acgcatcgtc gctacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat
    15181 acgccatata gctggtgaag gcgttattat tgacatgttc ggtatattcg tcaggcccaa
    15241 taacatcatg aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg
    15301 cagtttccag aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct
    15361 gccagtattg aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg
    15421 aggccacttt ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt
    15481 cttcgccgct gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg
    15541 ctttctcctg cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg
    15601 tcggatcgct aaacagatga aacggtaaca aaaatacttc tgtatcccag aaaacgtggc
    15661 ctttgtagcc ttcgccggtt aagccttttg ccgcaatgct gctgcgctcg tcgtgggcag
    15721 gcgtcatgat gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcttcgc
    15781 cgccatttac cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatactg
    15841 caagaagttg gtcgtaactc tgctgcgcgc acatttcaag ctggcgaagc gacgcgctgc
    15901 cccactcgtc taaaacggct tgcctgtcat cccgccagtc gatccacacc agtttttgca
    15961 acgtcactgt ctcgcctgca tgaagctgcg cactggtatg ttgctgcaaa cggcgctctt
    16021 tggcggtata gcattgctgc acatcaccgc tcaccataca gcaacagctg atggccacat
    16081 cactgcgtcc atcctgggtg gtgtagatcc cctgcatcag atgctgacca aacacccgca
    16141 cctgggtttc gtcgagatgt tggcgaccgt ggttggtttg cgtggcgtcg atacctgttg
    16201 aaatcagcac tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc acaatgagcg
    16261 gcagttggtc tgccgaaaca aagcgacggc tggcgatggt gtaacctgtg ccgttgctgg
    16321 tacgccagac aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg
    16381 cttcgcggga taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag
    16441 gcaggttcac cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat
    16501 acatccctcg cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc
    16561 cgttcccctg cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt
    16621 ctgataacgt tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata
    16681 caacgccgcc accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg
    16741 cttgccctgt acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc
    16801 cagttcccgt tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg
    16861 aaacagcgtc gcaccggctt tatcaccaca aaagctgacg ttcataatcg actgttcgcg
    16921 gatgttgagt gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt
    16981 gccaaataac gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccgca
    17041 gctcttttgc gtgccgatct tttgaaagct atgcgcattc acgcttttca ccgccggaaa
    17101 gcccagcaca tacatcgccg catccagcat atgaatgccg atgtcgataa gcgggccacc
    17161 accctgcagt tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc
    17221 gcgggcggtg gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg
    17281 ttgcgtatcg agcgcaaaac ggtggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg
    17341 cgcggtatcg cacatttccc gcgcctgttc tggcgtcatg gcgggcggtt tttcgcacat
    17401 cacatggcag cccgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga
    17461 gcagacgcta accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac
    17521 ggatgcatta ccgtatttat ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac
    17581 agccaccagt tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac
    17641 ctggcctgcg cctattatcg cgacccgcag cggagagctt gtcattgcac ttttcacaaa
    17701 gacctcttag caggtacgca accaggccaa cgaatcacgg tacgcctggg caggctcttc
    17761 cgcccggata cgcccttcgt acactacata gccctgatag ttatccgcgc gcagctgttc
    17821 aaacagcgcg tggaaatcca gggtgccgct gcccggctgg tagcgatggt tatccgcaat
    17881 atgcacatga ccgagcaggt cgcggttgtc atgcagcgcc tgcgccaggt tatcttcttc
    17941 gatattcatg tgatagaaat cgccgataat ctgtacatgt ttaagatcgt tttcgacgat
    18001 gtaacggcgg gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggttc
    18061 gagatacacc acggttccgg tacgcgcggc gacctgttcc agtacgtgca gggaatcact
    18121 caccattttg cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt
    18181 aaacatgccc cacgctgccg gaacgacgat acctttaccc cccacttctg ccagcgcttc
    18241 gagaatgcgt tcgatctgct ttaagccatt aagacgacgc tcttcgataa agtcgccaat
    18301 ccaaccgtca tagccaccgc aggcggtggt caccggtaaa ccggtttctt tgattgctgc
    18361 tttgacttct tcgagattgt tgaccagcag tttgccgtca atctcaaaac catcgaagcc
    18421 catctctttg atataacgaa atttctccag aatgttttcc ggaaagaatg cctgattctg
    18481 tgtgcctatt ttcatttttt cattcctttg aattaaaacg taacgcccat tttgatgctc
    18541 tgttccggat gctgatcgac atactgcatg tagctttccg ggctcttggc aaaggtcact
    18601 accgggtcga ttaaatcttc gcaattgaga taaccgttca tcagcagttc ccagcaggtt
    18661 tcttcaatac gcttacggct ccagcgcgga taatccgggt tcggttcgct gcacgcacgg
    18721 gagaaaacaa ttttggcgtt attgaaatgc gcttcgcgtc cgaggttaaa accttcagca
    18781 aatggtttgg caaacgcgac ataagagatg gtgccgccat aagcaaggcc acgcagcgcc
    18841 gattgcagcg cgtcggcgta accgctggtt tcgataatca catcggcacc ctgcttgccg
    18901 gtcagcgttt tgatctcttt tcctacgtca gtgccaatgg ggttaaggca gaaatcagca
    18961 ccgtggcgac gggcaatatc acagcgatag gcgataggat cgacgccaat caccaccgac
    19021 gcgccagcgc gtttcgccag ttggatggct atttgaccaa tcgcgccaag ccctaccacc
    19081 accacaaaat cacccacgcg cacgttggca tcgcgcacgc cgctcatggc aaactgcgcc
    19141 gggtcgtaac aaacggcgtt tttccacgag ctgccttccg gcattttgcg cagcttgtag
    19201 ttattcaccg cgttaatgat gaccgtctcg gagagcgggc cgtagccgca tacgctgtcg
    19261 cccaccgcgt agtcggtaac gtcgctgccg cactcgataa tgtcgccaac caccatgttg
    19321 ccaagctgga atttgccaaa ctcaatgccg cgcggcgcat ctgccggacg cggagtgaac
    19381 atctgccatt cgccgttaaa gtcttcatcg ataaacgggc tggcggcgcg gaagtcgacc
    19441 acttccgttc cgtgtttcgg tgcgccgaaa cgcacgcgga ttttcacttc attggctaaa
    19501 atcgcccgat cttcatactc aaccagcgca gcaacacgcg gtgctgtggc tactaacttt
    19561 ttcatttaaa actccttgta aagaaactgg ccgctttagc ccttcacgcc accggcggtc
    19621 aaaccactct taataaaacg ttccgaaagc gcatacataa tcaccaccgg caatgcggtc
    19681 accagtgagg ccgccatcat ccgtccccag atgtaatctg gcgtactgaa cagcgcgttc
    19741 aggcccaccg gtaaggtgaa attgctggcg ctggagagga aaatcgacgc aaacagatag
    19801 tcgttccacg ccaccataaa gcagtagacg aacacggata tcagcccgga catcgccaac
    19861 ggcacggtga tgcggaagat aatttgcaga cggttgaggc catccatcat cgccgcttct
    19921 tcgatctcat cggggatggt gtcgaagtag cttttcagca taaacacggc ggtgggcaga
    19981 gtttgcgtca ccatggtgat gatcagcgcc atctcggtgt cgtaaatgcc cagcgcggtg
    20041 atgattttga acagcggcac caccagcaaa atgccagaga acatatacac cgtgtaaaag
    20101 ctggcattga tggtcatccg acctttaaag cgcaggcggg aaagcgcgta agccccaaga
    20161 atgccgagga ataccgccac cacggatgaa accaccgaca ccaccagact gttacggaag
    20221 tagtcgacaa acgggaaaat catcgggtta aaaatgtcga cgtaatgctc cagcgtccac
    20281 tgctgcggca gcagcgtagg atgcagtgag atcgcctctt tcgcgccctt gaacgaggtc
    20341 atcagcatca caaaaaatgg aaacagcgtg atgatgagaa acagcgccag cccgcagtaa
    20401 aaaccgatgc gactgagtgt gcgtttattt gttgccattg aggttcaccc gtttcctggt
    20461 cagcagaatg acagcgaaaa taatgatgaa gagcaccacc gagatcgccg ccgcttttcc
    20521 taaatcatta aaggcaaagg cggttttgta gagatataca ccgagaatat cgaccttggt
    20581 cgtcagcaaa taaacatccg cgaacatgta gaacatccag atggtgcgca gggtcaccac
    20641 cgtcgccagg accggcataa tcgcgggcag cgtgacgata cgaaaccgtt gccaggcatt
    20701 agcgccatcc atctctgccg cttcgtacag cgatttgtcg atggtctgca aaatcgccag
    20761 aaacgagata aaggcatacg ggaagtagcg ccagatggcg aacagcacca ccagcacaaa
    20821 gctactgccc ggattatcga accacagcgg tgcctgctca tagagatgca gaagatcgac
    20881 gccgaggtag ttcacaatgc cgtagccgtt gttgaacatg tatttccagg caaacaccag
    20941 cgaaatggac ggcgttacgt aggagaggat caccagcgaa cgcgcggttt tgcgcagacg
    21001 gaactcgcgg ttgaaaaaca tcgccactgc cagcccgagc acggtgctgc ccgccaccac
    21061 cagcgcggta taccagaccg tcatccacag cgagtgccag aagccgggat cggagaggat
    21121 acgcacatag ttactcaccc caacaaaggt tgactcgatg ttgggattga gcggcagacg
    21181 taaaaaactg atttcgatat tcgacaccat tggccaggcc accagaccac ccagcagtaa
    21241 taagctgggc gcgagaagca gcagcgcaaa gggcatatcg gaacgacctg aaaaaagcct
    21301 gttcattgcg gattcctgtc ctgggtttag tgctgttcga tcagttcatc cagctttttc
    21361 tggctcgctt gcagcgtgcc agggagatcg gctttaccca cggtgacgtt atgcaccatg
    21421 gagctcacca cgccagaccc cgtcacatca cccatgcggg taaagttttt atcccctact
    21481 gcaccaaaga cctgaatatt tggcagttca ccgattagct gattcggtag ttcacccagc
    21541 gccttaataa cgtcgttgtc tttccaggtg gcggtagtga caaccgcttt attcaccggc
    21601 agcgcagcac ctggcgacat catcacccag tcggcaatgt tgtctgcctg ctccataaag
    21661 gtgacaaatt tttctgctgc ttccgtctct tcggtctttt gcccggcggt aatggtcagc
    21721 gaggtcaaca tgccgtagac cgcagagttt ttctcggttg gcaccacgaa accgacgttt
    21781 ttcgggtcgc cttctttaat cacagccgga aggatatagg tggagtaaat cgccatcggc
    21841 gcggtgccgt tcataaaggc gtctttcact tccatgatgt cgttagaacc cggcatagtg
    21901 ttggcagcaa ggtcgcggta ataggtcagt gcctgcatca tctctggtgt atcaagggtg
    21961 attttgcctt cggcgttaaa gacgttagcc tggttggata acgcaaactg ggagaaggat
    22021 tgttccgtca acacgctttc tgctgtaggc agcgcaatgc cgtatttttt attcgccggg
    22081 tcattcagtt tctgtgcaac gtccagcagc tgttgccagt ttttcggctc ctcaagcccc
    22141 gcttttgcca gcacatcttt gcgataccag ataccgccaa tccaggcgct gacaggaaca
    22201 ccggtccatg cgctaccatc ttcggtacgc acaatacgca gtacgccatc gtaaaacgcg
    22261 ccttcaccaa cgttgctgat gactgtggca accgctttgc gatcgataag ctgctctttg
    22321 tccatcactt tggcgtagtc atggctggtt tctatcactt ccggcagcga accgctacgt
    22381 gaaagagtaa tgactttagt gttataggca tcttcttcca cgggcacttg cttaacggtg
    22441 atgccagggt tttctttttc aaaacgggca atcaatttac tgataacggc ctggcgctcc
    22501 tgctccaccg aagaatgcat aaattcgatg gatacattcg ttttattttc ttctttacac
    22561 cctgaaatca gggcgcagga aaccagtgct gataacagca cgatttttga tttaatcatg
    22621 agatgttcct ttttatttaa tccacataac ctgccagggg cgcagcgtta tttcattacc
    22681 caatatatca acttcactaa tcagatcccg accatgcaga ttagtaatat ttacatgctg
    22741 aatattttta ctgacattaa acaatccagt cagacaatta ccatcagcgt tacttctttg
    22801 aatacgcatt acggatgaat taatcgtatc gatggtaaaa ttattatccg gatgaaactc
    22861 gttatggctg cgacgaagtg taattaaacg cgacaactca tgatataccg catgccttaa
    22921 tgtagcttca tcgttcagtt ctcgggttat ctctttactg tgatattttt tacggttaat
    22981 cgcacggtta tatccgagtt tttcgacacc tgcgtaatca ttacgcgagc caagaatact
    23041 ttgaatatat atcgctggca caccggggaa acttaacaaa atcgcatggg cgagaataaa
    23101 cctggcgcaa cgttcttcat cgctactctc acggcggctt aacgcatcca tataggtcac
    23161 atttatttca tacggactgc gtgtaccgtc gggattattt ttccagttta ctaatgcacc
    23221 ttcctgctgt aacgcctcga ccagctctaa tatttcgctt tcaggcaaca agccccgtag
    23281 cgggtttagc ccgatgccat cgtgagaggc gaggaagtta aaccaggtgg tgttgctgga
    23341 aggtagtgtc aggttttgcg cccacgcaca aagcgcctca acgttctgtt tttgcaccgc
    23401 atgcagcacc agtggcggca gcgagaactg gtacaccata tgtgcttcgt catcgcctgc
    23461 gccaaagtaa gcaatgttgt ctttatgcgg aacattggtc tcggtaatga tcactgtacc
    23521 tggcgcaacg ttatcaataa tcgaccgtaa cagtttgata atcagatgtg ttttttccag
    23581 atggatgcag cttgttcccg gctctttcca cataaagcca acggcatcca ggcggacata
    23641 ctcagcacct ttcgcaaggt aacagagtaa aacatccacc atcgccagca acacttcagg
    23701 gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat ggcgcgttga
    23761 atgatcgcgc atctggaatg gcgttaataa cggtaacgca cgcgggcgag tgacggcgct
    23821 gagatcggtt tgcgggtcaa cggcaataaa aaaatcttca aaacctggat gctgttgtaa
    23881 atagttttta aatcattcac tttttgccga catatggttg cagacaaaat caaacattaa
    23941 atgactgcat tcaccgagtt gctgaatatc ctgccactcc cccgcttcac tggcgacctg
    24001 atgataatca attaccgaaa agccatcatc agatgaccat ggataaaacg gcaacaaatg
    24061 aacatgtgaa aaaatgcttt gcagccattg atggtaaaac tgattaaatg tgggtaatgg
    24121 ttttaaatca ttgctgtgaa attgatcggc ataggtaatt aacacgacat cactttcatc
    24181 ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac gcgttacaag
    24241 tttctgtaaa tgagttgcgg taaatgttcc accgtagatt tcgtccaggt aatccgtaat
    24301 tttctgtttc atgttgcctt tccgtggtag cggtcccatg aaacgagaga ctactccgca
    24361 gaaaataaac tgtcaattga gcacaagggt tttagtcacg caggaaatca ttttttacca
    24421 gggaaaaagc gtatttgtga gctgacgcaa ataatctatg gggatgtaaa taaggtgtga
    24481 cagaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg
    24541 gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa
    24601 ggatctcttt tgcttgccct gactggcgtc cggcattgca atacactttc acggtgtcgt
    24661 ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg
    24721 ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag
    24781 cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt ttaaacatgt
    24841 aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt
    24901 gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc gacttaacag
    24961 cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc
    25021 ccagcccgct acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc
    25081 taatttgaaa ccaggcttta ccttttgccc ggcctgttgc cagcgagtat tcataacttt
    25141 cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc
    25201 atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg
    25261 ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat
    25321 gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc
    25381 accaggatac gcaccagttt tgccggtaca tcaaaatagt tggcaatccc ggcgcagacg
    25441 ccgcggacca tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc
    25501 attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac
    25561 gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg
    25621 acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa
    25681 acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt actccttaga
    25741 cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat
    25801 tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca gttcggcaaa
    25861 ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg
    25921 gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc
    25981 cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca tcaatgcctg
    26041 ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctca ccgatctctt tcttcatgcg
    26101 tgccagcgtg tcgtccacca gcgtcacttc atgttccagg ctcttaatca gatcggtcag
    26161 tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca gcagcgccag
    26221 ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag
    26281 ctgtttcttt tctgccaacg cgcgcgccga agtagaacgt acttcaacca gtgtatcttc
    26341 catctcctgg atcatcagac gaaccagttt ctgtggatct tccgctttct ctaacagagc
    26401 gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc
    26461 ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt
    26521 ttatcttttt gatttataaa gatctgattg aagaaccaac agcaacatgc caggatgagt
    26581 tagcgaatta cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact
    26641 tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga
    26701 caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagctgattg ccagccgcct
    26761 gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa
    26821 tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca
    26881 aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact
    26941 cgctacggca ccgatgatgg tgcaggagaa attattgcgc gtgattgagt acggtgaact
    27001 ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa
    27061 tgccgatctc ccggcgatgg tcaatgaagg cacttttcgc gctgacctgc tcgaccgact
    27121 ggcttttgat gttgtacaac tgccaccact gcgcgagcgc gaaagcgaca taatgttgat
    27181 ggcagaacac tttgccatcc agatgtgtcg ggaaattaag ctgcctctgt tcccggggtt
    27241 tacagagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt
    27301 gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga
    27361 catcattatt gatcccttta aacggcgtcc gcctgaagac gctatcgccg tttcagaaac
    27421 cacctcgctt ccaacactgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga
    27481 gttgctgcaa ctcagtttgc aacaagggaa atataaccag aagcgtgcgg ctgaattact
    27541 ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg
    27601 agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata atcaatacgt
    27661 cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct
    27721 cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggta tggtcagcgg
    27781 atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca gcccctgcgc
    27841 cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc
    27901 gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg ccggaacgct
    27961 ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg cgcgttcgca
    28021 gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg ccagcgggtt
    28081 cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat tcgcgttacc
    28141 gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg
    28201 cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac
    28261 ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag
    28321 ctcttttggc gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa taatggccgc
    28381 gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg
    28441 tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg
    28501 gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca tatatgtacg
    28561 accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg cgcgagcaat
    28621 tttcaccgcg ttttccaccg cttccgcacc ggtagtgaag aatgcggttt tagcctgccc
    28681 gctcactggg gcaagggcgt taattttctc cgccagcgtg acgtagcttt cgtacggcac
    28741 aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg cgaccagatc
    28801 agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtattcgtt
    28861 gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt tacacatcac
    28921 gccaaccccg cgtggggtag cagaaagacg cgctgatgga attcattgtt gctcatgttt
    28981 cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct
    29041 gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg
    29101 gtaatgcggc agattggcga aggcatcgaa acgttcggcg tcgccgcgca gcagttcggc
    29161 aatcagacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtagc cctgcatgta
    29221 atagatgttg gtatcaaggc gaccgaactg cggcatgcgc gacaaggtca gcaggaagtt
    29281 gcccgtccag cggtaatcaa ttttcacgcc cttcagctgc gggaaggttt tcagcagttt
    29341 cggcaccaca aggcgctcaa cgtcatccgg gtcgcgcgcg ccgtagacca cgccgccgcc
    29401 gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc
    29461 cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc gttcggtggt
    29521 gatcacctgc gtgccgcacg gcatgctgcg tttcgccagt tccggctcta ctttatcgcc
    29581 cagatacgca ttcccggcga caatcacata cttcgccgtc acctgacctt tggcagttcg
    29641 cacaacggct ggcgtggtgt gctggatctg cgtcacggca gaaagttcat acacgcgccc
    29701 gccgttgagg cggatggcgt ccgcttcgcc aatggcgagg tttagcggat gaatatgccc
    29761 accgctgtga tccagcagcg caccggtata gcgatcgctg gctacttcac ggcgaatggc
    29821 gttggcgtcc agcaattcca gctgtttatt gccgtagcgt tcccagttct ctttctgctc
    29881 ttcaagtgtg gcgagctgtt tatcattcat cgccacaaac aggccgccgg ggcggtagtc
    29941 gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcaccacctt cgaacatcat
    30001 gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt cgatgtcgcg
    30061 gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc cgatgcgtga
    30121 ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg aagagagccc
    30181 ggtatagccg ccgccgacca cgcaaacgtc gcaggaaatc gactcattca gcgtgtcgaa
    30241 tggcgcgtat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg
    30301 cctccaggct tatccagatg gttttcagtt cggtgaattt ttcaagggca tgcagggatt
    30361 tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt
    30421 tgtagttatt gacgaagacg gaaccagcct tcaggcggcg gctcatgcgg tgcgcgcggg
    30481 agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg gcaagctgta
    30541 gcgcctggtc ttctgatgtg aaacgcgtga ccaccagcac cggaccgaaa atctcttcgc
    30601 ggcttaagga cgcattcggg tctacatcca caaagatggt cgggccgatg gcggcagcca
    30661 gctcagcgtt acgaccatcc agcagcagtt gccctttgct ttcgccttcc cgaataaagc
    30721 tatggaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt gcgggatcaa
    30781 gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag aattcatcgg
    30841 cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc tggttgtaga
    30901 agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg gcgaaaacga
    30961 tgttggcgct tttgccgccc gcttccagcc agacgcgttt catgttgctc tcgcccgcat
    31021 ctttcagcag ctgtttcccg gtacgggtcg aaccggtaaa ggcaatggcg tcgatatcgt
    31081 tatgacgcga cagcgcctgc cccgcttcat gaccaaaacc tgtcaccacg ttcaaaacac
    31141 catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg agcggtgatt
    31201 tttcagacgg tttcaggaca acgctgttcc ctgctgccag cgccgggcca agcttccagc
    31261 aggtcagcag cagcggaaag ttccacggta cgatggcggc aatcacaccg accggttcac
    31321 gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga
    31381 tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcagactgt
    31441 gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg gcgtgggctt
    31501 ccattaaatc ggtgagttta ttcagtaccg ctttacgttt agccggagaa gagagtgacc
    31561 agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata tcgacgctct
    31621 tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt tcaaaggttt
    31681 cattttccgc cgcagcagta tattcaccgt taataaataa gcgggtttca atggcgagac
    31741 ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact cctgtttcac
    31801 gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg gccgtatgcc
    31861 cgtttatttc tggcgacatt ctttatatac acttcatcct tcaagccgcc tctgccttgg
    31921 ctgcgttcgc tcccccgaat cacttacctg agtaaactca tcgggattcg ctcacttgcc
    31981 gccttgatgc aacttgaatg attttgtgta tagattgccg ggcgcattgc gcttacccgg
    32041 cttacagaac aaaaagatta aaacgtggtg ggcgtatggg cgctgataat tcggcaaata
    32101 cctgccgacg tattactgaa actgtgcggg atgccggtat taatggcata actttgcccc
    32161 gcgacgaggt ggtaatcctg accattaatc gtcagaacaa tttcaccttc cagtactgtg
    32221 cctatttcct caccctgatg cttaattctt tccccagtgg ttgtgcccgg ctggtacgtt
    32281 tcaaagatca tcgccagcgt gcgattcggg ttaccgttat gaaccagctt cattgacaca
    32341 ccctgactgc ccatctcaat taagtcgtcc tgattaatga cgacctgcgg ctcatcaggt
    32401 ttttccggct cggaaaagaa ttccgagagt gacagaccat acaccttcag cagcttttgc
    32461 agcgtactga tggcagggct gactttatct tgttctatcg tactgatagc actgtgagtc
    32521 agcccggaga gttcggcggc acgacgttgt gaaagcccct gctgctggcg gatttccgac
    32581 aagcgttttc ctggcgccag tccctcatca ctcatatttg catttcctta aactgtagtg
    32641 gtcagagtcg ctgtttttca gcgatatggt gctgacaagc ggtgataaag ccctcgaaca
    32701 atatacgcga aagcgcgtac tcgctactgt tccattccgg gtgccactgt acgcccagcg
    32761 caaaaggatg attgatgacg ctaaccgcct caactaagcc atccggcgag cgagcttcaa
    32821 cacgcaaccg tgggctaacg accttcgccc cttgcccatg cagagagttt acccaaaagt
    32881 tgctacattc aggtaacaac gcagacagta atcccccctc ttcaacctga acttcatgcg
    32941 acggtgcata ttgctgttcc accggcagtt cgggatcttc ccgatgttcc agcaattcag
    33001 gctgttcgca cagcttgcga tgcaacgaac caccggttgc caccaccagt tcctgtaaac
    33061 cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc ggcattaatt aacgccatgc
    33121 tcagaagatc acgcccggga tcggcgtcag gctcatcgcc gttttcacca tataggtgcg
    33181 gctgcacatt gctgggacta ccaggaagat aaatgccatc gagtttcggc aaaagttgtt
    33241 caagtaatga cggttccgcc agcgcatgtg gtagcgcaat tggcaagccg cctgcgtgga
    33301 tgatggcatt caggtacttt tcttgcagag tctgggtcgc atgaccctta agcctgttcc
    33361 tgcacattac gacaccgata accggattgt tcattatatt ttccatgctc aatctcacaa
    33421 agtggactaa attatcgcca ttactgcggt ttaaatgacc tttatgttca atattttttc
    33481 aatctagcag tgggttttca tttttgcaaa ctcaatttaa catttgacaa acatttagtt
    33541 tgcatacaga ttcgaatggt ggtcattata ttttacgctt tgataacgag cggaaaacaa
    33601 accaaaggcg aagaatcatg gaaaccaata tcgttgaagt agagaacttt gttcagcagt
    33661 cagaagagag gcggggtagc gcctttacgc aggaagtgaa acgctacctg gagcgctacc
    33721 cgaatacgca atatgttgat gttttactta ctgatttgaa tggttgcttc cgtggaaaac
    33781 gtatccctgt ttcaagcctg aagaagttgg agaaagggtg ttatttcccg gcctcggtgt
    33841 ttgcaatgga tattctgggc aacgtagtag aagaagcggg tctgggtcag gaaatgggcg
    33901 agccggatcg tacttgtgtt cctgttctcg gttccttaac cccttctgcc gccgatccag
    33961 agtttatcgg tcagatgctc ctgaccatgg tcgatgaaga tggcgctccc tttgacgttg
    34021 agccgcggaa cgttcttaac cgcctgtggc agcagctacg ccagcgcgga ttgttcccgg
    34081 ttgtagcggt agagctggag ttctatttac tggatcgtca gcgcgacgct gaagggtatc
    34141 tgcaaccgcc ctgcgcgccc ggcaccgatg accgcaatac acaaagccag gtttactccg
    34201 ttgataacct caaccacttc gccgatgtgc tcaatgatat tgatgaactg gcgcagttac
    34261 agttaattcc cgccgacggt gcggtcgccg aagcctcgcc gggtcagttt gaaatcaacc
    34321 tttaccatac cgataacgtg ctggaagcct gcgatgatgc gctggcgcta aaacgcctgg
    34381 tgcgtctgat ggcagaaaag cataagatgc acgccacttt tatggcgaag ccgtatgaag
    34441 agcacgcggg cagcggaatg catatccata tcagtatgca aaataaccgt ggcgagaacg
    34501 tgctttctga cgcggaaggc gaagattcgc cgctgctgaa aaagatgctc gccgggatga
    34561 ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa cgtgaactcg tatcgccgct
    34621 tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg ccataacaac cgcaccgtcg
    34681 ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt ggaatatcgc gtggcgggtg
    34741 ccgatgccaa cccatatctg gtgatggcag cgatttttgc cggtattttg catggccttg
    34801 ataacgagct gccgttgcag gaagaagtcg aaggcaacgg gctggaacag gaaggcttac
    34861 ccttcccgat tcgccagagc gatgccctgg gtgagtttat cgagaatgat cacctgcgcc
    34921 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    34981 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    35041 ccatgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    35101 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    35161 agactgtgac gaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    35221 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    35281 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    35341 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    35401 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    35461 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccagcgt cctatttgct
    35521 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc gccagtttcc
    35581 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aatccgcacg tcggatttat
    35641 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    35701 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    35761 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    35821 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    35881 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    35941 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    36001 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    36061 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    36121 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    36181 gctgttcgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    36241 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    36301 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    36361 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    36421 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    36481 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    36541 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    36601 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    36661 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    36721 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    36781 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    36841 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    36901 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    36961 acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa gaagcccaac
    37021 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    37081 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    37141 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    37201 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    37261 gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa cgaccaacca
    37321 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    37381 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    37441 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    37501 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    37561 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    37621 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    37681 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    37741 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    37801 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    37861 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    37921 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    37981 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    38041 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    38101 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    38161 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    38221 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    38281 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    38341 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    38401 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    38461 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    38521 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    38581 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    38641 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    38701 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    38761 gtcctttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcgatc
    38821 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggcg gcgcgtattg
    38881 aagcctatga cgaagcgcag agtattctgg cgcaggaatt gcccattttg ccgctggcgt
    38941 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    39001 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    39061 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    39121 tggcttcagc ttgagctatt tcacccctca tgcgccgtta caaggcgcgt cactgtggaa
    39181 tgcctgggtg ttctggttta acggcctgat ccactgggat tttggtgttt ccagcattaa
    39241 cggtcaaccg attgccgaac agttaaaaga ggtcttcccg gcgacgatgg aactgtgcat
    39301 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    39361 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    39421 ggtgttctgg ctggcgcttc tgttgacgct gttttgttca ctcacgctag gttggttgcc
    39481 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    39541 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    39601 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    39661 gcgtatcagc actatcgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    39721 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    39781 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    39841 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    39901 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    39961 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    40021 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    40081 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg gctggctgta ctgtgtattt
    40141 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    40201 cgccgtcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    40261 gcgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    40321 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    40381 gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc ccttcgctgc
    40441 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    40501 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    40561 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    40621 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    40681 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    40741 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    40801 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    40861 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    40921 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    40981 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    41041 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    41101 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    41161 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    41221 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    41281 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    41341 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    41401 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgaccaactc
    41461 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    41521 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    41581 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    41641 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    41701 gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct atcccactgc tggaacagtt
    41761 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    41821 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    41881 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    41941 accggaccgg ctggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    42001 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacgctgg
    42061 caaaaatgct ggcgggaatg atagagccta ccagcggcga gttattgatt gacgatcatc
    42121 cactgcattt tggcgattat tccttccgca gtcagcgtat tcgcatgatt tttcaggatc
    42181 cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat tctggatttt ccactgcgcc
    42241 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    42301 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    42361 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    42421 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggagttac
    42481 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    42541 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgaacgaggc agcaccgcgg
    42601 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    42661 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    42721 ttatccacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    42781 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    42841 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    42901 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    42961 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagagctgg
    43021 accgtgctgc gtttctggca taacgagatt gattgtaatg aggaggcggt gctggagatt
    43081 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    43141 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    43201 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    43261 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    43321 ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca
    43381 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    43441 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    43501 cggtaagcgc attctggtaa ccggtgttgc cagcaaacta tccatcgcct acggtatcgc
    43561 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    43621 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    43681 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    43741 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    43801 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    43861 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    43921 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    43981 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    44041 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    44101 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
    44161 gggtaactcc gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtggt
    44221 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaattga aataatcatt
    44281 ctgttggtaa atattgggcg gcgttctgcc gcccgttatc tctgttatac ctttctgata
    44341 tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata acgatttcct
    44401 ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc actggtatca
    44461 tgaaacgcaa tccagtactg cggagtatga cgttctgcct ctggtcccgg aagccgcaaa
    44521 ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa gaaacgctgg ctcccttcct
    44581 ttcatggctg gtccctgcgc gcgttcttgc agtggaattg ttccctgacc agcttaccgt
    44641 gacccgttca cagaccttca ccgcttatga acgcttgtct acggccctga cggttgctca
    44701 ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc cgctccccgg
    44761 gcctgattcc tccagggaaa gcaatcatag gttggcacaa atcacgcaat atgcccgcca
    44821 actggctagc tcgccttcta ttatcgacaa ccgatcgcgc cagcatctga atgacgtcgg
    44881 tcttactgcc ggggactgtg tgatcattaa ccaaatcatt ggttttattg gctttcaggc
    44941 gcggaccatt gcgacatttc aggcttatct cgggcacccg gtacgctggt tacccggtct
    45001 ggaaatacaa aactacgccg acgcgtcact gtttgctgat gaatcaatac gctggcgaag
    45061 cagctatgag gtggaaaaac tacctgaaga gtacacaaaa agttcaacag cagaactttg
    45121 ccaactggcc gaaacactct ctctctccac cctatttcac tttcccttct cgaaaggttg
    45181 ttaaacagta cacgggttaa tacacagccg gataataagc ttgcggcgtt gttatgcgcg
    45241 cggataaatg gcagtcctgc ttgttttgcc gcctgtatgg attcatcaaa tgaatataaa
    45301 aaaatcagcc cccttctgcg caagggcgaa aatgaaatta accaatgggc tgaccgtcat
    45361 tctgttgagc acgctaccgt tcaggcgata caatggctga cccgagcacc cgatcgcttt
    45421 agcgccgccc agttcagccc tttactcgaa cacgaaaaat catcaacgca gattattaat
    45481 ctgctggtat ggagcgggct gtatggctgg ataaatcgct taaaaatcgc gttgggtgag
    45541 acatattaac cttgccgcgt cagacagatt cgcgtaaaac tgtcagccgc tctaatggcc
    45601 accaaaatag acaattatgt ttcaggacaa cccgctgcta gcgcagctta aacagcaact
    45661 gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc acagaaaaag gctttggctt
    45721 cctggaagtc gacgcgcaaa aaagttattt cattccgccg ccgcagatga aaaaagtcat
    45781 gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa gaacgtgaat ccgcagagcc
    45841 agaagaactg gttgaaccgt ccctgactcg tttcgtgggt aaagttcagg gcaaaaatga
    45901 ccgtctggcc atcgttcctg atcatccact cttaaaagac gccattcctt gccgcgcagc
    45961 ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg gttgccgaaa tgcgccgtca
    46021 tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca caatacatca cttttggtga
    46081 cgaccacttt gtaccgtggt gggttaccct tgcgcgccat aatctggaaa aagaagcacc
    46141 agacggcgtc gctaccgcaa tgctcgatga aggtctggtt cgtgaagatc tgaccgcgct
    46201 ggattttgtc accatcgaca gtgccagcac agaagatatg gatgacgccc ttttcgctaa
    46261 ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt gccgatccaa ccgcgtggat
    46321 tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc gcattcacca actatctgcc
    46381 tggcttcaac atccctatgc tgcctcgcga gctttctgac gatctctgct cactgcgcgc
    46441 caatgaagtc cgcccggtac tggcatgccg catgacgctc tccgctgatg gcaccattga
    46501 agataatatc gaattctttg ccgccaccat cgaatccaaa gcaaagctgg tgtatgacca
    46561 ggtttctgac tggctggaaa ataccggtga ctggcagcct gaaagtgaag caattgccga
    46621 acaagtccgt ttgctagcgc aaatttgcca acgccgcggc gagtggcgtc ataaccacgc
    46681 actggtgttt aaagatcgcc cggattaccg ctttattctc ggtgaaaaag gtgaagtgct
    46741 ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc gtcgaagaag cgatgattgc
    46801 cgctaacatt tgtgcggccc gcgtactgcg cgataagctc ggttttggca tctataacgt
    46861 gcatatgggc tttgatccgg cgaatgccga cgcgctggca gcgttgctga aaacgcacgg
    46921 tctgcatgtc gatgccgaag aagtgctcac gctggacggt ttctgcaaac tgcgtcgtga
    46981 actggacgcg caaccaactg gtttcctcga cagccgcatt cgtcgcttcc agtcatttgc
    47041 tgaaattagc actgaacccg gtcctcactt tggcctcggt ctggaagcat acgccacctg
    47101 gacttcgccg atccgtaaat atggcgacat gatcaaccac cgtctgctga aagcggttat
    47161 caaaggcgaa actgcgacgc gcccacagga tgaaatcact gtccaaatgg ccgagcgtcg
    47221 ccgtctcaac cggatggcag aacgtgatgt tggtgactgg ttatacgcac gcttcctgaa
    47281 agacaaagcc gggaccgaca cccgtttcgc agcggaaatt gtcgatatca gccgtggcgg
    47341 catgcgtgtt cgtttggttg ataacggcgc tatcgccttt attccggcac ctttcttaca
    47401 cgctatgcgc gatgaactgg tttgcagcca ggaaaacggc actgtacaaa ttaaaggtga
    47461 aacggtttac aaagtgactg acgttattga cgtcactatt gccgaagtcc gcatggaaac
    47521 ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc acggcccatt cctcatggat
    47581 gggccgttta tttccccgct ctaccttcgt tatatccaag cagatttttt aactgacttt
    47641 cgtttgaaaa ctggcgtttt tccaaaactg gattacaaat aaatatataa aaaaaacaat
    47701 gcgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact
    47761 ccgcagttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca
    47821 actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct
    47881 tttctaccac cgaaaccaca gaacctgatc gtacattgca gttatctgcc gaacaggctg
    47941 ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg
    48001 aaagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca
    48061 acgcgtctgc atggcatgac acaccggcgg ttgctcgtga tttatcacac gggctttcct
    48121 ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga
    48181 atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaaagaa cacgacgtca
    48241 ttgggcaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca
    48301 ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacat
    48361 gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa
    48421 acgagatttt tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggagc
    48481 gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc
    48541 aggatttaat cgatcacgct attaatcatg cagataacaa taaagttggg gttgtgtatc
    48601 ttgatttgga taatttcaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt
    48661 tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc
    48721 gtccaggtgg ggatgagttt ctggtactgg catccaacac ctcacaaagc gcgctggaag
    48781 caatggcatc acgaattttg acccgcttac ggctcccctt tcgcattggt ttaattgaag
    48841 tttataccag ctgttcagta ggtattgcac tctctcccga acatggttca gacagcacgg
    48901 ctattattcg tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat
    48961 tttgcgtttt taccccagaa atgaatcaac gggtatttga atatctctgg ctggatacca
    49021 acttgcgtaa agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct
    49081 ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt
    49141 tgattccacc gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag
    49201 gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa gtggcgggaa aaaggcataa
    49261 acctgcgagt ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg
    49321 ccctgaaaca ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga
    49381 cagagagttg tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc
    49441 aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac
    49501 tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca
    49561 aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc
    49621 ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg
    49681 ggatcaatga gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac
    49741 gctggtataa acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat
    49801 atcccgtcat tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct
    49861 cttttagcgg caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga
    49921 tgtctgaatt tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg
    49981 tggcaggcga ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc
    50041 gccaggttgc tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt
    50101 gccttcaggt tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta
    50161 taacgttata agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat
    50221 tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac
    50281 cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt
    50341 tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa
    50401 gcgtgaactc gccgagtttg ccgcgtctct ggttcaaccg ggcgaaacca tctttatcga
    50461 aaatggtagc agtaatgccc tgctcgcccg gactctgggc gaacagaaga aaaatgtcac
    50521 tatcatcaca gtcagcagct acattgcgca tttgctaaaa gacgcgcctt gtgaagtcat
    50581 tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca
    50641 gtgcatccag caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga
    50701 aactggattc accggtcgcg atatgatgcg taccgatgtg gtcaatgccg tgctggaaaa
    50761 agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc
    50821 catcggtccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga
    50881 tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc
    50941 cttctctacg gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga
    51001 taattaagac tatttacctg ttattaacac gcttaagata taaaattatt atcagcgata
    51061 taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa
    51121 taatttcacc aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca
    51181 taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg
    51241 caattacttt ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca
    51301 ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt
    51361 tgggtacatt aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat
    51421 cgtaaataaa tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga
    51481 aataactgaa taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt
    51541 tatgttaacc gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata
    51601 aatcacgctt atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc
    51661 attttttctt cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgacaccgg
    51721 taatcaggca aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccacac
    51781 cgtcgccttt agggcgtaca ggggccgctt tgggctcatc aatacgtccg gtttccgttg
    51841 agtagaccag acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat
    51901 cgctttcagc gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata
    51961 atcaacacca gccgacaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc
    52021 actcccctgc ggacgaatgc ccggcgtaac cagtttgaac tcctgaccga atacctgttt
    52081 aaagcgcaca gcttcctgag cagaacacac cacaccatca aggccacatt tttgcgtcag
    52141 tgccgccaga cgttctgcat aatctgcagg tgacagtgtc atgccaagat cgaccaggtc
    52201 gctggcttcc atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa
    52261 cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc ccaccagagg catgaacatt
    52321 caccatccac acgcctaagt cagctgcagc agcgacagcg tgcgcagcag tgttaggaat
    52381 atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa
    52441 ctgtggccca aacaatgtaa acatctcttt gccgaccttc agacgacaat cgcgtgggtc
    52501 gatcttgtcg acaaaggaca gcgcgtcatc acgattatga taatcaaggg caacaaccac
    52561 aggagaattc gtaacagcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc
    52621 ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgga
    52681 aataaaaaat taacagttgc gatttttccc ctggcaggct ttttctgcgc cgtcgttgcc
    52741 ggagcacaat ggaacatcaa taactagtat gttgtaacta aagtaggatt ttttaaaaat
    52801 tacaggccat caagaccgcg aatcggttta atggttgacc aggcccgaca agacggacaa
    52861 tgccagtaga gggtgtatgc ggtaaaacca catttctggc agcgataacg aggcttacta
    52921 cgtacctttt cgccaaccat gtcacgcagc accatcaggc tctctttggc acgcccttct
    52981 tccgcttcat ttaagtggta atccattaac ttatggaaca cacgcatggt cggatgacgc
    53041 tgaagctggc gcgtaatata gacctgtgcg gcctcactac cgtcgcgcgc ttcgatgata
    53101 tcagcaagca tcaattcagc atcggcaccg gtgttctctt ccaccgcgcg ttgcaggaat
    53161 tctgcccatt cggcagtttt acccaactgc tgatagcagg tttgcaacat ttccagcgtt
    53221 tcgctgacca gttctctgtc ctgggatatc acgcgttgca aactttcgac ggctttggcg
    53281 tattctcctt tcgccataaa cacgcgtccc atcatgatgg atacgcgggc gctgttttta
    53341 tctgccgccg cccctttttt tagcaaggtc atggcacgat cgagatcgtc gctggccata
    53401 tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga cgcgctgttt atctttaccc
    53461 agcttcacca ggcgttcggc aacatcaatt gctttctgcc actcactggt agcctggtag
    53521 atttgtagca actgttgcag agcgccaatg cggaagtcag tttcatcggt cagctgattg
    53581 aacatgtctt ccgcgcggtc gtataacccg gcggccatgt aatcacgccc tagttgttga
    53641 atcgccaaca gacgctgttc ataggtcagc gaggcgcttt ccattagggt ctgatggatg
    53701 cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt ttccgagcgt aaggtgggct
    53761 tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca ggtctaccgc tttatcctgt
    53821 tgattactaa gcaggaagtt aacccccgct acgtaatcac gcgacaagcg gttggcttca
    53881 tcttgcttgt tttgttgcgc acttctgcgg cccatatacc agccataggc agcggctaca
    53941 ggcaaaagca gaaacaacaa ctccagcata gaaagttatt ccttcgccgc tgacgagtgc
    54001 ggcactacag ccacgtcagt cgcgggtgaa agctggtttt ccagtcgctt tattttacgt
    54061 tcagcgcgcg ccagggaaac acgaactcgc agccagaaca ggccacaaat caaccaaccg
    54121 atagcaaacc ccgcagcaaa caataccgcc agcaatgtgg aaatacggta ctccccttgc
    54181 gctaacagat aattaaacgt cacctgttga tcgttctgcg cacccaacgt gaccgaaatc
    54241 acgaagatcg ctaacaccag taagaaaatg agtaaatatt tcacattact tcccgttatg
    54301 tggttagagc gaataaatta tggtcaactt accgcaatag ccctttaaag taccattttc
    54361 gcgccgtctg cgaaatggca tccacgcgca ttgttgatga tttaagggct aaattcggaa
    54421 aatcaaccag cgttaacttt cttgttctcg ttgcgctatt tcgcgatttt cttccgcagg
    54481 tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt gccaccgcca ccagcgccca
    54541 cgaaatcaac gtagctacta ccagatcgcg tggccaatgc atcccgagca gcaggcggct
    54601 tcccatgact cccgttgccc agaccagcaa gatagcaatg gttaacgttc gccgacgcgg
    54661 ccacagcaaa ccaacggcca gcagtgccca actggcagca aacatcgtgt gaccggaagg
    54721 aaaggcaaac cccgtctctt tctgccaatg tgaacgcaaa tattgtggga tatttttctc
    54781 ttcagccaac tgttctttca ctagatttcc gcgttctgct cgctttaaag tgtagaactc
    54841 atcaaccgga atatgatgtg ttttttccag ccagataaca aaaggtcgtg gttcctggac
    54901 tttgtctttg atccaggatt taacgccttg tcccacaagg attgcggccg ccagaatggc
    54961 aaataataca aaggcagcct taatgcgaaa acgcagacac cagagaaacc agccgaataa
    55021 aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt tcagtaaccc aaaaagccgc
    55081 ttttagtagc caactttgtt ctccaggttg ccaacgccag ccagaaatcc atacggctac
    55141 tggcatgaca agcaatagtg cagctcccac tgcggtacgt ctggcaatcg aacgcatggc
    55201 ctctcctttt tgataagtcc cacaatcata actgaaaacg ccagttccag gaaaaattga
    55261 cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat taggtgaacc ccttctcgtt
    55321 atggcaaaat aagccaatac agaaccagca ttatctggag aatttcatgc agcttaaacg
    55381 tgtggcagaa gccaaactgc caaccccatg gggcgatttc ctgatggtgg gatttgaaga
    55441 actggcaacc ggacacgatc atgtcgcgct agtctatggc gatatttccg ggcatacccc
    55501 ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac gccctgttca gcttgcgctg
    55561 cgattgtggc ttccagctcg aagcggcatt gacgcaaatt gccgaggaag gccgtggtat
    55621 tttgctgtat caccgtcagg aaggtcgtaa cattggtctg ctgaataaaa tccgcgctta
    55681 cgcactgcag gatcaaggtt acgataccgt agaggctaac caccagttag gcttcgccgc
    55741 tgatgagcgc gacttcactc tttgcgctga tatgttcaaa ctccttggcg tcaatgaagt
    55801 ccgcttgtta accaataacc cgaaaaaagt cgaaattctg accgaagcag ggattaatat
    55861 tgttgaacgc gtaccattga ttgtaggtcg taaccccaat aacgaacatt atctcgatac
    55921 caaagccgag aaaatgggcc atttgctgaa caaataaccc tcttgcattg tgtaattaat
    55981 ttgcttgccg gaagcaaaat aaccggcaag caaatacttg ttacttcaac atattacgaa
    56041 tgacataatg caaaatgccg tcgttctggt agtaggtcaa ctccgtcgcg gtgtcgatac
    56101 gacaacggca gggtacgacc tcctggctac catccgcgcg cgtaagcgtc accggaaccg
    56161 tcgcaccggg ttgtagattt tgcagatcgc caatatcaat cttctcttcc ccggttagtc
    56221 ctaacgtttt acgcgttacg ccttgcggaa attccagcgg caggatgccc atgccaatta
    56281 aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac cacacgaatt ccaagcagac
    56341 gaggaccttt tgccgcccag tcacggcttg agcctgatcc atactctttc ccggcaatca
    56401 ccgccagcgg cgtttgttcc ttcttatagc gcatcgcagc atcataaata gagactacgt
    56461 cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc aggcaccatt tcattacgga
    56521 tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg gttaccacgc cgcgaaccgt
    56581 aggagttaaa gtcttttcgc tcaacacccc gaccttgtag atatcgaccc gctgggctgt
    56641 cgggcttaat actgcccgcc ggagagatat ggtcagtggt gactgaatcc cccagcattg
    56701 cgaggatccg cgcaccgtga atatcttcca ctggtgctgg tgttgcctgc atttcatcaa
    56761 agaaaggcga taagcgaata taggttgagt cctcctgcca accgtaggta tcggatcgtg
    56821 tgacgttaat ttccttccac tctgctgtgc cttcaaaaac ttctgcgtac tctttgcgga
    56881 acatttctgt ggagacttgt tctaccgcac gggcaatttc ttgtgccgat ggccagatat
    56941 ctttcagata aaccgggtcg cctttacgat catgaccaat aggctcagca gccaggttga
    57001 tattcatatt tcccgccagc gcataggcaa ccaccagcgg cggcgaggcc agccagttag
    57061 ttttaaccag cggatggata cggccttcaa agttacggtt gccggacagc accgcaccga
    57121 cggttaaatc gccttttttg attgccgttt cgataggatc gggcagcggc ccagagttac
    57181 caatacaggt ggtacaaccg tatcccacaa ggttaaaccc cagttcgtcg agatacggtg
    57241 tcagtttcgc ttttgccaga taatcagaaa cgactttcga acccggtgcc agcgacgctt
    57301 tgacccacgg ttgccgcttg aggcccagag ttacggcttt tttcgccagc aagcctgcgg
    57361 ccatcagcac acttgggtta gaggtgttgg tgcacgaggt tatcgcagca atgaccacag
    57421 cgccatcagg taactgatac tgatgtccgt tcataacata atcgaccggc tggcgatctt
    57481 tatgcgtggc attcacttcc agttcgttac tggcggcaaa tgcttttggt acatcgggca
    57541 gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct cgcttcaacg tcattcatat
    57601 ccagttctaa cgtactggta aaaatcggtt catcgcccgg gttacgccac atgccctgcg
    57661 ctttggcata tttttcgacc aactcaacct gatcttcgct gcgcccgctt aaacgcatgt
    57721 aatcgagggt tacagcatcg attgggaaga agccacaggt ggcaccatat tctggcgaca
    57781 tattggcaat ggtggcgcga tccgccagcg gtagtgaatc cagaccatca ccataaaatt
    57841 cgacgaattt ccccaccacg ccatgtttgc gcagcatttg ggtaacagtg agaaccaggt
    57901 ctgtggcggt aataccttca cgtaatttac cggtaagttt aaagcccact acgtccggga
    57961 taagcatgga aaccgg
//
LOCUS       DAZFDP010000013        56246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFDP010000013 DAZFDP010000000
VERSION     DAZFDP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..1146
                     /gene="phoE"
                     /locus_tag="ACHJ1G_000883"
     CDS             91..1146
                     /gene="phoE"
                     /locus_tag="ACHJ1G_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4762781.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1185..1586)
                     /gene="crl"
                     /locus_tag="ACHJ1G_000884"
     CDS             complement(1185..1586)
                     /gene="crl"
                     /locus_tag="ACHJ1G_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4762782.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1644..2888)
                     /gene="frsA"
                     /locus_tag="ACHJ1G_000885"
     CDS             complement(1644..2888)
                     /gene="frsA"
                     /locus_tag="ACHJ1G_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4762783.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2980..3438)
                     /gene="gpt"
                     /locus_tag="ACHJ1G_000886"
     CDS             complement(2980..3438)
                     /gene="gpt"
                     /locus_tag="ACHJ1G_000886"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4762784.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3699..5156
                     /gene="pepD"
                     /locus_tag="ACHJ1G_000887"
     CDS             3699..5156
                     /gene="pepD"
                     /locus_tag="ACHJ1G_000887"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4762785.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5213..5827)
                     /gene="prfH"
                     /locus_tag="ACHJ1G_000888"
     CDS             complement(5213..5827)
                     /gene="prfH"
                     /locus_tag="ACHJ1G_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4762786.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5824..6963)
                     /locus_tag="ACHJ1G_000889"
     CDS             complement(5824..6963)
                     /locus_tag="ACHJ1G_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4762787.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7209..7661)
                     /locus_tag="ACHJ1G_000890"
     CDS             complement(7209..7661)
                     /locus_tag="ACHJ1G_000890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4762788.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7658..8713)
                     /gene="dinB"
                     /locus_tag="ACHJ1G_000891"
     CDS             complement(7658..8713)
                     /gene="dinB"
                     /locus_tag="ACHJ1G_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4762789.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8784..9707)
                     /gene="lafU"
                     /locus_tag="ACHJ1G_000892"
     CDS             complement(8784..9707)
                     /gene="lafU"
                     /locus_tag="ACHJ1G_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4762790.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9710..10573)
                     /gene="motA"
                     /locus_tag="ACHJ1G_000893"
     CDS             complement(9710..10573)
                     /gene="motA"
                     /locus_tag="ACHJ1G_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4762791.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10586..11302)
                     /locus_tag="ACHJ1G_000894"
     CDS             complement(10586..11302)
                     /locus_tag="ACHJ1G_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO4762792.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11322..11789)
                     /locus_tag="ACHJ1G_000895"
     CDS             complement(11322..11789)
                     /locus_tag="ACHJ1G_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO4762793.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11797..12858)
                     /locus_tag="ACHJ1G_000896"
     CDS             complement(11797..12858)
                     /locus_tag="ACHJ1G_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4762794.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12855..13166)
                     /locus_tag="ACHJ1G_000897"
     CDS             complement(12855..13166)
                     /locus_tag="ACHJ1G_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO4762795.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13171..13563)
                     /gene="fliS"
                     /locus_tag="ACHJ1G_000898"
     CDS             complement(13171..13563)
                     /gene="fliS"
                     /locus_tag="ACHJ1G_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4762796.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13586..14902)
                     /gene="fliD"
                     /locus_tag="ACHJ1G_000899"
     CDS             complement(13586..14902)
                     /gene="fliD"
                     /locus_tag="ACHJ1G_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4762797.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15109..16023)
                     /gene="lafA"
                     /locus_tag="ACHJ1G_000900"
     CDS             complement(15109..16023)
                     /gene="lafA"
                     /locus_tag="ACHJ1G_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO4762798.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            16509..17351
                     /locus_tag="ACHJ1G_000901"
     CDS             16509..17351
                     /locus_tag="ACHJ1G_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO4762799.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(17406..18383)
                     /locus_tag="ACHJ1G_000902"
     CDS             complement(17406..18383)
                     /locus_tag="ACHJ1G_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO4762800.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(18400..19329)
                     /gene="flgL"
                     /locus_tag="ACHJ1G_000903"
     CDS             complement(18400..19329)
                     /gene="flgL"
                     /locus_tag="ACHJ1G_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4762801.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(19344..20720)
                     /gene="flgK"
                     /locus_tag="ACHJ1G_000904"
     CDS             complement(19344..20720)
                     /gene="flgK"
                     /locus_tag="ACHJ1G_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4762802.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGISMEEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(20902..21201)
                     /locus_tag="ACHJ1G_000905"
     CDS             complement(20902..21201)
                     /locus_tag="ACHJ1G_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO4762803.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(21201..22301)
                     /locus_tag="ACHJ1G_000906"
     CDS             complement(21201..22301)
                     /locus_tag="ACHJ1G_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4762804.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(22316..22981)
                     /gene="flgH"
                     /locus_tag="ACHJ1G_000907"
     CDS             complement(22316..22981)
                     /gene="flgH"
                     /locus_tag="ACHJ1G_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4762805.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(23064..23849)
                     /gene="flgG"
                     /locus_tag="ACHJ1G_000908"
     CDS             complement(23064..23849)
                     /gene="flgG"
                     /locus_tag="ACHJ1G_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4762806.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23928..24665)
                     /locus_tag="ACHJ1G_000909"
     CDS             complement(23928..24665)
                     /locus_tag="ACHJ1G_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO4762807.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24665..25867)
                     /gene="flgE"
                     /locus_tag="ACHJ1G_000910"
     CDS             complement(24665..25867)
                     /gene="flgE"
                     /locus_tag="ACHJ1G_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4762808.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25891..26604)
                     /locus_tag="ACHJ1G_000911"
     CDS             complement(25891..26604)
                     /locus_tag="ACHJ1G_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO4762809.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(26604..27035)
                     /gene="flgC"
                     /locus_tag="ACHJ1G_000912"
     CDS             complement(26604..27035)
                     /gene="flgC"
                     /locus_tag="ACHJ1G_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4762810.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27038..27373)
                     /locus_tag="ACHJ1G_000913"
     CDS             complement(27038..27373)
                     /locus_tag="ACHJ1G_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO4762811.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27455..28192
                     /gene="flgA"
                     /locus_tag="ACHJ1G_000914"
     CDS             27455..28192
                     /gene="flgA"
                     /locus_tag="ACHJ1G_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4762812.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28273..28551
                     /gene="flgM"
                     /locus_tag="ACHJ1G_000915"
     CDS             28273..28551
                     /gene="flgM"
                     /locus_tag="ACHJ1G_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4762813.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28564..28992
                     /gene="flgN"
                     /locus_tag="ACHJ1G_000916"
     CDS             28564..28992
                     /gene="flgN"
                     /locus_tag="ACHJ1G_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO4762814.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29119..30048
                     /locus_tag="ACHJ1G_000917"
     CDS             29119..30048
                     /locus_tag="ACHJ1G_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO4762815.1"
                     /translation="MSFIECYEPEYVRKFLARPPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            30227..32671
                     /locus_tag="ACHJ1G_000918"
     CDS             30227..32671
                     /locus_tag="ACHJ1G_000918"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4762816.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(32789..33184)
                     /locus_tag="ACHJ1G_000919"
     CDS             complement(32789..33184)
                     /locus_tag="ACHJ1G_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO4762817.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(33187..33621)
                     /gene="fliJ"
                     /locus_tag="ACHJ1G_000920"
     CDS             complement(33187..33621)
                     /gene="fliJ"
                     /locus_tag="ACHJ1G_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4762818.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YFVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(33624..34961)
                     /gene="fliI"
                     /locus_tag="ACHJ1G_000921"
     CDS             complement(33624..34961)
                     /gene="fliI"
                     /locus_tag="ACHJ1G_000921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4762819.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(34954..35664)
                     /gene="fliH"
                     /locus_tag="ACHJ1G_000922"
     CDS             complement(34954..35664)
                     /gene="fliH"
                     /locus_tag="ACHJ1G_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4762820.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(35667..36677)
                     /locus_tag="ACHJ1G_000923"
     CDS             complement(35667..36677)
                     /locus_tag="ACHJ1G_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4762821.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(36655..38301)
                     /gene="fliF"
                     /locus_tag="ACHJ1G_000924"
     CDS             complement(36655..38301)
                     /gene="fliF"
                     /locus_tag="ACHJ1G_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4762822.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLERELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(38306..38647)
                     /locus_tag="ACHJ1G_000925"
     CDS             complement(38306..38647)
                     /locus_tag="ACHJ1G_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4762823.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(38662..39648)
                     /locus_tag="ACHJ1G_000926"
     CDS             complement(38662..39648)
                     /locus_tag="ACHJ1G_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO4762824.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            40035..40886
                     /locus_tag="ACHJ1G_000927"
     CDS             40035..40886
                     /locus_tag="ACHJ1G_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO4762825.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            40879..41250
                     /locus_tag="ACHJ1G_000928"
     CDS             40879..41250
                     /locus_tag="ACHJ1G_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO4762826.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            41247..41999
                     /gene="fliP"
                     /locus_tag="ACHJ1G_000929"
     CDS             41247..41999
                     /gene="fliP"
                     /locus_tag="ACHJ1G_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4762827.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            42002..42274
                     /locus_tag="ACHJ1G_000930"
     CDS             42002..42274
                     /locus_tag="ACHJ1G_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO4762828.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            42276..43058
                     /gene="fliR"
                     /locus_tag="ACHJ1G_000931"
     CDS             42276..43058
                     /gene="fliR"
                     /locus_tag="ACHJ1G_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4762829.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLQALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            43048..44187
                     /gene="flhB"
                     /locus_tag="ACHJ1G_000932"
     CDS             43048..44187
                     /gene="flhB"
                     /locus_tag="ACHJ1G_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO4762830.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            44171..46264
                     /gene="flhA"
                     /locus_tag="ACHJ1G_000933"
     CDS             44171..46264
                     /gene="flhA"
                     /locus_tag="ACHJ1G_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4762831.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            46369..46647
                     /locus_tag="ACHJ1G_000934"
     CDS             46369..46647
                     /locus_tag="ACHJ1G_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4762832.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            46640..46996
                     /locus_tag="ACHJ1G_000935"
     CDS             46640..46996
                     /locus_tag="ACHJ1G_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4762833.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(47053..47826)
                     /gene="yafL"
                     /locus_tag="ACHJ1G_000936"
     CDS             complement(47053..47826)
                     /gene="yafL"
                     /locus_tag="ACHJ1G_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4762834.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            48012..48272
                     /gene="dinJ"
                     /locus_tag="ACHJ1G_000937"
     CDS             48012..48272
                     /gene="dinJ"
                     /locus_tag="ACHJ1G_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4762835.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            48260..48547
                     /gene="yafQ"
                     /locus_tag="ACHJ1G_000938"
     CDS             48260..48547
                     /gene="yafQ"
                     /locus_tag="ACHJ1G_000938"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO4762836.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            48703..49443
                     /gene="dpaA"
                     /locus_tag="ACHJ1G_000939"
     CDS             48703..49443
                     /gene="dpaA"
                     /locus_tag="ACHJ1G_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4762837.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(49414..50181)
                     /gene="yafJ"
                     /locus_tag="ACHJ1G_000940"
     CDS             complement(49414..50181)
                     /gene="yafJ"
                     /locus_tag="ACHJ1G_000940"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4762838.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(50387..50965)
                     /gene="lpcA"
                     /locus_tag="ACHJ1G_000941"
     CDS             complement(50387..50965)
                     /gene="lpcA"
                     /locus_tag="ACHJ1G_000941"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4762839.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            51205..53649
                     /gene="fadE"
                     /locus_tag="ACHJ1G_000942"
     CDS             51205..53649
                     /gene="fadE"
                     /locus_tag="ACHJ1G_000942"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4762840.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(53692..54138)
                     /gene="ivy"
                     /locus_tag="ACHJ1G_000943"
     CDS             complement(53692..54138)
                     /gene="ivy"
                     /locus_tag="ACHJ1G_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4762841.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            54319..55086
                     /gene="yafV"
                     /locus_tag="ACHJ1G_000944"
     CDS             54319..55086
                     /gene="yafV"
                     /locus_tag="ACHJ1G_000944"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4762842.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(55310..>56246)
                     /locus_tag="ACHJ1G_000945"
     CDS             complement(55310..>56246)
                     /locus_tag="ACHJ1G_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4762843.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT        12474 a        15294 c        14847 g        13631 t
ORIGIN      
        1 atctttaaca atctcaggtt aaaaactttc ctgttttcaa cggggctctc ccgctgaata
       61 ttcgcgcgtt aattaaaatc aggaatgaaa atgaaaaaga gcactctggc attagtggtg
      121 atgggcattg tggcatctgc atctgtacag gctgcagaaa tatataataa agacggtaat
      181 aaactggatg tctatggcaa agttaaagcc atgcattata tgagtgataa cgccagtaaa
      241 gatggcgacc agagttatat ccgttttggt tttaaaggtg aaacacaaat taacgatcaa
      301 ctgactggtt atggtcggtg ggaagcggag tttgccggaa ataaagcgga gagtgatacc
      361 gcacagcaaa aaacgcgtct cgcttttgcc gggttgaaat ataaagattt gggttctttc
      421 gattatggtc gtaacctggg cgcgttgtat gacgtggaag cctggaccga tatgttcccg
      481 gaatttggtg gcgattcctc ggcgcagacc gacaacttta tgaccaaacg cgccagcggt
      541 ctggcgacgt atcggaacac cgacttcttc ggcgttatcg atggcctgaa cttaaccctg
      601 caatatcaag ggaaaaacga aaaccgcgac gttaaaaagc aaaacggcga tggcttcggc
      661 acgtcattga catatgactt tggcggcagc gatttcgcca ttagtggggc ctataccaac
      721 tcagatcgca ccaacgagca gaacctgcaa agccgtggca caggcaagcg tgcagaagca
      781 tgggctacag gtctgaaata cgatgccaat aatttttatc tggcaacttt ttattctgaa
      841 acacgcaaaa tgacgccaat aactggcggc tttgccaata agacacagaa ctttgaagcg
      901 gtcgctcaat accagtttga ctttggtctg cgtccatcgc tgggttatgt cttatcgaaa
      961 gggaaagata ttgaaggtat cggtgatgaa gatctggtca attatatcga tgtcggggct
     1021 acatattatt tcaacaaaaa tatgtcagcg tttgttgatt ataaaatcaa ccaactggat
     1081 agcgataaca aattgaatat taataatgat gatattgtcg cggttggcat gacctatcag
     1141 ttttaatgat tattgccgga tgtgatgcat ccggcacatt tcactcacgc cgttaacttc
     1201 accggctcgt cacgaaaatc atccgccggt tccagcttca gattcagcgt cgtcagcagt
     1261 tcacgcagct tctcgtgaaa ctcacgcagg gtgtgctcca gtcgttcaac cacttcagtg
     1321 tcttttaccg gaacactctt ccagtcgcct gctttatcga acagaccaaa ctggtaactg
     1381 taggtaaaac gggattcctg cgcttcaagc tccatccacc agccccagaa ctcacgcact
     1441 tccggtgccg gtttcacgtt gacgcataca gccagacaat cgaaaaagaa tcgattatct
     1501 tcgcacttac cttcacgaat atacgggcct agtgcggtaa attttttgat caatctgctc
     1561 ttcgggtgtc cactcggtaa cgtcattgct atctcctgtt gtgatgcaac tgttttacca
     1621 aattggcaaa atttagcaaa tttttaacac aagcgttttt cgatccagcc ggtgatttcc
     1681 tgaagacctt tgtcaaaatt ccgatacacc gggttaaatg ggatctctaa taatttaccg
     1741 tcagcagatg atgaggtgat taagcgtgag tcctcttccg ggctgaacgg atcgttcttc
     1801 cagtagcctg ataacattgg cgttgggcag cgacgcccca gcaatccttg cacttttaat
     1861 gaatagcgat tcagctccac gcgtaacgcg tcatcggaag catcatgcat ccccaaacga
     1921 ctcgccagaa cgtcaagata catttccggc acctgttgct ggcacttaaa atcactcaac
     1981 agggtatgaa ctaccggacc aagacaggca accgctttca gacgcggcga ttcaagatat
     2041 gccagacgca cggcaacgtt agcgccgaaa cggaaaccaa aggccgcgac gcgagtgtga
     2101 tccacccacg gtacgttagg cagcgccttt aagacgtgct gatgcaacag gctggagtcc
     2161 tgggtgagct tccattttga agaaaagccc accgatggca tatcaatagt cagcatcgca
     2221 atgccgcgcg gcgcaaaata acgttcatac aggctgtaat agtccgtctg catcgcatcc
     2281 agaccaccac acattaatac tgtcgggaac gggccatcgc ctttcggcat atgcaaaaag
     2341 ccggtgatgg gcgcaccgcc gggtacggta aactccatct gccgcatcgt gcccggcaga
     2401 cgctgggcgg cctcttcata ggcgcggttt gataaagcct gcgcttgctc ggccaggtca
     2461 tctcctttca gatgaggata ggcggcaatg ttgtacaacg tagccgcatg cagccagtgt
     2521 cgaccactga gttgcggatc ttcttccgca caggcttttt gttgccacac catcgcctgg
     2581 gtggcccact cataaatcca gttgccacca cggtagccaa tcacagtatc gtataaatca
     2641 tcgtcggtac gttcagcatc gctcatcaca atacgtgcct ggacgtcgag gatttcacgt
     2701 gggtcaatac cgcgccagat ccacatcaga cggttaatca tgcgatacca gtgagggatg
     2761 gttttaccat caagagccga ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact
     2821 agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc
     2881 tgtgtcatgg atgcagcctc cagaatactt actggaaact attgtaacct gcctgaagtt
     2941 aaaaagaaca acgcccggca gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg
     3001 gacgaatacg acgcccatat cccacggctg ttcgatccag gtatcttgcg ggatatcaac
     3061 aacatagtca tcaaccagcg gacgaccagc cggttttgcg aagatggtga caaagtgcgc
     3121 ttttggatac atttcacgaa tcgcaacagc agtaccaccg gtatccacca ggtcatcaat
     3181 aacgatgaag ccttcgccat cgccttctgc gcgtttcagc actttaagct cgcgctggtt
     3241 gtcgtgatcg tagctggaaa tacaaacggt atcgacatga cgaataccca gttcacgcgc
     3301 cagtaacgca cccggtacca gaccgccacg gcttacggca ataatgcctt tccattgttc
     3361 agaaggcatc agtcggcttg cgagtttacg tgcatggatc tgcaacatgt cccaggtgac
     3421 gatgtatttt tcgctcatgt gaagtgtccc agcctgttta tctacggctt aaaaagtgtt
     3481 cgaggggaaa ataggttgcg cgagattata gagatctggc gcactaaaaa ccagtatttc
     3541 acatgagtcc gcgtcttttt acgcactgcc tctccctgac gcgggataaa gtggtattct
     3601 caaacatatc tcgcaagcct gtcttgtgtt gacaacattt tctgctaacc ctgtgacctg
     3661 caatactgtt ttgcgggtga tcgacaagga gacttaacgt gtctgaactg tctcaattat
     3721 ctccacagcc gctgtgggat atttttgcca aaatctgttc tattcctcac ccgtcctatc
     3781 atgaagagca actcgctgaa tacattgttg gttgggcaaa agagaaaggt ttccatgtcg
     3841 aacgcgatca ggtaggtaat atcctgattc gtaaacctgc taccgcaggt atggaaaatc
     3901 gtaaaccggt cgtcttacag gcccacctcg atatggtgcc gcagaaaaat aacgacaccg
     3961 tgcatgactt cacgaaagat cctatccagc cttatattga tggcgaatgg gttaaagcgc
     4021 gcggcaccac gctgggtgca gataacggca ttggtatggc gtctgcgctg gcggttctgg
     4081 ctgacgaaaa cgtggttcac ggcccgctgg aagtgctgct gaccatgacc gaagaagccg
     4141 gtatggacgg tgcgttcggc ttacagagca actggttgca ggctgatatt ctgattaaca
     4201 ccgactccga agaagaaggt gaaatctaca tgggttgtgc ggggggtatc gacttcacct
     4261 ccaacctgca tttagatcgt gaagcggttc cagctggttt tgaaaccttc aagttaacct
     4321 taaaaggtct gaaaggcggt cactccggcg gtgaaatcca cgttggcctg ggtaatgcca
     4381 acaaactact ggtgcgcttc ctggcgggtc atgcggaaga actggatctg cgccttatcg
     4441 atttcaacgg cggcacactg cgtaacgcca tcccgcgtga agcctttgcg accattgctg
     4501 tcgcagctga taaagtcgac gtcctgaaat ctctggtgaa tacctatcag gagatcctga
     4561 aaaacgagct ggcagagaaa gagaaaaatc tggccttgtt gctggactct gtagcgaacg
     4621 ataaagctgc cctgattgcg aaatctcgcg atacctttat tcgtctgctg aacgccaccc
     4681 cgaacggtgt gatccgcaat tccgacgtgg caaaaggtgt ggtcgaaacc tccctgaacg
     4741 tcggtgtggt gaccatgact gacaataacg tagaaattca ctgcctgatc cgttcactga
     4801 tcgacagcgg taaagactac gtggtgagca tgctggattc gctgggtaaa ctagctggcg
     4861 cgaaaaccga agcgaaaggc gcatatcctg gctggcagcc ggacgctaat tctccggtga
     4921 tgcatctggt acgtgaaacc tatcagcgtc tgttcaacaa aacaccgaac atccagatta
     4981 tccacgcggg cctggaatgt ggtctgttca aaaaaccgta tccggaaatg gacatggttt
     5041 ctatcgggcc aactatcacc ggtccacact ctccggatga gcaagttcac atcgaaagcg
     5101 taggtcatta ctggacgctg ctgactgaac tgctgaaaga aattccggcg aagtaattat
     5161 ttgatttgct gccggatggc gtttaatcgc cttccggcag tttcatcctt cattatcctt
     5221 cgataaaagc catccctgta aatgtccgtc tcgggttgcc acgttcaatc tggtgatgga
     5281 acattcgccg cagcgatttc agcaccgcgc tattttcctg ttgctgctgc tccagcttcc
     5341 aggcaatcag caatcgtgcc agccgcttgt tagcatgctg actacgctct gactgaacct
     5401 tcacgctaat accggatgcc aaatgcgtgg cgcgtaccgc cgagtcggtt ttattgacat
     5461 gttgaccgcc cggccccgac gaacgcaacg tctcataacg gattgcatcc gattgttcct
     5521 gctcatcagc ggtaaaacgc ccaatgccca gaaaccagtt tttgcgccca tgatgaggcc
     5581 gatacggact cggacaaatc cactgaatag tgccacacca gctttcgctt aatgcccatg
     5641 cgttatcgcc atccagagaa atcagcgccg aacgcagcgt gtcagagtag tggcccgttt
     5701 ctgtttccag caccgttacc gcgacgtctt gtcgggcagc ttctttaatt agcctgtcca
     5761 gcgccttttt cactgcgaga caacattctt ccggcccctg cgctgaggag agttgtagca
     5821 agatcatcct tttttccctc cactgttttt gagcgttagc accggacgca gccgcgcgac
     5881 aggaataatt aaaccggcct gcaccagaca ttgcaccacg ctttcagccg atttataggc
     5941 ttgtggcgct tcttcaaaga tgagttgttt atcgcgacaa attacccgac tgccaagttc
     6001 agtacgcgag agttgcgtcg ccgtgtattt cgctgccaga cgccctttac actcggtgcg
     6061 cccccattta cgccctgccc catgcgccag cgagtggagc gttttttcgt tcgcaacagg
     6121 ctgtacaatc caggagtaat caccacgtga accgggaata atcaccagac cgttgtcatc
     6181 cggcgtggca cctttacgat gcaaccagcc ctgttgatcg ccgatttgac acgcgctaac
     6241 aaagttatgc gccacatcca gaaccggact tcccgtggcc ttaacttgtt gcattatgcg
     6301 caaagcgatc atctggcgat taatacacgc aaacgccagc gcatcgtcat gttccgcaat
     6361 ataccgtagc gcgtcgtcac tgccttgagg caaaccatga tgcgagaacg aggcaatatg
     6421 ccgctgtaaa atagactgcc ccaccccccg cgagccgcta tgaaccaaca gttgcagatg
     6481 ttgtgcatcc agacctgcca gtgcaaacag ttcagcgttg atgatttgat caacttgttg
     6541 cagttcagcg aaatgattgc cgccaccgat agagccaagc gagctgcgcc acggatgctg
     6601 tgcaaatgct gacggcaggt tttcctccag ccagctttct tcagcaacgt catccagatc
     6661 tgataatcgc ttttcaaact tatcggcgtt gtatttgcga gcgagaatat ctgtttgcca
     6721 tagcgccata ccgcagccga tatcattgcc gaccagtgcc ggataaaaac gaccaacaga
     6781 gaagaacgct gcgccaatcg gatagccgcg tccgggatgt aaatctggca tccccactac
     6841 gcgctgcatg ttgggtaaat ttgccgtggt gtgtaattgt tggatcgcta aactctcgat
     6901 ccacaggtca tcagatgcga tggtaaatac cgcatcggat aagggacgaa tataattgcc
     6961 catataccat tccgtaaata gactcgtcat acttcaagtt gcatgtgttg cgacttgaat
     7021 tattgagagt aacaattaaa aaatcaggaa atggcagacg aatccagggc aagaaaaaaa
     7081 gaattaatcc tggaaaggtc tgcaaagagt cattgataaa ggatttgtca tgatttacct
     7141 ccttgcagtt tgtggaataa gaaaacgcgc acatactagg cttgcgttat tttcattgca
     7201 agctggattt aatgttgctg tttataacgc atataaaaat taataaacca ctctccccga
     7261 cattcaacgc gttgctgctt aactgtctga aaaccataac gttcaaaaaa gggttttgcg
     7321 gttatgcttg cgtccaccgt aagttcggat tcagacttaa tcaaaggttt taacaaagcg
     7381 ctggcaaccc cacggcgggt gtattcaggg tcaacaaata acatatcgat atagtgttca
     7441 acgcaggtaa taaagccgac cagttttgca ttaatgactg caacccgcac ttgtgatttc
     7501 gcgagtttct ccttccagcg agattcgtca atctgcgccc aggcggcaat ttgttgtggt
     7561 gaataatgct gactggcagt catcgtaacc gctctaatga aaatagcgca tagttgctga
     7621 aaatcgccag gctgatagtc tcttatttgt atgttattca taatcccagc accagttgtc
     7681 tttccatttg cgggtcaagc aacgtcacat gcagccccac cagtcgcaca ccgcgcccgc
     7741 cgcggcgttc atcccaggtt ttacgcgcgg tggcgattaa gtcagcttta ttcagccgcg
     7801 gccagacgtg ctcctgagtg gtttgctgaa aatcatcaaa ctttaatttc actccctgac
     7861 gggcgatcag taaatcaggt tttacctttg ccagacggcg ttcaagttcc ggatacagcc
     7921 gctcgataat cgcttcacat tcagaccagt gatgaatatc ttccgccatc gtgcgttcca
     7981 cgccgacgga ttttcgtaac cgttcgctgt taacgtcgcg ctcgtcaatc ccctgactac
     8041 gctcccacaa aatgcggcca aatttgccaa agcgtttaag cagcatcacc agatcacact
     8101 tttgtacatc accgcaggtc cgcagcccca tcgcttccag ttttgccgcc gagactttgc
     8161 cgacgccggg gatttttgcc agcggtaagg tttgtaaaaa tgccggaact tctgccggcg
     8221 taatcacaaa ctgaccgttg ggtttattca tgtcggaggc gattttggcg agaaacttta
     8281 ctggtgccac gcccgcagac gctgtcagtt gcagctcgct gaaaatcgtc tggcggattt
     8341 cctgggcgat gagggtcgca gaaccgtggc aatggacgct atcggtgacg tcgagataag
     8401 cctcatccag tgacaacggt tcaatacgcg aggtgtagcg cgagaagatt tcacggatat
     8461 gatttgaggc ttctttgtag gcgtcaaagc gccccggaag caaggtgaga tgcgggcata
     8521 atttgagcgc catccctgtc ggcatagcgc tacgtacgcc aaatttacgc gcgggataat
     8581 tggcggtgct gatcaccccc cgacgttcgc ggctgccgcc aatagcaata gggatatcgc
     8641 gcagggcggg attgtcgcgc atctccaccg ctgcgaaaaa gcagtccata tccacatgaa
     8701 tgattttacg cattactcac ctctcaacac tggtaaagta tacagtgatt tcagggtttg
     8761 agaaatgcgt aaagattcag catttaacgc gttcgctgcg aaagcacctg ctgcttatcc
     8821 agcttttgca ccagcggctg caccacttta tccccatgct gaccaaagta ttgatacagc
     8881 gtatcggacg cacttttggt cagcaccata atctcaatgc gccggttgcc cgcgctttgc
     8941 ggatttttgg aatccagcag catctggtcc gccattgcgc ttacctgcat cactttatct
     9001 tccggcattc cggcctcttc cagcacccga cgagccgaaa gcgcgcggtc acccgaaagg
     9061 ttccagttgt tgtagatatt gtttttgtaa gccatcgcat cggtatgccc ggtaataata
     9121 attttgttat cgagcgagtc gaaaactggc gcgagctcca ccagcagcgt tttaaagaac
     9181 ggcataatct gcgcgctgcc gcgttcgaac atattgcgat tctggtcgtc tttaatcagt
     9241 acgcgtaatc cctggggaac aatctccatc tccagattcg cttccatatg cgcatcacgg
     9301 gcaatggtgt tgatactggt tgccagctcg ccaagctcgg tggcggattt cttcttcagc
     9361 agcgccgttt tctcattcac atcacgcgct tttttctccg tctcttccgg caccgccacg
     9421 gtggcaggtt ttggcggatg agacgggctg attttattta acggcgacag cccgccaccg
     9481 ttaaaaatcg actgcccgtg cagcgcggcg ataatgcttt cacgctcgga tttactgacg
     9541 ctgttgacaa tccacagcgt cataaacagc gccatcatcg ccagggtaaa gtcggcgaac
     9601 gccaccttcc acgccccgcc atgatgcccg gcatgattct ttttgatctg ccgccggacg
     9661 atggtggttt ttgccccacg ctcgcggcgg ttgcccgtct ttctcatgct tattcttgct
     9721 ccagcatcgc attcacccac gcgtcgaggt tggcgaaggt cggtttggac gccagatgca
     9781 gcagcttacg cccggcatcc accgccagca gcgtcggttt gccgcctgcc tgcgccacca
     9841 gcacagtgcg cacgcattcc agcaaagaat gttctgcccg cgcctgttgt tccatggcgt
     9901 tggcgagcgg gtccatcagg cagtagcaga taaagacgcc taaaaacgtc cccaccagcg
     9961 ctgccgccac tttcaggccg atcagggcaa tagagccgtc aatcgactgc atggtgatga
    10021 tgatccccag cactgctgca caaataccaa atcccggcat cgcttcggcg gtacgttgca
    10081 gcgagcgcga cggcgtgagc agcgactctt ccgcagtgtc cagttcctga tcgagaatgc
    10141 cttccagttc atgggcgtcg attttaccca tcgccatcag gcgaaagtta tcggcaataa
    10201 aggtcaccag ccgcttctgg gttaacacca gcgggtattt ctggaaaatg gtgctctctt
    10261 ccggttgctc aatatgttga tccagcatgc gcagaccacc gttttgcacc atttcgagca
    10321 actcatacag gcacattaat agctgacgct gaaactccgg cccgagctgt tttttactga
    10381 tcacaccttt aatttgatgg gcaatctctt tcagcacatg tttcgggtta ccaataatca
    10441 tcgccccgaa accggcaccc aaaataatga tcatctccgc cggttgccag atagccacta
    10501 attgcccgcc cgcttcaaaa taaccgccaa atacgcagcc gagcaccacc agtaaaccta
    10561 atattttttg catgatttct tcactttaat tcggatagac aaaatcacga attttctgcg
    10621 cgatctgttt attcagttga caaattcggg cctcggttag ccctaatacc agcgcaatct
    10681 ctttgagatt catctcatgc tggtaataca tggagaggat cagttgctct ttttcgctta
    10741 attgtgaaag cgcctgttgc agcatttgct gagtgacaaa ctgatcttcc agctcccggc
    10801 caacaagcgg aatgcctggc ccttcgccgt tcagcagttc gtccaggctg gcgagcgttt
    10861 ccgccccttc cagttgctga tattcgagat aatcttcatg tgagatccct tccacggcca
    10921 gttctgacca ctccggttcg tgccccagtt ttttgcgcgt ttcgcgaatc agatctttca
    10981 tctgatgata tttctgtcgc agctggcgcg gtcgccagtc gagagcgcgc agctcatcca
    11041 ggatcgcgcc gcgaatacgg tgcacggcat agcccgcaaa accttcgtcc ggcagcccgt
    11101 agcggcggat ggcattaagc agccccatta acgccgtttg ttccatatcc tgacgatcaa
    11161 taatgcaatt acactgcggg gcaagttgcc gcaccacttt acgcaccaac ggtaaatagg
    11221 cctgcaagta gtggctctcc tgctgcgggg ttaataccgg cgcggcagca aattcttctg
    11281 actcgaatac atcctgtagc atgtttatat ccgcctggtg attactggaa gaccactttt
    11341 ttcaccagca aatccttata cggtgcgctc attttgcgac gctgaagatc tttctttaac
    11401 gcgtcagaaa tgagtttgcg gatggcagat attttcatgc cgcgcaaatc ttcaaatttc
    11461 atttccgtca ggcattcaac cgcaatactt tgatacagcg gctcctgatg ttttattttt
    11521 tccgcttctt tgtcactggc gaccaccatc actaattccg ccgacaaata atggtccgcg
    11581 ccgttgttgt catgcagcgt gacaatggtt tccggcaacg tcacaaacac gcttttgctt
    11641 tcatccaggg tttccaccgt ttccgtctgc gcggcggcag tcggttttcc ttttccggca
    11701 taatgatgcc agacacccca tccagctccg gcccccacca ccaacgccag aacggcgctg
    11761 accacgcccg ccaccacgat tttcttcata ttgcctttac actttgatca aaacggattc
    11821 gttctgttga aattgaggcg catcatcaag ctgtaaggcg gcactcacct cttcctgctg
    11881 atggcccgac tgttgttgtt gctgcgactg cccttcggag gaaacctgta cgttcacctc
    11941 cataaaattt tgcgccgtca gatgctggcg gagatcgtcg ctaaactgct gtaacgcgcg
    12001 gcaaacttca ctttggttcg cgccgatgtg caccatcagt ttgccgttgt cgagttgtac
    12061 ggcgatctcg agcttgccaa gcgacggtgg atcgagacgg atggtggaga tctgctgttg
    12121 tttgttgagc tgaaagtgga tctgatcttt caaaagcgtc gtcagttttt cgcccagttc
    12181 gtccatcgaa agcgtggctg gtttggcgac aatctcctga tgatcgctga gcagctccgg
    12241 cgtcttaacc tgcaacaccg cacgcgcaat aaccggttca ctgtccggca ctttttccgt
    12301 tttacgctcg acctgcgggc gcgcggtcac acgggttagt tcagatttcg ggcgagctgg
    12361 ctgtgttgag acgcgcggct gcgtcggcgt aatggcatgt aaatcttcac tagcgtaagt
    12421 cgccagtctg gcctgctgtt cgggcttcgc ctgcggcaac tgactgatca gttcctgcaa
    12481 ctgcggcggc aacggcgtga gttcctgctg ttgcgtctgg cgttgcggcg cgttttgcac
    12541 caccgctttc gtcaattgct ggataaccgg cgatgttgcg acgtttcgcg gctgcggcgt
    12601 gtcctgatgc ggcgcggcag gttgcggaag taacagcgcc attagcgcct gcatcgccac
    12661 cggatcgttc tcctgcgggc gcgtcgcctg ttgctgactg gtttttgcgc tatgaacgcg
    12721 ctccgccacc gccgcgaccg ccattttcgg cagggtaaac gcgggcgcgt tttcaccgct
    12781 caccggcggt aaaatatccg ccttcaacga agcggtttcc gcaagcgttc cgagcgtggc
    12841 aagcaatgtc ggattcatga cttctccgaa agtaaattaa caattgagta ggccagtacg
    12901 ccttcctgat gccgctgatg ctgttccata cgcgctttca acacttctgc gcgctgctcg
    12961 cgccgtttaa tcagggcgtt aaacgcgtca tacagtcgcg ctttcactgc atccaactgc
    13021 gccgcatcgg tttccgccgc cgcgtaatcg tgaaactggc gtatcaactg ctgatgcagt
    13081 gccggaaggc gtcgccagcg ttgcttgtcg agggcttcat tcatcgcctc gaccagtgcg
    13141 aaaagtttcc tgcgttgttt ttccatcgtc ttaacccagc ttgcccgaaa gcccttccca
    13201 gccttcgcgc aggttgctta agatcagcat cacttcatcg attttctctg ccgacagttc
    13261 gccgctggcc tcatacaatc ggtaaacgca gtggtcatag agtcgcgaaa ggttgaccac
    13321 cagctcgccg ccagtttcaa actccagcga actggtgagc gcattgagaa tgtcgatgca
    13381 tttattgatg ctctgggctt tcttctcgaa acgccgccct tcaatatggc ttttggcgcg
    13441 ttccagttcg tccatcaggc cagaaaacag caccagcacc agttccagag gcgatgccgc
    13501 cgcagtacgc gccgcgagat cgatctcttt atattgcgaa tagccgtcct gtacttggta
    13561 catctttagg gatattcctt ctgagttacg cgaatgccgc catgctggct ttcatggtgt
    13621 acacctcgac cagcgtgttg gtgtactcct cgagataacg gtcgtagttg gcgttatagg
    13681 tatcagtaag ctgatcgcct tcgtcctgaa ttttgctcat ctgatcgtca atgttctgct
    13741 ggcgcagggt gatgatgccg ttgctggagt ccgtgtaggt gttaataagg tcgtccatct
    13801 gcgccaccat gctgttgtcg ccaacgaaaa tagaggtcag accgtcagga tttttcgcca
    13861 tttcgtcgtt gaactggtcg gagtcaatct gtaagtggcc gtgagaatcg agggtgatgc
    13921 catagtcaac aatcgacacg ccgttgtagc tggcatgggc gatgtcatcg agctggttcg
    13981 ccagtgaact gagtccggcg tcgcctgcaa ataccccggc gctggtactg tcatcaccgt
    14041 gggtggttag cgaatcaacc gtatcaatca aggtgttata ggcatcgaca aaggtctgta
    14101 ctttctcctg gctggcgctg gaatcttcgc tgatgttaaa ggtagtgaga tcgctgtcag
    14161 aatcgctgac ttcggtgaaa gtcatggtga caccgggaat cacatcatca aacgtattgc
    14221 tgctgttggt gatctcaggc cccgtggcgc tgccaagatg aataatcgcg tcctgagcgg
    14281 aggaaacatc cgtggcaacg ggcgcgctgg tgctatcgtt gctggcatca tgccccgtta
    14341 cgctcaccga aaacgcgctc tgcgccccgg tgctatccga ggtgagcata atcgttgtgg
    14401 tgccgtcggt ttttaccagc gccgccgaca cgcccggatt atcatcggag tcgttaatcg
    14461 cattcaccag ttcactggcg tcgataaaac cgtcgccgtc accattctga tctgccgccg
    14521 acagatcgat atccatggtg ctgtcgccca tcgtcagttc aaaagtgccg gtggcggaaa
    14581 aggcgtcgtc gcccatactg aacgtggttt gctgcccctg tgccagttgc tcaacgaaaa
    14641 atgagtagct tcccgcctgc gcctgggaat tggcggaaac gctcgccgag tcattattgc
    14701 tggtggcggc aaacgtcacc gggccgtcgg tatcgctgtt cagcgcgtca acggcgctct
    14761 gaaaatcgct caacgctgaa ctgagcgaat ccaggccgct gctttcagca tccagctctg
    14821 tttgcttctc ctgcaagttt gccgcctgag tggcgacatc cgcatacgcg atttcttgcg
    14881 caatggttct tgggttgatc attgatgact ccggataacg atgtcaggtt attggcaaaa
    14941 ggcgtgccaa tattttttat gtgaataatc aatagattaa aaaacaggcg gaaatggcgt
    15001 ttccggtctg gcggaaaaaa gcctgccgca gaaagcacaa caccgcccgc aggcggtgtt
    15061 gttcgtagtc cgaataaggc gtttacgccg catccggcaa gtccgccatt actgcagcag
    15121 gctggaaacc atgctggaca tgctgttgga ctgtttcagc atggtgatgc cagtctgcat
    15181 cagcacctgc tgtttggtca tattggacgc ttccgtcgcg tagtcggtat ccataatgtt
    15241 accgatagcg acttcggtgt tgtcctgcat actggtcagg ttgttggcgg tgtcgttcag
    15301 acggttgata gacgcaccca gcttggactg gatctgcgaa acgtcatcca tcgcggtaga
    15361 gatgctggtg atcatctggt tagccgagcc agacgcggtc agctcagtac cgccagaaac
    15421 acctgcagta cccgtggtgt ctgcctgatc cgcgctaaag gagttgctga tagcagaaag
    15481 atcggtcacc agcgtgttca gttggctgga gatgttcacg gtcatggtgt cggaagattc
    15541 cgcgcccacc tggaaagtta ccgcgctctg gaacagaccg tcagtaccgg tgttgctggt
    15601 accggaaaca ccgaacaggt tggtgccgcc gtaagtggtg ttttgcagca tgtcagacat
    15661 ctgctgaccc agttcgtcgt attcgtcctg catcgcctgc agatcgtcgt cgctgtaggt
    15721 gccgttcgct gcctgagttg acaggtcttt catacgaccc agcacatcag acatttcatc
    15781 gaacatacta tcggcggttt gcagcatcgc ggttgcgtca ttgatgttgc tcagcgccac
    15841 gcccataccg ctggactgtg ctgtcagacg gttagcaatt tgtttgcctg cggcgtcatc
    15901 ggcagaagag ttaatccggt tacctgtcgc caggcgttcc atagatgtag acaaagaaga
    15961 gctgcttttg ctgattgcat taacggcggc cattgaggcg ttattggtgt taatagataa
    16021 catgatactg ctccttggga ttaatcgttt tcgtttcgat accctgtaaa aacgacacct
    16081 aaggagcaaa cttaaaaccg ccgttaaaaa aatttcactg cgatttatct gatgccactt
    16141 ttagctaaaa aaagcagagt ttaaattcag ataaaaagat tattagcaat atctgccaca
    16201 tggccaggta tcaaaatatg tctttatcaa acgcttgata tagttaagat taccatcaat
    16261 taacgcgttt tcttctcaat gtactgatgc caaaatttac attgcgttgc aaatagtaca
    16321 catttggcta ttgtaccagt cctgtcattt cgttacattt ccatgccata tagtgattaa
    16381 gaataatcct attaccataa aatattagtt atcacgcaga aaaatcatct tctcagggac
    16441 gtataagact gtgctttatg caaagcacta tcaggaaaca tttatccctt gctaaggtaa
    16501 actcaactat gagtattatt atcaacaact ggcggatgga cccgtcgctt aatgccttaa
    16561 tacattgcga aacgggtgaa acacgtcgtc tcggcgaata ccattttatt ttgctggaaa
    16621 aattagcaaa aaatgccgat gtggttttat cgcgatctta tttatgtgcc gaagtatgga
    16681 agaaccgcat tgtcggcgga aacagcctgc cgacggcgat ccatgcgttg cgcgttgcta
    16741 tcgatgatga tggcaaacaa cagaacatta ttaaaacaat tccgaagaaa ggttacttat
    16801 gcaataaaga gtatgtgtct ttgccggact catcgcctgc aaaaaaactg attattaccg
    16861 accagataca ggaaacagta ccagaagaga tatcttctac aacaccgcct gtgcctgtta
    16921 gaaaaaaaca taaagtaata atggggctag cgttagccgc ggctgttata tttattggct
    16981 cgaccgtggg gtattcacac ttgaaaagta cacctgatgc cccgcaactg gtaaaagagt
    17041 caattaacag tccaagaata aaaatatttc accttagttc tggaaaagaa aataattccg
    17101 taccgttact gtcacaaacc ctcgcaccag gaaaagacaa actcgataat ttattgtcag
    17161 cgcataatat gacaatgaca acctattata aatatgttcg taaccgactg gaaagtgata
    17221 ttgttctgcg caaccagtgc aatggtagct ggcaattaac ttttaatgtg gaaggctggc
    17281 agaacagcga tattaatagc gcgatgtatc agaatttaga gaagttgtta aatactttgc
    17341 agaaatgctg atgagtgcca atatatgccg gatgcgacgc taaacgcgtc ttatccggct
    17401 gataattacc ccaataaatt cctcaccgtc tggctggaaa ttcgcgcctg accattcacc
    17461 gcctgcaata aaacctgata gttatgattc gcgctatcca gcacgccgcc gaggttttcc
    17521 gacgcctgca ccgcgttttc cgtttgcggg aagcgcgaca tgccgtcgat caactgccgc
    17581 gccttttcct gctgtaccgc catctgcgca cgctgctgat taatgctttc taacgtctga
    17641 gaaattcccg caccgccctg ttgcaggctt tgagctaaac ggtctgcctc agacggctcg
    17701 gcaaaagttt tcagcggcat aaacgccgac ttatcgccat cggtgcgcac gcttaaggta
    17761 cgttcgatat ggggccactg tttttcggtg gtggagaaac tgatgccgtc cgcttgctga
    17821 tgtagctgca cgcccacgcg gcgcagggcg ttggtcagcc gcgtctgata ctgcgcggcg
    17881 ctgtcatcct cagacagcat caccgccgaa agctgagtct gccgcccgtc agagacgctg
    17941 aacatgcgcg ttcccggcgt gggattgtgt accagattcg ccagatccgg cgagtgaaag
    18001 gtcacccgcg cttcgccctg taacaccggc tgcaactggc ggtccaccgc accgccggaa
    18061 agcgcggtgc gtttgtccag catctgcatc agcgccgtat tctgcgcctg cccgccttta
    18121 cgctggctgt gacgatagtc gagcaactgc tgctccagtt gcccgagata atgatccgcc
    18181 tgctgcaacg tggtgagctg gtcgttaagc tgcacgctgt aacgctgcgg gcgagtggta
    18241 atcagcggcg aggccggata ttcactcgcc gtcgccggga gtttttgccg caccgcctga
    18301 gtgggcgcaa cggttgacga cgataccgca gcgctatgcg cgccgccggt ggcgagtgat
    18361 gaagatgtaa gtccgacctg catactgttc ctgaaagagt tagagaacgc tgaacaaatt
    18421 caactcactc actttcgagt aagtcttgag ggtaacttcc attgccactt ccagtccggt
    18481 aaaggtgatc gacgccgggc cgtaatcgag atcctgtaat gaaccaatca gctcatcatt
    18541 agaggtggag atatcggtct gcgcatcgct cagcatcgac atggtatttt gcgtctcgcc
    18601 gagtgacgca atcgaggcgt tgaggtcgtc cgaggcggtg tccaccacat caacggcatt
    18661 ctgaatatcg ctctgcacct gcggatctgc cgggtcgacg tccgggtttt gcaactcctg
    18721 ggatagggaa ttcagggtgt tgagcacgtc taaattgctg ccaaagaaat cactcgccgc
    18781 cacgttggtg tccacttcca cgccgttcga caccgtggtc tggcggtggt cgctgttacc
    18841 ctgatagctg tagctgtcgg tgacgccatc gccgtcatcg tccactgcca caatcggcgg
    18901 ctgatcgttg atggtgccgc caaacacgta gtgcccgtct tcgtcctgat aattgagcgc
    18961 cgccaccatt gattcggtga gcgactcaat atcctgcccg aggcttgcga gtgaatcggc
    19021 ggtattggtg ccattcgccg cttccagcag gtcgtcgcgc accgccagca ggctgttgtt
    19081 gacgccatca aggatcgact cctgctggct taagcccgcc gacgcggagt cgatattgct
    19141 ctgatactgc tcaatcgccg actgctggcg gtttaactgg gtgattcgcg tggcggcgat
    19201 cgggtcatcc gacggctgca aaatctgctt gccggttgcc atctgctcga caacatgcgc
    19261 cagatcgcca gagagattat taaaactttg cgtcatcgag acgtaggttt gttgggtagt
    19321 tactcgcatt ttcgcgctcc cgcttagctg cacatttcca gcaccgaatc gaaaatctcg
    19381 gccccggcgg aaatgacttt cagattggct tcataaattt gttgataggt gatcaggttc
    19441 accgcctctt cctccatgct gatgccgctg acgctgctct gctggttttg cgctgcggaa
    19501 tagacattgg acgcggcatc gacttccgtc tggttttgct gactgtaaat gccgatattg
    19561 ctgatgattg acgagcacgc ctgcccgacc gtcacgctgc cgaggttggc tatttccagc
    19621 ggttcggtgg agatattgat cagcgcctgc aggttgtcgc tgttgccgct ttcgtccggt
    19681 gaactggaaa acgccagctc atcggcggtg atattcgggt taacggtcag cgggccatcg
    19741 gcattgctgg cgtcataaat aaacagcggc tcgccggggt taccgttgag atcgtagccc
    19801 tgcgccagct ggttgttgac cgcatcggcg aactgcgacg ccatgctgtt gatggtgtcg
    19861 gtcagcggcg tcagcacatc gttttgataa tcaaacagtg cgcctaatga accgccggtg
    19921 tcggtggtca tcgtcgaggt ggtgccagcg aaggtcagcg acatggtcga cgtgccatcg
    19981 gcgttggttt ccagcgcgat ggtcgaactt tgctgcccac tcaccagcgg ctgaccgttt
    20041 ttcagggtaa cgttgtagtt gccctggtcg tcgatattga cctgcacatc catcatcccg
    20101 ctcagttctt ccaccatctg gtcgcgggcg tcgtacagcg cggaggcgtt atcgccattg
    20161 gcttcagcct gagcgatttg ctggttatag ctggcgatgc cgctggtgag cgtattgatt
    20221 tgcgacacca tcgcctgctg ctggctgatg atttccgtgc tttgcgagtc gatgtaatcc
    20281 agcgtgttat caatacgcaa cgacagcgcc ccggcttcac tgatcacctg ctcgcgcagg
    20341 gcggaatcat cggggctggt ggttgcttcg ttaagggcgg cgaagaagtt atcgaagccg
    20401 ccgctcaggc tgctgttatc gtcgctcagt accgcttcca gttggctgag atccccctgc
    20461 tgggtgctgt aatagccgta atcgctggcg gcataccaca cctgattcac ctgatactgg
    20521 ttggagacgc ggcgaatgct gtcgacctgt acgccgttac cggcgctgtt cggcgaaccg
    20581 ccgctggcac cgatggtgct gatctccgcc acctgacggg tgtagcccgt ggtcatggcg
    20641 ttggcggtgt tttgcgccgt gacgttaagc tccacctgcg cggttgatgc accgctgtag
    20701 ccgatgttaa tcatgtccat gttggatcct taccggtaaa tcgtgccggt taaaagcggc
    20761 gtaaagttgg ggattcttaa gttccgtaaa ccgggtgccg aacgccggat gcggcgtgga
    20821 cgccttatcc ggcctacata cgggcgactc gtaggcctga taagacgcga cagcgtcgca
    20881 tcgtgcaccg aacgccgaag ctcatttatg cttcggcgac aactgcttca caatcatcgc
    20941 cgcaatgcca atcccgtgct gctgcgccag ggtgttacac aactcatcgt cataaaaccc
    21001 ctgcatcatg cgcaccgaat cgctgttaaa cgggttatct tttgaatcaa acaactcgtt
    21061 ggttttccgc atctctttca gcatgttgcg taaaaaaatc gcctcaaact gttcggcggc
    21121 ctgcttgata tcattggcct gaaccttcgg ccccattaac gcgtcgctgc cgtcaatccc
    21181 gccgctggcg ttcactttca tcagatcacc tccaggtcgg catccagcgc gcccgcctca
    21241 tgcagcgcct gcaaaatcga cataatgtcg tccggcgtcg cgcctaaact gttcagcgca
    21301 ttgacgagcg ttttcaggct gctcgactcc ggcaaactca ccacgcccgg acgggcatga
    21361 ttcaccgcga tattgctctg cggcgtaacc gccgtgcgcc cacccgcaaa ggcattcggc
    21421 tggctgacgt tgctggtttc gttaatcgaa acggtcaggc tgccgtgcga caccgccgcc
    21481 gcgtgtagcg caacgccatc gcccattacc accgtgccgg tacgcgagtt aaacaccacg
    21541 cgagcgcgga ttttctccgc ctgcacctgc acatcgtcca gttgcgacat aaatgccacc
    21601 cgcgcgccgg ggctggtggg cgcgcgcacg gtaacgttgg tggaactctg cgcggtggca
    21661 ataccgccaa acgaggcgtt gatcgccgcc gcaatgttgt tggcgtcttt aaacgacggg
    21721 cgtttcaggt tgagggtgat ggtatcgccc atctgaaaat cgctggggat ctcgcgctcc
    21781 accgttgcgc cgttaggaat taacccggcg gttggcgtat taaccgttac gctggaaccg
    21841 ctggccccgg tggcgttcat cccgcccacc accacgctgc cctgtgccag ggcgtaaact
    21901 tccccatcag cgccgtgcag ttgggtcagc agcaaagtgc cgccgcgaat acttttggca
    21961 tcgccaatcg acgaaacggt gacatcaatg gtctgcccgc gcgagtacat cggcggcagc
    22021 gtggcgctca ccgccaccgc ggcgacgttt ttcaccttcg ggtcgatttt attcggcagt
    22081 tgcacgccaa actggcgcag catattggtg atggtctggt tggtgaattt gacctggttt
    22141 ttatcaccgg tgccgtcgag gccgaccacc aggctatagc ccaccagttg gttttcgcgc
    22201 acgccctgta cgttgaccag cgactccagc gattgcgcca gcgccacacc aggcagcgcc
    22261 aggctcacgg cgataactgc ggcctttatc cagttttgca ttgcactctc cggcattaaa
    22321 tcgggaacag aggatggtta aacagccgcg tcagccagcc tgcggcgttg gcgtcgctta
    22381 acgcgccgcg tccggcatag gagatgcagg catcggcgat ccgctgggag gaaaccgagt
    22441 tgtcgttctg gatgtcgtcg gcgcgcacca ggccgcttaa gcggatgtac tcatcgccct
    22501 ggttgagggt gagccatttc tcgccgcgaa tttccaggat gccgttgggc tgcaccgcat
    22561 ggacggaaac ggtgatctcc ccgcgcaggc tgttctgctg ctgggaagtg gcgctgccgt
    22621 caaagtcgcg gttggcgtcg atagagccgg aaagcttatc tttagtgtga ctaaaaatgg
    22681 tcggtgcgcc gatatcgacg gtgttgcttt tgccgaagtt ggttttcgcc tgtttgctcg
    22741 actgggtgga ttcttcgagg atcaccgtca ggatatcgcc cacgcgataa gcccgccgat
    22801 cggcggtcag cgaccagttg tagccagttt caaatacacc gccagcacgc ccgtctgccg
    22861 gaggcggtgc ttcggttttc ggtggcgcaa agtatgcgtc gtcttttttg accagaatgg
    22921 cctgcgattc acacccgcta agcaggggaa gcagcgccac cagccagaga taatttttca
    22981 cgttctgcct gtttaaatac agtgagttat gcgcttttgt aggtcggata agacgcgtca
    23041 gcgtcgcatc cgacatttcg gccttacagc gtctgactga tgtactgcaa catatcgtcc
    23101 gcagccgaca ccattttggc gttcatctcg taagcccgtt gcactgtaat catcgccacc
    23161 atttcgttga cgatatctac gttcgagcct tcgagggcat tgtcctgcaa agtgccaagc
    23221 ccatcttcgc ccggcacacc ttcggtcggc tgaccgctgg cggcggtttc cagatacagg
    23281 ttgtcaccct cagcggaaag cccagccggg ttagcgaagt taaccagcgt cagttgccct
    23341 aactcggtca cgtcgctgtc gccaggaaga atcgccgtca ccgtgccgtc ctcaccaaac
    23401 gcaacattcg tcgcccccgc cggaacgtcg atttccggct gcaatggcaa gccctgtgag
    23461 ttggtcagta cgccatcggc gttcacctgc aaattgccgt cgcgggtata ggcgatatcg
    23521 ccgttggcct tttgcacctg caaaaatccc tgccccataa tggcgacatt catcgcgttg
    23581 tcggtggttt cgacgttgcc ttcggtgaag gttttctggg tgccgacaat gcgcacgccg
    23641 ctaccgaatt gcagcccggt cggcatgatg ttgttctggt cgagcatcgc gcccggcgct
    23701 tcctgggtct ggtagaacag atcctgaaac atcacccggt cacgcttgaa gccggtggtg
    23761 ttaacgttgg cgatgttgtt ggcaatcgcg ctcatttcag catcctgcgc cgagaggccg
    23821 gttttgctga tccataatgc tgcgttcatg gtggtaaatt ccgtgttatt tttgggccgc
    23881 ggttgccgga tgcggcgtga acgccttatc cggcctacga tgtgcggtta cgagccgcgc
    23941 aataagcggt tgcccgcgtc gctgatatct tcggcggttt tcatcatctt gatctgtgct
    24001 tcgaactggc ggttcatggc gatggacgac atcatttcgc ttaccgccga gacgttgctg
    24061 ccctcgagaa aaccgccgct gactttgatg ttttcatcgc gctgggccgg aacgccgtcg
    24121 gcagtcacca gcatcccttc agcatttttc tccaggttag ccaccgggat atccaccagc
    24181 ttcaggcgat cgatatccat cgtcgcggtc acatcgccgt catcgggcgt caccgacagc
    24241 gtgccgtcgc tgccaaacga agcaatggcg ttcggcggca gaatgatcgg cccgttatcc
    24301 cccagcacca gattgccgtc gatggtcagt tgcccctggt cgtcctgctg aatgttgccg
    24361 ttgcgggtat agacttcgtt accgttttta tcctgcaccg caatgtaacc cgcgccctga
    24421 atcgccacgt ccagcgggcg ttcggttttc tccgcaacac ccgtgctgtc gttcacgccg
    24481 ctggcccctt cctgcgccat atagcgcgtg tcgaagccgc cgcctttcac cttgtcgttg
    24541 gttgccattg ccatatcggc gcgaaaacca ttggtgttga cgttcgccag gttgttggcg
    24601 ctaatttgct gctcatacag ggtctgagaa gcaccgctca gggcggtata aatcaggcgg
    24661 tccattacat tgcctggaac agcgcgtcat cgagctgcgt gctggtggcg ataactttgg
    24721 tgttggcctg atagttacgc tgggcggtca tcaggttgac cagttcgctg gtgatatcga
    24781 cgttagagct ttcgagcacg cccgacgaca gcgtaccgca ggtgccggag ccgggaacgc
    24841 caatcagcgg ggtgccggat tcgcccgttt gtacccatgc ggtgccgctc actgcctcca
    24901 ggccgttttc attcgggaaa gtcgccagca ccacctgccc ctgcaacatg cgctcgccgt
    24961 tgctgtaggt ggcgtaaact ttgccatcgt catcaacctg aacaccgttt tgcgtggcgg
    25021 aggcgtagcc gttggcggcg ttggtggtga ccgaaaattc agagccgtat tgcgtacagg
    25081 tggagtaatc gacggttaag tcgacgggcg cggcggcgtc ggtttgaaat tcaatggtct
    25141 gcggcgtggt tggagaggtc agcttcccgg tgttcggatc gaaggttaac gtcgtcgccg
    25201 gaacgccggt ctgctgctga ccgtcgaagg tgtactgcac ttcccaggtg ttgtcgctgg
    25261 ttttggtgaa gtactggcat accgaatgtt cgttgcctaa tgagtcatac accgtggtgg
    25321 tgtagctgtc ggtgtacgaa ctgctgttag tcgcgtcgaa cggaacggtg gtgcgatcga
    25381 tagcttcatc gctggcgttg aagttagcgg taaaggtcag gctgctgctg gcctgcgccg
    25441 ggatattgcc ggttttgatc tggatatcgg tgaccgtacc ggtttgcaga gtgccagtgt
    25501 catcgaccgg atagccctgc aaataagcgc ccgaggcgtt gacgatatag ccgtttttgt
    25561 cggtttcaaa cgatccggcg cgggtatagg agatattgcc tgcgctgtca cacatgacaa
    25621 aaaatccgtc gtcgttaatc gctaaatcga gcgcattgcc ggtggagacc agcgaaccgc
    25681 cacgcgaaat actttgcgcg gtgcccgcca cgctgacgcc catcgcctgg cttccggcat
    25741 acatggcgga aaactgggtg gtcatcgact tatagcccac cgttccggcg ttagcgatgt
    25801 tgttactgat cccgtccagc tgttcgttaa cggcattcag ccccgttgcg gcaatttcat
    25861 aactcattgt gtcttcctgt taattgattc ttacgccaca tcactgtcgg catcgggtac
    25921 gccaaactgg ctaatcatgg tgaacggcac ttcgcccacg cctgcaacgt taagcaccgg
    25981 ggaactgccg tcgagcggga tgcgcacatc ttccacttcg ccggagactt caatcggcac
    26041 ctcttcttcc ccggtattgg tcaccaccga gacgtcgtaa tcgcccggcg ggatgccgta
    26101 atccgcggga ttcaccgaga aatcaacctg gcctggtccg acggacgaac tgccttctgg
    26161 gatcagcgag acggtgtagt catcgcctgc ggcatcggta aagtggagat cggcggtggt
    26221 acaggcgtcg tccagcgtag cgcgaccgtt aatggtctgg tcgctcacct gcaaggcggt
    26281 ggtctgcacc ataatgtcgt cgcccaccag gttaccgagc gccatcacct gaatgttgct
    26341 catcagcacc gcgttggtgt tggcctgcac gctcatctct tccatcatcg ccacctgcgc
    26401 catcgacgac agctggtcga tgtattcggt agcgtcggtc gggtcggtcg ggtcctggtt
    26461 ttggatctcg gcgacaaaca gcgtcaggaa ggtatcgaca ggcgagctat cgctgctgct
    26521 ttgcgtgtcg ctggccgcca cattattgct ttccgccgtg gcggtcggct gcggcgattg
    26581 cgcattcagc gccagggtat tcatcaggct tctcccagtt tcagcaaact ttgctgcata
    26641 cttttcacgt tgttgagcac atcgacgttg gtttcgaaat cgcgcgaggc cgacatcatg
    26701 tccgccatct gctccaccac gttgacgtcg ggataccaga cgtcaccgtt ggcgtcggcg
    26761 agcggagaat gcggttcata gcgtttcacc gccccgccgg tttgcagcac gtcctgtacc
    26821 tgcacgctgg caccgtcaat ggcatgacga tgtgtacccg ccagcaggct gtggtgatac
    26881 accgcggcaa acaccgggct gcgcgcttta tacgcctgcg cctcgctgct ggcgggcgca
    26941 ttggcattcg ccaggttact ggcaacggta ttcagacgta gcgtttgcgc cgtcatcgcc
    27001 gagccggaaa tctgatagat atcagtaaat gacatgctta tttcccttca atggcctctc
    27061 tgatgccgct gatttgcaga ttcagaaagg tcaaactgct ttgataatcc atactatttt
    27121 gtgaaaaccg cgcctgctcg ctgttcagtt ccacggtgtt gccatcttcc gacggctgca
    27181 tcggcacgcg gtactggaca tccaccgccg cgttatttgc ccgttgcatc tccctggcaa
    27241 aatcaatatc tttagccttg aaatttggcg tatcgacgtt cgccagattc gcagtcagta
    27301 gctcggtcct ctcaagacgt aacttcactg cctgcggatg tactcccaat gcctgctgaa
    27361 aactgatccc catttcctgc atcactcctg cttcaatccg catacgctaa gcaatcgcca
    27421 tgccattttt taagtcactg atgagaaagg gtttatgagt tttcaatttt gcaggcggaa
    27481 gttttacttc ctcctggcgc tggccttacc gggctacgcc gccgtccatc ccgtgcaaca
    27541 cagcgcgcgg gaacaagtca acgcccaggt gctgaatgcc gcgagccaaa aaattgaatc
    27601 gctggcgcag cagcggcagt ggcatgacta tcgctacacc tttaagatct acattccctc
    27661 gcagattgcg accgccgcgc cctgtactaa aacacccggc gtcacgctga cttcgcctgc
    27721 ggaaatcgcc ctcaaccgga tgaacttcac cgttagttgc ccgcaaagct ggcagatgaa
    27781 cgtggcggtg cgcccggatg tactggtgcc ggtggtaatg acgaagtcac tggttgcccg
    27841 cgatatgccg ctcaccgcga atgatgtgga gcttaagccc tacaacgtca gcgcccagcg
    27901 ccgcgatgtg ttgatggagc ttgatgacgc gattggttta agcagtaagc acgcattgca
    27961 accaggcaga cccatcacca aagaagaact ggtgtctccg gtgctggtcg ggcgcgatca
    28021 gccggtgatg attgtttacc agagcgcggg gatcaccgcc tcgatgcccg gcgtggcgct
    28081 gaaaaatggt cgtaagggcg agatggtgaa agtgcgcaat gccagcagcc agcgggtcat
    28141 cagcgcgatg gtggcggaaa gcggcgtggt gacaacggta agcgcggagt aatttgcgca
    28201 ggcgattaaa gttttgccgc ctgttaaccg ctttaccgca gtaacccacc caaataccga
    28261 gacgaggttg cgatgaaaat caccccgact atgcctggca atcgcccgac agccacaacg
    28321 gcaggctcca gccagccgcg caaaaccacg gtgaccacca ccacgacgct gagcgccgac
    28381 gacattactc aggcgggatt gcagtctgcc cagcaaacgc tgaacgacca gcaagagagc
    28441 gatatcgaca gcaacaaagt ggcgcaagtg caggcgatgc tggcgtccgg cagcccgcaa
    28501 gtggacactg aacagttagc cagcgacatg ctgagttttt tccagaacta agaggccgat
    28561 gaagtgagta tccggttaca acaggtgaag gcgttgttgc agggcattcg tgacgatgac
    28621 gcgctgtatg attccctgcg cgaacttctc cagcgtcagc ggatctgcat gattcgccgc
    28681 gccagcgaag agttgctggc ggtaaacgat gagataaccc atcactatga gcagttgcat
    28741 ggacatagcc atcagcggca tagcctgctg aaaatgctcg gtgttagcgt aaatcgtgac
    28801 ggtctggcgc aggttttcac ctggcttccg gctgttcaaa aagcggctgc acaacagttg
    28861 tggcaacgcc ttgagcaaaa agcggaacgc tgcaaagcct acaacgacaa aaacggcgag
    28921 ctgttgattc gccagtatga atttattcag tcgtttttag gcagcgaagc agattttctc
    28981 tatcaggaat aacgcgaata ccttccccat agcggaaatg gattttccgc ctgatttcca
    29041 atcccttgtt tttaattaca aaaatccccg gcacgcttat tgcatatcct gcacagcatt
    29101 aaagactgga agccacctat gagttttatt gaatgttacg aaccggaata cgtgcgcaaa
    29161 tttttagcgc gtccccccgg ttcgccttta tacgtccgca aggtgtggaa aaacgagatt
    29221 cgcctgagcc tgtcattgac cgatattgcc agctgcgaag cggtgctcaa cgatgtcagg
    29281 gcctttgatt tacagctcac ctaccgccct gtggaagata attccgcaga gctttccgcg
    29341 cgcgatgcga tagtaaacca ggtgattttg agtaccctca cgctccgtga tttaaccccg
    29401 gagctttccc tgtatgctgt cgggattttg ctctcctgcg ccagcaaaat gccgggcaga
    29461 gacggagaca tcctggcccg tttgacgacg ctgccacagg cgctggccga tcatgcgaaa
    29521 aaaggtacgc tacaggcaca gtacgcccag ctcccgcccg tgccacagct ggcgcgccag
    29581 ttgatgacgc tgctcggcgg ttgtgcgttt gactggtcga ttttgccgga aagcccgcgc
    29641 aaagcctcgc tgccgttgca ggttacttta ctgacgctgc atgacgccaa cagcgaagcc
    29701 ctgttacaac agcagcttca gacgcagtgg caaaccacct ggcaacagca ttttgccacc
    29761 gcgccctgga tgatgcgtaa ctggctgatt taccgcgttt accacgatat gattgggcag
    29821 actgacgggg cggattattt cccgctggtg tgcgattttt atctcctccg cacgttaatc
    29881 agtttatgga cgctggatgg ttctccgctg cggcaggagg atattttcgc cctctttgcc
    29941 atgtttgaac gctggcgagc cagtgaaaat gcgctgttgg ttcgccagca aattcagtcg
    30001 ttatgtgccg ccgacccgct gctttcagcg ttttcgctgc taacgtgagt cgctctgcgc
    30061 gcgctttgcc cctcacccta accctctccc caaaggggcg aggggaccga tcgagcacgg
    30121 tgttaaaatc ggcgctgatt ttctccctct ccctgtggga gagggtcggg gtgagggcat
    30181 cagcgcgcac gttcatcaca ttcacacatt aactcaccca aacaccatgc caacaccaac
    30241 catcatcaaa acactcgcca cccaccgcga aaaaccgttt gtcagcgtcg tcacgccaac
    30301 ctggaaccgc ggcgcattcc tgccgtacct gctctatatg taccgctatc aggattaccc
    30361 ggcagaccgc cgcgaactca tcatcctcga cgattcccca caaagccatc aacacatcat
    30421 cgaccgcctg accaatggca cgccggaagc ctttaacatc cgctacatcc atcacccgga
    30481 aaaactgccg ctgggcaaaa aacgcaacat gctcaacgaa ctggcgcgcg gcgaatacat
    30541 cctctgcatg gatgatgacg actactatcc ggcagataaa atctcttaca ccatcgctat
    30601 gatgcaaaaa caccgggcgc tcatctccgg ttccgaccag atccccatct ggtacagcca
    30661 tatcaaccgt atctttcaaa gccgcagctt tggcccacat aatatcctca acggcacctt
    30721 ttgctatcac cgtaactacc tgaaaaagca ccgttacgac gatgattgca atttaggcga
    30781 agagaaaagt ttcaccgaca atttcagcgt taacccgctg caactgcccg gcgagcgcac
    30841 gattttgtgt atctcgcata gccacaacac ctttgataaa gactttatta tcggggccag
    30901 cacgccggta aacacggcgc ttacggatat cgtccgcgat ccgctgctgc gcaatgctta
    30961 tctgagtctg cataacgcca ctcatcacca ggcgatcaac catcaggcga tcgatcaggt
    31021 ggtcgtcgtg aatctggata aacgcccgga tcgcttacaa cagatccgtg aagaactggc
    31081 attgctgcat attccacccg agaagatcac ccgccttgcc gccagtgaag atgaaaacgg
    31141 tcaacggggc cgccgtcagt cgcacttgca ggcgctgcgt ctggcgcaac agcacggctg
    31201 gcaaaactat ctgttactgg aagatgacgc ggttattctc aagcaggaga aacatattca
    31261 ggtactgaac acgctgctgg cgagcctggc gaaaattccc tggcaggtga tgatcctcgg
    31321 cggcgaaatt tcgcaaggaa cgatgctaaa aagtttgcct gggctggtgc acgcgcggga
    31381 ctgccgcaaa gtgtgcgctt atctggtgaa cagccgctac tacccgcaac tggcgcagca
    31441 gatgagcaac gatgagcact cgctggaaga ctgctggcaa ccgctgctac gcgccgacaa
    31501 atggctggcc tgttacccga gcctttgtta tcagcgaccg ggctttagcg atatccagaa
    31561 aaaaacgact gataatattg ctcgctattt caataagtta cccgtaacca ccaagccatc
    31621 cgcgttaccc atcgccgaca ccatcggctt ttttatggaa acctcattcc actacgcgct
    31681 gtaccgccca atcatcaccg ccctacaggc gcaagggctg agctgcacgc tggtgatcaa
    31741 cgaccgcgtg tttaaaccgt ttctggatga gatgctggaa acactgaaaa acatcgacga
    31801 tccgcagctt aaaggtatgc gtctgtcaga aatgcagaca catggtcagc gggtgaaatg
    31861 cctggtcagc ccctaccaca cgcctgcgct caatggcctg gcggcggtga acatccgcgc
    31921 tatgtacggc ttagccaaag agacgtggaa ccacgccgac tggaatcgtt tttaccaaag
    31981 cattttgtgt tatagccact acagtcagca ggcgcttgcg catttcggca gcgcaaaagt
    32041 ggtcggcaac ccgcgcttcg acgcctggca taacggcacg ttcgatcgcg cgttgccgga
    32101 aaacatccaa tcagattacc gtaaacctac ggtgctctat gccccaacgt ttggcgcatt
    32161 aagctccctg ccccactggg ctgaaaaatt gggccgcttg agcggtgatg tgaacctgat
    32221 ttgcaaactg caccatggca cctgctcacg cccggaagaa gcagcatcgc tggccctggt
    32281 gcgcaggcat ttaaaacagc gcaccgactc cgcccgccac acgctggcgc tgctggcaaa
    32341 agccgattac gtgctgaccg ataacagcgg gtttattttc gatgctatcc acgttgataa
    32401 gcgggtgatc ctgctcgact ttccgggcat gaatgatttg ctcgacggtg agaaaagtta
    32461 ttccacggca gagagcgccg accagcgaat ccgcgaaata ttgccggttg ctcatgatgt
    32521 tgcagaactg cgttatttgt tgtcagaggc gtttgactgg gggtcagtgc aggcgcggct
    32581 tacggagatt cgccatcact attgcgatgc gtttatggat ggcaaggcgg gagaacgggc
    32641 ggcgattgtg attgtggagg cgctggggta aaccgcgcaa ctgcctgatg cgcttctctt
    32701 atcaggccta cggatcgatc acgtcctgta ggccggataa ggcgtttacg ccgcatccgg
    32761 caagaaaata catctctgtt ttagtacttt ataaaagccc tctaatcacc tcaataatcc
    32821 ccgtcgtcga caccgacggc gtgcggggga aatagaccac ctcacaaata tacgcaaagc
    32881 tgtcaaactt ccctgcccag tcatccccca tcactaaaat atccgcactg tacccgcgca
    32941 aatactccgc tttctgctct agcgattctt ccagaaatac gccatccacg cacgccagcc
    33001 cggcgatgat cgccatgcgg tcatcctgat ggtaaaccgg tgccctgccc tttttggcga
    33061 tgttcagggc gtcggacgat acgccgacca gcagccgctc gcccaatgcg cgggcgcgtt
    33121 gtaaaaggcg cagatgaccg acatgaaaca catcgaaggt gccgaaggtg atgaccgttt
    33181 tcatggctac tccgctaatt tgtgccgcag ccagcactgc acggcgatgt cgtccaccgc
    33241 tttttgcgct ttcgccgtgg cgctttcttg ttgctcgcgg cgtttgtcgt ccagcgtcag
    33301 ggcgactact ttctcttcgc acgccgccgc cgtgaggttt ttctgcattc gcgtggcttt
    33361 gatattggcc agcgtttttt cctgctcctg ccaggcgatt accttgcgca gggtgccttt
    33421 gtagccgctg acgtttttca gcgattccac cgacaccgcc gcacccgcag atgttttctc
    33481 gacaaagtag cctaacgcct tgatattgtt gtcataaccc gcgcaaatct gtttttgctt
    33541 tgccagctcg acggttttgt ctttgaccga tttatcccgc aggcgctgca actgcgtcag
    33601 ggtgtcgatg atttgtcgca tagttactgt tctgcctttt tgacaccagg aaagagaatt
    33661 tgcaggcggc tttgcaccag ttcgagggag gcgggttcgc gcatctcctg gcgcaaaaat
    33721 cgctcgatgg ccgggaacat ttttaccgct ttgtcgacgc tggcgtcggc acctgccaca
    33781 taaccgccga gcgggatcag aggtttgatc tccatatagg cggcatagtt ttgctttaac
    33841 gcgcgggcgg attgctgatg ttccaggctg gtgacctggt tcatgcagcg gctgatcgac
    33901 tggccgatat caatggccgg atagtgcccc gcttccgcca gtttgcgggt taatacgata
    33961 tgcccgtcca gcaccgcgcg cgcgcagtcg acaatcggat cttgctggtc gtcaccttcc
    34021 gccagcacgg tgtagatggc ggtcatcgat cccgcgcttt cgctgttgcc tgcgctttcg
    34081 accagtttgg ggatcatgcc gaatgccgat ggcggataac ctttggtggc gggcggttcg
    34141 ccgagcgaga gggcgatttc ccgctgcgcc atcgcgtagc gggtcaggga atccaccagc
    34201 agtaagacgt gatgcccgcg gtcgcgaaac caggcggcga tggagtggca cagttcggtg
    34261 gctttcagac gcatcagcgg cgattcatcg gcaggcgcga cgacgacgat agactttgcc
    34321 agcccgtccg cgcccagcga gtggtcgata aactctttca cttcgcgccc gcgttcccca
    34381 atcagcccaa ccaccacgat atcggctttg gtctggcggg tgatcatccc cagcagcacg
    34441 cttttcccga cgccgctgcc cgccattagt ccgacgcgct ggcctttgcc gatggtcagc
    34501 aggccgttga tcgctttcac gcccacgtcc agcggttcgt ctaccgaacg acgagttaag
    34561 ggattaaccg agggcggcaa cggcggcaga aggtcgttgc cgttcaggcg gcctttgcca
    34621 tccagcggtt cgccgaggcc attcaccacg cggcctaacc agctttcgcc tatcaggatg
    34681 tcgtgggctt tttcttccgg gaagacgcgt gcgcctgcca tcaggccgac cggctgttta
    34741 aacggcatta aataggtgat gtcgcgatta aagcccacgg cctgcgcgtc gatcagcgtg
    34801 tgattggcac tttcgatgcg gcacagctgc ccggtcatta gcgggcagcc gacactctcc
    34861 agcaggatgc cgttaaggcg caccagccgc ccggcgacgc gggccagcgg gatcgactcc
    34921 agcgagcgca gggcgttatc gaacgcagaa aaatcactca ccctgcgact ccggcgtgag
    34981 tgtttctttc agcgcggtca tgcattgttc aaggcgatgc tcgcagccga tatccagttc
    35041 ggatttgtcg gtgatcaccc ggcattcgcc cggtggcaaa tccggcgaag gcgtcagccc
    35101 ccattcgctg accttttccg gcaccgcgtc gcgcaggcgg ttaaactctt ccgtgctcag
    35161 cattacttgc agggtttccg gcatcgccgg gaaggccgcc agcgcctctt ccaccagcgc
    35221 cagcaactgc gtgggttgca gcgccagttc acaacggatc acctggcggg tgactttttc
    35281 caccagttgc agcaaatctt cccgctgttt acgttcgata tgagcgagga agtcgttcac
    35341 tttaccggtg atggcttcca gcggctgcgc cgcctcaaca aactgtttgc gcccttcctg
    35401 ctgcccggcg aggctgcctt cggtgtaacc ctggcgtcgc ccctcggcaa aaccttgctg
    35461 atgaccttcg ctaaagccct gctcctggcc ttcggtcatt ccttgagcaa accctttttg
    35521 cagcccttcc tgaaatccgt ccatcaactg gcgctggtaa tcggcggcgc taatactggt
    35581 agtgagcggt tctccggtca ccgcacgctg gcgcggcggg aaacggtgca ggcgatagcg
    35641 cccgcgcagt gtttcaatgg tcatggttac tccgccgtct gttccgcaaa gagctgtagc
    35701 tgaatttcgc ccgcctcttc cagttcacgg gcgatgccca tgatttcgcg gcggatctgt
    35761 tcgatacggc tgaccggcac cggccccaga cgcgcggtga tggcttccag ttgctgcgcc
    35821 tgccgttttg gcattaccgc gtaaatcgaa cggcgcagca gggcttcggt gcctttcagc
    35881 gccaccgccc agtcttccat cggcacttcg tcaagcagac gtcgacgcac ctcttcgttc
    35941 tggcggctga ggataaagaa gtcgtacatc tcatcctgaa gctgctccag cacctcttcg
    36001 tcgcgctcgc gcatctggtc gaggatcacc tgctggttgc cctggaagcg gttgacgata
    36061 tccgccgcct gtttgatgcc tttcactttt gagccgtgtt ccgaaagcac cgacaggccg
    36121 cgttcgatca gcctgtccag ttcatccacc acgtcgcggt tgacgtcgtt gagtttcgcc
    36181 acccggtaga gaatttcgtt ttgcaccgat tcgttgagat acgagagcac cgctgccgag
    36241 atttccggcg tcagaaacgc gaggaacacc gcctgtaatt gcagatgttc ttcggagatc
    36301 agcatcgcca gctggcgtgg ttccacccat tgcaggcgcg ccatccgcga gcggatctca
    36361 tcgccgtaga tgccgttgat cacactgctg gcgatctcgg tgccgagggc tttgttgaga
    36421 ataccctgca acatggatcg cgatgcgccg ttgatcccgc tctgttcgcg gaactcatca
    36481 aagaagttat tgatcacttt gcgcgccatg ctggttttca cgccggaaag ccgtgccatg
    36541 ttttcgctca ggcgcaccac ttcttcgcgg ctgagttttt gcatcaccgt cgccgccgcc
    36601 tcttcgccca gacacagtag caggatcgcc gcctgttcca ggtagctgtt gctcttattt
    36661 gttgtcaatt cgctcattgc tggtgatcca ttgtctgaga acttcggcta cgcgatcggt
    36721 gtcgctcatg gccagttttt gcaggaactc gagttttact tccaggccgg aactctgaga
    36781 cggcaggctg tcatcgccgg gcagcgacgg cagatcgacg tttttgcgct cctcttccgg
    36841 catggcaatc cacggttcgc tgacggcttc cgattctggt gtgataatcg gtgcacgtac
    36901 cgccgtcagc cgtttcatta ccgggcgcac cacgaagaac agcaacaaca gcgccagcag
    36961 gccgcagcca atcaggcgca cccaggccag cacgctgtca tctttccaca gcggaataat
    37021 tggttcgacc ggcaccgcct gcggcacaaa gttaagcagt gaaagcgtca ggttatcccc
    37081 acgttgcgcg tcaataccgg cggcattgtt cagcaacgcg gtaagctgcg cggtctgctc
    37141 cggcttccag tttttcagcg ccggagcgtt ttgattgagc accaccgcca cattgaggcg
    37201 tttcaccgca aagccgggat gctgaatatg ctggacgcta cggtcgtaag agtaatcgcg
    37261 cttgctttcg ctgtgtttgg ataacgcttc aggcgagcga ttttcttccg taccgttagt
    37321 catttgattc gccgcaaccg gcggacggtt gctgagagag ccgggcacac ccatcgccac
    37381 ctgattggtg tcgctgtcca gcacgctctc ttcgcggttt attttcggcg cgtcgccgta
    37441 gtgttcctga gtttcgtcga tggtgctgag gtcgagatcc ggcatcacgc tgacacggta
    37501 attcccgctg ccgaccagcg aatccagcac gttcgccacg ctggcgtggg ttttgtcctg
    37561 aatgtctttg aggatctgat cgcgtttacg ggtagcggca gagaccgcct cgcccgcgcc
    37621 gatgccgtcg gtcagcagat ttcccgcctg atcaaccacg ctaactttgc tggcgtgcag
    37681 ccccggaatg ctgccggaaa ccagatgcac aatggcgttc acctggtcca tattcagctt
    37741 cgcgccgtag tgcagacgca ccaccaccga ggcgctgttt tgcggctcat cgctgaccac
    37801 aaacgagctt tcttcgttga gcgccagatg cacccgcgcg ctctccaccg cgtccagcga
    37861 cataatgctc tgcgccagct ccctttcgag gctgcgttta tagcgcacgt tctgcacgaa
    37921 ctggctgctg ccaagcactt cgtctttgtc catcagctcg tagccgctgg gcaaaatcgc
    37981 ctgcacgcct ttggcggcaa gggtcatgcg cgttttcgac agcgcctctt ccggcaccag
    38041 tatctgcccg ctttgcgggt caatgcggta ggtgagcttt tcgccgtcca gcacggtgac
    38101 aatctgcgac acgggcagat tttcctggcg tccgtagagc gagacgtagc cgcgattgcc
    38161 ggtccacagc acgctaacga taatcgccgt cgccgccacg gcaagcccca cacccgccat
    38221 cagcgcccag cgtttgttat ctgcgagacg cagtcgaaac gccggaaacg ccagggttaa
    38281 cttctttatt tgtgcattca tagggttaga tcgacatact catcagatcg ttaaatccgg
    38341 tggcgatttt gttgcgcacc tgcaccagcg cggagaaaga gagcgacgcc tgctggctgg
    38401 cgatcatcgc acctgccaga tcatcgcttt tgcccatgtc gatagccgtt tgctgctgct
    38461 ccgccacctg ctggaactgg tcaacgtgac cgagcgcgcc ctgcataatg cggtgaaacg
    38521 aaacgtccgg gtcggtggaa ctgaaggtca tggcgggcag caccggcgcc tgggcgttgg
    38581 cctgcatccg ctgcacgtcc tgcatgatct gcgcctgcat ggtgctgttg attgtggtaa
    38641 tgctcatttt taacttccgg gtcaggagaa aacttcgata ccgtgcttgc gcatggaggc
    38701 cagtcgatag cgcagtgcgc gcggggtaat acctaacagg tcggcgattt tgctgcggtt
    38761 gccctgatat ttacgcatca ggtcagcgat atattgatac tgggcgcttc gtccgtgctg
    38821 gcctaaatcg ctggtttcgg caatgtgtac ggcgggctga gcaggccatt gcagttcgct
    38881 gtggtcggca atgtcatttt ccggcaggcc taaggcgtcc gggtaaatca cgccgtcgcg
    38941 atttaaaatc attccccgct gaatggcgtt ctccagctgg cgcacattgc cgggccagcg
    39001 gtaattcagc atcgcacggc gggtagattc tgaaagggtg atatttttca ccagcaccgt
    39061 ggagtatttt tttataaatg actctgccag cggaataata tcgttcagac gttcacgcag
    39121 cggcggcatg gtaataggaa taaccgccag acgataatag agatcttcac gaaaacgccc
    39181 ggcggcgact tcctgctcaa ggtttttatt ggtgcaggca atcaggcgaa aattgagttt
    39241 aatttgtcgg ttgctgccta atcgctcaac ctgttgttcc tgcaatacgc gtaatatttt
    39301 ggcctgtaac gccagcggca tatcgccaat ttcatcgagt aataaggtgc cgttattcgc
    39361 cagttccatt tttcctggca cactggcaat tgcgccggta aatgcgcctt tgtcgtagcc
    39421 aaataaggtt gcttccagca tgttttccgg gatcgcagcg cagttcacgc cgatataggg
    39481 cgcgttttca ttttcaccaa aggcgactgt gtgaatatat ttcgccacgc actctttgcc
    39541 cgcgccggtt tcgccctgaa tgagcaccgg cacgttaaat gcggcgacac gctttgccag
    39601 agtaaaagcg ttaatgctgg aagcggcagt tgcaataagt tcagacatag ggtgaacgat
    39661 ataagtagcg acaataatca ggatgttctg aagcgtatca ccggcgggat taacaagttc
    39721 gaattaaacg ataataaaaa cattatttgt ttataaacaa tgatttatcc atgtaggccg
    39781 gataaggcgt ttacgccgca tccggcaatt gtgcaccggt gcctgatgcg atgcggcgcg
    39841 tcttatcagg cctacggttc gtgcaatgtg ttatgacgat gcctgatatc tcgcccttta
    39901 ataatttaat tattttttca ccctattaat aatttgcccc tttcacgccc cctctattta
    39961 atacctcacc ttgtgtcact gccggatttg cgtgcacaaa atcgcccata ccattacagc
    40021 agagaaagta agccatgctg aagtacagta aaacgccagg catctttaaa ttagaaggta
    40081 atcggctcgg acgcccctac catcgcctgc cgaccctgtt tacagggaat tttgacgtga
    40141 ttgattcgca tctgggcagc tactttctca aaaagcatcg cagcaatatc accctaaaaa
    40201 agattggctg cgaaatggat attattaata aaaacgctga actgatggtt tcccaggtcg
    40261 ggcatctggc gtttgatatt gatcgctctt tgctgttaat gctgctgggc aatttttatg
    40321 gtctggagtc ctctcttgaa gaggcgaaag cgcataacgg gctgccgacc aaaaccgaaa
    40381 cgcggttaaa aaaccgtctg gcgctggata tctgcacgct gatttttaat ctgcaaacct
    40441 ccggtattgc gctgaaatta aagctggaca gcagcacggt aattacccac tgggcgtatc
    40501 aattaacctt tacccttgcg ggcgatgaag agagctgttt tcgtattttg ctcgacgacg
    40561 cccacaccga ttttatttta aatctgatcc gccacagcga gcacagtaaa ccgcagcagg
    40621 tggcgaaatc ggtcaataaa ccggccctga ttaaagagat catccgctcc ctgccgctga
    40681 ccctgaacgt caaaatcgcc gagctatcga tgaatgtcgc ggatctcacg cagataaaag
    40741 ccggggacat tttacccatc tcgctgggag aaacgttccc ggtggcgatt gggcaatctg
    40801 aattatttag cgccctgatt gtggaagaca aagacaaact gtttttgtcg gagctggcgg
    40861 gcaaaaatga gaatagccat gagtaagcaa gaagatattt tagccgggga ttttggcctc
    40921 accgatgaag ttgcgcctgt cgcaaaatcc gccgacgctg aaacgctggt cacccgcctg
    40981 gaagaccgtt tttccgactc gatgacgctg ctaaaacgca ttccggtgac cttaacgctg
    41041 gaagtgtcat cagtagaaat aatgttagcc gacctcctta acatcgacga cgacacggtg
    41101 attgagctgg ataaactcgc cggggagccg ctggatatca aagtgaacaa tatcctgctg
    41161 ggcaaagcgg aagtggtggt ggtcaacgag aagtacggcc tgcgcgtgct ggaatttaac
    41221 acccgcgata tcaacgacct ggcaccatga aacgccgcac tcaactgacg ctgggccttg
    41281 ggctgctggc gcttgcgccg gtggcgttgg cccagggcgg cgatattgcg ctgcttaacg
    41341 tggtcaccca cggcaacact caggagtaca gcgtcaaaat ccaggtgctg attctgatga
    41401 ccctcgtcgg tttgctgcct acgatggtgc tgatgatgac ctgctttacg cgctttatta
    41461 tcgtgctgtc gctgctgcgc caggcgctag ggctacaaca aacgccgcca aaccgcattc
    41521 tgattggcat tgcgctctcc ctcaccatgc tggtgatgcg ccccatctgg ctcaatattt
    41581 acgaccacgc agtagtgccg tttgaaaatg atcagattac cctgaccgat gcgttaagca
    41641 ccgccgcgac gccactcaaa cgctttatgc tggcgcagac tgacaaaaaa gcgatggcgc
    41701 aaatcatgac tatcggcggc gcgaagggcc atgccgccga ccaggatctc tcgattgtgg
    41761 tgcccgccta tgtgttgagc gagctgaaaa ccgccttcca gattggcttt atgatttaca
    41821 tcccgtttct ggttatcgac ctgattgtcg ccagcgtact gatggcgatg gggatgatga
    41881 tgctgtcgcc gctgatcgtt tcgctaccct ttaagctgat gctgtttgtg ctgatcgacg
    41941 gctggtcgct gaccatcggt acactcacca ccagcattcg cgggctgggg ctgggctgat
    42001 gatgctgacc atagacgtag ccgccgacat tgtcgccagc ggcataaaag tggtgatttt
    42061 gctggtttcg gtgctggtag tgccgagcct gctggtcggc ctgctggtga gcgtgttcca
    42121 ggcggtaacg cagattaacg aacagaccct aagctttttg ccgaggctga ttgtcacgct
    42181 ggtggtgctg ggcgtgtgcg gtaaatggat gattatccag ttgcatgatc tctgcattca
    42241 cctgtttacc caggccgcgt tgctggttca ttgagatgcg cacaagcgac gttacgcaac
    42301 tgatggatct ggcgctgggg ctgtggtttc cctttgtgcg cattatggcg tttctgcgct
    42361 atgtgcctgt gctggataac agcgccctga ccgtgcgcgt gcggattatt ttgtcgctgg
    42421 cgctggcaat catcatcacg ccgttgatcc cccatcctgt tccccacgac ctgatatcgc
    42481 tcaacagcct gatcctgacg gtggagcaaa ttttatgggg catgttattc gggctgatgt
    42541 ttcagttttt gtttctggcg ctacagctgg ccgggcaaat tctctcgttc aatatgggga
    42601 tgagcatggc ggtgatgaac gacccgagca gtggcgcatc gaccaccgtg ctggcggagc
    42661 tcatcaacgt ctacgcgatc ctgctgtttt ttgccatgga cgggcatctg ctgctggtga
    42721 gtgtgctgta taagggcttt acctactggc cgattggcaa cgcgctgcat ccgcaaaccc
    42781 tgcgcaccat cgcgctggcc tttagctggg tgctggcgtc cgcatcgctg caggcgctgc
    42841 caaccacttt tatcatgctg attgtgcagg gctgctttgg cctgctcaac cgcattgcgc
    42901 cgccgctgaa ccttttttca ctcggttttc ccatcaacat gctggcgggg ctggtctgct
    42961 ttgccaccct gctctacaac ctgccggatc actatctgca tctggcgaat tttgtgttgc
    43021 agcaactcga cgcgctgaaa ggtcactatg gcggatagca gcagcgaaga aaaaacagag
    43081 aaaccctcgg cgcaaaagct gcgtaaagcg cgcgaagagg ggcaactgcc gcgctcgaag
    43141 gatatggggc tggcggcgag cctgtttgcg gcatttgtgg tgatctccag cagcttcccg
    43201 tggtatgccg attttgtgcg cgagagtttt atcagcgtgc accagtatgc acaggagatt
    43261 aataacccag aggtgatcgg ccagtttctg cgccatcacc tgttaattct cggcaagttt
    43321 attctcacct tgctgccgat gcccgccgcc gcgctactct cctcactagt gcccggcggc
    43381 tggctgtttc tgcctaaaaa aatcctcccg gatttcagca aaatcagccc gttaaaaggc
    43441 attgagcggc tgttttccag tgaacatctg gcggaaacgg gaaagatgac ggtgaagtcg
    43501 gtggtggtgc tggtgatgct gtgggtaagc ctgcgcaata actttgcctc ctttctcggc
    43561 ctgcaggcgc taccgttcaa actggcgatg aatgagggtt tatcgctcta cgcctgcgtg
    43621 atgcgcaact ttgtcatcct gtttatcttc tttgcgttaa tcgacgtgcc gctggcgaaa
    43681 gccctgttta ccaaagggct gaagatgacc aaacaggagc tgaaagaaga gtataaaaac
    43741 caggaaggca agccggaagt gaaggcgcgc gtgcggcgtc tgcaacggca gttggcgatg
    43801 gggcaaattc gcaaagtggt gccgaaagcc aacgtggtga tcaccaaccc gacccactac
    43861 gccgtggcgt tgcagtatga ctcgtcgcgc gccgccgcgc cgtttgtggt ggcaaaaggc
    43921 accgatgaaa tcgccctcta tattcgccag gtcgccgctg agaatcaggt ggaagtggtc
    43981 gagttcccgc gactggcgcg ctcggtttac tacaccaccc aggtgaacca gcaaattccg
    44041 tttcagcttt accgggccat cgcccacgtt ctgacctacg tcctgcaaat gaaacactgg
    44101 cgcgagggtg cacagccgcg cccggccctg aacagacata tttccattcc caaagaggtg
    44161 cttaaactgg atggcgaaaa caactaaatc gttcctcgcg ctgctgcgcg gcggcaatct
    44221 tggcgtgccg ctggtgatcc tgtgtattct ggcgatggtt attttgccgc tgccgcctgc
    44281 gctgctggat attctgttca ccttcaacat tgtgctggcg gtgatggtgc tgctggtggc
    44341 ggtgtcggcg aaacgaccgc tggaattcag cctgttcccg accattttgc tgatcaccac
    44401 cttaatgcgc ctgacgctca acgtcgcctc tacgcgcgtg gtgctgctgc acgggcatct
    44461 cggcgcggac gcggcgggta aggtgattga gtcgtttggt caggtggtga tcggcggcaa
    44521 ctttgttgtc ggctttgtgg tgtttatcat cctgatgatc atcaacttta ttgtcgtcac
    44581 caaaggggcc gagcgtattt ccgaggtttc agcccgcttt accctggatg cgatgcccgg
    44641 taagcagatg gcgattgacg ccgatcttaa tgccgggttg atcaatcagg cgcaggcaca
    44701 aacccgacgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
    44761 gaaatttgtg cgcggggatg ctatcgccgg gatgatgatt ctggcgatca acctgatcgg
    44821 cggcgtctgt atcgggatct tcaaatacaa cctgagcgcc gacgccgcct tccagcagta
    44881 tgtgctgatg accatcggcg acgggctggt ggcgcaaatc ccttccctgc tactctccac
    44941 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
    45001 tcaccagttg ctggcaagcc catcggtgct ctacaccgct accggaatta tgttcgtact
    45061 ggcggtagtg cctggaatgc cacatctgcc gtttttgctg ttcagcgccc tgcttggctt
    45121 taccggctgg cggatgagca aacgcccgca ggcggcggaa gcggaagaga aaagtctcga
    45181 aacgctgacc cgcaccatca ccgaaaccag cgagcaacag gtcagttggg aaaccattcc
    45241 gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg tggacaaagc
    45301 ccagggcaac ccgctcaccc agcgtattcg cggcgtgcga caggtgatat ccgacggcaa
    45361 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgccttaagc ccagccagta
    45421 cgctattttc atcaacggca ttaaggctga tgaagcggat attccggcgg ataaactgat
    45481 ggcgctgccc tccagcgaaa cctacggcga gatagacggc gtgctgggga acgacccggc
    45541 gtacgggatg ccggtgacct ggatccagcc tgcgcagaaa gcgaaggcgc tgaatatggg
    45601 gtatcaggtg atcgacagcg ccagcgtgat tgctacccat gtaaataaga ttgtgcgcag
    45661 ctatattcct gatttattta actatgacga tattacgcag ttgcataacc gtttggcgtc
    45721 gatggcaccg cgcctggcgg aggatttaag cgcggtgctc aattacagcc agttgctgaa
    45781 agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
    45841 cgtgctggtc gccagtagcg cggtgaccaa agatcatatt ctgctggcag ccgatgtgcg
    45901 cctggcgctg cggcgcagca ttacccatcc gttcgttcgc aagcaggagc tgacggtgta
    45961 taccctgaat aatgagctgg aaaatctgct gactaaccta gtgaatcagg cgcaacaggg
    46021 cggtaaagtg atgctcgaca gcgtgccagt ggacccgaat atgctcaacc agttccagag
    46081 tacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagacccgg tgctgctggt
    46141 gccaccgcag ctgcgccctt tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca
    46201 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg gggcgttgag
    46261 ttgatggggg attcttttcc atgctttgca gcacctgaca ccattaattg acgtcacact
    46321 gcaaagagac tacgatgaca ccatacaatg acgtcatgga ttgatagtat gattaaggtc
    46381 agcgcgctag gttctcgtca tagaaaaaca ttcgacgatg taatgacgga gccgccaaaa
    46441 tctggaatta aatgggatga tgttgttgca ctcattaaag ctgtgggtgg aacaataaaa
    46501 aataacaacg gctccagacg aaaatttcag attggaacga ccaaattcag cacacatgag
    46561 ccacatccaa aaaatgtgat ggataaaggc gcagtagcag gtcttaagga gtggtttgtt
    46621 aactgtgtag gggtagatta tgaataaagc atcgaacatt atcacaatta aaggtcaacc
    46681 agccgcagta acatttgaag ccgatatcaa cgcatttcgc ggcaagttcc tcaatgtaaa
    46741 cggttattgt gactttgtag ccacaagtat tgaggcatta tatcgtgaag gcgaaagcgc
    46801 gctggatgag tggatggcgg attgtgaaga ggacggtatt gccccttttc gtgaagaaga
    46861 agagcaaaaa cgcctgacgc ttcgtgttcc tcatcacatt gattcgcggc tgaccatcgt
    46921 cgccagacaa cattccattt caaaaaacca gctcattgtt gatgtcttag aacgtgagtt
    46981 ttcagcccag atgtaaacag gtcggatgcc gcgtggcatc cgaccttttg ccagttcgtc
    47041 aatattaccc atttacaaaa tcgtctcctc cgtcaaaatc cgccgcgcgc ctaaaaagtg
    47101 atcctgccag aaaggttcag ctaactgact tacccgaatg gtttccccgg tgcgcggtga
    47161 ctcaataaac tgaccgtcgc ccagatacac ccccatatgg tcggcaatct cgcggctgtg
    47221 gatatggaaa aatagcaaat ctccccggcg caggtcgtta ttcgccacaa tggttgcccg
    47281 gcgatagtga tacatctcat tagccgtgcg tgggagtttc gcctcaagga tcttgttgta
    47341 ggcataaaaa actaacccgc tacagtcgaa gccttgatcg ggccgcgtac cgccccagac
    47401 gtaaggcttg ccaagctgtt gctccagacg gtgaatcgcc acttcggtga tgttatgcag
    47461 atggtcgctg cttaagaaat ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc
    47521 cttgagcggc gcaggaaacc attctggatg ctgttttatc tgttcgcggt tgtgctggcg
    47581 tagcgccctt ctgctttcgg cattgccttc cacaatatag ctggcctgtt ttttcagatg
    47641 ggtttggtac tgttttaata aacgcgcacg attttgcatc cgctggcggg cggcgtagct
    47701 gaaagtgata tgtgatgtgg tggcgctggc aaatgaagaa aacggcaagc aaagcaacag
    47761 taatcccgac aacacaaaag agggaatcga cggcaaggac atactcaata atggctgatc
    47821 atgcattgat gttgcagtgt atcccggccc gtacctgaac aaacttaata aaccattatt
    47881 ataaaattaa taaatatatt tcagttaatt atgtaaaaac atccgtttat taaattactc
    47941 atggattgtc gctgttgctc atttgagcta caattcaagc tgaataaata tacagcacag
    48001 gagatacccc aatggctgct aacgcatttg ttcgcgcccg aatcgatgaa gatctgaaga
    48061 atcaggcggc ggacgtactg gccgggatgg ggctgaccat ctctgacctg gttcgcataa
    48121 ccctcacaaa ggtcgcgcgt gaaaaggcat tgccgtttga tttacgcgag cctaatcaat
    48181 taaccattca atcaatcaaa aacagcgaag ctggcgttga tgttcataag gccaaagacg
    48241 ccgatgattt atttgataaa ttaggaattt aaatcaaagg gatattgaat actcgggaca
    48301 attttcaaag gatgtaaaac ttgcacaaaa gcgtcataag gatatgaata aattgaaata
    48361 tcttatgacg cttcttatca ataatgcttt accgcttcca gctgtttata aagaccaccc
    48421 gctgcaaggt tcatggaaag gttatcgcga tgctcatgtc gaaccggact ggatcctgat
    48481 ttacaaactt accgataaac ttttacgatt tgagagaact ggaactcacg cggcgctctt
    48541 tgggtaaagc acgctgccga tgaaatttgt aaaccctgcg ccgacaggct tgcgtaaaac
    48601 tcttttggtg ataatgtgag ccttccaaat ccagactatt ccgatttggg atctattttg
    48661 ccgtcctggc gtgtgtaacc gttttatcaa ggaataagca gtatgcgtaa aatcgcatta
    48721 attcttgcga tgttgttgat cccgtgcgtt tcgtttgccg gtttgctggg cagcagtagc
    48781 tcgaccacgc cagtgagcaa agagtataag cagcagttga tgggatcccc tgtctacatc
    48841 cagatcttca aggaagaacg tacgctcgat ctctacgtca aaatgggcga gcaatatcag
    48901 ctgctcgaca gttataaaat ctgtaaatat tccggcggct tagggccaaa acagcgtcag
    48961 ggcgatttca aaagcccgga agggttttat agcgtccagc gtaatcagtt aaaaccagac
    49021 agccgttact acaaagcgat taatattggt ttccccaatg cctatgaccg tgcgcatggt
    49081 tatgaaggga aatacctgat gatccacggc gattgtgttt ccatcggctg ctacgcaatg
    49141 accaatcagg gtattgatga gatcttccag ttcgttactg gtgcgctggt gtttggtcag
    49201 ccgagcgtgc aagtgagtat ttacccgttc cgcatgaccg acgccaatat gaagcgccat
    49261 aaatattcca actttaagga cttctgggag cagctgaagc cgggctacga ctactttgag
    49321 caaacccgta agccaccgac cgtttctgtg gtcaatggcc gttacgtggt cagcaagccg
    49381 ttaagccacg aagttgtgca gccacaactg gcatcaaact acacgctccc cgaggcaaaa
    49441 taagcgccat tcgcctggca taatcttttg ccaggtttca ttgcccgtca gcggctgtgt
    49501 tgcaatcacc gtgaccacat cattcggtgt ggtctgcgag ctgaagtcga tttccacatc
    49561 ctgatccagc agcgttgcca cgccaaacgg cgcgcggcgg gtgatccagt gtaaattagt
    49621 cgagcaatac gccatcacat agcgcccgtc agaaagcagc atgttgaaaa cgcccttctg
    49681 ccgcagttca tccgccagtg aggcgatata tttaaacact gccgccatgt tgcccggtgt
    49741 gcgcgggtaa cgctgcgtta atttatgcag gagccagcag aaggcttttt cgctatcggt
    49801 ttcaccgacc gggcggaagt tgccggtttc cagtgatttg tagcccgtca gttgtccgtt
    49861 atgggcataa gtccagttac gcccccataa ctcgcgggta aatgggtgag tattttccag
    49921 cgccacctcg ccccgattag cctggcgaat atgagccacc accgaacagg attttatcgg
    49981 gtagtcctgg acaagtttgg cgatggggga attaaagctg ggttgtggat ctttaaatgt
    50041 gcgacagcct ttaccttcgt aaaaggtaat gccccagcca tctttatgtg gcccggttcc
    50101 accaccacgc tgtacaagtc cggtgaaact aaagcagata tcggtaggga cgttggcgct
    50161 catcccgagc aattcgcaca tgggtaacct ccacaacagc gggagcgaat ggcccccggc
    50221 gaagattgat ttaagaattt tcaggtcgga tgcggcgtaa acgaattatt cggcctacgg
    50281 ttcgggtaca gatttgtagg catgataaga cgcgtcagca tcgcatcagg catcagcgca
    50341 caagtgccgg atgcggcgta aacgccttat ccagcctacg ccagacttac ttaaccatct
    50401 ctttttcaat taactggatc aggatatgga tcactttaat gtgaatctcc tgaatgcggt
    50461 cggcataacc aaagtgcggt acgcgaattt cgatatccgc cgtgccagcc attttgccgc
    50521 cgtctttacc ggtcagggtg atcactttca tccccttctc acgcgccgct gcgatcgctt
    50581 tgatcacgtt tgcagagtta ccggaggtgg agatccccag cagtacatcg ccttcgcgac
    50641 ctaccgcttc aacgtagcgg gagaaaatat cattgaaacc gaaatcatta ccgacgcagg
    50701 aaatatgact aacgtcagaa atagcaatcg ccgggtagcc cggacggttt tcacggtagc
    50761 gaccggtcaa ctcttcggca aagtgcatag cgtcgcaatg ggaaccgccg ttgccgcagg
    50821 aaagcacttt gccaccggct ttaaagctgt ctgctaacag gaccgccgcg cgctgaatgg
    50881 cgtgaatatt ggcgtcatct tttaaaaagt tagccagcgt ttccgccgct tcgttcagtt
    50941 cgttacgaat aagatcctgg tacatgagga tatccttcag cataaatgta atagacaaaa
    51001 tgcagtgtac cggataccgc caaaagcgag aagtacgggc aggtgctatg accaggactt
    51061 tttgacctga agtgcggata aaaacagcaa caatgtgagc tttgttgtaa ttatattgta
    51121 aacacattgc taaatgtttt tacatccact acaaccatat catcacaagt ggtcagacct
    51181 cctacaagta aggggctttt cgttatgatg attttgagta ttctcgctac ggttgtcctg
    51241 ctcggcgcgt tgttctatca ccgcgtgagc ttatttatca gcagtctgat tttgctcgcc
    51301 tggacagcct ccctcggcgt tgctggtctg tggtcggcgt gggtactggt gcctctggcc
    51361 attatcctcg tgccatttaa ctttgcgcct atgcgtaagt cgatgatttc cgcgccggta
    51421 tttcgcggtt tccgtaaggt gatgccgccg atgtcgcgca ctgagaaaga agcgattgat
    51481 gcgggcacca cctggtggga gggcgacttg ttccagggca agccggactg gaaaaagctg
    51541 cataactatc cgcagccgcg cctgaccgcc gaagagcaag cgtttctcga cggcccggta
    51601 gaagaagcct gccggatggc gaatgatttc cagatcaccc atgagctggc ggatctgccg
    51661 ccggagttgt gggcgtacct taaagagcat cgtttcttcg cgatgatcat caaaaaagag
    51721 tacggcgggc tggagttctc ggcttatgcc cagtctcgcg tgctgcaaaa actctccggc
    51781 gtgagcggga tcctggcgat taccgtcggc gtgccaaact cattaggccc gggcgaactg
    51841 ttgcaacatt acggcactga cgagcagaaa gatcactatc tgccgcgtct ggcgcgtggt
    51901 caggagatcc cctgctttgc actgaccagc ccggaagcgg gttccgatgc gggcgcgatt
    51961 ccggacaccg ggattgtctg catgggcgaa tggcagggcc agcaggtgct ggggatgcgt
    52021 ctgacctgga acaaacgcta cattacgctg gcaccgattg cgactgtgct tgggctggcg
    52081 tttaaactct ccgacccgga aaagctgctg ggtggcgcag aagatttagg tattacctgt
    52141 gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt cccgctgaac
    52201 gtaccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat cgattacatc
    52261 atcggtgggc cgaaaatggc cgggcaaggc tggcggatgc tggtggagtg cctctcggta
    52321 ggccgcggca tcaccctgcc ttccaactca accggcggcg tgaaatcggt agcgctggca
    52381 acgggcgcgt atgctcacat tcgccgtcag ttcaaaatct ctatcggtaa gatggaaggg
    52441 attgaagagc cgctggcgcg tattgccggt aatgcctacg tgatggatgc tgcggcatcg
    52501 ctgattacct acggcattat gctcggcgaa aaacctgccg tgctgtcggc tatcgttaag
    52561 tatcactgta cccaccgcgg gcagcagtcg attattgatg cgatggatat cactggtggc
    52621 aaaggcatta tgctcgggca gagcaacttc ctggcgcgtg cttaccaggg cgcaccgatt
    52681 gccatcaccg ttgaaggggc caacattctg acccgcagca tgatgatctt cggacaagga
    52741 gcgattcgtt gccatccgta cgtgctggaa gagatggaag cagcgaagaa caatgacgtc
    52801 aacgcgttcg ataaactgtt gttcaaacat atcggtcacg tcggtagcaa caaagttcgc
    52861 agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg cgatgccact
    52921 aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc
    52981 tcgatggctg tgctgggcgg cagcctgaaa cgtcgtgagc gcatctcggc acgtctgggg
    53041 gatattttaa gccagcttta cctcgcctct gccgttctga agcgttatga cgacgaaggc
    53101 cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac aagatgcgct gtatcaggct
    53161 gaacaggcga tggatgattt actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg
    53221 aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa gctggatcat
    53281 aaagtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg tcgcggtcag
    53341 tacctgacgc cgagcgagca taatccggtt ggcttgctgg aagaggcgct ggtggatgtg
    53401 attgccgccg acccaattca tcagcggatc tgtaaagagc tgggtaaaaa cctgccgttt
    53461 acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga taaagatgaa
    53521 gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt tgatgacttt
    53581 gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg gaaagttgaa
    53641 gccgcgtaat tgaataacgg agccgaaagg ctccgtttct ttatccgcta attatttaaa
    53701 attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca gcaccgtttt
    53761 accatcaatc gaaagcgcat cgttcacatt cagccaggtg agtttctctt gcgacgtttt
    53821 ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc acatcacagc
    53881 gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat acgtctcatc
    53941 tcccaacgtt acggtttgtg cgggagtata agtaccgcct ttcatcaccc aggcaggcag
    54001 cttatgccct tgtaccatct gattaaatgc agctttggtg gtttcgccct ttgcaaggct
    54061 gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag cggcgactgt
    54121 cgttattgcc ttaaacatca ttcctcccga gcttatcctg cccatgttat taaccttcta
    54181 aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag catttgttat
    54241 caatttattc tgcgctaagg caactgcctg ataaggtgtt aaagttataa aacccttcgc
    54301 ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca accactggtg
    54361 tggatggatg gccctgccaa cctgcgtcat tttgatcgtc aactggaagg tattaccggg
    54421 cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga agcggcagct
    54481 tcgtcgctag cacaagatga cgtagtgaac tggatgacag ctaaggcgca gcagtgcaat
    54541 gcgctgattg caggcagtgt tgcattacaa acggagtctg gttcggttaa ccgctttttg
    54601 ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt ccgcatggca
    54661 gatgagcatc tacattataa agcgggcaat gcgcgagtga ttgtggaatg gcgcggctgg
    54721 cgtattttgc cgctggtgtg ctacgactta cgttttcctg tgtggtcgcg caatctcaac
    54781 gattatgacc tcgccctgta cgtcgccaac tggcctgctc cgcgctctct gcactggcag
    54841 gcattgctga cggcccgcgc gattgagaat caggcgtatg tggcgggatg caatcgcgtc
    54901 ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa tccacaaggc
    54961 gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgccga gctgtcgatg
    55021 gcggtgttaa gggaatatcg cgataagttt ccggcatggc gggatgcgga tgagtttagg
    55081 ttgtagtgag tgggggctgt atttagggcg gggaattcag aaagggatgt ttagtgtgct
    55141 gagcgagagt taaataataa aaaactggga agatttaata atccccccgc agggcaagag
    55201 catgaattaa atttgtgtta aatttttcat tttatcagtt cgaaacccaa gctctgagca
    55261 ggatgcaagc acttcttcta tataacatgt gtttaacgcc agggcaagat tacgaaagcc
    55321 cgctccccgc aaggactgac gccagatagt ttctgtccat ggctgctttt cgcatcttac
    55381 gtcttaaccc tgccttgaat accttatcat tcgtcaaaat attaatagca atgtgccgta
    55441 tccctgaaaa taattctgct gcatttcctc ttcttatttt gcagtcgtct tcattcatta
    55501 ccacgtccag acgccagtgc agcttattct ccacgtgcca gtgatttcgg atcgctgtgg
    55561 cgaatttctc agcggttaaa tcagcagaac tgatataata tctgaccgtc atttctggct
    55621 ctttcttttg ttctgctatt atggaccgaa aggagactgc cacgcataat ttcttcagcc
    55681 ctttccattc aaacgtgaaa tcaataagtt catcagggac atcgcaaaca atatgaagac
    55741 ggatttcttc tctgccgtga ctcttttcac taattgcgta actgtcatgc tctggattat
    55801 ttaattcttt cagcggaaat ttttcctcaa aggctttatt tagccgcccc tggtttcctt
    55861 ttaccgcgaa taaataatca cctccctgtt tttgtatctt ctctgcaata tctttctggc
    55921 aacccatcgc atcagttgtg atgatttttc ctttaatatc cagcatgtta agaagttctg
    55981 ggatagctgt aatctcatta gatttctcat ccgtcctgat ctgtccgatg accagactgt
    56041 gcattgttga gaacgcacta atgacatgaa tcgctcccct gcggcgactc ttgtcataag
    56101 agtgccggag cgtttttcca tcaattgcga tgacgtcttt atcatctgaa gaatggcagt
    56161 cacgcatcca gttaataaag cactcatgaa attttgcagg actgatacag gatacaactc
    56221 tggcaatagt atcgtgaaca ggaata
//
LOCUS       DAZFDP010000014        54595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFDP010000014 DAZFDP010000000
VERSION     DAZFDP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            303..389
                     /gene="leuL"
                     /locus_tag="ACHJ1G_000946"
     CDS             303..389
                     /gene="leuL"
                     /locus_tag="ACHJ1G_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4762844.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            482..2053
                     /gene="leuA"
                     /locus_tag="ACHJ1G_000947"
     CDS             482..2053
                     /gene="leuA"
                     /locus_tag="ACHJ1G_000947"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4762845.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2053..3144
                     /gene="leuB"
                     /locus_tag="ACHJ1G_000948"
     CDS             2053..3144
                     /gene="leuB"
                     /locus_tag="ACHJ1G_000948"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4762846.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3147..4547
                     /gene="leuC"
                     /locus_tag="ACHJ1G_000949"
     CDS             3147..4547
                     /gene="leuC"
                     /locus_tag="ACHJ1G_000949"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4762847.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4558..5163
                     /gene="leuD"
                     /locus_tag="ACHJ1G_000950"
     CDS             4558..5163
                     /gene="leuD"
                     /locus_tag="ACHJ1G_000950"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4762848.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5212..6390)
                     /gene="setA"
                     /locus_tag="ACHJ1G_000951"
     CDS             complement(5212..6390)
                     /gene="setA"
                     /locus_tag="ACHJ1G_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO4762849.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="ACHJ1G_000952"
     CDS             complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="ACHJ1G_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4762850.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6712..8367
                     /gene="sgrR"
                     /locus_tag="ACHJ1G_000953"
     CDS             6712..8367
                     /gene="sgrR"
                     /locus_tag="ACHJ1G_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4762851.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8401..8500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8531..9514
                     /gene="thiB"
                     /locus_tag="ACHJ1G_000954"
     CDS             8531..9514
                     /gene="thiB"
                     /locus_tag="ACHJ1G_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4762852.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDLRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9490..11100
                     /gene="thiP"
                     /locus_tag="ACHJ1G_000955"
     CDS             9490..11100
                     /gene="thiP"
                     /locus_tag="ACHJ1G_000955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4762853.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11084..11782
                     /gene="thiQ"
                     /locus_tag="ACHJ1G_000956"
     CDS             11084..11782
                     /gene="thiQ"
                     /locus_tag="ACHJ1G_000956"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4762854.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11896..12660)
                     /gene="yabI"
                     /locus_tag="ACHJ1G_000957"
     CDS             complement(11896..12660)
                     /gene="yabI"
                     /locus_tag="ACHJ1G_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4762855.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12746..13624)
                     /gene="araC"
                     /locus_tag="ACHJ1G_000958"
     CDS             complement(12746..13624)
                     /gene="araC"
                     /locus_tag="ACHJ1G_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4762856.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13963..15663
                     /gene="araB"
                     /locus_tag="ACHJ1G_000959"
     CDS             13963..15663
                     /gene="araB"
                     /locus_tag="ACHJ1G_000959"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4762857.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15674..17176
                     /gene="araA"
                     /locus_tag="ACHJ1G_000960"
     CDS             15674..17176
                     /gene="araA"
                     /locus_tag="ACHJ1G_000960"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4762858.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17345..18040
                     /gene="araD"
                     /locus_tag="ACHJ1G_000961"
     CDS             17345..18040
                     /gene="araD"
                     /locus_tag="ACHJ1G_000961"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4762859.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18115..20466
                     /gene="polB"
                     /locus_tag="ACHJ1G_000962"
     CDS             18115..20466
                     /gene="polB"
                     /locus_tag="ACHJ1G_000962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4762860.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20631..23537
                     /gene="rapA"
                     /locus_tag="ACHJ1G_000963"
     CDS             20631..23537
                     /gene="rapA"
                     /locus_tag="ACHJ1G_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4762861.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23549..24208
                     /gene="rluA"
                     /locus_tag="ACHJ1G_000964"
     CDS             23549..24208
                     /gene="rluA"
                     /locus_tag="ACHJ1G_000964"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4762862.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="ACHJ1G_000965"
     CDS             complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="ACHJ1G_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4762863.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25395..27749
                     /gene="lptD"
                     /locus_tag="ACHJ1G_000966"
     CDS             25395..27749
                     /gene="lptD"
                     /locus_tag="ACHJ1G_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4762864.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEPPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKHYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27802..29088
                     /gene="surA"
                     /locus_tag="ACHJ1G_000967"
     CDS             27802..29088
                     /gene="surA"
                     /locus_tag="ACHJ1G_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4762865.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29088..30077
                     /gene="pdxA"
                     /locus_tag="ACHJ1G_000968"
     CDS             29088..30077
                     /gene="pdxA"
                     /locus_tag="ACHJ1G_000968"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4762866.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30074..30895
                     /gene="rsmA"
                     /locus_tag="ACHJ1G_000969"
     CDS             30074..30895
                     /gene="rsmA"
                     /locus_tag="ACHJ1G_000969"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4762867.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30898..31275
                     /gene="apaG"
                     /locus_tag="ACHJ1G_000970"
     CDS             30898..31275
                     /gene="apaG"
                     /locus_tag="ACHJ1G_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4762868.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31282..32130
                     /gene="apaH"
                     /locus_tag="ACHJ1G_000971"
     CDS             31282..32130
                     /gene="apaH"
                     /locus_tag="ACHJ1G_000971"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4762869.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32155..32754
                     /locus_tag="ACHJ1G_000972"
     CDS             32155..32754
                     /locus_tag="ACHJ1G_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO4762870.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32790..33257
                     /locus_tag="ACHJ1G_000973"
     CDS             32790..33257
                     /locus_tag="ACHJ1G_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762871.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33356..33535)
                     /locus_tag="ACHJ1G_000974"
     CDS             complement(33356..33535)
                     /locus_tag="ACHJ1G_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4762872.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHJ1G_000975"
     CDS             complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHJ1G_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4762873.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34227..36089)
                     /gene="kefC"
                     /locus_tag="ACHJ1G_000976"
     CDS             complement(34227..36089)
                     /gene="kefC"
                     /locus_tag="ACHJ1G_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4762874.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36082..36612)
                     /gene="kefF"
                     /locus_tag="ACHJ1G_000977"
     CDS             complement(36082..36612)
                     /gene="kefF"
                     /locus_tag="ACHJ1G_000977"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4762875.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36720..38051)
                     /gene="yaaU"
                     /locus_tag="ACHJ1G_000978"
     CDS             complement(36720..38051)
                     /gene="yaaU"
                     /locus_tag="ACHJ1G_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4762876.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38110..38397)
                     /gene="fixX"
                     /locus_tag="ACHJ1G_000979"
     CDS             complement(38110..38397)
                     /gene="fixX"
                     /locus_tag="ACHJ1G_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4762877.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38394..39680)
                     /gene="fixC"
                     /locus_tag="ACHJ1G_000980"
     CDS             complement(38394..39680)
                     /gene="fixC"
                     /locus_tag="ACHJ1G_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4762878.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39731..40672)
                     /gene="fixB"
                     /locus_tag="ACHJ1G_000981"
     CDS             complement(39731..40672)
                     /gene="fixB"
                     /locus_tag="ACHJ1G_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4762879.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40687..41457)
                     /gene="fixA"
                     /locus_tag="ACHJ1G_000982"
     CDS             complement(40687..41457)
                     /gene="fixA"
                     /locus_tag="ACHJ1G_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4762880.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41931..43445
                     /gene="caiT"
                     /locus_tag="ACHJ1G_000983"
     CDS             41931..43445
                     /gene="caiT"
                     /locus_tag="ACHJ1G_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4762881.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43476..44618
                     /gene="caiA"
                     /locus_tag="ACHJ1G_000984"
     CDS             43476..44618
                     /gene="caiA"
                     /locus_tag="ACHJ1G_000984"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4762882.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44747..45964
                     /gene="caiB"
                     /locus_tag="ACHJ1G_000985"
     CDS             44747..45964
                     /gene="caiB"
                     /locus_tag="ACHJ1G_000985"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4762883.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46038..47591
                     /gene="caiC"
                     /locus_tag="ACHJ1G_000986"
     CDS             46038..47591
                     /gene="caiC"
                     /locus_tag="ACHJ1G_000986"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4762884.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47700..48485
                     /gene="caiD"
                     /locus_tag="ACHJ1G_000987"
     CDS             47700..48485
                     /gene="caiD"
                     /locus_tag="ACHJ1G_000987"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4762885.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48491..49081
                     /gene="caiE"
                     /locus_tag="ACHJ1G_000988"
     CDS             48491..49081
                     /gene="caiE"
                     /locus_tag="ACHJ1G_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4762886.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49167..49562)
                     /gene="caiF"
                     /locus_tag="ACHJ1G_000989"
     CDS             complement(49167..49562)
                     /gene="caiF"
                     /locus_tag="ACHJ1G_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4762887.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49823..53044)
                     /gene="carB"
                     /locus_tag="ACHJ1G_000990"
     CDS             complement(49823..53044)
                     /gene="carB"
                     /locus_tag="ACHJ1G_000990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4762888.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53062..54210)
                     /gene="carA"
                     /locus_tag="ACHJ1G_000991"
     CDS             complement(53062..54210)
                     /gene="carA"
                     /locus_tag="ACHJ1G_000991"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4762889.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13184 a        14759 c        14265 g        12387 t
ORIGIN      
        1 aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca
       61 ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg
      121 atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca ccgcagcaca
      181 attagctaat tttacgggtg cagaactcac gctggcggga cgtttttatt gcgtcagggt
      241 tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt
      301 taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg
      361 gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc
      421 cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac
      481 catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca
      541 ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt
      601 tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac
      661 catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggaaaaaga
      721 tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat
      781 tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg aggtgatcga
      841 acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg
      901 cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa
      961 tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt
     1021 cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc
     1081 cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc
     1141 cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc
     1201 gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc
     1261 cattaatcac caggaaatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc
     1321 gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct ccggtatcca
     1381 ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg
     1441 tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg
     1501 catggacgag atggggtata aagaaagtga atataattta gacaatttgt acgacgcctt
     1561 cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt
     1621 catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc
     1681 tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc
     1741 agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac
     1801 tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc
     1861 gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg gcgtcggcct
     1921 ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg
     1981 gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg aaaacaacaa
     2041 ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga cggtattggt
     2101 ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg
     2161 cgcatcacca ccagccatta cgatgtaggc ggtgcagcca ttgataacca cgggcaacca
     2221 ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta
     2281 ggcggtccga aatgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgttg
     2341 cctttgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg
     2401 ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga catcctgtgc
     2461 gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga
     2521 caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga gcgtattgcc
     2581 cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat cgacaaagct
     2641 aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc cacggaatac
     2701 ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat
     2761 ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag
     2821 tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa cgagcaaggt
     2881 tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa aaacatcgcc
     2941 aacccgattg cgcaaattct gtcgctggca ctgctgctgc gctacagcct ggatgccgat
     3001 gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg cattcgcacc
     3061 ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc
     3121 cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg
     3181 ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg
     3241 tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt cgcccggtac
     3301 gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag acgaaagaca
     3361 ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa aactgcaaag
     3421 aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg
     3481 ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg
     3541 ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg
     3601 tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt gaagtccagg
     3661 gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc ggtaaaaccg
     3721 gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc cgtgatttaa
     3781 gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca aaagccggtc
     3841 tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat gcgccgaaag
     3901 gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa
     3961 ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca
     4021 ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccggct tcgtttgccg
     4081 atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggca
     4141 ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca
     4201 ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc
     4261 aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg
     4321 ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg
     4381 cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact
     4441 ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg
     4501 ctgctgcagt gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg
     4561 gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat
     4621 accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg
     4681 catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc
     4741 gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc
     4801 tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt taaagtggtg
     4861 attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg
     4921 gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg
     4981 atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc
     5041 tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag tattgggctt
     5101 accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc gtttatgaat
     5161 taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc
     5221 tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta cccagtagac
     5281 agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca gaattacccc
     5341 ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag gcattaaatc
     5401 ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg
     5461 cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc
     5521 tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc
     5581 cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt
     5641 gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt acatggtgtt
     5701 gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat cctgccagcc
     5761 accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag acggaagcat
     5821 aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccgt
     5881 aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc atgccagaga
     5941 aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcata
     6001 ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac
     6061 accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg cattgccgat
     6121 agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt
     6181 tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat
     6241 ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag
     6301 cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat
     6361 acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa
     6421 aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg
     6481 atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagttc ttcaagcatg
     6541 gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa
     6601 tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac
     6661 ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct
     6721 gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac
     6781 acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg
     6841 ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa
     6901 cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac
     6961 ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg
     7021 cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc
     7081 gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa
     7141 acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa
     7201 ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt
     7261 ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc
     7321 tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat tgtgtcgccg
     7381 acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc gttactgctg
     7441 ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc
     7501 catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca actgaaaatt
     7561 caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa cgtctgggtt
     7621 ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc acagggtgag
     7681 gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc
     7741 acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact
     7801 aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg
     7861 ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc
     7921 ctcatcctga ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag
     7981 cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg
     8041 catgaaggag agatcgagag cgatatctgg cttaacagcg ccaactttac gctgcctctg
     8101 gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg tattccgatc
     8161 gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc
     8221 cagcaactgg tcgccagcaa agcaatggtg ccgcttatcc accactggct gatcattcag
     8281 gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg gctggttcga ttttaaatca
     8341 gcgtggtttg cgccaccgga tccatgattg ctggtagata acaaaatcac tacactaacg
     8401 ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat
     8461 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt
     8521 gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt
     8581 cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact gggggcctgg
     8641 tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac tggtggcgct
     8701 ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata gtaaagccga
     8761 tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg gactatttgc
     8821 caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
     8881 cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc
     8941 gccgcaaagc ctgaaagaat tggttgagag cgatcagaac tggcgggtga tttatcagga
     9001 tctgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag tctatggcga
     9061 tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg
     9121 gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac
     9181 ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag
     9241 cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga
     9301 gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac
     9361 cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg aacaattgac
     9421 caaaccggcc accacgctgg agttcacacc agctgaagtg gctgcacaac gtcaggcatg
     9481 gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta
     9541 agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct gtggtggaac
     9601 gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc
     9661 ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc
     9721 gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca
     9781 atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt
     9841 cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac
     9901 ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta
     9961 ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg
    10021 atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg acaaatcccg
    10081 ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg
    10141 ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct gagttacgac
    10201 tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg ccttgggctg
    10261 gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct gctgcaaggc
    10321 tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aatagtgctg
    10381 gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt aaatcgccag
    10441 ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt
    10501 gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc
    10561 gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg
    10621 atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct caacaacact
    10681 atcggcctgc cacaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc
    10741 ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg
    10801 ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc
    10861 ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt
    10921 ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag
    10981 caaattggct cctatcgcag ccaggacggc gcggtcaccg cgttaattct gctactactc
    11041 tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga
    11101 tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga
    11161 gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgattgc
    11221 cggttttctg acgccagcca gcggttcgct gactatcgac agcgttgatc acaccacgat
    11281 gccgccgtca cgccgcccgg tatcgatgct gtttcaggag aacaacctgt tcagccacct
    11341 gacggtcgcg cagaacatcg ggctggggct taatccggga ttgaagctga acgcggcgca
    11401 gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa tggcgcggtt
    11461 accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg
    11521 cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca
    11581 ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt
    11641 gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg
    11701 gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga gtgcttcggc
    11761 actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc cttatccgac
    11821 ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat caggcaaccc
    11881 gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat acaccggcat
    11941 caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa ttaacggcgt
    12001 cagccacaac aaacggccgc gcgacaaata atgacttaaa cgatcggttg ctttaccgct
    12061 gcgccataac cgccagcaca gccagccacc aacccacaaa aataccgccg tcgccagcag
    12121 caaccattta aactcgccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag
    12181 aatcccaggc aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat
    12241 gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg
    12301 gccaacaaag cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt
    12361 tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa
    12421 acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga tccctgccag
    12481 ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag
    12541 caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac
    12601 caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat
    12661 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
    12721 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
    12781 cttcacaacc ggctcggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
    12841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
    12901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
    12961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
    13021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
    13081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
    13141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
    13201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
    13261 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
    13321 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga taacgaccgt
    13381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
    13441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
    13501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
    13561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
    13621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
    13681 attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta
    13741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
    13801 tctataatca cggcagaaaa gtccacattg gttatttgca cggcgtcaca ctttgctatg
    13861 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
    13921 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc
    13981 ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag
    14041 atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg
    14101 aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa
    14161 accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac
    14221 agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg
    14281 gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa
    14341 gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctac
    14401 attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag
    14461 gacaccgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct
    14521 ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat
    14581 aagtcgctat ggcacgaaag ctggggtggc ttgccgccag ccagtttctt tgatgagctg
    14641 gacccgatcc tcaatcgcca tttaccttcc ccgctgttca ctgacacctg gactgccgat
    14701 attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg
    14761 gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct
    14821 aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag
    14881 agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag cgtggtacct
    14941 ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt
    15001 cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa
    15061 atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg
    15121 tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac accgaacgct
    15181 aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg
    15241 ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc
    15301 gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag
    15361 gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac
    15421 cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac
    15481 atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc
    15541 gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc
    15601 gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt tgcgactcta
    15661 taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca ttggcagcca
    15721 gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa
    15781 tgcgctgaat acggaagcga aactgccctg caaactagtg ttgaaaccgc tgggcaccac
    15841 gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct
    15901 ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc tgaccatgct
    15961 caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt gggacagtat
    16021 cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat
    16081 tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg ataaacaagc
    16141 ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata cccgtcatct
    16201 gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg gtgataaagt
    16261 tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca
    16321 ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg
    16381 ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc
    16441 ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac
    16501 caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg tacagcgtct
    16561 gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg
    16621 catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac
    16681 ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg aagtctgccc
    16741 gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa
    16801 ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg tcgccagcct
    16861 gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacacc
    16921 gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc
    16981 aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct tcagccatgc
    17041 gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat
    17101 tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta
    17161 cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt
    17221 cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcattg
    17281 ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc tctcaggagt
    17341 caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc tgccaaaaca
    17401 caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc gcggcgtctt
    17461 tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata tggtcgtggt
    17521 tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg acacgccaac
    17581 tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata cgcactcgcg
    17641 ccacgccacc atctgggcgc aggcgggtca gtcgattcca gcaaccggca ccacccacgc
    17701 cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg
    17761 cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac agggtatcga
    17821 tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat ggggcaaaaa
    17881 tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata tggggatatt
    17941 ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg ataaacacta
    18001 tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata aaaccacagc
    18061 caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt cagcgtggcg
    18121 caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac agaagtctcc
    18181 ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca agagtccgtg
    18241 gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg tgaacaaggc
    18301 tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta tggcctttac
    18361 tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc
    18421 gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca
    18481 ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg tctgaaaccg
    18541 catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac cacccgccac
    18601 ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta tatgctgggg
    18661 ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc cagccgcccg
    18721 cagttgctgg aaaaactcaa cgcctggttt gccaactacg atcctgatgt gatcatcggt
    18781 tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtctt
    18841 ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg ctttaaaaac
    18901 ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga ggcgctgaaa
    18961 tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga gctattaggc
    19021 gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc
    19081 gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc
    19141 ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa cggcctgccg
    19201 gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc gcgaatgcat
    19261 cgcgctggtt atgtcgcgcc taatctaggc gaagtgccgc cgcacgccag ccctggcggc
    19321 tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga ctataaaagc
    19381 ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt tgaaggcatg
    19441 gcgcagcctg atccagagca cagtaccgaa ggttttctcg atgcctggtt ctcacgggag
    19501 aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt
    19561 cagggtaata aaccactttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg
    19621 cttggcacca ccgcctgccg ctttttcgat ccgcgcctgg catcgtcgat caccatgcgt
    19681 ggtcatcaga tcatgcggca aaccaaagca ttgattgaag cgcagggcta cgacgttatc
    19741 tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga agaagaggcg
    19801 gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga aacgctgcaa
    19861 aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg ccgttttctt
    19921 atgccaacca ttcgcggagc tgataccggc agtaaaaagc gctacgccgg gctgattcag
    19981 gagggcgaca agcagcggat ggtgtttaaa gggctggaaa ccgtgcgcac cgactggacg
    20041 ccgctggccc agcagtttca gcaggagctg tacctgcgta tcttccgcaa cgagccatat
    20101 caggagtacg tccgccaaac catcgacaaa ctgatggcgg gtgaactgga tgcgcggctg
    20161 gtttaccgta aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat
    20221 gtacgcgccg ctcgccttgc cgatgaagaa aatcaaaagc gcggtcgccc cttgcaatat
    20281 cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac
    20341 caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag
    20401 ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca acttgggcta
    20461 ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca
    20521 tccagtacca tagcgccctt tccattcctg gacctgaata acgccactac ctcataagca
    20581 cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta
    20641 cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg
    20701 cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg
    20761 cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc
    20821 atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata
    20881 tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata
    20941 gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg
    21001 accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt
    21061 acagcggcct gcgcggtcag cgtaccagcc tgatcccgca tcagctcaac atcgctcatg
    21121 atgttggtcg ccgccacgcg ccgcgcgttc tgctggctga cgaagtgggt ttagggaaaa
    21181 ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc
    21241 tgattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca
    21301 acctgcgttt tgcgctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca
    21361 acccgttcga caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac
    21421 agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc
    21481 acctggtgtg gagcgaagac gcgccgagcc gcgaatatca ggctattgaa caactggcag
    21541 agcacgtgcc gggcgttctg ctgctgaccg caaccccgga acagttggga atggaaagcc
    21601 acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg
    21661 aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca
    21721 aactgagcaa tgacgaactg aacatgcttg gcgagatgat cggcgagcag gatatcgagc
    21781 cgctgttgca agcagcaaac agcgacagcg aagatgccca gagcgcccgt caggaactgg
    21841 tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac acccgtaacg
    21901 gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa gctgccgcta ccgacgcagt
    21961 atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg
    22021 ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct
    22081 ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc
    22141 agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc
    22201 gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg
    22261 accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag
    22321 aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtaatg tttgacctgc
    22381 cattcaaccc ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc
    22441 acgatattca gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg ctggtgcgct
    22501 ggtatcacga aggtctggat gcattcgagc acacctgccc gaccggacgc actatttacg
    22561 atagcgtcta caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg
    22621 acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacaa ctggaacagg
    22681 ggcgtgatcg cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag
    22741 aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg
    22801 atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg accccgtccg
    22861 atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg
    22921 atcgtgaagt ggcgctggcg cgtgaagatg ctcagtttat tacctgggaa catccgctga
    22981 tccgcaacgg tctggatctg attctttctg gcgataccgg tagcagcacg atttcactgt
    23041 taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttac gtggtcgaag
    23101 cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc
    23161 tgctggataa aaacggcaac aacctggcgg cccaggtgga gtttgaaacc tttaaccgcc
    23221 agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg
    23281 ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg
    23341 atgccgcacg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc
    23401 tgcgcgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc
    23461 agcaggtaat ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg
    23521 ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga
    23581 accctggttg gttatcctgt atcaggatga ccacattatg gtggttaaca agccgagcgg
    23641 tttgttgtct gtgccgggtc gtctggaaga gcacaaagat agcgtgatga cgcgcattca
    23701 gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat
    23761 tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga
    23821 gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag aaggtctggt
    23881 ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac
    23941 gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gtggcggata acacggcaag
    24001 agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct
    24061 gggtcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc
    24121 acgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat
    24181 gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg taggcggata
    24241 aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt
    24301 cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata
    24361 tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc
    24421 tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat
    24481 ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgtcgg
    24541 gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa
    24601 ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa
    24661 ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa
    24721 cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac
    24781 actgcgaaac tggcgcatct tttcgcgcag cgggtaatta tctgattttc ccactcggaa
    24841 cgcgttttgc gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct
    24901 ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac
    24961 ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat
    25021 tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca
    25081 aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat
    25141 atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt
    25201 accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg
    25261 tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag
    25321 agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac
    25381 aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg
    25441 ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc
    25501 caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg
    25561 ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca
    25621 tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag
    25681 gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc
    25741 aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct
    25801 gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac
    25861 aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg
    25921 gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg
    25981 cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt
    26041 tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca
    26101 ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg
    26161 atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat
    26221 tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata
    26281 aagtctatga agatgaaccc ccgaacgatg acagttcacg tcgttggtta ttctactgga
    26341 accactccgg ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg
    26401 atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa
    26461 cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc
    26521 agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg
    26581 ttaattacta ccagaatgat gttggtccat ttgatacgcg tatttacggc caggcagtgc
    26641 actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca
    26701 tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa
    26761 cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg
    26821 aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg
    26881 atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt
    26941 acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg
    27001 actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta
    27061 accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta
    27121 atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa
    27181 catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact tactggcgta
    27241 tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag
    27301 cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt
    27361 accgttacgc cagcccggaa tatattcagg ctacgctgcc taagcactat tccactgctg
    27421 agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc
    27481 gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta
    27541 tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc
    27601 tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca
    27661 tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga
    27721 acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg
    27781 gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg
    27841 atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat
    27901 aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct
    27961 gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt
    28021 ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat
    28081 gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag
    28141 atgcgcagcc gtctggctta cgatggactg aactacaaca cctatcgtaa ccagatccgc
    28201 aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg
    28261 ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag
    28321 ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac
    28381 gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt
    28441 aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg
    28501 ggccgtattc aggagttgcc cgggatcttc gcccaggcat taagcaccgc gaagaaaggc
    28561 gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg
    28621 cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa
    28681 ccgtcgccga tcatgactga cgaacaggcc cgtgtgaaac tggaacagat tgctgctgat
    28741 atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc
    28801 tctgctaacc agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc
    28861 cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc
    28921 ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag
    28981 aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg
    29041 atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc
    29101 aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc
    29161 ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg
    29221 accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg
    29281 cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca
    29341 ctgcggggca gttagcgatt gaaaatggac attacgtggt ggagacgctg gcgcgagcgt
    29401 gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg
    29461 tcattaacga cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc
    29521 aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga
    29581 cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga
    29641 ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg
    29701 tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca
    29761 ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac gggccgctgc
    29821 ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt
    29881 accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta
    29941 cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg
    30001 gacgtggcga agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga
    30061 ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg
    30121 gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca
    30181 aaagggccag gcaatggtcg aaatcggccc cggtctggcg gcattgaccg agccggtcgg
    30241 cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca
    30301 aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa
    30361 ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta
    30421 taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat
    30481 gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc
    30541 gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt
    30601 tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc
    30661 tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga
    30721 agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga
    30781 ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca
    30841 atattgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg
    30901 atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca
    30961 cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg
    31021 ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa
    31081 gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag
    31141 tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg
    31201 atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc
    31261 acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac
    31321 gatgaactgt tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg
    31381 ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg ctatgtgaaa
    31441 tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta
    31501 tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg
    31561 gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag
    31621 aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctaca gaccgccaaa
    31681 gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact cctatccctt ctttctcgac
    31741 gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg
    31801 cgttttatca ccaacgcctt tacccgtatg cgtttttgct ttccaaacgg tcaactggat
    31861 atgtacagca aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt
    31921 cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc
    31981 aaaggtacgc cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta
    32041 acctgcctgc gctgggaaga taagcagtat tttgtccagc cgtcgaaccg gcataaggat
    32101 ttgggtgagg gagaggcggt cgcgtcttaa cgaattacca ttcagggagg aataatggct
    32161 gactattttg aaatacgtgc tgattacaat cagcacacca tcacgattta tcaggcttac
    32221 aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat ttggcgcacc tttttcattc
    32281 aaccgcatga cctggattaa accctctttt atgtggatga tggagcgtag caactgggga
    32341 ctgaaaaaag atcaacaaca cattctcgca atccgaatca aacgcacctt ctttgataca
    32401 ttgctggaac aagcggtgct aaccacgccg gaagcccatg tgtatccaca tgcaggaatt
    32461 tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt gggacccaga acgcagcata
    32521 aatggtaaaa aactggagca ccgatcatta cagcttggta tttctcgtaa cttgatcagt
    32581 caatttaacg aagacgcgat tgttgctatt gatgatctga cgccgctggt gcgaaagtgt
    32641 cataaccttt tgataaacgg caaaacaaca caggcgaaat ccttcctgcc gccagaaaaa
    32701 atttacccag ttagtgcagc agcgagaaag gctttgggaa tgaaaaattc ctgagacaaa
    32761 tacgcaccta ttaagcggaa aatatctaag tgcgacaaat caacaccggg cttaatacca
    32821 gagaaattat tctggaaatg attgacgaag cagatgcccc cccgtttgct gagcagcgga
    32881 cctggcatcg caggcgaatc gcttttctat gtgattgcat cagcgttgct gtcgactacg
    32941 gcttcttctg ttctgtctct cagcagtcaa taatgaacat aaccaagatg gctgaagatg
    33001 tatggataac agaaagtaaa acgacccaac tcattgatat ttggcaacaa cttaaacagc
    33061 tttatccgcc agaaactatt ccctacgccc agaagttacc cactaaggaa ttgcttgaac
    33121 tgctactttc tagtcctgac gatcttcgaa taattgaaat tcctgacatc atcggtgatg
    33181 tttttgatga ccaaacctgg attcgattat tcaaaaaaca ctttgctgat gttattcagc
    33241 tacgatgtga tgtgtaacaa tccaaaatat atcgccagac gatagaccaa aaagaaagta
    33301 tttagcagat actcaacatt ctgacttctg tacagaaata gcagagttta aaaacttaaa
    33361 acttccggta ctcatcagag aaacatccag tttcttctgc aaattcattt aatgccgaga
    33421 tggcatcctc gtttacttca ttccactgta gagtggcctg aaggtttaga cctacgcaat
    33481 gagcgcgact cacaaaatcg cgctcactgg taaaatcaaa cgtcttatcg ttcaatttgt
    33541 agtcctggca aaaaattacc gccgctccag aatctcaaag caatagctgt gagagttctg
    33601 cgcatcagca tcgtggaatt cgctgaatac cgattcccag tcatccggct cgtaatccgg
    33661 gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tctgcgcttt
    33721 tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc
    33781 acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg
    33841 ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgattg attcccaggt
    33901 atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc
    33961 aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc
    34021 aatcagactg atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg
    34081 taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga
    34141 gtccacgctc tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg
    34201 taaagtgtga tttacgtcac tctttattag gatgagggtt tcgtttccgg ttcatccgcc
    34261 atgttgccgg tatgtttacc ttcttcggtt ccctgccagc catgtcgttg aattaatgac
    34321 agatgttcgc ggtcctcggt aatgatctca cttaacatcg cgctggtgcg tttatagacc
    34381 gccgcgcggg ctttggtgtc gttctcaacc attgccatct cttccaccat ctgaatatta
    34441 aagcggcgga acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc
    34501 agtgccagac gcccggtttt cagcgcacct tcgaaggttt cacgctccgg cttttcaacg
    34561 cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa
    34621 tgcgggaaat gttctttcac catctccgtc agttgcaggt tggtttgcgg atcatcaata
    34681 gcattaatca gcacttccgc tttcgccgct ccggcagatt ccagtaaatc catccgcgtt
    34741 gcatcgccat aaaacacttt cataccaaat ttacgcagcg tttcgatatg gtccggatcg
    34801 tgatcgagca ccaccatttt caccccgctg gagagcagta aacgtccggt aatctgccca
    34861 aaacgaccga atccggcgat aatcacgcgc ggctgttctt cgtcgatctc atcggcttca
    34921 cgcgcttcct cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt
    34981 gctgccatcg acagcgccac cgccagggtc agcgatttcg cccactccgg ctccagcaca
    35041 ttcgccatct gcgccgcgcc aaataccaca aaggcaaact cactgccctg ccctaacaac
    35101 accgcaaacc aacgacgctg tttatttggc acttgcaacg gtcgggcaat cagccacagc
    35161 atggcgattt tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa tgggttttca
    35221 agcagcgtgc caaagtctat cgacatgcca acaccgatga aaaacagccc caacagcaaa
    35281 cctttaaatg gttcgatatc gctctccagc gcatgacggt attccgagct tgccagcagt
    35341 acgcccgcca gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca
    35401 aagccaaaca cgaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggct
    35461 acaaaacgca gcgccggacg cgtgacatag cgccccagca ataccaccag caccagcgca
    35521 cccgccactt ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc actgctcgtt
    35581 gccagtagcg gaatcatcgc caccagcggg atcgccgcga tatcctggaa cagcagcacc
    35641 gcaaaggcac tgcgacccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg
    35701 gcaatcgccg tagaggagag cgctagcgtc atgccgatca attccgcgac ctgccagcgc
    35761 agcccaagta acatgcagaa cagccccagc aggccgccgc aaatcaccat ctgcaatgcg
    35821 ccaccgccga acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg
    35881 ataataaaca gcatcagcac caccccaatc tcggcaaagt gcagaataga ttcggcatcg
    35941 gtcaccagtc gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagtacc
    36001 gatcccagac caagacgtac cgcaatgggt acaatcagcg ctgccgaacc gagataaatc
    36061 agcgcctgaa tcagcgtatg gctatccatg atgggcctcc tgccattcca gcagacgttg
    36121 cttatagtga cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat
    36181 ggcaaacggt ggcagccagt tcagtccgca gtagattgcc gtcgcctgta gcggctgcga
    36241 cagcacatca aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt
    36301 caccgcccac agcaaatgtt tgccatgcag cgccgtgccg ccatgaccgt aagcccagcc
    36361 gtgggagaaa actttatcga tccaaagttt gaggagcgga ggaatgctgt accactgcat
    36421 cgggtgctgc cagacgatca gatcggcgcg agacagcgcc tcctgctcgg cggcaatatc
    36481 gatattgaag tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct
    36541 tgcctgttca agcatccgtt tattcgcatg ggaatgatgc ggatacggat gcgcataaat
    36601 tataagaatc ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa gtgtagtcat
    36661 atattcacgc ggcttatagt caataatatt gattcagaaa agagagaaat ctgaacaatt
    36721 taccccattc tctgcccgcg gatcgtcata ttgctggttt gcgccagtga catccctcga
    36781 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    36841 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca ggtcgaaaca
    36901 atggtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatctgtc
    36961 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    37021 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    37081 gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    37141 tttaaccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacgttc
    37201 cccagcgccg cgtttttgcc aacacccaat cccaacaaac caacgatttg cgggccaaag
    37261 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    37321 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca
    37381 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    37441 cctttgcgta ataaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaac
    37501 aaacagggga tcgccgcgct acccagcatc cagcgccagc cgccttccac atcataaagc
    37561 cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatggcggc gataaagctg
    37621 atggaaaacg cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga
    37681 taatctgcac cgatgacaat gccgataagt acccgcatca ccaacagttc gacgggggat
    37741 gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    37801 ttgcgccgtc cgactttatc ggaaatatag ccaaacagcg atgtgccaac gaacagcccg
    37861 gcgagcgttc ccgcgcccaa caagccaatc cagtcagcgt ccagttttag cgccggagtc
    37921 agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa cggaccaccg
    37981 cttccccaca gcaaaatgcg gcggtgaatt gaggagaatt tgagatcgtc aaagtttctg
    38041 gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc gttacgtatc
    38101 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    38161 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    38221 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    38281 cagcagctcc agggcctgtt tatcggcatc ggccttcaca acaatgtgcg gatgctcttc
    38341 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    38401 acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg
    38461 cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc
    38521 gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc aaggaaggcc
    38581 ggtagtttct ggtacatacg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    38641 tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct
    38701 gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    38761 cacattccgg cggcatcacc ggcaatcaat acgccgtcac caaccaactc cggcagcatg
    38821 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc gcccgcgatc
    38881 agcggtgcca ctgccggatg ctgtttgaaa tcttccagca tttgcggcac cgattttttt
    38941 gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt ttcattggta
    39001 taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag acaagccgcg
    39061 ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa ctcgatcagt
    39121 tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc cagcttttcg
    39181 gcaaggatgg agttcactcc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    39241 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    39301 gtaattaact gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc aaatttactg
    39361 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccattgca gtagtccata
    39421 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacg
    39481 ggggcggagt cggcgaaacc aggaataatg tgttccaggc tgtgggcata gagacgcccg
    39541 ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcccct
    39601 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc cagcaccgac gatgatggcg
    39661 tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccaaaa tgcagccctg
    39721 ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc acggcgtcac
    39781 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatcttta ttgatggcga
    39841 aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag atccccaccg
    39901 ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    39961 tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct atcgccttgc
    40021 aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg acgctgacca
    40081 ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg
    40141 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    40201 ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa
    40261 tgcgttcttc gccaatcgcc agaccaccgt aaaccatgtg tttcactgtc gctttaccgt
    40321 cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt
    40381 ttgccgccag taatttgccg cgacgggtgt ttggcagcag caccaggccg tctgcgccgt
    40441 gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga tcgtccggtt
    40501 tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtacg ccgtcggcat
    40561 cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    40621 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    40681 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    40741 gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc gcagcaacct gttgttcaga
    40801 ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    40861 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    40921 agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc
    40981 aacggtgagg gtatctgcag tcagggagat aattttgctg acgccgttaa ctgccggaat
    41041 attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg aagaaccatc
    41101 gccacagagg atcaaatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt
    41161 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccgggcc
    41221 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    41281 ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    41341 cagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac catcagcatt
    41401 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    41461 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    41521 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    41581 atccaacttt caatattgtt aagttcctca ccaatattga aaacatcgcg tagcaaaaag
    41641 aaattttcga tattgcttta tggatcacca atattgaaag tcagatgcta tttttttact
    41701 cacaacacaa cataacaaac tgattaataa tcaaaattaa cgagaaaagc gccctctatt
    41761 cacccgaaac aaaaatgtga taacaatcac agaatgcagc ttattgaata cccattatga
    41821 gttagccatt aacgcgtcca cgaggttaat aataattata ttaaatgtta acaaaaataa
    41881 aacaaacggg aacgcaaaat aaatattcgt tttcacagtg gaattaactc atgaagaatg
    41941 aaaagagaaa aacgggaata gaaccgaagg ttttctttcc gccgttaata atcgtcggca
    42001 tactttgttg gcttacagtc agagatctgg atgcagcgaa tgtcgttatt aatgctgtat
    42061 tcagttacgt caccaatgta tggggatggg catttgaatg gtatatggtg gtgatgcttt
    42121 tcggttggtt ctggctggtg tttggcccgt atgccaaaaa gcgtttaggt aacgaaccac
    42181 ctgaatttag taccgccagt tggatcttta tgatgttcgc ctcctgtacg tctgctgccg
    42241 tactgttctg gggatcgatt gagatctact actacatctc caccccgccg tttggcttag
    42301 aaccgaactc gacaggggcg aaagagttgg ggctggctta cagcttgttc cactggggac
    42361 ctctgccgtg ggccacttac agcttccttt cagtcgcctt cgcttacttc ttctttgtcc
    42421 gcaaaatgga agtgattcgc cccagctcga cactggtgcc gctggtaggt gaaaaacacg
    42481 ccaaagggtt gttcggcact atcgtcgaca acttctatct cgtcgccttg atcttcgcga
    42541 tgggtaccag tctgggcctt gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg
    42601 gcattccgca taccctgcaa ctggacgcta tcatcattac ctgctggatt atcctcaacg
    42661 ccatttgcgt cgcttgcggt ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt
    42721 acctgagctt cctgatgctg ggttgggtgt tcattgtcag cggtgccagc ttcatcatga
    42781 actacttcac cgattcggtg gggatgttgc tgatgtatct gccgcgcatg ttgttctata
    42841 ccgatcccat cgctaaaggc ggcttcccgc agggctggac cgtgttctac tgggcatggt
    42901 gggtgattta tgctatccag atgagtatct tcctcgcccg catctcccgt ggtcgtactg
    42961 tgcgtgaact gtgcttcggc atggtgctgg ggctgacagc gtcaacctgg atcctgtgga
    43021 ctgtactcgg tagtaacact ctgctgttga tagataaaaa catcatcaac attccaaatc
    43081 tgatcgaaca gtacggtgtg gcgcgcgcca tcattgaaac ctgggccgct ctgccactca
    43141 gcaccgccac catgtggggc ttcttcatcc tctgctttat tgccaccgtt acgctggtta
    43201 acgcctgctc ttataccctg gcgatgtcca cttgccgcga agtacgcgat ggtgaagaac
    43261 cacctctgct ggtgcgtatc ggttggtcaa ttctggttgg cattatcggt attgttctgc
    43321 tggcgctcgg cggcctgaaa ccgattcaaa ccgccattat cgccggagga tgcccgctgt
    43381 tcttcgtcaa cattatggtg acgctctcct ttattaaaga cgcgaaacag aactggaaag
    43441 attaattaac ccccaaaata tcaagaggtt gaaagatgga ttttaattta aatgatgagc
    43501 aggaactgtt tgtcgccggt atccgcgaac tgatggccag cgaaaactgg gaggcctatt
    43561 ttgccgagtg cgaccgtgac agcgtctacc cggaacgttt tgtcaaagca ctggcggata
    43621 tgggtatcga cagtctgctg atccctgaag agcacggtgg tctggacgcg gggtttgtta
    43681 ctctcgccgc cgtgtggatg gagctgggac gtctgggggc accaacctat gtgctgtacc
    43741 agttgccggg cgggttcaac accttcctgc gcgaaggcac acaagagcag atcgacaaga
    43801 ttatggcttt ccgcggcacc ggtaagcaga tgtggaactc agcgattacc gaaccgggcg
    43861 cgggctccga cgtgggtagc ctgaaaacga cttatacccg tagaaatggt aagatttatc
    43921 ttaatggtag taagtgtttt attaccagca gcgcctacac cccgtacatc gtggtgatgg
    43981 cgcgcgacgg ggcttctccg gacaaacctg tctacaccga atggtttgtt gatatgagca
    44041 aaccgggcat caaagtgacc aaacttgaaa agctcggtct gcgtatggat agctgctgtg
    44101 aaatcacctt tgacgacgtg gaactggacg agaaagacat gttcggtcgg gaaggtaacg
    44161 gctttaaccg cgtcaaagaa gagttcgacc atgaacgttt cctggtagcc ctcaccaact
    44221 acggtacggc gatgtgcgcc tttgaagatg cggcgcgcta cgccaatcag cgcgtgcagt
    44281 ttggcgaggc tattggtcgt ttccagttga ttcaggaaaa attcgcccac atggcgatca
    44341 aattaaactc catgaaaaac atgctgtatg aagcagcgtg gaaagcagac aacggcacca
    44401 tcacctctgg cgatgcagcg atgtgcaaat acttctgcgc caatgcggca tttgaagttg
    44461 tggatagcgc aatgcaggtg ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc
    44521 gcttctggcg tgatctgcgt gtagaccgcg tctccggggg atctgacgaa atgcagatcc
    44581 tgacgctggg tcgcgcggtg ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga
    44641 cgctaatgcg tcttatcagg cctacgggag cacgaatgta ggccggataa agcgtttacg
    44701 ccgcatccgg cagtcatgcg ctacataaat ttttcaggag ctaataatgg atcatctacc
    44761 catgccgaaa ttcgggccgt tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc
    44821 cgggccgttc gccgggcaaa tgttcgcaga atggggtgcg gaagttatct ggatcgagaa
    44881 cgtcgcctgg gccgacacca ttcgcgttca accgaactac ccgcagctct cccgccgcaa
    44941 tttgcacgcg ctgtcgttaa atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt
    45001 aatggaaacc accgatatct tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg
    45061 cattaccgat gaagtactgt ggcagcacaa cccgaaactg gttatcgctc acctgtccgg
    45121 ttttggtcag tacggcaccg aggagtacac caatcttccg gcctataaca ccatcgccca
    45181 ggccttcagt ggttacctga ttcagaacgg tgatgttgac cagccaatgc ctgccttccc
    45241 gtataccgcc gattactttt ctggcctgac cgccaccacg gcggcgctgg cagcactgca
    45301 taaagtgcgt gaaaccggta aaggcgaaag tatcgacatc gccatgtatg aagtgatgct
    45361 gcgtatgggc cagtacttca tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat
    45421 gagcaaaggt aaagatccct actacgccgg ttgcggtctg tataaatgcg ccgacggcta
    45481 catcgtgatg gaactggtgg gcattaccca aattgaagag tgctttaaag atattggcct
    45541 cgcacatctg ctcggtacgc cggaaatccc ggaaggcact cagcttatcc accgtatcga
    45601 atgcccttac ggcccactgg tagaagagaa actcgatgcc tggctggcgg cacataccat
    45661 cgcagaagtt aaagaacgct ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt
    45721 accggaactg gaaagcaatc cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac
    45781 gatggatggt cgcacctgca aagggccgaa catcatgccg aaattcaaaa ataaccccgg
    45841 acaaatctgg cgcggaatgc cctcacatgg catggacacg gctgccattt tgaaaaatat
    45901 cggctacagc gaaaacgaca ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga
    45961 ctaaacgcgt ggcgtcagtc ggcttgcctg actagcgccc gcccaataac aaacgaacaa
    46021 aaatggatag aggtgcaatg gatatcattg gcggacaaca tctacgtcaa atgtgggacg
    46081 atctggcgga cgtttacggt cataaaacgg cgctgatttg tgaatccagc ggcggagtcg
    46141 ttaaccggta tagttatctt gagttaaatc aggagattaa ccgcacggca aacctgtttt
    46201 atacgctggg gattcgcaaa ggcgacaagg ttgcactaca tctcgacaac tgcccggaat
    46261 ttatcttttg ctggttcggg ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc
    46321 gcctgttacg cgaggaaagc gcgtggatcc tgcaaaatag ccaggcgtgc ctgctggtga
    46381 ccagtgcgca attctatcct atgtatcaac agattcagca ggaagatgcc actcaattgc
    46441 ggcacatttg cctgacagat gtggcacttc ccgctgatga tggcgtgagt tcgtttactc
    46501 aactgaaaaa tcaacaacct gccaccttgt gctatgcacc gccgctattg actgacgata
    46561 cggcggaaat tctcttcacc tccggcacca cctcccgacc gaaaggtgtg gtgattaccc
    46621 attacaacct gcgcttcgct ggatattact ccgcctggca gtgtgcactg cgtgacgatg
    46681 acgtctacct gacggtaatg cctgcgtttc atatcgattg ccagtgtact gcggcgatgg
    46741 cggcgttttc tgccggggcc acctttgtgc tggtcgagaa atacagcgcc cgcgccttct
    46801 ggggacaggt gcagaagtac cgcgccacca ttaccgaatg tattccgatg atgatccgta
    46861 cgttgatggt gcagccgcct tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt
    46921 tttatctcaa cttgtcggag caggaaaaag atgcgttttg tgaacgcttc ggcgttcgct
    46981 tgctgacgtc ttatgggatg acggaaacca ttgtgggcat tatcggcgat cgccctggcg
    47041 ataaacgacg ctggccgtcg attggtcggg cggggttttg ctacgaagcg gagatccgcg
    47101 acgatcacaa tcgcccgctc ccggcaggtg agatcggtga aatctgtatt aaaggcgtac
    47161 cagggaaaac catcttcaaa gagtattttc tcaacccgaa agccactgcg aaagtgctgg
    47221 aagccgacgg ctggctacat accggcgata ccggatactg cgacgaagag ggcttttttt
    47281 atttcgtcga tcgccgctgc aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg
    47341 agctggaaaa tatcatcgcc acccatccaa aaattcagga tatcgtggtt gtgggtatta
    47401 aagattcgat tcgcgatgaa gccatcaaag catttgtggt gctgaatgaa ggtgaaacat
    47461 tgagcgaaga ggaatttttc cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct
    47521 cttatctgga gatcagaaaa gatctgccac gtaattgctc ggggaaaata attagaaaga
    47581 atctgaaata aatgaaacag cagggaacta ccctgccagc taataatcac gctataaaac
    47641 aatatgcatt ttttgcaggg atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa
    47701 tgagtgaatc attacatctg acccgcaatg gatcaattct ggaaattacc cttgatcgtc
    47761 caaaagcgaa tgctattgat gcaaaaacca gctttgaaat gggcgaagta tttctaaatt
    47821 tccgtgacga tccgcaatta cgtgtcgcca ttattaccgg tgccggagag aagttctttt
    47881 ccgcgggctg ggatttaaaa gcggcagcag aaggcgaagc accggatgct gactttggcc
    47941 cgggtggatt tgcgggatta accgaaattt tcaatctcga caaaccggtt atcgcagctg
    48001 tgaacggcta tgcctttggc ggcggtttcg aactggcgct ggcggcagat tttattgttt
    48061 gtgccgataa cgccagcttc gccctgccgg aagccaagct gggcatcgtt cctgacagcg
    48121 gcggcgtgct gcgtctaccg aagatcctgc cgcctgccat cgtcaatgaa atggtgatga
    48181 ccggcagacg aatgggcgca gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta
    48241 gccaggcgga actgatggat aacgcccgcg aactggcgca gcagctggtt aacagcgccc
    48301 cgctggcgat tgcggcgctg aaagagatct tccgcaccac cagcgaaatg ccggtagaag
    48361 aagcgtatcg ctatattcgc agcggcgtgt tgaaacacta tccgtcggtt ctgcattcgg
    48421 aggatgccat tgaagggccg ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac
    48481 gttaacaacc gtgagctatt acgcctttga ggggttaatt ccggtggttc acccgacggc
    48541 gtttgtccat cccagtgccg tcttgattgg cgatgtgatt gtgggagccg gtgtctacat
    48601 cggcccactc gcctcactgc gtggtgacta cgggcggttg atcgtgcaag cgggagccaa
    48661 tattcaggat ggctgcatta tgcatggcta ctgcgacact gacaccatcg ttggggaaaa
    48721 cggccatatc gggcacggag cgatcctgca tggttgtgtg attggtcgcg atgcattggt
    48781 cgggatgaac agcgtgatta tggatggcgc ggtcattggc gaagagagca ttgttgccgc
    48841 catgagcttt gtcaaagcag gctttcacgg cgagaaacgc cagttgttga tgggtacgcc
    48901 agcccgcgct gtacgcagtg ttagtgacga cgagttacac tggaaacggt tgaataccaa
    48961 agagtatcag gatcttgtcg ggcgctgtca tgcatcgtta catgaaacac agccactgag
    49021 gcaaatggag gaaaatcgcc cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg
    49081 atcttgccgg atgcgacctg gttgctggat aagatgctac aacatcgcat ctggcaagca
    49141 ggacgcccgg tatcaacgag ataaaattaa cgacgcatac tctttgacaa catcatctgc
    49201 cacttctgct cgcggttaag ttccgtgagc ggaacggcag gaatacgcgg cgtctttctt
    49261 acgccgacta atttatcccg gctgttattg cgcaggcgcg cgtaaatttg ctcatcgata
    49321 tcgaccactt ttaccagtcg ctggcactgg catccccgtc cttccagctt attagggatc
    49381 atcttaactt cgcagcttat ttctgcgact tccgacagaa tataagtcag ggtattaacc
    49441 gccttgctgt gttcaatatc gaaatgaata gagatctctc ttgctatcac ccaccgattt
    49501 tcagccatca tccattcggc gattaacaag tagagtggtt tttcaacata tccttcacac
    49561 atacgaactc cagtttcaca aattaaaagt ctggctatga ctcgttttga agcaattaaa
    49621 caaggccata ttaaaattgt ggatatatcg atagtatgca acttcaccgt cgataagtgt
    49681 gagctaaatc ctgcttatgt catcaaaaat atcgattccc caaaagattt acttaatggg
    49741 caaaaaaaac ggtcctcatc agagaaccgt attacttatt cgatcaaatt agcggatgaa
    49801 aaatatccgc catgacacgc tattatttga tctgcgcgtg catttcctgc accgaaatta
    49861 ctttttcggt cgcgtcggca ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg
    49921 tgtcataatg cactttatat tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg
    49981 cgcgacggcc tgaagtggtg ttgatgatgt aggtatattc gccattcttg atgcggtcct
    50041 gaatgtgcgg acggccttca tgcaccttgt tcaccagacg cggattaatg cccgcttcgc
    50101 ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc agcagttttg
    50161 ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac ggaaagcaac gcacgaccgt
    50221 gtttcttcat ggtggagttg ctgcccagct gcgctttggc aaacgcttca gcgaaggtgc
    50281 ggcccacgcc catcacttcc ccggtggagc gcatttctgg ccctaacagc gggtcaacac
    50341 ccgggaattt attgaacggc agcaccactt ctttcaccga gtagtacggc gggataactt
    50401 ctttggttac gccctgctca gccagcgatt tgccagccat cacacgcgcc gccacttttg
    50461 ccagcggtac gccggtggct ttggagacga acggaacggt acgcgccgca cgcgggttaa
    50521 cttcaatcag gtagacttcg ttgtttttca ccgcaaactg cacgttcatc agaccgcgca
    50581 cctgcaattc gaaggccagt ttctgcacct gctggcgcat cacatcctga atttcctgac
    50641 ttaaggtgta ggctggcaga gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga
    50701 tgtgctccat gatgccgcca atcagcacca tttcgccgtc gcagatggca tccacgtcaa
    50761 cttctaccgc gtcatcaagg aagtgatcca gcaacactgg cgcatcgtta gacacgctga
    50821 ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata gacgatttcc atcgcccgac
    50881 cgccgagaac gtaagacgga cgtaccacca gcgggtagcc aatctctttc gccttctcta
    50941 ccgccatttc aatagcggta acggtggcgt tcgccggttg tttcagtttc agacgctcaa
    51001 ccgcatgctg gaagcgttca cggtcttctg cacggtcgat agcatccggg ctggtgccga
    51061 taaccggtac gccagcagct tccagcgcgc gcgccagttt cagcggggtc tgaccgccgt
    51121 actgaacgat aacgcctttc ggcttctcaa tacgcacgat ttctagcaca tcttccagtg
    51181 ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc ggtggagacg gtttccgggt
    51241 tacagttaac cataatggtt tcgtaaccgt cttcgcgcag tgccagcgag gcgtgtacac
    51301 agcagtagtc gaattcgata ccctgaccga tacggttcgg gccgccgccg agaaccatga
    51361 ttttttcacg gtcggtagac ggattcgctt cgcactcttc ttcataagtg gagtacatgt
    51421 aagcggtgtc ggtggcgaac tctgccgcac aggtatccac gcgcttataa accgggtgca
    51481 ggtcatactg gtcgcgcagc ttgcggattt ccgcttcgcg cacgcccgcc agttttgcca
    51541 gacgcgcatc ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg ttcaggccag
    51601 tgatgcccac ttccgctact ttctcttcca gacgcaccag ttcttcaatc tgtaccagga
    51661 accagcggtc aatattggtc aggttgaaca cgccgtccac agacaggccc gcacggaagg
    51721 catcggcgat gtaccagata cgctctgcgc cagcatcttt cagttcgcga cggattttgg
    51781 ttaacgcttc cgggtcatcg aggctcactt tcgggtcgaa tccagtcgca ccgacttcca
    51841 ggccgcgcag cgctttttgc agggattcct gctgcgtgcg accaatcgcc atcacttcgc
    51901 caaccgattt catctgagtg gtcagacggt cgttagcacc agcgaatttt tcgaagttga
    51961 agcgaggaat tttagtaacc acatagtcga tggacggctc gaaggaggcc ggagtacgtc
    52021 cgccagtgat gtcgttcatc agttcgtcga gggtgtaacc caccgccagt ttcgccgcca
    52081 ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg gacacgcgcg
    52141 ggttcatttc gataacaatc aggcgaccgt ttttcgggtt caccgcaaac tggacattgg
    52201 aaccaccggt ttcaacgccg atttcacgca gcaccgccat cgaggcgtta cgcatgattt
    52261 gatattcttt gtcggtcagc gtttgggctg gcgcgacagt gatggagtca ccggtatgga
    52321 tgcccatcgc atcaaagttt tcgatagagc agacgatgat gcagttgtcg tttttatcac
    52381 gcaccacttc catctcgtac tctttccagc caatcagcga ctcatcaatc agcaactctt
    52441 tggtcggaga gagatccaga ccgcgggcgc aaatttcttc aaactcttcg cggttataag
    52501 cgataccgcc gccgctaccg cccatggtaa aggatgggcg aataatgcac gggaagccca
    52561 cgtcagcggc aaccgccagc gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt
    52621 ccagaccaat tttcttcatc gctacgtcga aacgacggcg gtcttctgct ttatcaatcg
    52681 catcggcggt cgcaccaatc atagtcacgc cgaactcttc caacacgccc tgacgctcca
    52741 gctccagcgc gcagttcagc gccgtctgac cgcccatcgt tggcagcacc gcgtccgggc
    52801 gctctttttc aataatcttg cgtaccactt cccagtgaat cggctcgatg taggtcgcat
    52861 cggccatttc cgggtcggtc atgatggtcg ccgggttgga gttcaccaga atgacccggt
    52921 aaccctcttc gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct
    52981 gaccgataac aatcgggccc gcacccagaa tcaggatact ttttatatct gtacgttttg
    53041 gcatggctct tttactcctg attacttagc ggttttacgg tactgctcaa ttaactcgat
    53101 aaagtggtcg aacaacggcg cggcgtcgtg tggaccaggg ctggcttcag ggtgcccctg
    53161 gaagctgaac gccggtttat cggtgcgatg aatgccctgt aacgtaccgt cgaacaggga
    53221 tttatgcgtg acacgcaggt ttgctggtaa tgttgcttcg tccaccgcaa aaccgtggtt
    53281 ctgggcggtg atcatcacca cgtttttctc aacatcttta accggatggt tgccgccgtg
    53341 gtgaccaaat ttcattttga cagtcttcgc accgctcgcc agcgccagca gctgatgacc
    53401 gaggcagatg ccgaagaccg gaatatcggt ttcgaggaat ttctggatgg cggtaatggc
    53461 gtaatcgcac ggggccgggt cgccaggacc gttggagagg aagataccgt ctggattcat
    53521 tttcagcaca tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac agcctctgtc
    53581 caccaacatg cgcaggatgt tgcgcttggc accaaaatca taagccacga cgtggaacgg
    53641 cagctcgtct tcttttttcg cttctggcag gccgccggtc aacgtccagc tcccttgtgt
    53701 ccagctatag gcttctgcgg tggtcacttc ttttgccaga tccatgccat tcagacctgg
    53761 gaacgcgcgg gctttttcta acgccaacgc cgcatccggg ttatcgcctg cgataatgca
    53821 gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc
    53881 aatcgccacg atgttatggc gcttcaggta agaagagagg tcttcggtat tacggaagtt
    53941 gctggcaatc agcggcaggt cgcgaatcac cagaccttgt gcatgtacct gagaagattc
    54001 ttcatcggcg tcattagtgc cgacattgcc aatatgggga taagtaagag taacgatttg
    54061 acgagaatag gaaggatcag tgaggatttc ttgataaccg gtcattgaag tattgaaaac
    54121 gacttcccca accgccgaac ctgttgcccc tatggcccga ccgtgaaact gggttccgtc
    54181 ttccagaacc aatagcgctg acttaatcaa aacaccctcc agagaatatt cactcacttt
    54241 atttgcatat taattcacaa tgatgacgta aatcaatgca aatctgctta ccgacgaatt
    54301 tctggcaaac ggcggcattc tggagatatg ggcgctaaaa gtcaacttaa tggcgggatt
    54361 ttttatatta ttttgtggct catgcatgtt ttacaggcat taagcggcaa aaagatcata
    54421 taacctgttc tggaaaacgc ttgcgcagca agaagattgc atgaaataaa agcgagtagg
    54481 gaaaagtgga ccaaatggtc aaaatttaca ttaaaacaac aaaaaccgaa gacaaaacat
    54541 gtaatttttg gcacttatag acaatttaaa aacataacac taaaaataaa agggc
//
LOCUS       DAZFDP010000015        51871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFDP010000015 DAZFDP010000000
VERSION     DAZFDP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHJ1G_000992"
     CDS             <1..261
                     /locus_tag="ACHJ1G_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO4762890.1"
                     /translation="RQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQ
                     EEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            280..978
                     /gene="yjfJ"
                     /locus_tag="ACHJ1G_000993"
     CDS             280..978
                     /gene="yjfJ"
                     /locus_tag="ACHJ1G_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4762891.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1029..1688
                     /gene="yjfK"
                     /locus_tag="ACHJ1G_000994"
     CDS             1029..1688
                     /gene="yjfK"
                     /locus_tag="ACHJ1G_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4762892.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1706..2104
                     /gene="yjfL"
                     /locus_tag="ACHJ1G_000995"
     CDS             1706..2104
                     /gene="yjfL"
                     /locus_tag="ACHJ1G_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4762893.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2114..2752
                     /gene="yjfM"
                     /locus_tag="ACHJ1G_000996"
     CDS             2114..2752
                     /gene="yjfM"
                     /locus_tag="ACHJ1G_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4762894.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2755..3918
                     /gene="yjfC"
                     /locus_tag="ACHJ1G_000997"
     CDS             2755..3918
                     /gene="yjfC"
                     /locus_tag="ACHJ1G_000997"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4762895.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4002..5627
                     /gene="aidB"
                     /locus_tag="ACHJ1G_000998"
     CDS             4002..5627
                     /gene="aidB"
                     /locus_tag="ACHJ1G_000998"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4762896.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5744..6019)
                     /gene="yjfN"
                     /locus_tag="ACHJ1G_000999"
     CDS             complement(5744..6019)
                     /gene="yjfN"
                     /locus_tag="ACHJ1G_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4762897.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6168..6497)
                     /gene="bsmA"
                     /locus_tag="ACHJ1G_001000"
     CDS             complement(6168..6497)
                     /gene="bsmA"
                     /locus_tag="ACHJ1G_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4762898.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6679..7428
                     /gene="yjfP"
                     /locus_tag="ACHJ1G_001001"
     CDS             6679..7428
                     /gene="yjfP"
                     /locus_tag="ACHJ1G_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4762899.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7425..8180)
                     /gene="ulaR"
                     /locus_tag="ACHJ1G_001002"
     CDS             complement(7425..8180)
                     /gene="ulaR"
                     /locus_tag="ACHJ1G_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4762900.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8288..9352)
                     /gene="ulaG"
                     /locus_tag="ACHJ1G_001003"
     CDS             complement(8288..9352)
                     /gene="ulaG"
                     /locus_tag="ACHJ1G_001003"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO4762901.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9707..11104
                     /gene="ulaA"
                     /locus_tag="ACHJ1G_001004"
     CDS             9707..11104
                     /gene="ulaA"
                     /locus_tag="ACHJ1G_001004"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4762902.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11120..11425
                     /gene="ulaB"
                     /locus_tag="ACHJ1G_001005"
     CDS             11120..11425
                     /gene="ulaB"
                     /locus_tag="ACHJ1G_001005"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4762903.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11435..11899
                     /gene="ulaC"
                     /locus_tag="ACHJ1G_001006"
     CDS             11435..11899
                     /gene="ulaC"
                     /locus_tag="ACHJ1G_001006"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4762904.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11913..12563
                     /gene="ulaD"
                     /locus_tag="ACHJ1G_001007"
     CDS             11913..12563
                     /gene="ulaD"
                     /locus_tag="ACHJ1G_001007"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4762905.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12573..13427
                     /gene="ulaE"
                     /locus_tag="ACHJ1G_001008"
     CDS             12573..13427
                     /gene="ulaE"
                     /locus_tag="ACHJ1G_001008"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4762906.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13427..14113
                     /gene="ulaF"
                     /locus_tag="ACHJ1G_001009"
     CDS             13427..14113
                     /gene="ulaF"
                     /locus_tag="ACHJ1G_001009"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4762907.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14242..14517)
                     /gene="yjfY"
                     /locus_tag="ACHJ1G_001010"
     CDS             complement(14242..14517)
                     /gene="yjfY"
                     /locus_tag="ACHJ1G_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4762908.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14844..15239
                     /gene="rpsF"
                     /locus_tag="ACHJ1G_001011"
     CDS             14844..15239
                     /gene="rpsF"
                     /locus_tag="ACHJ1G_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4762909.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15246..15560
                     /gene="priB"
                     /locus_tag="ACHJ1G_001012"
     CDS             15246..15560
                     /gene="priB"
                     /locus_tag="ACHJ1G_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4762910.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15565..15792
                     /gene="rpsR"
                     /locus_tag="ACHJ1G_001013"
     CDS             15565..15792
                     /gene="rpsR"
                     /locus_tag="ACHJ1G_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4762911.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15834..16283
                     /gene="rplI"
                     /locus_tag="ACHJ1G_001014"
     CDS             15834..16283
                     /gene="rplI"
                     /locus_tag="ACHJ1G_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4762912.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16354..17148)
                     /gene="yjfZ"
                     /locus_tag="ACHJ1G_001015"
     CDS             complement(16354..17148)
                     /gene="yjfZ"
                     /locus_tag="ACHJ1G_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4762913.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            17420..17713
                     /locus_tag="ACHJ1G_001016"
                     /pseudo
     CDS             17420..17713
                     /locus_tag="ACHJ1G_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(17777..18202)
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_001017"
                     /pseudo
     CDS             complement(17777..18202)
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            18210..19418
                     /locus_tag="ACHJ1G_001018"
     CDS             18210..19418
                     /locus_tag="ACHJ1G_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4762914.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            19462..19569
                     /locus_tag="ACHJ1G_001019"
                     /pseudo
     CDS             19462..19569
                     /locus_tag="ACHJ1G_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(19553..20191)
                     /gene="ytfB"
                     /locus_tag="ACHJ1G_001020"
     CDS             complement(19553..20191)
                     /gene="ytfB"
                     /locus_tag="ACHJ1G_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4762915.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            20410..21030
                     /gene="fklB"
                     /locus_tag="ACHJ1G_001021"
     CDS             20410..21030
                     /gene="fklB"
                     /locus_tag="ACHJ1G_001021"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4762916.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            21339..22742
                     /gene="cycA"
                     /locus_tag="ACHJ1G_001022"
                     /gene_synonym="dagA"
     CDS             21339..22742
                     /gene="cycA"
                     /locus_tag="ACHJ1G_001022"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4762917.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(22784..22882)
                     /locus_tag="ACHJ1G_001023"
                     /pseudo
     CDS             complement(22784..22882)
                     /locus_tag="ACHJ1G_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23009..23437
                     /locus_tag="ACHJ1G_001024"
                     /pseudo
     CDS             23009..23437
                     /locus_tag="ACHJ1G_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23542..24609
                     /locus_tag="ACHJ1G_001025"
     CDS             23542..24609
                     /locus_tag="ACHJ1G_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4762918.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(24856..25518)
                     /gene="ytfE"
                     /locus_tag="ACHJ1G_001026"
     CDS             complement(24856..25518)
                     /gene="ytfE"
                     /locus_tag="ACHJ1G_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4762919.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(25626..26591)
                     /gene="ytfF"
                     /locus_tag="ACHJ1G_001027"
     CDS             complement(25626..26591)
                     /gene="ytfF"
                     /locus_tag="ACHJ1G_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4762920.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(26699..27559)
                     /gene="qorB"
                     /locus_tag="ACHJ1G_001028"
     CDS             complement(26699..27559)
                     /gene="qorB"
                     /locus_tag="ACHJ1G_001028"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4762921.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            27648..28028
                     /gene="ytfH"
                     /locus_tag="ACHJ1G_001029"
     CDS             27648..28028
                     /gene="ytfH"
                     /locus_tag="ACHJ1G_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4762922.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(28157..30100)
                     /gene="cpdB"
                     /locus_tag="ACHJ1G_001030"
     CDS             complement(28157..30100)
                     /gene="cpdB"
                     /locus_tag="ACHJ1G_001030"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4762923.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            30290..31030
                     /gene="cysQ"
                     /locus_tag="ACHJ1G_001031"
     CDS             30290..31030
                     /gene="cysQ"
                     /locus_tag="ACHJ1G_001031"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4762924.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(31020..31577)
                     /gene="ytfJ"
                     /locus_tag="ACHJ1G_001032"
     CDS             complement(31020..31577)
                     /gene="ytfJ"
                     /locus_tag="ACHJ1G_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4762925.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            31902..32108
                     /gene="ytfK"
                     /locus_tag="ACHJ1G_001033"
     CDS             31902..32108
                     /gene="ytfK"
                     /locus_tag="ACHJ1G_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4762926.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(32170..33513)
                     /gene="paeA"
                     /locus_tag="ACHJ1G_001034"
     CDS             complement(32170..33513)
                     /gene="paeA"
                     /locus_tag="ACHJ1G_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4762927.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(33836..34474)
                     /gene="msrA"
                     /locus_tag="ACHJ1G_001035"
     CDS             complement(33836..34474)
                     /gene="msrA"
                     /locus_tag="ACHJ1G_001035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4762928.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34680..36413
                     /gene="tamA"
                     /locus_tag="ACHJ1G_001036"
     CDS             34680..36413
                     /gene="tamA"
                     /locus_tag="ACHJ1G_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4762929.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            36410..40189
                     /gene="tamB"
                     /locus_tag="ACHJ1G_001037"
     CDS             36410..40189
                     /gene="tamB"
                     /locus_tag="ACHJ1G_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4762930.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            40192..40533
                     /gene="ytfP"
                     /locus_tag="ACHJ1G_001038"
     CDS             40192..40533
                     /gene="ytfP"
                     /locus_tag="ACHJ1G_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4762931.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            40745..40996
                     /gene="chpS"
                     /locus_tag="ACHJ1G_001039"
     CDS             40745..40996
                     /gene="chpS"
                     /locus_tag="ACHJ1G_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO4762932.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            40990..41340
                     /gene="chpB"
                     /locus_tag="ACHJ1G_001040"
     CDS             40990..41340
                     /gene="chpB"
                     /locus_tag="ACHJ1G_001040"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO4762933.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(41420..41950)
                     /gene="ppa"
                     /locus_tag="ACHJ1G_001041"
     CDS             complement(41420..41950)
                     /gene="ppa"
                     /locus_tag="ACHJ1G_001041"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4762934.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            42260..43216
                     /gene="ytfQ"
                     /locus_tag="ACHJ1G_001042"
     CDS             42260..43216
                     /gene="ytfQ"
                     /locus_tag="ACHJ1G_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4762935.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            43356..44858
                     /gene="ytfR"
                     /locus_tag="ACHJ1G_001043"
     CDS             43356..44858
                     /gene="ytfR"
                     /locus_tag="ACHJ1G_001043"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4762936.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            44872..45894
                     /gene="ytfT"
                     /locus_tag="ACHJ1G_001044"
     CDS             44872..45894
                     /gene="ytfT"
                     /locus_tag="ACHJ1G_001044"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4762937.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            45881..46876
                     /gene="yjfF"
                     /locus_tag="ACHJ1G_001045"
     CDS             45881..46876
                     /gene="yjfF"
                     /locus_tag="ACHJ1G_001045"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4762938.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(46909..47907)
                     /gene="fbp"
                     /locus_tag="ACHJ1G_001046"
     CDS             complement(46909..47907)
                     /gene="fbp"
                     /locus_tag="ACHJ1G_001046"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4762939.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            48083..49456
                     /gene="mpl"
                     /locus_tag="ACHJ1G_001047"
     CDS             48083..49456
                     /gene="mpl"
                     /locus_tag="ACHJ1G_001047"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4762940.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(49612..50163)
                     /gene="yjgA"
                     /locus_tag="ACHJ1G_001048"
     CDS             complement(49612..50163)
                     /gene="yjgA"
                     /locus_tag="ACHJ1G_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4762941.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            50257..51609
                     /gene="pmbA"
                     /locus_tag="ACHJ1G_001049"
     CDS             50257..51609
                     /gene="pmbA"
                     /locus_tag="ACHJ1G_001049"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4762942.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            51792..>51871
                     /locus_tag="ACHJ1G_001050"
     CDS             51792..>51871
                     /locus_tag="ACHJ1G_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4762943.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT        12422 a        12700 c        13998 g        12751 t
ORIGIN      
        1 cgccaaatga ttatcgaaac ctttatttgt ccggtaagta gtatcagcaa tccagatgaa
       61 tttaatacct tcttattaag aaatcagaag atgatgccgt tgtcatcggt aggaatctcc
      121 agcgtgcaac aggaagagta ttacattgtt ttcggggcgt tatcgcttaa atcttctctt
      181 gaagatatcc tgcttgagat aacctcgctg gtagataacg cattggatct ggctgaaatt
      241 acagaagaat attcacacta attacaagga cgggtattta tgggaatttt aaaaagttta
      301 tttacgctgg ggaaatcgtt tatctcccag gcggaagaat ccattgaaga aacccagggc
      361 gtgcgcatgc tggaacagca tattcgtgac gctaaagctg aactcgataa agccggaaaa
      421 tctcgcgttg atctgctggc gcgggtgaaa ttaagtcacg ataagctgaa agatttacgt
      481 gagcgcaaag ccagtctgga agcccgtgcg ctggaagcgt taagcaagaa cgttaatccg
      541 tcgttgatta acgaagttgc tgaagaaatc gcgcgccttg agaatctcat taccgctgaa
      601 gagcaagtgt tgtcgaatct ggaagtctcc cgtgatggcg tggaaaaagc agttacagcg
      661 acagcgcagc gtatcgctca gtttgagcag caaatggaag tcgttaaagc cactgaagcc
      721 atgcagcgtg cacaacaggc tgtaacaacc tctaccgttg gcgcatcttc cagcgtttcg
      781 acagcggcag aatccttaaa acgcctgcaa acgcgtcagg ccgaacgtca ggctcgcctg
      841 gatgctgctg cacagttgga aaaagtcgca gacggtcgcg atcttgatga aaagctggcg
      901 gaagccggaa ttggcggtag caataaaagt agcgcccagg atgtattagc aagactgcaa
      961 cgccaacagg gcgagtaatt tttttgccag cctcgctaaa aggctggcaa ctattttaag
     1021 gataaaatat gtctggtttt ttccagcgtc tgtttggcaa ggataataag cctgctatcg
     1081 ctcgcggtcc gctgggactt catctcaata gtggctttac gctcgacacg ttagcgtttc
     1141 gtttgctgga agatgaattg ctgattgcgc tgccgggtga ggaatttacg gtagccgctg
     1201 tcagccacat cgatctgggc ggcggtagtc agattttccg ctactacact tcaggcgatg
     1261 aatttctgca aatcaatacc accggcggcg aagatattga tgacattgat gatatcaagc
     1321 tctttgtcta tgaagagagc tacggtatca gtaaagagag tcactggcgc gaggcgatca
     1381 acgccaaagt gatgggggca atgatcttaa actggcagga aaaacgctgg cagcgatttt
     1441 ttaacagcga agaaccggga aatatcgaac ccgtctacat gctggaaaaa gtagaaaatc
     1501 aaaaccatgc caaatgggag gtccataatt ttaccatggg ctaccagcgg caagtaaccg
     1561 aagatactta cgaatatttg ctgttaaatg gtgaggaatc ttttaacgat ctcggcgagc
     1621 cagagtggtt attttcgcgc gcgttgggtg tcgatatccc gctgacatca cttcatatta
     1681 ttggttaatt acaaggacgt ttacaatgca tatactggat tcacttcttg cctttagcgc
     1741 ctattttttt attggcgtgg ctatggtgat tatttttctt tttatctatt ctaaaattac
     1801 accgcacaac gaatggcagt taatcaaaaa caataatacc gcggcttcac tggcgttcag
     1861 tggtacattg ttaggttacg ttatcccatt atccagtgcg gcaatcaatg cggtgagtat
     1921 tccagactat ttcgcctggg gcgggattgc actggtgatt cagttactcg tttttgctgg
     1981 tgtcagactt tatatgcccg cattaagcga aaaaattatt aatcacaata ccgcagcagg
     2041 aatgtttatg ggaaccgccg cgctggctgg cggtattttt aacgcagctt gtatgacatg
     2101 gtaatggacg atcatggcca gaaaacgcaa atcaagaaat aacagtaaaa ttggtcacgg
     2161 agcgatcagt cgcattggta gaccgaataa tccttttgaa ccgcgtcgca atcgctacgc
     2221 acaaaaatac ttaacgttgg ctctaatggg cggtgccgct ttttttgtat tgaaaggttg
     2281 tggcgatagt ggcgatgtcg ataacgacgg cgacggaacg ttttattcga cagtgcagga
     2341 ttgtattgat gacggtaata atgccgacat ttgcgcgcgt ggctggaaca acgccaaagc
     2401 ggcattttat gccgatgttc cgaagaatat gactcagcag aattgtcagt ctaagtacga
     2461 aaattgctac tacgacaatg ttgaacagag ttggatcccg gtcgtttctg gatttttgtt
     2521 aagccgggtt attcgtaaag atcgcgatga gccgttcgtc tataacagcg gcggatcctc
     2581 atttgcttcc cgccccgtct ggcgcaacac ttccggtgat tactcctggc gctccggttc
     2641 tggcaaaaaa gagtcttact cttcaggcgg ctttaccacc aaaaaagcgt caaccgtttc
     2701 gcgcggcggc tatggtcgtt cttccagcgc ccgtgggcat tggggaggct aatcatgctg
     2761 agacacaacg ttcctgtgcg acgggatctg gaccagatcg ccgctaacaa cggtttcgac
     2821 tttcatatca tcgacaatga aatctattgg gacgagagtc gggcttaccg ttttactctg
     2881 cgccagattg aagagcagat cgaaaaaccg actgcggaac tgcatcagat gtgccttgag
     2941 gtggttgatc gcgcggttaa agacgaagag atcctgacgc aactggcgat cccgccgttg
     3001 tactgggatg tgatcgccga aagctggcgc gcacgcgatc cttcgctgta tggccggatg
     3061 gattttgcct ggtgtggcaa tgcgccggtg aagctgctgg agtacaacgc cgatacgccg
     3121 acctcgttgt atgagtcggc ttatttccag tggttgtggc tggaggatgc ccggcgcagc
     3181 ggcattattc cgcgtgatgc cgatcagtac aatgccattc aggaacgcct gatttcacgc
     3241 tttagcgagc tttatagccg ggagccgttt tatttctgct gctgtcagga caccgatgaa
     3301 gacaggacta ccgtgctgta cttgcaggac tgcgcccagc aggcagggca ggagtcgcgg
     3361 tttatctaca ttgaagatct cggtttgggc gtcggcggtg tactgaccga tcttgatgat
     3421 aatgtcatcc agcgtgcatt taagctgtat ccgctggagt ggatgatgcg tgacgataac
     3481 ggtccgctgc tgcgcaagcg tcgcgagcaa tgggtggagc cgttatggaa aagtatcttg
     3541 agtaataaag ggctaatgcc gctgctttgg cgcttcttcc ctggtcatcc taaccttctt
     3601 gcatcctggt ttgagggcga aaaaccgcag attgccgctg gcgaaagcta tgtgcgtaaa
     3661 ccgatctact cgcgcgaagg cggaaacgtc accattttcg acggtcagaa taacgtcgtt
     3721 gaccacgctg atggtgatta cgccgatgaa ccgatgatct accaggcgtt tcaacctctg
     3781 ccgcgatttg gcgatagcta cacactcatc ggtagctgga ttgtcgatga tgaagcgtgc
     3841 ggaatgggga tccgtgaaga taacacgctg atcaccaaag acacctcacg tttcgttccg
     3901 cattacattg ctggataaga atgttttagc aatctctttc tgtcaggaat ccatggcagt
     3961 gaccatacta agggtgactg ccattgatgg agggagacac agtgcactgg caaactcaca
     4021 ccgtttttaa tcaacctata ccattaaata acagcaactt atacctgtct gatggcgcgc
     4081 tctgtgaagc ggtaacgcgt gaaggtgctg gctgggatag cgattttcta gccagtattg
     4141 gtcagcagtt aggaacggct gaatctcttg aactggggcg gctggcgaat gtgaatccgc
     4201 ctgaattatt gcgctacgat gcgcaaggac gccgtctgga cgatgtgcgt tttcaccccg
     4261 cctggcacct gctgatgcag gcgttatgta ccaatcgggt gcacaatctt gcctgggaag
     4321 aagacgctcg ctccggcgca tttgtggcgc gcgcggcgcg ttttatgtta catgcgcagg
     4381 ttgaggctgg gtcgttatgt ccaataacga tgacctttgc cgccacgcca ttgttgttac
     4441 agatgttacc cacgccgttt caggactgga ccacgccgct gctgagcgat cgctacgatt
     4501 ctcacttatt gccaggtggg caaaaacgcg gtttgttgat tggcatggga atgacggaaa
     4561 agcagggcgg ttccgatgtc atgagcaaca ccacccgcgc agagcgcctg gaagatggct
     4621 cttatcggct ggtggggcat aaatggtttt tctcggtgcc gcaaagcgat gcgcatctgg
     4681 tgctggcgca gactacgggc ggtttgtcct gcttttttgt gccgcgcttt ttgcctgacg
     4741 ggcaacgcaa cgcgattcgc ctcgagcggc tgaaagataa gctgggtaat cgctctaacg
     4801 ccagctgcga agtggagttt caggatgcca ttggctggtt gttggggcag gaaggggaag
     4861 gaattcgtct gatcctgaaa atgggtggga tgacgcgttt tgattgcgcc ctgggtagcc
     4921 atgccatgat gcgccgtgca ttttcgctgg cgatttatca tgcacatcaa cgccatgttt
     4981 ttggtaatcc attgatccaa cagcccctta tgcgtcatgt cttaagtcgc atggcacttc
     5041 agcttgaagg gcaaacggcg ttgctgtttc gtcttgcgcg agcgtgggac cggcgtgccg
     5101 atgccaaaga agccctgtgg gcgcgtttat ttacgcctgc ggcaaaattt gtgatctgca
     5161 aaagaggtat gccgtttgtg gccgaagcga tggaggtgct gggcggcatt ggttattgcg
     5221 aggagagcga gctgccgcgg ctttaccggg agatgccggt aaacagtatt tgggaaggtt
     5281 ccggcaatat tatgtgcctg gatgtgctgc gcgttctcaa taagcaagcg ggcgtatacg
     5341 acttattgtc ggaagcgttt gtggaagtga aagggcagga tcgctatttt gatcgcgcgg
     5401 ttcgtcgttt acagcagcag ctgcgcaagc cagctgaaga actggggcga gagattactc
     5461 atcagctatt cctgctgggc tgcggtgcgc aaatgttgaa atatgcctct ccgccaatgg
     5521 cgcaggcgtg gtgtcaggtg atgttagata cgcgcggcgg cgtacggttg tcagagcaga
     5581 tccagaatga tttattgctg cgggcgacgg ggggagtgtg tgtgtaagcg tatacgactg
     5641 atgcgacgct ggtttcgatt aactcaatga aatatgcgaa cattgtaggc cggacaaggc
     5701 gctcgcgccg catccggcat tgttcatttg ctgtatataa cactcatgca tacagtatcg
     5761 cctgtacgcg ccagttatca ggctgctggt cttcgtacat ggtaataatg cggtaccatg
     5821 acgcaccttg ttcatcggcc tttaacgcca cgatacgttc cacgtcctgc ggatcccctg
     5881 aaatattgct aactgagatc tgacctattt cattcaggcc cgtcacgcag tcagcactgg
     5941 cgaattctgc agactgtgcc gtggcgctcg caggataagt cactaattgc agcgagggtg
     6001 aggcaagtaa ttgtttcatc gtcagctcca tttttcgttt ccccacatgt tgcagggcat
     6061 tccatcgcgg taataaacgc ccaggcttcc agccggtaat ttattgtgca caggtaaacg
     6121 catagggacg caaagcaatg taaagctgac tatgtaagtt gtctgattta tttacggtac
     6181 aaaatggctt gtgaatacca ctgtcctgtc acgatggttt cgtccaccat gacgacaacg
     6241 taataatcag cttttgcagc gacagctttt gctctgattt ctgctaatgc gtcatccgga
     6301 gaaccccgaa ccatcgtgct tacgctacct attcgctgta acccttgcgt ctggtcgcgg
     6361 cgaatctctt gcggatggtt cgttactggc ggtgctggct gtggcgtacc ttgcagagcg
     6421 ctacaggcac ttaacatcaa caccaataat aaactggcaa accggtaaat aacgctatta
     6481 cgtttcctgc taaccatagt gtagtgcctt attaatttaa ctttggggga atgttcccga
     6541 attgttacct ggtacgcgat tttcgaatga aaacgtgacg aaagcgtaac ccacatctca
     6601 acattatgaa ctaataccgc cagcagaaac ttgtgctaag gatttcttca tgtcatacca
     6661 gacaaaaagg agagacagat gattgaaata gaatcacgcg agctggcaga tattcccgtt
     6721 cttcatgctt atcctgtcgg gcaaaaagat accccgttac cgtgcgtaat tttttatcac
     6781 ggctttactt catccagcct ggtgtatagc tattttgccg ttgcgctggc gcaggctggt
     6841 ttgcgggtga tcatgccgga tgcgcccgat cacggtagcc gttttagtag cgacgaagcg
     6901 cggcggttaa atcaattctg gcaaatcttg ctacaaagta tgcaggaatt cactacttta
     6961 cgtgcggcaa tagccgaaga aaactggctg cttgatgacc gtctggcagt cggtggcgcg
     7021 tcgatgggcg cgatgacggc actgggaatt accactcgcc accccacggt gaaatgtacc
     7081 gccagcatga tgggatcggg ctattttaca tcgctcgccc gttcactgtt tccaccgctg
     7141 atacctgaaa cggcagcaca gcagaatgaa ttcaataaca ttatcgcgcc gctggcagag
     7201 tgggaagcga caaaccacct ggaacaactt ggggacagac ctctactgct gtggcatggc
     7261 ctcgacgatg acgttgtgcc tgccgacgaa tcactacgtt tgcagcaggc cttaagcgag
     7321 acgggacggg ataaactgct aacctgttca tggcagccag gcgtgcgtca ccgcattacg
     7381 cctgaggcgt tagatgctgc cgtgacattt ttccgccagc atctttaaac acgcagaatg
     7441 ctgacccctt gcgcttccag ttgttgcagg atttccgggt tagcattttt gccggtgatc
     7501 agcatatcga tttgatcggc acggctaaaa agcattcccg cgcgttcgcc aatcttactg
     7561 ctatcaacca gtaccaccag tttccctacc acgctcagca tcttctgctc tgccattgct
     7621 gtcagcatat cggttttata caacccttct gcggtcagcc cttttccgct ggtaaacatc
     7681 cagtgcccgg catagagact gttttcgctg ccctgcgggc ttaaagtgat ggactgactt
     7741 ttgttgtact gtccacccat aatgatcacg ctgtcatgtt cttgatcgat caggtaattt
     7801 gccagcggta gataattagt gatgatttgc actggcttgc cacacatttc ccgcccaagc
     7861 agaaacgcgg tggagccgca gttgatgact acgctttcgc ccggattaac cagctgcgag
     7921 gctgctttag cgatacgtac tttttcatcg tgattctgcg cctgatgcag attcatcggc
     7981 gtccagcgcg ggcgctgctg ggtaatagct tctgcgccat tgcgcacttt tttcagtttg
     8041 ccgctttcgt caagtttatt gatatcgcgt cgcgcagtgg caggcgaaat tcccagacgc
     8101 tcaacgactt tctcaacggt cacaaagccc aattgtgcga gcatttccag gaggatttga
     8161 tgtctttgtg cttcagtcat gagctattcc gataaaattt gattttttta gatgatattt
     8221 gaaatagcca ggaaatacta cgccggatag cgcgaacttc tccacgctat ccggtataaa
     8281 cgacagatta caggaatgac ttgaacggca gatccggttc aatagtgaag caatcatcga
     8341 aaccgcgcgg atagtggtac tcgaagttgt ctttatccag cggccaggta aatttgccgc
     8401 caacctgcca gataaacggc ttgaagccat acttcagacg atcttttttc atctcccaca
     8461 gcacgcggat ctcttgcgga tcggcctgga agtttgacca gatgtcgtgg tggaacggga
     8521 taaccacttt cgcattcagg gcttcaccca tacgcagcat atcggcgctg gtcattttgt
     8581 cggtaatacc gcgcgggttc tcaccatacg atcctaacgc cacatcgatc tgatgttcgt
     8641 taccgtgctt cgcataatag ttagagtagt gggagtcgcc gctgtgatac agggagccgc
     8701 caggcgtttt gaacagatag ttcaccgcgc gatcgtccat gccgtccggc agtacgccag
     8761 ccgctttttg atcggcaggc agggtgatca gtgcagtacg gtcgaaagcg tcaagcgcat
     8821 gaatttcaat gtcttttact tttacaacat cgcccggttt gaccacgatg caacgctctt
     8881 tcggtacgcc ccagccaatc cacaaatcaa cgcaggtttt cggtccaatg aatggtacgt
     8941 catcagcaca gttctgcatc acggcagcag cgacgttaac gtcgatatga tcgttgtgat
     9001 cgtgagtcgc cagtaccgcg tcgatctggc gaatcgcaaa cggatcaaga acaaacgggg
     9061 tggtgcgtag gtttggctgc agttttttca cgccagccat gcgctgcatc tggtgaccct
     9121 gtttcattaa cgggttaccg tgactttgtt tgccagtgcc gcaccagaaa tcaacgcaaa
     9181 cgttggtgcc accttctgat ttcaaccaga tcccggtgca gcctagccac cacatcgcaa
     9241 atgtgccagg ggcgacctgc tcttgttcaa tttcttcatt cagccagcta ccccactccg
     9301 ggaaagtgct caggatccag gattctcggg tgatactttt cactttactc atcgccattt
     9361 accttcatga tagttcaatt cgaatcaata tgtgattggt tttgattaat cctgacacta
     9421 ttttttcagg aaggcaatga ccattttttg actttttgcc agggaagttg ttgttgattt
     9481 tgagtgtgga aagatttaat ggaatgtgta gttaaattaa ctaaatgaat ttaaataaat
     9541 aattgtttcg ttgtgtgaat cccactctat ccgtgtggaa ttatttgcgg gtcgcgtcac
     9601 atttaatcat aaataatctt gttgtgatta cttttgaaaa ttagagtgag tgcacaacat
     9661 tccgggtgtg cggaataccc ggttacctct tcttcaggag atcgttatgg agatcctcta
     9721 caacatcttt accgtgtttt ttaaccaggt catgaccaat gccccgttgt tgctgggtat
     9781 tgtgacctgt ctgggctaca tcctactgcg caaaagtgtc agcgttatta ttaaaggcac
     9841 gattaaaacc ataattggtt tcatgttgtt gcaggcaggg tccggcatcc tcaccagcac
     9901 cttcaaaccg gtggtggcga aaatgtccga agtctacggc attaacggcg caatttccga
     9961 tacttacgcg tcgatgatgg caaccatcga ccgcatgggc gatgcctata gctgggtagg
    10021 ttacgcggtg ttgttagcgc tggcgctgaa catctgttac gtgctgctgc gtcgcattac
    10081 cggtattcgt accatcatgc tgactggtta tatcatgttc cagcaggcgg ggctgattgc
    10141 cgttacgttg tttattttcg gctactccat gtggacaacc attatctgca cggcgattct
    10201 ggtttcgctc tactggggca ttacctccaa catgatgtac aagccgactc aggaagtgac
    10261 ggacggctgc ggtttctcca tcggtcacca gcaacagttt gcatcatgga ttgcctataa
    10321 agttgcgccg ttcctcggca aaaaagagga aagcgttgaa gacctcaaac tgccaggctg
    10381 gctgaatatt ttccacgaca acatcgtctc cacggcgatt gtgatgacca tcttctttgg
    10441 tgccattctg ctctccttcg gtatcgacac cgtgcaggcg atggcaggca aagtgaactg
    10501 gacggtgtac atcctgcaaa ctggtttctc cttcgcggtg gcgatcttca tcatcactca
    10561 gggcgtgcgc atgtttgtgg cggaactctc tgaagcattt aacggtatct cccagcgcct
    10621 gatccctggt gcggttctgg cgattgactg tgcggctatc tatagcttcg cgccgaacgc
    10681 cgtggtttgg ggctttatgt ggggcaccat cggtcagctg attgcggttg gcatcctggt
    10741 cgcctgcggt tcctcgatcc tgattattcc cggctttatc ccgatgttct tctccaacgc
    10801 caccatcggc gtgttcgcta accacttcgg cggctggcgt gcggcgctga agatatgtct
    10861 ggtgatgggg atgattgaaa tctttggctg cgtctgggcg gtgaaactca ccggtatgag
    10921 cgcctggatg ggcatggcgg actggtcgat tctggcaccg ccgatgatgc agggcttctt
    10981 ctccatcggt atcgccttta tggccgtcat cattgtaatt gcactggctt atatgttctt
    11041 cgctggccgc gcactgcgcg cagaagaaga tgcagaaaaa caactggcag aacagtctgc
    11101 ttaataagga gttttgatta tgaccgtacg tattctggct gtgtgtggca acggacaagg
    11161 cagttccatg atcatgaaga tgaaagtgga ccagttttta acccaatcaa acattgacca
    11221 cacggtaaat agctgcgcgg ttggcgaata caaaagcgag ttgagcggcg cggatatcat
    11281 catcgcttct acgcacattg cgggcgaaat caccgtgacc ggcaacaaat acgtggttgg
    11341 cgtgcgcaac atgctctctc ctgccgactt tggcccgaaa ctgctggaag tgatcaaagc
    11401 gcatttcccg caggatgtga agtaaggacg cgccatgaaa ttacgtgatt cgctggcgga
    11461 gaataaatcc atccgcctgc aggctgaagc tgagacatgg caggacgcag taaaaatcgg
    11521 cgttgacctg ctggtggcgg cggatgttgt cgagccgcgt tactaccagg cgattctgga
    11581 tgcggttgaa cagcacggtc cgtactttgt gctcgcacca ggcctggcaa tgccgcacgg
    11641 tcgcccggaa gagggcgtta agaaaaccgg ttttgcgctg gtgacgctga aaaagccgct
    11701 ggagttcaac cacgaagata acgacccggt ggatatcctc atcaccatgg cggcggtcga
    11761 tgccaacacg caccaggaag tgggcatcat gcagatcgtc aacctgtttg aagatgaaga
    11821 aaatttcgac cgtttacgtg cctgccgtac cgagcaggaa gtactggatc tcattgaccg
    11881 caccaacgcg gcagcttaag aaggaattga agatgtcatt accgatgttg caagtcgcgc
    11941 tggacaacca gactatggat agcgcctacg aaaccactcg cctgattgcc gaagaagtcg
    12001 acattatcga agtgggtacc attctgtgcg tgggcgaagg cgtgcgtgcg gttcgtgacc
    12061 tgaaagcgct ctacccgcac aaaatcgtac tggcagacgc taaaattgct gatgcgggca
    12121 aaatcctttc gcgtatgtgc ttcgaagcca acgctgactg ggtgacggta atttgctgtg
    12181 cggatatcaa caccgccaaa ggcgcgctgg acgtagcaaa agagtttaac ggcgacgtgc
    12241 agatcgaatt gaccggctac tggacctggg aacaggcgca gcagtggcgt gacgcaggca
    12301 ttcagcaggt ggtttatcac cgcagccgtg acgcgcaggc cgcaggcgta gcgtggggcg
    12361 aagcggacat taccgcgatc aaacgtcttt ccgatatggg cttcaaagtc accgtcaccg
    12421 gtggcctggc gctggaagat ctgccgctgt tcaaaggtat tccgattcat gtgtttatcg
    12481 ctggtcgcag catccgtgat gccgcttctc cggttgaagc tgcacgtcag ttcaaacgtt
    12541 ccatcgccga actgtggggc taaggagcgg atatgttgtc caaacaaatc ccgcttggca
    12601 tctatgaaaa agcgctcccc gccggggagt gctggctgga acgcctgcaa ctggcaaaaa
    12661 cgttgggctt cgattttgtc gagatgtcgg tagatgaaac ggacgagcgc ctgtcgcgcc
    12721 tcgactggag ccgcgagcag cgtctggcgc tggtgaatgc gattgttgaa accggcgtgc
    12781 gcgtgccgtc catgtgcctt tctgctcatc gtcgtttccc gctgggcagt gaagatgacg
    12841 cggtgcgggc gcaggggctg gagattatgc gtaaagctat ccagttcgcc caggacgtcg
    12901 gcattcgcgt gatccagctg gcgggctatg acgtttacta tcaggaagcc aataacgaaa
    12961 cgcgtcgtcg tttccgtgac ggcctgaaag agagcgttga gatggcgagc cgcgcgcagg
    13021 tgacgctggc gatggagatc atggattatc cgttgatgaa ctccatcagc aaggcgctgg
    13081 gttacgcgca ctatctcaac aacccgtggt tccagctcta cccggatatc ggcaacctgt
    13141 cggcgtggga caacgatgtg cagatggagt tgcaggccgg aatcgggcat atcgtcgcgg
    13201 tacatgtgaa agacaccaaa cctggcgtct tcaaaaacgt gccgtttggc gaaggtgtag
    13261 tggatttcga acgttgtttc gaaacgctca aacagagtgg ctattgcggg ccgtacctga
    13321 ttgagatgtg gagcgaaacg gcggaagacc cggcggcaga agtggcgaaa gcgcgtgatt
    13381 gggtgaaagc gcgcatggcg aaagcgggca tggtggaggc ggcataatgc aaaagctaaa
    13441 acagcaggta tttgaagcca acatggatct gccgcgctac gggctggtga cctttacctg
    13501 gggcaacgtc agcgctatcg accgcgaacg cgggctggtg gtgatcaagc ccagcggcgt
    13561 cgcctacgaa accatgaaag cagacgatat ggtggtggtc gatatgagcg gtaaggtggt
    13621 ggaaggggag tatcgcccat cttccgacac cgcgacgcat cttgaactct accgtcgtta
    13681 tccgtcgctt ggcggcattg tccataccca ctccactcat gctaccgcat gggcgcaggc
    13741 ggggctggcg atcccggcgt taggcaccac gcacgctgac tacttctttg gtgacatccc
    13801 gtgtacgcgt ggattaagcg aagaagaggt gcagggtgag tatgaactga acactggcaa
    13861 agtgattatc gaaacgctgg gcaacgccga gccgctgcat acgccgggaa ttgtggtgta
    13921 tcagcacgga ccgttcgcct gggggaaaga tgcccacgac gcggtgcata acgcggtagt
    13981 gatggaagaa gtggcgaaaa tggcgtggat tgcgcgcagt attaatccac aactcaatca
    14041 catcgacagc ttcctgatga ataaacactt catgcgtaag catggtccta acgcttatta
    14101 cgggcagaag taaaacacgc gctgcggaaa tttccttctt cgggagataa ctggtctaat
    14161 tccgcagccg tttttcaaaa aaagccccct gcgaaggggg caaagcaaac tatggcaatg
    14221 tttcgttggt tatacctggt gctagcgata aatatccgcg ctggcgtgca tattgccgtt
    14281 actccccggt tcccgcatca gaattacgtg gtagtacgtt gcgccctgcg catctgtttc
    14341 ttcatttaat gcctgacgtg cttcactttc agtggcgaaa ttatgattga tataaatcac
    14401 gcctaagctt tgcacatcgt ccatatttct ggcctggtgg ttattgattt caatggctgc
    14461 ccatgtattt gcacttagca aaagcacagc cagaagggct aaaacacgac tgaacataga
    14521 tacctcctca acggctgact ttgtgtgctc tcctctgtga tgatcttctg atttaatttt
    14581 aatcaatgat aaagaagttg atggtgacca tttctgatgc agttgttcga aaaaacactg
    14641 tgacggaatg tgatgaactt caaatcaggg agttagttct tttgagcgaa agatgagatt
    14701 tatttgtcgg ctggccttga gtttagcggg gcatacaagt actataacgc gtcatttttc
    14761 agccgacctt taacacgttc cttgcctccc cgggattcgg ctgacccaga caggaggctg
    14821 aataatccgt aaggagcaat tcgatgcgtc attacgaaat cgtttttatg gtccatcctg
    14881 accagagcga acaggttccg ggcatgatcg agcgctacac tgctgccatc actggtgcag
    14941 aaggcaagat ccaccgtctg gaagactggg gccgccgtca gctggcttac ccgatcaaca
    15001 aactgcacaa agcacactac gttctgatga acgttgaagc tccgcaggaa gtgatcgatg
    15061 agctggaaac taccttccgc ttcaacgatg ccgttatccg cagcatggtt atgcgtacca
    15121 agcacgctgt taccgaagca tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc
    15181 gcgatgattt cgcaaacgaa accgctgatg atgctgaagc tggggattct gaagagtaat
    15241 ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg
    15301 tcagtccatc aggaattcct cactgccagt tcgtgcttga gcatcgttct gtgcaggagg
    15361 aagccggctt tcaccggcag gcatggtgtc aaatgcccgt tattgttagc ggacacgaaa
    15421 accaggccat tactcacagt ataacggtcg gcagtcgcat aaccgttcag gggttcattt
    15481 catgccacaa ggcaaagaac ggactgagca aaatggtttt gcatgccgag cagattgaat
    15541 tgatagattc tggagactag ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt
    15601 ttcaccgcgg aaggcgttca agagatcgac tataaagata tcgctacgct gaaaaactac
    15661 atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag
    15721 cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc gtacactgat
    15781 cgccatcagt aatcggtcac ggtccattaa tacgactttg agaggataag gtaatgcaag
    15841 ttattctgct tgataaagta gcaaacctgg gtagcctggg tgatcaggta aacgttaaag
    15901 cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc tgttccagct accaagaaaa
    15961 acattgaatt cttcgaagca cgtcgcgctg aactggaagc taaactggct gaagttctgg
    16021 cagctgctaa tgctcgcgct gagaaaatca atgcactgga aactgttacc atcgcgtcta
    16081 aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg
    16141 taactgcagc tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc
    16201 gtaccactgg cgaacacgaa gtgagcttcc aggttcacag cgaagtattc gcgaaagtga
    16261 tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg ccgaccattg
    16321 gtcggcgttt tgctttctat ttttcgtcag gtattagttt cgcaagtaga tccattcacc
    16381 attctcttgt gtccataagt caggatggtt tgctggcgtt agatgcatac gatcactcct
    16441 tatgtctcgg tcctgcggca ctcgctcgaa acctaaaccc gagtagattg ctgctgaatt
    16501 tgataaggaa cgtagaccaa ttttcaaatc atggggtaaa tcagaatacg cgtttagcaa
    16561 tgcagcaatg atgaatccga gggcattaga aacgcctgta acagtgcgaa gttggtcgat
    16621 ataaagatcg attgttggta gttcagatga ccagcaatta ggcatttggg caggaagggt
    16681 tacaacgtat cctatcgttt ggccggtata gtctggagta ccataaccag gtcccaaaac
    16741 cacttcgtgt attgtgtatt tactgttggg ttcgaaggat tcgacatctt gcaataaata
    16801 tttcagttct gcggttaacc tcgcacccgc gtgagcttta ttgtatttct gggcgtgaat
    16861 attatctaat gtagctttat ggttccatcc aaaagaatta tcaccattca ttgttactcc
    16921 atcgtaagca ttacacttcg ttgctaactt attgaatgct tgccagtcag agagatctag
    16981 tttgtggatc tcaacagtac gtctgccatc gcaattatga tctcttccac gatttggcag
    17041 taatgtagca cgtgcatatt gatgaaattt taattttact ggtttgtcgg tggacgaaaa
    17101 atggggtgaa tatgcaggaa atgaataaat agtcgttggt aatgtcattg taatgttacc
    17161 tgtttaatgt ttttataaat atcgcagctg atgttcacag aaaaagaatc gatattaata
    17221 aaaataagtt tgttttctga agtagtgaaa atagcggatg gtgaaagata ttaatatcaa
    17281 aaatcgctca ttatggatat aacgatgccc ccggggagaa aatgacttcc acgccttcca
    17341 ccatacattc ttgttgtgcg gtatgtaatt attctacgct gttggtatcg gtgcgtttgt
    17401 taatcatctg gttaaccagc cacaaaaagc cattgagaaa attattgatt ttacatgggg
    17461 ttattatatt gctaaccctt ggtttttaaa aattgtgcat agtgaaaatc aaagtaaagg
    17521 tgtgcattat gcaaaatcac agcgtctctt agaaattaac catgcgcact tgcagttaat
    17581 ggaatcctta ttggatgaag ggaagaaata caatattttt aagccaggta tcgacccatt
    17641 gcaggtgaat attaatattg ctgcgcttgg gggatattat ttgatcaacc agcatacgct
    17701 tggcctggtt tatctggtgc gccgtaaaac cccgtccttc agggcgggga tataaggcgc
    17761 ggttttccac ctgaccaggt gtttgctgtt gttcaatgta ttggcggatg acggatattg
    17821 gcgcacctcc gcagctattg gcaaaatagc caggactcca caaaacgcct ttgtagtaat
    17881 acctgactgc aatatctggt cgatctcgtc gcagtaacct acccgatacg cctttgaggc
    17941 tgtttaccag actggatatc gccagtttgg gaggatagct aattaacaaa tggacgtgat
    18001 ctggttcgcc atccatttca accagttcag cttcaaaatc agcacataca tttgaaaagt
    18061 aagtgcgtag tttttctgtc gcgtcatggt caaaaatctg gcgtcggtat ttggtgacaa
    18121 agaccaggtg aacatgcatc aggaaaacac aatgccttcc acgccggata tcggtttctt
    18181 ttttcacaga ccaaaatata atttgacctg tggaacgact acaggcattt aaattccagt
    18241 taagacccgg tggtcaacag gagcgtgaaa tgaggcgctt cgcaggcgca tgtcgtttcg
    18301 ttttcaatcg tgctctggca cgtcagaatg agaatcatga ggccggtaat aaatacatcc
    18361 cttacgggaa aatggcttcc tggctggttg agtggaaaaa cgccactgaa acgcaatggc
    18421 ttaaagattc gccctcacag ccattgcaac agtcactgaa agaccttgag cgggcttaca
    18481 aaaacttctt ccagaatcgg gctgcttttc cccgattcaa aaagcgggga cagaatgatg
    18541 cattccgcta cccgcagggc gttaagctcg atcaggaaaa cagccgtatt tttctgccga
    18601 aacttggctg gatgcgctac cggaatagcc gtcaggtcac gggagttgtg aaaaatgtca
    18661 ctgtcagcca gtcctgcggt aagtggtaca tcagtattca gacagaaagt gaagtatcca
    18721 ctccggttca cccttcagca tcaatggtcg ggctggatgc tggcgtggct aaactcgcca
    18781 cgctgtcaga tggcacagtc tttgagcctg taaacagttt tcagaaaaac cagaagacgc
    18841 tggcgagact tcagcgacag ttaagccgca gggtcaaatt cagcaacaac tggcagaagc
    18901 agaaacgcaa aatacagcga ctgcattccc gtatcgcaaa tatccgcagg gactaccttc
    18961 ataaagtcac aacgaccgtc agcaaaaacc acgcaatgat agtcattgag gatttgaagg
    19021 tcagcaacat gtcaaagtca gcagcgggta cggtcagcca gccggggcgc aatgtccggg
    19081 caaaatcagg tttaaaccgt tcgatactgg atcagggctg gtatgaaatg cgccgccagc
    19141 ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc tgttccgcca gcgtacacaa
    19201 gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa tcgcctgtca caaagtaaat
    19261 tcagatgcca ggtatgtgga tatacagcga acgccgatgt aaacggcgct cgtaacattt
    19321 tagcggcggg gcacgccgtt cttgcctgtg gagagatggt gcagtcaggc cgctcgttga
    19381 agcaggaacc caccgaaatg attcaggcga cagcctgaac gaagcaggga tccacgtcct
    19441 tcagggcgtg gaggatgtca acatatcagt atggtttccc cccaggcact ggaagccaga
    19501 cgtaaggtca tcaaagagac aatccttagc tggcttttgg ttgacccttc atctaccgcg
    19561 cacgaataaa actgccatcc ggctggcggg taaacaggac ctgttgatta ttccccgtat
    19621 caatggttaa gcccgtcacc acgccgctgg cgttctggcg gattttcacc atttgcccgt
    19681 tttgcaaatt actcagcggc ttacccgcgc cttctacctg cgccattgca tagacatcgg
    19741 tagcgggcag tccgtggtcg cggaacagct gcgccatcgt tttaccgggt tcgacacggt
    19801 aagagcgcca ttgattatca attccgctat ctggctggaa tggttgcgtt tgggtagttt
    19861 gcggctgctc ttctggctgg ccttcctgta taggttctgg agcgaccgga gccacctgat
    19921 caggatcatt ttgtggggtg actaattgcg cccgcagctg ttcttctgtc ggcggttgtg
    19981 attgcgactg aatatctaac tgcgcttcac gcgtcaccac cggcgcgttg ggcgtatcat
    20041 cagatggaag cagaaatccg accaccaata caatggcggc aataatgatc ccccgacgat
    20101 gcatcggcgg cagcgggtcc atgaagcgaa aattgtccgg cgcgtgccag actttctcca
    20161 gggttggttt tagttcaaag cgcccgggca tggtttcctc ctgctccgcg tcttgttcct
    20221 caatcatagc ctatgaataa gctaacgcta tgatgtccgt ggtaaacccg cctttattat
    20281 attcatacgg gatattgcta ttgtttcttt ttccccggga tttgtcatca ttcctgcgac
    20341 aaagttttac ccaaagaagt gtggctgata tgctgcccgc tacttttata ccctaagaaa
    20401 ggaaatacga tgaccacccc aacttttgac accatcgaag cgcaagcaag ctacggcatt
    20461 ggtttgcagg tagggcaaca actgagtgaa tctggcctgg aagggctgct gccagaagca
    20521 ctggttgcag gtattgccga tgcgctggaa ggcaaacatc cggctgttcc ggttgatgtg
    20581 gtgcatcgcg cgctgcgtga aatccacgag cgcgccgatg ccgttcgtcg tcagcgtttc
    20641 caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa acgccaaaaa agaaggtgtg
    20701 aatagcaccg aatctggcct gcaattccgc gtgatcaacc agggtgaagg cgcaattccg
    20761 gcacgtaccg accgcgttcg tgttcattac accggtaaac tgatcgacgg caccgtgttt
    20821 gacagctccg ttgctcgtgg tgaacccgct gaatttccgg ttaatggcgt gatccctggc
    20881 tggattgaag cactgactct gatgccggta ggttctaaat gggaactgac tatcccgcag
    20941 gaactggcat atggcgagcg cggcgcaggc gcatccatcc ctccgttcag caccctggtg
    21001 tttgaagtcg aactgctgga aatcctctaa gcagcgcatt ctgttcccct cgaaccagag
    21061 gggagcaggc attcagcaat aaacccttca gtttgccaaa cggcgctatt ttgtgttgca
    21121 aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt tatcaccgtg atatgacact
    21181 cactttaaac ataaaattaa cattagatct aaatcttagt attcatcccg cgtattgtta
    21241 cctaatatcg atgagtcccg atacagattc gtcgtatcat aggctgacta aaggccgtag
    21301 agcctgaaca acacagacag gtacaggaag aaaaaaacat ggtagatcag gtaaaagtcg
    21361 ttgccgatga tcaggctccg gctgaacagt cgctacggcg caatctcaca aaccgacata
    21421 ttcagcttat tgccattggc ggtgccattg gtacggggtt gtttatgggg tctggcaaaa
    21481 cgattagcct tgccgggccg tcgatcattt tcgtttatat gatcatcggt tttatgctct
    21541 ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa tctggaatac aaatctttta
    21601 gtgacttcgc ttccgattta ctcgggccgt gggcaggata tttcaccggc tggacttact
    21661 ggttctgctg ggttgtaacc ggtatggcag acgtggtggc gatcacggct tatgctcagt
    21721 tctggttccc cgatctctcc gactgggtcg cctcgctggc ggtgatagtg ctgctgctga
    21781 cgctcaatct cgccaccgtg aaaatgttcg gtgagatgga gttctggttt gcgatgatca
    21841 aaatcgtcgc cattgtgtcg ctgattgtcg tcggcctggt tatggtggcg atgcactttc
    21901 agtcaccgac tggtgtggaa gcgtcattcg cgcatttgtg gaatgatggc ggctggttcc
    21961 cgaaaggttt aagtggcttc tttgccggat tccagatagc ggttttcgct ttcgtaggga
    22021 ttgagctggt aggtacaacc gctgcggaaa ccaaagatcc ggagaaatca ctgccgcgtg
    22081 caattaactc tattccgatc cgtatcatta tgttctacgt cttcgcgctg attgtaatta
    22141 tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag cccgtttgtt gaactgttcg
    22201 tgttggtggg tttgcctgcg gctgccagcg tgatcaactt tgtggtgctg acctctgcgg
    22261 cgtcttccgc taacagcggc gtcttctcta ccagccgtat gctgtttggc ctggcccagg
    22321 aaggtgttgc accgaaagcg ttcgctaaac tctctaagcg cgcagtaccc gcgaaagggc
    22381 tgaccttctc ctgcatctgt ctgctcggcg gcgtggtgat gctgtatgtg aatcccagcg
    22441 tgattggcgc gttcacgatg atcacgacgg tttccgcgat tctgtttatg ttcgtctgga
    22501 cgattatcct ttgctcgtac ctggtgtatc gcaaacagcg tcctcatctg catgagaagt
    22561 cgatctacaa gatgccgctc ggcaagctga tgtgctgggt atgtatggcg ttctttgtgt
    22621 tcgtgatcgt gctgctgaca ctggaagatg acactcgcca ggcgctgctg gttaccccgc
    22681 tgtggtttat cgcgctgggg ttgggctggc tgtttattgg taaaaaacgc atggcgaagt
    22741 aatgccgcta aggcacctgc gggtgtcttt caggctgcaa aattgcctga tgcgctacgc
    22801 ttatcaggcc tacagaacct ctgcaatata ttgagtttgc gtgcttttgt aggccgggta
    22861 aggcgtttac gccgcatccg gcatgaacaa agcacacttt gtcagcaatc tcctttgtct
    22921 ttatacattc ctaaaaatac ttcccgtcat cgacagctct caggcgcggg cttaccgtgg
    22981 atctttgcca ctcattgcag agttcactat ggctaagcac agttggttat taatcgccgc
    23041 attacctctc tccatttcac cttcctgggg cgcagatttt tattaccgcc agcaggagaa
    23101 aggcacggtt tatgttgccg aacagaaggg agaaaaggat gagatcctct ccgaactgcc
    23161 gggcgttaat ttttcccgcc tctggcgtat tgccaattta gccaataacc aagaaacccg
    23221 cttactgtcc gattttaatc ccgataagtt cgattgcgat gatcggaata attgccagca
    23281 tacctggctc accgatggac gctctgttct ttggtctggc aaagtcctga aaaatccgcc
    23341 cggtgaacct aatgtcgatg ccgccagttt tcaggcattc ggtgctttcg ctgccgataa
    23401 acgcagcgtc tattttgacg gtcagcgtac cgatgataga tgaagtggat atgtatatag
    23461 ctgtgtctca gttgcaaaca aatatttatg taccgaaata aaagtatcaa tagacaatta
    23521 attaataaat caggagataa agtggctata ttacatccac aggagtgctg gttgctggaa
    23581 agaataatgt cgccggaata ttatcggcgt cgttttgagg gctggcagga gtttgtcgag
    23641 ctgtgcgagc gacaggtggc agagtggagc aagaccatgc cgctggatgt gcgccgtcgt
    23701 ccgctgtgcg agcagattga cgtcgtctgg ggcgggcgag tgttgcccaa tatccgcagt
    23761 actttaaagt cggtacagta tgactttatt cagctacagc agggggacct gcgggtactg
    23821 caatccgggg gcaatattag cagtgatatg aaaggactaa ttgattatcc gtcggactgg
    23881 atgtcactcg ccgcgcaaaa acagtatgac agactgaaat ggcgaggtgc acattataat
    23941 aatttaatca gacgtacctc gggcggatac tggtatgacg gcgaactgac ttattattat
    24001 gaggaaagtc tgcacggccc gctggccctg ccgatgcaat taccgttgta tgagctggat
    24061 agtagcgtat acctgcgtga ggatgactcg gtaacggtgg ctggcctgta tctgccggat
    24121 atcccagatg ccagcgccca actgttgtac cggagtgaac atattcccga ggcttggcag
    24181 gggcgggtac gaacgaagta tgtcaacgag gctgggatac aagaatatta ctgggaaagc
    24241 ggggcgtggg caaaatgcaa ttggaaacgt atacggcggg tggcaaaccg atttatcaat
    24301 gtgccgccgg agggattttt cccacagggc atgccggagg agttgtacaa ctggccacag
    24361 cgcgaagcgc agtatgttac agaccggcag cggatagcgg cctttagcgg tgaagcttgt
    24421 cctcatagcg gggagtggtc gatttttgta gaaggtagac aagcgacggt gacactggag
    24481 cagggagagc agatgccaga atggacggac aggaaaatgg agggggagta taaaagaggc
    24541 gagaaattcc atgtgctgtg gtcgctgatg aaccgccatg atggcgggtc ggtgtgggtg
    24601 gaagcgtagc ggtagctgga tgtacacgta aaaaccaggg aatgggtgat tttattctgg
    24661 cttatacagg agcgatgaat aagtaattat attaatgaaa aaaataagtt agtaaccgtt
    24721 gaaggaactt attattggct gaataatgaa ttttcccagt tcagtagata caaaagattg
    24781 tcccggagtg gtgaataact caaagcattt gaaaacgcac ctgattgtgt aaaccaggtg
    24841 cgttgatcga acaaattact cacccgccag cgcgcgtggg aacagtacat tgttttccag
    24901 gctgatgtga tccatcaggt catcaatcag ttcattaata ccgttataca tcgctttcca
    24961 ggtggtgcag gcttccggcg gcggtgtgac gttattggtg gtgtgtttaa tcacttccag
    25021 cagttcgccc gcttcatcgt gctcgctttc catcacgctg attggtccca ttgcctggct
    25081 gcccatgcct tgtttgatca tcgggaagag gatctgctct tctttcatca tgtggctgga
    25141 aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacgc tcggtttgtc
    25201 ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagct gctcgcggtg
    25261 acgatcgtgg tagcgcacga tgatatggtc aatgatttct gccagcgggg cgctacgcca
    25321 gtctttctca atcggttgtt cagcaagctt tgccagttca gcttcaatga cctcaacatc
    25381 cagttcttta cgtgccgccg cgcgcgccag cgtctgctta ccgccacagc agtaatccat
    25441 atcatattta cgaaacagag ccgaagcgcg agggatagag agcgccagtt cacctaaagg
    25501 ttggtcgcga taagccatag ctgatacctc attcttaata agataagatg tattttaaat
    25561 gcatctttaa ggcaaaagct ataaccctta cgtagcagag gcttatttaa ctcactgcca
    25621 gcgtgtcaac tttctgagac agattcagtt aaaggttttt ctggttttgc tctgaccgca
    25681 atgaccacgc caatcactaa cagcgcgata ccgctcagcg tcattagcgg cggcatttgc
    25741 tggcggagta aaaaggtgta cagcaaacct gccagcgttt cgaaaacaat cagcggcccg
    25801 agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc aacccatgag
    25861 caaagcacgg ctatcgcaac catcagacta ataaacacca gcggacgggg gccaaaaggt
    25921 aaggagaagt ccggcgtttg tatattcagc cagtaacagg cgacgagata gccgatgaga
    25981 gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga
    26041 ttttcccgca gccagcgggc gttgcgcagg gcataccatg cccagcagac cacggaaact
    26101 aacgctagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag tccgtggttt
    26161 aactcagcaa tattcacact cgccaggccg atgccaatac aaatcagtgc cggggcgagt
    26221 tttccccacg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa gacaggaatg
    26281 accaccggca gggtgccgat aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca
    26341 ctggcaaggc agaagtaata aatgaggttg cccatcatag tgagcatcaa ggcggtaagc
    26401 cagtcccgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat gggcagcgca
    26461 attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta ttccggtacg
    26521 atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc gtacagcacg
    26581 ccgctaatca ttactggcat ccattggtct gtcagaagaa agttcaggaa gcagacatga
    26641 ttgctcccgg catcccgtca ttattagaga ggatgcggga ggatgccact ttaattaact
    26701 agttattaac attaaaaaga tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa
    26761 tcaatttgct aagcgttttg ctgttatcaa acagaccacc tttcgatgcg ccaacgtcag
    26821 aatccgccag catatccgcc agtccgtcgg gcagtccgac gcttttcagt gccgcggcga
    26881 aatcggcttc gctcagattt tgataggtaa cctgtttgcc gctctgtttg gtcagctctg
    26941 ccgctaactg tgtcaatgtc caggcactat cgcccgccag ttcgtaaacc ttgccttcgt
    27001 gaccggcttc gctaatcacg cgtgccgcag ctgccgcata atctgcccgc gttgctgagg
    27061 cgattttgcc atcgcccgcc gcaccgataa atacgccgtg ttccagtgct gccggggcgc
    27121 tggcgaggta gttttcgctg taccagccgt tgcgcagcag ggtgtaaacg atgccagaat
    27181 cagccaacat tttctccgtc tcgatgtgct catcggcgag gccgagcggg gaggtatctg
    27241 catgtagcag gctggtataa gcgataaatt tcacaccagc cgcctttgcg gcattaataa
    27301 cattacgatg ctgcggggca cgttgaccca cttcgctgga agagatcagc agtagttttt
    27361 ccactccctg aagtgcagat gtcagtgcgg cttcatcgcc gtagtcagcc tgacgcacgg
    27421 taatgccttg tgctgccaga gcctgggctt ttgccggatt acgaacgata gccactattt
    27481 ggctgacagg aaccgttttc ttcaaggatt caataacata gtgaccaagt tggccagtgg
    27541 caccagtaat agcgatcatg ggaagtctcc atcgttttgt cttgtgttta aatacgctaa
    27601 cacctaaact tacttttagt aagtacgcac aaaaaggtaa gtatgaaatg agtcaggtta
    27661 gcctgtcgca acaactgaaa gagggtaatc tctttgcgga acagtgcccg tcgcgcgagg
    27721 tgttgaaaca cgtcaccagc cgttgggggg tgttgattct ggtggcgcta cgcgaaggta
    27781 ctcatcgctt tagcgacctg cggcgcaaaa tcggcggggt gagcgaaaag atgcttgcgc
    27841 agtcgttaca ggcgttagaa caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc
    27901 cgccgcatgt ggaatatagc ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg
    27961 cactggcgga ctggattgag ttgaatttgc ccgaggtgtt ggcggtgcgg gatgaacgtg
    28021 cggcataact gccggatgcg ctgagcttat ctggtctacg gtgcatggct tgtagggcgg
    28081 ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcggttgat gatttatagg
    28141 ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac ccgatatcat
    28201 cggtcgcgac tttattcatc ggatattgcc ctttctcttt aataaacgct gcggctttat
    28261 ctgacggaga agtttcgaaa cggatatcca gtttcttatc gccagctatc ggtgctaaac
    28321 gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca tcagcaatcc
    28381 acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg
    28441 taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg aacatggcgt
    28501 tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca ttggcattaa
    28561 tcatctggca ctcgccgtca taacgggcag gctgggtaac atcaatctga taattcacac
    28621 catcaatcac atcaaagtta taggtgcgga aaccatccca gttgatgagt gattgcggtt
    28681 tcgtgctgtt aggatcaatc tggttaaact gtccggcgga gcactccagc cactctttca
    28741 cctctttacc gctggctttc accacaatca gcgtattggg gtagagataa agatcggcgg
    28801 cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg tcatttttgc
    28861 gaccaccaac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc agatccggat
    28921 cgccctgaat gtaatgttcg acatacgctt tttgcgcgtt gttcactact tgcacggtcg
    28981 gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta ccgattggct
    29041 tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc agcttgctgt
    29101 cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgagct tccgctttcg
    29161 cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc accccaagat
    29221 gatcgcccca catgcccggc attaccgccg gaacaccatt cagcgtgcct ttggtgatat
    29281 cagccccttc gatatcagca aaatctttac ccgggaaaac ggcgtgagca tgaccaaaca
    29341 taatggcgtt aacgcccgga atttcactga ggtaataaac tgagttttcc gccattactt
    29401 tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgacatcg gcacctttct
    29461 cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc gtcacttttc
    29521 cggataaatt agctttatcc cagcccatga tttgcggcgg cacgacgcca atatagccaa
    29581 tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtatcttta attaaatacg
    29641 gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca taagggaatt
    29701 tcgctcccgc cagcgcattt ttcaggtaat ccagaccgta gttaaattca tgattgccga
    29761 gtgtaccgac cgtataatcc agcgtattca gcgccttata aaccggatga acatcacctg
    29821 cttttaatcc tttcgccgac atgtaatcgg ccagcggact cccctgaatc aaatcgccgt
    29881 tatcgaccag tacgctgttt ttcacttcat tgcgggcatc gttaatcagg cttgccgtac
    29941 gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc
    30001 tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc acactggcgg
    30061 caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc cttttatcat
    30121 cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg aagaatgcca
    30181 caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt aacgataggg
    30241 tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa tgttagatca
    30301 agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg acgggacgaa
    30361 accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg atattgccgc
    30421 tcacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg tcctttctga
    30481 agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc tggtagaccc
    30541 gctggatggt actaaagagt ttattaaacg caatggcgaa ttcaccgtta acattgcgct
    30601 cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta
    30661 cagcgcggca gaaggcaagg cctggaaaga agagtgcggt gtgcgcaagc agattcaggt
    30721 ccgcgatgcg cgcccgccgc tggtggttat cagccgttcc catgcagatg cggagctgaa
    30781 agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt cgctgaaatt
    30841 ctgcctggtg gcggaaggac aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg
    30901 ggacaccgcc gctggacatg ctgtggccgc agctgccgga gcgcacgttc acgactggca
    30961 gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatcctgggt tcagagtgtc
    31021 tatttattaa ttaatttatg cagcaggtcc atcacttgct gcacctcttc ctgagtaagc
    31081 gccccgtctt tggcccattg cacgcgaccg tctttatcca gcaccactac agcggaactt
    31141 ttttcatcca gctgccaggc accgcgtgcg acgccattgc tatcaacaat aaactgcgac
    31201 cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc ggaacccgga
    31261 attgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg taacttcgct
    31321 gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg accagcaata
    31381 tgttgcagta ctcgcacttt tcccactaac tgcgcgctgt tccaggtttt gtagctaaac
    31441 tgatctttat caagcaccaa ctcgccacga tcggtgatgc caatcggcgg cactcgctga
    31501 ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt gagtgccaga
    31561 atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg accacttggg
    31621 tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat gccagtttgc
    31681 ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa
    31741 aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat
    31801 tgcccggata gtctatagtc actaggcatt aaaatttgcg cctcataata gttgggccga
    31861 ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt ttccaacggt
    31921 acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg ttatacatca
    31981 aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa gtgaaggata
    32041 agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg caaacagaga
    32101 tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag ccgttgatgt
    32161 ttctgggggt tacgcgacgc tttcttcttt atctttagcg tcaggcagtt ttggcgaaag
    32221 ggcggtggcc ttgctgtcga tccgggtcac cagcagctgg tcgatgcggt agttatcgat
    32281 atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt
    32341 acgcagcata aacatcataa agcctccgat ggtttcgtag ttgcccgact gcgggaactc
    32401 gtcaatatcc agcacgcgca tgacgtcgtc aattggggtg ccgccgtcaa tcagccatga
    32461 gttctcatca cgggcgacaa tctgctcttc cagcccctga ccgaccagat cgcccatcag
    32521 cgtggtcatc acgtcgttga gggtgatgat ccccaccacc agcgcgtact cgttcatgat
    32581 caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa gggttaacgt
    32641 atccggcaca atcagcgtgt tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt
    32701 agccagcacg cggttcagca ggtctttaga atcgacataa ccgatgatgt ggtcaatatc
    32761 ttcattacag acgaggaact tagagtgcgg atgttccgcc accttattct tcaggctttg
    32821 ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg aagacggaac
    32881 ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt tacgtaacac
    32941 gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga tgtcatcttt
    33001 acgtaccatt ggcagtttga aaatacggaa gatcatgttc gccaggccgt tgaagaacca
    33061 caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac gcaaagccac
    33121 cgcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca ggataaacat
    33181 gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagtt ctgccgacat
    33241 atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc
    33301 gagaatcgca actgcgttca gaccgatttg gaccaccgta aagaacatgc cgggattttc
    33361 ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca gtttaagttt
    33421 gattttgcgt gaggcggcaa gtgagatctc agacatcgag aagaacgcac ttacagcgat
    33481 caagcagagt atgactaaaa tactgtttaa catatcttat ccgacctttc aggccggatc
    33541 ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa
    33601 gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt
    33661 aaaattcaga ctgctgataa atgcaatact gcctgatgcg ctatgccgat caggcctacg
    33721 cagtccctgc aatatattga atttggacga ttttgtaggc ctggtaaggc attcacgccg
    33781 cattcggcat aaacaaaacg cactttatca acaatctaca tttgtacccg taacgctatg
    33841 cttccggtgg cagacagacg ccaattccgc caattccaca gtaaccatac gggtttttat
    33901 gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta gcgatttccg
    33961 tggtgatgtg acgatcgtca tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg
    34021 cgcgagctgc ggcatcctgt tctggggtca gcggataaat cgctgaacga tactgcgtgc
    34081 cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa aatacctgta
    34141 gcaactgctc atagctgatg acagaaggat cgtaaacaat gcgtaccgct tcggcatgac
    34201 ccgtatcacc ggagcacact tcccgataag tcggatttgg cgtatagccg ccggtatagc
    34261 ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccaca ccccagaaac
    34321 aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt gagtgaccgt
    34381 tgaccgcatg cagcgtggct acgggcatcg gggtgttacg tccaggcagg gcatcggcgg
    34441 gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga aaatcggtca
    34501 ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt gaataggtct
    34561 aaagtaaaac ataagaaata tttggggttt agtctgcttt ttaatccata ttactggatt
    34621 tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca ggagaaaatg
    34681 tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg
    34741 ccgcgaacgt ccgtctacag gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg
    34801 cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg
    34861 tcgatgatgc catccgcgaa ggtctgaaag cgctgggtta ttaccagccg accattgaat
    34921 ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag
    34981 gcgtgccggt gttaattggc ggcaccgatg tggtattgcg cggcggcgcg cggaccgata
    35041 aagactattt gaaattgctc gatactcgcc cggctattgg cacggtactg aaccagggcg
    35101 attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt tatttcgata
    35161 gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagcc ttctgggata
    35221 ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa
    35281 tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag tacgaatcga
    35341 aagatctggc ggaacttaac cgtcgacttt ctgctaccgg ctggtttaac tcggtcgtag
    35401 tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt acccttgacg ggcgtggttt
    35461 cgccgcgaac tgaaaacacc atcgaaaccg gggtcggtta ctctacggac gtgggaccgc
    35521 gcgtgaaagc tacgtggaaa aagccgtgga tgaactctta tggtcacagt ctgaccacca
    35581 gtaccagtat ttccgcgccg gaacagaccc tcgacttcag ctataaaatg ccgctgctga
    35641 agaatccact ggaacaatat tatttggtgc agggcggttt taagcgcact gacctgaacg
    35701 ataccgaatc tgactccact acgctggtgg cttctcgcta ctgggatctc tccagcggct
    35761 ggcagcgtgc cattaacctg cgctggagtc tcgaccactt tactcagggt gaaattacca
    35821 acaccacgat gctgttttat cctggggtga tgattagccg cacgcgttct cgtggtggcc
    35881 tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac acggcctggg
    35941 gctcagatgt cgatttctcc gttttccagg cgcagaacgt ctggatccgc acactgtacg
    36001 atcgccatcg tttcgttaca cgcggcacgc tgggctggat tgaaaccggt gatttcgaca
    36061 aagtaccgcc ggatctgcgt tttttcgccg ggggcgatcg cagtattcgc ggctacaaat
    36121 acaaatctat cgccccgaaa tacgccaacg gtgacctgaa aggggcctcg aagttgataa
    36181 ccggatcgct ggaataccag tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg
    36241 atagtggcga agcggtaagc gatattcgcc gcagcgactt taaaaccggt accggggtcg
    36301 gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg
    36361 ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta tgagtttatg
    36421 gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat cggtggcgtt
    36481 tctggtgggc accaccagcg gcctgcatct tgtatttaaa gcggcggatc gctgggtgcc
    36541 aggactggat attggcaagg tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg
    36601 ttatgagcag ccaggcgtgg cggtaaaagc gggtaatctg catctggcgg tcggtcttga
    36661 gtgcctgtgg aacagtagcg tttgtattaa tgacctggcg ctgaaagaca ttcaggtcaa
    36721 catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag atagcggtcc
    36781 gctggatctc tccacgccgt atcccatcac cctgacacgg gtggcgctgg acaacgtcaa
    36841 catcaagatt gatgacacca cggtgtcggt gatggacttc acctccggcc tgaactggca
    36901 ggagaaaacc ctgaccctga aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa
    36961 agtggcggaa gtggcgcagg aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga
    37021 ggaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat tgccggaaat
    37081 gaccgacttg catttgccgc ttaacctgaa cattgaagag tttaaaggcg agcagttacg
    37141 cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga gcagcattga
    37201 cggcaatact aaactggacg ccctggatat cgattccagt caagggatcg tcaacgccag
    37261 cggcacggcg cagctgtcag acaactggcc ggtggatatc accctcaaca gcacactgaa
    37321 cgtggagccg ttgaaaggtg aaaaagtgaa gctaaaagtg ggcggcgcgc tgcgcgaaca
    37381 gctggagatt ggcgttaatc tttccggtcc ggtggatatg gatttacgcg cccagacgcg
    37441 actggcggaa gccggattgc cactcaacgt ggaagtgaac agcaaacagc tttactggcc
    37501 gttcaccggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat
    37561 gaccgattac acgctctcta tgcgtacggc agtgaaggga ctggagatcc cgccagccac
    37621 cattaccctt gatgccaaag gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc
    37681 ggcgctggaa gggaaaactg aactcaaggc gttgctcgac tggcagcagg ccattagttg
    37741 gcgcggtgag ctaacgctta acggcattaa caccgccaaa gagatcccag agtggccgtc
    37801 gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct ggcagatgga
    37861 ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg ttgacggcac
    37921 gctgaaaggc aacagttata tgcagtggat gatcccaggg cttcatctgg aactggggcc
    37981 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
    38041 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga aagggctggt
    38101 gaaagttcgc ggcacggtgg aagcgccaca actactggcg gacatcaccg cgcgcggcct
    38161 gcgctggcag gagctaaccg tggcgcaggt tcgcgtggaa ggcgatatca aatccaccgt
    38221 tcagattgcg ggtaaactcg acgtgcgcgt tgagcaaatt tcacagccgg atgtaaatat
    38281 caacctcgtc accctgaatg ccaaaggcag cgaaaaacag cacgagttac agttgcggat
    38341 tcagggcgag ccggtttccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
    38401 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
    38461 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
    38521 ctgctggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg cgggggccga
    38581 agggcgtgcg gtggtgaatc tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc
    38641 agaaaccact caggccagcg gtatcttcac gggtaaagcg gatgttgcct gggacaccac
    38701 gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca
    38761 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagaca ctgaatctga cggcggaatt
    38821 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
    38881 tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
    38941 ccgtaacttc aaccttgcga tgataaaccc catctttaac cgtggggaaa aagcagcggg
    39001 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca
    39061 gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata tgcaaccgag
    39121 ccagcttgcg gtcaacttta atggtatgcg ctcgacgctt gccggtacag tacggaccca
    39181 gcagggtgaa atctacctga acggtgatgc cgactggagc caaattgaaa actggcgggc
    39241 gcgggtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga
    39301 tgtatcgcca gatgttgtct tcgaggctac accaaacctg tttaccctcg atggtcgcgt
    39361 ggatgtcccg tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc
    39421 cagcgatgtg gtgatgctta acgataacct gcaacctgaa gagccgaaaa cggcgtcgat
    39481 tccgattaac agtaacctga ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg
    39541 cctgaaagcg cggctgacgg gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct
    39601 gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg atctgattgt
    39661 gcgtaaaggt gagttactgt tctctggtcc gccggatcaa ccgtatctta atattgaagc
    39721 tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagttcgcg tcactggtct
    39781 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac aagctgcatt
    39841 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg cggcaatgac
    39901 ctcgatgctg attggtctgg gggttgcgca aagtggccag attgtgggta aaatcggcga
    39961 gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact cctcccaggt
    40021 agtggtcagc ggctatgtat tgccaggtct gcaagtgaaa tatggcgtgg gtatatttga
    40081 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
    40141 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
    40201 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
    40261 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
    40321 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgacaa cgccacgctg
    40381 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
    40441 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
    40501 gaaagcggcg actggttaga cagggataag taaccatatg catacgccac cttcgggtgg
    40561 cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcaccc tttgcttttc
    40621 tctccgagcc gctttccata tctgttaacg cataaaaaac tctgctggcg ttcacaaatg
    40681 cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac ttaaggaggt
    40741 gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca ttcccaatat
    40801 cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaagcgcagg tgagcaacaa
    40861 ccaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc tggcacagtg
    40921 tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg
    40981 tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct ggttggcttt
    41041 gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt
    41101 caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg cggaaatttt
    41161 gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt gcacggcgtg
    41221 gtgctggtga atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa gcgtattggt
    41281 ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt ggtggaataa
    41341 ggtgtgttta tttatcgcgg gcataaaaaa acccttacta accgaagctc ggcgttcagg
    41401 gttattgcgc tagaagaact tatttattct ttgcgcgctc gaaggaagca acgatttcag
    41461 ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct ttttcgaggt
    41521 ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggc agatcgttaa
    41581 cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca accagtttcg
    41641 catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa cggatcacag
    41701 aacccggctg cagcgggtac ggagtcggga ccagtacgtc aaccgggtca ccgtccagag
    41761 acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg gacatgaagc
    41821 ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga tctgcgttag
    41881 ccgggatctc aataacaacg tagatgtctt ccggcagatc tttacccgca gggacgttga
    41941 gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag tattatagcc
    42001 aactcgcgcc gaatgtcttt gcttgttttc gtcttcattt tcctttttcg ccagttttaa
    42061 gacggtatac agagcaggaa aatccataac cgtagccgca tttttcatag tgagatgaaa
    42121 gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat gtaacgcaat
    42181 ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca ataataatta
    42241 ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag tctcggcagc
    42301 catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg tcggatcgga
    42361 atccggctgg cgcgctgcag aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat
    42421 tacgttgaaa attgccgatg gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc
    42481 cttcgttgca caaggggtgg atgcgatctt tatcgctcca gtggttgcga cgggttggga
    42541 accggtatta aaagaggcga aagatgccga aatcccggtc ttcttgcttg accgttccat
    42601 cgatgtgaaa gacaaatctc tctatatgac caccgtcact gccgacaaca tcctcgaagg
    42661 caagttgatt ggtgactggc tggtaaaaga agtgaatggc aaaccatgca acgtggtgga
    42721 actgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc
    42781 cattaagaat gcgccaaata tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag
    42841 taaaggcaaa gaagttatgg agagctttat caaagcggaa aacaacggca aaaacatctg
    42901 catggtttac gcccataacg atgacatggt gattggtgca attcaggcaa ttaaagaagc
    42961 gggcctgaaa ccgggcaaag atatcctcac gggttccatt gacggcgtac cggatatcta
    43021 taaagcgatg attgatggcg aagcgaacgc cagcgttgaa ctgacgccga acatggcagg
    43081 ccccgctttt gacgcgctgg agaaatacaa aaaagacggc accatgcctg aaaagctgac
    43141 cctgaccaaa tccacccttt atctgcctga taccgcaaaa gaagagttag agaagaagaa
    43201 aaatatgggg tattgagggt tgctatgcct gatgccgatt cgtaggccgg ataaggcgct
    43261 cgcgccgcat ccggcgatgg tgcactgaag cctgatgcga cgcttaccgc gtcttatcat
    43321 gcctactggg agcacgcttt acaccggggg aaaccatgac gaccgaccaa caccaggaga
    43381 tcctccgcac cgaaggatta agtaaatttt tccccggcgt caaagcgtta gacaacgttg
    43441 atttcagcct gcgccgtggc gaaatcatgg cgctgcttgg tgaaaacggg gcgggaaaat
    43501 caacgctaat caaagcatta actggtgtat accacgccga tcgcggcacc atctggctgg
    43561 aaggccaggc tatctcaccg aaaaataccg cccatgcaca acaactcggt atcggcaccg
    43621 tctatcagga agtcaacctg ctacccaata tgtcggttgc tgataatcta tttataggcc
    43681 gcgaacccaa acgtttcggc cttctacgcc gtaaagagat ggaaaagcgc gccaccgaac
    43741 tgatggcatc ttacggtttc tccctcgacg tgcgcgaacc gcttaaccgt ttttcggtgg
    43801 cgatgcagca aatcgtcgcc atttgccggg ctatcgatct ctccgccaaa gtgctgatcc
    43861 tcgatgaacc caccgccagc ctcgacaccc aggaagtgga gctattattt ggcctgatgc
    43921 gtcagttgcg cgatcgcggc gtcagcctga tctttgtcac tcactttctc gatcaggtct
    43981 atcaggtcag cgatcggatc accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa
    44041 cgcgcgagct accgcagatc gaactggtaa aaatgatgct ggggcgcgag ctggataccc
    44101 acgcgctaca gcgtgccggg cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa
    44161 attacggcaa aaaaggaacg atagcaccgt ttgatctcga agtgcgcccc ggcgagatcg
    44221 tcggtctggc tggcttgctg ggatccggac gtaccgaaac cgccgaagtg atcttcggta
    44281 tcaaacctgc tgacagcggc acggcgttga tcaaaggcaa accgcaaacc ctgcgatcgc
    44341 cacatcaggc ttcagtactg ggcatcggat tctgcccgga agacaggaaa accgatggca
    44401 tcatcgctgc cgcctcggtg cgggaaaata tcatcctcgc tctccaggcc cagcgcggct
    44461 ggctacgacc catttcccgc aaagagcagc aagagattgc cgaacgcttt atccgccagc
    44521 ttggcattcg cacaccttca actgaacaac cgattgaatt tctctctggc ggcaatcagc
    44581 aaaaagtgtt gctttcacgt tggctactga cacgaccgca atttctgatc ctcgacgagc
    44641 caacccgcgg cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt gaaacgctgt
    44701 gtgctgacgg tctggcgctg ttggtgatct cctccgaact ggaagagctg gtgggctatg
    44761 ccgatcgggt gattatcatg cgcgatcgca aacaggtggc ggagatcccg ctggcagagc
    44821 tttccgttcc ggcgatcatg aacgccattg cggcgtaagg agaacagtgt gatgcctcaa
    44881 tctctcccgg acactacgcc gccgaaaagg cgctttcgct ggccaacggg aatgccgcag
    44941 ctggcggcgc tattgctggt gctgctggtc gatagcctgg tagccccgca tttctggcag
    45001 gtggtgcttc aggatgggcg tttgttcggt agccctatcg acattcttaa ccgtgcagct
    45061 cccgttgcgt tactggcgat tggtatgacg ctggtgatcg ccaccggtgg gattgatctc
    45121 tccgtagggg cggtgatggc tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga
    45181 ttcagcctgc cgattgtttt gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg
    45241 aacggcatac tggtagcgat cctcaaaatt cagccgtttg tcgccaccct gatcctgatg
    45301 gtcgccgggc gcggcgtggc gcaactgatc accgccgggc agatcgtcac gtttaactcg
    45361 ccggatctct catggtttgg cagcggatcg ctgttgttcc tgccaacgcc ggtcattatc
    45421 gcggtgctga cgcttctcct gttctggctg ttgacccgca aaacggcgct ggggatgttt
    45481 atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa cacgcggatc
    45541 atcgtcatgc tcacctacgt gttgagcggg ctgtgtgcgg cgattgcggg cattatcgtg
    45601 gcggcggata ttcgcggtgc cgatgccaat aacgccgggt tatggctgga gctggacgcc
    45661 attcttgcgg tggtgattgg cggcggatcg ctgatgggcg ggcgctttaa cctgctgctc
    45721 tcagtcgtgg gggcgctgat tattcagggg atgaacaccg gaattttact ttctggcttt
    45781 ccgccggaga tgaaccaggt cgtaaaagcg gtggtggtgc tttgtgtgct gattgttcag
    45841 tcgcaacgct ttatcagtct gattaaagga gtacgtagcc atgataaaac gtaacttgcc
    45901 gctgatgatc accatcggcg tctttgtgct gggctatctc tactgcctga cccagtttcc
    45961 cggttttgct tccacaagag tgatctgcaa tatcctgacc gataacgcct ttcttgggat
    46021 aatcgccgtt ggcatgacct ttgtgatcct ctctggcggt atcgatctct ccgtcggttc
    46081 ggtgatcgcc tttactggcg tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc
    46141 gctgctggcg tttccgctag tgctggtgat gggctgtgcc tttggcgcat ttatggggct
    46201 tttgatcgac gccctgaaga tcccggcatt tatcattacc ctggcaggaa tgttcttttt
    46261 gcgcggcgtc agctatctcg tttcagaaga gtcgatcccg ataaaccatc ccatttatga
    46321 cacgctctca agccttgcgt ggaaaatccc tggcggcggt cgcttaagtg cgatgggact
    46381 gctgatgctg gcggtggtgg ttatcggcat attcctcgcg catcgtaccc gttttggtaa
    46441 tcaggtatac gccattggcg gcaacgcaac gtcggcaaac ctgatgggga tttctacccg
    46501 cagcaccact attcgcattt atatgctctc caccggactg gcaacgctgg cggggattgt
    46561 cttctcgatt tatacccagg ccggatatgc gctggcgggc gtaggtgtgg aactggatgc
    46621 tatcgcctca gtggtaattg gcggtacgct tttgagcggt ggcgttggaa cggtattagg
    46681 gacgcttttt ggcgtggcga ttcaggggct aattcagact tatatcaact ttgacggtac
    46741 gctgagttcc tggtggacga aaatcgccat cggcattttg ctgttcattt ttattgcctt
    46801 acagcgtgga ttaacggtgc tgtgggagaa tcgtcagagt tcgccagtga caagagtcaa
    46861 tattgcgcag cgataaaaac gcctctccgt gtggagaggc gcaggagatt acgcgtccgg
    46921 gaattcacgg ataaagcgtt cgacatcttc aaccatatgg tcgttgccga caaagaacga
    46981 acggcgctgg tgcagagttt ccgggatgat atccagaata cgctctttgc catcgctcgc
    47041 tttaccgccc gcttgttccg ccaggaatgc catcgggttg cactcataca gcaaacgcag
    47101 tttgccgtcc gggtggctgg cggtgcttgg gtagagataa ataccgcctt tcagcaggtt
    47161 acggtggaaa tccgcgacca gtgaaccgat ataacgtgag gtatacgggc ggttggtgga
    47221 tttatcttct tcctggcaga atttaatgta cttcttcacc ccgttcggaa acttaatgta
    47281 gtttccttcg ttgatggagt aggttttgcc tttctccggg aagcgcatcc gttcctggca
    47341 caggcagaaa acgcccagcg aaggatcgta agtaaaggcg tgaacaccgc atccggtggt
    47401 gtaaaccagc atggtagagg agccgtatac cacgtaacct gccgcaacct gtttgttacc
    47461 aggctggagg aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc ggcggtagat
    47521 ggagaaaatg gtaccgacag agacgttaac atcgatgttg gacgagccat ccagggggtc
    47581 catcagcacc acgtattttg cgtgttcaca gccttcaaag acgacaatct catcttcttc
    47641 ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt gcggctttca gtttttcatt
    47701 agcgaacaag tcgagtttct gctgaacctc gccctgcacg ttctcagcac cgctggcacc
    47761 caggatatca accagtcctg ctttgttgat atcgcgatgg ataatcttgg cgcccagttt
    47821 tattgccgac agcaaagcag tgagctcacc ggtagcatga gaaaactcgt gctgcttttc
    47881 gacaataaat tcacctaacg ttttcataaa actttccctg caatgtttat ggagtaaagc
    47941 gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa
    48001 ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca
    48061 gattaaaaaa ggatagtgac gtatgcgcat tcatatttta ggaatttgtg gcacatttat
    48121 gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa
    48181 tgtgtatccg ccgatgagca ccttacttga gaagcaaggc attgagctga ttcagggtta
    48241 cgatgccagc cagctcgatc cgcagccgga tctggtgatt attggcaacg ccatgacccg
    48301 tggaaatccg tgtgtggaag cggtactgga aaaaaacatc ccttatatgt caggtccaca
    48361 gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg
    48421 caaaactacc accgcgggaa tggcgacctg gattctggaa cagtgcggtt acaaaccggg
    48481 atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga
    48541 cttctttgtt atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac gctctaaatt
    48601 tgtccattac tgcccgcgta cgctgatcct caacaacctt gagttcgatc acgccgatat
    48661 ctttgacgac ctgaaagcga tccagaaaca gttccaccat ctggtgcgta tcgttccggg
    48721 gcagggccgt attatctggc cggaaaatga catcaacctg aaacagacca tggcgatggg
    48781 ctgctggagc gagcaggagc tggtgggtga gcagggtcac tggcaggcga aaaagctgac
    48841 cactgacgct tctgaatggg aagtcttgct ggatggcgaa aaagtgggcg aagtgaaatg
    48901 gtcgctggtg ggcgaacata atatgcacaa tggcctgatg gcgattgcgg cggctcgcca
    48961 tgttggtgta gcgcctgcag atgccgctaa cgcgctgggt tcgtttatta acgctcgtcg
    49021 ccgtctggag ttgcgtggtg aagcgaatgg cgtgacggta tatgacgatt ttgcccatca
    49081 cccgacggca attctggcaa cgcttgcggc gctgcgtggc aaagttggtg gtacggcgcg
    49141 cattattgct gtgctggagc cgcgctcgaa taccatgaaa atggggatct gcaaagacga
    49201 tctggcacct tcattaggtc gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc
    49261 gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct gcacactgga gtggcgatgt
    49321 ggatacgctg gcagatatgg tggtgaaaac cgctcagcct ggcgaccata ttctggtgat
    49381 gagcaacggc ggttttggtg ggatccatca gaaactgctg gatggtctgg cgaagaaggc
    49441 ggaagctgcg cagtaattcg gcctcagcct gatatagtgt gcgtaggccg gataaggcgt
    49501 tcacgccgca tccggcattt gagcatagtg cctgatgcga cgcttgatgc gtcttatcag
    49561 gcctacaggt gcaccgcatc cggcaaacca tactcacatc aacaacgaaa attacccttc
    49621 attctccgcc agttcgcgca gatactggaa aatctggcgt gcggatttcg gcggcttatt
    49681 cccttctttc tctttcttcg cgttgcggat cagagtacgc agctgctgac gatcggcatc
    49741 gtgccacaga tttaatacct cagcgatggc gtcatcacct tgatcgatca gacgatcgcg
    49801 caggttttcc agtttatgga acagcaccac ctgctggttg tgacggtttt tcagcttatc
    49861 cagcgcctga cggatcggtt ctacatcgcg ctggcgcagc atcttaccga tgagctgcaa
    49921 ctggcggcgg cgaccttcca tcttaatacg ctgggcgagc tcaatagccg cgcgcagatc
    49981 cgcatctaac gggatcttat ccagcgcgtt tttccccaga tcaacaattt ccgcgccaag
    50041 gcgttttagc tcctcggcat cacgtttaat ttcactttta ctgacccaga taatttcatc
    50101 gtcttcgtct tcgatgtcat caccgggaac gtcgtcgagc cagtcttcgg gctgcttagt
    50161 catctcaggc tccttaaaaa aagaggctaa tgttaccagt taagatgcgc actgaaaaac
    50221 ggttctctgt tagacttcag agaaactctc tacattatgg cacttgcaat gaaagtaatc
    50281 tctcaagttg aagcgcagcg caagattctg gaagaagcag tttccactgc gctggagttg
    50341 gcctcaggca aatcggacgg tgcggaagtt gccgtcagca agaccaccgg cattagcgta
    50401 agcacgcgtt atggtgaagt ggagaatgtt gaattcaata gcgatggcgc gctggggatc
    50461 actgtttatc accagaaccg caaaggtagc gcatcatcca ccgatttaag cccgcaggcc
    50521 attgcccgta ctgtacaggc ggcgctggat attgcccgtt atacctcgcc agatccctgt
    50581 gccggcgtgg cagacaaaga gctgctggcc tttgacgcac cagatctcga cttgttccac
    50641 cctgcggaag tttccccgga tgaagccatt gaactggcgg cccgcgcaga acaggcggca
    50701 ttgcaggcgg acaaacgcat taccaatacc gaaggtggca gctttaacag ccactacggt
    50761 gtcaaagttt ttggcaacag ccacggcatg ttgcagggtt actgctcaac gcgtcattcg
    50821 ctctccagct gtgtaattgc cgaagaaaat ggcgatatgg agcgtgatta cgcctacacc
    50881 attggccgcg cgatgagcga tctgcaaacg ccagagtggg tcggggccga ctgtgctcgc
    50941 cgtactttat cgcgcctgtc accgcgtaaa ctctccacca tgaaagcgcc agtcattttt
    51001 gccaatgaag tggcaaccgg gctttttggc catctggtgg gggcgatagc gggtggctcg
    51061 gtttaccgta aatctacctt tctgctggat tcgctgggta aacaaattct gccggactgg
    51121 ctgaccattg aagagcatcc gcatctgctg aaagggctgg cgtcgacgcc attcgacagc
    51181 gaaggtgtgc gcaccgagcg tcgcgatatt attaaagatg gcatcctgac tcagtggctg
    51241 ctgaccagct actcggcgcg gaaactgggg ctgaaaagca ccggacatgc gggcggtatt
    51301 cacaactggc ggattgccgg acaagggcta agcttcgaac aaatgctcaa agagatgggc
    51361 accgggctgg tggtgacgga gctgatgggc cagggcgtga gtgccattac cggtgattat
    51421 tcccgtggtg cagcgggctt ctgggtagag aatggcgaaa ttcagtatcc cgttagtgaa
    51481 atcaccatcg caggtaattt aaaagatatg tggcgcaata ttgtcaccgt cggtaacgat
    51541 attgaaacac gcagtaatat acagtgtggt tctgtgctgt tgccggagat gaaaatcgcc
    51601 gggcagtaaa aaactggcgc gacctgccgc gctataaact aaaaaattac acaaaatcat
    51661 tcgcactgca tcgagacgga aagtgaatga gggccgggga gcgtactcgc agtacgtgac
    51721 ccgggcgaat gagcgtagcc gtcgatgagg cagtgtgaag gatgaagtgt aaataaaaaa
    51781 ggaagtgagc aatgcgtaaa agcctgttag ctattcttgc agtctcctcg ttggtattca
    51841 gttctgcgtc gtttgccgct gatcttgaag a
//
LOCUS       DAZFDP010000016        48861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFDP010000016 DAZFDP010000000
VERSION     DAZFDP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            400..1617
                     /gene="alaA"
                     /locus_tag="ACHJ1G_001051"
     CDS             400..1617
                     /gene="alaA"
                     /locus_tag="ACHJ1G_001051"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4762944.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1701..2300
                     /gene="yfbR"
                     /locus_tag="ACHJ1G_001052"
     CDS             1701..2300
                     /gene="yfbR"
                     /locus_tag="ACHJ1G_001052"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4762945.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2359..4191)
                     /locus_tag="ACHJ1G_001053"
     CDS             complement(2359..4191)
                     /locus_tag="ACHJ1G_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4762946.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4278..4928)
                     /gene="hxpA"
                     /locus_tag="ACHJ1G_001054"
     CDS             complement(4278..4928)
                     /gene="hxpA"
                     /locus_tag="ACHJ1G_001054"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4762947.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4939..5433)
                     /locus_tag="ACHJ1G_001055"
     CDS             complement(4939..5433)
                     /locus_tag="ACHJ1G_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4762948.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5516..5971)
                     /gene="yfbV"
                     /locus_tag="ACHJ1G_001056"
     CDS             complement(5516..5971)
                     /gene="yfbV"
                     /locus_tag="ACHJ1G_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4762949.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6309..7511
                     /gene="ackA"
                     /locus_tag="ACHJ1G_001057"
     CDS             6309..7511
                     /gene="ackA"
                     /locus_tag="ACHJ1G_001057"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4762950.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7586..9730
                     /gene="pta"
                     /locus_tag="ACHJ1G_001058"
     CDS             7586..9730
                     /gene="pta"
                     /locus_tag="ACHJ1G_001058"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4762951.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9920..11440
                     /gene="yfcC"
                     /locus_tag="ACHJ1G_001059"
     CDS             9920..11440
                     /gene="yfcC"
                     /locus_tag="ACHJ1G_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4762952.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11473..12015)
                     /gene="yfcD"
                     /locus_tag="ACHJ1G_001060"
     CDS             complement(11473..12015)
                     /gene="yfcD"
                     /locus_tag="ACHJ1G_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4762953.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12073..12624)
                     /gene="yfcE"
                     /locus_tag="ACHJ1G_001061"
     CDS             complement(12073..12624)
                     /gene="yfcE"
                     /locus_tag="ACHJ1G_001061"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4762954.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSLPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12680..13324)
                     /gene="yfcF"
                     /locus_tag="ACHJ1G_001062"
     CDS             complement(12680..13324)
                     /gene="yfcF"
                     /locus_tag="ACHJ1G_001062"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4762955.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13460..14107
                     /gene="yfcG"
                     /locus_tag="ACHJ1G_001063"
     CDS             13460..14107
                     /gene="yfcG"
                     /locus_tag="ACHJ1G_001063"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4762956.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14164..14526
                     /gene="folX"
                     /locus_tag="ACHJ1G_001064"
     CDS             14164..14526
                     /gene="folX"
                     /locus_tag="ACHJ1G_001064"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4762957.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14547..15440
                     /locus_tag="ACHJ1G_001065"
     CDS             14547..15440
                     /locus_tag="ACHJ1G_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4762958.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15488..16378)
                     /gene="rpnB"
                     /locus_tag="ACHJ1G_001066"
     CDS             complement(15488..16378)
                     /gene="rpnB"
                     /locus_tag="ACHJ1G_001066"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4762959.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16575..17348)
                     /gene="hisP"
                     /locus_tag="ACHJ1G_001067"
     CDS             complement(16575..17348)
                     /gene="hisP"
                     /locus_tag="ACHJ1G_001067"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4762960.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17356..18072)
                     /locus_tag="ACHJ1G_001068"
     CDS             complement(17356..18072)
                     /locus_tag="ACHJ1G_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4762961.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18069..18755)
                     /gene="hisQ"
                     /locus_tag="ACHJ1G_001069"
     CDS             complement(18069..18755)
                     /gene="hisQ"
                     /locus_tag="ACHJ1G_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4762962.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18845..19627)
                     /gene="hisJ"
                     /locus_tag="ACHJ1G_001070"
     CDS             complement(18845..19627)
                     /gene="hisJ"
                     /locus_tag="ACHJ1G_001070"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4762963.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19848..20630)
                     /gene="argT"
                     /locus_tag="ACHJ1G_001071"
     CDS             complement(19848..20630)
                     /gene="argT"
                     /locus_tag="ACHJ1G_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4762964.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20896..21465)
                     /gene="ubiX"
                     /locus_tag="ACHJ1G_001072"
     CDS             complement(20896..21465)
                     /gene="ubiX"
                     /locus_tag="ACHJ1G_001072"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4762965.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21560..23077)
                     /gene="purF"
                     /locus_tag="ACHJ1G_001073"
     CDS             complement(21560..23077)
                     /gene="purF"
                     /locus_tag="ACHJ1G_001073"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4762966.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23114..23602)
                     /gene="cvpA"
                     /locus_tag="ACHJ1G_001074"
     CDS             complement(23114..23602)
                     /gene="cvpA"
                     /locus_tag="ACHJ1G_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4762967.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23861..24523)
                     /gene="dedD"
                     /locus_tag="ACHJ1G_001075"
     CDS             complement(23861..24523)
                     /gene="dedD"
                     /locus_tag="ACHJ1G_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4762968.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24513..25781)
                     /gene="folC"
                     /locus_tag="ACHJ1G_001076"
     CDS             complement(24513..25781)
                     /gene="folC"
                     /locus_tag="ACHJ1G_001076"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4762969.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25851..26765)
                     /gene="accD"
                     /locus_tag="ACHJ1G_001077"
     CDS             complement(25851..26765)
                     /gene="accD"
                     /locus_tag="ACHJ1G_001077"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4762970.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26921..27580)
                     /locus_tag="ACHJ1G_001078"
     CDS             complement(26921..27580)
                     /locus_tag="ACHJ1G_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4762971.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27663..28475)
                     /gene="truA"
                     /locus_tag="ACHJ1G_001079"
     CDS             complement(27663..28475)
                     /gene="truA"
                     /locus_tag="ACHJ1G_001079"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4762972.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28475..29488)
                     /locus_tag="ACHJ1G_001080"
     CDS             complement(28475..29488)
                     /locus_tag="ACHJ1G_001080"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4762973.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29554..30690)
                     /gene="pdxB"
                     /locus_tag="ACHJ1G_001081"
     CDS             complement(29554..30690)
                     /gene="pdxB"
                     /locus_tag="ACHJ1G_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4762974.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30789..31784
                     /gene="flk"
                     /locus_tag="ACHJ1G_001082"
     CDS             30789..31784
                     /gene="flk"
                     /locus_tag="ACHJ1G_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4762975.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31781..32959)
                     /locus_tag="ACHJ1G_001083"
     CDS             complement(31781..32959)
                     /locus_tag="ACHJ1G_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4762976.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33243..34463)
                     /gene="fabB"
                     /locus_tag="ACHJ1G_001084"
     CDS             complement(33243..34463)
                     /gene="fabB"
                     /locus_tag="ACHJ1G_001084"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4762977.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34622..36628
                     /gene="mnmC"
                     /locus_tag="ACHJ1G_001085"
     CDS             34622..36628
                     /gene="mnmC"
                     /locus_tag="ACHJ1G_001085"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4762978.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36749..37027)
                     /locus_tag="ACHJ1G_001086"
     CDS             complement(36749..37027)
                     /locus_tag="ACHJ1G_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4762979.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37061..37609)
                     /gene="epmC"
                     /locus_tag="ACHJ1G_001087"
     CDS             complement(37061..37609)
                     /gene="epmC"
                     /locus_tag="ACHJ1G_001087"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4762980.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37609..38418)
                     /locus_tag="ACHJ1G_001088"
     CDS             complement(37609..38418)
                     /locus_tag="ACHJ1G_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4762981.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38418..39242)
                     /gene="mepA"
                     /locus_tag="ACHJ1G_001089"
     CDS             complement(38418..39242)
                     /gene="mepA"
                     /locus_tag="ACHJ1G_001089"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4762982.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39246..40331)
                     /gene="aroC"
                     /locus_tag="ACHJ1G_001090"
     CDS             complement(39246..40331)
                     /gene="aroC"
                     /locus_tag="ACHJ1G_001090"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4762983.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40366..41298)
                     /gene="prmB"
                     /locus_tag="ACHJ1G_001091"
     CDS             complement(40366..41298)
                     /gene="prmB"
                     /locus_tag="ACHJ1G_001091"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4762984.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41464..42015
                     /gene="smrB"
                     /locus_tag="ACHJ1G_001092"
     CDS             41464..42015
                     /gene="smrB"
                     /locus_tag="ACHJ1G_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4762985.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42088..42939)
                     /locus_tag="ACHJ1G_001093"
     CDS             complement(42088..42939)
                     /locus_tag="ACHJ1G_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4762986.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42941..43480)
                     /locus_tag="ACHJ1G_001094"
     CDS             complement(42941..43480)
                     /locus_tag="ACHJ1G_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4762987.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43477..43965)
                     /locus_tag="ACHJ1G_001095"
     CDS             complement(43477..43965)
                     /locus_tag="ACHJ1G_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4762988.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43962..44471)
                     /locus_tag="ACHJ1G_001096"
     CDS             complement(43962..44471)
                     /locus_tag="ACHJ1G_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4762989.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44487..45239)
                     /locus_tag="ACHJ1G_001097"
     CDS             complement(44487..45239)
                     /locus_tag="ACHJ1G_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4762990.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45259..47904)
                     /gene="yfcU"
                     /locus_tag="ACHJ1G_001098"
     CDS             complement(45259..47904)
                     /gene="yfcU"
                     /locus_tag="ACHJ1G_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO4762991.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47986..48549)
                     /locus_tag="ACHJ1G_001099"
     CDS             complement(47986..48549)
                     /locus_tag="ACHJ1G_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4762992.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11977 a        13212 c        12181 g        11491 t
ORIGIN      
        1 atcctcattc attaattccg cttaacaata gtttcacacc ttgcccccat tacgacacct
       61 cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc gcgtttcatt
      121 agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa aaccagactt
      181 acaattaaga atcagaacaa ttcactatat aacattgcat gtaaagcata tacacctcat
      241 tattttgtca ttattaagtt attaacagca caatcaagcc ttcccctctg gcaaaatctt
      301 attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac
      361 ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc
      421 agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc
      481 ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccgttcggt
      541 tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg
      601 tattgcgatt ccaaaggtct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct
      661 cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt
      721 atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca
      781 gattacccac tatggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt
      841 tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct
      901 cgtacgcgtg ggatcgttat tatcaaccca aataacccaa ccggcgcggt atattccaaa
      961 gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat
     1021 gaaatttatg acaaaattct ttacgacgac gctgagcatc actcaattgc gccgctggca
     1081 cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc
     1141 cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt
     1201 ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag
     1261 accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat
     1321 gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa
     1381 cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcacgac
     1441 gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg
     1501 gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat
     1561 atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct
     1621 taatttcact gccggagatt gcatccggca gcgttatccc gccacaatga cctgatgatg
     1681 tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga
     1741 aactcattaa ccgctggccg ctcatgcgca acgtgcggac ggaaaatgtg tccgaacaca
     1801 gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgg aaatttggcg
     1861 gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc
     1921 tcaccggcga tctccctact ccggtgaaat acttcaattc gcaaatcgct caggagtaca
     1981 aggctattga aaaaatcgct cagcaaaaac tggtcgatat ggttccggaa gagctgcggg
     2041 atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga
     2101 aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata
     2161 atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg
     2221 agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta
     2281 gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcaggcaat aagcgccgga
     2341 tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc
     2401 catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc
     2461 cggacctaaa accagtgtgt taacaggtga agaaaccggt gtcataaagg cggcggatgc
     2521 tgccatcgcc acgaccatcg cgaatggata aggcgacacc cccatcgttt tggcagcagc
     2581 cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaata gcccaataac
     2641 cgccgacaag acaaacaaac accccagcat catatgtggc ccgtaaccac cgccaatgtc
     2701 catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg
     2761 catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc
     2821 atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg
     2881 aatttcatct gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc
     2941 ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc
     3001 gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc
     3061 gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat tgcgctttag
     3121 ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat
     3181 cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga
     3241 aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag
     3301 atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac
     3361 cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt
     3421 agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgatcc
     3481 ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg
     3541 aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa
     3601 acgcatcact aacatataca aaatacccag caccagcacg accaggccaa taggtgttac
     3661 gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt
     3721 cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat
     3781 catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa
     3841 gatagcgaca acgccggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa
     3901 caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg ttcccattac
     3961 ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac
     4021 aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc
     4081 aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa
     4141 gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc
     4201 ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta
     4261 aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta
     4321 atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga
     4381 gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca
     4441 tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac
     4501 gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg
     4561 ggaagcccag ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg
     4621 gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc cggaagcgcg
     4681 gtaatacctt cggtttccgt ggcctcgatg tgctccagac gcgtaaactc ggcggcaata
     4741 tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga
     4801 atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc
     4861 cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg
     4921 caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg cgctcaaatg
     4981 gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg
     5041 cgtctgggcg ttaaaaccgt gagttccggc gtcaaaatgg gtatagcgcc cttccacatt
     5101 aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa
     5161 ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg aaacatgcaa
     5221 cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc
     5281 aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag
     5341 gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga
     5401 caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctatactacg
     5461 gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca
     5521 aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg
     5581 cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac
     5641 cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc
     5701 ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagcgcagtg gcaactgccg
     5761 gcccaagctg accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca
     5821 taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggggcaa
     5881 ggcgtttttc cagcggccac gtctttgagt aatgctgtcc ccggcgaaac aagctaaaaa
     5941 aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt
     6001 caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat
     6061 acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc
     6121 gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca tagactcaag
     6181 atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt
     6241 gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag
     6301 gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat
     6361 ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc
     6421 acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag
     6481 gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa
     6541 aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa
     6601 agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt
     6661 cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt
     6721 tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc
     6781 cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc
     6841 gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga
     6901 acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt
     6961 ctgctatccg caacggtaaa tgcgttgaca cctctatggg cctgaccccg ctggaaggtc
     7021 tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca
     7081 ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg
     7141 gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag
     7201 acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca
     7261 ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac tggtggtatc ggtgaaaatg
     7321 ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc
     7381 atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc
     7441 gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc
     7501 tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat
     7561 cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac
     7621 cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt
     7681 tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca
     7741 gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat
     7801 gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt
     7861 cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc
     7921 gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc
     7981 ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat
     8041 cgaactgacc cgcaacagct tcggcggtgc caaaaacacc aacatcaccg gcgttatcgt
     8101 caacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccagatc tgtccgagat
     8161 tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc
     8221 cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc
     8281 gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg
     8341 ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg
     8401 tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct
     8461 ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga
     8521 cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt
     8581 gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt
     8641 tgacgatcac gagcgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga
     8701 ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgcctgcgtt
     8761 ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa cgtatcgtac tgccggaagg
     8821 tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg
     8881 cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact
     8941 gggtgcaggg attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct
     9001 ggtcgaactg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga
     9061 caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg
     9121 tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc
     9181 gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta
     9241 cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca
     9301 gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc
     9361 caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc
     9421 gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat
     9481 ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt
     9541 catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga
     9601 cctgatctcc atcgggccga tgctgcaggg tatgcgcaag ccggttaacg acctgtcccg
     9661 tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca
     9721 gcagcagtaa tctcgtcatc atccgcagct ttgcgctgcg gatatctgaa ccggaaataa
     9781 tcactatttc cggtttttta ttctcttaat ctgcattaat cctttctgat tatcttgctt
     9841 aactgcgctg catcaatgaa ttgcgccatt tcactttgca tacttaccac tttgttttgt
     9901 gcaagggaat atttgcgcta tgtccgcaac cactgaatcc aaaccaacaa gaagatgggc
     9961 aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagcc ttgccacctg
    10021 ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac
    10081 aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg aagcaggcga
    10141 acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa
    10201 cttcccgttt gaaggattaa cctcaggatc gaaatacggg acagctgttg gcatcatcat
    10261 gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa
    10321 cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct
    10381 gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt
    10441 tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct
    10501 ggtgacctat attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt
    10561 ggtcgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt
    10621 ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg cctcacgagt
    10681 gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca
    10741 ggctgatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac
    10801 cgccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat
    10861 tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct
    10921 taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat
    10981 cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggtcg gtaatggtga
    11041 agcaggtgat gccagcgtgt taaataccat cctcaacagc attgccaatg ccattagcgg
    11101 tctggacaac gctgtcgcgg cctggtttat gttgctcttc caggcggtat ttaatttctt
    11161 tgtgacatcc ggttctggtc aggcggcgtt aaccatgccg ttactggcac cgcttggcga
    11221 tctggtcggt gttaaccgtc aggttaccgt gctggcattc cagtttggtg atggcttcag
    11281 ccacatcatt tacccaacct cagcttcgtt aatggcgacg ctcggtgttt gccgggtgga
    11341 cttccgtaac tggctgaagg tgggcgcgac actgcttgga ctgctgttta ttatgtccag
    11401 cgtcgtggtg atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt
    11461 gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt
    11521 catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat
    11581 ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtaggg cgaagggacc
    11641 gtgagagacg cagctgaaca atgcgcccca gacacggcaa tttttatctt cgaaatagaa
    11701 ctgcccgtgc tcagcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg
    11761 cgcggattcc agcagttgct catcggcctg gaccacaccg cctgcggtcg catctaacat
    11821 gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc
    11881 atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct
    11941 ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa
    12001 acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga
    12061 gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc
    12121 gattacacta agtacgtcat tatccagcat gccataactc gccggattac cgcctttcgg
    12181 aagactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag
    12241 atgggtatgg ccgtacacca gcacatcgtt ctggtttaaa gcaggcagat tttccgggcc
    12301 aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg
    12361 cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt tgccgcgcac
    12421 agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc
    12481 ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg
    12541 ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg acccatgaat
    12601 gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata
    12661 ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga
    12721 cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc
    12781 catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac
    12841 caaataaatt cggctgaccc agtactaaca aatgttctgc catcgcaaac agtttctctg
    12901 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    12961 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    13021 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    13081 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    13141 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    13201 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg
    13261 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    13321 tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact
    13381 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    13441 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    13501 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    13561 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    13621 aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg agtctggtgc
    13681 cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg
    13741 cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca
    13801 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    13861 ggttgaaact cagcgtcttt accatgtact gaacaagcgg ctggaaaact cgccctggct
    13921 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    13981 tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    14041 ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga
    14101 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    14161 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc
    14221 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    14281 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    14341 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta
    14401 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    14461 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    14521 cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    14581 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg gtagtgacgc
    14641 gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg caagggctag
    14701 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    14761 ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    14821 cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta ctcatttccg
    14881 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    14941 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    15001 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    15061 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg
    15121 gtcggcagta tctggcctgg attcatattg atgatatggt caacggcatt ctctggctgc
    15181 tggataacga cctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac
    15241 aatttgccca tgcgctcggt caagcgctgc atcgcccggc cattttgcgc gtccccgcaa
    15301 ccgccattcg gctgttaatg ggcgaatctt caatactggt attaggtgga caacgcgcgc
    15361 tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc
    15421 tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctcccggtgt
    15481 tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa
    15541 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt
    15601 gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct
    15661 tttcttgtgg tgcgcgttct gttatctcac caataaatgc acggaaacgc tgggcatccc
    15721 ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa
    15781 ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct
    15841 gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg
    15901 taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa
    15961 attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca
    16021 tcgggatcac caatggaagc tctttatagc ccgcatcaag atggttttgc attgccgcaa
    16081 tggaataacg catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca
    16141 ctacataaat ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat
    16201 attgccgcag gtcgtcatca ataaaactgt ttggttccag tttaagcgtc gttaaatcac
    16261 acagtttgcg cagcggcgcg ggaagatgaa tatcaataaa atcccgcgcg gtgtctggat
    16321 ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg
    16381 cgttccgtca cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc
    16441 agcaggcagt ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt
    16501 ttacatttca gcggaagctg actcacaaat tccccatgaa aatactccgg cagcgttagc
    16561 caccggagag gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc
    16621 gggttgccaa ataactgctc cggcgcgccc tcttcttcta tttttccctg atggaggaaa
    16681 atgacgtgag tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt
    16741 ttcccctctt ctgccagttg ctgcataata cgtaacactt cgcctaccag ttcaggatcg
    16801 agcgccgagg taggttcatc aaacagtaaa acttccggtt ccatcgccag cgcccgcgcg
    16861 atagaaacac gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt
    16921 tcgtctatcc cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc
    16981 aggcccaaca cctgaatcgg cgcttccatg acgttttcca gcaccgtcat atggctccag
    17041 agattgaagt gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta
    17101 tcggcgactt tgagttgacc atctttgtcg cgcaccagat tgatcgtctg gccattgacc
    17161 acgatcgacc cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc
    17221 cccgatcccg acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc
    17281 cctttcagca cttcatgttc gccgtagcgt ttgtgcaaat cgataacgtt taatttattc
    17341 tcggacatcg tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc
    17401 cgctctgcga aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat
    17461 gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag
    17521 atccggcaca gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt
    17581 gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcaac gatacatttt
    17641 aaaagtcgag aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat
    17701 tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt
    17761 acagttcagg ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag
    17821 cgtgtacatg ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat
    17881 ataggtaaat aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc
    17941 cagaaacagc gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat
    18001 cgccacacca gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa
    18061 gatttcgatc acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc
    18121 agcacaccat tggaaacagt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa
    18181 tagaacggtt cccaggtact tttgcctgcc agttgggtgg ctttgaccac atcttccagg
    18241 ccgagtaacg aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct
    18301 ggcagggcgt aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccacgagta
    18361 aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca ctgccataaa cgcgccacgg
    18421 aacgtttcgg taaaataagc accgtagata aaaccgagag taataatacc agcgaccatc
    18481 ggatcgatat caatctgccc gacgcccatc gcctccgtca ccgtgtttag tgcaatctgc
    18541 aaaccgtaga aaatcagcag catcaacacc aagtccggca cgcctcgaat cagcgtggtg
    18601 tacccttcga aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt
    18661 aaaccgatga ttacagcgag cactacagaa ctgatagcca gctccagcgt gacgagcgca
    18721 ccctgtaaaa taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg
    18781 aattaccgcc tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg
    18841 gcgattaacc gccataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac
    18901 cgtcagcgcg catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt
    18961 tacgcaggcc catgccggtc cctacgccaa acagtttttc atctttaaca gacgggccac
    19021 cgaatttgta atctttaccg acaggttgtt tgaggaaacc ttcgctggca gcgacctcat
    19081 cctggaacgc ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct
    19141 gatacgagac gatttcaatg ccttttggtg cccaatgttc attaccgaac gtctcctggg
    19201 tggtgccctg caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt
    19261 cggaattttt cgccaccacc aaacgagaat cggcagcgta cagtttgtcg gtgaaggcta
    19321 tttcttgctg acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg
    19381 cttttaagga cgggattaac gcatccagcg gattttcgac aaacgtacat tgcgtattga
    19441 tgcgtttgca taattccttt gccagatcga tatcgaagcc aaccagttcg ccttgtgaat
    19501 tttttgattc aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaatcg
    19561 cagcaaacgc cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt
    19621 ttttcataat gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac
    19681 ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc gccccgttag acgtattatt
    19741 tttcattaaa aatgcttaag tatgcatttc aatgcaccac gatagtgcaa caaacctggc
    19801 gtgcaccatg atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag
    19861 acattaaagt cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg
    19921 ccaagcgcct tattgaaggc agccgtcagt tcagcatcat ctttacgtag ccctacaccg
    19981 gtgccatcac cgaagtattt tttgtctttt actgatgagc cagcaaaggc gaaatcttta
    20041 ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc
    20101 agacgtcctg cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc
    20161 acgcctttgc tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca
    20221 ccaacatgtt tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca
    20281 atcaaacgag aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta
    20341 tcggtaatgg aaagcgacga aataatggcg tcgatttttt tcgctttcag tgaggggatc
    20401 agcgcgtcaa agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg
    20461 ttaccgagat cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacggc
    20521 gcataggtgg tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc
    20581 gctgcggaga gaccgactaa caaagacaga gcgagaatcg acttcttcat acataaccct
    20641 caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg
    20701 caggtcttat gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac
    20761 gttgcattat tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg
    20821 attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac
    20881 agggcaacag cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat
    20941 tgcaaactgg tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg
    21001 gcgatgataa aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt
    21061 cattaaacgc agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg
    21121 acgctctttc agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac
    21181 aatgccggaa agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga
    21241 accggaagag atgctggcgg aaatatcgcg cgcatcgtgc gtgacatcag ctaatgcctg
    21301 cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat
    21361 caccagatgc gtttcgatat ctgtgacatc gcgcagaacc tgtaataagc gcacgccata
    21421 aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt
    21481 cagacttgca ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc
    21541 ggcaccctca gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct
    21601 gacgttgcac tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagccct
    21661 gatcaacatc tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat
    21721 ccggattttc agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt
    21781 cagcaccgat gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg
    21841 cgctcggcat atcaatacca taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga
    21901 ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc
    21961 cacggacgat ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg
    22021 cattcagttt acggcgcacg gacttacgac gcagctgctg gcccggcatg ataaaggtgc
    22081 ggccaacata gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgagcaa
    22141 tttccagcgc gatatcacac gaggtttctg ggatcgggat caccacgtcg atatccagat
    22201 cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt
    22261 aaacggaaat tttgtcgatg aacgagtccg ggcgagcaaa gtatacatac tcaaacaggc
    22321 acggattgct gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga
    22381 tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg
    22441 ctacgctttc ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca
    22501 gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca
    22561 tcgccacaca ggcatatgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa
    22621 tattgtcggc ttccagcggg tagtggcgga agttgtccag ctcgctagcg aagatattaa
    22681 gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt
    22741 tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacggag
    22801 agttaacgta aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca
    22861 catgaccaat gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc
    22921 tcaccagccc gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc
    22981 cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa
    23041 ccggcataac accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca
    23101 catctcagag cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa
    23161 accatctgat gataaaactg aattgcggga tcagctgtga tttgctccag tcttcgcttt
    23221 tcgacacccc ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc
    23281 gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca
    23341 ccaactggcc tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta
    23401 ccgcgatggc aattccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca
    23461 ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg
    23521 cttcacgaac aaagccgcgg atcaggctaa ccagagagga aaaagcaatc accgcgatta
    23581 tggcgtaatc aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg
    23641 ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata
    23701 aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc
    23761 aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccagg
    23821 cctgataaga cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca
    23881 ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat
    23941 ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg
    24001 gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat
    24061 cggcattttt cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt
    24121 cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc ttcggtttag
    24181 gcggctccac cggtttcgac ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg
    24241 tgttattggc tgcaatagtg gtcggatcga gcgacggtgc tgcggcatca cctgcccgca
    24301 cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca
    24361 tatcaggctc atcgcgatcg cccgctttcg gcaccagcgg gatagccgcg aactcatcct
    24421 gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca
    24481 gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca
    24541 atcacttcca tgacatgtgc gacagtgtgg aaagaaccac acaccagcac ggtgtcttcc
    24601 gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg
    24661 ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca
    24721 caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca
    24781 tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg
    24841 gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg
    24901 ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt
    24961 tcactgactt ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc
    25021 gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca
    25081 tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc
    25141 tgtgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg
    25201 ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc gatctggacc cagccagtcg
    25261 gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg
    25321 tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgcttgaac
    25381 agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc
    25441 gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg gccctgcaca
    25501 cgtacgcgct cggtataacg caccagatgc ggtgaactgt agacgcccac tttgtaccct
    25561 gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca
    25621 acggtaaaca caaatggcgc tggtttcaga acgccaagac gcgccgcgac ctggctcacg
    25681 cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa
    25741 gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta
    25801 cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca
    25861 ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc
    25921 gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga
    25981 cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatcctggc
    26041 ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct
    26101 ttcggttcag cgatgttgag atcgcccagc atggcgaaac ttgcagaaac accgcccatc
    26161 gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc
    26221 gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca
    26281 gaggcggaga agcagatcag cgggcagtta tcttccagcg cctgctcaac ggcacgcacg
    26341 aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca
    26401 gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgcca
    26461 gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc
    26521 acatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta
    26581 tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga
    26641 ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc
    26701 ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag
    26761 ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca
    26821 taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga
    26881 aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg
    26941 cagcgcgttt gtgacgaata aattcgaaaa ccccaggcaa tattgaaaca aaaataatgc
    27001 caacaatcat cagcttaagg ttgctctgaa tgaaggggag cgtgccgaag aaataacccg
    27061 cgtaggtaaa aatcagtacc cacaacagtg cgccgatcac gttataagcg gcgaaatgac
    27121 ggtacgacat gtggcccatt cccgcaacaa acggcgcgaa cgttctgacg atcggtacaa
    27181 aacgggcgag aataatcgtt ttgccgccat gtctctcgta aaactgatga gtcttatcaa
    27241 gataactgcg acgaaagatt ttcgaatccg ggttactgaa cagtttgtca ccaaataacc
    27301 gtccaatggt atagttcact gcatcaccga caatagcggc aatcaacata agcaccacca
    27361 tcatatggac attgagatcg ttagtttcca gcgacgccaa tgcaccagca acaaacagca
    27421 gcgaatcacc aggcaggaat ggcgttacca ccagccccgt ttcgcagaac agaatcaaaa
    27481 acagaatggc ataaacccag acgccgtact ccgcgaccag ctctgccaga tgcacgtcaa
    27541 tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact
    27601 cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct
    27661 cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg
    27721 taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc ccgcgttctg
    27781 tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc
    27841 atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatct
    27901 accaccacat aaggaccgtg acgcatgaca ttaatatgca taacgttgcg ccacggggtt
    27961 cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc
    28021 gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc
    28081 agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg
    28141 ctaaatcggg catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca
    28201 ggtaaattcg catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg
    28261 aaatgcacca cttgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg
    28321 gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg
    28381 acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca
    28441 attttataaa ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt
    28501 tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc
    28561 agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg
    28621 gcgtacccga agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat
    28681 cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa
    28741 agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca
    28801 tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca
    28861 cgctaccttc gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga
    28921 aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat
    28981 cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca
    29041 aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg
    29101 gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca
    29161 ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac
    29221 ctgagttggt cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg
    29281 ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac
    29341 caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg
    29401 ggaactgacg ttcagccagc gtttcaagca gggcttcgcc cacagcgcca gttgcgccca
    29461 ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct
    29521 gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt
    29581 aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga
    29641 tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc
    29701 ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct
    29761 tttcagcgtc ggttgatcga gcgggccatg cagcgtaatg cgaccaaact ctggcgcggg
    29821 cagtaatgta tccagcgcaa cgtgctgttc atgcccaata aacttgctat aggcttcaaa
    29881 cacttgcgta gtaccgcgtg ctttaccttc cagggtataa cctgcgatat gcggcgtgcc
    29941 gatatccact tttgtcagca actccacgtt aagttccggt tcgccttccc agacatccag
    30001 cactacgctt aacttctggc cttcattcag gcaggtcagc aacgcagtat tatcgacgac
    30061 tgcgccacgg caggcgttaa tcagaatcgc tcccggcttc aggctacgga tcagtttttc
    30121 atccgccaga tgcagcgttt tgtacggccc atctttgaag agtggcgtat ggaaagtcag
    30181 aatatccgca tgctgaacta attcatccag cgagcggaaa tcaccctcat ccccacggtc
    30241 agcgcgaggc ggatcgcaaa gcaatgtttt aatccctaac gcttccagtc gcgcctgcaa
    30301 tcgacgtcca acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc
    30361 atcgcgttcg gcaagcatca gtagtgagga gaaaacatat tccaccaccg caatcgcatt
    30421 acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg
    30481 gtcggtcccc gcagtggcag tgccaacaaa tttaatgggt tttcctgcca gcaaagattc
    30541 attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcaacggg
    30601 gattggacgc ccgggaaccg cggtcacctc acccaaacgg ctaaataagt cgcgggcata
    30661 aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta
    30721 accggacaag tgtgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag
    30781 gatacgtaat gatacaacct atttccggcc ctcctcctgg gcaaccacca ggtcagggag
    30841 ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg
    30901 aaagcttaat gacgaaagtg acctcactga cgcaacaaca aagagcagaa ctgtgggcgg
    30961 gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg
    31021 ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc
    31081 agcttttagc gcaattaggg gagtatttac gtctggggaa taatcgtcag gcggtcacgg
    31141 attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa
    31201 aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca cagcagcgcg
    31261 aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca
    31321 aacttgcggc ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt
    31381 ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggt
    31441 tgcaggcgcg ccagacgtta agccagcaaa atacgccgac gctggaatca ctacagatgg
    31501 cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa
    31561 aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc
    31621 agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc
    31681 cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac
    31741 tgttagtcgc gattatcctg atgctggtct ggctggttcg ttagccccga cgaaatgaca
    31801 gtatcgtgac aataattccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt
    31861 aaccaaacgt ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct
    31921 gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca
    31981 cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg
    32041 ctaatgcgac ccacgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa
    32101 gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga
    32161 cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc
    32221 ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta
    32281 gtgccagccc taaccctggt ttccagataa acccgacaac gctccacagc gatggacgtt
    32341 ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta
    32401 ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa
    32461 gcggagcacc aacagcgagg gcaccgtaaa tcgccattcc attccatgac atcactttgc
    32521 cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc
    32581 tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg
    32641 agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc
    32701 cctgaagcgc cgaacgtttt gcaccatatt gatcggccag tcgcccggca tagccacgcg
    32761 tcagcaccgt agccagaaac tgaatcccga cggcaatccc gaccatggta ttgccatagc
    32821 ccagatcatg atgaacaaac agcgggataa ccggcaacgg cagccctacg gtcatgtagg
    32881 tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt ggcagaagat cgtgtttcgg
    32941 tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg
    33001 tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg tacgtctttt
    33061 cagaaggaaa tcgacaaagc gggaagtttg cctggaacag gcggcgattg tcaatgatgt
    33121 gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat tagcgcgtaa
    33181 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc gactactccg gcctactgcg
    33241 aattaatctt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa gccgaagctg
    33301 ttagacataa cggtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt cagacctgca
    33361 gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg ttccagcatc
    33421 agcagagagt agatagcttc ctgtacgcca gcagcgccca gagagtgacc ggtcatggct
    33481 ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc tgccagttct
    33541 ttcacgtcgc caaccggagt cgaagtacca tgagagttca ggtaatcgat tggggtatca
    33601 acgccgtgca tcgccatctt catgcagcgt actgcgcctt cgccagacgg agcaaccatg
    33661 tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgtg agcaccacgc
    33721 gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc gataacgaaa
    33781 ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt gtatttagta
    33841 gacagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc ttcgccgccg
    33901 ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt accgatacag
    33961 tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat tttaaacggc
    34021 gtggcgaggc aggcagaaac gccggatgcc atcgctttgg tgaccacata cgggccaacc
    34081 gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa acgcggggaa
    34141 ccgccgccag aacctgcaat caggccaacg cgcgggttat tctggtaagc ttccggagaa
    34201 aggcccgcat ctgcgattgc ctgctccata gaaaggaatg cataaatgga tgcgtcgctc
    34261 ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttac gttgccccag
    34321 acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc tgaacgtcct
    34381 tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac aatgcccagg
    34441 ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga gtaagtttcg
    34501 aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt aatagagaaa
    34561 tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac gagtaactta
    34621 cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg gtacacctgt
    34681 ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag agacgcgtta
    34741 tgtttttctg ggaggcaacc aattagaggt acgctttcct gagcatccac atcctctgtt
    34801 tgtggtagca gagagcggct tcggcaccgg attaaacttc ctgacgctat ggcaggcatt
    34861 taatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt tcattagttt
    34921 tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact ggccggaact
    34981 ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg gttgccatcg
    35041 tttattgctc gatgaaggcc gcgtgacgct ggatttatgg tttggcgata ttaacgaact
    35101 gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc tggacggctt
    35161 tgcgccagcg aaaaacccgg atatgtggac gcaaaatctg tttaacgcca tggcaaggtt
    35221 ggcgcgtccg ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc gccgcggttt
    35281 gcaggacgcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac gggaaatgct
    35341 ttgcggggtg atggaacaga cattaccgct cccctgctcc gcgccgtggt ttaaccgcac
    35401 gggcagcagc aaacgggaag cggcgattat cggcggtggt attgccagcg cgttgttgtc
    35461 gctggcgcta ttacggcgcg gctggcaggt aacgctttat tgcgcggatg aggcccccgc
    35521 actgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca aacacgatga
    35581 ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt acgaccaatt
    35641 acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg atgagaaaag
    35701 ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg ctgtagccgt
    35761 tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg gcattactta
    35821 tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg aactggcgca
    35881 acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc gtaaggatga
    35941 ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag tggtactggc
    36001 gaacgggcat caaatcagcc gattcagcca aacgtcgact ctcccggtgt attcggttgc
    36061 cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc aggtgctgtg
    36121 ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta ttggtgccag
    36181 ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc agaatcgcca
    36241 gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg tcagtgataa
    36301 agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg taggcaatgt
    36361 tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaga aagatgaggc
    36421 ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt ctcgcggttt
    36481 gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg aaccgattcc
    36541 gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc ggaaattgtt
    36601 gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg gatgcggcgt
    36661 gaacgcctta tccgacctac gtgtgacgtg taggcctgat aagacgcgat aagcgtcgca
    36721 tcaggcatgg tgctccagac gccgcaactt actgctgtga cgcctgctga aacaggtttt
    36781 cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca ccaatggctt
    36841 tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca tcaccacttt
    36901 ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca aacaactcat
    36961 catcactggc atggtcaacc ataccgtcga ttaatgccag aatgcgtgat tcaaactccg
    37021 cgatcatctt ctttcctcat tgtaaaacgt ggcaccggct tcagttgagc gcttccggcc
    37081 acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt gctttaatga
    37141 agcgtgccgg gcgttcagga atgccgtttg ctaggtaatc catcacctgc gcatgtaccc
    37201 gccgttgaaa caccacacga tccggttcga agtcgccttc cagattgtcg caactgacgt
    37261 taaacggata tcccgccgcc acacagaaca accagtcgaa cgcctgcggc ttcacttcaa
    37321 catcttcaaa ctggctttgc gtttgggcat cgcgtccgtc cgggcaatac cagtagccga
    37381 aatcaaccag ctcacggcgc gctttcccag caatacacca gtgcgaaatc tcatgaattg
    37441 cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc gcatcagcag
    37501 gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca tcggcaaagc
    37561 agctattaaa aatttcaatc aactgctcat agtggtgtgt actgttcatt agttcatccc
    37621 caaccagtgg aggatctcct gtccgtggct atcaaaaagt aatttggcac tcatcaccgc
    37681 tgagacaata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca ccagtcgtga
    37741 acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg cccaaatcac
    37801 tttgccaccg agaataaaca gcagtaaacc gccgatgttt gacgttgcgt tgagtaattt
    37861 ggcgtgcgcc gtggctttgg cgaggttgaa tccgcacagc gtaacaaagg ccagtgcgta
    37921 aaacgatccg gctgccggac caaagaatcc gtcataaaaa ccgacgcagc caccggcaat
    37981 taacgcgaag ggtaaaccgt acatccgacg ctggcgatct tcttcaccca gctttggcat
    38041 cagcaagaaa tagaggccga tacaaattac cagaatgggc aaaatctgcc gtaagacatc
    38101 agcctgaacg tattgcacca gcagtgcgcc gctcattgag ccgacaaagg tcatggcgat
    38161 attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga tagtagcgga
    38221 aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcattagcgg gagacatccc
    38281 cgctgccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg agtcgataaa
    38341 tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggggaaa ccataaacag
    38401 gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca ggaaggcggc
    38461 aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg ttcaaaccag
    38521 ctttgcagtt ctgccccgca accatcgcct ggtggcggta aaggttgatc ttcacactcc
    38581 agactatcgg caggacaacg taatcgtaca tgcatatgcg cacgatgctg gaaccagggt
    38641 cgcactttgc gcaaccagtc gcgatcggtg cccgcatcta ggcaaagttg ttgtttaatc
    38701 gccggattaa caaaaatgcg cgtgacgttt ttgtcctggg cggcgagttt gatcaagctg
    38761 aaaatttctg gcttccacag cgaggagaca acgtgtttac cgtcgcggga aactaagtcc
    38821 agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag ttgcagaaag
    38881 atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg cccaccagca
    38941 ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac ctggctactc
    39001 agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg atcggtacgc
    39061 atgacctgat aatgttcgga ctgtatcggc agcgtgtcag cgccgacaat acagccatta
    39121 gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt ttgccacggc
    39181 gtcgctgcca ggctagcgct actggcaagc agagccagca gcgcaatcgc ggttttgttc
    39241 attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg ctgccgtaac
    39301 aggtgatcca ttaaaacgat cgccagcatc gcttctgcga tcggcactgc acggatcccg
    39361 acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa gcggttaata
    39421 gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgggc aatgatttgc
    39481 tgcccgctgc tgataccgcc gagaatgccg cccgcatggt tgctctggaa accgtctttg
    39541 gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc gtcgccaatt
    39601 tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc atccaggcgg
    39661 tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt gactttcgcg
    39721 ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc gtcgattttg
    39781 tccgggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt gatttccagc
    39841 ggaatgtcgc ccatctgggt caagcagccg cggatttcaa taccaaattt ctcggcgaga
    39901 tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc ggaagaacgt
    39961 ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc agcatggcct
    40021 gggcggaaaa cgtctttaat tgcgctgtaa tcctgagagc gctggtcagt gttttcgatc
    40081 aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag aattttgacc
    40141 tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg acggtcgagg
    40201 tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc gacgatgcag
    40261 ccgagcgcca gcccatgcga ttcgccgaag gtggttacgc gaaagagttg tccaattgtg
    40321 tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat ctttataaat
    40381 cgcgaaatat tcacgggccg ccagcagctg ctctttggtg agcataaaca caccatcgcc
    40441 gccgttatca aactccagcc aggtgaacgg aacatccgga tattgttcca taagatgtac
    40501 catgctgttg ccgacttcac aaatcagcac accatcatca gcaaggtaat ctgccgcgtt
    40561 accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc ccagttccgg
    40621 ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat acggcgggtt
    40681 agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg aacgaatcgg
    40741 aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg ccagcgcgtc
    40801 gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac aggcaatggc
    40861 gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc tgatgagtcc
    40921 ggcaaattta ttgttgatca gttcaccaat cggtgagcgc ggcaccagca cgcgttcatc
    40981 gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca ccggaatgcg
    41041 ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg aggtcagacg
    41101 cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta acaccagctg
    41161 tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg ccgcgctgaa
    41221 gcggcttacc gaccagcgca acatgtcctg aatggtttgc agctcattta ctgcttcatc
    41281 aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc ggcggctagt
    41341 ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa aaatacgttt
    41401 aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa tagagacaga
    41461 taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg ccagttgatg
    41521 gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg taaaaaaatc
    41581 agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca ttatttctcc
    41641 gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg cccggatgtc
    41701 agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt gtttttggat
    41761 ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat tgccgcctgt
    41821 cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca tattttgaag
    41881 caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca ccaggcaccg
    41941 aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga gtggttgccg
    42001 ccggagttgc cctgaggagt tgagcgtgtt gtgccaggag cgcggaatgg caggggcgaa
    42061 tgaccctgcc aggtgagaaa aggtgcgcta atacatattc agtatgtaag tgaatcggca
    42121 ggtagaatct atgggctgtc gagatgtcgt gtccacgcgt agcttcatat catgcagttg
    42181 cctggacgtg atcggttgcg gctctggcgt caccgtcact ttggcattat cacagccgga
    42241 agtcgagaag gtcatctgct gtgcgacaaa acggcagtag gctccacgct cgtcaaaatt
    42301 actgccatca cacagtttgc cgttttccat caggaaagtg ggcagtgttt gctcaataac
    42361 accacctgtt gactgcaacc tgacgacttt gttatgtaca acataatttt cattccagga
    42421 aacatcatac ggtgccaggg actctgtcgg gcagtcagag ctaacttttg ttggtcctat
    42481 aaaaattccg gccccgaaac gctgatccat agtactggcg ctttcaacat aagacgacac
    42541 aatccaggga caaccgtgta aatctccatc gtaataaacg ccaagtacta attcattaca
    42601 tgaccaggcc gttactggag agagagcatc atactcttct aatccggggc aggtagaatc
    42661 cggtaaggtt gtattattgc gctccatcct gagttcaggg ccgggaggac tcccggtcca
    42721 actaactaaa ggcaaacttg ctgccgcagt tgaattagat tcatacgtgc catattcaac
    42781 tgattgaggg gtcaggactg aaagatgaaa acgatacttt gcaacaacgc tggtcgtccc
    42841 cgcgggtaca tagagtgtgg cgatctttgt cgcagcctgt atcccagaag cgcacagagc
    42901 cagcaccaat aaacatataa catgacgtat tttatgcatt ttatatatac tcaaacgtag
    42961 ctgtcaggct ggcggtgaaa tcaccgatgg ttacatcacg accgcttttc gcctggatcc
    43021 aggcccggaa attaatatca ttgttgccgt tggtaagcgc aaaagtcgcc ccggtggtgt
    43081 tgttaatatc cacccgcttg ccgtcgagcg tttccatccc tatccccacg ccctgcgcgg
    43141 tggaggtgtc gtcgactgcc agaaaacccg gctgaccgct gtcttccgtt ccggtgaggg
    43201 tgactttgac gttgtatccc gccggccctt tgcagtcttt cagcttaaac acaaccggca
    43261 gatgcgcgga ctgcccggcg accatcaaat cccggttgct gacggtggga aaatgaattt
    43321 ccgccagatt ggcaccatcc accaccagcg tgcaggattt actcagcagg ttgccgtaaa
    43381 aatgcaggtt gttatcgact gcccgtgccc ccgtgctggc ggtcagcagc aggcctgcta
    43441 acagcgcata accgggtgaa taaatgtgtg ctgttttcat tggtaatcca cccgtaaggt
    43501 tgcgtaagca gaaaaattcg tgtccgtcag caccgcatca gccgctttga ccggtaccgc
    43561 ctccagcttt ggcttctggc taaaatcggt ggcgttaatg gaaagcggct cgttgagctt
    43621 aaaacgctga ccgtcatgct gaagctcaat tccgaagccc ggtacatccg tctcaatagc
    43681 agagtcgttg taggaggtgg cagtccccgt ccaggagagt gtcatattcc actcgggatc
    43741 gcggacatcc gagtcgcagc tcagttcgta gtccactttc tgacgaccat aattgccatc
    43801 aatgctgtcg ataataagat cgcgaaattg cacttcgatg gtttgcccgc cgctgatgct
    43861 acaggcgggc ggcgacatca gcgtaccgtg gaatgtgatg tcatcttccg ccgccagcac
    43921 agccccgcaa ccagcgcaca gtaaaaggag caggaatttt ttcattggta atccaccacc
    43981 atggtcatcg tggcgttaaa ctccgacggc gtcagttgca ccccgctctg ttttaccggc
    44041 accgctttca gctgaggctg agtgttgaca ttaaaaggcg tccagttatt ttcaccaact
    44101 gcaaacaggg agttgtcggc ggcgttttct atccgcaccc ccaggccggc gatatccgtg
    44161 ctgagaaccg tttctccgtt gatcacggcg gtattgcctt tgagctgcat ccgtaagtca
    44221 tcgctgactt tattaccgca gttgagggtg tatttcgcat cctgttggta attggtacca
    44281 tcgatcctgt tgattatcac attgccaaat ttcacagcgc ttcccttcac ggagcaaggc
    44341 ggcggtccat cgaccaggat tcgcagcgtc agatccacct gtttgatatc actgccagca
    44401 acagccaaac cgctgatgcc ccatccggcc agcaccagca accctgcgcc cgctacaaac
    44461 ttgtgactca taagtccctc ccggcatcag cctttgcggc tctgctcttc atccacctgg
    44521 cagctgttgc cggtgcatat gaagtacagc ggcatccgcg cgccgtagtc gttgatgtaa
    44581 gtcagcaccg gcacgctgtc catcttcgca ttcagcggtg cggaggtttt cggctccatc
    44641 atcagcggtg tgaatcccgc cggcgggttg ccgccttttt tagcgctggc gttgctgatg
    44701 atcacgtagt acggcgtcgg gttattgacg gtgaagtcct ggccgttacg gatgagcgtc
    44761 accttatgct gccacggact tttcatgctg gtgttctcca gcgccttcgg tcgccagaac
    44821 agtttgatac gcgtctgcag cgcaatctgc agtgtgttcg gtttatttga tttcggcgga
    44881 atttcgcgca cgttgaaata gaacacgctc tcgcggtcgg ccggcagttt gttgatatcc
    44941 ggcagcccct gcaccttcac ctggccgttc atcatcgcat cgatacgctg cactggcgga
    45001 agcaccgcaa gcggcgagct gattttgttg ccctttgcat cctctatcca gctctgcgcc
    45061 agataaggca gtgtgggatc gttgttgcgc agcgtgacgc tgatggactt gtcgctttcg
    45121 ttaaacacca gccgtgtcct gtcgggcgtc accgaagcgt ggctggagaa actgaccact
    45181 gtcgccagca gcgccagtgc cgggacaacg atttttgcag tgttagtcag attaaacatg
    45241 gatcattcct taatcacgtt actggtttac tgaaattgaa gtcggaggtt ccgttggcgt
    45301 cacccgctgt gtctgttcct gaatcgccgg tttgaccgcc ggcgaagtga taggtgctac
    45361 attgccttta tgctggcatg gcagtaacaa gccgttaaac agatcggcag gcagcgggtc
    45421 cggcaggttg atatcgcaat gcgcaacacc actccagaac acactcatgt gttcgccagg
    45481 tttcacccca gccagataaa cattgccgtc atcatcgaca aggcccactg tctgctcgtt
    45541 atcatttttt acttctgcgc caaacggagg atggctgccg tcgctcaggc gcagcaccgc
    45601 catcgctttt tgtccgctaa tgacagcaaa tttacggtag ccaatggcac cttcagttag
    45661 cgtggcttgc accaccgact gggttgcttc agcgttttca ggcaatctgt tgaggtcgat
    45721 atacgcctga ttgcggtaat agttattgac gtcagaaacg acggctttac caaacatatt
    45781 ggtataaaca gcagccccgt taccttccac cggaacatcg gcaacgccat cggcatcaat
    45841 caacaatcgt gtcccgccca tattctgggt acggtgaagt gcgccaccgt gggcagtaag
    45901 cgttgcgccg ccctgtaacg acaagcccgc agaggtgtat tgcccttcat ggtagttcgc
    45961 actgaggtca acctgcgcca gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg
    46021 tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg tcatcgacac ggctgaaata
    46081 accgacctga ctgctgtccg tcccactgcc atagttaccg ttatagctaa cggtactgtt
    46141 gtcgccccac ggcatactga gcgaaatgta catgcctttg tcggcctggt tgtcatactc
    46201 gtaacggtag ccagtcatcg agatgctgac attacgaata ctgcccatat tgaaatagtg
    46261 cgagagcatg atgttgtagt ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata
    46321 gttgagataa accgaaacac ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat
    46381 ctctttgtcg ttgcccgtgc gtaccattcc gctgtcactg gcatccagat actcgctcat
    46441 ggtcataaag ttctcttccg agaagcgata tccagcaaaa gtaacgcggc tgttgagctg
    46501 gtcaaagtct ttggaataac tcacacggaa ggagttaccg tccagcgaac ctttgccata
    46561 agcggtgtct ttatccagtt tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa
    46621 tgtagacaaa tcgcgaccga cgccaagcgc cgcagactga tagttttcat ctcccagtgc
    46681 gccgccatat aacgaccagc cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa
    46741 ttcaccctcg atatggtgtc cccactcttg cggacggccc atcatgatct tatagcgaac
    46801 ctgacctggg cgagtgaggt atggcatcga ggcggtgctg atgtcatatt cctgcacctg
    46861 gccgttctgt tcttcaatgc gaatatgcaa cgtaccggag acagaatcac caagatcctg
    46921 aatacgaaac ggtccagccg gaacctgcgt ttcgtaaatc acacgcccca tctggctgac
    46981 ggtcactttt gctgtggtgt gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt
    47041 gggaggcaac atttgatcgt cagtactgac actgccacca acatagttaa agccatcaaa
    47101 aatatcggat ctgaggtaat cctcgcccag cgccagtttg gcttttaatg atggtaacgc
    47161 ccgccaggca tagtagcgac tccactccca ttttttttga gtttcatcgc cgctgaattc
    47221 atcgtcatca tcattactgc gagtatgttg atagttagtc tgccagtcag cacgcatacg
    47281 ccacggcccc aggttaaccc cgaccgtccc gttgccgctg atctcgttac tatcatcacc
    47341 gccattttct tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga tcccggagat
    47401 gccgtcatcc caacgtgaag gcggatccca gtcgggataa gtatattcga ggtaagcctg
    47461 tggcagtgaa atgactaatg cggactggct taaatcagcc ttaatttcca tgccttccag
    47521 ttgaccggat ttcaggcatt tagcatcgtg gctccattgc agatttttcg ccacgtcttc
    47581 ttttaaacca aactgcgcta ccagttccgg tgtcagacaa gcatagcttt tgctcgcgtc
    47641 atcttcacca gcataccagt aaatatcgta ctcttccgcc aatggctgtt tatttagttg
    47701 aacctgtaaa ttgtattttc cgggctcaac atatccctgg ctggaaaaac gcttcagatc
    47761 aatttttgtg tcgcctttta attccagaaa acgggaatca aactgaatgt cgtcttcagc
    47821 ccagacactg ctataactcc ctgacattgc cagcgcaatg caccacggaa gaatccgtaa
    47881 tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt ttttattctt gttcggtcaa
    47941 aatccattta aatcccccat agcagtgtcg ctatgggggt atgccttaca ggtaagtaat
    48001 ctggaaggtg gtatttgctt cgaaattacc cagatctggc gcatcagctg cgccgaccag
    48061 ccaggcttta aagttcagtg tctgcttcgc tttgccttta ttggtagaag tgtctttcac
    48121 tattttctgg tcaataccca tgccgctttt gtaagaggta cccagagagt caccgatcgc
    48181 caggctaaca ttgttaaatg ccgcaccggt atcggtattg aaaatggtgt aataattgcc
    48241 gctatttgca gaagaaacgg taccagtaaa ggtagtggtc attgtttcct gagtatcaaa
    48301 tacgcaatct tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt tatttttcag
    48361 tttttcggca ccgatatcac ccatatcaac ttccagtttg tgatcgtccg gaacgataga
    48421 acacactgag tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac ccgcagcgat
    48481 tgttgccgta gaggtgaaaa cgcccatcac cattgccgca gcaatagctg ttttaacaaa
    48541 cttactcatt atttgtcctt aaactataaa taattaaaaa tataaacatt acaatacatt
    48601 gattcagtca atacccaatg tttataccca gccattgaat acggcatgcg atccaaaaag
    48661 aacaggtcgt agaaaattgt attctttggt gtggggccgc atagtgatgt tttcaccccc
    48721 ctgatgcaat ttaattttac taaagagtaa atgtagattt aattaatata ttgacagaag
    48781 aaaggttatc ttaaggacat ccttaaaaaa tacggaataa gtatttagaa aaaatagaaa
    48841 gtatatcaat aatgaatatc a
//
LOCUS       DAZFDP010000017        48092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFDP010000017 DAZFDP010000000
VERSION     DAZFDP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(111..374)
                     /gene="cedA"
                     /locus_tag="ACHJ1G_001100"
     CDS             complement(111..374)
                     /gene="cedA"
                     /locus_tag="ACHJ1G_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4762993.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            557..2818
                     /gene="katE"
                     /locus_tag="ACHJ1G_001101"
     CDS             557..2818
                     /gene="katE"
                     /locus_tag="ACHJ1G_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4762994.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3077..3835)
                     /gene="chbG"
                     /locus_tag="ACHJ1G_001102"
     CDS             complement(3077..3835)
                     /gene="chbG"
                     /locus_tag="ACHJ1G_001102"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4762995.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3848..5200)
                     /gene="celF"
                     /locus_tag="ACHJ1G_001103"
                     /gene_synonym="chbF"
     CDS             complement(3848..5200)
                     /gene="celF"
                     /locus_tag="ACHJ1G_001103"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4762996.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5305..6147)
                     /gene="chbR"
                     /locus_tag="ACHJ1G_001104"
     CDS             complement(5305..6147)
                     /gene="chbR"
                     /locus_tag="ACHJ1G_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4762997.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6155..6505)
                     /gene="chbA"
                     /locus_tag="ACHJ1G_001105"
     CDS             complement(6155..6505)
                     /gene="chbA"
                     /locus_tag="ACHJ1G_001105"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4762998.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6556..7914)
                     /gene="chbC"
                     /locus_tag="ACHJ1G_001106"
     CDS             complement(6556..7914)
                     /gene="chbC"
                     /locus_tag="ACHJ1G_001106"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4762999.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7999..8319)
                     /gene="chbB"
                     /locus_tag="ACHJ1G_001107"
     CDS             complement(7999..8319)
                     /gene="chbB"
                     /locus_tag="ACHJ1G_001107"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4763000.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8618..8956)
                     /gene="osmE"
                     /locus_tag="ACHJ1G_001108"
     CDS             complement(8618..8956)
                     /gene="osmE"
                     /locus_tag="ACHJ1G_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4763001.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9158..9985
                     /gene="nadE"
                     /locus_tag="ACHJ1G_001109"
     CDS             9158..9985
                     /gene="nadE"
                     /locus_tag="ACHJ1G_001109"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4763002.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10215..11102
                     /gene="cho"
                     /locus_tag="ACHJ1G_001110"
     CDS             10215..11102
                     /gene="cho"
                     /locus_tag="ACHJ1G_001110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4763003.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11062..11637)
                     /gene="ves"
                     /locus_tag="ACHJ1G_001111"
     CDS             complement(11062..11637)
                     /gene="ves"
                     /locus_tag="ACHJ1G_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4763004.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11845..12330)
                     /gene="spy"
                     /locus_tag="ACHJ1G_001112"
     CDS             complement(11845..12330)
                     /gene="spy"
                     /locus_tag="ACHJ1G_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4763005.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12660..13628)
                     /gene="astE"
                     /locus_tag="ACHJ1G_001113"
     CDS             complement(12660..13628)
                     /gene="astE"
                     /locus_tag="ACHJ1G_001113"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4763006.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13621..14964)
                     /gene="astB"
                     /locus_tag="ACHJ1G_001114"
     CDS             complement(13621..14964)
                     /gene="astB"
                     /locus_tag="ACHJ1G_001114"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4763007.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14961..16439)
                     /gene="astD"
                     /locus_tag="ACHJ1G_001115"
     CDS             complement(14961..16439)
                     /gene="astD"
                     /locus_tag="ACHJ1G_001115"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4763008.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16436..17470)
                     /gene="astA"
                     /locus_tag="ACHJ1G_001116"
     CDS             complement(16436..17470)
                     /gene="astA"
                     /locus_tag="ACHJ1G_001116"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4763009.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17467..18687)
                     /gene="astC"
                     /locus_tag="ACHJ1G_001117"
     CDS             complement(17467..18687)
                     /gene="astC"
                     /locus_tag="ACHJ1G_001117"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4763010.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19133..19939
                     /gene="xthA"
                     /locus_tag="ACHJ1G_001118"
     CDS             19133..19939
                     /gene="xthA"
                     /locus_tag="ACHJ1G_001118"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4763011.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20106..20816
                     /locus_tag="ACHJ1G_001119"
     CDS             20106..20816
                     /locus_tag="ACHJ1G_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4763012.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20821..21498
                     /gene="ydjY"
                     /locus_tag="ACHJ1G_001120"
     CDS             20821..21498
                     /gene="ydjY"
                     /locus_tag="ACHJ1G_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4763013.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21513..22220
                     /locus_tag="ACHJ1G_001121"
     CDS             21513..22220
                     /locus_tag="ACHJ1G_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4763014.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22220..22768
                     /locus_tag="ACHJ1G_001122"
     CDS             22220..22768
                     /locus_tag="ACHJ1G_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4763015.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22778..23944
                     /locus_tag="ACHJ1G_001123"
     CDS             22778..23944
                     /locus_tag="ACHJ1G_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4763016.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPEKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23917..25452
                     /locus_tag="ACHJ1G_001124"
     CDS             23917..25452
                     /locus_tag="ACHJ1G_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
                     /protein_id="HFO4763017.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRTKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25452..26105
                     /locus_tag="ACHJ1G_001125"
     CDS             25452..26105
                     /locus_tag="ACHJ1G_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4763018.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26172..27479
                     /gene="ynjE"
                     /locus_tag="ACHJ1G_001126"
     CDS             26172..27479
                     /gene="ynjE"
                     /locus_tag="ACHJ1G_001126"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4763019.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27488..28108)
                     /locus_tag="ACHJ1G_001127"
     CDS             complement(27488..28108)
                     /locus_tag="ACHJ1G_001127"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4763020.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28195..28602
                     /gene="nudG"
                     /locus_tag="ACHJ1G_001128"
     CDS             28195..28602
                     /gene="nudG"
                     /locus_tag="ACHJ1G_001128"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4763021.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28568..28840)
                     /locus_tag="ACHJ1G_001129"
     CDS             complement(28568..28840)
                     /locus_tag="ACHJ1G_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4763022.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29076..30419
                     /gene="gdhA"
                     /locus_tag="ACHJ1G_001130"
     CDS             29076..30419
                     /gene="gdhA"
                     /locus_tag="ACHJ1G_001130"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4763023.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30536..31576)
                     /locus_tag="ACHJ1G_001131"
     CDS             complement(30536..31576)
                     /locus_tag="ACHJ1G_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4763024.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31704..33665)
                     /gene="topB"
                     /locus_tag="ACHJ1G_001132"
     CDS             complement(31704..33665)
                     /gene="topB"
                     /locus_tag="ACHJ1G_001132"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4763025.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33670..34713)
                     /gene="selD"
                     /locus_tag="ACHJ1G_001133"
     CDS             complement(33670..34713)
                     /gene="selD"
                     /locus_tag="ACHJ1G_001133"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4763026.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(34830..35381)
                     /locus_tag="ACHJ1G_001134"
     CDS             complement(34830..35381)
                     /locus_tag="ACHJ1G_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4763027.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35542..37398
                     /gene="sppA"
                     /locus_tag="ACHJ1G_001135"
     CDS             35542..37398
                     /gene="sppA"
                     /locus_tag="ACHJ1G_001135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4763028.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37565..38581
                     /gene="ansA"
                     /locus_tag="ACHJ1G_001136"
     CDS             37565..38581
                     /gene="ansA"
                     /locus_tag="ACHJ1G_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4763029.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38592..39233
                     /gene="pncA"
                     /locus_tag="ACHJ1G_001137"
     CDS             38592..39233
                     /gene="pncA"
                     /locus_tag="ACHJ1G_001137"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4763030.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39394..39666)
                     /locus_tag="ACHJ1G_001138"
     CDS             complement(39394..39666)
                     /locus_tag="ACHJ1G_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4763031.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39708..40121)
                     /gene="msrB"
                     /locus_tag="ACHJ1G_001139"
     CDS             complement(39708..40121)
                     /gene="msrB"
                     /locus_tag="ACHJ1G_001139"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4763032.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40463..41458
                     /gene="gapA"
                     /locus_tag="ACHJ1G_001140"
     CDS             40463..41458
                     /gene="gapA"
                     /locus_tag="ACHJ1G_001140"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4763033.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41542..42426
                     /locus_tag="ACHJ1G_001141"
     CDS             41542..42426
                     /locus_tag="ACHJ1G_001141"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4763034.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42477..43331)
                     /gene="yeaE"
                     /locus_tag="ACHJ1G_001142"
     CDS             complement(42477..43331)
                     /gene="yeaE"
                     /locus_tag="ACHJ1G_001142"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4763035.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43421..44167)
                     /gene="mipA"
                     /locus_tag="ACHJ1G_001143"
     CDS             complement(43421..44167)
                     /gene="mipA"
                     /locus_tag="ACHJ1G_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4763036.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44603..46537
                     /gene="yeaG"
                     /locus_tag="ACHJ1G_001144"
     CDS             44603..46537
                     /gene="yeaG"
                     /locus_tag="ACHJ1G_001144"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4763037.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46650..47933
                     /locus_tag="ACHJ1G_001145"
     CDS             46650..47933
                     /locus_tag="ACHJ1G_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4763038.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        11862 a        12591 c        11857 g        11782 t
ORIGIN      
        1 atagatcgtc aaaggcagct ttttgttact ggtggtttga atgaatgtag caatgaaata
       61 cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg tacgtaatct ttactcagtc
      121 acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg cacagtaaac
      181 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      241 atccagacat cacgaaaaga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      301 cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt cattactggc
      361 ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta gcccctgtac
      421 gtcccacttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcgggatctg
      481 gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc agtaataaat
      541 taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc accagtcacc
      601 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aggacggatc
      661 tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc ctgggagcct
      721 gaaagcccct gatacacgta acgaaaaact taattctctg gaagacgtac gcaaaggcag
      781 tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact
      841 gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      901 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat cagcagctca
      961 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1021 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gcgctggctc
     1081 tgccgatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg aagagggtat
     1141 ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1201 cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag ggcaaagtgc
     1261 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1321 ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct tcggtattca
     1381 caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc
     1441 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg gacgtgaccc
     1501 ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc cggaatacga
     1561 actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg atcttctcga
     1621 tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1681 caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1741 tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt tgttctccta
     1801 taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc cgattaaccg
     1861 tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg ggatcgacac
     1921 taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg aaacaccgcc
     1981 gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa ataaagttcg
     2041 tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2101 gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtcgt
     2161 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2221 ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga atatcacccc
     2281 acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2341 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2401 ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac tgctctactc
     2461 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2521 cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata tcgcggatat
     2581 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2641 tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2701 agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac tgctaacgct
     2761 gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc ctgcctgatg
     2821 ggaggcggcg caattgcgcc gcctcaaatg atttacatag tgcgctttgt ttatgccgga
     2881 tgcggcgtga acgccttatc cggcctacaa agtcgtataa attcaatgta ttgcagttta
     2941 ttggtagata ctgataagcg aagcgcatca ggcagttttg cgtttgtcag cagtctcaag
     3001 cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga tgcaaggttc acgccgcatc
     3061 tggcaaacat cctcacttac acatcccgat aactccccaa ccgataacca cgctgagcga
     3121 tagcaccttt caacgacgct gatgtcaaca catccagctc cgttaagcgt gggaaacagt
     3181 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaac
     3241 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
     3301 caccataaaa cgcgctactg aatccctgag aactgcgcag attagccggt aaatcaccag
     3361 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
     3421 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
     3481 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
     3541 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
     3601 cgcgggttaa ccccggcata gctgtcaggg gcttgcccat agtaaggaca aagtgcatcc
     3661 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
     3721 tcaccagcgc cgtcgttgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
     3781 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
     3841 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
     3901 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
     3961 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
     4021 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
     4081 gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cttacaggtc atttctaccg
     4141 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
     4201 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
     4261 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cggattttta tacagctcaa
     4321 aaagttgttt ctcgactttc tgtactacct gtgctcgtgc gccgcctttg tagtattcgc
     4381 ccatttcaat agccagcatc tctttctgct tgaagtaata cagcagataa gaacatggca
     4441 gcagatttaa cgaacgaatt aagccctcac taaatggcag atcgaaaata ttttttacag
     4501 aggacgcttt taactgccct gacgccacac catcaagcaa ttcggcaaag cgcgacttgc
     4561 catttaccag cacatcctta atgaacacca tatggttgag gccgaacaga tcgatagata
     4621 aatcatcgct gtctttcagc atcagaacat cgcgaataaa catcttcatg ccgatcggaa
     4681 tattacacac gccgataaag cgtttaaatc cggtatgacg ataaacggct tcagtgacca
     4741 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
     4801 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacagaccg cccgcgccgt
     4861 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcgcgcgccg
     4921 gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
     4981 gatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
     5041 gttcaaaaat gatatccagt ttctctttac catcttcgac atccaccagc cataattcgc
     5101 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
     5161 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
     5221 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
     5281 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct
     5341 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
     5401 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gttttttggc
     5461 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
     5521 ggctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
     5581 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtac
     5641 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
     5701 catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc
     5761 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcgacaa agcaataagg
     5821 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg
     5881 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
     5941 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
     6001 cagagtaaat tcataatagt cgtgctgatg cagcccgctg atactctcag ttttgttata
     6061 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
     6121 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
     6181 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
     6241 ggaccagcac cagactcact ttcatcttaa cttcgcccgc atcgccttca atcagtttcg
     6301 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gcttttgctg
     6361 cagcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc
     6421 cggagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
     6481 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
     6541 ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt
     6601 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaaggttgc
     6661 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
     6721 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
     6781 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
     6841 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
     6901 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
     6961 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
     7021 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
     7081 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
     7141 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
     7201 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
     7261 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
     7321 ggaaagaata ataaagccgg gaattaatgc cgagaaggaa cgcgatactg aagcaggtac
     7381 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
     7441 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
     7501 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
     7561 ccccgccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
     7621 tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
     7681 gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
     7741 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
     7801 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
     7861 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
     7921 atacccttaa aaaccgcaat ttaaatattg cggtattgat ttatgaaata actctttgac
     7981 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
     8041 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
     8101 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
     8161 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
     8221 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag aggtagacat
     8281 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
     8341 tatctgtcag ccagacactc cgcaagcctt aacctgcttc catgctctgg gtaacttgcg
     8401 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
     8461 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
     8521 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
     8581 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
     8641 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
     8701 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
     8761 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
     8821 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
     8881 gatcataagc cgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
     8941 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
     9001 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
     9061 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
     9121 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
     9181 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
     9241 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
     9301 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
     9361 ccaggaaact ggcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
     9421 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
     9481 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
     9541 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
     9601 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
     9661 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
     9721 caaacgccag ggtaaacagt tgctgacggc attaggttgc ccggaacacc tttataagaa
     9781 agccccaacg gccgatctgg aagatgatcg cccttctctg ccagatgaag tggcactcgg
     9841 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacgtacctg aacaggtcgc
     9901 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaccgt
     9961 tttcgatgat ttctggaaaa agtaataatt tgcacataat ggtttgtgcg aaaaagagta
    10021 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    10081 aatatattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    10141 gtcatcgacc cgcctctttt ttaatcgctt cccgcctgtt acactggata gataaccagc
    10201 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    10261 caatttatga atatcccgaa catttacgtt cattccttaa tgacttaccc acccgaccag
    10321 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    10381 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    10441 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    10501 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    10561 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    10621 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    10681 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gctgggactg gaaccgttaa
    10741 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    10801 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg
    10861 tgtgttggcc ttggcaaggg gcggtggcgc tgaaagaaca gcacccggaa atgactcaat
    10921 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gacgcgacaa
    10981 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    11041 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    11101 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    11161 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    11221 aatcacaaac actaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    11281 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    11341 tgccgtcagt ttcgctttta ccacctggtc cgccgcaaag gcaaaaggct gtaacggttt
    11401 taaggtatgg ttaaagtggt ctgcgctttc aaggaacatc tcgccgcctt ccagcaacgt
    11461 cactatcctt tccataccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    11521 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    11581 accggcagca tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    11641 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa
    11701 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    11761 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg
    11821 gtcttaagtt ttttggctga aagattattc agcagttgca ggcattttac cttttgccgc
    11881 tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct gttccggcgt
    11941 caggatgttg taaattttgt tctgggtttc catgtgcgcc agcatgttag ctttgcgctg
    12001 ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat cgctggcaac
    12061 gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt cacgctggcc
    12121 tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat
    12181 catgtcctga tgcggaccga acttgccttt gtggtgcata atcggcttcg cgtcagccgg
    12241 tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag ccagggtaga
    12301 ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca gttatttatt
    12361 acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact ttagcgtcaa
    12421 ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc caattatgga
    12481 gaaaaaaaga aaatttgatg gagagtgatg agagaatatt acaacacgat gattttgcag
    12541 agattatgaa gaactatacc ggatgactgg tgataaataa agcaaataac caggattaat
    12601 ctgtattaat ttataagaaa gcaacttaat acccgcagag tgatttctgc gggtaagtat
    12661 tagcttattt tttcgagcat taatcccgcg cgtaatccca acgctaccaa cggattaggg
    12721 aataacacat actctacatc atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc
    12781 aatgttcctt tctcaaacgg cataaaattc agcgtgtcac ttgccatatg catttcgaag
    12841 gacggcgagt ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg tgttctcacg
    12901 ataccgacac tctcaccaga tagcagcgca gcaattgcgc tggcagttac tgcaaactgg
    12961 cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt ccagcgtaca ggccagcgcg
    13021 ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag gttcctgatg gaacaccagc
    13081 gcctccagcc ccgccgcacc cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt
    13141 tgcggtaata caccgaactg cggatgcaag gagccacgaa ttgcggtatg tagatcaagg
    13201 tgccagcgca cagattcttt gccctggtca taaaaatctt ccaggcactg ttccagttcg
    13261 cgcgcccgac aggtttctcc gctttcagca aatagctgcc aacgaccgcc aaacattcga
    13321 ttcatatcgc tatggcaata acgtttccct tgcttcagcg caggaggatt cccgaggatc
    13381 accagcaacc gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc
    13441 agcatctcca caggtgccgt ctcattaccg tgtattcccg ctgaaatcac cagtgcgcct
    13501 tgcggtggcg ttaatggcgt cagttccagc acaccatcgc ccagccagcg ccagcgaacg
    13561 ccgttgattt ctcgctcggt gataaccggt tttttacccg ttaaggtcag agcaagaaaa
    13621 ttatccattg ccccctccct cgcgctggaa cggataaacc gaaccgagat tcagtaattg
    13681 gctcaataca tccagtgctt cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc
    13741 agcagtaagg cgatcgcggt agtaacgatc cacccagtca ttgagcgcat taaacagcgt
    13801 atcgttcatc atcaccgccg gattcaccgc ccggcgttct tcttctgtca ataccacccg
    13861 caaccgcagg cacgccgggc cgccgccatt cgccatgctt tcacgtaaat caaagacttt
    13921 tagttcgcta atcgggttgt cagcggcaag gagttcattg agataacccc atactccggc
    13981 gtgttcccga cactcctgag gcagcaccaa catcatagaa ccatcatcgc ggctcaacag
    14041 ttggctgtta aacagatagg tagacaccgc atccgacacg gaaacctgag ttgccggaac
    14101 ttctatcgcc ataaaaccgt tcacccgcgc acgcaggttt gccagtaact gtgactggcg
    14161 agcgaacgcc tgttggtggc aaaacagcac ctggcggtta ctcacggcaa tcacgtcatt
    14221 atgaaaaacg ccctggtcga taacgtccgg gttttgctgg gcgaaaatca cctgttgggg
    14281 attcacctga ttcagccttg ccaccgcctc gctggcttcg cgagtctgtc gcgccggata
    14341 acgggaaggc cgggtatcat tgccttcttc tcgcccgtag acaaaaagtt gcatacccgg
    14401 ctcaccgtaa tgaccgccga gacgattgtg gtttgccgcc ccctcatcac cgagcaacgc
    14461 tacctgcggc aacgccgaat gaacgctaaa tttctcttcg tcgttaaaaa tcgcttttaa
    14521 aagtgattcg gtgacgtgcg cttccagcga gcggtgaaat ttattgttca ggttggcaac
    14581 ggtgagatgc actttgccat ccagcgtatc ggcagatggc gcgatcgttg ccgcattggc
    14641 tacccacatt ggcgaagcgg aactgacgct ggaaagccag tgcggtgcct ggcgtgcaac
    14701 tttttccagt acctgctcat cgctaccact gaatcccaac tgacgcagca ccggaataaa
    14761 cggacgctcg tgcggcggga tcacggcctg ggggaatccc gcatcggcaa gggctttcat
    14821 tttcagtaag ccctgttttg ccgcctgtcg cgggttagac acctgaaaac ggtgacgggt
    14881 agaggcttca ttaccaaacg acaggccagc gtaatgatgc gtcagcccta ccagcccgtc
    14941 gaaattaact tcccaggcgt tcatcgcacc acctcatcgg aaaaatccag cccggggtta
    15001 agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg ccataggcca tgcgcagtaa
    15061 tctgcggcat accaggcgct ggggcgatgg ttaccggatg caccaatgcc gccgaatggc
    15121 gcggtactgg cagcaccagt aagcggtttg ttccagttaa caatccccgc ccgcgcctcc
    15181 agcaacagtt gatcgaattt ttcccgctcg ggggaaacca gaccgcaaga gagtccgaag
    15241 cgagtgttat tcgccattag aatcgcttcc tcgaaagaat cataacgcca gacgcgcaat
    15301 aacggtccga acacctcttc atctggtacg ccagcaacgc ctgtcatttc aatgatcccc
    15361 ggcgtcagca acgatgtctc tgattgtaat aagcgcggcg caagcagggt tcgtccgccc
    15421 atcgcttcca gttgctgcca ggcagtaacc acctgctgtg cggcctgttc agaaatcagc
    15481 ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg gcgttaatcg ctggctgacg
    15541 gcaaccagac gagcaagaaa cgcatcgccc tgcgctccgc ttttgagcaa taaacggcgg
    15601 gcgcaggtgc agcgttgccc ggctgtgaca aacgccgact gaatggtcag atggacagcc
    15661 gcgtcgatat ccgccacctc atcgataatt agcggattat taccgcccat ctcaagggcg
    15721 agaattttct ccggctgacc ggagagctgg cgatgcagct ggtagccggt attggcgcta
    15781 ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca gcgcctgacc cgtttcacgc
    15841 ccgccctgca ccaggttcag tacgcccggc ggcaagccag cctgctgcca taaacgcatt
    15901 accgcttcgc cactccacgg tgtcagttcg ctgggtttaa agataatggt gttacctgcc
    15961 agcaatgccg gaacgatatg tccgttcggc aaatgaccag ggaaattata cggcccaaac
    16021 accgccagca cgccgtgcgg gcgatgtcgc aggctcgccg caccgtccgg catttcacta
    16081 cgctgctcgc cggtacgaac gtgatacgcc ttaattgata tcgcgatttt attgatcatc
    16141 gccgtcactt cggttgccgc ttcccagcgc ggcttacccg tttctctggc aataatcgcg
    16201 gttaattcgc ctttattcct ttccagcaat ccggcaaagc gttcgacaac gacctgacgt
    16261 tcagccaatg agagccgcgc ccagcgcgga aacgccgcac gggctgcccg acaagcctgt
    16321 tcgacctgag cggcatcggc atcattgcct tgccataaca cctcgcccga taccggatta
    16381 cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca taaagtcatg
    16441 ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg atcccctgcg tggcatttga
    16501 gggcatccag ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg gtacgcgcca
    16561 gcaccacgcg gaaatggtga taattttcat tggcgaccag gcaggccgga aaatcgccct
    16621 gcgcaggctg cccttctgcc acttccacca gccgactttt acgaatggcg cgcacgcggt
    16681 cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg cggtaacgaa
    16741 aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc tgaccgatga
    16801 cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg atgtttcggc atcagttctg
    16861 caataaatgc cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa tccatcgaaa
    16921 agaagcgttt accgaggctt tgccagaacg gtgaatagcc gtgttcgtca atcaccccgc
    16981 gcatttcagc aaccactttg tcattaaact tgtcgcgaaa agccgccata aacataaagc
    17041 gcgatttcga cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg agaaacagcg
    17101 tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa cagcgtcggc aatgcgttat
    17161 agacattcag ctcttttgag gcgtgaacca acgtgccgac gcgatagttg taccagggat
    17221 cgttcagccc aaccgccacc tcaatggcac aaatccccgc cacggtgcct gtctcgctat
    17281 cttccagcac gaacacatag ccctgctcac ttttgggcag ttcgccttgc caggttttga
    17341 ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc gggaagcgac gtcaggccgc
    17401 cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc tgatcgctca acgggacgga
    17461 tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa agcgatccag
    17521 tccggtcgtc acctcttctt cgctgacatt gagcgcaggc gcaaaacgca ccacgttgcc
    17581 acccgcaatc agtaccatca cgcctgcttt cgccgcttcc tgagagatct gtttcgcttg
    17641 cccggcgtaa tcggcattca gtacacagcc aatcagcaaa cctaagccgc gaacttcact
    17701 gaacaaacca tagcggtgat taatagtatt aagacgctca acaaaccagt cgtgacgctg
    17761 tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc agcactttgc ctgccaccgc
    17821 cgaggccagc ggattaccgc cataggtggt gccatgagtg ccaacggtca tcacgcgggc
    17881 gcactcttcg gttgccaaca acgcaccgac cgggaaaccg ccgcccagcg ctttggcggt
    17941 agttaacaga tcaggcgtca cgccgtagtg catataggca tacagttccc cggtgcgccc
    18001 gacgccggtt tgtacttcat caaaaatcaa cagcgcattg tggcggttac acaattcacg
    18061 cagaccttgt aaaaacgcgt tgctggctgg caccacaccg ccttccccct ggatgggttc
    18121 gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg gcagagttaa tatcgttata
    18181 tgcagcatga cgaatatccg ccggcagtgg cgcaaaatcc tgtgaatagg ctggctgccc
    18241 acccgcactg acagtaaaca gcgtgcgacc atgaaacgca tttttgaacg ccacgatgcc
    18301 gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta gcgccgcttc
    18361 gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg cgtcgatcaa
    18421 ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat gccagaactt
    18481 actcgcctgt tcgttcagcg cttcacgcag ttccggatgc gcatggccca gcgcgttcac
    18541 cgcaatgcca cccgcgaagt cgatatactc tttccccagc tgatcccaca agcgcgaacc
    18601 ttcgccacgt accggtataa agggtgccgg agcgtaaaca ggtatcatcc attcatcaaa
    18661 gttttcacgc gtaattggct gagacatagc gacctctaca gtaaataatt cgttatttat
    18721 atgttaacaa taagtaatgt ttgcgctgta aatgtagatt gcagggttcg tgccagccag
    18781 tgataaaagt gcataaacgg cggaggctaa ctggaaatca aggagttata accaaaccat
    18841 atgcatttaa agtgcatata aagtgaatac gtttgcgatg tgggtgaata aaaagaataa
    18901 aaaacgcaat gttatgcaga agtaaaatat aattctggaa ttgtgatcat tgacgaaatt
    18961 tactggaaat tactgcgcca ttctgacgca gcgcgcacca aaagcgggca ttttttgcgc
    19021 catcgttgac atcattaaca accatcgatc aaatcactta acaacaggcg gtaagcaacg
    19081 cgaaattctg ctaccatcca cgcactcttt atctgaataa atggcagcga ctatgaaatt
    19141 tgtctctttt aatatcaacg gcctgcgcgc cagacctcac cagcttgaag ccatcgtcga
    19201 aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa gttcatgacg atatgtttcc
    19261 gctcgaagag gtggcgaagc tcggctacaa cgtgttttat cacgggcaga aaggccatta
    19321 tggcgtggcg ctgctgacca aagagacgcc gattgccgtg cgtcgcggct ttcccggtga
    19381 cgacgaagag gcgcagcggc ggattattat ggcggaaatc ccctcactgc tgggtaatgt
    19441 caccgtgatc aacggttact tcccgcaggg tgaaagccgc gaccatccga taaaattccc
    19501 ggcaaaagcg cagttttatc agaatctgca aaactacctg gaaaccgaac tcaaacgtga
    19561 taatccggta ctgattatgg gcgatatgaa tatcagccct acagatctgg atatcggcat
    19621 tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa tgctctttcc tgccggaaga
    19681 gcgcgaatgg atggacaggc tgatgagctg ggggttggtc gataccttcc gccatgctaa
    19741 tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt ttgacgataa
    19801 ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat gttgcgtaga
    19861 aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg cccccgtctg
    19921 ggcgaccttc cgccgctaat ttagcagctc tcctggctca aactgggtca ggagaattaa
    19981 ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc tttgttatac
    20041 cgtcactgcg tttttagttg tctgaccact tctctattat caagtttgat ataggaaact
    20101 ccacgatgaa cgctgagcgt aactttctgt ttgcctgtct tatttttgcg ctggtcattt
    20161 acgctatcca cgctttcggt ttattcgatc tgctcaccga tttaccccac ttacagacac
    20221 tcattcgcca gagcggattg ttcggttata gcctctatat tctgttattc atcattgcca
    20281 ccctctttct gttaccagga agcatactgg tgatcgctgg aggaatagtt tttggcccgc
    20341 tcttagggac actactctca ttaattgccg caacgctggc ctcctcgtgc tcattcctgc
    20401 tggcgcgctg gctgggacgt gatttactgc tgaaatacgt gggccatagc cataccttcc
    20461 aggccattga aaaaggcatt gcgcgtaacg gtattgattt tcttattctg acccgcttaa
    20521 tcccgttgtt tccttacaat attcaaaatt acgcttacgg attaaccacc atcgccttct
    20581 ggccttatac ccttatttcg gcactcacga ccctgctcgg tattgttatt tataccgtga
    20641 tggcaagcga tctcgccaat gaaggcatta cgctgcgctt tattttacaa ctctgtctgg
    20701 cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact ctacgcccga cacaaacatg
    20761 tggatctgtc tgcttcgcgc cgcagcccac ttactcaccc aaaaaatgaa ggatagaacg
    20821 atgtcgcaac attattcagt gtcatggaaa aaaggactgg ctgcactctg cttactggct
    20881 gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg cggcaaaagt ggaatacgac
    20941 ggactttcga acagccaacc gctgcgcgtc gatgccaata accatacggt aaccatgctg
    21001 gtgcaaatta atggtcgttt cctcaccgac gacactcgtc acggtattgt gtttaaagat
    21061 ggctccaacg gacataaatc gctgtttatg gcttatgcga ccccgaaagc attttatgaa
    21121 gccctgaaag aggcaggtgg tacgccgggc gaaaacatga cgatggataa taaagaaacg
    21181 actcatgtca caggcagcaa actggatatt tcggtcaact ggcaaggggc ggcaaaagcg
    21241 tattccttcg atgaagtgat tgttgacagt aatggcaaga aactggacat gcgctttggc
    21301 ggtaatttaa cggcagcaga agagaagaaa acgggttgcc tggtgtgtct ggatagctgc
    21361 ccggtcggca tcgtcagcaa tgcaacatac acttatggtg cggtggaaaa acgcggcgaa
    21421 gtgaaattca aaggcaatgc ctcagttctc ccggcggata acacgctggc aacggttacc
    21481 tttaaaatca ccgaataaag ccaggataaa ggatgatgaa gatacaatcg cgtaaaatct
    21541 ggtactaccg tataaccctc atcatcctgt tgttcgctat gctgctcgca tgggcgctgc
    21601 ttcccggcgt ccatgagttt atcaatcgca gcgttgcggc gtttgccgcc gtggaccaac
    21661 agggtataga acgctttatt cagtcttacg gcgcactggc ggcagttgtc tcgttcttgt
    21721 tgatgatttt acaggccatt gctgcaccgc tacctgcgtt tttgatcacc tttgccaatg
    21781 cgtcgctgtt tggcgcgttc tggggcggct tgctgtcgtg gaccagttcg atggccggcg
    21841 cggcgctgtg cttttttatc gccagagtga tgggccgcga agtggtggaa aaattaaccg
    21901 gcaaaaccgt gcttgacagt atggacggct ttttcactcg ctacggcaaa cacaccatcc
    21961 tggtctgtcg gttattgcct tttgtccctt tcgatccaat cagctatgct gccggtctga
    22021 cttcaatacg ttttcgctcg ttttttatcg ccaccgggct tggtcaatta ccggcgacta
    22081 ttgtttattc ctgggcgggc agcatgttaa caggcggtac tttctggttt gtcaccggac
    22141 tgtttattct gtttgccctg accgtggtga tttttatggc gaagaaaata tggcttgagc
    22201 gccagaagag gaatgcctga tgggtttacc gccgcttagc aaaattcctt tcattttacg
    22261 tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg ctaagcccga ttcgctggtg
    22321 ggggcggatc ccgtttatct tttatttggt gtcaatgttt gttgggtggc tggagcgcaa
    22381 acgctcaccg ctcgatccgg tagtgcgatc gcttgtcagc gcgcgcattg cgcaaatgtg
    22441 cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc gccgagcgta ccggcagcac
    22501 cgataaactg ctggcagtgg ctgactggcg gcaaagcccg ctcttcagcg atgaagaacg
    22561 gctggcgctg gagtacgccg aagccgcaag cgtaacgccg ccaacggtcg atgatgccct
    22621 gcgcacccga ctggctgcgc attttgacgc tcaggcgctc accgaactga cggcattgat
    22681 cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg gacattcccg ctcaggggct
    22741 gtgccgtatt ccggaaaaac gttcttaagg agagaagatg cgccattgtg ggtggttgct
    22801 gggattgtta tcgctgtttt ctctggcaac acatgccagt gactggcagg aaattaaaaa
    22861 tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc ggcgataccg caattaaccg
    22921 ctatctcgac tgggttagcg gcgagatgaa aacccattac gctataaacc tgaagattgt
    22981 tcgtctggcg gatgccgcag acgcggtgaa gcgcattcag accgaagctg ctgccggacg
    23041 taaaacgggc ggctcggtgg atctgctctg ggtgaacggc gaaaacttcc gcaccttaaa
    23101 agaggccaat ttattacaaa cgggctgggc ggagactctg cccaactggc gctatgtcga
    23161 cacacagctg ccggtgcggg aagatttttc agtgcctaca gaaggggctg aatcgccctg
    23221 gggcggcgca caactgacgt ttatcgcccg ccgcgatgtt acgccacagc caccacaaac
    23281 gccgcaagcc ttactggagt ttgctaaagc caatcccggc acggttacct atccgcgccc
    23341 accggacttt accggcacgg cgtttcttga acagttgctg attatgctga cgcccgatcc
    23401 cgccgcgtta aaagttgccc cggagaaagc cactttcgcc cgcgtcactg cgcccttgtg
    23461 gcaatatctt gacgcgctac atccctattt atggcgcaaa ggaaaggatt tcccgccttc
    23521 gcccgcgcgg atggatgctc tgctgaaagc cggcacattg cgcctgtcgc tgacctttaa
    23581 ccctgctcat gcccagcaaa aaatcgccag cggcgatttg cctgcaagca gttacagttt
    23641 tggttttaac caggggatga ttggcaacgt gcatttcgtc accattcctg ctaacgcaaa
    23701 tgccagtgct gcggcgaagg tggttgccaa cttcctgctc tcacccaatg cgcaactgcg
    23761 taaagcggat cccgctgtct ggggcgatcc ttctgttctc gatccgcaaa aactgcctga
    23821 tgggcagcgc gaaacattgc aatcaagaat gccgcaagat ctgccgccgg tactggctga
    23881 accgcacgca ggttgggtaa atgcgctgga acaagaatgg ctacaccgtt acggtacgca
    23941 ttaatctttt tgctgtgggc gatggtggcg gtgatttatg caccactgat cccggcagct
    24001 ctcacgctga tatcgcctgc tttgtcgttg acacactggc aggcgttatt tgccgatcca
    24061 cagttaccgc aggcattact ggcaacgctg gtgtcgacaa ccatcgcggc ggtcggggcg
    24121 ttgttgattg ccctgctggt gattgtggcg ctgtggcctt ggccgaaatg gcagcgtatg
    24181 tgtgcccgtc tgccgtggct gctcgccatt ccccatgtgg cttttgccac cagcgccctt
    24241 ctgctctttg ctgacggagg gctgctttat gactatttcc cgtatttcac tccgctaatg
    24301 gacaaattgg gtattgggct gggcctcacc cttgcagtga aagaaagcgc atttctgctg
    24361 tggatcttag cggcagtatt gagcgaaaaa cggctgttgc agcaggtcat tgtgctggat
    24421 tcgctgggct acagccgctg gcaatgcctg aactggctgc tcttgccctc cgtcgcacct
    24481 gcactggcaa tggcgatgtt ggcgattgtt gcctggtcgc tttcggtcgt ggatgtggcg
    24541 attattcttg ggccaggtaa tccaccgacg ctggcagtaa ttagctggca gtggttaact
    24601 cagggcgacg cggatcaaca aacgaaaggc gcactcgcca gtctgctgct gatgctgtta
    24661 ctcgccgcct acgttttgct gagctatctg ctatggcgca gctggcggcg cactattccc
    24721 cgcgtagatg gcattcgcaa gcctgccaca cctttattac caggcactac gctggcgagt
    24781 tttttaccct taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg
    24841 acgatcaata gtgaagcact catcaacagc ctgacaatgg ggctggtggc gacattcatc
    24901 gctttgctcc tgttactgct gtggctggaa tgggggccac agcgtcgcca gttgtggctc
    24961 tggttgccca ctctattgcc cgctctgcca ctggtggcgg gccagtacac gctggcgcta
    25021 tggctgaacc tggatggaag ctggacggcg gtggtctggg ggcatctgct gtgggtaatg
    25081 ccgtggatgc tgtttatcct gcaacccgcc tggcagcgca ttgattcacg gttaattttg
    25141 attgcgcaaa cactgagctg gtcgcggacc aaaatcttct tttacgtgaa atgtccgctt
    25201 atgctacgcc ctacgctgat tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat
    25261 atgccgacgc tgtggctggg cgcggggcgt tttccgacgc tcaccactga ggcggtggca
    25321 ttaagcagcg gcggcagcaa tggtattttc gccgcccagg ctttatggca actgctatta
    25381 ccgcttatta tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa
    25441 ggactccgct aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc
    25501 tgacaaacgt taactttacg gtggataaag gtgacattgt cacgttaatg gggccgtctg
    25561 gctgtggaaa atccactctg ttttcatgga tgattggtgc actggctgga cagttttctt
    25621 gtacaggtga gctatggctc aatgagcaac ggattgacat gctacccacc gcacagcgtc
    25681 agattggcat tcttttccag gatgcactgt tatttgacca gttcagcgtc gggcaaaatt
    25741 tactgctggc gctaccgtcg acgctcaagg ggactgccag gcgcaatgcc gtgaaagacg
    25801 cgcttgatcg agcgggactg gctgaaactt atcaccagga ccctgcgaca ttatcaggcg
    25861 gtcagcgagc gcgggtggcc ctgctgcgcg cccttctcgc tcagccaaaa gcgttactcc
    25921 tggatgagcc attcagccgt cttgatgtgg ctctgcgcga taattttcgc cagtgggtgt
    25981 tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt aacgcacgat ctccaggatg
    26041 ttcctgctga tagttctgtt ctggatatgg agcagtggtc agaaaattac aacaaactgc
    26101 gataacgcaa agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa actacaatgt
    26161 cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg
    26221 cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct tacacttgac cagcttcaac
    26281 aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt ttataacggc tggccacaaa
    26341 ccttaaatgg cccttctggt catgaacctg ccgccttaaa cctctctgcc agctggctcg
    26401 acaaaatgag caccgaacag ctcaacgcgt ggatcaagca acataacctg aaagccgatg
    26461 ccccggtggc gctgtacggt aatgacaaag atgtcgacgc cgtcaaaacg cgactgcaaa
    26521 aagcaggttt tacgcatatc tccattctga gtgacgcgct aagcgaacct tcccgtctac
    26581 aaaaactgcc gcactttgag cagctggttt atccgcaatg gctgcacgac ctgcaacaag
    26641 gtaaagaggt tacggcgaaa cctgccggtg actggaaagt cattgaagcg gcctggggcg
    26701 ctcctaagct ttaccttatc agccatattc ccggtgctga ctacatcgat accaacgaag
    26761 tggaaagcga accgctgtgg aacaaagttt ctgatgaaca actaaaagcg atgctggcaa
    26821 aacacggcat tcgccatgac accacggtca ttctgtacgg gcgtgacgta tacgctggag
    26881 cgcgtgtggc gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg ctggatggcg
    26941 gctggcaaac ctggtccgac gcgggactgc ctgttgagcg aggaacgcca ccgaaagtga
    27001 aagcggaacc ggatttcggc gtgaagatcc cggcacaacc gcagttgatg ctcgatatgg
    27061 aacaggcccg tggactgctg catcgccagg atgcatcgct ggtgagcatt cgttcgtggc
    27121 cagaatttat cggtacgacc agcggttaca gctatattaa accaaaaggt gaaatagccg
    27181 gagcacgttg gggacacgct ggtagcgact cgacgcatat ggaagatttt cataacccgg
    27241 atggcaccat gcgcagcgcc gatgatatta ccgctatgtg gaaagcgtgg aatatcaaac
    27301 cagagcagca agtttcattc tactgcggca ccggctggcg cgcgtccgaa acctttatgt
    27361 acgcacgcgc catgggttgg aagaatgttt ccgtgtatga cggcggctgg tacgaatgga
    27421 gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg cccggacagt agcaaataac
    27481 attgcaatta ctgacgctgg tgcgacttca gcgtcagata cccactccac acccgcgtaa
    27541 aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac catgcaggga
    27601 ataaacatcc cagcacaaac agtaagatcg tttctgtgcc ttcggttaac ccgcccagat
    27661 aataaaacga tttatgcgca taaccgggat tatcaatctg atgtttcgcc gccagcgcag
    27721 caaaagcgag aaaactgctg cctgtgccga taaacgcaaa cagcagccag ccgcctgcca
    27781 gggcgttttg ctgcggtgcg gcgaggataa acccaaaagg taccagcgcg taaaaaagaa
    27841 aatcgagaga aatatcaaga aagccgcccg cgtcggtaag ccctctgcgc cgcgccagcg
    27901 caccgtccag accatcaagc agcctgttca acaaaatgac gacaagcgcc gccagatacc
    27961 agcccagcgc cagaaaaggc agtgccagca cgccgatggc aaatcccact aatgttaaac
    28021 catctggcgt aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac aacggtttaa
    28081 tccggggatg aagatggcgg tctagcacag gcactcctta aatataaagc ctttctgatt
    28141 gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta atttatgaaa
    28201 atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact cgcgcaacgc
    28261 cccgcccaga gcgatcaggc gggattatgg gagtttgccg gtggtaaagt cgagccggat
    28321 gaaagccagc ggcaggcgct ggtgcgtgag ttacgcgaag aactgggcat cgaagcaact
    28381 gtgggtgagt atgttaccag ccatcagcga gaagtttcgg gccggattat ccatcttcat
    28441 gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca ggcgctggtc
    28501 tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat tccattatta
    28561 gaggcgttta tggctttacg cgccgccaga ccagcggatt agtgctaagg gttttgtcat
    28621 cccgctggca ttgcagcagt attccctcgg ctttaattac cgcgccttca gaataatttt
    28681 tatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct gaactgaaca
    28741 cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc agtgcaatta
    28801 atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc ttaacgttat
    28861 tgtctctgct actgataacg gtagccgggt ggcaaaactt tagcgtctga gttatcgcat
    28921 ttggttatga gattactctc gttattaatt tgctttcctg ggtcattttt ttcttgctta
    28981 ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatcacata aacaacataa
    29041 gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat tctctggagt
    29101 cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg caagccgttc
    29161 gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat cgccagatgt
    29221 cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg gtatgggttg
    29281 atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt gcagttcagc tctgccatcg
    29341 gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctctccatt cttaaattcc
    29401 tcggcttcga acaaaccttc aaaaatgccc tgactaccct gccgatgggc ggtggtaaag
    29461 gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga agtgatgcgt ttttgccagg
    29521 cgctgatgac tgaactgtat cgccacctgg gcgcggatac cgacgttccg gcaggtgata
    29581 tcggggttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag ctctccaaca
    29641 ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggcagtctt attcgcccgg
    29701 aagctaccgg ctacggtctg gtttatttca cagaagcaat gctaaaacgc cacggtatgg
    29761 gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag tacgctatcg
    29821 aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc ggcactgtag
    29881 ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc aaagccagcc
    29941 gcgatggtcg agtggcagat tatgccaaag aatttggtct ggtctatctc gaacgccaac
    30001 agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac gaactggatg
    30061 ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgccgaa ggggcaaata
    30121 tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg tttgcaccgg
    30181 gtaaagcggc taatgctggc ggtgtagcaa cgtcaggcct tgaaatggcg caaaacgctg
    30241 cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc atgctggata
    30301 tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac gtgcagggcg
    30361 cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt gtgatttaag
    30421 ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa atgggcacaa ttcattgcag
    30481 ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca ggcgtttatg
    30541 agtatttaac ggatgatgct ccccacggaa catttcttat gggccaacgg catttcttac
    30601 tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc ttcaagttca gcatccgtta
    30661 actttctgcg atagcagcag atatgccagt aaagaaaccc cattagacta tttttttgat
    30721 aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt atataatgcc
    30781 aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc gaggtcatcc
    30841 atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga ttattagcgc
    30901 aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg ataacctgct
    30961 ctggggcttc ctccatgttt gctttaaagg tattggctcc atggtcgcca gaaagaaaat
    31021 actccattaa ggcacaataa ctttcgctat cttcgatacc ccattgatcc tctaaagact
    31081 cgcgtctttt gcttatgata tcgatcgagt caaagggaag cacatgatat tggaaggtat
    31141 ctttgccagg ttcaggcttt cgcggccaga actccagcgt ttcagaccat tgcttatgat
    31201 agaaccgata aggtgcgatc aattgtagcg cctgtaactt ctcgatactg agcggctcaa
    31261 tacctttagc ctgataataa tgcagttgtt ctttttttgc tttaaaaccg gcccgaacaa
    31321 taagccccat cataattaat agataaagaa aagagcatcc cgcggtaatc aggcctcttt
    31381 cattcaaacc gttggatgtt atcgctgcga acacaaacat tacagcgaca acacatgtta
    31441 aataaaaccc ccacttacaa agcagcatgg ccttattttc tttaatcatc cgttcaaaat
    31501 tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga tggttttgta
    31561 ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat aattttatcc
    31621 atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga atatgactcg
    31681 aatagcacga aagattcact cgcttacgct atcgccccgc ttccgacttc atctgctggc
    31741 ggactttttt tcgcactacg tttacgcggt gcagcctttt tcttatcagc actgccacca
    31801 ctgcccggag ccacaatgcc gcgaaactgc cgcaccggcg tacgtttggc ttgatcaata
    31861 agctgatata gcgtccccac cagcggctgc ataaagtcct gatagcgaca ctgcttttcg
    31921 ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt cgccatttct
    31981 ggcagcgaat ggaataacgc ttttccggcg tcggtagagt ggatatagcg cccttttttg
    32041 gtcagaaaac cacgcttgaa cagcagttca ataatcccgg cacgggttgc ttctgtcccc
    32101 agaccatcgg tcgcacgaag gatctttttc agatctttgt cctgcacaaa gcgcgcgatc
    32161 ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg ggtttgccgc
    32221 tctaccactt cacctttttc acacagcaac tcatcgcctt tcgccaccac aggcaatggc
    32281 gtaccgtcgt tttcttcatc gcgctctttg ctgcctaaca gcgtgcgcca gcctgcttca
    32341 gcaagaaaac gggctttagc gacaaatttg cctttggcaa tgtccagttc gataacacac
    32401 ttgcggaaca ccgcatccgg gcagaactgc atcaggtatt gacgggcaat caggttatag
    32461 accttcgctt cgttctccgt caggttgatc gcagaactcc gtgcggtcgg gatgatggcg
    32521 tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc gtatatctgg atctaccact
    32581 gactgcggca acagatccgg tgcatgaacg ctgatggcat tcatcaccgc gtggcgtccg
    32641 gcaaaatgtt cttctggcaa atagcgacaa tcggaacgcg gataagtgat tagcttgtgc
    32701 gtttcgtaca gcttctggca gatatcaagc acgttctgcg cgctcagacc gaagcgtttt
    32761 gccgcttcaa tctgtaacgc cgaaagcgaa aacggcagcg gcgcggattc tgattcccgt
    32821 ttatcgttat agctggtgac aatagccggt tgaccactaa tgcggttaac cacatgctcc
    32881 gccagtggac gatgtaacaa gcgcccttct tcatcctggt acggttcaca cgcttcgctc
    32941 ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc tttgacttca
    33001 aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac cagcccgagc
    33061 acgggtgtct gcacgcgtcc cacggaaagt acgccctgat aaccggcatt gcgaccgaga
    33121 atggtatacg cacgggtcat attgatgccg tacagccagt cggcacgcgc tcgcgccagc
    33181 gcagaaacgc acagcggtac aaactcactg ttggaacgaa gacggtcgat cgcccgctca
    33241 accgcctgcg ggttcaggtc gtttatcaag caacgctgca cctgctggcg cttttccggt
    33301 gccagttgca gatagtccag cacttcatcc accagcaatt gcccttcacg atccgggtcc
    33361 ccggcgtgaa cgatttcgct ggcttcatgc aggaagcgtt tgatgacgtt aagttgtttg
    33421 gtcacggagg gtcggggctg taattgccac ttttccggga caatcggcaa atccgcaaga
    33481 ttccagcgcg catagcggct gtcgtaggcg tctggctgcg cctgctcaag caggtgaccg
    33541 atacaccagg tcaccacctg accattaccg cactcgataa agccatcgcc tttacggtgc
    33601 ggtttgggca ggacatcagc aatggcgcgc gccagactcg gtttttcggc aataaacaac
    33661 cgcattgaat taatggatct caaccatggc acgaccgccg cgcgctggca ccagttcgcc
    33721 aatcgccgtc agttcaatgc caaactcggc ggctgtagtt ttaacctcat tttctgcttc
    33781 cggcatgacc gccagcagca aaccgccaga agtttgcgga tcgcacagca gatcgcgcac
    33841 ttcacgcggc atttcaccca tcagatgacc gtagctggca aagttacgtt cggtgccgcc
    33901 aggcactgcg cccaacttaa tgtactcttc aacccccggc aattttggga tcgcgtcata
    33961 gtcaacgcgc gcctgcacac ctgcgccctg acacatttcg ctcaaatggc ccagcagacc
    34021 aaaaccagta acgtcggtca tcgcttttac gccttcgatg ttggcaaagg acgcacctgc
    34081 gatgttcatc cggcacatca cttccgtcgc cagtccctga tgttctggtt tcaacagtga
    34141 ttttttctca gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga acagtttgca
    34201 tccggcttgt gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc ccgttaccgc
    34261 cagaccaaaa atcggctccg gcgcatcgat ggagtgaccg ccagccagcg caatacccgc
    34321 ctgacggcat gcatagcgtc caccttcggt tacttcgcgg gcaatttctg gggaaagttt
    34381 gttaatcggc cagccgagga tcgcaatcgc cataatcggt ttgcccccca tcgcgaagat
    34441 atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat caacgatcgg
    34501 cataaagaag tcggtggtac tgataacgct ggtgccattg cccagatcgt acaccgccgc
    34561 atcgtcgcgg gtctcattac ccacaagcaa attcggatca acaaacttcg cctgctcact
    34621 gtgcaggatg gtttccaaca cttttgggga aattttacag ccgcaaccag ctccgtggct
    34681 gtattgggtc aaacgaatcg agttctcgct catggacatc tcctgtcaat gcaatccggg
    34741 tatggtaacc ctcattccgt gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc
    34801 tcacaatcca gacagtttcg cgacaattat cagaaataag ttacaaacgg cgtcgggtcc
    34861 gggacgttaa tcgacgtaga tgctttcaac tgcggcgtgc cgaggtagag aaaaccgaca
    34921 attttatcct gctcacggca accgaatgct tcacgcacta ccggactttc agttaatgcg
    34981 ccactgcgcc agatgccgcc aaatccttgg gcaacggctg ccatttgcat cgccatgacc
    35041 gcgcatccgg cagacatttc ctgttcccat cgcgggactt tatgattttc ttcgcatttc
    35101 gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt gtcgatagct
    35161 ttgtcatcac taccggcagc aatcgccccc tgttccagta cggcgctgaa acgctcgcgc
    35221 ccttcccctt caatcacaaa aaaatgccac ggttgcatgg acttatggtc cggcgcacgc
    35281 atacccgcac gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc agccaggcgg
    35341 gaggcgctac ggcgattgat caatagttcg agtgcatcca tttgattaac tcctgtcgtg
    35401 atatttattc acaaaattaa cacgagagtg gattttgtta cagcacagtc cgcaattcct
    35461 gctgacaagt gccggttggg tcattacgat aaccacatct attgcgcctg tgacaggtgt
    35521 gaccttaagt tgggagaata catgcgaacc ctttggcgat ttattgccgg attttttaaa
    35581 tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt ctttattttc
    35641 ctcgtactgg ttggtgtggg gatctggatg caggtcagtg gtggtgattc gaaagaaacg
    35701 gccagtcgtg gcgcactgct gctggacatt tctggtgtga tcgtcgataa acccgacagt
    35761 tctcagcggt ttagtaaatt aagccgccag ctgcttggtg ccagttccga tcgtctgcag
    35821 gaaaactcac tgtttgatat cgtcaacacc attcgccagg cgaaggacga ccgcaatatc
    35881 accggtatcg tgatggatct gaaaaacttc gcaggcggcg accagccgtc tatgcagtac
    35941 atcggcaaag ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc cgttggcgag
    36001 aactacagcc aggggcaata ttatctcgcc agtttcgcca ataaaatttg gctgtcaccg
    36061 caaggcgtgg ttgatctgca cggttttgct accaacggtc tgtactacaa atcgttgctg
    36121 gataagctga aagtttccac ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa
    36181 ccgtttattc gtgatgatat gtcaccggca gcccgcgaag ctgacagccg ctggattggt
    36241 gagctgtggc aaaactatct gaatactgtt gccgctaacc ggcagatccc tgctcagcag
    36301 gtattccctg gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg cgataccgcg
    36361 aaatatgcac tggaaaacaa gctggtcgat gcactggcat cgagtgccga aatcgaaaaa
    36421 gctctgacca aagagttcgg ctggagtaag actgataaaa attatcgcgc catcagttat
    36481 tacgattacg cattgaaaac gccggcagat accggtgaca gcatcggtgt cgtcttcgct
    36541 aatggcgcaa ttatggatgg cgaggaaact caggggaatg ttggcggtga taccactgcg
    36601 gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct gcgtgttaat
    36661 agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg
    36721 gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg tggttactgg
    36781 atttccacgc cagctaatta cattgtggct aaccccagca ccctgaccgg ttctatcggt
    36841 atcttcggcg tgatcaccac cgtagaaaat agtctggatt cgattggtgt tcatactgat
    36901 ggtgtctcaa cttcaccgct ggcggatgtt tctatcacca gggcactgcc gccggaagcg
    36961 cagcagatga tgcaattaag cattgagaat ggctataaac gctttatcac gctggttgct
    37021 gatgcgcgtc attcgacgcc ggagcaaatt gataaaatcg cccagggcca cgtctggacc
    37081 ggtcaggatg caaaagctaa cgggctggtc gatagtctcg gggatttcga tgatgcggtt
    37141 gccaaagcag cagagctggc aaaagtgaaa cagtggcatc tggaatacta cgttgatgaa
    37201 ccgaccttct tcgacaaagt gatggacaac atgtctggtt ctgtccgggc aatgttgcca
    37261 gatgcgttcc aggccatgtt acctgcaccg cttgcctcgg tagcctctac cgttaaaagt
    37321 gaaagcgaca agctggccgc gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc
    37381 tgcgccaacg tgcgttaagt cttgtactga gtggccgaca gatcgtcggc cacattattt
    37441 tttacctcga cgaatcctct tcccgctgtc tcgacccata tttccttatc cgcatagtat
    37501 caggtgcgct cctcctgcct cacgtatata cttttgctct ttcgatatca ttcatatcaa
    37561 tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg ggatgcagcg
    37621 ttccgagcag ggttatattc cggtgtcagg tcatctacaa cgccaactgg cgctgatgcc
    37681 ggaattccat cgcccggaga tgccagattt caccattcat gaatatacgc cgttgatgga
    37741 ttcttctgat atgacgccgg aagactggca gcatattgct gaagatatta aagcgcacta
    37801 tgacgactat gatggttttg tcattctaca cggtaccgac acgatggcgt ataccgcctc
    37861 tgcgctgtcg ttcatgctcg agaatctcgg taaaccggtc attgtgacag ggtcacaaat
    37921 cccgctggct gagttacgct ctgacggaca aattaatctg ctgaatgcgt tgtacgttgc
    37981 ggcgaattat ccgatcaacg aagtaacgct ctttttcaat aaccgattgt atcgcggcaa
    38041 ccgcactacc aaagcccatg ccgatggttt tgatgcgttt gcctctccaa accttcctcc
    38101 gttactggaa gctggtatcc atattcgtcg tttgaatacg ccacccgccc cgcacggtga
    38161 aggggaatta atcgttcatc caatcacgcc acaaccaatt ggcgtagtga cgatttatcc
    38221 agggatttct gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag cattgattct
    38281 gcgctcctat ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg aattacaaga
    38341 agccagcgat cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg gtaaagtgaa
    38401 tatgggtggt tatgccaccg gtaacgccct cgcccatgcc ggcgttattg gcggtgcaga
    38461 tatgactgta gaagccacgc taaccaaact gcattacctg ctgagccagg aactggatac
    38521 tgaaaccatt cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc cggatgatta
    38581 aggagactgc aatgccccat cgcgccctgt tactggtcga tttacaaaat gatttctgtg
    38641 ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt agatgtcgct aaccgcctga
    38701 ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg cacccggcga
    38761 atcacggcag ttttgccagt cagcacggtg tagagcctta tacgccaggc caactcgacg
    38821 gtttgccaca aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc gcacaattac
    38881 atccgttact gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa aatcctttag
    38941 ttgacagtta cagtgccttt tttgataacg gccgtcggca gaaaacctct ctcgatgact
    39001 ggttacgcga tcatgaaatc gatgaattga tcgttatggg cctggctact gactattgcg
    39061 tgaagtttac cgtgctggac gcgttacagt taggttataa ggtaaacgtg attaccgatg
    39121 gttgtcgtgg cgtgaatatc cagccccagg acagtgcgca cgcgtttatg gagatgtcag
    39181 cagctggggc aacgctatat acgctggcag actgggaaga gacgcagggg taatattagt
    39241 tgaattcatc tctggcctac atttcctcaa tgacattgta ggccagataa tacttgtcag
    39301 tgttgcattt aacctacttg cagctaaaac acgacgtcct aacaagtata tttgccctcc
    39361 cttaataaca ggcttaaata ctagataact cattcactta aacatagcaa taggctttgc
    39421 tgagatacca aaatcttctt ttaactgctg tttgctcttc atgaccatct gaccatttgt
    39481 gtcaattgtc atatgctgtg cttcggtatt atggcgggct tgccacagca tcaccagttg
    39541 caggcaattc tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt tccccagttc
    39601 aacggcggtc gacaaacgct ggtatacctc aggcgtcatg ctgttgataa tgtcatcaag
    39661 attcataatt tttccgctcc tgtggaataa gttgctgaat cgttttttca accgttgatt
    39721 tcttcgccgt tttcgccatc agtgaagcgt aaagaagcag agttaacaca ataacgttcg
    39781 cctgttggtt gcggcccgtc gggaaagaca tgccccagat gggcatcaca gttaccgcaa
    39841 cgaatttcta tgcgctgcat tccatgtgac aagtctttga tataacgaat ggattcttca
    39901 ctcaccggtt cgtagaaact gggccagcca cagccggaat catacttggt ttcggaatga
    39961 aacagcgggg catcgcagat caaacagtga tatacgccgt cacgcttgtt atgcagtaaa
    40021 cgacccgtaa atggcggttc tgtcccatga ttctgcgtca cgtaaaactg catctcggac
    40081 aaattttttt tcagttcttc tgccgaaggt ttattagcca tttgctcaca tctcacttta
    40141 atcgtgctca cattacgtga ctgattctaa caaaacatta acaccaactg gcaaaatttt
    40201 gtcctaaact tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa tcacattttt
    40261 atcgtaattg ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag cccaaaggaa
    40321 gagtgaggcg agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg caatgattga
    40381 cacgattccg cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc ttttattcac
    40441 taacaaatag ctggtggaat atatgactat caaagtaggt atcaacggtt ttggccgtat
    40501 cggtcgcatt gttttccgtg ctgctcagaa acgttctgac atcgagatcg ttgcaatcaa
    40561 cgacctgtta gacgctgatt acatggcata catgctgaaa tatgactcca ctcacggccg
    40621 tttcgacggt accgttgaag tgaaagacgg tcatctgatc gttaacggta aaaaaatccg
    40681 tgttaccgct gaacgtgatc cggctaacct gaaatgggac gaagttggtg ttgacgttgt
    40741 cgctgaagca actggtctgt tcctgactga cgaaactgct cgtaaacaca tcaccgctgg
    40801 tgcgaagaaa gtggttatga ctggtccgtc taaagacaac actccgatgt tcgttaaagg
    40861 cgctaacttc gacaaatatg ctggccagga catcgtttcc aacgcttcct gcaccaccaa
    40921 ctgcctggct ccgctggcta aagttatcaa cgataacttc ggcatcatcg aaggtctgat
    40981 gaccaccgtt cacgctacta ccgctactca gaaaaccgtt gatggcccgt ctcacaaaga
    41041 ctggcgcggc ggccgcggcg cttcccagaa catcatcccg tcctctaccg gtgctgctaa
    41101 agctgtaggt aaagtactgc cagaactgaa tggcaaactg actggtatgg cgttccgcgt
    41161 tccgaccccg aacgtatccg tagttgacct gaccgttcgt ctggaaaaag ctgcaactta
    41221 cgagcagatc aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag gcgttctggg
    41281 ctacaccgaa gatgacgtag tatctaccga tttcaacggc gaagtttgca cttccgtgtt
    41341 cgatgctaaa gctggtatcg ctctgaacga caacttcgtg aaactggtat cttggtacga
    41401 caacgaaacc ggttactcca acaaagttct ggacctgatc gctcacatct ccaaataagt
    41461 tgagatgaca ctgtgatcta aaaagagcga cttcggtcgc tctttttttt acctgataaa
    41521 atgaagttaa aggactgcgt catgattaag aaaatttttg cccttccggt catcgaacaa
    41581 atctcccctg tcctctcccg tcgtaaactg gatgaactgg acctcattgt ggtcgatcat
    41641 ccccaggtaa aagcctcttt tgcattacag ggcgcacacc ttctctcgtg gaaacctgcg
    41701 ggtgaagaag aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg cgtcgctatt
    41761 cgcggtggcg taccggtttg ctggccgtgg tttggtccgg cggcacaaca aggtctgcct
    41821 gcgcacggtt ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga agatgctgat
    41881 ggcgtagcgc tgacttttga attgacgcaa agcgaagaga cgaaaaaatt ctggccgcac
    41941 gactttacgc tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga tcttgaatca
    42001 catggcgaat ttgaaaccac ctctgccctg catacctact ttaacgtggg tgatatcgct
    42061 aaggtaagcg tcagtgggct gggcgatcgc ttcattgata aagtaaatga cgcgaaagaa
    42121 gatgtactga ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt gtatctgaat
    42181 ccgcaagatt gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc cgtaggccac
    42241 cagcatcatc tgaacgttgt cggctggaac ccgggaccgg cgctttcagt tagcatgggc
    42301 gacatgccgg atgatggcta caaaacattt gtttgtgtag aaacggctta cgcttcagaa
    42361 acgcaaaaag tgaccaaaga gaaacctgca catctggcgc aatccattcg cgttgcgaaa
    42421 cgttaatttg cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta tcaacatcac
    42481 accatatcca gcgcagtttt tccttttggt gccggatacg ctttatccag catggttaat
    42541 tccgctgacg aaagttcgac ctcaagcaca gccgcattct gttggacatg ggcaatcgtg
    42601 gccgcttttg gaatcgccat cacaccctga tggctgatta cccacgccaa caatacttgt
    42661 gccgcgctga tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt taacagtcca
    42721 ttgcgcaacc gcccggcctg ggctaatgga ctgtaagcca tcaccggcat ctgctgttgc
    42781 tggcaccagg ggagtagatc gtactcaatc cctcgtgaac cgagatggta aagcacctga
    42841 ttagtggcac actgatttcc ccccggcagc tgccagagtt cctgcatatc agcatgatca
    42901 aggttagaaa cgccccagcg gcggattttt ccctgggcga tcaatttttc catcgctgcc
    42961 acagtctctt caaaagcgaa actgccagac cagtgtaata agtagagatc gagataatca
    43021 gtattgagac ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc gccagcattc
    43081 cacggataga ctttagagac gagaaagacg ttatcgcgca gaccggttaa tgcttcccca
    43141 accacctttt cggcaccgcc atcggcatac atttcggcgg tatcaatgag ggttaaaccg
    43201 agctcaatgc ccgcgcgtag tgcagcaact tctgttttgc gctgactggc atcttcgccc
    43261 atataccatg ttccctgccc tatggctggc agtgagacat cgccactaaa ttgaatcatt
    43321 ttttgttgca ttgtttcctc ccaggtattg caccaccgta atgcaaaaca gggcgtgacg
    43381 ccctgttttt atgcacaaaa tgccctggaa agatgcatta tcagaatttg taggtgatcc
    43441 cggtagaaat caggccagtc caggatttat ccaccatcgg gctgtcagta acttcatcag
    43501 acagacgggt atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag ttgtagctgg
    43561 cgctcagctc caggtaaggg ctccagctgt cgttcgggtt atagccacgc agaccgctgc
    43621 gagcggactc tttgcgcgat acgccataat agtattcgtt ctggttttcg ctgttccact
    43681 gcacaccgat acccggggtc acggtcaggc cgccgttggt gtaacgatac aaccaggcca
    43741 tatcccagac gatgccgttg ctgttatcca gggtatcgcc agccagggtg gtacgcaggt
    43801 aaccgtactg ggtaaagtga gcataagaca gaccagccat catggtgctc ttacggtcat
    43861 ccaggtgacg catttggtga tcgccactgt ctttcgcttt gaagtaaagc ggcgaccaat
    43921 aagcggtaat tgaaagtttg tccgttgcgt cattccacag gtagtaacca ccacctaagc
    43981 cacggaacca gaagttatcg ccttcatagt tgattaccgg tactgggtaa acatcggtat
    44041 cgtaatcttt atatgggtgc tcaacgacac ctacgcctgc gcccagggaa aatttacctt
    44101 cagcgtgcgc tacgcctgca gacgttgcga taagcactcc aagtgccaga agtttgagtt
    44161 tggtcacaat taatcattcc ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa
    44221 tacatcgaaa cccaaccttt ttacgttttc attttttaaa gtaactgttt aattttcctg
    44281 acgcggatga caccgcgctt acagccaaat gaatttagcg ttactggcta ccctggtctt
    44341 tacattaatt atgcaaaatt tatggatgag ttgttgatat gccattgaaa ttaagaaagc
    44401 cgtgcaggca agttttccat ttgccatcta cgcttaattt tgaaggtgta tcaccgggca
    44461 cgttgttctc atcgtcgata aaatggcatg agagttgctg tgttttagca agagacgtcg
    44521 ttcagtttac ctcttccggg agcctctact attcatatga acggctctta acctgtgcta
    44581 aaaaacgaaa ggacggcata ccatgaatat attcgatcac tatcgccagc gatatgaagc
    44641 tgccaaggac gaagagttca cactgcagga gtttcttacc acttgtcggc aagatcgcag
    44701 tgcttatgcc aacgcggctg agcggctatt gatggctatc ggtgagcctg tcatggtcga
    44761 tacagcccag gaacccagac tttctcgact cttttctaac cgggtcattg cacgttatcc
    44821 ggcatttgaa gagttttacg gcatggaaga cgcgattgaa cagattgtct cttatctgaa
    44881 acacgcggct caggggctgg aagagaagaa acaaatcctg tatctgctgg ggcctgtggg
    44941 tgggggtaaa tcatcgcttg ctgagcgact gaaatcatta atgcagctcg taccgattta
    45001 tgtattgagc gccaacggtg agcgtagccc ggtcaacgat catccgttct gtcttttcaa
    45061 tccgcaggaa gatgctcaga ttctggaaaa agagtatggc attcctcgcc gttatctcgg
    45121 caccatcatg tcgccatggg cggcaaaacg cctgcatgaa tttggtggcg atatcactaa
    45181 gttccgggta gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg ccaaaacgga
    45241 acccggtgat gagaacaacc aggacatctc cgcgctggtt gggaaagtcg atattcgtaa
    45301 actcgaacac tacgcgcaga atgacccgga cgcctacggc tattccggtg cgctgtgccg
    45361 cgccaaccaa gggatcatgg aatttgtcga gatgtttaaa gcaccgataa aagtgctgca
    45421 tccgctgtta accgccactc aggaaggtaa ctacaacggg acggaaggta tctccgccct
    45481 gccgttcaac gggattattc tcgcccactc gaacgaatcc gaatgggtca ctttccgtaa
    45541 taacaaaaac aacgaagcct tcctcgaccg tgtttacatc gtgaaggtgc cgtattgctt
    45601 acgcatttcc gaagagatca aaatctacga gaaattgctt aatcacagtg aattgactca
    45661 cgccccctgc gcccctggca cgctggaaac gctgtcacgt ttttccattc tttcgcgcct
    45721 taaagagcca gaaaactcca gcatttattc aaagatgcgg gtttatgatg gcgaaagtct
    45781 gaaagacacc gatcccaaag ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga
    45841 tgaagggatg aacggtctgt cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa
    45901 cttcgatcat gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc tggaacagca
    45961 gatcgagcgc gagcagttcc cacaagagca ggcagaacgc tatctggagt tcctgaaagg
    46021 ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg cctaccttga
    46081 atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg cggatttctg
    46141 gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc gcgagtctct
    46201 taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa aagatttccg
    46261 caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac gcaatccgaa
    46321 ctggaccagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct ccaataccga
    46381 ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc agaagaaaca
    46441 cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg tgcgtttact
    46501 gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg gtacacatgt
    46561 taccgggcct acaacgacag cgaaccgtgg gcctgagaag cggcaacaca ggcgtagcat
    46621 acagttggca aatgtagtac ggggggcata tgacctggtt tattgaccgg cgtctgaacg
    46681 gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa gcgcaaatta
    46741 aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac agcggcgaat
    46801 ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg cgtggcggtc
    46861 tgcgccaccg cgtgcatccg ggcaatgacc atttcgtcca gaacgaccga attgaacgtc
    46921 cccagggtgg cggcggaggt tccggtagtg gtcagggcca ggccagccag gatggtgaag
    46981 gtcaggatga atttgtcttt cagatttcga aagatgagta tcttgatttg ctctttgaag
    47041 atttggcctt accgaatctg aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc
    47101 atcgggcggg ttataccgct aacggcgttc cggcaaatat cagcgttgtg cgctccttgc
    47161 agaactcact ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa cttcatgcac
    47221 tggaagagaa tttggccatc atcagcaaca gtgaacctgc gcagctgctg gaagaggaac
    47281 gtctgcgcaa agaaattgca gaattacgtg ccaaaattga acgcgtccct tttattgaca
    47341 ccttcgattt acgttacaag aactacgaga agcggcccga tccctccagc caggcagtga
    47401 tgttttgcct gatggacgtt tccggttcaa tggatcaatc cactaaagat atggctaagc
    47461 gtttttatat tctgctgtat ctgttcctca gcagaacgta taagaacgtg gaagtcgtat
    47521 acatccgcca tcatacccag gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg
    47581 aaacaggcgg caccattgtt tccagcgccc tgaaactgat ggatgaggta gtgaaagagc
    47641 gttataaccc ggcacagtgg aatatttacg ctgcacaagc atcggacggc gataactggg
    47701 ccgatgactc tccgctttgc catgaaatcc tggcgaaaaa attattacct gttgttcgtt
    47761 attacagcta tatcgaaatt acccgtcgtg cacatcagac attgtggcga gaatatgagc
    47821 atctgcaatc tactttcgac aactttgcga tgcagcacat ccgcgaccag gatgatattt
    47881 atccggtgtt ccgtgaactg tttcataaac aaaatgcaac agctaaagac taaaactatc
    47941 agccaggtca ttatcgcctg gctgattttt agcttactgt aaattatctc atttattaca
    48001 tacattagct tacaatcgct ttaaatatga cagcgtaacc tttacataat ttagttccag
    48061 aaaacaatca ttcggaaaaa tgattcagtc aa
//
LOCUS       DAZFDP010000018        45703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFDP010000018 DAZFDP010000000
VERSION     DAZFDP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            214..840
                     /gene="ydhY"
                     /locus_tag="ACHJ1G_001146"
     CDS             214..840
                     /gene="ydhY"
                     /locus_tag="ACHJ1G_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4763039.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            861..2963
                     /gene="ydhV"
                     /locus_tag="ACHJ1G_001147"
     CDS             861..2963
                     /gene="ydhV"
                     /locus_tag="ACHJ1G_001147"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4763040.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2976..3614
                     /gene="ydhW"
                     /locus_tag="ACHJ1G_001148"
     CDS             2976..3614
                     /gene="ydhW"
                     /locus_tag="ACHJ1G_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4763041.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3678..4346
                     /gene="ydhX"
                     /locus_tag="ACHJ1G_001149"
     CDS             3678..4346
                     /gene="ydhX"
                     /locus_tag="ACHJ1G_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4763042.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4343..5128
                     /gene="phsC"
                     /locus_tag="ACHJ1G_001150"
     CDS             4343..5128
                     /gene="phsC"
                     /locus_tag="ACHJ1G_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4763043.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5132..5944
                     /gene="ydhT"
                     /locus_tag="ACHJ1G_001151"
     CDS             5132..5944
                     /gene="ydhT"
                     /locus_tag="ACHJ1G_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4763044.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5956..7560)
                     /gene="ydhS"
                     /locus_tag="ACHJ1G_001152"
     CDS             complement(5956..7560)
                     /gene="ydhS"
                     /locus_tag="ACHJ1G_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4763045.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7686..7991)
                     /gene="ydhR"
                     /locus_tag="ACHJ1G_001153"
     CDS             complement(7686..7991)
                     /gene="ydhR"
                     /locus_tag="ACHJ1G_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4763046.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8099..8175)
                     /locus_tag="ACHJ1G_001154"
     tRNA            complement(8099..8175)
                     /locus_tag="ACHJ1G_001154"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8139..8141),aa:Val,seq:gac)
     gene            complement(8180..8256)
                     /locus_tag="ACHJ1G_001155"
     tRNA            complement(8180..8256)
                     /locus_tag="ACHJ1G_001155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8220..8222),aa:Val,seq:gac)
     gene            8564..9820
                     /gene="ydhQ"
                     /locus_tag="ACHJ1G_001156"
     CDS             8564..9820
                     /gene="ydhQ"
                     /locus_tag="ACHJ1G_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4763047.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9861..11234)
                     /gene="mdtK"
                     /locus_tag="ACHJ1G_001157"
     CDS             complement(9861..11234)
                     /gene="mdtK"
                     /locus_tag="ACHJ1G_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4763048.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11449..12090
                     /gene="ribE"
                     /locus_tag="ACHJ1G_001158"
     CDS             11449..12090
                     /gene="ribE"
                     /locus_tag="ACHJ1G_001158"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4763049.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12130..13278)
                     /gene="cfa"
                     /locus_tag="ACHJ1G_001159"
     CDS             complement(12130..13278)
                     /gene="cfa"
                     /locus_tag="ACHJ1G_001159"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4763050.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13569..14780)
                     /gene="punC"
                     /locus_tag="ACHJ1G_001160"
     CDS             complement(13569..14780)
                     /gene="punC"
                     /locus_tag="ACHJ1G_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4763051.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14893..15825
                     /gene="punR"
                     /locus_tag="ACHJ1G_001161"
     CDS             14893..15825
                     /gene="punR"
                     /locus_tag="ACHJ1G_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4763052.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15822..16847)
                     /gene="purR"
                     /locus_tag="ACHJ1G_001162"
     CDS             complement(15822..16847)
                     /gene="purR"
                     /locus_tag="ACHJ1G_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4763053.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17146..17235
                     /gene="cydH"
                     /locus_tag="ACHJ1G_001163"
                     /gene_synonym="ynhF"
     CDS             17146..17235
                     /gene="cydH"
                     /locus_tag="ACHJ1G_001163"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4763054.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17401..18570
                     /gene="ydhP"
                     /locus_tag="ACHJ1G_001164"
     CDS             17401..18570
                     /gene="ydhP"
                     /locus_tag="ACHJ1G_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763055.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18716..19297)
                     /gene="sodB"
                     /locus_tag="ACHJ1G_001165"
     CDS             complement(18716..19297)
                     /gene="sodB"
                     /locus_tag="ACHJ1G_001165"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4763056.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19425..20252)
                     /gene="mepH"
                     /locus_tag="ACHJ1G_001166"
     CDS             complement(19425..20252)
                     /gene="mepH"
                     /locus_tag="ACHJ1G_001166"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4763057.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20587..20934
                     /gene="grxD"
                     /locus_tag="ACHJ1G_001167"
     CDS             20587..20934
                     /gene="grxD"
                     /locus_tag="ACHJ1G_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4763058.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20985..25601)
                     /gene="lhr"
                     /locus_tag="ACHJ1G_001168"
     CDS             complement(20985..25601)
                     /gene="lhr"
                     /locus_tag="ACHJ1G_001168"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4763059.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPDGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25694..26341)
                     /gene="rnt"
                     /locus_tag="ACHJ1G_001169"
     CDS             complement(25694..26341)
                     /gene="rnt"
                     /locus_tag="ACHJ1G_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4763060.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26444..26851)
                     /gene="gloA"
                     /locus_tag="ACHJ1G_001170"
     CDS             complement(26444..26851)
                     /gene="gloA"
                     /locus_tag="ACHJ1G_001170"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4763061.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26932..28029)
                     /gene="nemA"
                     /locus_tag="ACHJ1G_001171"
     CDS             complement(26932..28029)
                     /gene="nemA"
                     /locus_tag="ACHJ1G_001171"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4763062.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28066..28665)
                     /gene="nemR"
                     /locus_tag="ACHJ1G_001172"
     CDS             complement(28066..28665)
                     /gene="nemR"
                     /locus_tag="ACHJ1G_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4763063.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28768..29007
                     /gene="ydhL"
                     /locus_tag="ACHJ1G_001173"
     CDS             28768..29007
                     /gene="ydhL"
                     /locus_tag="ACHJ1G_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4763064.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29056..29952
                     /gene="ydhF"
                     /locus_tag="ACHJ1G_001174"
     CDS             29056..29952
                     /gene="ydhF"
                     /locus_tag="ACHJ1G_001174"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO4763065.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30033..30554
                     /gene="sodC"
                     /locus_tag="ACHJ1G_001175"
     CDS             30033..30554
                     /gene="sodC"
                     /locus_tag="ACHJ1G_001175"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4763066.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30555..32567)
                     /gene="ydhK"
                     /locus_tag="ACHJ1G_001176"
     CDS             complement(30555..32567)
                     /gene="ydhK"
                     /locus_tag="ACHJ1G_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4763067.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32567..33424)
                     /gene="ydhJ"
                     /locus_tag="ACHJ1G_001177"
     CDS             complement(32567..33424)
                     /gene="ydhJ"
                     /locus_tag="ACHJ1G_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4763068.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33427..33663)
                     /gene="ydhI"
                     /locus_tag="ACHJ1G_001178"
     CDS             complement(33427..33663)
                     /gene="ydhI"
                     /locus_tag="ACHJ1G_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4763069.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33864..34298
                     /gene="slyA"
                     /locus_tag="ACHJ1G_001179"
     CDS             33864..34298
                     /gene="slyA"
                     /locus_tag="ACHJ1G_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4763070.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34345..34812)
                     /gene="slyB"
                     /locus_tag="ACHJ1G_001180"
     CDS             complement(34345..34812)
                     /gene="slyB"
                     /locus_tag="ACHJ1G_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4763071.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35086..36195
                     /gene="anmK"
                     /locus_tag="ACHJ1G_001181"
     CDS             35086..36195
                     /gene="anmK"
                     /locus_tag="ACHJ1G_001181"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4763072.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36293..36622
                     /gene="mliC"
                     /locus_tag="ACHJ1G_001182"
     CDS             36293..36622
                     /gene="mliC"
                     /locus_tag="ACHJ1G_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4763073.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36681..37337
                     /gene="pdxH"
                     /locus_tag="ACHJ1G_001183"
     CDS             36681..37337
                     /gene="pdxH"
                     /locus_tag="ACHJ1G_001183"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4763074.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37466..38740
                     /gene="tyrS"
                     /locus_tag="ACHJ1G_001184"
     CDS             37466..38740
                     /gene="tyrS"
                     /locus_tag="ACHJ1G_001184"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4763075.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38799..39662
                     /gene="pdxY"
                     /locus_tag="ACHJ1G_001185"
     CDS             38799..39662
                     /gene="pdxY"
                     /locus_tag="ACHJ1G_001185"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO4763076.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39706..40311)
                     /gene="gstA"
                     /locus_tag="ACHJ1G_001186"
     CDS             complement(39706..40311)
                     /gene="gstA"
                     /locus_tag="ACHJ1G_001186"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4763077.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40417..41919)
                     /gene="dtpA"
                     /locus_tag="ACHJ1G_001187"
     CDS             complement(40417..41919)
                     /gene="dtpA"
                     /locus_tag="ACHJ1G_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4763078.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42530..43165)
                     /gene="nth"
                     /locus_tag="ACHJ1G_001188"
     CDS             complement(42530..43165)
                     /gene="nth"
                     /locus_tag="ACHJ1G_001188"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4763079.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43165..43860)
                     /gene="rsxE"
                     /locus_tag="ACHJ1G_001189"
     CDS             complement(43165..43860)
                     /gene="rsxE"
                     /locus_tag="ACHJ1G_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4763080.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43864..44484)
                     /gene="rsxG"
                     /locus_tag="ACHJ1G_001190"
     CDS             complement(43864..44484)
                     /gene="rsxG"
                     /locus_tag="ACHJ1G_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4763081.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44488..45546)
                     /gene="rsxD"
                     /locus_tag="ACHJ1G_001191"
     CDS             complement(44488..45546)
                     /gene="rsxD"
                     /locus_tag="ACHJ1G_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4763082.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45547..>45703)
                     /locus_tag="ACHJ1G_001192"
     CDS             complement(45547..>45703)
                     /locus_tag="ACHJ1G_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFO4763083.1"
                     /translation="AIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQ
                     QKVVNED"
BASE COUNT        11522 a        11298 c        11914 g        10969 t
ORIGIN      
        1 gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct aaccataaag
       61 gattagttta tcggcgttaa atcttttctc atacactatc gggttaggga aaatatctgg
      121 tgttggggac agagtcagca gcgtattagt tgaatgctta atatcaggct gctgaattgt
      181 cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta
      241 gatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggtcta
      301 acaattgccc ctgcgttact gtcacttttg ggttgcaaac aggaagatat tgatagcggc
      361 acagtagggt tgataaatac acccaaaggg gtgctggtga cccagcgagc gcgatgcacg
      421 ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc
      481 ttctcccgta tcaaaatcca ccgcaattat ttctttggcg acaacggggt tggctctggc
      541 ggcggcctgt atggcgatct caactatacc gcggacacct gccgtcaatg caaagagccg
      601 caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt
      661 accgtcgatc ataaacgctg tattggctgt agcgcctgta ctacggcatg cccgtggatg
      721 atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc
      781 gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga
      841 atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc
      901 tcacgacagg aaatattacc ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca
      961 tgggcttcgg ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg
     1021 aagcgaataa attagtcttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt
     1081 ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtt gtcgatgccc
     1141 atatgggtgg cttttttgca gcgcaaatga aattcgctgg atacgacgtc attattatcg
     1201 aagggaaggc gaaatcaccg gtatggctga agattaaaga tgacaaagtt agcctggaaa
     1261 aagccgattt cttatgggga aaagggacgc gcgcaacgac ggaagaaatt tgtcgattga
     1321 ccagtccgga aacctgtgtg gcggctattg gtcaggctgg ggaaaacctt gttcctctct
     1381 ctggcatgtt gaatagccgt aaccacagcg gcggtgcggg aactggcgca attatgggtt
     1441 cgaaaaacct gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc
     1501 aggagatgaa gcgtctcaac gattacatga tgactgaact tattggtgcg aataacaacc
     1561 atgtcgtgcc aagtacgccg caatcgtggg cagagtattc agatcccaag tcacgttgga
     1621 cagcacgtaa agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa acgggggaaa
     1681 ttccgccagg caatcagaac acggtcggct ttcgtaccta taaatccgtt tttgacttag
     1741 gtccggcggc agagaaatac acagtaaaaa tgagtggctg ccactcttgc ccgatccgtt
     1801 gtatgaccca aatgaatatt cctcgggtga aagagtttgg cgtgcccagc acaggtggta
     1861 acacttgtgt agctaacttt gtccatacca ccatcttccc gaacgggccg aaagattttg
     1921 aagataaaga cgatggtcgt gtgattggta acctggttgg tctgaatttg ttcgatgact
     1981 acggcctatg gtgtaactac gggcagctgc atcgcgactt tacttattgt tacagcaaag
     2041 gggtgttcaa gcgtgttctg ccagctgaag agtatgcgga aattcactgg gatcaactgg
     2101 aagcgggtga cgttaatttc attaaagatt tttactaccg tctggcgcat cgtgtgggtg
     2161 agctgagtca cctggctgat ggttcatatg ccatcgcaga acgctggaat ttgggtgaag
     2221 agtactgggg ctacgcaaaa aataaactct ggtcgccgtt tggctatccg gttcaccatg
     2281 ccaatgaagc gtcagcgcag gtcggttcca ttgttaactg tatgttcaac cgtgactgca
     2341 tgacgcatac ccatatcaat tttattggtt cgggtttgcc attgaaactg cagcgtgaag
     2401 tggcgaaaga gctttttggt tctgaagatg cctacgatga aaccaaaaac tacacgccaa
     2461 tcaatgacgc aaaaattaaa tatgcaaagt ggtcgctgtt gcgggtctgt ttgcataacg
     2521 ccgtcaccct gtgcaactgg gtctggccaa tgaccgtttc gccgctgaaa agccgtaatt
     2581 accggggcga tctggcgctt gaagccaaat tcttcaaagc gatcaccggc gaagagatga
     2641 ctcaggaaaa attagattta gctgcagagc gtatctttac gttgcatcgt gcctacacgg
     2701 taaaactgat gcaaaccaaa gatatgcgta atgaacacga tcttatctgt tcctgggtat
     2761 tcgacaagga tccgcagatc ccggtcttta ctgaaggtac tgacaaaatg gatcgtgacg
     2821 atatgcatgc ttcgctcacg atgttctaca aagaaatggg ctgggaccca cagcttggtt
     2881 gtccgacccg cgaaacattg cagcgtctgg ggctggaaga tattgccgcc gatctggcag
     2941 cacacaatct actgcctgtg taaggaatgg gtgagatgaa ccatcgggat gaattgccgt
     3001 tagcgaaagt cagcgaagtt gacgaggcga agcggcaatg gttacagggg atgcgtcacc
     3061 ccgttgatac ggtaaccgaa ccagaaccgg ctgaaatcct ggcagagttc attcgccagc
     3121 attctgcggc tgggcagctg gtggcgcgcg ccgtgtttct ctcgccaccc tattcggttg
     3181 cagaagagga gctgtcagtt ttgctggaaa gcattaagca gaacggagac tacgcggata
     3241 tcgcttgtat gacgggaagc caggatgatt actattactc aacgcaggcc atgagtgaaa
     3301 actacgcggc gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc attgcacacg
     3361 ccgttcgttt tgagtgccag acatatcctc gtccttacaa agtcgccatg ctgatgcaag
     3421 cgccatatta cttccaggaa gcgcagattg aagccgcgat cgccgcgatg gatgttgcac
     3481 ctgaatatgc cgatatacgg caggtggagt catcgacggc ggtgttgtac ttgtttagcg
     3541 agcgatttat gacctatggc aaggcttatg gcttatgcga atggttcgag gtagagcagt
     3601 ttcagaaccc ctgaaatttc agggagatgt catggatagg gtggacagtt gcagccactg
     3661 cattagggga taatcagatg tccttcactc gacgcaaatt tgttctgggg atgggaacag
     3721 taatcttttt tactggttct gcgtcttctc tgttagcgaa cacgaggcaa gaaaaggaag
     3781 ttcggtatgc catgattcat gacgagtcac ggtgcaatgg ctgtaatatt tgcgcccgcg
     3841 catgccgtaa aactaaccac gtccccgctc agggaagccg tttatcgata gcacacattc
     3901 cagtcaccga taacgacaac gaaacgcagt atcacttttt tcgccagtca tgtcagcact
     3961 gcgaagatgc tccgtgcatc gacgtatgcc cgacaggggc gtcgtggcgt gatgagcagg
     4021 ggatcgtgcg ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt ggcgcttgtc
     4081 cgtaccaggt tcgctacctt aaccctgtca ctaaagtggc agataaatgt gatttctgcg
     4141 ctgagtcccg actggcaaaa ggctttccgc ccatttgcgt cagcgcctgc ccggaacacg
     4201 cattgatctt tggtcgcgaa gatagcccag aaattcaggc gtggttgcag caaaataaat
     4261 actatcaata tcagctacct ggtgccggga aaccgcatct gtatcgccgg ttcggtcaac
     4321 atttgattaa aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt ttcagagcca
     4381 gttagcgaac tatgtgccac agttcactcc cgaattttgg ccggtgtggt tgatcattgc
     4441 cggagtattg ctggttggga tgtggttggt gctggggctg catgccttgc ttcgtgctcg
     4501 tggcgtgaag aaatcagtca ccgattatgg tgagaagatt tatctttact gcaaagcggt
     4561 cagattatgg cactggtcga atgcgttact ctttgtattg ttgctggcaa gtgggctgat
     4621 aaatcacttt gcgctggtgg gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt
     4681 ttgcggattt ttgttactcg catgctggct cggctttgtg ctgatcaatg ccgttggggg
     4741 taatggtcac cactatcgca ttcgtcgtca ggggtggctg gagcgagcgg caaaacaaac
     4801 gcgattttat ttgtttggca ttatgcaggg ggaagaacat cctttcccgg caacaaccca
     4861 gtctaaattt aatcccttac agcaggtcgc ctatgttggt gttatgtatg gattgctgcc
     4921 gttgttacta ttgacggggc tgctgtgtct ctatccgcaa gccgtgggag atgtgtttcc
     4981 tggcgtaaga tactggttat tgcaggcgca ttttgctctg gcatttataa gcctcttttt
     5041 tatcttcggt catctttatc tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag
     5101 catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag acctgcgaga
     5161 atggcgtatc ggagcggtga tgtatcgctg gttcctgcgc catttcccac gcggcggtag
     5221 ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg cggagagtct
     5281 ggttgagtat gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc aggaagtttc
     5341 ctctatgcaa aaactggcca cagaccccgg agagatacca ttttgcagtc agtttgcccg
     5401 cagcgacgat catgctcgca tcggctgttg tgaggataac gcccgaatcg caaccgctgg
     5461 gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg tcggctttaa
     5521 cagtcacatt ggcagctccg gtgaacgcgc acgggttgct gtaaccggta attcttcgcg
     5581 gataagcagc gctggagata gtagtcggat tgcgaatacc ggaatgcgag tacgcgtttg
     5641 tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa tcgccagctt
     5701 tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg ccagcggtga
     5761 aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc cagggggaag
     5821 cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga
     5881 aaacaatatt cgaaccggag tccgctatcg tcttaatgaa cagcatcagt ttgttgagtg
     5941 ctgatagctt tacctttaat caaacgaaag acgccgacga gcacgggatg caggcttcac
     6001 gaccgcccga gccatcgcct caccaatttc tgcacatgcc gtaagtccct gaacgaaagg
     6061 ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa tatcttcggg
     6121 ttgctgtaac gtataatctt cgccaatatc agggatttca tcccctgttt tctgtaattg
     6181 ctcgcgtagc ccagggaaag gaatatcttt cactttaagc ggacgctgtc cgcgggcatc
     6241 aataaaaacg tcaaacgagt agctgttgtc ttccgttttc aggacggtgc gcgactcatt
     6301 aatttccatt ttgtagtctt caccgagggc gagaatatga atgattcccg cttcacgtaa
     6361 ggcaagtagg cgacgaatag actctgaagg gattgccgca taattatcga tgaaaaccct
     6421 ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgaggaa caatttcctg
     6481 tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt tttctcgttt
     6541 attgcgttcg acttcctgga gatttttttc tgcccagtca aatgggtcgc gttgtttgcg
     6601 ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg ctatgcgttg
     6661 gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata cccgatccaa
     6721 aaggccctct tcaccttttt gaatctcagc atttaatgcc tgatcggtga cgatgtgtaa
     6781 gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacccg tgcgcgacat
     6841 caacgtaata tttagctttt cacttgcgtt atcgcggtta aaaacgacgt gttgtttatc
     6901 atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca gtccgctcaa
     6961 ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca ggcctgacca
     7021 cggactggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt gacccgtggc
     7081 aatcaccgct aaatcaaacg tctcgctggg taaatcctga tttgtagcga gcatgacgcc
     7141 agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca ctgcaaattt
     7201 ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag
     7261 aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat aacgctggag
     7321 atggctggct tcttgctttt gtagccattc gagatacgta caattaatcg gcggtatttc
     7381 aatactggca atatttgcca gcatcatttt tgagttttcc tcatcactgt atggcatccc
     7441 gacaccggcc tcgtcagcct gctcgaagat agaaatagaa agtggagttt gttgctgtag
     7501 aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa tttttttcat
     7561 tgttacttac ctgtacgcat tctttcgaat ttagtgttat cagataagtt tggttgagat
     7621 ttttagaatt tgagaaattt cagacaggta tgaaaagggc gctaaccgcc cttgattaaa
     7681 ttctgtcagg cgagttttgc ctgattgatt tgactcagtg gctcattgac atcgaaaact
     7741 ttggcgacaa cttcctcaac gccgagattt ttaagtcggg cagtatgttt ttccagatag
     7801 gcaagggcgc ttttttcatc agtaaaaaga tagatcccac cggcttcgtg gttcttttca
     7861 ctttctgtcc ataccttcca cagaaaacca ggttcctgat taatcgactc agcaagtggt
     7921 ttaagctgct cagccattgc gtcgccaaat gggccattaa aagcaaaatg aagttgtaac
     7981 aaagttgcca tagcaattct ccttctgata acgcatacga agacagtgta aatcttttaa
     8041 cgtattggat acagagaaga aacagaggaa aaaaggagaa gaccagatta agaaaatctg
     8101 gtgcgtccga gtggactcga accaccgacc cccaccatgt caaggtggtg ctctaaccaa
     8161 ctgagctacg gacgcaggat ggtgcgttca attggactcg aaccaacgac ccccaccatg
     8221 tcaaggtggt gctctaacca actgagctat gaacgcaacg ttgtaggtga caacggggac
     8281 gaatattagc ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc tgcgctattt
     8341 cgaccgtttg cagagctttt aagcaaattg gctatatttt gttgatttgc aagggcgatt
     8401 tttattcagg atcgcattta catctgatac aacccaataa ttcgtgtatc agagtttaaa
     8461 cagagttgtt tgccaccatc tcaaatctgt cgtggataaa tcctccgtag cctttacact
     8521 taggtagcaa taaaggcaca cagatgcagc aaaggaataa atcatgggat ctgatgcgaa
     8581 aaacttgatg agcgacggga atgtgcaaat tgttaagacc ggcgaggtca ttggcgcgac
     8641 gcaacttact gaaggcgagt tgattgttga agctggcgga agagccgaaa ataccgtggt
     8701 cacgggggct ggctggttga aagtggcaac cggtgggatc gccaaatgca cacagtacgg
     8761 taacaatggc acgctatcgg tcagcgacgg tgccattgcc acagatattg ttcagtccga
     8821 gggaggcgca attagtctct ctacgctcgc tacggttaat ggccgccatc ccgaaggtga
     8881 attcagcgtt gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg gtaacctgcg
     8941 tgtactggaa ggacatcgcg cggaaaaaat cattctcgat caagagggcg gcctgttggt
     9001 caatgggaca acctcagctg tcgtggtaga tgaaggtggt gaattgttgg tgtatccagg
     9061 tggggaagcc agcaattgtg aaattaatca gggcggcgtt tttatgctgg ccgggaaagc
     9121 cagtgatacg ttgcttgctg gtggcaccat gaataatctc ggtggtgaag actctgacac
     9181 tattgttgag aatggatcca tttatcgtct ggggacggat ggccttcagc tctacagttc
     9241 cggtaagacg caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc atgccggtac
     9301 gctggaaaat gcggtaatac aaggtggaac agtgatcctg ttgtcaccca ccagcgcgga
     9361 cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa ctgaccggga gtgttgcatt
     9421 actggacggc gcttcaatga ttattggcta tggtgcagat ctgcaacaat caacgattac
     9481 tgtacagcag ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg acagtgtcac
     9541 ttttagtgtt ggtaacatca atctgaatgg cggaaaactg tggctgatca ctggtgcggc
     9601 aacgcatgtg caactgaaag tgaaacgcct gcgcggagag ggagcgattt gcctgcaaac
     9661 cagtgcgaaa gaaatctcac ctgacttcat caatgtgaaa ggggaagtta ccggggatat
     9721 acgcgttgag ataacagatg ccagtcggca aactctgtgt aacgcactga aactacagcc
     9781 agacgaagac gggattggcg caacgctcca gcctgcgtaa aaacacaggc gcaacccgtg
     9841 aaggttgcgc cttgtcttta ttagcgggac gctcgttgca gaatgatggc tgacggcaga
     9901 cgttgcagga accgcatacg cagcatcatc ataatggctg ccgacgtcag gccaataata
     9961 aagcctatcc agaagcctgc tggccccata ggttcaacga ccagatcggt cagtgccaga
    10021 atatagccgc ttggcaagcc cagcacccag taagccgtaa aggtaatata gaaaatggaa
    10081 cgcgtatctt tataaccacg caaaatccca ctgccaatca cctggatcga gtcagaaatc
    10141 tgatataccg ccgccagcaa catcaaatgc gcagccagcg ttacaacctc gggattgtcg
    10201 ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga atatggccgt cagggttgcc
    10261 atacagacac ccaccataag cccggtccgc gcagcggttt gcgcatccag cgttgagccc
    10321 tgacccagac gataacctac gcggatagtt accgctgccg ccagcgacat gggaagcacg
    10381 aacattagtg aactaaagtt cagggcaatc tggtgtcctg cgacatcaac aataccgagc
    10441 ggagacacta acagagccac gacagcaaac agtgtcactt caaagaacag tgccagcgca
    10501 atcggcaaac cgagttgaat cagtcgtttc ataaccgcag gatcgggttt cgcggtgcct
    10561 ttttcgttac gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac catggcaagg
    10621 aacataaccc agtacaccgc cgcagtagcc acgccacaac caacgccacc gagctcaggc
    10681 ataccgaaat gaccataaat aaagatatag ttcaccggaa tgttcaccag caggccgata
    10741 aagcccatta ccatacccgg cttggttttt gccagacctt cacactggtt acgggcaacc
    10801 tggaagaaca gatagcccgg cgcgccccac aacaacgcac gcagataacc cacggctttg
    10861 tccgccagag ccggatcgat gttttccatg gagcggataa tgtaacctgc attccacagc
    10921 accagcataa tgagaacgga aacaaaacct gccagccaga aaccttgtcg cacctgatgc
    10981 gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga taaccggcgt taatgccagc
    11041 agcagtccgt gaccaaagag gatcgccgga agccagatag aagtaccgat agcgaccgcc
    11101 gccatgtcgg tggcactata gccgcccgcc atcacggtat cgacaaaacc catcgcagtt
    11161 tgggcgattt gcgcgagaat caccgggatt gctaatgcta ataacagacg cgcttcactg
    11221 atatacttct gcacgtgaac accttttatt tgtagttata tgagagacta aaaagccgcc
    11281 tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg aggatttatt
    11341 aattctccag tgaaaaaata gtcattctta ccccttcact ggtaagggcg tttgccacct
    11401 gctattgtgc tgaacagaat gtcttaactg atttcaggag ttgtaagtat gtttacgggg
    11461 attgtacagg gcaccgcaaa actggtgtcg attgacgaga aaccaaattt tcgtacgcat
    11521 gtggtggagt tacccgacca catgctggac ggcctggaaa ccggtgcgtc cgtggcgcat
    11581 aacggttgct gcctgaccgt gacggaaatt aacggcaacc atgtcagttt tgacctgatg
    11641 aaagaaacgt tacgcattac caatcttggc gatttaaaag tgggggattg ggtaaacgtt
    11701 gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact taatgtcagg tcatattatg
    11761 accactgctg aagtggcgaa aatattaacc tcagaaaata atcgccagat ctggtttaaa
    11821 gtccaggata gtcagttgat gaaatatatt ctgtacaaag gatttattgg catcgacggt
    11881 attagcctga ccgtcggcga agtcacgcca acgcgttttt gcgtccattt aattccggaa
    11941 acactggaac gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa cattgaaatt
    12001 gatccacaaa ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc acgagaaaat
    12061 gccatgaatc aaccaggtac agaagcctga tctgaactcg cccccgaaga gggggcgata
    12121 gaatagcctt tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga acaccacctg
    12181 ccagagctga atatcacggg cgcggaaagc acctgcacaa gcattcagat aataggtaaa
    12241 cattcgttta aagcgttcac tgtagttatc tgcaatttct ggccatgcgg cgaggaatcg
    12301 ttcataccac gccatcaacg tagtatcgta atcagcaccg aagttatgcc agtcttccat
    12361 cacaaagtgg ggttcgctgg actgagcaat ctggcgtaca gagggcaggc aaccgttcgg
    12421 aaaaatatat ttattaatcc agggatcaac attcagatcg gtttttttcg aaccgatagt
    12481 atggagcagg aatatacctt ccggtttcaa attacgatcc accaccgcaa aataggtatc
    12541 gtaatttttc ggtccgacgt gctcgaacat ccccacagaa acaatacgat caaactggtc
    12601 gttcaggtca cgataatctt gcagcaaaat ggtgacatcc aggccttcac agcgttcctg
    12661 agccattttt tgctgttcgg cagaaatggt gacgccgacc acgcttacgt catagttaga
    12721 tgccatgtag tgcgccagtc cgccccagcc gcagccaata tccagtacgc gcatccctgg
    12781 ttttaactgc aatttttcac aaatcatttt gagcttcgcc tgctgggcag attccagatt
    12841 atcggcatct ttccagtaag cgcaggaata ttgcatgaag ggatcaagca tgcgactgaa
    12901 caagtcatta cccaaatcgt aatgctcttt gccgactatc caggcacgtt ttttactctg
    12961 cagattgaag agacgggcgc ctgcaatacg cagcgtgtct ttgaaatgat gggggagttg
    13021 gttctcgaga cctgcgcgta agactttgct aaaaaacata tccagtcggt cacattccca
    13081 ccagccatcc atataacttt cgcctaaccc caaagagcct tcttgcagaa cgcgtttaaa
    13141 aaaatcgggg tttttcacac gaatatccgc cggggcagaa ccgttaatgg ctataccggc
    13201 acggctaagt aattcgttgg cgatacggta ccagttgtca tccggtacac tgacttcttc
    13261 tatacacgat gaactcatag tttctccatc actggtcggt gatcagaacc ttaaaacagc
    13321 gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt ctataatccg
    13381 acgtagaaca gagggaaagg aggaaccctt gccgaaatga ccatccatag tgagtcggga
    13441 acgatcctgt ccccgtaaaa acataatatt tatcgtagct ttaacaactc gaaattagta
    13501 taggccgcta atttcaatca tacaacaaaa ttttgttagc agcctaagta taagtatatc
    13561 gatatagatc agtgtgattc gctatgagcg acttcggcat tgccatgatt ctggcagcca
    13621 acttcttcac aacgttgcat catgtaaccc agcgcgacca gcactactgt tgataacatc
    13681 acgctggtgg tggtgagcaa tggcgtgctg atactgatca gccaggaaac taccagactt
    13741 gcgaggaagc acagacccag ttgcagagtg ttctgcaacg ctgcggcgcg accggttgca
    13801 tgtgggaagg gacgcagcgc ctgggcgaca acaatagggt agatcgcgcc attggcaatc
    13861 gccatcacac agaatgggat caggatttcg accagcgaca catggctaat gaagcccgca
    13921 gcccaggtcg caatgacgct gacagcaaac agcaccagca accacggtaa taactgcttg
    13981 ccttgccatt tctgcagtgc ggcgcgacag ccataaccac caatcagaaa cgcgatagtt
    14041 tgcgggacat aacttaaacc aataactgcc gggctgtagc ccatttcact aaggatgaac
    14101 ggtgaaccgg tcagccatgc aaaaaaactg gctgaacagg ctgcatatat cagcacgttg
    14161 ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg actattgtta
    14221 cgggccttcg tcgtgggttt gagccagaaa ataggcagaa tcagcaccac ggtaatggca
    14281 aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca gcagccagct tcctaacagg
    14341 ggagccagtg ccggagacag acccaccagc ggcatgatgg tcgcaaaaat acggttgact
    14401 ttctgtgaag gataataatc tgtcactaat gcttgccaga taaccgccgc ggcgcagacc
    14461 cccacagcct gtacaaaacg caataccagc agcgtagcgg cgttttctac ccacagcatc
    14521 cccagactac ctaacgcaaa aattgtcagg ccgattaata acaccggttt acgaccataa
    14581 cggtcggaga gtggccccca cagaagctgg gctgcggcaa aaccggcaag gaacagacta
    14641 aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc ggcgaaagca
    14701 ggcagataca tatcggttgc cagaaaaccg agtacgctca aacccgccag ccagactaaa
    14761 aatcttttcc caggttgcat gtatattctc taaatctctc aattaaacac cgccgcaaag
    14821 tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg atgactggtt tcaaaaattt
    14881 tgcaggcaga ctatgtggtc agaatattca ctcgaagttg ttgatgcggt agcgcgtaat
    14941 ggtagtttta gcgctgcggc acaggagctg catcgcgttc cttctgcggt cagctatacc
    15001 gtgcgtcagc tggaagagtg gctggcggta ccgctctttg aacgacggca ccgtgatgtg
    15061 gagctgaccg ctgctggagc gtggtttctc aaagaagggc gctctgttgt caaaaaaatg
    15121 cagatcaccc gccagcaatg tcagcagata gcgaacggct ggcgcggtca gttagctatc
    15181 gcagtggata atattgtcag gccagaacgt acacggcaga tgatcgttga tttttatcgc
    15241 cattttgacg acgtcgaact tctggtcttt caggaagtgt ttaacggtgt ctgggatgcg
    15301 ctttccgatg ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc ggtaggcggt
    15361 cgttatgcct tccgggatat ggggatgcta agctggagtt gcgttgttgc cagccaccac
    15421 ccgctggcgt tgatggatgg cccgttcagc gatgatacgt tgcgcaactg gccgtcgttg
    15481 gtgcgcgaag acacctcgcg aacgctacct aaacgtatta cctggttgct ggataaccaa
    15541 aaaagagttg tcgtgcctga ctgggaatca tcggcaacct gtatctcggc aggattatgc
    15601 atagggatgg tgccaacaca tttcgccaaa ccgtggctta atgaggggaa gtgggtggca
    15661 ttagaactgg aaaacccctt tccggattcg gcatgttgtc tgacatggca gcaaaatgat
    15721 atgtcgcctg cgttaacctg gctgcttgag tatctcggcg atagtgagac gctgaataag
    15781 gagtggctgc gggagccgga agagactccc gcaacgggtg attaacgacg atagtcgcgg
    15841 aacgggccgt cagccacgga gcggcgttca atcaagcgcg gatgcacttc aatggactgc
    15901 ggttcttcac gtttgttgac gatacgatcc aacagcatgt tgaacgctgt ttcacccagc
    15961 gaatcttttg gctggtggat cgtggtcagc gccggcgtaa aatagcgggc gttgcgcacg
    16021 ttatcataac cgatcagcga aacatcctgc gggacgcgca gacccatttc atcagcagca
    16081 caaagtgcgc ccattgccat gatatcgcca ccacagaaga cggcagtagg gcgatgcggc
    16141 tgcgacagta tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc accctgaaca
    16201 atccagcttt ccggcacctt gatcatcgct tcttccatcg ccttcataaa accggcaagg
    16261 cggcctgcgc cggtgttacg ttccagcggg ccggggatga cgccgatttc gcggtgaccg
    16321 cgttcaatca gataacgccc ggccatgtag ccgccttcga acgcgttatc aatgaccgca
    16381 tcggtgaagt cagcttttgc ttcaccccag tccatcacca ccattgggat atggcgatac
    16441 tcttccagca tcgccagcaa cggctctggg tactcagaac acatcaccag cagaccatcg
    16501 acgcgttttt gcgccatcat cgacagataa gcccgctgtt tctcaagatt gttccacgca
    16561 ttacccagaa tcagggtgta acctttctgg aagcaatttt tttcaaccgc ttcaatgatc
    16621 tcggcaaaat aggccgcttc gctgctggtc gccagcaaac cgatagactt ggtgtggtta
    16681 accttcaggc tacgcgccac cgcgctaggg gagtagtgta attctttaat cgctgcccac
    16741 acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg tgacacagtt
    16801 gtagtggaaa cgtttgctcg tttcgctaca tcttttattg ttgccattcc atttcactcc
    16861 agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta cacaccttac
    16921 gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg aaggggtcaa
    16981 tggccagtac gcaaatagtg taagcgtgga attttggccg atcttgacga aaaggggaag
    17041 tggtaaaaac acttatcatt ataaatgacg ggagattttt tcatcacagt gtgtaaaaat
    17101 gcgtaatatc actatttgtg tggtaataaa aaaggagaaa acttgatgag caccgacctt
    17161 aaattttcac tggtaacaac gattatcgtc ctcggtttga tcgtggccgt gggtttgact
    17221 gccgcgctgc actgatttcc tctcgaggga gcgcgttctc cctcgcatct ccttcctgtt
    17281 tgttagcaaa atggcaaaag tgttttctct ttttgttatt ttcatttttt tgtgattgct
    17341 gtcatgcttt cgccacttta atgaatatcg cgtaatgacg cgaagaggtg gagataaggc
    17401 atgaaaatta actatccgtt gctggcgctg gcgattggcg cgtttggtat cgggacaacg
    17461 gagttctctc caatgggttt gttgcccgtc attgcgcgcg gtgtggatgt ctcgattccc
    17521 gctgccggaa tgttaatcag tgcctatgca gttggcgtaa tggttggcgc gccgctgatg
    17581 acgcttctac tttctcatcg tgcccgccgc agtgcgttga ttttcctgat ggcaattttc
    17641 acgctcggca acgtactttc cgccatcgcg ccggattata tgaccctgat gctttcacgc
    17701 attttgacca gcctgaatca cggagcattt tttggtttgg gttcagtcgt ggccgcaagc
    17761 gtggtgccaa aacataaaca ggccagcgca gttgccacta tgtttatggg gttaaccctg
    17821 gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg aaaccatcgg ctggcggatg
    17881 tcatttctgg caacggcggg gctgggagtg atttcaatgg taagtctgtt cttctcatta
    17941 cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt attaatgcgt
    18001 ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag ctggtgcaat gtttactctc
    18061 tacacctata tctctccggt actacaaagt attacccacg caacaccagt gttcgtcacg
    18121 gcaatgctgg tgctgattgg tgtcggattc tctatcggta actatctcgg cggcaaactg
    18181 gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt tgttgttgat ggtgattatg
    18241 ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag ctattagcat ggtggtgtgg
    18301 ggcgcagcaa cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg tgtcgccagt
    18361 gaagcgccag gtctgtcttc atcagtcaat attggtgcct ttaatcttgg aaatgcgctg
    18421 ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg gatacagctt tgtgccggtg
    18481 atgggagcga ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc cagaaaacaa
    18541 cctgaaacag tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg ataaccttct
    18601 gcgttacagt tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta acgcctcatc
    18661 cgcgttggca tggtaagcga agcgcatcag gcaatgctgc atttgccatc agttattatg
    18721 cagcgagatt tttcgctacg aattcccagt tcaccagcgc ccagaagtgc tccagatagc
    18781 caggacgtgc attgcgatag tcgatgtaat aagcgtgttc ccagacatca acggtcagca
    18841 gcggagtcgc atcggtggtc agcggagtac ccgcgttaga ggttgaaacg atagccagtt
    18901 tgccatcgct gtttttcacc agccaggtcc agccagaacc aaagtttttg atcgctgcat
    18961 cagtaaactg cgctttgaaa tcggcaaagc taccaaaaga tgcggcgata gcttcagcga
    19021 cttttccagt cggttcgcca ccggcgttcg gtgccaggca gttccagtag aaagtatggt
    19081 tccagacctg agctgcgttg ttgaatacgc caccttcaga gctgcgaata atctcttcca
    19141 gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg acataagtct
    19201 gatgatgctt gccgtagtga tactcgatag tttccgcaga aatgtgcggt gccagagcat
    19261 ctttagcata tggtagtgca ggtaattcga atgacattgc tactctcctt tattattaat
    19321 ttgcatacgt acaatagcct tattgtgcgt atggataggg tagcaaatta aaaggtgaga
    19381 caaaagatga acttaccctg ttgccgtaac aacagggtaa agttttagcg aagtgttttt
    19441 ggggtcatta cccgacgagc gccaacatag tggcgctgcc agtagtcttc actgagagaa
    19501 gtgatttgaa tttcctgacc tgtgcgcggt gactgaataa atttgccgtt gccgacatac
    19561 acgccgacat gatcggctgt gccgcgtccc tgagtacgga aaaagaccag gtcgccgttt
    19621 ttcagttcac tacgttcgat tggcgctgca tcacgcaggt gatacatttc attcgccgta
    19681 cgcggaatac gaattttcac caaatcttta taagcgtaat aaaccaggcc gctgcaatca
    19741 aaaccggtac gcggtgagct gccaccccaa cgatatggct taccaatttg ctgcatcagt
    19801 ttattcattg ccactttcgt agctttttgc accttcgctt tgtgcgcgtc ggcaagagtt
    19861 cctgacgcgg catttttgac tttcacacaa tgcgatttat agcccttacg tttggtacac
    19921 ttttcggtaa agctggctga ggccgtttta ttgacggcgt gtttgctgcg agttttggcg
    19981 tttttagaag cggtaatgga agatttttta acggaagaag cggttttgct tttggtcgta
    20041 cttttactgg cggctttttt cgctgttttc tggctggctt tttgggtctt tttggtggtt
    20101 gccgtgcttt tctttttatc tgcttttttg gtgatatgag tggtagcaga actctccctg
    20161 gcttgctttg aagcatgggc cataggcgtt aaaggcaggg tggtaaaaag taaagcacag
    20221 agcgtgatcg aaatacggtt tatccgcgcc actgagcgtc cctctgttaa ggcaggtcta
    20281 tcatgcctgc gtctgattta caaaacttga tgattctaat gcatagaaga agagaaagtt
    20341 aatagctttt attgttaaaa aatacgtttc gttggcgagc gcaaaaaaat gcagcagtga
    20401 aagctaaaaa tggttataaa tacgccagtt tgccgagcat ctgggaagaa acgaatattt
    20461 aaaatgcaac aaatagagca aagggataaa atagcaaaag cgtgatgaaa atgttatttt
    20521 ccgatgccgc actgacccgc tacaatgtca gattactgcc gtaaagaagt ttgaggaagc
    20581 aagacaatga gcaccactat cgaaaaaatc caacgccaga ttgctgaaaa cccgatcctg
    20641 ctgtacatga aaggttcacc gaaactgccg agctgcggtt tctctgccca ggcagtccag
    20701 gcgcttgccg catgtggcga acgttttgcc tatgttgata ttctgcagaa cccggacatt
    20761 cgtgcggaac tgcctaaata tgctaactgg ccgaccttcc cgcaactgtg ggttgacggc
    20821 gagctggtag gcggttgtga tatcgtgatc gaaatgtatc agcgtggcga actgcagcag
    20881 ctgatcaaag aaactgccgc taaatacaag tctgaagagc cggacgcgga ataatccgtc
    20941 ctgcaatact gacggatgcg aacgggcatc cgtcagtcct ttctctatcc ccaatccagc
    21001 ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg taaacatcgg cgtttgccgg
    21061 accggtagat cattcacttc tgttagcgta aagcgcagcc gtggttcgcg acgcagtgcg
    21121 gtagtcagcg cgtggaaaac ctctggggcg agtaattcct ctttttcctg ccagaccagc
    21181 atttttttgc caccttgcgc cagatagagt aacaatttgc cgccagaaac gaccaccagc
    21241 gcacccgccc gacgcgttgg aaccagcgtt gccggatgtg caggccaggg cagaagattt
    21301 ccccacacat tagccggatc gttggcagag agcgccactg gtgtatagtg gcgcgtttgc
    21361 gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca gacgttcagc gaattgcgcg
    21421 ccacccagac cttctacaaa acgacctcgc ataattcgcc cggagtcttc catacttcga
    21481 caaagcgttt gcatcgatgg aaacccgtca gggatatttt cggctatcac cgcctgacga
    21541 ctgatgatgc cgtagcggtc gagcatattt tccgccagcg ccagcatcct ttcggtatcg
    21601 tttagtggct ccacctgcaa taacgaccag cgtccagcca gatttggtgt gttgtaagat
    21661 accagcggcg agacgggttg cgcatagaca ggacgtccac gacgagcccg gtgactgcgg
    21721 cgagttgagg tgcgtgcgtt ggaactgctg cgggtgaggg cgcgtaacgg tgcccaaatg
    21781 tcgctggtga tgacgccttg ccagaccagc gcccataacg cctcttgcag ggcagagaga
    21841 tcaaccgatt cgccgatttt gtcgcgtatc cgttggctga tttgttgtgc aaaccaggct
    21901 cctccgtcag ccagaacagc gattatcgct tgctgcagcg ccgaacgatt cgcctgatcc
    21961 gcttcggcgg gagtgaacga ttctgcggca tattcctgta gatgcagtgc caccaggccg
    22021 tcatcttcac ccagcttttt ttgtcccgac cagataaccg ctccggttgc cagtaattca
    22081 tcgagcattt ctggcgaata gtcgcgtacg cgggcaggca ggatctggct ttcccagagt
    22141 gaggcgggta aaccgactcc ggcaagctgt tcgatcaccc gcatcacgcc atctacgccc
    22201 tcataaacgc ctggcgatgt tgaggcaaag agcgccgggc taccatcggt ggcgggtaat
    22261 acgccctgac gttccagcag caatcgcgca taggtcgtgg ctgcaacggg acgcgtcgct
    22321 tctctggcgg cttgcagcga gcgcaaacgc agacgacgaa atacttcatc gctcacccag
    22381 atgtcttgtt gcagattcat caccagaccc tgttcacgca gttgctgaag ctgctcttcg
    22441 acaatggcaa taccgagact aaactcatga gccagttgtt cagccgtgac cagagcatga
    22501 gcccggagat agcgcagaaa gaggtcgcga agcgggtaac tgactctatg taaataaatc
    22561 tctggcaatg actctggtag tcgtacgccg agggcatcac gcagcctggc ggcatcatcc
    22621 atacaggcaa gacgctcctg tccgctaatc atcgctggga agattcgttt tactgcaaga
    22681 agattttcca gatacgacgc aacctcttca ctgctgcctg tatgccgttg cgccaggtct
    22741 tcaacggtca ttggccccag ttcgcgcagc aggtcgaaca atccttcttc acctttcgct
    22801 cttctgccag gagccagtcg ttgcaactct tcttccacct ggcgaatgac ctgcgggtcg
    22861 agtaattccc ccggatcgac ctgtccgagt agattgcgca gtaactcgct gtccagcgac
    22921 agtacggatg cccggcgctc tgccagcggg gcgtcgctct ggtacataaa ttccgcgaca
    22981 tagccgaaca ataaacttgt ggcgaaaggc gagggcgtag tggtcgttac atcggatatt
    23041 tgaatttcgc caccgttcag gcgacgcatc aaacgttcca gtgcgggaag atcataaaca
    23101 tcttgcagac attcgcgtag ggtttcgaga atgaccggaa aatccggata tccctgagcg
    23161 atttccagca actgactggc gcgcagccgt tgttgccaaa gcggggtgcg atggcccgga
    23221 gtgcgccccg gcattaataa tgcccgcgcg gtgcattcgc gaaaacgggc ggcgaaaagt
    23281 gccgagctgc ctaccgcctc gcggacaatt tgcagcaact tttctggttc aaacaaaaaa
    23341 atcgcggcgt cgggcaattt accatcagtg tcaggaatgc gcgcaacaat gccgtcatca
    23401 ctggcgacca cagacgcgtc agcgccccat agggcatgta ttcgcccggc aatcgccagc
    23461 gcccagggtt catgcacccg tcttccatag ggagagtgca aaataatacg ccagtcgcca
    23521 atttcatcac ggcaccgttc gaggaccaga tggcgactcc ccggaacgat gcccgtcgcg
    23581 ttgcgttgct cgtcaatcaa cccctgaata ttggcgttcg tattttcttc tgccagccac
    23641 gggggaatag tcccggaaaa gaacgcgcca tccgccagta aatgaagaaa atcgccgatc
    23701 atctcgccta attcagccgg acgtccgtta ccttcaccac gccagaaggg gagccgggca
    23761 gaacgacccg gagcaggagt cacaatcacc tgatcgcggg tgatttgctg gatccgccat
    23821 gaggtagcgc cgagagtgat aatgtcgctc acccgcgact catataccat ctcctcatcc
    23881 agttcaccca cccgccgcga accggcctgt tcttcacctt cgggtaataa cacgctatac
    23941 atgccacgat ccggaatggt gccgccgctg gtaaccgcca acaattgagc gccaggtcgg
    24001 gcggtcaata tcccggtctc cctgttccag accagtttgg ggcgaaaagc agaaaaatcg
    24061 ccagagggat agcgcccgga aagcatatcc agcgtggcgt caaaaacacg tcttggcaga
    24121 tctttccacg gtgcggcacg gcgtacgcgg gagtaccatt cgtctacctg taatgcatcc
    24181 atcgccgcgg cggcaacggt ttgctgcgca aggacgtcga gtggattatg cggcggtgtc
    24241 aggttttcca gcctgcctgc gaacatacac tctacaatga ctgcggaatc gactaagtca
    24301 cgccgggtac gagggaaaaa cagcccttta gatacaccgc caacctgatg tccggcgcga
    24361 ccaatgcgtt gtaacccact ggcaacagaa agcggcgttg ccacctgaat caccagatcc
    24421 accgcgccca tatcaatccc cagttcgaga ctggaggttg cgaccacgca gcgtaattca
    24481 cccgatttca gcgcctgttc ggtgattgct cgttgttctt tagagacgga gccgtggtgt
    24541 gagcgggcaa taaaaacgtc gctactttgt acatggttag aggttgcgcc ggaggtcgac
    24601 tcgaaatggg ccgtatcaac ggcgatagac ggggaacgct gtaagcgtgc ggcgtaaagc
    24661 tcatttaatc gtgccgtcag tttttccgcc agcccacgtg aattagtaaa gacaatggtc
    24721 gagcgatggc gcaacacttc atcaaggata cccgtttcaa tatatggcca gatggagcct
    24781 tcccggccgg catggctgtc ttcgccggtg ccgctggcga ccgatgagac atcatccata
    24841 ttggcgaccg gtacgacaat tcgtatctgc ggatggcgca ttgcgggcgg gttgactacc
    24901 gtaaccgggc gatcgccacc aagaaatgct gccacatcgc tggctgagcg cacagtggca
    24961 gaaaggccaa ttcgctgtgc tgaggtgtgg agcagcgcat cgagccgctc cagacttaac
    25021 gccagatgcg caccacgttt actgcccgct accgcgtgga cttcatcaat aattaccgtt
    25081 tcgacgccgc gtagcgtttc gcgcgcgcgg gaggtcagca tcagatagag tgattcgggt
    25141 gtggtgatca gaatatccgg cggattacgg gtgagtttgc tgcattcctg tgcaggcgta
    25201 tcgccagtac ggatccctac gcgaagattg acttccgttt cgccgcgccg ccgccgttca
    25261 tcggcaatac ccttcaacgg gagctgcaag ttgcgctgaa cgtcggtgcc cagagctttt
    25321 atcggtgaaa tatagaggat gcgtgaggtt ttacgcttat gcgcctcgcg ggtatcttcg
    25381 ccgccttcgc ggaagagccg atcgagggcg tagagaaatg ctgccagcgt tttcccggag
    25441 ccggtcggtg caatcaccag cgcatgttcg cttcgcgccg ccacatgcca ggtttgcgac
    25501 tggacagcgg tcggctgttt aaaggcgcga agaaaccagt cgcgggtcgc cggtgaaaac
    25561 acgtccggca ggagcgatga agggtctgga ttatctgcca ttaacgctat tccccgtgaa
    25621 tatcatgctt gatgcgtgga atagcctcac tatagatgcc tgaatcattg catgtcatca
    25681 ggcatcgact cgattacacc tcttcggcgg caggtagcgg ccagcctccc agacgtttcc
    25741 agcggttgac gatttcacaa aacagcacag cagtgcgttc ggtgtcgtac agcgcggagt
    25801 gcgcctgggt gctgtcgaag tccatgccag cggtctggca agcctttgac aatacggttt
    25861 gtccgagtgc cagcccggcc agtgcagcag tgtcaaaagt ggcgaaaggg tggaacgggt
    25921 tacgtttcag tgaggcgcgt tctgcggcgg ccatcataaa gctgtgatca aaattggcat
    25981 tgtgcgccac cataatggcg cggttacagc cgctcgcttt aataccttta cgtacaactt
    26041 taaaaatttc gtgcagcgcc tcgtattcgc tgaccgcgcc gcgatcggga tcgttcgggt
    26101 caatgccgtt gaaggcgagg gcctctggct gcaaatttgc gccgacaaat ggttcgacgt
    26161 ggaaatgtaa tgtggtgtcc ggcatcagcc agccttgttc atccattttc agggtgatgg
    26221 cggcaatctc aagcagcgca tcggttttgg cgttaaatcc ggctgtttca acatcgatca
    26281 caacaggata aaaaccacga aaacggtcgc acagaccggt aagttgagcg ttatcggaca
    26341 tctgattctc ttaatacgga taaattgcag cgcgcattat gacaaatatt acttgtcgat
    26401 gcagtaaaga tgcgggcgcg atgagttcac gcccggcagg agattagttg cccagaccgc
    26461 gaccggcgtc tttctcttcg attaactcaa ttttgtaacc gtccggatct tccacaaacg
    26521 cgataaccgt agtaccgcct tttaccggac ccgcttcacg ggtcacgtta cccccgtttt
    26581 gacggatttt ttcgcacgct tcagcggcgt tatctacgct aagcgcgatg tgaccataag
    26641 cagtgccgag ttcgtattta tccacgcccc agttgtaggt cagttcaatc accgcttctt
    26701 cggtttccgg gccgtagcca acaaacgcca gtgagtattt gtattccggg ttttcgctgg
    26761 tacgcagcag tttcatgccc agcactttgg tataaaaatc gatggagcgt tgcagatcgc
    26821 caacgcgcag catggtatga agaagacgca tctttttatc ctcaaaatgg ctaacagatt
    26881 caaaatgttg ttttagtata gcggcggctt tacgccgctc gcaatgttgg attacaacgt
    26941 cgggtaatcg gtatagcctt ccgcgccgcc accgtagaaa ctttcggcac gctgtgggtt
    27001 aagctcagct ttgcgctgca agcgggcgac cagatccggg ttcgcaatcc agtcacgacc
    27061 aaatgccacc gcatcaatta accctttgcc gatcagcgtt tcagcttttt ctactgtgta
    27121 tgcacctgcg ccgataatcg gaccgtggaa acgggcgcgt actttttcgc ggaacgcatc
    27181 agtatacggt tcaccccccg cccaatctgg ttctgacata tgcagataag caatgccgcg
    27241 tttacccagt tgttcaatca gatacagtgc atcggcttct tcattcgcgc cgttatccgt
    27301 gttctggaaa gtaccgattg gcgaaacgcg aatgccaatg cgatcggcac cccattcttc
    27361 aatcccggca tcgaccactt ccagtaccag acgtgcgcga ttttccacgc tgccgccgta
    27421 ctgatcggta cgatggtttg aagaaggaga aaggaactga tgcagcaaat aaccgtgagc
    27481 agagtggagc tctaccagat caaaaccggc ttcacgcgcg ttagcaatgg cctgacggaa
    27541 atcattgacg atacctggaa tctcttccag ttcaagcgca cgcggcatgg atgtttcaac
    27601 acggatcgcc tgaccatttt catcgcgcag agaagtacgt gttcccgcgc taagtgctga
    27661 aggcgctacc ggtgcctgac cgccaggttg caggctggcg tgagaaatgc gtccggtgtg
    27721 ccacagctgc acggccatat gaccattttc agcatgaacg ccagcggtga tttttttcca
    27781 tgcggcaatt tgctccggac tatggatgcc aggcgcacct gcatatcctt ttgcctgggc
    27841 agaaatttgc gtggcttcac taataatcaa accggcactg gcacgttggc gatagtattc
    27901 cgccatcaac ggggtaggaa tgtcacccgg ttcaatactg cgcagacgcg tcagcggtgc
    27961 cataaaaata cggtttgccg ccgtgatcgc gcccactttc agtggggaat acagtttttc
    28021 agatgacata aaggctcccg gtcgtctggt gataaaaaat gcctgctaaa cggcaggcgt
    28081 cgcaataatg ttttttacat gggccagcgc gttttccagt ggctcgaaac tgcgcgaaat
    28141 tttggcctgc agattcgcac ccagccatag tgcgtaaagc acttgtgcct gttgtagcgg
    28201 ttcgccacaa aaggttaaac aatggttctc acggccattt tccagcgcct gagagagcag
    28261 ggcgatcacg ccgcgagcac ctttatccat cgcgctgcgc atatcttctg acagatcgca
    28321 cacttcggca gagagtttta ctgtcaggca accactgatg gttccatgtt ggcaaaactg
    28381 gttcagtgtt tgctggtaat aagccagtat gcggtcgcgg tagttacctt cgccggattg
    28441 cagcaactca gtcagtcgct ggtgatatgc ggcgtaatga cgctcaagca tggcaacgcc
    28501 aaacgcttct ttagagcgaa agtagtgata gaaggaccct ttcggcactt cagcggtttt
    28561 tagtaattcg cttagcccca tcccggtgaa tccacgttgc aggcaaagtt gctcgcccgt
    28621 cgccaggaga tgttcgcgag tatcatgttc ggtgtgtttg ttcatgcgtt ggagtgtagt
    28681 agaccagtcg gtctagtgca agcaaagttg ctactttgga cattaacagc ttcaataatg
    28741 gtaataatct tatctgagga gggcgaagtg gcggagcaat tagagttctt tcccgtccag
    28801 agcccgtgcc ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcgt
    28861 agtcgtgatg agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg
    28921 cgcctttgtc ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc gtcatcagac
    28981 gaaccggagc aaccatcact cttttgaacc taatcttgcg tatactcctc tcatatctga
    29041 taagaggaag tgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc
    29101 cgttttgtga tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc
    29161 agttttattg aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat
    29221 ggtggctatc agtgcgaagc ggcgtttggc gaggcactga aactggcacc tcacctgcgt
    29281 gaacggatgg aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc
    29341 attggtcatt acatcactga ccgcgatcac atcattaaga gcgccgaaca gtcgctaatt
    29401 aatctcgcga ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat
    29461 gccgatgaag tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt
    29521 ggcgtatcga actttacgcc tgcgcaattt gccctgttgc aatcacgtct gccgtttacc
    29581 cttgccacta atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggcacg
    29641 ctcgaccaac tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt
    29701 cgtctgttta atgatgatta tttccagcag ctgcgtgatg aactggctgt ggtggcagag
    29761 gagttaaacg cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccatcg
    29821 cagccgctgc caattatcgg ttcaggtaaa attgagcgcg tacgggcagc tgtcgaagca
    29881 gaaacactga aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc actggggtac
    29941 gacgtaccgt aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac
    30001 ttattcagga atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gctattctgg
    30061 cgctggttgt tgcaaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca
    30121 cgtcgcaagg ggtagggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc
    30181 tggagttttc gcccgatctg aaagcattac cccccggtga acatggcttc catattcatg
    30241 ccaaaggaag ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag
    30301 gcgggcatct tgatccacaa aataccggta aacatgaagg gccagaaggt gccgggcatt
    30361 taggcgatct gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg
    30421 cgcctcgtct gaaatcactg gatgaaatca aagacaaagc gctgatggtc cacgttggcg
    30481 gcgataatat gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg
    30541 gtgtaattaa gtaattaaga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa
    30601 agcgagcagt acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga
    30661 cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc
    30721 tgaacgccac gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgc
    30781 gataacggat cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc
    30841 agcagcacta caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc
    30901 aactgactga cgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg
    30961 cggcttagct gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg gcttttacga
    31021 gcatccgatc ctggacgcag aatggcgaac gctagccaag ccagcgcaac gccaacgatt
    31081 tttgccagat tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg
    31141 ataaacgaac ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc
    31201 aatttaagaa gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga
    31261 tcgctaatct ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc
    31321 agcgtgcgca tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg
    31381 cagctaattg ctgccagcgt taatgcattg gcaccggcat cccattgcga tgcaatactc
    31441 catgcgccaa tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat agcttcggcg
    31501 ttgtcggtat gacgagccag agcggatgtc cgtgcgagtc tggcatgatc atctacaccg
    31561 ctttgcagac gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt
    31621 cgctgccaga aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata
    31681 atacgtgcga cggtgtaagc atctgtttgc gaactggcga gcgccgtcag caattgttcg
    31741 agaatttctc gcgtggcacc tggcggtgag ggccagttga gcaacatacg tcgcaggctg
    31801 gagatgacac tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc
    31861 tggcgaaaac gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc
    31921 tgcccaatca ccccctcatg tgctgcacga atggcatcgt ttgtttcagg ctgccagagt
    31981 aaactggcat gttccagtaa tcgggcgtgc atgtttttca atgcggttaa aagggctgta
    32041 gcatcggaac tgctaggcag gatcatcatc atcatgccgc cgcacaaaat accgactatc
    32101 acctcgcaaa cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc
    32161 atcggaaagg cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta
    32221 ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc acgccgacat gctcaataga
    32281 aaaaaccacg gctcattgag cgtatgcccg gcaagaagca gtgccgcaat ggctcccagt
    32341 aaactgccag cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta
    32401 accactgcag ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc
    32461 gtcagcgcca gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg
    32521 gccttgaacc agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg
    32581 caatgctaca ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa
    32641 agcggacggg aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa
    32701 taaggcttga gtcactttca acgctttgat cataaatcgc ccgaccgata ctggaaacgt
    32761 gaccccgtaa cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga
    32821 tatggcgcaa cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa
    32881 gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca
    32941 cccaaccact caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca
    33001 gcgtggcctg tgcggcatta acactggcct gcatcgcttt aacattgagg ttagcggtat
    33061 cgagttcttc ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat
    33121 tggcttttgc gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga
    33181 tctgaaaggg cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt
    33241 cttttatatt cagctcaaca atacgtcccg acacctgtgg ggtgatagaa accttttctg
    33301 cgcgtatttt cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc
    33361 cggcaagaac cgcaactatc gctacaatga ttgttgagaa atacttaatt gttttaattg
    33421 acatagttac cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga
    33481 taaatccatt aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg
    33541 gtgtacgaca agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaaata
    33601 gacggacgca ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt
    33661 cacaaagtag attcctttac gaccgaatat attgcgttag attaataaat attctttaag
    33721 tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat
    33781 acatttgttt tagcaataca attgctgcac actattctaa aagccgcata atatcttagc
    33841 aagctaatta taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg
    33901 tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc
    33961 attgggttac gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg
    34021 caaaagcgat tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa
    34081 aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga
    34141 cggaaaaggc agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg
    34201 aaatattaca tggcatctcc gcagaggaac tggagcaact gattacgctc atcgcaaaac
    34261 ttgagcataa tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag
    34321 taacctgacc acacgcaaac ttatttagcg cggagaaacg gtcacctgac tgccattgct
    34381 ggccagtacg acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat
    34441 gatggtatta ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc
    34501 actctgtacg ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga
    34561 acgcccggtt ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat
    34621 tgcaccgata acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat
    34681 ggtgccatag ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga
    34741 cagggtgtcg ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac
    34801 gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg
    34861 gtcaaagtat atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc
    34921 ataatgaaag taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa
    34981 gataatgttt tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt
    35041 tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc
    35101 tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaata
    35161 gatgaacacc gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa
    35221 caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt
    35281 gatactcaac tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac
    35341 ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg
    35401 acgggcgtgg aaccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc
    35461 ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca
    35521 ccgctggtac ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg
    35581 ctcaatattg gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc
    35641 tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa
    35701 ccttacgata aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa
    35761 aatatgctca gcgacccata tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac
    35821 tttaactacg gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat
    35881 gttcaggcca cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc
    35941 ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg
    36001 cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt
    36061 ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta
    36121 ccaggaaatc tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc
    36181 cctgctaacc cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact
    36241 tggaacgtta gggagggcgt attgccctcc agaccaggaa agtcttcggg aaatgtctat
    36301 gaaaaaactg ttactgatct gtttgccggt gcttctcacc ggctgtagcg cgttcaatca
    36361 actggttgag cacatgcaaa ccgctacgct ggaataccag tgtgatgaaa aaccgttgac
    36421 ggtcaaactg aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca
    36481 tctcaaacag ggcatttcag cgtctggtgc acgttacact gacggaatct atgttttctg
    36541 gtcgaaaggc gatgaagcga ctgtctataa acgcgaccgc atcgtattga ataactgtca
    36601 gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact
    36661 gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc
    36721 gccgtgaata caccaaaggc gggttacgcc gtcgcgatct tcccgccgat ccattaaccc
    36781 tttttgaacg ttggctctct caggcgtgtg aagccaaact ggcagaccct accgcgatgg
    36841 tggtcgctac cgtggatgaa catgctcagc cttatcaacg catcgtttta ctcaaacatt
    36901 acgacgaaaa aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg
    36961 aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg
    37021 tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc
    37081 cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg
    37141 gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat
    37201 tgcccagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg
    37261 gtgagcatcg cctgcatgac cgctttttgt accagcgaga aaatgatgcg tggaagattg
    37321 atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc
    37381 tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc
    37441 agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga
    37501 gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg
    37561 cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct
    37621 tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt
    37681 aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa
    37741 caccgaagaa actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct
    37801 cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa
    37861 tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat
    37921 caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga
    37981 gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt
    38041 ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac
    38101 ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga
    38161 tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag
    38221 cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct
    38281 gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa
    38341 cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca
    38401 cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt
    38461 gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga
    38521 gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg
    38581 tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc
    38641 cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg
    38701 tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg
    38761 agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta
    38821 tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt cctatgcgcc
    38881 gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg
    38941 gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg
    39001 ccgccattga taaattacac acctgtgatg ccgtgttaag tggctatctg ggatcggcgg
    39061 agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa agccgcgaat ccgcaggcga
    39121 aatatttttg cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg
    39181 tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg
    39241 ttgagctgga aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag
    39301 cgcgcgaact cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg
    39361 gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca
    39421 gccgtccgct ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg
    39481 gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggcgctg gaacatgtga
    39541 ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag
    39601 tggtggctgc tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct
    39661 gaaatatctc atttcggccc acaacgacct gtgggccgat ggatattact ttaagccttc
    39721 cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa atgctgcaat
    39781 gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag
    39841 ataggcatca gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgcctc
    39901 gttcacatat tgcagcttct tctccagctg cgcgcgaact gtcggtttgt actcttccgg
    39961 tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag
    40021 ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg
    40081 gacgctgtcg gcaagatact gcataatcgc tacgccttcc gtcagcaaag taccgtcatc
    40141 cagcagcaat gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc
    40201 gagacgtttt ttcattaaat ccacactgac gagggtaaaa tcctttccgc tctcacgcag
    40261 ggtgatatgg gaagcgagag agcaggcacc cggtttgtag aacaatttca tcggtaactc
    40321 cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg
    40381 aaaagttagc ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc
    40441 tttgtctgca gcgtcatcct gcgtcatacg gtgcagttta ggcgcggtca gcagcatcag
    40501 tactgcaata atggcagtag cgacaccaat ctgcaagaat acgcgaccat aaacttccag
    40561 tgacatcagc gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc
    40621 aatcaggttt gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg
    40681 ctgcggaacg agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat
    40741 gctctgcagg ccatagcttg cgaccagcca gcttacagac acgataccag cgtcagacgc
    40801 gaatttcgca cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc
    40861 aaacttggtt ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg
    40921 actaccgatg atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag
    40981 acccagaatg gagtgctcaa cgttacgaat cgcaaagaag ttcagtgatg tcggcatctg
    41041 gctgtacagc acgaagaaga tgattgcttc gagcatcagg atgaaggcaa cgatcatttt
    41101 acgacgcgca gcacctttca tcgcgaaggc ttctttaccg aagataacca cgataccgaa
    41161 ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat
    41221 agcgatcagt gccacaacac caataatggt tagcagcagg ttacggtagt tgataggctc
    41281 gaagtctggt tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat
    41341 agtgatcagc aggcctacaa cgctcaatgc aaacgcaacg ctccagccgt atttcgcggc
    41401 cagccacggc gtagcaatca tagagaagaa agagccgatg ttgacggaca tgtagtacat
    41461 ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga
    41521 cgggttagcc ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacaat
    41581 accggcgtcg tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc
    41641 gagcataatt acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac
    41701 cagaccataa accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat
    41761 acccagttgt ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg
    41821 ttcccataac tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa
    41881 actgacgctt tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc
    41941 ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata
    42001 gtgggagggg aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta
    42061 ataatctgtt acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc
    42121 gttaaatttt tcaaaaaacg taacggcaga ttttttcact gcactgagag gggggtgatg
    42181 gcttaacggc gatatgttct gctatttagc gtttttacta gtgagatagt ccagtttctg
    42241 aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt gcgtgtgtaa atttaaatga
    42301 ttttaacctg atttttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa
    42361 tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac
    42421 ggattacctg atgataacgc taagcaatgg catcattcac gttaaaaaga tgttttaaaa
    42481 gaggataaag aaaggttatc aatggggtaa tcggtgttac cccttttctt cagatgtcaa
    42541 ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg
    42601 caatgcaggt ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg
    42661 ccggaaccac ttttagtagc ttttcttcta cctgttcgac atttttcccc ggcgcaaatt
    42721 gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg
    42781 cagtgtttaa tacgacgttg gctgttttac gacctacgcc gggcagggct tcaagcgcag
    42841 cacgatcttc cggaaccaag ccattatgct gctccagtaa gatacggcag gttttgatga
    42901 tattttctgc tttgctgtta taaagcccaa tcgttttgat ataggtttta accccttcaa
    42961 cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct
    43021 tattaacact gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag
    43081 gcgaactgaa attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga
    43141 tctctaggcg ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg
    43201 ttcggcagct gcttcagtac ggcgcttttt cattctttca tcaatcaggt attttcctgc
    43261 cagcatcagt cccaggccaa taaatgcacc tggaggcagc atcgccagca ggaaagggga
    43321 gtcggtgtgg aaaatttcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc
    43381 accgtcaaac aatgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc
    43441 gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt
    43501 ggcggcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa
    43561 aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct
    43621 gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg
    43681 acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa
    43741 acccagagcg ttagtggcag tggacgtgac cgccaacaga ggacaaaggc cgagcaactg
    43801 gaccagcgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac
    43861 gctttattct ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa
    43921 tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg
    43981 gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca tcatctgcac cactgatttt
    44041 tttacccgca aaatgggtga tccagtcaga aaggcgcagt tcgattttat cgccaagccc
    44101 tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc gtgccgttaa aatcggctcc
    44161 caccagcagc tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcggc
    44221 taccggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg
    44281 cgcagttacc agatagcaac tctgtgccag cgcattgtta tagcgttcgg ctggcagcac
    44341 ctgatcaaat aatgcctttt gttgcagact ggcctgttca gcaatcgtcg ttttggtcat
    44401 ctggttgatg gccgcagtta accctgttga acccgctgca aatagcgcta acgtaatgcc
    44461 gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg
    44521 cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc
    44581 gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aagcccgcca gcgcgccgaa
    44641 aataagacga ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa taaagaatgc
    44701 gccgagcatg gtcgcaccag acagcagatg aatttgcggt gctgccagtg tttctggtga
    44761 gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg
    44821 ccagcgaatc gctttctgcc atagcaacca caggccgcca gccagccagg cgagatttac
    44881 ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc
    44941 aaccgaatga ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg cctgactaat
    45001 gccatcaata cctaagcgta gtgtgttcat atcaccacca ctggcggtat gtccgctgaa
    45061 aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa
    45121 ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg
    45181 attaaacggg ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac
    45241 cgtacccagc acgaccatcc accatggcgc gaggggggga atacttaccg ccagcaataa
    45301 gcctgtcagc aatgctgagt tatctttcaa cgttgcggct accgactgct tgcgtagttt
    45361 gagtacgaga gcttcggcta acagagcact aaccgatgcc aacaggatct gaacgagagt
    45421 accccaacca aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa
    45481 cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa
    45541 taccatttag tcctcgttta caaccttctg ctgggcggct tttttggcct gagcgcgggc
    45601 aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt tcttctggtt ctgcgttagt
    45661 ctgttgctgt tccagcttgc gcgctttggc acgggcaata gcg
//
LOCUS       DAZFDP010000019        45353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFDP010000019 DAZFDP010000000
VERSION     DAZFDP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHJ1G_001193"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHJ1G_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4763084.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHJ1G_001194"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHJ1G_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4763085.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHJ1G_001195"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHJ1G_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4763086.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHJ1G_001196"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHJ1G_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4763087.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            9267..9641
                     /gene="tomB"
                     /locus_tag="ACHJ1G_001197"
     CDS             9267..9641
                     /gene="tomB"
                     /locus_tag="ACHJ1G_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4763088.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            9667..9885
                     /gene="hha"
                     /locus_tag="ACHJ1G_001198"
     CDS             9667..9885
                     /gene="hha"
                     /locus_tag="ACHJ1G_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4763089.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            10057..10608
                     /gene="maa"
                     /locus_tag="ACHJ1G_001199"
     CDS             10057..10608
                     /gene="maa"
                     /locus_tag="ACHJ1G_001199"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4763090.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHJ1G_001200"
     CDS             10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHJ1G_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4763091.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHJ1G_001201"
     CDS             11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHJ1G_001201"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4763092.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHJ1G_001202"
     CDS             complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHJ1G_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4763093.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13423..13519)
                     /gene="ffs"
                     /locus_tag="ACHJ1G_001203"
     ncRNA           complement(13423..13519)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ1G_001203"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13681..13992
                     /locus_tag="ACHJ1G_001204"
     CDS             13681..13992
                     /locus_tag="ACHJ1G_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4763094.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHJ1G_001205"
     CDS             complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHJ1G_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4763095.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            14813..15673
                     /gene="tesB"
                     /locus_tag="ACHJ1G_001206"
     CDS             14813..15673
                     /gene="tesB"
                     /locus_tag="ACHJ1G_001206"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4763096.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHJ1G_001207"
     CDS             complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHJ1G_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4763097.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHJ1G_001208"
     CDS             complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHJ1G_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4763098.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHJ1G_001209"
     CDS             complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHJ1G_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4763099.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHJ1G_001210"
     CDS             complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHJ1G_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4763100.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHJ1G_001211"
     CDS             complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHJ1G_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4763101.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHJ1G_001212"
     CDS             complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHJ1G_001212"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4763102.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHJ1G_001213"
     CDS             22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHJ1G_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4763103.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            24427..25122
                     /gene="queC"
                     /locus_tag="ACHJ1G_001214"
     CDS             24427..25122
                     /gene="queC"
                     /locus_tag="ACHJ1G_001214"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4763104.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHJ1G_001215"
     CDS             complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHJ1G_001215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4763105.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHJ1G_001216"
     CDS             complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHJ1G_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4763106.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHJ1G_001217"
     CDS             complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHJ1G_001217"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4763107.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHJ1G_001218"
     CDS             complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHJ1G_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4763108.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHJ1G_001219"
     CDS             complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHJ1G_001219"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4763109.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHJ1G_001220"
     CDS             complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHJ1G_001220"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4763110.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHJ1G_001221"
     CDS             complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHJ1G_001221"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4763111.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHJ1G_001222"
     CDS             complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHJ1G_001222"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4763112.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHJ1G_001223"
     CDS             complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHJ1G_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4763113.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            35808..36386
                     /gene="yajG"
                     /locus_tag="ACHJ1G_001224"
     CDS             35808..36386
                     /gene="yajG"
                     /locus_tag="ACHJ1G_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4763114.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            36430..37905
                     /gene="ampG"
                     /locus_tag="ACHJ1G_001225"
     CDS             36430..37905
                     /gene="ampG"
                     /locus_tag="ACHJ1G_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4763115.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHJ1G_001226"
     CDS             38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHJ1G_001226"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4763116.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHJ1G_001227"
     CDS             39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHJ1G_001227"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4763117.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHJ1G_001228"
     CDS             41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHJ1G_001228"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4763118.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHJ1G_001229"
     CDS             41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHJ1G_001229"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4763119.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHJ1G_001230"
     CDS             42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHJ1G_001230"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4763120.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            43345..43656
                     /locus_tag="ACHJ1G_001231"
     CDS             43345..43656
                     /locus_tag="ACHJ1G_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4763121.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            43640..44182
                     /locus_tag="ACHJ1G_001232"
     CDS             43640..44182
                     /locus_tag="ACHJ1G_001232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4763122.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(44238..45173)
                     /locus_tag="ACHJ1G_001233"
     CDS             complement(44238..45173)
                     /locus_tag="ACHJ1G_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4763123.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT        10823 a        11935 c        11102 g        11493 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttgc ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga acggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaaaatact gattgttagt taatttatat
     8881 taagtagcgc taatagattt aataatccat aatcatttag aggctattct taattatttg
     8941 cggtaattct ttattcattc ctcggttatt acgtcatatt cagagcaatc ctggtattag
     9001 tgtcaccaat ttcatctggc gataatcctg aaatgttatg aacagttcga gcaaactgct
     9061 tttacctgct gcgggttagt gctaatatga aaaagtgagt cctgtcccga ttccttccta
     9121 attgtaattt ttcgtaataa tgcgatgaaa acctgcaaag agtggcttat agttaagcta
     9181 acaaacgaga gggcaagtcc aggtcagtaa gttttttcca tcccgaaagg tgtccgttag
     9241 ttcaaccact aagaagggga cgcgttatgg atgaatactc acccaaaaga catgatatcg
     9301 cacagcttaa gtttctctgt gaaaccctgt atcatgactg tcttgcaaac cttgaagaaa
     9361 gcaatcatgg ctgggttaac gacccaacct cggcgatcaa cctccagttg aatgaactga
     9421 ttgagcatat tgcgaccttc gcacttaatt acaaaattaa gtataatgaa gacaataagc
     9481 tcattgagca gatcgacgaa tatctggatg acacctttat gttgttcagc agttatggta
     9541 ttaatatgca ggatcttcag aaatggcgga agtcaggtaa tcgactattc cgttgttttg
     9601 tcaatgcgac gaaagagaat cctgcgagtt tatcttgtta gaattattac aaccatgggt
     9661 agaagtatgt ccgaaaaacc tttaacgaaa accgattatt taatgcgttt acgtcgttgc
     9721 cagacaattg acacgctgga gcgtgttatc gagaaaaata aatacgaatt atcagataat
     9781 gaactggcgg tattttactc agccgcagat caccgcctcg ccgaattgac catgaataaa
     9841 ctatacgaca agatcccttc ctcagtatgg aaatttattc gctaataaat aattcgcttt
     9901 cggagctata accggctgtt tattaagaat tttatacttt ttcaccatga agacataccc
     9961 tatgtgatct ttatcacaca gatgtaatag gaacgttctc ttcactgact tttcgtctta
    10021 ctgtgttgcc gcattttcag caaccggagg tcagtaatga gcacagaaaa agaaaagatg
    10081 attgctggtg agttgtatcg ctcggcagat gagacgttat ctcgcgatcg cctgcgcgct
    10141 cgtcagctta ttcaccgata caatcattcc ctggcggaag agcacacatt acgccagcaa
    10201 attctcgctg atctattcgg tcaggtgaca gaggcttata ttgagccaac gtttcgctgt
    10261 gactatggct ataacatttt tctcggtaat aattttttcg ccaacttcga ttgcgtgatg
    10321 cttgatgtct gccctattcg catcggtgat aactgtatgt tggcaccagg cgttcatatc
    10381 tacacggcaa cacatcccat cgaccctgta gcacgtaata gcggtgctga actggggaaa
    10441 cccgtcacca tcggtaataa cgtctggatt ggcggacgcg cggtcattaa ccctggtgtg
    10501 accattggtg ataacgtcgt ggtagcctca ggtgcagttg tcacaaaaga tgtcccggac
    10561 aacgttgtcg tgggcggtaa tccagccaga ataattaaaa aattgtaatc ggtttttcgc
    10621 aactgttatg caaaattgtg gtagatctgt tacttcccct ctactattcc cacgttaaaa
    10681 tagggtgttc cctggaaagt tgcagatacc acgaaggcaa acgatgaccg aaatacaacg
    10741 cctgctgacc gaaacgattg agtctctgaa tacccgcgaa aaacgcgaca acaaaccccg
    10801 ctttagtatc agttttatcc gtaaacatcc ggggctgttt atcggtatgt acgttgcttt
    10861 ttttgccacc ctggcggtga tgttgcagtc cgaaacgctg tcaggctctg tctggctact
    10921 ggttgtatta tttatcctgc ttaatggttt cttctttttc gatgtctacc cacgctaccg
    10981 ctatgaagat atcgacgtgc tggatttccg cgtttgctat aacggcgaat ggtacaacac
    11041 gcgctttgta cctgccgcgc tggttgaagc catcttgaac tctccgcgtg tcgcggatgt
    11101 tcataaggaa caactgcaaa aaatgatcgt ccgtaaaggt gaactgtctt tttacgatat
    11161 ttttaccctc gctcgcgccg aatcaacatc ttaagttagg gttacatacc aggcgtaaag
    11221 ctctgcgcct ggtcaaatga caatgatcgt ttccacccat cacttcatga aataccagct
    11281 ctacctcctt atctcctgcc agcctttttc cacaatcaga tatactttcc ctacactgtg
    11341 ttaataagga tatgctggtg agaacacgac atctggtcgg ccttatttcg ggagtactga
    11401 ttctttcagt attgctgcct gtcggcttaa gcatctggct ggcccatcag caggtagaaa
    11461 catcgtttat tgaagagctg gatacctatt cctcccgcgt cgctattcgg gccaataagg
    11521 tggcgacaca agggaaagat gcgctgcaag agctggaaag atggcaaggc gctgcctgta
    11581 gcgaagccca tctcatggaa atgcgtcggg tatcttacag ttatcgctat attcaggaag
    11641 tggtttatat cgataacaac gttccccagt gttcgtctct ggagcatgaa agtccgcccg
    11701 ataccttccc cgagccaggt aaaatttcga aagatggtta tcgtgtctgg ttaacatcgc
    11761 ataacgattt aggcattatc cgttacatgg tcgccatggg aacggcacat tatgtcgtca
    11821 tgatcgaccc cgcttccttt attgatgtca ttccctatag ctcatggcaa attgatgccg
    11881 ccattattgg caatgcccat aacgttgtca taaccagcag cgatgaaatt gctcagggaa
    11941 ttattaccag gctacaaaaa acacccggtg agcatatcga aaataatgga atcatttacg
    12001 atatcctgcc cttcccggag atgaatattt cgatcatcac ctgggcttca acgaaaatgt
    12061 tgcagaaagg ctggcatcgg caagtcttta tttggttacc gctcgggttg gtgattggcc
    12121 agctggcagc gatgtttgtg ctgcgtattt tgcgccgtat ccagtcaccg catcatcggc
    12181 tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta tcagccgatt gtctccttag
    12241 ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg gccgcagaca gacggtagtt
    12301 ggttgtcacc agatagtttt attccgctgg cacagcaaac gggcctttct gagccattga
    12361 cgctactgat tataagaagc gtctttgaag atatgggcga ctggctgcgt cagcatccac
    12421 agcagcatat ttcgatcaat cttgaatcca ccgtgctcac ctcggaaaaa atcccgcaat
    12481 tgctgcgtga aatgatcaat cactatcagg ttaatcccag acagatcgcg cttgaactca
    12541 ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat aatttctcgc taccgggagg
    12601 cgggccatga aatttatctt gatgattttg gtacgggata ttcaagttta agttatttac
    12661 aggatctgga tgtcgacatt ctgaagatcg ataaatcttt cgttgatgcg ctggaatata
    12721 aaaatgtcac gccgcatatc atcgaaatgg caaaaacact gaaactgaaa atggtagcgg
    12781 agggaatcga aaccagtaaa caagaagagt ggctacgcca gcatggcgtg cactacggtc
    12841 agggctggct ctacagcaag gcattaccga aagaagattt cttacgctgg gccgagcaac
    12901 atttgtgaat ttttgggctg acggcacgcc gcacagcccg tcacgctacc tattcgtcga
    12961 tgattgactc gaatccgcca tagatcattc gcttgccgtc aaacggcatg gactcgccga
    13021 actctttcat ccgtgggtcc gacatcatct tttgattagc agcgtcgcgg acctcttttg
    13081 aagggtattc aatccagcta aagacaacct cttcattctc ttccgctttc accgccatac
    13141 gaaaatcggt cactttgcca tccggtacat cgctggccca gcattcgaca atacgaagcg
    13201 cgccaaactc tttaaacaat ggcgcagcct tagcggccat ttcccgatag gcatcctttt
    13261 tatcggcagg aacggcaacc acaaaaccat caacatactt catcagacat acctccgaag
    13321 cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg gttaaagttt agccatgttg
    13381 gttttttttg acgaaacggt gggaggcaga aatgggtggg ggccctgcca gctacatccc
    13441 ggcacacgcg tcatctgcct tggctgcttc cttccggacc tgacctggta aacagagtag
    13501 cgttgcggga gaaccaacag gcccccattg agagcgttga gaaccaacgc gcaggcgcat
    13561 tatcactgct gccccggcta attgcaaccc atcgcgtgcc agatgctggt ttcttgcgca
    13621 atgcgacttc actcgggcgt ttttccagat tatgctgaaa aattacctca ggaagaaaag
    13681 atggaaaaag aagattcatt tccccaacgc gtctggcaaa tcgtcgccgc tattcccgaa
    13741 ggctatgtca ccacttacgg tgatgtggcg aaactggcgg gatcgccccg cgccgcgcgc
    13801 caggtgggcg gtgtgttaaa gcgtctccct gaaggcagca ccttaccctg gcaccgggtg
    13861 gttaatcgcc acggcacaat ttcgctaacc ggaccggatt tacagcgtca gcgacaggca
    13921 ttactggcag aaggtgtgat ggtatcggga agcgggcaaa tcgacttgca gcgttatcgc
    13981 tggaactact aaccctctcc gggcggagag ggttaacgtc gcttaatatt gcgtaggagc
    14041 tggaactgcc gaagacggat tcacctgagt tggtgaagtc gaaggtacgg tagtcgttgc
    14101 accaccgctg gcctgaaccg gcacggcggt ttgctgcacc ggcaccaatg tcaggtcggc
    14161 tttagttccg cccttgttga tcaccggctg aacggtatcg gtaataaata ccagtttgtc
    14221 attcacggta atcgccgcac tcaacagaat acgcgcgttc ggctgaacat ctgccggatt
    14281 aaatggcaga acaaagctga atggtgactg tttaccttcg gtacgcaccg ctttctgcgc
    14341 cagcactttt gacggtgcat cggctaacga cgcgtcagaa agtgtcacgg tcagcacagc
    14401 atcaggcggc agtgcgactt tctgccggat ccagacggta ccggagacat tcggttgctg
    14461 gatagcaggt tgttgtgttg ctgaaataga tgtatttgca gccggggctg gcgtctgaat
    14521 atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca accgctaaac cactggccat
    14581 gtgcacgagt ttcattcatt tctccttatt atcaatgcac cagcgggcta actttcctcg
    14641 ccggaagagt ggttaacaaa atagtaacgt caacaagtgt ggcacacatc acgcatttct
    14701 gcctgtaatt agcccgtaat tcagaccatt gcacccatcg gaccagtagc aaatttgcgt
    14761 tatactcaac tcactttggc ttgctgcggc agctttgtta ctggagagtt atatgagtca
    14821 ggcgctaaaa aatttactga cattgttaaa tctggaaaaa attgaggaag gactctttcg
    14881 cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc
    14941 cttgtatgct gcaaaagaga ccgtccctga agagcggctg gtacattcgt ttcacagcta
    15001 ctttcttcgc cctggcgata gtaagaagcc gattatttat gatgtcgaaa cgctgcgtga
    15061 cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac cgatttttta
    15121 tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa caatgccgtc
    15181 cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct
    15241 gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag tccgtccggt
    15301 ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg tgtggatccg
    15361 cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag tatctgctcg gttacgcttc
    15421 tgatcttaac ttcctgccgg tagctctaca gccgcacggc atcggttttc tcgaaccggg
    15481 gattcagatt gccaccattg accattccat gtggttccat cgcccgttta atttgaatga
    15541 atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct ttgtgcgcgg
    15601 tgagttttat acccaggacg gcgtgctggt tgcctcgacc gttcaggaag gggtgatgcg
    15661 taatcacaat taaaaaaaat agccggaggt aaaaccgtcc ggctattttt tgcagtgctt
    15721 gttacgcgtt ataggcattc tcgccgtgac tgttgacatc cagcccttct cgctcctgct
    15781 cttccggtac acgcagacca accgtcagat ccgccagttt gtagccaata aatgccacca
    15841 caccggacca gacgatcgta atggcgatgc tttccagctg taccagcaac tgatggccca
    15901 tcgtcacacc ttcagcgaag cccacgccac ccagcgaact ggcggcaaaa atcccagtca
    15961 tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag ggatcatcca
    16021 cccgcagcaa gcgtttgagc atagtaacgc cccacaagcc cgccagacca gctaccacgc
    16081 cgataatcaa cgcgccgcca accccaatgt agccacacgc tggcgtcacg ccgaccagac
    16141 cggcaatcgc gccagaacac gctcccagca gagaaggctt accacgcagc gcccattcac
    16201 cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca aatgccagtg
    16261 ccgcgatttc attcgccgtg cccgctgacc cggcgttaaa gccaaaccaa ccaatataga
    16321 gaatggcagt accggtgaag accattggca ggttgtgcgg tttaaacgcc tctttgccga
    16381 agcccacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgcg gcgttaatat
    16441 gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa ccaccgcccc
    16501 acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg aaaatcaaca
    16561 cagctgagaa gcggattcgt tccgccagcg ccccaacgat caagccgacg gtaatgcagg
    16621 caaacgagcc ctgaaacgcc acgtggatat actgataaat gctgcccatc accgccgtca
    16681 gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg ccctcaccaa
    16741 acgccagcga gtaaccgtaa accacccaga gaatacagac cagtgcaaac gtcaccgtca
    16801 cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa aacagggcaa
    16861 tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac gcattgtcgg
    16921 ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc agtgaagcaa
    16981 gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg ccagagatta
    17041 cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca attcagcgac
    17101 gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga tatcgatcac
    17161 ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca ggaagttgac
    17221 gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga aacctttcac
    17281 ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa cgtcttccag
    17341 cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt cagaattcgg
    17401 taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg aaaaacgaga
    17461 aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt gcacagtgcg
    17521 ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg attcttcttc
    17581 acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct gccagtagcg
    17641 tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc cacgatgaag
    17701 caccagaatg gtgtcggcat caacaatggt cgataagcgg tgagcaatca ccaccagcgt
    17761 ggtatgttca cgcacagccg ccagagcatg ttgaatcgcc tgttcagtac cggagtcaat
    17821 gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccaaca cgcgtgccag
    17881 tgccagcagt tgcttttgcc cgacggagag attattcccc tgctcgccca gcggcgtata
    17941 aataccatca ctcatgctgc gcgccagctc cgccagttgc acggtttcca gcgcctgcca
    18001 gacgcgttct tcggagatat cacgccccag cgtcacgttg gcgaggaagg tatccgccag
    18061 caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctgt gacttagcga
    18121 acttaatgga cgaccatcaa ggcgaatctc accttccgtt agcgggtaat agcccatcaa
    18181 taaactggcg agggtgcttt tgccactgcc ggtatgcccg accagtgcca caaagttacg
    18241 cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat aagcaaatga
    18301 cacgttatcg acttcgatgg tgccactctg taacgggcga tcatcattgc catattgctg
    18361 gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct gttgcagcat
    18421 cgcctgttgc gtggtcagtt cgattaatgg ttcgttaagt cgcccaagat agctgataaa
    18481 cgcatacagc acgcccactt caatggtgcc gctagcggag aagccaaaca gcatcaacaa
    18541 gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa aaccgtcgag
    18601 gcgcagggtt tgcatcctgg ccatatagtg tgaacgactg gcctccccca tacgttcgcc
    18661 aaatcgcgcc tgctgacgga actgctggat aacgctcatg ccattgatga tttcgttaaa
    18721 gccgtcgttg atatccgcta gataggcgcg cacacgacgg acaatcggcg tgctgtaacg
    18781 ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca ccagcgccat
    18841 tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg cgctacgcag
    18901 gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat tagtgacgcg
    18961 ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc gcaatgcggc
    19021 atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat taaacagcag
    19081 cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg ccgcagccag
    19141 ccctgcaacc attttcaacg gcaggttatt tttcgctacc atattgtcga taaaatagct
    19201 gataagcagc ggcccactga cttctgccgc tgccgcaacc cacatcatca ggaccgcaat
    19261 ccccagcggt ttacgccacg gcgaaccgta cgctaacaga cgcttgagag tcggccacag
    19321 ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggagcgtcg tcgagcgccg
    19381 cctccagttg ttgatagcga tacatatcgc gataccagcc gctttgttgc gccagctcat
    19441 catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca ctggcttccg
    19501 tcagtgccga aaggcgatgg gcactgatga ttaccgttct accctgtccc cactgacgca
    19561 ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca tcatcaagga
    19621 tgaggatttc cgcgttgact aataacgcac gagcaataga gatacgctgt ttttgcccgc
    19681 cggaaagcat cacgccgcgc tcgcccacct ctgtatcgta accttgcggt aaacgcaaaa
    19741 tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg gtggcattcg
    19801 ggcaacccaa cgcgatgttg ttcgctacgg tgtcggaaaa aaggaacggt gtctggctaa
    19861 ctaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga atatcatgaa
    19921 agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac agggtacttt
    19981 tgccggaacc agtcggcccg cagataccca gcatctgacc gggtttcagg gcgaaattga
    20041 cgttttccag cgcacgatgg tcagtctgcg gatacgtgaa ctggtgaata tttacatcaa
    20101 gttcgccacg cccttccggc acgggttcgc taccatcgtt caccaccggc gcttccgcca
    20161 gcatcgcacg aatacggctg tacgcagcac taccacgttc cacaatgtta aacatccatg
    20221 ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg gtgagctggc
    20281 ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc agcaagttcg
    20341 ccataccaat cgcgatatag attgtcgggt cgaaacgagc atcaatacgc gccacccgca
    20401 tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg cgatcttcca
    20461 gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg tcattaagac
    20521 tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg ttgcgcttaa
    20581 tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc cagctaattt
    20641 gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc agcgtcagca
    20701 ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga gccatgagat
    20761 cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga cggtaataat
    20821 cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg cgccagacat
    20881 aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac atcaggatca
    20941 gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc acttttggcg
    21001 gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccgaga tagcgacgcc
    21061 attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt gtcctgacct
    21121 gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt ccatcgcgaa
    21181 gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat aaaactcgtc
    21241 gtagcgtttc atgtcggcaa cctggacgcg catcagataa tcgtattcac cagccatgcg
    21301 ccagaacccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc aataccattc
    21361 gctgctgtga tgttgcgttt ttatcagcac aaaagcggtc aggccgaggc ctattttttc
    21421 cggatccagc agggcgactt tgccgataag gataccgtcg tcctccaggc gtttcagacg
    21481 cttccagcaa ggggtggttg tcagattaac ggcttcagcc agtgcctgca aagagagggt
    21541 gcaatcctgc tgcagtaagg ccagcagctt atggtcaatt ttatctaaca tagcccttcc
    21601 acagagaatt tttttctctt ttaattctat tttaaaggtg aaatcacgac aatttattct
    21661 gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa ccgggcaatt
    21721 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    21781 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    21841 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    21901 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    21961 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    22021 tcgaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    22081 tggatctgca agcgtgtaag atcgtcgtga ccgccacaga agcagatctt ggtgactctg
    22141 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    22201 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    22261 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    22321 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    22381 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    22441 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    22501 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    22561 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    22621 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    22681 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    22741 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    22801 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    22861 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    22921 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    22981 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    23041 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    23101 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    23161 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    23221 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    23281 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcatattg
    23341 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    23401 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    23461 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    23521 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    23581 aaaaagattt gggaaacagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    23641 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    23701 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    23761 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    23821 tactgccagg ctggactatt ccgtactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    23881 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    23941 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    24001 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    24061 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    24121 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    24181 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    24241 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    24301 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    24361 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    24421 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    24481 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    24541 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    24601 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    24661 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtccctggg
    24721 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    24781 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    24841 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    24901 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    24961 gatttagtcc gtaacgaaac gttgacctgc tataacggct ttaaaggcga cggttgcggt
    25021 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    25081 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    25141 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    25201 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    25261 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    25321 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    25381 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    25441 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    25501 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    25561 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    25621 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    25681 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    25741 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    25801 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    25861 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    25921 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    25981 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    26041 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    26101 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    26161 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    26221 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    26281 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    26341 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    26401 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    26461 gcttaagatt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    26521 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    26581 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    26641 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    26701 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    26761 aaccggcttg aagttcaact cttccggcag gttatctttg ctaaaccaac ccgtctgagt
    26821 ggctttaacg ccggcagctt gctctgcacc ggccagagac tccgtgtcgt tgcttgccgc
    26881 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    26941 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    27001 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt gccctttttc
    27061 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    27121 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    27181 accgccttta ttcagctcat caagtaccac tttcgcttca tcttcagttt tggtctggat
    27241 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    27301 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcatccagct tgatgtaact
    27361 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    27421 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    27481 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    27541 atcggtaccg gcaacaccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    27601 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    27661 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    27721 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    27781 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    27841 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    27901 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    27961 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    28021 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    28081 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    28141 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    28201 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    28261 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcagcagc gatggtgatc
    28321 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    28381 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    28441 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    28501 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    28561 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    28621 ttaataaggg caagcccgat ccgccatcta acttagcgag acaaaaaaag gctggcaagc
    28681 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    28741 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    28801 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    28861 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    28921 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    28981 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    29041 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    29101 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    29161 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    29221 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    29281 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    29341 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    29401 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    29461 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    29521 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    29581 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    29641 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    29701 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    29761 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    29821 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    29881 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    29941 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    30001 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    30061 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    30121 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    30181 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    30241 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    30301 tttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    30361 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    30421 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    30481 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    30541 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    30601 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    30661 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    30721 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    30781 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    30841 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    30901 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    30961 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    31021 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    31081 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    31141 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    31201 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    31261 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    31321 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acatcttcca
    31381 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    31441 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    31501 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
    31561 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgcgacaa
    31621 ccggcagacg accaataaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    31681 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    31741 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    31801 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    31861 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    31921 cctgctgtac gccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    31981 aaatcttgtc gatctcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    32041 cgcacttctg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    32101 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    32161 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    32221 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    32281 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    32341 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    32401 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    32461 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    32521 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    32581 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgccctg
    32641 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    32701 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    32761 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    32821 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    32881 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    32941 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    33001 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    33061 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    33121 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    33181 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    33241 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    33301 cgtctcctgg ataaaattga aaaaacctgc tgtaccgatt gtaaccttat ggacggatta
    33361 tcagctagtc cctgtgttat gggtacggca tcaccatatt tcaagcataa caatcttttg
    33421 ttgttacgct aacaccgaaa gaggcttttc gcacggttcc atgcaaaatt catgacaaaa
    33481 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    33541 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    33601 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    33661 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    33721 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    33781 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    33841 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    33901 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    33961 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    34021 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    34081 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    34141 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    34201 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    34261 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    34321 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    34381 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    34441 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    34501 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    34561 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    34621 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    34681 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    34741 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    34801 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    34861 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    34921 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    34981 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    35041 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    35101 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    35161 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    35221 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    35281 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    35341 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    35401 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    35461 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    35521 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    35581 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    35641 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    35701 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    35761 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    35821 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    35881 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    35941 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    36001 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    36061 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    36121 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    36181 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    36241 atcgtgccag ctacaatgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    36301 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    36361 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    36421 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    36481 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    36541 tggatgacgg tcgagaatat tgatctcaaa accattggtt tcttctctct ggtaggccag
    36601 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    36661 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    36721 atgggttttc tcgaacctgg cacccaactc cgctggatgg cggcactggc ggtggtgatc
    36781 gctttttgct cggcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    36841 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    36901 gtttccggcg gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac
    36961 tggttgatgg cggcgctgtt gatcccttgt attatcgcga cgttgcttgc accagaaccg
    37021 accgacacca ttcctgtgcc aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    37081 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    37141 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    37201 ggtgaagtag gcgtggttaa caaaacgctt ggcttactgg cgaccattgt gggcgcattg
    37261 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    37321 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    37381 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    37441 gtcgcgctgt taatgacgct gtgtaataag tcattttccg caactcaatt tgccctgctc
    37501 tcagcgcttt ccgctgtagg acgagtttat gtcggccccg tggcgggttg gtttgttgaa
    37561 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    37621 ttgctgctgg tttgccgcca gacgcttgaa tatacaggag taaatgacaa ctttatctcc
    37681 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    37741 ctgttggccg tgtggttact gctgttgacg atggacgcgc tggatttgac gcacttctct
    37801 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    37861 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    37921 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagt atcgcttaat
    37981 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tgacgcaatt tatgaattct
    38041 attttttctt acacgattca gctaatgagt ctttattttc ccccacccaa ttgtcaccat
    38101 aatgataatt atttgttaaa caattgtttt attccagatt ggttatacca attgcccgtc
    38161 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    38221 aacttgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta
    38281 atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag
    38341 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    38401 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    38461 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    38521 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta tcgtgcgagc
    38581 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    38641 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    38701 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    38761 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgctac cgtgaatgaa
    38821 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    38881 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    38941 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    39001 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    39061 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    39121 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    39181 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    39241 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    39301 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    39361 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    39421 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    39481 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    39541 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    39601 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    39661 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    39721 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    39781 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    39841 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    39901 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    39961 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    40021 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    40081 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    40141 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    40201 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    40261 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    40321 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    40381 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    40441 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    40501 actttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    40561 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    40621 ttcggctgct tcgcagggat gacctactgg tggcctaaag ccttcggttt caaactgaat
    40681 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    40741 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    40801 cagttccaca ccatgctgat gattgcagct agcggtgcag tactgattgc gctgggtatt
    40861 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
    40921 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    40981 tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag
    41041 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    41101 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    41161 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    41221 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    41281 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    41341 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    41401 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    41461 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    41521 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    41581 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    41641 tgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg
    41701 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    41761 cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct
    41821 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    41881 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    41941 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    42001 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    42061 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    42121 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    42181 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    42241 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    42301 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    42361 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    42421 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    42481 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    42541 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    42601 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    42661 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    42721 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    42781 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    42841 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    42901 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat
    42961 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    43021 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    43081 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    43141 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    43201 attgtctgag atcagagata cggctaataa ccgtatgtct tgacagagtg agcagatgaa
    43261 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    43321 attcagttaa tcaaagaggt cataatgcct ggaaaaacag ccactctacc cgatgtcgat
    43381 aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    43441 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg
    43501 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    43561 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    43621 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    43681 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    43741 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    43801 tgcggctaaa gcacttattg acagacaaag tggtgaactg atcggcatct gtacttttac
    43861 ggcatattcg ctggaaaaac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    43921 aattggtgtt gtcagattag tcatgttggg ggtagcacgg aagtatcaaa agcggggctt
    43981 tggtcaggac ctactatgtg atttttttga acatgtaaaa ataattcacc aggcattacc
    44041 aattaaaggg gtttatcttg atgctgaccc tgccgccatt aatttttatg ctcgtctcgg
    44101 ctttgttcag ctttcagcga caccaaatgc ttttggtgct gtacctatgt ttttggcgat
    44161 tcagcatatt ctcgcggctt agattattat cgccacccgc cagggcaagt tttgcctcag
    44221 ccgggggctg agcagcgtta ccacttttgt tcaattgccc ccgctttcag caactccaca
    44281 tccttgccgt taatggttgc cgttgcggca acgattgtgg tccttacagt tgcacaagat
    44341 tttcgctcaa attccggttt ataaagaata agagacgaat tactggtaaa tgaaccaaat
    44401 aggtaatcag taaagctaat attcagccgg tttaaaccga aaggcgcgaa gccaaccgtt
    44461 tggttaatct ccttaccgga tgggcgacga atgctgaatg tcacgcgaag attggccact
    44521 tcgcctaatt tattggtggt ggcgaagagg ctatccatgc aggaaaaacc tttatctaaa
    44581 aaattatctc gcatcccaga acgggtaacc gtaatcacgg gtaacgtatc gtcaaccgtt
    44641 tttctgtcaa tcatgtcttt tcgacaagta gccagaaagt cttctgggcc accataaatg
    44701 tggacacctt tatcaagata ttccttcgct ttcttacaat ctacctcaac atactgacca
    44761 ttggcgtaca taatgcccaa cctggacctt gctaaattca caccgtgctg cgcggcctga
    44821 ttaaatagtg catacgcttt ggcataatcc acccgcacct gctcaccgtc gtagtagata
    44881 ccccccagcg ctaacggagc ttctctgtca ccttgtttag ccactaactc gaaccattta
    44941 atcgcttcct gaacatcagg tttggtatca taacgaggct gataatattc ccagcctaac
    45001 aggctctgcg cgtcgatatc tcccgattca gccattttat gcagtgtatt tataacatcg
    45061 ggagcaggtt taccaccgta tatgctgtca tacaattcat gcgctttctc gctggccacc
    45121 acgcttggtg aaactatcag cattacagaa aataatattc ttttagttaa catgtatacc
    45181 tttatgtcct cactgtcata tatttccata ataattaacc accaaatatc catttaatga
    45241 ataaatacag tgccataaag gtccaagtaa taagcacaca tttagcctga aacattatca
    45301 tccatgtcac caaatcgttt atttagtgag ataaaactag catattcgta aga
//
LOCUS       DAZFDP010000020        45166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFDP010000020 DAZFDP010000000
VERSION     DAZFDP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..626
                     /gene="yadN"
                     /locus_tag="ACHJ1G_001234"
     CDS             42..626
                     /gene="yadN"
                     /locus_tag="ACHJ1G_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4763124.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            731..1471
                     /gene="yadV"
                     /locus_tag="ACHJ1G_001235"
     CDS             731..1471
                     /gene="yadV"
                     /locus_tag="ACHJ1G_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4763125.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1506..4106
                     /gene="htrE"
                     /locus_tag="ACHJ1G_001236"
     CDS             1506..4106
                     /gene="htrE"
                     /locus_tag="ACHJ1G_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO4763126.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4123..4692
                     /gene="yadM"
                     /locus_tag="ACHJ1G_001237"
     CDS             4123..4692
                     /gene="yadM"
                     /locus_tag="ACHJ1G_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4763127.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4707..5309
                     /gene="yadL"
                     /locus_tag="ACHJ1G_001238"
     CDS             4707..5309
                     /gene="yadL"
                     /locus_tag="ACHJ1G_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO4763128.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5336..5932
                     /locus_tag="ACHJ1G_001239"
     CDS             5336..5932
                     /locus_tag="ACHJ1G_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4763129.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5916..7259
                     /locus_tag="ACHJ1G_001240"
                     /pseudo
     CDS             5916..7259
                     /locus_tag="ACHJ1G_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7372..8166
                     /gene="panB"
                     /locus_tag="ACHJ1G_001241"
     CDS             7372..8166
                     /gene="panB"
                     /locus_tag="ACHJ1G_001241"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4763130.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8178..9029
                     /gene="panC"
                     /locus_tag="ACHJ1G_001242"
     CDS             8178..9029
                     /gene="panC"
                     /locus_tag="ACHJ1G_001242"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4763131.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9103..10005)
                     /gene="rpnC"
                     /locus_tag="ACHJ1G_001243"
     CDS             complement(9103..10005)
                     /gene="rpnC"
                     /locus_tag="ACHJ1G_001243"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4763132.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10279..10659
                     /gene="panD"
                     /locus_tag="ACHJ1G_001244"
     CDS             10279..10659
                     /gene="panD"
                     /locus_tag="ACHJ1G_001244"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4763133.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10663..11892)
                     /gene="yadE"
                     /locus_tag="ACHJ1G_001245"
     CDS             complement(10663..11892)
                     /gene="yadE"
                     /locus_tag="ACHJ1G_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4763134.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11956..12396)
                     /gene="yadI"
                     /locus_tag="ACHJ1G_001246"
     CDS             complement(11956..12396)
                     /gene="yadI"
                     /locus_tag="ACHJ1G_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4763135.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12501..13271)
                     /gene="yadH"
                     /locus_tag="ACHJ1G_001247"
     CDS             complement(12501..13271)
                     /gene="yadH"
                     /locus_tag="ACHJ1G_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4763136.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13268..14194)
                     /gene="yadG"
                     /locus_tag="ACHJ1G_001248"
     CDS             complement(13268..14194)
                     /gene="yadG"
                     /locus_tag="ACHJ1G_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4763137.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14303..14965
                     /gene="can"
                     /locus_tag="ACHJ1G_001249"
     CDS             14303..14965
                     /gene="can"
                     /locus_tag="ACHJ1G_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4763138.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15006..15542)
                     /gene="hpt"
                     /locus_tag="ACHJ1G_001250"
     CDS             complement(15006..15542)
                     /gene="hpt"
                     /locus_tag="ACHJ1G_001250"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4763139.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15748..18138
                     /gene="gcd"
                     /locus_tag="ACHJ1G_001251"
     CDS             15748..18138
                     /gene="gcd"
                     /locus_tag="ACHJ1G_001251"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4763140.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18185..19735)
                     /gene="cueO"
                     /locus_tag="ACHJ1G_001252"
     CDS             complement(18185..19735)
                     /gene="cueO"
                     /locus_tag="ACHJ1G_001252"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4763141.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19901..20248
                     /gene="yacC"
                     /locus_tag="ACHJ1G_001253"
     CDS             19901..20248
                     /gene="yacC"
                     /locus_tag="ACHJ1G_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4763142.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20354..21220
                     /gene="speE"
                     /locus_tag="ACHJ1G_001254"
     CDS             20354..21220
                     /gene="speE"
                     /locus_tag="ACHJ1G_001254"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4763143.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21236..22030
                     /gene="speD"
                     /locus_tag="ACHJ1G_001255"
     CDS             21236..22030
                     /gene="speD"
                     /locus_tag="ACHJ1G_001255"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4763144.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22068..22430)
                     /gene="yacL"
                     /locus_tag="ACHJ1G_001256"
     CDS             complement(22068..22430)
                     /gene="yacL"
                     /locus_tag="ACHJ1G_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4763145.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22605..25202)
                     /gene="acnB"
                     /locus_tag="ACHJ1G_001257"
     CDS             complement(22605..25202)
                     /gene="acnB"
                     /locus_tag="ACHJ1G_001257"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4763146.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25557..27314
                     /gene="yacH"
                     /locus_tag="ACHJ1G_001258"
     CDS             25557..27314
                     /gene="yacH"
                     /locus_tag="ACHJ1G_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4763147.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27385..28809)
                     /gene="lpdA"
                     /locus_tag="ACHJ1G_001259"
     CDS             complement(27385..28809)
                     /gene="lpdA"
                     /locus_tag="ACHJ1G_001259"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4763148.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29134..31026)
                     /gene="aceF"
                     /locus_tag="ACHJ1G_001260"
     CDS             complement(29134..31026)
                     /gene="aceF"
                     /locus_tag="ACHJ1G_001260"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4763149.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31041..33704)
                     /gene="aceE"
                     /locus_tag="ACHJ1G_001261"
     CDS             complement(31041..33704)
                     /gene="aceE"
                     /locus_tag="ACHJ1G_001261"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4763150.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33865..34629)
                     /gene="pdhR"
                     /locus_tag="ACHJ1G_001262"
     CDS             complement(33865..34629)
                     /gene="pdhR"
                     /locus_tag="ACHJ1G_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4763151.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35170..36543
                     /gene="aroP"
                     /locus_tag="ACHJ1G_001263"
     CDS             35170..36543
                     /gene="aroP"
                     /locus_tag="ACHJ1G_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4763152.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36586..37440)
                     /gene="ampE"
                     /locus_tag="ACHJ1G_001264"
     CDS             complement(36586..37440)
                     /gene="ampE"
                     /locus_tag="ACHJ1G_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4763153.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37437..37988)
                     /gene="ampD"
                     /locus_tag="ACHJ1G_001265"
     CDS             complement(37437..37988)
                     /gene="ampD"
                     /locus_tag="ACHJ1G_001265"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4763154.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38076..38969
                     /gene="nadC"
                     /locus_tag="ACHJ1G_001266"
     CDS             38076..38969
                     /gene="nadC"
                     /locus_tag="ACHJ1G_001266"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4763155.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39172..39612
                     /gene="ppdD"
                     /locus_tag="ACHJ1G_001267"
     CDS             39172..39612
                     /gene="ppdD"
                     /locus_tag="ACHJ1G_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4763156.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39622..41007
                     /gene="gspE"
                     /locus_tag="ACHJ1G_001268"
     CDS             39622..41007
                     /gene="gspE"
                     /locus_tag="ACHJ1G_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4763157.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            40997..42199
                     /gene="hofC"
                     /locus_tag="ACHJ1G_001269"
     CDS             40997..42199
                     /gene="hofC"
                     /locus_tag="ACHJ1G_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4763158.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42234..43277)
                     /gene="guaC"
                     /locus_tag="ACHJ1G_001270"
     CDS             complement(42234..43277)
                     /gene="guaC"
                     /locus_tag="ACHJ1G_001270"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4763159.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43433..43477)
                     /locus_tag="ACHJ1G_001271"
     CDS             complement(43433..43477)
                     /locus_tag="ACHJ1G_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763160.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43502..44122
                     /gene="coaE"
                     /locus_tag="ACHJ1G_001272"
     CDS             43502..44122
                     /gene="coaE"
                     /locus_tag="ACHJ1G_001272"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4763161.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44122..44865
                     /gene="zapD"
                     /locus_tag="ACHJ1G_001273"
     CDS             44122..44865
                     /gene="zapD"
                     /locus_tag="ACHJ1G_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4763162.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            44875..45072
                     /gene="yacG"
                     /locus_tag="ACHJ1G_001274"
     CDS             44875..45072
                     /gene="yacG"
                     /locus_tag="ACHJ1G_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4763163.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT        11291 a        11733 c        11012 g        11130 t
ORIGIN      
        1 aaccagtagt ctgactaatg gaattttatt caaggatttt tatgtctaaa aaattaggtt
       61 ttgccctgtc tggtcttatg ctggcaatgg ttgcaggtac tgcttctgct gatatggatg
      121 gtggtcagtt aaatatcagt ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg
      181 gcggtaataa agatggtctg atcctgctgc aaactgcaac tgttggtgaa atcgatgctg
      241 gcgtgctgaa tgacaccgtt ggcgctaaag ccaaaccttt cagcatcacc gttgactgca
      301 gcaaagcaaa tccgaatcca ggtagtacag ctaaaatgac cttcggttct gttttctttg
      361 gtaatagcaa aggaacgctg aacaacgaca tgtctattaa caacccgtct gacggcgtga
      421 atatcgctct gcacaatatt gatggttcca ctatcaaaca ggttcaaatt aacaaccctg
      481 gcgatgtgta tactaaagcc ctggatgcaa cgacaaaatc tgctgtttat gattttaaag
      541 cgtcttacgt tcgtgccgtc gcagaccaaa cagcaactgc tggttatgta aaaactaaca
      601 ctgcatacac cattacttat cagtaagtat tcaccaatta aattgattga atgaatatac
      661 agggaatagt gttaataatt tctattttta tattattccc tgttttaatt aactctgtca
      721 gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg taacctgtat
      781 ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg taatatataa
      841 aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc cgttgcttgt
      901 ccagagttgg ttagatactg gcgatgacaa cgctgagcct ggcagtatta cagtcccttt
      961 tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat
     1021 gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt ttaacgtact
     1081 ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc
     1141 atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa atccctctga
     1201 agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt
     1261 gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag ctagcggtaa
     1321 acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca tgaaagtcac
     1381 tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta atgattttgg
     1441 tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc cgcaggaagc
     1501 ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt atcgctacgt
     1561 tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa tatgaccata
     1621 ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt gaaggtaacc
     1681 ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct attattaacc
     1741 aaagcattac atttattgaa attgaaggaa aaaaaaacgc acaagcctgt atcacactta
     1801 agaatttatt gcagtttcat ataaattctc ccgatataaa taacgaaaaa gccgttctgc
     1861 ttgccaggga tgaaacactc ggcaattgcc ttaatttgac ggaaattatc cctcaggctt
     1921 ctgttcgtta tgacgttaac gagcaacgtc tggatataga cgttcctcag gcatgggtaa
     1981 tgaaaaatta ccaaaactat gttgatccat cgttatggga aaatggcatt aatgcggcca
     2041 tgttgtcata caacctcaac ggatatcata gtgaaactcc tggtcgaaga aatgacagca
     2101 tttatgctgc ttttaacggt gggatgaatt taggtgcatg gcgactgcgt gcctcaggca
     2161 actacaactg gatgaccgat tctggcagta attatgattt taagaatcgg tatatacagc
     2221 gtgatatcgc ctcgctgcgt tctcaactca ttcttggtga gtcttataca acgggcgaaa
     2281 cctttgattc tgtcagtatc cgcggcattc gtttatacag cgacagccgc atgttgcctc
     2341 cgacattagc gagttttgcg cctatcattc atggcgttgc taataccaac gccaaagtca
     2401 ctattacgca aggtggctat aagatttatg aaacgactgt gccgccaggc gctttcgtca
     2461 ttgatgatct gagtccgtca ggttacggca gcgatcttat tgttactgtc gaagaatccg
     2521 atggctcaaa gcggacattc tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg
     2581 gcgttggacg ttgggatatt agcggcggcc aggtcttaaa agatgatatt caggatgagc
     2641 ctaatttatt tcaggccagc tactactacg gtctgaataa ctatctaacg ggttatactg
     2701 gtattcagat taccgataat aactataccg ctggtttgtt aggtcttggt ctgaatactt
     2761 cagtcggtgc attttctttc gatgtgactc actccaatgt tcgtataccg gatgataaaa
     2821 catatcaggg acaaagttat cgtgtttcct ggaacaagtt attcgaagaa acaagtactt
     2881 cactgaatat cgcggcctat cgctattcga cacagaatta ccttggtctt aatgatgcac
     2941 taactctaat tgatgaagtg aaacatcccg aacaagatct tgaaccgaaa tccatgcgta
     3001 attactcgcg catgaaaaat caggttacgg tcagtattaa ccaaccgcta aaatttgaga
     3061 aaaaagatta cggttcattt tatctttccg gaagttggtc cgattactgg gcttccggac
     3121 aaaatcgtag caattactct attggctaca gtaacagtgc atcctggggc agctacagtg
     3181 tcagtgccca gcgttcatgg aatgaagacg gcgacactga cgatagcgtt tatctcagtt
     3241 tcaccattcc tattgaaaaa ttacttggca ctgaacaacg gacttcaggt ttccagagta
     3301 ttgatactca aatgagcagt gactttaagg gtaataacca actcaacgtc agtagcagtg
     3361 gctatagtga taacgctcgc gtcagttata gcgtaaatac tggctatacg atgaataaag
     3421 ccagcaaaga tttgagttat gttgggggtt atgccagcta tgaatcaccg tggggaaccc
     3481 tggcaggttc agtttctgca aatagcgata acagtcgtca agtgtctctc agcaccgacg
     3541 gtggttttgt attgcatagc ggcggcctga ccttcagtaa tgacagtttt agcgactccg
     3601 atacactggc ggtagttcag gctccaggtg ctcaaggagc gcgaataaat tatggcaaca
     3661 gtactatcga tcgatggggt tatggtgtca ccagcgctct ttctccttat catgaaaacc
     3721 gtatcgcgct ggatatcaac gatcttgaga acgatgttga attaaaaagt accagtgcag
     3781 tagctgtacc gcgtcagggt tcagtcgtct ttgctgattt tgaaaccgtg caagggcaat
     3841 cagccattat gaacatcaca cgaagtgatg gtaaaaatat tccatttgct gcagatattt
     3901 atgatgagca aggcaatgtc attggtaatg ttggacaggg tggacaagca tttgttcgtg
     3961 gtattgagca gcagggaaat atcagcatta aatggctcga agaaagtaaa cccgtaagtt
     4021 gtcttgcgca ttatcaacaa agttcagaag cagaaaaaat agcgcaatct attattctga
     4081 atggaattcg gtgtcagatt cagtaactac aagggacgtc aaatgataaa aataacgcca
     4141 cataaaatag cgatactgat gggaatatta ttatcaccat cagtatttgc aacggatatt
     4201 aatgtagagt ttacagccac tgtcaaagcg acaacctgta acatcacact tactggtaat
     4261 aacgtcacga atgatggcaa taataactac acattgagaa tccctaagat gggtctggat
     4321 aagatcgcga ataaaacgac agaatctcag gctgatttta aactggttgc tagtgggtgc
     4381 agcagtggca tcagttggat tgataccact ctgagcggaa atgcatcatc aagctcacct
     4441 aagcttatta taccgcagtc tggtgattca tcttcgacga caagtaatat cggtatgggt
     4501 ttcaaaaaac ggactactga tgatgccact ttccttaaac ctaacagtgc ggaaaagata
     4561 cgctggagca cagacgagat gcagcccgat aagggtcttg aaatgaccgt tgcgctacgt
     4621 gaaacagatg cagggcaagg cgtaccgggg aattttcgtg cactggcaac gtttaatttc
     4681 atctatcaat aagaaggata attaagatga catttaaaaa tttacgttat ggattatcca
     4741 gcagtgtcgt tttggctgcc tcattgttca gcgtaccctc ttatgcggca acagacagca
     4801 ttgggctgac cgtgattact accgtagaaa taggtacttg taccgctaca ttagtaaatg
     4861 actctgatca ggacatttct gttgttgact ttggcgatgt atatatttct gaaatcaatg
     4921 ccaagaccaa agttaaaaca ttcaaactca aattcaaaga ctgtgcgggt atccccaata
     4981 aaaaagcaca aataaaatta accaagcgag ccacatgcga aggtactgct aatgacggtg
     5041 cggggttcgc aaatggatcc acagccgcag ataaagcaag tgccgtcgcc gttgaagtct
     5101 ggagcaccga aactccggca acagggagcg caacacaatt tagttgtgtg acaccagcat
     5161 ctcaagaggt cactatctcc aatgcagcca acgcggttgt ttattatccg atgagtgctc
     5221 gactggtcgt cgaaaaaaat aaaaccgtaa gcaatgtcac tgcgggtaaa ttttctgcac
     5281 ctgccacatt tacagtaacc tataactaac agactgacag atccaggaac tatatatgca
     5341 tcccactcaa cgtaagctga tgaagagaat aattctgttt ctgtcactac agtttcccat
     5401 tacctatcca gccatcgctg ggcaggatat tgaccttgtt gccaatgtaa aaaacagcac
     5461 ttgcaaaagc ggaatcagta accagggtaa tattgatctt ggcgtcgttg gggtgggata
     5521 tttttcagat aatgttactc ctgaaagcta tcaaccaggt ggaaaagagt tcactgtcac
     5581 agtatcagac tgtgcattac agggaactgg cgatgtgctt aatcagttac atattgattt
     5641 tagagccctt agcggtgtca tggctgcagg ttcaaggcaa atatttgcca atgaagtcgc
     5701 aaccggggct aaaaatgtcg gtgtcgttct cttttccatt caggacccga ccaatatatt
     5761 caatgttatt agttcagcag gaaattctcg ttccgtgtat ccagtaatga cttctgccct
     5821 gcataattct tcatggaaat tttatgccag aatgcaaaaa atcgatcccg cgctggatgt
     5881 catatctggc caggtaatga gtcatatatt agttgatgtg tactacgaat aatagatcaa
     5941 aacaaataac tgcgactgtt acagcagtag atggtggagt gataatgaag aaattcttca
     6001 gacattttct ttttttaatt ctgtgcttga gttgttatac cgcgagtgct ggcactgatg
     6061 ataacgtagg atatattgtt gggaatagtt acggcgttgg tccgtcagat caaaaatgga
     6121 gagaaacggg gccgaatggc gatgctactg tcatattcag atatgcaaca agtacaaata
     6181 atttagtttt ctacaaaccg acgcagcttg gacctacagg tgtaaaatta cagtggagtc
     6241 agttagatac cgcttctggt ggtggttttc tttattgcaa cagaagtgat agcacaagtg
     6301 gtagcgcaat gcgtattgaa aatgcaatgg ttgactcagg taaaatgtat ggctcccata
     6361 aattatttaa tacatcagtt cctggtttgt attacacatt attaatttca aacatgtggt
     6421 cagcttacgg taccgtaact aacgttagtt cacctgggat atacattggt gactctgcag
     6481 aacaatattt ttcgtggtat aatccaagcg aagacgtgtt atattagagt tgcaataatg
     6541 cgaatagcac ccgtaaatac tgggctgtag gtggtattta tcagaccctt acaattgaat
     6601 tctatacaga tacaaacttt gatccaacag taactcaaca gatcaaatta tccagctcat
     6661 caaattatct gtattcattt aaagcctatg gcgcaggcca aggtataaat gagcatagtt
     6721 attttatcaa aatcgatttc gatctgctca atgtcaagtt gactaatccc acttgtttta
     6781 ccgctatgct tagtggaact tctgtgactg gttctacggt aaaaatgggt gaatatagtg
     6841 cagaacaaat cagaaacggt gccacaccgg ttccttttga tatttcactt caaaactgcg
     6901 ttcgtgtgac taatattgag acaaaattag tttcaacaaa ggttggtact gaaaacgggc
     6961 aactccttgg taatactctc acaggtaatg acgcagcaaa aggagtcggt gtactcatag
     7021 aaggtttagc aactagtaaa aatcctctaa tgacattgaa acctaatgat tcaaattctg
     7081 tttataaaga ttacgacccg agaggcaaag acgatacaac aggaggagtt tacccggatc
     7141 aagatacagg tataacatac cctctccatt tccaggccac gctacaacag gatggaacta
     7201 taccaataga agctggtgag tttaaagcca ccagtacttt ccaggtaacc tacccttaat
     7261 aagtccaccg caccgccatc atctggcggt gcagtaattg ataaatcatt gcccccacca
     7321 atgtgactga taaaatgcca ggcaacacga catcatttat caggatacgt tatgaaacca
     7381 accaccattg cttcacttca gaaatgtaag caggataaaa agcgtttcgc gaccatcacc
     7441 gcttacgact acagcttcgc gaaactattt gctgatgaag gacttaacgt catgctggta
     7501 ggcgactcgc tgggcatgac ggttcagggt cacgactcca ccctgccggt gaccgttgct
     7561 gatatcgcct accacactgc cgccgtacgt cgcggcgcgc caaactgcct gctgctggct
     7621 gatctgccgt ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt
     7681 atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta
     7741 aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact taggtttaac cccacagtca
     7801 gtgaatattt tcggtggcta caaagttcag gggcgcggcg atgaagcggg cgatcaactg
     7861 ctcagcgatg cattagcctt agaagccgct ggggcacagc tgctggtgct ggaatgcgtg
     7921 ccggttgaac tggcaaaacg tattaccgat gcactggcga tcccggttat tggcattggc
     7981 gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg acgccttcgg cattaccggc
     8041 ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct
     8101 gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc
     8161 cattaaggag tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc
     8221 gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg
     8281 atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta
     8341 ttttcgttaa cccaatgcag tttgaccgcc cggaagatct ggctcgttat ccacggacct
     8401 tgcaagagga ctgcgaaaag ctaaacaaac gtaaagtgga tttagttttc gccccttcgg
     8461 taaaagagat ctacccgaac ggtactgaaa cccacactta cgttgacgtt cctggccttt
     8521 cgaccatgct ggaaggtgcc agccgtccgg gacattttcg cggcgtttcg actattgtca
     8581 gcaagctgtt caacctggtc cagccggaca tcgcctgctt cggtgaaaag gactttcagc
     8641 aactggcgct aatccgcaaa atggttgccg atatgggctt cgatattgaa attgtcggcg
     8701 tcccgattat acgcgccaaa gatggccttg cgctcagttc ccgtaatggt tatctgacgg
     8761 cggaacaacg caaaattgcg cccggtctgt acaaagtttt aagttcaatt gccgacaaat
     8821 tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat tgcggggcaa gaactgaatg
     8881 aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc cgacacattg ctggaagttt
     8941 ctgaaaccag caaacgggca gtaattctgg tagccgcctg gcttggcgat gctcgcctga
     9001 tcgacaacaa aatggtcgag ctggcgtaat acttaactgg cgctacggct gatggcgcca
     9061 gttattaatt taccccacgt catctgtcat aacacaggta acttaaataa ggttaattac
     9121 cttatcaatc tcagcaagag gtaaatttgc catctctgca acgtcttccc gagacattcc
     9181 tttacttaga agacgcaggg cgaattcttg cctttcctcc tgtcttccct gctgaatccc
     9241 cttctcaatc cctttctctt caaaccactg cgccagcgtc atcatagact cccctcccgt
     9301 ttccctgtct ctcagcacac cataaaacaa atccgcttgt tcagtatgac cgcgttgcag
     9361 catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt caatcagcgt
     9421 gaccagttgc tccagcaata acattaagtc gcgctggcga atatgttttt gcagtagttc
     9481 gagaatcgca atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg tgatatccac
     9541 cagcgggaaa ggactgttat agacgcgtcg tgccagctcc ggcgagtaaa atatatcaaa
     9601 ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagta tcggcaccac
     9661 cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcagcaa tagaataacg
     9721 catcatgcga aaggccattt tcttatccgg cttgctttgg tgttcaatca caacatgcag
     9781 ataaccggga ttgccctgca tttgcacgga atagagcacg tccgtgctgt gtcctttcag
     9841 gctctcttca atgaaactcc ccgactctaa atgaagcgtg ttgaggtcac aaagttctcg
     9901 taattccact ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcgt gcattaaaaa
     9961 ttgtttaaac actgcatcat gcggtgtggt actcggtgca tccatcttcc ctgccttccg
    10021 tgttgtcttg cgtgaaggca gtatccagtc aaggcgtgcc atcgtcagcc acctctttag
    10081 tgtttaccga gcagcgttca gagaaatttc aattggttac agccgttaca tggcacgctt
    10141 cggcacggta cgcaaactgc tgatgtaaat gccgtcagat gagtagacac taaacaaaaa
    10201 tcgggcaata ctgcgtgaga atttcctcaa agcaggcatc gcctgcttcg ttaacgacag
    10261 ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca ccgcgtgaaa
    10321 gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgaccagga ttttcttgac
    10381 gcagccggta ttctcgaaaa cgaagccatt gatatctgga atgtcaccaa cggcaagcgt
    10441 ttctccactt atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt taacggtgcg
    10501 gcggcccact gcgccagtgt cggcgatatt gtcatcatcg ccagcttcgt taccatgcca
    10561 gatgaagaag ctcgcacctg gcgacccaac gtcgcctatt ttgaaggcga caatgaaatg
    10621 aaacgtaccg cgaaagcgat tccggtacag gttgcttgat tgttatccct gcggctggtt
    10681 actcaccagc cgcgacatcg tctccagcga atccgttctt aagatataaa gtcgttttag
    10741 taacaacgga ttatcccccg gttttacttt gcctttcatg gttgtcaccg ccaggtgaaa
    10801 tccggcatcg tttgctgcct tcacggcttt gtcattaaat ccgccaaacg gatacgaaag
    10861 ataccagaca tgcggattaa attgcgccag agcgcggcgt gaacgtgcaa aatcaaacag
    10921 aatattgtgc tcactacggc tcagtaatat gggtcggcga tagccatcca cccgatgcaa
    10981 aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt tcgttaagct cagaaacgct
    11041 cataaattgc agcgattttg ggttccactt ctgcgggtga cgtttgatgc gcgaggtaac
    11101 aacaaacgcc gtcgccttca tgccatattg tttcaataca ggatacgcat agcggctcac
    11161 cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat tgatcttatt
    11221 cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt cgcgcagcca
    11281 ggccatttgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc gaaaacgggt
    11341 gttttcttca tcgcgcagaa tatggtgata ggttagcacc ggcaggccat tatcgggttg
    11401 ggcatccagc gcgctgatat aggcgagtcg atcgccaata cggatctgat accaggtttg
    11461 atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg ccagtacccc
    11521 aaatggcgca ctgcccgcac tcggcgcgtt atagaccggc gtatctttcc aggtaattaa
    11581 gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt tttgtcgccc
    11641 ctgaactggc tcgagatgcc ctttatcgat aaacccttta ccaaagccaa aattaaatgc
    11701 gtaataactt gcggcagtgg gctccacggc aatgatttgc ccggcccgaa tattccccac
    11761 ggtcaccatc ttgtcgccaa tttgcgccca gatcgcagca ttttcgatgg tttgcatata
    11821 acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga gaataacagc
    11881 ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta gcaaaagcgc
    11941 tataagaatc gtgggttaat actcatacaa attatgtttg aggacgaaag gcggattttt
    12001 ttgttgttgg tgccaaagac tactcacctc cggcccaccc agttcgacaa tacgctcgcg
    12061 aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta acggtaacgt
    12121 gacaccagaa atcacttcac aacgggagtg tttatggctt aataatgaag ccacgcggta
    12181 cggtggcgct ccggctaaat cggttaagaa gatgacaccc tcaccagagt ccgcttcatg
    12241 cagagcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc agaaattcac
    12301 ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta tctcttgcgc
    12361 ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta gccgaaaatc
    12421 agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc tcccccgaat
    12481 ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat cagcgaccaa
    12541 cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt gaccagcggc
    12601 acatcattta taccgaggaa gccgtagcgg aaaccactga tcatataaac gattgggttc
    12661 aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa caccccgccc
    12721 aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg
    12781 gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgccgtgag caccagggtt
    12841 aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga aattgccgtc
    12901 accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc ggcaataatg
    12961 acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa cttggcacca
    13021 aaaaatgatg acgcgacgtt agcgtaggca ttggtgatca ccgacatcat gatcagcccc
    13081 ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga acctatcagg
    13141 ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag cgtctgcacc
    13201 cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc cacccagtaa
    13261 agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac agctcttcca
    13321 gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt aactgcgtaa
    13381 atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca
    13441 gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga tcgagaataa
    13501 aggtttccga tttcagctta gccagcagcg ccttcatcga ggtattttcc accagctcac
    13561 cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg tagtgtgtgg
    13621 tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac attgagcggc
    13681 gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta ggttcatgca
    13741 ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata cgcgcacgtt
    13801 cgttgcgttt tccccataga tcgagctgct taagatattt ttcgctgcgg atgtacgcct
    13861 ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc acggtttcaa
    13921 acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg ttcacgacat
    13981 ccttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt accagagagc
    14041 tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc gcataaaaat
    14101 cacccgcttc gacctgcaaa tctatcccac gaagcgcctg aacgccgcct ggataggttt
    14161 ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct tacgttctta
    14221 cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa cgtaattatt
    14281 tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca acaatgcact
    14341 atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg cacaagcgca
    14401 aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag aacgtttaac
    14461 cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg tcattcacac
    14521 tgacctgaac tgcctttccg tggttcagta tgcagtggat gtactcgaag ttgaacacat
    14581 tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa acccggaact
    14641 ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac atagctcatt
    14701 gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga acgtcatgga
    14761 acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac gcgggcagaa
    14821 agttaccatt cacggctggg cctacggcat tcacgacggc ttgctgcgtg atctggatgt
    14881 taccgccacc aaccgcgaaa cccttgagca acgttaccgt cacgggattt ccaacctcaa
    14941 gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat ccggcaactt
    15001 cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg gtaacgctgt
    15061 gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc gataaattct
    15121 accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat cgccagcgac
    15181 ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc cgagtcgatg
    15241 atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc tttgaggatt
    15301 ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat aaagtcgact
    15361 tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga gccacgcagc
    15421 agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat ctgacgaccc
    15481 agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac agtatgtttc
    15541 atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc
    15601 aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa tctgttaata
    15661 aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg ctactaaaat
    15721 attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc gcgacgatta
    15781 ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact cattggcgga
    15841 ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct tgtgatgctc
    15901 ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctata cgccgccctg
    15961 ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg ggcgctgact
    16021 ccgcgcagcg atattctggt cttcttcggc atctggctga tcctgccgtt tgtctggcgt
    16081 cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact gctgattagc
    16141 ggtagtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg caccttaagc
    16201 gccgatgcca cacctgcgga agctatctca cccgttgccg atcaggactg gcctgcctat
    16261 ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc cgataacgtc
    16321 cataatctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc gaacgatccg
    16381 ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta cctgtgtacc
    16441 gctcaccagc gcctgtttgc gctcgatgcc gccagcggca aagagaaatg gcattacgat
    16501 cctgagctga aaactaacga gtctttccag cacgtaacct gccgtggcgt ctcttatcat
    16561 gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt
    16621 cttccggtca atgatggtcg actgattgcg attaacgctg aaaacggcaa actgtgcgaa
    16681 accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac caaaccgggt
    16741 ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat ggcaggttca
    16801 gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac
    16861 accggggagc tgctgtgggc ttttgatccc ggcgcgaaag atccgaacgc aatcccgtct
    16921 gacgaacaca cctttacctt taactcgcca aactcgtggg cgccagcggc ctatgacgcg
    16981 aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg gggcggtaac
    17041 cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc cactaccggg
    17101 aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga tcttccggca
    17161 cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat ttacgctccg
    17221 gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg gcgaactggt ggttccggcc
    17281 ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt acgttactcc gacccagccg
    17341 ttctctgaac tgagcttccg tccgaagaaa gatttgagcg gtgcggatat gtggggagcc
    17401 accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt
    17461 ttcacgccgc catctgaaca gggcacgctg gtcttcccgg gtaacctggg aatgtttgaa
    17521 tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc aatggcactg
    17581 ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc cgatggagca gccgaaagat
    17641 gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc gtatggtgtc
    17701 acgctcaacc cgttcctctc accgtttggt ctgccgtgta aacagccagc atggggttat
    17761 atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg
    17821 caagacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg tatgccgatg
    17881 ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgctgctac ggcagataac
    17941 tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggccg tttaccagcg
    18001 ggtggtcagg cgacgccaat gacctatgaa gtgaatggca agcagtatgt ggtgatctcc
    18061 gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc ttatgcgctg
    18121 ccggatgatg tgaaataacc ataatgcccg gagagatccg ggcatatttc cgaatacggt
    18181 ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt
    18241 gcgccatata agcatgttct ttcggtgcgt tgtgattaaa cttcaccagc acttcactga
    18301 cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc
    18361 cattttctga caagatacgg aactgggtgc cgtgaatatg gaacggatgc agcatcatgt
    18421 cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct
    18481 tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc
    18541 catggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg ctatgatcca
    18601 tcccggccat cgcctgatcg ccatatttct ccatcagcat ctgcatcccc atcatatcga
    18661 gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg
    18721 caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa
    18781 tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg
    18841 gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct
    18901 cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga ccaccgtcgc
    18961 tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac
    19021 agccattaag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt gggtagattg
    19081 caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt
    19141 gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc ggaacatcat
    19201 cgatgcccca ctgttttggc agcattaatt tcaggatctc gtcatcttca attaccacca
    19261 gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt ggatggaacc
    19321 agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcagaa
    19381 taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg
    19441 tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt
    19501 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
    19561 aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
    19621 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc cacagcggca
    19681 aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta
    19741 tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac
    19801 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
    19861 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
    19921 agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga
    19981 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
    20041 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
    20101 gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg
    20161 cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg cccgcgattc
    20221 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
    20281 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
    20341 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
    20401 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat
    20461 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
    20521 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
    20581 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
    20641 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt
    20701 ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc gctttaagct
    20761 ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
    20821 ctccgactgc accgatccta tcggtcccgg cgaaagtctg ttcacctcag cattttatga
    20881 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
    20941 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
    21001 tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc
    21061 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
    21121 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
    21181 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
    21241 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
    21301 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
    21361 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
    21421 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
    21481 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
    21541 agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag
    21601 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
    21661 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
    21721 cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga
    21781 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
    21841 ggtggatgtg aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
    21901 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
    21961 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
    22021 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
    22081 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
    22141 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
    22201 ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg tgtattcatg ccctgcccgt
    22261 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
    22321 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
    22381 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
    22441 tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat cgcagcctta
    22501 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
    22561 caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattacac cgcagtctgg
    22621 aaaatcaccc catcggcttt ttcggtgtac tgagaaagct ggttgaagtt cagataacga
    22681 taagtatcaa cggcagtttt atctacttgc gcaacgtagg tctggtactc ttccggcgtc
    22741 ggcagtttgc caatcagcgc cgcaacagcc gccaattctg cagaagccag gaagacattc
    22801 gcgccagtcc ccagacggtt cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg
    22861 tctgctacac gcgcctggtt acccatacac agggaacagc cagggatctc gatacgcgca
    22921 ccgctcttac cgaagacgct atagtagcct tcttcggtca actgcgcggc atccatacgg
    22981 gttggcggtg ccacccacag gcgcgtcggc aactggcctt tgtgcgcatc cagcagttta
    23041 cccgcagcac ggaagtgacc gatgttggtc atgcaggaac cgataaacac ttcgtcgatc
    23101 ttctcgccct gtaccgcaga cagcggacgt gcgtcgtccg ggtcgttcgg tgcacagagg
    23161 attggctctt taatatccgc cagatcgatg tcgatcactg ccgcgtattc cgcatctgca
    23221 tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat acgacgttcc
    23281 agggtacgac gatcgccgta accttccgcg atcatccact tcagcaggac gatgttagag
    23341 ttcaggtatt cgatgatcgg ttctttgttc agcttgatgg tacaaccagc ggcagaacgc
    23401 tcggcggacg catcggtcag ttcaaatgcc tgctcaactt tcagatccgg cagaccttca
    23461 atttccagaa tgcgaccaga gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga
    23521 ccttgtttga tcgcatacag cgggatcgcg tgtaccagat cgcgcagggt gatgcccggc
    23581 tgcattttgc ctttgaagcg caccagaacg gattccggca tatccagcgg cattacgcct
    23641 gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc gatcgggaaa
    23701 cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg gttcagccag
    23761 gagtggatta cgccgtcacc cggacgcagc gacacaccac cacggttcat aatgaagtcc
    23821 ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt atggcagaaa
    23881 gactgcatca ccaggtcagc cgagaagcct aggcacgcca ggtctttcag ttcatcacgg
    23941 gtcatcgggc cggtggtgtc ctgtgaacct acagaagtca ttttcggttc gcagtacgcg
    24001 cccggacgaa tacctttcac accgcaggca cgacctacca ttttctgcgc cagcgagaag
    24061 ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactgtg cggcagacca
    24121 agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcgggat acggccgcca
    24181 gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgccg
    24241 gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caatcacgtc gcccatgttc
    24301 aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt gttaaagaag
    24361 atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga
    24421 atatcatcgc ccataaacca cagaacggag ttcgtggcgg atttacgcga tgaacccgta
    24481 cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc ttcaatctgt
    24541 ttgatcgggc caacaacacc tggctggtct ggctcaatac cttcacgggc gtttttcagc
    24601 atcgccagcg cgtgcagtgg gatatccggg cgcgaccacg catccggtgc cggagaaagg
    24661 tcatcggtgt tagtttcgcc agtgactttg aagacagtaa cggtcagttt ttcagccagc
    24721 gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcataacctg cttcgcatat
    24781 tcgttgcctg ctttcgcttt ctcttctacg tcatagaagt tatcgaacat cagcagcgtg
    24841 tgagaaagtg ctttggcagc aataggtgcc agtttggcat catccagcgc gtcgatcagc
    24901 gaatgaatgt tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc
    24961 agcagagggg atttggcttc gcctttcgcg atagcagcca ggaagcctgc tttgacatag
    25021 gcggcttcat cgacgcctgg gggaacacgg ttggttaaca gatctaacag gaattcttct
    25081 tcgcccgcgg gcgggttttt cagcagctct acaagtgcgg ccatttggtt tgcatccagg
    25141 ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc
    25201 acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg
    25261 tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt
    25321 gacgatgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga
    25381 tggtctagag attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct
    25441 ttctcaacga atcccaagat tagtcaaaat ttaaactacc gcctctttat actcggattc
    25501 acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga
    25561 aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg
    25621 ccgcctctac cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat
    25681 cgacacaaca gactgcgcct gacatcaccg cagttacgct tcctgcaacg gtttccgcac
    25741 cgcccgtaac gcccgccgtc gtcaaatccg cattcagcac tgcacaaatc gatcaatggg
    25801 ttgcgcctgt cgcgctgtac cccgattctc tgctttcaca agtgttaatg gcatcaacct
    25861 atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg
    25921 atgctgctat tcaggcggta tctgaccagc cgtgggacgc cagcgttaaa tcactggtgg
    25981 cctttccaca attgatggca ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg
    26041 atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg cggcaactgg
    26101 cgcaacaaac cggttcgctg aagtcatcaa ccgaacaaaa agttattacc acaacgaaga
    26161 aagctgtacc ggtaaaacag acagtcacgg cacccgtcat accatccaat accgttttaa
    26221 ctgccagccc cgtcattaca gagcctgcaa caaccgtcat ttccattgag cccgccaatc
    26281 ctgatgtggt ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata
    26341 ctgcgtatcc gccggtttat ctgccaccac cagccggaga accgtttatt gacagctttg
    26401 tgcgcggatt cggctatagc atgggtgttg ctaccacgta cgcactattc agcagcatcg
    26461 actgggatga cgacgatcat gaccatcatc atcatgacga tgatgattat catcaccacg
    26521 atggcggtca tcgtgacggt aatggctggc aacacaacgg cgacaacatc aatatcgacg
    26581 tcaacaattt caaccgtatc accggtgagc atcttactga taagaatatg gcatggcggc
    26641 acaatccaaa ctaccgtaat ggtgtgccct atcatgatca ggatatggca aagcggtttc
    26701 atcaaaccga tgtcaacggc ggaatgagcg ccacgcagct acctgctcca acacgcgaca
    26761 gccagcgtca ggcggcagca agtcagtttc agcaacgaac acacgccgcc cccgtcatta
    26821 cacgagatac ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa aactatggga
    26881 gctatgacga cttccgcgac ttcagccgtc gccaaccact gacccagcaa caaaaggacg
    26941 ccgctcgtca gcgttatcag tcggcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa
    27001 tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt attcagtcag
    27061 cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    27121 agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa cgtcaggttt
    27181 ttcgggaaaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    27241 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
    27301 gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    27361 ccggatgttc cggcaaacga acaattactt cttcttcgct ttcgggttcg gcaggtcggt
    27421 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    27481 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    27541 acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac ggtgagattc
    27601 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
    27661 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    27721 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    27781 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    27841 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    27901 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tccacttcca cgccagcttt
    27961 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    28021 acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    28081 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    28141 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    28201 cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc
    28261 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    28321 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    28381 aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt
    28441 gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    28501 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    28561 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    28621 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    28681 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    28741 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    28801 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    28861 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    28921 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    28981 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    29041 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    29101 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    29161 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    29221 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    29281 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    29341 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    29401 ttcgcccgca gtcaacttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    29461 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    29521 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    29581 cgacaacgaa ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    29641 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    29701 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgagtaa catgcgggat
    29761 catcacccag ttacggctca ggttcgcacc agaaattttc tggatacggc ccagttccac
    29821 ttcttcgatt tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcctgggat
    29881 accgccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    29941 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    30001 aacgccaaac tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt
    30061 ttctgcaaat tcagatttgc cttccgcttt cgcagccggt gctgctgccg gagcttcagc
    30121 ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagccgccg caggcgctgc
    30181 gccttcaact tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac
    30241 tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag ctttgtcgcc
    30301 ttctacggta atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc
    30361 ggtcacttca acttcgtcac cgccgatatc cggaacgtta acttctttca cgccagccgc
    30421 tggtgcaggg gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc
    30481 ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt
    30541 cactttgatc tctttcacgg tgccagcaaa cggagccgga acttccatag acgctttatc
    30601 gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat
    30661 ttcggtcact tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc
    30721 agccgctggt gctgctgccg gagctgcttc tttcttctct tctgcctgag caggtgcagc
    30781 gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc
    30841 gccaacagag actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga
    30901 ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt
    30961 gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat
    31021 agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg
    31081 aatttggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg
    31141 cccagcgccg ctacaaccac gtaggaagca tcaacttcga agtggtgacg caggttctca
    31201 cggctgtcgg aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg
    31261 taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg
    31321 tcgttcatca cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc
    31381 cagcgttcac aatcctgacc atctcgcgcc agttcggtga aggaggtcac gctataaacg
    31441 tcagaaccta cgccgtaatc tttcgccagg atctctgccg cttcacggac gtgacgcagg
    31501 atagaaccgg agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg
    31561 tagatacctt tacggatacc ttcctcagca ccttccggca ttgccggcat gtggtagttt
    31621 tcgttcagcg tggtgatgta gtagtaaacg ttctcttgtt tttcaccgta catacgctcc
    31681 agaccgtcat gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag
    31741 ttcggaatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcgccg
    31801 ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca
    31861 gccgcccagc acagatcgcc aatacgctgg aagccgaaca tcgagtaata gatgtagaac
    31921 gggatcatcg gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct
    31981 gcgcccagct cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa
    32041 gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc
    32101 tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga
    32161 cgatctttga tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg
    32221 gtagagatct ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc
    32281 tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc
    32341 tgagcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcagtttt
    32401 tcgatatctg catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc
    32461 atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg
    32521 taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc ctgcgctttc
    32581 ttgaatgcag cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgc
    32641 tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat atttaccgaa gaagtgttca
    32701 cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc
    32761 atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc
    32821 cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg
    32881 atcttgccgt taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaag
    32941 actaggttat ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg
    33001 tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca
    33061 cggtgttcca gatatttcag gaatttagcc tggtaaatag caccaatcgg ccccagaccc
    33121 atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag
    33181 aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct
    33241 tctaggaaag cacgagcgta cacgcccggg gagatgtggc cctggaagta aaccaggtcg
    33301 ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt
    33361 gcggaagact ggaaggacgc catatggccg cccagttcga ggtctttttt cgacgcacgc
    33421 agcaccgtca tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga
    33481 ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgatacct
    33541 gtgcctgcgg ctacgtttac accgcctttg cgggcttcag caagcagttg gtcgatcaga
    33601 tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg
    33661 cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct
    33721 atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat
    33781 aagacaggtt ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct
    33841 ggataatttt taccagaaaa atcactaatt ctttcgttgc tccagacgac gcagagaacg
    33901 ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt tcttcgataa aggccagatg
    33961 gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg
    34021 ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt
    34081 ctggcggaca ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac
    34141 attgtgggcc gcttcagtga cagcaatctg atactggagt acggcgtttg attccgcgtc
    34201 cagatcgcca gactgctgcg ccagctctat ggcgtggtgg agttcacgga tgcgttcctt
    34261 gtcttcatcg gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg
    34321 tgtttcgagc aagtcatact gtgactcagg atggtcggag agcagctcca ccagcggatc
    34381 gctgaagctt tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa
    34441 caagcccttc gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa
    34501 ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag
    34561 gatcaaaaac tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct
    34621 gtaggccatg agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca
    34681 tgtctgtgac gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa
    34741 tcatgaaact gtgcacattt taacaacttg acatatataa cgtttcaaag ttgtaactat
    34801 gcacaaatgt agactttacg taggaaagga gttttttaac gattattaaa ctataaaaat
    34861 ccgaattgaa ccgattcact taccaatttt gtgattttta attcaattaa aacgaattta
    34921 aattcattct acatattgag aggggttgag gctgagcttt acaaacggtt tctttttaag
    34981 caactcatct tcaaccatgc ataaagcggg tgcattcgct gcctcatacc attattcttg
    35041 atctgacgga agtctttttg taacaattca aacttctttg atgtaaacaa attaatacaa
    35101 caaacggaat tgcaaactta cacacgcatc actgcgtaga tcaaaaaaac aaccaccgca
    35161 cgaggtttca tgatggaagg tcaacagcac ggcgagcagc taaagcgcgg ccttaaaaac
    35221 cgccatattc agcttatcgc gctgggtggc gcgataggga ccgggttatt cctgggtagc
    35281 gcctccgtaa tacagtccgc agggccaggg attatcctgg gttacgccat tgctggtttt
    35341 atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg tcgaagaacc tgtcgcaggc
    35401 tcctttagcc actttgctta taaatactgg ggcagttttg ccggtttcgc ctctggctgg
    35461 aactactggg tactgtacgt tttagttgcc atggctgagc tgactgccgt gggtaaatac
    35521 attcagttct ggtatccgga aatccccacc tgggtttctg ccgccgtatt ctttgtggtg
    35581 attaacgcca tcaacctgac caacgttaaa gtgtttggcg agatggagtt ctggtttgcc
    35641 attatcaaag ttatcgcggt ggtagcgatg atcatcttcg gcggctggct gctattcagt
    35701 ggcaacggcg gcccgcaggc gaccgttagc aacctgtggg atcagggtgg tttcctgccg
    35761 cacggcttca ccgggctggt gatgatgatg gcgatgatca tgttctcgtt cggtggtctg
    35821 gaactggtgg ggatcaccgc agcagaagct gataacccgg agcaaagtat accgaaagca
    35881 actaaccagg ttatctaccg catcctgatt ttctatattg gttcgttagc cgttctgctc
    35941 tcactgatgc cgtggacccg cgttaccgcc gataccagtc cgtttgtgct gatcttccac
    36001 gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg tggtactgac tgcggcgctc
    36061 tccgtgtaca acagctgcgt atattgcaac agccgtatgc tgtttggtct ggcacaacag
    36121 ggtaatgcgc caaaagcgct ggcgtctgtc gataaacgtg gtgtaccagt aaataccatt
    36181 ctggtgtctg cactggtaac ggcgttgtgc gtactgatta actaccttgc cccagagtcc
    36241 gctttcggac tgttaatggc gctggtggta tctgcactgg taatcaactg ggcgatgatt
    36301 agcctggcgc atatgaaatt ccgtcgcgcc aagcaggaac aaggcgtggt aactcgcttc
    36361 cctgctctgc tttatccgct gggtaactgg atctgcctgc tgtttatggc ggcggtactg
    36421 gtgattatgc tgatgacccc aggaatggcg atttcggtat acctgatccc ggtatggctg
    36481 atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat
    36541 taatctctct acgccctcac ccgtacaggg tgagggcaat aatctttaca ccaacgcccc
    36601 gtaaatcgtc agtagtgcaa tcaccaccac gaccacgaac gaggttttct tcgccattga
    36661 aaccgctgcc ctcggcgtct ccaccttatc gacatgcggt tcacgcgcca gagagaactg
    36721 cgccagacgc gttaacacct gatactgcga agtatggaaa tcacccagcg aagcaaacca
    36781 ggccggtaac gctttctcac catgaccgat caaggcatat accacacccg caagacgaac
    36841 cggcacccaa tccagtacat gaagcacggc atcaatgccg gactgtaaac gatgatgcgg
    36901 cgtctgatat cgcgccagcc agtattgcca tgcacgcaaa aacgcatacc ccatcagcgt
    36961 aacgggtccc caggttcccc ccacaatcag ccagaacagc ggtgcaagat aaaaacgaaa
    37021 gttaatccac agcaatgcat tttgcagctc acgcaaatac tcacgttcgt cgcagcctgc
    37081 cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg gcacgggcat ggctatcatt
    37141 acgtgaagca gctgtcagat aagcatgata atgaagacga actttacctg cgccaataca
    37201 cagcaaacca atcagcagcc acaccagtag cgtgggaacg ttgaacaata ctccctgcaa
    37261 tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg gtcatgccta acgtgcgccc
    37321 gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga cgatgatcaa gctgccagtg
    37381 ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt aaggttgtaa atagcgtcat
    37441 gttgtctcct tgctgaccag cacacgaaac cgtgcccaat caaatgcagg accgggatcg
    37501 gttttccgat ccggcgcaat atcacaatgg cccgtcatgt ttttagcgat atccggatag
    37561 caatcaatca gtgcccgcgt aaccgccgca agctgttgat actgcgcatc ggtatacgcc
    37621 agcgtatcgg tgccttcaag ctcaatccca atagaaaaat cattgcagcg ttcgcgcccc
    37681 tgatactgag agactcccgc atgccatgca cgtttatcga aaggaacata ctggactatt
    37741 tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca aatgggcgat ctcagcaaag
    37801 aaaggatgtg cctgcggatc aatagttcca gtgaataatg cgtcgatcca cggaccgcca
    37861 aactcgcctg gcggaaggct aatattgtgc accaccagca gggtgggtgt ttcgtcatcc
    37921 gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc caaccagcca cccctgttct
    37981 aacaacatgc aggatctcct tatatgtggt gctaataccc ggttcagagt agcatgtttc
    38041 taccttatga ttcgttagct atctggagtt ttaacatgcc gcctcgccgc tataaccctg
    38101 acacccgacg tgacgagctg ctggaacgca ttaatctcga tatccccggc gcggtagccc
    38161 aggcgctgcg ggaagattta ggcggaacag tcgatgccaa caatgatatt acggcaaaac
    38221 ttttaccgga aaattctcgc tctcatgcca cggtgatcac ccgcgagaat ggcgtctttt
    38281 gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc aggcgacgat gtcaccataa
    38341 tctggcatgt ggatgacggc gatgtcatca atgccaatca atccttgttc gaacttgaag
    38401 gcccatcccg cgtgctgtta acgggcgaac gcactgcgct taattttgtg caaacccttt
    38461 caggagttgc cagtaaggta cgccactatg tcgaattgct ggaaggcacc aacacgcagt
    38521 tgttggatac gcgcaaaacc ttacccggcc tgcgttcagc tctgaaatac gcggtacttt
    38581 gcggcggcgg agcgaatcac cgtctggggc tttctgatgc cttcctgatc aaagaaaacc
    38641 atattattgc ctccggctca gtgcgccagg cggtcgaaaa agcgtcctgg ctgcacccgg
    38701 atgcgccagt agaagtcgaa gtagagaatc tggaagaact tgatgaagcc ctgaaagcag
    38761 gagccgatat catcatgctg gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac
    38821 gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt cactgacaaa acactgcgtg
    38881 aatttgccga aacgggcgtg gactttatct ccgtcggtgc gctaactaaa cacgtacaag
    38941 cactcgacct ttcaatgcgt tttcgctaat agttgacaga tttatccgct ccatcgcggg
    39001 cggataatct ccccttcccc aactttcttc gtaacgcctc gcaaatttct tgtctttcgc
    39061 tgcaacgaaa taaaaaacct cggaatctgc tttcccgttt cgttttttgc ccctcgcctg
    39121 tgctgatggc gtttgccaaa gtagcgccaa ctaaatcaag gagcgaaaca gatggacaag
    39181 caacgcggtt ttacacttat cgaactgatg gtggttattg gcatcattgc cattttaagc
    39241 gccattggta ttcccgctta tcaaaactac ctgcgcaaag ccgcactcac cgacatgcta
    39301 caaacctttg tgccttaccg taccgccgta gagttgtgcg cgctggaaca tggtggatta
    39361 gatacctgcg acggtggcag caatggcatt ccctcgccta ccaccacccg ctatgtttca
    39421 gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc aagaaagtct caatgggcta
    39481 agcgtcgtca tgacaccggg ttgggataac gcaaacggcg tcaccggctg gacgcgcaac
    39541 tgcaatattc aaagtgacag cgcattgcat caagcctgcg aagatgtctt ccgctttgat
    39601 gacgccaact aaggagcggc aatgaatatt ccacagctca ctgccctgtg tctgcgttat
    39661 catggagtct tgctggatgc cagcgaagag gtggttcatg ttgcggtagt cgatgcacct
    39721 tcgcatgagc tactggacgc attgcatttc gctaccacca aacgtattga gatcacctgc
    39781 tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac aacagacatt gcccgtagct
    39841 gttcaggaga agcatcagcc caaagcagag ttgctaactc gaacgttaca atctgcgctg
    39901 gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca atgcctaccg catccgcttg
    39961 cgtatcgacg gcgtattgca tcctttaccg gatgtttcac cggatgccgg agtcgcatta
    40021 accgccagat taaaagtgct gggaaacctg gatattgcgg aacatcgcct gccgcaggac
    40081 gggcaattca ctgtcgaact ggcaggaaac gccgtctcat ttcgtattgc gaccttacca
    40141 tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc aggtgagcca ggcactggat
    40201 gtcaacacgc ttggaatgca gccgttacaa ctggcggact ttgctcatgc cttgcaacaa
    40261 ccacagggac tggtgctggt aactggccct acaggcagcg gcaaaacggt cacgctttat
    40321 agtgccctgc aaacgctgaa taccgctgac attaatattt gtagcgtcga agatccggtt
    40381 gagatcccca tagccggact aaaccagacg caaatccatc cgcgtgccgg actcaccttt
    40441 cagggcgtgt tgcgtgcgtt attgcgccag gatcctgacg tcatcatgat cggagagatc
    40501 cgcgatggcg aaacagcaga gatcgctatt aaagcggcgc aaactggtca cctggtgttg
    40561 tctaccctac acactaattc cacctgcgaa acgctggtac gtttacagca aatgggggtc
    40621 gcccgctgga tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgcaaa
    40681 ctttgcccac attgtcgccg gcagcaaggg gaacccatcc acattccaga caatgtatgg
    40741 ccatcgccgc tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat
    40801 ggtcgtacgg ccttatttga agttctgccc ataacgccgg tcattcgtca gcttatttcc
    40861 gctaataccg acgttgaatc gctggaaacg cacgcacgac aggcgggtat gcgtacgctt
    40921 tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc
    40981 gtactgggga tgccgcatgg cgagtaagca actctggcgc tggcatggca taaccggcga
    41041 cggcaatgcg caagatggga tgctatgggc agagagccgt gctttgctgc tcatggcact
    41101 acagcaacag atggttaccc cacttagcct gaagcgaatc gccatcaatt ctgcgcagtg
    41161 gcgaggagat aaaagcgcgg aagtcattca tcaactggcg acgctactca aagccgggtt
    41221 aacgctttct gaagggctgg cactgctggc ggaacagcat cccagtaagc aatggcaagc
    41281 gttgctgcaa tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt
    41341 accctggtca gaggtatttc cgccactcta tcaggcgatg atccgcacgg gtgaactgac
    41401 cggtaagctg gatgaatgct gctttgaact ggcgcgtcag caaaaagccc agcgtcagtt
    41461 gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc attttagcga tggcaatcat
    41521 ggtggttgtg gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt
    41581 caacacccca ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga
    41641 atggagctgg ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat
    41701 gcgccgaccg acctggctta tagtgcggca gaaattgctg ttacgcatcc cgattatggg
    41761 ttcactgatg cggggacaaa aactcacgca gatttttacg attctggcgc tgacacaaag
    41821 tgcaggcatt acttttttgc agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta
    41881 ctgggtgcaa cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct
    41941 ggcgctaaaa aataccgggg agtttagccc gctctgtttg caattagtga gaacaggaga
    42001 ggcatccggc tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac
    42061 aatggcgctg gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg
    42121 aggaattatc ggtacgctgg tggtggcgat gtatctgcca attttccatt taggcgatgc
    42181 gatgagtggg atgggataac gctggcgtgt tgctccacac cagcgttgga agattacagg
    42241 ttgttgaaga tgcggttttc ttgttcctgc acacggataa atgtggtgcg cttggtcagt
    42301 tctttcaggc gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca
    42361 cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg
    42421 cgatattccg caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag
    42481 aacagcataa atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc
    42541 gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt tgcgacatcg
    42601 cccggcgtgg tgcagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg
    42661 gcacattcga ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta
    42721 caaacagaac ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc
    42781 tcacacattt caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca
    42841 cgcgctttcg caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa
    42901 acgaagttta atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca
    42961 gacgtaccgg tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac
    43021 gcttgccact cttcgacaga atagtgttta tgcacagcag tcaaaatatc aaaagaagcc
    43081 agcgcagagg ccatagaaaa tgtacctacg gtgtccatat ttgcggcgat aattggcacg
    43141 ccggaccagc tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa
    43201 cggcttttaa gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga
    43261 tcttcttcaa tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa
    43321 ctccagtgac gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt
    43381 tttacgctac aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc
    43441 gctttcgtgc actcattttt aacgttttga taatcatgat gaattcccgg gaatataaat
    43501 tatgaggtat atagttgcct taacgggagg cattggcagt ggcaagagta ccgttgccaa
    43561 tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt
    43621 tgaaccaggt gcacctgcgc tacatgccat tgctgatcac tttggcgcta acatgattgc
    43681 tgctgatgga acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga
    43741 gaaaaactgg cttaacgccc tgctgcatcc gctgattcag caagagacgc aacaccagat
    43801 ccagcaagct acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact
    43861 gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg
    43921 caccatgcag cgcgatgatg taactcgcga gcatgtcgaa caaatccttg ctgctcaggc
    43981 aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga
    44041 tgctatcgca tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt
    44101 tgtctcacag gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa
    44161 aaaatgcgta catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc
    44221 atcgttgacc acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt
    44281 ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac ttgaccggca gcaacgtaaa
    44341 ctccagacct ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag
    44401 cagttaaaag cggcggggag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt
    44461 gaagatcgtt tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc
    44521 tttgatttac ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag
    44581 gtagaaacct ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat
    44641 ttaattcgcc agtcggctcc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat
    44701 aacggtggcg atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg
    44761 caaatttccg gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac
    44821 ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt gaaaatgtca
    44881 gaaactatta cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc
    44941 ccgtttcggc cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct
    45001 gaagaaaaac gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag
    45061 ccaaagcagt gacatttgtc tgatgccgca cgtagatact gttatggcct gcaagttttt
    45121 tcctggtccg gtttcccgga ttgcaggcat gccagacaca gcgccg
//
LOCUS       DAZFDP010000021        45109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFDP010000021 DAZFDP010000000
VERSION     DAZFDP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..937)
                     /locus_tag="ACHJ1G_001275"
     CDS             complement(<1..937)
                     /locus_tag="ACHJ1G_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4763164.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQESDRQKGKKSWQAERYARGNLRKIFERLERTDHRWL
                     TPGYRSLTGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDSFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFR"
     gene            complement(934..1323)
                     /locus_tag="ACHJ1G_001276"
     CDS             complement(934..1323)
                     /locus_tag="ACHJ1G_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO4763165.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPILRGLFDLYIRGRDNRARTTKPEN
                     VDTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCVS
                     LSEYTAEFNRLSTIFLQEWQRVTGYQP"
     gene            complement(1647..1850)
                     /locus_tag="ACHJ1G_001277"
     CDS             complement(1647..1850)
                     /locus_tag="ACHJ1G_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763166.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            complement(1938..2051)
                     /locus_tag="ACHJ1G_001278"
     CDS             complement(1938..2051)
                     /locus_tag="ACHJ1G_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763167.1"
                     /translation="MSIFISWLVLIISVVCAIGIMQIIHSVKKIERIFTGE"
     gene            complement(2048..2290)
                     /locus_tag="ACHJ1G_001279"
     CDS             complement(2048..2290)
                     /locus_tag="ACHJ1G_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4763168.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(2302..2580)
                     /locus_tag="ACHJ1G_001280"
     CDS             complement(2302..2580)
                     /locus_tag="ACHJ1G_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763169.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            complement(2591..2929)
                     /locus_tag="ACHJ1G_001281"
     CDS             complement(2591..2929)
                     /locus_tag="ACHJ1G_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4763170.1"
                     /translation="MLKQRRNFRTETERQANRFATSASRSNIRYSLSETHATPDGHTV
                     KQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDTQFGQDFTLYEALRTVDRLLR
                     GQSFIKQTDL"
     gene            complement(2944..3222)
                     /locus_tag="ACHJ1G_001282"
     CDS             complement(2944..3222)
                     /locus_tag="ACHJ1G_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4763171.1"
                     /translation="MTKLLNTYEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     TVNAVKRQADRAALPINQEEKNSKRTVNLFAIFLKTIRNAEKYVQMTK"
     gene            3314..3625
                     /locus_tag="ACHJ1G_001283"
     CDS             3314..3625
                     /locus_tag="ACHJ1G_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4763172.1"
                     /translation="MSSGYEKKLKEIRKSEGLTQAEFADVTGINLGTIKNYESGKREV
                     GLSVVDRVINSKDFEKYTIWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            3714..4649
                     /locus_tag="ACHJ1G_001284"
     CDS             3714..4649
                     /locus_tag="ACHJ1G_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4763173.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYPLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPIAG"
     gene            4660..5055
                     /locus_tag="ACHJ1G_001285"
     CDS             4660..5055
                     /locus_tag="ACHJ1G_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763174.1"
                     /translation="MEKTVNSDLHLGQYWRIDIVCDPELRDEVEQYFSLHDVGFSKIE
                     VFSVENPYKLALFFDFAKKGVEVAKAIMGLLDRNDIEITMYRATDSSPQSVKSIKLRK
                     SEDVEKCKDLLSTCVAIGVQRNKEKEGSE"
     gene            5245..6225
                     /gene="btuC"
                     /locus_tag="ACHJ1G_001286"
     CDS             5245..6225
                     /gene="btuC"
                     /locus_tag="ACHJ1G_001286"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4763175.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            6288..6839
                     /gene="btuE"
                     /locus_tag="ACHJ1G_001287"
     CDS             6288..6839
                     /gene="btuE"
                     /locus_tag="ACHJ1G_001287"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO4763176.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            6839..7588
                     /gene="btuD"
                     /locus_tag="ACHJ1G_001288"
     CDS             6839..7588
                     /gene="btuD"
                     /locus_tag="ACHJ1G_001288"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4763177.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            7666..8130
                     /gene="nlpC"
                     /locus_tag="ACHJ1G_001289"
     CDS             7666..8130
                     /gene="nlpC"
                     /locus_tag="ACHJ1G_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4763178.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            8377..9090
                     /gene="ydiV"
                     /locus_tag="ACHJ1G_001290"
     CDS             8377..9090
                     /gene="ydiV"
                     /locus_tag="ACHJ1G_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4763179.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            9153..10589
                     /gene="selO"
                     /locus_tag="ACHJ1G_001291"
     CDS             9153..10589
                     /gene="selO"
                     /locus_tag="ACHJ1G_001291"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4763180.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(10593..10784)
                     /gene="hemP"
                     /locus_tag="ACHJ1G_001292"
     CDS             complement(10593..10784)
                     /gene="hemP"
                     /locus_tag="ACHJ1G_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4763181.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(10916..11962)
                     /gene="aroH"
                     /locus_tag="ACHJ1G_001293"
     CDS             complement(10916..11962)
                     /gene="aroH"
                     /locus_tag="ACHJ1G_001293"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4763182.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(12119..12952)
                     /gene="ppsR"
                     /locus_tag="ACHJ1G_001294"
     CDS             complement(12119..12952)
                     /gene="ppsR"
                     /locus_tag="ACHJ1G_001294"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4763183.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            13285..15663
                     /gene="ppsA"
                     /locus_tag="ACHJ1G_001295"
     CDS             13285..15663
                     /gene="ppsA"
                     /locus_tag="ACHJ1G_001295"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4763184.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(15720..17366)
                     /gene="fadK"
                     /locus_tag="ACHJ1G_001296"
     CDS             complement(15720..17366)
                     /gene="fadK"
                     /locus_tag="ACHJ1G_001296"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO4763185.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(17423..17716)
                     /gene="ydiT"
                     /locus_tag="ACHJ1G_001297"
     CDS             complement(17423..17716)
                     /gene="ydiT"
                     /locus_tag="ACHJ1G_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4763186.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(17713..19002)
                     /gene="ydiS"
                     /locus_tag="ACHJ1G_001298"
     CDS             complement(17713..19002)
                     /gene="ydiS"
                     /locus_tag="ACHJ1G_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4763187.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(19058..19996)
                     /gene="ydiR"
                     /locus_tag="ACHJ1G_001299"
     CDS             complement(19058..19996)
                     /gene="ydiR"
                     /locus_tag="ACHJ1G_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4763188.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(20016..20780)
                     /gene="ydiQ"
                     /locus_tag="ACHJ1G_001300"
     CDS             complement(20016..20780)
                     /gene="ydiQ"
                     /locus_tag="ACHJ1G_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4763189.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLTKALN"
     gene            21096..22007
                     /gene="ydiP"
                     /locus_tag="ACHJ1G_001301"
     CDS             21096..22007
                     /gene="ydiP"
                     /locus_tag="ACHJ1G_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4763190.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(22050..23201)
                     /gene="ydiO"
                     /locus_tag="ACHJ1G_001302"
     CDS             complement(22050..23201)
                     /gene="ydiO"
                     /locus_tag="ACHJ1G_001302"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4763191.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITSAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(23215..24809)
                     /gene="ydiF"
                     /locus_tag="ACHJ1G_001303"
                     /pseudo
     CDS             complement(23215..24809)
                     /gene="ydiF"
                     /locus_tag="ACHJ1G_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(24953..25711)
                     /gene="aroD"
                     /locus_tag="ACHJ1G_001304"
     CDS             complement(24953..25711)
                     /gene="aroD"
                     /locus_tag="ACHJ1G_001304"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4763192.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(25742..26608)
                     /gene="ydiB"
                     /locus_tag="ACHJ1G_001305"
     CDS             complement(25742..26608)
                     /gene="ydiB"
                     /locus_tag="ACHJ1G_001305"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4763193.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(26620..27885)
                     /gene="ydiN"
                     /locus_tag="ACHJ1G_001306"
     CDS             complement(26620..27885)
                     /gene="ydiN"
                     /locus_tag="ACHJ1G_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763194.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(28112..29326)
                     /gene="ydiM"
                     /locus_tag="ACHJ1G_001307"
     CDS             complement(28112..29326)
                     /gene="ydiM"
                     /locus_tag="ACHJ1G_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763195.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(29426..29782)
                     /gene="ydiL"
                     /locus_tag="ACHJ1G_001308"
     CDS             complement(29426..29782)
                     /gene="ydiL"
                     /locus_tag="ACHJ1G_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4763196.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(29918..30025)
                     /gene="rprA"
                     /locus_tag="ACHJ1G_001309"
     ncRNA           complement(29918..30025)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ1G_001309"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(30211..31323)
                     /gene="ydiK"
                     /locus_tag="ACHJ1G_001310"
     CDS             complement(30211..31323)
                     /gene="ydiK"
                     /locus_tag="ACHJ1G_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4763197.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            31712..34768
                     /gene="ydiJ"
                     /locus_tag="ACHJ1G_001311"
     CDS             31712..34768
                     /gene="ydiJ"
                     /locus_tag="ACHJ1G_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4763198.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            34765..35175
                     /gene="menI"
                     /locus_tag="ACHJ1G_001312"
     CDS             34765..35175
                     /gene="menI"
                     /locus_tag="ACHJ1G_001312"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4763199.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            35275..35463
                     /gene="ydiH"
                     /locus_tag="ACHJ1G_001313"
     CDS             35275..35463
                     /gene="ydiH"
                     /locus_tag="ACHJ1G_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4763200.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            36011..36379
                     /gene="sufA"
                     /locus_tag="ACHJ1G_001314"
     CDS             36011..36379
                     /gene="sufA"
                     /locus_tag="ACHJ1G_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4763201.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            36388..37875
                     /gene="sufB"
                     /locus_tag="ACHJ1G_001315"
     CDS             36388..37875
                     /gene="sufB"
                     /locus_tag="ACHJ1G_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4763202.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            37885..38631
                     /gene="sufC"
                     /locus_tag="ACHJ1G_001316"
     CDS             37885..38631
                     /gene="sufC"
                     /locus_tag="ACHJ1G_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4763203.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            38606..39877
                     /gene="sufD"
                     /locus_tag="ACHJ1G_001317"
     CDS             38606..39877
                     /gene="sufD"
                     /locus_tag="ACHJ1G_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4763204.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            39874..41094
                     /gene="sufS"
                     /locus_tag="ACHJ1G_001318"
     CDS             39874..41094
                     /gene="sufS"
                     /locus_tag="ACHJ1G_001318"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4763205.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            41107..41523
                     /gene="sufE"
                     /locus_tag="ACHJ1G_001319"
     CDS             41107..41523
                     /gene="sufE"
                     /locus_tag="ACHJ1G_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4763206.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            41672..42676
                     /gene="ldtE"
                     /locus_tag="ACHJ1G_001320"
     CDS             41672..42676
                     /gene="ldtE"
                     /locus_tag="ACHJ1G_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4763207.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(42740..42976)
                     /gene="lpp"
                     /locus_tag="ACHJ1G_001321"
     CDS             complement(42740..42976)
                     /gene="lpp"
                     /locus_tag="ACHJ1G_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4763208.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43287..44699)
                     /gene="pykF"
                     /locus_tag="ACHJ1G_001322"
     CDS             complement(43287..44699)
                     /gene="pykF"
                     /locus_tag="ACHJ1G_001322"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4763209.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(44707..44901)
                     /locus_tag="ACHJ1G_001323"
     CDS             complement(44707..44901)
                     /locus_tag="ACHJ1G_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763210.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT        11303 a        10892 c        11548 g        11366 t
ORIGIN      
        1 cgcgaaactc gcttaatgcg tcctgactca gatagggcga tgtttttctg gaaaccagac
       61 aggcggcgcg gagttgttct tcccgccact cgcaacgtaa cagccacagt ttgcgtttcc
      121 accattccgc acaggtcagg cgaaggattg cgcccggcag cagctccgtg tccggttcgt
      181 tcctccggtc tttgtctgtt gtcagtgcgt cataatgtgg aggcatggcg tgcaggtgta
      241 acgccatgcg ggccagcatc tgatacgcct tcagcgttac atccatggtc agctcgccat
      301 cagtcgcgcc aaagctatcg cagagttttt cgaaggtgct gctgaacatc gccgccgtca
      361 tggtggccag cgtctgtatc tggtgtttgt tgagctgcgg caggtaaagc aaatcgtcca
      421 ggcgttcgcg tccggtaagg gagcgataac ccggtgtcag ccagcggtga tcggtgcgtt
      481 ccaggcgttc gaatattttg cgcaggtttc cgcgcgcgta gcgttcagcc tgccagctct
      541 ttttgccttt ctgacgatcg gattcctgtt ttttgcgcag gaaagagagg tggcggctca
      601 gaggttcacg cagataaacg ggaagcactt tcagtgtggc aaaagcacgg gccaccgggt
      661 cttgttctgt tgcctgacgc ttgctgatga tgctttgtgc cagcttttca cgctgtccgg
      721 cttcctcaag ggatgccatg agttttttac ccacggtgga ttgtgcgaaa aaggcttcct
      781 ccttcgcttc ctgttcttcc agggcctttt tgtctgcctc aaggtagtaa cggatggcgc
      841 gttgcaggtc ggtttcagtt tcctgcctgt gctccgtaaa tctggccgga tcaatggctg
      901 gtcgtggttc attccagctc catgcaaact cactcatggc tggtatcccg tcacgcgctg
      961 ccactcctgc aagaagatgg tggaaaggcg gttaaattca gcggtgtatt cactcagcga
     1021 gacacacccg ccagcagtgc gatgcgccag cattgccgca aatacggagg ccggggagtc
     1081 gtaatacgcc agcagtgatt cgccgtgcgg tgtcaggcag tgcaacgcca gtccgtgtgg
     1141 tgtgaagtcc acgcggtagc agtcgtctac tgtgaaataa agggtatcca cattctccgg
     1201 ttttgtggtg cgtgctctgt tgtcacgacc acgaatgtag agatcaaata atccccgaag
     1261 aatgggagcc agacgggtgt cctgtgtgcg cacccatctt gtgaagtcat gagcgtcaat
     1321 catgctgcaa ttctctctac taaggatgtg cgaaggcttc ctgccaaaaa actcagaagc
     1381 ggtcggttac agaaattggc gttctacgag tagttattgc tggcttgagg ttcatttctg
     1441 tttaattccg tgcatgctgt agaagaaatc aaaagtgtta tctatgcgta tcatgagctc
     1501 acgctgcatc gcttctggtg tttctggttc acctagtgag ccaactcccg cgaagaaatc
     1561 accgatctcg ctttgaataa gtgcctttag cgttgctgag gaattcaggt gtgtgcggtg
     1621 atgtctgcga gtgattcttc tcctgctcat ttgcgaggat cctgtacctg tcggataaga
     1681 ttcacccgcg ccacattagt ggcgcagaag taagtaccgt cagtgagata gatgtggtgt
     1741 gcatcctttt ctgagcggtg tttgtcgatt gtggtaatca ggcgttcgtc gacttcgtat
     1801 tcacgtcctc tggaagtgaa acgaacgaca ggaaaatgct taattgccat tacgcctcct
     1861 gggcgtgtgc gaatacctcc gcgaatgcgg attgttttta cattttctta tttaacctag
     1921 ggtttttatt tgcgctgtta ttcgccagtg aaaatgcgtt caatcttttt tactgaatga
     1981 ataatttgca taatcccaat ggcgcagacc accgaaataa tcaggacaag ccatgagata
     2041 aatatactca tgcaatattc cccagcttat acggttcaat atgctccccg cattctgcgg
     2101 cacagatcag ctcggaaagt tcgttaagtg catccagatc atcagcgtaa aaagccacgt
     2161 catacagact ccggattgcc ctggtcaatg agtcacgggc tgcacgttca gcatgagcac
     2221 ctgatgcact taagcgaaaa taaaaacgct caagtgcttt gttaatgaga gttttatatt
     2281 ctttgcccat cgcaacgccc tttaatctgc tttctgaatt tcagcttctg aatccataca
     2341 aataatttcg atatagggtt catcgccatt aatcttacgt gctttttcag cttcgctaat
     2401 gattccgtgt acagtctggt acggaagttc tacggtcagg cgcgtgccgt tcagataaac
     2461 gtaagtagct gcattttttt ctgatgggac aactccatca atagctgatg cgcgcaataa
     2521 cagttcaccg cgaaaatcaa taaaacggat aaatacacct tgtgcatgct ctttggtcat
     2581 aacgcacctg ttataaatca gtctgtttaa taaaactttg cccgcgaagc agacgatcaa
     2641 ccgtgcgaag tgcttcgtac aatgtgaaat cctgtccaaa ctgagtgtcg ccgttgctca
     2701 gagcaaaaat gcggtttccg gtaaatgggt tgcgttggca tctgtgaacc acgattccag
     2761 ctttttcaat cagccaggtg tgttcgccaa tttgttttac tgtatggcca tctggcgttg
     2821 cgtgcgtctc gctcaggctg tagcggatgt tgctgcgtga tgcgctggta gcgaaacggt
     2881 tagcttggcg ttccgtttcg gtgcgaaaat tacggcgttg cttcagcata aaatgacacc
     2941 tcgttatttt gtcatctgca catatttctc tgcgtttctg atggttttca ggaaaattgc
     3001 gaagagattt actgtacgtt ttgagttttt ttcttcttgg ttgatgggaa gggctgctct
     3061 gtcagcctgc cttttcactg cattaacagt ttgattggta cgcttcgcgt aatctttcag
     3121 gctttcttcg agtaccggta atccatgctc atcgcggtat gggtagaacg ccgccaaacg
     3181 ctcaaaatcc gcttgctcgt atgtgttcaa gagctttgtc atgatgtgat aacctgttca
     3241 gtctgtggtt atttgttgct aaaagtcgtc tctaggcgac ttttagggtt aatttagtcg
     3301 tctggagacc accatgtcaa gtgggtacga aaaaaaactg aaagagatac ggaaaagtga
     3361 agggttaact caagctgagt ttgcagatgt tactgggata aatctcggaa ctataaagaa
     3421 ttatgagagc ggtaaaagag aggttggttt aagcgttgtt gatcgcgtaa ttaattctaa
     3481 ggatttcgaa aaatacacta tatggcttat gacgggaaaa acaaatgagg ctgctgggca
     3541 gatcagtccc tctctctccc ctgatgggcc agaaaacaca tcgtcttctc aaaaatcccg
     3601 caagactggt acacagcccg gctaatcatg gaacgctggg ggcatggtgg tcttgtaacg
     3661 ctggggtttc acgaatgagc ataaaatcaa ttccgggagg gtatcttctt gacatgcgcc
     3721 ctgaggggcg taaaggcaaa cgcattcgca aaaaatttaa aacgaaatcg gatgcagttt
     3781 tatatgagcg gtgggtgctg gcgcaacagc ataacaatga gtggaaagga aactccattg
     3841 atcgccgtcc gctgtcggtg cttattgact tgtggtggaa ataccacggc cagctaatga
     3901 agtcagggca taacacgcgc cttaaattgc tgcgcttgag tgaggcaatg gatgacccgt
     3961 gtgtgcataa acttaataca acgatgctca ccgagctacg tgtgtccagg atagagcagg
     4021 ggatacagcc cagcaccatt aatcgagaga ttggggcgtt aagcgcgatg tttaccgcac
     4081 tcatctcatc cggccatttt cttaacgata accccgttca aggccttaaa ggaatgaagg
     4141 ttaacgagcg cgaaatggga tatctgagta agtctgaatg tgttcagttg ctggatgcac
     4201 tggctgaaaa tcccgatgaa cggctggctg tcgaaatcct tctgtcgacc ggggcgcgat
     4261 ggggcgaggt agcggcactg gagcagcgcc gtgttcttca ttgtcgaatc actttttcaa
     4321 aaacgaagaa cagcaaaaac cgtaccgttc ctatttctga aagcctgttt gaaaagatca
     4381 aaaaacgggg cgggaaactg gtgtttccga cgctggatta tccattggtt cgcgatgtca
     4441 tcaaaacggt cgcacctgat gttcctgacg gccaggctgt tcatgcgctg cgccacacct
     4501 tcgccagtca tttcatgatg aacggcggca atattctgac gctccagaaa attctggggc
     4561 acgcaaagat tcagacaacg atgatttatg cccatcttgc gccggattac ttgcaggatg
     4621 cggtgagatt taatcctatt gctgggtaag gaggatgttg tggaaaaaac agttaatagc
     4681 gatttgcatt taggtcagta ctggagaata gacattgtat gcgatccaga acttcgagat
     4741 gaggttgagc aatatttttc tctacacgat gttggtttta gcaaaattga agttttttcc
     4801 gtcgaaaatc catataaact tgctctgttc tttgatttcg cgaagaaggg tgtggaggtt
     4861 gcaaaagcca ttatgggact cttagaccga aatgatattg aaataacgat gtatcgtgct
     4921 acggacagta gcccgcagtc tgtaaaaagc atcaaattgc gtaagtctga agacgtagag
     4981 aaatgtaaag atttgcttag tacgtgtgtc gcaattggcg ttcaacgaaa taaggaaaaa
     5041 gaggggagcg aatgatccac aaagtgacca catccctgtt atttgttgtg gttggctgtg
     5101 tttttgtgtg tctgtaagtc tttgataatt atctaactta ttgatttttg tttgtgttta
     5161 tggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca
     5221 tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca gcgacaaaat
     5281 attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt aagcctttgc
     5341 gccggtgaac aatggatctc gccaggtgac tggtttactc ctcgtggcga actgttcgtc
     5401 tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc gctggctata
     5461 tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg actacttggc
     5521 gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca agggcaactc
     5581 cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac tttaatactc
     5641 ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg cgttgcatta
     5701 gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc tgttgattta
     5761 cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg gcaaagctgg
     5821 ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag gccgatgaat
     5881 atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg gttctggcgc
     5941 aatgtgctgg tggccgcgac cggctggatg gttggcgtca gtgtggcgct ggcgggtgct
     6001 atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt aaccgatcat
     6061 cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct ggccgatatt
     6121 gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac cgcaacgttg
     6181 ggtgcgccgg tgtttatctg gttattgtta aaagcaagac gttagccgca aaaagacggt
     6241 ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg caagattcca
     6301 ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag aagtacgctg
     6361 gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg caatatgagc
     6421 agttggagaa tattcagaaa gcctgggccg atcgaggttt tgtggtgctg ggattcccgt
     6481 gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact tactgtacca
     6541 ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc gaaggacgcc
     6601 atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg gaagagagcg
     6661 gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat gatattttat
     6721 ggaattttga gaaattttta gttggcaggg acgggttggt catccagcgt ttttccccgg
     6781 atatgacgcc tgaagatccc attgtgatgg aaagcattaa actggcgttg gcaaaataat
     6841 gtctattgtg atgcagttac aggatgttgc ggaaactacc cgcctggggc cgctttctgg
     6901 cgaggttcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg gtaagagtac
     6961 cttgcttgca cgaatggccg gaatgaccag cggtaaggga agcattcagt tcgcggggca
     7021 accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctacc tttcacaaca
     7081 gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc agcacgataa
     7141 aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg acaaactcgg
     7201 acgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt
     7261 gctgcaaatc acaccacaag ccaatcccgc aggtcaattg ctgcttcttg atgagccgat
     7321 gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg ctctgtgtca
     7381 gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc gtcatgcgca
     7441 tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag aagaggtgct
     7501 cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg atatcgaagg
     7561 tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc gggtaattac
     7621 gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt
     7681 attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc gccgccaaat
     7741 gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct acaaagctgg
     7801 catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg ttcgggattt
     7861 gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc cccgagaaac caaagaacaa
     7921 gcctctatcg gcacgcaaat tgataaagac gagttgctgc ctggtgacct ggtctttttc
     7981 aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa caaccaattt
     8041 atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt ctattggcag
     8101 aaaaatttct ggcaggcgag acgaatttag tatgtcgaaa aatggacggc gatgatacat
     8161 cgccgtccat ttactcctga atacagtagt attttgcaga caaaaattcc gtaaatggat
     8221 tattcgttta taatcaggat aatctaaaaa aaggacccct gaaaaagtgg ttaacatgga
     8281 acaaatgaaa gttactcctt atgtaattgt tatgtcttat ggcctcatcc cttttagccg
     8341 gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt ggagaatctt
     8401 tatcattctg attgctattt cctgccgata agagataacc agcaagacct tgtcggcgtt
     8461 gagttaatta cccatttctc cagtgaagat ggtacggttc gtatcccgac gagtcgggtt
     8521 atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt ggaattacta
     8581 aaatcatgcc aacatttttt tattcaacat aaactctttg cctggcttaa tttaacgcca
     8641 caagtggcta cattattatt agagagggat aattacgcag gcgaactatt aaaatatccg
     8701 tttattgaac tgttgattaa tgagaattac ccgcatctca atgaaggaaa agataatcgc
     8761 gatcttttat ccttatcgca aatgtatccc cttgtgttag gcaatttggg agcaggtaat
     8821 agcacaatga aagcggtttt tgatggtctt ttcacccgcg tcatgctgga taaaagtttt
     8881 attcagcaac aaatcacgca tcgctccttc gaaccgttta ttcgtgcgat ccaggcgcaa
     8941 atttcccctt gttgcaattg catcatcgcg gggggcattg atacgacaga aatattggca
     9001 cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc cgttcctata
     9061 aatcagataa cgacgttggt tcagcgataa ccctcctgtt tgctggtgtt taagacgaga
     9121 gtaaccgtct acactattaa acaggaggat ctatgaccct gtcttttatt acccgctggc
     9181 gcgatgaatt gccagcaacc tatacagcac tttcccctac gcctttaaat aatgcccggc
     9241 tgatttggca taataccgaa ctcgctaaca cgctgagtat tccatcgtcg ctgtttaaaa
     9301 atggcgcagg tgtctggggc ggcgaaaccc tactgcctgg tatgtcacca ctggcgcagg
     9361 tttacagtgg tcatcagttc ggcgtctggg cgggccaact gggtgatggg cgcggtattt
     9421 tactcggcga acaactgctt gctgatggca ctacaatgga ctggcatctg aaaggtgctg
     9481 gcctgacgcc ttattcgcga atgggtgatg gacgggcggt tttacgttcg acgatacgag
     9541 aaagtctcgc cagtgaggcg atgcattatc tgggcattcc gacgacccgc gcgttaagta
     9601 tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga gccaggcgcg atgctgatgc
     9661 gtgtggcacc aagtcatctg cgctttggtc atttcgaaca tttttactat cgccgcgagc
     9721 cggaaaaggt tcgtcagtta gctgactttg ccattcgtca ttactggtca tatcttgaag
     9781 atgatgagga caaataccgc ctctggttta gcgatgttgt cgcacgtacc gcatcgttaa
     9841 ttgcccaatg gcagacggtc ggctttgctc atggggtgat gaataccgat aacatgtcgc
     9901 tgctggggct gacgcttgat tacgggccgt ttggttttct ggatgattac gaacccggtt
     9961 ttatttgtaa tcactcggat catcaagggc gttatagctt tgataatcaa cctgctgtcg
    10021 cgttgtggaa tttacagcgt ctggcgcaga cattgtcacc atttgttgcc gtagatgccc
    10081 tgaatgaggc cctggacagc tatcagcagg ttttgttgac gcattatgga caacggatgc
    10141 ggcagaaact gggcttcatg acggagcaaa aagaggataa cgcgctactg aatgaattat
    10201 tcagtctgat ggcgcgagag cgcagcgatt atacccgcac attccgcatg ctgagtctga
    10261 ccgagcagca cagcgcggcg tcaccgctac gtgatgagtt tattgatcgt gcggcatttg
    10321 atgactggtt tgcccgttat cgggggcgtt tgcaacaaga cgaggttagc gatagtgagc
    10381 gtcagcagct gatgcaaagc gttaacccag ctctggtgtt gcgcaactgg ttggcgcaac
    10441 gggcgattga agcggcagaa aagggtgata tgacggaatt gcaccgcctg catgaggcgt
    10501 tgcgaaatcc tttcagcgac agagctgatg actatgtcag tcgtccacct gactggggta
    10561 aacggctgga agtcagctgc tcgagttaaa ggctacttgg tcaacagcag cttgccagcc
    10621 tgagttttac ggagcagata ttcttgcccg tcatgatcga taatcagttt gccatccgga
    10681 cctaacagtg tctggctgga aattcgccga ataggctgcg gggaagcggt cttgtgattg
    10741 gcatccgttt caggcgtgag ttttctgcta tccgtataac gcataaaaat taataataat
    10801 tgatataaca atgattctta ttatcaatat tatggctgac cagagcaagc agtaatgtgc
    10861 tgagaagaca acaagttgcg cagaaatgcc catcccatca ggaatgggca cgcattcaga
    10921 agcgggtatc taccgcagag gcaagttttt cgaccaggcg ttcggtatct tcccagccca
    10981 gacacgggtc ggtaatggat tgaccgtaag tgagcggctg accgccgacg attttttgcg
    11041 ttccttcgcg caggaaactt tccgccataa ttccagcaat cgccgtagag ccattgcgga
    11101 tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc tggcagttac
    11161 cgtggctgaa atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc gtatcgcagg
    11221 ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata ataatgtggc
    11281 catacgggtt gccgctggtc tgatagatgg tcatctgacc atttttgtct ggcgagagaa
    11341 acatatggct ggcgcgggct gcgcggatag catccacggc aatccgcgag ttgccatcgg
    11401 taccattttt aaaacctacc ggacaggaga gtgccgaagc catttcacgg tggatctgac
    11461 tttcggtagt acgtgcgcca atcgcgcccc aactgattaa atcagcaata aactgaccgg
    11521 tcaccatatc gaggaactca gtcgcggtag ggacacccag ctcatttacc tgtaaaagta
    11581 atttgcgcgc catctccaga ccgtgattta cccgatagct gccgtttaaa tccggatcgg
    11641 agattagccc tttccagccg acaacagttc gtggtttttc aaaataggtg cgcattacga
    11701 tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg tactccattg
    11761 cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc ttatcttcac
    11821 cattcagtat tttttcaatt ctgcggcggg agtcggtgac atgggtggcg acgccaggcg
    11881 ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata cgcgcagtac
    11941 ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta cggcaggact
    12001 accgggaagt gatgcgcatc acgataaacc aatcgcagct gatttcaagt gcgaggtgtg
    12061 tcacacctcg ccgggtggtt agcatgataa caaaaaaata agctaatgca ctagttctct
    12121 agtacattcg gcggctaagc cccatgatat cgaggagctt ggtggcaatc tcttctaccg
    12181 aataattggt actgttaatc cacgggatct gatttttacg gtacaacgct tctacttccg
    12241 cgacttccat cctgcactga cgaagcgagg catagcggct gttctcccga cgttcctcgc
    12301 gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc tgaagcggtt
    12361 tgagcgacgc gggtagcacc agattgtcca tatcgtcggc aataaagggg tagtttgccg
    12421 cgcggatacc aaactgcatt gccagataca gactggtggg ggttttacca cagcgagaaa
    12481 caccgagcag gatcacctga gcctggtcca gattgcgcaa cgaaatgccg tcatcgtggg
    12541 cgagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta ttagggttaa
    12601 ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt tgtagcgggg
    12661 caaccagcgc ctgaacaata tcctggcaaa agccttcact ttgcaagatg atggcgcgaa
    12721 tctccggcaa cacgatggag tagaagacca gcgggcgcac acctgtctgg tgataaattg
    12781 cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac ggcagcgtaa
    12841 tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc tccgcagtta
    12901 ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc attttgttct
    12961 tcccgtgatg cagacataat tctatgaaag cataaattaa aaacgcacag aagcgtagga
    13021 cgtaatgtct ggtttataaa atgaaccttc aattttattt tttatgaaaa cagcatttca
    13081 tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaatag agcaggtttg
    13141 cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac gattcaccgt
    13201 ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc tcaaaccgtt
    13261 catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct ggtgctttgg
    13321 tataaccaac tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc ctccctgggt
    13381 gaaatgatta ctaacctttc cggaatgggt gtttccgttc cgaatggttt cgccacaacc
    13441 gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat ttatgaactg
    13501 ctggataaaa cggatattga cgatgttacc cagcttgcga aagcgggcgc gcaaatccgc
    13561 cagtggatta tcgacactcc cttccagcct gagctggaaa atgccatccg cgaagcttat
    13621 gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc cgctactgca
    13681 gaagatatgc cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa cgttcagggt
    13741 tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cgatcgcgcc
    13801 atctcttatc gtgtgcacca gggttacgat caccgtggcg tggcgctctc cgccggtgtt
    13861 caacggatgg tgcgctctga cctcgcatct tctggcgtga tgttctccat tgataccgaa
    13921 tccggctttg accaggtggt gtttatcact tccgcatggg gccttggtga aatggtcgtg
    13981 cagggcgcgg ttaacccgga tgagttttac gtccataaac cgacacttgc agcgaatcgc
    14041 ccggctatcg tgcgtcgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc
    14101 caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc
    14161 tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac
    14221 tacggtcgcc cgatggatat tgagtgggcg aaagatggcc acaccggcaa actgttcatt
    14281 gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg
    14341 cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggcgcgggt
    14401 ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg cgacgtgctg
    14461 gtcactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatcgtc
    14521 accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggcattccg
    14581 gcggtagtgg gctgtggtga tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt
    14641 tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa
    14701 agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac
    14761 ccggaccgtg ctttcgactt cgcctgcctg ccgaacgaag gcgtgggcct tgcgcgtctg
    14821 gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat
    14881 caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt
    14941 gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg
    15001 aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt
    15061 ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc
    15121 tatgtttccg acagcttccg cgactgcttc gcgctggagt gtgaagcagt gaaacgagtg
    15181 cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg taccgtagat
    15241 caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaaacgtgg cgagaacggg
    15301 ctgaaaatca tcatgatgtg tgaaattccg tccaacgcct tgctggcaga gcagttcctc
    15361 gaatatttcg acggcttctc aattggctca aacgatatga cacagctggc gctcggtctg
    15421 gatcgtgact ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc ggtgaaagca
    15481 ttgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt
    15541 cagggtccgt ccgaccatga agactttgcc gcatggttga tggaagaggg gatcgatagc
    15601 ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga actgaagaaa
    15661 taaaataaat ccccggcggc gttcagtcgc cgggtttatg tgatccccga agataaaact
    15721 tattcaatct cttcacagac atcctgcgtt aatcgccgca taatatcttt tcttaacaaa
    15781 aacttttgta ttttacctga ggcagtgcgc ggtagttttt cgattaccac gatatgttca
    15841 ggatatttat attttgcgac ccgtttacgg ctaaaaaaag ccaccacctc ttccagcgat
    15901 aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc taaacgttca
    15961 tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa aatatcttcc
    16021 acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt gcgtccggta
    16081 atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata ccagccttct
    16141 tcatccagcg cacgggcggt taattcaggt tcatcaaaat atcccataaa cacattgggg
    16201 ccacgcgagg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc gtcatcgacc
    16261 actttaatct ctacacctgc ggcagcgtaa ccatcggtgt gcataaagcg cgacaaagga
    16321 tcatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac acttaataat
    16381 ttaatgccga gctgctggca ttcacgcgcc acttttttgg ggattgtggt tccgccgcaa
    16441 agaaagaaac gcagcgctga caggtccgcg ggttgtttct ctagtacatt caaaagatca
    16501 tagacaaacg gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag cagcgcgaga
    16561 cacgcatcag gagtgaaaat atctaacaac acgctgcgag cgccaattaa gaatggtgcc
    16621 gttacgccat gcagaaagcc cgttgcgtgg ccgagtggcg caggcatcat aaagacatcc
    16681 tgccaggtca gattcagccg cgcgcaataa gcccgctcac tggcgagaat attgttatgc
    16741 gttagcatca cgccctttgg cagaccctcg gttccggagg taaacagcac cgcagctaat
    16801 tcatcgccgt gggtcgttat cgccgtggtc agtggggtat tgtcggcgat aatctgactt
    16861 aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg ttgtagttgt
    16921 ggaagctgat tttgcagcgg caggattaaa tctaccgggc gcgtttgttt aaacaacgtc
    16981 ggtgcaaaga acatttttgc ctgacactta ttgagcaccc acaccagttc tgcttcccgc
    17041 caggaaggca acagcggcac ggaaaccgca ccgattttca ggcaggcaag atagataacg
    17101 gtaaattcac accagccagg cagttgaaat gcgatgcgat cgcctgattc aatacccttc
    17161 gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata ggtgtacgat
    17221 gcaccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt ctgctgccag
    17281 taatcggcca gcgaagcatc gccccatagc ccttgctgac gatacgccgc acgacgttgt
    17341 tcgttgaacg ttaatgtcac tttcatgttg gactctcgaa acagaacatc aggcccgaga
    17401 tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag gtgcctgcgg
    17461 gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg ccgcactcca
    17521 gacaaccggc agaatcaaaa tgaatgttgc cggcgtcatc ctgcttataa agtccggcag
    17581 ggcaggcttt cattaattta cggaattcat tgatatcggg atttgccgcc agaatgatat
    17641 gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg acgttaaccg
    17701 tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca agttgatcag
    17761 cccaattttc ttcgcatgtc ccatgatcat tttgcgtacc ggctggtttg gtttgccgtc
    17821 aatggtgaac atctcgttca tgatgtcggc gaccattcgt gggtattggc taaacaggcg
    17881 cgggttttcc atcagcgccg ggatcttgcg gaaatgctgc atatcgcgca tgatgcagct
    17941 ttgttccagc tcacgtttgt attgcgccag actgctggcg gagaaatcct cgcgttcttt
    18001 ggcggcgatc accgttgtgg cggcagcctg agccgatgca atggctaaat ccatgccacg
    18061 aactgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca tcacgccatc
    18121 gttaaccatc tgcggcacca ttgccagacc gccttctggc accatatgcg cggaatattc
    18181 aagcagtttg ccgccgctaa ttagtgggcg aatggcgggg tgttgtttaa aatcttccag
    18241 catttgcggc acgctttttt gcgcatgggc gatatcaccc aatccacaaa ccagccccaa
    18301 ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt cagaagggga
    18361 accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat cgttgatctg
    18421 ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg gatcggaagc
    18481 gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcagcca gaatcaccac
    18541 attcgcttcg agaatatcat ccccagcctg cacgccagtg accttgtttc cttcacgaac
    18601 cagcgcatcg acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg cttgttccat
    18661 caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgcg gaacatctgg
    18721 ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg gttaagaagg agattttctc
    18781 gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag cctggaatga ttgcttcaag
    18841 tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt cgccgcgttc
    18901 tatcaccagg acatccagcc cggctcgcgc catgaccagt gcggcaacgc taccagcaac
    18961 gcccgcaccg accacaatgg catcaaattt gtcttccgac atgcgttatg ctcccttccc
    19021 gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac tggctaatca
    19081 gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcataa ttgaagattg
    19141 gcgcattttt atctttattg atggcgacaa tcacttttgc gccgttgccg ccaaccatat
    19201 gctggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc aacacgccgg
    19261 agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt ggacgtgaac
    19321 agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg tcatcctgcg
    19381 ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc acgctgcttg
    19441 cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaaca taaggtaccg
    19501 tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca ccgggtgcaa
    19561 gggtaatgat cgccagcggg ctgttgatct tttcctgagc gaacgctaac ccgccataca
    19621 tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca tcgttcacca
    19681 gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg
    19741 ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt tcggcgtaat
    19801 tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt ggaccataag
    19861 gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt tggccccatt
    19921 gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag acccagacgc
    19981 tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt cgtcagatgt
    20041 tccgccagct cggcaatggc ctccggcgaa tcgttatcga taatgatgtg cttacgttct
    20101 gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg gctccagtca
    20161 atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat ggctttcatc
    20221 gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg aacagagagt
    20281 tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc
    20341 tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac cagcaagcca
    20401 acctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc gaagccgatc
    20461 ttttcaattg ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc
    20521 gcatcctgca ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc
    20581 gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc atctgttgcg
    20641 agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc gtcggcattg
    20701 tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg caccagctta
    20761 aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca tctccgatgg
    20821 aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggttttcg acggagcaag
    20881 ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatc gctaaaacaa aagtgcaatt
    20941 tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt
    21001 tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat ccggcatata
    21061 ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt tttgataacg
    21121 ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt gcatttagcg
    21181 atgatccaaa atgggagtcc ggacatcacg ttcatgacaa tgaaaccgag ttgatttacg
    21241 tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt atatgtcgca catgcggatg
    21301 acattgtggt gatagaacgc ggcaggctac atgcggtggc ctctgacgtt aacgatccgg
    21361 caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag gaaaatcagc
    21421 tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa gtcataaaaa
    21481 ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa acatcttcgt
    21541 tatgggatgc atttgcctat acgttagcca ttctttacta cgaaaatttt aaaaatgctt
    21601 atcgttcgga gcaggggtat attaaaaaag atgttctgat aaaagatatt cttttctatc
    21661 tgaataataa ttatcgcgaa aaaatcactt tagaacagtt atcgaaaaaa tttcgtgcca
    21721 gcgtcagtta tatttgccat gaatttacca aagagtatcg tatttcccct attaactatg
    21781 ttattcaacg gcgtatgacg gaagcaaaat ggtcactcac taatactgaa ttatcacagg
    21841 cagagatctc ctggcgtgtg ggttatgaaa atgtcgatca ctttgccaaa ctgtttttgc
    21901 gccatgtcgg ttgttcgccc agcgattacc gtagacaatt taaaaactgt tttgcggaac
    21961 aagaaatcct atctgaattt cctcaaccgg taagtcttgt tggataaatc tggcggcgca
    22021 gtttgttaag aagctgcgcc gcctgcagat tatttgttct gatagtcttt caggatctgc
    22081 cgacctgcta cgtaaatcat aatttcgtct gtaccgccgc cgatacgttc acaacggaca
    22141 tcacgccaga agcgggagac gcgcgcttca tcggtatagc ccagaccgcc catgatttga
    22201 atcgcatcat caatgacttc cattgcggta cgtgcgcaat acagttttgc cagcgccgcg
    22261 ctggtgcgca gtgactgatg ctgatcggct tgccatgcca ctttcagcac catgttgcgc
    22321 atgttgtcga tcttaatcgc catcagcgcc agtttttcct ggatcatctg gttatgacca
    22381 atgggcttac caaaagcgat acgttggttg gcatagcggg cggcatcttc aaaggcgcat
    22441 tcggcaaagc cggtgctgcg cgcggcgttg atcaggcgct ccatctcaaa gttgtacatc
    22501 acattgagga aacccattcc ttcttcgccc accatgtcgc tctcttcaac ttccacattg
    22561 tcgagataga cttcgcaggt gctgagcata tgccagccga ttttatgcag cgggttaatc
    22621 ttaatgccgg gcttactgga gtcgacccac cacagggtga aggctttttt gggatctttc
    22681 ggttgcggat cgcgcgccag caccagcata tacgggtact ctttcgcact ggtaataaag
    22741 gttttttgtc cgttgatgta gaccttgccg tttttacgcg tgtaagtggt agtggcactg
    22801 ttgttatctg agccagcacc tggttccgtc aacgccaggg cataggcggg atcacccgtt
    22861 tccagggtgc tctctgccgt tttacgtagc tgctctgcag aaccgaaacg gcgcatactg
    22921 tgaatacatt gaccgttggt aattaaaaat gccggagcac cgcattttga cacttccatc
    22981 agcgccagca tttgggtgac gtaatccgca gggataccac caaattcttc cggcacgcca
    23041 agcatggaaa taccgttatc cgccagcgcc cgcataaact cacgcggata tgtcccgttt
    23101 tgatcgcagg tgcggaaata ctcttccgga aagttagtcg taatcagttc gcgaatactg
    23161 gccagcagca gttcttgttc ttcagttaaa gaaaaatcca tcattatact cctgttaatg
    23221 agccgcttca ggcaggacaa aacccatcgc cgcatcgata aataatcttt cgtccatcag
    23281 tttgagttct ggcgaaatca ctggggtgaa gtccattttg tcgagaatat ctttttgtaa
    23341 atcgacgcca ggggcgattt cgattaaatg caggccgtct tctttcagcg tgaatacagc
    23401 acgttcggtg atgtaacgaa catccagtcc ccgttcgagc gcgatttttc cgctaaaagt
    23461 aatttccggt agttcccgaa taaatttatt tacccgtcct tcctggacga tatgcaattt
    23521 gccatcggca atttctgttt ttaaactgcc cgcagttaac gtgccgcaga aaataatttt
    23581 cttcgaggtg gcactgatat caataaatcc accggtaccc atgattttgc cattgaattt
    23641 atgcacgccg acgttaccgt gctggtcgac ttcagcaaaa ctcaaataac aaacatccag
    23701 accaccaccg tgataaaaat caaactggga cgtcatatcc agaatggcgc gggtattcac
    23761 gttcgcgcca aaggcgatac cctgtgaagt aattccgcca atcggacctg tttctaccgt
    23821 cagaataaag tcatcagcac aaccttcttc ccgggcgacc aggccaatac cgtcagcaat
    23881 accgacgccg acattcccca ccgcgccttt acgcatttcg aataatgcac gccgcgcaac
    23941 taatttacgt tggtttaggg gcaggctaag tttgtgctgt catcaagggt gaagtcacca
    24001 gaaataaaac ggttaaccgg cgcaccgcca tacagttggc tttgatccgg atcgaccacc
    24061 acaatatcca ccagataacc cggaatacgg acagatttag gatgcagcgt ggctttctta
    24121 accattttct gcacctgcat catcacaata ccgccgttat tgtgcaccgc ctgggcaata
    24181 accaatgcgt cgagatacat cacctcatct tcaaaagtgg cgtagccttc actgtcgcag
    24241 gtggtagcgc gaatgaaggc gatatctggc gcaatcgctt tgtaatagag atattctttg
    24301 ttatcaaact cgaccagttt aatcaggtct tctttcgtga cttcattcag tttgccgcct
    24361 tgctggcgtg gatcgacaaa tgtcccgatg ccaatatcac taataatacc aggctggtgg
    24421 gctgcggcgg cgcgtaaggt ttgtgtaagt acaccttgtg gatagttata agcaataatt
    24481 ttattttgtt ctgcgagttc agaaatacgc ggcgattgtc cccagtgacc acataatgcc
    24541 catttcacca gaccttcttg cgccagagga ctaataccac ggtcggcgcg atcgccaagc
    24601 cctgtcggac taataatcga taaattacgt ggcgtttgag tctgtttata tttatcagca
    24661 agagctgtaa ttaacgtggt ggcttccaga ataccgccgc cagcgcctaa cacacaaagt
    24721 gttgcttcgt cgggaatata attcaccgct tcctgtgccg acaggaccgg cactcgtcca
    24781 ttaatacgag gtggttttac aggtttcatt gttaattcct gattcagatg tgattttccc
    24841 tacgccagat ggagaattag cgcacagaga ctcacgaaat atttttatta aatacggtta
    24901 aatatatttt ttaagttcgg cggggagggt gttcccgccg aaatattatt gcttatgcct
    24961 ggtgtaaaat agttaatacc gtgcgtaaat catttaccga gatttgccct ggtgcagagg
    25021 ctttttttac cgcaccaaaa gttgccgccg agccaaatac ttcaccagcc agacgagaaa
    25081 ttacgccagt ttttgccatc gacatcgtga tgattggacg atcggcatac tgctcctgca
    25141 tctccagggt cgcggtaagc aacgtcagca catcgctggt actttgcggc atcagcgcaa
    25201 tcttaggaat atcggcgtcg aaggactgca ttttgcgcag acgggcaatg atttcttcgg
    25261 cttccggcgt tttatggaag tcatggttgg acatgacaac tttcacatca tgcgcgtggg
    25321 cgtaggcgac ggtttcttta acctgatcat cgccggtaaa taactccaga tcgatcatat
    25381 caaccaggcc gctgtcgatg gctgcacgat tgagagcaat ataagcctcg gtggaaatcg
    25441 cctgctcgcc gccttctttg gcactgcgga aggtaaacag cagcggtttt tctggcatgg
    25501 tttcacggag aatttttgcc gccgccatga cagactccac attggagagg tcggcaaagt
    25561 ggtccacacg ccattccaga atatcaaagt ccgcttcacg ataggcgaga gcttcggatt
    25621 tcacgcgggc gatatctttc gccatcagcg agacgatgat tttaggtgcg cccgtaccaa
    25681 tgacgagatc ttttacagtt acggttttca ttttttaccc tttctgcacg cggtcagcct
    25741 gtcaggcacc gaaccccatg acctgtttaa catattccag agggaaatct ttgccagtcc
    25801 ataacgtgaa ctgttcagcc ccttgccaca acaacatgcc gtatccatca atcgttttgc
    25861 aaccagcttg ttgcgcctgc tgcaataact tcgtcatatg cgggttatac acgcattcag
    25921 tgaccagcag tcccggatgt aacagactga tatcattaac taatgattca ttctcaaggg
    25981 gtttcatacc cacttttgtg ccattggtta aaatgtcggc ggaagccagg gcttcagcaa
    26041 aggcttgctg atcggcgaga tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc
    26101 gctgcgcgaa ggcgagggct ttatcgaaga actcatcccg acggttaaag agtttaattt
    26161 cttttaaacc ttcaattgcc ccctgcgcgc caatcgccgt tgaggcacct ccggccccta
    26221 acagcaccat tgttttgcct ttgatatcaa aaccgctctc tttaatggcg cgaatatggc
    26281 ccgtgccgtc ggtgttatag ccgcgcaggt agccatcatc attaacgatg gtgttgatgg
    26341 cacccaccag tttggctgct ggcgttaatt catcaacata ttcacacgcc agttgtttgt
    26401 tcggcattga tataccagtt ccgcgcattt tgagggcttt taatccttca attgctccgg
    26461 gaaaactatc gttatccact tcaaaggcca tataggtaaa tggcaatccc gctttttcta
    26521 aggctttatt ctgcatttca ggcgataaac tgtggcggat aggataggcc atcagcccaa
    26581 tcaattcgta ttttgcggta acatccataa ttaactcctt taacctctat gcttaattgt
    26641 gtttaaccgg gtgctaaatt tacgctcgcc aaaccgcaca tcattctcag gaataataaa
    26701 aacgcggtaa tagcggataa aaacaataat tgcggtaatc agggccagca gcgcgaaagt
    26761 aaaatcgaga acaatgatat attgaaggcc gatgttcgac agataaccgg taatcagcgg
    26821 aataacaaag ttagccagtc cacccatcat catataaata ctggtgactt tggctttgct
    26881 ttttgggaaa aactctgaca tgaccgaaac accgagctgt aaaatgccgc cagctgctga
    26941 gaaaccgata acaaaggcgc cggcattaca caccagtgga gaagggtaca ggtagataat
    27001 ggctgctgtt attgttgcca gtgcagagtt aaatacatta gcccagatgg gccggaccat
    27061 ttttttcagt agtgcggcaa aaataaagac acagaccaac gagcccatac tgtaataaga
    27121 gatggttttt aatgcctcag cttctgacat accagcaaaa gccatcgcat atttgggcat
    27181 ccacaccaca atcacataaa aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac
    27241 accttccagc cagactaacg gtttgctgtt catttgcggt aattcattag ctacgctggc
    27301 gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca gtacaaacag
    27361 aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca tatagctcac
    27421 cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta tgaccgccga
    27481 accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat ccagcgctga
    27541 gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg
    27601 aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg cccgacgacc
    27661 aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca aaccaatacc
    27721 ggaaattaag taggcaatgc ccgcgttgtc agtggaaaac ttttccgcca gagatgacat
    27781 gttttgggca agcgtaataa cactaatgcc gtgcaggaag tagctgaagt aaatacaaag
    27841 aacagccagg ataaatggcg tgctgaaagc cttattttga gacattttca cagctccttg
    27901 ctgaaagata atttcgttac cgtacgtatt gttcttcgac ggtttacata attactatac
    27961 tgaagatgat tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt
    28021 catagtttgt ttttgatcgc gattttcctt agttagcgca gaaagagtta tttatccgta
    28081 agatcttgca tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta
    28141 gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac
    28201 cgatggcagc gatggcggta tcgaaccaca taatatcggc aatacttctt tgggacagat
    28261 gagccgtaat caacggaata gtaaaggtcg caatactgcc cgcactgtaa tagatccctg
    28321 tagctttacc tttagcgtaa gggaaacgtt cagccattaa cgtcaggcca atttgcacaa
    28381 cacctccagc agaggtaaaa ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg
    28441 gatgcaggca gacggtaaac agagcaataa atgagataaa ggtgtacagc atcagtaatg
    28501 ttgttgggcg aacggtatta cgaatgagtg gagcggtaat aaatacacaa agcagcgaat
    28561 ccacggtgta gatactgagt agtttaatcg acatagtgta tgacatgcct gcaacaaatt
    28621 gtccgtactg tgccagccac tggctaacca gataaaacgt tgccattgag atatagccat
    28681 ataaggtata actggctaaa tcgataattg aacagcgatg ttccgtagag ctggtggttt
    28741 tctttatgac aggtaagcga tgacccggat ggggtgggaa cgtacaacgg tataaaaaca
    28801 gagcgttaat aaacataatg cctgcagcaa tcataaagga ccaaccgaac cacagttcag
    28861 cccacactaa caggctaatg attagcggta ataaaaattg tccgctggaa acaaatgctt
    28921 taattaaaat attggctgtc ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag
    28981 tgcctgcatc gagaaaactg tttgccattc ctgccagaaa gccaaaaaca taagcgataa
    29041 tgatgttatt ggtgtgcagg atgccaaaaa agaaggccat atagcagcac atcccgagca
    29101 tgataaaagg gcggcgacca aagcgatcgg ataataatcc tgcaaaaagc aagacactta
    29161 atcgaccaat gcccagcgat gagataacta tcgagacacc cgcggcatta gtctgccaaa
    29221 gtgtctccag cgaggccata ttcaggctca tcaaaatgac gcccatacca tgcactaggt
    29281 aattaaaata caacccaagt gcggtagggt aatagggatt tttcataggt tctacttagc
    29341 aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc cgttcattat tgttgtaaca
    29401 acgacaattc ctttttgtgg agaaattaac aaagccgctc cagatcgtgt gcatataatt
    29461 cagcggctac cgattgatag atcttccagt caataaaatt gccatcaggg tagacttgtt
    29521 gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt
    29581 tctctatgat agcatgaaga tgtttttttc gttgctgacg catagccaac agttgctcga
    29641 ctacacgatc aggaatggcg catttgccat tttcccactg acgccagctt tcactgtcac
    29701 cggtctgggc aatccaggtc gcgcattcat caatagtcat ggcaaaaata tggcgaagtg
    29761 cctggagttc ataagcgttc atcgtgtaat ggctgcatga aataaacaat aaaatataaa
    29821 taacattatc agcctgctga cggcttgaag agagtcacag tatcttgtgc aacattattg
    29881 gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga
    29941 gatgggcaaa gactacacac agcaattcgt tgtttcactc aggggatttc catgcttata
    30001 aatcaatatg ttgatttata accgtaagct aatagtaggc atacggactt tttgcgtcga
    30061 tcagattcgt ctcattagtt taaaaactgt aaagaatttg cgacaggtac taccagctga
    30121 atggcggtaa gcaggaaaat atggggaaga gcaagcaaat atcaattagc gggggttaac
    30181 aatcaggcgg cagagccgcc tgatgagaaa ttacttattc ggtttctcga tttcgccaag
    30241 ttcttcgaga atttcttccg gttggtccgt cggcggcggg acttcttcca cccacgcggc
    30301 aaacagacgc caggaaacgg ctaacagaac cggaccaata aacagaccga tcatcccgaa
    30361 agcaatcaaa ccaccaataa caccagagag gatcaggatc agcggtaaat cggcacccat
    30421 gcgaattaac attgggcgga tgacgttatc cagcgtgcca accacaccgc tccacactaa
    30481 caataccgtt ccccaggtgg tatcgccagt ccagtagagc cagataatcg ccggaatcag
    30541 taccggcaac gggccaagct ggacaaggca ggagaggatc attaacaccg ttagcaacgt
    30601 tgcataaggt acgccggata cggcgaggcc gataccgcca agcactgcct gtactaacgc
    30661 cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt gccgccagca gtacggcggc
    30721 atctccgcga acgcctgcca gacgggttgc aaaatggcga atgccttgtg ccacctgttc
    30781 accgcgccag tacagcaggg cactgaagag aagcatcagc gcacaatgca ccataaagcg
    30841 cccgatatgc gccgcctgcc caacgaacca ggtggtggtg gtgccaatat aagggcggac
    30901 tttcgccatg atcgccgtcc cccccatatc cagcaagttg tgccagcctg catacagctt
    30961 cgcgccaatc accggaatgg tattaagcca cgccagatcg ggtaacgtca tgtcgccgct
    31021 ggaaatggct ttaattagcg ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat
    31081 agggatgata aacaccatca ctaataacag cgtcatcacc agaacggcga gagagcggcg
    31141 gccaaacatg atcttttgca aacgtaacaa taccggccag gtggcgataa ccaccgtacc
    31201 ggcccatgca aagccgagaa taaagggttg aacaatccac agacatgcca caatcatgat
    31261 ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac gaacatttac
    31321 cataactact tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa
    31381 ctcacctgca taatgatgag caatttcagc ggtttttaac aggccgattc tgcccttaat
    31441 tctgatgggc gtgttagaaa aaaatgtggt acaacaactc aggcaacacg caaacgattt
    31501 actcgtcgta tttcaagttg catgtgctgc ggctacgttc gttcacccca gtcacttact
    31561 attgtaagct cctggagttc actcgcttgc cgccttcctg caactcgaaa tccatagagt
    31621 aaataaacat cacctttcag tgcgctattg cagccgcctg aaagaatagt gtatcgacct
    31681 acatcacaga ccgcaggaaa gggtcaatat aatgattcca cagatttccc aggcacccgg
    31741 cgtcgttcaa ctggtgctta attttttgca agagctggag caacaaggtt ttaccggcga
    31801 tacggcgaca agttatgccg atcgtctgac aatgtcgacc gacaacagta tttaccaact
    31861 tctccccgat gcggtggtat ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct
    31921 tgccgcgcag gaacgctatt catcgctgat ctttaccccc cgtggcggcg gcaccggcac
    31981 taacggtcag gcgctcaacc aggggattat tgttgatatg tcccgccata tgaaccgcat
    32041 catcgaaatt aaccctgaag agggctgggt gcgcgttgag gccggggtga taaaagatca
    32101 actcaatcag tacctgaaac cgttcggcta cttttttgcg ccggaacttt cgaccagcaa
    32161 ccgggcaacg ctcggtggga tgatcaatac cgatgcttct ggtcagggat cgctggtcta
    32221 tggcaaaacg tcggatcacg tacttggcgt acgtgcggtg ttgttggggg gcgatattct
    32281 cgatacgcaa cctttacccg tcgaactggc ggaaacgctg ggtaaatcca ataccacaat
    32341 cgggcgaatt tataacacgg tttatcaacg ttgccgtcag caacgccagt taatcatcga
    32401 caacttcccc aaacttaacc gctttcttac cggttacgat ctgcgtcatg tctttaacga
    32461 tgagatgacc gagttcgacc tgacgcgcat tctgacgggt tcagaaggga cgctggcctt
    32521 tattaccgaa gcgcggctgg atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt
    32581 caaatatgac tcttttgact ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc
    32641 gctttcggta gagacggtgg actcaaaagt gctgaatctg gcgcgggaag atattgtctg
    32701 gcattccgtc agcgagttga ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat
    32761 tgtggagttt gctggtgatg atgaggcgct gattgacgag cgagttaatg ctctctgtgt
    32821 gcggcttgat gagctgattg ccagccaaca ggcaggtgtg attggctggc aggtgtgccg
    32881 cgagctggcg ggtgttgaac gtatctatgc gatgcgtaaa aaagccgttg gtctgcttgg
    32941 caatgccaaa ggtgccgcta agccaattcc gtttgctgag gatacctgcg taccgccgga
    33001 acatctggcc gattatattg ctgaatttcg cgcgctgctc gacagccacg gcttaagcta
    33061 cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc cgtccagcgc tggatatgtg
    33121 cgatcctcaa caagagattt tgatgaagca aatctctgat gacgtagtgg cgctgactgc
    33181 gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg aatacagccc
    33241 ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc
    33301 gcataaccga ctcaacccag ggaagatttg cccgccagaa ggtctcgatg cgccgatgat
    33361 gaaagtggac gcggtgaagc gcggtacatt cgatcggcag atccccattg cggtacgcca
    33421 gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta tgcttcaact ttgatgcccg
    33481 tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg attcattcac cgaaagggcg
    33541 cgcaacgctg gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg atccactcaa
    33601 actggaacaa gaactgcctg aatccggcgt cagtttgcgg acgttaattg cccgcacgcg
    33661 caatagctgg catgcgaata aaggcgaata tgacttctca cacgaagtca aagaggcgat
    33721 gtcgggctgt ctggcctgta aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc
    33781 ggagtttcgc tctcgttttc tgcagctcta tcacacccgt tatttacgcc cgctgcgcga
    33841 ccacctcgtc gctacggtcg agagctacgc gccgctgatg gcacgcgcgc cgaaaacctt
    33901 taacttcttc attaaccagc cgctggtgcg caaactctcg gaaaaacata tcggcatggt
    33961 tgatttgccg ctgctgtcgg tcccctcgct acaacaacaa atggtggggc atcgctcggc
    34021 aaacatgacg ctggaacagc ttgaatctct caatgcagag cagaaagcgc gcacagtgtt
    34081 ggtggtgcag gaccccttta ccagctatta cgatgcgcaa gtggtggcgg attttgtccg
    34141 tctggtcgaa aaattaggtt tccagcctgt gttactgcca ttttcgccaa atggcaaagc
    34201 ccagcatatt aaaggttttc ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt
    34261 cctcaaccgt atggcgaagc tgggtatgcc aatggtgggc gtcgatccgg cgctggtact
    34321 ttgttatcgc gatgaatata aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt
    34381 actggcgaat gaatggctgg caagcgcact tgagtcacag ccagtggcta cagtcagcgg
    34441 tgaatcatgg tatttctttg gtcactgtac cgaagttacc gccttgccgg gtgcgccagc
    34501 acaatgggcc gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg
    34561 ctgcggcatg gcagggactt acggacatga agcgaaaaac cataaaaatt cgctcgggat
    34621 ctatgagtta tcctggcatc aggctatgca gcgactgccg cgtaaccgct gtctggcgac
    34681 cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc acgggggtac gccatcctgt
    34741 gcaggcttta ctggagatta ttaaatgata tggaaacgga aaatcaccct ggaagcactg
    34801 aatgctatgg gtgaaggaaa catggtgggg ttcctggata ttcgctttga acatattggt
    34861 gatgacaccc ttgaagcgac aatgccagta gactcgcgga caaagcagcc tttcgggttg
    34921 ctgcatggag gagcatccgt ggtactggcc gaaagtatcg gttccgttgc cggttattta
    34981 tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca atgctaacca cgtccgctcg
    35041 gcacgagaag ggcgggtgcg cggggtatgc aaaccgttgc atctcggttc gcgtcaccag
    35101 gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg
    35161 accgccattt tgtgacgttg tcaccagaaa agtgtgacgc atattgcctt tggtcattat
    35221 ctgcacagta aacggtggta tactgtgcag gtcttgtcta aaagcgagga tgctatgtct
    35281 actcaacttg atcccaccca gctggccatt gaatttttac gccgtgacca gagcaatctt
    35341 tcacctgccc aatatctcaa acgtctgaaa caactagaac ttgaatttgc tgacctgctt
    35401 actctctctt ctgctgaatt aaaagaagaa atctactttg cctggcgtct gggcgtgcat
    35461 tgatcctgac ttaatgccgc aagttcgcca aatcgacctg gattaacctc cgtccaggtt
    35521 cgatgacctt cttaatactg cttatctacc gcctgatatt gttcaaaaat aatactaatc
    35581 gcagtttgtg ttaaaacggc gggttagctt tatgagatta ttcttatcgc cccttcaaga
    35641 gctaagccac tgagagtgcc ggagataagc gccggatggg gtagaaaccc ttaagcctgt
    35701 gttgcacaga cttaagggtt ttcttatttc tgaacgctgc acttttttac ctatctctta
    35761 aatagtcatt ttcaatacaa ctttttcttt ttcatcacag ccttaatgct ccttagataa
    35821 taaccattat ctaacaatga gatacctaat tcttagcgtg cctgttaacc cacacatcag
    35881 ggtctatgct tattaaaagc aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc
    35941 tgggttgtac tgataatcat tatcactaac atgctgttat acgctgaaag cgatgaagtg
    36001 aggtaaatcg atggacatgc attcaggaac ctttaaccca caagatttcg cctggcaagg
    36061 cttaacgctg acacccgcag cggcgataca catccgtgag ctggtggcaa agcagccggg
    36121 tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc gcgggctttg gctatgtgct
    36181 cgacagtgtt agcgagccgg acaaagacga tctgctgttt gaacacgacg gcgcgaagct
    36241 gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga
    36301 aggacttaat cagatattca aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg
    36361 cgaaagcttt ggggtatagg cggtactatg tctcgtaata ctgaagcaac tgacgatgtc
    36421 aaaacctgga ccggcggccc gctgaattat aaagaaggat tcttcaccca gttagccacc
    36481 gatgagctgg caaaggggat aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat
    36541 gagccggagt ggatgctgaa gtttcgtcta aacgcctatc gcgcatggct ggagatggaa
    36601 gaaccgcact ggttgaaagc gcactacgac aagctgaatt atcaggatta cagctactac
    36661 tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag
    36721 caaactggcg cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga gcagttgggc
    36781 gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca ttttcgactc agtttcggtt
    36841 gccactactt atcgcgaaaa actggcggag cagggaatta ttttctgttc ctttggtgag
    36901 gcgatccacg atcacccgga actggtgcgt aaatatctcg gcaccgtggt gccggggaat
    36961 gacaacttct ttgccgcgct taatgcggcg gtagcctctg acggtacgtt tatttatgtg
    37021 cctaaaggcg tgcgctgccc gatggaactt tccacctatt ttcgcattaa cgcggaaaaa
    37081 accgggcagt ttgagcgcac cattctggtg gccgacgaag acagctacgt cagctacatt
    37141 gaaggctgtt ccgctccggt gcgtgacagc tatcagttac acgcggcagt ggtggaagtc
    37201 atcatccata aaaacgccga ggtgaaatat tccacggtac aaaactggtt ccctggcgat
    37261 aacaacaccg gcggtattct caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac
    37321 agcaaaatgt catggacgca atcagaaacc gggtccgcga ttacgtggaa atatcccagc
    37381 tgcattttgc gcggcgataa ctccattggt gagttttact cagtggcgct gaccagcggt
    37441 catcagcaag cggataccgg caccaagatg atccacatcg gtaaaaacac caaatcgacc
    37501 attatctcga aagggatctc tgccggacat agtcagaaca gttatcgcgg cttagtgaaa
    37561 atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt gcgactcaat gctgattggc
    37621 gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc gcaataacag cgcacaactg
    37681 gagcacgagg caacgacatc acgtattggt gaagatcaac tgttttactg cctgcaacgc
    37741 gggatcagcg aagaagacgc catctcgatg attgttaacg gtttctgcaa agacgtgttc
    37801 tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa
    37861 cacagcgtcg gataaggaat aaacatgtta agtattaaag atttacacgt cagcgtggaa
    37921 gataaagcta tcctgcgtgg attaagcctc gccgttcgtc ccggcgaagt tcacgccatt
    37981 atggggccaa acggttcggg caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat
    38041 tatgaagtga cgggcggcac ggttgagttc aaaggcaaag atttgcttgc actgtcgccg
    38101 gaagatcgtg caggcgaagg catctttatg gccttccagt atccggtgga gattccaggc
    38161 gtcagtaacc agtttttcct gcaaacggca cttaatgcag tgcgcagcta tcgcggccag
    38221 gaaacgctcg accgctttga ttttcaggat ttgatggaag agaaaatcgc tctcctgaag
    38281 atgccggaag atttattaac ccgttcggta aacgttggtt tttccggcgg cgagaaaaag
    38341 cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt tatgcattct tgatgagtcg
    38401 gactccgggc tggatattga cgcattaaaa gtggtcgccg atggcgtgaa ctcgctgcgt
    38461 gatggcaagc gctcattcat cattgttacg cactaccaac gcattctcga ctacatcaag
    38521 cctgattacg tccatgtgct atatcgggga cgaattgtga aatccggcga tttcacgttg
    38581 gtcaaacaac tggaggagca gggttatggc tggcttaccg aacagcagta acgcgctgca
    38641 acagtggcat cacttgtttg aagctgaagg gacaaaacgc tcaccgcaag cacagcagca
    38701 tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa catgaaaact ggaaatatac
    38761 gccgctggaa gggctgacca atagccagtt tgtcagcatt gcgggagaga tatccccaca
    38821 gcagcgagat gccttagcgt taacgttaga cgctgtgcgg ctggtatttg tcgatggacg
    38881 ttacgtgtca gcactgagcg atgcgactga aggcagcggg tatgaagtga gcattaacga
    38941 cgaccgtcag ggggtacccg acgctattca ggcggaagtg tttctgcatt tgacggaaag
    39001 cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt caacggccgg caaagccatt
    39061 gctgttaatg catattaccc agggcgtggc aggtgaagag gtcaacactg cccattaccg
    39121 acatcatctg gatctggcgg aaggtgccga agcaacggtg atcgaacatt ttgtcagcct
    39181 taatgatgct cgtcacttca ccggcgcacg gttcactatc aatgtcgcag cgaacgccca
    39241 cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt caccactttg cccataacga
    39301 tttgttgctg gctgacgatg ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt
    39361 gttacgacac aacaccagta cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag
    39421 cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt acctggctgg aacacaataa
    39481 aggtttttgt aacagccgac agttgcacaa aactatcgtc agcgacaaag gccgcgcggt
    39541 atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa acggatggtc agatgaccaa
    39601 caataatctg ctgatgggca aactggcaga agtggatacg aaaccgcagc tggaaatcta
    39661 tgcagatgat gtgaaatgca gccacggcgc gacggtgggg cgtattgatg atgaacagat
    39721 gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc cagcagatga tcatttacgc
    39781 ctttgctgcc gaactgacgg aagcactgcg tgatgagggg cttaaacagc aggtgctggc
    39841 ccgaatcggt caacggctgc caggaggtgc aagatgactt tttccgtcga caaagtgcgg
    39901 gccgactttc cggtgctttc tcgtgaggta aacggtttgc cgctggctta tctcgacagc
    39961 gccgccagtg cgcagaaacc gagccaggtg attgacgccg aggccgagtt ttatcgtcat
    40021 ggctacgcgg cggtgcatcg cggtattcat accttaagcg cccaagcgac cgagaaaatg
    40081 gagaacgtgc gcaagcgggc atcgctgttt attaatgccc gttcggcgga agagctggtg
    40141 ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca atagctgggg taacagcaat
    40201 gtgcgggcgg gcgataacat catcatcagt cagatggagc accacgctaa cattgttccc
    40261 tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg tgatcccgct caatcccgac
    40321 ggtacgttgc aactggagac gctgcctacg ctgtttgatg agaaaactcg cctgctggca
    40381 attactcatg tctccaacgt gcttggcaca gaaaatccac tggcggaaat gatcacgctt
    40441 gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc aggcggtgat gcatcatccg
    40501 gtggatgttc aggcgctgga ttgcgacttt tacgtgttct ccgggcataa actgtatggc
    40561 cccaccggaa ttggcattct ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg
    40621 gaagggggcg gttctatgat cgccaccgtc agcctgagtg aaggcacaac ctggaccaaa
    40681 gcaccttggc ggtttgaagc cggtacaccc aataccgggg gcatcattgg tcttggcgcg
    40741 gcgctggagt atgtttcggc gctggggctt aataacatag ccgagtatga acagaatctg
    40801 atgcactacg cgctatcaca gctggaatct gtaccggatc tcactctcta tggtccgcag
    40861 aacaggcttg gcgttattgc ttttaatctt ggtaaacacc acgcctatga tgttggcagt
    40921 tttctcgata actacggcat tgctgtgcgt accggacatc actgcgctat gccattaatg
    40981 gcctattaca acgtccctgc tatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa
    41041 gaagtggatc gtctggtgac cggactgcaa cgtattcacc gtctgctggg ataacaggga
    41101 ggcactatgg ctttattgcc ggataaagaa aaattgctgc gtaatttttt acgctgcgcc
    41161 aattgggaag agaaatatct ctacattatt gagctgggcc agcgtctgcc agaattacgc
    41221 gacgaagaca gaagcccaca aaatagcatt cagggctgcc agagtcaggt gtggattgtc
    41281 atgcgccaga atgcgcaggg aattattgaa ttgcagggcg acagcgatgc ggcgattgtg
    41341 aaagggctta ttgcggtcgt ctttattctc tacgatcaga tgacgccgca ggatattgtc
    41401 aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca cccaacatct caccccatct
    41461 cgttcacaag gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc
    41521 taaactaaag ggacagcttt catccggttg gctcacattg ccggatggtc aatgtttaca
    41581 ctgacctgca caggagtgtt tcggtttcag gtgaacataa gcgccatccg acgtttcagc
    41641 gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc ttacactcac
    41701 gcttatcggc gcttttagcg ccatccaggc tgcctgggcg gttgattatc cgctaccacc
    41761 aaccggaagc cgactggttg ggcaaaatca aacgtatacg gtgcaggaag gggataaaaa
    41821 ccttcaggcc atcgcccgac gttttgatac tgcggcaatg ttgatccttg aagccaataa
    41881 cactatcgcc ccggtgccaa aacccggtac gacgataact attccttcgc aactgttatt
    41941 acctgatgcg ccgcgtcagg ggattatcgt taaccttgca gagttgcgcc tttattatta
    42001 tccgccggga gaaaatatcg tgcaggttta tccgataggt attggcttgc aggggctgga
    42061 aacgccggtg atggaaacgc gtgtggggca gaaaatccct aacccaacct ggacgcctac
    42121 tgcaggcatt cgtcaacgtt cgctggagcg tggtattaaa ttaccgccag tcgttcctgc
    42181 cggaccaaat aacccgctag gacgttacgc actgcgcctc gcgcatggta atggcgaata
    42241 cctcattcat ggcaccagtg cgccggacag cgtcggtttg cgcgtcagtt cagggtgtat
    42301 tcgcatgaat gcaccggata ttaaagcctt gttctccagc gtgcggacgg gaacgccggt
    42361 gaaagtgatc aacgaacctg tgaaatattc cgtggagcct aacgggatgc gttatgttga
    42421 agtacatcga ccactatcgg cagaagaaca gcagaacgtt cagacaatgc catacacact
    42481 gccagcaggc tttacgcaat ttaaagacaa taaggctgta gatcagaagt tagtcgataa
    42541 agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc
    42601 cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt
    42661 acgcgcgacg cagtagcggt aaacggcaga caaaaaaaat ggcgcacaat gtgcgccatt
    42721 tttcacttca caggtactat tacttgcggt atttagtagc catgttgtcc agacgctggt
    42781 tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt
    42841 tgctcagctg gtcaacttta gcgttcagag tctgaacgtc agaagacagc tgatcgattt
    42901 tagcgttgct ggagcaacct gccagcagag tagaacccag gattaccgcg cccagtacca
    42961 gtttagtagc tttcattatt aataccctct agattgagtt aatctccatg tagcgttaca
    43021 agtattacac aaagtttttt atgttgagaa tatttttttg atgggaaggc acttattttt
    43081 gatcgttcgc tcaaagaagc atcgaacgct gggttttaga taaaaaaatt gagagaaaac
    43141 aggatgcttc catcggattc atcttagata aagtgagatt atatagtgaa atccgatttg
    43201 attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat
    43261 atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg
    43321 ccgctcggta ccagtgcacc agaaaccata actacaacgt cacctttgtg tgccagaccg
    43381 ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta
    43441 accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg
    43501 gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg
    43561 ccctgagtag caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca
    43621 cggcataccg cttcggtaat gcgcagttta cggttgtcat tgttgaactc gagacggctg
    43681 ttcatcacgc ggtcggtacg ttcgcaaatg gtcgccatga tagaaaccgc ttccagcggg
    43741 tatttacctt ttgcggattc accagacagc atcactgcat cagtaccgtc gaggatggcg
    43801 tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc
    43861 atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc
    43921 tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg
    43981 atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt
    44041 ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata
    44101 acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttg ttcgcaacca
    44161 aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc
    44221 aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg
    44281 ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg
    44341 ccaactgaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg
    44401 ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacgtcgtta
    44461 ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg
    44521 gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca
    44581 tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct
    44641 aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg
    44701 acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg
    44761 gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga
    44821 aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag
    44881 tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat
    44941 acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt
    45001 agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg
    45061 ctgtttgcgc aacgtctggt gccaaagttg tcagtgtgag gataagtga
//
LOCUS       DAZFDP010000022        44721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFDP010000022 DAZFDP010000000
VERSION     DAZFDP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(364..906)
                     /gene="ycfP"
                     /locus_tag="ACHJ1G_001324"
     CDS             complement(364..906)
                     /gene="ycfP"
                     /locus_tag="ACHJ1G_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4763211.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(929..1954)
                     /gene="nagZ"
                     /locus_tag="ACHJ1G_001325"
     CDS             complement(929..1954)
                     /gene="nagZ"
                     /locus_tag="ACHJ1G_001325"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4763212.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1965..2789)
                     /gene="thiK"
                     /locus_tag="ACHJ1G_001326"
     CDS             complement(1965..2789)
                     /gene="thiK"
                     /locus_tag="ACHJ1G_001326"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4763213.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2770..3411)
                     /gene="lpoB"
                     /locus_tag="ACHJ1G_001327"
     CDS             complement(2770..3411)
                     /gene="lpoB"
                     /locus_tag="ACHJ1G_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4763214.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3425..3802)
                     /locus_tag="ACHJ1G_001328"
     CDS             complement(3425..3802)
                     /locus_tag="ACHJ1G_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4763215.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3805..4164)
                     /gene="hinT"
                     /locus_tag="ACHJ1G_001329"
     CDS             complement(3805..4164)
                     /gene="hinT"
                     /locus_tag="ACHJ1G_001329"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4763216.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4498..6687
                     /gene="fhuE"
                     /locus_tag="ACHJ1G_001330"
     CDS             4498..6687
                     /gene="fhuE"
                     /locus_tag="ACHJ1G_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4763217.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6747..8180)
                     /gene="ptsG"
                     /locus_tag="ACHJ1G_001331"
     CDS             complement(6747..8180)
                     /gene="ptsG"
                     /locus_tag="ACHJ1G_001331"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4763218.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8475..9272)
                     /locus_tag="ACHJ1G_001332"
     CDS             complement(8475..9272)
                     /locus_tag="ACHJ1G_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4763219.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9283..10287)
                     /gene="holB"
                     /locus_tag="ACHJ1G_001333"
     CDS             complement(9283..10287)
                     /gene="holB"
                     /locus_tag="ACHJ1G_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4763220.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10284..10925)
                     /gene="tmk"
                     /locus_tag="ACHJ1G_001334"
     CDS             complement(10284..10925)
                     /gene="tmk"
                     /locus_tag="ACHJ1G_001334"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4763221.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10915..11937)
                     /gene="yceG"
                     /locus_tag="ACHJ1G_001335"
     CDS             complement(10915..11937)
                     /gene="yceG"
                     /locus_tag="ACHJ1G_001335"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4763222.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11940..12749)
                     /gene="pabC"
                     /locus_tag="ACHJ1G_001336"
     CDS             complement(11940..12749)
                     /gene="pabC"
                     /locus_tag="ACHJ1G_001336"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4763223.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12869..14110)
                     /gene="fabF"
                     /locus_tag="ACHJ1G_001337"
     CDS             complement(12869..14110)
                     /gene="fabF"
                     /locus_tag="ACHJ1G_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4763224.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14198..14434)
                     /gene="acpP"
                     /locus_tag="ACHJ1G_001338"
     CDS             complement(14198..14434)
                     /gene="acpP"
                     /locus_tag="ACHJ1G_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4763225.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14645..15379)
                     /gene="fabG"
                     /locus_tag="ACHJ1G_001339"
     CDS             complement(14645..15379)
                     /gene="fabG"
                     /locus_tag="ACHJ1G_001339"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4763226.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15392..16321)
                     /gene="fabD"
                     /locus_tag="ACHJ1G_001340"
     CDS             complement(15392..16321)
                     /gene="fabD"
                     /locus_tag="ACHJ1G_001340"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4763227.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16337..17290)
                     /gene="fabH"
                     /locus_tag="ACHJ1G_001341"
     CDS             complement(16337..17290)
                     /gene="fabH"
                     /locus_tag="ACHJ1G_001341"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4763228.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17358..18428)
                     /gene="plsX"
                     /locus_tag="ACHJ1G_001342"
     CDS             complement(17358..18428)
                     /gene="plsX"
                     /locus_tag="ACHJ1G_001342"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4763229.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18509..18682)
                     /gene="rpmF"
                     /locus_tag="ACHJ1G_001343"
     CDS             complement(18509..18682)
                     /gene="rpmF"
                     /locus_tag="ACHJ1G_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4763230.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18734..19255)
                     /gene="yceD"
                     /locus_tag="ACHJ1G_001344"
     CDS             complement(18734..19255)
                     /gene="yceD"
                     /locus_tag="ACHJ1G_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4763231.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19454..20038
                     /gene="yceF"
                     /locus_tag="ACHJ1G_001345"
     CDS             19454..20038
                     /gene="yceF"
                     /locus_tag="ACHJ1G_001345"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4763232.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20150..21109)
                     /gene="rluC"
                     /locus_tag="ACHJ1G_001346"
     CDS             complement(20150..21109)
                     /gene="rluC"
                     /locus_tag="ACHJ1G_001346"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4763233.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21682..24867
                     /gene="rne"
                     /locus_tag="ACHJ1G_001347"
     CDS             21682..24867
                     /gene="rne"
                     /locus_tag="ACHJ1G_001347"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4763234.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25063..26016)
                     /gene="flgL"
                     /locus_tag="ACHJ1G_001348"
     CDS             complement(25063..26016)
                     /gene="flgL"
                     /locus_tag="ACHJ1G_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4763235.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26028..27671)
                     /gene="flgK"
                     /locus_tag="ACHJ1G_001349"
     CDS             complement(26028..27671)
                     /gene="flgK"
                     /locus_tag="ACHJ1G_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4763236.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27737..28678)
                     /gene="flgJ"
                     /locus_tag="ACHJ1G_001350"
     CDS             complement(27737..28678)
                     /gene="flgJ"
                     /locus_tag="ACHJ1G_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4763237.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28678..29775)
                     /gene="flgI"
                     /locus_tag="ACHJ1G_001351"
     CDS             complement(28678..29775)
                     /gene="flgI"
                     /locus_tag="ACHJ1G_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4763238.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29787..30485)
                     /gene="flgH"
                     /locus_tag="ACHJ1G_001352"
     CDS             complement(29787..30485)
                     /gene="flgH"
                     /locus_tag="ACHJ1G_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4763239.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30538..31320)
                     /gene="flgG"
                     /locus_tag="ACHJ1G_001353"
     CDS             complement(30538..31320)
                     /gene="flgG"
                     /locus_tag="ACHJ1G_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4763240.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31458..32213)
                     /gene="flgF"
                     /locus_tag="ACHJ1G_001354"
     CDS             complement(31458..32213)
                     /gene="flgF"
                     /locus_tag="ACHJ1G_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4763241.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32233..33438)
                     /gene="flgE"
                     /locus_tag="ACHJ1G_001355"
     CDS             complement(32233..33438)
                     /gene="flgE"
                     /locus_tag="ACHJ1G_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4763242.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33463..34158)
                     /gene="flgD"
                     /locus_tag="ACHJ1G_001356"
     CDS             complement(33463..34158)
                     /gene="flgD"
                     /locus_tag="ACHJ1G_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4763243.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34170..34574)
                     /gene="flgC"
                     /locus_tag="ACHJ1G_001357"
     CDS             complement(34170..34574)
                     /gene="flgC"
                     /locus_tag="ACHJ1G_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4763244.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34578..34994)
                     /gene="flgB"
                     /locus_tag="ACHJ1G_001358"
     CDS             complement(34578..34994)
                     /gene="flgB"
                     /locus_tag="ACHJ1G_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4763245.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35149..35808
                     /gene="flgA"
                     /locus_tag="ACHJ1G_001359"
     CDS             35149..35808
                     /gene="flgA"
                     /locus_tag="ACHJ1G_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4763246.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35884..36177
                     /gene="flgM"
                     /locus_tag="ACHJ1G_001360"
     CDS             35884..36177
                     /gene="flgM"
                     /locus_tag="ACHJ1G_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4763247.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36182..36598
                     /gene="flgN"
                     /locus_tag="ACHJ1G_001361"
     CDS             36182..36598
                     /gene="flgN"
                     /locus_tag="ACHJ1G_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4763248.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36638..38173)
                     /gene="murJ"
                     /locus_tag="ACHJ1G_001362"
     CDS             complement(36638..38173)
                     /gene="murJ"
                     /locus_tag="ACHJ1G_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4763249.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38283..39205)
                     /locus_tag="ACHJ1G_001363"
                     /pseudo
     CDS             complement(38283..39205)
                     /locus_tag="ACHJ1G_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(39207..39854)
                     /locus_tag="ACHJ1G_001364"
     CDS             complement(39207..39854)
                     /locus_tag="ACHJ1G_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4763250.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39865..40449)
                     /gene="rimJ"
                     /locus_tag="ACHJ1G_001365"
     CDS             complement(39865..40449)
                     /gene="rimJ"
                     /locus_tag="ACHJ1G_001365"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4763251.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40685..41893
                     /gene="mdtH"
                     /locus_tag="ACHJ1G_001366"
     CDS             40685..41893
                     /gene="mdtH"
                     /locus_tag="ACHJ1G_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4763252.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41957..42604
                     /gene="grxB"
                     /locus_tag="ACHJ1G_001367"
     CDS             41957..42604
                     /gene="grxB"
                     /locus_tag="ACHJ1G_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4763253.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42738..43298
                     /locus_tag="ACHJ1G_001368"
     CDS             42738..43298
                     /locus_tag="ACHJ1G_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4763254.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43404..44450
                     /gene="pyrC"
                     /locus_tag="ACHJ1G_001369"
     CDS             43404..44450
                     /gene="pyrC"
                     /locus_tag="ACHJ1G_001369"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4763255.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44524..>44721
                     /gene="dinI"
                     /locus_tag="ACHJ1G_001370"
     CDS             44524..>44721
                     /gene="dinI"
                     /locus_tag="ACHJ1G_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4763256.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEI"
BASE COUNT        10814 a        12107 c        11137 g        10663 t
ORIGIN      
        1 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
       61 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
      121 ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa aacagagttt attgtttatt
      181 atttgagcaa gtccggaagg gcgatatagg gtgaagcgta tatcgagggc gtttggtcgt
      241 cctgataaga ggcgcatcag gcattgatgc tcgaatgtcg gatgcggcgt gaacgcctta
      301 tccgacccac agaatccgga gcgcatcagg taccggcaag gttacctgat gcgacgaggg
      361 catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa
      421 tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcagct cttcagatgt
      481 ccgctggctg ttaagcgctt catcattacg cgacaaaatc accaggcaac gatcgcgatt
      541 cttctcacgg aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc
      601 aatcttgcct tccatgttct cataagggaa caaattaggg ttgaagatca cctggcggat
      661 atcgcaaaga aaaccaatcc gttccgccca gtatccgccc agaccaacgc cgcaaatcag
      721 cggacgctcg tcgacgttca gttgcaacat tttgtccact tctttgagca gatgctgcat
      781 atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt caataaactg
      841 caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt gtaaatagat
      901 aatcatcgct tcctcacata agccagggtt agtgacctgc tttctcttcc tgccagcgtt
      961 catgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc atcagttcct
     1021 gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca
     1081 gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac
     1141 ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg gcaccttcca
     1201 tcgataaatc gtcagagaaa atcacgccgt caaaacccag ttcctgacgc aaaacggttt
     1261 tcagccagta gggagaaccg ctcgccggac gcggatcaac atcactgtag atcacatgcg
     1321 caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt
     1381 tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt gagtctgccg
     1441 ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct tcatgcatac
     1501 catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga taagaacgct
     1561 cgccaatcgc cgcgctgata tgcccgacat ccagcactgg cgcaaagctg atatcaatat
     1621 ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt ttgccgccct
     1681 cttccattcc tgacagcgca gcgaatgatt gcgccgctgg caagcgggta aaaccttcac
     1741 gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga ttgcgcgaag
     1801 ctgcgcggat ctggcgcacc agttcacgca actgggcagg atcatgatag ttacgcgtaa
     1861 agagaatcag ccctcccacc agcggatgcg caagtatttc acgctcttcc gcgtccagtt
     1921 cgtaaccttc gacatccaac attactggac ccacactgct ctccttattg ttttattaat
     1981 agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag
     2041 cggtactcaa accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga
     2101 cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccgg
     2161 tgctgttcag tattttccac ccacactgcc gccagttcca gcgcgatatc accatctccg
     2221 gcatactccc agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg
     2281 acatccatat gcaatggact taagcgtaaa ggccgtggtt cccgcgcttt gcgcagacgt
     2341 tttaacattc gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt
     2401 tccagtaacg gcaacagcgt tattcgccag ccaaaacgtg gttgttgatg tagataatac
     2461 agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttacctcgcc gggcagatag
     2521 tcgactacca tccagtcacg gagatataaa tgcggcttcg gtgcaatgct tgcgggtagt
     2581 tgtgataaag cccgatactg gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc
     2641 tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa aactcccgcc actaagccca
     2701 ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc
     2761 atgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg
     2821 caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg cgctggagta
     2881 cagcacgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac gggtacctaa
     2941 actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagctgct gggcggaaac
     3001 cagggtaaat ttcccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc
     3061 attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt
     3121 caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata
     3181 atggcgaata tgcggcgcag gcggtgccgt ttgatcttcg tgctcaattg ggcctggctg
     3241 ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct cggctggttg
     3301 ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc
     3361 gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca
     3421 ccccttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     3481 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     3541 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     3601 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     3661 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     3721 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacgcc agagacacca
     3781 gcccaaagca tccttttctc atcgttacag acctttatgt gccagcattg gtcccagcgg
     3841 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     3901 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     3961 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     4021 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     4081 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     4141 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     4201 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     4261 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     4321 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgtcaaata
     4381 tggatgcgta tatttctcat ttgcatttac atacaaaatt attcgcacac taaatataac
     4441 cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag atttcaaatg
     4501 ctttcaacac aatttaacag ggataatcaa tatcaagcca gcaccaaacc atcactactt
     4561 gccggttgca tagcattggc actattacct tctgccgctt ttgccgctcc agccactgaa
     4621 gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa tgattacagc
     4681 gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat tcctcagtcg
     4741 gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg
     4801 atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat
     4861 tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg
     4921 cgctggaatc tgggcgacgc actttctgat atggcactct ttgaacgcgt agaagtagtg
     4981 cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt
     5041 cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg
     5101 aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc
     5161 gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta caacagtgaa
     5221 aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac gctttcagcc
     5281 ggttacgaat atcagcacat tgatgttaat agcccaacct ggggcggttt accgcgctgg
     5341 aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc tgactgggcg
     5401 tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc
     5461 tggcaagcga cgctgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat
     5521 gtcgatgcct atgtaaacaa agcggatggt atgctgattg ggccatacag taattatgga
     5581 cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg
     5641 ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt
     5701 ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa catcttcccg
     5761 gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga ctggtcacca
     5821 cagagcctgg cgcaggacga taccacacat attaaatcgt tatatgctgc cactcgtgtc
     5881 acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg gcgggttgat
     5941 acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct ggtgtttgac
     6001 atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc gcaaaatgat
     6061 cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga gctgggtctg
     6121 aaatcggact ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag
     6181 gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat
     6241 aaagcagtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg cgcaattacc
     6301 gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa cgaaggaaac
     6361 gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct
     6421 gtcatgccag agttgacagt cggcggtggt gttaactggc aaaatcgcgt gtataccgac
     6481 accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct ggtggatctc
     6541 ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc
     6601 gacaaaacct acgataccaa cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc
     6661 agcattaccg gcacgtatca attctgaata gcacctgtaa aaaaggcagc catctggctg
     6721 ccttagtctc cccaacgtct tacagattag tggttacgga tgtactcatc catctcggtt
     6781 ttcaggttgt cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg
     6841 cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg
     6901 acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca
     6961 gcaaccagag ccggtgccat ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct
     7021 tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa
     7081 acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac
     7141 agaacgatga agtcgatcag accgtgcgag aacgacgtac cgtcacgcat ccccagaaga
     7201 atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca
     7261 acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc
     7321 gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata
     7381 gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc
     7441 gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg
     7501 ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac
     7561 ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa
     7621 gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat gaaggacagc
     7681 acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg tttaccggca
     7741 aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg
     7801 atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt agaggcgatt
     7861 tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg
     7921 ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa gccgagggcg
     7981 acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata
     8041 acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata
     8101 cctgcgatag gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta
     8161 gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt
     8221 ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt
     8281 tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac gtttcaactt
     8341 cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg
     8401 ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta attacgagct
     8461 ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg aaacagacgg
     8521 gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc
     8581 atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtacc
     8641 ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac ataacgcgca
     8701 gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat
     8761 ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca
     8821 ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg ggcgtcacgg
     8881 gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac
     8941 tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt ttcacccagc
     9001 gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc
     9061 tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc taccagatcc
     9121 cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt
     9181 gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca
     9241 tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg
     9301 tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag
     9361 ctcgcggttg atgcctgtaa cagacattaa ctgttcacga atgtggcaaa catcccccag
     9421 tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca ggcccggcac
     9481 atcaacattg gtcacctgcg cagcaccatg atggcgtttt agcgcatcca tcagcaacgt
     9541 tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcta gcagcgaata
     9601 ccagtcgccc gatggcacgc tatatgccaa cgcctgacac aatgtttcac gagcctgcca
     9661 gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc
     9721 ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg
     9781 ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg ccagtaaacg
     9841 ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt caagcgtttt
     9901 cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc agacaacttt
     9961 cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat
    10021 gcccagcata tttttccctt tttcgggagc cagggtgtag taatcgggat gcgtgccagc
    10081 ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca
    10141 gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg
    10201 aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc
    10261 aggtcgtaac catggatacc atctcatgcg tccaactcct taacccagtg ggtcacggta
    10321 gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatactt
    10381 ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac
    10441 tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg
    10501 gtaacatcga gatagagcgt taagtcgggg cgaaaatccc cgagaacagc atcacgcagt
    10561 gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg agtggagaga
    10621 tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat gaccgtttct
    10681 accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgacttca
    10741 tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg
    10801 ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt ctcaaccacc
    10861 acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg
    10921 cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat ggctggcaag
    10981 attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt
    11041 tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg cacccggcgg
    11101 caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag
    11161 tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct gcaggcgcat
    11221 accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac tggcaacggc
    11281 ggtttctttt tcgataattg atgccatcgt caccagctga tttttatctt tataaggcag
    11341 accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg
    11401 cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca
    11461 accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc
    11521 gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag
    11581 acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt
    11641 cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga
    11701 aagatccggt tcgatacgca gcagccattg aaaaacccgt gggcgattga tgatcttatc
    11761 agcataaagc tgttcaccga gcgccagacg tccggtccct ggcttcaggg taaatatcgt
    11821 ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc
    11881 agcggcgata cccagtacca ccagcaataa caagataatc aataacactt ttttcatgac
    11941 taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag
    12001 acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca
    12061 tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt
    12121 gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc
    12181 ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc
    12241 cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcgg
    12301 atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgagg attgcgcccc
    12361 agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc gtaatgtgca
    12421 ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct
    12481 cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg ttctgctgcc
    12541 agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat caaccgctga
    12601 caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact
    12661 ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat
    12721 tcctgcttat aaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg
    12781 gcagcatgtt cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg
    12841 accttttata tgggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag
    12901 tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc tgacgtgctt
    12961 catgcggtac gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg
    13021 gaacagcctg atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac
    13081 ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac
    13141 cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt
    13201 gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca
    13261 ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc
    13321 tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt
    13381 actcttcaag taccagcata ccggcaccat cgcccagtac gaagccatca cgctctttat
    13441 cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc
    13501 caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt
    13561 cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg ccggaagtac
    13621 aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg
    13681 ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt
    13741 tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg
    13801 cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct
    13861 gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg
    13921 agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc
    13981 tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg
    14041 tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac
    14101 gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa
    14161 aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt
    14221 aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat
    14281 caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt
    14341 cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga
    14401 taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg
    14461 ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca
    14521 ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt
    14581 gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc
    14641 gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg
    14701 cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac
    14761 ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt
    14821 ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc
    14881 gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac
    14941 cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt
    15001 tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga
    15061 taatttcgtt ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg
    15121 cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt
    15181 cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca cctaaataat
    15241 cgctgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg
    15301 ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga
    15361 tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg
    15421 aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa
    15481 gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa
    15541 cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca cggatggcat
    15601 caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt ggtgcgttaa
    15661 aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt
    15721 gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct ttacaggcag
    15781 cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa
    15841 agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc gcaatagacg
    15901 catcatccag accgatgatt gccgccatag cgccagtgcc ttccggtacg gcttcttgca
    15961 tgaacttacc gcgcatctca accagacgca ccgcatcagc gaaattaatc acgccagcgc
    16021 aaaccagggc ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc
    16081 cctgctgctg ccatacgcga tacagcgcaa cagatgcaat caacagcgct ggctgggttt
    16141 gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc
    16201 ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat
    16261 cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca
    16321 tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa
    16381 aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca
    16441 gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat
    16501 tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat
    16561 gcggaaccag ccagtccagt tgagaacggt caagattatt tgccgccagc gtctcatcaa
    16621 cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat
    16681 gaattgaatt ctctggattc acacggtcag cattaggcag cgtcagcaac tcaccatagc
    16741 taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca gccagcaccg
    16801 cagcgcccgc gccatcgcca aaaataataa tagtcccacg atcggttgga tcgcaggtgc
    16861 gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt
    16921 gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg
    16981 ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg
    17041 tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct
    17101 caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa
    17161 tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt
    17221 tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct
    17281 tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt
    17341 tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa
    17401 ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc
    17461 gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt
    17521 tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg
    17581 tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac
    17641 gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct
    17701 tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg
    17761 ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta
    17821 agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc accaatattg
    17881 agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac tgagcccata
    17941 atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa atcgaggacc
    18001 accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg ctcaatcccc
    18061 tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg
    18121 acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat tgaactccca
    18181 cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga
    18241 ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg
    18301 tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct
    18361 gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga
    18421 cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct
    18481 cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt
    18541 aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca
    18601 ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg
    18661 gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa
    18721 cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt
    18781 gcgcttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag
    18841 aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca
    18901 gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag
    18961 cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg
    19021 gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg
    19081 cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga
    19141 ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg taatcaaggc
    19201 gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc
    19261 gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt
    19321 tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga
    19381 acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct
    19441 gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc gtcgcgctct
    19501 tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg aaaccccacg
    19561 cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct
    19621 ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg
    19681 cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag
    19741 cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga atgggcatct
    19801 acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga
    19861 taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt
    19921 tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg ttggtttacc
    19981 gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga tgggatagtt
    20041 gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc tgatgcggcg
    20101 taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat tagcgcgcgt
    20161 tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg atacgcatca
    20221 cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac agacgattta
    20281 atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag cgatcgtcaa
    20341 aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta cgtccggtta
    20401 ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta aagcgtgttt
    20461 ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc agaatatttt
    20521 tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca
    20581 ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc aacgccgagc
    20641 gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga accagttcaa
    20701 ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag cttaaaccac
    20761 tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga tcatcttcat
    20821 ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt
    20881 cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg agtttatatt
    20941 caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata cggtaaatca
    21001 tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt
    21061 cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg gtgggcgatt
    21121 ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta gttttacacc
    21181 ttattacttc actacgtgat cactttattg atggttatta aaccaatcac cagcaagaag
    21241 tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct
    21301 tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg
    21361 aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt
    21421 tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag
    21481 agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa
    21541 ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca
    21601 cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag tcgtcaatgt
    21661 aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag
    21721 ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg
    21781 cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg
    21841 gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt
    21901 gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg
    21961 ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc
    22021 gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat gccgaacaac
    22081 ccgcgcgcgg gtggcatttc tcgccgtatc gaaggcgacg accgtaccga attaaaagaa
    22141 gcactggcga gccttgaact gccggaaggc atggggctta tcgtgcgcac cgctggcgtc
    22201 ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gtctgaaaca ctgggaagcc
    22261 atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta
    22321 atcgttcgcg cattccgcga ttacttacgt caggacatcg gcgaaatcct tatcgataac
    22381 ccgaaagtgc tcgaactggc acgtcagcat atcgctgcat taggtcgccc ggatttcagc
    22441 agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg
    22501 cagatcgaat ccgccttcca gcgtgaagtc cgtctgccgt ccggtggttc cattgttatc
    22561 gacagcaccg aagcgttaac cgccatcgac atcaactccg cacgcgcgac ccgcggcggc
    22621 gatatcgaag aaactgcgtt taacaccaac ctcgaagctg ccgatgagat tgctcgtcag
    22681 ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgcctgta
    22741 cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgtgcgcgt
    22801 attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgtca gcgcctgagc
    22861 ccatcactgg gtgaatccag tcatcacgtt tgtccgcgtt gttctggtac tggcaccgtg
    22921 cgtgacaacg aatcgctgtc gctctctatt ctgcgtctga tcgaagaaga agcgctgaaa
    22981 gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta cctgctgaat
    23041 gaaaaacgtt ctgcggtaaa tgccattgaa actcgtcagg acggtgtgcg ctgtgtaatt
    23101 gtgccaaacg atcagatgga aaccccgcac taccacgtgc tgcgcgtgcg taaaggggaa
    23161 gaaaccccaa ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg
    23221 tctgaagaag agttcgctga acgtaagcgt ccggaacaac ctgcgctggc aacctttgcc
    23281 atgccggatg tgccgccagc gccaacgcca gctgaacctg ccgcgcctgt tgtagcccca
    23341 gcaccgaaag ctgcaccggc aacaccagca actcctgcac aacctgggct gttgagccgc
    23401 ttcttcggcg cactgaaagc gctgttcagc ggtagtgaag aaaccaaacc gaccgagcaa
    23461 ccagcaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag
    23521 aacaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac tgaaggcagc
    23581 gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcagac tgccgagacg
    23641 cgtgagagcc gtcagcaagc tgaggtaacg gaaaaagcac gtaccaccga cgagcagcaa
    23701 gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa
    23761 gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta
    23821 cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa
    23881 agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc tgccgaacca
    23941 attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct gccagtcgta
    24001 gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc
    24061 atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca gcgtcgtcgt
    24121 cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcgcg
    24181 tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat
    24241 gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat ggtgactgag
    24301 gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga agaaatggct
    24361 gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt tgaaacgacg
    24421 catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac cgagtctgat
    24481 gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc gcaggaagag
    24541 acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga acctgaagtt
    24601 gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga aacggtagcc
    24661 gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat gacgcgcgct
    24721 ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg ccctactttt
    24781 gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc ctctgccgct
    24841 cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc cctggtaact
    24901 gccagggctt ttttatttca tctttgaatc aacatattaa aattcatcaa atggcatcct
    24961 tgctaaccaa caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc
    25021 agacaaaaca tacccagttt catgatatgt tttaaagcga gcttatttgt tgagctggaa
    25081 gagcgacaat ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg
    25141 catgatgtaa gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg
    25201 cgtttgccct aaagcgcgat cgctacctaa tgaatccagc gactccagtt cgttcagctg
    25261 cgtgcctaat tccgcgcgaa ctgtaaggac attattcagc gagtttttca gtccacggtt
    25321 ggttttatcc aatgccgcag cggcgatttc tttatccgct tcgctatccg cgaccggcgt
    25381 tttcagggct gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc
    25441 gtctggttcc gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc
    25501 tatcaccatc gaacgcgaag catcgacctg ttgcctaata ctttctgctc caccgacgta
    25561 tttccctttc tcttcgctaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg
    25621 cccgttaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctggatatc
    25681 cgtagccagc gaggcccggt catcgtcact caaagtgcca ttgctggcgt agacaatttt
    25741 ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac
    25801 tttttgagtg gcgaaagtac gcgccagcgt gtactggctg ttttgcgcct gtgcctggga
    25861 gagaacaacc gcttgtgatg ctgcaatggg atcgtcagaa gggttaacga ctcgcttacc
    25921 tgtcgacatc tgctcgccgt acttcatcca ttccgcctga gaattggtga tgccacgcat
    25981 gttttgctgg tacatcatct gggtactgaa acgcatctta tctcccctta gcgaatgtta
    26041 atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac
    26101 tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc
    26161 tgctgattgg aaagctgcgt caccacatta ccttgcgtgg cgctactggt tttcaacgtc
    26221 gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca
    26281 ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt gcggttatcg
    26341 ctatcacccg catcttcttc gctcgccatc gctattttcg cttcgtcggt gattaataca
    26401 tccatgttga cgatagcgtc acttactggt ttcagcgtga agctgtcgtt aacagcaggc
    26461 gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc atccggcgtc
    26521 accgtaaaag tggtattact ggcaaggcgg gtgacctgcc actgattatt atcgaacgag
    26581 attttgtaat cagtcgccag taccgcggag gcatcagtta ccgtggcacc gatcgcaacg
    26641 tcacctgtgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagcaaa gaaatcttca
    26701 ccagcatcgc cgttggcatc aaatccggct ttgtgttggc tgttgaaagc ctcggcaaat
    26761 gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga acggaatgtc
    26821 agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc
    26881 gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg aactgccgcc
    26941 agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggccatcgt gatgttataa
    27001 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    27061 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    27121 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    27181 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    27241 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc
    27301 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    27361 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcagtcagg
    27421 ccgctacttt gcgtctgcgc cgcacgtaac tggttggtaa taaaagcatc atactcacgc
    27481 tgcacaccag aaacgtagac accattgcca acccagccgc cagcgcccaa cgtgctattg
    27541 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    27601 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc
    27661 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    27721 gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct
    27781 tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat
    27841 cggtagcata ccccgcgtcc tgtagggcct gcgccccctg ctccgcactc gcggcggtcg
    27901 tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca
    27961 gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    28021 cagtcgtggt gatttcagta actggccctt tccagttgcc agaggctttg acaccaaaca
    28081 ggttatagct cggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcca
    28141 gtgccgcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    28201 cgggcagcga gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt
    28261 tacgtggcac ggccttttgc accagctgcg aaagcgcctg attttgataa cgcaccacag
    28321 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    28381 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    28441 gggcaatctg ctggtcatac atactggtat acaggcgagt gtgctcgctg ctgaacaggc
    28501 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    28561 cctgacgggc caccggacgg atatttgccg ccggatcttc gcccgctttc gcctttagtt
    28621 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    28681 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca gtatggacat
    28741 cagatccatc ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc
    28801 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    28861 agtaaccaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    28921 attgactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    28981 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    29041 attaacctgc atattctgga tatctgcaag gaaacggacc tgggaactgt tgccacttgg
    29101 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    29161 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    29221 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    29281 gatccgtcca ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc
    29341 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    29401 cggcgtcatc aacaacgtac ctccacgcaa gcttttggca tttcccatgg aagaaaccac
    29461 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc
    29521 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    29581 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc
    29641 cagccccacc accagaccat agccaatcag tgagttttgc cttaccccct gaacactggt
    29701 gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga
    29761 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    29821 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca
    29881 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    29941 ccagagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    30001 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    30061 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    30121 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    30181 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    30241 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    30301 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcggc
    30361 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    30421 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    30481 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttaagcctta
    30541 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    30601 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    30661 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    30721 tgcaccagag gattgcgttt cggtgtagag gttttcgcca atgctctcca gcccggtgtc
    30781 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    30841 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    30901 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    30961 gaaagagccg tcacgggtat aggctgatga accatctggc aacatcacct ggaaaaagcc
    31021 ctgcccttta atcgcgacat ctttgctgtt gttggtctgc gacaggtttc cctggctgtg
    31081 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    31141 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    31201 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    31261 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    31321 aaaatgtcct gtagtggatt agtgacaagc agagcagaag cacgcgacag tgtgaatttc
    31381 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    31441 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    31501 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    31561 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    31621 gttggatctg cctgcagtat cggcccacgc gtggcctggc tttctgcgct taaacgaaaa
    31681 atgccgtcgt caccgcgttg cacttcgctg cccgtggctt tcaccagttt cagacgcccc
    31741 actggcgcaa ccgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    31801 gcaatagtga tttccgcccc ttccggcaca gcaattggcc cagcctcgcc tatcaccgga
    31861 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    31921 ccttcgctgc cgtcagcgga ctgcacggcc agccagccat cctgctgcaa tgcaacgtcc
    31981 agcgggcgcg aggtgtaatc catcttgccg ggcgtcatgt ctgcacccgg tgttgacgcc
    32041 gttaccagcg tgcgcgtggg cagtgaaagc ccttccaccg gcactgcacg caaggcgttt
    32101 aactgcgcgc gaaaaccggg cgtagaggca ttggccagat tactggcggt taccgcctgt
    32161 tgattcagcg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    32221 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gggttttgat
    32281 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    32341 gagatcgacg ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    32401 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    32461 ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    32521 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    32581 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    32641 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    32701 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    32761 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    32821 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    32881 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    32941 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gatgccgtgg tggtagtttt
    33001 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    33061 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    33121 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    33181 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    33241 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    33301 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    33361 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    33421 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    33481 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    33541 gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    33601 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccgt cagtcaacgt
    33661 accgtcccag gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac
    33721 aaccgcacca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    33781 aacaccaaac ggcgtggtcg tggtcacagc tccttcttca ctgccggttc ccgcaagaac
    33841 agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    33901 gttatcaatc tgtccggaaa tagatccgag tgtggtattg agtttttcaa tcccgctgac
    33961 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    34021 ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt
    34081 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    34141 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    34201 atgcttttca ccgtgttgag cacttcaacg ttggcctgat agctgcgtga cgccgacatg
    34261 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatct
    34321 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    34381 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    34441 tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg
    34501 gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    34561 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    34621 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    34681 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    34741 agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga ggtcatcgtc
    34801 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    34861 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    34921 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    34981 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    35041 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatggt
    35101 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctgacaata
    35161 aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc gagcaatctc
    35221 acatcgcaat tgcacaactt ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt
    35281 gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt gctttcgatg
    35341 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    35401 cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcccatt
    35461 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gctggatacc
    35521 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccattag cctgcgcgat
    35581 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    35641 ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag
    35701 gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc gggacaggta
    35761 gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat
    35821 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    35881 accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa
    35941 accactgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc caccagcacc
    36001 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    36061 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    36121 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    36181 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    36241 ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    36301 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    36361 caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    36421 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    36481 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    36541 tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt cgatctgaat
    36601 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    36661 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    36721 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    36781 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    36841 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    36901 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    36961 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    37021 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    37081 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    37141 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggaccagaa agaatgccca
    37201 acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag tccatcaaac
    37261 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    37321 tgccaagcgc cacgcccagc acaccggacg gaaactccat taagcggtcg gcgtaataca
    37381 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    37441 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgttc
    37501 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    37561 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    37621 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    37681 ttggagcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    37741 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    37801 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    37861 cgagcatgcc agcgaccgtc acaaccgcca gcgcgagtgt cagcaggcca gaaacataag
    37921 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    37981 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    38041 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    38101 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    38161 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    38221 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    38281 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcacggcc
    38341 tgttcgcggc ggtttgcgga actgtctgat tttgcacaca ttcaatgaag tgccgcgcac
    38401 agccgacaaa tccacgttgt tcaagagtgc tctgccaacc aggaatcggt ttatgcacta
    38461 cgccctgccc gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt
    38521 cagtcacggc ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caggtggtga
    38581 tctgcaaagg accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag
    38641 tcagtagcgt accgccatcc agagaggctt tgccgcccga caaccacagc acggtatcca
    38701 ccacatgcag ataatcatcc agcaacgtga aataaagatc gtgtggcccg acactattgc
    38761 tacgatgttt atccattctt agcgaagctg cggtagcgag ttgcgttttt aactcaccgt
    38821 agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt cagtttttta cgcgccgcca
    38881 gttccaccag ccgttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat
    38941 gtacacccgc attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggaatgca
    39001 caaaaaccgc atcgcaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc
    39061 agctttcaca aattggcagg gctttcgcgc gcgtaggcga ccaggctcct tgtaacgtcc
    39121 agtcagacgc tgccgccagc accggtaacc acgctttttg cgcaatgcca cctaatccca
    39181 ctacgccgat acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga
    39241 cgctgtttca gttctgccac ttcgatttcc agggcttcga cgcgggcctg taaatcacca
    39301 tcaaccgcgt tcgacatatc cgtcaccgcc ggctgatctt caacctcacc actgaaaaga
    39361 tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca
    39421 tcttcgcgat ttgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca
    39481 tacattcgcg cggcgcggct gcgcagttca cctggcgtct gggcaccacg caataacaac
    39541 gtggtgatta acgccacttc cgctgcgctc agtttcagat cgccaaattc tgaattacaa
    39601 aaacgttgct catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga
    39661 cgtttgacca gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt
    39721 tcacggttcg ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc
    39781 tccggcgtgg tcacctgctt ttccagcaag caaccaatca cgcgagcttc aagggcagta
    39841 agttgatatt tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc
    39901 cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc
    39961 tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt
    40021 agccataatg cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc
    40081 ttcaaacatg agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc
    40141 atgaaaagag ccacgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc
    40201 cgggtcgaat aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc
    40261 cagcctggcc tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg
    40321 cttgaggaaa tggcgattct ctgcgtaata atccgcaaga cgccaggcat cacgatcatg
    40381 caccagacgc acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata
    40441 gccaaacatt ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta
    40501 tacctgcgca accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata
    40561 tttgataaaa actctctatc aaagcgacgt tttgataaaa aaatattgtc cccgaagtga
    40621 tgggaaaagt cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg
    40681 ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa
    40741 tatgctggtc gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga
    40801 tcaaatgggc tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca
    40861 gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat
    40921 tgttaccggt atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc
    40981 gtggctattg tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc
    41041 gcgttcggcg ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct
    41101 gttgatgatg caggacagtg ccggtgcggt cattggcgca ttgttgggga gctggctgtt
    41161 gcaatacgac tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tgtgtgcggc
    41221 gttcaatgcg tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga
    41281 aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg
    41341 ttactacatg ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc
    41401 tggcgcaccc tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt
    41461 gctctaccct atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc
    41521 tgggctgttg ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctgcaaca
    41581 acttttcacc ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga
    41641 aaccttaagt gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg
    41701 tctgggtctg gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct
    41761 gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg ctgggcatta ttggcatctt
    41821 cactttcctt gcgctgggtt ggcagtttag ccagaaacgc gccgcgcgtc gtttgcttga
    41881 acgcgacgcc tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc
    41941 caaatggagg agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc
    42001 ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga
    42061 cgcagaaaca cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga
    42121 cagccgctat atgccagaaa gcatggacat cgttcactat gtcgataaac tcgacggcaa
    42181 accgttactg accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg
    42241 ctacgcaaac aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac
    42301 tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta atttagccga
    42361 cctgctggcc cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga
    42421 caaactgatc gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct
    42481 atttccgcta ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc
    42541 tgattaccgc gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat
    42601 ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc
    42661 tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt
    42721 tcatgtcgag gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc
    42781 tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt
    42841 cgcttgcgaa acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc
    42901 atattgttct gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc
    42961 taaccggaga cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga
    43021 aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag
    43081 agatggaagt ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt
    43141 tgcttcccta tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc
    43201 tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag
    43261 tgaagccagg cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc
    43321 tttatttttc gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc
    43381 cttcagccgg agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc
    43441 cagacgactg gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata
    43501 ccagcgaaat ttatggacgg gctattgtaa tgcccaatct ggctccgccc gtgaccactg
    43561 ttgaggctgc cgtggcgtat cgccagcgta ttcttgatgc cgtacctgcc gggcacaatt
    43621 tcaccccatt gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg
    43681 gatttaacga aggcgtgttc accgctgcaa aactttatcc ggcaaacgca accactaact
    43741 ccagccacgg cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa
    43801 tcggtatgcc gctactggtg catggtgaag tgacacatgc agatatcgac atttttgatc
    43861 gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga
    43921 aagtcgtttt tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg
    43981 aacggctggc tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg
    44041 ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac
    44101 aggcattgcg tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg
    44161 cgccacatgc acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc
    44221 caaccgcgct gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg
    44281 aagcattctg ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca
    44341 tcgaactggt acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc
    44401 tggtgccatt cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc
    44461 ttcctgttgt cattaggtta ttttacctgt ataaataacc agtatattca acagggggct
    44521 attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac
    44581 gccctggctg gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac
    44641 gtatcggtac gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat
    44701 aaacagcgca ttagcgaaat t
//
LOCUS       DAZFDP010000023        41354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFDP010000023 DAZFDP010000000
VERSION     DAZFDP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..116)
                     /locus_tag="ACHJ1G_001371"
     CDS             complement(<1..116)
                     /locus_tag="ACHJ1G_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4763257.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQV"
     gene            complement(127..537)
                     /locus_tag="ACHJ1G_001372"
     CDS             complement(127..537)
                     /locus_tag="ACHJ1G_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4763258.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAALPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            complement(534..812)
                     /locus_tag="ACHJ1G_001373"
     CDS             complement(534..812)
                     /locus_tag="ACHJ1G_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763259.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(1101..2855)
                     /locus_tag="ACHJ1G_001374"
     CDS             complement(1101..2855)
                     /locus_tag="ACHJ1G_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO4763260.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLARERQNLSDGLVIELPVKQKAQLSQMADSERAQLLADRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            complement(2852..3151)
                     /locus_tag="ACHJ1G_001375"
     CDS             complement(2852..3151)
                     /locus_tag="ACHJ1G_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4763261.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSRKKARANIQEIKKMLNVFRGKK"
     gene            complement(3157..3390)
                     /locus_tag="ACHJ1G_001376"
     CDS             complement(3157..3390)
                     /locus_tag="ACHJ1G_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763262.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(3383..3616)
                     /locus_tag="ACHJ1G_001377"
     CDS             complement(3383..3616)
                     /locus_tag="ACHJ1G_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763263.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEAHYRRKFPEDVSHA"
     gene            complement(3609..3848)
                     /locus_tag="ACHJ1G_001378"
     CDS             complement(3609..3848)
                     /locus_tag="ACHJ1G_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763264.1"
                     /translation="MCNNTRPDAAAEAIITLMHALIDISVIADRAHKHDTSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGYA"
     gene            complement(3838..4044)
                     /locus_tag="ACHJ1G_001379"
     CDS             complement(3838..4044)
                     /locus_tag="ACHJ1G_001379"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4763265.1"
                     /translation="MRNHTTHPQGRDSHDLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQEVCHVQ"
     gene            4244..4423
                     /locus_tag="ACHJ1G_001380"
     CDS             4244..4423
                     /locus_tag="ACHJ1G_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4763266.1"
                     /translation="MATGTTNAKSQALKARVPHEIVNAMESVKESGESTSQFIIASMQ
                     GEIKRRQRRKAKESE"
     gene            complement(4426..4866)
                     /locus_tag="ACHJ1G_001381"
                     /pseudo
     CDS             complement(4426..4866)
                     /locus_tag="ACHJ1G_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4924..5112)
                     /locus_tag="ACHJ1G_001382"
     CDS             complement(4924..5112)
                     /locus_tag="ACHJ1G_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4763267.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(5487..6716)
                     /locus_tag="ACHJ1G_001383"
     CDS             complement(5487..6716)
                     /locus_tag="ACHJ1G_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4763268.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEAKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6704..6886
                     /locus_tag="ACHJ1G_001384"
     CDS             6704..6886
                     /locus_tag="ACHJ1G_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763269.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHJ1G_001385"
                     /pseudo
     CDS             6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHJ1G_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHJ1G_001386"
     CDS             complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHJ1G_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4763270.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHJ1G_001387"
     CDS             complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHJ1G_001387"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4763271.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHJ1G_001388"
     CDS             11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHJ1G_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4763272.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHJ1G_001389"
     CDS             12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHJ1G_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4763273.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            13827..14549
                     /gene="folM"
                     /locus_tag="ACHJ1G_001390"
     CDS             13827..14549
                     /gene="folM"
                     /locus_tag="ACHJ1G_001390"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4763274.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHJ1G_001391"
     CDS             complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHJ1G_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4763275.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            15010..15729
                     /gene="rstA"
                     /locus_tag="ACHJ1G_001392"
     CDS             15010..15729
                     /gene="rstA"
                     /locus_tag="ACHJ1G_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4763276.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            15733..17034
                     /gene="rstB"
                     /locus_tag="ACHJ1G_001393"
     CDS             15733..17034
                     /gene="rstB"
                     /locus_tag="ACHJ1G_001393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4763277.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            17110..18039
                     /gene="tus"
                     /locus_tag="ACHJ1G_001394"
     CDS             17110..18039
                     /gene="tus"
                     /locus_tag="ACHJ1G_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4763278.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHJ1G_001395"
     CDS             complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHJ1G_001395"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4763279.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHJ1G_001396"
     CDS             complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHJ1G_001396"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4763280.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21427..22602
                     /gene="manA"
                     /locus_tag="ACHJ1G_001397"
     CDS             21427..22602
                     /gene="manA"
                     /locus_tag="ACHJ1G_001397"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4763281.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHJ1G_001398"
     CDS             22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHJ1G_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4763282.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(24256..25521)
                     /gene="uidC"
                     /locus_tag="ACHJ1G_001399"
     CDS             complement(24256..25521)
                     /gene="uidC"
                     /locus_tag="ACHJ1G_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO4763283.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(25560..26933)
                     /gene="uidB"
                     /locus_tag="ACHJ1G_001400"
     CDS             complement(25560..26933)
                     /gene="uidB"
                     /locus_tag="ACHJ1G_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4763284.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(26930..28741)
                     /gene="uidA"
                     /locus_tag="ACHJ1G_001401"
     CDS             complement(26930..28741)
                     /gene="uidA"
                     /locus_tag="ACHJ1G_001401"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO4763285.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(29130..29720)
                     /gene="uidR"
                     /locus_tag="ACHJ1G_001402"
     CDS             complement(29130..29720)
                     /gene="uidR"
                     /locus_tag="ACHJ1G_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4763286.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(29941..30708)
                     /gene="hdhA"
                     /locus_tag="ACHJ1G_001403"
     CDS             complement(29941..30708)
                     /gene="hdhA"
                     /locus_tag="ACHJ1G_001403"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4763287.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(30820..31848)
                     /gene="malI"
                     /locus_tag="ACHJ1G_001404"
     CDS             complement(30820..31848)
                     /gene="malI"
                     /locus_tag="ACHJ1G_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4763288.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            32023..33615
                     /gene="malX"
                     /locus_tag="ACHJ1G_001405"
     CDS             32023..33615
                     /gene="malX"
                     /locus_tag="ACHJ1G_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4763289.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33625..34797
                     /gene="malY"
                     /locus_tag="ACHJ1G_001406"
     CDS             33625..34797
                     /gene="malY"
                     /locus_tag="ACHJ1G_001406"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4763290.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            34901..35902
                     /gene="add"
                     /locus_tag="ACHJ1G_001407"
     CDS             34901..35902
                     /gene="add"
                     /locus_tag="ACHJ1G_001407"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4763291.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(35937..36977)
                     /gene="ydgJ"
                     /locus_tag="ACHJ1G_001408"
     CDS             complement(35937..36977)
                     /gene="ydgJ"
                     /locus_tag="ACHJ1G_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4763292.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            37220..37345
                     /gene="blr"
                     /locus_tag="ACHJ1G_001409"
     CDS             37220..37345
                     /gene="blr"
                     /locus_tag="ACHJ1G_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4763293.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            37618..37833
                     /gene="ydgT"
                     /locus_tag="ACHJ1G_001410"
     CDS             37618..37833
                     /gene="ydgT"
                     /locus_tag="ACHJ1G_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4763294.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            37919..38359
                     /gene="ydgK"
                     /locus_tag="ACHJ1G_001411"
     CDS             37919..38359
                     /gene="ydgK"
                     /locus_tag="ACHJ1G_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4763295.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            38436..39017
                     /gene="rsxA"
                     /locus_tag="ACHJ1G_001412"
     CDS             38436..39017
                     /gene="rsxA"
                     /locus_tag="ACHJ1G_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4763296.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            39017..39595
                     /gene="rsxB"
                     /locus_tag="ACHJ1G_001413"
     CDS             39017..39595
                     /gene="rsxB"
                     /locus_tag="ACHJ1G_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4763297.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            39588..>41354
                     /gene="rsxC"
                     /locus_tag="ACHJ1G_001414"
     CDS             39588..>41354
                     /gene="rsxC"
                     /locus_tag="ACHJ1G_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4763298.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         9922 a        10797 c        10014 g        10621 t
ORIGIN      
        1 acctgatgcc gttccttgtc tgtctccacc agcagcatga cttcacccca tcgcgccgcc
       61 gccctccggt aaaagccttt cgcctcgagt tcttccgcta tgcggtcatg taccatcgcc
      121 accccctcag aacggaatac cgtcaccgta agggtcatcg cctcccgctg gtggctgatt
      181 accctgtgtg cctgtggttt tgcgtctgtt cccgccagga agtgccgcac gggcactgat
      241 tacgctgtct gcaataacct gataaccctg ccgcgtttcc ccattctgtc cggtccactg
      301 gctgacctgc atcgtgccgg atacgctggc aacgtcgcct ttttgatgtt tagccaggaa
      361 gtcggcctgc ttaccaaatg cgatgaccga tagccataac gtcgcctgcc catcctgcgc
      421 ctgactacat ggcagcgata ccgccatacg cgccagcgtc atcggtgtac ccttgctggt
      481 ctgttttacc tgcgggtcgt ccaccagccg cccgtaagct gctatctgtg ctgtcatgat
      541 tccacctctc cggttttaac gttgatggtt gttacctgtt ccgcttcggc aatctcccgc
      601 tctgtcagcg tggcaaaatt tgccgccgtc gtggtcatga atgcgcttat cagttcggga
      661 tgtgctttcg cgtatccttc ccccgcgtgg cggtctatcg ttctgattgc cacctttaaa
      721 gcgtgctctg tcatgtctaa cgcgcgatat ttcggttctg ttctgtctct gcgttttttg
      781 agtgatttat taaatttccc tgaagtgtgc atatttattt ttaccccctc gtttaaaaag
      841 ttttgagttg tgcctcccct tgtctacctt atctacctta gtggccctca taccagtaat
      901 ggcgcggctt tcagctgggt agagtgcttt tatccactat ctaccccgtg tctacctccc
      961 tgtctgattc aggtaaaatc aggtagagag ggtagatagt gggtagacag taaaaaaagg
     1021 ctatctacct aacttaatgc actgaattaa atgtattttt ctttactcag gtagacaggg
     1081 tagacagcaa ttacaaaaaa ttataaaaac gcgtcgcact cgtctgttgt tattgcgtta
     1141 gtctgcgtta ctcccttaac tttccgcgta atatattcat gtccgtaaac tttcgcggct
     1201 ggcttcatag ccttgccaaa gtcatttacg tttagcggtt tgctcctgcc tgcgtacgcc
     1261 ataaacgcca gatagacgcg gtaaaggctg tttctggtcg tgtacttcac tgaatcgcca
     1321 ccgccgccca tcatcaggcc gcgtgcttcc tccagaaaat tcaggaactg gcaaaactca
     1381 ataaccggat ccgtctgttg ctttattgcc agagcttcat caccgtcacg ctgttccagt
     1441 agtaaagccc gtgccttctc agggtcggta aagttcgcca gcaatcggcg gataatgaca
     1501 gggatttcag ccgcgatctt ttccggtagc tccctgtctt tttcggcctc actgacgata
     1561 ttgtcgaaac ggaaaatcac gcgacgacgt gccacacctc cggcccgttc ggtgaatatc
     1621 atcgggttat tgttggtcgc cagcaccacc gccctgatta cagccgtgaa acgcttttcg
     1681 tatttcgggt taatttccac ggggtcaccg cccgtgattt tcttgatgcc cgttccttcg
     1741 cctgtatatt tcggctggtc agccaggacg ataagacgac tcccgacaac ctgcgcacgt
     1801 ccaccagcat catcaagcga tgtcatttca gcgcttaccg tgttctgttt ccctgccaga
     1861 aggctggcta tgtgcgtgaa tgtactttta ccgctcccgc cgtctccggt ggcctcaata
     1921 aacatctgcc agtcgtaccg gttcgccata atcatgtaca gcgcggcaca tatacgcatc
     1981 atcttgcgcg ggtcttttcc ggctgcgtgc tcaagccatt tatgaaagtt tggcgcgtta
     2041 tcgcggatgt tctcccctgg tgctggtggc gtgtactcaa tgccgttgtg cgtggtgatc
     2101 cagttctccg gcgtgtgcgg ggaaaattcc cccgttttca ggtcaagcgc accattggcg
     2161 aacggcagca aatcgccgga cggctcgccc attggttcgg caataacttt taacgcttcc
     2221 acggcgttat tgattacgcg cttgctgaaa gtggccctgt gctctgaata gatcgccacc
     2281 atttcgcggc taagttccat tgtgctgacc ggacaccata ccccgccgcg ccatacgtga
     2341 acgatttcac tttcaggatg tacgcaaacg ccatcaaagc gatcggcaag cagctgcgcg
     2401 cgctcactgt ccgccatctg cgaaagttgc gccttttgct ttaccggaag ctcaatgacc
     2461 agaccatcag aaagattctg gcgctcacga gccagatatt cgcgccagtt ctccaccttc
     2521 tgaccgtgca taccatcagg gtaaaaattt gcatcctgta cgcctgccgc cgccagcttc
     2581 tgaccaatcg cctttatcat tactggcgca agatatccgg ccctgaatat gcgcacggat
     2641 tttctgcctt ccggcacaat ttgcagctta tccagttcgg ataactgctg ctccccaagc
     2701 cacacaggag gctcattatc tccggccata cgcgcgtcat gttcctgcca ttgttttgcg
     2761 tgtgaccagg catcactacc cgcaaaaata atgacttctg tttctttgtg ttttatgccg
     2821 cgtgactgct gttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
     2881 tcttttttat ttcctgaata ttggcgcgtg ctttcttcct gctggttggt ttactgcggg
     2941 gcgctgcctg taccagagaa aaatcacgcc gaaactgata aacaggcatc acgcaatcat
     3001 attcgtagcc ttcacggcgg taggttacgc gccgttcttc cacgccctta atcattaccg
     3061 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
     3121 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gctgttcttt
     3181 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
     3241 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
     3301 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
     3361 gtatattgtt tcgtggtaaa tatcacgcat ggcttacatc ctcaggaaat tttcttctgt
     3421 aatgcgcctc tgccacatat tccgcataat cggcggcgat attcagtaca tcaagccccg
     3481 ttgatttata ttctctcgtg gaaagtaaga aaaaagccgc tctaataagc tctggcattg
     3541 acgaaagcgc atcagccgca tcatcaggaa cgccggaaaa ttcctgtttc agggaattaa
     3601 aacgatcatc acgcataacc acccccattt tcacaatcag caatcaggat ggctttagcc
     3661 tcattcagcg ccatatcagc actaagttgc ataaccgcca gcgaatgagg aacgaaagcc
     3721 ccggcatatt ctgtttcact ggtgtcgtgc ttatgcgccc tgtcagcaat aacagaaata
     3781 tcaatcagcg catgcattag cgttatgatg gcttcggcgg ctgcgtccgg acgggtgtta
     3841 ttgcacatgg cacacctcct gacgaatacg ggcagcgaat accatcacgc agccagccgg
     3901 agattgctgg cgtgcttcct gttcgctggt ggcctcaatg gtaatcacgc gcggttgtgc
     3961 cgtgctcagg gcgataaaac gccagatgta tttattcagg tcgtgcgagt cccgcccttg
     4021 cgggtgtgtg gtatgatttc tcatagctac ctcgatactt tcgctatcgt tggtggttag
     4081 aagccctgcg agtggtaacg acacttgcgg ggctttgcat ttatgcacct tgataatctc
     4141 aaggtgtggc ccactatatg ctttaggtgt ggcccacatc aagggtttta tttgtgcttt
     4201 ttctgtgtat actgtccccc accaatccaa cagaggaata gaaatggcaa cgggtacaac
     4261 aaacgcaaaa tcacaagctc taaaggctcg tgtaccacac gaaatagtaa acgccatgga
     4321 atcagtgaaa gaatcaggcg aaagcacatc acaattcatc attgcgtcaa tgcaaggcga
     4381 gatcaaacgc cgccagcgcc gcaaggccaa agaatcagaa taatcactat cagcgccgtg
     4441 gtgtgaggta ttacggcgca ttgctatgca ggacaacaca atgaccgata aagaattgac
     4501 caaaacatta tcaccggcac ggaaaagacg gcgcagaaag atagaacatg aatcagaaag
     4561 attcgcgcca tgtgcttttg cccttgagca attccttaaa gagtacaggg aaaagcgctc
     4621 attgcaggta tggcaacgaa ctgaaccaga ctgattgcat tgcccaccag cctgatagcg
     4681 gctatcatcc ccgtgcttat gtttgggatc acacacacaa acggcgcagc gggttatctg
     4741 ttcagaaagg cggctccatt tcggggccgc tttttttatg cctgaaaaac cccaattttg
     4801 tggttttcca gtctcaccag ggcgaacgaa tccccgccca cgttctggcg tatattcaat
     4861 cttcatggtt atagctctgt gttcagatga ttgatgtgtg gcggctgtgt gccgccagcg
     4921 tgattaatga actgccttgc agctatcctt ccaggccaga acctcggata aagaccagcc
     4981 aacggaacga ccgccaagtt tacgacgtga tgggaattgt ccggcctttt ccaggcggta
     5041 gcggcatgag cggctaaggc ctgttagctt ttcgcattct ttttcacgta taaaccgatc
     5101 agtgcttaac actattgccc cctttcgttt cttaaagagt tatttcgtgt tctattgcgt
     5161 tgggatgtgt ctgattgtgt caggatgatt cagagttggc aaatgttgag gtcgtatggt
     5221 ttacagaaac aggaataatc aggataaaat catttaaatt catgttaata caaaggcata
     5281 aaatattgtt tcatgccttt tttctcgcca tttaaagagt gattcgctag tgtataaaaa
     5341 accagtaaca cattaaaaat cagccactta taaatccgta cactttttcg cctcttgttc
     5401 gtagttgttc cgcattgttg ctcattgttg catgttgtat ctgttcgcat atccagtatg
     5461 cgcatactga aaaaacacga aaaaaattat tttcttctgg ctactggtag cgtggttacg
     5521 ttttcatgtg ttcccgccag tatccctaac cgctccgtcc acatatccag cgcattgcgt
     5581 ttagcatcca gataacggga atgattataa actcgctgca ttcctggcat ctgatgacct
     5641 gtaagctgct ccacgacatg cggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg
     5701 cgccggatgt catgcagtga ccagtgagga tgattaagcc tcctgtgcgc taatcttcca
     5761 tactgcgata cgctggcctc ctgtttcgct tcccccagca ataagcccgt gtgcctgttc
     5821 tgctccacca gctgcgtgac gaacggcagg atcgcttccg gtatgggccg gaatattgcg
     5881 accttcgttt tgctgtgctc cttcggaacg gtccatagca tttccgtaaa atcccactcc
     5941 ccgatctccg atagcctcag ttctaccgtc ctggctccga agacaatcag gaggcggatt
     6001 aacgcgacgt agtaagggga aaatattttt ttgtccagtg cctgcaataa ttcgccaagt
     6061 tctttgttac ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc
     6121 agatcgtcca gaacgttgct gattgcatag cgccgcctac ggcagaactt aagcgcctgt
     6181 ttgcacgcct gtagcacgaa tccggcagta acaggcgttc gctttgccac ctggtcaaaa
     6241 caggccagcc agtgccgtag ctcgcattta tccagcggca tagcaccaat ctgctgtatt
     6301 acgtgattat taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtaa
     6361 tactcaagcc agtaggtgag cgcatcgcca accgttacgg gctttaacgc ttcctgtacg
     6421 gtgtaattca tctcatgacg tggatttttc ccctcagcca gccatgtgcg acactgtgcg
     6481 gctttttccc tggctgcttt caggctcaga tcaggataac ttcccagctt aatgcgttcg
     6541 ggtcgtgtct cccttcccgt tccggccctg tatgtgaaat accaggtcaa tttccctgat
     6601 gttaaatatt tcacgctcag gtttccgcca tcactataaa acgtgttttt ctccgctggc
     6661 ttaccatgaa gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt
     6721 taccccctct ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg
     6781 ctacaacgtg aaacaagctg gaacaaggaa aatcacaaat ctgttgataa tcaaaaacat
     6841 aatgcacagc ctgaaacatt atgaaacagc aaaaaacact aaatgataaa atgctgttat
     6901 tcttaacccg ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc
     6961 gattttgatg accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc
     7021 gctcaacgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     7081 agctcttgag ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca
     7141 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     7201 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc
     7261 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     7321 tcaacgtgca attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat
     7381 caccggcctg gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg
     7441 gggattgctg gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat
     7501 cccccctatc gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     7561 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     7621 tgggctgggg ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     7681 aggaccggtg ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga
     7741 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     7801 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     7861 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     7921 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     7981 caggcatacc agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     8041 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     8101 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     8161 aacgccccgc aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct
     8221 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     8281 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     8341 gctcgcccac ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     8401 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg
     8461 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag
     8521 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac
     8581 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     8641 atgccagcac ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg
     8701 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     8761 aaatcttgcc acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga
     8821 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     8881 cgtcatgatg cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctat
     8941 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac
     9001 caattgcccc accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc
     9061 caaaaatggt tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc
     9121 gagacgtttc atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa
     9181 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     9241 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     9301 gcgataaaac taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca
     9361 acaataatcc ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcatgcgat
     9421 acattccaca ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca
     9481 agaaactctt tcggcgcaac gcttgccagc caaccgaaaa ggatgattgc cagcgccatc
     9541 aatgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     9601 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     9661 tcgcccgcac ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata
     9721 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc
     9781 tgtgaggatt gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca
     9841 ttttccgtag tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct
     9901 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     9961 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
    10021 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
    10081 ccatcgccgc tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt
    10141 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
    10201 tttgctgcgc caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc
    10261 ggtggcactt tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc
    10321 gcttcaacaa tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat
    10381 tgtgcgcgtg agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca
    10441 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca
    10501 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
    10561 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
    10621 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
    10681 tgttccactg tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
    10741 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
    10801 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
    10861 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
    10921 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
    10981 cgccggataa agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc
    11041 acgaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat
    11101 gctgatacgg ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa
    11161 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
    11221 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
    11281 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
    11341 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
    11401 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
    11461 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
    11521 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
    11581 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
    11641 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
    11701 aaagcagcaa aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac
    11761 cagggcggca accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
    11821 gtccagagcc cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
    11881 ggtggcgaag ccgcgaagaa agttgagatc ccaggtgttg cgactaccgc atcaccaagt
    11941 tctgaagtcg gtcgtttctt tgaaacccag tcatcaaaag gcggacgtta cactgtcacg
    12001 cttcctgacg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
    12061 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
    12121 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa
    12181 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
    12241 aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat
    12301 gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
    12361 tgccctaata tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac
    12421 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
    12481 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
    12541 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc
    12601 ccgaacttga cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
    12661 tttgttccgc atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga
    12721 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg
    12781 gtaatacctg gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga
    12841 tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt
    12901 tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc
    12961 tcgacttcac cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc
    13021 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
    13081 gtaataaacg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
    13141 acttgctggt aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc
    13201 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca
    13261 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg
    13321 cagaagttcc gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga
    13381 acgcacaagc tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc
    13441 tggtactcat ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa
    13501 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
    13561 tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg
    13621 gtccaatgca cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt
    13681 atgcgcgcaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
    13741 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatacc
    13801 atcgttggat agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta
    13861 ctggcggagg tcgtcgcatc ggcctcgccc tcgcctggca tttcattaat caaaagcaac
    13921 cggtgattgt cagctatcgg acgcactatc cagccattga tggactgatt aaggcaggtg
    13981 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
    14041 tactaaaaag cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg
    14101 aaaaaccagg tacaccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata
    14161 ccccatacct gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca
    14221 gcgatatcat tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catgttgcgt
    14281 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
    14341 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
    14401 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
    14461 aagtgatcga cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtttcc
    14521 cacttgatgg cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc
    14581 atgcgcttat cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc
    14641 cggtgaaata ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa
    14701 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
    14761 taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
    14821 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
    14881 tctttttacc ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt
    14941 tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc
    15001 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt
    15061 gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc
    15121 gaagaaacca ttttgcgaga aagtccggat ttggtgttac tcgacatcat gctaccaggc
    15181 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt
    15241 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
    15301 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
    15361 cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa
    15421 gccctgcatt tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac
    15481 actgaaatct cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc
    15541 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    15601 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    15661 gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca
    15721 tgggaataag cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc
    15781 cttgtgatgt ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa
    15841 cagtcgctgg atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag
    15901 atccccccac acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat
    15961 ctgcgtgtcg agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt
    16021 ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc
    16081 cactacgtgc tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg
    16141 ctggatatcg ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg
    16201 atgcgtccgc actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat
    16261 gggcatctca atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc
    16321 gcatttaacc agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt
    16381 gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg
    16441 agcgataacc tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa
    16501 gctttaattg aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat
    16561 cttagcgaac cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta
    16621 acgcccgata aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat
    16681 atgcgcttaa tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat
    16741 tcaacggttg aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat
    16801 gatggcccag ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc
    16861 gatcccagcc gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct
    16921 atagcactgg caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc
    16981 ttctcgttta gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac
    17041 gcgcacgatg gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg
    17101 gttaatatta tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg
    17161 gaacaagagc tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc
    17221 gtgttctctt tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag
    17281 gtaaaacaac atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta
    17341 tttattcaac aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg
    17401 ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc
    17461 aataaactca agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg
    17521 gcacgttttg aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc
    17581 acgctcaccg ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc
    17641 attaagaatt tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca
    17701 cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag
    17761 gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag
    17821 ccgattgccc gcgtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca
    17881 ccactgattg cactgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg
    17941 ttaaattacg atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt
    18001 ttgatcattc cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg
    18061 accatctgtt ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca
    18121 agggccgcag cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctccgcggct
    18181 ttgtcataac caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat
    18241 tgattgattc gctcacgatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc
    18301 atgccatctg ccagcaagcg caccgattgc aggaaattgt ggatcaccat tggacggaag
    18361 acgttcagtt caaagttacc ggaagcgccc cccatgttga tcgccacgtc gttccccatc
    18421 acctgacagc agagcatggt taatgcctca cactgtgttg gattcacttt ccccggcatg
    18481 attgagctgc ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca
    18541 gaggccagcc agcggacatc attggcgatt ttcatcagtg acacagccaa ccctttcaac
    18601 gcgccgtgcg cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg
    18661 gtaacaaacg gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc
    18721 ggatgggtat ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga
    18781 ggcaggctgt attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc
    18841 tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat tttgacgata
    18901 tcggcaaatg cacgcgattt ttcactcagt gtctgtgtca gggttttaag ctgcggaatg
    18961 agttgcttgc gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg
    19021 gaactttggc ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg
    19081 ccgccgagta attcactggc ccggttagcc agcacttcgt tcatgttcat gttactttgc
    19141 gtgccggagc cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt
    19201 acttcatccg ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct
    19261 tcattaactt ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc
    19321 attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc
    19381 ttatctgccg ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg
    19441 gcctgctcct cacctgttta attttttctt tctgttttgc tttcgttaag caacttttag
    19501 ctcacttatt atttactatt tgataacaaa tgtttggtct ttcgtgccat gtaaaaaaac
    19561 cgccccgaag ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg
    19621 ctggaagaag tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc
    19681 aattttccag atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat
    19741 acttccctgc gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg
    19801 ttttttacag gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact
    19861 tccgccctgc gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc
    19921 aagagaaccg gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg
    19981 atctttaatg tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg
    20041 agcaatatca cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata
    20101 ctgcgacaac tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc
    20161 gccttctccc gctttacgca gctcttcagg gatatattta cctggattat gttccagttt
    20221 ttcgatccag atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga
    20281 aacacccata ccaaccggac aggatgcgcc gtgacgtggc aggcgaatca cgcgtatgtc
    20341 gtgagcgaag tatttaccac caaactgcgc acccagaccg agattttgcg cttcgatcag
    20401 caattctttt tccagttcca catcgcggaa tgcctgaccg tgctcattcc cttccgttgg
    20461 cagttcgtca tagtatttag cggaagccag tttcactgtt ttaaggttag tttctgcaga
    20521 agttccacca attacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg
    20581 catcttctca accaggtaat ttttcagttt ccccggcgtt agtaacgctt tggtttcctg
    20641 atagagatac gtcttgtttg ccgaaccacc acctttggcg atacagagga atttgtactc
    20701 gtcgccatca acggcataaa gatcgatctg cgctggcaga ttggtgccgg tattcacctc
    20761 tttatacata tccagcggcg cgttttgcga gtagcgcaaa ttatcttcga tataagtgtt
    20821 atagacaccg cgcgccagcg ccgcttcatc acagccaccg gtccatacac gctgcccttt
    20881 tttaccaaca ataatcgcag tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc
    20941 gatgtcggag ttacgcagga attgcagcgc cacatattta tcattttcgc tggcctccgg
    21001 gtcacgcaga atgtcggcca cctgttgttg gtgagccgga cgcagcataa acgacgcatc
    21061 atgaaacgcc tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg
    21121 cccttcaaat tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc
    21181 ttttttgagt ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact
    21241 tactgcctgg tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa
    21301 caatatccta cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag
    21361 gttacttccc gtaggattct tgctttaata gtgggattaa tttccacatt aaaacaggga
    21421 ttgatcatgc aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg
    21481 ttgactgaac tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg
    21541 ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg
    21601 cgtgatgtga ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt
    21661 ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt
    21721 catccaaaca aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg
    21781 atggatgccg ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg
    21841 ctgacgcctt tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctactc
    21901 cagccagtcg caggtgcaca tccggcaatt gctcactttt tacaacagcc taatgccgaa
    21961 cgtttaagcg aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg
    22021 ctggcgattt taaaatcggc gctcgacagt cagcagggtg aaccgtggca aacgattcgt
    22081 ttaatttctg aattttaccc ggaagacagc ggcctgttct ccccgctgtt actgaatgtg
    22141 gtgaaattga atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg
    22201 caaggcgtgg cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg
    22261 cctaagtaca ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct
    22321 aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg
    22381 gatgattttg ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag
    22441 agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag
    22501 ttacagctta aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc
    22561 aaaggccacg gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt
    22621 aacatctctt gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga
    22681 tttcatttta tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc
    22741 gctaggcgta gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct
    22801 cgaagacatg gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct
    22861 ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt
    22921 gaaacccatt gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa
    22981 cgaatcggtt gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc
    23041 gtcgatggca tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga
    23101 tatggcaaaa ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc
    23161 cagttccgat atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt
    23221 tagcggcggc gagttccagt taaatgctga cagagacggc aaagccatct ccctttccgg
    23281 ggaggcgcaa agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac
    23341 ctttaataat ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg
    23401 aaaccaaaaa ctgtcactgg aaaaaatgac catttcagtg gaaggcaaag aactggcact
    23461 gctggaaggc atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa
    23521 tagccaactg gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg
    23581 caagctgact ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca
    23641 atataacgcg caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact
    23701 ttatcaggag aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga
    23761 tccggtgatt actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa
    23821 tctgtcgctg ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca
    23881 ggaagtagat cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc
    23941 aactgagttt atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa
    24001 actggcgaaa cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac
    24061 cttgcaggac aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa
    24121 cgggcagaaa atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt
    24181 tccggtcgta cccgctattc cgcagcagta attcacagac aaaacgccgg agatttttct
    24241 ctccggcgtt attttttact tcagcataaa gtcatactga atactcattt tcacatccca
    24301 gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc
    24361 aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa
    24421 gatacccgtg ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat
    24481 cggtgcaaat gtcagcgtaa attgcggcgt taaacgatag ttgacgccca attgccagga
    24541 ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc
    24601 caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca
    24661 cggcgcattc caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt
    24721 acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt
    24781 cgggtcgagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg
    24841 taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga
    24901 acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat
    24961 accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat
    25021 atttggtgtt aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg
    25081 cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc
    25141 gaagcgacct agtttaagca ggctattttc actgggtttt atttttactg cccccagggc
    25201 aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc
    25261 taatttataa acataatatg ccccgccttc aacgccaata atattggaat aataaccact
    25321 attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact
    25381 gggcttatcc gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc
    25441 agccagttgc gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc
    25501 catggccact atttttctca tagttgcacc tttgatgttc tgattatttt tattgaatat
    25561 taattagtga tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc
    25621 acgatttctt tgaatttttt atccgtgagc ggataaaacc agataataac gaatgccagt
    25681 agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc
    25741 gtttgcacct gattggcgat atatccgctt aatcccaaaa taaaggcagg aattgaacct
    25801 ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt
    25861 cgcacgccgg tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcaca
    25921 gtcatagtaa cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt
    25981 gaccagacgg aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc
    26041 aggaaggtat tctttttacc gatcctcgcg accatccccg gcaccagcgg tgccgatgcc
    26101 acagtaccaa ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac
    26161 acatagcgca cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac
    26221 agcgcaccga tgcacaacat aaacagcggg cgattccgtt tcagggtttg cagactgatc
    26281 ttcaatgacg gctgcgccac gatacgtacc acattctcac gcgtcgattt gaagcagatg
    26341 aagtaaagca ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc
    26401 atctcttccg ggctggagtt cttaatgctc ggtcctatca gaaatgccag gcagacaaag
    26461 gttaatgaag cggcaatccc gcgagccgcg cccagacggg cgcgggattg tggttgttgg
    26521 gtcatcgcgg tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg
    26581 cccatgtagg tcaaatatgc atacaccact ttgctaccat ggctccagtc ggtcggcacc
    26641 cagaatacca gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg
    26701 aattttcccc agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag
    26761 gcatcgaata cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg
    26821 acgtcggtgt agtaactcaa taggaagagc gcccccattg cgaaggcgaa gttattggcg
    26881 acgtcaccga ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc
    26941 ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag
    27001 ccgccgactt cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca
    27061 atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc
    27121 tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc
    27181 acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga
    27241 taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg
    27301 ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg cacagcacat
    27361 caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga
    27421 tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg
    27481 caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt
    27541 ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga gtttccccgt
    27601 tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta
    27661 aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg
    27721 cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa
    27781 tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca
    27841 agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga
    27901 actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact
    27961 ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga
    28021 ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca accacctgtt
    28081 gctccgcatc acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag
    28141 acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt
    28201 tcggcgtggt gtagagcatt acgctgcgat ggatcccggc atagttaaag aaatcatgga
    28261 agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct
    28321 gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat
    28381 acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat
    28441 tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc
    28501 ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc gcataattac
    28561 gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt
    28621 cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga tccagactga
    28681 atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca
    28741 taagggactc ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa
    28801 ttctgtgttg cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga
    28861 actgacaccg cgatcaaaaa accaaaaact gcaataccca cagcgtgata ttgatcgcat
    28921 taatctttaa aacggcgttg acagaagcca taaaataagc aaccgaacgt tcgttcgagc
    28981 atattgggaa cgataaactc tcatgacata tggctacggt gaatattttt gggggggaat
    29041 tctccgatcc cggctagatt gtattttgta cagggaaatt aaagtatgaa acgcgggcac
    29101 agttatcacc agcccgcgaa atgagaggat caggatgcgg ttaagatacc gccaatcatc
    29161 gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat cttccgcttc gatatcggcc
    29221 agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca ttgacgtgtt gatgtctggg
    29281 cttatctcgc ctttttgctg ggcatcacgc atccgctcgg caacaaattc agtgatcgtc
    29341 atatgcttat ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt
    29401 tcgaccacca gcgcccgttg cccaaaggct tcatgggtga gagagacaat agactcgacc
    29461 atatagtcaa caaaatgaat cccttcaatc ggttcccgga aacgggccag cgccctctcc
    29521 tggtcctgta agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc
    29581 gtcccgggac taatggcgca ggatttacag atggctttca tcgaggcact gtgaaatcca
    29641 ttttctgaaa aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca
    29701 gtctgcatgt tatccatcat aaaaccatta tagttagcgt gaataactag tttaccgcgt
    29761 tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa ggcacactct attcgtatac
    29821 ttttcactct gaaattggtt aaccaatagc cgtgatttgg ctgccagtta tttagcagag
    29881 acgttgatga gtttatcccg gtaaacaaca cgcacggttg tttaccggtc cgttagtgta
    29941 ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct
    30001 cgcagcaggc gagcaaagga acagcgctgc gttagcaata tcttgcggtt ggcccagacg
    30061 tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag
    30121 ggcatcggtt aatattgccc ccggcgcaat gccatttacc cgaatatttt tttcacccag
    30181 gtcaaacgcc atatttctga ccagatgact ggctgcagct ttagatgatg cataggaagt
    30241 catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc
    30301 atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag
    30361 ttcataagca cggcgaaaat ccgccattgg catatcaaac ggtttaggtc caccgccacc
    30421 ggcgttgtta accagaatat caactttacc cagcttactg atagcaaagt ctgccagtgc
    30481 agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgac cacccagttg
    30541 ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga
    30601 tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt
    30661 gatgatggcg cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg
    30721 tgaacttcag taaaaataaa aggcaccgcc tgtgtgccac attttttatc attaaccttg
    30781 aggaaaggat actaccaggt atagcagata aagagaggat tatttcgccg caatgagccg
    30841 ggcgggaata ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg
    30901 atccgcaagt gtgataccaa gttcccgcgc tggcgtgctg gcccaggtaa caggtatatc
    30961 atcaagtgtg gttggtgtcg catcggtaaa tgccgccagc gaaacctgtt gctcaaagta
    31021 acgatcgact ccgctttccc cgctttgccg ccctgctttg agcaaaccaa accatgcccc
    31081 catcgcaata gtttcgttat agcaaaccac ggcactgatg gtcgggttat gacgtaaaag
    31141 cgccgtgata gcttccgcgg cttgcttctg gctggaagtg cactccaaca cccaatcgct
    31201 atgaaacggc aggccaaatt ttagtagagt tgcacaatag ccccccaccc gttctgcacg
    31261 ggttaatgag gaactttgcc ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag
    31321 atgctccgtc aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacatcatc
    31381 aagataactg gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg
    31441 caggtcatcg ctgcttcctg cagccccggc aattaccaca ccatccacac cctgattcag
    31501 taacagtgaa aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac
    31561 catccgtccc tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg
    31621 cgcagataaa tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga
    31681 cgcctggcga ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt
    31741 ggcggtagag attcgccctt tgccactaag caccagcgaa acggtactta ccgacacgcc
    31801 cgcagccagc gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta
    31861 ctccctgatt atgtgacaga taaaacgttt taccttttat ttatcttata ctcgctatta
    31921 tcgttgcgta atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg
    31981 caatttactg aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca
    32041 ccgaaagtca cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg
    32101 gcattattgt cgttctgcgg cattatgctc ggcattggta gttctcttag tagccacgat
    32161 gtcatcaccc tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg
    32221 agtaagattg gctcgtttgc ttttagtttc ctgccagtca tgttctgtat cgccatcccg
    32281 ctgggtctgg cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggttatgcg
    32341 gtaatgaacc tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggac
    32401 gccgcggttc tgaaagccaa taacatccag agcattcttg ggatccagtc gatcgacacc
    32461 ggtatcctcg gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgattccat
    32521 aatatccgcc tgccggatgc gcttgcattc ttcggcggta cgcgcttcgt accaatcatc
    32581 tcctcgctgg tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcg
    32641 atgggtatta gcggcttggg ccatatgata aacagcgcgg gtgatttcgg accgatgctg
    32701 tttggtaccg gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta
    32761 attcgcttta ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca
    32821 ttgaccatct tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc
    32881 acgcgtttcc tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg
    32941 ttagctatgt atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt
    33001 tctggcctga tcgcctgcgt cgttggcggc actaccgaac cgctggaatt cctgttcctg
    33061 ttcgtagcgc cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc
    33121 atgtctgtgc tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg
    33181 ttcggtattt tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc
    33241 tggtttgtcg tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc
    33301 ccggggcgcg atagcgaagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt
    33361 aaatcaggtt acaacgttcc tgcaatcctc gaagcattag gcggtgccga caatattgtc
    33421 agcctcgata actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat
    33481 gtgctggcac tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg
    33541 caggttgtta tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat
    33601 actgtccagg cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc
    33661 acatggtgta cacagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg
    33721 ttcacgattt cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag
    33781 cgcctgatgc acggcgtatt tggctacagc cgctggaaaa acgatgagtt tctcgcggct
    33841 attgcccact ggttttccac ccagcattac accgccatcg attctcagac ggtggtgtat
    33901 ggcccttctg tcatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa
    33961 ggcgtggtga tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag
    34021 cgcacagtaa tgcccgttgc tttagagaag caggctgatg gttggttttg cgatatgggc
    34081 aagttggaag ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag
    34141 aatcctaccg ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctatgcgag
    34201 cgtcatggtg tgcgggttat ttccgatgaa atccatatgg atatggtctg gggcgagcag
    34261 ccgcatattc cctggagtaa tgtggcgcgg ggagactggg cgttgctgac gtcgggctcg
    34321 aaaagtttca atattcccgc gctgaccggt gcttacggga ttatagaaaa tagcagcagc
    34381 cgtgatgcct atttatcggc actgaaagga cgtgatgggc tttcttcccc ttcggtactg
    34441 gcgttaactg cccatatcgc cgcctatcag caaggtgcac cgtggctgga tgccttacgc
    34501 gtctatctga aagataacct gacgtatatc tcagataaaa tgaacgccgc gtttcctgaa
    34561 ctcaactggc agatcccgca atccacttat ctggcctggc ttgatctacg tctgttgaat
    34621 attgacgaca acgcgttgca aaaagcgctt atcgagcaag aaaaagtcgc gatcatgccg
    34681 gggtatacct atggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt
    34741 tcgaaactgg aaaaaggtgt ggctggatta ataaacgcca tccgcgctgt tcgctaaccc
    34801 caattgcgca acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta
    34861 taatggtgcg cacttttata ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc
    34921 attaactgat atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact
    34981 tggccgccag tataatatct cgcttcctgc acaatccctg gaaacactga ttccccacgt
    35041 tcaggtcatt gccaacgaac ccgatctggt gagctttctg accaaacttg actggggcgt
    35101 taaagttctc gcctctcttg atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc
    35161 agcccgtcac ggcctgcact atgtcgagct gcgtttttca ccaggctaca tggcaatggc
    35221 acatcagctg cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg
    35281 ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg agccggacct tcggcgaagc
    35341 cgcctgtcag caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga
    35401 tttagccggt gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccgcgc
    35461 gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat
    35521 ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga catggcgtaa aagccattga
    35581 agatcgggcg ctgatggatt ttctcgccga gcaacaaatt ggtattgaat cctgtctgac
    35641 ctccaatatt cagaccagca ccgtagcaga gctggctgca catccgctga aaacgttcct
    35701 tgagcatggc attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat
    35761 cattcacgaa tataccgttg ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca
    35821 ggcacagatt aatggtctgg aaatggcttt cctcagcgct gaggaaaaac gcgcactgcg
    35881 agaaaaagtc gccgcgaagt aacaaaatgg atggtgcaaa ttgcaccatc catttttcat
    35941 gcaaggcaca aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt
    36001 acctggattg cctggcttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga
    36061 atagccgcat agtaagccgg ataattccca ggtaccgtca acagcgtttc ttcgacacgt
    36121 tcctcacctt ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc
    36181 ggcagacgct cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa
    36241 ctgcctcggg atccatgcac gatataccgt gctgactcag cggctgccag catggtaccg
    36301 tgtaaaatga ctcgccgctg tggataggac aagatggcgt ggaaataatc ggtcgactgc
    36361 gctccgggcc gtaactgtgc caaatctacc gtcatgctga ccggtaaacc aaaaagcgta
    36421 atggcctgat caagaagatg tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc
    36481 tgttcacgcc aacgatcgcg cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa
    36541 gcaacttcac ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc
    36601 cagcgacggt tatggaatac agacagcaca cgccccaggc tttttgccag cgcatccagc
    36661 tctcgcgctt gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc
    36721 tcaagcgccg ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg
    36781 tctatgttgg gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag
    36841 tcggctttta cttttgtttc atcactgctg gagattaccg ccagttccag cccgggcgtg
    36901 cccgcaatca ggggcgcatg gaaggttttg ctcgcataac catacccaat caacccaaca
    36961 cggatgttgt cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc
    37021 ctttacccac gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa
    37081 gtcaaaaaaa tagcgaattt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa
    37141 aatcttgctg gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc
    37201 aagagagatg tgggctgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt
    37261 cgtttcagtc attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca
    37321 gagtgcttcg gtacaacaca agtaagctct gcacttgtgg agcgacatgc tgcccgtccg
    37381 ggtgcatgtt ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcgatgttgt
    37441 tggtgattgt cagggatagt aaagcgcgtt actcttttca gagcagtttt tcatccctga
    37501 atatttgcca tcagcgcatt cttattattg taataacaca agcgttcact tttggtgaaa
    37561 ttttatcatc actaataata cctacccctt tggtattaat tattttatgg accttttatg
    37621 actgttcagg actacttatt aaaatttcgc aaaatcagtt cactcgaaag tctggaaaaa
    37681 ctctacgacc atcttaatta caccctgacg gacgatcagg aactgatcaa tatgtatcgt
    37741 gctgccgatc accgtcgcgc agagctggtt tctggcgggc gtttgtttga cctcggccag
    37801 gtaccgaagt ccgtctggca ctatgtccaa taaagaaagt agcgatcggt tataaaagcc
    37861 ttataataac gcccctaaaa ttagatttgc ctgtttgcgt cgctattctg gagaacctat
    37921 gaccaccaca acgccacaac gtattggagg ctggttgctt ggcccgttgg cctggctttt
    37981 agtcgctctg ttgagtacga cgctggcgtt gttgctgtat accgctgcgt tatcttctcc
    38041 gcaaacattt caaacgctcg gcggacaagc actgacgaca caaatcttat ggggcgtttc
    38101 ttttattacc gctatcgcca tgtggtatta cacactgtgg ttgaccatcg ctttctttaa
    38161 acgtcggcgt tgtgttccta aacactatat tatctggctg cttatttccg tattgctggc
    38221 agtaaaagca ttcgcttttt cacccgtgga agatggcata gccgttcgcc agttgctgtt
    38281 tactttgttg gcaaccgcat tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc
    38341 gacgtttgtg aatccgtaat aaccttacag ttaacctgtt gtcgcctgct ctggattaac
    38401 ggataatagg cggctttttt atttcaggcc gaaaaatgac tgactacctg ttactgtttg
    38461 tcggaactgt actggtcaat aactttgtac tggtcaagtt tctcggtctc tgtccgttta
    38521 tgggggtttc caaaaaactg gaaaccgcga tgggcatggg gctggcaaca acgtttgtga
    38581 tgacgctggc gtctatttgc gcctggctta tcgatacgtg gattttgatc ccacttaatc
    38641 tgatttacct gcgcaccctg gcatttattc tggtgattgc tgtggtcgtg cagttcaccg
    38701 agatggtggt gcgcaaaacc agcccggtgc tttaccgtct gctggggatt tttttgccgc
    38761 ttatcaccac caactgtgca gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca
    38821 atttcttgca gtcggcgctg tacggttttt ccgccgctat cggtttctcg ctggtgatgg
    38881 tgctcttcgc cgccatccgc gaacgccttg ctgtggctga tgtcccggca ccttttcgcg
    38941 gtcatgccat tgcgttaatt accgcaggtc ttatgtctct ggcctttatg ggctttagtg
    39001 gtttggtgaa gttgtaatga atgctatctg gattgccgtt gccgccgtga gcctgctggg
    39061 cctggcgttt ggcgccattc tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc
    39121 ggtcgttgag aaaattgacg aaatcttacc gcagagccag tgtggtcagt gcggttatcc
    39181 cggctgtcgc ccctacgcgg aagccatcag ctgtaacggt gaaaaaatca accgttgcgc
    39241 cccaggtggc gaagctgtga tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc
    39301 gctggatggc gaagcgcaag agataacgcc tgcgcggatg gtggcggtta ttgatgaaaa
    39361 taactgtatt ggctgcacta aatgtattca ggcgtgtccg gtagacgcca tcgttggcgc
    39421 tacccgtgcc atgcatacgg taatgagtga tctctgtacg ggctgcaatt tatgtgttga
    39481 tccgtgcccg acgcactgca tctcgttgca accggtcgca gaaacacctg actcctggaa
    39541 atgggatctg aacaccattc ccgtgcgtat cattcccgtg gaacaccatg cttaagttat
    39601 tctctgcatt cagaaaaaat aaaatctggg atttcaacgg cggcatccat ccaccggaga
    39661 tgaaaaccca gtccaacggt acacccctgc gccaggtacc actggcgcag cgttttgtta
    39721 ttccactgaa acagcatatt ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag
    39781 tattgcgcgg ccagccgctt acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca
    39841 cctcgggtac cgttacggct attgcgcccc actctacggc tcatccttca gctttagctg
    39901 aattaagcgt gattattgat gccgatggtg aagactgctg gatcccgcgc gacggctggg
    39961 ccgattatcg cactcgcagt cgcgaagagt taatcgagcg catacatcag tttggtgttg
    40021 ccgggctggg cggtgcagga ttcccgacag gcgttaaatt gcagggtggc ggagataaga
    40081 ttgaaacgtt gattatcaac gcggctgagt gcgagccgta cattaccgcc gatgaccgtt
    40141 tgatgcagga ttgcgcggct caggtcgtag agggtattcg cattcttgcg catattctgc
    40201 agccacgcga aattcttatc ggcattgaag ataacaaacc gcaggcgatt tccatgctgc
    40261 gcgcggtgct ggcggactct aacgatattt ctctgcgggt gattccaacc aaatatcctt
    40321 ctggcggtgc taaacaatta acctacattc tgaccgggaa gcaggttcca catggcgggc
    40381 gttcatccga tatcggcgta ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg
    40441 ccgttattga tggcgagccg attaccgagc gtgttgtaac cctgactggc gaagcaatcg
    40501 ctcgcccggg caacgtctgg gcacggctgg ggacgccagt gcgtcattta ttgaatgatg
    40561 ccggattctg cccctctgcc gatcaaatgg tgattatggg tggcccgcta atgggcttta
    40621 ccttgccatg gctggatgtc ccggtcgtaa agattaccaa ctgtctgttg gctccctctg
    40681 ccaatgaact tggcgaacca caggaagaac aaagctgcat ccggtgtagc gcctgtgctg
    40741 acgcctgccc tgcggatctt ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac
    40801 acgataaagc taccacgcat aacattgctg attgcattga atgtggggct tgcgcgtggg
    40861 tttgcccgag caatattccc ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg
    40921 ctattcgtca ggaagaaaag cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg
    40981 ctcgtctgga gcgcgaaaaa gcggctcgcc ttgaacgaca taagagcgca gccgttcaac
    41041 ctgcagccaa agataaagat gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg
    41101 cccaggctac acagcctatt gtgattaaag cgggcgaacg cccggataac agtgcaatta
    41161 ttgcagcacg ggaagcccgt aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa
    41221 ctaacgacgc agcaaccgtt gctgatccac gtaaaactgc cgttgaagca gctatcgccc
    41281 gcgccaaagc gcgcaagctg gaacagcaac aggctaatgc ggaaccagaa gaacaggtcg
    41341 atccgcgcaa agcc
//
LOCUS       DAZFDP010000024        40754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFDP010000024 DAZFDP010000000
VERSION     DAZFDP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..93
                     /locus_tag="ACHJ1G_001415"
     CDS             <1..93
                     /locus_tag="ACHJ1G_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4763299.1"
                     /translation="ITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            104..940
                     /gene="eutJ"
                     /locus_tag="ACHJ1G_001416"
     CDS             104..940
                     /gene="eutJ"
                     /locus_tag="ACHJ1G_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4763300.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGGDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            930..2117
                     /gene="eutG"
                     /locus_tag="ACHJ1G_001417"
     CDS             930..2117
                     /gene="eutG"
                     /locus_tag="ACHJ1G_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4763301.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2234..3460
                     /gene="eutH"
                     /locus_tag="ACHJ1G_001418"
     CDS             2234..3460
                     /gene="eutH"
                     /locus_tag="ACHJ1G_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4763302.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3457..4860
                     /gene="eutA"
                     /locus_tag="ACHJ1G_001419"
     CDS             3457..4860
                     /gene="eutA"
                     /locus_tag="ACHJ1G_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4763303.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4872..6233
                     /gene="eutB"
                     /locus_tag="ACHJ1G_001420"
     CDS             4872..6233
                     /gene="eutB"
                     /locus_tag="ACHJ1G_001420"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4763304.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6254..7141
                     /gene="eutC"
                     /locus_tag="ACHJ1G_001421"
     CDS             6254..7141
                     /gene="eutC"
                     /locus_tag="ACHJ1G_001421"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4763305.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7151..7810
                     /gene="eutL"
                     /locus_tag="ACHJ1G_001422"
     CDS             7151..7810
                     /gene="eutL"
                     /locus_tag="ACHJ1G_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4763306.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7823..8323
                     /gene="eutK"
                     /locus_tag="ACHJ1G_001423"
     CDS             7823..8323
                     /gene="eutK"
                     /locus_tag="ACHJ1G_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4763307.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8369..9421
                     /gene="eutR"
                     /locus_tag="ACHJ1G_001424"
     CDS             8369..9421
                     /gene="eutR"
                     /locus_tag="ACHJ1G_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4763308.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9427..10326)
                     /gene="hemF"
                     /locus_tag="ACHJ1G_001425"
     CDS             complement(9427..10326)
                     /gene="hemF"
                     /locus_tag="ACHJ1G_001425"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4763309.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10330..11199)
                     /gene="amiA"
                     /locus_tag="ACHJ1G_001426"
     CDS             complement(10330..11199)
                     /gene="amiA"
                     /locus_tag="ACHJ1G_001426"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4763310.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11413..11838
                     /gene="ypeA"
                     /locus_tag="ACHJ1G_001427"
     CDS             11413..11838
                     /gene="ypeA"
                     /locus_tag="ACHJ1G_001427"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4763311.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11825..12274
                     /gene="yfeZ"
                     /locus_tag="ACHJ1G_001428"
     CDS             11825..12274
                     /gene="yfeZ"
                     /locus_tag="ACHJ1G_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4763312.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12335..12910
                     /gene="yfeY"
                     /locus_tag="ACHJ1G_001429"
     CDS             12335..12910
                     /gene="yfeY"
                     /locus_tag="ACHJ1G_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4763313.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13006..13905
                     /gene="yfeX"
                     /locus_tag="ACHJ1G_001430"
     CDS             13006..13905
                     /gene="yfeX"
                     /locus_tag="ACHJ1G_001430"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4763314.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13963..15267)
                     /gene="pbp4b"
                     /locus_tag="ACHJ1G_001431"
     CDS             complement(13963..15267)
                     /gene="pbp4b"
                     /locus_tag="ACHJ1G_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4763315.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15272..16696)
                     /gene="murP"
                     /locus_tag="ACHJ1G_001432"
     CDS             complement(15272..16696)
                     /gene="murP"
                     /locus_tag="ACHJ1G_001432"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4763316.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16700..17596)
                     /gene="murQ"
                     /locus_tag="ACHJ1G_001433"
     CDS             complement(16700..17596)
                     /gene="murQ"
                     /locus_tag="ACHJ1G_001433"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4763317.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17760..18617
                     /gene="murR"
                     /locus_tag="ACHJ1G_001434"
     CDS             17760..18617
                     /gene="murR"
                     /locus_tag="ACHJ1G_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4763318.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18746..19537
                     /gene="ucpA"
                     /locus_tag="ACHJ1G_001435"
     CDS             18746..19537
                     /gene="ucpA"
                     /locus_tag="ACHJ1G_001435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4763319.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19708..20724
                     /gene="cysP"
                     /locus_tag="ACHJ1G_001436"
     CDS             19708..20724
                     /gene="cysP"
                     /locus_tag="ACHJ1G_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4763320.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20724..21557
                     /gene="cysT"
                     /locus_tag="ACHJ1G_001437"
     CDS             20724..21557
                     /gene="cysT"
                     /locus_tag="ACHJ1G_001437"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4763321.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21557..22432
                     /gene="cysW"
                     /locus_tag="ACHJ1G_001438"
     CDS             21557..22432
                     /gene="cysW"
                     /locus_tag="ACHJ1G_001438"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4763322.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            22422..23519
                     /gene="cysA"
                     /locus_tag="ACHJ1G_001439"
     CDS             22422..23519
                     /gene="cysA"
                     /locus_tag="ACHJ1G_001439"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4763323.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23653..24564
                     /gene="cysM"
                     /locus_tag="ACHJ1G_001440"
     CDS             23653..24564
                     /gene="cysM"
                     /locus_tag="ACHJ1G_001440"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4763324.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24567..24935)
                     /gene="yfeK"
                     /locus_tag="ACHJ1G_001441"
     CDS             complement(24567..24935)
                     /gene="yfeK"
                     /locus_tag="ACHJ1G_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO4763325.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25040..25891
                     /gene="pdxK"
                     /locus_tag="ACHJ1G_001442"
                     /gene_synonym="thiJ"
     CDS             25040..25891
                     /gene="pdxK"
                     /locus_tag="ACHJ1G_001442"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4763326.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25933..26442)
                     /gene="crr"
                     /locus_tag="ACHJ1G_001443"
     CDS             complement(25933..26442)
                     /gene="crr"
                     /locus_tag="ACHJ1G_001443"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4763327.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26483..28210)
                     /gene="ptsI"
                     /locus_tag="ACHJ1G_001444"
     CDS             complement(26483..28210)
                     /gene="ptsI"
                     /locus_tag="ACHJ1G_001444"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4763328.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28255..28512)
                     /gene="ptsH"
                     /locus_tag="ACHJ1G_001445"
     CDS             complement(28255..28512)
                     /gene="ptsH"
                     /locus_tag="ACHJ1G_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4763329.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28896..29867)
                     /gene="cysK"
                     /locus_tag="ACHJ1G_001446"
     CDS             complement(28896..29867)
                     /gene="cysK"
                     /locus_tag="ACHJ1G_001446"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4763330.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30052..30813)
                     /gene="cysZ"
                     /locus_tag="ACHJ1G_001447"
     CDS             complement(30052..30813)
                     /gene="cysZ"
                     /locus_tag="ACHJ1G_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4763331.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31043..32029
                     /gene="zipA"
                     /locus_tag="ACHJ1G_001448"
     CDS             31043..32029
                     /gene="zipA"
                     /locus_tag="ACHJ1G_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4763332.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32100..34115
                     /gene="ligA"
                     /locus_tag="ACHJ1G_001449"
     CDS             32100..34115
                     /gene="ligA"
                     /locus_tag="ACHJ1G_001449"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4763333.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34117..34335
                     /gene="ypeB"
                     /locus_tag="ACHJ1G_001450"
     CDS             34117..34335
                     /gene="ypeB"
                     /locus_tag="ACHJ1G_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4763334.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34332..35330)
                     /gene="yfeH"
                     /locus_tag="ACHJ1G_001451"
     CDS             complement(34332..35330)
                     /gene="yfeH"
                     /locus_tag="ACHJ1G_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4763335.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            35420..36346
                     /gene="yfeR"
                     /locus_tag="ACHJ1G_001452"
     CDS             35420..36346
                     /gene="yfeR"
                     /locus_tag="ACHJ1G_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4763336.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36385..37149)
                     /gene="yfeN"
                     /locus_tag="ACHJ1G_001453"
     CDS             complement(36385..37149)
                     /gene="yfeN"
                     /locus_tag="ACHJ1G_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO4763337.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37398..38231
                     /gene="xapA"
                     /locus_tag="ACHJ1G_001454"
                     /pseudo
     CDS             37398..38231
                     /gene="xapA"
                     /locus_tag="ACHJ1G_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
     gene            38291..39547
                     /gene="xapB"
                     /locus_tag="ACHJ1G_001455"
     CDS             38291..39547
                     /gene="xapB"
                     /locus_tag="ACHJ1G_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO4763338.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            39600..39758
                     /locus_tag="ACHJ1G_001456"
     CDS             39600..39758
                     /locus_tag="ACHJ1G_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO4763339.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            39799..40683
                     /gene="xapR"
                     /locus_tag="ACHJ1G_001457"
     CDS             39799..40683
                     /gene="xapR"
                     /locus_tag="ACHJ1G_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO4763340.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT         9507 a        10252 c        11288 g         9707 t
ORIGIN      
        1 atcaccacgc caaccggtga aggggtaacc agcgcgcgga cgtttgtccg tctgcgtcgc
       61 tgtgtattag tcgatgcgtt tcgcattgtt taaggagtaa ccgatggcgc acgacgaaca
      121 atggctcacc ccacgtctgc aaacggcggc aacgctgtgt aaccagacgc ctgccgcgac
      181 ggaatctccg ctgtggctgg ggggggatct gggcacctgc gatgtggtgt cgatggttgt
      241 tgaccgcgac ggtcagccgg tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg
      301 catcgtctgg gatttcttcg gtgcggtcac cattgttcgt cgccatctcg acactcttga
      361 gcagcaattc ggtcttcgtt ttagccatgc ggcgacttca tttcctccag gtacggaccc
      421 gcgtatttcc atcaacgtgc tggaatctgc cgggctggaa gtgagccatg tgctggatga
      481 gccaaccgca gtcgcggatc tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg
      541 cggcactacc ggcattgcca tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc
      601 caccggcgga catcacatct ctctgactct cgccggaaat cgtcgtattt cgctggaaga
      661 ggcagagcag tacaagcgcg gtcatggtga cgagatttgg cccgcggtga aaccggtgta
      721 cgaaaaaatg gcggacattg tcgcccggca tattgaaggg cagggcatta ctgatttatg
      781 gctggcgggc ggctcttgta tgcaaccggg cgtggcggaa ctgtttcgca aacaattccc
      841 ggcgttacag gtgcatttac cgcagcacag tttgtttatg accccgctgg cgatcgccag
      901 tagtgggcga gagaaagcgg aggggctata tgcaaaatga attgcagacc gcgctctttc
      961 aggcgttcga taccctgaat ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc
     1021 tttgcggtcc gggctcggtg agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac
     1081 atctgttcgt gatggcagac agctttttgc atcaggcagg gatgaccgcc gggctgacgc
     1141 gtagcctggc cgttaaaggt atcgccatga cgctctggcc atgtccggtg ggcgaaccgt
     1201 gcatcaccga cgtgtgtgca gccgtggcgc agttgcgtga gtcaggctgt gatggggtga
     1261 tcgcatttgg cggcggctcg gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga
     1321 acccggatag cacgctggca gagatgtcag aaaccagcgt tctgcaatcg cgcctgccgt
     1381 tgattgccat tcccactacc gccggaaccg gctctgaaac caccaacgta acggtgatta
     1441 tcgatgcggt gagcgggcgt aagcaggtgt tagcccatgc ctcgctgatg ccggatgtgg
     1501 cgattctcga cgccgcattg accgaaggtg tgccgtcgca tgtcacggcg atgaccggca
     1561 ttgatgcgtt aacccatgcc attgaagcat acagcgccct gaacgctaca ccgtttaccg
     1621 acagtctggc gattggtgcc attgcgatga ttggcaaatc gctgccgaaa gcggtgggct
     1681 acggtcatga ccttgccgcg cgcgagagca tgttactggc ttcatgtatg gcgggaatgg
     1741 cgttttccag tgcggggctt gggttgtgcc acgcgatggc gcatcagcca ggggcggcgc
     1801 tgcatattcc acacggtctg gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc
     1861 ggatggtttg tcgcgaacgg tttagtcaga ttggtcgggc actgcgaact aaaaaatccg
     1921 acgatcgtga cgctattaac gcggtaagtg agctgattgc ggaagttggg attggtaaac
     1981 gactgggcga tgttggcgcg acatctgcgc attacggtgc atgggcgcag gccgcgcagg
     2041 aagatatttg tctgcgcagc aacccgcgta ccgccagcct ggagcagatt gtcggcctgt
     2101 acgcagcggc gcaataaatg ccggatgcga cgcttgccgc gtctgatccg gccttcggga
     2161 ttgcacatgt agggcggata aggcgtttac gccgcatccg ccaataaata acaactaaca
     2221 gggagtaaag gcgatgggaa ttaacgaaat catcatgtac atcatgatgt tctttatgct
     2281 gatagctgcc gtagacagga tcctgtcgca gttcggcggt tctgctcgtt tcctcggtaa
     2341 gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa gaaggcttta tggcaatggg
     2401 cgcactgggc ctggcgatgg tcggtatgac cgcgctggca ccggtactgg ctcacgtact
     2461 cgggccggta attattccgg tttacgaaat gctcggcgct aacccatcga tgttcgccgg
     2521 aacactgctg gcgtgcgata tgggcggctt cttcctcgcc aaagagctgg cgggcggcga
     2581 cgtagcagcg tggctatact ctgggttaat tctcgggtcg atgatggggc caacgattgt
     2641 gttttccatt ccggtggcgc tcggcattat cgaaccttct gaccgtcgtt atctggcgct
     2701 cggcgtgctg gcgggcattg tgaccattcc gattggctgt attgctggtg gtctggttgc
     2761 tatgtactcc ggtgtgcaga tcaacggtca gccggtggaa ttcactttcg ccctgatcct
     2821 gatgaacatg atcccggtga tcattgtcgc gattctggtg gcgctggggc tgaaattcat
     2881 cccggaaaaa atgatcaacg gcttccagat cttcgccaaa ttcctcgtgg cattgatcac
     2941 cctcggtctt gccgctgcgg tagtgaaatt cctccttggc tgggaactga tccccggact
     3001 ggatcctatc tttatggccc ctggcgataa acccggtgaa gtgatgcgcg ccattgaagt
     3061 tatcggttct atctcctgcg tgctgttagg ggcgtatcca atggtgctgc tgctgactcg
     3121 ctggtttgaa aaaccgctga tgagcgtcgg taaagtgctg aatatgaaca acatcgcggc
     3181 agccggcatg gtggcaacgc ttgccaacaa catcccgatg ttcggcatga tgaagcagat
     3241 ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt tccgctgctt tcgccctggg
     3301 cgaccactta ggcttcgccg ctgccaacat gaacgccatg atcttcccga tgattgtcgg
     3361 caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg atgctggtgc caaaagaaga
     3421 cgcgaccgcg actaaaaccg aagcggaggc acaatcgtga acactcgcca gctattgagc
     3481 gtcggtatcg atatcggcac caccaccacc caggtgattt tctcccacct ggagctggtt
     3541 aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca ttaaacgcga aattagctgg
     3601 caaagtccgg tgttctttac ccctgtcgat aaacagggcg gtttaaaaga agcggaactg
     3661 aaaaccttaa tactcgagca atatcaggct gcgggtattg cgccggaaag cgttgattct
     3721 ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca atgctcgccc ggcggtgatg
     3781 gcgctctctc aatcgctggg ggattttgtc gttgccagcg ccgggccgca cctcgaatcc
     3841 gtgatcgccg gtcacggagc tggggcgcaa accctttctg aacaacggct gtgtcgggta
     3901 ctgaatatcg acatcggcgg tggcaccgcg aactacgccc tgttcgatgc cggaaaaatc
     3961 agcggcactg cctgtctcaa cgtcggtggt cgcctgatgg aaaccgacag ccaggggcgc
     4021 gtggtttacg ctcataaacc ggggcagatg attgtggatg agtgcttcgg tgcaggcact
     4081 gacgcccgtt cgctgaccgg cgcgcagctg gtgcaggtta cccggcggat ggcggcgctg
     4141 attgtcgaag tgattgacgg aacgctttcg ccgctcgcgc aggcattgat gcaaaccggt
     4201 ttgctgcccg caggtgttac gcccgaaatc attacgcttt ctggaggcgt gggcgaatgt
     4261 tatcgccacc agcccgccga cccgttctgt tttgccgata ttggcccact tctggcaacg
     4321 gcgctgcatg accatccgcg cctgcgtgag atgaatgtgc agtttccggc gcaaaccgta
     4381 cgcgccacgg tgattggcgc gggtgcacat accctttcgc tctctggcag cacaatctgg
     4441 ctggagggcg tacaactgcc gctgcgcaat ttgccggtgg cgatcccgat tgatgaaacg
     4501 gatctggtga gtgcctggca acaggcgctg cttcagctgg atcttgatcc caaaactgac
     4561 gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt acgccgcggt actgacggtc
     4621 atcaacgcgc tggtcgattt cgtcgcgcgt tttccgaatc cgcatcccct gctggtggtg
     4681 gccgggcagg actttggtaa agctctgggc atgttgttgc gcccacagct acaacaactc
     4741 ccgttggcag tcattgacga agtgattgtc cgcgcggggg actatatcga cattggtacg
     4801 cctctttttg gcggatcggt tgtgccggtg acggtgaaat cactcgcatt tccttcctga
     4861 gggaacgact tatgaaacta aagaccacat tgttcggcaa tgtatatcag tttaaggatg
     4921 taaaagaggt gctggctaaa gccaacgaac tgcgttcggg ggatgtgctg gcgggcgttg
     4981 cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt gttgtcggaa atgaccgtag
     5041 cggacatccg caataatccg gtgattgcct atgaagatga ctgcgtgacg cggctgattc
     5101 aggacgatgt taacgaaacg gcctacaacc agattaaaaa ctggagcatc agcgaactgc
     5161 gtgagtatgt gctgagcgat gaaaccagcg tggacgacat tgcctttacc cgcaaagggc
     5221 tgacctcgga agtggtcgcg gcggtagcga agatttgctc caacgcggac ctgatctacg
     5281 gcgcgaagaa aatgccggta atcaaaaagg ccaataccac cattggtatt ccgggcacct
     5341 tcagcgcccg tttgcagccg aacgataccc gtgacgacgt gcaaagtatc gctgcgcaaa
     5401 tctatgaagg gctttccttt ggggtgggcg atgcggtgat cggcgttaac ccggtaactg
     5461 acgacgtgga aaacttaagc cgcgtgttgg acaccattta cggcgtgatc gacaaattca
     5521 acatcccaac tcagggctgc gtactggcgc acgtcaccac ccagatcgaa gcgattcgtc
     5581 gcggcgcgcc tggcggactg attttccaga gtatctgtgg cagcgaaaaa gggctgaaag
     5641 agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc agtgggtgca gagttcaacc
     5701 gtatcgccgg ggaaaactgc ctctacttcg aaaccggaca aggctctgcg ctatccgctg
     5761 gcgctaactt cggcgctgac caggtgacga tggaagcacg taactatggg ctggcgcgtc
     5821 attacgatcc gtttatcgtc aacaccgtgg tcggctttat tgggccggag tatctctaca
     5881 acgaccgcca gattatccgt gctggcttag aagatcactt tatgggcaag ctgagcggca
     5941 tctctatggg ctgtgactgc tgttacacca accacgctga cgctgaccag aacctcaacg
     6001 aaaacctgat gatcctgctc gccaccgcag gctgcaacta catcatgggg atgccgctgg
     6061 gtgatgacat catgctcaac taccagacca ccgcattcca cgataccgcc actgtgcgtc
     6121 agttactcaa cctgcgtccg tcaccggagt ttgaacgctg gctggaaagc atgggcatta
     6181 tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc actgttcttc tgatgacgcg
     6241 gagataacac atcatggatc aaaaacagat tgaagaaatt gtacgcagcg tgatggcgtc
     6301 aatgggacaa acggcccccg cgccgtcaga agcaaagtgc gccaccacca actgtgcggc
     6361 accggtgacc tcggaaagct gcgcgctgga tttaggttct gcggaagcaa aagcgtggat
     6421 tggtgttgaa aacccgcatc gcgcagacgt attaacagaa ctgcgtcgca gcaccgtggc
     6481 ccgcgtttgt accggtcgtg ccggtccgcg tccgcgtacg caggcgttgc tgcgtttcct
     6541 ggccgatcac tctcgttcga aagataccgt actgaaagaa gtgccggaag agtgggtgaa
     6601 agcgcagggc ttgctggaag tacgctccga aattagcgac aaaaatctct acctgactcg
     6661 cccggatatg ggccgccgcc tgtgtgcaga agctgttgaa gcgctgaaag cacagtgtgt
     6721 tgctaatccg gacgtacagg tcgttatttc tgatggcctg tcaactgatg ccatcaccgt
     6781 gaactacgaa gagatcctgc cgccgctgat ggcgggcctg aaacaggccg ggctgaaagt
     6841 cggcacgcca ttctttgttc gttatggtcg cgtgaagatt gaagatcaga tcggtgagat
     6901 cctcggcgcg aaagtggtga tcctgctggt gggggaacgt ccggggttag ggcagtcaga
     6961 aagcctctcc tgctacgccg tttactcgcc gcgtatggcg accaccgttg aggccgatcg
     7021 cacctgtatc tctaacattc accagggcgg cacgccgccg gttgaagccg cagccgtcat
     7081 tgtggatttg gcgaaacgta tgctggagca gaaagcatcc ggcatcaaca tgacccgata
     7141 aggaggcatc atgccagctt tagatttgat tcgaccgtcg gtaaccgcca tgcgggtgat
     7201 tgcctccgtt aacgctgaat ttgcgcgtga actaaaattg ccgccgcata ttcgtagcct
     7261 cgggctgatt tctgccgatt ctgacgacgt cacctacatt gcggctgacg aagcgaccaa
     7321 acaagcgatg gtcgaagtgg tatatggtcg ctcgctgtat gcgggagcgg cacacggccc
     7381 gtcaccgact gccggtgaag tgctgattat gcttggtggg ccaaacccgg cggaagtgcg
     7441 tgccggtctg gatgcgatgg ttgcacatat tgaaaatggc gcagctttcc agtgggccaa
     7501 tgacgcagaa aacaccgcct tcctggcaca tgtagtttcg cgtaccggtt cttatctctc
     7561 atcaaccgcc gggatcacgc ttggcgatcc gatggcgtat ctggtggcac cgccgctgga
     7621 agcgacctac ggcattgatg cagcgttgaa atctgccgac gtacagctgg tgacctatgt
     7681 cccgccaccg tctgaaacca actactcggc agcattttta accggtagcc aggccgcgtg
     7741 taaagcagcc tgtaacgcct ttaccgatgc agtgctggaa atcgcgcgta atccaatcca
     7801 gcgtgcgtaa cggaggttgc cgatgatcaa tgcactggga ttgctggaag tggacggcat
     7861 ggtcgccgcg atagatgcag cggatgccat gctcaaagca gctaacgttc gtctgctcag
     7921 tcacgaagtg cttgaccctg gtcgcttaac gctggtggtg gaaggcgatc tggcggcgtg
     7981 tcgtgcggcg ctggacgcag gttgtgctgc cgcgatgcgt accgggcgtg tcatcagccg
     8041 caaggagatc ggtcggccag acgatgacac ccagtggctg gttactggct ttaaccgcca
     8101 gccgaagcaa cccgtaaggg aacccgacgc gccagttatc gtcgcggaat ctgctgacga
     8161 gttgttggcg ctgttaacat cagtacgtca gggaatgacg gcaggagaag tggctgccca
     8221 ctttggctgg ccgctggaaa aagccagaaa tgcgctcgaa cagctctttt ctgccgggac
     8281 gttacgtaaa cgcagtagtc gttatcgtct caagccccat taacctgtcg gaggtgccgg
     8341 gtgtcctaca acacccggca ttaacatcat gaaaaagacc cgtacagcca atttgcacca
     8401 tctttatcat gaacccttac ccgaaaacct gaagctcacg ccgaaggtcg aagtggataa
     8461 tgttcatcaa cgacagacaa cggatgtcta tgaacatgct ttaacgatta ccgcctggca
     8521 gcagatttac gatcagctgc atccgggcaa gtttcattgt gaatttacgg aaattctact
     8581 cgatgatatt caggtttttc gtgaatacac cggtctggcg ctgcgtcagt cgtgcctggt
     8641 ctggccgaac tcgttctggt ttggcattcc ggcgacgcgc ggtgagcagg gatttatcgg
     8701 ttcgcaatgt ctgggaagcg cggaaatcgc cacccgccct ggtggcactg aatttgaact
     8761 gagcacgccg gatgattaca cgatcctggg cgtggtgctt tctgaagatg tcatcacccg
     8821 gcaggctaac tttttgcata acccggatcg ggtattacat atgctgcgta gccagtcggc
     8881 gctggaagtg aaagagcagc ataaagccgc gctgtggggc tttgtccaac aggcgctggc
     8941 gacgttttgc gagaacccgg aaaatctcca tcagcctgca gtgcgaaaag tgctggggga
     9001 taatttgcta atggcgatgg gggcgatgct ggaagacgcg caaccaatgg tgacggcgga
     9061 aagcatcagt catcagagtt accgtagatt actttcccgc gcccgtgaat atgtgctgga
     9121 aaatatgtct gagccggtga cggtgctgga cttgtgtaat caactgcatg tcagtcgccg
     9181 cacgctacaa aacgcgtttc acgctatttt aggcattggc ccaaacgcgt ggctgaaacg
     9241 cattcgcctg aacgccgtac gccgcgaact gataagcccg tggtcgcaaa gcacaacggt
     9301 aaaagacgcc gccatgcagt ggggattctg gcatctgggg caatttgcca cggattacca
     9361 gcagctgttt gccgagaagc cgtcgttgac gttgcatcag cggatgcggg aatgggggtg
     9421 agggagttac acccaatccc tgaccttaat aaactcactt aacgccgctt ctgggctgcc
     9481 atcttttggc tgataatcat actcccagcg taccagtggc ggcattgaca tcaggatcga
     9541 ctcggtgcgc ccgccagttt gcaggccaaa cagagtgccg cgatcccaga ccagattgaa
     9601 ctcgacataa cgaccgcgac ggtagagctg aaaattgcgc tcgcgctcgc cgtaggccat
     9661 cgctttacgt cgctctacaa ttggtaaata agcgtcggtg tagcctttgc ctaccgcctg
     9721 cataaaggca aaacagtggt cgaaatctgg cgtgttcaga tcatcaaaga acagcccgcc
     9781 aataccgcgc tgttcgttgc gatgtttgag gtagaagtaa tcgtcgcacc actttttgta
     9841 acggggataa acgtcttcac caaatggcag gcacaggtca cgggcggtgc ggtgccagtg
     9901 aatggcgtct tcttcaaaac catagaaagg ggttaaatcg aagccgccgc caaaccacca
     9961 gacgggctct gcacccggtt tttcggcaat aaaaaaacgc acattcgcgt ggctggtggg
    10021 aacatacggg ttatgcggat gcactaccag tgaaacgccc atcgcctcga aactgcgccc
    10081 ggcaagttcc gggcgatgag cggtggcgga agctggcatc gcctcgccgt ggacgtgaga
    10141 aaagttgacg cctgcctgtt cgaaaacacc accattacgc aacacccgac tacgcccgcc
    10201 gccgccagct tcgcgctgcc aactatcttc gacaaattct gcgccatcga cggcggtcag
    10261 ctgctgacaa atcgtatcct gaaggttgag cagaaactgt ttaacctggt gtgcgtcggg
    10321 tttcataact tatcgctttt tcgaatgtgc tttctggttg tcgaaccagt ggaaataact
    10381 gatcacgcct tcagcaatcg ctgtggcgat tttctgacga aacgccgccg tgcctaacag
    10441 ccgctcttct tccgggttgg tgataaacga ggtttccacc agcaccgaag gaaccgacgg
    10501 cgatttcaac accacaaacg cggcttgttc ggtgttgcgg ctgtgcagtt tatgcaccgg
    10561 cttaatcttt ttcagaatat gcgagccgag cgtcaggctg tttttaatag tatcggtttg
    10621 caccagatca aacagcactt gctgcaatag gtgatccttg tcagtcgcct ttttaccggc
    10681 aacttcatcg gcgcggtttt cgcgttcaga caggtatttc gccattgcgc tacttgcccc
    10741 acggttagag agggcaaata ccgaagcacc ggcagctttc gggttggtaa agccatcggc
    10801 atgaattgac ataaacagat ctgcgccatg tttatgggcg atttcaacgc gatcgtaaag
    10861 tgggataaac gtatcgccag aacgcgttaa acgcgcatca atcccatgat tacgcaaaat
    10921 ggaacggacg tttttagcaa tcgccagcac cacatgtttt tctttcgaac cgttgcgtcc
    10981 gatcgctccg gtatcaattc cgccgtggcc tggatcgaga acaacgacac gtttgccgcc
    11041 agattttttg gctttcggct tgctgtgtcc gttgctggtt tttaaaggtt cgtctttggc
    11101 gatggcttgc gacattcctg acaacgtcag ggcagccaaa ccggctttca gcacctggcg
    11161 gcgcgaagtg agtgttttta gtggtttaaa agtgctcata cggcctgagt tgtaataata
    11221 aagttccaga tgttatatcg tatcgcgtaa tccgttacga cggtttgatg ttgagaattg
    11281 ttttactttt catttcaata gttgacaact ccccattatg ccactttttc aaccgatttt
    11341 cgtcagatat tggctaattc gaccgttcac gccataatca gccttttaac ccgcaaaaag
    11401 gtaacgaata ccatggagat acgcgtattt cgccaggaag atttcgaaga ggtcatcacc
    11461 ctttgggagc gttgcgactt gctgcgtccg tggaacgatc cggaaatgga catcgagcgt
    11521 aagatgaacc atgacgtcag tttgtttttg gtcgctgagg taaacggtga agtggtcgga
    11581 acggtgatgg gcggttatga cgggcatcgc gggtctgctt attatcttgg cgtgcatcca
    11641 gagtttcgtg ggcgtgggat tgccaatgcg ttgcttaatc ggctggagaa aaagctgatt
    11701 gctcgtggct gcccgaaaat tcagatcaat gtgccggaag ataacgacat ggtgctcgga
    11761 atgtatgaac gcctgggata tgaacacgcc gacgtgctga gtctgggtaa gcgtttgatt
    11821 gaagatgaag agtactgagt tccatcctgt ccattatgat gcgcacggac gcctgcgttt
    11881 acccttgctc ttctggcttg tgctattgct tcaggcgcga acctgggtgc tgtttgtcat
    11941 tgccggtgcg tcgcgcgagc agggtactgc gctgctaaat ctgttttatc ccgatcacga
    12001 taatttctgg ctggggctga ttccaggcat tccggcggtg ctggcgtttt tgttgagcgg
    12061 tcggcgggct acatttcctc gcacttggca tgtgctctat tttctgttgc tgttggcgca
    12121 ggtggtttta ctttgctggc aaccgtggct gtggctgaac ggtgaatccg ttagcggtat
    12181 cggtctggcg ctggtagtgg cggatattgt ggcgttaatc tggctgctga ccaatcgacg
    12241 tttacgcgct tgttttaatg aagaaaaaga ataacggcac tttttggtga atttgcactc
    12301 caagcaacgt tattgaataa ccaaaggaag tgacatgaaa tcgctgcgtt taatgttatg
    12361 cgctatgccg ttgatgctga ccggctgttc cacgatgtcg tcagttaact ggtcggccgc
    12421 taacccgtgg aactggtttg ggtcgtccac caaagtgagc gagcagggcg ttggtgaatt
    12481 aacggcgtcc acaccactgc aagaacaagc cattgccgat gcgcttgatg gcgattatcg
    12541 cctgcgcagc ggaatgaaaa ccgcgaacgg caacgtggtg cgcttttttg aagtgatgaa
    12601 gggcgacaac gtggcgatgg tgattaacgg cgatcaaggc acgatcagtc gcattgacgt
    12661 gctggatagc gatattcctg ctgacaccgg tgttaaaatc ggtacaccgt ttagcgacct
    12721 ttacagcaaa gcatttggca attgccaaaa agctgatggt gatgataatc gtgccgtcga
    12781 atgtaaagcc gaaggcagtc aacatattag ctaccagttc agcggggaat ggagtggtcc
    12841 tgaagggtta atgccttcgg acgataccct gaaaaactgg aaagtcagta aaattatctg
    12901 gcggcgttaa tttgcgtctg aacaaaccgc cgccgcgaaa aaacacgtaa aatagcgctc
    12961 aacaatgcca cggattgcgt ggcattatta ttttcaggag gaacaatgtc tcaggttcag
    13021 agtggcattt tgccagaaca ttgccgcgcg gcgatttgga tcgaagccaa cgtgaaaggg
    13081 gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg acaaactggc aacttttgaa
    13141 gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct ttggtaacaa cacctggcgc
    13201 gctctgagcg gcggcgttgg ggcagaagag ctgaaagatt ttccgggcta cggtaaaggc
    13261 cttgcgccga ccacccagtt cgatgtgttg atccacattc tttctctgcg tcacgacgta
    13321 aacttctctg tcgcccaggc ggcgatggaa gcctttggtg actgcattga agtgaaagaa
    13381 gagatccacg gcttccgttg ggttgaagag cgtgacctga gcggctttgt tgacggtacg
    13441 gaaaacccgg cgggtgaaga gacgcgtcgc gaagtggcgg ttatcaaaga cggcgtggat
    13501 gcgggcggca gctatgtgtt tgtccagcgt tgggaacaca acctgaagca gctcaaccgg
    13561 atgagcgttc acgatcagga gatgatgatc gggcgcacca aagaggccaa cgaagagatc
    13621 gacggcgacg aacgtccgga aacctctcac ctcacccgcg ttgatctgaa agaagatggc
    13681 aaagggctga agattgttcg ccagagcctg ccgtacggca ctgccagtgg cactcacggt
    13741 ctgtacttct gcgcctactg cgcgcgtctg cataacattg agcagcaact gctgagcatg
    13801 tttggcgata ccgatggtaa gcgtgatgcg atgttgcgtt tcaccaaacc ggtaaccggc
    13861 ggctattatt tcgcaccgtc gctggacaag ttgatggcgc tgtaagcgtt tctgattcat
    13921 tgattaaagg ccaggcagta acattactga ctggcctttt tattactttt gctttaacgc
    13981 cgcatacacc tgatcaacta cccaaccata cgttgcaatc ggcagctgac cgctttcgaa
    14041 catattggga ttcttttgcg gatcggcaac cggcgaatgt ggcttgttgc ttaacatcac
    14101 aatcgccata tgattcaccg gatcgataac ggtcaccgtt ccggtccagc cagtgtgacc
    14161 gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc atggtggcat taccattcac
    14221 gcgccagccg aggccaaaag tggcatcttc cttagagctg gtggtgaaca tcttcaccgt
    14281 ttccgcactg aacagctgca catcgccata gcccccgccg ttcagcatcg tttgcattaa
    14341 caccgcaata tcgccggtat tggaaaacaa acctgcgtgc ccggaaacgc cgcccatcga
    14401 ataaaaggct ttttcatcgt gcacctgacc ccagagagtg gaggtgcgga tattcgggaa
    14461 atggatcacg ccatcgcggg tattgccgtt caattccgtg gcggcaattt gctgcggttt
    14521 aaagcctttc agcagcgggt taaataccgt atgtgtcagg ccgagcgggc gataaatcga
    14581 ctcttcaaca tagcggtcaa gcggctgacc ggtgaccgac tcgacgataa atccaagcag
    14641 catataatcg acatcactgt aaatatgctt gctgccgggc tgatactcca gcggcgtgcg
    14701 cttgatcatc tccagcgtct ggcctttatc ctgggaatat aacgcgcccg cgaccgcttt
    14761 attcggatat tgtgggtccg ccgggaaacc gccgctgtga tgcagcaggt cagaaatccg
    14821 cagcgtgttt ttgcctttga tggtgtcatt tggactatcg gcaaaccccg gaatatattt
    14881 cgcaatccga tcgtcaggat gcagtttgcc ttcgctcatc agcttttgca gggcgaagtt
    14941 cgtggcgtac attttggtgt ttgaggccag gtcatacagc gtcccggtgg tggctttgac
    15001 aggctgttcc atcaacacgc tgccatcgta ctttttcgcc gctccccagg ctttgcgata
    15061 cacaatctga ttatctttaa tgatcagcag gtttacgccg ggataaccgg cgtccacttg
    15121 ctggctaatc cagcgatcca tctggttaag ccgttcgacg ttgaaccctg ctttctctgg
    15181 cgaactttct gtcagaacag ggtattttgc ggcgctgacg ctacaactaa cagccagcag
    15241 agaaagatag agcattgtcc gtttcatgat atcagtccag attgacgtta cggcagccaa
    15301 agagcgtggt gaaaataaac ccgcaaaccc atgccaccag aatcccgcca gcataaaccg
    15361 ccattgccgg aaggatgcct tgtgcggaag tcatcagcgg cagcgccacc agaccagacg
    15421 gcccaaaggc gctgtttaag cccatcggca gaccccacca ggctatcagg ccgataaaca
    15481 aaccgcccgc tgcgccgcct aaacaggcgg taataaacgg tttcatgcgg ggcagggtga
    15541 cgccgtaaat caacggttca ccaacgccca gcagaccggg aataatcgcc ccgcgtacct
    15601 gactgcgtaa tgcactgtgc ggttgcgccc gccagtagag tgccagcgcc gcgcccacct
    15661 ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt gaatccctgg ctgtccatta
    15721 acgcgaggta aacaggaata aagccctgat gcacgccaaa taccacggcg atcaggaaca
    15781 gcccggctaa aaccgcacaa ccgaacggat tactgttcag gtgcataaac agccatgaca
    15841 taccttcgaa taaccagcca cccagcggca taatgatcag gtacgcaagc gtagcggtga
    15901 tcagcagggt gattaacgag gtcagcagca tgtcgagatc gtccggcata aagcggcgca
    15961 ccatgccttc aatgcgggcg caggcccacg cggcaatcag cacgccgata atattgccgc
    16021 gcggatcgat gggcagacca aagaaatcgt gaaaaccggc gtagtaaccg gtggttgcag
    16081 cggggttata accgagcaaa aagagtgcgg caataattgc gccattgacg cccgtgccgc
    16141 caaatgcctg ggcggcgtta tagcccacca gaatcaccag gaaagtgaac aaacctttgc
    16201 tgaacacctt cataaaattc agcgcgtcgg gtagcgtgcc ctgagcatct gccggaacgt
    16261 gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag accggcggca ataaaaccgg
    16321 ggatcagcgg cgtgaagatg gtggcgaatt tggcgagaaa ttgttgcacg ccggaggttt
    16381 gtttggcttt taactgacgt ttgttctggg cggcgatttc tgcggcatcc tgtaccggtg
    16441 cctcgcccag cagctcgctc atcgcttttg cggcacgatg cgctttacca ggcccaaaaa
    16501 cgacctgcac ctggtcgctg gtcaaaatga cgcccttcac gccttccagc gttttgatat
    16561 tgggatcaac cagtgaactg tcatgtacac ccagacgcag gcgcgtcata cagttaccac
    16621 aactggcgat atttcccggt ccgccgacac gggtaagaat ggtgttcaga agttcactgc
    16681 tgatctcttt ggccatgggt tattccttgt ctaaaacctg acgaataaag ccgccgtgtt
    16741 gatccaggcg ttttttagct tctgcggcat cgagattttt cagcaccatc acaatggccg
    16801 ttttacagtt gcgctcgcag gcaattaacg ccgcttccgc ttgttcggca ttacatccgg
    16861 tggcgttttt aacgatattg acctggcgca catgcagttt ttcgttggtg gcgaccacat
    16921 cgaccatcag gttgccgaac actttgccag atttaatcat cagcccggtg gaaagcatat
    16981 tgagcaccag tttctgcgct gtgcctgctt tcattcgtga agaaccagtg accacttcgg
    17041 cacctacaat cggtgtaatg gcaaactcgg cggtggtgga aacggcactc cccggattac
    17101 aggaaattcc cactgtgcgg cagccgagct ggcgtgcgta ttccagtccg gcaatcacat
    17161 acggcgtgcg accgctggca gcaatgccaa ccaccacatc ctgtgccgtt aaattaatat
    17221 ttttcagatc gttaacacca ccttcccggc tatcttcagc gccttccacc gcgtgctgaa
    17281 tggcatattc gcctccagca atcaaaccaa ccaccagacc cggtttcacg ccgtaggtgg
    17341 gcggacattc gctggcatcc agaatcccca gacgaccgga tgttcccgca ccgagataaa
    17401 tcagacgacc gccgccgctg acctgggcgt ggataacatc gatcgccgcg gcgatatccg
    17461 gcaggacgcg ctcaacggca agcggtacgg ttttatcttc atcgttgata atccggcaca
    17521 tttccagcgt cgatacgcgg tcaatttcag ccgaggcggt gttcgagcct tcagtaatca
    17581 tcttttctag ttgcatcggt ggtgacctta ctatttacga tcaaataatt gcagatgtat
    17641 ggaatcatat atcccgaatt gacagactta aaggaatcat tgagtctaca ctcgcagagt
    17701 gtgaagagcg tcataagaac ttaagcggaa tttatgctgc cagcccagga gagtggaaga
    17761 tgttgtacct gacgaaaata agcaatgcgg ggagtgaatt caccgaaaac gagcaaaaaa
    17821 tcgctgactt tctgcaggcc aacgtgagtg agctgcaatc cgtctcttcg cgcaagatgg
    17881 cgaaacagct cggcatcagc cagtcgagta ttgtgaagtt tgcccagaag ctcggcgcac
    17941 aaggatttac tgaattgcgt atggcgttaa ttggcgaata cagtgccagc cgggagaaaa
    18001 cgaatgccac ggcgctgcac ctgcacagtt cgattaccag tgatgattcg ctggaagtga
    18061 ttgcccgcaa actgaatcgc gaaaaagagc tggcgctgga gcaaacctgc gcgttgttcg
    18121 actacgcgcg gctgcaaaaa atcattgatg tcatcagtaa agcgcaattt attcagataa
    18181 cgggcctggg cggatcggcg ctggtggggc gcgatctgtc gttcaaactg atgaaaattg
    18241 gatatcgcgt ggcctgcgaa gccgacacgc acgtacaagc caccgtttcc caggcgttga
    18301 aaaaaggcga tgtacaaatc gctatctctt acagcggcag taaaaaagag attgtcttgt
    18361 gcgcagaagc ggcacgaaag cagggggcaa cggtgattgc cattacttct ctggctgact
    18421 ctccgctgcg gcggctggcg catttcacgc tcgataccgt ttccggtgaa accgaatggc
    18481 gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt taccgattta ctgtttgttg
    18541 gtcttgtgca actcaatgat gtcgagtcat tgaaaatgat tcagcgcagc agtgagctaa
    18601 cacaacgcct gaaatgatgc ataaagcagc gactggattg agattttcct gaattagtga
    18661 gctgatccgc agcaatattt tgtttatcct gtattttcag agggaatgga gtgtaacgct
    18721 ctgtattaac aaggagagca ttaaaatggg taaactcacg ggcaagacag cactgattac
    18781 gggcgcattg cagggaattg gcgaaggaat tgccagaact tttgcgcgtc atggcgcaaa
    18841 cctaatcttg ctggatatct cccctgagat cgaaaagctg gcggacgaac tgtgtggtcg
    18901 tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac ccggcgtcgg tagccgcagc
    18961 tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc ctggtgaata acgcaggcgt
    19021 ttgtcgtctg ggcagtttcc tcgatatgag cgatgaagat cgcgatttcc atattgacat
    19081 caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg ccagagatga ttgcgcgcaa
    19141 agatggtcgc attgtgatga tgtcttcagt cactggtgat atggtggccg atccgggtga
    19201 aacggcgtac gccttaacga aagcggcgat tgttggcctg acaaaatcgc tggcggtgga
    19261 gtacgcgcaa tccggtattc gcgttaacgc catctgcccg gggtacgtcc gcacgccaat
    19321 ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca gagtcggtgc tgactgatat
    19381 ggcaaaagca atcccgctgc gtcgcctcgc cgatccgctg gaagtcggcg aactggcggc
    19441 cttcctcgca tcggatgaat ccagctattt aaccggtaca cagaatgtga ttgatggcgg
    19501 cagcacactg ccggagacgg ttagcgtcgg tatctgattc acctctgttt cctccctgca
    19561 tgtgtgggga ggatttcgtt ttaaacccgg ttcttattcc cttttcaact tccaaatcac
    19621 caaacggtat ataaaaccgt tactcctttc acgtccgtta taaatatgat ggctattaga
    19681 aagtcattaa atatataagg gtgcgcaatg gccgttaact tactgaaaaa gaactcactc
    19741 gcgctggtcg cttctctgct gctggcaggc catgtacagg caacggaact gctgaacagt
    19801 tcttatgacg tctcccgcga gctgtttgcc gccctgaacc cgccgtttga acagcaatgg
    19861 gccaaagata acggcggcga caaactgacg attaaacaat ctcatgccgg gtcatcaaaa
    19921 caggcgctgg cgattttaca gggcttaaaa gccgacgttg tcacttataa ccaggtgact
    19981 gacgtacaaa tcctgcatga caaaggcaag ctgatcccgg cggactggca gtcgcgcctg
    20041 ccgaataata gctcgccgtt ctactccacc atgggcttcc tggtgcgtaa gggcaacccg
    20101 aagaatatcc acgactggaa cgacctggtg cgctccgacg tgaagctgat tttcccgaac
    20161 ccgaaaacgt cgggtaacgc gcgttatacc tatctggcgg catggggcgc agcggataaa
    20221 gctgacggtg gcgataaagc caaaaccgaa cagtttatga cccagttcct gaaaaatgtt
    20281 gaagtgttcg ataccggcgg tcgtggcgca accaccactt ttgccgagcg tggcctgggc
    20341 gatgtgctga ttagctttga atcggaagtg aacaacatcc gtaaacagta tgaagcacag
    20401 ggctttgaag tggtgatccc gaaaaccaat attctggcgg aattcccggt ggcgtgggtc
    20461 gataaaaacg tgcaggccaa cggtacggaa aaagcagcaa aagcctacct gaactggctc
    20521 tacagcccgc aggcgcagac catcatcacc gactattact accgcgtaaa taacccggaa
    20581 gtcatggaca aactgaaaga taaattcccg cagaccgagc tgttccgcgt ggaagacaaa
    20641 tttggctcct ggccggaagt gatgaaaacc cacttcacca gcggcggcga gttagacaag
    20701 ctgttagcgg cggggcgtaa ctgatgtttg cagtttcatc aagacgcgtg ctgccgggct
    20761 ttaccttaag cctcggcacc agtctgctgt ttgtgtgcct gattttgctg ctgccgctct
    20821 ccgcgctggt gatgcagctg gctcagatga gctgggcgca gtactgggag gtgatcacca
    20881 acccacaagt ggtcgcggcc tacaaagtga cgctgctgtc ggcgtttgtg gcatcgattt
    20941 ttaacggcgt tttcggtctg ctgatggcgt ggattttaac acgttatcgc tttccgggcc
    21001 gcacgctgct tgatgcgctg atggatttac cctttgcgct gccaacggca gtcgccggtt
    21061 taacgctggc ctcgctcttt tccgtaaacg gtttttacgg tgaatggctg gcgaagtttg
    21121 atatcaaagt cacctatacc tggcttggta ttgcggtggc gatggccttt accagcattc
    21181 cgtttgtggt gcgtaccgtg cagccggtgc tggaagagtt aggcccggaa tatgaagaag
    21241 cggcggaaac gcttggtgca acgcgctggc agagtttctg caaagtggtg ctgccggaac
    21301 tttctccagc gctggtggcg ggcgtggcgt tgtcgtttac ccgtagtctt ggtgagtttg
    21361 gcgcggtgat atttatcgcc gggaacatcg cctggaagac ggaagtgacg tcgctgatga
    21421 tttttgtgcg cttgcaggag tttgattacc cggcggcgag cgcgattgct tcggtgatcc
    21481 tcgcggcatc cctgctgctg ctgttctcaa ttaacactct gcaaagtcgc tttggtcggc
    21541 gtgtggtagg tcattaatgg cggaagttac ccaattgaag cgttatgacg cgcgcccgat
    21601 taactggggc aaatggtttc taattggcat cgggatgctg gtttcagcgt tcatcctgct
    21661 ggtgccgatg atttacatct tcgtgcaggc attcagcaag gggctgatgc cggttttaca
    21721 gaatctggcc gatccggaca tgctgcacgc catctggctg acggtgatga tcgcgctgat
    21781 tgccgtaccg gtaaacctgg tgttcggcat tctgctggcc tggctggtga cgcgctttaa
    21841 cttccctgga cgccagttac tgctgacgct actggacatt ccgtttgccg tatcgccggt
    21901 ggttgccggt ctggtgtatc tgctgttcta cggctctaac ggcccgctgg gcggctggct
    21961 ggatgaacat aatctgcaaa ttatgttctc ctggcccgga atggtgctgg tcaccatctt
    22021 tgtgacgtgt ccgtttgtgg tgcgcgagct ggtgccggtg atgttaagcc agggcagcca
    22081 ggaagacgaa gcggcgattt tgcttggcgc gtctggctgg cagatgtttc gccgcgtcac
    22141 attgccgaac attcgctggg cgctgcttta tggcgtggtg ttgaccaacg cccgcgcaat
    22201 tggcgagttt ggcgcggtgt cggtggtttc cggctcgatt cgcggcgaaa ccctgtcgct
    22261 gccgttacag attgaattgc tggagcagga ctacaacacc gtcggctcct ttaccgctgc
    22321 ggcactgtta acgctgatgg cgattatcac cctgttttta aaaagtatgt tgcagtggcg
    22381 cctggagaat caggataaac gcgcacagca ggaggaacat catgagcatt gagattgcca
    22441 atattaagaa gtcgtttggt cgcacccagg tgctgaacga tatctcactg gatattcctt
    22501 caggtcagat ggtcgcgttg ctggggccgt ccggttccgg gaaaaccacg ctgctgcgca
    22561 ttatcgccgg gctggagcat caaaccagcg ggcatattcg cttccacggc accgacgtca
    22621 gccgcctgca cgcgcgcgat cgcaaggtcg gtttcgtgtt ccagcattac gcgctgttcc
    22681 gtcatatgac ggtgttcgac aatatcgctt ttggcctgac ggtgctgcca cgtcgcgagc
    22741 gcccgaatgc cgcagccatc aaagcgaaag tgacaaaatt gctggagatg gtgcagcttg
    22801 ctcatctggc agatcgttat ccggcacagc tttccggcgg ccagaaacag cgcgtggcgc
    22861 tggcgcgcgc gctggctgta gaaccgcaaa ttctgctgct tgatgaaccg tttggcgcgc
    22921 tggatgcgca ggtgcgtaaa gagctgcgtc gctggctgcg tcaactgcat gaagagctga
    22981 aattcaccag cgtgttcgtg acccacgacc aggaagaggc gaccgaagta gccgatcgtg
    23041 tagtggtgat gagtcagggg aatattgaac aggctgatgc gccgaatcag gtatggcgcg
    23101 aaccagcgac ccgttttgta ctcgaattta tgggcgaagt gaaccgcctg cagggaacca
    23161 ttcgcggcgg gcagttccat gttggcgcgc atcgctggcc gctgggctac acacctgcgt
    23221 atcagggtcc ggtggatctc ttcctgcgcc cgtgggaagt ggatatcagc cgccgtacca
    23281 gtctcgattc gccgctgccg gtacaggtac tggaagccag cccgaaaggt cactacaccc
    23341 aattagtggt gcagccgctg gggtggtaca acgaaccgct gacggtcgtg atgcatggcg
    23401 acgatgcccc gcagcgtggc gagcgtttat tcgttggtct gcaacatgcg cggctgtata
    23461 acggcgacga gcgtatcgaa acccgagatg aggaacttgc tctcgcacaa agcgcctgat
    23521 aggttgagtg aatgttaaac gcccggaggc gcttcccgcg atccgggctt tttaatggca
    23581 aggtttgtaa cctgtagacc tgataagacg cgcaagcgtc gcatcaggca acaccacgta
    23641 tggatagaga tcgtgagtac attagaacaa acaataggca atacgcctct ggtgaagttg
    23701 cagcgaatgg ggccggataa cggcagtgaa gtgtggttaa aactggaagg caataacccg
    23761 gcaggttcgg tgaaagatcg tgcggcactt tcgatgatcg tcgaggcgga aaagcgcggg
    23821 gaaattaaac cgggtgatgt cttaatcgaa gccaccagtg gtaacaccgg cattgcgctg
    23881 gcaatgattg ccgcgctgaa aggctatcgc atgaaattgc tgatgcccga caacatgagc
    23941 caggaacgcc gtgcggcgat gcgtgcttat ggtgcggaac tgattcttgt caccaaagag
    24001 cagggcatgg aaggtgcgcg cgatctggcg ctggagatgg cgaatcgtgg cgaaggaaag
    24061 ctgctcgatc agtttaataa tcccgataac ccttacgccc attacaccac taccgggccg
    24121 gaaatctggc agcaaaccgg cgggcgcatt acccattttg tctccagcat gggaacaacc
    24181 ggcactatca ccggcgtttc acgctttatg cgcgaacaat ccaaaccagt aacgattgtt
    24241 ggcctgcagc cggaagaagg cagcagtatt cccggcattc gccgctggcc tgcggaatat
    24301 ctgccgggga ttttcaacgc ttctctggtg gatgaggtgc tggatattca tcagcgcgat
    24361 gcagaaaaca ccatgcgcga actggcagtg cgggaaggaa tattctgtgg cgtcagctcc
    24421 ggcggcgcgg ttgccggagc actgcgggtg gcaaaagcta accctggtgc ggtggtggta
    24481 gcgatcatct gcgatcgtgg cgatcgctac ctttccaccg gggtgtttgg ggaagagcat
    24541 tttagccagg gggcggggat ttaagattat tgcgcaggca ccgttttatc cgtctgcgca
    24601 attagcgcat gtaaaaaagg ctgtgcgttc tcatcgctct taccggggat cttcacaata
    24661 tacggcttcc cggtaatcga tgacgacgaa gcaaccttat caataaactg ctcggcagtg
    24721 tcaatgcggt tacgggtatt gcccagcttc agacgcagat gagaaaccgc ttcatcgcag
    24781 gtatgttcat caccgttgcg cacaaatatc aaatcctttt tctgtgctaa tccctccagc
    24841 atggcgttga tgcgggcttc ttcatgggca gttaacttcg cgtaagcggg gagtgtcatt
    24901 agtagtgtaa tgaccagaca aatgattttc ttcattgaat taccttatcc ttgttttgtg
    24961 atagccgatt tgactgccag aatcaggtaa agtttcggtt ggaatacgat gaggatcatc
    25021 ggcaaggaga aaaaattaaa tgagtagttt gttgttgttt aacgataaga gcagggcgct
    25081 gcaggcggat atcgtcgccg tgcagtcgca ggtggtttac ggcagcgtgg gcaacagcat
    25141 tgccgtgcct gctatcaaac agaacggtct gaatgttttt gccgtgccga cggtattgct
    25201 gagcaatacg ccgcattatg acactttcta cggtggtgcg attccggatg aatggtttag
    25261 cggttatttg cgtgcgcttc aggagcgtga tgcgctgcgc cagctgcgcg ctgtaaccac
    25321 gggctatatg ggaacggcat cgcaaatcaa aatccttgcc gagtggctga ctgcgctacg
    25381 caaagaccat cctgacctat tgatcatggt cgatccggtg attggcgata ttgatagcgg
    25441 aatttatgtc aaacctgacc ttcccgaggc gtatcgacaa tatttactgc cgctggcgca
    25501 gggaattacc cccaatatct ttgagctgga aatcctgacc ggtaaaaatt gccgcgatct
    25561 cgacagtgcc attgctgccg caaaaagtct gctttcagac acattaaaat gggtggtgat
    25621 taccagcgcc tccggtaatg aagaaaatca ggagatgcag gtggtggtgg tcagtgccga
    25681 cagcgtgaat gtcatttccc attcacgggt aaaaaccgac ctgaaaggga ctggcgacct
    25741 gttttgtgct cagcttatca gtggtttgct gaaagggaag gcgttaaacg atgcggtgca
    25801 ccgtgcgggg ttgcgcgtac tggaagtgat gcgctacacc cagcagcatg agagcgatga
    25861 attgattttg ccgccgctgg cggaagcata aaaaatggcg ccgatgggcg ccatttttca
    25921 ctgcggcaag aattacttct tgatgcggat aaccggggtt tcacccacgg ttacgctacc
    25981 ggacagtttg atcagttctt tgatttcgtc catgttggag ataacaaccg gagtcagggt
    26041 agacttggct ttctcttcca gtagcggcag atcaaattca atgacagtat cgccaacttt
    26101 cacgcgctga ccttcttcag caatacgctt gaagccttcg cctttcagtt caacggtgtc
    26161 gataccgaag tggacgaaca gttcaacgcc gctatcagat tcgatagaga acgcgtgatt
    26221 ggtttcaaag attttaccaa tggtaccgtc tacaggcgcg accattttgt tacccgttgg
    26281 tttgatagca ataccatcac caacgatttt ttccgcaaaa acgacatccg gcacgtcttc
    26341 gatattgacg atctcgccag agagcggagc aatgatctca atagttccgg tatccttctt
    26401 gtcgtcggaa accagagatt tcagtttatc gaacaaaccc atgatcttct cctaagcagt
    26461 aaattgggcc gcatctcgtg gattagcaga ttgttttttc ttcaatgaac ttgttaacca
    26521 gcgtcattaa ctcgtccgtt gtcggttgag caagagcctg ctctgctaac accttcgcat
    26581 cttcgaagtt cgtgttacgg ataatcttct taatgcgcgg gatagaaatg gcgctcatag
    26641 agaattcgtc cagacccatc cccagcaaca gaagtgtagc acgttcatcg ccagcaagct
    26701 caccacacat gccagtccat ttgccttcag catgagaagc atcaataact tgcttgatca
    26761 agttcagcac ggacggtgac attggttggt aaaggtgtga aatcatatca ttaccacggt
    26821 caactgccag agtgtactgc gttaaatcat tggtgccgat actaaagaaa tcaacttctt
    26881 tggctaaatg acgtgcaatt gttgcggcag ccggtgtttc caccattacg ccgatttcaa
    26941 ttgactcgtc aaacgcttta ccttcgtcgc gcagttcctg tttgtagatt tcgatctctt
    27001 tgcgcagtgc acgcacttct tcaacagaga tgatcatcgg gaacataatg cgcaatttac
    27061 cgaaagccga ggcacgcagg atagcgcgga gctgatcgcg caggatctct ttacgatcca
    27121 tcgcgatacg gatagcgcgc cagccgagga acgggttctc ttctttcggg aagttcatgt
    27181 atggcagctc tttgtcgccg ccgatgtcca tggtacgaac gataaccgcc tgcgagccac
    27241 acgcttcagc cactgctttg taagcagcaa actgttcttc ttcagtgggc agcgcgtcgc
    27301 ggtccatgaa caggaactca gtacgataca gaccaacgcc ttcagcgccg ttacgctctg
    27361 caccttcaac gtcacgaacc gtaccaatgt tagcgcacac ttctacctgg tgaccgtcca
    27421 gcgtaatagc tggcagatct ttcagtttag caagctctgc tttttcagaa gccacttgct
    27481 cctgaacagc gcgcatttta tcaataactt cgttggttgg attgacgtaa acctgattat
    27541 ttacggcatc cagaatcaga tagtcgtcat ttttcacctg agaggtgacg ctaccggtac
    27601 ccacgatagc aggcagttcc agagaacgcg ccatgataga ggtgtgggaa gtacggccac
    27661 ccgcgtcggt gatgaaaccc agcaccttct tcaggttcag ctgtgcggtt tcggacggcg
    27721 tcaggtcagc ggcaaccaga atgacttcat cctgaatggc gctcaggtcg ataatcttca
    27781 ggcccaggat gttgcgcagc aggcgcttac cgatatcacg tacgtcagcc gcacgttctt
    27841 tcaggtattc atcatccagc tcttccaggg cagaagcctg accttcgata acttcatgag
    27901 cagctgcgtc agctgtcatg tgcttatctt taatcagggc tatgatttcc tgctccagct
    27961 cctcatcttc gagcagcata atatgccctt caaagatggc ttctttttct tcaccgaacg
    28021 tttcaccagc tttcgttttg atcgtttcca gctgggctga tgccttggca cgaccgctca
    28081 gaaaacgttc aacttcctga tcaacctggt cggcagaaat ttttttccgg tcaatgacaa
    28141 tttcgtcttc tttcagaagc agagctttac cgaaagcgat acccggggat gctaaaatgc
    28201 ctgaaatcat aaccctacct tacttgtgac tgatttttaa aagaacccgg gaaattactc
    28261 gagttccgcc atcagtttaa ccagatgttc aaccgctttc tgctcgtctt cgccttctgc
    28321 ggagatagtc acaacggtac cttgagtcag gcccagagtc tgcagtttaa acaggctttt
    28381 cgcgctggcg cttttgccgt tggaagtcac agtaatttca gaagtgaagc ccttagcttc
    28441 ttttacaaac tgggcagcag ggcgggtgtg cagaccgttc ggagcggtaa tggtaacttc
    28501 ttgctggaac attgtatttc cccaacttat aggtttagtg ttgtggaact aaagtctagc
    28561 ctggcggttc gactttagcc tgtattgtta gcgccagttt ttaacagacg cgacgcacga
    28621 agagcgcaat acagtccatt gctggcgggt gttgaccgct gacgtttcgt acgtcattaa
    28681 acattatgcc gctaaaggaa gaattgaacc aaatcataaa atcgattcag ctttggcttt
    28741 tcctgtaacg attaatttcg cgcatcaaaa taattagtct ggttaaatac cagttccgac
    28801 aggctgaatc aatgccagga ggggcgaaag tttgaagcag gccacaaaaa agcacctaaa
    28861 aaggtgcttt tttacgcatt tttaacaagc tggcattact gttgcagttc tttctcagtg
    28921 aagagatcgg caaacaatgc ggtgcttaaa taacgctcac ccgatgacgg tagaataacc
    28981 acaatattct tgttggtaaa gctttcatct tcttgtagtt tcaacgccgc agcaacagct
    29041 gctccagaag agatacctgc aagaatacct tcttcttcca tcagacgacg cgcggtagaa
    29101 atcgcttctt cattggtgat gccaatgact ttatcgacca gcttgagatc gaggttagct
    29161 gggataaaac cagcgccaat accctgaatt ttatgcgggc caggtttaat ctcttcacca
    29221 gccagtgcct gggcgataac tggagaatcg gttggctcaa cggcgacaga gataagatcg
    29281 gtcttgcctt tggtgccttt aatgtagcgg ctgacgccag tcagcgtacc gccagtccca
    29341 acgccagcaa taaatacatc aacctgaccg tcggtatctt cccagatctc cggaccggtg
    29401 gttttttcgt gaatttcagg gtttgccgga ttgctgaatt gttgcagcag caggtatttc
    29461 tctggattgc tggcgacaat ttcttctgct ttttggattg cgcctttcat gcctttagca
    29521 ccttccgtca gcaccaggtt tgcacctaac gctttcagca gcttgcggcg ttcaatactc
    29581 atggtttctg gcatggtcag ggtgagtttg taaccgcgag cggcagctac ataggccagt
    29641 gcaatcccgg tattaccgct ggtcggttca accagttcaa cgcctggttt cagcacgccg
    29701 cgcttttcgg catcccaaat catgttggca ccgatacggc acttaacgct gaagctgggg
    29761 ttacgagatt ccaccttcgc cagaatgcgt ccgttaccga tgcgattcag gcgaaccagc
    29821 ggcgtgtgac cgatagtcag cgagttatct tcaaaaatct tactcatggc ctgtccttaa
    29881 ctgtatgaaa ttgggataca acaggtagca tacccgctca gagaatatgc ggaagtaagg
    29941 atttagcata tctatataca gaagggaaat aatgacatgc aagatggaat aaggggcggc
    30001 ataagccacc cctgtttcac acaaacggtt tacaaaataa ccggtagatt gttaccgcca
    30061 catcgcgtgt ttatcgcgat agcaatcgac ccacatcgcc gtcgcgccac aaacggcaac
    30121 gggcatgatg aacagattaa gcagcgggat catcgtaaac aggctggtta aagcaccaaa
    30181 ctgcatattg gtgattttgc gtgtgcgcag ggcggtgcgc atctctttaa acggcacttt
    30241 gtggttatcg aaggggtaat cgcaatactg gatggctaac atccaggcgc taaacaggaa
    30301 ccacagtacc ggcgcgacgg tttgcccaat accagggatt aagtaaagaa ttagcaatac
    30361 aattgcgcgc ggcagatacc aggcaaattt ttgccattcg cgtttcatga ttcgcggcac
    30421 atctttcatg ataccgaaaa tcccggtatc tggcggtgta gcgccagtca atcgtgcttc
    30481 cagttgttca gccaataaac cgttaaacgg agcggcaatc cagttagcaa tcgtggagaa
    30541 gaaatagcca aacactaaca gcacagagat gacagccaga ggccacaaca gataactcag
    30601 ccattgtagc cagtccggaa cgtaactcat gagagtcggg atccagacat cgagctgtgt
    30661 aaagagccac cagaatgcgc cccccatcaa caaaatattg accagcagcg gtaaaataac
    30721 gaaacgccga atcccaggtt gcgagacgag cttccagcct tgcgcaaaat agtaaaaacc
    30781 gctgcgtggg gcagatgtga atgatgaaac cataatcagg atgagctcct tttgaccaat
    30841 cccaggaaaa ttctgcgtat tttaccgggt aattgcgcaa tggacagtta ggatatgttc
    30901 gaaaaaacag caaaaagcac gatttcatct atctttgtgc tgtgaaagtt aatagtgcac
    30961 ttgcacttga ggtaatcggc aaatactctt agtgagtaaa tgtttgccgt ggtggcaagg
    31021 tgttagaaca acagagaata taatgatgca ggatttgcgt ctgatattaa tcattgttgg
    31081 cgcgatcgcc ataatcgctt tactggtaca tggtttctgg accagccgta aagaacgatc
    31141 ttctatgttc cgcgatcggc cattaaaacg aatgaagtca aaacgtgacg acgattctta
    31201 tgacgaggat gtcgaagatg atgagggcgt tggtgaggtt cgtgttcacc gcgtgaatca
    31261 tgccccggct aacgctcagg agcatgaggc tgctcgtccg tcgccgcaac accagtacca
    31321 accgccttat gcgtctgcgc agccgcgtca accggtccag cagccgcctg aagcgcaggt
    31381 accgccgcaa catgctccgc gtccagcgca gccggtgcag cagcctgcct atcagccgca
    31441 gcctgaacag ccgttgcagc agccagtttc gccacaggtc gcgccagcgc cgcagcctgt
    31501 gcattcagca ccgcaaccgg cacaacaggc tttccagcct gcagaacccg tagcggcacc
    31561 acagcctgag cctgtagcgg aaccggctcc agttatggat aaaccgaagc gcaaagaagc
    31621 ggtgattatc atgaacgtcg cggcgcatca cggtagcgag ctaaacggtg aactgcttct
    31681 taacagcatt caacaagcgg gcttcatttt tggcgatatg aatatttacc atcgtcatct
    31741 tagcccggat ggcagcggcc cggcgttatt cagcctggcg aatatggtga aaccgggaac
    31801 ctttgatcct gaaatgaagg atttcactac tccgggtgtc accatcttta tgcaggtacc
    31861 gtcttacggt gacgagctgc agaacttcaa gctgatgctg caatctgcgc agcatattgc
    31921 cgatgaagtg ggcggtgtcg tgcttgacga tcagcgccgt atgatgactc cgcagaaatt
    31981 gcgcgagtac caggacatca tccgcgaagt caaagacgcc aacgcctgat acacttaagg
    32041 caaattaact cctcttcgaa cccccgcttg tcgggggttt ttagcattga tggtgcgata
    32101 tggaatcaat cgaacaacaa ctgacagaac tgcgaacgac gcttcgccat catgaatatc
    32161 tttatcatgt gatggatgcg ccggaaattc ccgacgctga atacgacagg ctgatgcgcg
    32221 aactgcgcga gctggaaacc aaacatccag aactgattac gcctgattcg cctacccaac
    32281 gtgtaggcgc tgcgccgctg gcggctttca gccagatacg ccatgaagta ccaatgctgt
    32341 cactggataa cgtttttgat gaagaaagct ttcttgcttt caacaaacgt gtgcaggacc
    32401 gtctgaaaaa caacgagaaa gtcacctggt gctgtgagct gaagctggat ggccttgccg
    32461 tcagtattct gtatgaaaat ggcgttttag tcagtgccgc gacccgtggc gatggcacca
    32521 ccggggaaga tatcacgtct aatgtgcgta ctattcgcgc tattccgctg aagctgcacg
    32581 gagagaatat cccggcgcgt ctggaagtgc gtggtgaagt gttcctgccg caggcggggt
    32641 tcgaaaagat taacgaagat gcgcgacgca cgggcgggaa agtttttgct aacccacgta
    32701 atgcggcagc tggttcactg cgtcagcttg atccgcgtat tacagcgaag cgaccgctca
    32761 cttttttctg ctatggcgtt ggtgttctgg aaggtggcga gctgccggat actcatcttg
    32821 gccgtttact gcaatttaaa aagtgggggt tgccggtcag cgatcgggta acgctttgtg
    32881 aatcggcgga agaagtgctg gcgttctatc acaaagtgga agaagaccgc ccgacgctgg
    32941 gctttgatat cgacggcgtg gtgattaagg tcaactcact ggaacagcag gagcagcttg
    33001 gctttgtcgc gcgtgccccg cgctgggcgg tagcgtttaa attcccggcg caggagcaga
    33061 tgacctttgt gcgtgacgtc gagtttcagg ttgggcgtac tggcgcgatt acgcctgttg
    33121 cgcgtctgga acctgtccat gttgctggcg tgctggtgag taacgcaacc ttacacaatg
    33181 cggatgaaat cgaacgtctt ggtttacgca ttggcgataa agtggtgatt cgccgcgctg
    33241 gcgacgtgat cccgcaggtg gttaacgtcg tgctttctga acgcccggaa gatacccgtg
    33301 aggttgtatt cccgacgcat tgtccggtat gtggttctga cgttgagcgt gtggaaggtg
    33361 aagcggttgc ccgctgtacc ggtggcctga tttgcggtgc gcagcgtaaa gagtcgctga
    33421 aacactttgt ttcccgccgt gcgatggatg ttgacggaat gggcgacaaa atcatcgatc
    33481 agctggttga aaaagaatat gtccatactc cggcagatct gttcaaactc accgcaggca
    33541 aactgaccgg actggagcgt atggggccaa aatcggcaca aaacgtggtt aacgcgctgg
    33601 aaaaagcgaa agaaaccacc tttgctcgct tcctctatgc acttggcatc cgtgaagtcg
    33661 gcgaggccac cgcagcaggt ctggcggcat atttcggcac gctggaagcg ctggaagccg
    33721 cttcgattga agagctgcaa aaggtgcctg atgttggcat tgtcgttgca tcccacgttc
    33781 acaacttctt tgccgaagaa agcaaccgca atgtcatcag cgagctgttg gcggaaggtg
    33841 ttcactggcc tgcgccgatc gttatcaacg cggaagagat tgacagcccg tttgctggta
    33901 aaaccgtggt gcttacgggc agcttaagcc agatgtcgcg tgatgacgct aaagctcgac
    33961 tggttgaact gggcgcgaaa gtcgcgggca gcgtgtcgaa gaaaaccgat ctggtgattg
    34021 ccggagaagc ggcaggttct aaactggcga aagcgcagga actgggcatt gaagtcatcg
    34081 acgaagcgga aatgctgcgt ttgctgggta gctgagatgg aaaaagagca gctgattgaa
    34141 atagccaata cgataatgcc gtttggcaaa tataaagggc gtcgcttaat cgacctgccg
    34201 gaggagtatc tgctgtggtt tgcccgcaaa gatgaattcc cggcagggaa gctcggtgag
    34261 ctaatgcaaa tcacgctgct gattaaaacc gaggggctga cgcaactggt ccagcccctg
    34321 aagcgtccgc tttaagcttt atcggcgctg ctttcctgct gtgcctgtaa ctgttcggtc
    34381 tggcgtttgt atcgacgcgc cagcaccgca cagaccatca attggatctg atggaaaatc
    34441 attaggggca gcaccatcat accgatcacc gatgtgggga acagaatgtt tgccatcggg
    34501 atgccatttg ccagactctt tttcgaacca caaaagacga tagtaatttc atctgcctta
    34561 ttgaagccca gtcggcgtgc cataaagacg ttaactacaa tcacgatagc cagaagaacg
    34621 cagctgacca ccacgataaa cagcaatgat ccccagccaa ctttatgcca gataccatta
    34681 acgacggctt cgctgaatgc tgtataaacc accaacagaa tggacgtctg gtcagttttc
    34741 gcaatccatt ttttattgcg cgacacccag tcaccaatcc acggccggga aagatgcccc
    34801 aacacaaacg gcagcagcag ttgcagcata attttaccga cctgctcaag gctgccccct
    34861 gcaccgtgaa cattcatcac cagaccaacc agcaatggtg aaaggaaaat ccccagcagg
    34921 ctggatgctg acgcagaaca aaccgccgcc gcgacgttac cgcccgccat tgacgtgaag
    34981 gcgattgcag actgcacggt agccgggaga atgcacaagt agagaaaacc ggagtagagc
    35041 atcgggtcga catttaccgg tttccaccag gcaaacagta cacccagaat cggaaacagc
    35101 acgaaggtgc tgcaaattac ccacaaatgc agtcgccagt gaccaccgcc agcaataatc
    35161 gcctcacgcg acaacttcgc gccgtgcata aagaacagca gggcaatcgc tgcggtggtc
    35221 agattttcaa agaaggggac gaaatcgcct ctggccggaa agaaagaggc cagcaacacc
    35281 accgtgatca gggttaaggt gaaaggatcg aggatacgaa aaagtttcat aaagactcca
    35341 gatgatcgat ggttctattg tgcttcttcc agtttgagaa ataaaattga tttattgcat
    35401 ccattcatga agaaaacaga tgaattattc tttaaaacaa ttaaaggttt tcgtcacagt
    35461 agcgcaggag aaaagtttta gtcgtgcagg agagcgtatc ggcctgagcc agtcggcagt
    35521 gagtcacagt gtgaaggaac tggaaaatca tactggtgtt cgcctgctgg acagaaccac
    35581 gcgtgaagtg gtgcttacag atgcagggca gcagctggct ttgcgtcttg agcgactgct
    35641 ggatgaactg aacagcacgt tgcgcgatac cgggcgtatg gggcaacaac tgagcggaaa
    35701 agttcgggtc gctgccagcc agaccatttc cgcgcatctt attccgcaat gcattgctga
    35761 aagccatcgc cgctatccag atattcagtt tgtcctgcac gatcgtccgc agcagtgggt
    35821 gatggaaagt attcgccagg gagatgtcga ctttggcatc gtcatcgatc caggcccagt
    35881 gggcgatctg caatgtgaag cgattctttc cgagcctttc tttctgcttt gccatcgcga
    35941 tagcgctttg gccgtcgaag attatgtacc ctggcaggca ttacagggag cgaaattggt
    36001 gttgcaggat tacgcgtcag gcagccgacc gctgattgac gcagcgctgg cgcgcaatgg
    36061 tattcaggcg aatattgtgc aggagattgg acatccggcg acgctgttcc cgatggtagc
    36121 cgcaggcatc ggcatcagta ttcttcccgc actggcgcta ccattgcctg aaggtagccc
    36181 actggtggta aagcgcatta cgccggttgt tgaacgccag ttgatgctgg tgcgcaggaa
    36241 gaaccgttca ctttccaccg ccgctgaagc gttatgggac gtggtacgag accagggcaa
    36301 tgcgctaatg gctggtcggg aaggggatcc gttgtatcag atatagagtg aattcatact
    36361 gccgggagcc ccggcagtaa tcaatcagaa gttaaatttg atggagtaga ttatcgcatc
    36421 ctggtaaaaa tcttcgccca gtttgtcatc cgcatatcga tactgaatcc ccgtagtaat
    36481 gtgtgatgtt gcattccacc acagtgcaac tgcgccattc agcccttctt taccgccatt
    36541 acccgctgca taggtagcgt cacggtcaaa ctcgtactcg ttccagttgg taagagtgaa
    36601 tttctcgctg cccagcataa agttgtaacc tgcaacccag ccggcgacat agccgttgtc
    36661 gccagtgtaa taggtttgat ctgtataacg cttagcaaag aacggtttga accaccaacc
    36721 gctgccggtg aaattgtagc cgataccgta caggaacata tcgtcgtgga aattcacgcg
    36781 attcgcagaa ccgtaggtgc catacgcatg caagtagaga ttaaatccgg tgtcacccag
    36841 gtaaatacgg ttggtatttt taaaggtata acgctgctcg ctacctggtt tattatggcg
    36901 accgttatag aagttttccc agtcgaagaa cccgtacatt tctccccaac taaagttagc
    36961 gccaccttca aattcaagat aaccaaaatc atctttgtgt gatttggtcg acgatttttc
    37021 ggtagtccgg ctggtccagt ccagataatg cacgccgata tctgcaaaac cgcctttaaa
    37081 ttctgcatga gatacaacag gtatcgctaa tatagaggaa agtgtcagag ttaaaagatg
    37141 ttttttcata gcataattcc ctatgccgat cgttattagt agattgctta ataaagtggt
    37201 ttgctaaaaa cgacatattg ttaatgcaaa ggaattaata tcgccaatac agtttttacc
    37261 gtggattaat agagaaaaga tataaatcca tcgatatcga atcgctattg aatgcgtgag
    37321 aaaagtgtat tggtaagagc cgggacaacc tcgctataaa aaggcgttag attccaccct
    37381 acagaaaaag gatatgtatg tctcaggttc aattttctca taacccgctg ttttgcatag
    37441 atattatcaa gacttataaa cctgatttca cgccacgagt ggcctttatt ttaggttccg
    37501 ggctcggcgc gctggccgat cagattgaga acgctgtcgc aatttcctac gaaaagctgc
    37561 cgggcttccc ggtaagtacg gtacatggtc atgcggggga gttagtgctg ggccatctct
    37621 agggcgtacc ggtggtatgt atgaaaggtc gcggacattt ctacgaaggt cgtggaatga
    37681 ccattatgac tgacgcaatt cgtaccttta agctgctggg ctgcgagcta ctgttctgca
    37741 ccaatgcggc aggctcgctg cgtccggaag tgggggcagg cagcctggtc gcattgaaag
    37801 atcatatcaa caccatgccc ggtacgccga tggtgggtct taacgatgat cgttttggag
    37861 agcgcttctt ctcactggca aatgcctacg atgcggaata ccgcgcactg ttacaaaaag
    37921 tggcgaaaga agagggtttc cctctgacgg agggcgtgtt cgtctcgtat ccggggccga
    37981 atttcgagac tgcggcggaa attcgcatga tgcaaattat tggtggggat gttgttggta
    38041 tgtctgtggt gcctgaggtt atttcagctc gccattgcga ccttaaagtc gttgcggtct
    38101 ctgcgattac caatatggcg gaaggtttaa gcgatgtgaa attgtcacat gcgcaaacgc
    38161 tggcggctgc ggagctctct aagcagaact tcatcaacct tatttgtggc tttctgcgca
    38221 aaattgcctg agaaaaacaa taagaaacgg cctcgcgggt gaggccaaat gatgcgacaa
    38281 ggaaacgatt atgagcatcg cgatgcgctt aaaggtaatg tcctttttgc aatattttat
    38341 ctgggggagc tggctggtta ccctcggctc ttacatgatt aatacccttc atttcaccgg
    38401 cgctaatgtt ggcatggttt acagttccaa agggatcgcc gcgattatta tgcctggtat
    38461 aatggggatc atcgcagaca aatggctgcg cgcagaacgt gcatacatgc tgtgtcacct
    38521 ggtgtgtgcg ggcgtacttt tttatgcggc atccgtaact gatccggata tgatgttttg
    38581 ggtgatgtta gtcaatgcga tggcgtttat gccgactatt gcgttatcga acagcgtctc
    38641 ttattcctgt cttgcccagg cagggcttga cccggtgacc gctttcccgc ccattcgcgt
    38701 ttttggtacg gtggggttca ttgtcgcgat gtgggcagta agcctgctgc atctggaatt
    38761 gagtagtctg cagctgtata tcgcgtccgg tgcgtcattg ctgctgtcgg cttatgcgct
    38821 gactttgccg aagattccgg ttgcggagaa aaaagcgacc acatcgcttg ccagcaagct
    38881 gggtctggat gccttcgtgc tgtttaaaaa tccacgcatg gccatctttt tcctctttgc
    38941 catgatgctg ggtgcggtac tgcaaattac caacgttttt ggtaatccgt tcctacatga
    39001 tttcgcccgt aacccggagt ttgctgacag ttttgtggtg aaatatccct ccattttact
    39061 gtcagtttca cagatggcag aagtgggctt tatactgact atcccattct ttttaaagcg
    39121 atttggcatt aaaaccgtca tgctgatgag tatggtggcc tggacgctgc gctttggctt
    39181 cttcgcctat ggcgatccgt caacaaccgg atttattttg ctgctgctgt cgatgattgt
    39241 ttatggctgt gcattcgatt tcttcaatat ttctggttcg gtatttgtcg aacaggaagt
    39301 tgattccagc attcgtgcca gcgcgcaggg gctctttatg accatggtaa atggtgtcgg
    39361 cgcatgggtt ggctcgattc tgagtggcat ggcagtagat tacttttcgg tggatggcgt
    39421 aaaagactgg caaactatct ggctggtgtt tgcaggatat gctctttttc tcgcagtgat
    39481 atttttcttt gggtttaaat ataatcatga ccctgaaaag ataaagcatc gagcggtggc
    39541 tcattaaaag gattcgcagc tctgctcttc agagctgctt ttatgataaa ggttaattag
    39601 tgaaagatat tttattttct tccggcgttg gttttggcat tggtgctttg tttacaatcg
    39661 tgcgcttgcc tattcctgta ccaaatgtat taccaggtat attatccatt gtgtttatgt
    39721 atgtcggata tctggtggtg aaatacttta tgccatgata atttaatacg atgtatttat
    39781 tatatggagc acttaattat ggaacgcgta tacagaacag atcttaagtt gctccgttat
    39841 tttcttgccg tagcggaaga gttgcatttt ggccgcgcag cagcgcgttt aaatatgtct
    39901 cagcctccgc tcagtattca tattaaagag ctggaaaatc aactcggcac gcagcttttt
    39961 attcgccatt cgcgcagcgt cgtactgaca cacgcgggca aaatcttgat ggaagaatcg
    40021 cgtcgattgc tggtgaatgc taataatgta ttggctcggg ttgaacaaat aggtcgggga
    40081 gaagcagggc ggattgaact cggcgtagtg ggaacggcta tgtggggacg gatgcgcccg
    40141 gttatgcggc gattcctcag ggaaaatcct aacgttgaag ttctttttcg cgaaaagatg
    40201 cccgcgatgc aaatggccct gctggaacga cgcgaactcg atgccgggat ctggcgaatg
    40261 gcgacagaac caccgacagg ttttaccagc ttacggttgc atgaatcggc gtttctggtg
    40321 gcgatgcctg aagagcatca tctctcatca ttttccaccg ttccgctgga agcgctacgt
    40381 gacgagtatt ttgttacaat gccgcccgtg tacactgact gggatttttt gcagcgagtt
    40441 tgccagcagg tgggattttc accggttgtt attcgcgaag ttaatgaacc gcaaacggta
    40501 ctcgccatgg tcagtatggg tattggtatc acattgatag cggacagcta cgcacaaatg
    40561 aactggccag gtgtcatttt ccgtccgctc aaacagcgca tccctgcaga tttatatatt
    40621 gtttatgaaa cacagcaggt gacgcccgcg atggttaagc tgttggctgc attgacacag
    40681 taggtgagga gacaggaatt gccggaaata gtcgtctgct aagtgaagcg catcaggcaa
    40741 tttcgcaaat cgca
//
LOCUS       DAZFDP010000025        40445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFDP010000025 DAZFDP010000000
VERSION     DAZFDP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..592)
                     /locus_tag="ACHJ1G_001458"
     CDS             complement(<1..592)
                     /locus_tag="ACHJ1G_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO4763341.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSF"
     gene            complement(851..2104)
                     /gene="amiC"
                     /locus_tag="ACHJ1G_001459"
     CDS             complement(851..2104)
                     /gene="amiC"
                     /locus_tag="ACHJ1G_001459"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4763342.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2336..3667
                     /gene="argA"
                     /locus_tag="ACHJ1G_001460"
     CDS             2336..3667
                     /gene="argA"
                     /locus_tag="ACHJ1G_001460"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4763343.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3729..5555)
                     /gene="recD"
                     /locus_tag="ACHJ1G_001461"
     CDS             complement(3729..5555)
                     /gene="recD"
                     /locus_tag="ACHJ1G_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4763344.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5555..9097)
                     /gene="recB"
                     /locus_tag="ACHJ1G_001462"
     CDS             complement(5555..9097)
                     /gene="recB"
                     /locus_tag="ACHJ1G_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4763345.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9090..11978)
                     /gene="ptrA"
                     /locus_tag="ACHJ1G_001463"
     CDS             complement(9090..11978)
                     /gene="ptrA"
                     /locus_tag="ACHJ1G_001463"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4763346.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHLAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12154..15522)
                     /gene="recC"
                     /locus_tag="ACHJ1G_001464"
     CDS             complement(12154..15522)
                     /gene="recC"
                     /locus_tag="ACHJ1G_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4763347.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15535..15858)
                     /gene="ppdC"
                     /locus_tag="ACHJ1G_001465"
     CDS             complement(15535..15858)
                     /gene="ppdC"
                     /locus_tag="ACHJ1G_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4763348.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15843..16250)
                     /gene="ygdB"
                     /locus_tag="ACHJ1G_001466"
     CDS             complement(15843..16250)
                     /gene="ygdB"
                     /locus_tag="ACHJ1G_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4763349.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16247..16810)
                     /gene="ppdB"
                     /locus_tag="ACHJ1G_001467"
     CDS             complement(16247..16810)
                     /gene="ppdB"
                     /locus_tag="ACHJ1G_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4763350.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16801..17271)
                     /gene="ppdA"
                     /locus_tag="ACHJ1G_001468"
     CDS             complement(16801..17271)
                     /gene="ppdA"
                     /locus_tag="ACHJ1G_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4763351.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17455..18249)
                     /gene="thyA"
                     /locus_tag="ACHJ1G_001469"
     CDS             complement(17455..18249)
                     /gene="thyA"
                     /locus_tag="ACHJ1G_001469"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4763352.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18256..19131)
                     /gene="lgt"
                     /locus_tag="ACHJ1G_001470"
     CDS             complement(18256..19131)
                     /gene="lgt"
                     /locus_tag="ACHJ1G_001470"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4763353.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19282..21528)
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_001471"
     CDS             complement(19282..21528)
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_001471"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4763354.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21541..22071)
                     /gene="rppH"
                     /locus_tag="ACHJ1G_001472"
     CDS             complement(21541..22071)
                     /gene="rppH"
                     /locus_tag="ACHJ1G_001472"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4763355.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22424..22570)
                     /gene="ygdT"
                     /locus_tag="ACHJ1G_001473"
     CDS             complement(22424..22570)
                     /gene="ygdT"
                     /locus_tag="ACHJ1G_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4763356.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22756..23445
                     /gene="mutH"
                     /locus_tag="ACHJ1G_001474"
     CDS             22756..23445
                     /gene="mutH"
                     /locus_tag="ACHJ1G_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4763357.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23514..24227
                     /gene="ygdQ"
                     /locus_tag="ACHJ1G_001475"
     CDS             23514..24227
                     /gene="ygdQ"
                     /locus_tag="ACHJ1G_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4763358.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24365..24583
                     /gene="ygdR"
                     /locus_tag="ACHJ1G_001476"
     CDS             24365..24583
                     /gene="ygdR"
                     /locus_tag="ACHJ1G_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4763359.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24691..25731
                     /gene="tas"
                     /locus_tag="ACHJ1G_001477"
     CDS             24691..25731
                     /gene="tas"
                     /locus_tag="ACHJ1G_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4763360.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25763..26956)
                     /gene="lplT"
                     /locus_tag="ACHJ1G_001478"
     CDS             complement(25763..26956)
                     /gene="lplT"
                     /locus_tag="ACHJ1G_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4763361.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26949..29108)
                     /gene="aas"
                     /locus_tag="ACHJ1G_001479"
     CDS             complement(26949..29108)
                     /gene="aas"
                     /locus_tag="ACHJ1G_001479"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4763362.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29694..30725
                     /gene="galR"
                     /locus_tag="ACHJ1G_001480"
     CDS             29694..30725
                     /gene="galR"
                     /locus_tag="ACHJ1G_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4763363.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30732..31994)
                     /gene="lysA"
                     /locus_tag="ACHJ1G_001481"
     CDS             complement(30732..31994)
                     /gene="lysA"
                     /locus_tag="ACHJ1G_001481"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4763364.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32116..33051
                     /gene="lysR"
                     /locus_tag="ACHJ1G_001482"
     CDS             32116..33051
                     /gene="lysR"
                     /locus_tag="ACHJ1G_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4763365.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33038..33730)
                     /gene="ygeA"
                     /locus_tag="ACHJ1G_001483"
     CDS             complement(33038..33730)
                     /gene="ygeA"
                     /locus_tag="ACHJ1G_001483"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4763366.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33859..35277)
                     /gene="araE"
                     /locus_tag="ACHJ1G_001484"
     CDS             complement(33859..35277)
                     /gene="araE"
                     /locus_tag="ACHJ1G_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4763367.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35592..36353)
                     /gene="kduD"
                     /locus_tag="ACHJ1G_001485"
     CDS             complement(35592..36353)
                     /gene="kduD"
                     /locus_tag="ACHJ1G_001485"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4763368.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36383..37219)
                     /gene="kduI"
                     /locus_tag="ACHJ1G_001486"
     CDS             complement(36383..37219)
                     /gene="kduI"
                     /locus_tag="ACHJ1G_001486"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4763369.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37506..38687)
                     /gene="yqeF"
                     /locus_tag="ACHJ1G_001487"
     CDS             complement(37506..38687)
                     /gene="yqeF"
                     /locus_tag="ACHJ1G_001487"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4763370.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38942..40171
                     /gene="yqeG"
                     /locus_tag="ACHJ1G_001488"
     CDS             38942..40171
                     /gene="yqeG"
                     /locus_tag="ACHJ1G_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4763371.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        10064 a        10947 c         9767 g         9667 t
ORIGIN      
        1 taaatgaact tctgtccacc cttctgttat aaacaacttc aataagctcc gtgatttttt
       61 gagacagctc aataatcctt actgattcat aatcaactga attttcataa ttatttaaaa
      121 tatgcaatgc tataaaacca atctcatcat ccggtagttg aacgctaaat tgctcattaa
      181 tgattgttaa tgcatcacga cttattgcat actctgcttt gtacaacgct ttgatttcct
      241 caaggaaaac attattaatt tgtatgcctg agacgactcg ggatattgca ttcgaaatgt
      301 gatcagcaag aatcagtagt agcgatggat tgagtttttt ttcgagatta ctctccgcat
      361 aatgcactat cttttcggca acaaaaatat actcaatacc cacatgctca atcaatttgt
      421 agagtttatt tttttgctca tttctgacat aaaaaatccg gtttgcagga tgctcaggga
      481 ccgacatacc atatttttta ttgtaaccaa cgccaggccc agaaatgata acctcctgtc
      541 cgttcaccga tgcctgtaca caattattgt tcatgacttt ttcgatgatc attccatggc
      601 tcccaaaaaa aaagcaaggc atgtcagaag taataattac ttcaaacatt gccttgcctg
      661 atttaacagt tacaagataa ttaactaatg cgtatacctt acacggaatt gtgcttaata
      721 aaagagatga caggcttatt ttttaagccg atattcaaat attcgacagc agtcacaaaa
      781 ccatcgttca acatttatgt aaataatatt tattcaatga aacataaatt aattgaataa
      841 attaaaaaca tcatcccctt ctcgccagcg tcgccccatc ggcaaaatac gctttaatcc
      901 ccgcaagaat agactccgca acttcctgct gaaaggtcgc cgttttcagt ttacgctctt
      961 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
     1021 gtacggcaaa cccggcctgt tcaacctgat ttttatgcag cttgttgatt ttaccgagct
     1081 tattcagtac cgctttacca aacttaaggc tgtcagcaat ggtcagcgac tgcaccatat
     1141 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
     1201 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga
     1261 gcgcaaacac agaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
     1321 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
     1381 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
     1441 aacggcgagc tatttgcaat accacatctt tttctcgcgt tttgtatttc cccaccgcac
     1501 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
     1561 ctttacccgg ttgtggacca ctttgtgctg gcggcacctg cttttcgagg tcgcctttgt
     1621 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
     1681 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
     1741 ttacgttttg ctttaattca aaaaccatcc gtacggtctg cgggtcaaat tgcccgacgc
     1801 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     1861 agttcaggtt tacatcttcg atatccacca ccacgcgttc cgggttactc aacgcgaact
     1921 gcttatattt cagctgacga tttgattcta ccgtcacacg ggtgtagctg gacgcaggcc
     1981 agacgcgcac cgccacgacc tggctgaccg cagccagact gacctgactt acgctcaata
     2041 gccacatggc acccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
     2101 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     2161 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     2221 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
     2281 gaaaaaacag aataaaaata cactaatttc gaataatcat gcaaaggggt gtgccgtggt
     2341 aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac
     2401 ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
     2461 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
     2521 ctatggcgca cgtccgcaga tcgacgcaaa tctggccgcg catcaccacg aaccgctgta
     2581 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
     2641 aacattgcaa ctggacatca ctgctcgcct gtcgatgagt ctcaataaca cgccgctaca
     2701 gggcgcgcat atcaacgtcg tcagtggcaa ttttattatt gcccagccgc tgggcgtcga
     2761 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatcca
     2821 tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
     2881 cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc
     2941 tgaaaagatg attggttttt gctcttccca aggcgtcact aatgacgacg gtgatattgt
     3001 ctccgaactt ttccctaacg aagcgcaagc acgggtagaa gcccaggaag agaaaggcga
     3061 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
     3121 gcgtcgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc
     3181 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
     3241 aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
     3301 ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg
     3361 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
     3421 aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct
     3481 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattatttg tgctgaccac
     3541 gcgcagtatt cactggttcc aggaacgtgg atttacccca gtggatattg atttactgcc
     3601 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
     3661 ggggtaagcg atgaaaatcg ccggatgcga catgcgtaac actcgtgcgt cgcatcaggc
     3721 aattacgttt attcccgcga actaaacaat gccgccagac cactgcgccg ctcagtgcgc
     3781 gtggcgattg ccgcacttaa tatgcgctca tcggcataca gcgacagacg gcgacgcgcg
     3841 cgggtcactg cggtgtaaac cagctctcgc gttactaccg gcgtgcgttg gctcggtaaa
     3901 atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgtc
     3961 gtttcgtgct ctggcaggcg gctcggttgc acagacttaa tattgccgtc cggcatcgca
     4021 aaccaaacgc gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac
     4081 ccaagcgcgc tgtcattacg ggcaatcatc accggtcgac cttcgtacca acgagagtgc
     4141 ggatgacgat gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca
     4201 gccacgccaa acggcccttc ccgcagggcg cacaaaagtt ggtactcatt gaacgcctga
     4261 atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc ataacccgca
     4321 agagcttcct caagcatcgc aatataatct tctccgctct gtaaaagtcg tttttcgata
     4381 tcagtaaaat cctgctgaaa aacggttttc actgccgttt tatcaccacg gttgatcgcc
     4441 gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag
     4501 agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agtccccgtc
     4561 aggcggctta gctgcctggc acgctcggcg gtaaagcccg cgttggcata agcgcagata
     4621 tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc
     4681 actcgcgcat gatctggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt
     4741 gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa
     4801 cgctggctac ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg
     4861 cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt
     4921 aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg
     4981 gccatttgaa ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg
     5041 ccgccggaaa ccaccgagat ccgccgcgtc agcgccactg ccgctgcaac tttttgccag
     5101 ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca
     5161 acctcaatgg catgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac
     5221 atgcgattca aataaagacg atcgccacag aggaccatcg gcgtgggttc atctcccctg
     5281 ctgaccgctt gagaagccag caagcattct tcccaatttt gtagctcacc gatttcactg
     5341 acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga aagcggcaaa
     5401 cagacgtgtc cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga
     5461 tgttcatctc ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc
     5521 tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccagg gtcataccgg
     5581 caaacatctc atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt
     5641 gttgcggatg ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgac
     5701 gatcatagtc gtaatcagca atgcgatggc gcagataacg atgcagcgcc agggtataaa
     5761 gctgatattg cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt
     5821 aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt
     5881 cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca
     5941 gcggcgggca gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg
     6001 cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg
     6061 aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc
     6121 actcggtcaa caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta
     6181 cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg
     6241 tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc
     6301 tgaccacgcc tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac
     6361 cacgctgctg caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta
     6421 gcgtcctcgc attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac
     6481 cagtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa
     6541 gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga
     6601 cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc
     6661 aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt
     6721 cgacctccgc aagctcgacg ctttctggcg cagcgttaag atccagaaca gcctcaaacg
     6781 agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc
     6841 agaccagcgg atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt
     6901 tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt
     6961 gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgtagca
     7021 gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt
     7081 tttctgcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc
     7141 gccagatttg ccgataacca tcgaactctt caactaccgc atcccacgca tgttcgtcat
     7201 tattcagcgt ttcgatatcc agcgcgttca gccccatcat tgacgttgcc agcgcactgc
     7261 gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa caaccaaagc atttcctgcg
     7321 cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg atttccagca
     7381 acgttaaggc atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acactgatgt
     7441 ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc
     7501 cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgataat
     7561 cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac
     7621 ctttaaacac aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta
     7681 tttcgcgaaa catgaacgcg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca
     7741 ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg ggcgtgaact tcactacgcg
     7801 ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc
     7861 caatcagcaa caatgcggtt tccggctgat ggtgccagat acggcgaaaa attcggtact
     7921 gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg
     7981 ccgctgccaa cacctcaccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt
     8041 catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag
     8101 ccaatgcgcg ggtgatcacc agatcgcgga tcgacaatgg ttctgcaagc agttgatcga
     8161 tcgcctcaaa cagtggatgt cgcggggttt cccccccggc cttcgtgcga tcttctaaga
     8221 aacgctggga gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg
     8281 cccaggcgct gatcttttcg atccatttag cctgattgct acggttaaac ttgcgtcgat
     8341 caataccaga agattcgatc agcgcatcaa gttcacccac tgcgtcgcgc cactgctgtt
     8401 ttaccgtatc aatacgcgcc acaatttgcg cgtggcggga agccagcgtt tcatcatcgg
     8461 gcggcggtgc tttgataacc ggtgcttcgc cttgcagata acgattaata tcgcgcagca
     8521 acgcctgcgg ccctttccag gtttcaaaga ccaccagggc aatttcacgc ggcagcgggt
     8581 agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa
     8641 tcagctgctg ctcaaacagc ataccggatt caaaggcatt caggttgagc atgcgctggc
     8701 aaaaaccgtg aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc
     8761 actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gcgttcgtac agtggattgt
     8821 cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccac
     8881 gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acggtcagcg
     8941 ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag gcgcaaatag agcgccgcaa
     9001 tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca
     9061 agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt
     9121 gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg
     9181 gctttcccgt tctggctgcc ggaaatctgc gacagaatag ccatgccttg cggctcgacc
     9241 accgccagat ggaagaaatc agcaagtttt tgcggcgtca gcagttttat ctgggccacg
     9301 attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat
     9361 gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt
     9421 tgcgcaaact cttctggctt catcgcccgc aattttgcct ctgcggttgg gaaaaacgcc
     9481 ttgtaacgct cccacaagaa ggaaggctgt ttatcattgc tttgcaaaag gaagcccatg
     9541 ccccactgac gccccacgct cattggaaat gcaaacacgg catagcccaa ttgttcttcg
     9601 gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct
     9661 gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg
     9721 ctgttaccag ctttttcaaa gatgacggat tgttttttat cgaccacgac atctttgtta
     9781 cgacaccact ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt
     9841 gcctggtcct cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag
     9901 gcgtcgcgat aggccagcac ctctttcaac gtaatggagg gcaaaatttt gcgccgttca
     9961 tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcgaac
    10021 gctttgccct tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc
    10081 tgatcttccg tggcggtata gctaaagtag ccttccagca atgcctggaa cagctgcggc
    10141 agatgctggg tgtaaccatt agcattaacc ataaggccgt tgttagcgtt ggtggaaaaa
    10201 cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa
    10261 tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa
    10321 atcaggctga catcggcttt tggctcgctg gcaaaataac ggcttggcgc atacaccacg
    10381 cgcagattcg actcatcaac aatcagctct ggatggtcat atttcttgtc tgacttaatc
    10441 agcgagaaat cgtcaggaat ataagggtta agctctggca aagagagcgc aatgtcggcg
    10501 gcttttttct gccagtcggc gaaagtttgt gcgctgattt tatcgacctg atacggcgca
    10561 tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc tgatatacca gatacgcgca
    10621 ttctgcggcg tcatcatcgc cagacgttcc tttactgctt tagcatcgta ccgatcggca
    10681 atattgactg catccagcgt atgctcaaca ggaacgcgaa tcatggtatc tgccagccat
    10741 tcgacgtaat ccatatcacg ggtgattgac ggataacgga agtcgatatc cagcacattc
    10801 gccagttcat cgaagtattg tttatcgatc cctttttcac gtaacaaatt gaggtagcta
    10861 aaaatagccg ccacaacctg atcgcgattc gccagacctt tatcggttaa agacgcagag
    10921 atcgctaata cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca
    10981 actaatccct gcttttgcag ccagtcggaa agtgtacctg ggctgcgatt accaatcaga
    11041 taggtaatca attcatcggt tttgctacgg aatttcgccg agttgttatc aatacgaaac
    11101 tctacgcgca acactttacg cggcagcgcc gggacgtaat gaatgataat gcccttttgc
    11161 gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga
    11221 ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg gtttattgct gtaaataacc
    11281 gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc
    11341 ggattaccag gtttgtcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg
    11401 tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc
    11461 gccatggtta attcagcgtt cactgcatta cgctcacgtt cggcgtattt cttgtcgagc
    11521 aagggttctg caatagcatc agccaggcgg tctaccgcac caggcaaggc gtcgttctca
    11581 acttccagat agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc
    11641 attttgagat attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc
    11701 atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg
    11761 ggcaccacca gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc
    11821 ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg
    11881 gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaact
    11941 aacaacaata atgctttgaa ccaggtgctg cggggcattc acggacctca taatcaactt
    12001 aattttctgt ccagattcaa cacgttaacg caaatcatct gccagaattt aatcttgtgc
    12061 tgcacgagtc agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt
    12121 aagcggatag attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg
    12181 taacaggaaa cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg
    12241 ccagagcctt tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta
    12301 agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc
    12361 gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa
    12421 tggcgcggac atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg
    12481 ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc
    12541 accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc
    12601 cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca
    12661 gccagttatc tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg
    12721 acaggcaatg actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat
    12781 ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc
    12841 ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag
    12901 cccttccaga ataaatggct cggtgtctgg gatttcgctg tcttcagtac ggaagttcac
    12961 ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc
    13021 cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt
    13081 accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg
    13141 gtagttttgc ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg cttttaccct
    13201 tgcctcgctt tcatcacagt tgagcgcttc atcgtccggt agataatgac tttgcccgat
    13261 gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga
    13321 acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag
    13381 gtagcggtcg tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc
    13441 aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac
    13501 cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag
    13561 aaaacgctgg ctgatacgtt cttgatccag acgctgcgcc agttcatcac gcaatagtga
    13621 cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg
    13681 ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag
    13741 catatcgcga caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg
    13801 ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc
    13861 gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta
    13921 gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc
    13981 cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg
    14041 gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc
    14101 cagtaatgcc agcacgtctt ccgacacaaa gcggctgtct ggcaacgata acaggctgat
    14161 aaacgcttcc agtaccggat gcgactgccg cgcccgacgg tcggaaatgg cgtaaggcag
    14221 gtaacgatcc gcaggtgcgc taccaaacac agcctgaata aacggactgt agctgtcgat
    14281 atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc gggtcttctt ccagcatcgc
    14341 cagcaggcga tcgtgcaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt
    14401 gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat
    14461 gttcacacca gcaacggcac ggttttccag ttccagaatg tcagactgaa tgttatgcag
    14521 caggttatct ggcgtgacat cgacaaaggc gtccagctcc tggctgctct ccaggtcaga
    14581 aaggagataa atgtagtcgc gcccaagctt accccatgaa gccagcagcg ggttgccgac
    14641 atcctgttca ccatcgctgt taaagagctg cccggcattt tcgctatcac gaaatagcgg
    14701 caattcgcga tcttcaacac tgtgtcgacg ctgacgggtc agcagtttcg ccagataagc
    14761 aggatcttta atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc
    14821 aatatgttta cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc
    14881 gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc
    14941 gataaagcgc tgatagagat tggcgcggtg ccaacgcggt tgtccgagtt catgggtata
    15001 ttccaccagc gccttccaca acggcgcttg ccaggcctgt gcttctccca gcccttcaac
    15061 caaatgtcct gtttcccact gtgccagcca ctccgggcga tagaccagat actggtcaaa
    15121 caggtccgcc gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagccag
    15181 ataatgccgc aacagggtaa agtcttcgcg atccagcaat tgcggcagca gagtcatcag
    15241 tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatctccg gtaacacccg
    15301 gacgaacata tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatacc
    15361 aaacttttgc gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat
    15421 catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc
    15481 ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg
    15541 acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt
    15601 aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc
    15661 aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta
    15721 ctggtttctg ctggcgagac tgttcattaa tgttcgctgg atacccgata acgcagtgac
    15781 aatcatcacc atcagcacca tcgcaaccat tacctccggc aggctaaagc cttgttgatt
    15841 cttcagggaa gctgacataa cgccctctct ttcagcggac aaaaatcgct ccagccgcgt
    15901 ggcgaaaaaa caatgtttcc atcaatgact tcgcctgttc gccacaacga aacgccttca
    15961 taaccggcaa ccaataaggc ttcattatct gccagtaaac gcaaacaaac ccgggcatcg
    16021 gttccagcgt actgcgagca ctgaactgcg ggctgcgtct gccaggagtg cattttcccc
    16081 cacgccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc catgctcacg
    16141 cgagaggcaa aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc
    16201 agcagcatca ggaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc
    16261 ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca
    16321 tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt
    16381 cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg
    16441 caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcagactctt
    16501 ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt
    16561 caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc
    16621 tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct
    16681 tacgggtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgca gcccccagca
    16741 acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt
    16801 ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac
    16861 caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt
    16921 gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg
    16981 tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac
    17041 aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt
    17101 ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccatagccg
    17161 ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca tgaccaaaat
    17221 cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt
    17281 atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga
    17341 ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaacg
    17401 gagggtaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc gtaattagat
    17461 agccaccggc gctttaatgc ccggatgtgg atcgtagcct tcaatctcaa agtcttcgaa
    17521 acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca gcggacgcgg
    17581 ttcgcggctt aattgcagat gagtttgatc catatgattg ctgtacaggt gcgtgtcgcc
    17641 ccccgtccag acaaaatcgc ccacttccag atcacactgc tgcgccatca tatgcaccag
    17701 taacgcgtag ctggcaatgt taaacggcag gccgaggaaa acgtcacagg agcgctgata
    17761 aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaacgcat ggcacggtgc
    17821 cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc ggcgcgaatc
    17881 cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg tcaatatgac gaccatccgg
    17941 cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccgt tttcatcggc
    18001 ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag tgtcgccctg
    18061 cagaaaccac agcagttcat ggatgatgga acgcaggtgg caacgtttag ttgtcaccag
    18121 cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa gcgttccggt
    18181 tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca gttctaaata
    18241 ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg cccagaccat
    18301 catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgtcccatgc tgatgtactg
    18361 cacccaggca ccagtaaact gcgcgtcggg ctggcggaaa aactcaacaa tgatgcgaaa
    18421 cgcgccgtaa ccaatcagga acaaacctga gacagctccc attgggcgcg gtttacgaat
    18481 atacaggttg aggataataa acagcactac accttccagc aacagctcgt aaagctgtga
    18541 tgggtggcgc ggcagcacac cgtaagtatc gaaaatggat tgccactgcg ggttggtttg
    18601 cagcagcaaa atatcttctg tacgggagcc agggaacagc atggcaaacg ggaagttcgg
    18661 gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg caccaagacc
    18721 aaacggaatg agtggtgcga taaaatcaga gacctggaag aaggaacgtt tagtacggcg
    18781 ggcgaagata atcatcacca cgataacgcc aatcaggccg ccgtggaagg acatgccgcc
    18841 gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaagt tgtagaacag
    18901 aacataacca atacgtccac cgaggaagac gccgaggaag cccgcataga gtaagttttc
    18961 aacttcattt ttggtccagc cactgcccgg acgattcgcc cgtcgtgttg ccagccacat
    19021 tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca cgggtcctat
    19081 tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca tctgtcacca
    19141 caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg caggtttcag
    19201 gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg ataccgttaa
    19261 aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca tgccacgacg
    19321 ctccataaag gctgcaacct gatggcgaac ttcggtcgcc agttgcgctt ccagactacg
    19381 ctgcgcaaga ttttctgctt cggcataatc aatgcgccgc agcaggtatt ttacccgcgc
    19441 tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga ttgccacgca
    19501 catgggatcg cccgccattt caccgcacaa acggagatcg attccatgta tttccgcttc
    19561 ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcat aaatgttcgc
    19621 cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt tggtgccaac
    19681 agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg acggcacttc
    19741 cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct cgacttcacg
    19801 tccggcacgt tcaatcaggc ggcgtgcttc atcaacttca tcgaggcttg tgaccatcgg
    19861 caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc gcacctggat
    19921 caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca ggcatggatt
    19981 ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca gcgtacgcaa
    20041 ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca cctgttcttc
    20101 ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa gaccgatgcc
    20161 atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccagcat tgagcatgac
    20221 ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt ccgccagacg
    20281 gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta ccggctccgg
    20341 atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat gcagcaccga
    20401 aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca tgatcgcagc
    20461 atgggagttg gctgcgccat ctcgcacgac aacaccgact aagcgatcct ggggcagctc
    20521 agcaagagtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg gccaggcgtt
    20581 cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg cacgtaaatc
    20641 gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg caaatttttc
    20701 aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg caaacaattc
    20761 gcgacgcagc cgggtgtccg aaagtaggtg cgagtaaaga tcgaaaatag ccgccgtttc
    20821 tttttgtgct ccggcggcaa agcgtttgct gtagcggcga aactcgtttg ccgcttcttc
    20881 cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg cctgatacac
    20941 ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca caccaggtgc
    21001 tgccggtaat gcgcggattc gcgtctggcg atattgccca aacagggcag tcaactgcga
    21061 ctgggaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt cttcactttc
    21121 gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact ggcgacgttg
    21181 aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg ggatgtattt
    21241 gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc tgccaaccag
    21301 gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt ttttcagccc
    21361 ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga ccgaacagac
    21421 ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct cattcaggcg
    21481 tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa ttggcgtgac
    21541 ttaacctctt ttacgtcgat aagcagatgc gttttgtggt tttggcgtat tttcctgcag
    21601 tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac gtttaaatga
    21661 caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa actctggtgt
    21721 actgctggtt tgcatattga tttctgcatc gccgcttacc agctgcaaga gaaaccattt
    21781 ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt tcggtaattt
    21841 gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc ttaatcctac
    21901 ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt tgattccgcc
    21961 ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct gcccctggcg
    22021 attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca ccggactacc
    22081 tcaaaataaa gctttatata cgaatgattg tttcatactc caggaagacg gtaaaccact
    22141 ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt gcagagttat
    22201 aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata gagttgtgaa
    22261 gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga tatagacatt
    22321 ttaaaatatt cattattagt caatgcatta catgtaattt gagtaatgaa aaagagtaat
    22381 tcgtgaccca gctcacacct ctcatttacg ggttggcgaa agatcaacca atgccgtatt
    22441 tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga gtttcgtcgc
    22501 acgtcaaggc tgtaaatgga aacagtagtg gaggtttttc acagttatcc cagctttctg
    22561 tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa aacctattgc
    22621 agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcaga gaattgaaca
    22681 acacatatgg aaaaagttac actgcgaata ttcggcacat aattgctgtt tgttttttaa
    22741 ccaaggtatc atgacatgtc ccaacctcgc ccactgctct ctcctcccga aactgaagaa
    22801 cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact ggcggcactt
    22861 gccgggctgg ttacgccaga gaatttaaaa cgcgataagg gctggattgg cgtgttactg
    22921 gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc tgctctgggc
    22981 gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac aacattcgtt
    23041 tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca tgtgcgccac
    23101 aagctcaaac gcgtactgtg gataccggtt gaaggcgagc gcagcatccc gctggcgcag
    23161 cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg acagctacgc
    23221 gaagactggg aagaattaat ggatatgatt gttctcggtc aggttgagcg gatcaccgct
    23281 cgtcacgggg aatatttaca gataagaccg aaagcagcga atgcgaaagc gcttaccgaa
    23341 gccattggtg cccggggcga acggattctg acgctgccac gcggctttta tttgaagaag
    23401 aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc gctttgacct
    23461 gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt tagatgttat
    23521 ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacgctgacg ctgctggaga
    23581 tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag cttcccacag
    23641 cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg cgtctggcgc
    23701 tgctggcatc aatcgcctgg gttacgcgcc tgacaaatcc gctttttaca atattcagtc
    23761 aggaaatttc tgcccgtgat ttgattctgc ttctgggtgg cttgttcctt atctggaaag
    23821 ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa acacgcgttt
    23881 catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttt agcctcgact
    23941 cggtgattac ggctgtgggt ctgtcagatc acctgtttat tatgatggca gccgtggtga
    24001 ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc gaacgccatc
    24061 cttcggtaaa aatgctggcg ctctctttcc tgattctggt gggctttacc ctgattctgg
    24121 aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc ttctctattg
    24181 cggttgaaag cctcaacttg attcgcaaca aaaagaatcc gctctgatac tccgtacgct
    24241 ctcctgcggg agggcgtact cactcctttt accctcacag tttaagattt tcctgctttc
    24301 aagatatata acgtcggttt ataaacagac tattatcata ggtaggccaa atgaggataa
    24361 ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg
    24421 gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa
    24481 acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat
    24541 gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc
    24601 cggctggcct taagattttt ctctttccct tttcccttcc ctctgccatt tttatattcc
    24661 ttatgtcgtg attataaaaa ggaaacggct atgcaatatc accgtatacc ccacagttcg
    24721 ctggaagtca gcacgctggg gcttggcacg atgacgtttg gtgaacagaa cagcgaagcc
    24781 gacgcccacg cacaactcga ctatgccgtc gctcagggca ttaaccttat cgacgttgcc
    24841 gaaatgtacc cagtacctcc gcgccccgaa acgcaagggt taaccgaaac ctacgtcggc
    24901 aactggctgg cgaaacatgg cagccgcgaa aagttaatta tcgcctccaa agtgagcgga
    24961 ccgtcgcgca ataatgacaa gggcatccgc ccggatcagg cgctggatcg gaagaatatc
    25021 cgcgaagcgc tgcatgacag cctcaagcgc ctacagactg attacctcga tctttatcag
    25081 gtgcactggc cgcagcgccc gaccaactgc ttcggcaaac tcggttatag ctggacggat
    25141 tctgcgcctg cggtttcgct gctggatacg ctggacgcac tggcagagta ccaacgcgcg
    25201 ggaaaaattc gttatatcgg cgtgtcgaac gaaactgcat ttggcgtaat gcgctacctg
    25261 catctggcgg acaaacacga tctgccgcgt attgtcacca ttcagaaccc ttacagtctg
    25321 ttaaaccgca gttttgaagt aggtctggca gaagtcagcc agtatgaagg ggtcgaactg
    25381 ctggcctatt cgtgcctggg tttcggcacg ctgaccggga aatatctcaa tggtgcaaaa
    25441 cccgctggcg cacgtaatac gctctttagt cggttcaccc gctatagcgg tgagcaaacg
    25501 caaaaagccg tcgcggcgta tgttgatatc gccagacgtc atggcctggc ccctgctcag
    25561 atggcgctcg cgtttgtacg ccgtcaaccg tttgttgcca gcactctgct gggcgcaacc
    25621 acgatggatc agctgaaaac taacatcgaa agtttgcatc tggagttaag cgaagacgta
    25681 ttagctgaaa ttgaagcggt gcatcaggtt tatacttatc cggcaccata agattaacgc
    25741 cggttaaaac cggcgttaaa tattaatgac ggcgctgcca gatccacagc gccgttattg
    25801 ccagcgcaaa cagcgcaccg aagccaatgc caatgggcac gaccgggatg cctatcatta
    25861 ccgccagcga gtaaatgccc agcatcaaca acatggcgct gttttcgcca aggttttgta
    25921 ctgcaatcgc attccccgcc ccgacgcttt ttttaccccg ctcctgtagc aacgcattga
    25981 gcggaacgac aaaaaagccc cccatcacgc caatcagcat caacaaggca taggctggca
    26041 gcagctcgtg ttgcagggaa aaaatcagta ccaccacgcc aatcaaaatc ccggctggca
    26101 tacagcgtga cacggtttcc agcgtaacta acttcgctgc cgcacctgcg ccaaccacga
    26161 taccaatcgc taccatcgcg ttgagatagg tgggcgtagc gttatcggta atgcccagcg
    26221 ccaccggtac ccacagcacc aacaggaaac gcagcgtgac acccgctccc cagaataaac
    26281 tggtgcccac cagcgaaaaa cgcgtttcac cattgcgcca tagcgaggtg caggcattca
    26341 ggaaactgcg ggtcatgttg atgagattcc aggactgccc cggacgcgcc gccgccagtt
    26401 tgggaatgta gatattggca acgaccgcac caccgtaggc cagtgcgcat gcggccaggg
    26461 cgacgaggac atgccagtca gccagcacac caccggctac ggaaccgagc aaaatcgccg
    26521 ctatggtaga agcttccatt aaaccgttag ctttcactaa cttactaccc gtggttaatt
    26581 cgccgagaat accgtatttc gccggtgaat aggctgcagc accaacaccc accagcgtat
    26641 agccgagaaa cggattgata ccaaagcaga tactggctgc gcccagcagc ttcaggccgt
    26701 tggcaaacat catcacccgg cctttggcga agctatccgc cacctgcccg acaaacggcg
    26761 caaaaagaat gtaagcacct acaaacacca tttgcaggat gggctggctc cactccggat
    26821 agaactgcgc tttcagtaac gccagagtgg caaacagtag ggcattatcg ccaaacgcag
    26881 agagaaactg cgccacgata accgctttca tccccttcga ccacaacgaa gtgttagtgt
    26941 gcactgactc actcatcgtg ttgttccgct tcgtctaccc agcttttcaa cgtgacaaag
    27001 tcaggtttgc cgctgccaag taatggcatc tgtttcagat agcgaatatc gcgcggtaca
    27061 gcaagctccg gcacgccgtg ctcgcgggca tactgttgca acttatcgcg cgtcagttcg
    27121 ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg catcgctctt aatcgcagtg
    27181 gcatggactt tatctggcga aacaccaagt gccagttgtt ccaccatttc cagcgacacc
    27241 atttcgcctg caattttggc aaagcgtttt gcgcggccct gaatctgcac aaagccctgc
    27301 tcgtcaaaac gcacaatatc gccagtgtca taccagccgc gctccatttc gccgcgaaca
    27361 ttctcggcgg tgggcacttc cagtacacct ggcttctcca cccgcagata gccgttcatt
    27421 atgttcggcc ctttcagttg caggcgtccg ccctcttcga taccaggaac cgacaacagg
    27481 cgcgcatcca ttcctggtag aatacgccct accgtaccgg gtttcgccgc catcggtacg
    27541 ttgatagaaa cgacaggcgc gcactcggtt acaccatagc cttcaaggat gcgcaggcca
    27601 aatttatcct gccaaagctg tttggtactt tcttgtagtt tttctgcgcc tgccaccaca
    27661 tagcgtagac gatagaagtc atacgggttg gcgaaacgcg cgtagtgacc gaggaaagtc
    27721 gaggtaccga acaacacggt gcaactgcgg tcatacacca actccggcac aatgcggtaa
    27781 tgcagcgggc ttggataaag gaacacttct gcacctgtaa gcagtggcgt aaacaggcct
    27841 accgtcagcc caaaggagtg aaacagcggt aatgccgaca taaagcgatc gttggtggtg
    27901 aagtcggcaa tcgttttaat ctgctcgacg ttcgccagaa tgcttttatg gctatggacg
    27961 acgccttttg gatggccttc agaaccggag gtaaagagga tcagcgcctc ttcttccggc
    28021 tgctgtttta cctgcgccag gcgcggcatc agcaaatgag cgaagatcca tactttgtcg
    28081 gcagtggtga catctgcttt taaatcttcc agatagaccc agcgcacctg agtaagttgc
    28141 tccggcagat gccagagttt gcctttatcg agaaactggc gggaagtgaa gatggttttg
    28201 atttcagccg ccgtaatagc actggtcagc ccttttaccc cagcagtgta gttcatcatt
    28261 gcgggaatgc ggcgacgcgc gatggcccca aaaatcactg ccgcactgat gcctgcattg
    28321 ggcagcatta agccgatgcg ttcgccttca acactgtatt tttcaaggat gcgtccaaca
    28381 aacagcgttt tcgtaagcaa tttgcgatag gagtctgggg taaagttgac gtcttcgaca
    28441 catttcttcc cggctccgaa gcggtacatt gcactcagta aagattcgta cagagtttca
    28501 cgcgggcgca ccgccatacg cgcttccatc attatttgat gcagcatttc gccagcgatt
    28561 ttgcgacggt cacgggcacg cggcgcatcc ggcatcgcca cctgcgttgg tggcaaaata
    28621 tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac ctttcaggcg gctgaagtgc
    28681 gtaagttccg ccccttcaat acgcacagga ataaccgttg caccagactt cgccgcgaca
    28741 aaacccgcgc catcgtagat tttcatcagc gagcctgtag tggtgatgcg tccttcaggg
    28801 aaaatcacca ctggtcggcc ctgttccacc agacgtacca gatgtttaat agccataggt
    28861 tgcgtcgggt cgagaggaac aaagtcgata aatgatttca gccaacgcat ataccactgt
    28921 tggcttattg aggtgtaaac ggcaaacact ggacgcacag gtaaaaacag tccaagcaaa
    28981 atgccatcaa taaaagagac gtgattaggc gtaattagaa cgcgctcgcc cttcagtgcc
    29041 tgggtgtcac ccgtaacgcg aacgcgatac aaaacacggc acaaatttcg aaaaaagcta
    29101 aaaagcattc caactccctt tgctctgatt cagtaaaagc gaatggaggg agattacacg
    29161 agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa aaacctgcgc atccgcgcag
    29221 gttggtgcaa gagacagggt acgaagagcg taccgaataa tctcaccaat caatacctct
    29281 gggatcttga ttgtggtctg cacgacgact cttcgccagc gagaaaacgc aaaggaatga
    29341 agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt acagattgat gaccggcaaa
    29401 aaaaaacctg cgcatctgcg caggctggtg taattcatgt gctcaacccg aagttgactt
    29461 cacctatcaa tacctctggg atcaccactt tagcaacctg aagccaaacg ccaccagcgg
    29521 tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt tttttgtctt
    29581 acttatttgc gtttggctcg cattcccacg atgaaaacac gccacccctt gaaccaacgg
    29641 gcgttttccg taacactgaa agaatgtaag cgtttaccta ctaaggtatt ttcatggcga
    29701 ccataaagga tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc cgcgtcatta
    29761 ataattcacc taaagccagt gaagcttccc ggctggctgt gcatagtgca atggagtctc
    29821 ttagctatca cccgaacgcc aacgcccgtg cgctggcgca gcagaccact gaaacggtcg
    29881 gtctgatcgt tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa gcggtcgaac
    29941 aggtggctta tcacaccggt aatttcttat tgattggcaa cggttaccac aacgaacaaa
    30001 aagagcgtca ggctattgag caactgatcc gccatcgctg tgctgcgttg gtcgtccatg
    30061 ccaaaatgat cccggatgcc gatttagcct cattaatgaa acaaatgccc ggtatggtgc
    30121 tgatcaaccg tatcctgcct ggctttgaaa accgttgtat tgctcttgac gatcgttacg
    30181 gtgcctggct ggcaacgcgt catttaattc agcaaggtca tacccgcatt ggttatctgt
    30241 gctctaacca ctctatttct gacgccgaag atcgtctgca agggtattac gatgcccttg
    30301 ctgaaagtgg tattgcggcc aatgaccggc tggtgacatt tggcgaacca gacgaaagcg
    30361 gcggcgaaca ggcaatgacc gagcttttgg gacgaggaag aaatttcact gcggtagcct
    30421 gttataacga ttcaatggcg gcgggtgcga tgggcgttct caatgataat ggtattgatg
    30481 taccgggtga gatttcgtta attggctttg atgatgtgct ggtgtcacgc tatgtgcgtc
    30541 cgcgcctgac caccgtgcgt tacccaatcg tgacgatggc gacccaggct gccgaactgg
    30601 ctttggcgct ggcggataat cgccctctcc cggaaatcac taatgtcttt agtccgacgc
    30661 tggtacgtcg tcattcagtg tcaactccgt cgctggaggc aagtcatcat gcaaccagcg
    30721 actaaccgca gttaaagcaa ttccagcgcc agtaattctt cgatggtctg gcgacggcga
    30781 atcaaccgcg cctgaccatt atcaaacaga acttctggta acagcggacg gctattgtag
    30841 ttggatgaca ttgatgcgcc atatgcccct gtatcatgca gtaccagata atcacccgcc
    30901 ttcacttccg gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt aaagacatcg
    30961 cccgattcac ataatggccc ggcgacgacg gtttccaccg ttggcgcgtg ttccagagaa
    31021 cgaccatcag ctgccagggc actgatatgg tggtaactac cgtacattgc cgggcgcatc
    31081 agatcgttga acccggcatc aaccagcaca aagtggcggc tacccatttg tttgacgctc
    31141 cgcacctgag taattaacac gccagactgc gctaccagaa agcgacccgg ttcaatttcc
    31201 agtttcacag ggtggcccaa atggcgagcg atttgctcac gcgcggcatt ccacagacca
    31261 taataatgtt cggtatcaac cgcctcttca ccctgttgat aaggaataga aagcccaccg
    31321 cccgcagaaa tagcctgtaa atcctgaccg aattcgatga cctgacgtac catagcacca
    31381 cacacctgtt ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg aatgccgacc
    31441 agctgcaaat gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt gtaccagata
    31501 ccgtgcttgc tgttttcgcc accggtattg gttttttggc tatgtccgtg accaaacccc
    31561 gggttaacgc gcagccatac ccgatgccct ggcgaaacct ggcccagttg gtcgagcata
    31621 tcaacagaac ccgcattcac cggaatttgc aattcactga cgcgttcaag cgtcgcctga
    31681 tcgataacat ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt gtaacccgcc
    31741 gccaacgcac gctctatttc gcctaacgag acggaatcca ctttcacgcc ctgctcacgc
    31801 attaatcgca aaatatgaat attggaacag gctttctgtg caaagcgcac cacatcaaac
    31861 tgtttcagcg ctgcaatctg ccgacgaata atttgcgcat cgtagaccca caccgggcag
    31921 ccaaattcag cgggcaaacg cagcagattt tcggcggtga gatcggtatc ggtgctgaac
    31981 agtgaatgtg gcataacaaa ctccagatga gtgctttttt atgattacgc cacatcataa
    32041 aaagaataaa aaatatcgat ttatgtcgag tctatgcaaa aatgatatgg attaccggat
    32101 tgcgagagag cgctaatggc cgccgttaac ttacgtcata ttgaaatttt tcatgcggta
    32161 atgaccgccg gaagcctgac tgaggcagca cacctgctac acacctcaca gccaaccgtc
    32221 agccgcgaac tggcgcgctt tgagaaggtg atcgggctaa aattgtttga gcgcgtacgt
    32281 ggacgattac atcctaccgt gcaaggactg cgtctgtttg aagaagtgca acgatcctgg
    32341 tacggactgg atcgcattgt cagcgccgca gaaagtctgc gcgagtttcg ccagggagaa
    32401 ctgtctattg cctgcctgcc ggtcttttcg caatcttttt taccgcagct cctgcaaccc
    32461 tttctggcac gttatcccga tgtcagctta aatatcgtgc cccaggaatc accgctactt
    32521 gaagagtggc tctcggccca gcgtcatgat ttaggactca ctgagacact ccatacgcct
    32581 gcgggaacag aacgtaccga attactctct ttagatgaag tgtgtgtgtt acctccgggt
    32641 catccgctgg cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga gaactacatc
    32701 agcctttccc gtactgacag ctatcgccag ttgctggatc agctatttac tgaacatcag
    32761 gttaaacgac gcatgatcgt agaaacccac agcgccgcgt cagtctgcgc aatggtacgg
    32821 gcgggggtag gtgtttcggt ggttaacccg ctcaccgcac tggattatgc ggcaagcggt
    32881 ttagtggtac ggcggttcag tattgcggtt ccgttcaccg tcagcctgat ccgccccctg
    32941 caccgcccgt catcagcgct ggttcaggcg tttagtgagc atttacaagc ggggttaccg
    33001 aaactggtca cttctcttga cgcgattttg tcgtcagcta cgacagcata aaagcgacag
    33061 catcctcggc atggatcgcc gcagtatcaa acacaggcaa aacactgcgc tcttctggaa
    33121 ccaataaacc aatttctgtg cagccaaaaa tgacaccctg cgccccctgt tctgcaaggc
    33181 gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt aaattgcccc agacacagtt
    33241 cttcaaaaat aatctgatta attttcgccc gttcatccgc ttcaggaata agacaattga
    33301 tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg taacgcgtac
    33361 ccagcagcgc cacacgagtc attccggccc cggtaattgc acgtccggtg gcatccgcaa
    33421 tgtgtaagaa aggcagagtg caacgtgact caatggcatc cgccacttta tgcatcgtat
    33481 tggtacataa cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc gcctcagcca
    33541 gaatgtcccc ggttttatcc cattccccgc gacgctggca ctcttctatt tcatgaaaat
    33601 cgacgctatg tagcagcact tgcgcagagt gaagcccacc aagccgctgt ttaatgcctt
    33661 catttatcag acgatagtaa ggaatggtgg attcccagct cattcctccc agcaaaccaa
    33721 ttgttttcat tcctctctcc tgatgtgtgt taccgcaaca aggctgaata aggaacgaga
    33781 caaacgcctc aacggccagg tgcccgatct ctattaacga aaaaagggcc ggatgtacag
    33841 cacatccggc ccgtgaaatc agacgccgat atttctcaac ttctcgcctg ccatcagttt
    33901 gcgttcgata tgttccagcg tgacattttt ggtttccgga atgagccaga aagtgatgcc
    33961 cacaaacgca atgttcagcg cagtgtagag ccagaacgta ccggcagcgc caatgctatc
    34021 aagcagtgtc aggaaggtcg cgccgataat catattcgac acccagtttg tcgtcgtcga
    34081 acaggtaata ccgaaatcgc ggcatttcag cggctgaatt tcagagcaca ggatccacac
    34141 cactggcgcg gcgctcatcg cataaccggc aatacacatc atcgtcatgc caacagagag
    34201 ccaggacaag ccactggaag ccgtaccgtt atcaaactgc atcaggcaat agcccagcac
    34261 cagagtgcct aacgccatca cgctgaaacc aattttcaga gccggtttac gacctgcttt
    34321 atctaccgta aagaccgcaa tgaaggtcgc gaacataaag gtcagtccca cgaccagagt
    34381 cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt ttgaagatac gcggcgcata
    34441 gtacatgatg atgttcatac cggtaaactg ctgcatcgcc tgcaacaaca taccgagaaa
    34501 cacagcacga cggacgttac ggttgatctt aaacagtgcc caaccgcctt gttttaactt
    34561 caggctttca cgaatttcgt tgagttcttc tcgcgctttt tccgacgtat cgcgcagcat
    34621 acgcaacact tcttccgcct caatatgacg ccccttttcc gccagccagc gcgggctatt
    34681 tggcaggaag accaccagaa taatcagcag gactgctggt aaagcaagaa cccccaacat
    34741 tgcgcgccag ttaccgctat aactgaacgc tgtatcggat aaaaacgcca gcacgatgcc
    34801 gagtgtgacc atcaactggt acatactgat catcttaccg cgaacgtttt cacttgccat
    34861 ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac
    34921 acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag aaccgagtac
    34981 aaacaggatg gcccccgcca tcaggctgta tttacgaccc aggcggaacg acagccaacc
    35041 attaaacagc gcaccaattg ctgcgccgag catcatgcta ctaaccaccc attcctgcaa
    35101 acgactggtc agcacaaagt gatcggtaat gaacggcaac gctccggcga ttacgccgat
    35161 atcaagacca aataacaatc ctgcgaccgc agcagctacc gaaacaaaca tattcatacg
    35221 ccgcgtatcc cgcaaagaac gtggcgttaa agcagattcc gtattgatag taaccatttt
    35281 ttcctgccag cagagagtaa gacatagtga aaaaatacgt gaacaactca cgcaggtgtc
    35341 aggtcggaaa cagcataaat atggattaaa ttgctgcgac atgtcgttat gtgatggata
    35401 ttccaatttt caaattaagt tgaattattg agattattat taaccaccta attttacagc
    35461 agataaaatt cataaagttc attaattgat aattaatatg gattatttca taaccatgat
    35521 atggattatg atgatctaca ggtataaaaa accctgccat gcggcagggt cataaaagta
    35581 agaagaatga attaacgcgc cagccaaccg ccatccacgg caatggtata accattcaca
    35641 taatctgaag cgctggaggc aaggaacact atcggcccca tcaggtcact cggcagtccc
    35701 caacgaccag ctggaatgcg gtcgagaatt tccgcgctac gttgttcatc tgcccgtagt
    35761 tgttgagtat tgttggtcgc catgtaaccc ggagctatcg cattaacatt aatgttgtgt
    35821 ttagcccatt cgttcgccat caatcgcgtc acacccatca cgccgctttt tgatgcggta
    35881 taagaaggca cacggatccc gccctggaag gagagcattg acgcgatatt gataatcttg
    35941 ccgccattgc cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa gaatacgctc
    36001 ttgatattca ggttcatgac atcgtcccag tccttttcgc tgaactcgag agcatcttcg
    36061 cggcgaatca atccggcgtt attcaccagg atatcaatat gaccaaactc cgctaccgcg
    36121 cgatccagca gtccaggaat gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa
    36181 cgacgcccca gcgccgtgac ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca
    36241 acaatgtcac agcccgcttg cgccagcccc aacgccatcc cctggcccag cccagtatca
    36301 caaccagtga cgaccgcaac tttaccttcg agagaaaatg cacttaaaat cataacaata
    36361 ccttattcgt ttatgcccac aactagcgca aatctttaac ggccacatgg tccatatcat
    36421 caaagacctg gttttcaccg accatgcccc agataaaggt ataagctttg gttccgacgc
    36481 cggaatgaat cgaccagctc ggggagatca ccgcctgctc gttatgcatc acaatgtgac
    36541 gcgtttcttg cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc atattgaaat
    36601 agaaataaac ttccatccgg cgctcgtgag tgtgacacgg catggtgttc cacaagttac
    36661 ctggagccag ctccgtcagc cccatactca attggcaggt ttccagcaca tccgggacaa
    36721 aatatttgtt aattgtgcga cggttactgg tgaggttatc gcctaacgtg actggagata
    36781 cttcgtccgg tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat
    36841 aaaactttgc cggagtggcg gtatcaatac tggcaaagac aacttctttt gcacctttac
    36901 ccacatacag ggcgtcgcgg tgaccgattt catagcattg gccatcgaca gtaatcgtac
    36961 cggcaccgcc aatattgata acacctaact cgcgacgttc aaggaaatag cttacgccga
    37021 gttgtttacc aacttccccg ccaacggaaa ccgttttagt tatcggcata atgccgccaa
    37081 caataattcg gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca aatacctctt
    37141 caaccaaaaa ttcattgcgc agcccttggg tatccagcgt ttttgcgtgc gcactgtgga
    37201 tgctctgtct tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg
    37261 atagtgaaac actgtttcgt tttctctata aagggatgat atgcccatgg gctagggttt
    37321 tcaattaaaa ataaaacaat gtttcatttt taagttaaga attaaaaaaa gtgcagtgtc
    37381 gatcacgaat atgtcatgag aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt
    37441 gccagccatc attattcaac agattaatga cggcagatat ttatcggtaa ggaggaaatg
    37501 cagagctact cgtcacgttc aatggtcaat gccacgccct ggcccccgcc gatacaaagc
    37561 gttgccagcc ctttgcgggc attacgtttc accatttcat gaaccagaga aaccaggatt
    37621 cggcaaccgg aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt gacattgacc
    37681 cgacgctcat cccactcaag catcttgcca accgaaagcg cctgtgcagc aaacgcttca
    37741 ttagcctcga taagatcgac atcagccaac tgccagccta cacgctccag gcaacggcgg
    37801 gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct ggcaaatgcg
    37861 cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct catcatcatt
    37921 accgcagctg cgccatcgtt tatggatgat gcattccccg cggtcacaga accgagacta
    37981 tcaaatgaag gatttaaacg ggctaaacct tctgcactgg cgtcagtgcg tggctgttca
    38041 tcggtatcaa caaccaacgt ctgaccgtta ctttggatca ttaccgggac gatctcatct
    38101 ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca
    38161 tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt gacaccaata
    38221 tgataatcat tgaaggcatc ccacaaccca tcatgcacca gactgtcaac caactggcta
    38281 ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg gctcatgttt
    38341 tcctggccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg agtagccaga
    38401 tgcagtgctt taagcccaga accgcaaacg tcattaatag tgattgcaga aacgctatta
    38461 ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc tgcagtcaac
    38521 acctgaccaa gaattacttc atccaccgca tatgcaggaa cgccggtacg ttctattaac
    38581 gctttcacga ccagactacc aagttccacg gcggaatgac ccgctaacgc accgcgaaag
    38641 cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac aacctcgcac
    38701 caattaacag tgcaatagta aatgattgtt aattacagtt atcttaattg tttaaaaaaa
    38761 gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa ctggcacgta
    38821 aggtttgcaa ccactaaccc accaatagag gggtagatag ggcgttaatc tcccatactt
    38881 aacctggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat acgttcagat
    38941 aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct acggcacagc
    39001 cgttggtgca ggcacgctct tccttcctat tcagttaggt tcggcagggg ctgtggtcct
    39061 gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag ccttatgcca
    39121 gttcatcctc tcatcgaaaa catcagcagg tgaagggata acgggcgcag taacacacta
    39181 ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct tttttgtcgt
    39241 cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg caaagcatat
    39301 ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa tcctgaatct
    39361 catttttctg atgggacgcc atgcaaccat tcgggtaatg ggttttttgg tattcccatt
    39421 gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac ctgatctatt
    39481 aacgacccag gtagagttca atcagaatac ccttcaccag atatggatat cgattcccgt
    39541 gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag acagacgtga
    39601 aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg cttatctcat
    39661 catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc caccttcgta
    39721 tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta tgctgccgaa
    39781 cgccccagca tggctgtcaa tttccgggat tattgtcgca gtagttgcga tgtcgaaatc
    39841 attcctgggt acgtactttg gcgttattga aggtgccacg gaggtcgtca aaacaacact
    39901 acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt
    39961 atcgctgatc accttcattg tttgttgcat taacccgaac gcgatttcga tgatttacgc
    40021 gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgttgt caacgtatct
    40081 gatcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga tcgtgggtat
    40141 cctgtgcgta tcggtaatgt tctttagctg atatccattg tgaatggttc aaaattgcta
    40201 caattttgaa ccattccagt tttcataatg ttcattcgtt attattccgc actatcactt
    40261 ccctcatcat atcctagtat tatcagttag ttaaacacac aaagagatct gaacatctta
    40321 aataagacta tttaagatgc ataacttaga ttcgcaagat atcttccggt cattcaaaaa
    40381 caatttgcga tatagatatt ctcataccat gagatagtta tctgaaccac cctcaaccag
    40441 gtaag
//
LOCUS       DAZFDP010000026        37827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFDP010000026 DAZFDP010000000
VERSION     DAZFDP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            221..358
                     /locus_tag="ACHJ1G_001489"
                     /pseudo
     CDS             221..358
                     /locus_tag="ACHJ1G_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(466..1701)
                     /gene="aslB"
                     /locus_tag="ACHJ1G_001490"
     CDS             complement(466..1701)
                     /gene="aslB"
                     /locus_tag="ACHJ1G_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4763372.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1848..1924)
                     /locus_tag="ACHJ1G_001491"
     tRNA            complement(1848..1924)
                     /locus_tag="ACHJ1G_001491"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1888..1890),aa:Pro,seq:tgg)
     gene            complement(1967..2053)
                     /locus_tag="ACHJ1G_001492"
     tRNA            complement(1967..2053)
                     /locus_tag="ACHJ1G_001492"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2017..2019),aa:Leu,seq:cag)
     gene            complement(2074..2149)
                     /locus_tag="ACHJ1G_001493"
     tRNA            complement(2074..2149)
                     /locus_tag="ACHJ1G_001493"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2114..2116),aa:His,seq:gtg)
     gene            complement(2208..2284)
                     /locus_tag="ACHJ1G_001494"
     tRNA            complement(2208..2284)
                     /locus_tag="ACHJ1G_001494"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2248..2250),aa:Arg,seq:ccg)
     gene            complement(2387..3772)
                     /gene="yifK"
                     /locus_tag="ACHJ1G_001495"
     CDS             complement(2387..3772)
                     /gene="yifK"
                     /locus_tag="ACHJ1G_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4763373.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3963..4703)
                     /gene="wecG"
                     /locus_tag="ACHJ1G_001496"
     CDS             complement(3963..4703)
                     /gene="wecG"
                     /locus_tag="ACHJ1G_001496"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4763374.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4706..6058)
                     /gene="wzyE"
                     /locus_tag="ACHJ1G_001497"
     CDS             complement(4706..6058)
                     /gene="wzyE"
                     /locus_tag="ACHJ1G_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4763375.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6055..7134)
                     /gene="rffT"
                     /locus_tag="ACHJ1G_001498"
                     /gene_synonym="wecF"
     CDS             complement(6055..7134)
                     /gene="rffT"
                     /locus_tag="ACHJ1G_001498"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4763376.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7131..8381)
                     /gene="wzxE"
                     /locus_tag="ACHJ1G_001499"
     CDS             complement(7131..8381)
                     /gene="wzxE"
                     /locus_tag="ACHJ1G_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4763377.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8383..9513)
                     /gene="rffA"
                     /locus_tag="ACHJ1G_001500"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8383..9513)
                     /gene="rffA"
                     /locus_tag="ACHJ1G_001500"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4763378.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9518..10192)
                     /gene="rffC"
                     /locus_tag="ACHJ1G_001501"
                     /gene_synonym="wecD"
     CDS             complement(9518..10192)
                     /gene="rffC"
                     /locus_tag="ACHJ1G_001501"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4763379.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10170..11051)
                     /gene="rfbA"
                     /locus_tag="ACHJ1G_001502"
     CDS             complement(10170..11051)
                     /gene="rfbA"
                     /locus_tag="ACHJ1G_001502"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4763380.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11070..12137)
                     /gene="rffG"
                     /locus_tag="ACHJ1G_001503"
     CDS             complement(11070..12137)
                     /gene="rffG"
                     /locus_tag="ACHJ1G_001503"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4763381.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12137..13399)
                     /gene="wecC"
                     /locus_tag="ACHJ1G_001504"
     CDS             complement(12137..13399)
                     /gene="wecC"
                     /locus_tag="ACHJ1G_001504"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4763382.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13396..14526)
                     /gene="wecB"
                     /locus_tag="ACHJ1G_001505"
     CDS             complement(13396..14526)
                     /gene="wecB"
                     /locus_tag="ACHJ1G_001505"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4763383.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14582..15628)
                     /gene="wzzE"
                     /locus_tag="ACHJ1G_001506"
     CDS             complement(14582..15628)
                     /gene="wzzE"
                     /locus_tag="ACHJ1G_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4763384.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15640..16743)
                     /gene="wecA"
                     /locus_tag="ACHJ1G_001507"
     CDS             complement(15640..16743)
                     /gene="wecA"
                     /locus_tag="ACHJ1G_001507"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4763385.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16983..18242)
                     /gene="rho"
                     /locus_tag="ACHJ1G_001508"
     CDS             complement(16983..18242)
                     /gene="rho"
                     /locus_tag="ACHJ1G_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4763386.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18327..18428)
                     /gene="rhoL"
                     /locus_tag="ACHJ1G_001509"
     CDS             complement(18327..18428)
                     /gene="rhoL"
                     /locus_tag="ACHJ1G_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4763387.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18569..18898)
                     /gene="trxA"
                     /locus_tag="ACHJ1G_001510"
     CDS             complement(18569..18898)
                     /gene="trxA"
                     /locus_tag="ACHJ1G_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4763388.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19029..20294
                     /gene="rhlB"
                     /locus_tag="ACHJ1G_001511"
     CDS             19029..20294
                     /gene="rhlB"
                     /locus_tag="ACHJ1G_001511"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4763389.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20428..21912
                     /gene="gppA"
                     /locus_tag="ACHJ1G_001512"
     CDS             20428..21912
                     /gene="gppA"
                     /locus_tag="ACHJ1G_001512"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4763390.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21959..23980)
                     /gene="rep"
                     /locus_tag="ACHJ1G_001513"
     CDS             complement(21959..23980)
                     /gene="rep"
                     /locus_tag="ACHJ1G_001513"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4763391.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24197..24646
                     /locus_tag="ACHJ1G_001514"
     CDS             24197..24646
                     /locus_tag="ACHJ1G_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4763392.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24845..25126
                     /gene="ppiC"
                     /locus_tag="ACHJ1G_001515"
     CDS             24845..25126
                     /gene="ppiC"
                     /locus_tag="ACHJ1G_001515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4763393.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25213..26688)
                     /gene="ilvC"
                     /locus_tag="ACHJ1G_001516"
     CDS             complement(25213..26688)
                     /gene="ilvC"
                     /locus_tag="ACHJ1G_001516"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4763394.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26838..27731
                     /gene="ilvY"
                     /locus_tag="ACHJ1G_001517"
     CDS             26838..27731
                     /gene="ilvY"
                     /locus_tag="ACHJ1G_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4763395.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27783..29327)
                     /gene="ilvA"
                     /locus_tag="ACHJ1G_001518"
     CDS             complement(27783..29327)
                     /gene="ilvA"
                     /locus_tag="ACHJ1G_001518"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4763396.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29330..31180)
                     /gene="ilvD"
                     /locus_tag="ACHJ1G_001519"
     CDS             complement(29330..31180)
                     /gene="ilvD"
                     /locus_tag="ACHJ1G_001519"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4763397.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31245..32174)
                     /gene="ilvE"
                     /locus_tag="ACHJ1G_001520"
     CDS             complement(31245..32174)
                     /gene="ilvE"
                     /locus_tag="ACHJ1G_001520"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4763398.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32194..32457)
                     /gene="ilvM"
                     /locus_tag="ACHJ1G_001521"
     CDS             complement(32194..32457)
                     /gene="ilvM"
                     /locus_tag="ACHJ1G_001521"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4763399.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32454..34100)
                     /gene="ilvG"
                     /locus_tag="ACHJ1G_001522"
     CDS             complement(32454..34100)
                     /gene="ilvG"
                     /locus_tag="ACHJ1G_001522"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4763400.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34103..34153)
                     /gene="ilvX"
                     /locus_tag="ACHJ1G_001523"
     CDS             complement(34103..34153)
                     /gene="ilvX"
                     /locus_tag="ACHJ1G_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4763401.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34240..34338)
                     /gene="ilvL"
                     /locus_tag="ACHJ1G_001524"
     CDS             complement(34240..34338)
                     /gene="ilvL"
                     /locus_tag="ACHJ1G_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4763402.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            34691..36211
                     /gene="yifB"
                     /locus_tag="ACHJ1G_001525"
     CDS             34691..36211
                     /gene="yifB"
                     /locus_tag="ACHJ1G_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO4763403.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36236..36574)
                     /gene="maoP"
                     /locus_tag="ACHJ1G_001526"
     CDS             complement(36236..36574)
                     /gene="maoP"
                     /locus_tag="ACHJ1G_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4763404.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36693..37532
                     /gene="hdfR"
                     /locus_tag="ACHJ1G_001527"
     CDS             36693..37532
                     /gene="hdfR"
                     /locus_tag="ACHJ1G_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4763405.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37628..37703)
                     /locus_tag="ACHJ1G_001528"
     tRNA            complement(37628..37703)
                     /locus_tag="ACHJ1G_001528"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37668..37670),aa:Trp,seq:cca)
     gene            complement(37712..37788)
                     /locus_tag="ACHJ1G_001529"
     tRNA            complement(37712..37788)
                     /locus_tag="ACHJ1G_001529"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37752..37754),aa:Asp,seq:gtc)
BASE COUNT         9260 a        10305 c         9501 g         8761 t
ORIGIN      
        1 atcaataatc aacgcgatat aataaacctg ccttacatat caactgcgcc agaggtagga
       61 ttgaaaacgc tctcctgatt ttccaattca ttttctggat aaataaataa tttatttttg
      121 tcactattat ttatgtaatc atcctgtcag ggagagggat ctcaattatc aatgcttaat
      181 tacgtcatca ttttgatgac atgaaaccgg agcattttgc atggaatttt cgttttcacc
      241 caaacttctt gttgttgctg tcgccgccgc tcttcctctc atggccagcg cagcagatac
      301 cccgtcaact gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc
      361 acagaacgct gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc
      421 cgcatccggc aatcaaccgc aggcggccac aaaatggccg cttttttact tactcaccac
      481 cagcaaatgc gcatgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat
      541 tgctttaaga tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt
      601 cagccccggt ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccagca
      661 cgcttttaac acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc
      721 aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc
      781 cagccgatat tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac
      841 aatcaccgag cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa
      901 attggcaaac gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca
      961 gtggtcgaat atcgccacca gaaattcacc aaattcatcg gggcgcaccg accattccgt
     1021 cagttccccc tgaatatcac caggcgcatg tagtttcagt ccatcgctgg cagctgtttc
     1081 atcagccagg cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa
     1141 aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt
     1201 atagtcgaca tgatgttttt gcagcagcgt cagggcacgc atcaccagct tatgcgttgg
     1261 tctgccacct ttggtcacgc gatattgatt gtggatctca gccggaccgt ccagcgataa
     1321 cccaacaaga aaatgatttt ctgccagaaa tgcacaccat ttatcatcga gcagcacgcc
     1381 gttagtctgg aagctgttac ttatcttcct gccagcaccg tatttcgcct gtaactttac
     1441 ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg ccgccctgcc aggtaaaagc
     1501 cacttcgttt tgcggttcgc tggcagcgat atagtgacgg acatacgctt ccagcgtgtc
     1561 atcgtccata tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca
     1621 gtagtcacag ttcagattac aatcggaacc actcggtttc gccatcacat gaaaagcacg
     1681 cgttggaacc tgttgcagca tggtcactcc ttgcctgaag tcatccttca atacgttaag
     1741 ggcgaaatca taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga
     1801 cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag
     1861 aggattcgaa cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc
     1921 gccgaaatac tactttttga atttttagtt caattcttta aagtcgtggt gcgagggggg
     1981 ggacttgaac ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt
     2041 ccgccacctt cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca
     2101 cgacaactgg aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc
     2161 ttgttacgcg gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac
     2221 aggattcgaa cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg
     2281 gcgcttagcg ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct
     2341 ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt tgctgattat tcctccagtt
     2401 tatgcgcttt cccgtggcga ttaaggccaa aaactttata aatcgccgtc accgccagca
     2461 taaagatgat gccaacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca
     2521 aaacgcaaat caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga
     2581 acggatggct ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata
     2641 tcacaaacca tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac
     2701 gctgcggatt gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata
     2761 ctgccacacc cgcaactggt acgccgtgac gggaaacttt cgccatcgcc gccggtaact
     2821 gacggttttt cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc
     2881 cagagagcgc agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga
     2941 ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga
     3001 tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc
     3061 ctacggcact gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca
     3121 gctccacgcc ctggtaggac gccaccacaa tacacagcgc ggtcaggaac cctttccagc
     3181 cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat
     3241 tgccaaagcc aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga
     3301 ctttgatcat cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg
     3361 ccaacgccac cagcgccact gcgatcaatg cgggtatcca ctgcgccatc tccgggaacc
     3421 agaactggac ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa
     3481 accagtaaga ccaggcggtg agataaccaa agaacgggct catataacga tgcgcataaa
     3541 cggcgaacga accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga
     3601 aaaagacgaa tagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca
     3661 aggtactggc ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt
     3721 cgatatgtcg agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc
     3781 gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg
     3841 cgaatgtttt cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg
     3901 tgtaataaaa aagcagacag gcgacggagt gaccactccg tcgctttaca aagagaggaa
     3961 aatcagaggt tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta
     4021 atgcggctcg gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt
     4081 ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac
     4141 agcgcatctg gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc
     4201 atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc
     4261 tgctcgggtt taaaataacc atcctgactg ccaacgatat tcacattcca ctggttgcgc
     4321 agtttagctt cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc
     4381 ccttctttgc ctgcgcgcgc catcagctct tcccagagat cagcaccggc aacgcgggaa
     4441 acctgcgcct gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat
     4501 ttaaattcgg cagcgttaat tagctccctg acctcagcat tatcttcaat ggtcagcatt
     4561 ttttcggcat taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa
     4621 tcgagggcgt gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc
     4681 ggtgccatgg tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg
     4741 tacgtttatg aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca
     4801 gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt
     4861 cacgtgccag cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag
     4921 ccttatagcg attaggctcg cggttgccca gctcatacag ccagtcgaac catttgatga
     4981 tcagaccaac cacgatcgcc ccgagcggga tgaacaacgc gccgcccatc accaccagtg
     5041 agcctataag cgtaggcgag atcgccagtc cggagtggtt attcagcact tcccaggtaa
     5101 agtagttggc tgagttcagc accatactcg ggcgacccgg ccacagccag gaagggataa
     5161 agacatagaa atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct
     5221 gcaacagcaa cgccagattc tcccacggcg agaaggtgtc gcgagtgaga tagagaaacg
     5281 tatagaacgc ttcatcgccg ctcacattca ttccatagcg ttttagcgcc agccagaaca
     5341 tgcccacaat ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa
     5401 taatgccaat aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga
     5461 caatcatata agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt
     5521 tgctatcctg gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc
     5581 gttttaacgc cacgccggag acttcactgg agaagatctg actgtaggag ttaagccgga
     5641 acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atgcccatca
     5701 ggatcaccca cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta
     5761 catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc
     5821 agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc
     5881 gaaataccag cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg
     5941 aaaagaagac attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa
     6001 taaacagcgt gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc
     6061 gccctccccg gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa
     6121 gaaggcaatg gtgtttttat ccaccgacgc caactggcgc tgcgcttcac gcacaatatc
     6181 ctcgttgaga tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca
     6241 gaacggattt tcccggttaa gcacacaagg aatgcccgcc tgaatcagta agcacagcgt
     6301 accaatgccc tgctggcgag caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc
     6361 cagataggcg tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa
     6421 ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat
     6481 cggcaccacc acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac
     6541 atgctcattg ctgaggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg
     6601 ccgatcgttc gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc
     6661 tccccgcacc tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac
     6721 acagccgact cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc
     6781 actggaaagc tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat
     6841 gccaccactc agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg
     6901 ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg
     6961 gaaaaattgc acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac
     7021 cataaactcg cgcgcgtgct cgctcgtcgc agccagcgcg tcattgaaaa accgcaaaac
     7081 ggttcggtta tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc
     7141 ctacgccaga gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata
     7201 tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc
     7261 aataaagtga actggctgat ttccgccaga atataaaacc gcaatgacgc tttggcgatc
     7321 accagataac caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca
     7381 aagagatcgc gcatagcggt aaatttattc gacaacagca gccagatagc aaaatcacgc
     7441 agcagccaga cggtaaaact cgccgccgcc actgccggta agacgaattt cagcgattta
     7501 accacttccc gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat
     7561 acgctgaacg atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc
     7621 cagatcccca cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa
     7681 gcaacaggca aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct
     7741 gccagaccgt tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttgatc
     7801 aacatgatag tggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc
     7861 ccttcataac cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg
     7921 acaatcagcg ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac
     7981 aggttgcccc aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag
     8041 tcggtattac caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc
     8101 atcagcgtag agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt
     8161 tgcggattat catggtactg ggcaacgtat ttggttacac cgttaaagat gccagctccg
     8221 gcaagcacgc cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc
     8281 ggaccaaatg acacagccag caacttaccg accagtaacc cggcaccaat cttgaccagt
     8341 gtactggccg ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa
     8401 caaagtcgca attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg
     8461 cagcaggcgc tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagcg
     8521 ttcccccgca gggcaaccgt gcagcggaat gtaatggaac accgccatga tttccgcttc
     8581 tttcagaaag ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat
     8641 atgcgcgttc tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc
     8701 cagaggcgct aacgcatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg
     8761 atccgctgct tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct
     8821 ggagccaata tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt
     8881 agtgcccttt tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt
     8941 cgcgccgcct tcaccacccg ccgtatagtt tttggtttca tggaagctaa agcagccaat
     9001 atgaccaatg gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc
     9061 ttctaccaca aacagattat gctttttcgc caacgccata atggtgtcca tttcgcaggc
     9121 cacacccgcg taatggaccg gcacgataac gcgcgttttg tcggtgatcg ccgcttcaat
     9181 cagcgtttcg tcgatgttca tggtgtccgg gcgaacatcc acaaaaacga tttttgcgcc
     9241 acgcagcaca aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc
     9301 gccaggctgg atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt
     9361 cagtaacact ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa
     9421 accgccatcg ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt
     9481 tcccaccacc ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg
     9541 tgctttctac attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt
     9601 gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca
     9661 tcagctcagc acctacaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga
     9721 gttcccgtaa agagacataa ccgcgaatac cgccggacgc cgcacgtaaa atcagacatt
     9781 gatgatcaaa ggtgccgcgc acggcatttt caatccactg tgcataaaag cgtctgctgg
     9841 cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctggctaact
     9901 gacgtaatgc gggaatatcg gtctcttgtg ccactacagc accactatca ctgacattgt
     9961 tcacgggtag tgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg
    10021 catccagttc acccgtattt gatgccgcaa ttttggcctg cacccgtgac cacggcgcta
    10081 acacgtctgg cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac
    10141 caaagaaggc gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac
    10201 tccagcagat attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc
    10261 tcgtcatcga gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc
    10321 tggcgttttt ctactgtctg cacaaaggta ctggcttcaa tcaggctgtc gtgagtgcca
    10381 gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg
    10441 tacatctggt tgatggaggt aatctccagt tcaccacgct ccgacggctt cacctgcttt
    10501 gcgtactcca cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc
    10561 ggctgttttg gcttttcttc cagcgagata gcgcggaaat tgtcgtcaaa ctccaccacg
    10621 ccaaagcgtt ccgggtccat cacctgatag ccaaataccg tcgccccttc cgtgcgcgcc
    10681 gcaacatgac gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc
    10741 agacaagaag gttcaccgtt gaggaaggtt tcaccgatga taaaggcctg cgccagaccg
    10801 tccgggctgg gctgttcggc gtattccagc tggataccga actcactacc atcgcccagc
    10861 aggcgctgga aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg
    10921 gccaacatca gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt
    10981 tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc cggaaccgcc cgccaggata
    11041 atacctttca tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcgccctga
    11101 tagctgccgt cctgcacctg cttccaccag ctttcattag ccagatacca ctgcaccgtt
    11161 ttacgcattc cactttcaaa ggtttcctgt ggcagccaac caagttcacg ggcaattttc
    11221 gaagcatcaa tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcaag
    11281 tcacgatagt gcgccacgcc gtgcggcttg ttcggagcca gttcttccag cagctcgcaa
    11341 atggtttcca caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca
    11401 ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc
    11461 cagtcacgga tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg
    11521 aggatcatca gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag
    11581 ttggtgatca gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgcta
    11641 ctggcttttg acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa
    11701 tcatccgtcg agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac
    11761 gctgattttt tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta
    11821 taagtcccga caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca
    11881 tggctttcgg ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc
    11941 agttctgccc gatcgcagat atcaactttc tcaaaggcaa agtgctcgct ttgcgcgacc
    12001 ggtgccagcg acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg
    12061 ctcgtttcgt tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt
    12121 atcagaattt ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga
    12181 cattgtcgcc attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg
    12241 ccgttgccag cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca
    12301 actggtggat gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt
    12361 cagcgatttc catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac
    12421 caaagcaggc gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa
    12481 ccgccgcttt cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg
    12541 tacggataag ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa
    12601 tgcagtgacc gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg
    12661 ccaggcgaat cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat
    12721 tagcaaaagc gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg
    12781 tccgcgagtt agtgacgaca cactcacctt cgaggaaaat tttgtacagt tcgctggcgc
    12841 gggtcgaaca aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca
    12901 taacctggcc cggtaacacg cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc
    12961 ccacctgctg tgggaaagtg agatccggac gcatctctgc taaccattct gccatcttct
    13021 cggttgaccc caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg
    13081 gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa
    13141 acggcgtggg tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta
    13201 aaaaaccgcc ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga
    13261 tttcgccacg attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt
    13321 tttgccgtga ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag
    13381 aaatggtcgc aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga
    13441 gcatgcctga ccatcaccat acgggttatg ggcgcggctc atagtttgat attcgttttc
    13501 gtcttttaaa agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg
    13561 caccgtaccc gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg
    13621 tttccccagc gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc
    13681 gtggttcatc agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt
    13741 cacatgcccc agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata
    13801 gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc
    13861 aaagccacga ccgaaacttt cacgcctgtg accggtcacc agaatcatct ttttatcggg
    13921 gtcgataaac gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg
    13981 atcacgcacc cataacagtg catcaatgac tgtattacca gtaatgaaga ttcggctatc
    14041 cgcaacgttt tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat
    14101 cgccagatgc ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc
    14161 cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct
    14221 ggttgccagc gtcgttgttg tgtcgccgtg aaccagcacg acgtctggtt tgaactcggc
    14281 aagaataggt tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg
    14341 ctgcattatg ttgagatcgt agtcaggtac aatggaaaag agtttcagca cctgatcgag
    14401 catctcccga tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa
    14461 cgcatgcacc aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac
    14521 tttcacatcg attctcttcg aataagcggc gagcgccttt gcgctcgccg cagcagtgtt
    14581 gctatttcga gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc
    14641 cccacataat catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg
    14701 tacgcaaata gcgataggtc tgaaaacgcg gatccagagt tggaccaaca ttcagggtgt
    14761 ttaacatggc ccgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat
    14821 tttccagtcg agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt
    14881 cctcggcagg cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg
    14941 cctgctcaat gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct
    15001 tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcctcattca
    15061 gatggctggc tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt
    15121 caggcgcggt ttcggcaata agcttcacgc tgtcattgac cgcgcgggta aagtctccgg
    15181 ggataaactg gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc
    15241 ccaccatccg ctgcttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg
    15301 aggccagttg cataacaaac tctttatagg cttcgtccat gaccgatggt tggtcggcag
    15361 aagccatgtt tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc
    15421 ccagcatatt caccgttgga cgatcggtaa tcgccgtcgc gctccactct tgtcgagcaa
    15481 aaaaagtata cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct
    15541 tcccagccca caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg
    15601 ccggtttccc aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct
    15661 gccaccacga tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca
    15721 ggcacgctta atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac
    15781 ccactccggg acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc
    15841 ggcaagggta atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg
    15901 gtgaatatgc tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat
    15961 aatcgccacc atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat
    16021 gggatgggtt ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag
    16081 cgtactgccc gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag
    16141 catgatgtat ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg
    16201 cccgtcgaac cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag
    16261 caagccatca atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag
    16321 cgtcaggaag taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag
    16381 gctactgaga taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt
    16441 ggcacggatt tttacgctga tatcaaaacg gtcatccagc gcgccaatga aaacaagcac
    16501 accggcacaa gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt
    16561 gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg
    16621 tttgcggaag tttggtttat ccactaaacc gacttttttt gccactttac gggcaaaaaa
    16681 cagaaacagt gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt
    16741 cacaatgcat gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc
    16801 agaagggaaa gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc
    16861 aaattgcata acatgccagc aaattccagg aattttccta acatcgccag cgcggcatta
    16921 agattacaga tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa
    16981 atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg tcttagtcat ggccagttta
    17041 ttaatgagga attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc
    17101 cacattttct gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg
    17161 ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc
    17221 atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg
    17281 ataagcgccg tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcaccg
    17341 aagaagcgtt tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct
    17401 gacgccggaa caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg
    17461 ataacgtctt tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc
    17521 tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga
    17581 cgctgcatct cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa
    17641 tccgggtggt tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct
    17701 ttcggcggtg ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt
    17761 acgcgagcgg ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt
    17821 gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg
    17881 aagttaactt cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga
    17941 atcttaccag agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa
    18001 acgtagatgt catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat
    18061 ccatcctgca atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc
    18121 tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac gagccaggtt ttccagcccc
    18181 atattttcgc cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc
    18241 atagtggtgt gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg
    18301 gcaggatcat ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca
    18361 ggagagtacg cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca
    18421 cttcgcattt aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt
    18481 atgtttaata cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag
    18541 cgacggggca cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca
    18601 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca
    18661 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
    18721 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
    18781 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
    18841 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
    18901 ataactccac aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac
    18961 taaaggttga ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct
    19021 accacactat gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc
    19081 cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac
    19141 tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg
    19201 ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg
    19261 ccgatcgcaa ggtgaatcaa ccacgtgcct taattatggc accgacgcgt gaacttgccg
    19321 tgcagatcca tgccgacgca gaaccgctgg cggaagctac cggcctgaag ctgggtctgg
    19381 cttacggtgg tgatggctac gacaaacagc tgaaagtgct ggaaagcggc gttgacattc
    19441 tgattggcac tacggggcgt ttaattgact acgccaagca gaaccacatt aacctcggtg
    19501 ccattcaggt ggtggtactg gacgaagccg atcgcatgta cgatctgggc tttattaaag
    19561 atatccgctg gctgttccgc cgtatgccgc ctgcaaacca gcgcctcaac atgctgttct
    19621 ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat
    19681 atattgaagt ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc
    19741 cttctaacga agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca
    19801 gagcgattat tttcgccaat accaaatacc gttgtgaaga gatctggggc cacctggcgg
    19861 cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta
    19921 ttcttgatga atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc
    19981 gtggtttgca tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg
    20041 aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca
    20101 gcctggcgtg tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact
    20161 caattccggt aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc
    20221 gcctcacgcg cccacgtaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc
    20281 gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa
    20341 gagaatgaat cccgatgagc atactgtagt aagtgattca ggtgaaagaa tgcagccaac
    20401 acagagacag attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt
    20461 gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag
    20521 acgctgacgc gaataaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc
    20581 ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg
    20641 caagatatcc ctccctcgca aattcgcgtt gtcgctacgg cgacattacg tcttgccgtc
    20701 aatgcgggtg attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc
    20761 agcggtgaag aggaagcacg tctcatttat cagggcgttg ctcacacaac tggcggtgcc
    20821 gatcagcgcc tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt
    20881 gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat
    20941 tttgccgatc gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa
    21001 gtgttacgtc cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct
    21061 tccggcaccg tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt
    21121 accctggaaa agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa
    21181 ctggagattg acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc
    21241 ctgatcgcca tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg
    21301 cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt
    21361 acgctgcgta atattcagcg ccgctttatg atcgatattg atcaggcgca gcgcgtagcc
    21421 aaagttgcgg ctaacttctt cgatcaggtg gaaaaggaat ggcatcttga agcaataagc
    21481 cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgactttaaa
    21541 caagcgccgc aacacgctgc ttatctggtg cgtaatctgg atcttcccgg ttttaccccc
    21601 gcacagaaaa aattgctggc gacgctactg ctcaaccaga ctaatccggt cgatctctca
    21661 tcgctgcatc agcaaaatgc cgtacctccg cgcgtcgcag aacaactctg ccgtttactc
    21721 cgcctggcca tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta
    21781 caggctaacc atgaactgtt gaccttgacg cttccgcaag gttggctaac tcaacatccg
    21841 ctgggtaaag agattattgc tcaggaaagc cagtggcaga gctatgtcca ctggccgctg
    21901 gaagtgcatt aatgagtaag tgccggatgc gatgctgacg catcttttcc ggccttgatt
    21961 atttccctcg ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct
    22021 gcatccgttc ttcggcgctg accactttgc gctcctgttc ccaaatcaga tcatcctgcg
    22081 gcagctccag caaaaagcgg ctcggctccg ggcgcaccag ctcgccgtac tgacggcgtt
    22141 ctttacacag cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc
    22201 gccgctcctc atcgatatta tcttcatcga tgctgctctg gtgcggcaaa aacccttctt
    22261 ccataccgac catgtagaca taaggaaact ccagcccttt tgacgcgtgc agagtcatca
    22321 gttgcacctg atccagctct tcttcactct caccacgctc catcatgtcg cgcaaagtaa
    22381 agcgcgtcac cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct
    22441 ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg catttcggcg gctttcgggc
    22501 tgggcgatgt ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg
    22561 cggcaatcgg ctcccgctcc gccagacgct ggatttctgc caaccagtga gtaaagcggg
    22621 tcaatgcttc ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg
    22681 taaacatgct tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc
    22741 caatctctct cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag
    22801 tcagcacgcg cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac
    22861 caccagaaat tttgtacggg atgcggtttt gcatcaggaa tttttcaaac actcgcgact
    22921 gatggttacc gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat
    22981 gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa
    23041 ctttaagctc tgtgccgtaa cccagttcgg agaacagacg cttctcaaag acgtgcgggt
    23101 tattggcgat caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct
    23161 caagcttaat caccttcagc gccggaaaat cctgactcag cagcaccagg ttttgtggac
    23221 gtgcgccgcg ccaggagtag atcgactggt cgtcatcgcc caccacggta aagcgcgcgc
    23281 ggctgcccac cagcagtttc accagttcat actggctggt gttggtgtcc tgatactcat
    23341 ccaccagcag atagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca
    23401 gcagcaacgt cggcaataaa atcagatcat cgaagtcgag aacattgcac gctttcaggt
    23461 gtgcatcata cagcccataa caatgggcaa aaatacgatc ccgctcgccc tttgcctctg
    23521 ccgccgcctg cgccggagtt ttgagatcgt tcttccagtt ggagatggtc gaaatcagct
    23581 gttgcaggag aactttgtca tcttcaatca gcccctcggt caactcttta agcaaagcaa
    23641 gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat cccaagcgcc gcatactcgc
    23701 gtttgatgat atccaacccc agcgtgtgaa aagtggagat catcaacccg cgcgcctctt
    23761 tgcgacccag cgtctgcccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg
    23821 tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat
    23881 tggtgatcac acgagtttta ccggaacccg cgcccgccag caccaggcag gggccggtaa
    23941 cgaattcgac agcttgttgt tggccggggt ttagacgcat aggtgtattg ctcaatcttc
    24001 gaacgggggg aggattgtag cagaatcgca tttgaatgtg accgcgtgta agccgctaac
    24061 cggttggctg cggatgttta aatcagagag agaagaccga aatcggagtt catcgtgaat
    24121 ccattagact tagaaaatat cgggtcgtaa cgttgtgtta ttactttttc agagtttact
    24181 aaggagaggt ttactcgtgg cgattaattt cagccctaag gttggtgaaa tacaggaatg
    24241 caactttggt aactatcctg ttatccagaa tggacagttc agcaccacgt attacgatgg
    24301 ccgcattcct cctgaaatga taaaaaacag gttagttgtc gtgctcaacg gaaaaattaa
    24361 tggcaacgca tgtatcgtcg ttccattatc aaccactcgc gatcatgaca agttaaatcg
    24421 tggaatgcat gttgaaatag ccagtaatgt tatcaatggc ctgcaattct ttgatcagca
    24481 gattcgttgg gccaaagccg acctggtaca acaagtcagt cgaaaccgat taaatagagc
    24541 aaggacttac agagggtatc ttaatcaatg tttgccacat gagttggttg cagacataca
    24601 gagggcggtc atcaagtcaa tcaatgccat atcgctcata aattgacaat aaaataacca
    24661 tccccgataa tgactttgta gcccatagag gccctaagtt ttctcccctt gagggacctg
    24721 cgaagcccgg attttccggg cttttgccat atatgtacca gcccctccct gactgacaat
    24781 tacgttagca tctgcggatc atgctaaaat cgccgcctga caattattca ctcaaggcac
    24841 gatcatggca aaaacagcag cagcactgca tatccttgta aaagaagaga aactggctct
    24901 ggatcttctc gagcagatta agaacggtgc cgatttcggc aagctggcga agaaacactc
    24961 tatttgcccg tcaggcaaac gcggcggtga tttaggtgaa ttccgccagg gtcagatggt
    25021 tccggcgttc gataaagtgg ttttctcttg tccggtactg gagccgaccg gcccgctgca
    25081 cacccagttc ggatatcaca tcattaaggt gctgtaccgc aactaatagc aaggccttct
    25141 ccaggagaag gccttgagtg ttttctccct ctccctgtgg gagagggtcg gggtgagggc
    25201 atcagcgcgc acttaacccg caacagcaat acgtttcata tctgtcatat agccgcgcag
    25261 tttcttacct acctgctcaa tcgcatggct gcgaatcgct tcgttcacat cacgcagttg
    25321 cgcgttatct accgcgcctt ccggaatagc tttacccaag tcgcccggtt gcagctctgc
    25381 cataaacggt ttcagcaacg gcacacaagc gtaagagaac agatagttac cgtactcagc
    25441 ggtatcagag ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat
    25501 cagcggcagc tcgtgcagtg attcataata tgcagactct tcaatgatgc cggaatcgac
    25561 catggtttcg aacgccagtt caacgcccgc ttttaccatc gcgatcatca gtacgccttt
    25621 atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt caaacgcggt
    25681 tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat
    25741 cataccggaa gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggtgc
    25801 catgatctct ttcagctgtt ctgacagcgc ataagcacgc agtttcgccg ggttagagag
    25861 acggtccatc atcagggtga tgccgccctg cttcagcgct tcggtgatgg tttcccaacc
    25921 gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca gcttgtcgaa
    25981 gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac ccatcaggtc
    26041 agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac caccggttgc
    26101 tgccgcccat gctttagcga tcgccatacc ttcgcctttc ggatcgtttt ccgggtgaac
    26161 ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt cggtgccagg
    26221 gcatttcggc gcaaccatga ctacggtgat atctttacgg atctgctcgc ccacttcgac
    26281 gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt
    26341 gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgcgg
    26401 gatcagttct tcgtaagtac caactttaaa accgttttcg gtcgctttac gccaggacgc
    26461 gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatccagac cagaatcacg
    26521 catgttcagg ccctggttca gaccctgtgc gccacagccg acgatgacta cttttttacc
    26581 ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac atttgcccag
    26641 ctgtgccagc tgctgacgca gattcagtgt attgaagtag ttagccatgg tgattcctcg
    26701 tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac tatatgacag
    26761 gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaattttt gcaacgtcaa
    26821 catcgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    26881 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccatccacg ctctcacggc
    26941 agattcagcg cctggaagaa gatcttggtc agccgctatt tgtgcgcgat aaccgcacgg
    27001 taacgctgac ggaagcgggc gaagagctgc gcgttttcgc ccagcaaacg ctgttgcagt
    27061 atcagcaatt gcgccacacc atcgatcagc aagggccgtc gctctctggc gaattacata
    27121 tcttctgctc ggtgaccgct gcctacagcc atctgccgcc gattctggat cgcttccgcg
    27181 cggaacaccc gtcggtggag atcaaactca ccactggtga tgcggcagat gcaatggaaa
    27241 aagtggtcac gggagaagcg gatctggcga ttgccgggaa accggagacg ctacccggcg
    27301 ctgtggcgtt ttcgatgctg gagaatctgg cagtagtgct gattgcgccc gcgctgccct
    27361 gcccggtgcg taaccaggtt tcggcagaga agccggactg gtctacagtg ccgtttatca
    27421 tggccgatca gggaccggtg cgtcgccgca ttgaactgtg gtttcgacgc aataaaatca
    27481 gcaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    27541 tcggctgtgg tgtggcgctg ttgccggaag tagtgctgga aaacagcccc gaaccggtgc
    27601 gtaaccgcgt gatgatttta gagcgcagcg atgaaaaaac gccgtttgaa ctgggcgtgt
    27661 gtgcacaaaa aaagcggctg catgagccgc tgattgaggc gttctggagg attttgccga
    27721 accacaaatg acgttgtcgc gcgggtaggc ctgataagcg aagcgctatc aggcattttt
    27781 ccctaacccg ccaaaaagaa cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag
    27841 cccagctcat tcagccgggt ttcgaaatcc ggttcatggt cgccaagttc gaacgccgcc
    27901 agtacgcgcc cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc
    27961 gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg
    28021 tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac
    28081 ttcgccattt cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc
    28141 aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg
    28201 tttttggcat cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg
    28261 cagaatttga ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc
    28321 tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca
    28381 ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc
    28441 attcccgcca gcgccagcgc gccagagggt tccgccaccg cgcgcacatc ttcgaataaa
    28501 tccttcatcg ccgcacagat cgcatcacta tcgacggtga tgatgtcgtc gagatactcc
    28561 tggcataaac ggaaggtttc gtcaccgatg cgttttaccg ctacgccttc agcaaatagc
    28621 cctacgcgcg gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag
    28681 tcttccgctt ctacggcgat cactttgatt tgtggcatca gttgtttgat cagcaccgca
    28741 acgcccgccg ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc
    28801 tgctggagca gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac
    28861 ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga tcgctttagc tttcgcttcg
    28921 tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc cacgtaccgc atcgactttg
    28981 atgtcggcgg tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa
    29041 aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt
    29101 tcttccgtca ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc
    29161 tggcgatctt cgcgcttcac cagaatgacg ttatcaagac gcgacgacag tttttccatt
    29221 ttttgtagcg gcgtaacctg cgccgcctcg taaaccggcg cgcgcagcac tgctcttaaa
    29281 tattcggcac cttccggagc accggacagg ggttgcgagt cagccattat taacccccca
    29341 gtttcgattt atcgcgcacc gcacctttgt cggcgctggt tgccaggctg gcgtaggcgc
    29401 gcagggcaaa ggaaacctga cgttcacggt ttttcggcgt ccaggctttg tcacctcgag
    29461 cgtcctgcgc ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac
    29521 ggttcgggat gtctatagcg atcaggtcac catcttcaat caggccaatg ctgccgccgc
    29581 ttgccgcttc cggtgagacg tggccgatgg aaagaccaga ggtgccgcca gagaaacgac
    29641 cgtcggtgat cagcgcacag gctttaccga gacccattga tttcaggaag ctggttgggt
    29701 agagcatttc ctgcatgcca gggccgcctt tcgggccttc atagcggatc accaccacat
    29761 cgcccgcgac aactttgcca ccgaggatcg cttctaccgc gtcgtcctgg ctttcgtaca
    29821 ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac gcccgcggtt ttaacgatgc
    29881 agccgttttc tgcgaaatta ccgtagagca ccgccaggcc gccgtctttg ctgtaggcgt
    29941 gttccagcga gcggatacag ccattggcgc gatcgtcatc gagagaatcc caacggcaat
    30001 cctgcgagaa tgcctgtgtg gtacgaatgc ctgcaggacc tgcgcggaac atatttttta
    30061 ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag cgtttgcggc aacgtcaggc
    30121 caagtacgtt tttcacatca cggttcagta accccgcgcg atccagttcg ccgagaatac
    30181 cgataacacc accagcacgg tgaacatctt ccatatggta tttctgggtg ctcggcgcaa
    30241 ctttacacag ctgtggaacc ttgcgggaaa gcttatcgat atcactcatg gtgaagtcga
    30301 tttccgcttc ctgcgccgcc gccagcaggt gaagtacggt gttagtcgat ccacccatcg
    30361 cgatatccag cgtcatggcg ttttcaaacg ccgctttgct agcaatattg cgcggcagtg
    30421 cactttcgtc gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccggcat
    30481 tgaggaacaa ctgcttacgg tcggcgtggg ttgccagcag cgagccgttg cccggctgcg
    30541 acagacccag cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg
    30601 agccgcaggt cggacacgcg gaacgttcaa cctgatcgct ctgggagtca gaaactttcg
    30661 ggtctgcgcc ctggatcatc gcatcaacca gatcgagttt gattatctga tcggacagtt
    30721 tggttttccc ggcctccatc gggccgccgg aaacaaagat caccggaata ttcaggcgca
    30781 gggaagccat cagcatcccc ggggtgattt tgtcgcagtt agagatgcag accatggcgt
    30841 cggcgcagtg ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggca
    30901 gtgaataaag catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga
    30961 actctttggc aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc
    31021 gcagatggac gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct
    31081 tgccaaaatc ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat
    31141 tacgaccatg agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt
    31201 ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga
    31261 tctaaccagc cccatttatc ttcggtttcg ccagtgaaga ggccgaagaa ggcttgctga
    31321 atgcgtttgg taaccgggcc acaacggcct tcgccaacct gaataccatc tacactgcgc
    31381 actggcgtga tttctgccgc agtaccggac ataaacactt catccgccag gtacagggat
    31441 tcgcgcgaca gaacctgctc acgcacttca attcccagct ctttcgccag tttgatgatg
    31501 gcatcacggg taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca
    31561 tctttcactt caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc
    31621 gcgatacctt cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagaga
    31681 tagttaccac cggcttttgc cgcggttggg atggtgtttg gtgctgcgcg gttccaggag
    31741 gaaaccatcg catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg
    31801 aaagcggcga taatcacatc ggttgagtat cccgctggcg ggtttacacc catgccaaca
    31861 tcaccaacga agatcagcgg acggatatag gcgctggtga gattgttttt gcggatcacg
    31921 tcacgacaag cttccatcag ctcatcaatg ctctgcgaaa ccgggaagcg atagattttg
    31981 gcggagtcat gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgc
    32041 gagtcgtagc aacggatgcc ttcaaaaacc gaagtgccat agtgcagcgc gtgcgacatc
    32101 acatgcacct tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct
    32161 ttcttcgtgg tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt
    32221 tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag
    32281 taagtcgacc gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct
    32341 ggcggcagcc atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac
    32401 acgttctaag gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat
    32461 aatttctcca acatttctga attactggcg ccaggcggca ccagcggcca gacgttctca
    32521 agttcgtcga ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt
    32581 gccgcttcaa cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct
    32641 aacatgagga aatcggggtt atcagtaagg gtggtttcgc tgtatcgttc ctgaaaaaac
    32701 agttgctgcc attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac
    32761 ggtaactgct tgcgttttac ggtgcccagc tcttgcacat tcatcatgaa agagccgtca
    32821 ccggagatac agacgacagt atcgttcggt cgtgcgactt gtgcgccaac cgccgctggt
    32881 aaaccgaaac ccatggtgcc taagccgctg gaggtaatga aattttccgg gcgagtgtgt
    32941 gcgatgtgct gcgcggccca catctggtgc tgccccacat ctgtggtcac gacgcaatcc
    33001 gcaggtttac gatccgacag ttgttttaac aacaacggcg cgtagatagc gtcaccggga
    33061 tggtcgtaac gccaggtatg ttcatcacgc agctgcgcgc agtattgctg ccagtcattg
    33121 atatttaacg gctgctgtaa tgctggtaac agagcattta aatcaccttg taatgccaca
    33181 tgtgcctgac gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg
    33241 tgtggtgcga aggtgttcag tttgccggtc acccggtcat caaaacgtgc acccacggcg
    33301 atcagcaagt cgcactcctg caccgcgaag ttcgccgctt tggtgccatg cattcccagc
    33361 atgcccaggt agtacggata atctgcttca actgcgccca gccctttcag cgtgcaggtg
    33421 gcaggcattt ttgtggtagc gagaaattct cgtaaagcag gaactgcctg cgccataccc
    33481 acgccaccac caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc
    33541 tcaacttcgg catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccagg
    33601 tcaccgctgg ctagctggat atcttttggg atatcgacca gaaccggacc aggacgacct
    33661 gagctggcaa cgtcgaatgc ttcagccata atgcgcggca actcttccag cgactgcacc
    33721 agaaagctgt gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg
    33781 tccgtgccga taaacggtgc ggacacttga ccggtgatgg caacaacagg gatagaatct
    33841 aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata
    33901 catacgccag ttttgccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc
    33961 tcatgtcggc acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata
    34021 attgcgccac ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc
    34081 acccactgtg cgccattcat agttagttcc ccgtcctgaa tcttgagaaa cagaattttg
    34141 tgctgttatt cattgtctgc tcctcggtta tatttttaag gtcaaaaaaa acccccggac
    34201 ctttcggtgc gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag
    34261 tgcagccccg cacggtggga taataatcac caccacgcta atcatgacca ggctaatcac
    34321 tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag
    34381 ttaccataga ttttgcgaat agcacaagat attttttggc cgatttttat gatgcgttta
    34441 ataatttcaa tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat
    34501 ttcagtatga atgttgttcc cttcctcgta gttcatcctt tgtccgctgc atttttgcga
    34561 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
    34621 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tagtgactca
    34681 aggagggctt atgtcactgt caattgttca tacccgcgca gccctgggag taaatgcgcc
    34741 cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga tggtgggctt
    34801 accagaaaca actgtaaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
    34861 atatgaatat ccggcgaaaa aaatcaccat caacctggct ccagccgatc tgccaaaaga
    34921 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
    34981 agccaataag ttagatgaat atgaattagt cggagaactg gcgcttacag gcgctctgcg
    35041 tggcgttccc ggcgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
    35101 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc
    35161 cgatcatctg caggctgtct gtgcgtttct ggaaggtaag cacgctctcg aacgcccgaa
    35221 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
    35281 gcaaggaaag cgaggactgg aaattaccgc cgctggcggg cacaaccttt tactgattgg
    35341 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt tgccagattt
    35401 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
    35461 acaaaaacaa tggcggcagc gcccgttccg ctcaccgcat cacagtgcgt cgttaactgc
    35521 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
    35581 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
    35641 gattgaatcc gggcagatcc atctttcacg cacacgagcc aaaataacct atccagcccg
    35701 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
    35761 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
    35821 ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    35881 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    35941 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    36001 caaacttgag agcgaagatg cgatgtggtt ggaaggaaca ctgatccatc tggggttatc
    36061 gattcgtgcc tggcagcggt tattgaaagt tgcacgaacc attgctgata ttgatcagtc
    36121 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    36181 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    36241 catcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    36301 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    36361 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    36421 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    36481 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    36541 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    36601 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    36661 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    36721 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    36781 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    36841 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    36901 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    36961 agttttctat cggtgccagc gcctcattgt gggaatgtat gcttaatcag tggctgggac
    37021 gtttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    37081 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    37141 cgcccaaaat ggacgaattt agtagtcagt tgctgggata tttcacttta gcgctttata
    37201 ccagtgcccc ttcaaaacta aagggagatc ttaattatct gcgacttgag tgggggccag
    37261 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agtgcccatt ctgacaacca
    37321 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    37381 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    37441 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tacgttgatt cgcgatcttt
    37501 tgaaaattaa cgtgctggat gaagtgtatt aatatgaatg gctggcaagg atgccggtag
    37561 aaggatttac ttcggagagg gttatttcag ataaaaaaaa tccttagctt tcgctaagga
    37621 tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc
    37681 tctaccaatt gaactacgcc cctaattagg gtggcggaac ggacgggact cgaacccgcg
    37741 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
    37801 tacaaccacc ggttttatga ccggctt
//
LOCUS       DAZFDP010000027        37431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFDP010000027 DAZFDP010000000
VERSION     DAZFDP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..684)
                     /locus_tag="ACHJ1G_001530"
     CDS             complement(<1..684)
                     /locus_tag="ACHJ1G_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4763406.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(695..1696)
                     /gene="ltaE"
                     /locus_tag="ACHJ1G_001531"
     CDS             complement(695..1696)
                     /gene="ltaE"
                     /locus_tag="ACHJ1G_001531"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4763407.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1733..3451)
                     /gene="poxB"
                     /locus_tag="ACHJ1G_001532"
     CDS             complement(1733..3451)
                     /gene="poxB"
                     /locus_tag="ACHJ1G_001532"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4763408.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3584..4552)
                     /gene="hcr"
                     /locus_tag="ACHJ1G_001533"
     CDS             complement(3584..4552)
                     /gene="hcr"
                     /locus_tag="ACHJ1G_001533"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4763409.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4564..6216)
                     /gene="hcp"
                     /locus_tag="ACHJ1G_001534"
                     /gene_synonym="priS"
     CDS             complement(4564..6216)
                     /gene="hcp"
                     /locus_tag="ACHJ1G_001534"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4763410.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6360..7259)
                     /gene="lysO"
                     /locus_tag="ACHJ1G_001535"
     CDS             complement(6360..7259)
                     /gene="lysO"
                     /locus_tag="ACHJ1G_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4763411.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7754..8449)
                     /gene="aqpZ"
                     /locus_tag="ACHJ1G_001536"
     CDS             complement(7754..8449)
                     /gene="aqpZ"
                     /locus_tag="ACHJ1G_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4763412.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8875..10533
                     /gene="ybjD"
                     /locus_tag="ACHJ1G_001537"
     CDS             8875..10533
                     /gene="ybjD"
                     /locus_tag="ACHJ1G_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4763413.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10530..11486)
                     /gene="ybjX"
                     /locus_tag="ACHJ1G_001538"
     CDS             complement(10530..11486)
                     /gene="ybjX"
                     /locus_tag="ACHJ1G_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4763414.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11637..12752
                     /gene="macA"
                     /locus_tag="ACHJ1G_001539"
     CDS             11637..12752
                     /gene="macA"
                     /locus_tag="ACHJ1G_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4763415.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12749..14695
                     /gene="macB"
                     /locus_tag="ACHJ1G_001540"
     CDS             12749..14695
                     /gene="macB"
                     /locus_tag="ACHJ1G_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4763416.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14768..14992)
                     /gene="cspD"
                     /locus_tag="ACHJ1G_001541"
     CDS             complement(14768..14992)
                     /gene="cspD"
                     /locus_tag="ACHJ1G_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4763417.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15142..15216
                     /gene="yljB"
                     /locus_tag="ACHJ1G_001542"
     CDS             15142..15216
                     /gene="yljB"
                     /locus_tag="ACHJ1G_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4763418.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15315..15635
                     /gene="clpS"
                     /locus_tag="ACHJ1G_001543"
     CDS             15315..15635
                     /gene="clpS"
                     /locus_tag="ACHJ1G_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4763419.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15666..17942
                     /gene="clpA"
                     /locus_tag="ACHJ1G_001544"
     CDS             15666..17942
                     /gene="clpA"
                     /locus_tag="ACHJ1G_001544"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4763420.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18285..18372)
                     /locus_tag="ACHJ1G_001545"
     tRNA            complement(18285..18372)
                     /locus_tag="ACHJ1G_001545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18336..18338),aa:Ser,seq:gga)
     gene            complement(18626..18844)
                     /gene="infA"
                     /locus_tag="ACHJ1G_001546"
     CDS             complement(18626..18844)
                     /gene="infA"
                     /locus_tag="ACHJ1G_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4763421.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19129..19833)
                     /gene="aat"
                     /locus_tag="ACHJ1G_001547"
     CDS             complement(19129..19833)
                     /gene="aat"
                     /locus_tag="ACHJ1G_001547"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4763422.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19875..21596)
                     /gene="cydC"
                     /locus_tag="ACHJ1G_001548"
     CDS             complement(19875..21596)
                     /gene="cydC"
                     /locus_tag="ACHJ1G_001548"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4763423.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21597..23363)
                     /gene="cydD"
                     /locus_tag="ACHJ1G_001549"
     CDS             complement(21597..23363)
                     /gene="cydD"
                     /locus_tag="ACHJ1G_001549"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4763424.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23486..24451)
                     /gene="trxB"
                     /locus_tag="ACHJ1G_001550"
     CDS             complement(23486..24451)
                     /gene="trxB"
                     /locus_tag="ACHJ1G_001550"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4763425.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            24995..25489
                     /gene="lrp"
                     /locus_tag="ACHJ1G_001551"
     CDS             24995..25489
                     /gene="lrp"
                     /locus_tag="ACHJ1G_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4763426.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25624..29730
                     /gene="ftsK"
                     /locus_tag="ACHJ1G_001552"
     CDS             25624..29730
                     /gene="ftsK"
                     /locus_tag="ACHJ1G_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4763427.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29885..30496
                     /gene="lolA"
                     /locus_tag="ACHJ1G_001553"
     CDS             29885..30496
                     /gene="lolA"
                     /locus_tag="ACHJ1G_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4763428.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30507..31850
                     /gene="rarA"
                     /locus_tag="ACHJ1G_001554"
                     /gene_synonym="mgsA"
     CDS             30507..31850
                     /gene="rarA"
                     /locus_tag="ACHJ1G_001554"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4763429.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31941..33233
                     /gene="serS"
                     /locus_tag="ACHJ1G_001555"
     CDS             31941..33233
                     /gene="serS"
                     /locus_tag="ACHJ1G_001555"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4763430.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(33534..33674)
                     /locus_tag="ACHJ1G_001556"
     CDS             complement(33534..33674)
                     /locus_tag="ACHJ1G_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4763431.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(33866..34126)
                     /locus_tag="ACHJ1G_001557"
     CDS             complement(33866..34126)
                     /locus_tag="ACHJ1G_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4763432.1"
                     /translation="MFRCPLCGASARIRTSRSENDSNTVRKKYYQCNNLECGISFSTL
                     EAFHKFTSKHAPGVHSSEGIPWHELPASHRGNNQMSLPLSQN"
     gene            complement(34169..35278)
                     /locus_tag="ACHJ1G_001558"
     CDS             complement(34169..35278)
                     /locus_tag="ACHJ1G_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO4763433.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            35436..36620
                     /locus_tag="ACHJ1G_001559"
     CDS             35436..36620
                     /locus_tag="ACHJ1G_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4763434.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCDTMADAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            36620..37132
                     /locus_tag="ACHJ1G_001560"
     CDS             36620..37132
                     /locus_tag="ACHJ1G_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4763435.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            37187..>37431
                     /locus_tag="ACHJ1G_001561"
     CDS             37187..>37431
                     /locus_tag="ACHJ1G_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4763436.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRL"
BASE COUNT         9216 a         9504 c        10036 g         8675 t
ORIGIN      
        1 cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc
       61 ggcggcttcg aagatgcggt gttcgctggc cggatgatct aacaacgcca ccagatagtg
      121 cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa
      181 cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac
      241 gataattccg gcccgaagtt cggtcacagg tacattcgct tcacgaagaa tgtccgccgt
      301 agcctgacga gcacgcagat gatccgactg ctcatgtggc ggggcctgca acgaactgag
      361 aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga gagccacctg
      421 gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa aatagacggt
      481 atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga ctttatggca
      541 actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgccaggat
      601 ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc
      661 gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat gcgcgccagt
      721 gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa
      781 tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac gcggcagcat
      841 tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg
      901 cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga
      961 cgttattttt cagggcatac atcccggcgg cagccagaat cccggactga cgcatcccgc
     1021 caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta ccgacgagta
     1081 atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt
     1141 gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg cgcgcaccgt
     1201 caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc aggtattcac
     1261 gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat
     1321 ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag
     1381 ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca
     1441 gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag tgactgagca
     1501 gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa
     1561 gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc
     1621 caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta acggtatcac
     1681 tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta
     1741 gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata attgcgcgca
     1801 acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaact
     1861 cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag gcgcgttgca
     1921 gggcttcatc aacttcagac gctttttcta cacggatacc cgtaatgccg cacgcttcgg
     1981 caatgcgggc aaagtttgtg tcgtgtagtt cggtgccgtc agtcaaatag ccaccagctt
     2041 tcatctccat cgccacaaag cccagcacgc tgttgttaaa gacgacaatt ttcactggca
     2101 gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc
     2161 acatggcgac cacctgacgt tctggctctg tcgcctgcgc acccagcgcc tgcggcatgg
     2221 cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttta
     2281 gataacgtgc cgcccacacc gttggcgtac caacgtcaca ggtgaaaata gcgtcatcgg
     2341 cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt
     2401 tagctaaatc gtccagccct ttgcgggcgt cgcggtaatc ttccagcgct ttatccagaa
     2461 acttgcgatc ggctttttct tccaccaatg gaagcaatgc acgcagagtc gacttgatat
     2521 cgccgaccag tgccatatcc accttgctgt gagcgccgat gctggctggg ttgatatcaa
     2581 tctgaatgat tttggcatcg gtcgggtaga aggcgcggta gggaaattgc gtgccgagta
     2641 gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaagccg attaacccgg
     2701 tcattccaac atcatacgga ttatcgtatt cgacatgttc tttaccgcgc agggcatgaa
     2761 caataggcgc tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc
     2821 cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc agtttgcgta
     2881 actcttcttc ttccggcgtc acgactggtt gtggcgcatg ataccagtgc gtggttgccc
     2941 cttctggcgc aggttttaac gccacgtcgc ctggtaacac gacaaccgaa acgccacggt
     3001 taagcaccgc tttgcgcatg gcaatcgcca gtacttgtgg gatctgctcc gggctggaaa
     3061 ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat
     3121 agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc ggaacgtgat
     3181 tgcggtggca atcgaacagg ccgttgatta agtgcaggtt gccggggccg cacgatccgg
     3241 cgcagaccgc cagttctccg ctaagttgtg cttcagcgcc agcggcaaag gccgccactt
     3301 cttcgtggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg tcactaagac
     3361 cgttcagaga gtcgcctgtg actccccaga tgcgtttcac ccctgccgat tcgagtgttt
     3421 tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg
     3481 taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg
     3541 gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat
     3601 ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca
     3661 gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta cagcagccgc
     3721 aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc gcctccagta
     3781 gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg aatttcagac
     3841 cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc
     3901 cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca
     3961 cggtacgtga agctaagtca ggtacacctg ccagcagttc gcgagtgaga cgaccagcga
     4021 taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta cgccactcat
     4081 cggcgaaaat aacatcctgt ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg
     4141 gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgccg
     4201 ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtccg
     4261 aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag ccgacaccgt
     4321 catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg
     4381 tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat tgcccggcgc
     4441 gatatgggta gtaatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgcg
     4501 taatgtgatg aacctgcatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac
     4561 tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc
     4621 aaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc
     4681 ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacga tcgctttctg
     4741 ttcaaaccag gagagcacca gcgacagcgg cagatcgttg acaccgcagc ccagtttctc
     4801 tgccagagtg acagccagaa taatcgctga gtaagcatcg ttacattgac ctgcatctac
     4861 cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca
     4921 ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg
     4981 gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg
     5041 gctcaccaga tcaatcagcg tatcagcagc gccaagcagc gtctggcgac caaaacccac
     5101 ggtgataagg tgcggaattt cgctgtacgg gaagcctgcc atctgttgcg cctgggtgat
     5161 aaccgcagag aaatcatcac cgtccagatg acgcacgcca ggccagccaa caatgctgcg
     5221 ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat
     5281 cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc cactgccgta
     5341 gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc
     5401 gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc
     5461 tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga cgttgacctg
     5521 ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt
     5581 gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca
     5641 ggtccccagc cacgccatga ttttatgata ctgggcataa atatcgttgt cgtattgacc
     5701 gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag
     5761 acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc
     5821 agcctgacgt tgcagctcgc cgagatcgtc gctcaccagt tgcaggtcag ccatcgggtt
     5881 atcgacgcgc gcgttggcat ctacagccag gcattgcgct ttcagcgcct cgcgcagggc
     5941 aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagagt
     6001 tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg
     6061 cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc
     6121 agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg
     6181 agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt
     6241 taatatacat gtttaaggtt aagacgctta acgcggggat aaaagggatt tttcatgcaa
     6301 ctttaaggga gattgattta gcgcaatttt ggcggcagga atctaccgcc agagaggtat
     6361 tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca
     6421 attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc
     6481 attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat cagcccaggg
     6541 atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc gctcccgatt
     6601 accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc gaaaccggag
     6661 gccattgcca gcgaggtatt gatggggaga tcaagaataa aggcgttaat taaaccacca
     6721 attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc
     6781 tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt
     6841 aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg
     6901 gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat cgcaatacgc
     6961 gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc cagccacatc
     7021 agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc
     7081 gccaacaggt tactggcgag gttatcgagg aacgccagac ttatacccat aaaaaagaga
     7141 ataaggtaaa ccatccagct taatagctga ttaataactt ttaacgcagc ttgttggcga
     7201 agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc agaaaacatg
     7261 aaaacccagt ccttgcaaag atgaagtcga aatgcgcgat gacacactac tgaaagcgga
     7321 aggacgagta aagttgcaat taaaaggaaa tgttatgcat aaggagcagt agagtattcg
     7381 ttttcattta aagatattct tgcgctttaa ttacaaactg caccgatgtt ggtggcgtca
     7441 aaatcgccga ggcgttccct gaaggccggg gcagcccaca tggatgtggg ctgagggcgc
     7501 gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa gtcgagggca
     7561 ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc atggggcggc ggcgattggc
     7621 cgccccctgc gcgctccttg cgccagtggc aatatgtggc ttagctcatg aaaggagcgc
     7681 aacaagatga tgaatcaaca tataacaaca tcttaaaaaa aggcctgaca ttacgccagg
     7741 ccttctgcgt taattaatca cgcttttcca gcagggtccg gtaaatcaga ccaccgataa
     7801 tgccgccgac aattggcacc acccagaaga accacagttg ttctaatgcc cagccgccct
     7861 ggaagatagc aaccgcggtg ctgcgcgccg ggttaacaga agtgttagtc accggaatac
     7921 taattaagtg aatcagggtt aaggccagac caatagcgat cggcgcaaaa cctgccggcg
     7981 cgaatttgtc ggttgcgccg tggatcacca acaggaaacc tgcactcaat accagttcaa
     8041 ctaccagcgc ggaaagcatg gaataaccgc ctggtgaatg ctcgccataa ccgttagaag
     8101 caaaaccgct ggctgccgcg tcaaaacccg ttttaccact ggcaattaaa tacagcagcg
     8161 ccgctgcaac aataccgccg acaacctggg caattacgta gccaacgact tcttttgccg
     8221 gaaaacgtcc gccagcccat aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa
     8281 tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc gaacgccaac gccacgccgg
     8341 caaaaccaat gcctaattcc gggaagcctg cggccagtac agcactacca cagccaccaa
     8401 aaacaagcca gaaagtacca aaacattcag ctgctaattt tctgaacata tccacctcaa
     8461 ttaaaaattg accctgtgaa aaatatggtc gttttatagg gtcgtcgtaa aaaagtgacg
     8521 acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt cattcaccag ataaataaat
     8581 ccagtaaatt tgatttaggg caacagcggg ttgccccata tagtcatttg tctgattgac
     8641 agtgtagtgc aagcaaaaga tttaatcctt taggcgtaat aaaaaataat ttatcatgct
     8701 aattatttga ttttgttgtt tttgcagact tatcagcaag agggagtata acgcgattat
     8761 tcgctcattt ttcagacatt tgccatgctt aaatgtgatg tcatcacgta ttagcaaggc
     8821 ctttcccgtt atactgccag cgtaaaggat aagtcacata tttctggagg ggatatgatt
     8881 cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc gtttgtcgtt gatgctcgaa
     8941 caaaacaacg tcctgattgg ggagaacgcg tggggtaaat ccagcttgct ggacgcctta
     9001 accctgctgc tatcgccaga atcagatctc taccattttg agcgcgacga tttctggttc
     9061 ccgccgggag atatcaacgg gcgagaacat catctgcata ttattttgac cttccgcgaa
     9121 tcgctgccag gccgacatcg ggttcgccgt tatcggccgc tggaagcgtg ctggacgcca
     9181 tgcaccgatg gctatcaccg tattttttat cgtctggaag gggagagtgc ggaagacggc
     9241 agcgtgatga cactgcgcag ttttctcgat aaagacggac atccgattga tgtcgaggat
     9301 attaacgatc aggcacgcca tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc
     9361 cgttttatgc gccgtattcg taacggcacg gtgccaaatg ttcccaatgt ggaagtcacc
     9421 gcgcgccagc tcgatttcct cgcccgtgag ttgtcctcac atccgcaaaa tctctctgat
     9481 ggacagattc gtcagggact ttccgcaatg gtgcagttgc ttgagcatta tttctctgag
     9541 cagggggctg gacaggcgcg atatcgttta atgcggcggc gggccagcaa tgagcaacga
     9601 agctggcgct atctggacat catcaaccgg atgattgacc gacctggagg gcgctcgtat
     9661 cgggttattt tgctcggcct gtttgctact ttattgcagg caaaaggcac attgagactg
     9721 gataaagacg cgcgtccgtt attgctaatt gaagatccag aaacccgttt acaccccatt
     9781 atgctttcag ttgcctggca tctgttgaat cttctgccat tgcagcgcat tgccaccacc
     9841 aactcgggtg agttgctttc gttaacgccg gtagagcatg tttgccgact ggtacgtgag
     9901 tcctcgcgcg ttgccgcctg gcgtctgggg ccgagtggct tgagtaccga agatagccga
     9961 cgcatatcct ttcacattcg ttttaaccgt ccgtcatcgc tgtttgctcg ctgctggttg
    10021 ctggtggaag gggaaacgga aacctgggtt atcaatgaac tggcgcgcca gtgtggacac
    10081 catttcgatg ccgaagggat caaggtcatt gagtttgccc agtccgggct aaagccactg
    10141 gttaaatttg cccgccgaat ggggattgaa tggcatgtac tggtcgatgg cgatgaagca
    10201 gggaagaaat atgccgctac ggtacgcagc ctgttgaata acgatcggga agccgaacga
    10261 gaacatttaa cggcgttacc ggcgctggat atggaacatt ttatgtatcg ccagggattt
    10321 tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg taccgatgaa tctgcgcaaa
    10381 attatctcga aagcgatcca tagctcttcc aaacccgatc ttgccattga agtggcaatg
    10441 gaggccggac gtcgtggtgt ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc
    10501 gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa acatcgtggc catttgtggc
    10561 tgaatagcgt cgagcatctc atagcgccga cggtattcag cccgtttttt actggcgatt
    10621 tcggcaatct cttttcgtgc tatctgtgct ggaaggcggt aatggcgttc agcatcacat
    10681 acgccgccaa ccgattccca gaaagcgttg taatcggcat ggatcttgcc ttctttatca
    10741 cgataacgca ggctgcggta aatatgcgtt tcattgctga cggcaataat ctgctctacc
    10801 tgcaaacgtt gggcaaacag acaggccgct tccatcacga ggcgtttggg aaatagcccg
    10861 tggcaggctt tcgtcgcatt ctggatttcc tgatgtggga tttcccattt tgcgccttgc
    10921 agtccgccaa taaacatcgt tcttttcccc tgatattcac acagggtaaa agtgagctct
    10981 gccagaggaa taccttcgct gttgcggaac aggattgtgc tgtcaccttc tttatccatt
    11041 gagatcatca tggtcagctc aagcgtgaac tgctcgccgt ttttgccttc cagtttagcc
    11101 agttgcagcc cgggggtatt caaatataag ctgaattctt ccgccgacat acatccgcgg
    11161 agtaacgcat aatggtaacg taacgcctcc agcaattgct tacggctaag attcgccgca
    11221 aggtaagggc gatgcagacg cacaggcagt cgcggttggc gcgttaacaa cacattgaga
    11281 ttaggccagt gggaaagttc gttcatccac tcaacgctta aacgcggcat aatcaacgag
    11341 cgcagcaaaa atttctggcg aaaactacgg cgatgccaga atttacccgg ccgacactgt
    11401 ccacgtgcca gactaagaaa aagtgacagg ctgctgagag attcagatgg cgtaaaggtc
    11461 cgttcagtta gctgcgacat attcatgaaa tcaatggtta tacatgacgt cgatttcacc
    11521 attgcgtatc ttaaccaaac atcaatagtg tgattactaa cgtaaatttt agggttttgt
    11581 tgatatttcg ttgaagttaa tgacccggat tggcatatgg agtattcaga atatttatga
    11641 aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct ggtgatagtc atcgccggac
    11701 tgattacgtt atggagaatt cttaacgcac ccgtgccgac ttatcagaca ctgattgtgc
    11761 gccccggtga tttacagcaa agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg
    11821 ttgacgtggg cgcgcaggtc agcggtcagt tgaaaactct gtcggtggcg attggcgata
    11881 aagtaaaaaa agaccagctt ttaggggtta ttgatcctga acaggctgaa aaccagatta
    11941 aggaggtcga agcaacgctg atggagctac gtgcgcagcg gcagcaggcg gaagcggagc
    12001 tgaaactggc gcgggtgacg tattcccgtc agcaacgtct ggcacaaacg caggcggttt
    12061 cacagcagga tctcgacacc gccgcgacgg agatggctgt gaaacaggcg caaattggca
    12121 ccattgacgc gcaaatcaag cgcaatcagg cctctctcga tacggctaaa accaatctcg
    12181 attacactcg catcgttgcc ccgatggccg gggaagtcac gcaaatcacc actctgcaag
    12241 gccagacggt gattgccgca caacaagcac cgaacattct gacgctggca gatatgagca
    12301 ccatgctggt aaaagcgcag gtttctgaag cggatgtaat ccacctgaag ccggggcaaa
    12361 aagcctggtt tacggtactt ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg
    12421 tactaccgac gccggaaaag gttaacgacg ctattttcta ttacgcccgt tttgaagtcc
    12481 ccaaccccaa tggtttgctg cggctggata tgactgcgca agtgcatatt cagctcaccg
    12541 atgtgaaaaa tgtgctgacg atccctctgt cggcgttagg cgatccggtt ggcgataatc
    12601 gttataaagt caaattgttg cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg
    12661 cacgtaacga taccgatgtt gagattgtca aagggcttga agcgggcgat gaagtggtga
    12721 ttggtgaggc caaaccagga gctgcacaat gacgcctttg ctcgaattaa aggatattcg
    12781 tcgcagctat cctgccggtg atgagcaggt tgaggtgctg aagggcatca ccctcgatat
    12841 ttatgccggt gagatggtag cgattgttgg cgcttcaggt tccggtaaat cgaccctgat
    12901 gaatattctc ggctgtctgg ataaggccac cagcggcacc tatcgcgtcg ccggtcagga
    12961 tgttgccaca ctggacgccg atgcgctggc gcaactgcgc cgcgagcatt tcggctttat
    13021 tttccagcgt taccatttac tttcgcattt aaccgccgag cagaacgttg aagtacccgc
    13081 cgtctatgct ggtcttgagc ggaaacagcg actgcttcgt gcccaggagt tgctgcaacg
    13141 gctggggctg gaagaccgta cagagtatta tccggcacag ctttcgggtg gtcagcaaca
    13201 gcgcgtcagc atcgcgcggg cattgatgaa cggtggtcag gtaattcttg ccgatgaacc
    13261 aaccggcgca ctggacagcc attctggcga agaggtgatg gcgatcctgc atcagctgcg
    13321 cgatcgtggg catacggtga ttatcgtcac ccacgatccg caggtcgctg ctcaggccga
    13381 gcgggtgatc gaaattcgcg acggcgaaat tgtgcgcaat cctcccgcca ttgaaaaagt
    13441 gaatgttgct ggcgggacgg agcctgttgt caacacggtg tctggctggc ggcagtttgt
    13501 cagcggtttt aacgaggcgc tgacgatggc atggcgggcg ctggcagcga ataaaatgcg
    13561 tactttactg accatgctgg ggattattat cggtattgcg tcggtggttt ccattgtcgt
    13621 ggtgggtgac gccgccaaac aaatggtgct ggcggatatt cgttctattg gtacgaatac
    13681 tattgatgtc tatcccggga aagattttgg cgatgacgat ccgcaatatc agcaggcgct
    13741 gaagtacgac gacttaatcg ccatccaaaa acaaccgtgg gtcgcctcag ccacacctgc
    13801 cgtctcgcaa aacctgcgcc tgcgttataa caatgttgat gttgctgcca gtgccaatgg
    13861 cgtgagcggc gattatttta atgtctatgg catgaccttc agtgaaggaa acacctttaa
    13921 tcaggagcag ctgaacggtc gtgcgcaggt cgtggttctc gacagtaata ctcgccgcca
    13981 gcttttcccc cataaagcag atgtggttgg cgaggtgatt ctggtcggca atatgcccgc
    14041 cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg
    14101 tgtctggcta ccttacagca cgatgtccgg gcgagttatg ggccagtcgt ggctgaactc
    14161 cattactgtc agggtaaaag aaggatttga cagcgccgag gcggaacagc aactcacgcg
    14221 tttactttca ctgcgccacg gaaagaagga tttctttacc tggaacatgg acggcgtctt
    14281 gaaaactgtt gaaaagacca cacgtacttt acaactgttt ctgacgctgg tggcggtgat
    14341 ttcgctggtg gtgggcggta ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg
    14401 gacgcgggaa attggcattc gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca
    14461 gttcctgatc gaagccgtac tggtttgcct ggtcggtggc gcgttgggaa taacactgtc
    14521 actgttaatt gctttcacct tgcagctttt cttacccggc tgggagattg gtttttcacc
    14581 gttggcactg ctgctggcgt ttctctgctc gacggtcacc gggattttat ttggatggtt
    14641 acccgcacga aatgcggcac gactggatcc agtagatgct ctggcacgag agtaattttt
    14701 gagataaaaa tgccagccga tcgggctggc attttgcctt taggatgtac acaatgagac
    14761 agaagagcta tgcgactgcc gcttctactt cgacgggcac aataacactg gcgtgattgc
    14821 cttttggccc ctggtggaca tcaaactgaa cggattgtcc agcttttagc gttctgtaac
    14881 catccatctg aatggtggaa taatgagcga aaatatcttc gccgccgcct tcagggcaga
    14941 tgaaaccaaa ccctttggca ttgttgaacc acttaacagt acccttttcc atgcttcgac
    15001 atccttcgca aatcttatac aagtaagatg gaataaaccg gggtcagaga gggggctgtt
    15061 caaaacctcg ccaactctag aaatacaatt tagagaatta gggcgagccg tcaagcgttt
    15121 gacaggggcc agggggcagg tatgaatcaa aaatttgaag cagttaacgc tattgacagg
    15181 aatgtgacag atgtcgctga tgccaacgat agatgatagt tatctatcat gtggagtaga
    15241 ttggtcaggc aaataagctc ttgtcagcgg cagggcgttc taccgataac cgtaaccgaa
    15301 gatgataact gacaatgggt aaaacgaacg actggctgga ctttgatcaa ctggcggaag
    15361 aaaaagttcg cgacgcgcta aaaccgccat ctatgtataa agtgatatta gtcaatgatg
    15421 attacactcc gatggagttt gttattgacg tgttacaaaa attcttttct tatgatgtag
    15481 aacgtgcaac gcaattgatg ctcgctgttc actaccaggg gaaggccatt tgcggagtct
    15541 ttaccgccga ggttgcagaa accaaagtgg cgatggtgaa caagtacgcg agggagaatg
    15601 agcatccatt gctgtgtacg ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg
    15661 tgcctatgct caatcaagaa ctggaactca gtttaaatat ggctttcgcc agagcgcgcg
    15721 agcaccgtca tgagtttatg accgtcgagc acttgttact ggcgctgctc agtaacccat
    15781 ctgcccggga ggcgctggaa gcgtgttctg tggatttggt tgcgctccgt caggaactgg
    15841 aagcctttat tgaacaaacc acacccgttc tgcctgccag tgaagaggag cgcgacacac
    15901 agccgacgct gagttttcag cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg
    15961 gtcgcaatga ggtaaccggt gcaaacgttc tggtcgctat ctttagcgaa caggagtcgc
    16021 aggcggcata tctgttgcgt aaacatgaag tcagccgtct cgatgtggtg aactttatct
    16081 ctcatggcac gcgtaaagac gagccgacac agtcttctga tcctggcagc cagccaaaca
    16141 gcgaagaaca agctggtggg gaggaacgta tggagaattt cacgacgaac ctgaatcagc
    16201 ttgcgcgcgt gggcggaatc gacccactga ttggtcgtga gaaggagctg gagcgtgcta
    16261 ttcaggttct ctgccgtcgc cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg
    16321 gtaaaaccgc gattgcggaa ggtcttgcct ggcgaattgt tcagggcgat gtgccggaag
    16381 tgatggctga ctgtacgatt tactctctcg atatcggttc tctgttagcg ggcactaaat
    16441 atcgcggcga ctttgaaaaa cgttttaaag cgttgctcaa gcagctggag caggacacta
    16501 acagcatcct gtttattgat gagatccaca ccattatcgg tgcgggtgca gcgtctggtg
    16561 gccaggtcga tgcggctaac ctgatcaaac cgttgctctc cagcggtaaa attcgcgtaa
    16621 ttggttcgac aacctatcag gagttcagca acattttcga gaaagaccgt gctctggcgc
    16681 gtcgcttcca gaaaattgat attactgaac cgtcgatcga agaaactgtt caaatcatca
    16741 atggcctgaa accgaagtat gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc
    16801 gtgcagcggt agagctggcg gtgaaataca ttaacgatcg tcatctgccg gataaagcca
    16861 ttgacgttat cgacgaagcg ggcgctcgcg cacgcctgat gccggtaagc aaacgcaaga
    16921 aaaccgttaa tgtggcggat attgagtccg tggtggcccg tattgcgcgc attccagaga
    16981 agagtgtttc tcagagtgac cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc
    17041 tggtcttcgg tcaggataaa gccattgagg cgctgactga agccattaag atggcgcgtg
    17101 caggtttagg tcacgaacat aaaccggttg gttcgttcct gtttgccggc cctaccgggg
    17161 tcgggaaaac agaggtgacg gtacagcttt cgaaagcgtt gggcattgag ctgctgcgct
    17221 ttgatatgtc cgagtatatg gaacgccata ccgtcagccg tctgattggt gcgcctccgg
    17281 gatacgttgg ttttgatcag ggaggtttgc tgactgatgc ggtcatcaag catccacatg
    17341 cggtgctgtt gctggacgaa atcgagaaag cgcatccgga cgtgttcaat attctgttgc
    17401 aggtgatgga caacggtacg ctgaccgata acaacggacg caaagcggac ttccgtaacg
    17461 tggtgctggt gatgaccacc aacgccgggg tacgtgaaac tgagcgtaaa tccattggtc
    17521 ttatccacca ggataacagc accgatgcga tggaggagat caagaagatc tttacaccgg
    17581 aattccgtaa ccgtctcgac aacattatct ggtttgatca tctgtcaacc gacgtgatcc
    17641 atcaggtggt ggataaattc atcgtcgagt tgcaggttca gctggatcag aaaggtgttt
    17701 ctctggaagt gagccaggaa gcgcgtaact ggctggccga gaaaggttac gaccgggcaa
    17761 tgggcgcacg tccgatggcg cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg
    17821 aactgctgtt tggttcgctg gtggacggcg gtcaggtcac cgtcgcgctg gataaagaga
    17881 aaaatgagct gacttacgga ttccagagtg cacaaaagca caaggcggaa gcagcgcatt
    17941 aatctgattg ttaggtaggt tggtcaagtc cgtaatctcg aaagaggtta cggacttttt
    18001 gtttatgggg tggaggaggt tcagaccctt ttttaatgat gatggtaagt tgttgataat
    18061 tagtgctgcg ggaaggtaag gataaaaaag ggtgctgcag gagaatggga tggttttgct
    18121 ttattaacaa cgggctaaac gtgtagtatt tgagttcact gccgtacagg cagcttagaa
    18181 atcgacgggg tgcggtaaaa cctttgcgaa cgcgttcact gccgtacaga cagataaaat
    18241 gcgaaaaaaa agctcgcact ttcgtacgag ctcttcttta aatatggcgg tgaggggggg
    18301 attcgaaccc ccgatacgtt gccgtataca cactttccag gcgtgctcct tcagccactc
    18361 ggacacctca ccaaattgtt ttgctaccaa acctcatggg tggcaacggg gcgctactat
    18421 agggagttgg agtaaaacgg tcaagaagaa ttttaatgat aattattgtt tgctcatact
    18481 gtaaacaagt tgtgcagtat atctacatcg agacaagtta cggacttata cttccaaagt
    18541 acttcataca tatcacaaaa taaaaaggcc ggttaaaccg accttttact cgttctttct
    18601 cttcgcccat caggcggtaa aacaatcagc gactacggaa gacaatgcgg cctttgctca
    18661 ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt caggatgcgg atgtagtttt
    18721 tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga
    18781 acatggtatt aggcaacgtt tcaagaacgg taccttgcat ttcaatattg tcttctttgg
    18841 ccatctaatc ctctggggta tcactaccgt aatttgaacc ggcaagataa tgccgaagtt
    18901 ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa caggttcggc acattactcc
    18961 gaaaacacac ggctaagccg caccaaaagc gcaacgtata agggagcggt gagataaacg
    19021 atgggcgtta cctgacgcga aaaaatcctt atcggcagcg gggtaatgag cgtaaccaac
    19081 tctgcgaccg caattataac actctgggga gaaatgtgcc gaaaacattc attcttgtgg
    19141 tgaaaacaag catcgtggta cccagaaatt attcggcaat cgtccgaggc gcatttgatt
    19201 gagataatta aggtaatccc ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc
    19261 gttaaggacc tggcagtcga taagcttacc gccatgaccg ataaattcct cacagaatac
    19321 cagaagcgcc gttttagacg cattttccat ccggctgaac atggactcgc cacaaaatag
    19381 cgttccctgg gccacgccgt acataccgcc gacaagctca tcttcacgcc agacttcaat
    19441 ggagtgggca tggccgagtt cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat
    19501 ccaggttcct tcttcgcgat cgctggcaca gccttcaatg acctgaccaa aagcgtaatt
    19561 catcgtgaca cgatagggcg agcgtttatg aaatcgcttc atactacggc tgatatgcag
    19621 tgattctggc catagcaccg cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg
    19681 agaaaaccac ggaaaaatac cacgctggta agccattaac aggcgcgcag ggctaagatc
    19741 gcccccaagt gccagcaggc cgttaggctc acgtaatgcg ccttccgggg aagggaaggc
    19801 tattgaatgg cgagaaagct gaaccaggcg catgaccgca aaactccacg caagtcggat
    19861 cgttcaataa tagcttacaa accctgcttg aactggtaat aacgcccctg tctggcaagc
    19921 agctctgcgt gagtaccttg ctcaataatt tgcccgttgt ccatcactat tatttgttgg
    19981 aaacgagaga gtccgcgaag tcgatgggtg accattaaca ccgttttctc acgcatcatt
    20041 tctgcaagca attcaaggat ctggctttcg gttgtggcat ctaagccttc ggtaggttca
    20101 tccagcaaca ccagtggcgc atcatgtaac agcgcacggg cgatagccag acggcgcagt
    20161 tcaccaccgg agagctggcg tccgccttca cctaaccaac tgttgagacc tgcatcctcg
    20221 agcagcttct ccagaccaac gcgacgcaag ctctccgcca gagcctcatc actactgcca
    20281 ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa acagatgcac tcgctgagga
    20341 acaacgctga tggtctgtcg tagagccgct tcattcaggc tggctatggg gctatcgtta
    20401 agcaaaatct ctccctgttg cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat
    20461 ttgccgcatc cggttcgccc gagaatcgca atatgttccc cggcgtttac ctgaagagaa
    20521 atccctttaa gtgcctgttg ggattgctcc ggataagtga actgaacatc ccgtaatgtc
    20581 agcgaaacgc gatcggcaac acgagtttgg gtatcaggga aggtgacttc cggtttttga
    20641 tccgttaaat cagtgatccg aacggcagag gcaatgactt gccccagatg ctgaaatgca
    20701 cccgttactg gtgccagtgc ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt
    20761 aacgcgccgg gttgagcatt gccgccaacg ccgccagacg ccatccacag catcaggatc
    20821 accgctaacg cgccaatgag cagcattatc gcttgcgaca atgcggtcag ttcagattga
    20881 cggcgttgcg cttccagcca ttgaatttct gtattctcta gttgcgtgcg ataacgatcg
    20941 ctggcgccaa aaatggtcag ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga
    21001 tactgtccgc gaagatgagt cagattttgc ccggtgcttt ttcccgcacg ataaaacagc
    21061 ggtggcatca ggaaaagcgt cagtaacata atgccgccca gcctaaaggc gagggtgaaa
    21121 tcaaggaaac ttaacccgat tgtcaccacc ataatcacca caaaagcgcc caccagcggc
    21181 gagataacgc gcagataaag atgatcgagc gtatcaacat ccgccaccac gcgattgagc
    21241 aattcgccct gacgatagcg cgccagtccg gcaggggaga ggggcagcaa tttgctgaag
    21301 gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt cgtgacttac cagacgttca
    21361 aaataacgcc cggcagtacg ggtgattgct gcgccacgca cgcccgcagc gggtagcata
    21421 tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg aggccgagag gaaccagccg
    21481 gaaagtgtca acagaccgat actggcgagc agcgtgacaa ttgccagcac gataccaaga
    21541 cttaacatcc atttatgacg tttatacagt gccagatagg gtagcaaagc gcgcatttaa
    21601 atctcctcct gacgatgggc cagtaatgtg gcgaatgggc caccagccac acttaattcc
    21661 gcgtaacgtc cttgctcaat aatccggcca tcctgcataa cccaaatgac atcccagtca
    21721 gcaagatctt ctaactggtg ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc
    21781 agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc tggcagcggg ttcatccaac
    21841 agtaatagcg aacagggatt tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc
    21901 acggaaaggc gggcagcctg gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt
    21961 agaaactcgc tgacccaggc gttatccagc gctgcttgta attcttgttc gctggcatca
    22021 ggtcgcgcca gtagtacgtt atcccgcaat gttgctgccg gtaattgtgg gttttgccca
    22081 acccaggaga gatgtttacg ccatgattct ggtgataaat cgcgtaattc tatcccgttg
    22141 attcgtaatg atccctgata tgagagaaaa ccagaaagcg cgttcagcag tgagctttta
    22201 cctgaaccgc tgcgaccaac caacactgca cgttggcctg ctggcaaagt aaagttcagc
    22261 ggtccggcca gcgttttacc ttccggcgac gtgataaaca gatcctcggc ctcaatggtc
    22321 accggatcgg tcgatgctaa ttccacctcg ccacgctgcg gatgggcgag cggggtttcc
    22381 ataaacgttt tcagactgtc agctgcacca acagcctggg ctttagcatg ataaaacgta
    22441 ccgagatcgc gcaatggctg gaaaaactct ggcgcaagga tcagggccag aaaacccgca
    22501 gccagcgtca caccggtatc gtagtgacca aaatccagct cgccgagata ggaaaaacca
    22561 aagtagaccg ccaccagagc aattgacagc gaggtaaaaa attcgagaat gccggaggat
    22621 aaaaacgcca gccgtagcac ttccattgtc cgttggcgga aatcttccga agcagaacga
    22681 atactttcaa tttcagcttc accacgacca aaaatacgca atgtttccat gccgcgcagg
    22741 cgatcgagga aatgcccact taagcgagca agagcgagaa agttacgccg gttagcatcg
    22801 gcagccccca tcccaaccag cgccataaac aacggaatta acggtgctgt gcccagcaga
    22861 atgagcgccg cagcccagtt agaggggaag atcgccacca caatcagcaa cggcaccgac
    22921 actgccagcg ccatttgcgg caggtagcgt gcatagtaat catgcatatc gtcaatttgc
    22981 tcgagtacca acgtcgccca gctccccgca ggtttaccct gaatccacgc tggccctgct
    23041 tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc ggatatgctg cccggcgtga
    23101 taacccaccc gttcgcgtaa ccagaccacc catgcgcgca gtacaaaggt cagaaccagt
    23161 aacgtaaagg gaagcagcag ggcttcacgg ggaatattct ccataatcat atgttgcaga
    23221 attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga
    23281 cgagaaatat tcagccaacg ttgggagatg acgctttgct gttttaacca gcgggttaac
    23341 tctttttgac gggatttatt cattgcacgc ttagcaggtg agttatcaga attatttgca
    23401 gagcaatgtt acaacgggga aaaaataaag gcgacccata gtcgcatggt gtcgccttct
    23461 ttacttttgt tactgatttg taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt
    23521 ccgcatcaag tgctgccatg cagcctgtac cggccgaagt aatggcctga cgataaatat
    23581 gatccatcac gtcacccgcg gcaaagacgc caggaatgct ggtctgggtg gcattaccat
    23641 gaatacccga ctgcactttg atgtagccgt tttccagttc cagctgccct tcgaaaatgg
    23701 cagtattcgg gctgtgaccg atagcaacaa acagaccagc aacgtcgagt gactcgatgt
    23761 tatcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac acccatttga tcgccggtca
    23821 cttcttccag cgtacggttg gtgtgcagaa tgatgttgcc gttctccact ttatccatca
    23881 gacgcttaat aaggattttt tccgcgcgga aaccgtcacg gcggtgaata agatgcactt
    23941 ccgaagcgat gttagacaga tacagcgcct cttcaaccgc ggtattgccg ccgccgatga
    24001 ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac
    24061 tgcctttaaa ggcttcttca gagggcaggc cgagatagcg tgcagaagct ccggtggcaa
    24121 taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt cagacggaac ggacggtttt
    24181 gcagatccac cttgttgata tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt
    24241 cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt tggatcgcca ggccagtttt
    24301 ccacttccgt ggtggtggtc agttggccgc ctttttccat gccggtaatc agcacaggtt
    24361 gcaggttggc gcgcgccgcg tagacagcag cggtgtatcc cgccgggcct gaacccagga
    24421 taagcagttt actgtgtttg gtcgtgccca tgagatcccc atagttgttg gcagacaatg
    24481 ggcaggattg tagggaattt acagacgtaa aaaaagagta tgacgatttt gttaacaatt
    24541 tgtgcaatcg gcagcatcga taagcaggtc aaattctccc gtcattatca cctctgctac
    24601 ttaaatttcc cgctttataa gccgattaaa tgatgaataa acgcccctgt taatgaatat
    24661 ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa
    24721 acattcgagg aagaaaaaaa cagtattctt atatgcgcat aaccatgcat gtaaatacca
    24781 tgtttaccgt gctagtgaaa tctacgtatg gcgtggacag acgccattcg tgatgtcgat
    24841 agctgccaca aggcaacggt cttctcaccg tagacccagg cattgcgcgc cgtgaatctt
    24901 catgatttcg gtctatcgtg acgggtagcg actctgaaca gtgatgtttc agggtcagac
    24961 aggagtaggg aaggaataca gagagacaat aataatggta gatagcaaga agcgccctgg
    25021 caaagatctc gaccgtatcg atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat
    25081 ttctaacgtc gagctttcta aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt
    25141 gcgtcggctg gaaagacaag ggtttattca gggctatacg gcgctgctga acccccatta
    25201 tctggatgca tcacttctgg tattcgttga gattactctg aatcgtggcg caccggatgt
    25261 gtttgaacaa ttcaataccg ctgtacaaaa acttgaagaa attcaggagt gtcatttagt
    25321 atctggtgat ttcgactacc tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa
    25381 gttgctgggg gaaaccctgc tgcgtctgcc tggcgtcaat gacacacgga catacgttgt
    25441 tatggaagaa gtcaagcaga gtaatcgtct ggttattaag acgcgctaac acggaacagg
    25501 tgcaaaatcg gcgtattttg attacactcc tgttaatcca tacagcaaca gtactggggt
    25561 aacctggtac tgttgtccgt tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct
    25621 ttcttgagcc aggaatacac tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc
    25681 cgccgccttc tggaggcgtt gctgatcctt attgtcctgt ttgccgtctg gttgatggct
    25741 gccttactaa gctttaaccc ttcggacccc agctggtcgc aaacggcctg gcatgaacct
    25801 atccataatt taggtgggat gcccggtgcg tggttggcag atacgctgtt ctttattttt
    25861 ggcgtgatgg cttacaccat tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt
    25921 catcagtcca gcgacgaata cattgattat tttgccgttt cgctacgcat cattggcgtt
    25981 ttggcgctca tccttacctc ctgtggtctg gcggcaatca acgctgacga tatctggtat
    26041 tttgcctccg gtggcgtcat tggcagctta ctaagtacta cgctacaacc actgctacac
    26101 agtagcgggg gaactattgc gctgctctgc gtttgggctg cgggcctgac gctgttcacc
    26161 ggttggtcat gggtgaccat tgctgaaaaa ctcggcggct ggattttaaa cattctcacc
    26221 ttcgccagta atcgtacccg tcgcgatgat acctgggtcg atgaagatga atatgaagat
    26281 gacgaagagt atgaagatga aaaccacggc aaacagcatg aatcacgccg tgcccgtatt
    26341 cttcgcggcg cgctagcgcg tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg
    26401 cggcaaacag acgctgcgtt gttctccggt aagcggatgg atgatgacga agagattacc
    26461 tacactgcac gcggtgtggc tgccgacccg gacgacgtcc tattttcggg caatcgtgca
    26521 acgcagccag aatatgacga atacgatcca ttattaaacg gtgcgccaat taccgaacct
    26581 gtcgctgtag cagctgctgc taccacggcg acacaaagct gggctgcgcc ggttgaacct
    26641 gtgactcaga cgccgcctgt tgcctctgtt gatgttccac cctcgcaacc tacagtagcc
    26701 tggcagcctg taccgggtcc acaaacggga gagccggtta ttgctcctgc accggaaggt
    26761 tacccacagc agtcacaata tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa
    26821 ccagtacagc cgcagcagtc gtattatgca cctgcagctg aacaacctgc gcaacaacct
    26881 tactacgctc ctgcggcgga acaacctgtt cagcaaccgt attacgcaac tgcgccggaa
    26941 cagcctgcac aacagccgta ttacgcccct gcgccagaac aaccggtggc aggtaacgcc
    27001 tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca gactgagcaa
    27061 acttatcagc aaccagccgc tcaggagccg ttgtaccaac agccgcaatc cgttgaacag
    27121 cagcctgttg tggagcctga acccgttgta gaagagacaa aacccgcgcg tccgccgctt
    27181 tactattttg aagaagtgga agagaagcga gcccgtgaac gtgaacaact tgcggcctgg
    27241 tatcaaccga ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc gttgaaagcg
    27301 ccttctgttg cagcagtacc tccagtagaa gccgctgccg ctgtttctcc gctggcatct
    27361 ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc
    27421 agtctggcaa atagcggtgg accgcgtccg caagtcaaag aggggattgg cccgcagttg
    27481 ccacgaccga aacgtatccg cgtgccaact cgtcgtgaac tggcgtctta cggtattaag
    27541 ctgccctcac agcgtgcggc ggaagagaaa gcccgtgaag cccagcgcaa tcagtatgat
    27601 tctggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga actggcacgt
    27661 cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga tgtgcccgta
    27721 aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt tgcgcaaact
    27781 caacaacaac gttattccgg cgaacaaccg gctggggcga atccgttctc gctggatgat
    27841 tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc gttgtttacg
    27901 ccaattgttg aacctgtaca gcagccgcaa caaccggttg caccgcagca gcagtatcag
    27961 cagccgcaac aaccagttcc gccacagcag caatatcagc agccgcaaca gccggttgcg
    28021 ccgcagccgc agtatcagca gccacaacag caggttgcgc cgcagccaca atatcagcag
    28081 ccgcaacaac cggttgcgcc acagccgcaa tatcagcagc cgcaacaacc agttgctccg
    28141 cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc agcagcagga taccctgctt
    28201 catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc ataaaccgac gacgccgctg
    28261 ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga tacctttgcg
    28321 cttgaacaaa tggctcgcct ggtggaagcg cgtctggctg atttccgtat taaagccgat
    28381 gtcgtcaatt actctccggg gccggttatc actcgctttg aacttaacct ggctcctggc
    28441 gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg cccgttcact ttcgacggtg
    28501 gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga gttaccaaat
    28561 aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt ccgcgataat
    28621 ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt ggttgccgat
    28681 ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa atctgtcggt
    28741 gtgaacgcga tgatcctgag catgctttat aaagcacagc cagaagatgt gcgtttcatc
    28801 atgatcgacc cgaaaatgct ggagctttcg gtttatgaag gcattccgca tctgttaacg
    28861 gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt taacgagatg
    28921 gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa
    28981 aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg gaagccgggt
    29041 gacagtatgg atgcccagca tccggtgctg aaaaaagaac catacattgt ggtgttggtt
    29101 gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct gatagcacgt
    29161 ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaactca gcgtccatcg
    29221 gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc ctttaccgta
    29281 tccagtaaga ttgactcacg taccattctt gatcaggctg gcgcggaatc actgctgggg
    29341 atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt ccatggtgct
    29401 tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca
    29461 cagtatgttg atggcatcac ttccgacagt gaaagcgaag gtggcgcggg cggtttcgat
    29521 ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc
    29581 aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg tgcagcgcgt
    29641 attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt
    29701 gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt gccggataag gcgcggtagc
    29761 gtcgcatccg gcactctatc aactgaaaat tcagtatttt cttctttcct caagctgatt
    29821 attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa tttgaggaat
    29881 aatgatgaaa aaaattgcca tcacctgtgc attactctca agcttagtag caagcagcgt
    29941 ttgggctgat gccgcaagcg atctgaaaag ccgcctggat aaagtcagca gcttccacgc
    30001 cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga
    30061 tctgtgggtg aaacgtccaa acttattcaa ctggcatatg acacaacctg atgaaagcat
    30121 tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc aagctacggc
    30181 aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc gcaaccagtc
    30241 cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc tgacgccgaa
    30301 agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg gcacaatcca
    30361 tcagtttagc gcggtggagc aggacgatca gcgcagcagt tatcaactga aatcccagca
    30421 aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga
    30481 tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt tcggataata
    30541 cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat atcggccagc
    30601 aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg catttacatt
    30661 ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc
    30721 gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg aaagagattc
    30781 gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact attctttttg
    30841 ttgacgaagt gcaccgattc aacaaaagcc agcaggatgc atttctgcca catattgaag
    30901 acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg
    30961 cactgctttc ccgtgcccgt gtctatctgt tgaaatccct gggtacagag gatattgagc
    31021 aagtactaac tcaggcgatg gaagacaaga cccgtggtta tggtggtcag gatattgttc
    31081 tgccagatga aacacgacgc gccattgctg aactggtgaa tggcgacgcg cgccgggcgt
    31141 taaatacgct ggaaatgatg gcggatatgg ccgaagtcaa tgatagcggt aagcgggtcc
    31201 tgaagcctga attattgacc gaaatcgccg gtgagcgaag cgcccgcttt gataacaaag
    31261 gcgatcgctt ttacgatctc atttccgcac tgcataagtc ggtacgtggt agcgcacccg
    31321 atgcggcgct ctactggtat gcgcgaatta ttaccgctgg tggcgatccg ttatatgtcg
    31381 cgcgtcgttg cctggcaatt gcttccgaag atgtcggcaa tgccgaccca cgggcgatgc
    31441 aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg
    31501 ccattgctca ggcgattgtt tacctggcct gcgcgccaaa aagcaacgct gtctacactg
    31561 cgtttaaagc tgcgctggcg gatgctcgcg aacgtccgga ttatgacgtg ccggttcatt
    31621 tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg
    31681 ctcatgatga agcaaacgct tatgctgccg gtgaggttta cttcccgccg gaaatagcac
    31741 aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggc gaaaagctcg
    31801 cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt
    31861 gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct cccatttaat
    31921 tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag ccagacgcag
    31981 tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg ggcgctcttg
    32041 aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag cgtaactccc
    32101 gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg
    32161 aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat gctttacagg
    32221 ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc tgcagatgaa gtgccggtag
    32281 gtaaagacga aaatgacaac gtcgaagtca gccgctgggg caccccgcgt gagtttgact
    32341 ttgaagttcg tgaccatgtg acgctgggtg aaatgcactc tggcctcgac tttgcagctg
    32401 cagttaagct gactggttcc cgctttgtgg taatgaaagg gcagattgct cgcatgcacc
    32461 gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac agtgagaact
    32521 atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg ccgaaatttg
    32581 ctggcgatct gtttcatact cgtccgctgg aagaagaagc agacaccagt aactatgcgc
    32641 tgatcccaac ggcagaagtt ccgctgacta acctggtgcg cggtgaaatc atcgatgaag
    32701 atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa gccggttcat
    32761 atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc
    32821 agatcgtgcg cccggaagac tcaatggcgg cgctggaaga gatgactggt catgcagaaa
    32881 aagtcctgca gttgttgggc ctgccgtacc gtaaaatcat cctttgcact ggcgacatgg
    32941 gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag aacacctacc
    33001 gtgagatctc ttcctgctcc aacgtttggg atttccaggc gcgtcgtatg caggcacgct
    33061 gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt tctggactgg
    33121 ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat ggtcgtattg
    33181 aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc taatacccaa
    33241 tttttctgaa tctaaaaagc gcctgcgggc gctttaagta gaagtttaat tatttgaaaa
    33301 taaatggatt ttatttttaa gtgtccatat aacgtccatc tttgaatgca aaaagcccac
    33361 agcattctat gctatgggct ttgtttttgg tgtcctcatg agtgagggaa ccaaaaagaa
    33421 aacccgcagt ttttacgctg cgggtttgtt gttcatgtct gtgagatagg gtgccttatc
    33481 gaccttaccc tggcaaccga ttgacggggg attgctcccc cgtcgcggtt tccttactgc
    33541 ttacactgta agaacgccgc aaactccgct ccccagaagc tcatccgtat ttcacacagc
    33601 gaaccgtgca gcatccagat tatgaggatt accgtcacgc agaacgtgat ggccgtaagc
    33661 gatttttgcg acatagcgct tgctcctttg ttggagaggc gctaacctat cacttgctta
    33721 aggtagatat gttagggcct cggttaaaca gagatgtttt ccggggcctt tccacatccg
    33781 gccttcgggt attccctccg accatcagcc gaaaggcacc cgcgcgtaat ctatcgcttt
    33841 tttgttactc cggcaattct gcctgttaat tctgagataa aggcaaactc atctgattgt
    33901 ttcccctgtg tgaagctggc aactcatgcc acgggatacc ttctgaagag tgaacgccgg
    33961 gggcgtgttt cgatgtgaat ttatggaaag cttccagtgt tgagaaactt atgccgcatt
    34021 ccaggttgtt acactggtaa tattttttcc gtacggtgtt tgaatcattt tccgaacgac
    34081 tggtgcggat acgggcagat gcgccacaaa gcggacaacg gaacatagcg acctccctta
    34141 acgtggtgct gccgctattc taagttgctc actctgtttc tgctatccat tccgggattt
    34201 ttgcttcaag ctcaagctgc gtggtaaagc cgctgttatc aatggtgtgc tcggcttttg
    34261 caataatcca gtcctgatta tcaatctcgc ttttaaatcc tgttaccgtg ccatgcattt
    34321 cggggtagag ttctgcacgt ccacgtgcca gcgtgatgga aaatgatgcg gctccgcgtt
    34381 gtagctgctg ccactttgcc gccgctgcgc gtcttgctgc ctgctcgttc tgataagtct
    34441 tgcgtaacac aaacacattg ccttccgcac cttccatata atcaccttca cggctgctgc
    34501 ttttctcctt tttgggtttt ggcggtttac ggcgttttac gctgaccttt ttctttttcc
    34561 cgtaattaag atcaagccag taggcgcgta cccccgtata cgcctcgcgg tcagcaatac
    34621 ggaactgatg gcgatcgccg ctgctgcgtg taatggcgaa cgagggcaac ggctggccct
    34681 gtgcgttcac gccaccaccg ggcatgatga ataacagatt gccgcttttt accgtggtga
    34741 ttgcgcccag catttccgcc atgcgcgtaa ggaaggacat atcgctttct tcggtctggt
    34801 cggcgtggtc gatttcgata tccatcagca tttcgctgat ttgcggtttc agaccatacc
    34861 gatgagcgat ggcggatacc acacgctcaa cggtcacatc atgccaggac acctcacgtt
    34921 taacgttaaa ttcatcccga aaatctgcgc ttctggctga aacagtcagc ctgtccggcg
    34981 gtccttcgtg agcgatttca tcaacaatgt aagtgccttt ttctgtcagc ggttcccctt
    35041 tccagccaat gagaaccgtc agacgcgcgc cccgtggcgg tagctgcaac tggccatcgg
    35101 catcatccag cgtgatggtg agctggtccg cctcaaatcc ccggttgtcg gtcagtgaca
    35161 ggctcatcag gcgttctgcc acgccggaca gcgttttacc ctcggcgaga atatcaaaat
    35221 ccggcatttt cacggggtct gtgccctgac tgagcaattg catggtggtg tcggtcatct
    35281 gctccctccc tgtgtggcat ggtcgcatgt gcgtgcggag ggggttactg ctttttgttg
    35341 tcgccgtggc gggagaacgg cgcaggggtg agattacgcg cgtggtgggt gatgattgtt
    35401 gccgaatcat ttaacggata caaggggctg aagctatgag tgaaactcgt tttcatggtg
    35461 cccgtgttac ggaaaatacc gacctggtaa cagcgattaa cgatgttgat tccagcgtta
    35521 tcggtatcgt ggcaacggcg gatgatgcgg acgcgaagct gttcccgctg aacaagccca
    35581 cactgctgac ccgcgtcaat gacgtgctgg gaaaatgcgg gacaacgggg acgctttatc
    35641 gtgcgcttaa ggccatcgca gaccaggtga gcacaaaggt gatcgtcgtt cgcgtggctg
    35701 aacacaaaga agaagacgga aagacgcagg atcaactggt tatcggtggt tctgaatctg
    35761 acggcagcta tacggggatg tatgcgctgc ttgttgcaga gcaggatgaa agcatcggat
    35821 accgtccgcg tattctggcc gcgccggagc tggacacgga ggcggtgaca aaatccctgt
    35881 gcgtgattgc gggtaaactg cgcgcgtttg tgtatgcctc atgtcatggc tgtgacacga
    35941 tggctgatgc gattacctac cgccagaaat tcaacgaacg tgaggtgatg ctcttatggc
    36001 cggacttcat cgcctacaac ccgaaaagtg gcaaaaacga aacgttcccc gcgcctgcct
    36061 atgcgtgcgg ccttcgtgcg tacattgacc atgagcaggg ctggcacaaa tcactgtcca
    36121 acgttccggt taaaaatgtg ctggggatgt cgaggcatgt gttctggtcg ttgcaggccg
    36181 aagacagcga tgccaacagc ctcaacaaca aagaaatcac gaccattatt cgtcgcaacg
    36241 ggttccgctt ctggggcaac cgcacaccgg aaacgaacgc ctacatcttt gaggtgtata
    36301 cccgaaccgc acaggtgctg gctgattcaa ttgcggaagc gcagtttgaa accatcgaca
    36361 gtccactgac gcctgcgaac gtgaaggatg ttatcagcgc catcagggca aaactggatt
    36421 cgctggttac tgccgggaaa ctgattggcg cggagtgctg gtatgacgtg gtggataacg
    36481 gcaccacgga tttacgtcag gggcgtgtgc gtattcgcta caaatatacg cccgttcccc
    36541 cgctggaaga catggagctt taccagacgt ttactgatga attctttggt cccgcatttg
    36601 cggtgctggg aggtgcctga tggctgtacc aaaacatctt cgctttttta cgttgtttgt
    36661 ggatggtgaa aacgaagtgg gtaaagtgac atccgtcact ctgcctaagc tgacgcgcaa
    36721 aaccgacagc taccggggtg gtggcatgat gggggcagta agtattgatc tcggtctgga
    36781 cgactccgcg cttgatgcga gctttgtcat ggggggcgca gttcgtgagc tgttccttaa
    36841 gtatggcggc acgattgacg gcacgctgct gcgttttgcg ggtgaatact acaccgatgc
    36901 agaaagcgac ctgtatgaag tcgaaatgcg cggacgtgtg acggaaattg atatggggga
    36961 agccaaacag ggcgaagcca catcacacac ttacgccatt aaaaacacct actacaagct
    37021 gagcgttaac gatcgcccgt tgtgggagat tgacctgctg aacttcattt accggaagga
    37081 cggcaaggac attgtgcccg atcgcatccg ttccgcgctt gggcttggct gataagtaat
    37141 atgcaggcgg cgcagtgcgt cgcctctgac tgaaaggagt ttcctgatga aagagacgaa
    37201 aaacatcgat accgaaaaca cggtcgttgc tgacactgtg aaagaaacca gtgagcgtgg
    37261 cgtaaaactt acccaaccaa ttgagcgagg cggcgaaaaa atcacgtatg tggagatcac
    37321 cggagctatt gagcaggctg gatctctgcg agatttgtcg ctgtctgatg tgctgaatct
    37381 gaaagcggaa tccatgttta cgctgctgtc tcgcgtgaca tcaccgcgac t
//
LOCUS       DAZFDP010000028        36466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFDP010000028 DAZFDP010000000
VERSION     DAZFDP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..2893
                     /gene="ycgV"
                     /locus_tag="ACHJ1G_001562"
     CDS             8..2893
                     /gene="ycgV"
                     /locus_tag="ACHJ1G_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4763437.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(2993..4912)
                     /gene="dhaR"
                     /locus_tag="ACHJ1G_001563"
     CDS             complement(2993..4912)
                     /gene="dhaR"
                     /locus_tag="ACHJ1G_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO4763438.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5140..6210
                     /gene="dhaK"
                     /locus_tag="ACHJ1G_001564"
     CDS             5140..6210
                     /gene="dhaK"
                     /locus_tag="ACHJ1G_001564"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO4763439.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6221..6853
                     /gene="dhaL"
                     /locus_tag="ACHJ1G_001565"
     CDS             6221..6853
                     /gene="dhaL"
                     /locus_tag="ACHJ1G_001565"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO4763440.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            6864..8282
                     /gene="dhaM"
                     /locus_tag="ACHJ1G_001566"
     CDS             6864..8282
                     /gene="dhaM"
                     /locus_tag="ACHJ1G_001566"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO4763441.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8602..10299
                     /gene="treA"
                     /locus_tag="ACHJ1G_001567"
     CDS             8602..10299
                     /gene="treA"
                     /locus_tag="ACHJ1G_001567"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4763442.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10378..10818)
                     /gene="ycgY"
                     /locus_tag="ACHJ1G_001568"
     CDS             complement(10378..10818)
                     /gene="ycgY"
                     /locus_tag="ACHJ1G_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO4763443.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(10996..11250)
                     /gene="ymgE"
                     /locus_tag="ACHJ1G_001569"
     CDS             complement(10996..11250)
                     /gene="ymgE"
                     /locus_tag="ACHJ1G_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4763444.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            11451..12185
                     /gene="ycgR"
                     /locus_tag="ACHJ1G_001570"
     CDS             11451..12185
                     /gene="ycgR"
                     /locus_tag="ACHJ1G_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO4763445.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(12187..12798)
                     /gene="emtA"
                     /locus_tag="ACHJ1G_001571"
     CDS             complement(12187..12798)
                     /gene="emtA"
                     /locus_tag="ACHJ1G_001571"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4763446.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            12898..13812
                     /gene="ldcA"
                     /locus_tag="ACHJ1G_001572"
     CDS             12898..13812
                     /gene="ldcA"
                     /locus_tag="ACHJ1G_001572"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4763447.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13907..15643
                     /gene="cvrA"
                     /locus_tag="ACHJ1G_001573"
     CDS             13907..15643
                     /gene="cvrA"
                     /locus_tag="ACHJ1G_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4763448.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16029..17099)
                     /gene="dadX"
                     /locus_tag="ACHJ1G_001574"
     CDS             complement(16029..17099)
                     /gene="dadX"
                     /locus_tag="ACHJ1G_001574"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO4763449.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(17109..18407)
                     /gene="dadA"
                     /locus_tag="ACHJ1G_001575"
     CDS             complement(17109..18407)
                     /gene="dadA"
                     /locus_tag="ACHJ1G_001575"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4763450.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            18737..20269
                     /gene="ycgB"
                     /locus_tag="ACHJ1G_001576"
     CDS             18737..20269
                     /gene="ycgB"
                     /locus_tag="ACHJ1G_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4763451.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(20321..21040)
                     /gene="fadR"
                     /locus_tag="ACHJ1G_001577"
     CDS             complement(20321..21040)
                     /gene="fadR"
                     /locus_tag="ACHJ1G_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4763452.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            21262..22803
                     /gene="nhaB"
                     /locus_tag="ACHJ1G_001578"
     CDS             21262..22803
                     /gene="nhaB"
                     /locus_tag="ACHJ1G_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4763453.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            22949..23479
                     /gene="dsbB"
                     /locus_tag="ACHJ1G_001579"
     CDS             22949..23479
                     /gene="dsbB"
                     /locus_tag="ACHJ1G_001579"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4763454.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(23525..24792)
                     /gene="umuC"
                     /locus_tag="ACHJ1G_001580"
                     /pseudo
     CDS             complement(23525..24792)
                     /gene="umuC"
                     /locus_tag="ACHJ1G_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(24792..25211)
                     /gene="umuD"
                     /locus_tag="ACHJ1G_001581"
     CDS             complement(24792..25211)
                     /gene="umuD"
                     /locus_tag="ACHJ1G_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4763455.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            25584..26495
                     /gene="hlyE"
                     /locus_tag="ACHJ1G_001582"
     CDS             25584..26495
                     /gene="hlyE"
                     /locus_tag="ACHJ1G_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO4763456.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(26702..27148)
                     /gene="ycgN"
                     /locus_tag="ACHJ1G_001583"
     CDS             complement(26702..27148)
                     /gene="ycgN"
                     /locus_tag="ACHJ1G_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4763457.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(27240..27899)
                     /gene="ycgM"
                     /locus_tag="ACHJ1G_001584"
     CDS             complement(27240..27899)
                     /gene="ycgM"
                     /locus_tag="ACHJ1G_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4763458.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(27971..28264)
                     /locus_tag="ACHJ1G_001585"
     CDS             complement(27971..28264)
                     /locus_tag="ACHJ1G_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4763459.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(28276..28434)
                     /locus_tag="ACHJ1G_001586"
     CDS             complement(28276..28434)
                     /locus_tag="ACHJ1G_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763460.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            28505..28906
                     /gene="pliG"
                     /locus_tag="ACHJ1G_001587"
     CDS             28505..28906
                     /gene="pliG"
                     /locus_tag="ACHJ1G_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4763461.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(29009..29377)
                     /gene="ycgJ"
                     /locus_tag="ACHJ1G_001588"
     CDS             complement(29009..29377)
                     /gene="ycgJ"
                     /locus_tag="ACHJ1G_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4763462.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            29897..30592
                     /gene="minC"
                     /locus_tag="ACHJ1G_001589"
     CDS             29897..30592
                     /gene="minC"
                     /locus_tag="ACHJ1G_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4763463.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            30616..31428
                     /gene="minD"
                     /locus_tag="ACHJ1G_001590"
     CDS             30616..31428
                     /gene="minD"
                     /locus_tag="ACHJ1G_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4763464.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            31432..31698
                     /gene="minE"
                     /locus_tag="ACHJ1G_001591"
     CDS             31432..31698
                     /gene="minE"
                     /locus_tag="ACHJ1G_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4763465.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(32070..32414)
                     /locus_tag="ACHJ1G_001592"
                     /pseudo
     CDS             complement(32070..32414)
                     /locus_tag="ACHJ1G_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(32449..32568)
                     /locus_tag="ACHJ1G_001593"
     CDS             complement(32449..32568)
                     /locus_tag="ACHJ1G_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4763466.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(32529..32714)
                     /gene="ymgJ"
                     /locus_tag="ACHJ1G_001594"
     CDS             complement(32529..32714)
                     /gene="ymgJ"
                     /locus_tag="ACHJ1G_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO4763467.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            32815..32988
                     /gene="ymgI"
                     /locus_tag="ACHJ1G_001595"
     CDS             32815..32988
                     /gene="ymgI"
                     /locus_tag="ACHJ1G_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO4763468.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            32990..33334
                     /gene="ymgG"
                     /locus_tag="ACHJ1G_001596"
     CDS             32990..33334
                     /gene="ymgG"
                     /locus_tag="ACHJ1G_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO4763469.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            33338..33673
                     /gene="ymgD"
                     /locus_tag="ACHJ1G_001597"
     CDS             33338..33673
                     /gene="ymgD"
                     /locus_tag="ACHJ1G_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO4763470.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(33730..36051)
                     /locus_tag="ACHJ1G_001598"
     CDS             complement(33730..36051)
                     /locus_tag="ACHJ1G_001598"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4763471.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(36168..36377)
                     /locus_tag="ACHJ1G_001599"
     CDS             complement(36168..36377)
                     /locus_tag="ACHJ1G_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763472.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         9344 a         9185 c         8737 g         9200 t
ORIGIN      
        1 gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gcagatcgtc tcgctgaatt
       61 aaaaatccgt tcgccctcaa ttcaactgat aaaatttggc gctattggtt tgaatgcaat
      121 tatcttttcc cccctgctga tagctgctga tacaggaagt caatatggca ccaatattac
      181 tattaatgaa ggtgacagaa ttacaggaga taccgccgat ccatcaggaa acctctatgg
      241 tgtaatgacc ccagcaggaa acacgcctgg caatatcaac ctgggtaatg atgtcaccgt
      301 caatgtcaac gacgcctctg gatatgcaaa aggaatcatt attcagggca aaaacagctc
      361 cctgacagct aaccgactca cagtagatgt tgttggtcaa acctctgcca tcggcattaa
      421 cttaattggt gactataccc atgctgactt aggcacaggc agcaccatta agagtaacga
      481 tgacggcatc attattgggc atagctcaac actaacagcc actcaattca ccattgaaaa
      541 ctcgaacggt ataggcctaa ccatcaatga ctatggcacc agtgtcgatc ttggaagcgg
      601 aagtaaaatc acgaccgatg gaagtacagg tgtttatatc ggtggtctca acggcaataa
      661 cgccaatggt gctgcgcgtt ttacggctac agacctgaca atcgatgttc agggctacag
      721 cgccatgggg ataaacgtac agaaaaactc tgttgtcgat ctcggaacaa acagttccat
      781 taaaaccaat ggcgataatg cacacggcct ctggagcttt ggccaggtta gcgcgaatgc
      841 actcactgtt gatgtaactg gagccgcggc caatggcgtc gaagttcgtg gtggtacaac
      901 cactatcggt gcagatagcc atatttcttc cgcgcagggc ggtggcctcg tcaccagtgg
      961 ttcagacgcg acaatcaatt tttctggcac ggcagcgcaa cgaaacagca tcttttccgg
     1021 cggttcttat ggtgcctcgg cccagacggc aacggctgtt gtcaacatgc aaaataccga
     1081 tattacagtt gatcgtaatg gcagtctggc gctgggtttg tgggcgctca gcggcggtag
     1141 aataaccgga gacagtttgg ctatcaccgg cgcggcagga gccaggggaa tttatgccat
     1201 gaccaacagc cagatcgacc tcacgagcga tctggtcatt gatatgagta cacccgacca
     1261 gatggccatt gcaacgcaac atgacgatgg ttatgccgcc agccgcatca acgcctcggg
     1321 tcgtatgctt atcaacggta gcgttctttc caaaggtggg ctaatcaatc tagatatgca
     1381 cccagggtcg gtttggacag gttcctccct cagcgataat gtcaatggcg ggaaactgga
     1441 cgttgcaatg aataacagcg tctggaacgt aacaagtaat tctaatctcg acacgctggc
     1501 gctgagccat tcaactgtcg attttgccag ccacgggtca actgccggca catttgccac
     1561 attaaacgta gagaacctga gcggtaacag tacctttatt atgcgtgctg atgttgttgg
     1621 cgagggtaat ggcgttaata ataaagggga tttattgaat atcagcggaa gtagtgctgg
     1681 taatcacgta ttggctatcc gcaaccaggg cagcgaggcc actacgggaa atgaagttct
     1741 gacagtggta aaaaccactg acggtgcggc ctcgttcagc gcgtcttctc aggttgagtt
     1801 ggggggatat ctgtacgatg tgcgtaaaaa tggcactaac tgggagcttt acgcttccgg
     1861 gacagttccg gaaccgactc ctaatcctga accaacaccg gctcccgctc agcctcccat
     1921 agtcaacccc gatcctacgc ctgaacccga tcctacgcct aaccccactc ccacacctaa
     1981 acccaccacg accgcagatg ctggcggcaa ttatctcaat gtcggttact tattaaacta
     2041 tgttgaaaac cgtacgctga tgcaacgaat gggtgacctg cgaaatcaga gtaaagacgg
     2101 taatatctgg ctgcgcagtt atgggggaag cctggactcc ttcgccagtg gcaaactgag
     2161 cggctttgac atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt
     2221 aatgccgttg tatgtcggtc tgtatattgg ctcaacacat gcatcgccgg actatagcgg
     2281 aggcgacggt accgcacgtt cagactacat gggaatgtat gccagttaca tggcacacaa
     2341 cggtttttac agcgatctcg ttgtaaaagc atcgcgccag aaaaatagtt ttcacgtact
     2401 ggacagtcag aacaacggcg ttaacgccaa cggcactgcg aatggattga gcatctccct
     2461 ggaagccggg cagaggttca acctgacccc tactggttat gggttctata tagagccgca
     2521 aacccagctc acatacagcc accagaatga gatggctatg aaggcgagta atggtctcaa
     2581 tatacatctg aatcactacg aatcgctgct ggggcgtgcc agcatgatac tgggatatga
     2641 catcaccgca ggcaacagcc agctgaatat gtatgtgaag actggagcta tccgcgagtt
     2701 ttcaggggat acagactatc tgttgaacaa ctcccgggag aagtacagtt tcaaaggtaa
     2761 tggctggaat aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctacct
     2821 cgaagcggat tacacgcaga gtaacctctt tgatcagaag caagtcaacg gaggatatcg
     2881 cttcagcttt taagtgcggg atagaccagc ccttaaacga tgctgaaaaa tgttagcgtg
     2941 cgccggaatg aaatatagat cactctgatg tatcagaaat cgtctctgtc tttcataccc
     3001 ggcgcttaaa ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc
     3061 ggccgatccc aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt
     3121 taataattgc ctctttttca acttccgcaa atgacagact ggtggaaagg cgtgtggcgc
     3181 tgacatcatc tgttgcctgc tcggtaaaca gatgttccgg caaatcactg acgcgaatgc
     3241 gcccattatc actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc
     3301 caggccatgc acaggaaacc aggcgagcga gggcatcgtc atcaattttc agccgcgtag
     3361 agaagcgttt ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc
     3421 gcatacgcag aggcgggatg gtaatttcaa atgcatgcaa cgcgtaatac agctggcgac
     3481 taaaacgatt ttgttccacc agcattgcga ggtccgcggt cgttgtggca atcactttga
     3541 catcaactgg tattaaacgc cgcgcatcca gtcgcgtgat aacaccctgc ttgataacct
     3601 gaagcaaagc agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc
     3661 cgccgtgtgc cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac
     3721 caataaattc ttccgccagc gccgcatcac catataactc acaattgacg gcgatataag
     3781 gacctgcagc acgctcgctt tcattatgaa ttgcctgact tagcagtgcc tttcccacgc
     3841 cctcttctcc acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa
     3901 tcaagcggcg ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc
     3961 ctaattgact ggtcatcaac tgccgcatct gttccacagg atggagcaac aaaataaagc
     4021 tggttccctg cgtttctatt atcggtttaa gggttatcac cgcatcaata aactgatgct
     4081 gactttcaaa ggttgcttct acgtgtttga gcggatgagc ctgttttatt gcttgttgca
     4141 atacggcggg taacgttaag agttcagtga ttgcccttcc ctgacttgcc gtcgcgtcaa
     4201 ggcgcaagac ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc
     4261 taatcacgcc atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag
     4321 tttcagcgag caaactgtcc gtcagcagta aatttccgac ctcgcgggcg attgccaacg
     4381 tcaacggcaa atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg
     4441 tcaatcggcc cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct
     4501 gtttgaaatg ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata
     4561 gcgcacaagt accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac
     4621 ttagctgctg caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga
     4681 ggataaacag cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct
     4741 gcccgagcgt cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg
     4801 cctgatgtgg tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg
     4861 ttgcaattaa gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca
     4921 tcccttatga caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt
     4981 caatatggaa cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt
     5041 cttttccctt gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga
     5101 acatcatcca tgccctaccg taattgctgg agcaaaataa tgaaaaaatt gatcaatgat
     5161 gtgcaagacg tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca
     5221 ctgcatcagg atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc
     5281 ctgctgtcgg gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg
     5341 atgctttcgg gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt
     5401 gaatgctcca tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc
     5461 ggcgatattc ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg
     5521 accactgtgg tcattgatga cgacgttgcg gtaaaagaca gtctttatac cgccgggcga
     5581 cgcggcgttg ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc
     5641 gactcactgg acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata
     5701 ggtatcgctc tcggtgcctg taccgttcct gccgcgggca aaccttcttt taccctggcg
     5761 gataatgaga tggagtttgg cgtcggcatt catggtgagc cgggtattga ccgccgctcc
     5821 ttctcttccc ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga aaatggctca
     5881 taccatcgca ctttgcgttt ctgggattat caacaaggca gttggcagga agaacaacaa
     5941 accaaacaac cgctccagtc tggcgatcgg gtgattgcgc tggttaacaa tcttggcgca
     6001 actccgcttt ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg
     6061 ggattgacta tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt
     6121 ttctcaatca ccttactgaa agttgatgac gaaacgctgg cactctggga cgccccggtc
     6181 cacaccccgg cccttaactg gggtaaataa ggagaaagca atgtcactga gcagaactca
     6241 aattgttaac tggctcactc gttgtggcga tattttcagc accgagagcg agtatcttac
     6301 cggactggat cgcgaaattg gcgacgctga ccacgggcta aatatgaacc gaggctttag
     6361 caaagtggtg gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaagaa
     6421 taccggtatg acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt
     6481 ctttatccgc gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta
     6541 tcagatgttc cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga
     6601 taaaaccatg tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca
     6661 aaatctctct gttccggtgg cgctcgaagc tgccagtaga atcgccgaat ccgctgcaca
     6721 aagtacgatt acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat
     6781 tggtcaccag gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc
     6841 cgcaaaagag taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac
     6901 tgggagaagg tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg
     6961 ccattgccgc gggaattgac gatccacaaa atcctattgg taccgatgcc gtcaaagtga
     7021 tggaggccat cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta
     7081 gcgcattatt gagtgctgaa actgcgctgg aattgctggc tcccaagatc gccgcaaaag
     7141 tacgtttgtg tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct
     7201 cgggagcgga tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg
     7261 aacaactggg tttaccgtcc tccgacactg aaatctctga cacatgtcct gcgtacgatg
     7321 aagaagcccg ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg
     7381 cctcccggct ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg
     7441 gcaaatgcgt cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg
     7501 atacgctgcg cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc
     7561 agctggctga agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg
     7621 ttccgcctgt ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa
     7681 aatcaaccct gaccgtggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt
     7741 tattagatct gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg
     7801 caatcttttc tggtcaccat acactgttag atgatccgga actgctggcg gcggcaagcg
     7861 aactccttca gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac
     7921 ttagccagca ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg
     7981 acgatcttct gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa ctcccgcagt
     8041 ttaactcgcc aactattcta ctggcggaga acatttatcc ttccacagta ctgcaactgg
     8101 atccggcggt tgtaaaaggt atctgcctta gcgccggaag tccggtatcc cacagcgccc
     8161 taatcgcccg tgaactgggg attggctgga tttgccagca gggtgagaaa ctgtatgcga
     8221 tacaaccaga agaaacgcta acgctggacg ttaaaacgca acgtttcaac cgtcagggtt
     8281 aagccaccgt tcggatggca tcgttctgat gtcatccggc aatttaccca gcactgatgt
     8341 tttttaacta cctgttatat tttttaccaa tttatgatga tttttcttcg ctaatcgcga
     8401 acccaacgtc gctgactggc taataaacag acgttatttg atcactctat ttcaccgtta
     8461 tttatccctt taattccctt ttctaaaatg cctgacagtt cgcagaatga gatttcgatc
     8521 atgcagctag tgcgatcctg aactaaggtt ttctgatact tgaataccgt tttattcggt
     8581 ttcgccaaag gagaatgatt gatgaaatcc cccgcacctt ctcgcccgca aaaaatggcg
     8641 ttaattccag cctgtatctt tttgtgtttc gctgcgctat cggtgcaggc agaagaaaca
     8701 ccggtaacac cacagccgcc tgatatttta ttagggccgc tgtttaatga tgtgcaaaac
     8761 gccaaacttt ttccggacca aaaaaccttt gccgatgccg tgccgaacag cgatccgctg
     8821 atgatccttg ctgattatcg gatgcagcaa aaccagagcg gatttgatct gcgccatttc
     8881 gttaacgtca atttcaccct gccgaaagag ggcgagaaat atgttccgcc agaggggcag
     8941 tcactgcgcg aacatattga cggactttgg ccggtattaa cgcgttctac cgaaaacacc
     9001 gaaaaatggg attctctgtt accgctgccg gaaccttatg tcgtgccggg cggacgtttt
     9061 cgcgaggtat attactggga cagttacttc accatgttag gacttgccga aagcggtcac
     9121 tgggataaag tcgcggatat ggtggccaat tttgctcatg aaatagacac ttacggtcat
     9181 attcccaacg gcaaccgcag ttactattta agccgctcgc aaccgccctt ctttgccctg
     9241 atggtagagt tactggcgca gcatgaaggc gatgccgcgt tgaagcaata cctgccgcaa
     9301 atgcaaaaag aatatgctta ctggatggac ggtgttgaaa acctgcaagc cggacaacag
     9361 gaaaaacgcg ttgtaaaact tcaggatggt acccttctca accgctactg ggacgatcgc
     9421 gatacgccac gaccagagtc atgggtggaa gatattgcca ccgccaaaag caatccgaat
     9481 cgacctgcca ctgaaattta ccgcgacctg cgctctgccg ctgcgtctgg ctgggatttc
     9541 agctcgcgct ggatggacaa cccgcatcaa ctaaatacct tacgcaccac cagcatcgta
     9601 ccggtcgatc tgaacagcct gatgtttaaa atggaaaaaa tcctcgcccg cgccagcaaa
     9661 gctgccggag ataacgcgat ggcaaaccag tacgaaacgc tggcaaatgc ccgtcaaaaa
     9721 gggatcgaaa aatacatgtg gaacgatcaa caaggctggt atgccgatta cgacctgaaa
     9781 agtcataaag tgcgcaatca gttaaccgcg gccgccctgt tcccgctgta cgtcaatgcg
     9841 gcagcgaaag atcgcgccaa caaaatggcg acggcgacga aaacacatct gctgcaaccc
     9901 ggcggcctga acaccacgtc ggtgaaaagt gggcaacaat gggatgcgcc aaatggctgg
     9961 gcaccgttac agtgggtcgc gacagaagga ttacaaaact acgggcaaaa agaggtggcg
    10021 atggacatta gctggcactt cctgaccaat gttcagcaca cctatgaccg ggagaaaaag
    10081 ctggtggaaa aatatgatgt cagcaccacc ggaacggggg gcggcggtgg cgaatatcca
    10141 ttacaggatg gctttggctg gaccaatggc gtgacgctga aaatgctgga tttgatctgc
    10201 ccgaaagagc aaccgtgtga caatgttccg gcgacgcgtc cgaccgttaa gtcagcaacg
    10261 acgcaaccct caaccaaaga ggcacaaccc acaccttaac cagcgcttac tccgacaacg
    10321 tcattcggct tctccggatg gcgttgtcgg cagataacac catagctatg gtgttttcta
    10381 cacagacggt ttttcaagtc ctggcgggaa gtattgcgca aatgtttgcc atacgtggtt
    10441 gccgaagata gcgtcatttc tacgggtaag tgtttcactt tcatcacccg gtgatttaca
    10501 ccgacgttgc ctatataaag catcgattgc tacctgaata ttgttacggt gttcactcat
    10561 caacgttttt ttgctttctg gagctaaatt aataatcaca taatccagac actccagaga
    10621 atttagcaac caaaaaaaga attcagacca ttcctcttcg cgtaactgaa gcattgccga
    10681 acgggtattt tggcttaatc tgccagaagg attgatcaat tctaaatgtt caataagcgc
    10741 agccaaaacc gcctcactat ccgccccatt tttaacgcct gactggctac tgtctaaaat
    10801 atttattttc tgaatcacac caaaaacaac gaaacaataa ttaaaaaagg attctgttgt
    10861 tgttaccggg aaccgacttt atcgggcatc taagtgctat attgatttgt tccacccgta
    10921 aagcaggtgt cgagtatacg ttctgatgtg aaagcggata gcggcatctg tatcgctatc
    10981 cgccttatga aaatcttatt ctcttcgcag gaggcggaat acgcccagaa cgagaatagc
    11041 tcccaccacc gccaccagga agctgtgcag attaaaacca ctgatggagc cgccaatgcc
    11101 aaacatggtc gccagccagc cgccgaccac cgcaccgact atcccgagaa tacaggtcag
    11161 gaaaaatcca ccaccatcac gccccggcat gattagcttg gcgataatgc cggctatcag
    11221 gccaaaaata atccaggcaa taattcccat gttcaggccc tcgtttcgat gaatcggacg
    11281 caagaaatgc ttacgtcaac gctctaagta taggcaaggg cgggatcgat gctgggcgta
    11341 tgtcggctga tttaagaaca acatgtaaaa aatattcacg catttgtatt aagttttgtt
    11401 aactgtgacc gataaaccaa agacagtttg tcagtcagga gtttttccgc gtgagtcatt
    11461 accatgagca gttcctgaaa caaaatccgt tagccgtcct gggcgtgtta cgcgatttgc
    11521 acaaagccgc aattcctttg cgtctcagtt ggaatggcgg gcagctgatc agcaaaatac
    11581 tggcaataac cccggataaa ctggtgctgg atttcggcag tcaagccgaa gacaacatcg
    11641 ccgtgctaaa ggcacagcac attaccatta ccgccgaaac tcagggtgcg aaagtcgagt
    11701 ttactgttga acaactacag cagagtgaat acttgcagct tccggcattt atcaccgtac
    11761 cgcctcccac cttatggttt gtacaacgac gccgatattt ccgcatctcc gccccactcc
    11821 atccgcctta tttttgccag accaaactgg cggataacag tacgttacgt ttccgcctgt
    11881 atgatttgtc gttaggcggc atgggcgcat tactggaaac agcaaagcct gccgaattac
    11941 aagaaggcat gcgcttcgct cagattgaag tcaacatggg gcaatggggt gtttttcact
    12001 ttgacgccca gttaatctcc atcagcgagc gcaaagtgat tgatggcaag aatgaaacca
    12061 tcaccactcc ccgtctgagc ttccgttttc ttaacgtcag cccgacggtg gagcggcaat
    12121 tacagcggat tattttctct ctcgagcgag aagcccggga aaaagcggac aaagtgcgcg
    12181 actgatttac atcgcgtcca gtgcctgctc aagtttgtag atatagcgcg gagcctgcgg
    12241 cgcaggatga tttcgcgcta cgtgatcgag gaactcgtca gcatccaaat cgttgatttt
    12301 gctgatcgcc tttttccgat ctgacgagaa agtccgtagc agcgcacctg ccccgttagc
    12361 gtatgacacc accagcgcat attgcagtac cttcggatct tcaatgcctg ccagcgggcc
    12421 ggtttccaga atattcaggt aagccgcccc cattgaaata ttacgctctg gatttttcag
    12481 ctcgctggtc gtcggctcac cactccagcc catacggcga taaacatcac gtccggaggt
    12541 tgaagctttt aactgcatca aaccaatggc attcgattta ctcaccgcat tcggattacc
    12601 acccgattcg atagcgataa tcgccgtgat caattgtgga tcgacgcccc aggctgcacc
    12661 ggctttctgg cttattggca tccactgcat cgcacgttgc accggaactt tcgcgttcca
    12721 cggcgggttc gtatagtcat gctttgatga acaacccgct aataacacaa tcaaaaaggc
    12781 aaaccatctc aatttcactt cttctatcct tatagcctgg aatcgccgtt gaggtgacaa
    12841 cggcacacct aagcggcatg atatacattt cgtttctgtt gtcagcaagg aattgccatg
    12901 tctctgtttc acttaattgc cccatcgggt tactgcatta aacagcacgc cgcgctgcgt
    12961 ggtatccagc gccttaccga cgaggggcat caggtcaata acgtcgaggt tattgcccgt
    13021 cgttgtgagc gttttgcggg tacggaaacg gagcgcctgg aagatctcaa ttcccttgcc
    13081 agactgacca cccccaacac catcgtgctg gctgtacgcg gcggttacgg tgccagtcgt
    13141 ttactggcag atattgactg gcaggcactg gttgctcgcc aacaatatga cccgttgctc
    13201 atatgcgggc atagcgattt taccgccatt cagtgcggtc ttctggcgca aggcaatgtc
    13261 atcaccttta gcggccccat gctggtagca aactttggcg cggatgaact gaacgccttt
    13321 accgagcacc atttctggct ggcgttacgc aatgaaacct tcaccattga atggcaaggg
    13381 gaaggcccga catgtcagac cgaaggcacg ttgtggggag gcaatcttgc gatgctgatt
    13441 tcactgattg gtacaccgtg gatgccaaaa attgagaacg gtattctggt gctggaagat
    13501 attaacgagc accctttccg cgtcgaacgt atgctgttgc agctctatca tgcgggaatt
    13561 ttgccacgtc agaaggcgat tattctcggt agctttagcg gcagcacgcc caatgattat
    13621 gacgcaggtt acaacctgga gtcagtgtac gcgtttttgc gttcccgcct gtcgattcca
    13681 ctcattaccg gcctcgattt tggtcatgaa caacgcacgg ttacgctgcc gttgggcgca
    13741 catgccattc tgaataacac ccgggaaggc actcagttga cgattagcgg tcatcctgtt
    13801 cttaaaatgt aaaaaaatca cgcttcaggg ctaagtaaaa cgctgtctct gccgctaata
    13861 tggtagggtt tatataagag acagcgtaat caggagtaac cgaccgttgg atgccacaac
    13921 aataattagc cttttcatct taggatccat ccttgtcacc agcagtatat tacttagttc
    13981 attttcttcc cgtcttggca ttcctattct ggttattttt ctggcgatcg gcatgctggc
    14041 aggagtcgat ggcgtcggcg gtatcccgtt tgataattac cccttcgcct acatggtaag
    14101 taacctggca ctggcgatta ttttgctcga cggcgggatg cgcactcagg ccagttcctt
    14161 tcgtgtggcg ttaggaccgg cactgtcgct ggcgacgctg ggcgtgctta tcacctctgg
    14221 tttaaccggc atgatggcgg cgtggctgtt taatcttgat ttgattgaag gcttattaat
    14281 cggcgctatc gtcggctcta ccgatgctgc agcggtcttt tctctgctgg gtggtaaggg
    14341 gcttaacgaa cgtgttggct cgacgctgga aattgaatcc ggcagtaatg atccaatggc
    14401 ggtctttctg acgattaccc tgattgcgat gatccagcaa catgaaagca gtgtcagttg
    14461 gatgttcgtg gtcgatattc tgcagcaatt tggcctcggg attgtcatcg gacttggtgg
    14521 tggttattta ctgctgcaaa tgattaatcg catcgccctg cccgccggat tatatccatt
    14581 gctggcatta agcggcggta ttttaatttt tgctttaacc acggcgctgg aaggcagcgg
    14641 tattctggct gtttatctgt gcggttttct gctgggtaat cggccgattc gcaaccgcta
    14701 cggcatcctg caaaatttcg acggcctcgc ctggctggcg caaatcgcca tgttcctggt
    14761 gctggggctg ttggttaacc caagcgatct gctgcctatt gccattccgg cgctcatttt
    14821 gtcggcatgg atgatattct tcgcccgtcc cctttcggta tttgctggat tgctcccctt
    14881 ccgcggtttc aatctgcgtg agcgcgtgtt tatcagctgg gtaggattac gcggcgcggt
    14941 gccaatcatc ctggctgtgt tcccgatgat ggcggggctg gagaatgcac gtctgttctt
    15001 taatgtcgcc ttctttgtgg ttctggtttc actgctcttg cagggaacat cactctcgtg
    15061 ggcggctaaa aaagccaaag tggtggttcc gccagtggga cgtccggtgt cacgcgttgg
    15121 cctggatatt catccggaaa atccgtggga gcagtttgtt tatcaattga gtgccgataa
    15181 atggtgcgtg ggcgcggcac tgcgcgattt gcatatgcca aaagagacgc gtattgcggc
    15241 actgtttcgt gataaccagt tgcttcatcc taccggcagc acccgactgc gcgaaggcga
    15301 tgtgttgtgt gtaattggtc gggaacgcga tcttccggcg ctcggtaaac tgttcagcca
    15361 gtcgccgccg gtcgcgctgg atcaacgctt ctttggtgac ttcattctcg aagccagcgc
    15421 caaatatgct gatgttgcgc tgatatatgg cctggaagac gggcgagaat atcgtgataa
    15481 gcagcaaacg ctgggtgaaa ttgtccagca gttgttaggc gcggcaccgg ttgtcggtga
    15541 ccaggtagag tttgccggga tgatctggac ggtggccgag aaagaagata atgaagtgtt
    15601 gaagattggc gtacgggtgg ctgaagagga agctgaatct taaatttaat gaagcatcta
    15661 tcgccggttg cgatgctttg ctgaacgcag cctgagcctg caattgtttg tcgtccataa
    15721 tttaaccttc atttgatgtt gaattgaaca tatcaaaatc aggcaattac acaaatctat
    15781 gtacaggctc catcaagggt aaagcgtgat ttatctgaag tcgagttcga gtcaacaatg
    15841 aagcgtaagc gttttatgta gaacgcctgt gcggtgagtt caggttccgg ggaaatagtg
    15901 tccattcttt taatggacac tatcatgggc aatggctctg tcacattaac aactacagtt
    15961 gctgaccagc cggatggatg attttttcgc aaccagaaga cgctgcctcc gatccggctt
    16021 acaacaagtt acactgtcac aaccgggacg cgtaacgcca gcgcgcacat caactcatag
    16081 cccaccgttc cggcagcggc ggcgacatca tcaattttga tctccttgcc ccacagctca
    16141 accggcgtac caatccccgc ctgcgggcaa ggcgttaaat cgaccgctag catatccatc
    16201 gagacggtcc ccaccgtcat ggtgcgcacg ccgtccacta aaacaggggt accggtaggc
    16261 gcgtggcgcg gataaccgtc ggcgtaccct gcggcgacaa tgccgattcg ctgttcatcg
    16321 cgcgcagtat agcgaccgcc gtagcccaca cgctcacccg cttttagcgt ctggacacca
    16381 ataatctcac tgcttagcgt catcaccgga cgtaatccgg tattggcgat atcacgccac
    16441 tgaccggacg gcgaagcgcc atacaaaata atgccaggcc gaacccagtc aaaatgcgct
    16501 tccgggtgcc acagagtcgc cgccgaattg gacaacgaac gccgacactc cagcccttcc
    16561 gccgcctgct caatacgcgc catcgcgctg gaaattccat caggatgttc cgcttcggca
    16621 aaatgcgaca tcagggtcat ctcgccaaca ttcgccattg cccgcaactg ctgccagacg
    16681 gtaagcacgc gatcgggctg gaagcccaac cgattcatcc cactgttcac tttaagataa
    16741 atatccaacg gtgcttttag ccgcgcattt tgcagtgctt tgagctgcca gttgctgtgt
    16801 acgcaggtgg tcaggcggtg ctggtcataa atctccagat cctgagcatg gaaaaatcct
    16861 tccagcatca ggatcggccc cttccagccg cgctcacgta acgttattgc ctcttccagg
    16921 ttaagtaatg caaagccatc ggtggccccg agcgcgctcc agatacgctc aataccatgc
    16981 ccgtaagcgt tcgcttttac caccgaccag acgcgcgcgt gcggcgcggc ctggcggaca
    17041 atggacagat tctgttttaa tgcctgcaga tcgaggctgg cctgtatcgg acgggtcatc
    17101 tcgtttcctt agctgtgtgc gccatgtaaa tggcccggac gtgatggcgt aaatccacgg
    17161 ctgtagcgcg ctacgcttag atcctcatat gggatcgctg gcgtgcgacc agagagcaga
    17221 tcgcttaaca actgaccgga accgcaagcc atcgtccagc cgagcgtgcc gtgaccggta
    17281 ttcagccaca gatttttaaa gcgtgtacgc ccgacaaccg gcgtaccgtc tggcgtcatc
    17341 gggcgcaaac ccgtccagaa agtcgcctgc tcgacatgac cgccgcgtgg atagagatcg
    17401 cgaaccacca tttccagcgt ttcacgacgc ggttgcaaca gctcggtgtt aaaaccaaca
    17461 atttccgcca ttccgccaac acgaatgcgg ttatcgaaac gggtaatggc gattttgtag
    17521 gtttcatcaa gaatagtgga tactggcgca ccatcttcct gcgcaattgg aatagtcagc
    17581 gagtagcctt tcagcggata caccggaata tcaacaatgc ctttgagcat cgccgtcgag
    17641 taagaaccaa acgccatcac atacgcatcg gccttaatca cttcatcgcc acacttcacg
    17701 ccgtagattt gctcgccgtc gcaaagcagt tggtcaacgg gcgtattaaa gcggaattta
    17761 acccccgcct gctctgccat ccgcgccaga ttctgggtaa acagctggca gtctccggtt
    17821 tcatcattgg gcagttgcag accacccgtc agtttgtgcg ctacttctgc cagcgcgggc
    17881 tccacttccg ccaggcggct ggattccagc agctgatacg gtacgccggc atcttccagc
    17941 acggcgatat cgcgggtcgc attttcatac tgttgttcgg tacggaacag ttgcagcgtc
    18001 ccaccctgac gcccttcata ctgaatattg gtttcggcgc gcaatgcttt caggcaatca
    18061 cggctgtatt ccgccagacg caccatccgc cctttgtttt ccatgtagtg gctggtgtcg
    18121 cagttacgta acatttgcca catccatttc aactgaaact gcgtaccgtc gagacggacc
    18181 gccagcggcg catggcgctg gaacatccat ttgatcgctt ttaaaggcac acctggtgcc
    18241 gcccacggtg cagcatatcc gggggagatt tgcccggcat tcgcggcgct ggtttccagg
    18301 gcggcccccg gctcccgatc aatgacggtg acctcatgtc ctgcctgatt taagtaccag
    18361 gcgctggcaa cgcctaccac accacttccc agtatgacaa ctcgcatagc cactccgata
    18421 acggtagata cagtaaaaga ataataatct aattacatct tgataactca gttgaaaata
    18481 ttattcaaca tacggctttt ttatggtgag ctggctcaca tctccctgac tctgcggcaa
    18541 tatggcgtct ttagtatctc ctgctgaacg ccctttttat ccagcataaa attccgttca
    18601 gaagcggatt agtggcactc tgataataag aaatcgcaaa gaaaaaaatt ctccggcgac
    18661 gcggttttta acaggtgttc tatgcttgaa atgaggtgct ccacacagag agcgccgaca
    18721 acaatgaggt gcgcgtatgg cgacgatcga ttctatgaat aaggacacca cacgtttgag
    18781 cgatggaccc gactggacgt tcgacctgct ggatgtttat ctggcagaga tagaccgggt
    18841 ggcgaaactc taccggctgg atacctaccc gcaccagatt gaagtgataa cctcagaaca
    18901 gatgatggat gcctactcca gcgtcggcat gccaattaac tatccgcact ggtcattcgg
    18961 taaaaagttt atcgaaactg aacggctgta taagcacggt cagcaaggac tggcctatga
    19021 aatcgtcatt aactctaacc cgtgtatcgc ttacctgatg gaagagaaca ccattaccat
    19081 gcaggcgctg gtgatggcac acgcctgcta tgggcataac tctttcttta aaaacaatta
    19141 cttattccgc agttggaccg acgccagttc gattgtcgat tatctgattt ttgcccgtaa
    19201 atatattacc gagtgcgaag aacgttatgg cgttgatgaa gtagaacggc ttctggactc
    19261 gtgccacgcg ctgatgaact acggcgtgga ccggtacaaa cgcccgcaaa aaatctcact
    19321 gcaagaagag aaagcccggc agaaaagtcg cgaagagtat ctacaaagtc aggtcaatat
    19381 gctctggcgt accctgccga agcgcgagga agagaaaacg gttgctgaag cgcgccgcta
    19441 tccgtccgaa ccacaagaaa acctgctcta ttttatggag aaaaatgcgc cactgctgga
    19501 atcatggcag cgtgaaatcc tgcgtattgt gcgtaaggtg agccagtatt tttatccgca
    19561 aaaacagact caggtgatga acgaaggctg ggcgaccttc tggcactata ccatccttaa
    19621 ccatctgtat gatgaaggga aagtaacgga acgttttatg ctggagtttt tgcacagcca
    19681 caccaatgtg gtcttccagc ccccctataa cagcccgtgg tacagcggca tcaacccgta
    19741 tgccctcggg ttcgccatgt tccaggatat taaacggatt tgtcagtcgc caacggaaga
    19801 agacaaatac tggttcccgg atatcgccgg ttccgactgg ctggaaacgc tgcatttcgc
    19861 gatgcgtgat ttcaaagatg agagttttat cagccagttt ctgtcaccga aagtgatgcg
    19921 tgatttccgc ttcttcaccg tgctggatga tgatcggcat aattatctgg agatttccgc
    19981 tattcataat gaagaaggtt atcgggagat ccgtaaccgg ttatcgtcgc aatataactt
    20041 aagtaatctg gagccgaata ttcagatctg gaacgtggat ttgcgcggcg accgttcgct
    20101 gacgctgcgt tacattccac ataatcgcgc accgctggat cgggggcgca aagaagtgct
    20161 gaagcatgtg catcgcctgt ggggatttga tgtgatgctc gaacagcaaa acgaagacgg
    20221 cagcatcgag ttgctggaac gttgcccgcc aagaatggga aatctgtaaa aacaacaaaa
    20281 aacccctcgt ttgaggggtt tgctctttaa acggaaggga ttatcgcccc tgaatggcta
    20341 aatcacccgg cagatttttc tgcatccggt gccaaatctc gccactctca tgcccatagc
    20401 gacgcactgt ttcgtacacc tgatcgtgcg cgccttcact gcacaacgcc gacagtttgt
    20461 ggtagaagcc cagcgccaga ctgcgcgctt ccggattggc gaaatagtga cgaccaatgc
    20521 gcgtatacag ccctttcatc ccgttaagga tcagaccgta aatcgggttg ccggaagcaa
    20581 acgccaggcc gcggaatatg ttgtaatcca gctcggcaaa ggcatcggcg tgatcggcca
    20641 cttcattagc ggtagccagc acttcctgcg ctttatcggg atgctgacga aacgcggtgc
    20701 gaataaaaat agtggaaata ttggtacgca ccgacagcaa attatcaata agctgcggca
    20761 cactttcgtg atccagtcgc gccagtgttt caaggatatt taaaccagag gtttcccaga
    20821 aattattcac cttcgtcggc ttgccatgtt gaatggtcaa ccagccatct cgtgccagac
    20881 gctgtaacac ttcacgtaac gtagtacgcg ttacgccaat taattctgaa agttcacgtt
    20941 ctgcgggcaa aatagtcccg ggagggaagc ggttattcca gatactttca ataatgtact
    21001 cttccgcgaa acccgccggg ctttgcgcct taatgaccat agtgagattt ccataacaca
    21061 gcaaaacaaa gttggactca tcataccaga gggctaacta cgctgatagc agacaaaaag
    21121 aagaaaaaag ggatcaaggc ttcattttcc gcatctcttt ccccttgact atcaagactt
    21181 gcctcactcc tgcctgaccg ttaagctttg cgtttaaaca taaaaacatc attgattatt
    21241 tgtaaggtaa gggaaaccat catggagatc tcctggggcc gcgcgctatg gcgcaatttt
    21301 ttgggccagt cccccgactg gtacaaactc gccctcatca ttttcttaat cgtaaacccg
    21361 ttaattttcc tcatcagccc tttcgtcgcg ggctggttgc tggtagcgga atttattttc
    21421 actctggcga tggccctgaa atgctacccg ctgctccccg gtggtctgtt ggctatcgaa
    21481 gcggtattca tcggcatgac cagcgcggaa cacgtccgtg aagaggtggc ggcaaatctt
    21541 gaagtcttgc tgttattgat gtttatggtg gcgggtatct attttatgaa acagctgttg
    21601 ctgttcatat ttacccgttt gctgttaagc attcgctcca aaatgctgct gtcgctctct
    21661 ttttgcgtgg cggcggcgtt cctctccgcg ttcctcgatg ccttaaccgt cgtggcggtg
    21721 gtgatcagcg ttgcagtcgg tttttatggt atttatcacc gcgtagcctc ttcccgtact
    21781 gaagataccg acctgcaaga cgatagtcat atcgacaagc attacaaagt ggttctggaa
    21841 cagttccgtg gctttctgcg tagcctgatg atgcatgccg gtgtcggcac tgcattaggc
    21901 ggcgtgatga ccatggtggg cgaaccacag aacctgatca tcgctaaagc ggctggctgg
    21961 cattttggcg atttcttcct gcgcatgtcg ccggtgaccg ttccggttct gatttgtggc
    22021 ctgttaacct gcctgctggt agagaagctg cgttggtttg gctacggtga aacgctgccg
    22081 gagaaagtcc gcgaagtgtt gcaacagttt gacgatcaaa gccgccacca gcgtactcgt
    22141 caggataaaa tccgtctgat tgtccaggcg attattggcg tctggctggt gactgcgctg
    22201 gcgttgcatc tggcggaagt tggcttgatt ggtttgtcag tcattattct ggcgacatca
    22261 ttgaccggtg tcaccgatga acatgctatc ggtaaagcct tcaccgaatc tctgccattc
    22321 accgcactgt tgacggtgtt tttctcggta gtcgcggtga ttatcgacca acaactgttt
    22381 tcgccaatta ttcagtttgt gttgcaggca tcggaacatg ctcagctgtc gctgttctat
    22441 attttcaacg gtctgctgtc atccatttcg gataacgtct tcgtggggac gatttatatc
    22501 aacgaagcga aagcggcaat ggaaagtggc gctatcacgt tgaagcaata cgagctgctg
    22561 gcggtcgcca ttaataccgg taccaatctg ccctccgtcg ctacgccgaa cggtcaggct
    22621 gcgttcctgt tcctgctgac ctctgcactc gcgccattga ttcgcctctc ttatggccgc
    22681 atggtgtgga tggccctgcc ttacaccctc gtactgacac tcgtcggctt gctctgcgtc
    22741 gagtttacgc ttgcccctgt aaccgaatgg tttatgcaaa tgggctggat agcaacgctt
    22801 tgataacaac ttaccgggca tataaatgcc cggtttgcct tttcgcccga taattgtcca
    22861 attgcgtcct atttttgctg cctcctggtg gcgcagcgaa tgaattggtt taaactgcgc
    22921 actctatgca tattgcaggg aaatgattat gttgcgattt ttgaaccaat gttcacaagg
    22981 ccggggcgcg tggttgttga tggcgtttac tgctctggca ctggaactga cggcgctgtg
    23041 gttccagcat gtgatgttac tgaaaccttg cgtgctctgt atttatgaac gctgcgcgtt
    23101 attcggcgtt ctgggtgctg cgctgattgg cgcgatcgcc ccgaaaactc cgctgcgtta
    23161 tgtagcgatg gttatctggt tgtatagtgc gttccgcggt gtgcagttaa cttacgagca
    23221 caccatgctt cagctctatc cttcgccgtt tgccacctgt gattttatgg ttcgtttccc
    23281 ggaatggctg ccgctggata aatgggtgcc gcaagtgttt gtcgcctctg gcgattgcgc
    23341 cgagcgtcag tgggattttt taggtatgga aatgccgcag tggctgctcg gtatttttat
    23401 cgcttacctg attgtcgcgg tgctggtggt gatttcccag ccgtttaaag cgaaaaaacg
    23461 tgacctgttc ggtcgctaat ccattcggcg ctcctgcggg agcgcttttt tcctgccgct
    23521 atatttattt gacccgcagt aaatcagaac ttcgcgttgt ataacgtggt gaaagcatgg
    23581 cacgcttcat ctgccattgt tgctggatcc cctgcccggc aaaatagagt gttcctctgc
    23641 cctctttagc attcagtgta tccattaccg tcatcaattg ctcactcccg gggcgcggtg
    23701 cgttgtcatc gaataaattg agctgcgcga ctccctgact gaagaaatcc cccagcatca
    23761 cgcccgcttt ttggtagcga tggcccgctt gccagatggc atccagagat cgcgtagcag
    23821 cgttaatgat atccctgctg tcctgagtgg gcgtcagcag ttttaccgac gcgctattgc
    23881 cgtaataagg ttcattgagc gcaaatggtg acgtcttaat aaatgtggag ataaaccgac
    23941 aatattgatg ctcgctgcga agtttttccg ccgcccgggc agcgtaacta caaatggcct
    24001 gccgcatcga tggataatcc gtgatgcgtt caccaaacga gcgggaacag ataatttcct
    24061 gcttcgtcgg tgcaaactct tccagttgca aacagggttc gccgcgcagt tcacgcaccg
    24121 ttctttcgag cacgacatta aaatgtttac ggataaaccg gatatctgta tccgccaaat
    24181 caagaacggt tttgatcccc atcgcgtcca gttttttgct gatccgccgt ccaatccccc
    24241 agacttcatc cacggggaga gcagacatta atttacgctg gcgttccaga tttgataaat
    24301 ccaccacccc cccgtctgcc gctgccattt ttttgccgca tgattggcaa gcttagccag
    24361 cgttttggtc tgggcgatcc ccacaccaac agtaagatgg gtacgttgta gcaccgttgc
    24421 gcgaatttct ctgccaaaat cagtcagatc gcgacaatta cgcacacctg tcagatcgca
    24481 gaatgcctca tcaatactgt aaatctcgac gcggggcgat agttcttcca gcgtcgacat
    24541 cacccgattg ctcatgtctg cgtaaagctc ataattgctg ctaaagcaaa ccacaccaca
    24601 gcgacgaaac agatcttttt gtttgaacca gggatcgccc attttaacac ccagcgcctt
    24661 tgcctcagcg tttcgggcga taacgcaacc gtcattattc gatagcacaa ccaccggttt
    24721 accccataaa tcagggcgaa acaccgtctc acagctggca taaaacgcgt ttacatcaca
    24781 gagagcaaac atcagcgcat cgccttaacg acgtggatca ccacaccaaa gacatccagc
    24841 gtatcttcgc tactgatggt aatgggcgag tacgcactgt tcatgggaat aagctgtacc
    24901 gtcgggcgta gttgcaattt tttcaccgta aactcgccgt caacagcagc gatgacaata
    24961 tcaccatggc tggcggtaat agcgctatcg acaatcagta aatcaccgtc actaattcca
    25021 ccatcaatca tagaatcacc acttgctttg acgaagtaag tcgcgctggg atgctggatc
    25081 aacagttgat tcagatcgat gcgctgttca acgtaatctg ctgccggtga aggaaagcca
    25141 cactgaacaa gatcgctaaa tagcggaaaa gtcacaattt cgcggagatc cgcaggcttg
    25201 ataaacaaca taataatctg cctgaagcta tactgttttt atatacagta gtctgttctt
    25261 gccagcagat caatactgat tcaggctatc aatatttgtc gctgcataga ctgctgattt
    25321 ttcgttctct tatcctgtgc tcacgcggcc ttctggcgac tacgctcatc cagcagaaat
    25381 gaaaaatatt acccggctaa aaaatagaat agaagcattc gccataatga cattaaacat
    25441 tgtttgatat ttatcatatt aatagaaata aagacattga cgcatcccgc ccggctaact
    25501 atgaattaga tgaagtaaaa tttattaata gttgtaaaac aggagtttca ttacaattta
    25561 tatatttaaa gaggcgaatg attatgactg aaatcgttgc agataaaacg gtagaagtag
    25621 ttaaaaacgc aatcgaaacc gcagatggag cattagatct ttataataaa tatctcgatc
    25681 aggtcatccc ctggcagacc tttgatgaaa ccataaaaga gttaagtcgc tttaaacagg
    25741 agtattcaca ggcagcctcc gttttagtcg gcgatattaa aaccttactt atggatagcc
    25801 aggataagta ttttgaagca acccaaacag tgtatgaatg gtgtggtgtt gcgacgcaat
    25861 tgctcgcagc gtatattttg ctatttgatg agtacaatga gaagaaagca tccgcccaga
    25921 aagacattct cattaaggta ctggatgacg gcatcacgaa gctgaatgaa gcgcaaaaat
    25981 ccctgctggt aagctcacaa agtttcaaca acgcttccgg gaaactgctg gcgttagata
    26041 gccagttaac caatgatttt tcagaaaaaa gcagctattt ccagtcacag gtagataaaa
    26101 tcaggaagga agcgtatgcc ggtgccgcag ccggtgtcgt cgccggtcca tttggattaa
    26161 tcatttccta ttctattgct gcgggcgtag ttgaagggaa actgattcca gaattgaaga
    26221 acaagttaaa atctgtgcag aatttcttta ccaccctgtc taacacggtt aaacaagcga
    26281 ataaagatat cgatgccgcc aaattgaaat taaccaccga aatagccgcc atcggggaga
    26341 taaaaacgga aactgaaaca accagattct atgttgatta tgatgattta atgctttctt
    26401 tgctaaaaga agcggcaaaa aaaatgatta acacctgtaa tgagtatcag aaaagacacg
    26461 gtaaaaagac actctttgag gtacctgaag tctgataagc gattattctc tccatgtact
    26521 caaggtataa ggtttatcac atttaaaaac acagacgttt tttaaagatt gagagttacc
    26581 tgttgataat aaggtatcgg gtaactctca ttatgacaga gcgctttcaa aaacagggct
    26641 acgaaatggc gagcatccag acgtatatta agaaagcccc catcaacatc tattaaattt
    26701 atcactgcgc ccagtcaggt ttatttaaga tatgatcctg ccagtcaatc acttctgatt
    26761 ctttcactgc gatatgacgc acagagatac gttcaccatg cattgccgct ttcgaacccg
    26821 taagtagcgg atgccacgca ggtaaatctt taccttccgc cagcaaccga taagcgcagg
    26881 tcattggcag ccattcgaat gttggcagat tttcacgggt taatttaatg cagtcgggtt
    26941 caaactcgaa acgacgttcg tagttccgac attgacaggt tttaatattg agctggcgac
    27001 aggcgacgtt agtgaagtag atttcgtcgg tgtcttcatc catcagttta tgcaggcaac
    27061 actgaccgca accatcacac aacgactccc attccgcatc gctcatttcg tccagggttt
    27121 tactttgcca gaaaggtaca tcgctcatca ggtgttccgc cattacgtta aaacgcacct
    27181 tataaccagt ctggcacagc gatgcaagtt ttgccgccgc tttcaggcgg caaaaagtat
    27241 tacaaaacgc gagttgccaa agaatgccca tcgaaagtga ctgtcagctc atcaccgctt
    27301 tgcaacgggc cgacgccatc aggcgtgcct gtcagcacaa cgtcaccggc cttgagggta
    27361 aaaaacttgc tcatataagc gatcagcgga acaattttat ggatcatgtc cgcagtcgta
    27421 ccttgctgac gttgctcgcc gtttacgctc aggcccagcg ttgtattttg cgggtcgccg
    27481 gtaaattccg ccgcgggaat aaacccggaa agcggacaag agttatcaaa cgctttagcc
    27541 ttttcccacg gctgcccggc tttcttcatt tttccctgaa catcacgcaa tgtcagatcg
    27601 agcgccacgc cataaccggc aatggctttg cggacatgct cttccgtagc ctgacgcagc
    27661 gtcgcgccaa tcaacaccgc cagttcgact tcatgatgaa ctgaaccgaa atcggatggg
    27721 atcgccaatg gctgccgcag atcgcacagt gccgtttctg gtttaataaa cagcactggc
    27781 tcttcgggca ctgcgctgcc catctcttta atatgtttgg catagttact gccaacacag
    27841 actactttac tcaccggata atccagcagc gcaccttgcc agttgtgatg ttgatacata
    27901 acgttcccct caccttatcg tggatatgaa aacccgcgcc gacaaagata atgccaccgc
    27961 cggatatcgg ttatttgtta gtgtcgtctg ttttctgccc catgacggaa agatgttgct
    28021 tcagcaaatc ttcgggtggt ggcggtaact gcaaatagta accttgctcg gttaatgcca
    28081 gtttaacctt ttcaatatca gcattgacca gtttcttccg cccatccaac ggcagaatca
    28141 tcgctaactg aggctgacca aaacctttca tcagttcctc aggaacacgc gaaaaatcgt
    28201 cttttttttc gacatataaa taggtctggt cacgcttgct gcttcgataa atcacacaaa
    28261 acatactttt actctttaag atgccaggtt ttggcataag ctaacatcaa tgaaacccgc
    28321 gccacaagcc agccagctgt agatttctac ggtttgttga gtccatgccc ctttataagt
    28381 aatccccccg gcaaaaatgc cgcgccggga ttttaccgaa cgcaggaaag ccactggaat
    28441 tctcccgtta gggattgtta tattataaca gttcatcgta ctcattctga acaggagact
    28501 accaatgaaa atcaagagca tcagtaaggc tgtattgctg tttgcactgc taacctccac
    28561 gagctttgct gcgggtaaga atgtcaatgt tgagttcaga aaaggccata gcagcgctca
    28621 gtactccggc gaaataaagg gatacgatta cgatacatat accttctatg ccaaaaaagg
    28681 tcagaaagtg catgtgagta tctctaatga aggcgctgat acttacctct tcgggccagg
    28741 aatcgatgat tcagttgatt tatccagata ttccccagaa ctcgatagtc acggacaata
    28801 ctcactccct gcttctggca aatatgagct gcgagtgttg caaacccgaa acgacgcccg
    28861 taaaaacaaa actaaaaaat ataacgtcga tattcaaatt aagtaaatgt caaccgacgg
    28921 ggactctccc cgtcataaac gttaacgccc cactcctctc tgtcgctcaa gaggttaatg
    28981 acgaatagaa cataatgtaa acaagacatc actcacgaca cataaataac atctttgtgg
    29041 tggtttgatt gatttttcca ctccgcttac catctacacc aaaataacga tcatcacgac
    29101 ataatttttc tttaacatcg caaaatacac cgttagcgaa tgtaaattcg ctgggatcgt
    29161 agccttgtaa tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact
    29221 ctggggaaat gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg
    29281 gagattgaag tccgctagcg tcatgtccag acgccattga tgagaacatc atccccacac
    29341 ctgacaatcc gatataaata atacgcatca tattcatagc acatcctgat aatccaaccg
    29401 tacaacactt atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt
    29461 tacgatgacc tgacagaagt atcagacgtc ttctgcggtt tctatgatag agcaattctg
    29521 tggcggatat taagataacg ggtatctgtt ctttaatgat gccgcaaaaa atggaaagtt
    29581 aagaaagcct ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc
    29641 tggagatgct gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag
    29701 tacgacggca atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta
    29761 tcacagaata gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag
    29821 actataacat gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta
    29881 attgagtaag gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt
    29941 atctgtggtt catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa
    30001 aatcgctcag gcccccgcat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact
    30061 ggaagacccg gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt
    30121 tattggcgta agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct
    30181 gcctatcctg acggaaggaa aggaaaaagc gccacgtcca gctcccgcac cgcaggctcc
    30241 agcgcaaaat acaacgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg
    30301 tcagcgtatt tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc
    30361 cgaattgatt gccgatggta acattcatgt ctatggcatg atgcgcggtc gtgcgctggc
    30421 aggggcaagt ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaactggt
    30481 gtccatcgca ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc
    30541 ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt
    30601 taacaaggaa tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt
    30661 aagacaacct ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc
    30721 gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg tgaacgccgg
    30781 gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt
    30841 aaagataagc gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat
    30901 gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa
    30961 tttatcgttt gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt
    31021 gcagacgaag ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt
    31081 attttaggca ttctggcgtc gaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa
    31141 gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc
    31201 atggaagatg tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa
    31261 tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg
    31321 ggtaaagcct acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc
    31381 attgaagaag agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta
    31441 ctcgatttct ttctctcgcg gaagaaaaac acagccaaca ttgcaaaaga acggctgcag
    31501 attattgttg ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt
    31561 aaagatattc ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta
    31621 cagcttgagc aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa
    31681 gcagaagagc tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agagtcattt
    31741 ctgccttgag tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct
    31801 gatactcctc attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca
    31861 gaacaaaatg tttatgcctg gttgagtaaa taacctcatt gttagtgtgg atacactggg
    31921 ggatgataac cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg
    31981 taactggcgg gctttttttg ccccagaatt ttccctttta gccccctgta aacgaaaact
    32041 gcgccgaagc gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgca
    32101 ttccacgggg attccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac
    32161 gtactgctga atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca
    32221 ttgacaaaat taccgtcact attcactttc acccgattat catcgacaaa ttctttgctc
    32281 acagccgcct tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc
    32341 agcgttgcac ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca
    32401 ttggataaat gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg
    32461 caccagacca ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac
    32521 caaagacatc atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca
    32581 gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt
    32641 atctttcgac aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa
    32701 tatcttctgc tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt
    32761 acaacattgc gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt
    32821 tattcctcat ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc
    32881 acgctcattt tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt
    32941 gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa
    33001 aatacttgcc ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat
    33061 gaatcgcacc acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg
    33121 tgttaatggc gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa
    33181 aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagta ggtgccgccg ttggtgctgt
    33241 cactggcgtt ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg
    33301 tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt
    33361 actggcgggc ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa
    33421 cggtttgccg tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga
    33481 cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga
    33541 tttaaaaatt gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt
    33601 ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat
    33661 cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta
    33721 acggcgggat cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt
    33781 atcacccagc tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt
    33841 tacgccaagt ttaatttccc ccaaattact cgccccatct tgttcgactt tcacaccgtt
    33901 cattgagaca gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa
    33961 gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaaaccaag
    34021 tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttcggtgtg
    34081 cgcattatga tcgacgccca tccagatagc ctgtgcctgt ggctggaata tccacgtgta
    34141 tttcgccgct tccagaccaa aggatttatt cagggcaaag ccgtaacccg cttccagcga
    34201 gacggttgcg cctttcgcac tataggattc ataggctaat tcatcccctt tcaccgagtt
    34261 tttaaaccag ctatattgcg cccaggcgtc aatgtatgcg ccttttttac tgatgtcatc
    34321 ggcaaaccaa gtggcataca gccctgcgca atagcctctg acactccctt tcgaacgata
    34381 atccgacacg ctggaatgag ttgagttgta gtcgcgggca taaccagcca tcactcccaa
    34441 acgccaactg tcactgtcgg taaaaccacc ggttaacagc tcggccccta actgcgagac
    34501 gtagcgatgc gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc
    34561 aatttgacgt agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg
    34621 catttcaccc gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt
    34681 ggcgacgtaa cttccagctt cgaggcgcag gacgggggta ggcgcaacag taggcgctga
    34741 acttcctccg gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt
    34801 tttattggtc gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat
    34861 cctcgaatcc ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc
    34921 atttaacgtg taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga
    34981 ggtatttcca ttaataacga ctttatccgt agctgaatta tcatcaccca attcagttct
    35041 gagatgcaat acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc
    35101 gacatcccgt ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct
    35161 caccgttgag tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg
    35221 agtgttaaca tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac
    35281 ggtcaaatga cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc
    35341 cgtaatggtc acgttactcg tctcattttt atggtagagg atttcatttg ctgaccctag
    35401 tgagctacca gataaaatat ctaccgttgc ttcctgacca tttatggaag actccagcac
    35461 aattccagac ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt
    35521 ataaatttta gcagtcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga
    35581 aactgtcccc tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac
    35641 atcggttaat gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt
    35701 gccattagcg tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat
    35761 atccagtttt gctatggcat tattttcatt acttcctgta tcggtagtac tttcttcatc
    35821 aagagaagat acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc
    35881 atgattaccc gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga
    35941 accgtcagag ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag
    36001 caaaccaatt gagtaatccc ccgttatgac aatcgtgttt ttaccctgca aaataacagc
    36061 accgccaaat tcacttacga ttgcgctagc tgttaatgct gtgcttagag gactattgtc
    36121 agaaggatat aaaccgcccg ttgttacaat gacgccattg ttaatggtta acttgctacc
    36181 ctctcctgca acggataccc gtaacttcca ttacccgttg ttataactag cgcattatca
    36241 atatctttta cttttccatc ttttattgcc acagcatgtt tatattcatc gtgctgaata
    36301 aactgttcag catatgcatt acccaccgcc agagcaataa gtcccaaact aaacccggag
    36361 agttttttca gtttcataga ttaaccatcc attcattgga ttaacaaaca ttcctggtca
    36421 aataacctat gcgttcagat taccaaaacg gagggtaaag acaaaa
//
LOCUS       DAZFDP010000029        35363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFDP010000029 DAZFDP010000000
VERSION     DAZFDP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHJ1G_001600"
     CDS             complement(<1..434)
                     /locus_tag="ACHJ1G_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4763473.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGG"
     gene            complement(637..1038)
                     /gene="nikR"
                     /locus_tag="ACHJ1G_001601"
     CDS             complement(637..1038)
                     /gene="nikR"
                     /locus_tag="ACHJ1G_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4763474.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1044..1850)
                     /gene="nikE"
                     /locus_tag="ACHJ1G_001602"
     CDS             complement(1044..1850)
                     /gene="nikE"
                     /locus_tag="ACHJ1G_001602"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4763475.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1847..2611)
                     /gene="nikD"
                     /locus_tag="ACHJ1G_001603"
     CDS             complement(1847..2611)
                     /gene="nikD"
                     /locus_tag="ACHJ1G_001603"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4763476.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2611..3444)
                     /gene="nikC"
                     /locus_tag="ACHJ1G_001604"
     CDS             complement(2611..3444)
                     /gene="nikC"
                     /locus_tag="ACHJ1G_001604"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4763477.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3441..4385)
                     /gene="nikB"
                     /locus_tag="ACHJ1G_001605"
     CDS             complement(3441..4385)
                     /gene="nikB"
                     /locus_tag="ACHJ1G_001605"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4763478.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4385..5959)
                     /gene="nikA"
                     /locus_tag="ACHJ1G_001606"
     CDS             complement(4385..5959)
                     /gene="nikA"
                     /locus_tag="ACHJ1G_001606"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4763479.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6070..6657)
                     /gene="acpT"
                     /locus_tag="ACHJ1G_001607"
     CDS             complement(6070..6657)
                     /gene="acpT"
                     /locus_tag="ACHJ1G_001607"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4763480.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6712..7761)
                     /locus_tag="ACHJ1G_001608"
     CDS             complement(6712..7761)
                     /locus_tag="ACHJ1G_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4763481.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7893..9110
                     /locus_tag="ACHJ1G_001609"
     CDS             7893..9110
                     /locus_tag="ACHJ1G_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763482.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9114..9671)
                     /gene="dcrB"
                     /locus_tag="ACHJ1G_001610"
     CDS             complement(9114..9671)
                     /gene="dcrB"
                     /locus_tag="ACHJ1G_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4763483.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9744..10409)
                     /locus_tag="ACHJ1G_001611"
     CDS             complement(9744..10409)
                     /locus_tag="ACHJ1G_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4763484.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10630..10875
                     /gene="tusA"
                     /locus_tag="ACHJ1G_001612"
     CDS             10630..10875
                     /gene="tusA"
                     /locus_tag="ACHJ1G_001612"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4763485.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10977..13175)
                     /gene="zntA"
                     /locus_tag="ACHJ1G_001613"
     CDS             complement(10977..13175)
                     /gene="zntA"
                     /locus_tag="ACHJ1G_001613"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4763486.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQRVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13249..13875)
                     /locus_tag="ACHJ1G_001614"
     CDS             complement(13249..13875)
                     /locus_tag="ACHJ1G_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4763487.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14016..14375
                     /locus_tag="ACHJ1G_001615"
     CDS             14016..14375
                     /locus_tag="ACHJ1G_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4763488.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14378..14647)
                     /locus_tag="ACHJ1G_001616"
     CDS             complement(14378..14647)
                     /locus_tag="ACHJ1G_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4763489.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14637..15233)
                     /gene="rsmD"
                     /locus_tag="ACHJ1G_001617"
     CDS             complement(14637..15233)
                     /gene="rsmD"
                     /locus_tag="ACHJ1G_001617"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4763490.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15383..16879
                     /gene="ftsY"
                     /locus_tag="ACHJ1G_001618"
     CDS             15383..16879
                     /gene="ftsY"
                     /locus_tag="ACHJ1G_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4763491.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16882..17550
                     /gene="ftsE"
                     /locus_tag="ACHJ1G_001619"
     CDS             16882..17550
                     /gene="ftsE"
                     /locus_tag="ACHJ1G_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4763492.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17543..18601
                     /gene="ftsX"
                     /locus_tag="ACHJ1G_001620"
     CDS             17543..18601
                     /gene="ftsX"
                     /locus_tag="ACHJ1G_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4763493.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18846..19700
                     /gene="rpoH"
                     /locus_tag="ACHJ1G_001621"
     CDS             18846..19700
                     /gene="rpoH"
                     /locus_tag="ACHJ1G_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4763494.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19971..21074
                     /gene="livJ"
                     /locus_tag="ACHJ1G_001622"
     CDS             19971..21074
                     /gene="livJ"
                     /locus_tag="ACHJ1G_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4763495.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21273..21656)
                     /gene="panM"
                     /locus_tag="ACHJ1G_001623"
     CDS             complement(21273..21656)
                     /gene="panM"
                     /locus_tag="ACHJ1G_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4763496.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22080..23189
                     /gene="livK"
                     /locus_tag="ACHJ1G_001624"
     CDS             22080..23189
                     /gene="livK"
                     /locus_tag="ACHJ1G_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4763497.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23237..24163
                     /gene="livH"
                     /locus_tag="ACHJ1G_001625"
     CDS             23237..24163
                     /gene="livH"
                     /locus_tag="ACHJ1G_001625"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4763498.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24160..25437
                     /gene="livM"
                     /locus_tag="ACHJ1G_001626"
     CDS             24160..25437
                     /gene="livM"
                     /locus_tag="ACHJ1G_001626"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4763499.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25434..26201
                     /gene="livG"
                     /locus_tag="ACHJ1G_001627"
     CDS             25434..26201
                     /gene="livG"
                     /locus_tag="ACHJ1G_001627"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4763500.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26203..26916
                     /gene="livF"
                     /locus_tag="ACHJ1G_001628"
     CDS             26203..26916
                     /gene="livF"
                     /locus_tag="ACHJ1G_001628"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4763501.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27312..28628
                     /gene="ugpB"
                     /locus_tag="ACHJ1G_001629"
     CDS             27312..28628
                     /gene="ugpB"
                     /locus_tag="ACHJ1G_001629"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4763502.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28726..29613
                     /gene="ugpA"
                     /locus_tag="ACHJ1G_001630"
     CDS             28726..29613
                     /gene="ugpA"
                     /locus_tag="ACHJ1G_001630"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4763503.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29610..30455
                     /gene="ugpE"
                     /locus_tag="ACHJ1G_001631"
     CDS             29610..30455
                     /gene="ugpE"
                     /locus_tag="ACHJ1G_001631"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4763504.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30457..31527
                     /gene="ugpC"
                     /locus_tag="ACHJ1G_001632"
     CDS             30457..31527
                     /gene="ugpC"
                     /locus_tag="ACHJ1G_001632"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4763505.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31524..32267
                     /gene="ugpQ"
                     /locus_tag="ACHJ1G_001633"
     CDS             31524..32267
                     /gene="ugpQ"
                     /locus_tag="ACHJ1G_001633"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4763506.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32254..32694)
                     /locus_tag="ACHJ1G_001634"
     CDS             complement(32254..32694)
                     /locus_tag="ACHJ1G_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4763507.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32814..34556
                     /gene="ggt"
                     /locus_tag="ACHJ1G_001635"
     CDS             32814..34556
                     /gene="ggt"
                     /locus_tag="ACHJ1G_001635"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4763508.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34594..34878)
                     /locus_tag="ACHJ1G_001636"
     CDS             complement(34594..34878)
                     /locus_tag="ACHJ1G_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO4763509.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(35078..35233)
                     /gene="yrhD"
                     /locus_tag="ACHJ1G_001637"
     CDS             complement(35078..35233)
                     /gene="yrhD"
                     /locus_tag="ACHJ1G_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO4763510.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         8232 a         9160 c         9862 g         8109 t
ORIGIN      
        1 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
       61 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      121 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      181 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      241 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      301 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc aatacgtacc
      361 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcaccctg acgcgccgcc
      421 ggttttccgc tcatcgtaaa atccttttct attattttaa tgacagtatc ttcatacgaa
      481 aaagcgaaga aaaacttgtg gtcacaatga attgaagatt tttaggaata tatttagaac
      541 gttacaaaag agaaggttcg gatagggtat ttagagagga atcaggtgtg ctagtggccc
      601 tttgttgatc gttcaccaaa ggacccaata atagactcaa tcttccttcg gcaagcactg
      661 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
      721 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
      781 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
      841 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
      901 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
      961 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     1021 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     1081 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     1141 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     1201 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     1261 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     1321 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     1381 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     1441 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     1501 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     1561 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     1621 gccagcggtt cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc
     1681 aaccgcgcga gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg
     1741 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat
     1801 ccaccgtgcg catagtgatg ggaaaggccg gagatgttaa gtaaagtcat gatgccagct
     1861 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcactgta tgtttggggg
     1921 cgttaaacag cgtttctaca tcgccctgtt cgacaatctt accgtcagac atcaccgcca
     1981 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt caccagcagc attcccggcg
     2041 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     2101 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     2161 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     2221 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     2281 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     2341 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     2401 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     2461 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     2521 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     2581 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     2641 gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     2701 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     2761 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     2821 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     2881 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     2941 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     3001 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     3061 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     3121 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
     3181 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgt
     3241 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
     3301 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
     3361 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     3421 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     3481 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     3541 agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     3601 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg
     3661 atatgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt
     3721 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
     3781 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
     3841 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
     3901 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
     3961 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc
     4021 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
     4081 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
     4141 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc
     4201 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
     4261 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
     4321 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
     4381 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
     4441 aggggatgtt acccagctcc ggttttgata ccaccatcat tgagatgtaa ctgataggca
     4501 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
     4561 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggt ttgtcggcta
     4621 atccttgctg tgcctggaag tcagcgtgtg acggtacgcg cattgaactg aggaaggcgt
     4681 gtggatcata tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
     4741 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
     4801 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
     4861 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
     4921 tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg cgcggtttca
     4981 ggccgaggtt ggcgtagggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
     5041 catacaacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
     5101 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
     5161 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
     5221 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
     5281 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
     5341 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
     5401 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
     5461 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
     5521 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
     5581 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
     5641 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
     5701 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
     5761 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
     5821 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt
     5881 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
     5941 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
     6001 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
     6061 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
     6121 agtaggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
     6181 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
     6241 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
     6301 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agtgccagtt
     6361 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
     6421 ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcatttccg gcgcaaatgc
     6481 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
     6541 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
     6601 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
     6661 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
     6721 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
     6781 ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg
     6841 gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag
     6901 gcgatggccc atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa
     6961 caatgcgccg accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg
     7021 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac
     7081 tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc
     7141 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
     7201 gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac
     7261 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
     7321 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
     7381 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
     7441 tttaaaaagt ttgcgcgtca actccttatt aaacttcggc agcatagaga taaattcgtt
     7501 aaacgatgcc gccagtacgc cgaccagcgc agttagtgcg atcagcatca ccaccaccac
     7561 aatcgtaatg gcaatggggc gttgtactcc ccgacgaata aaccaggtga cgagcgggtt
     7621 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
     7681 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
     7741 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
     7801 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
     7861 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
     7921 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
     7981 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
     8041 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
     8101 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
     8161 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
     8221 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
     8281 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
     8341 gagtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
     8401 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
     8461 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
     8521 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggcg ctggcactgg
     8581 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
     8641 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
     8701 tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg
     8761 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
     8821 gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
     8881 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
     8941 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
     9001 cggtgattta tctggcggcg gcgggactgg tcacaatcgc gttattactg acgtggcgat
     9061 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
     9121 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct
     9181 ggcagagtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
     9241 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
     9301 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
     9361 tccagacgct tcgccagcac cgccagatct tcttttggat catcgcccat gatgacgatg
     9421 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
     9481 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
     9541 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
     9601 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
     9661 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
     9721 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
     9781 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
     9841 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
     9901 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
     9961 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactttgacg caggcggtta
    10021 aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca tgaaactggc ggtggcgata
    10081 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga acccatatag
    10141 aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc gaagataatg
    10201 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag aaaaataaac
    10261 ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg aagctgaacc
    10321 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    10381 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    10441 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    10501 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    10561 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    10621 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    10681 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa
    10741 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    10801 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    10861 cgtaaaggcg gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac
    10921 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    10981 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    11041 agcaccgcca gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    11101 agtcccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    11161 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcggc ggtttccagc
    11221 gccacgtctg tgccgctgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    11281 tcgttaatac catcaccgac catcgccagc ggcgcatgtt gattcagctt ggtcaccgct
    11341 ttgactttct cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    11401 gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaactcc cagcgcgttc
    11461 agttcactga tggcagttgc agcgtcggcg cgcagggtat cctgcaatgc aatgataccc
    11521 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    11581 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    11641 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga
    11701 atggcgagtt cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgca
    11761 ccttgctcga ccgccgctgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    11821 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    11881 tgggtaacac gacctagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    11941 gctgctgccg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg
    12001 cagccaatca gcagcagcgt caggccttta taaatccact cttgccagct ggcggcaaac
    12061 agcagcggtg gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata
    12121 cggctgaaac ggtcgataaa ccgctcaatg ggtgcgcgac gctcttcggc ttcttcgatc
    12181 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    12241 agacggtcta cgctggtggc tccagcaggg actttatcgc ccgttgcgcg ctccaccgga
    12301 atagattcgc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    12361 tcggcaggca aacgtccacc tgcggcgact tcaatcacat cgccagggcg caggctgtta
    12421 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    12481 attaacgcgc tgaccctttg acgtgcgcgg ctggcggccc agccttccag tcgttcgcca
    12541 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa aagcgcacca
    12601 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcaaccgt
    12661 aatgcctgac gagcaatcgg gtacagccca accagtgtgg tcgcgataaa cgccagttgc
    12721 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagcgta
    12781 atcagcggca gattctcttt caggcgtgat gcttgcggtt cgtcggcgga ctgttcatcg
    12841 cgcagggagt agcccgcttt ttgcaccgca gattcaacct gggcacggat gtcattgtcg
    12901 gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc
    12961 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    13021 cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg
    13081 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    13141 ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct
    13201 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    13261 cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    13321 acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga
    13381 agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    13441 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    13501 cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca ccagtggcag
    13561 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    13621 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    13681 gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt cgaacatcgg
    13741 cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata cccagcgttg
    13801 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    13861 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    13921 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    13981 atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    14041 ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa caacgacggg
    14101 aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg agcaacaagc
    14161 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    14221 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    14281 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    14341 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    14401 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    14461 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    14521 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt gggaagggat
    14581 gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa
    14641 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    14701 actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg
    14761 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    14821 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta
    14881 cctttttgcg ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    14941 agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtt
    15001 gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgcttcc ggcgaagcaa
    15061 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    15121 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    15181 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    15241 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    15301 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    15361 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    15421 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    15481 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga
    15541 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    15601 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    15661 gccggaaccg gtcgcagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    15721 gttgccagaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    15781 aaccgtagag attgtcgaag cggcagaaga agaagcggct aaagaagaaa tcaccgacga
    15841 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtagttcc
    15901 tccggcagaa gaagagcagc cggtggaaga aattgcccag gagcaagaaa aaccgaccaa
    15961 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    16021 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    16081 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    16141 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    16201 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    16261 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    16321 gctggcacgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    16381 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    16441 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    16501 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    16561 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    16621 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    16681 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    16741 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    16801 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    16861 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    16921 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    16981 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    17041 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    17101 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    17161 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    17221 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    17281 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    17341 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    17401 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    17461 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    17521 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    17581 tggcgggcgt cttgatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa
    17641 cgccccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgtaaaacca acgttttcaa
    17701 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    17761 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    17821 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    17881 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    17941 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    18001 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    18061 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    18121 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    18181 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    18241 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    18301 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    18361 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    18421 tctgatgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    18481 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    18541 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    18601 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    18661 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    18721 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    18781 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    18841 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    18901 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    18961 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    19021 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    19081 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    19141 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    19201 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    19261 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    19321 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    19381 acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    19441 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    19501 agcaggcggc aaaccgtctg accgacgcga tgcagggtct ggacgaacgc agccaggaca
    19561 tcatccgtgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    19621 gttacggtgt ttccgctgaa cgtgtgcgcc aactggaaaa gaacgcgatg aaaaaacttc
    19681 gcgccgctat tgaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    19741 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    19801 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    19861 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctga
    19921 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    19981 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    20041 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    20101 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    20161 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    20221 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    20281 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    20341 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    20401 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    20461 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    20521 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    20581 atttctctac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg
    20641 gttaccaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    20701 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    20761 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca
    20821 ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    20881 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    20941 ccaaatacct gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga
    21001 aaggcgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    21061 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    21121 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    21181 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    21241 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    21301 gccgtaaacc ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc caagcctgca
    21361 tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    21421 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    21481 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    21541 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    21601 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    21661 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    21721 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    21781 acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc
    21841 tttgtgccgt tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc
    21901 agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcacta ttatcacaaa
    21961 atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt
    22021 ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta
    22081 tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg
    22141 ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt
    22201 ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg
    22261 gaattaaggg cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag
    22321 ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt
    22381 gttcttcttc tacccagcct gcgtcagata tctatgaaga cgaaggtatt ctgatgatct
    22441 cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg
    22501 ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc
    22561 cccagcgcat cgccatcatt cacgacaaac aacaatatgg cgaagggctg gcacgttcgg
    22621 tgcaggacgg gctgaaagct gctaacgcca atgttgtttt cttcgacggc attaccgctg
    22681 gcgagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt
    22741 actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc
    22801 tgaaaaccca gtttatgggg ccggaaggtg tgggtaatgc gtcgctgtcg aatattgctg
    22861 gcgatgctgc cgaaggcatg ttggtcacca tgccaaaacg ctatgaccag gatccggcaa
    22921 accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct
    22981 ggatcactta cgcggcggtg caatctctgg caacagccct tgagcgtacc ggcagcgatg
    23041 agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc
    23101 tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg
    23161 ccgacggttc atccacggca gccaagtgat catcccaccg cccgtaaaat gcgggcgggt
    23221 ttagaaaggt taccttatgt ctgagcagtt tttgtatttc ttgcagcaga tgtttaacgg
    23281 cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat
    23341 tatcggcatg ataaacttcg cccacggcga ggtttatatg attggcagct acgtctcatt
    23401 tatgatcatc gccgcgctga tgatgatggg cattgatacc ggctggctgc tggtggcggc
    23461 gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtggc
    23521 ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggtatgtc
    23581 catcttcctg caaaactacg tcagcctgac cgaaggttcg cgtgacgtgg cgctgccgag
    23641 cctgtttaac ggtcagtggg tggtgggcca tagcgaaaac ttctctgcct ctattactac
    23701 catgcaggcg gtgatctgga ttgtcacctt cctcgccatg ctggcgctga cgattttcat
    23761 tcgctattcc cgtatggggc gcgcgtgtcg tgcctgcgcg gaagatctga aaatggcgag
    23821 tctgcttggc attaacaccg accgggtgat tgcgctgacc tttgtgattg gcgcggcgat
    23881 ggcggcggtg gcgggtgtgc tgctcggtca gttctacggc gtaattaacc cctacatcgg
    23941 ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc
    24001 aggggcgatg attggtgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct
    24061 gagtacggaa tataaggatg tggtttcatt tgccctgctg attctggtgc tgctggtgat
    24121 gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca
    24181 atggcgctgc tctctgccgc gatgtttttt gtcctggcgg gcgtctttat gggcgtgcaa
    24241 ctggagctgg atggcaccaa actggtggtc gacacggctt cagacatccg ttggcagtgg
    24301 gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgaccggc tttccagaaa
    24361 gggctaaaaa gcgtttccgg accgaagttt attctgcccg cgattgatgg ctccacggtg
    24421 aagcagaaac tattcctcgt ggcgctgctg gtgcttgcgg tggcgtggcc gtttatggtg
    24481 tcgcgtggga cggtggatat cgccaccctg accatgatct acattatcct cggcctcggg
    24541 ctgaacgtgg ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg cttttacgcc
    24601 atcggcgctt acacttttgc gctgctcaat cactattacg gcctgggctt ctggacctgt
    24661 ctgccgattg ccgggttaat ggcagcggcg gccggcttcc tgctcggctt cccggtgctt
    24721 cgcttgcgcg gcgactatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata
    24781 ttgctgctca ataacaccga aattaccggc ggcccgaacg gaatcagtca gatcccgaaa
    24841 ccgacactct tcggactcga gttcagccgt accgctcgtg aaggcggctg ggacacgttc
    24901 agtaatttct ttggcctgaa atacgatccc tccgatcgtg tcatcttcct ctacctggtg
    24961 gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg
    25021 gggcgtgcgt gggaagcgtt gcgtgaagac gagatcgcct gccgttcgct gggcttaagc
    25081 ccgcgtcgta tcaagctgac tgcctttacc ataagtgccg cgtttgccgg ttttgccgga
    25141 acgctgtttg ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg
    25201 gcgtttgtac tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg
    25261 gcggcaattt tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg
    25321 ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc
    25381 atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc
    25441 agccattatt atctgttaac ggtctgatga tgcgcttcgg cggcttgctg gcggtgaaca
    25501 acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg
    25561 gaaaaaccac ggtttttaac tgcctgaccg gattctacaa acccaccggc ggcactattt
    25621 tactgcgtga tcagcatctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg
    25681 tgcgtacctt ccagcatgtg cgtctgttcc gtgaaatgac ggtgattgaa aacctgctgg
    25741 tggcgcagca tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt
    25801 tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt
    25861 tgctggaaca cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg
    25921 agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag
    25981 gtcttaaccc aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc
    26041 acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc
    26101 gaatttatgt ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta
    26161 ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg
    26221 tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg
    26281 catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc
    26341 ttgctcggca cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat
    26401 aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg
    26461 gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt
    26521 tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gtttccacgt
    26581 ctgcatgagc gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg
    26641 gcgattggtc gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctc
    26701 ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag
    26761 gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc
    26821 ggctacgtgc tggaaaacgg ccatgtggtg ctctccgata ccggcgacgc gctgctggcg
    26881 aacgaagcag tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc
    26941 aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag
    27001 gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt
    27061 ttacgccgca tccggcatga aacgatgggt aatctgtaga gtttgattca gaccttctat
    27121 tttccgctta tccgtgcccc atctcccatt ttccctcacc cacgccgtca ccgccttgtc
    27181 atctttctga caccttacta tcttacaaat gtaacaaaaa agttattttt ctgtaattcg
    27241 agcatgtcat gttaccccgc gagcataaaa cgcgtgaatt cgcgcattcg gtacaacaag
    27301 agagataaac gatgaaaccg ttacattata cagcttcagc actggcgctc ggactggcgt
    27361 taatggggaa tgcacaggca gtgacgacca ttccgttctg gcattctatg gaaggggaac
    27421 tgggtaaaga ggtggattct ctggcccaac gttttaacgc cgaaaatccg gattacaaaa
    27481 ttgtaccgac ctataaaggc aactacgaac agaatttaag cgcggggatt gccgcatttc
    27541 gtaccggcaa cgctccggct attttgcagg tttatgaagt tggcaccgcc accatgatgg
    27601 catcgaaagc cattaaaccg gtatatgacg tgtttaaaga ggcggggatt caattcgatg
    27661 agtcgcagtt tgtgccgacg gtttccggtt actactccga cagcaaaacg ggccacttac
    27721 tctcccagcc attcaacagc tcgacccccg ttctctatta caacaaagac gccttcaaga
    27781 aagcaggatt agacccggaa caaccgccga aaacctggca ggatctggcg gactatgccg
    27841 cgaaactgaa agcctccggt atgaagtgcg gctacgccag cggctggcag ggctggatcc
    27901 aactggaaaa ctttagcgcc tggaacggtc tgccgtttgc cagcaaaaac aacggctttg
    27961 acggcacgga cgcggtgctg gagttcaaca agccggagca ggtgaaacat atcgccatgc
    28021 tcgaggagat gaacaagaag ggcgacttca gctacgtcgg tcgtaaggat gaatccaccg
    28081 agaagttcta taacggtgat tgcgcgatga ccaccgcctc ttccggttct cttgccaaca
    28141 ttcgcgagta cgccaaattt aattacggcg taggcatgat gccttacgac gccgatgcga
    28201 aagatgcgcc acaaaacgcc attatcggcg gagccagcct gtgggtgatg cagggtaaag
    28261 ataaagaaac gtataccggt gtggcgaagt tcctcgattt cctcgcgaag ccagaaaacg
    28321 ctgccgagtg gcatcagaaa accggttatc tgccaattac caaagcggcg tatgacctga
    28381 cccgtgagca gggcttttac gagaaaaacc caggtgcgga taccgcgacg cgtcagatgc
    28441 tgaataagcc gccgttgccg ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc
    28501 gcgtgattgt ggatgaagag ctggagagcg tgtggaccgg taagaagaca ccacagcagg
    28561 cactggatac cgccgttgag cgtggaaatc agttgctgcg ccgctttgag aaatcgacga
    28621 agtcttaatc agtgtaatgt cggatgcgtt tcgcttatct gacttggcat cgcgtgtagg
    28681 ccggataagc gaagcgcatc cggcacagtt caggaattaa ccgtaatgtc atcatcccgt
    28741 ccggtgttcc gctcgcgctg gctgccttat ctgctggtcg cgccgcagct catcatcacc
    28801 gttatctttt ttatctggcc tgcgggcgaa gcgttgtggt actcgctaca aagcgtcgat
    28861 ccgtttggtt tctccagcca gtttgtcggc ctggataact tcgtcgcgct gtttcatgac
    28921 agctactatc tcgactcctt ctggacgacg ataaaattca gcacctttgt caccgtcagc
    28981 ggtttgctgg tgtcgctgtt cttcgcggcg ctggtggagt acatcgtgcg cggcagccgt
    29041 ttctatcaaa ccttaatgct gctgccttac gccgtggctc ccgccgttgc cgccgtattg
    29101 tggatcttcc tgtttaaccc cggtcgcggg ctgatcaccc attttctcgc ggagttcggc
    29161 tacgactgga accacgcgca aaacagcggt caggcaatgt ttctggtggt gtttgcctca
    29221 gtatggaagc aaatcagcta caacttcctg ttcttctatg ccgcgctgca atccattccc
    29281 cgttcgttga tcgaagccgc agccatcgac ggtgcaggcc caattcgccg cttctttaag
    29341 attgcgctgc cgcttatcgc tccggtgagt ttcttcctgc tggtggtgaa cctggtgtat
    29401 gccttcttcg acaccttccc ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc
    29461 accacgacgc tgatttataa gatctaccgt gaaggcttta ccggactgga tctggcttcg
    29521 tctgccgcgc agtcggtggt gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc
    29581 cgctatgttg aaagcaaggt gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat
    29641 tcagccatac catgctgatc ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt
    29701 ttgtcgcggc gacgctggat aaacaggccg tctatgccgc gccgatgacg ctcatccccg
    29761 gcacacatct gctggaaaat atccacaaca tctgggttaa cggggtaggc acgaatagcg
    29821 cgccgttctg gcggatgttg cttaacagct ttgtgatggc gttcagcatt acgctcggca
    29881 aaattaccgt ctcgatgctc tcggcatttg ccattgtctg gtttcgtttt ccgctacgta
    29941 acctcttctt ctggatgatt tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc
    30001 cgacggtgga agtcatcgcc aacctgaaga tgctcgacag ctacgccggt ttaacgctgc
    30061 cgctgatggc ctcggcgacc gccacgtttc tgtttcgcca gttctttatg acgctgccgg
    30121 atgaactggt ggaagcggcg cggatcgacg gcgcgtcacc aatgcgtttc ttttgcgaca
    30181 tcgtttttcc gctctccaaa accaatctgg cggcgctgtt tgtgatcacc tttatttacg
    30241 gctggaacca gtatttgtgg ccgttgttga ttattaccga tgtggatctc ggcaccaccg
    30301 tggcagggat caaagggatg atcgctacag gcgaaggcac cactgaatgg aactcagtga
    30361 tggcggcgat gttgttaacg cttatccctc cggtggtgat tgttttagtg atgcagcgtg
    30421 ccttcgtgcg cggcctggtc gatagtgaga aataagatgg caggactgaa attacaggca
    30481 gtaaccaaaa gctgggatgg taaaacccag gtcattaaac cgctgaccct tgatgtggcg
    30541 gatggcgaat ttatcgtgat ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgc
    30601 atggttgccg ggctggagcg ggtgacagaa ggcgatatct ggatcaacga ccagcgcgtg
    30661 actgaaatgg agccaaaaga tcgcgggatt gcgatggtgt tccagaacta cgcgctttat
    30721 ccgcatatga gtgtcgaaga aaacatggcg tgggggctga aaattcgcgg catgggcaag
    30781 cagcaaattg ccgagcgcgt taaagaagcg gcgcgcattc tggagctgga cggtctgctc
    30841 aaacgtcgcc cgcgcgagct ttccggcggt cagcgccagc gtgtggcgat gggccgcgcg
    30901 attgtgcgcg atccggcggt gttcctgttt gatgagccgc tctctaacct cgatgccaag
    30961 ctgcgcgtgc agatgcgtct tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca
    31021 ctctacgtta ctcacgatca ggttgaagcg atgacgctcg cccagcgagt aatggtgatg
    31081 aacggcggcg ttgccgaaca gattggcaca ccagttgaag tctacgaaaa gcccgccagc
    31141 ctgtttgtag cgagttttat cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat
    31201 aacgaaggca cgcatttcga actggacggc ggtattgagc tgccgctaaa cggtggctac
    31261 cgtcagtatg ccgggcgtaa aatgactctc ggcattcgcc cggaacatat tgcgctaagc
    31321 tcgcaggcag aaggcggcgt accgctggtg atggacacgc tggagatcct cggcgcagat
    31381 aacctggcgc acggacgctg gggcgagcag aagctggtgg tgcgactggc gcatcaggag
    31441 cgcccgacgg caggcagcac gctgtggctg catctggcgg aaaatcagct gcatcttttt
    31501 gatggtgaaa caggacaacg agtatgagta actggcctta tccccgcatc gtcgctcatc
    31561 gtggcggcgg taagctggcc ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat
    31621 acggtcataa gatgatcgaa tttgacgcga agttatcgaa agatggcgag atcttcctgc
    31681 tccatgacga caatctcgaa cgcaccagca acggctgggg cgtcgcgggt gaactgaact
    31741 ggcaggattt actgcgcgtg gatgcgggca gttggtacag caaagcgttt aaaggtgagc
    31801 cgctgccgtt gctttcgcag gtggcggaac gctgtcgcga acacgggatg atggcgaata
    31861 tcgaaatcaa acccaccact ggcactggac cattaacggg caaaatggtg gcgctggcgg
    31921 cacgccaact gtgggccggt atgacgccgc cgctgctgtc atcgtttgag attgatgctt
    31981 tagaagctgc acagctggcg gcaccggaac tgccgcgtgg tttattgctt gatgagtggc
    32041 gcgacgactg gcgcgaactg accgcgcggc tgggctgcgt ctctattcat ctcaatcata
    32101 agttactcga taaagtgcga gtgatgcagt tgaaagacgc cggactgtgg attctggttt
    32161 ataccgtcaa taaaccccag cgcgcggcag agttgctgcg ctggggcgtg gattgcatct
    32221 gtaccgatgc gattgacgtg attggtccga actttacggc ccaatagttt tcaacggaat
    32281 gtcaggctgc ggcgtgctgg gctgattcag catgtcgccg tttctcctct ccggcagcat
    32341 atgctgctga ttcaacgaac tatccggatt acggttgctg tttaacatcc cgccgttggt
    32401 gttgggcagc atttgctgcc gggcgggatt tcgctccccc ggctgtgact gcaacacccg
    32461 ctgagaatta ttgtttatct ggttttctaa atgctgctgt tgcaactgag tttgcgtttt
    32521 cagttgctga ttcagcatcc ctttttgctg gatttgctga gtctgcatct gggtttgcat
    32581 ccgctgctgg ctgggtatct gataccccgg ctggttaggg ttgttcagag tattaatggg
    32641 ctgtgcaaag ccgacaaacg gcaggagtgc cgtaagaatc agaagccgtt tcatcgcgta
    32701 tcctcctctg aagatatcct ttaagtttac tcgcttcccg acaaaacgat gattaattca
    32761 gagttatata ccaggcttag ctggggttgc cccttaatct ctggagaata acgatgataa
    32821 aaccgacgtt tttacgccgg gtggccattg ctgctctgct ctcaggaagt tgttttagcg
    32881 ccgccgccgc gcctcctgcg ccgcccgtct cgtatggtgt ggaggaagat gtcttccacc
    32941 cggtacgcgc gaaacaggga atggtagcgt ctgtggacgc cactgccact caggtggggg
    33001 tggatattct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg ggctacgcgc
    33061 tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg ctgatccgct
    33121 cgaaaaatgg caataccacc gctatcgatt tccgcgaaat ggcacccgct aaagcgaccc
    33181 gcgatatgtt ccttgatgac cagggcaacc cggacagcaa aaaatcactc acttcgcatc
    33241 tggcttccgg cacaccaggt acggtcgctg gtttctcgct ggcgttggat aagtacggca
    33301 ctatgccgtt gaacaaagtg gtgcagcccg cgttcaaact ggcacgcgat ggttttatcg
    33361 ttaacgatgc tttggcagac gatctcaaaa cctacggtag cgaagtgttg ccgaatcacg
    33421 aaaacagcaa agctatcttc tggaaagagg gcgaaccgct gaaaaagggc gacacgctgg
    33481 tgcaggcgaa cctggcaaag agcctggaga tgattgctga aaacggcccg gacgaattct
    33541 ataaaggcac gattgcggaa cagatcgccc aggagatgca gaaaaacggt ggcttgatca
    33601 ctaaagaaga tttagcggcc tataaagcgg tcgaacgcac tccgataagc ggcgattatc
    33661 gcgggtatca ggtttactcc atgccaccgc catcctccgg cgggatccat atcgtacaaa
    33721 tcctcaatat tctggaaaac ttcgatatga agaaatacgg ctttggcagc gccgatgcga
    33781 tgcaaatcat ggcagaagcg gagaaatacg cctacgccga ccgctcggaa tatcttggcg
    33841 acccggattt tgtcaaagtc ccgtggcagg cactgaccaa taaagcctat gccaaatcca
    33901 ttgccgatca aattgatatc aacaaagcga agccgtccag tgagattcgc cctggcaagc
    33961 ttgcgcctta tgagagtaat caaactaccc attactctgt agtggataaa gacggtaacg
    34021 cggtggcggt gacctatacg ctgaacacca ccttcggtac gggtattgtc gcgggcgaga
    34081 gcggtattct gcttaataac cagatggatg atttctccgc caaaccgggc gtaccgaacg
    34141 tttacgggct ggtgggcggt gatgccaacg ccgtcgggcc gaacaaacgc ccgctgtcgt
    34201 cgatgtcgcc gaccattgtg gtgaaagacg gtaaaacctg gctggttacc ggtagcccag
    34261 gcggtagccg gatcatcact acagtgctgc aaatggtggt gaatagcatc gattatggca
    34321 tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg ccggacgagc
    34381 tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca aaaggtcaga
    34441 aagtggcgct gaaagaggcg atgggcagta cacaaagcat tatggttggg ccggacggtg
    34501 agttgtacgg cgcatccgac ccgcgctcgg tggatgattt aacggcgggg tactaaggtt
    34561 agcggccctc ttcgtgggaa gagggctatt ttgtcagggc aagccgaagg tagccttttt
    34621 tatttcgtaa tcctgtagat attcttccag cggctttgcc gttcccagag aatgaatttc
    34681 accactgtct ttatcaataa taaaaggtgc gttaccagct aagcgcgcgg cctcatctcc
    34741 agtttcgaga aattctcgtg cttcgaaaca gaaataccag ccctggctaa agcgtccatg
    34801 tagagtaatg acgaccggga gatctgcatc atcaaggtaa tggttcgctt tcgcgaatgc
    34861 gtcgtgataa gtaatcataa ttataataaa tattattgtt gagtgttata ttattattcc
    34921 tgtgtgatac attgagcaaa gacgcgttca tcttcgtcaa agatattatt aaatgctggt
    34981 tttgaaaaaa gttcatcgga taagatatag tcacgaaata agtatctgct aatattttat
    35041 gatttctttt tcagaacgtc gaacgggatt ttcttattta agatatcaga atcttatgat
    35101 tcgggtaaaa ttctaaataa atcaacatgt cattaaaatc atcatagcca ttagctattt
    35161 cggctaaaat agagactaca tgtcttcggt ccatctcact taaggagtgt agttccgttg
    35221 taagtttttc catagcttgc actgctaaat ttcgaacaag gaattttctg ctggtaatct
    35281 ctaaaaagat ggcatggttt acaatgattt ttgtttcctt ttgattatta tgaacaactg
    35341 tccatgattt cgtttaagaa tga
//
LOCUS       DAZFDP010000030        34456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFDP010000030 DAZFDP010000000
VERSION     DAZFDP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1456)
                     /locus_tag="ACHJ1G_001638"
     CDS             complement(<1..1456)
                     /locus_tag="ACHJ1G_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4763511.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
     gene            complement(1886..4300)
                     /gene="ybbP"
                     /locus_tag="ACHJ1G_001639"
     CDS             complement(1886..4300)
                     /gene="ybbP"
                     /locus_tag="ACHJ1G_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4763512.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(4297..4983)
                     /gene="ybbA"
                     /locus_tag="ACHJ1G_001640"
     CDS             complement(4297..4983)
                     /gene="ybbA"
                     /locus_tag="ACHJ1G_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4763513.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            4954..5577
                     /gene="tesA"
                     /locus_tag="ACHJ1G_001641"
     CDS             4954..5577
                     /gene="tesA"
                     /locus_tag="ACHJ1G_001641"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4763514.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5567..6376
                     /gene="ybbO"
                     /locus_tag="ACHJ1G_001642"
     CDS             5567..6376
                     /gene="ybbO"
                     /locus_tag="ACHJ1G_001642"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4763515.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            6437..7291
                     /gene="cnoX"
                     /locus_tag="ACHJ1G_001643"
     CDS             6437..7291
                     /gene="cnoX"
                     /locus_tag="ACHJ1G_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4763516.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(7354..8133)
                     /gene="fetB"
                     /locus_tag="ACHJ1G_001644"
     CDS             complement(7354..8133)
                     /gene="fetB"
                     /locus_tag="ACHJ1G_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4763517.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(8120..8797)
                     /gene="fetA"
                     /locus_tag="ACHJ1G_001645"
     CDS             complement(8120..8797)
                     /gene="fetA"
                     /locus_tag="ACHJ1G_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4763518.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            8943..9860
                     /gene="qmcA"
                     /locus_tag="ACHJ1G_001646"
     CDS             8943..9860
                     /gene="qmcA"
                     /locus_tag="ACHJ1G_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4763519.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9857..10315
                     /gene="ybbJ"
                     /locus_tag="ACHJ1G_001647"
     CDS             9857..10315
                     /gene="ybbJ"
                     /locus_tag="ACHJ1G_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4763520.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10316..10723)
                     /gene="cueR"
                     /locus_tag="ACHJ1G_001648"
     CDS             complement(10316..10723)
                     /gene="cueR"
                     /locus_tag="ACHJ1G_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4763521.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10720..10817)
                     /locus_tag="ACHJ1G_001649"
                     /pseudo
     CDS             complement(10720..10817)
                     /locus_tag="ACHJ1G_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10848..12140)
                     /gene="ybaT"
                     /locus_tag="ACHJ1G_001650"
     CDS             complement(10848..12140)
                     /gene="ybaT"
                     /locus_tag="ACHJ1G_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4763522.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(12143..13075)
                     /gene="glsA"
                     /locus_tag="ACHJ1G_001651"
     CDS             complement(12143..13075)
                     /gene="glsA"
                     /locus_tag="ACHJ1G_001651"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4763523.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            13337..15841
                     /gene="copA"
                     /locus_tag="ACHJ1G_001652"
     CDS             13337..15841
                     /gene="copA"
                     /locus_tag="ACHJ1G_001652"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4763524.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(15955..16296)
                     /gene="ybaQ"
                     /locus_tag="ACHJ1G_001653"
     CDS             complement(15955..16296)
                     /gene="ybaQ"
                     /locus_tag="ACHJ1G_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4763525.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            16434..17228
                     /gene="ybaP"
                     /locus_tag="ACHJ1G_001654"
     CDS             16434..17228
                     /gene="ybaP"
                     /locus_tag="ACHJ1G_001654"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4763526.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            17432..17911
                     /gene="ybaK"
                     /locus_tag="ACHJ1G_001655"
     CDS             17432..17911
                     /gene="ybaK"
                     /locus_tag="ACHJ1G_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4763527.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17948..19600)
                     /gene="ushA"
                     /locus_tag="ACHJ1G_001656"
     CDS             complement(17948..19600)
                     /gene="ushA"
                     /locus_tag="ACHJ1G_001656"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4763528.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            19818..21038
                     /gene="fsr"
                     /locus_tag="ACHJ1G_001657"
     CDS             19818..21038
                     /gene="fsr"
                     /locus_tag="ACHJ1G_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4763529.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            21276..22952
                     /gene="ybaL"
                     /locus_tag="ACHJ1G_001658"
     CDS             21276..22952
                     /gene="ybaL"
                     /locus_tag="ACHJ1G_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4763530.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(23085..24389)
                     /gene="gsk"
                     /locus_tag="ACHJ1G_001659"
     CDS             complement(23085..24389)
                     /gene="gsk"
                     /locus_tag="ACHJ1G_001659"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4763531.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            24541..25500
                     /gene="aes"
                     /locus_tag="ACHJ1G_001660"
     CDS             24541..25500
                     /gene="aes"
                     /locus_tag="ACHJ1G_001660"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4763532.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(25497..26459)
                     /gene="hemH"
                     /locus_tag="ACHJ1G_001661"
     CDS             complement(25497..26459)
                     /gene="hemH"
                     /locus_tag="ACHJ1G_001661"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4763533.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(26695..27339)
                     /gene="adk"
                     /locus_tag="ACHJ1G_001662"
     CDS             complement(26695..27339)
                     /gene="adk"
                     /locus_tag="ACHJ1G_001662"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4763534.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(27520..29394)
                     /gene="htpG"
                     /locus_tag="ACHJ1G_001663"
     CDS             complement(27520..29394)
                     /gene="htpG"
                     /locus_tag="ACHJ1G_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4763535.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(29504..30109)
                     /gene="recR"
                     /locus_tag="ACHJ1G_001664"
     CDS             complement(29504..30109)
                     /gene="recR"
                     /locus_tag="ACHJ1G_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4763536.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(30109..30438)
                     /gene="ybaB"
                     /locus_tag="ACHJ1G_001665"
     CDS             complement(30109..30438)
                     /gene="ybaB"
                     /locus_tag="ACHJ1G_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4763537.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(30491..32422)
                     /gene="dnaX"
                     /locus_tag="ACHJ1G_001666"
     CDS             complement(30491..32422)
                     /gene="dnaX"
                     /locus_tag="ACHJ1G_001666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4763538.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(31094..31158)
                     /gene="dnaX"
                     /locus_tag="ACHJ1G_001666"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(32551..33102)
                     /gene="apt"
                     /locus_tag="ACHJ1G_001667"
     CDS             complement(32551..33102)
                     /gene="apt"
                     /locus_tag="ACHJ1G_001667"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4763539.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(33255..33632)
                     /gene="ybaN"
                     /locus_tag="ACHJ1G_001668"
     CDS             complement(33255..33632)
                     /gene="ybaN"
                     /locus_tag="ACHJ1G_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4763540.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            33702..34229
                     /gene="priC"
                     /locus_tag="ACHJ1G_001669"
     CDS             33702..34229
                     /gene="priC"
                     /locus_tag="ACHJ1G_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4763541.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            34243..34404
                     /gene="rsmS"
                     /locus_tag="ACHJ1G_001670"
     CDS             34243..34404
                     /gene="rsmS"
                     /locus_tag="ACHJ1G_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4763542.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
BASE COUNT         7865 a         9359 c         9087 g         8145 t
ORIGIN      
        1 acaccaccgc cgtcagctgg ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg
       61 gtgtggtgat gtccgtgata tcgccggaca ccacattcag accgtactct gtccgccgtc
      121 ccgccgcgtc cgtctgcgcc gtgagccttc ctgccgcgtc atacccgctg tgcgtgacgc
      181 tgccgtccgc cagttctttt ttcaccaccc gcttcagccc ggccccgcct tctgtatgca
      241 gcacctcacg ccggttcagg ctgtccgtga cggtgatgcg gtcctgctca tactggtagc
      301 ggtaactcag gcctgccggg ttaagttgct ccaccacccg ccccgcatcg tcgtagcggt
      361 agcgcatctc cggcctgcct gcataacggt gccccaccat ccggccccgg tatttatcat
      421 cgtaagtaaa gtggcgcatc tgcgtaccgc tgcggtcata caccgccgcc agctcaccgc
      481 gtggcgtcca gtcgtagcac accagtggcg cggcaggtaa attttccggg tattccgggt
      541 cgtgcatcag ccacaccgcc gaaagacgga taccgctgtc agcgccgtat tccgtaccgg
      601 gcagtgtgtc ggggaaggaa ggagctgata gagggcgggg actgtcaggg gaagataacg
      661 aagcggtgtg cggtttacgg gcctcttccg cccgctgtgc ctgtgtggtc agtaccagac
      721 ggaactcccg cccggcaccg tccgtcacgc cggtgatttc cccggccagg tcaccggcgg
      781 cctcacgccg gtacgccagc gtccgcccga agcggtccgc catcccggta agcacccggt
      841 acggcggcag cggcgctggc agtaagtcct cagcacccgg cacccgctca gaccacccca
      901 gtatccacca cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga
      961 tatccggtgg cagcgacgcc cacagccgtg ccagcgtgtg gccgtccggc tgcgctgcct
     1021 taccgccacg caccagccac attgactcac tgcggctgta caccgcctcc cccggcagca
     1081 gcggctcaaa gtgaatgctc cgcccgccgt tgtcgttgag tatcagtgca tcatcgcgta
     1141 tctgtaagcg gatatcagaa ggcgctttcc agccggggcc gaaaacgccc accggtgcag
     1201 gcgtcttcgt ccggtagctg ctgtaggtgc gggagagaat gaacggcagc gggccgggca
     1261 gcgcaaggtc cgtctcgccg ggctgtactt ttgcaccgag cagcgggttc accgggttgc
     1321 ccgaggtcat cccgcccgga cacaccgagc acgccacgcc agttggcgcg ccaattctta
     1381 cacctgccga accctggaca atgggaccgc catactgagt catatctccc tgacgcgccg
     1441 ctggttttcc gctcatataa agtgcccttt tagttattgt tcatatccct gtgttagcaa
     1501 caaaaaaaaa taaaatgaca cacaagacca gctaatgtat ttatatattt ttcacataaa
     1561 cttatttgtt ctcaatcaac aacaactgtt attacaggtc actttcaata ttttattaaa
     1621 ctaaaatttt tagcacagat gaattcatca tcaacaaatt cactatatgg ataatatcca
     1681 ttcaacgcat atgatttatg attttattag tctgagttat ttttgtaggg ctatatttaa
     1741 aattattaaa ctccaccgat tcagattaat atcgcaatag aaagatagga aatcaccaat
     1801 tcaataacat catcaatgaa acatattgta gaattaattt aaacaaggcg ttccattcaa
     1861 ccagaacgcc ccttatttac tctcatcacc ccgcaaactg cctgaacagc gccttaccct
     1921 taaccagtcg cgcacccagc cagccgccga aaagcgacag cagcagcgca ccgctgcacg
     1981 gcagaacaat ccacaatcgc cagtctggct cccacgggaa atcaaacact ttcgcctgca
     2041 acactgccag tgccgtttcc gcaccaattg cggccaccag gccggaaaca aacccaagca
     2101 tggcgaactc acaccacaat gtcgtgcgca gcagtttttt ccccgcaccg agtgtgcgcc
     2161 ataccaccag ctcctgatga cgctggcgca ttcccacctg cacctgtgcc agcaacagca
     2221 acataccgca ggcggtgacc agcaccacca tcacctccag cgcccgactt acctgctcca
     2281 gcacctgacc gacctgtttt aaaatcgcgc caatatctaa caggctaatg gtcgggaact
     2341 ggcggttgag ttgtgtcaac atgccgttgc cattctccca gcggaaactg gtaagccagc
     2401 tctgcggttg cccgtctaat gccccttcag ggaaaataaa atagaaatta ggccgcagac
     2461 tttcccagtc cactttgcgc aggctggtca ctttagcgcg gaactcctgg gtatcgccca
     2521 taaaagtcac ggtatcaccg agggcaacgt ttaagcgttt cgccagcccc tcttccatcg
     2581 acacttcatc ggcttttggc ggccagttac cggcgacaat cggattatga tcgggtcgcg
     2641 tattttgcca ggtaagattg agttcgcggt taagcgcctc atcttcatta ccttctgtcg
     2701 gcttatcgtt aatcgccgtc agccgcgccc gcactaccgg ataaaatgat tccgggacta
     2761 tctgatgttc cgcgaggaac gcttttagcg gttctacctg ttctgtggca atgttaatta
     2821 aaaagtagtt cgggctttct ggaggtagct gctgctgcca gcggtcaagc agatcgccac
     2881 gcaataccag caacagcgcc agcagcataa aggagagcga aaatgccgaa agctggctta
     2941 atgttgacca cggctgacgt aacaggcggc taaccgccag gcgcagaggt agcgatttca
     3001 gcgtcatgcg gcgaagtaca ttcagcagca tccagcccag cacaccgcac agcaaagcca
     3061 gtactaccgc gcccgccagc accgcccaaa gcagcatgct gccacccatt aatccggcga
     3121 gcagcagcac aaccaccaca ctgacaatcg gcagataaaa cttcagcggc cagacgttcg
     3181 ctaccacatc attacgtaat acgcgtaaag gctgcgttgc cagcaacagg cggtacggtc
     3241 gtagccccac cagcagcgag atgaccgtca tggtacccag cgcccacagc cacggccaga
     3301 ggctggctgg cggtagtgca gcaggcagaa cgggcttgag cagcaccatc aacacgtttt
     3361 cgaacaacag gcctatcgcc ccaccggtaa cggctgaaag cgccagcacc atcaaccact
     3421 gaccgacgat tagcttacgc agttgcgctc gccctgcccc cagcgttttg aggatcgcca
     3481 ccagatcgta gcgactgcga cagtaatgat tcatcgctac cgccaccgct gccactgcca
     3541 gcagcaaggt cagaagcgcc gaaagcagca ggaactgttg cgagcgttcc atcgatcgcc
     3601 ccagcgcgcc ttcgtcctgt tccagaccgt accagcgttg ttcgggttta agctgaggta
     3661 acaaccattt ctcatagccg tcgagctggt tctcgttgcc gccgaattta taacgccagg
     3721 tgacccgact ccccggctgc acggctccgg ttttatcgac atccgccaga ttcatcatca
     3781 gacgcggagc catctggaag gggttaaaac cggaatccgg ttcctgaatc acttctccgg
     3841 caatccgcaa ggtggcatcg cccacgtcaa tggtgtcgcc cgttttcagg ttaagcagtg
     3901 ccatcaggcg tggggccagc aatacgctgc ccgcctgcgg tttcaggcca gggggattag
     3961 tttgcagatc gccatacatc gggtagatat catccaccgc tttgacgttc gccagctgcg
     4021 gtgtgtcgcc tgcaaatgtc atggtggcga aagtcagctg cttgccgact ttcaggccgc
     4081 gcttttgcgc ttcctccagc catgcttgcg gcacttcgcg cgaactgcgc aacgcccgat
     4141 cgcccgccat aaactcacgg ctttgctggc ttaagccctt ctccatgcga tcgctgatat
     4201 tgcccagcgc cagcacgcag gccaccgcca ggcttagcgc cagccagaca attaatagcg
     4261 acggcgagcg ccattcgcgc cagaaccaac gtgcaatcat gcttcctcct gcaactgccc
     4321 gttcaccagc cgtaagcagc ggtcgcagcg tgccgccagt tgcaggtcgt gagtcaccat
     4381 aatcaacgtg gtgccatgtt cacggttgag ggaaaacagc aggtcggcaa ttttatcgcc
     4441 cgtctggcgg tcaaggttgc cggtgggttc gtcggcaaac agcacatcag gtcgaccatt
     4501 aaaggctcgt gccagcgcca ctcgttgctg ttcaccgccg gaaagctgtg ccggaagatg
     4561 atccagacgt ttacccagcc ctaactgttc gagcaacgct ttcgccccgt tacgactttc
     4621 cgcgctactc tcaccgcgca gcagagccgg aagctcgaca ttttccagcg cgttaagggt
     4681 aggaattaac ataaatgact gaaaaacaaa gccgacgtgc ttcgcgcgca actttgcccg
     4741 cgcttcttcg tccatattat gtagcggttg tcccaccaga ctcacttcgc cactgctgcc
     4801 gtcatcaagc ccggcgagga tcgccagcaa ggttgactta cccgatcccg attcgcccac
     4861 cagtgcgatg gtctcgccac gtttgacaac cagctcaact ccggtgagga tggagagttc
     4921 atgctccccc tgaccgacgg acttcttaag atgatgaact tcaacaatgt tttccgctgg
     4981 catttgccct tcctgttcct ggtcctgtta accttccgtg ccgccgcagc ggacacgtta
     5041 ttgattctgg gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc ggcctggcct
     5101 gccttgttga atgataagtg gcagagtaaa acgtcggtag ttaatgccag catcagcggc
     5161 gacacctcgc aacaaggact ggcgcgcctt ccggctctgc tgaaacagca tcagccgcgc
     5221 tgggtgctgg ttgaactggg cggcaatgac ggtttgcgtg gttttcagcc acagcaaacc
     5281 gagcaaacgc tgcgccagat tttgcaggat gtcaaagccg ccaacgctga accattgtta
     5341 atgcaaatac gtctgcctgc aaactatggt cgccgttata atgaagcctt tagcgccatt
     5401 taccccaaac tcgccaaaga gtttgatgtt ccgctgctgc ccttttttat ggaagaggtc
     5461 tacctcaagc cacaatggat gcaggatgac ggtattcatc ccaaccgcga cgcccagccg
     5521 tttattgccg actggatggc gaagcagttg cagcctttag taaatcatga ctcataaagc
     5581 aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg gatgttccag
     5641 tggaattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg tgctggcagg
     5701 ttgccgaaaa ccggatgatg ttgagcgtat gaacaacatg ggatttaccg gcgtgttgat
     5761 cgatctggat tcaccagaaa gtgttgatcg cgcagcagac gaggtgatcg ccctgaccga
     5821 taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc ccctttccac
     5881 catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg cacaccagct
     5941 caccatgcgc ctgttacccg cgatgttacc gcacggtgaa gggcgtattg tgatgacatc
     6001 atcggtgatg ggattaatct ccacgccggg tcgtggcgct tacgcggcca gtaaatatgc
     6061 gctggaggcg tggtcagacg cactgcgcat ggagctgcgc cacagcggaa ttaaagtcag
     6121 cctgatcgaa cccggtccca ttcgtactcg cttcaccgac aacgtcaacc agacgcaaag
     6181 tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt acgttgggac cggaagcggt
     6241 ggtggacaaa gtacgccatg cttttattag cgagaagccg aagatgcgct atccggtaac
     6301 gctggtgacc tgggcagtaa tggtgctcaa gcgcctgctg ccggggcgcg tgatggacaa
     6361 aatattgcag gggtgagttg aagcgcgcgc ttaagccccc atgtcaaaga aaatatcgac
     6421 aacagagagc gactccatgt ccgtagaaaa tattgtcaac attaacgaat ctaacctgca
     6481 acaggttctt gaacagtcga tgaccactcc ggtgctgttc tatttttggt ctgaacgtag
     6541 ccagcactgt ttgcagttaa ccccgatcct ggaaagcctc gcggcgcagt acaacgggca
     6601 atttattctg gcgaagctgg actgcgacgc ggagcagatg attgccgccc agtttggtct
     6661 gcgcgcgatt ccgaccgtgt atctgttcca gaacgggcaa ccggtagatg gcttccaggg
     6721 gccgcaaccg gaagaggcga tccgcgccct gctggataaa gtgctgccgc gcgaagaaga
     6781 gctgaaagcg cagcaggcga tgcaactgat gcaggaaggc aattacaccg atgccctgcc
     6841 attgctgaaa gacgcctggc agttgtcgaa tcagaacggg gagatcggcc tgctgctggc
     6901 agaaacgctg attgcgctga accgttctga agatgcggaa acggtgctga aaaccattcc
     6961 gttgcaggat caggacaccc gctaccaggg gctggtggcg caaatcgaac tgctgaagca
     7021 ggcggctgat acgccggaaa ttcaacagtt gcaacagcag gtggcggaga atccagaaga
     7081 tgccgcactg gcgacgcaac tggctctgca actgcaccag gttgggcgca atgaagaggc
     7141 gctggagttg ctgttcgggc atctgcgtaa agatctcacc gccgcagacg gtcagacgcg
     7201 taaaacgttt caggagatcc tcgctgcgct gggtacgggt gatgcactgg cgtcgaagta
     7261 tcgccgccag ctgtatgcat tgttgtattg atattccgtt gatcggcaca tgaaccacaa
     7321 cgccggatgc gatgctgcgc atccggcatc gcatcatttc ttcttcaatt gcgtcaccac
     7381 caactggtgg cgcgaattat aaaacttacg ataggttaaa tagcaggcaa taatggtcga
     7441 caagctggcg gttgagagca gcataaaggt caccataatc tgatatttaa tcgccttcac
     7501 cggatcaatc ccggcaaata tcagcccgga catcattcca ggtaaactca ctaagccaac
     7561 cgtttttgct gaatcgaccg tcggaattaa agccgcgcga atactgtcgc gaatcaatat
     7621 cgctgaagcc tgcttcggcg tcgcaccaag actcagtttc tcctgaatct gttgctgttc
     7681 gctaatgacc cgttgcccta aattgttgta acacaacccc accgctacca tggcgttacc
     7741 ggcaatcatc ccggcgatag ggatcacctg catcgggata aattcaatcg acccagagag
     7801 aatcagcacc gccagggtaa ttcccgcccc gaccgtaatg gcaataaacg atgagataaa
     7861 agctttagca atatatttac tgcgtttttg cgcgttccac gccgcattaa agcagataaa
     7921 taacaccatc aataatgtca ggctggcatc atccacgctg aaaatatact tcagcacata
     7981 gccgacaata atcagctgaa ttatcgctcg cccgacgctc cagaaaatat ctttctccag
     8041 cgccagtttt tctttatggc taattaagat tgccaccacc accagcatta atgccagtgc
     8101 caatgattcg ttagtaatat tatgcgagtt catagcgtgc ttcctgcatt tctccggcat
     8161 gcggttgcag tgtaatcact ttatccgcat gattaatttc gtctttatcg tgtgtcaccc
     8221 acagcacggc aatattttgc tcgcgcacat aacgatggat catctcatta acgttatgtt
     8281 tattactttc atccagcgca ctggtgattt catccagcaa taaaaccttc ggcataaatt
     8341 gcaggttacg aatcagtgag atgcgttgtt tttcaccacc agatagctcg gcgatatttt
     8401 tcgtcaaaat gctgtccggc aaagcgaagc gttcgagaaa atcgagaaaa atggccgggt
     8461 caggctgctg gttacggatc tgccagggaa agatcagatt atcgtatacc gtatcgccaa
     8521 acagcgtcgg cgtctgggcg cagtaagaga cttgctggcg gtagatttct ggctttagtg
     8581 tgctgacatc ctcaccttca aacagtaacg ttccgttggt tgggctgatc aatgaagcaa
     8641 ctatttttag cagcgtactt ttgccacaac cggaaggacc ggtaattaac ttaaattcgc
     8701 cagcacgcag cgaaaaattg atgttattaa gaatcttcgc atcacccgcc agatatccta
     8761 cgttttgtag ctgaagtaaa ggactatttt cctgcatcac tgttcccttt ttctgatttt
     8821 tactaaaaac agtttatcct tcgcaggaat aagggggaac tctctttcag taatcaggta
     8881 taatttgttt aatcaagcag caattttgct gattatggac ggtacaacag gaggtttttc
     8941 cgatgcttat ctttatcccg attctcattt ttgtcgcgct ggtcattgtc ggcgcgggcg
     9001 tcaaaatcgt gccgcagggc tatcaatgga cggtagaacg ttttggtcgc tataccaaaa
     9061 cgttacagcc ggggctcagt ctggtggtgc cgtttatgga tcgcattggt cgcaagatca
     9121 atatgatgga gcaagtgctc gatatccctt cccaggaagt tatctcgaaa gataacgcca
     9181 acgttaccat cgacgcagtc tgttttattc aggtgattga cgcgccgcgc gcggcttatg
     9241 aagtcagcaa tctggagctg gcgatcatca acctgaccat gactaacatc cgtaccgtgt
     9301 tgggttcaat ggaacttgac gaaatgctct ctcagcgcga cagcatcaac tcacgcctgc
     9361 tgcgtattgt cgatgaagcc accaacccgt gggggattaa agtcacccgt attgaaattc
     9421 gcgacgtgcg cccaccggca gagcttatct cttcaatgaa cgcgcagatg aaagcggaac
     9481 gtaccaaacg cgcttacatt cttgaagcgg aagggatccg tcaggcggaa atcctcaaag
     9541 ccgaaggtga aaaacagtcg caaatcctga aagcggaagg cgaacgtcag tcggcgtttt
     9601 tacaggctga agcgcgtgaa cgttccgccg aagcagaagc ccgcgccacc aaaatggtgt
     9661 ctgaagctat cgcctccggt gatattcagg cggtgaacta cttcgtagcg cagaaataca
     9721 ccgaagcgtt acagcagatc ggttcctcca gtaacagcaa agtagtgatg atgccattag
     9781 aggccagcag cctgatgggg tcgattgccg ggattgccga gctggtgaaa gacagcgcca
     9841 acaaacggac tcagccatga tggagttaat ggtcgttcat ccacatattt tctggctcag
     9901 tctcggcggt ttgctgctgg cagccgagat gctgggcgga aatggttatt tgttgtggag
     9961 cggcgtggca gcggtgatta ccggcctggt ggtctggctg gtgccgctgg gttgggagtg
    10021 gcaaggggta atgtttgcca tcctgacgct gctcgccgcc tggctgtggt ggaaatggtt
    10081 gtcgcggcgg gtgcgcgaac aaaagcacag cgatagtcat ttaaaccagc gcgggcagca
    10141 gctgattggc cgacgttttg tgctggaatc tccgctggtc aacgggcgcg gtcatatgcg
    10201 cgtcggtgac agttcatggc ctgtcagcgc cagcgaggat ctcggtgcag gtacgcatgt
    10261 tgaagtcatt gcgatagaag ggataaccct acatatccga gccgtctcgt cttaatcacc
    10321 ctgcccgatg atgacagcag ccggagagat tttcgataat cgggcagtcg gcgctgtcat
    10381 cgccagggca ggcattcgcc agtgccagca gctggtcgcg catggattgc agctcctcaa
    10441 tgtgtcgttc gatctccgcc accttctcca gcgtgcgccg tttgacgtcg gcgctgtgcc
    10501 gctgcgggtc gttaaacaga ttcaccagct cgccgctctc ttccaggtta aagcccacct
    10561 gccgtgcctg gcgcagtaag gtcagttcgt tgagatgctg ctgcgtgtag gtgcgataac
    10621 cgttttcgct gcgcatcggc ggcgtcacca gccccttctc ttcatagaag cgaatggctt
    10681 tgctggtcag gccggtaatt tttgctacat cgctgatgtt catcgttcgc gcaacgcctc
    10741 ctgtgctttg ttaaagggtt tcaacagata ttcaagcacg tttttttgcc agtgcgaata
    10801 tcgaccgcca ccacaataat acaggaagta ctgcgcacag agagatgtta tacggtttta
    10861 ttgcgcttca tgaccattgc cacaataagg ctgagtaaga gtgagccaat aatccatatc
    10921 aacgcacggc taccctggga ccacagactg tagataaagc caacgatcac caacaacatc
    10981 accaacgtac caacgataag aatcggcaac gaggcgtgaa tatcatgacg caggcggatc
    11041 gccaccacaa acaccgccag atagcaaatc aaaaaggtgg cgctggcaac gctggcgagt
    11101 gagcctaaat ttagtgccgc cgtcatcagc ataatcaaca ccacgacaat gatgttgccc
    11161 caggtactct gccgccacag ggatttattc attagcttcg gcagttcgcg ttcgctgccc
    11221 atgttgtcca tgatgttaaa cacggcgaac aggttcgcgt taatggctga agccgtcgcc
    11281 agtaaagcgc cgatgacgac gatcacataa cccacatgtc cgagcagcgg agaagcagcc
    11341 tgcgctacgg cggtatcggc atatttttct aactctaatg ccgatacatc gctaagcaaa
    11401 accagtgcca gcgagatata aagcaacgtg gtaacgccaa tcgccaccag aaacgcccgc
    11461 ggcataatga cctgcggatc tttcacttta tccgccgcgt tcgccatcat gccaaagccc
    11521 gcataggcaa ggaaggttat cccgatacag gagaagaacg caccggagct ggggggcgca
    11581 gagacggaaa tatgcgccgg ttgcagcgac cagacaccgg caataatcag caataacagg
    11641 atcatcattt taatgccgac gaggatcact tccagccgcc ctaccgcatg gttgcttaag
    11701 gagttgaaaa gcgtcatcac cgcaatgatc cccaacgcgt agagcaaaat aaggtgctcc
    11761 tcctggctgc cttcatgcaa aaactgcacg gcataagcgc caaaagcacg ggcgaccatg
    11821 gcgatgctca ccgccagcgt caacaggtac agtaacgaga gcgccagcga aaagacgccg
    11881 ttgcctaatc cgcgacgaaa gaagtcgata atgccgccat tgctgggata gctcgccccc
    11941 agacgcgcat aggcataacc ggaaaacatc gccacaatac cgccaaaagc aaaggcgacc
    12001 caggtcgagg cttccattag caatgcagcc tgccccagca gcgcgaagat ccccgccccc
    12061 accattgccc caatgccgat ggaaacgacg ttccatagac cgagaggttt gttaccgtta
    12121 ttaccttccg tgttcatcat gatcagccct taaacacgtt atagccgagt tgcttagcga
    12181 ccgatgccac cattttttga ccgcgaacac tgttgccttc ttcgtccagc ggtggtgaga
    12241 acgcggcaat tcccatcacg ccagggacga ccgccagaat accgccacct acaccgcttt
    12301 tgcccggtaa accaacgcga tacgcccagt caccggagcg accatacagc ccttccatca
    12361 tcatttcggc cagaatgtac ggcacgttgt cggcctgaag aacgcgttta tgcgtcagcg
    12421 gattcacacc acccgccgcc agcgtcgcgc caagcgttgc cagttcaaca gtattgatga
    12481 gcgtggagca ctgacgggtg tacacgtcac aggcttccat tgcatcacaa tagagatatc
    12541 cggcggagta cagcagccag gcaatggccc ggttatggaa gttggttgtt tgttccgact
    12601 ggttgacttc atcagagagc gctacctgct caccagccag ttgctgttgg atatgtaaaa
    12661 ttcgctgcca gcgttgttca atattttcag cgttaatcag gctggtggtg gcaatagcgc
    12721 cagcatttac cagtggcgac agtggtttgc cgccatgcaa ctctaaggcg ataactgagt
    12781 taaagggcaa tccggtcggg tcagcgccaa ttttgtcctg taccgcctgc gggccgacat
    12841 cttctaacgc aagggctaac gtacagactt tcgagatgga ttccagcgca aagcggtaat
    12901 cactatcacc cgcgcgatag atgttgccat cgcaggtcac aatagccact gctgccagtt
    12961 gactaggtac attcgccaga aagggaatgt agtcggcatt ttgtccgccg ttaagtgagt
    13021 gaaattgggt gtaagcctga tccactgcct gctgtaattt gtttgcatct aacatctttt
    13081 gttaactcct ttttatagat gcgggaggta attcctcacc ccggtgccga ttttcaggca
    13141 tcttgattta acttagcacc cgcaacttaa ctacaggaaa acaaagagat aaatgtctaa
    13201 tcctgatgca aattgagcca attttttaat ctttacggac ttttactcgc ctggtttatt
    13261 aatttcttga ccttcccctt gctggaaggt ttaaccttta tcacagccag tcaaaactgt
    13321 cttaaaggag tgttttatgt cacaaattat cgacctgacc ctggacggcc tgtcctgcgg
    13381 tcactgcgtt aaacgcgtga aagaaagtct cgaacagcgc ccggatgtgg aacaagcgga
    13441 tgtgtctatc actgaagcgc acgttaccgg gactgccagt gcagaacagc tgatcgaaac
    13501 catcaaacaa gcgggttatg acgcatctgt aagccaccca aaggctaaac cgctggcgga
    13561 gtcatcaatc ccgtcggaag cactgacagc ggtttctgag gcgcttccgg cagcgaccgc
    13621 cgatgacgat gacagccagc agttgctgct gagcggcatg agctgcgcca gctgtgtcac
    13681 ccgcgtacaa aatgcgctgc aaagcgtacc gggcgtcact caggcacggg taaacctggc
    13741 ggagcgtact gcgctggtga tgggcagtgc ctccccacaa gatttagtgc aggcggtgga
    13801 aaaagcgggc tacggcgcgg aagcgattga agatgacgcg aaacgccgtg agcgccagca
    13861 ggaaaccgcc gttgctacga tgaagcgctt ccgctggcag gcaattgtcg cactggcggt
    13921 gggtatcccg gtgatggtct gggggatgat tggcgataac atgatggtca ccgctgacaa
    13981 ccgcagcctg tggttggtta tcggcctgat aaccctggcg gtgatggttt tcgccggcgg
    14041 ccatttttac cgcagtgcat ggaaaagcct gctgaacggt gcagcgacga tggatacgct
    14101 ggtggcgctg ggtactggcg tggcgtggct ctattcgatg agcgtcaacc tgtggccgca
    14161 gtggttcccg atggaagcgc gacatcttta ttacgaagcc agcgcgatga ttatcggtct
    14221 gatcaatctc ggccatatgc tggaagcacg cgcacgtcag cgttcttcta aggcgctgga
    14281 gaaattactc gatttaaccc caccgacggc gcgcctggtt actgacgaag gtgaaaaaag
    14341 cgtgcctctg gcggaagtgc agccaggtat gttgctgcgc ctgacgaccg gcgatcgcgt
    14401 gccggtagat ggcgagatta cccagggcga agcatggctg gatgaagcga tgctgacggg
    14461 cgaaccaatc ccgcagcaaa aaggcgaagg cgaaagcgtc catgccggga cagtggtaca
    14521 ggacggtagc gtgctgtttc gtgccagtgc ggttggcagc cataccacgc tgtcacggat
    14581 cattcgcatg gtgcgccagg cccagagcag caagccagaa atcggtcagc tggcggataa
    14641 aatctcagcc gtatttgtac ctgtggtggt ggtgattgcg ctggtcagtg cggcaatctg
    14701 gtacttcttt ggtccggcac cgcaaattgt ttataccctg gtgattgcca ccacggtact
    14761 gattatcgcc tgtccgtgtg cgctggggct ggcgacgccg atgtcgatta tttccggcgt
    14821 cgggcgggcg gctgagtttg gcgtgctggt gcgggacgcc gacgcgctgc aacgcgccag
    14881 tacactcgat accgtcgtgt tcgataaaac cgggacgctg accgaaggga agccgcaggt
    14941 tgtcgcagtg aaaacgtttg ctgatgttga tgaagcgcag gcattgcgtc tggcggcggc
    15001 actggagcaa ggttccagcc atccgctggc acgggcgatc ctcgataaag caggtgatat
    15061 gcagctgccg caggtcaacg gtttccgcac attgcgcggg ctgggcgtga gcggtgaagc
    15121 tgaaggtcat gcgttattgc tgggcaatca ggcgctgtta aatgagcaac aggttggtac
    15181 caaagctatc gaagcggaga ttactgctca ggcatcgcaa ggggcaacgc ctgtgctgct
    15241 ggcggttgac gggaaagcgg tagccctgct ggcagtacgc gatccattgc gtagtgatag
    15301 cgtggcggcg ctgcaacgcc tgcataaagc gggatatcgt ctggtgatgt tgaccgggga
    15361 taacccaacc accgccaatg cgatcgccaa agaagccggg attgatgaag tgatcgccgg
    15421 ggtgctgccg gacggtaaag ccgaagcgat taaacgcctg caaagtgaag gacgtcaggt
    15481 ggcaatggtg ggcgacggca ttaacgatgc gccagcgctg gcccaggcgg atgtcggtat
    15541 tgcgatgggg ggcggcagtg atgttgccat tgaaaccgcg gcgattaccc tgatgcgcca
    15601 tagcctgatg ggcgttgcgg atgcgctcgc gatttcccgc gcaacgctgc gcaacatgaa
    15661 gcagaacctg ctcggtgcct ttatctacaa cagtatcggt attccggtcg ccgccggtat
    15721 tttgtggccg tttactggaa cactgcttaa cccggtagtt gccggagcgg caatggcgct
    15781 ctcgtcgatt accgtagtga gtaacgccaa ccggttgctg cggtttaaac cgaaggaata
    15841 agtatgtcat tgcggatgcg ctgctttagg gtggcacatt tggcatcgac gcatgactgc
    15901 cggatgcggc gtaaacgcct tatccggcct acgagatgaa gcgatgtttt ggccttacgc
    15961 gacctttttt gcacgctctt cacggcgtgc caagtattca gccactgttt caatccgtcc
    16021 caattcttcc tgggtgcgca tgttgttttc aacttcccaa agatcaactg ccgcctggag
    16081 gtttagccaa aaatcgactg tggtatcaaa aactttcgcc agacggaatg ccatctcagt
    16141 ggtgagttta cgattgttat tgatcagtgc actgacgcta ttacgatgaa catgcagcaa
    16201 ctctgctaac tcattgattt tcaaatcgag cggtaccaga tattcataga gaagaatatc
    16261 tccgggtgtc gtcggttttc ttgttgcctg tttcatagtt tttaactgtt gcttccctgt
    16321 gaatagcgat acaagaatat actgaatcat acaaacacat taaacaagtc cccaactcca
    16381 tttgcaccat ccctccccgc gcgctagcat gatatttcac aaagggaggt cgtatggatc
    16441 tgttgtaccg ggtaaaaacg ctttgggccg cgctgcgcgg taatcattac acctggcctg
    16501 ccatcgatat cacccttccc ggcaatcgcc attttcatct gattggcagt attcatatgg
    16561 gtagccacga tatggctccc ctgcccaccc gtttgctcaa aaagctcaaa aacgccgatg
    16621 cgctgatcgt cgaggcggat gtttccacca gcgatacgcc ttttgccaat ttgcctgcct
    16681 gcgaggcgct ggaagagcgc attagcgagg agcaactcca aaatctgcag cacattagcc
    16741 aggagatggg catttctccc tcactctttt ctacccaacc gctgtggcaa atcgcgatgg
    16801 ttcttcaggc gacgcaggca caaaaactgg ggctgcgggc agaatacggt atcgattacc
    16861 agctattgca ggcggcgaag caacaacata aacccgtgat tgaactggaa ggggctgaaa
    16921 accagattgc catgttgctc cagctccctg acaaaggact ggcgctgctg gacgatacac
    16981 tgacccactg gcacaccaat gcacggttgc tgcaacaaat gatgagctgg tggctgaatg
    17041 caccgccgca aaataatgaa ataacgctgc ccaatacgtt cagtcagtcg ctgtacgatg
    17101 tgctgatgca tcagcgaaat ctcgcctggc gggataaatt acgcgccatg ccgccggggc
    17161 gatatgtggt cgcggtcggt gcactacacc tgtatggaga agggaattta ccgcaaatgt
    17221 tgcgctaaaa aaatggccaa tatcgctatt ggcccgtcaa agaggaattt cattttttta
    17281 ttattatgcc gtcactttaa gcgacggtgt aactcgattg tcgctcaatc tcaccatcct
    17341 gccaatactc ttaggcaaga tgactctttt ttttggaccg ccatcaggcg tatgcttgct
    17401 tcgttttttt gtttaaggca ggaaaattac catgacaccc gcagttaaat tactcgaaaa
    17461 aaacaagatt tcgtttcaaa tccataccta cgagcacgat ccggctgaaa ccaattttgg
    17521 cgatgaagtc gtcaaaaaat taggtttgaa tccggatcag gtctacaaaa cgctgctggt
    17581 ggcagtgaac ggtgatatga aacaccttgc cgtggccgtt acgccggtcg ccggtcaact
    17641 ggatcttaaa aaagtagcaa aagcgctggg tgccaagaaa gttgagatgg ccgatccgat
    17701 ggtcgcgcag cgttcgacgg gatacctggt tggggggatt agcccactgg ggcagaaaaa
    17761 acgtctgcca acgattatcg acgcccccgc acaagaattt gccactattt atgtttccgg
    17821 cggcaagcgc ggactggata tcgaactggc ggcaggcgat ctggcaaaga tcctcgatgc
    17881 caaatttgct gatatcgccc gccgcgatta agcattgtgc cggatgcaaa catccggcac
    17941 tttcggatta ctgccagctc acctcacctt tcggttcata aacactcaca tccagcggcg
    18001 agcttttctg gatatacgct ttcagcactt cggcatcaat aaagccggta ttcacatagc
    18061 ccggtttgtt atcaaggcgc ggatatccat caccgccggt ggcattgaag tttaatgtcg
    18121 ccatacggta agttttcgcc ggatcgaccg gttcgccttt gattttaagg tcgttcagtc
    18181 tgccgtcttt cgccacaaag ctaacgttgg caaattgcgg gtaggcacct gaatctggct
    18241 tcatctgcgc gacggcggtc aggtaatcaa tcacctcttt accggtcatg tcggcataca
    18301 ccaccacatt gccgaatggc tgcactttca gcacgttttt atagctgata tcgcctgctt
    18361 cgatagaatc acgaattccg cctccgctca tcaccgcaaa gtcggcacca gtgcgatcca
    18421 tttgggctgc cagaatcaac cgccccatat tggtctgtac aaaacgcact ttgtcacgat
    18481 cgccttccag acgaccattg gtttcgccta ttttcacttc cagctgcgct ttgcctttgt
    18541 tctggaacgg tgataacagc gagatcattt gctggttttc agcgatttca ggagtgtaaa
    18601 gcacgcgctc gcttttcccg tcttcccagg tcactttctt cttcaggttc accggaatca
    18661 gctggtagtt aaccattttc attttgccat tacgaaactc aaaatcagcc cgtcccacgt
    18721 atttgcccca ctcatgcgcc tgcacaatcc agatgccgtt ttgttgatct ggcttgcatg
    18781 gcgtacccgg cacgtaatcg acctgttttt tgttttctgc cgccatgcag accggatctt
    18841 gcgagtgacc accgacgatc atcgccagcg atccggcagg cagcgcgcgt gccatctcca
    18901 catcgcccgg tgcgttagag ccgtgctcac cattatcgta atgccccata tgggtcgccg
    18961 cgataataat gtctggcttt tctgtctgtt gcagctcctg aatcaccagc ttcgcttcat
    19021 cggcgggctt acgaaattcg acatcagtga agtattccgg gttaccaatt tttgctgtgt
    19081 catcggttgt cagcccaatg acggcaattt tcagatcctg acgcttaaac agcgcccacg
    19141 gtttaaacag gcgctcgcca gtacttttct ggtagatatt cgcggaaagc aacgggaact
    19201 tggcccactt ttcctgctgg cgtaatacgg tgagcggatt atcaaattca tgattaccga
    19261 tcgccatcgc gtcatagccc accagattca taccgcgaaa atcaggttcg gcatcctgta
    19321 agtcagactc gggcacgcca gtgttaatgt cgccaccgga aagtagcagc acgctaccgc
    19381 cttcagccgc aacctctttg cggataccat ccaccagcgt tttttgcgcc gccagaccat
    19441 attcgccata ttcattgcgc caaaaatgcc catgatgatc attggtatgc agaactgtaa
    19501 ttttgtaggt tttatcctgc tcatacgcca gagcagtttc actcgccagt gtaaatgtgg
    19561 ttaacagcgc taacgccacg ccccgctgca ataatttcat acttctctcc ctgacctgat
    19621 ttcaacgtgt tgaaaaatag tatgacgcga catcatagac aagtctgcgt ccggtgaaga
    19681 taaatctgca tccagattag aaattctgta ttcggcgttg agacttcctt caggcgacgc
    19741 atacaggtat gttagtcggg taataatcac aaataagatc attctttaca tcgcaaccct
    19801 gaaaaagtga ctttcctatg gcaatgagtg aacaacccca gcctgtggcg ggcgcggctg
    19861 cgtcaacgac caaggcccga acatcgtttg gtattttagg tgctatcagc ctctcacatc
    19921 tgctgaacga catgatccaa tcgctgattc tggcgattta tccgctgctt cagtcagaat
    19981 tttctctgac atttatgcag attggcatga taaccctcac cttccagctc gcctcttcgc
    20041 tactgcaacc agtggtcggc tactggaccg ataaatatcc gatgccgtgg tcgttgccaa
    20101 ttggcatgtg ctttacctta agtggtctgg tgctgcttgc gctggcgggc agttttggcg
    20161 cagttctgct ggcggcggcg ctggtcggta ccggttcatc ggtctttcat ccggaatctt
    20221 ctcgcgtagc ccgtatggct tccggcgggc ggcatggcct ggcgcaatct atctttcagg
    20281 tcggcggcaa ctttggcagt tccctgggac ccttgctggc ggcggtgatt atcgcgcctt
    20341 atggcaaagg caacgttgcc tggtttgtgc ttgcggcact gctggcgatc gtggtgttgg
    20401 cgcaaatcag ccgttggtac tcggcacagc accgaatgaa taaaggaaaa cccaaagcga
    20461 cgattatcaa tccactgccg cgcaacaaag tggtactggc ggtcagcatt ctgttaatcc
    20521 tcattttctc gaaatatttc tatatggcga gcatcagcag ctattacacc ttttatctga
    20581 tgcaaaaatt cggattatct atccagaatg ctcagcttca tctgtttgcc ttcctgtttg
    20641 ccgttgcggc aggtacggtg atcggcgggc ctgtagggga taaaattggg cggaaatatg
    20701 tgatttgggg ctctatcctc ggcgttgcgc cgtttacgct gattttaccc tacgccagcc
    20761 tgcactggac gggggtttta acggtgatta ttggatttat cctcgcttcg gcattctctg
    20821 ccattctggt ttacgctcag gagctgcttc cgggacgtat cggtatggtt tctggactct
    20881 ttttcggttt tgcttttggt atgggaggtc tgggagcggc agttctgggg cttatcgccg
    20941 atcacaccag tatcgagtta gtctataaaa tctgtgcttt cctgccacta ttggggatgt
    21001 tgaccatatt cctgcctgat aaccggcata aagactgatt tcattgcagc caaaggcaaa
    21061 ctttggctgc atcgttttac agtcgccata agcctttcct ctgttaaacc gccttctgtt
    21121 ttccgatccg tgcttttttt gcagctaatt ccatttttct gcaaaattca ataattattc
    21181 atgaacatca tcaatgaaat tgtcataaac tattacctgt aattttggtt ttcccggcca
    21241 aaaatggaca ataaccacca ggaaaaggag acggaatgca tcacgccacc ccgcttatca
    21301 ccaccattgt tggcggcctt gtgctcgcct ttatcctcgg catgctggcc aataaactac
    21361 gtatttctcc tctggtggga tatctgttag cgggtgtgct ggcaggacca ttcactccgg
    21421 gctttgttgc cgataccaag cttgcgccgg aactggctga actgggcgtt attttgctga
    21481 tgttcggcgt cggtttgcat ttttcgctca aggatttgat ggcggtaaag tccatcgcca
    21541 ttcccggcgc gatcgcccag atagccgtgg cgacgctgct gggtatggcg ctctccgccg
    21601 tgctgggctg gtcgttaatg accggtatcg tgttcggttt atgtctttcc accgccagta
    21661 ccgtggtgtt actgcgcgca cttgaagaac ggcaattaat tgacagtcag cgtgggcaaa
    21721 tcgccatcgg ttggttgatt gtggaagacc tggtaatggt tctgacgctg gtgttgctgc
    21781 ccgcagtggc aggaatgatg gaacagggcg atgtgggctt tgccactctt gcagtcgata
    21841 tggggatcac catcggcaaa gtgatcgcat ttatcgccat tatgatgctg gtaggtcgcc
    21901 gtctggtgcc gtggattatg gcacgcagcg cggcaaccgg ttctcgcgag ctgtttaccc
    21961 tgtcggtgct ggcgctggcg ttagggattg cctttggtgc ggtagagctg tttgatgtct
    22021 cctttgcact cggtgcgttc tttgccggga tggtactgaa cgagtctgaa ctgagtcacc
    22081 gtgccgccca cgatacgctg ccattgcgcg acgcgtttgc ggtgctgttt tttgtctccg
    22141 tcgggatgtt gtttgatccg ttaattctga ttcagcaacc gctggcagtg ctggcgacgc
    22201 tggcgattat tctgtttggt aagtcgttag ccgcattttt cctggtgcga ctgtttggtc
    22261 actcccaacg tacggcatta accatcgccg ccagcctggc gcagattggt gagttcgcgt
    22321 ttatcctggc gggactggga atggcattga atttactgcc gcaggccgga caaaacctag
    22381 tactggcagg ggcgatcctg tcgattatgc tcaacccggt actgttcgca ctactggaga
    22441 aatatctggc gaagaccgaa acgctggaag agcagacgct ggaagaggca atcgaagaag
    22501 agaagcagat cccagtggat atttgcaacc atgcgctact ggtgggttac ggtcgtgtag
    22561 gcagcctgct gggggagaaa ttgctcgcct ctgatattcc gctggtggtg attgagacgt
    22621 cacgaacccg tgttgatgag ctgcgagagc gcggggtccg cgcagtattg ggcaatgcgg
    22681 cgaacgaaga aattatgcaa ctggcgcatc tggaatgtgc aaaatggctg atcctgacga
    22741 ttcccaacgg ttatgaagcg ggtgagattg tggcatctgc ccgcgcgaaa aatccggata
    22801 ttgagattat tgcccgcgcc cattatgacg atgaagtggc gtatatcacc gaacgtggtg
    22861 cgaatcaggt agtgatgggc gagcgtgaaa tcgcccgtac catgctggaa ctgctggaaa
    22921 cgccaccggc gggtgaggtg gtgacggggt aaacggtggt tgccggatgt aaaatatgaa
    22981 aaatatgtag gcatgataag acgcgccagc gtcgcatcag gcatcgagag ccaaatgcca
    23041 gatgcggcgt gaacgcctta tctggcctac gaaccgacga taacttaacg atcccagtaa
    23101 gactcttcca ggctgtcttc acgctccggc aagccgcgcg ttaaacgagg tgaatgctgg
    23161 ttcagtacct gatagctcac acggttagca tatttacaca cctgcgctaa cgatgaataa
    23221 gttaaccagg tgaatttatg tttgctggag tttggtacgt tgctacgatg gtagctgttg
    23281 gcggtaatgt catgcagcaa cgctgccaat gcgccatccc ccgctccatt agtgttcatg
    23341 attttttccg gcccgcccat gtacggcgca atgtgcgaat atacacgcag cggattctgg
    23401 caatccttgt ggcgcatggc gcggctaaac tcatactggt tgaattccgc tatagcgccc
    23461 ggcagcagcg gatgctgggt tttacgtttc gcttcgtctt cggtaaagcc cgccatatac
    23521 aagccgattg gcccggcggt gcacagcacc agatctaccc agtccagcgc cttgtcagat
    23581 gccaacaacg gatcgctttc tccggtcaac gcttcggctt catcttcgtt catcgcaagg
    23641 atagaaacgt ggtctttgag gaattgctgc caccactgcg gattctcggc aatgacaaac
    23701 ttggtgccca gcgtcagcac caccggtacg ttatatttct tcgcgtactc aatggctttc
    23761 atggttgctt ccggcatggg ttcacccggc ttgcaacgca ccagatatga ggtgagaacc
    23821 agtgccgagg ctccggcaat cacatcttcc ggaatgcttt cagcccgcag ctggttcatg
    23881 tggcctggac tgatagcaaa ggtacgttcc ccggactcgc caatcagcgt aaagcaacga
    23941 ccaatcgggc catccacgcc ttgtagatag ttaagatcgg tacggctgga agtgttacac
    24001 aggtaacgat aggcataact gccaatttca atattgctgc acatgacgcc cagcagcacc
    24061 gaacggtcgt ccgcgagcac cgagtagttg tgcatggtgt taccaatggt gccacccgca
    24121 aactgatggg taatcaggtt tttctgtttt agctcctgat aaagcgcttc ggctacgtca
    24181 tcctcaatca ccagtgaatg cccggcgctt aatccataac gctcaatgaa ttcatcatcc
    24241 actttcgctt caatatcgac cagcgtctgg tcaataccta cgacccaggc ggcgctggtt
    24301 tcgttttctg gctggaattg ctgaagcagc ggatcgcgtg catttacagg gaagtaatgt
    24361 ttggatttac gtttaccggg aaatttcatg gttgttttta cgggtggtta actgagcggc
    24421 gaatgttaac acaatctgac gggagcgcgc gagacgcagc cagtttcaat atcagcaggc
    24481 gggatcgcat tttcatggca gtggttcctt acaatgacgt aatttgaaag gagtttttgt
    24541 atgaagccgg aaaacaaact acctgttctg gaccttattt ctgctgaaat gaagaccgtt
    24601 gtgaatactc ttcagccgga tttaccgtcc tggcccgcaa cgggaacgat tgctgagcaa
    24661 cgacagtatt acacgcttga gcgccgattc tggaatgcgg gcgctccaga aatggcaacc
    24721 agagcttaca tggttccaac aaaatatggg caggtggaaa cacgtctctt ttgtccgcag
    24781 ccagatagcc cggcgacgct attttatttg catggaggcg gttttattct cggcaatctc
    24841 gatacccacg atcgcatcat gcgcctgctg gcaagctaca gccaatgtac ggtgattggt
    24901 attgattacc ccctttcacc tgaagcgcgt tttccgcaag cgatagagga aattgtggct
    24961 gcttgttgtt atttccacca gcaggcggag gattatcaaa tcaatatgtc ccgcattggc
    25021 tttgccggtg attccgcagg tgccatgctg gcgctcgcca gtgcgttgtg gttgcgtgat
    25081 aaacagatcg attgcggtaa aattgcgggt gttttgctgt ggtatgggct ttacggatta
    25141 cgggattccg tgactcgtcg tctgttgggc ggtgtctggg atggcttaac gcaacaggat
    25201 ttgcagatgt atgaagaggc gtatttaagc aacgacgcgg accgcgagtc accgtattac
    25261 tgtctgttta ataatgatct cactcgcgaa gttccgccct gttttattgc cggggcggag
    25321 ttcgatccgc tgctggatga cagccgtctg ctttaccaga cgttagcggc gcatcagcag
    25381 ccctgtgagt tcaaactcta cccaggtacg ctgcatgcct ttttgcatta ttcacgaatg
    25441 atgaaaaccg ccgacgaggc tcttcgcgac ggcgctcagt tctttaccgc tcagctttag
    25501 cgatacgcgg caacaagatt agccatcatt tcgatatgtt ccggcgtggc attaagcgct
    25561 ggaatatatt catatttttt cccgccggca ccgaggaaga cctcacggtt ttgctcggca
    25621 atctcttcca gcgtctccag acaatccgca gcaaagcccg ggcacatcac ctgtatatga
    25681 cctacgcctt tttctccgag cattttcagc gtttcgtcgg tataaggcat cagccagggt
    25741 tcccggccaa agcgcgactg aaaggtcatc atcacttttt ccggtgccat ccccagtgcg
    25801 gaagccagtt cgcgagtcgt tgtgcggcaa cgttgcgggt aatcatcgcc ttcatctgca
    25861 taacgctggg gaatgccatg ataagagagc agcagcagat ccggttcgcc atgtttggca
    25921 aaagaagcgc gtacgctgtt cgccagtgca ttaatgtaat cgtggttatc ggcgtaatca
    25981 cgaataaacg atatccccgg aatgctacgt ttgcgcgcca gaatgcgtgc cagttcatcc
    26041 cataccgcac cgaccgtaga acaggagaat tgcggataaa gcggcagcac cacaatatga
    26101 tctacatgct ctgccaggag ttcatcgacg gcgctttcca gtgatggcga gccgtagctc
    26161 atgcccagcg ctacgggcgt atccggtaaa cgttgtgcca acgcctgctg ttgctggcgg
    26221 ctgtaaacca tcagcggcga gccgtcttcc atccagaccg aggcgtacat cttcgccaca
    26281 cgcggcgagc gcagcggcaa aatcacgccg cgcagcaacg gccaccataa caaccgtgag
    26341 gtatcaacca cgcgtctgtc gcttaaaaat tgtttcagat agcgttttac cgcttcaggt
    26401 gtgggggcat cgggcgtacc caggtttgcc agcaggatac cggttttagt ctgacgcatt
    26461 accgcctctt atcgattcaa cttgttgata attgtagcgg aaaagtgagg aagaagaact
    26521 aattgttgtg agaggtcaaa tcagaaaaag gaatagcgca gattgaggtt tttgtaaaag
    26581 gccgaatgaa tatccggcct gagattgctg ataagtttgc ttgtgcgggc ctgttcccct
    26641 caccctaacc ctctccccga gggggcgagg ggactgtccg tgcgcgcttt cgaattagcc
    26701 gaggattttt tccagatcag cgcgaacttc agcaaccggc ttggtgccgt caactttcgc
    26761 gtatttggtg ttacccgctt ccgcttcttt ggagtagtag ccgatcagcg gtgcagtcat
    26821 ctgatggtat tcaaccagac gtttacgcac ggtttcttcc tgatcgtctt tacgggtagt
    26881 cagttcttca ccggtaacgt cgtctttgcc ttctactttc ggcggattga atttaacgtg
    26941 ataaacacga ccagacggcg catgaacgcg gcggcctacg atacgatcaa caatcagttc
    27001 gtccggtacg tcgaattcca gaacgtaatc aacattgatg cccgcttctt tcatcgcgtc
    27061 tgcctgcgga atggtacgcg ggaagccgtc caacaggaaa ccattacggc agtcttcctg
    27121 agcaatgcgc tctttaacca gcgcgatcac cagttcgtcg gtgaccaatt tgccagcatc
    27181 cataatgtct tttgcttgtt tacccagctc ggagccagat ttgaccgcag cacgcagcat
    27241 atcgccagtg gagatttgcg gaataccata tttctccatg atgaactgag cctgagtccc
    27301 tttccccgcg cccggagcgc caagcagaat gatacgcatt gcgaaaatcc ccttaaaatg
    27361 tgtcgaattt tttgaaaaag cgataaacga taccaccaac aggcgattgc ctcaagaaag
    27421 gcacctgagg ctgaaacggt taatggcgag ataatttgcg gaaagtgcag atgatgaaaa
    27481 gaaaaatgcc ggatgacaca aaggtcatcc ggcatgacat caggaaacca gcagctggtt
    27541 catacgacga ataaacaggt tcggatcttc cagcgtgccg cgttctgcca gcagcgcctg
    27601 atccagcagc agttctaccc actcgctgaa cttcgcttca tcttcagtat ctgccgcacg
    27661 tttcaccagt acgtgatccg ggttcagttc gaagatgtat ttcacttctg gcactttctg
    27721 gcccgccgca gcgaacagtt tcgccatctg agtgctcatt tcgtccgcgt cggtcgaaac
    27781 gatcgctggc gtatcggtca gacggtgagt cagacggaca tctttcacgc gctcgccgag
    27841 cagggctttc acacggtcga tgaacggagt cagtgctttc tccgcttctt tcgcgctctc
    27901 atcaacttcg tcagccagtt tttccagcga ctcgtcaact ttagacaccg actggaacgg
    27961 tttaccgtcg aactcagtca gatagttcat catccactca tcgatgcggt cggaaagcag
    28021 cagaacttcg atgcctttct tacgcagcag ttccaggtgc gggctgctct tcgctgccgc
    28081 atagctgtct gcagtgatgt agtagatttt ctcctgccct tctttcatgc gggaaacgta
    28141 gtcttccaga gacacggtct gcgcagaaga atcggtataa gtagaagcaa aacgcagcag
    28201 tttggcgatc gcttcctggt tagcgaaatc ttccgccgga ccttctttca gtaccaggcc
    28261 aaactgttgc cagaaggtct ggtatttgtc cgcgtcgtct ttcgccagtt tttccagcat
    28321 ttgcagcaca cgcttggtca gcgcattgcg caggttacgc gttaccgtgc tgtcctggag
    28381 gatttcacgg gaaacgttca gcggcagatc gctggagtca atcagaccac gcacgaagcg
    28441 cagatagttc ggcatgaact gttctgcgtc gtccatgatg aacacacgct ggacgtacag
    28501 tttcaggccg tgtttatgat cacggttcca catatcccac ggagcctggg acgggatgta
    28561 cagcaggctg gtgtactcct gcttaccttc aacgcggttg tggctccagg tcagcggatc
    28621 attaaagtcg tgggcgatgt gtttgtagaa ctctttgtac tcttcatcgg tgatttccga
    28681 cttgttacga gtccacagcg cctgcgcttt gttgattttc tcccaggaga taacggtttc
    28741 gccgtctttc tcttcgcgtt tttcgatctc taccggcagc gcgatatggt cggagtattt
    28801 gctgatgatg gaacgcacgc gccagtcatc gaggaactcg tcttcgcctt cacgcagatg
    28861 cagggtgatt tcagtaccac gatcttcttt ggtgatgtcg gcaacggtat attcaccttc
    28921 gccagccgat tcccagaaga cgccgttttc tggtttttcg cctgccgcgc gagtacgcac
    28981 ggtcactttg tcggccacga taaacgcaga gtagaaacca acaccaaact gaccgatcag
    29041 ctggctgtct ttcgcctggt cagaacccag ggattcgagg aatgatttgg taccggattt
    29101 agcgatagtc cccagatggt caatcacttc gtcgcgggtc atccccacgc cgttatcgga
    29161 gatggtcagc gtgcgcttgt ctttatcgaa agagacacga acgcgcagtt cgccatcacc
    29221 ttcgtacagg tccgggttag atagcgcacg gaaacgcagc ttgtccgccg catcggaggc
    29281 gttagagata agctcacgca ggaagatttc tttattggaa tagagagaat ggatcatcag
    29341 gtgcagaagc tgtttcactt ctgactgaaa accacgagtt tcttgtcctt tcatgtaggt
    29401 ctacctcaat aatgccattt taaggttaaa aacaggatga gggagagatg gggacaaggg
    29461 agaataattt caagcgagag caggtgatcc tgctctcgtt tgcttaaaaa cgaatcttat
    29521 gacgcccggc aagggagtgt gacagcgtgg tgccatcgac catttccagt tcgccgccaa
    29581 ccggtacgcc atgagcgatt cggctggctt ccacgtcata ttgcgcgcaa agctcggcaa
    29641 tgtagttagc ggtagcttca ccttcaaccg tgggattggt ggcgaggatc acttcagtga
    29701 ttttttcctc tgccagacgc tgttccagac gatcaaggcc gatatcatcc ggaccgatgc
    29761 cgtccagcgg tgacaggtgc cccatcaaca caaaataacg gcctgaaaac tgccccgtct
    29821 gctcaatggc gtagatgtcc gccggactct ccaccacaca gatttgaccg ttttcctgac
    29881 gacgcggatt cgaacagatg ttacagactt cctgttcggt gaaagtgcgg caatcggcgc
    29941 agtggccgat ttccgacatc gcccgggtga gcgcctgcgc cagacgcatc ccgccgctac
    30001 gatcgcgctg aagcagcgtg aacgccatac gctgcgccga cttcgggcca acgcccggca
    30061 gacagcgcag tgcttccata agctgtgtta acagcgggct ggtttgcatc agaacggcat
    30121 cttaaagcca ggcggcagct gcattccgga ggatacagag gccatttttt ctttctgcgt
    30181 ttcttcaata cgacgtgctg cgtcgttgaa tgctgcagcc accaggtctt ccagcatctc
    30241 tttgtcgtct tccagcaggc tcgggtcgat ctctacgcga cggcagttgt gtgcaccgtt
    30301 gatggtcact tttaccagac ctgcgccaga ttcgccggtg acttccagct gcgcgatctc
    30361 ttcctgcatt ttctgcattt tttcttgcat ctgctgggct tgcttcatca ggttacccag
    30421 accgccttta ccaaacatag gtttctctct caatcacgtt aaggatgacg aacgtaagct
    30481 gtgcttacga tcaaatgggg cggatacttt cttcatccag ctccgcatcg aagaaccgac
    30541 gcagggtctg aatattatta tccgcaataa tggactcgcg cgcctgcgca agtttttctt
    30601 cgtatatcgc ctgacgccac tccagcggcg tacgcaccgc gggattatca tcttcaacga
    30661 tagtcagttc aaccgttgaa ccttttaacg tgctcaacgc ttcagccagt ttttgctgtg
    30721 caccgcggtt gttcaaatgc cgctgagagg agcgcaaatg cagacatact gcgttgtcgc
    30781 tctcctcttt ccaggcattt aaggccacct gttcgaccag ttttggtagc gaaagttgac
    30841 tcacctgtgc cgcccacgcg tcgcgctcaa tggcttctgc cgccagcttc gccgccagtt
    30901 ccggcgtttt ttcatgttcc agcgcttttt tcagcgcctt cggcgtggcg accacttctt
    30961 tttgctgcat cactggagtg gtagccttcc agcgatacgc ttcttttttg gctggcgctt
    31021 tttccagcgc cgatggcacc ggacgcgcct gaacgcgatc ggtgaccgaa gccagtcttt
    31081 ccagcgcagc gttattcacc ggccgcgcgc gggtagcggc tgccggttca ctcttttttg
    31141 ctttggttgc tccctgcacg cgctgcaact gctggcgcgc cgccagcacc tggctggtgg
    31201 tttccgggag cggtacagtc ggtgcctgct gcggcgctga ttgcggttgc ggcggcacct
    31261 gggttggcgt cattactgcc gttggcgcga cgggcgcaaa ggactggcgt ggcacttctg
    31321 gctcaggcag aggcatacgt ggatggaacg ccagcgcgcg cagcagcgtc atctcaacgc
    31381 ccatgcggcg gtccggcgca tacggtaatt ctttgcgacc aatcaacagc gtctgatagt
    31441 aaagctgaat atccgtcggc ggtatggtgc gcgccagttc acgcatccgc agctcaatgg
    31501 cggccatgtc gttgccaagt gcagcaggcg aaagttgtac catcgcaata cgatgcaaca
    31561 ggccgagcat ttccaccagc aacgcttccc actcgatacc acgggcagcg gcttcattaa
    31621 tcagcgccat tacgcgctcg ccgttggcct cgaccatcgc ttcaaccagc gacagcgcct
    31681 gatcgtcgtc aagcgtaccc agcatcgcac tgactgcctg ggttgaaacc tggccgtcac
    31741 cgctggcaat cgcctggtcg gtcagactta aggcatctcg caggctgcct tcagcggcgc
    31801 gcgccagcaa ttgcaacgcc cgcggctcgt gagcgatatg ttcttcgttg aggatgtgct
    31861 caagttgatg gcgaatttgc tcgacatcca gcgccttgag atgaaattgc aaacagcgcg
    31921 acaaaatcgt caccggcaat ttctgtggat cggtcgtcgc cagcagaaac ttaacgtgct
    31981 ccggcggctc ttcaagggtt tttaacagtg cgttaaagct gtggcgcgac agcatatgca
    32041 cttcgtcgat cagataaact ttgaaacgac cacgcgccgg agcgtactgg acgttatcca
    32101 gcaggtcgcg ggtatcttca actttggtgc gcgaggcggc gtcgatttca atcagatcga
    32161 caaagcgccc ctgctcgatt tcacgacagt tatcgcacac gccgcacggc gtcgcggtaa
    32221 tgccggtttc gcagtttagc cccttcgcca gcagtcgggc gatagaggtt tttccgacgc
    32281 cacgggtacc ggaaaaaaga taagcatgat gaatacgccc taacgacaag ccgttcgcca
    32341 gtgcggtcag cacatgttcc tggccgacga cgtcagcaaa ggtttgtggg cgccattttc
    32401 gggctaagac ctgataactc attggcaggc tctgaaacgc tggaaggtgg attcacgaag
    32461 gggtaatgct aacacagccc cgtcagaacg gcgaggctga atgcacatct tgtgctgtcc
    32521 gtagcgtggg cagcacaaga ctggcgataa ttaatggccc gggaacggga caaggctgta
    32581 gctggtaatg ccctgttttt cgagacgctg ttcgccgccg agatcgaaca ggttgataat
    32641 gaacgcagcg tcagccactt caccacccag acgacggatc agtttaacgg tcgcttcgat
    32701 agtgccgccg gttgccagca gatcgtccac caccagaact ttgtcgcccg gtttgatggc
    32761 atcaacgtgg atctccagct gatcggtgcc gtattccagg tcgtaagttt cactgatggt
    32821 ttcacgcggc agtttgcccg gtttacggac cggtacaaag ccaacgccca gacccagagc
    32881 taccggagcg ccaaacaaga agccacgcgc ttcggtgccg acaactttgg taatgcccgc
    32941 atttttgtaa cgctcaacca gcaagtcgat gctgagagcg taagctttcg ggtcttccag
    33001 taagctggtg acatcgcgga aaagaatgcc gggttttggg tagtcctgaa tgcttttgat
    33061 gctatttttg agatactcaa gctgctgtgc agtcgcggtc ataagtgtat gcctgcttgt
    33121 tacggtggta ctcacggcgc gttttaaacg tatcaaaagt aacagttgtt taacctttga
    33181 ccgcctgcgc ctgtgctcga aaacgccaga atgtactggc tgtacgcaat aattgcaact
    33241 gcgattgttg tgcttcagtg cttttcttgc ttttcatcaa tcaccggaat tcgccacata
    33301 tagaaaagca aacaggcgag aattaccagc aacatgatgc gcacccatgg catctggacg
    33361 aaccacagag aaatggcaaa cgtgagcaaa ataagcaaaa tcgcccgcgg tttgacgccg
    33421 cgcggcatcg catgatgttt ctgccagaaa cgtagatagc tgccaaacca tgagcggtac
    33481 agcaaccagg cgtgaaagcg cggggaagaa cgggcaaagc accaggccgc cagcaggata
    33541 aacggcgtcg tcggtaatac cggtaatacc acgcccagcg tacccagcac taccgccagc
    33601 cagccaatga tgattaaaat gattcgttgc atattgagtg ttgtcagctt acagagtggc
    33661 tactttagca taacaattat cattttcatt gaggtcttat cgtgaaaacc gccctgctgc
    33721 tggaaaaact ggaaggacag ctcgctacgc tgcgtcagcg ttgtgccccg gtagcgcaat
    33781 ttgccacgct aagtgcccgt tttgacagac acctttttca aacccgtgcg acaacactgc
    33841 aggcttgtct cgatgaggcg ggcgataatc tggctgcgct tcgtcatgca gttgagcagc
    33901 aacagctgcc gcaagtggcc tggctggcgg aacatctggc ggcacaactg gaagccatcg
    33961 cgcgtgaagc ctccgcctgg tcattgcgcg agtgggacag tgcgccaccg aaaattgccc
    34021 gctggcagcg taaacgtatt cagcatcagg attttgagcg gcggctacgt gagatggttg
    34081 ccgaacgcag agcccgcctg gcgcgggtga ccgatctcgt ggaacagcaa acgctgcatc
    34141 gtgaagtgga agcctatgaa gcgcgcctgg cacgctgccg ccatgcgctg gaaaaaatcg
    34201 aaaacaggct agcgcgctta acccgctagc aatggagaga gtatgtcact ggaaaatgcc
    34261 cctgacgatg tcaaactggc tgtcgatttg attgtgctgc tggaagaaaa tcagatccca
    34321 gcccgcaccg tgctgcgcgc gctggatatt gtaaaacgcg attatgaaaa gaaattaacg
    34381 cgcgatgatg aggcggaaaa gtgagaaata aatggggccg ctaaatgtcc cctaagattg
    34441 tgggcaaacg caaaac
//
LOCUS       DAZFDP010000031        34408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFDP010000031 DAZFDP010000000
VERSION     DAZFDP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..691
                     /locus_tag="ACHJ1G_001671"
     CDS             <1..691
                     /locus_tag="ACHJ1G_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4763543.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     QIARLAEQSTD"
     gene            790..1803
                     /gene="ybjS"
                     /locus_tag="ACHJ1G_001672"
     CDS             790..1803
                     /gene="ybjS"
                     /locus_tag="ACHJ1G_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4763544.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1800..2630)
                     /gene="amiD"
                     /locus_tag="ACHJ1G_001673"
     CDS             complement(1800..2630)
                     /gene="amiD"
                     /locus_tag="ACHJ1G_001673"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4763545.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2627..2950)
                     /gene="ybjQ"
                     /locus_tag="ACHJ1G_001674"
     CDS             complement(2627..2950)
                     /gene="ybjQ"
                     /locus_tag="ACHJ1G_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4763546.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3076..3591
                     /gene="ybjP"
                     /locus_tag="ACHJ1G_001675"
     CDS             3076..3591
                     /gene="ybjP"
                     /locus_tag="ACHJ1G_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4763547.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3809..4537
                     /gene="artP"
                     /locus_tag="ACHJ1G_001676"
     CDS             3809..4537
                     /gene="artP"
                     /locus_tag="ACHJ1G_001676"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4763548.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4555..5286
                     /gene="artI"
                     /locus_tag="ACHJ1G_001677"
     CDS             4555..5286
                     /gene="artI"
                     /locus_tag="ACHJ1G_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4763549.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5293..6009
                     /gene="artQ"
                     /locus_tag="ACHJ1G_001678"
     CDS             5293..6009
                     /gene="artQ"
                     /locus_tag="ACHJ1G_001678"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4763550.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6009..6677
                     /gene="artM"
                     /locus_tag="ACHJ1G_001679"
     CDS             6009..6677
                     /gene="artM"
                     /locus_tag="ACHJ1G_001679"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4763551.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6969..7700
                     /gene="artJ"
                     /locus_tag="ACHJ1G_001680"
     CDS             6969..7700
                     /gene="artJ"
                     /locus_tag="ACHJ1G_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4763552.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7875..9002)
                     /gene="rlmC"
                     /locus_tag="ACHJ1G_001681"
     CDS             complement(7875..9002)
                     /gene="rlmC"
                     /locus_tag="ACHJ1G_001681"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4763553.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(9043..9531)
                     /gene="ybjO"
                     /locus_tag="ACHJ1G_001682"
     CDS             complement(9043..9531)
                     /gene="ybjO"
                     /locus_tag="ACHJ1G_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4763554.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9591..10436)
                     /gene="potI"
                     /locus_tag="ACHJ1G_001683"
     CDS             complement(9591..10436)
                     /gene="potI"
                     /locus_tag="ACHJ1G_001683"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4763555.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(10433..11386)
                     /gene="potH"
                     /locus_tag="ACHJ1G_001684"
     CDS             complement(10433..11386)
                     /gene="potH"
                     /locus_tag="ACHJ1G_001684"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4763556.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11396..12529)
                     /gene="potG"
                     /locus_tag="ACHJ1G_001685"
     CDS             complement(11396..12529)
                     /gene="potG"
                     /locus_tag="ACHJ1G_001685"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4763557.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12624..13736)
                     /gene="potF"
                     /locus_tag="ACHJ1G_001686"
     CDS             complement(12624..13736)
                     /gene="potF"
                     /locus_tag="ACHJ1G_001686"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4763558.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14087..14563)
                     /gene="ybjN"
                     /locus_tag="ACHJ1G_001687"
     CDS             complement(14087..14563)
                     /gene="ybjN"
                     /locus_tag="ACHJ1G_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4763559.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14651..15553)
                     /gene="rimK"
                     /locus_tag="ACHJ1G_001688"
     CDS             complement(14651..15553)
                     /gene="rimK"
                     /locus_tag="ACHJ1G_001688"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4763560.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15614..16336)
                     /gene="nfsA"
                     /locus_tag="ACHJ1G_001689"
     CDS             complement(15614..16336)
                     /gene="nfsA"
                     /locus_tag="ACHJ1G_001689"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4763561.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16320..16607)
                     /gene="ybjC"
                     /locus_tag="ACHJ1G_001690"
     CDS             complement(16320..16607)
                     /gene="ybjC"
                     /locus_tag="ACHJ1G_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4763562.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16767..17024
                     /gene="grxA"
                     /locus_tag="ACHJ1G_001691"
     CDS             16767..17024
                     /gene="grxA"
                     /locus_tag="ACHJ1G_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4763563.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(17054..17431)
                     /gene="ybjM"
                     /locus_tag="ACHJ1G_001692"
     CDS             complement(17054..17431)
                     /gene="ybjM"
                     /locus_tag="ACHJ1G_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4763564.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17701..19386
                     /gene="ybjL"
                     /locus_tag="ACHJ1G_001693"
     CDS             17701..19386
                     /gene="ybjL"
                     /locus_tag="ACHJ1G_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4763565.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19561..20097)
                     /gene="rcdA"
                     /locus_tag="ACHJ1G_001694"
     CDS             complement(19561..20097)
                     /gene="rcdA"
                     /locus_tag="ACHJ1G_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4763566.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            20181..21389
                     /gene="ybjJ"
                     /locus_tag="ACHJ1G_001695"
     CDS             20181..21389
                     /gene="ybjJ"
                     /locus_tag="ACHJ1G_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763567.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21389..22204
                     /gene="ybjI"
                     /locus_tag="ACHJ1G_001696"
     CDS             21389..22204
                     /gene="ybjI"
                     /locus_tag="ACHJ1G_001696"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4763568.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            22290..22574
                     /gene="ybjH"
                     /locus_tag="ACHJ1G_001697"
     CDS             22290..22574
                     /gene="ybjH"
                     /locus_tag="ACHJ1G_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4763569.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22615..23847)
                     /gene="mdfA"
                     /locus_tag="ACHJ1G_001698"
     CDS             complement(22615..23847)
                     /gene="mdfA"
                     /locus_tag="ACHJ1G_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO4763570.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            24132..24728
                     /gene="ybjG"
                     /locus_tag="ACHJ1G_001699"
     CDS             24132..24728
                     /gene="ybjG"
                     /locus_tag="ACHJ1G_001699"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4763571.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24786..25544
                     /gene="deoR"
                     /locus_tag="ACHJ1G_001700"
     CDS             24786..25544
                     /gene="deoR"
                     /locus_tag="ACHJ1G_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4763572.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(25591..26793)
                     /gene="dacC"
                     /locus_tag="ACHJ1G_001701"
     CDS             complement(25591..26793)
                     /gene="dacC"
                     /locus_tag="ACHJ1G_001701"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4763573.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            27040..27666
                     /gene="gstB"
                     /locus_tag="ACHJ1G_001702"
     CDS             27040..27666
                     /gene="gstB"
                     /locus_tag="ACHJ1G_001702"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4763574.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27663..28778)
                     /gene="yliI"
                     /locus_tag="ACHJ1G_001703"
     CDS             complement(27663..28778)
                     /gene="yliI"
                     /locus_tag="ACHJ1G_001703"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4763575.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28889..29272)
                     /gene="bssR"
                     /locus_tag="ACHJ1G_001704"
     CDS             complement(28889..29272)
                     /gene="bssR"
                     /locus_tag="ACHJ1G_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4763576.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29485..30810
                     /gene="rimO"
                     /locus_tag="ACHJ1G_001705"
     CDS             29485..30810
                     /gene="rimO"
                     /locus_tag="ACHJ1G_001705"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4763577.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(30857..32185)
                     /gene="dgcI"
                     /locus_tag="ACHJ1G_001706"
     CDS             complement(30857..32185)
                     /gene="dgcI"
                     /locus_tag="ACHJ1G_001706"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4763578.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32193..>34408)
                     /gene="pdeI"
                     /locus_tag="ACHJ1G_001707"
     CDS             complement(32193..>34408)
                     /gene="pdeI"
                     /locus_tag="ACHJ1G_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4763579.1"
                     /translation="RFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKM
                     EMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKR
                     PWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNE
                     YLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVP
                     NNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVN
                     TLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGF
                     IKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDI
                     VTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYA
                     EKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDI
                     NTRAILGAEALCRWVSAVRGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQR
                     ASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFY
                     LNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIF
                     DLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         8623 a         8902 c         8389 g         8494 t
ORIGIN      
        1 caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg ggctgaaaca
       61 cgatctgctg gcggatgata ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt
      121 cgatgacgcg gtacgtagca cgttgaaaga ggaggaaaaa ctggtcaact ccagcgactg
      181 gggctacgac gctcaggcct ttgcccgctg gcgaccagag tacggttatt ttgccaaaca
      241 ggcggggttt accgttaaaa cgtccgccag ccttgctgct ttatggcagg tagtgaacca
      301 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
      361 ggaccgcgcg atcggtcata agctggcgaa aggccgcccg gagcgcgaat atttacagac
      421 tggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt
      481 gttatttggc atgaaagcgc cggggctggg acgactgtgt tttagcctgg aagataaagg
      541 cgactatagt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
      601 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacaaat
      661 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
      721 ttcccattgc atcatggggg aaatcacgga agaatgcgca cgttattttc tcaacaacag
      781 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
      841 gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggc
      901 aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga gctggtttca
      961 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
     1021 tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg
     1081 ggtgaatgga ctgtcgcctg gggtgtgcgt aactttattc atatctcttc cccctccctg
     1141 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
     1201 aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct ttcgcaggcg
     1261 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
     1321 gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct gttaccgcat
     1381 ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc aatgtggctg
     1441 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat caccaacggc
     1501 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
     1561 cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat ggagcgttta
     1621 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa gttgaatttt
     1681 gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc ggtcatcacc
     1741 ctggatgaag gtatcgagaa aactgccgcc tggctacgcg accacggaaa actgccgcgc
     1801 taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc tgcatccgct
     1861 tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac gcgccgctgc
     1921 tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag ctccagcaat
     1981 gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt aacccgctgc
     2041 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
     2101 ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg
     2161 tgataacggg caataatatc tttcgccagt ggaataagcg cctgaatctg tgccggttca
     2221 aacggggcaa aatatttcac tccggccgat ttttgccagc cacggttttc cagctcaatg
     2281 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
     2341 gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta tcgcggtggt
     2401 accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta
     2461 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
     2521 gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgca
     2581 caccctgcca gtaacagagc ggcagcgatc agccagaaga ctcttctcat cgacgcgttt
     2641 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
     2701 agtcgatatc aataccgacg acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt
     2761 cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg gaacgtccgc
     2821 caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct aaaatcgctt
     2881 cgccggtcac cacaccgcaa tattcaacga tgatctggcc ttccagagtt ggggttgtag
     2941 aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc attatcagac
     3001 tgatacgcta ttattgaaag gatatcatta ttatcgtaga acgctttcag agcgatcgct
     3061 tataaggaaa tcattatgcg ctacagcaaa ttgacaatgc ttatcccctg cgcactgctc
     3121 ctcagtgcct gcaccacagt cactccagct tataaagata acggcacacg cagtggtcct
     3181 tgcgttgaag gaggcccgga taacgtggcc cagcaatttt atgactaccg cattttgcac
     3241 cgcagcaacg acatcaccgc cttgcgccct tatctgagcg acaaactggc gacactgctt
     3301 agcgatgcct cccgggataa taaccatcgg gaactactga ccaacgatcc attctccagt
     3361 cgcacaacct tgccagagag cgcccgtgtt gccagtgcct caacaatccc gaatcgtgat
     3421 gcgcgaaata ttcctctgcg tgtggatcta aaacagggtg atcagagctg gcaggatgaa
     3481 gtgttaatga ttcaggaagg ccagtgttgg gtgatcgacg atgtacgcta tttaggcggc
     3541 agcgttcatg ccacggcggg tacgctccgt cagtccatcg agaatcgcta acgccttgat
     3601 caaccaataa accccgtaaa ttgtccggca tttcgtgagg aatgccgaca tttatgctcg
     3661 ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt tgcttttaac
     3721 ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg acctcaatag
     3781 ctatcagact gccagtatac gagtgtcaat gagtattcaa ttaaacggca ttaattgctt
     3841 ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg gcgaaacgct
     3901 ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct
     3961 tgagatgccg cgctccggta cgctcaacat tgcaggcaac catttcgatt tcaccaaaac
     4021 accctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata
     4081 caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct gccgtgtact
     4141 ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct
     4201 caaaccttat agcgatcgtt acccgctgca tctttctggt ggtcagcagc agcgtgttgc
     4261 tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc
     4321 actggacccg gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa
     4381 tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt
     4441 ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac gcgagctgct ttaccgagcc
     4501 gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac aacaatgaaa
     4561 aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc tgccgaaacc
     4621 attcgttttg ctaccgaagc atcctatcct ccgtttgaat cgattgatgc aaacaaccag
     4681 atcgttggtt ttgacgtcga cctggcacaa gcgctgtgta aagagattga tgcaacctgc
     4741 actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa
     4801 gccgtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtgct gtttaccacc
     4861 ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat
     4921 cagctgaaag gcaaaaaagt cggcgtacag aacgggacga cacaccagaa attcattatg
     4981 gataagcacc cggaaatcac taccgttccg tatgacagct accagaacgc aaaactggat
     5041 ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg
     5101 aaagataacc cgaaactggc ggcggtgggc gacaaagtga ccgataaaga ttacttcggc
     5161 actggcctcg gcatcgcggt acgtcagggc aacactgagc tgcagcagaa actcaacact
     5221 gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa atggttccag
     5281 aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat gaccgtcggc
     5341 cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc ggtatgggag
     5401 tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc
     5461 ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct gctgctgacg
     5521 ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccagtgca gatggacatt
     5581 gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc
     5641 gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag
     5701 tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt gatgccgcag
     5761 atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc tggtgctgct gaaagatacc
     5821 gcgctggtca gtttgattag tgtgaatgat ttaatgctgc aaacaaaaag catcgctact
     5881 cgtacccagg aaccatttac ctggtacatt gtggcggcgg cgatttacct ggtgatcacc
     5941 ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt tgagcggagg
     6001 cccagctaat gtttgagtat ttacccgaac tgatgaaagg gctacacacc agcctgacgc
     6061 taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc atcatcctga
     6121 cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta tatcacgctg tttaccggta
     6181 cgccgctgct ggtgcagatc ttcctgattt attacgggcc gggccagttt ccgactttgc
     6241 aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt
     6301 tatcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt cgtgcgatcc
     6361 cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat acgctggcga
     6421 tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg
     6481 tgttcaaaag tacctctctg gcttacacca ttacgctgat ggaagtgatg ggatacagcc
     6541 agttgttgta cggacgcacc tacgatgtaa tggtgttcgg tgcggcaggg attatttacc
     6601 tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat cgagcgcaaa gcactggcat
     6661 tcgaacggcg aaattaatgt agctgcgtac attcgccggg ggggtttgta ggtctgataa
     6721 gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg gcgtggacgc
     6781 cttatccggc ctacatagtg cataaattct tataatgaag acggacaact cactaaggta
     6841 tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat aaattcactt
     6901 gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg acagacggga
     6961 gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact ttcggtgctt
     7021 ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt gaatctatag
     7081 gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg tgcaaacaaa
     7141 tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg tcactgaaat
     7201 tcagaaaata tgacgccgta atctccggta tggatatcac gcccgagcgt agcaagcagg
     7261 tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa aaagatacct
     7321 acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc accacgcacc
     7381 agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac agttatcaga
     7441 atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac acagcggtgg
     7501 taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc
     7561 cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg
     7621 aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag aaaatcaatg
     7681 accagtggtt tccgcagtaa ttgagactga tgacaaaagt aagattgcct gatgcgctgc
     7741 gcttatccgg cccacatcaa ctctgcaatg tattgattta gcatgatttt gtaggccgga
     7801 taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg
     7861 cacctgcggc ttttttattg cttcaccagc agcgtcagca cttcatagtg cgcggtgtgc
     7921 gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc gcggatatct
     7981 ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc cattgttgag
     8041 agataatcac atagcggttt accaatgccg cggcgcggcg gattaaccag caccagctcc
     8101 ggcacctccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc
     8161 gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga tgcaatttcg
     8221 atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag
     8281 aacagatccc acatatgttt aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac
     8341 agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat ccacagcggt
     8401 acgtcattaa aacgctctgc cagcgcctgt tgttcagtaa ggtagatctc cgtctcccct
     8461 tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag ttgcggtagt
     8521 tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc agaacgcagt
     8581 acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat gtatttcagt
     8641 tcgccacgct tacgcgccac gttgtagggt gtcagccccg cacgggcgat aaacggtttt
     8701 agcgccgcaa aaacgggcgc aaatgaggca ggataaagcg ggcagtcaca aaggtcttct
     8761 ggtgtgccgt cgcgatgcag cataccgagc agtggttttt caacgctacc actcaccacc
     8821 attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca ccattcctca
     8881 accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag ttgctctgga
     8941 atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc
     9001 atacattgac cttcacatca tcaggggcga cgattataca cattattgca actggaagaa
     9061 ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg gcagcttctg
     9121 cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga acagctccgg
     9181 ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag
     9241 atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc agatctcaat
     9301 gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg
     9361 atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag
     9421 gccacggatc ataataatgg cgagcgccgc cacctgcacc agcgcaggca cattcaggcg
     9481 agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat
     9541 gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct tcagccatgt
     9601 cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag ataccaggcg
     9661 ataaatccga caattccgac cgcaccgaga attaacgttg caagggcgtt gatttccgga
     9721 ttcaccccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc tcccggcccg
     9781 gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag taaccagcca
     9841 gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt cagcggcgtc
     9901 gcaccgagat ccatcgctgc ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa
     9961 ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata
    10021 ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa caacagcgac
    10081 aagcccgtga tgacatctgg catcaccagc ggcgcggtga tcataaaggc aaagccattt
    10141 gatccgcgaa acctgccaaa gcgcaccagc accaccgccg caatagtccc gaggatcgcc
    10201 gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact catcatcgcg
    10261 tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac cgtcaccagc
    10321 ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag aaaggtaaag
    10381 cccagcagca aaatcacaat ccgccagggc gaacgaacta ccggtaaatt attcatccgt
    10441 gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca attagcagca
    10501 gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg aaaaactctt
    10561 gccatagcac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc gggatcacaa
    10621 actcgcccac agccgggata aacaccagca tcgatccggc aataatccca cctttagtca
    10681 gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc agcgctgctt
    10741 ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc ataaacggca
    10801 cgtaggcgta aacaatgcca atataaactg ccagattggt atgaagaatg gtcagcggtt
    10861 gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt tttaatattc
    10921 ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc accagtagta
    10981 ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc ggatagccga
    11041 tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga taagcatcga
    11101 aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg atggacagtt
    11161 gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc atctccgcca
    11221 ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac cagatatatg
    11281 gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac cacagcttaa
    11341 atccgcccgg cggtttcgac tgggcagcag gttcaagtgt attcatcttt gctccttaaa
    11401 ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg ccccaggtcg
    11461 gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctaatcatc tgcccacttt
    11521 tcagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc acctccccca
    11581 ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt tccgggcgca
    11641 gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact ttcagtggat
    11701 gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg agtacacctt
    11761 caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga tgctcgtaga
    11821 tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt cataatggcg atacgccccg
    11881 ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct
    11941 ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc agcgcgccca
    12001 tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc agggccacac
    12061 gctgtcgctg accaccggaa agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca
    12121 ccagcccaag catctcgttg acccgggtgg caatttccgc tttcggtagt ttgtcctgtt
    12181 tcaggccaaa ggcgatgttc tgctccacag tcatatgggg aaacagcgcg taagactgaa
    12241 acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg ccatcaagca
    12301 ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc gtggacttgc
    12361 cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc aggctgacat
    12421 catccaccgc atgttgacca tcgtaggatt tggtgaggtt gcggatttct aatagcggcg
    12481 tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg gctgctctcc
    12541 ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggcgggt gtggtgcgcc
    12601 cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca cgcgcgggtg
    12661 cgcacacggt cgattttcgg gtcctgcact ttcagagtga acagcttcgc acgaacatcc
    12721 gcaggcggat aaatacccgg gttctcacgg acttccgcac tcaccagcgg cgtggctgct
    12781 ttattggcgt tggcatagaa cacattgtcg gaaatatgcg ctactacatc cgggcgcagc
    12841 aggtaattca ggaactgata ggcttcgtct ttgtttttgg catccgcagg catggcgaat
    12901 acatcaaaga acgccatcgc cccttctttt ggaatcgaga acgagacatt cactccattc
    12961 ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac ctgcccagcc gatagcgacg
    13021 caaatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata acgaatgttc
    13081 gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatccgc tttagtgctg
    13141 ttgggatctt tgccgagata attcaacacg gtagcaaaaa cttcttctgg cgcatccagg
    13201 aaagagacac cgcagctttt cagtttttcc agattttcag gtttgaggat caagtcccag
    13261 ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt atagccaatc
    13321 ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg tttggcgacc
    13381 agcttcagca gctccgggtc gagattcttc cactccggca atttgctttt gtccagcggc
    13441 tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg cagatggaac caccaagtca
    13501 aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc gaaaacatcg
    13561 tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg ggcgatataa
    13621 tcagaccagt tataaacgtg gagtgttttt tgttcagccg cgagcgtgcc gacagagacg
    13681 gccatcagag cacccgcagc cagacccgaa agccattttt tatttaaggc ggtcatatct
    13741 ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactaaa agatatgcaa
    13801 taaacgtgca tattttttat cggcggggtg taagaaaaga gaactctctc ccggccagca
    13861 ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag actatagctc
    13921 ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg ataaagccaa
    13981 atcgacacaa gagaagtgaa ataatcttta agaaaaggtt aaaaacagca gataattttg
    14041 cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag gagttatcag tggaggaaat
    14101 agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt tgatgggcat
    14161 tagcttcaag aatgaccatc gaaatctgct cttcgctctg acgcacgaac catgcaaact
    14221 gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc tttggcagat
    14281 tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg atggcagata
    14341 aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca gaaaacagga
    14401 tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc tgcatatggg
    14461 gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg tcatcgagcc
    14521 attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg tacaacctct
    14581 ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat gatcagtaat
    14641 gtgattgcga ctaaccaccc gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc
    14701 agcggatcat tttacccgcg atgtcgatac cggtggtttt ttctattcct tccagccccg
    14761 gcgacgcatt cacctccatc accaacggcc cgcgattagc acgcagaata tccacaccag
    14821 caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga
    14881 tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg
    14941 cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg atatcgcacc
    15001 cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt
    15061 caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc
    15121 cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
    15181 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
    15241 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
    15301 tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa
    15361 tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga atagaagacg
    15421 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
    15481 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
    15541 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
    15601 cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agaataaatg
    15661 ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc cggcgattat
    15721 tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc agtgcgcctt
    15781 tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa
    15841 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
    15901 gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg atatataccc
    15961 cgcccaatcc cagcgattcc gctgcgatta atgcattctg cgccatcatt gccgtatcaa
    16021 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
    16081 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
    16141 cgccggtcag cgtcaccagt tcttcacgta acgctttgtc ggtaatgcga ataatgctac
    16201 tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacgct
    16261 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca caaataagtt
    16321 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
    16381 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
    16441 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
    16501 ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat cattccgata
    16561 aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
    16621 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
    16681 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
    16741 ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc
    16801 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgattttc
    16861 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
    16921 caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
    16981 ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct gacaagccct
    17041 cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta ctgataaata
    17101 agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat gccagctctt
    17161 gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcattgaa cagggtgccg
    17221 ccagcattgc gccaaataga ggtttcagga cttctctacg ctgtgaaaag aagctggcga
    17281 ctgctccagg aagaatgaaa aatagcaagc cgatttcagg atgcccggca gcccgaaaag
    17341 cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca aaacagcaga
    17401 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
    17461 cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
    17521 taggccaacg caattcatat agccgttgat acctaatgtg attacactag taaaatatat
    17581 tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa
    17641 acattatcgt aaattaccat ttctttcaac agcttactag taaacaagaa gttagcctcc
    17701 gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt atttgtggtc
    17761 ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact gggtaattcc
    17821 attggcgttt tagtcgtatc gctgttatta ggccaacaac atttcagcat taacaccgat
    17881 gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac
    17941 tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact ggtgatggtt
    18001 ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga tattggcctg
    18061 acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat
    18121 acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga taatctgagc
    18181 ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac
    18241 ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc ccgcgaacgt
    18301 ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc
    18361 ggcccggagc tggtggcctg gaccgacggc aaaaatctgc gtgaactggg tatttatcga
    18421 caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa tccagacggt
    18481 gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga cgcccatgcc
    18541 cgactcgatc ccagcttccg taacggtaaa gaagttttcg atcgtgacct tctcgacatg
    18601 cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ctgtaggtaa acgtctcgca
    18661 caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag ccagattgag
    18721 atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt cagcggtgat
    18781 gcccgtcgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca cagccaggtc
    18841 actgacctgc tggcattttg cgccttcttt gttattgggc tgatgatcgg gatgatcacc
    18901 ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt attcgccgga
    18961 attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc gcaaggtgca
    19021 ttaagcatgg tgaaagagtt cggcttgatg gtgtttatgg caggcgttgg tctgagcgcc
    19081 ggtagcggta ttaataacgg cctgggcgcg attggcggtc agatgttgat tgccggatta
    19141 attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg
    19201 aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc ggcaatggag
    19261 atcatcagtg atacagctcg cagtaacatc cctgcgctgg gctatgcggg cacctacgca
    19321 atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg gccaggatta
    19381 ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag aacttttccg
    19441 cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt ttgtggagcc
    19501 catcaacccc gccatttcgg ttcaaggttg atgggttttt tgttatctaa aatttatcta
    19561 ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc
    19621 ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg cggttccggg
    19681 ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca ttaccgtttt
    19741 taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca tgttatccgg
    19801 cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc cttgagcatc
    19861 actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa aactgctgaa
    19921 cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc ccaacggtac
    19981 cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc
    20041 ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc
    20101 atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt gtcttccctc
    20161 ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg aaacgccgaa
    20221 cctgggcgct gtttatgttc ttctttttgc cgggcctgtt aatggcgtcc tgggcaaccc
    20281 gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc ggtgttctct
    20341 ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta gtgaaacgct
    20401 ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg atgatgatat
    20461 taagtctggc actctggctg acatcacccc tgctcttcgc ggttggcctc ggcgtctttg
    20521 gggcaagttt tggttctgcg gaagtggcga taaacgtcga aggtgccgcc gttgagcgag
    20581 aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc acgctggcag
    20641 gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg cacattttat
    20701 tggcggcgct ggtaggaatc gcgcctattt atatcgccat tcaggcaatc cctgacggta
    20761 cgggcaaaaa tgctgccgat ggcacccagc atggtgaaaa aggcgtacct ttttatcgcg
    20821 atatccagtt gctgctgatt ggtgttgtgg tgctggcgat ggcctttgcc gaaggttctg
    20881 ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagccct acttccggct
    20941 cgctgattta tgccggtttt accctgggga tgactgttgg acgctttacc ggcggttggt
    21001 tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg ggagcgttgg
    21061 gtattgggct gattattttt gtcgatagcg cctgggtcgc tggggtgtct gttgtactct
    21121 ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc gataccggcc
    21181 ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct ttcctcgtcg
    21241 ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca atgctggttg
    21301 tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca
    21361 aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg tagacatgga
    21421 tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg ctcagtatca
    21481 gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat attatcagtt
    21541 gatctctttc ttccctgaaa ttgcgaatga aattgccttt gtggctgaaa acggcggctg
    21601 ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg cgtttgctac
    21661 tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag
    21721 cgcctataca ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa tgtattatca
    21781 ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta agtttggcct
    21841 gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg ccatcggcga
    21901 tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca
    21961 taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca gcgaagtggt
    22021 ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct ttagttttgc
    22081 aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgcgcag gctccaataa
    22141 ccgtgaaggc gtactggatg tgatcgataa agttcttaaa cacgaagcgc catttgacca
    22201 ataaaaacgg cagcacgccc ggcaacacaa tgcctgaatg aaggctgcat aattcgcgga
    22261 aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg
    22321 cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc gatgtgccga
    22381 gtggatacaa agtggttgtg gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg
    22441 cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac cgcccgcatc
    22501 cttatgttcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac cagattgacg
    22561 accatcacca ttaattcgag aatgcctgat cgcacaaagc aatcaggcat ttttttaccc
    22621 ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg acagccacaa
    22681 aattccgttg acaaggttga agagattaaa cagtccgttg cccccgttca gccaggcatg
    22741 tttgctgatt tcaataccga cggtaaagat cagcatttgc agcattccca tcgccgcaga
    22801 aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc
    22861 cagaccaatg ccgaaagcat aaatacttaa cccggcggtc atccataaat acgcgtgcga
    22921 tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc agccgcccat
    22981 aattatcagc gaacgtacgg tgcggcgcga ggtcaaacgc gctaacaaca agttacccgc
    23041 aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc tcaactgctc
    23101 gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca ggctaacgaa
    23161 tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct tatagtcacg
    23221 accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag gcatggcttg
    23281 ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aagacaaaca tcccttccca
    23341 gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag caatcagcgc
    23401 cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg attcctgaat
    23461 tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga agcgcaacag
    23521 agtgaattgt tcaatgtttt gcgccagtaa tattgccaga caggtgatga taaaccacac
    23581 cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc ccaggagcca
    23641 ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc aatcaatgcc
    23701 cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt tgccgatata
    23761 ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac gtccaagcct
    23821 ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat cgcgcagagt
    23881 ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg gttaagcaca
    23941 ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac ctatgcccgt
    24001 tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg ctttacccag
    24061 tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta atcactttgc
    24121 gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt aacgcgacgc
    24181 cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat ttgattaccg
    24241 tggtaccgct gctggccgcg gtgctttggt tgtgggggct tacagcacaa cggcaactgg
    24301 tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg atgggacatc
    24361 tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg catcatgcgg
    24421 cggatgactc attcccaagc gaccacggta cggtgatttt cacctttgca ctggcatttt
    24481 tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc gtcattgcct
    24541 ggtcgcgcgt ttatcttggc gtgcactggc cgctggatat gctcggtgga ttgctggctg
    24601 gtatgattgg ctgccttagc gctcagatta tctggcaagc gatggggcat aaactctatc
    24661 aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa ggctgggtgc
    24721 gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa ctattcagag
    24781 ggattatgga aacacgtcgc gaagagcgca tcgggcagct gctgcaagaa ttaaaacgca
    24841 gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag atgacgattc
    24901 gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat attgttctgg
    24961 aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg gtggaagaaa
    25021 aacgccgggc ggcaaaaatg gctgcgacac tggtagaacc cgatcagact ctcttttttg
    25081 actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc ccttttaccg
    25141 ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat tgccgcgcat
    25201 ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaacccatc gattttcagc
    25261 aaacgttgaa taatttttgc ccggatatcg ctttttattc cgcggcgggc gtgcatgtca
    25321 gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg gccatgtcga
    25381 tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg cgtccggcgc
    25441 gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg gaagatgagt
    25501 atgtgaagta cgcgcagacg caccgtatta agttgatgta ttaatgacgt ataaccggat
    25561 gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga accactgatg
    25621 gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt ccacattttc
    25681 catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat caatggtccc
    25741 gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg tgtagctcgc
    25801 cttcaggttt ttcagctgcc cacgcggtat ggtcactgag cccgcttcgc ctgccccaag
    25861 attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg catcaggttt
    25921 aattggcgtc acggtttcaa agaagcggaa accccaggtc aataatttct cagactcatt
    25981 aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca tatcgccctg
    26041 ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg gttcctgtct tcatgccatc
    26101 aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa ttttgttgaa
    26161 ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga tcaatgcttt
    26221 acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca gaccgtgcac
    26281 cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca tcaaaccaat
    26341 gaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt cattaccgga
    26401 ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa
    26461 cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt taccgaccgt
    26521 caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca catagctggt
    26581 catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt ctgccagcac
    26641 tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac
    26701 ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc
    26761 aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat ccattcaaaa
    26821 aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac acaaacttaa
    26881 agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc ttcgctgctt
    26941 ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac catcggagtc
    27001 ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg
    27061 aataattcaa ccaacgtaaa aaaagtattg ctgacgctcg aagaactgga actaccttat
    27121 gagcaaattc tcgcgggccg tgagtttggg ataaatcacg atgctgattt tctggcgatg
    27181 aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa
    27241 tcaaacgcca ttgtccgcta tctggcggca cagtacgggc aaaaacgtct gtggatcgac
    27301 tcaccggcac gtcgtgcgga agcggaaaaa tggatggact gggcaaacca gacgctcagt
    27361 aatgctcatc gcgggatcct gatgggatta gtcagaacac caccggaaga gcgcgatcag
    27421 gccgccattg atgccagttg caaagagtgc gacgccctgt ttgccctgct cgatgcggaa
    27481 ctggcaaaag taaaatggtt ctctggcgac gagtttggtg tgggcgatat cgctatcgca
    27541 ccgttcatct acaatttgtt taacgttggc ctgacctgga caccgcgtcc aaatctgcaa
    27601 cgctggtatc agcaactcac tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt
    27661 agctaattgc gtgggctaac tttaagtaat tccccactgg actcgtcggt gagaacgtat
    27721 aaataaccgt cgggtccagt gcgaacatca cgaattcgct gccctctgtc cgttaaaata
    27781 cggccatctt ctgtcacttt gtcgccgttg acgctcatca caatgacatc tttatctttc
    27841 agcgcgccaa taaataattt ttgctgccac tgggggaatt tatcgctgtt atagaaggcc
    27901 atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt cccggcgacg
    27961 atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca ggttgccagc
    28021 ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc gcgcgggcca
    28081 tgttcattca gccacagtgc attactccac ggattcattg ccattccttg cggattacga
    28141 atgccataag accagatctc ggcgcgcaca ccggattcct ttataaaagg attatcatcc
    28201 gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc cagatcctgc
    28261 gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt accgtcgaat
    28321 accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg gaagacggtg
    28381 cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac agcagttccg
    28441 gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa
    28501 tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac gtccggaact
    28561 ccggaaagcg gcgcagataa tccttttcct gcttgccagt gacgcaactc gccgccgcgc
    28621 agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg atggtcgagt
    28681 ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc ggaagaaaga
    28741 caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctccgga ttgactctgg
    28801 cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact taaacatgaa
    28861 gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg tgcttctgca
    28921 ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg gcgttgacgg
    28981 taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct gtcgctaatg
    29041 cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga ttcaaccgca
    29101 gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga tgattgcttt
    29161 cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc
    29221 gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac atacttcgat
    29281 cctcctcttt ccagccttgt tgcaattaaa tagtatggct attttttgag caaaatgctg
    29341 tccaggatca attcttcacg ccgaattaac aaaatttaca tttccttcgc ctccgccagc
    29401 gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc ctgacctttc
    29461 ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa aatcggcttt
    29521 gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac cgaactacgc
    29581 actgaaggtt atgacgtggt accgagctat gacgatgcgg acatggtgat cgtcaacacc
    29641 tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga agcgttgaat
    29701 gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca gatccgcgaa
    29761 gtccacccga aagtgctgga aatcaccggg cctcatagct atgagcaggt tctggagcac
    29821 gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt gccagaacaa
    29881 ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac
    29941 cgctgcacct tctgcattat tccgtctatg cgcggcgacc tggtgagccg tccgattggc
    30001 gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat tctggtgatc
    30061 tcgcaggata cttctgccta tggcgttgat gttaaacatc gtactggctt ccacaacggc
    30121 gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg
    30181 acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc actgatggca
    30241 gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc gcgcattctc
    30301 aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc
    30361 gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc tggcgagaca
    30421 gaagaagatt tccagatgct actcgacttc ctgaaagaag cgcgtctgga tcgcgttggc
    30481 tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga ccaggttccg
    30541 gaagaagtta aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc
    30601 gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga agtggacgaa
    30661 gaaggcgcga ttggtcgcag catggcagat gcaccggaaa tcgacggcgc ggtttatctc
    30721 aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat
    30781 gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc
    30841 ggcctgcgaa taatgattac tgccagctaa tctgagccct gccgtttttc ttactcttat
    30901 acagccgctc atccgccagc attttcaagc gttcgacggt ggtacattca tttgaatgcg
    30961 ctacaccgat actggtactt aaattaatgc ttcccacctg gaaatgaacg atttctttta
    31021 gaatcgatag caactcttcc agcgttccac ccgtataaat caccgcaaat tcgtcaccgc
    31081 cgaagcggta gattttacct ttatctaccg ggatctgtga tttaaggatt ttcaccactt
    31141 tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat
    31201 cgacgtcaaa caggcacaaa taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa
    31261 aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta tacatactgt
    31321 agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat
    31381 ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg cgtaccagat
    31441 agaggcaact ggtacccgat atcactgcca ccaatgcggc aaagaaaata atcaatgtac
    31501 agacccgcaa ataaacatct tttttatacg caaatacgta caatccggtc ggcaaatagg
    31561 cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcataa tattcatcac
    31621 cggtcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg tccatcgcca
    31681 gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt
    31741 gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat
    31801 ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact
    31861 cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag
    31921 agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt tcctccgtgg
    31981 gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca
    32041 ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt
    32101 gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa
    32161 gtacgaactt attgattctg gacatacgta aactactctt ttactaattt tccactttta
    32221 tcccaggcgg agaatggcat gggtttctgg tacagatacc cctgagcctg aacacatcct
    32281 aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc
    32341 cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata
    32401 tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc
    32461 aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt
    32521 tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca
    32581 acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga
    32641 aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc
    32701 agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa
    32761 cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg
    32821 atcccccgca ccgcagaaac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg
    32881 ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga
    32941 atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc atcacagaaa
    33001 ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg
    33061 taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga
    33121 ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc
    33181 tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag
    33241 cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt attgcgttca
    33301 tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc
    33361 agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc gctttcctga
    33421 tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc
    33481 gcgatcgaat ttttgatgct gataatctct ttcgcatcgc cttcgcgaat tttcagagcc
    33541 tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat taaccagctc ggtcatttca
    33601 gaaaattgcc gacggaacac acgagtggtc atgaaatata ccaccagcaa cacgataata
    33661 atgaacgcaa acggtagata aaaaagttta tctgatgcgt tcaataaatt accgccagga
    33721 taataaaaat agatttcgaa caatgcatta tttggcactg ctgatgtctt tgcaatatac
    33781 tccgtatcga cagaatcaat aatattataa ttaccttcgc tattataaat aatcagtttt
    33841 tcacgagcga gttgattctt ggcaatgatc tcatgacctt gtttcaaaaa tagaacttca
    33901 tcattagcgc tgatttgatc aagatactcg ttcagatacc gccagtttaa atcgacatga
    33961 attttatcta ccgctaacaa atcgtgatct ttgccgttga tacgtagcgt aaaagagata
    34021 cattttttac ccggacgatc aaagcaattg tatgccggat tccagctgtt gttagagtca
    34081 tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat aacccgccct
    34141 gactcgtcga caagcggtcc atttacttgt tcctgatgat aaacaacgat tttatcattc
    34201 gccctccgtg aaaagagata atatttatta tcatcaagta ccagtcccac cgagtctaaa
    34261 aatggcatca tttccatttt cagaatcacg gcgcgttgaa tctcctctgt attttttaat
    34321 aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca
    34381 acgtttttct gaataagttc aaagcggc
//
LOCUS       DAZFDP010000032        33936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFDP010000032 DAZFDP010000000
VERSION     DAZFDP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(132..884)
                     /gene="tcyN"
                     /locus_tag="ACHJ1G_001708"
     CDS             complement(132..884)
                     /gene="tcyN"
                     /locus_tag="ACHJ1G_001708"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4763580.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="ACHJ1G_001709"
     CDS             complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="ACHJ1G_001709"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4763581.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="ACHJ1G_001710"
     CDS             complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="ACHJ1G_001710"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4763582.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="ACHJ1G_001711"
     CDS             complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="ACHJ1G_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4763583.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="ACHJ1G_001712"
     CDS             complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="ACHJ1G_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4763584.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="ACHJ1G_001713"
     CDS             complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="ACHJ1G_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4763585.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5024..6487)
                     /locus_tag="ACHJ1G_001714"
     CDS             complement(5024..6487)
                     /locus_tag="ACHJ1G_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO4763586.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6742..8154
                     /gene="fliD"
                     /locus_tag="ACHJ1G_001715"
     CDS             6742..8154
                     /gene="fliD"
                     /locus_tag="ACHJ1G_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4763587.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8169..8579
                     /gene="fliS"
                     /locus_tag="ACHJ1G_001716"
     CDS             8169..8579
                     /gene="fliS"
                     /locus_tag="ACHJ1G_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4763588.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8579..8944
                     /gene="fliT"
                     /locus_tag="ACHJ1G_001717"
     CDS             8579..8944
                     /gene="fliT"
                     /locus_tag="ACHJ1G_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4763589.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9022..10509
                     /gene="amyA"
                     /locus_tag="ACHJ1G_001718"
     CDS             9022..10509
                     /gene="amyA"
                     /locus_tag="ACHJ1G_001718"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4763590.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10543..10956)
                     /gene="yedD"
                     /locus_tag="ACHJ1G_001719"
     CDS             complement(10543..10956)
                     /gene="yedD"
                     /locus_tag="ACHJ1G_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4763591.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11143..12348
                     /gene="yedE"
                     /locus_tag="ACHJ1G_001720"
     CDS             11143..12348
                     /gene="yedE"
                     /locus_tag="ACHJ1G_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4763592.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12345..12578
                     /gene="yedF"
                     /locus_tag="ACHJ1G_001721"
     CDS             12345..12578
                     /gene="yedF"
                     /locus_tag="ACHJ1G_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4763593.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12685..13353
                     /gene="yedK"
                     /locus_tag="ACHJ1G_001722"
     CDS             12685..13353
                     /gene="yedK"
                     /locus_tag="ACHJ1G_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4763594.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(13748..14062)
                     /gene="fliE"
                     /locus_tag="ACHJ1G_001723"
     CDS             complement(13748..14062)
                     /gene="fliE"
                     /locus_tag="ACHJ1G_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4763595.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14277..15935
                     /gene="fliF"
                     /locus_tag="ACHJ1G_001724"
     CDS             14277..15935
                     /gene="fliF"
                     /locus_tag="ACHJ1G_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4763596.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15928..16923
                     /gene="fliG"
                     /locus_tag="ACHJ1G_001725"
     CDS             15928..16923
                     /gene="fliG"
                     /locus_tag="ACHJ1G_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4763597.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16916..17602
                     /gene="fliH"
                     /locus_tag="ACHJ1G_001726"
     CDS             16916..17602
                     /gene="fliH"
                     /locus_tag="ACHJ1G_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4763598.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17602..18975
                     /gene="fliI"
                     /locus_tag="ACHJ1G_001727"
     CDS             17602..18975
                     /gene="fliI"
                     /locus_tag="ACHJ1G_001727"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4763599.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            18994..19437
                     /gene="fliJ"
                     /locus_tag="ACHJ1G_001728"
     CDS             18994..19437
                     /gene="fliJ"
                     /locus_tag="ACHJ1G_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4763600.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19434..20561
                     /gene="fliK"
                     /locus_tag="ACHJ1G_001729"
     CDS             19434..20561
                     /gene="fliK"
                     /locus_tag="ACHJ1G_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4763601.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            20666..21130
                     /gene="fliL"
                     /locus_tag="ACHJ1G_001730"
     CDS             20666..21130
                     /gene="fliL"
                     /locus_tag="ACHJ1G_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4763602.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21135..22139
                     /gene="fliM"
                     /locus_tag="ACHJ1G_001731"
     CDS             21135..22139
                     /gene="fliM"
                     /locus_tag="ACHJ1G_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4763603.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22136..22549
                     /gene="fliN"
                     /locus_tag="ACHJ1G_001732"
     CDS             22136..22549
                     /gene="fliN"
                     /locus_tag="ACHJ1G_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4763604.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22552..22917
                     /gene="fliO"
                     /locus_tag="ACHJ1G_001733"
     CDS             22552..22917
                     /gene="fliO"
                     /locus_tag="ACHJ1G_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4763605.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22917..23654
                     /gene="fliP"
                     /locus_tag="ACHJ1G_001734"
     CDS             22917..23654
                     /gene="fliP"
                     /locus_tag="ACHJ1G_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4763606.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23664..23933
                     /gene="fliQ"
                     /locus_tag="ACHJ1G_001735"
     CDS             23664..23933
                     /gene="fliQ"
                     /locus_tag="ACHJ1G_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4763607.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23942..24727
                     /gene="fliR"
                     /locus_tag="ACHJ1G_001736"
     CDS             23942..24727
                     /gene="fliR"
                     /locus_tag="ACHJ1G_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4763608.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25017..25640
                     /gene="rcsA"
                     /locus_tag="ACHJ1G_001737"
     CDS             25017..25640
                     /gene="rcsA"
                     /locus_tag="ACHJ1G_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4763609.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25684..25872)
                     /gene="dsrB"
                     /locus_tag="ACHJ1G_001738"
     CDS             complement(25684..25872)
                     /gene="dsrB"
                     /locus_tag="ACHJ1G_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4763610.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26035..26262
                     /gene="yodD"
                     /locus_tag="ACHJ1G_001739"
     CDS             26035..26262
                     /gene="yodD"
                     /locus_tag="ACHJ1G_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4763611.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26560..27375
                     /gene="yedP"
                     /locus_tag="ACHJ1G_001740"
     CDS             26560..27375
                     /gene="yedP"
                     /locus_tag="ACHJ1G_001740"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4763612.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(27372..29066)
                     /gene="dgcQ"
                     /locus_tag="ACHJ1G_001741"
     CDS             complement(27372..29066)
                     /gene="dgcQ"
                     /locus_tag="ACHJ1G_001741"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO4763613.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(29304..29486)
                     /gene="yodC"
                     /locus_tag="ACHJ1G_001742"
     CDS             complement(29304..29486)
                     /gene="yodC"
                     /locus_tag="ACHJ1G_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4763614.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29565..30482)
                     /gene="yedI"
                     /locus_tag="ACHJ1G_001743"
     CDS             complement(29565..30482)
                     /gene="yedI"
                     /locus_tag="ACHJ1G_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4763615.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            30655..31575
                     /gene="yedA"
                     /locus_tag="ACHJ1G_001744"
     CDS             30655..31575
                     /gene="yedA"
                     /locus_tag="ACHJ1G_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4763616.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(31564..32034)
                     /gene="vsr"
                     /locus_tag="ACHJ1G_001745"
     CDS             complement(31564..32034)
                     /gene="vsr"
                     /locus_tag="ACHJ1G_001745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4763617.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(32015..33433)
                     /gene="dcm"
                     /locus_tag="ACHJ1G_001746"
     CDS             complement(32015..33433)
                     /gene="dcm"
                     /locus_tag="ACHJ1G_001746"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4763618.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33500..>33936)
                     /locus_tag="ACHJ1G_001747"
     CDS             complement(33500..>33936)
                     /locus_tag="ACHJ1G_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO4763619.1"
                     /translation="EFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEA
                     LGAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTAR
                     GKQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         8283 a         8806 c         8607 g         8240 t
ORIGIN      
        1 tgaattactt ttgcaagcca tcacattctc ttatgttatt aatgagttat gctgatttgt
       61 taagcagttt tatcacgctt gaaatagcgt cccgccccga caggtgaatc gtcggggctg
      121 attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctgg
      181 ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa
      241 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg
      301 cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc
      361 agcgccgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgtgca
      421 atcgcaacac gttgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
      481 gccagcccaa ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
      541 cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa
      601 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
      661 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc
      721 ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg
      781 ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga
      841 ccgtgaaatt ttttcaccaa gttcttaact tcaatggcac tcattttggt tctctctcct
      901 ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc
      961 agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca
     1021 actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatccttta
     1081 ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     1141 tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac
     1201 ctttatcaat agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca
     1261 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
     1321 cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa
     1381 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     1441 cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
     1501 tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
     1561 gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag
     1621 aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc ccgccatcgc
     1681 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
     1741 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtat
     1801 aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
     1861 tttcggcaac tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
     1921 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac
     1981 taccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
     2041 cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa
     2101 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
     2161 ttcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa
     2221 atagttttct gcggttgtgc caataggatt ttccagcagc gccacacagt gcagaccgag
     2281 tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgccc cggcagtaat
     2341 cagcgtatcg gcaccttcgc gcagagcatc tgctgcgaga aattccagct tacgtaattt
     2401 attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtccaag
     2461 ataatcagaa aagcgcggca gatattcgag cggtgttggc gcgccgataa actccaaccg
     2521 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
     2581 tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctggtgaaag cgcccttttt
     2641 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc
     2701 agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag caggtcttca
     2761 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
     2821 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca
     2881 tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
     2941 tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc
     3001 acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc
     3061 agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt cttgcgctca
     3121 tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc
     3181 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
     3241 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
     3301 ggataagtgc cttccagccc taccagcagc gtgccgcgct ctttaacttt attaagcaga
     3361 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgctacggc catcacactc
     3421 atcaatgcct gacgtcccag atgtgctaat ttcatattta ccccgaatgt tgttatgtct
     3481 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
     3541 atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacacgtt
     3601 tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt gctggtggtg
     3661 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagcggatc gagggaaata
     3721 ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta
     3781 cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
     3841 tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgatttt
     3901 tgttccgttt gccagccatt ttcatcaagc agcgcgtcca ggcgggaaat ctcgatttta
     3961 ttcactattt tgccgtcgcg aacgagggta atgcgatcga gtaatttacg gcaatgcgcg
     4021 caatgagtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
     4081 tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt
     4141 tataacttac ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa ctgactgacc
     4201 cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa
     4261 tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc
     4321 tgacgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta
     4381 accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
     4441 ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct
     4501 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
     4561 acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
     4621 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg
     4681 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
     4741 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
     4801 taacgctgcc atagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
     4861 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
     4921 atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa attgcgggca
     4981 gaaaaaaccc cgccggtggc gggggttgag cgataagtgt aaattaacgc agcagagaca
     5041 gtacgttctg cgtggtctgg ttagcctgcg ccagaacaga ggtacccgct tgttgcagga
     5101 tctgcgcacg agacatgtta gacacttcgg tcgcgtagtc agcatcttcg atacggctac
     5161 gggcagaaga caggttgttt acggtgttgc caaggttggt gatagcagag tcgaaacggt
     5221 tttgtactgc accgaggtca gaacgcagat tgtcaacttt agccaatgct ttgtcgatag
     5281 tttcgagcgg gttggtggta gattgcaacg attttgctgc atcttcgttt accagaatcg
     5341 ggctaccacc ttctgatttg ctcagataca tgactttatt gttaccagaa gcagtctccg
     5401 ttatcgtttt accatctgca ctaacatcgt aagttgcacc gttaacaact aacgtgcttc
     5461 ctgttttctt agctgcattg agatcaagat cggaaagtgt ggcagccttg ttatcaactt
     5521 tggttgtcgt ataaaggcca gcgctatttt tgtaaactgt ctcactcgta atcaaagaag
     5581 cttttttgtc agcatctgtc acagtaaatg tcactgactt accatcaatt tctgcagaat
     5641 atacaccatt cccattgcca tccgctgcag ttgttgttgt gaattcaact ccattaaacg
     5701 tgaatttatc attctgtgct ttattcttag ccgctgctgc gagtgctgta gcatcaatcc
     5761 ctgtaccagc tatgtttgtg tcagatttag ttgtcagtga accatctgct gcactaacaa
     5821 aaatatcatt ccctgtagta tctttaacag ctccgctatc tacgttaaca gtataagcat
     5881 caccgccaac atcatagtta tcagtacctg tcgctttaaa tttagaaatc aggtcactgc
     5941 cagttgcttt ctgcgcgcca tcgatattaa aaccgtccag gccgagagtt ttcgcatcaa
     6001 tttttgccag attgatagtg atggtttcac catcattagc accaacctga attttcattt
     6061 cattattttc agcaaggact ttcacgccgt taaactgagt ttgctcagat acacggtcaa
     6121 tttcttccag acgttgagta atttcagcct ggatagaaga aagatcgctg tcagagttag
     6181 taccgttagt tgcctgaaca gtcagttcac gtacacgctg caggttgttg ttaatttcat
     6241 tcagcgcacc ttcagtggtc tgcgcaacag aaataccatc attcgcgtta cgggaagcct
     6301 gagtcagacc tttaatattt gctgtaaaac ggttagcaat cgcctgacct gctgcgtcat
     6361 ctttagcact gttaatacgc aggccagaag agagacgttc aatggcggag ctcagagaag
     6421 actgagattt gttcaggtta ttctgggtca acagcgacag gctgtttgta ttaatgactt
     6481 gtgccatgat cttttcctta tcaattacaa cttgatgtta ttgggctgtt gcccacggtt
     6541 tctcaccgta ccccttgtat cggcacctga atttcgaact ttagaaaatt ttagtttctc
     6601 tcctgttttt tcttaacggc tgaaatatcc gtaatataca actctactct gcgcatcatc
     6661 ttcgtgaatc aactattaaa ctttgcgcac aaattgccga taacccgctt atctactgtt
     6721 tgcaatcaaa aggaattagg tatggcttca tttacatctc ttggcgttgg ttcgaatctg
     6781 ccgctggata cattgctaaa caacctgacc atcgctgaaa aaaaacgctt aaacccaatt
     6841 actcagcaac agagtgacaa taccgcacgt ctgacagcct atggcacatt aaaaagtgca
     6901 ttagaaaaat tccagactgc aaatactgcg ttaaataaag ccgatctgtt caaaagcact
     6961 accgttacca gtagcaatga agaccttaaa gtttccaccg aagcaggtgc tgcccccggt
     7021 atctatacca tcagcgtaac ccaactggcg caggcgcaat ctttacgaac tgattccccg
     7081 acaatcattg catcaacaaa agatgcactg ggtgatgagt catcagatac acgcacaata
     7141 aaaattaccc aagacggtcg taaagagccg ctggaaatca agctcaataa agatcagacg
     7201 tcattggatg agatcagcaa agccattaac gatgccgata gtggaatttc cgctagtatc
     7261 gttaaagtca aagatggtaa ctatcaattg gtattgaccg ccagtgaagg tctggccaat
     7321 aaaatgacca tatctgttga gggtgacagt aaattaaatg atctgctggc ttatgacagc
     7381 aagaccaaca ccgggaatat gaaggaactg gttaacgcgc aaaatgcaca gctaaacgtt
     7441 aacggtatcg atattgaacg ttcaagtaac aaaattaccg atgctcctca gggagtgact
     7501 ctcgatttga ccaaaaaagt cactgacgtc cgggtcacgg tgacaaaaag caacgataaa
     7561 gcaaccgaag caattaaagg ctgggtggat tcatataact ccctcattga cacctttaac
     7621 accctgacaa aatataaaga agtagaccca ggtgctgaag cacaagataa aaataatggt
     7681 gcacttctcg gagacagcgt tgtacgtaca attcaaagcg gcatccgcgc acaatttgca
     7741 aacggtgcca gcgacagtgc cttcaaaaca ttaaacgaga tcgggattaa gcaggacggc
     7801 acgacgggta agctgaaaat tgatgatgat aaactcaaaa aagtcctgaa tgaaaataca
     7861 gcatccgtac gtgaattgct ggtaggcgat ggtaaagaaa caggaattac caccaaaatt
     7921 gccaccgagg taaaaggtta ccttgccgat gatggcatta ttgatagcgc acaggacagt
     7981 attaacgcca cactgaaaaa actgaccaaa cagtatctat ccgtcagtgc cagcattgat
     8041 gacaccgtgg cgcgttacac tgcacagttt acgcaattag ataccatgat gagcaaactg
     8101 aataacacca gtacctatct cagccaacaa tttactgcaa tgagtaattc ctgatcacgg
     8161 aggtcagcat gtataccgcg agcggctcaa aagcctatgc acaaattggc gtcgaaagcg
     8221 ccgtaatgag cgccagccag cagcagctgg tcaccatgct atttgatgga gtgctgagcg
     8281 cactggttag agcacgcctg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt
     8341 tgtcaaaagc gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca
     8401 aagacgaatt aacccaaaac ttgattgctc tttatagcta tatggtcagg cgcttgctgc
     8461 aagccaattt acgcaacgat gtctccgcag tcgaagaagt ggaagcatta atgcgcaata
     8521 ttgccgatgc ctggaaagag tcgttactct ccccttcttt gattcaggac ccagtctgat
     8581 gaaccacgca ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat
     8641 gttacgcctg gcaacagaag aacaatggga cgaactcatc gccagcgaga tggcgtatgt
     8701 gaatgcggtg caggagattg cacatctgac tgaagagatt gacccgtcca ccacgatgca
     8761 ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg taaagcagtt
     8821 attacagatt cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc
     8881 ggtgttaagt gcctatggtg atcagggcgg ctttgtgctg gctccgcagg ataacctctt
     8941 ttgaatctga acgagtcgat ggctcgcgaa taatccgatt acggctacgc ttctaatgtt
     9001 ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat
     9061 tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg ttttaatgat
     9121 attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg cgggtattcg
     9181 gtcggctacg actcctatga tttatttgat ttaggcgagt ttgatcagaa aggcagcatc
     9241 cctactaaat atggcgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat
     9301 gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa
     9361 gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt
     9421 gagtgtgaag gctggacgcg ttacaccttc cccgcccgtg ccgggcaata ctcgcagttt
     9481 atctgggatt tcaaatgttt tagcggtatc gaccatatcg aaaaccctga tgaagatggc
     9541 atatttaaaa ttgttaacga ctacaccggc gaaggctgga acgatcaggt tgatgatgaa
     9601 ttaggtaatt tcgattatct gatgggcgag aatatcgatt ttcgcaatca tgccgtgacg
     9661 gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggttttcgt
     9721 cttgatgcgg tcaaacatat tccggcgtgg ttttataaag agtggatcga acacgtacag
     9781 gaagttgcgc caaagccgct gtttattgtg gcagagtact ggtcgcatga agtcgataaa
     9841 ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc tgttcgatgc gccgctgcag
     9901 atgaaattcc atgaagcatc gcgcatgggg cgcgactacg acatgacgca gattttcacg
     9961 ggtacattag tggaagccga tcctttccac gccgtgacgc tcgttgccaa tcacgacacc
    10021 caaccattgc aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgct
    10081 ctaattttgt tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgcg
    10141 cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa
    10201 cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacgttattt
    10261 ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ctgacgaata tcccggctgc
    10321 gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg agagaattac
    10381 ggcaataaaa cctggcgtga ttttctgggg aatcggcaag agagcgtagt gaccgacgaa
    10441 aacggcgaag cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag
    10501 gtgatttaaa ttcatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg
    10561 taacggcgct ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat
    10621 cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt
    10681 tacggtaacg ttagtgagat catcagacat caatgtcaac ttgcccggca cggcaatcac
    10741 ccgctgccac tgacggcaat ccagggtatc gccctcttta gtgacaatca ggctgcctat
    10801 cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgctagcca
    10861 gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag
    10921 caacatgagc gcgcttgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa
    10981 gaggattgtg gcattaaagc cctgatgtcg ccagcctttc caggttcaga gagaaatttg
    11041 atctaactat tatttttgtg aattattacc ccgaaaataa atttgcatga tgtatgattc
    11101 gcgattcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc
    11161 aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt
    11221 ctctccactt actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc
    11281 cgttggggcg gccagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt
    11341 aaaattatcc atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggc
    11401 atgtttggcg gctgctttgc cgcagcgctg tgggccaaca atgtcaaact gcgcatgcca
    11461 cgcagccgta tccgcattat gcaggccatt attggcggta ttatcgctgg ttttggcgcg
    11521 cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gtattcctca gttctcgctg
    11581 catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc acgctttacc
    11641 ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg
    11701 acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggt
    11761 atgctgggct gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg
    11821 tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc
    11881 cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg
    11941 gtgagtgcca tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg
    12001 gcgggaccaa acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggcaggc
    12061 ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc
    12121 ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt actgggatga tttcgctccg
    12181 gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cctttggccc gatgggtggc
    12241 ctgctggtga catatttgct gttgtttact gcgctgatgt tgattatcgg ctgggaaaaa
    12301 cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc
    12361 ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaactcttg
    12421 aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccacagt
    12481 cgatcaataa tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc
    12541 aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc
    12601 acagcgggaa catcgttgcc tctgacacat agattttacc cgccccctct cctattcttt
    12661 tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa
    12721 gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc
    12781 attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa
    12841 caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg
    12901 ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc gctctggcaa
    12961 catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa
    13021 aaacagcctt attttatcta tcgcgctgat ggacaacctg tttttatggc cgcgataggt
    13081 agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac tgcagcagca
    13141 gaccagggtc tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc agaagctgca
    13201 caggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc taccaacggc
    13261 tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtggg taatatcaaa
    13321 aaccagggag cggagttaat tcaaccggtt tgataagcca ggagactatt gatcaataat
    13381 actgcgtcat aagaaatctc tattagacaa tagtagacgc tccttccaca tgcgccacaa
    13441 gaaacctcta ttccagtgac acaattacgc ctgattattt ccctagcccg gaatatgaat
    13501 aaaaacatca acaaagatta aattattcaa acgttactgt tgcgtctgcg gtcgtagcaa
    13561 catccgacgt ggtgatgagt gttaaaagag acactctgat gttcagaggg gttatcgcgt
    13621 ttacggatcg tggatggcag aaaactttca ggatcaggta tccattttaa accagaaatt
    13681 gagtgagttt gccccatcca tgccccaagc ggtgagattg gaagtaatta acaacaggtt
    13741 acaaaaccta cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca
    13801 cctgaatccc catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta
    13861 acgccacgcc gggttcaccg agggtgaatt tttctgcctg cgtgcgggca gctgtttgtg
    13921 tatcgctaat gcgatcgagc gtggcgtgca gctgcccggc aaaactaatg gtcggttgcg
    13981 gcagtgaatc ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt
    14041 caatcccctg tatcgctgac attttcgtct cctggataat ttctggtagc aaagcctacc
    14101 agtaagtcaa taagataaag gcgctaaata acaacaaaaa aacgggttta ttggcggata
    14161 gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt cgagtttgct gacccgggag
    14221 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga
    14281 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
    14341 aaattccatt gattgttgcc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
    14401 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
    14461 caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg
    14521 ctattgaagt tccggcagat aaagttcacg aactgcgtct gcgcctggca caacaaggtt
    14581 tgcccaaagg cggcgcggtc ggtttcgaac tactcgatca ggaaaagttt ggtatcagcc
    14641 agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg
    14701 aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat
    14761 tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg
    14821 cactcgatga agggcaaatt agcgccattg tgcatctggt ttccagcgcc gttgctggtc
    14881 tgccgccggg aaacgtcacg ctggtggatc agggcggaca tctgttaacc cagtccaata
    14941 ccagcgggcg cgatcttaat gatgctcagt tgaaatatgc cagcgatgtc gaaggccgta
    15001 ttcagcggcg tattgaagca atcctgtcgc ctattgttgg taacggtaat attcacgccc
    15061 aggtcacggc gcagctggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta
    15121 acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg
    15181 gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata
    15241 acgcgccaat cagcacgcct ccggcaaatc aaaataaccg ccagcagcag gcgagcacca
    15301 ccagcaatag cgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc
    15361 gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag
    15421 tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga
    15481 agcaaattga agatctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc
    15541 tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag cggcggagaa ctgccgttct
    15601 ggcaacagca agcgtttatc gatcagttgc tggctgccgg tcgctggttg ctggtactgc
    15661 tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca gttaacacgt cgcgccgagg
    15721 cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga agtggaagat gcggtggaag
    15781 tccgcctgag caaagacgaa caactccaac aacgacgcgc taaccaacgt ctgggggcag
    15841 aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg
    15901 tcattcgcca gtggataaat aacgatcatg agtaacctga caggcaccga taaaagcgtc
    15961 atcctgctga tgaccattgg cgaagaccgg gcggcagagg tgttcaagca cctctcccag
    16021 cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc caacaagcag
    16081 ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac agtttgccgc actgaatatc
    16141 aacgccaacg attatctgcg ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc
    16201 agcctgctgg aagatattct cgaaactcgc gataccgcca gcggtattga aacgctcaac
    16261 tttatggagc cgcagagtgc cgccgatctg attcgcgatg agcatccgca aattatcgcc
    16321 accattctgg tgcatctgaa gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa
    16381 cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg gcggcgtgca gccagccgcg
    16441 ctggcggaac tgaccgaagt actgaatggc ttgctcgacg gtcagaatct caagcgcagc
    16501 aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa
    16561 gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac
    16621 gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc gcagcattca gcgtctgttg
    16681 caggaagtgg attccgaatc gctgttgatc gcactgaaag gggccgagca gccactgcgc
    16741 gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc
    16801 aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt
    16861 gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca gcggcgagga tacctatgtc
    16921 tgataatctg ccgtggaaaa cctggacgcc ggacgatctc gcgccaccac aggcagagtt
    16981 tgtgcccatg gtcgagccgg aagaaaccat cattgaagag gccgaaccca gccttgagca
    17041 gcaactggcg caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg gtattgccga
    17101 aggtcgccag caaggtcata agcagggcta tcaggaagga ctggcccagg ggctggagca
    17161 aggtctggca gaggcgaagt ctcaacaagc gccaattcat gcccggatgc agcaactggt
    17221 cagcgaattt caaactaccc ttgatgcact tgatagtgtg attgcttcgc gcctgatgca
    17281 gatggcgctg gaggcggcac gtcaggtcat cggtcagacg ccaacagtgg ataactcggc
    17341 actgatcaaa cagatccaac agttgttgca gcaagaaccg ttattcagcg gcaaaccgca
    17401 actacgcgtg caccccgatg atctgcaacg tgtggatgat atgctcggcg caaccttaag
    17461 tttgcatggc tggcgcttgc ggggcgatcc cacactccat cctggcggct gtaaagtctc
    17521 cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc tggcaagaac tctgccgtct
    17581 ggcagcacca ggagtggtgt aatgaccacg cgcctgactc gctggctaac cacgctggat
    17641 aactttgaag ccaaaatggc gcagttgcct gcggtacgtc gctacgggcg attaactcgc
    17701 gctaccgggc tggtgctgga agccaccgga ttacaattgc cgctcggcgc aacctgtgtc
    17761 attgagcgcc agaacggcag cgaaacgcac gaagtagaaa gcgaagtcgt tggctttaac
    17821 ggtcaacggc tgtttttaat gccgctggag gaagtcgaag gtgtcctgcc cggcgcgcgt
    17881 gtttatgcca aaaacatttc ggcagaaggg ctgcaaagcg gcaagcagtt gccgctcggt
    17941 ccggcgttat taggtcgtgt tctggacggc agcggtaaac cgctcgatgg cctcccctcc
    18001 cccgatacga cggaaaccgg tgcgctgatt accccaccat ttaacccgtt gcaacgtaca
    18061 ccgattgaac atgtgctgga caccggcgtg cgcccaatca atgccctgct taccgttggg
    18121 cgtgggcagc gtatggggct gtttgccggg tccggcgttg gtaaaagtgt gctgctgggg
    18181 atgatggcac gttacacccg cgccgatgtc attgtcgtgg gtttgattgg tgaacgcgga
    18241 cgcgaagtaa aagattttat tgagaatatc ctcggtgccg aagggcgtgc acgctccgtg
    18301 gtgattgccg ctccggcgga tgtctctccg cttctgcgaa tgcaaggtgc cgcctatgcc
    18361 acgcgaattg ccgaagattt tcgcgatcgt ggtcagcatg tgttgctgat tatggactcc
    18421 ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg cgattggcga accacctgcc
    18481 actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg cactggtcga gcgtgccgga
    18541 aatggaatta gcggcggcgg ctcgattacc gcgttttata ccgtgctcac cgaaggcgat
    18601 gaccagcagg accccattgc cgactctgcg cgggcaatcc tcgacggtca cattgtgctg
    18661 tctcgccgac tggcggaagc cgggcactat ccggctatcg atattgaagc gtcgatcagc
    18721 cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc gagtgcgcac cttcaaacag
    18781 ctgttgtcga gttttcagcg taaccgcgat ctggttagcg tcggcgcgta tgccaaaggc
    18841 agcgatccga tgctcgacaa agccatcgcc ctgtggccgc agctggaggg ctatttgcaa
    18901 caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc aggggctgga gcgtattttc
    18961 ccgacagtgt cataacccag gagataacgg cagatggcag aacatggcgc gctggcgacc
    19021 ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc gcctgctggg tgaaatgcgt
    19081 cgcgggtgtc agcaggcgga agaacagctc aaaatgctga tcgattatca gaatgaatat
    19141 cgcaataacc tcaacagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat
    19201 cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag
    19261 tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag
    19321 gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc
    19381 gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc
    19441 gcttagcgcc cttaattacc gccgacgttg ataacgccac attgcctggc ggcaaagcca
    19501 gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc attagcaggc gagacaacta
    19561 ccgacaaagc ggccccccag ttgctggtgg caacagataa gcccacgaca aaaggcgagc
    19621 cgctggtcag cgagattctt gccgatgcac aacaagcgga tttactgatc cctgtggatg
    19681 aaacaccgcc tgtcatcaac gacgaacaat ccacatcaac accattaacc accgctcaaa
    19741 cgatgacgtt ggctgcggtg gctgacaaca atacggcaaa agacgaaaaa gcggatgatc
    19801 tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc gatgttgccg ggttttgaca
    19861 atacgcccaa agtgactgat gtgccgtcaa ccgtgttacc ggcagagaaa ccaacgctct
    19921 tcacaaaact gacttctgcg caactcacaa cagcacagcc tgatgatgcc cccggcacgc
    19981 cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca
    20041 caccttcacc ggtgaccgct gccgccagcc cgctaatcac tccacaccag acacagccac
    20101 tgcccaccgt cgccgtgcct gtgttgagtg caccgctggg ttctcacgaa tggcaacaat
    20161 cattaagcca gcatatttcg ctgttcaccc gccagaggca acaaagtgca gagttgcgtc
    20221 tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa agtggatgat aaccaggcac
    20281 aaatccagat ggtttcaccg catcagcatg tacgcgccgc gctggaagca gcgcttccgg
    20341 tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac atcagtggcg
    20401 aaagctttag tggtcagcag caggccgctt cccaacaaca gcaaagccaa cgcacagtaa
    20461 accatgaacc tctggcgggg gaagacgacg atacgcttct ggttcccgtc tctatacaag
    20521 ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta
    20581 atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag
    20641 tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg
    20701 atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta
    20761 cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtacc
    20821 ctccccggtc ttctacgcgc tggatacctt cacggtcaat ctgggcgatg cggatcgcgt
    20881 gctttatatc ggcataaccc tgcgcctgaa agatgaagct acccgctcgc ggctgagtga
    20941 gtatttgccg gaagtccgta gccgtttgct gttactgttt tcgcgtcagg atgctgccgt
    21001 actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca cgctttccac
    21061 cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat
    21121 tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga
    21181 atggtgacag cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa agtgacattc
    21241 gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa
    21301 tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgtcgta
    21361 gcccggatat aaccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc
    21421 tgccggtgcc gaccaacctg aaccttatcc atctgaaacc gctgcgcggc acagggctgg
    21481 tggtgttttc accgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac
    21541 gcttcccgac caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc gtcatcaacc
    21601 gcatgttaaa gctggcgctt gaaggctata gcgacgcctg gaaggcgatt aatccgctgg
    21661 aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc acctcgccga
    21721 acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta
    21781 atatctgcct gcctttcagc atgatcgagc cgctacggga attgttggtt aacccgccgc
    21841 tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc
    21901 attcacagct ggagctggtc gccaactttg ccgatatctc gctacgcctg tcgcagattt
    21961 taaaactgaa acccggtgac gtcctgccga tagaaaaacc cgaccgcatc atcgcccatg
    22021 ttgacggcgt cccggtgctg accagtcagt acggcactct caacggtcag tatgcgttac
    22081 ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag
    22141 tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc
    22201 tttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt tccagcaatt
    22261 tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt
    22321 caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac
    22381 gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa ccactggata ttctgatcaa
    22441 cggttattta attgcccagg gcgaagtggt ggtcgttgcc gataaatatg gcgtgcggat
    22501 caccgatatc attactccgt ctgagcgaat gcgccgcctg agccgttagt gatgaataac
    22561 cacgctactg tgcaatcttc cgcgccggtt tctgctgcgc cactgctgca ggtgagcggc
    22621 gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggttggga
    22681 tttgtcccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc
    22741 gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc
    22801 gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg
    22861 cagcccgatt ttcagtcggt tatgaaaaac ttgcttaagc gtagcgggag atcctgatgc
    22921 gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt tacgcccctc gccttcgcgc
    22981 aactgccggg tatcaccagc cagccgctgc ctggcggtgg acaaagctgg tcgctcccgg
    23041 tgcaaacgct ggtgttcatc acctcgttga cgtttattcc ggcaatttta ctgatgatga
    23101 ccagtttcac ccgcatcatc attgtttttg gtttattgcg taacgcgttg ggaacaccct
    23161 ccgcgccacc taaccaggta ttgctgggac tggcgctgtt tttgaccttt tttattatgt
    23221 cgccggtgat cgataaaatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct
    23281 caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga
    23341 cccgcgaggc ggatttaggg ttgtttgcca gactggcgaa tactggcccg ttgcagggac
    23401 ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag ttgaaaaccg
    23461 catttcagat aggcttcacg attttcattc cttttttgat tatcgacctg gtgattgcca
    23521 gcgtgttgat ggcattgggg atgatgatgg ttcccccagc cacaattgct ctgcccttta
    23581 aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg ctggcgcaga
    23641 gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg gggactgaag
    23701 cgatgaaagt cgcgctggca ctggctgccc cactgttgct ggtggcgctg gtcacgggtc
    23761 ttatcatcag tattttgcag gccgccaccc agattaacga aatgacgctg tcgtttattc
    23821 cgaaaatcat cgccgtattt atcgccatta ttattgccgg accgtggatg ctcaatctgt
    23881 tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg taggccgtac
    23941 aatgatgcag gtaacaagcg atcaatggct ttcctggtta agcctgtact tctggccgtt
    24001 actgcgcgtg ctggcgctca tttctaccgc accaattctg agcgaacgca gcgtgccgaa
    24061 acgggtaaaa ctgggtctgg caatgatgat cacgttcgct attgccccat cattacctgc
    24121 caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtgcagc agatcctgat
    24181 cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggtga
    24241 aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt gtcgatccgg ccagccatct
    24301 caatatgccc gttttagcgc gtatcatgga tatgctggcg ttactgctgt tcctgacatt
    24361 taacggtcat ttatggttga tttcactgct ggccgatacc tttcacaccc tgccaattgg
    24421 tggcgaaccg ttgaacagca atgcgtttct ggcactcacc aaagcaggga gtttgatttt
    24481 ccttaacggg ctgatgctgg cattaccgct cattactctg ctgctgaccc tgaatctggc
    24541 attaggctta cttaatcgta tggccccgca attatccatt tttgttattg gatttccatt
    24601 aattctgact gtcggtatct ctttaatggc ggcattaatg ccgttaattg cgcctttttg
    24661 cgaacattta ttcagtgaga tgtttaattt gctggctgat attattagtg aattgccatt
    24721 aatataattc cgtaacgttt atcatgttat cctaaggatt atccgaaaaa taatacctac
    24781 gaacatcttc caggatactc ctgcggcgaa atatttgttt taagctcact cacatatcgc
    24841 aacatttact ttactttaag acaattccag gcaaattata caacacttta cgggatagta
    24901 agtccgcctg aaaaatcgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc
    24961 atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt
    25021 caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga
    25081 gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag
    25141 cttgtgattc acagcgccct tcagtggtgt ttattaatga ggactgtttc atccacgatg
    25201 cttctaacag tcagcgtatc aagctcatca ttaatcaaca tcccaatacg ttatttatcg
    25261 tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat
    25321 tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga
    25381 aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg
    25441 aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc tctgaccaaa
    25501 tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa
    25561 cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta
    25621 tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc aggccgaatt
    25681 agattactct gctttctcca caaagatacc gtcctgatgc cctgcttcat taaagaacca
    25741 gatgccgagc gggtagtctt ccagcgaaac caggtacatt gtgccttcac taaactcctc
    25801 aactgccagt accacgccag gacgacgcgg accgccatcc gttttgactg ttacccgatc
    25861 attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc
    25921 tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc
    25981 aggcggactt aacgactggc cgcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa
    26041 accgcaaaag agtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg
    26101 ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa
    26161 cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa
    26221 ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc
    26281 ccgaccctga gggggtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
    26341 acaccaggaa atctgatgtg ttcatcacct tatccgcaat ttttttcgct gacaagaaaa
    26401 tattcgtgat atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct
    26461 tggtggctct ggcgtcaacg ggatcgctcc cgaaaaaatc atctttgata atttgtgctg
    26521 ttttgagaat catcctggta aggggagtac tgcgcaacca tgctttcaat tcaacaacca
    26581 ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa
    26641 ccagctgccc cctggctcag ccgtttacgc gaagcgaatg ttcccgtcat tctctgtagc
    26701 agtaagacat cagccgaaat gctgtacttg caaaaaacgt tggggctaca aggtttaccg
    26761 ctgattgccg aaaatggcgc agtgatccag cttgctgagc aatggcagga tatagacggt
    26821 tttccacgca tcatctcagg tattagccat ggcgaaatca gccaggtttt aaatacgcta
    26881 cgcgagaaag aacattttaa attcacaact tttgatgatg tcgacgatgc aaccatcgcc
    26941 gaatggacgg gattaagccg tagccaggcg gcgctgacgc agctacatga ggcgtcggta
    27001 acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttaccgctcg tctgaacgaa
    27061 ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tcctggatgc ctctgccgga
    27121 aaagatcagg ctgccaactg gattatcgcg acctatcaac aatcgtcagg caaacgccca
    27181 accacacttg gcctgggcga tgggccaaac gatgcgccct tactggaggt aatggattac
    27241 gcggtgattg tgaaagggtt aaaccgtgaa ggggtgcatc tgcatgatga ggatccggcc
    27301 cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt
    27361 ttctccgccc attaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgtttagcg
    27421 agataaagtc gacggtcggc cagtgactgg agttgttcaa aatcataatc accggtttcc
    27481 tcgctgctgc ttacccccag cgaggcactg atgcgtatcg tcgtactctt ggcgatcaac
    27541 atctcttttt catttagctt caggcgaata cgttctgcaa cctccgcagc ctgcgtcagg
    27601 ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacatcctgc
    27661 gctcgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga
    27721 tgaccaaagc ggtcattaat cgctttaaaa tggtcaaggt cgacctgaat gacagaaaaa
    27781 ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca
    27841 cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc agtgcaacga gctttgcaaa
    27901 acatacatgt tgctgaccat ccggcgaatc acataccagg agatgagtaa catggtggta
    27961 aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct
    28021 tcgcttagcg tatggacgcg caccagcaca ccgtcgaaat ggtccagacg ttcccagcta
    28081 acatagcgac tgttcatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc
    28141 aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc
    28201 aataatctca gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg
    28261 tttcttaaaa attgctgcat ggtacgcacg ggaatactca tccccagcac gccataccag
    28321 tagttttcac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt
    28381 tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg
    28441 acaaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg cgtaaacaag
    28501 gtcggctgcg tcgaaacgta aaatccggca cgcgagacat acatcgcctg ttcaaccatc
    28561 gacgaggagt tgtgtgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca
    28621 ttgtcgaggc tttcattttc gcgagacagg agattcccct cgctgactaa tgcatccgac
    28681 acaccattga ctggcagggt gcgtcgtcgg ttgagttcga tttgccaggc gtgctcgtcg
    28741 cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgctacg
    28801 agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc
    28861 ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct
    28921 tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag
    28981 cagagattaa cgacatgacc aggccctagg cggcgtgcga gttttttcaa ccagctctgg
    29041 ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct
    29101 ggcataacct ggcgatagcg taccgcaaaa cagcgataat tttataatgg tggcatgata
    29161 agacgcgaca gacatcgata gttttataac gtaggcatga taagacgcga tagcgtcgca
    29221 tcaggcaatt cagtgcagac actggcacac ccgaccggat gcgacgtgag cgccacatcc
    29281 ggtcgggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga
    29341 acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata ccaacgacac
    29401 tcaaccatac cgctggagta tccggtgaca atcatccgcg ggccgccctc tttaaccgta
    29461 acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac
    29521 cttagttgaa gatggcgaat tttgcataat agcctgtgcg agtattaatg tgcctgaccg
    29581 cgtattttcg ctacggcttt cactcccagc accacgatgc cgccgatgat aaatccaaga
    29641 atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgaaa
    29701 tgttcaatgg cgtgatgcag cggcgcaatt ccatgtacca caatcccacc gccgacgagg
    29761 aacatcgcca gcgtgccgac aatcgataac gccttcatca accagggcgc aatgatcaat
    29821 aaccctttgc ctaatgcctg catcagcgcg ctggattttt ccgccagcca ataccccagg
    29881 tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg
    29941 atgcctgaaa gcaccagcac ctgattaagc aacggcgctt cggccacaat ccccagcgtg
    30001 atggcgacga tttccgcaga caatataaaa tcggtacgaa tcgccccttt tattttgtct
    30061 ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg actctgcgcc
    30121 ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac cccttcaaag
    30181 cagagaaacg cgccgccaat catcaacagt ggcgtaatcg cccacgggat aaacgcactg
    30241 atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt cgccacgccc
    30301 cagaccacgg gaagttcccg gttggcgcgc acgcctgcaa cttgttgcgc attgagcgat
    30361 aagtcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacggagata
    30421 tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc
    30481 actccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat
    30541 acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct ctcgccagca
    30601 gaatgatttt cacgtttact atgaaccacc tttttatttc agccgtgagg gattatgcgt
    30661 ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca
    30721 acctattttg tcattcggat tggcgtggaa agctggcctc cgttaatgat ggcgggcgtt
    30781 cgattcctgg cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactt
    30841 cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct ggctgtcggt
    30901 aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgttgtg
    30961 gttgcaaccg tgcccctctt taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc
    31021 aaactggaat gggtgggtat cgccattggg cttgccggaa tcatcatgct caatagcggt
    31081 ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc gattagctgg
    31141 gcgtttggct cggtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg
    31201 attgagatgc tggcggctgg cgtggtgtta atgattgcgt cgatgattgc gggtgaaaaa
    31261 ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg gctatctggc gctgtttggt
    31321 tcgattatcg ccattaacgc ttatatgtat ttaatccgca acgtcagtcc ggctctcgcc
    31381 accagctacg cctacgttaa cccagtggtc gcggtcttgc tgggtacggg gctgggtggc
    31441 gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc ggtggtactg
    31501 gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt tattccggat
    31561 gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca
    31621 gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc gcccgcgtaa
    31681 cgcacattcc cagacaatca atacgcgcca accgagttcc tgcaaatggc tgatatcacg
    31741 acgatcgcgc tcgacatttt tacctatctt ctccagccaa aaatctgttc gcgtggcggg
    31801 gactttaaac aggtagcaat ggtgatggtg ccagaagcaa ccgtgggcaa atatcacaca
    31861 gcgaaattca tcaaggacaa aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa
    31921 cgccaggccc tgcccagtta acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt
    31981 ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg
    32041 cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg
    32101 cggcaaagac cggcacgacc accgagttac cgaactggcg ataagcctga gtgtccgaaa
    32161 ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc gcacattccc
    32221 gaggcgttaa ccgacgtggg cgatgttgct gattcagcgg atcgtcaaag tctttctcac
    32281 ccgttgccat atcccagccg cgatcgatta aaatttccgc gccatctttg taataacgcg
    32341 cagacagcgt acgcgtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc
    32401 cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac agtaccggcg
    32461 tcaggatata tttcgcctcg accatcgggt ccagcagctg cgccagcgtc actcgctgtg
    32521 cagggaaaca tttgctgata tcacgcagag taaaatcggc tttcagattc agatcgcgac
    32581 gaaaacccac cagcacgatg cgttcacggt gctgcggcag aaaatgtttg ccgtcgatga
    32641 ttttcggatc gtccggcccg ttatcttctg catcagccac gtcatagccc agttcgtcca
    32701 gcgtctgcat gatgatgcgg aacgttttac cctggtcgtg acttttcagg tttttgacgt
    32761 tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca
    32821 gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata
    32881 cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg tgttcaggaa
    32941 tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga tggctgaggg
    33001 tgatgtcgcg gatatcctca ttaaaatgat gcgtcgccgg gtcgcaataa tggttggctt
    33061 tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt ccaccaatcg
    33121 attcaaaacc gcgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat
    33181 aatgcggatg atgtgccggt ggtttgggta acaacgtttg cagatgggcg aactctttct
    33241 cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc
    33301 agtgattctc acccacacca ttaagctgcg ccaccagcgt tttaacatca taaatttgca
    33361 gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg gttactgata
    33421 tattttcctg catagattta accggccatc tgagatggcc gacaggttaa cataattact
    33481 gccgtttagg gccacgcgct cagccagcag gtgaaaacgc atctatcacc ttgtgatcga
    33541 taccttcatt ctcccccgcc agttcggcac cgagcttcgc cataaactcg actaaaaatt
    33601 gcgcgttgtg ctgtgccagc tgcttgcctc ttgcggtttg catggtttgc ggtaatttca
    33661 gtaactttgt ctggaaatga tccagcgcgt agcgtttatc atcaagcggg cgatgctgtg
    33721 cgaacggatc ttcgccatca aacagcgcca agcccaatgc ccctgaaacg gcaaatacgc
    33781 gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt
    33841 ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg
    33901 cctcaatttt ctccgccgga aattgcacga actctt
//
LOCUS       DAZFDP010000033        33156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFDP010000033 DAZFDP010000000
VERSION     DAZFDP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHJ1G_001748"
     CDS             complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHJ1G_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4763620.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2151..3017
                     /gene="yghU"
                     /locus_tag="ACHJ1G_001749"
     CDS             2151..3017
                     /gene="yghU"
                     /locus_tag="ACHJ1G_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4763621.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="ACHJ1G_001750"
     CDS             complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="ACHJ1G_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4763622.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="ACHJ1G_001751"
     CDS             complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="ACHJ1G_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4763623.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="ACHJ1G_001752"
     CDS             complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="ACHJ1G_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4763624.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4217..4711)
                     /gene="hybD"
                     /locus_tag="ACHJ1G_001753"
     CDS             complement(4217..4711)
                     /gene="hybD"
                     /locus_tag="ACHJ1G_001753"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4763625.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="ACHJ1G_001754"
     CDS             complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="ACHJ1G_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4763626.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="ACHJ1G_001755"
     CDS             complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="ACHJ1G_001755"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4763627.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="ACHJ1G_001756"
     CDS             complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="ACHJ1G_001756"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4763628.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="ACHJ1G_001757"
     CDS             complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="ACHJ1G_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4763629.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9875..10162)
                     /gene="yghW"
                     /locus_tag="ACHJ1G_001758"
     CDS             complement(9875..10162)
                     /gene="yghW"
                     /locus_tag="ACHJ1G_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4763630.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10281..11168)
                     /gene="yghX"
                     /locus_tag="ACHJ1G_001759"
     CDS             complement(10281..11168)
                     /gene="yghX"
                     /locus_tag="ACHJ1G_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4763631.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11325..12365
                     /gene="gpr"
                     /locus_tag="ACHJ1G_001760"
     CDS             11325..12365
                     /gene="gpr"
                     /locus_tag="ACHJ1G_001760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4763632.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12405..12899)
                     /gene="yqhA"
                     /locus_tag="ACHJ1G_001761"
     CDS             complement(12405..12899)
                     /gene="yqhA"
                     /locus_tag="ACHJ1G_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4763633.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13090..13974
                     /gene="yghA"
                     /locus_tag="ACHJ1G_001762"
     CDS             13090..13974
                     /gene="yghA"
                     /locus_tag="ACHJ1G_001762"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4763634.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14246..14671)
                     /gene="exbD"
                     /locus_tag="ACHJ1G_001763"
     CDS             complement(14246..14671)
                     /gene="exbD"
                     /locus_tag="ACHJ1G_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4763635.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14678..15412)
                     /gene="exbB"
                     /locus_tag="ACHJ1G_001764"
     CDS             complement(14678..15412)
                     /gene="exbB"
                     /locus_tag="ACHJ1G_001764"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4763636.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15664..16851
                     /gene="metC"
                     /locus_tag="ACHJ1G_001765"
     CDS             15664..16851
                     /gene="metC"
                     /locus_tag="ACHJ1G_001765"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4763637.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16991..17650
                     /gene="yghB"
                     /locus_tag="ACHJ1G_001766"
     CDS             16991..17650
                     /gene="yghB"
                     /locus_tag="ACHJ1G_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4763638.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17690..18589)
                     /gene="yqhC"
                     /locus_tag="ACHJ1G_001767"
     CDS             complement(17690..18589)
                     /gene="yqhC"
                     /locus_tag="ACHJ1G_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4763639.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18783..19946
                     /gene="yqhD"
                     /locus_tag="ACHJ1G_001768"
     CDS             18783..19946
                     /gene="yqhD"
                     /locus_tag="ACHJ1G_001768"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4763640.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20051..20878
                     /gene="dkgA"
                     /locus_tag="ACHJ1G_001769"
     CDS             20051..20878
                     /gene="dkgA"
                     /locus_tag="ACHJ1G_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4763641.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21078..22004
                     /gene="yqhG"
                     /locus_tag="ACHJ1G_001770"
     CDS             21078..22004
                     /gene="yqhG"
                     /locus_tag="ACHJ1G_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO4763642.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22055..22312
                     /gene="yqhH"
                     /locus_tag="ACHJ1G_001771"
     CDS             22055..22312
                     /gene="yqhH"
                     /locus_tag="ACHJ1G_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4763643.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22355..24574)
                     /gene="ygiQ"
                     /locus_tag="ACHJ1G_001772"
     CDS             complement(22355..24574)
                     /gene="ygiQ"
                     /locus_tag="ACHJ1G_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4763644.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24685..26097)
                     /gene="ftsP"
                     /locus_tag="ACHJ1G_001773"
     CDS             complement(24685..26097)
                     /gene="ftsP"
                     /locus_tag="ACHJ1G_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4763645.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26172..26909)
                     /gene="plsC"
                     /locus_tag="ACHJ1G_001774"
                     /gene_synonym="parF"
     CDS             complement(26172..26909)
                     /gene="plsC"
                     /locus_tag="ACHJ1G_001774"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4763646.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27142..29400)
                     /gene="parC"
                     /locus_tag="ACHJ1G_001775"
     CDS             complement(27142..29400)
                     /gene="parC"
                     /locus_tag="ACHJ1G_001775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4763647.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29946..30428)
                     /gene="ygiV"
                     /locus_tag="ACHJ1G_001776"
     CDS             complement(29946..30428)
                     /gene="ygiV"
                     /locus_tag="ACHJ1G_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4763648.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30481..30873)
                     /gene="ygiW"
                     /locus_tag="ACHJ1G_001777"
     CDS             complement(30481..30873)
                     /gene="ygiW"
                     /locus_tag="ACHJ1G_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4763649.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31025..31684
                     /gene="qseB"
                     /locus_tag="ACHJ1G_001778"
     CDS             31025..31684
                     /gene="qseB"
                     /locus_tag="ACHJ1G_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4763650.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31681..33030
                     /gene="qseC"
                     /locus_tag="ACHJ1G_001779"
     CDS             31681..33030
                     /gene="qseC"
                     /locus_tag="ACHJ1G_001779"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4763651.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33076..>33156)
                     /locus_tag="ACHJ1G_001780"
     CDS             complement(33076..>33156)
                     /locus_tag="ACHJ1G_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFO4763652.1"
                     /translation="WVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         8127 a         8874 c         8383 g         7772 t
ORIGIN      
        1 tattggaata tataaatatt gaatattttg attaatcccc gtactaatta ttcttcataa
       61 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
      121 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      181 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      241 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      301 tgatggctga ccaggttgat attgctacca cagcgaccgg caatcggttt cactgcgtaa
      361 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      421 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      481 ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      541 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      601 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg tccttcccca
      661 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      721 tcaaagccct cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      781 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      841 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      901 caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc gttgtattcg
      961 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
     1021 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1081 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1141 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1201 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1261 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1321 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1381 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1441 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1501 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1561 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1621 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1681 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1741 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1801 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1861 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1921 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1981 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     2041 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2101 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2161 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2221 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2281 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2341 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2401 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2461 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2521 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2581 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2641 tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2701 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt gcgggcgatg
     2761 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg ttaggcggcg
     2821 tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2881 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2941 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     3001 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3061 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
     3121 gtatctacga aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg
     3181 tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc
     3241 agcacccact ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg
     3301 tcgcgcttga taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc
     3361 agcacctggc ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt
     3421 ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac
     3481 tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg
     3541 ggtttataga cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca
     3601 aaactaaaac ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg
     3661 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
     3721 tggcaaagag acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg
     3781 ccctgcgtgt ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc
     3841 atccgtcagg cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga
     3901 acaggagaga tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata
     3961 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
     4021 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
     4081 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
     4141 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
     4201 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt
     4261 cacgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
     4321 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
     4381 gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag
     4441 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
     4501 gcgtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
     4561 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
     4621 tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa
     4681 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
     4741 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
     4801 acgcaccact tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc
     4861 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
     4921 tgcctggtag ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg
     4981 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac
     5041 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag
     5101 gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg
     5161 cagctgtgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
     5221 cagtttgttc tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg
     5281 acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
     5341 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc
     5401 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
     5461 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa
     5521 caggaagctg ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
     5581 acctttaccg cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt
     5641 ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg
     5701 ctcaaggttc aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc
     5761 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
     5821 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
     5881 tttcatcgct gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac
     5941 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
     6001 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
     6061 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
     6121 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
     6181 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
     6241 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
     6301 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
     6361 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
     6421 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
     6481 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
     6541 tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc
     6601 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa
     6661 tcattacgca gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca
     6721 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
     6781 ataagctcgc caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc
     6841 agcttgacga acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc
     6901 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
     6961 ggcaacattt catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt
     7021 gaccccattg aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac
     7081 atcaccttgt tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga
     7141 gcaaactcca gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt
     7201 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
     7261 acgtcgatag tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc
     7321 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
     7381 cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac
     7441 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
     7501 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
     7561 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
     7621 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
     7681 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
     7741 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
     7801 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
     7861 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
     7921 tttcgcctcc gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac
     7981 ttctacgcag ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa
     8041 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg
     8101 gcaggcgacc atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc
     8161 ggttttcgga tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac
     8221 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
     8281 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
     8341 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
     8401 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
     8461 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
     8521 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
     8581 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
     8641 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg
     8701 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
     8761 agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga
     8821 ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg
     8881 taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca
     8941 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
     9001 tgaatcagac ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg
     9061 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
     9121 ggaatattga taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg
     9181 ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct
     9241 gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa
     9301 taaataccgt tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg
     9361 tacttctcaa gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
     9421 acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
     9481 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
     9541 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
     9601 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
     9661 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata
     9721 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
     9781 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
     9841 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca
     9901 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
     9961 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
    10021 cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
    10081 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
    10141 ctttaccaaa atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga
    10201 tattttaggc aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa
    10261 tccagtaaga gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc
    10321 caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga
    10381 ttaacccccg gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg
    10441 taagcaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
    10501 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
    10561 gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg
    10621 gtaataccca ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag
    10681 aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca
    10741 tttcccggat aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc
    10801 ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc
    10861 actaccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc
    10921 tcgccgtgac catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta
    10981 aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc
    11041 gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc
    11101 ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa
    11161 cgcggcatcg ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt
    11221 tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa
    11281 actagaaaca ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga
    11341 atcccgaacg ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc
    11401 ccgcgttatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc
    11461 gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt
    11521 acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg
    11581 ccgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc
    11641 cttacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc
    11701 gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga
    11761 tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg
    11821 ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga
    11881 aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa
    11941 gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg
    12001 ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc
    12061 gtgaagggaa taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca
    12121 gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt
    12181 taagctggtt gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg
    12241 agcaactaga ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg
    12301 cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata
    12361 aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga
    12421 gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg
    12481 acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg
    12541 tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc
    12601 attttcccca gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa
    12661 ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc
    12721 agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg
    12781 ttaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag
    12841 tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata
    12901 cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca
    12961 acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc
    13021 ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg
    13081 agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc
    13141 aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc
    13201 gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc
    13261 gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg
    13321 atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa
    13381 tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg
    13441 ctggttcacg aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg
    13501 aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt
    13561 gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa
    13621 ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg
    13681 gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc
    13741 gcggagaaag gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa
    13801 atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag
    13861 cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg
    13921 agctacgtca ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat
    13981 gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa
    14041 aacgccttat ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc
    14101 ctgataagcg tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt
    14161 atccggccta caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga
    14221 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
    14281 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
    14341 cgacggtttt atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
    14401 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
    14461 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
    14521 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
    14581 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
    14641 cgtcgaggtt ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg
    14701 cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca
    14761 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
    14821 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
    14881 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
    14941 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
    15001 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
    15061 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
    15121 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
    15181 aatcagcggc gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct
    15241 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
    15301 aagtgactac ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
    15361 gctgatacat accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc
    15421 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag
    15481 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
    15541 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
    15601 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc
    15661 gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
    15721 actctcggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg
    15781 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
    15841 ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
    15901 tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa
    15961 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
    16021 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
    16081 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc
    16141 accatggaag tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat
    16201 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
    16261 ggcatcgatg tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg
    16321 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg
    16381 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
    16441 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa
    16501 catccgcaag ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc
    16561 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
    16621 aataatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg
    16681 tggggcgggt atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc
    16741 ccccaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
    16801 gtcgacgatc tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac
    16861 ttttggacaa ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc
    16921 aaggcgtccc ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac
    16981 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
    17041 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
    17101 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
    17161 aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc
    17221 cgcagcaagt ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa
    17281 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
    17341 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
    17401 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa
    17461 ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat
    17521 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
    17581 tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg
    17641 taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct
    17701 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
    17761 cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca
    17821 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
    17881 acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg
    17941 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca
    18001 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
    18061 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
    18121 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
    18181 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
    18241 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg
    18301 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
    18361 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
    18421 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
    18481 cgccatacaa caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca
    18541 gtttattaac tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt
    18601 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
    18661 ggaggaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
    18721 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
    18781 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
    18841 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca
    18901 gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
    18961 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
    19021 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
    19081 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
    19141 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
    19201 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
    19261 agcaggcgtt ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata
    19321 cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg
    19381 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
    19441 ttttgctgac gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
    19501 tgcgcgccaa cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg
    19561 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
    19621 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
    19681 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg
    19741 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
    19801 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
    19861 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc
    19921 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
    19981 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
    20041 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
    20101 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
    20161 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
    20221 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
    20281 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
    20341 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
    20401 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
    20461 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
    20521 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc
    20581 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
    20641 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt
    20701 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
    20761 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat
    20821 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca
    20881 tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
    20941 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
    21001 atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
    21061 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
    21121 ccgtacacgc acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga
    21181 actataaatc agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt
    21241 ctgcgcgtat tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg
    21301 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa
    21361 gcgttgcgat aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct
    21421 ttaaagatga taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt
    21481 gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    21541 atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg
    21601 ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
    21661 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
    21721 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    21781 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    21841 gtattcatgt tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac
    21901 agcggcgcga aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    21961 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
    22021 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    22081 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct
    22141 ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
    22201 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    22261 tgctcaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    22321 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    22381 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    22441 caggcgtctg acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc
    22501 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    22561 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
    22621 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    22681 tcggtacgaa gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt
    22741 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
    22801 ggaaacgatg ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg
    22861 ggtgcgcgga gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag
    22921 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata
    22981 acggcccttc ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg
    23041 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    23101 tgaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    23161 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    23221 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    23281 gatcggaaat tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa
    23341 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga
    23401 aagagcagcc gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag
    23461 ccggaatacg ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa
    23521 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    23581 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
    23641 gcgaagcatg agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata
    23701 cgtaggtttt ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct
    23761 gctttttcgg cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg
    23821 ggattgggtc gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag
    23881 gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct
    23941 cgcccatcgc cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca
    24001 gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat
    24061 aatgcgcggt acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc
    24121 acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga
    24181 cgttatccgg tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga
    24241 tcatcgaatc catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca
    24301 taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg
    24361 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    24421 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    24481 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    24541 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    24601 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    24661 gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga
    24721 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    24781 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    24841 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc
    24901 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    24961 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    25021 acccagacta atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa
    25081 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    25141 cagggtagaa accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct
    25201 cgccgcttcg ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc
    25261 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    25321 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    25381 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    25441 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    25501 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    25561 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    25621 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    25681 cagagtagct gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat
    25741 catccgtgcc ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga
    25801 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    25861 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    25921 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    25981 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    26041 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    26101 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    26161 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    26221 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    26281 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    26341 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    26401 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    26461 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    26521 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    26581 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    26641 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    26701 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    26761 ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    26821 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    26881 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    26941 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    27001 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    27061 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg
    27121 aaacaggccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    27181 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    27241 ttcgccagtg actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg
    27301 aatggtcagc gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg
    27361 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    27421 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    27481 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    27541 agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc
    27601 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    27661 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    27721 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    27781 gttgctctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    27841 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    27901 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    27961 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    28021 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    28081 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    28141 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    28201 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    28261 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    28321 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    28381 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    28441 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    28501 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    28561 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    28621 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    28681 acgcactgaa ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat
    28741 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt
    28801 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    28861 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    28921 acgagcaggc agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    28981 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    29041 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    29101 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    29161 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    29221 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    29281 aggtttaaga ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    29341 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    29401 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    29461 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    29521 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    29581 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    29641 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    29701 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    29761 tgccataagt gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat
    29821 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    29881 ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    29941 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    30001 gcaagattgg tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc
    30061 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    30121 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    30181 ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    30241 gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    30301 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    30361 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    30421 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    30481 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    30541 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    30601 gtggtcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    30661 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    30721 gctttctacg gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt
    30781 tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc
    30841 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    30901 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg
    30961 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    31021 aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg
    31081 ccttagtaaa atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    31141 gctttatagc gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    31201 tcgcgatatt ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac
    31261 cgcgcgcgat gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    31321 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac
    31381 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    31441 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    31501 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    31561 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    31621 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    31681 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    31741 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    31801 gatgaattgt tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc
    31861 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    31921 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac
    31981 gatggcgata acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg
    32041 caactggtgg gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa
    32101 tatcgcatcg ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    32161 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    32221 ctattgggtc gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac
    32281 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt
    32341 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt
    32401 acctccgacg cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa
    32461 gttgcgcagc tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat
    32521 tacgggatcg atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac
    32581 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg
    32641 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    32701 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    32761 ctggataacg ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct
    32821 gataatttca tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt
    32881 ggcgaacgct tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg
    32941 attgtccagc gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa
    33001 ggtggatttg aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga
    33061 ctctcataga ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca
    33121 aaccagacaa ataccgagcg agaatacata taccca
//
LOCUS       DAZFDP010000034        32467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFDP010000034 DAZFDP010000000
VERSION     DAZFDP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(282..2303)
                     /gene="fyuA"
                     /locus_tag="ACHJ1G_001781"
     CDS             complement(282..2303)
                     /gene="fyuA"
                     /locus_tag="ACHJ1G_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO4763653.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(2434..3999)
                     /gene="ybtE"
                     /locus_tag="ACHJ1G_001782"
     CDS             complement(2434..3999)
                     /gene="ybtE"
                     /locus_tag="ACHJ1G_001782"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO4763654.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(4003..4806)
                     /gene="ybtT"
                     /locus_tag="ACHJ1G_001783"
     CDS             complement(4003..4806)
                     /gene="ybtT"
                     /locus_tag="ACHJ1G_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO4763655.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(4803..5903)
                     /gene="ybtU"
                     /locus_tag="ACHJ1G_001784"
     CDS             complement(4803..5903)
                     /gene="ybtU"
                     /locus_tag="ACHJ1G_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO4763656.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(5900..15382)
                     /gene="irp1"
                     /locus_tag="ACHJ1G_001785"
     CDS             complement(5900..15382)
                     /gene="irp1"
                     /locus_tag="ACHJ1G_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO4763657.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGSVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWHTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELASYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     SLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLASHVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALSCGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAVS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRKTSAWHDAWDYWQEKLPQLPLAPELPMVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFTAHP
                     EAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGFIEG
                     LSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPGVNR
                     PDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSALEK
                     QVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHSTLSD
                     FCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQAKS
                     PQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRMVLI
                     DPVCRQDFCCENRAALLRLLAEGETPLALPEHFDQQTPDSQLADFISLAKTAGMVSQN
                     LTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETELQGWINN
                     ADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            complement(15470..21577)
                     /gene="irp2"
                     /locus_tag="ACHJ1G_001786"
     CDS             complement(15470..21577)
                     /gene="irp2"
                     /locus_tag="ACHJ1G_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO4763658.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(21768..22727)
                     /gene="ybtA"
                     /locus_tag="ACHJ1G_001787"
     CDS             complement(21768..22727)
                     /gene="ybtA"
                     /locus_tag="ACHJ1G_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO4763659.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGIAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            22984..24696
                     /gene="ybtP"
                     /locus_tag="ACHJ1G_001788"
     CDS             22984..24696
                     /gene="ybtP"
                     /locus_tag="ACHJ1G_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO4763660.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            24683..26485
                     /gene="ybtQ"
                     /locus_tag="ACHJ1G_001789"
     CDS             24683..26485
                     /gene="ybtQ"
                     /locus_tag="ACHJ1G_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO4763661.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAACAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            26478..27758
                     /gene="ybtX"
                     /locus_tag="ACHJ1G_001790"
     CDS             26478..27758
                     /gene="ybtX"
                     /locus_tag="ACHJ1G_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO4763662.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            27786..29090
                     /gene="ybtS"
                     /locus_tag="ACHJ1G_001791"
     CDS             27786..29090
                     /gene="ybtS"
                     /locus_tag="ACHJ1G_001791"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO4763663.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(29284..30199)
                     /locus_tag="ACHJ1G_001792"
                     /pseudo
     CDS             complement(29284..30199)
                     /locus_tag="ACHJ1G_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(30361..30436)
                     /locus_tag="ACHJ1G_001793"
     tRNA            complement(30361..30436)
                     /locus_tag="ACHJ1G_001793"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30401..30403),aa:Asn,seq:gtt)
     gene            complement(30537..31334)
                     /gene="mtfA"
                     /locus_tag="ACHJ1G_001794"
     CDS             complement(30537..31334)
                     /gene="mtfA"
                     /locus_tag="ACHJ1G_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4763664.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            31428..31517
                     /locus_tag="ACHJ1G_001795"
     tRNA            31428..31517
                     /locus_tag="ACHJ1G_001795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31462..31464,aa:Ser,seq:cga)
     gene            complement(32185..>32467)
                     /locus_tag="ACHJ1G_001796"
     CDS             complement(32185..>32467)
                     /locus_tag="ACHJ1G_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO4763665.1"
                     /translation="TSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPKYIQFSDH
                     IIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         6795 a         8898 c         9678 g         7096 t
ORIGIN      
        1 ttgtttgtct gtgcttttat attctggaga tgtgccttgt atataggatt gtttgttggt
       61 tgtatttttt aatgttgcac tgttgtgaat ttttattttg atatgattat ttctttagta
      121 taaggagaga aaggaacgag aaagttaaat ctatttatcc taatcgcaat ttcatttttt
      181 aaataatcac tatggattaa tattttttgc gactctgctg agttactggt tttaactagt
      241 ggtgtaaaag ggataccttt tcggtatccc ttttacaata atcagaagaa atcaattcgc
      301 gtattgatac cgacggtgcg acccatattg acctgcgcga cggcgctgct gccgttcatg
      361 tagccatagg tacggtaacg acggtcgaac aggttatcga catagacgga aatgttcatc
      421 cgttcagtcg cctgccagcc caggctgctg tccagggtgg cataggtgcc ttgccgcaac
      481 tggttgtcgc catcgaaata atgcggcccg accagattaa ccgccagtcg gggcatcagt
      541 gcgccatagc gcgtatcaat cacgccgttc acgctgcttc ccgcgccata acgtggtacg
      601 aacggcaccc ggttaccgtg atacaactca ctgtcattgg tgaattcgga acggatcacg
      661 ttgccattga tatcccatga ccagcctggc gcaaaccgcc acttcgcttc aagctcaacg
      721 ccggtggcgt cggctttacc cgcattgctt aatgtctgca tcccgtccgg gccagagtaa
      781 agctgcatgt ctttggtgtg ggtataaaac gtcgcggctt gcagcgtgac gtcagcggtt
      841 tcgtagcggg tgccaagttc atagttgatg gatttctcgg cgacgaacgg tttggcatca
      901 agacccgcag taggcacgat gttgtacccg gaaggtttat atccctgggc tacacgggta
      961 tacactctcc agtcatcggt cagcatatag cctgcggata gctgcccgag cacctgatcg
     1021 tcattgctct taccctggtc gccaaacggg ttgccgagca tgctgccgtg atattgtgta
     1081 ctggatttat catgcgagaa gcgcacgccg ccgccgatat caaaacgatc ggttaaatgc
     1141 caggtcaggt cactgtatgc ggccagcgtt tcagcggtgg tatagccggt actgcttaaa
     1201 taaggcattg tcggcatgtc gtaggctgaa tttaacttct cgcgggtgtt ctgccggtac
     1261 agcccaaaca ccatatcaac ggtacgcgca tcgcccaggg ttgcggcgcg cagctcctgc
     1321 acatcctgat tccagcgctg aggcatattg acgattaacg aaccggaagg gaaggtgcgc
     1381 gaataatgct gctgctgcca ggcgctgatc aggttgaaaa cccagtcatc ggtggtgtat
     1441 ttcccactca gggtttggct gtcagtgcag cgccgcatgt acgggtctgg tgaaccatcg
     1501 ctgatcgaca gcttacggcc cttaatatca ttccatccca cataggcatc ctgggtggcg
     1561 cgagtacatt cgcgtgaggc ggcaaagccc atttcccagg gctgatcgtc cggcgccaga
     1621 cgcagtttca cattccctat gctggcgcgg gtgccgccta agtcatcgct tcccgtcgcg
     1681 gggttaatca tgtcgccgtc atcaacctgg cgtaacaggg tgacgctgcc gtacagcagg
     1741 ccatcctcaa tggggccgct caggttgaac ttacttcgat aactgtcgcg gctactgacg
     1801 ccgccttcaa tatagccgcg cggcgtgctg tccggctgct gggtgacgat gttgatgatc
     1861 ccgccctgag cgcttttgcc atataacgtt ccctgtgggc ctcgcagcaa ctccacgctt
     1921 tgcacatcgg taagcgcctg gatggtgttg gtggaaagct gagggacgcc atcgacatac
     1981 agggtgacgg cggggttata gaagtcctgc gctgaagaga cgccgcgtag cgagatcgtc
     2041 gaaaaaagca tgttgccgct attttcaata ttgagcccgg gcaagactct ggggagtttg
     2101 tcgctggcgg tgacgccggc gtcgcttaat tccggcgcgc tgacaacagt agacgagacg
     2161 ttgttggctg atgccgagcg ggaagattgt ttactggcgg taaccaccag cgtgctttcg
     2221 tcttgctgtg aagtctgggc attagcaatg gcggggagca ataatccccc caaggccaga
     2281 ggataaagcc gtgtcatttt cattgttgtg agtccctgta agacggcgaa acgccaaaaa
     2341 taccgacaat ggtgaattga aaatgataac tatttctaat agtggtgcta cccgaaacgg
     2401 gtttatttca acctgtttcg ggtcggtttg cgcttattgg gcagaatggc gataacggtc
     2461 gacggcgaga gccgtcaggc gttttttgtc tatcttgccg acggcggtga gcggccagtg
     2521 gtccagaaac tcgatttggt caggaatttt ccacgcgctg agccccatac gggtcagttg
     2581 ttgacgcaac tgctgatagt cagttggcac ctgctgcgcg atgataaacg cgcaaatccg
     2641 ctcgccaagc agcgtgtccg gcgcggcgac caccgcgcaa tcttgcactt ccgctaaacg
     2701 cagcagtgcc gattccactt cagccgcggc tattttttct ccggcgcggt tgatctgctc
     2761 ttttatgcgt ccctcaacgt gcaggttccc cacctcatcc agcctgacat tgtcgcctgt
     2821 gcggtaaaac ccttgcgcgg taaaggcctg cgcgttgtgg gcaggggcgc ggtaatagcc
     2881 cgaaatggta taagggccgc gcgttaacaa ttgcccggtt tcgcccggcg cgacgtcgtt
     2941 ctcgtcttga tcaacgatgc agatttcatc cagaggggac aacgggcgcc cctggctgtg
     3001 gagaatggtg gcatgcggat cgtccagccg ggtaaaacag agcaggcctt ccgccatacc
     3061 gaaaacctgt tgcagggtac agtcaaaggt ggcgataacc tgctcagcaa gcgtcgggtc
     3121 gagccgagcg ccgcctgcct gaatgacgcg cagcgacgaa aggtcgctgt cttcccactc
     3181 cctggcctgc acccataatt gcgccagcgc cggaaccagg gcgacgtgag tcactctttc
     3241 ctgcgcgatt aaaggcatca cctcatcaca gctggcgctg tcggtcagca ccacttttcc
     3301 gccgcaggca agcgttccca gaataccggg gcaggccagc ggaaagttat gcgccaccgg
     3361 gaggacggcg agatacacgc tctgttggct gatgccgcac agttcagcag aagcgctgaa
     3421 gttatagcta tagtcggcat gtcggcgcgg gatgagtttg ggcgtgccgg ttgtgccgcc
     3481 tgagagcaac aacaacgcgg tggtggaaac atcaggctgt cgctcaccgt gaagggagaa
     3541 gagcggcgta aaatcgtcgc tcatggtctc tccagcgacc agaacatgac gcaagcaggc
     3601 gtgtttgtgt gccatctgtc gggccagctc tgcgtggttt tccccgtgaa taacgtaagc
     3661 gacgggttgc gccagctcaa tcagcgcgtc gatatccagc gcccgttgcg agggcatcgc
     3721 cagcacgggg ataacgccca gtcttaacaa ggcgaacagc agggtaacaa acgcgttgtc
     3781 gttgggaagc tgcacaatta catgctcccc cgaacgcaaa cccagtgatg acagacctgc
     3841 ggcaagttcg tcaacttgtg catcaagcgc gctgtaggtt aacgacccct ttgcatcaac
     3901 gacggcaatt cgcgaggcat aacgggccgc ccagtggcgc aactgttcgg caatgggtaa
     3961 gggatactgt tcaatcagag attcaaagga agaattcatg cgtcaccttt ctgaagtact
     4021 gggctgtttt tgccacgcgg tcattgcaga aaatgcgtgg ggaaaatggc ggacaatctg
     4081 cgtaaaaaag gaccgggctt gttgaatggg atagaagtga tcgccgtcaa tcaccaccgg
     4141 gcctgtaacg tggctcagcc actgacgcca ggcatcgacc tgctgccagg aggcttcgcg
     4201 atcgtggctg ccgcacaata acagcgcagg cgtgcgcagc ggcggacaga cgtcgggcga
     4261 gtcgtagtga tagctctcgg tggcgtaaaa atcagcgcgc agaagaggaa gaaacagcga
     4321 cattaattcc tggttttccc gcagttcagg agaacatccg ccaatgtcta tcagctcagc
     4381 gataaagtcg gcatcatcgc gatggctgag ctggcgttca gaatgcagat gcggggcatg
     4441 gcacccggaa ataatcagtc cttgtggcgc aagcccccgt tgctccagaa gtcggcaggt
     4501 ttcaaacgcc acctgcgccc ccatgctgtg cccggcgagt aaaagcggcg tgtcaggcga
     4561 tacggatgct tccagctcgt tcgccagcag tgccgccagt tgtgtaatgc ttctgagcgg
     4621 ttccagatgg cgaaggcgat cgcgccccgg ccaggtcacc agagaaagcg cgcaatcagc
     4681 cagttgctca gcttgccagt ggcgaaacgc gctactgctg ccgccagcga aagggcacat
     4741 gaccagatgc gcagtattgc cgttccgggc gggccacagc gggatgcaca ttgcagattg
     4801 cgtcacagcg cctccttatc atcatcgttg tagaaaccgg ctaaccggtc gtggtgcggc
     4861 ggcgtcagac gtctgatctc cgcattgccg gtcttacgta aaatctgctg ccataaacgt
     4921 gataacgcga tctgatgtac gctttggcag gcctctggtg gatgttcgcc cgccagattg
     4981 ctgcgcaact ggtgtagcaa ccagctgacg ccctccggcc cgacggtttc gcagcagtcg
     5041 cgccagctca ggggcgccgc gctgcgcgtc agacccggca gatcgcgcag gatctccggt
     5101 ctgcgccaga gactgtgggg gttctgctga tggtctgcga catacagact ggatgaccag
     5161 atgacggggc cgtagctcgc ctccagcgaa agatgacctt ccggccagcc cagcagcagg
     5221 cggtgcatga taaggctatg catatctgga tcgtcaggat cgagatagcg ctgaagcagc
     5281 aggcaggctt ctccctccgg ccagaagagt ctcagacagt gaaaatcgct aaagctgcca
     5341 accatgtcgc actccacggc ggcggcatca acgcccagag ccagcaacaa caaatccaga
     5401 gtggagtaga gcaactgtcg gctggtggtg gcgtggacta ccggcggcgt cttagccagg
     5461 cagcgacgca gctgctgcgc atcgcgaagc catgtgcgtc ccgcgcgcgt atgcggataa
     5521 aacgtgttta cccaatagca gcagccctgt tcctgcgcca gcgtttgtaa ggaactgatg
     5581 tcatccgggt ggaggggatg ctcctgaatg acgtgtacgc cgcgcgtcag aaagtgtctg
     5641 gcaagctgcg taccggttcc tccggcgacg gtcgaacgca cgacaataca ggcgatatcc
     5701 ggcatcctgg ttatctgttc cggcgaggta tacagcggaa tgccaaacgc atgagccagc
     5761 tctcttgaac gggcgcttcc ctgcgccagc aggccgacca gttccagccc ctccgggggc
     5821 tgcataaagg cattcaggta catttcgcca aatttggcgc ccacaatcag tacgcgttgt
     5881 tttggggagg cggacggcat cataacgtgt tctccggttg cgttgaggtt gcgcaaagcc
     5941 agcgcgtaat gtgttgcgca caagcctgaa cgtggggggc ttccatcatg atttgccagt
     6001 ggctggcttc aatcacagcg tcgtcggcgt tgtttatcca gccctgcaac tcggtttctg
     6061 ctggcgtcca gcgcgcgggt ctcccggcgg catacaccat gagacagggg accggaacgc
     6121 tttcgcccgg cgtatgctca gtcagtaaac gcagcagatg cgcgatgttg tcgagccacg
     6181 tttctgccgc ttgcagcgtc aggttttgcg acaccatacc ggccgtttta gcgaggctga
     6241 taaagtcggc aagctggctg tcgggcgtct gctggtcgaa atgttcgggc agtgccagag
     6301 gcgtttctcc ttcggctaac aggcgcagca gggccgcccg gttttcgcaa cagaaatcct
     6361 gtcggcacac gggatcgatt aacaccatcc gaacctgctt gcctttggcg tacaggcgct
     6421 gtgcggcgcc cgccgcaaga aacgcgccat acgaccaacc cgccagcaca taagggccgt
     6481 gaggctgctg acgacggatg cacccgacat actcatcgat catctggtcg agcgtagcaa
     6541 agcgctgcgg cgattttgct tgcaggccga aaacgcgcct gttcaacgcg ctagcgagcg
     6601 gcagccaggc gctgatatcg ccgtctgaag cgtggaagac aaacagggtt tcctcaccgt
     6661 cgccctggca aaggggtatc gggttgtcct ctcctgacgt agccgcctgt agatgggcgc
     6721 agaagtcgct cagcgtcgaa tggttgaaca gatcctgaag gttagccctg gcaatccctc
     6781 tccggtttag ctgcgcgacc atccttgtcg ccatcaggct gtcgccgccc agttcgaaaa
     6841 agtcgtgatg ccttgccacg gggcgagaca gcagcgattg ccagagttca gccacctgtt
     6901 tttccagcgc cgaattcccc ccggcaactg gcgtgggttt ggctggctca gtcggcgcat
     6961 cagacggggc atgcagcggc gtaaataacg cttctctttg ccggatctgt aaaatgggca
     7021 gaccggtgcc aaagcgctgc tgtaaatagc ggctcacggc ttttttatcc gggcgattta
     7081 cgccaggcga acgcgctacc agcagatgct ggcgcagcgg cgacgattcc tgcgcgggcc
     7141 acgccagctc gtttgcaaac ccggcctgaa cgagaacctc ctgccatgcg gagcgggtga
     7201 gcatcggttt ctcatcccgg cggcggaaat cgcggtatcc gcttaatccc tcaataaagc
     7261 ccacgctcgc cagctggaat acgctgtttc gctccgtcgc ttcaacgatc agcaaacgtc
     7321 cgccggcttt cagcaacagt tttaatctgc gcaacgtctg gacgacatgg ctggcgtcgt
     7381 ggagcacatt gacggcaacg atcaggtcgt aacccgcctc cgggtgggcg gtgaaatcca
     7441 gcggctggtt gatgtcgaac aaggcataag acacctgcga ttcatcatgg aaacgggctc
     7501 tggcgtcatt gaggaactgc gcggagacgt ccgtgaagtg gtacgactgc cgcgtggcct
     7561 tcagcaccgg cgcggtagtg gctgcggttc cggcgccaac ctccagaatc cgttctgcgc
     7621 tgcacaaggc ggcaatctgc gcggtatagc gattcagaca ggcgctggcg gggttgtcgc
     7681 gatacagcgc gtcggtaacg cgatgctgct cgttgaacag caattccagc ggagaacact
     7741 gcccggagaa gagggcgtcg tgccgggcga tgcaggtttc cagatactgc gccagcgcct
     7801 ggctccattg gctttgcggg cacgcttcct gaggctcagg aatctcgctg agcggaatgc
     7861 ggcagcgcca gctttcacct tcgcggatta accactctct ttccgtcaga cactgtagcc
     7921 actggcgcag cagacgctgg tgagacgctt gcgcggacag cgcctgtact attgcgctaa
     7981 agcgatgggc gatctccggc gtggtaaaca ggccgtgacg atttagcgta gccgcgatcc
     8041 cctgtagcgc acgcgcctcc agccagcgcc aggtcgcctc cagctcatca agctgcgcct
     8101 gctcaggcaa cggcagcgcc agttgggtaa gttcgtgctc atcaggcatc gccgctgatg
     8161 gcgaaggatc gtcggccatc acgatatcca gcgtcaacgc gccctgttcg gttatttccg
     8221 ctctggcctg ccggatgccg gggtattcga gcgtcgcctg ctcaatatcc cgcaggtcgt
     8281 agtccgcctg cgcgttcagc ggccagcgtc ggcggggaat gtgcccggcg atgccgctgg
     8341 cgagagtctt caggctttca ggggcggcga tgacggcttg caggatggcg caatagtcat
     8401 taaacatcgc ctcggcagcg ccgggttcca gcacgttgtc catgcagtac cagctaaaca
     8461 tcaactcgcc gtcgctctcc atgacctgat gatccagcca gacctgcggc gtttgcgtga
     8521 atacatagca gggttcgccg aacagatggc tcatcgcctg atcgatagtc atgccttcca
     8581 gcgtcatccc cagcatactg gtaaacacta ccggcatcag cggttgacgt tgtgatccgc
     8641 gcaggcggcc cagctcacgg atcacctcaa caccgttcat ttcactgtgc gccatatttt
     8701 gccagaggcg ctgttgggtc tgttgcatct gctcttgcaa cgtcactggc gctgagaagt
     8761 taaaatcaac cagcgtgacg gaggtaaaat caccaatcaa ctggttgatt tgcggatgga
     8821 tcggctggcg attgaagaac gtcaggttca gcgtaaatgt tgtggtacgg ctccagcgct
     8881 caagggtggc ggcaaacagc gtgagcagcg cggcagacgg tgtgacgcct tgctgctgcc
     8941 agcgctgttt cacggcctgc cattctgtct tgccgatcgt cgatttgaag gtggtgaagt
     9001 gtggcgtttc cgggggcgtc tcaaccatcg gcagctctgg cgctaagggc agttgcggca
     9061 gtttttcctg ccagtaatcc caggcatcgt gccatgccga tgtctttcgg cgcgcctgtt
     9121 cagccatcac ataatcacgg aaggtaatag cgagcggcgc cagcgtttca ccgcgccaga
     9181 cctgcgccag gtcgtccatc atgactttaa aactctgcac atcaaactgc aaaaggtcga
     9241 ggttcatatg cagacggtaa tggcaatcgt cgatttcgct gaccaccagc tcaaaaagag
     9301 gccactggtc ggcaggcaaa acgcgatagc tcagttcatg ccgccgtttt tccagcgcga
     9361 tgcgctgttc ttccggggaa agcgcgcgca gatcgtcacg cgggatgtga tactccggcg
     9421 ttgtcgccag gattcgctgc tgcccgtcgg catcaaccac catacgcaac atatcgtggc
     9481 gtgcgatgag ctggttccat gctttctcca gtatggctag atcgaactca tcgtggcgtt
     9541 tatcccactc aaacaggacg tgacaggcga cgccgccata gccaatgagg tgggttcgcc
     9601 ccagccagta ggcgtgctga atgggcgtca aagggaaggg cgcataacgc tcgtcggcgt
     9661 cgtgccgcaa cacttccggc tgcgaaacgg caggcgtcgc ctctggctta gaacagataa
     9721 gctgcgtgag tccatgagga gacagatcct gccacgcccg ttccgcattg atgcgtacgc
     9781 ccaggtagtg ctgaatatcg ctactgagtt caaggaagag cagcgagtcc atgccgagtt
     9841 gcaacagatc ctggtttgga tgcagtgacg ccggatcgct cagccttagc tgaaccgcaa
     9901 tccgcttttt cagccatgcc atcaccgctg tttcatcgct caggctgccg ttaaaggcgt
     9961 tgtcatcagc aggaggaatg accggcgttg cggctttttc tgtggcgctg atgttaaaca
    10021 gagcctgttg taacgggggc attttgtcgg taaaaacgcg catcgccagt cgccacgggg
    10081 cgccgcgcat caccgcctgt tccaggtgcc agcagccttc ggcatcgctt aacgcgccca
    10141 tacctcggct ggcgagcgtc gccagcattt ccggcgtggc cgcccgaccg ctttctcccc
    10201 atgcgcccca ggcgacagag agcgttttcg gcgcatcaag ggtggaaaac tgctgggcca
    10261 gcccgtccag gtagccgcag gacagcgcat gggcgctctg acccggcgcg ccgagggtgg
    10321 cggcagcgga agagtagaga ataagatagc gtccgtcgtg gttgcgcagg gtttgcaaca
    10381 gctggcttgc cgcctgcgct tttaccgcga aaacggcagc cagctggtgg tcatcaagct
    10441 cctgcaaggg cgcgtcagcc aatacgccag cggcatgaat cgctccggca atgccgccgt
    10501 tggccgccag atcgtcaaga accgtggcca gttgcccggc atcgcccaca tcacaacggc
    10561 agacgcgcgt ctgcccgccc tccacgtcgc gtagccatga ctcatccacg tgcgatgcca
    10621 gcagggcgat gcgtcgcgcc cctttttctc tgagccagtt cacggcaagg cggcctaatc
    10681 cgccaaacgc tccggtcacc agatgccagc ggctatcgcc ggtaaacacg tttgccggta
    10741 attcagctgc gcatcccgta ttgggcgcca gtgaagggag ccaaagggtg tcaccccgtg
    10801 cggcgagcca gcgctgtgat agtgagactg cgctcaaccc ttgatgcagc gtttcccacg
    10861 gggtgttttc ggcgagatcg atggcggcaa gcaaccgttc cggctgttcg ttggccgcga
    10921 cgcgaagcaa ggcccatagc gcgtgatggg atgcagagag tgcttcattt tcctcgactc
    10981 gccacgcgcg gcgagtaacc acaatcaatc cggctgcgct ggcggtgagc gctgctatca
    11041 ctttctccgc taaggccagc gtatcctcgc tctcctcaac aatcatcagt cgtgaagagg
    11101 cgtgcggatc gtgaatgatg ccgtattgcg ccagctcgtc gctgcgcgca agcgtaccgg
    11161 cgctgaagct aaaggtaagc ggatggtcaa tgctggcgac gttgaggggc gtccagcgcc
    11221 attgataatg cgtttgcggc gcaggaagag gcgcggcggg cgatagcggc agccactcgc
    11281 cagcaggatg acgggcttca acgcgcatct gaccgcggga aaaggcttcg ttttgccagc
    11341 ttaagcgaac cttccccagc cactccgggg cggcgacggg gggcggttcc acaggcagag
    11401 cgtcgccatg acgctgtgaa agcagacgcc aggcctcctg ccagcgggcg ttaaactgtt
    11461 cgggctgacc tgcgcaatca gaccagtcgg cgagataatc accgttatcc gtcagcgcct
    11521 gccccaacac gggttctgat ggcgcggtga atgttacggc gctaaccgcc tgaccgggca
    11581 gcgtggcgag aatgatatgt tcgctcatcc cggcggtcgg gctgccatcc tgcggtagcc
    11641 acgccacttt gctgaatccg gcgtggcggc actgttgttg ccactgagcg gtggtgagga
    11701 ataactcacc ttcgcgggcg tcgagatcct gtagcggaag aaccagcggg ccgaaaacga
    11761 agtcaaacag acgcattggc tgggtgattt cgcgcatcag caggcgcccg cccggcttga
    11821 gcagggggcg cagattatcg agcgtgcggc caatatggcg ggtggcgtga atcacgttcg
    11881 ctgccacgat aagatcgtaa gactgtgcct ggaaaccctg agactgcgcc tctttttcga
    11941 gatccagctc gctatacttc acaaaatcat agtcggcgaa tttctgctgg gcgcgacggg
    12001 tgaacagcgc cgagatatcg gtgaaatggt actccagtgc cggaacgccg ttgagttccg
    12061 gcagcagcca cgcggtggtg ccgccggttc cgccgccaac ttcaagaata cgcaacgact
    12121 ggcggggctg acgcgtctgg acaatgccgc gtaatacccc ggcggcgatt tggttgaaat
    12181 agcggccaaa gctgaattcc tgatacagca cttccacgcc gtcggaggcg ctttgcggga
    12241 agataatcgc caccggttct tccgcgccgc tcatcatttc atataaccga tcgccggcac
    12301 gggcgatggt gtcgggaata gcctgaaaac cttcacaata actggcaagt tccgtcagca
    12361 gtgattcccg ctgttgatgt tcaatggggc gggcgcggac gtatcgcccg tcggtgcagc
    12421 ggtaatcgcc gtcgaccacg cagttattca gcaggcgctg gagtagctgc tggtagcggg
    12481 gcagcagacg tccacggcgc atgatggtca tggcgtccac gccgttctca atggcatcgc
    12541 cggtacagcg ttgtaccagt tgatcgacgt agatggcgtg cagtcgcgtg gcgcactgtt
    12601 taagcgcttc caggcgaggg agatcgagcg ctgtcgcggc gcgactcgcc acctccagcc
    12661 cggcagacag cgctgcgtcg gcaggctcgc aggccgggga gacgcgctct ttccagtaac
    12721 gttcagtatc aaacggataa catggcgcag cgatacgttg tccatcgccc gccagcaggt
    12781 cggcccacgg tagggcgacg ccggcagcgt aaagctggag cagggcctga ttgaggacat
    12841 cgctcgcctc tttgttacgc cgggcgctgg ctatccagta tgcgttatcg cggtattcgc
    12901 gctgcccgca agcaaccaac tgggcatcgg gccccatctc cagaaaaacg cgggcgccga
    12961 gctgatgcgc cacctgaata ctctggataa aacgcaccgg ctggcgcatg tgtcggcgcc
    13021 agtaatccgc ctggttgagc gttgactcat caatgacgtc ggcggtgagc gtggaaataa
    13081 tcggtatttg ccccggctcc gcgtgcagtc ccgcgcaggc gtcctggaac cgatcgagta
    13141 tcggctccag taaagcggag tgcgccgcac cggttacgct caggcgacga tagttaatgt
    13201 catgctgcga gagcgtggcg caaaataccg cgagacgggc ttccggcccg gaaaataccg
    13261 tatgttgcgt accgttgttg gcggcgagat ccagctcaaa ctggcgagcc agcggcatca
    13321 gcgtgtcttc gtctgcaaat accgccacca tcgcgccgct tgcgcactgc tgcattagcg
    13381 cgccgcgccg acaaaccagt ggcatgacct gttcaatcgt atagtgtccg cagacaacgg
    13441 cagcggcaaa ttcaccgacg gaatgcccaa tggcgaagtc tggcttcagt ccttcagcac
    13501 gccagtgcgc cgccatcgcg atttcaaacg cgacaatcgc cggctgcgcc caggccatat
    13561 tgtccagctg cgccgaatcg gggttaaaca tcgcttcgcg cagtgatggc gtgagcattt
    13621 cgctacaggc ggaaaaacag cgatccagcg tgtcggcaaa cgccgttgag tgctggtaca
    13681 tcgtttggcc catagtgtgc cagtgcgagc cctggccggt aaacagccac acctgcttgc
    13741 cgctggcgcc gtggccgctg taaaccagcg cccccgattt ctcaccggcc caggcgctga
    13801 gcgcctcggc ggtttcacgg tttaatggcg ccgccaggcg gaaggggaga tcgagacggc
    13861 gcgcgtgcag ggctgtgaag gccagagagc tggcatccgc attctctctc agcgccccgg
    13921 cataatccgt cgccagccgc cgcaacgcgc tgtcgctggc ggcgctgagc agcagcgcgg
    13981 tactttttct gccgctatcc gtattgggga ggcgcgcgtt gagcgcgtcg ggcagcgagg
    14041 cgacgatcat atggcagttg gtgccgccaa taccaaagga ggagacgccc gcatagcgca
    14101 tttcgtcctg ccatgcctgt gccgacaccg gtatggtaaa ggggctctct tcaagtttca
    14161 gcgccgggtt gggggtgtga aaattcagta agggaggaat ttgcccgcga ctgactgcca
    14221 gaacggtttt cagcagtccg gcaatgcccg ccgcggtatc cagatggccc atgttacttt
    14281 tcacggaacc gagcgcacag cgctgatcct gcgggcgagg cgcatagacg ttgcgtaacg
    14341 cttcaatttc aatcgcgtcg cccagcggtg tgccggtgcc gtgggtttca atgtaaccta
    14401 cctgcctgtc gtcgatggcc gccagcatta acgcctcttc gatgactgcc tgttgccctg
    14461 cgacggaagg ggcggtatag ccgacctttc tgttgccgtc gttgttgacc gcgctggaga
    14521 ggatcaccga gataatcgga tcgcctgaca gcagcgcgtc tctcaggcga cgcagcacca
    14581 cgcagccgag accgttaccg gcccaggtgc cctcagccga ggcgtcaaag ggacgacagt
    14641 gaccatcagg agagaaaatc attccgggct ggtagcggta gcctgcctgc tgggggaaag
    14701 agagcgccac gccgccggca accgccatat cggattcgcc agcgcgcagg ctttcacagg
    14761 ccagatgcac ggcaaccagc gagctggagc aggcggtctg taccgataac gccgggccgt
    14821 gcaggttgag tttgtacgcg gcgcgggtgg caatatagtc tttatcattg cccatcagag
    14881 attgcagacc ttttacctgc gcgacttctg tcacgttcaa tgcttcgcga ccggggtagg
    14941 tactcatccg ggaagaggcg aaaacgccgg tcttatgggg gacggcgccg ggggcataac
    15001 cggcatgttc cagcgcatgc cagaccgcct gcaaaaacag gcgctgctgc gggtccatcg
    15061 actccgcttc ctgtcgcgaa tagccaaaca gggtggcgtc gaagcagtcg gcgttgtcta
    15121 acaccgaacc gatattgaca taatgaggat cgtcaatgat ggcggcatcc agaccgacgg
    15181 ccagaagctc ttcgcgcgta aagcgtcggc tgcattcacg accttccagc agattttgcc
    15241 agaacgtttc gccatccggc gattcaggaa aatggcaggc gtagccgata accgcgacag
    15301 gttcgcagtc cgggtagtgt tgagcgatcg atgcatcaat ggaatctgct gtcggcgcag
    15361 aagagaagcg caagttatcc ataatcggtc ccgtttccta agattcacaa agtcaatgga
    15421 acgccatgcg ttcgcgatgg cgttccgggg aaaatcagtt tgcttcgcgc tatatccgcc
    15481 gctgacgacg gcgaacaggg cgaggtcgtt gtgttttctc caccgtggcg acgccggcca
    15541 gataatcggc cagggcagca ggagaagggt gggtgaagag gtcaagcaac gtcagggtgg
    15601 aaaattcgtg acgttgtaac aaaacgtgca gttgcaccag attcagcgac gttgcgcccg
    15661 cctcaaagaa gttttctgcg ggcgtgacag actcgccaac cacctcgcgg aaggcgtcgc
    15721 aaatcaggct gacggtgctc tcgtccgccg caatgtgtgg ctgagagtgc tcagggcata
    15781 cgcgcggtga gcgctctgcg cattgccctg cgtgtttaac gggaggcacc agcgcggcga
    15841 gcggcagttg ccagccgctt tcatcctcgg ccattcggtt tagcaatgcg caatactgtt
    15901 caaactggcg ctccacctgc ccggcaggaa acagcgcggc gacaaaatcc cagttaaagc
    15961 gcagttcgcc ttcggattca taaatctggt gatcgagcca gacctgcggc gtctgggaga
    16021 tgccccagac cggtttgagc agattacgcc gggcgaggaa gttatcctgc tcaaagccca
    16081 gcgcgctggt aaagacgacg ggcatcggaa cggctggcac gttttgccgt tgcgccagtt
    16141 gacgcatcac gcggatggct gacacatcgc ggtggttgag gttctggctc agccgctgct
    16201 gtagtgactg cgcgctgtgc agccagcttt cgccgggatg ccagctcagc agcatcagcg
    16261 aggtgaaatc gcccagaatc tggttgattt gcgggtgcag cggtcgcctg tcgaaaagcg
    16321 taaggttaag cgtgaagtca ggctgtgcac tccatgcaga gagaaccgtt gaccacaccg
    16381 acaacagtac ggccgacggg gtgagatggg cgtcagccgc ccgttttttc agccgatgcc
    16441 agcgcgtgct gtccagtgcg ccgttcaggc gggcgaagcg cggtgtttca acctcctgag
    16501 gcaagcagcg cagcggcaac gcaggcgccg gaggaatatc atcaagctgc gcctgccacc
    16561 atgccagaga atctggattg ggcgactgta gcgagggttg ttgcagataa tccctgaagg
    16621 tgacgggcag cggcggaagc agctgttgcg ggtagcggta gccgtgctcc agctccgcca
    16681 gcaggatctg catgctcaga ccgtcaagca acaggttatc cagacacagc cacaggcggg
    16741 caggcatccc gtccacgtat ccgacctgga gatcgaatac cggccatact tcggggttga
    16801 gtacctgatg cgccagtttt tcgcgcaccc gcaacgcctc ttcaggcgta tggagggtgt
    16861 gtgcgggtat cacccagggg ggcgtctgtt cgagcacctg ttgctgtcca tcacgcacga
    16921 tggcgcgcag catatcgtgg cgggcgatta atcggttcca gaccgtctcc agccgggtga
    16981 ggtccagatc ggcaatttca aattcaacaa agaaatgtga gccgacgccg cccagggcaa
    17041 agcccggctg acgccccacc aggtaagcct gctgcacgtc ggtaagcgca aagggctggt
    17101 agcgatcttc aggggagtgg acgaatggtt gttcgaccgg gacgtcggtt ttccgcagcg
    17161 tggcggcaaa atccgccagc cggggatgat taaacaggtc gcttaattgc gcttcataac
    17221 ctgcctgatg aagttgcccg gtcagacggg tcgccagcag gctatcgccg ccttgctgga
    17281 agaagtcggt ttctctggtg acattgccag ttgataagag ttgctgccag agggcggcaa
    17341 cctgtttttc aatgtcgccc tggggtaaat ccgcttcggc cgggttttcc gcttcagggg
    17401 tatgacgacg cttcagcgcc tggtagtcaa ttttcccgtt agctgtcagc ggtaaggcgt
    17461 cgaggaagac gatccgttgc ggcaccatcc accccggcag acgcccggcg agggcggcct
    17521 gaagttgacg gggatcgcgg cgcacctgcc tgtcaggaca ttgtacgagg aagcgttgta
    17581 accccgcgac ctcatcgcca tgctgacagt tgaagccggc cgcgctaaat aatgccgccc
    17641 agtcggcgct gttatgcagc aaggcctccg gctgcccatt ggttaacagg agcgtgctga
    17701 gtagggcgga cggcgttaac tggcgaaact ccatcacgta gagcagcgcg ccgggaacgg
    17761 caagctgttg taatgtcgca aggagcccgg gatcttccgg cagcagacga tgcagggcgt
    17821 tattaagcca gataatgtcc gccgagtgag cgtgcgccgc cagcgtgtct gcattccaga
    17881 gggacagacg ggcgccaggc cagggggcga gcctctgccg ggcgctcagc agcatctcct
    17941 ggctctgctc aagcccgaca tactcaatct gtccggcgtt gagctgtgct aacagcgatt
    18001 ctgcggcgcg gccagtgcgg gttcccacct caagcaggcg taccgggcgc tgtaagcgct
    18061 gtgcaagctg ttggcaaatt gtcgccagtt cctgaatata cggggcgctg gccggatggt
    18121 taaaggccag gctttccggc gaccactgcg cgtcgttcag taacgacagt tcgttacggg
    18181 caccgcgaag aatttgcgat aacgttaaat catgtccgct gaagtgcggg tgctcatcct
    18241 cgcgttcttc accagcgcag acctgataac cttcagcagc gggcttcagt cgccgttgtg
    18301 tcaccagaaa tgctaaccag cgttccacca cggcctgcca gcgcggctgg atagcgagtg
    18361 agttcatcag ggggagagga tcggcgccag cggtgtgacc cggcttcagt tttagcaggc
    18421 gatgctgaag gaaatcggct acctgttctg cggagatctc tggcgagatg gcgcaacagg
    18481 gcaacgttcc cgcaagcgtg tgccacgcct gcggcagtgc cgggttccga tgatcggtaa
    18541 cgcaaaaagc ctcgccctga ggaacaacgt atgccgccag cgttttttct ttttcgccga
    18601 tcgccagaac ggttgcttgt ttcaccccca ccagctggct gagcgcgctt tcgatttcgc
    18661 ccagctcgat gcgatatcct ccgactttga cctgcttgtc gcgacgaccg aggaactcga
    18721 ttgtgccatc tggccagtag cagccgagat cgccggtgcg ataccagcgc tcgtccggga
    18781 gcgtcaaaaa ttgctgctcg ctacgcaggg gatcgttgaa atagccttcc gcgaccccaa
    18841 tgccgccaat ccataattca cccggcaccc agtcagggca gtcccggccc tgttcatcca
    18901 ccacccggta gcgttggttg gttagcggaa aaccgtaagg gatggagcgc cagtaggcgg
    18961 ggacgtcgtg aatttcgcag gcgttagacc agatagacgc ctcggtggcg ccgcccatcg
    19021 cgataaattg tccttgtggc cggaaggccc gataacgggc ggggaggtca agcccgatcc
    19081 agtcgccgga aagcatcact gcgcgcaggt tttccggcgt ggcgtcggcg aaaccttcac
    19141 accaggtcag cagcatatcg aacagcgccg ggacgctgtt ccagagcgtg acctgatggc
    19201 gctggatcag ctcacaccat gcgtgaggat cgcgccgttg attttccatc accatcacca
    19261 gcgcgccgcc cgcgcgcagt acgccaaaaa tgtcgtaaac cgataaatca aaatgtaggg
    19321 cggagagggc cagcaccctg tcatgcgggc caacctgata gcgggtattg atatcgcaac
    19381 aggtgttaag cgctccccgg tgagaaatga ctaccccttt cggcgtaccg gtagagccgg
    19441 aggtgtagat aatgtaggcc ggttgcgtgg gggcgcgtac taccgggttg gcgatcggct
    19501 ccgcctcaat ggcctgctgc caggcaaggg cgggaatatc gtctgacccg gcgctggcgt
    19561 cgtgctgaca aatgagcacc agccggacgc tggcgtcagc gtagattttc tcgcgccgtg
    19621 cggcaggctg atccagcgaa accggaacgt aaaccgcccc ggccagcagg acggccagaa
    19681 ccgcaacaag ttgtcctgcg cctttcgaca tcgtgatagc cacattatcg ccgggctgaa
    19741 ccccgcactc gattaacctg cccgcgcaac ggcgggcata gtctgtcagc tcatgataat
    19801 tccactgata acgcatgtcc gttaccgcca gcgcctgcgg ctgttgcaga gcgatacgga
    19861 aaatgccttc atgcagcaag ccttcgggaa tgggggcgcc ggtggcgttg acccgttcgc
    19921 gtatcgcgcg ctggctggcg ggcatcatat ctgcgaacgg cttttgccag gcgctttcgt
    19981 catcgcagag ttggttaatc aactggcagt aggcgtcgaa caatgtttcg actaacgccg
    20041 gagggaacag cgcgtcgttg ctgtcccatt gtagccagac ctcgccgtga tgctcgaacg
    20101 ccagatgatc tatccagacc tgcggcgttt gcgagatgcc ccattccggc tcgcccaacg
    20161 gcgattctgc gcggctgctg tagagggaac gccccagatt gctggtaaat accaccgggg
    20221 cgccgtgggg gtagcgctgc tggcgtttga gttcacggag taattcgacg ccggaccagt
    20281 ggcgatgctc ccagtcctcc gtaaacgtga gctggttttt acgcgccagg ttgctgacgg
    20341 tatcgccatc gcaggcggta tccagcagaa gaatattggt gaagtcggca agcatcgcgc
    20401 caaccgccgg ttgcagcggc tggcggtcga ataaggtgat gttaagcagc agacgcgtca
    20461 ggccgcccca gcgagccagc acggcagaaa aacaggtcgc cagtgccatt gtcggcgtca
    20521 cgccatactc gccggcccgg ttgctaaagg cgtgccagcg tgttgccggg acaatcatgc
    20581 ggcgtcgggt attacggact tcacgtagcg tggcgggttc gcaggccagc ggcaagacgg
    20641 gcgcgggggg aagcgtcgat gctttcgcca gccagtaagc gcgcgcgacg tctctcagtg
    20701 gttgattgat cttctgctgg tgcagcaaat acgagcggaa atcatagcgg gtgtcgatag
    20761 ccggcagcga ttctccggcc agcagggcgt taagctcatc gaagaaaagc gtaaagctgg
    20821 aggcatccat aatcagcagg tcaatattga catggaggcg gtggcgattg tccggcaaga
    20881 gcgtcagctg aaaatcaaac gtttcgccga tttccacgcg taaaagacgg tggctcaggc
    20941 gctggcgcag tgcgtccaga taggcctggc ggctttcagc gtcgttatgg cgtaaatcat
    21001 gaacggtgac gccgttccag taaggttgcg gtagccagac ctgctgcccg tcggggcgaa
    21061 aggcgatatg cagcattggg tggcgttgca gcaaggtcgt gatggcctgc tccagctgcg
    21121 acgccgtcag acaatggcct tcaaactcct gatacaggtg gcaacccacg ccgccaagcg
    21181 tctgccccgg catgcggccc gtcaggtagg cgtgctgtac tggcgtcaat gggaaggggg
    21241 tactttcggt catgttcggc caggatgatt cgtcgggcgg cgtttcttct tccgcgttct
    21301 ccggcgaccg gctgagcatt aactggttcc atgccgccag cgtgggggcg gcatacagct
    21361 cgcgaagggt aaggcggtag ccatttttac gaaaccagtg taaccatctc atcaatctta
    21421 tggaatccag accggcctgg atcaggttgc tctcttcatg taactgctgc ggcgttaaat
    21481 tcagttcctg tatgagccgc tcgcgtaatt gttggtaatc agccgcgtgg cggttgtccg
    21541 gtaacagcga atcctgagat ggtgcgccag aaatcattct tcctcctgat ggcacgtctt
    21601 accgaaaagc attactcata tgagaataat aattattaac aattggatgc tacccgaaac
    21661 gggtttattt atgcccgttt cgggttggta acaggcggaa agaacagagg cccggatggg
    21721 gtatccgggc ctctgtcagg gaggagttta ggggggcgcg accccggtta catcccgcgt
    21781 ttaaaggtcg aaggagttac gccaaactgt ttctggaagg cggcagagaa atggctggcg
    21841 ttggcatagc ccagatcggc cgccaccgtc atgacggagg tattgccgtt ggccaggcgt
    21901 cttctggctt cctgcattcg ttcagcctga aacaggccat agacgctgtt gttgaacaga
    21961 acgcggaagc ctcgcttaat tttgagaacg ctcagaccgg actctctggc cagctccgcg
    22021 atgcccggcg cttgcgtcag atcagccagc agcagatcct tcgcccgcag cagtttctgg
    22081 cgttccatcg ggttgaagtg gcaggagagg caactggcgt cttcgcgatg ttcatccagc
    22141 accagactga gcatgaccag actttgcccc tgcaaccata gcgtcgacgg cttttgttca
    22201 tgattgaagc cgtcgtgcat gcgcatcagc gcgtgacgca aggcctgagc cgttaagtgg
    22261 gtttcagcat tacaacgctg ctgctggcaa cagcagtggg agtcgatctt attcttaatg
    22321 accgcgctaa tatccggcac ccacagcgcg agggtttcag ggctgaagga gagggtaatg
    22381 ctctcgaatc ggccacaata ggaggcgctg ccgcgacagt ctggcgtatg ggtaatgtaa
    22441 tggcgattgc gcgcaagctc ccagtcggtg tggcgccgga gtccctgaat cgcaaagcgc
    22501 gatgtgcctt ccaggatgca cgagaagtta atcctgccca gatcgtcgtg tagctgcaac
    22561 gtctcaggcc gcgcaaaatg gcccctccag agcaaaatat ccataccttc ctgcaacgaa
    22621 cagcgcagca gttcacactg agcaggaata ttgccatcgt ttgcaggttt cccgaccacc
    22681 aactggtgaa tagagatttc agattgcgtt tgcggtgact ccgtcatgac ctggttatct
    22741 ccctgtggct tagaccaatg cgggtataaa tagaaccgaa tggggtagat gagttattga
    22801 taatggttat tattcacatt aattctcaga acgccaccct gaattgtcgt ttttggcgtc
    22861 cgtaaaaatg catcaggaaa ttcagttact cccatgtcat ctcaatcatc caatactgaa
    22921 tcgctgtccc gatttcctct ctggcaggtg attacaccgg ttcgcaggaa ggttatttta
    22981 gctatggcgc tggcggggct ggcggcatta accagcctgg gggcgctcct ttttctggtg
    23041 tggagcctgc gtgacattcg cgcgacgcct gacgctattc cggcctggcc gctgggtggc
    23101 gtgatcggct gcgtggtctt aacttttgtt ctgcgcttac aggcgttcaa cacctctcat
    23161 tacgcggctt ttcatctgga gaacattctg cgcagcaggt tagcccgtaa agcattgcag
    23221 cttccgcctg gcgtgttaca gcaaatgggc agcgggtcag tggcgaaagt gatgctggat
    23281 gacgtgaagt cgttacatat ttttgtggcc gacagcacgc cgctctatgc ccgcgcgatc
    23341 atcatgccgc tggcgacaat cgttatcttg ttctggctgg actggcggct ggcaatcgcg
    23401 acgctggggg tactggcgtt tggatcggtt gttctcgtgc tcgcccgcca gcgttcggaa
    23461 gatatggctc agcgttatca taaggcgcgc gagcaggtta gcgcggcggt tattgagttc
    23521 gtgcaggcca tgcccgtggt gagaacgttt gatagcggca gcaccagttt tttgcgctat
    23581 caacgcgccc ttgaagagtg ggtcgatgtg ctccaaacct ggtatcgcaa agccggtttt
    23641 tcagcgcgtt tttccttctc gattctgaat cctctcccga ccctgtttgt cctgatctgg
    23701 tcgggatacg gcctgttgca ctatggcagt ttcgatttta tcgcgtgggt ggccgtttta
    23761 ctgctgggca gcggaatggc cgaagccgta atgccaatga tgatgctcaa taacctggtc
    23821 gcgcaaacgc gtttaagcat tcagcgtatt tatcaggttc tcgcgatgcc ggagttatcg
    23881 ctgccgcagt ctgagcagca gccgcaagag gcgagcatta cctttgagca ggtgagcttt
    23941 cattatccgc aagcgcgtac tggcgccgcg ttgcaggagg tgagctttca tgtgcctgcc
    24001 gggcaaattg tggcgctggt cgggccaagc ggcgccggaa aaagcaccgt ggcgcgcttg
    24061 ctgctgcgtt acgccgaccc ggacaaaggc catatccgta ttggcggcgt ggatctgcgt
    24121 gatatgcaga cggacaccct gatgaagcaa ctctcgtttg tgtttcagga caacttcctt
    24181 tttgtcgaca cgatagccaa taacattcgt ctgggcgcgc cggatacgcc gctggaggcg
    24241 gtaatagcgg cggccagagt ggcgcaggcc catgatttta ttagcgctct gccagaaggt
    24301 tacaacactc gagtcgggga acgtggggta tttctctccg gcggccagcg gcagcgcatt
    24361 actatcgccc gggcgctttt gcaggatcgc cccatcctgg tgctcgatga ggcgacggcg
    24421 tttgctgacc cggaaaacga agcggcgctt atcaaggcgc tcgcggcggc catgcgtggc
    24481 cggacggtca tcatggtcgc gcatcgtctc tcaatggtga ctcaggccga tgtgatattg
    24541 ctgttttccg acggacagct cagggaaatg gggaaccata cgcaactgtt ggcgcagggc
    24601 ggcctgtatc aacggctctg gcaacactat cagcaggcgc agcattgggt gccgggtgga
    24661 acacaggaag aggtggtgga aaatgaaaga caataatcct gcggataacc tggcctggcg
    24721 cgtcatctgg cgccagctta tctccagcgt tggcagtcag gccaggatgc tgcgacgcag
    24781 tatgctggcg ctgttgctgg cggcattcat gcaggggatc gcctttgcct gtctttatcc
    24841 gatcattgat gcgctgttac ggggagacgc gccgcaactt cttaactggg ctatggcctt
    24901 cagcgtcgcc gcaattgtga cgctggtgct acgctggtat ggcctgggct ttgaataccg
    24961 tggtcatctg gcgcaggcta cccatgagtt gcgcctgcga cttggcgagc agttacgccg
    25021 cgtgccgctg gagaagctcc agcgcggcag ggcgggtgaa atgaacgcct tgctgctggg
    25081 cagcgtggat gaaaacctca attatgttat tgcgatagcc aatattttgc tgctcaccat
    25141 tgtcacgccg ctgacggcgt cgctggcgac attgtggata gactggcggc tggggctggt
    25201 gatgttgctg atcttccctc tgctggtgcc gttttattac tggcgccgcc cggcgatgcg
    25261 gcgacaaatg cagacgctgg gggaagcgca ccagcgtctg agcggcgata tcgttgaatt
    25321 tgctcagggg atgatggtat tgcgcacctg cggcagcgat gccgataaaa gccgggcgct
    25381 gctggcgcat ttcaatgcgc tggaaaactt acagacccgc actcaccgtc agggcgctgg
    25441 cgcgacgatg ctgatcgcca gcgtcgtgga gttgggccta caggtggtgg tgttatccgg
    25501 gatcgtctgg gtagtgacgg gcactctgaa cctcgccttt ttgattgccg ccgtcgcgat
    25561 gattatgcgc ttcgcagaac cgatggcgat gtttatcagc tacacctcgg ttgtggaact
    25621 gatcgccagc gccctgcaac ggattgagca gtttatggcg atagcaccgc ttcccgtcgc
    25681 agagcaaagc gagatgccgg aacgttacga tatccgcttt gacaacgtca gctatcgcta
    25741 cgaagaaggc gacggccacg cgcttaatca tgtttctttg acgttcccgg cagccagtat
    25801 gagcgcgctg gtgggtgcct ccggcgcagg caaaactacc gtcaccaaac tgttaatgcg
    25861 ctatgccgat ccgcagcaag ggcagatttc tattggcggc gtcgatattc gccgcctgac
    25921 gccggaacag ctcaatagcc tgatttctgt tgttttccag gatgtctggc tgtttgatga
    25981 cacgctgctg gcgaatatcc gtatcgcgtg cccacaggcg acgcggcagg aggtagaaga
    26041 ggccgcctgc gcggcgcagt gccttgagtt tatttcccgt cttccgcaag gctggctgac
    26101 gccaatggga gagatgggcg gccagctatc gggcggcgag cgccagcgga tttccattgc
    26161 cagagcgtta ttgaaaaacg cgccagtcgt cattctcgat gaaccgactg ccgcgctgga
    26221 tattgaaagc gagctggcgg tgcaaaaagc gatcgataac ctggtttaca accggacggt
    26281 gattatcatc gctcaccgtt tatccaccat cgccggggcc ggaaacattc tggtgatgga
    26341 agagggacag gtggttgagc agggtactca tgcgcaattg ctctcacatc atgggcgtta
    26401 tcaggcgctg tggcaggcgc aaatggccgc gcgcgtgtgg cgcgacgacg gggtttccgc
    26461 gtctggagag tgggtgcatg agtgatgttc agtcgaatgt gaaaccgctg acgttgacga
    26521 ccgggcgggt gatttttgct attgccggcg tctatgtgac gcagagtctg gtatcggcgc
    26581 tgtctatgca gtccttaccc gcgctggtgc gcgctgctgg cggttcgctg gcgcttgccg
    26641 gtgcgacaac cctgtttatg ctgccctggg cgctgaagtt tatttgggcg ccgtggatcg
    26701 agcgctggcg gcttccgccc ggtagccagg aacgccgttc ccgcatgtta atcctgcgtg
    26761 gtcaggtcgc gctagcggcg atcctgacta ttgccgcagc gattggctgg tttaggcgag
    26821 aagggggatt ccccgatacg caaatcgtcg cgttatttgt tctgtttatg gtggcaggca
    26881 cggtcgcctc caccattgat atcgccagcg acggcttttg tgtcgatcaa ctgacccgca
    26941 cgggttacgg ctggggaaac agcgtgcagg tcggcggcag ctatctggga atgatgtgcg
    27001 gcggcggggt gttcatgatg ctgtcggcgg catccggctg gcctgtcgcc atgctgatga
    27061 tggcggtgct gattatggcg ctgtcactcc cgctgtggcg cattacggag ccgacgcgaa
    27121 cagcgactat cctgcatgtt ccggcgttag gttatgcgct aaggaggaag caggcgcgcc
    27181 tgggcttact gctggtattg atgctgaatt caggcatgcg gtttgtgctg cctcttctgg
    27241 caccgctgtt gttggatcat gggttgagca tgtccgcatt gggcgcgttg ttcagcggcg
    27301 gcaatattgc agcgggcata gcaggaacgc tggctggcgg attgctgatg aaatacacct
    27361 cacccggcag agcgctgttg acggcttatg gcgtccaggg gatcgcgctg ctggcggtgg
    27421 tgatgacgct catgatggcg ccgggtcatc tgctgctgca gattctccag tgtctggtca
    27481 ttgtccagtc catttcgctg gcctgcgcgc tggtctgtct ttacgccacg ctgatgtcgc
    27541 tttcatcgcc tttgcaggcc ggtgtcgact tcaccctctt tcaatgtact gatgcggcaa
    27601 tcgccattct ggcaggcgtt atcggtggcg ttgttgctca acattttggc tatgcggcct
    27661 gcttcctgtt tgccggggca ttcacgttgc tggcggcgtg ggttgcttat atccggctgc
    27721 attcggcaag agaactgatg acaagcgcaa ttgattgatc cgcgaataac acagagaggt
    27781 aagggatgaa aatcagtgaa tttctacatc tggcgttacc agaggaacaa tggctaccga
    27841 cgatttctgg cgttttacgc cagttcgcag aagaagagtg ttatgtctat gagcgtcaac
    27901 cctgttggta tttaggcaaa gggtgccagg cacggctgca cattaatgcc gacggaacgc
    27961 aggcgacatt tattgatgat gccggggagc aaaaatgggc ggtggattcc attgccgact
    28021 gcgcgcgtcg ttttatggcg catcctcagg tgaaaggacg tcgggtatat ggacaggttg
    28081 ggttcaactt tgcggcgcat gcgcggggga ttgcctttaa cgccggggag tggccgctgc
    28141 tgacgttaac cgttccccgt gaagaactta tttttgaaaa gggaaatgtc accgtttatg
    28201 cggactccgc cgacgggtgc cgacgtttgt gcgagtgggt aaaagaggcc ggtacaacga
    28261 cgcagaacgc accactggcg gtggataccg ccctcaatgg tgaggcatat aaacaacagg
    28321 ttgcgcgcgc cgttgcggag atccgccgtg gcgagtatgt caaagtgatt gtctcgcgcg
    28381 ccattcccct gccatcgcgg attgatatgc ccgccacgct gttatacggg cggcaggcaa
    28441 acacgcctgt gcgctcgttt atgttccgtc aggaaggacg cgaagcgctg ggctttagcc
    28501 cggaactggt gatgtcagtg atgggcaata aagtggtcac tgaaccgctt gcgggcaccc
    28561 gcgatcgcat gggaaacccg gagcataata aggcgaaaga ggcagaactg ctgcacgaca
    28621 gtaaagaggt gcttgagcat atcctttctg tcaaagaagc tattgctgaa ctggaggctg
    28681 tttgccagcc gggcagcgtg gtggttgaag atttaatgtc ggttcgccag cgcggcagcg
    28741 ttcagcatct ggggtctggc gtgagcggtc agctttcgga aaacaaggat gcctgggatg
    28801 cgtttaccgt gctgtttccg tcgattaccg cctcaggtat ccctaaaaat gctgctctga
    28861 acgcgattat gcaaattgag aagacgccgc gagagcttta ttccggcgca atcctgctgc
    28921 tggacgatac gcgctttgat gcggcgctag ttctgcgttc cgtatttcag gatagccagc
    28981 gctgctggat acaggcgggg gcgggaatca tcgcgcaatc tacgccggaa cgcgaactga
    29041 cagaaacccg ggagaaatta gcgagcattg cgccctattt aatggtgtag gagtaactgt
    29101 tttctaacct cacaaccaga ctgtccaaag acaattgagt cgctctttga ttcctctgtg
    29161 actctattgt cttcttttgg gataaaatag gtgtttttcg tcatgaagtt gataaaagtc
    29221 tatatagaac cacctcctgt ggaggtggtg gtaatttgcc agagtgaggg attcgttagt
    29281 atactatggg ataaacttat ttttaccaat tgtataaggc ggcgcataag attctcggca
    29341 tgcttccaga taatccgacc accactgcat catcgctttg cgggcttcta ggtgctctgc
    29401 cttatgaata taagccatgc gcacggtatt gcgctcctga tgactcatct ggcgttctac
    29461 tgcgtccttt gcccataaac ccgattccat cagcgcactg catgccattg cccggaagcc
    29521 gtgaccgcag atatcctttt tcgtgtcgta acccatcacc cgcagtgcct tattgaccgt
    29581 gttttcacac attggcttat acgggttatg gtcgccgggg aagatcagtt cattattacc
    29641 ggtgatatcc ttaatctgtt tcagaatggc gatggactgt tctgagaggg ggacgatatg
    29701 cggcattcgc atttttgccc cgcggccgga ataacgcacg ccaataatgg gttctcgcgt
    29761 cgcgggtatc gtccagactc ggtttgtgaa atcaatctct gaccagcggg cgaaacgcag
    29821 ttcactggag cgaatgaaca catgcagcat cagcagaacg gcatgccggg tcagttcacg
    29881 gccctgatga tatgccccaa tacgttcaag cagttcaggc agccgctcca gcggcagggc
    29941 aggatagtgc cgtctgacag gaggtgtggt cacgccgcca aggtttgctg cagggttcgt
    30001 atcgattaac tcctgatgga ccgcatggcg cattatgtta ctcaggtgct gccgtgtgcg
    30061 ggacgcaacc tccagctttg agataccagt ggcgggagcc acccggcttg accagcagat
    30121 acagaccgtg agaatcggag actttaaagg gtttatcaga aggcttgagg gtgcggattt
    30181 ttgcgtcggt aaggaacata tgggggtcac tccattatcg aactaacctg atcccagatt
    30241 tgaccaccaa tttttcccga tgcatagggt aaaatcaaaa tgcaccgaga agagttttca
    30301 cactaacctg ttgaatcaac atcagataaa gatttgcaag gatgcatgaa aacagaaaat
    30361 tggctcctct gactggactc gaaccagtga catacggatt aacagtccgc cgttctaccg
    30421 actgaactac agaggaatcg tgtgaacgga gcgcatatta cttagcggta ccttgtctgt
    30481 caacactaaa tttcataagc catttcaatt ggttaattaa tctgcaaagt tgttaattaa
    30541 tgaacattcg tcgccgaaaa cgagtctgta tcattagcgt gatgcagtct ctgcaaagga
    30601 tcttgttgat aaaattgaca gaaacgttgc cacaatgaag ggaaacgagg agcaaaaagt
    30661 tctggggcgc taaagaaata ttcagaaagt acggcaaaac attcagcagg atcactggca
    30721 gcataagcat caatgctcgc tgcattctca ccaactaatt cgatttcttc ctgaatgttg
    30781 ttcattgcag catgaagatc gtgttcccag ccagcaacct cacgcaacga aataaaggga
    30841 actccgctgg cgcgatcgcc gttacgggtg tccagcttat gagcgacttc atgaataatc
    30901 aggttaaaac cagatgcatc aaaagaatct tgtatatcca accagttcaa aacgataggc
    30961 ccttgctgcc agctctgacc tgactgaaca atacgttggt tatgcaccag accgatatcg
    31021 tcttcccatt catcatcgac cacaaatggc gcaggataaa ttaagacttc atgaaaacca
    31081 tccagccatt ccagtcctaa ctccagaacg ggtaggcaaa atagaagtgc tatccggcag
    31141 cttcttaatg aatccagttc aaagccctgt aaaggaacaa gccgcttttg ctgtaaaaaa
    31201 cgttcggcaa gagtgactaa tttgctttgt tcctgttccg tcagacccgt taaaaggggg
    31261 atcgatagtg cttcctgcca gggaagggca gtttgatgtg ctgattcttg tactttccag
    31321 ggccacttaa tcatcgtttt gctcgcaaac tcgtcacttg aacaaaattg cacggacagg
    31381 gactgttaaa atgccaaatt tcctggcatc atggcaacca tctgaacgga gagatgccgg
    31441 agcggctgaa cggaccggtc tcgaaaaccg gagtaggggc aactctaccg ggggttcaaa
    31501 tccccctctc tccgccactt tatcaatgac ttatctcctg acttcccgcc ttgcttttcc
    31561 taaacagaac aatcgtagaa tattcttgaa gggttagatc gtcactgttt tctgttcgat
    31621 actgtgacat tcagcacttg attcgctatg gagctgacag gaaggtttcg agcgaaaatc
    31681 tacagttatt cagtcgtttt cttatcggtc accattattc ttttagacat tgatcctatg
    31741 aaactgccgc aaagttgctg gcgggaactg aagttgcgta gagaaggggt caataattga
    31801 gtcaaaaaag gaacgcggcc gtcagattaa agccgcggga caaagtccat aatttccaag
    31861 agttgttgaa tttcccagct tttttgaaag cctttgtatc atactaacaa gtaaaaaagg
    31921 tttaaacacc aaaattaacc tatatttctg gttagcttta ccatgacaga tgacttgctc
    31981 ctggaaacat ttgcgctgcc agtctgtatg gctgagttgt gtatttattg ttcgcctttt
    32041 tcgcattttt tctacagaca taataaaaaa gacacgactc aagtatgagc gtgtctgagt
    32101 ttgtcttact tatttgtctt ggcaaattat tgttgttact accaggcact gttctgttaa
    32161 ataactgaag tcagcgcaaa aaaatcaatg agacatcatc tcctcgacca cttcttcgct
    32221 actcaactga tagggataat acgttggcca gttttccatt tcattcaata aagactgttg
    32281 ggaatcatta cccataaaaa tgtgaaaatg agatgatttt cgtggtgcaa ttatatgatc
    32341 gctaaattgt atatatttag gggctttact ttcaggatct ttacattcga ataagtaacg
    32401 aacgcctttc ttgcctgatt tataggtgag tattttgtat ccatcgtaat catatttaca
    32461 ggatgtt
//
LOCUS       DAZFDP010000035        32325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFDP010000035 DAZFDP010000000
VERSION     DAZFDP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHJ1G_001797"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHJ1G_001797"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4763666.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHJ1G_001798"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHJ1G_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4763667.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHJ1G_001799"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHJ1G_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4763668.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHJ1G_001800"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHJ1G_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4763669.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHJ1G_001801"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHJ1G_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO4763670.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..7380
                     /gene="bcsA"
                     /locus_tag="ACHJ1G_001802"
     CDS             4756..7380
                     /gene="bcsA"
                     /locus_tag="ACHJ1G_001802"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4763671.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPRPLDPVRYLIQTCWLLIGASRKETPK
                     PRRRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTF
                     SLILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWW
                     RYTSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPS
                     VDIFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIA
                     RTTHEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPH
                     HFFSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGG
                     IAVETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFR
                     LDNPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIAL
                     FVLPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAK
                     GGLVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLI
                     VLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQ
                     ILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTW
                     ALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPR
                     RPERSETAQPSDQALAQQ"
     gene            7391..9730
                     /gene="bcsB"
                     /locus_tag="ACHJ1G_001803"
     CDS             7391..9730
                     /gene="bcsB"
                     /locus_tag="ACHJ1G_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4763672.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9737..10843
                     /gene="bcsZ"
                     /locus_tag="ACHJ1G_001804"
     CDS             9737..10843
                     /gene="bcsZ"
                     /locus_tag="ACHJ1G_001804"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4763673.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10825..14298
                     /gene="bcsC"
                     /locus_tag="ACHJ1G_001805"
     CDS             10825..14298
                     /gene="bcsC"
                     /locus_tag="ACHJ1G_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO4763674.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14380..16368
                     /gene="hmsP"
                     /locus_tag="ACHJ1G_001806"
     CDS             14380..16368
                     /gene="hmsP"
                     /locus_tag="ACHJ1G_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4763675.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16551..17837
                     /gene="dctA"
                     /locus_tag="ACHJ1G_001807"
     CDS             16551..17837
                     /gene="dctA"
                     /locus_tag="ACHJ1G_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4763676.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18057..19553
                     /locus_tag="ACHJ1G_001808"
     CDS             18057..19553
                     /locus_tag="ACHJ1G_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4763677.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19649..20578)
                     /gene="kdgK"
                     /locus_tag="ACHJ1G_001809"
     CDS             complement(19649..20578)
                     /gene="kdgK"
                     /locus_tag="ACHJ1G_001809"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4763678.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20810..21577
                     /gene="pdeH"
                     /locus_tag="ACHJ1G_001810"
     CDS             20810..21577
                     /gene="pdeH"
                     /locus_tag="ACHJ1G_001810"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4763679.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21647..23707
                     /locus_tag="ACHJ1G_001811"
     CDS             21647..23707
                     /locus_tag="ACHJ1G_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4763680.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23941..25263)
                     /locus_tag="ACHJ1G_001812"
     CDS             complement(23941..25263)
                     /locus_tag="ACHJ1G_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763681.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25674..26687)
                     /gene="yhjD"
                     /locus_tag="ACHJ1G_001813"
     CDS             complement(25674..26687)
                     /gene="yhjD"
                     /locus_tag="ACHJ1G_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4763682.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26736..27635)
                     /locus_tag="ACHJ1G_001814"
     CDS             complement(26736..27635)
                     /locus_tag="ACHJ1G_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4763683.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            28155..28757
                     /locus_tag="ACHJ1G_001815"
     CDS             28155..28757
                     /locus_tag="ACHJ1G_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4763684.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28808..30457)
                     /locus_tag="ACHJ1G_001816"
     CDS             complement(28808..30457)
                     /locus_tag="ACHJ1G_001816"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4763685.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30862..32259
                     /gene="ccp"
                     /locus_tag="ACHJ1G_001817"
     CDS             30862..32259
                     /gene="ccp"
                     /locus_tag="ACHJ1G_001817"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO4763686.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         7694 a         8157 c         8874 g         7600 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggcta tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gttttgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcactcacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ctagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcattattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgttctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc actcgctggc
     4441 tatcgtcaat gtggatgcca actgccatat ccgcctgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaata acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattgct gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt cggtgaatac cgcagtgatg cgctggcggc
     4681 agaagagata ctgacgctgg cgaactggtg cctgttgaac tattccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgccacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc gcgtccgcgt
     4981 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca
     5041 tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca aaatattcgt
     5101 ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat
     5161 ctggatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt
     5221 atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc gtttaacccg
     5281 ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt acggcggatg
     5341 ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat
     5401 atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg
     5461 cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag
     5521 gtagtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc gctgtggccg
     5581 tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa aaatactatt
     5641 tacgcctcgc tgggtatcga ctggccgaaa gacaagctga acatctggat cctcgatgac
     5701 ggcggtaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgt
     5761 accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata tgccaaaggc
     5821 gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc
     5881 atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agacgccgca ccacttcttc
     5941 tcgccggacc cgtttgaacg caacctgggg cgttttcgta aaacgccgaa cgaaggcacg
     6001 ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt
     6061 tcctgtgcag taattcgtcg caaaccattg gatgaaattg gcggtattgc tgtcgaaacg
     6121 gtaaccgaag atgcgcacac gtctctgcgc ctgcaccgtc gtggctacac ctctgcatat
     6181 atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag
     6241 cgtattcgct gggcacgcgg gatggtgcaa atcttccgtc tcgataaccc gcttaccggt
     6301 aaagggctga aattcgccca gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg
     6361 ggcattccac gactgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac
     6421 atcatctatg cgccagcgtt gatgatcgcc ctgttcgtgc tgccgcatat gatccatgcc
     6481 agcctgacca actccaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac
     6541 gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac
     6601 aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg
     6661 gtgatctcgc ggccctacat cttccttgtc ctgctcaacc tggtgggcgt tgcggtaggc
     6721 atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg
     6781 tgggtattct acaacctgat tgttcttggc ggcgcagttg cagtatcggt agaaagcaaa
     6841 caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat
     6901 ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc
     6961 aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag
     7021 caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa
     7081 ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg
     7141 gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg
     7201 gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg
     7261 gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg
     7321 cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga
     7381 tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg ggatgagtgc
     7441 gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca atgctgagcc
     7501 agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc cgggcgtgca
     7561 gggcgctgat gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga agctgacctt
     7621 tgcgcaaatt gcgccgccgc cgggcagcat ggtgctacgt ggcattaacc cgaacggcag
     7681 cattgagttt ggcatgcgca gcgatgaagt ggtgacgaag gcgatgctca acctcgaata
     7741 cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga
     7801 gctgatgggc gtgctgccag tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat
     7861 gcccattaac ccactgttta ttaccgactt caaccgtgtg cggctggagt ttgtcggcca
     7921 ttatcaggac gtgtgcgaaa acccggccag caccacgctt tggctggatg ttgggcggag
     7981 cagtggactg gatctgacct atcagaccct gaatgtgaag aatgacctgt cacacttccc
     8041 ggtgccattc tttgacccgc gcgataaccg tactaacacc ttgccgatgg tctttgcggg
     8101 tgcgccggat gttgggctgc aacaagcctc tgccattgtc gcctcgtggt ttggttcgcg
     8161 ttctggctgg cgtgggcaga acttcccggt gctctataac caactgccgg atcgtaatgc
     8221 cattgtcttt gcaaccaacg acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa
     8281 agcccctgtg attgagatga ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt
     8341 gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa ggtatcgctc agggtaacat
     8401 tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc
     8461 gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc acatttggcg aactgaaaac
     8521 ctatgaagaa cagttacaat ccagcggtct tgagccagca gcgattaacg tttcgctaaa
     8581 cctgccgccg gatctctacc tgatgcgcag taccggcatt gatatggata ttaattaccg
     8641 ctacaccatg ccgccggtga aagacagttc gcggatggat atcagcctga ataaccagtt
     8701 cctgcaatcc ttcaatctga gcagcaaaca ggaggcgaac cgcctgctgc tgcggattcc
     8761 ggtattacaa ggtttgctgg atggcaaaac agatgtctct attccggcgc tgaaactggg
     8821 cgcgaccaac cagctgcgct tcgactttga gtatatgaac ccgatgccgg gcggttcggt
     8881 ggataactgt attaccttcc agccggtgca gaatcatgtg gtgattggtg acgactccac
     8941 catcgacttc tcgaagtatt accacttcat cccgatgccg gatctacgcg cctttgctaa
     9001 cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa
     9061 agcgcctaac gaagcacaga tggaaacgtt gctgaatact gttggtttta tcggcgcaca
     9121 gacgggcttc ccggcgatta atctgacggt gaccgatgat ggcagcacca ttcagggcaa
     9181 agatgccgac atcatgatca tcggtggtat cccggacaaa ctgaaagacg ataagcagat
     9241 cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt
     9301 ccccggcatt gtgccggacg agagcgatcg cgcggcagaa acccggtcaa cgctgacctc
     9361 ttccggtgcg atggcggcgg tgattggctt ccagtcgccg tataacgacc agcgcagcgt
     9421 gattgcgctg ttggcagata gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga
     9481 tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa
     9541 cagcctacgt gttggcgacg tttattacgt aggtcatctg ccgtggttcg agcgcgtgtg
     9601 gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg gcggctatca gtgtgatatt
     9661 gctggcatgg gtactgtggc gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga
     9721 taacgagtaa ttgaagatga atgtgttgcg tagtggaatc gtgacgatgc tgctgctggc
     9781 tgcctttagt gttcaggcag cctgtacctg gcctgcctgg gagcagttta aaaaggatta
     9841 catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca ccacctccga
     9901 agggcaaagt tacggcatgt tctttgccct ggcggctaac gaccgtgcag ctttcgataa
     9961 tattctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac gtttgcccgc
    10021 ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca attcggcctc
    10081 cgatggtgat atctggatgg cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca
    10141 gcgttatacc gacatcggca gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac
    10201 ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg ggttttgctg aggataacag
    10261 ctggcgtttt aaccccagct acctgccgcc gacgctggcg cagtatttca cccgctttgg
    10321 cgcgccgtgg accacgctgc gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa
    10381 aggtttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc taaaagccga
    10441 aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag gcatgatgcc
    10501 tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg cgacattcac
    10561 tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag cgcagggtaa
    10621 aggaccggtc ggtttttctg ccgccatgct gcccttttta caaaaccgcg atgcgcaggc
    10681 cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatgcctatt acaactatgt
    10741 gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga caaaaggtga
    10801 gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt cacgctttcc
    10861 ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca acagttgctg
    10921 gagcaagttc ggttaggcga agcgacccat cgtgaagatc tggtgcaaca gtcgttatat
    10981 cggctggaac ttattgatcc gaataacccg gacgtcgttg ccgcccgttt ccgttctttg
    11041 ttacgtcagg gcgatattga tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg
    11101 ccgagttcaa atgcgtataa atcgtcgcgg actacgatgc tactttccac gccggatggt
    11161 cgtcaggcac tgcaacaggc acgattgcag gcgacgaccg gtcatgcaga agaagctgtg
    11221 gcgagttaca acaaactgtt caacggtgcg ccgccggaag gtgacattgc tgtcgagtac
    11281 tggagtacgg tggcgaaaat tccggctcgc cgtggcgaag cgattaatca gttaaaacgc
    11341 atcaatgcgg atgcaccggg caatacgggc ctgcaaaaca atctggcgct attgctgttt
    11401 agtagcgatc gccgtgacga aggttttgcc gtcctggaac agatggcaaa atcgaacgcc
    11461 gggcgcgaag gggcctctaa aatctggtac gggcagatta aagacatgcc cgtcagtgat
    11521 gccagtgtgt cggcgctgaa aaaatatctc tcgatcttta gtgatggcga tagcgtggcg
    11581 gctgcgcaat cgcaactggc agaacagcaa aaacagctgg ccgatcctgc tttccgcgct
    11641 cgtgcgcaag gtttagcggc ggtggactct ggtatggcgg gtaaagccat tcccgaacta
    11701 caacaggcgg tgcgggcgaa cccgaaagac agtgaagctc tgggggcgct gggccaggcg
    11761 tattctcaga aaggcgatcg cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg
    11821 gacccgcaca gcagcaacaa cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg
    11881 ctggcgatcc agcagggcga tgctgcgctg aaagccaata atcctgaccg ggcagaacgc
    11941 ctgttccagc aggcgcgtaa tgtcgataac accgacagtt atgcagtgct ggggctgggc
    12001 gatgtggcga tggcgcgaaa agattatccc gccgccgaac gttattatca gcagaccttg
    12061 cgtatggaca gcggcaacac taacgccgtg cgcgggctgg caaatattta ccgccagcaa
    12121 tcgccagaaa aagctgaagc gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt
    12181 gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga ggcactggaa
    12241 aaccagggca aatgggcgca ggcggcagca cttcagcggc aacgactggc gctggacccc
    12301 ggcagcgtat ggattactta ccgactttcg caggatctct ggcaggccgg acaacgcagc
    12361 caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacgaccc ggagcaggtt
    12421 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
    12481 aacagcctgc cgcgcgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
    12541 agcgatcagg tgctggaaac cgctaatcgc ctgcgagaaa gcggcaaaga agctgaagcg
    12601 gaagcgatgc tgcgtcagca accaccttcc acgcgtattg acctcacgct ggctgactgg
    12661 gcgcagcagc gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgctg
    12721 gagccaacta acgccgatgc cattctgggg ctgacggaag tggatattgc tgccggtgac
    12781 acagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
    12841 acacagcggc gcgtggcgct ggcgcaggcg cagcttggag ataccgcagc ggcgcagcag
    12901 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
    12961 gtgctgcgcg acggtgcgaa gtttgaagcg caggcgggcg atccaaagca ggcgctggaa
    13021 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
    13081 acattcaccc gactgacccg taacgacgag aaagatgact ggctgaaacg cggcgtgcgc
    13141 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcaccctcga gcacgattac
    13201 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
    13261 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
    13321 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
    13381 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
    13441 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
    13501 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
    13561 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    13621 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    13681 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    13741 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    13801 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    13861 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    13921 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    13981 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    14041 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    14101 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    14161 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    14221 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    14281 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    14341 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    14401 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    14461 accgttttgc tgttccatct ggtccaacag aatcgctata acacggctac gcaactggaa
    14521 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    14581 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    14641 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    14701 gtaatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    14761 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    14821 atgtataagt tcgtgatgag caccctctca atgttagtga ccatttactt acttttgtcg
    14881 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    14941 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    15001 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    15061 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    15121 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    15181 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    15241 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    15301 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    15361 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcactat catgagcgag
    15421 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    15481 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    15541 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    15601 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    15661 tggttacagc cacaggtcga gatgaccagc ggaaaactgg tcagtgcgga agtgttactg
    15721 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    15781 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    15841 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    15901 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    15961 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    16021 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    16081 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    16141 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    16201 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    16261 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcgc
    16321 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    16381 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    16441 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    16501 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    16561 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    16621 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    16681 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    16741 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    16801 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    16861 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    16921 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    16981 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    17041 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    17101 tcatcttcgg catcatcaat atgatcatgc gtctggcgcc tattggtgcg ttcggggcaa
    17161 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    17221 tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    17281 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    17341 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    17401 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    17461 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    17521 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    17581 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    17641 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    17701 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    17761 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    17821 acgaattatc ctcttaatct catcacttat gcccgcactc ccttccccga gtgcgggtgt
    17881 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    17941 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    18001 agactgaccg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    18061 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    18121 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    18181 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    18241 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    18301 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    18361 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    18421 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    18481 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    18541 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    18601 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    18661 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    18721 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat aaacaaaacg tttggcgaac
    18781 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    18841 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    18901 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    18961 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    19021 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    19081 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    19141 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    19201 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    19261 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    19321 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    19381 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    19441 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    19501 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    19561 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    19621 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    19681 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    19741 cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    19801 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    19861 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    19921 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    19981 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    20041 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    20101 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    20161 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    20221 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    20281 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    20341 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    20401 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    20461 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    20521 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    20581 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    20641 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    20701 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    20761 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    20821 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    20881 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    20941 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    21001 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    21061 gattgatttg ctggcgcaaa aagccgactt ctttatagag cacggcctgc tggcatcggt
    21121 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    21181 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    21241 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    21301 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    21361 gtttgtgatg ctgcgtcagt cgccggaagg acgcacactc ttttctcagc ttttacatct
    21421 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacgccgg aagagtggcg
    21481 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    21541 gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgcctggct
    21601 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    21661 caaaataacc gctgcgattt cagtggcttt cttgttgttg attgtcgtgg cgatcatttt
    21721 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    21781 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    21841 agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag acatcattct
    21901 tggcaatcca ccggatattc ccgaagtcac gatggtgcat ttgccacgcg tagaggcaac
    21961 gctggccccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    22021 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatctcgc
    22081 caacgatgat aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    22141 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    22201 gatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    22261 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    22321 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    22381 agggacgccg tttccggtac aggctgattt ccgctctggt aacacccgtg ttgcttttga
    22441 tggcgtcgtg aatgatccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    22501 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    22561 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    22621 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    22681 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    22741 cctgggaccg ttaattggcg ttgactccgg gaaaggggca gaaaagtcga aacggtctga
    22801 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    22861 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    22921 gcatggcagt agcctgccga ttagtgatct ttctactcat atcatcctca aaaatgctga
    22981 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    23041 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    23101 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    23161 tgacgcggaa ctacgcggta gcggtaactc ggtggcggca cttttaggca acagtaacgg
    23221 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    23281 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gatgatgagg tgcgggtgaa
    23341 ctgcgcggcg gcgaatctgg atattgccaa cggcgtggcg cgcccgcaga tctttgcttt
    23401 cgatactgag aacgcgttga ttaacgttac cggcacggca agttttgctt ccgaacagct
    23461 ggatttgact attgatccgg agagtaaagg aattcggatt atcacactgc gttcgccgct
    23521 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccgggc cgttgattgc
    23581 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    23641 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    23701 gaagtgatga ggttggattg tgtttgctgg tgagttcggg agcagcgtgt tcccctcacc
    23761 ctaaccctct ccccaaaggg gcgaggggac tgttttgtgc gtgttgaaac aacgagtacc
    23821 gcttttccgt tcactttctg ggggagggct gttatgttcg cgatataaca tctcgctccg
    23881 cttttctccc tctccctttc agggagaggg ctggggtgag ggtggctgcc cacacgatac
    23941 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    24001 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    24061 gatatatggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga acgatgctcc
    24121 ggtgtaacgc acttctgtcg ggaacagctc tggtaacagc gcgcccatcg gcccgaaggt
    24181 cagtcccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    24241 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgattac
    24301 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    24361 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    24421 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    24481 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    24541 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    24601 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    24661 tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg aaagcagcaa
    24721 gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga aggagccata
    24781 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc cgccccattc
    24841 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg gggcgaaaat
    24901 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    24961 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    25021 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    25081 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    25141 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    25201 attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg ctgttgcttg
    25261 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    25321 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    25381 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    25441 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggaa
    25501 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    25561 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    25621 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    25681 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    25741 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    25801 aatgctgcgc cagaggggga tttcatcagc gatggcaagg tgtaggtcat cacgatttta
    25861 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    25921 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    25981 atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    26041 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgacag cgtcacaatc
    26101 agcgcaatta acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    26161 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    26221 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    26281 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagcgtcgga
    26341 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    26401 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    26461 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgca
    26521 cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    26581 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgttt aatcggctcg
    26641 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    26701 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    26761 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    26821 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    26881 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    26941 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    27001 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    27061 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    27121 agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg ggcgataacg
    27181 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    27241 accggacggt catggctact cagttccagc tcaattcccg gatgcagata gagaaattcc
    27301 gacaggcgcg gcagtaacag gcttttcgcg attcctggcg ggatgtcgat gcgtaattta
    27361 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    27421 acatcttttg ctcgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgtg
    27481 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    27541 cttccttttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    27601 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    27661 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    27721 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    27781 ctgagaaagg tgaatccccc taaacagagc gtatttttca gcgattccct ttaaagtgaa
    27841 tagaggacgt gttggctatt tgtgcttatt tttgtctcat tgccgtttgt tatttttgct
    27901 tgtggtcgcg gagtttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    27961 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    28021 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    28081 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    28141 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    28201 tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc agtcaggcag
    28261 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    28321 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    28381 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    28441 atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg gcggtaaata
    28501 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    28561 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    28621 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    28681 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    28741 taaatatctc ctcctgaaga ggataatttt acagccagca ggaagttagc ggctggctgt
    28801 aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg taccacaccg ttagtccagc
    28861 caaacccatc ctgcaacgga tactcgccac cgccgccttc gcggggaaca ccatcggcaa
    28921 tatggtattt ctcgatcatt ttgtgctgtt ccagatagaa ctgattcacc gttttcagcc
    28981 agctgcgcgc gatttcatca cccagaaggt catcgccata cattttaaat ccctgaattg
    29041 ccatccattg taacggtgcc cagccattgg gtttatccca ctgttcaccg gtttcgtact
    29101 cgcttgccag aatcccgcca ggtgtcagta accggctgcg tacggcgttt gccagacgat
    29161 cggcctgttc atggttcgcc atgccgacat agagcggcac aatggcggca gcggaaaaca
    29221 gcgccagttg ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca tcatcccaga
    29281 ggtaacggtt taccgcatcg cgacgggcac tggccttctg gcggaacagc gcttctgtct
    29341 ctttctcgcc tttcagcgcc gagatgttgg cgatggcgct ctccagttta aacaggaagg
    29401 cattcagatc gatggggatg aactgggtgg tacgaatgct cgccagacga ccagtatcac
    29461 gcagccaacg ggaagagtaa tcccaacctg aggccgctcc cgcgcgtaaa tcgcggtaca
    29521 cctcgttggg cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc caggattcgt
    29581 cacgcggcgt gtcgcgatcg tcccaataac ggttgagcag cgatccgtcc ggcatccgca
    29641 caacatggcg ataggcctga ttagggatca acgattctgc accgtccatc cagaaggcat
    29701 attccatttt aaggtggtcg agatagcggc gcgcaccgcg tacaccatct tcttcaaaca
    29761 actccaccat cagcgcaaaa accggtggtt gcgatcggct caaataatag gtgcggttgc
    29821 cgttggggat gtgaccatag ttttcgatca tccaggcgaa gttatcggcc atgcatttca
    29881 gcaaatcttc ccgaccactt tccgccagcc ccagcatggt gaaataggaa tcccaatagt
    29941 aggtttcgct aaaacggccg cccgggacaa tatatgactg cggtagcgcc agcagagaag
    30001 accacggaat gtgatcctgt ggttcgcggg ttagcaccgg ccacagctgg tcgatatgct
    30061 ctttcaggga attttgcggg tccgatacat actcgctgga gtagacctcc ggcagccaga
    30121 agtgattttc aacaaacttg cgcaagtcaa aatcacgatg acggcgcact ttacggtagc
    30181 ggattaaaat atccagcggg tccattttgg gtgcgcagtc gggaaaggtt ttactgtcgg
    30241 ggaaaatttt cgccgactga acatgctcga acagttcgag atagcgatcg gcaggcgtca
    30301 gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc cggttctgcc tcgatcatct
    30361 catccagttt taattcatac gggtccagtt catagcagag atcgacctcg atcatcagtt
    30421 cgtctggatt agggttttga attttctgat tgagcataag gatccaacct ccgaaattcg
    30481 tcgctaatta tcgggctttt ctttaagggt agtcattcgc tccgcctggt gggcgacaaa
    30541 gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa tatcaatgga
    30601 atttataaaa atacggattt cgtgttgttt ttcattttca gaacattcaa tcctgctctc
    30661 gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat cttattgata
    30721 gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg ttttttatga
    30781 tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt cacaataact
    30841 caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat cggcctggct
    30901 ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga taataaacgc
    30961 agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt tttaggcttt
    31021 ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc cgcctattat
    31081 tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata taaatctttt
    31141 aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca aagcgatctg
    31201 aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta taccgcgcta
    31261 cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg gattgcaaaa
    31321 cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa tgaaccggtg
    31381 cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg ctttgcgctg
    31441 tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg ccatgcgttg
    31501 aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc agttgggccg
    31561 attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg ggatggtcgt
    31621 gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga aatggcgtcg
    31681 aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa agcgcagttc
    31741 ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat tgctgaattt
    31801 gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg agatgaaaat
    31861 gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa atgtgcaact
    31921 tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct gaaaaaagac
    31981 tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac taaagaagag
    32041 cgtgataaat tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac ggcaccgtac
    32101 ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct gcgctatcag
    32161 gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct gcacagtctg
    32221 aacggggtgt acacgccgta tatgcaggat aaacaataat taatttgatc gcccgaacag
    32281 gaatgtttgg gcgattttta ttacgataat aaagtctgtt tttaa
//
LOCUS       DAZFDP010000036        31976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFDP010000036 DAZFDP010000000
VERSION     DAZFDP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..1055)
                     /gene="rarD"
                     /locus_tag="ACHJ1G_001818"
     CDS             complement(168..1055)
                     /gene="rarD"
                     /locus_tag="ACHJ1G_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4763687.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1107..1574)
                     /gene="yigI"
                     /locus_tag="ACHJ1G_001819"
     CDS             complement(1107..1574)
                     /gene="yigI"
                     /locus_tag="ACHJ1G_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4763688.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1739..2608
                     /gene="pldA"
                     /locus_tag="ACHJ1G_001820"
     CDS             1739..2608
                     /gene="pldA"
                     /locus_tag="ACHJ1G_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4763689.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2741..4570
                     /gene="recQ"
                     /locus_tag="ACHJ1G_001821"
     CDS             2741..4570
                     /gene="recQ"
                     /locus_tag="ACHJ1G_001821"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4763690.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4634..5254
                     /gene="rhtC"
                     /locus_tag="ACHJ1G_001822"
     CDS             4634..5254
                     /gene="rhtC"
                     /locus_tag="ACHJ1G_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4763691.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5316..5936)
                     /gene="rhtB"
                     /locus_tag="ACHJ1G_001823"
     CDS             complement(5316..5936)
                     /gene="rhtB"
                     /locus_tag="ACHJ1G_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4763692.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6047..7069
                     /gene="pldB"
                     /locus_tag="ACHJ1G_001824"
     CDS             6047..7069
                     /gene="pldB"
                     /locus_tag="ACHJ1G_001824"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4763693.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7077..7877
                     /gene="yigL"
                     /locus_tag="ACHJ1G_001825"
     CDS             7077..7877
                     /gene="yigL"
                     /locus_tag="ACHJ1G_001825"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4763694.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7953..8852
                     /gene="bioP"
                     /locus_tag="ACHJ1G_001826"
     CDS             7953..8852
                     /gene="bioP"
                     /locus_tag="ACHJ1G_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4763695.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8740..9693)
                     /gene="metR"
                     /locus_tag="ACHJ1G_001827"
     CDS             complement(8740..9693)
                     /gene="metR"
                     /locus_tag="ACHJ1G_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4763696.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9811..12072
                     /gene="metE"
                     /locus_tag="ACHJ1G_001828"
     CDS             9811..12072
                     /gene="metE"
                     /locus_tag="ACHJ1G_001828"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4763697.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12111..12926)
                     /gene="ysgA"
                     /locus_tag="ACHJ1G_001829"
     CDS             complement(12111..12926)
                     /gene="ysgA"
                     /locus_tag="ACHJ1G_001829"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4763698.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13188..13949
                     /gene="udp"
                     /locus_tag="ACHJ1G_001830"
     CDS             13188..13949
                     /gene="udp"
                     /locus_tag="ACHJ1G_001830"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4763699.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14090..15517
                     /gene="rmuC"
                     /locus_tag="ACHJ1G_001831"
     CDS             14090..15517
                     /gene="rmuC"
                     /locus_tag="ACHJ1G_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4763700.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15612..16367
                     /gene="ubiE"
                     /locus_tag="ACHJ1G_001832"
     CDS             15612..16367
                     /gene="ubiE"
                     /locus_tag="ACHJ1G_001832"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4763701.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16381..16986
                     /gene="ubiJ"
                     /locus_tag="ACHJ1G_001833"
     CDS             16381..16986
                     /gene="ubiJ"
                     /locus_tag="ACHJ1G_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4763702.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16983..18623
                     /gene="ubiB"
                     /locus_tag="ACHJ1G_001834"
     CDS             16983..18623
                     /gene="ubiB"
                     /locus_tag="ACHJ1G_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4763703.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18702..18971
                     /gene="tatA"
                     /locus_tag="ACHJ1G_001835"
     CDS             18702..18971
                     /gene="tatA"
                     /locus_tag="ACHJ1G_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4763704.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18975..19490
                     /gene="tatB"
                     /locus_tag="ACHJ1G_001836"
     CDS             18975..19490
                     /gene="tatB"
                     /locus_tag="ACHJ1G_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4763705.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19493..20269
                     /gene="tatC"
                     /locus_tag="ACHJ1G_001837"
     CDS             19493..20269
                     /gene="tatC"
                     /locus_tag="ACHJ1G_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4763706.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20311..21093
                     /gene="tatD"
                     /locus_tag="ACHJ1G_001838"
     CDS             20311..21093
                     /gene="tatD"
                     /locus_tag="ACHJ1G_001838"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4763707.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21090..21578)
                     /gene="rfaH"
                     /locus_tag="ACHJ1G_001839"
     CDS             complement(21090..21578)
                     /gene="rfaH"
                     /locus_tag="ACHJ1G_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4763708.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21745..23238
                     /gene="ubiD"
                     /locus_tag="ACHJ1G_001840"
     CDS             21745..23238
                     /gene="ubiD"
                     /locus_tag="ACHJ1G_001840"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4763709.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23284..23985
                     /gene="fre"
                     /locus_tag="ACHJ1G_001841"
     CDS             23284..23985
                     /gene="fre"
                     /locus_tag="ACHJ1G_001841"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4763710.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24177..25340)
                     /gene="fadA"
                     /locus_tag="ACHJ1G_001842"
     CDS             complement(24177..25340)
                     /gene="fadA"
                     /locus_tag="ACHJ1G_001842"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4763711.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25350..27539)
                     /gene="fadB"
                     /locus_tag="ACHJ1G_001843"
     CDS             complement(25350..27539)
                     /gene="fadB"
                     /locus_tag="ACHJ1G_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4763712.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27729..29060
                     /gene="pepQ"
                     /locus_tag="ACHJ1G_001844"
     CDS             27729..29060
                     /gene="pepQ"
                     /locus_tag="ACHJ1G_001844"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4763713.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29060..29674
                     /gene="yigZ"
                     /locus_tag="ACHJ1G_001845"
     CDS             29060..29674
                     /gene="yigZ"
                     /locus_tag="ACHJ1G_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4763714.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29713..31164
                     /gene="trkH"
                     /locus_tag="ACHJ1G_001846"
     CDS             29713..31164
                     /gene="trkH"
                     /locus_tag="ACHJ1G_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4763715.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31176..31721
                     /gene="hemG"
                     /locus_tag="ACHJ1G_001847"
     CDS             31176..31721
                     /gene="hemG"
                     /locus_tag="ACHJ1G_001847"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4763716.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7413 a         8157 c         8758 g         7648 t
ORIGIN      
        1 tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg gagttttggg
       61 ggtatttcct gtaacaaata ttgttctttt tcttatctta atttttttca aaaaaagata
      121 aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta actttttcta
      181 cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat aaaggcgaaa
      241 gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag caggaacatc
      301 agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaagcg cgtggcagcg
      361 gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag cagtaaattc
      421 agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc aaacaggtaa
      481 attgccgcca cgggcagcag ccacatggtt tcgattaaca tgccggtttg cgcttcaacg
      541 gcaatcttct tgcgtaccag accgtagaag gcaaaactaa atgccagtcc cagcgcgata
      601 ataggtagcg aaccaaaagt ccacagctgg actaacacgc cacatatcgc cagaatcacc
      661 gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag cacaatgttc
      721 accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt gttcaccgcc
      781 cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag cataaaaatt
      841 ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat gctcatcagc
      901 accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc ggcaggcacg
      961 tagtaaatca acttgaagta cgctggcgct ataccccaaa taaaataagc ggcaagagcg
     1021 agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt ttaattggta
     1081 acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt ggcactggca
     1141 atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt attgcctgca
     1201 cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag ataatcaacg
     1261 cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc actgatggtt
     1321 tcgtggcggg ttaaggtact tcccacgcac accagacctg cggcgacatc cagcgctgac
     1381 gcaataaccc cgccgtgcaa aatgctttgc gcccagttac ccaccatcat tggctgattt
     1441 ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gctccatccc taacgcgcgg
     1501 ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg ttcagcggtc
     1561 agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga ttttatgctt
     1621 ctttgttgtt ggtttctact ttaggaaggg ataactaacg gctatggagt taagtatgta
     1681 aaatagcccg cagaaaaata ttcaccttat caataattcg ttacggagaa cacgaccgat
     1741 gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag tatatgcaca
     1801 agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta ttatcgccaa
     1861 tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca actacctcat
     1921 ttacacccaa accagcgatc tgaataaaga agcgattgcc agttacgact gggcggaaaa
     1981 tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccgctgt ggcgtgggat
     2041 tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt ggcaactgtc
     2101 caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat tgttcctcgg
     2161 ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga tggggtataa
     2221 ccacgactct aacgggcgtt ccgacccgac ctcccgcagc tggaaccgcc tttatactcg
     2281 cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg tggtgggtaa
     2341 tactgacgat aacccggata tcaccaaata tatgggttac taccagctta aaatcggcta
     2401 tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac aactggaaca ccggctacgg
     2461 cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt atactcaggt
     2521 ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc gtgtcggtgt
     2581 gggggttatg ctaaacgatt tgttttgatg aacggttgag tggttggcaa atctggaacc
     2641 cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc gcaggcgctg
     2701 aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt
     2761 gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc aacagtttcg
     2821 ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc tggtcgtcat
     2881 gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc taaacggcct
     2941 taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc aactgcaagc
     3001 caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc aacttgaagt
     3061 gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg aacgcctgat
     3121 gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag ccgttgatga
     3181 agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg cgctcggtca
     3241 gttgcgccag cggttcccga cgctgccgtt tatggcgctg accgccacag ccgacgacac
     3301 cacgcgccag gatatcgtgc gcctgctggg gctgaacgat ccgctgattc aaatcagcag
     3361 ttttgaccgt ccgaatattc gctacatgct gatggagaag ttcaaaccgc tcgatcagtt
     3421 gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca acagccgggc
     3481 gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg cggcctatca
     3541 tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc gcgatgacct
     3601 gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa acgttcgctt
     3661 cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa ccggtcgcgc
     3721 cgggcgtgat ggcctgcccg cggaagcgat gctgttttac gatccggccg atatggcgtg
     3781 gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatatcg aacgtcataa
     3841 actcaatgcg atgggcgctt ttgccgaagc gcaaacttgc cgtcgtctgg tactgctgaa
     3901 ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc tcgatccgcc
     3961 gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg gtcgtgtgaa
     4021 tcagcgtttt ggtatgggct atgtggtgga agtgattcgc ggcgctaata accagcgtat
     4081 ccgcgactat ggtcatgaca aactaaaagt ctacggtatg ggccgtgata aaagtcatga
     4141 acattgggtg agcgtgatcc gccagctgat tcacctcggt ctggtgacgc aaaatattgc
     4201 ccagcattct gccctacaac tgacagaggc tgcgcgtccg gtgctgcgcg gcgaatcctc
     4261 tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt
     4321 cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta aatcgatagc
     4381 cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga ttgagatggc
     4441 tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg ggatgcgcaa
     4501 gctggaacgc tttggcaaac cgtttatggc gctgattcgt gcgcatgttg atggcgatga
     4561 cgaagagtag tcagcagcat aaaaaagtgc cagtatgaag actccgtaaa cgtttccccc
     4621 gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca ttgtggcgct
     4681 tatgagcccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc gttcccgtaa
     4741 agaagcgatg atgggcgtgc tgggcattac ctgcggcgta atggtttggg ctgggattgc
     4801 gctgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc tgattatggt
     4861 gggcggtggc ctgtatctct gctggatggg ttaccagatg ctacgcggtg cactgaaaaa
     4921 agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc gcagtttcct
     4981 gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg gctcggtgtt
     5041 ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt ttgcgctgat
     5101 cattgtcgaa acgctggcgt ggtttaccgt cgttgccagc ctgtttgccc tgccgcaaat
     5161 gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg cgttatttgc
     5221 cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc
     5281 ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc cgatgctaac
     5341 agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt catctgcttt
     5401 ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat catcacaata
     5461 atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg cggctgttgc
     5521 ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg attggtgaga
     5581 ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc cagcgattta
     5641 aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa gtaagccgcg
     5701 cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt ccccaacccc
     5761 acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat agacgccacc
     5821 gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc agagcctggc
     5881 gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa ggtcatgatg
     5941 aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc
     6001 acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt ttcagcagca
     6061 aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac cgctgactga
     6121 tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc cggtgcgctt
     6181 tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg ggcgtattga
     6241 gagctacgta aaatatgcgg aactggccta tgacctgttc catttggggt ttgatgtctt
     6301 aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc cgcatctcgg
     6361 gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc agcaggaggt
     6421 tcagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg gtgcgatctc
     6481 cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat
     6541 gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca actgggccga
     6601 agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc gcgcgttgcc
     6661 gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact tacgcttcta
     6721 tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac gcgaaagtat
     6781 tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa cgcttctctt
     6841 acaagctgaa gaggaacgcg tggtggataa ccgcatgcat gaccgttttt gtgaactccg
     6901 caccgccgcg ggccatcctg tcgaaggagg acggccgttg gtaattaaag gtgcttacca
     6961 tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca tcgttgattt
     7021 tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt
     7081 accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat acgttatccc
     7141 cttacgccaa agaaactctg aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga
     7201 ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatt aagtcttaca
     7261 tgattacctc caatggtgcg cgcgttcacg atctggatgg taatctgatt tttgctcata
     7321 acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat ccggacatca
     7381 ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc
     7441 gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta ctggagccgg
     7501 aaggcgtcag caaagtgttt ttcacctgcg attcccatga acaactgctg ccgctggagc
     7561 aggcgattaa cgctcgctgg ggcgatcgcg tcaacgtcag tttctctacc ttaacctgtc
     7621 tggaagtgat ggcgggcggc gtgtcgaaag gccatgcgct ggaagcagtg gcgaagaaac
     7681 tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgac gccgaaatgc
     7741 tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc
     7801 atcccgagct ggaagtgatt ggtactaatg ccgacgacgc ggtgccgcat tatctgcgta
     7861 aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata cttcgctaca
     7921 atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc accacgattc
     7981 tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc gatagctatt
     8041 ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc
     8101 gtggcaatag cctgaaaacg gtcggcctgt atatgttggt gggcgcgatg cagctcggcg
     8161 tgatgtatat gctgagtttc cgcgcgtatc tctacctgac ggtctccgaa ctgctgctgt
     8221 tcaccgtact gacgccgctc tacatcacgc tgatttatga catcatgagt aagcgccgtc
     8281 tgcgctgggg ctatgccttt agcgccttgt tggcggtgat tggtgccggg attattcgct
     8341 atgatcaggt caccgaccat ttctggactg gcttgctgct ggtgcaactc tccaatatca
     8401 cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg atgccacagc
     8461 ataacgcctt tgcgtggttc tatcttggcg cgtttctggt ggcggtaatt gcatggttct
     8521 tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt ctggtgtttc
     8581 ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgcg acgcaggtgg
     8641 acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg ctggtaaacc
     8701 tggctatctg gcaccaacag ccgcactggc caacgttcat tacaggcgcg ctggtgatcc
     8761 tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg
     8821 atcgcaggcg tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct gacgctgctc
     8881 gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg ttttggtcac
     8941 caccagaccc tggcgctcaa aactctctac tacccaatgc ggtagcgcgg caatacccat
     9001 ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca gtgacgggct
     9061 gacgcctgcc ggctgaagaa aatgccgcca gacatccagt cgactacgct gcaccggata
     9121 aatcaacagc gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg ccgccagtgg
     9181 atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat aatgcaggcc
     9241 actgcgcggc aggatatcgg aggtcatcac cagatccagc tcgccctgtt gcaacgcggg
     9301 ctgcgggtca aacgtcacgc ccgatttaaa atccatctct acctgcggcc agttcttatg
     9361 gaaattttct aacgcgggcg tcagccactg aatacagcta tggcactcaa tggcaatgcg
     9421 cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc tggctaattt gcggcagcac
     9481 ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg gctggctctt
     9541 acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt gagacagggc
     9601 ggattgcgtc tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc
     9661 ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg gcatgaataa
     9721 tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat ctggacggct
     9781 aaatactaaa aattccataa ggggcatata atgacaattc ttaatcacac cctcggtttc
     9841 cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg ggcggggaac
     9901 tccacgcgtg aagaactgct gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa
     9961 caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgtt
    10021 ctgaccacca gtctgctgct gggtaatgtt ccgccacgtc atcagaacaa agacggttcg
    10081 gtagatatcg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg
    10141 gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt gccggaattc
    10201 gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggaggaagt ggacgaggcg
    10261 ctggcgctgg gccacaacgt taagcctgtg ctgctggggc cggttactta cctgtggctg
    10321 gggaaagtga aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat tctgccggtt
    10381 tatcagcaag tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa
    10441 cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac
    10501 gcgctccagg gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat
    10561 ctcgacacga ttactgcgct gcctgttcag ggtctgcatg ttgacctcgt acatggtaaa
    10621 gatgacgttg ctgaactgca caagcgcctg ccttctgact ggttgctgtc tgcgggtctg
    10681 atcaatggtc gtaacgtctg gcgcgccgat cttaccgaga aatatgcgca aattaaggac
    10741 attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca cagccccatc
    10801 gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta
    10861 caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct
    10921 ctggcagagt ggagcgcccc gattcaggca cgtcgtcact ctacccgcgt acataatccg
    10981 gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat
    11041 gaagtgcgtg ccgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg
    11101 attggttctt tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc
    11161 aatctcgacg ccaataacta ccgcacgggc attgcggaac atatcaggca ggccattgtt
    11221 gaacaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg
    11281 gtggaatact ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag
    11341 agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgttag tcgcccggca
    11401 ccgattaccg tggagtgggc gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg
    11461 atgctgacgg ggccggtgac catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt
    11521 gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg aggtggccga tctggaagcg
    11581 gcagggattg gtatcatcca gattgacgaa ccggcgctgc gcgaaggttt accactgcgt
    11641 cgtagcgact gggacgccta tctccagtgg ggcgtagagg ccttccgtat caacgccgcc
    11701 gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc
    11761 atggattcca ttgcggcgct ggatgcagac gtcatcacca tcgaaacctc gcgttccgac
    11821 atggagttgc tggagtcgtt tgaagagttt gattatccga atgaaatcgg tccaggcgtc
    11881 tatgacattc actcgccaaa cgtaccgagc gtggaatgga ttgaagccct gctgaagaaa
    11941 gcggcaaaac gcattccggc agagcgcctg tgggtcaacc cagactgtgg cctgaaaacg
    12001 cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg
    12061 cgtctggggt aaatccaaac cgggtggtaa taccacccgg tcttttctca ttacagcgac
    12121 ttcttcccac catactgctt aaaccattcc agcatacgct gccagccatc tttcgcagat
    12181 tcggcatgat agctcgggcg ataatcagcg ttgaatgcat gcccggcatc cgggtagacg
    12241 ataatctctg ctttcgcatt agcagcccgc agcgcctggc gcatcgtttc aacgctctcc
    12301 tgcggaatgc tgttatcctg accgccatat aagccgagaa ccggcgcgtt aagatcggtt
    12361 gcgatatcaa caggttgttt cggtgaattc agcgacttat cgcccgtcag tttgccgtac
    12421 cacgccactg cggcttttag ctgtggatta tgcgcggcat acagccaggt gatacgtcca
    12481 ccccagcaga atccggtgat cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa
    12541 ctggcgacat gatcgagatc ggccagcacc tgcgagtcag gcacttttgc taccagaccg
    12601 ctaagcaacg tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca
    12661 ggtgcgatag ccagataccc ctccagcgcc agacggcgac aaatgtcgcg gatatgttca
    12721 tgcacgccaa aaatttcctg cactacaatg accactggca gtgggccatc gctttgcttt
    12781 ggtctggcat ggtaagcagg catgttatcc ccttgtgaag ggatagaggt gaagcccgcc
    12841 acaatcgcgt cgtccggggt ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca
    12901 aatccagatt gttgtgttgt tgccatggta ttctccgtac ccttataaaa atgttgcgca
    12961 atgttaacta tagtcagcat gcaacaaatc acattgcctg aatcggctca tcttttatgc
    13021 agtcctgcag aatgaagagt gatttatgtg atttgcatca cttttggtgg gtaaatttat
    13081 gcaacgcatt tgcgtcatgg tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag
    13141 tgtctttttg cttcttctga ctaaaccgat tcacagagga gttgtatatg tccaagtctg
    13201 atgtttttca tctcggcctc actaaaaacg atttacaagg ggctacgctt gccatcgtcc
    13261 ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg gttaagctgg
    13321 catctcaccg cgaattcacc acctggcgtg cagagctgga tggtaaacct gttatcgtct
    13381 gctctaccgg tatcggcggc ccgtctacct ctattgctgt tgaagagctg gcacagctgg
    13441 gcattcgcac cttcctgcgt atcggtacaa cgggcgctat tcagccgcat attaatgtgg
    13501 gtgatgtcct ggttaccacg gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac
    13561 cgctggaatt cccggctgtc gctgatttcg aatgtacgac tgcgctggtt gaagctgcga
    13621 aatccattgg cgcgacaact cacgttggcg tgacagcttc ttctgatacc ttctacccag
    13681 gtcaggaacg ttacgatact tactctggtc gcgtagttcg tcactttaaa ggttctatgg
    13741 aagagtggca ggcgatgggc gtaatgaact atgaaatgga atctgcaacc ctgctgacca
    13801 tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc
    13861 agcaagagat cccgaatgct gagacgatga aacaaaccga aagccatgcg gtgaaaatcg
    13921 tggtggaagc ggcgcgtcgt ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt
    13981 cggccttttg tatttttgcg tagcgcctcg caggaaatgc ctttccaact ggacgtttgt
    14041 acagcacaat tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc
    14101 aatcatggtt tacgcagtta ttgcgttggt gggtgtggca attggctggc tgtttgccag
    14161 ttatcaacat gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga
    14221 gttaagcgcg gcaaaacaac aaattaccca aagcgagcac tggcgtgcag agtgcgagtt
    14281 actcaataac gaagtgcgca gcctgcaaag tattaacacc tctctggagg ccgatctgcg
    14341 tgaagtaacc acgcggatgg aagccgcaca gcaacatgct gacgataaaa ttcgccagat
    14401 gattaacagc gagcagcgcc tcagtgagca gtttgaaaac ctcgccaacc gtatttttga
    14461 gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc
    14521 gctacgtgaa caactggacg gtttccgccg tcaggttcag gacagcttcg gtaaagaagc
    14581 acaagaacgc cataccctga cccacgaaat tcgcaatctc cagcaactca acgcgcaaat
    14641 ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa
    14701 ctggggcgag gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga
    14761 atatgaaacc caggtcagca tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat
    14821 cgtgcggctg ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta
    14881 tgaacgctat tttaacgccg aagacgacta cacccgcgaa agcgcgctac aggaacatat
    14941 cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg
    15001 gctgcgaact ctggattacg tgctgatgtt tattcccgtt gaacccgctt tttcactggc
    15061 gcttgaccgc cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag
    15121 cccgactacg ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt atgagcatca
    15181 aagccgcaac gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt
    15241 gttcatcgat gacatgtccg cgattggtca aagtctcgac aaagcgcagg ataactaccg
    15301 ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt
    15361 tcgcggttta ggagtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt
    15421 gagccaggat gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta
    15481 tcaacgcgat gatgaatata atcagcagtc gcgctagccc attaggggta gttaagccgg
    15541 gtagaaatct agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag
    15601 caggcattga gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg
    15661 cgaaggaaca aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg
    15721 atgtcatgaa tgatttgatg tcatttggta ttcatcgttt gtggaagcga ttcacgattg
    15781 attgcagcgg cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc
    15841 tgacagcgaa attctcccgc ctggtcggag aaactggcaa agtggtcctt gctgatatca
    15901 atgaatccat gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg
    15961 ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca
    16021 tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt
    16081 atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg
    16141 agccgctgag caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac
    16201 tggtcgcgaa cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg
    16261 atcaggatac cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc gactactaca
    16321 atctgacggc gggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa
    16381 atgcctttta aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat
    16441 cgctcacccg cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag
    16501 gtaaaaggct tttcgacgtc attgattctg gtgttcagcg aacgccaggt tgatgtactg
    16561 ggcgaatggg caggcgatgc tgactgcacc gttatcgcct acgccagtgt gttgccgaaa
    16621 cttcgcgatc gccagcagct taccgcactg attcgcagtg gtgagctgga agtgcagggc
    16681 gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg
    16741 gaactgctgg ccccttatac cggtgatatc gccgctgaag gaatcagcaa agccatgcgc
    16801 ggaggcgcaa agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc
    16861 attactgaag agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag
    16921 acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct
    16981 aaatgacgcc aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg
    17041 gacttgatga actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact
    17101 cattattctg gatgccaaat cggcataaag acaaactttt aggtgagcga ctacgactgg
    17161 ccctgcaaga actggggccg gtttggatca agttcgggca aatgttatca acccgccgcg
    17221 atctttttcc accgcatatt gccgatcagc tggcgttatt gcaggacaaa gttgctccgt
    17281 ttgatggcaa gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag
    17341 cgtggtttga cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata
    17401 ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc ccggatattt
    17461 tgccggtcat taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt
    17521 tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct
    17581 tgattgatga actgaatttg ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt
    17641 ttgaagacag cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga
    17701 tgatggtgat ggagcgcatt tacggcattc cggtgtctga tgttgcggcg ctggagaaaa
    17761 acggcactaa catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct
    17821 ttcgcgacag ctttttccat gccgatatgc accctggcaa catcttcgta agctatgaac
    17881 acccggaaaa cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag
    17941 aagataaacg ctatctggca gaaaacttta tcgccttctt taatcgcgac tatcgcaaag
    18001 tggcagagct acacgtcgat tcaggctggg tgccaccaga taccaacgtt gaagagttcg
    18061 aatttgccat tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt
    18121 ttggacatgt actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc
    18181 cgcaactggt gttactccag aaaaccctgc tctatgtcga aggggtagga cgccagcttt
    18241 atccgcagct cgatttatgg aaaacagcga agcctttcct ggagtcgtgg attaaagatc
    18301 aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa
    18361 aaatgccaga actgcctgaa ttggtttacg acagtttgcg ccagggcaag tatttacagc
    18421 acagtgttga taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc
    18481 gttattttct cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc
    18541 gacctgagtg ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg
    18601 tcggttggcg caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa
    18661 tgcggctttg tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt
    18721 ggcagttatt gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct
    18781 ccatcggttc cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg
    18841 aaccaaagca ggataaaacc agtcaggatg ctgattttac tgcgaaaact atcgccgata
    18901 agcaggcgga tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag
    18961 agcaggtgta atccgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg
    19021 gcctcgtcgt tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga
    19081 ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac
    19141 tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc
    19201 ccgaactgaa agcgtcgatg gatgaactac gccaggccgc ggagtcgatg aagcgttcct
    19261 acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg
    19321 tgaaagataa tgaagctgcg catgagggcg taacgcctgc cgctgcacaa acgcaggcca
    19381 gttcgccgga acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg
    19441 ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt
    19501 agaagatact caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg
    19561 cattatcgcg gtgatcgtga tattcctgtg tctggtctat ttcgccaatg atatctatca
    19621 cctggtatcc gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga
    19681 cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc
    19741 agcgccggtg attctctatc aggtgtgggc atttatcgcc ccagcgctgt ataagcatga
    19801 acgtcgcctg gtggtgccgc tgctggtttc cagctctctg ctgttttata tcggtatggc
    19861 attcgcctac tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga
    19921 aggggtgcag gtatccaccg acatcgccag ctatttaagc ttcgttatgg cgctgtttat
    19981 ggcgtttggt gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct ggatggggat
    20041 tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt
    20101 cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg ctattggcga tcccgatgta
    20161 ctgtctgttt gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaatcg
    20221 ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc
    20281 cgtcagggcg gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt
    20341 tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat
    20401 gggctactca tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt
    20461 cagtattcgt cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa
    20521 gctgcgactg aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt
    20581 gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg aagagcagga acgcgctttt
    20641 gttgctcagc tacgcattgc cgcagaatta aacatgccgg tatttatgca ctgtcgcgat
    20701 gcccacgagc ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt
    20761 cttcattgct ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat
    20821 atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg
    20881 ccgttgattc cggcggagaa attgctgatc gaaactgatg cgccgtatct gctcccgcgc
    20941 gatctcacgc caaagccatc atcccggcgc aacgagccag cccatctgcc ccatattttg
    21001 caacgtattg cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgct
    21061 aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttcttcaca
    21121 ctgtgcttaa tctctttatt aataagatta agcaatagca tggagcgagc ctcaccatcg
    21181 ggttcggtga aaatggcctg aaagccttcg aacgcgcctt cggtaataat caccttatca
    21241 cccggataag gggttgccgg atcgacaatg tctttcggtt tatataccga tagctgatga
    21301 atcaccgccg atgggactat cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg
    21361 cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat caaattcgac aaacaggtag
    21421 ttgggaaaca atggctcact gactgcagta cgttttccac gcacgatttt ttccagggtg
    21481 atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc acgttgaagt
    21541 tgcccgcgct tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc
    21601 ggggcgcaag gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat
    21661 accgtcagag ttcacgctaa tttaacaaat ttacagcatc gcaaagatga acgccgtata
    21721 atgggcgtag attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc
    21781 ttgacgctgc ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcat
    21841 ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc
    21901 gaaaacccta aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc
    21961 gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg
    22021 gcgtttctga aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg
    22081 cagtttaagc aagtattgaa catgccgaca aagcggctgc gtggtgcgcc ctgccaacaa
    22141 aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg
    22201 gaagatgccg cgccgctgat tacctggggg ctgacagtga cgcgcggccc gcataaagag
    22261 cggcagaatc tgggcattta tcgccagcag ctgattggta aaaacaaact gattatgcgc
    22321 tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc
    22381 gaacgtttcc cggtttctgt ggcgctgggt gccgatcccg ccacgattct cggtgcagtc
    22441 acccccgttc cggatacact ttcagagtat gcgtttgccg gattgctgcg cggtaccaag
    22501 accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg
    22561 ctggaagggt atatcgaaca aggtgaaacg gcgccggaag ggccgtatgg cgaccacacc
    22621 ggttactata acgaagtcga tagtttcccg gtatttaccg tgacgcatat tacccagcgt
    22681 gaagatgcga tttaccattc cacctatacc gggcgtccgc cagatgagcc cgcggtactg
    22741 ggcgtggcgt tgaacgaagt gtttgtaccg attctgcaaa aacagttccc ggaaattgtc
    22801 gatttttacc tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa
    22861 cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt
    22921 atgtacacta aatttgtgat cgtttgcgat gatgacgtca acgcacgcga ctggaacgat
    22981 gtgatttggg cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat
    23041 acgcctattg attatctgga ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg
    23101 ctggatgcca cgaataaatg gccgggggaa acccagcgtg aatggggacg tcccatcaaa
    23161 aaagatccag atgttgtcgc gcatattgac gccatctggg atgaactggc tatttttaac
    23221 aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag
    23281 cgcatgacaa ccttaagctg taaagtgacc tcggtagaag ctatcacgga taccgtatat
    23341 cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta
    23401 gtgatggatg agcgcgacaa acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg
    23461 tttatcgagc tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac
    23521 cgcatcctca aagatcatca aatcgtggtc gatattcccc acggagaagc gtggctgcgc
    23581 gatgatgaag agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc
    23641 tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc
    23701 gggcgtgaag agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttaaagcac
    23761 cctggtctgc aagtggtgcc ggtggttgaa caaccggaag caggctggcg tgggcgtact
    23821 ggtaccgtgt taacggcggt attgcaggat catggtacgc tggcagagca tgatatctat
    23881 attgccggac gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat
    23941 gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaaaccc
    24001 gcccctgaca ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg
    24061 tcgcatccgg caatcggtgc acgatgcctg atgcgacgct ggcgcgtctt atcaggccta
    24121 cattggtgca ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa
    24181 acccgctcaa acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca
    24241 aactgaacgt ctttgcgttc catcagattc agcagcgtgg tgctgatacg cgcaccggaa
    24301 cagcccagcg gatgacccag cgcgatcgca ccaccgttga ggttgatctt ctcgtcaatc
    24361 tgctcaatca gtcccagatc tttaatacat ggcaggatct gcgcggcaaa ggcttcgttc
    24421 atctcaaaca cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagcttcgag
    24481 gccggaaccg ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc
    24541 acacgagcgc gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg
    24601 gcagctgcgc catcggaaag tgcagaagat gtgcccgccg ttaccgtacc gtttactgga
    24661 tcaaacgccg gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg
    24721 tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac caccagttgg gatgatttca
    24781 tttttaaatg cggccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag
    24841 gcatcctgca tttcacggct gataccgtgc atacgcgcca gcatttctgc tgttaagccc
    24901 atcatgcccg ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga
    24961 ctcatcggca catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgccg
    25021 gtcataatca tccgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg
    25081 acggtaaccg ccgggacaga gtgtggtact tctgccagca gcgccgcgtt acgggcgatg
    25141 ttaaaaccct gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc
    25201 gcttccagcg ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttct
    25261 gcacgcacgt tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg
    25321 acaatgacaa cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg
    25381 gctggctcaa ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc
    25441 ggcacttcat acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg
    25501 ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg
    25561 tagaccagcg ccatatccgc ttccgccgga gtggcgataa tgccttcctc cagacagcgc
    25621 accacttcgt tgaccatcgg gatcatcatg cgggcgataa tctcctcttc gctgaaatcg
    25681 cgcttcggct ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc
    25741 ggcttacctt tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag
    25801 cggttggcat caaacagcgc gtcgatggca tcgcggtaat ctttctgcat cctctgcggg
    25861 aagcctgctg ccatgacagc ctgggcatga tgcgcggtgt caatgcccac gacatccagc
    25921 agatatgccg gacccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg
    25981 aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg
    26041 ttaacaaaga agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc
    26101 caggcgacaa ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc
    26161 aacggcattc ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgtttc
    26221 agcgcgttgg ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc
    26281 tggcgtactt tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca
    26341 accgcttcta ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatt
    26401 gtggagatca cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc
    26461 agcagtttcg cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg
    26521 acaaccggca cgcctttcca cgcagactgg taagcgatgc cgccgcccat aatgcctgca
    26581 cccagcaccg cggcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct
    26641 tttacatatt gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc
    26701 agcgggacaa aacttttgtt ttccaggttt aaggcttctt cacgaccaaa acgggccgca
    26761 gcttcaatgg tttttactgc ggtgatgggg gccggataat gtttccccgc tgtttgtgcg
    26821 accatccctt tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagtggt
    26881 tccagcttcg gctgacgttt tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa
    26941 accgcctttg cgccttcaac cagtttttct gctttgacta cgccatccac cagaccgatt
    27001 ttcagcgcct gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtct
    27061 gcgcccagca tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg
    27121 gtttccggca gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg
    27181 cattcgcagc caccgcccag cgcatagccg ttgacggcag caatggtcgg caccggcaga
    27241 tcttccaggc gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca
    27301 ggaacgagga acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg
    27361 ttcgaacgca gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg
    27421 ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc
    27481 agttcggcaa tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg
    27541 tcagtctcct gaatccgcaa ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt
    27601 gtgttaaaaa tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa
    27661 atcacgctct ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag
    27721 ggtaaaaaat ggaatcactg gcctcactct ataaaaatca tatagctacc ttacaggaac
    27781 ggactcgcga tgcgctggcg cgcttcaagc tggatgcgtt acttattcac tccggcgaac
    27841 tgttcaacgt ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag
    27901 cgtgggtgcc ggtaactcag gtgccaaact gctggctgct ggtggatggc gtgaacaagc
    27961 cgaaactgtg gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct
    28021 ctttctggac tgaagacgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc
    28081 tgctgcctgc tgcgcgcggg aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc
    28141 aactgggtat tgaggccagc aatatcaacc cgaaaggggt gatcgactac ctgcattact
    28201 accgctcctt caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg
    28261 tcaacggtca tcgcgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca
    28321 acattgccta tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg
    28381 tggcgctcaa cgaacacgct gcggtgttgc attacaccaa actggaccac caggcaccgg
    28441 aagagatgcg cagcttcctg ctggatgccg gggccgaata taacggctat gccgctgacc
    28501 tgacccgtac ctggtcggca aaaagtgaca acgattacgc acagctggtg aaagacgtaa
    28561 atgatgaaca actggcgctg atcgcgacca tgaaagctgg cgttagctat gtggattacc
    28621 acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata
    28681 tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc gtttatgccg cacggtatcg
    28741 gccatccgct gggcctgcag gtgcatgacg tcgccggttt tatgcaggat gatagcggta
    28801 cgcacctcgc ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg
    28861 gcatggtgtt aaccatcgaa ccgggtatct acttcattga atcgctactg gcaccgtggc
    28921 gtgaagggca gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg
    28981 gcggcattcg tatcgaagac aacgtggtga tccacgaaaa taacgtggaa aacatgaccc
    29041 gggatctgaa actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt
    29101 tgaagagatc aaaaagagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc
    29161 ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt
    29221 ggcgtgggtg gcgggtgcgc cggatgattc acaacagctg ggtttctctg acgacgggga
    29281 gccggcggga acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgttgggga
    29341 aattaccgct gtggtggtgc gctactacgg cggcatattg cttggcaccg gtgggttagt
    29401 gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac
    29461 gccattaacc gaatatactt tgcaatgtga atatcatcag ttaaccggca ttgaagcgtt
    29521 gctggggcag tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg
    29581 ggtggcgctt ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg
    29641 tggttcattg caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag
    29701 gaagcggcag agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta
    29761 ttttcaggga ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc
    29821 cgcgctttta cccagacctt ttttgtcgcc ctcgccattg gctctatgct gtggtggccg
    29881 aaccgcaaag agaaaggcga acttaaatcc cgtgaggggt ttctgatagt ggtgctgttc
    29941 tggaccgtgc tgggtagcgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc
    30001 acgattaccg atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg
    30061 ctggtggggc tggattcgct ccctcacgcc atcctctttt atcgccagat gctgcaatgg
    30121 tttggcggga tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt
    30181 gggatgcagc tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg
    30241 cgaattgcgg aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt
    30301 gcgctggcgt tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg
    30361 actatcgcta ttggcggctt ctcgacacat gatgccagta tcggttattt cgatagcccg
    30421 actattaaca ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac
    30481 ttttcactgt taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg
    30541 tttatcggcg tacagtttac gctggtggtt atttgtaccc tcgtactgtg gtttcataat
    30601 gtctacagtt cggcgctgat gacaattaac caggcgtttt tccaggtggt gtcgatggcg
    30661 acaaccgccg ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg
    30721 cttttatgtt cggcttttat cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg
    30781 atccgcatcc tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg
    30841 aacgccgtct atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc
    30901 gtttggggat ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc
    30961 gccacgggcg tggatgactt ttctgccttt gcctcagttg ttgcgacatt gaataacctg
    31021 gggccagggc ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg
    31081 atcctgattg ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt
    31141 accccgactt tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa
    31201 caagggacgg acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg
    31261 ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaattatg
    31321 accgtgtggt cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat
    31381 ttgtcaaaaa acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc
    31441 tggtggcgcg caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc
    31501 tgatgaactc gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc
    31561 cacgttatcg ctggtacgac cgttttatga tcaagctgat tatgaagatg tcaggcggtg
    31621 aaacggatac gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc
    31681 gagaaatcgc ccatttaacc gacaaaccga cgctgaaata agcacaaaga ataaaaaatg
    31741 cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc
    31801 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
    31861 attccgacag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
    31921 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcaga
//
LOCUS       DAZFDP010000037        31888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFDP010000037 DAZFDP010000000
VERSION     DAZFDP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..754
                     /locus_tag="ACHJ1G_001848"
     CDS             38..754
                     /locus_tag="ACHJ1G_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4763717.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            784..1284
                     /locus_tag="ACHJ1G_001849"
     CDS             784..1284
                     /locus_tag="ACHJ1G_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO4763718.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1376..4060
                     /locus_tag="ACHJ1G_001850"
     CDS             1376..4060
                     /locus_tag="ACHJ1G_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO4763719.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4057..5131
                     /locus_tag="ACHJ1G_001851"
                     /pseudo
     CDS             4057..5131
                     /locus_tag="ACHJ1G_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5219..6517)
                     /gene="exuT"
                     /locus_tag="ACHJ1G_001852"
     CDS             complement(5219..6517)
                     /gene="exuT"
                     /locus_tag="ACHJ1G_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4763720.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7000..8412
                     /gene="uxaC"
                     /locus_tag="ACHJ1G_001853"
     CDS             7000..8412
                     /gene="uxaC"
                     /locus_tag="ACHJ1G_001853"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4763721.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8427..9914
                     /gene="uxaA"
                     /locus_tag="ACHJ1G_001854"
     CDS             8427..9914
                     /gene="uxaA"
                     /locus_tag="ACHJ1G_001854"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4763722.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9997..10548
                     /gene="ygjV"
                     /locus_tag="ACHJ1G_001855"
     CDS             9997..10548
                     /gene="ygjV"
                     /locus_tag="ACHJ1G_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO4763723.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10553..11797)
                     /gene="sstT"
                     /locus_tag="ACHJ1G_001856"
     CDS             complement(10553..11797)
                     /gene="sstT"
                     /locus_tag="ACHJ1G_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4763724.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12196..13161)
                     /gene="alx"
                     /locus_tag="ACHJ1G_001857"
     CDS             complement(12196..13161)
                     /gene="alx"
                     /locus_tag="ACHJ1G_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4763725.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13444..14430)
                     /gene="ygjR"
                     /locus_tag="ACHJ1G_001858"
     CDS             complement(13444..14430)
                     /gene="ygjR"
                     /locus_tag="ACHJ1G_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4763726.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14509..15188)
                     /gene="ygjQ"
                     /locus_tag="ACHJ1G_001859"
                     /pseudo
     CDS             complement(14509..15188)
                     /gene="ygjQ"
                     /locus_tag="ACHJ1G_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15265..15768)
                     /gene="ygjP"
                     /locus_tag="ACHJ1G_001860"
     CDS             complement(15265..15768)
                     /gene="ygjP"
                     /locus_tag="ACHJ1G_001860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4763727.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15853..16989
                     /gene="rlmG"
                     /locus_tag="ACHJ1G_001861"
     CDS             15853..16989
                     /gene="rlmG"
                     /locus_tag="ACHJ1G_001861"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4763728.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17180..17263
                     /locus_tag="ACHJ1G_001862"
                     /pseudo
     CDS             17180..17263
                     /locus_tag="ACHJ1G_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17260..17676
                     /gene="higA"
                     /locus_tag="ACHJ1G_001863"
     CDS             17260..17676
                     /gene="higA"
                     /locus_tag="ACHJ1G_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO4763729.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17721..19472)
                     /gene="fadH"
                     /locus_tag="ACHJ1G_001864"
                     /pseudo
     CDS             complement(17721..19472)
                     /gene="fadH"
                     /locus_tag="ACHJ1G_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            complement(19577..20314)
                     /locus_tag="ACHJ1G_001865"
     CDS             complement(19577..20314)
                     /locus_tag="ACHJ1G_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4763730.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWMHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRSAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVHPYPKPQTVKTGLKESE"
     gene            complement(20268..20468)
                     /locus_tag="ACHJ1G_001866"
     CDS             complement(20268..20468)
                     /locus_tag="ACHJ1G_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4763731.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            20583..21045
                     /locus_tag="ACHJ1G_001867"
                     /pseudo
     CDS             20583..21045
                     /locus_tag="ACHJ1G_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21084..21329)
                     /locus_tag="ACHJ1G_001868"
     CDS             complement(21084..21329)
                     /locus_tag="ACHJ1G_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4763732.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(21365..21544)
                     /locus_tag="ACHJ1G_001869"
     CDS             complement(21365..21544)
                     /locus_tag="ACHJ1G_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4763733.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(21602..23659)
                     /locus_tag="ACHJ1G_001870"
     CDS             complement(21602..23659)
                     /locus_tag="ACHJ1G_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4763734.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTQDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVAPDGEAL
                     PQPAPGGKPEAPTGSQPGGSESPHPTSPSLRTLLSLKASAGEAPAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTHGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(23750..24310)
                     /locus_tag="ACHJ1G_001871"
     CDS             complement(23750..24310)
                     /locus_tag="ACHJ1G_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4763735.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(24324..24503)
                     /locus_tag="ACHJ1G_001872"
     CDS             complement(24324..24503)
                     /locus_tag="ACHJ1G_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763736.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISVLKGIFTSF"
     gene            24532..24735
                     /locus_tag="ACHJ1G_001873"
     CDS             24532..24735
                     /locus_tag="ACHJ1G_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4763737.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            24815..25336
                     /locus_tag="ACHJ1G_001874"
     CDS             24815..25336
                     /locus_tag="ACHJ1G_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4763738.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(25371..26282)
                     /locus_tag="ACHJ1G_001875"
     CDS             complement(25371..26282)
                     /locus_tag="ACHJ1G_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4763739.1"
                     /translation="MFHVDNNSGVANMPALAPVQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPGDVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     DQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPKNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(26282..26842)
                     /locus_tag="ACHJ1G_001876"
     CDS             complement(26282..26842)
                     /locus_tag="ACHJ1G_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4763740.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            complement(26833..27915)
                     /locus_tag="ACHJ1G_001877"
     CDS             complement(26833..27915)
                     /locus_tag="ACHJ1G_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4763741.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(27915..28352)
                     /locus_tag="ACHJ1G_001878"
     CDS             complement(27915..28352)
                     /locus_tag="ACHJ1G_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4763742.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(28345..28959)
                     /locus_tag="ACHJ1G_001879"
     CDS             complement(28345..28959)
                     /locus_tag="ACHJ1G_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4763743.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(28949..30073)
                     /locus_tag="ACHJ1G_001880"
     CDS             complement(28949..30073)
                     /locus_tag="ACHJ1G_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4763744.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(30057..31406)
                     /locus_tag="ACHJ1G_001881"
     CDS             complement(30057..31406)
                     /locus_tag="ACHJ1G_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4763745.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIIDPLFIPGGHSVEVISEQQG"
     gene            complement(31393..>31888)
                     /locus_tag="ACHJ1G_001882"
     CDS             complement(31393..>31888)
                     /locus_tag="ACHJ1G_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO4763746.1"
                     /translation="MGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAM
                     AAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWL
                     TEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGN
                     YGLW"
BASE COUNT         8005 a         8334 c         7978 g         7571 t
ORIGIN      
        1 caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt
       61 atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac
      121 tgtttatcct atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc
      181 taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac
      241 gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc
      301 acctaaattc gctttaccag ccggtagtag caaaaccgta cgttttgttg cgatggaacc
      361 agagcaaaaa gagaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt
      421 tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt
      481 tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt
      541 agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag
      601 caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa
      661 tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact ggatcaccaa
      721 agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa cgaaggaata
      781 gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc
      841 tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat
      901 atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg
      961 cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat
     1021 gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt
     1081 gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt
     1141 accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg
     1201 atgtttgctc aaatcaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc
     1261 atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg
     1321 gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga
     1381 taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac
     1441 caaaattccc gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta
     1501 tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt
     1561 tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc
     1621 gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg
     1681 ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta
     1741 taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga
     1801 tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca
     1861 agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc
     1921 gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc
     1981 agatgatgtc agtgacagta atgctgacat cagcgatctc tattatcgtt atgatttttt
     2041 acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg
     2101 cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc
     2161 gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct
     2221 ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct
     2281 caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc
     2341 attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac
     2401 cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca
     2461 ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca
     2521 atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa
     2581 taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg
     2641 taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc
     2701 gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga
     2761 gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt
     2821 cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg
     2881 ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat
     2941 ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag
     3001 tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt
     3061 gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc
     3121 aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca
     3181 gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg
     3241 aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg
     3301 aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc
     3361 ctttacgggc acttatagct ctacgctggc tgtcagtcgc tatggcgtta atttgggagc
     3421 cagtggtaca gacggtttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca
     3481 ggatgaagag agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca
     3541 gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga
     3601 ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg
     3661 gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt
     3721 taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt
     3781 ggggctgaac agcgccatgt tactggtagc tgaagatggc ggatttacac ttgaaattaa
     3841 cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag
     3901 tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac
     3961 ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg
     4021 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
     4081 atattgggct tgtttggttc aggcagctcc tgacgtgacc agtgaaatta cgtatgattt
     4141 ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag gaaataatgg
     4201 atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag ctaatggaaa
     4261 tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt ggccggtaaa
     4321 actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgccag gatgcaaggg
     4381 gggcaaaggg cttcagacga atcttaagga ttctaataga tcttcatgta cagaagatgg
     4441 tcaacattat tatatatacg atacaaagtt tcttacgctc taccttgagc agacagagat
     4501 gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt aaattacatt cgaacagccc
     4561 ggcccaggac tatttcgcaa atattaccct gaatacgctc gaccccaacc atattgacgt
     4621 gttcttcccg gagttcgccc acgccacgcc aagggtgcag ttagacttgc atccaacagg
     4681 aagcgttaac ggcagcaact acgcgcaaga tctgaccatg ttggacatgt gcctgtacga
     4741 tggttttaac ggtaatgcca tcagttatga aatcatgctc aaagatgaag ggcgaccagc
     4801 tgcagggcgc agagacggtt acttctctat ctatcgtcag ggagggacca ccaccgacga
     4861 gggagaacgc attgattacc gggtcaaaat gtacaacccg gaaaccggtg ggcaaattga
     4921 tgtgcgcaat aatgaaaata tggtctggaa cagcattaac ctgaaacgtg tgcgtccggt
     4981 ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg acacccttga ctctggcagt
     5041 cgataaattc agcgtgatgg ataaacaggc cgggtactac atgggcaaat tgtcagtaat
     5101 ctttacgcct tccttgccaa ccatcaatta accctgcaaa ccggacaaac gcttttttct
     5161 atataaacga cgaagccgtc tgataaagcg gcttcgtcat tacaacttaa gttaaacgtt
     5221 aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc ggtttgttct
     5281 gcaacacggt ccagataacc agcgcaccta acaggtcgaa cactgccaga actgcgaaca
     5341 gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac agcgtacttg
     5401 ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta cgaccgaaca
     5461 cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa ccaccgatac
     5521 acagcagcat aattgcgaca tacgggttgg tgaacagacc gatcataccc gggccaatca
     5581 tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc acaccaaacc
     5641 aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca gcaaacagca
     5701 tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact ttaaacatga
     5761 acagcgggat ccacgcgtta aaagtacccc aggccggttc tgccaggaaa cgcggcagcg
     5821 cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc ttcgccgtgc
     5881 tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca tcggtcagat
     5941 gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag ataaagctca
     6001 atgcaccgga gatgataaat gccatctgcc agctgtgcat tacgattgcc cataccacca
     6061 gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtaacct accgcgatgg
     6121 aacgctcttt cgccgggaac cattcggagc tggctttcag acccgccggg atcatcgctg
     6181 cttccgcggc accgactgca ccacgagcaa tcgccaggcc accccagcta ccagccagcg
     6241 cggttgcacc acagaacacg gcccacagca cagcaaacat tgcataaccg attttcgtac
     6301 ccagcacatc cagtacataa cctgctaccg gttgcatgac cgtataagca gcagaatagg
     6361 ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc agagttggcg
     6421 cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg
     6481 cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc gttttgttat
     6541 atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt acgaccgggt
     6601 aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc taattcggct
     6661 tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt gcattccgtc
     6721 gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa ggtgagagcc
     6781 atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc cagtactggc
     6841 gcggcctgca gagagattta ccactttgag agtaattttt ttaactacgt ttattgatct
     6901 aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc agcatcgcac
     6961 cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt tatgactgaa
     7021 gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc aaaagaccag
     7081 ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt
     7141 aaaaacctgt atgacatctg gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc
     7201 aacggtgtgg ccgagcgtct gtgtaccggt gatgcgtctg accgtgaaaa atttgacgcc
     7261 tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac gcacctcgaa
     7321 ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc
     7381 tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg catcatgcag
     7441 cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct ggagcatcac
     7501 gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg gcgtccggac
     7561 aaagccttca acatcgaaca ggcgaccttt aacgactaca tggcgaagct gggcgaagtt
     7621 tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa acgtctggat
     7681 cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt gatgtttgct
     7741 gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg
     7801 agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg tgccgaatat
     7861 gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa caacctgcgt
     7921 cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg tccgatggca
     7981 gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt
     8041 ctgtactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg taacttccag
     8101 ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa cgatcagaaa
     8161 gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtttgctgag ccgctttgtc
     8221 ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata cttccgccgc
     8281 attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac
     8341 ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta cttcgccatt
     8401 gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg ctggataacg
     8461 tcgcggtcgc tttagcggat ttggctgaag gcacagaagt cagtgtcgat aaccagactg
     8521 ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat atcgcaaaag
     8581 gggccaatgt catcaaatat ggcctgccga ttggttatgc attggcggat attgcggcgg
     8641 gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat cagtatcgct
     8701 atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc
     8761 gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgcca accgtgggct
     8821 gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc aacaacgccg
     8881 aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgctcacaa ctgggcgacg
     8941 atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag
     9001 tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg
     9061 gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat gatgagatcg
     9121 aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat aaacgcgagc
     9181 caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac ggtctttctg
     9241 gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct aacggcggta
     9301 ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg atggaccatt
     9361 gccgcgacga agcaacgttt gaaaaactgg tgaccatggt caacgacttc aaacagtact
     9421 ttattgccca tgaccagccg atctatgaaa acccatcgcc ggggaacaaa gcgggcggta
     9481 tcaccacgct ggaagataaa tcccttggct gtacccagaa agcgggttcc agcgtcgtgg
     9541 ttgacgtgct gcgttacggc gagcgtctga aaacgccggg gctgaacttg ttaagtgcgc
     9601 cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat atggtgctgt
     9661 tcagtactgg tcgtggcacg ccatacggcg gctttgtacc gacggtgaaa atcgctacca
     9721 acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt cagctgatcc
     9781 acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt gagtttgcca
     9841 acggtaaaca aacctgcaac gagcgtaacg acttccgcga actggcgatc ttcaaaagcg
     9901 gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca taatgtgaag
     9961 cgccgtttgc ttttccatac ctgataagga tttatcatga ccgcgtattg gctggcccag
    10021 ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg tgacgaacgt
    10081 cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt ttttcttctg
    10141 ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt gattacctta
    10201 cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg aattggcctc
    10261 gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt cagtacctgg
    10321 gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc
    10381 tgggtgattc acaacttctg ggcggggtcg ataggcggca caatgattga gggtagtttt
    10441 ctgcttatga atggcttgaa tatcattcgt ttctggcgga tgcagaaaag gggaattgat
    10501 ccgtttaaag tagagaaaac ccctcccgcc gtagacgaaa ggggttaaac aattaattac
    10561 gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca gtgaacagca
    10621 cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg atgataaagc
    10681 cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta caggccagcg
    10741 ggatcagcag cagagaaccc cccgccacgc cggatgcgcc acaggcacac agagaagcca
    10801 ccacgctcaa cagcagcgcc gtgggcagat cgaccgggat acccagcgta ttaaccgcag
    10861 ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg gcacccagcg
    10921 gaatagaaac ggaataggta tcgcgatcca gattcagctt ttcacacagt gccatattca
    10981 ccggaatgtt cgccgcagag ctgcgggtga agaaggcata cacgccgctt tcgcgcaggc
    11041 acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc aatgggttaa
    11101 ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc gcgtagcccc
    11161 acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagaccaaaa ataccaatcg
    11221 gtgcgaagcg aatgaccagt ttcaccataa aggtgacggc attcgacata tcgttgacca
    11281 ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc gaggccaatt gcccacacca
    11341 ggatcccgat gtagttacct ttcagcagcg cgtcgatggg gttggaaacc atgctcatta
    11401 ccagcccgcg catcacttct acaatgcctg acggtggcga aatatcaccc gcgctactgg
    11461 ataagtgcag ggtagaaggg aaggcgaagc tgaaaacgac tgcggccaga gcagcagaga
    11521 aggtacccag cagatagagg aacaaaatag ggcggatatt ggttttctgc ccgtgctggt
    11581 ggttagcaat agatgccatc accagcatca acaccaggat gggggcaacg gctttcagtg
    11641 cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt gagatccatg
    11701 ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg ccatgagcca
    11761 gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt cattgtgttg
    11821 tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa
    11881 ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt ttaaagagag
    11941 gtcatatttt gcaacttaat ggttacattt atttgacata aaaataaagc caacgtgcgg
    12001 cttccagact gatgacaaca tcaaaaaagg actcggtcag tcccctcgcc ccgtttgggg
    12061 agagggttag ggtgagggga acaggccagc atcggtgcga aaaattaccc tcatcccgac
    12121 cctctccctg gaagggagag ggagaaaacg gtgctcatgt taatgacagg gtacacggct
    12181 taggcagatt aaaaattatc caccccgctg cttatcatgc cgataattca cccaggcgtt
    12241 gataataaac gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat
    12301 atggtagaag tcgacaatca gcatcttgat accgataaac accagaatca ccgccaggcc
    12361 atatttgagc atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag
    12421 gccgaggatt gcaaacaggt ttgaggtcag cacaataaac ggatcagtgg tcacggcgaa
    12481 gatagccgga atgctatcca cggcaaaaat cacgtcgctc aattccacca gaatcagtac
    12541 cagcatcagc ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc
    12601 gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat
    12661 gcctgattca tcttcatggg cgagcgccat cttcacgccg gtaaacagca ggaaggcacc
    12721 gaagatatac aggatccagt cgaactgtga aatcagccag ctgcctgtga agatcatgat
    12781 ggtacgcaga acaatcgccc cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc
    12841 cggaacagag aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga
    12901 tttctcaatc agatagcccg tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc
    12961 ttcagtttgc accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca
    13021 gaccagcgac caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg
    13081 caacaacagg tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa
    13141 cggcgtgccg acagtattca tagaagttcc ttacacataa aaaaacggcc aacgtcggaa
    13201 gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct cacttacaac
    13261 aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat gtggtttttc
    13321 acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg taacaaagta
    13381 tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt tttactttgg
    13441 caattatagt tttacgctat ctgccggaaa aatcacccca gtctggcggc ggatctcggt
    13501 cagcagtttg gcggtgatgc gactgaccgc cagccccgga tgatccacca gatgctcatc
    13561 caccagggta gcgaacagct ctgcttcgta gagcatggta ttaatatgct gcggctgggt
    13621 gagatcctgc atttggctgc cacgcggcac gaagcacact ttctggcatt cagacagttt
    13681 ttcaatcacc agcgatcctg cttcgccctg aatctcgctc gccaggacag aatcactgac
    13741 ttttgagtgc tgcaaggtga cgctgaaatc accgtaatcc atcaccacca cgccgtgggc
    13801 atctacaccg cttgccagca aactggcggt tgcctgcacg cttttcggct caccaaataa
    13861 cgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt tagagaatga
    13921 cggattaaag gtgttgggat tttcaccgtc caggtaacgt tgatagcgcg aggaatactg
    13981 gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct ggcgcagcaa
    14041 atgaaagttc ggcaggcagg cggttttaaa tgcctcaaac agcaccacct gattttcccg
    14101 cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt tctcgcaaat
    14161 cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct
    14221 ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg aggtaaacag
    14281 atgctcgaca gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac
    14341 ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc gagtgatcca
    14401 gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa agggattctg
    14461 tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt attcttttgc
    14521 cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat atttcggctg
    14581 gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct cccgcagttg
    14641 agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag cgtcgatgcc
    14701 gtactgtttt gccagccaga tggcgcgttg attatgaaat tcttgcgaga taatggtgat
    14761 atggttttca ccaaaaacct ttttggctcg cactaccgaa tccagcgttg agaatccggc
    14821 atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaagcctg catacctgag
    14881 gcttcatcgt aatttttacg tccgttatcg ccacttacca gtagccattt cacttttccg
    14941 gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctg
    15001 gcccccagca ataagcccac gttacgcgcc ggaactgcgt tgacatcgct ccaggtcagc
    15061 tgcttgctgg cgttgaccat aacgcgatcg gcaataaaaa tggtcgctcc tgctataagc
    15121 aacaacaaac agccaatctt aagagtccgg cgagaaaaac aaatacggag aagaaggcgg
    15181 ggctgcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag
    15241 aaaagataac gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgcgttag
    15301 ccacagccgg gtgtcgaact caagctggtg gtactgcggc tccatatggc agcacaactg
    15361 gtaaaacgct ttgttatgct ctttctcctt caggtgcgcc agttcgtgca ccacgatcat
    15421 acgtaaaaac ggttccggcg cgttgcgaaa cacagtagcg acgcggatct ctgcttttgc
    15481 tttcagcttg ccgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag
    15541 cacatggatc ttattgtcat acatcacttt attgatcggc ggggcattgc gcagaaactg
    15601 attttttaga tccggagtat attgccagag ggctttatcg gtggcgtagt cgtgtgttcc
    15661 cggatagcgt tttgccagca catcgcccag acgctgttcg ttaatcagcg ttcgcacctg
    15721 ggaaagtagc tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga
    15781 aaaagagaaa aagggtatac tcacgcaccc ttttcagggg caacgtcgaa attttatcat
    15841 tcaggagggc cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt
    15901 ccggcgacgg atgacgttaa cccgctacag gcgtgggaag cggcggatga atatttgctg
    15961 caacagttgg acgacacaga aatccgcggc ccggtgttga tcctgaatga tgcctttggt
    16021 gcgttaagct gcgcgctggc ggaacataag ccgtacagca ttagcgactc atacatcagt
    16081 gaactggcga cgcgcgagaa tttacgcctc aacgggattg atgagtcgag cgtgaagttt
    16141 ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa
    16201 acactggcat tgctggaaca acaactgcgt gcgctgcgca aagtggtcac gtcggataca
    16261 cgtattattg ccggtgccaa agcccgtgac attcacactt ccacgctgga actgttcgaa
    16321 aaagtgctcg gcccgaccac caccacgctg gcatggaaaa aagcgcgcct gattaattgc
    16381 acgttcaatg aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt
    16441 actgactgga ctatccataa ccatgcgaat gtcttctccc gcaccgggct tgatatcggc
    16501 gcgcgcttct ttatgcaaca tctgccagag aatctcgaag gggagattgt cgatctcggt
    16561 tgcggtaatg gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg
    16621 tttgtcgatg aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg
    16681 ccagaggcgt tggatcgcag cgagtttatg atcaacaacg cgctctccgg cgtggagcct
    16741 ttccgcttta atgctgtgct ctgcaacccg ccgtttcacc aacaacatgc gctgaccgat
    16801 aacgtcgcct gggagatgtt ccaccatgcc cgccgctgcc tgaaaatcaa cggcgagctg
    16861 tatattgttg ccaaccgtca cctggattac ttccataaac tgaagaagat tttcggcaac
    16921 tgcaccacca tcgccacgaa taataaattt gtggtgctga aagcagtgaa gctggggcgt
    16981 cgtcggtaag attgggagtt acctgccgga tgcggcgtta acgccttatc cggcctacgt
    17041 tcagcacgtt actcccaggc cggataagac gcggcaagcg tcgcatccgg caatgtctgc
    17101 ccagttctgg aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc tctacattct
    17161 cttgtttagc gttgttcaac gtttatgcta catacgtgaa gttatgacgc ataaagaata
    17221 cgatttcttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat tgccgacatc
    17281 ttgcaagcag gagaaaagct aactgctgtg gcaccttttc tggcgggtat tcagaacgag
    17341 gaacaataca cccaggcgct ggaactggta gatcatctgc tgctcaacga tcctgaaaac
    17401 cccttgctgg atctggtgtg tgcaaaaata accgcgtggg aagaatcagc gcccgaattt
    17461 gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat tcgtaccctt
    17521 atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa atctatggtg
    17581 tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa attggcaacg
    17641 cgattcggca tttctcccgc cttgtttatt gattaacgcg acgtgccgga tgcgttgcat
    17701 ccggcgcttc aaataacgat ttaaatctcc agcgccagcc gtgttccctg ggcaattgcc
    17761 cgtcgtgcgt ccagctccat agccacatcg cagccgccaa ttaaatgcac agttttcccg
    17821 ctatcaatca gcggttgcgc cagcgcgcgg tttggctctt gccctgcgca gatcaccaca
    17881 ttgtccactg ctaatacctg cgtttcgccg ttgatcacca catgcagccc gtcatcgtca
    17941 atcttctgat aacttacgcc tgggatcatt ttcacacccc gcgagagcag ggtggtgcga
    18001 tggatccagc cggtggtttt gcctaacccc tgtcccggtt tgctggcttt gcgctggagc
    18061 atcacaattt gccgtgggct acgcgggatc tgcattccct gcgggcttaa gccaccagcc
    18121 tgttgtaggc tgctgtcgat cccccactca ttacagaacc tggcgatatt ctggctggtg
    18181 gattcgcccg gctgacttaa atacatcgcc gtatcaaaac caatcccgcc acaaccgatg
    18241 atggcaacct tgttgcctac cggcgctttg tcgcgcagta catcgagata actcaatacc
    18301 ttcggatgat cgatcccgtc gataggcgga acgcgcggca cgatcccact ggcgaggatc
    18361 gtttcatcga acgcctgtaa ctgatccgcc gtcacggtgt gattgagttt cagcgtcacg
    18421 cccgtcactt cgatcatccg gtggtaatag cgcagcgttt cgtaaaactc ctctttgccg
    18481 gggatctgtt tggcgatatt aaactgcccg ccgatctcgc tgtgggcatc aaacaatgtt
    18541 acatgatgcc cacgcgccgc cgcgttaatg gcaaacgcca gcccagcagg tcccgcaccg
    18601 accaccgcca gatttttttt ctgcacggca ggaaggattg gcattttggt ttcgtggcag
    18661 gcgcgaggat tcaccaggca cgaggtgact ttgccaacga agatctgatc gaggcaggcc
    18721 tgattgcagc caatgcaggt gttgatctca tcggctcgcc ccgattgcgc ttttgacagc
    18781 agttccgcat cagcaagaaa cggtcgtgcc atcgatacca tatcggcatc gccgcgcgag
    18841 agaatgtcgt cggcaacctg cggatcgtta atccggttgg tggtcaccag cggcagcgaa
    18901 acgtggcctt tcagtttgcg cgtgacccaa gtaaatgcgc cgcgcggcac gggcgtggca
    18961 atggtcggaa tacgtgcttc atgccagcca atgccggtgt tgataatggt cgcgcccgcc
    19021 gcttcaatgg cctgcgccag ctctaccgtt tcggcaaaag tcccgccgtc ttcgaccagg
    19081 tcgagcatcg acagtcggta gataataatg aagtcgttgc cgacgcgttc gcgcaccgca
    19141 cgcactactt ctacggcaaa tcgcatccgg ttgcggtaat cgccgcccca ctggtcacta
    19201 cgctgattgg tgcgcagcgt cagaaattcg ttgatcaaat acccttcgga ccccattacc
    19261 tctacaccgt cgtatcctgc ctcccgtgcc agttgcgcgc agtgggcgaa atcgtcgatc
    19321 agttgcagga tctcttcatg ggtgagttcg tgaggaacga aacggttgat gggggcctgc
    19381 aatgcggagg aagcgaccag atgcggttgg tagctgtagc gcccggtatg caaaatttgc
    19441 agggctatta tagcgcggcg atttttttga taccgatttg catttcattt gataccgctg
    19501 aaaaacgccg atacgcaact tatttgatac taaatcggcg cttttctttt gagttttaga
    19561 atgagttttt aagcgctcat tcactctctt tcaaaccagt tttaactgtt tgtggttttg
    19621 ggtaagggtg gactttgaat agctttgtgt tgaggcgttt tctggccctc ttgttcagaa
    19681 accggatgta acgaaactgt cggaacttat gcacactggc tctgtcgata ttagccctca
    19741 gatgttcacc tcgttgtccg cctcgtttaa tggcactcct gcaaatctcg tgataccact
    19801 cgccatcaag ttcatagaat gttgattcat ggctgcctac ataatcaaaa ttgctggcct
    19861 gatacacgac accaagacag ccacaacgct catctgcaaa cgactgaacc cactgtacct
    19921 gcggataaag ctgcctgatt agcttgagcg cgtaactgat tgcccttgat tcggagtttc
    19981 tcggcataca gtcatgcatc catagccggt taagctccat atattctcgg ttctgcgtac
    20041 ccgttacgac gcgtgcacca ctgtttgggt taagggcgta accccattgc attacgccaa
    20101 caagttcccg ttctgaaaat atacccagat gaaggtagga attatttacg aaacggtggc
    20161 tgtaatgttt attgacgata accagtctgg ccagccagca acttattgtc tcaacccgca
    20221 actcacggga gccataacca acgatattgt cgttatagcg aataagttca ggcgtgctga
    20281 tgatacggga tgtgacttgc tttctgttcc ccacgacagg atttccttgt gtatgtgggg
    20341 tgctctgtgg cgctcggaga tgtgatttga ttgagggttt tacagcgcgg acattttact
    20401 tccagataac tgaaactggc ccgtgccagt agcttattgc agtgtcggca ccgtatgttt
    20461 cgatacatgg ccgcataacc tcctgctttg ttgttgatgc ctttctcatg caatcgatcg
    20521 acaaaaacga tcgattcgat ttgtttaatt gatacaacaa atagatttaa aagtcatcaa
    20581 caatgattca gggcggtgat tatttactta aagaaacgaa gagttaggtg gtgaaaacga
    20641 tcataaaatg atcattttat tgacttttta tacagtgaag gtaggatcgc gcgtatatgt
    20701 agtatgtttc aaatcaacag gatactacat aaaagtgaaa cccagcatag ctggaactat
    20761 gctgggtcaa tctgtaaaaa cttgcatcaa tgctttactt cgagctcgtg gcgtatccta
    20821 tgatattccc cggtctcctg taaagcagag atgcataaaa aggagatctt tatcatggat
    20881 aaagagcctg tttgccctgc ctgtaagact cccttacagc tcatctatcg ccgttctcga
    20941 tctcaaaatg gcaagacata caaaccaaaa aatgcttcct gtttcaggat gctgatatgt
    21001 cctgcctgtt ctccccggag ggaaaataca caaaacgtgc attaacaaat tcacccggca
    21061 gggaaacctg ccggtcggta ttgttacatc atagtaccgt cagttccggg ctgaaccggc
    21121 tctggttgct ctacgggttg aggctcattt tccgtgctgt tttcagccgg agaacgacca
    21181 accattcggg ccagcacttc atcaacatat gcgtcgattt gtgcctcaaa atccttgcga
    21241 acctgcgcct tcagcacgcg gttcacttct tcagaataaa gtgcctgttt gacaagctct
    21301 tcagtaaccg tgcctttaat ctctggcatg aatattctcc tttccttttc tggttcacaa
    21361 aaaattaaat ctgaccggag tctttcggct gcggcattac agcaccgctt tttatctttt
    21421 cctcggcagc tgcatcaatt ttatttcgaa aataattgcg aataatttta taaccaccgc
    21481 tgacaagcaa cagacaacaa accaccgtgc agaaatacag caggcagacg tttatatatg
    21541 tcatataagc ctcactctta ttaactgatg ttacagccgg ataaattccc ggccgtcacg
    21601 attatgccgc aggggtatta acctcaagga caacgctctc aaaagacacg ccataagttg
    21661 cccctttggt gacatctgtc agtgtcagcg tatctttggc aggggaaagt gcatctttca
    21721 ccatctggaa tgcctggccc ggagtgccat ccagcacagg cgttacctgg gcacttcccg
    21781 ccgtaaactt caggacaagt gtatgccact ggttattgta ggctccaaat gatcccagtt
    21841 tcgtattccc ccctgtttta tggtgcatca gatttaactg gccgcctgtt gtctggagat
    21901 aatacgccat caggaacggt gtgcctgtac cactcatggt tacaccttcc gggaccgtat
    21961 cggtgtggag gtaaataccc agagcaaact ggccttcttt tacttcacct gacagacgga
    22021 atttacaggt caactgacca ccatgcttca gaagttcacc ggctgcatca gttccatggg
    22081 taagcgtcca ggtttttccg ctttgtttgg atacggtcaa cgctttacct tcgtccgtca
    22141 gctggattgt accatctgta attttccagc cctgagccgg ggcttctcct gctgatgctt
    22201 taagtgaaag cagcgtacgg agtgacgggg atgtcggatg gggggactca ctgcctcctg
    22261 gctgactacc tgtcggcgct tccggtttcc cacccggcgc gggctgaggc agggcttcac
    22321 catcaggggc cacaccggca ataaatgcag aggtcagacc tgcatattta agaattgctg
    22381 atgcaaaacg atctgagacc agaaaacggc gcgcccagga gctaaaatgg gttgccctgg
    22441 atgctgttgt ccagttgcca tctccccgtg attccgcacc ataatacccg gcagaagcga
    22501 tatccgggtc gtgctccggc aggttggtcg cggttttatt accgaactca tcgtgcatca
    22561 gtggaacaaa gtgaatgttc tcggaagccc ggtttttata tccgctataa atggtttcat
    22621 atgccgccgg gttcttctct ttccagtcat aggttgtgtc accacataac cacggcacac
    22681 tgtctgatga accaccaata cactgaccgg agaaagcacc aagatccgca cggaattttt
    22741 tcaccatcgc agtaaactgg gcaccatgct gggccggggt cgctttaagg tctccttccc
    22801 cctgaatcca tacgacagat aacagcacat tctttggatt cgcctcaagt gccgctttag
    22861 ttcgactgac aagatcctga tacagtgggg tatccactcc ccagcgtagt gacgcctcgg
    22921 ctgcaccact tgccggatcg aatgagccaa ctgcgccggt tgtaaaagca gagccgccac
    22981 gggcacaggg aaccagtaaa atcccggcat cagcaggaat caccggtaat acttttttcg
    23041 caatatgaag tccctgcccc acacaaccat actgcccttt tttcaggtct gcttttggat
    23101 ggttcagcag actcatattc tgaacatcat gaaggcagtg atctgctgga atgatttcat
    23161 tgtatttgca gggaatacct ccgggggttt gtgtattcct gcgtgccagc tgcataatac
    23221 gtggatcagg acgatcataa gaatccggaa ggggcaaccc ttcaccataa gatgaagcat
    23281 tggactggcc ggctataaca atcacgtaat accaggaagg ggagctggaa gccgtttcat
    23341 ttcctgccgg acgggtatgt gtttctgcat tatcccagtc tgacggttca gcagtaccgg
    23401 caacatcagc acttaacgga atgacagcac caatacccgc tccggtaatt tccacgttcg
    23461 gatcaccgga tactggcctt acccacaggc aatcctgtgt attaaaactg atgagacagt
    23521 cagcaaatgt cattcctcct ttcgtttcag caggaggaaa gggagcctca agaaatgccg
    23581 ctgtaccacc ggaaacttta acggtaaagt ttccgggcat tgatgatagt tgaacccatt
    23641 tggacgtcat aatattcatc ctgttcctga ataaacagaa ttatcatatc cacagaccga
    23701 taaacgccat tttgcgttaa ttcagtaaag gcccaacaaa gggagcgtct tatttttccg
    23761 aaacgctcgc aggataataa cgataaaggg ttgaaaggcc gatatcaaaa ataagtgcca
    23821 cctgtcttcg actgacaccc ccggccagta aacgcccgac ctgctcgtat tgttcctgcg
    23881 tgagacgacg gggtctgccg ccggttcgtc ccctttcacg ggcagctgca agaccagccc
    23941 gtgtccgttc gacaattaat tcacgctcca tttctgccag cgcacccatt atgtgaaaaa
    24001 agaagcgccc catgggggtt gacgtatcaa tgttgtcagt aagactacga aaatttatcc
    24061 cctgttgctg taattcatct gtcagcgcaa caaggtggcg catactgcgc cccagcctgt
    24121 ccagtttcca gaccatcagt gtgtcaccgg gttttagttt cttcagtgcc cgcttcagtc
    24181 ccggccgctg tgtcgtttta ccactcattt tatcttcaaa aatctgctca catcctgcac
    24241 attccagcgc atttctttgt aaacctgtgt tctggtcatt tgttgatacg cgtatatagc
    24301 caatcagcat gtatttacct tccttaaaaa gaggtgaaaa tgccttttaa aacagatata
    24361 cggcatccct gaaacctcgg tttacgggat actgtaaaca gggccgcgaa tgccctgcca
    24421 tcgaacggag cggctgttgc agcgaataaa ctcgccactc caagaaatat taatggtgtc
    24481 ccatttgacg gaacgcagga tataaatatt acatcgggga tgacgcagtc tgtggctgac
    24541 tcacgttatg ttcaaagcat tcgaagaggt tcaaggtcaa cgattggaat gcaatataat
    24601 atttttgagg ttcctgacgg atgcgtactt acagggcttg atgtcgctgg tgatggtaat
    24661 gcaacggtaa ccgcatatta tcgacctgtt cagtttctga tagatggttc gtggaaaaca
    24721 gcttccagcg cataaaagtg cagtagttca aaaaacagat tatctgaata aatatatgat
    24781 gattttgtct tttcaacaaa aacaaggtga aataatggag cttattaacg taaaacgata
    24841 ttacccggaa cacaaaccct atggtgagga tgttcagtat ttccagagtg aggacggaag
    24901 ggatttttat gaatctatcc cgcttttcac aaagaaatac aaactctgta tttcgcctgt
    24961 aacgggtatt atctgctctg ttgctgaaga tgtctctgct ttatatccgg caggttttac
    25021 tgttgttgaa gtggacgaac tgccggaagg tgtgaatatt gacggtaact ggcagttttc
    25081 tgatggcctg atatcaaagg tgccggtaaa ctggaagacg gttgctgaaa agcgtcgctc
    25141 atcgctgctc caggaagcca acgaaaccgt ggatgactgg aaaacagaat taaaactgga
    25201 catgattagc gatgaaaata aattacagct gacgcgctgg atggcgtata tccgacagct
    25261 taaagaaatg cactttaatg atattgccag tgaggggcat taccaggcga ttccctggcc
    25321 ggaaaaaccg gaataattca gagggtgcat tcagcaccct tttctgtggt ttacactgaa
    25381 cttaccgttc tccaggcatt attaatataa atctgaaccg ggcgataata gaccccaccg
    25441 atattgtccg cagaccggga gccggtttcc tgcacgttaa taccggaaag catacaaccg
    25501 gaaggtgcgc tgtaattcca tgatatttca ttgcctccgg gtgaatgata gctctgtgct
    25561 ccaagctgaa cattctgtac ataccggcca tctgcggttg actgcgtcat tcctgacgtg
    25621 atgttgatat cctgcgttcc gtcaaaagga acgccattaa tattttttgg ggtggcgagt
    25681 ctgtttgctg ccacggcggt tccacccgat ggcagggcat cagcagcctt atttaccgtt
    25741 tcccgtaaac cgacgttatc gataaaagcg cctttatccg gaatgtctgc gccattctgg
    25801 tccctgcgca ggtaacggtt atcaccttcg ctcttgctgt aaacatccag attattacgg
    25861 gcggttccct tattctccag gtcggcgaga ttctgatcct tcgccagggc attaacgtca
    25921 ccgggaccaa gactgttttt tgtggcaagc gtccccagac caagataacc acgggccaga
    25981 cgctgggcct ccgcaccggc atcagcaatt tctttcaggt tatttttccg ggaaaaagca
    26041 ttcgcagcga taatggcttt aatagacagc gtaagctggt ttaactgcgc cttatccggc
    26101 tgaatactgg cttcagccag aatattcagc agttcggcct ggagaatatt cagccagtcc
    26161 tgaccaatcc agctgatacc tttttgtccg tcaccttcgg taaaccaggt ggtggtatta
    26221 ctctgcaccg gtgccagcgc aggcatattc gccacgccgg aattattatc aacatggaac
    26281 attaaatatt ctcctcttcg tgactgtttg catatacgta aataaacgtc tgccatgcag
    26341 gcttataacg gttcagaata cattccagcg caccgccttc ataaatacgc agcggcgtga
    26401 gaatatcatc cagcacattc atattccggt agccggtttc actgttaatc gtgacaatac
    26461 tgacccactg tgaatccggt gatggctgaa tatctatttc ataaccaaac tctgccgcaa
    26521 accggatata aaattcacgg ttaagagagg gtttcatccg gtatttattc ccggcataac
    26581 gctgacgctc ctgaatggtt gcgccggtca tatcacattc cggtaacccc agataacgct
    26641 cccagtcttc cagcagcaga cgggatgaat caggaaaacg ttctgccagc atctggttac
    26701 cggtccagga aacacgctcc gtggaatgac tgaggcccag gcacaaagcc gcaagaacgg
    26761 atgacggccg cttatcccat gccagcccgt caggcagcaa ctgcaacagg gcgcgctgat
    26821 gcggggtcag ggttacagcc atgtgatttc ccccacggtc agcaactctg tatctccggc
    26881 catcacggac ttcagcgggc tgcgaacttc aaaatcctcc agtcctttca cccctgcaac
    26941 agcccgccag aatgatgaag gcaatacaag tccacccggc cgcgattcgt tataaagcag
    27001 gtctgttaat gcctgtttta cggcagcctg attttccggc gttttgggta caatcctgat
    27061 ctcaaacgcc accggcttat tggtcagctt aaacacgctg attgtcggcc caagtggctg
    27121 accaacgggc tggcccgtcg ccggatcatc atgactgcgg atataatccg ccacccgctt
    27181 cacatcacct tctccgggga aaatgtcagg gttattatcc tgaacaaaag tcacaccaac
    27241 actacccgcc tcaggccatt caggcttaca ccatgcccgc gtcacgcccg gtacttcccg
    27301 tgcccagcgt tcaaaatcat actgtgttcc ccctgatggc gggttctgta cccggaatac
    27361 cagcctggac agcaactccg gcaccgtttc cacatccgca ccaccggtga gtcccgtgcc
    27421 ggtgaccgtg gctgtctggt tgatgcccgc ctgtggcgtg ataaaggaca ggcgggttcc
    27481 ggtcggggta tttccagcgc gccccgcact ttccgcctcc acattaacat tcagtgttcc
    27541 ggcttttccg gtcgcagagc cggtgatgcg gtacacaacc ccatcgcttc gctgaaggag
    27601 tacgccttcc gtgatccctg catccgtggt cagcatcagt tgtaccggtc cgtcagcgcg
    27661 ggaagccggt ttacggatga caccccagaa tgcgcagtgt ttcaggagtt cggcttcatc
    27721 agcttctgtc ggaattatct gccgggagat ccaggccaga tgttcatgct cctgtgctga
    27781 cagacccgcc agtgcatacg caatggcatt cagggtggtt tcattcacgc ccggctgcga
    27841 accgggcagg cgctggctga tatcctgttg tgtctgtgtg atcagttgcg ccagtggtaa
    27901 gggctgataa ggcatcattc ccccttcata tcagcataaa aaatcatcgg atgtctgctt
    27961 ccgtccggca gggtgatttt cacggaaagc gaaaggcgag catgccccac acgcatggca
    28021 tgacatacca cggatgtggc agcaccgctt ttatgcagcc acgccagcgc ttcatcagca
    28081 taggcctgtg cacggctgac cacggaggac agcgtctttt cacggcttaa cagccagaga
    28141 cgggagccaa cggggcgctc ccggaaactg tctccccacc atccccgacg gtcacgggtg
    28201 ccgtcaggga tttcatcgga atccagcgcc cgcctgtccg taaacagtga aataatgacc
    28261 gcagtcagca ggctgttatc cgtcagaaga tctgcgccgt tcagttgcag ggaaccgcaa
    28321 ccctgatccc agacgacagc aatatcagcc attctctggt cctcccgttt tgctgccttc
    28381 accgttatcg tgatgcacat gacctgaata ggtgatcccg gcaataaccg cttcggacat
    28441 cgtgaacgtc ccctgtgatc ttccggtgcc gtcaatggac acattgcctt tgacatgcag
    28501 gttcttatcc actgtgacat taccggtaaa gtgagcctcc ggtgtatcca cctgaacacc
    28561 ttcatcggcg taaatctcca gcgtcttaca ggtcacaatg atccggccgt tctttgtcag
    28621 acgtatgcgg tgcccctcat ggtgatacac gccggtatct ccggcggtaa gtccggtcgg
    28681 gcgactgcgg cggtcttcca ccacaagcac cacggcctga tcccgttgtc cgccagcaca
    28741 ggccagaaaa acttccgcac caggcagggg gacgctgatt tgcccgtact gctgggggcg
    28801 ttcaacgtca tcaaaggttt caccatccat accggtgatt tgcacattct ggattttcag
    28861 cgtgtccgtt gtgccggtca gtacaccacg cccgaacagc agacgaacac cccgcatcac
    28921 gggggccaga aggcggctga tgacttcgtc attcatcatt gaatttaatc ccctgttttt
    28981 tcatctgtgc ccgcacgaag gcgtccacgt cacccgcatt tccgcttccg ctgtttcccg
    29041 gctctgccgg aacaataaag ccgtcacggg gtgccagaat cagacgggtg gtttcgccgt
    29101 gatttgcatc cagcgaaaac tccacctgac agacaagaag atcacgctct tccactccgg
    29161 tacggggagc cgtgacagcc gtcagcaggt tgatatccca caactggccg ttctcccgaa
    29221 accagtgccg cacggtggct gtcaggcgca cagagcgggc aaccgcccgg cgcatttccc
    29281 ggacagccct ctggcgtgca ccgtctgcat caacagcatg atccgccagc acaattttcg
    29341 gtctgtaacg gtggatggca ctgtctgtca ccgtaccaac tggtgcggcc agcgttccgg
    29401 ctgtcagtgc atccccggca tgaccaccac cacgcccgtg cccccgtaca cggtattcac
    29461 tgtggcgcag acggtgatcc acgttgtgat ccaaatccag cagattttcg cccagcgtca
    29521 gcgtgtcacc acgctgactg ccggcctgag tgaaaaccag ttcaccggcg gcattactgg
    29581 ttaccaggac gcctctgtgt ctcgcggccc gtgtcagcgc atctgccacg gtttctgaat
    29641 tttccagtgt gaaggtggaa aagggccggg ctgccgttgc atcatttacc tgccagcgca
    29701 ccgtgacccc gaacggagca cacaaatctg aagcaatgtg ctccagcgtg cggttcctcc
    29761 actggctgcc cggatgaatg gctgaacagt ccaccaggtc accggtttta tcccgtccgc
    29821 tgagcgtgat ctgaaaacgc gtggcgctga tgcgctgccg gacctgatcc agccagccag
    29881 tgatcacagg ctgaccgtca atgcgcaatt ccagagactg accggcacgg acggatgacg
    29941 gaaggcgtac accgggcatc attaccccca gttcaaagga gcccgccaga tgttcaagag
    30001 agcgacgaac actcaccgtc agccagccgg aaaaaatctc accgcccaga tacagttcaa
    30061 ccctgctgct cactaatcac ctccactgaa tggcctccgg gaatgaacag cggatcaata
    30121 ataccgttac ggcgcacaaa acgttgccag ccggtactgt tcccggtggc acggtacagg
    30181 gtcaccagtg caggctcggt ggtacgtaca gtcactactg ttgcaccggg tagctgaact
    30241 ccccgtgtat tcagatcctc tgtcagtgcc agtctggcat cacgaagcgt cagcgccgtg
    30301 gcggtatacc cctgcccgga cagtgtcatc acctgccgtt cgagcgcttc gcttaagtca
    30361 cggttaatgc gctgaagatc aactgaactt tccagccaga caggccaggt acggtgggct
    30421 gcgtcactgt ccggcagggt ttcctgactg agaacctgat ccagcagttt tcccacggtc
    30481 tgtgcctggg ccaccacggc agcactctgc attaccgccc gtatcagacg aatattcttt
    30541 tgtgctgccg gtgtcagacc gatgagagtc tgcggattat cctgctcatc cagcacgtaa
    30601 cgcagggaag ataatgtcct gtacagttgt ggcattccct gagagacggc ggatgtcgtc
    30661 cggccgatac tgccaccggg ccgggtattc aggcctcctg aagacagtga cggcagcgat
    30721 ggcagggtga tcagggcact gaaaagcccc gcaaaagatg aggccatgcg ggaaggtgct
    30781 gtcagtaatg ccgtggcatt ccctttcagc gccgtgaacg tggcagtaaa ggcactgata
    30841 tcctgtacaa tccccatgcc ggaaacggca ttttccaggg cggacacctt atcgctgatg
    30901 gtttcggtca ttgcctggac atcgtgcatt ccgtcagaaa tcaccgtcca gcccgcttcc
    30961 agggtttcaa aaaccttccc gagcgcggtg gtactcttct gctccagaat ggcggccgtg
    31021 tcctgtgtga cagcaggtgc ggtatcatca cttaccggcg tgacattaat ggtgaactca
    31081 accacgccct gttcagcagc gttataacga ctttcaaagc tgtttatcag cacatttaac
    31141 gtgccgtaat ccgggtgaag cagctccccg gcaccgggag cacgaagggc atcacgcaga
    31201 cgatttcgct gtgtctgaac atcatccccc atcacaagta cactgaacgt aaattcagcc
    31261 agtttaggtc ccagatcgtc tgcaccaccg gtttcccgta acggatattc ccgacggaca
    31321 atgtttcgcc cgccacgttc gcgctgctca cgccagacga ggaacggcac accacggaac
    31381 gcaccacgac cgtcaccaga ggccataatt ccctccgttc cagacattca catccagtcc
    31441 gtgaccggac gtgtcatcaa tatcaatact gcgagcctgc cacccttccg gggcaaccag
    31501 ctccacacgg gcagaggctt tttgtaccgc ctgtccactg ctttcatcat cactgccccg
    31561 taatttctgc caggcttcag tcagccatcc ccccagatag ttaccgagat agctgcccac
    31621 cgtagaacca atggcaatcc ccacaggtcc ggctgccgcc cccagcgcac caccggcaat
    31681 actgcctgca agagagccga ccgcttctcc cttgtctgtg gcactggcgc tgccatccag
    31741 cagaaccggg gctgccatcg cgccagccca cagggcacct ccacccagcc ggccggccag
    31801 tccaccggca ctgcggaaaa agccggagaa acgccccagc cccatgcggc taccgacaga
    31861 cgaaagtaac ccaccggcgc gccccatc
//
LOCUS       DAZFDP010000038        31106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFDP010000038 DAZFDP010000000
VERSION     DAZFDP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHJ1G_001883"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHJ1G_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4763747.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHJ1G_001884"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHJ1G_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4763748.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHJ1G_001885"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHJ1G_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4763749.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHJ1G_001886"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHJ1G_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4763750.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHJ1G_001887"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHJ1G_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4763751.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESMDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHJ1G_001888"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHJ1G_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4763752.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHJ1G_001889"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHJ1G_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4763753.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHJ1G_001890"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHJ1G_001890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4763754.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHJ1G_001891"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHJ1G_001891"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4763755.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHJ1G_001892"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHJ1G_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO4763756.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHJ1G_001893"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHJ1G_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4763757.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHJ1G_001894"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHJ1G_001894"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4763758.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHJ1G_001895"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHJ1G_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4763759.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHJ1G_001896"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHJ1G_001896"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4763760.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHJ1G_001897"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHJ1G_001897"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4763761.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHJ1G_001898"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHJ1G_001898"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4763762.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHJ1G_001899"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHJ1G_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4763763.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHJ1G_001900"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHJ1G_001900"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4763764.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHJ1G_001901"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHJ1G_001901"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4763765.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHJ1G_001902"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHJ1G_001902"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4763766.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHJ1G_001903"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHJ1G_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4763767.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHJ1G_001904"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHJ1G_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4763768.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHJ1G_001905"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHJ1G_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4763769.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHJ1G_001906"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHJ1G_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4763770.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHJ1G_001907"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHJ1G_001907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4763771.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHJ1G_001908"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHJ1G_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4763772.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHJ1G_001909"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHJ1G_001909"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4763773.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHJ1G_001910"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHJ1G_001910"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4763774.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7338 a         8068 c         8505 g         7195 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcatggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attcagggtt
    31021 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgcgcta aagcgcgtcg
    31081 caactgctct ttaacaattt atcaga
//
LOCUS       DAZFDP010000039        30859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFDP010000039 DAZFDP010000000
VERSION     DAZFDP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(162..1752)
                     /gene="lsrK"
                     /locus_tag="ACHJ1G_001911"
                     /pseudo
     CDS             complement(162..1752)
                     /gene="lsrK"
                     /locus_tag="ACHJ1G_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1831..2784)
                     /gene="lsrR"
                     /locus_tag="ACHJ1G_001912"
     CDS             complement(1831..2784)
                     /gene="lsrR"
                     /locus_tag="ACHJ1G_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO4763775.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3033..4568
                     /gene="lsrA"
                     /locus_tag="ACHJ1G_001913"
     CDS             3033..4568
                     /gene="lsrA"
                     /locus_tag="ACHJ1G_001913"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO4763776.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4562..5590
                     /gene="lsrC"
                     /locus_tag="ACHJ1G_001914"
     CDS             4562..5590
                     /gene="lsrC"
                     /locus_tag="ACHJ1G_001914"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO4763777.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5590..6582
                     /gene="lsrD"
                     /locus_tag="ACHJ1G_001915"
     CDS             5590..6582
                     /gene="lsrD"
                     /locus_tag="ACHJ1G_001915"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO4763778.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6594..7616
                     /gene="lsrB"
                     /locus_tag="ACHJ1G_001916"
     CDS             6594..7616
                     /gene="lsrB"
                     /locus_tag="ACHJ1G_001916"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO4763779.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7643..8479
                     /gene="lsrF"
                     /locus_tag="ACHJ1G_001917"
                     /pseudo
     CDS             7643..8479
                     /gene="lsrF"
                     /locus_tag="ACHJ1G_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8478..8972
                     /locus_tag="ACHJ1G_001918"
                     /pseudo
     CDS             8478..8972
                     /locus_tag="ACHJ1G_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; internal stop; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
     gene            complement(8976..9890)
                     /gene="yneE"
                     /locus_tag="ACHJ1G_001919"
     CDS             complement(8976..9890)
                     /gene="yneE"
                     /locus_tag="ACHJ1G_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO4763780.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10097..11548)
                     /gene="uxaB"
                     /locus_tag="ACHJ1G_001920"
     CDS             complement(10097..11548)
                     /gene="uxaB"
                     /locus_tag="ACHJ1G_001920"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4763781.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11775..13193)
                     /locus_tag="ACHJ1G_001921"
     CDS             complement(11775..13193)
                     /locus_tag="ACHJ1G_001921"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4763782.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13332..13691)
                     /gene="yneG"
                     /locus_tag="ACHJ1G_001922"
     CDS             complement(13332..13691)
                     /gene="yneG"
                     /locus_tag="ACHJ1G_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4763783.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13691..14617)
                     /gene="glsB"
                     /locus_tag="ACHJ1G_001923"
     CDS             complement(13691..14617)
                     /gene="glsB"
                     /locus_tag="ACHJ1G_001923"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4763784.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14681..16069)
                     /gene="sad"
                     /locus_tag="ACHJ1G_001924"
     CDS             complement(14681..16069)
                     /gene="sad"
                     /locus_tag="ACHJ1G_001924"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4763785.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16170..17051
                     /gene="yneJ"
                     /locus_tag="ACHJ1G_001925"
     CDS             16170..17051
                     /gene="yneJ"
                     /locus_tag="ACHJ1G_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4763786.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17129..18243
                     /gene="yneK"
                     /locus_tag="ACHJ1G_001926"
                     /pseudo
     CDS             17129..18243
                     /gene="yneK"
                     /locus_tag="ACHJ1G_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18393..19583
                     /gene="ydeA"
                     /locus_tag="ACHJ1G_001927"
     CDS             18393..19583
                     /gene="ydeA"
                     /locus_tag="ACHJ1G_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4763787.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(19608..20273)
                     /gene="marC"
                     /locus_tag="ACHJ1G_001928"
     CDS             complement(19608..20273)
                     /gene="marC"
                     /locus_tag="ACHJ1G_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4763788.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20485..20919
                     /gene="marR"
                     /locus_tag="ACHJ1G_001929"
     CDS             20485..20919
                     /gene="marR"
                     /locus_tag="ACHJ1G_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4763789.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            20939..21322
                     /gene="marA"
                     /locus_tag="ACHJ1G_001930"
     CDS             20939..21322
                     /gene="marA"
                     /locus_tag="ACHJ1G_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4763790.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21354..21572
                     /gene="marB"
                     /locus_tag="ACHJ1G_001931"
     CDS             21354..21572
                     /gene="marB"
                     /locus_tag="ACHJ1G_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4763791.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(21629..23068)
                     /locus_tag="ACHJ1G_001932"
     CDS             complement(21629..23068)
                     /locus_tag="ACHJ1G_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4763792.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23093..24766)
                     /locus_tag="ACHJ1G_001933"
     CDS             complement(23093..24766)
                     /locus_tag="ACHJ1G_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4763793.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(24822..25133)
                     /locus_tag="ACHJ1G_001934"
     CDS             complement(24822..25133)
                     /locus_tag="ACHJ1G_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO4763794.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25161..26483)
                     /locus_tag="ACHJ1G_001935"
     CDS             complement(25161..26483)
                     /locus_tag="ACHJ1G_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4763795.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(26598..26909)
                     /locus_tag="ACHJ1G_001936"
     CDS             complement(26598..26909)
                     /locus_tag="ACHJ1G_001936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4763796.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27108..27806
                     /locus_tag="ACHJ1G_001937"
     CDS             27108..27806
                     /locus_tag="ACHJ1G_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4763797.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(27851..28750)
                     /gene="eamA"
                     /locus_tag="ACHJ1G_001938"
     CDS             complement(27851..28750)
                     /gene="eamA"
                     /locus_tag="ACHJ1G_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4763798.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            28945..30132
                     /gene="ydeE"
                     /locus_tag="ACHJ1G_001939"
     CDS             28945..30132
                     /gene="ydeE"
                     /locus_tag="ACHJ1G_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4763799.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            30259..30354
                     /gene="mgtS"
                     /locus_tag="ACHJ1G_001940"
     CDS             30259..30354
                     /gene="mgtS"
                     /locus_tag="ACHJ1G_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4763800.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            30354..30458
                     /gene="mgtT"
                     /locus_tag="ACHJ1G_001941"
     CDS             30354..30458
                     /gene="mgtT"
                     /locus_tag="ACHJ1G_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4763801.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(30573..>30859)
                     /gene="dgcZ"
                     /locus_tag="ACHJ1G_001942"
     CDS             complement(30573..>30859)
                     /gene="dgcZ"
                     /locus_tag="ACHJ1G_001942"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4763802.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         8041 a         7659 c         7181 g         7978 t
ORIGIN      
        1 ctttaagaaa aatagcaatt ttcccttgaa tatagtactg gtgatggaac gatgaatctg
       61 caagctggca tttaacaagc cagctctaaa agaagggaaa taagaaaaac tatactcaaa
      121 aactaacagc cacggtcatc atgatgtggc cgtcaatgaa actataaccc aggcgctttc
      181 cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc
      241 cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt
      301 tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg
      361 ccagctgcaa tggcacatcc taatgcggtg gcttctttga ctaccggaat attgacgggt
      421 aatcccgaga catcagcgag aatttgactc cataatttcc cttttgaacc tccgccagca
      481 aaaaccagcg aggtgggatg aatattcgag aaatcagcaa tttgctgcaa gttacacgct
      541 gatacaatcg ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc
      601 gggtcaatgg acaagttaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt
      661 ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct
      721 tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag
      781 aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt
      841 accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc
      901 gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt ttgtgccgga
      961 cgcacaacgc ctaacccaag gcaaccaagc tgcacgtcgc ctcctccaac gaccaccgga
     1021 gtgcccgcct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg
     1081 ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc cagcaatgca
     1141 ggtttccagt cacgggtggt tagatcaaag tcccgtggtg ccagcgttag agggatccac
     1201 cgccagttcg ccgctgagca tataggccag ccagtcgctg atcatggtga tggttgatgc
     1261 ctgacggtaa atatcggaac gatggtgcgc cagccaaagt aatctgggga tggcacttaa
     1321 agccagtgtt tgtccggtcg cgcgataaac ttcgttttca aaggtattgt tgtgcagttc
     1381 tttaagttcg ctaacttcgc gcgctgctct ggcatccaca ttggcgcagg cccagatcgg
     1441 tgttccttca ttgttatata aaacaatgcc ttcacgcata gaacatgccg aaacggcagc
     1501 gatatactcc gggtctatgc cggcgttgtg cagcgcctgg cgcatacact cacacgccag
     1561 ttgccagttt ttgttgagat caaattccat agaaccagga acgtccggta ctgccagatg
     1621 ccgccactcc gcctgtccca ctgctatttg attgcctttc aggtcgaaaa tcacagcccg
     1681 aatacttccg gtgcctgcat ccagcgccaa caggtaatac tttgattctg aaggggtaaa
     1741 gagtcgagcc attagattat cctcggctta tgcgttctgt ctgttcctct atacgttctc
     1801 catcattccc ggtaataagg tcatgcaaat ttaactacgt aaaatcgccg ctgctgtatc
     1861 ctgatcggta accagtgcgt tgatataacc gcctttcatt gcagcggcaa ttgcttcggc
     1921 tttattttct ccccctgcca cgccaacccg gacgggtatg gtcttcagcg cgcttaaagg
     1981 taagccaatc agttcgttat gtattttaat atccgtgaca acgtcacctt ttgcatcaaa
     2041 aaagtagcct aaaatgtcgc caaccgcccc ttttcgacca atcattaact gttcgccctg
     2101 gctgatataa ccggagcgaa tgatcgtcgc gtcgtcctgt tgactgacgg ccccaatgcc
     2161 gacaatcgcc acatccgctg cttgcgcggc taacagaaca tctttgacgc aattttcatt
     2221 ttttagcgta cgggcaatgt cagcggagga tgcccgcaac ggagccggaa taatattcac
     2281 actgcacgcc gcgttaagct gcccgatacc cgtcatataa gaaccgacgc caccggagag
     2341 cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt aagcgttgca gcgtattcat
     2401 ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt tgaagtaaac tcatcaacat
     2461 atgcgccgcg cctatcccta gtcgcccgcc gacatcagca tccgcaagcc cagggatcac
     2521 ccggacatgt tgcagcgaaa actgacgacg taattgagtt tcatattcca gacagccttc
     2581 aaagcgagaa ttaatctgta cgcgaataat gccggactga tgccctttct ccagcaagcg
     2641 cgacactttc aaacgggtca ggccgagacg atcgctgatc tcgctctggg tcagcccgtc
     2701 gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct tcacacattc cctgttctga
     2761 aattgccgaa tcgttgattg tcataattca ttcttcactt tgaacatatt taaatcttta
     2821 atgcaattgt tcagttcttg cgcatttata tctgtgatgg cagccacagt ttgactctac
     2881 gagcatgaac aaacgcaacc gtgaaaatca aaatagcata aattgtgatc tattcgtcgg
     2941 aaatatgtgc aatgtccacc taaggttatg aacaaattaa aagcagaaat acatttgttc
     3001 aaaactcacc tgcaaaactg aacgggggaa atatgcaaac gagtgatacc cgcgcgttac
     3061 cgctactttg cgcccgctcg gtttataaac agtattcagg ggtcaatgtc ctgaaaggca
     3121 tcgattttac gttgcatcag ggggaggtcc acgccctgct cggcggcaat ggtgccggta
     3181 aatcgacgtt aatgaagatt attgccggta ttacccctgc tgatagcggt acgctggaga
     3241 ttgggggcaa caactacgcc agattaacgc cagttcatgc tcatcagctg ggcatttatc
     3301 tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat aaaagaaaac atcctgtttg
     3361 ggatggcaaa aaaacagctc tccatgcaaa aaatgaagaa cttgctggcg gcgctgggct
     3421 gccagtttga tctgcatagt ctggcaggat cgctggatgt cgccgatcgc caaatggtgg
     3481 aaatcctccg cgggctaatg cgcgactcgc ggattctgat cctcgatgaa cctaccgcct
     3541 cgcttacccc tgcggaaacc gaacgcttgt ttactcgctt gcaagagctg cttgctactg
     3601 gcgtgggtat tgtttttatc tcgcataagc tgccggaaat tcgccagatt gccgatcgaa
     3661 ttagcgtgat gcgcgacgga accatcgcct taagcggcaa aaccagcgaa ctgtctaccg
     3721 acgacattat tcaggccatc acgccagtgg tacgggaaaa atcgctctct gccagccaaa
     3781 aattatggct ggagttacct ggtaaccgcc cacaacatgc tgccggaacg ccggtgctga
     3841 cactggaaaa tctgaccggc gaaggtttca ggaatgtcag catgacgctc aatgccggag
     3901 aaattctggg cctggctggg ctggtggggg ccggacgcac agaactggcc gagacgttct
     3961 atggtctgcg tactttgcgt agcggacgca ttatgctgaa tggtaaagag atcaataaat
     4021 tatccactgg agaacgttta ctgcgcggtc tggtttatct gccggaagat cgccagtcat
     4081 ccggactgaa tctcgatgct tcgctggcct ggaacgtctg cgcccttact cataaccttc
     4141 gtggattctg ggcgaaaacc gcgaaagata atgccaccct ggaacgttat cgtcgggcgc
     4201 tgaatattaa atttaaccaa ccggaacaag ctgcacggac attatccggt ggcaaccagc
     4261 aaaaaatcct cattgccaaa tgcctggaag cttcgccgca agcattgatt gtcgatgagc
     4321 cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta ccagctgttg cgcagcatct
     4381 ccgcacagaa tgtggctgtg ctgcttatct cctctgatct ggaagagatc gaactgatga
     4441 cagatcgtgt gtatgtgatg catcagggcg aaattaccca ctctgcactg accgggcgcg
     4501 atattaatgt cgagactatt atgcgcgttg ccttcggcga tagtcagcgt caggaggcat
     4561 catgctgaag tttattcaga acaaccgtga aatcacggca ttgctggcgg tggtgctgct
     4621 gtttgtatta cccggttttc tcgaccgtca gtatttaagt gtgcaaacgc tgaccatggt
     4681 ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc gcgacactgg taatgcttac
     4741 gcgcaatatt gatgtttcag tgggttcgat taccggaatg tgcgcggtgc tgttggggat
     4801 gttactgaac gcaggatatt cactacctgt tgcctgtgtc gcgactttac tgcttggttt
     4861 gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta aagatccctg ccattgttgc
     4921 cacccttggc acgttagggt tgtacagagg catcatgttg ctgtggactg gcggtaaatg
     4981 gattgaaggg ttacccgccg aactgaaaca gctctccgca ccgctgctgt ttggcgtttc
     5041 cgcgattggt tggttgacga taattctggt ggcatttatg gcctggctgc tggcaaagac
     5101 ggcgtttgga cgcagttttt atgccacggg cgataattta cagggcgctc gtcaactggg
     5161 tgttcgtacg gaagccattc gcattgtggc attttcgttg aacggctgca tggcggcact
     5221 ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc aaccagaccg gtaccgggct
     5281 ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt agtttgctcg gcggttccgg
     5341 tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg cagatcgata gcgtactggt
     5401 gctgttgcgc attccggcat ggtggaacga ttttatcgcg ggcctcgtgc tgctggcggt
     5461 gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt aatctacggc ggcaaaaata
     5521 tgcccgcttt atgacgccac cgccatccgt taaacccgct tcgtcaggta aaaaacggga
     5581 ggccgcataa tgcgtattcg ctacggttgg gaactggctc ttgccgcact gctcgttatt
     5641 gagattgtcg catttggtgc aattaacccg cgaatgttag atctcaatat gttgctgttc
     5701 agcaccagtg actttatctg cattggcatt gtcgccctac cgctgacgat ggtgattgtc
     5761 agtggcggga tcgatatttc gtttggttcg accatcggcc tctgcgccat tgcattgggc
     5821 gtactgtttc aaagtggtgt gccgatgccg ctggcgatac tcctgacctt actgctcggc
     5881 gcattgtgcg ggctgatcaa cgccggatta attatctata ccaaagttaa cccgctggtg
     5941 attacgcttg gcacgctgta tctgtttgcc ggaagcgctc tgctgctttc cggtatggcc
     6001 ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg cgtttacaga tttcgctaac
     6061 ctggatgtgc tgggactccc cgttccgctg attatcttcc tgatatgtct cctcgttttc
     6121 tggctctggc tgcataaaac ccatgccgga cgtaatgtgt ttttgattgg gcaaagcccg
     6181 cgcgtggcgc tttatagcgc gattccagtt aaccgcacct tatgtgcgct ctatgccatg
     6241 acggggctgg cgtctgcggt cgccgctgtg ctgctggtat cgtattttgg ttcagcacgt
     6301 tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg ccgtggtgct tggcggggcc
     6361 aatatttatg gtggttccgg ttccattatc ggcaccgcca ttgcggtttt attagtggga
     6421 tatttgcagc aaggtttgca aatggcagga gtgccaaatc aggtgtccag cgccctttcc
     6481 ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta gcctgcatcg ccagcaaatt
     6541 aaagagtggc tggcgcgtcg ggccaataac ccattgccat aaaggatatc ttcatgacac
     6601 ttcatcgctt taagaaaatc gccttactta gcgctcttgg cattgccgca atctctatga
     6661 atgtgcaggc cgcagagcgt attgcattta ttcccaaact ggttggcgtg ggatttttta
     6721 ccagcggtgg caacggcgca caacaagcgg gtaaagagct gggcgttgat gtgacctacg
     6781 acgggccgac agaacccagt gtttctggtc aggtacagtt gattaataac ttcgtcaatc
     6841 aaggttataa cgccattatc gtttctgcgg tttcgcctga tggcttgtgt ccggcactga
     6901 aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgatact aaaccggagt
     6961 gccgctctta ctacattaat cagggaacgc ccgcccagtt gggaggtatg ttggtggata
     7021 tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac tcaagcccca
     7081 ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc aaagagcatc
     7141 ctggctggga aattgtcact acgcagtttg gctataacga tgccactaaa tcattacaaa
     7201 ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc cccgacgcca
     7261 acgccctgcc cgctgccgca caagccgcag aaaacctgaa aaatgacaaa gttgcgattg
     7321 tcggattcag tacgccaaac gtgatgcgtc catatgtgga acgcggcacg gtgaaagaat
     7381 ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt gtatgtcgcg gatgcattat
     7441 tgaaaaaagg atcaatgaaa acgggcgaca agctggatat ccagggcgta ggtcaggtgg
     7501 aagtctcgcc aaacagcgtt cagggctatg actacgaagc ggatggtaat ggcatcgtac
     7561 tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat ttctgatgtg
     7621 cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg gtaaagattt
     7681 tcgtaccgat caatcgcaac aaaatatccc ttttatcctg aaaggttgcg gtgcgctgga
     7741 ttggggaatg cagtcacgct tatcgcggat atttaatccg aaaacgggta aaaccgtgat
     7801 gctggccttt gaccatggtt attttcaggg gccgactacc ggacttgaac gcattgatat
     7861 aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg gcattttgcg
     7921 cagcgtagtt cccccagcga ccaataagcc ggtggtactg cgggcgtcag gagcgaactc
     7981 tattctggcg gaattaagta atgaagccgt ggcgttatcg atggatgatg ccgtgcgcct
     8041 gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac atcagtcgat
     8101 caaaaatatt attcagctgg ttgatgccgg aatgaaagtg ggaatgccga ccatggccgt
     8161 gactggcgtg ggcaaagata tggtgcgcga tcagcgttat ttctcgctcg cgactcgaat
     8221 cgccgctgaa atgggggcgc aaattatcaa aacctattat gtcgaaaaag gttttgaacg
     8281 gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat taccggagcg
     8341 cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg tggatatggg
     8401 gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac aggcggtggt
     8461 tcaccataac gaaacggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     8521 tgcaatggct gcccgaccac tacgaattgt ttcctcatct ggtttcttta cttaatccgc
     8581 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     8641 gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     8701 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     8761 actatcacca gatgccgtcg cactaggcga ttatcgattg ggtgaccgcc actggattac
     8821 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     8881 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     8941 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     9001 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     9061 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     9121 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     9181 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     9241 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     9301 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa ctggatatct
     9361 gaaagctgcc cattgcggcg ctgaaccgcc aaccactccc ccattattaa caagatacgg
     9421 ttagctggag agttcgaagc cagtacaggc tgaagatctt cagtcttgag ataatgagcc
     9481 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     9541 tgcaaccggg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc
     9601 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     9661 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     9721 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca
     9781 gcgatagaaa agagaaaatt gagtagtaag cgcgatgata tcttggataa tactgagccg
     9841 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     9901 aggcgtgaaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     9961 caaattacct acaagcttac aacattaaca ggcgctgcat gtggcatctg ttaatgataa
    10021 aggcacatag gatgttgtaa ctaatatagt gaaataagaa acccggtcga aaccgggttc
    10081 agaagtaacg gtgttattag cacaacggac gtacagcttc gcgcatccct ttttcaagaa
    10141 tcgcatccag gtcattagca acctgctcga ccagaccagg cacttgcgtc aggtcctgct
    10201 cccagtggtc tttttctgcc aacacaatcg ctaccagttc ctgggtaccg ataacacggt
    10261 cacgatgctg gctccagagt tgctgataac gttccagcca gtgtgcatcg tcctgtaccg
    10321 gatatgtttc tccgttacgc tcaccacgat agaaggcaat caatgccgct aatgcgaaag
    10381 taaggcgcgc cggaagtgtg ccttttgcct tctgccgtgc cagcagctgc ggcaggatgc
    10441 gagtgcggaa cttggtcata ccgttgagcg cgatagacag cagctgatgc ttaatgtacg
    10501 ggttacggaa acgcccggtg actgcactgg cgaaagattc cagttcatca cgaggcaaat
    10561 ccagtaccgg aataatttct tcgtagatag ctttttcaac gaatgcgcaa atttcagcat
    10621 cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa cgccaccggc accagcgcgg
    10681 tgtgcgcacc gttgaggatt gccactttgc gttctttgta cggtttaata tcgtcaacaa
    10741 tcagcacgtt gagcggatat ttgtccagac gcagttcagt cgctaaggat ttcggtccct
    10801 gaatcacaaa caggtaaaag tgttcagcgg tgtcgagaaa accatcgtga taacccagct
    10861 cttcttccag tttagccact tcatcgcgcg gataaccggt aacgatacgg tccaccagcg
    10921 tagaacagaa gctgttagcc tgatccagcc attgaataaa tgcttctggc agtgcccact
    10981 cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc attgtagtcg atcaactcac
    11041 acgggatgat gatccaacct ttatccagcg caccgttgaa atggctaaaa cgttcgaaca
    11101 gcagacgggt cagttttgcc ggataactta ccgctggcgc gtcatcgaat ttatcgcccg
    11161 catggtagct gatacccgct tcggtggtat tagagaaaac aaagcgcatt tccgggttgt
    11221 gagccagttt caggaattca tcgtattcac tgtagacgct gatttcacga ttaaccgagc
    11281 gaatcagacg cgcgtcgctg accgcttccc ccttctcatt caggccacgg ataatggtgg
    11341 tatacagacc atcctgcgtg ctcagtgacg gtgggaatga agtttcaatc ggacgaacaa
    11401 cgaccacacc agaattcaga tcggtgtgct cattcaggag atcgatttgc cagtcaacaa
    11461 aggcgcgcag gaagttacct tcaccaaact gaatgatacg ttctggatac tgtgcaccgg
    11521 gaaaatcgcg acgatttagt gttttcacaa tgggttccct tctgattagt catacaacct
    11581 gtttgaattg gtacgacagg ttagcaaact ttaatacgcc gaacccctgt tttgatcaac
    11641 tcctgatgat taatgagcag ttttatgaga aaagtgtggc gcggatcatg gtttaatcga
    11701 ggaaatacgc cttttcctgg atcataaagt ggtagaacac attgcattca aatcgcgcgg
    11761 aatgaataaa gatgtcagac aacttcctca ccgtaacgca tagtgctggt acggttgcgc
    11821 ccatctttct tcgagcgata cagacacgta tcggcttcga ccatcaattt attaaaatca
    11881 tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa
    11941 agcgtttttt gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc
    12001 attagcagac catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc
    12061 atgcgcgcca ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt
    12121 aacactttat cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata
    12181 tcaagcagca tgaccgtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca
    12241 taaagaccgg accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact
    12301 tgcttcatta gcgaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc
    12361 atcgtggcaa taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc
    12421 gagatatcaa tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc
    12481 aacagacatg ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg
    12541 aacgccagac tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg
    12601 atggcgggca tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc
    12661 agcatacaag gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc
    12721 aaaggccaga aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat
    12781 aataatgcgg caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta
    12841 tttttcccca gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata
    12901 ttggagaaat tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc
    12961 agcatcgcca cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca
    13021 aacacccccg ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt
    13081 aactctgcac caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt
    13141 agcagatgac cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat
    13201 ctgtccatac aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga
    13261 tagcatgttc gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt
    13321 ttccgcaatt ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg
    13381 atataacgtt gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc
    13441 agacaaccgc gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc
    13501 atgggggttt gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa
    13561 tccgccgcat gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc
    13621 atgcccagac gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg
    13681 agagactgca ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcaa
    13741 tacccgcaag cgagttacct gcatcatcca gttccggact ccagacagcg atggccattt
    13801 catgcggaac aatcgccaca ataccgccac caacgccaga tttcgccggt agccccaccc
    13861 gccaggcaaa ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt
    13921 gccgcgcctg cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag
    13981 ccagaaagac aaacgtcctg gccagctcta cacagctcat tttcagagcg cagtaatgaa
    14041 agtagttttg cagaacggtt gtcacatcat gatggaaatt gccaaacgac ttcatcagcc
    14101 aggcgatagc cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt
    14161 atgaaatatc agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg
    14221 cgcttaatcg cccttgcaac atatcgcaaa ccaccagcgc accagcatta ataaacggat
    14281 tacgcggaat accctgctcc atttccagtt gcactaaaga attgaacggt gatccagacg
    14341 gatctttgcc gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga
    14401 gactcagcac tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa
    14461 aaagctgtcc gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca
    14521 gcgccggaat ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga
    14581 tgttttctaa aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc
    14641 tgaaaagacc tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca
    14701 ccgtctggat attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac
    14761 tctttttcac gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca
    14821 ccccaccgca ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa
    14881 tggtcgctga aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt
    14941 tcgcaacggt gattgccgca acagggccaa acatttcttc ccgaaacgcg gtcatttctg
    15001 gggtaacatt cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc
    15061 cgcccagtaa caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat
    15121 cacgtaaatc aaaacgagcc attggtccga gagcattctc ttcatcacgg ggatcgccca
    15181 ttttcaaggc tgccgcagct gccacaaaac gttcggtaaa tgcagaagca attccctctt
    15241 cgataataaa gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta
    15301 ccgccgcttt aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac
    15361 cgcccagttc cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc
    15421 ccgcacgaac acttccggtc accgtgacag cagcaatgcg cgaatcttta atcatttgac
    15481 tgacaccgtc gttgtcggca ttcagccagc catatacgcc ttgcgggatt cccgcatctt
    15541 taaacacctg ggcaatgagc tgagcacagc ccatcacatt cggcgcatgt ttaagtaagt
    15601 agccgttacc tgcaagaatg atgggaaccg cgccacgcat cacctgccat aacggaaagt
    15661 tccacggcat aatcgccaga atcgtcccca acggtcgata ctcaataact gcctgctgat
    15721 tttccaccag cgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac
    15781 acaaattcgc cgatttcgcc acttcagcgc gcgcctgatt gattggtttg cccatttcac
    15841 gggtgatcat ttgcgccatt tcttcgctac gggcgcgcag agccttaccg ataccacgca
    15901 gtttttcagc acgataatct atatttgtct cgcgccagtc gcgaaagcct gctgccgcca
    15961 gctgaagtgc gttttcgata tcgttagcgc cagcccacgg cagcacagaa agttgttcac
    16021 ccgtggcagg atttattgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc
    16081 ctttatgagt catggtatga agatacgcag atttactctt gctttaaaat gaataatatt
    16141 aagccactta ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg
    16201 cggttgccga agctggcagc attacccagg ctgcagcaaa agtgcatcgc gtaccgtcga
    16261 atctcactac ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg
    16321 agaatcagcg tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa
    16381 ttctcgcgtt agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat
    16441 tttctcttgg ttcgctggaa agcaccgcag cagtgcgcat tccggccacg ctggcggaat
    16501 tcaaccatcg ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc
    16561 tggaaggagt gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata
    16621 ctgccattga cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc
    16681 atgcgccagt aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcgcca
    16741 attgttcgta tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa
    16801 ctattcacga gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca
    16861 ttgcgcttat tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt
    16921 ggccgttagc tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgcggtgcga
    16981 aaacgcgtcc gcttgaggca tttattcaac tactggatgt gcctgactcg gcaaaacagg
    17041 gttaccaatg agctattttt gatagttctt gcagctcaaa tgccctatat agtgatgttc
    17101 cgatgactta tgactatatg gggcaaatat ggttacgcca gtaagcatca gtaattacat
    17161 atctcttcct gatgattttc ccgtacgtaa tattgcacct caggtaaaag aagttttaaa
    17221 ggactttatt gatgcgctaa gtacaataat atgtgatgaa gagtggcgca cgtctttaaa
    17281 catcaactca gccacaaaaa agatatttaa taaccttgat aacttatcat atattcagag
    17341 gacttctttt cggggtaacg acacgctata caatgaaaag gttcagttta aactcactta
    17401 tcccgtcaag aatggaagac acaaagaaaa tattgaattt caggtagtaa taaatttaag
    17461 ccctatttat ttagataatt ttcgccatga tggagaaatt aatatttttg cgctcccaac
    17521 ccaaagcctg tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt gctatttctg
    17581 tttctgaatg attttattga acaatttcca atgatcaacc ttggcgttcc catcaaaaga
    17641 gcgcatacac cacatattga acccctgcct ccggatcgcc ataccgctgc agattactta
    17701 cgccagtttg atttgcttgt cctgaatttt atctctcgcg gtaattttgt catactcccc
    17761 cgattatgga gtaactctga ggttcacaga tggttcgtca ataaagatcc taacttgatc
    17821 accgccattc tcgacataac agacggcgaa ttaaaagagg atttattgca aagcctgatg
    17881 gattcattag gttctaacaa acatgtacta cccgaagtct gcatctgctt tttatccctt
    17941 ttagcagaac aagaatctcc tcattttcag gacttttttt tatttttcgc caatatgtta
    18001 ttgcactatc accaatttat gaatcccaat gaaagtgatc tgaatgacgt gttgatgcca
    18061 gcatcattaa gtgatgataa aattatcaaa catatggcgc gcaggaccct caaactgttt
    18121 gtaaaaaatg aaacgccgcc aaaagttact cacgaagacc tggtgaaaaa caggcctcgc
    18181 tcccctgtca gaccacctat acccgcaacc gccaaaacgc cagacctccc tgaacgtcat
    18241 taaaccgtga tgttaccgac tctctgacgc ggaacagaat cagcgtcaga gaaacggaaa
    18301 acgcgatcca gatcacaaat gcattgtatt cacatcatta accgttttaa gatcatttca
    18361 tcactttttc gcaactcacc cgataatctg ttatgacaac aaacactgtt tcccgcaaag
    18421 tggcgtggct acgggtcgtt acgctggcag tcgccgcctt catcttcaac accaccgaat
    18481 ttgtccctgt tggcctgctc tctgacattg cgcaaagttt tcacatgcaa accgctcagg
    18541 tcggcattat gttgaccatt tatgcatggg tagtggcgct aatgtcattg ccttttatgt
    18601 taatgaccag ccaggttgaa cggcgcaaat tactgatctg cctgtttgtg gtgtttattg
    18661 ccagccacgt actgtcgttt ttatcgtgga gctttaccgt tctggtgatc agtcgcattg
    18721 gtgtggcttt tgcacatgcg attttctggt cgattacggc gtctctggcg atccgtatgg
    18781 ctccggccgg gaagcgagca caggcattga gtttaattgc caccggtaca gcactggcga
    18841 tggtcttagg tttacctctc gggcgcattg tgggccagta tttcggttgg cgaatgacct
    18901 tcttcgcgat tggtattggg gcgcttatca cccttttgtg cctgattaag ttacttccct
    18961 tactgcccag tgagcattcc ggttcactga aaagcctccc gctattgttc cgccgcccgg
    19021 cattgatgag catttatttg ttaactgtgg tggttgtcac cgcccattac acggcataca
    19081 gctatatcga gccttttgta caaaacattg cgggattcag cgccaacttt gccacggcat
    19141 tactgttatt actcggtggt gcgggcatta ttggcagcgt gattttcggt aaactgggta
    19201 atcagtatgc gtctgcgttg gtgagtacgg cgattgcgct gttgctggtg tgcctggcat
    19261 tgctgttacc tgcggcgaac agtgaaatac acctcggggt gctgagtatt ttctggggga
    19321 tcgcgatgat gatcatcggg cttggtatgc aggttaaagt gctggcgctg gcaccagatg
    19381 ctaccgacgt cgcgatggcg ctattctccg ggatatttaa tattggaatc ggggcgggtg
    19441 cgttggtagg taatcaggtg agtttgcact ggtcaatgtc gatgattggt tatgtgggcg
    19501 cggtgcctgc ttttgccgcg ttaatttggt caatcattat atttcgccgc tggccagtga
    19561 cactcgaaga acagacgcaa tagttgaaag gcccattcgg gcctttttta atggtacgtt
    19621 ttaatgattt ccaggatgcc gttaataata aactgcacac ccatacatac cagcaggaat
    19681 cccatcagac gggagatcgc ttcaatgcca cccttgccca ccagccgcat aattgcgccg
    19741 gagctgcgta ggcttcccca caaaataacc gccaccagga aaaagatcag cggcggcgca
    19801 accatcagta cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc cgagctaata
    19861 atcatcgcta tggttcccgg accagcggta cttggcattg ctagcggcac aaaggcaata
    19921 ttggcactgg gttcatcttc cagctcttcc gacttgcttt tcgcctccgg tgaatcaatc
    19981 gctttctgtt gcggaaagag catccgaaaa ccgataaacg cgacgattaa gccgcctgca
    20041 attcgcagac cgggaatcga aatgccaaat gtatccatca ccagttgccc ggcgtaatac
    20101 gccaccatca tgatggcaaa tacgtacacc gaggccatca acgactgacg attacgttcg
    20161 gcactgttca tgttgcctgc caggccaaga aataacgcga cagttgttaa tgggttagct
    20221 aacggcagta acaccaccag ccccaggcca attgctttaa acaaatctaa cattggtggt
    20281 tgttatcctg tgtatctggg ttatcagcga aaagtataag gggtaaacaa ggataaagtg
    20341 tcactcttta gctaaccttg catcgcattg accaaaactt gaaccgattt agcaaaacgt
    20401 ggcatcggtc aattcattta tttgacttat acttgcctgg gcaatattat cccctgcaac
    20461 taattacttg ccagggcaac taatgtgaaa agtaccagcg atctgttcaa tgaaattatt
    20521 ccattgggtc gcttaatcca tatggttaat cagaagaaag atcgtctgct taacgagtat
    20581 ctgtctccgc tggatattac cgcggcacag tttaaggtgc tctgctctat ccgctgcgcg
    20641 gcgtgtatta ctccggttga actgaaaaaa gtgttgtctg tcgaccttgg agcactgacc
    20701 cgtatgctgg atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa cccgaatgat
    20761 aagcgcggcg tactggtaaa acttaccacc agcggcgcgg caatatgcga acaatgccat
    20821 caattagttg gccaggacct gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtg
    20881 gcaacacttg agcatttgct taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat
    20941 gtccagacgc aatactgacg ctattaccat tcatagcatt ttggactgga tcgaggacaa
    21001 cctggaatcg ccattgtcac tggagaaagt gtcagagcgt tcaggttact ctaaatggca
    21061 cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta ggccaataca tccgcagccg
    21121 taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac tctatctggc
    21181 agaacgatat ggcttcgagt cgcaacaaac tctgacccga accttcaaaa attactttga
    21241 tgttccgccg cataaatacc ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc
    21301 attaaatcat tacaacagct agttgataac gtgacaacgt cactgaggca atcatgaaac
    21361 cactttcatc cgcaatagca gctgcgctta ttctcttttc cgcgcagggc gttgcggaac
    21421 aaaccacgca gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc ccatcgcagg
    21481 aacaaccacc gtttgattta aatcacatgg gtactggcag tgataagtcg gatgcgctcg
    21541 gcgtgcccta ttataatcaa cacgctatgt agttattgtc aggctctgat cacagagcct
    21601 gacatctccg taatatcttc ctcaagtctc aaatattttc gccattgttg gcgatgagat
    21661 cccgatacca gtaaaacgac gctttacgta tccgttccag cgttcccttc ccttcgttgt
    21721 ctttatcgac ataaatcatt ccgtagcgct ttttcatttc ccctgttccg gcagaaacca
    21781 ggtcaatgca gccccacggg gtgtagccaa ttaagtcaac accatcttca actacggctt
    21841 ttttcatttc ccgaatatgg gaagcaaagt aatcaatgcg atagtgatcg ttcaccgtgc
    21901 cgtcagcttg tctctggtca accgcaccaa atccattttc gacaataaac agcggcaact
    21961 ggaaatgatc ccagaaccag ttgagtgaat aacgtagccc tgccggatca atttgccatc
    22021 cccattcgga tgtatcgata taagggttgc tgaccaaatc tcgtggttca acataatcca
    22081 gttgcgggtt atcgggcgaa aattgtgtcg taaaagacat gtagtagcta aagccgataa
    22141 agtcgacaca accactggca agaatcgcgt tatcttctgg tgtgatatcg aggttgaatc
    22201 ctttcctggc aaagtaattc agcatatgtt gcggataata tccacgagca tgaacatcag
    22261 taaaccagta acgacgatgc attgctttcg tggccatcat catatcgttg ggtgcacacg
    22321 tcagaggata gatgggacac atggcgatca tacagccaat gttaaattca ggattgatcg
    22381 attttccagt ctgtaccgcc agggcactgg caactaactc gtaatgtacc gcctggtaca
    22441 ttatttgttc gcgctcattg atatcttcct ctggcgaata caagataccg gaattagtaa
    22501 atggacacag gctttcgctg aaattcacct gattattgat ttcgttaaac gtcatccagt
    22561 actttacttt ttctttatag cgcgtgaaga ccgttgatgc gaagcggata aaaaagtcga
    22621 tcagtttacg gtttcgccag ccaccatatt ttgtcaccag atgataaggc atctcaaaat
    22681 gtgaaagcgt caccacaggt tccattccct gcttaaggca ttcatcgaac agatcatcat
    22741 aaaattgtaa accctcttca ttcggctcct gttcgtcacc ttgcggaaag attcgtgtcc
    22801 aggcaatgga agttcgaaag catttgaatc ccatctcggc aaataactga atatctgttt
    22861 tatagcgatg ataaaaatca attgcttcat gattgggata attaagcccg tcgataacgc
    22921 cttctgtcac ttcacgcggc accccgtgag cgccagcagt catcacatca gcgatgctga
    22981 tgccttttcc tccttcattc cagccacctt ccaactgatg cgcggctacc gcaccgcccc
    23041 ataaaaaacc ttttttaaat cctgacatac acaactcctt aactaaaata gattaccacc
    23101 agacttccgc ctggatgccg gccataaact cgtctttgct gttctcatta aacttaaaat
    23161 tcgataattc gttatccaga atattgatat acgttccata gaagcgaatt tctggtcgtg
    23221 aacctaaaat gctttcaccc actttcaatg catgccagag tgtcgtttta taagccgatt
    23281 catttagtgt tacaccctgc tgagttttgt tctgttgctt aaaccagcct aattcaagcc
    23341 ccgtctgatt ccatgtattc cagatccagg ccggtcttat tacagtgcga atactgtcaa
    23401 aatcactatg agcaccactt tcataactat aaacatcttc gccgtgagaa tagacaagcg
    23461 cgttagccat aataatattg tcagtaagat acatctcgcc ctgagagatt aaacgccagg
    23521 cgatcccatt ggtatgatca ccatagtaat agcgaccatg cgccatcgta ttactggcat
    23581 ctgagaaact ggcaaaactg ctggcatagg aattattggc aacctgtaac gtaaattcat
    23641 taaacgtatc gcgttgcagg ttttgccgta aaacagaagt cagcatccag ctatctttca
    23701 tttcaaaata actgttgtca ttttcgttat cttgttgttg atccgtttta ttaggtgcgg
    23761 aatatttagc cattaatgat aatgtcgcat catcccataa cgggatattg cgataacgaa
    23821 catccacaga attggtattc atctggctgg tacgcgaaaa atcacgagag taaacatcga
    23881 catcatcacg gcttaaggac atatcaaaca gccctacacc aagtgcccag ttttcaatcc
    23941 ccacacccgc ggcgacatcc gtggttaagg ttttccagtc cagcatctgg atctcatatt
    24001 gcgggagttt atgtttgccg acccagaagt ctgcctctgg tgcgaagggt aaaaaacctc
    24061 gcgttgtcag gtaaatatcg ctgaactgca tgatattttc attggcactg tcaccaaacc
    24121 aggcatcgtt atactgctca cccacgttgc catcataggt aacgaccgca tttgccgttt
    24181 taccgtcctg attataaaca cgctgattta aggtcaggtc aaaccaaccg ctcatctcat
    24241 taccaaaacg tcccagagaa cccgctgcat aagtttgtgg tgagccgtga ttaccagtac
    24301 cccagcctga gcggaagtac ccctgatagc tgaacccaat atcatccttt atatatttac
    24361 tgatatcttt cagggtcacg ctttcgatag tggttttgtc gcctttactt tcaataacgg
    24421 caacttgctg cgatccattt atagtatgcg ctgcggtaaa cgagttctgt tcaccgtttt
    24481 catttttgat gttatcagcg acgggagatg tcgctgatat ttcagcaagc gaggctgatt
    24541 tatatttgtt ttccgtgatg cttttttgta aggtcgaaag tcgggattta tatactccca
    24601 gttcattctg tgttgctttc agctcttgtt tattttgcga caattcattt tcaagcaatt
    24661 caaggcgctg ctctatggtt aattttgttg ccatcgcgtt caatggaaac aatgctgtaa
    24721 taatagaaaa agacaaaagg ctgacgttaa gcgtctttat attcatgact tatccttaag
    24781 agtataataa tttcctccag ctatcgcact ggaggaaaaa atcaggcatg gagttctttt
    24841 cttaatgaaa taatttccct ggctaaatcc tggcataata ttgcattcat caaatgatcc
    24901 tgtgcatgaa tcagaattaa attaactttt attttgccac agccttcatc ctgactaatt
    24961 aatgcggtct gaatcttatg tgcctgaaga caggcagatt cagaactggc cattagctga
    25021 gttgcgccct gccagtcttt ttgcccggca caacgtatgg cttccatcgc gtcagaacgt
    25081 gcctgccccg cgttgaccag taactccata acaagttctt catctgcaaa catcacaatc
    25141 tccttcctgg gtaaatggaa ttatccgtta gttgtcatgt tattgacttt gttggcggca
    25201 ataacaaaag gtaaatagat cattgtcgcc acacctaaac ataaaaaacc aaccagtaaa
    25261 gccagccagt tccccccggt tccgaggaaa gcaattaaag gtccgggcgt agtccagggc
    25321 acggcataag caaccggtgg aataatttcc atcgaaacaa agaagtaacc aatggcacag
    25381 ttaacaatgg gaaccataat aaacggcacc atcatgatgg ggttaagtac aataggaagg
    25441 tcaaacatta tcggttcatt gatattgaaa ataccgggga taaatgacat ttttgccaga
    25501 tcacggtatt ccgcacgctt agaagcgata aaaatagcca acaataaccc caacgtcata
    25561 cctgaaccgc cgcagttggc gaatgcatca ttaatagaag tccaggtaat cggatatggc
    25621 gcgccccagg tagtgccatg ttgagcggcc caggagagat tctccagatt agcctcagaa
    25681 aaaacggttt ctcgaattgc cgatgtggta ttaggtccgt ggatccccag cacccacagg
    25741 aaattaccca cagcccccag gataattacc gcaaagatat tcgttcccat atgtttgagc
    25801 ggcgtctgga ttaatgtgta aataagatcg tttagtcctg ccggggatat cagtgtcagg
    25861 caataattaa gtgcggaaaa gaacaccatg acaaaaaata ttggcaataa aactttaaat
    25921 gaacgcgcta ccgcaggcgg tacagctgcc ggcatcgtta tggtgatttt tgggtttcga
    25981 gctaagcgac aaaatatttc actggtgatc aatgcaacga taacagcaac aaacaacccc
    26041 tgcgcgccga gatatttggt cgtcaggaat gcttgcccat ctatcatctg atatggcgta
    26101 taaacaataa aaaatgctcc tattgccgtt aatccgcata aaagatcatc ttgctcatag
    26161 ctaatcgcca tattcctggc gaccaggaaa gcaatcataa ttgacatgat atttacagaa
    26221 ccattcatta ccggagtaaa aatagcttgt gctttttcaa ggttggggaa aatgctattc
    26281 agatgcagta atccggcaat aaaaccgtct ggcgataata tggcaaagtt aattaatata
    26341 attaatgaac ttgccatgat aattggaaac gtaaaaacga atccatctct caccgctgca
    26401 acatgttttt gtgagtttaa cttgatagca actggaacaa gaaaacgttc cattccacgt
    26461 tcgaatgagg ccattaatcc cattttttta tacctgttgt tttggatatt tatacatccc
    26521 aataaagcat gtatgccata tccattttat gatgtgtgaa aagttagttc aggtaatagt
    26581 ttaataatat tagattctta ctcttgcagt agtttcatcg caaattcaag aactgcctgt
    26641 ccgttcatgg tgccgtagtc tttcatctcg ataacggcta caggcatttt cccctgtgca
    26701 acggcttcaa tctctggttt ctgaaagcga acttgcgggc caagtaaaac aacgtcaact
    26761 tcgttatttt taattttccc tttagcctct gcaatcgcca atgcggaaat attaacttca
    26821 agtgaaatag cgttagcatg atcaatcata cgtttaacca gcatactggt tgacatgccc
    26881 gcagcgcaaa ctaacaatat ttttttcata ttttccctta ctggtatata acagattaca
    26941 ttgtttaaat atcatatccc gcagtagcta tacgtcctgg tacattgatc acagtaatac
    27001 agtgataaag ttgttcaaca tcagggtgat tattaaacga ctcggccata cgataaacca
    27061 atcaggactc aactccttgt tttcaatggc tcttaaaagg tgcaaaagtg atttttcaaa
    27121 agattgcccg cttacttaaa tccgaaatca atggcaattc atggcatgtt ggtgatttgt
    27181 tgccgtcaga agcggaactc gctgttcgtt ataatgtttc gcgtaatacc ctgcgtaagg
    27241 cattatcctt gctggaaggc gaaggaatta ttcacagaaa gcatggttca ggaagataca
    27301 ttcaaaaaaa gaattttgtt gcacacattg atcacatgaa cagtttcagt gaaattgcgc
    27361 ataaaagcgg caaagaggca gggagtcaga ttatgaaatt tgaagtgcag gatgcttcac
    27421 ccactattgc gactgagctg aatttagtga ctggtgagca ggtttattac ataaagagac
    27481 tgcgattcat tgaggataat gcagcacaat tagaagaaac gtggatgtca gtagcacgtt
    27541 ttcctgattt aaccgtgtca catatgcaaa aatccaaatt ttcatatatt gagaatgaat
    27601 gcgggatcaa aatcattggc acctttgaaa ctttctcccc tacttttcct accccagaaa
    27661 tcgccagtat tttacggatc agcccacggg atcccatact taaaattcag acccaggctg
    27721 tggacagtaa ctctattccg ctggattatt cgttacttta cagcaatatt ttcgagttcc
    27781 aggtaaagta ctttttcccg cgataaaagc agacgcacag agtgctgaca tcacattctg
    27841 tgcgtatggt ttagcctcta acctttaccg ctttacgcca ccgcaagcca aatacattga
    27901 tatacagccc ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat
    27961 ccaacaatag tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa
    28021 cccgccaggt ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg
    28081 ccacaaacgc cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt
    28141 gaatcatgga tgcggaacca tcgagaatca gcgaggcaac aaagaaggga atgattggga
    28201 ttaaagcgct ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct
    28261 ttttattgaa gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc
    28321 cgagcatcgc cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa
    28381 tcgctaaggc gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc
    28441 caagcacgat agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca
    28501 taccaaagtt aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat
    28561 accccaagag caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag
    28621 cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt
    28681 tgatgaccac aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt
    28741 ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt
    28801 tacttttctt taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata
    28861 agtcgctata cttcggattt ttgccatgct atttctttac atctctaaaa caaaacataa
    28921 cgaaacgcac tgccggacag acaaatgaac ttatccctac gacgctctac cagcgccctt
    28981 cttgcctcgt cgttgttatt aaccatcgga cgcggcgcta cgctgccatt tatgaccatt
    29041 tacttgagtc gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg
    29101 ctcactattg gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag
    29161 aaacgctata tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta
    29221 gtgaataacg tgacgctggt ggtgctcttt tttgccctta ttaactgcgc ctattccgtt
    29281 tttgctaccg tgttgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa
    29341 atcttctcaa tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc
    29401 acgctgttgg tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg
    29461 tttcccatgc ttttcattca aatttgggta aagcgcagcg agaaaatcat cgccacggaa
    29521 acaggcagtg tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc
    29581 tgctctggtt ttctggcttc ctttgttagc ggcgcatttg cttcatgcat ttcacaatat
    29641 gtgatggtga ttgctgatgg agattttgcc gaaaaggtgg tcgcggttgt tcttccggtt
    29701 aatgctgcca tggtagttac gttgcaatat tccgtgggcc gccgccttaa cccggttaac
    29761 atccgcgcgc tgatgacagc aggcaccctc tgtttcgtca tcggtctggt tggttttatt
    29821 ttttccggca acagcctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa
    29881 atcatttatg cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa
    29941 gccagctatt tttctgccca gtctttaggc tggcttggtg ctgcaattaa cccattagtg
    30001 agtggcgtag tgctaaccag cctgccgcct tcctcgctgt ttatcatctt agcgttggtg
    30061 atcattgctg cgtgggtgct gatgttaaaa gggattcgtg caaaaccgtg ggggcagccc
    30121 gcgctttgtt gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttac
    30181 ctctgctaat atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac
    30241 cataaacgga ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata
    30301 attttatttt ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg
    30361 gagataatcc ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt
    30421 atgacggcga aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt
    30481 gttttgcttt tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag
    30541 catcagtttt ctcaatgaat gttaaacgga gcttaaactc ggttaatcac attttgttcg
    30601 tcaataaaca tgcagcgatt tcttccggtt tgcttaccct catacattgc ccggtctgct
    30661 cttccaatga ccacatccag aggctcttca ggaaatgcgc gactcacacc tgctgtcacg
    30721 gtaatgttga tatgcccttc agaatgtgtg atggcatggt tatcgactaa ctggcaaatt
    30781 ctgacacctg cacgacatgc ttcttcatca ttagccgctt tgacaataat gataaattct
    30841 tcgcccccgt agcgataaa
//
LOCUS       DAZFDP010000040        30619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFDP010000040 DAZFDP010000000
VERSION     DAZFDP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHJ1G_001943"
     CDS             complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHJ1G_001943"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO4763803.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            679..2829
                     /gene="dinG"
                     /locus_tag="ACHJ1G_001944"
     CDS             679..2829
                     /gene="dinG"
                     /locus_tag="ACHJ1G_001944"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4763804.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHJ1G_001945"
     CDS             2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHJ1G_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4763805.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHJ1G_001946"
     CDS             3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHJ1G_001946"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4763806.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHJ1G_001947"
     CDS             5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHJ1G_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4763807.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHJ1G_001948"
     CDS             complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHJ1G_001948"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4763808.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            6869..7540
                     /gene="cecR"
                     /locus_tag="ACHJ1G_001949"
     CDS             6869..7540
                     /gene="cecR"
                     /locus_tag="ACHJ1G_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4763809.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHJ1G_001950"
     CDS             7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHJ1G_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4763810.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHJ1G_001951"
     CDS             8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHJ1G_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4763811.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHJ1G_001952"
     CDS             10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHJ1G_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4763812.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHJ1G_001953"
     CDS             11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHJ1G_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4763813.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHJ1G_001954"
     CDS             complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHJ1G_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4763814.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHJ1G_001955"
     CDS             13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHJ1G_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4763815.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13773..15014
                     /gene="clsB"
                     /locus_tag="ACHJ1G_001956"
     CDS             13773..15014
                     /gene="clsB"
                     /locus_tag="ACHJ1G_001956"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4763816.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHJ1G_001957"
     CDS             15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHJ1G_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4763817.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16006..16719)
                     /gene="ybhM"
                     /locus_tag="ACHJ1G_001958"
     CDS             complement(16006..16719)
                     /gene="ybhM"
                     /locus_tag="ACHJ1G_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4763818.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(16924..17628)
                     /gene="ybhL"
                     /locus_tag="ACHJ1G_001959"
     CDS             complement(16924..17628)
                     /gene="ybhL"
                     /locus_tag="ACHJ1G_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4763819.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(17765..18217)
                     /gene="moaE"
                     /locus_tag="ACHJ1G_001960"
     CDS             complement(17765..18217)
                     /gene="moaE"
                     /locus_tag="ACHJ1G_001960"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4763820.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(18219..18464)
                     /gene="moaD"
                     /locus_tag="ACHJ1G_001961"
     CDS             complement(18219..18464)
                     /gene="moaD"
                     /locus_tag="ACHJ1G_001961"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4763821.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(18457..18942)
                     /gene="moaC"
                     /locus_tag="ACHJ1G_001962"
     CDS             complement(18457..18942)
                     /gene="moaC"
                     /locus_tag="ACHJ1G_001962"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4763822.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(18945..19457)
                     /gene="moaB"
                     /locus_tag="ACHJ1G_001963"
     CDS             complement(18945..19457)
                     /gene="moaB"
                     /locus_tag="ACHJ1G_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4763823.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(19479..20468)
                     /gene="moaA"
                     /locus_tag="ACHJ1G_001964"
     CDS             complement(19479..20468)
                     /gene="moaA"
                     /locus_tag="ACHJ1G_001964"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4763824.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(20456..20600)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            20865..21773
                     /gene="yvcK"
                     /locus_tag="ACHJ1G_001965"
     CDS             20865..21773
                     /gene="yvcK"
                     /locus_tag="ACHJ1G_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4763825.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(21965..23986)
                     /gene="uvrB"
                     /locus_tag="ACHJ1G_001966"
     CDS             complement(21965..23986)
                     /gene="uvrB"
                     /locus_tag="ACHJ1G_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4763826.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(24565..25242)
                     /gene="bioD"
                     /locus_tag="ACHJ1G_001967"
     CDS             complement(24565..25242)
                     /gene="bioD"
                     /locus_tag="ACHJ1G_001967"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4763827.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(25235..25990)
                     /gene="bioC"
                     /locus_tag="ACHJ1G_001968"
     CDS             complement(25235..25990)
                     /gene="bioC"
                     /locus_tag="ACHJ1G_001968"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4763828.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(25977..27131)
                     /gene="bioF"
                     /locus_tag="ACHJ1G_001969"
     CDS             complement(25977..27131)
                     /gene="bioF"
                     /locus_tag="ACHJ1G_001969"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4763829.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(27128..28168)
                     /gene="bioB"
                     /locus_tag="ACHJ1G_001970"
     CDS             complement(27128..28168)
                     /gene="bioB"
                     /locus_tag="ACHJ1G_001970"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4763830.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28255..29544
                     /gene="bioA"
                     /locus_tag="ACHJ1G_001971"
     CDS             28255..29544
                     /gene="bioA"
                     /locus_tag="ACHJ1G_001971"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4763831.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            29603..30079
                     /gene="ybhB"
                     /locus_tag="ACHJ1G_001972"
     CDS             29603..30079
                     /gene="ybhB"
                     /locus_tag="ACHJ1G_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4763832.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         7177 a         8106 c         8099 g         7237 t
ORIGIN      
        1 acaacaagca cttgtcagca atttgagaac acgggaaaaa atttatctgt tttaccgccc
       61 ataagccacc gagacgttac ttctctatag ccaattgttc gcgcaactcc attaaaaggt
      121 agcccagtcg attcttgccc ttaccattac caccgtctcc ccagtaagca tcgttttccg
      181 tatgctcaac cagttttgcg ggcgcggttg ccagcaagag cgcacgcagt tctggatgct
      241 gttcgaattt agcgcgaagt gcttttcgca tcacctgttc tttgaccgac tcccagtttt
      301 tacgcagagg cttagaacga tcgcgcccca tgcgtgcggc gaccataggg gaagaaaccc
      361 gacggatctc ttcgcggtat ttttcatcaa ggaatttttg tgcctgaaaa tagtgttctg
      421 aggtgggcca ggttttcccg tcaaccttga tgggccaggc ggcaaagtta gaaaaatcac
      481 cgtagtcatc gctggtgctg tagaaattta tgatggtgtc ttgcatgacg tgttggatcc
      541 tttgtgctcg aacgggcatt aaaccgcatt atgttggtgg ttattgcgag ccgctttcca
      601 gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc acaatattgg ctgtttatac
      661 agtatttcag gttttctcat ggcattaacc gccgcgctta aagcgcaaat tgccgcctgg
      721 tataaggcgc ttcaggaaca gatccccgac tttattcccc gtgcgccgca gcggcagatg
      781 attgcggacg tcgccaaaac gctggccgga gaagaagggc gacatctggc gattgaagcc
      841 cccaccggcg ttgggaaaac gctctcctat ttgatccccg gcatcgccat tgcccgcgaa
      901 gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat tgcaggatca gatttacagc
      961 aaagatttac cgctgctgaa aaagatcatt cccgatctta aattcactgc cgcttttggg
     1021 cgtgggcgct acgtttgccc gcgtaatctg acggcgctcg ccagcactga gcccacgcaa
     1081 caggatctgc tggcgtttct tgacgacgaa ctgacgccaa ataatcagga agagcaaaaa
     1141 cgttgtgcga agctgaaggg cgatctcgac acttataaat gggatggtct gcgcgatcat
     1201 actgatatcg ctatagatga cgatctctgg cgtcgtttaa gtaccgacaa agccagctgc
     1261 ctcaaccgca actgttacta ctatcgtgaa tgcccgtttt ttgttgctcg tcgggagatt
     1321 caggaagcgg aagtggtggt ggcaaaccat gcgctggtga tggcggcgat ggaaagcgaa
     1381 gccgtactac ctgacccgaa aaatctgctg ctggtgctgg acgaaggtca tcacctgcca
     1441 gatgtggccc gggatgcgct ggagatgagt gccgaaatca ccgcgccctg gtatcgacta
     1501 cagctggact tgttcacgaa actggtcgct acctgcatgg agcagtttcg cccgaagacc
     1561 atcccaccgc tggcgatccc tgaacgtttg aatgcgcatt gtgaagagtt gtatgagctt
     1621 atcgcctcgt taaacaacat tctcaatctc tacatgcctg ccgggcagga ggcagagcac
     1681 cgttttgcga tgggcgaact gcctgatgaa gtgctggaga tctgccagcg gctggcaaaa
     1741 ctcaccgaga tgctgcgtgg tctggcggag ttatttctta acgatttaag tgagaaaacc
     1801 ggcagccacg atattgtgcg tctgcatcgg ttgattttgc agatgaaccg cgcgttgggg
     1861 atgttcgagg cgcaaagcaa actctggcgg ctggcgtctc tggcgcaatc ttccggtgcg
     1921 ccggtgacca aatgggcgac gcgggaagag cgcgaagggc agctgcatct ctggtttcac
     1981 tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc tgtggcgcag tattccgcac
     2041 attattgtca cctccgcaac cttgcgttcg ctgaacagtt tttcgcgttt gcaggagatg
     2101 agtggtctga aagagaaagc gggcgaccgt tttgtggcgc tggattcccc ctttaaccac
     2161 tgcgaacagg gcaaaattgt tattccccgg atgcgctttg agccttccat cgacaacgaa
     2221 gagcagcata ttgccgaaat ggcggccttt ttccgtaagc aggtggagag caaaaaacat
     2281 ctcggtatgt tggtactgtt tgccagcgga cgggcgatgc agcgctttct cgactatgtg
     2341 acggatttac gcctgatgct gctggtgcag ggcgatcagc cgcgttatcg cttagttgaa
     2401 ctgcaccgca aacgcgttgc caacggtgag cgtagtgtgc tggtgggctt acagtcattt
     2461 gccgaagggc ttgatttgaa aggtgatctg ctcagccagg tgcatatcca caaaatcgct
     2521 tttccgccca tcgacagccc ggtggtgatc accgaagggg aatggctgaa aagcctcaac
     2581 cgctatccgt ttgaggtgca aagcctgccg agcgcctcgt ttaacctgat tcagcaggtt
     2641 gggcgactga ttcgaagcca cggttgctgg ggcgaagtgg ttatctacga caaacgcttg
     2701 ctgaccaaaa attatggcaa gcgactactg gatgcattac cggtatttcc gatagagcaa
     2761 ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa cgaaatcccc acgccgtcgg
     2821 cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg actatcgcaa aatcattaaa
     2881 gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc gggatactgc gcgcggtctg
     2941 tatgcccata tgctcaatgg tgaagtccct gaccttgagt tgggcggcgt attgattgcg
     3001 ctgcgtatca aaggggaagg ggaagcagag atgctcggct tttacgaagc catgcaaaat
     3061 cacaccatca agctgacgcc gcctgcaggc aagccgatgc cgattgtcat ccccagttac
     3121 aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg cgattctcct gcacaaactc
     3181 ggttttcctg tggtggttca cggggttagc gaagatccaa cccgcgtgct gactgaaacc
     3241 atttttgaat tgatgggtat tacgccaacg ttgcacggcg gacaggcgca ggcgaagctc
     3301 gacgaacatc aaccggtgtt tatgccagtc ggcgcgtttt gcccgccgct ggaaaaacaa
     3361 ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac ataccctggc gaaactggca
     3421 acgccatttg ccgaaggtga ggcgctgcgt ctttccagcg tttcgcatcc ggaatacatt
     3481 ggacgcgtcg cgaagttctt tagcgatatc ggcgggcggg cgctactaat gcatggtact
     3541 gaaggtgaag tgtatgctaa tccgcagcgc tgcccgcaga tcaatctcat tgaccgtgaa
     3601 gggatgcggg tgctgtatga aaaacaggac actgctggta gcgagttact gccacaagca
     3661 aaagatccgg aaaccacggc gcagtggatt gagcgttgcc ttgctggcag cgaaccgatt
     3721 cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg ctacgggtga agcggcaact
     3781 atcagcgacg gcctggcgcg cgttaatcag gcattttaat tcttttctca gccggatgag
     3841 ccatgtcatc cggcttttac cccaccgcaa tatgaaattc ctgcatcttt attgaccttc
     3901 ccacgcccgg cgtgcagcat aaaaatacaa caaacacata acataaacag gagttaacca
     3961 tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag ttttattcag gctgtatttc
     4021 gtcagatggg tagcgaggaa caagaagcga aattagttgc cgatcattta atcgcggcaa
     4081 acctggcagg gcatgattca catggtattg gcatgatccc aagctatgta cgctcctgga
     4141 gtcaggggca cctgcaaatt aaccatcatg ccaaaaccgt taaagagtcg ggggcggcgg
     4201 tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc acatgaagcg atggcgctgg
     4261 ggattgagaa agcgcataag cacggtattg ccgccgtggc gctacataac tcgcatcata
     4321 tcggccgtat cggttactgg gcggagcagt gtgcagcggc ggggtttgtc tctatccact
     4381 ttgttagcgt ggtcggtatt ccaatggtcg cgccgttcca cggtcgcgac agccgctttg
     4441 gcaccaatcc gttctgtgtg gttttccctc gtaaagataa tttcccgctg ttgcttgatt
     4501 acgccaccag cgccattgca tttggtaaaa ctcgcgtcgc ctggcataaa ggcgtccccg
     4561 tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac gaccaatccg gcggtaatgc
     4621 aggagtcgcc gttgggggcg ctgttgacct ttgccgaaca taaaggctac gcccttgcag
     4681 cgatgtgtga aattcttggc ggggcgcttt ctggcggtaa aacgacgcat caggaaacgt
     4741 tacaaaccag tcccgatgcc attcttaact gcatgaccac tatcatcatc aacccggaac
     4801 tcttcggcgc gccggattgt aacgcgcaga ccgaagcctt tgccgagtgg gtgaaagcct
     4861 cgccgcatga tgatgataag ccgattttgc taccgggcga gtgggaagtg aacacgcgtc
     4921 gcgaacggca gaagcagggg attccactgg atgcgggaag ctggcaggcc atttgtgatg
     4981 cagcgcggca gattggtatg ccggaagaga cgttgcaggc tttctgtcag cagttagcca
     5041 gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag ggtaacatag gatcaatgag
     5101 ggttagagca tatgcgtctg tcggcaaaca gacagggaaa tacttgtgct ggacgtagcg
     5161 taaacgcctg atccgtccaa cggttcggat tttgtagata ctgataagcg tagcgcatca
     5221 ggcagttttg cgtttgttca tcagcctgat gccgggcata gcccggcata aaagattact
     5281 gcgcagcggt aggtttacgc ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat
     5341 cgccgaggcg gcgagacggt ttttctgcag gtttcgcgct tgcagatttt gcgccacctt
     5401 ccccacggcg tggttgctgt tgaccgcgac caccaccttg cccacgaccg ccgtcgccac
     5461 gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc tcatagcccg
     5521 gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc agcagtttgt
     5581 gttcatcaac acacaccagc gacaacgctt caccggtagc agccgcacga ccggtacgcc
     5641 cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg acgtgcggca
     5701 actcttcgat atccagaccg cgcgcagcga tatcggtcgc caccagtacg cgaatgtcac
     5761 ccgatttaaa atcagccagc gcacgggtac gcgcaccttg cgatttattc ccgtggatcg
     5821 ccgcactacg gatgccatct ttattgagct gctcagccag atggttagcg ccgtgtttgg
     5881 tacgggtaaa caccagcacc tgctgccagt tccctttacc aatcatgtgc gacagcaatt
     5941 cgcgtttgcg tttcttatca acaaagtgaa cgtgctgggt cacctgatca gacgcggtat
     6001 tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag tttttccgcc agggctttaa
     6061 tatcgtcaga gaaggtcgca gagaacagca ggttctggcg cttcgctggc agttttgtta
     6121 acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct tcatcgagga
     6181 cgaggatttc aacctgatcc agcttcactg cattttgatg ttccaggtcc agcaaacgtc
     6241 ccggtgttgc caccagaaca tcaacgccgc cgcgcagctt catcatttgc gggttaatgc
     6301 tgacaccgcc aaacaccacc agcgaacgaa tgttcaaata tttgctgtaa tcacggacgt
     6361 tttcgccaat ctgcgcagcc agttcacggg tcggggtaag aatgagcgca cgtaccggac
     6421 gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc agcgtaaagc
     6481 ccgctgtttt accggtaccg gtctgggcgc tagccatcag gtcgcggcct tccagcaccg
     6541 cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg gcaacggcgc
     6601 gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg aaccgccccg
     6661 accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa aaccacaatg
     6721 tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat ccctgtgact
     6781 ggcgtagcta aaattcttag ccatactgga caactcccat atggagtcat aatcttaatc
     6841 aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa tcaagggtga
     6901 acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat atggaatgaa
     6961 cgccaccacc cgcgagatag ccgcccaggc cgggcagaat atcgctgcca tcacctacta
     7021 cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg attttattgg
     7081 cgagcagttc cgtccgcacg ccgaagaggc cgaacgcttg ttcgcacaac cacagcctga
     7141 tcgcgcagcc atccgtgaac tgatccttcg cgcctgcagg aacatgatta agctgctcac
     7201 ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct ctcccacggc
     7261 agcctatcac ctggtgcacg aacaggtgat tagcccgcta cacagccacc tgacacgcct
     7321 gattgccgcc tggaccggct gcgacgccaa cgatacccgc atgatcctcc atactcatgc
     7381 gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt tacgtaccgg
     7441 ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga cctgtcatat
     7501 cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg
     7561 atcggactgg cggtagtggt acttgccgcc gtggttgccg gaggctactg gtggtatcaa
     7621 agccgccagg ataacggcct gacgctgtat ggcaacgtgg atattcgtac ggtgaatctt
     7681 agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga tgctatcaaa
     7741 gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct gatgcaggcg
     7801 aaagcgggtg tttcggtggc acaggcgcag tatgacctga tgcttgccgg gtatcgcgat
     7861 gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc aagccgccta tgactatgcg
     7921 cagaacttct ataaccgcca gcaagggttg tggaaaagcc gcaccatttc ggcaaacgac
     7981 ctggaaaatg cccgctcctc gcgcgaccag gcgcaggcaa cgctgaaatc agcacaggat
     8041 aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgcccaggc gaaagccagc
     8101 ctcgaacagg cgcaggcgca actggcgcag gcggagttga atttgcagga ctcaacgttg
     8161 atagccccgt ctgatggcac gctgttaacg cgcgcggtgg agccaggcac ggtcctcaat
     8221 gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg cgcttatgtt
     8281 gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta taccgatggt
     8341 cgcccggaca agccgtatca cgggcagatt ggtttcgttt cgccgactgc tgaatttacc
     8401 ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct gcgtattgtg
     8461 gtgaccgacg ccgatgatgc gttacgccag ggaatgccgg tgacggtgca attcggtaac
     8521 gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac gctttccggg
     8581 catggacaag cccgccgtcg cgccgctcga ttgtaccatt cacgccggtt atgtgacggg
     8641 gctggtgggg ccggatggtg caggtaaaac cacgctaatg cggatgctgg cgggattact
     8701 gaaacccgac agcggcaatg ccacggtgat tggctttgat ccaatcaaaa acgacggcgc
     8761 gctgcacgcc gtgctcggct atatgccgca gaaatttggt ctgtatgaag atctcacggt
     8821 gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg cacgtaagca
     8881 aacttttgct cgcctgctgg agtttacgtc tcttgggccg tttaccggac gcctggcggg
     8941 caagctctcc ggtgggatga aacaaaaact cggtctggcc tgtaccctgg tgggcgaacc
     9001 gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac ggcgcgaact
     9061 gtggcagatg gtgcatgagc tggcaggcga agggatgtta atcctctgga gtacctcgta
     9121 tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aacgaaggcg agttgctgta
     9181 tcagggggaa ccaaaagccc tgacacaaac catggccgga cgcagttttc tgatgaccag
     9241 tccacacgag ggcaaccgca aactattgca acgcgccttg aaactgccac aggtcagcga
     9301 cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaggcca caccagacga
     9361 tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc cgcgttttga
     9421 agatgcattt attgatttgc tgggtggtgc cggaacctca gaatcgccgc tgggcgcaat
     9481 attacatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag aactgaccaa
     9541 gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac gtggggagat
     9601 ttttggtttg ctggggccaa acggcgcggg taaatcgacc acctttaaga tgatgtgcgg
     9661 tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga aagagagttc
     9721 tggtaaagcg cgccagcatc tcggctatat ggcgcaaaag ttttcgctct acggtaacct
     9781 gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca
     9841 gaacgaaaaa atctcccgta tgagcgaggc gttcggcctg aaaagtatcg cctcccacgc
     9901 caccgatgaa ctgccattag gttttaaaca gcggctggca ctggcctgct cgctgatgca
     9961 tgaaccggac attctgtttc tcgacgaacc gacgtccggc gttgatccga ttactcgccg
    10021 cgaattctgg ctacatatca acagcatggt agagaaaggc gtcacggtga tggtcaccac
    10081 ccactttatg gatgaagcag aatattgcga ccgcatcggc ctggtgtacc gtgggaaatt
    10141 aattgccagc ggcacgccgg atgatttgaa agcgcagtcg gccaacgacg agcaacccga
    10201 tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg agcatagcaa
    10261 tgagtaaccc gatcctgtcc tggcgtcgcg tacgggcgct gtgcgttaaa gagacgcggc
    10321 agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg ctactgctgt
    10381 ttatttttgg ttacggcatt aacctcgact ccagcaagct gcgggtcggg attttactgg
    10441 aacagcgtag cgaagcggcg ctggatttca cccacaccat gaccggttcg ccctacatcg
    10501 acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc
    10561 gcggtctggt ggttattccg gtggattttg cggaacagat ggagcgcgcc aacgccaccg
    10621 caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt gtacaggggt
    10681 atgtcgaagg gatctggcag atctggcaaa tgcagcgagc ggaggacaac gggcagactt
    10741 ttgaaccgct tattgatgta caaacccgct actggtttaa cccggcggcg attagccagc
    10801 acttcattat ccccggtgcg gtgaccatta tcatgacggt catcggcgcg attctcacct
    10861 cgctggtggt ggcgcgagaa tgggaacgcg gcaccatgga ggctctgctc tctacggaga
    10921 ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc gggatgctgg
    10981 cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg cgtgccgtat cgcgggtcgc
    11041 tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg atggggctgc
    11101 tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg aacgccgctt
    11161 ttctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg cccgcggtga
    11221 tccgcgcggt gacgtacatt attcccgctc gttatttcgt cagcaccctg caaagcctgt
    11281 tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt gctgtttttg atcgcttcgg
    11341 cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat tagggagaag
    11401 agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgct gctgcgcgaa
    11461 ccgcaaaccc gcgcgattct gattttacct gtgcttattc aggtgattct gttcccgttc
    11521 gccgccacgc tggaagtgac taacgccacc atcgccatct acgatgaaga taacggcgag
    11581 cattcggtgg agctgaccca acgttttgcc cgcgccagcg cctttactca tgtgctgctg
    11641 ctgaaaagcc cacaggagat ccgcccaacc atcgacacac aaaaggcgtt actactggtg
    11701 cgtttcccgg ctgacttctc gcgcaaactg gataccttcc agaccgcgcc gttgcagttg
    11761 atcctcgacg ggcgtaactc caacagtgcg caaattgccg ccaactacct gcaacagatc
    11821 gtcaaaaatt atcagcagga gctgctggaa ggaaaaccga aacctaacaa cagcgagctg
    11881 gtggtacgca actggtataa cccgaatctt gactacaaat ggtttgtggt gccgtcgctg
    11941 atcgccatga tcaccactat cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa
    12001 cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg gcagatcttc
    12061 atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat tgtgctggcg
    12121 attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt ctactttacg
    12181 atggtgattt acggtttatc gctggtggga ttcggtctgt tgatttcatc actctgctcg
    12241 acacaacagc aggcgtttat cggcgtgttt gtttttatga tgcccgccat tctcctttcc
    12301 ggttacgttt ctccggtgga aaacatgcca gtatggctgc aaaacctgac gtggattaac
    12361 cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc gagtctggat
    12421 attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg gtcagcggcg
    12481 tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca aagacactac
    12541 cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt tttccagtac
    12601 acgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg tggtcagctc
    12661 ttccagcacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa acaccaacat
    12721 catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa ccagagtctt
    12781 actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa tctgccagca
    12841 acttagacca gttgcgacac gaacacgttg ttgccacttc atggcttctc tcctgaagaa
    12901 tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt
    12961 ttgtgacgcc gactacacta tttcaatgag ataagaggca aagacaggaa aattatgccc
    13021 gatcaaacac aacaattttc gttcaaggtg ctcaccatca acattcacaa aggttttacc
    13081 gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg cggtgcgtac cgtcagcgcc
    13141 gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa
    13201 aactggcccg atacctcgca ctacgagttt ctcgccgaca cgatgtggag cgattttgcc
    13261 tacggtcgca atgccgtata cccgaaaggg catcacggca acgccgtact gtcgcgttat
    13321 cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg
    13381 ctctactgcc gcattgtgcc accgatgacc ggaaaagcga ttcatgtgat gtgcgtacat
    13441 ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tgctcgccga atgggtgaat
    13501 gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg gcggcaaaaa
    13561 gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg cgcccacgga
    13621 cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag gatctacgtc
    13681 aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg acacctttct
    13741 gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc gaaggcaata
    13801 agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag gcgattggcg
    13861 aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggatgac gtcggcaaac
    13921 agctgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa gtcttgctgg
    13981 atggctacgg ttcgccggat ctcagcgatg agtttgtcaa tgaactgacg gcagctggcg
    14041 tggtatttcg ctactacgat ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc
    14101 gccggatgca tcgcaaaatt gtggtgatcg acgcgcgtat cgcctttatt ggcgggctga
    14161 attactccgc cgagcatatg tccagctacg gtccagaggc taaacaggat tacgcggtac
    14221 gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac ctgcctggac
    14281 agagcgcggc acgacgctgg tggcgacgtc atcacaaagc ggaagagaac cgccagccgg
    14341 gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat gatattgaac
    14401 gtcactatct gaaaatgctc actcaggcgc ggcgagaagt cattatcgcc aacgcctact
    14461 tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc ggggtgcgga
    14521 tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt gcgcgcttgc
    14581 tgtataacta tctggttaaa ggcggcgttc aggtatttga gtaccgccgc cgcccgctcc
    14641 acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt aatctcgatc
    14701 cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt cattttaacc
    14761 agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag gtcgatgaga
    14821 ccatgctgcc gaaacgcacc tggtggaacc tgaccaaaag cgtgctggcg ttccactttt
    14881 tacgccactt cccggcgctg gttggctggc ttccggcaca cacaccacgt ctggcgcagg
    14941 ttgatccgcc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg
    15001 gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc ttagcaaaga agatcctcac
    15061 ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa aagtggactg
    15121 ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta gtgcggtcgg
    15181 gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc gcttttactg
    15241 cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct acgccttcaa
    15301 cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt actcccgact
    15361 agggttaccg ggcagcacta ttacgcggat tttctcgctc agtattacca ccaactggct
    15421 gggctatatt ttactggcag ggattatctt taccgcaggc gtggtggagt tgccggatca
    15481 ctggtatgtc gatcaaacta cgctgcgtat tctcggcatt ggcttactga tgattatcgc
    15541 ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa
    15601 gctggtgctg ccttcatgga aattcgccct cgctcagatg ctgatttcca gcgttaactg
    15661 gatggtaatg ggggcgatta tctggctgtt acttggtcaa agcgtgaact atttctttgt
    15721 actgggcgtg ttactggtta gtagtattgc tggcgtcatc gtgcatattc cggtggggat
    15781 cggtgtgctg gaagcggtgt ttatcgcact actggctggg gagcatacct cgaaagggag
    15841 cattatcgcc gccctgctcg cttatcgcgt cctgtattac tttatcccgc tgctgctggc
    15901 gctgatctgt tacctgctac tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc
    15961 ggcgatgtga agtttagtta agttctttag tatctgcatt tacgactaat gaaaaaaacg
    16021 cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa agaagccgat
    16081 aaaatagtaa tagagtgtta atgcacctgt aacagcgcaa cgaaccggac tccacaactc
    16141 ttcactatgg catttccccg ccaattcaag gagcttattt cgtccatgcg agataatgcc
    16201 tgaccataac acaatcatta agcaacttat tacccaaacg ggtcgttcac tcattaatgc
    16261 ggcattcatg atgatcacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg
    16321 agaaccagga tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc
    16381 cgcagtgctg aacacgacga taatggcagg tacaggagct aaacttgcaa cgacaatcgc
    16441 caccaaagag agtaaaaaca gatatattac gcctgcgatg acggcattta tcttgttgat
    16501 atccttactt aagccataca tacaaactaa taataaccct atggcgacat acattgttgg
    16561 tgaatcatat ttggttgccg taatttcgag atacttagct ccccaggcca gtcctgcggt
    16621 tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg tctcgtgctg
    16681 aaaatctaat ttattgctgt tttgtgagta tgattccatt cttttagttg tccttagaaa
    16741 aaatattaat attcaccatc aataaattta aaaccacgca tacaaaatac ttttgtattt
    16801 atggatacgg aaaacgcttc cgctcgccat aaggcgtatt ttatacttgg tacttgttaa
    16861 gtgtcgtttt tagtaagaaa aaaaatcccg acacatggcc gggatattga tggtgaaaaa
    16921 gaattaacgg cggttgccaa aaattcgcag caacatcagg aacaggttga tgaagtccag
    16981 atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc
    17041 aatctgctca cccatatttt tcaatttctg agtgtcatac gccgtcaatc cgacaaagac
    17101 aatcacgccg atgtaggtaa ctgcccacat caatgcttcg cttttcagcc agaagttgac
    17161 cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa agccacttaa
    17221 atcgcgcttc gtggtgtaac cgtacaggct cattgcgccg aacatcccgg cagtaacgac
    17281 gaaagtactg gcgatagaag cagcggtata gacaatgaat atactggaaa gcgtaagacc
    17341 cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca gcttttgaat
    17401 catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac
    17461 acggttagta aacaacagct ccatcacggc cgcggaatta gccgcatacc aggcaacaaa
    17521 tgcggtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat aagtttgcaa
    17581 gccagcccgg ggttgtacga ttgaatcaga acgtgggaat ctgtccatga cgatctcctg
    17641 aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat cgacgcagcg
    17701 taaacgcatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa
    17761 aaaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcaacc cagcggtcgc
    17821 cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
    17881 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccgaca aaaacgattt
    17941 catcgcccgg ccataattcc ccgatgcggt gaatcacagt gacgcgcccc agcggccagc
    18001 ggttacgcgc ttcatcaaca atttctgcca gtgctttttc agtcatcccc ggatagtgtt
    18061 cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccagtaaagg
    18121 tgactaccgc accgtcttcg tcacgctccg ccagccacgg gtactcttct cctacgctga
    18181 acggctgcgg accaacaaca attttggttt ctgccatctt aacctccggt taccggcggg
    18241 aagaaagcta cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag cgtctggttg
    18301 acggcagcca gtaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc
    18361 atgtgctggc gtaacgcttc aacagttggg aaatccgcag ccacttcggt tgcatctgtt
    18421 cccaccaact cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa
    18481 gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatca ccatatcttt
    18541 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaatgc
    18601 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gcacccgatt
    18661 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg gatgacagag
    18721 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa
    18781 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
    18841 gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat
    18901 gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
    18961 ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcgatgat attttcccat
    19021 gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttggcgacg
    19081 cccgctaccg cacgagattg caacgtggaa gtgccaatct cttcaaacga caacatacgg
    19141 aacacttcac caaaaccttc aatttcacgg tcgaacaacg gcagcaatgc ttcgggagcc
    19201 tgatcacctt ccgtcaggcc agtaccaccc gtaatcaata ccacttgtac atcgtcgctg
    19261 gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
    19321 tcgacaacgt gatggcccgc ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg
    19381 tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
    19441 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag
    19501 ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc
    19561 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc
    19621 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
    19681 aggcggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg
    19741 gcgtaatctg gatggcaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
    19801 atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
    19861 aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatagggcga
    19921 tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat taacatcacg catcagcacg
    19981 gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcaatccc cgccatgacc
    20041 tggttgaatt tatcctgccc ggtaatagcg tgaaactggc gggcgtccag actgtcgaca
    20101 ctgacgttaa tgccagtaag tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg
    20161 taaccattgg tggtgaccgc aatctggcgg atagcgtcgt tttcccgcac agcggcgatg
    20221 atatcggtaa agtcgcggcg taaagacggc tcaccgcctg tcaggcgcac tttttcggtg
    20281 cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtaag aaagccttta
    20341 ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac
    20401 acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
    20461 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
    20521 gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca ggtgcagagg
    20581 ctagagtgtt tagtggttat gccgatacta gcgtgtaaat gagattttta ccatccacac
    20641 tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
    20701 aaaatggcgg ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta
    20761 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca
    20821 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
    20881 tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
    20941 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacc gataatggtg
    21001 gctcgacggg gcgtattcgc cgttcagaag gcggcattgc ctggggcgat atgcgcaact
    21061 gcctcaacca gctgataacg gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg
    21121 gtggcaatgg cgaactttcc ggtcataatc tcggaaactt gatgttaaag gcgctggatc
    21181 accttagcgt gcggcctctg gaagccatca atttaattcg taatctgctg aaagtggata
    21241 cgcatttgat tccaatgtca gagcatcctg ttgatctgat ggcgattgac gatcaggggc
    21301 atgaagttta cggcgaggtc aatatcgacc agttaactac gccgattcaa gagttattgt
    21361 taacgcctaa tgtacccgca acgcgtgagg cggttcacgc tatcaatgaa gcggatctca
    21421 tcattattgg gcctggcagt ttttatacca gcctgatgcc aattctgctg ctgaaggaaa
    21481 tcgcccaggc attacgccgc acgccagcgc cgatggttta tatcggcaat ctggggcgtg
    21541 agttgagttt acctgcggct aatttgaagc tggaaagcaa gctggcaatt atggagcagt
    21601 atgttggtaa aaaggtcatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg
    21661 tgaaagagcg gattgtgatc caggaggtac tggaggccag cgatattccg tatcgtcatg
    21721 accgccagtt gttacataac gcgctggaaa aggcgttaca ggctttaggt taacggaaat
    21781 ccgaaggaaa attccggctt cctattgaag acaaagtgcg cgttgtttat gccggatgcg
    21841 gcgtgaacgc cttatccggc ctacaaaccg cgcaaattca atatattgcg gagaaaatgt
    21901 aggcctgata agcgtagcgc atcaggctgt tttccgtttg tcatcagtct tcttcgctat
    21961 cctgttacga tgccgcgata aacagctcac gcagctgatg caactggtca cgaatttgcg
    22021 ccgcttcttc gaactccaga ttctgcgcgt gttgcatcat caacccttcc agctcatgga
    22081 ttttctgctg caacgcttta ggcgacatat ccatcggcac attatccggc tcaacaatcg
    22141 gtcgcgattt tcctctgccc ttcgctttgg ttttagcaat gttctgcccc aacgccagga
    22201 tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct
    22261 gctgtttctc gcggcgacgt tcggtttcgc caatcgcttt cgccattgat ggggtgatct
    22321 tatcgccgta gagaatcgct ttaccgttaa cgttacgtgc cgcacgacca atggtctgga
    22381 tcaacgaacg ttcggaacgc aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg
    22441 aaacttccgg catatccaga ccttcacgca gtaagttgat ccctaccaat acgtcgaact
    22501 cacccagacg caagtcgcgg ataatctcca tacgctcgac ggtgtcgata tctgagtgaa
    22561 gataacgcac gcgctcaccg tgttcttcga gatattcggt aagatcttcc gccattcgct
    22621 tggtcagtgt ggtgaccagt acgcgttcgt taattgccgc tcgctgacga atctccgaaa
    22681 gaagatcatc aacctgtgtc gccaccggcc gcacttcgat aatcgggtca agcaatccgg
    22741 ttggacgcac cacctgatcc accacatcgc cgccggattt ttccagctcg taattacccg
    22801 gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca aacttaagcg
    22861 gacggttatc cagcgctgat ggcaggcgga agccgtactc caccagtgtc tctttacgcg
    22921 cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacca
    22981 ccagcagccc atcagcaggg aggtaatcaa acagcgtcgg cggtggctca cccggtccac
    23041 gaccggagag gaagcgcgag tagttttcaa tccccgaaca gtagcccagc tcgttcatca
    23101 tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt
    23161 ccaacagcac tttgcgtctg gcggccagct cttctttgat ctcctccatc gcctgtacga
    23221 tgcgctcgcg cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg
    23281 aaacaatctg cccggtcagc gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca
    23341 gttccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatctatc acctcgccac
    23401 gaacgcggaa agtaccacgc tggaatgctt gatcattacg agcgtattgc agctccgcca
    23461 gtcggcgcag aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct
    23521 tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagacgcc accacaacca
    23581 catcacgccg ctccagcatc gctttggtgg cggacaaacg catctgctca atatgttcgt
    23641 taaccgaggc atctttctca atgaaagtgt cggaactcgg tacataggct tccggctgat
    23701 agtagtcgta gtaggaaacg aaatattcca ccgcgttttc cgggaagaac tctttcattt
    23761 cgccatacag ctgggccgcc agcgttttgt tgggcgcaag taccatggtt gggcgctgaa
    23821 ggtcagcaat gacattggca atggtgaatg ttttcccgga gccagtcacg ccaagtaacg
    23881 tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat
    23941 cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga
    24001 aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc caacaaatta
    24061 tactggataa aaaaacagtt catcaccata atatttctgt tacagcgtaa actccgctgt
    24121 caatcatgag caaaatttac tctgtggcga gataaaactc cggccttacc gggttatccc
    24181 caaagcaacg gcttttttaa catttgtcaa gatgagtgat gacagtttta tggcaagaga
    24241 tgcctgttca gtgactgcca ttgcatttat ctaaccagtt aaaaaataaa agatatttct
    24301 ttgagccgtc tttaacgcca attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt
    24361 tttctaactc taatacacat ggttatccac aggaatagtg gataactgtc tccagcccct
    24421 atcccccgcc gcctgggcaa ttcccacatt cgccgcatga ggcggaagta aaattttttt
    24481 cgtgattatt ttagaattta attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc
    24541 gcaatttaac caaatacagg atagctacaa caaggcaagg tttatgtact ttccggttgc
    24601 cgcattttct ggattttctg caagccaggg gatctctccc agcagcggcg cgggaatcat
    24661 gcgggtgagc gtggtcatat attcagcgtg acgttttccc ggaggcgtaa catcgttcgc
    24721 cacccaaccc gccagagtca gtccggcgtg ttgtattgcc tgcgcagtca acatcgcgtg
    24781 attaatacag ccgagtttca caccaactac cagtatcacc ggcagttgtt cctgtgttac
    24841 ccaatctgca aaagtgaaag tgtcagaaag cggcgtaaac cagccgccag caccttccac
    24901 taacacccag tcagcctgtt gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc
    24961 tatcggcctg ccctcttgcg cgctgatgat gtgcggcgag gtgggttctg cgaaggtgta
    25021 aggatttacc gttgcgtaat ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc
    25081 actattacgt aagccttccg gggtcttttc gctgccagag gcgaccggtt tataacctgc
    25141 cgtccgataa cctactgcct tcgcggcttg taaaagtgca caactggcga cggttttccc
    25201 cacttcggta tccgttccgg tgacaaaata acgtttactc acgagcaatc actcccaaaa
    25261 aaagatgata cgtcagagga tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct
    25321 gcaattgcga acgcgttaat attcgcgggt cgcgcccttc atgaagatgc gtagcaccga
    25381 tgcctttcag cgaacgcatg gcactgagcg catcatcaaa ccatagtgtg atgggctgaa
    25441 tatgatgttg ataatgcacg ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc
    25501 gattagcatg cggacgctcg tccaccgcct gccacgcctg atgcagttcg ggtaacgatc
    25561 cctgcaccag cgtggtaaac gccaccacgc ctccggggcg caccacccga tacagctcgc
    25621 ggagtgccgt ggataaatta ccgcaccact gcactgcgag attgctccat gcaagatcga
    25681 acgtcgcagt cgctaacggc agggattcga tatctcccgc cagataatgg tctgcggcat
    25741 ccttctggcg tgcctgaaca agcattggcg gcgagagatc taaggccgtc acctgcgcgt
    25801 gacgttcccg ccagtggcgg ctcatccagc caggtccaca acccgcgtcc agtacgtggg
    25861 tgtatttacg ctgtgggagc attgccagta agacgtcagc actctggcgc tgtagatctg
    25921 cgtgttgctc atagagtgcg gctgcccgac caaatgccgc tgcaatggct tgtttattaa
    25981 ccgttgccat gcagcacctc cagcagacgg tcgatatcct gcatttcatg cgctgcggtt
    26041 agcgttaagc gcagtcgcgc agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc
    26101 cagcagcctt gctggcgcag tttttctgcc agttgtaacg cacggctgtt atcaccgacg
    26161 atcaatggct ggatggcgct gcatgaatca gccagcgtaa acggcaaatc ctgtactccg
    26221 gcacgaaaac gcgcaatgag tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg
    26281 cgaatgaccg ccagtgacgc acgtaatgcc tgcgcctgag cgggcggcat actggtgctg
    26341 tagataaggt ggcgggcgaa ttgcagcaga taatccgcca ccgtactgga gcaaagcacc
    26401 gcagccccac tgacgccaaa tcctttgcca aaagtcacca ccagcaattc tggttttacc
    26461 ttttgcagcc agcagctgcc gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca
    26521 tcgaccatca accagccatt gtgctgttgc gttacctgct ggatttccgc cagtggcgca
    26581 atatcgccgt ccatgctgaa cacgccttct gtcaccacca gttgctgccc cggacagggg
    26641 gaagcaagca gtcgcgccag atgagtgaca tcgttatgaa caaaacggcg aagctgcgac
    26701 gggcttaaac tggcagcctc cagcaatgag gcatggctaa gccggtcagc gacaatgcgg
    26761 tcctcttttg ccatcatcgc ggcaatgacc gcctgattag cggcaaaacc agagataaat
    26821 agcagtgccc gcgaatagcc cagccactcg gccagttctt cttccagcgc ctgatgcgca
    26881 acgctataac cgctgacgtg accggaaccg ccgctaccga cgccaaattg ctccgcaccc
    26941 tgctgccagg cacggataat ttgcggatga tggcttaaac ctaaataatc gttactggaa
    27001 aagttcagat actggcgatc atccgccacc agccagcgtc cggctccttg cgccactgga
    27061 taacggcgac gcagggcatc ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc
    27121 tgccagctca taatgctgcc gcgttgtaat attcgtcggt gtccggggtc atcagcgcct
    27181 gttcaagacg ttgctgttgt tcgttatccc ctgccagcac ggcagtttgc tgcggattta
    27241 gccccagttt gcggaacagt tgcaggtctt tatcttcttc cggattcggc gtggtcagca
    27301 gtttgcaacc gtagaaaatc gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt
    27361 cgttcatctg ctcgcgtccg gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg
    27421 cgaccgcaat ggtgcgaata aaatcaaagg catcgacatc atcgttatcc gccagcggcg
    27481 tgcctttcac cttcaccagc atgttgattg gtacgctttc cggcggcgtc ggcaggtttg
    27541 ccagttgcag caataatccg gcgcgatctt ttaccgtttc gcctaagccc acaatgccgc
    27601 cagaacagac tttgatcccg gcatcgcgca ctttttccag cgtatcgagg cgttcctgat
    27661 aagtgcgggt ggtgatgata ttgccgtaaa actccggcga ggtgtcgagg ttgtggttgt
    27721 agtaatccag cccggcattc gcaaggcgct gcgcctgaga ttcactcaac gtgcccagcg
    27781 tcatacacgc ctccagcccc atcgctttta ccccctgcac catttgttcc aggtacggca
    27841 tatcgcgttc gtggggattc ttccacgccg cgcccataca gaaacgcgtc gatcccgcag
    27901 ctttcgcttt gcgcgccgac tccagcacct gttcaacttc catcaaccgc tcggcttcca
    27961 gcccggtttt gtagcgcgag ctttgcgggc agtatttgca atcttccgga caagctccgg
    28021 tcttaatcga cagcaaagtg ctgacctgca cctgacgagg atcgaaatgc tgtcgatgca
    28081 cctgctgcgc ttcaaacagc agatccagca acggtttttc aaataattct gtgacttgcg
    28141 acaatgtcca gcgtgggcgg tgagccatgg ggcttctcca aaacgtgttt tttgttgtta
    28201 attcggtgta gaattgtaaa cctaaatctt ttcaatttgg tttacaagtc gattatgaca
    28261 acggacgatc ttgcctttga ccaacgccat atctggcacc catacacatc catgacctcc
    28321 cctctgccgg tttatccggt cgcgagcgcc gaaggttgcg agctaatttt gtctgacggc
    28381 agacgcctgg ttgacggtat gtcgtcctgg tgggcggcga tccacggcta taatcacccg
    28441 cagcttaatg cggcgatgaa gtcgcaaatt gatgctatgt cgcatgtgat gtttggcggt
    28501 atcacccatg cgcccgccat cgagctatgc cgcaaactgg tggcaatgac gccgcaaccg
    28561 ctggaatgcg tttttctcgc ggactccggc tccgtagcgg tggaagtggc gatgaaaatg
    28621 gcgttgcagt actggcaggc caaaggcgaa gcgcgccagc gttttctgac cttccgcaat
    28681 ggttatcatg gcgatacctt tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac
    28741 agcctgtgga aaggctatct gccagaaaac ttgtttgctc ccgccccgca aagccgcatg
    28801 gatggtgaat gggatgaacg tgatatggtt ggctttgccc gcctgatggc ggcgcatcgt
    28861 catgaaatcg cggcggtgat cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg
    28921 taccatccgg aatggttaaa acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg
    28981 attgccgacg agatcgccac cggatttggt cgtaccggca aactgtttgc ctgtgaacat
    29041 gcagaaatcg cgccggacat tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc
    29101 ctttccgcca cgctcaccac gcgcgaggtt gcggaaacca tcagtaacgg cgaagccggt
    29161 tgctttatgc atgggccaac ttttatgggc aatccgctgg cctgcgcggc agcaaacgcc
    29221 agcctggcga ttctcgaatc tggcgactgg cagcaacagg tggcggctat tgaagtacag
    29281 ctgcgcgagc aacttgcccc cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg
    29341 ggggcaatcg gcgtagtcga aaccactcat ccggtgaata tggcggcgct gcaaaaattc
    29401 tttgtcgaac agggtgtctg gatccggcct tttggcaaac tgatttacct gatgccgccc
    29461 tatattattc tcccgcaaca gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag
    29521 gatgaaacat ttttttgcca ataacgagaa gtccgcgtga gggtttctgg ctacactttc
    29581 tgcaaacaag aaaggagggt tcatgaaact catcagtaac gatctgcgcg atggcgataa
    29641 attgccgcat cgtcatgtct ttaacggcat gggttacgat ggcgataata tttcaccgca
    29701 tctggcgtgg gatgatgttc ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc
    29761 ggatgcgcca accggctccg gctggtggca ctgggtagtt gttaacttac ccgctgatac
    29821 ccgcgtatta ccgcaagggt ttggctctgg tctggtagca atgccagacg gcgttttgca
    29881 gacgcgtacc gactttggta aaaccgggta cgatggcgca gcaccgccga aaggcgaaac
    29941 tcatcgctac atttttaccg ttcacgcgct ggatatagaa cgtattgatg tcgatgaagg
    30001 tgccagcggc gcgatggtcg ggtttaacgt tcatttccac tctctggcaa gcgcctcgat
    30061 tactgcgatg tttagttaat cactctgcca gatggcgcaa tgccatctgg tatcacttaa
    30121 aggtattaaa aacaactttt tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa
    30181 aagctgaacg cgaaacgcta aagaccatta atatcaatgt gttacaatat atttagtcta
    30241 aaaaatagac tgcataatac tacaaaacac aacatatcta gtaactatga atcaactact
    30301 tagatagtat tagtgacctg agacagagca ttagcgcaag gtgattttat gtcttcttgc
    30361 gctaattttt tgtcataaaa acatgtggca gactttgtat atctctgctt tgccacgcca
    30421 caccatcatg cattatggct atgcctaaac agaatcaata agaatagaac ataaaaaatg
    30481 acaactctac tagacaatta actacaaaaa tgatagcaat tagtttatat aatataagac
    30541 ttcctatcaa aagttcatcc atatgaagac aaggaattta ttatgccaat cataaagaac
    30601 tgcttatcat caattagta
//
LOCUS       DAZFDP010000041        30387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFDP010000041 DAZFDP010000000
VERSION     DAZFDP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..540
                     /gene="yoaC"
                     /locus_tag="ACHJ1G_001973"
     CDS             241..540
                     /gene="yoaC"
                     /locus_tag="ACHJ1G_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4763833.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(660..839)
                     /gene="yoaH"
                     /locus_tag="ACHJ1G_001974"
     CDS             complement(660..839)
                     /gene="yoaH"
                     /locus_tag="ACHJ1G_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4763834.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            913..2274
                     /gene="pabB"
                     /locus_tag="ACHJ1G_001975"
     CDS             913..2274
                     /gene="pabB"
                     /locus_tag="ACHJ1G_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4763835.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2278..2856
                     /gene="nudL"
                     /locus_tag="ACHJ1G_001976"
     CDS             2278..2856
                     /gene="nudL"
                     /locus_tag="ACHJ1G_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4763836.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHJ1G_001977"
     CDS             3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHJ1G_001977"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4763837.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4535..6133
                     /gene="pdeD"
                     /locus_tag="ACHJ1G_001978"
     CDS             4535..6133
                     /gene="pdeD"
                     /locus_tag="ACHJ1G_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4763838.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6137..7693)
                     /gene="yoaE"
                     /locus_tag="ACHJ1G_001979"
     CDS             complement(6137..7693)
                     /gene="yoaE"
                     /locus_tag="ACHJ1G_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4763839.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7681..7839)
                     /gene="yoaL"
                     /locus_tag="ACHJ1G_001980"
     CDS             complement(7681..7839)
                     /gene="yoaL"
                     /locus_tag="ACHJ1G_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4763840.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8156..9127
                     /gene="manX"
                     /locus_tag="ACHJ1G_001981"
     CDS             8156..9127
                     /gene="manX"
                     /locus_tag="ACHJ1G_001981"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4763841.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9190..9990
                     /gene="manY"
                     /locus_tag="ACHJ1G_001982"
     CDS             9190..9990
                     /gene="manY"
                     /locus_tag="ACHJ1G_001982"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4763842.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10003..10854
                     /gene="manZ"
                     /locus_tag="ACHJ1G_001983"
     CDS             10003..10854
                     /gene="manZ"
                     /locus_tag="ACHJ1G_001983"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4763843.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10909..11367
                     /gene="yobD"
                     /locus_tag="ACHJ1G_001984"
     CDS             10909..11367
                     /gene="yobD"
                     /locus_tag="ACHJ1G_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4763844.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11796..12362
                     /gene="mntP"
                     /locus_tag="ACHJ1G_001985"
     CDS             11796..12362
                     /gene="mntP"
                     /locus_tag="ACHJ1G_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4763845.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12359..13168)
                     /gene="rlmA"
                     /locus_tag="ACHJ1G_001986"
     CDS             complement(12359..13168)
                     /gene="rlmA"
                     /locus_tag="ACHJ1G_001986"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4763846.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13334..13543)
                     /gene="cspE"
                     /locus_tag="ACHJ1G_001987"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13334..13543)
                     /gene="cspE"
                     /locus_tag="ACHJ1G_001987"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4763847.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13556..13699)
                     /gene="yobF"
                     /locus_tag="ACHJ1G_001988"
     CDS             complement(13556..13699)
                     /gene="yobF"
                     /locus_tag="ACHJ1G_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4763848.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(14368..14655)
                     /gene="yebO"
                     /locus_tag="ACHJ1G_001989"
     CDS             complement(14368..14655)
                     /gene="yebO"
                     /locus_tag="ACHJ1G_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4763849.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(14730..14873)
                     /gene="mgrB"
                     /locus_tag="ACHJ1G_001990"
     CDS             complement(14730..14873)
                     /gene="mgrB"
                     /locus_tag="ACHJ1G_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4763850.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            15032..15271
                     /gene="yobH"
                     /locus_tag="ACHJ1G_001991"
     CDS             15032..15271
                     /gene="yobH"
                     /locus_tag="ACHJ1G_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4763851.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(15414..16205)
                     /gene="kdgR"
                     /locus_tag="ACHJ1G_001992"
     CDS             complement(15414..16205)
                     /gene="kdgR"
                     /locus_tag="ACHJ1G_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4763852.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            16382..17755
                     /gene="yebQ"
                     /locus_tag="ACHJ1G_001993"
     CDS             16382..17755
                     /gene="yebQ"
                     /locus_tag="ACHJ1G_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763853.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(17801..18682)
                     /gene="htpX"
                     /locus_tag="ACHJ1G_001994"
     CDS             complement(17801..18682)
                     /gene="htpX"
                     /locus_tag="ACHJ1G_001994"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4763854.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(18874..20922)
                     /gene="prc"
                     /locus_tag="ACHJ1G_001995"
     CDS             complement(18874..20922)
                     /gene="prc"
                     /locus_tag="ACHJ1G_001995"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4763855.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            complement(20942..21640)
                     /gene="proQ"
                     /locus_tag="ACHJ1G_001996"
     CDS             complement(20942..21640)
                     /gene="proQ"
                     /locus_tag="ACHJ1G_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4763856.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(21737..22234)
                     /gene="msrC"
                     /locus_tag="ACHJ1G_001997"
     CDS             complement(21737..22234)
                     /gene="msrC"
                     /locus_tag="ACHJ1G_001997"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4763857.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            22364..23647
                     /gene="yebS"
                     /locus_tag="ACHJ1G_001998"
     CDS             22364..23647
                     /gene="yebS"
                     /locus_tag="ACHJ1G_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4763858.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            23616..26249
                     /gene="yebT"
                     /locus_tag="ACHJ1G_001999"
     CDS             23616..26249
                     /gene="yebT"
                     /locus_tag="ACHJ1G_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4763859.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            26329..27768
                     /gene="rsmF"
                     /locus_tag="ACHJ1G_002000"
     CDS             26329..27768
                     /gene="rsmF"
                     /locus_tag="ACHJ1G_002000"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4763860.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            27886..28122
                     /gene="yebV"
                     /locus_tag="ACHJ1G_002001"
     CDS             27886..28122
                     /gene="yebV"
                     /locus_tag="ACHJ1G_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4763861.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            28227..28418
                     /gene="yebW"
                     /locus_tag="ACHJ1G_002002"
     CDS             28227..28418
                     /gene="yebW"
                     /locus_tag="ACHJ1G_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4763862.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(28419..29075)
                     /gene="pphA"
                     /locus_tag="ACHJ1G_002003"
     CDS             complement(28419..29075)
                     /gene="pphA"
                     /locus_tag="ACHJ1G_002003"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4763863.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(29544..29701)
                     /locus_tag="ACHJ1G_002004"
                     /pseudo
     CDS             complement(29544..29701)
                     /locus_tag="ACHJ1G_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            30030..>30387
                     /gene="nleG7"
                     /locus_tag="ACHJ1G_002005"
     CDS             30030..>30387
                     /gene="nleG7"
                     /locus_tag="ACHJ1G_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO4763864.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHS"
BASE COUNT         7499 a         7629 c         7324 g         7935 t
ORIGIN      
        1 tcgccctctg actagcaact ctggttttct ttcttagctg aataatctgg ttatagcgtg
       61 aggactgacc gttaaatttc tccatcttac tggataacac accatgagct gctaccttta
      121 aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
      181 atgcaaatta aggtcatata tagtcttatc gataacatgg ttaattttaa ggataagaat
      241 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
      301 acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg
      361 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
      421 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg
      481 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga
      541 gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
      601 tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt
      661 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc
      721 tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
      781 atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
      841 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
      901 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
      961 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
     1021 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
     1081 ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
     1141 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
     1201 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
     1261 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
     1321 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
     1381 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa
     1441 gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa
     1501 tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
     1561 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
     1621 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
     1681 ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg
     1741 ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
     1801 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
     1861 gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc cttccctgcc
     1921 gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat
     1981 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
     2041 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
     2101 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
     2161 ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa
     2221 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
     2281 gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa
     2341 attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
     2401 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
     2461 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
     2521 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg
     2581 ccgcccgtcg atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc
     2641 gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc
     2701 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat
     2761 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
     2821 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
     2881 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
     2941 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
     3001 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
     3061 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
     3121 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
     3181 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
     3241 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
     3301 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
     3361 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
     3421 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
     3481 ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
     3541 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
     3601 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
     3661 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
     3721 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta
     3781 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
     3841 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
     3901 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
     3961 agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
     4021 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
     4081 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
     4141 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
     4201 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
     4261 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
     4321 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
     4381 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
     4441 tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
     4501 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
     4561 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
     4621 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
     4681 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
     4741 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
     4801 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactctattg
     4861 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
     4921 accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt
     4981 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat
     5041 cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct
     5101 gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
     5161 tgaagacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa
     5221 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
     5281 gctgctaagt ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt
     5341 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
     5401 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaataatcc
     5461 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
     5521 tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat
     5581 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
     5641 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
     5701 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg
     5761 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
     5821 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
     5881 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
     5941 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
     6001 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
     6061 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
     6121 gccgttacgt taatgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
     6181 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
     6241 tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
     6301 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
     6361 ctgtaccgcc ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag
     6421 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta
     6481 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca
     6541 acaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg
     6601 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acaataccga
     6661 tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt
     6721 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
     6781 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
     6841 cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
     6901 gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc
     6961 gcagcggcaa agtcgactgg tggcgaataa agttgcgacg cgcaatctgg ttaaacactt
     7021 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
     7081 aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca
     7141 ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca
     7201 tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa
     7261 ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
     7321 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
     7381 gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc
     7441 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
     7501 tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt
     7561 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
     7621 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca
     7681 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
     7741 aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gagccagaaa ggtaggcgtt
     7801 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
     7861 ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt
     7921 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
     7981 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
     8041 gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg
     8101 attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac
     8161 cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga
     8221 aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc
     8281 cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt
     8341 gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt
     8401 cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt
     8461 aatggcccgt gatgatgacc caagctttga tgaactggtg gcactggcag tagaaacagg
     8521 ccgtgaaggc gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc aagcacccgc
     8581 tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat
     8641 ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg
     8701 gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac
     8761 cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga
     8821 tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct
     8881 gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc
     8941 tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caggtgaata acgcggtttc
     9001 ggttgatgaa aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga
     9061 agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga
     9121 taagtaacgt attgtgttga ttatcactca gttttcacac ttaagtctta cgtaaacagg
     9181 agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc
     9241 gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc
     9301 ctggtgggta tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa
     9361 atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggct
     9421 tctatcattt ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtatcgca
     9481 ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc
     9541 gttgctttcc agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc
     9601 tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc
     9661 gttgcgctgt ctgttggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg
     9721 gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt
     9781 atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca
     9841 gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc
     9901 tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt
     9961 aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga tacaactcaa
    10021 actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac
    10081 ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg
    10141 gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc
    10201 cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc
    10261 ctggcgctgg aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt
    10321 atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc
    10381 gtacgtccgg tatttgcagc actgggtgcc ggtatcgcga tgagcggcag cctgttaggt
    10441 ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta
    10501 gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa
    10561 aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag
    10621 tggacacatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa
    10681 cacgttacta ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg
    10741 ctgacctttg cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt
    10801 ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt
    10861 tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat gacaatcacg
    10921 gacctggtac tgattctttt catcgccgca ctcctggcct tcgcgatcta cgatcagttc
    10981 atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc
    11041 atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat
    11101 catggtgcgt taataacgac atggttatta agcgcactgg ctctgatggg tttttatata
    11161 ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc
    11221 tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg
    11281 caattagaac agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt
    11341 tataagcttc tcgtttcaac tcaataagtt atgaatttag ccaaagctat gtttagtgta
    11401 tttttaataa tcagacatag cttaggctat attacctctt cccttatttg ttatttattt
    11461 taacgtttca ttgatatata aatctaaatg taaatcgtta tcaataaagc aatgaaataa
    11521 tatattccaa cagttgtttt atattctcaa aatatgttaa ggttgcgccc tcatttgggg
    11581 agtagccgat ttccagattc cggaaatgta cgtgtcaaca tactcgttgc aaaacgtggc
    11641 acgtacggac tgaatacttt cagtcaggcg agaccatatg cacatcaatc gctatgcctg
    11701 catgaacgcg aaaaccatcg cggcttgttg ttcatgtatg catgtttgct gggggcgatg
    11761 atgtgtttta tggatacccc ggtcaggaca ttgtcatgaa tatcactgct actgttcttc
    11821 ttgcgtttgg tatgtcgatg gatgcatttg ctgcatcaat cggtaaaggt gccaccctcc
    11881 ataaaccgaa attttctgaa gcattgcgaa ccggccttat ttttggtgcc gtcgaaaccc
    11941 tgacgccgct gatcggctgg ggaatgggca tgttagccag ccggtttgtc cttgaatgga
    12001 accactggat tgcgtttgtg ctgctgatat tcctcggcgg gcgaatgatt attgagggtt
    12061 ttcgtggcgc agatgatgaa gatgaagagc cgcgccgtcg gcacggtttc tggctactgg
    12121 taaccaccgc gattgccacc agcctggatg ccatggctgt gggtgttggt cttgctttcc
    12181 tgcaggtcaa cattatcgcg accgcattgg ccattggttg tgcaaccttg attatgtcga
    12241 cattagggat gatggttggt cgctttatcg gctcaattat tgggaaaaaa gcggaaattc
    12301 tcggcgggct ggtgctgatc ggcatcggcg tccagatcct ctggacgcac ttccacggtt
    12361 aataagaacg ctgccagagg tgaatattaa agtccgtctg gcagtcgaac acttcttttg
    12421 ctgccagtgt ttgccagact tctggcttcg cacgccaggc aaacggcgtc atctgcaata
    12481 atgcgacggc ttcatcaccg cgaagacgca tcggataaca caactcatca ctctgctgta
    12541 atgtaaaacc ttccagttgt tctgcatgag gtgcatgaag atgtacttca ttgtaaatca
    12601 gccccttcag ctccatcaaa tgtcgcggtc ccggcgtggc agtaatgacc cagccgccgg
    12661 gcttcactac tcgtgctaat tcttctgctt tacacggcgc gtaaatacgt attatggcgt
    12721 ccatactggt atcggaaaac ggcaaacggt ggctggaagc gacacaaaga gtgacctgcg
    12781 gatagcgttt cgccgccgct tttatcgcta ccttcgagac atccaggcca aacgtggtga
    12841 tttcgggcaa cacatcggca aatgcgtgtg tgtaataccc ttcaccacag ccaatatcca
    12901 gcaccgccgt ggccttttca tcaagccgtt cccgcagttg ggcgacaatt gcatcacgca
    12961 gcggctgata atgtccggca tctaagaatg cgcggcgtgc ttgtatcatt tccgcgctgt
    13021 cgcccggatc acgagaccgt ttatgctgaa cgggcagcag attgacatac ccttctttcg
    13081 ccatatcaaa ctgatgtcgc tggggacaga tatagctgtt tttttcacgc gaaagaggct
    13141 gatggcaaag tggacaggaa aacgacatga caactccggc aggatactaa aggccgcaag
    13201 tgtaacgcga atcacgcggc gggcaaatat ccgaacgcca ggagcgaaga taaagcatag
    13261 taaaaagcct cgcattcgac gaggctttat agcgagattg aagcgtattc acacttcaga
    13321 tcagtggatt cgatcagata gctgttacgt taacagctgc cggacctttc tggccgtcct
    13381 gaatttcgaa ctcaacgttc tgaccttcag ccagagtttt gaagccatta ccctggatag
    13441 cggagaagtg tacgaacaca tctttgctgc catcagccgg agtaatgaag ccaaaacctt
    13501 tagactcgtt gaaccactta acctgacctt taatctttgc catttgaaaa attccttaga
    13561 ttgttttctt cgcccgcagg cataacatag ataaaactga cacattactg catgaggcac
    13621 caatataagg ctcggcagag aagcggtatt caacgtcaac gtgtttactc aggacttctt
    13681 tactgaaaat gccacacata aacagaactg tacctcgttt aacccgaaat ctgttatcac
    13741 atacaacgtt aattatggca agccattttt aaacatgtct cgatcagacg cacaaatctc
    13801 gatacgtctt ccactttttt tgcacactta tgcaacggaa tacgcgccga tatatcattg
    13861 tgcttaacct tgccagttca ggcagatact taaactggcg tattttctaa catagttcaa
    13921 taagttcagt tgttccaggc cagtgggaga agttattaca tagtgcgtgc aatatcacat
    13981 tttttgctat gcaatgaata aaaagttata tcactttttc tcataaaaca gtcagttaac
    14041 ggctatcaat taccctaaag agaagtcaat ccccaaaggg attgaaaatt taaaataaga
    14101 aaaattgatg aatgagcaaa aaatcaagag agaaacgttt cgctaataaa taataccacg
    14161 cttaatcaca aatccgatgt aaacatccta ataaattaat gaggaaaata tctttttctg
    14221 atatcccgtt acgcaaataa ataatatgat aattatttga agtcagacca gagttttaat
    14281 ttgaagcagt tgcaccatgg cagtgcgtat aatcaggatg aattgagtaa cccttcaacg
    14341 acggggttac tcaagattga ggaaggatta tcgttcggcg accagacgaa taatatcttc
    14401 gtcttcaacc gatttttgac tctcaacggt ctttttatct gctttctcag gttcgtttgc
    14461 ttcgcacaaa cgacgtaaca gtgcattttg acgtttttgc tgatccagca acgcctcaag
    14521 cagttcaatc tgttcgttag tccgagaact ggcacgattg ataaaaaacc acaagatgag
    14581 cccgataaga agaaccacca ccgatacaac caaagacgca atgttcatca cgcctgaatt
    14641 tacaacttcg ttcatttcac cacctctgag tagaggcgct attctaccac tgctggagag
    14701 gaagaaaatc tagtgctgaa aaaatgatat caccacggga taaactggtt aatggcacaa
    14761 attccgctga aaaattgtac atcctgatcg cacatcatgt tgaatacctg cgcccaaagc
    14821 agcaagcaag ccaacaccac gacaaccaga acgacccatc gaaacttttt cactccaatc
    14881 tccgcttcac ctaccttatg tcattaaact agcatgcgtt agttaaacag cgcctaacta
    14941 tgtcgatatt cgtgcttttt cggaatgagt agcttgcttc agcgcatatt gccgctacga
    15001 ttaagcgaac ataaaaagag aagaggttgt aatgcgattc atcattcgca cagttatgct
    15061 gatagcactg gtatggatag gattattact cagcggatat ggcgttttga ttggaagcaa
    15121 agagaatgcc gcaggattag gtttgcaatg tacctatctg actgccagag gcaccagcac
    15181 ggtacaatac ttgcacacta aaagtggttt cttggggata acagattgcc cactgttacg
    15241 taaaagcaat atcgtggtcg ataatggtta agggtaataa ataaccagat tctcactcat
    15301 gagtaaaata ttcttatagc aagcgcgttt aatataacgc accttcaact ttatttttct
    15361 catgctaaat aaagaacctg tctgtttgat attccgacag gttcttactc tcatcagaac
    15421 ggatagtcgt gataacccat ttgggcagaa attttgcgcg ctgcggtatg caacattgcg
    15481 acatattcct gtaaacgctc ttcagagaaa cgcaacgtcg ggaaggagat gctcaaaccg
    15541 gcaatgacca cgccaaagcg atcaaatacc ggtaccgcaa tgcatcgcag cccttcttcc
    15601 tgctcttcgt tatcttcgcc atacccctgc tcgcgcacct ggtccagaac gggtaataac
    15661 gcttctgtac tggtgatggt ccgctcggta ctgcgtttat actccacgcc ctcaagaatt
    15721 tgcttcactt catcgagatc gcgccatgcc agcagtacct taccaatcgc ggtgctgtac
    15781 agcggattac gacgcccaat ccgtgaatac atgcgcaaat tgtacataga gtcaattttg
    15841 tgaatgtaaa caatactgtc ttcgtccagt gcgccgaggt ggatagtttc tttggtcagg
    15901 cgggagatct cacgcatctg gatatctgcg ctacgaatta aatcgacgtt ttgtaacgcg
    15961 cgagcgccca gttcaaacaa tttcagggtc agggaatatt tctccgactc cccttcctgc
    16021 gccacataac ctaaggtttt catggtctgt aaaaagcgat aaacggtgct ttttgacatc
    16081 atgacgcgct gcgacagctc ggttatccct atttcgcgct cttcacccag cgcctgcaaa
    16141 atgccaaaaa cttttagcac ggaagataca gaatcaggct gtttatccag atctgcgtta
    16201 gccatttatc acctcattgc gagtgtttta taaaaatcag aactgttttt tattataatt
    16261 tcgcaccagg gtggtcgcaa tccatctttt gccggttagt tacaattctg cgacatccac
    16321 cgtgaatatc agtgctagaa tcatacccct gttgattatt caccaaagat ataaaattcc
    16381 tatgccaaaa gttcaggccg acggcctgcc attgccccag cgatacggtg cgatattaac
    16441 cattgtgatt ggcatttcga tggctgtcct tgacggcgca atcgccaacg tcgccctgcc
    16501 aacaatcgcc acggaccttc atgccacgcc agccagatcc atctgggtag tgaacgccta
    16561 tcaaatcgcc attgtcatct ccctgctctc gttttcgttt ctgggcgata tgtttggcta
    16621 tcgacgtatt tataaatgcg gtctggtcgt ttttctgttg tcttcactgt tctgcgccct
    16681 ttctgattcg ctgcaaatgc tcacccttgc gcgtgtcata caaggtttcg gcggtgcagc
    16741 gttgatgagc gttaataccg cacttatccg cctgatctat ccacaacgtt ttctggggag
    16801 agggatgggc ataaactcgt ttattgttgc cgtttcttct gctgccgggc cgacaattgc
    16861 tgcagcaatc ctctccatcg catcctggaa atggttattt ttaatcaacg taccgttagg
    16921 tattatcgcc ctgcttctgg cgatgcgttt tctgccaccc aatggttctc gcgccagtaa
    16981 accccgtttc gacctgccca gcgccgtgat gaacgcgtta accttcggcc tgcttatcac
    17041 tgcgttgagt ggtttcgctc aggggcaatc gctgacgtta attgctgcgg aactggtggt
    17101 aatggttgtt gttggtattt tctttattcg ccgccagctt tctcttcccg taccgctgct
    17161 accggtggat ttactgcgta tcccgctgtt ttcactttct atttgcacat ctgtttgctc
    17221 tttctgcgca caaatgctgg caatggtttc cctgcccttt tacctgcaaa ccgtgctcgg
    17281 gcgtagtgaa gtcgaaacag gtttacttct gacaccgtgg ccgttagcaa cgatggtgat
    17341 ggctccgctg gcaggctatt tgattgaacg cgtacatgca ggattgctgg gtgctttagg
    17401 gttgttcatc atggctgcgg ggcttttttc cctggttctg ctgcccgcgt cacctgcgga
    17461 tatcaatatt atctggccga tgatcttatg tggcgctgga tttggcttat tccagtcacc
    17521 caataaccac accattatta cctccgctcc gcgcgaacgt agcggtgggg ccagtggcat
    17581 gttaggaacg gctcgtctac tgggtcagag tagtggcgcg gcgctggtgg cgctgatgct
    17641 aaatcagttc ggtgataatg gtacgcacgt ttcgctgatg gctgcggcta ttctggcagt
    17701 gattgctgcc tgtgtgagtg gtttacgtat cactcagcca cgatccaggg cataaaaaaa
    17761 gcgcgtcgat caggacgcgc cttttagtat ttacttcata ttacttcagg tattcacccg
    17821 tacgcagagc ttcaattcgt ttatccagcg gcgggtgagt catgaacaac tcactgagcg
    17881 atttcgactt accgttaatg cagaaagcca tcatgctggt tgcttcttgc ggttcatagc
    17941 tggttttcag gcgctgcagc gcggcaatca ttttctcgcg accaaccagt tttgccgaac
    18001 cggcatcagc atggaattca cgatgacgcg agaaccacat ggtgataatg ctcgccagaa
    18061 taccaaacac cagttccaga accgttgcaa ccgcaaagta gatcagcggg ttgccgttgc
    18121 tctcttcacc ttcatcacga tttccgccca taaaacccgc ggcaagctgc gccagaatac
    18181 gggaaataaa gataacgaag gtgttcacca cgccctgaat cagcgtcatg gtaaccatat
    18241 caccattggc gatgtgactg atttcgtgag caattaccgc ctcggcttca tccgggctca
    18301 tgttctgcag caaaccggtg ctgacagcaa ccagagaggc atcacggcgc gcaccggttg
    18361 caaaagcgtt gatgtccggc gcatggtaga tagccacttg cggcatagcg atccctgcct
    18421 gacgagcctg ggttgctaca gtattgacca gccaacgttc cctttcgtta cgcggttgct
    18481 cgatcacttc cccgccaaca gatcgtaatg ccatccattt ggacatcaga agcgaaacga
    18541 aggaaccacc aaaaccgaac agcaaggcca tgatcatcag cccctgaacg ctgctcgact
    18601 gtatccctgt caggctcagt accagcccga aaacgaccat tacggccagg ttcgttagca
    18661 ggaagagcgc gattcgcatc ataattttct ttttacctca gtttaacaaa acgcaatatg
    18721 cgatacccac atcgtatggg ttacgcgact attttcaagt ctggatagtg cgtaagtcac
    18781 cagaaagaca caactttaca ttttgtagca tctgatttac ggcatcttgc cgctgttaaa
    18841 aaatcaggca caatttcttg tgcctgattg atattacttg acgggatcgg gttgttccgc
    18901 gggtctggct ttttcaagct tcgccagatc gagtgcgata ttcaccgtct catccagata
    18961 aggatccggc tcctggtaat ctttcggtag atcatccagc ttcttcaact ccggtttacc
    19021 ttcgcgttta aagcgttcgt tcaaacgcgc cagacgcgtc gcatcatctt cattattctc
    19081 tttctcacgc acagcgtagt tcagagaaac gatattgcgc ttgtccttca tagcgttgaa
    19141 gcgcgcgata tccttcataa tgttctggaa ctcaggatct ttcgcgatac gcgcattatg
    19201 ttccttcagc agctccggcc caaaggccgt taaatcgcct gatttcacat aagtcgcggc
    19261 atcaatgcta tcccacggca gcgcgttatc ttcgaatttc tcacccgttt ccgtttcttc
    19321 attacccgtc ggcatgatga tgtctggcgt tacgccttta cgttgcgtac tgccgccgtt
    19381 aacgcgatag aatttctgga tcgtgtactg cacagaaccc agcgctggcc attcaggacg
    19441 taacatctga tcgtaaatac ggttcaatga acggtattgc tgaacggtgc ctttaccaaa
    19501 cgtcggttca cccacaacca gcgcacgacc gtaatcctgc attgccgcgg caaagatttc
    19561 tgaagccgaa gcactgaaac ggtcaaccag taccaccagc gggcctttat agaacacctg
    19621 cccgtcggta tcgctgtctt cacgaacctt gccgttgtta tcgcggacct gaacaatggg
    19681 acccgcagga ataaacagac ctgagagcga taccgcttcg gtcaacgccc caccgccatt
    19741 gctacgcagg tcgatgatca cactgctgac attctgtttt tccagtttct gcagttgaac
    19801 tttgacatcg tctgtcaaac ccacatagaa gcccggaata tccagcacgc cgactttctc
    19861 tttaccgacg gtcttcaccg acattttaac cgcgcggtct tcgagacgaa tacgttcacg
    19921 ggtcaatgtt acagtgcggg tcttggtccc tttaccagca ggtaaaattt ccagacgaac
    19981 tttactgccc ttcggccctt taattaaggc aaccacatca tcaagacgcc agccaatcac
    20041 gtcaaccatc ggcttgcctg tttgaccaac accgacaatt ttgtcaccaa cgctgatagc
    20101 tttactcttc gctgccggac cacctgccac catcgaattg ataacggtgt agtcatcatc
    20161 catttgcagc actgcgccaa taccttccag cgacaaactc atttcagtgt tgaactgttc
    20221 ggtattacgc ggggaaagat agttggtatg cgggtcgatt tcacgcgcaa acgccgtcat
    20281 tgccagcgag aaaacatctt cgctgttggt ttgcgccaga cgacgaatgg caaatttgta
    20341 gcggcgtgtc agggtttcac gaatttcttt atccgttttt cctgccagct tcaggcttaa
    20401 ctcgtcgaat ttgactttac tgtcccacag cgcgttcaac tcagcctcgt ttttcggcca
    20461 gggcgctttg ctgcggtcaa ggttataagt gtcgttgccg gtgaaatcca tcggcttttc
    20521 cagtaccgac aaagcgtact ggtaacgctc aaaacggcgc ttttgcgcca gattgtagag
    20581 atcgtagaaa acgtcgagtt tgcctgaacg cagttcatcg cctaactcgg ttttcttttt
    20641 cgcgaactgt tcaacatcgc ttgccagcag cacgttgtgg ctgtaatcga gcagattcag
    20701 gtagcggtca aagattttgg ccgaaaatgc ctgatcgagg tcgaactggc gataatgaga
    20761 acgggtgaag cgcgacgtta cgcgctcact taccgtcgca tgctgcgtct cttcctttaa
    20821 taccggaatt tgatcagcac gcgtgatatc ttctacagcg aaggtctggc ctgctattgc
    20881 aagcaggcca gctaacgcgg taagcctaaa aaacatgttc atgcctggcc cggcctccgt
    20941 ttcagaacac caggtgttct gcgcgcacaa tcaaagacat acccgaattc agctggacgc
    21001 ggacgccgtc tttggtgatt tctaatacgg tggcatccat cgcgttttga cccgctttca
    21061 ccttcagggc ttgtccgaca gtcagagctg aaatgtcaga aactggggtg tgctgttctt
    21121 cgcgaggtgc ttttactgtt tttggcgctt tctctaccgg cttttgcgca cgaggtttac
    21181 gttcagcgcc ttctttgcgg cgtggcgtgg tcggacgtgg cttgcgttcg cggcgcggtg
    21241 cgtcttcttt ctcaccagca gctgcggcag cttcgcgttt tttcgcttgc tgttcagcac
    21301 gctgtgcctg aacacgcgct ttcgcttctt caagctgctt gcgagcatgc tctacatgtt
    21361 gctcgtccag ctcaccgcat gggttgccgt caagatcgac acgcgttgcg ccgggtttaa
    21421 caccgtaaag ataacgccag ctcgaagtgt agagacgtaa agcggatcgt aattgcgttt
    21481 tgctcaggtt catttccccg gcaacacgat cgaccaaatc ctgaaaaata ccgattttca
    21541 gcggacgcgc ttcaccttcc gcactgaaac agtggggaaa acgttcggcc agaaacgcga
    21601 ttacttcttt actgctattc aacttaggtt gattttccat gaaatttcct gattacaacg
    21661 gacgtagcca acaagcgcag gcatgaacag gcgtcattat aatgacgcta ccagtaaatg
    21721 ctacgttatc cgttgattat cctgcgacgc tcgcaaagaa ttttttgtaa tccgtcgttg
    21781 caagcacttt ttcaagctgt gccacaagct gacgtaagcc ttgctcgtcc tcgtctgtaa
    21841 agcgaccgaa gacggtacta tcgatgtcaa gaacaccaat aatctgattt ttcaccacca
    21901 gcggcagaac aatttcagaa ttactcgccg catcacaggc aatatgcccg tcaaacgcgt
    21961 gaacatcctc gatacgctgc acttgattgc gggcaaccgc agtgccgcac accccgcgcc
    22021 cgacgggtat ccggacacag gcaattttgc cctgaaatgg tcctagtacc agtgtatcgt
    22081 cctcaagcaa ataaaaacct gcccagttta cgtcagtgag acgctcgtat aacaacgcac
    22141 tggtgttcgc aagcgttgcc agaaaactgg tttctcccgc catcagcgcg ttaaagtcgc
    22201 gatttaaatc cgcgtaaaat tctgttttgt tcattatata atcacttggt tgtcttacct
    22261 ggaactgcca gcctattaaa ataagcatta aatgcgttaa tgctcaagat cattccaatc
    22321 atgggttaat attaatgtta attcttatta catttggcac gtcatggctc ttaacacacc
    22381 acaaattacg ccgacaaaaa agataacagt gagggcaatc ggcgaggaac tgccgcgtgg
    22441 tgattaccaa cgttgcccgc aatgtgacat gctgtttagc ctgcccgaga taaattctca
    22501 tcagagtgcc tattgtccgc gctgtcaggc aaaaattcgc gacgggcgcg actggtcgct
    22561 aacgcgcctg gcggcaatgg ctttcaccat gctgttgctg atgccgtttg cctggggcga
    22621 accgctgttg catatctggc tgttaggtat tcgtatcgac gccaacgtca tgcaaggcat
    22681 ctggcaaatg accaaacagg gcgatacgat aacgggggcg atggtctttt tctgcgttat
    22741 tggtgccccc ctcattctgg tgtcctccat agcatattta tggtttggta accgactggg
    22801 aatgaattta cgtccggtac tgctgatgct tgagcgtctt aaagagtggg taatgctgga
    22861 tatctacctg gtcggcattg gcgttgcttc tataaaggta caggattatg cccatatcca
    22921 ggctggtgtc ggcttgttct cttttgtggc gttggtgatt ttaacgacgg tgacgttgtc
    22981 acatcttaat gttgaggagc tgtgggagcg atattatccg cagcgccccg ctacgcgtag
    23041 ggacgagaaa cttcgtgtct gtcttgggtg ccattttacc ggctatcctg atcagcgtgg
    23101 tcgctgcccg cgttgccata tcccgctacg cctgcgtcgc cgtcatagtc tgcaaaaatg
    23161 ctgggcggcg ctgttagcgt caatcgtttt gttgttacca gccaacctat tgcctatttc
    23221 tatcatttat ctgaacggag gacggcagga agatacaatt ctttccggaa ttatgtcgct
    23281 ggcaagtagc aacattgcgg ttgcgggaat cgtgtttatc gccagtattc tggtaccgtt
    23341 tactaaagtg atcgtcatgt tcactttact gttgagcatt cattttaaat gccagcaagg
    23401 tttacgcaca cgcattctgt tactgcggat ggtgacctgg attggacgct ggtcgatgct
    23461 cgacctgttt gtcatatctt taaccatgtc gctgattaat cgcgatcaga tcctcgcttt
    23521 tactatggga ccggctgcgt tttattttgg cgcagccgta attttgacta ttcttgctgt
    23581 ggaatggctg gacagccgct tactttggga tgcacatgag tcaggaaacg cccgcttcga
    23641 cgactgaagc gcagattaaa aataaacgcc gtatctcacc tttctggctg ctgcctttca
    23701 tcgcgctaat gattgccggt tggctgattt gggacagtta tcaggaccgg ggtaataccg
    23761 tcaccatcga ctttatgtcg gcggatggta ttgttccggg ccgtacgcct gttcgttatc
    23821 agggcgttga agtcggaaca gtgcaggata tcagcctcag cgacgatctt cgtaagattg
    23881 aagtcaaggt cagcatcaag tccgatatga aagatgcgct gcgcgaagag actcaattct
    23941 ggctggtgac gccaaaagca tcgttggcag gtgtctccgg gctggacgcc ctcgtcggtg
    24001 ggaactatat cggcatgatg ccgggtaaag gtaaagagca ggatcacttt gtcgcactcg
    24061 atacccaacc gaagtatcgg ctggataacg gcgatctgat gatccacctg caagctcccg
    24121 atctcggttc gctgaacagc ggttcattgg tctatttccg caagatcccg gtgggaaaag
    24181 tctacgacta tgccatcaat cccaacaagc aaggcgtggt gattgatgtc ctgatcgagc
    24241 ggcgttttac cgatctggtg aaaaaaggta gccgtttctg gaacgtttcc ggcgttgatg
    24301 ccaacgtcag tgtcagtggc gcgaaggtga aactggaaag tctggcggca ctggttaacg
    24361 gtgcgattgc cttcgattca cctgaagagt cgaaacctgc cgaggcggaa gatacctttg
    24421 gtctgtatga agatctggcc cacagccagc gtggcgtaat aataaaactg gaactgccga
    24481 gtggggccgg attgaccgcc gactcgacgc cgttaatgta tcaggggctg gaagtcggac
    24541 agctaactaa actggattta aatcctggtg gtaaagtcac tggggaaatg acagttgatc
    24601 ccagcgtcgt taccctgctt cgtgaaaata cccgcatcga attacgtaat ccgaaattat
    24661 cccttagcga tgccaatctc agcgccctgc tgactggcaa aaccttcgag ttggtacccg
    24721 gcgatggcga gccacgcaaa gagttcgttg ttgtgccagg cgaaaaagca ctgctgcatg
    24781 aacctgatgt tctgacgctg accctgaccg cgccggaaag ttacggtatt gatgctggtc
    24841 agccgctcat tcttcacggc gtgcaggtag gccaggttat cgatcgtaaa ctcaccagca
    24901 aaggcgtcac ctttaccgtc gccatcgagc ctcagcatcg agaactggta aaaggcgata
    24961 gcaaatttgt cgtcaacagc cgtgtcgacg tgaaggtggg gctggatggc gttgagtttc
    25021 tcggtgccag cgcctcagaa tggattaacg gcgggatacg tattctgccg ggcgataaag
    25081 gcgagatgaa agccagctat ccactgtatg ccaatctgga aaaagcgctg gagaacagcc
    25141 ttagcgattt acccaccaca accgtgagtt tgagtgcaga gacgctgccg gatgtgcagg
    25201 caggatcggt agtgctctac cgtaaatttg aagttggtga agttattacc gtccgtccgc
    25261 gagctaacgc gtttgatatc gatctgcata ttaagccgga gtatcgcaac cttctgacca
    25321 gcaatagcgt gttctgggca gaaggcgggg cgaaagttca gctgaatggt agtggcctga
    25381 ccgtacaggc atccccgctc tccagagcat taaagggagc cattagcttc gataacctca
    25441 gcggtgccag cgccagtcag cgtaaaggcg acaaacgaat tctgtatgct tccgaaacag
    25501 cggcccgtgc ggttggtggg cagattacgc ttcacgcttt cgatgccgga aaactggcgg
    25561 tcgggatgcc aattcgctat ctcggtattg atatcgggca aatccagacg ctggatctga
    25621 ttaccgctcg caatgaagta caggcaaagg cggtgctcta tccggagtat gtccagacct
    25681 tcgcccgcgg cggtacgcgc ttctcggtgg tcacaccgca aatttcagcc gcgggcgttg
    25741 agcatctaga tactatcctc cagccgtata tcaacgtcga accaggccgg ggcaatcctc
    25801 gccgcgactt tgaattacaa gaggccacca ttactgattc gcgttacctg gatggcttaa
    25861 gcattattgt tgaagcgccg gaagccggtt cgttaggtat tggtacgcct gtgctgttcc
    25921 gtggtctgga agtcggtacg gttacaggaa tgacgctggg gacattgtca gatcgcgtga
    25981 tgattgcgat gcgcatcagt aaacgctatc aacacctggt gcgtaacaat tccgtcttct
    26041 ggttggcatc gggttacagt ctggactttg gtctgacggg cggcgtagtg aaaaccggca
    26101 cctttaacca gtttatccgt ggcggcatcg ccttcgccac gcctccgggt acgccactgg
    26161 caccgaaagc ccaggaaggc aaacacttcc tgttgcagga aagtgaaccg aaagagtggc
    26221 gtgaatgggg aactgcgctt cccaaataat gcccactgct ccggcgtgcc tgcgccggag
    26281 cgtttatgct aaactgcgcg cctgtttttt tgccagtggt acatgctcgt ggcccaacac
    26341 accgtttatt tcccggacgc ctttctgaca caaatgcgcg aagcgatgcc ttcgacgctc
    26401 tcatttgatg attttcttgc cgcctgtcag cgcccgttgc gccgcagcat tcgcgttaat
    26461 acgctgaaaa tctccgttgc tgatttcctg caattaaccg ctccttatgg ctggacgctt
    26521 acgccaattc cgtggtgtga agaaggtttc tggattgaac gcgacaatga agatgcattg
    26581 ccattgggta gtaccgccga gcatttaagc ggcctgtttt atattcagga agccagttca
    26641 atgttgcccg ttgccgcctt gtttgctgac gataatgcac cacagcgggt gatggatgtc
    26701 gcagctgcgc caggctccaa aacgacgcaa attgccgcgc ggatgaataa cgaaggggca
    26761 atccttgcca atgagttttc cgccagtcgg gtaaaagtgt tacatgccaa tatcagccgc
    26821 tgtggcatca gtaatgttgc gctcacacat tttgatggcc gcgtgtttgg tgcggcagtg
    26881 ccagaaatgt tcgatgccat tttgctggac gctccctgct ctggcgaagg cgtggtgcgt
    26941 aaagatcccg atgcgctaaa aaactggtca ccagaaagca atcaggaaat cgcagctaca
    27001 caacgggagc ttatcgacag cgcctttcat gcattacgtc ctggtggtac gctggtttac
    27061 tcgacctgta ccttaaacca ggaagaaaac gaagccgttt gcctgtggct gaaagagact
    27121 taccccgacg cagtagagtt tttaccactt ggcgatctct tccctggtgc aaacaaagcg
    27181 ctgaccgaag aaggcttttt gcatgttttc ccacaaattt acgactgcga aggcttcttc
    27241 gttgctcgtc tgcgtaaaac tcaggcgatt cccgccttac ccgcccccaa atacaaagtc
    27301 ggtaattttc cgttcagccc ggtgaaagat cgcgaagctg gacaaattcg tcaggcgact
    27361 gcaggtgttg gcttaaactg ggatgaaaac ctgcgcctct ggcagcgtga caaagaactg
    27421 tggttgttcc cggtgggcat tgaagccctg atcggtaaag tccgattttc tcggttgggg
    27481 attaaacttg ccgagacgca caacaaaggt tatcgctggc agcatgaagc ggttattgct
    27541 cttgcctcac ccgataatat gaatgcattc gagctgacac cgcaggaagc ggaggagtgg
    27601 tatcgcgggc gcgatgttta cccgcaagcc gcgccagtgg cggatgatgt attggttact
    27661 ttccagcatc aaccgattgg tttagccaaa cggattggtt cgcgattgaa aaacagctat
    27721 ccgcgtgaac tggtgcgcga tgggaaactt tttaccggta acgcctgaca gctcacaaaa
    27781 aaagcgcact ttttgactgg cacattcggc tgcctcaact aggctgaaaa atggtgcgat
    27841 cggactggtc gtaccacaat cggcagctaa atggagagca cgaagatgaa aaccagtgtg
    27901 cgcataggcg cttttgaaat cgacgacggc gaattacacg gtgaatcgcc gggtgatcga
    27961 acgttaacca ttccttgtaa atctgacccc gatttatgta tgcaactcga tgcctgggat
    28021 gctgaaacca gtatccctgc cctgctaaat ggcgaacact ctgtccttta ccgtacccgt
    28081 tacgatcaac aatctgatgc ctggattatg cgtcttgcct gatccaaaaa gaacccgtcg
    28141 gcatggcggg ttatttgtcc tggttattcc cccgttgtaa aatctctcct aaacttaacg
    28201 gtacggcacc acacttcggg gatgaaatgt tcgcgctggt actttttgtt tgctacctgg
    28261 atggcggttg tgaagatatt gttgtggatg tctacaacac ggaacagcag tgtctttatt
    28321 ctatgagcga tcaacggatc cgccatggcg gttgttttcc gattgaggat tttatagatg
    28381 gtttctggcg accagcacag gagtatggtg atttttaatt attgcaattg cacaagagtc
    28441 agttcgcccc caaagacagc accggtatca atataatgca ggttgccaat atccacgcga
    28501 tgtcgcaacg gtgtatgacc aaaccagaaa tgatcagcac ctgtaattcc ctgccctttt
    28561 tggcgttcac ctaatcgcga gcggctccac aagacctgat gcaaatcaac gtccttttgc
    28621 cattcataaa catcatctgg ataatcggca tgagcaataa catgtttgcc ggtacgactg
    28681 tgtacttcaa gaataaaggg caaatgctga catttttcca gcgccgtttt cgcttgtttc
    28741 tgttgattat ctgccagcgc aataaaccag tcgccgccat tcatcaacca caaagacatc
    28801 tgctgggatg ccagcgcatc catcgccatc tgttcatgat tgcctcttac cgcacaaacc
    28861 caatgttgtt ccagtaactg cagacaacgt aaactttgcg gcccacgatc gataacgtct
    28921 cctactgaga taagtaaatc tcgccacgga tcaaaacgac aatgccataa tttgcggcgc
    28981 aactgctcaa gacaaccgtg tatatcgcca gaaagccaga tatgtcgcca ttgatgaccc
    29041 gcaattctct gataaacggg cgcaggctgt ttcatcaata ttttcctccc gcgctaaaga
    29101 tcacataatc ttaacaagaa tgttaaaaaa cgctggactc agacagtaga gtgtgtgtta
    29161 tggttgacta taaagtcagc gaaggaaatg cttctggctt ttaacagata aaaagagacc
    29221 gaacacgatt cctgttttcg tcaacaaaca acaaaatctt ttagaattaa tgtgttaaat
    29281 aaatttatgc tcatctttta atctctaaca cgtactatta catattaata cattcaatta
    29341 gttaccattt ttttcgagtt ttttagagaa attttcggga atatttcagg tcaatccatg
    29401 catacacaag caattcctgt attgaataat tccgtagcaa ttatgtaaaa tcatctccgg
    29461 ctgattttca ttcaaactcg cgctatcgaa cgtccatcag ccagccgtgg cacgttcttg
    29521 catacgacgt gccgcggatc ctattattct agtatgaaat ccctaaccga tttcaaattc
    29581 tccagactgc tcagattctc ttttatccca cgctggcgct gataaatctc gtcattgcaa
    29641 tcgacctgcg ctatcgctgc cgcagttctt ccagtttctg catcgataac tccatcttct
    29701 gagcaataaa cctcacctat gcaatatttt atgtcaatta tttcaattgc attaatatct
    29761 tgaatggatt acatagagtt aatgtatcca gtactcccta ttctcttgca tataatacat
    29821 gttgcaactt acatctcagc gctatgaaaa aacaccacct ctctctttat gaaattctgg
    29881 atttaccaag cgctaattta tcatttcaat ctacctttaa gcattgcatt tatctcccta
    29941 cgaggtcata ctttaggaag ttaaatatga atgataatat acctacagcg cgaaatcaca
    30001 aacaatcgac ttgtattaca gaaaaaaaca tgcctatctt tttaaacttc acagcaggca
    30061 gtatcttacc tgagaatgag ctagcatctt tacgttatat tgtgcagcaa aatcaaaatg
    30121 atactgtaat cataaaagaa cgttataaaa tggatatccg ttatatcgaa tcagtcaatg
    30181 gttttacagt aaatcctgta tgcagtaatc atttctccat atttatggcg agacaaaaca
    30241 ctattgctcg caacctggaa cagcagatca acaacggacg aagttttgca caaatatctc
    30301 aggattttat gcttcaatta tcttcaaata taggatggaa aaaaggggcc gaaaacgccc
    30361 ttaaaaataa aatccattct cattcat
//
LOCUS       DAZFDP010000042        30175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFDP010000042 DAZFDP010000000
VERSION     DAZFDP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..1681
                     /gene="yegH"
                     /locus_tag="ACHJ1G_002006"
     CDS             98..1681
                     /gene="yegH"
                     /locus_tag="ACHJ1G_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4763865.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(1955..3808)
                     /gene="asmA"
                     /locus_tag="ACHJ1G_002007"
     CDS             complement(1955..3808)
                     /gene="asmA"
                     /locus_tag="ACHJ1G_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4763866.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3830..4411)
                     /gene="dcd"
                     /locus_tag="ACHJ1G_002008"
     CDS             complement(3830..4411)
                     /gene="dcd"
                     /locus_tag="ACHJ1G_002008"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4763867.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4503..5144)
                     /gene="udk"
                     /locus_tag="ACHJ1G_002009"
     CDS             complement(4503..5144)
                     /gene="udk"
                     /locus_tag="ACHJ1G_002009"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4763868.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5462..8772
                     /gene="dgcE"
                     /locus_tag="ACHJ1G_002010"
                     /pseudo
     CDS             5462..8772
                     /gene="dgcE"
                     /locus_tag="ACHJ1G_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8811..9668)
                     /gene="alkA"
                     /locus_tag="ACHJ1G_002011"
     CDS             complement(8811..9668)
                     /gene="alkA"
                     /locus_tag="ACHJ1G_002011"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4763869.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9801..11153
                     /gene="yegD"
                     /locus_tag="ACHJ1G_002012"
     CDS             9801..11153
                     /gene="yegD"
                     /locus_tag="ACHJ1G_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4763870.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11186..13105)
                     /gene="yegI"
                     /locus_tag="ACHJ1G_002013"
     CDS             complement(11186..13105)
                     /gene="yegI"
                     /locus_tag="ACHJ1G_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4763871.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(13102..13863)
                     /gene="pphC"
                     /locus_tag="ACHJ1G_002014"
     CDS             complement(13102..13863)
                     /gene="pphC"
                     /locus_tag="ACHJ1G_002014"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4763872.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13860..14519)
                     /gene="yegL"
                     /locus_tag="ACHJ1G_002015"
     CDS             complement(13860..14519)
                     /gene="yegL"
                     /locus_tag="ACHJ1G_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4763873.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14740..14799)
                     /gene="ibsA"
                     /locus_tag="ACHJ1G_002016"
     CDS             complement(14740..14799)
                     /gene="ibsA"
                     /locus_tag="ACHJ1G_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4763874.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15072..15128)
                     /locus_tag="ACHJ1G_002017"
     CDS             complement(15072..15128)
                     /locus_tag="ACHJ1G_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4763875.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15400..15456)
                     /locus_tag="ACHJ1G_002018"
     CDS             complement(15400..15456)
                     /locus_tag="ACHJ1G_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4763876.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15735..16982
                     /gene="mdtA"
                     /locus_tag="ACHJ1G_002019"
     CDS             15735..16982
                     /gene="mdtA"
                     /locus_tag="ACHJ1G_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4763877.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16982..20104
                     /gene="mdtB"
                     /locus_tag="ACHJ1G_002020"
     CDS             16982..20104
                     /gene="mdtB"
                     /locus_tag="ACHJ1G_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4763878.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20105..23182
                     /gene="mdtC"
                     /locus_tag="ACHJ1G_002021"
     CDS             20105..23182
                     /gene="mdtC"
                     /locus_tag="ACHJ1G_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4763879.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23183..24598
                     /gene="mdtD"
                     /locus_tag="ACHJ1G_002022"
     CDS             23183..24598
                     /gene="mdtD"
                     /locus_tag="ACHJ1G_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4763880.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24595..25998
                     /gene="baeS"
                     /locus_tag="ACHJ1G_002023"
     CDS             24595..25998
                     /gene="baeS"
                     /locus_tag="ACHJ1G_002023"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4763881.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25995..26717
                     /gene="baeR"
                     /locus_tag="ACHJ1G_002024"
     CDS             25995..26717
                     /gene="baeR"
                     /locus_tag="ACHJ1G_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4763882.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26908..27240
                     /gene="yegP"
                     /locus_tag="ACHJ1G_002025"
     CDS             26908..27240
                     /gene="yegP"
                     /locus_tag="ACHJ1G_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4763883.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27449..27745
                     /locus_tag="ACHJ1G_002026"
     CDS             27449..27745
                     /locus_tag="ACHJ1G_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4763884.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27747..28043
                     /locus_tag="ACHJ1G_002027"
     CDS             27747..28043
                     /locus_tag="ACHJ1G_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4763885.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28146..29507
                     /gene="yegQ"
                     /locus_tag="ACHJ1G_002028"
     CDS             28146..29507
                     /gene="yegQ"
                     /locus_tag="ACHJ1G_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4763886.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29837..30154)
                     /gene="yegR"
                     /locus_tag="ACHJ1G_002029"
     CDS             complement(29837..30154)
                     /gene="yegR"
                     /locus_tag="ACHJ1G_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO4763887.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6972 a         8049 c         7969 g         7185 t
ORIGIN      
        1 atgagataaa tagtgctgca acattgcatt tttgccccga tttatccatg atcgaattat
       61 gacatttgtc atacaacgaa taggttttgt acttactatg gaatggattg ccgatccgtc
      121 tatctgggcc gggttaatca cgctgattgt gatcgaactg gtcctcggca ttgataacct
      181 ggtctttatt gccatcctcg ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt
      241 taccgggctg ctgctggcga tgttaatgcg cctgttactg ctggcgtcaa tctcctggct
      301 ggtcaccctg actcaaccgc tgttcagctt ctgctcgttt acctttagcg cccgcgactt
      361 aatcatgctg tttggtggtt tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg
      421 gctggaaggg aaagacagca ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt
      481 ggtgacgcaa attgtggtac tggacgccat cttctcactc gactcagtga ttaccgccgt
      541 cgggatggtc gaccatttac tggtcatgat ggccgccgtg gttatcgcta tcagcctgat
      601 gttgatggcc agcaagccgt taacgcaatt tgttaacagt cacccgacca tcgttattct
      661 ctgcttaagc ttcctgttga tgattggctt tagcctggtg gcagaaggtt tcggcttcgt
      721 cattccgaaa ggctacctgt acgctgccat tggtttctcg gtgatgatcg aggcgctcaa
      781 tcagttggct atctttaacc ggcgacgttt tctttccgct aaccagacgc tgcgccagcg
      841 gactaccgaa gcggtaatgc gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc
      901 cgaaaccgcg tccatgctgg tggatcatgg taaccagcag atctttaatc cgcaggaacg
      961 gcggatgatt gagcgggtac ttaatcttaa ccagcgtact gtcagcagca ttatgacgtc
     1021 gcgccacgat attgagcata tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct
     1081 ggagcgaaat cagcatacgc ggctggtggt taccgacggt gatgacgcag aagatttgct
     1141 cggtgttgtt cacgttatcg acctgctaca acagtcactg cgcggcgaac cgctcaacct
     1201 gcgggtgttg attcgccagc cgctggtgtt cccggaaacc ttgccgttgt tacctgccct
     1261 ggagcagttc cgtaatgccc gcacgcactt tgcttttgta gtggatgagt ttggctcggt
     1321 ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt gccggtaact taccgaacga
     1381 agtggaagag atcgacgccc gccatggtat tcagaagaat gcggacggtt cctggacggc
     1441 gaatggtcat atgccgctgg aagatctggt gcaatatgtg ccgctgccgc tggatgaaaa
     1501 acgtgaatat cacaccattg ccgggctgtt gatggaatat ttacagcgta ttccaaaacc
     1561 tggcgaagaa gttcaggtgg gggattattt gcttaaaacg ttgcaggtgg aaagccatcg
     1621 cgtgcagaag gtgcagatta ttccgctgca taaggatggc gagttggagt acgaggtgtg
     1681 atggtatcgg tcattttacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     1741 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     1801 ttcaccggct tgagcgttcc atagtagtga ggaacatcaa gtgaacggaa cagccttcga
     1861 tcttacatat tcagaatata atggtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     1921 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     1981 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     2041 cttcgtcctg tagatgtttg cgcagtaact gatccacttg cagactgtaa ttgagttgct
     2101 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
     2161 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
     2221 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
     2281 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agacgcgtta
     2341 cgttatcgaa gttttcagcg gccttcacat caccaccatt acgttctacc gcttgctgaa
     2401 tcatctgctg gaagttcatc ccttccatgc gcgtgtcggt catttcgaca tgtgcctgtc
     2461 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
     2521 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
     2581 caacgttttc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
     2641 tgcccggcag tgaaacctgt ccaccgttaa gcttgccctg cagttgagta atttccagca
     2701 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
     2761 cgttactggc ctgcaacaaa atatcagccg taaagccttg cagtccctga tatgccggtt
     2821 catcaatacg cgaagaaatg accgggcgcg gcaatgttga ttggctctgc ccctgctgcg
     2881 cggcaccgtt ttcaccattc gctgtttcat taagcgggat gaggttgtca agattcagtt
     2941 gcgggaattg cagtctcagc tgccattcag gtttctctgt cagcgtgacc tgtgcttgcc
     3001 cgctcagcgt gctgtcattg gcggtcaagc tgatttggtt aaatgaaagg cgtttatgtg
     3061 actcctgcca ctgggcctgg aaactcccct gcccctgaat accttgtttt ggtaaatctg
     3121 caccctgcaa ctgccagtta atttgttcaa tagccgccgt taaatcatgc ggataatcag
     3181 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
     3241 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
     3301 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag cacgctgtcc gccaccttaa
     3361 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
     3421 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
     3481 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
     3541 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
     3601 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
     3661 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
     3721 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaggagta
     3781 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
     3841 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
     3901 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
     3961 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
     4021 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
     4081 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agcgtcaccg
     4141 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
     4201 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
     4261 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
     4321 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
     4381 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgcg catcactccc
     4441 ttaacgccaa ttacgtcaag ggcataccag gttatcactg aacggtacac aatttatcaa
     4501 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
     4561 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
     4621 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
     4681 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
     4741 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
     4801 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
     4861 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
     4921 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
     4981 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
     5041 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
     5101 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
     5161 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
     5221 atccgcctga taccagtcga atagcgctgc cgcgcgctca gagttaattg ttgacgaaga
     5281 attcccggtg gcaaattacg ttgatcagtt ttatacaagg taaaaaatgt tatacgcagt
     5341 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
     5401 tttgtactag cataaacaca gaaacgaata ctggcgacct ggtcttgcgg ataaagcggt
     5461 aatgagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
     5521 cagtttaggt ttagtctcgt ttatctttac ccttttctcg cttgagcttt cgcagtttgg
     5581 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
     5641 tgccgggcgc atgtggccgg gaattgcgct gagctgctcg ctgggaaata tcgccgcatc
     5701 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
     5761 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
     5821 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgctaggggg
     5881 tgttctggtt gtcctgctga cgcccggaga cgatcctctc agggcatttt tgatatgggt
     5941 actgtcagaa tccatcggcg ctctggcact ggtgccgctg ggattgttat ttaaaccaca
     6001 ctatctgctg cgccatcgca acccacggtt gctttttgag tcgctgctca cgttagccat
     6061 cacactgacg ttaagctggc tttcgatgct gtatctgccg tggcctttta ctttcattat
     6121 tgtgctgttg atgtggagcg ccgtgcgcct gccacgaatg gaagcctttt tgatcttcct
     6181 taccacggtg atgatggtgt cgctgatgat ggccgcggat ccctccctgc ttgctacgcc
     6241 gcgtacatac ctgatgagcc atatgccgtg gctaccgttt ttgctgatcc tgctgcccgc
     6301 caacatcatg acgatggtga tgtatgcctt tcgtgcggaa cgcaaacaca tttccgaaag
     6361 cgaaacccgt tttcggaacg ccatggaata ttccgctatc ggtatggcgt tagtgggcac
     6421 cgagggacaa tggctgcaaa ccaacaaagc gctctgccag tttctcgggt acagtcagga
     6481 agagctgcgc ggactcacct ttcagcaact gacctggccg gaggatctca ataaagatct
     6541 ccaacaggtt gaaaagctga taagcggtga aataaacacc tattcaatgg aaaaacgcta
     6601 ctacaaccgc aatggcgatg ttgtctgggc gttgcttgcc gtctcactgg tgcgccacac
     6661 ggatggcacg ccgctctatt ttatcgctca gattgaagac attaacgagc taaaacgcac
     6721 cgaacaggtg aatcagcaac tgatggagcg catcacgctg gctaacgaag cgggcgggat
     6781 tggcatctgg gagtgggagt tgaagccgaa tatttttagc tgggataagc ggatgttcga
     6841 gctgtatgaa attcctccac atatcaaacc gaactggcag gtgtggtacg agtgcgtgct
     6901 gccggaagat cgccagcatg ccgaaaaagt gattcgttgc aatcacgctc gccctttaaa
     6961 ctggaatttc gcattaccgt aaaagacggt attcgccata tccgcgccct cgccaaccgg
     7021 gtactgaata aagaaggcga agtcgaacgc ctgctcggca ttaatatgga tatgactgag
     7081 gttaaacagc ttaacgaggc attgtttcag gaaaaagagc gcctgcacat aacgctggac
     7141 tccatcggcg aagccgtggt ctgtattgat atggcaatga aaattacctt tatgaatcca
     7201 gtggcggaga agatgagcgg ctggacgcag gaagaagcat taggtgttcc gctcctgacg
     7261 gtgttgcata ttacctttgg cgacaatggt cccctgatgg agaacattta cagtgccgac
     7321 acctcacgtt ccgcgattga acaagatgtg gtgttgcact gccggagcgg cggcagctac
     7381 gacgtgcatt acagtattac gccgttaagt actctggacg gcagcaatat tggttcggtt
     7441 ctggtgattc aggacgttac cgaatcacgc aaaatgctgc gccagctgag ctacagcgcc
     7501 tcccatgatg cactgacgca tctcgccaat cgcgccagtt ttgagaagca actgcgtatg
     7561 ctgctgcaaa cggtaaacag tacacatcag cgacatgccc tggtgtttat cgatcttgat
     7621 cgctttaaag cggtgaatga cagcgccggg catgcggcgg gcgacgcttt gctgcgcgaa
     7681 ctggcgtcgt tgatgctgag tatgctgcgt tccagcgacg tgctggcgcg actcggtggg
     7741 gatgaatttg gtctgctgct accagactgt aatgttgaaa gcgcgcgttt tatcgctaca
     7801 cgcattatca gtgccgtgaa tgactatcac tttatatggg aaggccgtgt gcatcgggta
     7861 ggtgccagtg ccgggattac cttgattgat gacaacaatc atcaggcggc agaagtgatg
     7921 tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg gacggggccg ggtgacggtt
     7981 tacgaaccac agcaagctgc cgcacatagc gaacgggcgg cgatgtcgct tgatgaacag
     8041 tggcggatga ttaaagagaa tcagttgatg atgatggccc acggtgtcgc ttcgccgcgg
     8101 atcccggaag cgcgtaattt gtggctgatt tcgcttaaac tctggagttg cgaaggcgag
     8161 attattgatg aacaaaaatt tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt
     8221 gaccgacggg tattccacga atttttccag ctggccgcaa aagcggttgc cagtaaaggc
     8281 ataagcatcg ccctccccct ttccgttgcc ggtttgagta gcgccacgct ggtgaatgat
     8341 ctgcttgagc agctggaaaa tagccctcta ccaccacggt tattacatct gattattccg
     8401 gcagacgcga ttttagatca cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg
     8461 atagtgctca gccaggtggg ccgcgatctg caaatcttca actcgctgaa agcgaatatg
     8521 gcagattacc tgctacttga tggtgagtta tgcgccaacg tgcagggtaa tttgatggat
     8581 gagatgctga ttacgattat tcaggggcac gctcagcgac tcgggatgaa aaccatcgcc
     8641 gggccagtcg ttttaccctt agtgatggat acgctttctg gcatcggcgt cgatctgatt
     8701 tatggtgatg tgattgccga tgcccaaccg ctggatttgc tggtgaatag cagttatttc
     8761 gcgattaact gagggagcgt ttgcccattg cctgatgcga cgcatcaggc ctacaattcg
     8821 tccgttccgt ctggttgcca gccttccgta taccagatat gcaacagcgc ataagaacgc
     8881 cagggcttcc agcgctcggc ataacggcgg atttgtgccg gtgtcatccc cggaaatcgc
     8941 tgtttaatca gataatcatc cggcagaaaa acatctttcg cctgccagcc acgcaaagcg
     9001 aaataattcg ccgtccagcg cccgataccc ggaaaagttt gcagcgtttt catcgcctgc
     9061 tccacatcgc ccggaattgt cattggtaag ctgccctcca gcgccgcatt tgccagatga
     9121 atcagtgcct ctgcccgttt caacggcatg cctaacgctt ttaatgcctg cgggtcggct
     9181 gctgccagcc gctgaggcgt ggggaagcag atatactccg gaaaatcatc cagccgttcg
     9241 ccataaagct gtacaacttt ggcagtcaat tttgccgcca tcgccacgct caccagttgg
     9301 cctaaaatcg cccgcacacc ctgctcaaaa gcatcaatac agccgggtaa acgcaatccg
     9361 ggccgcgccg cgcctaactt gcccaacgca ccgttaacaa tctgcgggtt acattgcaga
     9421 tcaaacaggc ggctcatttt cgccagacac tcagcggcaa cgggttctaa acctgcactt
     9481 aaatttatgt gcagagtatg gcgggctata tccggaatag cagtcaccac gccgcgatat
     9541 tcgcccaccg ccagactacg ggcataataa tcctcagcga ccgtttccac accactcact
     9601 gcacgggcgg cgagaaatcc caacatccac gaccagtcat acggcggctg ccagttcagg
     9661 gtatacatcg catctcctta ttcatccgct ttcagcataa acgttattca gacgctacgc
     9721 tttgctttca tattccggtt gtcgcgacag caacatttcg ctaaagtcac gcccttcttc
     9781 accggcatgg ggattaattc gtgtttattg gttttgatta cggtacagca aactgttcag
     9841 tggcggtcat gcgtgacggt aaaccgcaat tactgaaaat ggaaaacgac agcacgctgc
     9901 tgccttcaat gctttgcgcg ccaacgcgtg aatcggtaag cgaatggctg taccgccatc
     9961 atgatgttcc ggcagacgac gatgaaacgc aggcgctgtt acgtcgggcg attcgttata
    10021 accgcgaaga agatatcgat gttacggcga aaagcgtgca gttcggtctt tcctcactgg
    10081 cacagtacat tgatgatcca gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg
    10141 gtgccagcgg cttaaaaccg cagcaggtag cgctgtttga ggatctggtc tgcgcaatga
    10201 tgttgcacat tcgccagcag gcgcaggcgc agctgccaga agcgattact caggcggtga
    10261 ttggtcgccc gatcaacttc caggggctgg gcggcgatga agcaaacgcc caggcgcaag
    10321 ggattctgga acgcgcggcg aagcgtgccg gattcaggga cgtggtattc cagtacgagc
    10381 cggtcgcggc tgggctggat tacgaagcca ccttgcagga agaaaaacgg gtgctggtgg
    10441 tggatatcgg cggtggtacg actgactgtt cattgctgct gatggggccg cagtggcgtt
    10501 cgcgtctcga tcgtgaagcc agcctgctgg gtcacagtgg ttgccgtatt ggcggtaacg
    10561 atctggacat cgcactggcg tttaaaaacc tgatgccgtt actgggcatg ggtggcgaaa
    10621 ccgaaaaagg catcgccctg ccgattctgc cgtggtggaa tgcggttgcc atcaacgacg
    10681 tacctgcgca gagtgatttc tacagtagtg ccaacggtcg tctgcttaac gatctggtac
    10741 gcgatgcccg cgaaccggaa aaagtggccc tgctacagaa agtctggcgt gagcgtttaa
    10801 gctatcgcct ggtgcgtagc gcagaagagt gcaaaattgc tctttcaagc gtagcggaaa
    10861 cccgcgcctc actgccgttt atcagcgatg aactggctac gctgattagc cagcaagggc
    10921 tggaaagcgc cctcagccag ccactggcgc ggattctgga acaggtgcaa ctggccctgg
    10981 ataacgccca ggaaaaaccg gacgttatct acctgaccgg cggtagcgcc cgttcgccgc
    11041 tgattaaaaa agcgctggca gaacagttgc cgggaattcc gattgcaggc ggcgatgact
    11101 ttggctccgt caccgccggg ctggcacgct gggcggaagt ggtgtttcgt taaaagaaaa
    11161 tggctcagca gcggcttaaa tctgcctacg cctgagccag tttttctgcc gcctgacgta
    11221 aaggttccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg
    11281 tcgcgccgac agtcaacgtc gattccagcc gatgtcgttt ggcggtcatt ttcgccatta
    11341 ccgcttgtct gtctgctggc gttatcgctt cattcggcct gaacacaaaa cggcgttcac
    11401 agctcgcttt ccagtcgata accgcctggg tcagatggtc gccaaaccct ttcacttgct
    11461 taacgccacg acgggtaaca tctgcggctg tttcaatacc aaaagaccgt aacgccgctt
    11521 tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc tccagaaact
    11581 tctgcttctg ccgttccctt gcagtgtcat gaagtgctgc cagagcgcgt ttctcttctt
    11641 ccggtaaccc gagaatttcg tccttcattt tttcgagcat cgcccgtttg gcgataaaac
    11701 cttccagccc gcccaactgt tggatctggc tgactaaatg ctcatagtcc attttcgcgc
    11761 gattaaatgc ctcacggcgt tgctggattt ctgctttgta cgctttgctt gtcagactgc
    11821 caataatcca gatagccgac agcacaggaa ccaaaataat ataacgcggt tctgcctgaa
    11881 ggccgcaaag caacagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcgc
    11941 gccgtaacag gcctaaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggca
    12001 attgcaatgc tggcggtgct actgacgcca tcaccatcgc ccagactttc gccagcacaa
    12061 aatcaccgcc ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat
    12121 tatccagcgc acaccacggg cagtcggtca aatgagcggg ataaacatgc attgccgaaa
    12181 cgggacattt ctttaactgt tggcgcagag aatcaagtgc cgctacccac gctttagccg
    12241 tcggacgccc ggttgccacg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat
    12301 cgcccggtaa catcgatagc ggaatcgatc gtggcggcgg ttttaaacca cgtcgctgat
    12361 tatctgacgc gtaggcataa cggaaatggg taatatccgt ctccagcgca ttacccgcat
    12421 cagagataag cggcacacca gaataaggat gccggccacc aaacaagacg tgaaaaatca
    12481 gcaacgcaag gccaaaatta tcgtgatttt ctgtgcgttc aaagccgaca aatgacgaca
    12541 aggtttgtag ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtgc
    12601 cattggcgtt aatctgaaag gagtcgctat caatcagtac cactttgctg tcgcgaccta
    12661 ccataaaact gttctggttt acgtcaccca cgacgtgccc gtgctcgtga accgtagcaa
    12721 aagatgaagc aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgagggtaac
    12781 gttgacgacg atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg
    12841 gcatcataaa accgataact tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg
    12901 cgacataatt caacaattgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt
    12961 ttaaggcggg tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcattgatat
    13021 cataaaccgc gccttcaccg cctttgccca gttcacggcc tggcgtaacg cactcaccag
    13081 tagcagtata taaagtgggt ttcatggtaa ccacagcgcc agtgccaacg ttttatcatc
    13141 atccgtacgc tcgtttaccg ccgggctgga caaaaactgc tttaacaatt caggtaataa
    13201 atcgagttgt tcctgcgttg ctgacgccag tccattgaaa aacggggtaa agaaaggtac
    13261 atgaggagaa ttatccagca tatttaacgc cagtcgctgg ataccatccg taaatgctgc
    13321 aactttatgc acacgctcag tgctggtgaa agtttccagt ctggaaacgg catcttcatc
    13381 ggtaatgaag tgcgtcatat tggcatattc acccaccatc ggcgtaagcg gcaattgcag
    13441 accgtgacca aaatccacca ccacgccgcc atcgccaatc tgcataatca aggtgccatt
    13501 agccgatgaa atcaatccca gaaacgtgca ggcaaaatcg cgaactgcta attctttggc
    13561 ttccgcctcg gcaaaaagtc gctgacgaac ggtcagcaca atatccgtgg cgaggatatc
    13621 attaagcccc aattccccac cctgcacttt ttgcgacata taagccatcg cttcattgac
    13681 ggcgagcgtc gcgccttctc ctccctgcga aacgctacct gcgccatcag caaggaacat
    13741 caccagtaat ggctgctggt catttagcca ggcaacctgc atctggcagg catcctggca
    13801 aggcaagtcc gcgctgatgt gcgatgttcc aacagcggat gcataaacca gacgccagct
    13861 cacactgacg tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg
    13921 gaaaccgaac gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccttgc
    13981 agaggcaaag gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca
    14041 ccgatggaaa agaaagcaaa ccttttatct tcttcccccc gaaacacttt gttggctgca
    14101 gcctgccact catcggttgg tgcaccatcg gtaatcagga aaatccacgg gcgataatac
    14161 gaaataccat ttgcacgata ctcacgtttc cgctcctcta ccatatccag ggctttggta
    14221 atggccgcac ccattggtgt atcgccctgg gcaaacagga tgggcggaaa aaaattagcg
    14281 gcgctggtaa agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact
    14341 ctttttaaag ccagcggatc ggcaagcagt tcatcacgaa aggtcaccaa tccggcgtta
    14401 agttcattaa taggtctgcc gctcatcgag ccagaaacat ccagcagcaa aatgcagggg
    14461 caacgtggtt ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata
    14521 agaaatcctt tcttagagaa ttatcgaggt ccggtatcat accgactgca ataatgaaat
    14581 aagagcaaac gaagaaactt tcagaattag gcccggcggc gcagaaaatt ttgctattcg
    14641 cctcgcaaga acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag
    14701 tgagggttag ggagaggttt ccccctcccc ctggtgttct tagtaagcct ggaagctaat
    14761 cactaagagt atcaccagta tgatgacgtg cttcatcata accctttcct tattaaaagc
    14821 cctcttctcc gggagaggct ttcccgtttc agcgtcccgc tgaaatcatc ggcttacctc
    14881 ctttcgccct gaatgcagtc tatcgctaac gcgtgaataa aatagtttcc tctgtgatta
    14941 ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg cagactacaa agcctgcgga
    15001 ttgacaatct tatcgtgaag gcatactttc aggagtgagg gtagagcggg gtttcccccg
    15061 ccctggtagt cttagtaagc ggggaagctt atgactaaga gcaccacgat gatgagtagc
    15121 ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt cccgtttcag
    15181 cgtcccgctg aaatcgtcgg cttacctcct ttcgccatgc acttagtcta tcgctgactt
    15241 gcagataaaa tagtttccag tgtgattatt gcgtaacgtc tcgcaaagaa tttttgctgg
    15301 caggatcgca gacggcaaag cctgcggatt gacaatctta tcgtgaaggc atactttcag
    15361 gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtatgctg ggtagctaat
    15421 cactaagagc accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc
    15481 ttcctcggga ggggctttcc cgtttcagca tccggctgaa atctgcggct tacctccttt
    15541 cgccctgtat gcagtctatc gctaacgcgt agataaaata gtttcctgtg ttattactgg
    15601 atgcgtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt cataattcct
    15661 ccatttttct cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag
    15721 agaaactctt aacgatgaaa ggcagttata aatcccgttg ggtaatcgta atcgtggtgg
    15781 ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc cggagtgcag
    15841 ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt
    15901 ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc
    15961 tcaccgggct tggcaccatt atcgccgcta atactgttac ggtgcgcagc cgcgtggacg
    16021 gtcaactgat ggcgttacat ttccaggaag gccagcaggt caaagcaggc gatttactgg
    16081 cagaaattga ccccagccag ttcaaagttg cattagcaca aacccagggc caactggcaa
    16141 aagataaagc cacgcttgct aacgcccgcc gtgatctggc gcgttatcag caactggcaa
    16201 aaaccaatct cgtctcccgt caggagctgg atgcccaaca ggcgctggtc agtgaaaccg
    16261 aaggcaccat taaggctgat gaagcaagcg tcgccagcgc gcagttgcaa ctcgactgga
    16321 gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc
    16381 aaatctccag tggtgatacc accggaattg tggtgatcac ccagacgcat cctatcgatt
    16441 tagtctttac cctgccggaa agcgatatcg ctactgtagt gcaggcgcaa aaagccggaa
    16501 aaccgctggt ggtagaagcc tgggatcgca ccaattcgaa gaaattaagt gaaggcacgc
    16561 tgttaagcct cgataaccaa atcgatgcca caaccggtac gattaaagtg aaagcacgct
    16621 ttaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc atgttagtcg
    16681 acaccgaaca aaacgccgta gtgatcccaa cagccgccct gcaaatgggc aatgaaggcc
    16741 attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg acgccgggca
    16801 ttcaggacag tcagaaagtg gtgatccgtg caggtatttc tgcgggcgat cgcgtggtga
    16861 cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtggtggaa gcccagagcg
    16921 ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct
    16981 gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc
    17041 tgtctccacc acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc
    17101 cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta
    17161 cccaggtgcc agcccggatg tcatgacctc tgccgttacc gcgccgctcg aacgccagtt
    17221 cgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat
    17281 cactttgcag ttccagctaa cattaccgct cgatgtcgcc gagcaggaag tgcaggccgc
    17341 gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa
    17401 agtgaacccg gcagatccgc cgatcatgac gctcgccgtc acctcaaccg ccatgccgat
    17461 gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg
    17521 cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca aacttaacgc
    17581 tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc
    17641 taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgtgcggtca cgctttccgc
    17701 gaacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct accagaacgg
    17761 cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa acagctggct
    17821 cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc
    17881 taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca ctgagagtct
    17941 gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc accaatatcc gcgcatccgt
    18001 cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct
    18061 gtttttgcgc aatattccgg cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat
    18121 cggcactttc gcggttatgg tgtttctcga tttttcaatc aataacctga cactgatggc
    18181 gttaactatc gccaccggat tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc
    18241 ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat
    18301 cggctttacc attatctcgc tgaccttctc actgattgcg gtgttgatcc cactgctgtt
    18361 tatgggcgat atcgtcgggc gactgttccg cgaatttgct attacgctgg cggtagcgat
    18421 tttgatctca gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag
    18481 ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag
    18541 gataatcgcc gcctatggtc gtggactggc gaaggtgctg aatcatccgt ggctgacctt
    18601 aagcgtggcg ctcagcacgc tgctgcttag cgttctctta tgggtgttca ttccgaaagg
    18661 tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac cgcaatccag
    18721 ctcctttgcc aatatggccc agcgacaacg ccaggtcgcg gacgtgattt tgcaggatcc
    18781 ggcagtgcaa agcctgacct catttgttgg cgttgatggc actaacccgt cgctgaatag
    18841 tgcacgttta caaatcaacc tcaaaccgct ggatgaacgt gatgaccgag tgcaaaaagt
    18901 cattgcccgt ctgcaaacgg cggtggataa agtgccgggc gtcgatctct tcctgcaacc
    18961 aacgcaggat ctgactattg atactcaggt cagccgcacc cagtaccagt ttaccttgca
    19021 ggccacctca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca
    19081 actgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt
    19141 caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggatg tcgataacgc
    19201 cctgtacaac gcgtttggtc agcgactgat ttccactatt tatactcagg ctaaccagta
    19261 tcgcgtagtg ctggagcaca acaccgaaat taccccgggc ctcgcggcgc tggataccat
    19321 tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc tcaattgcca aagttgaaca
    19381 gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt
    19441 taacgtgccg gataactatt cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa
    19501 gacgctgaat ctgccggtgg atatcaccac gcaatttcag ggcagcaccc tcgccttcca
    19561 gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt
    19621 gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac
    19681 cgcaggggtc ggcgcgctgc tggcattaat gattgccggt agcgaactgg atgtgattgc
    19741 gattatcggc attattttgc tgatcggtat tgtgaagaag aacgccatca tgatgatcga
    19801 cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa tctaccaggc
    19861 ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc ttggcgcgct
    19921 gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat
    19981 ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg tgatttatct
    20041 gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg aagaggaggc
    20101 gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt tactgtcggt
    20161 cgccattacc ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc cgctgccgca
    20221 ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac cagaaacaat
    20281 ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg cgcattgccg gagtcagtga
    20341 aatgacctcc agcagttcgc tcggcagcac gcgtattatt ttgcagtttg attttgaccg
    20401 ggatatcaac ggcgcggcgc gcgatgtgca ggcggcgatc aacgctgcac aaagtttgct
    20461 gcccagcggg atgcccagcc gtccgaccta tcgcaaagct aacccgtcgg atgcgccaat
    20521 tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg attttgcctc
    20581 gacgcagctg gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg atgttggcgg
    20641 cagctcgctg cccgccgtac gcgtcgggct gaatccgcag gcgctgttta atcagggcgt
    20701 ctcgctggac gacgtacgca ccgccatcag caatgccaac gtgcgtaaac cgcagggggc
    20761 gctggaagat ggcactcacc gctggcagat ccagaccaat gatgagctaa aaaccgccgc
    20821 cgaatatcag ccgttgatta ttcattacaa caacggcggc gcggttcgtc tgggcgatgt
    20881 ggcgacggtg accgactcgg tgcaggatgt gcgcaacgcc gggatgacca acgccaaacc
    20941 ggctattttg ctgatgatcc gcaagctgcc ggaagccaat attatccaga cggtagacag
    21001 catccgggca aaattaccgg agttgcagga aaccattccg gcggcgattg atctgcaaat
    21061 tgctcaggat cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat
    21121 tatctcggtg gcgctggtga ttctggtggt gtttttattc ctgcgctcgg gtcgcgccac
    21181 tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct
    21241 gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta ctggtttcgt
    21301 ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt catctggaag cggggatgaa
    21361 accgctacag gccgcgctac aaggtactcg cgaagtcggc tttacggtgc tatcgatgag
    21421 tctgtcactg gtggcggtgt tcctgccgct gctgttgatg ggcggattgc cgggccgact
    21481 gttacgcgaa tttgccgtga cgctttctgt cgccattgtt atatcgttgc tggtttctct
    21541 gacattaacg ccaatgatgt gtggctggat gctgaaagcc agcaagccgc gtgagcaaaa
    21601 gcgactgcgt ggttttggcc gcatgttggt agccctgcaa caaggctacg gcaagtcgct
    21661 gaaatgggtg ctcaatcata cccgtctggt gggtgtggtg ctgcttggca ccattgcgct
    21721 gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg acaccggcgt
    21781 gctgatgggc gggatacagg cggatcagag catttcgttt caggcgatgc gcggcaaatt
    21841 gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggctttac
    21901 cggcggttca cgagttaaca gtgggatgat gtttatcacc ctcaagccac gcgacgaacg
    21961 cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga aagaaccggg
    22021 ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc agtcgaacgc
    22081 cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat gggagccgaa
    22141 aatccgcaaa aaactggcga cgttgccgga actggcggac gtgaactccg atcagcagga
    22201 taacggcgcg gagatgaatc tggtttacga ccgtgacacc atggcacggc tgggaatcga
    22261 cgtacaagcc gccaacagtc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat
    22321 ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct atacccagga
    22381 catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga tcccgctgtc
    22441 gtatttcgct aaatggcaac cggcgaatgc cccactatcg gtgaatcatc agggattatc
    22501 ggcggcctcg accatttcgt tcaacctgcc gaccggaaaa tcgctctcgg acgccagtgc
    22561 ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg gcagttttgc
    22621 cggaacagcg caggtgttcc aggagacgat gaactcacag gtgatcctga ttatcgccgc
    22681 catcgccacg gtgtatatcg tgctgggtat cctttacgag agttatgtgc acccgctgac
    22741 gattctctcc accctgccct cggcgggtgt tggagcgctg ctggcgctgg agctgttcaa
    22801 tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca tcgtgaagaa
    22861 aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc
    22921 gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga tgactaccct
    22981 ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc ggcgacggct cggagctgcg
    23041 gcaacccctg gggatcacca ttgtcggcgg actggtaatg agccagctcc ttacgctgta
    23101 taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaacc
    23161 taaacaaacg gtaaccgagt aaatgacaga tcttcccgac agcacccgtt ggcaattgtg
    23221 gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtaa acaccgccct
    23281 tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc
    23341 ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg
    23401 cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac tgttttgcgc
    23461 gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggcgc
    23521 gatgatggtg ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg agcaatatat
    23581 ggcggcgatg acctttgtca cgttacccgg tcaggtcggt ccgctgctcg gtccggcgct
    23641 cggcggtctg ctggtggagt acgcgtcgtg gcactggatc tttttgatca acattccggt
    23701 ggggattatc ggtgcgatcg ccacattgat gttaatgccg aactacacca tgcagacgcg
    23761 gcgctttgat ctttccggct ttttattgct ggcggttggc atggcggtat taaccctggc
    23821 gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc tggtcgcagt
    23881 tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc gcgccctgtt
    23941 cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga gctttgccgg
    24001 acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa ttggcctcgg
    24061 tttctcgccg tttcatgccg gactgatgat gatcccgatg gtgcttggca gcatgggaat
    24121 gaagcgaatt gtggttcagg tggtgaatcg ctttggttat cgtcgggtac tggtagcgac
    24181 cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgctctgt tgggctggta
    24241 ctacgttttg ccgttcgtcc tgtttttaca aggaatggtc aactcaacgc gtttctcctc
    24301 catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg gaaacagcct
    24361 gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc accatcgccg ggctgttgct
    24421 gggacttttt ggttcgcagc atgtcagcgt cgacagcggc accacacaaa ccgtctttat
    24481 gtacacctgg cttagcatgg cgttgatcat cgcccttccg gcgttcatct ttgccagagt
    24541 gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg cgcaatgaag
    24601 ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac ctgcattgtc
    24661 ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt tattgattac
    24721 atcaagcatg gtaatgaaca acgattacaa ctgttaagtg atgcgcttgg cgagcagtat
    24781 gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc gctttgtctt tcagatcctg
    24841 cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc accgcacggc
    24901 tggcgtaccc agttctgggt ggttgatcaa aacaacaaag tgctggttgg tccgcgagcg
    24961 ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga agttggcgcg
    25021 gtgatcgcct cccccgttga gcggttaacg cgcaatactg atatcaattt cgataaacaa
    25081 cagcggcaaa ccagctggct gatcgtcgcc ctggcaacgt tactcgcggc gctcgccacc
    25141 tttctgctgg cgcgcggttt gctggcaccg gtaaaacgac ttgtcgatgg cacgcacaaa
    25201 ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga actgggcaaa
    25261 ctggcgcaag acttcaacca gcttgccagc acgctggaga aaaaccagca aatgcggcgc
    25321 gattttatgg ccgatatttc acacgaactg cgtacgccat tagcggtgct gcgcggtgaa
    25381 ctggaagcca ttcaagatgg cgtgcgcaaa ttcacgccgg agacggtggc gtctttacag
    25441 gcggaagtcg gtacactgac taaactggtt gacgatcttc atcagttgtc gatgtctgat
    25501 gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct ggaagtggcg
    25561 ggcggcgcat ttcgcgaacg atttgccagc cgtggcctga aactgcagtt ttccctgcca
    25621 gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa taacttactc
    25681 gaaaacagcc tgcgctacac tgatagcggc ggcagcctga aaatctctgc cgagcagcac
    25741 gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga cgagcagcta
    25801 caaaaattat ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc cagcggtggt
    25861 tccgggctgg ggctggcgat ttgcctgaac attgttgaag cacataatgg ccgcattatt
    25921 gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct ggaacgggat
    25981 ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt attttgatcg
    26041 tggaagatga accgaagctg gggcagttgc tcattgatta tctgcgtgct gcgagctatg
    26101 cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag acaccaccgg
    26161 atctgatcct gttagatctg atgctccctg gcaccgatgg cctgacgctg tgccgggaaa
    26221 ttcgtcgttt ttctgacatt ccgatcgtga tggtgacggc aaaaatcgaa gagatcgatc
    26281 gcctgctggg gctggagatt ggcgcagatg attatatctg taagccgtac agcccacggg
    26341 aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa accgcagcgc gagttgcagc
    26401 aacaggatgc tgaaagcccg ttgattatcg acgaaggtcg ttttcaggct tcatggcgcg
    26461 gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct gaaaacgctc tctcatgaac
    26521 caggaaaagt gttctcccgc gagcatttgc tcaatcatct ttatgacgac taccgcgtag
    26581 taaccgaccg caccatcgac agccacatta aaaacctgcg ccgcaagctg gaatctctcg
    26641 acgccgaaca gtcatttatc cgcgccgttt acggcgttgg ttaccgctgg gaagccgacg
    26701 cctgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg cgttcagacc
    26761 agatccggca acattatccc acgcatggtc agcaaactga cattatctct ccgccctggc
    26821 aggcatcaga aaatcgctca tactttaatc ggtaaacagc acctttagat gctgttttcg
    26881 atacgcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag taagagcagt
    26941 gataatcagt tccggtttgt gctaaaagcg ggcaatggtg agactatcct caccagcgag
    27001 ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag caacagcccg
    27061 caagaagaac gctatgagaa aaaaacggca agtaacggca aattctattt caatctgaaa
    27121 gccgctaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca atctcgtgaa
    27181 accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga caatacgtaa
    27241 ttacgatgcc gggcgtgggt tgttggcgtc cggctttata tccaaataac gcttagttat
    27301 taataattaa gagtaaccgc cgccattaaa atagtgggca ctgacgagca ctcgttgtgc
    27361 tttctttgtt taccatccca tacttatcct cctgaacatg ttcatgtgcc aaaatatctt
    27421 ctaatacttt cgtgacgcta tccggtttat gacatacact gatgtaagat ttttacttac
    27481 aggcgggcgt aaaatggcca gaacgatgac ggttgatctc ggcgatgagt tgcgtgaatt
    27541 tatcgagtcc ttaattgaat ccggcgacta tcgcacccaa agcgaagtga tccgtgagtc
    27601 actgcgatta cttcgagaaa agcaagcaga gtctcgcctg caagcgttac gtgacatgct
    27661 cgcagaaggg ttgagtagtg gtgaagctca gccgtgggaa aaggacgcgt tcttacggaa
    27721 ggtgaaagca gggataagga aataatgtgc gtattattaa actcatgcca aaagccaatg
    27781 aggatttaga aggcatctgg tattacagct atcaccattt cggcgaacca caagccgaca
    27841 gatatgtgga acatctttca gacgttctcc agattctgag taacaataat ataggtacgc
    27901 ccagacctga attaggcgaa ggtatttttg ttcttccttt tgaacgccac gtcatttatt
    27961 tcctgcaatc accaggggaa attatcgtca ttcgtatcct taaccaaaac caggacgcca
    28021 ccagacattt acactggagc taaacccgcc gattgcgcta caatgcccgc ccttaaagtg
    28081 ggggcactcc cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga
    28141 gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg
    28201 ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttattcc ctgcgtgtgc
    28261 gcaacaacga attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg
    28321 ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta
    28381 tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag
    28441 ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc gatccacctt tcggtgcagg
    28501 ctaacgccgt gaactgggcg acggtgaaat tctggcagca aatgggcctg acccgcgtga
    28561 tcctctctcg cgagctgtcg ctggaagaga ttgaagagat ccgcaatcag gtgccggata
    28621 tggagatcga gatcttcgtt cacggcgcgc tgtgcatggc ctactccggt cgctgcctgc
    28681 tctctggcta tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct
    28741 gggagtacaa cgtccaggaa gggaaagaag atgatgttgg caacatcgta cacaagtacg
    28801 agccgattcc ggtgcaaaat gttgagccga cgctgggtat cggcgcacca accgacaaag
    28861 tgtttatgat cgaagaggcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc
    28921 acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga
    28981 ccaaaatggg cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgtg
    29041 cacgcaccgc acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg
    29101 ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc
    29161 tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca gtttctgacc
    29221 gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgcg gcggtagcgg
    29281 tgaaaaataa attctccgtt ggcgacagcc ttgagctgat gacgccgcaa ggcaacatta
    29341 attttaccct tgagcacatg gaaaacgcca aaggcgaagc tatgccgata gcaccaggcg
    29401 atggttatac tgtgtggctc ccggtcccgc aggatcttga gctcaattac gcgctgctga
    29461 tgcgtaattt ctccggggaa accacgcgta atccccacgg taagtgatta atttcgatta
    29521 tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta
    29581 tcatccgttt cgctgaaaaa cataacccat aaaatgctag ctgtaccagg aaccacctcc
    29641 ttagcctgtg taatctccct tacacgggct tattttttat gcataagccc tatccctggt
    29701 caccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg cctttcacgt
    29761 aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca tgtttgacgc
    29821 ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg ccatgtaatc
    29881 ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta acacagaaat
    29941 gggctgcatc attttttact atattttctg caccagatat tctgcccctg gctaaagaag
    30001 cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt ttatatgcaa
    30061 gaccaaattt accgatactt gtctcatcat tatgcacagc acaaccagac ataataaaaa
    30121 tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt aaata
//
LOCUS       DAZFDP010000043        29619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFDP010000043 DAZFDP010000000
VERSION     DAZFDP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHJ1G_002030"
     CDS             complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHJ1G_002030"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4763888.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(805..1353)
                     /locus_tag="ACHJ1G_002031"
     CDS             complement(805..1353)
                     /locus_tag="ACHJ1G_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4763889.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHJ1G_002032"
     CDS             complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHJ1G_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4763890.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHJ1G_002033"
     CDS             complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHJ1G_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4763891.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTNCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHJ1G_002034"
     CDS             complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHJ1G_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4763892.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHJ1G_002035"
     CDS             complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHJ1G_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4763893.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSRTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHJ1G_002036"
     CDS             complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHJ1G_002036"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4763894.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11027..11806
                     /gene="elyC"
                     /locus_tag="ACHJ1G_002037"
     CDS             11027..11806
                     /gene="elyC"
                     /locus_tag="ACHJ1G_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4763895.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11783..12676)
                     /locus_tag="ACHJ1G_002038"
     CDS             complement(11783..12676)
                     /locus_tag="ACHJ1G_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4763896.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHJ1G_002039"
     CDS             complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHJ1G_002039"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4763897.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHJ1G_002040"
     CDS             complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHJ1G_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4763898.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHJ1G_002041"
     CDS             complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHJ1G_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO4763899.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHJ1G_002042"
     CDS             complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHJ1G_002042"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4763900.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHJ1G_002043"
     CDS             complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHJ1G_002043"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4763901.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17844..20108)
                     /locus_tag="ACHJ1G_002044"
     CDS             complement(17844..20108)
                     /locus_tag="ACHJ1G_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO4763902.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHJ1G_002045"
     CDS             complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHJ1G_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4763903.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHJ1G_002046"
     CDS             complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHJ1G_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4763904.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHJ1G_002047"
     CDS             complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHJ1G_002047"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4763905.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHJ1G_002048"
     CDS             complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHJ1G_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4763906.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHJ1G_002049"
     CDS             complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHJ1G_002049"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4763907.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHJ1G_002050"
     CDS             complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHJ1G_002050"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4763908.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26973..27665)
                     /locus_tag="ACHJ1G_002051"
     CDS             complement(26973..27665)
                     /locus_tag="ACHJ1G_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4763909.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHJ1G_002052"
     CDS             27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHJ1G_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4763910.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7192 a         8055 c         7190 g         7182 t
ORIGIN      
        1 gtcccctgca atattttgaa tttgcacgat tttgtaggcc ggataaggcg ctcgtgccgc
       61 atccggcata aacaaagcgc actttgccag aaatccaggc ccgcattaaa agcgggcttt
      121 tttatgtgcc ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat
      181 aaccaagtgt ggataatggt ccgtgacccg gaataaatgt cacgtcatcc cccagtggca
      241 gcaatttatc tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc
      301 gccctactcc gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa
      361 aaaacacgac atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc
      421 ctatgctgat ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt
      481 ccagaccaaa catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt
      541 ccgggccaaa aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt
      601 ccagatgacc atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct
      661 gtttgatttt ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac
      721 accagattaa tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac
      781 tgcccctgtt tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac
      841 aaagttacta cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg
      901 aatattgctt aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata
      961 gcttttcttc gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg
     1021 ataaccggaa atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa
     1081 ctggtcgaat aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa
     1141 atggttaagt tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt
     1201 gattgactct ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag
     1261 tgttgcaaac gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag
     1321 caatttgcgg cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta
     1381 tcgtttatat gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca
     1441 agatgactca accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt
     1501 attacgacaa ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga
     1561 tacgatttgc gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg
     1621 ggtacgacca tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg
     1681 ccgaatattg acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt
     1741 ccagcctgca tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca
     1801 gcctgagctc aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa
     1861 ataaatggcc tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc
     1921 tggagcctgc tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg
     1981 ccatgggtca atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc
     2041 gagatatccc ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg
     2101 tacgttccac ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt
     2161 gcgatcgggg cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta
     2221 gaccagcgaa taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa
     2281 gcgcaatcgc tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa
     2341 ccagtcacgt gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa
     2401 acgtttaacg gcttccacca gttcattatc gtaggcggca cgaacggcag gcgcaggttt
     2461 actacgagac gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc
     2521 ggatgggctg actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc
     2581 cccgtccaac atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca
     2641 ctgccctggg cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag
     2701 taaggccagt aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtcctgc
     2761 aacaaacgta ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag
     2821 cggcggcgtt gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg
     2881 aatatttgca atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc
     2941 catcccgtta acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa
     3001 accggcaatc gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc
     3061 ccacatgggt tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg
     3121 cttgtaacct gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc
     3181 aggcaatggc tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc
     3241 ctgttcactg acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc
     3301 attgaacagt ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc
     3361 acacatcata ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg
     3421 ctgttatttt acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc
     3481 tgttactcat caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat
     3541 gtaaagaagt ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc ggaaacggag
     3601 ttttcggaaa aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc
     3661 gcttcgtcac ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg
     3721 acgtgaacgt gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc
     3781 tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg ctcacacaat
     3841 tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg
     3901 cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc
     3961 accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac
     4021 tctgcgcgca accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg
     4081 cggtaatcca gcagttcttc gccaatggtc tgcggcgtgc gctgtcccat atcaatctgc
     4141 ggattaagac gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta
     4201 aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt
     4261 tcacgcacgt tcacgttgag gcgcacgctg ttcacctgac caaacgatac gttctgcaac
     4321 ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc
     4381 gccacgctgc gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga
     4441 cggctaagtt cgatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata
     4501 tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc
     4561 tccggacgtt tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc
     4621 accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag
     4681 agataagcca gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc
     4741 atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc
     4801 cgctccagct tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc
     4861 gctttttcca gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca
     4921 cggcgaatac gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat
     4981 tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc
     5041 agcacctggt tgtactgact caactgcgct gcgtgaccac gcaacgcttc acgagcgcga
     5101 gtacgctccg cttccgcctg ttccagacgt tcacgcagct tttcgttgag atcgctatta
     5161 ccgctcagca tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc
     5221 gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct
     5281 tcttttagct gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt
     5341 ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga
     5401 cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc
     5461 ggcagaatgc ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc
     5521 tgctggttat cattttcatg attactcaac gcccgctcca gttcgacgcg acggctgttc
     5581 agttgacgga tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata
     5641 aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg
     5701 aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca
     5761 gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt
     5821 ttcaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc
     5881 ggatctcctt cgatcagata gagatcttcc gggcaattgg tcaagccttc caggtgttca
     5941 gttatctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag
     6001 aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca
     6061 aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt
     6121 tcgatctctt catcgacggc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc
     6181 tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac
     6241 tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag ccaaaccggc
     6301 gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgttcctg gcgcagtgcc
     6361 atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg cgcttccagc
     6421 tcctgatgca gagcttccag ctcgtcaatg tcaaaattct tgccctgacg tttgcagaaa
     6481 tctgccagca gacgctcagc ttcttgctgt tcgcgcagac gctgttccag ttcgcttaag
     6541 cgcatccgca acggctgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcaat
     6601 agttcgcgag cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc
     6661 agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca
     6721 agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg
     6781 gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca
     6841 gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag
     6901 ttagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctcg
     6961 ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc
     7021 tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt
     7081 tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca
     7141 ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt
     7201 tgctgacgcg aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag atggacacgg
     7261 cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga
     7321 tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt
     7381 tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac
     7441 aggtagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg
     7501 atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc
     7561 agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct
     7621 tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg
     7681 ttcagggttt cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca
     7741 aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc
     7801 accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc
     7861 agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta
     7921 cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catggtggtg
     7981 gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt
     8041 cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc
     8101 attattcttc ctctccgcta tctggctgat tttcttcggt ttcatcgttg agttgcagat
     8161 gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat
     8221 cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cgaaacttgc
     8281 tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac
     8341 gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca
     8401 gtttcagcag ttttgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg
     8461 tgaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc
     8521 cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc
     8581 gtaaatagaa gaacccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact
     8641 cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa
     8701 tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct
     8761 gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg
     8821 gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct
     8881 acgccaagac gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc
     8941 ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt
     9001 ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac
     9061 tcttcgtatt ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc
     9121 tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca
     9181 tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata
     9241 tcgatcgcgg tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg
     9301 gattgctggc cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga
     9361 tcgacgaaat gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc
     9421 tgcaatttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt cctggaagtt
     9481 tccgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg
     9541 gcgatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata
     9601 ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga
     9661 aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc
     9721 gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta
     9781 atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta
     9841 aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg
     9901 atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg
     9961 ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa
    10021 gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt
    10081 tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg
    10141 tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta
    10201 attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa
    10261 acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa
    10321 cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc
    10381 ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat
    10441 tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata
    10501 cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg
    10561 tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca
    10621 caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata
    10681 aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgccag
    10741 catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc
    10801 acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt
    10861 ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt
    10921 ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata
    10981 tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact
    11041 gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc
    11101 tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat
    11161 tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc
    11221 catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct
    11281 cggagatggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag
    11341 tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat
    11401 cttcacggga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt
    11461 tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac
    11521 cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac
    11581 ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct
    11641 accagccccg gcgaatcagt tggccatcga ctcgccgctt aacccgtggg aacgggcaat
    11701 cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat
    11761 ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat
    11821 cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca
    11881 catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc
    11941 agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg
    12001 cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca
    12061 gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa
    12121 ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac
    12181 cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc aggtaccatg
    12241 agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac
    12301 ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg
    12361 ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc
    12421 cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc
    12481 ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc
    12541 ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg
    12601 ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg
    12661 cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca
    12721 atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt
    12781 aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt
    12841 cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag
    12901 caacagcaac atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga
    12961 tgtgttctaa cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc
    13021 cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac
    13081 gatcacgatc ccaaggaatg gtggcgcgtg agaagtacag tgcataccct tcagcgtcga
    13141 gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt ggcaccgcca
    13201 gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg
    13261 cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc
    13321 atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac
    13381 atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg gttgccacga
    13441 tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc
    13501 cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa
    13561 tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag
    13621 caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc
    13681 ttgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag
    13741 caaacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat
    13801 aatcatttat gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg
    13861 cgggcagcgt cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc
    13921 ctgacgtaac cctttttgca gcattgtcgt tggtttaatg ccttcctgta accacagaga
    13981 gataacttca aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg
    14041 cccacgatgt aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata
    14101 gcactccagc cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat cccagacaac
    14161 aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg ccagcgcacg
    14221 ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac
    14281 agcaatgatt tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt
    14341 cagccgatag taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt
    14401 atccagcatg atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc
    14461 aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat
    14521 cacctttccg gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca
    14581 gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ccttatcatg
    14641 aatatgctga attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta
    14701 tttccagccc attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg
    14761 cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc
    14821 atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat atggactacg
    14881 ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat
    14941 atcctccaac gacgcttgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag
    15001 attacgcgca tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac
    15061 gctgccgcgg cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc
    15121 tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa
    15181 ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa
    15241 cgcactgaca tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg
    15301 ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat
    15361 cgccactaca tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc
    15421 ctgacccggc agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat
    15481 taccgcatca accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca
    15541 ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag
    15601 acggtaatgc tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag
    15661 aatggctttt accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtgcgttg
    15721 ataaatcaac acaggttcat cacccgcctg tgctgttgtg gtatctgccg acaataacag
    15781 cggataagat tcagccttac caccatatcc ccgcgatacg acccccacgc gaataccgcg
    15841 ctgttgcaac tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcggt
    15901 gagattacca accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt
    15961 atagcaaaga cggatcgcgc cacttaccag gccatacaac caggagagtg gcagcaatag
    16021 ccgccacaaa ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg
    16081 tgaagttgcg cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc gcgttccaca
    16141 atgacaccat cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg
    16201 gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt
    16261 tcggattcgg tatccagagc cgaggtagct tcatccagaa tcagaatcgg gctatcacgc
    16321 agcaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt
    16381 tcaccaatca ctgtatcgag accgttatcc atcttattga tgaagtccat ggcgtaggcc
    16441 atacgcgccg cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc gtaagcaatg
    16501 ttgttagcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta
    16561 cgtaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca
    16621 tcaatatcgt aaaaacgagt gatcaggctg gcgatggttg atttacctga accagagcgt
    16681 ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcaggt
    16741 acgtcacgtc ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc agtcgcacgc
    16801 tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtc
    16861 tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagtga tttcagcgga
    16921 cgcatcagtg caatcattga agagaaaaca acggtaatcg taccggcagt caggctatcc
    16981 atgacacttg ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc
    17041 tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt
    17101 cggttgctga ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact
    17161 tctttgtggc ccttcagcat ttgttcggcg ctggtggtca cctgccccat ggtgttctgc
    17221 atgtttttac tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat cgaaacaatc
    17281 ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac
    17341 aggccgatga ttgacgcacc ttcacgcaca accgttatca gtgcgccgga agaagaagaa
    17401 gcaacctgtt cggaatcgta ggtgatacgt gacaacagcg tccccgttga ctgtttgtca
    17461 aaaaatgaaa ccggcattcc catcatgtga ccaaacaggc ggcgacgcat ggtcattacc
    17521 acctttcctg atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa
    17581 atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca
    17641 aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg
    17701 aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat ggttggccac
    17761 agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc
    17821 atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga
    17881 taccaacgag gtaaaatttg ctctctgagg ctgctaatcc gccagccttg cgctgaaaaa
    17941 ttgaccgtca cttgaccctg atgtggagta tcaagccatt gatatccttg ctgttgatag
    18001 cgatgcttaa ctttattaga gggcaatcgc catgcgttat agcgcgatgc cgatgcgagt
    18061 gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg
    18121 tgaggtacct gaagcaatgt tgtctgcact tgctgccagt aacggcttag catcttttgt
    18181 tcagcgggga cttcaatatc accggttaga agaatgctat tcgtcccgtc atcaacctta
    18241 accacacagg aatggttatt tcctttatcg ttgctagctt gtaaaggcca gtgcacgctg
    18301 aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg atgttcccag
    18361 tttaacggac ttctgatcca taacatcggc catgtgtgca atattgagtc cagccctccc
    18421 cggtgatcca gatgttcatg actcagaata acgccttccg gttcaagatt atgccagtgg
    18481 agccagggga taatcagttg ttgcccacta tccccttcgg gccaggccag tcctgtgtca
    18541 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    18601 atgtacacct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    18661 cctacagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    18721 aagaaccatg gggaaaatga tagccattgc caacgttcag caatgttgat ccacccttct
    18781 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    18841 ccttgctcca ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag gatcaacgga
    18901 accgtgataa atgtcaccaa gggaattgct aatagatttg caataaacga ggtcagacta
    18961 atgccatgaa atatgacgat ttgcacaggc ataagcagga gtgtgattcc cagttgcaaa
    19021 tgaatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    19081 cactggtacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    19141 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    19201 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    19261 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattcca
    19321 ccaattaatg gcatttgcca gtggatccag cgcccaggca gataaacttg cccaccgcga
    19381 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    19441 tgcgccgttc cagtatcacg catgatattt ttgatttctt tggggacgga taaccgttcc
    19501 cccataccta aaccaagaat aaccgcattc cacatatagg gttgcagcgt tgtttgtagt
    19561 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaag
    19621 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    19681 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    19741 accgcttgcg gaagatattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    19801 ccttgtagat gagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    19861 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    19921 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    19981 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcccaggc
    20041 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    20101 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    20161 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    20221 tcaaaactct gcgaggcagc ttccagttcc tgattagaga aatgaaaaaa gcacccgaca
    20281 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    20341 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    20401 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    20461 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    20521 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    20581 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    20641 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    20701 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    20761 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    20821 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    20881 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    20941 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    21001 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    21061 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    21121 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    21181 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    21241 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    21301 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    21361 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    21421 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    21481 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccaggcct tcaacgcctt
    21541 cttcgatttc aacgaagcag ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg
    21601 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccaac tgtttcaggc
    21661 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    21721 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca agccatgtca gtgatgtgca
    21781 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    21841 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    21901 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    21961 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    22021 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    22081 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    22141 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    22201 cgtcttctac tgcgtccaga gcaacgtcaa cttcatcacc tacctggatt tccagctcgc
    22261 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    22321 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    22381 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    22441 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    22501 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    22561 ctggcgcgcg tattgtagcg ctttttcaat cacttgctca atgcttaagg tggtggaatc
    22621 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    22681 gtcgcgttct ttgatctcgg ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg
    22741 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    22801 tggcgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca ggccgggtaa
    22861 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    22921 ttgtgaagct gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    22981 gaggatcact tccagattgc cattggttga cacaaaacgt acatccagat gggatgccag
    23041 cggtaccagc gcatcttctg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    23101 tacgcgataa attgcacccg aatccagcag atgccattgc aatgcttccg ccatagcctt
    23161 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    23221 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    23281 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    23341 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    23401 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    23461 agtcaaacat ggactgggtg cgaccaccat ccagactagc cagtttttca aagctgccaa
    23521 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaggagaaa tcatctgctt
    23581 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    23641 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    23701 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    23761 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    23821 cacggacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    23881 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    23941 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    24001 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    24061 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    24121 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    24181 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    24241 caaaatgact cacaaggtcc gaaaaaaaac gccgacaatg tgccgacgtt ttgataaaga
    24301 aagatttggc tatttattgc ccgttgttca ttcaggctgc ctggctaatc cgcgccaact
    24361 gctcgaaata atccggaaat gttttggccg tacatttggg atcaagaatc gtcactggtg
    24421 tatctgacaa cgccaccagc gagaaacaca tcgccatccg gtgatcattg tacgtcgcga
    24481 tctcggcaaa gttcagtttt tccggtggag tgatacgaat gtaatcgtgc ccctcttcta
    24541 cttccgcacc gactttacgc agttctgttg ccatcgcaaa caggcgatcg gtttctttaa
    24601 cacgccagtt atagatattg cgcagcgtgg tggtgccttt tgcaaataac gccgccgtgg
    24661 caatggtcat cgccgcatcg ggaatatggt tcatatccat atcaatagcg ttcagttcac
    24721 cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt ttttccagca
    24781 catcagcaaa gcgaatatca ccctgcatac tgttacgtcc aataccggtc acttttacag
    24841 tgccgccttt gattgctgct gctgccagaa agtaagaagc cgaagatgca tcgccttcga
    24901 ccaaataagt acccggagac tgataagact gcccgccttt tacgacaaat tgttgatagt
    24961 gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagtgtgatg tcgatataag
    25021 gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca agaggcgcag
    25081 tcattaacag tgcggtgagg aattggctgg aaacggagcc atcaacgtca acgttgccgc
    25141 cagtaaagcc gccctgtaaa cgcaacggcg gataattttc ttgttccagg taagtgatct
    25201 tcgccccgcc caggcgcagc gcatccacca gatgaccaat cgggcgttct ttcatacgcg
    25261 gctcaccggt cagcacaata tcattgctac ccagacaaag agctgccgcc agcggacgca
    25321 ttgccgttcc ggcgttaccg aggaacaact ccagggcacc ttctgcgtgt aatggaccgc
    25381 cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt acccctaacg
    25441 ctgttaatgc attcagcata tggcgcacgt catcgctatc cagcagattg gttaatactg
    25501 ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt agaaacgctc ttggaaccgg
    25561 gcagattaat agtgccatcg acacgagcga tgggttgtaa cgtcagggat tccatgaact
    25621 caactctcta caacagaaat aaaaacccca caggctggct gtggggatta agcaaaattt
    25681 cggcattaac cgtgacggcg ttcgaactca accatgaagt ctgtcagcgc tttaacgcct
    25741 tccagcggca tggcgttata aatagaagcg cgcattccgc cgaccacacg gtgacctttc
    25801 agtgcatgaa ggccagcagc aaaagactct tcaaggaaca atttgtcaag cgcactgtcc
    25861 gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacgtc attgcggtag
    25921 aaatcgctgt tatcaatcac cccatatagc agttctgctt tttgctgatt gattttatcc
    25981 atttcagcta caccgccgtt cgctttcagc catttaaaga ccagaccaga tagataccag
    26041 gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt tgaggatgga ataatccaga
    26101 atcgacggac acgcgatatt cgctttgccc agcaaatctt cacgaacgat gacgattgtc
    26161 aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa tcacgccata acggctgacg
    26221 tcaatcggac gggaaagaat ggttgaagag aagtcggcgg cgaccaccac atctttgccg
    26281 aagtctggcg tttcgtcgat ggcgataccg tcgatggttt cattcgggca gtaatgcata
    26341 taagcagcat tatcagagag ttgccattca cgcattggct taaccgcgcg cagaccatca
    26401 acagtcactt tggcgtcaaa gacattaggc gtgcagtatt ttttcgcttc tttaatggca
    26461 cttgccgccc agtaaccggc atcaacataa tctgcggtgg ttttatcacc gagaatattc
    26521 agcggtaccg cagcaaactg accgcgacca ccgccatggc agaataatac cttgtagttg
    26581 gaggggacat taagaagatc gcgaaaatcc ttctcggctt cctctgcaac ctgaatgaac
    26641 tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca gtcgcgcagt
    26701 tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga actaaaattg
    26761 aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgctg tcaatcgatt
    26821 gcgaccacag gtaatacatt attcctgtgg ctatcggttt tatcattcag tgacacacat
    26881 cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc atcacacaaa agaatctctc
    26941 ggattattag caaaaagccg atgtttttac tgtcaggtca cccgttttgc ccgacttgcc
    27001 ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc tttcatatga
    27061 atgatttcac tacaacgaat acaggcatag tccgccgact ccggcaccac tttgtaacgc
    27121 tgcgtacaat cactgggtaa aataagtaaa cccggtttca ccttgtcatc ttcaaagaaa
    27181 tagacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc cagctgctcc
    27241 acgccacgca atcgtagctc cataaagaac tcaaattccg tcatgttgga gttattgagt
    27301 ttcgaccagg ccagctcgcc atcttcaata ataacaaccg gcttaccttc cagtagatct
    27361 tccagttttt cactgtgcgc catcaaccac attaccaggc ggtataacaa cgccagagtg
    27421 ataaagacga taagtaccgg caccatcggc acatcatcat aaaacgccac atctcccgcc
    27481 gctgaaccca gcgtgagaat gattaacact tcaaacagcg acatctgtcg cacaccgcgt
    27541 cttccggtca ttttgaggaa caaaaaaacc agtacaaagg tataaagact acgtagtgca
    27601 acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca tacggtggag atcgaacgcc
    27661 ttcattttta tgctcttatc gtggtttatc cgctaaaagc atagtcaaca gttcatcggc
    27721 atggcggcgg tgatagcgcc tttgagataa aacccgtatt attgcgcgct ttccgtacga
    27781 ctaaagtgat tttcatgacg caaacattta tccccggcaa agatgccgct ctggaagatt
    27841 ccatcgctcg cttccagcaa aaactttcag acctcggctt tcagattgaa gaggcctcct
    27901 ggctgaatcc tgtgcctaac gtctggtctg tacatattcg cgacaaagag tgcgcactgt
    27961 gttttaccaa cggaaaaggc gcaaccaaaa aagcggcgct ggcttctgca cttggtgaat
    28021 atttcgagcg tctctcaacc aactactttt ttgctgattt ctggctgggc gaaaccatcg
    28081 ccaacggtcc gttcgtgcat tatcccaacg aaaaatggtt cccactgacc gaaaatgacg
    28141 atgtgccaga aggactactc gatgaccgtc tgcgcgcgtt ttatgatccg gagaatgaac
    28201 tgaccggcag catgctgatt gacctacaat ccggtaacga agatcgtggt atttgcggcc
    28261 tgccgtttac gcgtcagtcc gacaatcaga ccgtttatat tccgatgaat atcattggta
    28321 acctgtacgt ctccaacggt atgtccgcag gtaatacccg caacgaagca cgcgttcagg
    28381 gattgtctga agttttcgaa cgctacgtga aaaaccgcat tattgctgaa agcatcagcc
    28441 tgccagagat cccggcagac gtgctggcgc gttacccagc agtagttgaa gccatcgaaa
    28501 cgctggaagc ggagggtttc ccaatcttcg cttatgacgg ttcgcttggc ggccagtatc
    28561 cggtgatttg cgtggtactg ttcaatcctg ctaacggtac ctgctttgcc tctttcggtg
    28621 cgcatcctga ttttggcgta gcactggaac gtaccgtgac cgagctgctg caaggtcgtg
    28681 gcctgaaaga tttggatgtg tttactccgc caaccttcga tgatgaagaa gtcgctgaac
    28741 ataccaacct cgaaacgcac tttatcgatt ccagcggttt aatctcctgg gacctgttca
    28801 agcaggatgc cgattatccg tttgtggact ggaatttctc cggcaccacg gaagaagagt
    28861 ttgctacgct gatggctatc ttcaacaaag aagataaaga agtttatatt gccgattacg
    28921 agcatctggg cgtttatgct tgccgtatta tcgtgcctgg catgtccgat atttatccgg
    28981 ctgaagatct gtggctcgcg aataacagta tgggcagcca tttacgtgaa acgattcttt
    29041 cgctaccagg cagcgagtgg gaaaaagaag attacctgaa cctcatcgag caactggatg
    29101 aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt gggtctggcg accgggtcgg
    29161 ataacggttg gtacaccctg cgtatcggtg aattaaaagc catgctggcg ctggctggtg
    29221 gcgatctgga acaggctctg gtctggaccg aatggacgat ggagtttaac tcatcagtat
    29281 ttagtccgga acgcgccaac tattatcgct gcctgcaaac gttgttatta ctggcacagg
    29341 aagaagatcg ccagccgctg caatatctga atgcgtttgt tcgcatgtac ggcgcagatg
    29401 ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc gttttacggc ctgcaaccgg
    29461 tagatagcga tctgcacgcg tttgctgcac atcagtcgtt gttgaaggcc tacgaaaagc
    29521 tgcagcgcgc caaagcagca ttctgggcaa aataaaatca aatagcctac gcaatgtagg
    29581 cttaatgatt agtctgagtt atattacggg gcgtttttt
//
LOCUS       DAZFDP010000044        29205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFDP010000044 DAZFDP010000000
VERSION     DAZFDP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            329..1195
                     /gene="folD"
                     /locus_tag="ACHJ1G_002053"
     CDS             329..1195
                     /gene="folD"
                     /locus_tag="ACHJ1G_002053"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4763911.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1197..1409
                     /gene="ybcJ"
                     /locus_tag="ACHJ1G_002054"
     CDS             1197..1409
                     /gene="ybcJ"
                     /locus_tag="ACHJ1G_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4763912.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1517..2038
                     /gene="ybcI"
                     /locus_tag="ACHJ1G_002055"
     CDS             1517..2038
                     /gene="ybcI"
                     /locus_tag="ACHJ1G_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4763913.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2074..3459)
                     /gene="cysS"
                     /locus_tag="ACHJ1G_002056"
     CDS             complement(2074..3459)
                     /gene="cysS"
                     /locus_tag="ACHJ1G_002056"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4763914.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3633..4127
                     /gene="ppiB"
                     /locus_tag="ACHJ1G_002057"
     CDS             3633..4127
                     /gene="ppiB"
                     /locus_tag="ACHJ1G_002057"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4763915.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4130..4852
                     /gene="lpxH"
                     /locus_tag="ACHJ1G_002058"
     CDS             4130..4852
                     /gene="lpxH"
                     /locus_tag="ACHJ1G_002058"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4763916.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4970..5479
                     /gene="purE"
                     /locus_tag="ACHJ1G_002059"
     CDS             4970..5479
                     /gene="purE"
                     /locus_tag="ACHJ1G_002059"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4763917.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5476..6543
                     /gene="purK"
                     /locus_tag="ACHJ1G_002060"
     CDS             5476..6543
                     /gene="purK"
                     /locus_tag="ACHJ1G_002060"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4763918.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6680..7573)
                     /gene="ybcF"
                     /locus_tag="ACHJ1G_002061"
     CDS             complement(6680..7573)
                     /gene="ybcF"
                     /locus_tag="ACHJ1G_002061"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4763919.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALLRIEETLAGEAGTCISL"
     gene            complement(7570..8385)
                     /gene="ylbF"
                     /locus_tag="ACHJ1G_002062"
     CDS             complement(7570..8385)
                     /gene="ylbF"
                     /locus_tag="ACHJ1G_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4763920.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8396..9655)
                     /gene="ylbE"
                     /locus_tag="ACHJ1G_002063"
     CDS             complement(8396..9655)
                     /gene="ylbE"
                     /locus_tag="ACHJ1G_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4763921.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9665..11332)
                     /gene="fdrA"
                     /locus_tag="ACHJ1G_002064"
     CDS             complement(9665..11332)
                     /gene="fdrA"
                     /locus_tag="ACHJ1G_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4763922.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11648..12697
                     /gene="allD"
                     /locus_tag="ACHJ1G_002065"
     CDS             11648..12697
                     /gene="allD"
                     /locus_tag="ACHJ1G_002065"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4763923.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12719..13954
                     /gene="allC"
                     /locus_tag="ACHJ1G_002066"
     CDS             12719..13954
                     /gene="allC"
                     /locus_tag="ACHJ1G_002066"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4763924.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13965..14750
                     /gene="allE"
                     /locus_tag="ACHJ1G_002067"
     CDS             13965..14750
                     /gene="allE"
                     /locus_tag="ACHJ1G_002067"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4763925.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14879..16024)
                     /gene="glxK"
                     /locus_tag="ACHJ1G_002068"
     CDS             complement(14879..16024)
                     /gene="glxK"
                     /locus_tag="ACHJ1G_002068"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4763926.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16046..17346)
                     /gene="ybbY"
                     /locus_tag="ACHJ1G_002069"
                     /pseudo
     CDS             complement(16046..17346)
                     /gene="ybbY"
                     /locus_tag="ACHJ1G_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(17403..18764)
                     /gene="allB"
                     /locus_tag="ACHJ1G_002070"
     CDS             complement(17403..18764)
                     /gene="allB"
                     /locus_tag="ACHJ1G_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO4763927.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18824..20278)
                     /gene="ybbW"
                     /locus_tag="ACHJ1G_002071"
     CDS             complement(18824..20278)
                     /gene="ybbW"
                     /locus_tag="ACHJ1G_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4763928.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20447..21325)
                     /gene="glxR"
                     /locus_tag="ACHJ1G_002072"
     CDS             complement(20447..21325)
                     /gene="glxR"
                     /locus_tag="ACHJ1G_002072"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4763929.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21425..22201)
                     /gene="hyi"
                     /locus_tag="ACHJ1G_002073"
     CDS             complement(21425..22201)
                     /gene="hyi"
                     /locus_tag="ACHJ1G_002073"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4763930.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22214..23995)
                     /gene="gcl"
                     /locus_tag="ACHJ1G_002074"
     CDS             complement(22214..23995)
                     /gene="gcl"
                     /locus_tag="ACHJ1G_002074"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4763931.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24085..24900)
                     /gene="allR"
                     /locus_tag="ACHJ1G_002075"
     CDS             complement(24085..24900)
                     /gene="allR"
                     /locus_tag="ACHJ1G_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4763932.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24978..25460)
                     /gene="allA"
                     /locus_tag="ACHJ1G_002076"
     CDS             complement(24978..25460)
                     /gene="allA"
                     /locus_tag="ACHJ1G_002076"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4763933.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25690..26616
                     /gene="allS"
                     /locus_tag="ACHJ1G_002077"
     CDS             25690..26616
                     /gene="allS"
                     /locus_tag="ACHJ1G_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4763934.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26685..27779
                     /gene="mnmH"
                     /locus_tag="ACHJ1G_002078"
     CDS             26685..27779
                     /gene="mnmH"
                     /locus_tag="ACHJ1G_002078"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4763935.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27895..28260
                     /gene="ylbG"
                     /locus_tag="ACHJ1G_002079"
                     /pseudo
     CDS             27895..28260
                     /gene="ylbG"
                     /locus_tag="ACHJ1G_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29027..29205)
                     /locus_tag="ACHJ1G_002080"
                     /pseudo
     CDS             complement(29027..29205)
                     /locus_tag="ACHJ1G_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7241 a         7870 c         7225 g         6869 t
ORIGIN      
        1 agatgaatac tttcgcgcaa aatatattca acacaaatat agacctgaag cggtaaatta
       61 ccaggctgaa aattcttttt atattgtcag gtatttctta aattatctta atccttaaac
      121 aaggaaataa atcagttcca gatttacaac gccatcatgg acgaaaaatg aagctttcaa
      181 tctcagcgac ctcagcgacg gtgcgcctca ccttcgcaag aggtcgcttc acgcgataaa
      241 tctgaaacga aacctgacag cgcgccccgc ttctgacaaa ataggcgcat ccccttcgac
      301 ctacgtaaca gatggaatcc tctctctgat ggcagcaaag attattgacg gtaaaacgat
      361 tgcgcagcag gtgcgctctg aagttgctca aaaagttcag gcgcgtattg cagccggact
      421 gcgggcacca ggactggccg ttgtgctggt gggtagtaac cctgcatcgc aaatttatgt
      481 cgcaagcaaa cgcaaggctt gtgaagaagt cgggttcgtc tcccgctctt atgacctccc
      541 ggaaaccacc agcgaagcgg agctgttgga gcttatcgat gcgctgaatg ccgacaacac
      601 catcgatggc attctggttc aactgccgtt accggcgggt attgataacg tcaaagtgct
      661 ggaacgtatt catccggaca aagacgtgga cggtttccat ccttataacg tcggtcgtct
      721 gtgccagcgc gcgccgcgtc tgcgtccctg caccccgcgc ggtatcgtca cgctgcttga
      781 gcgttacaac attgatacct tcggcctcaa cgccgtggtg attggcgcat cgaatatcgt
      841 cggtcgtcca atgagcatgg aactgttgct cgcaggttgc accactaccg tgactcaccg
      901 tttcactaaa aatctgcgtc accacgtaga aaatgccgat ctgttgatcg ttgccgtcgg
      961 caagccgggc tttattcctg gtgactggat caaagaaggc gcaattgtga ttgatgtcgg
     1021 catcaaccgt ctggaaaatg gcaaagttgt gggcgacgtc gtgtttgaag acgcggctaa
     1081 acgcgcctca tacattacgc ctgttccggg cggcgttggc ccgatgacgg ttgccacgct
     1141 gattgaaaac acgctacagg cgtgcgttga atatcatgat ccacaggatg agtaacatgg
     1201 cgacattttc tttaggtaaa catccgcacg ttgagctgtg cgacttgctg aaactggaag
     1261 gctggagcga aagcggcgcg caggcgaaaa tcgcgattgc cgaaggccag gtgaaagtcg
     1321 acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc cggtcagaca gtgagttttg
     1381 caggtcacag cgtacaggtt gttgcctgat ccctttcccg gcctgtatca acaggccggt
     1441 tctcttttca gacttttgcc ctctacgccc cgttcatcca tgctgatagc atcgacattt
     1501 tgccgaagcg accatcatgc caaccgttat tacgcacgcc gccgttcccc tatgcattgg
     1561 tttagggttg ggttcgaaag tcatccctcc gcgtctgtta tttgccggaa tcatcctggc
     1621 gatgctgccc gacgccgacg tattgtcgtt taaatttggc gttgcttacg gcaatgtttt
     1681 tggtcatcgc gagtttaccc attcgctggt gtttgcgttt gttgtcccgc tgttatgtgt
     1741 gctgattgga cgccgatggt tcagggctgg gctgattcgc tgctggctgt ttttaaccgt
     1801 ctcgttgcta tcgcacagct tgctggattc ggtaaccact ggcggtaagg gcgttggctg
     1861 gctgtggccg tggtcagatg aacgcttttt cgctccctgg caggtgatta aagtcgcgcc
     1921 gtttgcattg tctcgttaca ccacgccgta cgggcatcag gtgattattt ctgaattgat
     1981 gtgggtgtgg ttgccgggga tggtgcttat gggaatgttg tggtggcgca ggcgataacc
     2041 ggatgcgaaa actcgcatcc ggcaacagcg caattactta cgacgccagg tggtcccttg
     2101 cgggccatct tccagcacga tccccatctc gttaagacgg tcacgcgccg catctgccgc
     2161 cgcccagtct ttcgctttac gggcatccag acgctgttga attaacgctt caatctcagc
     2221 cacttcgctg tcgtctgcct gcgcaccgct ttgcaggaac gcttccggtt cttgctccag
     2281 caggcccaat acagcggaaa gtttacgcag gtgagatgcc attgcattcg ccgctgccat
     2341 atcttctgct ttcagacggt tcacttcacg cgccatatca aacagcacgg aataggcttc
     2401 cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc gcttcaaacg cttcgccacc
     2461 ggcaggcgca acggttttat ctgtgccgcg cagcgcaatg tagaggcgct ccagcgccgc
     2521 acgcgcctgc ttcaggttct cttcgctgta gttcagttgg ctgcgatagt ggcccgacat
     2581 caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc acatcgcgca cggtaaagaa
     2641 gttacccagc gatttggaca tcttctcgcg gtcaaccatc accatccccg agtgcatcca
     2701 gtagttcaca tactggccat catgggcaca ggtggactgc gcgatttcgt tctcgtggtg
     2761 cgggaacatc aggtctgaac cgccgccgtg gatatcaaag tggttaccca gctgcttgca
     2821 gttcattgcc gaacattcaa tgtgccagcc tggacggccc gcgccccacg gagacggcca
     2881 gctcggttcg ccctctttcg acatcttcca cagaacgaag tccatcgggt tgcgtttgtc
     2941 gtcgaccacg tcaacgcgcg cacctgcctg cagctggtcg agatcctgac gcgacagcac
     3001 gccatatgtc ggatcggtcg ggacgtcgaa catcacgtcg ccgttgtccg ccacataagc
     3061 gtgacctttg gcgatcagtt gttcagtgag ttcaataatt tctgcgatat ggtgcgtcgc
     3121 gcgcggctcc atatccgggc gcagaatgtt caaagcatca aaatctttgt gcatttcggc
     3181 gatcatgcga tccaccagcg ccacaaagct ttcgccattt tcattggcgc gtttgatgat
     3241 tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt ttatagccga ggaaacgcag
     3301 atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg tgaccgatat gacagagatc
     3361 gtaaacggtg attccacaca catacatgcc gacttccccg gcgtgaatag gcttaaattc
     3421 ctctttttgg cgtgtcagag tattgaagat ttttagcatc gaagattccg tttagacatg
     3481 tgtgggtaat tgagttgcgt ataataccca tatttcccgc gcgaatcagc atacattgcg
     3541 tgatgatccg atcgtgcggt tatgctataa caccacccta tatatgaccc gaactgggtt
     3601 gaagcaccaa tcaaacggaa caggatgcaa aaatggttac tttccacacc aatcacggcg
     3661 atattgtcat caaaactttt gacgataaag cacctgaaac agttaaaaac ttcctggact
     3721 actgccgcga aggtttttac aacaacacca ttttccaccg tgttatcaac ggctttatga
     3781 ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc caccaaagaa ccgatcaaaa
     3841 acgaagccaa caacggtctg aaaaataccc gtggtacgct ggcaatggcc cgtactcagg
     3901 ctccgcactc tgcaactgca cagttcttca tcaacgtggt tgataacgac ttcctgaact
     3961 tctctggcga aagcctgcaa ggttggggct actgcgtgtt tgctgaagtg gttgaaggca
     4021 tggacgtggt agacaaaatc aaaggtgttg caaccggtcg tagcggtatg caccaggacg
     4081 tgccaaaaga agacgttatc attgaaagcg tgaccgttag cgagtaatcg tggcgacact
     4141 ctttattgca gatcttcatc tctgcgtgga agaaccggcg atcaccgccg gttttctgcg
     4201 ttttttagcg ggggaagccc gcaaggccga cgcgctgtat attcttggcg atctgtttga
     4261 agcatggatt ggcgacgacg atcccaaccc actccatcgc cagatggcgg cagcgatcaa
     4321 agcggtgtcc gattccggcg ttccctgtta tttcattcat ggcaaccgtg attttctgct
     4381 cggcaaacgc tttgcccgtg aaagcggcat gacattattg ccggaagaaa aggtgctcga
     4441 actttatggt cgccgggtgt tgattatgca tggcgacacg ctgtgcaccg atgacgcggg
     4501 ttatcaggct tttcgcgcca aagtccacaa accctggctg caaatgctat tcctcgccct
     4561 gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcga gcgaacagta aagaagccaa
     4621 cagcagtaaa tcgctggcga tcatggacgt taaccaaaac gcggtagtca gtgcgatgga
     4681 aaaacatcag gtgcaatggc tgatccacgg acatacccat cgcccggcgg tacatgaact
     4741 tatcgccaac cagcaacctg cttttcgcgt ggtactcggt gcctggcata cggaaggttc
     4801 gatggtgaaa gtcacggcgg atgacgttga gctgattcat tttccgtttt aaaaaacccg
     4861 caactttgct gatttcacag ccacgcaacc gttttccttg ctctctttcc gtgctattct
     4921 ctgtgccctc taaagccgag agttgtgcac cacaggagtt ttaagacgca tgtcttcccg
     4981 caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa agcgactggg ctaccatgca
     5041 gttcgccgcc gaaatcttcg aaatcctgaa tgtcccgcac cacgttgaag tggtttctgc
     5101 tcaccgcacc cccgataagc tgttcagctt cgccgaaagc gccgaagaga acggttatca
     5161 ggtgattatt gcgggcgcag gcggcgcagc gcacctgcct ggtatgattg ccgccaaaac
     5221 gctggtgcca gtgctgggcg tgccagtaca gagcgccgca ctgagcggtg tcgatagcct
     5281 ctactccatc gtacaaatgc cgcgcggcat tccggtgggt acgctggcga ttggtaaagc
     5341 tggcgcggca aacgcggcgt tactggcagc acaaattctt gcgactcatg ataaagaact
     5401 gcaccagcgt ctgaatgact ggcgcaaagc ccagaccgac gaagtgctgg aaaacccgga
     5461 cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta acgggcagtt aggccgtatg
     5521 ctgcgtcagg caggtgaacc gttaggcatt gctgtctggc cggtcgggct ggacgctgaa
     5581 ccggcggcgg tgccttttca acaaagcgtg attaccgctg agatcgaacg ctggccggaa
     5641 accgcattaa cccgcgagct ggcgcgtcat ccggcctttg tgaaccgcga tgtgttcccg
     5701 attattgccg accgtctgac tcagaagcag cttttcgata agctccacct gccgaccgca
     5761 ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg tgtttgagcg tttaggtgaa
     5821 ctggcgattg ttaagcgtcg cactggtggc tatgacggtc gcggtcaatg gcgtttacgt
     5881 gccaatgaaa ccgaacagtt accggcagag tgttacggcg aatgtattgt cgagcagggc
     5941 attaacttct ctggtgaagt gtcgctggtt ggcgcacgcg gatttgatgg cagcaccgtg
     6001 ttttatccgc tgacgcataa cctgcatcag gacggtattt tgcgcaccag cgtcgctttt
     6061 ccgcaggcca atgcgcagca gcaagcgcaa gccgaagaga tgctgtcggc gattatgcag
     6121 gagctgggct atgtgggcgt gatggcgatg gagtgttttg tcaccccgca aggtctgctg
     6181 atcaacgaac tggcaccgcg tgtgcataac agcggtcact ggacacaaaa cggtgccagc
     6241 atcagccagt ttgagctgca tctgcgggcg attaccgatc tgccgttacc gcaaccggta
     6301 gtgaatagtc cgtcggtgat gatcaacctg attggtagcg atgtgaatta tgactggctg
     6361 aaactgccgc tggtgcatct gcactggtac gacaaagaag tccgtccggg gcgtaaagtg
     6421 gggcatctga atttgaccga cagcgacaca tcgcgtctga ccgcgacgct ggaagccttg
     6481 atcccgctgc tgccgccgga gtatgccagc ggcgtgatgt gggcgcagag taagttcagt
     6541 taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg ccttatccgg cttaccgctc
     6601 cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga
     6661 ttgccggatg ctctcatcac tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct
     6721 cttcaattcg caataacgcc ccaatccacg cgggtttacc acggcttctg acataaccac
     6781 ttaccgccgt taccttcggc cccatcgaac catcggcttt ggtaaatggc gctaactcat
     6841 ccggtgtggc atggcgaatg gcgcgttgct gcggcgttcc ccagttttca taaaccgcgt
     6901 cagcatcggt gaggatcacc agtccatcgg cattaatctg ctcggcgagc aacgcagcgg
     6961 cgagatcttt atcaatcacc gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc
     7021 cgccgccact gcaaatcacc acatgcccct ctttgagcaa caactcgatg gcttcgctgt
     7081 cgagaatttt acgcggttgc ggagacgcca ccacccggcg caaatattta ccatcacgtt
     7141 tcatctgcca gccgtaagcc gcttccagtg ccttttgttc ttctggctga taaaccggac
     7201 caataaattt ctctggctgc aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca
     7261 gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa actctgcgcc agcatatagc
     7321 caatcatccc ctggctttcc gccaccagca catccagcgg atacggatct acctctttcc
     7381 acgccagatt ctgcaatgcc agcagcccca cctgcggccc attgccgtga acaatcgcca
     7441 gccgataaga acgggccagg cgcgccagcg cgggtacagc actggcgata ttgcgatatt
     7501 gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca agagctacaa
     7561 ccagtgtttt catggttttc cttgtaataa ttgttggcca agccagaacc ccagcagcgt
     7621 atctgcccct gacgtatgcc ccagcgccag cagcgaatca atcgcaacag cggtacgctt
     7681 cgggcaactc agagcatgga caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc
     7741 ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt tgaagattgt cggaacaggc
     7801 aaaaaacggg cgaccggcgc gcgcatctaa agcgccataa taccaggccg ccagcagcat
     7861 tccgctcaac gtgtcatcat gacttggcgt taatccgggg cctttaccca gccagtgccg
     7921 ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc
     7981 agcgggcagc ggatcgcttg ccgccatcgc cagtggtccg aaaagtccgg tttcctccgc
     8041 gcgttgcatc catgcagctg caagcggttg aggatgcgaa ggcggcgtaa tacgcagcaa
     8101 acaataacgc tgtggctgtt taaccgtgaa acgcccgagg cgaatccctt gagccacaac
     8161 ctgtggtcgt tcattgccgc ataatccacc gcataacgca tcgaattgcg cgcggcgaag
     8221 cacccatcct ccggggccga aaccactacc ctgacgatgc agcgttaaca gttcgccgct
     8281 ttccgtcatc aggttaatcg cccgtcgcca cactcccgct aaccgccagg actggcgata
     8341 attcggtgca ctacggctgg caagcagagg atggatgatc gtcattgcgc gttcctcaac
     8401 caatccccat gctttcagcc agtgcttcca gcgcctgttc aaaacacgcc agcggtgccc
     8461 gcacagtgcc tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata ccggtattga
     8521 tgagtggcgt aatgccggtt tctaccacgc gacgaatatc cagccccagg cacgcgccct
     8581 ggaaatccca gccggggatc tgcaactgca tattgcgctc aaggtaaatt tccgccatct
     8641 cttcagatac cgctctggcc gcttccatgc cacccgcacc gacaaagcgc gttacgccag
     8701 gcgctgcgat cattgccgca cctccgatac caaaggtttc ggtaatcgcg ctatcgccca
     8761 tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta
     8821 caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg aatcccgaac atattgccgt
     8881 tgcgggtcat tgccgtgacg atgctgcctg cgcggatcat cgcgccagca tccatcgccg
     8941 ccttgcagta agccatcgcg aggttgagga agaactgatc ggtcacgctg aggaaatcca
     9001 tcacttcggc gatgtgctgt ttatcatgat cgaggcgagc aatttgtggg gccagcgcac
     9061 gcattaacag tgcagaggaa gcaatattgc gttgatggaa ctcatcgccc atcgtaatgc
     9121 cctgcgccat catcgccgtg agatcgatac cgcgctccat gcgccccagc gccgcgctta
     9181 atactggcat taacacatcg cgcatccagc gatggcgagt caggacatct tcgccgtaag
     9241 cgccaaaacg catcactttg ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt
     9301 cggtcacgtt ctcgaccacc agcatcggca tactggcaga agtaataccg cccattggac
     9361 cgacggcatt cacatggtga caaggaatga agttcacttc cccctgctcc agtattgcca
     9421 gcgcctgcgc ttcatctttc gcccagcctt cgaacagaca tgcgcccacg cacgcccctt
     9481 tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc
     9541 cctcgtttag ttcgctgata agtgaagaag ccggttgcac atccagccag tgtggacgag
     9601 cacgacgaat ttgttcgata accgcagcat tggcttgcgc cactgatgta aacatgggaa
     9661 ccccttattg caaacgttct aataaacgag ccagtttttt attgccgccg gcgactggcg
     9721 accattggta atgcacaacc ggtttgctgg cgctttgtag ctccagcgca aagctgcgta
     9781 atccgatgtt aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc tgtgtggcag
     9841 acgagagcgg atgaattaac gctgccgcca gcaaggtggc ttccggtagc gaactcacta
     9901 ccgcgatccc cgcatcttcc agcgtggcga tttgctgcga gcggcattgc gggtcacgtt
     9961 cggtacctgt caccgtggca atggcgtaca gcggttggct gtctgaacgc gcggcacagg
    10021 ctttttgcca ggcgctcacc agcgaggcag caggatcggc ggtcgcaccg aagccaatca
    10081 cgacatcaag cagcaacacg cgcacttgcg gtttagcgcc gagatcggca attaactggt
    10141 tgcgtaaggc tgggtcgatc atcggatggg gacgcccgac ggtgtagaaa tcatcgccca
    10201 gatcaatgat ctggtgacca tcggcgtcca gcatcatgcc gtgatgatgg gtatcgtcgg
    10261 cttccacgcc aaggtgcccg gcaagtaatc ccgccgcttc ggcagcaagc gtaccgccgg
    10321 tatacaaacc gcaaataaat ccgctgctga caggcgctat tgcgttacgt cgcgccgtga
    10381 cgcgtgaaag caggcaagcc aggcgtgcgg cctcatccag cgaggaggca aaccagacat
    10441 tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg
    10501 ttgctttcat ggcattaaca attttcagac gcacagcttc ggcaggtggt tttgaaacaa
    10561 atgccagcac ttcgcttttc tcgtctgcac tgagcatttc cagcgctgtt agcgcactga
    10621 tgccgcccac ttcacggctg aggtcgcgcc cgccaaggcc aatcgcgtga gtaattccct
    10681 cccctgccag cgcaatctgc gaacacagct cctgaatccc ggtaccggaa gcgccaatga
    10741 cgccaatatt gccttctggc atcacgttag caaaagccag cggtgtgccg gcaatcatcg
    10801 acgtaccgca gtccggcccc atcaccagca agcctttttc ccgcgcgcgg gttttaagtt
    10861 ggatttcatc ttccagcgtg acgttatcgg agaacatcat cacgttgagg ttgcgatcca
    10921 gcgcctggtt tgtcagctcc gccgcatact cgccagccac tgaaatcagc gccagattgg
    10981 catcgggtaa tttctgacag gcgctgtccc agcgacgcac ctgcgtcaac gcctggctgc
    11041 tgcctgaccc ctgcgccagt tgttttagcg cctcttcaag ctgctgcata atcgcctgcg
    11101 cgatccccgc atccgccgct tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt
    11161 tattaaaatc gtcatgccag aaacctgtgg tatctaataa cgctttattg gcgggcgtac
    11221 ccatcattac ggaaacatca tcaacatttt ctgattcgct gagttttcgt gaaataatca
    11281 ttaaactgac cgaatcctga aaacaccctt ttttaataaa ggcgtggatc ataacaactc
    11341 cttaaaaatt gcacccatgg caattgcacg agcagtgtta atgtggcgat tacggtatcg
    11401 atgacaacga gtaaataagg tgatgaatat cacacaggga ttattaacaa aagaattcaa
    11461 ataaccgcca ggctattacg cattattttt taataagcgt ggttgtttta aaaatcaggt
    11521 taaaagcgcg aagattatca aataaattct aactatattt tttgcctgtc tggatcacat
    11581 aatccagata ttttccctgt atgttaattg cagtcatgct tcacaccgtc gttaaaaagg
    11641 aagacagatg aaaatcagtc gggaaacact ccaccagcta attgagaata aactctgcca
    11701 ggctgggtta aaacgtgagc acgctgcaac cgtggctgaa gtattggttt acgccgatgc
    11761 cagagggatc cactctcatg gcgcggtgcg cgtggaatac tacgcggaac gcatttcaaa
    11821 aggcggcacc aaccgcgaac cggagtttcg tcttgaggaa accgggccgt gctcggcaat
    11881 tttacatgcc gacaatgccg ccggacaggt cgcggcgaaa atgggtatgg aacatgccat
    11941 caaaaccgcc cagcaaaatg gcgttgcggt ggtcggtatc agccggatgg gtcacagcgg
    12001 cgcaatctct tattttgtgc agcaggcagc ccgcgccgga ttaattggta tttcgatgtg
    12061 ccagtccgat ccaatggtgg tgccgtttgg cggcgcggaa atttactacg gtactaaccc
    12121 actggccttt gccgcgccgg gagaaggcga cgagatcctt acctttgata tggcgactac
    12181 cgtacaggca tggggaaaag tgctcgacgc ccgctcgcgt aatatgtcta tcccggatac
    12241 ctgggcggtc gataaaaacg gtgcaccaac aaccgatccg ttcgcggtac atgctctgct
    12301 ccccgccgcc gggccaaaag ggtatggcct gatgatgatg attgacgtcc tctcaggcgt
    12361 cttactcggc ttaccgttcg ggcgacaggt tagttcgatg tatgacgatt tacacgccgg
    12421 gcgtaatttg gggcaattac atatcgttat taacccgaac tttttcttct ccagcaaatt
    12481 atttcgtcaa catcttagcc agaccatgcg cgaattaaat gccattaccc ccgcgcccgg
    12541 ttttaatcag gtttattatc ccggacagga tcaggatatt aaacaacgca aagccgccgt
    12601 cgaaggcatc gaaattgttg atgatattta ccagtattta atttccgacg cgctttataa
    12661 cacgtcatac gaaacgaaaa atccctttgc gcaataatta ttgagacagg actttcttat
    12721 gattacacat tttcgtcaag ccatagaaga aacgctgccc tggctttcct cttttggcgc
    12781 tgacccaacg ggtggtatga cccgtttact ttattcgccg gaatggctgg aaactcagca
    12841 gcaatttaaa aaaagaatgg cagcaagcgg gctggaaaca cgtttcgatg aagtggggaa
    12901 tttatatggt cgcctgagtg gcaccgaata tccacaagaa gtggttctga gcggttcgca
    12961 tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc tggcggcgtg
    13021 gctggcaatt gactggctga aaacgcaata cggcgcgccg ctacgtacgg tcgaagtggt
    13081 ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca gtaaaaatat
    13141 ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag gaaatagttt
    13201 tgtcgatgcg atgaaggctt gcggatttac tcttccgaac gccccactaa ctccgcgtca
    13261 ggatattaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg aaagtaatgg
    13321 gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgttatacgg taacgctgaa
    13381 cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata cagtttacgc
    13441 tttcagtcgc atttgccatc agtcggtcga aaaagcgaaa aggatgggcg atccgctggt
    13501 tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc cgggtaaaac
    13561 cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt tcacccaaca
    13621 gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg atatcgattt
    13681 atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc tgacagaatt
    13741 gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc acgacgcgca
    13801 aattttcgcg cctcgcgtac caacctgcat gattttcatt cccagcatca acgggatcag
    13861 ccataacccg gcggaacgca ccaatattac cgaccttgcc gaaggggtca aaacgttggc
    13921 actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga tatttaaata
    13981 acgtcaccgg ttaccgcgat gatttactgg ctaaccgtgc gattgttaaa cacggtaatt
    14041 tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt gaaaattgtg
    14101 acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat ctggtcacac
    14161 tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa acgttcctgt
    14221 atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc ttaagcgaag
    14281 gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc caggccgaag
    14341 acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat gcaccgtggc
    14401 tggtttctgg taatgccagc gaactggaac gcattcacta tgaaggtatg gatgatgtga
    14461 tcctgctgga tttcctgccc aaagagttag gttttgatat gaacatgcat atcctctctt
    14521 ttgcgccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat ggtgcctata
    14581 ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg aaaaaaggcg
    14641 attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg cgtggtgaag
    14701 cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa gtcataccac
    14761 tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg gctttttttg
    14821 tagtaaattc aggtaaaaaa agatgcgaaa taaatcgcat ccgctttaaa ggtaagggtc
    14881 agtttttaat tccctgacct attttaatgg cgcaggcaat atttcgcgcg ctgttgaaga
    14941 gattggtttc accgctggcg agcacttcgg ctaaaggtgc cagacgaggc aaaatgctga
    15001 ataccgcgtc aatgccgtac tggtgcacca cttccacgcc atcacccaat acgccagcaa
    15061 tcccaatcac cgggacatta aactgcttcg ccaccgacgc aacgcccagc ggcgctttac
    15121 cgcctgccgt ttgcgagtca atgcgccctt ccccggtgat caccagtgct gcgccctgca
    15181 ccgcctgctc caggttgacc gcccgcagta caatctcaat acccggttta atatccgcat
    15241 tgaggaatac cgccgccgca atacccatac cgcccgcagc gccgccgccc gccatctggc
    15301 agacattaat ttcagtttgc tgttgtaaaa cacgggcgta attttgcagc ccctgttcaa
    15361 gttcttcgac catctccggc gttgcccctt tttgcgggcc aaacaccgcc gccgcgccgc
    15421 gtgcccctac cagcgggtta tcaacgtcac atgctacttc aatatggcaa ttcgccagac
    15481 gcggatcgca ttcatccatc tcaatgcttg ccacgcgcgc taaattaccg ccgttagccg
    15541 ccagcacctg gccgtcggca tcaaggaaac gcacaccaag cgcctgagcc atgcccatac
    15601 cgccgtcgac cgtcgcactg ccgccaatgc cgagaataat atggcgaatg ccgttatcca
    15661 gcgcatgacg aattaactcc cccgtaccaa aactggaggc cagcaacgga ttacgctttt
    15721 caggcgcgac cagcatcagg ccacttgctg ccgccatctc aatcaccgcc gttttcccgt
    15781 cgccggtaag gccataaaaa gcattcactg tttcgcccat cggcccgcag acttcaagcg
    15841 tcacgatgtt gccgcccgtc gcggcgacca tcgcatccac cgtcccttcg ccgccatccg
    15901 ctatcggcaa acagatatag ttcgcatcgg gaaagagggt cgaaaacccg gctttaattg
    15961 cctgacaaca tttttctgca cttaagctct ctttaaaaga gtccggcgca atgacaatct
    16021 tcattatcaa tcctttttca acaaattatt cgatgcgttc ccacggaata agattatcca
    16081 taagaacagc cagtaaaatt ccgaccagta agccgttact gagcagagga cgcagcgtta
    16141 atggaaggtc ttgcagatac acaggcggta atgccattaa aaatatgccg acaaataacg
    16201 gcaatgcgag tcgataaata ttgcgagcgg taaaggttat ttgctggcta aacaccagcg
    16261 cggaaaagag taaaggcaga taagaaacca gcatgaccgc gctactcacg ggtaaaggga
    16321 tactgcaaaa cagtcgtgtg agtgccggaa ccagcgccac cagcaggcaa ataacgctgc
    16381 cataaataaa cgaacgccgc gtgtaatcgc ctgtctgggt taataaacca atggatgaaa
    16441 caaacggtga aaatggaatt accgccagcg gtacggttat cagcgtcata aatccggtcg
    16501 ccacaaagct gcgacgataa cgcgtattcc cgcgccctgc tgcggataaa aaacatccgt
    16561 gccccgaatc gcaccgtagg tattgctgat attcaccaga cctgtaatca ccgccgtcag
    16621 aataattccc ggagacaaag cacctccact gccgagcggg aaccactgcc agtgcaactc
    16681 accggagagc gagtgcgaag aaggaaagca aaagtaccac aacaaccagc cggttatggt
    16741 gccgaccagc aggccataac gggcaaaacg ttgcggcagg aagataatca tccccagtac
    16801 caggcacatc accgccaccg agagcgcgaa cggcggtaac tgaattttaa aatttgggtc
    16861 ggctatgcca aacggcagcc cgagcatacc tttgaaaaag atagtggtca gctgcgcgcc
    16921 cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca ggcgatggcc
    16981 taatccgcta aaaccaatca acatcgtcag cacgccggag agcgcaatcc ccaccgccag
    17041 gctggtagcg atatcgttga tcggtgtccc gcgcgatgct tcgccaaggg tgatagtaag
    17101 gatggttccc caccacaggc cacccggctc ttccataata gcgcgacgat gaccgcagaa
    17161 cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga gcgtaagcag
    17221 gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa ccgtgttgca
    17281 aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctca ctgcaaaatt
    17341 gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt gcaggggcca
    17401 gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa gccctgttca
    17461 atgtcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc aatggtacgg
    17521 ccaacatacg ggctgacttt gtggcgatat tccagatcgt cattggtaag aacatagctg
    17581 ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg gcctttttgc
    17641 tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat tggcagagac
    17701 attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg cagaccggca
    17761 ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca tggtgagtgg
    17821 tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc tttctggttt
    17881 tccagatcgc ggatcggcgg tgaacacttc gccagagtgc cgatttcttc gaactgatcg
    17941 gtatccagta caaagtaatg cgggcaggat tcacaagtaa cgtcctgacc ttcctgacgt
    18001 gcacgagtca cttcttcaac gccttccggg ctgctgatat ggcaaacgtg cagacggcaa
    18061 ccggcaactt tcgccagata cagcacgcgg cgaatcgctt ccacttcggt aaataccgga
    18121 cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc ttcgcccagt
    18181 gcgtcacaga tcagcgcgtt ttcgcagtgc accagcaccg gctgccccag ttcgcccagc
    18241 ttctgcgcac ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt gtcgataccg
    18301 cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc atccagctca
    18361 tgcagacggt cgatgttgta agacaccagg ccaccgagtt gtgccgcatc gatagtcagc
    18421 ttgcctttag cggcatcgaa cttcagttca attgaagcgc ggtcaaccgt tgcaggcagc
    18481 tggttgagcg gcatttcgat catggtggtg ataccacctt ttgccgctgc gcgagtaccg
    18541 gtttcataac cttcccagtg gctacgaccc ggttcagaaa tatgggtgtg cgcatcaacc
    18601 atgcccggcg aaaccaccag accagacgca tccataactt cttttgcatc gcccagatcc
    18661 tgaccgatag cagcaatttt cccgccttta acggcgatat ctacaacgcg agcttcgttt
    18721 tctaaaataa cggtgccgtt tttaatgatt aaatcaaaag acataacaaa ctccttgtaa
    18781 aatacagatt ggtttttaac tcaatgttca atatcgggat taattaacct atggtttttt
    18841 gctctcctgt tttttctgct gttgtacgtt tctttaataa ggcgtaggcc gcaaaggcga
    18901 cgatgacgcc gacaaaccat gaaacacgcg ataacggttc cataaagtga ataaacttac
    18961 cgccaagaga taaaataacg gccaccagag ttactgaaaa cgcagtgagg ttaaaaccgt
    19021 tatcgtaata tttataatcg ccaggtgcgg tatacagttc atcaagatta atttgtccgc
    19081 gcatcaccac aaaataatgc gccatcatga caccaattac cggaccaagc attccgccga
    19141 taatatcgag gaaaagataa atgctgtcct gattttccat taatttccac gggcagatca
    19201 gcaagctgat aatactggca atcagtacgc cgtttttata ggtcagtttt gtcggtgcaa
    19261 tggcggcaat ctgataaccg gctggaataa tattaccggt cgcgttagtg gagatagttg
    19321 tcatcagaat aaccagtacc gcaaagaacg aggcgaacag gctgtcccaa cgctgaacaa
    19381 tatccagcac gttccaggta tcagcgccgt agtgaatact ggctccggca ataatacata
    19441 ccccggcgac cgcaaacaga atataggcca caactaaacc cagcgtttgc cccagcgcct
    19501 gctcacgaaa cgagtgggcg ttttgcgtaa agtcggatgc gctcaccgcc ggtgccgccc
    19561 agaccgcaac taccgcgtta atcaccacca ggaacaggaa gccaccgttt tctgctttct
    19621 gaataccgct cggaatgtag tcaaagattg gaccgatccc gaccagtgaa atcgcccaaa
    19681 tcgccatacc gccgaaaacg atatagatgc acgggttaag aatggcagtg aatttattta
    19741 aaactttgcc accgccaaaa cctataccaa cgttgaccag ccagaagatt aagaaagtaa
    19801 ttaagcccgg tagagaaagg cctaacagag tgaaatcacc accgagagtt aaaaatcccg
    19861 gccagatttt gccaatcaga atcaagcagg ccagtgaccc cgcgtaacat tgcaaaccaa
    19921 accacatgat ggcggcaatt ccgcccctta ataatccggg aaacagtgca ccacgtacac
    19981 cgtaagaagc acgcaggatc atggcaaaag gcacaccgta tttactgccc gcagcaccgt
    20041 ttaataccat taccgcggca atgaaaaagg cgctgaggat aattgccagc ataatactaa
    20101 aggtagacaa gccgagaata aaaaagccgc cgaccatcac ataattggga acgttatgaa
    20161 ccgaacccat ccataaggta aaatagttaa atgttttcca ggtccgctgg ctttgcgttt
    20221 tcggcaatag atcttcgcta tagccgcgtt gctggaatag ttttctctga tgttccataa
    20281 atccctctta atttcacgta tcgaataacg ttgttttaat ctagctatga ggaacaaata
    20341 aaataaaaat attattttta atactgaagc aacatctttg gctgctttct tttttaatat
    20401 tgctggccga ttcattttct gatatcggcc atttttattg cgggtatcag gccagtttat
    20461 ggttagccat taattccagc gcctgcacta acgcagagtg atccaactgg ctgccaccgt
    20521 ttgccgcgca ggtattaaat aactcctggc aggtcgcagt gtttggcagg ttcagcgcta
    20581 gcgctttcgc actttgcagt gccaggttga gatctttctg gtgcagcgca attttgaaac
    20641 ctggattaaa agtgcgttta atcatgcgct caccgtgaac ttccagaata cgtgaggaag
    20701 caaagccgcc catcagcgcc tggcgcacac gtaccgggtc cgcaccggct tttgaagcaa
    20761 atagcagggc ttcagaaacc gcttcaatat tgagcgccac aataatctga tttgccactt
    20821 tgcaggtttg accatcgccg ttaccgccca cgagggtgat atttttaccg agcagttcaa
    20881 acagcggttt aacacgttca aataccgctt catcaccgcc aaccataatc gacaacgtcc
    20941 cttcacgcgc accgatttca ccgccggaga ctggcgcatc gagataatcg ccgcccagtt
    21001 cattcacctg acgagcgaaa cgcttagttt caatcgggga aatggagctc atatcaacaa
    21061 tggttttgcc cttcagcgag gctttggtac aaccattttc accgaacaga acttcttcaa
    21121 cctgtggtgt atctggcacc ataataaaaa tgatgtccga tgcttccgtt acctggcgcg
    21181 cagtttcaac actgacggca cccagtgaca gtaattcatc agcaaccgga ccaatggttg
    21241 tgacatgcag ttgatgaccg gcacgcgcca gattaatggc catcggtgta cccataatgc
    21301 ctaagccaat aaatcccagt ttcataatta acctctttta aatttcgctt ttcctgaatt
    21361 cagacaacac gatcccctgc caacttagcg gcctaaaaaa gcattgcctg aacagcgtta
    21421 cgttttaacg gtacggatcc atccagcgta aaccggcttc cgtggtggtt tggggtgtat
    21481 attcacaccc aacccaaccg ttgtagtcag aattttcgat taccttaaag agataatcat
    21541 aattaatttc tccggtcccc ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac
    21601 caattttatc ggcccactga gtcatggtgt tggttaattc accttccatc cgctgcatgt
    21661 gataaatgtc atactgaatt ttcaaattgc agcaaccaac atcatcaatc aatttcagcg
    21721 cctgccgagt tccggtgaga tggaaaccag gaatatcaaa atggttaata ggttcaatca
    21781 gtaataaaat atcttctttc atcagcatat tcgcggcata acgcaggttt tctacaagcg
    21841 ttgcgtgaat ctgttcactg ctgaaaccag ccggcgtttt accgaccaga cagttaattt
    21901 ttttattacc cagcgcacgg gcataacgaa tcgctgctgc tacgccatcc cgaaactctt
    21961 cttcacggcc aggaatacag gcaataccgc gctcccccgc cgcccagtca cctgccggta
    22021 aattgtgcag cgtatgttcg agtttattac tcgccagcac ctgttttaat tcttcaatgt
    22081 cgtagtcata aggaaacata aattcaacgc cgcgaaaacc acactgcgca gctttctcaa
    22141 aacgggcgag aaaatcatat tcaccaaata acatcgataa attagcagag aaacgtaaca
    22201 ttatttatct cccttattca tagtgcatga agcaggtttc agtcggtgcg tccgctgcgt
    22261 tatcggcgat atcttcaaat tccatgacgt tatccagttc gctgcccatc gaaatattgg
    22321 tcacacgctc aagaataact tccacgacta ccggtacccg atattgcgcc attaaggctt
    22381 tcgcctgttc aaaggctggc gcaatatctt ccggtttgaa gacccgaata gctttacaac
    22441 ctaaaccttc cgctactttt acgtggtcga cgccgtaacc gttcacttca ctggagttaa
    22501 tattttcgaa cgcgagctgt acgcagtaat ccatgtcaaa ggcgcgttgt gactgacgaa
    22561 tcagccccag ataagcgtta ttgaccagta catggatgta cggaatgttg aactgtgcgc
    22621 caacggctaa ttcttcaatc aggaactgga agtcgaagtc accggaaatc gccaccacat
    22681 tccgttctgg atcagcagca cacacgccca gcgccgccgg aatggtccag cccagcgggc
    22741 ctgcctgacc acagttgatc cagtgacggt ctttaaagac atgcagcatt tgtgccgcag
    22801 cgatttgtga caggccaatg gtggtgacat aacaaacatc gcgaccaaag gctttgttca
    22861 tctcttcata cacgcgctgc ggtttcaccg gcacgttgtc gaagtgggtt ttgcgcagca
    22921 aagtgcgttt gcgctgctgg cagtcggcga cccattcttt acgacacggc agacgacccg
    22981 ctttttgcat ctcctgcgcc acttcaacca gcagtgtcag cgccgcttta gcatcagaga
    23041 caatgccgag atccggacac agcacgcgac caatttgcgt cggctcaata tcgatatgaa
    23101 cgattttgcg cccttcggtg tatttctcta ccgaaccggt atgacggtta gcaaaacggt
    23161 taccgatacc aaacaccatg tcggacgcca gcagcgttgc gttaccgtaa cgatgcgcgg
    23221 tttgcagacc caccatcccg gccatcagtt catgatcgtc cgggatacag ccccagccca
    23281 tcagcgttgg gatcaccgga acgctggtca gttcagcaaa ctgttgtaac agtgcagctg
    23341 cgtcagcatt aattaccccg cccccggcaa caatcaccgg acgttcggcc tggattaaca
    23401 tttctacagc tttttcgatc tgcatacggc tggcagcagg tttgtagacc ggcagcggtt
    23461 cgtacatatc aggatcaaac tcgatttccg caacctgaac gtcgaacggt aaatccacca
    23521 gtaccggacc cggacgacca gaacgcatca ggtgaaatgc ctgttgcaac acacgaggca
    23581 ccagcgccgc ttcacgtact gtaaccgcca ttttacttac cggcttggcg atagcttcga
    23641 tatcaacagc ctggaaatct tctttatgca gacgggcgcg cggagcctgg ccagtaatac
    23701 acagaatagg aatggaatca gcagaagcag aatagagcgc ggtgatcatg tccgtgcccg
    23761 caggaccgga agtccccaga catacgccga tattccctgc cgttgcgcgg gtataacctt
    23821 ccgccatgtg cgaagcacct tccacatgac gcgccagaat gtgacgaata ccgccgtgct
    23881 tacgcatcgc tgagtagaac ggattgattg cagctcccgg aacgccgaag gcggtggtga
    23941 taccttcttt ctccagcaca tacattgccg cgtcaacggc tctcattttt gccattttat
    24001 tccaacctct attaattgga aaaattttcc aactttcaaa ttttgtcaaa aaaacccgct
    24061 ccgcagagcg ggatgcgaca gacactatgg atgtgctttc agtcccaacg ccgtgctgat
    24121 atcgcgggcg gtgtctctga ccagctcacc ctgactgaca aaacgatctt ctgtcagtct
    24181 tgatgaaggc ccggagatgg agatagcggc aacaacgcta ccgacatcat cgtaaattgc
    24241 tgaagctatg caattcagac ctacaacatg ctcttcttta tctacggtat agcccagttc
    24301 acgcgcttgt tccaggtcct tcagcaaggt gggcatatcc acaagcgtag ttggcgtaaa
    24361 ctgctgcaaa ccggtttgca gaatgatgct catcaactcc tcttccgcca gcggataaag
    24421 caacgccttg cccgcaccgg aagcatgcag cggcagacga ctgcccagtg gcgcacacat
    24481 cctgaccatc gatttacact ctaactgacc aattaatacc gcttcattgc cgttacggat
    24541 cgcgacattg accgtttcgc cggaaagtaa catcaggcgg cgcataaacg gcccggcgac
    24601 ggagaggaca tcgcggttat ggatgtacgc cgcaccgacg ttaaagacac ctaatcctat
    24661 atgccaccag cctaactgac tgtcctgata gacaaaatcc gctgcctgta aaaccttcag
    24721 caagcgaaag gtcgtagaga gcggcaaatc cagattgaga gaaatatcgc taaccgacga
    24781 acttccccca cttttttcca gatattgcag aatcgcaatt ccccgctcta acgcctgtgc
    24841 gcccttctgt gccacaggct ccgcctgtcc tggcctgccg cgccgtctaa cttccgtcat
    24901 acctacgtta actccgacct acaagtgagc ataaacaatg aacaattagt ttatcttatg
    24961 caagtcggtt gcaggcgtca cgcaaaacag agttcctgtt caggaatact ttcaacatca
    25021 cagttgtcac tgccgccgcg atcgatggtc agaaaatcgg tgacgcgctg ccaggcgaaa
    25081 agtgggtgat gccagacgtt acggtggtaa ttcactccct gttcgccgtt ggtaataaac
    25141 gcccgcaggg ttgacaggtc tggtttgtcg tcacctaacg ccacgaccac cacaaacacc
    25201 tcacctttca tcgggataaa ggcctgagta cccagcggat gacgttcgag ttcgtgaatg
    25261 gtcagcggca gattcgccgg ttgcgcgcgg ttaatgctga taagcgtacg gtcttgctca
    25321 agaatctcaa ccagcgccaa atcgtggtaa cgctccacca ggccattgtt aatatggaaa
    25381 aaatcccgtt gctgcgtttc gattacgtcg ccataagcac taaaggcttc ctgacttaac
    25441 ggtaatacct gaagtttcat ctttttgtcc ttgtaacata attccgttca accaccgccg
    25501 accggtcagc ttggtttcaa taacaggcaa tctaaaacct taagagaaca acatcaatac
    25561 aaaagttgga atatttttcc aaaactagat ttagtccaca aattgaacag ggttatcatg
    25621 taaaaatact gataaatcag aggataagaa aatcacgcaa cttgcggatt taaaagggga
    25681 tacctgaaga tgttcgatcc agaaaccttg cggactttca ttgcggttgc tgaaacagga
    25741 agtttttcaa aagcggcaga acgattatgt aaaaccacgg cgacgatcag ttatcgcatt
    25801 aaacttctgg aagagaatac cggagtagcg ctgtttttcc gcacgactcg cagcgtgacg
    25861 ttgacagcgg ctggcgagca tctactttcc caggccagag actggctgag ctggctggaa
    25921 agtatgccaa gcgagctgca acaggtgaat gatggcgtgg aacgccaggt gaatattgtc
    25981 atcaacaacc tgctctacaa cccccaggcc gtcgcccagt tgctggcgtg gctgaatgaa
    26041 cgttacccct ttacccagtt tcacatctcc cgacaaatct atatgggcgt ctgggattcg
    26101 ctattatacg aaggtttttc actggctatc ggcgtcacgg gaaccgaggc gctggcaaat
    26161 acctttagtc ttgatccctt aggatcggtg caatggcgct ttgtcatggc ggcggatcat
    26221 ccgctggcga acgttgaaga gccgctaaca gaagcgcagt tgcggcgctt tccggcggtc
    26281 aatattgaag acagcgcccg caccttaacc aaacgcgtcg cctggcgatt gcctgggcaa
    26341 aaagagatta ttgttcctga tatggaaacg aaaatcgccg cccatcttgc gggcgttggc
    26401 attggttttt tgccaaaatc gctttgccag tcaatgatcg ataatcaaca actagttagc
    26461 cgggtaatcc caacgatgcg ccctccttcg ccattgagtc tggcatggcg caaatttggc
    26521 agcggcaaag cggtagaaga tattgtgacc ttgtttaccc agcgcaggcc ggaaatcagc
    26581 ggatttttag aaattttcgg caacccacgc agttaaaaat caagtttcct tgcgcgttgg
    26641 cgcgtaaaat agcgcgcaag ctgatttgag gttcactttt aactatgcaa gagagacaca
    26701 cggaacagga ctatcgtgcc ctgctgattg ctgatacgcc cattattgat gttcgcgccc
    26761 ctatcgagtt tgagcaaggc gcaatgcccg ccgctatcaa tctgccgtta atgaataacg
    26821 atgaacgcgc cgccgttggc acctgctata aacagcaagg ctcagacgca gcgctggcgc
    26881 tggggcataa actggtggcg ggtgaaattc gtcagcagcg catggacgcc tggcgggcag
    26941 cgtgcctgca aaatccgcaa ggtattctct gctgcgcccg tggcggtcag cgctcacata
    27001 ttgtgcaaag ctggttgtat gcagcgggga ttgattatcc gctggtggaa ggcggttata
    27061 aggcactgcg ccagaccgcg attcaggcga ctattgaact ggcacaaaaa ccgatagtgc
    27121 tgattggcgg ttgtactggc agcggtaaaa cgctgttagt gcagcaacag ccgaacggtg
    27181 ttgatctgga agggctggcg cgccatcgcg gttcggcgtt tggtcgcacg ttacaaccac
    27241 aacttagcca ggcgagtttt gaaaacctgc tggctgccga aatgctgaaa accgacgccc
    27301 gtcaggattt gcgcctgtgg gtgctggaag acgaaagccg gatgatcggt tcgaatcacc
    27361 tgccggaatg cctgcgcgag cgaatgactc aggcggcgat tgcggtggta gaagatccgt
    27421 ttgagatccg tcttgagcgc ctgaacgaag agtatttctt gcgtatgcat catgatttta
    27481 cccacgctta tggcgacgaa cagggctggc aggaatattg cgaatacctg catcacggac
    27541 tttcggcgat taagcgtcgg ctggggttac agcgctataa cgaactcgct gcccagctgg
    27601 atacggcgct gacaacgcaa ctcaccaccg gcagcaccga cggtcatctg gcctggctgg
    27661 tgccgttact taaagaatat tacgacccaa tgtatcgcta tcagcttgag aaaaaagcgg
    27721 aaaaagttgt ctttcgcggt gagtgggcgg aagtggcgga atgggttaag gcgcggtaat
    27781 gagggacgta tttgtattag tcgcgacttg atctgacaca tggccttgaa aggttgagag
    27841 ttaccggttt tgatatgagt gtcgaatcct tatccaaaac aagaggtaac tctcatgctt
    27901 catactgcca atcccgtcat caaacacaaa gccggtttgc tcaatctggc tgaagaactc
    27961 agcaacgtat caaaagcctg taaaatcatg ggcgtttcgc gtgatacgtt ttatcgttac
    28021 cgcgaactgg tcgctgaagg cggtgtggat gcgcagatta atcgtagtcg ccgcgctcct
    28081 aaccttaaga accgtaccga tgaggcaact gaacaggctg ttgttgatta cgccgtcgct
    28141 ttcccgacac atggtcagca ccgggccagc aacgagctgc gtaaacaggg ggtttttatc
    28201 tccgacagtg gtgtccgttc tgtctggtta ctccataacc ttgagaatct caaaagacga
    28261 tactgaacaa attttatcag gtgacgttcc gtaaaaagtt gtatggagtt ctcgatgcag
    28321 tcggcgcgac tatcagtacc cccgctgaac cctggactat cgggccgccg tactggggca
    28381 tgtcaccctg gcgtgccgct ggttttccgc taatggttta tttcctcttt ctttcccggc
    28441 aagaatttat tttaatccgg aaggattatt tattttcaga tagggactca cattaaacac
    28501 gtaacatttt aattaatgca tggctgaata tgtattacac aatgtcagat atcgtaaaag
    28561 accagtgcaa ttctatgtaa actctctgac tgttcatttt atttgttgtt tcagggtcgg
    28621 aactcaccct caataaacaa ctgaatacga gtagatattc atcgtctgag ctatatggct
    28681 ttacacaata gccgacatgt cttttctcag tcaggaattt tacttttcct cactacgaag
    28741 ttgacgactt cgatatggaa tagactctta attcaagcaa ttactctgca caagcatcag
    28801 gcaatccagc tccaatattt gttataaaat aatgcgtaac attaatttca ccaacaaaaa
    28861 aaaggtttac accatcccct tgccctggta tcttaccata tttacaccac actttttttg
    28921 tatctggatc attctttgat acgattttcg ccacttgttc cctggccctc actgtcaatg
    28981 ttttcttaaa cctaccccca tcatcatttg ttagggaaaa ataagaaaaa aaataatcat
    29041 cttctacatt tgtgtatatt gatatatcca ccgctacgtg cggaaaaaaa atctgggagc
    29101 catgtaaaaa ctccatcatt ttctggtatt gccatatagt ttatgtaatg tttacttaca
    29161 agagattcat ctttgtataa ataaagataa gtaattacgc ataaa
//
LOCUS       DAZFDP010000045        28816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFDP010000045 DAZFDP010000000
VERSION     DAZFDP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..742)
                     /locus_tag="ACHJ1G_002081"
     CDS             complement(<1..742)
                     /locus_tag="ACHJ1G_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4763936.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVG"
     gene            complement(813..2927)
                     /gene="fusA"
                     /locus_tag="ACHJ1G_002082"
     CDS             complement(813..2927)
                     /gene="fusA"
                     /locus_tag="ACHJ1G_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4763937.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(3024..3494)
                     /gene="rpsG"
                     /locus_tag="ACHJ1G_002083"
     CDS             complement(3024..3494)
                     /gene="rpsG"
                     /locus_tag="ACHJ1G_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4763938.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3591..3965)
                     /gene="rpsL"
                     /locus_tag="ACHJ1G_002084"
     CDS             complement(3591..3965)
                     /gene="rpsL"
                     /locus_tag="ACHJ1G_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4763939.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(4091..4378)
                     /gene="tusB"
                     /locus_tag="ACHJ1G_002085"
     CDS             complement(4091..4378)
                     /gene="tusB"
                     /locus_tag="ACHJ1G_002085"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4763940.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4386..4745)
                     /gene="tusC"
                     /locus_tag="ACHJ1G_002086"
     CDS             complement(4386..4745)
                     /gene="tusC"
                     /locus_tag="ACHJ1G_002086"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4763941.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4745..5131)
                     /gene="tusD"
                     /locus_tag="ACHJ1G_002087"
     CDS             complement(4745..5131)
                     /gene="tusD"
                     /locus_tag="ACHJ1G_002087"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4763942.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5131..5853)
                     /gene="yheO"
                     /locus_tag="ACHJ1G_002088"
     CDS             complement(5131..5853)
                     /gene="yheO"
                     /locus_tag="ACHJ1G_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4763943.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(6020..6832)
                     /gene="fkpA"
                     /locus_tag="ACHJ1G_002089"
     CDS             complement(6020..6832)
                     /gene="fkpA"
                     /locus_tag="ACHJ1G_002089"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4763944.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            7053..7271
                     /gene="slyX"
                     /locus_tag="ACHJ1G_002090"
     CDS             7053..7271
                     /gene="slyX"
                     /locus_tag="ACHJ1G_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4763945.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7320..7910)
                     /gene="slyD"
                     /locus_tag="ACHJ1G_002091"
     CDS             complement(7320..7910)
                     /gene="slyD"
                     /locus_tag="ACHJ1G_002091"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4763946.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(8005..8205)
                     /gene="yheV"
                     /locus_tag="ACHJ1G_002092"
     CDS             complement(8005..8205)
                     /gene="yheV"
                     /locus_tag="ACHJ1G_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4763947.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8215..10020)
                     /gene="kefB"
                     /locus_tag="ACHJ1G_002093"
     CDS             complement(8215..10020)
                     /gene="kefB"
                     /locus_tag="ACHJ1G_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4763948.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(10020..10571)
                     /gene="kefG"
                     /locus_tag="ACHJ1G_002094"
     CDS             complement(10020..10571)
                     /gene="kefG"
                     /locus_tag="ACHJ1G_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4763949.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10702..12615
                     /gene="yheS"
                     /locus_tag="ACHJ1G_002095"
     CDS             10702..12615
                     /gene="yheS"
                     /locus_tag="ACHJ1G_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4763950.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12615..13637
                     /gene="yheT"
                     /locus_tag="ACHJ1G_002096"
     CDS             12615..13637
                     /gene="yheT"
                     /locus_tag="ACHJ1G_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4763951.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13631..13849
                     /gene="yheU"
                     /locus_tag="ACHJ1G_002097"
     CDS             13631..13849
                     /gene="yheU"
                     /locus_tag="ACHJ1G_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4763952.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13903..14772
                     /gene="prkB"
                     /locus_tag="ACHJ1G_002098"
     CDS             13903..14772
                     /gene="prkB"
                     /locus_tag="ACHJ1G_002098"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4763953.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14827..15231)
                     /gene="yhfA"
                     /locus_tag="ACHJ1G_002099"
     CDS             complement(14827..15231)
                     /gene="yhfA"
                     /locus_tag="ACHJ1G_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4763954.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15533..16165
                     /gene="crp"
                     /locus_tag="ACHJ1G_002100"
     CDS             15533..16165
                     /gene="crp"
                     /locus_tag="ACHJ1G_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4763955.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16216..18306
                     /gene="yhfK"
                     /locus_tag="ACHJ1G_002101"
     CDS             16216..18306
                     /gene="yhfK"
                     /locus_tag="ACHJ1G_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4763956.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18373..19593)
                     /gene="argD"
                     /locus_tag="ACHJ1G_002102"
                     /gene_synonym="dapC"
     CDS             complement(18373..19593)
                     /gene="argD"
                     /locus_tag="ACHJ1G_002102"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4763957.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19679..20242)
                     /gene="pabA"
                     /locus_tag="ACHJ1G_002103"
     CDS             complement(19679..20242)
                     /gene="pabA"
                     /locus_tag="ACHJ1G_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4763958.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20274..20876)
                     /gene="fic"
                     /locus_tag="ACHJ1G_002104"
     CDS             complement(20274..20876)
                     /gene="fic"
                     /locus_tag="ACHJ1G_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4763959.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20866..21033)
                     /gene="yhfG"
                     /locus_tag="ACHJ1G_002105"
     CDS             complement(20866..21033)
                     /gene="yhfG"
                     /locus_tag="ACHJ1G_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4763960.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21138..21710)
                     /gene="ppiA"
                     /locus_tag="ACHJ1G_002106"
                     /gene_synonym="rotA"
     CDS             complement(21138..21710)
                     /gene="ppiA"
                     /locus_tag="ACHJ1G_002106"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4763961.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21981..23162
                     /gene="tsgA"
                     /locus_tag="ACHJ1G_002107"
     CDS             21981..23162
                     /gene="tsgA"
                     /locus_tag="ACHJ1G_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4763962.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23424..25967
                     /gene="nirB"
                     /locus_tag="ACHJ1G_002108"
     CDS             23424..25967
                     /gene="nirB"
                     /locus_tag="ACHJ1G_002108"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4763963.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25964..26290
                     /gene="nirD"
                     /locus_tag="ACHJ1G_002109"
     CDS             25964..26290
                     /gene="nirD"
                     /locus_tag="ACHJ1G_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4763964.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26416..27222
                     /gene="nirC"
                     /locus_tag="ACHJ1G_002110"
     CDS             26416..27222
                     /gene="nirC"
                     /locus_tag="ACHJ1G_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4763965.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27241..28614
                     /gene="cysG"
                     /locus_tag="ACHJ1G_002111"
     CDS             27241..28614
                     /gene="cysG"
                     /locus_tag="ACHJ1G_002111"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4763966.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6957 a         7755 c         7282 g         6822 t
ORIGIN      
        1 taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca cgaccggtaa
       61 caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg aacggcttgt
      121 caatcgcacg ctctggttcc ggaatataag aatccaggaa gccagccagt tccaggattt
      181 tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga acgatcggag
      241 tgtcgtcgcc cgggaagtcg tactgagaca gaagttcacg aacttccatt tcaaccagtt
      301 ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg atgtacggaa
      361 cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg ccgtcagtcg
      421 cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg tttttaacat
      481 agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg tcatattcaa
      541 cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta tcgatctggt
      601 cgaatgcacg agcagcaccg ccgtaggttt tagccagtac ggtggtgatt gcagcggtca
      661 gagtagtttt accgtggtca acgtggccga tagtaccaac gttaacgtgc ggttttgtac
      721 gttcaaattt ttctttagac acggctatat tccttactat agtgctctcc ccttcaggag
      781 agagcacggg actttggtat taacccttag gcttatttac cacgggcttc aattacggcc
      841 tgagcaacgt tactcggcgc ttcatcatac ttcaggaatt ccatagtgta tgatgcacga
      901 cctttggtca gagaacgcag ctgagttgcg tatccgaaca tttcagacag cggtacttca
      961 gcgtggatct taacgccagt aacttcagat tcctgacctt tgagcatacc acgacgacgg
     1021 ctcaagtcac cgataacgtc accggtgttc tcttccggag tttctacttc aaccttcatg
     1081 atcggctcaa gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc gatagaagca
     1141 gccagtttaa acgccagttc agaggagtca acgtcatggt aagaaccgaa gtgcagacga
     1201 atacccatgt ctactaccgg gtagcctgcc agcggacctg ctttcagctg ttcctggata
     1261 cctttatcaa cggccgggat gtattcgcca gggattacac cacctttaat gtcgttgatg
     1321 aactcgtagc ctttcgggtt tgaacccggc tccagcgggt acatgtcgat aacaacatga
     1381 ccatactgac cacgaccacc agactgtttc gcgtgtttac cttcaacatc ggtaactttc
     1441 tggcggatag tttcacggta agcaacctgc ggtttaccta cgttcgcttc aacgttgaat
     1501 tcacgcttca tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc cgcgataatg
     1561 gtctggttag attcttcgtc agtccataca cggaaagacg ggtcttcttt agccagacgg
     1621 cccagagcca gacccatttt ttcctggtca gctttggttt tcggttcaac tgcgatggag
     1681 attaccggct cagggaattc catacgttcc agaatgatcg gcgcatccgg gtcacacagg
     1741 gtgtcaccag tggttacgtc tttcagaccg atagcagcag cgatgtcgcc cgcgcgaact
     1801 tctttgatct cttcacgttt gttagcgtgc atctgaacga tacgaccgaa acgctcacgt
     1861 gcagctttca cggagttcag tacggtatca ccagagttaa ccacaccgga gtaaacacgg
     1921 aagaaggtca ggttaccaac aaacgggtcg gtagcgattt tgaacgccag tgcagaaaac
     1981 ggctcgtcat cacttgcgtg acgctcagcc ggagtgtctt taccgtcgtc caggataccg
     2041 ttgatcgcag gtacgtcaac cggggatggc aggtaatcaa ttaccgcatc cagcatcgcc
     2101 tgaacacctt tgttcttgaa cgcagaacca caggttacca ggatgatttc gttgttcaga
     2161 acgcgctgac gcagagcacc tttgatttct gcttcagtca gttcttcacc acccaggtat
     2221 ttttccatca gctcttcaga agcttcagct gcggattcga tcaggttctg gtgccattcg
     2281 ttagccagtt caaccatgtc tgccgggata tcttcgtatt cgaaggttac gccctggtca
     2341 gcgtcgttcc agttgatagc tttcattttc accaggtcaa caacaccggt gaaatgttct
     2401 tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt tttgatctgg
     2461 ttaacaactt tcaggaagtt cgcacccatg cggtccattt tgttaacgaa cgcaatgcgc
     2521 ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg aacaccacca
     2581 actgcgcagt aaaccattac cgcaccatcg agaacacgca tggaacgttc tacttcgatt
     2641 gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc atactgctta
     2701 gccataccag accagaatgc agtagtcgca gcggaagtga tggtaatacc acgttcctgc
     2761 tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat tttatggttt
     2821 acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc gatgtgcgca
     2881 ctgataccga tattacggta gcgtgcgatg ggtgttgtac gagccatttg tttcctcgtt
     2941 tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga agcgcccgcc
     3001 tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt gttggcttcg
     3061 gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc tgcagcatca
     3121 gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg agcagcttca
     3181 acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac tggtacctga
     3241 taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac gttttcgaga
     3301 gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt ctccagcgcg
     3361 ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat atttacaaat
     3421 ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg accaatgacg
     3481 cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa ctctacgagt
     3541 ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca ttaagcctta
     3601 ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc ggagcagtca
     3661 agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg accgccacgg
     3721 atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta ggaagtcact
     3781 tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt cggtttttta
     3841 ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc ttccagcgca
     3901 ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg gttaactgtt
     3961 gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa aacgtcctca
     4021 cacaatatga ggacgccgaa ttttagggcg atgccgaaaa ggtgtcaaga aatatacaac
     4081 gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg acgaaatcag
     4141 tatagtcaat gggaatgatg tcgttcgaaa tttgaccagt caaaccgcgg gcaataaggt
     4201 cttcgttcag ggcatagacc ttaatggggg cattacgcag actttcaagg tagcggttac
     4261 cgtcaactgc ggcagttacg ccatcttgca ataatagcag ttcgtctcct tcactgagca
     4321 gacgcagcag cgcagcaaaa tccgtcagcc agggtgaacg atgtaacgtg tgaagcataa
     4381 acgcctcaaa acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa ggcatctgct
     4441 tcgagcggcg tggcttcgac aacaaagggt gtctgcggat ctaacccgcg ttcgcgcagt
     4501 gaagccgcac aaacccagca ctgttcaatg tcgtacagac ccaacaattt aaaagtggca
     4561 atgtaatcac gcgccagcac tgcatcgggc ttttgtcctg acagcagctg aaaaacgcca
     4621 tcagcaataa agaagacggc cagatcgtca gttaatgcgg aagttgccag taaagcatct
     4681 aaaccttccc gaccagcagc tgtaccatga ggtgcagtag aaaaaacaaa cgcaattcgt
     4741 ttcatcagaa ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc gcgccaagtc
     4801 cgcttaaggt aaatcccggc tgaaggtttg acgaagccag ccccagcctt cctgcttcag
     4861 tttcatcgac gataccgcgg cgtaacgctg ccgctacgca gatattcagc gccacaccat
     4921 gttgcgcatt caactgttgc cagccgcgca caaggtcaaa ttcgtcactt gccggtgagg
     4981 tcaattggtt agcgttatag accccttccc gatagaaaaa gacgctgctt aactcatggc
     5041 cttctactat cagcgcctgc gcaaactgaa aagcactact cgcctgttgc gtaccgtatg
     5101 ctggcccggt caccacgatg gcaaaacgca ttacttatct tgcccctgga aatcaccgct
     5161 tttgaactgg cggatgtaga gatagacagt gtgtttggag atgttcaggc ggtcagcaac
     5221 ctggttgatc gcgtctttaa tatcgaagat ccctttctcg tagagattca gcacgatctg
     5281 acgatttttg gcgttattag aaacattgcg atcggcattc acttcttcga tggtgaactc
     5341 cagcgtttgg gtaaccagat cttcaacaga agaggcaaag ttgacgcttg aaccgacatc
     5401 cggggtttct ggcggcacaa aagtgctcat aatctgcgag aagggaacat caagattcat
     5461 attgatgcac agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga tagtcaggga
     5521 cttcattaat acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc tgctatccgc
     5581 tcccgtcata tcgtgcagca tacgtagcgc caggtcagta attggcgaac caatcttccg
     5641 gcctgtatgt tcaccgttag caatgcgaat ggctgaacat tttagatcct gcaaagagtg
     5701 caaaacgatt tcacagtggg agccaataag catcgctaac ccgtccacca ccgcttcgta
     5761 ggatttcaga atatcaaaat cggtctggtc gaaaggacgt tgatccagta aatccaactc
     5821 actggtttcg ttggttaaaa gcgacctgga catgaaaaaa aacactcctt ttcaggagcc
     5881 tgtcgttaac ttttcagggc aggctcatta atgatgcggg taactaaatt aatacagcgg
     5941 aggttccgct ttccagcact aattatatcc ggcctgtaat aaaaaaaccg ccgcctggtc
     6001 aggcggcggt tcttaatgct tattttttag cagaatctgc ggctttcgca tcagcttccg
     6061 gctttgcatc agccttcggc gctggtttca catccagcag ctctacgtca aacaccaggg
     6121 tagaattcgg tgggatcccc ggaacacccg ctttgccgta agccagttct ggtggaataa
     6181 ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccaa cccgggataa
     6241 caccgtccag acggaaagaa agcggttcac cacgggtgta agagttgtcg aactctttac
     6301 cgtcgatcag cgtacctttg tagttcacta caacagtatc gctgtctttc ggtgcttcgc
     6361 ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc acacctttct
     6421 ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc gcgtcttttt
     6481 ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga gtctgttcga
     6541 tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca gcgatcagct
     6601 gatctttatc cagtttgatg cccagttttt cttgttcttt aagagagttt tccatgtaac
     6661 gacccagcga agcacccagt gcataagctg atttctgatc gtcatttttg aacgctgctt
     6721 tgctgtcagc agttgtagca ggttttgcag cttcagcagc aaaagtgatt ggtgcatgca
     6781 gggcaacggc cattgtggtc gccagcagcg ttactttaaa cagtgatttc atccatatct
     6841 ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact ataaagcgtt
     6901 gtcgaacaaa tctacataca gacacgccct attatcatct attttcagac tctttttgtt
     6961 taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt taagtagaat
     7021 ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg gaagcacgcc
     7081 tggcagagct ggagagccga ctggcttttc aggaaatcac cattgaagaa ctgaacgtca
     7141 cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt ctgctgaccg
     7201 agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa acgccaccgc
     7261 cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcacc ttttcggtat
     7321 tagtggcaac cacaaccgcc gttgccttta ccgccacagc agccttcgcc accgtgttca
     7381 tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc gtggtgatga
     7441 tcgtgcgcgc cgtgaacgtg gccatgagcc agttcttctt cggtcgcttc gcgaatcgcc
     7501 acaacttcaa cgttgaattt caggttctga ccggccagca tgtggttacc atcaaccacg
     7561 acgtgatcgt cttcaaccgc agtgatttca accggtaccg gcccctggtc ggtttcagcc
     7621 aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt aggaacacgt
     7681 tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc gacatcaaat
     7741 ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag ggaaccgtga
     7801 ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac accgtcttct
     7861 gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat gatatctcct
     7921 gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg tactcactag
     7981 cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca cttgctcatc
     8041 tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc cgcacttaac
     8101 acattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct gcgcctgaca
     8161 ggccgggcat tttgcgcccg cgataaaacg ttttcggatt gccatcttta ccctctactc
     8221 aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct ggaaaatctc
     8281 ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcag catgcatcgg
     8341 gattaactcc cgcagcattc gcatatccag gcggcgaaaa tgcagttgcg cccgctgtgc
     8401 ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta acgcactgga
     8461 gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg cttccacacg
     8521 tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata tttccaccag
     8581 cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg cctctgcacc
     8641 cgcagaacgt aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt agccatattt
     8701 acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca ttttatttgc
     8761 catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa tgacctgggg
     8821 tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa actggcggga
     8881 tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa gcgtcaccgt
     8941 caccagcaac agcgccatct ggtcgccctg gaataaacgt tgtgaagaag cggtagaaaa
     9001 gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca tccgctctga
     9061 gctacgcacg ccatacaatc gcgccagcag atacagcacg agaattttca ccgccaccag
     9121 cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga ggttgagcga
     9181 catgccgaca gagataaaga acaaaccgag cagcaatcct ttgaagggat cgatagccgt
     9241 ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa atgtaccgag
     9301 cgccatcgac agccccagcg catccataaa caatgcggaa cccaacacca gcagcagcgt
     9361 cgcggcggtg aacacttccc gcacgccaga agctgcaata aagcggaata ccggacgcag
     9421 taaatagcgc ccaccaatca gcatgccgac aaacgccagc accttcatgc cgatcttcat
     9481 ccagtcgaaa tgttcgtctg ccgaacccgc caacaacggc actaacgcca gtgccgggat
     9541 caccgccaga tcctgaaaca gcagtaccga aaacccgagc tgcccggatt cgctgcgatt
     9601 catccctttc tcacgcatca attgcaacgc cattgcggtc gaagacatcg caaggccaat
     9661 gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc ccgccagcaa
     9721 cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgaacgcc gcagttgcca
     9781 aagcttggag ggattcaact caaggccgat gataaacatc aggaatacca cgccgagttc
     9841 cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccacg ggccaattgc
     9901 aatccctgcc agcaaatatc ccaacacagc gccaatcccc agccgcgatg ccaacggcac
     9961 cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg aaccttccat
    10021 cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct ggcgtggctc
    10081 gccagctcct gtgcgctttg ccgtctcgcc cagtaaataa tgatgggact taaccaatgc
    10141 atccggcaca tgcccgccgc cagttcaaag ggacgcagaa cgtcgctcat ggggtagcga
    10201 ttcaatgcgt cataacggta agcactttcc ggctcgccgg tggtgatcac gctacgccag
    10261 tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacggct taatacccgg
    10321 tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg ctgaaagaca
    10381 atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa aaaatcggga
    10441 tagtgcgcat aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg tttaagcagt
    10501 acccggtttg ccaccgagtc ctgagattcc ggatgggcaa acagcagcaa aactttcgct
    10561 ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt tttgggctac
    10621 cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat aagttaacat
    10681 agtctgaaca tacggcacct tatgattgtt ttctcctcgt tacaaattcg tcgcggcgtg
    10741 cgcgtcctgc tggataatgc caccgccacc atcaaccctg ggcagaaagt cggcctggtg
    10801 ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga aatcagcgcc
    10861 gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa tcaggaaacg
    10921 ccggcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga atatcgtcaa
    10981 ctagaagcgc agctacacga cgccaacgaa cgtaacgacg ggcacgccat tgcgaccatt
    11041 catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc cagcctgctg
    11101 cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt ctccggtggc
    11161 tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt gctgctgctc
    11221 gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa atggctgaag
    11281 agctatcagg gcacgctgat cctgatctct cacgaccgcg acttcctcga tccgatcgtc
    11341 gataaaatta ttcatatcga acaacaaagc atgttcgagt acaccggcaa ctacagttcg
    11401 tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtacga aagccagcag
    11461 gaacgcgtag cgcatctgca aagttatatc gaccgtttcc gtgccaaagc caccaaagcg
    11521 aagcaggccc agagccgcat taagatgctc gagcgtatgg agctaattgc ccccgcgcac
    11581 gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa tccgttactg
    11641 aagatggaaa aagtcagcgc gggctatggc gatcgcatta ttctcgactc gattaaactg
    11701 aacctggtgc ccggctcgcg cattggtctg ttaggccgca atggcgcggg taaatcgaca
    11761 ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg tctggcgaaa
    11821 gggatcaaac tcggctactt cgcccagcat caacttgaat acctgcgcgc cgacgaatca
    11881 cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact gcgtgactac
    11941 ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg cttctccggt
    12001 ggggaaaaag cccgcctggt gctggcatta attgtctggc agcggccgaa tctgctgctg
    12061 ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac cgaagcatta
    12121 atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct gcgttccacc
    12181 actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg cgatctggaa
    12241 gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga cgaagcgcca
    12301 aaagagaatg cgaacagcgc ccaggcacgt aaagatcaga agcgtcggga agctgagctg
    12361 cgtgcgcaaa cccagccact gcgtaaagag attgcccgtc tggaaaaaga gatggagaag
    12421 ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact gtatgaccag
    12481 agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa atccggcctg
    12541 gaagagtgcg aaatggcatg gctggaagcc caggagcagc ttgagcagat gctgctggaa
    12601 ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattc agcagcagtg
    12661 ctgaattcat ccccatgcgc ggctttagca attgtcatct gcaaaccatg ctgccgcgtc
    12721 tgtttcgtcg tcaggtgaaa ttcaccccgt actggcagcg gctggagttg cccgacggcg
    12781 attttgtcga tcttgcgtgg agtgaaaacc ctgcacaggc gcaacataaa cctcgtctgg
    12841 tggtgtttca cgggctggaa ggcagtctca acagccctta cgcccacggt ctggttgagg
    12901 cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc agcggtgaac
    12961 caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt tggtttttac
    13021 gctggctgca acgcgaattt ggtcatgcgc caacggctgc cgtcggctat tcgctcggcg
    13081 gtaatatgct ggcctgtttg ctggcaaagg aaggcaatga tctcccggtt gatgcggcgg
    13141 tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa aagggctttt
    13201 cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgcg cgcaagctgg
    13261 cagcctaccc cggaacgctg ccgattaatc tcgcgcagtt aaaatcggta cgtcgcatcc
    13321 gtgaatttga cgatctcatc accgccagaa ttcacggcta cgccgacgct atcgactatt
    13381 atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg ctgattattc
    13441 acgccaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa agtctccccc
    13501 cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt ggcggtacat
    13561 tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca acgtatctgg
    13621 aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg aaaatttaat
    13681 tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta cacttgaaca
    13741 gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg tatggtcgga
    13801 gctgcacgaa acggtcaata ttatgccgcg cagtcagttt cgcgaataac ctgcaaccgt
    13861 cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc caaacatccg
    13921 gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct cgcgtttcgt
    13981 aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag ttttcaccgt
    14041 tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg gcggcatatc
    14101 agctacttcg gccccgaggc taacgacttc ggcctgctgg aacaaacctt cattgaatac
    14161 ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgacga agccgtaccg
    14221 tggaatcagg taccggggac attcaccccc tggcaacctt taccggaacc cactgatgta
    14281 ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt tgcgcagcat
    14341 gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca aaaactgatc
    14401 cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcagt agtgcgttca
    14461 atggaagact atatcaacta catcacaccg cagttttccc gcacccatct taacttccag
    14521 cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc gtcgctcgat
    14581 gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc ctggctgctg
    14641 gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtacc gggcggcaaa
    14701 atgggtctgg caatggaatt aattatgctg ccgctggtgc aacgattgat ggaaggaaag
    14761 aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca caacgggata
    14821 gttaagtcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt tttccagcat
    14881 caacgccact gaacaatatt tctcggcaga gagatcaacc gcacgcgcaa ccgctgcgtc
    14941 tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca ggcgtggtgc
    15001 ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct gacgcccttt
    15061 ttgcaggatc gaaaccacat cgatggcact gcaaccaccc gccgccatca acaccatttc
    15121 catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctgat gaccagaggc
    15181 ggattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca tttttgctac
    15241 tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc aacggaaggc
    15301 gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta tgctaaaaca
    15361 gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg tcacattacc
    15421 gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg caatccagag
    15481 acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc gcatggtgct
    15541 tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc acattcataa
    15601 gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc tgtactacat
    15661 cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa tgatcctctc
    15721 ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg gccaggaacg
    15781 tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt acaaaaaatt
    15841 tcgccaattg attcaggtaa acccggacat tctgatgcgt ttgtctgcac agatggcgcg
    15901 tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg tgacgggccg
    15961 cattgcacag actctgctga atctggcaaa acaaccagac gctatgactc acccggacgg
    16021 tatgcaaatc aaaattaccc gtcaggaaat tggtcagatt gtcggctgtt ctcgtgaaac
    16081 cgtgggacgc attctgaaga tgctggaaga tcagaacctg atctccgcac acggtaaaac
    16141 catcgtcgtt tacggcactc gttaatcccg tcggagtggc gcgttacctg gtagcgcgcc
    16201 attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa ctatgcactt
    16261 cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg cgaattacga
    16321 ttcggtctgc tcttctctct cgttcctgcc tgttgcaata ttgcgggcct tgatacgcct
    16381 cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac ctgtagctta
    16441 ctgacacagc tgctactggc aaaagatgtt cccctgccct ttttgctgac cggattaacg
    16501 ctggtacttg gcgtcactgc tgagttgggg ccattgcacg caaaattgct tcccgcatcg
    16561 ctgctggcag ccatttttac cctcagtctg gcgggataca tgccggtctg ggaaccgttg
    16621 ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg gttctggatc
    16681 tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgtgaact ggcagattat
    16741 tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc gctgccgccg
    16801 ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca gcaaatgcat
    16861 atgctttccg cgcaaaataa tactgactac aagcggatgc tgcgtatttt ccaggaggcg
    16921 ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt gcaaaagctg
    16981 gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt cgccgctcgc
    17041 ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacgcgttt tacgatggaa
    17101 aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc ggttgggcaa
    17161 ttctgctact ggcatttcag ccgcatcgcc cgcgtgctgc gcacccaaaa accgctctat
    17221 gcacgtgact tactggccga taaacagcgg cgaatgccat tacttccggc gctgaaaagt
    17281 tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt gatgttaagc
    17341 gttgccagcc tgatgggcac cgcgctgcat ctgccgaagt cgtactggat cctgatgacg
    17401 gtattgctgg tgacacaaaa tggctatggc gcaacccgtc tgaggattgt gaatcgctcc
    17461 gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa aattcccgaa
    17521 ggttacaccc tgacgttgat gctgattacc accctcgcca gctacctgat attgcgcaaa
    17581 aactacggct gggcgacggt cggttttact attaccgcag tgtataccct gcaactattg
    17641 tggttgaacg gcgagcaata catccttccg cgtcttatcg ataccattat tggttgttta
    17701 attgctttcg gcggtactgt ctggctgtgg ccgcagtggc agagcgggtt attgcgtaaa
    17761 aacgcccatg acgctttaga agcctatcag gaagcgattc gcttgattct tagcgaggat
    17821 ccgcaaccta cgccactggc ctggcagcga atgcgggtaa atcaggcaca taacactctg
    17881 tataactcat tgaatcaggc gatgcaggaa ccggcgttta acagccatta tctggcagat
    17941 atgaaactgt gggtaacgca cagccagttt attgttgagc atattaatgc catgaccacg
    18001 ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt acagtcttgt
    18061 gaaatcgcca ttcagcgttg tcagcagcga ctggagtatg acgaaccggg tagttctggc
    18121 gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc aggtacgctg
    18181 gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat ttcgtcgatg
    18241 gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg ggattcgaag
    18301 gcgtaatgca ggctaaacca tcggataagg cattcacgcc gcatccgaca tcttttgcct
    18361 gatgcttcgt atttacgccc caaccacctt cgccaccgcg tgggcgaaac gttgcatccc
    18421 ttcatcgata tccgcatctt ccaccaccag cgacggtgca aaacgcatca catccggtcc
    18481 ggcattcagc accattacgc cagcctctgc gcccgcatac aggaaatcac gcgcccgacc
    18541 tttgtactgt ggtttcagct ctgcgccaat caacagcccc ataccgcgaa tatcgctaaa
    18601 cacatcgtac tgctgatcga tcttctgcag atggtcaaca aaacgctggc gtttcgcctg
    18661 aatgccttcc agcacttcag gggtattgat gatatcaaac gccgcccccg ctactgcaca
    18721 ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg cagaagcaat
    18781 ttctgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt tcgcagaggt
    18841 cagaatatcc ggcgtcacgc cgtagtgcat gtaagcaaac aaatcgccgg tccgccccat
    18901 cccgcactgt acttcatcaa acaccaataa tgcctgatgt tgatcgcaca gctcgcgcaa
    18961 gccctgcaaa aactctggcg tcgctgccgt cacaccgccc tcgccctgga tcggctcaac
    19021 caccaccgca caggtgtgat catccatcac cgctttcact gcgtggagat cgttaaaagg
    19081 aacgtggatg atgtctgccg gttttggccc aaagccgtcg gaatattttg gctgcccgcc
    19141 caccgagacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa taattttggt
    19201 tttgaacgga ctatgacgca cacaggcgta atggcgtgcc agtttaaagg cggtttcgtt
    19261 agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgcct caatcagttt
    19321 gcgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc acagagtttc
    19381 gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg ccgtaactgc
    19441 aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc ggctgccctg
    19501 accttttacc ggaatgaact ctgccggagc ataaatcggc aggatcactt catcgaaagt
    19561 cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt acgcataaat
    19621 aaatgtgaaa ttataatcac aaaatatgca taaaaaatca ttgaatggca atcagaaatc
    19681 agcgatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata ctttctggat
    19741 ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc tcgcgggttt
    19801 cgctccaggc cgtcacgtca aagcatgctg gtaatgagcc aggttccacc accagcgaat
    19861 ggtagcgtgt cacggtaagt ggatttgcca gcccccgaaa tacgccctca ccgttatgtg
    19921 taatcggcga ggttttgccg tgcatgacct ttgcggcgcg cacaactttg ccgccaaatg
    19981 cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc ccggcatagt
    20041 ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggccg ggtgatatga
    20101 caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg ttgcgcttaa
    20161 ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta aaagaatcgt
    20221 agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcgct attttactca
    20281 gattccccgg cttcgcttac cactttgcta aatatcatct gcagcgcggt gagatccccc
    20341 attgccccac tctgatttgc ctgattccag gcctcttttt cgataccctg ccagctcagt
    20401 tgatacccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag accacttccc
    20461 acccggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg ctcgacgaac
    20521 ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat cagggcattg
    20581 ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata aatatcaact
    20641 tcacgcagtt gccccgccca gtcgaaaata tcctgataca gctggcgatg gatagtgcgc
    20701 aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg cagcgccgtc
    20761 atttcgtaag cggcctgttc cagccgctgc tgctggtgga tgttcagccg gttacgcatg
    20821 atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc gctcatagtg
    20881 cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga ctaaaggcat
    20941 ctcgacgcct tcaagacggc gactggcctg gaaattacga ttacgctgaa gctcccagag
    21001 acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac gtgtccattt
    21061 gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc gggcaagatt
    21121 gcccgcgcga aaaatcatta cggcaggact ttagcggaaa ggataactac cggttttgac
    21181 ggcacattct ggtacggacc aacgtcatga gtcggcacct gggaaatctt atcggcaacg
    21241 tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg accatggtca
    21301 aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc tttgtcagcg
    21361 gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc ttcatttttg
    21421 attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc ctgaatcata
    21481 aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt cacataatcg
    21541 acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc gatgttacca
    21601 gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc gggagaaaga
    21661 gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat gagatttcct
    21721 taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc aagaacaagg
    21781 ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat cactttttat
    21841 tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat aaaacattta
    21901 aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta tgccatttta
    21961 tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca tggattagct
    22021 ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg atgggaaata
    22081 tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc ttcctcaacg
    22141 ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa
    22201 cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg atgttcagcc
    22261 acagcctggc gctgttctcg gcggcgatgt tcattctcgg ggtggtcagc ggcatcacca
    22321 tgtcgattgg tacattcctg gtaacacaaa tgtatgaagg gcgtcagcgc ggttcccgcc
    22381 tgttatttac cgactccttc ttcagtatgg ctgggatgat tttcccaatg atcgccgcgt
    22441 ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg ctggtgtatg
    22501 tcgctatttt tattctgacc ttcggctgtg agttcccggc gctgggcaaa catgcgccaa
    22561 aaacggatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt ctctctgttg
    22621 cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct gagtatgcca
    22681 aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc tggatgtcat
    22741 acatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg caacgcattc
    22801 tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc ggaacaccag
    22861 cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc tataccacca
    22921 tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt aactttgtcc
    22981 tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg attgttgaac
    23041 atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc gtctttgtga
    23101 tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg acctctcatt
    23161 aattgctcat gccggacggc actctcgtcg tccggccttt tcctctcttc ccccgctacg
    23221 tgcatctatt tctataaacc cgttcatttt gtccattttt tgcacaaaca tgaaatgtca
    23281 gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt aaggagtata
    23341 taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag gtaggcggta
    23401 atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc ggtaacggta
    23461 tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc aactttgata
    23521 ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc tcgtcttact
    23581 tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac gagaaacacg
    23641 gcatcaaagt tctggtcggc gaacgcgcta tcaccatcaa ccgtcaggag aaggtgattc
    23701 actccagcgc cgggcgcacc gttttttatg acaagctgat catggcgacc ggttcctacc
    23761 cgtggatccc gccaatcaaa ggttctgata ctcaggactg ctttgtctat cgcactattg
    23821 aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa acgcggtgcc gttgttggtg
    23881 gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggtatt gaaacccacg
    23941 ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt ggcgagcagc
    24001 tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac acccttgaga
    24061 ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc agcgaactgg
    24121 aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg gcaacccagt
    24181 gtggtctgga cgttgctccg cgtgggggta tcgtcattaa tgattcctgc cagacttccg
    24241 atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta tttggtctgg
    24301 tagcacctgg ctacaaaatg gcgcaggtcg ccgttgacca tattctcggt agcgaaaacg
    24361 cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac gtaggcggta
    24421 ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta cgtttacctc gacgaaagta
    24481 aagagatcta caaacgcctg attgtcagcg aagacaacaa aaccctgctc ggtgcggtac
    24541 tggtgggcga taccagcgac tacggcaacc tgctgcaact ggtgctgaat gctatcgagc
    24601 tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt aagccgtcta
    24661 tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac gtcaccaaag
    24721 gtgatctgat tgctgccatc aacaaaggct gccacaccgt tgcggcgctg aaagctgaaa
    24781 ccaaagcggg tactggctgc ggtggctgta tcccgctggt tactcaggta ctgaacgcgg
    24841 aactggcgaa acagggcatc gaagttaaca acaacctgtg cgaacacttt gcttattcgc
    24901 gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa gaactgctgg
    24961 cgaaacacgg caaaggctac ggttgtgaag tttgtaaacc gaccgtcggt tcgctgctgg
    25021 cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag gattctaacg
    25081 acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg cgttctccgg
    25141 gcggtgaaat caccccggaa gggctgatgg cagtgggtcg tatcgcgcgt gaatttaatc
    25201 tctacaccaa aatcaccggc tcccagcgcc tggcgatgtt tggcgcacag aaagacgatc
    25261 tgccggaaat ctggcgtcag ctgattgaag ctggcttcga aaccggtcat gcctatgcga
    25321 aagcactgcg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctac ggtgttggcg
    25381 atagcgtcgg cctcggcgtg gaactggaaa accgctacaa aggcatccgt acgccgcaca
    25441 aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag ggtaaagacg
    25501 tgggtattat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac ggcggcatga
    25561 aaccgcgtca tgcggatctg ctggcagcgg atatcgatcg cgaaacgctg atcaaatatc
    25621 tcgaccgctt catgatgttc tacatccgta ccgccgacaa actgacgcgt accgcgccgt
    25681 ggttagaaaa cctcgaaggc ggcatcgatt acctgaaagc ggtgatcatt gacgacaaac
    25741 tggggctgaa cgctcatctg gaagaagaga tggcgcgcct gcgtgaagcg gtagtgtgtg
    25801 agtggactga aacggtcaat acaccgtccg cgcagactcg cttcaaacac ttcatcaaca
    25861 gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac cgtccggcaa
    25921 cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgagcc agtggaaaga
    25981 catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt taggcgacga
    26041 gcaggtcgcg attttccgcc cgtatcacag cgaccaggtg tttgcgatca gcaacatcga
    26101 tccgttcttc gagtccagcg tgctgtcacg cgggctgatt gcggaacacc agggcgagct
    26161 gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agtgatggct tgtgcatgga
    26221 agacgaacag ttttccgtca aacattacga agcacgagtg aaagacggcg tggtgcagct
    26281 gcgcggttaa tgttttaacg ggaggcgcaa tgcctcccct ttttgcatgg tcctgtaatg
    26341 accttcggta taatgcagga cattttttaa actttttgtt ttattttttg tttttatttt
    26401 ttaaaggata atcaaatgtt tacagacact attaataagt gtgcggctaa tgctgcacgc
    26461 attgcacgcc tgtcggcaaa taacccgctc ggcttttggg tcagctccgc catggcgggc
    26521 gcatatgtgg gtcttgggat catcctgatt ttcacgctca gtaatttgct cgacccgtcc
    26581 gtacgtccgc tggtgatggg cgcgaccttt ggtatcgcct taacgctggt gattatcgcc
    26641 ggttctgaac tgttcaccgg acacaccatg ttcctcacct ttggggtaaa agcgggcagc
    26701 atcagccacg ggcaaatgtg ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt
    26761 tccgtcttcg ttgccatgct ctatagctgg ggcggcggta gcctgctgcc ggtagatacc
    26821 agcatcgttc actccgtcgc gctggctaaa accactgcac cggcaatggt actgttcttc
    26881 aaaggtgcat tgtgtaactg gctggtttgc ctggcaatct ggatggcgct gcgcactgaa
    26941 ggggcggcga aatttatcgc tatctggtgg tgtctgctgg catttatcgc gtccggctac
    27001 gagcactcta tcgcaaacat gacgctgttc gcgctctcct ggttcggcaa ccacagcgaa
    27061 gcctacacgc tggcgggtat tggtcataac ctgctgtggg tgacgctggg taatacttta
    27121 tcaggtgccg tattcatggg attgggttat tggtatgcta cgccgaaagc gaatcgtccg
    27181 gttgcggaca aatttaatca aactgaaacg gctgccggtt aattactaag gggtttttac
    27241 gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt
    27301 ggtgatgtcg cggaacgcaa agcaaggttg ctgttagacg caggcgctcg cttaacggtg
    27361 aatgcattag cgtttattcc acagttcacc gcatgggcag atgcaggcat gttaaccctc
    27421 gtcgaagggc catttgatga aagccttctc gacacctgct ggctggcgat tgcagcgacg
    27481 gatgatgaca cgcttaacca gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt
    27541 aacgtagtcg atgcgccgaa agccgccagc tttattatgc cgtcgattat tgaccgctca
    27601 ccgctaatgg tagcggtctc ctctggcggc acctctccgg ttctggcgcg cctgttgcgc
    27661 gaaaaactcg aatcactgct gccgttgcat ctgggccagg tggcgaaata tgccgggcaa
    27721 ttacgcgggc gagtgaaaca acagttcgcc acgatgagtg agcgtcgccg tttctgggag
    27781 aaattgttcg ttaacgaccg cctggcgcag tcgctggcaa acaacgatca gaaagccatt
    27841 actgaaacga ccgaacagtt aatcaacgaa ccgctcgacc atcgcggtga agtggtgctg
    27901 gttggtgcag gtccgggcga tgccgggctg ctgacgctga aaggactgca acaaattcag
    27961 caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa tctggtacgc
    28021 cgcgatgctg atcgcgtttt cgtcggcaaa cgcgcgggat accactgtgt accgcaggaa
    28081 gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa
    28141 ggtggcgatc cgtttatttt tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg
    28201 ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc tgcctattcg
    28261 ggtattccgc tcacgcatcg cgattatgcc cagagcgtgc gcttaattac cggacactta
    28321 aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag aaaaacagac gctggtgttc
    28381 tatatggggc tgaatcaggc tgcgactatt cagcaaaagc tgattgaaca cggtatgcct
    28441 ggcgaaatgc cggtggcaat tgtcgaaaac ggaacagcag tcacgcagcg cgtgattgac
    28501 ggtacgctca cgcaactcgg tgaacttgct cagcaaatga acagtccatc gctaattatt
    28561 attggtcggg ttgttggcct gcgcgataaa ctgaactggt tctccaacca ttaatttaac
    28621 ccgggccaga gaattctggc cttcttaatg actcttttta tatattcagc aaataaaaca
    28681 tagcccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28741 aattaagtca ttcgctcact gacgccaatt aaatattcct gtatcgttct taacgagcag
    28801 tctgcttata ttcata
//
LOCUS       DAZFDP010000046        28812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFDP010000046 DAZFDP010000000
VERSION     DAZFDP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..941
                     /locus_tag="ACHJ1G_002112"
     CDS             87..941
                     /locus_tag="ACHJ1G_002112"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO4763967.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            977..1867
                     /locus_tag="ACHJ1G_002113"
     CDS             977..1867
                     /locus_tag="ACHJ1G_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4763968.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1928..3427
                     /locus_tag="ACHJ1G_002114"
     CDS             1928..3427
                     /locus_tag="ACHJ1G_002114"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4763969.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3428..4417
                     /locus_tag="ACHJ1G_002115"
     CDS             3428..4417
                     /locus_tag="ACHJ1G_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4763970.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            4439..5401
                     /locus_tag="ACHJ1G_002116"
     CDS             4439..5401
                     /locus_tag="ACHJ1G_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4763971.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            5398..6444
                     /locus_tag="ACHJ1G_002117"
     CDS             5398..6444
                     /locus_tag="ACHJ1G_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO4763972.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(6536..7885)
                     /gene="accC"
                     /locus_tag="ACHJ1G_002118"
     CDS             complement(6536..7885)
                     /gene="accC"
                     /locus_tag="ACHJ1G_002118"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4763973.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7896..8366)
                     /gene="accB"
                     /locus_tag="ACHJ1G_002119"
     CDS             complement(7896..8366)
                     /gene="accB"
                     /locus_tag="ACHJ1G_002119"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4763974.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9232..10062)
                     /locus_tag="ACHJ1G_002120"
     CDS             complement(9232..10062)
                     /locus_tag="ACHJ1G_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4763975.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(10099..11073)
                     /locus_tag="ACHJ1G_002121"
     CDS             complement(10099..11073)
                     /locus_tag="ACHJ1G_002121"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4763976.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11225..13165
                     /gene="csrD"
                     /locus_tag="ACHJ1G_002122"
     CDS             11225..13165
                     /gene="csrD"
                     /locus_tag="ACHJ1G_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4763977.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13470..14513
                     /gene="mreB"
                     /locus_tag="ACHJ1G_002123"
     CDS             13470..14513
                     /gene="mreB"
                     /locus_tag="ACHJ1G_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4763978.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14579..15682
                     /gene="mreC"
                     /locus_tag="ACHJ1G_002124"
     CDS             14579..15682
                     /gene="mreC"
                     /locus_tag="ACHJ1G_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4763979.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15682..16170
                     /gene="mreD"
                     /locus_tag="ACHJ1G_002125"
     CDS             15682..16170
                     /gene="mreD"
                     /locus_tag="ACHJ1G_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4763980.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            16179..16772
                     /gene="yhdE"
                     /locus_tag="ACHJ1G_002126"
     CDS             16179..16772
                     /gene="yhdE"
                     /locus_tag="ACHJ1G_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4763981.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16762..18231
                     /gene="rng"
                     /locus_tag="ACHJ1G_002127"
     CDS             16762..18231
                     /gene="rng"
                     /locus_tag="ACHJ1G_002127"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4763982.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18299..22099
                     /gene="yhdP"
                     /locus_tag="ACHJ1G_002128"
     CDS             18299..22099
                     /gene="yhdP"
                     /locus_tag="ACHJ1G_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4763983.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22255..23700
                     /gene="tldD"
                     /locus_tag="ACHJ1G_002129"
     CDS             22255..23700
                     /gene="tldD"
                     /locus_tag="ACHJ1G_002129"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4763984.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23828..24757)
                     /gene="aaeR"
                     /locus_tag="ACHJ1G_002130"
     CDS             complement(23828..24757)
                     /gene="aaeR"
                     /locus_tag="ACHJ1G_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4763985.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24940..25143
                     /gene="aaeX"
                     /locus_tag="ACHJ1G_002131"
     CDS             24940..25143
                     /gene="aaeX"
                     /locus_tag="ACHJ1G_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4763986.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            25151..26083
                     /gene="aaeA"
                     /locus_tag="ACHJ1G_002132"
     CDS             25151..26083
                     /gene="aaeA"
                     /locus_tag="ACHJ1G_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4763987.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            26089..28056
                     /gene="aaeB"
                     /locus_tag="ACHJ1G_002133"
     CDS             26089..28056
                     /gene="aaeB"
                     /locus_tag="ACHJ1G_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4763988.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            28148..28420
                     /locus_tag="ACHJ1G_002134"
     CDS             28148..28420
                     /locus_tag="ACHJ1G_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4763989.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28476..28739)
                     /gene="yhcN"
                     /locus_tag="ACHJ1G_002135"
     CDS             complement(28476..28739)
                     /gene="yhcN"
                     /locus_tag="ACHJ1G_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4763990.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6958 a         6872 c         7740 g         7242 t
ORIGIN      
        1 tttgctgtgc tagtttgctt tcaaaatgaa agtgaatatt ctctttcttc attttgtaag
       61 gcgaacgaca aataacagga gctgcgatgt acatcatttc aagcaaaaac atgctgaaaa
      121 aagcccagtc agagggatat gccgtccccg ctttcaacat tcataacctt gaaactttac
      181 aggtagttgt tgagagcgct gcacaacttc gctctcccgt tatgctggcg ggaacacccg
      241 gtacttatcg ttatggtggc gtgggaagtt taatttcgtt agtacaatcg ctggctcgcg
      301 agtacaacct gccgttagtc ctgcatctcg accatcatga agagagtgat gacattttta
      361 acaaggttcg cgcagggatc cgttctgtca tgattgatgg ctcccatttg ccatttgaag
      421 acaacattgc gcgagtgcaa gaagttacca ccttttgcca tcgctttgat gttagcgttg
      481 aggccgagct agggcgtctt ggcggccagg aggacgatct gctggtggat gaaaaagatt
      541 ccgcttatac ctcaccagcc gcagctgcag agtttgttca acgtacccaa attgactccc
      601 tggccgtagc aatcggcaca gcacatggtt tatatgcgca tgaaccacga ctggattttg
      661 tgcggctgga acagattcgt cagcgagtgg agattccact ggtgttgcat ggtgcttcgg
      721 gcttatcaga acaggacgtt cagcactgta ttcgccgcgg tatttgtaaa gtgaatgtcg
      781 cgacagaatt gaaaattgcg tttgccgatg cgttaaaagg atatttctac aacaatcctg
      841 cggcaaatga tccacgccac tatatgcagc ccgcaaaggc cgccatgaaa gaagtcgtaa
      901 ctcgtattat tggtgtctgt ggtagtgaag gaaaaattta agacgttgta ccctacaaaa
      961 acaatgagga ttgtttatga aaaatattaa attacttggt tcagctatgt tggtgagcct
     1021 ggcattgttc tcgcaaaatt cgttggcaaa agagtataaa attggtgcgt cattattaac
     1081 acagcaacat cctttctata ttgatcttgc gaatgccatg aaagaagagg caaaaaaaga
     1141 caatgtcaat ttaagtgtct ctattgccaa tcaggattta aacaaacaac tttctgatgt
     1201 tgaggatttt attactaaaa aagtggatgc cattattatc tctccggtcg attcgaaagg
     1261 cgtccaggcg gcgataatta aagccgataa ggccggtatt cccgttatca ccgtcgacgt
     1321 cgctgcggaa ggcgttccgg tcgtatcgca tgttgcgacc gataactatg cgggaggcgt
     1381 tgaagcaggc aagttaatgg gcaaactttt gaatggtaaa ggtactgtag ccattatttc
     1441 ttatccggca ctacaatcgg tggttgcgcg cgttgatggt tttaagaaag ggttaagcga
     1501 tacaccggat attaaaattg ttgctgaaca gcctggtatt acgcgggctg aagcgttaac
     1561 gactgcgcaa aacattatgc aggcaaatgc caacctgaat ggtctgtttg gctttggtga
     1621 tgatgcggcg ctggcagcgg ttatcgcagc aaaatctgcg cataacgaca acatcaaaat
     1681 cattggtttt gacggcatga aagaagcgcg tgatgccgtt gacagtgaaa aaacgtttgc
     1741 ggccgtgatt cggcaatatc ccgatcaaat gggtgctaaa gccattgatg ccgctgttga
     1801 tcacctcaat ggtaaaccgg tagaaaaaat gatcccggta caacccggcg tttatacagg
     1861 taaatagtaa acatcttcac ctcacaacaa aacgttattg gcgttttgtt gtgacaccga
     1921 ggtcattatg acggatgtta ttcttgatgt ttcgcatatt gcgaagacat ttggtcatgt
     1981 tcaagcatta aaagatatta ccctctcttt acgtaaaggg cgggtgcata ctttattagg
     2041 cgagaatggt gccggtaaat cgacattaat gaaaatactg gccggtgttt atccgccaac
     2101 ccagggaacg ataacactac gtggtgaaac tatcaccatt aataatccgc agcattcacg
     2161 ccagttagga attgcgatta tttttcagga attgagcctg agtaataata tgaccgtggc
     2221 ggaaaatatt tatgccaata atgaaccgcg acgttttggc attattaacg ataaaaaaat
     2281 gctcgcagat tgccagaatc tcctcgcgga actgggcata ccactagacc cactagaaat
     2341 ggtcggcaat atgtcgatgg cacataggca actggtagaa attgctaaag ccttaagcta
     2401 tgcagcggat gtcgtgatta tggatgaacc gacatcttcg ctaagtgata atgaagcgga
     2461 gattctgttt aacattattg aaaaattaaa gcagcgaggc tgtgcggtta tttatatctc
     2521 tcatcgcatg gatgaaatta tgcggatttc agatgatatt tcggtgatcc gcgacggtga
     2581 gtatatcgcc acgcatgaaa agaaaaacag tgatatccag catttgattg cccaaatggt
     2641 tggtcgggaa atgaaaaata tttggccagc acgtcttggg gaaataccgg atgaaaatgt
     2701 tcctgcaaaa ctggaagtaa aaaatctttc ccatccttcg ctttttaaag agatcagttt
     2761 cgctgtgcgt ccgggggagg tcctcggatt ttttggcctg gtgggggccg ggcgttccga
     2821 tgtgatgaag gcgctctttg gcctcgtttc gtatcagggg actgtgttga ttgatggtaa
     2881 ggaagtcagg atcgctaatc ctaaacaggc tattgatcat ggcattgctt ttgtgaccga
     2941 aaaccgcaag gaagaagggt tagtgctgat gcacgacgtc aatatgaaca cgcaccatgt
     3001 ggcgtttcaa tataacgctt cgcggatggg actcatcaat caccgacaag aagaggcgaa
     3061 gacgctgcaa tcaattgcca gaatgaacac taaagtgagt tctgtacatc aggcagtggg
     3121 agcgctgagc ggcggtaatc aacagaaaat cgtgttatcg aagtggctgg agaaaactcc
     3181 gcgtatttta ttgctcgatg agccaacgcg cggagtggat gtcggcgcaa aatttgaaat
     3241 ctataacgtg atacgtcaac tggctgcggc agggacagca attattctgg tgtcctctga
     3301 actgccagaa gtcatggcac ttagcgatcg tctggtggta atgcgtaata aaacgattgc
     3361 cgatatttat tcatgtgaaa atttaacgca gacacaggta atgaccgctg caacaggagt
     3421 acgttaaatg acagagaaaa ctcttgccag tccgaaacct tcggaaattc ccattgcaaa
     3481 taattttaaa aaagtcttca ggcaatatgg cggtatcctc agtggaatgg tggtgttaat
     3541 cattctgttc agttttatta atgacagttt ttttaccgcc aacaacatta ctaatattat
     3601 tcttcaggtt tcgataattg caattaccgc ctatggaatg acttatgtac ttttgcttgg
     3661 cgatattgat ttatcggtcg gttcaaccat tgcattgatt ggcacatttg ctgcgcttgg
     3721 ggcttcatgg ggaattccat ttattttatt ggtcccatta tccattattg cagcactggc
     3781 tctggggatg ataaatggtg gcttgacggc tatagcgggg atcccgtcgt ttattgtcac
     3841 tgttgctact atggggatct tccgtggtat cgcttatata gtgaccgatg gtatgcccat
     3901 catgattaaa gatgatgcct ttctggcact ggggaatgga gaatttctct atatacctat
     3961 tccaatctgg atattaatca tcttattgct gattaaccac ttcattctta ctaaaacaac
     4021 ctttggacga aaaatttata tcactggtgg taataaagaa gcggcaattt attcagggat
     4081 aaatgtcacc cgcttgaaaa ttaaagtctt tatgattaca gccgtgctgg caggtatcag
     4141 cgggatgatc ctcgcctcac gccgctattc cgggcaaccg aattccgcgt tgagctacga
     4201 actggatgct attgctgcgg ctgtactggg aggcaccagc ctgaacggcg gctatggaac
     4261 cgtggtgggg acggtgatcg gcgcattaac tatcggcgtt attaataatg gcatgaactt
     4321 aatgaatgtt ccctattttt accagatggt ggttaaagga ttagtcattc ttgtagccgt
     4381 ttattttgac gtaagaaata aaagaaaacg cagctaatca tttcaaggaa ttaatcatat
     4441 gagtaaggtc ttcacaattg gagaaattct ggttgaaatt atggcgagta aaatcggcca
     4501 gccatttgat caaccaggta tctggaacgg cccttatccc agtggtgcgc ccgctatttt
     4561 tatcgaccag gtgacacgtc ttggcgttcc gtgcggcatc attagctgtg tggggaatga
     4621 tggctttggt gatattaata tacatcgtct ggctgctgac ggtgtggata tcagggggat
     4681 ttcagtctta cctctggaag ctaccgggag tgcctttgtt acttatcata actctggcga
     4741 tcgcgatttt atttttaata ttaagaatgc cgcttgcggt aaattatcgg cgcagcatgt
     4801 tgatgaaaat attctgaagg actgtaccca ttttcatatt atgggctctt ccctgttttc
     4861 gtttcatatg gtagacgcag taaaaaaagc ggtaactatc gttaaagcaa atggtggtgt
     4921 tatttctttc gaccctaata ttcgtaaaga gatgctcgat attccagaaa tgcgtgacgc
     4981 cctccacttt gttcttgagt taacggatat ctatatgccc agcgagggcg aagtgttgct
     5041 cttgtcgcca cattcgacgc cggagcgtgc aatcgcaggt tttcttgaag aaggtgttaa
     5101 agaggtgatt gtgaagcgcg gcaatcaagg tgccagttac tactccgcga atgagcagtt
     5161 tcatgttgag agctatccgg tggaagaagt tgacccgacc ggtgcgggag actgctttgg
     5221 tggcgcgtgg attgcctgcc gccagctagg gtttgacgcc catcgagcgc tgcaatatgc
     5281 caatgcctgc ggtgcgctgg cggtaaccag acgtggccca atggagggaa cgtctcgcct
     5341 tgcggaaatc gaagcgttca tccagcgcca tgacatgtcc attcgggaag cagcacaatg
     5401 aaaaacgaaa tttggtcgtg gattggcgat ctctcgcagg ttgccggaat taaacactat
     5461 gagttacgta gtggcagagc aaagggtaca gaagcgtttg atgtccgtac tggtgcggga
     5521 ctggcattta cggtggtaaa agatcgcgcg ctggatattg catgggccag ctacaaggac
     5581 acggcgcttt cttttatcac ccccaacggc attgtcgccc ccgctttttt tgagtctcag
     5641 ggcaacggat ttttacgtag cttttacgcg gggttactga ctacctgcgg acttagctac
     5701 attggcacgc cttgtgagga tgaaggagaa acgctggggt tacacgggcg tttggctgcg
     5761 actccagcag aagaggtggg gtatcgcaca gaacggaccg acgacggcat tgaatttgtc
     5821 atcaatggca aagtacggga aacgcgactg ttcggcgaaa atctgacgct ggaaaggacc
     5881 attcgctgcc gttatggcga aaatgtgctg cgcattgaag acaaggtgac taatcatggt
     5941 ttcacccgcc agccgctgca aattctttat cactttaatt acggctggcc tttattatcg
     6001 ccacaggcag aaatcttgct gtcggctaaa agcgtaacgc cacgaactcc gcatgcggcg
     6061 gaagggttaa ccagtcatct ggagatttgt accccacagc ccggttttga cgagcaggtt
     6121 tactatctca cgttgaatag cgacagtcag gggatgagta aagttgctct ggtgaacgcc
     6181 gaactggggt ggggaattta cgagaaattc gacaccatgc agctacccaa ctttatccag
     6241 tggaaaaatt taggcgcggg tgagtatgtt atgggactgg aggtcagtaa tagttttcct
     6301 gacggtcgtg ataaagaacg agcgcagggg cgtctgccct ttattgagcc aggggaaaca
     6361 aagaaatact gctttgagct gggaattgtt gatggcgatg ctgaaatgag cgcgttgaaa
     6421 gcggaaattg caggctaccg ttagctaacg gcaataaaaa cgccgggcag cgttaacagg
     6481 cccggcgttg attacgttct ttgctaagcg cgatgaaaac accgcgcgtc acaaattatt
     6541 tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca tgctggaagt
     6601 tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg tcgatgatca
     6661 gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa
     6721 tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg tagatatgag
     6781 actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg cctgggcttg
     6841 gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg ccgcgaacgt
     6901 gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc tgttctttga
     6961 tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata cgggtgttca
     7021 tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct gcaccgcgat
     7081 agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc agttccgggg
     7141 taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc atggagcagt
     7201 cacgttccgc cagatagata gcgttgccct gaccgtcagc cagtacctga atctcgacgt
     7261 ggcgaggatt ttccaggtat ttctccatgt aaaccatatc gttgctgaaa gcagctttcg
     7321 cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc actacgcgca
     7381 taccgcgacc accgccgccg ccggaggctt tgataatcac cggataacca atgcgtttag
     7441 caatggcacg gtttttatcc atatcgtcgc ccaacgggcc gtcagaaccc ggtacgcaag
     7501 ggacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc aggcgaatgg
     7561 tttctgcttt cgggccgatg aagataaagc cggagcgttc aacctgctcg gcaaagttgg
     7621 cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg atttcagcgg
     7681 cgctgatgat tgccgggatg ttcagataac tttttactga cggagcaggg ccaatacaga
     7741 ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgcgctggag tgcacagcga
     7801 cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc tcgccgcggt
     7861 tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac cagcggctcg
     7921 tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc ggatttgtcc
     7981 gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc gcccacgttg
     8041 actttctgac ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg gtagaaagta
     8101 ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg cgcttccatg
     8161 gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat tattggtgca
     8221 gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct aatgcgtact
     8281 gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac cagctcgatc
     8341 agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt agtgtgattg
     8401 tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat gcatagcgat
     8461 aaattccagc aggccgggac gccggtctat tttgccccag agtgtcaatg ttagacttga
     8521 cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat accttcgttg
     8581 cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt
     8641 ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaag ataatcaaca
     8701 gctaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac aattcacgaa
     8761 attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctgt aaagccagcc
     8821 ctgcgaagat gaggggctgc aatgagataa tcttcacaga ccacaaataa tgtagggatg
     8881 ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta cccaattttc
     8941 actgcattgt ctggatagat gttaatgcta aagcacgcaa aattaaccga ttgacaatgc
     9001 tcagcattaa ataggttcga gtgattaatt tgtagtcaaa caaaatcaga ttattccccc
     9061 cattttgcag aaggtaaact gttttgtcag gcggcagtgc ggctatctga tgctttcaga
     9121 atttgccacc tgacaataaa aaagccgatt tggtagcaaa atcggcttcg gtgagtttgc
     9181 agttttcatc acatactccg acaagctttt tcaagcatga cggaggttgc gttaatgcaa
     9241 ggtttctacc cagggataaa agtgaccgtt acggcggatg aatatctgtg ttgtcagagg
     9301 atggtcatcc tcacggaagc attgttttgt tagctgtcgt aatgcactga gatttgctgg
     9361 ctgatcattg tgggtgaaaa acagttttcc tctggaggga acggtcacta acaggtcgcc
     9421 gcattcagga tcgggtgatt gcacccaaag atcgtcaatg agcatcagac tgggctcata
     9481 agtaccccct gcggagataa cggcaacttt tccatcgttt gcgcctgtat caacgatttc
     9541 agccgtaact tccggcaata tttgcttcaa attactgatg gcttgttcgc ggtcaatggt
     9601 cacttcgtag cgcatcagat agcgcatggt ttcgctacca tcttcagcaa acaagataat
     9661 taactcatcg ttaaagtctt cccaggcaaa atccagcgag gcgttgagga cctctggatc
     9721 agactcatac tggctaagct gttggcgaac ggtatccagc caggcttttt ctttaatcac
     9781 cggaacaata cagcgcccgg tcggcagcat ttctgcaagc tgtttcagca tatctgcagc
     9841 cacttcttgc agcgggtctg gtgcggcttg ataacgtttg taggcatcat caagggtgat
     9901 ttccaccgtt tggtgacccg tatcaatgcg catcaatttg tggtcagtcg aaatcatgac
     9961 tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg
    10021 gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta
    10081 ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt
    10141 aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt
    10201 atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg
    10261 tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac
    10321 cgtggttggc agagtaaaac cacccgccag accacaggca gccacgcagc cgccgtaatt
    10381 catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca
    10441 gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa tacgactggc
    10501 acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata
    10561 acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac
    10621 gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat
    10681 ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat
    10741 ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc
    10801 accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct
    10861 ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt
    10921 tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcaac
    10981 cgtgacatcg ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg cgagagtttt
    11041 gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt
    11101 ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat
    11161 aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac
    11221 aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat
    11281 ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag
    11341 tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag
    11401 cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct
    11461 gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg
    11521 ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct
    11581 gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc
    11641 gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac gctggttaca
    11701 acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga
    11761 gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct
    11821 agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct
    11881 gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga
    11941 taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat
    12001 gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca gccaggttga
    12061 agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc
    12121 actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa
    12181 agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa
    12241 aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga
    12301 taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg
    12361 caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg
    12421 gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc
    12481 ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa
    12541 tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga
    12601 gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa
    12661 tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg
    12721 cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga
    12781 ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt
    12841 aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa
    12901 agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg
    12961 aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca
    13021 agtttacgcc accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt
    13081 tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa
    13141 atattcacaa agatactcgg tttaacctgc cgtttaatct gttttcacgt agaataacgc
    13201 gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag
    13261 atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc
    13321 tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag
    13381 atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc
    13441 cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca
    13501 atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca
    13561 tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa
    13621 gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg
    13681 ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc
    13741 tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc
    13801 tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc
    13861 agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg
    13921 ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca
    13981 ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg
    14041 gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg
    14101 gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag
    14161 tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc
    14221 tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg
    14281 taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca
    14341 tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa
    14401 ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca
    14461 aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg
    14521 caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat
    14581 gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt
    14641 ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta
    14701 tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga
    14761 tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg
    14821 tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa
    14881 cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac
    14941 tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag
    15001 cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt
    15061 ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct
    15121 gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga
    15181 tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt
    15241 gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt
    15301 aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca
    15361 acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac
    15421 gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt
    15481 gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc
    15541 gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc
    15601 tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc
    15661 gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg ggtaatctgg
    15721 ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt
    15781 ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc
    15841 gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg
    15901 cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag
    15961 cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg
    16021 gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa
    16081 gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt
    16141 aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt
    16201 ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg
    16261 ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc
    16321 tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg
    16381 gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc
    16441 atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg
    16501 cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa
    16561 cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag
    16621 gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc
    16681 acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac
    16741 tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa
    16801 acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg
    16861 cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg
    16921 caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atccgatatc
    16981 atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc
    17041 tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc
    17101 actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtgtttatg
    17161 ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc
    17221 ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca
    17281 gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg
    17341 accaaagtta tggagcgtaa aaaacgcccg cagacccgtt atcagctgta cggcgaactg
    17401 gcgctggcgc agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt
    17461 gactcacgcc tgacttacga agcattgctg gagtttacct cggagtacat tcccgagatg
    17521 accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa
    17581 aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctgatt
    17641 atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat
    17701 cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc
    17761 cagttacggt tgcgtaatct gggtgggatt atcattattg atttcatcga tatgaataat
    17821 gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg
    17881 aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc
    17941 cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg
    18001 gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat
    18061 gcttacgact ccgaccgttt cctggtctat gcatctccgg cagtagctga agccttgaaa
    18121 ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta
    18181 caaattgaac cgctctataa ccaggagcag tttgatgtcg taatgatgta aacagatgct
    18241 gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt
    18301 gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgctct
    18361 gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct
    18421 caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg
    18481 gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg
    18541 cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat
    18601 gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat
    18661 caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg
    18721 tcaatttgac catttcgatc tccgcgacag tgaagtcagt tttctgacgc catccggtca
    18781 gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc
    18841 ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat
    18901 ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac aggcggatga
    18961 catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc tggaaacggc
    19021 acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt
    19081 ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata cgctgtcggt
    19141 ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga
    19201 tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctggat
    19261 accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc
    19321 cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc
    19381 tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct
    19441 ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc
    19501 ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag
    19561 cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt
    19621 attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga
    19681 caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg
    19741 cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg
    19801 catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa
    19861 agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct
    19921 ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt
    19981 gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa
    20041 ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa
    20101 tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact cgaaagaaaa
    20161 actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga
    20221 gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt
    20281 gaatgctcgc ttacatcttg atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga
    20341 agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt
    20401 gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg
    20461 gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt
    20521 tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc agcgttctgc ctgaagcggt
    20581 gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta
    20641 tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg tgagtagtca
    20701 cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga
    20761 tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc atttcaatag
    20821 ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa
    20881 aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg
    20941 tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag
    21001 tttcccacaa cacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa
    21061 taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg cgcaagggcg
    21121 tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga atatcaaata
    21181 tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt
    21241 cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac gatgcacaga
    21301 gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca tttctggcga
    21361 tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg
    21421 tgaatgggtt aataatccgg agaatgaacg tacctcgctg aaaggaaaac tgcgcgggca
    21481 gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt
    21541 taatgtggat tacgatttac actggcgcaa agcaccctgg caaccagatg aggcgacgtt
    21601 gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca
    21661 tgccgggcaa ttgctgcgct tattgagcgt tgatgccctg atgcgtaagc tgcgttttga
    21721 tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat
    21781 taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc
    21841 catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc
    21901 agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc
    21961 ggcagtattt gcagccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg
    22021 ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg
    22081 taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat
    22141 cgttgccaaa ggccaacgag ccggaacata actgtaggcc ggatagggcg ttcacgccgc
    22201 atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt
    22261 ctgaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc
    22321 gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg
    22381 agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc
    22441 gatcagggcg ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc ttacgctgac
    22501 caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat
    22561 agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg
    22621 gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat
    22681 aaggttgccc gcgaagcgga caagcgcgta caagaagtga ctgccagcct cagcggcgtt
    22741 tatgaattaa ttctggttgc ggccaccgac ggcacgctgg cggcggatgt tcgtccgctg
    22801 gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt
    22861 ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt
    22921 gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct
    22981 gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat
    23041 gaagcggtag gtcacggtct ggaaggcgac ttcaactgcc gcggcacttc agtatttagt
    23101 gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg
    23161 gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg
    23221 ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg
    23281 gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt
    23341 atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt
    23401 gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatattac atccggcaaa
    23461 ttcgttttct ccacttcaga agcgtatctg attgaaaacg gtaaagtaac gaagccggtg
    23521 aaaggcgcga cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc
    23581 aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg
    23641 gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa
    23701 taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc
    23761 tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca
    23821 atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa
    23881 caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt
    23941 cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca
    24001 gttcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg atcccggcac
    24061 ccgccgttag ccagcgcacc agcgtcatcg gatcattagt cacaaatctc ccttgcggga
    24121 tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc
    24181 tgtattcaag ccatgaatgg ctacttaaat cggcgggttt ttccggtatg ccgtattgtg
    24241 tgagatagct tttcgcggcg cacaccacca ttggcatcgc gccaagacgg cgggaaaaca
    24301 ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt
    24361 cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt
    24421 tggctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca atgcgtagcg
    24481 tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa
    24541 gcatacgacg gcagccctgg tagtaaattc taccggcttc ggtcaggcca atgctgcgtg
    24601 tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga
    24661 tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta
    24721 ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga
    24781 tttagatcac ataatataga taacagcata acagttacgc taatatatta aatatcaatc
    24841 tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa
    24901 aataataacg cctgctctct ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc
    24961 gtggtgtttg ggctgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt
    25021 ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg
    25081 gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt
    25141 tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt
    25201 agtcattctg gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg
    25261 gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact
    25321 cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac
    25381 catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcctatta
    25441 tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca
    25501 ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg ttctgcatca
    25561 gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat
    25621 ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac
    25681 tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat
    25741 ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg
    25801 cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gctggggtca ccaacgccag
    25861 cagcacgcgt gacgacaaag ggatggcgac catcgactct aaccttgaat gggtgcgttt
    25921 ggcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc
    25981 gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc
    26041 gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc
    26101 tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg
    26161 ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg
    26221 attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc
    26281 tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc
    26341 atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt
    26401 ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc
    26461 ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag
    26521 tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat
    26581 ttgctctttt ctccgcgatc gatcaaacaa gaagtggatc gagagctgga aagtttgctg
    26641 gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat
    26701 aaagcctggg gcgacctggt gcgacgcacc acggcgctac aaagcatgcg cagcaacctg
    26761 aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta
    26821 tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa
    26881 ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc
    26941 cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct
    27001 gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt
    27061 atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagtc
    27121 aaagtagagt cagccgaacg tcatcatgcg atggttaact tctggcgaac cacactttcc
    27181 tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg
    27241 gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg
    27301 atcgacttta tctacgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg
    27361 attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga
    27421 tttttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc gctggccagc
    27481 accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac
    27541 agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gcttgtgcgg
    27601 gataaatcgc gcgacaggac cggacgtgta ctgcttaatc agtttgtttc tgccgctgtt
    27661 tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag
    27721 cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg
    27781 acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta
    27841 tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat
    27901 gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag
    27961 ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg
    28021 ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc
    28081 ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg
    28141 gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta
    28201 tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact
    28261 atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct
    28321 gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga
    28381 agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa
    28441 ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg
    28501 tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc
    28561 gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat
    28621 acggtcccga ttgcttcacg attttgtgct tgtgcagcat caatggagtc ggcagcgaat
    28681 gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg
    28741 atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat atcacaaaat
    28801 caagtataca ct
//
LOCUS       DAZFDP010000047        28691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFDP010000047 DAZFDP010000000
VERSION     DAZFDP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..344
                     /locus_tag="ACHJ1G_002136"
                     /pseudo
     CDS             204..344
                     /locus_tag="ACHJ1G_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(426..513)
                     /locus_tag="ACHJ1G_002137"
     tRNA            complement(426..513)
                     /locus_tag="ACHJ1G_002137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Ser,seq:tga)
     gene            940..2058
                     /gene="hyaA"
                     /locus_tag="ACHJ1G_002138"
     CDS             940..2058
                     /gene="hyaA"
                     /locus_tag="ACHJ1G_002138"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4763991.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2055..3848
                     /gene="hyaB"
                     /locus_tag="ACHJ1G_002139"
     CDS             2055..3848
                     /gene="hyaB"
                     /locus_tag="ACHJ1G_002139"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4763992.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3867..4574
                     /gene="hyaC"
                     /locus_tag="ACHJ1G_002140"
     CDS             3867..4574
                     /gene="hyaC"
                     /locus_tag="ACHJ1G_002140"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4763993.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4571..5158
                     /gene="hyaD"
                     /locus_tag="ACHJ1G_002141"
     CDS             4571..5158
                     /gene="hyaD"
                     /locus_tag="ACHJ1G_002141"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4763994.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5155..5553
                     /gene="hyaE"
                     /locus_tag="ACHJ1G_002142"
     CDS             5155..5553
                     /gene="hyaE"
                     /locus_tag="ACHJ1G_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4763995.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5550..6407
                     /gene="hyaF"
                     /locus_tag="ACHJ1G_002143"
     CDS             5550..6407
                     /gene="hyaF"
                     /locus_tag="ACHJ1G_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4763996.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6541..8086
                     /gene="appC"
                     /locus_tag="ACHJ1G_002144"
                     /pseudo
     CDS             6541..8086
                     /gene="appC"
                     /locus_tag="ACHJ1G_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            8098..9234
                     /gene="appB"
                     /locus_tag="ACHJ1G_002145"
     CDS             8098..9234
                     /gene="appB"
                     /locus_tag="ACHJ1G_002145"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4763997.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9247..9339
                     /gene="cbdX"
                     /locus_tag="ACHJ1G_002146"
     CDS             9247..9339
                     /gene="cbdX"
                     /locus_tag="ACHJ1G_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4763998.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9419..10717
                     /gene="appA"
                     /locus_tag="ACHJ1G_002147"
     CDS             9419..10717
                     /gene="appA"
                     /locus_tag="ACHJ1G_002147"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4763999.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10832..13012)
                     /gene="etk"
                     /locus_tag="ACHJ1G_002148"
     CDS             complement(10832..13012)
                     /gene="etk"
                     /locus_tag="ACHJ1G_002148"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4764000.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNIYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13032..13478)
                     /gene="etp"
                     /locus_tag="ACHJ1G_002149"
                     /gene_synonym="yccY"
     CDS             complement(13032..13478)
                     /gene="etp"
                     /locus_tag="ACHJ1G_002149"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4764001.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13466..14605)
                     /gene="gfcE"
                     /locus_tag="ACHJ1G_002150"
     CDS             complement(13466..14605)
                     /gene="gfcE"
                     /locus_tag="ACHJ1G_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4764002.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14651..16747)
                     /gene="gfcD"
                     /locus_tag="ACHJ1G_002151"
     CDS             complement(14651..16747)
                     /gene="gfcD"
                     /locus_tag="ACHJ1G_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4764003.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16747..17493)
                     /gene="gfcC"
                     /locus_tag="ACHJ1G_002152"
     CDS             complement(16747..17493)
                     /gene="gfcC"
                     /locus_tag="ACHJ1G_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4764004.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17490..18134)
                     /gene="gfcB"
                     /locus_tag="ACHJ1G_002153"
     CDS             complement(17490..18134)
                     /gene="gfcB"
                     /locus_tag="ACHJ1G_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4764005.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18241..18546)
                     /gene="gfcA"
                     /locus_tag="ACHJ1G_002154"
     CDS             complement(18241..18546)
                     /gene="gfcA"
                     /locus_tag="ACHJ1G_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO4764006.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(18988..19200)
                     /gene="cspH"
                     /locus_tag="ACHJ1G_002155"
     CDS             complement(18988..19200)
                     /gene="cspH"
                     /locus_tag="ACHJ1G_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4764007.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19486..19698
                     /gene="cspG"
                     /locus_tag="ACHJ1G_002156"
     CDS             19486..19698
                     /gene="cspG"
                     /locus_tag="ACHJ1G_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4764008.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19709..19897
                     /gene="ymcF"
                     /locus_tag="ACHJ1G_002157"
     CDS             19709..19897
                     /gene="ymcF"
                     /locus_tag="ACHJ1G_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4764009.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            19872..20102
                     /gene="ymcE"
                     /locus_tag="ACHJ1G_002158"
     CDS             19872..20102
                     /gene="ymcE"
                     /locus_tag="ACHJ1G_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4764010.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20092..20265
                     /gene="gnsA"
                     /locus_tag="ACHJ1G_002159"
     CDS             20092..20265
                     /gene="gnsA"
                     /locus_tag="ACHJ1G_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4764011.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20313..21386)
                     /gene="yccM"
                     /locus_tag="ACHJ1G_002160"
     CDS             complement(20313..21386)
                     /gene="yccM"
                     /locus_tag="ACHJ1G_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4764012.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21458..24202)
                     /gene="torS"
                     /locus_tag="ACHJ1G_002161"
     CDS             complement(21458..24202)
                     /gene="torS"
                     /locus_tag="ACHJ1G_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4764013.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            complement(24297..25370)
                     /locus_tag="ACHJ1G_002162"
     CDS             complement(24297..25370)
                     /locus_tag="ACHJ1G_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764014.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYERILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     HDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDLMAARYRDSRGREWDKIEIDK"
     gene            complement(25348..25566)
                     /locus_tag="ACHJ1G_002163"
     CDS             complement(25348..25566)
                     /locus_tag="ACHJ1G_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4764015.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(25673..26017)
                     /locus_tag="ACHJ1G_002164"
     CDS             complement(25673..26017)
                     /locus_tag="ACHJ1G_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764016.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(26046..26213)
                     /locus_tag="ACHJ1G_002165"
     CDS             complement(26046..26213)
                     /locus_tag="ACHJ1G_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764017.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(26286..26570)
                     /locus_tag="ACHJ1G_002166"
     CDS             complement(26286..26570)
                     /locus_tag="ACHJ1G_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4764018.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(26563..26706)
                     /locus_tag="ACHJ1G_002167"
     CDS             complement(26563..26706)
                     /locus_tag="ACHJ1G_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764019.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(26716..26865)
                     /locus_tag="ACHJ1G_002168"
                     /pseudo
     CDS             complement(26716..26865)
                     /locus_tag="ACHJ1G_002168"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            complement(27342..27479)
                     /locus_tag="ACHJ1G_002169"
                     /pseudo
     CDS             complement(27342..27479)
                     /locus_tag="ACHJ1G_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27481..28038)
                     /locus_tag="ACHJ1G_002170"
     CDS             complement(27481..28038)
                     /locus_tag="ACHJ1G_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4764020.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(28035..28592)
                     /locus_tag="ACHJ1G_002171"
     CDS             complement(28035..28592)
                     /locus_tag="ACHJ1G_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764021.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(28589..>28691)
                     /locus_tag="ACHJ1G_002172"
     CDS             complement(28589..>28691)
                     /locus_tag="ACHJ1G_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO4764022.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
BASE COUNT         6742 a         7343 c         7240 g         7366 t
ORIGIN      
        1 taggattgct catttttata ccatttgttg ttttttatag cgatttgcta aaagggagaa
       61 gaaaaaacgt tctccaaaat tagattgcaa ctgtttgatt ttatggatag aggcatgctg
      121 tttactagac agtaaaaagc atgccaaatt tgctattaac cttttgaata taaagcacta
      181 attatctatt tctgttgcct tcttcagtag atctggagat cgcaacggag gaagaaaact
      241 cgttgctgga agcctggaag aagtatcggg tgttgctgaa ccgtgttgat acatcaactg
      301 cacctgatat tgagtggccg gaagaaccaa gatcatccga ataaaaatat accaattggt
      361 ataccaagtt tataccaaga gccagaaaag caaaggggct accatttggt aaccccttga
      421 ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc cggttttcaa
      481 gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat aaacatcccg
      541 atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa agtgcattga
      601 tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct cttttactcg
      661 tttagcaacc ggctaaacat ccccaccgcc cggccaaaag aaaaataggt ccatttttat
      721 cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca ctacgcttta
      781 ttaacaatac tttctggcga cgtgtgccag tgcagaagga tgagctttcg ttttcagcat
      841 ctcacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc ataagcgccc
      901 ggtgtcctgc cggtgtcgca aggagaagag acgtgcgata tgaataacga ggaaacattt
      961 taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata ttgtagtctg
     1021 gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg ggcgctggag
     1081 aacaaacccc gcattccggt ggtatggatc cacggtctgg aatgcacctg ctgtaccgaa
     1141 tcttttatcc gctccgctca cccactggcg aaggacgtca tcctttccct gatttccctc
     1201 gattatgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt cttcgaagac
     1261 atcatcacgc aatacaatgg caaatatatc ctcgcagtag aaggtaatcc gccgctgggc
     1321 gagcagggga tgttctgtat cagcagcggt cgaccgttta ttgagaaact caaacgtgcc
     1381 gctgccggag ccagcgcgat tatcgcctgg ggaacctgcg cgtcctgggg ctgcgtgcag
     1441 gccgcgcggc ccaatccgac gcaggcaacg cctatcgaca aagtcatcac cgacaaaccc
     1501 attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat cattacttac
     1561 atggtgacct ttgatcgctt gccagatgtc gacagaatgg gccgtccgct gatgttctat
     1621 ggtcagcgaa tccacgataa atgctatcgc cgcgcccact tcgacgccgg agagttcgtc
     1681 cagagttggg atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat gggctgcaaa
     1741 gggcctacca cctataacgc ctgttcttcc acacgctgga atgatggcgt ttctttccca
     1801 atccagtctg gtcacggctg cctgggctgt gcggaaaatg gtttctggga tcgcggttcg
     1861 ttctacagcc gcgtggtcga tattccgcaa atgggtactc attccaccgc cgataccgtc
     1921 ggtttaaccg cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt cgccagcgcc
     1981 gttgaccagc gcagacgtca taaccagcaa cctacagaaa ccgaacatca gccaggcaat
     2041 gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc aataatgccg
     2101 gacgccgcct ggtggtcgac ccgattacgc gcatcgaagg ccacatgcgc tgcgaagtga
     2161 atattaacga tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg tttcgcgggc
     2221 tggagatcat cctacaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt gaacgtatct
     2281 gcggcgtctg tactggcgta cacgccctgg cttcggttta cgccatcgaa gatgctatcg
     2341 gtattaaagt gccggacaac gcaaatatca tccgcaacat tatgctggca acgctctggt
     2401 gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc gatgtgttag
     2461 atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagtctc tcctcttggc
     2521 cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg cctgaaaaaa tttgttgaag
     2581 gcgggcagtt ggggatcttc cgcaatggct actgggggca cccgcagtac aaactgccgc
     2641 cagaagctaa cctgatgggc tttgcccact atctcgaagc tctcgatttc cagcgtgaaa
     2701 ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca tccaaactgg attgtcggcg
     2761 ggatgccttg cgccatcaac attgacgaaa gcggcgcggt cggggcagtc aatatggaac
     2821 gcctgaacct ggtgcagtca attatcaccc gcacggcgga cttcattaac aacgtgatga
     2881 tccccgacgc cttagccatc ggtcagttca acaaaccgtg gagcgaaatc ggcactggtc
     2941 tttctgataa atgcgttctc agctacggcg cattcccgga tattgccaac gactttggcg
     3001 agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc
     3061 cagtggattt ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc tggtatcgat
     3121 atcccaacga tcaggtcggg cgtcatccgt tcgatggcat caccgacccg tggtacaacc
     3181 ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag gaacgctact
     3241 cgtggatcaa agcgccacgc tggcgcggta acgcgatgga agtggggccg ctggcgcgca
     3301 cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt
     3361 cggcgttgaa cctgccgctt tccggtatcc agtcaacgtt aggccgcatt ttgtgccgcg
     3421 cgcacgaagc gcagtgggcc gcaggtaagt tgcagtattt cttcgacaag ctgatgacca
     3481 acctgaaaaa cggcaatctc gccactgctt ccacggaaaa atgggaacct gcaacctggc
     3541 cgacagagtg ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta ggccactggg
     3601 ccgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc acctggaacg
     3661 ccagcccgcg cgatcccaaa gggcagattg gcgcttatga agcggcgctg atgaacacca
     3721 aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc tttgacccgt
     3781 gcctcgcctg ttcaacacac gtgctgggcg acgacggtag cgagctgatc tctgtgcagg
     3841 tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt gtcagccact
     3901 atgtctttga agcgccagtc cgcatctggc actggttgac ggtgttatgc atggcggtgt
     3961 tgatggtcac cgggtacttt atcggcaagc cgctaccttc cgtcagcggc gaggcgacgt
     4021 atctgttcta tatgggctac atcaggttaa ttcacttcag cgccgggatg gtttttaccg
     4081 tggttttgct gatgcggatc tactgggctt ttgttggcaa tcgatactcc cgcgagctgt
     4141 ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa atccgctggt
     4201 atctgtttct ggcaaaacgt ccgagtgccg atataggcca taatcccatc gcccaggcgg
     4261 cgatgttcgg ctatttcctg atgtcggtct ttatgatcat cactggtttt gcgctgtata
     4321 gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa tttttctact
     4381 ggatgggtgg caactcaatg gacattcaca gctggcatcg gctggggatg tggctgattg
     4441 gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga agacatcatg tccgacgaca
     4501 cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa ataagtaaca
     4561 aggagcgttc atgagcgagc aacgcgtggt ggtcatgggg ctgggcaacc tgctgtgggc
     4621 cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc actggcccga
     4681 gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt atgtcgaaag
     4741 cgccagccat ctgttgattc tcgatgccat tgactacggg ctggaacctg gaacgctgcg
     4801 aacctatgcc ggagaacgca ttccggctta tctcagcgcg aagaaaatga gcctgcatca
     4861 gaacagtttc tccgaagtgt tggcgctggc ggatatccgc ggacatctgc cagcacatat
     4921 tgccctcgtc ggtctgcaac ccgcaatgct cgacgactac ggcggtagcc tgagcgaact
     4981 ggcacgggag caactgcccg ctgcggaaca ggcggcgctg gcgcagctgg ctgcgtgggg
     5041 aattgtgccg caaccggcta atgaatcgcg ctgtctcaat tatgactgtc tgtcgatgga
     5101 aaattacgaa ggcgttcgct tgcgccagta ccggatgaca caggaggagc agggatgagc
     5161 aacgacacgc catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg gacgccagtc
     5221 agtgaatccc gtcttgacga ctggcttacg caagcgccag acggcgtgct gttattaagc
     5281 agtgacccga aacgcacgcc agaggtcagc gataatccgg taatgattgg cgaattactg
     5341 cgcgagtttc ccgactatac atggcaggtg gcgattgctg accttgagca gagcgaagcc
     5401 atcggcgatc gctttggcgt ctttcgcttt cctgccactt tagtgtttac cggcggaaac
     5461 tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct gatgcgcggg
     5521 cttgtcgaac cgcagcagga gcgtgcctca tgagcgaaac atttttccat ctgctggggc
     5581 caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc acctgtcagg
     5641 tgaatgatga accgagtatg gcggccctgg agcaatgtgc tcacagcccg caggtgattg
     5701 cgctgttaaa cgagttacaa catcaactaa gcgaacgcca accgccgttg ggcgaggtgc
     5761 tggcagtcga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat acgcttctcg
     5821 gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtgaaagt gaaatacagg
     5881 aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag ttgctggagg
     5941 acaaactgga ggctggctgc gcgccgctgg cgttgtggca ggcggcaacg caaaatctct
     6001 tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc ctaccgttgg
     6061 cgcatgagtt actggcacat gtacgtaacc ccgacgcgca gccgcacagc attaatctga
     6121 cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt gggccgggaa
     6181 atattcagat tcgtaccatt ggctatggcg agagctatat caacgccacg gggttacgcc
     6241 atgtctggca tttacgctgt acggacacct taaaaggccc gttactggaa agttatgaaa
     6301 tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac tctgcgcagc
     6361 ggcttagcga ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa gacggtaagt
     6421 atcgctttca gtcttatgaa tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg
     6481 ataaagaggg agatctacca ttatcgggtt atttttctct cttcgcctac aggagtgcgc
     6541 atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgacctgcgc tgtatcactt
     6601 tttatttgta ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta
     6661 cgtggtcacc ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg
     6721 tatcaatttt gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa
     6781 ctggtcattc tattccaact atgtgggcga tatttttggc gcaccactgg cgatggaagc
     6841 attaatggcc ttcttcctcg aatccacctt tgtcgggctg ttcttcttcg gctggcaacg
     6901 gctgaataaa taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc
     6961 tgcgttgtgg atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga
     7021 tatcgacacc ctgcgtatgg agatgaccag cttcagcgag ctggtcttta acccggtcag
     7081 ccaggtgaaa tttgtgcaca ccgtaatggc gggctacgtg accggggcca tgtttattat
     7141 ggcgatcagc gcctggtatt tactgcgcgg acgggagcgc gatgtcgcat tacgttcgtt
     7201 tgccatcggt tccgtcttcg gtactctggc gattatcggt accctgcaac tcggagacag
     7261 ttctgcgtat gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg
     7321 gcaaacggaa cctgcacctg caccgttcca tgtggttgcc tggccggaac aggatcaaga
     7381 gcgtaacgcc tttgccatca aaattcccgc gctgctaggg atcctcgcca ctcactcatt
     7441 agataaaccc gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg
     7501 cggacgtatg gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt
     7561 gttgcaggca ttccgggaac tggaaggtga cctgggctac ggcatgttgc tctcccgcta
     7621 tgcgccggat atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat
     7681 acctcaggtt gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgct
     7741 gctactggtg atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg
     7801 ctgggtgctg aaaatggcgc tctggagttt gccgttgccg tggattgcga ttgaagccgg
     7861 gtggtttatg accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata
     7921 ctccgcgcac tccgctttaa ccacaggaca actggctttc tcactgatca tgatcgtagg
     7981 gctttacacc ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg
     8041 gccgagcgcg atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg
     8101 tttgattatg aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc
     8161 tttatgatct ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt
     8221 aatgatgatg aacgccggat agtgataaac agcgttggtg cacactggga aggcaaccag
     8281 gtctggttga tcctcgctgg tggggcatta tttgccgcct ggcccagagt gtatgcagcg
     8341 gcgttttccg gcttttatgt ggcgatgatc ctggtgctgt gctcactgtt cttccgcccg
     8401 ctggcctttg attatcgcgg aaaaatcgcc gatgcacgct ggcgtaaaat gtgggacgcc
     8461 ggtctggtca tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg
     8521 ttgctcggcg tgccgtttgc cttcacaccg caattacgcg tggagtatct cggcagcttc
     8581 tggcaactgc tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtgatt
     8641 ttgcaaggtg gcgtctggtt acaactgaaa actgttggtg tgattcatct gcgttcacag
     8701 ctggccacca aacgcgctgc actgttggtg atgctgtgct ttttgctggc gggttactgg
     8761 ctgtgggtcg gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat
     8821 ccgttaatga aactggtggc agtgctacct agtgcctgga tgaataattt cgtcgaatcg
     8881 cccgttttgt ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg
     8941 atttatcgtg gtcgcccggg ttggggattt ttaatggcgt cattgatgca atttggcgtg
     9001 attttcacgg caggcatcac gctgttcccc tttgtcatgc cgtcaagcgt gagtccaatc
     9061 tccagcctga cgttgtggga cagcacttcc agtcagctga cgctgagcat tatgttggta
     9121 atcgtgctga tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg
     9181 tggggacgca tgacaacaga aactctccgc cgtaacgaaa acgagttgta ctaaggagca
     9241 ggaacaatgt ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc
     9301 cttgtgttgg tatggctgga cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc
     9361 gcattagcat cgcatcaggc aatcaataat gtcagatatg aaaagcggaa acatatcgat
     9421 gaaagcgatc ttaatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt
     9481 cgctcagagt gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt
     9541 gcgtgctcca accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac
     9601 ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg cctatctcgg
     9661 acattaccaa cgccagcgtc tggtagccga cggattgctg gcgaaaaagg gctgcccgca
     9721 gtctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc
     9781 cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc
     9841 cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg ataatgcgaa
     9901 cgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg
     9961 gcaaacggcg tttcgcgaac tggaacgggt gcttaatttt ccgcaatcaa acttgtgcct
    10021 taaacgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa
    10081 ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga
    10141 gatatttctc ctgcaacaag cacagggaat gccggagccg gggtggggaa ggatcaccga
    10201 ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt tgctacaacg
    10261 cacgccagag gttgcccgca gccgcgccac cccgttatta gatttgatca agacagcgtt
    10321 gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt
    10381 tatcgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac
    10441 gcttcccggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg
    10501 tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca
    10561 gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct
    10621 ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat
    10681 cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt
    10741 acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag accggaaaag
    10801 gcgttcacgc cgcatccggc cactttcagt tttactcttt ctcggagtaa ctataaccgt
    10861 aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggatag
    10921 cgcctttaat attgacgcct gcctgttcca gacgctgcat tgacaaactc acctctttgg
    10981 cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc aacagaacgc cccacgaccg
    11041 cggcatcact caccgccagc atcggcggcg tatcgacaat caccagatcg taatggtcgt
    11101 tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac gggttaggtg
    11161 gcacctgacc gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg atcactttgt
    11221 tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc acggtaaaca
    11281 ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt tgatcggact
    11341 gggcgatcac cgctgccaga gttgaactga caaacgtttt accactgtct ggcgtcgcac
    11401 cggtgatcat cagaatgtta ttctccgtct ccatcatagc gaaatgcaga ctggttcgta
    11461 gcgcacgtac ggcttccaca gcagaatccg ccgggttatc caccgccagg aaggggatat
    11521 ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga cgggtgcgtt
    11581 tatccagcca ctcggacatt gggatagtgg cataaacgct gatgccgtgc tcttccagtt
    11641 gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca cccacagaaa
    11701 taaacaggcc aagaataaaa ccaagcacca cattcaacgc ttttttcggt ttcactggct
    11761 gcggctgagt gactgccggg tcgataatcc gcacgttacc aatggcactg gatttcgaaa
    11821 tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc gcttctacgt
    11881 cacgacttaa acgcaacact tcctgttggg tggaaggcat tgccgatacc cgcttattca
    11941 ggcgtttgcg ttcttgctcc agcgtctggc gtttttccag cagcgcacga taagttgggt
    12001 gatctttctt atacagctgg gagatctctg cctcgcggaa ggtcagctca ttgagttgat
    12061 tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc caggttaagg tcaaccgaat
    12121 cgcgctgctg gcgataaatg ttgagttttt cttccgcctg gtccagctcg ctgcgcactt
    12181 caggtaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga
    12241 tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc aactggggat
    12301 catcaccagt catggtaagt tccagcatcc cgctttcttt actgcgttcg ctaacggtaa
    12361 aggtttcctg caatgcgtta atcgcttcca gttcggtacg ctggctcagg acaaactgtg
    12421 ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccatctttt tccagacgct
    12481 ggccgaccat accattgacg gtgaactctt caccttccag tgtatagtgg ccgttttccc
    12541 caaccgtgag tgtcagttgc tgatcctgac cattcagttg tggaatatgc atccagctga
    12601 tcgccagctc acctggtttt tctttggtta atctcgccca gccgcgaccc acaatcggaa
    12661 aatacttctg ctcaactatg tcgcgcagat tcagttcagc aatggtttta ccgagaatca
    12721 tgcgcgattg cagcagttgg atctccggtg cagactcggg cgatgagtta gggatcatat
    12781 cgctcaggcc gctgagaatg gcgttgccct gtttttgctc aacctggacc agagtatctg
    12841 cctgataaat tggtgtgctt aacagcgagt aagcgacagc gatcagcgtg aataacgcgg
    12901 tcacgctgat aataaactta cggtgatccc ataactcgcc gaccagacga agcagatcga
    12961 tctcattttc ctgagtgctg cctggtggcg tattcatatt tttagttgtc atcgtatttt
    13021 ataaactcaa attaccggct gaggcgcttc gcccattcct gactggcgcg ctccaacata
    13081 ccgtagacat gttcaaatgc gtcctgactt ttacgatagg gatccgggat ctctttctgt
    13141 tccagccatt gcccaaacag cattgtcttc ccgcggacct cgggagcaat tgcggtaacc
    13201 tgagcaatat gttccgactc cattgccaga atcagatcgt aatttcgtgc catctcagca
    13261 gtgagcttac gtccggcatg tccttcaaga gatacgccat gattagcggc aacatctgcc
    13321 gctgtcgcat ctgcagggtg ttttaccaga ccatgaacac ctgccgattt gacttttacg
    13381 cccggcagac gtttacgcag caagcgctcg ccaatgggcg aacggcaaat gttgccggta
    13441 caaaccacca ggattgagtt aaattttagt tgggccatgt ttgaatccac cgtgaagtct
    13501 ctgtcaggtc atgcaccccg gaaatcgtcg gtaccagcag agaaatcaca cggttccaac
    13561 gagccagcgg cgcggtagtg acataaacaa tgtcgtaagg ttcaagctgg aattccgttc
    13621 cgagtatcat tgccgatgcg tccttcgcat tcagctgata aatattggcg attttgccat
    13681 tctgcttgtt ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg acatcctgat
    13741 tcataccctc agcgttaccc agcgcttccg ccagggtcat accgctgcgg tccattttca
    13801 gcgtgctctg tttacccact tcacccatca cgaagacttt cagatcatcg ttgcgcggaa
    13861 taaataaaat atcgcccggg tgcagcagtt tgttctggcg caagtcgcca cgctgcatca
    13921 gcgcatagag gttcactttg gtctttacgc cattctgggt gagcaccacg ttgcgccagt
    13981 ctgcatctgc ggtcagaccg cccgcggcgt taatcgcatc catgatggtc agaggaatat
    14041 tggtaatcgg ctgctgaccc gatttgctga cttcgccggt gacgtacgct ttttgcgagc
    14101 ggaacgccgc gacgctgaca tcgacttgcg ggctttcaat gactgaatcc agacgcgctg
    14161 taatctcgtt gcgtacctgg gtaagtgttt tcccggccac tttcaggcga ccaatatagg
    14221 ggtagaaaat cgcaccatcg gcgttaaccc agttgccggt atcactggcg ctacggtatt
    14281 gccctgcggg ggtggttagt tccgggtgat cccagaccgt aaccatcaac acatccccaa
    14341 tgccgatacg atattcatag tttgcgattt gctgctccag ctccgggtta gcctgcgaca
    14401 taatcggttt ggcgcgcaac tgatcaatca atcctggcgt gacgggatag atattgacca
    14461 ttttgtccag gtcatactga ttgtcaggta gttcaatgac atccttgtta ctggtcgata
    14521 aattctgacc aggaacaagg gtacatgctg tcaaagacag aacagccaat gtcagtaccg
    14581 aaaacttaaa aatgttcttt ttcattggtt tatatttatc agtcaatgta atgttatgaa
    14641 ttacgtcagg ttattgtcca accggtactt ctcgctctgc cgtcattgga tagagctggt
    14701 atttgcgtga cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc
    14761 ggttgcggtt aggtccaatg gtcatcaagt cgaatggaat cgagatataa aaacctttac
    14821 taaagccgcc ttcgccgtag tcatctttag atacgttact gattgccgcc cataccccta
    14881 ccgccacgcc gctgtcaaag cgtttggcga cgtcgatcgt tgcgccttta tcttttgcca
    14941 gatattgccc aacgctaagt ttcatcagta cgccattgag cgtcggcggg ttccagtaag
    15001 ccgtcacgaa acctgttggc gtggaataat cggtgaaacg catcatgttg tcccagtcac
    15061 gttgcttcac gtagttaacg tccacaccca gcgcccagct ggcatctagc gggcgataaa
    15121 gcagctcgga accgacaccg gcgtacatcg tttccagata accgccatac acctggccat
    15181 agaaaccatt gcctaagtcg gcaaagtagt tcgcctgcaa gttgttgaga taaacgtcat
    15241 tgcgaacgta atcacggata tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca
    15301 gcgaagactt aaacttgtcg tagttgttgt aaatattggt gaaaataccg ccatcaagca
    15361 gcaggtggtc ggtaaaccag tagcgggcac tggacatcag ccccagctgg aacatataga
    15421 aatcttccgg gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat
    15481 cttcacgaat acggtagccc cgaccaaagg cagaaagatc ttctgcttca acacgttgtt
    15541 gttgaagttg ctctgattga ccaggcgctg taccagccag ctgtttgcgc aggctggcta
    15601 cgtcggtttc ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct
    15661 caacgccttg cggcaggtta ttcaccagaa tccggttggc acgatcgacc gcttcgcgag
    15721 aatcacggta tttgtattgt tgaccagaca tatacagcgt cttatcgcgc agctgaattt
    15781 ccggtgcttc aaaacctgcg ttgtacttca gcgcggtaag ttggttcgct actgtggtgt
    15841 actgcaatcc ttcagattca ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg
    15901 cagggcgcag atcgttgaaa ttggtccgta acgtgaagcc aaacatcaac gtgttaccgc
    15961 gttcataact caggttgaga tctgcccagc tggcagcgcg ataaactgcg ccgacgttga
    16021 aatggcttgc ctgaggcagt ttgccagcga aatcattctg gtaattgttg ccatcgtatt
    16081 cgagtttcag acgcagggga ttccacggcg tttgatactc aatgccgcca aagatggaag
    16141 ccgggccacg aaagatatcg ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat
    16201 gacaatattt atcgctcacc cggcaaaacg ggttggtaat attgcccgca ttaccggcgt
    16261 agccccatgc catcccgagg gtgaaatcaa atggccccgc ttgcttgctg gccaccagat
    16321 actcaccgtc aaacagaccc gtaccagcaa tatcacgttt accaaacgcc acttgcggta
    16381 gccagtaacc ttcttcccac aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact
    16441 gatcgccgct gaaatcttcc cactggctat atttgcgtgt gcgcacatcc gtataacgaa
    16501 tggtgccttc cagccatggg aacagcgcca cggaggtgga gtagaaccgg tattgatcgt
    16561 tatcccggta gttgacgctg aattcacctt ccggtgcgat gcgcgcattt ggcatctgca
    16621 acaatcctgt gccgccaaag tctgattgcg acgaacccag cggatccggg taggttaata
    16681 cttcagcatg acaggcactg gagacggcaa tggccaggca gcttaaaaga taagaattct
    16741 tcttcatcag tcaggaacac gttgcgttaa aacagacaca atctggtcat ttaaatcagc
    16801 atatttttcg ggcagtacat gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg
    16861 ttccacatgt cttttattcc acagggcaac aggggcgacg acagtctcac cctcaggcgt
    16921 aatcaccata acgttattct tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc
    16981 ggtaacgcta cggcctgcct gccagggcaa ttgcccggcc ccagaaaccg cacccagcag
    17041 agtaatagtc accggacgct gtacggtata gagcgtatag tccccgacca acgggggatt
    17101 gctgttctca tccactcgta caaaatcagg gtccagtttg accggtaagc gaccagtgat
    17161 attgagattg agtaactgct ggcgcacgga tttaatcgtt gccgccacat cgtcgtcagc
    17221 ttctgcttcc caggaagcaa gctgtgccat gacatgttgg tagtctttaa gcgctttagc
    17281 tttcgccgca gagtcggtca gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg
    17341 ttgtgtcacc aactggacaa cattttccat ggggccaacg gaaagtgtct gttgttcgcc
    17401 aggcagataa atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac
    17461 gctggcaatg aaatacgact gtaatttatt catggtgcgg gtttaagaaa cgtcatttct
    17521 acgggaataa cccctgcgcc taacatttgc cgactttgac gaacctggcc agtagccgaa
    17581 tcgacccaga atgtgttttg ccactgtttt tctggccgcg tggaagtcac ctcttcctgc
    17641 cagacattac acagcacatt gcgacccgca atattcagcg tctctaatcc agcaaaggaa
    17701 aaagtagaac tcagggtcgc agagcgaaaa tggttgtctt cactccagag aatatcgcgc
    17761 gtccagcggc tgccatcttt tattgccagc gcgttacgca gagggtcctg cccggaattt
    17821 gtcacttcca gcagattatt attaagtttg acggttttta aaagacgtcc attgtgggta
    17881 accagcatgg cgttatcctg ggataaccat ttgctttgtt cttgttctat atatcccagt
    17941 accacaaaga ttcgttgccc ttcattaagg cgtaaataca tcgtggaata aggcaacgcc
    18001 tggatctgct gatccgctac ggtgatatcc tgattatcga aaaggctggc gcgaaatgta
    18061 tcgaccatac tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa aatcgataaa
    18121 ataagagggc gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa tctctaaagc
    18181 ccggtacgaa tatcgcaacc gggtatcatt taactttaat ttcgacgcgt taaagcagta
    18241 ttagcgtgta gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc cactgtcatt
    18301 tgctgcaaca actgccaacg tcgccacacc aacagccgtt gccacaccga caactgtcgc
    18361 cgtgccgaca gcgccagcag ccacgccacc agtggtagta gcagtcgtgg ttgcgccggt
    18421 agtggtcgtt gtggtgccag tagtacccgt cgttgcctcg gtcccgtttg ttgcttcagg
    18481 ggcagcaaac gcagctggcg tcgtcaacat gaaggccata aggattgcag aaagcttgtg
    18541 tttcataact tttcctttat tcatcgcatg gacaatacgt acgcttgaga tcaggattgt
    18601 ctactttgag tataacatcc ttgtgaaatc tccctttcct cataccgctt gcactatata
    18661 ctgcgggtag gaaaggcgcg cagagggaaa tataagattg ttaactatcc ccctctggaa
    18721 atacgacttg tgagcataag ataaatctta gcaaaataca aattaaagat aaggaaattg
    18781 ttacgaaagc tattaattat tgttggtaat atagtttcaa gtggtactat agctggctgt
    18841 taaatggcat ttataactat taggtgcaga gatattcgct taaatggatt agtttctttg
    18901 tggaaaagta actggtgtta taatgtatga tgttgactat tgcactattc caataaaacc
    18961 agtcagcttt aaacaagcag cgtcatatta agagagataa acatttgccg ctgttggtcc
    19021 tcgcaggcca tttacgcggc aaaattccac acgtaatcct ggtataagca cttctgcgtc
    19081 gcggggagtg aatgcggaaa tatggacctg aacttcttta cgaccgtcgg aggggataat
    19141 gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa
    19201 acaaattcct tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc
    19261 gatatttaag accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt
    19321 aatagctgcg tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc
    19381 attatcaggc attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta
    19441 ttaaagccct gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg
    19501 actggtttag taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat
    19561 ggcagcaaag acgttttcgt ccatttcact gccatccaga gcaatgaatt ccgcacgctg
    19621 aacgaaaatc agaaagttga attttctatt gagcaggggc aacgtggccc cgcggcagcg
    19681 aacgttgtta cgctctaagg ttgccgttat tactcaacat ctccatttcc gctgtccatg
    19741 ttgtcatggt tcacagtacc gtacatcggc attcgatgtg acggagcgaa accctttggg
    19801 cgctaagtgt attttttgta aatcgacgat gatcaccttt gataacgtcg cgctgcaaat
    19861 acgcacggac catgcgccgc tggatttcac aaaataatat caggctccct cgtggagcct
    19921 tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga
    19981 ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa
    20041 ccaacacctt actggcgtgt tgctgtcttt caagaccaga agaggttaac agtgaatatt
    20101 gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc
    20161 gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa
    20221 atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac
    20281 ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg cgcttgtgga
    20341 attaagcgtc ggtacaattc atccggtacc gggctttgcc atactcccgc atacattgcg
    20401 taaccaatca ccgcaaacat aatccccaga accagtagcg tcattaacca gccagacaac
    20461 gcaaaggctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc
    20521 ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat
    20581 ttatcgaccg ggatccgtga tgggcaattt tttgcgcatt tgccacagtc gatacaactt
    20641 tcggcattgc gacgaatctt aaacggcgac aatagcgaaa ccacgcccat cagcgcgcca
    20701 tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg
    20761 gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat tttaacgtcc
    20821 atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg catcaataat
    20881 gcgatataga gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg aatatccagc
    20941 cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagatcgcc gattaattca
    21001 gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa tagactgatg
    21061 acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctgacctgtt
    21121 gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
    21181 ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca ataccagaac
    21241 gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc
    21301 gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
    21361 cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc ataccaaatg
    21421 ccactattct agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct
    21481 tattcaacca tgcttccaga tcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg
    21541 cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaaac
    21601 tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta tcctgactgg
    21661 cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc
    21721 attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca
    21781 gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt
    21841 cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc tggcgcagcg
    21901 tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca
    21961 gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc agtgcggcag
    22021 caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca acagcaacaa
    22081 cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc gggttatctt
    22141 caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt tttggcacgg
    22201 gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc
    22261 tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata gtcagcccca
    22321 gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt tctgccagtt
    22381 tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc tcgccatcag
    22441 tgcgactacg caggataatg tacccttcgt cagtaaaacg cagggcgttg ctcaacaggt
    22501 tggttataac ctgacgaata cgtcgtggat cgcccattaa cgcgcacggc atatcatcgg
    22561 caattgccgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg
    22621 tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca ttcttgccac
    22681 ctgcttcgat agcggaataa tcgagaatat cgttgaggat ggtcagcaac gattcaccag
    22741 agtcagtaat tgcccgcaaa tcatcacgct gggcgttaag tgcggggtta tctgccagca
    22801 gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca
    22861 gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt cggtgttcta
    22921 tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc tgttcgcggt
    22981 ggcgattcag tgcatgaaca ttgctgcgaa acgcatccat cagccgcccg atggtatcca
    23041 gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc agcagccgtt
    23101 gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc acgcgccaga
    23161 ggatcagaat cagtgcgcaa agagaaacta ttcccagcaa taacaggctg tattgcccgc
    23221 gtgcactcgc tttttccaga tgcgccagtc cgtgctgatt acgcagctca atggtatcga
    23281 ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt tgggcgagag
    23341 tttgcaggtg agtgctgatt tcactgtcct gctgatacag cgccagtaga tcgctatatt
    23401 ggctaacggt agttaacgtt gttgcgacct gcgcacgaac tcccggatct tcaatgcgta
    23461 tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc gttggtgcat
    23521 ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc agagcgctaa
    23581 ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg
    23641 cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg
    23701 ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg
    23761 cggcagcgac tatctgctga ctgagttgcc gttgttgctg gcgtagttgc agacgccgcc
    23821 ccaccagttc cccttgctga cgtaatgaac gggagatctc ctgctcctgt tgttcaatag
    23881 cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt
    23941 gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt
    24001 tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg
    24061 taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc
    24121 atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc
    24181 ttcgggtcag ggttaaattc acggtcggtg cacttaaggt gaataagttg tatatttaaa
    24241 aactctttta ttatcaatga attaatgtaa gtgggtcatt gttggtcaaa ataaaatcat
    24301 ttgtcgattt caattttgtc ccattcccgt ccacggctgt ccctataccg cgccgccatt
    24361 aaatctgatt tatgcccgag aagacgttga gcaaacttat cgccaatctg gttccggtat
    24421 agcctcgctg acaggctacg cagttcatgg aatgttggcg ggtttccatc aaatgagagt
    24481 ccagatgcat ttctcgcctt tgtaaaatac tttgatactg ttttcgggga aagcggatcg
    24541 tgatgctttg atgcgattat agtttcactg ctgctggcct ccctgcattt ctgtagtgta
    24601 tcagccaatg agatattgag cgcgtcaatc gttagcgtta gcggaatggc gagtttagcc
    24661 cctgttttac tctgttcaat gtgaagatgg ttgtcgttta tgtctgacca tttcattctg
    24721 cacaaatcgc cgactctctg ccctgtaacg acggccaaat ccatcgccag ccttagccag
    24781 atagggagag gttcggctgc atggtaaatc tcgacatact cattagctgt cagccttgag
    24841 cgccttactt ctgactttgc tgtacgggtt gctgttaccg gattcgttgc aacatgcccc
    24901 tcggctattg cttcacgaaa aacgtcaaca agggttgacc tgattaattt tgcggaagct
    24961 gctttacctt ctgccacgta ggtgtttagc attgctgcca cttctttcgt tgatatgtca
    25021 gtgagcggtt tgtccggcaa ttttcttcgg attgccctga ttttgctggc gtagtcgagt
    25081 agagttttcg gcctgatccc tctttcggtg aggattcttt catatcggtc aagccacaca
    25141 tgaagagtga ttgcgtcacc gcctttaatt ctgtctatca gtgatttgcg tccgctgtct
    25201 gagagtaact caatattggc ctgtattgct tcagtgattg ctatcctcct gtctcggcct
    25261 aatccaaact ctttacccgt ccttgggtcc ctgtagcagt aatatccatt gtttcttata
    25321 taaaggttag ggggtaaatc ccggcgctca tgacttcgcc ttcttcccat ttctgatcct
    25381 cttcaaaagg ctacctgtta ctggtcgatt taagtcaacc tttaccgctg attcgtggaa
    25441 cagatactct cttccatcct taaccggagg agggaatatc ctgcattcgc gcacccatcg
    25501 acgaactgtt tcaaggcttc ttgggcgtcg ctggcgtgca ttccactcct gaagtgtcaa
    25561 gtacatcgca aagtctccgc aattacacgc aagaaaagcc gcattgatgc ggcgatggta
    25621 ggtctggata tcattgagcc atgaacaggc ctcatcgagt gtgaggctgt ggttagtcct
    25681 ttcgtaactc gctgattctc ctgtaagtct ctggtgcttt gtttccgtgt atcttcattt
    25741 cagacttcaa cagagcaacg agggaatccc attcgttgag gatgcctttg aatgccggaa
    25801 cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatcttctca acaagtgcaa
    25861 cgcatcgtcg gaagtctgct gcgtcatggg gagcgccgaa gtgatgacca tagatattct
    25921 ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
    25981 attgccattt caaaaccttc atagacaaat ctgacatttc ttgtctccaa taaaaaaaac
    26041 ctccatcagg cggcttggtg ttctttcagt tcttcaattc gaatattggt tacgtctgca
    26101 tgtgctatct gcgcccatat catccagtgg tcatagcagt cattgatgtt ctctgcttcg
    26161 ataactctgt tgaatggttc tccattccat tcacctgtga ctcggaagtg catttatcat
    26221 ctccataaaa caaaatccgc cgtagcgagt tcagataaaa gaaatccccg cgagtgcgag
    26281 gattgttatt cattgccgat attcaccttt atcgcgaaca cctttaccgg tttatcaccg
    26341 aagtgcggat gtgtgattgt cttgatttca tatccgtcat acggaacatc aattctgcgg
    26401 ctggaatcgt cgcgcttcgg atatcccttt gtgataatca ggcggtcata ctcccggaac
    26461 ataattcgct tattccaata gtcattacac aggcgatact cttccgtttt ctctcctcga
    26521 atcatggcat cgaagtattc acctttgacg gcaagttgta ggttagccac ggttaacctc
    26581 ctgcggagta gcctttacaa gcactggtgc aaatccatct tcattaaggt tatgaatata
    26641 gacttctgtt ctcctgcgct cttcaacgtt taatattgtt tctggatcat aaacccatac
    26701 tttcattcga ctattccatg aagaaatcgc ttcagatcta gttcgttttt ctggtccttg
    26761 ggcaccacat ttgcatgaca catagcgcat tttccctctg atactgaatg agtatccgat
    26821 gttaagcaca gtggaaccac agaatgggca gcgatataga ttcataaatc atccacctta
    26881 ggcgcagctg agacagcaac attccagaac tcagaaaaca tattgtatgc accagataga
    26941 ttggaagtag cataccctcc aagctcactt ctaatctgaa cggctctcat catttccggg
    27001 gttaactcct tcggcaccat aacccaacca tccggagtta ccggagagtt gccaccggga
    27061 atattttccg gaatattttg ttgtgcgttt tgtggttgtt ctgatttacc ctgaagcatg
    27121 gcggcgcggc aggcgttcca gccagctgtt cgcccaagcg cgtaaacttc agatggatca
    27181 agataatcaa tgtcatgccc gtcctcatcg tcgttctcag gtaatgcagc aggtactacc
    27241 ggtactggcg gagcggcgta gacctcaata attccattat caataggcca ttctccatcc
    27301 ttgaggtagt cacttgtgcc gtcaacttgc tgttctgcaa tgtggaatgc acctattggt
    27361 tttgcttcca gcgatgccag agcaatccgt gccagttcca tttgttcgcc actagtaagt
    27421 ccgttatcaa gcggggattt aatgaacaat tcgatacgtt ctttagtgat agtgctcata
    27481 tcactctcct ttgatgcgaa tgccagctgc gcttgctgat tcttcatatg cgcgtttagc
    27541 agcgttaagg attgctgcca gtggcgtata gccgccatcc attcggattg tgttgtggat
    27601 gccagccatt gtgtcgcgca atttgctgtg gctagcagac agttctgcta tgcacttctc
    27661 tgcggcatcg gctcgatctt tttcacgctg gaacattttg tgagtgcggt ctatagcttc
    27721 gattcgtgcc gctgatgtct tctgcacggc ttcaagctca acacgcagct tcccaaccgt
    27781 aagagcaata tcctcgttct tctggtcgcg gcgtttgatg tattgctggt ttctttcccg
    27841 ttcatccagt agtgccagca cggtttctgg cccggtcaga aatttgaagg cgttgagcgc
    27901 atcaatatcc acaccgtaat ccttaagttc ctgttcagtt aacaaatcat catcaactgg
    27961 caacattaac aggcgttcca ttgccggaat tgcacgctct gccgcctcac gcagtgcctg
    28021 atagtcaatc ttgctcattg gatgactcct ttacgaagct gttcggcgat atcttcgaga
    28081 acgccatcag agaatgaacg gtcaaaatcg ccttccggcg cattggccat aaactcagta
    28141 gaggtaagaa tcatccgggc aatatcagcg gcgttcttcg cagtatcatc aataaaacca
    28201 gcttcccagg cagccagcat tctgttcgcc acaaagtaag cgccctcctt gcgtgcttca
    28261 gtcttcactt cagccagaaa agcatcggtg gctggggttt cagtaatatc atcttcccat
    28321 tcgctaaact cctcacgaca aaagtcatta aattccttct cagattgctt aagcgaggaa
    28381 ttttcagcag ccatcttcgc gcatttagcc tcaaggttat caatcgtgat tccagcagaa
    28441 cgacactccc gcaacgccgt ttctagtttt gattcaagtt caccgaactt acggacaaga
    28501 tattcatcgt ttgtttcgtc aacctttaaa tcacttggga tgcatttacc tttcagaaaa
    28561 ccatccatct caattagtgt catttgtttc atttcttccc actccgccac atcgcattca
    28621 gatatttgtt gtcattaaca gaaccgaaac tctttctctt aagcaattcc tctctcgatg
    28681 gcattggctt t
//
LOCUS       DAZFDP010000048        28516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFDP010000048 DAZFDP010000000
VERSION     DAZFDP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..664
                     /gene="zapC"
                     /locus_tag="ACHJ1G_002173"
     CDS             122..664
                     /gene="zapC"
                     /locus_tag="ACHJ1G_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4764023.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(661..1770)
                     /gene="ycbX"
                     /locus_tag="ACHJ1G_002174"
     CDS             complement(661..1770)
                     /gene="ycbX"
                     /locus_tag="ACHJ1G_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4764024.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2014..4122
                     /gene="rlmKL"
                     /locus_tag="ACHJ1G_002175"
     CDS             2014..4122
                     /gene="rlmKL"
                     /locus_tag="ACHJ1G_002175"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4764025.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4134..6041
                     /gene="uup"
                     /locus_tag="ACHJ1G_002176"
     CDS             4134..6041
                     /gene="uup"
                     /locus_tag="ACHJ1G_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4764026.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6171..7424
                     /gene="pqiA"
                     /locus_tag="ACHJ1G_002177"
     CDS             6171..7424
                     /gene="pqiA"
                     /locus_tag="ACHJ1G_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4764027.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7429..9069
                     /gene="pqiB"
                     /locus_tag="ACHJ1G_002178"
     CDS             7429..9069
                     /gene="pqiB"
                     /locus_tag="ACHJ1G_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4764028.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9066..9629
                     /gene="pqiC"
                     /locus_tag="ACHJ1G_002179"
     CDS             9066..9629
                     /gene="pqiC"
                     /locus_tag="ACHJ1G_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4764029.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            9885..10052
                     /gene="rmf"
                     /locus_tag="ACHJ1G_002180"
     CDS             9885..10052
                     /gene="rmf"
                     /locus_tag="ACHJ1G_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4764030.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10122..10640)
                     /gene="fabA"
                     /locus_tag="ACHJ1G_002181"
     CDS             complement(10122..10640)
                     /gene="fabA"
                     /locus_tag="ACHJ1G_002181"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4764031.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10709..12469)
                     /gene="ycbZ"
                     /locus_tag="ACHJ1G_002182"
     CDS             complement(10709..12469)
                     /gene="ycbZ"
                     /locus_tag="ACHJ1G_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4764032.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12655..13107
                     /gene="matP"
                     /locus_tag="ACHJ1G_002183"
     CDS             12655..13107
                     /gene="matP"
                     /locus_tag="ACHJ1G_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4764033.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13182..14222)
                     /gene="ompA"
                     /locus_tag="ACHJ1G_002184"
     CDS             complement(13182..14222)
                     /gene="ompA"
                     /locus_tag="ACHJ1G_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4764034.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14579..15088)
                     /gene="sulA"
                     /locus_tag="ACHJ1G_002185"
     CDS             complement(14579..15088)
                     /gene="sulA"
                     /locus_tag="ACHJ1G_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4764035.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15307..15936
                     /gene="sxy"
                     /locus_tag="ACHJ1G_002186"
     CDS             15307..15936
                     /gene="sxy"
                     /locus_tag="ACHJ1G_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4764036.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15899..18061)
                     /gene="yccS"
                     /locus_tag="ACHJ1G_002187"
     CDS             complement(15899..18061)
                     /gene="yccS"
                     /locus_tag="ACHJ1G_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4764037.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18071..18517)
                     /gene="yccF"
                     /locus_tag="ACHJ1G_002188"
     CDS             complement(18071..18517)
                     /gene="yccF"
                     /locus_tag="ACHJ1G_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4764038.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18640..20694
                     /gene="helD"
                     /locus_tag="ACHJ1G_002189"
     CDS             18640..20694
                     /gene="helD"
                     /locus_tag="ACHJ1G_002189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4764039.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20726..21184)
                     /gene="mgsA"
                     /locus_tag="ACHJ1G_002190"
     CDS             complement(20726..21184)
                     /gene="mgsA"
                     /locus_tag="ACHJ1G_002190"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4764040.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21280..21942)
                     /gene="yccT"
                     /locus_tag="ACHJ1G_002191"
     CDS             complement(21280..21942)
                     /gene="yccT"
                     /locus_tag="ACHJ1G_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4764041.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22115..22528
                     /gene="yccU"
                     /locus_tag="ACHJ1G_002192"
     CDS             22115..22528
                     /gene="yccU"
                     /locus_tag="ACHJ1G_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4764042.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22573..22890)
                     /gene="hspQ"
                     /locus_tag="ACHJ1G_002193"
     CDS             complement(22573..22890)
                     /gene="hspQ"
                     /locus_tag="ACHJ1G_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4764043.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22948..24138)
                     /gene="rlmI"
                     /locus_tag="ACHJ1G_002194"
     CDS             complement(22948..24138)
                     /gene="rlmI"
                     /locus_tag="ACHJ1G_002194"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4764044.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24233..24511
                     /gene="yccX"
                     /locus_tag="ACHJ1G_002195"
     CDS             24233..24511
                     /gene="yccX"
                     /locus_tag="ACHJ1G_002195"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4764045.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24508..24837)
                     /gene="tusE"
                     /locus_tag="ACHJ1G_002196"
     CDS             complement(24508..24837)
                     /gene="tusE"
                     /locus_tag="ACHJ1G_002196"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4764046.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24928..25587)
                     /gene="yccA"
                     /locus_tag="ACHJ1G_002197"
     CDS             complement(24928..25587)
                     /gene="yccA"
                     /locus_tag="ACHJ1G_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4764047.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25995..27014)
                     /locus_tag="ACHJ1G_002198"
     CDS             complement(25995..27014)
                     /locus_tag="ACHJ1G_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764048.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26992..27234)
                     /locus_tag="ACHJ1G_002199"
     CDS             complement(26992..27234)
                     /locus_tag="ACHJ1G_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4764049.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27302..>28516)
                     /locus_tag="ACHJ1G_002200"
     CDS             complement(27302..>28516)
                     /locus_tag="ACHJ1G_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4764050.1"
                     /translation="KALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKV
                     TADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQE
                     SEQNDQKTEQNSPNAQQNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFD
                     EENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTS
                     RAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTI
                     KWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRS
                     YERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSA
                     IWQKLLPSQADF"
BASE COUNT         7169 a         6954 c         7413 g         6980 t
ORIGIN      
        1 tggttgtttt atattcatcg ctgttgctta tttagacatt ttgtactttt attgttgagg
       61 ttattaagcg aagcgacaat ggattgtgtt gctgcggttt tatagggtag gggagaggca
      121 gatgcgaatt aaaccagacg ataactggcg ttggtattac gatgaagagc acgatcgaat
      181 gatgctcgat ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca agatgttgac
      241 tccagatgcc ttttctcccg caggcttttg cgttgacgac gccgcgctct atttctcttt
      301 tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa aaagccgaac tggtgctgaa
      361 tgcactggta gcaattcgct atctcaaacc gcaaatgccg aaaagctggc attttgtttc
      421 ccatggtgaa atgtgggtgc ccatgccggg cgatgcggcc tgcgtttggt tgagcgatac
      481 tcacgagcag gtcaatttgc tggtcgttga atctggcgaa aatgccgcac tatgcctgct
      541 ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg ccattaaaat
      601 catgaacgac aggctgaaac cgcaggttaa tgttgacagc ttcagcctcg aacaggcagt
      661 ctaacgcgcc aacttaagtg cagtcttcgg tacacagcta cagcaaagaa tggtgccatc
      721 atcgcccatt gctgattttt ttagcggcgt gacttcgcct tctaaaagct gaacacggca
      781 acttccacaa atgcccgcgc ggcaagaata agggatacga attccctgat tttctaattg
      841 ctccagcaac acctgttggt tatttccacg aaatgcctgt ccctgccagt caatatctac
      901 atttgcgtcc ggttgttgcg tgatgttggc ggtatcatca gcggcagctg cgccgtaaat
      961 ttttgccgga gccgttgcca gaatttccac ctcatcgcca acgcgaatca cgccgctatt
     1021 acgggcaatt aaattctgac caaaatcgac atcgccgtta tcctgggcag tgcggaaaga
     1081 ttgcaatgtt tttaatggtt cgcctgccgg atgtttttgc cctttttctg ggctgacggt
     1141 ggtgaaaata cagcggctac aaggtttaac cacatcaaac accacatcac caatgcgaat
     1201 cactttccag cgatcttctt cccaggctga cgcgccggaa accaccagat tggggcggaa
     1261 ttgctccatt tttacactgg ccggacaacg ttgttggaga tcacgtaacg aggcttcgtt
     1321 agcaagaagg taaggatagc catcagcaaa tgacagaggt acagtgttgt ggcgtttcac
     1381 gcgccgggtc atttgtggcc ccacccagcg taattgcact tcgcgggaga aaaatccact
     1441 tagccatttg ttgatcgcgc ctggcgcaat tcgcgcggta aaatgtgtgc cccaaacttc
     1501 ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac tgccatctgg
     1561 tgcggtgaga tgcaagccat catgcacagg tgaaggagta aaccgtacca tttgcggaaa
     1621 ctggcgagca gtaataaacg taccatcagg ttccgtgatc ataaagatgc gatcgaaggc
     1681 cagaccactc acatctgcca gggtatgtgt aagaccaatg ccgcgcatcg atttaacagg
     1741 atgaataaaa agccgggtta atgtcgccac ggtgcggtcc tcaaatgaaa ataagccctc
     1801 aactttatga catgacgcgc ttattagcta taatgcgcaa caattttctt agcgcctgtc
     1861 ccgttaggct attttatttg tcagtttggc ccagggaagt gctcgaaatc ctcacgtact
     1921 tgtgtacgct ccggtttctc cgcgctgtcc atgtccagac tgactgcaac aattacgcct
     1981 actgcggtag gctcttagag taaaagtgac gatatgaatt ctctgtttgc cagtacggcc
     2041 cgtgggctgg aagagctgtt aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag
     2101 gtggttcagg gtggggtcca tttcaagggc gacacacggc ttgtttacca gagcctgatg
     2161 tggagccgcc tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt ttacagcgat
     2221 ttagacctct atctcggtgt tcaggcgatc aactggacag agatgtttaa tcctggcgcg
     2281 accttcgctg tccacttcag tggtttgaat gacaccatcc gcaacagtca gtacggcgcg
     2341 atgaaagtga aagacgcgat cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat
     2401 gttgatcgcg atgcgccgga tatccgcgtt aacgtctggc tgcataaaga aaccgccagt
     2461 atcgctcttg atctgagtgg tgatggttta catctgcgtg gctatcgcga tcgtgctggt
     2521 attgcgccga tcaaagaaac cctggcagcc gctattgtga tgcgatccgg ctggcagcca
     2581 ggaacaccgc tgctcgatcc gatgtgtggt tccggtacgt tgctgattga agcggcgatg
     2641 ctggcgaccg atcgcgcacc aggcttgcac cgtgggcgtt ggggctttag cggctgggcg
     2701 cagcatgatg aagctatctg gcaggaagtg aaagcggaag cgcaaactcg cgcccgtaaa
     2761 ggcctggctg agtatagctc tcatttttac ggttcggaca gcgacgcacg ggtgattcaa
     2821 cgtgcacgca ctaacgcccg tcttgcgggg attggtgaac tgatcacctt tgaggtgaaa
     2881 gatgtcgcgc aactgaccaa tccgctgccg aaagggccgt acggcacagt gttgagcaac
     2941 ccgccatacg gggaacgtct ggacagcgaa ccggcgctga ttgcgctgca tagcctgctg
     3001 ggtcggatca tgaaaaacca gtttggtggc tggaatctct ctttgtttag tgcctcgccg
     3061 gatctgctaa gctgcttgca gctgcgtgca gacaaacagt acaaggcgaa aaacggcccg
     3121 ctggactgcg tacagaaaaa ttaccacgtt gccgaaagca ctccagacag caaaccggcg
     3181 atggtagcgg aagactacgc caaccgcctg cgtaagaacc tcaaaaaatt cgagaagtgg
     3241 gctcgccagg aaggaattga atgttaccgc ctgtatgacg ccgatctacc ggaatataac
     3301 gttgccgttg accgttatgc cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact
     3361 attgatgctc acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac catttcggta
     3421 ctggggattg cgccaaacaa actggtgctg aaaacccgtg aacgccagaa gggcaaaaat
     3481 cagtaccaga aactgggcga gaagggcgag tttcttgaag ttaccgaata taacgctcac
     3541 ttgttggtga acctgacgga ttacctcgat accggtctgt ttctcgatca ccgtatcgcc
     3601 cgtcgtatgc tcggccagat gagcaaaggc aaagatttcc tcaacctgtt ctcttatacc
     3661 ggcagcgcca ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac cgtggatatg
     3721 tcgcgtactt atctggagtg ggcagaacgc aacctgcgtc tgaatggcct gaccgggcgt
     3781 gcgcatcgcc tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttc
     3841 gatctgatct ttatcgatcc gccaaccttc tctaactcaa aacgaatgga agatgcgttt
     3901 gatgttcagc gcgatcatct ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt
     3961 gggacgatca tgttctcgaa caacaaacgt ggcttccgta tggatctcgg cggcctggca
     4021 aaactgggac tgaaagcaca agaaattacg caaaaaacgc tctcccagga tttcgcccgt
     4081 aaccgccaga tccacaactg ctggctgatt accgcagcct gaaaggaata gtaatgtcat
     4141 taatcagtat gcatggcgcc tggctgtcgt tcagcgacgc gccgcttctc gataacgcag
     4201 aactgcatat cgaagataat gaacgtgttt gtctggtagg ccgcaacggc gcaggcaaat
     4261 cgacgttaat gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc attatttacg
     4321 agcaagattt gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt gagggtagcg
     4381 tttatgattt cgttgccgaa ggcattgaag aacaagcgga atatctgaaa cgctatcacg
     4441 atatttcgcg cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg
     4501 ttcaggaaca gctggatcac cacaacttgt ggcagctgga aaaccgcatc aacgaagtgc
     4561 tggcgcaact ggggttagat cctaatgttg cgctgtcgtc gctttccggc ggctggttgc
     4621 gtaaagcggc attaggacgc gcgctggtga gcaatccgcg cgtattgctg ctggacgaac
     4681 cgactaacca tctggatatc gaaaccatcg actggctgga agggttcctt aaaaccttca
     4741 atgggacgat tattttcatc tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca
     4801 ttgttgatct cgatcgcggc aagctggtga cctatccggg gaattacgac cagtacctgc
     4861 aggaaaaaga agaagccctg cgcgtggaag aactacaaaa tgccgagttc gatcgcaaac
     4921 tggcgcagga agaagtgtgg attcgccagg ggatcaaagc acgccgtacc cgtaatgaag
     4981 gccgcgtacg cgccctgaaa gcaatgcgtc gcgagcgtgg tgaacgtcgc gaagtgatgg
     5041 gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg taagatcgtt ttcgaaatgg
     5101 aagacgtttg ctaccaggtt gacggtaagc aactggtgaa agatttttcg gcccaggttc
     5161 tacgtggcga caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc acgctgctga
     5221 aactgatgct cggtcagctt caagcggaca gcgggcgtat tcacgttggc accaaactgg
     5281 aagtggctta tttcgatcag caccgcgcgg aactggatcc cgataaaacg gtgatggata
     5341 accttgccga aggtaagcaa gaggtgatgg ttaacggcaa gccacgccac gtattgggct
     5401 atttgcagga ctttctgttc catccgaaac gggcgatgac gccggtacgt gcgctttctg
     5461 gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc aacttattga
     5521 ttcttgacga accgaccaac gatcttgatg tcgaaacgct ggaactgctg gaagaactga
     5581 tcgacagcta tcagggcacg gtattgctgg ttagccacga tcgtcagttt gtcgataaca
     5641 ccgttacaga atgttggatc ttcgaaggcg gcggtaaaat tggtcgttat gtcggcggtt
     5701 atcatgatgc ccgtggtcag caagagcagt atgtggcgct caaacagcct gcggtgaaaa
     5761 aaaccgaaga agccgctgcg gcaaaagcag aaactgtaaa acgcagcagt agcaaactaa
     5821 gctataaatt gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat ctggaggcga
     5881 agctggaagc cctacagacg caggtggcgg atgcttcctt cttcagtcag ccacatgagc
     5941 agacacaaaa agtgctcgcc gatatggctg ctgcagagca ggagctggag caagcctttg
     6001 aacgctggga gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat agtcgattta
     6061 aacagagaca gctgacaacg gtaaaattgc ctgatgaact gggtttatca ggcatgaaaa
     6121 ccaacacttt gttagtcatc tgtaaaatag cgcatcatta aggagtacca atgtgcgaac
     6181 atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga catgctggtg gcgttaccgc
     6241 gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt
     6301 gggatgcccc tcggcagcgt cccaccgcct atgcgttggc tgcactgttc atgctgttgc
     6361 tgtccaactt gtttcctttt gtgaatatga acgttgcagg agttaccagt gaaattacat
     6421 tactggaaat tcccggcgtg cttttttctg aggactacgc cagcctcggc acctttttcc
     6481 tgttgtttgt gcaactggtt cccgcgtttt gtctgataac cattctgtta ctggtgaatc
     6541 gcgcggaatt accggtccgt ttaaaagagc aactggcacg ggtgcttttt caactcaaaa
     6601 cctggggaat ggcggagatt ttcctcgctg gtgtgctggt cagtttcgtt aaactgatgg
     6661 cttacggcag cattggggta ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc
     6721 aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg ggacgacatc gccccgatgc
     6781 cagaactgcg ccagccgcta aaaccaggcg tcacggggat acgtcagggg ctgcgttcgt
     6841 gctcctgttg tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt tgtagtacca
     6901 aagggtacgt tcggcgtaga aacagcctgc agtggacact cgcgctgctt gtaacgtcca
     6961 tcatgctgta tcttccggct aatattttgc ccatcatggt gacggattta ttaggctcga
     7021 agatgccgtc gacgattctc gctggggtca ttctgttatg gagcgaaggc tcttatcccg
     7081 tcgctgcggt gatctttctg gccagtatta tggtgccaac gttaaagatg atcgccatcg
     7141 cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga atgcatttga
     7201 tttatgaagt tgttgagttt gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg
     7261 tgctctcggc gctggtgcgt atgggaggtt taatgagtat ttatccggca atgggtgcat
     7321 taatgtttgc tttagtcgtc ataatgacaa tgttttctgc tatgacgttt gacccgcgtt
     7381 tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc ctgacagtat ggaatctaat
     7441 aatggggaag ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat atttcctatc
     7501 gtcacggcgc tcattggggc ctgggttctt ttttatcatt acagccatca gggaccggaa
     7561 gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac cattaaaagc
     7621 cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg ctgatgattt gacgcacgtt
     7681 gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga caccgtcttt
     7741 tgggtggtga aaccgcagat tggtcgagaa gggattagcg gtctgggaac cctgctgtct
     7801 ggggtttata tcgaactgca gccaggcgcg aaaggcagca aaatggataa atacgatttg
     7861 ctggactcgc caccgttggc cccgcctgat gcgaaaggta tccgtgtgat tctcgatagc
     7921 aaaaaagccg ggcagctctc gccaggagat ccggtgctgt tccgtggcta tcgggtaggt
     7981 tcggttgaaa ccagcacctt cgatacacaa aaacgcaata tcagctatca actgttcatc
     8041 aatgcacctt atgaccgact ggtgaccaac aatgttcgct tctggaaaga tagtggcatt
     8101 gcggttgatc tgacgtcagc agggatgcgt gtggagatgg gctcattgac aacgctgctg
     8161 agtggcggtg tcagctttga tgtgccggaa ggtctggatt tagggcagcc agtggcaccg
     8221 aaaacagctt tcgttttgta tgatgatcag aagagcattc aggattcgtt gtacaccgat
     8281 cacattgatt atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc gggagctccg
     8341 gtagagttcc ggggtattcg cctgggtacc gtaagcaaag tgccattctt tgcgccgaat
     8401 atgcgtcaga catttaacga tgattaccgt attccggtac tgattcgtat cgagccagag
     8461 cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg
     8521 aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg tcactggtgc actgtatgtt
     8581 gatctcgatt tctatccaaa tacgcctgca ataaccggta ttcgtgaatt taatggttat
     8641 cagattatcc cgaccgttag cggcggcctg gcgcaaatcc agcaacgact gatggaagcg
     8701 ttggataaga tcaacaaact gccattgaat ccgatgattg aacaggcaac cagtacgctt
     8761 tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc tggatagcat gaacaagatc
     8821 ctcgctagcc agtcgatgca gcagttgccg acggatatgc agtcaacgtt gcgtgaattg
     8881 aatcgcagca tgcagggctt ccagcctggc tccgcagcct acaacaagat ggtggcggat
     8941 atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac gctcaatgag
     9001 aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag atccagagcc gaagagggcg
     9061 aaacaatgaa aaagtggcta gtgacgattg cagcactgtg gctggccgga tgcagctccg
     9121 gcgaaattaa taaaaactat taccagttac ctgtggtgca gagcggtaca caaagtaccg
     9181 ccagccaggg caatcgtctg ttatgggtag agcaggtcac tgttcctgac tatctggcgg
     9241 ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt gattgccaat aacaacttgt
     9301 gggccagccc gttggatcaa cagttgcgca acaccctggt tgccaacctg agtacgcaac
     9361 tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta
     9421 ccgtcacgga gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt
     9481 tgaaccacca gggacaactg atcaaacgtc cgttccgtct ggaaggggtg caaactcagg
     9541 atggttacga tgagatggtt aaagtactgg ccagtgcctg gagtcaggaa gccgcttcta
     9601 ttgcgcaaga gataaagcgt ctaccttaat tataaagatt tgtaaatata accgcctccg
     9661 gtatgttgcc tgaggcggtt tttttgtctc taacgtgcgg aaaaagttgt tcctcttcac
     9721 attttttgta caaccggcat acccgtgtag ctcacaaata tgacagtggc gtgaattttg
     9781 cgcattgacg gcagttatga ttcgcggtat tgcttaactg tgattgcaca tttagtaatc
     9841 actgttttct tttccaccag aaaccagtat gagggaaacg aggcatgaag agacaaaaac
     9901 gagatcgcct ggaacgggca catcaacgtg gttatcaggc cggcatcgcc ggacgctcaa
     9961 aagaaatgtg tccctatcag acgctgaatc aaaggtcaca atggctggga ggctggcgag
    10021 aagccatggc ggacagggta gtaatggcct gattctgtct ctttaaaaag aaacctccgc
    10081 attgcggagg tttcgccttt tgatactctg tctgattata atcagaaggc agacgtatcc
    10141 tggaacagac cgactttcag gtcgctggcg gtatagatca gacgaccatc aaccagcact
    10201 tcgccatccg ccaggcccat aatcagacga cggttaacaa tgcgtttaaa gtgaatacgg
    10261 taggtcactt ttttcgctgt cggcagtacc tgaccagtga atttcacttc gccaacgccc
    10321 agcgcgcggc ctttaccttc gccgcccagc cagccgaggt agaaccctac cagctgccac
    10381 attgcgtcca ggcccaggca tcccggcata accggatcgc caataaagtg gcatccgaag
    10441 aaccacagat ccggattgat atccagttct gcttcaacat accctttgtc gaagttacca
    10501 cccgtttcgg tcattttgac cacacggtcc atcatcagca tgttcggtgc tggcaattgc
    10561 gggcctttag cgccaaacag ttcaccgcga ccagaggcaa gaaggtcttc ttttgtatag
    10621 gattcgcgtt tatctaccat gttctctgta agccttattt tattgaagca cgcaggatag
    10681 ctaacacgtg tacgctgaac aagtccgatc agttcggaat aaaccagttc agccaacgta
    10741 atggccatgg aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata cgttcctgga
    10801 tggtttgcat cagcgtcgtt tggccttcgc catcccacac cagatttaat aataacggca
    10861 gtgcgtcagt cacatcgtct actgcccaga tggtgaattt gccttcttct accgctttca
    10921 ccagttcact gtgaagactt aaatggcgaa cgttagctgt ggggataatg acaccttgtt
    10981 tcccggttaa ctcacgttgc tggcaaatag caaagaagcc ttcgattttc tcatttaaac
    11041 caccgaccgg ctgggcgcga ccgaactgat cgactgaacc tgtgatagcg atactctgat
    11101 tcaccggcac atcggcgagg gcgcttatca gggcgcagag ttcagccatc gaggcactat
    11161 ctccatcaac ttcactgtat gactgctcaa atgtcagcga tgctgagaag gggatctgtt
    11221 gctcaagctg tagttccgac atcaggaacg cttgcatgat catcatccct ttcgcatgga
    11281 tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca
    11341 caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga aattcaatga
    11401 ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct
    11461 gaaggatctc atcctgcata cgttcagcga ggaaaccttc gcgccattca cgctgctgca
    11521 gcattaagtt tagctgctcg ccggagaagg tgtcgccatc acacagggag gcgacctctt
    11581 tacactggcg gaggatccac tgcgggctaa gcggaagtgt ttcttgttca ccggtgtagc
    11641 gtgctgcttc gcggataagt accggccagg catccgctcc tggtgcaggt aagtgattat
    11701 gtctggcggt aaatgtcacc cagcgacacc actgggttac tgactctgca tcgacaatct
    11761 gcagagtatc ttcaaattcg ctataaatag cctgctctga aagctctggc tccatctcct
    11821 ggaaatcaga caatgattcg cgttcgccta ccagaatgac tttcagcttc aatggcatcg
    11881 aaggcacaga gacggggaga gggcgcgact catcaaacgc aacccagtca aaacgctcgc
    11941 ggttaacgat atttttcagc cgcatccaca gcagaggttg cgccagcagt gtacgcaaag
    12001 agatgatgag aataccgcca tttgcctgat gcaccagacc aggctgcagg gtaatgtcgc
    12061 cattaaactg gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg gcagcgacaa
    12121 cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag cggatggagt
    12181 ggccggaaac ctcatagtga ccgcccacca gttggcctgc atcgctttgt agcgtacgcg
    12241 cggcattggc aataagattc agatactcag actcttccgg ggccttcgcc agcataaaag
    12301 aggaggatgc tcgcgtatgc agcaactgct ccagtgcgaa ttgcagtcgc ggttgagtat
    12361 cactgaataa aggatcgttt tcgtcaatca aatgtggctg agcaaatatt tcctgatagc
    12421 tatcggtatc aggaaccagg tcacgccatg caagtttcgt aatggtcaaa gttgatgttt
    12481 tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc actacagcac acgtagaaag
    12541 caccgacaat actcctggca tgggcgttaa agctcacagg atggagattc ttttcttcac
    12601 tggcctaaaa agctgatatt ctgtaaagag ttacacggta acattgagat cgctatgaaa
    12661 tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga agtatctggt gaagaagcat
    12721 cgcgaagggg agttaatcac ccgttacata gaagccagtg ccgcccagga agccgtcgat
    12781 gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg gctggattga caagcatatg
    12841 aatccggaac tggtcaatcg catgaagcaa accattcggg caagacgaaa gcgccatttt
    12901 aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc tggaatttat tgtctggcaa
    12961 cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt ctgaaacgat tgttcagctg
    13021 attgaagatg cggaaaacaa agagaaatac gcgaataaaa tgtcttctct gaagcaggat
    13081 ctgcaggcat tgctgggtaa ggaataactg acgaaagtca gttcaattta ctaaaggcaa
    13141 aaaaaccccg cagcagcggg gtttttctac cagacgagaa cttaagcttg cggctgagtt
    13201 acaacgtctt tgataccttt aacttcgatc tctacgcgac gatccggagc caggcagtcg
    13261 atcagtgcag cacgctgttt cacgttgtca caggtgttgc cagtaaccgg gttggattcg
    13321 cccataccac gtgcggagat cttgtctgcc gggatacctt tggagatcag gtaatcaaca
    13381 acagactgag cacggcgctc ggacagaccc tggttgtaag cgtcagaacc gatgcggtcg
    13441 gtgtaaccca gaacaactac ggaaccgtct ttcggatcca ggttgctcag ctggctgtac
    13501 agctgatcca gagcagcctg accttccggt ttcagggttg ctttgttgaa gttgaacaga
    13561 acgtcagact tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc tggagccgga
    13621 gcaactactg gagctgcttc gccctgaccg aaacggtagg aaacacccag gctcagcatg
    13681 ccgttgtccg gacgagtgcc gatggtgtgt gcgtcaccga tgttgttggt ccactggtat
    13741 tccagacggg tagcgatttc aggagtgatc gcgtactcaa caccgccagc gaagaccgga
    13801 gaaacgccgg tgtcgtggtt tttaccataa acgttggatt tagtgtctgc acgccatacc
    13861 ataccaccca gacgagtgta gatgtccagg tcgtcagtga ttgggtaacc cagtttagcg
    13921 gtcagttgaa cgccctgagc tttgtatgca ccgttttcaa cgctgccttt gtacggcata
    13981 cgacctaacc agtcgtaacc catttcaaag ccaacatacg ggttaacctg gtaaccacca
    14041 aaagcaccag cgcccagttg gttttcatgg gtcgggccat tgttgttgat gaaaccagtg
    14101 tcatggtact gggaccagcc cagtttagca ccagtgtacc aggtgttatc tttcggagcg
    14161 gcctgcgcta cggtagcgaa accagccagt gccactgcaa tcgcgatagc tgtctttttc
    14221 attttttgcg cctcgttatc atccaaaata cgccatgaat atctccaacg agataacacg
    14281 gttaaatcct tcaccggggg atctgctcaa tattaactct accgatatct tcggcttatg
    14341 ccgagcaccc ctggcgatgt aaagtctaca acgtagttga aaacttacaa gtgtgaactc
    14401 cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt taatcttttt tgtcagcgac
    14461 aatttacaga agaaaatcgc ggaaaccgct tcagacaagc ctccgcaagg aaaattagtc
    14521 acgactgaaa gcattggctg ggcgacaaaa aaagttccag gattaatcct aaatttactt
    14581 aatgatacaa attagagtga atttttagcc cggaaagttg tctcgtggcg tgagaggatg
    14641 cgcttaccgg acgcataata aaccccatag cgttaccttc atttgccgca tcaacaagtt
    14701 cagcatgctc ttcttcagtc aaatcatctg ccaaccaacc gatcaccaca ctgtaattgc
    14761 ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca aggggagagc tggctaatct
    14821 gcattacttt cgttaagggt agcccagatg cctgaaccca ttcccgactc agtttttgtt
    14881 gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca
    14941 gtagaagttg cgtcatcatg ggctgatctt cgcgatagac aacttcactg ataagcccgg
    15001 ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc tgcggatgag aacgacgaag
    15061 aacgatgtgc atagcctgaa gtgtacataa tcaatccagc ccctgtgagt tactgtatgg
    15121 atgtacagta catccagtga caacaaagat caaccctatt ttcggaaaga gcctcgcaaa
    15181 ttttgtcgtt ggtgacggga aaacataaat taatcttgcc ccttaagaat aagttgccta
    15241 ttttcgtagt taacggatcc gttaatgtga atcattcttt tatgttatga ttttaaaagg
    15301 aattttatga aaagcctctc ctataagcgg atctataaat cacaagaata cctggcaacg
    15361 ttgggcacaa ttgaataccg atcattgttt ggcagttaca gcctgaccgt tgacgacacg
    15421 gtgtttgcga tggtttctga tggtgagttg tatcttcggg cttgtgagca aagtgcacag
    15481 tactgtgtaa aacatccgcc tgtctggctg acatataaaa agtgtggccg atccgttacc
    15541 ctcaattact atcgggttga tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg
    15601 tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga
    15661 ctgaaagatt tgcccaatat gtcttttcat ctggaagcga ttctcgggga ggtggggatt
    15721 aaggatgtac gggcgttacg tatacttggg gcaaaaatgt gttggttgcg actgcggcag
    15781 caaaacagtc tggtgacaga aaagattctg tttatgcttg aaggtgccat tatcggcatt
    15841 catgaagctg cgctcccggt ggcacgccgc caggagcttg cagaatgggc tgactctctt
    15901 acgccgaaac aggagtttcc tgcggaactt gagtaatctg gcgttgcaga cgaccaatct
    15961 caggcagtaa tgcaatcaat aatccaactt gttgtacgac cagaggttct ttgctgtctg
    16021 cccgtggttc aagttgctgc atccgctgtt tcaggctagc taatgcctca ttgacgcgtt
    16081 cttcatcagc aggttgatga tgtaacgcgt catcaacata gcaaactgcg tcatcaagaa
    16141 acgccagaat ttcaggatta gttaactgct cccggtgagc accgagggct gagatatagc
    16201 tggtaaacgt atggttaagg cacagcaacc gaaacgcggc ttcgcgaatt tgcggggtaa
    16261 cgttcggctc gctggacata tttgatacca ccgacgccag ctcagcatca cggttgtgtg
    16321 catcgcggcg ggcaatacga tacgccagac ggttatcacg cccctgatgg tattgctcca
    16381 gtatggcatc gagataccga cagttggcct ctgtggcgcg ttcgagcatg cgcggcagat
    16441 tgcgaaactg ccagtcaggc cagatgtagc tcactgccgc ccacgcaatg gcacaaccaa
    16501 tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc ttcacccagt aagttaaaac
    16561 acagtagcac caaaagtgtg atgaacatcg ttgcatgagc gtattgcacg ttacggaagg
    16621 caaaaaagag cacgccggta ataaccagca gcaccagctg cccttccagt gatggcacaa
    16681 accacagcac aggaatgcca atggcgatac ctaccagcgt accaataatc cttaacttca
    16741 ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa caaacttgtc agcaagatcc
    16801 aatacccgtg atgcattccg gttatttgaa tgatggcgta gccgaagcac aacaccagcg
    16861 acattcttac cgcatgacgg aagagggcgg attccggcgt gaagtgacgg ctaagacgca
    16921 gccagatatc actcaacccg tgcgggctgt catcagcgag ctcattttcg tcattattat
    16981 ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag ttgggcatca atggcgcgta
    17041 aattgttcag caaaaatccc agtgttttga gtaaatcggc gggtgcgccg ttatcgcgca
    17101 tccgctccag cgcagcatca atatgcgtaa aagcgcgctc aaaatgcgga tcatgttgat
    17161 aaggctgacg caacaaaata cagcgtgaca gttgctggca cgcctggccc tgcatcgaca
    17221 tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa atgttcacgc aatgtttgat
    17281 actgaatatg agaagagctg gcacgctcgt gaatatcctg tgcgacaaag taataatgca
    17341 gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa gcgggtcagc agcgagagtt
    17401 tcgtctgatt caatgtcgcc atcagcagac cgttggcgag agccaaatcg tacagcggtg
    17461 cctggctttc atcttcaata tcaggatcaa acatgcgcga cttgagctca agataacgcg
    17521 ccagttgttc atagcaacgc gccaggttgt cctgcagcgg gcggaccggg aacagcagat
    17581 gaccaataag tgtcaggacg ttgtaccaga cggcaccggc cagcagatac atcggctgct
    17641 gataccagtg ctcatacagt gatgttccca acatagtgta aatggcgatc agcaatgcac
    17701 cgaaggcaat tgttgcatag cgttgaccca gaccgccgag caaaatgaag ccgctggtag
    17761 agagcgttaa gccaatcgca aatagccagg gccagggaaa cagcaattct actgaggccg
    17821 aggcgataaa aaagcagaac agcgtaatga tgaggttacg caaacgtccc gccagtcggt
    17881 catcgagatc ggtcagcgcc gctgccacca tccccagcgt tagcggaatc gtcagtttta
    17941 catcacccag ccaccacgga aacgctgtgg ttccacaaag cgcaataaaa atacgcgcgt
    18001 aatacagcca ggcgctgttc caggtatagc gtttgagcaa aggacttagc ataaaggcca
    18061 tattagtgga ttattcaaaa cgacgacgtg cattggcttc acgcgcagct tgcgctgttt
    18121 ctaccgatac cacgcgacga ccaaccggcc atagtgcaat agcggcaatt ttaaagttcg
    18181 caatgccgac aggaatgcca atgattgaaa tacattgtgc gatgcccgtt gcaatgtgca
    18241 tcaggcataa ccaccagcca aagaaaatca gccagaaaat attcaatacc gtaccgccag
    18301 tattcagcag tacatttttg ccagccgggt tcagttcatc gacatgaata gcttcattgc
    18361 cataaggcac cagagacagt ttagtgatct cccagcagga tcgtgtcagc ggtaaggtaa
    18421 aaatcagcac aatactgacc agagtcgcca acagccagcc cagagtggtg gcaaatccgc
    18481 caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa acctcgcttt actgtggttt
    18541 tcagtaatgg gcggcaattg taacgttttt ttgggctgga gcacgttttc tctgacggtt
    18601 acactgataa gaaataattt cgtgtggatc tacagagtca tggaactgaa agcgacaacg
    18661 cttggaaaac gtctggcaca gcacccttac gatcgggcgg tgatcctcaa tgccgggatt
    18721 aaagtctccg gcgatcgcca cgaatacctt attcctttca atcaattact ggcgattcac
    18781 tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac tgccggacga aaaagtggtg
    18841 cgtctgcacg gcaccgaatg gggcgagacg cagcgttttt accatcatct tgatgctcac
    18901 tggcggcggt ggagtggcga gatgagcgaa attgcgtctg gtgttttacg ccagcaactg
    18961 gatttgattg ccacgcgcac tggggaaaat aaatggctga cgcgtgagca aacctctggc
    19021 gtgcagcaac aaatccgcca ggctttgtcg gcgttgccgt tgccggttaa ccgactggaa
    19081 gaattcgata actgccgtga ggcgtggcgt aaatgtcagg cctggttgaa agatattgaa
    19141 agcgctcggt tgcagcataa ccaggcgtat accgaagcca tgcttaccga gtatgcggat
    19201 tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc aggcccgggc agtcgttaat
    19261 ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg gaaaaacgtc ggtgctggtg
    19321 gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc ctgagcaaat tttattgctg
    19381 gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga ttcgcgaacg gctacatacc
    19441 gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc atattattca gcagggcagc
    19501 aaaaaagttc cgatagtcag caaactggaa aatgataccg ctgcccgtca tgaactcttt
    19561 attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc aggcgaaggg ctggcggcaa
    19621 tggctgacgg aagaaatgca gtggtcagtg ccagaaggta acttctggga tgatgaaaaa
    19681 ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa gtctgatgcg gatgcacggt
    19741 ggtgcacagg cagaaatgat tgccagtgca cccgaagaga ttcgcgatct gttcagtaaa
    19801 cgtatcaagt tgatggcccc gttattaaaa gcctggaaag gtgcgctgaa ggcagaaaac
    19861 gctgtcgatt tttcgggcct tattcatcag gcgattgtga ttctggagaa aggtcgcttt
    19921 atcagcccgt ggaagcatat tctggttgat gaatttcagg atatctcgcc gcagcgggca
    19981 gcgttgttag cggcattacg caagcaaaac agtcagacga cgttgttcgc cgttggtgat
    20041 gactggcagg cgatttaccg attcagcggc gcgcaaatgt cgctcaccac cgctttccat
    20101 gaaaactttg gtgaaggcga tcgctgtgat ttagacacga cttaccgttt taacagtcgt
    20161 atcggtgagg tggcaaaccg gtttattcag cagaacccag gccagttgaa aaagccgcta
    20221 aacagcttaa ccaatggaga cgaaaaagcc gtcacgttat tggatgagag tcaacttgac
    20281 gctttgctgg ataagctctc tggttatgcc aaaccggaag agcgcattct tatcctggcg
    20341 cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg caacacgctg gccgaagttg
    20401 caaatcgact ttatgaccat tcatgccagc aaagggcaac aggcggatta cgtcatcatc
    20461 gttggcttgc aggaggggag tgatggtttt ccggctgcgg cgcgggagtc gattatggaa
    20521 gaggcgctac tgccaccggt tgaggatttc ccggacgctg aagaacggcg gttaatgtac
    20581 gtggcgctga cccgggcacg ccatcgggta tgggcactgt ttaacaaaga gaatccctct
    20641 ccctttgtgg aaatactgaa aaatctggat gtgccggtgg cgagaaaacc gtaagaaaca
    20701 ggtggcgttt gccacctgtg caatattact tcagacggtc cgcgagataa cgctgataat
    20761 cggggatcag aatatcgacc gcgtcgttga aatgcggcga ctggataatg aagtctgccg
    20821 ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc cagacgcagc aaggctttca
    20881 cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa gaaaatcaat acatcaattt
    20941 tcccttctga gatcaatgcg ccaacctgct ggtcaccccc cattgggcca ctcaacatcg
    21001 cgttgacgtt catgccggtt gcacgggaaa tcaagttacc ggtagtgcct gttgcataca
    21061 gtacgtgttg ttccagtaac ggttgatgcc gttccaccca gctcatcagc atttgtttgc
    21121 agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg taaagtgcga gtcgtcagtt
    21181 ccataatgta catccgtagt taactttcct acagattact gtaagcactt atcgctgcaa
    21241 gataaagacc gaaaaagcct gcgcacaggc acaaaaatct caggaagatg gttgtttttc
    21301 cgcccactgc aggaaagtat ttcgcgtttg tgggtcagcc agtttaaacc aatacttcag
    21361 ccgttgttct gtgagcacct gagactgcgg tggaatagca tccagctcgg aaacacccga
    21421 aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc ggtagtgaag cgcgtttcgc
    21481 ggatttgtta tagcgttcaa cctcgacctc ataatcaatc gtttttgccg ttgaggtaat
    21541 ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg ccatccaaca attcaaggcg
    21601 cggcgcggca tcaaaatggt tagcttcccg ctcattctcc aggcgaggca ggcgaaaatt
    21661 gacctggttg atgagctggg tattaaaact gaccaccagc ggtggggaga tatacagccg
    21721 ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga aacactaact gatgcggtcc
    21781 attatccagt tcaatgctgt ccgcaccgcg cagcagagag ctggaaacct tcttgccgtc
    21841 gagtaccaga agatcgacat cggttgaaag ccgcagcgtg gtggcaaaaa cggataccgg
    21901 caaacataat gcaatcaagg tggtcacgat gccggttttc ataggtagct cctgtcaaaa
    21961 gaccgcccga ataataatgt gtcaaaattt ttctttaatc atacttacta acatatagac
    22021 atatttcccg cttttgctct cattcattcg tattagctgc atggttggca tgtcggcttt
    22081 gtcgtacact ttgttaaaca gtctggagga aataatgaaa gaaaccgata ttgcaggcat
    22141 tttaacgtcc actcacacca ttgcgctggt gggggcaagc gacaaacccg atcgtcccag
    22201 ctatcgcgtg atgaaatatc tactcgacca ggggtatcac gtcattccgg tttcgccaaa
    22261 agttgccggc aaaacgctgc tgggccagca ggggtatggc acgctggcag acgttccgga
    22321 aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg gcgtggggcg tggcacagga
    22381 agctattgcc attggcgcga aaaccctctg gatgcaactg ggggtgatta atgagcaagc
    22441 ggcagtactg gcacgggatg ctgggttgaa tgtggtgatg gatcgctgcc cggctattga
    22501 gatccctcgc ctgggcctgg ccaaataaaa aatccccgga aggcaaaaac cttccgggga
    22561 ttcgttcagg gatcagttac gcagacgcgg ggcctggagt tgtttgcgga tggtctgcgc
    22621 cagctcatcc atggatggct gttcaggatg ttcatcctgc aattcgctac ttagctgggc
    22681 ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc tccatcacca cgtgatacca
    22741 cggagcggcg cgaagctcgt cattcaccgc cagctcatca ggcgacggtt cagagagcga
    22801 ataaaccggg tcgatatcca cgaccactcc gagataacct aacagggaat ggcggacctg
    22861 ctggccgata ccgaatttgc tggcaatcat agtcacctcc cgggaaatct ttacctttga
    22921 tataagggta aaattccaca tttcaagtta catgacgcga caggcaaacc ctttcagata
    22981 tagcccttcc ggataggtag cgatcaccgg atgatcggct gcctgacgga actgctctat
    23041 aaattgtaca tcacggccgg catcaattgc ggcatccgcg atgattttct gaaataaatc
    23101 gctggtcatc agaccggaac aggagaaagt caggagaata ccgccttcat tcagcaactg
    23161 aatcgccagc atgttgatat ctttatagcc acgacacgcg cccatcaact ggcttttatt
    23221 ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat atttcaccgc gatcgcgata
    23281 agtacgcagc aatttaaaga catcatcacg gacaaactca gccttgctca gatccagttt
    23341 gttcagctca acgttctgcc gtgcaatatc cagcgcttcc tgggaggtat caacgctgac
    23401 aacctggctg caaccgccca tcagtgccga tacggcgaaa ccaccggtat aggagaaaca
    23461 gttcagcaca cgtttatttt caacgtagcg gcgggtagcc aggcggctat cacgctggtc
    23521 caggtagtag cccgttttgt gtccgtgctg aatatccacc agcagcttca tgccgtgttc
    23581 ttcaatcggc agcagggcag gcggcaactc gccggtgacg aggccctggg tcagctccat
    23641 cccttctttt ttacgtaccg caacatcgct gcgatcgtaa atcgcacatt ccgggtacag
    23701 cgtttgcagg gcactaacta atgccgcgcg ctgatattct gccccagcac tcagcagttg
    23761 cagcaccaga aaattaccga aacgatcgat agtaataccc ggcaggccat cagattctcc
    23821 ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc agccagtcac gccatttttg
    23881 tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata gactcagacg ggtcaaacgt
    23941 ccagacgcgc gcccggattt gcgaagctgg tgaataagcg ccgcgtgcta accattttcc
    24001 ctgatgatca acaatatcga tggtttcacc gaggctggct ttaccttcca tgcgggcaac
    24061 ggccccggaa aagacccacg gatggcgacg aagtaatgat ttttcgcgcc ctttggctaa
    24121 cactaaacgt acactcataa tttacttttc tgtcgatgca aagaaatggg cgtcattgtc
    24181 cggagttttc catggatttg caacgcgtcc attaaggata aggagagcag caatgtcgaa
    24241 agtctgcata attgcctggg tttacgggcg agttcagggc gtaggatttc gctacaccac
    24301 acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc aaaaatcttg acgatggcag
    24361 cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa aaattaatgc tgtggttaaa
    24421 gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc agcgagccgc atcatccctc
    24481 gggggaatta accgattttc gtatccgtta aatacatttt accggttttg gcaggccagc
    24541 aattttggtg gcttgcttag ccggaccttt cggaaacagt cggtataaat agcggctgtt
    24601 acctttttct tcgccaaatt tattcgccat cgcttttacc agcatacgaa tcgccggaga
    24661 agtattgaat tccagataga aatcacgcac aaaacgcacc acttcccagt gttccggtga
    24721 cagcgaaatc ccttcgttct ctgcaatcac cactgccagc ggctcactcc actggctgct
    24781 ttctttgaga tagccttcgg tatccgtttc tatctcttta ccttcgaaga tcagcataat
    24841 tattactacc ttaatcagac tggcggcagt gtaacaaaaa acaaagcccc gcataaagcg
    24901 aggctatgaa agtgttagcg ggtgagatta atcgcggctg gcgaagccca gaatgctcag
    24961 caggctgacg aagatgttgt acagcgaaac atacaggcta accgtggcac gaatatagtt
    25021 cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa atagcgccag aggagatcaa
    25081 aatgaagacc gcgctgatcg ccagatgcag agcaggcagc tgcaggaaga tattagcaac
    25141 cataccaatc agcaccacca caatacccgc catcagcata ccgccgagga acgacatatc
    25201 tttgcgggtg gtcagcacat atgcagagca gcagaagaac actaacgccg ttccgcccag
    25261 tgccatagcg attacgtcac ccattccggc agacagatag gtgttcagaa taggcccgag
    25321 gatataaccc agaaaaccgg taaaggcgaa tgcggagata atcccggtcg gcttattcgc
    25381 cgttttatag gtcaggaaca tcaaaccata catacccacc agcgtcagaa tcagacccgg
    25441 agatggcagc atcagcaccg tactggcagt tgcggtaatt gccgaaaagg ccagcgtcag
    25501 gctcagcaga aaataggtat tacgcagcac cttatgggtg ctaagcagtg atgtacggtc
    25561 atgtgaagaa ctaacaatac gatccattga gtcactctct atgacagatg taattaatta
    25621 agcagcataa tgataatgcg taagggcacc cagaagtttt acccatcttt acgcatttga
    25681 tctggaacag gtttaacagc ggattatcag gtcattaagc aaatataacg ccctgagaat
    25741 ttcgacaggc aaaagaaaaa ggggttagca tttagctaac cccttatctt atttggcgga
    25801 agcgcagaga ttcgaactct ggaacccttt cgggtcgccg gttttcaaga ccgaagaaaa
    25861 acaaataaaa atcagtgtaa tacgtgaaat tgctgaaaag ttaatcaaat ttaacaccaa
    25921 gaaaaatcaa ggtattagga agttgctcat agctagtata ccaagtcttt ttgccagaaa
    25981 tttttctgga ttgtctactt gctaaccacg agcggcaaat ccagtgttgg tgtgattttt
    26041 gttttacgat cgtaaatcaa cacctgattt tctgttttat gcccgctgaa aatttgtttg
    26101 tcgcgactgc tgccttcgta atctgagatc cccttggctt ttatgtcgtg aaaattacac
    26161 ccgaacggaa cgccaacttt ttgctcggct gcgcgtttag cctgattcca ccagttattc
    26221 agcgttttag ctatgacttt cccgcctttg gttgtattga tcacatattc gcatgtacag
    26281 gaagatacat ttcgggctaa ctggatcgct gtacgtaatc gcggtgacca ttccttgatt
    26341 tgtttggtgc cggttttgtt ttgctcaatg tagatccctt tatccataat atcctgccat
    26401 ttcaactcaa gcacatcacc gagtcttgcc gcacagagat aggaaatctc cattgcaatg
    26461 cgtaactgtg gaattgcttc cgcatatatc gccgcatact gttcatcggt gatgtaaaca
    26521 gtgcgggctt taagagagaa ttttctgact cctttgcatg ggttattctt cacataccca
    26581 cgctcatatc cccacccgta tactcgactc aggcttgcca gttcatgatt tgcctgggtc
    26641 ttgctctcaa gccctcgttt atccatgaaa attcttacct gctcagtttt gacattatca
    26701 gcaagcactt ttccgaatac catcagcaac gccttctgat gttgtcgata atctttttgg
    26761 gttcgggggg ccagttctgt aaatgcaggg gagtccataa acatgtgcca caatttagct
    26821 acggtcatta tgttgtggag ttttgctttt tccagttcat aattttgcca gactttagct
    26881 acgctggttt tccgcactct tcctagccct atacttcttg tacttccttc gggtttccac
    26941 acgtaactgt aaccattcga tctaacccgc ggtggcagta cattatcttt tttatttttt
    27001 cttggtcttc ccattgttca gcgcctcaaa atcgggttca gcagaaacca gttcagatgt
    27061 ttttggcatc gttgtcagac cgtgtggaat atccctgcgg agaacgatgg gttcgttttt
    27121 agggccgatt acaaatggga tgccgtgcag tcttaactgg tgttgctgtt ttgtgtatcg
    27181 ctcgtatttc gtgatctctt taatctctgc tggagataga gttaattcgt acatgtggtc
    27241 acgtgccttt acagcatgac cgccgccact atagctggtg ggcggcgata tgggttgaac
    27301 atcaaaaatc agcctgactc gggagcagtt tttgccagat ggctgaaacg tatttcgcct
    27361 ggtaacgtgc atcgttcaga gcgttatggc gttcaccttc gaatggaata gtcgttcttg
    27421 cgtcgaaatc catcaccagt cccagagcaa ccatcgttct tacatcgcga tcattggtgt
    27481 aacgccacgg gcaggggatc ccctgccgtt catatgaacg gcgtaaaatc acgttgtcga
    27541 aagttgcacc gttaccccag acctgaacaa aaaattcacc ggagttttcg tcgataaatt
    27601 cccgtaattg cagcagtgca tcatctaacg ggatttcatc ggtaagaatg gcggattgcg
    27661 cttcgcgtga ctgtttcagc caccacttaa tggtgtcccg atcaatgact ccgccagcag
    27721 tttccagatc gatagttttg ctgaattctg gccccatctc tccggtttgc ggatcgaaaa
    27781 atattgcacc tattgagata atcggggcat caggattttt tcccatggtt tcaaggtcga
    27841 tcatcagatg aatccccgct ctgctggtgg atgtgagatt atgatgaccg ttcgccttaa
    27901 ttaagggatc tgacgcctcg ccagtttcac tatcgctggc atgatgctga ttgccgccag
    27961 tgttctcctt gtgctgatgc gcagtgcctt ccatttcctc cggatcattt tcctgaactt
    28021 caggctgatt ctcttcatcg aatatttcct ggtatgttgc gtcacttatc accgcaccac
    28081 aatcagggca gctaccgccg ccggtctgac cgcaggcggt gcaggctttt tccggctcct
    28141 gtttcgtttc tggctcgttt tgttgcgcat ttgggctgtt ttgttccgtt ttctggtcgt
    28201 tctgttccga ttcttgctgg ttctggttca cagaatcgca ggtttcaatc cccttcaccc
    28261 atttcggatc attcggatcg ctaatccctt caacaaattc accacgtgat gcagcaagca
    28321 atttatcggc gtcaggctgg ctgatattgg ctgcctgcat aattttgttt acttcgtcag
    28381 cggtaacttt taccggctcc ggttgtacgg aatcttcagc ggtatccaca ttttgcggta
    28441 agtccgtgta tgttccgttt ttgcgggcaa gatattcttc tttcgtgatt tcagtagccc
    28501 cggcagccag cgcctt
//
LOCUS       DAZFDP010000049        28301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFDP010000049 DAZFDP010000000
VERSION     DAZFDP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..1148)
                     /gene="gldA"
                     /locus_tag="ACHJ1G_002201"
     CDS             complement(45..1148)
                     /gene="gldA"
                     /locus_tag="ACHJ1G_002201"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4764051.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1159..1821)
                     /gene="fsa"
                     /locus_tag="ACHJ1G_002202"
     CDS             complement(1159..1821)
                     /gene="fsa"
                     /locus_tag="ACHJ1G_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4764052.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1833..4334)
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_002203"
                     /pseudo
     CDS             complement(1833..4334)
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4643..5722
                     /gene="frwC"
                     /locus_tag="ACHJ1G_002204"
     CDS             4643..5722
                     /gene="frwC"
                     /locus_tag="ACHJ1G_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4764053.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5737..6057
                     /gene="frwB"
                     /locus_tag="ACHJ1G_002205"
     CDS             5737..6057
                     /gene="frwB"
                     /locus_tag="ACHJ1G_002205"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4764054.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6108..8405
                     /gene="pflD"
                     /locus_tag="ACHJ1G_002206"
     CDS             6108..8405
                     /gene="pflD"
                     /locus_tag="ACHJ1G_002206"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4764055.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8371..9249
                     /gene="pflC"
                     /locus_tag="ACHJ1G_002207"
     CDS             8371..9249
                     /gene="pflC"
                     /locus_tag="ACHJ1G_002207"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO4764056.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9251..9592
                     /gene="frwD"
                     /locus_tag="ACHJ1G_002208"
     CDS             9251..9592
                     /gene="frwD"
                     /locus_tag="ACHJ1G_002208"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4764057.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9579..10430)
                     /gene="yijO"
                     /locus_tag="ACHJ1G_002209"
     CDS             complement(9579..10430)
                     /gene="yijO"
                     /locus_tag="ACHJ1G_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4764058.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10579..12312)
                     /gene="eptC"
                     /locus_tag="ACHJ1G_002210"
     CDS             complement(10579..12312)
                     /gene="eptC"
                     /locus_tag="ACHJ1G_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4764059.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12495..15146)
                     /gene="ppc"
                     /locus_tag="ACHJ1G_002211"
     CDS             complement(12495..15146)
                     /gene="ppc"
                     /locus_tag="ACHJ1G_002211"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4764060.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15498..16649)
                     /gene="argE"
                     /locus_tag="ACHJ1G_002212"
     CDS             complement(15498..16649)
                     /gene="argE"
                     /locus_tag="ACHJ1G_002212"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4764061.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16803..17807
                     /gene="argC"
                     /locus_tag="ACHJ1G_002213"
     CDS             16803..17807
                     /gene="argC"
                     /locus_tag="ACHJ1G_002213"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4764062.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17815..18591
                     /gene="argB"
                     /locus_tag="ACHJ1G_002214"
     CDS             17815..18591
                     /gene="argB"
                     /locus_tag="ACHJ1G_002214"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4764063.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18652..20025
                     /gene="argH"
                     /locus_tag="ACHJ1G_002215"
     CDS             18652..20025
                     /gene="argH"
                     /locus_tag="ACHJ1G_002215"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4764064.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20292..21209
                     /gene="oxyR"
                     /locus_tag="ACHJ1G_002216"
     CDS             20292..21209
                     /gene="oxyR"
                     /locus_tag="ACHJ1G_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4764065.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVEQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21192..22592)
                     /gene="sthA"
                     /locus_tag="ACHJ1G_002217"
     CDS             complement(21192..22592)
                     /gene="sthA"
                     /locus_tag="ACHJ1G_002217"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4764066.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22923..23573
                     /gene="fabR"
                     /locus_tag="ACHJ1G_002218"
     CDS             22923..23573
                     /gene="fabR"
                     /locus_tag="ACHJ1G_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4764067.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23573..23932
                     /gene="yijD"
                     /locus_tag="ACHJ1G_002219"
     CDS             23573..23932
                     /gene="yijD"
                     /locus_tag="ACHJ1G_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4764068.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23972..25072)
                     /gene="trmA"
                     /locus_tag="ACHJ1G_002220"
     CDS             complement(23972..25072)
                     /gene="trmA"
                     /locus_tag="ACHJ1G_002220"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4764069.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25186..25376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25441..27285
                     /gene="btuB"
                     /locus_tag="ACHJ1G_002221"
     CDS             25441..27285
                     /gene="btuB"
                     /locus_tag="ACHJ1G_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4764070.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27230..28087
                     /gene="murI"
                     /locus_tag="ACHJ1G_002222"
     CDS             27230..28087
                     /gene="murI"
                     /locus_tag="ACHJ1G_002222"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4764071.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6467 a         7118 c         7804 g         6912 t
ORIGIN      
        1 attttgcgtt caaactcccg gacaagccgg gagtttggag taggttattc ccactcttgc
       61 aggaaacgct gaccgtactg gtcggctacc agcagagcgg cgtaaacctg atctggcgtc
      121 gcgccgccag gcatgttgtg gatggtttca ccttctgcac atgccgcttc tgccacaatt
      181 cgcattttcg ccgggacatc ttctttaata tccagttgag cgagagttat tggcaaacct
      241 accgcatggc taagtgcagc tacggtttcg atttcctcaa ccggcgcgtt ttccagtacc
      301 agctgcgtca gcgtaccgaa tgccactttt tcaccgtgat aatagtgatg cgcgtccggg
      361 atagcggtca ggccgttatg caccgcatga gccgccgcca gtccgccgct ttcaaaacca
      421 acgccgctta aataggtgtt cgcttcaatc acgcgttcca gcgcaggggt tactacatgc
      481 tgttcggcgg caagcatcgc tttttcgcct tcttccagca gagtgttgta gcacagttct
      541 gccagtgcca gcgccgcctg ggtgcacttg ccgcccgcca tagtggtagc gccgctacgg
      601 gaacaggcgc gtgcttcaaa ccaggttgcc agcgcatcgc cgatacccgc cgctaacaga
      661 cgtgcaggag cgcccgcgac gattttggtg tcgacaatga ccatattcgg gttatttggc
      721 aacagtagat agcggtcaaa ctcaccctca tcggtgtaga taacagacaa tgcgctgcac
      781 ggtgcatcgg tagaggcgat agtcggtgcg atcgctaccg gaacacccat gaaatgtgcc
      841 agtgctttgg cagtatcgag ggtttttccg ccaccgatac cgagaattgc gccacactgc
      901 gcagtctccg cgatgccacg cagacgatcg atctcatttt gcgaacattc accgccaaac
      961 ggcgcaattt ctactaccag tccagcatct ttaaagcttt tctcgacagt ggattgagca
     1021 aaacctaaaa caaatttgtc acccaccact aaccagcgtt ctgccagcgg cttcaggtat
     1081 tcgcccagac gattaatcac atcagcgccc tggatgtatt tacccggtga ttgaataatg
     1141 cggtccataa ttgctccttt agagatgagt agtgccaaat gcggcattcc agtcgtgttc
     1201 gaacttctct atagctgact ctaccgcagg ggtgttgagc atttgttgcg ctacatctaa
     1261 aggcagggtg atggactcac atcctgccag caaacaatcc agtgcctgac gcggcgtttt
     1321 aaagctggct gcaagcacca tgctttctgg cgcgtgcatt tctaacagcg cttgcagctc
     1381 ctgaaccgta cgaatgccgt ccccgccctg ggcatctacg cggttaacat acggagcaac
     1441 gtattttgcc cctgccagcg cggccagtaa cccttgtgca gcactgtaca ctgcggtgcc
     1501 aagcgtagta ataccctctt ttttcagcat cttaatggcg gccagacctt cggaagtcac
     1561 cgggattttc accacaatgc ccggaatggc gtcgcgcagg cgcttcgctt cttccaccat
     1621 accctgcgcg tcacgactca tagtctgagc aaacagaatg ccctcatcac caatcgcttt
     1681 ttgcagacgc ggcagaactt cccatatgga ctccttgctg gcagcgataa tgctcgggtt
     1741 agttgtcacc ccggcaatgg ggaatatgcg tgccagacgt tcgacttctg cgacgttagc
     1801 ggtgtccaga tacagttcca tgatgtttcc ttttacagtt ccagttcatg ttgcagcagg
     1861 ctggcgatag cgtcggcaga ggcggcgtta accagcgcgt tacggaattc ttcgtgcatg
     1921 atgcggcgag cgaggcgcga gaaaatgcgc atgtgctgat cgcccgcagc gtgtttgttc
     1981 agggttaaca taatgatgaa ttgcgcttca tcatcgcccc agcgcaccgg cgcttgcaga
     2041 cgcgctacac tgatggtgga ttgctcaatg tgttctgatt tgctgtgggg aatggcaaaa
     2101 ctaaagccca gaccggtaga gaaaacggcc tcgcgcgccc acaagtcggc ttccagttta
     2161 cgcggatagc ggcagcggcc cgccagcagc aggttatcgg tcatgccttt gagcacttct
     2221 tctttgctgc gtcagtcgct ttccagcgtg atgcactcgg cggtgaccag cggtgcgtcc
     2281 tgttgggtca tgcggaattg cgccagcagg tgttccactt ccagcgaagt acggcaggcc
     2341 attgcctggt tgagcaactg gcggcattcg cggctatcaa gttgcgccat tcgcgctttc
     2401 gccgccggaa ttgacggtgc gctcatgctc agctcatcca gccctaaacc gaccagcaac
     2461 ggcagcacgg aacctttcgc tcccagctca ccgcacagac caatccattt gccctggcga
     2521 tgcaccgctt gcacggcgta atcgagcgcc cgcaagaatg ccggattcag gctgttgtag
     2581 tgacgagtaa ccttagcatt atcgcgatcc accgccagca gatattgcgt caggtcatta
     2641 ctaccaatac taaagaaatc aatctcttcg cagcattgat cgatgatgaa catcaccgac
     2701 ggcacttcca gcataatgcc gagctggatc ttctcatcaa acggaatgtg ttcgttacgc
     2761 agttgctgtt tggcttccgc cagtttttct ttcacccata agatctcttc cattgaagag
     2821 atcatcggga tcatgatttt caggctgccg tgagcggagg cgcgcaggat cgaccgtagt
     2881 tgtgtggtga acagcgaggc gtattcttca taaatacgca cagcgcgata accgaggaac
     2941 gggttagctt ctgccggaat gttcagataa tcaacgggtt tgtcgccgcc aatgtccata
     3001 gtgcgcacaa taatgctgcg tccgttggca gactccagcg cctgacaaaa aatgttgtac
     3061 agctcgcttt cgcccggtgc actggtgcga tccatataga gcatttcagt gcggaacaaa
     3121 ccaacgcctt ccgcaccatt gccgaatgcg gcctgcgctt ccacggagtg agcgatgtta
     3181 gcggcaattt caatgcggat accgtcagcg gtacgggctt gttgggtcag ccagacacgc
     3241 tgttgctcac gcagggcatc ctgtacgcgg gcttcttgct gataataacg ggctacggct
     3301 tcccctggct caaccacaat cgctccggcg ttgccgtcga tataaatcgt ttgatgttgc
     3361 cacggggtaa gggcatcaat atccacacca accagcgtcg gaatgttgaa cgaacgggca
     3421 aggatcaccg tatgtgaggt ggtgccgccg cttttgagca acaatccttt gaggtgattt
     3481 ttatccagtt cgaggaactg gctgggggtc agttcatcag ccatacaaat ggcgggctgc
     3541 gtcagtttgc ccggtgccgg gaagcgttgc tcaccgtaga tttgctggag taactggaag
     3601 cagacgtcgc gtacgtccag ggcacgttct tgcaggtagc tgctgctgga acgggaaaac
     3661 tcttcacaaa agtgattcgc actggcgaca atcgcttcgg cgcagcttaa tccggcgctg
     3721 acgcctgcca gtaaatgttc gtgcagggaa gtatcgccag ccagggatcg gtgcgcttcc
     3781 agaatcgcgc tggtggcacc gtcgctatcc agcagacgaa actcaatgtt tttcagtacc
     3841 agcgtcaggc cgttttccag tgcggattgc tcggcgtcaa cgtctttggc tgcgggaaga
     3901 ttactcagcg cattgagatc taaagaagag atcggcgtca gaatgccgcc tgcgctaccg
     3961 ctgcacacgg tgcgggcgcg gataatttgc ggattcagat tggtcagtga aaccggcagt
     4021 ggttccagtt cgtcagattt aacttccgcc agcggtgcgt cgcagtgggg gaattcatcg
     4081 cgcagccatt ggcttaaacg ctggtgcgct tcctgttcgt cggccccgga aatcagtaac
     4141 cggtagttat cgcccgccag cgtgtcggtg ccaatcagcg caagggcgct tttggcgttg
     4201 cccttgcggt cagtgcgcag gttatgccac tcaatttgtg atgaaaaagt attacacagc
     4261 gtttcaacgt ggcttgccgg acgtgcatgt acgccgttag gtagctcaca aataaattcc
     4321 acaatcaggg ccattgcctc tctcccggta cggttttctg tttacaggat aagagcgcac
     4381 ggcaacggcc tgccatgtga caaatctgcc aaaagctgga caaatgtaat gtaaccgtca
     4441 atttgcgacg cgtctcacaa gacgccgttt tgcggcatgc ttccggatta tcgcaagtta
     4501 tgaggcggat cgcatttttg tactgatatt acaaaagtcc agtaaatggc ctttttatcc
     4561 actgtttgct ccgcctgcga ttgcctattg tttcctccat caaaaatatg gacatgggct
     4621 ttcgcaagcc cgggagcagc ttatgaatga gttggtgcag atcctgaaaa atacccgtca
     4681 gcatttaatg acgggcgttt cacacatgat tcccttcgtg gtatcgggcg gtattttgct
     4741 ggcggtttcc gtcatgttgt atggcaaagg cgcagtgccg gatgccgtag ccgatccgaa
     4801 tctgaaaaaa ctgtttgata tcggagttgc aggtttgacg ctgatggtgc ctttcctcgc
     4861 cgcttacatc ggttactcca ttgcagagcg ttctgcgctg gccccgtgcg ctatcggtgc
     4921 ctgggttggt aacagctttg gtgcgggctt ctttggtgcg ctgatcgccg ggattatcgg
     4981 cggcatcgtg gtgcattacc tgaagaaaat tccggtgcat aaagttctgc gctcggtgat
     5041 gcctatcttc attattccta tcgtcggcac actgattacc gcaggcatca tgatgtgggg
     5101 cttgggcgag cctgtagggg cgttgaccaa cagcctgact cagtggcttc aggggatgca
     5161 gcagggcagc attgttatgc tggcggtgat catgggtctg atgctggcgt tcgatatggg
     5221 cggtccggtt aacaaagtgg cctatgcctt catgctgatt tgcgttgctc agggtgttta
     5281 taccgtggtg gccattgctg ccgtgggtat ttgtatcccg ccgctgggga tggggctggc
     5341 gacgctgatt ggtcgtaaaa atttctccgc agaagagcgc gaaaccggca aagcggcgct
     5401 ggtgatgggg tgcgttgggg ttactgaagg ggcgattcct ttcgccgctg ccgatccgct
     5461 gcgtgtcatt ccttccatca tggtcggttc tgtttgtggt gcggtaactg cggcgctggt
     5521 cggtgcgcag tgctatgcag gctggggtgg tctgattgtg ctgccggtgg ttgaaggcaa
     5581 gctgggttat atcgcagcag tggctgtcgg agcagtggtg acggctgttt gcgtgaacgt
     5641 gctgaaaagt ctggcgcgta aaaatgggtc ttcgactgat gagaaggaag acgacctgga
     5701 tttggatttt gaaattaatt aattgaggaa cgagccatga cgaaaattat tgcagtaacc
     5761 gcgtgtccat ctggtgttgc ccatacctat atggcggcag aggcgctgga aagcgccgcg
     5821 aaagcgaaag gctgggaagt gaaagtagaa actcaggggt caattggtct ggaaaacgaa
     5881 ctgactgcgg aagacgtggc gagcgccgat atggtgattc tgaccaaaga tatcggcatc
     5941 aagtttgaag agcgttttgc tggcaagacc atcgtgcgcg tcaacatcag cgatgcggta
     6001 aaacgtgctg acgccattat gagcaaaatt gaagctcacc tggcgcaaac cgcttaagtt
     6061 cttctcccgc tcgcaagggc gggttcgctt tcccacagga gttcctcatg acgaatcgta
     6121 tctctcgcct caaaactgca ctgttttcca atacccgtga aatctcgctg gagcgggcgc
     6181 tgctttatac cgccagccat cggcaaaccg aaggcgaacc ggtgatattg cgccgggcga
     6241 aagcaacagc gtatatcctt gaacatgttg aaatttcgat tcgtgatgaa gaactgattg
     6301 ccggtaaccg caccgtaaaa ccgcgcgccg ggattatgtc gccggaaatg gacccttact
     6361 ggctgctgaa agagctggat caattcccga cgcgtccgca ggaccgcttt gctatcagcg
     6421 aagaagataa acgtatctac cgcgaagagt tgttcccgta ctgggaaaaa cgttcgatga
     6481 aagatttcat caacgggcag atgacagatg aagtaaaagc cgcgaccagc acgcagattt
     6541 tcagcattaa ccagacggat aaaggccagg ggcacattat tattgattac ccacgcctgc
     6601 tgaatcacgg gctgggggag ctggtggcgc agatgcagca acattgtcag caacagccgg
     6661 agaatcactt ttatcaggcc gcgctgttac tgctggaagc ctcgcagaaa cacattctgc
     6721 gttacgctga actggcggaa acaatggcgg caaactgcac agatgcccag cgtcgcgaag
     6781 aactgctgac tattgcggag atctcccgcc ataacgccga acataagccg cagacgttct
     6841 ggcaggcgtg ccagttattc tggtacatga acatcattct gcaatacgaa tccaacgcca
     6901 gttcgctgtc tttagggcgc ttcgaccagt atatgttgcc gttctatcag gcttctttaa
     6961 cccagggcga agatccggcg ttcctgaaag aactgctcga atctttatgg gtgaaatgca
     7021 acgacatcgt gctgttgcgc tccaccagca gcgcgcgtta tttcgcaggt ttcccgaccg
     7081 gctataccgc actgctcggc gggttaaccg agaacggacg tagcgcggtg aacgtgcttt
     7141 cgttcctttg ccttgacgcc tatcaaagcg tgcaattacc gcaaccgaac ctcggcgtgc
     7201 gcactaacgc cttgatcgac acgccgttcc tgatgaaaac cgccgaaacc attcgcctcg
     7261 gcaccggtat tccgcaaatc tttaacgatg aagtggtggt accagcgttc ctcaaccgtg
     7321 gcgtttcgct ggaagatgcg cgcgactatt ccgtagtggg ctgtgtggaa ttatctattc
     7381 ccggcagaac ctacggcttg catgacatcg cgatgtttaa tctgctgaaa gtgatggaaa
     7441 tctgcctgca tgaaaatgaa ggcaatgctg cgctgactta tgaaggttta ctggagcaga
     7501 tccgcgccaa gatcagccac tacatcaccc tgatggttga gggcagcaat atttgtgata
     7561 tcggccatcg cgactgggca cctgtaccgc tgctctcgtc ttttatcagc gattgtctgg
     7621 aaaaaggtcg cgatattacc gatggcggcg cgcgttataa cttctccggc gtacagggga
     7681 tcggtatcgc caacctgagc gattctctcc atgcgttgaa agggatggtt tttgagcaac
     7741 agcgtttaag ttttgacgaa ttgctgtcgg tattaaaagc caactttgca acgccagaag
     7801 gcgaaaaagt ccgtgctcgc ttaattaacc gcttcgagaa atacggtaac gatatcgacg
     7861 aggtggataa catcagcgcc gaactgttac gccactactg caaagaagtg gaaaaatacc
     7921 agaacccgcg cggtggttac ttcacgccgg gatcgtatac cgtttctgct cacgtcccgt
     7981 tgggatcggt ggttggcgcg acgccagacg gtcgttttgc cggggaacaa ctggcagacg
     8041 gcggcttgtc acctatgctg ggccaggacg cacaagggcc aacagcggta ctgaagtcag
     8101 tcagtaagct cgataatacg ctgctgtcta acggtacgtt gctgaacgtg aaattcactc
     8161 cggcgaccct ggaaggtgaa gcgggattac gcaaactggc cgacttctta cgggcgttta
     8221 cccagcttaa gttgcagcat attcagttta acgtggtgaa cgccgacacg ttgcgggaag
     8281 cgcaacagcg cccacaagat tatgccgggc tggtggtgcg cgttgccgga tacagcgcct
     8341 tctttgtcga actgtcgaag gagatccagg atgacatcat ccgccggaca gcgcatcagc
     8401 tgtaacgttg tggaaacgcg ccgcaatgat gtggcgcgca ttttcaacat tcagcgttat
     8461 tcactgaatg acggcgaggg cattcgtacg gtggtctttt ttaaaggctg tccgcatctt
     8521 tgcccatggt gtgctaatcc ggagtcgatc tccggcaaaa tccagacggt acgcagagag
     8581 gcgaaatgtc tgcactgtgc gaaatgtttg cgtgatgcgg atgaatgccc ctccggggcg
     8641 tttgaacgga ttggtcgcga tatcagcctt gacgcgctgg aacgggaagt gatgaaagat
     8701 gacattttct ttcgcacgtc cggcggcggc gtcacgcttt ctggcggcga agtgttaatg
     8761 caggcggagt ttgctacccg ttttttacag cggctgcggt tgtggggcgt gtcatgcgcc
     8821 attgaaactg ccggagacgt gccagccagc aagctgttac cactggcgaa attgtgcgat
     8881 gaagtgctgt tcgatttaaa aattatggac gcgactcagg cgcgggatgt ggtgaagatg
     8941 aacctgccac gcgtgctgga gaatctgcgt ttgctggtga gtgagggtgt caacgtgatc
     9001 ccgcgtttac cgttgatccc tggtttcacg ctcagccggg agaatatgca gcaggcgctg
     9061 gatgtactga tcccgctgaa aatcaggcag atccatctgt taccgtttca tcagtacggc
     9121 gaaccgaaat accgcctgct ggggaaaaca tggtcgatga aagaggtgcc tgcgccgtcg
     9181 tcagccgatg tgacaacgat gcgcgaaatg gcagaacggg ccggatttca ggttaccgtg
     9241 ggaggttaaa atggcatacc tggtggcagt aaccgcctgc gtaagcggcg tggcgcatac
     9301 ttatatggcg gcggaacggc tggaaaagtt gtgccagtta gagaagtggg gcgttagcat
     9361 tgaaactcag ggcgcgctgg gaacggagaa tcgtttagcg gacgaggata tccgtcgggc
     9421 ggatgttgct ctgttgatta cggatatcga gcttgccggt gccgagcgat ttgaacattg
     9481 ccgctatgtg caatgcagca tctacgcatt cctgcgtgag ccgcagcggg taatgagcgc
     9541 ggtgcgcaaa gtgctttctg cgccgcagca aacccatctt attctggagt agtcggtttt
     9601 tctgtcagtt ggctgtggta ctgccgacga tattccgacg gcgagcgttc ggtgttttta
     9661 cgaaacagac ggcagaagta gttgctgtcg acaaagccgc aggcgtgcgc cacttctttt
     9721 accttcaggt catagccttt caataacgtc ttagcgtgct ccagtcgcgt gtgattcagg
     9781 tattcgttaa aaccaatggc ccccgttttt tgaaacaggt gcgagagata gtttggtgaa
     9841 atatagaatg cctgtgcgac agattcgcgg gtaagcgcgg aggcatagcg ttcgtcgata
     9901 taatcgcgaa tagcttcaaa tagtgcctgg ctgcgtgagg cggtctggat ttggctgccg
     9961 agcaaatcgc ggcagtggct aagcaggctg gcgacgataa gccttgccgt ttgctgctcc
    10021 tgcggctgca tttgcatttc attgagcgtt tgtagcagaa aagaacctat gcgtgggccg
    10081 cgccgggcga cgtgttgctt cgccagattt tgatattgtt tgccatccca ttgcacgacg
    10141 ctgaagccga gttgctgttt gccaaacagc acgctaaagg tagtagcggg ggcttgccat
    10201 tgtggaaaat tccagccacc agcaggaacg tacagcacat cgccgggaac taacgttgct
    10261 ccagcgccgg tatcaacaaa ctcaccttcc agcacaattt ccagccgtgg aaaatcgacc
    10321 tgatacgcga gatcgggcac cgggccgtta ctgctggcaa agtaaatctg gcgcagggac
    10381 agcgggccgt tgatcaggcg ggagagcaga tagctgacgt cgtgatacat gttattccct
    10441 taggtatgta gacgggaaaa cagtaactgg cattattaaa aaaggccacc tgaaaagtgt
    10501 ggcctgaggg gagttcgatg taaacattgc ggcagtatcg catccggcaa atagcgccag
    10561 acgcgataca aaggtcattt actgattacc cacctgatcg ccatacggca gcgtgtcgta
    10621 atcgatcagc gcgtttttct tgtacgggtt accaatccag cgggtagttt ctttgaactg
    10681 cggattcacc actgaacggg ttggatcgta accgtcgtaa gataagcctg ctaaatctga
    10741 ccaggtgtgg atcagttccg ccaggctgta tttacgatca acatcctgcg agaaatcacg
    10801 cggatgagtc gcttgccatt tttccgacgt ccacagcagg aacggaatgg tgtacatgtg
    10861 gcgcgtcggg ttgtcttcgt tacgcccctg agttttgtgc ggcggcgtgt cgtaaacctc
    10921 ttcaccgtgg tcagagaaat aaaccaggaa accgttcggg tttgctgctt taaagtcttt
    10981 aatcaggctg gcaaccacat gatcgttata caggttagcg ttgtcataat cgttatatga
    11041 ctccagctct tccgcgttta atccaggcgg aacatgatcg gtattgccat caaacttgcc
    11101 ctgattttcc gggtagcggt atttgtattt gatatgcgta cccagcagat gaacgatgat
    11161 cagtttcttc ggcgccgggt ccttcagcac ttcctggaat ggtttcagca cgttggtgtc
    11221 gtattcacgc gcactctgcg tacgttgctg gttcatatag aactgtttgt cggtctgctt
    11281 cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa
    11341 ggttttataa cctgcctgtt tcatcatgtt catcagcgac ggttgtgtca ggtacaggtc
    11401 cgggttcttt tcattagcaa aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg
    11461 agacgtaacc acgttattaa acacggtcag attcggatcg gttttatgca gcgcatccag
    11521 ctccggcgtg gtttcacgcg gataaccgta cagactcatg cgcccgcgtt gggtcgattc
    11581 gccaatcacc agcactaagg tacgcggttc gttacccgat tcatctttga aattagccag
    11641 aggcggcaag gcattatttt cattcagtaa ctttgttagc gagtttagtt gctgacgata
    11701 ctgataatag ccggtcagga attgccacgg tgcggcaggc tccatacgcg aggccaggtt
    11761 atccaacgtt ttctcaaacg gcttgttttt gataaacgta ttcatggcga tcggatgcag
    11821 aatcaagcca taaagcaggg caaaagagac aacataacgc cacggctttg gaatatagac
    11881 cgggtgcagg cgtgtccaca gcagaactgc caccgccgta taggccagcg cgataagcac
    11941 aattttcagg ctgaaatact ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa
    12001 catcacaaac agaacgctct gcgagaactc ctgcccgtag atgacgtagt agcatagcgc
    12061 cgccagagag gccgcccata gcaccacgcc gattactgcg gcaataattt taatccgctt
    12121 cggaaagagg aataccggga tcaaccacag cgagctgaat aacagcgagt cgcgaatgcc
    12181 gttggtgcca ctataaccac tgatgtaaat aatggcctgt agcagagtag agaaaaacca
    12241 aaagtagagc agtgcccaac ccagggcttt ccagctaaaa aggggtttag cctggacttc
    12301 tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc gccgaatgta acgacaattc
    12361 cttaaggata tctgaaggta tattcagaat ttgaataaaa tgcagacaga aatatattga
    12421 aaacgagggt gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt
    12481 gcagaagagg aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc
    12541 attaacgctt gttcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag
    12601 cggtgcagta actcggcctg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga
    12661 atagactctg caatccacgg cagatcggcc attagatggg aatcgttggc aatcgccagc
    12721 accactttga tgtcttcttc ttgcaggttg cgtaactctt tacctaacgg ccacagtgct
    12781 ttgtctacca ggcgttggtc atagtattcc gccagccaca ggtctgcttt ggcgaagacc
    12841 atctccagca tgccgagacg cgtcgagaag aatggccaat cgcggcacat ggcttccagc
    12901 tcgctctgtt tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg
    12961 gggagcatca ggcggttttg cgtccaggca aaaatccacg gtatggcgcg cagcgactcg
    13021 acgccgccgg ttgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccaattct
    13081 tgttccggcg tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca
    13141 cggtagagat cgcaggagat gactgacagt tcatccataa tgcgacgcca gctctctttc
    13201 ggctcaggtg gtggcagcag gttggcttcc agaatagccc cggtgtacag cgacaggctg
    13261 ctgacggtga tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg
    13321 cgcaggccac ctttcaggct tcctggcggt tgtgacagca gcgccgcatg agcaggtgcg
    13381 ccgccgcgac caatggaacc gccgcgaccg tggaacaacg tcagctcaat acccgctttt
    13441 tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg cccaggaagc tgccatcact
    13501 cccgcatctt ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg
    13561 ccacgatacc agtcaatatt gagcagctgg gtcatgacat cgttggcgtt gttcagatca
    13621 tcgagggttt caaacagcgg agcaaccggc atcgcaaacc cgatacccgc ttctttcagc
    13681 agcaggtgga cagccagtac gtcggacggc gttttcgcca tcgagatcac gtaggcggca
    13741 atggagcctt gcggtgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg
    13801 gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg agttcagttc gcggatcagg
    13861 aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc
    13921 agctcgccca gcgcttcggt atgacgcgtg ctctcctgac ggatatcaat acggaccagc
    13981 ggtacgccga aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata
    14041 cccatgccac acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg
    14101 ttttgtgtca gcaggccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc
    14161 catgcctgtg tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct
    14221 gcggcacctt cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt
    14281 tcagaaacca gcacctgaat atctttcagg aacaaatcgg tggctttcca gcggctgagt
    14341 agcaggacgt ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcgccgccc
    14401 atccacgaag taaaacggac cggaacaaat tcgacgggca gtttgtagcc gaggttctct
    14461 tccagttgtt cgttcagttc gcgcaggtaa tttggtacgc cttgccacag gctgttttcc
    14521 actacggcaa agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca
    14581 tcggtatgcc atgactgggc gattaactgg cgcaggcggc gcatcagctg gttgtgttcg
    14641 tagtcagcga tatctttgtt atcgagctgt tttaaacagg cgttcacttc caccattttg
    14701 tggatcagtg tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac
    14761 agcgattcca ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagttta
    14821 cgcagggtgc gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg
    14881 tggtattgct cggcggtgtt ggccaggttc aggaactgac taaacgcacg cgcaacgggc
    14941 agcagctcgt cgttcgacaa attttgtaag gtggtgagca actcctggcg gttagcatca
    15001 ttgccagcgc gtgaagattt cgacaactta cggatagttt ctacgcgttc aagaatgtgt
    15061 tctcccaacg catccttgat ggtttctccc agcactttgc cgagcatact gacattacta
    15121 cgcaatgcgg aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag
    15181 ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt
    15241 ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat
    15301 aaatagcagg aatttacgcc attaaattca cgacgcttta aataagcgta acttatggaa
    15361 atgttaaaaa atcgccatac ataacaccaa aggtgtaggt cggataagat gcgcaagcat
    15421 cgcatccgac attattgcgg cacaggagtt tggcaacggt gccggatgcg gcgcgagcgc
    15481 cttatccggc ctacgtttta atgccagcaa aaatggtgaa ttacctgggt tatcagttcg
    15541 cgggtgggct tgataaaccg tgtttccaga tattcatcag gttgatgagc ctgattaatt
    15601 gagccaggcc ccaacaccag cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag
    15661 tagttcacca cttcggtctt tgctccgagc aatttctcaa ctacttcaac cagttgatga
    15721 ttcggtgggc attcatagcc agggatcggc ggatgcagct cgtcgaccgt cagacgaccc
    15781 ggccagcgtt cgctcaccgg agccaatgca tcgttgagca aaccattaag ttcattgagt
    15841 gtcatgccag gcagcggacg aatatccata tgcaactcac agcaagcgca aatacggtta
    15901 gaagcgtcgc caccgtgaat atgcccgagg ttgagcgtag ggtatggcac ggtaaacgct
    15961 tcgtagtgat aacgttcttt caggttatcg cgcaattgca aaatatgccc gatggcgtcg
    16021 tgcattagtt cgatagcgtt aactccgcgt gctggatcgc tggagtgccc cgactggccc
    16081 tgaatacgga tggcgttaga gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc
    16141 ggttcgccaa tgatggcgca atccgggcgc agggcggtag tttcggcaaa ataacgcgct
    16201 ccggccatac tggtttcttc atcagcagtc gccagaatgt agagcggttt tttcagtttc
    16261 gtgacgtcga catcgcgtag cgcatcaagg ataaacgcaa aaaagccttt catgtcggcg
    16321 gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag
    16381 cgaccgtcat caaatggcac cgtatcggta tgccccgcca gcaacaagcc gccagccccc
    16441 tgtccgatac ttgccagcat attgaatttg ttgcgagttc ctggcacagg ctgcacttcc
    16501 acattgaagc ctaaatcttt aaaccagtcc gccagcagag tgattaaatc tgcattgctt
    16561 tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc acggtaaatc
    16621 tcgataaatg gcggtaattt gtttttcatt gttgacacac ctctggtcat gatagtatca
    16681 atattcatgc agtatttatg aataaaaata cactaacgtt gagcgtaata aaacccacca
    16741 gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga cataagaagg tgaatagccc
    16801 cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc tggcgcagag ctagtgacct
    16861 atgtaaatcg ccatccgcat atgaacataa ccgctttgac tgtttcagcg caaagcaatg
    16921 atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt gatctgccgt
    16981 tgcagccgat gtcggatatc agcgagttta gcccaggggt ggacgtagtg tttctcgcca
    17041 ccgcccatga agttagccac gatttagcgc cgcagtttct tgaagcgggc tgcgtggtgt
    17101 tcgacctttc cggcgcgttt cgtgttaacg acgccacctt ctatgaaaaa tattacggct
    17161 ttacccatca atacccggaa ctgttggaac aggcagccta cggtctggcg gagtggtgcg
    17221 gtaataaatt aaaagaagcg aatttgattg cggtgccggg ctgttatccg acggcggcac
    17281 agctggcgct gaaaccgttg attgatgccg atcttcttga cctcaatcag tggccggtga
    17341 tcaacgccac cagcggcgtg agcggtgcag gacgtaaggc agcgatttca aatagttttt
    17401 gtgaagttag cctgcaaccg tatggcgtct ttacccatcg ccatcaacca gagatcgcca
    17461 cacacctcgg tgctgacgtt atcttcaccc cgcatctggg caatttcccg cgcggcattc
    17521 ttgaaaccat tacctgccgc ctgaaaccgg gtgtgaccca ggcgcaagtg gcgcaagcgt
    17581 tacagcaggc gtatgcccat aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc
    17641 tgaaaaatgt cgttgggctg ccatgttgcg atatcgggtt tgccgttcag ggcgagcatc
    17701 tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt
    17761 gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg
    17821 aatccattaa ttatcaaact gggcggcgta ctgctggata gtgaagaggc gctggaacgt
    17881 ctgtttagcg cactggtgaa ttatcgtgag tcacatcagc gtccgctggt gattgtgcac
    17941 ggcggcggtt gcgtggtgga tgagctgatg aaagggctga atctgccggt gaaaaagaaa
    18001 aatggcctgc gggtgacgcc tgcggatcag atagacatta tcaccggagc actggcagga
    18061 acggcaaata aaaccctgct ggcatgggcg aagaaacatc agattgcggc cgtaggtttg
    18121 tttctcggtg acggcgacag cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt
    18181 ggactggcgc agccaggttc gcctaagctt atcaacacct tgctggagaa cggctatctg
    18241 ccggtggtca gctccattgg cgtaacagac gaagggcaac tgatgaacgt caatgccgac
    18301 caggcggcga cggcgctggc ggcaacgctg ggcgcggatc tgattttgct ctctgatgtc
    18361 agcggcattc tcgacggcaa agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa
    18421 caactgattg agcagggcat tattactgac ggcatgatag tgaaagtgaa cgcggcgctg
    18481 gatgctgcgc gcacgttggg ccgtccggta gatatcgcct cctggcgtca tgcggaacag
    18541 cttccggcac tgtttaacgg tatgccgatg ggtacgcgga ttttagctta agttttgttg
    18601 gccggaggcg cagctttccg gcattgaatt tcaaaataag gaaacagagt tatggcactt
    18661 tggggcgggc gttttaccca ggcagcagat caacggttca aacaattcaa cgactcactg
    18721 cgctttgatt accgtctggc ggagcaggat attgttggct ctgtggcctg gtccaaagcc
    18781 ctggtaacgg tcggcgtgtt aaccgcagaa gagcaggcgc aactggaaga ggcgctgaac
    18841 gtgttgctgg aagatgttcg cgccaggcca caacaaatcc ttgaaagcga cgccgaagat
    18901 atccatagct gggtggaagg caaactgatc gacaaagtgg gccagttagg caaaaagctg
    18961 cataccgggc gtagccgtaa tgatcaggta gcgacagacc tgaaactgtg gtgcaaagat
    19021 accgttggcg agttgctgac ggctaaccgg cagctgcaat cggcgctggt ggaaaccgca
    19081 caaaacaatc aggacgcggt gatgccaggt tacactcacc tgcaacgcgc ccagccggtg
    19141 acgttcgcgc actggtgcct ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg
    19201 caggatgcgc ttaagcgtct ggatgtcagc ccattaggct gtggcgcgct ggcgggaacg
    19261 gcctatgaaa tcgaccgtga acagttagca ggctggctgg gctttgcttc ggcgacccgt
    19321 aacagtctcg acagcgtttc tgaccgtgac catgtgttgg aactgctttc tgctgccgct
    19381 atcggcatgg tgcatctgtc gcgttttgct gaagatctga ttttctttaa caccggcgaa
    19441 gcggggtttg tggagctttc tgaccgcgtg acttccggtt catcattaat gccgcagaag
    19501 aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg gtcgggtgca gggggcgtta
    19561 accggcatga tgatgacgct gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa
    19621 gacaaagaag gtctgttcga cgcgctcgat acctggctgg actgcctgca tatggcggcg
    19681 ctggtgctgg acggcattca ggtgaaacgt ccacgttgcc aggaagcggc tcagcagggt
    19741 tacgccaacg ccaccgaact ggcggattat ctggtggcga aaggcgtacc gttccgcgag
    19801 gcgcaccata ttgttggtga agcggtggtg gaagccattc gtcagggcaa accgctggaa
    19861 gatctgccgc tcgacgagtt gcagaaattc agcccggtga ttgacgaaga tgtctatccg
    19921 attctgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt ctcaccgcag
    19981 caggtggcgc aggcaattgc ttttgcgcag gcgcggttgg ggtaagaaca tttatatgta
    20041 taaatttgag cctggcttat cgccgggctt ttttatggca aaaaaaagcg gatcctggag
    20101 atccgcaaaa gttcacgttg gctttagtta ttcgagttga gaaactctcg aaacgggcag
    20161 tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc agtcagaatg
    20221 cttgataggg ataatcgttc attgctattc tacctatcgc catgaactat cgtggcaatg
    20281 gaggatggat aatgaatatt cgtgatcttg agtacctggt ggcattggcc gaacaccgcc
    20341 attttcggcg tgcggcagat tcctgccacg ttagccagcc gacgcttagc gggcaaattc
    20401 gtaagctgga agatgagttg ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt
    20461 tcacccaggc gggaatgctg ctggtagagc aggcgcgtac cgtactgcgt gaggtgaaag
    20521 tccttaaaga gatggcaagc cagcagggcg agacgatgtc cgggccgctg cacattggtt
    20581 tgattcccac agttggaccg tacctgctac cgcatattat ccctatgctg caccagacct
    20641 ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg gcgcaactgg
    20701 acagcggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa gcattcattg
    20761 aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagatcac ccgtgggcga
    20821 accgcgaatg cgtaccgatg gccgatctgg caggggaaaa actgctgatg ctggaagatg
    20881 gtcactgttt gcgcgatcag gcaatgggtt tctgttttga agccggggcg gatgaagata
    20941 cacacttccg cgcgaccagc ctggagacgc tgcgcaacat ggtggcggca ggtagcggga
    21001 tcactttact gcctgcgctg gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc
    21061 tgccgtgcat taagccggaa ccacgccgca ctattggcct ggtttatcgt cctggctcac
    21121 cgctgcgcag ccgctatgag cagctggcag aggccatccg cgcacgaatg gatggccatt
    21181 tcgataaagt tttaaaacag gcggtttaaa ccgtttaacg cagctacccg ataggcttcc
    21241 gccatcgtcg ggtagttaaa ggtggtgttg acgaagtact caatagtgtt gccgccacct
    21301 ttctgttcca taatcgcctg accgatatga ataatttcgg cagcgcgctc gccaaagcag
    21361 tgaataccca gaatctcttt tgtttcccga tggaacaaaa ttttcagcgt gcccacgttc
    21421 atgccgacga tttgtgcgcg tgccagatgt ttaaactggg cgcggcccac ttcatatggc
    21481 actttcattg cggtcagctg ctgttcggtt ttgcccacag agctgatttc cgggatggtg
    21541 taaatgccgg tagggatatc ttcaatcaga tgtgcggtgg cttcgccttt taccagcgcc
    21601 tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca ggctcggata accaatcacg
    21661 tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca tgctgttgac cttcagctgt
    21721 ccgcggctgt cagtttccag cccaatgttc tgtaacgcca gcgaatcggt attaccggtg
    21781 cgaccgttgg catagagcag gcagtcagct ttcagttttt tacccgactt caaatgcatg
    21841 atcacaccat cgtcacagcc ttcgatcttc tcgtactctt cgttgtgacg aatcactacg
    21901 ccactgttcc agaagtgata ggagagagaa tctgacatct cttgatcgag aaatgccagc
    21961 aggcgatcgc gggtgttgat cagatccact tttacatcca taccgcggaa gatcgacgca
    22021 tattcacagc cgatcactcc agcaccatag ataagtacat ggcgcggttc gtggtgcatg
    22081 ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga aatcaacatc tgttggatga
    22141 tatggacgag agccgcaggc aataacaaat ttttcagcgg ttagtgtttc aacgctgccg
    22201 tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc gagcgtttcc ctgcaatatt
    22261 tcacagtgat tacgttcgta aaatccctga cgcatgcgcg tttgttgatt aatcacgtta
    22321 tcggcatggt taaggatatc ggcaaaagaa gagcggagca gtcgggaatg gtcgctgtaa
    22381 agtgggtttt gattgaattc tataatgcgg ctgacggcgt gacggagagc tttcgacggg
    22441 atggtgcccc agtgggtgca accgccgcca acattttgat aacgctcgat aactgcgacg
    22501 cgcgcacctt gcttaaccag gcccattgca gcgccttcgc cgccggggcc ggaacctatt
    22561 actatggcat cgtaatcgta ggaatgtggc atggtagggc ttacctgttc ttatacataa
    22621 aagcaacaga atggtaacat tttatcgcgg gtaagccaat tgatccccgt catttatctg
    22681 gctatatcct gagcggcctt tgctttgtct gtttcttact tttgccctga cgttttattg
    22741 gatttttatc gacgatactc tccgtttaag cggcaggttt ccgctgtacg taaaagaacc
    22801 ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca atactggagc
    22861 aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca
    22921 tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg
    22981 catttagcca attaagtgct gaacgcagct tcaccagcct gagtttgcgt gaagtggcgc
    23041 gtgaagcggg cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg
    23101 gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgc caggcgcgtc
    23161 agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt atggagttca
    23221 tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg
    23281 cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc
    23341 tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga
    23401 caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat
    23461 tagaagagcg actggtactg caactgcgaa tgatttcgaa aggggcttat tactggtatc
    23521 gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca
    23581 agcaaatcaa gatagaggta cgctgctgct ggcgttagtt gctggcttat cgattaatgg
    23641 tactttcgca gcgctgttta gctccattgt gccattttct gtattcccga ttatttccct
    23701 ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat cgcactatgc cggtaggctt
    23761 gccgggtctg gcagctgcct gttttattct cggcgtactg ctgtacagca cggtagttcg
    23821 tgcggaatat ccggatatcg gctctaactt cttcccggca gtactctccg tcattatggt
    23881 gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgaat aatcggtatt
    23941 atgccggatg ctggcgcatc cagcatgggt tttacttcgc ggtcagtaat acgccgcact
    24001 ccatatggtg cgtgtagggg aactgatcaa acagagccag acgttcgacc ttgtgcgtct
    24061 ggcttaatgt ttccagattc ttgcataacg tttccgggtt acaggagatg tacaaaatac
    24121 acggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc ggtgggtcga
    24181 caaaaatggt ttcgcactga taactcttta agtcgatccc ttgcaggcgg ttaaactcgc
    24241 gcacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat
    24301 caatatggtt agctgcgatg ttgtattgcg cagcagcaac cgacggctta gcgatttcgg
    24361 tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga aaagttaccg ttgccgcagt
    24421 acagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat tccagcatct
    24481 gaatattcat cgccgcgttc ggctgggtaa agctgttttc tacctgacgg tagatcatct
    24541 ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg atccagctcg attttggttt
    24601 tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca tcgcgcaggg
    24661 cctccgcttc ctgacgccac tcatcatcca gcttcttatg gtatagcagg gaaaccaccg
    24721 cctgattact cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc agaacgggat
    24781 tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca ctggcggcgg
    24841 ggaagctatc cacgcggatg cggcttttgg tttgttgatc gaaaatgatg tgatacaggt
    24901 catcgccatc gtgccagatg cggaactccg cgcgcatccg gtaatgactg accggcgagc
    24961 gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt
    25021 tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc
    25081 ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat
    25141 gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt
    25201 gccggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa
    25261 ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt catcatctct
    25321 tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc atctggttct
    25381 catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca
    25441 atgattaaaa aagcttcgct gctgacggcg tgttctgtca cagccttttc cgcttgggca
    25501 caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc
    25561 actgtgcttg caccaaccac cgttgtgacc cgtcaggata tcgaccgctg gcagtcgacc
    25621 tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca
    25681 ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt
    25741 gatggcgtac gcctgaatct ggcgggggtg agtggttctg ccgaccttag ccagttccct
    25801 attgcgcttg tccagcgtgt tgaatatatc cgtgggccac gctccgccgt ttatggttcc
    25861 gatgcaatag gcggggtggt gaatatcatc acgacgcgcg atgaacccgg aacggaaatt
    25921 tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacgca gcaacaactg
    25981 ggggataaga cacgggtaac gctgttgggc gattatgccc atactcatgg ttatgatgtt
    26041 gttgcctatg gtaataccgg aacgcaagcg cagacagata acgatggttt tttaagtaaa
    26101 acgctttatg gcgcgctgga gcataacttt actgatgcct ggagcggctt tgtgcgcggc
    26161 tatggctatg ataaccgtac caattatgac gcgtattatt ctcccggttc accgttgctc
    26221 gatacccgta aactctatag ccaaagttgg gacgccgggc tgcgctataa cggcgaactg
    26281 attaaatcac aactcattac cagctatagc catagcaaag attacaacta cgatccccat
    26341 tatggtcgtt atgattcgtc ggcgacgctc gatgagatga agcaatacac cgtccagtgg
    26401 gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg gcagaaacag
    26461 actacgacgc cgggtacagg ttatgttgag aatggatatg atcaacgtaa taccggcatc
    26521 tatctgaccg ggctgcaaca agtcggcgat tttacctttg aaggcgcagc acgcagtgac
    26581 gataactcac agtttggtcg tcatggaacc tggcaaacca gcgccggttg ggaattcatc
    26641 gaaggttatc gcttcattgc ttcctacggg acatcttata aggcaccaaa tctggggcaa
    26701 ctgtatggct tctacggaaa tccgaatctg gacccggaga aaagcaaaca gtgggaaggc
    26761 gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc
    26821 agtgacttga tcgattatga tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg
    26881 attaagggcg tcgaggcgac cgccaatttt gataccggac cactgacgca tactgtgagt
    26941 tatgattatg tcgatgcgcg caatgcgatt accgacacgc cgttgttacg ccgtgctaaa
    27001 cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg tattacttat
    27061 cagtatttag gcactcgcta tgataaggat tactcatctt atccttatca aaccgttaaa
    27121 atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc tcatctgaca
    27181 gttcgtggta aaatagccaa cctgttcgac aaagattatg agacagtcta tggctaccaa
    27241 actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac gtcccaccgt
    27301 gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc ggcatctctt
    27361 accggatctc cattacattt atgctttcga taacgtcgct tttccgtatg gcgaaaaaag
    27421 cgaagagttt attgttgagc gagtggtggc aattgtcacc gcagtgcaag aacgttatcc
    27481 ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc gtttcacttc ctgcattacg
    27541 cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct
    27601 gacggcaaat ggcattgtcg gattactggc aacccgcgga actgttaaac gttcttatac
    27661 tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg gctcggcaga
    27721 gatggttgag ttggctgaag cgaagcttca tggcgaagat gtttctctgg atgcactaaa
    27781 acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg
    27841 cacccatttc cctctactac aagaagaact cttacaggtg ctgccagagg gaacccggct
    27901 ggtggattct ggcgcagcga ttgctcgcag aacggcctgg ctgttagaac atgaagctcc
    27961 ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc cagaagctga
    28021 acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt
    28081 aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    28141 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgtcgccg
    28201 ggtcagcggg gttctcctga gaatctcaac agagaaaagc aaaaaatgct tgactctgta
    28261 gcgggaaagc gtattgtgca caccccgcgc cgctgagaaa a
//
LOCUS       DAZFDP010000050        28251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFDP010000050 DAZFDP010000000
VERSION     DAZFDP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            424..1563
                     /gene="wza"
                     /locus_tag="ACHJ1G_002223"
     CDS             424..1563
                     /gene="wza"
                     /locus_tag="ACHJ1G_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4764072.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1569..2012
                     /gene="wzb"
                     /locus_tag="ACHJ1G_002224"
     CDS             1569..2012
                     /gene="wzb"
                     /locus_tag="ACHJ1G_002224"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4764073.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2015..4177
                     /gene="wzc"
                     /locus_tag="ACHJ1G_002225"
     CDS             2015..4177
                     /gene="wzc"
                     /locus_tag="ACHJ1G_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4764074.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4270..5109
                     /gene="wcaA"
                     /locus_tag="ACHJ1G_002226"
     CDS             4270..5109
                     /gene="wcaA"
                     /locus_tag="ACHJ1G_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4764075.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5112..5600
                     /gene="wcaB"
                     /locus_tag="ACHJ1G_002227"
     CDS             5112..5600
                     /gene="wcaB"
                     /locus_tag="ACHJ1G_002227"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4764076.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5597..6814
                     /gene="wcaC"
                     /locus_tag="ACHJ1G_002228"
     CDS             5597..6814
                     /gene="wcaC"
                     /locus_tag="ACHJ1G_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4764077.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6789..8006
                     /gene="wcaD"
                     /locus_tag="ACHJ1G_002229"
     CDS             6789..8006
                     /gene="wcaD"
                     /locus_tag="ACHJ1G_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4764078.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8017..8763
                     /gene="wcaE"
                     /locus_tag="ACHJ1G_002230"
     CDS             8017..8763
                     /gene="wcaE"
                     /locus_tag="ACHJ1G_002230"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4764079.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8779..9327
                     /gene="wcaF"
                     /locus_tag="ACHJ1G_002231"
     CDS             8779..9327
                     /gene="wcaF"
                     /locus_tag="ACHJ1G_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4764080.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9353..10474
                     /gene="gmd"
                     /locus_tag="ACHJ1G_002232"
     CDS             9353..10474
                     /gene="gmd"
                     /locus_tag="ACHJ1G_002232"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4764081.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10477..11442
                     /gene="fcl"
                     /locus_tag="ACHJ1G_002233"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10477..11442
                     /gene="fcl"
                     /locus_tag="ACHJ1G_002233"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4764082.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11445..11924
                     /gene="gmm"
                     /locus_tag="ACHJ1G_002234"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11445..11924
                     /gene="gmm"
                     /locus_tag="ACHJ1G_002234"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4764083.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11921..13144
                     /gene="wcaI"
                     /locus_tag="ACHJ1G_002235"
     CDS             11921..13144
                     /gene="wcaI"
                     /locus_tag="ACHJ1G_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4764084.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13147..14583
                     /gene="cpsB"
                     /locus_tag="ACHJ1G_002236"
     CDS             13147..14583
                     /gene="cpsB"
                     /locus_tag="ACHJ1G_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4764085.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14776..16146
                     /gene="cpsG"
                     /locus_tag="ACHJ1G_002237"
                     /gene_synonym="manB"
     CDS             14776..16146
                     /gene="cpsG"
                     /locus_tag="ACHJ1G_002237"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4764086.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16282..17676
                     /gene="wcaJ"
                     /locus_tag="ACHJ1G_002238"
     CDS             16282..17676
                     /gene="wcaJ"
                     /locus_tag="ACHJ1G_002238"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4764087.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17678..19156
                     /gene="wzxC"
                     /locus_tag="ACHJ1G_002239"
     CDS             17678..19156
                     /gene="wzxC"
                     /locus_tag="ACHJ1G_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4764088.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19302..20582
                     /gene="wcaK"
                     /locus_tag="ACHJ1G_002240"
     CDS             19302..20582
                     /gene="wcaK"
                     /locus_tag="ACHJ1G_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4764089.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            20579..21799
                     /gene="wcaL"
                     /locus_tag="ACHJ1G_002241"
     CDS             20579..21799
                     /gene="wcaL"
                     /locus_tag="ACHJ1G_002241"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4764090.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21810..23204
                     /gene="wcaM"
                     /locus_tag="ACHJ1G_002242"
     CDS             21810..23204
                     /gene="wcaM"
                     /locus_tag="ACHJ1G_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4764091.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23379..24272
                     /gene="galF"
                     /locus_tag="ACHJ1G_002243"
     CDS             23379..24272
                     /gene="galF"
                     /locus_tag="ACHJ1G_002243"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4764092.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            24644..25729
                     /gene="rfbB"
                     /locus_tag="ACHJ1G_002244"
     CDS             24644..25729
                     /gene="rfbB"
                     /locus_tag="ACHJ1G_002244"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4764093.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            25729..26628
                     /gene="rfbD"
                     /locus_tag="ACHJ1G_002245"
     CDS             25729..26628
                     /gene="rfbD"
                     /locus_tag="ACHJ1G_002245"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO4764094.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26686..27564
                     /gene="rfbA"
                     /locus_tag="ACHJ1G_002246"
     CDS             26686..27564
                     /gene="rfbA"
                     /locus_tag="ACHJ1G_002246"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4764095.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27569..28111
                     /gene="rfbC"
                     /locus_tag="ACHJ1G_002247"
     CDS             27569..28111
                     /gene="rfbC"
                     /locus_tag="ACHJ1G_002247"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO4764096.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
BASE COUNT         7086 a         6825 c         7558 g         6782 t
ORIGIN      
        1 gacacttaat ataattctta aaaatagcca attaccgaat tgttaccttg cctgctattc
       61 cgttagctgt aacacttcct cctgcattat tggagagcca atattcaatt accataatgt
      121 caccttatat tcggaagtta atttccgcaa taaccctttc tcatcaacga ttgtatgcag
      181 agagggtttg aaaaattaag ctaaatcagt gtattggtag ctaaaaagcc aggggcggta
      241 gcgtgtctgg atgcctgaaa gaccagtctg aattattctg tcaatagcct gcggatgaaa
      301 tcaatttttt taggactgat gccagttaaa tttttattca attaactgca aggtaaatgt
      361 gcatggcgac attgctatta atagtgcaca ggataattac tctgccaaag tgataaataa
      421 acaatgatga aatccaaaat gaaattgatg ccattattgg tgtcagtaac cttgataagc
      481 ggttgcacag tacttccggg cagcaatatg tcgacgatgg gcaaagacgt catcaaacag
      541 caggacgctg atttcgatct cgacaaaatg gtgaatgttt atccgctgac cccgcgcctg
      601 attgaccaat tacgcccacg cccgaatgta gcgcgcccca atatgacgct ggaaagcgag
      661 atcgcgaatt atcagtatcg cgtcgggccg ggggacgttc ttaatgtcac cgtctgggat
      721 cacccggaac tcaccacgcc agccggtcag taccgcagct ccagcgacac cggcaactgg
      781 gtacagcctg acggcactat gttttacccg tatatcggca aggttcacgt agtcgggaaa
      841 acgctcgctg aaatccgcag tgatattacc gggcgcttag cgacgtacat cgctgacccg
      901 caggtggacg ttaatatcgc cgccttccgc tcgcaaaagg cctatatctc aggtcaggtg
      961 aataaatccg gtcaacaggc gatcaccaac gtaccactga ccattctcga cgccatcaac
     1021 gccgcaggtg gcctgaccga caccgctgac tggcgcaacg tggtgctaac acacaatggt
     1081 cgtgaagaac gcatttcttt gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga
     1141 ctgctttatc ccggcgatat tctctacgtg ccgcgtaatg atgatctgaa agtatttgtg
     1201 atgggtgaag tgaagaaaca gagcaccctg aaaatggact ttagcggcat gaccctgact
     1261 gaagctctgg gcaatgccga aggcatcgac atgaccacct ccaacgccag cggcatcttt
     1321 gtcattcgtc cgttgaaagg cgagggcggg cgtaacggca agattgccaa tatctaccag
     1381 ctggatatgt ccgatgcgac gtcgctggtg atggcgacag aattccgcct gcaaccttat
     1441 gacgtggtgt atgtcaccac cgccccggtt tcccgctgga accgtctgat caatcagttg
     1501 ctgccaacta ttagcggtgt ccgttacatg acggatacag ccagcgacat tcataactgg
     1561 taatcgtcat gtttaacaac atcttagttg tctgtgtcgg caatatttgc cgttccccga
     1621 cggcggaacg cttactgcaa cgttatcacc cggagctgaa agtggagtcc gctggactcg
     1681 gcgcgctggt cggtaagggc gctgatccta ccgctatcag cgtcgccgca gaacatcaac
     1741 tgtctctgga aggtcactgt gcacggcaaa tcagccgccg cctgtgtcgc aactatgacc
     1801 tgattttgac catggaaaag cgccatatcg aacgcttatg cgagatggca cctgagatgc
     1861 gcggcaaagt gatgctgttt ggtcactggg ataacgaatg tgaaatcccc gatccgtatc
     1921 gcaaaagccg ggaaacgttt gcagcggtgt acacattact tgaacggtct gcccgccagt
     1981 gggcgcaggc attgaacgca gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc
     2041 cgctccggta acgggcagtg atgaaatcga tattggtcgc ctggtcggca ccgtcattga
     2101 agcgcgctgg tgggtgattg gcatcaccgc tgtatttgcc ctctgtgctg tggtttacac
     2161 tttcttcgcc acgccgattt atagtgccga cgcactggta caaatcgaac aaaacagcgg
     2221 caattcgtta gtgcaggaca ttggctcggc attagccaac aaaccgcctg catcggacgc
     2281 cgagatccag ttgattcgtt cgcgtctggt gcttggtaaa acggtggacg atctagacct
     2341 cgatattgcg gtgagcaaaa acacgttccc gattttcggt gcgggctggg atcgtttgat
     2401 gggacgtcag aacgagacgg tgaaagtgac cacctttaac cgcccgaaag agatggcgga
     2461 ccaggtgttt acgcttaatg tgctggacaa taaaaactac accctgagca gtgatggcgg
     2521 ctttagcgcc cgtgggcaag cgggccagat gctgaaaaaa gaaggcgtca cgctgatggt
     2581 tgaagccatt cacgcccgcc cgggcagtga gtttaccgtc accaaatact ccacgctggg
     2641 gatgatcaat caactgcaaa acagcctgac ggtaacggag aacggcaaag acgcaggcgt
     2701 actgagcctg acttataccg gtgaagatcg tgaacagatc cgcgacattc ttaacagcat
     2761 cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct
     2821 cgctttcctc gcgcaacagt taccggaagt acgtagccgc ctcgatgttg ccgaaaacaa
     2881 actgaatgcc ttccgtcagg ataaagattc tgttgatctg ccgctggaag cgaaagcggt
     2941 gctcgattcg atggtgaaca tcgacgcgca gttgaacgaa ctgaccttta aagaggcgga
     3001 aatctccaag ctgtacacca aagttcaccc cgcgtaccgc acgctgctgg agaaacgtca
     3061 ggcgctggaa gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc cgaaaaccca
     3121 gcaggaaatt gtccgtctga cccgcgatgt cgaatctggt cagcaggtct atatgcaact
     3181 gctgaataaa gagcaggagc tgaaaatcac cgaagccagc accgtcggcg atgtgcgcat
     3241 tgttgacccg gcaatcactc agcctggtgt gctaaaaccg aagaaagggc tgattatcct
     3301 cggcgcgatt atccttggcc tgatgctctc tatcgtgggt gtgctgctgc gctcgttgtt
     3361 taatcgcggc atcgaaagcc cgcaggtgct ggaagaacac ggtatcagcg tctatgccag
     3421 catcccgttg tcggaatggc agaaagcgcg cgatagcgtc aaaaccatca aagggattaa
     3481 acgctataaa cagagccagc tactggcggt ggggaatcca accgatctgg cgattgaagc
     3541 catccgcagt cttcgtacca gtttgcactt cgcgatgatg caggcgcaga acaatgtgtt
     3601 gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca acctggcggc
     3661 ggtgatcagc cagaccaata aacgcgtgtt gttgatcgac tgcgatatgc gcaaaggcta
     3721 cacccacgag ctgttgggca ctaataacgt taatggcctg tcggaaattc tgattggtca
     3781 gggcgatatt actaccgctg ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg
     3841 cggtcaggtg ccgccaaatc cttctgaact gttgatgagc gaacgctttg ccgaactggt
     3901 gaactgggcg agtaaaaact atgacctggt gttgattgat acgccgccga ttctggcagt
     3961 gaccgatgcg gcaattgttg gtcgtcatgt tggaaccacg ttaatggtgg cgcgttatgc
     4021 ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa atggtattcc
     4081 ggtgaaaggg gtgattctga actccatctt ccgccgcgcc agcgcgtatc aggattatgg
     4141 ctattacgaa tacgaatata agtcggatgc gaaataacga cgcctgcatt cgcaccgccc
     4201 cgtaggccgg ataaggcgct cacgccgcat ccggcaagca aaccagctca taagccggga
     4261 gaacaaccta tgaaagacaa tccgctgatc tcaatctata tgccaacctg gaaccgccaa
     4321 caactggcga ttcgggcaat aaaatcggtt ttgcgccagg actacagcaa ctgggagatg
     4381 atcatcgtgg atgattgctc cacctcctgg gaacaactgc aacagtacgt caccgccctc
     4441 aacgatccgc gtatcactta cattcacaac gatattaaca gcggggcgtg cgcggtacgt
     4501 aaccaggcga ttatgctggc gcaaggggaa tatatcaccg ggattgatga cgatgacgaa
     4561 tggacaccca accgtctgag cgtcttcctc gcccataaac agcaactggt cacacacgcc
     4621 tttttgtacg ctaacgacta cgtatgccag ggagaagttt attcccaacc ggcaagctta
     4681 ccactgtatc ccaaatcgcc gtactcacgc cgcctgttct ataaacgcaa tatcattggc
     4741 aatcaggtct ttacctgggc atggcgtttc aaagagtgtt tgttcgatac cgaactgaaa
     4801 gcagcacagg attacgacat cttcctgcgg atggtggtgg agtacggcga accgtggaaa
     4861 gtggaagagg cgacgcagat cctgcatatc aatcacggtg aaatgcagat cacctcgtca
     4921 ccgaaaaaat tctccgggta cttccacttt taccgcaagc acaaagataa atttgaccgt
     4981 gcaagcaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg tatgacctgg
     5041 cgcactttgc tgacgctgct gtcggtgcgt aacggcaaac gtctggctga tggtattcgg
     5101 gggcgctaaa gatgctggaa gatctgcgtg ccaacagctg gagtttacgc ccgtgctgca
     5161 tggttcttgc ctatcgtgtt gctcattttt gttcggtgtg gcgcaaaaag aacgtcctca
     5221 acaatctgtg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa tgctttttcg
     5281 gttatgaaat ccaggccgcc gcgaccattg gccgccgctt tactatccat cacggttacg
     5341 ccgtagtgat caataaaaac gtggtagcgg gggatgattt caccattcgc cacggcgtca
     5401 ctatcggcaa tcgtggtgcc gataacatgg catgtccaca cattggcaac ggcgtcgaac
     5461 tcggtgccaa cgtcattatt cttggtgata tcacgcttgg taacaacgtc accgtgggcg
     5521 cgggcagcgt ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga gaaaaagcgc
     5581 gagtgaaggt aattaaatga atattctgca atttaatgtg cgactggcgg aaggcggggc
     5641 ggcaggtgtg gcgttagatc tccatcagcg tgcgctgcaa caggggctgg cgtcacattt
     5701 tgtctacggc tacggcaaag gcggcaaaga gagcgtcagc catcaaaact atccgcaggt
     5761 cgtcaaacat acgccgcgga tgaccgcgat ggcgaacatt gcgttgtttc gtctgtttaa
     5821 tcgcgatctg tttggcaatt tcaatgagtt atatcgcacc attacttgca caccgggtcc
     5881 ggtggtcctg cattttcatg tgctgcacag ctactggcta aatcttaaga gcgtggtgcg
     5941 cttttgcgaa aaggtgaaaa accacaaacc ggatgtcact ctggtctgga cgctgcacga
     6001 ccactggagc gttaccgggc gctgcgcctt tactgacggt tgtgaaggct ggaaaacggg
     6061 ctgccagaaa tgcccgacct taaataatta tccgccggtg aagattgatc gggcgcacca
     6121 gctggtggcg ggcaaacgcc agttattccg tgagatgctg gcgctgggct gtcagtttat
     6181 ttcccccagc cagcatgtgg ctgacgcttt caatagcctg tacggtccag ggcgttgccg
     6241 gattatcaat aacggtattg atatggcaac cgaagcgatt ctggcggatc tgcctccggt
     6301 gcgcgaaacc cagggtaagc cgaaaatcgc ggtggtggcg catgacctgc gttacgacgg
     6361 caaaactaac cagcaactgg tgcgtgagat gatggcgctg ggcgacaaaa tcgaactgca
     6421 tacctttggt aagttctcgc cgttcaccgc tggcaacgtg gttaatcacg gctttgaaac
     6481 tgacaagcgc aagttgatga gcgcgctcaa tcagatggat gcgctggtgt tcagttctcg
     6541 cgtcgataac tacccgctga ttttgtgtga ggcgctatcg attggcgtgc cggtgattgc
     6601 cacccatagc gacgcggcgc gggaagtgct gcaaaaatcc ggcggtaaaa ccgtcagcga
     6661 agaagacgtg ctgcaactgg tgcagttaag caaaccggaa atcgcgcagg cgatatttgg
     6721 taccacgctg gctgagttca gccaacgcag ccgcgcggcc tacagtggac aacagatgct
     6781 ggaggagtat gtcaacttct atcagaatct gtagctacct gttgttgccg ctgatttatc
     6841 tgctggttaa cgtcaaaatc gcccagcttg gcgaaagttt ccccatcacc atcgtcactt
     6901 ttttacctgt cttgttactg ctgtttttag agcgcatcag cgttaaaaaa ttgatgattg
     6961 ccttagggat tggtgcggga ctcacggcgt ttaactatct gtttggtcag tcgctggatg
     7021 ccagtaaata cgtcacttca actatgctgt ttgtctatat tgtgatcatt attggcatgg
     7081 tgtggagtat tcgttttaaa acaatttcgc cacacaacca tcgcaagata ttacgtttct
     7141 tttatctggt ggtcgggttg gtggtggcgc tggcggcggt ggagatggcg caaattatcc
     7201 tcaccggcgg cagcagtatt atggagtcga tttcgaaata tctgatttac agcaacagct
     7261 atgtactgaa tttcattaaa ttcggcggca agcgcacgac agcactttat ttcgaaccgg
     7321 catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt ggtatcaaaa
     7381 cgcctaaaac cgatgctatg attctcgcag ggataatatt atccggatcg ttttcagggg
     7441 ttatgacctt tatcctgttt tatttgctgg agtgggcatt tcaatatctg aataaagagg
     7501 cgattaagaa aaagttaccg ttagcattga tttctctggc tgtattcctg gttggtgtgg
     7561 taatcgcgtt tccttatatt tccacccgtc tgggcgattt aggtacggaa ggatcgtcat
     7621 cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttgacc catattgacg
     7681 gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata tttaacggtg
     7741 cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat ttttcctggt
     7801 tcgcagtgtt tttatcgctg tggtacatgg ggaaagtgat aaaaatgatg atcaacgctt
     7861 ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggtg tcgctgtttt
     7921 ttaccggttc gatatttagc ccggaatatg catttttaat cgtctgtccg tttattttgc
     7981 gaaaagcgtt aaatattacg aggtaagaat aagaacatgt tgcttagcat aatcactgtc
     8041 gcgtttcgta acctcgaagg gatagtcaaa acacatgcct cgctggcgca tctggcgcag
     8101 gcggaagata tcagcttcga atggattgtt gtcgatggcg gttccaatga cggcacccgt
     8161 gagtatctgg aaaatctcaa tggtatctat aacctacgct ttgtcagcga gccagataac
     8221 ggtatctacg acgccatgaa caaaggcatt acgatggcac aaggcaagtt cgcgttgttt
     8281 ctcaattcgg gcgatatttt tcatcaggat gccgcaaatt ttgtccgtaa gttaaaaatg
     8341 caaaaagata acatgatgat caccggcgat gcgctgctgg attttggcga cgggcataaa
     8401 attaaacgta gcgccaaacc gggctggtat atttatcaca gcctgcccgc cagtcatcag
     8461 gcgatatttt tccctgtatc cggcttgaaa aaatggcgtt atgacctgga atataaagtt
     8521 tcttccgact acgcgctggc agccaaaatg tataaagccg gttatgcatt taaaaaactc
     8581 aatggcctgg tgtctgaatt ttccatgggt ggggtatcta ccaccaataa tatggaattg
     8641 tgtgctgacg cgaaaaaagt ccaacgacaa atattacatg tacctggctt ttgggctgaa
     8701 ttatcctggc atttacgcca acgtactacc tcaaagacga aagccttata taacaaagtc
     8761 tgaatataag gagaaccaat gcaagattta agcggtttct cggtgccgaa agggttccgg
     8821 ggcggcaacg ctattaaagt gcaattatgg tgggcagtac aggcaacaat atttgcctgg
     8881 tcgccacaag tattgtatcg ctggcgggct tttttattac gtttattcgg agcaaaaata
     8941 ggaaaaaacg tagttattcg tccgtcagta aaaattacct atccgtggaa attaacctta
     9001 ggtgattacg cgtgggtcgg cgatgacgtc aatttatata ccctcggtga aataaccatt
     9061 ggcgcacatt cggtgatatc gcaaaaaagt tatttatgca ccggtagcca cgaccatgca
     9121 agtcaacatt tcaccattaa cgccacgcct attgtgattg gcgagaaatg ctggctggca
     9181 accgatgtct ttgttgcccc aggcgtcaca atcggcaacg gcaccgtcgt gggtgcacga
     9241 agcagtgttt ttaaatcgct tccggcaaat gtggtttgcc gggggaatcc cgcagtggtg
     9301 atacgcgaac gcgttgaaac tgaataaatt caaaaataca gaggaataag acatgtcaaa
     9361 agtcgctctc atcaccggtg taaccggaca agacggttct tacctggcag agtttctgct
     9421 ggaaaaaggt tacgaggtgc atggtattaa gcgtcgcgca tcgtcattca acaccgagcg
     9481 cgtggatcac atttatcagg atccgcacac ctgcaacccg aaattccatc tgcattatgg
     9541 cgacctgagt gatacctcta acctgacgcg cattttgcgt gaagtacagc cggatgaagt
     9601 gtacaacctg ggcgcaatga gccacgttgc ggtctctttt gagtcaccag aatataccgc
     9661 tgacgtcgac gcgatgggta cgctgcgcct gctggaggcg atccgcttcc tcggtctgga
     9721 aaagaaaact cgtttctatc aggcttccac ctctgaactg tacggtctgg tgcaggaaat
     9781 tccgcagaaa gaaaccacgc cgttctaccc gcgatctccg tatgcggtcg ccaaactgta
     9841 cgcctactgg atcaccgtta actaccgcga atcctacggc atgtacgcct gtaacggtat
     9901 tctcttcaac catgaatccc cgcgccgcgg cgaaaccttc gttacccgca aaatcacccg
     9961 cgcaatcgcc aacatcgctc aggggctgga gtcgtgcctg tacctcggca atatggattc
    10021 cctgcgtgac tggggccacg ccaaagacta cgtaaaaatg cagtggatga tgctgcaaca
    10081 ggaacagcca gaagatttcg ttatcgcgac cggcgttcag tactccgtac gtcagttcgt
    10141 ggaaatggcg gcagcacagc tgggcatcaa actgcgcttt gaaggcacgg gcgttgaaga
    10201 gaagggcatt gtggtttccg tcaccgggca tgacgcgccg ggcgttaaac cgggcgatgt
    10261 gattatcgcc gttgacccgc gttacttccg tccggcggaa gttgaaacgc tgctcggcga
    10321 cccgaccaaa gcgcacgaaa aactgggctg gaaaccggaa atcaccctca gagagatggt
    10381 gtctgaaatg gtggctaatg acctcgaagc ggcgaaaaaa cactctctgc tgaaatctca
    10441 cggctacgac gtggcgatcg cgctggagtc ataagcatga gtaaacaacg aatttttatc
    10501 gctggccatc gcgggatggt cggttccgcc atcaagcgac agctcgaaca gcgcggtgat
    10561 gtggaactgg tattacgcac ccgcgacgag ctgaacctgc tggacagccg cgccgtgcat
    10621 gatttctttg ccagcgaacg tattgaccag gtctatctgg cggcggcgaa agtgggcggc
    10681 attgttgcca acaacaccta tccggcggat ttcatctacc agaacatgat gattgagagc
    10741 aacatcattc acgccgcgca tcagaacgac gtgaacaaac tgctgtttct cggatcgtcc
    10801 tgcatctacc cgaaactggc aaaacagccg atggcagaaa gcgagttgtt gcagggcacg
    10861 ctggagccga ctaacgagcc ttatgctatt gccaaaatcg ccgggatcaa attgtgcgaa
    10921 tcttacaatc gccagtacgg acgagattac cgttcagtca tgccgaccaa cctgtacggg
    10981 ccacacgaca acttccaccc gagtaattcg catgtgatcc ctgcattgct gcgtcgcttc
    11041 cacgaggcga cggcacagaa tgcgccggac gtggtggtgt ggggcagcgg tacaccgatg
    11101 cgtgaattcc tgcacgtcga tgatatggcg gcggcaagca ttcatgtgat ggagctggcg
    11161 cacgaagtct ggctggagaa cacccagccg atgctgtcgc acattaacgt cggcacgggc
    11221 gttgactgca ctatccgcga gctggcgcaa accatcgcca aagtggtggg ttacaaaggt
    11281 cgggtggttt ttgatgccag caaaccggat ggcacaccgc gcaaactgct ggatgtgatg
    11341 cgcctgcatc agcttggctg gtatcacgaa atatcactgg aagcggggct tgccagcact
    11401 taccagtggt tccttgagaa tcaagaccgc tttcgggggt aatgatgttt ttacgtcagg
    11461 aagactttgc cacggtagtg cgctccacac cgcttgtctc tctcgacttt attgtcgaga
    11521 acagtcgcgg cgagtttctg cttggcaaaa gaaccaaccg cccggcgcag ggttactggt
    11581 ttgtgccggg agggcgcgtg cagaaagacg aaacgctgga agccgcattt gagcggctga
    11641 cgatggcgga actggggctg cgtctgccga taacagcagg ccagttttac ggtgtctggc
    11701 agcactttta tgacgataac ttctccggca cggatttcac cactcactat gtggtgctcg
    11761 gttttcgctt cagagtcgct gaagaagatc tgttactgcc ggatgagcag catgacgatt
    11821 accgctggct gacgccggac gcgctgctcg ccagtgacaa tgttcacgct aacagccgcg
    11881 cctattttct cgctgagaag cgtgccggag tacccggatt atgaaaatac tggtctacgg
    11941 cattaactac tcgccggagt taaccggcat cggcaaatac accggcgaga tggtggaatg
    12001 gctggcggca caaggtcatg aggtgcgggt cattaccgca ccgccttact acccgcagtg
    12061 gcaggtgggc gagaactatt ccgcctggcg ctacaaacga gaagaggggg ccgccacggt
    12121 gtggcgctgc ccgctgtatg tgccaaaaca gccgagcacc ctgaaacgcc tgttgcatct
    12181 cggcagtttt gccgtcagca gttttttccc gctgatggcg caacgtcgct ggaagccgga
    12241 gcgcattatc ggcgtagtgc caacgctgtt ttgcacgccg ggaatgcgtc tgctggcgaa
    12301 actctctggc gcgcgtaccg tgctgcatat tcaggattac gaagtagatg ccatgctggg
    12361 gctgggcctt gccggaaaag gcaaaggcgg caaagtggca cagctggcaa cggcgttcga
    12421 acgtagcgga ctacataacg tcgataacgt ctccacgatt tcgcgttcga tgatgaataa
    12481 agccatcgaa aaaggcgtgg cggcggaaaa cgtcatcttc ttccccaact ggtcggaaat
    12541 cgcccgtttt cagcatgttg cagatgccga tgttgatgcc cttcgtaacc agcttggcct
    12601 gccggataac aaaaaaatca ttctttactc cggcaatatt ggtgaaaagc aggggctgga
    12661 aaacgttatt gaagctgccg atcgcctgcg cgatgaacca ctgatttttg ccattgtcgg
    12721 gcagggtggc ggcaaagcgc gactggaaaa aatggcgcag cagcgtggac tgcacaacat
    12781 gcaatttttc ccgctgcaat cgtatgacgc tttacccgca ctgctgaaga tgggcgattg
    12841 ccatctggtg gtgcaaaaac gcggcgcggc agatgccgta ttgccgtcga aactgaccaa
    12901 tattctggcg gtaggcggta acgcggtgat taccgcagag gcccacacag aactggggga
    12961 gctttgcgaa accttttcgg gcattgcggt ttgcgtagaa ccggaatcgg tcgaggcgct
    13021 ggtggcgggg atccgtcagg cgctcctgct gcccaaacac aacacggtgg cacgtgaata
    13081 tgccgaacgc acgctcgata aagagaacgt gttacgtcaa tttataaatg atattcgggg
    13141 ataattatgg cgcagtcgaa actctatcca gttgtgatgg caggtggctc cggtagccgc
    13201 ttatggccgc tttcccgcgt actttacccc aagcagtttt tatgcctgaa aggcgatctc
    13261 accatgctgc aaaccaccat ctgccgcctg aacggcgtgg agtgcgaaag cccggtggtg
    13321 atttgcaatg agcagcaccg ctttattgtc gcggaacagc tgcgtcaact gaacaaactc
    13381 accgagaaca ttattctcga accggcaggg cgtaacactg cacctgccat tgcactggcg
    13441 gcgctggcgg caaaacgtca tagcccggag agcgacccgt taatgctggt attggcggcg
    13501 gatcatgtga ttgccgatga agacgcgttc cgtgccgccg tgcgtaatgc catgccatat
    13561 gccgaagcgg gcaagctggt gaccttcggc attgtgccgg atctacctga aaccggttat
    13621 ggctatattc gtcgcggtga agtgtctgcg ggtgagcagg atacggtggc ctttgaagtg
    13681 gcgcagtttg tcgaaaaacc gaatctggaa accgcccagg cctatgtggc aagcggcgaa
    13741 tattactgga acagcggcat gttcctgttc cgcgccggac gctatctcga agaactgaaa
    13801 aaatatcgcc cggatatcct cgatgcctgc gaaaaagcga tgagcgccgt cgatccggat
    13861 ctcgatttta ttcgcgtgga tgaagaagcg tttctcgcct gcccggaaga gtcggtggat
    13921 tacgcggtca tggaacgtac ggcagatgcc gttgtggtgc cgatggatgc gggctggagc
    13981 gatgtcggtt cctggtcttc attatgggag atcagcgccc acaccgccga gggcaacgtt
    14041 tgccacggcg atgtgattaa tcacaaaaca gaaaacagct atgtgtatgc cgaatctggc
    14101 ctcgtaacca ccgtcggggt gaaagatctg gtggtagtgc agaccaaaga tgcggtgctg
    14161 attgccgacc gtaacgcggt gcaggatgtg aaaaaagtgg tcgagcagat caaagccgat
    14221 ggtcgccatg agcatcgggt acatcgcgaa gtgtatcgtc cgtggggcaa atatgactct
    14281 atcgacgcgg gcgaccgcta ccaggtgaaa cgcatcaccg tgaaaccggg cgaaggcttg
    14341 tcggtacaga tgcaccatca ccgcgcggaa cactgggtgg tggtcgcggg aacggcaaaa
    14401 gtcactattg acggtgatat caaactgctt ggtgaaaacg agtccattta tattccgctg
    14461 ggggcgacgc actgcctgga aaacccgggg aaaattccgc tcgatttaat tgaagtgcgc
    14521 tccggctctt atctcgaaga ggatgatgtg gtgcgcttcg cggatcgcta cggacgggtg
    14581 taaacgtcgc atcaggcgct gtttgtcgga tgcggcgtga acgccttatc cgacctacgg
    14641 ttcggatttg taggcctgat aagacgcggc agcgtcgcat caggcaatga atgcgaaacc
    14701 gcgatgtaaa taacgacaaa aacaaaattg gccgtttcgg tcagggccaa ctattgcctg
    14761 aaaaagggta acgatatgaa aaaattaacc tgctttaaag cctatgatat tcgcgggaaa
    14821 ttaggcgaag aactgaatga agatattgcc tggcgcattg gtcgcgccta tggcgaattt
    14881 ctcaaaccga aaaccattgt gttaggcggt gatgtccgcc tcaccagcga aaccttaaaa
    14941 ctggcgctgg cgaaaggttt acaggatgcg ggcgtcgatg tgctggatat tggcatgtcc
    15001 ggcaccgaag agatctattt cgccacgttc catctcggcg tggatggcgg cattgaagtt
    15061 accgccagcc ataatccgat ggattataac ggcatgaagc tggttcgcga gggggctcgc
    15121 ccgatcagcg gagataccgg actgcgcgac gtccagcgtc tggctgaagc caacgacttc
    15181 cctcccgtcg atgaaaccaa acgcggtcgc tatcagcaaa tcaacctgcg tgacgcttac
    15241 gttgatcacc tgttcggtta tatcaacgtc aaaaacctca cgccgctcaa gctggtgatc
    15301 aactccggga acggcgcagc gggtccggtg gtggacgcca ttgaagcccg ctttaaagcc
    15361 ctcggcgcac ccgtggaatt aatcaaagtg cacaacacgc cggacggcaa tttccccaac
    15421 ggtattccta acccactact gccggaatgc cgcgacgaca cccgcaatgc ggtcatcaaa
    15481 cacggcgcgg atatgggcat tgcctttgat ggcgattttg accgctgctt cctgtttgac
    15541 gaaaaagggc agtttattga gggttactac attgtcggcc tgctggcaga agcgttcctc
    15601 gaaaaaaatc ccggcgcgaa gatcatccac gatcctcgtc tctcctggaa taccgttgat
    15661 gtggtgactg ccgcaggcgg caccccggta atgtcgaaaa ccggacacgc ctttattaaa
    15721 gaacgtatgc gcaaggaaga cgccatctac ggtggcgaaa tgagcgccca ccattacttc
    15781 cgtgatttcg cttactgcga cagcggcatg atcccatggc tgctggtcgc cgaactggtg
    15841 tgcctgaaag gaaaaacgct gggcgaactg gtgcgcgacc ggatggcggc gtttccggca
    15901 agcggtgaga tcaacagcaa actggcgcaa cccgttgagg cgattaaccg cgtggaacag
    15961 cactttagcc gtgaggcgct ggcggtggat cgcaccgatg gcatcagcat gacctttgcc
    16021 gactggcgct ttaatctgcg ctcctccaac accgaaccgg tggtgcggtt gaatgtggaa
    16081 tcgcgcggtg atgtaccgct gatggaagcg cgaacgcgaa ctctgctgac gttgctgaac
    16141 gagtaatgtc attactttcc ccctcaccct gacgctctac tgcctgcgga gagaagtgag
    16201 cacggaactt tcttctcccc ctcgcccctt tggggagagg gccggggtga ggggcgaggc
    16261 gacaatagaa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc gaaaaccaat
    16321 gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt tgccggacta
    16381 tggctggttt gcgaagtgag cggactgtca ttcctctaca tgcacctgtt ggtggcgttg
    16441 attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg ctcatggcgc
    16501 ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt aagcgtgatt
    16561 ttcagcgccg gactggtggc gttcaataat gatttcgaca cgcaactgaa aatctggctg
    16621 gcgtggtatg ggctgaccag catcggactg gtggtttgcc gttcatgtat tcgcattggc
    16681 gcgggctggc tgcgtaatca tggctataac aagcgtatgg tcgccgtagc gggggattta
    16741 gccgccggac aaatgctgat ggagagcttc cgtaatcagc cgtggttagg gtttgaagtg
    16801 gtgggcgttt accacgaccc gaaaccgggt ggcgtttcta acgactgggc gggcaatctg
    16861 caacagctgg tcgaggatgc gaaagcgggc aagattcata acgtctatat cgcgatgcaa
    16921 atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac tggcggacac tacctgttcg
    16981 gtgctgctga tccccgatgt ctttaccttc aacattctcc attcccgtat tgaagagatg
    17041 aacggcgtac cagtggtgcc gctgtatgac acgccgcttt ccggggttaa ccgcctgctc
    17101 aaacgtgcag aagacattgt gctggcaacg ctgattctgc tgctgatctc cccggtgctg
    17161 tgctgcattg cgttggcggt gaaactgagc tcaccggggc cggttatttt ccgccagact
    17221 cgctacggca tggacggcaa gccgatcaaa gtgtggaagt tccgttcgat gaaagtgatg
    17281 gagaacgaca aagtggtgac tcaggcgacg cagaacgatc cgcgcgtcac caaagtgggg
    17341 aactttctgc gccgcacctc gctggacgaa ttgccgcagt ttatcaatgt gctgaccggg
    17401 gggatgtcga ttgtcggtcc acgtccgcac gcagtagcgc ataacgaaca gtatcgccag
    17461 ctcattgaag gctacatgct gcgccacaaa gtgaaaccgg gcattaccgg ctgggcgcag
    17521 attaatggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg cgtcgagttc
    17581 gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt tttcctgacg
    17641 gtattcaaag gcttcgttaa caaagcggca tattgatatg agcttacgtg aaaaaaccat
    17701 cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc atcggcctcg ggctggtgca
    17761 aatgactgtg ctggcgcgga ttatcgacaa ccaccagttc ggtttgctga ctgtgtcgct
    17821 ggtgattatc gcgctggcag atacgctttc tgacttcggt atcgctaact ccattattca
    17881 gcgaaaagaa atcagccacc ttgaactcac cacgttgtac tggctgaacg tcgggctggg
    17941 gatcgtggcg tgtgtggcgg tatttttgtt gagtgatgcc attggcgaca tgctgaataa
    18001 cccggacctg gcaccgttga ttaaaacatt gtcactagcg tttgtggtga tcccccacgg
    18061 gcaacagttc cgcgcgttga tgcaaaaaga gctggagttc aacaaaatcg gcatgatcga
    18121 aaccagcgcg gtgctggcgg gctttacttt tacggtggtt agcgcccatt tctggccgct
    18181 ggcgatgacc gcgatcctcg gttatctggt caatagtgcg gtgagaacgc tgctgtttgg
    18241 ctactttggc cgcaaaatct atcgccccgc tctgcatttc tcgctggcgt cggtggcacc
    18301 gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc tcaacaccaa
    18361 cctttcaaca ctcgtgctgg cgcgtattct cggcgcgggc gtggctgggg gatacaacct
    18421 ggcgtacaac gtggccgttg tgccgccgat gaaactgaac ccgatcatca cccgcgtgtt
    18481 gtttccggcg tttgccaaaa ttcaggacga taccgaaaag ctgcgcgtca acttctacaa
    18541 gctgctgtcg gtagtgggga ttatcaactt tccggcgctg ctggggctga tggtggtgtc
    18601 gaataacttt gtaccgctgg tatttggcga aaagtggagc agcattattc cggtgctaca
    18661 gctgctgtgt gtggtgggtc tgctgcgctc cgtgggtaac ccgattggtt cgctgctgat
    18721 ggcgaaagcg cgggtcgata tcagctttaa attcaacgta tttaaaacat ttctgtttat
    18781 tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc ttggcttcct
    18841 gctggtgcag attatcaaca ctattctgag ctatttcgtg atgattaaac cggttcttgg
    18901 ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct cgctgccaac
    18961 gctggtggtc agttatgcgc tgggcattgt gctgaaaggg caactggcgc tggggatgct
    19021 gctggcggtg caaatagccg cgggtgtgct ggcgtttgtg gtgatgattg tgctgtcgcg
    19081 ccatccgctg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa tgaaaatgct
    19141 tttacgggcg gggtgagttg ttgttttcgg ttccgacaac ggttgccggg tgcagcgtaa
    19201 aacgccttat ccgtcctacg gttctgtgct cgcatcaggc aaaaaacggc aacgaacaca
    19261 acaaacacag cctcacctgg catcaaattt atgaggaaaa aatgaaatta cttattctgg
    19321 gcaaccacac ttgcggcaat cgtggcgaca gcgccatctt gcgcggctta cttgatgcca
    19381 tcaacatcct caatccacat gccgaagtgg acgtgatgag ccgctatcct gtcagttctt
    19441 cctggctact caaccgcccg gtaatgggcg atccgctgtt cctgcaaatg aaacaacaca
    19501 acagcgcggc gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc taccagcatc
    19561 aggtattgct ctcacgcgtc accgacagtg gcaagctgcg taatatcgcc atcgcccagg
    19621 gattcaccga cttcgtgcgc ctgctgtcag gttacgacgc cattattcag gtcggcggat
    19681 cgttttttgt cgatctctac ggcgtgccac agtttgaaca tgcactttgc acgtttatgg
    19741 cgaaaaagtc gctgtttatg attggtcaca gtgttggccc gttccaggat gagcaattta
    19801 accaactggc gaactacgtt tttggtcact gcgacgcgct gatcctgcgc gaatcggtca
    19861 gccttgaact gatgaaacgc agcaatatca ccacttcaaa agttgaacat ggcgtcgata
    19921 ccgcgtggct ggtcgatcac cacacagaag acttcaccgc cagctatgct gttcaacact
    19981 ggctggacgt tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa ctggcaccgt
    20041 tcgacaaacg tctcggcacc actcaacaag catatgaaaa agcctttgcc ggggtggtca
    20101 atcgcattct cgacgaaggc tatcaggtga ttgcgctctc cacctgtacg ggtatcgaca
    20161 gctataacaa agacgaccgc atggtggcgc tcaacctgcg ccagcacatc agcgatcctg
    20221 cccgttacca cgtagtgatg gatgaactca acgatctgga aatgggcaaa attctggggg
    20281 cctgtgaact caccatcggt acgcgcctgc actccgccat tatctcaatg aactttgcca
    20341 ccccggcgat tgccatcaat tatgaacata aatccgccgg gattatgcag cagctgggac
    20401 taccggagat ggcgattgat atccgtcatt tattagacgg cagcctgcaa gtgatggttg
    20461 cggatacctt aggccagctt ccggcactga acacgcgact taacgaagcc gtcagtcgcg
    20521 aacgtcagac gggaatgcag atggtgaaat ctgtgcttga acacatcagg gaggcgaaat
    20581 gaaggtcggc ttctttttac tgaaatttcc gctgtcgtcg gaaaccttcg tcctcaatca
    20641 aattaccgcg tttattgata tgggctttga ggtggagatt gtcgcgctgc aaaaaggcga
    20701 cacccagaac acccacgcgg catggacgaa atacaacctt gccgccagaa cccgttggtt
    20761 acaggacgaa cctacgggca aagtggcgaa actgcgccac cgtgccagcc agatcttacg
    20821 cggcattcat cgtaaaaata cctggcaggc acttaacctc aaacgctatg gtgccgagtc
    20881 gcgaaacctg attttgtctg ccatttgcgg ccaggtcgca acaccatttt atgccgatgt
    20941 ctttatcgct cattttggcc ctgcgggggt aaccgcagca aaactccgcg aactgggtgt
    21001 cattcgcggc aaaattgcca ctatcttcca cggcattgat atctccagtc gggaggtgct
    21061 caaccattac actcccgaat atcagcaact gttccgccgt ggcgatctga tgctaccaat
    21121 aagcgatctg tgggccggaa ggttgcaaaa aatgggctgc ccgagggaaa aaatcgccgt
    21181 atcgcgcatg ggcgtggaca tgacgcgttt tagcccgcgt ccggtgaaag cgcccgcaac
    21241 gccgctggaa atcatttccg tcgcacgctt aaccgagaaa aaaggcctgc atgtggcgat
    21301 tgaagcctgc cgtcagttga aagagcaggg cgtggcattt cgctatcgca tcctcggcat
    21361 tggcccgtgg gaacgacgcc tgcgcaccct catcgaacaa tatcaactgg aagatgtggt
    21421 agagatgccg ggctttaaac cgagccacga agtgaaagcg atgctcgacg acgcggatgt
    21481 cttcctgttg ccatcggtta caggtgcgga tggcgatatg gaaggtattc cggtggcact
    21541 aatggaagcg atggcggtcg gcattccggt ggtttctact ctgcatagcg gaataccgga
    21601 actggtggag gccgataaat ccggctggct ggtgcctgag aacgatgctc gcgcactggc
    21661 gcaacgactg gcggcgttta gccaactgga caccgacgaa ctggctccgg tcgtcaaacg
    21721 tgcgcgcgaa aaagtcgaac acgattttaa ccagcaggtg atcaatcgag aactcgccag
    21781 cttgctgcag gctttataga ggagaacgca tgccatttaa aaaactctcc cgacgcacct
    21841 tcctgacggc aagctcggtg cttgccttcc tccatacccc tttcgctcgc gcgcttcccg
    21901 cccgacaaag cgttaacatt aacgactaca accctcgcga ctggatcgcc tcatttaaac
    21961 aagccttcag cgaagggcaa acggtcgtcg tgcctgccgg attggtttgc ctcaatatca
    22021 acaccggcat ctttatccct cctggcaaaa cgttacacat ccttggaagc ctgcgcggca
    22081 acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag gagggcggca
    22141 gtatgcataa catcaccctg gatgtgcgcg gctctgactg caccatcaaa gggctggcga
    22201 tgagcggctt tggtccggta acgcagattt atatcggcgg caaaaacaaa cgggtcatgc
    22261 gtaacctgac catcgataac ctgaccgtta gccacgctaa ttacgccatc ttacgccagg
    22321 gatttcataa ccagattatc ggtgccaaca tcaccaattg taagttcagc gacttacagg
    22381 gcgacgccat tgaatggaat gtggcgatta acgaccgtga tattttgata tctgaccatg
    22441 tcatcgagcg catcaactgt actaacggca aaatcaattg gggcatcggc ataggtcttg
    22501 cgggaagcac ttatgataac aactatccgg aagaccaggc agtgaaaaac tttgtcgtgg
    22561 cgaacatcac gggatcggat tgtcggcagt tgatacatgt tgaaaatggt aaacattttg
    22621 ttattcgtaa tatcaaagcc tgcaatatca cgccggattt cagtaagaaa gcaggtattg
    22681 ataacgccac ggtcgctatt tacggttgtg acaatttcgt gattgataat attgaaatga
    22741 ttaatagcgc cgggatgtta atcggctatg gggtaattaa aggcaaatat ctctcgatac
    22801 cgcagaattt ccgagtgaat aatattcagc tggataatac ccaccttgct tacaaattgc
    22861 gcggcatcca aatctccgcc gggaatgctg tctcctttgt ggcgctgact aacgttgaga
    22921 tgaagcgtgc gtcgctggag ttacacaaca agccgcaaca cctttttatg cgtaatatca
    22981 aagtgatgca ggaatcctct gttggacccg cattgagcat gaacttcgac atgcgcaaag
    23041 acgttcgcgg cgtgtttatg gcgaaaaaag aaacactgct atctcttgca aatgttcatg
    23101 cggtgaatga aagagggcaa agctccgtcg atatcgacag gataaatcac catattgtta
    23161 atgtggaaaa gattaacttt agattgccgg aacggaggga gtagatttgc gaccattcct
    23221 ggaaaaatgg agccatactt aggaacaatg ctactgcaat ctacaacgaa gcggcgtaac
    23281 atcacaagta attcagtaat caattcaggg taattgatgc tggcgaaaaa aatcgaacaa
    23341 gctataattc agcaaccatt ttacaggtgg atgaaataat gacgaattta aaagcagtta
    23401 ttcctgtagc gggtcttggg atgcatatgt tgcctgccac taaggcgatt cccaaagaga
    23461 tgctaccgat cgtcgacaag ccaatgattc agtacattgt tgacgagatt gtggctgcag
    23521 ggatcaaaga aatcctcctg gtaactcatg cgtccaagaa cgcggtcgaa aaccacttcg
    23581 acacctctta tgaattagaa tctcttcttg aactgcgcgt gaagcgtcaa ctgctggcgg
    23641 aagtacagtc catctgtccg ccaggcgtga ccattatgaa cgtgcgtcag ggcgaacctt
    23701 taggtttagg ccactccatt ttgtgtgcac gacccgccat tggtgacaat ccatttgtcg
    23761 tggtactgcc ggacgttgtg atcgacgacg ccagtgccga cccgctgcgc tacaaccttg
    23821 ctgccatgat tgcgcgtttc aatgaaacgg gccgtagcca ggtgctggca aaacgtatgc
    23881 cgggtgagct ctctgaatac tctgtcatcc agaccaaaga accgctggat cgcgaaggta
    23941 aagtcagccg cattgttgaa tttatcgaaa aaccggatca gccacaaacg ctggactcag
    24001 acatcatggc cgttggtcgc tatgtacttt ctgccgatat ttggccggaa ctggaacgta
    24061 ctcagcctgg tgcatgggga cgtattcaac tgactgatgc tattgccgag ctggcgaaaa
    24121 aacagtccgt tgatgccatg ctgatgactg gggacagtta cgactgcggt aaaaaaatgg
    24181 gctatatgca ggcgttcgtg aagtatggat tacgaaacct gaaagaaggg gcgaagttcc
    24241 gtaaaggcat tgagaagctg ttaagcaaat aatgaaaatc tgaccggatg taacggttga
    24301 taagaaaatt ataacggcag tgaagattcg tgatgaaagt aatttgttgc gaatattcct
    24361 gccgttgttt tatataaaca atcagaataa caacgagtta gcaataggat tttagtcaaa
    24421 gttttccagg attttccttg tttccagagc ggattggtaa gacaattagc gtttgaattt
    24481 ttcgggttta gcgcgagtgg gtaacgctcg tcacatcgta gacatgcatg cagtgcactg
    24541 gtagctgtaa agccaggggc ggtagcgtgc attaataccc ctattaatca acctaagagc
    24601 cgcttatttc acagcatgct ctgaagtaat atggaataat aaagtgaaga tacttgttac
    24661 tggtggcgca ggatttattg gttctgctgt agttcgtcac attataaata atacgcagga
    24721 tagtgttgtt aatgtcgata aattaacgta cgccggaaac ctggaatcac ttgctgatgt
    24781 ttctgattct gaacgctatg tttttgaaca tgcggatatt tgcgatgcag ctgcaatggc
    24841 gcggattttt gctcagcatc agccggatgc agtgatgcac ctggctgctg aaagccatgt
    24901 tgaccgttca attaccggcc ctgcggcatt tattgaaacc aatattgttg gtacttatgt
    24961 ccttttggaa gccgctcgca attactggtc tgctcttgat agcgacaaga aaaatagctt
    25021 ccgttttcat catatttcta ctgacgaagt ctatggtgat ttgcctcatc ctgacgaagt
    25081 aaataataca gaagaattac ccttatttac tgagacgaca gcttacgcgc caagcagtcc
    25141 ttattccgca tccaaagcat ctagcgatca tttagtccgc gcgtggaaac gtacctatgg
    25201 tttaccgacc attgtgacta actgttcgaa taactacggc ccttatcact ttccggaaaa
    25261 attgattccg ctggtaattc ttaatgctct ggaaggtaag gcattaccta tttatggcaa
    25321 aggggatcaa attcgcgatt ggctatatgt tgaagatcat gcgcgtgcgt tatataccgt
    25381 tgtcaccgaa ggtaaagcgg gtgaaactta taacattggc ggacacaacg aaaagaaaaa
    25441 catcgatgtt gtgctgacta tttgtgattt gttggatgag attgtaccga aagagcaatc
    25501 ttatcgtgag caaattactt atgttgccga tcgtccggga cacgatcgcc gttatgcgat
    25561 tgatgctgag aagattagcc gcgaattggg ctggaaaccg caggaaacgt ttgagagcgg
    25621 gattcggaag acagtggaat ggtacctgtc caatacaaaa tgggtcgaaa atgtgaaaag
    25681 tggtgcctat cagtcatgga ttgcacagaa ctatgagggc cgtcagtaat gaatatcctc
    25741 cttttcggca aaacagggca ggtaggttgg gaactacagc gtgctctggc acctctgggt
    25801 aatttgattg ctcttgatgt tcactctact gattattgcg gtgattttag taatcctgaa
    25861 ggtgtagctg aaaccgtaag aagcattcgg cctgatatta ttgtcaacgc agccgctcac
    25921 accgcagtag acaaagcaga atcagaaccg gagtttgcac aattacttaa cgcaacaagt
    25981 gtcgaagcga ttgcgaaagc agccaatgaa gtcggcgcct ggcttattca ttactcgact
    26041 gattacgtct tccctggaaa tggcgatatg ccatggcggg agacggatgc aaccgcacca
    26101 ctaaatgttt acggtgaaac caagttagcc ggagaaaaag cgttacagga atattgcgca
    26161 aagcatctta ttttccggac cagctgggtc tatgcaggaa aaggaaataa cttcgccaaa
    26221 acgatgttac gtctggcaaa agagcgtgaa gaattagcgg ttattaatga tcagtttggt
    26281 gcgccaacag gtgctgaact gctggctgat tgtacagcac atgccattcg tgtcgcactg
    26341 aataaaccag aagtcgcagg cttgtaccat ctggtagcca gtggtaccac aacctggcac
    26401 gattatgctg cgctggtttt tgaagaggca cgcaaagcag gtattcccct tgcactcaac
    26461 aagctcaacg cagtaccaac aacagcctat cctacaccag ctcgtcgtcc gcataactct
    26521 cgccttaata cagaaaaatt tcagcagaaa tttgcgcttg ttttgcctga ttggcaggtt
    26581 ggcgtgaaac gaatgctcaa cgaattattt acgactacag caatttaata gtttttgcat
    26641 cttgttcgtg atggtggagc aagatgaatt aaaaggaatg atgaaatgaa aacgcgtaaa
    26701 ggtattattt tagctggtgg ttcgggtact cgtctttatc ctgtaactat ggctgtcagt
    26761 aaacagttgt taccgattta tgataaaccg atgatctatt acccgttgtc tacactgatg
    26821 ttagcgggtc ttcgcgatat tctgattatt agtacgccac aggatactcc tcgttttcaa
    26881 caactgctgg gtgacgggag ccagtggggg ctaaatcttc agtacaaagt gcaaccgagt
    26941 ccagatggtc ttgcgcaggc atttatcatc ggtgaagagt ttatcggtgg tgatgattgt
    27001 gctttggttc taggtgataa tatcttttac ggtcacgatc tgccgaagtt aatggatgtc
    27061 gctgttaaca aagaaagtgg tgcaacggta tttgcctatc acgttaatga tcctgaacgc
    27121 tacggtgtcg ttgagtttga taaaaacggt acggcgatca gcctggaaga aaaaccgcta
    27181 caaccaaaaa gtaattatgc ggtaaccggg ctttattttt atgataacta cgttgtcgaa
    27241 atggcgaaaa atcttaagcc ttctgcccgc ggtgaactgg aaattaccga tattaaccgt
    27301 atttatatgg aacaggggcg tttatccgtt gccatgatgg gacgtggtta tgcatggctg
    27361 gacacgggga cacatcaaag tcttattgag gcaagcaatt ttatcgcaac aatagaagaa
    27421 cgtcaggggc tgaaagtttc ctgcccggaa gaaattgctt accgtaaagg gtttatcgat
    27481 gctgagcagg tgaaagtatt agctgaaccg ttgaagaaaa atgcttatgg tcagtatctg
    27541 ctgaaaatga ttaaaggtta ttaataaaat gaacgtaatt aaaacagaaa ttccagatgt
    27601 actgattttt gaaccgaaag tttttggtga tgagcgtggt ttctttatgg aaagctttaa
    27661 tcagaaagtt tttgaagaag ctgtagggcg gaaggttgaa tttgttcagg ataatcattc
    27721 taaatcatct aagggtgtgt tacgcggact gcactatcag ctggaaccct atgctcaagg
    27781 taaattagtt cgttgtgtgg tcggtgaagt atttgatgta gcagttgata ttcgtaaatc
    27841 gtcacctaca tttgggaaat gggttggggt gaatttgtct gctgagaata agcgtcagtt
    27901 gtggatccct gaggggtttg cgcatggttt tttggtgtta agcgagatag cagagtttgt
    27961 ttataagacg acaaattatt atcatcctga atcagaaggt tgcataaaat gggatgattc
    28021 ttttttgatg attgattggc caaataaacc gatagcaatc tcagaaaaag ataaaaaagg
    28081 ctcatcaatt ttgaggctaa atgactattg atgttgaaaa aaaaacttca aaaaataaag
    28141 gaatatcatt cagtattgga gttggcaata attcagggtg cgaatgccat atttcctgtg
    28201 ttggtattcc cattttttct tattacctta ggggaaaaca tcttttcaag t
//
LOCUS       DAZFDP010000051        27765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFDP010000051 DAZFDP010000000
VERSION     DAZFDP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHJ1G_002248"
     CDS             <1..159
                     /locus_tag="ACHJ1G_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4764097.1"
                     /translation="SLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELMSDPNSS
                     MYEEEYDG"
     gene            152..757
                     /locus_tag="ACHJ1G_002249"
     CDS             152..757
                     /locus_tag="ACHJ1G_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4764098.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            754..960
                     /locus_tag="ACHJ1G_002250"
     CDS             754..960
                     /locus_tag="ACHJ1G_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO4764099.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            938..1603
                     /locus_tag="ACHJ1G_002251"
     CDS             938..1603
                     /locus_tag="ACHJ1G_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO4764100.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            1600..2223
                     /locus_tag="ACHJ1G_002252"
     CDS             1600..2223
                     /locus_tag="ACHJ1G_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4764101.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            3296..3619
                     /locus_tag="ACHJ1G_002253"
     CDS             3296..3619
                     /locus_tag="ACHJ1G_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO4764102.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3603..4079
                     /locus_tag="ACHJ1G_002254"
     CDS             3603..4079
                     /locus_tag="ACHJ1G_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4764103.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            4076..4513
                     /locus_tag="ACHJ1G_002255"
     CDS             4076..4513
                     /locus_tag="ACHJ1G_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4764104.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            4719..5237
                     /locus_tag="ACHJ1G_002256"
     CDS             4719..5237
                     /locus_tag="ACHJ1G_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4764105.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            5521..5892
                     /locus_tag="ACHJ1G_002257"
     CDS             5521..5892
                     /locus_tag="ACHJ1G_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO4764106.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            5996..6238
                     /locus_tag="ACHJ1G_002258"
     CDS             5996..6238
                     /locus_tag="ACHJ1G_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO4764107.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            6318..6743
                     /locus_tag="ACHJ1G_002259"
     CDS             6318..6743
                     /locus_tag="ACHJ1G_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO4764108.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            6740..8152
                     /locus_tag="ACHJ1G_002260"
     CDS             6740..8152
                     /locus_tag="ACHJ1G_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO4764109.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            8155..10281
                     /locus_tag="ACHJ1G_002261"
     CDS             8155..10281
                     /locus_tag="ACHJ1G_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO4764110.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYRKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVVVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            10295..11179
                     /locus_tag="ACHJ1G_002262"
     CDS             10295..11179
                     /locus_tag="ACHJ1G_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO4764111.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKHAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            11191..12462
                     /locus_tag="ACHJ1G_002263"
     CDS             11191..12462
                     /locus_tag="ACHJ1G_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO4764112.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            12505..12690
                     /locus_tag="ACHJ1G_002264"
     CDS             12505..12690
                     /locus_tag="ACHJ1G_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764113.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            12665..13147
                     /locus_tag="ACHJ1G_002265"
     CDS             12665..13147
                     /locus_tag="ACHJ1G_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO4764114.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            13156..14574
                     /locus_tag="ACHJ1G_002266"
     CDS             13156..14574
                     /locus_tag="ACHJ1G_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO4764115.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            14574..15422
                     /locus_tag="ACHJ1G_002267"
     CDS             14574..15422
                     /locus_tag="ACHJ1G_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO4764116.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            15422..15877
                     /locus_tag="ACHJ1G_002268"
     CDS             15422..15877
                     /locus_tag="ACHJ1G_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO4764117.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            15880..16572
                     /locus_tag="ACHJ1G_002269"
     CDS             15880..16572
                     /locus_tag="ACHJ1G_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4764118.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            16582..17988
                     /locus_tag="ACHJ1G_002270"
                     /pseudo
     CDS             16582..17988
                     /locus_tag="ACHJ1G_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            17988..19832
                     /locus_tag="ACHJ1G_002271"
     CDS             17988..19832
                     /locus_tag="ACHJ1G_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4764119.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(19847..20332)
                     /locus_tag="ACHJ1G_002272"
     CDS             complement(19847..20332)
                     /locus_tag="ACHJ1G_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764120.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(20403..20669)
                     /locus_tag="ACHJ1G_002273"
     CDS             complement(20403..20669)
                     /locus_tag="ACHJ1G_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4764121.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            20791..22914
                     /locus_tag="ACHJ1G_002274"
     CDS             20791..22914
                     /locus_tag="ACHJ1G_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO4764122.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHLPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            22985..24127
                     /locus_tag="ACHJ1G_002275"
     CDS             22985..24127
                     /locus_tag="ACHJ1G_002275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4764123.1"
                     /translation="MERKTNKLDSIQVLRGIAVLLVIAFHFRVYLNGVYAQKDLGDIL
                     FGIGEVGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNY
                     EAIYTSSQIIKSYLLIPIDYNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKY
                     RTAIASFLLIIICVLGQLYFHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMI
                     IAEAYIRLPSRLINKMPVVQFLNITLAIALLFLCLGLRNGPGIVAWGAYSFMIIAPIV
                     LISKCRDVYRNKILTYVGEISYSLYINHMVVYSLAVLYLKPYGVLDSKGFGVFLAMIC
                     ATFIISIISYNFIEKPSVSIGHAIARRMQKKSDVSSIKTESVREAP"
     gene            complement(24372..25529)
                     /gene="intS"
                     /locus_tag="ACHJ1G_002276"
     CDS             complement(24372..25529)
                     /gene="intS"
                     /locus_tag="ACHJ1G_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO4764124.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(25691..25765)
                     /locus_tag="ACHJ1G_002277"
     tRNA            complement(25691..25765)
                     /locus_tag="ACHJ1G_002277"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25730..25732),aa:Arg,seq:cct)
     gene            complement(25841..26773)
                     /gene="yfdC"
                     /locus_tag="ACHJ1G_002278"
     CDS             complement(25841..26773)
                     /gene="yfdC"
                     /locus_tag="ACHJ1G_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4764125.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            27067..>27765
                     /gene="mlaA"
                     /locus_tag="ACHJ1G_002279"
     CDS             27067..>27765
                     /gene="mlaA"
                     /locus_tag="ACHJ1G_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4764126.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQ"
BASE COUNT         7761 a         6307 c         6813 g         6884 t
ORIGIN      
        1 tcccttcaat accagaaaga aagcgtcgag cgagctttaa cgtgcgctaa ctgcggtcag
       61 aagctgcatg tgctggaagt tcacgtgtgt gagcactgct gtgcagaact gatgagcgat
      121 ccgaatagct caatgtacga ggaagaatac gatggctaaa ccagcgagaa ggaaatgcaa
      181 aatatgcaag gaatggtttc acccggcatt ctcaaatcag tggtggtgct gcccggaaca
      241 cggaactcaa ttagcactcg aacgacgaag taaagaacgc gaaaaagcgg aaaaagcagc
      301 agagaagaaa cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcgaaaact
      361 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagctgtaa acgccttcat
      421 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
      481 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
      541 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
      601 cgtcgaactg attaaccgca tagggcagga agcagtagac gaaatcgaat caaaccatag
      661 ccgccatcgc tggactatcg aagagtgcaa ggcgatcaag gcagagtacc aacagaaact
      721 caaagacctg cgaaatagca gaagtgaggc cgcatgacgt tctcagtaaa aaccattcca
      781 gacatgctcg ttgaagcata cggaaaccag acagaagtag cacgcagact gaaatgtagt
      841 cgcggcacgg taagaaaata cgttgatgat aaagacggga aaatgcacgc catcgtcaac
      901 gacgttctta tggttcatcg cggatggagt gaaagagatg cgctattacg aaagaattga
      961 tggcagcaaa taccgaaata tttgggtagt tggcgatctg cacggatgct acacgaacct
     1021 gatgaaaaaa ctggagacga taggattcga caccaaaaaa gacctgctta tctcggttgg
     1081 cgatttggtc gatcgcggta cagagaacgt cgaatgtctg gaattaatca cattcccctg
     1141 gttcagagct gtacgtggaa accatgagca aatgatgatt gatggcttat cagagcgtgg
     1201 aaacgtcaat cactggctgc ttaatggcgg tggctggttc tttaatctcg attacgacaa
     1261 agaaattctg gctaaagctc ttgcccataa agcaggtgaa cttccgttaa tcatcgaact
     1321 ggtgagcaaa gataaaaaat atgtcatctg ccacgccgat tatccttgtg acgaatacga
     1381 gtttggaaag ccagttgatc atcagcagtt aatttggaac cgcgaacgaa tcagcaactc
     1441 acaagacggg atcgtgaaag aaatcaaagg agcggacatg ttcatctttg gtcatacgcc
     1501 agcagtgaaa ccactcaagt ttgccaacca gatgtatatc gataccggcg cagtgttctg
     1561 cggaaacctc acattgattc atgtacaggg agaaggcgca tgagactcga aagcgtagct
     1621 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggctac ggcttctgac
     1681 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
     1741 ggaatggctg cattctgcgg taagcatgaa ctcagccaga acgacaaaca aaaggctatc
     1801 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
     1861 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
     1921 tgccgtagtg ccgcgacgcc gggcgcaaga tgcagagatt gtcacggtac aggccgtgcg
     1981 gttgatatag caaaaacaga gcagtggggg agagttgttg agaaagagtg cggaagatgc
     2041 aaaggcgtcg gctattcaag gatgccagca agtgccgcat atcgcgctgt aacgatgcta
     2101 atcccaaacc ttactcaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
     2161 gtggtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaacgc ggtcacacgt
     2221 tagcagcatg attgccacgg atggcaacat attaacggca tgatattgac ttattgaata
     2281 aagttgggta aatttgaccc aacgatgggt taattcgctc gttgtggtat tgagataaaa
     2341 agaggcggcg cttactaccg attccgccca gttggtcact tcgacgtatc gtctggaact
     2401 ccaaccatcg caggctgaga ggtctgcaaa atgcaatccc gaaacagttc gcaggtaata
     2461 gttagagcct gcataacggt ttcgggattt tttatatctg tgtaacaggt aagagcattc
     2521 tcccttatgg ggcttggctt aaatgcaccg agtgctctta tcgttgtggc agcacaacga
     2581 tagttttcgt cagagttggc gactttgcgg ttttttagaa actgaccaca aagataaatg
     2641 caaacgatga tgttgttctg atggcggcgt aatagcctgt aagtcagcaa ggtcttccga
     2701 ctccttgtaa acaaattcgg cgcactggcc cggtgtgatt aataatgggc acacaacagg
     2761 taagagcatt aaagaactgg caaagagctt aacggtctgc gaaagcattt cttagtggca
     2821 caactggccg gtacaactga gtgctctttc cggtgtggtg aatgcgcagg ctgatgcgcg
     2881 caggagagct tcggaagaac aaggtgcctg tatacaagcc ggagatcagc gccggccacc
     2941 acagccaaat ccacccagag caaaaccgtt gttcatcctt accattcact cagtattttg
     3001 ggctacacca tcagcccatt ttttaaagcg tacttccacc aagaaccaga cctaaccaac
     3061 tcattgctga cactctgtgg atacggttgt ctagtacgct ttaaaaaaga aaacccagca
     3121 tcaatggctg ggcttcgtga tatgagcggc atgtattgtt ggcgcaatcc acgcctgatt
     3181 tgctcatgaa tgcggtcacg aacaaacccg ttacaaatca accgtaaccc ggatttgttc
     3241 aagcgaccat atccataatt cctaatttga acagatcccc ttctgggggt aagacatgaa
     3301 gatgccagaa aaacatgacc tgttagccgc tattctcgcg gcaaaggaac aaggcatcgg
     3361 ggcaatcctt gcgtttgcaa tggcgtacct tcgcggcaga tataatggcg gtgcgtttac
     3421 aaaaacagta atcgacgcaa cgatgtgtgc cattatcgcc tggttcattc gtgaccttct
     3481 cgacttcgcc ggactaagta gcaatctcgc ttatataacg agcgtgttca tcggctacat
     3541 cggtactgac tcgattggtt cgcttatcaa acgcttcgct gctaaaaaag ccggagtaga
     3601 agatggtgga aatcaataat caacgcaagg cgttcctcga tatgctggcg tggtcagagg
     3661 gaacagataa tggacggcag aaaaccagaa atcatggtta tgacgtcatt gttggcggag
     3721 agctattcac tgattactcc gatcatcctc gcaaacttgt cacgctaaac ccaaaactca
     3781 aatcaacagc cgccggacgt taccagcttc tttcccgttg gtgggatgcc tatcgtaagc
     3841 agcttggcct gaaagacttc tctcccaaaa gccaggacgc tgttgcgctg cagcagatta
     3901 aagagcgtgg cgctttgccg atgattgatc gcggtgatat tcgtcaggca atcgaccgtt
     3961 gcagcaatat ctgggcttca ctgccgggcg ctggttatgg tcagttcgag cataaggctg
     4021 acagcctgat tgcaaaattc aaagaagcgg gcggaacggt cagagagatt gaggtatgag
     4081 cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc tgtcatgggc
     4141 tgttaatcat taccgtgata acgccatgac ctacaaagag cagcgcgata aagccacatc
     4201 catcatcgct gatatgcaga agcgtcaacg tgatgtagca gaactcgacg ccagatacac
     4261 aaaggagctt gctgatgcta acgctactat cgaaagcctt cgtgctgatg tttctgctgg
     4321 tcgtaagcgc ctgcaagtcg ccgccacctg tgcaaagtca acgaccggag ccagcagcat
     4381 gggcgatgga gaaagcccaa gacttacagc agatgctgaa ctcaattatt accgtctccg
     4441 aagtggaatc gacaggataa ccgcgcaggt taactacctg caggagtaca tcaggactca
     4501 gtgcctgaaa taattttttt gcaaatcaca aagtccattt aatgagcctc gcgatgcggg
     4561 gcttttttta catctgaatt tcacagcgca tctcacgcgc atattacatc acccgagcct
     4621 ttcagaaagt tgagcctgag aactgccgta tatggtggcg accatctcgg ggcggctttt
     4681 ctgtgagaca ggctcacttt ctaaaaggta aagacgctat gaatcatcaa ttggctaatc
     4741 tcgatttccg ggacatggtg gttgtttctg gtgatcgcgt gatcacaacc tcccgcaagg
     4801 tagcagctta cttcgacaag cagcatcacc acatcattca gaaaatcgaa aagctagact
     4861 gttcggatga atttctaacc agcaactttt cgcgggttac ctatgaacac aagggtaatc
     4921 agtatgttga atatgaaatt tccaaagacg gtgcgatgta catcatcatg tcgtttaccg
     4981 gcaaaaaagc tgccgccatc aaagaggcgt ttatcaaagc atttaattgg atgcgtgaca
     5041 ggctgatgga gatggctcac tcataccaaa gagagcacaa cgagttaatg ctggagttca
     5101 tgaaggaaaa ggatgttgcc agtatgtcag gacgcttgct gaaccgctgg ggcaggatca
     5161 aaaaaccgca actcatagca agaatcaaaa ggcttgagca gcaggcgcaa atatcgatcc
     5221 ccggactgcc aaagtgacca ttccaaagcc catctacggg tgggcttgat aatgaaaccg
     5281 tgatttacat ccccacaatc cgggtatgta aaagatagtt caggcgagaa cagatttaac
     5341 taaatctgtg caccaccagt tacggcagta ccgcgaaaca acccaagcca gtaagtgggg
     5401 aaataacact ggcagccact gaaagatgaa cctccagcct tatggcaaaa aagattcttt
     5461 gtggtggcga actgatggaa agacatccta atcaagcaac cactctacag ggtcataatt
     5521 atgaacgacc agcaaatcga aaaagaaatc gttgagaaag gcaaaacggc accgcgagtt
     5581 acgccagacc atatcgaagg aattattgct caggaggcat atttcacagc agaggatggt
     5641 gcctttggca aagccataaa agcgaaacat actggcggag aggtaaacta ccagtcgcac
     5701 gaatcacttt ctctgctgac gttctgcgtc ctggtgctgc gcaacggctt caccgtcacc
     5761 ggagagagtg cctgtgcaag tcaggaaaat tttgatgcag aaattggtcg gaagattgcc
     5821 cggcagaatg ctgtaaacaa aatctggatg ctcgaaggtt acttgctgaa gcagaagcta
     5881 agcgaacagt agttattaca aaagccattc cttactgagt ggctttgata atggcttata
     5941 ccctacacgg gataacttaa ctgatatccc ttttaacgga taaacggagc caacaatggc
     6001 agagattatt cccatgactg aagaacagaa attccagtta gagatttaca aactggtcat
     6061 gaaccagaac gcagccgcgg aggaagcatt tcagttcatt ggcactgacg agctgaagct
     6121 tgagctattc aaaattcact tccagtcagg tggcgctaat tcagatatca cgacccgcac
     6181 aatcgaagcg gtgcgtaaat cgaaggaagc gttagacctg ttcactaccg gagcgtaaac
     6241 atggcaactc aaggtttcga caacccatcc aaattccgcg atgaatggga taagcaagca
     6301 gaagggaaat aatcaatatg gcgactgaga aaaagaaagg tggtcgcccc tctgattata
     6361 tgccggaggt ggctaatgac atttgcgcat tgctttcctc cggtgagagt ctgcgcaaag
     6421 tttgcgaacg cccaggaatg ccgagcaaaa catcagtttt tcgctggctg gctgaacatc
     6481 aggagtttcg tgaccagtac gcgaaggcaa cagagactcg ggccgactct attttcgaag
     6541 agatattcga aattgctgac gacgtaatcc ctgatgccgc cgaggtggca aaggcaagac
     6601 ttcgcgttga tacccgcaaa tgggcgctgg cccgaatgaa tccccgtaag tatggcgaca
     6661 aggtaactaa cgagcttgtc ggcaaagacg gcggcgcaat ccagattgaa acatcaccga
     6721 tgagcactct attcggaaaa tgacctcgat taatcctatc tttgaaccgt tcattgaggc
     6781 gcatcgctac aaagtcgcca aaggcggtcg aggtagcggt aagtcatggg caattgcgag
     6841 gcttcttgtt gaagcggcgc gtcggcagcc tgtgcgtatt ctttgcgctc gtgagctgca
     6901 aaacagtatc agcgattcgg taatcaggtt gcttgaagac accatcgagc gtgaagggta
     6961 ttcggctgag tttgaaattc agcgttcaat gattcgtcat ctcggaacga atgctgaatt
     7021 catgttctac ggcatcaaaa acaacccgac gaagattaaa tcgctcgaag gcattgatat
     7081 ctgctgggtg gaagaagcgg aagcggtaac gaaggaatcg tgggatatcc tgattccaac
     7141 catccgtaag ccgttctctg aaatatgggt aagtttcaac ccgaaaaaca tcctcgacga
     7201 tacctatcag cgattcgtcg taaatcctcc cgatgatatt tgcctgctga cggtgaacta
     7261 caccgacaac ccgcattttc ctgaagtcct ccgtctggag atggaagagt gtaaacgcag
     7321 aaatccgaca ctgtatcgtc acatctggct tggtgagccg gtaagcgcaa gtgatatggc
     7381 aatcatcaaa cgtgaatggc ttgaagccgc aaccgatgcg cacacgaaac tcgggtggaa
     7441 agcgaaaggt gcggttgttt ctgcgcatga cccctcagat acagggccag atgctaaagg
     7501 ttatgcatcg cgtcacggtt cggtagttaa gcgcattgcc gaaggtctgc tgatggacat
     7561 caacgagggt gctgactggg ctacttcgct ggcgattgaa gacggcgctg accattacct
     7621 gtgggacggt gatggcgtcg gtgcgggcct acgcagacag acaacggaag cattctccgg
     7681 caagaaaatt accgccacga tgttcaaggg cagcgaatcg ccattcgatg aagatgggcc
     7741 ttatcaggcc ggagcatggg ctgatgaagt cgtacagggc gacaacgttc gcactattgg
     7801 cgatgtattc cgcaataagc gagcgcaatt ctattacgcg ctggctgaca ggctgtatct
     7861 gacatatcgg gcggttgtcc acggtgagta tgcagacccc gacgacatgc tgagcttcga
     7921 caaagaagcg ataggcgaga agatgctgga gaagctgttt gcagaactga cgcagattca
     7981 gcgcaaattc aataataacg ggaagctgga gcttatgact aaggtcgaaa tgaagcagaa
     8041 gctcggtatc ccatctccta acctggctga tgcgctgatg atgtgtatgc attgccctgc
     8101 attggtccgc gaagaaacag aaatatacgt tccctcatcc tccggttggt aaacatggca
     8161 gagacattag agaaaaaaca tgagcggatc atgctcaggt ttgatcgcgc ctattctcca
     8221 cagcaggaag tgcgcgaaaa gtgcattgaa gctacgaggt ttgctcgtgt ccccggaggt
     8281 caatgggaag gagcaacggc ggctggaact aagcttgatg agcagttcga gaagtatcct
     8341 aagtttgaaa tcaataaggt agcaactgaa cttaaccgca tcattgcaga ataccgcaat
     8401 aacagaatca ctgttaagtt tcgtcctggt gacagagagg caagcgaaga gttagccaat
     8461 aaattaaatg gtctgttccg tgctgactac gaagaaaccg atggcggtga ggcttgcgat
     8521 aatgcatttg acgacgctgc tactggtggt ttcggttgct tccgtttgac gtcgatgctg
     8581 gtcaatgaat acgaccccat ggacgatcgt cagcgtattg ctattgaacc aatatacgac
     8641 ccgtcgcgct ctgtgtggtt tgaccctgac gctaagaagt acgacaaatc tgacgcgttg
     8701 tgggcgttct gcatgtattc gttgtcacct gaaaaatatg aggctgaata cagaaagaaa
     8761 cctcctgctt ctctggatgt aacgtctatg accagttggg aatatgactg gtttgatgaa
     8821 gatgttattt acatagcgaa gtattacgaa gttcgtaaag agtctgttgt cgtcatcagt
     8881 tatcgacatc caatcactgg agagattgca acatacgaca gtgatcaggt tgaagatatt
     8941 gaagatgaac tggcaatagc tggatttcag gaagtggcaa ggcgctcagt gaagcgccgt
     9001 cgtgtgtatg tatccgtagt ggatggtgat ggtttccttg agaaacctcg acgtattcct
     9061 ggtgagcata tccccctcat cccggtttat ggaaaacgct ggttcattga tgacattgag
     9121 cgtgtcgaag gacatattgc aaaagcaatg gatccacagc gtttgtataa ccttcaggta
     9181 tcaatgctgg ctgatactgc agcgcaagac cccggtcaga tccctatagt tggcatggag
     9241 caaattcgtg gacttgagaa gcactgggag gctcgcaaca agaaacgccc agcgttcttg
     9301 ccgttgcgcg aagtgagaga taaatctggc aacattatcg ctggagctac cccggcagga
     9361 tatacacagc ctgcggttat gaatcaggca ttggctgcat tactacagca aaccagtgct
     9421 gatattcagg aggttacagg cggcagtcag gccatgcagc agatgccaag taatattgct
     9481 caggaaacgg ttaacaactt gatgaacaga gcagatatgg cttcgtttat ctatctggac
     9541 aatatggcga aaagtcttaa acgcgctggt gaagtatggc tgtcaatggc gcgtgaagtg
     9601 tacggttcag agcgtgaagt gcgcatcgtt aacgaagatg gaagtgatga tatcgctgtc
     9661 ctgagcgcac aggttgttga caggcaaaca ggggctgttg ttgcgttaaa tgacctttct
     9721 gtcggtcgat acgatgtgac ggttgatgtt ggaccaagct acacagcacg acgtgatgca
     9781 acggtttctg tactgacaaa tgtccttagc tctatgcttc caacagaccc aatgcgcccg
     9841 gcaattcagg gtattattct ggacaatatc gatggcgaag gccttgatga cttcaaagag
     9901 tacaaccgaa accaactgct gatatctggt attgcaaaac cacgcaatga gaaagagcag
     9961 cagattgttc aacaggcgca aatggcagca caaagccagc caaatcctga aatggttctc
    10021 gctcaggcgc aaatggtagc agcgcaggca gaagcgcaaa aagcaactaa cgaaactgct
    10081 caaactcaaa tcaaagcatt tactgcccag caggatgcga tggagagtca ggcaaacact
    10141 gtctataaac tggctcaagc cagaaacatc gatgacaaag cagtgatgga ggcaatacgc
    10201 cttctgaaag atgtcgccga gtcacaacaa cagcaattcc agtcaccacc acagtcaccg
    10261 gcagacttaa tgccgagtta accaggagta atcaatggaa aacgaactga tcatcgacgg
    10321 tcaggttatt gacctgtctg aaacacagga aaatgcagaa gaaaccatca tccaaacaga
    10381 gtcacagcct gagaatgaaa gccaggatga caacggaaaa gagatggcaa ctgatcctga
    10441 aaaaaccgaa gagacaccag aagattacgc cttgcgtatt ggtgatgaag aaattcagct
    10501 taacgctgac gataatgatc acattgacgg gcaacctgca ccgcaatggg tgaaagatct
    10561 tcgcaaaggc ttcaaagaaa cacagaaaga aaaccgtgag ttgcgacgcc agcttgagga
    10621 agcattagcc aagcatgcgg aacatcagca accacaacca gacgctattc caccaaaacc
    10681 gactcttgag tcgtgtgatt atgacgaaca ggcgtttgaa caggcattga ctgattggca
    10741 tgagaaaaaa ggccgtgtcg aacagcagca gcaacaaaaa ctacgtcagc aacaggaata
    10801 ccagcagcgt ttccagcaaa gggtagaagc gcataaacaa cgggcagcca aacttcctgt
    10861 gaaagattat caggaaatgg aagccattgt tcttagtgag ctaccaccaa ttcagcagga
    10921 aatcatcatt cactgtgcag acgaaggctc tgaactactc gcctatggct taggtaagag
    10981 ccagcaatta cgccagcgtg tagccgctga gacagatcca attcgcgcag cattcctctt
    11041 ggggcagatt agcaaacagg taagccttgc tccaaaacca aagaaagcca tcaagccaga
    11101 gccggaagta cgtggtggcg gtgctgatgc gaaacaagac gaattcaaca aattatgccc
    11161 cggcgcaaaa atcgaataag gaaaagataa atgcctaaca atctcgacag taacgtcagt
    11221 caaatcgttc tgaaaaaatt ccttccgggt tttatgtcag atttagttct ggcgaaaacc
    11281 gtagaccgtc agttgctggc aggtgaaatc aactccagca ctggcgatag cgttagcttt
    11341 aaacgtccgc atcaattctc atccctccgt actcccactg gtgatatttc agggcaaaat
    11401 aaaaacaacc tgatctcagg taaagctacg gggcgtgtag gtaactacat cactgttgct
    11461 gttgaatatc agcaactgga ggaagcgatc aagcttaacc agctggaaga aattctcgcg
    11521 ccggttcgcc agcgaatcgt taccgacctt gaaacagagc ttgctcactt catgatgaat
    11581 aacggtgcgt tgtcacttgg tagccccaat actccaatca ccaaatggtc tgatgttgcg
    11641 cagacggcat ctttcctgaa agacctcggc gttaatgaag gtgaaaacta tgctgtaatg
    11701 gatccatggt ctgcacagcg acttgctgat gcgcagactg gtttgcatgc ttcagatcaa
    11761 ttggttcgta ctgcatggga gaacgcacag atcccaacca attttggcgg cattcgcgca
    11821 ctgatgtcta atgggcttgc ctctcgtacg cagggggcat ttggcggaac actgacagtc
    11881 aaaacacagc caactgttac ctataacgca gttaaagact cataccagtt cactgtaaca
    11941 ttgaccggag cgacagccag cgttacaggt tttctgaaag ctggtgatca ggtcaaattc
    12001 accaatacct actggctgca acagcagacc aaacaggcgt tgtataacgg agccacacca
    12061 attagcttca ctgcaacggt tactgctgat gctaattcag acagcagtgg cgatgtgacg
    12121 gttacgcttt ctggtgttcc gatttatgac actacaaacc cgcagtacaa ctctgtaagt
    12181 cgtcaggtag aggcaggcga tgccgtatct gtagtaggca ctgctagcca gacaatgaag
    12241 ccaaacctgt tctataacaa gttcttctgt ggacttggat ctatcccact gccgaaactg
    12301 cacagtattg attctgctgt tgcaacatat gaaggtttct ccatccgcgt acataaatac
    12361 gcagatggcg atgccaacgt gcaaaaaatg cgcttcgact tactgcctgc atatgtgtgc
    12421 tttaacccac acatgggcgg tcagttcttc ggtaatccgt aataacaagg ggcttacgcc
    12481 ccttttatgt tttaaggaaa caatatggat cggatgagtg tattccttgc cgcagataac
    12541 gaatccgggc atgtacaggc cgttatcgca gaaaaagact tccagttttt cgaaaggttg
    12601 ggctttgttg cctcagttga tgaattgaaa ccgaccagta agcgaggtcg taaggcggcg
    12661 gacaatggca acagtactga caaagggtga gatcgtcctt tttgcgcttc gtaagtttgc
    12721 tattgcttct aatgcatcgc ttactgatgt tgagccgcaa tcaattgaag atggtgtaaa
    12781 tgatctggaa gatatgatgt ccgagtggat gattaacccc ggcgacattg gttacgcttt
    12841 cgcaactgga gatgagcagc cattaccaga tgatgagtca ggtcttccaa gaaaatacaa
    12901 acacgcagta ggctatcagt tattgctgag aatgctatct gattacagcc ttgaaccaac
    12961 tccgcaagtt ctcagtaacg cccaacgctc atatgatgcc ttgatgaccg acactctggt
    13021 tgttccttca atgcgacgac gtggagattt tcctgtaggg cagggtaata aatatgacgt
    13081 gttcacatct gaccgatatt atccaggcga tctccctctg attgatggcg atatcccaaa
    13141 cgcataggtg aataaatgcc tattcagcaa cttccgctta tgaaaggtgt cggcaaagac
    13201 tttagaaacg ccgactatat cgactatctg ccagtgaata tgttggctac acccaaagaa
    13261 atcctgaaca gcagcggata tcttcgctca ttcccgggca ttgccaaacg ttctgatgtg
    13321 aacggcgtat cgcgaggcgt cgagtacaac atggcacaga atgctgttta tcgcgtgtgt
    13381 ggtggcaaac tgtataaggg cgaaagtgag gtcggtgatg ttgccggaag tggtcgcgta
    13441 tcaatggcgc atggtcgaac atcacaggcg gtaggcgtta acgggcaact ggtcgaatac
    13501 cgctatgatg gcacggttaa aaccgtctca aactggcctg cagacagcgg gtttatgcag
    13561 tatgagttag gttctgttcg cgacattacg cgtttacgtg ggcgttatgc gtggtcaaaa
    13621 gacggcactg attcatggtt tatcactgac cctgaagacg aatcgcatcc tgaccgctac
    13681 agcgcacaat atcgagccga gtctcagcct gacggcatca tcggcatcgg aacatggcga
    13741 gacttcatcg tctgctttgg ttcatcgacg attgaatatt tctccctgac gggggcaacc
    13801 accgttggtg ccgctttgta tgtcgcacag ccatcgctga tggtgcagaa aggcattgcc
    13861 gggacttact gcaaaacgcc gttcgccgat tcgtatgcgt tcatcagcaa tccggcaaca
    13921 ggtgcgccgt ctgtgtatat catcggctcc ggtcaggtat caccaatcgc cagcgcgagc
    13981 attgagaaaa ttcttcgctc ctacactgct gatgaactgg ctgatggtgt gatggaatcg
    14041 ctgcgatttg atgcgcatga actgctgatt atccaccttc cgcgccacgt cctcgtgtac
    14101 gacgcatctt caagcgccaa tggtccgcaa tggtgtgtgt tgaaaacagg cctgtatgac
    14161 gatgtgtacc gcgctatcga cttcatttac gaaggaaatc agataacgtg cggcgataag
    14221 ctggaatcgg ttaccgggaa attgcagttc gatatcagca gccagtatgg gctacagcaa
    14281 gaacacctgc tgtttactcc gttgttcaaa gcggataacg ccagatgctt tgatctggag
    14341 gtggaatcat cgacgggtgt tgctcagtac gctgaccgcc tgttcctctc tgcaaccact
    14401 gacggcataa attacgggcg tgagcagatg attgagcaga atgaaccgtt cgtttacgac
    14461 aaacgcgttt tgtggaagcg agtagggcgc atcaggaaaa atgttggctt caaactgcgc
    14521 gttatcacta agtcgcctgt cactctgtct ggcgctcaga taaggattga gtaatggcgg
    14581 attcgaatct caatgtgccg gtaatcattc aggctacacg gctcgacaca tcagtccttc
    14641 cacgcaatat cttctcgcag tcgtatctgc tttacgttat cgcacagggt actgatgttg
    14701 gtaatgtggc taacaaggcc aacgaggccg gacagggcgc ttatgacgca caagtcagga
    14761 acgatgagca ggatgtgatt ctcgctgacc atgagcagcg aatttctgct gcggaagcaa
    14821 cgcttgttaa tcatgaggag cgaatcagcc aggcagaatc aactcttcag gaacatgaaa
    14881 cgcgaatcgc tcagaatgaa agcgatattg cgtcgcttga taccagagtt cagtcgctgg
    14941 aatcgcaggt ttcagaccat gaatcgcgca ttgatgctct ggagtatgcc actactcgca
    15001 agaagtcaga ggttgtttac tctggcgtat ctgtaaccat cccgacagcg ccgaccaacc
    15061 ttgttagcct gctgaaaacg ctcacgccgt catcaggctc gttggcacca ttcttcgaca
    15121 ccgttaacaa caagatggtt gtgttcaacg agaacaaaac cttgttcttc aagctgtcga
    15181 tcgtcgggac gtggcccagc ggaaccgcca acaggtcaat gcaactaaca ttttccggct
    15241 ctgttcctga cacactggta agcagtcgca actcggcgac aacgaccgat aacatcctgt
    15301 tagctacgtt cttcagcgtg gataaagacg gctttcttgc cacaaatggc agcacgttaa
    15361 ccattcagtc aaatggtgcg gcgtttactg ccacaaccat caagataatc gcggagcagt
    15421 aatgattcag ttcaaaccaa cgcgaaacat cgacctgata gaagcagtcg gaaatcaccc
    15481 tgacattatt gccggaagca acaacggtga tggatacgac tacaagcctg aatgccgtta
    15541 ctttgaggtt aacgtgtacg gtcagtttgg cggcattgtt tactatcagg aaattcagcc
    15601 gcttacattc gattgccacg ccatgtacct gccagaggtt cgtggattca gcaaggaaat
    15661 cgggctggcg ttctggcgat acattctgac taacaccacc gttcagtgcg tcacatcgtt
    15721 cgctgcgcgc aaattccgcc acggtcagat gtactgcgca atgattggcc ttaagcgtgt
    15781 aggaaccatc aagaaatact tcaaaggcgt ggatgacgtg acgttttaca gcgccacacg
    15841 cgaagaacta atcgacttcc tgaatcacgg gagatagcta tgttatatgc atttaagctg
    15901 ggcagaaaac tgcgcggcga ggaaccttat tgccctgaaa aaggcgggaa aggtggcagc
    15961 tctgataaaa gcgcaaagta tgcagcagaa gctcagaagt atgccgcaga cctgcaaaat
    16021 cagcagtggc agacgatcat gaaaaacctt gctccgttca cgccgcttgc ggagcagtat
    16081 gttaaccagc ttcagaatct ttccagttta gaaggtcagg ggcaggcact taatcagtat
    16141 tacaactctc agcagtataa agaccttgca ggtcaggctc gttaccagag tcttgctgct
    16201 gcggaggcga cgggtggact tggttcgaca gccacaagca atcaactggc tacgatcgcg
    16261 ccgacactcg gtcagtcttg gttatcaaat cagatgagca attacaacaa tctggcaaac
    16321 gttgggcttg gtgcactgca aggtcaggca aacgccgggc agacgtacgc caacaacatg
    16381 agcagcattg cacagcaaag cgcagcactt gccgctgcta atgccaataa accatcaagt
    16441 cttcagactg caattagcgg cggcacatct ggtgcgattg ccggtgcagg tcttgccagc
    16501 cttttgggaa catcaacgcc ttggggcgct ggcattggtg ctggtatcgg attgcttggc
    16561 tcgttgtttt aaggggtaat catggctact tggcaaggaa caaacggcgg attgttggct
    16621 ggtatcggcg gcgtcaactc aaacgctccg agcgtaaatg acatcggcaa tacgcttcag
    16681 cttatcaggc agaacaatga tattgatcgt tcaggcgcta acaatgttgg gctgactgct
    16741 ttgcaaggcc tttcaggtat tgcaggggtg tttcagcagg aaaagtaggc tcagcggcag
    16801 aaagaatttc agcaggcgta cgctaatgct tatgcgtctg gtgatcgcgg tgctttgcgt
    16861 cagttggcta ctcaatatcc agaccagatt gaatccgttc gtaaaggcat gggattcatt
    16921 gatgaagacc agcgtaattc tatcggcacc ttagcggctg gcgcacgcct tgcgtcatcg
    16981 tctccagaag caatgcaatc atggctgcaa aacaacgcca aggaactgac tcgcgtcggt
    17041 gttgacccta acagcgttgc tcagatgtat cagcagaacc cttcaggatt tggtgagttt
    17101 gttgatcacc ttggaatggc tgctcttggt ccaattgatt acttcaatgt tcaggacaag
    17161 atggctggtc gtgaaattga ccgaggcaga ctggcagaga caatccgcag caatcaggct
    17221 ggagaagcac ttcaggcgag agggcaaaac ctttcctatc agtcagcaat gactgggcac
    17281 aatatcgcag cacaacgctt ggctctggat cagcaagagt tcgggtttaa gatgcagcaa
    17341 gcgcaggaaa aggctcagca gttgattagc gaagcaccta agctgtcagt aaacatggaa
    17401 aaaggcatcg agacggctgt aaacaatgct acagcatcat caaactcagc caattctatg
    17461 agtgcgcttg ctcaacagtt cagagcagaa aaaccaacga caggtttgtt cggtaacgca
    17521 cagaacatgt tcgcaaaact taccggaagc gatacaacat tgcgtgattt gcgcattcgc
    17581 caaaatgccc ttgttaacag tcaggttctt aaattcctac ctcccggccc agcaacggat
    17641 aaagacgttg agatcgttcg acagggtgcg ccaactgaca tggataaccc tgagacggtc
    17701 gcaagatggc ttgatgcaat ggcaaacctt gagcgacgaa acgcgcagtt taatgagttt
    17761 aaagccgagt ggatgagcgc gaatggcaac cctggacaat cgcgtaatgg cggtcagata
    17821 ttggggttgg atgttaaaaa aggtgaatca ttggggagtg ccgttaagcg gtatatgtca
    17881 atgaatactg acgcagcgcc agcacaagat tcgacacctt caggagaacc acggaatcag
    17941 gttggatcat atacctcaaa atcaggcatt caatttacgg tggaatgatg aaagtaactg
    18001 caaacggtaa gacatttacc tttcctgatg gtacgagcac cgaagatatt ggcaccgcca
    18061 ttgatgagta ttttgctggt caggctgttc agcaacaaac agttaatcag gccaataatg
    18121 caccaacacg ggacgaacca tcattgatgc aacaagctgg cgattggctc actggtggtc
    18181 aaagtgcagg gcaaattgca gaacaggctg gtcgtggtct ggtaaacata ccatttgacg
    18241 tattgcaggg tggcgcaagt ctgattaatg caatcagtca ggggcttggt gggccaaaag
    18301 ttttggatga tgtttatcgt ccagtagaca aaccgacaga cccatacgca caagccggtg
    18361 aaacaattgg tgggtattta gttccaggag ttggaacggc aggaagcatg gctattggat
    18421 cgctggcaga ggccgcaaat cagaaaggcg atttcgcaca aaatgcagct aaaaatgccg
    18481 gagttaacct tgccgctcag ggtgttcttt ccgcagcagc aaagggaata gggcgtggaa
    18541 taacggctat aaaaggtgat attgcgccag aagtggcgaa gaaaattgcc acatcagaat
    18601 cgatgggcgt gacaccaatg acatctgatg ttatcccgcc gaaaaatgct ttcactcgcg
    18661 gccttactca ggatgccgag ggggctttgc tcgggacggg ctcaaagcga gcagagcaat
    18721 atgcaacgcg tagtaagctg gtaagcaatt attttgaccg ttttggtgag tacaaccctg
    18781 atgatgtggt gaaatctctg accaccacgt taagggggcg gaaggatgct gctggcgctg
    18841 ttatcaatga cgtcaccaat aaaatgggta atgccgcagt tgatactaca aataccatga
    18901 atgctctgaa tacagcgatc gcaagacagg aacggcttgg gacttcagcc aatcaaagcc
    18961 tgcttacatc cttgcgtaac ctgcgtgaag aattagcaaa ccctgcaact gatttggatg
    19021 ttacgtttga tctcttgcgt cagcacagaa cagcatttag atctaatgtt cagggagatg
    19081 ctatggtctt ccccaaccag gcaaaagcag ctaccaatat ggtagagaat gcaatgtcaa
    19141 aagaccttcg taacgcagtt gcaaaaaacc tcggtgcgtc agacgcagca aaatacctta
    19201 aagcaaattc cgattatgca aacgtttata ataaggtgct taataaaaac attgctaaca
    19261 agctcaacaa ggcaagcagt gaagccagtc ctgaacttat aaatactgtt gtattaagca
    19321 gaaaaccatc tgacgtgaaa cgaatctgga gcgcattgga tgataaaggg aaagatgcta
    19381 tgcgtgcagc ttacgtcagc aaaatagcgg aaaaggccgg tgactctcca gccaagttca
    19441 tcactgaagt taataagctg aaatctcagt caggtggtga aatttacaac actatttttt
    19501 ctggaaagca catgaaagag cttgattctc ttcatgaagt tctacagaaa acagcaaggt
    19561 cagacaccgc aaatgtagta actcagactg ggcaatcgca agccaacagg ataaggacga
    19621 ttggcgcaac tgcgactctt ggcgtatcaa tggggcttga ggctggtttc ggtgcaatga
    19681 tgcgcttgta tgagtccaaa gcagcaagga atgctctctt acgtttggca aacaccaaag
    19741 caggaacacc agcctatgaa agagcgctaa ataatgctgc aaatgcgata cgccctatac
    19801 tctcaagcca aattacagca gaacagcaat aaaagataag atgtaactac ctgatattac
    19861 tgctactgtt gcatgttacc gtgtttccaa atcctgaatt gcagtttgta tatgtgtcaa
    19921 cgcgtgttgg gtaaggttga gttataacag gctggcgcgc tttttgctcg atcgcttgca
    19981 ttgtgtttac agcctgataa ttcaataaag cctgctggaa tgcttggctt tgtgctattt
    20041 gttgggcttg ttcttggctt tgtaattgaa cataaagatt ctgaagctca agtcttgcct
    20101 gtgcgtcact tatcttgcct tcatcgacac ctttcccgag catctttgca gcaaggacat
    20161 acagcttagg tgttggtgct gatgccatgc gtgagtcgtt cttcacactg gcatcaaggc
    20221 aattagccat atcgctaagc ttttgatagc gttgttcgca acttgcttga tagtcactta
    20281 cttttgcgca tccaaccagc agaagcggga taattaacag tgattttttc atatggttaa
    20341 ctctccttag tttttcacag gatatcatga aggcaatgcc attttagccg gaaactagat
    20401 ttctatgttt cctttttatt attgctatac atggtcttaa gcgtttcaaa aaccattttc
    20461 ttaaccatat cagattgttg ttctgccata cgctctgcat catcaatgta aacggatgca
    20521 gagctttgtt tagccaacga ttcttcaatc gctgcaatta tctctgagtt cagcgacctg
    20581 ttattcatct tcgcgcgctg tttaattttc gcgtggagtt catgcggaag tctcaagtga
    20641 aactgcgcct cgtcgtattt gctgtacatc cttgatgcct caccagttgg gtggaatggc
    20701 atcgtaacct actggataaa tactcaatag taccatttcg gtatgcaatc acatcatggt
    20761 tgcatcatat cattcttctg gagcaatgaa atgtcagata tcaccgcaaa tgttgtggtc
    20821 agcatgcctt cgcaactctt cactatgtct cgctctttta aagccgtagc caatggaaaa
    20881 atttatatcg gtaaaattga cactgacccg gtaaatccag aaaaccagat tcaggtttat
    20941 gtggaaaacg aagacggttc tcaccttcct gtttcgcaac caatcatcat taacgctgct
    21001 ggttaccctg tatataacgg acagattgcc aagttcgtaa ctgtgcaagg ccattctatg
    21061 gctgtttatg atgcatacgg tgcgcagcag ttctattttc cgaatgtgct aaagtatgat
    21121 ccagatcagt ttaagggcaa tctcctgagc gaagatcact cacttggcga ctctctagtc
    21181 atgcataacc cacagtttgt cgatagcaca tcgaccattc tctcaaaaaa gctaactatc
    21241 gacctgagga cgcttacaga ctacgggttt gctgagggaa acaccggtgc tcagaacaaa
    21301 gctgcatttc agaaggctat tgatgatgct acgttgccaa ctgagattgt tataccagag
    21361 ggggcgttca ttgttgaccc tggtattacg attaagaata ctgtcaccat gatacgaggt
    21421 gctggcgcat atcagtccag aattttctct acgggtacag cggcaccgat aatcacgcaa
    21481 caagatgggg taattacgtt ctgcgagttc agggactttg gtcttgacgg aaatggctac
    21541 gcagctaacg gcatcagtct tactgaagcc aaccacatta aaatagaaaa tattgacgtg
    21601 gttaatacta ataacaacgc aatattagtt aacggttact ccattgacat cattgggtgc
    21661 cggttgtttc aaaacgccgg taacggtatt aatgttggtg gttactgcaa taatattaat
    21721 ataatcaaca gccgtatcta tggtaacgga gccggtggag ttttacttac gccagcttac
    21781 gctgagggcg gtatgagcgt cagagttaat ggcaacaata tagagcaaaa taagttctac
    21841 ggacttttgg cttacggggt taaaggcctt aaccttgatg ctaactattg ggaacgcaac
    21901 ggtgagattg gttacccgta tagtgtacct gagtctatta cagtacgtgc ggatatccac
    21961 cttatcgcga acaattttac attaattcct gacctttcaa aaattaatga taccgtatct
    22021 attcgcggga atcagcagac cgcgattggc tacgccagcg cgctacctaa ccaagacgga
    22081 tttattttca cgaactatgc gaaaaacctg acgattgaaa ataatcagtt attagacgca
    22141 tcgaaagtga ataatttatt agcaatgtac cacaataatc tttcgtccaa agttactgac
    22201 aggctatatc ttgccaacaa cacagtgaac tccatcggat acgttggtag ttatgacccg
    22261 gcgacacaaa accctgacac tgcgcattta atagatattg cgaacagaga gttaactgct
    22321 aactatcttg atcgaaatat gttgctctgg actgcggcat ctggaactac aggaacattg
    22381 ataaaaactc aaaacatcta tgctggtaat tattcattct tagttacgac aggagataga
    22441 gtttggggac gaacgataga cctcaacaaa tcaccagaac taaaagggaa atttgtatgg
    22501 tttggtgcgt gggtcaatga ccagggatca gcgtcaaaac ttatgtttat aattaatggt
    22561 gcaggacaaa cagactctac agcaccttta gctggtaatg ggaaatggag ctatgttagt
    22621 tgtggtgtgt atatatacga aactgataca gctataaatg ttggtataag aaattatggt
    22681 tcaggaaatg tactcataaa ctcaccttct ctatgtgcat atggaatgcc atcaaacgcg
    22741 ttgcaagttg aaaaaacaac attttatctt tcgtcagtac ctacatccgg tttctgggat
    22801 ataggtgagc gtgttatcaa tagcgcacct gcttcaggac aaccaaaggc atggacatgc
    22861 aatattccag gtgggccagg aacctattct ttcttatcag agggaaactt ttaaaagacc
    22921 tatgtattga aatgattact ttgatctggt atgtttaaaa aaaatcagta aaacacattt
    22981 tgatatggaa agaaaaacaa ataagttaga ctctattcaa gtccttagag ggatcgcggt
    23041 acttctagtc attgcgtttc actttagagt atatttaaat ggagtgtatg cccaaaagga
    23101 cttgggcgac attctttttg gtataggtga ggttggtgtt gatatattct ttgttataag
    23161 tggatttatc attacttatt cttcaatgaa taaggcaaaa aaccggccaa tggtgtttgc
    23221 tgctaagcgt ttttttaggt tgtaccctgt ttactttata atccttactc tggctatata
    23281 cctcaactat gaggcaatat atacatcatc acaaataata aagagctact tgctcatacc
    23341 aattgactat aattttatag ggccatggtc aggttatagt ataataatac ctgcatggac
    23401 actaacgtat gaaatttggt tctatgtggt tttctttttt tcattatgca tcagccataa
    23461 atatagaact gcgatcgcat catttcttct catcattatt tgcgttcttg gtcaactgta
    23521 ctttcacgga gatgtttttc taaatcctat cgcgcaaccg ccatctggtg atggaattat
    23581 tcgtaatctg gtatttgtat caaatccaat aatatatgac tttattttgg ggatgattat
    23641 tgcagaggcc tacattaggc taccttctag acttataaat aaaatgcctg ttgttcaatt
    23701 tcttaatatt acattagcga ttgccttgct ttttttatgt ctagggttaa ggaatggacc
    23761 tggaattgta gcatgggggg cttattcatt tatgattata gccccaatag tccttatttc
    23821 taagtgtagg gatgtatata gaaataaaat tctaacgtat gttggtgaaa tatcctattc
    23881 actttacatt aatcatatgg ttgtatacag tcttgctgtt ttatatctca aaccgtacgg
    23941 ggtattggat agtaaaggat ttggtgtttt tctagctatg atttgcgcta cttttattat
    24001 atcaatcata tcttataact ttattgaaaa accatcagta tccatagggc atgccatcgc
    24061 tcgtagaatg caaaaaaaat cagatgtatc ttcaattaaa actgaatccg ttagagaagc
    24121 accttgataa atagagcaat agccagatag cacctaaccg atttcgtact catagggttg
    24181 tcactttacc aaactgagag gaaatacact gataacagtt ggttatgaag tactgggtga
    24241 ggtggatgtg aatggtattt tgatgcattt cattaacctg tacgcctaag aaagcaaccc
    24301 tgaaatctaa gacacacaag ctttgcactg gattgcaagg ctttgtgcac ttcgatagtg
    24361 gttaaggtgg ttcactccac cttttcatca agccagtccg cccaccattg catcatttct
    24421 ctgcgcttat cgagatactg agcatggttg taaataccgc gcacagatcc gccgttggca
    24481 tgtgccagtt gcacttcaat agcgtcagca ggccattcgt gctcgttcat aatcgtgctg
    24541 aattcatgcc tgaatccgtg accgctttcc agaccctcat agccgatttg tttgatcaca
    24601 agtagcaccg cgttctcgca gattggcttc ttcttatcgt tgcgcccggc aaaaacaaat
    24661 tctgatactg gtttggtgat ggagcttaac gtagtgagaa gttcaaccac ctggtccgac
    24721 atcgggacaa catgaatttt gcgtcccttc atcacattgg cgtcaatggt gataatcctg
    24781 ttttcaaaat cgacgttctt ccattgcatg gaacgaagct cttttgttct tagggctgtg
    24841 tagcgtaaaa ccttggtggc aatgagcgat acgatacttc ctgaaaatgt tgccagtgct
    24901 ttattgaatg ccgggatctg gtctgcagga agaaacggga agttcttctt gcggtatccc
    24961 ctcatggcgt cagcaaggtc aggtgccggg ttatatttag ccctgccggt gacaatagcg
    25021 taacggaaaa cctcgccgca tcttctgcgg gctttatttg ctcgctccat tgcaccgcga
    25081 tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg aatttcaagg
    25141 ccgccgatga taggcaaaat gtcgtcatca aacatttttg caagttcagt cgcataccct
    25201 actgaccata cttgcttctt gtgctcgtac cattccttgt aaatcgcact aaaggaattg
    25261 ttgttagacg aagccttttt cgcctttacc ggatcgatgc caaccgagat gtctttcctc
    25321 gcagtccatg ccttatccct tgcctcctgc aaagtcatta gcggatattt tccgacggtc
    25381 aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt cccggataca
    25441 ggaacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc tttcggcttt
    25501 gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggagggtaa aattatttta
    25561 tccacttttt acccgtcatg gggtgcggct gtcaacgatc tgacgcgaac catgacgaac
    25621 tgtgaatcta cggaaggctt gatattcagg ggattttgcg gactggtacg gatgggagcg
    25681 aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta ggaggggctc
    25741 gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa tggactgcgg
    25801 ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg tttttatcat
    25861 ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta cgcttgttgc
    25921 tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg ccgccgcaga
    25981 tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag tgcagcgtgc
    26041 cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg gtgtcaccca
    26101 gtgcaataag ccaggtcatc aaaataatca ccacaatctt tgccgcaccc gctgcaggaa
    26161 acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca aacatctcgc
    26221 tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga gtttcttcat
    26281 tgaagatagg catatattca aatgcccatg cagcaatacc tgtcccgaga atattaccca
    26341 gcagcacgac gccccataat cgcataagta agccgacgtt gctcattgtc ggtttttgca
    26401 tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg gccataatga
    26461 cgataataaa accaaaggta taaccgagat tctccagtaa gaagctgcct ggcactcctt
    26521 ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac agacccgccg
    26581 caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca ccatcctggc
    26641 ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct atttctattt
    26701 ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt
    26761 tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg tagcggcttt
    26821 tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt ttgccttttt
    26881 ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg atcaaatcag
    26941 caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca cctaaacagg
    27001 cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa aaacagggag
    27061 acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct ggtggggtgt
    27121 gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt caaccgcacc
    27181 atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt cgctgtcgcc
    27241 tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac tggcaacctt
    27301 gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca ggggatggtc
    27361 cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat tgatgttgca
    27421 gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag tacgcttggt
    27481 cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag cttcacgctg
    27541 cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg gctgacctgg
    27601 ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca gctgctggat
    27661 tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga agcgtacttc
    27721 cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcag
//
LOCUS       DAZFDP010000052        27434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFDP010000052 DAZFDP010000000
VERSION     DAZFDP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHJ1G_002280"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHJ1G_002280"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4764127.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHJ1G_002281"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHJ1G_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4764128.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHJ1G_002282"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHJ1G_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4764129.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHJ1G_002283"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHJ1G_002283"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4764130.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHJ1G_002284"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHJ1G_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4764131.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHJ1G_002285"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHJ1G_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4764132.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHJ1G_002286"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHJ1G_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4764133.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHJ1G_002287"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHJ1G_002287"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4764134.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHJ1G_002288"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHJ1G_002288"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4764135.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHJ1G_002289"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHJ1G_002289"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4764136.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHJ1G_002290"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHJ1G_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4764137.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHJ1G_002291"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHJ1G_002291"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4764138.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHJ1G_002292"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHJ1G_002292"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4764139.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHJ1G_002293"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHJ1G_002293"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4764140.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHJ1G_002294"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHJ1G_002294"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4764141.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHJ1G_002295"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHJ1G_002295"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4764142.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHJ1G_002296"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHJ1G_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4764143.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHJ1G_002297"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHJ1G_002297"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4764144.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHJ1G_002298"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHJ1G_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4764145.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHJ1G_002299"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHJ1G_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4764146.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="ACHJ1G_002300"
     CDS             complement(23548..23634)
                     /locus_tag="ACHJ1G_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4764147.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHJ1G_002301"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHJ1G_002301"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4764148.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHJ1G_002302"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHJ1G_002302"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4764149.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="ACHJ1G_002303"
     CDS             25983..26027
                     /locus_tag="ACHJ1G_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764150.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHJ1G_002304"
     CDS             complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHJ1G_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4764151.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6573 a         7799 c         6570 g         6492 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
      901 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
     1141 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctacggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattt aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgca tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatct tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatag gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agtacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcattggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    13801 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    13861 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcggca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccagtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gatacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttta
    17341 acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgaactaa
    17461 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taacaatcgc ctgaccacaa tgatgttcag
    17641 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgatcc accagccccg
    17821 ccagctcatg ctgaacatcg actgccgaac cggtggtatt ttctgtcggg atcactttcc
    17881 ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 cacctttcgc ctctgcaata atggcagtca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgctgaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg tagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgcaagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcac acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatttgct gccaccagtc gcggatctca tccagtggtt
    24841 gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    25321 tactgaccga ctcaggccag acatagggta atttgttcgc cggattaaga atatctttcg
    25381 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca tgatcgatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
    26041 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa cagagagccc
    26101 acacattcag aataaaattc tacattcaat aaaaaataca gaataaacca gacattcatg
    26161 cctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct cttccatcca
    26221 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctatt
    26281 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    26341 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    26401 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    26461 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    26521 cagtaacggg cccttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
    26581 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    26641 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    26701 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    26761 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    26821 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    26881 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    26941 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    27001 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    27061 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    27121 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    27181 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
    27241 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
    27301 catttaccac agcataataa tccataatgt ttttgttgcg aaaacaatct aatcatgact
    27361 accgcgaata ctcaatcatc tacaaaatgg attaaatgtg cttatttaat aattaattta
    27421 tatatttaat gcat
//
LOCUS       DAZFDP010000053        26474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFDP010000053 DAZFDP010000000
VERSION     DAZFDP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..1271
                     /gene="nadA"
                     /locus_tag="ACHJ1G_002305"
     CDS             228..1271
                     /gene="nadA"
                     /locus_tag="ACHJ1G_002305"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4764152.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1309..2028
                     /gene="pnuC"
                     /locus_tag="ACHJ1G_002306"
     CDS             1309..2028
                     /gene="pnuC"
                     /locus_tag="ACHJ1G_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4764153.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2025..2966)
                     /gene="zitB"
                     /locus_tag="ACHJ1G_002307"
     CDS             complement(2025..2966)
                     /gene="zitB"
                     /locus_tag="ACHJ1G_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4764154.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3080..3460)
                     /locus_tag="ACHJ1G_002308"
     CDS             complement(3080..3460)
                     /locus_tag="ACHJ1G_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4764155.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3776..4828
                     /gene="aroG"
                     /locus_tag="ACHJ1G_002309"
     CDS             3776..4828
                     /gene="aroG"
                     /locus_tag="ACHJ1G_002309"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4764156.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4987..5739)
                     /gene="gpmA"
                     /locus_tag="ACHJ1G_002310"
     CDS             complement(4987..5739)
                     /gene="gpmA"
                     /locus_tag="ACHJ1G_002310"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4764157.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5942..6982)
                     /gene="galM"
                     /locus_tag="ACHJ1G_002311"
     CDS             complement(5942..6982)
                     /gene="galM"
                     /locus_tag="ACHJ1G_002311"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4764158.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6976..8124)
                     /gene="galK"
                     /locus_tag="ACHJ1G_002312"
     CDS             complement(6976..8124)
                     /gene="galK"
                     /locus_tag="ACHJ1G_002312"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4764159.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8128..9174)
                     /gene="galT"
                     /locus_tag="ACHJ1G_002313"
     CDS             complement(8128..9174)
                     /gene="galT"
                     /locus_tag="ACHJ1G_002313"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4764160.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9184..10200)
                     /gene="galE"
                     /locus_tag="ACHJ1G_002314"
     CDS             complement(9184..10200)
                     /gene="galE"
                     /locus_tag="ACHJ1G_002314"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4764161.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10461..11933)
                     /gene="modF"
                     /locus_tag="ACHJ1G_002315"
     CDS             complement(10461..11933)
                     /gene="modF"
                     /locus_tag="ACHJ1G_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4764162.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(12001..12789)
                     /gene="modE"
                     /locus_tag="ACHJ1G_002316"
     CDS             complement(12001..12789)
                     /gene="modE"
                     /locus_tag="ACHJ1G_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4764163.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12918..13067
                     /gene="acrZ"
                     /locus_tag="ACHJ1G_002317"
     CDS             12918..13067
                     /gene="acrZ"
                     /locus_tag="ACHJ1G_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4764164.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13233..14006
                     /gene="modA"
                     /locus_tag="ACHJ1G_002318"
     CDS             13233..14006
                     /gene="modA"
                     /locus_tag="ACHJ1G_002318"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4764165.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14006..14695
                     /gene="modB"
                     /locus_tag="ACHJ1G_002319"
     CDS             14006..14695
                     /gene="modB"
                     /locus_tag="ACHJ1G_002319"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4764166.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14698..15756
                     /gene="modC"
                     /locus_tag="ACHJ1G_002320"
     CDS             14698..15756
                     /gene="modC"
                     /locus_tag="ACHJ1G_002320"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4764167.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15757..16575)
                     /gene="ybhA"
                     /locus_tag="ACHJ1G_002321"
     CDS             complement(15757..16575)
                     /gene="ybhA"
                     /locus_tag="ACHJ1G_002321"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4764168.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16730..17725
                     /gene="pgl"
                     /locus_tag="ACHJ1G_002322"
     CDS             16730..17725
                     /gene="pgl"
                     /locus_tag="ACHJ1G_002322"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4764169.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17766..18719)
                     /locus_tag="ACHJ1G_002323"
     CDS             complement(17766..18719)
                     /locus_tag="ACHJ1G_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4764170.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18903..19955
                     /locus_tag="ACHJ1G_002324"
     CDS             18903..19955
                     /locus_tag="ACHJ1G_002324"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4764171.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20031..21464
                     /locus_tag="ACHJ1G_002325"
     CDS             20031..21464
                     /locus_tag="ACHJ1G_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4764172.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21647..23908
                     /locus_tag="ACHJ1G_002326"
     CDS             21647..23908
                     /locus_tag="ACHJ1G_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4764173.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24142..25425)
                     /locus_tag="ACHJ1G_002327"
     CDS             complement(24142..25425)
                     /locus_tag="ACHJ1G_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4764174.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25560..>26474)
                     /locus_tag="ACHJ1G_002328"
     CDS             complement(25560..>26474)
                     /locus_tag="ACHJ1G_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764175.1"
                     /translation="IELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKT
                     LINYMSKIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREA
                     IAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQR
                     VGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGET
                     IIASTHREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQ
                     HLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         6305 a         6528 c         6866 g         6775 t
ORIGIN      
        1 gcgctttttt gctattcagg catcctcaat ttcactttgt aaacctgatg acatcgtcag
       61 agcttactgt gcaagcaact ctacgtcggt ggaattaggc gtaattcgag tgacttttcc
      121 ccaccattcg actatcttgt ttagcatata aaacaaatta caccgataac agcgaatatt
      181 acgctaatgt cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtaatgt
      241 ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg
      301 aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat gcggtgatgg
      361 ttgcccacta ctataccgat cccgaaattc aacaactggc agaagaaacc ggtggctgta
      421 tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct actttgttag
      481 tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc
      541 tgatgccgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta
      601 acgcattttg cgatgcccat cccgatcgta ctgtcgtcgt ctacgccaac acttctgctg
      661 cggtaaaagc gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc
      721 atcttgatag tttgggtgaa aaaatcatct gggcacccga caaacatctg gggcgttacg
      781 tgcaaaaaca gacgggtgga gacattctat gctggcaggg tgcctgtatt gtgcatgatg
      841 aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg
      901 tgcatccaga atcaccacaa gctattgtcg atatggcgga tgcggtcggt tccaccagtc
      961 aactgatcgc tgctgcgaaa acattgccac atcagcggct tatcgtggca accgatcggg
     1021 gcattttcta caaaatgcag caggcagtgc cagataaaga gttactggaa gcaccaaccg
     1081 caggtgaggg tgcaacctgc cgcagctgcg cgcattgtcc gtggatggcc atgaatggcc
     1141 ttcagtccat cgcagaggca ttagaacagg aaggaagcaa tcacgaggtt catgttgatg
     1201 aaaggctgcg agagagggcg ctggtgccgc tcaatcgtat gctggatttt gcggctacac
     1261 tacgtggata acgaataata aggcgtaacg ttacgctttg ggggaaagat ggattttttt
     1321 agtgtgcaga atatcctggt acatatacca ataggggcag gcggttatga tctctcatgg
     1381 atcgaagcgg taggcacgat cgccgggttg ctgtgtattg gccttgccag tctggagaag
     1441 atcagcaact acttctttgg cctgatcaac gtcaccttgt ttggcattat tttctttcag
     1501 attcagctgt atgccagcct gctattacag gtgtttttct ttgccgcgaa tatttacggt
     1561 tggtatgcgt ggtcgcgaca aaccagtcag aacgaggcgg agttgaaaat tcgctggttg
     1621 ccattgccga aggcactcag ctggttggcg gtttgcgttg tttcgattgg tctgatgacg
     1681 gtatttatca atccggtgtt tgcatttttg acccgcctgg cagtcatgat catgcaagca
     1741 ttaggattac aggttgtgat gcctgaactg caaccggacg ctttcccgtt ctgggattca
     1801 tgcatgatgg tgttatctat cgtggcaatg attctgatga cgcgtaagta tgtggaaaac
     1861 tggctgttgt gggtgattat taacgtgatt agcgtcgtta tttttgcact tcagggcgtt
     1921 tacgccatgt ctctggagta catcatcctg acctttattg cgctcaacgg cagccggatg
     1981 tggatcaaca gcgcacgtga aagaggctca cgcgcgctgt cccattaatg gtgatgatgt
     2041 gaatggccag acaccccttc attcaggtgg cagtctggcc catgacaagg ttgatattcc
     2101 atctgaatgg tggcgtgctc aatctgatag tgatccatca ggtagtgttg gatctgatcc
     2161 aacaaggcat cgtgatcgtg tggcgggatc acctgcacat gcaacgtcat caccggcttc
     2221 tcgcctacca tccatacatg tacatggtgc acattgcgga cttccgggat ttcccggcac
     2281 atacgacgct tcagttcagc gatatccagc gataccggtg caccttcaag taattcattc
     2341 acgctatctt tcaacagtcg ccacgcgcta cgcagaacca gaagcgacac cagtatcgag
     2401 agaatggggt cagcaggtgt ccagccagtc cagataataa tcaacgcggc aataatagcc
     2461 ccaaccgaac ccagcaggtc cccgagcaca tgcagtgccg ctgctcgtac gttgaggttt
     2521 ttctcttcgc tgccgtgatg aagtaaccag aaagaaagta tatttgccag caacccagcc
     2581 acggcaattg ccatcatcat gccgccctcg accggacgcg gcgtgcggaa gcgttctatc
     2641 gcctcccaga caatcaaaat ggtaattacc acgagggcta tggcattcac aaacgccgcg
     2701 agcgtggtca gtcttagcca accgaaagtg tggcgaatgg tgggaggacg acgtgaaaat
     2761 tgcacggcga gtagggcgaa aagcagggcg gcagtatcgg tcaacatatg acccgcatcg
     2821 gccagcaatg ccagagaacc agaaagaaaa ccaccaacga cttctaccag cataaacccg
     2881 gcagtcacgc cgaaagcata caacaagcga cgagcattat tatcttctgg caggtgtgag
     2941 gacgtgtgtg agtgtgagtg cgccatgacg agtcatccct taattattct cacttaactt
     3001 tatgacatca tactgctttt agaagtgaaa aattaaaagg gagagactcc gctctcccat
     3061 tattggctat tttgcagggt tactgcgtgg taccgtcggt tttggtatcg acatcattat
     3121 tgatgccatc accggtttgt acctttttat tgatatccgg acagcgacca tctttgcaca
     3181 tggtgttctt gtgctcttca tctttggtca ttccgtcatt gttcattgaa gaaccatccg
     3241 aatgcagcat tgtgccacca gaaccggtat ttaccccgtt attgtcgacg ttatttggcg
     3301 cgacattttc acgggcgtca ggggctacct ggcccgcatc agctgcggcg tttgcctggc
     3361 cgttattagt ttgcgctccg ctatcggcag ccagtgcggc accgctggca aggcttagag
     3421 tggcagtcag aaataatgtg gccagttttg tcattttcat aggatgctcc tgttatggtc
     3481 gttatgtcgg ataacctctt ccaacagtgc atttgcaggt gaatataagg cattggttta
     3541 agatttcagc caggttatga aacgcagcag agaatcttga aataattaac aaacaaagga
     3601 gttacagtta gaaattgtag gagagatctc gtttttcgcg acaatctggc gtttttcttg
     3661 ctaattccag gattaatccg ttcatagtgt aaaaccccgt ttacacattc tgacggaaga
     3721 tatagattgg aagtattgca ttcactaaga taagtatggc aacactggaa cagacatgaa
     3781 ttatcagaac gacgatttac gcatcaaaga aatcaaagag ttacttcctc ctgtcgcatt
     3841 gctggaaaaa ttccccgcta ctgaaaatgc cgcgaatacg gttgcccatg cccgaaaagc
     3901 gatccataag atcctgaaag gtaatgatga tcgcctgttg gtggtgattg gcccatgctc
     3961 aattcatgat cctgtcgcag caaaagagta tgccactcgc ttgctggcgc tgcgtgaaga
     4021 gctgaaagat gagctggaaa tcgtaatgcg cgtctatttt gaaaagccgc gtaccacggt
     4081 gggctggaaa gggctgatta acgatccgca tatggataac agcttccaga tcaacgacgg
     4141 tctgcgtata gcccgtaaat tgctgcttga tattaacgac agcggtctgc cagcggcggg
     4201 tgagtttctc gatatgatca ccccacaata tctcgctgac ctgatgagct ggggcgcaat
     4261 tggtgcacgt accaccgaat cgcaggtgca ccgcgaactg gcgtctggtc tttcttgtcc
     4321 ggtaggtttt aaaaatggca cagacggtac gattaaagtg gctatcgatg ccattaatgc
     4381 cgccggtgcg ccgcactgct tcctgtccgt aactaaatgg gggcattcgg cgattgtgaa
     4441 taccagcggt aacggtgatt gccatatcat tctgcgcggc ggtaaagagc ctaactacag
     4501 cgcgaagcac gttgctgaag tgaaagaagg gctgaacaaa gcaggcctgc cagcacaggt
     4561 gatgatcgat ttcagccatg ctaactcgtc caaacaattc aaaaagcaga tggatgtgtg
     4621 tgctgacgtt tgccagcaga ttgccggtgg cgaaacagcc attattggcg tgatggtgga
     4681 aagccatctg gtggaaggca atcagagcct cgagagcggg gagccgctgg cctacggtaa
     4741 gagcatcacc gatgcctgca tcggctggga agataccgat gctctgttac gtcaactggc
     4801 gaatgcagta aaagcgcgtc gcgggtaagg tttaattgtc ggatgcgccg tcagagtggc
     4861 gtatccgatg aatcaccaca ggcctgataa gacgcgcagc gtcgcatcag gcaatgtgct
     4921 ccattgttag caacaaaaaa gccggctcac ttgcagtcgg ctttctcatt tttaaacgaa
     4981 tgacgtttac ttcgctttac cctggttagc aaccgccgct gctttcgctg cgatctcgtc
     5041 agcattaccc agataatagc gtttcagcgg tttgaaattc tcgtcgaact catacaccag
     5101 cggcacgccg gtcgggatat tcagctcaag aatctcttct tcactcatgt tatcaagata
     5161 tttcaccagc gcacgtaaag agttaccgtg tgcagcgatg atcacgcgct caccgctctt
     5221 catacgcggt agaatagttt cattccagta agggatcacg cggtcaatgg tcagcgccag
     5281 gctttccgtc agcggcagtt ctttctcgct cagcttcgcg taacgtggat cgtgacccgg
     5341 ataacgctca tcatctttag tcagttccgg cggagtgact gcaaaaccac gacgccactg
     5401 tttcacctgc tcgtcgccat acttttcagc ggtttctgct ttgttcagac cctgcaacgc
     5461 accgtagtga cgttcgttca gtttccagga tttctcaacg ggcagccatg cctgatccag
     5521 ttcgtccagc acattccaca gggtatggat agcgcgtttc agcacagaag tgtaagcaaa
     5581 gtcaaagctg taaccttcct ctttcagcag cttacctgct gcttttgctt cgcttacgcc
     5641 tttctcagac agatccacgt cgtaccaacc ggtgaaacgg ttttctttgt tccactgact
     5701 ttcgccatga cgaaccagaa ccagcttagt tacagccata tacttactcc tcaaatcatc
     5761 ttttaatgat aataattctc attatattgc cgtgacgaag caacagcaat gcttacgcat
     5821 aaccatagcg aaaatagtgg cgcagtgtaa ggttgttgtg aatattgagt tgcaaatatg
     5881 tcggtgtttg ctggtgattt gaacaatatg aagataaagc cctcatgacg agggcttaac
     5941 attactcagc aataaactga tattccgtca ggctggaata ctcttcgcca ggacgcagga
     6001 agcagtccgg ttgcggccat tcagggtggt tcgggctgtc cggcagaaac tcgctttcca
     6061 acgccagccc ttgccagtcg gcgtaaggtt cggttccccg cgacggtgtg ccgccgagga
     6121 agttgccgga gtagaattga agagccggag cggtggtgta gaccttcagc tgcaattttt
     6181 catctgctga ccagacatgc gccgccactt tcttgccatc gcctttggcc tgtaacaaga
     6241 atgcgtgatc gtaacctttc actttgcgct gatcgtcgtc ggcaagaaac tcactggcga
     6301 tgattttggc gctgcggaaa tcaaaagacg ttccggcgac agatttcaag ccgtcgtgcg
     6361 gaatgccgcc ttcatcaacc ggaagatatt cgtccgccag aatctgcaac ttgtgattgc
     6421 gcacgtcaga ctgctcgccg tcaagattga aatagacgtg attagtcata ttcaccgggc
     6481 aaggtttatc aactgtggcg cgataagtaa tggagatacg gttatcgtcg gtcagacgat
     6541 attgcaccgt tgcgccgaga ttacccggga agccctgatc gccatcatct gaactcaggg
     6601 caaacagcac ctgacgatcg ttctggttca caatctgcca gcgacgtttg tcgaaccctt
     6661 ccgggccgcc gtgcagctgg ttaacgccct gacttggcga aagggtcacg gtttcaccgt
     6721 caaaggtata acggctattg gcgatgcggt tggcataacg accaatagag gcccccagaa
     6781 acgcggcctg atcctgatag cattccgggc tggcacagcc gagcagcgcc tcgcggacgc
     6841 tgccatcgga aagcggaata cgggctgaaa gtaaagtcgc accccagtcc atcagcgtga
     6901 ctaccatccc tgcgttgtta cgcaaagtta acagtcggta cggctgacca tcgggtgcca
     6961 gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt
     7021 ctctttaata cctgtttttg cttcatattg ttcagcgaca gcttgctgta cggcaggcac
     7081 cagctcttcc gggatcaacg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac
     7141 gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac
     7201 ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact cgcccatacg
     7261 tttcaggtcg ccctgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag
     7321 gatatgccgc acgcgtttcg ccacgattgg gtccagctca tgtgcaacag cgttgaactc
     7381 ttcaatggtg acatcgcgca gggctggctg ctggaagaaa cgcgcaccgg tttcgcactg
     7441 ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagttac tgttgatgat
     7501 gacgacagcc acacctttcg gcatggaaac tgctttggtc cccagtgagc gacagtcaat
     7561 cagcaaggca tgatctttct tgccgagtgc ggaaattagc tgatccatga tcccgcagtt
     7621 acagccaaca aactggtttt ctgcttcctg accgttaagc gcgatttgtg cgccgtccag
     7681 cggcagataa taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga
     7741 acttaacccg gcaccctgcg gcacattgcc gctgatcacc atgtccacac cgccgaagct
     7801 gttgttacgc agttgcagat gtttcaccac gccacgaacg tagttcgccc attgatagtt
     7861 ttcatgcgcg acaatgggcg cattgaggga aaactcgtcg agctgatttt cataatcggc
     7921 tgccattacg cgaactttac ggtcatcgcg tggcgcacag ctgatcacgg tttgataatc
     7981 aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac
     8041 gcggccaggc gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga
     8101 ttgtgttttt tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct
     8161 gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag
     8221 catttcataa ccaaccataa atttacgtac ggtggcggag cgcagcagag gcggataaaa
     8281 gtgcgcgtgc agctgccagt gttgattctc ttcgccatta aatggtgcgc cgtgccagcc
     8341 catagagtag gggaaggagc actggaagag gttgtcataa cgactggtca gctttttcaa
     8401 cgccagcgcc aaatcgctgc gctgggcgtc ggtcaaatcg gtgatccgca aaacgtgggc
     8461 tttgggcagc agtagcgttt cgaacggcca ggcagcccag taaggcacaa cggctaacca
     8521 gtgttcggtt tcgacaacgg tacggctacc gtctgccagc tcgcgctgaa cataatccac
     8581 cagcattggt gatttctgtt cggcaaaata ttctttttgc aggcggtctt cgcgctcagc
     8641 ttcgttaggc aggaagctat ttgcccaaat ctgtccgtgc ggatgcgggt tagagcagcc
     8701 catcgccgca cctttgtttt caaagacctg cacccacggg tacgtttttc ccagctctgc
     8761 ggtttgctcc tgccaggttt tgacgatttc cgtcaatgcc gcaacgctca gttctggcaa
     8821 cgttttactg tgatccggtg aaaagcagat cacccggctg gtaccgcgcg cgctctggca
     8881 acgcatcaaa ggatcgttgc tttctggcgc atctggcgtg tcagacatca aggccgcaaa
     8941 gtcattagtg aaaacgtaag tcctggtgta atcggggttt ttatcgcctg tcacccgcac
     9001 attacctgcg cagaggaagc aatctggatc gtgcgcaggt aacacctgtt tggctggcgt
     9061 ttcctgcgcc ccctgccagg ggcgcttagc gcggtgcggt gaaaccagaa tccattgccc
     9121 ggtgagcggg ttgtagcggc gatgtggatg atcaacggga ttaaattgcg tcatggtcgt
     9181 tccttaatcg ggatatccct gtggatggcg tgactgccag tgccaggtgt cctgcgccat
     9241 ttcatcgagt gtgcgcgtta cgcgccagtt cagttcacgg tcggctttgc tggcgtccgc
     9301 ccagtaggcc ggaaggtcgc cctcgcgacg cggtgcaaaa tgataattaa ccggtttgcc
     9361 gcaggctttg ctgaaggcat taaccacgtc cagcacgctg ttgcctacgc cagcgccgag
     9421 gttgtagatg tgtacgcctg gcttgttcgc cagtttttcc atcgccacga cgtgaccgtc
     9481 cgccagatcc attacgtgga tgtaatcgcg tacgccagta ccatcttcgg tcggataatc
     9541 gttaccaaaa atcgccagcg agtcgcgacg gcctacagca acctgggcga tgtatggcat
     9601 caggttattc ggaatgcctt gcggatcttc gcccatatcg cccgacggat gcgcgccaac
     9661 cgggttgaag tagcgcagca gggcaatgct ccagtccggc tgggcttttt gcagatcggt
     9721 gaggatctgt tccaccatca gcttgctttt gccgtaaggg ctttgtggtg tgccggtcgg
     9781 gaagctttca acgtatggaa ttttgggctg atcgccataa acggtggcgg aggagctaaa
     9841 aataaagttt ttgacgttag cggcgcgcat ggcgctaatc aggcgcagag tgccgttgac
     9901 attgttgtcg taatattcca gcggtttttg taccgattcg ccgacggctt tcagcccggc
     9961 gaagtggatc acggtgtcga tagcgtgatc gtgcagtatc tcggtcatca acgcttcgtt
    10021 acgaatatcg ccttcaacaa acgttggatg tttgccgcct aaacgctcga taacaggcag
    10081 tacgctgcgc ttactgttac agaggttatc aagaatgatg acatcatgac cgttttgcag
    10141 taattgcaca caggtatgac ttccaatgta accgctacca ccggtaacca gaactctcat
    10201 aattcgctcc attaggctta tggtatgaaa taaccatagc ataacaaaga tgcgaaaagt
    10261 gtgacatgga ataaattagt ggaatcgttt acacaagaat ttagccgttt tttatgcgcg
    10321 attaagtgat tataaaacag agggtttatg aatgattgcg ctttttatct gaaaaaagaa
    10381 gcggtttcat gcctgcatgc gtcgaaccgt tggccggaga gggcgcttag gccgcctccg
    10441 gcaagatcag cactaccgac tcaatatatt tttgtcagca catagcgata gagtccaccg
    10501 tccggcacga actcaaggcg atgggtaata caggcaggcg catcttcagc gtggtgcgaa
    10561 acaaacaaca attgcgtttc accttcgcta atcagcacat caacaaaacg gcggataagc
    10621 tggcgattga gcggatcaag cccctgtagt ggttcatcga gaataagcaa cgtcggatgt
    10681 ttcaccagtg cgcggacgat cagcgccaga cgctgctgtc cccaggaaag actatggaac
    10741 ggagcgtcag ccgtacgttt atcaatgccg agaatatcca gccactgctg caccagtttt
    10801 tgctggcgat ccgaaacggc ctgataaatg ccaatcgaat caaaatagcc agaaagaatc
    10861 acattacgca cggtagtgct gacccggtaa tccagatgca aactactgct gacgtaaccg
    10921 atatgctttt tgatatccca gatggtttcg ccgctgccgc gacgtcgtcc gaaaagcgtc
    10981 aaatcgttgc tgtaaccttg cggatgatcg ccagtaacca ggcttaataa cgtcgatttt
    11041 cctgcaccat ttggcccgac aatttgccag tgttcacctg gattcacctg ccagctaagg
    11101 ttattaagaa tggggcgatc gttataagaa accacgccat tgttcagcac aatgcgcggt
    11161 tcgttggcgg gtaaggcgtg acgtgctgaa ggttcatccg gctccggcag ttgcacacct
    11221 tcaagctgtt cactatgcgc cagttgcgcg acgagtgctt gttggagcag ttcctcttta
    11281 gcgccagttt ccgctaacgt gcaatccgcc agcacgccag caaactggac aaactccggg
    11341 atctcatcga agcgattgag caccagtacc agagtaatac cggactgatg taacgaggcg
    11401 agtcgctcag ccagctgctg acgtgaggca acatccaggc catcgaacgg ctcatcaaga
    11461 atcaacaagt caggctccga catcagcgcc tgacacagca gggtttttcg cgtctcgcca
    11521 gtggaaaggt atttaaagcg tcggtcgagg agggcggtaa taccgaactg ctgcgccagt
    11581 tgcatgcaac gcggtgcatc ctttacttca tcctgaatga tctcagccgt agtgcgtccg
    11641 gtgtcatctt cgccagggcc gagcatatcg gtgttattcc gctgccattc gtcgctgacg
    11701 agtttttgca attgctcgaa ggagagacga gtgatgtggg aaaactggct ttgccgttca
    11761 cctttcaaaa gcggaagttc ccccgccagc gcgcgggcca gggccgattt cccgcttcca
    11821 ttcgaaccga caaacgccca actatcaccc gcgtttaacg ttagctgagg caattgcagc
    11881 gtttttgtgt cgctaagacg aaacgtgcct tgcaaaattt gcaacgatga cattttatat
    11941 cccattttgt gcagcgatta ctgacaggga tacgtgtttc ataacaaatt gtcaacacgc
    12001 ttagcacagc gtggcgataa tcacgctgtc ggcattaaag taggccgtga cattctgccc
    12061 ttgctgaaga gaagtcgctt catttaccgg cactgtggcg cacagtgttt gcccgtcggg
    12121 tagcgccatt aatacttcgc actgctctgc gccgcgctca atatgactaa taatacccgg
    12181 taattggttg tcggcgtttt gtgcgaccgc ctcgtcctga gtaataccta cccacggcgc
    12241 ttttagcaat atcaacactt ctttgccttc atccagcccc agacgcgcgc cgctttgtgc
    12301 ggtaattgcg actttcaggc gtgtttttcc gtcagccagt aagacatcaa catgctgttg
    12361 aacgtcatca tgatcgcggg cggtgatggt gccgaaccac tggttacggg cgctggtttg
    12421 cagtgaaaaa cgtgagatcg cggccagcag gctgttcagc ggcagggcgt catcgtcact
    12481 taacacatca aaggcttttt gctggatttg cgccagtaag tcatagagct gaatcagtcg
    12541 ctgaccatag cgggtcagta ctgcgccgcc gccaccttta ccgcctgttg cgcgctcgac
    12601 cagaatatgc tcacttaact gattcatctc gttaatggca tcccaggcgc ttttatagct
    12661 gataccggca tctttcgctc cctggctaat ggaaccggaa agcgcaatgt gttttagtag
    12721 cgaaatccgg cgcgggtcgg cgaataattt ttgttggagc ttaagggtga gaaggatttc
    12781 ggcctgcata acaatgtcct ggcaaaagtc ttattgtgac ggaaaacgaa cgccacgcaa
    12841 agctgaccgc acaaaagggg agtgcttttc tgtgcttagc ggttagaata gtctcatgac
    12901 tatatctgga gttgaccatg ttagagttat taaaaagtct ggtattcgcc gtaatcatgg
    12961 tacctgtcgt gatggccatc atcctgggtc tgatttacgg tcttggtgaa gtattcaaca
    13021 tcttttctgg tgttggtaaa aaagaccagc ccggacaaaa tcattgattc cctgaatgcc
    13081 cgcttagtcg ggcatttctt tttctcaact tcctgctttt ccagccgata ttttttctta
    13141 tctacctcac aaaggttagc aataactgct gggaaaattc tgagctagcc gttatattgt
    13201 cgcctacata acgttacatt aaggggttac caatggctcg taaatggttg aacttgtttg
    13261 ccggggcggc actctctttc gctgttgctg gcaatacact ggcagatgaa gggaaaatca
    13321 cggtgttcgc cgccgcatca ctgactaacg caatgcagga cattgctacg cagtataaaa
    13381 aagaaaaagg cgtggatgtg gtttcttctt tcgcttcgtc atctactctg gcccgtcaga
    13441 ttgaagcggg agcgcctgcg gatctgttta tctctgccga tcagaaatgg atggattatg
    13501 cggttgataa aaaagcgatc gatacggctt cgcgccagac actgctcggc aatagcctgg
    13561 tcgttgtagc accgaaagcc agcgagcaga aagatttcac catcgacagc aaaaccaact
    13621 ggacttcgct gctgaatggc ggtcgcctcg cggttggcga tccggaacat gtccctgctg
    13681 gcatttatgc gaaagaagca ctgcaaaaac tgggcgcatg ggatacgctc tccccgaaac
    13741 tggctccggc ggaagatgtt cgtggcgcgc tggcgttggt cgaacgcaat gaagcgccgt
    13801 tgggcattgt ttacggttct gacgcagttg ccagcaaagg ggtaaaagtg gttgccacct
    13861 tcccggaaga ttcacataaa aaagtggaat atccggttgc tgttgtggaa gagcataaca
    13921 atgcgacagt gaaagcattt tatgattatt tgaaagggcc gcaggctgcg gaaatcttta
    13981 aacgttacgg atttacaacc aagtaatgat actgaccgat ccagaatggc aggcagtttt
    14041 attaagcctg aaagtttctt ccctggctgt gctgtttagc ctgccgtttg ggatcttttt
    14101 tgcctggtta ctggtgcgtt gcacgtttcc gggcaaagct ctgctcgaca gcgtactgca
    14161 tctaccgctg gtgttaccgc ccgtggtcgt cggttactta ttattagttt cgatgggacg
    14221 gcgcggattt atcggtgaac gtctgtatga ctggtttggt attaccttcg cctttagctg
    14281 gcgcggcgcg gttctcgctg ccgccgtcat gtcgtttccg ctgatggtgc gggcaattcg
    14341 tctggcgctg gaaggggttg atgtcaaact cgaacaggcc gcaagaacac tgggggccgg
    14401 gcgctggcgc gttttcttta ctatcacgtt accgctgacc ttaccgggaa ttattgttgg
    14461 tacggtactg gcttttgctc gttctctcgg tgagtttggt gcaaccatca cctttgtgtc
    14521 gaacattcct ggtgaaacgc gaaccattcc ttctgccatg tataccctga tccagacccc
    14581 cggcggggaa agtggcgcgg cgagactgtg cattatttct attgcgctgg cgatgatctc
    14641 cctgttgatt tcagaatggc tggccagaat cagccgtgaa cgggcggggc gctaatcatg
    14701 ctggaactga atttttccca gaccttgggc aaccattgcc tgaccattaa tgaaacgctg
    14761 cccgccaatg gcatcactgc tatctttggc gtctccggtg ccggaaaaac ttcgctgatt
    14821 aacgccatca gtggactgac gcgcccgcaa aaagggagga ttgtcctcaa tggtcgggta
    14881 ctaaatgatg ccgaaaaagg tatctgcctg acgccagaaa agcgtcgcgt tggctatgtt
    14941 tttcaggatg cgcggctgtt tccgcattac aaagtgcgtg gcaatctgcg ctacggcatg
    15001 gcgaaaagca tggtcgatca gttcgataag ctggtggcgc ttttaggcat tgaaccgttg
    15061 cttgaccgtt taccaggcag cctgtccgga ggcgaaaaac agcgcgtagc gattgggcgg
    15121 gctttgctga cggcaccaga attgctgttg ctggatgaac cgctggcgtc actggatatt
    15181 ccgcgtaaac gcgaactgtt gccttatctg caacgtctga cgcgggaaat caacatcccg
    15241 atgttgtatg tcagccattc gctggatgag atcctccatc tggcagacag agtgatggta
    15301 ctggaaaacg gtcaggtgaa agcctttggc gcactggaag aagtctgggg tagtagcgtg
    15361 atgaatccgt ggctgccgaa agagcaacaa agtagcattc tgaaagtgac ggtacttgag
    15421 catcatccgc attacgcgat gactgcgctg gcgctgggcg atcagcattt gtgggtcaat
    15481 aagctggacg aaccgctgca agctgcgcta cgtatccgca ttcaggcttc cgatgtttct
    15541 ctggttttac aaccgccgca gcaaaccagc attcgtaacg tattgcgggc aaaagttgtt
    15601 aatagttatg acgacaacgg ccaggtggaa gtggaactgg aagtcggagg taaaacgctg
    15661 tgggcgcgta tcagcccgtg ggccagggat gaactggtga tcaaacctgg cctgtggctg
    15721 tacgcgcaaa ttaaaagtgt gtcgataacc gcctgattaa atcaggtggc tataaatgaa
    15781 ctgggcaatg ctgtcggtgg tgttatcacc aatcacaatg ttggcgcgcg cttttaccgc
    15841 gtcatcggcg ttgcccatcg ccacgcctgt accagcggcc tccagcatac tgatatcatt
    15901 aaagttatcg ccgaacgcca cgacgttttc catcgaccag ccttgcgcct caacccattt
    15961 cgtcaaacgt ttacctttgc tgttgccgcc gcgtgcaata tcaacctgat cgtgccagga
    16021 ccattcacac tccagtccca gttcatgttc gacatgctta ccaaaatgct gcaattgcgg
    16081 caggtcatcg tgcgtcaggg cgaacttcca tacggcgtta acttgttgcg ccgtttcagc
    16141 cagagaagcg acttgtgtga aagtcggacg ctgttccggc ggcagggttt gcgcccagtt
    16201 agacgtgcga atgacatgcc cggtcgggtg ctcatagacc attgcatcat ccacatacat
    16261 cagaccgtga atgtggtgtt cattcagcat ctcaatgagt tgcagggctt tattaacggg
    16321 cattgggtcc gcttccagca cggtttttgc atgataatca tacaaatagg tgccattaca
    16381 gcaaatagca ggtgtatcca gcgccagcgc ctgataaaaa ggatgaatag cgacgtgatg
    16441 gcgacctgtg acgatgatta attgatagcc tgcttcgcga gcgcgggcca gggcttctat
    16501 cgatgaagga agcagggtct ttttcggggt caataaggtg ccgtctaagt cgagagcaat
    16561 cacgcgtgtg gtcatggcgt attccagatt aaggttaaga attttcgtct gcgcgaatgg
    16621 tacaccgata ccactatcgg acaaaattct gcgttttaat tcagcattca ccgccaaaag
    16681 cgactaattt tagctgttac agtcagttgc taaatgcaaa ggagcattca tgaagcaaac
    16741 agtttatatc gccagccctg agagccagca aattcacgtc tggaacctga atcatgaagg
    16801 cgcactgacg ctgacacagg ttgtcgatgt gccggggcag gtgcagccga tggtggtcag
    16861 cccggacaaa cgttatctct atgttggtgt tcgccctgag tttcgcgtcc tggcgtatag
    16921 tatcgccccg gacgatggcg cactgacctt tgccgcagag tctgcgctgc cgggtagtcc
    16981 gacgcatatt tccaccgatc accaggggca gtttgtcttt gtaggttctt acaatgcggg
    17041 taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg ggcgtcgtcg atgtggtcga
    17101 ggggctggac ggttgccatt ccgccaatat ctcaccggac aaccgtacgc tgtgggttcc
    17161 ggcattaaag caggatcgca tttgcctgtt tacggtcagc gatgatggtc atctcgtggc
    17221 gcaggaccct gcggaagtga ccaccgttga aggggccggc ccgcgtcata tggtattcca
    17281 tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac agctcagtgg atgtctggga
    17341 actgaaagat ccgcacggta atatcgaatg tgtccagacg ctggatatga tgccggaaaa
    17401 cttctccgac acccgttggg cggctgatat tcatatcacc ccggatggtc gccatttata
    17461 cgcctgcgac cgtaccgcca gcctgattac cgttttcagc gtttcggaag atggcagcgt
    17521 gttgagtaaa gaaggcttcc agccaacgga aacccagccg cgcggcttca atgttgatca
    17581 cagcggcaag tatctgattg ccgccgggca aaaatctcac cacatctcgg tatacgaaat
    17641 tgttggcgag cagggactac tgcatgaaaa aggccgttat gcggtcgggc agggaccaat
    17701 gtgggtggtg gttaacgcac actaaccgct gatttaaccg gcgcagtctc tcctgcgccg
    17761 gtgtattaac ctatctcctg taacgcatgt ctctggcgtt cgacgatatt ggtccacaaa
    17821 ttgtctttat cgtcagtcca caaattaatc agcaaggcaa aaaagcgttc tgctgccgga
    17881 gaaagcacgg catctttgcg gcgaataatc cccaatgtcc gacgtataac gggttccacc
    17941 agtggaatac caataatcga agaatagggc gcatggggca ttgccagacc aggaagcgcc
    18001 gaaatcccca gtcccgcctc taccagtcct aatgacgtcg aaagatgacg cacttcgtaa
    18061 aaccagtcca gcttccaggg cttgtcggct agctgttgtt ctatcaacaa tcggttgccg
    18121 ctggaggaac gtacgccaat cattttgtaa cccactaact cctgccattc tacaagctgc
    18181 tttttggcca atggatggtc acgccgacag gccagcacga atggctcgtt gaccagtggg
    18241 gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta tgccaaagtc tgattcgtta
    18301 cacagcaccg attccatgca attattcgtg ccttgttcca gaatccgcac tttaatattg
    18361 ggatacagct cattaaattt accgattgcc agcggtaaaa aataaaatac tgcggtcgga
    18421 atacacgcta acgtcaccat accacgatga taagcattca tatcacgaat attaaaaagc
    18481 gtttcatcga atttctttat taattcccgc gcctccggga gcaacctttt tcctgctttt
    18541 gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa tatgtaaatc ttcctccatc
    18601 ttttttattc ttcgcgttaa tgcaggttgc gtaatattga gtaatttagc agcattgtta
    18661 aatgaactgg actctgccag tatgacaaat gccttcatac ttgataattc atgcttcatt
    18721 attactccgg aaaatggaag cgacgatttt gggtggctgg tcgttaaaaa ttttaactgc
    18781 atttagccaa cttaaattaa tgaaaaaatg ttattaatcg ttgagctaaa gtcattagag
    18841 atgctttgtc cttaatgtaa ccatatcgca ataagttatg tttttaaatt gagggcatta
    18901 ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac ctcgaggggc gcgttcctgt
    18961 tagcggaaca tttacccgaa gatcaaacgc agcgcgataa aatattgatg gcaattatgg
    19021 gttccggtaa cgatctggaa attgacggta ttggcggcgg taatccactg accagtaaag
    19081 tcgccattat tagccgttcc agcgatccgc gtgctgatgt tgattatctg tttgctcaag
    19141 ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg cggcaatatg ctttctggcg
    19201 ttggggcatt cgccattgaa aatggtttga ttgcagcgac atcaccggtc acccgtgtgc
    19261 gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga tgtgcaaacg ccaaatggtg
    19321 ttgtcgagta cgagggtagt gccagaattg acggcgtacc gggtactgcc gcaccggttg
    19381 cgctcacttt cctgaatgcc gctggaacca aaaccggaaa agttttcccg actgataatc
    19441 agattgatta ttttgacgat gtcccggtga cctgtatcga tatggcgatg ccagtcgtca
    19501 ttattccggc tgaatatctg ggaaaaacag gttatgaatt accggcggaa ctggatgccg
    19561 acaaagcatt attagcccgc attgaatcta tccgtctaca agcgggtaaa gcaatgggct
    19621 taggtgatgt cagtaatatg gttatcccta aacctgtgct tatttctcca gcgcagaaag
    19681 gcggggcaat taatgtgcgt tattttatgc cgcattcttg ccatcgcgcg ctggcgataa
    19741 ccggtgctat tgctatttcc agtagttgtg cattggaagg caccgtcacc cgacaaatcg
    19801 tcccttctgt aggatacggc aatatcaata ttgaacaccc cagtggtgcg ctcgacgttc
    19861 atttaagtaa tgaaggtcag gatgccacga cgttacgcgc atctgttatt cggacgacca
    19921 gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaaa ttcgttgtca agataaggat
    19981 aatcaataaa ggacttctgt atgagtcata cagaaagaac aggattttaa atgaataaga
    20041 aatcgttatg gaagctaatt ctgatattag cgatcccatg tattattggt tttatgccag
    20101 ctccggcagg attaagcgaa ctggcgtggg tgctttttgg tatttacctg gcggccattg
    20161 tggggctggt tatcaagcct ttcccggaac ctgtcgtact gttaattgcc gttgctgcct
    20221 ccatggtggt ggtcggtaac ttatccgacg gtgcgtttaa gaccaccgcc gtattaagcg
    20281 gttactcttc aggtactacc tggctggtgt tctcggcgtt taccttaagc gccgcatttg
    20341 tgaccaccgg tttaggtaaa cgtattgcct atctgctgat tggtaaaatc ggtaacacca
    20401 cgctgggtct gggttacgtt acggtattcc tcgatctggt actggctccg gcaacaccgt
    20461 ctaacaccgc gcgtgcgggc ggtattgtgt taccgatcat caacagcgtg gcggtggctt
    20521 tggggtccga accggaaaaa agtccgcgtc gtgtcggaca ttacctgatg atgtccattt
    20581 acatggtcac caaaaccacc agctatatgt tctttaccgc aatggcgggg aacattctgg
    20641 cgctgaaaat gatcaacgac attctgcacc tgcaaattag ctggggtgga tgggcactgg
    20701 ccgccggatt gccgggcatc attatgctgc tggtcacccc actggtgatt tataccatgt
    20761 atccgccaga aattaagaag gtggataata aaaccatcgc taaagcgggc cttgccgaac
    20821 tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt ctttgtgctg gcgctactgg
    20881 gctggatttt cagtaagtct ctgggggttg atgaatccac tgtggcaatc gttgttatgg
    20941 cgaccatgct gctgctgggt atcgttacct gggaagacgt ggttaaaaat aaaggcggct
    21001 ggaatacctt aatctggtac ggcggtatta tcggattaag ctccttatta tcgaaagtta
    21061 aattcttcga atggttagct gaagtcttta aaaataacct ggcatttgat ggtcacggta
    21121 acgttgcttt cttcgttatt attttcctca gcattatcgt gcgttatttc ttcgcttccg
    21181 gtagtgccta tatcgttgct atgttaccgg tatttgccat gctggcgaac gtctccggcg
    21241 caccgttaat gttaaccgcg ctggcactgt tgttctccaa ctcctatggc ggcatggtta
    21301 ctcactatgg cggcgcggca ggtccggtca tctttggcgt gggttataac gatattaaat
    21361 cctggtggtt ggtcggtgcg gtactgacga tattaacctt cctggtgcat atcaccctcg
    21421 gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct gtaaatattc tcgtcatact
    21481 tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta tgtaagctcc
    21541 tggggattca ctcgcttgtc gccttcctgc aactcgaatt atttagagta tatccattta
    21601 ttatctttct gcgcacttca cggtgcgcag atatctggag catttgatga tcaagttatc
    21661 tgaaaaaggc gtgtttctcg ccagtaataa cgaaataatt gccgaagaac atttcaccgg
    21721 cgaaattaaa aaagaagaag ccaaaaaagg cactattgcc tggtctattc tctcttcgca
    21781 taatacgtcc ggaaatatgg ataaacttaa aattaagttt gattcattag cctctcacga
    21841 tattaccttt gttggtattg tacagaccgc taaagcgtcc ggtatggaac gtttcccgct
    21901 gccgtatgtg ctgaccaact gccataactc actctgcgcc gtcggcggca ctattaacgg
    21961 tgatgaccat gtttttggtt tatcggcggc ccagcgttat ggcggtattt ttgtgcctcc
    22021 gcatattgcg gtcatccatc aatatatgcg tgagatgatg gcaggcggcg gcaaaatgat
    22081 cctcgggtca gacagccaca cccgttacgg tgcattaggg acaatggcag tcggtgaggg
    22141 cggcggtgag ttggtaaaac agctgcttaa tgacacctgg gatatcgact atccgggcgt
    22201 ggttgcggtg catctgaccg gaaaacctgc gccgtatgtg gggccacagg atgtggcgct
    22261 ggctatcatt ggcgcggtgt tcaaaaacgg ttacgtcaaa aacaaagtca tggagttcgt
    22321 tggaccgggc gttagcgcgc tctctaccga tttccgtaac agcgttgacg tgatgaccac
    22381 ggaaaccacc tgtttaagct ccgtctggca aactgatgaa gaagtgcata actggctggc
    22441 gctgcacggt cgcggccagg attactgcca gcttaaccct caaccgatgg cgtactacga
    22501 tggctgcatc agcgttgatt taagcgccat caaaccaatg attgcgctgc cgttccaccc
    22561 gagcaacgtg tatgaaatcg acacactgaa ccagaacctg accgacattc tgcgtgagat
    22621 tgaaattgag tccgaacgcg tggcgcacgg taaagccaaa ctctcgctgc tggataaagt
    22681 ggaaaatggt cgcctgaaag tgcagcaggg gattatcgcg ggctgttctg gcggtaacta
    22741 cgaaaacgtc atcgcggcgg cgaatgcact gcgcggtcaa tcctgtggca atgacacctt
    22801 ctcgctggca gtttacccgt catcacagcc ggtgtttatg gatctcgcca aaaaaggtgt
    22861 ggtagcagat ttgattggcg caggcgcaat catcagaacc gcgttctgcg gcccatgctt
    22921 tggcgcgggc gatacgccaa tcaacaacgg tttgagtatt cgccacacca cgcgtaactt
    22981 cccgaaccgc gaaggctcta agccagctaa tgggcagatg tcagcggtgg cgttgatgga
    23041 cgctcgttct atcgctgcga ctgcggcaaa cggtggctat ttaacctctg ccagcgaact
    23101 tgattgctgg gacaacgtgc cggagtacgc cttcgatgta acgccgtata aaaaccgtgt
    23161 ttatcagggc tttgtgaaag gggcaactca gcaaccgctg atttacggac cgaacattaa
    23221 agactggccg gaattgggtg cgctgactga caatatcgtc ctgaaagtgt gctcgaagat
    23281 cctcgacgaa gtgaccacca ccgacgaact gattccttcc ggtgaaacct cttcttatcg
    23341 ttcaaatccg attggtctgg cggagtttac cctgtcacgc cgcgatcccg gttatgttgg
    23401 cagaagtaaa gcgactgctg agctggaaaa tcagcgtctg gcggggaatg tcagcgagct
    23461 gacagaggtg tttgcgcgca ttaagcagat tgctggtcag gagcatattg atccgctgca
    23521 aactgaaatt ggcagcatgg tatatgcggt gaaaccaggc gatggttctg cgcgtgaaca
    23581 ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat attgccgaag agtacgcgac
    23641 taaacgctac cgttctaacg tcatcaactg ggggatgtta ccgctgcaga tggcggaagt
    23701 gccaaccttt gaagtggggg attacattta catccctggc attaaagcgg cgctggataa
    23761 tccgggtacg acgtttaaag gttatgtgat ccatgaagat gcgccggtaa cggaaattac
    23821 gctctatatg gaaagtctga ctgctgaaga gcgcgagatt atcaaggcgg gtagtttgat
    23881 taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc
    23941 acttattgtc ggatgcgatg cttgcgcatc ttatccgacc tacgaatcgc atcgaatctg
    24001 taggccagat aaggcatttt cgcagcatcc ggcacttatt gtcggatgcg atgcttgcgc
    24061 atcttatccg acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat
    24121 ccggcaaata gttaattgct cttacttctt cgcctctgca accactttac tacccacgcc
    24181 gcggttattg tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc
    24241 gtcgttatca tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc
    24301 ggcccacggt ttagccgtgt taaaaccttc gttgatggcg ctatcccgga tcaccacctg
    24361 accgttggta ttggcatcaa catccagcga gcggcccagt tgcgccacac catcaccgaa
    24421 agcattgaaa cggctgttta cggcgaggaa accgtagtaa atgttggaca gcgtagccgg
    24481 tgcaaacaca tacgcttctt gctgagtacg tgagttcacc acgcggaatt cggtgttatc
    24541 gaacaccact gcgccgcgac cagaaacgat atccacatcc ccttcaatgt agctgttggt
    24601 caccagcgta cgcggctgac gattcgtttc cagacggttc tgcacaccgc tgttggtgac
    24661 aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta atctgtacct ggtcaccatc
    24721 agtacgcagt gccaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc
    24781 gatggtcaga ttttgcagtt gcaggccatt gttttgtgac cagaagaccg cagagcagag
    24841 aacaccgata ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt
    24901 acctggcata tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc
    24961 accatcaagg gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt
    25021 aattccaccc ggagcggcag ggacatatac cgttccctga tactcaccag gcatcacggc
    25081 aatatactgg cgcttgttgg tacgcttgat aattgccgca tctaccgccg cctgaatcgt
    25141 ggtatgcgtt acaccttgag tgcccgccgg gccgacaaca aagtcaggtt gcgcaggcag
    25201 ggtaatcggg gaaggattcc acgctgctgc acctggtgtc agggatgcaa aatagtgttg
    25261 agcatcgaaa ttctgcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc
    25321 ggtttgatca gaaggacgtt gatcgggcgg ggttgagcta caggcggtca gcgtcacgcc
    25381 aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct
    25441 atgaaataga aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa
    25501 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
    25561 tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg
    25621 gtgtccgact tatgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag
    25681 agtcttgcag acaaactgcg caactcgtga aaggtcggcg gatccccttc gaaggaaaga
    25741 cctgatgctt ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca
    25801 cgatgagtag atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt
    25861 tccttcattg atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg
    25921 cctgttttgc tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg
    25981 cataaatcac caactcgctg cccggtaaca acagccagct ccattgcaag tcttagccaa
    26041 catggtgatg attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat
    26101 ctccttacct ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct
    26161 tcagctattg cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgacgcc
    26221 gccttgccct cgtctatgta tccactgagc attgccgcaa tttcttttgt ggtgatgtct
    26281 tcaagtggag catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg
    26341 agagtcttct gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa
    26401 tgtaacgtaa cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct
    26461 gaaaataact caat
//
LOCUS       DAZFDP010000054        26391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFDP010000054 DAZFDP010000000
VERSION     DAZFDP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHJ1G_002329"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHJ1G_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4764176.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHJ1G_002330"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHJ1G_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4764177.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHJ1G_002331"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHJ1G_002331"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4764178.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHJ1G_002332"
     CDS             complement(1154..1621)
                     /locus_tag="ACHJ1G_002332"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4764179.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHJ1G_002333"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHJ1G_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4764180.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHJ1G_002334"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHJ1G_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4764181.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHJ1G_002335"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHJ1G_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4764182.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHJ1G_002336"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHJ1G_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4764183.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHJ1G_002337"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHJ1G_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4764184.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHJ1G_002338"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHJ1G_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4764185.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHJ1G_002339"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHJ1G_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4764186.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHJ1G_002340"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHJ1G_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4764187.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHJ1G_002341"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHJ1G_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4764188.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHJ1G_002342"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHJ1G_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4764189.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHJ1G_002343"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHJ1G_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4764190.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHJ1G_002344"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHJ1G_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4764191.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHJ1G_002345"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHJ1G_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4764192.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHJ1G_002346"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHJ1G_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4764193.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHJ1G_002347"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHJ1G_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4764194.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHJ1G_002348"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHJ1G_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4764195.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEGFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHJ1G_002349"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHJ1G_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4764196.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHJ1G_002350"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHJ1G_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4764197.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHJ1G_002351"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHJ1G_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4764198.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHJ1G_002352"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHJ1G_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4764199.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHJ1G_002353"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHJ1G_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4764200.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHJ1G_002354"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHJ1G_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4764201.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHJ1G_002355"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHJ1G_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4764202.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHJ1G_002356"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHJ1G_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4764203.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHJ1G_002357"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHJ1G_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4764204.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHJ1G_002358"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHJ1G_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4764205.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHJ1G_002359"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHJ1G_002359"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4764206.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHJ1G_002360"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHJ1G_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4764207.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHJ1G_002361"
     CDS             15761..16129
                     /locus_tag="ACHJ1G_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4764208.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHJ1G_002362"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHJ1G_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4764209.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHJ1G_002363"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHJ1G_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4764210.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHJ1G_002364"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHJ1G_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4764211.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHJ1G_002365"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHJ1G_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4764212.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHJ1G_002366"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHJ1G_002366"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4764213.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHJ1G_002367"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHJ1G_002367"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4764214.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHJ1G_002368"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHJ1G_002368"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4764215.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHJ1G_002369"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHJ1G_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4764216.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHJ1G_002370"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHJ1G_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4764217.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHJ1G_002371"
     CDS             23308..23850
                     /locus_tag="ACHJ1G_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4764218.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHJ1G_002372"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHJ1G_002372"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4764219.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHJ1G_002373"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHJ1G_002373"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4764220.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHJ1G_002374"
     CDS             25247..25504
                     /locus_tag="ACHJ1G_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4764221.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHJ1G_002375"
     CDS             complement(25480..26034)
                     /locus_tag="ACHJ1G_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4764222.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6775 a         6274 c         6843 g         6499 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttctgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaaggttt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtagttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat
    15961 tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca
    17101 attaataagc ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttagcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt
    19741 gcgacagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 actgctttag gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcaaatt caggtgctta
    23101 accttgaaac ccgtgaaatg gtgatagagc gagtgctcgc gctggatacc gcagagttcg
    23161 acctggaaga cctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ttgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat
    23761 tatccactac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gttgggtgcg atcctgatag cgaaacagca gtgatgagac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
    24601 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc
    24721 tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtcctggttt acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc
    24841 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa gagaaaaaat
    26281 cctgaaatta agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt
    26341 gagctgaaag ccgcgtcgca actgctcttt aacaatttat cagacaatct g
//
LOCUS       DAZFDP010000055        26214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFDP010000055 DAZFDP010000000
VERSION     DAZFDP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHJ1G_002376"
     CDS             complement(<1..434)
                     /locus_tag="ACHJ1G_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4764223.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGG"
     gene            complement(594..806)
                     /gene="rpmE"
                     /locus_tag="ACHJ1G_002377"
     CDS             complement(594..806)
                     /gene="rpmE"
                     /locus_tag="ACHJ1G_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4764224.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1009..3207
                     /gene="priA"
                     /locus_tag="ACHJ1G_002378"
     CDS             1009..3207
                     /gene="priA"
                     /locus_tag="ACHJ1G_002378"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4764225.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3363..4388
                     /gene="cytR"
                     /locus_tag="ACHJ1G_002379"
     CDS             3363..4388
                     /gene="cytR"
                     /locus_tag="ACHJ1G_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4764226.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4480..5439
                     /gene="ftsN"
                     /locus_tag="ACHJ1G_002380"
     CDS             4480..5439
                     /gene="ftsN"
                     /locus_tag="ACHJ1G_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4764227.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQPPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5532..6062
                     /gene="hslV"
                     /locus_tag="ACHJ1G_002381"
     CDS             5532..6062
                     /gene="hslV"
                     /locus_tag="ACHJ1G_002381"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4764228.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6072..7403
                     /gene="hslU"
                     /locus_tag="ACHJ1G_002382"
     CDS             6072..7403
                     /gene="hslU"
                     /locus_tag="ACHJ1G_002382"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4764229.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7470..8396
                     /gene="menA"
                     /locus_tag="ACHJ1G_002383"
     CDS             7470..8396
                     /gene="menA"
                     /locus_tag="ACHJ1G_002383"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4764230.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8489..8974
                     /gene="rraA"
                     /locus_tag="ACHJ1G_002384"
     CDS             8489..8974
                     /gene="rraA"
                     /locus_tag="ACHJ1G_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4764231.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9059..9304)
                     /gene="zapB"
                     /locus_tag="ACHJ1G_002385"
     CDS             complement(9059..9304)
                     /gene="zapB"
                     /locus_tag="ACHJ1G_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4764232.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9729..10574
                     /gene="glpF"
                     /locus_tag="ACHJ1G_002386"
     CDS             9729..10574
                     /gene="glpF"
                     /locus_tag="ACHJ1G_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4764233.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10597..12105
                     /gene="glpK"
                     /locus_tag="ACHJ1G_002387"
     CDS             10597..12105
                     /gene="glpK"
                     /locus_tag="ACHJ1G_002387"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4764234.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12240..13250
                     /gene="glpX"
                     /locus_tag="ACHJ1G_002388"
                     /pseudo
     CDS             12240..13250
                     /gene="glpX"
                     /locus_tag="ACHJ1G_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            13347..14093
                     /gene="fpr"
                     /locus_tag="ACHJ1G_002389"
     CDS             13347..14093
                     /gene="fpr"
                     /locus_tag="ACHJ1G_002389"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4764235.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14098..14526)
                     /gene="uspD"
                     /locus_tag="ACHJ1G_002390"
     CDS             complement(14098..14526)
                     /gene="uspD"
                     /locus_tag="ACHJ1G_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4764236.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14553..14852)
                     /gene="yiiS"
                     /locus_tag="ACHJ1G_002391"
     CDS             complement(14553..14852)
                     /gene="yiiS"
                     /locus_tag="ACHJ1G_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4764237.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15064..15504)
                     /gene="yiiR"
                     /locus_tag="ACHJ1G_002392"
     CDS             complement(15064..15504)
                     /gene="yiiR"
                     /locus_tag="ACHJ1G_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4764238.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15605..16204
                     /gene="yiiQ"
                     /locus_tag="ACHJ1G_002393"
     CDS             15605..16204
                     /gene="yiiQ"
                     /locus_tag="ACHJ1G_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4764239.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16312..17079
                     /gene="tpiA"
                     /locus_tag="ACHJ1G_002394"
     CDS             16312..17079
                     /gene="tpiA"
                     /locus_tag="ACHJ1G_002394"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4764240.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17134..17889)
                     /gene="cdh"
                     /locus_tag="ACHJ1G_002395"
     CDS             complement(17134..17889)
                     /gene="cdh"
                     /locus_tag="ACHJ1G_002395"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4764241.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17996..18985)
                     /gene="sbp"
                     /locus_tag="ACHJ1G_002396"
     CDS             complement(17996..18985)
                     /gene="sbp"
                     /locus_tag="ACHJ1G_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4764242.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19304..20266)
                     /gene="pfkA"
                     /locus_tag="ACHJ1G_002397"
     CDS             complement(19304..20266)
                     /gene="pfkA"
                     /locus_tag="ACHJ1G_002397"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4764243.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20447..21349)
                     /gene="fieF"
                     /locus_tag="ACHJ1G_002398"
     CDS             complement(20447..21349)
                     /gene="fieF"
                     /locus_tag="ACHJ1G_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4764244.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21498..21998)
                     /gene="cpxP"
                     /locus_tag="ACHJ1G_002399"
     CDS             complement(21498..21998)
                     /gene="cpxP"
                     /locus_tag="ACHJ1G_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4764245.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22148..22846
                     /gene="cpxR"
                     /locus_tag="ACHJ1G_002400"
     CDS             22148..22846
                     /gene="cpxR"
                     /locus_tag="ACHJ1G_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4764246.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22843..24216
                     /gene="cpxA"
                     /locus_tag="ACHJ1G_002401"
     CDS             22843..24216
                     /gene="cpxA"
                     /locus_tag="ACHJ1G_002401"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4764247.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24358..25032)
                     /gene="yiiM"
                     /locus_tag="ACHJ1G_002402"
     CDS             complement(24358..25032)
                     /gene="yiiM"
                     /locus_tag="ACHJ1G_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4764248.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25181..26164)
                     /gene="kdgT"
                     /locus_tag="ACHJ1G_002403"
     CDS             complement(25181..26164)
                     /gene="kdgT"
                     /locus_tag="ACHJ1G_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4764249.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         6436 a         6865 c         6775 g         6138 t
ORIGIN      
        1 ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg
       61 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      121 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      181 gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg gccgggcagg
      241 gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca gcggattgac cggatggccg
      301 gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc aatgcgcacc
      361 ccggctgaac cctggacaat gctactgcca tactgcgtca tgtcgccctg acgcgctgcc
      421 ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc ctagcgccag
      481 tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc
      541 aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
      601 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
      661 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
      721 ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg
      781 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
      841 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
      901 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
      961 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgtagcc
     1021 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
     1081 acggttaaag ctgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg
     1141 attgtggtat cagttagcga tgccagcgaa ctgccgctca atgagctaaa agcggtagtc
     1201 gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcggttact gctctgggcg
     1261 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg
     1321 cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggt
     1381 caggcggtgg atcttaacag cctgaaacgt tcgccaaagc aacaacaggc gctggcggcg
     1441 ttacggcaag gcaaaatctg gcgcgaccag gtcgcggaac ttgaatttaa tgatgccgcg
     1501 ttgcaggcgc tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
     1561 gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaagcc
     1621 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
     1681 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
     1741 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
     1801 tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt ccggcctgaa cgacagcgag
     1861 cggctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc
     1921 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgatga agagcacgac
     1981 agctcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt
     2041 gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgtta
     2101 tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cccgtcgggc agggaatgcg
     2161 cgtccggcaa ttcaacatgt gctggattta aaaggtcaga aggtgcaggc aggtctggct
     2221 ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt
     2281 cttaatcgtc ggggttttgc gcctgcgctg ctgtgccacg actgcggctg gatagccgaa
     2341 tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac
     2401 cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg
     2461 gtccccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgat gttccccggc
     2521 gtgcccattt cacgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa
     2581 ctggcggaag tgcatcgcgg cggcgcgagg atcttgattg gcacgcaaat gctggcgaaa
     2641 ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt
     2701 tctgccgatt ttcgctcggc agaacgtttc gcccagcttt acacccaggt cgccggtcgt
     2761 gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct
     2821 ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc gctggctgag
     2881 cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc ggaagatcat
     2941 aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct ttccagcccg
     3001 ctggcagacg acaaactgtg ggttctcggc ccggttccgg ctctggcacc taagcgtggt
     3061 ggccgctggc gctggcagat cttgttgcag cacccttccc gcgtgcgctt gcaacacatc
     3121 attagtggta cgctggcgct tattaataca ataccggatt cccgcaaggt gaaatgggtg
     3181 ttggatgtcg atcccatcga aggctaatgc gcaaacgttg cgaggcggat cgaaaaattc
     3241 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
     3301 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
     3361 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
     3421 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
     3481 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
     3541 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc
     3601 ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg
     3661 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
     3721 tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca
     3781 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca ccggaactgg
     3841 agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta aattatctat
     3901 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc
     3961 actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc
     4021 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg cagcagctgc
     4081 ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg
     4141 cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt
     4201 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc
     4261 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
     4321 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
     4381 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
     4441 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
     4501 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
     4561 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
     4621 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
     4681 cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa
     4741 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa
     4801 gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct
     4861 gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca gacgccggaa
     4921 cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga gcagcaacgc
     4981 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcgcaa
     5041 gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac
     5101 caggatctgc tgcaaccccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
     5161 ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
     5221 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
     5281 ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc
     5341 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg
     5401 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
     5461 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
     5521 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
     5581 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
     5641 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
     5701 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg
     5761 ttgagctagc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
     5821 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
     5881 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgtgcac
     5941 tgttagaaaa tactgaactg agtgctcgtg aaattgctga aaaggcgttg gatattgcag
     6001 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
     6061 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
     6121 tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc
     6181 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
     6241 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
     6301 cgttcatcaa agttgaagcg accaaattca ccgaagtagg ctacgtcggt aaggaagtgg
     6361 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
     6421 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc
     6481 cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc
     6541 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc
     6601 ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca
     6661 gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga
     6721 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
     6781 aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
     6841 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
     6901 ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg
     6961 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
     7021 acctaatccc ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag gcactgacca
     7081 ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
     7141 cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt aagcgcatcg
     7201 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca
     7261 ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
     7321 atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
     7381 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
     7441 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
     7501 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
     7561 ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactaa
     7621 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa
     7681 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
     7741 cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggt
     7801 tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg
     7861 gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata
     7921 tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagtgtc atggggagct
     7981 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc gcatgcggcc
     8041 tgctggcaac ggcagtactg aatattaata acctgcgtga tatcaatagc gaccgcgaaa
     8101 atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct
     8161 gcctgctgat gggctcgctg gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc
     8221 tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga
     8281 tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt
     8341 tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga
     8401 caaatatcaa ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa
     8461 attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat
     8521 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
     8581 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
     8641 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg
     8701 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt
     8761 catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
     8821 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
     8881 caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
     8941 tattctttct gaagatccgc tggatattga atgatagaaa aggcaccgca aggtgccttt
     9001 tttctgcgtt acctgttggc ctacacagta aagaaattac gcggaagatg aagcgtaatc
     9061 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt
     9121 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
     9181 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
     9241 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
     9301 tcatacctct cctgaattgc aaggcgttga tggataaaaa tcctcgtccc gattaccggt
     9361 gacgccttaa taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc
     9421 gctgtccagc ggcacgcctt gcagattacg gtttgccaca cttttcatcc ttctcctggt
     9481 gacataatcc acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac
     9541 atgcatcatg tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacgaagc
     9601 acacacattt taagttcgat atttctcgct tttgctcgtt aacgataagt ttacagcatg
     9661 cctacaagca tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg
     9721 atccgattat gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta
     9781 ccgggttgtt gattttcttc ggtgtgggtt gcgttgcagc actaaaagtc gctggtgcgt
     9841 cttttggtca gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc
     9901 tgaccgcagg ggtttccggc gcgcatctta atcccgctgt taccattgca ttgtggctgt
     9961 ttgcctgttt cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt
    10021 tctgtgctgc ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga
    10081 ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt
    10141 accctaatcc tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta
    10201 ttctgatggg gctgatcctg gcgttaacgg atgatggcaa cggtgtacca cgcggccctt
    10261 tggctccctt gctgattggt ctactgattg cggtcattgg cgcatctatg ggcccattga
    10321 caggttttgc catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tggctggcgg
    10381 gctggggcaa tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt
    10441 tcggccctat cgttggcgcg attgtaggtg catttgccta ccgcaaactg attggtcgcc
    10501 atttgccttg cgatatctgt gttgtggaag aaaaggaaac cacaactcct tcagaacaaa
    10561 aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt
    10621 gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc
    10681 attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggttg ggtagaacac
    10741 gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc
    10801 gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt
    10861 gtctgggaaa aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt
    10921 accgcagaaa tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat
    10981 accggtctgg tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat
    11041 gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg
    11101 tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct
    11161 cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct ggaagtgctg
    11221 gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact
    11281 aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag
    11341 gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact
    11401 ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg
    11461 accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg tgcggtgttt
    11521 atggcaggcg catccattca gtggctgcgc gatgagatga agttgattaa cgacgcctac
    11581 gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca
    11641 tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat tttcggtctg
    11701 actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag
    11761 acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca cgccctgcgc
    11821 gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga tattctcggc
    11881 acccgcgttg agcgcccgga agtgcgcgaa gtcaccgcat tgggtgcggc ctatctcgca
    11941 ggcctggcgg ttggcttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgag
    12001 cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa
    12061 aaagcggtta aacgcgcgat ggcgtgggaa gaacacgacg aataatgtaa atgccgaatg
    12121 aagcgtttat gccgcatccg gtagtcccga aacgtgcggg ggcaaccccg cacacatcaa
    12181 taatccctcc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    12241 tgagacgaga acttgccatc gaattttccc gcgtcaccga atcagcggcg ctggctggct
    12301 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    12361 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    12421 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    12481 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    12541 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    12601 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    12661 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    12721 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    12781 tatttgctat tccggatggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    12841 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtagtttct gcggcggtga
    12901 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    12961 acaactaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    13021 tcgaagccgg taaagtattg cgcctgggcg atatggcgcg cagcgataac gtcatcttct
    13081 ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat tagccgcaaa ggcaatatcg
    13141 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    13201 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca catcctctga ttgatttgat
    13261 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    13321 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    13381 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    13441 ccgccgggca attcaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    13501 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    13561 atggcaaatt aagcccacga ttggcggcac tgaaaccagg cgatgaagtg caggtggtta
    13621 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    13681 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    13741 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    13801 tgccactgat gcaagaactg gaaaaacgct acgaagggaa actgcgcatt cagacggtgg
    13861 tcagtcggga aacggcagcg gggtcgctca ccgggcggat accggcgtta attgaaagtg
    13921 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    13981 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    14041 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    14101 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    14161 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    14221 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    14281 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    14341 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    14401 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagagc
    14461 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    14521 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    14581 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    14641 tccagatcct taatcttgta ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    14701 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    14761 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    14821 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    14881 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    14941 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    15001 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    15061 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    15121 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    15181 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgcccagtt
    15241 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    15301 atcatgcaag cgcttaacag ttactgccgc tgttggccaa agcaagcaca caaggcaaaa
    15361 cgccgcggtc tgaatatcga gtaaattttt acccgccagt gagaaaagca ccagcatgcc
    15421 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    15481 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    15541 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    15601 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    15661 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    15721 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcctag
    15781 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    15841 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    15901 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    15961 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    16021 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    16081 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    16141 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    16201 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    16261 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    16321 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    16381 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    16441 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    16501 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    16561 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    16621 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg cctgactccg
    16681 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gcaaaactga agaagtttgc
    16741 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    16801 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    16861 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    16921 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    16981 cagccagata tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    17041 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    17101 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    17161 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    17221 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    17281 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    17341 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    17401 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    17461 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    17521 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    17581 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    17641 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    17701 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    17761 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    17821 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    17881 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    17941 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctgg ggaaatcagc
    18001 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    18061 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    18121 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    18181 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    18241 ccactttatc gaccaccgac acggttggct ctgcgaggat agactcactc ggcgtgacga
    18301 tttcgaattt atctttcccc agttcattcg ctaccagcag agcttcgttt tcccaggcga
    18361 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    18421 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    18481 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    18541 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    18601 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    18661 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    18721 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    18781 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    18841 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    18901 atgaaacgtt aagaagctga atatcctttg ccataacgtt ggttgccgcc agcaaaaatg
    18961 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    19021 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    19081 gtatatgttg ggggttaaaa aggcggaaga aatccgcctc atattgctga caaagtgcgc
    19141 tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaagtt tgcaaattca
    19201 ataaattgca gaattcatgt aggcctgata agcgaagcgc atcaggcatt tttgcttctg
    19261 tcatcggttt cagggtaaag gaatctgcct ttttccgaaa tcattaatac agttttttcg
    19321 cgcagtccag ccagtcacct ttgaacggac gcttcatgtt ttcgatagcg tcgatgatgt
    19381 cgtggtgaac cagctgttcg ttctggatac ctacacaacg accgccgtaa cctgccagca
    19441 gcagatcgat agcgtaagcg cccatacggg aagccagaat acggtcgtaa ggcaccggag
    19501 aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt ttcacgaccg gtttctttct
    19561 cgatgaaatg cgccagttcg tcaacatcac acatatgttc ggtaatcgcc acgatcgcgt
    19621 gttttttacc tttcgcgata cccgctttga tttcgtttac caggtcttca cggctgaatt
    19681 caacttccgg aaccacaacg aattcacagc caccggcaat ggccgcagcc aacgtcagat
    19741 cgccacaata acggcccatc acttccacca cggaaatacg ctggtgagaa gaagaggtgt
    19801 cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc agtgaagaaa ccgatagtgt
    19861 agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag accgatgcac gggaagccca
    19921 tttcggtcag acgcattgca cccatgtagg aaccgtcacc gccgataacc accagcgcgt
    19981 cgatcccacg ttttttcagg ttttcgatag ccacggcgcg gatgttctcg tcgcggaatt
    20041 ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt gatcatgtca gaaacgctgt
    20101 aacggtctag ctgtaccata cggtcttcat acagacccag atagccgtca taaatgccca
    20161 ttacttccag accttctgtc agcgcagaac gaacaacccc gcgaattgcg gcgttcatgc
    20221 ctggcgcatc accgccgctt gtcaacacac cgattttctt aatcatgact acctctgaac
    20281 tttggaatgc aaaataaaat ctgttgccgg aagtcttctt gcacatcgaa gtgatccaac
    20341 gaatgtgcaa atagtataac aatcacttcc tgctgaattg attcaggtca ggccaaatgg
    20401 cggtatttta tacacaaaat gcgggtctgg ctctctttta tactgattat gaaagcatag
    20461 accgtttacc ctccctgggt acgacggaac aggggtcctg atggataatt acatccgatc
    20521 ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc caccatatgt gcctgaacca
    20581 aaggcagaga gtcttccatt tccaaatgaa tctgaataaa gcgggtcggc cctgactgcc
    20641 gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga agtcacgata tcaataattt
    20701 cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga ctgtaccgcc tcatatccca
    20761 tgcgtaacgc gctatataaa atatagatgc cgattcccaa tgcaaacaga gcatcggcgc
    20821 gatgccagcc gtaccaggac aaccccagcg ccagcagaat tgcgccgttc atcataacat
    20881 cagactggta atgtagcata tcagcccgca ccgcctggct ttgcgtccgg cgcaccaccc
    20941 aacgctgaaa cgagacaagg ataatcgtac aaattagcgc cacaattgtc acgataaccc
    21001 cgacgcctgg atctgtcatc ggtgttggag atatcagatg ttgaataccc gtcaaaaaca
    21061 ggaatagtgc cgaaccggag ataaacatac tttgcgccag cgccgcgagg gactctgctt
    21121 taccgtgacc aaacgagtga ttatcgtcgg caggttgcag ggaatatcgc accaccagta
    21181 aattcgtcaa cgacgcgccg atatccacca gcgaatccac cagcgcggcg agaatactca
    21241 ccgacccggt ataccaccat gcaaaaattt taatcagcaa tagcaacgaa gccatcgccg
    21301 tcgcagcaat cgccgcccga ctgaccagcc gtccataaga ttgattcata aatactcccg
    21361 ctatcaactg acgctagtat aacggaagca aatcatctgc aatgcattaa gcagcaggca
    21421 aattgaggat aaaaaaaacc cccacatcat gtgggggaag acagggatgg tgtctatggc
    21481 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    21541 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    21601 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    21661 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    21721 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    21781 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    21841 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    21901 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    21961 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    22021 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    22081 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    22141 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    22201 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    22261 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    22321 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    22381 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    22441 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    22501 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    22561 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    22621 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    22681 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    22741 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    22801 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    22861 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    22921 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    22981 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    23041 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    23101 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    23161 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    23221 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    23281 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    23341 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    23401 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    23461 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    23521 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    23581 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    23641 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    23701 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    23761 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    23821 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    23881 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    23941 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    24001 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    24061 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    24121 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    24181 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    24241 ggcgtttacg ccgcatccgg catttgagca ggataaggcg tttacgccgc atccggcatt
    24301 tgagcaggat gcctgatgcg acgctgatag cgtcttatca ggcctacact ccgggggtta
    24361 ttttccccac aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat
    24421 cgtccgcgtc cagcttttcg ataacccggc ggcggagagt aaacggtgat actgatcgtc
    24481 atcaaacggc atatgccatg cgatggcggc agcctcctgc acggtcacat cgctgacacg
    24541 ggaaaccaac tccagcggcg cgtccgcaga cactttccct ggcgctatca cgctatacaa
    24601 ccaccccacc ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat caaaatggta
    24661 attgagttta tagcaaggcg agcgcggctg gctgacctga attaatgcct ctccccagcg
    24721 gaaaatatcg cccatatgaa cattactttc cgtgagaccg tcggttgaga gattttcgcc
    24781 aaacgcaggc gcaacaaaca actccgcctg ttccggaaat tcccgcaccc agtagagata
    24841 gtgctcacga ggataatgac acagcgctct gtctggcccg ccgtgaacct ttttctccgc
    24901 ctgctcgtca ccttccagcc ccagctccgt cagcatcaac tcaccatcaa cctggatttt
    24961 ggcaattgcg ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt atacatcaac
    25021 cggatatcgc atctgttccc tctccacgag aaatttcccc attagaccat atttatttta
    25081 tgtcaggcgt gctgttttcg cggtaagtat ccaggcagga actcaatagc aaaccggacg
    25141 aacgttagtt catccggctg tggggattaa ggaagcaggt ttatttcacc gtacctaaaa
    25201 tttcgatttt cgctgctctg gctttgactt tacgtgacca gatagaggta agaattggca
    25261 ccagaatcga agtcacaatg accgccgtcg ctaccagcga agttgctgcc ggagccatcg
    25321 gtttaaacgc aggcaccatt tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg
    25381 aactggaagc ggcaattccg gcagtgccat cgccaccgcc aatcaattta tcggcgataa
    25441 tcaacggaat accggtcacg ataattactg ccacacccaa caggatcccc agcaaaccag
    25501 tctgggcaat cacagtcaaa tcaatggtat tgcccagcgc gaaggcaaag aatggaatca
    25561 gcgtttgcac cgctttgctg aaaaattctc gtaattcagg gtcaaggttc cccagggcaa
    25621 agcccaccag gaacggtaat actgcgccga cgaaaacatg cggttcaaac gaggcaatcc
    25681 ctgcagtgcc cagaataatc atcgtcatga gcggcccgga ctccaacgac atcaacacaa
    25741 atgccccagc ttcttctttt gtgccgtact gctgcatgat ggaagcgtaa agtccgccat
    25801 tggtcatatc catcgccgcc accagcgcca gcgttgaaag tccggcaaag aatccaactt
    25861 caacaccatg ttccggaata atgcgcgagg caattgccgc aaccacccac gcgacggcaa
    25921 tttttgttac caccagcgta ccggatttac gcaataccgt tcccgtcgcg cttaatttta
    25981 ttgacgcccc catgcaaaaa aaccacaccg ccagaatggg caccgtaccg gtaatcatcc
    26041 cgttggtgaa tgatccaaaa tatttccccg cccccggcga gaaggtgtgg cacagtgcgc
    26101 caaggaatag cgggacgagc atcatccccc ccgggatttt ctcaatcgag cgttttatct
    26161 gcatttccat cacctgtcgg catattttta agggctgatg cgtcattcct caat
//
LOCUS       DAZFDP010000056        25605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFDP010000056 DAZFDP010000000
VERSION     DAZFDP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..1490
                     /locus_tag="ACHJ1G_002404"
     CDS             90..1490
                     /locus_tag="ACHJ1G_002404"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4764250.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1646..3181
                     /gene="gadC"
                     /locus_tag="ACHJ1G_002405"
     CDS             1646..3181
                     /gene="gadC"
                     /locus_tag="ACHJ1G_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4764251.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3312..4631
                     /locus_tag="ACHJ1G_002406"
     CDS             3312..4631
                     /locus_tag="ACHJ1G_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4764252.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5008..6390
                     /gene="dosC"
                     /locus_tag="ACHJ1G_002407"
     CDS             5008..6390
                     /gene="dosC"
                     /locus_tag="ACHJ1G_002407"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4764253.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6415..8814
                     /gene="dosP"
                     /locus_tag="ACHJ1G_002408"
                     /gene_synonym="pdeO"
     CDS             6415..8814
                     /gene="dosP"
                     /locus_tag="ACHJ1G_002408"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO4764254.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            9072..9653
                     /gene="ddpX"
                     /locus_tag="ACHJ1G_002409"
     CDS             9072..9653
                     /gene="ddpX"
                     /locus_tag="ACHJ1G_002409"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4764255.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            9667..11217
                     /locus_tag="ACHJ1G_002410"
     CDS             9667..11217
                     /locus_tag="ACHJ1G_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4764256.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            11219..12241
                     /locus_tag="ACHJ1G_002411"
     CDS             11219..12241
                     /locus_tag="ACHJ1G_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4764257.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            12238..13134
                     /gene="ddpC"
                     /locus_tag="ACHJ1G_002412"
     CDS             12238..13134
                     /gene="ddpC"
                     /locus_tag="ACHJ1G_002412"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4764258.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            13131..14117
                     /locus_tag="ACHJ1G_002413"
     CDS             13131..14117
                     /locus_tag="ACHJ1G_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4764259.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            14110..15036
                     /locus_tag="ACHJ1G_002414"
     CDS             14110..15036
                     /locus_tag="ACHJ1G_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4764260.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(15092..15523)
                     /gene="osmC"
                     /locus_tag="ACHJ1G_002415"
     CDS             complement(15092..15523)
                     /gene="osmC"
                     /locus_tag="ACHJ1G_002415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4764261.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15869..16084
                     /gene="bdm"
                     /locus_tag="ACHJ1G_002416"
     CDS             15869..16084
                     /gene="bdm"
                     /locus_tag="ACHJ1G_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4764262.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            16186..16323
                     /gene="sra"
                     /locus_tag="ACHJ1G_002417"
     CDS             16186..16323
                     /gene="sra"
                     /locus_tag="ACHJ1G_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4764263.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            16480..18177
                     /gene="maeA"
                     /locus_tag="ACHJ1G_002418"
     CDS             16480..18177
                     /gene="maeA"
                     /locus_tag="ACHJ1G_002418"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4764264.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            18311..19321
                     /gene="adhP"
                     /locus_tag="ACHJ1G_002419"
     CDS             18311..19321
                     /gene="adhP"
                     /locus_tag="ACHJ1G_002419"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4764265.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            19468..19752
                     /locus_tag="ACHJ1G_002420"
     CDS             19468..19752
                     /locus_tag="ACHJ1G_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4764266.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19803..20456)
                     /gene="fdnI"
                     /locus_tag="ACHJ1G_002421"
     CDS             complement(19803..20456)
                     /gene="fdnI"
                     /locus_tag="ACHJ1G_002421"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4764267.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20449..21333)
                     /gene="fdnH"
                     /locus_tag="ACHJ1G_002422"
     CDS             complement(20449..21333)
                     /gene="fdnH"
                     /locus_tag="ACHJ1G_002422"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4764268.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(21346..24393)
                     /gene="fdnG"
                     /locus_tag="ACHJ1G_002423"
     CDS             complement(21346..24393)
                     /gene="fdnG"
                     /locus_tag="ACHJ1G_002423"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23806..23808),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4764269.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24625..25506
                     /gene="yddG"
                     /locus_tag="ACHJ1G_002424"
     CDS             24625..25506
                     /gene="yddG"
                     /locus_tag="ACHJ1G_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4764270.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6260 a         6504 c         6481 g         6360 t
ORIGIN      
        1 gttatttaaa ttaagcctgt aatgccttgc ttccattgcg gataaatcct acttttttat
       61 tgccttcaaa taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct
      121 cagaactact cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac
      181 gatttccgct gcacgaaatg cgcgatgatg tcgcattcca gattatcaat gatgaattat
      241 atcttgatgg caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa
      301 acgtccataa attgatggat ttgtcgatca ataaaaactg gatcgacaaa gaagaatatc
      361 cgcaatccgc agccatcgac ctgcgttgcg taaatatggt tgccgatctg tggcatgcgc
      421 ctgcgccgaa aaatggtcag gccgttggca ccaacaccat tggttcttcc gaggcctgta
      481 tgctcggcgg gatggcgatg aaatggcgtt ggcgcaagcg tatggaagct gcaggcaaac
      541 caacggataa accaaacctg gtgtgcggtc cggtacaaat ctgctggcat aaattcgccc
      601 gctactggga tgtggagctg cgtgagatcc ctatgcgccc cggtcagttg tttatggacc
      661 cgaaacgcat gattgaagcc tgtgacgaaa acaccatcgg cgtggtgccg actttcggcg
      721 tgacctacac cggtaactat gagttcccac aaccgctgca cgatgcgctg gataaattcc
      781 aggccgacac cggtatcgac atcgacatgc acatcgacgc tgccagcggt ggcttcctgg
      841 caccgttcgt cgccccggat atcgtctggg acttccgcct gccgcgtgtg aaatcgatca
      901 gtgcttcagg ccataaattc ggtctggctc cgctgggctg cggctgggtt atctggcgtg
      961 acgaagaagc gctgccgcag gaactggtgt tcaacgttga ctacctgggt ggtcaaattg
     1021 gtactttcgc catcaacttc tcccgcccgg cgggtcaggt aattgcacag tactatgaat
     1081 tcctgcgcct cggtcgtgaa ggctacacca aagtacagaa cgcctcttac caggttgccg
     1141 cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt acgggtcgcc
     1201 cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat ccgggataca
     1261 ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg gccttcactc
     1321 tcggcggtga agccaccgac atcgtggtga tgcgcattat gtgtcgtcgc ggcttcgaaa
     1381 tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat ctcagcgatc
     1441 acccgaaact gcagggtatt gcccagcaga acagctttaa acatacctga taacgtttaa
     1501 cggtaacggt gtcccgaaac gaacccgttt cgggacaatt tccaaagtct gttcactggc
     1561 attagcaacg gaaaatattg ttctgaatac gcttcagaac aaaacaggtg cggttccgac
     1621 aggaataccg ttttaggggg ataatatggc tacatcagta cagacaggta aagctaagca
     1681 gctcacatta ctcggattct ttgccataac ggcatcgatg gtaatggctg tttatgaata
     1741 ccctaccttc gcaacatcgg gcttttcatt agtcttcttc ctgctattag gcgggatttt
     1801 atggtttatt cccgtgggac tttgtgctgc ggaaatggcc accgtcgacg gctgggaaga
     1861 aggtggtgtc ttcgcctggg tatcaaatac tctggggccg agatggggat ttgcagcgat
     1921 ctcatttggc tatctgcaaa tcgccattgg ttttattccg atgctctatt tcgtgttagg
     1981 ggcactctcc tacatcctga aatggccagc gctaaatgaa gaccccatta ccaaaactat
     2041 tgcagcactc atcattcttt gggcgctggc attaacgcag tttggtggca cgaaatacac
     2101 ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg ttacctgcat ttattttgat
     2161 cgcattagcg gctatttatc tgcactccgg tgcccccgtt gctatcgaaa tggattcgaa
     2221 gaccttcttc cctgacttct ctaaagtggg caccctggtt gtatttgttg ccttcatttt
     2281 gagttatatg ggcgtagaag cttccgcaac ccacgtcaat gaaatgagta acccagggcg
     2341 cgactatcca ctggctatgt tactgctgat ggtggcggca atctgcttaa gctctgttgg
     2401 tggtttgtct attgcgatgg tcattccggg taatgaaatc aacctctccg caggggtaat
     2461 gcaaaccttt accgttctga tgtcccatgt ggcaccggaa attgagtgga cggttcgcgt
     2521 gatctccgca ctgctgttgc tgggtgttct ggcggaaatc gcctcctgga ttgttggtcc
     2581 ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg ccagcggcat tcgctaaaat
     2641 gaacaaaaat ggcgtaccgg taacgctggt catttcgcag ctggtgatta cttctatcgc
     2701 gttgatcatc ctcaccaata ccggtggcgg taacaacatg tccttcctga tcgctctggc
     2761 gctgacggtg gtgatttatc tgtgtgctta tttcatgctg tttattggct acattgtgtt
     2821 ggttcttaaa catcctgact taaaacgcac atttaatatc cctggtggta aaggggtgaa
     2881 actggtcgtg gcaattgtcg gtctgctgac ttcaattatg gcgtttattg tttccttcct
     2941 gccgccggat aacatccagg gtgattctac cgatatgtat gttgaattac tggttgttag
     3001 tttcctggtg gtacttgccc tgccctttat tctctatgct gttcatgatc gtaaaggcaa
     3061 agcgaatacc ggcgtcactc tggagccaat caacagtcag aacgcaccaa aaggtcactt
     3121 cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg atgaatgaca agaaacacta
     3181 accatgtctg aatggttata agacaaggga gcgataagtc atcgctccct ttttcgtgct
     3241 tgctgtcgta ttgacttaac cgggataagt acgagaatga gcgcacatct gtttaccgga
     3301 aaccagcaca tatggatatc tgctcccgaa acaagaaatt aacgattaga agaccagcga
     3361 tactagttgc actggcactt ttactgtgta gttgtaaaag cacgcctcca gagtccatgg
     3421 tgacaccacc agcaggttca aagccaccag ccacgacgca acaatcgtca caaccgatgc
     3481 gtggcatctg gctggccacg gtttctcggc tcgactggcc accggtttcc tcggttaaca
     3541 ttagtaaccc caccagccgg gcccgtgtac aacaacaggc gatgatcgac aaactggatc
     3601 atctacaacg tctcggcata aacacggtct ttttccaggt caagccggac ggtaccgccc
     3661 tgtggccatc gaaaattttg ccgtggtccg atcttatgac cggtaagatt ggtgaaaatc
     3721 cgggttacga tccgctgcaa tttatgctcg acgaagccca caagcgtggg atgaaagtac
     3781 acgcctggtt taacccctat cgcgtatcgg ttaatacgaa gcccggtact atcagggaac
     3841 tgaatagcac tctgcctcaa caaccggcga gcgtctatgt gcaacaccgc gactggatca
     3901 gaacgtctgg cgatcgcttt gtcctcgacc cgggcatccc tgaggttcag gactggatca
     3961 catcaatagt cgcagaagtg gtttcccgct atcccgtaga tggcgtgcag tttgacgact
     4021 atttctatac ggagtcaccg ggttcacggc taaatgataa cgaaacgtac cgtaaatacg
     4081 gaggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa tactcagcag ttaattgcaa
     4141 aggtatcgca caccattaaa agcattaagc cgggagtcga atttggtgtt agcccggcag
     4201 gcgtgtggcg taaccgatca cacgatccgc tcggttccga tacccgaggc gcggcagcct
     4261 atgacgaatc ctacgctgac acccgtcgat gggtggaaca aggattgctg gattacattg
     4321 ctccccaaat ttactggccg ttctcacgga gtgccgcgcg ttatgacgtg ttggcaaaat
     4381 ggtgggcgga tgtcgttaaa ccgaccagga cccgcctgta tatcggtatc gccttctata
     4441 aagtgggtga accttcaaag atagagccag actggatgat taacggcggc gtacaggaac
     4501 tgaaaaagca gctcgatctt aacgatgcgg taccagaaat tagcggcacc atcttgttcc
     4561 gtgaggacta tctgaataaa ccacagactc aacaagcggt cagctatctg caaagtcgtt
     4621 ggggcagtta aactcccatt cttactttgt aacaagccgg tgctttaccc accggcttgt
     4681 taccctcttg aactatccca ctactaatca tgcttactaa agtcaaatta accacgcaat
     4741 taaccacact tagttaaaca gccataaatc ccctaataaa aatgtatgaa tatgaaaaaa
     4801 catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt ctgaagaaca
     4861 gtagcgtaga ttaaaactgt cctaagtcgc ttatttcttt tcagtatatc ttcatatttc
     4921 aggagaatat ggtgcagtta gtcgatctcg cacgttgcgt ttcgttttga ttccgtctga
     4981 tttcccagcc ttataagggt gtgaattatg gagatgtatt ttaaaagaat gaaagatgag
     5041 tggaccggac ttgtcgaaca ggcagatcct ctcattcgtg ctaaagccgc agaaattgcc
     5101 gttgcgcatg ctcattatct gagtatcgag ttttatcgaa ttgtccgcat cgacccgcat
     5161 gccgaagaat tcttgagtaa tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc
     5221 tggattatta acgtgctttc tgcccaggtt gacgatgtcg aaaggctaat acaaatccag
     5281 cataccgtcg cggaagtgca tgcccgcata ggaattccgg tagaaattgt cgagatgggg
     5341 tttcgggtgc tgaaaaaaat cctctatccg gtaatcttct cttcggatta ttctgccgca
     5401 gaaaaacttc aggtctacca tttctcgatt aacagtattg atatcgctat ggaagtgatg
     5461 acccgcgcgt ttacctttag tgacagtagt gcctcaaagg aagatgaaaa ctatcgtatc
     5521 ttctcgttac tagaaaacgc cgaagaagaa aaagaacggc aaatagcctc aatactttca
     5581 tgggaaatag atattatcta taaaatcctg ctggattctg atttaggcag tagtttgcct
     5641 ttaagccagg ctgattttgg cctgtggttt aaccataaag gtcgacatta ttttagtggc
     5701 attgccgaag taggccatat ctcccgtctg attcaggatt tcgacggtat attcaatcaa
     5761 accatgcgta acaccaggaa tttgaataac agaagtctgc gggtgaaatt tttattacag
     5821 ataagaaata ctgtatcgca aattattacc ttgctgcgtg aattgtttga agaagtatcg
     5881 cgccacgaag tcggtatgga tgtactgacg aaattactta accgccgttt cctgccgact
     5941 atcttcaaac gcgaaattgc ccatgccaac cggaccggta caccactgtc agtgctgatt
     6001 attgacgttg ataaattcaa agagatcaac gatacgtggg gccataacac tggcgatgaa
     6061 attctgcgta aagtctctca ggccttttat gacaacgtcc gcagtagtga ttatgttttt
     6121 cgctacgggg gcgatgaatt tatcattgtt ttgactgaag cttctgaaaa cgaaacgtta
     6181 cgtaccgcag aacgtattcg cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc
     6241 gaggatattg ccctctcact ttccatcggt gccgccatgt ttaatggtca tcctgactat
     6301 gagcgcctca ttcaaatagc cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac
     6361 cgtgttgaac tctggaaagc cagtctttag atgcgccagg atgcagaggt aatcatgaag
     6421 ctaaccgatg cggataatgc cgccgatggc atttttttcc ccgcccttga gcaaaatatg
     6481 atgggtgcgg tgttaattaa cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag
     6541 aagctctggg ggtacaaacg tgaagaagtc attggcaata acattgatat gctgattccg
     6601 cgggatttgc gtcctgcgca tcctgaatac attcgtcata accgtgaagg cggtaaagcg
     6661 cgtgtcgagg ggatgagtcg ggagctgcag ctggagaaaa aagacggcag taaaatctgg
     6721 acccgttttg cgctatcgaa agtgagcgcc gaggggaaag tttattacct ggcgctggta
     6781 cgggatgcca gcgtagaaat ggcgcaaaaa gaacagactc gacaattgat tattgccgtt
     6841 gaccatctcg accgaccggt gattgtcctc gatccggaac gccatattgt gcagtgcaat
     6901 cgcgcattta ccgaaatgtt tggttactgc attagcgaag ccagcggtat gcagcccgat
     6961 acactcctga acattcctga attccctgcc gataaccgca ttcgtttaca acagttgcta
     7021 tggaaaaccg cccgcgatca ggacgaattt ctgctgttga cgcgcaccgg tgaaaaaatc
     7081 tggattaaag cctctatcag cccggtttat gacgtgctcg cgcatctgca gaacctggta
     7141 atgactttct cggatatcac cgaagaacgg caaatccgcc agcttgaagg caatattctc
     7201 gccgccatgt gcagcagccc gccatttcat gaaatggggg aaatcatttg tcgtaacatc
     7261 gaatctgttc tcaacgaatc gcatgtttcg ctgttcgcac tgcgcaacgg gatgccgata
     7321 cactgggcgt catcttcgca cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc
     7381 attcgtcagc gtgatggcgc gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca
     7441 gaaaccagcg cctttatcga acgcgtggca gatatcagtc agcatatggc cgcgctggcg
     7501 ctggaacagg aaaaaagccg ccagcatatt gaacaactca tccaatttga tccgatgacc
     7561 ggtctgccaa atcgcaataa cctgcacaat tacctcgatg acctggtcga caaagccgtc
     7621 tctccggtgg tgtatctcat cggtgttgac catattcagg atgtgattga tagccttggc
     7681 tatgcgtggg ccgatcaggc attgctggaa gtggtcaatc gctttcgtga aaaactcaaa
     7741 ccggatcagt atctctgtcg tatcgaaggt acgcagtttg tcctcgtgag cctggaaaac
     7801 gacgtcagta acattaccca gatcgccgat gagctacgga atgtggtcag caagccgata
     7861 atgattgacg ataaaccatt cccgcttacc ttgagtattg gcatcagcta cgacgtgggt
     7921 aaaaaccgcg attacctact ctccactgct cacaatgcaa tggattatat tcgcaagaat
     7981 ggcggtaacg gctggcagtt cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg
     8041 gttttaggcg cagcgctgaa agaagcgatt agcaataacc aactgaaact ggtttaccag
     8101 ccgcaaatct tcgcagaaac gggtgaactg tacggcatcg aagcccttgc tcgctggcac
     8161 gatccccagc atggtcatgt acccccttca cggtttattc ctctcgcaga agagattggt
     8221 gaaatcgaaa atattgggcg ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg
     8281 cgtagccaga atattcatat cccggcgtta tccgtgaact tgtcggcgct gcactttcgc
     8341 agtaatcagc tgcctaatca ggtgtctgat gcaatgcaag cctggggtat tgacggccac
     8401 cagctgacgg tagaaatcac ggaaagcatg atgatggaac acgataccga aatctttaag
     8461 cgcattcaga tcctgcgtga tatgggcgtg ggcttatcgg tagatgattt tggtacgggc
     8521 ttttccggat tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagt
     8581 tttgtcgatc gttgtctgac cgaaaaacgc atccttgcct tacttgaagc cattaccagc
     8641 attgggcaaa gcctcaattt aaccgtcgtg gcggaaggcg tcgaaaccaa agagcaattt
     8701 gagatgctac gcaagatcca ctgtcgcgtt attcagggat atttcttttc ccgcccccta
     8761 cccgccgaag aaattccagg ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt
     8821 cctctcgccc gcactcgcgg gttttcattt aacgtgacac tgtcacttaa atactgtgat
     8881 ttcagctacg attccgggag attccttcct taacttcccc tcttgcacca aaactgcact
     8941 acgctgcaca aatgttgatc aaaagttaaa aaatataact ttaagccagc attctggcgc
     9001 ttatcccggc atggcatgag atctgcataa gcggaaagcg cagcaatttt tgtcttatac
     9061 aggaaccgtt tatgtcggat accaccgaac tggttgattt agccgtaatc ttccctgatc
     9121 tggagatcga attgaaatac gcctgcgctg ataacatcac aggtaaagct atttatcagc
     9181 aagcgcgttg tctgctacac aaggatgcga ttaccgcgct ggcgaaaagt atcagtatcg
     9241 cccagctgtc aggcttacaa ctggtgattt acgatgcgta tcgcccacaa caagcacagg
     9301 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta
     9361 atcacagccg tggcacggcg atcgacctga cgcttcgtga tgagcacggg aacatcctcg
     9421 atatgggcgc agggttcgat gaaatgcacg agcgctccca tgcctatcac ccttccgtcc
     9481 cgcccgccgc tcagcgcaat cggctgttgc tgaatgcgat aatgactggt ggcggttttg
     9541 tcggtatctc cagcgaatgg tggcacttcg aattacccca ggcagcgagt taccctctgc
     9601 tcgctgatca attctcttgt tttatatccc ctggcacaca gcacgtcagt taatcaggaa
     9661 actgtcatga agagatcgat atcgtttcgt cccacattgc tcgcgctcgt ccttgccaca
     9721 aatttcccgg tttcgcacgc cgccgtaccg aaagatatgc tggtgattgg taaggccgcc
     9781 gatccacaaa ccctcgaccc ggcggtaaca atagataata acgactggac ggtgacctac
     9841 ccgtcttatc agcggctggt tcagtacaaa acggacggtg ataaaggctc aaccgacgtt
     9901 gaaggcgatc tggcaagtag ctggaaagcg tctgacgatc aaaaagagtg gacgttcacc
     9961 ctgaaagata atgctaaatt tgccgatggc acacctgtca ctgccgaagc agtaaaactt
    10021 tcttttgagc ggctactaaa aatcggccag gggccagcag aagcatttcc caaagattta
    10081 aagattgatg ctcccgacga acatacggtg aagtttaccc ttagccaacc attcgcaccg
    10141 ttcctctaca cgctggcgaa tgacggtgca tccattatca atccggcggt cttaaaggaa
    10201 catgcagcgg atgatgctcg cggcttcctc gcgcaaaata ccgccggttc cggaccattt
    10261 atgctgaaaa gctggcaaaa aggtcagcaa ttagttctgg tgccaaatcc gcattacccc
    10321 ggcaataaac cgaactttaa gcgagtatcg gtaaaaatta tcggtgaaag tgcctcccgt
    10381 cgcctgcagc tctcccgtgg cgacattgac attgccgatg cgctgccggt ggatcaactc
    10441 aacgccctga agcaggaaaa taaagtcaat gtggcagagt atccgtcact gcgcgttacc
    10501 tatctgtatc tgaataacag caaagcgcct cttaatcagg cggatctacg tcgggccatt
    10561 tcctggtcta ccgattatca gggcatggtt aacggcattc tgagtggtaa cggaaaacag
    10621 atgcgcggcc cgattccgga aggcatgtgg ggctacgatg cgacggcaat gcaatacaac
    10681 catgacgaaa cgaaagccaa agccgaatgg gataaagtga cgagcaaacc caccagcctg
    10741 acgtttctct actccgataa cgatccgaac tgggagccta ttgctttggc gacacaatcc
    10801 agtctcaaca agctgggtat caatgtgaag ctggaaaagc tggcgaacgc caccatgcgc
    10861 gacagagtgg gtaaaggtga ttacgacatt gcgattggca actggagtcc ggattttgcc
    10921 gacccgtata tgtttatgaa ttactggttt gagtcagaca aaaaaggtct gccgggtaac
    10981 cgctcgttct atgaaaacag tgaggtcgat aagttactgc gcaatgcgct tgcgaccacc
    11041 gaccagacgc agcgtacccg ggactaccag caggcacaga aaatcgtcat tgatgacgct
    11101 gcttatgtgt acctgttcca gaaaaactac caactggcga tgaacaaaga ggtgaaaggc
    11161 tttgtgttca atcccatgct ggaacaggtc ttcaatatca ataccatgag taaataacgt
    11221 gaccttctgg agtattttac gccaacgctg ctgggggctg gtgctcgtgg tggcgggcgt
    11281 ctgcgtgatt acgtttatta tctcgcatct gatccctggc gatccggcgc ggttactggc
    11341 gggtgaccgc gccagcgatg ctatcgtgga aaatattcgc cagcaactgg gactggacca
    11401 gccactgtat gtacagtttt accgctacgt cagcgacctg tttcatggtg acctgggaac
    11461 atccattcgt accgggcgtc cggtgctgga agagttgcgt atatttttcc cggcgacgct
    11521 ggagctggct tttggtgccc tgctgctggc actcctgatt ggcatcccgc tgggcatact
    11581 ctctgcagtc tggcgaaatc gctggctgga tcatctggtg cgaataatgg ccattaccgg
    11641 aatctccaca cctgcgttct ggcttgggct gggcgtcatt gtgctgtttt atggtcatct
    11701 gcaaattctt cccggcggcg gaaggcttga tgactggctg gatccaccaa cgcacgttac
    11761 cggcttttat ctgctcgatg cgctgcttga aggcaacggt gaagtcttct tcaatgcgtt
    11821 gcaacatctc atcttaccgg cattaacgct ggcgttcgtt cacctgggaa ttgtcgctcg
    11881 ccagatccgc tcagcgatgc tggaacaatt gagtgaagac tacattcgta ccgcccgggc
    11941 cagcggcttg cccggctggt atatcgtttt atgttatgcg ctacccaatg cgttgatccc
    12001 atcgattacc gtattgggtt tggcgctggg cgatttgttg tatggcgcag tgctcaccga
    12061 aaccgttttt gcctggcccg gaatgggtgc atgggtagta acatcaatac aggcgctcga
    12121 cttcccggca gtgatgggct ttgccgtcgt ggtttcattt gcttatgtgc tggtcaacct
    12181 ggtggtggat ttgctctatt tgtggattga tccgcgtatc ggacgtggag gtggtgaatg
    12241 atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca
    12301 aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta
    12361 ttaatgctat tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt
    12421 gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg
    12481 ggacgcgatc tgttcagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta
    12541 gtcgtggttg ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt
    12601 ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg
    12661 ttggtactga caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg
    12721 gcgcttgcta ttgtgcgaat tcccttttat gtgcgcctgg cgcgggggca agcattagtt
    12781 gtacgccagt atacctatgt tcaggcggcg aaaacctttg gtgcgtcgcg ttggcatctg
    12841 atcagctggc atattttacg taactccctg ccgccgctga tcgtgcaggc atcgctggat
    12901 atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa
    12961 ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg
    13021 tggtattgcg catttccggg ggcagcgatt ttgcttaccg ccgttgggtt taatctcttt
    13081 ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac
    13141 ccgttctgga cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg
    13201 cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat
    13261 ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctacag acgggcagtt
    13321 attgcgtaca tcgggggcag atttcactgc tgggagacga tgttcttaac gctcgggaaa
    13381 aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg
    13441 ccctcaatcc gacacttcga ataggtcttc agatgatgga cgtgatccgc catcatcaac
    13501 caataagtcg tcgggaagcc agagctaaag cgattgccct gctggaagag atgcaaatcc
    13561 cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc
    13621 gggtaatgat tgcgctggca ttctcctgcg agccgcaatt gattattgcc gacgaaccga
    13681 ctacggcgct ggacgtcacg gtgcagttgc aggtactgcg tttgcttaaa cataaagccc
    13741 gcgccagtgg aactgcggtt ctgttcatca gccatgatat ggccgtggtg tcgcaactgt
    13801 gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga aagcggcgtg acggcagatg
    13861 ttatccatca tccccggcat ccgtatacca ttggtttgct ccaatgcgca ccagaacatg
    13921 gagtaccacg ccagctatta cccgccattc cagggacggt accaaacctc acccatttgc
    13981 ctgaaggctg cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc
    14041 cggcgctgac agcgtgtggt gacaacaacc agcgctgcgc ctgttggtat ccgcagcagg
    14101 aggtcataag tgtctgacac gttattaacg ttacgcgacg tccatatcaa tttcccggcc
    14161 cgtaaaaact ggcttggtaa aactacggaa catgttcatg ccattaatgg tattgattta
    14221 cagatccgcc gtggtgaaac cttagggatc gtcggcgagt caggctgcgg caaaagcacc
    14281 ctcgcacagc ttttaatggg catgctgcaa ccgagccacg ggcagtacat ccgttctggc
    14341 tcacaacgca ttatgcagat ggtgtttcag gacccgctct cttcgctcaa tccgcgctta
    14401 ccggtgtggc gcatcatcac agaaccgctc tggatagcta agcgtagtag tgaacaacag
    14461 cggcgagcgt tggcagagga gctggctgtg caggtgggta ttcgtccgga gtatctcgac
    14521 cgcctgcctc atgcgttctc cggcgggcag cggcaacgca tcgccattgc cagagcactc
    14581 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
    14641 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
    14701 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
    14761 ctcgggcaga ttgtggaact aggggaagcg caacaggtgt tgactgttcc ggcacatcca
    14821 tatacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
    14881 gcattacgta aaacggatct gccgggaaac cgcacgttgc cgcaaggctg ttttttctac
    14941 gaacgttgcc ctctggcaac ccacggatgt gaagtccggc aatcattaac aataagggag
    15001 gacggacgtg agatccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
    15061 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tcaagcgtaa
    15121 tttccgcttt cagtacctga gaaaccgggc atcctgcttt tgctttctgg atgatgccgt
    15181 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
    15241 tcgcaaaacc ggcatccact ttatccagcg acacatcggc ggtggtatca atcgatgttg
    15301 gcgtaaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
    15361 ctgcgccaat cagttcttca gggttagttc ctttttcgcc ttcaaaacgc gtgttaaatc
    15421 catacggctg ttggttcagc acaccactct cggtggatac tgttcccttc ccgcgtttga
    15481 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
    15541 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
    15601 ataaaaatcc ggcagaaata ttacttctgc acttaaatta aatttccaca atagataacc
    15661 gggaatatat aacccaagag atattgcatt aatattaatc aactcaccac aataataata
    15721 cctatcaata tcatgaggtt aaaatataaa aatcatcaac caggactaat cttaacaacg
    15781 aagcggcaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
    15841 cataaataca cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac
    15901 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
    15961 cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat
    16021 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacgct
    16081 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
    16141 cggcgatgtt gtccttaaaa ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt
    16201 caggcacgtc atattcttgg actggaccat aaaatttcta accagcgcaa aatagttacc
    16261 gaaggtgaca aatccagcgt ggtaaataat ccaaccggca gaaaacgccc cgctgaaaag
    16321 taattcataa ccatcagtcc tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt
    16381 ttgtttttat ctgctctata cttggggccg acgccctggc ggtaaagcaa agacgataaa
    16441 agcgtgccag ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa
    16501 cagcgttcgc tttatatccc ttacgctggc cctgtactgc tggaatttcc gttgttgaat
    16561 aaaggcagtg ccttcagcat ggaagaacgc cgtaacttca acctgctggg gttactgccg
    16621 gaagtggtcg aaaccatcga agaacaagcg gaacgagcat ggatccagta tcagggattc
    16681 aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc aggacaccaa cgaaaccctc
    16741 ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc ctgttattta taccccaacc
    16801 gtcggcgcag cctgtgagcg tttttctgag atctaccgcc gttcacgcgg cgtgtttatc
    16861 tcttaccaga accggcacaa tatggacgat attctgcaaa acgtgccgaa ccataatatt
    16921 aaagtgattg tggtgactga cggtgaacgt attctggggc ttggtgacca gggcatcggc
    16981 gggatgggca ttccgatcgg taaactgtcg ctctataccg cctgtggcgg catcagcccg
    17041 gcgtataccc ttccggtggt gctggatgtc ggaacgaaca accaacagct gcttaacgat
    17101 ccgctgtata tgggctggcg taatccacgt atcactgacg atgaatacta tgaattcgtt
    17161 gatgaattta tccaggctgt gaaacaacgc tggccggacg tgctgttgca gtttgaagac
    17221 tttgctcaaa aaaatgcgat gccgttactt aaccgctatc gcaatgaaat ttgttctttt
    17281 aacgatgaca ttcagggcac tgcggcagta acagtcggca cactgatcgc agccagccgc
    17341 gcggcaggtg gtcagttaag cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga
    17401 tgcggcattg ccgaaatgat catcgcccag acccagcgcg aaggattaag cgaggaagca
    17461 gcgcggcaga aagtctttat ggtcgatcgc tttggcctgc tgacggacaa gatgccgaac
    17521 ctgctacctt tccagaccaa actggtgcag aagcgcgaaa acctcagtga ctgggatacc
    17581 gacagcgatg tgttgtcact gctggatgtg gtgcgcaatg taaaaccaga tattctgatt
    17641 ggcgtctcag gacagaccgg gctgtttacg gaagagatca ttcgtgagat gcataaacac
    17701 tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt cacgcgtgga agccacaccg
    17761 caggacatta tcgcctggac cgaaggtaac gcgctggtcg ccactggcag cccgtttaat
    17821 ccagtggtat ggaaagataa aatctaccct atcgcccagt gtaacaacgc ctttattttc
    17881 ccaggcatcg ggctgggtgt tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg
    17941 atgtcagcaa gtgaaacgct tgctcagtat tcgccgctgg tgctgaacgg cgaaggtctg
    18001 gtactaccgg aactgaaaga tattcagaaa gtctcccgcg caattgcgtt tgcggttggc
    18061 aaaatggcgc agcagcaagg cgtggcggtg aaaacgtctg ccgaagctct gcaacaagcc
    18121 attgacgata atttctggca agccgaatac cgcgactacc gccgtacctc catctaagcc
    18181 tgcgcccggt agtgaaggct accgggctat ttccctctcc ctttttcaga tctcatccat
    18241 actgggtagt ggcgaataaa tctcatttgc ctcacctgct atgcagaaca tcatccgaaa
    18301 aggaggaact atgaaggctg cagttgttac gaaggatcat catgttgacg ttacggataa
    18361 aacactgcgc tcactgaaac atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg
    18421 tcataccgat cttcatgtta agaatggcga ttttggtgac aaaactgggg taattctggg
    18481 ccatgaaggc atcggtgtgg tggcagaagt gggtccaggt gtcacctcat taaaaccagg
    18541 cgatcgtgcc agcgtggcgt ggttctacga aggatgcggt cattgcgaat actgtaacag
    18601 tggtaacgaa acgctctgcc gttcagttaa aaatgccgga tacagcgttg atggcgggat
    18661 ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa gtgccagatg gtctggactc
    18721 ggcggcggcc agcagcatta cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc
    18781 aaaaattcgt cccggtcagt ggattgctat ctacggtctt ggcggtctgg gtaacctcgc
    18841 cctgcaatac gcgaagaatg tctttaacgc caaagtgatc gccattgatg tcaatgatga
    18901 gcagttaaaa ctggcaaccg aaatgggtgc agatttagcg attaactcac gcaccgaaga
    18961 cgccgccaaa attgtgcagg agaaaaccgg tggcgctcac gctgcggtgg taacagcagt
    19021 tgctaaagct gcgtttaact cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc
    19081 tgtcggtctg ccgccggagt ctatgagcct ggatatccca cgccttgtgc tggatggcat
    19141 tgaagtggtc ggttcgctgg tcggcacgcg ccaggatcta accgaagcct tccagtttgc
    19201 cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat
    19261 ctttaccgag atggaagaag gcaaaatccg tggccgtatg gtgattgatt tccgccgtta
    19321 agaggccttt gctgcgagtg ccagtcctgg tgtcgcagca tcgcactctc tccgacatta
    19381 aatggctaaa tggattattg ttccttccta cgcaacaata atgatagtgt tcctgatgaa
    19441 ggaacactac tgagagtaaa gacactgatg aaaatggcca atcatccccg cccgggggac
    19501 attattcagg aatcactgga cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg
    19561 gaaattgctc cctcaacggc aagccgattg ctgaccggaa aagcagcttt gacaccagaa
    19621 atggcgatta aactttccgt ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa
    19681 aacgcctgga gtctggcaga ggcagaaaaa acggtggatg tgtcgcgact acgccgtctg
    19741 gttacgcaat aaaaaaatgg cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga
    19801 gattatatcc cttcttcact ctcttttttc gcttctgcct tctcgatttc acgataccag
    19861 cgcggatggt gtttcttcgc ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc
    19921 gaccctttca cccagaatgc catatacata tggatcagga tggcgtggat caggatgata
    19981 cccgcagccg cgtggatcag caggctgtag cgaacaacct gcatcgggaa gtactgcgca
    20041 aagtacggac gccagataat caccccggtc accagcagca cgaaaatcat gctcatgatc
    20101 gaccagaaca tcatcttttg cccggcgttg tacttaccga catccgccac tttatgctca
    20161 ttgcctttca atacttcgac aatgttcaac agccacggaa tatctttctt atccgggatg
    20221 ttgtgatgca caaaacgcac aaacataaac atcagtgcga cgaaaatcgc aatgccgaaa
    20281 aacgggtgca aaatgcgtcc catctgcggc gtaccgaagg tttgcgtcag ccattgcagc
    20341 gtcgggaaga agaacgaaat cccggacagc gccaccagga agaagcaaat caccacggtc
    20401 cagtgacagg cgcgatcaat aaatttggtg cgcacaatca ttttcgactt actcatgatg
    20461 atcctcctcg tcatcgtcca cttccttatt cgggccaata ccgatgtagt ggaaaatcaa
    20521 cccggcaaaa gtggcaataa agccagccgc tgccagcggt ttcaacgcgc ctttccacag
    20581 gcttaccgag gtgtcgatct tcggatcttt cggcagaccg tgatacagct ccggctgatc
    20641 ggcgtgatgc agcacgtaca taacgtgcgt accaccgacc ccttccgggt tgtagacgcc
    20701 agcatgttcg taaccacgcg ctttcagttt cgccacgcgc tgttccgcca gctccagcat
    20761 ctccttcttg gtgccgaagt ggatagcccc ggtcggacag gttttcacac aagccggttc
    20821 ctggccgacg ctgacgcgat cgacgcagag cgtgcattta tatacccggt tatcctcttt
    20881 gttgaggcgc ggaatattaa acggacaccc ggcaatgcag taaccacagc cgatgcagtt
    20941 ttccgactgg aaatcgacaa tcccgttagc gtactgaatg attgcaccag cagacgggca
    21001 cgccttcagg cagccgggat cttcacagtg catacagccg tctttacgga tcagccactc
    21061 cagcttgccg ttctgttcgg tttcgctaaa gcgcatcacc gtccaggact tggcgctcag
    21121 atcggcgggg ttatcgtaaa ccccgacgca gtgccccact tcatcacgga tgtcgttcca
    21181 ctccgaacac gccacctgac aggctttaca gccgatacag gtggaaacgt cgataagttt
    21241 tgcgacttct gctttgtaat cacgcacctg agaaggcggc gtgatggagt tagttgcgga
    21301 ccttttgata atgtcctgcg tttccatagc catctgttcg cccccttacg ccttctcgat
    21361 gttgactaag aacgctttat attccggcgt ttgcgagttt gcatcaccga cattcggcgt
    21421 cagagtgtta gcgatataac ctttacgcgc gacaccctca aagccccagt ggattggaat
    21481 acccaccgtt tcaacctgct gaccatttac attcagtggt ttcagacgac gcgttaccac
    21541 agccaccgcg cggataaagc cacgcttgct tgagacagtg acacgatcgc cattattaat
    21601 gcctttcgcc gccgccagcg tttcgctgat ttccacaaac tgttccggct gagcaattgc
    21661 gttgagcaat gcgtgcttgg tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc
    21721 cacatacggg aactgctctt ttttacccat ccgcagtgcg tcttgttcat acagacgaac
    21781 aaccgggtta gacaccacgt tcggatgcag cgggttagtg cccagcggcg tttcaatcgg
    21841 ctcgtagtgt tccgggaacg gaccttccgc cattttgttg atggcaaaca ggcgtcccat
    21901 cccttccggc tgcatgataa acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc
    21961 aggaatatcg ttacccgtcc acttgctgcc gttccactgg atcagcatcc gtttcggatc
    22021 ccacggttta ccgttgatat ccgccgaagc acggttgtac agcacgcgac ggttgagcgg
    22081 ccacgcccag gcccatccca gcgtattccc cagaccggac gggtcggagt tatcgcggtt
    22141 agccatctgg ttgccctgct ctgtccagct accggtgtag atccagcaag aagatgcggt
    22201 tgtaccgtca tcacgcagat gcgcaaagct actcagcaac tgacctttct tcgcaatcag
    22261 cacgccatta gcatcataga gatcttccag cgcatagccg ttgttctctt tagctacttc
    22321 gtcagattgc ggttcgtgcg gctgcttgta gttccagctc atcttcatca gcggttctac
    22381 gcctttacca ccttcggcct ggtacagctc gcgcaggtga tggtagatac ccgccagaat
    22441 ttcaccgtcg ttacgcgctt cgcccggcgc atcctgacct ttccagtgcc actgcagcca
    22501 gcgaccggag ttagcaatag aaccatcttc ttcagcaaag caggtcgaag gcagacggaa
    22561 tacttcagtc tgaatagacg ccggatcgac atcgttcgac tcgccgtggt tctgccagaa
    22621 ggttgaggtt tcagtcacca gcggatcgat aaccaccatg tacttcagct tgcttaggca
    22681 gctcaccact ttgtttttgt ccgggaagga cgcaaccggg ttaaagccct ggcagaaata
    22741 accggtgact ttgccttcat ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc
    22801 ccacttcggc agccagtcat agccccagtt gttctctttc tgcgcggcat cgccatagaa
    22861 agatttcatc aggctaacga agaacttcgg atagttgctc cagtagttca cctgaccagc
    22921 cagcgtcgct ttcggcgtgt tcgcttccag atacgactgc aaatcaacct gtttttctga
    22981 cggcagcgtc agataacctg gcaggctggt agagagcaga cctaagtcag tcagaccctg
    23041 aatgttggag tgaccacgca atgcattcac gccgccaccg gccataccca tgttaccgag
    23101 cagcaactgg atcatcgcca tagtacggat gttctgcgca cccacggtgt gctgcgtcca
    23161 gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg ctggtggagg ccagcacttc
    23221 acacactttc aggaagtcgg cttttggcgt accacagatg ttttcaacaa cgtccggcgt
    23281 gtagcgggaa acgtgcgctt tcagcaggtt ccacacacag cgcggatgag tcagtgtttc
    23341 atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc caggacgatt tatcgtattg
    23401 ccgtttttca gcgtcgtagc cgctgaacag accgtcttcg aaagcaaaat catcacgcac
    23461 cagcaggctg gcgttggtgt aatgcttaac gtattcggcg ttgattttgt cgttttcgat
    23521 caggtagcgc aaaacgccag acaggaacgt aatgtccgta ccggaacgaa taggtgcgta
    23581 aatatccgcc acagaagcgg tacgcgtaaa acgaggatcg acaacgatca aggttgcatc
    23641 gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt
    23701 accgcccatt accattacga cgttagcgtt tttgatatcc acccagtggt tggtcatcgc
    23761 accgcgacca aatgttggag caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg
    23821 gttgtctacc gccagcatcc cgagggagcg ggcaaatttc tgtgtcagca tcccggtttc
    23881 gttgctggca ccggaggcac acagcatacc ggtagaaagc caacggttta ccgttacgcc
    23941 ctgctcgttc ttttcaataa agttagcgtc acggtcagct ttcatcagct ttgcaatacg
    24001 ggagaatgct tcttcccagc taatgcgctg ccatttgtca gaacctggcg cacgatattc
    24061 cgggtagcgc agacggtttt cgctgttgac gtaatccagc aaaccggccc cttttgggca
    24121 cagcgcacca cggcttaccg gatgatccgg gtcaccttca atgtgataaa tcgcttctct
    24181 ggcgtttttc gcgccatcac ccaggctata catcaatagc ccgcaaccta cggaacagta
    24241 tgtgcaggtg ttacggatct ctttagcgcg taataatttg tagtttcgcg cctgagccag
    24301 tgcttgcttc ggggcaaagc ccaatgccgc taccgttgtt ccagccatac cgcccgcgca
    24361 gattttaaaa aattgtctgc gactgacgtc cattgctttc ctcttttttc aggggtatta
    24421 ctgcgcgagg aaactaacag taaaattctg aatctttttg cgcgaaatca aaaagaacac
    24481 gtgtttgcca caaaataatc cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa
    24541 cgcaccactg cctgacaggc cagttaaaaa aatgctataa aattcagctt aatttttaac
    24601 ggcaagagag acaaaacagc gagcatgaca cgacaaaaag caacgctcat agggctaata
    24661 gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagcga ggggctcggc
    24721 ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat cttcacggtt
    24781 ggatttccgc gtattcggca gattccgaaa ggctatttac tcgccgggag tctgttattc
    24841 gtcagctatg aaatctgtct ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg
    24901 attgaagtgg gtatggtgaa ctatctgtgg cccagcctga caattctctt tgccattctg
    24961 tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc cctggtcggc
    25021 gtctgctggg tgttaggcgg tgagaatggg ttacattatg atgaaatcat caataatatc
    25081 accaccagcc cattgagtta tttcctggcg tttattggtg cgtttatctg ggcagcctat
    25141 tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta
    25201 acgggagcaa gtctgtgggt ttactatttt ctcacgccac aaccagaaat ggtatttagc
    25261 acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc ttatgctgca
    25321 tggaatgtcg gtatactgca tggcaatgtc accataatgg cggtaggctc gtattttacg
    25381 cccgtacttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc gttctcattc
    25441 tggcaaggcg cgctaatggt ctgcggcggt tccctgctct gctggctggc gacacgtcgt
    25501 ggttaaaata attgccgggt ctacggcccg gcagttaatc agataaattc cgacaatatt
    25561 aaaaaataaa tttcaaatac cagtaattca ccttatatct cattt
//
LOCUS       DAZFDP010000057        25341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFDP010000057 DAZFDP010000000
VERSION     DAZFDP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1378
                     /gene="cdgI"
                     /locus_tag="ACHJ1G_002425"
     CDS             <1..1378
                     /gene="cdgI"
                     /locus_tag="ACHJ1G_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4764271.1"
                     /translation="IKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTLYN
                     ENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYL
                     VETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKK
                     TGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAF
                     LLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFV
                     VSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
                     FKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIA
                     ERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGG
                     NKVIIHHI"
     gene            1559..3049
                     /gene="dgcJ"
                     /locus_tag="ACHJ1G_002426"
     CDS             1559..3049
                     /gene="dgcJ"
                     /locus_tag="ACHJ1G_002426"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO4764272.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3092..3595
                     /gene="yeaK"
                     /locus_tag="ACHJ1G_002427"
     CDS             3092..3595
                     /gene="yeaK"
                     /locus_tag="ACHJ1G_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4764273.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3596..3700)
                     /gene="yoaI"
                     /locus_tag="ACHJ1G_002428"
     CDS             complement(3596..3700)
                     /gene="yoaI"
                     /locus_tag="ACHJ1G_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4764274.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3870..4316
                     /gene="yeaL"
                     /locus_tag="ACHJ1G_002429"
     CDS             3870..4316
                     /gene="yeaL"
                     /locus_tag="ACHJ1G_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4764275.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4273..5094)
                     /gene="nimR"
                     /locus_tag="ACHJ1G_002430"
     CDS             complement(4273..5094)
                     /gene="nimR"
                     /locus_tag="ACHJ1G_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4764276.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5191..6372
                     /gene="nimT"
                     /locus_tag="ACHJ1G_002431"
     CDS             5191..6372
                     /gene="nimT"
                     /locus_tag="ACHJ1G_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4764277.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6427..6774
                     /gene="yeaO"
                     /locus_tag="ACHJ1G_002432"
     CDS             6427..6774
                     /gene="yeaO"
                     /locus_tag="ACHJ1G_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4764278.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6796..7050)
                     /gene="yoaF"
                     /locus_tag="ACHJ1G_002433"
     CDS             complement(6796..7050)
                     /gene="yoaF"
                     /locus_tag="ACHJ1G_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4764279.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7233..8258
                     /gene="dgcP"
                     /locus_tag="ACHJ1G_002434"
     CDS             7233..8258
                     /gene="dgcP"
                     /locus_tag="ACHJ1G_002434"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO4764280.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8291..8389
                     /gene="yoaK"
                     /locus_tag="ACHJ1G_002435"
     CDS             8291..8389
                     /gene="yoaK"
                     /locus_tag="ACHJ1G_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4764281.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8392..8466
                     /gene="yoaJ"
                     /locus_tag="ACHJ1G_002436"
     CDS             8392..8466
                     /gene="yoaJ"
                     /locus_tag="ACHJ1G_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4764282.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8525..8773)
                     /gene="yeaQ"
                     /locus_tag="ACHJ1G_002437"
     CDS             complement(8525..8773)
                     /gene="yeaQ"
                     /locus_tag="ACHJ1G_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4764283.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9001..10209)
                     /locus_tag="ACHJ1G_002438"
     CDS             complement(9001..10209)
                     /locus_tag="ACHJ1G_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4764284.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            10217..10648
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_002439"
     CDS             10217..10648
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO4764285.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(10664..10852)
                     /locus_tag="ACHJ1G_002440"
     CDS             complement(10664..10852)
                     /locus_tag="ACHJ1G_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4764286.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10856..11215)
                     /gene="yeaR"
                     /locus_tag="ACHJ1G_002441"
     CDS             complement(10856..11215)
                     /gene="yeaR"
                     /locus_tag="ACHJ1G_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4764287.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11388..12026)
                     /gene="leuE"
                     /locus_tag="ACHJ1G_002442"
     CDS             complement(11388..12026)
                     /gene="leuE"
                     /locus_tag="ACHJ1G_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4764288.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(12153..13076)
                     /gene="dmlR"
                     /locus_tag="ACHJ1G_002443"
     CDS             complement(12153..13076)
                     /gene="dmlR"
                     /locus_tag="ACHJ1G_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4764289.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13179..14264
                     /gene="dmlA"
                     /locus_tag="ACHJ1G_002444"
     CDS             13179..14264
                     /gene="dmlA"
                     /locus_tag="ACHJ1G_002444"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4764290.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14515..16125
                     /gene="yeaV"
                     /locus_tag="ACHJ1G_002445"
     CDS             14515..16125
                     /gene="yeaV"
                     /locus_tag="ACHJ1G_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4764291.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            16157..17281
                     /gene="yeaW"
                     /locus_tag="ACHJ1G_002446"
     CDS             16157..17281
                     /gene="yeaW"
                     /locus_tag="ACHJ1G_002446"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4764292.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17337..18302
                     /gene="yeaX"
                     /locus_tag="ACHJ1G_002447"
     CDS             17337..18302
                     /gene="yeaX"
                     /locus_tag="ACHJ1G_002447"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4764293.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18356..19471)
                     /gene="rnd"
                     /locus_tag="ACHJ1G_002448"
     CDS             complement(18356..19471)
                     /gene="rnd"
                     /locus_tag="ACHJ1G_002448"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4764294.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19553..21238)
                     /gene="fadD"
                     /locus_tag="ACHJ1G_002449"
     CDS             complement(19553..21238)
                     /gene="fadD"
                     /locus_tag="ACHJ1G_002449"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4764295.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21443..22024)
                     /gene="yeaY"
                     /locus_tag="ACHJ1G_002450"
     CDS             complement(21443..22024)
                     /gene="yeaY"
                     /locus_tag="ACHJ1G_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4764296.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(22064..22759)
                     /gene="tsaB"
                     /locus_tag="ACHJ1G_002451"
     CDS             complement(22064..22759)
                     /gene="tsaB"
                     /locus_tag="ACHJ1G_002451"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4764297.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22817..24727)
                     /gene="yoaA"
                     /locus_tag="ACHJ1G_002452"
     CDS             complement(22817..24727)
                     /gene="yoaA"
                     /locus_tag="ACHJ1G_002452"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4764298.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24859..25203
                     /gene="yoaB"
                     /locus_tag="ACHJ1G_002453"
     CDS             24859..25203
                     /gene="yoaB"
                     /locus_tag="ACHJ1G_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4764299.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         6191 a         6464 c         6104 g         6582 t
ORIGIN      
        1 aataaaatcc agcactcaca ttgctctcct ttttatggtt tctatgggta cacaaaaatt
       61 aaaagctcaa agctttttta ttttcagttt attgctgacg ttaattttat tttgcatcac
      121 cacattatat aacgaaaaca caaatgtaaa actcatccca cagatgaatt acctgatggt
      181 tgttgtggct ttgtttttcc ttaacgccgt catttttctt ttcatgttaa tgaaatattt
      241 cactaacaaa caaattttac caacactcat tttaagcctt gcatttttaa gtggccttat
      301 ctatttagtt gaaaccattg taattatcca taaaccaatt aacggcagta cactgatcca
      361 gacaaagtcg aatgatgttt ctattttcta tattttccgc caactcagtt ttatttgttt
      421 aacctcgctg gcactctttt gttatggaaa agacaacatc cttgacaaca ataagaaaaa
      481 aacgggaatc ctgttgctgg cgctgattcc ttttttagtt tttccccttc tggcacacaa
      541 tctgagcagt tataacgctg actattcttt gtatgtcgtc gactactgtc cggacaacca
      601 tactgcgacc tggggaatca actatacaaa aatattggtt tgtctgtggg catttttact
      661 gttctttatt atcatgcgta cacgattagc cagcgaacta tggccgttaa tagcattatt
      721 atgtctggca tcgctatgct gcaacttact tctactgact ctggatgagt ataattacac
      781 catctggtat atcagtcgcg ggattgaagt ttccagtaaa ctgtttgttg tgtcttttct
      841 gatttataac atttttcagg aactgcaact ctccagcaaa ctggcagttc atgatgtgct
      901 gaccaatatt tataatcggc gctacttttt caacagcgta gagtcattat tgtcgcgacc
      961 tgttgttaag gacttctgtg tcatgctggt tgatattaat cagttcaaac gcatcaatgc
     1021 ccaatgggga catcgtgtgg gtgataaagt gctggtttca attgtcgata ttatccagca
     1081 aagcatccgc cctgatgata ttttagcgcg actggagggt gaggtgtttg gcttgctatt
     1141 taccgaactc aatagtgccc aggcaaaaat cattgcggaa cgtatgcgta aaaatgtcga
     1201 actcctgacc ggctttagta acagatatga tgttcctgaa caaatgacca tcagtattgg
     1261 cacggttttt tcaacgggtg acacgcgtaa tatctcgctt gtcatgacgg aagcagataa
     1321 agccttacgc gaagcgaaaa gcgagggggg caacaaagtg attattcatc atatttaagt
     1381 gcaaaaatat tcagagccat gctttttgcg tggcttttgc atacaattta ttactattac
     1441 ccctaaattt cccctcccca gcggcgcaga gatgagtata attagcgccc ctgtgccagg
     1501 ccgcaatcga actttatctg gttttctcgt ttcactaacc gaaggagtgc catttatcat
     1561 gaaattgcac catagaatgc tccggcattt tatcgccgca agtgtcattg tgctgacatc
     1621 ttccttcctt atttttgaac ttgtcgccag cgacagagca atgagtgcct atctgcgcta
     1681 tatcgtgcag aaagcagact cctcctttct ttatgataag tatcagaatc agagtattgc
     1741 cgcgcatgtg atgcgcgctc tcgctgctga gcagtcggaa gtgtcgccag aacagcggcg
     1801 cgccatctgc gaggcttttg agtctgccaa taatacccat ggcttaaacc tgactgccca
     1861 taaatacccg ggcttacgcg gcacactaca aaccgcatcc actgactgcg acacaattgt
     1921 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
     1981 tgattacggt tcaggtcttg ggatggccga agagaaattt cactattatc tcgatctcaa
     2041 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
     2101 gtccttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
     2161 cgggcgtacc gtattgtcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
     2221 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
     2281 caaaaacaat ttgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
     2341 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
     2401 taatctgttc cagtacgtca gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt
     2461 atccattgat attctttact ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt
     2521 gacggcgtta attttgctga atatggtccg gatgcacttc cgtttatacc aaaatgtgtc
     2581 gcgagaaaat attagtgatg cgatgactgg actgtataat cgcaaaattt taacccctga
     2641 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgcaat
     2701 tgacatggac aagttaaagc aaataaatga caccctcggt catcaggagg gggatttagc
     2761 gattacgtta ttagctcagg cgattaaaca atcgattcgt aaaagtgatt atgccatccg
     2821 actcggtggc gatgaattct gcatcattct tgtcgattcg acgccgcaaa ttgcagcaca
     2881 actgcctgaa cgtatcgaaa aacgtctgca acatatcgcg ccgcagaaag aaatcggctt
     2941 ctcttccggt atttacgcga tgaaagaaaa cgatacgtta catgatgcgt ataaagcttc
     3001 cgatgagcgt ttatatgtca ataagcagaa caaaaacagc cgttcatgat aaccttctgt
     3061 ggttgtttgc ttgtaatctc aggagcgtga aatgactgaa atggctaaag gaagcgtgac
     3121 gcatcagcgg ttaatcgctt tattatcaca agaaggtgct aacttccgcg tcgttaccca
     3181 tgaagcggta ggtaaatgtg aagcggtgtc agaaattcgt ggcaccgctc taggccaggg
     3241 tgctaaggca ctggtatgta aagtcaaagg caatggcgta aatcaacatg ttctggcgat
     3301 cctcgctgcc gatcaacagg ccgatctgag ccaactcgcc agccatatag gcggattacg
     3361 cgcttcgttg gccagcccgg cggaagtcga tgaactcaca ggctgtgtct tcggcgcgat
     3421 cccccctttc agcttccatc caaaactcaa actggttgcc gaccctctac tcttcgaacg
     3481 atttgatgaa atcgccttca atgcagggat gctggataaa tccgttattt tgaaaaccac
     3541 ggattatttg cgcattgcgc aaccagaact gctcaatttc cgccgcactg cgtaactagc
     3601 ctgtccgttc aataagcaga acggacaaaa ccagcacaac agcaatggca aaaaacgatg
     3661 acgtgataat cagtgtctcg acgaacattt gatcgttcat agcatgcccc cgggagatag
     3721 ctgttttcac gttattgtta gcgtgcacaa atggcagttt gatgacagtt cgcctgattt
     3781 tttatgcaaa aaacgtaaag attttctact tccttcctgc agcaagcgta aagtaagcag
     3841 gcttattatt ttttggcaag gaaaccacga tgtttgatgt cactttgctg atcctgctcg
     3901 ggttagctgc gctgggcttt atcagtcata acaccactgt cgccgtttca attctggtgt
     3961 tgatcattgt ccgcgtcaca ccgttaagca cctttttccc gtggattgaa aaacaggggc
     4021 taagtatcgg gataatcatt ctgacgattg gcgttatggc ccccatcgcc agcgggacgc
     4081 taccgccctc aaccttgatt cactcgtttc tgaactggaa atcgctggtg gcaattgcag
     4141 taggcgttat tgtctcctgg ctgggtgggc gcggcgtgac cttaatgggc agccagccgc
     4201 aactggtcgc cgggctgctg gtaggcactg ttttaggcgt ggcgctgttt cgcggcgtac
     4261 cggtcggccc gcttattgcc gcgggtctgg tttcgctgat tgtggggaaa cagtagttaa
     4321 tcccgcaata tatcggcctg gcgtttgccc caggcctttc ttaaacatag taataaaggc
     4381 cgttgttgaa tcatacccca gcgtatgagc aactttctgt acagtatcgc ctttcactaa
     4441 tccctgaagt gccataatca gctgtaactg ctgacgccac tggcgaaaac tcaaccccgt
     4501 ctcttttact atcagtcgcg caagattgcg ttcactcatc gcaaaaaagc cagcccattg
     4561 ccctaatgac ccccactcga cgggcccttt cgccatcatc tccaccatcg tgcggatttt
     4621 aggatgagaa gaaacgggca ggtgcagctg ttgttgtggc tgctgaggga gttcatcaaa
     4681 caggacctga attaatctgc gggtcatcgg ctcagcacgc tgagtcgtcg tcctgtttgc
     4741 cagggttaaa atcaattccc ggcataaggg cgatattttc aatgtgcaac atgttgtcgg
     4801 cattgtcact gcgctgggtt cgataaacag aaaacagagt tcagcgttag ctgtcacttg
     4861 attgctatgt tccaccccgc ccggtatcca aaccgcatat tgcggcggca ccatccacaa
     4921 agcattttcc accgtacagg taattgcacc atgtaatgct agtatcagct gtcctttgcg
     4981 gtgctgatgt acaggactgg ttaactcatc cgttccggcg tgaatacaaa atgcgaccgc
     5041 ggcttcatgg tgtcggtcgg gttcatagcc attgagattc aacctgtgca tcattttgtc
     5101 cgaatttagc gataatttgt cattttagct tgattcaaca taacaataaa aacggtaagg
     5161 tacagcctcg tttgtaacaa tgagaagcat atgacctgtt caacttcatt aagcggcaaa
     5221 aacaggattg tccttatcgc tggcattctg atgattgcca ctacattacg cgtcaccttc
     5281 accggcgcag caccgttact cgatacgatt cgttccgctt actcgctgac gacagcgcaa
     5341 accggcttat taaccaccct gccattattg gcctttgcgc taatctcacc tttggctgcc
     5401 ccggtggcgc gacgttttgg tatggaacgt agcctgtttg ccgcgttact tttgatctgt
     5461 gcaggtatcg caattcgttc tctcccttcg ccttacttat tatttggcgg tacagcggtc
     5521 attggcggtg ggattgcatt aggcaatgtc ttactgccag gattaattaa acgcgatttc
     5581 cctcattccg tcgccagact taccggcgca tattccctga caatgggagc tgcagcggca
     5641 ctgggatcgg ctatggtcgt gccgctggct ttgaacggtt ttggctggca aggcgcgttg
     5701 ttcatgctga tgtgttttcc tctgctggct ctttttttat ggctgccaca gtggcgaagc
     5761 caacaacatg caaatttgag tacctcgcgc gccttacata ctcggggtat ctggcgttcg
     5821 ccgcttgcct ggcaggtcac attgtttctt gggatcaact cactggtcta ttacgtgatt
     5881 attggctggc ttccggcgat cctcatcagt cgcggctata gcgaagcaca ggcgggttca
     5941 ctgcatggtt tgctgcaact agccacagca gctcccggtt tgctgatccc tcttttctta
     6001 catcatgtga aagatcagcg tggtattgca gcgttcgttg ccttgatgtg cgcagtgggc
     6061 gctgttgggc tctgctttat gccagcgcac gcgatcacct ggactctgct tttcggtttt
     6121 ggttccggcg caacaatgat actggggttg acgttcattg gtcttcgggc aagttctgcg
     6181 catcaggcgg cggcactctc ggggatggca caatccgtcg ggtatttgct ggcagcctgt
     6241 gggccgccgc tgatgggtaa aatacacgat gctaacggtg actggtccgt accactgatg
     6301 ggtgttgcca tactttcact actgatggcg attttcggac tttgcgccgg gagagacaaa
     6361 gaaattcgct aatatccggt gctatagtga cgtgacaaat catgcgtgaa agggagaaca
     6421 aacacgatga atattcagtg caaacgcgtt tatgatccgg ctgaacagag cgatggttat
     6481 cgcgtactgg tcgaccgcct ctggccgcgc ggtatcaaaa aaaccgattt agcccttgat
     6541 gagtgggata aagaaatcac gccgtcaacg gaactgcgca aagcctttca cggcgaagtc
     6601 gtcgattttg caacctttcg cgagcaatat cttgcagaac tggcgcaaca cgagcaagaa
     6661 ggaaagcggc tggcggacat cgccaaaaaa cagccgctga ccctgctcta cgcagcaaaa
     6721 aacaccacgc agaaccatgc gctggtgctg gccgactggc tacgtagctt gtgattttag
     6781 tacagcatcc ggcggttatt tttcaccagc cggatggtcc cgtcgccaca atgcccattc
     6841 atcaatcgtt tcaccgcccg gtaatttgca attgttgctg accccttgcg ctgtctgcac
     6901 tggaatgagc gtcccgccct tctgctggca atagaccgac gccggatttg ccataccaat
     6961 ctgcggcggt ttaggtgctt ctggctgaga agaggttgaa caaccagcca ggaccagcaa
     7021 gcaaggcaaa gcaaaactga taattttcat ttattgatct cacacattta tctacgctta
     7081 gagtatcgcg gtatcgtttt gttttgcagc actattttta ttacattcac tcaaaacata
     7141 ttacgtcttg tttcatcttt gttgatgatg ttttatcatg cctgcaaaga ttaaataatc
     7201 agcatttacc cgccgtatcc tggagttgtt tcgtgtcaga tcagattatc gcccgcgtct
     7261 cgcaatccct tgccaaagaa cagtcactag aaagtctggt ccgacagctt ctggagatgc
     7321 tggaaatggt cactgatatg gaatcaacct acctgaccaa agtggatgtc gaagcgcgcc
     7381 tgcagcatat tatgtttgcc cgtaacagcc agaaaatgca catcccggag aattttaccg
     7441 tctcgtggga ttactcgtta tgcaaacgcg ctattgatga aaactgcttt ttcagcgatg
     7501 aagtccctga ccgttggggc gactgtattg cggcacgcaa tcttggaatc accacatttc
     7561 tgagtacacc aattcactta ccggatggat cattctatgg cacgctttgc gccgccagca
     7621 gtgagaagcg ccagtggagt gaacgcgcgg aacaggtttt acagttattc gccggactga
     7681 ttgcacaata tattcaaaaa gaggcgttgg ttgaacagct gcgcgaagcc aatgccgcgc
     7741 tgattgcgca atcgtatacc gactcgttaa ccgggctacc gaatcggcgg gcgatttttg
     7801 aaaatctgac gacgctgttt tctctcgccc ggcatcttaa ccataagata atgatcgcgt
     7861 ttatcgatct ggataacttc aaattaatca atgatcgttt tggtcataat agtggcgatc
     7921 tgtttctcat tcaggttggc gagcgcctta atacgctcca gcaaaatggc gaagttattg
     7981 gtcgtctcgg cggtgatgag tttttggttg tttcactaaa taacgagaat gcggatattt
     8041 cgtcgctgcg agaacgtatt caacagcaaa tacgtggaga atatcactta ggtgatgttg
     8101 atttgtatta tcctggtgcc agtcttggca ttgtagaagt cgatcctgaa acgaccgatg
     8161 cagacagtgc cctgcatgct gccgatatcg cgatgtatca ggagaaaaaa cacaaacaga
     8221 aaacaccttt tgtcgcgcat tcagcgccac attcctgagg cgtattcaca tccttttgat
     8281 tggtgataac atgcgaatcg gtattatttt tccggttgta atctttatta cagcggtcgt
     8341 atttttagca tggtttttta ttggcggcta tgctgccccg ggagcataaa gatgaaaaaa
     8401 acaacgatta ttatgatggg tgtggcgatt attgtcgtac tcggcactga gctgggatgg
     8461 tggtaacgtc acctctaaaa aatagcaaag gctgcctgtg tgcagccttt gtgcaattta
     8521 agcgttaact tttaatcttc ctgtagataa atagcacgac aatcgcacca ataacggcaa
     8581 ccacgaagct gccaaaattg aagccatcga ctttaccaaa gccaaacagc gtgctgatcc
     8641 atccgccaac tacggcaccg actatcccca gcaggatagt cataaagaat ccaccaccat
     8701 ctttacctgg catgatccac ttcgccagaa taccggcaat aagcccaaaa ataatccatg
     8761 acagaatgcc cattgtttcc tcacttatct gttttgcatt agcgggttag tcgctgataa
     8821 aaagcatagc acaacatcgg gagggcaaga tttgtgacga gcatcacgga ggattttttt
     8881 gcggatggca cagaaattgc gccatcaacg atcagtgata attaccaacc acaaacatca
     8941 tgttcgtttt ccgtgtcttg acatcctcca cgccctgaat gacgaggatc cctgctacgt
     9001 tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc aacgggcggc ctgactgcac
     9061 catctctcca caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccgtttac
     9121 atcggcgttc gctgtatatc cacatacctg gcatctgaat tgactttgtg acaggcgatt
     9181 ttctttcgct gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc
     9241 aagcacctga ccgccactcc agagctgctt gtactcaagc tggcggcgca tttcatacca
     9301 gccctgatcc agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg
     9361 gctgaccgta cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat
     9421 cattgcgtgg tttttgctga cggtcgttgt gactttatga aggtagtccc tgcggatatt
     9481 tgcgatacgg gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tattgctgaa
     9541 tttgaccttg cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa
     9601 actgtttaca ggcccaaaga ctgtgccatc tgacagcgtg gcgagtttag ccacgccagc
     9661 atccagcccg atcattgatg ctgaagggtg aaccggagtt gatacttcac tttctgtctg
     9721 aatactgatg taccacttac cgcaggactg gctgaccgtg acatttttca caacacccgt
     9781 gacctgacgg ctattccggt agcgcatcca gcccagtttc ggcagaaaaa tacggctgtt
     9841 ttcctgatcg agctttacgc cctgcgggta gcggaatgca tcattctgtc cccgcttttt
     9901 gaatcgggga aaagcagccc gcttccggaa gaagtttttg taagcccgct caaggtcttt
     9961 cagtgactgt tgcaatggct gtgagggcga atctttaagc cattgcgttt cagtggcgtt
    10021 tttccactca accagccagg aagccatttt cccgtaaggg atgtatttat taccggcctc
    10081 atgattctca ttctgacgtg ccagagcacg attgaaaacg aaacgacatg cgcctgcgaa
    10141 gcgcctcatt tcacactcct gttgaccacc tggtcttaac tggaatttaa atgcctgtaa
    10201 tcgtttcaca ggtcaaatga tactttggtc tgtgaaaaaa gaaaccgata tccggcgtgg
    10261 aaggcattgt gttttcctga tgcatgttca cctggtcttt gtcaccagat accgactcca
    10321 gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctga
    10381 ttttgaagct gaacttgttg aaatggatgg cgaaccagat cacgtccatt tgttaattaa
    10441 ctatcctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag
    10501 gttactgcga cgagatcgac cagatattgc agtcaggtat tactacaaag gcgttttgtg
    10561 gagtcctggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    10621 cattgaacaa cagcaaacac ctggttagat ggaaaaccgc gccttatatc cccgccctga
    10681 aggacggggt tttacggcgc accggataag aacgcacggt attcaccaga tcttttatca
    10741 cttcagccgc cacttctggc accagcaaag tcatcggcgt ctctgtttca taatcgacag
    10801 aaacgccatt gctgttattg gtgacggtca cggtatacgt tgctttgccc atgattcatt
    10861 tcccgttatg aatgactttc cgttgttgcg caccttccat caggacttca ggagccacga
    10921 agaagtcaat gttgaaataa gtatcgtcag tcatggcttc aatgttgtgc cacttttctg
    10981 gagggaacac cgcaaactgc cccgcttcga taaggatcac ctgatcaggc tctgcactgt
    11041 gttcatcagc gtagccgaga tatttgaccg ccccatgcat aacggaaagg cgtgggtaaa
    11101 cccccgggcg cgttccttta tcaagatgac gttcgaatat tccggcaggc gcagtttgtt
    11161 tattccagaa aggcgttgag cgcgtatgaa tataattctg tgggatttgc agcatcctat
    11221 tccctccttc ggggaatgcg ctgaaaacgg tttattccag ccgtttcagg gtacgcctga
    11281 taatttgcat tttaaatacc atttattggt tactttttag caccatatca gagaaaaatc
    11341 agggaggatt acaagtgggg agcccgcgac aacgcgggct gaatgtatca ggattgcagc
    11401 gtcgccagtc gggcggcgaa acccacgaac atcaaaccaa tcagtgagtt gccaacttta
    11461 gccagtttct ttttggtacg tatgtactgc gtgacaaaag caccagaaat aatcaggaag
    11521 ctcaaatagc agaaactcac cagttccagc gtcgtcgcca gaataaagaa tgaaattccc
    11581 gtatgtgggg cattaacatc gataaactgt acgaaaaacg acacatagaa caaaatggct
    11641 ttcggattag tcaggctcaa aattaacgcg cgtttaaaaa tggccccgta ttggggctca
    11701 tcggatttgg cctcgttatt tttacccttc agggtcgcgt agagaatttt actccccaga
    11761 tagagcaaat aaaacgcacc aagataacgt acaatgttga ataatatcgg ggtggtctta
    11821 attaatgtcg ccactccagc ccatgccaga aacatcaata ctgcatcgcc aataaataca
    11881 ccgcaggccg caagataacc gcctttcata ccgctactga cgctattttt gagtacaaac
    11941 agggtatttg gccctggcac caacacaata aaaatggccc caaccagata ggtccagtaa
    12001 ttcagaaccc cgtattcagc gaacacatta acctctttaa ttatctttcg atcatgcgcg
    12061 attaaaggtg aatatgctaa ccaatctgta gcggcttaga aaggagaaaa tcaggtttta
    12121 acccgatatc aacccgataa ttgaatcatt aatcaggcat gctccagtga aaaattcggg
    12181 tagtgctcgg caaaatactg gcgtaaaaat tctaccgtta tccgcacttt cgctgacgtc
    12241 gccagccttg aaacataaac ggcccagacg ttcgctggct ggtaatattc cggtagcact
    12301 tgcactaaat gaccactggc aatgttttcg ctaacatccc accaggagcg cagagcaatc
    12361 ccctgcccat cgagacacca ctgatggaca atttctccat gattagacga cagtgggccg
    12421 gtgactttaa tcgcatggtg gccttcttta ttgcgtaatt gccagacacc aaaaggatga
    12481 tcgcgctctt taatgaccag gcacggcaat gcggaaagat cggttaaatg tttcggcgcg
    12541 ccatgttgcg caataaattc cggcgacgcg cagagaatgc ggtaattagt cgccagtttg
    12601 cgggcgatta aattaggggc gatgtcatcg ccaatgcgaa tatcgagatc aacaccttca
    12661 ttgaccaaat cgaccaaccg gtcttccaca tcaaaacgta attccagttg cggatacgcc
    12721 ttagccagcg ctgataacgc cggagccacc acctgtcgtc caaaaccaaa gctgctgata
    12781 atacgcagca tcccctgcgg cacctgacgc acgtcagaaa gttcgtccat catctgaccg
    12841 acatcctgca aaatccgctg cgcccattca taaatccgct ctccttcttc ggtaatggtg
    12901 acgcggcggg tggtgcggtg taacaacacc acgtttagcg tttgctccag caaggcgatg
    12961 cgcttgctga cgaacgccgg tgaaacgccc agttcttccg ccacggcggc aaaaccagcc
    13021 cgacgagcca ccagcataaa gacgcgcaaa tcattcagca gcggtaaatt attcatgatt
    13081 cgtgttttat gtttcaccag ttacggggat taattccttt tcagtcaatt ataggatggt
    13141 gatgttgtca attttgatgg tcaggaagtg agaacccaat gatgaaaacg atgcgtattg
    13201 ctgcgatccc gggagacggg attggcaaag aagtccttcc tgaagggatt cgcgtgttac
    13261 aggctgccgc tgagcgctgg ggcttcgcct tgagttttga gcaaatggag tgggcgagct
    13321 gcgagtatta cagccatcac ggtaaaatga tgccggacga ctggcatgag caacttagcc
    13381 gtttcgacgc catctatttt ggtgccgtcg gctggccgga caccgttcct gaccatattt
    13441 cgttgtgggg ttcgctgctg aaatttcgtc gtgaattcga ccagtacgtc aacctgcgcc
    13501 cggttcgtct ctttcctggc gttccctgcc cgctggcggg aaaacagcct ggcgacatcg
    13561 atttttacgt ggtcagggaa aacaccgaag gcgaatattc ctcgctcggc ggtagagtga
    13621 atgaaggtac agagcatgaa gtcgtcattc aggaatcggt atttacgcgt cgtggtgtcg
    13681 atcgcatttt gcgttatgcc ttcgaacttg cgcaaagccg cccacgtaag acgctaactt
    13741 ctgccactaa atcaaacggt ttagccatca gcatgccgta ctgggatgag cgagtggaag
    13801 caatggccga gaattacccg gagatccgct gggacaagca gcatattgat attctctgcg
    13861 cgcgttttgt gatgcagccg gaacgcttcg atgtggtggt ggcgtccaat ttatttggcg
    13921 atatcctttc cgatcttggc ccggcctgca ccggcaccat tggcattgcc ccatccgcca
    13981 acctgaatcc ggaacgcact ttcccgtcgc tcttcgagcc agtccacggt tccgcgccgg
    14041 atatctacgg gaaaaacatt gctaacccta tcgccacgat ttgggccggt gcaatgatgc
    14101 tcgattttct cggcaatggc gatgagcgtt tccagcaagc gcataacggt attctggcag
    14161 caattgaaga agtgattgct cacgggccga aaacacctga tatgaaaggc aatgccacca
    14221 cgccacaggt tgccgacgcg atttgcaaaa ttattttgcg ttaaggtcaa accagtttat
    14281 ttgaaccgcg tcactgacgc ggttttttta ttcgttcttt gcagtaaata acctgcgtca
    14341 tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt ccgtattctt aattgtttaa
    14401 tttatgtaac atgcaaataa tcaatttgtt aaacacaaca tacaactcat taccctgcaa
    14461 ataattacaa ctggcaaaag ccagagacgg gcttttatgc ccaggagatg actcatgatg
    14521 agcaatgtta agaaaaaaga tgtgccgctg ataagcatca gcctggtggc cattcttttc
    14581 atcgcagctg cattaagcct tttcccacaa caatcggccg acgcggccaa cgcaatatac
    14641 acttttgtta cgcgtacgtt aggttccgcc gtacaggtat tggttttgct ggcaatggga
    14701 ctggtgattt atttagccac cagtaaatac ggcaatattc gtcttggcga aggaaaaccg
    14761 gaatacagca cgctctcctg gctgtttatg tttatttgtg ccggtttagg ttcttctacg
    14821 ctttattggg gggttgctga atgggcctat tattatcaaa cgcctggatt aaatatcgca
    14881 ccgcgttcac aacaggcact cgaatttagc gtcccttact ccttcttcca ctggggcatc
    14941 agcgcctggg caacttatac gctggcctca ttaatcatgg cttatcactt tcatgtgcgt
    15001 aaaaacaaag gtctgagtct ttccggcatt attgccgcga ttaccggcgt tcgcccgcaa
    15061 ggcccatggg gaaaactggt tgatttgatg ttcctgatcg ccactgtcgg cgcactgact
    15121 atttcccttg ttgtcaccgc agcaaccttt actcgtggac tttccgcgct gaccggttta
    15181 cccgataact tcaccgtgca ggcatttgtg atcctgcttt ccggcggcat tttttgccta
    15241 agctcgtgga ttggtatcaa caacggtttg caacgtctga gcaaaatggt tggctggggc
    15301 gcgttcctgc tgccattact ggtgctgatt gtcggcccaa ccgaatttat taccaacagc
    15361 atcatcaatg ccatcggtct gaccacgcaa aacttcctgc aaatgagctt attcaccgat
    15421 ccgcttggcg atggttcatt tacccgcaac tggaccgttt tctactggct gtggtggatc
    15481 tcatacaccc ctggcgtagc aatgtttgtc acccgcgttt cccgcggtcg taagattaaa
    15541 gaagttatct ggggactgat cctcggcagc accgtcggtt gctggttctt cttcggcgta
    15601 atggaaagct atgccattca tcagtttatc aatggcgtaa tcaacgtccc acaggtgctg
    15661 gaaacactgg gcggcgaaac agctgtacag caagttctga tgtcgttgcc agccggtaaa
    15721 ttgttcctcg ccgcatacct gggcgtgatg attattttcc ttgcctcgca tatggatgcg
    15781 gtggcctaca ccatggctgc gaccagtacg cgtaatctcc aggaaggtga cgatcctgac
    15841 cgtgggctgc gtcttttctg gtgcgtggtg atcactctga tcccgctttc catcttgttt
    15901 accggtgctt cgctggaaac gatgaaaacc accgtcgtgc tcacagccct tcccttcctc
    15961 gtcattttac tggtgaaagt cggcggattt attcgctggc tgaaacagga ttacgccgac
    16021 attccggctc atcaagttga acattatctc ccgcagacac cggttgaagc cctggaaaaa
    16081 acgccagtgc tccctgcggg aaccgtattc aaaggcgaca actgagcgcc atcatcctaa
    16141 cgataaaggt atccctatga gcaatctgag ccctgacttt gtactacccg aaaatttttg
    16201 cgctaacccg caagaggcgt ggaccattcc tgcccgtttt tataccgatc agaacgcgtt
    16261 tgaacacgaa aaagagaacg tcttcgccaa aagctggatt tgcgtcgctc acagcattga
    16321 actggcgaat gccaatgatt atgtgacgcg tgagatcatt ggcgaaagca tcgtgctggt
    16381 acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg tgtccgcacc gtggtcatca
    16441 gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc tgcccgtatc acgcatgggc
    16501 attcaaactc gatggcaatc tggcccatgc acgtaactgc gaaaacgtcg ccaatttcga
    16561 tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa tatgccggat tcgtcttcat
    16621 caacatggac cccaacgcca ccagcgtaga agatcagtta cccggtctgg gcgcgaaagt
    16681 gctggaagcc tgcccggaag tccacgatct gaaactggcg gcccgcttta ccacccgcac
    16741 gcctgccaac tggaagaaca ttgtcgataa ctatctcgag tgctatcact gtggtccggc
    16801 gcatccaggt ttctccgact ccgtacaggt tgatcgttac tggcacacca tgcacggtaa
    16861 ctggacgctg caatacggtt tcgccaaacc gtccgaacag tcgtttaaat ttgaagaggg
    16921 tacggatgcg gcattccacg gtttctggct gtggccgtgc acgatgctga acgtcacccc
    16981 gatcaaaggg atgatgacgg tcatttatga attcccggtg gattctgaaa ctaccctgca
    17041 aaactacgat atttacttca ccaatgaaga gttaaccgac gagcaaaaat cgctgattga
    17101 gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg gttgaaagcg tacagaaagg
    17161 gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg gccgacagta gcggtagtgg
    17221 catttccgaa catggtatcg cccatttcca taatctgctg gcgcaggtgt ttaaggacta
    17281 atgacatcgg cggcggtatt ttccgccgct gggctgattt ttgatggagt acagcaatgt
    17341 cagactatca aatgtttgaa gtacaggtga gtcaggtgga accgcttacc gaacaggtga
    17401 aacgcttcac gctggtggca accgatggca aaccgctgcc tgcgttcacc ggaggaagtc
    17461 acatcattgt gcagatgagc gatggtgata accagtacag caatgcgtat tcactactga
    17521 gttcgccgca tgacacctct tgttatcaga ttgccgttcg gctggaggaa aactcgcgcg
    17581 gcggttcccg ctttttgcat cagcaggtaa aagtgggcga tcggttaacg atttcaacgc
    17641 ctaataacct gtttgcgcta attccctcag ccagaaagca tctgtttatc gcgggcggta
    17701 ttggtatcac ccctttcctg tcgcacatgg cagagctgca acacagcgac gtcgactggc
    17761 agctacatta ctgctcgcga aatccagaaa gttgcgcatt tcgtgatgag ctagtccagc
    17821 atccgcaggc tgagaaagtt cacttgcatc actcatcaac cggaacacga ctggaattag
    17881 cgcgattatt ggcggatatc gaacctggca cacacgttta tacctgtggt cccgaggcgc
    17941 taaatgaagc ggtaagaagt gaagctgcgc gtctggacat cgtcgccgat acgctgcact
    18001 ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcctt taccctggtg cttgcccgtt
    18061 ccggaaaaga gtttgtggtg ccggaagaga tgactatttt gcaggttatt gaaaataata
    18121 aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg cggaacctgc gaaacagcaa
    18181 tactggaagg tgaagctgac catcgggatc agtattttag cgatgaagag cgtgccagcc
    18241 agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa acgcctggtg ttggatttgt
    18301 agtttgccaa agccggatgt ggcgctgagc gcgcccagtc cggcttcgga agattttact
    18361 gcggatattc ctgcaataaa ttgtgtaatg cttccgccat cagctcacca cgccagccgg
    18421 aaatcagcgc cggcaaattg ttctgcggtt tcagtttcca gtgccagttc agcagttggt
    18481 tgatttgccg acgcgatgcc agcaattcgg cgctgatctt atgcgtttcg ctcacgtcag
    18541 taatcagcga cttaatcgct ttaaacactt tacgataacc aggcatgtcc atcaggttaa
    18601 gcatcggctg cggtaaggcc tcttccggca atgtctgcgc tttttccacc agcgccagca
    18661 gcgttttacc gtgaaagcgg atttcgctac cggataaacc caggctgtcc agttcgccta
    18721 aacttcccgg catataacgc gcgaccgacc acaaatgctc ttcacgcacg acaaagttca
    18781 ccgccagatc acgctctcgc gccttgcgca gtcgccagtc ggctaacagt tgcagacagg
    18841 ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc acgccaggca tcttccggcg
    18901 caacgacttc ctgacgacgc atttgcatca ggcggcattc atccagcgcc gcaggtagcc
    18961 agccggaggc ctccgtttct accataagct tggcggtgat cggtaacaga taccagacat
    19021 ccgccgctgc gtattcacac tgacgttcgg tcagcggtct ggccagccag tcggtgcgcg
    19081 attcactctt gtccagcgta acgccggaat actcttccac catggaagcg aaaccccatg
    19141 acatcgggcg tccgcagaag gcagcaagga tttgcgtgtc aatcaagggt tgtggtaatt
    19201 cgccaaagac attgaggaac acttccagat cttcactgcc tgcatggaga aattttgtga
    19261 tggacggatc gcgcaggatc gctttcagcg gtgaccagtc ggtgatcccg agaggatcaa
    19321 ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc cagctgcggg taataagtgc
    19381 gcgtacgaac aaattcagta tccagggcta tcgccggaaa ggcacggacg gcttcacaca
    19441 aagaagccag cgcatcgtcc gtggtaatca tttggtaatt caaattgttt tctctttagt
    19501 gggcgtcaaa aaaaacgccg gattaaccgg cgtctgacga ctgacttaac actcaggctt
    19561 tattgtccac tttgccgcgc gcttcgtcac gtaattctcg tcgcaaaatt tttccgacgt
    19621 tagatttcgg taactcatca cgaaactcca ccagcttcgg tactttgtag cccgtgagct
    19681 gacggcggca aaaggtcacc agtgactctt cggtaagcga tggatctttt ttcactacga
    19741 agattttcac cgcttcacca ctggagccgg aaggtacgcc aacagccgcg acttcctgta
    19801 cgccaggatg ctgcatgacg acatcttcaa tctcgttggg atagacgtta aaaccggaaa
    19861 ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa cccttcttca tccatcaccg
    19921 cgatgtcgcc ggtgtgtaac cagccatttt tgatgatctc atctgtagca tccggacgct
    19981 gccagtaacc cagcatcacc tgcggtcctt tgacacaaag ctcacccggt tgacccggtg
    20041 gtacttcatt atcatcatca tccaccagtt tggcttccgt cgacggcacc ggcaaaccga
    20101 tgctaccact atgataatca atatcatatg ggttaacgct gaccagcggc gcacactcgg
    20161 taaggccata gccttccagc agatactgtc ctgtcagttt cacccaacgc tctgccacca
    20221 cttgctgcac tggcatccct ccgcctgcgg aaagatgcag actggagaaa tccagctgct
    20281 ggaactcttt attgttcagc aacgcattga acaaggtgtt aacgcccgtg atagcggtaa
    20341 acggatattt cgctaactct tttaccaacc ctggaatatc gcgcgggtta gtgataagca
    20401 ggttctgccc acccagttcg ataaacagca ggcagttaat ggtcagggca aaaatgtgat
    20461 acagcggcag cgccgtcacc accagctctt tgcccggatg caacagcgga ccataggtcg
    20521 cgttaacctg ttccaggttc gccagcatat tgcggtgagt cagcatcgcg cctttcgcca
    20581 caccagtggt gccgccggtg tattgcagaa aagctaaatc ttccggcacc agttcgggtt
    20641 tgacgtactg catccggtag ccgttatgca gtgcgctacg aaatgaaatg gcatctggca
    20701 gatggtattt cggcaccaaa cgcttgatgt atttaacaac gaaattgact accgtgcctt
    20761 ttgccgtaga tagctgatcg cccatacggg tcagaattac gtgctgaacg gcggttttat
    20821 caaccacttt ttccagtgtg tgagcaaagt tagacacgat aacaatcgcc gatgcgccgc
    20881 tatcgttaag ctgatgctca agctcacgcg gggtatacaa cgggttaacg tttacgacga
    20941 tcatcccggc acgcaaaatg ccaaacagcg ccaccggata ttgcaataaa ttaggcatca
    21001 tcaacgcaac gcgatcgcct ttcttcagcc ccaacccttg ttgcaaataa gcggcaaacg
    21061 cgcgactgcg ttcttccagc ttgcggaagg tcattacctc ccccatattc acaaacgcag
    21121 gttgatcggc gtagcgcgcg accgactgct caaacatatc taccagagat tgataacggt
    21181 cagggttgat ctccgtcgga acgtccgcgg gataacggtt aagccaaacc ttcttcaatt
    21241 cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc aaatgatata catgccgtta
    21301 acataatatt aactcatcat accagcttga taattaccca acgaaaaggt tgcgaagcgc
    21361 gtcactattt atttttatct ttaccgtaag aatgcagaaa cagcggacca gccgctgttt
    21421 ctttttcttt aaaaacaagc aattattcag ttacaactgt ttgtactctc gcggggccgg
    21481 gattatacca gccccatccg ccgtagccat agggccagcc acgaccgcca taaaaccatg
    21541 gatcaatcgg ctgaggcggc ataatcacct gctgggttaa atgccaacgt ttgtaacccg
    21601 ttacttgcat caccataaat ttatagggcg tattgccgat tttgccgtca accgcaccag
    21661 tgattggccc gactaccgta acaagttgtc cacggaaatc caccgggtcc agaaaaccgt
    21721 tcacatcggc ataaatgcga ccgcgagaag gttctcccag cgtcggtctg gctccgctgt
    21781 ccagcggtac ggtagcaatt tccaggcggg ttttcccttg ctggttttgt accgcaacca
    21841 ctttgccacc aaagcgtgcc tcctgaccaa cgtacagctg cggcgcactc atcacccgaa
    21901 ctaaatcttg ttgcggcgtg ggactgctgc ctttaatggc gtccggcaca gtgacacaac
    21961 cgctcagcat tagcgcaaac gtacctgcca gtatgccttt gataacattc ttttgaaccg
    22021 ccatgatgcg actccttttt ttctcagggc atactcttaa gattcattct ttgcccggaa
    22081 gtttcttcca tgcgacgttg ttacgtaaat aaaccggttc ggcatgttcc accgccaccg
    22141 ttttaccctc ggcaaacatc tgacacgcaa tcggcagcat atcttccgcc gctggcagta
    22201 aaacttctcc gtcgcgcaaa accagcccgc tctctttacc gagatccggc caggcttgcc
    22261 agcccgtccc taccgttacc cattcgccgg aaagctgttg cattcgttca tgaacgagtt
    22321 cgggtttgag tacggcttcg gtttcttcac cgcgccagat accgttttca tcacgctgat
    22381 attcggccca gtaaacttcg cccattcgcg cgtcaatggc tgccagcacg cgggttgcgc
    22441 cgtttttgcg ccacgcccct tgcgccatcg tcattagcgt ggagacgcca atcatcggta
    22501 gctctgcgcc aagtgccagc ccttgcgcaa tgccaatacc aatgcgcacg ccggtaaagc
    22561 taccggggcc gcgcccgtaa accagagcgt taatatcagt cagggaagtt ccgctggtgg
    22621 tcaggatatc ctgcaccatc ggtaagattc gttgagtatg ttcacgaggg caaagctcaa
    22681 aatgagcgtt gacagtaccg tcgttccaca gggcgacaga gcaggcctct gtcgcggtat
    22741 cgatagccag aattcgcatg ggtcttcgtg cttagatcaa taaaaaggcg cgcatcatac
    22801 catactccgt aacaaattac ctggaggatg gtatcgcaag gaaacgaacc gcacgggcaa
    22861 tgtcacgagt gcgtggcgcg ggcggcagac tggcgagaaa cgtcgcgccg taaggacgca
    22921 tcaccagccg attgtcacaa atcaccagca cgccacgatc gtcggcgtcg cgaatcagtc
    22981 gccctacccc ctgtttgaga gtaatgacgg catctggtag ttgcacttca tcgaacgggt
    23041 cgccaccgcg caaacgacaa tcttccatgc gcgcttttaa cagtggatca tccggcgagg
    23101 taaacggcaa cttgtcgata atcaccaatg acaatgtatc gccacgcacg tccacccctt
    23161 cccagaaact actggttgcc acaagaagcg cgttaccggc gctgacaaat tgctgcaaca
    23221 gttgcccttt gctggtttcc ccctgcaaca aaacgggaag cgtcatggta gcgcggaact
    23281 gctcggccag atcacgcatc atggcgtgcg aggtacaaag cataaaacaa cgaccgttgt
    23341 tagcttcgat gatcggtcgc agcattgccg ctaactggcg agcagaacct ggctggttgg
    23401 tttgcggcag attgcgcggc acacagagta acgcctggcg gctgtaatca aacgggcttg
    23461 gcaatagcaa cgactccgcc tgttctatcc caagccgcga ggtgaaatga tgcagatcgt
    23521 cgttcaccga cagcgttgct gaggtgaaga tccagctacc aggcttttgc gccattaact
    23581 ctttgaattt atccgccacg ctgagcggcg tgagagccag agtaaaatgg cgcgaagtgc
    23641 attcgtacca gtagctgtag cccggctgat tgatctcttt tagccgcttc agccgtgtgc
    23701 gatacaacgt agcgcgctca aatgccgcat ccagcaaggc ggaacgcccc agtgacagtt
    23761 tcgccacgtc ataacaaagt tccagggtgt catcgagcag taaaaatgcc cgctgaattt
    23821 gcggattagc taacagctca cgcaggttac cgcgataacc tggctcaccg agttgcagac
    23881 gaaaatcctg cgcactctgg gcaagacgat cagcgcactt ttgtaactgc tgggtgtctt
    23941 ttaattcggt gcggtaagcg atggtgatgt cttttgccag gtcgagcagt tgtcgactgg
    24001 agagtgactg accaaaatac tggctggcaa tgtccggtag ctggtgggct tcgtcgaaga
    24061 tcatgacgtc cgcttccggg atcagttcgc caaatccact ctctttaacc accatatccg
    24121 ccagaaagag atgatggttt accaccacca catcggcgtc catcgctttt ttacgtgctt
    24181 tgaccacaaa gcaatcttta tacatcgggc agtcgctgcc aagacagttg tcgttggtgc
    24241 tggtgaccag cggccacgcc tgtgaatctt ccgccacgct gacgcaggtg ctgatatcac
    24301 catcgactgt ttgattagac caggagcgca gcaggatcac atcgcttaag atttgtaccg
    24361 gcagatcgcc ccccgccagc gcctgctgtt cgagacgctc gaggcagagg tagtttgagc
    24421 gccctttcag cagcgccacg ttgcccgtat atttcaatgc ctttgagact gttggcaaat
    24481 cgcggctgta gagctgatcc tgcaacgctt ttgagccggt cgagataatg actttctttt
    24541 tcgcccgcag cgcaggagcc aggtaagcgt aggttttgcc cgtaccggtt cctgcttcca
    24601 ccaccagcgg ctggcctttt tctatcgcct gggtgacggc taccgccatc tgtcgctgtg
    24661 gttctcgcgg cttaaagcct ggtatcgctt tcgccagctg accgtctggt gcaaaatcgt
    24721 ccgtcacact accccctgtt gatttgaaca gggattatgt caggatgagg gcgctttcgc
    24781 cagttgaatt ggtgacggcg acctcacatt gtggcagtct ttgcagcacg aaaatggaag
    24841 gataatgagg acaaaatgat gactatcgtt cgtatcgatg ctgaagcccg ctggtctgat
    24901 gtggtaatcc acaacaacac gctctactac actggtgtac cggaaaacct cgacgccgat
    24961 gcctttgagc aaaccgccaa cacgctggcg cagattgatg ccgtgctgga aaaacagggc
    25021 agcaataaat cgagcattct ggatgccacc attttcctgg ccgataaaaa cgacttcgcg
    25081 gcgatgaata aagcgtggga tgcctgggtt gtcgcgggtc atgcgccggt gcgctgcacg
    25141 gtacaagcgg gtttgatgaa cccgaagtat aaagttgaaa ttaagattgt ggctgcggtg
    25201 taagctttat cgaagcaaaa taagtcagac gataatttat cgataatact ggtcggtttt
    25261 acataaatcg accagagatt aagatattac ctgttgatgc tcctgctaat tcattattta
    25321 tttttataat ttcaatttta t
//
LOCUS       DAZFDP010000058        25334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFDP010000058 DAZFDP010000000
VERSION     DAZFDP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHJ1G_002454"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHJ1G_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4764300.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHJ1G_002455"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHJ1G_002455"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4764301.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHJ1G_002456"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHJ1G_002456"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4764302.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHJ1G_002457"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHJ1G_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4764303.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHJ1G_002458"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHJ1G_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4764304.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHJ1G_002459"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHJ1G_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4764305.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHJ1G_002460"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHJ1G_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4764306.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHJ1G_002461"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHJ1G_002461"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4764307.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHJ1G_002462"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHJ1G_002462"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4764308.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHJ1G_002463"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHJ1G_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4764309.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHJ1G_002464"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHJ1G_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4764310.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHJ1G_002465"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHJ1G_002465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4764311.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHJ1G_002466"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHJ1G_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4764312.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHJ1G_002467"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHJ1G_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4764313.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHJ1G_002468"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHJ1G_002468"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4764314.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHJ1G_002469"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHJ1G_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4764315.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHJ1G_002470"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHJ1G_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4764316.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHJ1G_002471"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHJ1G_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4764317.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHJ1G_002472"
     CDS             16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHJ1G_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4764318.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHJ1G_002473"
     CDS             17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHJ1G_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4764319.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHJ1G_002474"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHJ1G_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4764320.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="ACHJ1G_002475"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="ACHJ1G_002475"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4764321.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="ACHJ1G_002476"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="ACHJ1G_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4764322.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="ACHJ1G_002477"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="ACHJ1G_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4764323.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHJ1G_002478"
     CDS             complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHJ1G_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4764324.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHJ1G_002479"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHJ1G_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4764325.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="ACHJ1G_002480"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="ACHJ1G_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4764326.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHJ1G_002481"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHJ1G_002481"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24828..25076
                     /locus_tag="ACHJ1G_002482"
                     /pseudo
     CDS             24828..25076
                     /locus_tag="ACHJ1G_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            complement(25185..25334)
                     /locus_tag="ACHJ1G_002483"
                     /pseudo
     CDS             complement(25185..25334)
                     /locus_tag="ACHJ1G_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT         6465 a         6684 c         5940 g         6245 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catctgtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcgaaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccgcgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattact accccttaaa
    12721 acaccagcaa acagataaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
    15181 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
    15241 tagcgcgctc ttttatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctgat aattgccgcc ctgtacgcgc tacaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcgttt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tactaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacttattt agcatggaaa atatcgacaa ggccgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattatca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggtgcag tcatcctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    17881 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctactgtc tgtgctcgat ctggaaaaga tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
    18241 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    18541 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
    18601 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccccactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcacgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcctgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaatcagc ttgaagacgg gcatgaatgt ttgcttcgtc gagtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    20821 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tactgcagga
    20881 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccact tttgatagga ctgcaatcgg acagcggcaa
    24601 taaaaacagc cacttacgga ctctgaccag acagagcgca gaccgagaaa agacaaaaaa
    24661 atgcacgtgc actttaaaag aaccccagat ctcacggtct ggtttttttc tgtttgtaac
    24721 taatggtaac aaaaacacat cacctcttgg cgttccttct ccttgatact gtttatccat
    24781 acagttaaaa ataacactgt atacaaacac agtatagaga gatttttatg cgtattgaaa
    24841 tttgcatagc caaagaaaaa atcagcaaaa tgccaaaagg atctgttcct gccctacagc
    24901 aagaaatgct gtgacgtgcc agtaaacgtt atgacgatgt agaagtgatc atcaaatcca
    24961 ccagcaacga tggcctttca gttactctca ccgccgataa agattctgca aaaacttttg
    25021 ttcaggagac gctgaaagat acctgggagt ctgctgacga gtggtttgtt cgctaattaa
    25081 cgagtaaaat cagtaacggc tggaaatcat tcaatactca cactatctaa aagttcacca
    25141 gccaacagcg gtaagttctt tcatacgacg tgccgcggat cctattattc tagtatgaaa
    25201 tccctaaccg atttcaaatt ctccagactg ctcagattct cttttatccc acgctggcgc
    25261 tgataaatct agtcattgca atcgacctgc gctatcgctg ccgcagttct tccagtttct
    25321 gcatcgataa ctcc
//
LOCUS       DAZFDP010000059        25173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFDP010000059 DAZFDP010000000
VERSION     DAZFDP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..2083
                     /gene="yihQ"
                     /locus_tag="ACHJ1G_002484"
     CDS             47..2083
                     /gene="yihQ"
                     /locus_tag="ACHJ1G_002484"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO4764327.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2129..3514
                     /locus_tag="ACHJ1G_002485"
     CDS             2129..3514
                     /locus_tag="ACHJ1G_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764328.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3557..4960
                     /locus_tag="ACHJ1G_002486"
     CDS             3557..4960
                     /locus_tag="ACHJ1G_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764329.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5028..5720
                     /gene="ompL"
                     /locus_tag="ACHJ1G_002487"
     CDS             5028..5720
                     /gene="ompL"
                     /locus_tag="ACHJ1G_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO4764330.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5811..7076)
                     /locus_tag="ACHJ1G_002488"
     CDS             complement(5811..7076)
                     /locus_tag="ACHJ1G_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764331.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7178..8158)
                     /locus_tag="ACHJ1G_002489"
     CDS             complement(7178..8158)
                     /locus_tag="ACHJ1G_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO4764332.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8166..8876)
                     /locus_tag="ACHJ1G_002490"
     CDS             complement(8166..8876)
                     /locus_tag="ACHJ1G_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4764333.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9093..10916)
                     /gene="typA"
                     /locus_tag="ACHJ1G_002491"
     CDS             complement(9093..10916)
                     /gene="typA"
                     /locus_tag="ACHJ1G_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4764334.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11289..12698
                     /gene="glnA"
                     /locus_tag="ACHJ1G_002492"
     CDS             11289..12698
                     /gene="glnA"
                     /locus_tag="ACHJ1G_002492"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4764335.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13017..14066
                     /gene="glnL"
                     /locus_tag="ACHJ1G_002493"
     CDS             13017..14066
                     /gene="glnL"
                     /locus_tag="ACHJ1G_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4764336.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14078..15487
                     /gene="glnG"
                     /locus_tag="ACHJ1G_002494"
     CDS             14078..15487
                     /gene="glnG"
                     /locus_tag="ACHJ1G_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4764337.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15599..15709
                     /locus_tag="ACHJ1G_002495"
     CDS             15599..15709
                     /locus_tag="ACHJ1G_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4764338.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15899..17272)
                     /gene="hemN"
                     /locus_tag="ACHJ1G_002496"
     CDS             complement(15899..17272)
                     /gene="hemN"
                     /locus_tag="ACHJ1G_002496"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4764339.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17461..17970)
                     /gene="yihI"
                     /locus_tag="ACHJ1G_002497"
     CDS             complement(17461..17970)
                     /gene="yihI"
                     /locus_tag="ACHJ1G_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4764340.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(18089..18255)
                     /locus_tag="ACHJ1G_002498"
                     /pseudo
     CDS             complement(18089..18255)
                     /locus_tag="ACHJ1G_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18552..19184
                     /gene="yihA"
                     /locus_tag="ACHJ1G_002499"
     CDS             18552..19184
                     /gene="yihA"
                     /locus_tag="ACHJ1G_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4764341.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19566..22352)
                     /gene="polA"
                     /locus_tag="ACHJ1G_002500"
     CDS             complement(19566..22352)
                     /gene="polA"
                     /locus_tag="ACHJ1G_002500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4764342.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22716..23648
                     /locus_tag="ACHJ1G_002501"
     CDS             22716..23648
                     /locus_tag="ACHJ1G_002501"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO4764343.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(23689..25119)
                     /locus_tag="ACHJ1G_002502"
     CDS             complement(23689..25119)
                     /locus_tag="ACHJ1G_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4764344.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         6117 a         6323 c         6097 g         6636 t
ORIGIN      
        1 taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg
       61 tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc tacattttca
      121 acaacgtctt attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat
      181 agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat
      241 tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg
      301 tggttctgac attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct
      361 ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc
      421 agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc gtggcaaacc
      481 gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
      541 gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc
      601 tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt
      661 cgactttagt gccccggaat accatgaact ggcgctgtgg gaagacaaag caacgctgcg
      721 ttttgaatgt gctgacacat acatttccct gctggaaaaa ttaaccgccc tgctgggacg
      781 ccagccagaa ctgcccgact ggatttatga cggagtaacg ctcggcattc agggcgggac
      841 ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat
      901 ctgggcgcag gactggtccg gtattcgtat gacctctttt ggcaaacgcg tgatgtggaa
      961 ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca
     1021 ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata atgatctctg
     1081 cgaagaagcg gcacaacacg gctatctggc aaaagatgcc tctggcggtg actatctggt
     1141 ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg
     1201 gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga
     1261 cttcggcgag tatctgccca ccgacactta cctgcataac ggcatcagcg ccgaaattat
     1321 gcataacgcc tggcccgcgc tgtgggcgaa atgtaactac gaagcccttg aagaaacggg
     1381 caagctcggc gagatccttt tctttatgcg cgccggttct accggtagcc agaaatactc
     1441 caccatgatg tgggcgggcg accagaacgt cgactggagt ctcgacgatg gcctggcgtc
     1501 ggttgtcccg gcggcgctgt cgctggcaat gaccggacat ggcctgcacc acagcgacat
     1561 tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg
     1621 cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa
     1681 ctggcagttt gacggcgacg cagaaactat cgcccatttt gcccgtatga cctccgtctt
     1741 caccaccctg aaaccttacc tgaaagaggc tgtcgcgctg aatgcgaagt ccggcctgcc
     1801 ggttatgcgc ccgctgttcc tgcattacga agacgatgcg cacacttaca ccctgaaata
     1861 tcagtacctg ttaggtcgcg acattctggt cgctccggtg catgaagaag gccgtagcga
     1921 ctggacgctc tatctgccgg aggataactg ggtccacgcc tggacgggtg aagcgttccg
     1981 gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga
     2041 tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacatc cgcccgtggg
     2101 gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac
     2161 tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat
     2221 cctgctggat atcggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc
     2281 aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat
     2341 gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc
     2401 ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac
     2461 accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact
     2521 gtttttcagc atgatgaact gctcctacgg cgcaatggtt cccgctatca ccaaaaaccc
     2581 caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct
     2641 gtgcacggtg ggattcgtgc cagtgatgaa tcttatcgag ggtaatcagc aacttggcta
     2701 tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc
     2761 aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa agcctggcct
     2821 gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt gtattgccaa
     2881 cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta
     2941 cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt
     3001 catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat
     3061 cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc
     3121 ggtcagcttt gtggcgttct cctgcctggc gttcttcggc tcagcgtttg ttaacagcct
     3181 gaactgggcg ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc
     3241 ggaaggcacg gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg
     3301 tttcttcccc ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga
     3361 ccacaccatt gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt
     3421 caccattgtg gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt
     3481 ggaagagata gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc
     3541 gttataagga gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga
     3601 aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct
     3661 gtatctcctc aaattttata ccgatgaatt agggatgcct gcttactatg gcgggattat
     3721 ctttctggtg gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga
     3781 ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt
     3841 tccggcggcg ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa
     3901 aacgacgatt gccaccgcgc tgtttatgat gtttggactt tcatacagtc tgatgaactg
     3961 ctcgtatggt gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc
     4021 ggcttaccgc caggggggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc
     4081 gttgcagtcg cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc
     4141 cattggcggc tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga
     4201 cacagcgcca accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa
     4261 tccaccattg ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa
     4321 actggcgatt caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg
     4381 gatgggattc ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt
     4441 aaaatgtttt ggtaaaaaac aggtttattt agctggcatg gtgctatggg cggtgggtga
     4501 tatactgaat tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc
     4561 cttttttggc acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga
     4621 ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt
     4681 ctttcgtaaa atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat
     4741 tggttatgtt cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat
     4801 ttttatctgg ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac
     4861 actcaatgaa aaacggtttg cattgattat tgaggaaatc aaccaacgca aaaataaaga
     4921 gatggcgaca gaagaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc
     4981 catcctggtg ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta
     5041 atgcaataat tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg
     5101 aaaacaggga ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt
     5161 ataacttcga tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag
     5221 atgaactaaa acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg
     5281 ataaattaac catccagccc ggtggcttaa ttaatgataa gagtatcggt tcgggtggtg
     5341 cagtgtatct ggacgtcaac tataaatttg taccatggtt taatctgaca gtacgaaatc
     5401 gctataacca taataactat agttcaacag atttgagcgg ggaactggat aataatgaca
     5461 cctatgaaat tggcacctac tggaatttta aaatcaccga taaattttcc tatacatttg
     5521 agccacatta tttcatacgc gtaaatgact ttaatagcag caatggaaaa gatcatcact
     5581 gggaaatcac taataccttc cgttatcgca ttaatgaaca ctggctgcct tatttcgaat
     5641 tacgttggtt agatcgtaat gtcgaaccgt accaccgcga gcaaaaccag attcgtatcg
     5701 ggacgaagta tttcttctga tgtgaaactg ccggatgcgg cgtaaacgcc ttatccggcc
     5761 tacggttgat acggttttcg ttggccggat taagcacatc cggccattaa tcaagcttca
     5821 gccgccagca tctctttctt acgttggcta attcgcttaa ccagcattac ggaaaccacc
     5881 gcaccacaga atgcgaagca ggccataatg ccaaacacga ttttgtagcc aataatcccc
     5941 ggatttaaat ccagaatgta gccatacaga ctgaagcaga acatcgccgg agcgtaacca
     6001 ataaagctac ccaacgccat cgccgcgcct gttttatttt cagcaatttt tgcttcgccg
     6061 ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc cagcgtacat
     6121 gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag tacgagcgca
     6181 gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag gatcttatct
     6241 gaaatcatac caccaatcgg tccaccaatc attttcagac agtattggtt aatgatgccg
     6301 taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg aatgaagaat
     6361 gtcaggccac agtaaaccgc gtaaacgaag aagacgttaa aagcgataag ccagatggtt
     6421 ttgtctctca gcaccgaggt catactggtg ttttgcgatg ctccgtcttc ttttttcacc
     6481 tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat gcctacggca
     6541 atcacaataa gggaatagaa ccagatgcct gctttaaaac ctaataagcc actgccaaac
     6601 caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc acgccctgtt
     6661 tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact taccgacttg
     6721 agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca gacaaagagg
     6781 atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt
     6841 gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga cagaaaaaag
     6901 cccactgtgg tgacaaatga gtttaccgac atagcattac caatttgacc attggtcaaa
     6961 tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa tttataaatt
     7021 gtaccgccac acaatgttaa tagactaaat aacgcccatt tctttttcgt gagcataaca
     7081 tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga ccattcatat
     7141 ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga agatggtttg
     7201 cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta attgcatctt
     7261 ctttttcttt acgtaaccgc gcatccagta aatgattttc tggcaatggt gtttcactca
     7321 tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac gccggcgcat
     7381 agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca tcacccaggc
     7441 atataaggtg cgttaataac gctttgaacg gcatatcacc ctgacctgaa atacaggctt
     7501 tatgacccag gccacccggt tctttaacga tcaaggcatc tttgatatgg acctgggtaa
     7561 tatgcggcgc catcgttttt aaagcgtcga tgggatgctc atttgcattg atcatgttcg
     7621 caaaatcaaa cagtaaggaa agagattcca tctcactttc tttgaccagc gacaccagct
     7681 catggctttt taaatcttca tgctgttcga tcgtaaaagt caggccgctg tcctgatacg
     7741 tttcccgtac ataggcaatg tcgttagcga tgatcgataa tacgtcgcgc aaattacctt
     7801 cataacgtgg ataaaaacgt acggacgatg ccccagtttt caacgcaatg gcgacggctt
     7861 cgtcaataga tgccttatct gaagcgctgg tttcaatatg aatatcaagg ttcagacggc
     7921 gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga gaaaaacgct
     7981 cgccatccag aacatggatt ttgacgccac gtagattatt ttcacttgcg atatcaagta
     8041 aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa gcatgtaaat
     8101 aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt gtcaccataa
     8161 aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc aatatctatc
     8221 tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc
     8281 gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt gaaaatcaat
     8341 ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt
     8401 tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc gtgatagaaa
     8461 actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt tgatggtgtc
     8521 actgcaatca gtggcgcgag cgtcttggga atatcgctgg tacggttttt ctcggtaaac
     8581 ccccaggaag gctctcgtcc ttgctcaatt gcggcccgct gaaagctcgc cgacaactcc
     8641 ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt acggaagatt
     8701 ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt aatgcccagc
     8761 agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat gccatctttc
     8821 aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgattctc cgccattgtt
     8881 taatcctttt ggtttaaacc agatgaagca tcttacgctt acgccaaaaa agaaagcaat
     8941 taacggcgta tgactttgca gataattgtg acaaagctct ctattgacgt aatcccagac
     9001 ttataaggga gatactcgca ttggtgtgcg ctcacgacag gtaaaaaaaa acctgccagc
     9061 gatggcaggt tttttataac taaaaaacga aattaatcgt ctttcggtgc gcggttggcg
     9121 cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggagtcact
     9181 tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc
     9241 ggaaccagaa caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg
     9301 gtcaggcagt ttaccgtcag gtcgttagag cggctatgaa taccgataat ctgaccttcg
     9361 taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg
     9421 aacgcgaccg ctttaccctg accgttagag atcagtacgc cgttctgacg ctgacccact
     9481 tcacccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc agtaccggaa
     9541 gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat cacgtagtcg
     9601 agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc
     9661 agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac gttttcatac
     9721 ggctcttgtt tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt
     9781 tcgaaacctt cacgacgcat gttttcgatc agaacagaca ggtgcagttc gccacgacca
     9841 gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc
     9901 agttctttgt tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg
     9961 cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt
    10021 gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgcca
    10081 aggcccgtga tcgcaacgat atcgccagct tccgccagat cggtttcgat acgttccaga
    10141 ccgaggtggc ccagcacttt accgactttc gcgttgcggg ttttgccttc gctatcgatg
    10201 atagtgacct gctggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg
    10261 ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca
    10321 acgtccggcg caggaacgtg gtcaacaatc gcctggtaca gcggggtcat gtcttccgcc
    10381 atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag
    10441 tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc
    10501 caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg
    10561 taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca
    10621 accaccagca gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg
    10681 gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt
    10741 ttcgcgagga tggtaatccc ccgctctttc tccaaatcgt tggagtccat cacgcgctct
    10801 tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg
    10861 gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact
    10921 ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag
    10981 gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga
    11041 gcataaaaag ggttatccaa aggtcattgc accaacatgg tgcttaatgt ttccattgaa
    11101 gcactatatt ggtgcaacat tcacatcgtg gtgcagccct tttgcacgat ggtgcgcatg
    11161 ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc ttttttacgg
    11221 cgacacggcc aaaataattg cagatttcgt taccacgacg accatgacca atccaggaga
    11281 gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg
    11341 ttgatttgcg cttcaccgat actaaaggta aagaacagca cgtcactatc cctgctcatc
    11401 aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct
    11461 ggaaaggcat taacgagtcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg
    11521 acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa cctggcaccc
    11581 tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg cgccgaagat tacctgcgtt
    11641 ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc ctgttcgatg
    11701 acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat atcgaaggcg
    11761 catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag
    11821 gcggttactt cccggttcca ccggtagact cggctcagga tattcgttct gaaatgtgtc
    11881 tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta gcgactgctg
    11941 gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga
    12001 tctacaaata tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg acctttatgc
    12061 caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct ctgtctaaaa
    12121 acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag gcgctgtact
    12181 acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac ccgaccacca
    12241 actcttataa gcgtctggtc ccgggctatg aagcaccggt aatgctggct tactctgcgc
    12301 gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg
    12361 aagtacgttt cccggatccg gcagctaacc cgtacctgtg ctttgctgcc ctgctgatgg
    12421 ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac aaaaacctgt
    12481 atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag
    12541 cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg ttcactgacg
    12601 aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc
    12661 cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg tactacccgg
    12721 cgcaacaacg ccggaggttc aaaccaggcc catctggcgt aattgttgca gccagtttga
    12781 acacggacag cgcgcagaac ccggagcgta cactggtacg tgaggagttc gagcactgcc
    12841 caggttcaaa atggcaaata aaatagcctg atgggactgg tttttagttg ccgtggaaat
    12901 ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt cgcgcttttt
    12961 atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac tgctttatgg
    13021 caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac agtattttgt
    13081 taatcgatga caacctggcg atccattacg ccaaccctgc cgcgcaacaa ctgctcgccc
    13141 aaagctcccg caaattgttt ggtacaccgt taccggaact gttgagctac ttctcattaa
    13201 atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttaccgat aacgaagtga
    13261 cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt atgccggacg
    13321 gcatgatcct gctggagatg gctccgatgg ataaccagcg ccgcttaagt caggaacagc
    13381 tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca catgagatta
    13441 aaaatccgct tggcggttta cgtggcgcgg cgcagctgct cagcaaagcg ttacctgacc
    13501 catcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg
    13561 tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt taccgaaagt attcacaaag
    13621 tggctgaacg cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg cggttgattc
    13681 gtgattacga tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct
    13741 tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggaaggcggt gaaatcattc
    13801 tgcgtacccg caccgcgttt caactgacct tacacggcga gcgctaccgg ctggcggcgc
    13861 ggattgatgt ggaagataac gggccgggca ttccgcctca tttgcaggat acgctgtttt
    13921 acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc gctcgtaatt
    13981 tgattgatca gcattcaggc aaaattgaat ttaccagttg gccagggcat accgagttct
    14041 cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga tagtctgggt
    14101 agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg gggcaggttt
    14161 aacctgtacg acgtttgaga acggcgcaga agtgctggag gcgctggcga gcaaaacgcc
    14221 ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc tgctcaagca
    14281 gattaaacag cgccatccaa tgcttccggt catcattatg accgcacatt ccgatctgga
    14341 tgctgccgtc agcgcctatc aacaaggggc gtttgattat ctgcccaaac cgtttgatat
    14401 cgacgaagca gtggcgctgg ttgagcgcgc tatcagtcat taccaggaac agcagcagcc
    14461 gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc cagccatgca
    14521 ggacgtgttc cgtattatcg gtcggctttc gcgttcttct attagcgtgc tgattaacgg
    14581 cgaatccggc accggtaaag aactggtcgc tcatgccctg catcgccaca gtccgcgcgc
    14641 caaagcgccg tttatcgcgc tgaatatggc agctatccca aaagatttga tcgaatcaga
    14701 actgtttggc cacgagaaag gcgcgtttac tggcgcgaat accattcgtc aggggcgttt
    14761 tgaacaggcc gatggcggta cattattcct cgacgaaatt ggtgatatgc cgctggatgt
    14821 gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg gcggctatgc
    14881 gccggtgaaa gtggatgtgc ggattatcgc tgccactcac cagaatctcg aacagcgagt
    14941 gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg aacgttatcc gcgttcatct
    15001 gccgccgctg cgcgaacgtc gggaagatat tccccgtctg gcgcgccatt ttttacaggt
    15061 tgccgcgcgc gaactgggcg tagaagcgaa gttactgcat ccggaaaccg aagctgctct
    15121 gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc gctggctaac
    15181 ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cccggcgaac tgtttgaatc
    15241 aacggttgcg gagagtactt cgcaaatgca accggacagc tgggcgacgc ttcttgcgca
    15301 gtgggcagac agagcgctgc gttccggtca tcaaaatctg ctttccgaag cgcagccaga
    15361 gctggagcgg acgttactga cgaccgcgtt gcgacatacg caggggcata aacaggaagc
    15421 ggcgcggcta ctcggctggg gccgcaacac cctgacgcgt aagttaaaag agctggggat
    15481 ggagtgattc acagcttgtg tgtaaagatt gattattgag cgcaaattgc tggtatttta
    15541 cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga gagtcattat
    15601 gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca caccgcaaac
    15661 tgccgttgct gccgtgctgt gtgccgcaat gattgggctg tttagttgag tttcagattg
    15721 ctgatgaagt gctctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    15781 agcaatattt tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg caatcttgtt
    15841 ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca ttcactattt
    15901 aaatcacccg tgagaactgc tgcatccgcg ctttctggcg cagataggta tcaaagcaca
    15961 tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc ttctcatcca
    16021 catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc gcaaagtaat
    16081 cagcgaagtg caaatcccac agtttctcaa taggggcgta atccagacgg aagttgcaga
    16141 tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca ataccacgcc
    16201 acagcgcatt gccttgttca tccacttgct gatagtactg cttcaactct ttctggttct
    16261 gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc agatcggtat
    16321 cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc tgggccaccg
    16381 ccagctcgtc atccggacgg gcaaagtgat ccataccgat aaactgatag cccgattgcg
    16441 tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc ggcaggtcag
    16501 catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag ttaaagacac
    16561 tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa ctctccggcg
    16621 tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaaacca atctcacgcg
    16681 catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc agacgttgca
    16741 cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat
    16801 gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc tccgcatcgg
    16861 cattgaactg gaagttttcg cgcagcagct tcatcaggcg gctgatttgc gctttattca
    16921 gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg gcgaacagcg
    16981 gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc ttgtgctgct
    17041 ggcgagtaac aatcttattg caaccgcaga agtaacaaag cttatggcag aacgggatat
    17101 gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa aacgcctgtt
    17161 cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat
    17221 agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac atgctcactc
    17281 cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc gggtcatcag
    17341 ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga ataaccacac
    17401 cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg cggcaatccg
    17461 ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc
    17521 gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat ccagtttagc
    17581 atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac gttccagcag
    17641 cgcatccaga cgctcatccg tttccagtaa ttccaactcc gcctgcggtg aaagcatagg
    17701 tttctcactc ttcggtttgt gctgtttggt gactttttca gtcacgccca atggaatagg
    17761 ggttttactg ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc ctgacgtggt
    17821 gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct gacgcttgcg
    17881 atcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg catgaccttt
    17941 gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg ttttattcag
    18001 tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc
    18061 tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca
    18121 acacaccctg tttttccttt agccggaaca atccgtgttg attccatttc cgtttaatta
    18181 cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc
    18241 ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt
    18301 tgctttaaac aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa
    18361 agcaattcac tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt
    18421 cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg
    18481 ccgaaaaatc cgccttttat tatggttcag gtataatcgc cacaaagctt tgactgacgg
    18541 agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg
    18601 atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg
    18661 caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa
    18721 aaaccccagg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg
    18781 ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc
    18841 gtgcgctcgg cgaatacctc gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg
    18901 atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca
    18961 atatcgccgt tctggtgctg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag
    19021 cgcaattgaa tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg caggttgaaa
    19081 cgttttcttc gttgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt
    19141 ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct tgccttaatg
    19201 cttgtgccgg atgtggcgta tccggcccgt aaattcaata gattacaatt ctaccgttgg
    19261 cctgataaac atagcgcatc aggcattacg gatcttttct ttcgcccaat aaaaaacgcc
    19321 ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa
    19381 aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt actctttttt
    19441 gttcagttca gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat
    19501 agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc
    19561 gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca
    19621 tccagacggg tacagttttc catcagttga tgaatctgct tcgcgacggc atcaacatca
    19681 tctttatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt
    19741 tgctcagcct gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg
    19801 gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg
    19861 ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc
    19921 tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag
    19981 taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg
    20041 aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg
    20101 ctggtgacgg tttccagtgg caaactaaac acttctgccg ccgttgcccg gtggatatct
    20161 tttccttccg cgaatgcggt cagcaagcct ttgtcacgcg aaagatgcgc cataatgcgc
    20221 agttcaatct gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc
    20281 tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt
    20341 gacgataaac gtcccgttgc agttactgcc tggtgataag aggtatgcac acgcccggtt
    20401 ttcgggttga tcatcagcgg cagcttgtcg gtgtaggtcg atttcagctt cgccagacca
    20461 cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct
    20521 tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc ctgtttttca
    20581 aagagaatgg tttgcaactg cttggtggaa gaaaggttaa attcttcacc tgcaatttca
    20641 tgcgctttct tttccagctc agccagacgc aaggtgagct cttcagaatg attgtgcagc
    20701 actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg caccagcggc
    20761 atttcgatat tctcgaagac gttcaacggc cctttgtgtt tttgcagatc tggccacatt
    20821 ttcagatgca actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc ggcctcttcg
    20881 agggcaatct ggttaaaggt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg
    20941 atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg
    21001 ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg
    21061 taattcgcca gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca
    21121 tcttccagca gcggttttag caactcgagt gcacgctcgc gagagatttg atcgggcgcg
    21181 tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat agcaaaagaa
    21241 agcccgacca ggttagcaga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat
    21301 accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatggtg
    21361 acgtagttgt cataagaaat caccgttgcc gtcacttctg gcgcttcgtc tgcaacactg
    21421 gtttcctgcg gcttcgcggc tggttttgcc cctttggcct gtaaccattt gcccgcttcg
    21481 acatcagcag tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct
    21541 gccggttgct gcacttccag ttgttcacag gtcagctcca gttcaacgtc ggttttaatc
    21601 gtcgccagct ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt
    21661 gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc
    21721 agcccgccaa gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca
    21781 ggaatgttat cggaggagtc acccatcagc gccaggaaat cgatgatcag ttctggcggc
    21841 acgccgtact tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtattgata
    21901 agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgccagtgct gatcagcacc
    21961 ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg
    22021 ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcag gggttcgatt
    22081 tgtgctcgca gatcgttcgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca
    22141 tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc
    22201 atgatcagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg
    22261 ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca
    22321 aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg
    22381 ggtaaaggat gccacagaca ggatgaaaac atgagttttt caccgctttt cgtgaaaatc
    22441 ttttgtagat cttctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta
    22501 tttacatcgc ttttaattta ttatcatgct ttttacagca tgatatttgt ctttgttttt
    22561 catgcagtta aaaataaagc catccctaat aattgagata atgggatttt ttctaatgtg
    22621 gatagatatg aattattttt ctccttaagg atcatccgtt atttgggtcg ttttctgcta
    22681 aaatctgcgc cttgcgcttc tttagcgcac tgtctatggc taaccttttg aataaattca
    22741 ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg
    22801 tcactatttt ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg
    22861 tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg
    22921 aaggtctggc ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg
    22981 aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata
    23041 ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc
    23101 aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta
    23161 tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg
    23221 aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg
    23281 ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact
    23341 tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt aaacaattcg
    23401 ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata
    23461 tgttaagcgg taaactgacg cgaattgtcg ttcatgtgga tttacagcct attgccgatg
    23521 agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga
    23581 actcactgtg gcaggaaaaa gaccaacttc tgacgtcgtt gatgtcatca cagcggcagg
    23641 acaagtaaat tttgctcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa
    23701 acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt
    23761 acccggtgta taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa
    23821 catctgacct aaggtcatca tcccgctgat ttgtttatca gcccgctttt gcgctttttc
    23881 cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg
    23941 taacttcaca tcaaaattga gcgatttaat atctttgttt gtggatgatg aagatttggc
    24001 tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccattt
    24061 taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtaaatact ctttgaacaa
    24121 tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag
    24181 ctgcttctgc attgcaatgt tatactggat gataaattgg cgcaccgctg agggatcaat
    24241 agattcagca atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt
    24301 atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcat
    24361 ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga taatgttaat
    24421 ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgcgccag
    24481 agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat
    24541 atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt
    24601 aaactggccc tcaccaaaag aaaactttcc atactctgca ggggcaacat ctaatgtcgt
    24661 agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc
    24721 ggcgataaat agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag
    24781 tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc
    24841 cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcgaa
    24901 tgtcatttgc atttgaaaac gggaattaaa aaagcctttg tcaaagtttt tataacttaa
    24961 cgtcatatcg taggcatgtt cacctttctt tttcgcatcg ttaaaatctt taataaactt
    25021 ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc
    25081 taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac
    25141 agcaaaactt tgaatatcca cttatgctga aat
//
LOCUS       DAZFDP010000060        25126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFDP010000060 DAZFDP010000000
VERSION     DAZFDP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHJ1G_002503"
     CDS             complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHJ1G_002503"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4764345.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            1877..1999
                     /locus_tag="ACHJ1G_002504"
     CDS             1877..1999
                     /locus_tag="ACHJ1G_002504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764346.1"
                     /translation="MIDEKKSGREDLHRLSLLDATLKRRIRHKADYFLISYSGV"
     gene            complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="ACHJ1G_002505"
     CDS             complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="ACHJ1G_002505"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4764347.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4248..5048
                     /gene="nagB"
                     /locus_tag="ACHJ1G_002506"
     CDS             4248..5048
                     /gene="nagB"
                     /locus_tag="ACHJ1G_002506"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4764348.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5108..6256
                     /gene="nagA"
                     /locus_tag="ACHJ1G_002507"
     CDS             5108..6256
                     /gene="nagA"
                     /locus_tag="ACHJ1G_002507"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4764349.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6265..7485
                     /gene="nagC"
                     /locus_tag="ACHJ1G_002508"
                     /gene_synonym="nagR"
     CDS             6265..7485
                     /gene="nagC"
                     /locus_tag="ACHJ1G_002508"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4764350.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7533..8285
                     /gene="nagD"
                     /locus_tag="ACHJ1G_002509"
     CDS             7533..8285
                     /gene="nagD"
                     /locus_tag="ACHJ1G_002509"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4764351.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8542..10206
                     /gene="asnB"
                     /locus_tag="ACHJ1G_002510"
     CDS             8542..10206
                     /gene="asnB"
                     /locus_tag="ACHJ1G_002510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4764352.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10587..10663
                     /locus_tag="ACHJ1G_002511"
     tRNA            10587..10663
                     /locus_tag="ACHJ1G_002511"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10621..10623,aa:Met,seq:cat)
     gene            10712..10786
                     /locus_tag="ACHJ1G_002512"
     tRNA            10712..10786
                     /locus_tag="ACHJ1G_002512"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10744..10746,aa:Gln,seq:ctg)
     gene            complement(10940..12115)
                     /gene="ubiF"
                     /locus_tag="ACHJ1G_002513"
     CDS             complement(10940..12115)
                     /gene="ubiF"
                     /locus_tag="ACHJ1G_002513"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4764353.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12261..13685
                     /gene="miaB"
                     /locus_tag="ACHJ1G_002514"
     CDS             12261..13685
                     /gene="miaB"
                     /locus_tag="ACHJ1G_002514"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4764354.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            13838..14878
                     /locus_tag="ACHJ1G_002515"
     CDS             13838..14878
                     /locus_tag="ACHJ1G_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4764355.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            14875..15342
                     /gene="ybeY"
                     /locus_tag="ACHJ1G_002516"
     CDS             14875..15342
                     /gene="ybeY"
                     /locus_tag="ACHJ1G_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4764356.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15432..16310
                     /gene="corC"
                     /locus_tag="ACHJ1G_002517"
     CDS             15432..16310
                     /gene="corC"
                     /locus_tag="ACHJ1G_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4764357.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16335..17873
                     /gene="lnt"
                     /locus_tag="ACHJ1G_002518"
     CDS             16335..17873
                     /gene="lnt"
                     /locus_tag="ACHJ1G_002518"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4764358.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(17951..19828)
                     /locus_tag="ACHJ1G_002519"
     CDS             complement(17951..19828)
                     /locus_tag="ACHJ1G_002519"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO4764359.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20283..21191
                     /gene="gltI"
                     /locus_tag="ACHJ1G_002520"
     CDS             20283..21191
                     /gene="gltI"
                     /locus_tag="ACHJ1G_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4764360.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21361..22101
                     /gene="gltJ"
                     /locus_tag="ACHJ1G_002521"
     CDS             21361..22101
                     /gene="gltJ"
                     /locus_tag="ACHJ1G_002521"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4764361.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22101..22775
                     /gene="gltK"
                     /locus_tag="ACHJ1G_002522"
     CDS             22101..22775
                     /gene="gltK"
                     /locus_tag="ACHJ1G_002522"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4764362.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            22775..23500
                     /gene="gltL"
                     /locus_tag="ACHJ1G_002523"
     CDS             22775..23500
                     /gene="gltL"
                     /locus_tag="ACHJ1G_002523"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4764363.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            23618..24553
                     /gene="rihA"
                     /locus_tag="ACHJ1G_002524"
     CDS             23618..24553
                     /gene="rihA"
                     /locus_tag="ACHJ1G_002524"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4764364.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            24637..>25126
                     /gene="hscC"
                     /locus_tag="ACHJ1G_002525"
     CDS             24637..>25126
                     /gene="hscC"
                     /locus_tag="ACHJ1G_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4764365.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQ"
BASE COUNT         6165 a         6545 c         6397 g         6019 t
ORIGIN      
        1 gaaaagcaga aaaaacgccg cagagtgcgg cgtttgggat tgtttgctat ttcttgccgg
       61 atgcgacgta aatgcctcac ccggcctggt agaacttaaa attactcgcc tactttcgcc
      121 caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgctgt ggaatggcgg
      181 ctgtcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct
      241 ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac
      301 aggaaatcat ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt
      361 tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca
      421 tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg
      481 ccttcggcat ctttctccac gcgttcagcc ttaatgacgt aagcattacg cagacgcact
      541 tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga
      601 tcgatccaaa tctcaccgct aaacggcacc tgacgactcc ccatttccgg tttgttcgga
      661 tggttcggca tggtgaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc
      721 ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat
      781 tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac
      841 tcacgaatag aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc
      901 gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac
      961 atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg
     1021 gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt cctggaactc aagcgtacac
     1081 agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg
     1141 tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatacg gtacagcacc
     1201 ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta
     1261 ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg
     1321 cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc
     1381 tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag
     1441 gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct
     1501 aaccactcta cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta
     1561 gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg
     1621 cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg
     1681 tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta
     1741 gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg
     1801 gcgtatgatc ttataagcgg gacaggctga caactgttag ttttttaaaa ttgataatct
     1861 aacaaggatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt
     1921 gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat
     1981 ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact
     2041 gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt tagcgttcag
     2101 gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcac cctcttccac
     2161 cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat
     2221 ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat
     2281 tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc
     2341 caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt
     2401 aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac
     2461 aggcgcggca gttgccggag ttgcttcagc tgacgcagcg gctaccggac cacgggcaac
     2521 gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt
     2581 ctgtttgttc agtttcacta caccagaagc acccagacgt ttacacatcg tatcgttgac
     2641 gcgggcagag tcagccacgg tcaggcgcag acgggtgata caggcgtcaa ttgctttcag
     2701 gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc
     2761 agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt
     2821 tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac
     2881 aacacccata accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa
     2941 cgcgtagtcg atagcccccg ctgagaaaga gaagccagca tggataccca gcagtgttgc
     3001 cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa
     3061 catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga
     3121 aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag
     3181 cgccgcaccc ggcagaccga acatcataat cgggaagaag ccggacatga acatccccgc
     3241 ggtgccgtca ccggcataga agcggttgat atcaccgtgg aaaaccgtac ccgccgcgtt
     3301 ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg
     3361 gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat
     3421 ccactcgcct cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc
     3481 cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc cgccgaagaa
     3541 actcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag
     3601 accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc
     3661 tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt
     3721 cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc
     3781 gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag
     3841 tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa
     3901 acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta
     3961 tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg
     4021 ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt
     4081 ttgtcaccaa atatcgttat catcactccc ttttactggc taaagcagaa aacttatttt
     4141 atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa
     4201 gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc
     4261 ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca
     4321 atgcgttcaa accgactgcc gatcgtccgt ttgtactggg cctgccgact ggcggcacgc
     4381 cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc
     4441 acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct
     4501 actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca
     4561 accttctcaa cggcaatgcc ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa
     4621 tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg
     4681 catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc ctgactcatg
     4741 acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg
     4801 ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg
     4861 gtagccagaa agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga
     4921 ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca
     4981 tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag
     5041 gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg
     5101 ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga
     5161 ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc
     5221 gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt
     5281 gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga
     5341 aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac
     5401 gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct
     5461 ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt
     5521 aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt
     5581 cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc
     5641 ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc
     5701 gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt acctttgcca cccatctgta
     5761 caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga
     5821 agctgacatt tattgcggta ttattgctga tggcctgcat gttgattacg ccaacattcg
     5881 taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc
     5941 aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact
     6001 ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg
     6061 taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta
     6121 tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc
     6181 caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga
     6241 ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt
     6301 gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca
     6361 atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt
     6421 acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg
     6481 ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg
     6541 cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa
     6601 gaacattacc cgctgccgga acgtacccag caaacgctgg aacatgccct gttgaatgcc
     6661 attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc gatttcggtg
     6721 atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag
     6781 gtagaaaact gggggctggt agaagcactg gaagaacgtt ttaaagtgac ctgtttcgtt
     6841 ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc
     6901 gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg
     6961 cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg
     7021 ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc
     7081 gctattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg
     7141 gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg
     7201 gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta
     7261 tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc
     7321 cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg
     7381 gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc
     7441 gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg
     7501 gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata
     7561 tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg
     7621 ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc
     7681 aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc gtgttttata
     7741 cctctgcgat ggcgactgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg
     7801 tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga
     7861 accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag
     7921 cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc
     7981 gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca
     8041 aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcggcatta aacaaaatgc
     8101 aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgcaccgac attctggcgg
     8161 gcttccaggc gggtctggag acgattctgg tgctttctgg tgtttcatcg ctcgacgata
     8221 tcgacagtat gccgttccgc ccaagctgga tttacccgtc ggtcgctgaa atcgacgtta
     8281 tctgaatagc aaccacgtct cagggcgtgg tttcttattt ttacccatca ttaaaatcat
     8341 cccattcaat aaaactcgcc aaaaattatc gatccatcaa taaatacatc accacaccct
     8401 cccctttttc acaaatcagc aatcaccatt acgtttttta tttttgcact gccaaatcgc
     8461 ttgcgcacgg tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat
     8521 accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
     8581 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
     8641 gactggtccg gtatttatgc cagcgataac gccattctcg cccacgaacg tctgtcaatt
     8701 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggcg
     8761 gtaaacggtg aaatctacaa ccaccaggca ctgcgcgccg aatatggcga tcgttatcag
     8821 ttccagaccg gatctgactg cgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa
     8881 tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgca
     8941 tatctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgagcac
     9001 ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc cagtttgccg cacgattaaa
     9061 gagttcccgg cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat
     9121 cgcgactggt tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt
     9181 caggcactgg aagattccgt taaaagccat ctgatgtctg acgttcctta cggtgtgctg
     9241 ctctcgggtg gtctggattc ctcaattatt tccgcgatca ccaagaaata cgccgcccgt
     9301 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc aattacactc ctttgctgta
     9361 ggtctgccgg gttcaccgga tctgaaagca gcccaggaag tggcaaacca tctgggcacg
     9421 gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt
     9481 taccacatcg aaacctatga tgtgacaact attcgcgctt caacaccgat gtatttaatg
     9541 tcgcgtaaga tcaaggcaat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa
     9601 gtgtttggcg gttatcttta cttccacaaa gcaccgaatg ccaaagaact gcatgaagag
     9661 acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgcgccaa caaagcgatg
     9721 tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg
     9781 atgcgcatta acccgcagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
     9841 cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct ggcggcagaa agagcagttc
     9901 tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt
     9961 tctgatcagc aactggaaac tgcccgcttc cgcttcccgt acaacacgcc aacctcaaaa
    10021 gaagcgtatc tgtaccggga gatctttgaa gaactgttcc cgcttccgag cgccgctgag
    10081 tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag
    10141 gcgttcaaga aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat
    10201 aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct
    10261 tgttaattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac
    10321 tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa
    10381 tttcatcctg tacgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat
    10441 aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat
    10501 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    10561 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    10621 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga
    10681 atacgttcgg caaattcaaa aaccaatttg ttggggtatc gccaagcggt aaggcaccgg
    10741 attctgattc cggcattccg aggttcgaat cctcgtaccc cagccacatt aaaaaagctc
    10801 gcttcggcga gctttttgct tttctgcgtt cattcaatgt cgaatgcgat gttgacacgt
    10861 cttatccttc aatgtcggat gcgacgctgc cgcgtcttat ccgacctacg gttggcacgc
    10921 atccggcaat gttgtaaggc tacaacccta acgcatattt cagcgcctga cgtttcaaca
    10981 cgccagcacg ctctgccgcc attaacccga gattacgcac aaaacgcagc ggcggcagat
    11041 tattactgaa tccggcataa aacagatcca taccactttg cataatgaag ttatccgcca
    11101 tgcgccgcat ctggtaacgc ttgaggacag gataactggc ccacgcttca ccgtagctgc
    11161 gggcattcac cagaacatca atcagggcat cgacatcacg ataaccaaga ttccccccct
    11221 gccccgccag cggatggatg gtatgcgcgg catcgcccac cagcgcaagc cccggctgca
    11281 catactgcaa cgcatgtcgg cgcgtcagcg gaaacgcacc agcggcaagc ggtgtaacgt
    11341 aacccagacg cgacgggaaa tgcttcgcga tttccgcctg gagttgcgcc atattcatat
    11401 tctgcaactg gcgaatacgt accggagagt cataccacac cagcgatgcc cagttatcaa
    11461 acaacggcag aaacgcacgc ggtccgtccg gggtaaattg ctgccaggtg ctgtcgccgg
    11521 gatcgttctc acactgtacg ctaatcaaca tacacgactg cgcatactgc caggcatgaa
    11581 cgccaattcc cgccatctgc cgcacctgcg aatttgcgcc gtcagcacca atcaccagct
    11641 tcgcgcgaat cacttcaccg cccttcagtt ccagctcctg aagatcatta tctcgatgta
    11701 atgcaatcag cgagcctggc acacgtaacg ttactttcgg atgcgcttcc agcgcctgcc
    11761 acaatgcctg ttgcaggacg gtgttttcca ccatatagcc aagcagcggc agcttaagtt
    11821 cagcggcgtc aaacatcaca tgcgccgttt cccactccca cgtttccagt ctgcggtaag
    11881 gatggcaacg catagcctgt actgcatccc agacccctaa ccctttaagc aatgataccg
    11941 atgcggcgct gatcgccgag atccgcacgt ccggttggct atcagcgaca aacggtgcag
    12001 gttctgcgtg ctcgatcacc gttaccgaaa atccgtgctg tgccagcccc agcgccagtg
    12061 cgccgccgac cattcctccg ccgacaatgg caatttccgt tggttgattt gtcatggtca
    12121 atcatcctgt tagcaatatg ctttaagttt acaggatttt tacccccaag ggctgataac
    12181 gctcatactg gtcacaacgg cagcaaagca ttacactatg cgccctgcat tcctggctac
    12241 tatttcgcaa gagcaagtcg atgaccaaaa aactccatat taaaacctgg ggctgtcaga
    12301 tgaacgagta cgattcatcg aagatggccg atctgctgga tgccacccac ggctatcaac
    12361 tgaccgacgt ggcggaagaa gcggatgtgc tgctgctgaa cacctgctca atccgcgaga
    12421 aggctcagga aaaagtcttc catcagttgg gtcgctggaa actgttaaaa gagaagaatc
    12481 cagacctgat tatcggcgtc ggtggctgcg tggcatcgca agaaggcgag cacattcgcc
    12541 agcgcgccca ctatgtcgat attatttttg ggccgcaaac gctgcaccgt ctgccggaga
    12601 tgatcaactc cgtgcgcggc gaccgcagcc cggttgtaga tatcagcttc ccggaaatcg
    12661 agaagtttga ccgtctgccg gaaccgcgcg ccgaagggcc gaccgcgttt gtctccatca
    12721 tggaaggctg caataaatat tgcacctact gcgtggtgcc ttacacccgt ggtgaagagg
    12781 taagccgtcc gtccgacgat attctgtttg agatcgccca gcttgcggct cagggcgtgc
    12841 gtgaagtcaa cctgctcggt cagaatgtga atgcctggcg tggtgagaac tacgacggca
    12901 ccaccggatc gtttgccgat ctgttgcgtc tggttgctgc gatcgacggg atcgatcgta
    12961 ttcgctttac caccagccat ccgatcgaat tcaccgacga tatcatcgaa gtgtatcgcg
    13021 acacgccgga gctggtgagc ttcctgcatc tgccggtaca gagcggttcc gatcgcattc
    13081 tgaacctgat gggccgtact catacggcac tggagtacaa agcgatcatc cgtaaactgc
    13141 gtgcggcgcg tccggatatt cagatcagtt ctgacttcat tgttggcttc cctggcgaaa
    13201 ccaccgaaga cttcgagaaa acgatgaagc tgattgccga cgtcaatttc gacatgagct
    13261 acagctttat cttctctgca cgtccgggta caccagccgc cgatatggtt gatgatgttc
    13321 cggaagaaga gaagaagcaa cgtctgtata ttctgcaaga acgtatcaat cagcaagcga
    13381 tggcatggag ccgccggatg ctcggcacca cccagcgtat tctggtagaa gggacttcgc
    13441 gtaagagcat catggagctt tccgggcgta ccgaaaataa ccgcgtggtc aacttcgagg
    13501 gcacaccgga tatgatcggt aaattcgtcg atgtagaaat taccgacgtc tacccgaact
    13561 ctctgcgcgg taaagtggtg cgtactgaag atgaaatggg tctgcgcgtg gcagaaacac
    13621 cggaatcggt gattgcccgt acccgcaaag aaaacgacct tggcgtgggt tattatcagc
    13681 cgtaattctc aggcccgccg ttccggtggg ccttgttttt tctctcttta tccccatctt
    13741 tatcgcaatg cttgcgcctt tttcctgtgg cgtgaataat ttccttatag gccggtgaat
    13801 gttcagcttc cggcgacata cagataagag gaacggtttg aacatagaca ctcgcgaaat
    13861 caccctggag ccagcagaca acgcgcgtct gttgagcctg tgcggcccgt ttgatgacaa
    13921 catcaagcag ctcgaacgcc gtctcggcat cgagatcaat cgccgcgaca accactttaa
    13981 actgaccggc cgtccgattt gcgtcaccgc tgcggcagac attctgcgta gcctgtatgt
    14041 cgatacagcc ccgatgcgcg gtcagattca ggatatcgaa ccggaacaga tccaccttgc
    14101 gattaaagaa gcgcgagtac tggagcaaag tgcggagagc gtgccggagt acggcaaagc
    14161 ggtcaatatc aaaaccaaac gcggcgtaat taagccgcgc acgccaaacc aggcgcagta
    14221 catcgccaat attctcgacc atgacattac cttcggcgtt ggcccggcgg gtacgggtaa
    14281 aacctacctg gcagtggctg cggcagttga tgccctggag cgtcaggaga ttcgccgtat
    14341 tctgctgact cgtccggcgg tcgaagccgg tgagaaactg ggcttcctgc ctggcgattt
    14401 aagccagaaa gtagacccgt atctgcgccc gctgtacgac gcgctgtttg aaatgctggg
    14461 ctttgagaaa gttgagaaac tgattgagcg caacgttatt gaagtcgcgc cgctggccta
    14521 tatgcgtggt cgtacgctga acgacgcgtt tatcattctc gatgagagcc agaacaccac
    14581 catcgaacag atgaagatgt tcctgacccg tatcggtttt aactcaaaag cggttatcac
    14641 cggcgacgtc acacagatcg acctgccgcg taatactaaa tcaggcttac gtcacgccat
    14701 cgaagtgctg gccgatgtcg aagagatcag ctttaacttc ttccacagcg aagacgtggt
    14761 tcgtcacccg gtggtggcgc gtatcgttaa cgcctatgaa gcctgggaag aagccgaaca
    14821 aaaacgaaaa gcggcgctgg cggcagaacg caagcgcgaa gaacaggaac aaaaatgagt
    14881 caggtgatcc tcgatttaca actggcatgt gaagataatt ccgggttacc ggaagagagc
    14941 cagtttcaaa catggctgaa tgcggtgatc ccgcagtttc aggaagaatc ggaagtgact
    15001 attcgcgtgg tcgataccgc cgaaagccac agtctgaatc tgacctatcg cggtaaggat
    15061 aagccgacca acgtgctctc cttcccgttt gaagtgccgc ctggcatgga aatgtcgcta
    15121 ctgggcgatc tggttatctg ccgtcaggtg gttgagaagg aagctcagga gcaaggcaaa
    15181 ccactggagg cgcactgggc gcatatggtg gtgcacggca gtctgcattt gttaggttac
    15241 gatcacatcg aagatgacga agcagaagaa atggaagccc tcgaaacaga gattatgctt
    15301 gctctgggct atgaggatcc gtacattgcc gagaaagaat aatttgccag gcatttgact
    15361 ggcagacgtc cccgccgttg gtgattaacg acggcgtttt gattaactaa catgagaacc
    15421 catacgacgc catgagcgac gacaattcac acagtagtga cacgataagc aacaagaagg
    15481 gatttttctc cctgttactc agccaacttt tccacggtga accgaaaaac cgtgacgaac
    15541 tgctggcgct gatccgtgat tccgggcaga acgaccttat cgacgaagat acgcgcgata
    15601 tgctcgaagg ggtgatggac atcgcagacc aacgcgttcg cgacatcatg atcccccgct
    15661 cccagatgat taccctgaaa cgcaaccaga cgctggacga atgccttgat gtcatcatcg
    15721 agtccgccca ctcacgtttc ccggtgatta gcgaagacaa agatcacatt gaagggattc
    15781 tgatggcgaa agacttgcta ccgtttatgc gcagcgatgc tgaagccttc agcatggaca
    15841 aagtgttacg tcaggcggtt gtcgttcctg aaagtaagcg cgtagaccgg atgctgaaag
    15901 agtttcgctc tcagcgttac cacatggcga tcgttattga cgaattcggt ggggtttccg
    15961 gtctggtgac cattgaagac atcctggaac tgattgttgg tgagattgaa gacgagtatg
    16021 acgaagaaga tgatatcgac ttccgtcagc tgagtcgtca tacctggacc gtgcgcgcac
    16081 tggcttccat tgaagacttc aacgaagcgt tcggcaccca ctttagcgat gaagaagtcg
    16141 acactatcgg tggtctggtg atgcaggcgt ttgggcatct tccggcgcgt ggcgaaacta
    16201 tcgacatcga cggttaccag ttcaaagtgg cgatggccga tagtcggcgt attattcagg
    16261 ttcatgtcaa aatcccggat gactcacccc agccaaagct ggatgaataa aaccgaaact
    16321 ggatagataa ctacatggct tttgcctcat taattgaacg ccagcgcatt cgcctgctgc
    16381 tggcgttatt attcggtgcc tgcggaacgc tggccttctc tccttacgat gtctggcctg
    16441 cggcgattgt ttcgctgatg gggcttcagg cgctgacctt taaccgccgc ccgctccagt
    16501 ctgccgctat tggcttctgc tggggatttg gcctctttgg caccggtatt aactgggtct
    16561 atgtcagcat cgcgaccttt ggcgggatgc ctggcccggt taacatcttc ctggtggtgc
    16621 tgctggcggc gtatttgtcg ctgtataccg gactgtttgc tggcgtgctg tcgcgcctgt
    16681 ggccgaaaac cacctggctg cgcgtggcga ttgccgcccc tgctctctgg caagtgaccg
    16741 agtttctgcg cggttgggtg ctgactggct tcccgtggtt acagttcggc tatagccaga
    16801 ttgatggccc gttaaaaggg ctggcaccaa taatgggcgt ggaagccatt aacttcctgc
    16861 tgatgatggt tagcggcctg ctggcactgg cgttagtcaa acgcaactgg cgtccgctgg
    16921 tggtggccgt cgtgctgttt gcccttccct tcccgctgcg ttacatccaa tggtttaccc
    16981 cgcaaccgga gaaaaccatt caggtttcga tggttcaggg cgatattccg caatcactga
    17041 aatgggacgg agaccagctt cttaatacgc tgaagattta ctacaacgca acggcaccgc
    17101 tgctgggcaa atcatcgttg attatctggc cggagtcggc gataaccgat ctggaaatta
    17161 atcagcaacc gttcctcaaa gcactggacg gtgagttgcg tgataaaggt agctcgctgg
    17221 taaccgggat tgtcgacgcg cgtctcaata agcagaaccg ctacgatacc tacaacacca
    17281 tcatcacgct gggtaaaggt gcaccgtaca gctacgaatc agccgatcgc tataacaaaa
    17341 accatctggt gccgtttggc gagtttgtcc cgctggagtc gattctgcgt ccgttagcac
    17401 cgttctttga tctgccgatg tcgtcgttca gccgtgggcc atatatccag ccgccgctgt
    17461 cggtaaatgg tattgagctt actgcggcca tttgctacga gatcattctt ggcgagcaag
    17521 tgcgcgataa cttccgcccg gataccgact atctgctgac tatctccaac gatgcgtggt
    17581 ttggtaagtc tattggtcca tggcagcact tccagatggc gcgaatgcgt gcgctggagc
    17641 tggcgcgccc actgttgcgc agcaccaaca acggcattac ggcggtgatt ggcccgcagg
    17701 gtgagattca ggcgatgatc ccgcagttca cccgcgaggt gttgaccact aatgtgacac
    17761 cgaccaccgg actcacaccg tatgcacgta ccggcaactg gccgctgtgg gtgctgacgg
    17821 cgctgtttgg ttttgccgct gtgctgatga gtctgcgagc acgtaaacgt tgatgtgcct
    17881 gatgtatcct gcatgagcaa aacgtacttt ctcattgagc tttggggcat ggttatcatg
    17941 cccctttttc ttacggttca gggatacaag ccacattgcc atattgggga tgacgaataa
    18001 ttgcctgcgg atcaagcgag cgcgtcctgt ccatttggat caacccgttc ttcgccatga
    18061 caaatatttt tctaccatcc gctgaaaaac gaaggtgatt aacgtagctg gcaggataaa
    18121 cgccaataat ctgcccggca ttaatattga gcaggatgac ataaccttgc aggccatatg
    18181 acaccaacgc taaatattga ccgtccgggc taataccctc aacggaataa acgctcctgc
    18241 tacaccattg cgtcataatg ctggcttgtt gatcccctac gcttatacgt gaaatcccct
    18301 gactatcggc aaaaataacc tcgccgtttt caggtcgaaa acgcatcgcc gatatcgttc
    18361 cacgcgcatc acctgcggga aagtcttctt tctgatgcgt gatggtattg atccgcacga
    18421 tgttacctct gcctgctccc ggaaccagta atgttctttc atccggggaa agtgccataa
    18481 cccaggcgcc tggtttaccg ctcgtccccg tcccgtcgta tttctccagc gggatatccg
    18541 ccacatgctg ataagtcacc agatcaatcg ctgacacact attactggtg cgattcatca
    18601 cccaggcttt tgtgccatca cgcgagagga taaaactgtc aggatgtgca ccggtggtaa
    18661 ttgactgaat aatctcccct gtcgccacgt taatgaccga taacgcattc gcttcaaatg
    18721 acgtcgcata aatcaacttt tcatccgggc ttaatgccag cgatctgacg ccctgatctt
    18781 tacagccacc acaatgtttg actgactttt ctttcgctat gggcgctgta aaggtatgca
    18841 ggattttctt ttgtccgagg tcgaaaacaa caatgctgtt ggtgttttca attgcggcat
    18901 acaacgtatt accatcccga ctgatcgcca tatcatggat atcggcaatc tcagcgagta
    18961 aatcaccact ggcctgttca cgacttaccg gagcggataa tgtttccctg agggcgttgc
    19021 gttcctccgc taactgttgt tttttttgtc gtcgctcttg ttcaatctcc tgcggataaa
    19081 acgcttcacg caggctttgc ctgacctgta aaactgactc atccatctgc tgctgcgcaa
    19141 gccagatagc cccggtcaga agaagactgc ccgcaacaat aatcccgcct tccgtaacga
    19201 gtctgttttg cccagataaa cgcgcgctca gccaacccaa cgcgccaccc gcaatcaggc
    19261 cgccaatgtg gcaagcgtta tctattcctg tttgccgggc accgtacaac agtgtcagcg
    19321 ccaccatacc caacagagga aatactcgcc gctggttttt atggtatgtg cccgtacctg
    19381 aagcaaactg tgttgctatc gctgcaccag ctattcccag aatcgccccg gatgccccaa
    19441 cgctgatgac tatctgctca ctttcgcgta acgcataaca ggcgctgatg agcccaccaa
    19501 agacgccgga gaataaccag gtaatcagga gtcgccattt ccccagaatc tgttccgcca
    19561 cgcctcccac gaccaggaat gcaaacatat tcatcagcag atgagaaaca ctactgtgca
    19621 gaaagatact gctcaataag cgccagggtt cacccgacaa agtaagaggc gcaacgtttg
    19681 caccgagatg gataagtgcg gattgatcga cctcttcata actcatccat tgtgcgagat
    19741 aacaaaatat cccaatacag agtgcaaaaa ggataagggt tattgcgggt aattgaacat
    19801 ttaacggctt aaccgaagat gcagacataa ggtgtttctt ccttgaaaag acaggtaaaa
    19861 agcatttttc ccgtgtgcag ttatcatccc ggcaaacgtc ccatgccgaa aagataattc
    19921 tgtccgaaat atgacttttt cagcctaaaa ttacaatcgt aattaattcg cccctaagta
    19981 ttttttggca tgtaagttgc tttatttact gtgtaagcga tgaaatcagc cgcgacgcaa
    20041 ccaggttgca ccaccacaaa tcagcggtag caccaaaaag gtgcggttta agaattttgc
    20101 ttcggaatgg tgcggcgcat ctcgcaaaaa caaacaataa aacagcaata tctttacatt
    20161 tagccaaact aaatgttacc taacaggcac aacactgcac aataaagttg cagacgataa
    20221 caacacaaac actcacaacg ggtatccatg cgttcttaac gcagaagata aaggagttgg
    20281 atatgcaatt acgtaaacct gccacagcaa tcctcgccct ggcgctttcc gcaggactgg
    20341 cacaggcaga tgacgccgcc ccggtagcgg gcagtacgct ggacaaaatc gccaaaaacg
    20401 gcgtgattgt cgtcggtcac cgtgaatctt cagtaccttt ctcttattac gacaatcagc
    20461 aaaaagtggt gggttactcg caggattact ccaacgccat tgttgaagca gtgaaaaaga
    20521 aactcaacaa accggacttg caggtaaaac tgattccgat tacctcacaa aaccgtattc
    20581 cactgctgca aaacggcact ttcgattttg aatgtggttc taccaccaac aacgtcgaac
    20641 gccaaaaaca ggcggctttc tctgatacta ttttcgtggt cggtacgcgc ctgctgacca
    20701 aaaagggcgg cgatatcaaa gattttgccg acctgaaagg caaagccgta gtcgtcactt
    20761 ccggcactac ttctgaagtt ttgctcaaca aactgaatga agagcaaaaa atgaatatgc
    20821 gcatcattag cgccaaagat cacggtgact ctttccgtac cctggaaagc ggtcgtgccg
    20881 ttgcctttat gatggatgat gccctgctgg ccggtgaacg tgcgaaagcg aagaaaccag
    20941 acaactggga tatcgtcggc aagccgcagt ctcaggaagc ctacggttgc atgttgcgta
    21001 aagatgatcc gcagttcaaa aagctgatgg atgacactat cgctcaggtg cagacttccg
    21061 gtgaagcgga aaaatggttt gataaatggt tcaaaaatcc aattccgccg aaaaacctga
    21121 acatgaattt cgaactgtca gacgaaatga aagcactgtt caaagaaccg aatgacaaag
    21181 cactgaacta attacaagaa ccaggggcgg aaattccagc cctctcgatt gttacgtagc
    21241 acggacagac tatacgcctg atggtcgttc cccatcgggc ctgaaaaccg caatacgctg
    21301 ggtaacaatc ttcgagggta gcagttaacg ctgctaccct cttttttctg gagtagattt
    21361 atgtctatag actggaactg gggtattttt ttacaacaag ccccgttcgg caacaccacc
    21421 tatcttggtt ggatctggag tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg
    21481 attatcgctt tcctcgtcgg ttcatttttc ggcattttac gtaccgttcc aaaccgtttt
    21541 ctctccggtc tgggtacgtt gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa
    21601 ttctttacct ggtatctggt gatcccggag ttgctgccag agaaaatcgg tatgtggttt
    21661 aaggccgagc tggatcccaa tattcagttt ttcctttcat ccatgctctg cctggggctg
    21721 tttaccgccg cccgtgtttg cgaacaggta cgcgccgcca ttcagtcgtt gccgcgtggg
    21781 caaaaaaatg ccgcgctggc gatgggcctg acgctaccgc aggcttatcg atacgtactg
    21841 ctgcctaatg cttatcgcgt tatcgtcccg ccgatgactt ccgagatgat gaacctggtg
    21901 aaaaactccg ccatcgcctc gactattggt ctggtggata tggcggcgca ggcgggtaaa
    21961 ttgcttgatt actcagccca cgcctgggag tcgtttaccg ctattacgct ggcgtatgtg
    22021 ttgattaacg cggttatcat gctggtaatg acgctggttg aacgtaaagt tcgcctgcct
    22081 ggcaacatgg ggggcaaata atgtacgagt ttgactggag ttccattgtc ccttccctgc
    22141 catatctgct cgacgggctg gtgatcacgc tgaaaatcac cgtcacggca gtcgtgattg
    22201 gtattttgtg gggcaccatg ctggcggtga tgcgcttgtc cagctttgcg cccgtagcgt
    22261 ggtttgccaa agcctacgtt aacgtattcc gctcgattcc tttagttatg gttttgctgt
    22321 ggttttacct gatcgtgcca ggttttctgc aaaacgtgct gggattatca ccaaaaaatg
    22381 atatccgttt aatttcggcg atggtggcgt tttcaatgtt tgaagcggcc tactattcag
    22441 agattatccg tgctggtatt caaagtattt ctcgtggtca gtcgagcgcc gcgctggcgt
    22501 tgggaatgac tcactggcag tcgatgaaac tgattattct gccgcaggcg ttccgcgcga
    22561 tggtgccgct tctgctcact cagggcatcg tactgttcca ggatacctca ctggtgtatg
    22621 tgttatctct ggccgatttc ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc
    22681 aggttgagat gatcctattt gccggatttg tttatttcgt tattagtctt agcgcgtcgt
    22741 tgttggtcag ctacttgaaa agaaggacag cataatgatt accctgaaaa atgtttcaaa
    22801 atggtatggt cactttcagg tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt
    22861 ggtggtggtt tgcggcccgt ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg
    22921 cctcgaacca gtgcagcaag gtgaaatcac cgtcgatggt atcgtggtta atgacaagaa
    22981 aaccgatctg gcaaagctgc gttcccgcgt cgggatggtg ttccagcatt ttgagttgtt
    23041 cccccatttg tcgattattg aaaacctgac tctggcgcag gtgaaagtgc ttaaacgcga
    23101 taaagcgccc gctcgtgaaa aagcattgaa attgctggag cgcgtgggac tttccgcaca
    23161 tgccaataaa tttccggcgc agctttccgg cggtcagcag cagcgtgtgg caatcgctcg
    23221 cgcgttgtgt atggatccta ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc
    23281 ggagatgatc aacgaagtgc tggacgtgat ggtggaactg gcgaacgaag gaatgaccat
    23341 gatggtggtg acccacgaaa tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat
    23401 ggacgagggt aaaattgtcg aagactcgcc gaaagacgct ttcttcgatg atccgaaatc
    23461 ggaccgcgcg aaagacttcc tcgcgaaaat cctgcattaa tcactctggc gcgcataccc
    23521 aagtgcgcgc catctcacgc tttatcgttt cgcccctcgt atctgttaca tcctggcgtt
    23581 acccttgtcg caaagaagca cacaacaagg agcaacaatg gcactgccaa ttctgttaga
    23641 ttgcgaccca ggtcatgacg acgctatcgc aatagttctc gccctcgcct caccagagct
    23701 tgatgtcaaa gcaattacgt cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa
    23761 tgttctgcgt atgctgacct tgcttaatcg caccgatatt ccggtagcag gcggcgcggt
    23821 aaaaccgtta atgcgtgagt tgattatcgc ggacaatgtg cacggcgaaa gcggtctgga
    23881 tggcccggca ttaccggaac cgacgttcgc accgcaaaac tgtacggcgg tagagctgat
    23941 ggcgaaaacg ctgcgcgaaa gtgcggaacc tgtcacgatt gtgtctaccg gaccgcaaac
    24001 taacgttgcc ttgctgctca atagccatcc ggaactgcat agcaaaattg cccgtatcgt
    24061 gattatgggc ggcgcaatgg ggcttggtaa ctggacgcct gcggctgaat ttaatattta
    24121 cgttgacccg gaagcggcag aaattgtctt ccagtcaggg atcccagtgg tgatggccgg
    24181 tctggatgtt actcacaaag cacaaatcca cgttgaagac accgagcgtt tccgcgcgat
    24241 tggcaaccct gtttcaacca ttgttgccga actgctggat ttcttcctcg aatatcataa
    24301 agacgaaaaa tggggctttg tcggtgcacc gctgcatgac ccatgcacca tcgcctggct
    24361 gttgaaaccc gagttgttta ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa
    24421 atatagccag ggtatgacgg ttgttgatta ttattatctg actggcaata aaccgaatgc
    24481 cactgtaatg gtcgatgttg atcgtcaggg ctttgttgat ttactggctg atcgtctgaa
    24541 attttacgct taacacacgt cattgattaa gatcaccacg cgttacctgg gtggtcttaa
    24601 tctgtttatc tctttttctt tcaagatata aatgcaatgg ataatgcaga actcgccatt
    24661 ggtatcgatc tcggcactac caatagttta attgccgtct ggaaagacgg tgccgcgcaa
    24721 ttaattccaa ataagttcgg tgaatattta acaccatcca taattagcat ggatgaaaat
    24781 aatcatattt tagtcggaaa accggctgta tcacggcgta cttcgcatcc ggataaaacg
    24841 gcagcgttat ttaaacgtgc aatgggcagt aataccaact ggcggttagg cagcgacaca
    24901 tttaacgcgc cagaactgtc ctctttggta ttacgctcat taaaagaaga tgccgaagaa
    24961 tttctgcaac gtccgattaa agatgtggtg atctccgttc cggcttattt cagcgatgaa
    25021 caacgcaagc atacccgttt agcagcggag ttagccgggt taaatgcggt acgcttaatt
    25081 aatgaaccca cagcagctgc gatggcgtat ggcctgcata cccaac
//
LOCUS       DAZFDP010000061        24729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFDP010000061 DAZFDP010000000
VERSION     DAZFDP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1845)
                     /gene="traD"
                     /locus_tag="ACHJ1G_002526"
     CDS             complement(<1..1845)
                     /gene="traD"
                     /locus_tag="ACHJ1G_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO4764366.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQ"
     gene            complement(1896..2633)
                     /locus_tag="ACHJ1G_002527"
     CDS             complement(1896..2633)
                     /locus_tag="ACHJ1G_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764367.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2836..3567)
                     /gene="traT"
                     /locus_tag="ACHJ1G_002528"
     CDS             complement(2836..3567)
                     /gene="traT"
                     /locus_tag="ACHJ1G_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO4764368.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3616..4101)
                     /locus_tag="ACHJ1G_002529"
     CDS             complement(3616..4101)
                     /locus_tag="ACHJ1G_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO4764369.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(4117..6939)
                     /gene="traG"
                     /locus_tag="ACHJ1G_002530"
     CDS             complement(4117..6939)
                     /gene="traG"
                     /locus_tag="ACHJ1G_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO4764370.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(6936..8309)
                     /gene="traH"
                     /locus_tag="ACHJ1G_002531"
     CDS             complement(6936..8309)
                     /gene="traH"
                     /locus_tag="ACHJ1G_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO4764371.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8296..8688)
                     /gene="trbF"
                     /locus_tag="ACHJ1G_002532"
     CDS             complement(8296..8688)
                     /gene="trbF"
                     /locus_tag="ACHJ1G_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO4764372.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(8669..9016)
                     /gene="trbJ"
                     /locus_tag="ACHJ1G_002533"
     CDS             complement(8669..9016)
                     /gene="trbJ"
                     /locus_tag="ACHJ1G_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO4764373.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(8946..9491)
                     /gene="trbB"
                     /locus_tag="ACHJ1G_002534"
     CDS             complement(8946..9491)
                     /gene="trbB"
                     /locus_tag="ACHJ1G_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO4764374.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(9478..9762)
                     /gene="traQ"
                     /locus_tag="ACHJ1G_002535"
     CDS             complement(9478..9762)
                     /gene="traQ"
                     /locus_tag="ACHJ1G_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO4764375.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(9881..10225)
                     /gene="trbA"
                     /locus_tag="ACHJ1G_002536"
     CDS             complement(9881..10225)
                     /gene="trbA"
                     /locus_tag="ACHJ1G_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO4764376.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(10239..10982)
                     /gene="traF"
                     /locus_tag="ACHJ1G_002537"
     CDS             complement(10239..10982)
                     /gene="traF"
                     /locus_tag="ACHJ1G_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO4764377.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10975..11232)
                     /locus_tag="ACHJ1G_002538"
     CDS             complement(10975..11232)
                     /locus_tag="ACHJ1G_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO4764378.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11259..13109)
                     /gene="traN"
                     /locus_tag="ACHJ1G_002539"
     CDS             complement(11259..13109)
                     /gene="traN"
                     /locus_tag="ACHJ1G_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO4764379.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13106..13744)
                     /gene="trbC"
                     /locus_tag="ACHJ1G_002540"
     CDS             complement(13106..13744)
                     /gene="trbC"
                     /locus_tag="ACHJ1G_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO4764380.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13753..14058)
                     /locus_tag="ACHJ1G_002541"
     CDS             complement(13753..14058)
                     /locus_tag="ACHJ1G_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764381.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKNADISEQVTLVRLYLKVTFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            complement(14088..15080)
                     /gene="traU"
                     /locus_tag="ACHJ1G_002542"
     CDS             complement(14088..15080)
                     /gene="traU"
                     /locus_tag="ACHJ1G_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO4764382.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15077..15709)
                     /gene="traW"
                     /locus_tag="ACHJ1G_002543"
     CDS             complement(15077..15709)
                     /gene="traW"
                     /locus_tag="ACHJ1G_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO4764383.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(15706..16092)
                     /gene="trbI"
                     /locus_tag="ACHJ1G_002544"
     CDS             complement(15706..16092)
                     /gene="trbI"
                     /locus_tag="ACHJ1G_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO4764384.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16089..18719)
                     /gene="traC"
                     /locus_tag="ACHJ1G_002545"
     CDS             complement(16089..18719)
                     /gene="traC"
                     /locus_tag="ACHJ1G_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO4764385.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(18845..19192)
                     /locus_tag="ACHJ1G_002546"
     CDS             complement(18845..19192)
                     /locus_tag="ACHJ1G_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764386.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(19220..19438)
                     /locus_tag="ACHJ1G_002547"
     CDS             complement(19220..19438)
                     /locus_tag="ACHJ1G_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764387.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(19518..19994)
                     /locus_tag="ACHJ1G_002548"
     CDS             complement(19518..19994)
                     /locus_tag="ACHJ1G_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764388.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(19987..20208)
                     /gene="traR"
                     /locus_tag="ACHJ1G_002549"
     CDS             complement(19987..20208)
                     /gene="traR"
                     /locus_tag="ACHJ1G_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO4764389.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(20343..20858)
                     /gene="traV"
                     /locus_tag="ACHJ1G_002550"
     CDS             complement(20343..20858)
                     /gene="traV"
                     /locus_tag="ACHJ1G_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO4764390.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(20855..21175)
                     /locus_tag="ACHJ1G_002551"
     CDS             complement(20855..21175)
                     /locus_tag="ACHJ1G_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO4764391.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(21162..21728)
                     /gene="traP"
                     /locus_tag="ACHJ1G_002552"
     CDS             complement(21162..21728)
                     /gene="traP"
                     /locus_tag="ACHJ1G_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO4764392.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPVLPGKMKCIRVVTADEYLKDVYTEDAS
                     LPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(21718..23169)
                     /gene="traB"
                     /locus_tag="ACHJ1G_002553"
     CDS             complement(21718..23169)
                     /gene="traB"
                     /locus_tag="ACHJ1G_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO4764393.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(23169..23897)
                     /gene="traK"
                     /locus_tag="ACHJ1G_002554"
     CDS             complement(23169..23897)
                     /gene="traK"
                     /locus_tag="ACHJ1G_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO4764394.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(23884..24450)
                     /gene="traE"
                     /locus_tag="ACHJ1G_002555"
     CDS             complement(23884..24450)
                     /gene="traE"
                     /locus_tag="ACHJ1G_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO4764395.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(24472..>24729)
                     /gene="traL"
                     /locus_tag="ACHJ1G_002556"
     CDS             complement(24472..>24729)
                     /gene="traL"
                     /locus_tag="ACHJ1G_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO4764396.1"
                     /translation="NQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGAAVLVYFGIK
                     KLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
BASE COUNT         5955 a         6833 c         5743 g         6198 t
ORIGIN      
        1 ctgagtcaca tcttctccgg aggcaacctc cggttcaaag aggctggcca tctgacgacc
       61 ttctgcttcc cttgcggcaa gtacggcact cagacggttc tccatttccg ggttgatgtc
      121 acgcggaata aactccggag cgaccttcgg tcgtgcctga tatttcagag agagtttgac
      181 tgccggatac ggtccgggca gagtgacata gcaggtcaga tccggcagag actgaatgtc
      241 ggaataactg accagcgtct gacgctccat atccttaccg gtcgataccc cgtcacgtac
      301 cgggtcagca ccgtaggaat actgctcgct ggctttcagg tgctctttct caccaatctc
      361 accggcagcg aactctgcaa tcttatggct cggggaacgg aaaaaagcac gggtgttcat
      421 gacgtcaaac agcgtggccg ccgctttctc accgtagata tcttccagct gggcatagga
      481 ctggatacca aacacataac agccaccgaa cttacgggct tccggcagga tctccaccag
      541 atccggcagt ttgtgtaacg tgggtaactc atcacagaaa aaccacacac gacggttacg
      601 gttttctccc attgccagca gaccacgaat ggcaatggac agccacatgg agatcacagg
      661 cttcagggag gcatgggtgt cggcgttcga cgaaataaac agccagccgt ttttctgatc
      721 ttcccggaca ccacgcatcc agtcacggat ggtgaaggga tcaccgttat gctcaatccc
      781 ctgcaggtaa cggatggctt tcacgtagtt ggtcagcaca gcacggatgg aaatcgccgt
      841 tttctcaatt ttctcttcca ccaggttggc cgccggtgaa ttacgcagga aggtacgcag
      901 tttttcaatt ttgatggaaa gcagtgtgtc caccagtttg ctgtagctgc ggttggggtc
      961 attacgcatc aggtacgccg cttccgcaaa aatggtacgt cctgaaccct gccagaacgg
     1021 gtcctctttt gtacccatag ggatcagcgt gttcgcggta ttatcaaaat ccggctgtgt
     1081 caggcactcc ttccacaaat cccatgcggc acagcgggca tccagcggat tcaggatttt
     1141 atcgatggag ggatcatagt aacttttaac aaattcccct gaacggtcat aaatcaccac
     1201 catatcaccg cgctgacggg catagttggc cagacgacgg ataacctccg acttaccggc
     1261 cccgaccgtg ccgtgcagac agaagttctg gatttcggaa tcccggataa tcggcaggtc
     1321 gccaatccgg atatcggagt ccttgccgtc ttttttcagc atccgggcaa cgtctttcgg
     1381 attgtctgtc agctgacgac cacctgtgac ttcattttcg ctctgttgtt taccctgacg
     1441 ccccagaatc caggagacaa caaagaaggt gatcaggcaa ataaccaggg ccacgacaga
     1501 cgccagaacg aatgcagacc atagctgctc tccgcaccag atcatatatt tatcatgcag
     1561 tacctgggca gcgttcatcc ggaaggtttt gccgtaatac tggatctcat agaccggctg
     1621 ggattttatt aaatcccgca tgccttccag tgacgtacac caccagtaaa tacagccgtt
     1681 gataaacgtc tgccagctta tttttaccca taagaccaga ccaatgagga tccagaaaaa
     1741 aataaacagg cagtaaagca tgatattggc gatctggctg aacatgcgga tacgcatgga
     1801 cgcaatctga ccgccctggg tcatatcctt tgcgttaaaa ctcatgatga aactccggga
     1861 aaatctggaa cagggcagaa aaggatgatg tggggttaat tcacagaaac agaccaggtg
     1921 taccctgtat gccggtggtg atgcccgcct gcagtcctgc cggaagagac cttctctgta
     1981 acaatgtcag tcaggcgcaa tacttcagac cagtcactcc catgcggaaa ctggatctga
     2041 ataaaaaggc gtacccgttc acggtgatcc agaactgtgt ccggttcata aaaacggcca
     2101 ctgtcaggag aaacacagga cgttttattg atgagatcat acttatcggc aggagactgc
     2161 gatgaaatcg cccaggtgat attctccgcc tggtggccat caatacgact gctccccggc
     2221 gtccatgcaa tttcctgcgc actcgccacg atgatctccc tggaaacttc agccagcttt
     2281 ctgggtgtac agaataccgg tgccagcaca cacagggaca acaaattaca gctgaccgcc
     2341 tccggtttaa gcaaattgct cccggatttt tcaggaaaag atatttttgt cagtatttca
     2401 gttaattcct tatgcctgag agaattaata aagtacatga taagcatcat gagaaacaat
     2461 ataacaataa gccctccgat aaccactaaa aggtcagtat caatattatt gctgtacaga
     2521 tagtctttag tggtcactgt attatcctgt atcaccgggg ggtattcagt tacaggcttc
     2581 tcagtaaatt gattattctc accaacatat tcagcgatat tgtctgatgt cataatgact
     2641 ttctccggaa gatcccggct tcgccggcaa cacttcacgg tcgcacaaag aatttgcaca
     2701 accgctcagt gttttcatat ttatttcgga aacgccggga ggtgacaaaa tttattttgt
     2761 tacatcccgg ggttggacaa cgagcaacgc gaggcgtcag caaggaggcc ggtcagatca
     2821 gcctcctgac gaggttcaga gaatatttgc gattgatttg gccagttggt cttcgagaac
     2881 aggcttcgcc tcttcaaatt tcaggttaac cttgttcgca tttgaaacca cacgggtctg
     2941 gtatttatgc tggttacctg tttcagtact ggtctgaatt ttcgcacctg atgtgccctg
     3001 acgcagggcg gcaacattat ccgttgtcac cgttgcctta gtacgctctg caatctgtac
     3061 atccgtgatc atggtatagt tcacatcttc caccatcgca tctgcagcca tacccaccag
     3121 accagcagca aggcctacac cgagtgtggc accggcagaa tttgagttat agccggtaat
     3181 accggcacct aacgctgcac caactgctgc gccttcataa ccacggttca gccatccctg
     3241 agactcccgc agatccatct tatcggcctt cagcacattc gcctgaatcc agtagtaggc
     3301 tttatccgga gaagtcacca cctgatatcc ttttgctttc acagcatcag caattttgcc
     3361 ctgcagccca ctcatgtctt tatcagacgt gtttttgatc tgcagaaata ccgtgcgttc
     3421 gctggcgggt tcaagccaga tggtctcact catctgagtc ttcacctcaa ggttacgctt
     3481 cttgattgct gtgctcatcg caccacaccc tgaaagggcc agagtggaac tgaccagtgc
     3541 aaccatcatc aattttttca ttttcatcaa tgttccttca tattgaataa aacgtcataa
     3601 tatacaaatt aagattcact catcatatcg ttttgaacta taaccatatg aattaccacc
     3661 gatatccaca ccaatacctg acataggtaa ccctgttgca ggattagtac gatgaaaatc
     3721 catattatca aaatccagat gcaaatcttc attagtttgt tcatttacct gagattgcat
     3781 ccattctctt acgcttacac aaccactaac tcgccttaat tcgtatagtg taccaatgat
     3841 aaaaaccatc aggcttaata atggagagag taaggaagat actggcatcg ttatatacca
     3901 aacgccttta ctaaacttaa actcatccga cacattccgt ttttttcgta tttgagagaa
     3961 caaccatata aacagtccct gccatagtac tatgaagaac agcataaaaa ttgtttcggc
     4021 attcccccaa ctagccagat aacaggcgat aaactgtatc accgttacca gagtaatatg
     4081 ggttaaacat ctcattatca tctcccaaaa acagcattat tgcttatact tatcctgata
     4141 ttcctttgcc cttttcatca gttcctgtgg actgtcagcc ccgggcattc tttcctgagc
     4201 cgacttttct tcattatatt tattattctg agataatgcc tctgttttat ggtgattctg
     4261 cagttcagaa tactgtcctc ttacaatatt ttcctcgcca cgtacgctgt tctgagtatc
     4321 acctatattc cctttatatt ctgtgaccat attatcaacc tgatgtttta catcattacg
     4381 gatattactg tcctgcgttc tttgatcaat aatggcctga tgcccctgat ggtcagcaat
     4441 aatatcctga ctgccaccac ccgaaggtac gctctccatt cctttaccaa tatcaccacg
     4501 ggattcccgc caggcattat caacaccagg ctgtacctgt tcctgcacaa aagaccaggc
     4561 catcgcccga cgacgttctg caatctccgg tgaactggtg tttgtcagaa tagcttctgc
     4621 atcctgtggc gcgtgtttca ttacatactg tgcaaactgt tgcgacaggt cttcactcat
     4681 ctgcccactc atgctttcag tacgggatgc catttcagca tattcatggc tgcgggtcat
     4741 attcgtcgtg tactggtcgt aactctgctt cgctgaattc agggctgcag acaactgatc
     4801 cacacgggaa tcagcattat tgtccgtatg gctgccggac tcactgacct tgcgactggt
     4861 aaaataatcg ctggcctctt taaagtcttg tgtagctctg gcatcgatat catgacgagc
     4921 attatggctc gactgagtat tactacttgc cgtatgcgca tcattatctt cccaatcaat
     4981 accagcccga ccaccacctc taacgcctaa tcctccacca acaccaagaa ttttaggttt
     5041 aaccccccat tcagcatgag catctccata catcccggca gaagctctcg tacttctgga
     5101 tgccagttcc tgagtcgcct gctcattact gatattatgc gccttcgcat agctttccac
     5161 tgcactgcgc atacggctgg ccatcatgga gtcctgtgcg ctcatcgtgc tgtcagcacc
     5221 acctgtgaca gaatcactgc tcccccggtt agaaccaaac tggctgagcg agttccaggc
     5281 actggcaata ctgctgctga acccatgcag ggcgctttct gctctgttcg acgcctcccg
     5341 ggccatttcc tgttgtgccg ctgcaatctg acgcgttgca ttgataccga ccggtaaccg
     5401 ggacatcgcc ccgctcgcat ccatcaccat attaccgtca cgggtctgtg tggcagttgc
     5461 gccactgccg gtctggtaca tcatctgacc aaacgacgtg gtgctgttgg tgctccagct
     5521 gaagccgttc acgttttccg tctgcatgtt gccgtaggag taattaccgt caaccacact
     5581 gccagctgca ctggcagtcg ggctgatagc agaggaagcg aaatggctgt acacgctgga
     5641 aaaacctgcg ccaaggcctt ttaccatcat ccatgacaac gggggtatca tgacagatat
     5701 atatccggca gtggacgcca ggtcggagta tttcagctgt atctgagaga gttcagagag
     5761 cacgaccggt gcaccattct gctttgcata gaatgtcatg gcactgttca gaatggcata
     5821 cagcaacggc caggtctgga accacatcag agcaaacaca tagcctctca gaacagacag
     5881 cgtcagtctg ttgaagacag ccgccagtac caggagcggg aagataccaa tggtaatgcc
     5941 caccagaatg gtctgcacca tcggcaggtt tcgcatcagc acatgaccaa tggagacatg
     6001 tgccagacgc tgtttctcca tcgacgaagt cgtggccaga ctgaccaggc tggcggtatc
     6061 accgttacgg gctgcattac tggtgatacc ctctttcagg gcattcatgg tgacgttctg
     6121 gcgcataata tgactggcag actggctgga gccgtagaaa taactgtaac tgtcactcac
     6181 cagttgcctg aataacaggt ccgggtccgg tctgccgcca aatatctgct gcacataata
     6241 atgccaggtc ttgcctcccg tctttgtatc gagattcagc ctgtctttca gcgtgaccga
     6301 cgcatcttta caggttatga aattattatt gctgtcataa acgccccgta gcgggctggg
     6361 ccgggaaaaa atcagcgtgt aggggtcagc ggaggccatc aggtcttcca gcgtgtattt
     6421 gtggttcagg taaatatccc ccagcacgca gttctggacg tagtcctgaa acagattgat
     6481 gatttccgga ttccgggaca gaaaatcggt gcttttcaca atcaggttcg ccccgaacag
     6541 catgcctgtc ttgctgtagg tggcgctgtc aggctgggtg aatatcatct cgtaactggc
     6601 caccattgca tgcccgatac gggtcgtcag tgaaagtggc atcgccagtc cgacaggcac
     6661 attatccacc cggtgaacct ggaccaggtc actgttatca ataatctgca ccgatgtacg
     6721 gacattaacc agcaggctga taagcacaaa cacggccacc cagcccagca aatccatcac
     6781 gttgtgccgc tgtacccaca tcacggccac cgcgagaaca gacaatgtga gcgcaatttt
     6841 ttcgatggaa tcccacgtcc gggtccccat aaaggcggca atggcgttca ggttattgcg
     6901 taaccactca ccaccggcaa tcacatagac ttcattcaca gcgtgctccc tccgaagtga
     6961 tagttgttct ggtaacggct gagcatgcgg gcggaaagct gctggcgcat atagctcatc
     7021 tgacggtcaa caaccagcaa tgcatcctgc tggaccttta cctgcccctg gaaaaccgca
     7081 atctgttgct gtgcctgatt cagattctcc agcacactgt ccagcacggt ctgtggatag
     7141 ttcccggtgg caatcatggc ccgggcctgc tgaagcagtt cctgaatgta ctggagcatg
     7201 atgtcgtagc cgatatagtc cgtcagctgg taaaccacgc tgctggatac accgagcatc
     7261 tgcgggtcaa taagatattt aaagacgggg acggtggtgc tggaaatgaa gcctttttcc
     7321 ctgtcatcca gtggagtatc actcaccgct ttattctgaa tactggtgag cagttttgta
     7381 atctgtgatt tcagggcttt atcccgggcg atggtcacat ttgcatccgc caccaccttc
     7441 agacacttgt ccgaatcgtt gcagtgataa acttttgccg ttcccccctc cataatcgcc
     7501 cggaggatat cgcggtcagt ggtacgcgcc ggcagggggg taatctcgct gtcttcccca
     7561 aaaatcagcg taccggtgag cgtcataata aattctttca gttctttgtt accatcaagc
     7621 atcctgtttt tggacagcga gtcccagatg atattgatgt tcttcagcac ccgttcctta
     7681 tctttgtcac tggccttgtc acgaacagac gatgactgtc ctccgaccgt gcagccctga
     7741 cgggacgcgg cccagtcagc aaaaatattg ctctcaccgg caatgtcctg acagaccttc
     7801 tgctgtgaca cctgagtctg aggaaataat cctccgacaa tcccctgcgc cacctgacag
     7861 gagctgaggt tcatactgtt aatgtcgctt gccattttct gcaggaagtc ttttgcggtt
     7921 ttgatttccg gcaccgttgc ctgcagggca aggtcaaaaa agtaaccggc agcgttgctc
     7981 ataatctgct taacaaaacg ctgcaactga tcgccattaa taaaactgaa cgagccgaga
     8041 taggcgtcga tgccgccaca tccggcattg atatccggca gcgtcatgga aatcagctgg
     8101 atatttttga cctgggtgcg ggcatacagg gagccaccat aggcataccc ggcggcctgc
     8161 ccctgccaga cgcccggttg tgtggtattg gaagcaaatc ccagcttatt aaagaactga
     8221 ttcatgtcgc tgttcacatc cgctgacgct gcgggtgtga ccgtcagtaa agcggcacaa
     8281 agaaccagaa gaggtttaat gcgaagcatt attgccaccc tcccttaatt tcttctcccg
     8341 gcgcatacgg cgtttcagcc ggaatattct gatccccccg tttattaatt catttaaagg
     8401 gttcctgacg tatacgataa gaaatccacc tgcaaacgcc cataacgtat acggaatgac
     8461 atacatcagg atgttccaca tataacgaaa attatttaat tccgggtcag ctttccacga
     8521 atcaatacag gcatcaaaaa tactatgcat aatcacccat aaaaaagttc cggcagcaac
     8581 gaacacaaaa aacagatcaa aatatatatc agaaaccctg cgttcataat cataatcccg
     8641 tttagttttt ctgtttttta gttccgcatc atttcttttt tctttcatta taagtttcct
     8701 ctgatatttc cgatgattca ttcagaccgt tcatgcggct gtaatatatt tcagacaccg
     8761 aaatccatcc cacaattgcc cccacaaaaa acacaacaga aacagaaaag gtaagacaca
     8821 gcgaaacgac aatttccaca gggctggcgg gataaaaaag cccctgacgg ataaagggga
     8881 tattcaggct gacgattatt cccgtcgcga tgccggatat tccggcaatg agtaacacca
     8941 cctgcttatt tcgtaccttt ttcctctccg tacatctgca gaacggtatc catccgcgcc
     9001 ataaaactgg cggcatccgt tgccccctgc aaaagcggta atgcctcaag tgtgttgacg
     9061 ttgaccagga aggtagtcgg tgtggccacc gggatattcg ggaagaaggt ctgcatcacg
     9121 tccggtggca ccggtaatgc ttccggaaag gcggtatccc cctgaccatc cagggtgtag
     9181 ggaaaaacgg aaaagccgta ctgctgtgcc agctgtttca gtaccgggtc aaactggtga
     9241 cagtaagggc aatgcccctg cataaacagc accaccttcc agtcggccag gttaacctgc
     9301 ctgccgttgc tgaggcggaa ccagcgggga gccggctttt ctgttctggc tgatgtgtct
     9361 gctgccggtt gtgcaggctg cacggccata ccctgcggat tccagagtcg ttcaatttca
     9421 tcccgggtgg aggcctgcac acctgtggcc atcatcaaca aaatgaccgg cagtaattta
     9481 gtgagagaca tgtccgccct cctgctgccc ttccatcatg gcctgttgcc tggcttccag
     9541 tgcctcacgc tcttccaggc tgacacgtgc aatccatgca tccagcacct ggtatgcgcc
     9601 gaacagcaca aggatggccg caatcaactc cgccagaaaa aaagccatcc agggtttgct
     9661 gtaaacaaga cgggcgacga tatgaaacca gacacccagg gaaaataccc acattcccgt
     9721 aatatcaagc cggggtaaag agaatctgta tttacttatc atgttaaact ccggttatta
     9781 tttacatgag agtattcatg tctgtaatat tcgcgtttgt gtgatattaa tcagatcaac
     9841 ctcattattt tgtatggttt ttattaccgt tttatatgac ttagcctgtc ataagccccc
     9901 ttataaaaaa ccacatcact ccggaccatg ctgatgacaa cataatacaa acaaacatcc
     9961 ttctgaaact gtcaaacttc gtgataaccg gtatggtact gactgccatt gaaacaaccg
    10021 gtacggcaca ctgattcata accgcaccac aaagaaacaa gaaattatct ggcagcatac
    10081 atacacttat gacatttact acaaagaaga caaacgagct cagcagataa actcctttat
    10141 tatttggtcc tgtaaagaaa taaccaacaa cccccacgat aaacaggctg gcacctgtaa
    10201 aaaaacacaa atcccctgta tgcatatata acctttggtt aaaaattcgg tttaaaatct
    10261 tcagaaacgt tcaggaactg ttttgccagg tcgtcctgtg aaataaagcc gtatgataac
    10321 gggcgaacac tgccctgttt cgggtcaacc agcatcatgg ccgggaaata tttcacaccg
    10381 aggcgctgcg cctgcccctg gtcagtccgg gaatccggca acagcggatt aatcacgcca
    10441 tccacggaaa cgggaataac actcagacca taggtatccc tgaagccatt aatgacctgc
    10501 gccagttgcc cgtcgatggg gtcctgcccc cggtaaaaga acatgatgcc gtagtgttca
    10561 gccagtttcg caatggcctg tcgctgctgc gcctggtctg ccgccagctg gttccgtacc
    10621 gtgccgttgt aatggctgta ctgcaggtta tagtccagtt cgggatgtgc cagcatggcc
    10681 tttttagcac tcatggtgaa aagcccggcc tgctgagtcc agtaattctg cagccggaaa
    10741 tatttaacaa aattatccac gccgggatac agaatggctt cgtacagcgc ccgctttgtt
    10801 gccgtctgca gcgcggccag tttctgcata atatccggct cctgacgggg ggctgcaggt
    10861 acaggtttat tttctttttc cttcggattt attttctcgt tataccactg ccagcctgca
    10921 tctttccctg acgccggaaa aataaagcag cagagtaaca gtggcagtaa tgctttattc
    10981 atcttcattc ctttcatcat tttttctttc atcatctttc tgcaggccag tcagggcaga
    11041 aaaacaaaaa tacagaataa acaggagcac accggagtaa aggataacga aagcagcggc
    11101 aagcatcccg tctgccgtgg tttcttttac cgtatcccac gaccagataa ttaccgggga
    11161 aatgacaatc gctgtaatcg aaagccggat aagaaaatca acaagacgat tagaagtgaa
    11221 tataactttc atcatttccc ccggttattt tccatacctt actgtcctgc ctgtttcagc
    11281 tggtcagcaa tctgctcttt cactttctgc gtcagaacgc cactgtccgg gattttctgg
    11341 ttattcatca ggtcttcgta gaagttagtg aaatccagtc tgtcgaactg aattttctgc
    11401 agctcatcaa ccgtaatccc ccgacagtca ggatgcttcg cactgccaaa cccgatacgc
    11461 aactgcccgt tacgcccctg ctgctggact atctgcgcca gcttcgaatc aaactggcag
    11521 tagctgcgct ttttctgcag acagacaccc agcactttct tcgaacagaa ctctccgaca
    11581 ctgacggtca gtttgttgtc ctttgccttt cccagcgcct tttcttcgct gttgcacttc
    11641 gccagcccca catcctgacc ccagccacca tccttacagc agttactgaa gccggctgcg
    11701 gccttacggc agaatttcgc ctcacccgtg aaagccctga catccacacc attcagggcg
    11761 gcaacatcct tacctgcggc ggcaagcgca gccagctgtg atactgcttc accaaaatca
    11821 ctgctttttc cgctctgtgc cttatcgcat tcaccgtcaa ggcagaagac atcaccacca
    11881 cagaccatca cttttcctga tgttctggac tcgcaggagt acgtggcgta ctcatgcaga
    11941 caggttccct catcggaata aaacgcgcac tgacgtgagg ccagcgtaca ggccggatta
    12001 tcgacatatt tctgacaggt gccgttatct gcggactgag tgacatattt atcccggtat
    12061 gcccagcatg cctgtgtgat gttccagggt ttaccttcca tcactccggt tttcgtacca
    12121 cccggctctg aacactcagt gcctgtcagc acgccatccg ctttattaaa cggacaactc
    12181 tccacccact ccacccgggg aacccattct ttatcacgga ccttcatcct gagtttcagc
    12241 gtaaaggtgg aagcaccact gacaagcgat tcatagactt tcctgtcacc attcccgtgc
    12301 gggaggcaat taccgttagc agtacagcca ctaccggtca gaacctgccc ctgtgtcacc
    12361 tgcaaacctg tcgccccgga cagggggtaa gtatccgact gcccgtttgc caggccgaca
    12421 ttaaaggttg tattcatgaa ggtataacgg gagttcagga agaaaaaagc ggcgtataca
    12481 gacaatgatg cactttccac tgtaccggtt accggagccg tgacactgaa caccagtttc
    12541 agcccgttca tactgaaccg gaactgtgag tgcggtatcg tcacttcacg atattcatcc
    12601 gtgtatttcc agtccccggt gatactggca gtccgtgtgc agtactcctc caccatcgtg
    12661 tcccgctcac aggtatagtt tgtgaattca ctgcggttta tctcctgcgc actgcactgc
    12721 tgtccggtat ttcccacaat gctgtcagcc cggttcacca catccctgcc ggtctgaatg
    12781 aacggtgcat ccggtgacag aatgtctttc ggcttgttca tgaaggactc tgtgatggtt
    12841 tttccggttt caccggtcgc ccattccgtg gtgccgtcat ttttcaggcc gccatccccg
    12901 ccggcagtca cgccaccgta atattttgtc tcgtccggat tcgcgttata gctggggata
    12961 ctctcctgtg gcttaaagcc ctgaatactg cctgtaccct gcccctgaat ctgacgggca
    13021 aaatcagaac cggcccggta atcactgttg ctgtcagcct gtgccatgcc ggcaaccaga
    13081 gccagtatca gaggtaaaat acgtttcatt tcccggaatc tcctttccct gccagtaaat
    13141 catgagccac ctgccggcag tcacccgtgg cggccacttt ctccagcgcc tggccgaccc
    13201 gcaggtttcc ccggatgatg tcgtatccct gactgcagaa caccaccagt gccggtacac
    13261 tgcgaatgcc gtactgtgaa aacagcgtcg ggtcgatctg aacaccatca gtcgcgccgt
    13321 ctttcaccag ggacagcacg gcttcagcgg tggtcttcag gtcattgttc accatgcccc
    13381 gcagtgtggc gggtataccg tagtgccggg tttcgcccag catccgtttc agcccctctt
    13441 cgggaatgga aaacgacaca aaatacaggg caccctgccg gggtttcccg tcctgcgaag
    13501 cctgctgttt acgaaccagc tcatccagaa aatggttatc tgaacgctga agggggtttt
    13561 ccagcacctg tttctccgcc caggctttca gctgatggtc aggtttctct cgcagttgtc
    13621 tgcttaaatt ttcctgctgc ttcaggaact ggcggttttc agttgtgttc acattttctg
    13681 aagcatgaac agcaagatgc atccctgctg tcagagcaaa aagtgcaaat attttccggt
    13741 tcatgatatt tctcactttc tgctatttac aggcatgaac tcatcagaaa aaagataatt
    13801 gagaaaaaca gccacaaacg cacatgtaaa tgtcaccttc agatataatc tgacaagtgt
    13861 cacttgttct gatatatctg cgttcttcca ttcatgaata atttcagcga gaaaactgtc
    13921 aggtgcaaaa taaaataata taacgggcac tattacaaga ccaaaaggaa ataattttaa
    13981 tgttctgctt atcagcctga gcagtacagc acttattttc ctgaggtgct gaatatattt
    14041 actgtccatt gttgccattt tcatttcctc ttattcatta aaatgattta caggaataca
    14101 cagttacgtt tacgccacat caggtagccg aagtttttct ttgtgttggg cggatttttt
    14161 cccgtctccc agcgcatcac actgcgcccg aacggatggc actgtccgct gtccggatac
    14221 atattcacca tctgataacg ccagcgttct ttaggcagga ttggggacgg atattcatta
    14281 cagacggcgt tatttttccc gatggtttcc ataatcatgc cctgacggtg tagtttgaac
    14341 gccatgcgtt cactgaccag cagggaggac tgcaacggac tggactcatt actcacccag
    14401 ccattgaacg ggtacatact gccctgcgaa ccggcacacc agaacagaac atcgagaggc
    14461 atattaaagg cgctggcaat ggcatctgct gcacaggccc cctgtgctat cggattggca
    14521 aagatgacag cttccggatt gagaatggtg gtcaggctgc tgtccgtcca ggtggggtca
    14581 atttcagaaa gataagcgat atccaggtca ccaccttcca gacagcccag cgatgtgatg
    14641 atgttaagcc agtacgtcag cgggtatttg taccagtgaa cgtgatagaa cgccccgttc
    14701 acctgcttct catccttttt cgccgttccc tgtgccgttt taccaaaagc cggcaggctg
    14761 aagcccaggt tcaccatgca tcccggtgac cgggtgacgt ccgtcagcgc catcggctcc
    14821 cagtaaccaa tggccagccc gatacgcctg aacagcggcg gcggtgccgg acaaatctga
    14881 atgggcatcg acgggttcgc cgtgtcgggg accttaccct gactgacttt gatactgccc
    14941 agcgagagcg ggaaaataca gctccagcag atatccgtga tcgggtttac aaaacgcccc
    15001 tcacaggcag aatccgcaga tgccgcaggg acatgaccgg cgagaaggaa taacatcagc
    15061 agccacaggc ttcgcttcat tttctgccct cctctgccgg aataaattcc accttcagga
    15121 agcgatcgcc gggaacggcg ctcacccgtg ccgggacctg atcaatgccg aggcgctggc
    15181 agagcacacc gttctggtca aagtaaacac ggctgtccag agacttctgc atctccggga
    15241 tactgccctg cacgaggata attttgctct ccagtgtggg cggcgtctgg cgtttcatcc
    15301 aggccacctg cgccggatca tcgccattga tgaaatacag cgtctggtta aaaggcacat
    15361 actgcagcgg attcatcact tcaccctgac gggcaaacac ccgcccttcg ttatcccgga
    15421 tatccgcagc cagtctgacg gacgggtcaa acaaccggct gctgtatttc tccgtgcgtc
    15481 cgataccggg cacggcaggg ggccgcagac tgttgcggat cacccgctcc ttaaaggcat
    15541 ccattttccg gcccatctca ccggactgct ccagtgccgt caggcgctgc atgatcaccg
    15601 tcagcatgtc cggctccttt accggccaca gatcgcccca ggtgccaaga tcggcggcgg
    15661 ccacactctg cccccatatc agcagggcaa tcagcccccg gcatctcatg gttccgctct
    15721 cattcgccgg gcaatatcct gctggatttc ccgggtgata tccggagccc cctgtaccac
    15781 ggcaggcgac accaggatga ccgcgtggtg tttctgctgc cacgcctgca ggctggcttc
    15841 cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt gcttctgaca gttgtttctg
    15901 actggcgctg tcaaaaaacg catccacggt ctgtttcatg ttgaaggcgg cagtcaccgg
    15961 tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc agtaacacca ttgccaggca
    16021 tcccggcacc gtccaccacc agtggctgcg tcggggggct gtaacatccg tcgttttttg
    16081 cgttgtggtc atgcaacact cctgtatttc tcatgttctt ccagccaggc ttccagcgaa
    16141 gccatctccg gccctgactt cttccacgcc agctgccaca ccgcctcatg aatactcagc
    16201 ccctctttac gtttctgctg cacaaactca aaatccgggc cgtcagagct gtacatggcg
    16261 cggcttaacg ggtccacaaa caggcggtgc caggaggaat ggttttccac ctgcagcagg
    16321 aaggaactga accactggtc tttggccgca ccaaacttac caatcatgtc gcgctgcagt
    16381 ggctggaact ggtccggata cagctggttg tattttgcga actccttcgc actctgtttg
    16441 aggataattt tgtaggagga gttaccccat gccgcacggg ccgcactgga ggccttgtca
    16501 gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg acggcgggcg
    16561 gtacggtaac ctttctcaat aaactcaccg accttgcgat ttttgaagtc cagcagacgc
    16621 cagccttcat caataacgtt cagtttcttg aggttacgcg gcgtgcggta catcctgttc
    16681 tcgatgtaga taatcaggga gaacatcacc gcaaccagca gtgacggacg gtcttccagt
    16741 ccgcccagct ccagcaccac cattttcgca tcatcccgca gggacggctc atcggagtta
    16801 aaatactggc cgtaagtgcc gttggcagtg tactggtcaa gcagcacaat catttcgtcc
    16861 agacggctgc ggatggtcgg tgacccggca tactgctcgc tgtcactggc gtttttcagg
    16921 aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccagga ggccctgacc
    16981 gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct ggccatcact
    17041 gacaactggt cacggacacg ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg
    17101 ttaaaacgca gggtttcacc gtccagatat acgccaccca tgttctcaca cagggacttg
    17161 tagccatccc ccatgtcgaa caccacggca aagcccccgg agtccagcac gctgcggata
    17221 agtggctgta tcagcccggt tttaccggcc ccggaggtgc cacagaccgc catgttgtag
    17281 ttggtgttat tcatcccccg gaagaaaata tcgataaacg ccagctggtt acggtaggtg
    17341 ggtgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat caggttggca
    17401 acattaaagc tctctgcgcg ctgaaccact ccggcctctt tcagctgttt aaacaaccct
    17461 ttcccggcca taaagggcag acaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa
    17521 atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac ttccagcgcc
    17581 gtttcattgt tgtccttgca gaatgctgtg atgttgagga agtaggacac gacagaagac
    17641 tgaccggagc ccagacgctg acgcagttcc ccccactcct tcgcctcttt ctccacggac
    17701 ggaaaccatt tggcatagga ggtcttcgac tttttctcca ggtccatgta cttcaggttg
    17761 gcttcgctgt gggttttcac ctggtcttcc accaccagag tcagcgtcag gatgaacgga
    17821 caggagatgg acagttccgg gttcagcagg ttgctgtagt tgtcggccat attccacagg
    17881 aaggcgattt ccgggttacg ggccagatgg aaattcagga tacgggccgt gctgttcctg
    17941 ccgttctcac gcaggcccag cgtcaggtaa tcagcccgga cattcaggtc aaaactgtcc
    18001 tccacacact gataattcag atcagaatac gggtccagct gacgtctttt cgggtacagg
    18061 gagtccgggt tatggttaat catctccccg acaatatcga taaaggcctg tgcatccacc
    18121 gtctgtgtgg cgatactggc cccctgtaac gacgcccgga tgattttcac caggttttcc
    18181 atttccagaa tgtcagcccg gctttttttc ttcgagggag aacagtacga gataaatacc
    18241 cggtaatggc gcagggtcag gggcagattc atcccctccg gcagcggaaa ctgtgtcgct
    18301 gccgctttca tataataggc ccgggtaatg gcgttaaacc gttcagcctg ttcacctgac
    18361 caggagaact cacgcagccc gtactcaatc ctgtcaccaa ccagctgact ggacatcaga
    18421 tggatacaca acggaatacc gcgcggcagt ttggtgcgca gcatgtgatc gagtgcctcc
    18481 acaatggact tattcgcccc attgatggga atggcttcca gcataaagcc catcgtggtg
    18541 tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag acggctgaac
    18601 tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc aggcagtttc
    18661 agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt attactcata
    18721 tgagtattcc ttagtgcgtt taaaaaagga aaatatcagt ctgtggccgt ggatatatca
    18781 cgcagtgcca ttcctgaaac cacagcacaa ataagagatg gaggctttta acaggaaacc
    18841 aggattaacg ttcctttact gaatgagcat catttaatgc ctgaatttgt ttttcgcgaa
    18901 tcttattgcg ctcattttct cttacacgtt tcacctctgc catttcgttt ctttttctgc
    18961 attctgatgc gattttattt gccttaccaa gtgttaaagg ttcagactga taattaagtg
    19021 tctcctgaaa tgcttctgct gcacagggag tttcctgtaa aatctctgtc agttgttctg
    19081 gtgttgcagg ttgttgcgca aggtaaacga tgtaaaaagg aatacataca atcatagcca
    19141 taacgccaac aaatataaaa gccagaacag attttaaata tatcgacttc atttcatttt
    19201 ccttcgaagc agtaagcact cattgcccac caccatttag tgtttccatc acgctgttca
    19261 aaggttcatt tgttttaact tcaatacact cccctgtatt tccaaagccc ctgccgccat
    19321 gtgcttcgat tgtccgaaga acctgcacgc atacgtccgt atttggcatg ggtgtaatat
    19381 tccagcttcc gccaccgtta atatttttta tcagcacaag aaaaataaag gtatgcattc
    19441 tttattatct ccgcttgtat attaatcact gaatatccct tcagttaact tatgaggtca
    19501 taaaacactg cagattctca ttcggattta ttcccttcag taaacttccg aatagtatcc
    19561 tgttcacgct gacgggtggc agactcctgc attgcatcca ctttctcctg ccagagacgc
    19621 cggatatcct gttcatcccg accagtcagt tttttaccgg acaacaaacg gttaagcagt
    19681 tcctgtctca catctggtac gtcttcagtc agacgcataa gccggagtaa ttcctgacga
    19741 ctgagtacca tgcgtgattc ctccggtacg ctgtaataat taaaaatacg actgaatacc
    19801 ccacaacagc aggcaacgga caggcacagg gatttcagat acaggccaga aacacagaat
    19861 gtggcaacac cacaaagaat agctataact aaaaatccct tacccagtgc catacacaaa
    19921 gaaccctgtt ttctggatat ccgctcatgc tcatccagaa taaaatctat tttttcattt
    19981 tctgatttat gcatagtgtt tcctctgcct ttcctgatac gcctgacatt caacgcacaa
    20041 cgtcacaccg ggaaatattt tccgccgggc ttccggaata ggatttccgc atgcttcaca
    20101 gagataaaca ggaataccat gaacatttat ttcctggcgt atccggttta ttcctgtcat
    20161 ggtcagttgt tctgtcactg aatatgcttc atcggcttca tcactcacgg aatattcctc
    20221 ccgacatgtt ctgacacgct accgcccatc ccttttcacg cacagggcac tcgttttaca
    20281 ggcggatttt gaatacagaa agcccgccgg cattgccggc tgatatacaa cttcagggcc
    20341 agttaattaa tacgtggttt tccccacgca gacggtttaa taacaaaaaa cacgctggat
    20401 ggctgatgat aaacatcctg gttatcaata taaggcgcta tccataatgc tgccgtctgc
    20461 tcacccgttc ttagcggacg aggaggtgtt accggcgtaa ctgaactgac cggaacaacc
    20521 gttttcactt cccgggcagc ggtaaacagc gggcgtggtg ccagcagttt ctgctccgga
    20581 tgtgctgtca ccgcaggtct gctgccggac ggagtggtcg cggtgacggt ctgcaccggc
    20641 attgtccgga agttcccttc agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct
    20701 gaggactgct ccagtttttt ggccttctca ttggcctgct ccatcgtcat acaggtatcg
    20761 gatgtggtgg cgttacactc aaattccgta tttgttcctg cgcagccgga aagtaataag
    20821 gttcccagca gaggaataaa taaagaaatc tgtttcattg cccttctcct tttttattac
    20881 tccccacaac agcagaaatt aactctgctg tcagatttgt tttccctgca cagggatgtc
    20941 cggagaccac tgtatttccc ggagaagaaa ataagctcat cgacattccc ttcttcatca
    21001 caccgacagc agccatatta aaattgtgcg gcaatgattc agaactgaac accttcggag
    21061 caggaagaac tatttttgtg gtgcagttac tgagaacagc atgcataaaa tcatctgata
    21121 cgtttttatc cggcacaacg cgggctgtaa taacattaat attacgcatg ttcattttta
    21181 tctccgttgc ttctgtttgt tcggtcatca gggaaataaa catggcattt cctgattttt
    21241 cccggcaggg aagcgtcttc cgtataaacg tccttcagat attcatctgc ggttaccacc
    21301 cggatacatt tcatttttcc gggcagcacc ggatatttcc cggtgcctgc cgcaaacgac
    21361 atatacatca gtgccagcgt tgcccagaat gcggtgacca gttgcatcag tgaccggtca
    21421 gtctccctga cataatctgc agcatcagcc gcgcggtaat ggcagatatc ctgtatttct
    21481 gatctgcctg aagaatcaga ggaagaaaac cagcattcac tgaccgggaa ctgtccggca
    21541 ggtgctgttt gtcttacgaa gttaatctcc ttaaccagta atttccccgg cgtggtgttg
    21601 tccttccata aaacaaacag agccatcagg gccattgtgg ccagcggcca gataaccaca
    21661 aacctgacga cccacagcag ccagcgaacg acaaacgcca cacggaatgt cagtgcatca
    21721 cccgccattt ccgccctcct tgccggcatc cccgcccagc gggttcttgt ctccggacat
    21781 aaactgctgc ggattcagat tgcccagctg tttcagcatc tggtcggtgt taaaaccgtt
    21841 caggtggctt tctgccgagg gaggattcgg caccgggctt tccgggttac cctcttccgc
    21901 tgtactctgt gactgctcca gtgccatttc ctccacggtt ttcagctgga agccgtcctg
    21961 gaacaccacg gtcacttcgt tgcccgcacc aatcggtatc accgggtgat actgttcggc
    22021 acgtttgatg tagtagtcac tgagcgtctg tgcggctttc gatgcgccgc caccgatacc
    22081 cattttcagg acatcaccag ccccgtaagc ggctgtggca cccagcccga cagccggttg
    22141 ggaggcgcgc tccattccct gaccgatacc gtcaacaaat cccgcgcccc atgcccagcc
    22201 gaggattttg ccgttacgca tcaccacttc gcccttgata ccgtttttac cccggaagct
    22261 gacatgcccc ttaatcggca tatcaatagt tttgccgtcc ttcaggcaac tgatattgcg
    22321 ggtgcgtaca atggcacgtt cactggacac atccccccag gcttccagcc ccacaaaaca
    22381 tcccgttgcg tcatacgtct tgctgttcgg catttccacc aggccggtga tacgcaactg
    22441 catcggcacc gtggattcat taccggtgac tgaggcattg gcatccgccc cttcaatcag
    22501 catggctttc gcaaaacttc ctgacggaat gtacggcagc gatggtccct gttttccctc
    22561 attacgggta aacaccttac gctgtatccg gttaggcacc ggcacagact ggtacgtcac
    22621 ctgtggtggt ggcgtgacac cattcccggg ataaaacgcc gtcggcggtg gaacagcaac
    22681 gctgccctgc ggcgggaagg atacgggggt gtttcctggc tgtggttcgc cttccgggcc
    22741 gggcggtggg gcaggcatct gcggtacagg ctcaccggtc gccgtgacgg gattagcacc
    22801 cagggctttt acctgctctg ccagggcggc attgtcctgt cccagctttt caatacgacg
    22861 ctggtcatca ccgcgctgtt tgttcagaac atccagctca cgacggattt cctcatactg
    22921 cttctgcatc tgcgctgccg tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc
    22981 atcaaaggtc gtatccacca caccggtcat atccggcaca ggctcctgtt cagccacggt
    23041 ttcaccgtta ccggacatgt ccacatcaga cagatacagt gccccaccaa tcgcggaggc
    23101 tgtaccgaca accacaatcc ccagccacag gtactgcttg cgtttcacaa tggtattgat
    23161 actggccatc agttgccctc cccgtcacgg atgacataca catccatgcg cgcacctgcc
    23221 agtaactggc tggcaggctg gctgaacatc acggcacggg ttcccggctg ccagaaatca
    23281 ctttcccgga tattcagggc agacagcgtt ttattttcca cgacaaagcg gaccatcttc
    23341 aggtgattcc ccgtccatac ggaatctgcc actgaagaca gccccgccgg ggcctgcagg
    23401 gtttcctttg tcacaggaac ctgataccag cctgcaggta attttccgcc acggacagcc
    23461 tggctgatgg tcacaagcag ggattcgtaa ggcgtggacg tttcccacgc tccggcttct
    23521 tctccggttc cgcgcaggtc actgaccagc tgaatggtac gccccgcgcc ttcacgggga
    23581 acagcctgaa tggaaaggtt cagaccacgc tctgtttcca gaatgaacgt aaagggcttt
    23641 ttgttgacgg tggccaccac cacaccgccg gaggcggtct gctcattatt ggtcagggca
    23701 ccatccagac tgttcacggc gataatccgg tcaccgggaa cggtaaacag attcgggctg
    23761 gtattgctga tggcaatact ggcctgacca ccgttcacca ttggcaccac agtgggggcc
    23821 agcgtaccgt ttgcggccat cacgctgcag gaaaaaaaca ggccgccgaa tattattgcc
    23881 gtattatttt ttctcatcgt ctgtttcccc gaaattatcc agccaggtca ccccgttttc
    23941 ccgcttcaga ataaggatgt aatgtttgat atcagtaaaa ggttcggagt taccaatcca
    24001 tgttttccga attccacgga tttccacacg accatactga ggccagacac ggacactggt
    24061 ctggaaaaaa gcagaattca cgttatcttt tttaatgagt ttcgcttctt cagccagaat
    24121 aactttcatc tggttctgtg cacccgggcg gatatattgc agaagcgcct gatgtgaagc
    24181 atcgacgatt tccggtgaga cattcagacg gagggcaata aatgacagcg ccatctgctg
    24241 caaataagag gcatcggcac tgttctgtga cacggcaaag ggcacattaa atcccattgg
    24301 ggtcacggca gtgcgttgct cattctgaag acggtagtta ttcaccccct gaatgaggtt
    24361 aacagagagg ctgagaacaa taagcactga cataaatata aaggcgatgg ccattacacg
    24421 actggtactt aaacgggcac cgtgttccat ataacaatcc tggtatcagt tctatttaat
    24481 ccactgccgg aaacacgaat cgggaacatt atgaaaaata ccgcgcagca gggctgttgg
    24541 catataccag taaatcaggt cacgtaacca ggaactgccc cgcccttttt tcagtttttt
    24601 aatcccgaaa taaaccagaa ccgctgcacc aataccgaac agatatttcg atgttgtgat
    24661 accccagcca acacagattg ctgcggggat cagttcatcc agtggcaggc caaaccagcg
    24721 gctctggtt
//
LOCUS       DAZFDP010000062        24524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFDP010000062 DAZFDP010000000
VERSION     DAZFDP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            185..1390
                     /gene="gltS"
                     /locus_tag="ACHJ1G_002557"
     CDS             185..1390
                     /gene="gltS"
                     /locus_tag="ACHJ1G_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4764397.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1393..2259
                     /locus_tag="ACHJ1G_002558"
     CDS             1393..2259
                     /locus_tag="ACHJ1G_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764398.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2244..4325)
                     /gene="recG"
                     /locus_tag="ACHJ1G_002559"
     CDS             complement(2244..4325)
                     /gene="recG"
                     /locus_tag="ACHJ1G_002559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4764399.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4331..5020)
                     /gene="trmH"
                     /locus_tag="ACHJ1G_002560"
     CDS             complement(4331..5020)
                     /gene="trmH"
                     /locus_tag="ACHJ1G_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4764400.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(5027..7135)
                     /gene="spoT"
                     /locus_tag="ACHJ1G_002561"
     CDS             complement(5027..7135)
                     /gene="spoT"
                     /locus_tag="ACHJ1G_002561"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4764401.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7154..7429)
                     /gene="rpoZ"
                     /locus_tag="ACHJ1G_002562"
     CDS             complement(7154..7429)
                     /gene="rpoZ"
                     /locus_tag="ACHJ1G_002562"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4764402.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7484..8107)
                     /gene="gmk"
                     /locus_tag="ACHJ1G_002563"
     CDS             complement(7484..8107)
                     /gene="gmk"
                     /locus_tag="ACHJ1G_002563"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4764403.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8365..10047
                     /gene="ligB"
                     /locus_tag="ACHJ1G_002564"
     CDS             8365..10047
                     /gene="ligB"
                     /locus_tag="ACHJ1G_002564"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4764404.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10044..10661)
                     /locus_tag="ACHJ1G_002565"
     CDS             complement(10044..10661)
                     /locus_tag="ACHJ1G_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4764405.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10953..11777)
                     /gene="dinD"
                     /locus_tag="ACHJ1G_002566"
     CDS             complement(10953..11777)
                     /gene="dinD"
                     /locus_tag="ACHJ1G_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4764406.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11998..12861)
                     /locus_tag="ACHJ1G_002567"
     CDS             complement(11998..12861)
                     /locus_tag="ACHJ1G_002567"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4764407.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12988..13704
                     /gene="rph"
                     /locus_tag="ACHJ1G_002568"
     CDS             12988..13704
                     /gene="rph"
                     /locus_tag="ACHJ1G_002568"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4764408.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13770..14411
                     /gene="pyrE"
                     /locus_tag="ACHJ1G_002569"
     CDS             13770..14411
                     /gene="pyrE"
                     /locus_tag="ACHJ1G_002569"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4764409.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14448..15044)
                     /gene="slmA"
                     /locus_tag="ACHJ1G_002570"
     CDS             complement(14448..15044)
                     /gene="slmA"
                     /locus_tag="ACHJ1G_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4764410.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15151..15609)
                     /gene="dut"
                     /locus_tag="ACHJ1G_002571"
     CDS             complement(15151..15609)
                     /gene="dut"
                     /locus_tag="ACHJ1G_002571"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4764411.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15587..16807)
                     /gene="coaBC"
                     /locus_tag="ACHJ1G_002572"
     CDS             complement(15587..16807)
                     /gene="coaBC"
                     /locus_tag="ACHJ1G_002572"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4764412.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16979..17647
                     /gene="radC"
                     /locus_tag="ACHJ1G_002573"
     CDS             16979..17647
                     /gene="radC"
                     /locus_tag="ACHJ1G_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4764413.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17864..18100
                     /gene="rpmB"
                     /locus_tag="ACHJ1G_002574"
     CDS             17864..18100
                     /gene="rpmB"
                     /locus_tag="ACHJ1G_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4764414.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18121..18288
                     /gene="rpmG"
                     /locus_tag="ACHJ1G_002575"
     CDS             18121..18288
                     /gene="rpmG"
                     /locus_tag="ACHJ1G_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4764415.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18386..19195
                     /gene="mutM"
                     /locus_tag="ACHJ1G_002576"
     CDS             18386..19195
                     /gene="mutM"
                     /locus_tag="ACHJ1G_002576"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4764416.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19234..19713)
                     /gene="coaD"
                     /locus_tag="ACHJ1G_002577"
     CDS             complement(19234..19713)
                     /gene="coaD"
                     /locus_tag="ACHJ1G_002577"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4764417.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19721..20998)
                     /gene="waaA"
                     /locus_tag="ACHJ1G_002578"
     CDS             complement(19721..20998)
                     /gene="waaA"
                     /locus_tag="ACHJ1G_002578"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4764418.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21411..22469
                     /gene="rfaQ"
                     /locus_tag="ACHJ1G_002579"
     CDS             21411..22469
                     /gene="rfaQ"
                     /locus_tag="ACHJ1G_002579"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4764419.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22466..23590
                     /locus_tag="ACHJ1G_002580"
     CDS             22466..23590
                     /locus_tag="ACHJ1G_002580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4764420.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            23583..24380
                     /gene="rfaP"
                     /locus_tag="ACHJ1G_002581"
     CDS             23583..24380
                     /gene="rfaP"
                     /locus_tag="ACHJ1G_002581"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4764421.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5751 a         6055 c         6600 g         6118 t
ORIGIN      
        1 taaatgcact tttttatata acttttggta tttttctgct taaaatccat gccatttgtt
       61 cattaacagc tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga
      121 agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt
      181 aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct
      241 cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt
      301 tgcgggtggt ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga
      361 agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg
      421 cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat
      481 tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt
      541 agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg
      601 tacgggcgct gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga
      661 agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt
      721 ggcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt
      781 cccgacggcg tttgaaaagc cggatgtggg acgcatgatc acctcgctgg tgctgattga
      841 aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc
      901 tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag
      961 caacggtctg tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg
     1021 taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac tgtgggagct
     1081 ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt
     1141 gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc
     1201 tgcgggtcac tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc
     1261 gatcactgaa cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc
     1321 gttctttatc gatatcgtca atgcgctggt gattaagctg tatttaatgt tgccgatttt
     1381 tgccggttaa aaatgaaaaa gcgtcggaag gaaccggaaa cgctacgtga acactgtcgc
     1441 catatttttg gtgatgagcc gccagtgctc tgtgtatggg aaacggaatt tgattacgca
     1501 gatgccgaac tcaaggcgct ggcggctaaa gagtggcagc agatcagcga atgggatttg
     1561 agtgcttact atgtactcaa tctcgtttat aacgaaccga tgcaaataga gttgtttcgc
     1621 tatctgtttc cgctctgcct ggcgcaatgg catgaaaccg ttttagctgg cggttatggt
     1681 gatcacttcg aagaatcctt aatgaaggca ttgtgtcggc cctatctctg gcaggagatg
     1741 atgaatgctt cccaacgcca gcaggtacgg caatttttac tggacaccgc gttgcagcgt
     1801 atggataacg agcgtggttt caataacgtt ctttgctggc tggcggtgtt caatactctg
     1861 ggtggggccg cgccgttaat tcgttctctc tggtcgcgct ggtgggcgct ggatacgcca
     1921 ggaaaagcgg tgtgtgctat tcagtatgcc gcgcatctaa tttatcccat agaagccaat
     1981 ccgttatggt cgcaagaatg gataggctgg gggcatccat taggccataa agatggctgg
     2041 tcctctgata atcgcgcttt tcttcgccaa atgttaacac ctgaaatgat tgtggctggc
     2101 gtacaggcag cggctgaaat tctgcgtggt gaaccggaag gcgcaatggc ggcgcgaatt
     2161 gcacaggatg cgtatgaggc aatggatatc ctcactatcc agattgaaga tttactgcgt
     2221 gacttgtctt gtgatgagtc tggtcatgcg ttggagtaac gttccgtctc cggcatccag
     2281 cgttctatca gggcttttgc ttgttgtggg taacgttcgt gaatatgtcg cgccaggcgc
     2341 tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ctcagcatta
     2401 cccgtctgac gcgtacctaa cagttcaccc ggtccgcgaa tctctaagtc tttctgtgca
     2461 atcacaaaac cgtcgttgct gtcgcgcagc acttgcagcc gaatttgcgc ggttttggag
     2521 agcggcgttt tgtagagcag cacgcagtga gaagccaccg caccacgacc tacacgcccg
     2581 cgaagctggt gtaactgcgc cagacccaga cgctccgggt tttcgataat catcagactg
     2641 gcgttaggca catcaacgcc gacttcaata acggttgtgg caaccagcag gtgtagctca
     2701 ccttgtttaa acgacgccat caccgcctgt ttctcggcag gtttcatccg cccgtgtacc
     2761 aggccaacgt tcaactctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc
     2821 tgcgcttcca gcaattccga ctcttcaatc aacgtacaaa cccagtatgc ctgacgacct
     2881 tcagttatgc aggcgtggtg cacgcggtca atgatgtcgg tacggcgggt atcaggaata
     2941 gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga
     3001 tcggcatacg cagtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga
     3061 tgcggatgga agccctgctg ctggcctttc tcccacaatg ccagacgctg atgcacgcca
     3121 aaacgatgct gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg
     3181 aagatggcgt gtgtaccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctgt
     3241 gccagccgtg ctttaccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt
     3301 gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga gtaattcggt tggtgccatc
     3361 aatgctacct gtttgccgtg ggcaatcgca cgcaacgcgg cgagggcggc gaccagcgtt
     3421 ttaccggaac ctacatcgcc ctgcaccaga cgcatcatcg gcacatccag cgccatatcg
     3481 cgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg
     3541 aggagtttat ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa acgctgtgct
     3601 ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga
     3661 cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg
     3721 tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg
     3781 agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc
     3841 gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct
     3901 ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta
     3961 ccgcgctttg cttcgccata agccagtaca cggcggcccg ccgccaggct atttttcatt
     4021 gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag
     4081 gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg
     4141 gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc
     4201 aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta
     4261 ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct
     4321 ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct
     4381 cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca
     4441 ccggatagcc gccttcaaac aacaggcgtt gttgctctgc ttccggcaac atgctgtttt
     4501 cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg
     4561 aggcaacgga cacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat
     4621 ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg
     4681 tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga tgggttgcca
     4741 gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt
     4801 tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac
     4861 caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag
     4921 aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg
     4981 cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc
     5041 gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca
     5101 cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg
     5161 tattcaaact ttgaatattc gaggtcgtgg tgttaattgc cgccgtcagg tttgccagcg
     5221 caccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt
     5281 tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg atgttacggc
     5341 aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc
     5401 caggaatggg gcggcagcat ttcgcaaagg tgatcagcac gccatcagcg cctttgatcg
     5461 gcagatgccc gtggctttga gtcgccggtg gaatggaggc gtccccatgt tgtagatttt
     5521 tcgcgaccac cacgctcatt gcgttaccaa ggccgatttc tgccagcaga tcgtcaagcg
     5581 ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattgagct
     5641 tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac
     5701 gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt
     5761 tcagccaagc ggcattcggg cgagcgcccg gagcggtaat gatttcaacg gtctgaccgc
     5821 tagtaagcgg ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc acgcaggcat
     5881 ggccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct
     5941 cgacaatgcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa
     6001 cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca
     6061 tccagcgctg ggcgcggatt tgcgcggtgg tgctggtttc gccgtgctct ttataagccc
     6121 agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct
     6181 caaccggcac accgtgcggg ccgatcatcg aggtgtgcaa agactgatag ccgttcgctt
     6241 ttggaatggc gatgtagtct ttcacgcgac ccggacgcgg cttgtacagg ctatgcattt
     6301 gcccgagcac gcgataacag gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt
     6361 ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat
     6421 gcttctcgcg accactgacg cggcacggaa ttcccgcttc ctgcaaacgc ccttcgattt
     6481 cagagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc accacttctt
     6541 taattacgcg gtaacgattg ggatacagcg cctcaaaacc cagctcttcg agttcggttt
     6601 taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg
     6661 cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt
     6721 cggcaagttt gatgaggatg acgcggatat cctgcaccat tgccataatc atcttgcgaa
     6781 agttttcggc ctgcgcctct ttcttatcac ggaacttgag tttatcaagt ttcgacaccc
     6841 cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg
     6901 gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca
     6961 tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac
     7021 gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt
     7081 ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg
     7141 acccgctttg tgattaacga cgaccttcag caatagcggt aacggcttgt aattcagcgg
     7201 cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac
     7261 cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg
     7321 gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa
     7381 aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc
     7441 tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt
     7501 tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa
     7561 taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc agataatcat
     7621 attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt
     7681 cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa
     7741 taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga
     7801 tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc
     7861 catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt
     7921 taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt
     7981 ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag
     8041 cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt
     8101 gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat
     8161 acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt
     8221 ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag
     8281 cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc
     8341 agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg
     8401 tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa
     8461 gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga
     8521 aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa
     8581 cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg
     8641 catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg
     8701 atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg
     8761 gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag
     8821 gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta
     8881 gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa
     8941 atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggacgatagc
     9001 gacacgctga attctctggg cgtttttgtc tgggcatggc cggatggacc gcagttaatg
     9061 accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt
     9121 gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta
     9181 cccttcgtca ccgatggcgt ggttgtgcga ggggcgaaag aacccgaatc ccgccattgg
     9241 ctaccaggcc aggcagaatg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc
     9301 gaagtgaagg cgattcagtt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca
     9361 ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg
     9421 cgctggcagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag
     9481 ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca
     9541 ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag
     9601 ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga tggtattggt
     9661 gaggccggct ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt
     9721 ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag
     9781 ctatggcatc ggtttaatct ggctcgtaag cagcctttta ctcgctgggt gatggcaatg
     9841 ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt
     9901 ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatcggg gcgcgccaga
     9961 caggttattg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc
    10021 cagcagatca caggttttga accttagtgt ccttcgtggc tgtagtaaaa gaccggtaat
    10081 cccagcttaa ggcgtagcgc cagtaagcgg gcaaaaaagc cgaataccag ggtggagata
    10141 attaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca
    10201 aatgagacac cggcatacaa ctctttctgg aataccagtg ggatacgttt acagaacata
    10261 tcgcgcaaaa cgccgccaaa cacgccggtg gttaccgccg cgacaacggc aataattggg
    10321 ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccaagc
    10381 gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg
    10441 agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga
    10501 tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggtcgcaata
    10561 ataattacgc caaatgtatc catgcggcgt cgcccggccg ccagagcccc cgtcatggct
    10621 tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc
    10681 tgtctggcag ggtagcgatt tgtgcgtatt gtcacgattg agatttttta aggtgagagg
    10741 aaattgataa cttgtagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct
    10801 gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaattgaggg gtggaaaaga
    10861 aaggtgcaat gtttagccat tgtgttcacc gcacctggac tttggcttac gcatcgggta
    10921 tctcaatcaa caaaaagagt ttttagtttc tgctatttct tctctgtaat ttttacactg
    10981 ttttccaact gcttgatgct gacctgcggg gtcggcaact cctcaggcat ggttccgcca
    11041 agttcctgaa tggtttgcct caccttgcta cccacgtcaa agtgtgtggt attagcttgc
    11101 tgttttgaat tcacgccatc ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga
    11161 ttagccgcca gttctgttga acccatatga tccaggatct tctgactctt tttcagcccc
    11221 ttccgctgat ggatggcttt ctgatctaat ccaccataca gcccccggta accatgattc
    11281 tgaaatatgg cgaaatcgat agctgtcgct accccagctt gctgcgctgc ctcaaccaat
    11341 tgtttgttat gttctttcaa ttcatttctc agaaaaagac gcttttcgtc ttcacgaagt
    11401 tgcctgaaag cttcatcgtc agccagctcc tgccgtcggg tctggatagc aaaataagtt
    11461 tgccctgccg caataaccgg tttcgcaggg tcgccgtttt gtactaccag gtaacaggca
    11521 tagcgggaga gatgaacatc ttcaagttct cgttgagccc ctgaacctaa tacaaccatt
    11581 ttggtggttt caacgaaatg gtcagatgca gcttgattgc tggcttcgca cgcttgagtc
    11641 gcgcgggcca gtaccttttg aaaattgcgc cagtcccgat aatccagtaa cggtgccaat
    11701 tctctagctg accaaaattc ttgcccttca gtgccataat gccgtatttc ttcaaaaggt
    11761 tgatgatgtt cgttcattgc ccactccatg tgatatcgat tgcaaagaaa gtaaatctgt
    11821 aactgtattt atatacagtt gtgagtatct cacgttctca gtgtgctggg caaaaaaaca
    11881 tcgcaaaatc tggagcaggc acgcaaaaca gaattgaagt tgcggggaag aaaaatgccg
    11941 ctctgtttga gtgggcggca tttgtgaagg ctgaaaagta agaattagtg ttacgagtta
    12001 ctcaatattc tgaatctgct cgcgcatctg ctcaatcaac actttcagct caatcgcgga
    12061 gtttgtcact tcggcattga tagacttcga cgcaagagtg ttcgactcgc ggttgaattc
    12121 ctgcatcata aagtccagac gacgaccaac ggcttctttt ttcttcagaa tgttgtaggt
    12181 ctctttgaca tgcgcttcca ggcggtccag ttcttcggca acgtcaattc gttgtgccag
    12241 cagaaccaac tcctgctcca ggcggttgtt ttccagttgc acttgagcat cttccagctt
    12301 cgcgaccaga cgctcacgct gccattgcag gatttccggc atatgggcgc ggactttgac
    12361 cacttcggcg gtgacgcctt ccagacgctg ctcgatcaat gctttcagcg cctgaccttc
    12421 ggtttcacgt gcgacgataa agtcgtccag ggtaccatcc aacgccgcga gaatttcagc
    12481 ggcaatcgcg tcgagatcct gctcctgggc tgccattacg cccggccagc gcagaatatc
    12541 aaccgggttg atttcccctt cgtcactctg catttttacc cagttcgcgg cagttaccag
    12601 ctgtttagcc agtttttcgt tgaggatcag ctcaccttgc gcgctaacat ctggctcata
    12661 gcgcagggta cattccactt taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac
    12721 aggttcaagg ctacggaact gctccggcag acgaaagtaa gtttccagat aacgctggtt
    12781 taccgagcgc atttcccagg ttgcgctccc ccattcaccc ttgatttcac gccgggcgta
    12841 ggcggtcata ctgcggatca tagacgttcc tgtttataaa aggagaggtg gaaggattat
    12901 agccatcgat gccttgtcag gataggaata accgccggaa gtccgtataa tgcgcagcca
    12961 catttgtttc aagccggaga tttcaatatg cgtccagcag gccgtagcaa taatcaggtg
    13021 cgtcccgtta ccctgactcg taactataca aaacatgcag aaggctcggt gctggtcgaa
    13081 tttggcgata ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg
    13141 aaaggtcagg gccagggctg gatcaccgca gagtacggca tgctgccacg ttctacccac
    13201 acccgtaacg ctcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat ggaaatccag
    13261 cgtctgatcg cccgtgctct tcgcgcggca gtagatttga aagcgctggg tgagttcacc
    13321 attacgctgg actgcgacgt gcttcaggct gatggtggca cgcgtaccgc gtcgattacg
    13381 ggtgcctgcg tggcgctggc agatgcgcta cagaagctgg tggaaaacgg caagctgaaa
    13441 accaacccga tgaaagggat ggtagccgca gtttctgtcg gtattgtaaa cggtgaagca
    13501 atttgcgatc tggaatacgt tgaagactca gccgcagaga ccgacatgaa cgtagtgatg
    13561 accgaagacg ggcgcatcat tgaagtgcag gggacggcag aaggcgagcc gttcacccat
    13621 gaagagctac tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat tgtagcgacg
    13681 cagaaggcgg cgctggcaaa ctgattttta aggcgactga tgagtcgcct tttttttgtc
    13741 tgtagaaaag taagatgagg agcgaaggca tgaaaccata tcagcgccag tttattgaat
    13801 ttgcgcttgg caagcaggtg ttaaagtttg gcgagtttac gttgaagtcc gggcgcaaaa
    13861 gcccctattt cttcaacgcc gggctgttta ataccgggcg cgatctggca ctgttaggcc
    13921 gtttttacgc tgaagcgttg gtggattccg gccttgagtt cgatctgctg tttggccctg
    13981 cttacaaagg gatcccgatt gccaccacta ccgccgtggc gctggcggag catcatgacc
    14041 ttgacctgcc gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa ggcggcaatc
    14101 tggttggtag cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc accgccggaa
    14161 cggcgattcg cgaatcgatg gagattattc aggctaatgg cgcgacgctt gctggcgtgt
    14221 tgatttcgct tgatcgtcag gaacgggggc gcggcgagat ttcggcgatt caggaagttg
    14281 agcgtgatta caactgcaaa gtgatctcta tcatcaccct gaaagacctg attgcttacc
    14341 tggaagagaa gccggaaatg gcggaacatc tggcggcggt taaggcctat cgcgaagagt
    14401 ttggcgttta aagaaactcg ccggatgaaa agtcatccgg cgtcatatta ctgcaactgt
    14461 gccgcaatta gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt aaattcgctg
    14521 cggacaaaac gtgacagcat accttcacag aaggccagga tctggcttgc cagcagggtt
    14581 tcatcggtgg tgtaaccttc accctcacgc attctctttt cacgcaatac ctggcgcagc
    14641 tgcgcttcaa tacgctcgaa cagctggttg atgcgccctt gcaggcgatc ctgttcaaac
    14701 attagcgcat gaccagtgag gatgcgggtc aggccaggat tacgctcacc aaaaccgaga
    14761 agcagcaaca caatcagacg caggcgcgct gtggtgtctt tctcatcttt cagaatcagg
    14821 ttgatgcgag taatcaggct atcttcgata aactcaatca ggctatcgaa catgcgggtc
    14881 ttactgggga agtggcgata cagtgccgct tcggaaacgc cgacagaggc ggccagtttt
    14941 gccgtcgtga tacgttggct tccatcgctg gattccagca tcagcgccag agactgaagt
    15001 atttcctcgc gacggttcct tttcgcagtt tgtttttctg ccatgttaca aaatacccct
    15061 gaaaataagc acttgccagg cggcacccac gctatgaccg caaacgaaat gtttgcggct
    15121 atgttatgac gttattcgga tgcgtatgtg ttactgacga ccagagtgac caaagccgcc
    15181 ttcaccgcgg tcggtggcgt cgaaatcttc caccagatta aattcagcct gtactaccgg
    15241 aacaaaaatc atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg
    15301 gttccacacg gaaatcatca actggccctg atagtcagaa tcgatcaatc ctaccaggtt
    15361 accaagcacg ataccgtgct tatgtcccaa tccggagcgc ggcagcatca ttgccgccag
    15421 tgaaggatcg gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt cacccggagc
    15481 cagttctacg gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc cagaggtggc
    15541 ataagtcggg agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt
    15601 tttcatcata acgggtcacg atctcgtcga gtaataattg gccaaggagc tctttgcgct
    15661 caagcggtaa gactttatct ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt
    15721 taaatccttg agttggctgg gaaacatcgt tcgcgcagat cagatcaagg tttttacgga
    15781 tacgtttttg ccgggcgtat tcttccacat tatttgtttc ggcggcaaat ccaacgacgt
    15841 agggtcgatg gtcttttagt gcggcaacgc ctgcgacgat atcggggttt ttaaccattt
    15901 ttattgttaa ttcatcaccc tgcgtggcct gctttttgat tttctctggg gccacggtag
    15961 ctgcgcgata atccgccacg gcggcgcagc cgataaaaat attttgctgc tgtacagaag
    16021 cattcacggc ggcttccatt tccagcgcag tcatcacatc aacacgttta acaaacggtg
    16081 gcgtcggtag tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg
    16141 cggcggcgat agcaaaaccc atcttgccgg agctgtgatt agagatataa cgcaccggat
    16201 cgagcggttc acgcgtcgga ccggcggtaa tcataatgtt cagatgtttc aggtcgttga
    16261 cgggcgaaaa atgcgccacc gccatatcca caatggttaa cggatcgagc attcgcccag
    16321 gaccgatatc accacaagcc tgactgccac tgtctggccc ccagatgagc aaaccacggg
    16381 aagcaagcac ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg
    16441 cggggagcac ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg
    16501 ccattccggc agcaacacgg gcaatcaaat ctgccgtggc aggggcgaga atcactaaat
    16561 cagcccattt acccagctca atatggccca tagcggcttc ggctgccggg tccagcaaac
    16621 tgtcggaaac gggataacca gaaaccgcct gcaagctaag tggggtgata aaggcttttg
    16681 ccgcttcggt catggctacg cggacgtcgg ccccgcgatc gcgcaaacga cgcaccagtt
    16741 caggggtttt ataggcagca ataccgccgc taacgccgag aacgattttt ttaccggcca
    16801 ggctcatgat gatttttcct gttgggtgac accagaagtt ggcgatttta tcacaatact
    16861 tttgttgtcg tgccttcacc tgagaatcac tttgcgaggc gctttccagg attgaaaact
    16921 ggccgtcgat ttaacggaac ggctatgaca ggatgcgagc accacaaagg aggtgaaggt
    16981 gaaaaacaat gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc
    17041 cttaacggat gtcgagctgc tggcgttatt tctgcgtacc ggaacgcgcg gtaaagatgt
    17101 attaaccctg gcaaaagaga tgctggagaa tttcggctct ctttatggct tgttaacctc
    17161 tgaatatgaa caatttagtg gcgttcatgg aattggcgta gcgaaatttg cccagttaaa
    17221 ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt
    17281 actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg aggagcggga
    17341 gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata gccgtctttt
    17401 ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg aagcgataaa
    17461 aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg ctgaacccag
    17521 taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg
    17581 cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg
    17641 gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc
    17701 gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc
    17761 aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata
    17821 ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca
    17881 agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa
    17941 acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg agaagcgttt
    18001 tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac
    18061 agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc
    18121 atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc
    18181 tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat
    18241 ccagttgttc gtcagcacgt gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga
    18301 tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc catattgatt
    18361 gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg
    18421 cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcagtggtgc gcaacggacg
    18481 cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt
    18541 gcagcggcgg gctaaatatt tgctgctgga gctgcctgag ggctggatta tcattcattt
    18601 ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca
    18661 tgtggatttg gtgatgagca acggtaaagt gctgcgctac accgatccgc gccgctttgg
    18721 tgcctggctg tggaccaaag agctggaagg gcataatgtg ctggcccatc ttggaccgga
    18781 gccgctcagc gacgatttca atggtgagta tctgcatcag aagtgcgcga agaaaaaaac
    18841 ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga atatctatgc
    18901 cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct
    18961 ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca
    19021 gggcggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct atttcgcaca
    19081 ggaattgcag gtttacgggc gaaaaggcga gccgtgtcgg gtgtgcggta cgccgattgt
    19141 ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattcat
    19201 gcgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca tcagcgcctg
    19261 atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac
    19321 caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac tttccagttc
    19381 cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat ccgccaccgc
    19441 acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc
    19501 actaaacccg accacttcca cgttccccag atgcgcggtt gcctgctgtg ccagcgccac
    19561 acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg ccagaataac
    19621 gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc
    19681 gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca
    19741 ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga tacagtactt
    19801 caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa gaggacacct
    19861 cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc tccagccgcg
    19921 cgcaaatgtc tttaaagtta aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg
    19981 cagcttccag cggattatgc ccaccacgtt caaccagtga accgccaaca aaggcgagat
    20041 cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc
    20101 tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc tggcggacaa
    20161 gattaatcgc atccgggaag cgttctggat gacggggtac caggatgagc aataaattcg
    20221 ggaattgctg taacaatgcc tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag
    20281 tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt
    20341 tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta acggtcacct
    20401 gattattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt tgcgcagcaa
    20461 tcagcgtaat acgacgcagc aagcgacgga cgaatttacc cagtttggca taacctgcgg
    20521 ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttgtgtagcg
    20581 ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg gggtcgactt
    20641 tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg tgctgaacat
    20701 ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa
    20761 tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc gccagagttt
    20821 caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac
    20881 cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc
    20941 gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag
    21001 taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt
    21061 acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata
    21121 ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga aggaatcgca
    21181 gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa
    21241 atcaacaatg tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt gatacactag
    21301 cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt
    21361 gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc
    21421 catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc
    21481 ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc
    21541 aggacaccat cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa
    21601 ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc aaggtattac
    21661 gtgccaataa gtatgacctt atcgtcaatc tcaccgatca atggatggtt gctatactgg
    21721 ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt
    21781 tttggcgtaa aagtttcacc catttggtgc cgttgcaggg tggaaatgtg gtggaaagta
    21841 acttatccgt gctgacccca ttgggagttg attcgttggt gaagcagaca accatgagtt
    21901 acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa
    21961 attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac aacgccaagt
    22021 tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc
    22081 cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag acgccaccag
    22141 taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc
    22201 agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc
    22261 tgatatcgct gtttggtgcg acagatcata ttttctggcg tccctggtca aataacatga
    22321 ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg
    22381 agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat aaattactgc
    22441 cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc
    22501 ctttggcggt ttgcagcgtg attttatgcg tattgctcag acagtcgccg cccgaggtca
    22561 tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat ttgaactgat
    22621 caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt ttgcctgggt
    22681 gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca aaatgccggg
    22741 gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtagcgc aggaaaaagg
    22801 ctttttctat cgcctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt
    22861 cgaacagggt aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca
    22921 gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga tttatcctga
    22981 tcgtaaatat agccagcagc cagccaatag ccgtgaaatc ttccgtaaga agaatggaat
    23041 aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga
    23101 tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattgctata
    23161 tgttgttggg caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgtg
    23221 cagtaatgtt cactttttct cggggcgcaa tgatgtctcg gaattaatgg cggcggcgga
    23281 tttattactg catcctgcct accaggaagc ggcgggaatt gtgctgctgg aagcgattac
    23341 tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgccttata ttgtcgaggc
    23401 taattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg
    23461 caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gatattatgc
    23521 tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa cgggtggtct
    23581 ggatggttga acttaaagag ccgtttgcca cgttatggcg cggcaaagat ccttttgagg
    23641 aagttaaaac cttgcagggt gaggtatttc gtgaactgga aactcgccgt actctgcgct
    23701 ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga
    23761 taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg
    23821 cgattcatcg actgcgggat gtcggcgttg atactatgca tggggtggca tttggcgaaa
    23881 aaggcatgaa tccgctgacc agaacttcat ttattattac cgaagatctg acaccaacca
    23941 taagtctgga agattactgt gctgactggg cgactaaccc tccagatgtt cgcgtaaagc
    24001 gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc
    24061 accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg
    24121 agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc
    24181 gttggcggga taaagatctt attgggcttt atttttcttc gatgaatatc ggcctgactc
    24241 agcgggatat ctggcggttt atgaaagtgt attttgccgc cccgcttaaa gacattctca
    24301 agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa
    24361 cgattcgaaa atcgttgtaa cccgtaactt atttttgcca aaattttgga tacagaataa
    24421 atatgtctca actcaatgat agtgacatca tcctttttga gtataatttt cattatcaaa
    24481 atataagatc taaaaatact cttgatatag catttggtat tgac
//
LOCUS       DAZFDP010000063        24458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFDP010000063 DAZFDP010000000
VERSION     DAZFDP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..767
                     /locus_tag="ACHJ1G_002582"
     CDS             <1..767
                     /locus_tag="ACHJ1G_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764422.1"
                     /translation="IQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGD
                     GYCLLRAILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVK
                     TLFGVNLQSDNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLV
                     LDTDPEQKVNEVKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDDLEYGYLINTG
                     NHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            1059..2300
                     /gene="agp"
                     /locus_tag="ACHJ1G_002583"
     CDS             1059..2300
                     /gene="agp"
                     /locus_tag="ACHJ1G_002583"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4764423.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2338..2565)
                     /gene="yccJ"
                     /locus_tag="ACHJ1G_002584"
     CDS             complement(2338..2565)
                     /gene="yccJ"
                     /locus_tag="ACHJ1G_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4764424.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2586..3182)
                     /gene="wrbA"
                     /locus_tag="ACHJ1G_002585"
     CDS             complement(2586..3182)
                     /gene="wrbA"
                     /locus_tag="ACHJ1G_002585"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4764425.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3555..3728
                     /gene="ymdF"
                     /locus_tag="ACHJ1G_002586"
     CDS             3555..3728
                     /gene="ymdF"
                     /locus_tag="ACHJ1G_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4764426.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(3811..5139)
                     /gene="rutG"
                     /locus_tag="ACHJ1G_002587"
     CDS             complement(3811..5139)
                     /gene="rutG"
                     /locus_tag="ACHJ1G_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4764427.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(5160..5654)
                     /gene="rutF"
                     /locus_tag="ACHJ1G_002588"
     CDS             complement(5160..5654)
                     /gene="rutF"
                     /locus_tag="ACHJ1G_002588"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4764428.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(5665..6255)
                     /gene="rutE"
                     /locus_tag="ACHJ1G_002589"
     CDS             complement(5665..6255)
                     /gene="rutE"
                     /locus_tag="ACHJ1G_002589"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4764429.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(6265..7065)
                     /gene="rutD"
                     /locus_tag="ACHJ1G_002590"
     CDS             complement(6265..7065)
                     /gene="rutD"
                     /locus_tag="ACHJ1G_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4764430.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(7073..7459)
                     /gene="rutC"
                     /locus_tag="ACHJ1G_002591"
     CDS             complement(7073..7459)
                     /gene="rutC"
                     /locus_tag="ACHJ1G_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4764431.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(7471..8163)
                     /gene="rutB"
                     /locus_tag="ACHJ1G_002592"
     CDS             complement(7471..8163)
                     /gene="rutB"
                     /locus_tag="ACHJ1G_002592"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4764432.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(8163..9254)
                     /gene="rutA"
                     /locus_tag="ACHJ1G_002593"
     CDS             complement(8163..9254)
                     /gene="rutA"
                     /locus_tag="ACHJ1G_002593"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4764433.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            9542..10180
                     /gene="rutR"
                     /locus_tag="ACHJ1G_002594"
     CDS             9542..10180
                     /gene="rutR"
                     /locus_tag="ACHJ1G_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4764434.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(10220..14182)
                     /gene="putA"
                     /locus_tag="ACHJ1G_002595"
     CDS             complement(10220..14182)
                     /gene="putA"
                     /locus_tag="ACHJ1G_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4764435.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            14237..14446
                     /locus_tag="ACHJ1G_002596"
     CDS             14237..14446
                     /locus_tag="ACHJ1G_002596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764436.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            14605..16113
                     /gene="putP"
                     /locus_tag="ACHJ1G_002597"
     CDS             14605..16113
                     /gene="putP"
                     /locus_tag="ACHJ1G_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4764437.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            16779..17609
                     /gene="efeU"
                     /locus_tag="ACHJ1G_002598"
     CDS             16779..17609
                     /gene="efeU"
                     /locus_tag="ACHJ1G_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4764438.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            17667..18794
                     /gene="efeO"
                     /locus_tag="ACHJ1G_002599"
     CDS             17667..18794
                     /gene="efeO"
                     /locus_tag="ACHJ1G_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4764439.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            18800..20071
                     /gene="efeB"
                     /locus_tag="ACHJ1G_002600"
     CDS             18800..20071
                     /gene="efeB"
                     /locus_tag="ACHJ1G_002600"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4764440.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            20690..21478
                     /gene="phoH"
                     /locus_tag="ACHJ1G_002601"
     CDS             20690..21478
                     /gene="phoH"
                     /locus_tag="ACHJ1G_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4764441.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            22059..22745
                     /locus_tag="ACHJ1G_002602"
     CDS             22059..22745
                     /locus_tag="ACHJ1G_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO4764442.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(22836..22972)
                     /locus_tag="ACHJ1G_002603"
     ncRNA           complement(22836..22972)
                     /ncRNA_class="other"
                     /locus_tag="ACHJ1G_002603"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            23191..24393
                     /locus_tag="ACHJ1G_002604"
     CDS             23191..24393
                     /locus_tag="ACHJ1G_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764443.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5924 a         6122 c         6513 g         5899 t
ORIGIN      
        1 gtatccagcc agaatggata aatacgccgg gaatatctga tttagatact ggtattgatt
       61 tgtctggtat atcttttatt cagagtgaaa ttaataaaac ttatggatta aaatacgctc
      121 cagtagatgg cgatggctat tgtctgttaa gggccatact ggttttaaaa gaacatgagt
      181 attcatgggc gctgggcagc cataagacgc aaaagcaagt atacgaagag tttattaaaa
      241 ttgttgataa acaaacgatc gaggctcttg ttgatacggc attcaatgat ctcagggaag
      301 atgtaaagac attattcggc gttaatctac aatctgacaa caaaattcag gggcagggtg
      361 gttttttatc atggagtttt ttgtctttta aaaaagaatt tattgatagt tgcttgaatg
      421 acaaaaaatg catactgcat ttacccgagt ttatatttaa tgataataag gcacgccttg
      481 ttttagacac cgacccagag cagaaggtta atgaggtgaa aaatttcctc actgctcttt
      541 cagatagcat ttgctcatta tttattgtta atggtaatgt ggcatcaatc tccttaggga
      601 atgaatcctt ttcaacagat gacgaccttg agtatgggta tttaataaac actggcaatc
      661 attatgacgt ttacctccct cctgaacttt ttgctcaggc ttacgagtta aacaataagg
      721 aaaggaatgc gcaaattgat tttttaactc gttatgcaat ttactgacaa aggcatgtgc
      781 taaaaaccat tgttattagc ctcacttctc ttattattgg tactattgcc tctgtattag
      841 taacgccgct gatattctgt ttagccacag gtgtaatcat cagcggcgta cgcgaggcag
      901 ggagctaatc aggcatagtt tgcgtcaaac cttgcctgtt tttaaagatg tatatagaaa
      961 aacaggcatt caacaagcca ttttgcgaac ctgttcccgg aaaaatgtca tatttctgtc
     1021 acactcttta gtgattgata acaaaagagg tgccaggaat gaacaaaacg ctaatcgccg
     1081 caactgtggc agggatagtt ttactcgctt caaatgctca ggcacaaacc gtaccggaag
     1141 gctatcagct acagcaagtg ctcatgatga gccgccataa cttacgtgcg ccgctggcga
     1201 acaatggcag tgtgctggag cagtctacgc cgaataaatg gcccgaatgg gacgtccccg
     1261 gtgggcaact caccaccaaa ggtggcgtgc tcgaagtgta tatgggccat tacatgcgtg
     1321 aatggctggc acagcagggg atggtgaaat cgggggaatg cccgccgccg gacaccgttt
     1381 atgcctatgc caatagtctg caacgtaccg ttgcgaccgc acagttcttt attaccggcg
     1441 cattcccggg gtgtgatatt cctgtgcatc accaggaaaa aatgggcact atggacccaa
     1501 cctttaaccc ggtgatcacc gatgattccg ccgcattcag tgaacaggcg gtggcggcaa
     1561 tggagaaaga gctcagcaaa ctccagctta ccgacagcta ccaactactg gaaaaaatcg
     1621 ttaactataa agattcccct gcctgtaaag agaaacaaca gtgttcgctg gtggatggca
     1681 aaaatacctt tagcgccaag tatcaacaag aaccaggtgt ttccgggccg ctgaaagtcg
     1741 gcaactcgct ggtagatgcg tttactttgc aatattacga aggttttccg atggatcagg
     1801 tggcctgggg agaaatcaaa tctgaccagc agtggaaggt gttgtcgaag cttaaaaacg
     1861 gctaccagga cagcctgttt acctcaccgg aagtggcgcg caatgttgcg aaaccgctgg
     1921 tcagttatat cgacaaagct ctggtcaccg atcgcaccag cgcaccgaaa attacagtgt
     1981 tggttgggca cgactccaac attgcctctc tgttaacggc gctggatttc aaaccgtatc
     2041 agttgcatga ccagaacgaa cgcacgccga ttggcggcaa aatcgttttc cagcgttggc
     2101 atgacagcaa agccaatcgc gatttgatga aaattgaata tgtgtatcag agtgcggaac
     2161 agttacgtaa tgccgatgcg ttaaccctgc aggcacctgc tcagcgtgtg acgctggaat
     2221 taagcggttg cccgatagat gccaacggtt tctgcccaat ggataagttt gatagcgtgt
     2281 tgaatgaggc ggtgaaataa cagaaaactc ccccgcgaga agtgggggag tcgctggtta
     2341 aacgttttta cgttcgatgg tctgttcgcc ccaaaaaagc gaatctttat cggtcttagc
     2401 aaaggctttg actaacactt catcgctacc ttcttcccaa atcttttccg ccattttttc
     2461 gtcgtacccg gcgacttcga aaatggcctc ggctatttcc ggcgacgtat tgcgcagaga
     2521 tgcccattca ccgacgtgat gagctttcgc ttcttgagtt ggcatgcgta tcctcctgtt
     2581 gaagattagc cgttaagttt aactgccaga cctgcgacat attccccttg ataacgagca
     2641 atagacagtt cttcctggct tggctggcgt gagccgtcac cgcctgcgat ggtggttgcg
     2701 ccgtacggcg taccgccgcg aacctgtgaa acgtcaaata attcctgcgc tgcgtagcca
     2761 atggggacaa ttaccatgcc gtgatgcgca agggtcgtcc aggttgaagt aatagtttgt
     2821 tcctgaccgc cgccagtacc ggtggaacta aagacgctcg ccagttttcc gtatagtgcg
     2881 ccggaagccc acaggccgcc cgtctggtcg aggaaggtac gcatttgacc ggacatgttg
     2941 ccaaagcggg taggtgtacc aaaaataatg gcgtcgtaat cggccagttc ttgcggggtt
     3001 gcaaccggtg cagtttgcgt tttaccgcct gctttttcaa ataattgcgg cggcatggtt
     3061 tccggtacac gcttaacgac aacttctgcg ccatcgactt tgcttgcacc ctcagcgact
     3121 gcgcgtgcca tcgtttcaat atgtccgtac atggaataat aaagcaccag aactttagcc
     3181 atttctaacc actcctcgtg ttatctctat tccgtagcga ttcgctacca cttatttaaa
     3241 gataagacgt ccttttcaga gtgcaaattt cacaaccact tatttgattt ataacaactt
     3301 tcacaagcac gtaattttgt cgcaaaatga cacattttta tctcatcgcg tttttttaat
     3361 cataagagcg gcttatggat aattatttga gatgatatct attctcgcta agaagctgtt
     3421 gcaggatatt accaaacgcg ggtctgcccg cgtcagttca ctaagcttag tcccacgtag
     3481 cgaaaatatg gcagccgcca tatgccgcgt taattctatg caatatgatg tctataccca
     3541 gacggaggtc agtaatggca aaccatcgag gcggttccgg caattttgca gaagaccgcg
     3601 aaagagcatc agaagcaggt aaaaaaggtg gacagcacag cgggggtaat ttcaagaatg
     3661 acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa gagcagtcac ggcaaaagcg
     3721 acaactagcc gggctaatca atgacgaatg catttttttc ttaggctcgt caaaaagtca
     3781 tcaccgccgg taatcccggc ggttttctgt ttatggctcc tgatgaacaa cttctggcgg
     3841 tggaacgtca accaactttc tgcttaacaa cgcattgagt aaaatcgcgc caaaggttgc
     3901 tgtaccaatc cctcccaacg taaaaccgcc cagcgtgaga gcaaaatcac ccgcgcccag
     3961 cactaaggtt actgcgacca taatcaaatt accgttctgg cttaaatcga cacggttttg
     4021 tacccatatc cttgcgcctg cgacggcaat cagcccgaac acaacaattg atgcaccacc
     4081 aatcaccgcg gccggaatgg tatgaatcag cgcgccaaat ttcggtgaaa agcccaacag
     4141 catggcgatg acggcagcag cgacaaacac cagcgtcgag tagacttttg tcacggccat
     4201 tacaccgata ttctcggcgt aggtggttac gccgctgcca ccgacagaac cagaaagcat
     4261 cgtcgccagc ccatcaccga cgaatgcccg ccccatatac gggtccatat tgcgtccggt
     4321 catcccggcg accgccttga gatgacctaa gttttccgcc accagaatca ccgccacggg
     4381 cgcaatcagc atcattgcct gactattaaa agcaggagtg gaaaaatgtg gcaaaccgaa
     4441 ccaggcagca tggctgacga gagtaaaatc gacggctttt cccagcccta aaaggttggt
     4501 catcacgcca tacagcagac aggcgacaat taaacctacg agaatcaata accgctggat
     4561 catgccacgg gtaaacaccg ccaccagccc aatacacagc accgtcatta ccgccatcca
     4621 gctatcaaag gccgaagccg atacactttt cactgcgata ggcgctaagt tcaggccaat
     4681 cgccatcacc accgcacccg tcaccaccgg cggcattagt cgttcaatcc agcgtgtacc
     4741 aattttcatc accaccaggc caatgacggt ataaaccagc ccacaggcga taatcccgcc
     4801 cagcgcaatg ctgatattcg ggttaatgcc ctgaccgtta aagcccgtcg cggcaatcac
     4861 cacgccgaca aaagccgcgc tggaaccgag ataactgggg acgcgcccgc cggtaataaa
     4921 gaaaaacagt aacgtgccga tccccgacat taaaatggaa agattgggat ccagccccat
     4981 cagaatcggc attaacaccg tcgcgccaaa catcgccacc gcgtgttgaa cgcccattac
     5041 tgccgtctga gcaaacggca atcgttcata cggcgcgacc acgccgctct ctgtagaggt
     5101 cgattttaac tgccagtgag gaaaaccgaa cattgccatc agctgtctcc tttaggaggt
     5161 taacaagcag ggcgcatcag cgcgtgataa ctgcgatcga accacaccag cccgtagggt
     5221 gtggcgtgac gatgaatcgc ttcgatggcg caaaacagaa tgtcgtgggt gccgacgctc
     5281 accacctggc tgatacggca gtcaaacgaa accagagcct cttccagttg cgggcatccg
     5341 gtcacacccg tctgccagcg ggcggcggca aagcggtgtt ccatgggcgt tttgccgcca
     5401 aaaagatttg aaagcggctc ctgcccggcg ctcagtgtat ttacacacag cgttcgattt
     5461 tcattgaata ccggccagac ggacgcccca cgattcaggc acaccagtaa tgtaggcggc
     5521 gtgtcggtca cgctgcagac cgcgctggcg gtgaacccgg cgcgcccggc tggaccatcc
     5581 gtggtgatga tattgactgc cgcgcccata caggacatcg catcgcgaaa ggtttgttga
     5641 tcgacaatgt tcatggtttg ctccttacaa cagcccgcag gcttcttcaa aggacagacg
     5701 tggcaggcgc gcaaaaagtt tgctgctatc gccatagccg atattaatca gcagattgct
     5761 cttcagcgtg ctgcccgcaa aaaaggcgtc gtcaacgtgt tgacggtcaa aacccgacat
     5821 cgggccggta tccagtccca gcgcacggca ggcgacgatc agataggccg cctgcatgga
     5881 actgttgcga aacgctgttt cttcggcaag ttgtgggctg gaggtaaacc aactgcgggc
     5941 atcgccgtga ggaaacagtt gcggtaaccg ttcataaaat tcactgtccc aggcgacgat
     6001 agcggtgacg ggcgcggtca gggttttttg cagattgccg ctggaaagtg ccgggcgcag
     6061 acgttctttt ccttctgccg tgcggataaa cacgatccgt gccggagaac agttagctga
     6121 tgtcggcccc catttcatca gggcataaat ctcccgtaac gtctcatcgc tgacgggtgt
     6181 ctcccgccag ccgttgtgag tgcgggcatc ggtgaacaag gtgctaagcg cacctgggct
     6241 aacggcttcg ttcatagcaa ttccttacag ggcggcttca cggtgatgta acaggctggc
     6301 aagcccgttg agtaacagag cattaaacgt ttcgggatcg gtcacgttgc aggcgtgtcc
     6361 gccatagggc atcaccattt tctggctatc gggcagggcg gcatgaagct cactggaaca
     6421 tgctgatggc accagcagat catcactggc gcagatgatt tgcaccgggc agcggatgcg
     6481 atccgcatgg tgactaaagt cagcgcgttt gagggcgtta agtcgacgca gtaaattatt
     6541 tttgccctga aaatgcgcca gtgccagcgc gtcttctgcc tccaggcgag gtgcgcgggc
     6601 cgccatccag tcggcgggat agaggaacaa cggctgcgct tccacccatg cctgcgcgcc
     6661 gccgctatac agtaatcgtt cgcgaacctg aaaacagcgg cgcgtatggg cgtttattcg
     6721 tagccagccg ttaacgctga tcagcacagt taccgacgcg ggataatcca gcgccagctg
     6781 cattcccacc agcgcaccga gcgcatggcc gaccaccgcg taatgctcaa tccctgcggc
     6841 taccagcgcc tgatgcagtt ccgctgccat ctgggcgata ctgtaatctt ctgccagcgt
     6901 gtcgggatta ttgccggtgc cgcgctggtc gtaacagact acctgatact cctgctccag
     6961 caccgccagt tgcggtaacc agtaactgcc gctaccgcca agacccgaaa tcaacaccac
     7021 tacgggcgta tcagcataag ggggaggtga gagtgaaagt ttcatcgcgg cctcacttgg
     7081 cgatatgcgc aattgtggcg atttccacca gcgcgtcagg ttttaccagt ccgcactgaa
     7141 tgcagaatcg cgccggttta tcacccggaa aaaactcggc gtagatttcg ttaatcgcgg
     7201 cgtaattttt ccagtcggta ataaagatgc tgttgaaggt cacatccgcc atcgtgccac
     7261 ccgccgtctc gatcaccttg cggataattt ccagaacgtg gcgggtttgc gcctttgggt
     7321 catcggcaaa cagcacgtta ttatgttgat caaaagccag cgtaccggag acatacacca
     7381 cgccatcagc cagcgtgccg ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa
     7441 taattacgga ttttggcatc gttaaactcc ttaagcgata cgagcaaagg atgtgggaga
     7501 aagcgcgtcg cagaacgttt cgacgtcgct gacccagcca aaaaaggttt cgatattgaa
     7561 caacgcggct ttctgtgcaa attccggccc cgcctggtgt gttgcgtctt ccagcaccac
     7621 gccgaaatac tccagaaaaa agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc
     7681 gataccggtg aaaaccagat ggcgtattcc acggctgcgc aaaatgctgt ccagcggtgt
     7741 attgaagaaa ccgctgtagc gcggcttcgg cagcacaata tcgccaggct gcggcaccag
     7801 ttcatccacc agttgataat cccaggagcc tttcgccagc aatttcccct gcagctgcgg
     7861 ctgcttacgc atggttttca gggcgttcga tttatgaaaa ttcggtgagc cggggccgcc
     7921 agcttcgaca tactgttcat cccagccatt ttgaaaccag atgatcagca tccctgccgc
     7981 tcgcgctgcg gtcacggcgg tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc
     8041 aaacccggcg agatctaagt agccgcctgg cgtggcataa gcgttttgca tatccaccac
     8101 gatcagcgca ctttgctgcg gatcgaaggt aatggcttcc ggtcgagcgg ttaaggttgt
     8161 catcatgcca cctcctgagt cagcgcaggt agatgggcgc ggcactgcat cagtggttga
     8221 atgcgctcgc cgaaggtttc gattcccgac agaaaatcat cgaaggtcaa cagcacgcct
     8281 tcggcaccag gcacgcttgc gacttcatct aacatgcgcg cgacactggc gtaagaaccg
     8341 actaacgtcc ccatattgat gtttaccgcc gaagtgggat cggccatctg acggacgttg
     8401 gtatcagtac cggagcgggt atctttctga ctttgttcgg ttagccagct taacgcctct
     8461 tcatccgcgc ccgctttgta gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg
     8521 gcaatcacca taaacaacac ataagaacca acgtcgcgcc cggtttgctc tgcggcctgt
     8581 ttcatccgcg cagcggtcgg ggcgaaagcc gtgggcgtat ttacgccttt gccgaaacag
     8641 aagttgaaat cggcatactg ggcggagaac gccatgccag cgtcgctttg cccggcgcag
     8701 atcactttca tggggacact cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa
     8761 tcgcctttaa aatcgctttt ccccgtgccc cacaggtcgc gcagcacctg aacatattcg
     8821 gtgagatagt cgtaacgacg ggagaaatag tcatcgccag gccagatacc catctgctca
     8881 tactcgggct tttgccagcc agtcacgagg ttgacgccaa aacgcccgcc agagatggag
     8941 tcgatggttg cggccatacg ggcgacgatt gccggaggta acgttaaggt ggcagcagtg
     9001 gcgtaaatct gaatgcgcga ggtgacggcc gccagccccg ccatcaaggt gaacgactca
     9061 aggttgtgat cccagaactc agttttgccg ccaaagccac gcagtttgat catcgacagg
     9121 gcgaaatcga aatggtagtg ctccgctttt tgcacaatgg ctttattcag ttcaaaggtc
     9181 ggcatgtact gcggcgcgtg ggtcgaaatg agccagccgt tgttgccaat aggtacgaat
     9241 acgccaattt tcatcatcaa cctctcttcg tctcgtaaag tgaaagtcag acggggcgct
     9301 gcatcctgca tatccttttc agccgcgtaa tggcttgttt gcaaagcgga tgccagtttt
     9361 taaaaagtga atgttattaa tgtgttaaca ttgtgttgtc taaaatatct ggtaaaaagt
     9421 ggactaaacg gtcaaaacag ttgcacataa aacatgcatc tgtgcgcgat gagagtgcag
     9481 aaggtcgagg ccgggcgggg gttttgctat cctgttgcca atctacaaga ggggagagcg
     9541 catgacgcaa ggcgcagtga aaacaacggg taaacgttcg cgcgcagtaa gcgcgaagaa
     9601 aaaagcgatt cttagcgcag cactggacac tttttcacaa ttcggttttc acggcacaag
     9661 gctggagcag atcgcagagt tggcgggtgt ttcaaaaacc aatctgctgt attactttcc
     9721 gtcaaaagag gcgctgtata ttgccgtgct gcggcagatt ctcgatatct ggctggcacc
     9781 gttaaaagcg tttcgtgaag atttcgctcc gctggcggcg ataaaagagt acatccgtct
     9841 gaagctggaa gtctcccgcg attatccgca ggcttcgcgc ctgttctgta tggagatgct
     9901 ggcaggcgcg ccgctgttaa tggatgaact gacgggcgat ttgaaggcat taattgatga
     9961 gaaatcggcg ctgattgccg gttgggtcaa aagcggcaaa ctcgcgccga ttgatccgca
    10021 gcatttgatt ttcatgattt gggcttccac tcaacattac gccgatttcg cccctcaggt
    10081 ggaggcggtg acaggcgcga cgttgcgcga tgagatattt ttcaatcaaa cggttgaaaa
    10141 cgtgcagcgg attattattg aggggattcg accacgttaa agatgccgga ggaggttgta
    10201 acatcctccg gctacctgtt taacctatag tcattaaact ggcgttaccg ccagcggcag
    10261 cggtattcac actcagcgaa cgttcgatat acagccgttc cagaaggata ttgctttcgc
    10321 cacgggcaaa accctgcacc gaaacaattg cgccatcccg cgcagcaacg gcttcacaca
    10381 atgcgcgaag ctgatccgaa tcaccgtgga agatcaccgc atcaaagggt tgagcggtta
    10441 tattttccgc tttcgccagt tgaatacgtt cgctgactgc cgatggcaat gcctttacta
    10501 gctgacgatg cagcgcgtca tccggccaca gtacctggct gcccaccgcc agcacggcgg
    10561 cgagctgagt cagcgcatcc tgctcatcat cggcaataca caacacgcgc tcacgcggca
    10621 gcagcgtcca ggtgttgcgt tcacccgtcg gccccggcag caatcgttgt gttcctgcct
    10681 gcgccagctc gccatattgc gtacataacg cctgcaattc tggacgattt gctgcccatt
    10741 cccgcagtgc atttagcggc tgagtcaatg cggctttcaa ctgtgcatcg accggatact
    10801 ctgcatcctg acgcgcgagc gtcactgcca gcgcactttc cgggcgattc gccagcagac
    10861 ggtagagata gagcggaccg cctgctttcg gcccggtacc ggacaaccct tcgccgccga
    10921 atggctgcac acccaccact gcgcccacca tattacggtt aacatacagg ttaccaacat
    10981 gggccgagcc agtgacctgg gcgatggttt catcaatacg cgtatggaca ccaagcgtca
    11041 gaccatagcc ggaagcgtta atctgctcga tcagctctgg tagctggtta cggttgtaac
    11101 gcaccacatg cagcaccgga ccaaagacct ctttttgcaa ttcggcaaag tcatccagtt
    11161 cgatcagcgt cggggcgaca aaggtgccgc tttgccattc acgggcatct tcgctgtttt
    11221 cccgcaccgc ctggaacacc ggacggcctt tgctacgcat ggtctgaata tggcgctcaa
    11281 tattggcttt cgcttcgcta tcaatcaccg gaccgatatc ggtggtcagg cgacccggat
    11341 tacccatccg gcattcggcc attgcgccgc gcagcatttt caacgtgtgg tcggcaatct
    11401 catcttgcag gcacagcacg cgcagcgccg aacaacgctg acccgcactg tcgaacgccg
    11461 aggccagtac atccacgacg acctgttcgg tcagtgctga agaatcgaca atcatcgcgt
    11521 tcatgccgcc ggtttcagcg atgagcggaa tagggcgacc ctgagcgtcc aggcggctgg
    11581 cgatattgcg ctgcagtaac gtagcgactt cggttgaacc ggtaaacatc accccgcgta
    11641 cgcgatcatc acccgtcagt tgcgcgccca cggtttcccc ctgacctggc agcaactgca
    11701 ccacgcctgg cggtacaccc gcttccagca aaatggcgat cccttgcgcg gcaatcagcg
    11761 gcgtttgttc tgccggtttt gccagcacgc tgttacctgc cgccagtgcg gcagcaatct
    11821 gcccggtgaa aatagccagc gggaagttcc acggactgat acacaccaca ggccctaatg
    11881 gacggtgggt ttcgttagcg aaatcatccc gcacctgtcc ggcatagtag tggagaaaat
    11941 cgaccgcttc gcgcacttcg gcaatggcgt tactgaaggt ttttccggct tcacgcacca
    12001 gaataccaat cagttgctgc atctggcttt ccattagcac ggcagcgcga tgcaaaatcg
    12061 ccgcgcgttc aaccggagga gtggcaaacc agattggcgc gttattaacc gcactttcca
    12121 gcgcctgttc tacctcacgc ggcgtggctt cacgcacaaa gcccacaata tctttcggtt
    12181 ccgcagggtt aataacgggc gacatctcac ctgccgctac cggttgttcc agcattggca
    12241 aggcctgcca tttttgcagt gcactattga gcagggcaga agagagcgag gccagccggt
    12301 gttcgttagc gagatccagc cctgccgagt tgtcgcgccc gtgaccgtaa agatcgcgcg
    12361 gcaggggaat tttcggatgc ggtaatccag tttgcccttc ctgttgcgcc agtttttcta
    12421 cagcagtgac cggatcggcg accagttcat ccagtggcaa agaggtgtcg gcaatacggt
    12481 taacaaacga ggtgttagca ccgttttcca gcaggcgacg caccagatac gccaacagcg
    12541 tttcatgggt accgaccgga gcataaatac gacacggacg gttaagtttg ccgtcggcaa
    12601 ctttcccggt gacctgctca tacagtggct cgcccatacc atgcaggcac tggaactcgt
    12661 actgacccgg atagtagttc tgacctgcca gttgataaat cgctgccagc gtatgggcgt
    12721 tgtgcgtcgc gaactgcggg tagattagat tcggcaccgc cagcagcttt ttcgcacagg
    12781 cgagatatga aacgtcggta tacaccttgc gggtataaac cggatagcct tcaaggccgt
    12841 ccatctgcgc acgcttgatt tcactatccc agtacgcgcc tttcaccagg cgaatcatca
    12901 gacggcgacg gctgcgggtg gcgagatcaa tcaggtaatc aatcaccagt gggcagcgtt
    12961 tttgataagc ctgaataaca aaaccgatac cgttccagcc tgccagctca ggctcgaaac
    13021 agagtttttc cagcagatcg agggagatct ccagacgatc ggcctcttcg gcgtcaatgt
    13081 tgataccaat atcgtactga cgcgccagca gggtgagtga tttcagacgt gggtaaagct
    13141 cttccattac ccggtcatat tgggcgcggc tgtaacgcgg atgcagcgcc gacagcttga
    13201 ttgaaatgcc cggaccttca tagatgccac gaccattgga cgctttacca atggcgtgaa
    13261 tcgcctgctg ataggaaacc atatacgctt gtgcatctgc ggcagtcagt gcagcttcgc
    13321 caagcatatc gtaagagtaa cggaaacctt tctcttccag cttgcgggca ttggctaacg
    13381 cttccgcgat ggtttcgcca gtgacgaact gctcgcccat caggcgcatc gccatatcca
    13441 cacctttgcg gatcagcggt tcaccgcttt taccgataat gcggttcagc gagcgggaga
    13501 ggctggcttc gttatgggtg gaaaccagtt tgccagtaaa cagcagcccc caggtggcgg
    13561 cattaacaaa caaagacggg ctacgaccaa tgtgtgattg ccagttaccg ttgctgattt
    13621 tgtcgcgaat taacgcatca cgggtggctt tgtcgggaat acgcaacaac gcttccgcca
    13681 gacacatcag cgccacgcct tcctgcgatg acaacgaaaa ctcctgcaat aacccctgaa
    13741 ccatacctgc gcgaccactg gcgttttttt gattacgcag tttatcggcc agttgatacg
    13801 ccagtttgtg cgcctgttca gcaactggct gcggcaggcg ggcttgttcc agcagcatag
    13861 aaaccgcttc ggtttccggg cggcgatagg ccgctgtgat cgcggcgcgg gaaaccgaca
    13921 ggggcaaaat ttgctcggca aagtcgagga atggttggtg tggttcctct gccggagtcg
    13981 gtgcttcatc gctctcattg gcagcgccag aaagcagcgc aggtagctcc ggcagagtat
    14041 cgctgttttc cagttgttcg agataagaaa aaatcgcctg cttaattaac cagtgtggcg
    14101 tgcgatcgat acgtgtcgcg gcagacttaa tacgctcacg cgtcgcgtcg tccagcttaa
    14161 cccccatggt ggtggttccc atgccattac tcctgttgtt ctgaaaggtg caacttaacg
    14221 ttatcgtgaa atatccatga tgttgcaact ttgtgcaacc atgttaaatg tgacatgcgt
    14281 agcaagctta aaaatgaatg aaatgttaat aaaagaaatc gatatgacag ggattaaaaa
    14341 aataactcag actttttctc tgcggcagtt aacatttttg aaaggtgcaa ccgcaaaaaa
    14401 tgtgagagag tgcaacctga tgaaaaatag tgtcgctgag cactaaaatt taatgtaaat
    14461 ggtgtgttaa atcgattgtg aataaccagc gcttccggca ggatacggtc gccctggtaa
    14521 aacataaact ctgttacccc gttccggtgg cagatataac ggcaagtttc gacattgccg
    14581 ataataattt tttggagact ttagatggct attagcacac cgatgttggt gacattttgt
    14641 gtctatatct ttggcatgat attgattggg tttatcgcct ggcgatcaac gaaaaacttt
    14701 gacgactata ttctgggcgg tcgtagcctt gggccattcg tgacggcatt atcggcgggt
    14761 gcgtcggata tgagcggctg gctgttaatg gggttgccgg gcgcggtttt tctttccggg
    14821 atttccgaaa gttggatcgc cattggcctg acattaggcg cgtggatcaa ctggaagctg
    14881 gtggcggggc ggttgcgtgt gcataccgaa tacaacaata acgccttaac attgccggat
    14941 tatttcaccg ggcgctttga agataaaagc cgcattttgc gcattatctc cgcgctggtg
    15001 attttgctgt tcttcaccat ttattgcgct tcgggcattg tggcaggcgc gcgtctgttt
    15061 gaaagtacct ttggcatgag ctacgaaacg gctctgtggg cgggagctgc ggcgacgatc
    15121 ctttacacct ttattggcgg tttcctcgcg gtgagctgga ctgacactgt acaggccagc
    15181 ctgatgattt ttgccctgat cctgacgccg gttatcgtca ttatcagtgt cggtggcttt
    15241 ggtgactcgc tggaagtgat caaacaaaag agcatcgaaa acgttgatat gctcaaaggg
    15301 ctgaactttg tcgccattat ctcactgatg ggttgggggc tgggttactt cgggcagccg
    15361 cacatcctgg cgcgttttat ggcggcagac tctcaccaca gcattgtcca tgcgcgtcgt
    15421 atcagtatga cctggatgat cctctgcctg gcaggggcgg tggctgttgg cttctttggg
    15481 attgcttact ttaatgagca cccggcggta gcaggcgcgg taaaccagaa cgccgagcgc
    15541 gtgtttatcg aactggcgca aattctgttt aacccgtgga ttgccgggat tctgctgtcg
    15601 gcgattctgg cggcggtaat gtcaacctta agttgccagc tgctggtatg ttccagtgca
    15661 attaccgaag atttatacaa agcgtttctg cgtaaacagg ccagccagaa agagctggtg
    15721 tgggtaggac gtgtgatggt gctggtggtg gcgctggtgg cgattgcgct ggcggcaaac
    15781 ccggaaaacc gcgtgctggg cttagtgagc tacgcgtggg caggctttgg cgcggcgttt
    15841 ggtccggtgg tgctgttctc ggtgatgtgg tcacgcatga cgcgcaacgg tgcgctggcg
    15901 gggatgatca tcggtgcgct gacggttatc gtctggaagc agttcggctg gctgggactc
    15961 tacgaaatta ttccgggctt catcttcggc agtatcggga ttgtagtgtt tagtttgctg
    16021 ggtaaagcgc cgtcagcggc gatgcaaaaa cgctttgccg aggccgatgc gcactatcat
    16081 tcggctccgc cgtcacggtt gcaggaaagc taagggactt agcctgcggc ggttttgttt
    16141 ggcttcagca gcgggttgcg ctcccttaat gtgcctcgcc atataaattg aatggtgcag
    16201 ggagcgcgca gggggcggcc aatcgccgcg gccccctgca cccccgggct ctggcgaaca
    16261 aaatcgccgc tgcgtggtac cttcagctta tccccttcgg ctaccgggtc gggcgcgagg
    16321 taacgtcctg taagacgcgc cctcagccca catccatgtg ggctgccccg gccttcgggg
    16381 aacgtttcag cgattttgac gccaccaaca cccgcgctgt tcttatgttc cgggcaaaaa
    16441 gttagatttg ataatcgagg atggacgaaa ttgcttgata cacccgctta tcagttttac
    16501 atggaagttt tgatgtattg agtcaggacg gttttgtcgg ctggatacgg cgtttacgcg
    16561 gcatccggca agaacacatg gctcgttgta aacaatccca tctttgtacc tggaataatc
    16621 gtttatatcc cctggcatta cctctctttg tttacatttc aacatcattt tataaacatt
    16681 ccgcttgtat ttttctttac cgtaatgata atcgctatca ctgcgattta cttttctttg
    16741 catagattga ctcagaaaaa cgtttaagga tgggtggcat gtttgttccg tttctcatta
    16801 tgttgcgcga aggacttgaa gccgcgctga ttgtcagttt gattgccagc tatcttaagc
    16861 gtacccagcg aggccgatgg attggtgtga tgtggattgg cgtgttgctt gccgctgcgt
    16921 tgtgcctggg cttggggatc ttcattaacg aaaccaccgg cgaatttccg caaaaagagc
    16981 aggaactgtt tgaaggtatc gtggcggtga tcgccgtggt gatccttacc tggatggttt
    17041 tctggatgcg caaagtgtcg cgcaacgtca aagtacaact ggagcaggca gtcgatagcg
    17101 cattgcagcg tggaaatcat catggctggg cgctggtgat gatggtcttt tttgccgttg
    17161 caagggaagg gctggagtcg gtctttttcc tgctggcggc atttcaacaa gatgtcggga
    17221 tctggccgcc gctgggtgca atgctcggtc ttgctactgc cgtggtgcta ggcttcttgc
    17281 tctactgggg cggtattcgc ctcaatcttg gtgcattttt taaatggacc agcctgttta
    17341 ttctcttcgt cgccgcaggg ctggcagctg gtgccattcg cgcatttcat gaagccggat
    17401 tgtggaacca ctttcaggaa atcgccttcg atatgagtgc ggtgctctca actcactcgc
    17461 tgtttggcac gctgatggaa gggatttttg gctatcagga agcgccgagc gtcagcgaag
    17521 tcgccgtctg gtttatttat ctcatcccgg cgctggtggc atttgctctg ccaccacgcg
    17581 caggggcgac agcgtctcgc tccgcgtaac aaatacgacg caaactcttg cttagttaca
    17641 acatacttta aagggatagt ctcgtcatga ccattaactt ccgccgtaac gcattgcagt
    17701 tgagcgtggc tgcgctgttt tcttctgctt ttatggctaa cgccgctgat gtgacgcagg
    17761 tcaaagtgac cgtgacggat aagcagtgcg aaccgatgac cattacggtt aacgccggga
    17821 aaacacagtt cattattcag aaccatagcc agaaggcgct ggagtgggag atcctcaaag
    17881 gcgtgatggt ggtggaagag cgggaaaata tcgcccctgg ttttagccag aaaatgacgg
    17941 cgaatttaca gcctggcgaa tacgatatga cctgcggtct gctgactaac ccgaaaggga
    18001 agttgatcgt caaaggtgag gcaacggcgg atgcggcgca aagtgatgcg ctgttaagtc
    18061 ttggtggtgc aattactgca tataaagcgt atgtcatggc ggaaaccacg cagctggtga
    18121 ccgacaccaa agcctttacc gacgcgatta aagcaggcga tatcgaaaaa gcgaaagcac
    18181 tgtatgcgcc gacgcgccag cactatgagc gcattgaacc gattgctgaa ctgttctccg
    18241 atctggatgg cagcattgac gcccgtgaag atgattacga gcaaaaagcc gctgatccaa
    18301 aattcaccgg tttccaccgt ctggaaaaag cattgtttgg cgacaacacc accaaaggca
    18361 tggatcagta cgctgaccag ctttataccg atgtggtcga tttgcaaaaa cgcatcagtg
    18421 aactggcttt cccaccttca aaagtggtcg gcggtgcagc cggactgatt gaggaagtgg
    18481 cagccagcaa aatcagcggt gaagaagatc gctacagcca caccgatctg tgggatttcc
    18541 aggctaacgt tgaaggctcg cagaaaattg tcgatctgct gcgtccacaa ctgcaaaaag
    18601 ccaacccgga actgttggca aaagtcgatg ccaactttaa aaaggtcgat accattctgg
    18661 cgaaataccg tactaaagac ggttttgaaa cctacgacaa attgaccgat gccgaccgga
    18721 atgcactgaa aggaccgatt actgcgctgg cggaagatct ggcgcaactt cgcggtgtgc
    18781 tgggattgga ttaagcatta tgcagtatga agataaaaac ggcgtgaatg aaccgtcacg
    18841 ccgacgttta ctgaaaggca ttggtgcgct ggcgctggcg ggaagttgtc cggtcgctca
    18901 tgcacaaaaa acgcaaagtg cgccgggtac gctttcaccg gatgcacgca atgagaaaca
    18961 gccgttttat ggtgagcatc aggcagggat cctgacgcca caacaggccg caatgatgct
    19021 ggtggcgttt gatgtgcttg ccagcgataa agccgatctt gaacggttgt ttcgcttgtt
    19081 gactcagcgt tttgcttttc tgactcaggg cggagcggca ccagaaacgc caaatccgcg
    19141 cctgccacca ctcgattccg gcattcttgg cggctacatt gcgcccgata atctcaccat
    19201 cacgttatcg gtgggtcact cattgtttga tgagcgcttt ggccttgcgc cacagatgcc
    19261 gaaaaagttg cagaagatga cgcgttttcc caacgactcg ctggatgcgg cgttatgtca
    19321 tggtgatgtg ttgctacaaa tttgcgccaa cacccaggac acggtgatcc atgcactgcg
    19381 cgatatcatc aaacatacgc cggatttgct cagcgtgcgc tggaagcggg aagggtttat
    19441 ttccgatcac gcggcgcgta gtaaaggcaa agagacgccg attaatttgc tgggtttcaa
    19501 agacggcact gccaatcccg atagccagaa tgataagttg atgcaaaaag tggtgtgggt
    19561 aacggcagat cagcaggagc cagcgtggac aatcggtggc agctatcagg cagtacgctt
    19621 gattcagttt cgagtggaat tttgggacag aactccgctg aaagaacagc agacgatttt
    19681 tggtcgtgat aagcaaaccg gtgcgccgct gggaatgcag catgagcatg atgttcccga
    19741 ttacgccagc gatccggaag ggaaggtgat tgcgctggac agccatatcc ggctggcgaa
    19801 tccccgcacg gcggagagtg agtccagcct gatgctgcgt cgtggctaca gttattcact
    19861 gggcgtcacc aactccgggc aactggatat ggggctactg tttgtctgtt accaacacga
    19921 tctggaaaaa ggcttcctga cagtacaaaa aaggctcaat ggcgaagcgc tggaggaata
    19981 cgttaaacct atcggcggcg gttatttttt tgcgctgccg ggagtgaagg acgcgaacga
    20041 ttatctcgga agcgcgttat tgcgggttta atgtttatag acggataagg cgttcttgcg
    20101 cagatgcctg atgcgatgct tgcgcgtctt cttatgccta caatcggtgc gggttcggta
    20161 ggcggataag gcgttcacgc cgcatccggc gttcgtgcgc agatgcctga tgcgaatcct
    20221 gctgaaagca cacagctttt ttcatatctg tcatcactct gtcatctttc cagtagaaac
    20281 taatgtcact gaaatggtgt tttatggtta aatataagtg aatatattgt tgcaatgaat
    20341 gcgagatctg ttgtaattat taagtagcag cggaagttcc cggcagtgat agtcagtcac
    20401 tatggagatc gcggatggta acgtcctgtt ctggacaatg tcttgttaag ctacgctcca
    20461 ccacttgcgg agatttctcc tccggtagca acttcgtcac tctgtatttt tctcacttcc
    20521 tctctttact cgtgttaccg acgccgcaaa cggtgtgcgt agccgttttt cggcatttta
    20581 tccaaaagca aactatggct tacaaggaag ccaaccctct gatgttcgtg cgcataatcg
    20641 cgctgccaac ggcgcgtgtg atgaatacaa acaactcaag gtgctctcca tgggaagaca
    20701 aaaagcagtg atcaaagctc gtcgcgaggc aaaacgtgtg ctgagacggg attcgcgcag
    20761 ccataagcag cgtgaagaag aatcggtcac ctcgcttgtg cagatgggcg gcgtagaagc
    20821 cattggtatg gcccgcgaca gtcgcgatac ttcgcccatc ctcgcgcgaa atgaagcgca
    20881 attgcactat ctgaaggcta ttgagagtaa gcagctgata ttcgccacgg gcgaagccgg
    20941 gtgcggaaaa acctggatca gcgcagcaaa agcggcagaa gccctgatac ataaggatgt
    21001 cgacaggatt atcgtcaccc gtccagttct gcaagccgat gaagatcttg gcttcttacc
    21061 tggagatatc gcagaaaagt ttgctcccta ttttcgcccg gtctacgacg tgctggtccg
    21121 gcgcttaggg gcttccttta tgcagtactg cctgcgaccg gaaataggga aggtggaaat
    21181 tgcgccgttc gcctatatgc gtggacgtac ctttgaaaat gcagtcgtca ttcttgacga
    21241 ggcgcagaat gtgactgccg cgcaaatgaa aatgttttta acccgcctcg gggagaacgt
    21301 gacggttatc gtcaacggtg atatcacgca atgcgatttg cctcgcggcg tgtgctccgg
    21361 attaagtgac gcgctggaac gtttcgaaga agatgaaatg gttgggatcg tccggttcgg
    21421 taaagaggac tgcgtacgtt cggcactttg ccaacgtacg ctgcatgcct acagttaagt
    21481 gtattatcgg tgcagagccc gggcgaaccg ggctttgttt tatttcaaca gattttcact
    21541 tcaggttagc gttcaggggg gcgactttga gcgtgttgat aagctgaact ggagtctggt
    21601 ttagatactc ttaccaggca tttttcgtca tagcatcaga ccagtgtaat actgccagcg
    21661 cggagagttt ttcttccaga agccaaaaaa atgagcatct gcctatcttt tttcggccat
    21721 tcaacctgta caggagatct ggtgccaatt ttgcttatga ggtttaatca acaatgggct
    21781 atactttgac tgactgttta acaccgaata tattcctctg atttattctt tagggataat
    21841 attataaatg taaggttaat cttaattaat tctttgctaa tattgtgtgt tgttattttt
    21901 ttgtggaagg caagccgcaa tggctaaata gggatggttg taataatcaa aacatgatac
    21961 taactatata ttgatcgttg cattgttggc atgtaacacg ctgcagcgag taacattggt
    22021 aatgaaatat aatcaacatg gataggtgaa aattaattat gagctttatg gcttttgtaa
    22081 cttgtttgac aattgcattt atcgccggtt ttgctgttgc cagaaaattg acactccgaa
    22141 aagttgcctc atcggtaatc atcgctaaat cgcaacccgt cccttcatct gcaacttccc
    22201 ctgccgctaa ggcgtcgaca aggagctata taccgcagaa aacctatcgt gactctcgtt
    22261 ggcaagagcg agatgagtta tttcagggaa tggttgccga aggtatttta ttggaaagaa
    22321 aagagtggtt aatcacgcgt tgtgaaaaaa tgtatttcga taatctggaa aaatggtttg
    22381 gacattattg tcatatccat tgccaggtct ctctggggcg cttgatcaac tttccagaac
    22441 aacctgagtt tagcgaagaa gaaagaaagc gtttctttgc aatttataat ggcatggcaa
    22501 tggattatgt tctggtttca aagaagaaca ataaaatagt ctgtgttatt gagctggatg
    22561 acccgagtca tgaaagggcg gatcgcatta ttcgtgaccg ttggttggat acaattatga
    22621 ctcttgcacg ggtgcctttt gtacgagctt ctgtcactaa attggaggaa attcctccgg
    22681 tctgggagac tcgtgacaaa attcgcccat tagtcgcggc gacaaagagt gaaccggtgc
    22741 attaactaag catcccggtc ggggcttctc atccccccgg tgtgtgcaat atacgaaaaa
    22801 aagcccgtac cttcgtacga gctcttcttt aaagatggcg gtgagggggg gattgactcg
    22861 cttcgctcgc cctgcgggca gcccgctcac tgcgttcacg gtctgtccaa ctggctgttg
    22921 ccagttgtcg aaccccggtc ggggcttctc atccccccgg tgtgtgaaat atacgaaaaa
    22981 aaagcccgta ctttcgtacg agctcttctt taaatatggc ggtgaggggg accgcctgaa
    23041 ataaatctga cttaatccag gggtgttata taatttcatt ataaacaatg tattacactt
    23101 tttgttgtcc gatattatct aatgtattct tagtgaagcg agagactaat ggcatactgg
    23161 atggcatacc agagtgacgg gaattagcgt atggcagtat tgacggatac gaaagcaaga
    23221 catatcaaac ctgatgacaa accattgccc catgggggaa ttaccggact gacccttcat
    23281 ccttcttcag taaaggggcg ggggaaatgg gtttttcgtt atgtaagtcc ggtgacacaa
    23341 aaaagacgta atgctggatt gggaacttac ccagaggtca gtattgctga agctgcacgt
    23401 actgcccgga taatgcgaga gcaacttgct gcaggtgatg atcctctgga gattaaaaag
    23461 gctgaatctg agaaagtcgc tatcccaaca tttgccgatg cagccaggcg tgtacatgca
    23521 gaactgtctc ctggatggga aaatccaaag catgtaaggc agtggttatc gacgcttgag
    23581 aattacgcgt ttcctcaact gggagcaaaa acgctggatt cgattacggc tgcggacgtg
    23641 gcagaaacac tgcgtccagt ctggttaacc ttgtcagaaa cggcaagccg ggttaaacag
    23701 cgcattcatg ttgttatgca gtggggatgg gcgcacggtt tttgtgtagc aaatcctgtt
    23761 gatgtggttg accatttgct tcctcagcag acaagaggac gtgatgaaca ccaacccgca
    23821 atgccctgga ggcagttacc gctttttgtg gcgaccagtg tgtataccga tgaaccttat
    23881 aatgttaccc gcgcactgtt attaatggtg atacttacag caactcgctc gggcgaagcc
    23941 aggggaatgc gctgggctga aattgatttt cataagcggg tatggactat acctgcagaa
    24001 agaatgaaag ccaggataca gcatcgtgtt cctttatccc ggcaggctat ttacattctg
    24061 gaaaatatac gtggcctgca tgatgaactg gtgttccctt cacccagaaa gcagcagatc
    24121 ctttccgata tggtgttgac aagttttctg cgtaaaaaga aagccgtcag tgacattccg
    24181 gggcgagttg ccacggcaca tggttttcgc tcaacattca gggactggtg tagcgaacag
    24241 gggtattcgc gggatctggc ggaaagggcg ctcgctcata cgctgaaaaa taaggttgag
    24301 gcggcatatc atcgtactga tctactggag cagcgtgtac cgatgatgca ggcatgggcg
    24361 gattatgtga tgtctcaaat tgtgaataaa taatagtgaa aatgtatatt gtacgatcat
    24421 gatttgatat gtgatcgtag cagtagtaat taagaaac
//
LOCUS       DAZFDP010000064        24202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFDP010000064 DAZFDP010000000
VERSION     DAZFDP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..355)
                     /locus_tag="ACHJ1G_002605"
     CDS             complement(<1..355)
                     /locus_tag="ACHJ1G_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764444.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSIN
                     IFSVSASLYVFSAFSV"
     gene            496..2928
                     /gene="hsdR"
                     /locus_tag="ACHJ1G_002606"
     CDS             496..2928
                     /gene="hsdR"
                     /locus_tag="ACHJ1G_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO4764445.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2995..4464
                     /locus_tag="ACHJ1G_002607"
     CDS             2995..4464
                     /locus_tag="ACHJ1G_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4764446.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4464..6233
                     /locus_tag="ACHJ1G_002608"
     CDS             4464..6233
                     /locus_tag="ACHJ1G_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4764447.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
     gene            6454..6795
                     /gene="symE"
                     /locus_tag="ACHJ1G_002609"
     CDS             6454..6795
                     /gene="symE"
                     /locus_tag="ACHJ1G_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO4764448.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(6842..8005)
                     /locus_tag="ACHJ1G_002610"
     CDS             complement(6842..8005)
                     /locus_tag="ACHJ1G_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO4764449.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(8053..8935)
                     /locus_tag="ACHJ1G_002611"
                     /pseudo
     CDS             complement(8053..8935)
                     /locus_tag="ACHJ1G_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(9032..9196)
                     /gene="yjiS"
                     /locus_tag="ACHJ1G_002612"
     CDS             complement(9032..9196)
                     /gene="yjiS"
                     /locus_tag="ACHJ1G_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4764450.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            9373..10785
                     /gene="yjiR"
                     /locus_tag="ACHJ1G_002613"
     CDS             9373..10785
                     /gene="yjiR"
                     /locus_tag="ACHJ1G_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4764451.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(11027..11971)
                     /locus_tag="ACHJ1G_002614"
     CDS             complement(11027..11971)
                     /locus_tag="ACHJ1G_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO4764452.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            12438..13670
                     /gene="mdtM"
                     /locus_tag="ACHJ1G_002615"
     CDS             12438..13670
                     /gene="mdtM"
                     /locus_tag="ACHJ1G_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4764453.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            13711..14991
                     /gene="yjiN"
                     /locus_tag="ACHJ1G_002616"
     CDS             13711..14991
                     /gene="yjiN"
                     /locus_tag="ACHJ1G_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4764454.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            15107..16258
                     /gene="yjiM"
                     /locus_tag="ACHJ1G_002617"
     CDS             15107..16258
                     /gene="yjiM"
                     /locus_tag="ACHJ1G_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4764455.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGASFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            16268..17035
                     /gene="yjiL"
                     /locus_tag="ACHJ1G_002618"
     CDS             16268..17035
                     /gene="yjiL"
                     /locus_tag="ACHJ1G_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4764456.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            17032..17289
                     /gene="ytiA"
                     /locus_tag="ACHJ1G_002619"
     CDS             17032..17289
                     /gene="ytiA"
                     /locus_tag="ACHJ1G_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4764457.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            17354..18214
                     /gene="yjiK"
                     /locus_tag="ACHJ1G_002620"
                     /pseudo
     CDS             17354..18214
                     /gene="yjiK"
                     /locus_tag="ACHJ1G_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            18282..19460
                     /gene="yjiJ"
                     /locus_tag="ACHJ1G_002621"
     CDS             18282..19460
                     /gene="yjiJ"
                     /locus_tag="ACHJ1G_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764458.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(19473..20027)
                     /gene="kptA"
                     /locus_tag="ACHJ1G_002622"
     CDS             complement(19473..20027)
                     /gene="kptA"
                     /locus_tag="ACHJ1G_002622"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO4764459.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            20277..20960
                     /gene="yjiH"
                     /locus_tag="ACHJ1G_002623"
     CDS             20277..20960
                     /gene="yjiH"
                     /locus_tag="ACHJ1G_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4764460.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            20957..21418
                     /gene="yjiG"
                     /locus_tag="ACHJ1G_002624"
     CDS             20957..21418
                     /gene="yjiG"
                     /locus_tag="ACHJ1G_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4764461.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            21431..22603
                     /gene="iadA"
                     /locus_tag="ACHJ1G_002625"
     CDS             21431..22603
                     /gene="iadA"
                     /locus_tag="ACHJ1G_002625"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4764462.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            22668..23580
                     /gene="hypT"
                     /locus_tag="ACHJ1G_002626"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             22668..23580
                     /gene="hypT"
                     /locus_tag="ACHJ1G_002626"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(23573..23965)
                     /gene="iraD"
                     /locus_tag="ACHJ1G_002627"
     CDS             complement(23573..23965)
                     /gene="iraD"
                     /locus_tag="ACHJ1G_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4764463.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(23962..24045)
                     /gene="idlP"
                     /locus_tag="ACHJ1G_002628"
     CDS             complement(23962..24045)
                     /gene="idlP"
                     /locus_tag="ACHJ1G_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4764464.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         6134 a         5999 c         5916 g         6153 t
ORIGIN      
        1 atactgaaaa agcactaaaa acgtatagag aggcactgac tgaaaaaata ttaattgaca
       61 acactaaaaa caaaaatata aaacacgcaa taagtgcaca ccaggcagca caactattca
      121 acctgttttt attactgaac ttaacaaaat accccatata ttctgtaatg cgattttctt
      181 ccactaatgc ataaagaaga ataccaacaa agcacagaga taaaataata tctttccaca
      241 gatatttata agggagaagt ataaaaccaa gaaatagata aattagcaga atgacatcta
      301 atgccttagt acaaaaatat tgtttcactc cttgcaagaa ccgaacattc tccatatgaa
      361 tccttatcct actattgatg aaactcaaga atacctttaa aacagtgact taacgctgat
      421 aatactcttt gaacagatga attcatctat ttttctgtgt agaatttgcg catcgcattt
      481 agatgagaac accccatggc ggaactgaac ctaagtaacc tgacggaagc agacatcatt
      541 accaaatgcg ttatgccagc cattctcaat gcaggctggg acaacacaac gcaaatcaga
      601 caggaggtca aactccggga cggtaaagtc atcgtacgtg gtaaagttgc ggcacgcaga
      661 acggtaaaat ctgctgacat cgtgctgtat cacaaaccag gcattccctt agccgtgatt
      721 gaagcaaaag ccaacaaaca tgaaattggc aaagggatgc aacagggcat tgaatatgcg
      781 cgcctgctgg acgttccttt tgtttttgcc accaacgggg atggctttat cttccgcgat
      841 gccaccgcag ccgaaggtga atgccttgaa aagcaaataa cgctggatga cttcccctcc
      901 cccgctgaac tctggcaaaa attctgcctc tggaaaggat acacacaagc tcagcttccg
      961 gtgattactc aggactatta cgacgatggc agcggtaaat cgccacgtta ttaccagctt
     1021 caggcaatca acaaaaccat tgaagccgtc tccaacgggc aaaaccgcgt tctgctggtc
     1081 atggcgaccg gaacagggaa aacctatacc gcattccaga tcatctggcg cctgtggaaa
     1141 tcaaaaaata aaaaacgcat tttgttcctt gccgatcgca acattctggt cgaccaaacc
     1201 aaaaataatg atttccagcc atttggtacg gcaatgacca aagtcagcgg acgcaccatt
     1261 gatcccgctt atgaaattca cctcgcgctc tatcaggcta taactggccc ggaggaagac
     1321 caaaaagcgt ttaaacaagt cgcaccagat ttcttcgatc tgatcgtgat cgacgaatgc
     1381 catcgcggca gcgcatccga agacagcgcc tggcgagaga tccttgatta cttcagttcc
     1441 gccacccaaa ttggcttaac cgccacgcca aaagagacgc atgaagtctc cagcacggat
     1501 tacttcggcg atccggttta cgtctactcg ctaaaagaag ggatcgaaga cggcttcctc
     1561 gccccttata aagttgtccg tgttgatatt gatgttgatc tgcaaggctg gcgcccaacc
     1621 aaagggcaaa ctgacttaaa cggcgaagtg atcgacgatc gtatctataa ccagaaagat
     1681 ttcgatcgca cgatggtaat cgacgaacgg acagaactgg ttgccagaac cattaccgac
     1741 tacctcaagc gcaccaaccc gatggataaa accatcgtct tctgtaacga catcgatcat
     1801 gcagaacgta tgcgccgcgc cctggtgaat ctcaacccgg agcaggtgaa aaagaacgac
     1861 aaatacgtca tgaaaatcac cggcgatgat gaaattggca aagctcagtt ggataacttc
     1921 atcaacccga aaaaagcgta cccggttatc gcgaccactt cagagctgat gaccactggt
     1981 gtggatgcta aaacctgcaa actggtagta ctggaccaga acatccagtc gatgaccaaa
     2041 ttcaagcaaa ttatcggtcg tggtacacgc atcgacgaac gttacggcaa actctggttt
     2101 accatcctcg actttaaaaa agccaccgaa ctgtttgccg atgagcgttt cgatggcatt
     2161 cccgaaaaag tcatggatac cacaccagag gatatcgccg atccagaatc tgattttgaa
     2221 gagaaactcg aagaaatcag cgaacatgac gacgaacagg taacaggcgt tgatgaaccg
     2281 cctgcgccac cataccaggt taccgatacc gatgatgtcg gcccacttcc ggaagaagac
     2341 gagaagaaaa tccgcaagtt tcacgtcaac ggtgtagcgg tgggcgttat tgcgcagcgt
     2401 gttcagtatt acgatgccga cggtaaactg gttaccgaat cttttaaaga ttacacccgc
     2461 aaaacactgc tcaaagaata tgcctcgctg gatgacttta cccgcaagtg gcaggacgcc
     2521 gatcgcaaag aagcgatcat tcacgagctg gagcaacagg ggatcatctg ggaagtactg
     2581 gcagaagaag tcggtaaaga tctcgacccg ttcgacatgc tttgccacgt agtgtatggt
     2641 cagccgccgt taacccgcaa agagcgcgcc gagaacgtgc gcaagcggaa ctacttcaca
     2701 aaatactctg aagcagcgca agccgtgctc gataatctgc tggataaata cgccgatgcg
     2761 ggcgtacagg agatcgaaag tattcaggtg ctgaaactta aaccattcga cagcatgggc
     2821 actttaccgg agattattaa aaccggattt ggcgaccgta acgggtataa tcaggcgctc
     2881 agcgagctgg aaaacgaaat ctaccaatta ccgccccgct ctgcttaaca actctctttt
     2941 gtgccttctg caaccaacat gttgcagaag gccttgtttt tcatggaata caacatgtct
     3001 atcagttcag taatcaaatc attacaagat attatgcgca aagatgccgg tgtggatggc
     3061 gatgcgcagc gtctcggtca gctctcctgg ctgctgtttt tgaaaatctt cgatgcccag
     3121 gaagaggtgc tggaactgga gcaggataac tatcaatatc cgatcccaca gcgttattta
     3181 tggcgcagtt gggccgcaaa cgctcagggc attaccggtg attccctgct ggaattcgtt
     3241 aatgatgatc tgttcccggc gttgaaaaac ctcactgcgc ctatcgataa aaacccacgc
     3301 ggctacgtgg tgaagcaggc gttcagcgat gcctataact atatgaaaaa cggtacgcta
     3361 ctgcgccagg tgatcaacaa gctgaacgaa attgacttta ccagcgccag cgaacgccat
     3421 ctgtttggtg atatttacga acagatcctt aaagatctgc aatctgcggg caatgctggc
     3481 gaattctata cgccacgcgc cgtcactcgc tttatggtgg atcgcgttga tccgaaactc
     3541 ggcgaatcca ttatggaccc ggcctgcggt acgggcggtt ttcttgcctg cgcatttgat
     3601 catgtaaaga acaaatacgt gaagagcgtc gccgatcatc agacgctgca acaacagatc
     3661 cacggtgttg agaaaaaaca gcttccgcac ctgctggcga ccaccaatat gctgctgcac
     3721 ggcattgaag taccggtaca aattcgtcac gacaacaccc tgaacaaacc gctttcctcc
     3781 tgggatgaac aactggatgt cattgttacc aacccgccgt ttggtggcac ggaagaagac
     3841 ggtattgaga agaactttcc ggcagagatg aaaacccgcg aaacggcgga tctgttcctg
     3901 caactgattg tggaagtact ggcgaaaaac ggtcgcgcgg cggtggtatt gccggatggc
     3961 acactatttg gcgaaggcgt taaaaccaaa atcaaaaaac tgcttaccga agagtgcaac
     4021 ctgcatacca tcgtgcgttt accgaatggg gtgtttaacc cctataccgg cattaaaacc
     4081 aacctgttgt tctttaccaa aggtcagcca accaaagaga tttggttcta tgagcatcct
     4141 tatccggcgg gcgtaaaaaa ctacagcaaa accaagccga tgaagtttga agagtttcag
     4201 gcggagatcg actggtgggg taacgaggcc gatggttttg ccagccgcat cgagaatgag
     4261 caagcgtgga aagtcagcat tgatgacgtg attgcgcgta acttcaatct ggatattaaa
     4321 aacccacatc aggcggaaac cattagccat gatccggacg aactgttagc gcagtatgca
     4381 aaacagcagg cggagatcca gacgctgcgt aatcaactgc gcgatattct tggcgctgcg
     4441 ctgtctggca aggaggttaa ctaatgagtg tggaaaagct gatcgttgat catatggaaa
     4501 cctggacctc ggcgttgcaa acccgttcca ccgccgggcg cggcagctcc ggtaagattg
     4561 atttgtatgg tattaagaaa ttgcgtgagc tgattctgga actggcggtg cgaggtaaac
     4621 tggtgccgca ggatccgaac gatgaaccgg cgtcggagct gctgaagcgt attgcggcgg
     4681 aaaaagcaga gctggtgaag caaggtaaaa ttaaaaagca aaaaccgctg ccggaaatta
     4741 gcgaggaaga gaagccgttt gaattgccgg aggggtggga atggactcga ttaattaatc
     4801 ttggtatatg ggctttaggg tctggttttc caaatgtggt acagggaagc actgataaag
     4861 aaattttgat gtgcaaagtt agcgacatga atcttgaagg taacgagaaa ttcatttttt
     4921 cgacaaaaaa tacaatttca aaagacctag ctgatgaata taaaattaaa atctcagaac
     4981 ccggcaccat cattttccct aaaattggcg gagcaattgc aactaataaa cgaagaattt
     5041 tagttcagga tacagctatt gataacaact gtcttggaat aaaaccatgt gatgcaatat
     5101 cgggggagtg gttctatcta atccttaata ctctcgatat gtcgaagtat caatcaggaa
     5161 cctcaatacc cgcgataaat caaagtgtca taggtagtat accaattgcg cttccttctt
     5221 taaaaatgca agagaagatt gtttcttatg tcataaccct aatgtccctt tgcgaccaac
     5281 tggaacagca atccctgacc agtctggacg cacatcagca actggttgaa accctgttgg
     5341 gaacacttac agacagccaa aacgccgagg aactggctga aaactgggcg cgtattagcg
     5401 agcatttcga caccctattt accaccgaag ccagcgtgga tgcgttaaaa cagaccattc
     5461 tgcaactggc cgtaatgggc aaacttgtgc cgcaggatcc gaatgacgaa ccagcctctg
     5521 aactgctcaa acgaattgcg caggaaaaag ctcaactggt gaaagaagga aaaataaaaa
     5581 aacaaaaacc gttgccgcca attagcgatg aggaaaaacc gtttgaactt ccggaagggt
     5641 gggagtggtg taaatttggt ttaacatcag agtttataaa tggcgatcgt ggaagtaatt
     5701 accccaataa aaatgagtat gtttctcaag gtattccttg gatcaatact ggacatatag
     5761 aaaaaaatgg tacacttacg gttacagaaa tgaattttat aaccgaagga aaatttaatg
     5821 aattaagaag tgggaaaatt caaaaaggtg atttggttta ttgcctacga ggagcaactt
     5881 ttggtaaaac tgcctttgta atcccctatg aaacaggagc tattgcctca tcactaatga
     5941 tcattagacc atttatcaca gaaatgggag ggtatattta taattattta accagtccat
     6001 ttgggcgctc acagatttac cgctttgata atggctcagc acaacccaac ctatcagcaa
     6061 atagtgtaat gttgtattca ttcccatgcc caccattaac tgaacaatat agaatatttt
     6121 cacaagtagg tttacttcat gaactatgcg ataagcttaa aactcgtata aaaacagccc
     6181 aacaaaccca actgcacctt gcagacgcac tcactgacgc ggcgataaac taattttctc
     6241 gccctcaaaa aaccaataaa gcggctaaat ggccgcttta ttcacatcac caaaaattat
     6301 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     6361 ctgatgatat atccaggtat ttagcgcggt gcggatgtgc gccaacacac ccgcaccgct
     6421 aatcacaatc actattatcg gagaatagca gttatgactg acacgcattc tattgcacaa
     6481 ccgttcgaag cagaagtctc cccggcaaat aaccgtcaat taaccgtgag ttatgcgagt
     6541 cgctacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6601 gccggttttg ccaccggcac ggcggtagat gtcaaagtaa tggaaggctg tattgtcctc
     6661 accgcccaac cacccaccgc cgaagagagc gaactgatgc agtcgctgcg ccaggtgtgc
     6721 aagctgtcgg cgcgtaaaca aaagcaggtg caggatttta ttggggtaat taccggtaaa
     6781 cagaaagtcg cctgataaat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6841 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6901 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6961 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     7021 gcgacaaccg cagtcggctt ttttgcggta caacatcaac aaaattcgtt tcagctttaa
     7081 acgcaatata gagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     7141 cgcgaagatg atcgaataac acccggctat gcgaaccatc agcaagaaaa ggataatcac
     7201 tcaggctgta actggttttg ttttcaggct gcccccgata aacagctaaa acctcttcag
     7261 aaagagaagg cgcttgccag acctgaaccg cgagatcggc cagacgatcg gcacgggcgc
     7321 gaatggcggc ttcatcccat ttttcgcact gcccaagacc gatattcaaa tagagcgggc
     7381 tatgtttaaa gccgtcttca atatcgcgtt tttccgcgaa aggtctgtca ctgtagcggg
     7441 agttatagcg cgttaaagtg agattcccca acgtatgtaa cagttcttta tgcacacgct
     7501 gccagtcgct tcccagctct tcacgccatt tagccgacag attttcgttc tgcggcataa
     7561 tgtgctcaat cgtaaactct tccacgcgct ctctgcgttt atcgttttcc agtcttcgta
     7621 accagtagct gcggctgcgg aagttataga gatcgcgaac ttttaattcc cgtttaaatt
     7681 catcatcgtt ggggaaacga cgatatgaag gcaggttcat aaaatgtacc tgaatacttt
     7741 ccagatattt ttctttatta atgaccttat aaaaagtggc aaacgtcttg ttcaaagaat
     7801 tcgtcggaat tgcacatact gcacggcgga aaacataaga ttcaattaaa cgaattatgc
     7861 tcaggaaatc ttcgtgagac aaatcgtcat ttttatagtc atgataaagc gccagtaaga
     7921 aaggatacgc cacatcaacc tttaactcgc gcaaatcctg aaaagccgta gcaagcgatt
     7981 tgtcactttc ttttcccaat gccattgcac agtaatactc cgcgtagata tgaatatctt
     8041 caaccagccg atctacgcct ttttcagcaa cactctggct gcgggcatgg agtttaaatg
     8101 cctcatagac ttcatctgtc cgagggatct cccccgtttt taccgtcaga taatgacgca
     8161 taaaactgtc aaaatattcg ctgtaacctt gctgaccaaa ggcgacttcc attggacgcc
     8221 agtgatcttc atacaaccgg gtttgatgct ctggttcgag gcccatcaga ataaaattgc
     8281 gcaccagatc ggcctgactt aacgccttac cggtggagtt catactttca aaaatcagtt
     8341 gcggattatc ctgaccacga ttaagcgcca catcgacaat taataacttt gctaacccac
     8401 gacataaggg gatcaagtca tcaccgagtt tggcaatttg ttcatcaaag aaagtaaagt
     8461 tttccattat tcgatgggaa tagttttctg gcattggtct ttgttttatt aacgccagta
     8521 aactgtcttt atcagtctcg gtcagtagta atttaaaacc tttctcgccg gactcataag
     8581 gattgagcaa ataataatta cgcaatttca ttgctgaaaa gccatcaaaa acttcgtctt
     8641 cgccaagatg gcgcgataaa gcctcaatca gcaacattgc ggtcgtcagc cgttgttgac
     8701 catcaatgac cagtaacgga gaaataccag aaacctgata caacccctgc tcaatataaa
     8761 caaccgaacc gataaaatgc gctgatatat cgtcacgctt tccggcacga atgatgtcgt
     8821 cccaaagttg ccgacattgt tgttctgtcc atgaataagt ccgttgataa atgggaataa
     8881 caaactgctg cgaacgtttt aaaaaatcga gtaggcgggc ttctgtagct ttcattctga
     8941 cgtctttccg tgaagcgatg atttcgcaat aataaccact tccttttgga ggttatagag
     9001 aaatcctgtt tatccgcgat gaattgcccc ctcactccac atcctccctg cgtaacccaa
     9061 tatctctcaa ccgctcatca ctcatctgct gtaacactcg tcgggtctgc atttgccgcc
     9121 gccaacgcct caccgcctgc cagagttgca caagtctgat aaacggcgtt ttggctttgt
     9181 tttcgtgaaa ttccatctgc tgcctcctca tctttcagag gcttcatcat ccaccttctg
     9241 gcagttcaca atacagatgc aaatattagt tttatttaac atacagacag gttaaaacag
     9301 catctgcatc cggtttttta gcctcatctg taccggtttt tctttctgta tggtccaaat
     9361 aaggaataca gcatgacgcg ttatcaacat ctggcgactc tacttgccga gcggattgag
     9421 caagggctgt atcgtcatgg ggagaaattg ccgtcggtgc gtagcttaag tcgggagcac
     9481 ggcgtcagca tcagcaccgt gcagcaggcg tatcagacgc tggagacgat gaagctcata
     9541 accccacagc cgcgttcggg ttattttgtc gcacaacgta aagcccagcc gccagtgccg
     9601 ccgatgacgc gtccggtgca gcgcccggtg gaaattaccc agtgggatca ggtgctggat
     9661 atgctggtgg cgcatagcga cagttccatt gttccgttaa gcaaaagcac gccggatgtc
     9721 gaaacgccca gcctgaaacc gctctggcgg gagctaagcc gggtggtgca gcataatctg
     9781 caaaccgtgc tcggttatga cttgttagcc ggtcagcggg tattgagaga gcagattgcc
     9841 cgcctgatgc tcgacagcgg ctcggtggtc accgccgatg acatcattat caccagcggc
     9901 tgccataact cgatgtcgct ggcgttaatg gcggtgtgta aaccgggcga tattgtcgcg
     9961 gtcgaatctc cctgttatta cggttcgatg cagatgctgc gcggcatggg cgtgaaagtg
    10021 attgaaatcc caaccgatcc agaaacaggc atcagcgttg aagcgctgga actggcgctg
    10081 gaacagtggc cgattaaagg catcattctg gtgccaaact gtaataatcc gctgggattt
    10141 attatgccgg acgcacgcaa acgggccgtt ctctctctcg ctcagcgtca tgatattgtg
    10201 atttttgaag atgatgtcta cggcgaactg gcaacggagt atccgcgccc gcggaccatc
    10261 cattcatggg atatcgacgg gcgagtgctg ttgtgcagct cgttcagtaa aagtattgct
    10321 ccaggcctgc gcgtgggttg gggcgcaccg gggcgttatc acgataaact gatgcatatg
    10381 aaatacgcca tcagcagctt taatgtgccg tccacgcaaa tggcggcggc aacgtttgtg
    10441 ctggaaggtc actatcatcg ccatatccgg cggatgcggc agaactatca gcgcaatttg
    10501 gcgctttata cctgctggat acgggaatat tttccctgcg aaatctgtat tacgcgcccg
    10561 aaaggcggat ttttactgtg gatagaattg cctgaacagg tcgatatggt ctgcgttgcg
    10621 cggcagctgt gccgcatgaa tatccaggtg gcagcaggct cgattttctc ggcttccggc
    10681 aaataccgta attgtctacg catcaactgc gctttgccgc tcagcgaaac ctatcgcgaa
    10741 gcactaaagc aaattggcga ggccgtgtat cgggcaatgg aataaattcg aattagtggc
    10801 gaagcacctg atttcctctc acgaatcgcc tgtaactcgt tgtatctttg cagggtcatc
    10861 gccacgttcc agcatggctt aggcaaagtg tgcttcgttc ataccggatg cggcgtgaac
    10921 gccttatccg gcctacaaac cgcgcaaatt caatagattg cagaggaaat gtaggtctga
    10981 taagcgtagc gcatcaggca gctttgcgtt tgtcatccgt ctctgattag tattcgatcc
    11041 atggatcgcg cttgatttca ggcaagggct gctctaatgc ctgcatttgc tctgtcgaaa
    11101 gtccggtaat cttttgtatc cattcaggat ctgccccatt ctgcaacaac aatctaagga
    11161 ggcgttgttc cccttcttct ttcccctttt ccattcccaa cagccatcca tcattataaa
    11221 ttcgctctgc aatcgtcatt attcgctccc tgtgttgtgg cattcgacgg gtaagttcgc
    11281 tgataaactt cttaaaacgc gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag
    11341 ttatctggct gtcattagca cactctgtaa ccagtaatac taccagttgg tcgataagcc
    11401 ccatcagatc gcgctggcga atatgctttt ggatcaactc caacagggcg actctgcgat
    11461 gctgcacaat ctcgtcgtct ggcacgacag tgatatccac cagcgggaac gcggcggtat
    11521 aaagtttccg tgcggtagtt gggtcggcaa attcgtccag ccagcacagg gaccagggat
    11581 aaggactacg gctaccgtga taaaatagca tcggtataac cagcggtagc gcccgacgtt
    11641 tatcatgctc gatatggcgc tgcatcaccg ccatggaata gcgcatcagg cgaaaggcca
    11701 tatgaatatc ctcacggctc tgatgttcaa tcaccacata aatatagcca tcgccttcgc
    11761 gggtatttac cgaccacaga atatcggagt gtagcgcccg caatttttca tcgacaaagc
    11821 tggcggattc cagttttaag ctgtcgagat cgcacagttc acgtaaatct ttgggtaaat
    11881 agatctccat aaaatcccgc gcggtgtcag ggtgggtgag aaaggattta aataatgcgt
    11941 catgcggcgt gctggttgtg aagtttgtca tggcgatccg tcacctgttg ataagatggc
    12001 ccgactttag cgactaacaa aaccattcac acctggcaat ttcccgttcc ccgaggcgct
    12061 ttccaggcta tttttgttcc tctgcaagcg atacattttt ccggaaacag gctcgcgttt
    12121 tacatttcct gtacccggta gatattcccc tttccaccgc gctcttttga ttaatcgcaa
    12181 attttaatta cacttaaggt gtatattttc tatgcaaacc atcaattcaa gaggtgtaat
    12241 gtgctgatga ctatttgtaa tcgttataca ttctgacccg aactcagaag gtatttctct
    12301 gtctgtataa gcacaggcag tgtggttgat tacatgaaat tcagtacatt tgtccttctc
    12361 gcctaccttt cgtcattacc gacggtattg aatttcgttt tccccgttgg ggttctccgg
    12421 acaaggagtt gtttgttatg ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc
    12481 cgatggcgtt gattttgtat gattttgccg cgtacctgtc gacggacctg attcagccgg
    12541 ggatcattaa tgtggtccgt gattttaatg ccgatgtcag tctggccccg gcagccgtca
    12601 gtctctatct cgcgggcggt atggcgttac agtggctgct ggggccgctt tccgacagga
    12661 ttggccgcag gccggtgctg attaccgggg cgctaatatt tacccttgcc tgcgctgcga
    12721 caatgttcac aacgtctatg acacagtttc ttatcgcgcg tgcaattcag ggcaccagta
    12781 tctgttttat tgctaccgtt ggttatgtca cggtgcagga ggcgttcgga cagacaaaag
    12841 ggatcaagtt gatggcgatt atcacctcca tcgtactgat tgcgccgatt attggcccgc
    12901 tttccggcgc agctctgatg cactttgtgc actggaaagt cctttttgcc atcattgcgg
    12961 ttatgggttt tatctcattt gttggcttac tgctggcgat gccagagacg gtgaagcgcg
    13021 gcgcggttcc atttagcgcc aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc
    13081 ggctgttcct ctttggcgca gcaaccatct ctttaagcta tatcccaatg atgagttggg
    13141 tggctgtctc gccggtgatc ctgattgacg caggcggctt aacaacttcg cagttcgcct
    13201 ggacacaggt tccggtgttc ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg
    13261 ttaaagatcc gaccgaaccg cgctttatct ggcgtgccgt acccattcaa ctggtcggcc
    13321 tcgcactgtt gattatcggc aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg
    13381 gcaccagtct gtatgctttc gggattggtt taatcttccc gaccttattc cgctttacac
    13441 ttttttccaa taacttaccg aaagggaccg tctccgcatc gctgaatatg gtgatcctga
    13501 tggtgatgtc tgtctcggtc gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc
    13561 cgtttcatct gttagccgtc gtagcgggag ttatcgtcgt tttcaccctg gcggggttgc
    13621 tcaatcgcgt gcaccaacat caggctgccg agctggcgga ggagcggtga tttttgcgcg
    13681 atcctgccgt caggctctat tcttaacgtt atgaataaac tcattgaact cagacgcgcc
    13741 aaaatgttgg cgctctcttt actgcttatc gccgctgcta cctttgtcgt tacgctgttt
    13801 ttgccaccca atttttgggt gagcggcgtg aaggcaattg ctgaagcggc gatggtcggc
    13861 gcgctggcgg actggtttgc ggtggtggcg ctgtttcgcc gcgtgccaat tccgatcatt
    13921 tctcgtcata cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa tctcggtcag
    13981 ttcgtgcagg aaaaatttct cgatacccag tcgctggtgg cattgattcg acgtcacgaa
    14041 ccggcgttgc taattggtaa ctggtttagc cagccagaaa acgcccgccg cgttggtcag
    14101 catctgttgc agatcatgag tggttttctt gaactgaccg atgatgcacg tattcagcgc
    14161 ctgcttaagc gcgcggtcca tcgggcgatt gataaggtcg atctttccgg aaccagtgcg
    14221 ttgatgctgg agagtatgac caaaaacgat cgtcatcagg tgctgctcga tacgctgatc
    14281 gcacagttga ttgctcttct ccagcgcgat aaatcgcgca agtttatcgc ccaacaaatt
    14341 gttcgctggc tggagagtga gcatccactg aaagccaaaa ttctccccac cgaatggctg
    14401 ggcgaacata gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga tgatatcagc
    14461 cgcgatcgtg cgcatcagat ccgccatgca tttgatcgcg ccacttttgc cctgatcgac
    14521 aagttgaaaa acgatccgga aatggcagcg cgagctgatg ccgtaaaaag ctatctgaaa
    14581 gaggatgaag cctttaaccg ctatctcagt gaattgtggg gggatttacg ggaatggctg
    14641 aaagcggata tcaacagtga agattctcgt gtgaaagaac gtatcgcgcg ggcgggtcaa
    14701 tggtttggcg aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa tggtcatctg
    14761 gaacaagccg cgcaccgcgt cgcacctgag ttttccgcat tcctgacgcg ccacatcagc
    14821 gatacggtaa aaagctggga tgcccgggat atgtcgcggc aaatagagtt aaatatcggc
    14881 aaagatctgc agtttatccg tgtcaacggt acgctggttg gcggttgtat tgggctaatt
    14941 ttatatttgc tgtcacagct cccggccttg ttccccctcg gcaattttta gaaatccatg
    15001 aaaaactaag tggctaaccg cgagagagat caaataaacc acactaaaag aatggtaata
    15061 atgtggcctc ttttcattat ctcccgtggt acggggaagg aaaatcatgt cacttgtcac
    15121 cgatctaccc gccattttcg atcagttctc tgaagctcgc cagaaaggct ttctcaccgt
    15181 catggatctc aaggagcgcg gcattccgct ggttggcact tactgcactt ttatgccgca
    15241 agagatcccg atggcagccg gtgcggttgt ggtttcgctc tgttccacct ctgatgaaac
    15301 cattgaagaa gcggagaaag atctgccgcg caacctctgc ccgctgatta aaagcagcta
    15361 cggcttcggt aaaaccgata aatgccccta cttctacttt tctgatctgg tggtcggtga
    15421 aaccacctgc gacggcaaaa agaaaatgta tgaatacatg gcggagttta agcctgttca
    15481 tgtgatgcag ctgccaaata gcgttaaaga cgatgcctcg cgtgcgttat ggaaagccga
    15541 gatgctgcgc ttacaaaaag cggtggaaga acgttttggg cacgaaatta gcgaagatgc
    15601 tctgcgcgat gccattgctc tgaaaaaccg cgaacgtcgc gcactggcta atttttatca
    15661 tcttgggcag ttaaatcctc cggcgcttag cggcagcgac attctgaaag tggtttacgg
    15721 cgcaagcttc cggttcgata aagaggcgtt gatcaatgaa ctggacgcga tgaccgcccg
    15781 cgttcgtcag cagtgggaag aaggccagcg actggacccg cgtccacgca ttttaatcac
    15841 cggctgcccg attggcggcg cagcagaaaa agtggtgcgc gcgattgaag agaatggcgg
    15901 ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg accgagcaat gcgtggcaga
    15961 aacgggcgat gtctacgacg cgctggcgga taaatatctg gcgattggct gctcctgtgt
    16021 ttcgccgaac gatcagcgcc tgcaaatgct cagccagatg gtggaagaat atcaggtcga
    16081 tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat cgctggcgat
    16141 taaacgtcat gtgcgtcagc agcacaacat tccttatatc gctattgaaa cagactactc
    16201 cacctcagat gtcgggcagc tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg
    16261 agtggcagtg acatattcga ttggcattga ttctggttct accgccacca aagggatctt
    16321 actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc gtccggcaac
    16381 agcaattact gaagcctggg aaactctgcg cgaagggtta gagacaacgc cgtttctgac
    16441 gctcaccggc tatggacggc aactggtgga ttttgccgat aaacaagtga cggaaatctc
    16501 ctgtcatggg ctgggcgcac ggtttcttgc gccagcaacg cgcgcggtaa tcgacatcgg
    16561 tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg atttcctgat
    16621 gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg
    16681 caccagcgtc gagcaactcg acagcattac cgaaaatgtc acgccgcacg ccatcacgag
    16741 tatgtgcaca gtgtttgctg aatcagaagc gatcagcctg cgctcagcgg gcgtcgcgcc
    16801 agaagcgatt ctcgccggag tgattaacgc gatggcgcgg aggagtgcca atttcattgc
    16861 tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagtcatt gccagacgtt
    16921 tgcccggatg ctggaatctc atctgggaat gccggtaaat acccatcctg atgcgcaatt
    16981 tgctggcgca attggcgcgg cggtaattgg tcaacgagtg aggacacgcc gatgaaagag
    17041 tttttatttt tatttcactc cacagtcggc gtcatacaaa cccgcaaagc gttgcaggca
    17101 gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta
    17161 tgcatttggc tgacctgtcc ccccggcgag gaaatgcaat gggtgatccc tgggctgact
    17221 gagtcaattt attgtcagca ggatggtgtc tggcgctgca ttgcacatta ccgggtttct
    17281 ccgcgttaag gattcattaa ggtcgctacg tatacttctc gatatcttat tgatttgcct
    17341 taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatctccg ttattgtgat
    17401 tctttttgcc attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa
    17461 tcatgcagca taatttcaga attaccacgc caccattgac ggcaaagaaa ttgcaggcat
    17521 aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt ttagcactat
    17581 caacaaacct gccgccattg ttgaaatgac caccaatggt gattttatcc gcacaattcc
    17641 tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc agtttgtcat
    17701 tagcgatgag cgcgattacg ccatttatgt gatttcactg accccaaact cggaagtaaa
    17761 aatcctcaaa aaaataaaaa tcccgctgca agactcccca accaattgcg gttttgaagg
    17821 gttggcttat tctcgccagg atcacacttt ctggtttttt aaagagaaaa atccgattga
    17881 ggtctacaaa gttaacgggc tgttaagtag caatgaatta cacatcagca aagacgaggc
    17941 actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa
    18001 tacgcttctg gtgctgtctc atgaatctcg cgcactccag gaagtaaccc tggttggcga
    18061 ggttatcggt gaaatatcac taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca
    18121 ggccgaaggg attgcgatgg atgcctctgg taatatttat atcgttagcg aaccaaatcg
    18181 tttttatcgc tttaccccta aatcatcgca ttaacaggct ggcatatttc acctttttgt
    18241 tattaacttg ttaacaacaa aaatgacata caaggttaaa catgccttcg tcctcgcatc
    18301 ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg accttaggta
    18361 tgggattagg tcgcttcctt tatacgccta tgttgcccgt catgatggcg gaaggggcgt
    18421 tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat ctggctggca
    18481 gtctgctatt ttcgtttggc gcatttcacc agccgtcgcg cctgcgccca tttctgttgg
    18541 tttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgccg ccatttattc
    18601 tggtgttatt gattcgcttc ctggcgggtg tcgccagcgc cggtatgctg atttttggtt
    18661 cgacgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg ttttctggcg
    18721 ttggcattgg cattgcactg ggcaacgaat atgttctggc aggcctgcat tttgccttat
    18781 cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg attgcactta
    18841 cgcttttaat gccctcgaaa aaacacgcca tcgcaccaat gccattggca aaaacggagc
    18901 aacagataat gagctggtgg ttactggcta ttctgtacgg cctggcggga tttggttata
    18961 tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg
    19021 cccatctctg gacgttagtc ggcttatcaa ttgtgccagg ctgctttggc tggctatggg
    19081 cagcaaaacg ttggggggct ttgccttgcc tgacggcgaa tttgctggtg caggctatca
    19141 gtgtgctgct tactctcgcc agcgactctc ctctcttgct tatcattagt agtatcggtt
    19201 ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc caactgagcg
    19261 ttccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt ggacaaatcc
    19321 tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc ggcgccactc
    19381 tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtacaa ttatttaagc
    19441 tgcaagtggt cacttcatga ggagtgtttc ccttaccact caagaaattc gaccgcaacc
    19501 gttgatgtca gccagacccc gttttccgtt tgccagaaag ggatgcctcg tttcgccatt
    19561 tcctgtgctt tgacggttaa gataaccggc gatccgtgtc ttgctcccac tttgcgtgcc
    19621 gttgcttcat cagcagagag atgtacgtag tgacgctcac ctgcaatcag cccctgtttt
    19681 tttatctcgt caaggaaacg gcttgccgta ccgtggtaaa gaaactgtgg cggtgttttt
    19741 tcggcaaagg aaatagcgac ctgcgaagtc aaatgcccct gaaccgcgcg gatgcaccta
    19801 ccatcactgg aataactaaa acgcttttta tcactggtgg cgaccacggt atccagcaat
    19861 gtacgggtta atcgcttgcc cgctttttgg gcacaaagta tgagcttatc gatatccgcc
    19921 catccttcac ggtcaagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
    19981 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatctc gttttcgtcc
    20041 ctggattttc tgcttttatt tactgatgtc attgtcactg ggtttatgaa ttttctgcaa
    20101 ctcaatcctg cgcagagcct tttccacaca gcgtaatttt tccgctatgc aaaaaacgca
    20161 tagccagaaa catacttgca ttttccccat acccacatgg ccgataaggt ttttcagctc
    20221 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
    20281 cacaacaagg ggatgctgta gccggggatc tggcgacaga aaaagtgggc attaaagggt
    20341 atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccggg acagacagct
    20401 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
    20461 acggagccac cacctcattt cgcggggcgg gtggcgcggg ggctaaagat ggctttctct
    20521 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
    20581 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
    20641 gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacact gatgccgccg
    20701 ccggtatgac caaagagctg gcgcaggaag gggaaattac cgaacgcgat aaagtgattt
    20761 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
    20821 cggtgttcgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
    20881 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
    20941 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
    21001 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
    21061 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
    21121 tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc ttttagccgc
    21181 attaatgagt atgggtggcg cagtcggtgt tgccgccagt ctggcaaccg ctggcgcatt
    21241 aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca acccggtgca
    21301 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
    21361 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
    21421 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
    21481 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
    21541 cgttgccagc aatatccctt ctgacattgt accggactgc acggttgtcg atctcagcgg
    21601 gcagatcctc tttcctggtt ttattgatca acacgtccat ttgattggtg gtggcggtga
    21661 agcaggtccc acgacgcgca cgccagaagt ggcgctaagt cgcctgacgg aagcgggcgt
    21721 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
    21781 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
    21841 tcatgtccct tcccgcacca ttacaggttc cgtggaaaaa gacgtggcga ttatcgatcg
    21901 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
    21961 cctggctaat atggcggcgg aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
    22021 caccgtgttc cacatgggcg acagcaaaaa ggcgttacag cctgtctatg acctgctgga
    22081 aaactgcgat gtgccgatca gcaagttgct gccgacccac gttaaccgca acgttccgtt
    22141 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcact atcgatatca ccagcagcat
    22201 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
    22261 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
    22321 gaatttaacc catatcggtg ttgccggttt tgaaacgttg ctggaaaccg tgcaggtgct
    22381 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
    22441 cggtttcctt aacctgaccg ggaaaggcga aattctgcca ggcaatgatg ctgacttgct
    22501 ggtcatgacg ccagaactgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
    22561 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
    22621 gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg gatgactgtg
    22681 gtgcgatttt gcataatatt gaaaccaaat ggctttatga ttttctgacc ctggaaaaat
    22741 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
    22801 gcatccgtgc gctggaacag gcgattggtg ttgaattgtt taaccgccag gtaacgccgc
    22861 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
    22921 tagagagcaa tctggcagag ctgcgtggcg gcagcgatta cgcgcaacgt aaaatcaaga
    22981 tagccgctgc acactctctt tccctcgggc tgttaccgtc cattatcagc cagatgccgc
    23041 cgctctttac ctgggcaatt gaagctattg atgtcgatga agcggtcgat aaactgcgtg
    23101 aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
    23161 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
    23221 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
    23281 acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
    23341 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
    23401 cctggctgcc ggaatacgct atccaacaag aaattcgcag cgggcagctc gttgtgctga
    23461 atcgggacga actggtgatc ccgatccagg atctacgcat accggatgaa tactcgcatg
    23521 aatcccgttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt gcttagctga
    23581 cattctccag catcgcacta caccaaccgc cattatccca gaccagatca aacgttagcg
    23641 gttcaccgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg cagtacgctt
    23701 ctttaaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt gcagaacgca
    23761 gctgacggca ataccagctc tggagatgtt tgtcgctctg gttatgcact tgccactcgg
    23821 gcatgcctga aacaagtaat gacggtaatg acgaatgagg agaattaaaa agagtgagga
    23881 gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt tcaggtaaaa
    23941 cagcctgacg tgattggcgc atcattttgc gcactcctga cgtttagcaa catatgcggc
    24001 ttgccctgaa caccgggcac cactgtattg atgctcattt tccatagcca ttcccttttg
    24061 ttcattacat ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag tataaaacct
    24121 tacttgccta tgaatatcta ctgacttttc aggaaaaaag tcatgatttg ataaaaatca
    24181 tcaaactcaa aaaaaaaccg ct
//
LOCUS       DAZFDP010000065        23941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFDP010000065 DAZFDP010000000
VERSION     DAZFDP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..1478)
                     /gene="xylB"
                     /locus_tag="ACHJ1G_002629"
     CDS             complement(24..1478)
                     /gene="xylB"
                     /locus_tag="ACHJ1G_002629"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4764465.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1550..2872)
                     /gene="xylA"
                     /locus_tag="ACHJ1G_002630"
     CDS             complement(1550..2872)
                     /gene="xylA"
                     /locus_tag="ACHJ1G_002630"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4764466.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3238..4230
                     /gene="xylF"
                     /locus_tag="ACHJ1G_002631"
     CDS             3238..4230
                     /gene="xylF"
                     /locus_tag="ACHJ1G_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4764467.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4308..5849
                     /gene="xylG"
                     /locus_tag="ACHJ1G_002632"
     CDS             4308..5849
                     /gene="xylG"
                     /locus_tag="ACHJ1G_002632"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4764468.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5827..7008
                     /gene="xylH"
                     /locus_tag="ACHJ1G_002633"
     CDS             5827..7008
                     /gene="xylH"
                     /locus_tag="ACHJ1G_002633"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4764469.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7086..8264
                     /gene="xylR"
                     /locus_tag="ACHJ1G_002634"
     CDS             7086..8264
                     /gene="xylR"
                     /locus_tag="ACHJ1G_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4764470.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8372..9196)
                     /gene="bax"
                     /locus_tag="ACHJ1G_002635"
     CDS             complement(8372..9196)
                     /gene="bax"
                     /locus_tag="ACHJ1G_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4764471.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9516..11546
                     /gene="malS"
                     /locus_tag="ACHJ1G_002636"
     CDS             9516..11546
                     /gene="malS"
                     /locus_tag="ACHJ1G_002636"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4764472.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11724..12977
                     /gene="avtA"
                     /locus_tag="ACHJ1G_002637"
     CDS             11724..12977
                     /gene="avtA"
                     /locus_tag="ACHJ1G_002637"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4764473.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13129..13602)
                     /gene="ysaA"
                     /locus_tag="ACHJ1G_002638"
     CDS             complement(13129..13602)
                     /gene="ysaA"
                     /locus_tag="ACHJ1G_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4764474.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13704..14552)
                     /gene="yiaJ"
                     /locus_tag="ACHJ1G_002639"
     CDS             complement(13704..14552)
                     /gene="yiaJ"
                     /locus_tag="ACHJ1G_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO4764475.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14753..15751
                     /gene="yiaK"
                     /locus_tag="ACHJ1G_002640"
     CDS             14753..15751
                     /gene="yiaK"
                     /locus_tag="ACHJ1G_002640"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO4764476.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15763..16230
                     /gene="yiaL"
                     /locus_tag="ACHJ1G_002641"
     CDS             15763..16230
                     /gene="yiaL"
                     /locus_tag="ACHJ1G_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO4764477.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16348..16821
                     /gene="yiaM"
                     /locus_tag="ACHJ1G_002642"
     CDS             16348..16821
                     /gene="yiaM"
                     /locus_tag="ACHJ1G_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO4764478.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16824..18101
                     /gene="yiaN"
                     /locus_tag="ACHJ1G_002643"
     CDS             16824..18101
                     /gene="yiaN"
                     /locus_tag="ACHJ1G_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO4764479.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18114..19100
                     /gene="yiaO"
                     /locus_tag="ACHJ1G_002644"
     CDS             18114..19100
                     /gene="yiaO"
                     /locus_tag="ACHJ1G_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO4764480.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19104..20600
                     /gene="lyxK"
                     /locus_tag="ACHJ1G_002645"
     CDS             19104..20600
                     /gene="lyxK"
                     /locus_tag="ACHJ1G_002645"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO4764481.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20597..21259
                     /gene="ulaD"
                     /locus_tag="ACHJ1G_002646"
     CDS             20597..21259
                     /gene="ulaD"
                     /locus_tag="ACHJ1G_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4764482.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            21252..22112
                     /gene="sgbU"
                     /locus_tag="ACHJ1G_002647"
     CDS             21252..22112
                     /gene="sgbU"
                     /locus_tag="ACHJ1G_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO4764483.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22106..22801
                     /gene="araD"
                     /locus_tag="ACHJ1G_002648"
     CDS             22106..22801
                     /gene="araD"
                     /locus_tag="ACHJ1G_002648"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4764484.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22833..22934)
                     /locus_tag="ACHJ1G_002649"
                     /pseudo
     CDS             complement(22833..22934)
                     /locus_tag="ACHJ1G_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22938..23201
                     /locus_tag="ACHJ1G_002650"
                     /pseudo
     CDS             22938..23201
                     /locus_tag="ACHJ1G_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23148..23888)
                     /gene="yiaT"
                     /locus_tag="ACHJ1G_002651"
     CDS             complement(23148..23888)
                     /gene="yiaT"
                     /locus_tag="ACHJ1G_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4764485.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
BASE COUNT         5897 a         6157 c         6051 g         5836 t
ORIGIN      
        1 cccggtcagg caggggataa cgtttacgcc attaatggca gaagttgctg atagaggcga
       61 cggaacgttt cacgtcgtgg ctgataagcg gcataacgct gcgcatctgg tagatgcgac
      121 tgttctaacg gtagctgcgg caacaactca atgagcgatt tctctggatt cgccgcaatc
      181 tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc cccccgtacg gtaatcgagc
      241 tgctgaccgc tgatatccgc cagcatctga cgccagtact cactacgcgc cccgccacca
      301 atcaacgtaa cactttgcgg tttaatgccg caggcatgca cgacatccat gccatctgcc
      361 agcgcataac ccacgccttc cagcactgct cgcgccagtt cattggggcc atgttgatga
      421 gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt gtggtgtacg ctcgccggaa
      481 agataaggca gaaaccaaac tggctcggca ctttcatcag cctgttgagc tgcagcgatt
      541 aaagctggga cattgcacag gccggttaat ttcgcgaccc aatccagaca cgatgctgca
      601 ctcagcatta cagacattaa atgccaacgc tgcggtagcg catggcaaaa gctgtgtaca
      661 gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa aatagacccc cgacgtcccc
      721 agcgataaca ttgcctgatt agcatcaacc attcccacac caactgcacc agctgcattg
      781 tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc acgctttcgc aacttcaggt
      841 aacaaagcac cagtaatttc gctgccttcg tataatgcgg gcatctggtc acgagataag
      901 tcgcaagcct gcagcatgac gtcactccag tcacgctttg cgacatccag ccacatggtg
      961 ccagctgcgt cagacatatc gctggcaaac tcccccgtca tacgcagacg caagtaatct
     1021 ttcggtaata atactttgtc gatttgacgg aatatctccg gctcatgccg ctgaacccat
     1081 agcaatttag gcgcagtaaa tccgggcatc atcaggttgc cggtaatcac tcgtgattgc
     1141 ggaactctcg cttccagcaa agtgcactct tgcgcacagc gcccgtcgtt ccacaaaatg
     1201 gcagggcgca atacccgttg ttgagcatcc agtaaggttg ctccatgcat ctggccggca
     1261 atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc ccagagcttt cattgcgcga
     1321 tcagttgcct gccaccactg ttccgggtct tgttccgacc agagtggatg cgggcgcgaa
     1381 acggtcagct tttccgtttg cgaagcaacc acctcaccct gctcgttgag caaaataact
     1441 tttacgcccg aggtgccaag atctatcccg atatacatat cgatcgttcc ttaaaaaaat
     1501 gcccggtatc gctaccgata accgggccaa cggactgcac agttagccgt tatttgtcga
     1561 acagataatg atttaccaga ttttccagtt gctcctggcg accactctga tgcaccggag
     1621 acaaattatg ttcctgagca tatttggcta aatctgccag tgacatttgg cctttcagga
     1681 tttgctggcc caattcgcta ttccagccgg aataacgctg cgcgatgcgt ttatccagct
     1741 cgccatcttc aatcatgcgc gctgcaattt tcagcgccac tgccatcgta tccatcgcgc
     1801 cgatatgacc gtaaaacaga tcatatttat cagtactttg acgacgtact ttggcatcga
     1861 agttcagacc accggtggtg aaaccacctg ctttgagaat ttcatacatc accagcgcat
     1921 tctcttccac actgttcggg aactggtcgg tgtcccagcc cagttgcgca tcgccacggt
     1981 tggcgtcgac agaaccgaac aggccaagcg caatggcggt ggctatttca tgatggaaag
     2041 agtgacctgc cagcgtcgcg tggttagctt caatgttcag tttaatctct ttttccagac
     2101 caaactgttt caggaagcca tagaccgtcg cggcatcgta atcatattga tgtttagtcg
     2161 gttcttgcgg tttcggttcg ataagcaacg tgccctggaa gccgatttta tgtttatgct
     2221 caaccaccat ctgcataaag cggcccagtt gttcacgctc ctgacgcaag tcggtattta
     2281 acagcgtttc gtaaccttca cgaccacccc acaggacata gttttcaccg cccaatttat
     2341 gggttgcttc catcgctgta acaacttgcg ttgccgccca gctgaagact tcaggatctg
     2401 ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa gcagttagcg gttccccaca
     2461 gcagcttcac gccgctctct tcttgcttgg ctgccaggac atcaaccatt tgcgcaaaat
     2521 tattgatgta ctcttttaac gacgcgccct caggggaaac atccacatcg tggaagcaat
     2581 aaaatggcac atgtaacttg tggaaaaact caaatgcgac atctgcttta cgcttcgcca
     2641 acgccagtgc ctcaccaggc tgctgccacg gacgattaaa cgcccccaca ccaaacatgt
     2701 ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc aaaacgcaag tgctcttcca
     2761 tacgcttacc caacaccagt tcgtcgggat tgtagtgacg gaatgctaac gggtttgagg
     2821 attttgagcc ttcataacga acgcgatcga gctggtcaaa ataggcttgc atattgaact
     2881 ccataatcag gtaatgccgc gggtgatgga tgatgtcgta atattgggca ctccctttca
     2941 gttgctcaat tatgttattt cacactgcta ttgagataat tcacaagtgt gcgctcgctc
     3001 gcaaaataaa atgggatgat gaaactgggt aattcctcga agataaaaat gcaataagta
     3061 caattgcgca acaaaagtaa gatctcggtc ataaatcaag aaataaacca aaaatcgtaa
     3121 tcgaaagata aaaatctgta attgttttcc cctgtttagt tgctaaaaat tggttacgtt
     3181 tatcgcggtg attgttactt attaaagctg tcctctaact acagaaggcc ctacaccatg
     3241 aaaataaaga acattctact caccctttgc acctcacttc tgcttaccaa cgttgcggca
     3301 cacgccaaag aagtcaaaat aggtatggcg attgatgatc tccgtcttga acgctggcaa
     3361 aaagatcgag atatttttgt gaaaaaggca gaatctctcg gcgcgaaagt atttgtacag
     3421 tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg aaaacatgat aaaccggggt
     3481 gttgatgttc ttgtcattat tccgtataac ggtcaggtat taagtaacgt tgtaaaagaa
     3541 gccaaacaag aaggtattaa agtattagct tacgatcgta tgattaacga tgcggatatc
     3601 gatttttata tttctttcga taacgaaaaa gtcggtgaac tgcaggcaaa agccctggtc
     3661 gatattgttc cgcaaggtaa ttacttcctg atgggcggct cgccggtaga taacaacgcc
     3721 aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt acgttgattc cggaaaaatt
     3781 aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg aaaacgcatt gaaaattatg
     3841 gaaaacgcgc taaccgccaa taataacaaa attgatgctg tagttgcctc aaacgatgcc
     3901 accgcaggtg gggcaattca ggcattaagc gcgcaaggtt tatcagggaa agtagcaatt
     3961 tccggccagg atgcggatct cgcaggtatt aaacgtattg ctgccggtac gcaaactatg
     4021 acggtgtata aacctattac gttgttggca aatactgccg cagaaattgc cgttgagttg
     4081 ggcaatggtc aggaaccaaa agcagatacc acactgaata atggcctgaa agatgtgccc
     4141 tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca tcaaagacac ggtagttaaa
     4201 gacggattcc acaaagagag cgagctgtaa gcgttacgcc ccggcgcgga gcgggggcgt
     4261 gatttctctc catgccgcgt gaatgaattg gcttaggtgg agtcgttatg ccttatctac
     4321 ttgaaatgaa gaatattacc aaaaccttcg gcagtgtgaa ggcgattgat aacgtctgct
     4381 tgcggttgaa tgctggcgaa atcgtctcac tttgtgggga aaatgggtct ggtaaatcaa
     4441 cgctgatgaa agtgctgtgt ggtatttatc cccatggctc ctacgaaggc gaaattattt
     4501 ttgcgggaga agagattcag gcgagtcaca tccgcgatac cgaacgcaaa ggtatcgcca
     4561 tcattcacca ggaattggcc ctggtgaaag aattgaccgt gctggaaaat atcttcctgg
     4621 gtaacgaaat aacccacaat ggcattatgg attatgacct gatgacgcta cgctgtcaga
     4681 agctgctcgc acaggtcagt ttatccattt cacctgatac ccgcgttggc gatttagggc
     4741 ttgggcaaca acaactggtt gaaattgcca aggcacttaa taaacaggtg cgcttgttaa
     4801 ttctcgatga accgacagcc tcattaactg agcaggaaac gtcggtttta ctggatatta
     4861 ttcgcgatct acaacagcac ggtatcgcct gtatttatat ttcgcacaaa ctcaacgaag
     4921 tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg acagcacatt ggtacgcgtg
     4981 atgctgccgg aatgagtgaa gacgatatta tcaccatgat ggtcgggcga gagttaaccg
     5041 cgctttaccc taatgaacca cataccaccg gagatgaaat attacgtatt gaacatctga
     5101 cggcatggca tccggtcaat cgtcatatta aacgagttaa tgatgtctcg ttttccctga
     5161 aacgtggcga aatactgggt attgccggac tcgttggtgc cggacgtacc gagaccattc
     5221 agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa aatttatatt gatggcaaac
     5281 aggtagatat tcgtaactgt cagcaagcca tcgcccaggg gattgcgatg gtacccgaag
     5341 acagaaagcg cgacggcatc gttccggtaa tggcggttgg taaaaatatt accctcgccg
     5401 cactcaataa atttaccggt ggcattagcc agcttgatga cgcggaagag caaaaatgta
     5461 ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc ccccgatctt gctattggac
     5521 gtttgagcgg cggcaatcag caaaaagcga tcctcgctcg ctgtctgtta cttaacccgc
     5581 gcatcctcat tcttgatgaa cctaccaggg gtatcgatat tggcgcgaaa tacgagatct
     5641 acaaattaat taaccaactc gtccagcagg gtattgccgt tattgtcatc tcttccgaat
     5701 tacctgaagt gctcggcctt agcgatcggg tactggtgat gcatgaaggg aaactaaaag
     5761 ccaacctgat aaatcataac ctgactcagg agcaggtgat ggaagccgca ttgaggagcg
     5821 aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg ccgtaccgac atccggtggc
     5881 ttttccgggc tgaaatcact gaatttgcag gtcttcgtga tgattgcagc tatcatcgca
     5941 atcatgctgt tctttacctg gaccaccgat ggtgcctact taagcgcccg taacgtctcc
     6001 aacctgttac gccagaccgc gattaccggc atcctcgcgg taggaatggt gttcgtcata
     6061 atttctgctg aaatcgacct ttccgtcggc tcaatgatgg ggctattagg tggcgtcgcg
     6121 gcgatttgtg acgtctggtt aggctggcct ttgccactta ccatcattgt gacgttggtt
     6181 ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg cgtaccgcaa agtcccttca
     6241 tttattgtca ccctcgcggg catgttggca tttcgcggca tactcattgg catcaccaac
     6301 ggcacgactg tttcccccac cagcgccgcg atgtcacaaa ttgggcaaag ctatctacct
     6361 gccagcactg gcttcatcat tggcgcgctt ggcttaatgg cttttgttgg ttggcaatgg
     6421 cgcggaagaa tgcgccgtca ggctttgggt ttgcagtctc cggcctctac cgcagtagtc
     6481 ggtcgccagg ctttaaccgc tatcatcgta ttaggcgcaa tctggctgtt gaatgattac
     6541 cgtggcgttc ccactcctgt tctgctgctg acattgctgt tactcggcgg aatgtttatg
     6601 gcaacgcgga cggcatttgg acgacgcatt tatgccatcg gcggcaatct ggaagcagca
     6661 cgtctctccg ggattaacgt tgaacgcacc aaacttgccg tgttcgcgat taacggatta
     6721 atggtagcca tcgccggatt aatccttagt tctcgacttg gcgctggttc accttctgcg
     6781 ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga ttggcggcac cagcctggct
     6841 ggcggtgtgg gaagcgttgc cggagcagta atgggggcat ttatcatggc ttcactggat
     6901 aacggcatga gtatgatgga tgtaccgacc ttctggcagt atatcgttaa aggtgcgatt
     6961 ctgttgctgg cagtatggat ggactccgca accaaacgcc gttcttgatt ttgataaaaa
     7021 ttttctcaaa gccggttacg tattaccggt tttgagtttt tgcatgattc agcaggaaaa
     7081 gaaccatgtt tactaaacgt caccgcatca cattactgtt caatgccaat aaagcctatg
     7141 accggcaggt agtagaaggc gtaggggaat atttacaggc atcacaatca gaatgggata
     7201 ttttcattga agaagatttc cgcgcccgca ttgataaaat caaggactgg ttaggagatg
     7261 gcgtcattgc cgacttcgac gacaaacaga tcgagcaagc gctggctgat gtcgacgtcc
     7321 ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag ttacccaccc gttcattaca
     7381 ttgccaccga taactatgcg ctggttgaaa gcgcattttt gcatttaaaa gagaaaggcg
     7441 ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg caaacgttgg gccactgaac
     7501 gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta tcgcggagtg gtttatcagg
     7561 ggttagaaac cgcgccagag aactggcaac atgcgcaaaa tcggctggca gactggctac
     7621 aaacgctgcc accgcaaacc gggattattg ccgttactga cgcccgggcg cggcatattc
     7681 tgcaagtatg tgaacatcta catattcccg taccggaaaa attatgcgtg attggcatcg
     7741 ataacgaaga actgacccgc tatctgtcgc gtgtcgccct ttcttcggtc gctcagggcg
     7801 cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg attattagat aaagaagaaa
     7861 tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat tgaacggcgc tcaacagatt
     7921 atcgctcgct gaccgatccc gccgttattc aggccatgca ttacattcgt aatcacgcct
     7981 gtaaagggat taaagtggat caggtactgg atgcggtcgg gatctcgcgc tccaatcttg
     8041 agaagcgttt taaagaagag gtgggtgaaa ccatccatgc aatgattcat gccgagaagc
     8101 tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc gatcaatgag atatcgcaaa
     8161 tgtgcggtta tccatcgctg caatatttct actctgtttt taaaaaagca tatgacacaa
     8221 cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt gtagcgatga tgagaattgt
     8281 cggcgtcaca tcagggaact cagcacagcc acctgatgcg agcaaaagcc ttatccgacc
     8341 gacaaatcag ataaggctca aggaaatgca attacatatg cgccgcgatt aaccgttggt
     8401 tatcctggta cattgcgaac aggtagttgt tataactctt ccctttggtc gagtagccct
     8461 tcagcttgtg aatcatcgct gtggcagtca cttcctgatc cgctttacgc agctgcgcac
     8521 gcgatttacg gaatgaagag taagccggat gcgtattgag gttagtgaca taggcgctca
     8581 ccgattcttt gacagaacta aactgtgagt accctttcac tttacccggc gcattggtac
     8641 aacgtccttt catgcatttc atgccgaaca gattgttgtt gttgcgcgcc agctttgacg
     8701 ttccccaacc gctttctgct gcagccatcg tcgccaccat actggtgggg ataatgtcta
     8761 cgcgttcaag caaggtattc cacgggattt ttcgcgtatt accagaccac ttcaccttgt
     8821 agcgtttggc gatgtctttc agacgcgcac gctcagcagg tgaccattgg ccctgatact
     8881 gttttgaaat cagccagtta cgttccgcag taatggctgc attttggctg gtaatgtaag
     8941 gcattacggt ccggagaaac gcctttttcc ttggtgttcc ggaagggtat tttcgcaaat
     9001 caggaagtga attactcttt gcactattgc gagaatactc ttgtttactg cttacctgtt
     9061 tattactagc tttagttaag tgggactttt gactcgctgt tgttgtgtgc gtcttcgcta
     9121 acacctcact cgaaaacacc agagtgagta acataagaat catcgcccca tatcgtcgta
     9181 tgggagtcaa aatcatcagg tctcctggtc ggatttaatc attccaacac cttatatttt
     9241 tcacaaattt gagagttgaa tctcaaatca tatcaaaaat agctgtcaag agcaccccaa
     9301 ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat tcagattggc gctaaatcgc
     9361 agaaaatgtg gggtttatcg caaaattcag ccgttttttg cgcgagatcg ctcacccttg
     9421 cttctcatcc tgtggactta ccgctcaggg atgagttttg tttggcttat cgctggcaaa
     9481 ctgtctgaaa tcgcagcaat aaggactcat ccgccatgaa actcgccgcc tgttttctga
     9541 cactccttcc tggcttcgcc gttgccgcca gctggacttc tccggggttc cctgccttta
     9601 gtgaacaggg aacggaaaca tttgtcagcc acgcgcagtt gcccaaaggt acgcgtccac
     9661 taacgctaaa ttttgaccaa cagtgctggc agcctgcgga tgcgataaaa ctcaatcaga
     9721 tgctttccct gcaaccttgt agcaacacgc agcctcaatg gcgattgttc agggacggcg
     9781 aatatacgct gcaaatagac acccgctccg gcacgccaac attgatgatt tccatccaga
     9841 acgccgccga accggtagca agccaggtcc gtgtatgccc gaaatgggat ggattaccgc
     9901 tcacgctgga tgtcagcgcc actttcccgg aaggagccgc cgtacgggat tattacagcc
     9961 agcaaattgc gatagtgaag aacggtcaaa taacgttaca acccgctgcc accagcaacg
    10021 gtttactcct gctggaacgg gcagaaactg acgcctctgc ccctttcgac tggcataacg
    10081 ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg cgatcccagt aatgaccaga
    10141 gttacggacg tcataaagac ggtatggcgg aaattggcac ttttcacggc ggcgatttac
    10201 gcggcctgac caacaaactg gattacctcc agcagttggg cgttaatgct ttatggataa
    10261 gcgccccatt tgagcaaatt cacggctggg tcggcggcgg tacaaaaggc gatttcccgc
    10321 attatgccta ccacggttat tacacacagg actggacgaa tcttgatgcc aatatgggca
    10381 acgaagccga tctacggacg ctggttgata gcgcacatca gcgcggtatt cgtattctct
    10441 ttgatgtcgt gatgaaccac accggctatg ccacgctggc ggatatgcag gagtatcagt
    10501 ttggcgcgtt atatctttct ggtgacgagg tgaaaaaaac gctgggtgaa cgctggagcg
    10561 actggaaacc tgccgccggg caaacctggc atagctttaa cgattacatt aatttcagcg
    10621 acaaaacagg ctgggataaa tggtggggaa aaaactggat ccgtaccgat atcggcgatt
    10681 acgacaatcc tggattcgac gatctcacca tgtcgctagc ctttttgccg gatatcaaaa
    10741 ccgaatcaac taccgcttct ggtctgccgg tgttctataa aaacaaaacg gatacccacg
    10801 ctaaagccat cgacggcttt acccctcgcg attacttaac ccactggtta agtcagtggg
    10861 tccgcgacta tgggattgat ggttttcggg tcgataccgc caaacatgtt gagttgcccg
    10921 cctggcagca actgaaaacc gaagccagcg ccgcgcttcg cgaatggaaa aaagctaacc
    10981 ccgacaaagc attagatgac aaacctttct ggatgaccgg tgaagcctgg ggccacggcg
    11041 tgatgcaaag tgactactat cgccacggct tcgatgcgat gatcaatttc gattatcagg
    11101 agcaggcggc gaaagctgtc gattgtattg cgcagatgga tatgacctgg cagcaaatgg
    11161 cggagaaatt gcagggtttc aacgtgttga gctacctctc gtcgcatgat acccgtctgt
    11221 tccgtgaagg gggcgacaaa gcagcagagt tattactatt agcgccaggc gcggtacaaa
    11281 tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac aggttctgat ccgctgcaag
    11341 gtacacgttc ggatatgaac tggcaggatg ttagcggtaa atctgccgcc aacgtcgcgc
    11401 actggcagaa aatcagccag ttccgcgccc gccatcccgc aattggcgcg ggcaaacaaa
    11461 cgacactttc gctgaagcag ggctacggct ttgttcgtga gcatggcgac gataaagtgc
    11521 tggtcatctg ggctgggcaa cagtgacttt tccggcttcc cgttcgtcag tacctcggga
    11581 agccgccaac caggataaaa tgtcagccct aatcagcgtt gcaggataaa gcaccgctca
    11641 ctcttcaaca gaccgatttg caccccagca aatgtagcgt tattgttacc ttccttgcta
    11701 cagagttcga cagatatccc gctatgacat tctccctttt tggtgacaaa tttacccgcc
    11761 actccggcat tacgctgctg atggaagatc tgaacgacgg tttacgcacc cctggcgcga
    11821 ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat gcaggactac ttccagacgc
    11881 tactgaccga catgctggaa agtggcaaag cgactgatgc actgtgcaat tacgacggtc
    11941 cacaggggaa aacggagcta ctcacactgc ttgccggaat gctgcgcgag aagttgggtt
    12001 gggatatcga accacagaat attgcactaa caaacggcag ccagagcgcg tttttctact
    12061 tatttaatct gtttgccgga cgccgtgccg atggtcgggt caaaaaagtg ctgttcccgc
    12121 tggcaccgga atacattggc tacgctgacg ccggactgga agaagatctg tttgtctctg
    12181 cgcgtccgaa tattgaactg ctgccggaag gccagtttaa ataccacgtc gattttgagc
    12241 atctgcatat tggcgaagaa accgggatga tttgcgtctc ccggccgacg aatccaacag
    12301 gcaatgtgat tactgacgaa gagttgctga agcttgacgc gctggcgaat caacacggca
    12361 ttccgctggt gattgataac gcttatggcg tcccgttccc gggtatcatc ttcagtgaag
    12421 cgcgcccgct atggaatccg aatatcgtgc tgtgcatgag tctttccaag ctggggctgc
    12481 ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat catcaccgcc atcaccaata
    12541 tgaacggcat tatcagcctg gcacctggcg gtattggtcc ggcgatgatg tgtgaaatga
    12601 ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat caaaccgttt tactaccagc
    12661 gtgttcagga aactatcgcc atcattcgcc gctatttacc ggaaaatcgc tgcctgattc
    12721 ataaaccgga aggagccatt ttcctctggc tatggtttaa ggatttgccc attacgaccg
    12781 agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat ggtgccgggg cacaacttct
    12841 tcccagggct ggataaaccg tggccgcata cgcatcaatg tatgcgcatg aactacgtac
    12901 cagagccgga gaaaattgag gcgggggtga agattctggc ggaagagata gaaagagcct
    12961 gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt atcaggccta catgatttct
    13021 gcaatatatt gaatttggaa gaatttgtag gccggataag gcgtttacgc cgcatctggc
    13081 ataaacaaag cgcactattc cggcgttgag aaacgccgga aaaacgtttc aaacaggctg
    13141 ctgccgtagc cgatgccgct gcactttctc gacatccatg cactgcaacg cctttgtcgg
    13201 gcaggcttca acacaggccg gtcccgtctc ccgatgccag cacagatcgc acttaatcgc
    13261 cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag ccagcataca
    13321 gcttttacag ccaatgcaac gtgtttgttc aacgaaaata tgcccatgtt cgcggcttat
    13381 cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac aggctactgc
    13441 cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt ctggtgacaa
    13501 cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac aggtacggca
    13561 accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcactc ctttgcacta
    13621 aaacagcaga gtgccagaga gcgcgtccct actgctttga tccaacgaag gaaatgttaa
    13681 agttttggca cttgtccaaa gcgttatgtg atcgcgccca ggtcatccct gacagtaaat
    13741 cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag atttttctct
    13801 cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg cacccgccca
    13861 tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc ttctctgtcc
    13921 atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga acatcgcggg cagctcggta
    13981 atggtattgc gggttaacgg ctggatctca tgctgatggt tttcccagta tgacttcacg
    14041 tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta gagcggcata
    14101 tgctggccaa tataggcacg ggttcgcagc atcccggttg tgggttccag cttataaatc
    14161 aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt ggcgatgttc
    14221 agtgcctcaa gatgcggagc ggcgatatga atgatattca gcgaagacag cgccttctgc
    14281 ccgacggcaa taaatttggt agtcaggcgg taactccctg cggcgggcgc ggtggtcaca
    14341 tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt taaaccagcc
    14401 agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat ctcaatcagc
    14461 atcaacccgc gaaacagact ctggcttccg gctggacgct ctttttcctg cgccatctcg
    14521 ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg tagcgcaaag
    14581 tgtgccgtag ttcacgatct cgacagataa tttataacca cttgattttt atgtcttttg
    14641 aaattcatca atcagattgc tttgttaaaa agtgatcgat atatttgaaa tcaagtttcg
    14701 catattgaaa ttttaagcca aaaaagcgat caaaaaaaca aggaagcctg ggatgaaagt
    14761 gacatttgag cagttaaaag cagcctttaa tcgggtctta atttcacgcg gcgttgacag
    14821 cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc accgaatccg gcgtttattc
    14881 tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg atatcattcc
    14941 tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg atgcccagcg
    15001 ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc gccattgaac tggctgccga
    15061 tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac tggatgcgcg gcggcagcta
    15121 cggctggcag gcggcggaaa aaggctatat tggcatttgc tggaccaact ccatcgccgt
    15181 aatgccgccg tggggcgcaa aagagtgtcg cattggcact aacccgctga tcgtcgccat
    15241 tccttccacg ccgatcacca tggtcgatat gtcgatgtcg atgttctctt acggcatgtt
    15301 agaagttaac cgcctggcgg gccgtcagct cccggtcgat ggtggctttg atgatgaggg
    15361 caatttgacc aaagaacctg gcgttatcga gaagaatcgc cgcattttgc cgatgggcta
    15421 ctggaaaggt tctggcatgt cgattgtgct ggatatgatc gctactctcc tttccgacgg
    15481 cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa tacggcattt cacaaatttt
    15541 tattgccatt gaagtggaca agcttatcga cggtcccacc cgcgatgcca agctgcaacg
    15601 tatcatggat tacgttacta ccgctgaacg cgctgacgaa aaccaggcca tccgcttacc
    15661 cggccacgaa tttactaccc tgctggccga aaaccgccgt aacggtatta ccgttgatga
    15721 cagcgtgtgg gccaaaatcc aggcgttata aggagataag tcatgatttt tggacatatc
    15781 gcgcagccca atccgtgccg tttgcccgcc gccattgaaa aggcgctcga ttttctgcgc
    15841 gctaccgatt tcaacgccct ggagccgggc gttgtcgaaa tcgacggcaa aaatatttat
    15901 gcgcaaatta tcgatttaac cacacgcgaa gcggtggaaa accgtccgga agtgcatcgt
    15961 cggtatatcg atattcagtt tctggcgtgg ggtgaagaga aaatcggcat tgctattgat
    16021 acgggaaata ataaagtcag cgaatcacta ttagagcagc gcgatattat tttttatcat
    16081 gacagtgaac atgaatcatt tatagaaatg ataccgggca gctacgcaat tttctttccg
    16141 caggatgttc accgaccagg ttgtattttg caaacagcct ctgagatacg aaaaattgtg
    16201 gttaaagtcg cattaacggc gctgaattaa tcgaataatc gtctacgcca gaacgactgg
    16261 gtaatgttat tgctctaact atattaataa tttaggttgc tgcacatgca acttgaatta
    16321 tgacagacat agcctcaagg aatagctatg aaaaaaatac ttgaagcaat actggcgatt
    16381 aatctcgccg tactttcctg tattgtgttt ataaacatta ttttaagata tggttttcag
    16441 acaagcattt tatctgtaga tgaattgtca cgttatttat ttgtctggtt aacatttatt
    16501 ggcgcgattg tggcttttat ggataacgcc cacgttcagg tcacctttct ggtggagaaa
    16561 ctctcccccg catggcaacg acgagtcgct ctggttaccc attccttaat cttatttatt
    16621 tgtggcgcac tggcctgggg cgcaacgcta aaaacaattc aggactggag cgattattca
    16681 ccgatcctcg gtttacccat cggcctgatg tatgccgctt gtctgcccac cagcctcgtt
    16741 atcgcattct ttgaactgcg tcatttatat caactcatca cgcgtagcaa ttctctaacg
    16801 tcgccaccgc aaggagctta atcatggctg tgctgatttt tctgggctgt ctgttgggtg
    16861 gtattgctat cggtttgcct attgcctggg cactgttgtt gtgcggggcg gcgttaatgt
    16921 tctggctgga catgtttgat gtccagatca tggcacaaac gctggtgaac ggagccgata
    16981 gcttctccct gctggcgatt ccgttctttg tgctggcggg tgaaatcatg aatgcgggcg
    17041 gcttgtcaaa acgcattgtt gacctgccga tgaaactggt ggggcataaa ccaggcgggc
    17101 tgggctacgt cggcgtgctg gcagctatga ttatggcgag cctttctggt tctgccgttg
    17161 cggataccgc cgccgttgcc gctctactgg tgccgatgat gcgcagcgcc aactacccgg
    17221 tcaaccgggc ggcggggctg attgcctctg gcggcattat cgcgccaatt attccccctt
    17281 ccattccgtt tattatcttc ggcgtttcca gcggattatc catcagtaag ctgtttatgg
    17341 cgggcattgc ccctggcatg atgatgggcg caacgctgat gcttacctgg tggtggcagg
    17401 ccagtcgcct taatttaccc cgccagcaaa aagcaacgat gcaggaaatc tggcactctt
    17461 ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat tattggcggg tttcgctccg
    17521 gtctgtttac ccctaccgaa gccggggccg ttgctgcttt ctatgcgctg tttgtcgcca
    17581 cagttattta ccgtgaaatg accttcgcca cactttggca tgtactcatt ggcgcggcga
    17641 aaaccacctc agtggttatg tttctggtgg cctcggcaca agtttccgcc tggctgatta
    17701 ccattgctga actgccgatg atggtttctg atttactgca accgttggtc gattcaccgc
    17761 gtctgctgtt tatcgtcatt atggtggcta tcctgattgt cggcatggtc atggatttaa
    17821 cgccgacggt attaattctt accccggtat taatgccttt agttaaagag gcaggaatcg
    17881 atccgattta tttcggtgtc atgtttatca ttaactgctc aatcggctta attacaccgc
    17941 ctatcggtaa tgtacttaac gttatttcag gggtggcaaa actcaaattc gatgatgcag
    18001 tcagaggcgt tttcccttac gtcctggttt tatattcatt attagtcgta tttgttttta
    18061 ttcccgatct catcatcctc cctttgaaat ggattaatta aaaggaaaat attatgaaat
    18121 tacgctctgt aacctacgca ttattcattg ctggcctggc tgcattcagc acatcttctc
    18181 tggcggcgca atctttacgt ttcggttatg aaacctcaca aaccgactcg caacatattg
    18241 cggcgaaaaa attcaatgat ttattgcagg agagaaccaa aggcgagctg aaattaaaat
    18301 tgtttccgga tagcaccctc ggtaacgcgc aggcgatgat cagcggcgta cgtggcggca
    18361 ccatcgatat ggaaatgtcc ggctcgaata actttgccgg attatcacca gtgatgaact
    18421 tgcttgatgt ccctttcctg ttccgcgata ccgctcacgc gcataaaacg ctcgacggca
    18481 aagtcggtga tgatctgaaa gcctcacttg aaggtaaagg actaaaagta ctggcctact
    18541 gggaaaacgg ctggcgcgat gtcaccaact cgcgcgcacc ggttaaaacc cccgccgacc
    18601 tgaaagggct gaaaattcgc accaacaata gcccgatgaa tatcgccgca ttcaaagtct
    18661 ttggcgctaa cccgatcccg atgccgtttg ccgaagtcta taccgggctg gaaacccgca
    18721 ctatcgacgc tcaggaacac ccgatcaacg tcgtctggtc agcaaaattt ttcgaagtgc
    18781 agaagtacct ttctctgacg caccacgcct attccccgct tctggtggtg atcaacaaag
    18841 cgaagtttga tggcttaagc ccggagttcc agcaagcgct aatttcatct gcgcaggaag
    18901 cgggtaacta ccagcgcaaa ctggttgccg aagatcagca aaaaatcatc gacggcatga
    18961 aagaagcggg cgtggaagtc atcaccgatc tcgaccgcaa agcctttagc gacgcactgg
    19021 gtactcaggt ccgcgacatg tttgtaaaag atgttccgca gggtgctgat ctactgaaag
    19081 ccgtggatga ggtgcaataa accatgacgc aatactggct ggggttagat tgtggcggta
    19141 gctggctgaa agccgggctg tatgaccgcg aaggccggga ggcaggcgtg cagcgcctgc
    19201 cgctgtgcgc attaagcccg cagccaggtt gggcagagcg cgatatggca gaactgtggc
    19261 aatgctgcat ggctgtcatt cgcgaactgc ttacccattc cggcgttagc ggggaacaaa
    19321 ttgtcggtat cggtatctcc gcacaaggaa aaggcttgtt tttgctggat aaaaacaaca
    19381 agccgctcgg taacgccatt ttatcctcgg atcgccgggc gatggaaatc gttcgtcgct
    19441 ggcaggaaga tggcatcccg gaaaaactct acccgctgac ccgacaaacc ttgtggaccg
    19501 ggcatccggt atcgctgtta cgttggctta aagagcacga accggaacgc tacgcgcaaa
    19561 ttggctgcgt gatgatgacg cacgactacc tgcgctggtg tttaaccggc gtcaaaggct
    19621 gtgaagagag caatatttcc gagtccaatc tctacaacat gagtcttggg gaatatgacc
    19681 cgtgcctcac cgactggctg gggattgctg aaatcaatca caccctgccg cctgttgtcg
    19741 gatctgccga aatctgcggg gagatcaccg ctcagacagc cgtactgacc ggtctgaaag
    19801 cgggtacgcc cgttgtcggc ggcctgtttg atgtggtttc caccgcactc tgcgccggga
    19861 tcgaagacga atttaccctt aatgcggtga tgggcacctg ggcggtgacc agcggcataa
    19921 cccgcggttt acgtgacggt gaagcgcatc cgtatgtcta tggtcgctac gttaacgatg
    19981 gtgaatttat cgttcacgaa gccagcccca cctcttccgg caacctcgaa tggtttaccg
    20041 cacagtgggg agaaatctcg tttgatgaga tcaaccaggc cgttgccagc ttgccgaagg
    20101 ccggtggcga tctctttttc ctgccgttcc tgtacggcag caacgccgga ctcgagatga
    20161 ccagcggttt ctacgggatg caggccattc acacccgcgc acacctgttg caggccattt
    20221 atgaaggggt ggtgttcagc catatgaccc atctcaaccg gatgcgcgaa cgttttactg
    20281 atgtgcacac cctgcgcgtc actggcggcc cggcgcattc cgatgtctgg atgcaaatgc
    20341 tggcggacgt cagcggtctg cgtattgagc tgccgcaggt ggaagaaacc ggctgctttg
    20401 gtgcagcact tgccgcccgc gtcggcaccg gggtttatcg cgatttcagc gaagcccagc
    20461 gtgatttaca gcacccggtg cgcaccctgc tgccggatat gactgtccat cagctttacc
    20521 aacaaaaata ccaacgctat cagcatctca ttgccgcact tcagggcttt cacgcccgca
    20581 tcaaggagca catattatga gccgaccact tctgcaactg gcactcgacc actcatcact
    20641 tgaagccgca cagcgcgacg tgacgcagtt aaaagacagc gtcgatattg tcgaagcggg
    20701 caccattctc tgtttaaacg aagggcttgg cgcggtgaaa gccttgcgcg aacagtgccc
    20761 tgacaaaatc atcgttgccg actggaaggt cgccgacgct ggtgaaacgc tcgcgcaaca
    20821 ggcatttggc gcaggcgcta actggatgac catcatctgc gccgcaccgc tcgcgacggt
    20881 ggaaaaaggc cacgcgatgg cgcaacgctg cggtggtgaa attcagatcg agctgttcgg
    20941 taactggacg ctggacgacg cccgcgactg gcatcgtatt ggcgtgcggc aggcgattta
    21001 tcatcgcggt cgtgatgcac aggccagcgg gcaacagtgg ggcgaagccg atctggcacg
    21061 catgaaggcg ctttccgata tcggccttga gctttccatt accggcggga ttacccctgc
    21121 tgacctgccg ctgtttaaag atattcgcgt gaaagcgttt attgccgggc gagcactggc
    21181 aggctcagca aatccggcgc aggtggctgg cgatttccat gcgcaaatcg acgctatctg
    21241 gggaggcaag cgtgcgtaat catccgttag ggatttatga aaaggcgctg gcgaaagatc
    21301 tctcctggcc ggagcggctg gtgctggcaa aaagctgcgg ttttgatttt gtcgaaatgt
    21361 cggtggatga aaccgacgaa cggctttcac gtcttgactg gagcaccgca caaaggactt
    21421 cgctggttgc cgcgatgatc gaaacgggtg ttgggatccc gtcgatgtgc ctgtccgcac
    21481 atcgtcgctt tccttttggt agccgtgacg acgcggtacg ccagcgagca cgggagatca
    21541 tgagcaaagc gattcgtctg gcacgcgatc tcggcatacg caccatccag ctggcgggct
    21601 acgacgtcta ttacgaagac cacgatgaag gcacccggca acgttttgct gaagggctgg
    21661 cgtgggcggt cgaacaggcg gcggcatcac aagtcatgct agcggtggag attatggaca
    21721 ccgcgtttat gaactccatc agtaaatgga aaaagtggga cgagatgctc gcctcgccgt
    21781 ggttcaccgt ctacccggac gtcggcaacc tcagcgcctg gggcaatgat gttcccgccg
    21841 aactgaaact gggcattgac cgtatcgccg cgatccacct gaaagacacc cagccagtta
    21901 ccgagcaaag tcccggacag ttccgcgacg tgccgtttgg cgaaggctgc gtcgatttcg
    21961 ttggcatctt caaaacgctg cataaactga actatcgcgg ttctttcctg attgagatgt
    22021 ggaccgaaaa agccaaagag ccggtgctgg agattattca ggcgcggcgt tggattgaag
    22081 cgcgtatgca ggaggctgga tttatatgtt agagcaactg aaagccgacg tgctggcggc
    22141 gaatctggcg cttcccgctc accatctggt gacgttcacc tggggcaatg tcagcgcggt
    22201 agacgaaacg cggcaatgga tggtaatcaa accttccggc gtcgagtatg acgtgatgac
    22261 cgccgacgat atggtggtgg ttgagatagc cagcggcaag gtggtggaag gtagcaaaaa
    22321 gccctcgtcc gacacgccaa cgcatctggc gctttaccgt cgctacgccg aaatcggtgg
    22381 cattgtgcat acccactcac gccacgccac catctggtcg caggccgggc tggatctccc
    22441 cgcctggggc accacgcacg ctgattattt ctatggtgcc atcccctgca cgcgacagat
    22501 gaccgcagag gagattaacg gcgaatatga atatcagacc ggcgaagtga tcattgaaac
    22561 cttcgaagaa cgtggcagga gtccggcaca aatcccggcg gtgctggtgc attctcacgg
    22621 cccgttcgca tggggtaaaa acgccgccga tgccgtgcat aacgccgtag tactcgaaga
    22681 atgcgcctat atgggcctat tctcgcgcca gcttgcgccg cagctccctg cgatgcaaaa
    22741 cgaactgctg gataagcact acctgcgtaa gcatggggcc aatgcctatt acgggcagta
    22801 atccctcacg ccggggcttc atcgccccgg cactacgaat tgatatgttc cttgctgtaa
    22861 cgccgcttcc acgctgctgg cgttaaaccg gtgtgcttgc gaaaaatctg ccggaaatag
    22921 ccaacgtcat taaaccgtat tgcatggagc aggccgacaa cgtcgagtca ctgcataacc
    22981 tgttgctgcc cgccgatgcg ccatttacga catttgaagg caagggattg ttcagccata
    23041 agatcctgat tcagacgcca ggttcccggt atgaacagag caatccagag caacaacctt
    23101 tgtggcatta cgacggagca ccagccgcac cgacatccac cggtgaatta aaaacgccag
    23161 ctcacgccgg tgataaaggt aaaactctca ttacgatcaa tcatcgggct gttggtaatc
    23221 tcttccggca gctcgctgta tcctgcgctc gccatcaata cgacgtgctc tcctatcggg
    23281 tattttgccg tcaggctgac atagggcacc caggcatcct gcgcggaata acttgccaga
    23341 ccgctacgac gggactcact ctctgaaatg ccatagtaat agtcactgaa attctcgtca
    23401 taatagagaa cgcccagcgc aggtgtcagc gacaggcgac ctatctgcat tttgtggaat
    23461 accgatagct cccccaccca gccgttgctg ttatccagaa cgtccgcagc cgcagaggct
    23521 ttcacggttc cccaacgctc gtggtgatac caggcaaccc ccgccatagc cgtactatca
    23581 cgcttatcaa gctgttgcat ggcataatcg tcattatcgg cagggtcaaa ttccagcggc
    23641 atccaggatg cggtcaggct aagttcattt ttttcacttt gcgacagaat aaaacctaat
    23701 gtggtctgac gaacataaaa agaatcacct tcataactaa tcagcggaat tgcctttgta
    23761 ttttcattat aaccgcgata aggcgattca ttataagctg caccagcacc aatggataat
    23821 tcagaagcag ttgcgcttgc cataaaaggc aacgcaaata acgccacaat attgcgatta
    23881 attaacatgt ccggtattcc attttaaaat aagataaaaa agagtcggca taataatgct
    23941 t
//
LOCUS       DAZFDP010000066        23235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFDP010000066 DAZFDP010000000
VERSION     DAZFDP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..1507
                     /locus_tag="ACHJ1G_002652"
     CDS             143..1507
                     /locus_tag="ACHJ1G_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764486.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1635..2126)
                     /gene="yajQ"
                     /locus_tag="ACHJ1G_002653"
     CDS             complement(1635..2126)
                     /gene="yajQ"
                     /locus_tag="ACHJ1G_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4764487.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2294..3205
                     /gene="panE"
                     /locus_tag="ACHJ1G_002654"
     CDS             2294..3205
                     /gene="panE"
                     /locus_tag="ACHJ1G_002654"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4764488.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3168..3758
                     /gene="yajL"
                     /locus_tag="ACHJ1G_002655"
     CDS             3168..3758
                     /gene="yajL"
                     /locus_tag="ACHJ1G_002655"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4764489.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3812..5260)
                     /gene="thiI"
                     /locus_tag="ACHJ1G_002656"
     CDS             complement(3812..5260)
                     /gene="thiI"
                     /locus_tag="ACHJ1G_002656"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4764490.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5466..5708
                     /gene="xseB"
                     /locus_tag="ACHJ1G_002657"
     CDS             5466..5708
                     /gene="xseB"
                     /locus_tag="ACHJ1G_002657"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4764491.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5708..6607
                     /gene="ispA"
                     /locus_tag="ACHJ1G_002658"
     CDS             5708..6607
                     /gene="ispA"
                     /locus_tag="ACHJ1G_002658"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4764492.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6632..8494
                     /gene="dxs"
                     /locus_tag="ACHJ1G_002659"
     CDS             6632..8494
                     /gene="dxs"
                     /locus_tag="ACHJ1G_002659"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4764493.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8674..9648
                     /gene="yajO"
                     /locus_tag="ACHJ1G_002660"
     CDS             8674..9648
                     /gene="yajO"
                     /locus_tag="ACHJ1G_002660"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4764494.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9702..10220)
                     /gene="pgpA"
                     /locus_tag="ACHJ1G_002661"
     CDS             complement(9702..10220)
                     /gene="pgpA"
                     /locus_tag="ACHJ1G_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4764495.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10198..11175)
                     /gene="thiL"
                     /locus_tag="ACHJ1G_002662"
     CDS             complement(10198..11175)
                     /gene="thiL"
                     /locus_tag="ACHJ1G_002662"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4764496.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11253..11672)
                     /gene="nusB"
                     /locus_tag="ACHJ1G_002663"
     CDS             complement(11253..11672)
                     /gene="nusB"
                     /locus_tag="ACHJ1G_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4764497.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11692..12162)
                     /gene="ribE"
                     /locus_tag="ACHJ1G_002664"
     CDS             complement(11692..12162)
                     /gene="ribE"
                     /locus_tag="ACHJ1G_002664"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4764498.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12251..13354)
                     /gene="ribD"
                     /locus_tag="ACHJ1G_002665"
     CDS             complement(12251..13354)
                     /gene="ribD"
                     /locus_tag="ACHJ1G_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4764499.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13358..13807)
                     /gene="nrdR"
                     /locus_tag="ACHJ1G_002666"
     CDS             complement(13358..13807)
                     /gene="nrdR"
                     /locus_tag="ACHJ1G_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4764500.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13958..14497
                     /locus_tag="ACHJ1G_002667"
     CDS             13958..14497
                     /locus_tag="ACHJ1G_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4764501.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14796..15680
                     /gene="tsx"
                     /locus_tag="ACHJ1G_002668"
     CDS             14796..15680
                     /gene="tsx"
                     /locus_tag="ACHJ1G_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4764502.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15857..16204)
                     /gene="yajD"
                     /locus_tag="ACHJ1G_002669"
     CDS             complement(15857..16204)
                     /gene="yajD"
                     /locus_tag="ACHJ1G_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4764503.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16333..17304)
                     /gene="secF"
                     /locus_tag="ACHJ1G_002670"
     CDS             complement(16333..17304)
                     /gene="secF"
                     /locus_tag="ACHJ1G_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4764504.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17315..19162)
                     /gene="secD"
                     /locus_tag="ACHJ1G_002671"
     CDS             complement(17315..19162)
                     /gene="secD"
                     /locus_tag="ACHJ1G_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4764505.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19190..19522)
                     /gene="yajC"
                     /locus_tag="ACHJ1G_002672"
     CDS             complement(19190..19522)
                     /gene="yajC"
                     /locus_tag="ACHJ1G_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4764506.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19545..20672)
                     /gene="tgt"
                     /locus_tag="ACHJ1G_002673"
     CDS             complement(19545..20672)
                     /gene="tgt"
                     /locus_tag="ACHJ1G_002673"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4764507.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20728..21798)
                     /gene="queA"
                     /locus_tag="ACHJ1G_002674"
     CDS             complement(20728..21798)
                     /gene="queA"
                     /locus_tag="ACHJ1G_002674"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4764508.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21891..22472
                     /gene="acpH"
                     /locus_tag="ACHJ1G_002675"
     CDS             21891..22472
                     /gene="acpH"
                     /locus_tag="ACHJ1G_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4764509.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22477..>23235)
                     /locus_tag="ACHJ1G_002676"
     CDS             complement(22477..>23235)
                     /locus_tag="ACHJ1G_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO4764510.1"
                     /translation="HVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGF
                     TFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKT
                     LLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFA
                     LYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLPAS
                     PLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5634 a         6212 c         5912 g         5477 t
ORIGIN      
        1 tatagagcat tcattttatg aatgcaaaaa aacatacctg acacacaaat tactctcctc
       61 tgtaatattt tctcattaac actctgttta cccaacatgc gctccgcact acactatgtg
      121 ggcatttttt taaggggtgg taatgaacga ttataaaatg acgccaggtg agaggcgcgc
      181 gacctggggt ttagggaccg tattctcgtt gcgcatgctg ggcatgttta tggttctgcc
      241 ggttctgacc acgtacggca tggctctgca aggtgccagc gaagcattaa tcggtattgc
      301 cattggtatt tatggtctga ctcaggccgt ttttcagatt ccgtttggcc tgctttccga
      361 ccgcattggt cgcaaaccat taattgtcgg tgggctggcg gtgtttgccg ccggtagcgt
      421 tatcgctgcg ctttccgact ccatctgggg aattattctg ggccgggcgc tacaaggctc
      481 tggagcaatt gccgccgccg ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg
      541 caccaaagcg atggcgttta tcggcgtgag ctttggcatt acctttgcca ttgcgatggt
      601 gcttggcccg atcatcactc acaaacttgg gctgcacgcg ctgttctgga tgatcgctat
      661 tctggcaaca accggcattg cgttgaccat ttgggttgta cccaacagta gcactcacgt
      721 acttaatcgt gagtccggaa tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg
      781 gctgctaaaa ctcaactttg gcattatgtg tctgcacatg cttctgatgt ctacgtttgt
      841 tgccctgccc ggacagctag ctgatgcggg gttcccggcg gctgaacact ggaaggtcta
      901 tctggcgacg atgttaatcg cctttggctc ggtcgtgcct ttcattatct acgctgaagt
      961 taagcgcaaa atgaagcaag tctttgtctt ctgcgtcggg ttgatcgtgg ttgcggaaat
     1021 tgtgttgtgg aacgcacaaa cgcagttctg gcaactggtg gtcggcgtgc agcttttctt
     1081 tgtggcgttt aatttgatgg aagcccttct gccctcactt atcagtaaag agtcgccagc
     1141 aggttacaaa ggtacggcga tgggtgtcta ctccaccagc cagtttcttg gcgtggcgat
     1201 tggcggttcg ctgggcggct ggattgacgg catgtttgac ggtcaggggg tatttctcgc
     1261 tggcgcaatg ctggccgcag tgtggctggc agtcgccagt accatgaaag aaccgccgta
     1321 tgtcagcagt ttgcgcattg aaatcccggc ggacattgcc gcaaacgaag cgttaaaagt
     1381 gcgtttgctg gaaactgaag gcgtcaaaga agtgttgatt gcagaagaag aacattcagc
     1441 ttatgtgaaa atcgacagca aagtgacgaa tcgctttgag atagaacagg caattcgtca
     1501 ggcataaaaa acgcggcgaa ttgcctgctg cgctacgctt atcaggccta caaagtttct
     1561 gaattacaaa cctttgtagg ccgaatcagg cgttcatgcc gcatctgaca tgaacaaaac
     1621 gcacatagtc gcgattaatc gcggaagttt ttgaactgga acggctgacc gagatcgcca
     1681 ccacgtacca tcgccatgac agcctgcaaa tcatcacgag atttgcccgt tacgcggatt
     1741 tcatcgccct gaatttgcgc ctgcactttc agtttgctgt ctttgatcat cttgacgatt
     1801 ttcttctggg tcgcgctctc aatgccctgt ttcagtttcg cttccacaaa ccaggtttta
     1861 ccgctatgaa cgatattctc cggtacatcc agcgaactgc cttcaatgcc gcgcttcagc
     1921 agcttggcac gcagaatatc cagcaactga ttgacctgga aatcggactc gctcaacact
     1981 ttgatggttt tgctggcgtc gttcagctca aatgaggctt caacgttacg gaagtcaaaa
     2041 cgggactcca cttcgcggct cgcgttatcg accgcgttac gtgcttcctg aagatcaact
     2101 tcagagacaa tatcgaaaga tggcatcttt tcctctccct tcatttttga tgcgaagcat
     2161 aatacccgca aagttaaata accgacagtc ctgcaccagg acgtatccct taattgtgca
     2221 tagtcgcaac agcatgcaca attaagggat agccttattg aacacccgga gtggttgcgg
     2281 gtgaggagga acaatgaaaa ttaccgtatt gggatgcggt gccttagggc aattatggct
     2341 tacagcactt tgcaaacagg gtcatgaagt tcagggctgg ctgcgcgtac cgcaacctta
     2401 ttgtagcgtg aatctggttg agacagatgg ttcgatattt aacgaatcgc tgaccgccaa
     2461 cgatcccgat tttcttgcca ccagcgatct gctcctggtg acgctgaaag catggcaggt
     2521 ttacgatgcc gtcaaaagcc tcgcgtccac actgcctgta accacgccaa tactgttaat
     2581 tcacaacggc atgggcacca tcgaagagtt gcaaaacatt cagcagccat tactgatggg
     2641 caccaccacc catgccgccc gccgcgacgg caatgtcatt attcatgtgg caaacggaat
     2701 cacgcatatt ggtccggcac ggcaacagga tggcgattac agttatctgg cggatatttt
     2761 gcaaaccgtg ttgcctgacg ttgcctggca taacaatatt cgcgccgagc tgtggcgcaa
     2821 gctggccgtc aactgtgtga ttaatccact gaccgccatc tggaattgcc cgaatggtga
     2881 attacgtcat catccgcaag aaattatgca gatatgcgaa gaagtcgcgg cagtgatcga
     2941 gcgcgaaggg catcatactt cagcagaaga tttgcgtgat tacgtgatgc aggtgattga
     3001 tgccacagcg gaaaatatct cgtcgatgtt gcaggatatc cgcgcgctgc gccacaccga
     3061 aatcgactat atcaatggtt ttctcttacg ccgcgcccgc gcgcatggga ttgccgtacc
     3121 ggaaaacacc cgcctgtttg aaatggtaaa aagaaaggag agtgaatatg agcgcatcgg
     3181 cactggtttg cctcgcccct ggtagtgaag agactgaagc cgtcaccact atcgatctgc
     3241 tggttcgcgg cggtatcaaa gtcaccactg ccagcgtcgc cagcgatggc aacctggcca
     3301 ttacctgctc gcgcggcgtg aagctgctgg cggatgcgcc gctggtcgag gtggctgatg
     3361 gcgaatatga cgtgatcgta ctgcctggtg gcattaaagg cgcagagtgt tttcgcgata
     3421 gtaccctgct ggttgaaacc gttaaacagt tccaccgttc cgggcgtatc gtcgcggcta
     3481 tttgcgccgc gccagccacc gtgctggtgc cgcacgatat cttcccgatt ggtaatatga
     3541 ccggcttccc gacgctgaaa gacaaaattc ccgctgaaca atggcaggac aagcgcgtcg
     3601 tctgggatgc acgggtaaaa ttgctgacca gccaggggcc ggggacagct atcgactttg
     3661 gtctgaaaat tatcgacctg ctggttgggc gcgagaaagc ccatgaagtg gcatcacaac
     3721 tggtgatggc ggcagggatt tataattatt acgagtagtg tcggatgcgg taagcgtcgc
     3781 atctgaccag atgcgacgtt acaaccacaa attacgggcg atataccttc acattgttaa
     3841 agccctgctc gcgcagatag agcgcctgca gacggctcat caccccgcgc tcacaccaca
     3901 gtagccaggt tctgttctgg tcgagatcgc caaacttggt gctcagttta tagaacggca
     3961 gagaaactac atcaatccct tcgactttca gtggcttatc ttcctgttca tcgatagaac
     4021 ggatatcgag gatcacgtcg ttcgggccga agccattgac ggtttccact tccaccactt
     4081 cctgctcggt ctgctgggcg atttcgcgga tatcaacgtt attcgcttct tcaaccactt
     4141 tatcgagaat gctgaagtcg aatttctctt cttccgcttc aatcttcgac ttaaccgctt
     4201 tcaccgtcgg gcttttggag ataacaccgc aatattccgg catcgtgcga gcaaagtctt
     4261 cggtgccaat ctggcgagcc aggttgatga tgtgctcttt gtcgtaagaa atcagcggac
     4321 gcaggatcag cgtatcggag acgttatcaa tcaggcgcag gttggtcagc gtctgactgg
     4381 acacctggcc gagcgcttcg ccggtgacca gcgcctgtac gccgtaacgt tcagccactt
     4441 tagacgcggc acgcaccatc atacgtttga ggataacgcc catctgaccg tcgtcgattt
     4501 tctcgagaat ttccccgacg accggttcga aattaatagc gacaaaacgc acgcggtggg
     4561 agctgccaaa acggttccac agataatgcg ccacctgacg cacgccaatt tcatgcgccg
     4621 cgccgccaag gttaaagaaa cagtaatgca cgcggcagcc gcgacgcatc aacatataac
     4681 tggaaacacc ggagtcgaaa ccaccggaaa tgagcgacag cacatcttcc tgggtgccga
     4741 tcgggaaacc gccaatacct tcgtagcggc ctttaatcag caggagacga tcgtcttcca
     4801 cttccagatg gacagtcact tccggattgg tcagcttcac gcgcgcggac tcaatatgct
     4861 gatttaaacc gccgccgacg taacgttcca catcaatcga gctaaaatca tgtttgccac
     4921 ggcgcttcac gcgtacgcag aaggttttgc cttccagctg atcgcgatac tgaaccaacg
     4981 ctttctcaaa aatatcgtgc atgtcggtaa acggcacgtc ttcgacttcg agaatatggt
     5041 ggatacccgg aatacgggtc agggcgtcgc gaatagccag acgctggttt tcatcttttg
     5101 cgcgaacttc gatgttatcc cagtggcgga cgacagcgag cgtctcatca tagtgcttta
     5161 aaacgttacg aatgttcccg gtaaggattt ttataaagcg caagcgcaca gattggcttt
     5221 tgatggtgat ttccgggaac aatttaatga taaacttcat ggcggcaatg gttcgttggc
     5281 aagccttaag cgacttgtat agggaaaaat acagcagccc acacctgcgg ctgcatccag
     5341 gcgcggaagt ataccactaa catcgctttg ctgcgcacat caccttacca ttgcgcgtta
     5401 tttgctattt gccctgagtc cgttaccatg acggggcgaa aaatattgag agtcagacat
     5461 tcattatgcc gaagaaaaat gaggctcccg ccagctttga aaaggcgctg agcgagctgg
     5521 aacagattgt aacccgtctg gaaagtggcg acctgccgct ggaagaggcg ctgaacgagt
     5581 tcgaacgcgg cgtgcagctg gcacgtcagg ggcaggccaa attacaacaa gccgaacagc
     5641 gcgtacaaat tctgctgtct gacaatgaag acgcctctct aacccctttt acaccggaca
     5701 atgagtaatg gactttccgc agcaactcga agcctgcgtt aagcaggcca accaggcgct
     5761 gagccgtttt atcgccccac tgccctttca gaacactccc gtggtcgaaa ccatgcagta
     5821 tggcgcatta ttaggtggta agcgtctgcg acccttcctg gtttatgcca ccggtcatat
     5881 gtttggcgtt agcacaaaca cgctggacgc acccgctgcc gccgtagagt gtatccacgc
     5941 ttactcatta attcatgatg atttaccggc gatggatgat gacgatctgc gtcgcggttt
     6001 gccgacctgc catgtgaagt ttggcgaagc aaacgcgatt ctcgctggcg acgctttaca
     6061 aacgctggcg ttctcgattc taagcgatgc caatatgccg gaagtgtcgg atcgcgacag
     6121 aatttcgatg atttctgaac tggcgagcgc cagcggtatt gccggaatgt gcggtggtca
     6181 ggcattagat ttagacgcgg aaggcaaaca cgtacctctg gacgcgcttg agcgtattca
     6241 tcgtcataaa accggcgcat tgattcgcgc cgccgttcgc cttggtgcat taagcgccgg
     6301 agataaaggg cgtcgtgctc tgccagtact cgacaagtac gcagagagca tcggccttgc
     6361 cttccaggtt caggatgaca tcctggatgt ggtaggagat actgcaacgt tgggaaaacg
     6421 ccagggtgcc gaccagcaac ttggtaaaag tacctaccct gcacttctgg gtcttgagca
     6481 agcccggaag aaagcccggg atctgatcga cgatgcccgt cagtcgctga aacaactggc
     6541 tgaacaatca ctcgatacct cggcactgga agcgctagcg gactacatca tccagcgtaa
     6601 taaataaaca ataagtatta ataggcccct gatgagtttt gatattgcca aatacccgac
     6661 cctggcactg gtcgactcca cccaggagtt acgactgttg ccgaaagaga gtttaccgaa
     6721 actctgcgac gaactgcgcc gctatttact cgacagcgtg agccgttcca gcgggcactt
     6781 cgcctccggg ctgggcacgg tcgaactgac cgtggcgctg cactatgtct acaacacccc
     6841 gtttgaccaa ttgatttggg atgtggggca tcaggcttat ccgcataaaa ttttgaccgg
     6901 acgccgcgac aaaatcggca ccatccgtca gaaaggcggc ctgcacccgt tcccgtggcg
     6961 cggcgaaagc gaatatgacg tattaagcgt cgggcattca tcaacctcca tcagtgccgg
     7021 aattggtatt gcggttgctg ccgagaaaga aggcaaaaat cgccgtaccg tctgtgtaat
     7081 cggcgatggc gcgattaccg caggcatggc gtttgaagcg atgaatcacg cgggcgatat
     7141 ccgtcctgat atgctggtgg tcctcaacga caacgaaatg tcgatttccg aaaatgtcgg
     7201 cgcgctcaac aaccatctgg cgcagctgct ttccggtaag ctttactctt cactgcgcga
     7261 aggcgggaaa aaagttttct ctggcgttcc gccaattaaa gagctgctca aacgtaccga
     7321 agaacatatt aaaggcatgg tagtgcctgg cacgttgttt gaagagctgg gctttaacta
     7381 catcggcccg gttgacggtc acgatgtgct ggggcttatc accacgctga agaacatgcg
     7441 cgacctgaaa ggcccgcagt tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc
     7501 ggcagaaaaa gaccccatca ctttccacgc cgtgcctaaa tttgatccct ccagcggttg
     7561 tttgccgaaa agtagcggcg gtttgccgag ctattcaaaa atctttggcg actggttgtg
     7621 cgaaacggca gcgaaagaca acaagctgat ggcgattact ccggcgatgc gtgaaggttc
     7681 cggcatggtc gagttttcac gtaaattccc ggatcgttac ttcgacgtgg caatcgccga
     7741 gcaacacgcg gtgacctttg ccgccggtct ggcgattggt gggtacaaac ccattgtcgc
     7801 gatttactcc actttcctgc aacgcgccta tgatcaggtg ctgcatgacg tggcgattca
     7861 aaagcttccg gtcctgttcg ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac
     7921 ccatcagggc gcttttgacc tctcttacct gcgctgcata ccggaaatgg tcattatgac
     7981 cccgagcgat gaaaacgaat gtcgccagat gctctatacc ggctatcact ataacgacgg
     8041 cccgtccgcg gtgcgctacc cgcgcggtaa cgcggttggc gtggaactga cgccgctgga
     8101 aaaactgcca attggcaaag gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa
     8161 ctttggtacg ctgatgccag acgcggcgaa agtcgctgaa tcgctgaacg ctacgctggt
     8221 cgatatgcgt tttgtgaaac cgcttgatga agcgttaatt ctggaaatgg ccgccagcca
     8281 tgaagcgctg gtcaccgtag aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa
     8341 cgaagtgcta atggcccatc gtaaaccagt acccgtgctg aacattggcc tgcctgactt
     8401 ctttattcca caaggaactc aggaagaaat gcgcgccgaa ctcggcctcg atgccgccgg
     8461 tatggaagcc aaaatcaagg cctggctggc ataatcccta ctccactcct gctatgctta
     8521 agaaattatt catagactct aaataattcg agttgcagga aggcggcaaa cgagtgaagc
     8581 cccaggagct tacataagta agtgactggg gtgaacgaat gcagccgcag cacatgcaac
     8641 ttgaagtatg acgagtatag cgggagtggc agcatgcaat acaacccctt aggaaaaacc
     8701 gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc agatcgcggt
     8761 aatcacgcat ggacactgcc ggaagagagc agccgtccca tcattaaacg cgcgctggaa
     8821 ggcggcataa atttctttga taccgccaat agctattccg atggcagcag cgaagagatc
     8881 gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tggtcgttgc gacgaaagtg
     8941 ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt gcgctctatc
     9001 gacgacagcc tgcgccgtct cggcatggat tatgtcgata tcctgcaaat tcatcgctgg
     9061 gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt aaaagccggg
     9121 aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca ggccctgaag
     9181 ctacaaaaac agcacggctg ggcgcagttt gtcagtatgc aggatcacta caatctgatt
     9241 tatcgcgaag aagagagcga gatgctgcca ctgtgttatc aggagggcgt ggcggtgatt
     9301 ccgtggagcc cgctggcgcg ggggcgactg acgcgtccgt ggggagaaac taccgcacga
     9361 ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag
     9421 atcgcagagc ggttaacagg cgtcagcgaa gaactcggtg caacacgagc acaagttgcg
     9481 ctggcctggt tgttgagtaa accgggcatt gccgcaccga ttatcggaac ttcgcgcgaa
     9541 gaacagcttg atgagctatt gaacgcggtg gatatcactt tgaagccgga acagattgcc
     9601 gaactggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga gtccaggcct
     9661 gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag aatacccagc
     9721 ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc gacaatatcg
     9781 tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat cggccacggc
     9841 ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg ccagtcattg
     9901 gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac aatgctgccg
     9961 tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc gatacagatc
    10021 cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag ataccagaac
    10081 ggaatcgctg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc acttccgaat
    10141 ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc gacatcttta
    10201 tggcgtggca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc cgtcacgaat
    10261 aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg gtacgcccag
    10321 gtgtccgaga gccacatcca gcgcgccacg gttcagttcc ggcacagtga aacacaactc
    10381 gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa catggcgaga
    10441 aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt
    10501 cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg aatttgccag
    10561 atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac gtttgatcaa
    10621 gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg ccagcccggc
    10681 ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg ccccagagcg
    10741 cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg ataatggccc
    10801 acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat caaacaaact
    10861 gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg ccagcgtcag
    10921 ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcactgcca gtgctttata
    10981 cgccagatca gcaggatcga tatcatggag gaagtggttg cccgccacca gcgtatcagt
    11041 gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt cgccgatgcc
    11101 cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa tcagggagaa
    11161 ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg gcgatcgcgg
    11221 aacggtcttc cgtgaatcta ccggcctgga tatcactttt tgttagggcg aatcacaggt
    11281 gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc gaacgatttc
    11341 gccagttcga tcgcttcgtt aatggccact ttgtatggca catcgctacg tttagacagc
    11401 tcgtacagcg caatgcgcag tactgctttt tctacctgtc ccagttcttc cagcaggcgg
    11461 gacaggtatg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac cccggccagc
    11521 agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag gaactggtat
    11581 tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg gacggcacac
    11641 tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat ttcaggcctt
    11701 gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg cacctttgtt
    11761 gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc
    11821 aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc
    11881 agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg caatcaccgc
    11941 gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat aggcaccagg
    12001 cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca gtgcgtcaat
    12061 tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac
    12121 gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc ttcgggttcg
    12181 agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc ttccctttcg
    12241 agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca tgacgtatct
    12301 ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc gtgcataatc
    12361 cgcgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca tcgactaaac
    12421 ccgcctgcag caatgcgcca gcgagcgttg gccccgcttc cacccagata ctgttaattt
    12481 gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta tgctctggaa
    12541 tcagcaaggt acgcaccgtt tccggccact cactagaatc ttcctgcgta cgcgcgaacc
    12601 aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt tggctatcaa
    12661 tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga gtttgttcat
    12721 ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg gcgctgctag
    12781 ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc tgaggcgaag
    12841 tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat gcgccaagtt
    12901 ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta ttcaattgct
    12961 cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa
    13021 gtccacgccc agcgacctgc gggttaggat cttgcatcga ggcaaccacg cgcgctacgc
    13081 cagcggcgat gagtgcgtca cagcacggtg gcgtacgacc atgatggcta cagggttcga
    13141 gtgtgacata ggcggtcgca cctttggctt tttcacccgc catacgcaac gcgtgtactt
    13201 cggcatgtgg ttcacccgca cgttggtggt aaccttcacc gacaatttcg ccatctttga
    13261 caatgacgca cccgacattc gggttgggat gcgtggtaaa acgtcctcgt tgcgccagct
    13321 ttagcgctcg cgccatgtaa tactcgtcct gcacggctta gtcctccagg cgcgcgatct
    13381 cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa cggatatagg
    13441 cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc ttgctcggca
    13501 cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc atttcgacgt
    13561 catcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc aatttctctt
    13621 cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc agctccgcca
    13681 cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg cgtacggatg
    13741 aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc
    13801 aatgcatacg gcgtcctgac caggtggtta acagaaatct attttaccct gaactgacga
    13861 tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc
    13921 taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact
    13981 gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcagagcg aagtgcgaca
    14041 gatgaaacac agcgtcagca cgctgaacca agagatgacg caactcaata aagaaacggt
    14101 caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt accttctgcc
    14161 aggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt
    14221 gaatattacg cctgatgcag atggcaccac cctgacgcta cgtattcagg gcgagtcgaa
    14281 tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga
    14341 caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc
    14401 ccccagcgat gttgatattc cgttacagtt aaagggtatt tctgtagaac agttagactt
    14461 cgtacgtatc cacgatattc aacctgttat gcagtaaacg tatttcgggg cgattttgtg
    14521 cgtctcgcaa catctttccc cgtcattttg ttactccgct tacatcaccc tgattgatag
    14581 taaaagtttg caacaagggt gaaagtcagt acaatccctg cccgaatgtg tgtaaacgtg
    14641 aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg
    14701 atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc
    14761 actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg
    14821 gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc
    14881 agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt
    14941 tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact
    15001 ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta
    15061 aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg
    15121 acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca
    15181 actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc
    15241 tgggtaccga catcgatact ggtctgccga tgagcctgtc catgaacgtc tatgcgaaat
    15301 accagtggca gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa
    15361 ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca
    15421 ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta
    15481 agacccgtac taataactct atcgcttcca gccatattct ggctctgaac tacgatcact
    15541 ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag
    15601 aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg
    15661 ttgtaggtta caacttctga ttatgaaaat gccgggattt attcccggca tttcagattg
    15721 ttaacaacgt gcgctttgtt catgccggat gcggcgtaaa cgctttgtcc gacccacaaa
    15781 agcacgtaaa ttcaatatat tgctgaaata acgtagtcct gataagcgta gcgcatcagg
    15841 caattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt
    15901 tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat
    15961 actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga caaagcaatt
    16021 cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacagtaa
    16081 gttcacgcag gttggaataa acaaactcgc gggaacagcg accgcagacc cacggatata
    16141 tttttaatgc tttttcgcga tagccgcttt ctaaccgcgc atagtttttt gggatgatag
    16201 ccatgaagag ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat
    16261 tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca
    16321 acgggaactt gattacggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg
    16381 ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg ccacatagat
    16441 ggaagatgca gtaccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac
    16501 cggaccaccg aagaggtaca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa
    16561 ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg
    16621 gatcttgcgg aagttttcac gaatacggtc cgataccacg atactgtcgt taagcgagta
    16681 accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga
    16741 caaaataccc agcgtaatga taacgtcgtg cgccagcgca ataaccaccc ctgccgccag
    16801 tcgccactca aagcgaaaac ctacgtacac aaggatagac agcagcgccg ccatcaacgc
    16861 catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga ccgacgaact caatacgctt
    16921 cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacctggc tgcccagcac
    16981 ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc
    17041 aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc
    17101 aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc
    17161 ccagttaaag ccgcgcacgc ccataataac gatagcggcg attaacagca gaccagagat
    17221 gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg
    17281 ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct
    17341 tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg
    17401 tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt
    17461 acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc
    17521 cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa
    17581 tacgttcgtt gatcagtacg ttcgcatcga ccgccaccgc aagggttaag acgatacccg
    17641 cgatacctgg catactcagc gttgcgcctg gcaacagcga cataatgccg acgattaaga
    17701 tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca
    17761 tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgt tcaatgttct
    17821 gcatacccag ggttgggcca atggtacgtt cttcaacaat ctgaatcggc gcgatcaacg
    17881 caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga
    17941 tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt
    18001 tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca
    18061 gggttgccat cggtttgccg atgttgtcct tagtgaagtt agacatgatg ttaccaccag
    18121 cgctatcgag cgagatgtta acctgcggtt ggttgtattc gtcctggctg gaagtggagt
    18181 cggtgatatg gtcaccggtc agaattacgc gtttgtacag cacaactggc tgaccttcgc
    18241 gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctggtcaa
    18301 cgttggtgtt taccagacgg aattccagcg ttgccgtcgc acccagaatc tctttcgcac
    18361 gcgcagtgtc ctgaatacct ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa
    18421 ccaccggctc cgccacgcca agttggttta cacggttacg caggatattg atgttctgct
    18481 gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt
    18541 tgctgccctg gctactaatc accaggtccg gatggcgctt gctcagatac gcaatggctt
    18601 catcacgagc tttagcatcg cggaaagtaa tgctcaggcc gtagttgttt tctttacgaa
    18661 cggtggtata cgggatgccc ttttcacgca gatcgctgcg caggctatcg atattttgtt
    18721 cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac
    18781 gcaggtcaag gccgagcttc atcggctcag cgtgaatagc tgccagccag cgcggcgttg
    18841 ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac
    18901 gagcgcgcag ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg
    18961 ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt
    19021 gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca
    19081 ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc
    19141 acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct
    19201 tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg
    19261 tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca
    19321 ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt
    19381 ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca
    19441 ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg
    19501 catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa
    19561 ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc
    19621 tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg
    19681 gtgttgagtc gcgcgcctaa tatttcgttg caacggtcaa gatgatgcaa gtaagcgcgt
    19741 gaataattgc gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta
    19801 tacttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg
    19861 ttgcgggttg gcattacgca gtcaaacata tcgataccac gacgtacgcc ttcaaccagg
    19921 tcttctggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcgggcat
    19981 acatgctcca gaatgcggtg catatctgct ttcggctcac ccacagccag accgccgaca
    20041 gcgtagccat caaaaccgat atctaccaga cctttaacag aaatatcacg taaatcttcg
    20101 taaacgctgc cctgaatgat accaaacagc gcatttttgt ttccgagact gtcaaaacgc
    20161 tcacggctac gcttcgccca acgcagagac atctccatgg agcgttttgc gtaatcccag
    20221 tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac
    20281 tgaatctcca ttgatttttc aggatcgagg aaaatcggat cgccgttgat cgggttacgg
    20341 aagtgcacgc cctgttcggt gattttacga atatcgccaa ggctgaagac ctggaagccg
    20401 ccggagtcgg tgaggatcgg ccccttccac tgcataaaat cgtgcagatc gccgtgcagt
    20461 ttcatgattt cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc
    20521 gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca
    20581 ggcataaaac aaggcgtttc cactacgcca caatcaaaga ccaggcggcc acggcgtgcg
    20641 cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt
    20701 ccaacgtttt aaaccagcgc cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg
    20761 attgtacgtg ataaacatcg catcaccgta actaaaaaag cgatatttct cttctaccgc
    20821 tgctttatag gcgttcatgg tgtgttgata accggcaaag gccgaaacca gcataatcag
    20881 cgtcgattcc ggcaggtgga agtttgtcac cagcgcatcg accactttgt actggaagcc
    20941 cggatagata aagatttggg tatcgtcaaa gaacggttcg ataagatcgt ttttcgccgc
    21001 ctgagccgcg ctttccagcg aacgtactga ggtggtgcca accgcaatca cccggttacc
    21061 gcgcgctttc gccgccagta ccgcgtctac cacatcctgc ggcacttcag cgtattccga
    21121 gtgcatgatg tgatcttcaa tggtgtcgac gcgcaccggc tggaaggtgc ccgcaccaac
    21181 gtgcaacgtc acaaacgcca tctccacgcc tttggcgcgt aatttttcca gcaaaggttc
    21241 gtcaaaatgc agacctgcgg tcggggctgc aaccgcgccc ggtttttcgc tataaacggt
    21301 ttgataaagt tcgcggtcag cgtcttcgtc cggacggtcg atatacggcg gcagcggcat
    21361 atggccgatg ctgttgagaa tatccagcac cgagcgttca tcattaaatt cgacttcaaa
    21421 cagtgcgccg tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc ccagcagcag
    21481 ttctgcgcca ggttttggcg ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc
    21541 gagcatccgt tcaaccagca cttcaatctt gccgccgctg gctttacgcc caaacaggcg
    21601 cgccgggatc acgcgggtat tattaaaaac cagaagatcg ccggggttga gcttatcaag
    21661 taaatcggtg aaagtaccgt gcgtcagcgc gcccgtcggc ccgtccagcg acagtaaacg
    21721 acagctactg cgttcaggca tgggatagtg ggcaatcagg gattcgggca attcaaagga
    21781 gaaatcggta acgcgcatga cactgactca gactaaaata agaggcgggt agtctagtgc
    21841 cggggcgctc tccctgcaac ctttaccccc ttttggatag aatataatcg atgaattttt
    21901 tagctcacct gcatttagcc catctcgcgg aaagctcgct ttccggcaat ttactggctg
    21961 atttcgtacg cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct ggcattcata
    22021 tgcatcgacg tatcgacgta ttaaccgaca atctgccgga agtccgcgaa gcacgggaat
    22081 ggtttcgtag tgaaactcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    22141 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    22201 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    22261 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    22321 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    22381 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    22441 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    22501 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    22561 ttgcacggca ttgagcaacg gtgacgcggg tagcaccact tcacaagctt cgccacggtt
    22621 gattgccacc agtacacgct gctgattcag cacgcggaca aataccacca cgttatcttc
    22681 cgcatacagc acctgacagc cgccatgacg tagcgcctga cttttcttac gcagcgcaat
    22741 cattcgctgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    22801 tttacggcaa aacggatcgt ttttgccatc cagtcctact tcatcaccgt aataaatgca
    22861 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    22921 accgagcagc gttttaaatc gcgcagtatc gtggctgtcg agctgattaa acatacgtaa
    22981 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    23041 atcaatttgc tgcggatcgt aagagatatc ggtattggca agaaatcccc acaacgggaa
    23101 tgtgaagcca cgatagttca tggcggcatc ttccacatcg gcctgtaacc attgccgtgc
    23161 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    23221 gatcccagca acgtg
//
LOCUS       DAZFDP010000067        23158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFDP010000067 DAZFDP010000000
VERSION     DAZFDP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..127
                     /locus_tag="ACHJ1G_002677"
     CDS             8..127
                     /locus_tag="ACHJ1G_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4764511.1"
                     /translation="MVYGVFPNQDGTFTAMTYTKSKTFKTENGARRWLAKNTS"
     gene            548..769
                     /gene="kilR"
                     /locus_tag="ACHJ1G_002678"
     CDS             548..769
                     /gene="kilR"
                     /locus_tag="ACHJ1G_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO4764512.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            769..939
                     /locus_tag="ACHJ1G_002679"
     CDS             769..939
                     /locus_tag="ACHJ1G_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO4764513.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            1013..1288
                     /locus_tag="ACHJ1G_002680"
     CDS             1013..1288
                     /locus_tag="ACHJ1G_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764514.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            1387..4038
                     /gene="recE"
                     /locus_tag="ACHJ1G_002681"
     CDS             1387..4038
                     /gene="recE"
                     /locus_tag="ACHJ1G_002681"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO4764515.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            4031..4840
                     /gene="recT"
                     /locus_tag="ACHJ1G_002682"
     CDS             4031..4840
                     /gene="recT"
                     /locus_tag="ACHJ1G_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO4764516.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4897..5091
                     /gene="ralR"
                     /locus_tag="ACHJ1G_002683"
     CDS             4897..5091
                     /gene="ralR"
                     /locus_tag="ACHJ1G_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO4764517.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            5084..5272
                     /locus_tag="ACHJ1G_002684"
     CDS             5084..5272
                     /locus_tag="ACHJ1G_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4764518.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            5379..5660
                     /locus_tag="ACHJ1G_002685"
     CDS             5379..5660
                     /locus_tag="ACHJ1G_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4764519.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            5626..6702
                     /locus_tag="ACHJ1G_002686"
     CDS             5626..6702
                     /locus_tag="ACHJ1G_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764520.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(7095..7436)
                     /gene="yebY"
                     /locus_tag="ACHJ1G_002687"
     CDS             complement(7095..7436)
                     /gene="yebY"
                     /locus_tag="ACHJ1G_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4764521.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7449..8321)
                     /gene="copD"
                     /locus_tag="ACHJ1G_002688"
     CDS             complement(7449..8321)
                     /gene="copD"
                     /locus_tag="ACHJ1G_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4764522.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(8325..8699)
                     /gene="yobA"
                     /locus_tag="ACHJ1G_002689"
     CDS             complement(8325..8699)
                     /gene="yobA"
                     /locus_tag="ACHJ1G_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4764523.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8838..9068
                     /gene="holE"
                     /locus_tag="ACHJ1G_002690"
     CDS             8838..9068
                     /gene="holE"
                     /locus_tag="ACHJ1G_002690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4764524.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9170..9826
                     /gene="yobB"
                     /locus_tag="ACHJ1G_002691"
     CDS             9170..9826
                     /gene="yobB"
                     /locus_tag="ACHJ1G_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4764525.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9850..10512
                     /gene="exoX"
                     /locus_tag="ACHJ1G_002692"
     CDS             9850..10512
                     /gene="exoX"
                     /locus_tag="ACHJ1G_002692"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4764526.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10509..12569)
                     /gene="ptrB"
                     /locus_tag="ACHJ1G_002693"
     CDS             complement(10509..12569)
                     /gene="ptrB"
                     /locus_tag="ACHJ1G_002693"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4764527.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12778..13437)
                     /gene="yebE"
                     /locus_tag="ACHJ1G_002694"
     CDS             complement(12778..13437)
                     /gene="yebE"
                     /locus_tag="ACHJ1G_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4764528.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(13764..14120)
                     /gene="yebF"
                     /locus_tag="ACHJ1G_002695"
     CDS             complement(13764..14120)
                     /gene="yebF"
                     /locus_tag="ACHJ1G_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4764529.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14187..14477)
                     /gene="yebG"
                     /locus_tag="ACHJ1G_002696"
     CDS             complement(14187..14477)
                     /gene="yebG"
                     /locus_tag="ACHJ1G_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4764530.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14611..15789
                     /gene="purT"
                     /locus_tag="ACHJ1G_002697"
     CDS             14611..15789
                     /gene="purT"
                     /locus_tag="ACHJ1G_002697"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4764531.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(15845..16486)
                     /gene="kdgA"
                     /locus_tag="ACHJ1G_002698"
                     /gene_synonym="eda"
     CDS             complement(15845..16486)
                     /gene="kdgA"
                     /locus_tag="ACHJ1G_002698"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4764532.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(16523..18334)
                     /gene="edd"
                     /locus_tag="ACHJ1G_002699"
     CDS             complement(16523..18334)
                     /gene="edd"
                     /locus_tag="ACHJ1G_002699"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4764533.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(18569..20044)
                     /gene="zwf"
                     /locus_tag="ACHJ1G_002700"
     CDS             complement(18569..20044)
                     /gene="zwf"
                     /locus_tag="ACHJ1G_002700"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4764534.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            20382..21251
                     /gene="hexR"
                     /locus_tag="ACHJ1G_002701"
                     /gene_synonym="yebK"
     CDS             20382..21251
                     /gene="hexR"
                     /locus_tag="ACHJ1G_002701"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4764535.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21379..22821
                     /gene="pyk"
                     /locus_tag="ACHJ1G_002702"
     CDS             21379..22821
                     /gene="pyk"
                     /locus_tag="ACHJ1G_002702"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4764536.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22881..>23158)
                     /locus_tag="ACHJ1G_002703"
     CDS             complement(22881..>23158)
                     /locus_tag="ACHJ1G_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFO4764537.1"
                     /translation="RIVRTIGIGAGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSK
                     DSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         6183 a         5687 c         5637 g         5651 t
ORIGIN      
        1 cgcatctctg gtgtacggcg tgtttccaaa ccaggacggc acgttcaccg caatgacgta
       61 taccaaaagc aaaacgttta aaaccgaaaa tggtgcccgt cgctggcttg ccaaaaatac
      121 cagctaaacc atttattgga ttaattcaat attcttgctg taggggtata gccgaggcca
      181 ccaaagcccg gaggtggtga aataaaaccg ggcacaacac gaaggcgcat ttccggtatc
      241 cataaagagt cggtcttgtc tgttaaattt aaatggtggg agtgcgcctc cggttgtgaa
      301 taacaacact gctgtgtgta gtcttggcgg catcagtttt ttcttgaagt tcgactgatg
      361 tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc ggctaagcgc acgcggcaca
      421 gttaaaagtc atgttagtcc ttattggttt gggtgggaaa gccgactgta attgttaact
      481 ggttgcagtc acctggaggc accaggcacc gcatcaacaa agttcatttg taaaaatgga
      541 gataattatg attgcacatc acttcggaac tgatgaaata ccacgtcagt gtgtgactcc
      601 tggtgattat gttcttcatg aaggccggac atatatcgcc tcggcaaaca atattaaaaa
      661 gcgaaaactt tatattcgta acctgactac aaaaacatgc atttctgact gcatgattaa
      721 agtcttcctc ggtcgtgatg gtttacctgt aaaggcggag tcatggtgat gactaagaaa
      781 ataaaatgtg cttaccacct ttgcaataaa ggaattgaag aaagcaaaag cattaaaaga
      841 ccacttcatt tcatgcgtgg agttatccca acgacggaaa tgaaaaaata ttgtagtgaa
      901 aattgtgccg aaaaagacca gatggcacac gaactttaat taactgacta tgcgaaactg
      961 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aaatgattac
     1021 caattatgaa gtcactgttg taactaccga tgacattgtt cacgaggtta atctggaagg
     1081 aaagcgtatt ggctacgtga ttaaaacaga aaataaagaa accccattca ctgtggttga
     1141 tattgacggt ccatcaggca acgtaaaaac acttgatgaa ggtgtcacaa aaatgagtct
     1201 ggttcacatc ggaaagaatc tgcccgcaga aaaaaaagcc ggatttctgg caactctgat
     1261 tgcaatgaaa ttaaaaggtg aaatctgaaa aaagaaagcc tgcacaacgt gcaggcctga
     1321 gtgaagaacc tgggacattt attcatcact cgcagtaatt ttaatctgag ttgaggttaa
     1381 aaaacaatga gcacaaaacc actcttcctt ttacggaaag cgaaaaaatc atccggtgaa
     1441 cctgacgtcg tcctgtgggc aagcgacgat tttgaatcaa cctgtgccac tctggactac
     1501 ctgatcgtta agtcaggtaa aaaactgagc aactatttta aagctgttgc cacaaatttt
     1561 cctgtcgtta atgaccttcc ccctgaaggt gagatcgatt ttacctggag tgaacgctat
     1621 caactcagca aagactccat gacctgggaa ctaaaaccgg gagcagcgcc agacgacgtt
     1681 caccatcagg aggatgcacc ggaaaccgaa gaaccgacgg gaggccagga agaaaacgcg
     1741 caggcagacg cccacgagga ttgccaggat tgcgaagtct ctgtagccac tttgcggttc
     1801 acacagcgtc ttctgcacat ttttacgtat gcagccgggg atcgaaaata cctgcatcat
     1861 gccacccgtg aacaacgcaa acacattact gctcttgaga tggatcagga aaacagctat
     1921 gtccagaatc tgctgttggc catacgcagc atggcagaac cgacaactct ggataatgcc
     1981 gccctgctcc gcctgactga tgcaattaag gcagtgttct ctatcacgaa aaaacatcag
     2041 ccctatgaat ttaagaattt catttcagcc tggctggata ccgaacacat tgatcgcggt
     2101 cttctgacaa aagaatggcg aaaagggaat cgtgtttcac gcatcactcg cacggcttcc
     2161 ggtgctaatg ctggcggcgg gaacctcacc gatcgcggcg aaggtttcgt ccacgatctg
     2221 acatcactgg cgcgcgatgt agccactggc gtactggccc gttcaatgga cgtggacatc
     2281 tataaccttc atccggcaca cgctaaacgc attgaggaaa ttatcgctga aaataaacca
     2341 cccttttctg ttttccgcga caaattcatc accatgcctg gcgggctgga ttattcccgc
     2401 gccatcgtgg ttgcgtccgt gaaagaagca ccaattggga tcgaggtcat ccccgcacac
     2461 gtcactgaat atctgaacaa agtactgact gaaaccgatc atgccaaccc tgatccggaa
     2521 atcgtggata ttgcctgcgg tcgctcctcg gccccgatgc cgcaacgagt aacagaagaa
     2581 ggaaaacagg acgatgaaga aaaaccacaa ccatctggcg caatggcaga tgaacaggca
     2641 acggctgaaa cagtggaacc gaatgcaact gaacatcatc agaacacgca gccgctggat
     2701 gctcagtcac aggtaaattc tgttgatgcg aaatatcagg aactgcgggc agaactccat
     2761 gaagcccgga aaaacattcc atcaaaaaat cctgtcgatg ccgataaatt gcttgctgca
     2821 tcacgtggtg aatttgttga cggaattagc gacccgaacg atccgaaatg ggttaagggg
     2881 atccagactc gcgattctgt gtaccagaac cagccagaaa cggaaaaaat cagcccggat
     2941 gcgaaacaac ctgagccagt agtgcaacag gaaccggaaa tagtctgcaa tgcctgcggt
     3001 cagactggcg gggataactg ccctgactgt ggtgcggtga tgggcgacgc aacataccag
     3061 gaaacattcg gtgaagagaa tcaggttgaa gctaaggaaa aagatccgga ggaaatggaa
     3121 ggcgctgaac atccgcacaa tgagaatgct ggcagcgatc cgcatcgcga ttgcagtgat
     3181 gaaactggcg aagtcgcaga tcccgtaatc gtagaagaca tagagccagg tatttattac
     3241 ggaatttcga atgagaatta ccacgcgggt cccggcgtca gtaagtctca gctcgatgac
     3301 attgctgata ctccggcact gtatttgtgg cgtaaaaatg cccccgtgga cactacaaag
     3361 acaaaaacgc tcgatttagg aaccgctttc cactgccggg tacttgaacc ggaagaattc
     3421 agtaaccgct ttatcgtagc acctgaattt aaccgccgta caaacgccgg aaaagaagaa
     3481 gagaaagcgt ttctgatgga atgcgcaagc acaggaaaaa cggttatcac tgcggaagaa
     3541 ggccggaaaa ttgaactcat gtatcaacgc gttatggctt tgccgctggg gcaatggctt
     3601 gttgaaagcg ccggacacgc tgaatcatca atttactggg aagatccgga aacaggaatt
     3661 ttgtgtcggt gccgtccgga caaaattatt cctgaatttc actggatcat ggacgtgaaa
     3721 accacagcgg atattcaacg attcaaaacg gcttattacg actaccgcta tcacgttcag
     3781 gatgcattct acagtgacgg ttatgaagca cagtttggcg tgctgccaac tttcgttttt
     3841 ctggttgcca gcacaactgt tgaatgcgga cgttacccgg ttgagatttt catgatgggc
     3901 gaagaagcaa aactggcagg ccagcaggaa tatcaccgca atctgaggac cctggctgac
     3961 tgcctgaata ccgatgaatg gccagctatt aaaacgttat cactgccccg ctgggctaag
     4021 gagtatgcaa atgactaagc aaccacctat cgcaaaagcc gatctgcaaa aaacccaggg
     4081 aaaccgtgca ccagcagcag taaaaaataa cgacgtgatc agctttatta atcagccatc
     4141 aatgaaagag caactggcag cagctctccc acgccatatg acggctgaac gaatgatacg
     4201 tatcgccacc acagaaattc gtaaggttcc ggcgctagga aactgtgaca ccatgagttt
     4261 tgtcagtgcg atcgttcagt gttcacagct cggccttgag ccaggtagcg ccctcggcca
     4321 cgcatattta ctgccttttg gtaataaaaa cgaaaagagc ggtaaaaaga acgttcagct
     4381 aatcattggt tatcgcggca tgattgatct ggctcgccgt tctggtcaaa tcgccagcct
     4441 gtcagcccgt gttgtccgtg aaggtgacga gtttagtttt gaatttggcc ttgatgaaaa
     4501 gttaatacac cgcccgggag aaaacgaaga tgcaccagtg acccacgtct atgctgtcgc
     4561 aagactgaaa gacggaggga ctcagtttga agttatgacg cgcaaacaga ttgaactggt
     4621 gcgcagccag agtaaggctg gtaataacgg gccatgggta actcactggg aagaaatggc
     4681 aaaaaaaacg gctattcgtc gcctgtttaa atacctgcct gtctcaattg aaatccagcg
     4741 tgcagtatca atggatgaaa aggaaccact gacaatcgat ccggcagact cctctgtatt
     4801 aaccggggaa tacagtgtaa tcgataattc agaagaataa ttcagcctgg cggtgtaatg
     4861 caccgccaac gtgagacagt ttttatgaca aaaattatga gatatgacga tgttaaacca
     4921 tgtccgtttt gtggttgtcc atctgttaca gtgaaagcaa tttcaggata ttaccgggca
     4981 aaatgcaacg gatgcgaatc ccgaactggc tatggtggaa gtgaaaaaga agcactcgaa
     5041 agatggaata aacgaactac tggaaataat aatggaggtg ttcatgtata aaataactgc
     5101 cactattgaa aaagaaggtg gcactcctac taactggaca agatattcaa aatctaaatt
     5161 aacgaaatca gaatgcgaaa aaatgctctc agggaaaaaa gaagcaggcg tgtccagaga
     5221 gcagaaagta aagctgataa attttaattg cgagaaactt ctgtcctcgt gagttgcatt
     5281 atatacaaat tagaacttca tagctgatta ttaaaaatca accacacccg ccagtattct
     5341 gtatatttac tggcggtcat atcgtaagag gtatggcaat gaatcttgtg acactcaaaa
     5401 cgtggggaaa actcagatat ccggataacc caccatcaat atcaacgctg agacgatggg
     5461 caaggaatgg aaacatttat cctgcacctg aactacacgg gaggagttac agggtggttc
     5521 cggaagcttt ctatatcaac ccaaataagg ttgataccga tataacacac catcagccta
     5581 atgggcgaca agggagagac agtccgttac tggagaagtt aaaacatgca gcggaaaaaa
     5641 tacgatccca atttacctaa aaacttaaca tatcgaagga gggacaaagc atattactgg
     5701 cgcaaccctc tgacgaaaga agaatttaca ctaggtaaaa tttcaagaag agatgcagta
     5761 gcgcaggcaa ttgaagcaaa tcattatata tacaaaaact actctcctgc tgccttaatt
     5821 gaaaaactaa aagggttcga ctcatttact atggcagact ggattgaacg ttacaaaacg
     5881 attcttataa ggagaaaagt gtccagaaat acttataaaa ttcgggtaaa tcaactggag
     5941 acaataaaag aaaaattagg aggggtttta ctgacagaaa taaccactcg ccatattgcc
     6001 gagtttcttg atttgtggat tgaaggaggg aaaaacacga tggcaggatc aatgcgttct
     6061 gtgttatctg atatgttccg cgaggccatt gttgaaggac gtatatctca aaatccagta
     6121 acgccaacaa gagcaccgaa aatagtagtt acaagagaac ggctgaaact aaagacatac
     6181 aactgcatca gggaggcagc agatcaactt ccgacatggt tcccattagc tatggactta
     6241 gcccttgtaa caggacaacg tcgcgaagac ataacaaata tgcggttcag tgagatttat
     6301 gatgatcgtc tccacatcag gcaaattaag acaggaatga tgattgctat ccccctgtca
     6361 ctcagccttc ctgtcgctgg tttacgactt ggtacagtag ttgaacagtg ccgcatggta
     6421 agtagggggg attatctaat cagtgccggg attagaaaaa acagccctga cggcagcatt
     6481 cacccggacg gcctgacaaa gaaatttgtc gcagccagaa aattaacagg tatccagttc
     6541 agtgaaaacc caccaacttt tcacgagatc agaagcctgg ctggacgatt gtacaaagaa
     6601 acatgtgggg aagaatttgc tcaacgtcta cttggccaca catcggagaa gacaacaaaa
     6661 atgtatcttg atgagagaga aaaaacgtac ttactgctct gattttaacg taaatggatt
     6721 gttaaatgtg ttttggttgt gatataacca aaaaagaccg gaatacagaa attcgagtaa
     6781 atttcgagta atttcgggga gacgtttgca actaattgat tttaaataca attaaaaaaa
     6841 gaccgaatac gattcctgta ttcggtccag ggaaatggct cttgggagag agccgtgcgc
     6901 taaaagttgg cattaatgca ggcttagttg ccttgccctt taagaataga tgacgacgcc
     6961 aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg tggtggtcat cagctgaaat
     7021 gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt tttttattgg aaatcaaaag
     7081 gctattttag gtaattaaca gagtttttca gctcgttcta taaacggtgc cagactcatt
     7141 ttttcgccgg gattgttagg atcatcaatc tgaatcaccg aaatgggttg ggctttagtc
     7201 ttcccactgg caacttcctt ttgtgcgata tcgtttaaag gatactgcac gagggtactt
     7261 gggttaatga catacaaagc attacccggt ctgcaagtca gcatcacctc ttcgcgatta
     7321 aacgcccatt tgtctttacc cacttcaaaa cgactgacgg taatcacctg cggtgcagcc
     7381 agcgccgctg cagaactggt gagtaacaga aacgtcagaa tacttttttt catcattttc
     7441 gtaatccatc aaaaaggttc gagagtcgca agcaggctga tgatcaccag cactaccgca
     7501 cctatcgccc attcgagttt agtcatccac tcaaaccagg gtgccgcacg atcttcatct
     7561 tgcctcatgc gcggtacaag aacataacga ttagccagcg caatgaccac cataatcatc
     7621 accaggatgg ctttgagcaa taagagttgg ccccagtaag tcgtaagcgt ggggggaaat
     7681 ccggtaatca acaatgcatt aagcacgccg cttgccagta cgccgatcac cgcaaaatgc
     7741 ccgcaccagg aaaaacgcat cagcgcctga atggcctggt gtcgccaacg gcctttgatg
     7801 agttgcatac accagagcac cggcagtaat cccccaaacc acgccgccgc acaaatcagg
     7861 tgaatcgcgt gattagtctg atggattttc gccgttaccc cttcattcag tgtcgcatgc
     7921 ccgattcccg ccagcagaat aaattgcgcg gtagtgagca taaacagcaa acgtggcata
     7981 ttccgcggtt gcataagggc gacgatcaac gttacgaggg cgagaacaat ttgccataac
     8041 cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa tgtttggcga aaatacatct
     8101 gcccatcccg ttcccatcaa cccgccctga acggcgagca ttgcagtggc actgatcaaa
     8161 ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct tcgccagcaa gcgacgaatc
     8221 gttaaggggg ccagccaggc accgtacatt gcgaagccaa agaccagcat cagcgaggtg
     8281 aaatggataa atcgcagcgc gatccaggta aacgccagca tgatttattt cacgctaaag
     8341 gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa catgccagtc aacgatgtag
     8401 gttccgggtt tcagcgaatc ggccagtgga acaatcagtt gcttttggtc ctgctcattt
     8461 cgcttcgccg gtaatgtttt aatattttcg ttttttggcc ccgtgatttt tgcaccactg
     8521 aatcctgttt caacaccttc cgagaagttt aaagtgattg cctgcggtgc agctgtcact
     8581 tgcgcgtttg ccgcaggata ctgatgcgtt aaatgcgcat gtgcccagac ggaaggtgtc
     8641 accaacgaag tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt tgaagtcata
     8701 ttatctattc ctttttgtaa taactttttt acagagcata accttgtcta atgtctgagt
     8761 cgaggatcat caattccggc ttgccatcct ggctcactct tagtaacttt tgcccgcgaa
     8821 tgatgaggag attaagaatg ctgaagaatc tggctaaact ggatcaaaca gaaatggata
     8881 aagtgaatgt cgatttggcg gcggccgggg tggcatttaa agaacgctac aatatgccgg
     8941 tgatcgctga agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg tttcgcgagc
     9001 ggcttattgc ccaccgtttg gcttcggtca atctgtcacg tttaccttac gagcccaaac
     9061 ttaaataaaa cttatacaga gttacacttt cttacataac gcctgctaaa ttatgagtat
     9121 tttctaaacc gcacccataa tttgcagtca ttttgaaaag gaagtcatta tgtcgttctg
     9181 gaaagttgca gcagcgcaat atgagcctcg taaaacctca ctcaccgaac aggtagctca
     9241 tcatctggag tttgtgaggg ccgccgccag gcaacaatgt cagctgttag tttttccatc
     9301 gctttcttta ctggggtgtg attattcgcg acgtgccctt cctgccccac ccgatctctc
     9361 actgttagac ccgctttgtt atgccgcaac gacctggcga atgaccatca ttgccggcct
     9421 tcccgttgaa tataacgatc gctttattcg tggaattgcg gtgttcgcac cgtggcgaaa
     9481 aacgcctggg atctaccatc agagtcatgg tgcatgtctg ggtcgtcgtt ccagaaccat
     9541 cacggtggtc gatgaacaac cgcaaggtat ggatatggac ccgacctgtt cactgtttac
     9601 tacagggcaa tgtctgggag aacccgacct gctggcgtcg gcccgtcgct tacaattttt
     9661 ttcacatcag tactcgattg ccgtgctgat ggcaaatgcc cgtggtaaca gtgcactatg
     9721 ggatgaatat ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt tagtcggtca
     9781 gcgttcatca cagggttggc aaggcgatat cattccatta cgctaggctt tttcggcctg
     9841 gagcatgcca tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg agggatcgtt
     9901 gagattgcct ctgttgatgt cattgacgga aaaatcgtca accccatgag ccacctggtg
     9961 cgccccgatc gtcctattag tccacaagcg atggcgattc atcgcatcac cgaagccatg
    10021 gtcgccgata aaccgtggat tgaagatgtg atcccacact attacggtag tgaatggtat
    10081 gtcgcgcata acgccagctt tgaccgccgc gtactgcctg agatgcccgg tgagtggatt
    10141 tgcactatga aactggcccg tcgtttgtgg cctgggatca agtacagcaa tatggcgtta
    10201 tataaaacac gcaagctcaa tgtacagacg ccgccgggcc tgcatcatca ccgcgcgttg
    10261 tatgactgtt atatcaccgc cgcgttgctt atcgatatta tgaacacctc cggctggacg
    10321 gcagaacaga tggccgatat caccggacgt ccgtcgttga tgacgacctt cacctttggc
    10381 aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta tctgcgctgg
    10441 ttatttaata acctggacag catgagcccg gagctgcgtt taacactgaa acattatctg
    10501 gaaaatactt agtccgcagg cgtagcgggt aatgttccct gcgccagcgc gaccagaaaa
    10561 gcatattcca tcgctacgcc ttcgtacgat ttaaagcgac cagatttacc gccatggcct
    10621 gagtccatgt cggtacagag caataaaaga tggtcatcgg ttttcagctc gcgcaattta
    10681 gcgacccatt ttgccggttc ccaatattgc acctgagaat cgtgcaaacc ggtcgttacc
    10741 agtaaatgcg gataagcctg tgcggtgacg ttgtcatacg ggctgtagct tttcatgtac
    10801 tcgtaatatt gcggatcctg cggattaccc cactcttcaa actcaccagt ggtaagagga
    10861 attgattcat caagcatcgt tgttacaaca tcaacaaacg gtacctgggc gataacgccg
    10921 tggaataatt ccgggcgttg attaattgca acgcccatca acatgccccc cgcactcccg
    10981 cccatcgcat aacaaagcga aggagagcca tagcccagtt ttaacaatgc atcgcaggca
    11041 tcaagataat cattaaacgt atttttcttc ttcagaaatt ttccgtcttc gtaccattgt
    11101 tgccccagct caccaccgcc gcgaacatgg acaatggcgt agacaaagcc acgatctaac
    11161 aaactcaagc ggctaaaact gaaatcggca tcaatacttg cgccgtaaga accatagcca
    11221 tacaccagca acgggttgtg tcctttgcga aaatgtttgc gatggtagac caacgaaacc
    11281 ggaacttcga cgccatcacg ggcgactatc cacaggtgtt cactgcggta attcgccgca
    11341 tcaaaaccgg gcacttctgt ttgttttaat acacgacgct caccggtatc catatccagt
    11401 tcaaacaaag tgtctggtgt ggtcatggaa gaataaccat aacgcaatcg cgcggtttca
    11461 ggttctggat tgtaggcaat ccaggtcaca taggctggat catcaaaggc aataccaatg
    11521 acttcccggg tcttgcggtt aatttggcgc aaactggtta acccgcgctg acgctcttca
    11581 accaccagcc agtcggtaaa cagcgtaaac ccttccagca tgatgttttc gcgtggcgga
    11641 attaactctt cccactgttg ctcatcacgc atacgggtac ggtataagcc aaagtttttg
    11701 ccgtggcggt tggaacgtag ataaaaacga tgctggtagt gatcaaggct gtattcgtga
    11761 tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg ccatttccgc gtccagcagg
    11821 cgaacttcac tggtggtggc gctggccaaa tgaatgacta catagtgctt cgaggtcgtt
    11881 ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt agatcagttt atcttgcgat
    11941 gctggcgtac cgatggcgtg acgccagacc tgataaggca gcagcgtcac cggatgcttg
    12001 cgaacatagt agaaagtcca ggagtcattt gcccagacaa agctgggttc aacgttatcc
    12061 agcagttccg ggtaccagtt accagtttcc agattacgaa aacgaatgcc gtactggcgt
    12121 cgggaaagaa aatcttctgc cagcgccata atggtgttat cgggcgtaat cgccattccg
    12181 cccatcgaat aaaactcact atgagctgcg cgcttattgg catcgagcaa tgtttcccac
    12241 tcatcccact cttcactgaa tgccgattga cgctggtaga tagcatattc acagcctggt
    12301 tcataaatat gccgatagcg gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc
    12361 ggaatgcggt cgatgatttc ctttaagatg cgatcctgca aggcttgttg tgaggccatc
    12421 acccgatgac cgtaactatt ttcttgttgc aggtagtcca ggacttctgg ctgagaacgc
    12481 gtatcgtccc gcagccagta gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg
    12541 tggggaatgc gggcggcttt tggtagcatg ttattgttct ttctggttga aacatcttat
    12601 aagggtggca aaactcaccg gggatgcaag cgaaacaggg gagtcattgc ttagatgatg
    12661 acaggtaatg gcgcggatat cgaatgttat gcagacagag aaaatcagcc tgttcaaatg
    12721 gctgtgcgat tctggatagc ccgaaatagt caacttcagg ctatccagag agcggaatta
    12781 ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca atgccgtcac gaacatcctg
    12841 ggggattttc agcgcgtcac ccagtgcatt caggtaactg cgttccataa aatggtcaat
    12901 atcaatagcc gcgcaactca ggaaatagat ttccagcgcc tcttcttcat tgcggacccc
    12961 ggtagccagg cgttgtggat ccagcggttg ttcgattgcc tgctcaatga gtacacgccc
    13021 ctgctcttcc acgccggctt cacgcaattg ctggtcgatt gccgcacgtt ctttggcatc
    13081 aatatgacca tcacttttag cggcaaagac cagcgcaagg atcaaacgtt ctgtacgctc
    13141 atccagcggc gtactttgcg cgccaaactg cggttcgtcc tgatgcgccg cgcgaatttt
    13201 atctttgtat ttattccaca gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc
    13261 gttggtgcca tatttcgtaa gaagtttacg tgctgattta tttgcgacca gcagcccagc
    13321 cagtccgcct aatgcgcctg gcactaacag tttgcccaat ccctgatccg cagacgagga
    13381 ggtagaagaa ctgctttgcc caagcaggga ttgcagttga tttaaccagt tagccatatt
    13441 tgctcctcaa taaccatttt tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt
    13501 atatcgagac aaaagatgcg caaataagcg gggcggtagg ccgttattca aagaaattcg
    13561 cgccaggtaa ggtatttact gtatatcagg tgataagggt attttaggtg aaagtagttt
    13621 gtcgttttga gtggcattgt ttgatgctga ttgcccgatg cgacgctgtt gcgtcttctc
    13681 aggcctacga ttgtggtgcg ttctggggcg gacaaggcac tcgcgccgca tccgccaacg
    13741 gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt ccgccattcc cgctttgcag
    13801 tccacgctga cctggtaatg aatatcggca cttttaccac gcacggttag cggtactgac
    13861 catttatcat ctttaccctg aatgtcctgc aaactgaccc aagccacggg atcggcctga
    13921 ccgacaattt tttgatcatc tgcccaacgc gccacgcgat tttgttgata atcacgtttt
    13981 acgctcgcgg caattccggc agcatccaga ccttcacact ttgggaaagt gaccgacttg
    14041 ctggtttcat tattggcagc gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa
    14101 aacgcccctc tttttttcat gtttttctcc atagcacaat gattcaggag aaagcatggt
    14161 acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat
    14221 tcagcaccga cgacctgtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca
    14281 tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact
    14341 ggagaatttg tcagccaggt gtcaagcaga tccgccgtat caagcatttt atcataagca
    14401 tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat
    14461 ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac agtatatttc
    14521 ttttcggttg agaaatcaac atcagcaata aagacacacg caaacgtttt cgtttatact
    14581 gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg
    14641 gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag
    14701 tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg
    14761 catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg
    14821 gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg
    14881 ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta
    14941 acgatgaatc gcgagggtat ccgtcgcctg gcggcagaac agctgcagct gcccacttcc
    15001 acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgccgc cattggctat
    15061 ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt tattcgctct
    15121 gcagagcaac ttgctcaggc atgggaatac gctcagcaag gcggtcgcgc cggagcgggc
    15181 cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc
    15241 gcggtggatg gcgtccattt ctgtgcacca gtaggtcatc gccaggaaga tggcgactac
    15301 cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt
    15361 gcccgtaaag tggtgctggc actgggcggt tatggtctgt ttggtgtcga gctgtttgtc
    15421 tgtggtgatg aggtgatttt cagtgaggtc tcccctcgtc cacatgatac cgggatggtg
    15481 acgttaattt ctcaagatct ctcagagttt gccctgcatg tacgtgcctt cctcggactt
    15541 ccggttggcg ggatccgcca gtatggtcct gcagcttctg ccgttatcct gccacaactg
    15601 accagtcaga atgtcacgtt tgataatgtg cagaatgccg taggcgcagg tttgcagatt
    15661 cgtttatttg gtaagccggc aattaatggc agccgtcgtc tgggggtggc actggctact
    15721 gcagagagtg ttgttgacgc cattaaacgc gcgaagcacg ccgccggaca ggtaaaagta
    15781 cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atccgcgatc gggcattttg
    15841 acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaata cggtcgtaat
    15901 cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt
    15961 tcagcgccag gtagtcacgg tagttagccg gagaaatacc acctgtcggg cagaaacgga
    16021 cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta gcttcagccg
    16081 ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc
    16141 tgatccccgg aatcagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca
    16201 gacccgggct aattgcgaac tgtgcacccg cttcggtgac ttctgccagc tgctgtggat
    16261 tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag
    16321 cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca
    16381 acgcttttgc catcggcact gcgtgttcca gtttttttac cacgataacc ggtacaaccg
    16441 ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc
    16501 gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg
    16561 acagtttttc acgcaaggcg ctgaataatt cacgtcctgt ccccacgcgt gacgcgctca
    16621 ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt
    16681 cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc
    16741 catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga
    16801 gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg
    16861 gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa
    16921 cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt
    16981 caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca gaggttttca
    17041 taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt
    17101 gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca
    17161 gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg
    17221 tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc
    17281 cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca
    17341 gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata ccggccgcgc
    17401 gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa
    17461 cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac
    17521 cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg
    17581 gagaatccgg atgaacaaaa gaagagcctg gcaactgcat ccccataaat tccaccacca
    17641 tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg
    17701 cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac
    17761 gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag
    17821 gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac
    17881 cgaggaacag agcaccatca aacatgttat gggacagccc gaccgccgca gacatcgcta
    17941 tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg acaccatcac
    18001 acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc agggctttac
    18061 gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg
    18121 tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc
    18181 aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgaag
    18241 ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga
    18301 ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag
    18361 agcaggatga ttcacaacgc gtttcattca gaggatttat gactgaaacg cctgtaaccg
    18421 gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc
    18481 gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta
    18541 aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa cgaccatcac
    18601 gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg
    18661 catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca
    18721 cttcgtcgcg acgaacaaac agtgcctgaa taccacgcat ggtttccagc agcaaacgtt
    18781 cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga tccagcttgg
    18841 tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc tggatatcca
    18901 cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgatt
    18961 ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg
    19021 gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt
    19081 cgacgcggat cgctacgaag gtttctgtat tgctgctctt gttcgcgccc tcttcttcca
    19141 gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgcccg cgtacggttt
    19201 tttcgcgtac gttggagcgg tcgatgcggc gcagagactt cagtactttc actttttcat
    19261 cgcggatgct gtctgcgctc aggtcagacg gcggagacat cgcaatcatg cagagaattt
    19321 gcagcaggtg gttttggatc atatcgcgca tctgaccggc tttatcaaaa tagccccagc
    19381 gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt
    19441 cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt
    19501 ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct
    19561 gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc
    19621 gtgccggttt agcattcagt ttagcctcgc caagcccttt gcaaattgcg ccaaaagtgc
    19681 tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga
    19741 gacggctgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca
    19801 gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga
    19861 caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt
    19921 ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg
    19981 caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg
    20041 ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata cttgtaattt
    20101 tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc
    20161 gtgcgattac ggtaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc
    20221 cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa aataacaact tttttatctg
    20281 cttttgtcat taacacagca cacaggcgta atatctgaca aaactacatt tcgatttctt
    20341 tcagtgcgga aatcgtcatt acccgtgagt ttctttacat catgaatatg ctggaaaaaa
    20401 tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag gtcattctgg
    20461 cttcgcccga taacgcgatc cattcgagta ttgctgctct ggcgctggaa gccaatgtta
    20521 gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt cctgatttta
    20581 aacttcatct ggcacaaagt ctggcgaatg gcactcccta tgttaatcgc aatgtcaatg
    20641 aagatgacag cgttgaatca tacacaggga aaatatttga gtccgcaatg gcaacgcttg
    20701 atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac ttgctcactc
    20761 aggcaaaaaa aatcgccttt ttcggattag gctcttcagc cgccgttgcc cacgatgcga
    20821 tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc gtgctgcaac
    20881 gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct cacactggaa
    20941 gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga aaacgacgcc atggtgattg
    21001 ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt accctcgacg
    21061 taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag ctgaccgtga
    21121 tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga gataacttga
    21181 agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa gcagttactt aatttaagtg
    21241 acgatcgcta aaaacgactg tcactgtcct aatcttatac gacatccgaa tgagattaat
    21301 ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac ctggttgttt
    21361 cagtcaacgg agtattacat gtccagaagg cttcgcagaa caaaaatcgt taccacgtta
    21421 ggcccagcaa cagatcgcga taataatctt gaaaaagtta tcgcggcggg tgccaacgtt
    21481 gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcgcgc ggataaagtt
    21541 cgtgagattg ccgcaaaact ggggcgtcat gtggctattc tgggtgacct ccaggggccc
    21601 aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg ggataaattc
    21661 ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac
    21721 aaaggcctgc ctgctgacgt tgtgcctggc gacatcctgc tgctggacga tggtcgcgtt
    21781 cagttaaaag tactggaagt tcagggcatg aaagtgttca ccgaagtgac cgtcggtggt
    21841 cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc tgaagcgctg
    21901 accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga ttacctggct
    21961 gtctccttcc cacgctgcgg cgaagatctg aactatgccc gtcgcctggc acgcgatgca
    22021 ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg cagccaggat
    22081 gcaatggatg acatcatcct cgcctctgac gtggtaatgg ttgcacgcgg cgacctcggt
    22141 gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg tcgtgcgcgt
    22201 cagcttaacc gcgcggtaat cacggcgacc cagatgatgg agtcaatgat tactaacccg
    22261 atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg tactgacgct
    22321 gtgatgctgt ctgcagaaac tgccgctggg cagtatccgt cagaaactgt tgcagccatg
    22381 gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg
    22441 gacgttcagt tcgacaatgt ggaagaagct attgccatgt cagcaatgta cgcagctaac
    22501 cacctgaaag gcgttacggc gattattacc atgaccgaat cgggtcgtac cgcgctgatg
    22561 acctcccgta tcagctctgg tctaccaatt ttcgccatgt cgcgccatga acgtacgctg
    22621 aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc taatgacggc
    22681 gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt gatgtctggt
    22741 gacctggtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac taataccacg
    22801 cgtattttaa cggtagagta atctcacctg ccccggccgc tgatgaccgg ggtatttagc
    22861 aacacataaa ttaggactac tcattcaatc caatcataat cacagccccc tgtaaaatga
    22921 atacagggat agcctgaatg gagatctaaa tagcttgcaa gacgtcgcat acccgcttcc
    22981 tctgcaacac tatatccaat aactgccatc ggtaagccac tatctttgga gtattgtgtc
    23041 tgcacccatg ttttaattga atcgttaatc gtaatataat aatcagcctg atattccata
    23101 tattgtattg gatcacaaaa acagcctgcc ccaatcccaa tagttctaac tattctgg
//
LOCUS       DAZFDP010000068        22538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFDP010000068 DAZFDP010000000
VERSION     DAZFDP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            316..1215
                     /gene="yegS"
                     /locus_tag="ACHJ1G_002704"
     CDS             316..1215
                     /gene="yegS"
                     /locus_tag="ACHJ1G_002704"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4764538.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1297..2070)
                     /locus_tag="ACHJ1G_002705"
     CDS             complement(1297..2070)
                     /locus_tag="ACHJ1G_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4764539.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2176..3216)
                     /gene="gatD"
                     /locus_tag="ACHJ1G_002706"
                     /pseudo
     CDS             complement(2176..3216)
                     /gene="gatD"
                     /locus_tag="ACHJ1G_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3264..4619)
                     /locus_tag="ACHJ1G_002707"
     CDS             complement(3264..4619)
                     /locus_tag="ACHJ1G_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4764540.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4623..4907)
                     /gene="gatB"
                     /locus_tag="ACHJ1G_002708"
     CDS             complement(4623..4907)
                     /gene="gatB"
                     /locus_tag="ACHJ1G_002708"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4764541.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4938..5390)
                     /gene="gatA"
                     /locus_tag="ACHJ1G_002709"
     CDS             complement(4938..5390)
                     /gene="gatA"
                     /locus_tag="ACHJ1G_002709"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4764542.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5400..6662)
                     /gene="gatZ"
                     /locus_tag="ACHJ1G_002710"
     CDS             complement(5400..6662)
                     /gene="gatZ"
                     /locus_tag="ACHJ1G_002710"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO4764543.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6691..7545)
                     /gene="gatY"
                     /locus_tag="ACHJ1G_002711"
     CDS             complement(6691..7545)
                     /gene="gatY"
                     /locus_tag="ACHJ1G_002711"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4764544.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7855..8907)
                     /gene="fbaB"
                     /locus_tag="ACHJ1G_002712"
     CDS             complement(7855..8907)
                     /gene="fbaB"
                     /locus_tag="ACHJ1G_002712"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4764545.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9164..10441
                     /gene="yegT"
                     /locus_tag="ACHJ1G_002713"
     CDS             9164..10441
                     /gene="yegT"
                     /locus_tag="ACHJ1G_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4764546.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10438..11442
                     /gene="yegU"
                     /locus_tag="ACHJ1G_002714"
     CDS             10438..11442
                     /gene="yegU"
                     /locus_tag="ACHJ1G_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4764547.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11439..12404
                     /gene="yegV"
                     /locus_tag="ACHJ1G_002715"
     CDS             11439..12404
                     /gene="yegV"
                     /locus_tag="ACHJ1G_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4764548.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12378..13124)
                     /gene="yegW"
                     /locus_tag="ACHJ1G_002716"
     CDS             complement(12378..13124)
                     /gene="yegW"
                     /locus_tag="ACHJ1G_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4764549.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13176..13994)
                     /gene="yegX"
                     /locus_tag="ACHJ1G_002717"
     CDS             complement(13176..13994)
                     /gene="yegX"
                     /locus_tag="ACHJ1G_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4764550.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(14059..14859)
                     /gene="thiD"
                     /locus_tag="ACHJ1G_002718"
     CDS             complement(14059..14859)
                     /gene="thiD"
                     /locus_tag="ACHJ1G_002718"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4764551.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14856..15644)
                     /gene="thiM"
                     /locus_tag="ACHJ1G_002719"
     CDS             complement(14856..15644)
                     /gene="thiM"
                     /locus_tag="ACHJ1G_002719"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4764552.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15679..15775)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15867..16139)
                     /gene="rcnR"
                     /locus_tag="ACHJ1G_002720"
     CDS             complement(15867..16139)
                     /gene="rcnR"
                     /locus_tag="ACHJ1G_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4764553.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16260..17084
                     /gene="rcnA"
                     /locus_tag="ACHJ1G_002721"
     CDS             16260..17084
                     /gene="rcnA"
                     /locus_tag="ACHJ1G_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4764554.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17303..17641
                     /gene="rcnB"
                     /locus_tag="ACHJ1G_002722"
     CDS             17303..17641
                     /gene="rcnB"
                     /locus_tag="ACHJ1G_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4764555.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17723..18757)
                     /gene="yehA"
                     /locus_tag="ACHJ1G_002723"
     CDS             complement(17723..18757)
                     /gene="yehA"
                     /locus_tag="ACHJ1G_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO4764556.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18771..21251)
                     /gene="yehB"
                     /locus_tag="ACHJ1G_002724"
     CDS             complement(18771..21251)
                     /gene="yehB"
                     /locus_tag="ACHJ1G_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4764557.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21267..21941)
                     /gene="yehC"
                     /locus_tag="ACHJ1G_002725"
     CDS             complement(21267..21941)
                     /gene="yehC"
                     /locus_tag="ACHJ1G_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4764558.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(22022..>22538)
                     /gene="yehD"
                     /locus_tag="ACHJ1G_002726"
     CDS             complement(22022..>22538)
                     /gene="yehD"
                     /locus_tag="ACHJ1G_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4764559.1"
                     /translation="VFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSIN
                     MPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKEIA
                     SSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDV
                     LSSLVMRVAQD"
BASE COUNT         5691 a         5721 c         5205 g         5921 t
ORIGIN      
        1 acaacatttc ctacataagt aggaattacg gacattgagg cccttcaggg taactccatg
       61 ggggctttaa tatatttgga atagtatatt taagatgcca ctgtttggtt gaatattagg
      121 catatgctct tttttgaaat ttatcggtgg cagccgttag tattcgctgt ccccattgca
      181 agctcctggt ggtaaccact gaatcctcca tacttgaact gactttttat cctccgactt
      241 tcatcctgtt ctgactccac cttttgtttt ctgctctaca ctatctacag accaatcata
      301 aaggcacata cgatcatggc agaatttccc gccagcttac tgattcttaa tggcaaaagt
      361 actgacaatc tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc
      421 catgtgcggg tcacctggga gaaaggcgat gccgcacgat atgtagagga ggcccggaag
      481 ttgggcgtcg caacggtgat tgccggtggt ggcgatggca ccattaatga agtttctacg
      541 gcgttgattc agtgtgaggg ggatgacata cccgcgctgg gaattttgcc attaggaacc
      601 gccaatgatt ttgccaccag tgtagggatt cctgaggcac tggataaggc gctgaaactg
      661 gcaattgccg gtaacgccat tgcgatagat atggcgcagg tcaacaaaca aacctgtttt
      721 attaatatgg cgacaggcgg atttgggacg cgtattacca cagaaacgcc ggaaaaatta
      781 aaagccgcgc tgggtggcgt ctcttacatc attcatggct taatgcgcat ggatactctg
      841 caaccggacc gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc
      901 attggtattg gtaacgggcg tcaggccggt ggcggtcagc aattgtgtcc gaacgcgtta
      961 attaacgatg gcttgctgca actgcgcatt tttaccggcg atgaaattct tccggctctc
     1021 gtatcaacct taaaatctga cgaagataac ccgaatatta tcgaaggcgc ttcgtcgtgg
     1081 tttgatatac aagccccaca cgaaatcact tttaatcttg atggcgaacc gttgagcgga
     1141 caaaactttc atattgaaat acttccggcg gcgttgcgtt gtcgattacc accagattgt
     1201 ccattattgc gttaatcaaa ctattctctc ttgaaccagg gagaatccga aatgccggat
     1261 gcggtgtaaa taccttatcc ggcatacaga acaatactac accgtaatta attttacccg
     1321 tgtcttctgc aatgccagtt tatccacctc tgatacagca tcatcggtaa taataatgtc
     1381 aattttttcg attggcagta cctgattaaa accgcgacgg ttaaatttcg atgaatcgag
     1441 aacggcaatc actttattag cggcggtgac catcgcaccg ctaatcgaat aaccttcatt
     1501 aaaggtcgta ataccattaa ccgcatcaat accatcagca ccgacaaaca ttaaatcggc
     1561 attaatatcc tgcagggaac gttcagcaat agatccatgc atagatcgcg ttttatggcg
     1621 gaccgtgcca ccgcagacga ccagagtaat gtctttattt tcggaaaggg caaacgcagc
     1681 cgggagactg ttggtaatca ccgtgatatt tttggcggtc attaatcctt cggcgataag
     1741 cattgtggta cttccgctgt cgagaataac ggtcatccct tcatggatca ttttgactgc
     1801 cgcctgcgct attctgtttt tcggcgcgct ggcgagctga aatcgctctt taaacccgac
     1861 ttcctgggtc tcggtttcac tattaccgga cattattttc gccgcaccgc catgaaagcg
     1921 cgtaaccacg cctttttgtt cgagaaagcg caaatcggca cggattgtcg cttccgaggc
     1981 agcaaatact cccgccagat cctgaacaag cacggttccc tgttcattca ctaattggat
     2041 gatcttattc cttcgctcga atgaattcat cgtcatttac ccatattcaa ttgtggctag
     2101 tgtaaacgaa gggggaccat tggtgaatga tctgttttgc acaataccgg gccagcatca
     2161 cgctggcccg cggtttcagg gaatgagcaa cactttgccc ggcatagcat tacgagcgat
     2221 gtcacgcacc gcctgggcga agctttcaaa gcttccacgg tgagcgatta atggctccag
     2281 gcttaactta cgttctgtca gcaaccggct cgccgtttcc cactcctgcc ccggccaagg
     2341 gctggagtag ttcatccaac tgccgataac cgtcagctct ttacgcaata ttttgccaaa
     2401 cgttgccgat gttaaatgca gatcctgatg caacgtgccc accagcgcca gttgggcatg
     2461 aggaccggca atctctaccg ccagttcgac agtttgcggt acgccagccg tctcgaggat
     2521 aagctgatta aagcgcagtt agcgtaaaac gctctgcatt tgcggcgcgc tcatttcgct
     2581 actgttaaat gtttgcatcg caccgaaaga ttttgccagt gccagttttt ctgaactaat
     2641 gtcgatcgcc gtcacactct ttgctcccag cgcgacagcg cactgaatgg ccagcaggcc
     2701 aatggttccg gcaccaataa taataacgtt tttattctca caaccttgcg ctaaatgaaa
     2761 agcatgcagg ccaacggtaa tcggttcaat aaaagcccca tcctcaatag gcatatcagt
     2821 aggtagagca aagacatttt tccgcttaac gacaatatat tcggcaaatc caccatcacg
     2881 ccgtgagcca ataaaatcat attttgcgca ctgggaataa aaccctttca aacactctgg
     2941 acaagtaaaa cagggtaata acggcacaca ggcaaccgca tcgccaggat gtaaatcatc
     3001 aacaccggat cccaccgcat caatatagcc gctaaattca tggcctaacg ttattggata
     3061 ataatgtgca ccatttttaa atatcctggg taaatcggaa ccacataagc ccgagctggc
     3121 aatttttacc cgcacctcat cctgatgttt aatttcagga atgacgcttt ctgcaacgcg
     3181 cacgatacca tcagtatcat tcaccactga tttcataaaa actcctgatt gttaaggggg
     3241 ataacctccc cctcaggtaa aaattattct gcgagaacga ctttctcttg tttaataaag
     3301 ccacgcgctc tacgccaggt catgaaaata ccggtaagat aaattgcgcc gataataatg
     3361 aatccgggaa tattttgcgg ggagaaaacc tgaatcagta accaggtaat gggagaaccg
     3421 ccctgatcca ttgaagccac catacccccg gcttttaacg ccccagcatt agccgccagt
     3481 tgggtgtgca aaccaatagt ttgcgtcgcg atccacagag tgatgctcat gatgatgaca
     3541 cccgagatta aggtgcggaa cagatttcca cgatgcacgg cgactgccat cgccacaaag
     3601 aagccgatgg tggcaagatc gccaaacggc agcacctgat tacccggaac acaaacagca
     3661 attaaaatgg tgagcgggat aaaaatcagg cttgccgata ccaccgccgt atgccccagc
     3721 agtaatgcgg gatccaggcc aatcaggaac tcctgaccgc cgaacttcgc ctgtaaacga
     3781 ctacgggcct gcttagcgat gggcgttaaa ccatccatga tgggtttaat cacccgtggc
     3841 attagcagca tcactgccgc cgtttttacc gccagctgca atacaccttt gacatcgtaa
     3901 cccgcgagga tgccgataat cagccccatc acaaaaccca cggtgacagg ctcgccaaat
     3961 ggaccaaatt tgcgctgaat atcgtcggcg ctaaatttaa ttcggttaac gcctgggatt
     4021 ttctcgatga tagcatcgac cagcaccgca atcggcccca tatacgccga cgtaccgtgc
     4081 ggaatagcaa taccttccag ctcaaagaaa tttcgggtat cgcgggcaaa ccagtcgccg
     4141 agcttataaa caaacgccgc gtgaattacc acacctgcca tccctatcat ccatgaaccg
     4201 gttgccaggt gcagcaacgc gccggtgaag gtcatatgcc agatattcca gatatcaaca
     4261 tttaccaccc gcgtcatacg ggtcagtaac atcgccacgt taaccaatat cgcaatcgga
     4321 atcgccacca gcgcaatttg cgacgcccag gtcattggtg aagagcccgg ccagccgaca
     4381 tcgaccacat gcagattcag gtcgaaattt tccgccatcg ctttcgccgc cggaccaatg
     4441 gaatccagca ttaagccaat cacaaggcca atgccaacaa agccaatccc gatatgcaga
     4501 cccgctttaa agcaatcgcc tgccttcatg cctaatattt tagaaaaaat aatgatgaca
     4561 atcggaagca tcaccgtagg gccgaggtcg agaatataac gcatgacttc tgaaaacata
     4621 ggtcacccct gtaagatagt cagaatttta ttttgtaatg cttcgatacc gacaccagaa
     4681 ataaaaggca tgccgtgaac taacggaata tcgccaaaac tacgatccac tttggcagtg
     4741 gtgcatatca aatgcacacc atccatatag gtttctattt cattaacccg acactggatt
     4801 aattcaacag gaatattatg attctgacac aactctttaa tttcttccgc cgccatcgta
     4861 gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc gtttcataaa tagtcctttt
     4921 tattgttaca gagggattta ttcgtctgaa tctaaaacat actttgtgaa gtattccttt
     4981 aactgagttt caggaagagt aattagtgtc tcgacgatat cgggctgttg taacttgcca
     5041 aataaacagc gtaaaagttt caattgctgc tgcggatttt ccacaatcaa cgcaataacc
     5101 aacgacacct ccacgtcgtt atcatcatcc gcttgctgaa aatgaacttt atttgttggc
     5161 cttaacagat aaatggctga cgacttagca tgaatcgcct cacaatgcgg tatagcaatg
     5221 gcgtgctgct caagcattat cccggtaggg aattctgctt ctctggcaat taatgcctgt
     5281 ggccaggtat cataaaccac acctttcgcg agcatctcat taccgatatg ggttaaaact
     5341 tcgctacgat cgacaaaaga aattccgcta cgaacaaaca ggttagtcat ataccgtcct
     5401 tattccgcac agccgtagcg ataggcgcgc aaaacatcat aaattttatc cataatcagc
     5461 tgatgcggta ttgctgataa ttccccggac tgaatgcgtt caaattgttt gggaagatac
     5521 tgactaatca tgcccagtgg gatgtccacg ccttcaagat tcaccatcat cgtttcgacg
     5581 ctatttttaa tccgactatg cggccagtaa taacgaatac gatccgacag gctgtaacga
     5641 atatccagta atgaatcgtt aaaacccgta cgataatatt ttttccagta ttgcggttcg
     5701 tcgagcatca cttcttcaat taccgccagg caaccgctgc gattttcagg ggcgataagt
     5761 tcctgctcaa tttgtgccag tgcaaatatc gcctcgcgta aagcaaaggt taatgcggga
     5821 ccgactttca atattgcaaa gtgatcgcgg actaattccc aataagccgt ccgggtctgg
     5881 taatcggtag aatgtgcttc ataaaccatt cgggtgcttt ctatccattg cgccagcggc
     5941 tgcgcttcct gcggctgata atggataata ttgctgtgat caaattccac gcccggctgc
     6001 accacgatgg caatcacgcg tgttaacgcc tctgtcagcc cacgggcaat aaaggccttt
     6061 tgatgcgtac gtaaagtatt ggcggcatct tcaacatggg tgatgtgtac tgactgaatg
     6121 gcgctggcct caccgcccgg aaccggaact tcggtgccaa tgacatagct cagttgctca
     6181 cgctggcaat ctgtcgccac actttccgca gcaaagcaaa gcacagcagc tcgttccgca
     6241 accgtttctg gtgctaacgg tatgggatcc cccgcgcagg acattgacgc atcaagatga
     6301 attttactga aaccggcacg aacatattcc tttaccagct cgacggattt ttccatcgcc
     6361 gcatccgcat tttcttgctg ccagcagttt ggccccagat gatcgccgcc gagaataatg
     6421 cgttcgcgtg caaacccaac tttatcggca atcgtaaaaa caaattcgcg aaagtctgcc
     6481 ggtgtcattc cggtataacc gccaaattga ttgacctggt ttgacgttgc ttcaatcagc
     6541 actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt cgataaccaa cggatgggca
     6601 gaacagactg aacatatgcc gatatgttca ccagctttat gccgggcaat taacgttttc
     6661 atgttttttc cttattaaat ggcaggtgcg ttatgccctg ccctcgcagc cacaatcggc
     6721 aatcactttg ctcaccacat cgcgcattgc ggatttagcc gactgcaaat aatcccgggg
     6781 atcggtcgct tcagggtgct cggtcaggta attttttaac gcctgcgaga aggcattttt
     6841 cagctccgtt gcaacgttga ttttgcatat ccccagtttg atggtttgct gaatatcctt
     6901 agtcgataac cctgacgcgc catgcagcac cagcggtaag ttcacccact ggcgaatgtt
     6961 ctccagtcga gaaaaatcaa gcgccggtgc gctggcatac atcccatgag ccgtgccgat
     7021 ggcgaccgcc agggaatcaa ttccggttgc ctcggcaaat tcacgcgcct gagcggggtt
     7081 ggtataaaaa gcatcggctt cattgacttg cacatcatct tcctggccgc caagttgccc
     7141 cagctccgct tcgacgctga catcaaagcg atggcaaaaa tccaccacct ctttgacccg
     7201 tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc atgactgagc gcacgccaga
     7261 acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg tcgagatgaa ttgccagtgg
     7321 atggtgatat tgcttcgcca tcgcgctgac cagcgccaac agattttctg taccagcatg
     7381 agtaaatgtg ccaggcgttc cggcgatgat gaccggcgca tgcaggttgg cagcggtttc
     7441 caccaccact tgcatcgttt cgagattgtg aatattgaat gccggaaccg cataaccgcc
     7501 gcgctgtgcg ttgttcagca tctgctttgt cgataccacg tacattttca tatcctgtcg
     7561 tttgttttcg atttcaaaat ataatgaaat gatttgtttt taaatgtcga gataacgatc
     7621 acaaaagcga caatgtgaaa attgttcgag gagtaaaagg caaaaaaaaa cggcctcccg
     7681 atagggaagc cgtagcaaag tgcgcgtgtt tttatgccgg atgcggtgta aacatacgtg
     7741 gcagtgctat gtaggcatga taagacgctt ccgcgtcgca tcaggcatag gttgccggat
     7801 gcagtgtaaa cgccttatcc gtcctacggg gtggtgctgt tcgtatgagg cgtatcaggc
     7861 aatagtaatt ttgctatcga gataaacgtc ctgcacggca ttaatcagtt tcacgccgtc
     7921 agccatcgat ttcttgaacg ccttacgtcc aagaatcagc cccattccgc cagcgcgttt
     7981 gttgataacc gcagtacgca ctgcatcgct gaggtcagtt tcaccgcccg cagcaccgcc
     8041 ggagtttatc aacccagccc gacccatata gcagttagct aactgataac gcaccagatc
     8101 aatcgggttt tcgctggtca atttgctgta aacacgatcg tcggtgtaac cgtaattaat
     8161 tgctttatag ccgccgttat tttccgccat tttttgtttg acgatatctg caccgatggt
     8221 tgccgccaga tggtttgcct gaccggtcag gtcggcggaa acatggtaat caacgccatc
     8281 tttcttaaag gcggagttac gcaaataggc ccacagcact gtcaccatac ccagctcgtg
     8341 cgcacgttca aaagccgcag aaatttcttc aatctggcga cgtgactctt ccgagccaaa
     8401 atagatagtc gcaccaaccg caaccgcgcc catgttgaac gcctgctcca cgctggcata
     8461 cagcgtttga tcgtaggtat tcgggtaact tagcgtctcg ttgtgattaa gtttgacgag
     8521 gaatggaatg cgatgcgcat aacgccgcga taccgacgcc agcacgccgt aagttgacgc
     8581 cacacagtta cagcccgctt cgatcgccag ttcaacaatg tttttcgggt caaagtagag
     8641 cgggttagca gcaaatgaag ctccggcaga gtgctcaacg ccctggtcaa ccggcagaat
     8701 agaaagatat cctgtgccag ccagacgccc ggtgttgtac aacgtctgca tattacgtaa
     8761 caccgctggc gggcgattat tgtcaatcat tacgcggtct acgtagtcat gtccggggag
     8821 ataaagctgg tcagaaggaa ttgtcataca acggtgctgt aaaaggttgt cggcgtcttt
     8881 gccaagcaac tgcgcaatat ctgtcattac tatgctcccg taaattccga ttggatatcg
     8941 gctatggatt gtcctggccc gccttttgcg ggcaatcata atcctggtcg ttacgggcga
     9001 gtttttccat cttttggatg ttttttcagc gttttctgtt ggctcgattc atcagaaaaa
     9061 atgttagcgc ggtcaagttt tcaccgcaaa ggtatttaaa aggtattatt aagtggtatt
     9121 gtcatcgcgt accttacatt acctgtcatg aaggaattaa aagatgaaaa caacagcaaa
     9181 gctgtcgttc atgatgtttg ttgaatggtt tatctggggc gcgtggtttg tgccattgtg
     9241 gttgtggtta agtaaaagcg gttttagtgc cggagaaatt ggctggtcgt atgcctgcac
     9301 agccattgcg gcgatcctgt cgccaattct ggttggctcc atcactgacc gctttttctc
     9361 ggcgcaaaaa gtgctggcgg tattgatgtt cgcaggcgcg ctgctgatgt atttcgctgc
     9421 gcaacagacc acttttgccg ggttcttccc gttactgctg gcctactcgc taacctatat
     9481 gccgaccatt gcgctgacta acagtatcgc ttttgccaac gtgccggatg tggagcgtga
     9541 tttcccgcgc attcgtgtga tgggcactat cggctggatt gcctctggtc tggcatgtgg
     9601 tttcttgccg caaatgctgg ggtatgccga tatctcaccg actaacatcc cgctgctgat
     9661 taccgccgga agttctgctc tgctcggtgt gtttgcgttt ttcctgcccg acacgccgcc
     9721 aaaaagcacc ggcaaaatgg acattaaagt catgctcggc ctggatgcgc tgatcctgct
     9781 gcgcgataaa aacttcctcg tctttttctt ctgttcattc ctgtttgcga tgccactggc
     9841 gttctattac atctttgcca acggttatct gaccgaagtt ggcatgaaaa acgccaccgg
     9901 ctggatgacg ctcggccagt tctctgaaat cttctttatg ctggcattgc cgtttttcac
     9961 taaacgcttt ggtatcaaaa aggtattatt gcttggtctg gtcaccgctg cgatccgcta
    10021 tggcttcttt atttacggta gtgcggatga atatttcacc tacgcgttac tgttcctcgg
    10081 tattttgctt cacggcgtaa gttacgattt ttactacgtt accgcttaca tctatgtcga
    10141 taaaaaagcc cccgtgcata tgcgtaccgc cgcgcagggg ctgatcacgc tctgctgcca
    10201 gggcttcggc agtttgctcg gctatcgtct tggcggtgtg atgatggaaa agatgttcgc
    10261 ttatccggaa ccggtaaacg ggctgacttt caactggtcc gggatgtgga ctttcggcgc
    10321 ggtgatgatt gccattatcg ccgtgctgtt catgattttt ttccgcgaat ccgacaacga
    10381 aattacggct atcaaggtcg atgatcgcga tattgcgttg acacaagggg aagttaaatg
    10441 aaaacagaac gtattctcgg tgctctttat gggcaggcgt taggggatgc gatggggatg
    10501 ccctccgagc tttggccacg cagccgcgtt aaagcacact ttggctggat tgaccgtttt
    10561 ctgcctggac caaaggagaa taacgcggcc tgttatttta accgcgccga attcaccgac
    10621 gatacctcga tggcgctgtg tctggcggat gcgttactgg aacgtgaagg caagatcgat
    10681 ccggatctga ttgggcgtaa tattctcgac tgggcgctgc gtttcgacgc ctttaacaaa
    10741 aacgtactag gtccgacctc gaagattgcg cttaacgcca ttcgcgacgg taaacccgtt
    10801 gctgaactgg aaaacaacgg cgtgaccaac ggcgcagcga tgcgcgtctc gccattaggt
    10861 tgtttgcttc cggcgcgtga tgttgattcc tttattgatg atgtggcgct ggcgtcgagc
    10921 ccgacccata aatccgatct ggcagttgca ggtgcggtag tcatcgcatg ggcgatttct
    10981 cgtgccattg acggagaaag ctggtcagcg attgttgatt ccctgccttc aattgcgcga
    11041 catgcacagc aaaaacgcat cactaccttc agcgcctcac tggcagcacg tctggagatt
    11101 gcgctgaaaa ttgtgcgcaa tgccgacggc accgaatccg ccagcgaaca gctttaccag
    11161 gtcgttggcg caggtaccag cactattgag tccgttccgt gcgccattgc gctggttgaa
    11221 ctggcacaaa ccgacccgaa tcgctgcgcc gtcctgtgcg ctaaccttgg cggcgacaca
    11281 gacaccatcg gcgctatggc gacggcgatt tgcggcgcgt tgcatggcgt taacgctatc
    11341 gatcctgcat taaaggcgga actggatgcg gtaaatcagc ttgatttcaa ccgctatgcc
    11401 acagcgctgg cgaaatatcg tcaacaacgg gaggcggtat gagcggcgct cgattacaca
    11461 cgctgctgcc tgaattaacc tcgcgtcagc cggtgatggt cgtcggcgcg gcggtcattg
    11521 atgtgatcgc cgacgcttat gccctcccct ggcgtgggtg cgatatcgaa ctgaagcagc
    11581 agagcgttaa cgttggcggc tgcgcactga atattgccgt ggcgttaaag cgcctcggca
    11641 tcgaagcggg taatgccttg ccgctcggtc agggcgtgtg ggcggagatt attcgcaacc
    11701 ggatggcgaa agaggggtta atcagcctga tcgataacgc cgaaggcgat aacggctggt
    11761 gtctggcgct ggttgagccg gatggcgaac gcacttttat gtcattcagc ggtgtggaaa
    11821 atcagtggaa tcgccagtgg ctggcgcgat taaccgttgc gcctggcagc ctgctctatt
    11881 tttccggtta tcaactggcc tcgccctgcg gcgaattgtt agtggaatgg ctggaaaagc
    11941 tgcaagacgt gacgccgttt atcgattttg gcccacgtat cggcgatatc ccggacgcat
    12001 tactggcgcg gatcatggcc tgtcgacctt tagtatcact caatcgtcaa gaggctgaga
    12061 ttgccgccga acgttttgct ttatccgcag agataacaac acttggcaag caatggcagg
    12121 agaaatttgc cgcgccgttg atcgttcgtc tcgataaaga gggtgcatgg tatttcagca
    12181 acgacgcttc tggctgcatt ccggcatttc caacgcaagt tgtagacacc attggggcgg
    12241 gcgacagtca tgcaggtggc gtacttgccg ggctggcctc tggtctgcca ctggcggatg
    12301 ccgtattact gggcaatgca gtggcgtcgt gggttgtcgg gcatcggggc ggtgattgtg
    12361 cgccaacgcg cgaggaacta ctcctcgcac acaaaaacgt atagatcgct gcgacagtgg
    12421 ctaatgctgt actcaatagg ccgttgctgt tggtcaagcg ccacttgctt gatcaccagc
    12481 accggtattt tgctatctaa ctgaatatgt gactgaaact cggcatccgg catccgggcg
    12541 ctaacgcggg aacgcgtacg ttgtgggtaa atgtgctggc tgcggaaata gtcataaagt
    12601 gaaatcccga tggcatcaac atcatgaatt aaatgcgccg gaacccacga ttcctcaatc
    12661 gataccgctt cttcatcgac atagcgaata cgcttaagca aaaagacatc acttcctgct
    12721 tcgaccgcca gctgctgcgc gacttcttcg ggacatttca cgacacgctt gttaacccat
    12781 agcgtatcgg gctttttccc gcgcaatacc acctgctgag aaaaacctcg cgcttctttc
    12841 aacgagtatt cgaagatgtt gttgatctgc gtgccgtaac cgcgcgaacg cgtcaccaca
    12901 ccctcttctt ccagcgcctg catcgccttg cgcaccgtaa tgcgcgaaac gccggttaac
    12961 tgacttaaat cacgctcacc gggcaaaata ttgccatgct ccagcacccc gctgcgcacg
    13021 gcatttttta ccgtttcggc aaacttaata taaagcggcg tgttatccgc cgctaaaata
    13081 cgctcgttca gttgggcaat cagctgggta tgcgcttgtt ccatttgtct ttttcctgcc
    13141 ggggagtctg gtgggagtat atcactgctt cttatttaag gcgtttcttt aatcccatca
    13201 acgaatgcct gcaactcctc caccgtgcca tgaaatacat taaaatccac cggaccattg
    13261 atgccatcta cctgtccacg gtcggaatgc tgccagaagc gccaggccat gccgtcattg
    13321 tccggacgac gttgataata gtgagccacc caccacggat attcattgaa atagcccgcc
    13381 agattggtgt gataaaaaac ggctcctgag taaataatcg gcttttttcc cgtacttttt
    13441 tcgaccattt ttagccactg acttactcgt ttgcgtaatt cttttgccga taattttccc
    13501 cgttcttcta cgtccagtac cgcagggaaa tcgccttgtg agaaatccac cgtttgcaga
    13561 aataatcgtg cctgaaccgg cgctgctacc gacggggaga aataatgata cgccccgcgc
    13621 agcaggccat tttcgcggct tagttgccag ttacgcgaaa aatacgggtc caccagcttt
    13681 tcgccttccg tcgccttaat aaaagcaaac tgtaagcgga taccgttgtc gcgcattttt
    13741 gccacccgct gccagtcgat ccgctcctgc cagcgagaaa catctattcc gtgaatggtg
    13801 taactggcag gaatgcgaat agcgaaagat ttaaccgggc gataaccgta gaaatggatg
    13861 tagtctgtaa tctgaactgc tgtcgaatag aaaaaattca ccgtctgacg cggataaatt
    13921 gcaacgatgg aaattagccc aagagcgcat aatagagagg tgagtttttt tcgactggta
    13981 attcttagct gcattggttt catccctgaa tgtcagtgcc aaaggctgac aataaccaaa
    14041 gcagctatag tacggtgctc accaccaggc gtggaagtgg tgaaccggac caataccgtg
    14101 accaacttcc agcgtgtcgg cctgggctaa tgctgatgaa agccagcttt ttgcctcctg
    14161 tacggtgtca gcccagtttg tatggcgcgg gcgtagtgca gccaacgccg cagagagcgt
    14221 acaaccagtg ccgtgggtgt ttttggtcat aatgcgcggt gcggtaaacc gttgttcacc
    14281 ctcgcgggta aacagccagt ccgggctttg ctcatcatcc agatgaccac ctttcattag
    14341 cactgcgcca cagcccatcg ccaacagcga tcgcccttgt tccagcattt cctgttcggt
    14401 gcgcgcgtgt ggcgcgtcga gcaaggcggc agcttcgggc aagtttggcg ttattaatga
    14461 aacctgtggc aataatcgac tgcgcagcgt agcaaccgcc gaaggtgaaa gcagcgggtc
    14521 gccgcttttt gccagcataa cggtgtcgag taccacgttt tggatctgat aacgttgcaa
    14581 ccgttctgcc accgcttcaa caatatcggt ttccgccaac ataccgattt tagtggtgtc
    14641 gattcgcaca tcgctgaaca ccgaatcgag ctgggcggcg acaaaatcag gctcaatgcg
    14701 atacaccgac tgtacgccac gggtattttg cgccaccagt gcagtaataa ctgagcaacc
    14761 ataagcgcca agtgccgaga aggttttaag atcggcctga atccccgcac caccactggg
    14821 atcagtaccg gcaatcgtca gagcgttaat tcgtttcatg cctgcacctc ctgcgtcaat
    14881 tgccagagcg catcaaggaa atgtggaaca aaactgcctg gcccctcgct tctggcgact
    14941 gcgcgctctc cggcttgttt catccagtga caggcagatg cgacattttc cagcgtatcg
    15001 cctggtaacg cacagcaggc agcgacaacc gccgataatg cacagccagt tcctaccact
    15061 ttggtcatta acggatcgcc accgtgaatg ccaacggcgc gatggccatc ggtaacataa
    15121 tccatctcgc cagtgaccac gacgattgcg ccagtttccc gtgccagtgt ttgtgcagcg
    15181 ggtatcgcgt tagctgcggc gtcagtggta tccactcccc gtccaccatt agcaatgcca
    15241 gctaatgcca tgatttccga agcattacca cgtatggcgg ttggcttatg agacagaagt
    15301 tccagacaaa aacggcggcg ataatcgagt gcaccaaccg ctactggatc aagcgtccag
    15361 ggtgtttgag agctttttgc ttgctcaacg gcggcacaca tcgactgtgc gcgtagctgc
    15421 gtcagcgtgc caacgttaat cagcaacgca ctggcaatag ccgcaaactg actggcctct
    15481 tcggtttcga taaccatcgc tggcgatgca ccgagcgcca gcaaggtatt ggcagtaaag
    15541 gtttgcacca catcgttggt catgcagtgc acaagagggg aatgttggtg aaaaaggtgt
    15601 aacgcgtgcg cagattgcgc tgaactcagc aggtcgactt gcatagtttg ctcctgccat
    15661 aacgtgaaga agcaatgacc tggtggtccg tgacttccct acgctggcat tatccagatc
    15721 aggtgatacg ggtatttctc agccttcacg cagaagggca ccccgagtcg tttggttgcg
    15781 atgataaatc gcagaggagg atggtaatgt ccagcgcacg cgttgtaaac gaggaaaaac
    15841 ggcagtttta caatcgcgtt aataaattat ttgatatatg aatccagcac cttcagaacg
    15901 acatccagat cttcttcacg ttttagctca tccccctggt gaacgatgtg ttccgtcaga
    15961 tgacctttaa tcacttcccg catcagaccg tttaccgcgc cacggatagc agcaatctgt
    16021 tgtaaaactg cagcgcattc gtgcggttcg tcgagcattt tcttgagcgc cacgacctgg
    16081 ccctgaatct tactggcacg cgctttcagt ttctgtttat cacggattgt atgagacatg
    16141 gcaacacctg gttaacaaga atatgaaaaa tcatagcact attaatctac tggggggtag
    16201 tatcaggtac tgggggggag tagaatcaga ttgccgaatt aatactaaga attattatca
    16261 tgaccgaatt tacaactctt cttcagcaag gaaacgcctg gttcttcatc cccagcgcca
    16321 tcttacttgg tgcgcttcat ggcctggaac cagggcactc aaaaacgatg atggcggcgt
    16381 ttatcatcgc catcaaaggc accattaaac aggcggtgat gctcggactg gcagcaacta
    16441 tttcgcatac cgcagtggtc tggttgattg cctttggcgg gatggtggtc agcaagcgct
    16501 ttactgctca atcagcagaa ccgtggctcc agttgatttc cgcagtgatc attattagca
    16561 ccgcgttctg gatgttctgg cgtacctggc acggcgagcg caactggctg gagaatatgc
    16621 acgagcatga tcatgagcat catcatcacg atcacgaaga tcaccacgac catggacatc
    16681 atcaccatca cgaacatggc gagtatcagg atgctcatgc acgagcccat gctaatgaca
    16741 ttaaacgacg ctttgatggt agagaggtca ccaactggca aattttgtta tttggcttaa
    16801 ccggtggcct tatcccctgc ccggcagcaa ttaccgtgct gttgatatgc attcagttga
    16861 aagccctgac actgggcgca acactggtcg tcagtttcag cattggcctg gcgttaacgc
    16921 ttgtcaccgt aggcgttggc gcagcaatca gcgttcagca ggtcgcaaaa cgctggagcg
    16981 gatttaacac tctcgctaaa cgcgccccct atttttccag tctgttgatt ggcttagtcg
    17041 gtgtgtatat gggcgtacat ggcttcatgg gcataatgcg ataactcaat cctttaggct
    17101 tgcatgttat cttcagccca ggatgattct taaatcagct atttccgctg actctctgct
    17161 cgctaaggat gcttttaggg catcctttca tttacacttt ttacgaaatc atgggatcac
    17221 taacaaaata tcgcttgtca gttatattgt atggcaggaa agatatgcga ctgatattac
    17281 agatccccaa agtggagagt ttatgaccat taaaaataag atgttgctgg gtgcgctttt
    17341 gctggttacc agtgccgcct gggccgcacc agccaccgcg ggttcgacca atacctcggg
    17401 aatttctaag tatgagttaa gcagtttcat tgctgacttt aagcatttca aaccaggcga
    17461 taccgtccca gagatgtacc gcaccgatga gtacaacatt aagcagtggc agttacgtaa
    17521 cctgcccgcg cctgatgccg ggacgcactg gacctatatg ggtggcgcgt acgtgttgat
    17581 cagcgacacc gacggtaaaa tcattaaagc ctacgacggt gagatttttt atcatcgcta
    17641 aaaaaagccc cctcatcatg agggggaaat gcagacacct tgttattttt tattattagc
    17701 cacttgctcg tcttgcttgt tattagtcgt atttcacgtt gattaatgcg gttgcctcca
    17761 gtgcgccaga tttaactttg tttgtatcgt agacgtagta actggcagtt atcggaattg
    17821 aaaccgttgt atctgtaagt tttgactttg ccgcaatttc ttttaaattt gtgtatcgtt
    17881 tactgctgtt agtgatattt aaaagtgtga ctgacgaacc attactttca agaataaatc
    17941 ctacgccatt atcttccgca cctttcagta ttgtgttgct gttggttaac aagtcagaac
    18001 ttgataaata cacctgcatt ttccgtgatg tttgattttg ttgacctaat aaatttttac
    18061 aacgcagaat aatatcccct tgtttactat ttattgttgc tttgttacct gtggctggta
    18121 attgtgaaac gggaatactg tctaaagtaa ttaataaatc gtccggtttg caggttgtgg
    18181 ttatcgggtt gtatgacaat gtcagattaa aggaatagac acctgcattt tgtgccaatg
    18241 catttctgta gctatttaca catgcatccc atgacttgcc acctaataaa cacagtagaa
    18301 attgtactgc ggcactcccg cgtgtcgatg atgaagcact ggataatgtc gcaactccat
    18361 taatcatcac tgaaccgccg ccccccgcag aaacattcac ttttgcatta ctggccgcag
    18421 gtgtcaccgt taccgaataa ccactggtga aactggtctg attatttgat aacgtgatgt
    18481 tgtttttatt ggtccattca aaatgtaaat ttaatttaat ggtatcgtta taaagtccaa
    18541 caataatatc cgacgggctc ataggggtga tagtatttgt actcacgcat gacgaatcat
    18601 taaaattatg ctcatatgtc gccatttgtg tttgagtaag tgcaaaatcc ccctgaacaa
    18661 ccaaattgtg ctgaacagat aactcactat aacatgccgc atagctcaca ggcataacca
    18721 tcatcattac tataaataac attatacgaa tattcataac cacctgacta ttactggcaa
    18781 atatagtttc tactttcatc aatctcttta ccgaaggtga ttgtgcatga aagtccttgt
    18841 tgcttatcaa ttgctacatt aaccgatggc ggtacttcat tggtgcgaat aaataactgg
    18901 cttccctgac cgacaacgcc aatattatga ccatggatat cattgacttc ataaccaaac
    18961 attaatggtt gcccatccgc tcttaacgct tttataaacc agggcttgcg ctgatcggta
    19021 tcaaaattaa ccagtacaac cgcgccgcga taagaggcgg caattttccg gttgccacgt
    19081 aattctgctt cgctatcgct ttgcgacaca tccagcatca ggtgattttc ccgataaggt
    19141 gtcattccgt cgtataccac cactccatta cggtttgttg tgcgatattt ttgcccattg
    19201 acataagcat ctttaattcc tggcgcattc atcacagcaa acgtttcgga aagacggttc
    19261 gccagattaa cgccccccga ccaggcgaca atgccccctg aaacactggc tccagcctgt
    19321 cgataagtac tcgactgact ataactgcca ttcactgtcg caaccggcgc gttccaggtc
    19381 aaattcgccc cagctgtcgt ttcatttccc tgattctgat aactcaggtt gaccccatag
    19441 ttaaactgat cccggcttcc aacggttcct gataatcccg tattatttga ggcaaacccc
    19501 tgatcatcaa acgtcgttga gttagacata tatatttgcc gacgaggcgt cgtaacgtca
    19561 tcaccccaat caaagggaat cgatataaaa atattaaaac gtttctcttc atgatgattc
    19621 tcgtcatacg cctggcttgc cgcgaggata tagcttatcc gccgccagtt attggaataa
    19681 ctcaactgat aatccttact gctgccgcta cgcccccagt aatctcgcca taacgtactt
    19741 aaggacacag acccccagcc ttctggcaat gactggctca tattggcaga gaagctattt
    19801 ttacggccaa aatcgttctg gtaataatcg gcaatgtcat agacatcgtt ttcatcacgg
    19861 cgataattat ctttattgtt tgcccaaacg tgatcattaa atgtccggta atcacgcgat
    19921 gaataacgcc aggccgccag accaaaacgc gtcgacgttt ggctcacaaa tttgttgtag
    19981 gcaatttgat aactttgccc gtcaaacaca tcgccgttgt cttgtttact atgcgacttc
    20041 gtggcatcga cggaaatggc accaatgcgt gtattccagc cagtccccaa agtaaacgcg
    20101 taataattat tcgcgaccat cgagccacca tacagcgtca ataaattatt aaaaccatac
    20161 tgataacccg cctggacaaa atcactttgt ttgctcgccc cttcaatatg gctacgaccc
    20221 gccgcaaaat catatttcga tacgccgggt tgcagcatat ttggcactgc tgcataaggc
    20281 accagatagg tggttaccga gccgtccgcc tctttcacgc tgacatcaag atctgctcca
    20341 ccaccggcca actgcaaatc tgtaatcgcg aacgggccag gaggaacctc tttctgataa
    20401 accacaaaac cgttctgttc aatagttacc agcgcattac tctgagcaat cccctgcacc
    20461 cgtggcgtaa aattttgttt cgagttaggc aacatctgca tatcacgaaa caaccgcaca
    20521 ccgctgaagc gaacagaatc aaaaatatcg cttgatgtgt acatatcacc cacgcgaagc
    20581 gtgccgagaa gttgggcaaa tccacgttcc agatacaggg tattgctttt ccacacccct
    20641 ggattgttat ttgttttact gaaactggca tcagaatgca gttgccaccc cagtaaattt
    20701 aacccgctgt taaaacgtac atatgtactc ttactattac ccgacgcttt atagtcactg
    20761 taatactgac tcacataata agaggtataa aacgcattaa taccccgctc ccagttttcc
    20821 ggtggaacat agccactttc cagttcttcc acccaggcct gcgggacact gaaatcgaga
    20881 cgaaaaaccc cgatatccca ggtatagctc ccaccctgaa caagttgctc aaatgttaaa
    20941 cattgcttac cgctggcgaa gttatcgcta ttaatgccta accgcttgat aacttctatt
    21001 gataaacatg tttcttgcgg gttgtcttta acaataatct catatttccc gcgccattgc
    21061 ttattgacat aaatatcgat gtcatactgc cccggtaagg gttgattatc atcaagacga
    21121 atatttgcaa cctgctggtc tttcattcca cctatcataa aatgggtatc aaaggtttct
    21181 tcagctgcat aagcttcaat gccgagcaat aacgctacga ttgctgatgc aagtggggtc
    21241 attctcaaca taggcagctc ctgcaattaa attttgtcac taatatagtt gccgtggtca
    21301 tcgataatgg tcagatacca gttatttgca ttattacttt tgacattaac actctgactt
    21361 tctaaggggg caatcataat agtttcataa ttgactttaa cattattagc tttgacatcc
    21421 gaaatcgtca cccaattagc tgagtcattt tttatcacca atccattgcc actgcgattt
    21481 accagtaact ttttaaaggt cgctttattt accggagcaa tacccgctgg ccggtaaaac
    21541 aacttaattc tgttttgcat cgcaaacttc agtgcatttt tgccttcttg ctctggacta
    21601 tttggtggaa tatccagaac attcagataa aaaatgcttt ctttattagt gggcagttta
    21661 ttcggcataa ttttgatttt tacttgctgc ccggaatttg cccctatttt tgccactggt
    21721 ggcgttaaca tgaaaggaac ctgaattttt tctggtggta acgacgtatc gccatcatca
    21781 atccacgcct gcagcagcga agaacggttt ccctggttca tcaactgcac cataacttct
    21841 ttattttctg ccgggtaaat aatgcgcgtc ccgtagataa caattccggc atgtgcagga
    21901 aggaccatag aaaaaatgag taaagataat aatcctttca tcttaatgcc tcttaaatta
    21961 aaaagccgcc atggggatag cggccatgtt tattacctgg agaaacttac aattttattt
    22021 attaatcctg cgccacacgc ataaccagag aagataacac atcacctgat tgcactgagt
    22081 tatctgtcag tttcaggtat tttgctttaa acggaatagc atattgacct tccgctgttt
    22141 tataactctc aatgttaaaa ggtgcagaaa catccaggac atcgctgtta tcacctaaat
    22201 agagatagta acccacggta tcgttgctac tggctatctc cttatcgtta ccggttattt
    22261 tatcgttggt aatcaacgag agttttacac cagcaacttc tttcgggcag ctaattttta
    22321 acatgctgct ggttacaccg actgcatcat cataggtaga atatttagct ttaccttcaa
    22381 agacagtggt tggtacagtc ggcatattaa tacttactga atcaccaccc gcttcgaatt
    22441 tacacggaga ttctgcaata ttccccttaa tagttaatgt tccggtatca acgtctgcag
    22501 caaatactcc agcgctcata aaaaaagaag aaaagaca
//
LOCUS       DAZFDP010000069        22347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFDP010000069 DAZFDP010000000
VERSION     DAZFDP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(225..1049)
                     /gene="iclR"
                     /locus_tag="ACHJ1G_002727"
     CDS             complement(225..1049)
                     /gene="iclR"
                     /locus_tag="ACHJ1G_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4764560.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1249..4932
                     /gene="metH"
                     /locus_tag="ACHJ1G_002728"
     CDS             1249..4932
                     /gene="metH"
                     /locus_tag="ACHJ1G_002728"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4764561.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTTSISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5152..6783
                     /locus_tag="ACHJ1G_002729"
     CDS             5152..6783
                     /locus_tag="ACHJ1G_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4764562.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6874..7563)
                     /gene="pepE"
                     /locus_tag="ACHJ1G_002730"
     CDS             complement(6874..7563)
                     /gene="pepE"
                     /locus_tag="ACHJ1G_002730"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4764563.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7946..9175)
                     /gene="sorE"
                     /locus_tag="ACHJ1G_002731"
     CDS             complement(7946..9175)
                     /gene="sorE"
                     /locus_tag="ACHJ1G_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO4764564.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQLGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9227..10049)
                     /locus_tag="ACHJ1G_002732"
                     /pseudo
     CDS             complement(9227..10049)
                     /locus_tag="ACHJ1G_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10060..10857)
                     /locus_tag="ACHJ1G_002733"
     CDS             complement(10060..10857)
                     /locus_tag="ACHJ1G_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO4764565.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10923..11417)
                     /locus_tag="ACHJ1G_002734"
     CDS             complement(10923..11417)
                     /locus_tag="ACHJ1G_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4764566.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11417..11824)
                     /locus_tag="ACHJ1G_002735"
     CDS             complement(11417..11824)
                     /locus_tag="ACHJ1G_002735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO4764567.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11834..12640)
                     /locus_tag="ACHJ1G_002736"
     CDS             complement(11834..12640)
                     /locus_tag="ACHJ1G_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4764568.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12710..13657)
                     /locus_tag="ACHJ1G_002737"
     CDS             complement(12710..13657)
                     /locus_tag="ACHJ1G_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO4764569.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14005..14877
                     /gene="rluF"
                     /locus_tag="ACHJ1G_002738"
     CDS             14005..14877
                     /gene="rluF"
                     /locus_tag="ACHJ1G_002738"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4764570.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14878..15150)
                     /locus_tag="ACHJ1G_002739"
     CDS             complement(14878..15150)
                     /locus_tag="ACHJ1G_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4764571.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            15680..16807
                     /locus_tag="ACHJ1G_002740"
     CDS             15680..16807
                     /locus_tag="ACHJ1G_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO4764572.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            16813..17604
                     /locus_tag="ACHJ1G_002741"
     CDS             16813..17604
                     /locus_tag="ACHJ1G_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO4764573.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(17640..18566)
                     /locus_tag="ACHJ1G_002742"
     CDS             complement(17640..18566)
                     /locus_tag="ACHJ1G_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO4764574.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(18711..20060)
                     /gene="lysC"
                     /locus_tag="ACHJ1G_002743"
     CDS             complement(18711..20060)
                     /gene="lysC"
                     /locus_tag="ACHJ1G_002743"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4764575.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(20152..20348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            20585..22234
                     /gene="pgi"
                     /locus_tag="ACHJ1G_002744"
     CDS             20585..22234
                     /gene="pgi"
                     /locus_tag="ACHJ1G_002744"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4764576.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         5420 a         5696 c         5842 g         5389 t
ORIGIN      
        1 tcgctcaatc tcataatgca gccgtaaaag tttcggtgga atgagatctt gcgattttgt
       61 taatgattaa gtagtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg
      121 tatgacatta agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac
      181 tcaggtatga tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg
      241 tacgccagcg tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcgg
      301 tcatcggtaa tacgtgaaat cggtccggaa attgaaattg cggcaaacgg ttcgcggtgc
      361 tcatcgaaaa tacacgctgc aaggcaacgt agccccagtg catgttcctc atcgtcaaat
      421 gaataacccc gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccagc
      481 gttgcgtggg tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg
      541 ctcagttggg ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg
      601 ataggcgcgg acattcgcat cagatgcgta cactgtacct ggtcgataat aatcgcttcg
      661 tgatcgcttt gatcaagcac cgccatattg accgtttcgc cagactcttc cattaaattg
      721 cgcaggatag ggtgaacaat cgctaacaaa ttacggctct ggagaaagct gctgccgacc
      781 ataaaggcat gtgcgccgat tgcccaatgt cccagttcgc cgacctgacg cacgaaaccc
      841 tgctgttgca tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc
      901 gccagctctg tgagtgccac actgccattg gattcggcaa tccactccag taatttcagg
      961 ccacgcgtta aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg
     1021 ccgcgtttcg cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatc
     1081 attttcattt ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt
     1141 tgaacaaatc tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg
     1201 gtttgttgaa tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa
     1261 gtggaacaac tgcgtgcgca gttaaatgaa cgtattctgg tgctggacgg cggtatgggc
     1321 accatgatcc agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac
     1381 tggccatgcg atctcaaagg caacaacgac ctgctggtgc tcagcaaacc ggaagtgatc
     1441 gccgctatcc acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc
     1501 aactccacga ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac
     1561 tttgcggcgg cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag
     1621 aaaccgcgct acgttgccgg tgttctcggc ccaaccaacc gcacgacgtc tatttctccg
     1681 gacgtcaacg atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctatcgc
     1741 gaatccacca aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc
     1801 gacaccctta acgccaaagc ggcggttttt gcggtgaaaa cggagtttga agcgctgggc
     1861 gttgagctgc cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc
     1921 gggcagacca ccgaagcatt ttacaactca ttgcgccacg ccgaagctct gacctttggc
     1981 ctgaactgtg cgctggggcc cgatgaactg cgccagtacg tgcaggagct gtcacggatt
     2041 gcggaatgct acgtcaccgc gcacccgaac gccgggctac ccaacgcctt tggtgagtac
     2101 gatctcgacg ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggttttctc
     2161 aatatcgtcg gcggctgctg tggcaccacg ccacaacata ttgcagcgat gagtcgtgca
     2221 gtagaaggat tagcgccgcg caaactgccg gaaatccccg tagcctgccg tttgtccggc
     2281 ctggagccgc tgaacattgg cgaagatagc ctgtttgtga acgtgggtga acgcaccaac
     2341 gtcaccggtt ccgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcactg
     2401 gatgtggccc gccagcaggt ggaaaacggt gcgcagatta tcgatatcaa catggatgaa
     2461 gggatgctcg acgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg
     2521 gatatcgctc gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt
     2581 ctgaagtgta tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac
     2641 gcctttatcc atcacgcgaa gctcttgcgt cgttacggtg cggcagtggt ggtaatggcc
     2701 tttgacgaac agggacaggc cgatacccgc gcacggaaaa tcgagatttg ccgtcgggcg
     2761 tacaaaatcc tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac
     2821 atcttcgcgg ttgccaccgg cattgaagag cacaacaact acgcgcagga ctttatcggc
     2881 gcgtgtgaag acatcaaacg cgaactgccg cacgcgctaa tttccggcgg cgtatctaac
     2941 gtttctttct cgttccgtgg caacgatccg gtgcgcgaag ccattcacgc agtgttcctc
     3001 tactacgcta ttcgcaatgg catggatatg gggatcgtca atgccgggca gctggcgatt
     3061 tacgacgacc tgcccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgc
     3121 gatgatggca ctgagcgttt actggagctt gccgagaaat atcgcggcag caaaaccgac
     3181 gacaccgcta acgcccagca ggcggagtgg cgctcgtggg aagtgaataa acgtctggaa
     3241 tactcgctgg tcaaaggcat taccgagttt atcgagcagg ataccgaaga agcccgccag
     3301 caggctacgc gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc
     3361 ggcgatctgt ttggcgaagg gaaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc
     3421 atgaaacagg cggtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggcaaa
     3481 accaacggca agatggtgat cgccaccgtg aagggcgacg tccacgacat cggtaaaaat
     3541 atcgttggtg tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg
     3601 cctgcggaaa aaattctccg taccgctaaa gaagtgaatg ctgatctgat tggactttcg
     3661 gggcttatca cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag
     3721 ggcttcacca tcccgctatt gattggaggc gcgacgacct caaaagcgca cacggcggtg
     3781 aaaattgagc agaactacag cggcccgacg gtgtatgtac agaacgcatc gcgcaccgtt
     3841 ggtgtggtgg cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc
     3901 aaggagtacg aaaccgtacg tattcagcac gggcgtaaga aaccgcgcac accaccggtc
     3961 acgctggaag cggcgcgcga taacgatttc gcttttgact ggcaggctta cacgccgccg
     4021 gtggcgcacc gtctcggcgt gcaggaagtt gaagccagca tcgaaactct gcgtaattac
     4081 atcgactgga caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg
     4141 gaagatgaag tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg
     4201 gataaattaa gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca
     4261 aaccgtgtgg gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac
     4321 gtcagccacc atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct
     4381 gacttcgttg cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact
     4441 ggcgggctgg aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac
     4501 aaaatcatgg tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat
     4561 gagcgtgtgc gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa
     4621 ctgatccgcg aaaactacca gggcatccgt ccggcaccgg gctacccggc ctgcccggaa
     4681 catacggaaa aagccaccat ctgggagctg ctggaagtgg aaaaacatac cggcatgaaa
     4741 ctcacagaat ctttcgccat gtggcccggt gcatcggttt cgggttggta tttcagccac
     4801 ccggacagca agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc
     4861 cgccgtaaag gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat
     4921 gacgcggact gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag
     4981 tgccctgtct ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag
     5041 ccggaaaaac gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt
     5101 tggtcattca attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg
     5161 ctgcttcacc tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc
     5221 ggcgtaatgc gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa
     5281 aagccgctcg ccttttgcgc ggggatcggc gttaccgcac tggtgcagag cagtaatgcc
     5341 accaccatgc tggtgacctc gtttgtcgct caggatctgg tagccctcgc accggctctg
     5401 gtcattgtgc tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac
     5461 ttatcctggc tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa
     5521 cagtcacgcg ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttactggcg
     5581 ctggagttga ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc
     5641 tttgcctcgc tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt
     5701 atcagctact ccagccttgc tgctgtactg ctgactgcga ctctgaccgc cgcaggcatt
     5761 atctccttcc ccgtggcgct ctgtctggtg attggtgcta acctcggttc cggcctgctg
     5821 gcgatgctca acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg
     5881 ctgtttaagc tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca
     5941 atggggaagc tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac
     6001 cttgtacgtt gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg
     6061 attattcgcg atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc
     6121 gcgctggata cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc
     6181 gacgccatgg aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag
     6241 gagaaagagc tgcgtaagct ggcagatgat atcaacgttc tctataccgc cattaagctg
     6301 tatctggcgc ggatgccaaa agaggaactg gcagaagaag agtcgcgtcg ctgggcggag
     6361 attattgaaa tgtcgctcaa ccttgaacag gcttccgata tcgtcgagcg catgggcagc
     6421 gaaattgccg ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa
     6481 ctggatgcgc tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc
     6541 tctggcgatg tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt
     6601 aatcgccgct attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt
     6661 gaaaccagtt cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt
     6721 tgttcggtgg cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat
     6781 taatattgtg aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga
     6841 acaagatgcc ggatcatgca tccggcaatt tacttaaaaa cggtgaccag cttctagcgg
     6901 caccgcttct tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg
     6961 acctttactc actgtgatcc agttaccttc cggtagacca ataatcgtca gttctggcgc
     7021 gacgaccagc agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag
     7081 cgcgttggtg aagtgcgggt tgatttgcag cgggaacaga tttagcgcat cgaaaccttg
     7141 cggatcgaca atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc
     7201 tgcgctccag ccaatataca gcgcaccacg tttaaccacg tcagtaattg gtgccagcag
     7261 cccgcgctcg cggcactgtt tcagcaactg gaaagtattc ccgcccccga caatcacgat
     7321 ctcagcattt tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagatac
     7381 acccagcgga gcgagaaccg cagccgtttt cgctgtgtaa tcatcccagg tctgcgttac
     7441 gccagcgaaa ggaataaata ccgctgaacg gcgaccctgc aactgttcag caatcagcgg
     7501 cagcgcatgt tccagccagg ctttacccgg cagcgtcgag ttactcaata aaagcagttc
     7561 cattacttct ccagttagta agaaatcacg ggaggcatgc taccactgcg aatttaacca
     7621 cttactgaca tggctcacga tgtcacgtat tgcaccgggc gctgagactg atgacaaaca
     7681 cagcattgcc tgatgcgcta cgcttaccag gcctaccaga taactgcaat atattgaatt
     7741 ttcgagatta tgtaggccga ataaggcttt attccgcatc cggcataaac aatgtgtact
     7801 ttgccaccaa tttgagcgcc ccttaaggcg ctgatgtttt tttatccttc tcgtgcacat
     7861 tgggccgctt cagcaatcag ccacacatca ggtcgcgaag ttgccgcggc gaccagccag
     7921 cgacatgcag agttaagtag cgcgattaat catgggaaat tgcctctgca tgagtgagca
     7981 gatattgctc cgcctcaccg gaccagatcc cctgatgacg cgccagaatc gccgccagtg
     8041 cttgatcctg aatctgcgtg agtgacgtca gcggcaggta tttcccggta tacaccagct
     8101 ttttgccgcc gccgacggca ggcaattcaa gcgtggtttc gcccgcggca ttcagcccaa
     8161 gaatatgtgt taccactttt gcggcctgca cttttttctc ttcaatcagt ttgaccgccg
     8221 cgcgcatgtc gtcggtattg ccgcctgacg tgcccacgta gtgggtaaat gcataatgca
     8281 catcgtagaa attaattggc gcgctgaaat gtttatcctg cggtccggcg aagaaattca
     8341 ggcaaccatc tgtcgccagc aaggaagagg cgagagtcac cagtccttca ttaggcacaa
     8401 agacgaaaat atcatcgaag ccgtgaccgc cactcagcgc cattagcgta tcaaatgctg
     8461 catcggcggc attgagataa tgaatcagtg tttgcggttc tgatggatag tgcttgcgcg
     8521 cataactcaa tttatcgttg tcggtatcgg taatgacgag cagcgacggg ttaacgggtc
     8581 catgtagcgc ataatcaatc gccaacagtc ccattggtcc ggtgccgccg aggatcagca
     8641 tgcgcccttg cgggcgaatc cccatcgtgt ggttataact accttcctga agatgatagt
     8701 tggcgttgaa cgcgccaatc acgcaggaaa gcggttcaac cagtgagcct tcaaaatagg
     8761 tttcgccgtc gtatgccagc aggcaatctt gttccatgac ctcgttggga ataaccacat
     8821 gcgtggcttc gccgcccacc cacgggaagg agtagccggg gcagtccggg cggtcgggga
     8881 gttgcaggtt ggcctgaatc acataacgct gacccagctg gaatttgtgc tgccactttt
     8941 tacccacggc cagaatatcg ccgcaaaact cgtggccgat gatgatgggg ttggtcgcca
     9001 catcgtcggg tactttttta tggttttcac ccagattggc ctctttccag gaagagaggc
     9061 acaggctgtc agtgaccacc gtcgcgagga tttcatcctc ctgcatttca ggaaggtcaa
     9121 aggtttccag gcgtaaatca cgtttaccat aaagacgcag agctgttgtt ttcataaacc
     9181 acctcatgtt gagcgatggc cccggcagtc gtgccggggc gaagaatcag gacaggaagc
     9241 ccagcgcatt accgataatc cctaagccaa acagggcgaa aatcagccat accgggttaa
     9301 tttttttgtt gagcagacga accataagca gcgtcagacc gagcgccagc aaaccagggc
     9361 aaagttggtc gagaatgttc tgcacggtca tggtgacggt ggagccatcg gcggcatgcg
     9421 tttgtgaaac caccaacggt acgttgattg acgtccattt ggtgaccagc acgcccatca
     9481 caaacagtcc gagaatcgac gcgccttcgg tgagtttttg cagcacattc ccgcccatat
     9541 cgctgacgat attcacccct ttgcgaaagc cgagctgtag gccataccac ttcatcgcca
     9601 gacgcaccgc attgaaaata aagaaaaaaa agcgggccga ggatgttgcc agaaagtgcc
     9661 agagatgcgc cgagcgcagc ggtaatcggg cgcagcgttc cccaaaccag tggatcgcca
     9721 acccctgcca atggtcccat cagaccgact ttgatgccgt taatggtacc gtcatcaatt
     9781 tccgcgccgt tggcccgcgc ctcttccatg gcggcagtga cgccgatgac cgggccacat
     9841 acggctggcg tggtattgaa gaacaccagg tgtcgcctga gcgccgcaac ctgatcctct
     9901 tttaatgggt aaagtcgctt gatggcgggg atcatgtcgt agcaaaagcc cagcccgtga
     9961 atacgttcaa agttaaagga cgcctgttgc aggttggagc gcagaaacat gctcaccaga
    10021 tcgctgcgtg taatttttct ctgttccatg atggctccgt taatcgtcaa gttggtcaag
    10081 ggcggtggag gtggtggcct ggggttgagg ttcagcttta cgccactgtg gatttagctg
    10141 gatgtagacc agggccatga tcacgccgac gccaccgaac gccagcagac tgagatcgag
    10201 gtagccacct gcgaggaaac cgaggaaaaa gaaaggcatc aaatatttca cgcccatcat
    10261 acgtagcacc atggcgtaac cgaccaccac gataaaaccg ccagcaattt gcagaccgcg
    10321 cgtcacgaat tccggtatcg cgctcagcat attgctcacc atatcggcgc tgacgaacag
    10381 tgagacaatc agtgccggaa tagcaacgcg cagcgcctgc acgccaagcg cggagacatg
    10441 cagaatatcg agagtgcgaa accgcgcttc ttccgctgct ttatccgccg cgtgctggaa
    10501 cactacggtg atggtacggg caaacacagt cagcacctgg cctgccgcag ccacaggcaa
    10561 cgcgatggcg attccggtgg cgatgctttg ctgaccaacg ataaccagaa tggcggagat
    10621 tatgctggcg agtgcagaat ccggagattg cgccgcgccg acgttcatcc agccgagagc
    10681 tatcaattcc agcgtaccac cgagtattat tccggttttt aaatcaccga gaattaaacc
    10741 aatcaccgta caggcaatta acgggcgatg agtctgaaat tcatccagca cgctgcccat
    10801 tccggcaata caggaaaaaa gaaatatagc aatgatttgt agggtactta tttccatgat
    10861 ttttcacctt ttaagtgtcg tcatccctgc ctgttaaagc atatgtacac aggcgctatt
    10921 atttaatttg cgattaactg ttcgtttatt ttgtcgataa tattgattga gggatctgac
    10981 gctacgacgc gtaaatccag tatgacgcca agattattta actcatgaaa tgcgttaata
    11041 tcatcatcat ctaatgaaac cgctttggtt aactgttttt taccgggccg ccaggccatg
    11101 ccgccaatat tcagggtgtc tatttttacc ccctggcgaa ccatcgctaa ggcatcctgt
    11161 gggcgagtga aaagataaaa aacggtttcg tcctgatatt gcgggttgtg ataaacagcg
    11221 acggcttttt caatattgac cacgttaacc ttcattcccg gtggcgcagc ctgacgcaat
    11281 aatgtccggc gaacttcatc gttataaact tcgtcattgc agataataat gcgctgggcg
    11341 ttagccactt tcgaccagac ggtagtgacc tgaccgtgga tcaggcggtc atcaatgcgg
    11401 gcgagcgtaa tgttcatcag aaatcctcct ctgccgttgc cagttgtttc cagacaacgc
    11461 aggtttgctt tgcgatttgc gtcaggtgtt cacacagttc atcaacattc atgctgtcct
    11521 ggttatcaac cagttcgagc gccagcggca gagaaaggcc gctgattacc cgcagacgag
    11581 gattgcgcat cgcaagcatc gctgccgcat tccacgggct gccgcactgt aaatccacgg
    11641 caatcagcca ctcatcttga ttgtgaatgc ttactaactt ttccagcttt gctacgatgt
    11701 ctccggcgtt ctcgccgggc acaaattcaa cggcttcaac attggcgttg ccgtaaacca
    11761 tctgaaccga ttccagcatg gcgcaagcta atttgccgtg ggcacaaaaa atggcgttga
    11821 ccataaagcc tccttagccg cgcgttttgc cgcccgcaat gttagtggtt actccggtga
    11881 tgtagctggc gcgttcagac aacagataac aaacaaaatc agcgacttct gctaatcttc
    11941 cggcgcgccc aataggaatg gcgtttttgg tatagccttc acgcagctgc tcgacggtga
    12001 tattgcgcgt ccacgccagc gcctcttcat attccggcgt gcgtagcccg gttttttcca
    12061 gaatcccggg cgcgataccg accacacgga taccgtgttt acccagctct ttcgaccagg
    12121 agcgcgtgaa gctattaagc gcggctttgg tagcggcgta acagctttgg ccttctgagc
    12181 cttccagtcc actttctgag gaaacattca caatcacgcc atcatgttgt ttgaccatct
    12241 gtcgcgccac cgcctgcgac atcagaaaaa cgcctttctg gttgatattg accatttttt
    12301 cgaatgcagc ttcgttgagt tcatactgcc cggcaggcgc tttctcatcg accagcagac
    12361 gcgggaaatt gaccccggcg ttattgacca gaccgtcgat gcgaccaaaa cgctggataa
    12421 tttccgctac cgtatgattt acctctttgg cgctggaaat atcggtcggc caaaactgat
    12481 agcctttatg accttcatat tgaccatcgc caccgtgaat atctaccatc tgtacgtttg
    12541 cgccttgtgc taataattcc tctacaatcg ccagaccaat accggatgcg ccgccggtga
    12601 cgataatgat tttatcctgc aaatttaacc acgtttgcat aacagactcc tgattttaga
    12661 taatagggac ccgccgtaat ttttattacg gtaaacatcg ggaagatttt tatttcagta
    12721 atagttcagc tgtgctgcta ttcgttacca gacaattaat atattttcca cgcagtgcac
    12781 caataattcc gctgtatttt tcttcactca tggcaatgcc aatggaatat cgcgcctgct
    12841 ttaatttatt catttcgata gagagcgttt tttcgctcat attcgtttca accattgcgc
    12901 cgtgaatatc aaaaaagcgc gagcaaatat cgccagcaac ctggcgggca ttcaggtcgt
    12961 cactctcttc accaccataa aacgcatgcc agttagcgcc gtcgcgaatg gccggtgagc
    13021 caattcccac cagggcgaca tccagattat cccagtaggc agagatggtt ttaaagtgct
    13081 gagactgcat gatcccatta cgaattaatg ggttatccag cagagccgga aaatccgcga
    13141 gatgcgattc ccctttcagc ttcgctgccg cgctgtaggt taatgtgttt acatgatagc
    13201 ggctttcgag tttaccggac ggtccgccaa taattggtac gcagattaac tgccgcgatt
    13261 gccccgcctg cggcaagttt tcaaccagtg cgctcaccgc gcgaccccag gaaaaaccga
    13321 caatatcgcc aggctccagc aagcgatcca gcagttgcgc gccgtgtaac cccatcatcg
    13381 ccagttgagt ctcttcatcc tcatcatttc ccgacaccac cacaacgtct ttcaggccaa
    13441 acttttgctt cagttgctgc tccagccaga gattttcgtt gtagtcatag ttgatggcga
    13501 tggtgacaat tccctgatcg cggcctcgtt taagcaagcg gctgatggtg gtgcggtaaa
    13561 tacgcagttc gcgcgcgatt tgcgcctgcg tcatatcctg ttcgtaatag agttgggcaa
    13621 tcttcacaat caaacggata tcgtcactgt tttccattgc cgcctcctgg ttctgcacat
    13681 ttgtgcaaat acgctttctc accgcttgat ctgatataac cccatcatga gggcctttca
    13741 aagcgatttt tcactctttg catggggaga aatagcgctt tctcttgcac atttgtttct
    13801 ctgcgtagta gtcatcagca caggtgtgca gcgattcgta atatgttgat tatccttatt
    13861 ttgccaggca aatttgcatc gtacttccgg agacactttt gtgataaaag gcgtggcaga
    13921 tcacatttaa tttcgacgtt gccgcataac cctgtttttc cccgctaaga ggtacggtat
    13981 attccgcgtt ttacaggaga atttatgctg cccgactcat cagtccgttt aaataaatac
    14041 atcagcgaaa gcggaatttg ctcacgccgc gaagcggatc gctatatcga gcaaggcaat
    14101 gtgttcctta atggcaagcg agccaccatt ggcgatcagg tgaaacccgg cgacattgtg
    14161 aaagtaaacg gtcagttgat tgagcctcgg gaagccgaag atttggtact tatcgccctg
    14221 aacaagcccg tcggtattgt cagcaccacc gaagatggcg agcgcgataa cattgtcgat
    14281 ttcgttaacc acagcaaacg cgtgttcccg attggccgcc tggataaaga ctcccagggg
    14341 ctgattttcc tcaccaatca cggcgatctg gtgaataaga tcctccgtgc gggtaacgat
    14401 catgagaaag agtatctggt gacggtcgat aaaccgatta ccgatgaatt tattcgtggt
    14461 atgggggcgg gggtgccgat cctcgggacg gtgacgaaaa agtgcaaagt caaaaaagaa
    14521 gcgccgtttg tcttccgcat taccctggtg caggggctga accgtcagat ccggcgcatg
    14581 tgcgagcatt tcggctatga agtgaaaaag ctggaacgta cgcgcattat gaacgtcagt
    14641 ttaagcggca ttccgctggg tgagtggcgc gatttaactg acgatgagtt aatcgacctg
    14701 tttaaactca ttgaaaattc ctcttccgag gtaaaaccta aagcgaaggc taaaccgaaa
    14761 acagcgggca tcaaacgtcc agtcgttaag atggaaaaaa cggcggaaaa aggcggtcgc
    14821 ccggcgtcca acggtaagcg ttttacctcg ccggggcgta aaaagaaggg acgctaatca
    14881 gcgcctgccg cgcgtcgacg tatttgctga ccagctaaat gacgcttcag gatccggttt
    14941 ataagcctgc tttttcttca actggcgggc ttttttcgct tcagcctcac gctcgaccat
    15001 taatttatcg atgtactctt tcttaatgct gttggtttcg gagttggtca gtacgcgacc
    15061 atgcgcaatc cgcgcccgat ccagcagtgt tttaagttca cgctgctcgc gttcagtcat
    15121 ctctttttgg gtaatacggg ggagtgccat gatggtgccc tcagtgagcc agagagttca
    15181 gtgtacggga attcgtttgc gagctgaact gataatattc ctttgttctc ttcttaaata
    15241 tttatctttc ttgataattg acgataaata cagatacaaa tttagcgaaa agttttccct
    15301 ctacaaacaa aaataattta ttaaatcttt atttttcatt tggttaataa ttttttctca
    15361 attctggcac gcttctcgca atatctcgta cgcgttgaaa ctgaattaca aagggggtca
    15421 aaccccgtcg gcacaccagg aacggccagg ctgttttccg ggcgtgttcc gtcagccttt
    15481 gacgccaatt atgggttcga ttcccacatt cattcctgaa atgaatactg gttttaactt
    15541 attaaataaa aagtgaggga ttgcgcagtc agcgatcccc gataacgtcg ccgataaaga
    15601 tgccgggtaa caggcaaaag ggaattcccc tgtcgcttcg tgtctgcttc cgcgacggct
    15661 gttaagcaag gaatagataa tgattaaaaa aataagcgta agaaaagatc agctggcatt
    15721 actctccaga aacggcgatt actacaaagt gctacatgca ggcgagcatc ttttaccctg
    15781 gttaaatacg ccagaagtct tgctgattac gctcgatggc agcgaagttc ccgacgtgct
    15841 ggcggattat ttgcgccgct ttcagcctga ttgggtggag aaatattgcc tggtggcaga
    15901 cttgtcggag attgaagccg gggcgttgta tatggacggt attttgcagg agatcctgcc
    15961 gccatcaacg cgccgtttgt actggcgtgt agaggatgat ttgacactgg tgcggatgaa
    16021 tacgcagcaa gtccaggtac aaacggaggt gatgaatgcg gtattgcaac cacggcgcaa
    16081 aggggcagtt aaaggccgcg atgcaatact aacggtgcag gttccggcct ggcatgtagg
    16141 cgtgctgaaa atcgatggtg aaacgcaggc gctgttaccg ccggggctga ccgcatactg
    16201 gaaaattaac catctggttg acgctgaagt ggttgacacc cgcttgcagg tactggaagt
    16261 cagcgggcaa gaaatcttaa cgaaagataa ggtcaatctg cggattaacc tcgccgctaa
    16321 ctggcgctac agcgacgtgt tattggcatt tagccagtta acgaagccga ttgatcattt
    16381 gtatcgggaa ttacaatttg ccctgcgtga agcggtaggt acgcgtacgt tggatgaatt
    16441 gctggaagat aaacaggtca ttgatgacgt tgtcagcgaa caggtgaaat cgcgcatgtt
    16501 gccatttggt atggagattg cctcgttagg ggtgaaagat atcgtcctgc ctggcgatat
    16561 gaaaaatatc cttgcgcagc tggttgaggc ggagaaatcc gcgcaggcca atgtgattcg
    16621 ccgacgtgaa gaaacggcag cgacgcgttc gttactcaat accgctaaag tcatggaaaa
    16681 caacccggta gcgttacgct taaaagaact ggaaacgctg gagagagtgg ctgaacggat
    16741 cgataacatc tctgtatttg gcggtctgga tcaggttctg catgggttgg ttaatatcaa
    16801 agggtgagta acatgccatt aaatggagtc agtgctgcga tgcgagaaag ggtatcgcag
    16861 cagttaaaag aaattgaacg gcggtatgat gtgaaagtac tgtacgcctg tgaatcaggg
    16921 agccgcggct ggggatttgc ctcgcccgac agcgactacg atgtacggtt tttgtatgtt
    16981 cacccgctgg aatggtatct gcgagtagag tcaccgcgcg atgttattga gctgcccatc
    17041 gacgacgagt tagatgtttc cggttgggag tggcgtaagg cgctgggatt attgaaaggc
    17101 gcgaacccga cgctgataga atggctggat tctccggtgg tttatcaaca agatgaagaa
    17161 acgataacgg cgtttaaggc aatggtccca atgtggtttt ctccgctgcg tgcgcgctgg
    17221 cattactatt cgatggcgca gaaaaacttc cccggatatt tgcagggcga cgaagttcga
    17281 ctgaaaaaat acttttacgt gctgcgtccg ctgctggccg tgcgctgggt cgaagcgggt
    17341 aaaggcgtac cgccaatgcg ctttgctgag ttactggcgg gaagcgaact tgatgcgccg
    17401 ttacgtgcag aaatagacga attactggaa cgtaaacagc gcgcaggaga agcggaatat
    17461 ggtccgcgcc gtccattgtt acatgcgttt attcgtgcgg aactggcgcg tggggaaatt
    17521 ccaccgctgt taccggatag cagagaaggg gacgttaagg aactggatag cttaatgtac
    17581 cagacggtaa tgcgccgggc ataaagcccg gcgttatttc acgccatcag ctcttattat
    17641 tattgtgtat tcccgctgat ttcattctgt ataagcgttg caatatcgtc attcgttgcc
    17701 gcttgtgaac taaatatcat cttctcacgc gaatacaccg caaacttatc tgacaggttc
    17761 gaagcaaaca ccttgcgggc attttctgcc agcaaaccgg tgacgatatc atggtggttt
    17821 ttatccactt ttcgggcaat atttcccgcc tctttgtcct tactataccg ttcattggtg
    17881 ctttcccagc ttaatgccgc tgaggttgtt tttatcatga cttctgtttg gtagcctgca
    17941 tctttgagtt tttttaatgt ttgtaacggc gtcgcggcat tacgaaatgt tccttcgaca
    18001 acaatgttga gtttactgac tatcgctctc tcaattgcac gttcgaccgt ttcgccggaa
    18061 aatttcgctg tatgggtaac aaaatcatcc ccataatttt ggtagatata attaaaatca
    18121 gggtgtagaa atcgaaaatc atcaccgtta ataatcatta tattatcttt tgtctctctt
    18181 ttggctaatt caatcagaga tgatttacct gctccgggtt gcccacctaa aacaaaacca
    18241 atgggatgct gttgcggtgt gacttcatga tttgtcagac gctgccaaat agaatcaaaa
    18301 gatgctttat tttctgaatg cgtagtattg ttgaattcat gctgatattg agttcttacc
    18361 tgctgaagga gaatcttacc ttgtgtaaaa tcaacaagtt cagcgggggt agaataaaga
    18421 taatcgcgat agctgactaa tcgcttgaga gcatcgcctg aatcctgatg cgacgaaact
    18481 gagcaattat caatgatatt tgataaaaac gcgatgcact gactggctcc gtttaaaagt
    18541 gcatcttttt ccattaaagt agccattaac actcctgtta aatgatattt ttcggtgatt
    18601 ataatactgt tctacaggag tgattttgct tttcagatat tatttaatct atgttttcag
    18661 tgaggatggc gtataacaat cggtgagcgt tatacgccag tagggaaata ttactcaaac
    18721 aaattaaaat gcagcttctg caccacctgc tcggcatctt cgccgggcac caggaagcac
    18781 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
    18841 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
    18901 gccagacctt cttctacttc cacccggcac agtgcggaaa gctccatcag cagagattgc
    18961 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
    19021 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
    19081 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
    19141 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
    19201 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
    19261 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
    19321 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
    19381 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
    19441 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
    19501 ctaccgataa atccctgggt gatcactaag ccttcattga gacgtgggag cagctgcagc
    19561 gcggccagtt ccgccagcgc ggctatatct ggctctgcac gaccaaatcg gtcgttggta
    19621 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatctca
    19681 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
    19741 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
    19801 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcgg
    19861 atagcgtcga gtttttcgaa tcgctcgcca ggttccagtc cttcagctaa agcgaccagc
    19921 agattagtga taccagcaga agccgagagg acaactaaac gcacgttggc atcagaaagc
    19981 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
    20041 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
    20101 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
    20161 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
    20221 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
    20281 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
    20341 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
    20401 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
    20461 gcttcactgc gctaagggtt tacactcaac attacgctaa cggcactaaa accatcacat
    20521 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
    20581 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
    20641 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
    20701 taagttctcc gcaaccttcg gcgatcagat gctggtggat tactccaaaa accgcatcac
    20761 tgaagagacg ctggcgaaat tacaagatct ggcgaaagag tgcgatctgg cgggcgcgat
    20821 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgctg tgctgcacgt
    20881 agcgctgcgt aaccgtagca ataccccgat tttggttgat ggcaaagacg taatgccgga
    20941 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
    21001 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
    21061 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaatcacc tgaacatgca
    21121 ctttgtttct aacgtcgatg ggactcacat cgctgaagtg ctgaaaaaag taaacccgga
    21181 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
    21241 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
    21301 ctttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
    21361 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
    21421 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
    21481 gatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtac tgctggcgct
    21541 ggttggcatc tggtacaaca atttctttgg tgcggaaact gaggcgattc tgccgtatga
    21601 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
    21661 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
    21721 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
    21781 accgtgcgat ttcatcgctc cggctatcac ccataacccg ctctctgatc atcaccagaa
    21841 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
    21901 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
    21961 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgtg aaatcactcc
    22021 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
    22081 tctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacagc tggcgaaccg
    22141 tattctgcca gagctgaaag atggcaaaga aatcagcagc cacgatagtt cgaccaatgg
    22201 tctgattaac cgctataaag cgtggcgcgg ttaatcatcg tcgatatgta ggccggataa
    22261 ggcgttcacg ccgcatccgg caaccgatgc ctgatgcgac gcggtcgcgt cttatcaggc
    22321 ctacaggtcg atgccgatat gtacatc
//
LOCUS       DAZFDP010000070        22203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFDP010000070 DAZFDP010000000
VERSION     DAZFDP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..914
                     /gene="ygcG"
                     /locus_tag="ACHJ1G_002745"
     CDS             42..914
                     /gene="ygcG"
                     /locus_tag="ACHJ1G_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4764577.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(974..2272)
                     /gene="eno"
                     /locus_tag="ACHJ1G_002746"
     CDS             complement(974..2272)
                     /gene="eno"
                     /locus_tag="ACHJ1G_002746"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4764578.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2360..3997)
                     /gene="pyrG"
                     /locus_tag="ACHJ1G_002747"
     CDS             complement(2360..3997)
                     /gene="pyrG"
                     /locus_tag="ACHJ1G_002747"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4764579.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4225..5016)
                     /gene="mazG"
                     /locus_tag="ACHJ1G_002748"
     CDS             complement(4225..5016)
                     /gene="mazG"
                     /locus_tag="ACHJ1G_002748"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4764580.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5087..5422)
                     /gene="mazF"
                     /locus_tag="ACHJ1G_002749"
     CDS             complement(5087..5422)
                     /gene="mazF"
                     /locus_tag="ACHJ1G_002749"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4764581.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5422..5670)
                     /gene="mazE"
                     /locus_tag="ACHJ1G_002750"
     CDS             complement(5422..5670)
                     /gene="mazE"
                     /locus_tag="ACHJ1G_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4764582.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5748..7982)
                     /gene="relA"
                     /locus_tag="ACHJ1G_002751"
     CDS             complement(5748..7982)
                     /gene="relA"
                     /locus_tag="ACHJ1G_002751"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4764583.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8030..9331)
                     /gene="rlmD"
                     /locus_tag="ACHJ1G_002752"
     CDS             complement(8030..9331)
                     /gene="rlmD"
                     /locus_tag="ACHJ1G_002752"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4764584.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9388..12144
                     /gene="barA"
                     /locus_tag="ACHJ1G_002753"
     CDS             9388..12144
                     /gene="barA"
                     /locus_tag="ACHJ1G_002753"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4764585.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12376..13716)
                     /gene="gudD"
                     /locus_tag="ACHJ1G_002754"
     CDS             complement(12376..13716)
                     /gene="gudD"
                     /locus_tag="ACHJ1G_002754"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4764586.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13737..15077)
                     /gene="gudX"
                     /locus_tag="ACHJ1G_002755"
     CDS             complement(13737..15077)
                     /gene="gudX"
                     /locus_tag="ACHJ1G_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO4764587.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15079..16431)
                     /gene="gudP"
                     /locus_tag="ACHJ1G_002756"
     CDS             complement(15079..16431)
                     /gene="gudP"
                     /locus_tag="ACHJ1G_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4764588.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16866..17315)
                     /gene="yqcA"
                     /locus_tag="ACHJ1G_002757"
     CDS             complement(16866..17315)
                     /gene="yqcA"
                     /locus_tag="ACHJ1G_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4764589.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17333..18115)
                     /gene="truC"
                     /locus_tag="ACHJ1G_002758"
     CDS             complement(17333..18115)
                     /gene="truC"
                     /locus_tag="ACHJ1G_002758"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4764590.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18115..18444)
                     /gene="yqcC"
                     /locus_tag="ACHJ1G_002759"
     CDS             complement(18115..18444)
                     /gene="yqcC"
                     /locus_tag="ACHJ1G_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4764591.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19066..19611)
                     /gene="syd"
                     /locus_tag="ACHJ1G_002760"
     CDS             complement(19066..19611)
                     /gene="syd"
                     /locus_tag="ACHJ1G_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4764592.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19679..20527
                     /gene="queF"
                     /locus_tag="ACHJ1G_002761"
     CDS             19679..20527
                     /gene="queF"
                     /locus_tag="ACHJ1G_002761"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4764593.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20639..22003
                     /gene="ppnN"
                     /locus_tag="ACHJ1G_002762"
     CDS             20639..22003
                     /gene="ppnN"
                     /locus_tag="ACHJ1G_002762"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4764594.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5454 a         5918 c         5256 g         5575 t
ORIGIN      
        1 cgccgtcaat ttatttatcc agatttaatg agaaatttat aatgcgctat ttcatactaa
       61 tgttcacttt cgtgtgttcc tttgttgcag cccaaccaac tattgtccca caattacagc
      121 aacaagttac ggatcttacg agtagcttaa actcacaaga aaagaaagaa ctgacgcaca
      181 agttagaatc tattttcaat aatacccaag tgcaaattgc tgtattaatt gttcccacaa
      241 ccaaagacga gacaattgaa caatatgcta caagagtttt tgacaattgg cgtttaggag
      301 atgccaaacg taatgatggg atactgatca ttgttgcctg gtcagatcgt actgtccgca
      361 tccaggtagg ttatgggctg gaagaaaagg taaccgatgc tctggctgga gatatcatcc
      421 gtagcaacat gatacccgca tttaaacaac aaaaatttgc tcagggatta gagctagcta
      481 taaacgcttt gaataaccaa ctcacttcac aacaccaata tccgactaat ccttcagaaa
      541 gtgaatcagc gtcttccagt gatcattatt actttgctat tttttgggta tttgcagtga
      601 tgttcttccc tttctggttt tttcatcaag gcagtaattt ttgtcgcgca tgtaaaagtg
      661 gagtctatat ttcagcaatc tatcttttag atttattcct gttctccgat aaaatttttt
      721 ccattgctgt attttccttc ttttttactt tcaccatatt tatggtcttt acctgtttat
      781 gtgtacttca gaaaagagca tcaggtaaaa gttatcattc agacaacagc ggttccggtg
      841 gaggctcaag tggcggcgga tttagcggcg gtggcggttc ttctggaggt ggcggcgcat
      901 ccggccgctg gtaactcaac cataaaaaat gccagcccgg aggctggcat ttttaaatca
      961 gataaagtca gtcttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
     1021 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
     1081 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccaggtca gcgatggtag
     1141 cgtcttcagt ttcgccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1201 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1261 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1321 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1381 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1441 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1501 atttaccgtc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1561 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1621 aacccaggtt cggcgcatag ccaccttcgt caccaactgc agtgttcatg cctttcgctt
     1681 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1741 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1801 caccaccgtt gatgatgttc atcatcggaa ccggcataga gtatttgccc ggagtaccgt
     1861 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1921 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1981 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     2041 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     2101 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2161 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2221 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2281 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt
     2341 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2401 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2461 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2521 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2581 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca
     2641 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2701 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2761 caaccgggta cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa
     2821 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2881 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2941 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     3001 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     3061 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3121 cgatggtgac ttcacttacc gggttcgctt cttcgaagat aacctgttcc cattcggaca
     3181 gattcgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3241 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3301 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccgga acagcgcgat
     3361 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3421 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3481 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaatc gcttcgagga
     3541 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3601 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3661 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3721 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3781 ggtccaggtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3841 tcatagtacc tggatcgacg ttgatgtacg gatccagttt catgatggtc acattgaggc
     3901 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3961 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     4021 aaaagacgat ggaataacca ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     4081 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4141 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4201 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4261 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4321 acgctccact tcgcgaaaac gacgctcaaa tttttcgttc gctttttgca atgcgatttc
     4381 cgcttttgtc cctaaatgac gagccagatt gaccgtggca aacagcaggt cccccatttc
     4441 ctcctccagt ttagcctggt cgacaacagc ctgccgcgct tcgtacatca cctcgtcgat
     4501 ctcttcgtag actttatcga ctaccggacc aagcgtcgtc caatcgaagc caacgttggc
     4561 gcaacgtttc tggatttttt gcgcacgcat taaagccggt aaactacgag gaatatcgtc
     4621 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4681 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4741 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4801 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4861 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4921 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4981 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     5041 ctgtcaggtg gaaacctgtg accagaatag aagtgagtta gtaacactac ccaatcagta
     5101 cgttaatttt ggctttaatg agttgtaatt cctctggggc aactgttcct ttcttcgttg
     5161 ctcctcttgc ccgccaggcg atacttttta cctgatcagc taacgctacg ccatcacgtt
     5221 cctgaccgga taaaacaact tcgaacggat atccttttga ttgcgttgta taaggaacac
     5281 acagacacat acctgttttg ttgttgtaca tgaaaggact caggacaaca gctggacgat
     5341 gtccagcttg ctcgctacct tttgtcgggt caaaatcaac ccaaatcaga tcgcccatat
     5401 cgggtacgta tcggcttacc attaccagac ttccttatct ttcggctctc cccagtcgat
     5461 attctcgtgg aggttttccg gcgtgatgtc gttgaccagt tcagcaagcg taaatacggg
     5521 ctctttacgc actggctcaa taattaattt gccatccacc aggtcaatct tcacttcatc
     5581 atcaatattc agattgagcg cctgcattaa cgtagccggg atccgcaccg ccggtgaatt
     5641 tccccaacgc tttacgctac tgtggatcat aaccctttcc tcaaaccgct atcatatgta
     5701 gatacagtat atatcaatct acattgtaga tacgagcaaa tttcggccta actcccgtgc
     5761 aaccgacgcg cgtcgataac atccggcacc tggttgagtt tacccagcac gcgccccagc
     5821 acttgcaggt tgtaaatctc aatggtcatg tcgatggtcg ccagttgctg tttggtgtcg
     5881 ctacggctgg caacgccaag cacgttcacc ttctcgttgg cgagaatggt cgtgatatca
     5941 cgtaacaacc cactacgatc attagccacc acgcggacca ccagcgaata tccggcggag
     6001 tagctctcac cccataccgc gtcaacaatg cgttctggcg catgggagcg cagttccgcc
     6061 agttgttcgc aatcggcgcg gtgtactgaa ataccgcgcc cctgggtaat gaagccgaca
     6121 atctcatctc caggaatcgg ctggcagcag cgcgcgatgt ggtgcatcag gttgccaaca
     6181 ccttcgacca ccacgcgacc gttatcttta ctgcggtttt gcggcgtgta gcttttttgc
     6241 tgaagttgct tcagcgcggc ggcgtcctgc tcttcggcac tcggcttatt aaattgcgat
     6301 tgcaggaagt tcaccatctg attgagacgg atatccccgc caccaatcgc cgccagcaac
     6361 tcgtcgacat cattaaagtt gtaacgcggc agcagatgtt tttctgcttc tttcaggctg
     6421 atccccagat gttccagctc gtcgtcaagg atttgccgcc cagccagaat gtttttgtca
     6481 cggtcctgtt tacggaacca ggcgtgaatt ttcgaacgcc cacggctggt tgtgacgtaa
     6541 ccgaggtttg ggtttaacca gtcacggctg gggttcggct gtttctgggt gataatttca
     6601 atctggtcgc ccatctgcag ctggtaggtg aacggcacaa tgcgcccgcc aatttttgcc
     6661 ccgatgcagc ggtgtccgac atcactgtgg atgtggtaag cgaagtccag cggcgttgat
     6721 cccgcaggca aatcaacgac atcacctttc ggcgtaaaga catacacccg gtcgtcaaag
     6781 acctggctgc gtacttcgtc gagcatttcg ccggaatcag ccatctcttc ctgccacgca
     6841 atcagtttac gcagccaggc aatccggtct tcatgtcccg aacgtgcgcc gccagctgcc
     6901 gcgccctctt tatatttcca gtgcgcagca acacccaact ctgcatcttc atgcatctgt
     6961 ttggtgcgga tttggatctc aacggttttg ccacctggcc ccagaaccac ggtatgaata
     7021 gactgataac cgtttggttt cgggttagcg acataatcgt caaactcatc cggcaggtgg
     7081 cgatagtgag tgtgcactat ccccagtgcg gcataacaat cctgtaaacg ctcggcgaca
     7141 atacgtaccg cacgcacatc aaacagctca tcgaaggcga ggttcttttt ctgcattttg
     7201 cgccagatac tgtagatgtg tttcggacga ccatacactt cagctttaac gccttcagct
     7261 ttcatctcag cgcgcagatg gccgacaaac tcttcgatat agtgttcgcg gtcgagacgg
     7321 cgttcatgca gcagttttgc gatgcgtttg tattcggtcg ggtggagata acggaagcag
     7381 taatcttcca gctcccattt cagttgccca atcccaagac ggttcgccaa cggcgcgtag
     7441 atattggtgc actcttttgc cgccagtacg cgttcatctt ccggcgcatc ttttacttcg
     7501 cgcagatgag caatacgctc cgccagtttg atgaccacgc agcgaaaatc atcgaccatc
     7561 gccaataaca tccggcgaac gttatcgacc tgttcggagg aaacagaatc agtgtgcgtc
     7621 gctttcagct ggcggatcgc cgccatatca cgcacgccgt gaataaggtt aacgaccgac
     7681 ttaccgacgc tctcacgcag cacatcttcg ctgactacgt tggcatcagc cagagggaac
     7741 agcagcgccg cccgcagcgt gtcaatgtcc atacttaatg tcgagaggat ctccaccatc
     7801 tcaacaccac gccacaataa cagactggca tccggatgcc cctgcgtctg ttgcagacaa
     7861 tacgcccagg tttcggctaa gcactcacac gacttctggc tggtaatacc cagacttgcg
     7921 atccattttt ccggatcaaa ttcaccagcc ttattgatat gtgcacttct taccgcaacc
     7981 attgtcctct cctttaggga ccagacctgc cgaaatcggc aaatcgtaac tatttaacgc
     8041 gcgagaaaag taccatcgat tccagatgtc ccgtgtgtgg gaacatatcc agcatcgcca
     8101 gtcgcgcaat ggtatatcct gcttttaata acgcttcgct atcccgagcc agcgttgcag
     8161 ggttacagga tacataaact atacgaatag gttccagttt tataatttgc tgcataacac
     8221 ctgcggcacc tgctcgcgcc gggtccagca acactttatc gaagccgttt ttcgcccacg
     8281 gctgctttgt gacatcttct tcaagatttt cgtgataaaa cgtcacattc tgtaaaccat
     8341 taagacgcgc attctgctgg cctttttcca ccagcgccgg aacaccttct acaccgacca
     8401 cactggctgc ttgtttcgcc aatggcagtg taaagttgcc cataccgcag aacagatcca
     8461 gtacgcgatc ttcaggttgc acatccagcc attccaacgc acgcgctacc attttttggt
     8521 tcacacccgc attgacctga ataaaatcgc gcgggctaaa agttaagcgc aacccgtttg
     8581 agtcatacca gggcatctca ccagagacgg tttcgagtat ctcactatcg ggggcgagat
     8641 acagatccag gccttcagaa tgcgaaaagc gttccagttt ttcgcgatct gccgaactta
     8701 gcggtgcggt atggcgcaaa atcatcagcg tgccgctggt tgcctgtacc agttcaacat
     8761 gaccaagatg gcgcataact tgtaagctgc ccaggcatgc cctgactttg ggcagcaatg
     8821 cttcaagttg gggcactaaa atggggcatt gtttaacgtc gacaatgtca ctggagcccg
     8881 ctttgcgaaa ccccatctga agttgctgtg ttttcggtaa gtagttcaga cttaaacgcg
     8941 cgcggcggcg atagccccag ggaacatcgg cgatcacttc agagacatcg tgtttcatta
     9001 atcgggcgag tgccgcactt ttgcttcgct gctgtaaatc cacgctggcg tgttgttgct
     9061 gacagccgcc gcatacgcca aaatgaggac agcgtggtgt ttcgcgttcc gggctatcgc
     9121 ttaaccggcg tacgacttta gcgcgggcat actgtttttt atcttcagta acagcaactt
     9181 ccgcgttttc ctgcggcaat aatccgggga taaataacgt tttgccgtta tgtcgcgcca
     9241 cgccctgacc aaaagagtcg aggtcgttga ctgaaacggt tatgatctga cgcgtcgtcg
     9301 tgcgtcgttt tgcagagtag aattgcgcca ttggcgagac tttctcaatt taacagtgtg
     9361 accttaattg tcccataacg gaactccatg accaactaca gcctgcgcgc acgcatgatg
     9421 attctgatcc tggcaccgac cgtccttatt ggtttattgc tgagtatctt tttcgtcgtg
     9481 catcgctata acgacttgca gcgtcaactg gaagatgccg gtgccagcat tattgagccg
     9541 cttgcagttt ctactgaata tggcatgagc ctgcaaaatc gcgaatctat cggtcagtta
     9601 ataagcgtac tgcatcgtcg ccattccgat attgttcgcg cgatttcggt ttatgatgaa
     9661 aataaccgac tctttgtcac ctccaatttt catcttgatc cctcatcaat gcagctcggc
     9721 agcaacgtgc cgtttcctcg ccagctcact gtcactcgtg acggcgatat tatgatcctc
     9781 cgcacgccga ttatttctga gagttactcc cccgacgaat cgcccagtag cgatgccaaa
     9841 aatagtcaaa atatgttggg atatattgcg ctggagctgg atcttaaatc ggttcgcttg
     9901 cagcaatata aagagatctt tatttccagc gtgatgatgc tgttttgtat cggtattgcg
     9961 cttatttttg gctggcgctt aatgcgcgat gtaaccggtc cgattcgcaa catggtgaat
    10021 accgtcgacc gcatccgtcg cgggcaactc gacagccggg tggaaggatt tatgctcggc
    10081 gagctggata tgctgaaaaa cggtatcaac tcgatggcaa tgtcgctggc tgcttatcac
    10141 gaagagatgc agcacaatat cgaccaggcg acgtccgatc tgcgtgaaac gctggagcag
    10201 atggaaattc agaacgttga gttagatctg gcgaaaaagc gcgcccagga agcggcgcgt
    10261 attaaatccg agtttctggc aaatatgtca cacgagctgc gtacaccact gaatggtgtt
    10321 attggcttta cccgcctgac gctgaaaaca gaattaacac caacgcagcg cgatcacctg
    10381 aatacgattg aacgttcggc aaataatttg ctggcaatta ttaatgatgt tctcgacttc
    10441 tcgaaactgg aagcaggtaa gctgattctg gaaagtattc cattcccact acgcagcacg
    10501 ctggatgaag tcgttactct gctggcacat tcttctcacg ataaagggtt ggaactgacg
    10561 ctcaatatta aaagcgacgt gcctgataac gtgatcggcg acccactgcg attacagcaa
    10621 atcatcacta acctggtggg gaatgcaatt aaattcaccg agaatggcaa cattgatatt
    10681 ctggtagaaa aacgtgcgct gagtaatacc aaagtgcaga ttgaagtgca gattcgggat
    10741 accggcattg gtattcctga acgcgatcaa tcgcgcttat tccaggcctt ccgacaggct
    10801 gatgccagta tttcccgccg tcatggtggc accggtctgg ggctggtgat tacacaaaaa
    10861 ctggttaatg aaatgggcgg cgatatttcg ttccatagcc agccgaatcg cggttcaact
    10921 ttctggttcc acattaatct cgatctgaac ccgaacatta ttatcgaagg gccatccacc
    10981 cagtgcctcg ccggtaagcg cctggcctat gtcgaaccaa actccgcagc agcgcaatgc
    11041 acgctggata ttttaagtga aacgccgctg gaagtggttt atagcccaac gttctccgcg
    11101 ctgcctcccg cgcattacga catgatgttg ttaggcatcg cggtgacctt ccgcgagccg
    11161 ctaacaatgc aacatgagcg attagcgaaa gcggtatcga tgaccgattt cctgatgctg
    11221 gcacttcctt gccatgcaca agtcaatgca gaaaaactca agcaagatgg tatcggcgcg
    11281 tgtctgctga aaccattaac acctacgcgc ctgttgcctg ccctgacgga attttgtcat
    11341 cacaaacaaa acacgctttt gcctgtaacc gatgaaagca agctggcaat gacagtcatg
    11401 gcagttgatg acaaccccgc taacctgaaa cttatcggcg cattgctgga agatatggtg
    11461 caacatgtgg aactttgcga tagcgggcat caggcggttg aacgggcgaa acagatgccg
    11521 ttcgatttga tcttaatgga tattcaaatg cctgacatgg atggcattcg ggcctgcgag
    11581 ctcatccacc agctccccca tcagcaacaa acgccggtta tcgcggtaac ggcgcatgca
    11641 atggccgggc aaaaagagaa gctgcttggc gcagggatga gcgattatct ggcgaaaccg
    11701 attgaagaag agcgattgca taatttgttg ttgcgctaca agcctggcag cggtatttcc
    11761 tctcgcgtcg tgacgcccga agtcaacgaa attgtggtga acccgaatgc gaccctcgac
    11821 tggcaactgg cactacgcca ggcagcagga aaaaccgatt tagcgcgcga tatgctgcaa
    11881 atgttactcg atttcctgcc tgaagttcgc aacaaagttg aggaacagct ggttggagaa
    11941 aacccggaag gcctggttga tttgattcat aaactgcatg gcagttgcgg ctatagcggt
    12001 gtgccgcgta tgaagaatct ctgccaactg atcgaacaac agctacgtag cggtactaaa
    12061 gaagaagatt tggagccgga gctgctggaa ctgttggacg agatggataa tgtagcgcgc
    12121 gaagccagca aaattctcgg gtaacggacg tcttgtcgag tagcaatcgg ctttttatga
    12181 cgctggtttc agattggtga caaagtgcct gtgtttatgc cggatgcggc gttaacgcct
    12241 tatccggcct acaaaaccaa ttaaattcaa tgaattaaaa aatgatgtag acctgataca
    12301 tagcacaaca ggctattttg cgtttagcat cagtctcaag ccggctccag atagagccgg
    12361 ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg ttatcgaacg tccagccagg
    12421 aatcagatac tgcattccca tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag
    12481 ctcatgggct ttcatcactt gatccatatc gatttctaca cccagccccg gtttttccgg
    12541 cacctgaacc agaccgcctt tgatctcaaa cggttctttg gtcaggcgct gattaccttc
    12601 ctgccagatc cagtgcgtat caatagcagt aattttaccc ggtgcagcgg cggcaacatg
    12661 ggtaaacatc gccagggaaa tatcgaagtg gttgtttgag tgtgaacccc aggtcaggcc
    12721 aaattcatgg cacatttgcg ccacacgtac cgaaccttgc atcgtccaga aatgcggatc
    12781 cgccagcggg atatcaacgg attgcaggga gagcgtatgg cccatctgcc gccagtcggt
    12841 ggcgatcata ttggttgcgg tcggcaggcc agttgcgcga cggaactcag ccatcacttc
    12901 acgcccggag aaaccttgct ccgcaccaca cggatcttct gcataagcca gcgacccttt
    12961 caggtattta ccaattttaa tcgcttcgtt cagcgaccag gcaccgttag gatcgagcgt
    13021 aatacgcgcc tgcgggaagc gttgcgccag tgccacaata gactcggcct cttcttcccc
    13081 ggccagtaca ccgcctttca gtttgaaatc gttgaagcca tatttttcat acgccgcttc
    13141 cgccaggcgc accaccgcat ccggcgtcat cgcttcttca tgacgcaggc gataccagtc
    13201 gcatgagtca tccggctggc tttgatacgg cagcggtgtg gctttgcgat caccgacgaa
    13261 gaacagataa ccgagcattt cgacttcgct acgctgttga ccatcgccca gcagcgatgc
    13321 cacgtttacc cccagatgct gccccagcag atccagcatt gccgcttcta tcccggtaac
    13381 tacatgaata gtggtacgca aatcaaatgt ctgcaaaccg cgcccaccag catcacgatc
    13441 ggcaaaagta ttacgcacca gcgtcagaac gtttttgtat ttacccagcg ttttacctac
    13501 caccagcgga atcgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc
    13561 tacgccagtg tgaccagaat tatctttgat aatcacaata ttacgcgtaa agaacggtgc
    13621 gtgtgcacca ctcagattca tcagcatact gtcatgaccc gccaccggga tgacctgcat
    13681 ttcagtaaca acaggcgtcg taaattgaga actcatattt atgtcctttt tcagaatcaa
    13741 tgacggccga aaacgggacg tttacggtca aaggtccagc cggggatcag gtactgcatc
    13801 ggacctgcgt cgttacgcgc accgccaggc agacgtttat aggcctcatg tgccttttgt
    13861 acctgttccc agtccagttc cacgcccaga ccgggcgcat caggaacggc aatttttccg
    13921 tttttaatct ccagcggatt tttggtcagg cgacaatcgc cctcctgcca aatccagtgg
    13981 gtatcgatag cggtaggatt acccggtgcc gccgcgccca catgggtaaa catcgccaga
    14041 gagatatcga aatggttatt agaatggcag ccccaggtca gcccccagtc gtcgcaaagc
    14101 tgcgccacac ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct
    14161 accgcattga gcatcaccgc atgacccatt tcgcgccagt tggtggcgat catgttagtc
    14221 gcgacgggca agccggtcgc ccgccgaaac tccgccatca cttcacgacc ggagaaaccc
    14281 tgttctgcgc cgcatggatc ttcggcatag gtaagaacat cattcagccc tttgcataga
    14341 gaaatggctt catcaagtag ccaggcaccg ttgggatcaa cggtaatccg cgcatcaggg
    14401 aagcgtttct tcaatgcccg aacagtgtcg atttcttgct cgccaggtaa caccccgccc
    14461 ttaagtttga aatctttaaa gccatagcga tcctgtgagg cttccgccag acgcacaacg
    14521 gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc actcatggtt gcccggcgta
    14581 ttttccagat aaggaagatc ggttttggtc cgatcaccga tataaaacag ataaccgagg
    14641 acggtaatag tctcgcgttg cttgcctggc cccaacagtt cgcagaccgg aacattcagc
    14701 gccttaccta gcaggtcaag caaggcggct tccagcgccg ccacggcatt aacgcgcaat
    14761 tcaaaagtcc aggcaccttt gccgaaggta tcaaaatcgg ctgcctgatt acctttatgc
    14821 acctgctgaa ccactttatt caggcgcgca acttcctggc ccagaaccat cggaatagca
    14881 tcgaccagtg tttgataaat cacctctccg cccggcgctt caccaatgcc ggtatgcccg
    14941 gcgttatcgg tgagtaccac aatattgcgg gtgaaatatg cgttatgtgc cccaccaata
    15001 ttaagcaaca tgctatcgtg cccggcgacc ggaatgactt tcatatcagt aataacagga
    15061 ctggattgtg tcgtcatctt attgccccgc gacaggtttc agctcgatac gcttgatatc
    15121 gcccaccagc accaggtagc tcagtaccgc gattaaggca tgaacaccaa cataaatcag
    15181 cgccccatta aaggagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat
    15241 gccagaaatg ttaccgaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg
    15301 cgcggtatct gccattactg cccaacccag cgccccgatg cccttaccga agaaggccag
    15361 cgccataaag ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat
    15421 cgacagcaac atgcccatta cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg
    15481 gcgcatcagc cagtcggaaa taatcccacc cagcacaccg ccgataaaac cgcaaaccgc
    15541 cggaacggaa gccacaaagc ccgctttcag aatcgacatc ccacgcgcct gcaccagata
    15601 aaccggaaac caggtaatga aaaagtaagt cagggcgttg atacagtact gaccgatata
    15661 aacgccgatc atcatccgtg acccgagcag ttgtttgatc tgcccccact tcacgctgaa
    15721 cggcacttta actttggtgt tttgctgatc catattgatc agcgcgccac ccgcggcgat
    15781 gtactccagc tctttctgat ttacccccgg atgttgattt ggctcgtgga tgactttcaa
    15841 ccagataaag ctgatgacaa tccccagacc gcccataaag aagaagacgt gtgaccagcc
    15901 cacttcatgc gtcagccagc ccataatcgg cgcgaagatc accgttgcga agtattgagc
    15961 ggagttaaaa atcgacaccg ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat
    16021 gcgactgttg ccggggaaag atggcgcttc agcaagcccg accaggaagc gcagcgtaaa
    16081 cagggcaacg ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa
    16141 catcgaccag ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa
    16201 ccaaccacca gggatctgcc cgataacata agcccatgag aaggcagaga acacatagcc
    16261 cattcccacg ggatcaaggc cgatatcttt ggccatttcc gaaccagcaa tagaaagcgt
    16321 agcgcggtcg ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg
    16381 agcattcgtg cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact
    16441 cctgaaaatt cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg
    16501 tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat
    16561 atctggcaat agcagtataa aaagcgcgcc atagcggctc accgtgcaac aacacaacat
    16621 taatgcgttc aatgaggccc gattttggca ttagccctgg acggtggaat ccacttcacg
    16681 gaaatgaaaa caagaacaag aaaggaaggg taaaaacgaa gaaataaaga agagtatgaa
    16741 atggatcgct tgactccagg caaacgccag taaaaatccg cgctatgaag cagtttttac
    16801 tggcatttgc ctgaaaagat tcgattcagc accgctaaaa cgacatttac cgctcgctga
    16861 acatatcagg acaacagcgt gccccactgt tcgacccacg gatttgattc cgtttccggt
    16921 tccgggttct cactggcgtc aatcaacagt atttcgccaa cccgctgagc gctctgttcc
    16981 tgcaacaagg catcgaactg tttgccgcca ttgcagaaat ttacataact gctgtcgccg
    17041 agcgcaatca cgccataacg cagattcggc tggaagccca gactatcttt gattccctga
    17101 aagagcggca caatgctatc aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga
    17161 acatacttat cctgataggg cagccagtcg cttaattcag gatcttcaaa taccgttgct
    17221 ttgtggccct gtgcggtcag aattgcttct gcttcttccg ccactaacag cgaatttccg
    17281 tacatggtgc cgacaaaaat accaatttcc gccataccgt tttctccctg gattaggaac
    17341 ttatctctcc atcctgaccc gatggcgcac tgaactcaac cctttcattt tcaggaagca
    17401 gaccgcgcca gccaaattgt gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa
    17461 tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa ctgactggca tgtagcatta
    17521 accgctggag gccaaaatgc tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc
    17581 tatcgccaat aatcggatga cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc
    17641 cggttttcgg ctccagttcc accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta
    17701 ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac tgctggctgc gagcctttat
    17761 cttcgcgggc aaatttatca gcgattttgt ccagttcttc caccagtgga taatccagaa
    17821 ctgcttcttc catcaaccag ccgcgcacaa tcgcatggta acgtttctgg atttggtgct
    17881 gttcaaactg ttgtgccagc agccgtccgg cctcgctgga taatcccatc aacaacacac
    17941 cagaagtggg tcggtccaga cgatgagcgg taaaaacgtg ctggccaatc tgatcacgca
    18001 cggtttgcat gaccactact ttctcgtcgc gatccagcca gctgcggtga accagccagc
    18061 cggagggttt atttaccgca accagccatt catcctgata gagtatttcc agcattagct
    18121 cgcatcatcc gcaaaaagag catccagttt ttccagctca gccagaataa gcgcgcgttg
    18181 cggatggtcc gtcgccagcg ccatttcata atagggtgca acggcaaaag cgcccggtaa
    18241 cggctgtttg ttatctaaca aatcgtgcat tcgcgggatc agcacccact gcaaccactc
    18301 cagtggttcc atggtgtcca taaagaacgg ttgggtacta ttaaattgat gcggctgggg
    18361 ttcatcgttt cgccagtgct gatgttcacg cagtaacgct tcaagcgcct gcaactggag
    18421 acgaacgcgg tcatgagtgg tcataaagca acctcaataa gaaaaactgc cgcgaaggat
    18481 agcaggaata aaaaaaggga gcactgtatt cacagcgctc ccggttcgtt tcgcagcatt
    18541 ccagctactt ttgttgctcc ctgctcatcc ttgacaactt ttcctctggc cttgcggcca
    18601 atcgttcatc ctgaactatt gcttcctgct cacaccaccc cgatgtgata cttcatcctg
    18661 aagtgtccct ggccttcctg acccaccgaa tcatcctgac cggttctcat tctccatcct
    18721 ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct tcatccagaa gcctttccct
    18781 gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc ctgatgttca cttcgttgtc
    18841 tgactccctg tcgacgaaga tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca
    18901 gacaatgcct taaggaaaat ttcatatgaa agttaagaat taacaatcaa cattttgatt
    18961 ttaaatgaaa ataacgattc ttatcttttt aagtattcgc ttatttcctg gcgatctcct
    19021 acagagtctg taagagatct ctcacaacca gatggggtat ttggattaca gaagaggctt
    19081 aagttgatta aggaattccg caagatttga ggctaaatgc gtccgtttac gtgttcccag
    19141 cgtctctttg cacacttcac cggagagatt acacaccgag atgacctcta actcttcttc
    19201 cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt ttctgagtca ccagatgacc
    19261 aatcagattt tcctgcaccc gacggaagtc gtcttcactc caggtttgca gcaatgtcag
    19321 tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg
    19381 aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc
    19441 ggtaaacggc tggggttgcc agtatacggc atcttcggta gttgaaataa tgcacggtga
    19501 aggaacaccg tacagttcct cacttaacgg ccagctttta tgctcttcat gccacgcatc
    19561 gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc
    19621 tcttcactta aaccagatac acttgtcgtt tagtttatct ggtttatgac ggtgaaacat
    19681 gtcttcttat gcaaaccatc aggcacttgc gggcctgact cttggaaaat caaccgatta
    19741 ccgggatacc tatgacgcca acctactgca aggcgttcca cgcagcctga atcgcgaccc
    19801 gctgggtctg aaagcggata acctgccttt tcacggtacg gatatctgga cgctgtatga
    19861 actttcctgg ctgaatgcga aaggtttgcc gcaggttgcc gtcggtcacg ttgaacttga
    19921 ttacaccagc gcaaatctga ttgagtcgaa gagttttaag ctctatctca acagttttaa
    19981 ccagacgcgt tttaataact gggatgaggt gcgccagacg ctggagcgcg acttaagcac
    20041 ttgcgcccag ggtaaggtta gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc
    20101 gataggtcat ttcaatggca cctgcattga tgaccaggat atcactatcg ataactatga
    20161 attcactact gactatctgg agaatgccac cagcggtgaa aaagtagtgg aagagacgct
    20221 tgtcagccac ctgctgaaat caaactgcct gatcacccat caaccagatt ggggttcgat
    20281 ccaaattcag tatcgtggac gccaaattga cagagaaaaa ctgctgcgtt acctggtctc
    20341 attccgtcat cacaacgagt tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt
    20401 acgcttctgc cagccagaga aattaagcgt ttacgcacgt tatacccgtc gtggcggtct
    20461 ggacattaac ccgtggcgca gtaatagcga ttttgtccca tcgaccacaa gactggttcg
    20521 gcaataaatt ttttctcaat tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat
    20581 caggcagggc tattgtaatc aaagggaatg acgatattcg tcccataagg agtttttctt
    20641 gattacacat attagcccgc ttggctccat ggatatgttg tcgcagctgg aagtggatat
    20701 gcttaaacgc accgccagca gcgacctcta tcaactgttt cgcaactgtt cacttgccgt
    20761 actgaactcc ggtagtttga ccgataacag caaagaattg ctgtctcgtt ttgaaaattt
    20821 cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg gaactgatta atcccccgga
    20881 agaggctttt gtcgatgggc gaattattcg cgctttgcag gccaacttgt tcgcggttct
    20941 gcgagacatt ctcttcgttt acgggcaaat ccataatacc gttcgttttc ccaacctgaa
    21001 tctcgacaac tccgtccaca tcactaacct ggtcttttcc atcttgcgta acgctcgcgc
    21061 gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg ggcggtcact caattaacga
    21121 aaacgagtat ttgtatgccc gtcgcgtcgg aaaccagctg ggcctgcgtg agctgaatat
    21181 ctgcaccggc tgtggtccgg gagcgatgga agcgccgatg aaaggtgctg cggtcggaca
    21241 cgcgcagcag cgttacaaag acagtcgttt tattggtatg accgagccgt cgattatcgc
    21301 cgctgaaccg cctaacccgc tggtcaacga attgatcatc atgccagata tcgaaaaacg
    21361 tctggaagcg tttgtccgta tcgctcacgg tatcattatc ttccctggcg gtgtgggtac
    21421 ggcagaagag ttgctgtatt tgctgggaat tttaatgaac ccggccaaca aagatcaggt
    21481 tttaccattg atcctcaccg gcccgaaaga gagcgccgac tacttccgcg tactggacga
    21541 gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat taccgcatca tcattgatga
    21601 cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg
    21661 cgacactggt gacgcttaca gctttaactg gtcaatgcgc attgcaccag atttgcaaat
    21721 gccgtttgag ccgtctcacg agaatatggc taatctgaag ctttacccgg atcaacctgt
    21781 tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa
    21841 agaagtcggt attcgcgcca ttgaagagtt tggtccttac aaaatcaacg gcgataaaga
    21901 gattatgcgt cgtatggacg acctgctaca gggttttgtt gcccagcatc gtatgaagtt
    21961 gccaggctca gcctacatcc cttgctacga aatctgcacg taatctcccc tcccccggtg
    22021 acgtttgttg ccgggttttc tcgtttttgg tcacttactc atcaactcat ttcatttgtt
    22081 atatgaatgt ttcttaccac cctcacgcga caaatatcat cacagttaat atgtcataca
    22141 atttatattg caacgcaaac gtttccctat tttcataaac cgtattttat cgctatgaaa
    22201 aag
//
LOCUS       DAZFDP010000071        22195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFDP010000071 DAZFDP010000000
VERSION     DAZFDP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1009)
                     /gene="malZ"
                     /locus_tag="ACHJ1G_002763"
     CDS             complement(<1..1009)
                     /gene="malZ"
                     /locus_tag="ACHJ1G_002763"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4764595.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RL"
     gene            complement(1165..2537)
                     /gene="proY"
                     /locus_tag="ACHJ1G_002764"
                     /pseudo
     CDS             complement(1165..2537)
                     /gene="proY"
                     /locus_tag="ACHJ1G_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
     gene            complement(2613..3932)
                     /gene="brnQ"
                     /locus_tag="ACHJ1G_002765"
     CDS             complement(2613..3932)
                     /gene="brnQ"
                     /locus_tag="ACHJ1G_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4764596.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4339..5634)
                     /gene="phoR"
                     /locus_tag="ACHJ1G_002766"
     CDS             complement(4339..5634)
                     /gene="phoR"
                     /locus_tag="ACHJ1G_002766"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4764597.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5692..6381)
                     /gene="phoB"
                     /locus_tag="ACHJ1G_002767"
     CDS             complement(5692..6381)
                     /gene="phoB"
                     /locus_tag="ACHJ1G_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4764598.1"
                     /translation="MTRRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6571..7773
                     /gene="sbcD"
                     /locus_tag="ACHJ1G_002768"
     CDS             6571..7773
                     /gene="sbcD"
                     /locus_tag="ACHJ1G_002768"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4764599.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7770..10913
                     /gene="sbcC"
                     /locus_tag="ACHJ1G_002769"
     CDS             7770..10913
                     /gene="sbcC"
                     /locus_tag="ACHJ1G_002769"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4764600.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11039..12223
                     /gene="araJ"
                     /locus_tag="ACHJ1G_002770"
     CDS             11039..12223
                     /gene="araJ"
                     /locus_tag="ACHJ1G_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4764601.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12366..13274)
                     /gene="mak"
                     /locus_tag="ACHJ1G_002771"
     CDS             complement(12366..13274)
                     /gene="mak"
                     /locus_tag="ACHJ1G_002771"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4764602.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13399..14310
                     /gene="rdgC"
                     /locus_tag="ACHJ1G_002772"
     CDS             13399..14310
                     /gene="rdgC"
                     /locus_tag="ACHJ1G_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4764603.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14388..14471)
                     /locus_tag="ACHJ1G_002773"
     CDS             complement(14388..14471)
                     /locus_tag="ACHJ1G_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764604.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(14468..14809)
                     /locus_tag="ACHJ1G_002774"
                     /pseudo
     CDS             complement(14468..14809)
                     /locus_tag="ACHJ1G_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14957..15241)
                     /gene="ppnP"
                     /locus_tag="ACHJ1G_002775"
     CDS             complement(14957..15241)
                     /gene="ppnP"
                     /locus_tag="ACHJ1G_002775"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4764605.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15313..15990)
                     /gene="aroM"
                     /locus_tag="ACHJ1G_002776"
     CDS             complement(15313..15990)
                     /gene="aroM"
                     /locus_tag="ACHJ1G_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4764606.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16248..16439)
                     /gene="yaiA"
                     /locus_tag="ACHJ1G_002777"
     CDS             complement(16248..16439)
                     /gene="yaiA"
                     /locus_tag="ACHJ1G_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4764607.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16489..17013)
                     /gene="aroL"
                     /locus_tag="ACHJ1G_002778"
     CDS             complement(16489..17013)
                     /gene="aroL"
                     /locus_tag="ACHJ1G_002778"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4764608.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17196..17654)
                     /gene="yaiI"
                     /locus_tag="ACHJ1G_002779"
     CDS             complement(17196..17654)
                     /gene="yaiI"
                     /locus_tag="ACHJ1G_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4764609.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17774..18583
                     /gene="proC"
                     /locus_tag="ACHJ1G_002780"
     CDS             17774..18583
                     /gene="proC"
                     /locus_tag="ACHJ1G_002780"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4764610.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18600..19715)
                     /gene="adrA"
                     /locus_tag="ACHJ1G_002781"
                     /gene_synonym="dgcC"
     CDS             complement(18600..19715)
                     /gene="adrA"
                     /locus_tag="ACHJ1G_002781"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4764611.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19817..20137)
                     /gene="psiF"
                     /locus_tag="ACHJ1G_002782"
     CDS             complement(19817..20137)
                     /gene="psiF"
                     /locus_tag="ACHJ1G_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4764612.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20256..21671)
                     /gene="phoA"
                     /locus_tag="ACHJ1G_002783"
     CDS             complement(20256..21671)
                     /gene="phoA"
                     /locus_tag="ACHJ1G_002783"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO4764613.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21772..22032)
                     /gene="iraP"
                     /locus_tag="ACHJ1G_002784"
     CDS             complement(21772..22032)
                     /gene="iraP"
                     /locus_tag="ACHJ1G_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4764614.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         5569 a         5948 c         5537 g         5141 t
ORIGIN      
        1 ccagccgcca gccgtccata ctccacggcg ctttcagcca atggcggaca atactgtctt
       61 ccccgcgata aatttcattc accagacttt ccgactgata atccagcttc ggcaagctgg
      121 catagccaag ccagtcgagc gccgtgccat catcactaaa cgagtaccag tcgcgccagg
      181 gcgattcagg gttgtgacaa gctccccccg tgccacgatt gtgcctgtca aaccaggcat
      241 gggaatcgcc actgtggtta aacacgccat ccagcaccag ccgcattccc agctgctgcg
      301 tattgtggcg taagcgcagc aacgccccat caccgtcaaa ctgcggatcg acatggcgat
      361 aatcctcggt atcgtattta tgtacgctgg gagctttaaa caccggattg agatacagcg
      421 ccgtcacgcc aagctttttc agatacggca gtttttcgct tatcccgtcc agatcgccgc
      481 catagaacgt tgatccgccc gcctgcgccg tgaccggttc atcccagtca cgcaagatga
      541 tctcttgtcc ggctgcatga tggtaataga catgatcctg ttcagcttca cgaggaagac
      601 tacgcgcaaa acgatcaggg aagatctgat aaaaaatctg atccgcagcc cattgtgggc
      661 cgatatccgg tacatcgacg gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc
      721 cctgcggtgt aaaccaacgc tggcgatcgt gccacagcag tttgaaactg taacgccgcc
      781 ggggttgtcc gctggagaga tcaatcgccg cacgccatgc ggtgacgcca ggctgctgct
      841 gactgcgctg cttatgcatc gatactgaca tttcttcgtt atcgtgttct gtacgcagca
      901 taatgcgctg cggtgggtct tcgcccgtca gccacagtgt aatgagcagt tgatctttgc
      961 tttgtttaac aaatgggggc accggcaggt gccatgcatt taacatcata attcccctgt
     1021 gatgaaaatc tgatcacctt gccatagcct aatgcaggat cactcatcat ctgaaggata
     1081 tttgggggag gaggacgggg gcggataact ttcggaatag ccggatcagg atttcctcct
     1141 gaaccggctt acagaacgca agtattattg gttttcagcc agctgacgat cgtggcggcg
     1201 tttaaacatc cagccaatca acagcacaac aatccacgcg aaaccgacat acagcgagat
     1261 acgcgtatcc gggtgataac caatcaaccc gataataaag agcaggaaaa tcagccggcc
     1321 gatggtcgtt gctaccccac ccggcacttt aaatttcagc gccttaactt cttctggcgg
     1381 caaacggcga cggaaggcaa tttgcgacag caggatcata atccacaccc acaccgtggc
     1441 gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc ggcatgatgt agttcagata
     1501 caccgcaaac agcagcgcgg tagtcatcac cagcaccgta acccacggaa taccgcgacg
     1561 cgacgttttg ctgaaaattt tcggcgcgct gccctgctct gccataccgt ggagcatacg
     1621 gcctacgcca aatacgtcac tgttaattgc cgacagcgaa gcagtcagca caacaaagtt
     1681 aagaatgctg gcggcaaagg taatgcccat atgctggaac gtcagcacga acgggctacc
     1741 ggcagtgcca acctgattcc acgggtagat agacataatg acgaacagcg taccgacgta
     1801 gaacaccaga atacgcatcg gcacggagtt aatcgcacgc ggtatcgatt tctcaggatc
     1861 tttcgcttca ccggcggtaa tcccgataat ttcgatccca ccgtaagcaa acatcaccat
     1921 ttgcaacgac atcaccatcc caagccagcc gttactgaag aagccgccgt tgctccacag
     1981 gttatgaata ccggtcggtt gcccgccgtt gccaatcccc cagatgatga tgccgaaacc
     2041 ggcgacaatc atgatgatga tggtggcgac tttaaagaac gagaaccaga attccagctc
     2101 accgaagacc ttcacgctca tcaggtttac ggcgcaaatg atcagcacca cgctcagtac
     2161 ccaaatccag tgcggcaccg tcgggaacca gacacccata tagataccaa aagcggtcac
     2221 atcggcgatg gcgacaataa ggatttcaaa gcaataggtc cagccggtaa tgtaacctgc
     2281 cagcgggccg aggttttcct gcgcataacg cgagaaagag ctggctgccg ggttatgtac
     2341 cgacatttcc cccagcgcac gcataatgat atacgccgcg ataccaccga taatataggc
     2401 caacaacacg ctcggaccgg ccattttgat ggcgtctgcc gaaccgtaaa acagcccggt
     2461 gccaattgct gaacccagtg ccataaagcg tatgtggcgg tacttagccc acgctttagc
     2521 ttgttcttac tttccatcaa ttcctaaccc atcaacacaa taaaaaacca cggggcatcg
     2581 cagccccgtg gttaaaacaa atgttcagtg atttagtgag cgctggaggt cacctgacga
     2641 cctgccgcgc gatcccagat aatggccaga accaccatca ccactgttgg cattaaccac
     2701 gccagacctt gttcggccag cggtaaacgc tgcgcccagg acggtaagat atcgctgaat
     2761 gcagatgctt tgatcccgtc gagaatacca aaaagcaggc tgataaacat cggcggagca
     2821 atcacgcggg acgaattatg ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata
     2881 cacggcggat aaatagcggt cagcaccggt acggagatct ggatcagctg gcttaagccg
     2941 aggttagaaa ccaccatcga gaagccgccg aggataaaca ccagcgtacg ataagagagc
     3001 ggtacgtact gggcaaagaa ttctgcacaa gcacaggtca ggccaactgc cgttaccagg
     3061 caggcgatga agattaacgc cgccagcagg aagctaccgc cgccgccaaa ggtgtgctga
     3121 acgtaagcat gcagaatagc agcgccgttt gcagactgat cgaccagcga cgcgctgtct
     3181 gaacccagac ggaacagcgc caggtacagc agagtcagac caacacccgc catcaggcca
     3241 gcccagacgg tataacgggt cagcagacgc gcttcggtaa cgccacgaga acgcgccgcg
     3301 ttaacaataa cgataccaaa caccattgcg cccagcgtat ccatggtcag atagccgtta
     3361 acgaagccgt tagaaaacgc agcgttttga taagcctcag tcgccgtgct gatagaaccc
     3421 gccggccaga caatcgcggc aacagacagg atgaccagcg cgataatttt cagcggcgca
     3481 aggaagttgc ccacggtatc cagcagcttg cccggataga gcgaaaccag aataacgata
     3541 gcgaaataga ccaggctgta gataaacagc ggcagcgcgg aatcacccgt cagcggcgca
     3601 atccccactt caaaggaaac ggtagctgta cgcggcgtag cgaaaagcgg ccccaccgcc
     3661 aggtaacaaa ccgttgccag cagtacgcca gcgactttac cgattggcgt gctgaggctg
     3721 tcaacaccgc cgccaacttt tgccagcgcc actaccgtta ataccggcag gccaacggca
     3781 gtaatgagga agccgaatgc cgcagtccag acgtgttcgc ctgcctgtaa gccgaccatt
     3841 ggagggaaaa taatgttacc tgcgccgacg aacaacgcaa atgtcataaa gcccagagcg
     3901 atgatatcgc gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg atgtggtgtt
     3961 gtaacgttaa atttttgtgc caatccctgg ggatgataca gcggtatatt taaacaattt
     4021 tagcgggaaa tactcccaat caggcgcggc gaagaatagt ttttcgattt accgcgttaa
     4081 tgcagtcagt atgacagcac ctgaagcgca atttaaacgc ttataacgtt ttaaagcaag
     4141 gagggatcta aaaaccagag gtatatagcg ataagaaatc accaaccagt ataaaacact
     4201 atttccgtga aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc gttgtaaaat
     4261 ctaacgcccg tattttgact gcaaagaaaa taagccagca tcggctggct tatggcaata
     4321 aaagatgaca aaggcggatt aatcgctgtt tttggcaatt aaacgttccg ggataacaaa
     4381 actgaaacgt gttccttttc ctactgtact ctcaatattc aggcgacttt cgtgatgatt
     4441 caccgcatgt ttcacgatcg ctaaccctaa tccgctaccg ccggtttgcc gggaacgcgc
     4501 tttatcaacg cgataaaaac gctcggtcag gcgcggaata tgctccggtg caatgcccgg
     4561 tccgttatct tcaacgctaa attcggcacc gtgcggcact cgcagccagc gtacggtgat
     4621 atgcgtgcct tccggcgtat gattcacggc gttatagacc aggttcgaaa tcgcactgcg
     4681 tagctgatct tcgttgccag acaccttgag gccgttatct atctcaaagg taaatgtctg
     4741 ttttttctga ctcagagtct gagcctcgcg ctcaacaacg cgcagcatca tcggcacatc
     4801 aaccttttca ttgagcaatt gcgtcggcgc ggcttcaatt ttcgacagcg ttagcaactg
     4861 cttcaccagc ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg ctttttcgcg
     4921 taccgcgcct tccagcggct gctcatccat catctccagg taaccctgta acacggtcaa
     4981 tggcgtacgt aactcatggc tcacgttggc aaaaaagtta cgccgcgccc cttccagttg
     5041 atgcatttgc gtgacatcac gcgccaccat cagcaactgt ttgtgggtat aaggcatgac
     5101 gcgaatttcc agatgccgcc cggtgttgag caccagattg agcgggcgag aaaaatcacg
     5161 cgttttcaga tattgcgtaa actccgggta acgcagcagg ttaaggatgt tctgcccgtt
     5221 atcttccggc cagcgcaaac caagaatttg ttgcgccaga ccgttacacc agaaaatacc
     5281 gccctcttcc gtggtcagca ccactgcgtc gggcagcgac tccgcgccgc tacgaaagcg
     5341 tttaatcaga ttgcccagtt cacggcggcg ttttttattt cgcagctgca tctggtgtaa
     5401 gccgtatagt agcggttccc agctaccacg ccccggtggc ggggtcatac tacgatccac
     5461 ccacagccac catgaaaggc gcaataaatt ccagaaatgc cagataagca gtcccgttac
     5521 cgatgccagc aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga aagccgggag
     5581 gcagcaaagt agcagctcca gcaccagcct tttccacgac agccgttcca gcacgtaaga
     5641 tactccagtt aagaaatcat aagccctgct ctgcgtccga tgagcaaggc gttaaaagcg
     5701 ggttgaaaaa cgatatcctg taccgcgcac ggtctgcacc atgcggtcat gcccgccggg
     5761 ctccagtgct ttacgcaggc gacgaatgtg gacatcgacc gtgcggtctt ccacatacac
     5821 gttagttccc cagacgtggt ttaacagctg ctcgcggctg tacacgcgct caggatgcgt
     5881 cataaaaaag tgcagcagtt taaattctgt cggccccatc tccagcggct cttcgcccgc
     5941 catcactcgg tgagatgtcg ggtcgagact taatccctgc atctcaatca cctcttccac
     6001 cgccattggc gaaatacggc gcattaccgc tttgattcgc gccaccagct ccttcggcga
     6061 aaacggcttg gtgatatagt catccgcgcc ggtttcaagg ccgcgcacgc gatcttcttc
     6121 ttcccctctg gcggtcaaca tcaccactgg aatatcccgg gtcatcgact cgcgcttgag
     6181 gtgtttgatg aactggatac cggagccgcc aggtaacatc cagtcaagga gaattaaatc
     6241 cggccagggt tcattcagtt gattcacagc actgtcataa tcttccgctt cgaccggctg
     6301 aaagccattt tgttcgagca cgaagcagac catttcgcga attggagctt catcttctac
     6361 gaccagaata cgtctcgtca tgatttgccc tgttgtaata aataggttgc gatcattaat
     6421 gcgacgtcat tatgcgtcag atttatgaca gatttatgaa aagctcgtcg cacatatctt
     6481 caggttattg atttccgtgg cgcagaaaaa agcaaatggc acatctgttt gggtataatc
     6541 gcgcccatgc tttttcgcca gggaaccgtt atgcgcatcc ttcacacctc agactggcat
     6601 ctcggccaga acttctacag taaaagccgc gaagctgaac atcaggcttt tcttgactgg
     6661 ctgctggaga cagcacaaac ccatcaggtg gatgcgatta ttgttgccgg tgatgttttc
     6721 gataccggct cgccgcccag ttacgcccgc acgttataca accgttttgt tgtcaattta
     6781 cagcaaactg gctgtcatct ggtggtactg gcaggaaacc atgactcggt cgccacgctg
     6841 aatgaatcgc gcgatatcat ggcgttcctc aatactaccg tggtcgccag cgccggacat
     6901 gcgccgcaaa tcttgcctcg tcgcgacggg acgccaggcg cagtgctgtg ccccattccg
     6961 tttttacgtc cacgtgacat tattaccagc caggcggggc ttaacggtat tgaaaaacag
     7021 cagcatttac tggcagcgat taccgattat taccaacaac actatgccga tgcctgcaaa
     7081 ctgcgcggcg atcagcctct gcccatcatc gccacgggac atttaacgac cgtgggggcc
     7141 agtaaaagtg acgccgtgcg tgacatttat attggcacgc tggacgcgtt tccggcacaa
     7201 aactttccac cagccgacta catcgcgctc gggcatattc accgcgcaca gattattggc
     7261 ggcatggaac atgttcgcta ttgcggctcc cccattccac tgagttttga tgaatgcggt
     7321 aagagtaaat atgtccatct ggtgacattt tcaaacggca aattagagag cgtggaaaac
     7381 ctgaacgtac cggtaacgca acccatggca gtgctgaaag gcgatctggc gtcgattacc
     7441 gcacagctgg aacagtggcg cgatgtatcg caggagccac ctgtctggct ggatatcgaa
     7501 atcactactg atgagtatct gcatgatatt cagcgcaaaa tccaggcatt aaccgaatca
     7561 ttgcctgtcg aagtattgct ggtacgtcgg agtcgtgaac agcgcgagcg tgtgttagcc
     7621 agccaacagc gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa tcgccgtctg
     7681 gcactggaag aactggatga atcgcagcag caacgtctgc agcatctttt caccacgacg
     7741 ttgcataccc tcgccggaga acacgaagca tgaaaattct cagcctgcgc ctgaaaaacc
     7801 tgaactcatt aaaaggcgaa tggaagattg atttcacccg cgagccgttc gccagcaacg
     7861 ggctgtttgc tattaccggc ccaacaggtg cggggaaaac caccctgctg gacgccattt
     7921 gtctggcgct gtatcatgaa actccgcgtc tctctaacgt ttcacaatcg caaaatgatc
     7981 tcatgacccg cgataccgcc gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag
     8041 cgtaccgtgc attctggagc cagaatcggg cgcgtaacca acccgacggt aatttgcagg
     8101 tgccacgcgt agagctggcg cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag
     8161 ataagctgga actgacagcg acgttaaccg ggctggatta cgggcgcttc acccgttcga
     8221 tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg
     8281 aattgctcga ggagttaacc ggcactgaaa tctacgggca aatctcggcg atggtttttg
     8341 agcagcacaa atcggcccgc acagagctgg agaagctgca agcgcaggcc agcggcgtcg
     8401 cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta cttactgacg
     8461 aagaaaaaca gttacttacc gcgcagcagc aagaacaaca atcgctaaac tggttaacgc
     8521 gtcaggacga attgcagcaa gaagccagcc gccgtcagca ggccttgcaa caggtgttag
     8581 ccgaagaaga aaaagcgcaa cctcaactgg cggcgcttag tctggcacaa ccggcacgaa
     8641 atcttcgtcc acactgggaa cgcatcgcag aacacagcgc ggcgctggcg catattcgcc
     8701 agcggattga agaagtaaat actcgcttac agagcacaat ggcgcttcgc gcgagcattc
     8761 gccaccacgc ggcgaagcag tcagcagaat tacagcagca gcaacaaagc ctgaatacct
     8821 ggttacagga acacgaccgc ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc
     8881 agttctccca acaaaccagc gatcgcgagc atctgcggca atggcagcaa cagttaaccc
     8941 atgctgagca aaaacttaat gcgcttgcgg cgatcacgtt gacgttaacc gccgatgaag
     9001 ttgctaccgc cctggcgcaa catgctgagc aacgcccact gcgtcagcgc ctggtcgcgc
     9061 tgcatgggca gattgttccc caacaaaaac gtctggcgca gttaatggtc actatccaga
     9121 atgtcactct ggaacagacg caacgtaatg tcgcacttaa cgaaatgcgc cagcgttata
     9181 aagaaaagac gcagcaactt gccgatgtga aaaccatttg cgagcaggaa gcgcgcatca
     9241 aaacgctgga agcccagcgt gcacagttac aggcgggtca gccttgtcca ctttgtggtt
     9301 ccaccagcca tccggcggtc gaggcgtatc aggcgctgga gcctggcgtt aatcagtctc
     9361 gattactggc gctggaaaac gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg
     9421 ggcaactgga tgcattaacg aagcagcttc agcgcgatga aaacgaagcg caaagcctcc
     9481 gacaagatga gcaagcactt actcaacaat ggcaagccgt cacggccagc ctcaatatca
     9541 ccttgcagcc acaggacgat attcaaccgt ggcttgatgc acaagatgag cacgaacgcc
     9601 agctgcggtt actcagccaa cgacatgaat tacaagggca gattgccgcg cataatcagc
     9661 aaattatcca gtatcaacag caaattgaac aacgccagca gcagctctta acggcattgg
     9721 cgggttatgc actgacattg ccacaggaag atgaagaaga gagctggttg gcgacacgtc
     9781 agcaagaagc gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg caaaaccgta
     9841 ttcagcagct gacgccgatt ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg
     9901 aagaaactgt ggcgctggat aactggcggc aggttcatga acaatgtctc gcattacaca
     9961 gccagcagca gacattacag caacaggatg ttctggcggc gcaaagtctg caaaaagccc
    10021 aggcgcagtt tgacaccgcg ctacaggcca gcgtctttga cgatcagcag gcgttccttg
    10081 cggcgctaat ggatgaacaa acactaacgc agctggaaca gctcaagcag aatctggaaa
    10141 accagcgccg tcaggcgcaa acgctggtca ctcagacagc agaaacgctg gcacagcatc
    10201 aacaacaccg acctgacggg ttggctctca ctgtgacggt ggagcagatt cagcaagagt
    10261 tagcgcaaac tcaccaaaag ttgcgtgaaa acacgacgag tcaaggcgag attcgccagc
    10321 agctgaagca ggatgcagat aaccgtcagc aacaacaaac cttaatgcag caaattgctc
    10381 aaatgacgca gcaggttgag gactggggat atctgaattc gctaataggt tccaaagagg
    10441 gcgataaatt ccgcaagttt gcccaggggc tgacgctgga taatttagtc catctcgcta
    10501 atcagcaact tacccggctg cacgggcgct atctgttaca gcgcaaagcc agcgagacgc
    10561 tggaagtcga ggttgttgat acctggcagg cagatgcggt acgcgatacc cgtacccttt
    10621 ccggcggcga aagtttcctc gttagtctgg cgctggcgct ggcgctttcg gatctggtca
    10681 gccataaaac acgtattgac tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg
    10741 aaacgctgga taccgccctt gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg
    10801 gtgtgattag ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa
    10861 agatcaacgg cctaggctac agcaaactgg aaagtacgtt tgcagtgaaa taactattca
    10921 gcaggataat gaatacagag gggcgaatta tctcttggcc ttgctggtcg ttatcctgca
    10981 agctatcact ttattggcta cggtgattgg tagccgttct ggtagttgtg atggtggtat
    11041 gaaaaaagtc attttatctt tggctctggg cacgtttggt ttggggatgg ccgaatttgg
    11101 cattatgggc gtgctcacgg agctggcgca taacgtagga atttcgattc ctgctgccgg
    11161 gcatatgatc tcgtattatg cgctgggagt ggtggtcggt gcgccaatca tcgcactctt
    11221 ttccagccgc tactcactca aacatatctt gttgtttctg gtggcgttgt gcgtcattgg
    11281 caacgccatg ttcacgctct cttcgtctta cctgatgctt gccattggtc gcctggtttc
    11341 cggctttccg catggcgcat tttttggcgt cggcgcgatc gtgttatcaa aaattatcaa
    11401 acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc gggatgacag tcgccaattt
    11461 gctgggcatt ccgctgggaa cgtatttaag tcaggaattt agctggcgtt acaccttttt
    11521 attgatcgct gtttttaata ttgcggtgat ggcatcggtc tatttttggg tgccggatat
    11581 tcgcgacgag gcgaaaggaa agctgcgcga acaatttcac tttttacgca gcccggcccc
    11641 gtggttaatt ttcgccgcca cgatgtttgg caacgcaggt gtgtttgcct ggttcagcta
    11701 cgtaaagcca tacatgatgt ttatttccgg tttttcggaa acggcgatga cctttattat
    11761 gatgttagtt gggctaggga tggtgttggg aaatatgcta agtggcagga tttcaggacg
    11821 ttattcacca ctgcgcattg cagcagtgac tgactttata attgtactgg cactgctgat
    11881 gctctttttc tgctgcggca tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc
    11941 gggattattt gccctttcag cacctctgca aatattgtta ctacaaaacg ccaaaggcgg
    12001 agagttatta ggtgccgcag gtgggcaaat agcgtttaac ctcggtagcg ccgtcggcgc
    12061 atattgcgga ggtatgatgc tgacgctggg gctggcatat aattacgtgg cgctgcctgc
    12121 cgccctgctt tcgtttgctg cgatgtcatc gttgctgctg tatggtcgct ataagcgcca
    12181 gcaagcggcg gatagtccgg tgctggcgaa accactgggg tagattactg tcacggtgcg
    12241 cgttgcttat tgccggatgc ggcgtgaacg ccttatccgc cctacgcggt tctggcacat
    12301 tttgtaggcc tgataagacg cggcaagcgt cgcatcaggc atgatgcgcc aattgcctac
    12361 gttttttact cttgtggcca taaccacgca gcgccgcgta cgccgctgga atcaccgtgc
    12421 ttcgccttac gcaccggcgt ttcacattcg ccgccgaaga caaattgttt aatcaactgc
    12481 ggaaccgttt gatataaacg gtctacattg ctcatcccgc cccccaggac aatcacatcc
    12541 ggatcgagaa tattcacgac atgtgccagc gattttgcca gccgcagctc gtagcgacgc
    12601 aatgccagtt ccgctaccgg atcgctttct tcaaccaggc tgataatttc actgcctttc
    12661 agcgcatgtc cgctcaaacg acgataatcc gtcgcgaatc cagtgcccga aataaaggtt
    12721 tcaatacaac cttgtttacc gcaataacaa gggacttcct cgcgataacg cagttcgtct
    12781 tcgtccatcc acggtagcgg attgtgtccc cactcacctg ccgtgccatt gccgccgata
    12841 tgcgcccgcc cattgaatgc cacgcccgcg ccgcatcccg tgccgataat cacggcaaat
    12901 accgtctgcg ctcccgctgc cgcgccatct accgcttctg aaaccgccag acagttggcg
    12961 tcatttgcca gccgcacttc ccgctgcaac cgcgcgctta agtctttatc gaatggctga
    13021 ccgttgagcc aggttgaatt ggcattcttc accacaccgg tgtagggcga aatcgaacct
    13081 ggaatgccca tacctaccgt tccgcgctgc cctgtcgcct gctccgccat atcaaccaac
    13141 gtggcgatcg tttcaatagt ttgccggtaa tcatcacgcg gcgtgggcag acgatggcgg
    13201 tacaactgct cccctgcatc gcccagtgca atcacttcag ttttggtgcc gcctaaatcg
    13261 atacctatac gcacggtact ctccttattt ttttcaatat caatagcgta gagacggaca
    13321 accggattgg caatgcaagg ccgccgacaa ttcgttatca tgcccgctaa atttaacgac
    13381 aaggccgtgg aaattatcat gctgtggttc aaaaatttaa tggtttaccg tcttagccgc
    13441 gagatttcgc tgcgtgcaga agagatggaa aaacagctag cctcgatggc atttacccca
    13501 tgcggcagcc aggacatggc gaagatgggc tgggttcctc cgatgggatc gcacagcgat
    13561 gcgttaacgc acgttgccaa tggtcaaatt gttatctgcg cgcgcaaaga agaaaaaatc
    13621 ctcccgtctc cggtgattaa acaggcgctg gaagcgaaaa tcgccaaact ggaagcggaa
    13681 caggcgcgta agctgaagaa aaccgaaaaa gattcgctga aagacgaagt gctgcactct
    13741 ctgctgccgc gtgctttcag ccgttttagc cagacaatga tgtggatcga cacggttaac
    13801 ggtttgatta tggtggactg cgccagtgcc aaaaaagcgg aagatacgct ggcattactg
    13861 cgtaaaagcc tggggtcgtt accggttgta ccattgagca tggaaaaccc gattgaactg
    13921 acgctgaccg aatgggttcg ctccggtagt gcggcacagg gcttccagct gcttgatgaa
    13981 gccgagctga aatcgttgct ggaagatggc ggcgtgatcc gcgcgaagaa acaagatctg
    14041 accagcgaag agatcaccaa tcacattgaa gccggaaaag tggtgactaa actggcgctc
    14101 gactggcagc agcgcattca gtttgtgatg tgcgacgatg gttcgctcaa gcgtctgaag
    14161 ttctgcgacg agctgcgcga tcaaaacgaa gatatcgacc gtgaagattt cgcccagcgt
    14221 tttgatgccg atttcatcct gatgactggt gaactggcag cgttaattca aaacctgatt
    14281 gaaggattag gtggcgaagc acaacgttaa ttgctgattt tcctttaatg ccggatgcga
    14341 cgcctgccgc gtcttatccg gcgtacgaag ccacaccagg catataatta ttcgctacgg
    14401 cgggcaataa tttttagcgc agcaatatta tgcgttttat gctgtaactt gctccatgga
    14461 cgttgtgtca ttgtttttcc tcaagccagt gacgcgcttc actgcgaatt ttccgcattg
    14521 ccccggtttt cactaaatcc cgtaacagcg aaagatcggg ttgttcgaca aataagagta
    14581 atgatggatc ctcttttaac cacgtcgttt tcacatcggc agcgagctgt ggattattca
    14641 tagctagcga ccgatgccga gactcggtca atgtcgttaa caacgacgcc ggggtatgtg
    14701 cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc
    14761 attcatcatc tgatttatct tttatcgtag ccagttccgc aacaatatct ggagatcttc
    14821 actgatattt atatcaacct tgatgaatta tgaatatacc ggaaattgaa aaaatcgcag
    14881 caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc ctcccgttca
    14941 aggggaaggc gaggaattac agatagcggc acagataaga ggtgggttcg gcaacttgca
    15001 gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac acctgccagt
    15061 cggtcgcgtc aggcagtaac acattcagcg cgccactgat tactgtcatc tcttccggct
    15121 cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg cgaccagtgc
    15181 tgctgctgga aaagccgatt gatttcactt tgccggaaaa gtactcatta ctttgaagca
    15241 taaactggcc ctgtctggta gttgaacatc tacttcacta taggggccag aggcgctggc
    15301 tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata cgttagacag
    15361 caagacagga acatcgagct gtttttgcag taaatcgcga tgacgttggt gaaatcctaa
    15421 acaatccagc atgatgacat cagccccttt tgccagtaat tctttcccgg catcaatgat
    15481 tttttgttct gaatcatgaa tggggttgcc caatgaaaat accggcgatt tctgcaaaat
    15541 ttgccatttt tgcgcctgaa cggtcagcat ctcctcaacc ggaacgataa cccccacctg
    15601 atgatcttca acaatagagg aaaccagtgg aggcaatatt cgcgacggct caagaaagat
    15661 cgtattacgc gcagtcatac tactaatgtt tgctgtactc attaataaaa tgacgtcata
    15721 accctgatta tcgagcactt caaccacacc ttgcaggtct cgctccactt tacgacgcga
    15781 aacatgggcc agctggttgt catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg
    15841 cgcgtactcc gccatcactt cttcacgact taacttcccc agcaagctat gatgggaaat
    15901 attatcttcg tcaatgtatt ccgtcaggag cggcaaaacc tcctgcatag gtacaatgcc
    15961 gatggtcagg atcgccaacg acgcactcat tgttcatcca ccttactttt ctttcgtcgt
    16021 taattaccgg gcaagtgtga agcaccactg ctgacactta cttgtgctac aaatgacaaa
    16081 aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc cagtaatgga
    16141 ttgttattgt ttaatattct aattattaga aaaacatgaa ttatgaaaaa tgtgacgcag
    16201 atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcggtca ctctttgtca
    16261 gggtcctcat agcgtttttt cgcatccatc gcttcctgag ccgtcggatg ttcactgatc
    16321 aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggc
    16381 tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc
    16441 gttcttcctt atttcacggg atgaacgtta agtataggcg ctctaaaatc aacaattgat
    16501 cgtctgtgcc agggcgctac gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc
    16561 gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac
    16621 ttcttcgctc agcggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg
    16681 cagtcggtta accaggactg atactggcgc acacaaataa accacgatcc cgttattttg
    16741 catgaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga
    16801 tggcgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg cccactcttc
    16861 cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact gatcggtatc
    16921 gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc
    16981 ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat
    17041 gcggcgaaag ccggtgtcat gagaatagcg atcatgccat caaactagta caatttcgat
    17101 tgtaaagaaa aaattccact taaagtgaaa atctcaatac atcccttact ataccaataa
    17161 atattcaaga atgaagtgta aataataaat tacatttagc cacgactacg ttgcacttcc
    17221 agccaccact tctccagctc cgcggcaaag gcctggcggt cacgttgtga aaggctatct
    17281 ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg
    17341 cgctcacgaa tggtcgctgg cgtgtaacgt tcgccgcgcg gattaagcgc cgctgcgcct
    17401 ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca
    17461 cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga
    17521 ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc
    17581 atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc
    17641 acccaaattg tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc
    17701 cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg
    17761 caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgcggcaata tgggaaaagc
    17821 cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatacac
    17881 cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc
    17941 ggcgcaagaa gtggcgcaaa tcgccgacat catttttgct gccgttaaac ctggcatcat
    18001 gattaaagtg cttagcgaaa tcacctccag cctgaataaa gactctctgg tcgtttctat
    18061 tgctgcaggt gtcacgctcg accagcttgc ccgcgcgctg ggccatgacc ggaaaattat
    18121 ccgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa
    18181 cgcgctggta accccagaag ataccgctga tgtgctgaat attttccgct gctttggcga
    18241 agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc
    18301 agcctacgta tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc
    18361 acgcgcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct
    18421 ggaaacggga gaacatccgg gggcactgaa agatatggtc tgctcaccgg gaggcaccac
    18481 cattgaagcg gtacgcgtac tggaagagaa aggcttccgt gccgcagtga tcgaagcgat
    18541 gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgacttt cgccggacgt
    18601 caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtaaag cgccaaatct
    18661 gccgatttca accactcacg atagtgactc atttgtgggt tcagcggcgc aacccccaca
    18721 ctaatccgta aagttacctg tggcgtattc ggcaagcgta acgtgtttag cccttcacgc
    18781 acccgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat tactgcaaac
    18841 tcatcgccgc caaaccgacc aatcacatcg ctaccgcgca gggtaatttg taactgtcgg
    18901 gtaagcgcca caatcgcttc atcgcccaca tcatgacccc aggtatcgtt gatgctcttg
    18961 aaatggtcga tatcgataat cagtaacgtt gcatcgcgat tatgccgccg acagttatca
    19021 aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt catgccgtcg
    19081 cgggtactca tgacctgcaa cctgcgttta tgttccgcca gtttggttgc cgtctggtag
    19141 ctgacccagc caaacagcag aggataaacg acaataatgg gaagggagag ccaccattcc
    19201 agcggcgcac tattgaacga cacggtaatg cccgtaagct cgagggtgac aaggcaggaa
    19261 accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc
    19321 agacacataa tcatcaacat agcggtggaa ggcagcacgt ttacgcccat tacgcctacc
    19381 cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg
    19441 acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac
    19501 accagccacc accagcccgg cggcgggtgc gaaaccagcg ttgaagcaat cggtaagaac
    19561 atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg cagcccggac
    19621 cgctgatgtt cgttttgagg agttaaagga ggttcctccc catgcgccgc cgcttttttg
    19681 aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc ccaaattata
    19741 gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccactttcgt agctcacccg
    19801 ttagcactat caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat
    19861 catcaccttt cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct
    19921 gtggcgtcaa acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt
    19981 aggtcttacg agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggatgtca
    20041 tacgctgttg ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca
    20101 gaccaaaaag caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt
    20161 aaccatgagc gtatgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac
    20221 cacccggcag cgaaaattca ctgccgggcg cggttttatt tcagccccag ggcggctttc
    20281 atggtgtaga agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca
    20341 tacgccgcaa tacgcaactg actgccggta tgttcttgtg aatcctcttc ggagttcccg
    20401 tagctcatca ccatcactgc gccatctttg gtatttagcg cctgggtgag gcccggagct
    20461 ttggtatccg gcgcgacaat ctggctggcg tgggcgtgat cagcggtgac tatgaccagc
    20521 gtgttgccat cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc
    20581 gtctcgccaa tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct
    20641 tcaacttgca ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc
    20701 atctgcgcca gggttggtac actgtcatta cgttgcggat taggcgtaca ggtgactgcg
    20761 ggcttatcga tattgccgtg gtacgttgct ttcggtccta gccagcgcac tggcatattg
    20821 ccgtcagcaa acagtccaag cagaggtttt tgctgattcg cttccgtcac cgaattcagt
    20881 gaggcagcat cgctgaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt
    20941 ccctgccatt caccggcggt tgccgtttca gcaaaggttt ttgcgccacc gccaagcgta
    21001 acgtcggcac gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga
    21061 gcgttacccg gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaggtcaca
    21121 tgcgccacca gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc
    21181 gccagacctg cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg
    21241 acgcccagcg cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc
    21301 gaatcggtga cgtagtccgg tttgccggtt tttttattca gcgcatagtg agtgtattgc
    21361 ccggtaagcg gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta
    21421 cgtgcggctg taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt
    21481 gcaggtttat cgctaagaga atcacgcaga gcggcagtct gatcacccgt taaacggcga
    21541 gcaccgccgg atgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct
    21601 ggtgtccggg cttttgtcac aggggtaaac agtaacgata agagtgccag tgcaatagtg
    21661 ctttgtttca ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact
    21721 ataagtctcg gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgacg
    21781 aggatgcttc aataacttct ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat
    21841 gctggcatcg ggctttacct cgtacagcgc cccctctacc tgatcaatca agcgctgttg
    21901 gtcattttgc gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg
    21961 cgcacacagt tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat
    22021 gagatttttc atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca
    22081 caacttcata gtgtctggta gtattatttt tatgacaaag gaaatacttt gcgcaaatat
    22141 tttttgtttg attaccagcg aaacgtttct cttatttaaa taattaccca cacaa
//
LOCUS       DAZFDP010000072        22139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFDP010000072 DAZFDP010000000
VERSION     DAZFDP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..967
                     /gene="focA"
                     /locus_tag="ACHJ1G_002785"
     CDS             110..967
                     /gene="focA"
                     /locus_tag="ACHJ1G_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4764615.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1022..3304
                     /gene="pflB"
                     /locus_tag="ACHJ1G_002786"
     CDS             1022..3304
                     /gene="pflB"
                     /locus_tag="ACHJ1G_002786"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4764616.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3496..4236
                     /gene="pflA"
                     /locus_tag="ACHJ1G_002787"
     CDS             3496..4236
                     /gene="pflA"
                     /locus_tag="ACHJ1G_002787"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4764617.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4318..4908)
                     /gene="ycaK"
                     /locus_tag="ACHJ1G_002788"
     CDS             complement(4318..4908)
                     /gene="ycaK"
                     /locus_tag="ACHJ1G_002788"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO4764618.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5008..5916
                     /gene="ycaN"
                     /locus_tag="ACHJ1G_002789"
     CDS             5008..5916
                     /gene="ycaN"
                     /locus_tag="ACHJ1G_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4764619.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5917..7347)
                     /gene="ycaM"
                     /locus_tag="ACHJ1G_002790"
     CDS             complement(5917..7347)
                     /gene="ycaM"
                     /locus_tag="ACHJ1G_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4764620.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7556..8704)
                     /gene="ycaD"
                     /locus_tag="ACHJ1G_002791"
     CDS             complement(7556..8704)
                     /gene="ycaD"
                     /locus_tag="ACHJ1G_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4764621.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9019..9645
                     /gene="ycaC"
                     /locus_tag="ACHJ1G_002792"
     CDS             9019..9645
                     /gene="ycaC"
                     /locus_tag="ACHJ1G_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4764622.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9681..10544)
                     /gene="dmsC"
                     /locus_tag="ACHJ1G_002793"
     CDS             complement(9681..10544)
                     /gene="dmsC"
                     /locus_tag="ACHJ1G_002793"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4764623.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10546..11163)
                     /gene="dmsB"
                     /locus_tag="ACHJ1G_002794"
     CDS             complement(10546..11163)
                     /gene="dmsB"
                     /locus_tag="ACHJ1G_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4764624.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11174..13618)
                     /gene="dmsA"
                     /locus_tag="ACHJ1G_002795"
     CDS             complement(11174..13618)
                     /gene="dmsA"
                     /locus_tag="ACHJ1G_002795"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4764625.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13918..14910)
                     /locus_tag="ACHJ1G_002796"
     CDS             complement(13918..14910)
                     /locus_tag="ACHJ1G_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764626.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRVRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDARRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAINFNPLAESVHKLSID"
     gene            complement(14980..15321)
                     /locus_tag="ACHJ1G_002797"
     CDS             complement(14980..15321)
                     /locus_tag="ACHJ1G_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4764627.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            15426..15947
                     /locus_tag="ACHJ1G_002798"
     CDS             15426..15947
                     /locus_tag="ACHJ1G_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO4764628.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLNQLRNNRGN"
     gene            15952..16374
                     /locus_tag="ACHJ1G_002799"
     CDS             15952..16374
                     /locus_tag="ACHJ1G_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764629.1"
                     /translation="MKTNTSDFTITISKNDQALIDNLKYLAEKHNNGRIAINLRHITG
                     VTIVTSYWHLYLIAKSVLENKDSVVNGRDVDTLPAQIKPIAYQLRNRFKNEGGIIFDF
                     PAAVAVSFSREDILHSFRFALSGDETLWQLEDAGSSPA"
     gene            complement(16381..16572)
                     /locus_tag="ACHJ1G_002800"
     CDS             complement(16381..16572)
                     /locus_tag="ACHJ1G_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764630.1"
                     /translation="MSEDKKVVNLLSDLSAALDNINPPRVKTTGDKDALNAEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            16708..17058
                     /locus_tag="ACHJ1G_002801"
     CDS             16708..17058
                     /locus_tag="ACHJ1G_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4764631.1"
                     /translation="MKQQRNSSQPRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            17069..17356
                     /locus_tag="ACHJ1G_002802"
     CDS             17069..17356
                     /locus_tag="ACHJ1G_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764632.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            17368..17610
                     /locus_tag="ACHJ1G_002803"
     CDS             17368..17610
                     /locus_tag="ACHJ1G_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4764633.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            17607..17720
                     /locus_tag="ACHJ1G_002804"
     CDS             17607..17720
                     /locus_tag="ACHJ1G_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764634.1"
                     /translation="MSIFISWLVLIISVICAIGIMRIIHSVKKIERFFSDE"
     gene            17814..18224
                     /locus_tag="ACHJ1G_002805"
     CDS             17814..18224
                     /locus_tag="ACHJ1G_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764635.1"
                     /translation="MNAKEKGIITALKNISETANRAIQDATNAGMIGPATTAMMIARV
                     TAEAAEIIEKQDAELTVLRTQSVTELDLSNTGRLIYTIGSEPQQYTIIVGLQDKYLIT
                     PHPIRGSEILTNLRLIERSQVAFIDDAQRTVFNA"
     gene            18249..18452
                     /locus_tag="ACHJ1G_002806"
     CDS             18249..18452
                     /locus_tag="ACHJ1G_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764636.1"
                     /translation="MAIKRFSVIRFTSRGREYEVDERLIKTLDRHRSQPDAHHIYLTD
                     DTYFCATNVVQVNLIRQVQESRR"
     gene            18449..18694
                     /locus_tag="ACHJ1G_002807"
     CDS             18449..18694
                     /locus_tag="ACHJ1G_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4764637.1"
                     /translation="MTILDYITANPGCSGGEIAAALNTPTTAINAELRRLWRDGLVIR
                     EERKTGGRFSYQVNPMPFGCGNPLTHMFNQLLKEARA"
     gene            18691..18990
                     /locus_tag="ACHJ1G_002808"
     CDS             18691..18990
                     /locus_tag="ACHJ1G_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4764638.1"
                     /translation="MSTINHQELRELATDLQRMATHQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCAPLGSPGEPETPEAMQRELLQRIDKVFDFFLNQ"
     gene            19002..19205
                     /locus_tag="ACHJ1G_002809"
     CDS             19002..19205
                     /locus_tag="ACHJ1G_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764639.1"
                     /translation="MNKKTWFRAYMWALVCVLVSLILYAGLLPRMISSDSSFLVLLGI
                     FIAMLYPAGVVRLFSKYIKEIKQ"
     gene            19202..20032
                     /locus_tag="ACHJ1G_002810"
     CDS             19202..20032
                     /locus_tag="ACHJ1G_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4764640.1"
                     /translation="MKKFKLFQIIPLFAAILLVGCDRVEPGNVGIKVNKLGDDKGVGE
                     VVGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTT
                     VFQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQEEMT
                     PIGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAG
                     QADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK
                     "
     gene            20401..20631
                     /locus_tag="ACHJ1G_002811"
     CDS             20401..20631
                     /locus_tag="ACHJ1G_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4764641.1"
                     /translation="MADRKQHRAIAARRHIQTEINRRLFRAFRVAQIMHINMLHERSH
                     ALSNSYSASVFSYLADDLRELQQLIQQQNKLH"
     gene            20704..21069
                     /locus_tag="ACHJ1G_002812"
     CDS             20704..21069
                     /locus_tag="ACHJ1G_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764642.1"
                     /translation="MILANDFLEYLLNTERDLAVRVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHHYADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            21076..>22139
                     /locus_tag="ACHJ1G_002813"
     CDS             21076..>22139
                     /locus_tag="ACHJ1G_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4764643.1"
                     /translation="MTAEYIRDWQQPRHAMGREGTGIPAPESALSSWLDAYRAENERR
                     QEIADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDHRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMEVALKA
                     YQMLARMALHLHTVPPHYEALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKG"
BASE COUNT         5984 a         5725 c         5178 g         5252 t
ORIGIN      
        1 ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat ttctcatcta
       61 taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg tgaaagctga
      121 caaccctttt gatcttttac ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt
      181 ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg ccggtgtttt
      241 catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggca caatgccctt
      301 cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc tttgtgttgt
      361 ctgcggagcc gatctcttta cttccacagt gttgattgtt gttgctaagg cgagtgggcg
      421 catcacctgg ggtcagttgg cgaaaaactg gctaaatgtc tattttggca acctggtcgg
      481 cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg caaatggtca
      541 atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt ttattgaggc
      601 cgtctgcctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga tgagttattc
      661 tggccgtagc ctgatggaca aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc
      721 cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg
      781 cgacttcgca tctccggaat tctggaccgc tgtcggttct gcaccggaaa atttttctca
      841 cctgaccgtg atgaacttca tcactgataa cctgattccg gttacgatcg gtaatattat
      901 cggcggtggt ttgttggttg ggttgacata ctgggtcatt tacctgcgtg aaaacgatca
      961 ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta
     1021 catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca aaggtgactg
     1081 gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacactccgt acgagggtga
     1141 cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca aagtaatgga
     1201 aggcgttaaa ctggaaaacc gcactcacgc gccagttgac tttgacaccg ctgttgcttc
     1261 caccatcacc tctcacgacg ctggctacat caacaagcag cttgagaaaa tcgttggtct
     1321 gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca aaatgatcga
     1381 aggttcctgc aaagcgtaca accgcgaact ggatccgatg atcaaaaaaa tcttcactga
     1441 ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca tcctgcgttg
     1501 ccgtaaatct ggtgttctga ccggtctgcc agatgcatat ggccgtggcc gtatcatcgg
     1561 tgactaccgt cgcgttgcgc tgtacggtat cgactacctg atgaaagaca aactggcaca
     1621 gttcacttct ctgcaggctg atctggaaaa cggcgtaaac ctggaacaga ctatccgtct
     1681 gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa
     1741 atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc agtggactta
     1801 cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac
     1861 ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca
     1921 agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc gcttcctgcg
     1981 tactccggaa tacgatgaac tgttctctgg cgacccgatc tgggcaaccg aatctatcgg
     2041 tggtatgggc ctcgacggtc gtaccctggt taccaaaaac agcttccgtt tcctgaacac
     2101 cctgtacacc atgggtccgt ctccggaacc gaacatgacc attctgtggt ctgaaaaact
     2161 gccgctgaac ttcaagaaat tcgccgctaa agtgtccatc gacacctctt ctctgcagta
     2221 tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta ttgcttgctg
     2281 cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc
     2341 gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg
     2401 tccgaagtct gaaccgatca aaggcgatgt cctgaactat gatgaagtga tggagcgcat
     2461 ggatcacttc atggactggc tggctaaaca gtacatcact gcactgaaca tcatccacta
     2521 catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc gtgacgttat
     2581 ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc tgtctgcaat
     2641 caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg acttcgaaat
     2701 cgaaggcgaa tacccgcagt ttggtaacaa tgatccgcgt gtagatgacc tggctgttga
     2761 cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg acgctatccc
     2821 gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa ctggtaacac
     2881 cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg
     2941 tgaccagaaa ggtgctgtag cgtctctgac ttccgttgct aaactgccgt ttgcttacgc
     3001 taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta aagacgacga
     3061 agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg aagcatccat
     3121 cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat
     3181 ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg cagtacgttt
     3241 caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca ctcaatctat
     3301 gtaattagat ttgactgaaa tcgtacagta aaaagcatac aataaaggct ccacgaaagt
     3361 ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactttaa ggtgactgcc
     3421 aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat gggccacatc
     3481 tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc ctgtggaacc
     3541 gtagacggcc caggtattcg ctttatcacc tttttccagg gctgcctgat gcgctgcctg
     3601 tattgtcata accgcgacac ctgggatacg catggcggta aagaagttac cgttgaagat
     3661 ttaatgaaag aggtggtgac ctatcgccac tttatgaacg cttccggcgg cggcgttacc
     3721 gcatccggcg gtgaggcaat cctacaagct gagtttgttc gtgactggtt ccgcgcctgc
     3781 aaaaaagaag gcattcatac ctgtctggac accaacggtt ttgttcgtcg ttacgatccg
     3841 gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa acagatgaac
     3901 gacgagatcc accaaaatct ggttggagtt tccaaccacc gcacgctgga gttcgccaaa
     3961 tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc aggctggtct
     4021 gacgatgacg attcagcgca tcgcctcggt gaatttaccc gtgatatggg caacgttgag
     4081 aaaatcgaac tcctccccta ccacgagctg ggcaaacaca aatgggtggc aatgggtgaa
     4141 gagtacaaac tcgacggtgt taaaccacct aagaaagaga ccatggaacg cgtgaaaggc
     4201 attcttgagc agtacggtca taaggtaatg ttctaatttc cccgtaaagc ggcaacttta
     4261 ttgagttgcc gcttttttat tccgcgcttc attctgctac agcgttttta acacatttta
     4321 tagtgcatct accatatccc gtacctgaga taataacgac tgataatagc tcgcatgaag
     4381 ttcttgccca tcgaatacca ctgtattaca caagaaagtg acatcggcat cttcaatacc
     4441 aagataactg cacatatttt tcaaataatc gcttatattt ttttcccagc ccatctggac
     4501 aaatgattct ttgtctcctc caaccagcgc cacccaacga actttattga atggtaattt
     4561 gtgcccatct ccataagcca gcccattatt ccatactctg tcaatatatc cttttagcat
     4621 tgccgggaag ctataccacc agagaggaaa aaccaccact aacgtgtcat gttcaagcag
     4681 ctctgaataa agctgatgaa cctctggaga ataacgttta tccatattct tccagtctgg
     4741 ttcatcttcc ggcgtcatca ctgggttgaa attacgccga tataaatcga gttccgtcac
     4801 ctgtattttc cgctccattg cccgctgatg gatcgcatca gcaatatttg cggtcaatga
     4861 atcatgacga ggatgggccc ataccaaata aatacgttca gactgcataa ttttacctcc
     4921 acgttaagtt ctggagcgca ctttacgcag gcggcataat cctgtaaacg tgatgattca
     4981 ggatgagtta atgaaggatt ttcactaatg cggatgaata tgtctgactt tgccactttc
     5041 tttgccgtcg cccgtaatca aagctttcgt gcagcgggcg atgagttagg cttatcctcg
     5101 tccgccatta gccatagtat taaaacactg gaacaacgtc ttaaaattcg cttattcaat
     5161 cggacaaccc gaagcgtatc gttaacggaa gcgggttcta atctctatga acgattgcgc
     5221 ccagctttcg acgaaattca gatcatgtta gatgagatga atgattttcg tctgacaccg
     5281 acaggaacgc tcaaaatcaa tgccgcccgg gtagctgcac gtatattttt aatgccttta
     5341 ctggttggtt tcacacgtga atatcctgat attaaagtcg agctcacaac tgacgattct
     5401 cttgtcgata tcgtccaaca gggctttgac gctggcgttc gacttagtgg catcgtcgaa
     5461 aaagatatga tcagcgtggc gatcggccca ccagtgaaat tatgtgtagc agcaacgcca
     5521 gagtactttg caagatatgg caaaccccgc catcctcatg atctgcttaa tcatcaatgt
     5581 gttgtttttc gttatcccag cggcaaacct ttccactggc aatttgcaaa ggagctggaa
     5641 attgctgtcg caggaaatat cattctggat gatgttgatg ctgaactgga ggccgttttg
     5701 atgggtgctg gtatcgggta tctgctctat gaacagatca aggagtacct tgatactggt
     5761 cgtttagaat gtgtactggg agactggagc acagagcgtc caggcttcca gatctattat
     5821 ccaaaccgcc agtatatgtc ctgcggcttg cgcgcatttc tcgattatgt aaaaacgggt
     5881 cagatctgcc agagtcagcg tcaccgacca caataattat ttactattcg ctttacgcgc
     5941 cagcagcggg aaaatcagtc ctaatcctac cagcacaaac ggcgttgcaa cattcagcgc
     6001 caactggaag gtccactctg cagtgaaggc ttccattttc gggaagatcc ccgtcaaaca
     6061 ggcaaaggcg gtaaaggcaa aacaccaaat cccgacggtc attgccagag gcttattacg
     6121 aataaagaca tattctggtt tatatttctg cgccaggcga acgactgcaa taaatgcaac
     6181 aaatacccat aaataacgca gcggcatcac taccgagtta agattcaaca gccatttata
     6241 gagattgttc atatcaccga tgccgagagt cggcagcata atcagaattg ccaccagcac
     6301 cagggtcaga aaatagccat taacgggcgt accagaagcg ttggtacgac ataaactggc
     6361 tggaatatat ttgctgtcag catcacctaa tagcacttta agcggggcat caatcgagaa
     6421 taccagcgca gctacttgtc ccagggtatt cgcaatggcg taaatcacca ttaaagtatt
     6481 acccatgttg taatactcgc ccagcttctg aaaggcgtaa tattgaccgt tagtcattaa
     6541 gtcatccggg atattacgcg agtcaaacat catccccatc gccagtgagc ccagaatggc
     6601 acaaaccgca accatcaccg ccaggcatag catccctttt ggaaattctt ttcctgggtt
     6661 gcgcgtttga ttaacgtaag gagaaatctt ctctgctccg ccaaccgcga aaaccagcat
     6721 tgaaatagtg gtaatgtagg taaagtcgat atgaggaatg aacgtttccc aggtaatgtt
     6781 tgtggtcgca atatgcactt cagtaatcgc agacgcggtt accgccatcg ccacatacag
     6841 gagcgacata acaaacatcg caatccctgc cacagaaccg acgattttca gcgatttcat
     6901 accgcgtgaa gcaacccaca taaagaagat aaacagcacc agcgttaacc cctgtaacgc
     6961 tacgactgaa tattctttga ttagcgaacc gtcgcctttc atcgcccaac cgagcgcaat
     7021 cagaattgcc tggggttttt gtgccagaaa gggaatatgc accacccagt aggtccacgc
     7081 ggcgagataa gccagtccgg gtcccatcgt gtgtttaatc caggtactga ccccgccctt
     7141 cccatctttg aaggtcgagc ctaactgacc aacaattagc gcataaggtg tgaaatagag
     7201 tgcaaagata aacacccatg aaaaaacaac caccagcccc tggttggcat agttattaac
     7261 aacgttgcca aaaccccaga cagtgataaa agacatcagc gcaatgttgt accatcgcaa
     7321 ctgtttttcc tgaacattcc cagccataga caaatccttc gccaaaatta aaggggcatc
     7381 tgtaaaacga gggattatgc gcggcaaata agaagtgcgg aaatagcgaa atgcaaaatt
     7441 ataacctgct gcacatttta tttttaaacg aataaaattt ggaataaagc aagtcagata
     7501 gaagaaaaaa aaagcagagt gtgaaacagt aagttcacac tctgcttgaa ttcatttaca
     7561 cgtgagcaac gggtttcggc gtatgaccgg cgttgcgcag cagcatcagc aaatagataa
     7621 acgatacgct ggcgatcatg ataaacaata aattatcgga gaaattctgc attagcatag
     7681 cggtaaatga cgggccaagc agacttccca cagtatagct caacagtaag gcctggttca
     7741 tcgccaccag ttgatgatgt tcaactttct cgcaagccca tgccatcgcc accggatata
     7801 gcgtaaagcc agcggcaccg aggatgaata acgctggggc catcgccgcc tggctaagca
     7861 tcgcgatact gccgagaatg acgacaaaga cctgaacacg caacaccagc agtcgaccaa
     7921 acttatccgc cagacgtcca atcggccatt gtccaaggat acccgcactg accagtaccg
     7981 ccatccagaa accaatgctg gcattgctca cccctttgtg attgaggtac agcggcatca
     8041 ggccatatag agatcccaga acgattcctg agataatgca gccattcacg ccaagccgcg
     8101 cctgacggag ttttagcatt gacgtaattg acgtcgaatc atggttttca gcctgctgat
     8161 tcagcacacg cgtaaacaac agcggtaaga tccctgccaa cgtcaaacct gtaacccacg
     8221 gcaatacgga catcagctcg gttgaaactt tgctgaccag taactggcct aaaaacgttc
     8281 ccacgtaata aaccatcata tacgcagcaa gcaaacgccc acggttacgt gacgtcccac
     8341 tgcacatcag cgcgctctca acaaccaccc aaatcatggc acagccgacg cccgcgacaa
     8401 aacgccaagc caaccagctc cagaatccaa tcatcaggcc aaggccggca cagccagcgg
     8461 caaaaatgaa ggaggccaga taatagctgc ggttaaagcc aatgcgctta atgacatacc
     8521 ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga tgagctgaca acgcctacct
     8581 gccatgtgga catgtgttcc tgggcgagcc aaagcggcac gagtgtattt aacaccgcga
     8641 tcgccagagt caacaaaagc aggccagaca gcaaaagcat gacaggccgg gtatacgtgg
     8701 acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc atcatgccac cgccaaaaac
     8761 attgtcaatg ggtgaaaata cgcgcatatc gcagcggcgg tattatcgat ttatattact
     8821 cattcataac gctaatgctg aagagatgat taatctcatc tgattgtttt tattgatcta
     8881 acacaaattt catacgcttc gttcagtcga aaaatttcat ggtcacagca aactcatatt
     8941 tcccgtctat gctttcagag caatgcatca cgtctgaccg atttcagacg taccactgac
     9001 tctcgaaagg agaagaggat gaccaaaccg tatgttcgtc ttgataaaaa tgatgctgcc
     9061 gttttgcttg ttgatcatca ggctggttta ctttcccttg tacgggatat cgaacccgat
     9121 aagtttaaaa ataacgtgct ggcgctgggt gacctggcta agtatttcaa cttacctact
     9181 attcttacca ccagttttga aaccggtcct aacggtccat tagttccaga actgaaagca
     9241 caattccccg atgcacctta cattgctcgc ccgggaaata ttaacgcctg ggataacgaa
     9301 gattttgtaa aagcagtcaa agcgacaggt aaaaaacagt taattattgc cggtgtggta
     9361 accgaagttt gcgtggcatt cccggcgctt tcagccattg aagaagggtt tgatgtcttt
     9421 gttgtgactg acgcgtcagg gacctttaat gaaattaccc ggcattcggc atgggatcgc
     9481 atgtcgcaag caggtgcgca attgatgaca tggttcggcg tggcgtgcga gctgcaccgt
     9541 gactggcgta atgatattga aggactggcg acgttgttct ctaaccatat tcctgattat
     9601 cgtaatttga tgaccagtta tgacacgtta acgaagcaga aataaaagag aaccggggcc
     9661 gaaaccccgg ttgttactga ttagcttgcg acggccatcc ccacggtcat atgcaaacca
     9721 tagaatacgc cgcgaccgat taattcccct gccagaagca gaatgaatga gacggatagt
     9781 agcggaaccg caggctgata accttttagc tgtggtgcaa tccacaagca cagggcaacg
     9841 gccaaaagca cgatccgcca ggacatcagc gcaccatagt ccggcaccag cgctgcggcc
     9901 tgctgcacag aactatgaat agttgccagc tctgtgccct gcatcactga catcacgcca
     9961 ctcaccacca gtgccagtac agaaatggct ggcagcagac gcatcgccca gccatcgacg
    10021 ccagccagac tcaacaacag gtaacccagt aacgggccgc ccataaacat cgtcaggaag
    10081 aagcccatcg gcgtccagat gctgtaccag gtcggaacgg tatcaatgct gttatacaca
    10141 cgcaccatca tccagacaaa gatgacgcca agaaccatcg tcactatcag ccacagcgta
    10201 cgcaatgccg gtgacagctt tttcagcatt gccagcaacc agccgatgcc gcctacggca
    10261 aaaaagatcg aaccgctggc gatttcgtta ctgagtgctg aagcccctac ccggttgagc
    10321 gagttaaaag cgcgcattgg tgaaccaaga tggagcatag aggcgataaa gccaatgccc
    10381 atcagcaccc ataaaccaaa catgcaggcg ataacacgct gctgggcttc tgctcgcagg
    10441 tcgcctttga gcagcgccaa agccagaacg ataaaaccac ctgctacaca ttgcccgaag
    10501 accgtgaaga tcatcagcgg ccattcatgc catccacttc ccatctcaca cctccttcgg
    10561 gtttgccaga tagccagtgg tatccccggt cgggcggcta ttggcattgg gtttgatcac
    10621 aatattcggt ttggtaaagt gagctcgcgg caacggcgca acggccgcca gatcgccatg
    10681 ttttttacgc agctcgtcga tagggccgaa atccagcgcc cgcagcggac aagattcaac
    10741 acagatcggc tttttaccct ccgcaacacg gtcataacaa ccatcgcatt tggtcatatg
    10801 gcctttcgtt tcgttatatt gcggtgcgcc atacgggcaa gccatatggc agtagcggca
    10861 gccaatgcac acatcttcat cgaccacaac aaaaccatct tcacgtttat gcatcgcacc
    10921 gctcgggcag actttagtac aagccggatc ttcgcagtgg ttacatgaaa tcgacagata
    10981 gtaggcaaac acgttctggt gccagacacc gttatcttcc tgccagtcgc cgccagcata
    11041 ttcataaatg cggcggaagc tgacttctgg cgtcaaatct ttgtagtctt tacaggccag
    11101 ctcgcaggtt ttgcaaccgg tgcaacggct ggaatcaata aaaaatccat actgggttgt
    11161 catcggttac tccttacacc ttttcaacct gaacaaggtt tgtatgtgac ggattcccct
    11221 tagcgagagg agacggacgt tgagtggtca gtacgttgat acaaccgccc ttatcgacac
    11281 gttttgtatc cgggtcatac caggcacctt cacccagtgc gaccacaccc ggcatcattc
    11341 gtggcgtcac tttcgcctca atatgaacct caccacgatc gttaaagatc ctgactttat
    11401 cgccgttgtt gataccgcgt ttttgcgcat caagcgggtt gatccacatt tcctgacggc
    11461 aagctgcttt cagcacatca acgttgccgt aagttgagtg aacgcgagat ttatagtgga
    11521 aacccgtaag ctgcagcgga tactgtttgt tcagcggatc ctgataactt tcaaagcccg
    11581 gcgtgtagat cggcagtgga tcgatcacat cgccttctgg caattcccag gtagcggcaa
    11641 tgtcagccag cgcctgcgaa taaatctcaa ttttgcccga tggcgtagtc agtgggtttg
    11701 cctgcggatc ttcacggaag gctttataag caacgtgatg cccttgtggg tcgcgctttt
    11761 taaagatccc ctgcttgcgg aactcttcaa acgttggcag ttcaggaatc gcttcccgcg
    11821 actgggcata cagatgccgc atccactctt cctgggtacg gccttcagta aactgttgct
    11881 caacgccaag acgttttgcc agttcgctgg tcatttcata gatggtctta cattcaaagc
    11941 gcggtttaat cacctgatcg ttgaaaatca cgtaagacat attcccgcag gatgcatcca
    12001 gtgcaaagtc catctgttcg gaagcggtgc agtcaggcag caggatgtca gcatatttcg
    12061 ccgatgaggt catgtggcag tcgataacca caatcagctc gcacttctta tcatcctgaa
    12121 ggatttcatg ggtacggttg atttcagaat gctggttaat caggcagtta ccggcatagt
    12181 tccagatcat tttgatcggc acatccagct tatctttccc gcgtacacca tcacgcagcg
    12241 ccgtcatttc cgggccacgt tcaatggcat cggtccacat aaacatcgaa atgctggtct
    12301 ggatcgggtt ttccaaggtc ggcatacgga caaacggtaa gctgtatgaa ccttcacgcg
    12361 cgccgctgtt gcctccgtta ataccaacgt taccggtcag aatcgccagc atcgagatag
    12421 cacgggttgc gatttcaccg ttagcgtgac gctgcgggcc ccatccctgg ctgataaacg
    12481 ccggtttggt actgccgatt tcacgagcca gtttgatgat tttgtctgcc ggaacaccgg
    12541 tgatttgtga ggcccattcc ggtgttttag ccacgccatc tggcccttca cccagaatat
    12601 aagctttata gtggccattt ttcggcgcac tggctggcag ggttttctca tcgtagccaa
    12661 cgcaatattt atcgaggaat gcctgatcca ccaggttttc agtgatcatg acgtacgcca
    12721 gaccgttaac cagtgcggca tctgttcccg gacgaatagg gatccactca tcttcgcgcc
    12781 cggcaccggt gtcggtatag cgcggatcaa tgatgatcat gcgggcatta gatttctggc
    12841 gtgcctgttc aagatagtaa gtcaccccac cgccactcat tcgcgtttcg ccagggttat
    12901 taccaaacag cactaccagc ttactgtttt cgatatccga cgggctgttg ccatctgccc
    12961 agccgccgta ggtatagttc aaaccttccg caatttgcgc ggaggagtag tcgccgtaat
    13021 ggttcagata gccgccgcag cagttcatca gccgcgcgac cagggtattt cccggcggcc
    13081 aggagcgggt catggtgccg cccagcgtac cggtgccata gttcagatag atagactcgt
    13141 tgccgtactc tttgatcagg cgctgcatat tggtcgcgat gatgtcgtag gcttcttccc
    13201 agctaatgcg ctcgaatttg ccttcaccgc gcgccccgac tcgtttcatc ggatatttca
    13261 ggcggtccgg attgtagaca cggcgacgca tggaacgccc acgcaggcag gcgcgaacct
    13321 ggtgcaggcc gtcgtaattg tcatcgccgg tattgtccgt ttcgacatat ttgatttcac
    13381 cgtccacgac gtgcatacgt agcgggcagc gactaccaca gttaactgta caggcgctcc
    13441 agataacctt ttcgtctgat tttgttggaa tggcgctatc gacagcgtgc gcaatccgac
    13501 taaaaggtaa tgttaatgcg ctgctggcca ttgccaggcc gccgatcgct gtcgttttta
    13561 ccaaaccacg gcgactcacc tcagcagcca ataccgcatc agggattttc gttttcataa
    13621 tggctcactc aagcttgctc acgattgatt ccagtaacaa accgtattgt gtaattattt
    13681 ctatagcagc tcgctatata ggcttgtata catcgaataa tttgaaaatc aaagagatag
    13741 gtagattttc actcgaaagt agtattacgt gtaagtgagg agtaattatt gttcggtatc
    13801 aaagggagac aaaaaaagcg cccgcaggcg ctttaacgtt tcttgctccc cctgatgatt
    13861 tacttatttt tcaacgaata agccaaaaca gggggaacaa attaacacaa attataacta
    13921 atcgatggac agtttatgga cactttcagc cagcggatta aagttgatcg cgtcctgtaa
    13981 aaagtccggc gaaaaatgag cgtacgtcat tgtttgctga atcgttgcgt gccccaggat
    14041 tcgttgcaac gtaacaatat ttccgccatt catcataaag tgtgtggcaa atgtatgacg
    14101 catgacatgc gtagcctgcc ccttcggcaa atccggtttc acctcctgaa gaatctcccg
    14161 gaacttcaga taatcaacct gatacaataa ccccgttcga cgggttttaa tcagaggcac
    14221 gaccgaatcc gccacaggaa ccgaacggga ttttccgttt ttggtgttaa agaacgtcac
    14281 ccggttgcca acgatatgct ctccacgtag ttccgatgct tcactccatc gggcaccagt
    14341 agaaagacac agaatggcaa cacgacgcgc atcaccctcc agtcgctcaa gaagccgttc
    14401 aatctcatca tcagaaagaa aggccatctc tgtgttctga actttcagtt ttcgtatacc
    14461 gcgtacaggg ttcgcgttgt gaaagacctc agcttcaatc agcaccgtga acatggtaga
    14521 cagtacgcat aagtcacgat taatactgga cggcattaat ccggcctgta atttctctga
    14581 ccgatattca agcataaatt ttcgcgtcat ctgactggcg cgaggatctc ccatttcacg
    14641 aacaaccttc cctagcctaa ccctgtagct atccccgtaa gcctgattac gcccatcaag
    14701 catccaccac gcatcaagca gctctgataa tcgccgctga tcagctggct tagcctgcca
    14761 tggcttgtca tgaaagttct gtaacacata cttctcaaac tcctgagcct ttgatttcag
    14821 ggcaaagatt tttcgaaccc gctttccttc cgccccctgc ggtctgacat cgacctgata
    14881 acgtccgtct ttgagttgct taatcgacat gattagcccc tccaacggat aaattaacta
    14941 aacactcctt gcgatatgta attcgctcaa tgtgtagagt cagccaacat tgcggcctga
    15001 tcggggtgat ttttgggtaa taccgattga aacctgatcg ccctccgact gaatagatcc
    15061 atcaagagag agagccggag caatctgccc ggcgacaggt agcgtcttat cttttattag
    15121 ccacagcatg tatttttcaa agcgatcgac ttgcagaacc gcctcaacga tttttgctct
    15181 agcgttcttt tgccccgatt cgtaattcct tatggttgcc aaagacaacc cagtaatatc
    15241 cgcaaactgc ttttgtgtta acccttccgc cttacgtatc tgacgtaact tttttgcata
    15301 gtctattgac aagataacca tttgagtatt aacctctcgc acaaagataa ccatttgatt
    15361 atctttacaa gccaaaacaa accatcacaa ggcgcgacaa gcgcaccaac gcaagaggat
    15421 aacaaatgcg ggacttcatt gccgataaag agttatctga ccaccaacca cttcaggaaa
    15481 gtgacgcact cagcggatcc attgatgccg ccaacgacga cgaacacgaa gaaaacaaga
    15541 aaaagcgttc ctacaaaaaa ggagcgactt tacggctgga tggtccgatc gccgggcttt
    15601 gctctctgga aaaaggcgcg gcatatattg ggctaacaaa atctgcactg cgcgtagcca
    15661 ttcatcgcgg tcaaatgccg ggacacaaga cgcgcacaaa cccggaagat gaaaactcag
    15721 acggagtatg gtggtttaac gccaaagagt gggacaagtt ggctgatgag ctaccagagc
    15781 acgagccccc agaatggcac aactggaaaa gttactggac gtatgaccgc cagaaaagga
    15841 agttctctcc agctaataaa gaagactgcc agaccattaa cggtaagcga gtttatacag
    15901 gcagaagctc aaaactgaac caactcagaa acaacagagg caactaatcg tatgaaaaca
    15961 aacacgtcag atttcacaat aactatttca aaaaatgacc aagcattaat tgataacctg
    16021 aaataccttg cggaaaaaca taataacgga cgaattgcaa ttaatttacg acacatcaca
    16081 ggagtaacaa ttgtaaccag ttactggcat ctatatctaa tcgctaaatc agtactggaa
    16141 aacaaagatt ctgttgtcaa tggaagagat gtagatactc ttcctgcaca aatcaaaccg
    16201 atagcctatc aattgcgtaa tcgttttaaa aatgaaggtg ggattatatt tgactttccc
    16261 gcagctgtag ccgtgagctt ctctcgggag gatatacttc actcatttcg ttttgctcta
    16321 tcaggtgacg agacattatg gcagctagaa gatgccggga gcagcccggc ataaatcaaa
    16381 ttaaaaatca accagttgtg tgacaggtgg gtatttacgc tgaattttgt cagttactaa
    16441 cgccagtaat atatgaatct ctgcgtttaa tgcgtcttta tcacccgtcg tttttaccct
    16501 aggagggtta atgttatcca gagcagcact aaggtcactc aacaaattaa caactttttt
    16561 atcttctgac atgaaaacct ctaaacaatt taattaaaca taagccagga cctgaaccgg
    16621 ccagtccaag tcctagacac acaaaaaata ccacaaatca atacaaggtg acacgagtac
    16681 accttaccga aggtacagag ttttaatatg aaacaacagc gcaattcttc acagccgcgc
    16741 ttccgcaacg gtgcggaacg ccacgctaac cgtttcgcta ccagtgcatc acgtagcaac
    16801 tctcgctaca gcctgagcga aacacacgca acgccggatg gctacccagt aaaacaaatc
    16861 ggcgagcata cctggctgat tgagaaagct ggaattgtgg tccacaaatg cccacgcaat
    16921 ccgtttaccg gaaaccgcat ttttgcattg agctgtggcg acaatcagtt tgggcaggat
    16981 ttcacattat acgaagcact tcgcacggtt gatcgtctgc ttcgcgggca aagttttatt
    17041 aaacaggctg atttataacg ggtgctttat gaccaaagac catgcacaag gtgtatttat
    17101 ccgttttatt gattttcgcg gtgaactgtt attacgtgca tccgctattg acggagtgac
    17161 tccggcgggt aaaaacggag ccgacgaagc cacttacgtt tatctgaacg gcacgcgact
    17221 gcttgtggaa cttccgtacc agaccgtacg agaaatcatt agcgaagctg aaaaggcacg
    17281 ccaagttaat ggcgatgaac cctatatcga aattatttgt atggattcag aagctgaaat
    17341 tcagaaagca gattaaaggg cgttgcgatg ggcaaagaat ataaaactct cattaataaa
    17401 gcacttgagc gtttttattt tcgcttaagt gcatcaggcg ctcatgctga acgtgcagcc
    17461 cgtgactcat tgaccagggc aatccggagt ctgtatgacg tggcttttta cgctgatgat
    17521 ctggatgcac ttaacgaact ttccgagctg atctgtgccg cagaatgcgg ggaacatatt
    17581 gaaccgtata agctggggaa tatcgcatga gtatatttat ctcgtggctt gttctgatta
    17641 tttcggtgat ctgcgctatt ggcattatgc gaattattca ttcagtgaaa aagattgagc
    17701 gttttttctc tgacgaataa cagcacaaat aaaacaccag attaaataag aaaacgtgaa
    17761 aaccatccga attaacggag gcattcacac acgtaaataa tggagataca aaaatgaacg
    17821 caaaagaaaa aggcattata actgcgctaa aaaatatatc ggagaccgca aacagagcca
    17881 ttcaggatgc aacaaatgca ggaatgattg ggcctgccac cacagccatg atgatagcaa
    17941 gagtaaccgc cgaagcagcc gaaattatcg aaaaacagga tgctgaacta acggttctca
    18001 gaacacaatc agtcaccgaa ctggatttgt ctaacaccgg acgccttatt tacacaattg
    18061 gctcggagcc acagcaatac accattatcg tcggattaca ggataaatac ctgatcactc
    18121 ctcaccccat aaggggatca gaaattctga caaatctccg cctgatagag cgctctcaag
    18181 tcgcattcat tgatgacgca cagcgcaccg tatttaacgc atagggttac tggacaaagg
    18241 ggggcgcaat ggcaattaag cgtttttccg tcattcgttt cacatccaga gggcgtgaat
    18301 acgaggttga cgaacggctg attaaaacgc tcgaccgtca ccgttcgcaa cctgacgcgc
    18361 atcacattta cctcactgac gacacttact tctgcgccac caacgtggtg caggtgaatc
    18421 ttatcagaca ggtacaggag tcacgcagat gaccattctg gactacatca cagccaatcc
    18481 gggttgtagc ggtggagaaa tcgccgccgc gctgaatacc ccaaccacag ccattaatgc
    18541 tgagttgcgc cgactctggc gtgacggctt agtcatcaga gaagagcgca aaacaggcgg
    18601 tcggttctct taccaggtaa acccgatgcc gttcgggtgt ggcaacccac ttactcacat
    18661 gtttaaccag ctactgaagg aagccagagc atgagcacca tcaaccacca ggaactacgc
    18721 gaactggcga ctgacctgca acgaatggca acgcatcaaa aattactggc gtttcgcgca
    18781 atgctctcgc catctgccgt gttggcacta ctggatgagc tggagcacgc cagaaccaca
    18841 gcgccagcca ttcgcctgac gctccatcat gaaatcgctg atttctgtgc accactgggt
    18901 tcacctggcg aaccggaaac accggaagca atgcagcgag agctgctgca acgcatcgac
    18961 aaggtttttg attttttcct taaccagtaa gggatcgcag catgaacaaa aagacctggt
    19021 ttcgcgcata catgtgggcg ctggtatgcg tcctcgtttc tctcattctg tatgcaggac
    19081 tcctcccccg aatgatttca tcagacagct ccttcctggt attgctgggc attttcattg
    19141 ccatgctgta cccggcaggc gttgttcgcc ttttcagtaa gtacatcaag gaaatcaaac
    19201 aatgaagaaa ttcaaactct ttcagattat ccctcttttt gccgccatcc tgctggttgg
    19261 ttgcgatcgt gttgagccag gtaatgtggg catcaaagtc aacaaactgg gcgacgacaa
    19321 aggcgtcggt gaagtggttg gtgttggccg ctactggact ggctggaata ccgaggttta
    19381 catcttcccg accttcaaac aaatgaagac ctacgatgag ccgttcagct tccagatgag
    19441 tgacggcaca accatcggct atcacatcgg tgtggcctac aaggttgatc catccaaagt
    19501 tactacagtg tttcagacct accgcaaagg cgtggatgac attaccgaca ctgacctgcg
    19561 ccagaagatc gccgatgcac tcaaccgact ggccagcaaa atgaccaccg ataagtttat
    19621 cgacggtggt aagtctgaac tgctggattc agcacttaaa gatattcagg aagagatgac
    19681 acccatcggt attcaggtaa tgagcctctc ttatgtcggt aaaccggaat atccgccaac
    19741 cgttatcgac agcattaacg ccaaagtcac ggcaaaccag aaaaccctgc aacgcgaaca
    19801 ggaagtcaag caacgcgaag cagaagccaa catgttgcgc gcagaagctg ccggacaggc
    19861 tgatgccatt cgcacaaaag cccaggctga agccgacgcc attcgtttac gcggcgaagc
    19921 tctgcgccag aatcccggcg ttatggagct ggaagcaatc aacaaatgga acggcacgct
    19981 gccgcaatac atgaccagca acaccgctgt tccgtttgtt ccggtgaagt aattaaaccc
    20041 ggccagtgaa aatcgctggc cggagcagta tcaggatttt tttagtatgc cgttctcaca
    20101 aaaaaaccgc ttgccatgcc gcaatcagtc aggttacatt tccgctgcac ctcataaaac
    20161 gggtgccggg attctcaacc cgatacagag caaagcgcat aaccgcgcaa gcggtttttt
    20221 tgtgcgtact gtattgccac gtctttttcg cgtcagaatt atggcggggc gtacggggcc
    20281 gacttcggtc gggccggatt ctttgctctc cggtgttgag aaccctgtac gtctcgccac
    20341 cccgagattc tcaactctgg atggtgagtt atttctatca ccgagcaaag aggccacacc
    20401 atggcagacc gcaaacagca ccgcgctatc gctgcgcgtc gtcacatcca gactgaaatc
    20461 aaccgcagac ttttccgcgc attccgcgtc gcgcaaatca tgcacatcaa tatgctgcat
    20521 gaacgcagtc acgcactatc gaacagctat tccgcctctg ttttcagcta tctggcggat
    20581 gatctgcgcg agcttcagca gctcatccag cagcaaaaca aactccatta attcctgttc
    20641 cgggcctttc ctgcaccttg cggcgggagg ccttcgcaca tctgtaacaa gaggagtgcc
    20701 gcaatgattc tcgccaacga ctttcttgaa tacctgctca acacagagcg tgatcttgcc
    20761 gttcgcgtgc gtgaccgtta tgacatgtac ctgaaatccc tgcctgtacc gcagctcgct
    20821 gacggaaaga ttgttattga tggccgctac atgattgaca gccacgaggg aaattacagg
    20881 ctttaccgca ttgaaggcgg caccccgtcc gttattggca tttaccagcg cccatcctct
    20941 gcgatcgtcg atgtgattgc agacagcatc cgcatcacac atcactatgc cgacacagaa
    21001 gacaccgtgc tggaaattca gcggctggct acagtctgcc gcgacaccct gaatggcatg
    21061 acgaagtaaa tcactatgac agcagagtac atcagggact ggcaacaacc gcgccacgca
    21121 atggggcgtg aaggaacggg gatccctgct cctgaatccg cgctttcctc ctggctggat
    21181 gcctaccggg cagagaacga gcgccgccag gaaatagctg atgcggcgtt ctccgccaca
    21241 ccgctgggca acctgattaa taaaagcctg gacgcacagg aaaaacagga caaaaccatc
    21301 acactggcag gagacgccag aaaacaggca cgcggcgcgg tggatgaagc catggcctcg
    21361 ctgcgcctgc tgccgtccta tctgcgcgat ccgcttattc gccacctctc cttcctgcgc
    21421 aaaaaacagg aagccgatcg ccggaaaggc aaaaagagct ggcaggcgga acgctatgca
    21481 cgcggaaccc tgcgcaaaat attcgaacgt ctggaccgca ccgatcaccg ctggctgaca
    21541 ccgggttatc gttcccttgc cggacgcgaa cgcctggacg atttgcttta cctgccgcag
    21601 ctcaacaaac accagataca gacgctggcc accatgacgg cggcgatgtt cagcagcacc
    21661 ttcgaaaaac tctgcgatgg ctttggcgcg actgatggtg agctgaccat ggaggtggca
    21721 ctgaaggcgt atcagatgct ggcccgcatg gcgttacacc tgcacaccgt gccgccgcat
    21781 tacgaagcgc tgacaacaga caaagaccgg aggaacgaac cggacacgga gctgctgccg
    21841 ggcgcaatcc ttcgcctgac ctgtgcggaa tggtggaaac gcaaactgtg gctgttacgt
    21901 tgcgagtgga gagaagaaca actccgcgcc gcctgtctgg tttccagaaa aacatcgccc
    21961 tatctgagcc aggacgcgtt aagcgagttt cgcgcacagc gcgagaagac acgcgatttc
    22021 ctgaaaagtt tcatgctgga aaacgaagac gggttcacga ttgatctcga gacagtgtat
    22081 tacgcgggag taagtaaccc ggttcaccgt aaggcagaaa tgatggccac catgaaggg
//
LOCUS       DAZFDP010000073        21723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFDP010000073 DAZFDP010000000
VERSION     DAZFDP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..1385
                     /locus_tag="ACHJ1G_002814"
     CDS             213..1385
                     /locus_tag="ACHJ1G_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4764644.1"
                     /translation="MSTEPKETTASVRMSVAEKRSAEIRAKSLGYKSLSDYLRALASS
                     DMKSATPDPKKCRETRPVSLFHKTNFGAMWNGDSLDWMDSREAESVNLIMTSPPFGLV
                     RKKSYGNEDAHAYCDWFRPFAEGFKKILKDDGSLVIDIGGAWKAGTPTRSLYHYELLL
                     MLCNEYGFHLALEHFWWNPAKLPTPAEWVNVRRVRPKDAVNCVWWLSKTPFPKASNRR
                     VLAPYSDSMKSLLKNGYQAKTRPSGHVISEKFGKDNGGSVPPNLLAIANTESNGTYQD
                     YCRKKGLDIHPARFPSALPEYFIRMTTDPGDFVFDPFGGSCVTGAVSEALGRHWACVE
                     MNSAYLEGAMSRFEGDPIVLATDKPSSYTIFTPCAAQVVEEAVPLVADGGAARPKK"
     gene            complement(1387..2610)
                     /locus_tag="ACHJ1G_002815"
     CDS             complement(1387..2610)
                     /locus_tag="ACHJ1G_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764645.1"
                     /translation="MNSMSLDEICLRGLLRWVGTGSPSPYSGSVETLRYEDLDALRMW
                     WALSEPVGRLALRLVEKQREVSPTLDDFRREVSGELPGPPDAAASMLLQTVTLDPAAF
                     VVMEASSSWFSGPNRVLAKTLKTAQVALKAGALHARGGLFDLHANKRLALLDCALKVA
                     PIREVLSTPAGRSRIVAYEKRQASKARSPLYKQSWLCASILSDIEAFKADTIKALLAS
                     DLLPGMEVWRKFELATILEVAEALSGATGAPVVLDASFAAGRPAATIGDLELRWQRAM
                     PKRPSAFLDPGEVLAGELAFSLGVSKGTSRADITIERAGRILSIIECKWFSSPDSAPA
                     AILEATSQLVGYARDIAHAQEESDTGILARSIVALAHRGPAEMHFGAPVCCIGFSDFG
                     TANLAPWAAMVSGIH"
     gene            complement(2610..4298)
                     /locus_tag="ACHJ1G_002816"
     CDS             complement(2610..4298)
                     /locus_tag="ACHJ1G_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4764646.1"
                     /translation="MATSLTTQDILGLFKALKDDIQATILKPRGLSIKSSGTKSLVLN
                     EPLNPETGFWQNPQAMSNKPLRWIPQVEVSAENVVARFAKNVDGQSIGMAKSVGYINN
                     RDGSCSVCVFRVLPAIKPLSPDSNWEFPAEWEFLLILGFAQPLPAGTSGDPLSNFEPS
                     EGTIDFGGGALKRTFPISFRALVKAPVSEEYVAGTSYSVVGNAIARLVGATVYDGTQA
                     SGRDALVTDLSNIFAPQSVEGPFSLDENTSLVGIEPSVYQQIEAAINSGKRHIILYGP
                     PGTGKTTLAEYLAAEVSERDDGDGAYLMLTASSAWSSQDLVGGYQPLGNGAIGFVPGA
                     LLRNFDKPVIIDELNRCPIDKVLGPLFSIFSGQSSTLPCRVEVANPASPFHVIYPKPH
                     PGMALHEHAPEPAWRMICTLNTYDKTQLGQISYALSRRFAWIKIGAPADPDGFVREMA
                     TRLGATVTEPVASNPIGALWKAVNSLREIGGAPIVDFIKTMLAFKSDVNLFVQPEGNT
                     ADLFLSAFRMCVLPLMDGLSPREARDLAESISVAWGLDAEKAASLTRDCAEFSA"
     gene            complement(4538..4840)
                     /locus_tag="ACHJ1G_002817"
     CDS             complement(4538..4840)
                     /locus_tag="ACHJ1G_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764647.1"
                     /translation="MKLLNIYEDKDEAEVALSKISGEKRLASERDGTVTIYNLFGQPT
                     WGNFYKLDLFNLRELQEILLLRKEGKPIDQKRYEEIIITLRYVSRSFDLSIPEHWL"
     gene            complement(4885..5331)
                     /locus_tag="ACHJ1G_002818"
                     /pseudo
     CDS             complement(4885..5331)
                     /locus_tag="ACHJ1G_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5437..6618)
                     /locus_tag="ACHJ1G_002819"
     CDS             complement(5437..6618)
                     /locus_tag="ACHJ1G_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4764648.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTLHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQTTDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            complement(6795..6870)
                     /locus_tag="ACHJ1G_002820"
     tRNA            complement(6795..6870)
                     /locus_tag="ACHJ1G_002820"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6835..6837),aa:Phe,seq:gaa)
     gene            complement(6976..7683)
                     /gene="yqgA"
                     /locus_tag="ACHJ1G_002821"
     CDS             complement(6976..7683)
                     /gene="yqgA"
                     /locus_tag="ACHJ1G_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4764649.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            7836..7919
                     /gene="yqgH"
                     /locus_tag="ACHJ1G_002822"
     CDS             7836..7919
                     /gene="yqgH"
                     /locus_tag="ACHJ1G_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO4764650.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            8081..10216
                     /gene="speC"
                     /locus_tag="ACHJ1G_002823"
     CDS             8081..10216
                     /gene="speC"
                     /locus_tag="ACHJ1G_002823"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4764651.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(10266..11522)
                     /gene="nupG"
                     /locus_tag="ACHJ1G_002824"
     CDS             complement(10266..11522)
                     /gene="nupG"
                     /locus_tag="ACHJ1G_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4764652.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11724..12803)
                     /gene="mltC"
                     /locus_tag="ACHJ1G_002825"
     CDS             complement(11724..12803)
                     /gene="mltC"
                     /locus_tag="ACHJ1G_002825"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4764653.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(12868..13143)
                     /gene="yggX"
                     /locus_tag="ACHJ1G_002826"
     CDS             complement(12868..13143)
                     /gene="yggX"
                     /locus_tag="ACHJ1G_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4764654.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(13171..14223)
                     /gene="mutY"
                     /locus_tag="ACHJ1G_002827"
     CDS             complement(13171..14223)
                     /gene="mutY"
                     /locus_tag="ACHJ1G_002827"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4764655.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            14384..15103
                     /gene="trmB"
                     /locus_tag="ACHJ1G_002828"
     CDS             14384..15103
                     /gene="trmB"
                     /locus_tag="ACHJ1G_002828"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4764656.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            15103..15429
                     /gene="yggL"
                     /locus_tag="ACHJ1G_002829"
     CDS             15103..15429
                     /gene="yggL"
                     /locus_tag="ACHJ1G_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4764657.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            15613..16332
                     /gene="yggN"
                     /locus_tag="ACHJ1G_002830"
     CDS             15613..16332
                     /gene="yggN"
                     /locus_tag="ACHJ1G_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4764658.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            16508..17554
                     /gene="ansB"
                     /locus_tag="ACHJ1G_002831"
     CDS             16508..17554
                     /gene="ansB"
                     /locus_tag="ACHJ1G_002831"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4764659.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            17671..18678
                     /gene="yggM"
                     /locus_tag="ACHJ1G_002832"
     CDS             17671..18678
                     /gene="yggM"
                     /locus_tag="ACHJ1G_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4764660.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(18734..20035)
                     /locus_tag="ACHJ1G_002833"
     CDS             complement(18734..20035)
                     /locus_tag="ACHJ1G_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO4764661.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(20035..20538)
                     /locus_tag="ACHJ1G_002834"
     CDS             complement(20035..20538)
                     /locus_tag="ACHJ1G_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO4764662.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(20583..21569)
                     /locus_tag="ACHJ1G_002835"
     CDS             complement(20583..21569)
                     /locus_tag="ACHJ1G_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO4764663.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         5815 a         5187 c         5264 g         5457 t
ORIGIN      
        1 atacggcggt cggtttacgc ttacgaaaat tctccactgt catagtgaca attacaaaaa
       61 tcttcgtttc aaaacgaatt tggcaaagaa aggccttgcg attgatgtta agtggggaga
      121 aaatgacatc attaagtaaa tacgtttaac tgccttattt ttgtattaca aagaaaggaa
      181 attttaagtt ggagaagaac atggtacgcc caatgtcaac agaacctaag gagacgactg
      241 cctcagtgcg aatgagcgtt gctgaaaaaa gatcggcaga aatcagagct aaatccttag
      301 gctacaaatc gctaagtgac tatcttcgag cattggcgtc ctcagacatg aaatcggcaa
      361 ccccagatcc gaaaaaatgc agagaaacac gccctgtatc tctttttcac aaaacaaact
      421 tcggtgcaat gtggaatggt gactctctgg attggatgga ttcacgtgaa gcagaaagcg
      481 taaaccttat tatgacctcc ccacctttcg gtcttgtccg gaaaaaatcc tatggtaatg
      541 aggacgcaca cgcttattgt gattggtttc gcccatttgc tgagggtttc aaaaaaatac
      601 tcaaagacga tgggagctta gtgattgata tcggaggcgc atggaaggca ggaaccccca
      661 cacgctctct ataccattat gaactgctgc taatgctttg caatgagtac ggttttcatt
      721 tggcgctgga acatttttgg tggaatcctg ctaagttgcc gacacctgca gaatgggtaa
      781 atgtaaggcg ggtacgacct aaagatgccg taaattgtgt atggtggctt tcaaaaacac
      841 cattccctaa agcaagtaac aggcgcgtgc ttgcaccata ttccgactca atgaagtctt
      901 tactaaaaaa cggttatcaa gcaaaaactc gtccctcggg ccatgtgatt tctgaaaaat
      961 tcggcaaaga taatggtggt tccgttcccc caaatctcct tgctattgcg aatacagaat
     1021 caaatggcac ttatcaggat tattgtcgca aaaaaggatt ggacatccac ccagcgcgtt
     1081 ttccatcagc gcttccggag tattttattc gcatgactac ggatcctggc gattttgttt
     1141 tcgatccatt cggcggctct tgcgtaacag gtgcagtgtc tgaagccctt ggccgccact
     1201 gggcttgcgt agaaatgaat tcagcatacc ttgagggcgc aatgtcccgg tttgaaggtg
     1261 atcctatcgt tttagctaca gacaaaccat cctcttacac aatttttaca ccatgtgctg
     1321 cacaggtagt ggaagaggct gtccctttag tagctgatgg tggggccgct cgtccgaaaa
     1381 aataaattag tgtattcctg ataccatcgc tgcccaaggt gccaaattcg cggtaccgaa
     1441 atcagagaaa ccaatgcagc aaacaggggc tccaaaatgc atttcagcag gccccctgtg
     1501 agcaagagcc acaatgctac gagctagaat acctgtatca gattcttcct gcgcatgcgc
     1561 aatgtctctt gcataaccta caagctgact agttgcctca agtattgctg ctggtgccga
     1621 atcgggagaa gaaaaccatt tacattcaat aatcgaaaga atacgtccag ccctttcaat
     1681 agttatatct gctcgagaag tacccttgga aaccccaaga gagaaggcca gctcacctgc
     1741 tagcacctct cccggatcta aaaacgcact tggtcttttg ggcatggctc tttgccaacg
     1801 taattccaaa tcaccaatag ttgccgctgg cctgcccgcc gcaaacgaag cgtcaagaac
     1861 aacgggagcc cctgtcgccc ctgaaagggc ttcagcaact tccagaatcg ttgcgagttc
     1921 aaattttctc cagacctcca ttccaggaag taaatcggag gcaagtaggg ccttaatcgt
     1981 atcagcctta aaagcttcaa tatctgataa tattgatgca caaagccaag actgcttata
     2041 caacgggctc cttgccttgg aagcctgcct tttttcataa gcgacaatac gagatcttcc
     2101 cgcaggtgta ctaagtactt ctcgtatagg ggcgactttt agggcgcaat cgagaagagc
     2161 taatctttta ttcgcatgca aatcgaaaag acctcctctg gcatgtaaag ctcctgcttt
     2221 caaagctact tgcgctgttt ttagcgtttt cgcaagaacc ctgtttggcc ctgaaaacca
     2281 tgaagaagat gcctccatga ccacaaaagc tgcaggatca agagtgacgg tttgaagtaa
     2341 catagaagct gcagcgtcag gagggccagg aagttcacca gatacctccc ttctgaaatc
     2401 gtctaaggtg ggggaaactt ctctttgttt ttccactaat cttaaggcta gcctgccaac
     2461 aggttcagaa agagcccacc acattcttaa agcatctaaa tcttcatatc ttagagtctc
     2521 cacgcttccc gagtagggtg aaggagaccc tgttcctacc cagcgaagaa gaccgcgaag
     2581 acatatttca tcaaggctca tggagttcat cacgcagaaa actccgcaca atcacgagtt
     2641 aatgaagcgg ctttctcagc atctaggccc catgcaactg aaattgattc tgctagatcc
     2701 cttgcttctc ttggcgacaa accatccatc aatggaagaa cgcacatgcg aaacgcggat
     2761 aaaaacaagt ctgcagtatt accctctggt tgtacaaata ggtttacatc agatttgaac
     2821 gcgagcattg ttttgatgaa atcgacgata ggggcaccac ctatttcacg taatgaatta
     2881 acggccttcc acaaagcgcc aatcgggttt gaggctacag gctctgtaac tgtagcgcca
     2941 agacgagttg ccatttccct tacaaatcca tcaggatctg ctggcgctcc tattttaatc
     3001 catgcaaaac gacggctcag tgcataggaa atctgaccaa gctgggtttt atcataggta
     3061 ttcaaagtac agatcatacg ccatgcaggc tctggtgcat gctcgtgaag tgccattcct
     3121 ggatgaggtt taggataaat cacatgaaat ggacttgcag ggtttgccac ctcgacccga
     3181 caaggcagtg tcgaagactg tcctgagaaa atcgaaaaca acggtcccaa aactttatca
     3241 atcggacaac gatttaattc gtcaatgatt acaggtttat caaaattacg gagcaatgca
     3301 ccaggaacaa agccaatagc cccattacct agaggctgat acccgccgac aagatcttga
     3361 gaactccaag cagacgatgc tgtgagcatt agataagctc catcgccatc atccctctct
     3421 gaaacttccg ctgcaagata ttccgctaac gtcgttttac ctgtacctgg aggaccatat
     3481 aaaattatat gacgcttacc agagttgatt gctgcttcta tctgttgata taccgacggc
     3541 tcgatcccaa caagagaagt attttcatcg agtgaaaatg gtccttcaac tgactgagga
     3601 gcaaagatat ttgaaagatc agtaactaag gcatcacgtc ccgatgcttg agtcccatcg
     3661 tatactgtag caccaactaa ccgtgcgatt gcattgccaa cgactgaata tgatgttcct
     3721 gctacatact cttctgagac aggggctttc actagggccc ggaagcttat cggaaatgtt
     3781 ctctttaatg cgccgccacc gaaatcaata gttccttcac taggttcgaa attgcttaat
     3841 ggatcacctg acgtgcctgc gggcagaggc tgcgcaaagc caagaattag caggaactcc
     3901 cattccgcag gaaattccca atttgaatca gggcttagag gtttaatcgc agggagaacg
     3961 cgaaaaacgc atacagagca agagccatcg cggttgttaa tgtaaccgac gcttttagcc
     4021 atacctatgc tctgcccatc aacattttta gcgaatcttg caacaacatt ttcagcgcta
     4081 acttcaactt gaggaatcca gcgtaggggc ttatttgaca tcgcctgcgg attttgccaa
     4141 aagccagtct caggattcaa tggttcgtta agcacaaggc tctttgtacc cgagcttttt
     4201 atcgacagtc ccctcggctt caatattgtt gcctgaatat cgtctttcaa cgccttaaac
     4261 agcccaagta tgtcttgcgt cgtcagcgag gttgccataa tgttcccttc tttgtctgcg
     4321 tttttatctc cggatacaac aattttccat ctccttacct ttaggtcaac catataccgt
     4381 gaaactactt actcatgctc acttggtttg agaactcacg caaaaaaact ttctacaatg
     4441 caatttgcag gaaaaatctt tgttttgtca tcccaactga tctatgctga tgcatagatt
     4501 taacttaaag gcatagacag attgaattta gtggttttta taaccaatgc tcaggaatag
     4561 ataaatcaaa tgatctggat acgtatcgaa gagtaataat tatctcctca tatctctttt
     4621 gatcgatagg tttcccctcc tttcgcaaca gtaatatttc ttgcaattct cttagattaa
     4681 acaaatctag cttataaaaa tttccccacg ttggttggcc gaaaaggtta taaattgtga
     4741 ctgtgccatc cctctcacta gcgagtcttt tctctccaga aatttttgac aaagccactt
     4801 cggcttcatc tttatcttca taaatgttaa gcagcttcac tgttaacccc caacctcacc
     4861 tatataaaat agaaatcaaa accattactt ctgatagttc agtttaccaa aatcgaacgg
     4921 acttaccgct ttctcctgat tggcatccag aaaatctgtc caccactgta gcatcagttg
     4981 acgttcttct agatgttctg ctttatggat atacgcagca cgtacaccat tacgctcctg
     5041 atggctcatc tgaagttcca cagcatcgtg ggaccacaaa ccttattcaa tcaatgcact
     5101 gcgcgccatc gcccggaatc catggccaca gattacctgg gttgtgtcat attccataac
     5161 cctcagggct ttatttacgg tattttcact gattgggttg cgcgggtcat gtgctcccgt
     5221 aaagatcaga ccattttcac cagctcatgt cttaagttct gtcagtatgg ctacagcctg
     5281 tttgctgagt ggcacaaggt gcggtgttcg aatcctggtt ccccaatgag agttcaggcg
     5341 gtgcgagcaa ataaaaaaag aaaacataaa taaaaccatc cagctgttag ggctggatgg
     5401 ttattcatta ttttagtgtc actgagcttt cattcatcag gcctgctcag aaagtgaatc
     5461 aatgtagtca gcataccatt gcatcatttc acggcgaccg tcgagatact gggcatgatt
     5521 ataggtaccc ctgatggagt ttttatccac atgagctaac tgcatttcaa tccaggcaga
     5581 attaaagccc tgttcgtgca gaatggtact catggtgtga cgaaaaccat gaccggttgc
     5641 cctgccatcg taaccgatac gcttgagcac catgttgatg ctggcttcgc tcattggctt
     5701 gttgatatca caacgtcccg gaaaaaccag ccggtattgt ccggagaaag ttttgagctg
     5761 cagcagaatg tcagtggtct gtcgtgacag tggtacgaca tgtttacgtc gcattttcat
     5821 gcggctttca gggattgtcc acagggcatt atcaaaatca atttctgacc attcagcgga
     5881 ccgtagttca attgttctga ggcctgtcag aagcagcaga tttgtcgcca tgcgggtaac
     5941 aggactgcca tgatattcag cgagcttccg gagaaaaaca gggagctcat ctgctctcag
     6001 gtgggggaaa tgcgccgctt ttggcgccgc cagcgtactg gtcagttcag atgccggatt
     6061 gacagtggct cgtccggtgg cgatggcata acgaaaaatt tgattacagt attggcgcac
     6121 cttacgcagt ttttccagcg agccacgttt ttctatgcgg cgcagaattt ccagcatttc
     6181 cagcgggagg atatcagtaa cgcttcgttt gcccagatac gggaaaatat ctttatccag
     6241 agcctccagt acgcttctgg cgtaactttc agaccatgac aaagatttat gctggtgcca
     6301 ttccgtagca agggcatgga gcgtattggt ggcagagatt ttctgagtga gtttttcctt
     6361 tcgcttcttt tcgcccgggt caccaccttc ggagataatt tttcgggcat cgtcgcgttt
     6421 gattctggct tcagcaaggg agacgtcggg atagacgccg agggccagtt ttttctcttt
     6481 tcctgctatc cggtatttaa gccgccagta gcgggcaccc gtggttttta ccagcagata
     6541 taaaccacgt tcgtcagaga gtttatagtc tttttcttta ggtctggctg tttcgacctg
     6601 tctggctgta agtgccatat gggggctcct gaaactgact gaacacaatg agcccccacc
     6661 ggtgccccca atgaacgctt gattgagaga gagttcagta gagttcggtg gagttcagga
     6721 tgttcagatt gtttgtaacg atgtgattat acttgagttt cgtggagttc gggagatgaa
     6781 tctggaagtg aaagtggtgc ccggactcgg aatcgaacca aggacacggg gattttcaat
     6841 cccctgctct accgactgag ctatccgggc aacggggcgc attaaacctg attcgcctcg
     6901 tctcgtcaat caaatttctt caatttactg cagactgcac aatctatcat cactttgccg
     6961 ctattgcacg cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg gcattgccag
     7021 aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga tgcgtaatcc
     7081 ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg acggtgtggt
     7141 cagcggtaat atcagcgcgg cagcccacgc cagcgttaac tggatgatca gtaatgggat
     7201 actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg taaagaaatc
     7261 aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt tcatcgcccc
     7321 gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat tctgaataaa
     7381 agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt tggcgactgc
     7441 tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc
     7501 taaaaccatc gctggaaggt tggcgcattt cacaaccagt aaaataccaa tccccagcga
     7561 tgccagacca aaaatagatg tcatggagac tcggatacgt tccggtaagc gttggctgag
     7621 cagtgcgccg agaacgccac ccagtaagac tgcactagcg ttgataaaag ggccgatgac
     7681 cacaagagtt cctgttagct gtattgtttg attttcttat tatgaacttt ttgccttgtt
     7741 gatggcttgt ctggtcgcaa agtcctggct tgcacgcttt agcgaaaggt gccatgattg
     7801 cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccatctcc ttacattctc
     7861 tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat gttactgctc atgcggtgaa
     7921 gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct tttactgatg
     7981 tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg gttttccacc
     8041 ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc
     8101 cgccagtagt gaactggtat cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga
     8161 tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc gcagtggcat
     8221 tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt ccgaacatgc
     8281 tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc agtggctgga
     8341 gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt atgacacgct
     8401 gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc aacatggtgc
     8461 gttttttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg agaacgtctt
     8521 tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc atgaaggatc
     8581 ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa cctattttgt
     8641 gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga
     8701 tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc tgattcaggc
     8761 gggggcgacg ccggtctatc tggaagcttc acgcaacccg tttggtttca ttggcggtat
     8821 tgatgcgcac tgttttaatg aagagtatct gcgccagcaa attcgcgacg ttgcgccaga
     8881 aaaagccgag ctgccgcgcc cgtttcgcct ggcgattatt cagctgggaa cctatgacgg
     8941 aactgtctat aacgcccgtc aggtgatcga taccgttggg catctgtgtg attacattct
     9001 gtttgattcc gcgtgggtcg gttacgaaca atttatcccg atgatggcgg atagctcgcc
     9061 gctgctgtta gaacttaacg aaaacgatcc ggggattttt gtgacccagt cggtgcacaa
     9121 acagcaggct ggattctcac agacgtcgca gatccataaa aaagataacc atatccgcgg
     9181 acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc tttatgctcc atgcttctac
     9241 cagccctttc tatccgctgt ttgctgcact ggatgttaac gccaaaattc atgaagggga
     9301 gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat
     9361 tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg
     9421 gcaggattat ccgacatctg tgttagccag cgaccgccgt tttttcagtt ttgagccggg
     9481 ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg atccgtgcaa
     9541 gctgttactc actacaccag gtatcaatgc tgaaactggc gaatatagcg actttggcgt
     9601 tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg agaagtgcga
     9661 tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc tggcacaact
     9721 ggtggcgatg ctggcgcaat ttgaacagca tattgaggat gactcgccgc tggctgaggt
     9781 gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacaccc tgcgccagtt
     9841 gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat
     9901 gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc atagcgctta
     9961 tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga
    10021 aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag tctggggtgg
    10081 ggcggttcaa cgttatttcc ttgcactgga agaaggggtg aatttgttgc cgggattttc
    10141 gccggagctg caaggtgttt atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg
    10201 ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg
    10261 cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac
    10321 atcgccatga acgcgaaggc cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc
    10381 tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct cacgatgccg
    10441 ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc actggcgcga
    10501 attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa gaagtcgaat
    10561 gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg agtcgggtcg
    10621 ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat cattacgttc
    10681 ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt ttcagagatc
    10741 tgcgaaatcg acatgatgat tgacgcatgc tgcacaataa agctgctggc aaacatcgga
    10801 tctttgtcga agctgtgcag gaaggtatta ccgaacatat tggtaatctg cagttccgcg
    10861 cccagcagca ttgagaagat aaagaagatt gccatacgct tgtttttaaa cagcgcgaat
    10921 gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc aaccggaata
    10981 tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc aatatacagc
    11041 tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat gataaagccg
    11101 atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc ggcattttgc
    11161 aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc aaacgagtta
    11221 atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat gaacagcgtg
    11281 atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca tttgtcggcc
    11341 acaatcccca gcagcgcagg cataaagacc gcggcgatac ccagtgagct ataaactgcg
    11401 ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag ggtcgtcagc
    11461 caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag cttaagattc
    11521 atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc
    11581 gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg gcaaataatt
    11641 gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt gcccctgagc
    11701 atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc ggtattcact
    11761 ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca
    11821 tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt cgaaaagact
    11881 cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg ccgcgatgtt
    11941 gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc ggtgccggta
    12001 tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc
    12061 gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg
    12121 gaacggctga ccgcatatgg gttaaaggaa gattcggtct gcataatcgc cagaatcagc
    12181 gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata tttgtgcgca
    12241 cgtttatcaa ggtggttcgg caccatgtta atggtaacgc tgtagatgat acgcagcccg
    12301 ttgctgcggc tcttcagacg gtttttcagc agataatccg cgaagttgct cgcgcgacct
    12361 tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa aggttctttc
    12421 gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc gtcacccatc
    12481 agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc ggcgatagtt
    12541 tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata ttgatcggtg
    12601 tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca gatgttctca
    12661 atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt gacccaggct
    12721 tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa
    12781 gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagt
    12841 tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat agccctcgat
    12901 atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca gcttgcggtg
    12961 ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt tgtgctgcca
    13021 ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct cgccggggta
    13081 cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct
    13141 cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg
    13201 taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta agttatacca
    13261 gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc acataggcac
    13321 aatatctaag tggaaatggc tgaaggtatg ccgaaacgcg gtcagctgcg tcaggttatc
    13381 ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt tcttcgtcgg caaactgcgg
    13441 gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca atacttcatc
    13501 ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt tcggtttttt
    13561 gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt gtagcggaca
    13621 gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca tcatcgcctg
    13681 attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc ataatttatt
    13741 ctcgacctct tttttccctg gccagccgct tacagcatag cagcgcgcca gcacccgttt
    13801 gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa tcgcacctgc
    13861 ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg ggaatttacc
    13921 gccgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc gggcgtaata
    13981 gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga gatcggtcac
    14041 cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa cctgagtttg
    14101 ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt gccagggcag
    14161 cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg
    14221 catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac ggcagggtgt
    14281 aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac taactttgga
    14341 taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa acgacgtcat
    14401 ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt ttgtgcgccg
    14461 ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc cggtgatggg
    14521 cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg aagcgccggt
    14581 gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta aagatcgccc
    14641 tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc
    14701 tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct
    14761 gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc ctgacccgtg
    14821 gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg aactggtaaa
    14881 aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac cttatgcgga
    14941 acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag agagcaatga
    15001 ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct
    15061 tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa gaaccgtagc
    15121 cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt ttcggtggca
    15181 tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga tgattttatt
    15241 aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct ggcctgggaa
    15301 ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt
    15361 aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt cgacgtttgg
    15421 tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta
    15481 tcaggcctag aaagatgcac gatcgagtag gcgggataag gtgtttacgc cgcatccggc
    15541 atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct gattttttct
    15601 tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt gacggcaatg
    15661 accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg
    15721 caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga cggcaacgtg
    15781 atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc gaaggattat
    15841 caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgaa
    15901 aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag cagcaaaatg
    15961 cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa
    16021 acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg cgccgaaggc
    16081 cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa tgaaatgggc
    16141 gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg aagcctgggg
    16201 gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt
    16261 ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc cctggtcggg
    16321 aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg tttaattctt
    16381 cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt
    16441 ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac gtaactggag
    16501 gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg gttttagtgg
    16561 tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca ttgccggtgg
    16621 tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag aaaatctggt
    16681 taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg tagtgaatat
    16741 cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga
    16801 ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg aagaaaccgc
    16861 ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg
    16921 cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg tagtggccgc
    16981 agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca ccgtactgga
    17041 cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt ctgttaacta
    17101 cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc cggcacgtaa
    17161 gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga aagtcggcat
    17221 cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag atgcgggcta
    17281 tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg tgttcgacac
    17341 cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg taccgacggg
    17401 tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg cctctggcac
    17461 gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa ccaaagatcc
    17521 gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta tcgtgccggg
    17581 gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg ataatcaccg
    17641 gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt tggcacggca
    17701 ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac tgaaaatatc
    17761 ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc tatcacctta
    17821 aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga tgtctcttcc
    17881 agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct cgaacagacc
    17941 tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg
    18001 tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg tgggcgggtg
    18061 gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga tttcaattat
    18121 cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc tgcgctggaa
    18181 aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc ggatgcatat
    18241 tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa aaaaattcac
    18301 caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt taaatatgac
    18361 aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga gtgttatgaa
    18421 gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca gacttttctg
    18481 caatcacaaa aactcagccg taaattgcag gatgactggg taacgcttga aaaagggcaa
    18541 tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat tttgatgaaa
    18601 attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca actgcggcgc
    18661 aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa aaagttatcc
    18721 ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat atttgcggaa
    18781 tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc aatggtttga
    18841 ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca ctacccaccg
    18901 gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa tgtactgggt
    18961 ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt acagccggtg
    19021 tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc aatatgatca
    19081 gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata tcagcattag
    19141 tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc gaagtggtaa
    19201 caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg taaaccagca
    19261 ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg gtaaaaatgc
    19321 cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag gcatccagaa
    19381 acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca cggcgtttag
    19441 caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc aaatatcccg
    19501 ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca attaacacgt
    19561 ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg gtagaaaatg
    19621 ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg gcagcagctg
    19681 ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg ttcacatgac
    19741 caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa cgtatcgcaa
    19801 taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc aatagggcaa
    19861 agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata tcccagggaa
    19921 acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg ggcacaccaa
    19981 taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta tccattaact
    20041 ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac acaaggcata
    20101 aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca tcggtatttg
    20161 catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca ggccaccagt
    20221 cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac taagaagtaa
    20281 ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc tctgcgcgcc
    20341 gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg ccagctcatc
    20401 ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga ctacgcatac
    20461 aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta ccgcaagttt
    20521 caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac tggcccggaa
    20581 tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg atcttttttg
    20641 aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac ttttacaatt
    20701 tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc ccacaatttt
    20761 tgttgataag cttcggattt cttcgctgct gtttcgagga tttgttgctg ttctggggtt
    20821 aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc gtgttcatct
    20881 tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg cacgttattt
    20941 tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa tgaaattgga
    21001 gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg ctgaacacga
    21061 attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata gaaactgcgt
    21121 gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga gtccataatt
    21181 tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt aaataagaac
    21241 ggcaaattat agatagcata aatattatca aaagactcca gatcactggc ggaacctttg
    21301 gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt acccatctgg
    21361 ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac ctctttcgcc
    21421 agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc taactttaac
    21481 gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag ccagcaagga tgcgcagact
    21541 aatgagcggg taaatgacct cattttcata aaaaaactct ctgttgaagg atggaaattg
    21601 gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac ctgtctgaca
    21661 acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta aatttgtgcg
    21721 cca
//
LOCUS       DAZFDP010000074        21690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFDP010000074 DAZFDP010000000
VERSION     DAZFDP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            158..886
                     /locus_tag="ACHJ1G_002836"
     CDS             158..886
                     /locus_tag="ACHJ1G_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4764664.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            886..5736
                     /gene="ehaG"
                     /locus_tag="ACHJ1G_002837"
     CDS             886..5736
                     /gene="ehaG"
                     /locus_tag="ACHJ1G_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4764665.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6105..7760
                     /gene="lldP"
                     /locus_tag="ACHJ1G_002838"
     CDS             6105..7760
                     /gene="lldP"
                     /locus_tag="ACHJ1G_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4764666.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7760..8536
                     /gene="lldR"
                     /locus_tag="ACHJ1G_002839"
     CDS             7760..8536
                     /gene="lldR"
                     /locus_tag="ACHJ1G_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4764667.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8533..9723
                     /gene="lldD"
                     /locus_tag="ACHJ1G_002840"
     CDS             8533..9723
                     /gene="lldD"
                     /locus_tag="ACHJ1G_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4764668.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9909..10382
                     /gene="trmL"
                     /locus_tag="ACHJ1G_002841"
     CDS             9909..10382
                     /gene="trmL"
                     /locus_tag="ACHJ1G_002841"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4764669.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10435..11256)
                     /gene="cysE"
                     /locus_tag="ACHJ1G_002842"
     CDS             complement(10435..11256)
                     /gene="cysE"
                     /locus_tag="ACHJ1G_002842"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4764670.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11336..12355)
                     /gene="gpsA"
                     /locus_tag="ACHJ1G_002843"
     CDS             complement(11336..12355)
                     /gene="gpsA"
                     /locus_tag="ACHJ1G_002843"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4764671.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12355..12822)
                     /gene="secB"
                     /locus_tag="ACHJ1G_002844"
     CDS             complement(12355..12822)
                     /gene="secB"
                     /locus_tag="ACHJ1G_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4764672.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12885..13136)
                     /gene="grxC"
                     /locus_tag="ACHJ1G_002845"
     CDS             complement(12885..13136)
                     /gene="grxC"
                     /locus_tag="ACHJ1G_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4764673.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13278..13709)
                     /gene="yibN"
                     /locus_tag="ACHJ1G_002846"
     CDS             complement(13278..13709)
                     /gene="yibN"
                     /locus_tag="ACHJ1G_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4764674.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13954..15498
                     /gene="gpmM"
                     /locus_tag="ACHJ1G_002847"
                     /gene_synonym="pgmI"
     CDS             13954..15498
                     /gene="gpmM"
                     /locus_tag="ACHJ1G_002847"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4764675.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15532..16791
                     /gene="envC"
                     /locus_tag="ACHJ1G_002848"
     CDS             15532..16791
                     /gene="envC"
                     /locus_tag="ACHJ1G_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4764676.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16795..17754
                     /gene="yibQ"
                     /locus_tag="ACHJ1G_002849"
     CDS             16795..17754
                     /gene="yibQ"
                     /locus_tag="ACHJ1G_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4764677.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17741..18775)
                     /gene="waaH"
                     /locus_tag="ACHJ1G_002850"
     CDS             complement(17741..18775)
                     /gene="waaH"
                     /locus_tag="ACHJ1G_002850"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO4764678.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19026..20051)
                     /gene="tdh"
                     /locus_tag="ACHJ1G_002851"
     CDS             complement(19026..20051)
                     /gene="tdh"
                     /locus_tag="ACHJ1G_002851"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4764679.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20061..21257)
                     /gene="kbl"
                     /locus_tag="ACHJ1G_002852"
     CDS             complement(20061..21257)
                     /gene="kbl"
                     /locus_tag="ACHJ1G_002852"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4764680.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21532..>21690)
                     /locus_tag="ACHJ1G_002853"
     CDS             complement(21532..>21690)
                     /locus_tag="ACHJ1G_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO4764681.1"
                     /translation="DQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQA
                     LRIFLSRK"
BASE COUNT         5498 a         5637 c         5535 g         5020 t
ORIGIN      
        1 ttttgaaacg aatgatacat ttttatgatg aatgcttaaa acaatattta atcgacacac
       61 atcactgtca ttaatgtaaa gcaacataga tcaccaataa tacaatggac aactctaccg
      121 gcattgatcg ctggtcggag gaaataagga tttttacatg aacttgaaga agattttttt
      181 cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca ttgaagaagg
      241 caagccagaa agtagtttat tgaaacaaca agaagaacat aacaataaaa ttgatctact
      301 tgaaaaacaa caagcacagc taaagagtca actggaaact attcaaaaac aacaaactgg
      361 cataataagc agtacaaaga ctttaactca cgtaattaaa tccgttaaag atcaacaaaa
      421 cacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc tgaacaatgg
      481 ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg gcagtgttat
      541 tcatatttca ctggtcaact tattaagtac acctatctca aatattggtt ttaatgcgac
      601 atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac aattactttt
      661 caacacatca atgaaatcca cattgaaatt attaccaggt caatggcaag acattaattt
      721 gaccctgaag ggtgtatcgc ccaataacct gggatatctg aagttagcca tcaacatgga
      781 aaatattcag ttcgacaatc ttccttctgc tgaaaatcgg cagaaaagaa gcaaaaaaat
      841 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
      901 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
      961 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
     1021 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
     1081 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
     1141 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
     1201 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
     1261 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
     1321 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
     1381 atgggggact cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa
     1441 gctactgaga ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca
     1501 atggcgctgg gagcaagtag cgtcgcatct gaagaaaacg caattgccct ggggcgtagc
     1561 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
     1621 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
     1681 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
     1741 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
     1801 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
     1861 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
     1921 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
     1981 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
     2041 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     2101 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     2161 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     2221 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     2281 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     2341 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     2401 gatgtcgaag ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaatacg
     2461 gcaaatattg ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt
     2521 caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag
     2581 gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat
     2641 gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat
     2701 atcgccaata acacttccaa tattgccact aacaccacca acatctctaa cctgactgag
     2761 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg
     2821 gcagctcatg gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg
     2881 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
     2941 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
     3001 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
     3061 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
     3121 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
     3181 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
     3241 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
     3301 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
     3361 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
     3421 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
     3481 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
     3541 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac
     3601 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
     3661 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
     3721 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
     3781 ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
     3841 aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccttgctg
     3901 tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
     3961 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
     4021 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
     4081 ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat taactatgtg
     4141 cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt tggcgctaca
     4201 gctataggtt ataactctgt cgccaaaggc gatagcagcg tagctattgg tcagggcagc
     4261 tacagcgacg ttgatacggg tatcgccctg ggtagcagct ctgtttccag ccgagtgatt
     4321 gccaaaggct cccgtgacac cagcataacg gaaaatggcg ttgttattgg ttacgacacc
     4381 acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata tcgtcaaatc
     4441 atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca attgcagaat
     4501 gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc aacggaagaa
     4561 gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc
     4621 gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact taacggcatt
     4681 gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg taatggttct
     4741 acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc accgcagaac
     4801 tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac taacgtcgca
     4861 gcaggttcgg ctgataccga tgcggtcaac gtgggtcagt tgaaagtaac ggatgcgcag
     4921 gtttcccaga atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact
     4981 cgcgttacta atatcgaaaa cggtattggc gacattgtca cctccggtag caccaagtac
     5041 ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt
     5101 ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca
     5161 gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta
     5221 gctgccggtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca
     5281 ggcggcgtgc gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
     5341 ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
     5401 gacgcggtga actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat
     5461 cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
     5521 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
     5581 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
     5641 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
     5701 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
     5761 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
     5821 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
     5881 ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc
     5941 aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt
     6001 atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc
     6061 aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac
     6121 aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc
     6181 tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga
     6241 cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc
     6301 tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg
     6361 cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt
     6421 ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg
     6481 gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc
     6541 tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg
     6601 cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca
     6661 tcgacagttt tgagattggc cagatggtgg ggcgtcagct accgtttatg accattatcg
     6721 tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaagag acgtggcctg
     6781 cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctcc aacttcattg
     6841 ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc
     6901 tcaaacgctg gcaaccagtt cgcgtattcc gcttcggtga cttgggggcg tcacaggttg
     6961 atatgacgct ggcccacacc ggttacaccg cgggtcaggt gttacgtgcc tggacaccgt
     7021 tcctgttcct gaccgctacc gtaacactgt ggagcatccc gccgtttaaa gccctgttcg
     7081 catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac
     7141 tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt
     7201 ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct
     7261 ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg
     7321 ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg
     7381 gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct
     7441 cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc
     7501 tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg
     7561 ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta
     7621 tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca
     7681 aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct
     7741 taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     7801 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     7861 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctacgcgagg cgctagcaaa
     7921 actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     7981 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga
     8041 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     8101 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     8161 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     8221 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     8281 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     8341 actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc
     8401 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     8461 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag
     8521 ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt
     8581 ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc
     8641 cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtcc
     8701 gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca
     8761 ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg
     8821 gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc
     8881 gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt
     8941 atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg
     9001 gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat
     9061 gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc
     9121 ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga
     9181 ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac
     9241 cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg
     9301 gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt
     9361 ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg
     9421 gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg
     9481 cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg
     9541 gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa
     9601 gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg
     9661 caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca
     9721 tagtcgtttg cgccccctca ccctaaccct ctccctcagg gagaggggac cgttcggcgc
     9781 tgtatgtact ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg
     9841 gggtgagggg aaaaggccgc actgctcacc aattttctgc tatcctgccc ccgcactaag
     9901 ggggcagtat gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaata
     9961 tcatccgtct ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg
    10021 cctgggacga taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta
    10081 cgcgtcatca tgactttcgc gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc
    10141 tcactacgaa aggtacgcct gctcacagcg ccgtaaacta tcaggatggc gattatctga
    10201 tgttcggccc ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac
    10261 aaaaaattcg cattccaatg gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt
    10321 cggtagtggt gtatgaagcc tggcggcagc tgggatatcc gggagcgttg ttgagagatt
    10381 agttactgta tgccggatag atgattacat cgcatccggc acgatcacag cacatcagat
    10441 cccatcccca tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga
    10501 tggcttatcg ctgtctggtt taccgacaat acgagccgga acaccagcgg cggtagtatg
    10561 cggcggcacc ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc
    10621 aatattgccg agaattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg
    10681 acggtcacca ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc
    10741 gttttcaatt accgccgttt caccaacgac gatgcccgtg gcgtggtcga gcatgatacc
    10801 gcgaccaatt tttgctgccg ggtgaatatc gacctggaac gtcacagaaa cctggttttg
    10861 cagaaagatt gccagtgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc
    10921 ctgcaaggca tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg
    10981 gtcgcgggta cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc
    11041 ggcgtaggct tcttcaacca cttcacggat agcaatagca ggcataattg gcgatgacag
    11101 cttgttcgcc agcatgtagc tcagggcact gccaaggttt tcgtgcttga gtagcgtcgc
    11161 gtggtaaaaa ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat
    11221 attgttccag acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa
    11281 tgaccggccc gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt
    11341 ggctgctgcg ctcgtccttg cgtgcacgac ctagtaaagt caatgctgcc tcgcgcgcgt
    11401 tttttccgca atataatact tgataaattt cctcggttat tggcatttca acgccgaagc
    11461 gatgcgccag ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct
    11521 tctcctgcgc gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac
    11581 gcgactggtt gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg
    11641 tggcaggatc ggcacccagc gccgcaccaa gacgcgacat ttcagccagc ccacgggtga
    11701 tcagcgccgt acgcgcattc gcaccaaaac cgataccgtc agacatcccc gcgccaatgg
    11761 caatgacgtt tttcaccgcg ccgccaagct gcacgccaat gaaatccggg ttgctgtaaa
    11821 cgcggaaact tttgccacag tgcagcaatt gttggagatc atcggcaaag gtctggtcgg
    11881 tagaggccaa cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc
    11941 cagagataac cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca
    12001 gacgtccggt ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca
    12061 tcagcggttt aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa
    12121 tattacggct ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa
    12181 aaggcacatc ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt
    12241 cagggtcatg gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag
    12301 cggtgccgta cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc
    12361 atcctgatgt tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc
    12421 atcgaagtta accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt
    12481 gatgcactca cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc
    12541 catctgggtg ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag
    12601 gaacgcggtt tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtatac
    12661 gtcatctgcc agttgggaag atgccgtatc cagatcaagt ttaacttctg gttgccaatc
    12721 tttctggaaa acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg tataaatacg
    12781 ttggatctgg aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa aacccttaag
    12841 tgttgtcctt aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga
    12901 tccagtccac cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg
    12961 tcaataaaaa tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg
    13021 gcggcattgc catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct
    13081 tttgcacgat ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataatt
    13141 tagctcctgt ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc
    13201 cccagaagct tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat
    13261 gaaagacgac aggtaaatta tttgccgcgc accagaggca agttttcgcc agcccagcca
    13321 gcaacgccct ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca
    13381 ggctcctggc actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc
    13441 tcaagctcac caacattgtt ggctttgatt tcactcggca acagattgat agaacctgcg
    13501 atatggcctt tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg
    13561 ttgatcagac gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta
    13621 aacgtagtca caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga
    13681 cggccaacaa attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa
    13741 aaaaaccagg aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg
    13801 aatgcaattt tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga
    13861 gcggtcgtat attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg
    13921 caaattttga ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta
    13981 ctggtgattc tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt
    14041 gctaaaaccc cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct
    14101 tccggtctgg aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt
    14161 aacctgggtg ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa
    14221 gatcgcgctt tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca
    14281 ggtaaagcag tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat
    14341 cacatcatgg cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac
    14401 gcattccttg acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt
    14461 gaagagaaat ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac
    14521 gcgatggacc gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg
    14581 gcgcagggtg agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc
    14641 gacgaaaacg acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg
    14701 gcaatggaag acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa
    14761 atcactcgtg cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa agtggttaac
    14821 gtcgatttcg tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca
    14881 cccgcttccc tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg
    14941 cgtatttccg aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa
    15001 gagtcgttca aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat
    15061 ctgcaaccgg aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc
    15121 ggcaaatacg acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg
    15181 gtgatggaag cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg
    15241 aaagcggttg agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa
    15301 cagatgcgcg atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg
    15361 ctgatttatg ttggtgataa gaacgtgaaa gcggttgaag gcggcaaact ttctgacatc
    15421 gcgccgacca tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg
    15481 ctgttcatcg tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg
    15541 gccgtgaaac cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct
    15601 ggcgtattgt tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt
    15661 caggccgata tcgccgcaaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc
    15721 ctgctcgcac aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc
    15781 gaaacgcaaa acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt
    15841 gccaaactgg agcagcaaaa agccgcccag gagcgcagcc tcgccgcgca actggatgcc
    15901 gcattccgtc agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag
    15961 cgtggacagc gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt
    16021 gcccagttga agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa
    16081 caaagcgagc aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag
    16141 gctctgaacg agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag
    16201 cagcagctaa gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg
    16261 gaagccgcgg cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac
    16321 cgccagaaag aagcgacgcg caaaggcacc acctataaac cgaccgaaag cgaaaaatcg
    16381 ctgatgtccc gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc
    16441 gggccgacgc tgcatcgcta tggtgaacag ctacagggcg aactacgctg gaaaggaatg
    16501 gttatcggtg cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg
    16561 gctgactggc tgcaaggtta cggtctggtg gtggtggttg agcacggtaa aggcgacatg
    16621 agtctttacg gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc
    16681 cagccaattg cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa
    16741 attcgccgcc agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt
    16801 ccatttcgtc gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt
    16861 gctggcaaac ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag
    16921 gtgctggcga tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga
    16981 gagatggcga ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggca
    17041 ccactgagta aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa
    17101 attgagcgca ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac
    17161 cacatgggta gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcgggcactg
    17221 gagcgttaca atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc
    17281 gccgcgcaag gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag
    17341 aacgaagcgg acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt
    17401 tcaaccattg ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt
    17461 tataacctgc cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag
    17521 gttgatactt ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt
    17581 ggcgtgaagc tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt
    17641 gaagtattaa gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg
    17701 caaggctggg gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc
    17761 tacgcgtaat gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc
    17821 acagtaacgc ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca
    17881 taccggaagt aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc
    17941 gcaccgcatg gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa
    18001 tcgtaatttt gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat
    18061 agtgacgttg ataattaagg tttttattcc cttgtctatg caaccgactc actgacgtat
    18121 tatgcagata atatttatat aatgattgct cggtatatcg cgcacgcagc gcgttaaaca
    18181 tgaattctgt tgtccagaca atatcctgat gatgtaatcc ggcaataaat ttaatgttat
    18241 ttttaacaat aacatcacgg cgataaaccc ccatccagac aacgtgagtc caacggcgcg
    18301 aagaaagccc catccgcagc cagtccgggc cggttaatac gccggttgag cgaaggcgat
    18361 cgctggggat ggattgccag gtttctcccg tttcacgaaa acaccagtca gcgttgcact
    18421 gcgccacgtc gaggtcatcc tctaacgcca tggtcatcag cgtttcgtac atggtgggat
    18481 agacctcatc gtcagcatcg acaaaagcga catatttgcc cgtcgccacc tcgatcccac
    18541 gattacgcgc taccgatgcg ccagcattcg cctgatgcaa caaacgaacg tgcggatagt
    18601 tttctgcgta atgctttgct atttcaacag aattatccgt tgaaccatcg ttaataataa
    18661 tgatttccag agcagtccag gtttgcgtaa ttaaagattc catacaagtg cggaaatcat
    18721 cgcccgcatt atataacgga ataataacac taagtttatt ggtgctgttc atcataaatt
    18781 accgacgacc tctgaagtta tcctgacgtt ttacaggctt tgtattaaga aactattaag
    18841 cctgtgagat attattttta caattatttt tacaggtgag agagtcactt cagggtagag
    18901 agataatgag agcagctata taagagatca gcgcaattac gatatcttta ttacactgac
    18961 aggattttac atatttaacg gattatcctc aggcaaaagg gctggaatac cagcccttgt
    19021 tcgtgttaat cccaactcag aattactttc ccggactggc ccgaacgcat agcgtcaaag
    19081 cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag atccagacct
    19141 gactgaatca gcgccgccat cttgtaccag gtttcaaaca tctcacgacc gtaaatacct
    19201 ttaatgaaca agcctttaaa gattactttg gtccagtcga tagacatatc ggacggtgga
    19261 atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt acgaaacgct
    19321 ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc cagttcagcc
    19381 atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc cattttgcgc
    19441 gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc accaacgtgt
    19501 ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag cacatcttcg
    19561 cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc aaaaatggaa
    19621 gccaggtcgt cggagatatt gtctgggatt ttgaaggcgt tgaacgccgg gatcaccaga
    19681 tattcggcga agcagcccgg acggttaacg ccgacgccga tcgtgttgcg gcacagatgg
    19741 gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc gccagaaacg
    19801 cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc accgacatat
    19861 tcatggccta cgaccatcgg caccgggatg gttttttgcg accactcatc ccagttatag
    19921 atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag atcgttatgc
    19981 ccgagttccg gtacaggaac gtcggtcatc cagatgccct cttccgcttt cagtttggat
    20041 aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat acgcgtgaat
    20101 gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg ggtacgaata
    20161 cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtaaat gccctctttt
    20221 tgcagctcac gggcaaattt ctgcgccact actgcatcgc caagcatgac cggaataatg
    20281 gcatgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg gaactggcgc
    20341 gcgttcgccc acagacggtc acgcagttcg ctgcccgctt cgaccatctc cagtactttg
    20401 atggacgcgg caacaatggc cggtgccagc gagttggaga acaggtacgg acgagaacgc
    20461 tggcgcagcc actcaaccac ctctttgcgc gccgcggtat aaccaccaga agccccgccc
    20521 agcgctttac caagcgtacc ggtgataata tcgacccggc ccatcacatc gcagtattca
    20581 tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc taccatcacc
    20641 agggcatcgt atttatctgc cagatcgcaa acgcccttca gattggcaat cacgccgtcc
    20701 atcgagaata caccatcggt ggcaatcagc acatgacgcg caccggcttc acgcgcttct
    20761 ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg tttagcttta
    20821 cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat aatggcgtct
    20881 tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga agagtagaga
    20941 atcgcatctt ccatccccag gaaggccgcc agtttttgtt caagctcttt atggctgtcc
    21001 tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc cattcccgcc
    21061 tttgccgccg caatcagatc aggatgattc gccagcccga gatagttgtt ggcacaaaag
    21121 ttaatgacgt ggcttccatc agccacagtg atatctgctt gctgcgcaga cgtaataatg
    21181 cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt taactgctga
    21241 taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca tattacccaa
    21301 acttataggt cgcgacgaga tgacgcgtta acacttctgc aaaattcagg ataaataacg
    21361 tgtaacttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt tttggctggg
    21421 cataatgatg attgatttta ctgcattgat ataaaaagaa aaaatgatgc caaagcatca
    21481 tttttcctat ctcagttggt atcaaaacgc tacccactaa tggagggaaa cctattttct
    21541 tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc tgaagcagcg
    21601 aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat cggggcgttt
    21661 gtaatagcac atgacaaaaa tgccctggtc
//
LOCUS       DAZFDP010000075        21296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFDP010000075 DAZFDP010000000
VERSION     DAZFDP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..239
                     /locus_tag="ACHJ1G_002854"
     CDS             <1..239
                     /locus_tag="ACHJ1G_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4764682.1"
                     /translation="QCPITLAIPQRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPL
                     SREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            410..645
                     /locus_tag="ACHJ1G_002855"
                     /pseudo
     CDS             410..645
                     /locus_tag="ACHJ1G_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1055..1156)
                     /locus_tag="ACHJ1G_002856"
                     /pseudo
     CDS             complement(1055..1156)
                     /locus_tag="ACHJ1G_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1380..1505
                     /locus_tag="ACHJ1G_002857"
                     /pseudo
     CDS             1380..1505
                     /locus_tag="ACHJ1G_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1565..2071)
                     /gene="yciE"
                     /locus_tag="ACHJ1G_002858"
     CDS             complement(1565..2071)
                     /gene="yciE"
                     /locus_tag="ACHJ1G_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4764683.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2117..2617)
                     /gene="yciF"
                     /locus_tag="ACHJ1G_002859"
     CDS             complement(2117..2617)
                     /gene="yciF"
                     /locus_tag="ACHJ1G_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4764684.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2703..2882)
                     /gene="yciG"
                     /locus_tag="ACHJ1G_002860"
     CDS             complement(2703..2882)
                     /gene="yciG"
                     /locus_tag="ACHJ1G_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4764685.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(3263..4069)
                     /gene="trpA"
                     /locus_tag="ACHJ1G_002861"
     CDS             complement(3263..4069)
                     /gene="trpA"
                     /locus_tag="ACHJ1G_002861"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4764686.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(4069..5262)
                     /gene="trpB"
                     /locus_tag="ACHJ1G_002862"
     CDS             complement(4069..5262)
                     /gene="trpB"
                     /locus_tag="ACHJ1G_002862"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4764687.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(5274..6635)
                     /gene="trpCF"
                     /locus_tag="ACHJ1G_002863"
     CDS             complement(5274..6635)
                     /gene="trpCF"
                     /locus_tag="ACHJ1G_002863"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4764688.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6636..8231)
                     /gene="trpD"
                     /locus_tag="ACHJ1G_002864"
     CDS             complement(6636..8231)
                     /gene="trpD"
                     /locus_tag="ACHJ1G_002864"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4764689.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(8231..9793)
                     /gene="trpE"
                     /locus_tag="ACHJ1G_002865"
     CDS             complement(8231..9793)
                     /gene="trpE"
                     /locus_tag="ACHJ1G_002865"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4764690.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9815..9908)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9885..9929)
                     /gene="trpL"
                     /locus_tag="ACHJ1G_002866"
     CDS             complement(9885..9929)
                     /gene="trpL"
                     /locus_tag="ACHJ1G_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4764691.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            10067..10948
                     /gene="rnm"
                     /locus_tag="ACHJ1G_002867"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             10067..10948
                     /gene="rnm"
                     /locus_tag="ACHJ1G_002867"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4764692.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10945..11565
                     /gene="yciO"
                     /locus_tag="ACHJ1G_002868"
     CDS             10945..11565
                     /gene="yciO"
                     /locus_tag="ACHJ1G_002868"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4764693.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            11666..12538
                     /gene="rluB"
                     /locus_tag="ACHJ1G_002869"
     CDS             11666..12538
                     /gene="rluB"
                     /locus_tag="ACHJ1G_002869"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4764694.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12578..13168)
                     /gene="cobO"
                     /locus_tag="ACHJ1G_002870"
     CDS             complement(12578..13168)
                     /gene="cobO"
                     /locus_tag="ACHJ1G_002870"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4764695.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(13165..13923)
                     /gene="yciK"
                     /locus_tag="ACHJ1G_002871"
     CDS             complement(13165..13923)
                     /gene="yciK"
                     /locus_tag="ACHJ1G_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4764696.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            14143..15192
                     /gene="sohB"
                     /locus_tag="ACHJ1G_002872"
     CDS             14143..15192
                     /gene="sohB"
                     /locus_tag="ACHJ1G_002872"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4764697.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(15228..15479)
                     /gene="yciN"
                     /locus_tag="ACHJ1G_002873"
     CDS             complement(15228..15479)
                     /gene="yciN"
                     /locus_tag="ACHJ1G_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4764698.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15859..18456
                     /gene="topA"
                     /locus_tag="ACHJ1G_002874"
     CDS             15859..18456
                     /gene="topA"
                     /locus_tag="ACHJ1G_002874"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4764699.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18666..19640
                     /gene="cysB"
                     /locus_tag="ACHJ1G_002875"
     CDS             18666..19640
                     /gene="cysB"
                     /locus_tag="ACHJ1G_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4764700.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            19959..20099
                     /gene="ymiA"
                     /locus_tag="ACHJ1G_002876"
     CDS             19959..20099
                     /gene="ymiA"
                     /locus_tag="ACHJ1G_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4764701.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            20102..20269
                     /gene="yciX"
                     /locus_tag="ACHJ1G_002877"
     CDS             20102..20269
                     /gene="yciX"
                     /locus_tag="ACHJ1G_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4764702.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            20383..20478
                     /gene="ymiC"
                     /locus_tag="ACHJ1G_002878"
     CDS             20383..20478
                     /gene="ymiC"
                     /locus_tag="ACHJ1G_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4764703.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            20642..>21296
                     /locus_tag="ACHJ1G_002879"
     CDS             20642..>21296
                     /locus_tag="ACHJ1G_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO4764704.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT         5320 a         5165 c         5446 g         5365 t
ORIGIN      
        1 tacagtgccc gattacgctg gcaattcctc aacgaggtgt ttttttaaga aatgctgaag
       61 gttccagagt atgtagttta tatgatgaaa tggctctttc tcgtataatt aatgatggga
      121 tgcatcaccc actaagcaga gagccaataa cattatcaat gcttgtggcc agagagcagt
      181 gtgagtttga ttgcagtatc ggtcacttta cggtgaggag tgattgttat tcagtgtaga
      241 ctcggtacac gattttgtgt gaagagactc ttattaaaag ttagcatcaa agcggtcatc
      301 aaacttgata ataaagtgac tcattgctat tcgctggtcc ttaagggata ttgcccattt
      361 tgtatcgtga actgaatagc aagtagccac cgtcctgatt tcaacgcatt atcaggcgct
      421 tctgaagcgg catacctgca tgacaggatg agtgcaaagg ggcgctgtta cgataatgct
      481 agggcggaaa gtttcttcca ctcactaaaa gtcgaatgca tttacggcta atattttatg
      541 agtcgggaaa taatgcagac ggctgtgttt aattagatct agtgtgatta caatcggtgg
      601 tgccgacaca gtacttgtgg ctgtctcagc acggaactgt tttgaaaaac agaaccttga
      661 ttagggctgt gtccacatta cgtaggtagg atcaccccac aggcaccaca tgtcatgcat
      721 ggattaggat tgaatgataa atcgtaaaga aaactgcaac acctcgcgtg tagcacctgg
      781 ttagcgaact ttcgaaagta cgagtattgt atgatttcca gcagttatca attttacgta
      841 ttaatcagtg aatcaacaac tcgtcagcag actctctttg ctgttgaatt ttttggaacg
      901 tgtatagggc agaaattttt gttttgggac atggttggga cgccgtagta catacgaact
      961 ctgccgaata acgtatgata ttatttttta atctcttgga atacattata gaaacagaat
     1021 gctcttgggc gatttttagt gattgtttaa atagccctgt catgcagtta aagtggcggg
     1081 catcctcttc aaggatggtg atcctcaacc gcacccattt aggatgcgac tattgtggta
     1141 gaacaatgta aaccataatt aatctgcgtg cggatacatg cttcatagat aggatattct
     1201 ggtttgggtt gtgaatgtgt tggtcatcga gtggacaata aggtaagagg agtaactcct
     1261 gcaaaacaaa aaaagagttc aacaaaatca atcagttaaa aacacgagag atctgtataa
     1321 aaaagcatcg taaattctgt atttttcctt taaatataat atgttattga gttttgtaaa
     1381 atctaccagt gtggtacatg gatatcgata ccaccgccaa ttataagcta ggcggtgcac
     1441 agcaacacga tagcgtacgc ctcgatccgt gggtgtttat gttctcagca ggatatcgtt
     1501 tttaattccg cacaaaaacg accccgtaat atacggggtc aataaggaca tggtataaag
     1561 ggtattattt cttcgcttct acgccatcag tttcagaacg aattaaaaat ttctcagttg
     1621 tttgcggaat atgctgaatc agccagtcgg ccatttgctt ttcctcattt aaaatcgctt
     1681 cgatgatcgg aattgagact gtatcaccgg cattttttgc tgctgctaat agtgaggtgt
     1741 aacaggcgat ttcaaattgc tcgaagacat atccgctaat agagccttta actatttcat
     1801 cagaagggaa tataccaccg attgactgcc caagcgcagc cattttactc atggaatctt
     1861 taatgactga acgtgaaatg tcattacgat caagaatagt ttccagttga acaatctggt
     1921 ttttggtttc actaagatgt tgttcaatac gagcgcgtag ttcaggataa ttatctatac
     1981 ggctggccat ggattcaagc atagattcgg cttgcttttc cattgcgtgg gcgtcacgta
     2041 accagtcatg ataatgttca atacgattca tgaaaatgtc ctctttataa aatgactaaa
     2101 agttaaaatt catatttcag gctttatttt cggctttctt atttacgtta ttaagggcca
     2161 gatcagtcag tttgatgtcg gtggcctttt cttcttccag ggtttctttc agaagcttcg
     2221 ctgctttacg gtaacctaat tgttcagcca gcgtcgctaa tgtcccgtaa ctggcaatct
     2281 cataatgctc gactttctgc gctgcggcaa tcagtgcggc atcacgcact tcgtttttct
     2341 cggtactttc gatgacctca ttagcttctt caataagacc ttccattgcc acacatttca
     2401 tgcgcttaat tttcaggttc gattcggact ccacaacttg atcaatacgt tcaatctgtc
     2461 catgagtttc ctcgaggtgc gcatgaaaag cctgacttaa tttttcattt gatgttgctc
     2521 ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc gctgtaggta tctgaaagca
     2581 ggtgaataaa tacatcttca atggtcttca tattcatatt tttctccagt gaaatcactg
     2641 cgagccatac ggcccgcaga tgcaattatc tttatcagct gatattaata attaacggtg
     2701 aatcaggatt tgcctgattt attaccaccg ctttgttgac cgcctttttt acccgcttca
     2761 gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat gctgaccgcc tttacggcct
     2821 gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg aaccaccacg atgttcggcc
     2881 atgttatttc tcccgttgcg ttgcattgtt tcattaatat gagtgttgtg tgtcgacact
     2941 cattaaaatt agtcgctaat gagaattagt caaattaagc gcaacgagaa gatagaggga
     3001 aaatatattt tgaggaacat tctggatata ttaacaatta cctgaggaat aagtgactta
     3061 gagaaaatta ttgattttac tggtgttatg ttgcgggatt aatttgtttg attatattac
     3121 tgttgggcgg aaaaatgacg taagttgacg ctcgaccggg gtaagcgaaa cggtaaaaag
     3181 ataaatatta aatgaattta ggatttttcc ggcttcatta aagaaagtta aaatgccgcc
     3241 agcggaactg gcggctgtgg gattaactgc gcgtcgccgc tttcatcggt tgtacaaaaa
     3301 ctttcagtgc cgccagcatt ttctctggct cattaatatg ttgctcgatg attttaacaa
     3361 tggccgaacc agaaatcgcg cccgcagctc ctgcatcaat cgctgctttt acctgatccg
     3421 gggcggaaat accaaatccc tgcaatggag gtgcagcgtt gtactctttc agcttcgcaa
     3481 ccagatgatt gaggggtaac gcggcgcggt tttctgcgcc ggtcacgcct gctcgtgaca
     3541 gcaaataggt gtaaccacga ccgtaagagg ctatctggcg cagcaggtcg tcatcggcat
     3601 ttggcgggca gatgaagata ggtgcgacat tatgacgcaa cgcggcctgg cggaagggcg
     3661 cggactcttc aactggcaca tcggcaacca gcaccgaatc gacgccgact ttttcgcact
     3721 gggcataaaa ctcatcaatg cctttgttaa acaccagatt ggcatacatc aacaggccaa
     3781 tgggaatggt cgggtgtttc tggcgaatca gtgccagcat ttcaaaacat tgtgccggag
     3841 tcacacctgc cgcaaaggcg cgcagagtgg cgttttgaat cgtcgggcca tccgccagtg
     3901 ggtcggagaa ggggatacct aactccagcg cgtcagcacc ggcttcaatt agcgtatcga
     3961 taattttcaa tgactgctca atgcccggat caccgagcgt gacgaaagga acgaatgcgc
     4021 cttctttgcg ctccttcaac tgggcaaaca gagattcgta gcgttccatc agatttcccc
     4081 tcgtgctttc aaaatatcgt gaacggtgaa gatgtcttta tcgccgcgac cggaaaggtt
     4141 aaccaccagt agctgctctt tatccgggtt ttcgcgcatc attttcaacg catgggccag
     4201 ggcgtgggag gattccagcg ccgggatgat cccttcgtgc aggcacagcg ttttgaaggc
     4261 ttcaagggct tcatcatcgg taatagacac gtaatcagcg cgtccagtgc tgttaagata
     4321 cgcgtgttgt gggccgacag acgggaaatc cagtccggcg gagatggagt aagattcttc
     4381 aatctgcccg tcttcggttt gcatcatcgg cgctttcata ccgaaataga tacccacgcg
     4441 accatgtttt agcggtgcgc cgtgctcgcc agtttcgata ccgtgaccac ctggctccac
     4501 accaatcagg ccgacgttgg tttcattgat gaaatcagca aacatgccga tggcattcga
     4561 accgccgcca acacaggcga taacggcatc cggcaggcga ccttctcttt ccagaatctg
     4621 cgctttggtt tcttcgccaa tcatccgctg aaactcacgc acaatggtcg gataaggatg
     4681 cgggccagct gcggtgccca gcatatagtg cgcggtttcg taactaccgg accagtcgcg
     4741 cagcgcctcg ttacaggcat ctttcagcgt cgcggaaccg ctatgcaccg ggatcacttc
     4801 cgcacccatt aagcgcatac gaaaaacgtt aggcgactgg cgttcaacgt ctttggcacc
     4861 catataaata cggcatttca ggccgagcag ggcgctggca agggccgacg ccacgccatg
     4921 ctgaccggca ccggtttcgg cgatgatttc ggttttaccc atccgcttcg ccagcaacgc
     4981 ctgccccagc acctggttag ttttatgcgc gccgccgtgc agcaaatctt cacgcttgag
     5041 atacagcgta gtgttcgtcc cggctgtaat gttctggcat ttggtcagcg cggttggacg
     5101 cccggcatag tttttcagca ggtcgttgaa ctgagcctga aattcaggat ctttttgcgc
     5161 actgacaaaa gcttcttcca gctggcgcag agcaggcatc aggatttgtg gcacgtacat
     5221 gccgccaaac tcaccaaaat aggggttaag taatgttgtc attgttcctt tccttaatat
     5281 gcgcgcagcg tctggaaaac cgaggccaaa agacgtgcgt ctttgatgcc cggttgcgac
     5341 tctacagcag aattaaaatc aagtccggcg cagccggttt gtgccgcttc cacgcagtta
     5401 tctgcgccta agccccccgc cagcagaacg ttgccaagcg attgaccatt taatagtgac
     5461 cagtcaaaac gttgcccgct tccaccctgg ccgttgtcta aaacatattt atcaacgtgc
     5521 tgaaactcgc gggcgggcag ggtttcaccg acgcttaatg ctttccagat ggcaacatgt
     5581 gctggcagag cttcacgcag cgtatcgata tacagctgtt cttcattacc atgcagttgc
     5641 actgccgcca gcgataacac cttagctttg tccaccacat cggcaatatc gtgattgcgg
     5701 aacacgccaa catactgcaa cggtgccgca gccatcactt cctgcgcctg ttcaacgttg
     5761 acgcaacgcg gtgatgtcgc aacaaaaatc aacccaccgt aaatcgcgcc cgcgtcataa
     5821 gctgctttag catcttgccc acgcgtcagg ccacatactt tattctcacc cagcaacacc
     5881 cggcgcacgg cggcgtgcaa atcgtcatgg gccatcaacg ccgaaccaat cagaaaaccg
     5941 ttagcgaagt ggcttaactc gcgcacctga gcgtaagtat tgatgccgga ttcgctgatt
     6001 accgtcacgt tgtgccccag tttcggcgca agctcgcggg tacggttgag atcaatcgac
     6061 aaatcacgca gatcgcggtt gttgatgcca acgacctttg cccccaatgc aatggcacgc
     6121 tccagttcct cttcattact gacttcggtc agcacaccca tctccagact gtgggcgacg
     6181 gcggcaagct ggcgatattg ttcgtcatcc agtactgaaa gcattaataa gcaggcatca
     6241 gcctggtaat agcgcgccag atagatctgg taaggatcga taatgaagtc tttacataaa
     6301 atcggctgcg gggcgatttg gctgacgatg gggaggaaat caaagctccc ctgaaaatat
     6361 ttctcatcag tcagcaccga aattgccgaa gcgtaatgtt tataaatggc ggcaatgcgt
     6421 gctggatcga aatcatcacg gatcacgcct tttgacggcg acgatttctt gcactccaga
     6481 ataaacgccg tgcgcgcacc ctgtagcgca tcataaaaat gtcgcgtgct cggctgaacc
     6541 tcattctgaa aactggccag cggttgctgc tgtttgcggg tttctaccca aatcgccttg
     6601 tctgcgacga ttttcgctaa aacggtttgc atcatttacc ctcgtgccgc cagtgcggta
     6661 actctgtcgt aagcggaacc actgcgcagt acctcaagaa cggtttgcgc attggcttgc
     6721 agatcttcat ggccatgcag gcgcattaac atggcgacgt tcgcagcgac ggctgcttca
     6781 tgggcggcgt cgcctttacc ttgtaacaag cgtgttaaaa tgtcacggtt ttcttccggt
     6841 gttccgcctg ccagttgctc ctggtggtag ggagtcaggc caaaatcttc agcggtcaat
     6901 tgatagctct taatttcgcc gtcatgcagc tcggcaacga ttgtcggcgc gtgtaatgaa
     6961 acttcatcca tcccgccgct gtgcaccacc gccgcgcgtt gataccccag cacgcgcaag
     7021 gtttcggcaa tcggcagcac cagttccgga ctataaacac caattaacgc cagcggcgga
     7081 tgcgccgggt taatcaatgg ccccagcaca ttgaacaggg tgcgggtttt cagttgctgg
     7141 cgaaccggca tcgcgtggcg gaatccggtg tgatacttcg gcgcaaagag gaaacataca
     7201 cctaactcat ccagcgcctg gcgcgattta tcggcgttca tatcaagatt aataccgaac
     7261 gccgccagca gatcggacga accagattta ctggagacgc tacggttgcc gtgtttcgcc
     7321 actttcagcc cacaggccgc ggcgacaaac gcactggcgg tagaaatatt gatactgttg
     7381 ctgccgtcac cgccagtacc gacgatatca gcaaacagat aatccgggcg cgggaacggc
     7441 gcggcgtttt ccagtagcgc ggttgctgct ccggcgatct cgttcgggtg ctcaccgcga
     7501 attttcatgc tcaccagcgc cgccgccagt tgttccggct tcagctcgcc acgcaccacc
     7561 gctgaaaaca gctggtggct ttcttgttgg ctaagcgtct gcgcctgata cagtttttcc
     7621 agaatcggtt gcagcgtgtt ggctggctct agtttctgct gcgcccaggc cagcgtttgt
     7681 tccagcaggc gagcgccctg ggtggtgaga atggattccg gatggaactg gaatccacaa
     7741 acgcgatccg catcgtgacg tactgccatc accatgccat taaaatgggc gttgatggtt
     7801 aaaccggccg gaatgttact gccaaccagc gagtgataac gcgccaccgg cagcgggttt
     7861 gttaatccgg caaacatcgc ctgaccgtca tgttcaatgc tcgacgcttt accgtgaaga
     7921 atttcgcccg cctgaccgac atagcccccg taagcttcga caattgcctg atgtccgagg
     7981 caaatgccaa taatgggcag cttgccacgc aagcgggtga ggagttccgg catacaaccg
     8041 gcttcgctcg gcacaccggg gccaggagaa agcatcagta ccggattgct catcgtcgcc
     8101 aggcgttcaa ttaaggtctg cgccggaata tggttgcggt aaatcaccac attatgccca
     8161 ttgctgcgca actgatctgc caggttgtac gtaaaagagt cgatattatc gagcagcaga
     8221 atgtcagcca tcagaaagtc tcctgtgcat gatgcgcggt ggcaatagcg cgcagtacag
     8281 cgcgggcttt attacgggtt tcgtcggctt ccgactgcgg aacagaatca aggactacgc
     8341 cagcaccggc ttgcacggtg gcgataccgt tttccaccag cgccgagcgg atcacaatgc
     8401 aggtgtcgag atcgccatgc gcggtgaaat aacctaccgc gccgccgtag ctgccgcggc
     8461 gacgaccttc cgcctcggca attaactgca tagcgcgtac tttcggcgca ccacttaacg
     8521 ttcccatatt catacaggcg cggtaagcgt gcagggcgtc gagatcgtgg cgcagctcac
     8581 cgaccacgcg ggagaccaag tgcatcacgt aagagtaacg gtcaactttg gtgaggtcgg
     8641 cgacgtagcg gctgccgggg gtgcaaatgc gtgccagatc attacgggcg agatccacca
     8701 gcatcagatg ttcagaaagc tctttatgat cggtacgcat ttccagttcg atgcggctgt
     8761 cgaggtctct gtccagtgaa ccatcggcac gacgaccgcg cggtcgtgtt ccggcaatcg
     8821 ggtagatctc aatctggcgg ctggtggcgt catacttgag cgaactttcc ggcgacgcgc
     8881 caaacagggt gaaatcatta tcctgcataa aaaacatgta cgggctggga ttactctttt
     8941 tcagcacgta ataggccgcc agcggtgacg ggcagggcag agagaaacgg cgagatggca
     9001 ccacctggaa aatttctccg gcgcgaatcg ctttttgcaa caaacgcact acgccaccga
     9061 actcttcatc gctctggtta cattcacaac gcatatgcgg cacggaaacc accggcagcg
     9121 gtggcgcggt ttcggtcagt tgctggcgaa gatcgttcag gcgagcagtg agacgttgtt
     9181 tttcttcttc attcggagca aacaggctgg cctgaatgcg ggtgcttttt ttctgatggt
     9241 caatcaccat cagcgtttca gcgagataaa aacagaaatc aggacagtta ttttccgctg
     9301 acaattgcgg taaatcttca aaccccgcca caaggtcata agagaacagg ccgccgaaga
     9361 acattgcttc tcgttcttcc ttcggtacat tcagcatatt ctgcaataag cggaaagcgt
     9421 caaaaaccga aagggagcat aagcgagcgt cttcatccag cagtggactg acagggggga
     9481 agcgcagcac acggcagttt ggtaattgtt cattttccat acccgcaggc agggcgttat
     9541 ccagtagtgt cagcagggct tcgccgttgc cggaaagtgc ctggattgtg acagtgtcac
     9601 ctaaagctgt aatgcgcagc gcactgtcta ccagcagcag gctttttaaa tcatctttgc
     9661 tgtcgatatc tgcggattcc agcagcagcg ttgccggacg atccccacac aactggtgaa
     9721 aaagcgcggt gggattgtcg cgataagcgc cttcgcaggt tagcagttcg agagtcggtt
     9781 tttgtgtttg cattgttatt ctctaatttt gttcaaaaaa aagcccgctc attaggcggg
     9841 ctgggtatct gattgcttta cgcatggtga atacactgcc cgtttcagga agtgcgccac
     9901 caacctttca gtacgaaaat tgctttcatt gtcgataccc tttttacgtg aacttgcgta
     9961 ctagttaact agttcgatga ttaattgtca atagctcatt tcagaatatt tgccagaacc
    10021 gttatgatgt cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa
    10081 ttatgcagtg atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc
    10141 agaagcattg gtgcaccgtg cagtcgagat gcgcgtcggc accctggcga tcaccgacca
    10201 tgacaccaca gctgcaatcg cgccagcaag agaggaaatt tcacgttcgg gactggcgtt
    10261 gaatcttatt cccggcgtgg aaatttccac ggtctgggaa aatcatgaaa ttcatattgt
    10321 cgggctgaat attgatatta ctcatccgct aatgtgtgag ttcctcgcgc agcagacaga
    10381 acggcgcaat cagcgggcgc agctgattgc cgaaagactt gaaaaagcgc aaatacctgg
    10441 ggcgctggaa ggcgcacaac gactggcgca gggcggcgca gtgacgcgcg gtcattttgc
    10501 gcgtttcctt gttgagtgcg gcaaagccag ttcaatggcg gatgtcttta aaaagtatct
    10561 ggcgcgcggg aaaaccggat acgttccgcc acagtggtgt acaatagaac aagctattga
    10621 tgtcattcat cattctggcg gtaaggcggt gctagctcat cctgggcggt acaatcttag
    10681 tgctaaatgg ctgaaaagat tggttgcgca ttttgccgaa caccacggtg acgcgatgga
    10741 agtcgcgcag tgccagcaat cgcccaatga acgtacccag ctggcggccc ttgcgcgtca
    10801 gcatcattta tgggcatcac aaggatctga ttttcatcag ccatgcccgt ggatcgaact
    10861 gggtcgtaaa ctctggctgc ccgcaggcgt tgaaggcgtc tggcagctat gggaacagcc
    10921 gcagaacacc acagagaggg aattatgagc cagttttttt atattcatcc tgataaccca
    10981 cagcaacgtc tgatcaacca ggcggtggag atcgtgcgta aaggcggggt gattgtttat
    11041 ccaactgatt ccggctatgc gctcggctgt aaaattgaag ataaaaacgc gatggagcgt
    11101 atttgtcgta ttcgccagct gccggacggt cacaacttta ccctgatgtg tcgcgatctt
    11161 tctgaactct cgacctattc atttgttgat aacgttgcgt ttcgtttaat gaaaaacaac
    11221 acgccgggca actatacctt catcttgaag gggacgaagg aagtgccacg ccgtctgttg
    11281 caggaaaaac gcaaaaccat cggtatgcgt gtgccttcta acccgattgc gcaggcgttg
    11341 cttgaagcac tgggcgaacc gatgctttcc acttcgctaa tgctgccagg cagcgaattt
    11401 accgaatccg atcccgaaga aattaaagac cgtctggaaa aacaggtaga tttgattatt
    11461 cacggcggct atctcgggca gaaaccgaca acggttattg atcttaccga tgataagccg
    11521 gtcgtggtgc gtgaaggcgt aggtgatgtg aagcctttct tataagtgcg taactctgta
    11581 tactacgcgg ccttaaaatg gcgctatacg gcgccattga ttctcctgtt cgacgcctgt
    11641 gaaggcgaca ctaaaaggaa gctctatgag cgaaaaatta cagaaagtgc tggcccgcgc
    11701 tggccacggc tcccgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
    11761 tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctgggctga aaattcgtat
    11821 cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
    11881 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc cgacggtgtt
    11941 tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa
    12001 cacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcatcc
    12061 aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt ggtcaggttg acgacgcgaa
    12121 actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt ccggcagctt ttaaaaccat
    12181 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg
    12241 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg
    12301 tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct
    12361 ggacctcgcc cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc
    12421 taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt
    12481 gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc ggacgtaata acggttaatc
    12541 aaagtatgcc ggacgtcata tccggcattt ttacagatta gtaatcaatc cctatctgcg
    12601 cttttacacc ggcatcaaac gcatgtttga cggggcgtaa ttcacttacc gtatctgcca
    12661 gttcaagaat atcccgatga caaccacgac ccgtgataat caccgtctgt tgatgtggac
    12721 gttcatttaa cgcctgcacc acttcttcca gtggcaaata gtcatacgcc accatatacg
    12781 tcagttcatc aagcaaaacc atatccagtg aggaatcagc aagcatccgc tttgcatgtt
    12841 gccagacttc gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc caggtaaagc
    12901 ccgttgccat cacctgaaac tcaacgccat gtggctccag cagattgcgt tcgccattag
    12961 gccaggtgcc tttaataaac tgcacgacac ctactttttt tccgtgacca actgcgcgtg
    13021 ttgccgtacc aaatgccgcg gtggttttgc cttttccatt gccggtaaag acgatgataa
    13081 taccgcgttc atcctgggcc tgggccacac gagcatctac tttttctttc actcgctgct
    13141 gacgctgttg gtagcgttca tcactcattg ggaaattcct ggtttacggc ccggttgggc
    13201 gtcaaaggtc atgccggttt tacggcggct gtcatcgccc atcagccaga ggtagagcgg
    13261 catgatatca gcgggtgttt taagtttctg tggatcttcg gtcgggaagg cgctggcacg
    13321 cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg cgcaggcgct gctgatattc
    13381 atcggccagt acctgcatca tcccttcggt ggcaaatttc gacgctgcat atgcacccca
    13441 gttagctcgt ccctgacgtc caacgcttga tgaagtaaag accagtgaac cggcgtccga
    13501 tttgagtaat aaaggaagca gcgcctgggt gagcaaaaag gtggcattaa cgttgacctg
    13561 catgacgtcc tgccagacct gcggattttg ttcgctcatt gggcaaacat cgccgagcaa
    13621 tccggcatta tgcaaaacac catccagacg cggataatta acggtaatgc gctgtgccag
    13681 ttgttggcaa tcttcggacg tgcaggtcag caaatcgaga ataaaccact gtggctgacg
    13741 tccagtttct tcgtttatgt ggctggctac ctgacgtaat ttttcttcat tacggcccaa
    13801 cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg gcttcacggc caatcccatc
    13861 gctggctccc gtcaccagga taatgcgatc attgagtaaa tcttgttttg gctggtaatg
    13921 catggctact cctcaacgac gttgtctgtc gcgctgccag tgtactttat gactttgggg
    13981 ctttatgcct gaaacagaag ggcatttcaa tcagcctggg gcgataacac cgcaaaatgg
    14041 atactttgtc atactttcgc tgcaataaca tctctgcgag acggcttaac atgcctgttg
    14101 taaactgtga gccaaagcgt tgtttaacca aggtggggac tcgtggaatt gttgtctgaa
    14161 tatggtttgt ttttggcgaa aatcgttacc gttgtgctag cgattgcggc gattgccgcc
    14221 attattgtca atgttgctca acgtaataaa cgccagcgtg gcgagttacg ggtcaacaat
    14281 ctcagcgaac agtataagga gatgaaagaa gaactggccg cggcgctgat ggactcacat
    14341 cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca agcaagaagc gaaagcagca
    14401 aaagcgaaag ccaaactggg cgaggtggca actgacagta aacctcgcgt ctgggtgctg
    14461 gattttaaag gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga gataacggcg
    14521 gtactcgcag cattcaaacc gcaggatcag gttgtgctcc gtctggaaag ccctggtggc
    14581 atggtgcatg gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga taaaaacatt
    14641 cctttaactg ttacggtaga caaagtcgct gccagcggcg gttacatgat ggcctgtgtg
    14701 gcggacaaaa ttgtttccgc accgtttgct attgtgggtt ccattggggt ggtggcgcaa
    14761 atgcccaact ttaaccgctt cctgaaaagc aaagatattg atatcgaact gcacactgcc
    14821 gggcagtata agcgtactct gaccttgctg ggtgaaaata ccgaagaagg gcgggagaaa
    14881 ttccgcgaag agttgaacga aacgcatcag ttgtttaaag attttgtgaa gcgtatgcgt
    14941 ccgtctctgg atattgaaca ggtggcaacg ggtgaacact ggtacggaca acaggcggta
    15001 gagaaaggcc tggttgatga aatcaacacc agtgatgaag ttattcttag cctgatggaa
    15061 ggccgtgaag tggttaatgt acgctatatg cagcgtaaac gactcattga ccgattcacc
    15121 ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct ggtggcagcg gggtcaaaag
    15181 ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta atcaaccaga
    15241 tgatattttt ctgaaagcac atgggccagg tgtttgaaca tattaaacac cgcggtgctt
    15301 ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca
    15361 ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg
    15421 attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc tttgttcatt
    15481 atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc
    15541 acgctggtgg taagagcgcc ttactggtaa ctttggattt tgcatgctaa taaagttgcg
    15601 tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga
    15661 cattcgacca aaattccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc
    15721 acctgaattt tcgtgaacag agtcacgaca aggggttgat atccgcagag aacgagtcca
    15781 tatcggtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc
    15841 aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc
    15901 aaaacgatca acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc
    15961 cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac ctccaccaag
    16021 acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac
    16081 ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtctct
    16141 gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc
    16201 gaaggggaag ccattgcatg gcacctgcgg gaagtgattg ggggtgatga tgcgcgctat
    16261 agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcatt taataaaccg
    16321 ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg
    16381 gtggggtata tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct gtctgccggt
    16441 cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc
    16501 gtgccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg
    16561 gcgttacagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact
    16621 caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa
    16681 ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc
    16741 acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca
    16801 ggctatatca cttacatgcg taccgactcc actaacctga gtcaggacgc ggtaaatatg
    16861 gttcgcggtt atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag
    16921 tacgccagca aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat
    16981 gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc agaaactgta ccagttaatc
    17041 tggcgtcagt tcgtagcctg ccagatgacc ccagcgaaat atgactccac gacgctgacc
    17101 gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta ttttgcgttt tgatggctgg
    17161 acaaaagtga tgcctgcgtt gcgtaaaggc gatgaagatc gcatcttacc agcagttaat
    17221 aaaggcgatg ctctgacgct cgttgaactt acaccagccc agcactttac caagccgcca
    17281 gcccgtttca gtgaagcatc gctggttaaa gagctggaaa aacgtggtat cggtcgtccg
    17341 tctacttatg cgtcgatcat ttcgaccatt caggatcgtg gctacgtgcg agtagaaaat
    17401 cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg atcgccttga agaaaatttc
    17461 cgcgagttaa tgaactacga ctttaccgcg cagatggaaa acagcctcga ccaggtggca
    17521 aatcacgaag cagagtggaa agctgtactg gatcacttct tctcggattt cacccagcag
    17581 ttagataaag ctgaaaaaga tccggaagag ggtggtatgc gcccgaacca gatggttctg
    17641 accagcattg actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc gagcaccggg
    17701 gtattccttg gctgttctgg ctatgcgctg ccgccgaaag agcgttgcaa aaccaccatt
    17761 aacctggtgc cggaaaacga agtgctgaac gtgctggaag gcgaagatgc tgaaaccaac
    17821 gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag ctatctcatc
    17881 gatccgaaac gtaagttgca tgtctgtggt aataacccaa cctgcgacgg ttacgagatc
    17941 gaagagggcg aattccgcat taaaggttat gacggcccga tcgttgagtg tgaaaaatgt
    18001 ggctctgaaa tgcacctgaa aatggggcgt ttcggtaaat acatggcctg caccaacgaa
    18061 gagtgtaaaa acacacgtaa gattttacgt aacggcgaag tggcaccacc gaaagaagat
    18121 ccggtgccat tacctgagct gccgtgcgaa aaatcagatg cttatttcgt gctgcgtgac
    18181 ggtgctgccg gtgtgttcct ggctgccaac actttcccga aatcgcgtga aacgcgtgcg
    18241 ccactggtgg aagagcttta tcgcttccgc gaccgtctgc cggaaaaact gcgttatctg
    18301 gccgatgcgc cacagcagga tccggaaggt aataagacca tggttcgctt tagccgtaaa
    18361 accaaacagc aatatgtctc ttcggaaaaa gacggaaagg cgactggctg gtcagcattt
    18421 tatgttgatg gcaaatgggt tgaaggaaaa aaataacctt taattctgtc aggtttttat
    18481 aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat
    18541 acactaaggc tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt
    18601 attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt
    18661 ttaacatgaa attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg
    18721 tctcatcaac agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa
    18781 tgctggaaga cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg
    18841 taacgccagc aggacaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg
    18901 ccataaaatc ggttgccggt gagcacacct ggccggataa aggctcgctg tatatcgcca
    18961 ccacgcatac ccaggcacgc tacgcgttac caaacgttat caaaggcttt attgagcgtt
    19021 atccccgcgt ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct
    19081 ctaaaggcaa tgctgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag
    19141 tgatgttacc gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg
    19201 caggcaaaaa agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct
    19261 tcggctttac cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc
    19321 gtatcgtctt cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg
    19381 gggtaggggt cattgccagc atggcggtgg atccggtcgc cgatcccgac cttgtgcgtg
    19441 ttgatgctca cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt
    19501 tcttgcgcag ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg
    19561 tcgttgatgc ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata
    19621 taaaactgcc ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt
    19681 gccggaatat attctctttt gttacttatt tctataccgt gcacaatatt tttatccctc
    19741 atatttatag ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaactccag
    19801 ttttcaaata tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat
    19861 tcgctggatt tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc
    19921 aatcgttcaa cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg
    19981 gaaatcagga accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc
    20041 ttggttctgc acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac
    20101 tatggtcggt caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac
    20161 agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca
    20221 gcaagccttt atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt
    20281 cttattaccc atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg
    20341 agttatctca acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg
    20401 aaatattggt cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc
    20461 tggatcatta ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac
    20521 aaggtttctc ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt
    20581 atctttaata ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc
    20641 tatgtcgtca accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta
    20701 ccactactac agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa
    20761 gtcactcaaa gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga
    20821 agaggatatc cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc
    20881 ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc gtacctgccg ttgttgatct
    20941 ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct
    21001 ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga
    21061 ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct
    21121 gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg
    21181 ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga
    21241 atggattgct tatccggata cactcgttgg tactgactcg cacaccacta tgatca
//
LOCUS       DAZFDP010000076        20827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFDP010000076 DAZFDP010000000
VERSION     DAZFDP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(141..1790)
                     /gene="actP"
                     /locus_tag="ACHJ1G_002880"
     CDS             complement(141..1790)
                     /gene="actP"
                     /locus_tag="ACHJ1G_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4764705.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1787..2101)
                     /gene="yjcH"
                     /locus_tag="ACHJ1G_002881"
     CDS             complement(1787..2101)
                     /gene="yjcH"
                     /locus_tag="ACHJ1G_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4764706.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2302..4260)
                     /gene="acs"
                     /locus_tag="ACHJ1G_002882"
     CDS             complement(2302..4260)
                     /gene="acs"
                     /locus_tag="ACHJ1G_002882"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4764707.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4652..6088
                     /gene="nrfA"
                     /locus_tag="ACHJ1G_002883"
     CDS             4652..6088
                     /gene="nrfA"
                     /locus_tag="ACHJ1G_002883"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4764708.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6133..6699
                     /gene="nrfB"
                     /locus_tag="ACHJ1G_002884"
     CDS             6133..6699
                     /gene="nrfB"
                     /locus_tag="ACHJ1G_002884"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4764709.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6696..7367
                     /gene="nrfC"
                     /locus_tag="ACHJ1G_002885"
     CDS             6696..7367
                     /gene="nrfC"
                     /locus_tag="ACHJ1G_002885"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4764710.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7364..8320
                     /gene="nrfD"
                     /locus_tag="ACHJ1G_002886"
     CDS             7364..8320
                     /gene="nrfD"
                     /locus_tag="ACHJ1G_002886"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4764711.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8340..10058
                     /gene="nrfE"
                     /locus_tag="ACHJ1G_002887"
     CDS             8340..10058
                     /gene="nrfE"
                     /locus_tag="ACHJ1G_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4764712.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10051..10434
                     /gene="nrfF"
                     /locus_tag="ACHJ1G_002888"
     CDS             10051..10434
                     /gene="nrfF"
                     /locus_tag="ACHJ1G_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4764713.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLRLMALILWRVRAKR"
     gene            10431..11027
                     /gene="nrfG"
                     /locus_tag="ACHJ1G_002889"
     CDS             10431..11027
                     /gene="nrfG"
                     /locus_tag="ACHJ1G_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4764714.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11369..12682
                     /gene="gltP"
                     /locus_tag="ACHJ1G_002890"
     CDS             11369..12682
                     /gene="gltP"
                     /locus_tag="ACHJ1G_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4764715.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12760..13449)
                     /gene="yjcO"
                     /locus_tag="ACHJ1G_002891"
     CDS             complement(12760..13449)
                     /gene="yjcO"
                     /locus_tag="ACHJ1G_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4764716.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13543..15690)
                     /gene="fdhF"
                     /locus_tag="ACHJ1G_002892"
     CDS             complement(13543..15690)
                     /gene="fdhF"
                     /locus_tag="ACHJ1G_002892"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15271..15273),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO4764717.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15888..17354)
                     /gene="mdtP"
                     /locus_tag="ACHJ1G_002893"
     CDS             complement(15888..17354)
                     /gene="mdtP"
                     /locus_tag="ACHJ1G_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO4764718.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17351..19402)
                     /gene="mdtO"
                     /locus_tag="ACHJ1G_002894"
     CDS             complement(17351..19402)
                     /gene="mdtO"
                     /locus_tag="ACHJ1G_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO4764719.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19402..20433)
                     /gene="mdtN"
                     /locus_tag="ACHJ1G_002895"
     CDS             complement(19402..20433)
                     /gene="mdtN"
                     /locus_tag="ACHJ1G_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4764720.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20452..20727)
                     /gene="ytcA"
                     /locus_tag="ACHJ1G_002896"
     CDS             complement(20452..20727)
                     /gene="ytcA"
                     /locus_tag="ACHJ1G_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4764721.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4693 a         5520 c         5705 g         4909 t
ORIGIN      
        1 ttctctgcat tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt
       61 tgtttaatat tatgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg
      121 cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg
      181 gagcggataa actgtgcacg gaacagttca cgctcgcgcg caccttccgc tgagttatcg
      241 gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag cgccgggtat
      301 tcatacggga agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg
      361 atcatcagta ccactgcggt aatcagcccc agccagccac ccatcatcgc gccacgagtg
      421 gtcagtttcg accagtacat agaaagcaga atgatcggga agttacagct cgctgcgatg
      481 gcaaacgcca ggcctaccat gaaggcgatg ttctgattct caaacagcac gccgaggata
      541 atggcaatca cacctaaaat cagcacggtg attttcgata cccgcagttc ttcacgttcg
      601 gtcgcgcctt ttttgaagac gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc
      661 agcgtcagac cagcaaccac cgcaagaatg gtggcaaaag caaccgctga aataaaaccg
      721 aggaacaggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca
      781 atcagatggc ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg
      841 aagccgataa taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg
      901 ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag
      961 cccgccgtac caaacatcag tcccagaccc agagagagcg cggagatcgg atctttcacc
     1021 agcccgcccg gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac
     1081 agattgttga agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg
     1141 aacagcaaca gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac
     1201 aggacgtaca tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca
     1261 aacagcagct cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc
     1321 accaccagag aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag
     1381 gccacatcgg caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa
     1441 atgatcggcc agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta
     1501 aacaccagcg cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc
     1561 agcccgttct ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt
     1621 acgcgttttg atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata
     1681 ataatcgcct gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg
     1741 ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta
     1801 cctcatgcag gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga
     1861 taccggtgag aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga
     1921 cgctggtgtt cggattcagc ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa
     1981 taaagccgat ataaactgcc agcataataa tcgacaggat ggtggcaaac cgttgccgtt
     2041 tttcgactaa ctccctgaaa tgcgcattgt cttctatccg ctgataaatc gtaccattca
     2101 tcacagattc tccagaggta atgtagggat tgttttaatt cccgctccct tatgggagaa
     2161 ggttaacgct cgggtaaccc ttgccgaatg taggccggat aaggcgttat acgccgcatc
     2221 cggcaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga
     2281 ctcgcatccg gcaattgtgg gttacgatgg catcgcgata gcctgcttct cttcaagcag
     2341 cttctcgact acgccaggat cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc
     2401 cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg gagcgggttt taggcaggga
     2461 gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca atctctttac gcacccagtt
     2521 gcggacttct gcgtacagtt ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc
     2581 gtagatcgcc tgacctttaa tattgtgcgg aatacctact acggcggctt cggcaatctt
     2641 cggatgcgcc accagcgccg actcaatctc tgccgtcccc agacggtgac cggagacgtt
     2701 cagcacgtcg tccacacgcc cggtgatcca gtaatagcca tcttcatcgc gacgcgcgcc
     2761 gtcgccgctg aaatacatat ttttgaaggt ggagaagtag gtctgttcaa aacgttcgtg
     2821 atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca ggctgccttc
     2881 ggtagcgcct tccagcgggt taccttcgtt atcgaccagc gccggttgca cgccgaagaa
     2941 cggacgtgtt gccgaaccgg ctttcagctc ggtagcgcca ggcagcggcg tgatcatgaa
     3001 accgccagtt tcggtctgcc accaggtatc gaccaccgga catttctcgt tgccgatttt
     3061 tttccagtac cactcccacg cttccgggtt aattggctcg cccacggaac cgagaatgcg
     3121 cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct tccgccatca gcgcgcggat
     3181 cgccgtgggt gcggtataga gaatattgac ctgatgcttg tccaccacct gcgccatacg
     3241 ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc gtggtcgcac cgcaggccag
     3301 cgggccgtac agcaagtaac tgtgtccggt cacccagccc acatcggcgg tgcaccagta
     3361 gatatcaccc ggatgataat caaagacata tttaaaggtc agcgccgcgt acaccagata
     3421 accgccggta gtatgcagca caccttttgg cttaccggta gaaccggagg tgtagagaat
     3481 aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc tgatcgctcg cttgctcaac
     3541 caggtcgtgc caccacaggt cgcgcccttc ctgccagtca attttcccgc cagtacgctt
     3601 cagtaccacc acatgctcta cgctggtgac gttcgggttt ttcagcgcgt catcaacgtt
     3661 tttcttcagc ggaatactgc gcccggcacg cacaccttcg tcggaagtga tcaccagtcg
     3721 tgagttggaa tcaataatgc gcccggcaac ggcttccggc gagaagccgc cgaaaatcac
     3781 cgaatgcacc gcgccaatgc gggcgcaggc cagcatcgca accgcggctt ccggcaccat
     3841 cggcatataa atcgccacca catcaccttt tttaatgccc agctcgagca gggtattggc
     3901 gaagcggcac acgtcgcggt gcagctcttt atagctgata tgtttgctct ggctggcgtc
     3961 gtcgccttcc cagatgatgg cggtgcgatc gccgttttct tgcagatggc ggtcaaggca
     4021 gtttgccgcc agattcagcg tgccgtcctc gtaccattta atggacacat taccgggggc
     4081 aaaggaggta tttttcacct tctggtacgg tttgatccag tcgagaattt ttccctgttc
     4141 gccccagaag gtatcaggta cgttaataga ttgttgatac atcgcctcgt actgctgagg
     4201 gtttatcagg caacggtctg cgatgttggc aggaatggtg tgtttgtgaa tttggctcat
     4261 gcttttgttc tccttgtagg atgttaataa tatgtggcat aagcgttaaa tgtaggggta
     4321 ttggcagttt tgtttagtat ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa
     4381 acggcagggt aatttttgaa ggtcaccaac aaaagttgat taattctttg aggaacatgc
     4441 agttatgcat gctgttgaaa aagaggaaga tactgactaa ctctaaagtg gtattttaca
     4501 tgcacttaca attgattaaa gacaacattc acagtgtggt tatatgttac acataggggc
     4561 gagcaatgtc atgacagtgt aggtgcggtt actgtcgtga aaacaataaa aaccgccatt
     4621 gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg
     4681 tatcttcagc ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc
     4741 agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc
     4801 cgatcaatat ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc
     4861 ggaagatcca cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa
     4921 gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc
     4981 gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt
     5041 ggcgcgtctg atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg
     5101 cggcccggaa atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga
     5161 gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga
     5221 agccattggt aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg
     5281 tcagtgccat gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga
     5341 tgacggcatg aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg
     5401 gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg
     5461 gacagcgggc attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt
     5521 gcagaacgcc gaaggcaaac tctacaccga ccataaaatt ggtaatccgt ttgataactt
     5581 cgcccagact tgtgcgaact gccataccca ggacaaagct gctttgcaaa aagtggtcgc
     5641 ggaacgtaag cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc
     5701 tcacttcgaa gcgaaagcag cgctggatgc aggcgcgacg gaagccgaaa tgaagccgat
     5761 tcaggacgat atccgtcatg cccagtggcg ttgggatctg gcgatcgctt cccacggtat
     5821 tcatatgcac gcaccggaag aaggtctacg gatgctcggt acggcgatgg ataaagcggc
     5881 ggatgcacgc accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca
     5941 gatcccggat atctcgacca aagagaaagc ccagcaggcc attggcctga acatggaaca
     6001 aatcaaggcc gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc
     6061 acgtaaaaat ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac
     6121 aatggagtga atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc
     6181 ttgttgtgga gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc
     6241 tacgaagtta cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat
     6301 accgaaggta tgcatggaaa acatgcttcc gttatcaacc cgaataacaa actgccggtc
     6361 acctgcacca actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg
     6421 atgcgcttta acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt
     6481 catctgccgg aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg
     6541 gcgtgcgcca gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac
     6601 aaaggacgga ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt
     6661 aacccggcgt ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc
     6721 tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa
     6781 cgttgaatat caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct
     6841 gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc
     6901 tgacgattat tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc
     6961 gtaagtcttg ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt
     7021 cttttcgtga tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc
     7081 agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg
     7141 cggacaaatg cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgcg
     7201 ttgaggcttg cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga
     7261 tttcgcaact gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac
     7321 cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc
     7381 atttcatttt gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg
     7441 tatttctgcc ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg
     7501 cggtgcagac agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct
     7561 cggtctgttg atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt
     7621 ccactacagt tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt
     7681 ggtgctggtg ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg
     7741 ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg
     7801 cggactggaa acattgatgc tggtgttggc ggtgttgttg ggggcttata ccggctttct
     7861 gctgtcggcg ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct
     7921 cttctccggc atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg
     7981 cagtaacccg cattccacgg aagcgcagtt tgtacaccgt atggaaatcc ccgtggtatg
     8041 gggtgaaatc ttcctgctgg tggcattttt tgtcggtctg gcgctgggcg atgacggtaa
     8101 agtgcgtgcg ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg
     8161 tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc
     8221 cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg
     8281 ctttttcatt ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc
     8341 tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg
     8401 tgttgacccc gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac
     8461 tcacttgcat cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga
     8521 cgtattgttt tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc
     8581 tgctgtcgtg ggaactcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc
     8641 tttgggtgct gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa
     8701 ccgatccgct atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac
     8761 tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc
     8821 gcgatctcaa tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc
     8881 ttggctatgg cggtttgatg gtagcggcga gcgtggcgct ggcgagttta ctgcgcggcg
     8941 agtttgatgg tgcctgcgcc agaatttgtt ggcgctgggc actacctggc tggagcgcat
     9001 taacggcggg gatcatcctc ggttcctggt gggcctattg cgaactgggc tggggcggct
     9061 ggtggttctg ggatccggtg gaaaacgcct ctttattacc ctggctttct gccactgcgc
     9121 tgctgcacag tttgtccctg acacgccagc gggagatttt ccgccactgg tcgctgttgc
     9181 tggcgatagt aactctgatg ctgtcgctac tgggtacctt aattgtccgt tctggcattc
     9241 tggtttcggt tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt
     9301 ttgcactgat tagccttgcg tctctggctc tgtatggctg gcgagcgcgg gacggtggtg
     9361 cggtggtgcg tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt
     9421 tttgcgcagt gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggcctgc
     9481 tgggctgggg acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg
     9541 gcctgttgat gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc
     9601 ttccggcgct ggtagctcat gcgggcgtgc tgttatttgc cgcggggatc gtggtttcca
     9661 gcgtcagccg tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag
     9721 gatacacctt ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa
     9781 aagcgatagt ggcactgttt gaccatcagc aacgcattgg tgaactgatg ccggagcggc
     9841 gtttttacga agcacgtcgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc
     9901 atgactggta tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgcttgt
     9961 atgtacaaag cggtgtgcgc tggatctggg ggggaggatt gttgatgatt gcgggcgcat
    10021 tgttaagcgg atggcggggg aggaagcgcg atgaataaag gacttctcac gctactgctg
    10081 ttatttacct gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa
    10141 caacagcaac aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa
    10201 aacttactgg aatccaacgc gccggtggca gtcagtatgc gccatcaggt ttacagcatg
    10261 gtggcggagg ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat
    10321 tttgttcgct ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg
    10381 gtgttgttac ggctgatggc actgatcctc tggcgagtga gggcgaagcg atgaaagagc
    10441 ccaaaatacc ggtgaaaatg ctgacaacac tcacgatttt gatggtattt ctctgtgtcg
    10501 gcagttatct gttaagtcca aaatggcagg cggtacatgc ggaatatcag cgtcagcgcg
    10561 atccgctaca tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg
    10621 ataaaatccg cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc
    10681 tgtggcaaaa cgattacagc aattcgctgc tggcgtaccg tcaggcgttg caactacgtg
    10741 gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc
    10801 atatgaccgc ccagactcgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa
    10861 tcaccgctct gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca
    10921 tcgaactatg gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg
    10981 tcgagtcgat taatatggcg aaattgttgc agcggagatc agattaatca tctggtgatg
    11041 aagattatgt tattgagttg tataaaaaat aatcgaatga acatatgcca aaaataatca
    11101 ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca
    11161 ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa
    11221 agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa
    11281 ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga
    11341 acggcgctcc attgaggaag ttattctgat gaaaagtata aaattcagcc tggcctggca
    11401 gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga
    11461 cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct
    11521 gatcaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt
    11581 tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat
    11641 caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg
    11701 ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc
    11761 ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat
    11821 tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg tgctgtttgg
    11881 tctggggctt tcctccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc
    11941 catctctgaa accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt
    12001 gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc
    12061 gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat
    12121 tgtggcgcgc ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct
    12181 gattctggcg tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat
    12241 ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt
    12301 taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta
    12361 tggcattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc
    12421 gaaagggatt gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag
    12481 cgtaggtatt ccgctggaag gcctggcgtt tattgctggt gttgaccgta tcctcgacat
    12541 ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg
    12601 ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt
    12661 tgataaaact gcggatcaat aattgaagat tgccggggtt atccacccgg caatgtgtgg
    12721 atgcctgatg cgacgctggt gcgtcttatc aggcctacct taaccgttgg ttaatttttc
    12781 aaactcttca cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc
    12841 cttttgtttg ttcttctcga taaaaccttc ctcaccgttt aagaacatca ttcccgccca
    12901 gtactcggaa taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt
    12961 gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc
    13021 atcgacggcg gagtcattct ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt
    13081 tggataatcc ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc
    13141 ttctttactg cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag
    13201 actgaccgga ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct
    13261 gtcggcggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct
    13321 gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct
    13381 gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat
    13441 ttttttcatt ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga
    13501 taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc
    13561 gcgcaggcga gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg
    13621 atcggcgatc ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg
    13681 gcttaagttt tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta
    13741 aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg
    13801 cgagtgcacc caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt
    13861 gatttgtgcg tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat
    13921 cgaacggcaa gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc
    13981 ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag aactgtgcca gaccgttcgg
    14041 ggtatcaaac ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg
    14101 gcaaggccac tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc
    14161 cgggcacaga tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata
    14221 acccatacgg gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg
    14281 ttcaactgct ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg
    14341 ctcgccccac gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa
    14401 gatgtcctga acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc
    14461 cgcgtcagtt tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc
    14521 tgcgcggtgc ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc
    14581 ccaggctttg gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata
    14641 cgtatccggc agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg
    14701 gtttacaccc gcatgcggct taccgaggtt accggtcagc atcgcgaggc tggtcagaga
    14761 acgcacggtt tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc
    14821 gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt
    14881 gatatcttca accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc
    14941 ttctgtacgg gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc
    15001 catcgcattc aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat
    15061 gcgcgcggtt tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc
    15121 gttaattacg tgattcgcca cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa
    15181 cactaaatcg gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga
    15241 ttggtgcaga cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt
    15301 ggtaccaata acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc
    15361 acgcgaggag ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag
    15421 gcgctcggca acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg
    15481 ctggcgacgg atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa
    15541 atcccagcca taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc
    15601 ctccgcccgg acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca
    15661 ataggggcaa accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca
    15721 tacgcgctct cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct
    15781 gatattacgg gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga
    15841 cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg
    15901 acgacgggag ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc
    15961 acccggcggc tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct
    16021 tcagtggcct gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg
    16081 gtgaagcgcg ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc
    16141 agacgcgtgc cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt
    16201 tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg
    16261 tcaaacagcg gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat
    16321 aaggtatcca ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag
    16381 aacagcgccc gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct
    16441 tgcagatccg ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg
    16501 actcgtggta atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat
    16561 gcacgcagag attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg
    16621 accgccagaa tctgtgcccg cgcgccctgg aaaggcactt gtgcttccag accgtgcgcc
    16681 actttactct ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga
    16741 tcgagcatct gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc
    16801 gatagctcta ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca
    16861 accgctgagc gatgcacacc ccacaaatca agatccagtc cggcaaacaa agctactgtg
    16921 gcttccgtat agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt
    16981 aaaaagccgt tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat
    17041 tgtgaaccgg catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct
    17101 tcggcgaggg tgtgtgaacc acttagcgtc cgttggagca gcgcatccag ctgcgggtca
    17161 ttgagttgtt tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcgtcg
    17221 gccagtttga tttgttccgg tttgagctgt tgatgaggtg cagaatcctt acgtaccagg
    17281 gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt
    17341 tgacgattga tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg
    17401 ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg
    17461 tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga
    17521 gtgcggttgc cagaccggca gcgtatttct ccagggcgtc ggcaaactga gctgctctgt
    17581 ccggctgtag tgcgttatcc agcgcctgcg ccgagttcca ggtggcatcc caggcgtgaa
    17641 gaatatcgcg gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt
    17701 cgctgtccag ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg
    17761 ccgcatgcag accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc
    17821 gtgggatccg cattacttta cttaacatac ccagcgtgcc agccagtttt tgcggcagtg
    17881 tgcgcgcttc actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa
    17941 tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt
    18001 tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt
    18061 cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt
    18121 cgacaatatt gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc
    18181 cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttgggt
    18241 tagcgacgat cacgcatgtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt
    18301 aaaaggtgta acagatcaaa caggcgagca gcgtttttac cgcgtagcgc atatagtctg
    18361 gattggtaaa agcatcggcg gccattgatg gcggtttggc tgcgggcgtt ggcggcgtat
    18421 tcgggtccat ctgacccagt tgtaacaacg tctggcagat attctccagg ttacattccc
    18481 gtgccgccat cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag
    18541 caacggcatg ctgcagctta ttgatttctg aggctaattt ttgccgaaat tcaataattg
    18601 cctgagaatc agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta
    18661 ccgttgccac gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga
    18721 gacaaaagac attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta
    18781 gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc aagccgatca ttaagcgcct
    18841 gatgcatttg cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca
    18901 aggttggata aaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat
    18961 agtcgagcat ggcggggaag gtttgcccgt aaatggcgac aatggcgact gcgaaaaaga
    19021 ccagtcccag ccgatgggtg cgcatcaaaa acatacagcc catcaggatc ggcccggcga
    19081 tgaacaatcg gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga
    19141 tctccagcat cgtggcgacc acaaataaga tcgcgacaaa tttggtgtaa aacgcgttcg
    19201 actgaatacc gtaaaacagc actgccagcg ataacgccac gaaagggatc tcaaaggtca
    19261 tcgagatcag aatcaccagc aggcagccta cccagagttg cacggtctgc ggcacgcggc
    19321 ctggtcgccg ctcgcttaac tcttcatgaa agaacgccag cagcctgacc accggtaatg
    19381 gcagggagtt gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat
    19441 gcggaacatt tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac
    19501 gcggacccag ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc
    19561 atccggtagc acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc
    19621 actcatcagg cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg
    19681 gaagttggcg atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc
    19741 agaagcaaat tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg
    19801 aacggtcgcc atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg
    19861 ctgggcaact aatgcatcca cgccgctgac ggcgctggcg gctgactgcg cctgcaacaa
    19921 tacagcatta agatcggctt ctgcagcgcg ctgcgcggtc ctcgcacggt caacgtcttc
    19981 cgctgagaca aaaccttctt tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg
    20041 tttcgcggcg gcacgggctt tttctaccgt ggcgttaacc gagtcggcac caaactgttg
    20101 cgcgtcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc
    20161 agctttcgcc agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg
    20221 tttgactgcc tggttgtcgg tgaccgccag ttctacaatg cgaccgctga cttccggcac
    20281 cacatcaatg gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg
    20341 ccagataacg aaaacaaggg caaccagcgc caacgcaacc actaacagag cagggaattt
    20401 actgcgagga gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa
    20461 tgccagccag aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa
    20521 tgccagattg atatttgccc gctgaataac tcgcctcgtg atgagcatca aaataaggct
    20581 ggcaatggcg cagaaaaacc agctgggata ataagcaccg atcaccggga ttgccggaga
    20641 taatgagcat cccgaaacca tgaagacggg aattatccag gctgtttttt tgagttgcat
    20701 cgccatgcga gagagaactg ttggcatatt aaatacatcc tgtctgctgc gaacaatatc
    20761 tgtagtgaat atcaatatcc attgaaggcg tcagtatggc tattttctcg atgaataaaa
    20821 atagctt
//
LOCUS       DAZFDP010000077        20668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFDP010000077 DAZFDP010000000
VERSION     DAZFDP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..727
                     /gene="mepS"
                     /locus_tag="ACHJ1G_002897"
     CDS             161..727
                     /gene="mepS"
                     /locus_tag="ACHJ1G_002897"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4764722.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            908..2464
                     /gene="pdeN"
                     /locus_tag="ACHJ1G_002898"
     CDS             908..2464
                     /gene="pdeN"
                     /locus_tag="ACHJ1G_002898"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4764723.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSYLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2546..4360
                     /gene="yejA"
                     /locus_tag="ACHJ1G_002899"
     CDS             2546..4360
                     /gene="yejA"
                     /locus_tag="ACHJ1G_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4764724.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            4361..5455
                     /gene="yejB"
                     /locus_tag="ACHJ1G_002900"
     CDS             4361..5455
                     /gene="yejB"
                     /locus_tag="ACHJ1G_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4764725.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5455..6480
                     /gene="yejE"
                     /locus_tag="ACHJ1G_002901"
     CDS             5455..6480
                     /gene="yejE"
                     /locus_tag="ACHJ1G_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4764726.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6482..8071
                     /gene="yejF"
                     /locus_tag="ACHJ1G_002902"
     CDS             6482..8071
                     /gene="yejF"
                     /locus_tag="ACHJ1G_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4764727.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8075..8419)
                     /gene="yejG"
                     /locus_tag="ACHJ1G_002903"
     CDS             complement(8075..8419)
                     /gene="yejG"
                     /locus_tag="ACHJ1G_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4764728.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(8752..9942)
                     /gene="bcr"
                     /locus_tag="ACHJ1G_002904"
     CDS             complement(8752..9942)
                     /gene="bcr"
                     /locus_tag="ACHJ1G_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4764729.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(9970..10665)
                     /gene="rsuA"
                     /locus_tag="ACHJ1G_002905"
     CDS             complement(9970..10665)
                     /gene="rsuA"
                     /locus_tag="ACHJ1G_002905"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4764730.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            10814..12574
                     /gene="radD"
                     /locus_tag="ACHJ1G_002906"
     CDS             10814..12574
                     /gene="radD"
                     /locus_tag="ACHJ1G_002906"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4764731.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            12699..12983
                     /gene="rplY"
                     /locus_tag="ACHJ1G_002907"
     CDS             12699..12983
                     /gene="rplY"
                     /locus_tag="ACHJ1G_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4764732.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13122..14129)
                     /gene="yejK"
                     /locus_tag="ACHJ1G_002908"
     CDS             complement(13122..14129)
                     /gene="yejK"
                     /locus_tag="ACHJ1G_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4764733.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14311..14538
                     /gene="yejL"
                     /locus_tag="ACHJ1G_002909"
     CDS             14311..14538
                     /gene="yejL"
                     /locus_tag="ACHJ1G_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4764734.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14558..16318
                     /gene="yejM"
                     /locus_tag="ACHJ1G_002910"
     CDS             14558..16318
                     /gene="yejM"
                     /locus_tag="ACHJ1G_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4764735.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKQDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16393..16469
                     /locus_tag="ACHJ1G_002911"
     tRNA            16393..16469
                     /locus_tag="ACHJ1G_002911"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16427..16429,aa:Pro,seq:ggg)
     gene            16688..17929
                     /locus_tag="ACHJ1G_002912"
     CDS             16688..17929
                     /locus_tag="ACHJ1G_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4764736.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            18028..18258
                     /locus_tag="ACHJ1G_002913"
     CDS             18028..18258
                     /locus_tag="ACHJ1G_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764737.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            18426..18631
                     /locus_tag="ACHJ1G_002914"
                     /pseudo
     CDS             18426..18631
                     /locus_tag="ACHJ1G_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18646..19122
                     /locus_tag="ACHJ1G_002915"
                     /pseudo
     CDS             18646..19122
                     /locus_tag="ACHJ1G_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            19315..19653
                     /locus_tag="ACHJ1G_002916"
                     /pseudo
     CDS             19315..19653
                     /locus_tag="ACHJ1G_002916"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            19684..19875
                     /locus_tag="ACHJ1G_002917"
     CDS             19684..19875
                     /locus_tag="ACHJ1G_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4764738.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            19865..20086
                     /locus_tag="ACHJ1G_002918"
     CDS             19865..20086
                     /locus_tag="ACHJ1G_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764739.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            20079..20300
                     /locus_tag="ACHJ1G_002919"
     CDS             20079..20300
                     /locus_tag="ACHJ1G_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764740.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            20302..20535
                     /locus_tag="ACHJ1G_002920"
     CDS             20302..20535
                     /locus_tag="ACHJ1G_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764741.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            20541..>20668
                     /locus_tag="ACHJ1G_002921"
     CDS             20541..>20668
                     /locus_tag="ACHJ1G_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4764742.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVT"
BASE COUNT         5168 a         5255 c         5174 g         5071 t
ORIGIN      
        1 tcggcggtag attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt
       61 aaggaattgt ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa
      121 cgatatttgt cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat
      181 tttgagatat atcttgcgcg ggatccccgc gattgcagta gcggttctgc tttctgcatg
      241 tagtgcaaat aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac
      301 atcatcactg caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa
      361 atcgcgaatt atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag
      421 cactaaaaaa ggtatcgatt gttctggttt tgtacaacgt acattccgtg agcaatttgg
      481 cttagaactt ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag
      541 taatttgcgt acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg
      601 tatttatatc ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc
      661 cagcatgaat gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg
      721 cagctaataa accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac
      781 tgcttaggca gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga
      841 tccaggctat agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa
      901 cggaatcatg ttcatacgcg ctcccaattc tggacgtaag ctcctgctta cctgcattgt
      961 tgcaggcgtg atgattgcga tactggtgag ttaccttcaa tttttagtgg cctggcataa
     1021 gcacgaagtc aaatacgaca cactgattat cgacgtacaa aagtatctcg atacctattt
     1081 tgccgacctg aaatccacta ctgaccggct tcagccgctg accttagata cctgccagca
     1141 ggctaacccc gaactgaccg ctcgcgcggc gtttagcatg aatgtccgta cgtttgtgct
     1201 ggtgaaagat aaaaaaacat tctgttcatc tgcgactggt gagatggaca ttccactcaa
     1261 tgaattgatt ccggcgctcg acattaataa aaacgtcgat atggcgatct tacccggcac
     1321 gccgatggtg ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa
     1381 tagcggtgtg tttgccgctc tgaatctcaa cctgacgcct tctctctttt atagttcacg
     1441 gcaggaagat tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc
     1501 ttcacgtttg atgaacgtta atgaattaac cgacatgccg gtccgtgaaa ctaaaattgc
     1561 gggcattcct ctgaccgttc ggctttatgc ggatgactgg acatggaacg atgtgtggta
     1621 cgcattttta ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat
     1681 gagcgtacgt atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta
     1741 cgtggcgtat caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact
     1801 gctacgctgg cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc
     1861 cgaatcgcaa aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga
     1921 tgccgcagaa ttagaaaaag tgctgccagt aggcgtcaaa tttggtatta acattgcgcc
     1981 ggaccatctg cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctacc
     2041 cgcacaccat ttccagattg tgctggaaat taccgagcgc gatatgctga aagagcaaga
     2101 agccacacaa ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt
     2161 cggcaccggg cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat
     2221 tgaccgtgga tttatcaacg ccatcggtac ggaaacgatc acttcacccg tacttgacgc
     2281 ggtgctgacg ctggcgaaac gtctcaatat gctgacagtt gctgaagggg tcgaaacgcc
     2341 ggaacaagcg cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag
     2401 tcgcccgtta ccgctggacg attttgtccg ctggctgaag aaaccgtata cgccgcagtg
     2461 gtaaggtgtg cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg
     2521 atgagtaaaa ggaaatccgt tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct
     2581 gttcaccttt ggtgtgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg
     2641 cgaaccccgg tacgcgttta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa
     2701 aggtgggcag ataacgttgt cagccctcgg caccttcgat actttcaacc gctatgcact
     2761 gcgcggcaac ccgggcgcac gcaccgagca gctgtacgac acgctattta cgacttccga
     2821 tgacgaacca ggcagttatt acccgctgat tgctgaaagc gcacgctatg ctgacgatta
     2881 ttcctgggtg gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac
     2941 tgcccgcgat gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg
     3001 tctggtctac aaaggcacca ccgtcaaagc cattgcgccg ttaaccgtgc gcattgagtt
     3061 agctaaaccc ggcaaagaag atatgttgag tctgttttcg ctgccggtat ttccagaaaa
     3121 gtactggaag gatcacaaac ttagcgaccc gctcgccacg cctccgcttg ccagtggtcc
     3181 gtaccgcatt acgtcctgga aaatggggca aaatattgtc tattcccgcg tgaaagatta
     3241 ctgggcagca aacttaccgg taaaccgcgg acgctggaat ttcgacacca ttcgctacga
     3301 ttattacctc gatgataatg tcgcctttga agcgtttaaa gcaggtgcct ttgatttgcg
     3361 tatggaaaac gacgccaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa
     3421 atacatcatc aaagatgagc aaaagaacga atcagcccag gatacgcgct ggctggcgtt
     3481 taatatccaa cgtccagtat tcagcgatcg ccgggtgcgg gaagcaatca ctctcgcctt
     3541 tgactttgaa tggatgaaca aggcgttgtt ttacaatgcc tggagtcgca cgaacagtta
     3601 ttttcagaat accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct
     3661 ggcaccaatg aaaaaagatc taccgccaga agtcttcaca caaatctacc agccgccggt
     3721 atccaaaggc gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga
     3781 agcgggctgg gtgctgaagg gtcagcaacg cgttaatgcc acaacgggtc agccactcag
     3841 ctttgaatta ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt tccagcacag
     3901 cctgcaacgg ctgggtatca acatggacat tcgcaaggtg gataactcgc aaatcactaa
     3961 ccgcatgcgc agtcgcgact atgacatgat gccgcgcgta tggcgggcga tgccgtggcc
     4021 cagttccgat ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc
     4081 cggcgtgcaa agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa
     4141 taaagaaaaa ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta
     4201 catgctgcca atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca
     4261 gccggccgtg cgccccatct atagcctcgg tatcgatacc tggtggtatg acgtcaataa
     4321 agcggccaaa ctgccgtccg ccagcaaaca gggagagtag atgggcgctt acctgattcg
     4381 ccgtctgttg ctggtgatcc caacattatg ggcgattatc accatcaact ttttcatcgt
     4441 gcaaattgcg cctggcggtc cggtcgacca ggccatcgcc gccattgagt ttggtaatgc
     4501 cggagtatta cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt
     4561 cggcaatatc agcgacagta agtaccgtgg cggacgcgga ttagatccag aagtgatcgc
     4621 tgagatcact catcgctacg gttttgataa gccgatccac gaacgttact tcaaaatgct
     4681 ctgggactac atccgctttg attttggcga tagcctgttt cgcagcgcct cggtgctgac
     4741 gctgattaaa gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat
     4801 ctatctggtg tcgattccgt taggcattcg caaagctgtt tataatggga gccgctttga
     4861 cgtctggagt agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat
     4921 cctgctgatt gtcttcttcg cgggcggcag ctatttcgac ctgttccctc tacgcggcct
     4981 ggtttccgct aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca
     5041 tatcacgctg ccggtgctgg cgacagtgat tggtggcttt gcggcgctga ccatgctgac
     5101 aaaaaactca ttccttgatg aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg
     5161 ggtaagtgaa aaaaatattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat
     5221 tgccggtttt ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt
     5281 gatgttttca ctcaatggtc tgggcttact gggctacgaa gcgaccgtct cgcgcgatta
     5341 tcctgtaatg tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt
     5401 cagtgatatc agctatacgc tggtcgatcc gcgtatagat tttgagggac gttaatgtcg
     5461 cgactcagcc ccgtcaatca ggcccgttgg gcgcgttttc gccataaccg tcgcggctac
     5521 tggtcgttat ggattttcct cgttttgttt ggtttgagtt tgtgttctga acttatcgcc
     5581 aacgataaac cgttgctggt acgttatgac ggcagttggt atttcccgtt gttgaaaaac
     5641 tacagcgaaa gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg
     5701 ctgaaacaac ggctggaaaa taacggctgg gtactgtggg caccgattcg ctttggtgct
     5761 accagtatca actttgctac cgataagccc ttcccttctc caccctcccg gcaaaactgg
     5821 ctgggaacgg atgccaacgg cggcgatgtg ctggcacgta ttctctatgg cacgcggatc
     5881 tcggttctgt ttggcctgat gctgactctc tgttccagcg tgatgggcgt gctggcgggg
     5941 gcgctacaag gctattacgg cggtaaagtc gatctctggg ggcaacgctt tattgaagta
     6001 tggtcgggga tgccgacgct gtttttgatt attttacttt ccagcgtcgt acagcctaac
     6061 ttctggtggc tgctggcaat tactgtcttg tttggctgga tgagtctggt cggcgtggtg
     6121 cgggcggagt ttttacgcac ccgtaatttc gactacattc gtgcggcgca ggcgcttggc
     6181 gtcagcgatc gcagtatcat cctgcgtcat atgttgccta atgccatggt cgcgaccctc
     6241 acctttttac cgtttatttt atgtagttcg ataaccaccc tgacctcact cgatttcctc
     6301 ggcttcggtc tgccgctcgg ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat
     6361 aaccttcagg ctccgtggct tgggatcacc gccttcttgt cggtggcgat attattgtct
     6421 ttgctgatct ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag
     6481 catgacgcaa actctgttag cgattgaaga tttgtcggtg ggttttcgcc atcagcaaac
     6541 cgtacgtaca gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct
     6601 ggtgggtgag tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc
     6661 ttccccgccg gttgaatatc tctccggcga tattcgtttt cgtggcgaat cgctgcttca
     6721 tgccagcgat caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcaaga
     6781 gccgatggtg tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc
     6841 actccaccgc gggatgcgtc gggaagcggc tcgaggcgaa attcttaact gccttgatcg
     6901 cgttggtatc cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg
     6961 cgaacgccag cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc
     7021 cgatgaaccg accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg
     7081 cgaactgcaa ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt
     7141 cagaaaactg gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa
     7201 cgccgctacg ctatttgcct cacccactca tccttacaca caaaagctac tcaacagtga
     7261 accgtcaggc gacccggtgc cgttgccaga acctgcctca acgttgctgg atgttgaaca
     7321 gcttcaggtt gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt
     7381 ggtggtgaaa aacatcagtt ttacgctacg agcgggtgaa acactgggtt tagtgggcga
     7441 gtccggttcc gggaaaagta cgacgggact ggcgctgctg cgactgatta attctcaggg
     7501 cagcatcatc tttgacggtc agccactgca aaatttaaat cgccgccagc tgttacctat
     7561 tcgtcatcgc attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa
     7621 cgttttgcag attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca
     7681 acgcgaacaa caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca
     7741 ccgttatccg gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt
     7801 aattcttaag ccctcgctga tcatacttga tgaaccgaca tcatcactcg acaaaacggt
     7861 acaggcgcaa atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt
     7921 gtttatcagc cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg
     7981 acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg tttgccacac cgcagcagga
     8041 gtatacgcgt cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg
     8101 gttcggcaat tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc
     8161 gatttacaaa caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt
     8221 cgcttaacgc ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa
     8281 gcaatttaag agccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa
     8341 ccttcgaacc tgcgaaacca gcagaccagc gatagttctg cggcagtcga tggacgattg
     8401 agagttgtaa tgaagtcact tattttcccc ggaagcacat tcacttcaca atttgtttac
     8461 atcaatttta acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc
     8521 gcctgcgtgg cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg
     8581 gggctatatg tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact
     8641 ttttacataa attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg
     8701 ttgacatgca tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt
     8761 tttcggtcga ctggcgtaca gacagaagag aatggagctg cttgcgcaga atgcaattga
     8821 ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc
     8881 ccctatgcca aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa
     8941 ctcatcaaga atgaccgcca tcgcattgga tgacaccatc gacacgcagc ccacaaacgc
     9001 cgcaacgcca accaccagcg accagaatcc cagccccagc agcgcactga tgaccatcca
     9061 cgctgccata ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg
     9121 gcgaacgaag cggctgttaa aaatggtcat cacgaacagg aaaacaatgt ttagtgcaaa
     9181 gtaataacca aagttttccg gcgcgacgtg gttaatttca atgtagacaa acggtccggc
     9241 gcttaagaac gagaacatcc cggcaaagct gaaaccactg gcaagcatat aactcaggac
     9301 acgcttatga cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg
     9361 gcgacgctcc ggtggtaagg tttctttaat caggaagaaa atcattgccg aagccagaat
     9421 cgccgctaat gccaggatcc agaagatgta atgccagctc agccacacca gcacccaacc
     9481 gccgacaatc ggtgccatca gcggtgcaat agtggtcacc agcatgacaa acgacatcat
     9541 ccgcgagaac tcttctttcg ggtaaatatc gcgcatcagg gcgttaatga ccacgctggc
     9601 cgcagccgcc gccagcccgt ggaagaaacg catcacaatc agctgatcga tggtgtttgc
     9661 caacgcacac gccaccgcgg cggcggcaaa caccagcgta ccgccgagca ccaccggctt
     9721 acgcccgaag ctgtctgcca tcggcccgta gattaactgc cccaacgcaa agcccagaat
     9781 ataagtactg agggtcatct gcgtactgcc cgcaggtacg ccaaactgcg ctgaaattac
     9841 cggtagcgcg ggcagataca tatcaatcga cagcggcatc aacatggcca gcaggccaag
     9901 gataaaaaca atagcaaacg acgaatgctg tcgggtggtc acaacgggct cctgaaagtc
     9961 atggaaaagt tagacgacgc tggcaatttc ttcttcagtt aacggacgat attcaccggg
    10021 ggctaaatca gcatccagcg taataccgcc aatacgttca cgatgcagct caaccacgtg
    10081 gttacccacg gcggcgaaca tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag
    10141 acgaacctgc gttggggtaa tcacttccag caccgcaggc ttagtgagat ctttttcgtt
    10201 atgcagctgc acgcctttag caaattgctc tgccgtatcg tcagctacag gtgattccag
    10261 tgtcaccaga taggtcttct cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg
    10321 accatcatca gtcatcagca ccagaccggt ggtatcgata tccagacgtc ctgcggcgtg
    10381 cagtttccag gctaccggtt catccagaaa atagagcacc gttgggtgat cagggtcgtc
    10441 cgtggagcaa acatagccct gaggcttatt gagcatgaag taacgtggac catgttgctg
    10501 cgccagcggg ttgccatcgt aagcgacatc atgttcagga agcagtttga acgctgcatt
    10561 acgaacaatt tcgccatcga cggtgacacg attgccgcgg atttcacgcc cggcaatagc
    10621 acggctaacg ccgagttgct gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc
    10681 tgtaaaaacg gaggtcgggc attgacccga aaatctgaac tgttgtctgc ccagtatagc
    10741 ggtctaataa catccctcaa gggaaaaaga tccatggcat actattagca gaataatcta
    10801 cctacgcgag accatgattt ttacactccg cccatatcag caagaagccg tggatgccac
    10861 gctcaaccat tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca ccggcgcagg
    10921 taaaagcctg gtgattgccg aactggcacg gctggcgcgt ggtcgtgttc tggtgctggc
    10981 acacgtcaaa gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc
    11041 cgatattttt gccgctgggt taaagcgcaa agagagccac ggtaaagtgg tatttggcag
    11101 cgtgcagtcg gtcgctcgta atcttgatgc ctttcagggt gaattttcgc tgttgattgt
    11161 cgatgaatgc caccgtattg gtgacgatga agagagccag tatcagcaaa tcctcactca
    11221 cctgactaaa gtgaatcccc acttacgcct gctggggctg actgccacgc cttttcgatt
    11281 gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg atgagaaagc
    11341 ccttttccgt gactgcattt atgagctgcc gctgcgttat atgattaaac acggctatct
    11401 gacgccgcca gaacgactgg atatgccagt agtgcaatac gatttcagcc gcttgcaggc
    11461 acagagtaac gggctgttca gcgaagctga tctcaaccgt gagctgaaaa aacagcaacg
    11521 cattacgcca catatcatca gccagatcat ggagttcgcc gaaaagcgca aaggggtaat
    11581 gattttcgcc gccaccgttg aacacgcaaa agagattgtg ggattactgc ctgccgaaga
    11641 tgctgcactg attactggcg acacccccgg cgctgagcgc gatgtgttaa ttgaagattt
    11701 taaagcccag cgttttcgct atctggtcaa cgtcgcagta ctgaccaccg gatttgacgc
    11761 cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca
    11821 aattgtcggg cgcggtctgc gtctcgctcc gggcaagact gattgtttaa ttcttgatta
    11881 tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga
    11941 caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct tttgggggaa
    12001 aacgaccgcc gacgggacat tgattgaaca ctttggtcgc cgctgtcagg gatggtttga
    12061 agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa attgcccgca
    12121 atgtaacgca gaaaatgata ttgccgctcg ccgctgccgc gaatgtgaca cggtactggt
    12181 tgatccggat gatatgttaa aagcggcgct acgactgaaa gatgcgctgg tattacgctg
    12241 tagcggcatg tctttgcagc atgggcacga cgagaaaggc gaatggttga aaatcaccta
    12301 ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg
    12361 aactgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca tccctctgcg
    12421 ctggatcacc gccgccgata tcctcgccca gcaagcctta ctgcgacatc cggattttgt
    12481 cgtcgcccgc atgaaaggtc agtactggca ggtgcgtgaa aaagtgttcg attacgaagg
    12541 tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga tgtataaggc
    12601 gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt acctgttgct
    12661 gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc aacgcagaag
    12721 tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg
    12781 caatcatcta cggtggcaaa gaagcaccgc tggctatcga gctggatcac gacaaagtca
    12841 tgaacatgca agctaaagct gaattctaca gcgaagttct gaccatcgtt gttgacggta
    12901 aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg aagctgcagc
    12961 acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg ctccggcggg
    13021 gttttttgta tctgcagatt atgcctgatt acggtattgc tattttttgc aggccagata
    13081 aggcgttcac gccgcatctg gcattcagaa cacaaaaccg attaattgcc gccagacgtc
    13141 cggcgctgca attggtcgcg caaattcggc ggcgtgcctt taatggtcaa ggtatcggtt
    13201 gctggatccc agaagatgcg ttcacctaac agcatcgcat caaagttaat agtcaagcca
    13261 ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc
    13321 tcttccagtt cgtagccttt ttcagccgca aactccgtga agctcacttc gcttacgcct
    13381 gccagctctt tcgacagcga ttccagttcg atctcttcgc ctgcctgcag ctgctcatta
    13441 cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg
    13501 gcagtgaaat catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc gttcaatcct
    13561 tcgctggccc cgaggaaatc cataaagaaa tccgccactt tgcgccctac tcgccctttc
    13621 aggaaagtga gatagcgggt ggactctgga ttggtttccc attcggttaa atcgatacgc
    13681 gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc gagattttcg
    13741 ttaacgcgca tactgctcag gttgctcaga actgccacca gcagatactc caccgccaga
    13801 taacggtagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt cgctaattcg
    13861 tcacgcaggc gcccggttgc cgcgcggcta aatgccagaa agtcctcttc gccctgacgc
    13921 tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta agctttattt
    13981 ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc tgtcggttcc
    14041 agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat aagctggtgc
    14101 agggcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc gggcggtatt
    14161 caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag aaggcagcgt
    14221 aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt
    14281 gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta cagtgatgaa
    14341 caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa ggctccgacc
    14401 gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac cagcattgcc
    14461 ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc ctctatcaac
    14521 gaagacaaag cgcactaagg gaaacagata actggttatg gtaactcatc gtcagcgcta
    14581 ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt tcaatattct
    14641 gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga caacgcttgc
    14701 tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg tgttcgccac
    14761 ctacttgctg atcctcttcc cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt
    14821 tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgata gcgaagtctt
    14881 tactcgtttc catctccatc ttaatcccat cgtctggcaa ctggttatca acccagacga
    14941 aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta ttttattgct
    15001 tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc gtcgtcgacg
    15061 cttcgcgcgc ccgctggccg cattcttatt tatcgccttt atcgcctcgc atgtggtgta
    15121 tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta acctgccgct
    15181 ttcgtacccg atgacggcgc gacgttttct tgagaagcat ggtctgcttg atgcgcagga
    15241 gtatcaacgc cgtcttattg agcaaggtaa tccagacgcc gtttccgtac agtatccgtt
    15301 aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtgctgttga ttactgtcga
    15361 tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca
    15421 aaatatttcg ttcacgcgcc atatgagctc cggcaacact acagacaacg gcatctttgg
    15481 cctgttctat ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc
    15541 ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct cgtcagatgg
    15601 ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg
    15661 cacccaatcc gacgagcaga ccgccacgca gtggatcaac tggctgggcc gctacgcaca
    15721 agaagataac cgctggttct cgtgggtctc tttcaatggc actaacattg acgacagcaa
    15781 tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg accagatcaa
    15841 ccgcgtgctc aatgcactgc gtgattctgg caaacaggac aatacggttg tgattatcac
    15901 tgccggtcgg ggtattccgc tgagcgaaga cgaagaaacc tttgactggt cccacggtca
    15961 tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct
    16021 gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg tcagcacacc
    16081 tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc attactgggt
    16141 tactgcagcg gataacgata cgctggcaat taccaccccg aaaaagacgc tggtgctgaa
    16201 caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag atgaaaaacc
    16261 acagttaagt ttgttattgc aagtgctgac agacgagaag cgttttatcg ctaactgatt
    16321 aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta
    16381 gcagccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt
    16441 cggaggttca aatcctctcg tgccgaccaa atcgcccacc ggatatcacc ggaaagcacc
    16501 ggataagaca gggataaccc gctaatcact tgattggcgg gtttttttat gttttttagt
    16561 caccgaacat caccggattt ataagctatc agccggattt tttagttata cgtttagtta
    16621 tacgggctgg cgtataacta aagccgtata actaaaaccc tgttttttcg tgtggaatca
    16681 tagacatatg gcgagaaaaa cacacccatt aaccacagtg cagatcaaag cagccagacc
    16741 agcgcaaaag gagtacaccc tgcaggacgg cggagggctt tttctcctgg tcaaaccgtc
    16801 tggatcaaaa ctctggagat tttcctacta ccgaccatcg gacaaaaaaa gaatattgct
    16861 gagttttgga tcgcttgaag atgtttccct ggctgatgcc agaaaacgcc gtagcgagta
    16921 caggacgtta atcagtgccg gaactgaccc gcaggaccac gagaggcaaa aaagagagac
    16981 agaggcccga agacaaggga acacgttcga aaatgtggcg gcggcatggt accaggtgaa
    17041 aatcagccag aatctggccc ccaacacgat taaagacatc tggcgttcgc tggataaata
    17101 tgtattcccg ttcatcggca acacgccaat agataccctc accgcccgaa ggttcgttga
    17161 agtgcttacc cccatcaagg agcgcggcaa cctggaaaca ctcaagcggg ttttacagcg
    17221 cgttaatgag gtaatggatt acgccgccaa cagtgggctg attgatgcca atccggctat
    17281 gaatgtgcgt aaggcgtttc cctcacctgt aaaaaaacat atgccaacaa tccgccccga
    17341 acagctgccc gagcttatgc aggctttatc agtatcggca acagaacggc agaccagatt
    17401 actgattgaa tggcagttac tgaccgtaac ccgtcccgcc gaagcgtcat caacgcggtg
    17461 ggatgaaatc aacctggacg cgaagcaatg gacgatacct gccggacgca tgaagatgcg
    17521 cagggatcac gttatcccgc tttccggtca ggctatggcg gtgctggagg ccatgaaacc
    17581 aatcagccac caccgcaatt acgttttccc aagtctgaaa gacccacagc agccgatgaa
    17641 cagccagaca gctaacgcag cattgcggcg tatgggattc gctggcgtgc tggtgtctca
    17701 tggattacgc gccatattca gcacagcagc gaacgaggaa ggattcgagc cggacgtaat
    17761 agaggcggca cttgcccacg tcgacaccaa cgaagttaga cgggcataca accggagcaa
    17821 ctacatagaa aaacgcatcg tgctgatgcg ctggtggggc gaatttgtcg aggctgcggc
    17881 gacgggcgta accctcgcca gtggtaaaag gggtatccga gccgtgtagc tgtacagaaa
    17941 accagtaaaa actacgaaaa ccatgtaaaa ccgtcgtata attgcatcaa atttaacgac
    18001 aaggccgtga aacatgaaac cgttaagatg caaaaaaata tcagatgcaa ttgcgacggg
    18061 ctgcaactgg ccctgatggt tcagcatgaa ttttggtcaa cctacgatcc ggaggacaga
    18121 acgacggccc catcaaaaaa acaggtagta gattttctgg tatcccgtgg cgcttccaga
    18181 aatctggcgg taagtattga taaggtcgta cgtccggcat ctatgaagat cggaggcagg
    18241 cccaaaaaat ggcggtaaca atcctggaag cggcagaaat gccgcttttt ttataattcc
    18301 atttcaaatc atcaatataa aaaacggtgt ataccgttta aaaacggtgg gaactgtttt
    18361 tacccatatc cgatgattta ccgtatttgt caccggaata caccggattc acaaggtaaa
    18421 tcacgatgga agcaatcaga aaaattatct ttcgccagga agtaaaaaaa ttatccacat
    18481 caaggcagac agcacgctgc aaagcatgat caacgccgga gaatttccgc agggttttcg
    18541 cgttggttta cgccgtcgcg gatggtatga ggatgatgtg ttgtcctggc agaaagaacg
    18601 cgaagaggaa gcacgcggaa cggctgctta acgggtatca cagatatgac aaacacgaaa
    18661 aaagcggccc cgatatggag ccgcctttct gaacaattaa cccgctgcgc cttatgtgta
    18721 tgtgatccca aacataagca cggggatgat agccgctatc aggctggtgg gcaatgcaat
    18781 cagtctggtt cagttcgttg ccatacctgc aatgagcgct tttccctgta ctctttaagg
    18841 aattgctcaa gggcaaaagc acatggcgcg aatctttctg attcatgctc tatctttctg
    18901 cgccgtcttt tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt cattgtgttg
    18961 tcctgcatag caatgcgccg taatacctca caccacggcg ctgatagttt ttatcctttg
    19021 ggttctatgc cgcgttgctg taattctttg cggagaacgc gtttaatcca agtggctaaa
    19081 gatgtgtcgc catcggcctt agctgcttct tctagctgcg ctctgaagtc ttccggtaat
    19141 cgcatttggt atggaggcga acgtttttct aatggtgttg acatggtaat tacccttcat
    19201 tactatacct acatggtaat taccattgta cttgccatta cacaaaaatc aacgccccgc
    19261 agtgctggga acacatacgg ggcgtctaac caacaacgta aactaggagc cgttatggtt
    19321 gccgtaaatc atataccaca ccttgtacac acacaaacgg cctttgtgtg gcgttttctg
    19381 gccctgaata tcggagaaaa aaaccagttg atcgccagtg tcgaaaatag ctatgattcc
    19441 cgcgcacctc ataaaacggg tgccgggatt ggcgtcctgg gcaaaactga agcgcataac
    19501 acgcgccccg cgtgtttttt tgtgccgcat agtcacacct tatcaatggt gggctgtacg
    19561 ggggcggagc aatccgcgcc ggtttcttca gtgtccggtt acgccaaccc tgtacagtcc
    19621 gccaccagcg aaattggcgt ttccggtggc ggttatctta accacactga ggaggctgcc
    19681 aacatggcta ctatccctac ccttgtacat tctcaaaccg cctttctctg gcgctttatc
    19741 atctttggcg cgtcagaaca tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg
    19801 cgtagccgtt gcccgtccgg ttgtgttgct gtattcgccg cccgtattcg tcaggaggtg
    19861 tgccatgtgc aataaaacca caccggacgc agcagccgcc gcgctcacta cgctgatgca
    19921 cgcgcttatc gatattgaat gcactgcaga gcttgcgcag ggagaagaac agaaagaccg
    19981 gacacagttc gccctggaat gtatccgata catcgcaacg cggtcgctga atgacgctaa
    20041 aaatattctt gttgctgatt gtgaaaatgg ggggggttat gcgtgatgat cgttttaatt
    20101 ccctgaaaca agaattttcc ggcgttcctg atgatgcggc tgatgcgctt tcgtcaatat
    20161 ctgaaattat gcgggtggct tttttctttc tttgcactga tgagcacaga gatacagggc
    20221 taaatattct tgatattgcc gctaactatg ctgatttcgt gacagaagct gttttaagaa
    20281 aaacaacgga cggggattaa tatgcgtgat atttaccacg aaacaataga ccgcgcattt
    20341 agtgcccttg cttacgctga aggtatgtac gaaatattgc gcatatggct tgaaacactt
    20401 ggcgacaatg aacgcgacaa acaaaaatca agaattgtca cggcattaat aacgcttctt
    20461 gagcctgtaa taaatgagtt gcaggaaata gagacattgc acgacagata taacgaacag
    20521 cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca
    20581 ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc acggtaatga
    20641 ttaagggcgt ggcgggacgg tgcgtaac
//
LOCUS       DAZFDP010000078        20539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFDP010000078 DAZFDP010000000
VERSION     DAZFDP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..253)
                     /locus_tag="ACHJ1G_002922"
     CDS             complement(<1..253)
                     /locus_tag="ACHJ1G_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764743.1"
                     /translation="MASGFLGRYHHQIIVCIYFNGQFLNRRKVANHDENPEKNAHPKM
                     GKLDARNALRRCHLGAWSTWQLGGLSGRTRPSHAYRRACR"
     gene            610..684
                     /locus_tag="ACHJ1G_002923"
     tRNA            610..684
                     /locus_tag="ACHJ1G_002923"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:642..644,aa:Asn,seq:gtt)
     gene            690..776
                     /locus_tag="ACHJ1G_002924"
     tRNA            690..776
                     /locus_tag="ACHJ1G_002924"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:724..726,aa:Tyr,seq:gta)
     gene            781..856
                     /locus_tag="ACHJ1G_002925"
     tRNA            781..856
                     /locus_tag="ACHJ1G_002925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Thr,seq:tgt)
     gene            1947..2774
                     /locus_tag="ACHJ1G_002926"
     CDS             1947..2774
                     /locus_tag="ACHJ1G_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4764744.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMDLEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            2802..2877
                     /locus_tag="ACHJ1G_002927"
     tRNA            2802..2877
                     /locus_tag="ACHJ1G_002927"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2835..2837,aa:Ile,seq:cat)
     gene            3158..4522
                     /gene="dnaB"
                     /locus_tag="ACHJ1G_002928"
     CDS             3158..4522
                     /gene="dnaB"
                     /locus_tag="ACHJ1G_002928"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4764745.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            4522..5520
                     /locus_tag="ACHJ1G_002929"
     CDS             4522..5520
                     /locus_tag="ACHJ1G_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764746.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQFHRPYSPGVESPEDYVEAVLTAATSCSAKAILAPGLRYFTTVPREIGVQLRRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQDLFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLITIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(5567..6199)
                     /locus_tag="ACHJ1G_002930"
     CDS             complement(5567..6199)
                     /locus_tag="ACHJ1G_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO4764747.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(6192..7208)
                     /locus_tag="ACHJ1G_002931"
     CDS             complement(6192..7208)
                     /locus_tag="ACHJ1G_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4764748.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            complement(7210..7995)
                     /locus_tag="ACHJ1G_002932"
     CDS             complement(7210..7995)
                     /locus_tag="ACHJ1G_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HFO4764749.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRTNQSFREFLKRTPRDRITLGTFSDVKKWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            complement(7982..8710)
                     /locus_tag="ACHJ1G_002933"
     CDS             complement(7982..8710)
                     /locus_tag="ACHJ1G_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4764750.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEDD
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRNKNAPETAPEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            complement(8714..9931)
                     /locus_tag="ACHJ1G_002934"
     CDS             complement(8714..9931)
                     /locus_tag="ACHJ1G_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4764751.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADICKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            complement(9941..10201)
                     /locus_tag="ACHJ1G_002935"
     CDS             complement(9941..10201)
                     /locus_tag="ACHJ1G_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764752.1"
                     /translation="MCESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAG
                     LEDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            10465..10710
                     /locus_tag="ACHJ1G_002936"
     CDS             10465..10710
                     /locus_tag="ACHJ1G_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764753.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            10713..11291
                     /locus_tag="ACHJ1G_002937"
     CDS             10713..11291
                     /locus_tag="ACHJ1G_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO4764754.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLYAA"
     gene            11358..11513
                     /locus_tag="ACHJ1G_002938"
     CDS             11358..11513
                     /locus_tag="ACHJ1G_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764755.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            11617..12117
                     /locus_tag="ACHJ1G_002939"
     CDS             11617..12117
                     /locus_tag="ACHJ1G_002939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764756.1"
                     /translation="MFDDIYALYAGDLSWWKQYGSTIPGGKFRKVTANLAAAKSFSLE
                     YRRYCGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQV
                     SISKWQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLR
                     RAECRL"
     gene            12015..12641
                     /locus_tag="ACHJ1G_002940"
     CDS             12015..12641
                     /locus_tag="ACHJ1G_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764757.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNMNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            12638..13315
                     /locus_tag="ACHJ1G_002941"
     CDS             12638..13315
                     /locus_tag="ACHJ1G_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO4764758.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            13312..14013
                     /locus_tag="ACHJ1G_002942"
     CDS             13312..14013
                     /locus_tag="ACHJ1G_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764759.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            14315..15577
                     /locus_tag="ACHJ1G_002943"
     CDS             14315..15577
                     /locus_tag="ACHJ1G_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764760.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLAEAELPFEPEFIDELANKGLHSLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            15650..16156
                     /locus_tag="ACHJ1G_002944"
     CDS             15650..16156
                     /locus_tag="ACHJ1G_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HFO4764761.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLHQQ"
     gene            16351..17076
                     /locus_tag="ACHJ1G_002945"
     CDS             16351..17076
                     /locus_tag="ACHJ1G_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764762.1"
                     /translation="MKGEVKKRGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVRKIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNDISECDARDEGVPPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWSANPWVWVIEFERIQ"
     gene            17116..17307
                     /locus_tag="ACHJ1G_002946"
     CDS             17116..17307
                     /locus_tag="ACHJ1G_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4764763.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKVEKAEGATK"
     gene            17304..17519
                     /locus_tag="ACHJ1G_002947"
     CDS             17304..17519
                     /locus_tag="ACHJ1G_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764764.1"
                     /translation="MSKPTDEEIVRVLEEHGRCMTYVVTNWLRDKYRTLKTAYVLRRL
                     KKLEFDGKVKRVNSSYIRQICWEASSE"
     gene            17512..18156
                     /locus_tag="ACHJ1G_002948"
     CDS             17512..18156
                     /locus_tag="ACHJ1G_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764765.1"
                     /translation="MSNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKEQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            18153..18746
                     /locus_tag="ACHJ1G_002949"
     CDS             18153..18746
                     /locus_tag="ACHJ1G_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4764766.1"
                     /translation="MTIDYQALRDAAVAVETEPMHQNFVAFRMAFTPSVALALLDEIK
                     RLEDTNIDAMCRIAELETNLAALVAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIIND
                     ALHHSETPATDAFLAEIRAEARNEGINYTASRLAAAFNHGFINKSLREVFDVTRMILS
                     AKEELANEAHPIDGLSGEYAEKSLEEWAEQIRKGSSQ"
     gene            18743..19033
                     /locus_tag="ACHJ1G_002950"
     CDS             18743..19033
                     /locus_tag="ACHJ1G_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO4764767.1"
                     /translation="MNIDTTITIDTLLNTGLALLGWLFIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
     gene            19030..19734
                     /locus_tag="ACHJ1G_002951"
     CDS             19030..19734
                     /locus_tag="ACHJ1G_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764768.1"
                     /translation="MKFSKFSELVNRILFNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGTVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSIELDAAKQRIAELEGNCAALAAENAAMHETIEAVRGVADNSSGIAGWHLNGDIATW
                     EEILPEINDIETPATDAFLAEVRAQGVEMLAKNHQSIVNALKGDSLFSDGEYRHAAIV
                     SAAVYFAAELRKGGNQ"
     gene            19731..20387
                     /locus_tag="ACHJ1G_002952"
     CDS             19731..20387
                     /locus_tag="ACHJ1G_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4764769.1"
                     /translation="MSEINYQALREAAERATPAMERLLMLPVDDDLLSEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKSRDQENEEIALTEGKLLIENGRLVADT
                     LRHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIA
                     ELEAREISLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGEEHGNKTRR
                     "
BASE COUNT         5368 a         4634 c         5351 g         5186 t
ORIGIN      
        1 cgcgacaggc gcggcggtaa gcatggctgg gcctagtcct cccagacaaa ccaccgagtt
       61 gccaggttga ccatgcgcct aagtggcaac gccgaagtgc gttacgagcg tccagtttgc
      121 ccatcttcgg gtgggcgttt ttttcagggt tttcgtcatg gttagcgact ttgcggcggt
      181 ttagaaactg accattaaag taaatgcaaa cgatgatctg atgatggtag cggcctaaga
      241 agccagatgc catggggtat gagtcgtccc ccgtcaaaaa atcgaccgca gagtgtcccc
      301 gtctgtgtat tagggaacgg ggaggcacaa caggtaaggg cgctggtgtg attaaccaga
      361 tgaacgagaa ggggccatct gttggtcagc gtcctttcct gttgcgtttt cttttcagcg
      421 taacagcggt gcttaacagc actttgggta cagttccacg aatttacggg tatatcccgt
      481 catgctgaag gcgctaatca cgctggaagc cagggttatg catcccctgt taccgaattg
      541 cagccagggc gcggtgcgcc gaaaagcata cggaggtgga agccctcgcc ggagacgtac
      601 ccggcaagtg atggtgtagc tcagcggtag agcagttggc tgttaaccaa ctggtcggta
      661 gttcgaatcc acccaccatc gccactttag gggagttagt ccgtaggggt agcggggtag
      721 actgtaaatc tactgtcatt gcgactcggg tggttcgact ccatcactcc ccaccacatt
      781 gccgatttag ctcagttggt agagcagtcg ctttgtaagc gaatggtcag cggttcgatc
      841 ccgttaatcg gcaccaacat aacaggtaag agcattctcc cttatggggc ctggcttaaa
      901 tgcatcgagt gctcttaccg ttgtgatgaa gtgcagatct tagaagcaac cagaagataa
      961 gcatctggct tcacaacata aaccgcagga acgaccaata aacggtagtc cgtatggaga
     1021 acaccccgtt gaggaagagg cctggccgga accgtaaccg gcgactggta atgtgtgtaa
     1081 atggagcgaa atatgtgggc gtttaacggg atacgggttg taacagcgat tattaacggc
     1141 gaacttaagt attcacgttt cgagacaaat gaacgtgatg ggaaatgtat tggtgtgcgg
     1201 ttagtaagag aatacgcata acaactacaa cgttgagaac actggcgtaa cggggtcata
     1261 tcccaatcta cgaataaatg ttgcgttgcc gcgtgacaac cagtgttctc aacattgtgg
     1321 tgaatgcaca ggctgatgtg ccgcaactac agtagtgcgc gctttgcggg gcttgctaca
     1381 accctgtgtc ggagttcagc accgaccatc acagtttgat tctctggcat gagcataacg
     1441 ctgaaataag tccagtctgg tgcggtctga tcacccgccg ttagctccac gaaacggagc
     1501 acgtaacagg taagagcatt ctcctgtaac gggttcatat cccaatctac aggtccacca
     1561 agaatgctct ttccgttgcg gtgaatgcgg ctaagcgcac gcggggaaat ggttatatct
     1621 gtccattatt tctccttgtt tccacgtcca cggtggataa ccagccaaag gacaccggga
     1681 ggaacccggc accgcagctt ttttattcgt taaataatgg agtgagagga tgcagaacaa
     1741 tccgaacaaa tgtcgaacgc tatgggtgcg gttatatatt tatgccgtcc tctgtttgat
     1801 tgtgtcactg gttctgtatg tttggctttt gccaaatatg atctcatcta acagcacaat
     1861 acttgtattg ttgggagtcc ttctcgcgct catttacccg gctttcgcag tagtcttttt
     1921 tcgtgaaaaa accaggaaat taattaatga aaaaaacgtt gattagtgca gcgattattt
     1981 tgggttcttt atgtctgacc ggatgcgatc gggtagagcc aggtaacgta gggatcaaag
     2041 taaataagct gggggatgat aaaggtatcg gtgaagtagt tggcgttggt cgctactgga
     2101 caggcttgaa tactgaagtt tatatcttcc cgacctttaa gcaaatgaag acatacgatg
     2161 agccgttcag cttccagatg agcgacggaa caaccattgg ttaccacatc ggcgtagcct
     2221 acaaggttga tccagcaaaa gtcacaacgg tatttcagac ctatcgcaaa ggtgtagatg
     2281 atattactga taccgatcta cgccagaagg ttgcagatgc tctgaaccga ttagccagca
     2341 aaatgaccac cgacaaattt atcgacggtg gcaaatctga attgcttgat gcagccctta
     2401 aagacattca ggaagaaatg acgccaatcg gcattcaggt gatgagcctc tcttatgtag
     2461 gtaagccaga atacccgcca acagttattg acagtattaa cgccaaagtc acggcaaacc
     2521 aaaaaaccct gcaacgcgag caagaggtca aacaacgtga agcagaggcc aacatgctgc
     2581 gcgcggaagc tgccggacag gctgatgcta ttcgcacaaa agcccaggcc gaagctgatg
     2641 ccattcgttt acgtggtgaa gctctgcgcc agaaccccgg tgttatggac ttggaagcga
     2701 tcaacaaatg gaacggtaca ttaccgcagt atatgaccag taataccgct gttccgtttg
     2761 ttccggtgaa ataaaagcgt aagcaaaatt ggcagtaatc cggcccttta gctcagtggt
     2821 tagagctggc gactcataat cgcacggtca ccggttcaag tccggtaggg gccaccatat
     2881 ttggttgtaa cacggcgtct ggcacatgcg tcgttagcgg tctggtgacg ttaaaggggt
     2941 aacctttccc ctagctcagg caacaaacca ggtagccgga atgtgcaagc cccgttcata
     3001 gcgtcggact gcggattcac catcctggcg attcggtgtg acagccggga agagtccggc
     3061 gcattaatcc tgattttctg gtgatgactc atatcgttag gagtgatttg agtatgccga
     3121 ttatatctga cattcagcac gcctgggtgg agtgctaatg tctgcatccc ctcttgaatc
     3181 catgccaaat tcccttagtg cagaacaagc tgtacttggt ggcttaatgc ttgataactg
     3241 ccgctgggat gaagttgcag atcgtatagt tgctgatgat ttttatacca gtgctcatcg
     3301 tgaaattttc agtgagatgg agaggttatt aagtcatggc aaaccgattg atttgataac
     3361 acttgctgaa gcacttgaac agaacggtaa attagaacgc gccggtggtt ttgcgtacct
     3421 tgcggagatg tcaaagaaca cgcccagcgc ggcaaatatt tgtgcttatg cggatatcgt
     3481 tcgtgaacgc gcggttgttc gtgaaatgat ttccgtcgca aatgaaatag ctgaagctgg
     3541 atatgcgcag gatggcaggg gcagcaatga attgctggat atggccgagc gccgcgtttt
     3601 tgaaatagct gaaaaacgac aaaagagcgg tagtggtcca aaagatatcg ccagcattct
     3661 cgatgcaacg gtatctcgca tagaagagtt gtttcagcga ccgcatgatg gtgtaacggg
     3721 gcttgatacc ggatttaccg atctcaataa gaagacggca ggacttcagg cgtccgatct
     3781 cattattgtc gccgcccgcc catcgatggg gaagactacg tttgcgatga atctcgtcga
     3841 aaatgccgca gtccgtaacg ataagcccgt attggttttt agccttgaga tgccgagcca
     3901 ccaactgatg atgcgctcac tggcttctct tgcacgcgtt gatcagactc gtattcgaac
     3961 agggcaactt aacgacgagg attgggcgcg ggtttctggc gcaatgggga ttctgttgga
     4021 caagcagaat atttttattg atgactcaag cgccctaaca cctacagagc ttcgttcccg
     4081 cgctcgtcgt gtttataaag aaaatggtgg tttgagcatg attatgatcg actacctgca
     4141 acttatgcgc gtccccgagc tgcaagataa ccgaacgctg gaaattgccg agatttctcg
     4201 ctcactgaag gctttggcga aggaattaca agtaccggtg gtggcattgt cacaacttaa
     4261 tcgatcgctt gaacagcgtg cggacaaacg accggtaaat tcagatttac gtgaatcagg
     4321 agcaattgag caggacgcag acctgatcat gtttctgtat cgcgacgaag tttatcaccc
     4381 ggatagcgaa atgaagggca ttgccgaggt aattatcgga aagcaacgaa atggcccaat
     4441 tggcacggtg agattggctt ttaacggcca atactcacgg tttgataact atgctggtgc
     4501 tgactggcaa gaggattatt aatgcaatgg aatgaggaaa agccgatgaa catcctgatc
     4561 attgggcgaa aatttgaagc tatcagtgat gtgaaaacat atacggaaat gtgggcttac
     4621 aacctggcct gcgcctttag tgaggcaggg gtaacattgc aattccatcg tccatattcc
     4681 cccggcgtcg aaagcccgga ggattatgtt gaagctgtgt tgaccgctgc gacctcgtgt
     4741 tctgcgaaag ccattttagc gccaggattg cggtatttta ctacggtgcc cagggaaata
     4801 ggcgtgcaac tgcgtcgtcg attcactgga tgggtagccc aggtatacga cggttctatg
     4861 ctggattcgg caccagtcga tattactttt actgtccgcg atgatacctg gcggtacctg
     4921 gataatcccg gcaggttaga gcgtcataat cgctttaaca aacatgttgg atgggcagcg
     4981 aatcaggatc tgttccatct ggaaaccaaa acagacgatg ttctgcgtat ttttgtagac
     5041 cacgctgcat ttgatgttag tgggtttgat cactccttaa gtatccttat gaaccttcag
     5101 cgtctgaccg ttccgtatga ggccagaacg ttgaccgatg acggattgat taccattgat
     5161 ccggggaata tatcggtaac cccatacaga cggacgccgg taccagcaac cgaatttgca
     5221 gctgaattgc gtaagagtga cgtgtttatc gttacgcatc ccgaaagcct tggattaact
     5281 gtacttgagg cggcaatgtg cggggcgttg gtattaacgc ctcccgattg ccttccgcca
     5341 gatcgcctgg ctttggtgaa ccatatggtt atcaagtcgc ggattgattg ggatgaggtt
     5401 attgctcgcg ttgatcgcgt gaaaaatgct gaaaaggtcc agtgtcacac ctggtcggca
     5461 attgcggaaa agatgcttga gacgtttatc acgcagaaac cgtcgcgcgg taacggataa
     5521 aaaattgaac ccgtcataac agaaaagccc gaacgccggg cttttcttaa gccttgtcaa
     5581 cagagacttg agcggctttt atggatagat tcccgctggc ctctatcgcc atacttcccc
     5641 ccgccttcag ggcgacatcc gcgcctgact ttatatcgag atttcctgcg gaagagatga
     5701 atgccggacc ttgagaaatg gcatataact ccccggcctc gttgaaccct attgttgttc
     5761 cacttttcaa gtgcgtaacg gcccaggctc cgcccgccgt ccggacctcc attagtccgt
     5821 tccgcgacga aataaagtct tttttggcgc tggttgatgg ttgtgctggt gcaccttcga
     5881 cttcaggcgg tacatagcct tcaccttgtc ctgacgcttc aggcggcaca ttgggagcgc
     5941 caccggatgc atcctgtgca taaccgatta tcaatggcca tcgagaatcc ccattgtagg
     6001 gaaattctac ccatacttta tcgccgggca gaaatggtga aaacgtgttt gcattggaca
     6061 atatagcttc tgcccacggc aatgaggcat ctggtaaccc atccatcatg ccgacaacgc
     6121 gtatttgtgt acgcatcaga cctttagggt catcgacgct taccactaca gcccgatact
     6181 tccctgtcaa actacccatt caccactcct aactgtgctc ggctgacaaa acggaagcgg
     6241 tcttcgaaat gagtcacgga catcactatc attttgtcag ggatagattc atcgagttct
     6301 ccgtcacctg ccgtgttatg cacgacaatt ttcagcgtcg tacccggagt tagcgcggca
     6361 tttccttcca ccagcatatc gaggcggggg agaatgaatt tgttgtagtt cgccagcgcg
     6421 gtaggatcgg gattgctcgt aaatttaatg gggtcttcct ggttacctga gtaaaccaca
     6481 cctttggtca tgtcataact ggccattctg taattgtggc ggcgctggta ttcataatcg
     6541 gcattcagga tgttgaactg actaattgta aatccggatg tgttgggatt ggcggactca
     6601 taagtaagcg atggagcggc gtttgccatt ttttccatac ttttaaaatt gatcgtcccc
     6661 ctggatgccc agcacataga accggtatcg cgggctatct cctgcaatac cttggtcggt
     6721 ttttctccaa catttaggtg atatgtggat gtttttctga atgagtcagc atttaccttc
     6781 agaccagggg caagagagga aactacggct gatgggggct tatcaacaaa atactgtgcg
     6841 ctggtggacg gaacttttaa taaccgcacc gggttactaa acgcgtaaat cagtacagta
     6901 tcgtccttgc gcggcgcttt aagaacgaag aactcttccg agaacaggat gccgccatga
     6961 ccttccggat caccaagtga aacagtcagt attgttccaa atttcacccc cagcttattg
     7021 accacgtaag ccgttgaatc cctgaccatg agcataagct ggggaccaga tagctccccg
     7081 ggttcgacat aggtacatcc tacgatcatt tcgcgaggga tttcgttctg cccaattgaa
     7141 acagattgca ggaatagctg agtgcgtttt gaatcagttt ccggggctgt ggtggtcttt
     7201 gtggccatct cattcctcca gaattttcgc ttttaccgtt atggtgccgg tggtttgctg
     7261 catataagcc aggataggaa gctccgccac aacggtgagg ttcaatccaa ccgcgaacag
     7321 cctgttgtcg gcggtgccgg tggtcagatc ctgaaatgcg attgattttt gcccttctat
     7381 gtaacaggta accggtatct cataaccgcc gacattggca atgtgagtga aagatgcctg
     7441 cccgaggctg gcatacattc gtagccagaa tgctaatgca gttgtaacca tcccaagaga
     7501 ttccttctcg tcactggcta tccatagcga atattccagt gagaaaggga tagtcgatac
     7561 cagggcttca atctcatcat tttcattggt gacatgccct tcatcgtaat tatcccggca
     7621 cagttcacct tcataaattg aaaacgcggg agaacgagac agattcacaa gcggcattgc
     7681 cagcttattt accgggccag cagaggcagt atctttacgc ccggcgcgat cggcttcaaa
     7741 tgacgacaac cacttcttca catcactaaa agtgccgagc gttatgcgat ctcttggggt
     7801 gcgtttcagg aactccctga atgactggtt agtgcgatca ttaaagctga caacttgtga
     7861 gtcgaacgct tcgtttaaag cctgtgcgag cgccgaatca atgccatcaa tagtggcaaa
     7921 ttccagctta ccggttggag taagaccttt tttcttaaag atggccagta gccattcctg
     7981 attattcaga atcaccgatg aaattccctt caaaggcgcg tgaaggcacg caataaaaca
     8041 aactgcctac cctggcagtg ccgtaattga atattttatg gatgtaccag aagcggcgaa
     8101 tggttgtgcc gtctgacagc tgttccagcc attcgagcat agaacccact ggcacattga
     8161 cggcggctaa ccgaaggatt aaagcactgt cgctaattcc cgtattatca ctgccgtcgt
     8221 atagcgcgta gaaggcgtcc atctcatccg ggcagtcgag ggccgttatc agttctggat
     8281 cctgatagtc atatatgcgt tggttcggtt ctattatttc aggtgccgtt tcaggtgcat
     8341 ttttgtttct gtaaggtatt gcgcgataca gaactgcatc gaatgagtca gggtctagct
     8401 tgattgcttt gagccagtcc atccgcacaa ggttattaaa aactgcatga ccttgataac
     8461 ggtggcgcac accagaatca ctaagcaggc cgtgatccag attgggaagg tgattgtcct
     8521 ccacaggatc aacaatatta ccaacgttaa caccatcggt ttcgatttca gcatcaatat
     8581 catcctcttc aatcagttca gaaccttcgc ctggaatatc cggatccgat tcggtgtccg
     8641 ggaggttatc accagtcact tgttgtgatg gttctgtgtc ctcaaacatg tcatcaaaga
     8701 aaccagccat cgattatcct ttccgtttac gggcttcatt aatttgtgtc tcaagaatgc
     8761 ttcgcgcctg cgcggtggca gcggccttgt ccattccctg actcatgaaa aactttatga
     8821 ggttgttcgc ctgcgtttgc agggcttttt tgagagcgtc ggcttcagcg cgagcctggg
     8881 cttccctcac ccgcgatgct tttagttcgg cattcttcct gtttgccgtg gtgcgagctt
     8941 tttttaacaa ccggcgaacg ttgtccgtgg cgctatcttt ggcgcgtagt tttttgccta
     9001 atgcatcctg agatttcaga tacagctcat actcacgcgc agctttagcc tgatccgtcg
     9061 ttgttgtccg gttgcgcgcg agcgatttag ccagttcgcc tttgaaatag gttgttgtct
     9121 tccgcttgtc atcgccgaag gctacctgtt cagctgcttt ttccagggca ataatgatgg
     9181 ccttgtgcca tgtgggagac tgaaaacgcg tcatagcgtg caacacatgt ttgcaggcta
     9241 caccagtcag atcagggttg cggatcttgg ggaaggcata ctcttttggc ggcgcgacag
     9301 catagttacc agcagtggcc atataacgat accagtattg atggcgtcca caatcacagt
     9361 cgaaagatac ccggcccttg cagatatcgg cagcgattcg ggcttttttc gcaccgtctt
     9421 cagcaatatc ctcaacggct ttatcccatt cctcaaatcg aattctgaca cggtgatgct
     9481 ggtggaccga ctcatccgag gcattaacag atatcaatgc aaggttgtgt tttagcccga
     9541 ggaatgtcgc ggctttgatc cctgtgccat cagaaacctt gttgttagcg cgttttatat
     9601 caatgctggt ggactgcgcc accagctgag cataggtaat gccgggtacc gtgctcttga
     9661 atttggtttt atgagcctgc cttgaggtgt tgaaactgcg tatatcttcg ggcgtaaagt
     9721 aggtgccatc tttctttttc ccaaggctga ggaatgcctc aagttcgcgg ttacgcatcc
     9781 ccataatcct tggggtgagt gtacgtcgcg cgtttcgccg attctgacgc tgctgtttac
     9841 ggataagatc gaagaccttg ttaaagtctt ttgcacttaa tccatcagtc tgatagcgac
     9901 caaggttgtc gcgagcatat tcagttggca ttcatttccc ttacgcaatg gataatgtcc
     9961 ctattacctg gccgtcgtat tggaaatggc gaatcatttc gcggatccag gtggcaggtg
    10021 ggagttttaa ttttttgcca acagtcatac cctgagactc atcctcaagc ccggcggcga
    10081 gcgtcacaac ccagcgtagc tctgctatgc cccacatacg gtaagccagc aaatccgggc
    10141 gatattgctc atcgggaaga acgtaataaa tcgtcagatt cttgtcgttc gattcacaca
    10201 taagcatcac ctctttgcgt agctctgccc tgagtattgg atcggctatg ttgcggtcgt
    10261 cataccgcga cagaggatat tgccgggtgc tttgggttgt agtgattgat gtagccatag
    10321 tcagcctgcc agaaatagat gatggtgatt ctaccgctag tcatttgttg attatttaac
    10381 tcaataaaat aaaattatta gtgcaatttt gattgtgaaa tgtatcattc tgcccttaag
    10441 taggttcttc acgaggaaac aaaattggca gaacgtgttg atgatgcaga gctgagcatg
    10501 aatcagttag aagctctcaa agacatggcc atcgataaca tcagaaagca ggcacaggtc
    10561 gtgagccagg tatttacagg gaagtgtcgt tactgcaatg aatcgattga atcaggcatt
    10621 tattgtgacg ctgaatgtgc gcaatggcac agggaagagc aggccgcaaa acagcgtaaa
    10681 tatggcatgc gaccggcagg atttgactga ttatgttgcg ctttactgag gaagagtttc
    10741 aggcttttag tgagcgtcga aataaggggc ggtccaggcc aaaaaccaaa aaggatccat
    10801 tcttatcgct tgcgccggta aaagaagttt ctccacatgc gaaggcactt gcagcacttg
    10861 caaagaaccc agacctgcgc gacggaaatt gcgagcactt cgagcaggtt ttcatttttg
    10921 attacttcga acgcaagcac cctgacatct atgagctgtt gcatgcaacg cctaacggag
    10981 ggaaacgttc aaaagcaacc gccgggaaaa tgaaggctga agggcagaaa aaaggttatc
    11041 cggacatgag tctcgataaa gcatgcggta tttatcacgg catgcgaatt gagcttaaag
    11101 aaccaaatgg taaagccccg acgaaagagc agatcgcctg gatgcgcagg cttagagagg
    11161 aaggttacta cgtcgttctt gcgtatggtg cagaacaagc gataaccgcc atcctggaat
    11221 acataagcct taaaaagggt gaggctattg agcatgtatt gaacggcgac aagtggttgt
    11281 atgctgctta aaataataaa ttaattagtg catgtacgct ctttgtggta gtgcacttta
    11341 acatcgggag aataatcgtg tcatccaagg ttaattatga gtcgctggca tcggtcatgc
    11401 cgcgtaatga acaggaaaca gatgctgtag tggaccctgt aatcgctgaa atgaatgctc
    11461 gcctggaggc tgaatttgca gctgagaatg aacataccac ccagggcgac taggactgtt
    11521 ttttgtgtcg gtagcggtcc gtcactcact cgtgaggact gtgctgctat agaaaaaact
    11581 ggctgttcaa tcatcgcggt taacaattcc tggcagatgt tcgatgacat ttatgcctta
    11641 tacgccggtg atttgtcatg gtggaagcaa tacggatcca ccataccggg agggaaattc
    11701 cgcaaagtga cagccaacct ggcggcggcg aaatcatttt cgttggagta caggcgatat
    11761 tgtggaccgg cggaaggggt aaatagcggc gcgcaggcta tcagtctggc tgctgaatca
    11821 ggggctgaag tagttgtatt agtcggctat gactgttctc tgcaaaacgg ccttcattgg
    11881 catggcgcgc accctcaagc cctacggaat ccaacgcagg tgtctatttc aaaatggcaa
    11941 cagcagttcc tggatacccg caaaaaacac gcagatttac atattttgaa tgcaagtagg
    12001 agcagtgcaa ttcaatgttt cccaagaata aatttagagg cagtgatcgc gttattatcg
    12061 tcggcagtgg cccaagcgcc gcaaactttg ttgcgccgcg cggagtgccg attatagcgg
    12121 tcaatggggc catcgactgg ctgaaccgcg cttcttattt tttcacactt gatccatcgc
    12181 cagacaatat gcggcgcgtt ggtcgtggcc gccgtcgccg tggtgtttgt tattgcatgg
    12241 cactacccga tgttaaagaa cgtgaagtca gagacggcgt tctgtgcttc cgtcgtgtgg
    12301 ctgaacgtgg catggagcca aaaaatatga attctcccga gtggtgggcg tggcgctggt
    12361 ccgcacattt cggactttgc gaagatgaga atgaaattgc cagcggcaat agtgcatatg
    12421 gcgctctgaa cctggctttc catatcggat tcaaacatgt cgccctggtg ggcgttgacg
    12481 ctacacaaga accacgcgtt cactccggcg gcacgccaaa aaatctaagt cacctgcctt
    12541 tgttattcca gtctgcgcgt gaacagattg acgttgtttc atgcgggaaa atgggaggta
    12601 ttccgcagat gactcttaaa gaatggctga agaatacatg atggcaccca caatttatca
    12661 ccgtatcgac ggtaccaaat acaggaatgt ctgggttgtt ggtgatctgc atggttgcta
    12721 caccagactg atgtccgaac tccatcgtgt ggattttgac ccggcgcagg atttactgat
    12781 atcggtcggc gaccttatcg atcgcggtac tgaaaatgtc gaatgtctgg aactattgca
    12841 gatgccctgg ttcagggcag tgatggggaa ccatgagcgg ctgatgattg atgcgttaag
    12901 tccagatggc aacgtgaata actggctaat gaatggcgga caatggttct tcatgctgga
    12961 cactgatcag gaaatattag cctgggcgct ggtggagctg gtaaagcgtc tgccctatat
    13021 cattgagttg aacaccgggc aagaaactat cgttatagcc catgccgact atccggataa
    13081 tgaataccaa ttcggtaagg aggtgccgct tttcaacgtt gtctgggcgc gcgagcgtat
    13141 cagtgattcg atggatgata ttggtggcga aatttcgggc gcagatcgtt ttatctttgg
    13201 tcacactccg gtgaaaagcc cgaagacatt ctggaatcag cagtatatcg acactggtgc
    13261 cgtattttgc ggaaacctga cattgatgaa agtgaaaggt gatggtgcag catgaagatt
    13321 gctttagttt ttcgctctgg tggtgactat aacgcttccg atgtgcagtg gctggttaat
    13381 caactgccaa aaggctatga aattatttgc ctgacagacc tgaagcgttt acatgtacct
    13441 ggcgtcaaag ttgtcccatt gatcaaccag tggcaaaagt gccgtggctg gtgggcgaaa
    13501 atcgagttgt tccgaccgga tataacagat gatctgttct atctggattt ggacacagtt
    13561 attgccggtg atatacgccc aatccttgag aatccaccaa ccagcttcac catgcttagg
    13621 gatttttacc atccacaata tcgtggtagc ggtgccctgt ggataccaaa tagtgtaaaa
    13681 gcgcatatct ggagttcatt ctggcaagat ccggaaggtt ggatttctcg ttgtgtcact
    13741 actgagtgct ggggtgatca ggggttctta cggaaggtta tgggcgatga tacaccagca
    13801 tttcaggatc tgtatccagg atggtttgta agttacaagg ccgatgttgt ggaacctggt
    13861 tcaaaatatg cgagcgcgcg ttactccagg gggaatgggg cattaccaaa ggactgccga
    13921 ataatctttt tccacggcaa accgcgacct cgcgaagtgt cagaggattg gcttcccctt
    13981 attagctcgt tttttgagcg agaatcagaa taatattgct ctaataattc catattttta
    14041 aaacgtgatg tacactcatc acgtttttta ttagagcaat ccaaaaggtg cactatgtgg
    14101 ccattccgac ggaaatatca ctactggctg atcgcctttg ttacgccgac cggcggtatc
    14161 aggcatgtca tcaccaggta tcgcaacaag agactcacct tagccagaat tttacaggct
    14221 gccataggtg agggactgga tacaaattgc gtagtccttc caccttcata cttaggaaaa
    14281 atgaccgaag cacaagctaa tacggaactt tgaaatgagc acttcagcac aaaaccaatc
    14341 aatcgaaaat gtatctatcc ctgatgtcct gaatgccggt atcccggcca ttatccagaa
    14401 catccgggcc gcgcaacgcc gcgttagttg tgatgacctc acagcacgtt tttttgataa
    14461 tgcggttcag tcagcggaga tgcttcacgc acagcttatt gatgtttata acgcagaagc
    14521 tgatagccat aactccctgg tagatgcagc tgaaaatatg cagttggatc tcggtctgaa
    14581 gggtaaagaa attgaagagc ttcagctgca aattgaacat ttgaaacgcc agcaacagga
    14641 cgcgatcgac gatgcgacgc atgacgccaa ccagcgtgct gataatgccg aacgtataag
    14701 cattgagctg gaaacaaaac tcaatgaaat gaccgcgatg gttgaactgc ggaactcaca
    14761 gatttcaacg ctaaaatctc aatataaaga gatcatgaaa cttgatcctt ttaaccttga
    14821 gaaacgctat aacaaagcta aaagcgagcg acaggaactg cgtaagcagg tcgccgacct
    14881 taaccaacag ctcaaaaaaa ctattaaaga tgcaagcgaa gcgcgcgtgg catttgctaa
    14941 taaaaaagca gaggttaccg cgctggttaa tgagaatgcc aaatttgcga cgctcaagaa
    15001 ggaaatgtat ggcattactg agcgccgttt ccctgcaagc aaacttcatc cgacgttagg
    15061 gcaaatctcc ttcttcccgc gcctcctggc ttatgggatc tcatcgccta aagagttcaa
    15121 taacgagcgt ccttatatcg tttctaagct ggactttgct tatcagttct gctgcgacat
    15181 gggctatgcc attgatatcc ggatcaacga atggttgatg ccaaacttcc agccgttggc
    15241 cattttccgc gagttccagc cggaaggttg ggtagagttc ttccatgaat tgatctgtaa
    15301 agagatggaa agccgccgcc cggaactggt ccgtcgagtt gagtgggcgc aagaggttat
    15361 gttggcagag gcagagctgc cgttcgaacc ggaattcatt gatgaactgg ccaataaagg
    15421 gctgcatagc ctatttgatg tagttacccg ccgtcatgag cagttggttg ttgaattggg
    15481 tttagaggaa actgcggcaa gaagacttct cgatgtttgc tatgcacgta gcgatgcatg
    15541 ggaaaaagag aacggcggca ctatttacgt tcgctgatag ttacagtgtc acatttaatg
    15601 ctggtggagt gcgcccacca gcattttttt cgtccaatga ggagggcatt tgagtatttt
    15661 caataaacac gcacaccagg aacgtccgta catcgtcata gtagatattg atgggacgat
    15721 atcagaggca acggaagaca ggctgcattt acttccacca cctggcaaag gtgcattaac
    15781 agagcactgg aacgagttta accttgcctg tgacactgat gctcccatca ctccagttat
    15841 tgatatggtg cgccagttgt ccggcattta caccctctgg tttgtaaccg ggcgctgtga
    15901 gatagccagg gataaaacac gagcctggtt gcgtaagcac gtaacaaatg gggctgagcc
    15961 tttgctatct atgcgtcctg ccaccgatga cagaaatgac ggtccagcaa agattgatct
    16021 cctgaagaaa attggtctaa gtaaaattgc gttcgcgctg gaagataaga ttgaagtggc
    16081 gcgtgttttc aggagtcacg gcgtacttac gttaatggtc agggagtatg aaaacgcgct
    16141 tctccaccaa caataattgc tctaataaat cttgattttt aaaacagaga aagtgaaaat
    16201 aaaaacatgc cgcaaggcgc ggcatgtatc caatcaatca caggagctga aaatatgaac
    16261 acggcattca aaatcattat ggccgcgatc tatttctggc tgttctctat cacttttggc
    16321 ggcatcgtcg cacacgggta agggggatgc atgaaaggcg aagtgaaaaa gcgcggcatg
    16381 atttttaacg atgagatggt ccgggccatc ctcgaaggaa ggaaaacgca gacgcggcgg
    16441 ataatgaaaa atcagcctgc ggaagttggt ccagaagcac cagtgatggt tagaaaaatt
    16501 ggtgcaggtt ttcagtggta cggggctgat ggtgtaagca gtgttttcaa ttgccccttt
    16561 ggtatcgtcg gcgatcgaat ttgggttcgt gaaacatggg cgatattagg caatgaagat
    16621 ggttgcagtg tggactggaa cgacaacctt tgtcgtggcg atgagaagaa cgcagcaagg
    16681 atttatcggg ccagttgtga gcaaaagcct ggtgattacg gtttatggtc gatacccgat
    16741 gatgccgact ggaaacctca cactgtgaat gaaaagtttg atggtgggtg gcgtccatca
    16801 attcacatgc cgcgatgggc atcacgcatc ctgttggaaa ttaccaacgt gcgcgttgag
    16861 cggttgaacg atatcagcga atgcgatgca agggatgagg gtgttccgcc tgctggaagt
    16921 ttgcttcctg atcacccggg aacattcctt actcccaagg gggatttcgc aatggccaag
    16981 gttgcgttcc agcgcctgtg ggaatccatc tacggcgaag aaagctggag cgctaatccc
    17041 tgggtttggg taatcgagtt tgagcgtatt cagtagggcg agcgtatgca atcagttatt
    17101 tgtgaggaag tcggcctgaa taaagcatcc ccagcagagt taagagcaag tctcgaaatg
    17161 gcgcatagcc ttgctcaaat tggtgtcagg tttgtaccaa ttccagttga aacagatgaa
    17221 gaatttcaca ggttagcggc atcggcagca caaaagctgg aaatcatggc agcgaaagta
    17281 gagaaagctg aaggagcgac aaaatgagca agccaaccga cgaagaaata gttcgggtgt
    17341 tggaagaaca cgggcgctgt atgacttacg tcgtgaccaa ctggcttagg gataaatatc
    17401 gcacactcaa aacggcatac gttttgcgtc gtctaaagaa actggagttc gacgggaaag
    17461 taaagcgcgt taacagttct tatataagac aaatttgctg ggaggcatcc agtgagtaac
    17521 cgtttttaca tgatgtgctt gcgtgaaact gtgggtaata acgcctcatt ccattgccat
    17581 aacggcaatg gttacagttc tgatatcgat cgcgctcatg tttacacgct ggaagaagcc
    17641 caaaaagcct ggaattgtgg gcgagatatc gatcagcctg tttgtgccga tagtgtggat
    17701 gcaatggctg tgtggcacgt tgattgccag tacatcccta cagaaagcct gattgagtca
    17761 gattgcactg cgtatgtggc ctacaaaaaa ggtagctgga acggcaacga tgtttactgg
    17821 cttcaacacg gtggattgcc aacagatgac ttcagtaaag cgaccatctt tagcgtcgcc
    17881 aacaaaaacg aaccaggaat agtttggttg ccattttcca ttgctgatgc agcaaagcgt
    17941 cggacgttca atatcaataa ctttaaccgc agaacaatgg ttcagggcgc aggtttggtc
    18001 atgcctgact ggttgaaaga gcagaacaga agaaagaagt cgcgaagcgg gaaggtgcgt
    18061 tggaattgtc cgcattgcgg aaaaataacc tggcagtaca gcccatatga ttttgaaggc
    18121 tgtagtgatt acaactgtga aggatggcga gaatgacaat tgactatcag gcactgcgag
    18181 atgcggcagt tgcagtagaa acagaaccta tgcatcaaaa ttttgtggcg tttcgtatgg
    18241 cgttcacgcc atcggtggca ctggcactac tggatgaaat taagcggctg gaagacacaa
    18301 atattgatgc tatgtgccgt attgcagagc ttgagactaa tctcgcggcg ctggtggcag
    18361 agaacgccgg actgaaacac gcaatggccg taactcttga gcatgtgtcg gtcacggatg
    18421 cagggcaggc tggtgttgct gcaatgatta tcaacgatgc cctacaccac agcgaaactc
    18481 cagctaccga tgcttttctg gctgaaattc gtgcggaagc acgcaacgag gggattaact
    18541 ataccgccag ccgtcttgct gctgcgttca atcacggatt tatcaataag tctttgcgtg
    18601 aagttttcga cgttacacgc atgattttgt cagcgaaaga agagttggct aatgaagcgc
    18661 atccgattga tggcctgtct ggtgaatatg cggagaaatc ccttgaagaa tgggcggaac
    18721 agattcgcaa aggaagcagc cagtgaatat cgacacgaca ataacgatcg atacgctcct
    18781 aaataccggt ctggcacttc tcggttggct tttcatcatg tcccgtacat ggcgatggct
    18841 gggttccatt ttcctaaaac agtggaaaaa acggcgcaaa caggaactac gccagaaggc
    18901 attagaagcg ttctatgacg catttgagct tagccgcatt gaaccaggta caacagctag
    18961 gatagcgaca aaaggcgacc tgatgatagt gatgttccga caggagagag cagagaaagg
    19021 ggaatcagca tgaaattttc caaattttct gagttggtga atcgtatttt gttcaacaac
    19081 cacagccatc gtcgcgatat ggatgtaaca atcgttgttc attcgcctgg cagcattggt
    19141 tcaacaccct cagttgaggt tcagtcaatt cacgctggtt ttgattggga ttccgggaca
    19201 gtgctgattt tcccagcaca gccactgacc acgctaacac cagaacagat tactgatatt
    19261 actgatagtg tgcgcaaagg tcagtcctgg cacgcatatc aggaatacaa gaagcataaa
    19321 gagcagttgg aaaaattatc gattgaactt gatgccgcaa aacagcgcat tgcagagctg
    19381 gagggtaatt gcgcggcgct ggcggcggag aatgcggcaa tgcatgaaac tattgaagcc
    19441 gttcggggtg ttgcggataa ctccagtgga attgccggat ggcatttgaa tggcgatatc
    19501 gctacatggg aagagattct tcctgaaatt aacgatatcg aaaccccagc caccgatgct
    19561 tttctggctg aagtacgtgc gcagggcgtg gagatgctgg caaaaaatca tcagagtatc
    19621 gtcaatgcac ttaagggaga ttctttattt tctgatggtg aataccgaca tgcagctatt
    19681 gtttccgcag ctgtatattt cgctgcagag cttcgcaaag gaggcaacca gtgagcgaaa
    19741 ttaattacca ggcactgcgt gaggcggcag aacgtgcaac tccagcaatg gaacgcctgt
    19801 taatgttgcc agttgatgat gatttgttaa gtgaacagga acttaaagat tacggtgtgg
    19861 atattgatgc gctcaacgcc ttcaaatttc tgaccggacc agaaaccgtg ctggcactac
    19921 tggatgaacg ggaaagaaac cagcaataca tcaaatcacg cgaccaggag aacgaggaga
    19981 ttgcgcttac ggaagggaag ctgctaatcg aaaacggccg gcttgttgcc gatacgctac
    20041 gccacttagc tgataacgaa atcgactctg attattttgc tatcacctca acgaatgaga
    20101 acggtactga aattgatcat gagatggcta ttaccgatta cgcactgcaa gctgccggaa
    20161 ctgtagacga attggttgcg gcgttggaat ccgcagagaa tcgcattgca gaactggaag
    20221 cacgggaaat atcgctccca gaacgtagca gcatgcttca tcgaacagat tttcacgatg
    20281 attaccaaac ggtaatggca tacaaagttt ctgaagtcat cgctgcaatc cgcgccgctg
    20341 gcattcgcat caaaggagag gagcatggaa ataaaaccag aagatgagtt aagcaatatc
    20401 gttttatttc cggtaaaaga ggatgaccca cgtaatcagg ttaattttct ctatgagcca
    20461 tcggaaagac catattgcca tcacgcctct gtccgggttg acgaaaaaga gcgtcaggtc
    20521 cgctgtaaaa tctgcggtg
//
LOCUS       DAZFDP010000079        20318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFDP010000079 DAZFDP010000000
VERSION     DAZFDP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..909)
                     /gene="cutC"
                     /locus_tag="ACHJ1G_002953"
     CDS             complement(163..909)
                     /gene="cutC"
                     /locus_tag="ACHJ1G_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4764770.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(923..1489)
                     /gene="yecM"
                     /locus_tag="ACHJ1G_002954"
     CDS             complement(923..1489)
                     /gene="yecM"
                     /locus_tag="ACHJ1G_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4764771.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1705..3438
                     /gene="argS"
                     /locus_tag="ACHJ1G_002955"
     CDS             1705..3438
                     /gene="argS"
                     /locus_tag="ACHJ1G_002955"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4764772.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3480..3524)
                     /gene="yecV"
                     /locus_tag="ACHJ1G_002956"
     CDS             complement(3480..3524)
                     /gene="yecV"
                     /locus_tag="ACHJ1G_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO4764773.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3615..4103
                     /gene="yecT"
                     /locus_tag="ACHJ1G_002957"
     CDS             3615..4103
                     /gene="yecT"
                     /locus_tag="ACHJ1G_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4764774.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4223..4615)
                     /gene="flhe"
                     /locus_tag="ACHJ1G_002958"
     CDS             complement(4223..4615)
                     /gene="flhe"
                     /locus_tag="ACHJ1G_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4764775.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4615..6693)
                     /gene="flhA"
                     /locus_tag="ACHJ1G_002959"
     CDS             complement(4615..6693)
                     /gene="flhA"
                     /locus_tag="ACHJ1G_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4764776.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6686..7834)
                     /gene="flhB"
                     /locus_tag="ACHJ1G_002960"
     CDS             complement(6686..7834)
                     /gene="flhB"
                     /locus_tag="ACHJ1G_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4764777.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8036..8680)
                     /gene="cheZ"
                     /locus_tag="ACHJ1G_002961"
     CDS             complement(8036..8680)
                     /gene="cheZ"
                     /locus_tag="ACHJ1G_002961"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4764778.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8691..9080)
                     /gene="cheY"
                     /locus_tag="ACHJ1G_002962"
     CDS             complement(8691..9080)
                     /gene="cheY"
                     /locus_tag="ACHJ1G_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4764779.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9095..10144)
                     /gene="cheB"
                     /locus_tag="ACHJ1G_002963"
     CDS             complement(9095..10144)
                     /gene="cheB"
                     /locus_tag="ACHJ1G_002963"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4764780.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10147..11007)
                     /gene="cheR"
                     /locus_tag="ACHJ1G_002964"
     CDS             complement(10147..11007)
                     /gene="cheR"
                     /locus_tag="ACHJ1G_002964"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4764781.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11026..12630)
                     /gene="tap"
                     /locus_tag="ACHJ1G_002965"
     CDS             complement(11026..12630)
                     /gene="tap"
                     /locus_tag="ACHJ1G_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4764782.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12676..14337)
                     /gene="tar"
                     /locus_tag="ACHJ1G_002966"
     CDS             complement(12676..14337)
                     /gene="tar"
                     /locus_tag="ACHJ1G_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4764783.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14482..14985)
                     /gene="cheW"
                     /locus_tag="ACHJ1G_002967"
     CDS             complement(14482..14985)
                     /gene="cheW"
                     /locus_tag="ACHJ1G_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4764784.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15006..16970)
                     /gene="cheA"
                     /locus_tag="ACHJ1G_002968"
     CDS             complement(15006..16970)
                     /gene="cheA"
                     /locus_tag="ACHJ1G_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4764785.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16975..17901)
                     /gene="motB"
                     /locus_tag="ACHJ1G_002969"
     CDS             complement(16975..17901)
                     /gene="motB"
                     /locus_tag="ACHJ1G_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4764786.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17898..18785)
                     /gene="motA"
                     /locus_tag="ACHJ1G_002970"
     CDS             complement(17898..18785)
                     /gene="motA"
                     /locus_tag="ACHJ1G_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4764787.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18912..19490)
                     /gene="flhC"
                     /locus_tag="ACHJ1G_002971"
     CDS             complement(18912..19490)
                     /gene="flhC"
                     /locus_tag="ACHJ1G_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4764788.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19493..19843)
                     /gene="flhD"
                     /locus_tag="ACHJ1G_002972"
     CDS             complement(19493..19843)
                     /gene="flhD"
                     /locus_tag="ACHJ1G_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4764789.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4875 a         5582 c         4947 g         4914 t
ORIGIN      
        1 aatattatta gcacaacgct atgttcactg tcacccgctc aacagatcac ggttatcgtt
       61 cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta
      121 cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg
      181 ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc
      241 gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc
      301 ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag
      361 gttttctgca cggactcctg ctccggccat aatgattgga gcatcacgat gggcaataag
      421 ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg
      481 ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa
      541 ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg
      601 catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc
      661 gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc
      721 accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt
      781 cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct
      841 gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag
      901 taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg
      961 accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta
     1021 atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc
     1081 cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa
     1141 cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct
     1201 ccacaataga aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa
     1261 tcggtctgcc attgatcata ttttctgaca aaagctcgcc acactgttca aacccgcgac
     1321 gccagcgttc agcagtggcg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg
     1381 gagtgatatt caaccccaga cggagggaaa gttcatctaa tgcgtgggta aatcgcggta
     1441 aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc
     1501 gtctttcagt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc gctgatttca
     1561 cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa
     1621 cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc
     1681 gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt
     1741 caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg tcagtcagca
     1801 aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt
     1861 atggcaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc
     1921 agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg
     1981 gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgtttctac gccagaaaaa
     2041 cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca tgtcggtcac
     2101 ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct cggtcacaaa
     2161 gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg
     2221 ctggaaaagc agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc
     2281 taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt tcgccgagcg tgcacgtaac
     2341 tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc
     2401 gacatcacca tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt
     2461 gatgacgtga tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc
     2521 aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt
     2581 aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc
     2641 tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aatcactgca tgccgatcgc
     2701 gtgctgtatt acatcgactc ccgtcagcat caacacctaa tgcaggcatg ggcgatcgtc
     2761 cgtaaagcag gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg
     2821 ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg gtacagtgaa actggccgat
     2881 ctgctggatg aagccctgga acgtgcacgc cgtctggtgg cagaaaagaa cccggatatg
     2941 ccagctgacg agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg
     3001 gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt
     3061 gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt
     3121 aaggcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt
     3181 gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt
     3241 gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc tggctggtct gttctctggc
     3301 ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg
     3361 aaactggcac aactgacagc gaagacgcta aagctgggtc tggatacgct gggtattgag
     3421 acagtagagc gtatgtaatc gatttttcgt gagagtaaag cctgatcagg gttacctgat
     3481 caggcttttt tattgccatc taaatgtatt ctgaaaatgg acatgccatt gttttctgac
     3541 tgttggataa gaggccagaa gcgtaatatc cggccccagg gaaacgataa cggttgaatt
     3601 taaggaatac cgcagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg
     3661 cttacgcaga agatacggtt atagtaaacg accatgacat ttcagccatc aaagattgtt
     3721 ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag
     3781 aatacaatct cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag
     3841 aacaagtgcc acgcacaggt gtaaaaaaac ctgataccga acaacttaac ttgcttaaaa
     3901 aatcacagcg agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg
     3961 aggacgttca ggatttaagt gacccttatt ctgaatcaga atggctctca tgtatgatca
     4021 tacagaccaa tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc
     4081 caagcccttt gacaagagga taattcacat ctttttggca tgttttgttg caagctattc
     4141 ctgataaata attgcaacaa gacatcgagc ctttttcact gagttattaa acatactcgc
     4201 gagcgcgtaa tttttttgtc cttcagcgat aattcacaat cacttcatta cgttgtacct
     4261 ttagcggtgg aattaaccgc ccaccgcctg gcacttccca gataaatcgc aacggttctg
     4321 ctgccgctat accggaaaag gccacggtgg ttccgctctg cccctctaat tcgacacaac
     4381 gagattgcga acacaagcga acccgcagtc ctgaaggtgt cgggccgata agctgataac
     4441 gccacgctac cagcgtcatc aatcctgaag caggttgtcg cgtagaaaga ggcgcagacg
     4501 acatcgactc accgcgatga tttagcgtaa ttcctacact acttgcctgc cacatcccct
     4561 ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggtt ctcattattt
     4621 gccgccaatt gtcgccgtca tgcggatatg tcggttatca gacagttcca gattcgacag
     4681 gaccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcggcgcgtg
     4741 gttcaccaac aatactggcg gcgcacccag catctcctga cgggatagcg cttcctgagt
     4801 ttgcgccagt aaacgatccg ccagccctgg ctccagtcct cccccgccct gcagcgcctg
     4861 tagtaacaaa cgttccagcg gtgtatcgag gccaataaca tggacttcat ctttgccagg
     4921 aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg
     4981 tggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatggtgc gcatatcgcg
     5041 aatcggtact ttttcatcga ggagattttg cagcacttta tgcagtgtgg tgagcgtgac
     5101 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag
     5161 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
     5221 atgcgttgct accaccgtgc tggcctcaac cactgtgtac ccctgaatct gcgcctgttc
     5281 ttttagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
     5341 accaggtaac gtcccggcag cggttccagg gttaatcgcc agccagcgcc ccggataagc
     5401 atcaccactg ccaatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagctc
     5461 catattgtcg cgaatgtgca ccactggcgg cagaaatccc atctcctggg caaatttctt
     5521 gcggatactg cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat
     5581 cagtcgataa cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc
     5641 ttcgacaacg gtattattct ctgccatttt taccggtttg ggttcgacag gcgctttttg
     5701 ttcgcgtccg cgtatccacc aggccagccc cagcaatccg gcagtgaaca gcaaaaatac
     5761 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat
     5821 aacgcttggg ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
     5881 gacacgcgta acgatgaccc ccgcggcggt agaaatcacc agcgccggaa tttgtgccac
     5941 cagaccgtcg ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc
     6001 atgttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc
     6061 ggcgatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc
     6121 ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt
     6181 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc
     6241 cacttctgcg atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat
     6301 gacaaacact acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaatgcttc
     6361 gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga
     6421 agccacgtta agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc
     6481 aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat
     6541 attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa
     6601 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc
     6661 gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
     6721 gggcttccgg caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc
     6781 agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg
     6841 ggatttgttg accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa
     6901 gcgtcgggac gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac
     6961 ctttagcgac cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat
     7021 agtgggtcgg gttattgaca atgacatccg ctttcggcac atcggccatc atccgacgcc
     7081 gtgcagcagc tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt
     7141 gtttgaactc atcacgaata tcctgccgtg acatacgcag ctttttcagg tggctgaata
     7201 tttggaaaaa gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc
     7261 atagccctac caaatccatc gcattaccca tggcggtaat cggagactcg gccatcaagc
     7321 gcatcatctg cggccagtga tgccagagaa aaaaccccgt cacgctgcca accaggatgg
     7381 ttttcaaaat tgctttaagc aactccgcgc cagtctgagc cgagaacatc cgtttaatgc
     7441 ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc
     7501 ctcccagcat gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca
     7561 gcgccagcat ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtcat
     7621 tgataatact gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca
     7681 gcgacacacc gccaaaccag ataacactaa cgcccaccag caaaatcagc agtgaggtca
     7741 gttcacggga acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg
     7801 gggcttctgt tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa
     7861 gcgtgaatga tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg
     7921 gatttagggc ttttgctgcc acacatcaag catagtgtgc gtttgtcgga tgcggcgtca
     7981 tcgccttatc agaccgcctg atatgacgtg gtcacgccac atcaggcaat acaaatcaaa
     8041 atccaagact atccaacaaa tcgtccacct gatcctgact ggctaccaca ccggctttgc
     8101 tggtatcgac ctgaggtcca ttaagcaaac tctggttttc acgttttgga cgcgactcct
     8161 gttccgggat gttttccaac agcaccatca gcaactggcg ttcgatctcc tgaatgacat
     8221 ccatcatccg cttaatgacc tgcccggtga gatcctgaaa atcctgcgcc atcatgattt
     8281 ccagcagttg cgcgttagta aagctggtat gcgcgggtac atctgccaga aattgtcgtg
     8341 tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc
     8401 aacgttgggt taacgctttt gctgatttct ccatttgatc ctgatgcggt tgtgacgcct
     8461 caacactgtt cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca tagtacaaac
     8521 gatcgcgcgc atcggggatg gcttccgccg cttcggcaat ggcctgatcc agccccagtt
     8581 cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg atatcgccag
     8641 ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc tcacatgccc
     8701 agtttctcaa agattttgtt gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc
     8761 acatagccac tggcccccgc ttgcgccgca gcaatgatgt tctctttctt cgcttctgca
     8821 gtcaccatta acactggcaa tgccgacatc gcgccatccg cacgaattgt tttcagcaat
     8881 tccaggccat ccatattggg catgttccag tcggagataa caaatccata accgcctgcc
     8941 tgcaacttat tgagagcgtc gacgccatct tccgcttcct caacattatt gaatcccagc
     9001 tctttcagca ggttacgcac tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat
     9061 ttaagttctt tatccgccat ttcacactcc tgatttaaat acgtatcgcc tgtccggcac
     9121 taatttttgc caacatttgc tggcttacct ggctaagatc gaccacttcg cagacaccac
     9181 ccatattgat ggcctcgcgc ggcatgccga acaccacgca acttgcttcg ttttgcgcaa
     9241 gggtccatgc ccccgcctga cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac
     9301 cggtcaggat caccccaacc gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca
     9361 catctaccga aggccgatga cggttaaccg ccgggccatc gtgaattttg atttggtaat
     9421 ttgcgccact acgcgacagc tccatatgcc gatcgcccgg cgcaatatag gcatgccccg
     9481 gcaagacacg ttctccgtct tcggcttctt taaccccgat ctggcaaagc ttattaagtc
     9541 tgtcggcaaa agagcgggtg aaaccgggcg gcatatgttg ggtaattaac aatgccgggc
     9601 tggaaagcgg caacggttgc agtacgtgac gaattgcctc agttccaccc gttgaagcac
     9661 caatcgcaat cagtttttca gaactcaaca acggacccgc cttcagcgtt gtcggtgctg
     9721 acaatggctt atgtgctgca aggctcgcct ttgctgccgt acgcaccttt tcagcaatca
     9781 tttcgctata cgccagcatt ccttcgcgaa tacccagttg cggtttggtg acaaaatcta
     9841 ttgcccccag ctccagcgcg cgcagcgtga cttctgatcc tttgccggtc aaggaagaaa
     9901 ccatcacaac gggcattgga cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca
     9961 tccgcggcat ttcaacatcc agcgtcagca catcgggatt gaatttctta atcaagtcac
    10021 gcgcgaccag cggatcaggc gcggtcgcca ccatttccat gtcgctatgg ctgttgatga
    10081 tttctgtcat gatctggcgc atcagtgccg aatcatcgac agataacacc ctgattttgc
    10141 tcatcgttaa tccttactta gcgcatacac cgtctgacca cgcagcgtga agcggcgctc
    10201 aaggtggcta aagttttcag agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg
    10261 aacaaagcgg cgcaaaatct cctgctgggt attttgatcg aagtagatca tgacgttacg
    10321 acagaagatc gcatcaaacg gccccggcac ggtgtactgt ttcgccagta gattcagcgg
    10381 ggcaaaatca acatagttcg ccagctcctg acgcacgcgt accagccctt catgcggtcc
    10441 cgtccctcgc atgaaatacc gttgcagttg ctgcggcgtc aggtttttca actcttcatg
    10501 gcgataaata ccgcttctgg ctttttccag cacttcggtg tcgatatcac tggcaaacac
    10561 tttccagcgt ccgggcgcgg tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg
    10621 ctcttcgccg gtcgaagccg ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc
    10681 gtgatccgcg agcagaggga aatggtgtgc ctcacggaaa aatgccgtca gattcgtggt
    10741 cagcgaattg ataaacgcct gccactcacc gctgtgctga ttagattcca gcaagttcag
    10801 ataatgaccg aaatccgtca gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac
    10861 catgtcgcgt ttatggtcag ccagaacgat cccggctcgt tgatagatca attgacttat
    10921 ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca ataaagacgt
    10981 ttgcccacag ggcagagatg aagtcatagc gccttctcaa tcacttcagg ataccactgg
    11041 cgcaatttgt aactgcgccg actcatgtcg tgctacttca tgttcttcaa gggtaaatac
    11101 cgccacacgc gacgaaagat ggtcggcctg attcgccagt tgttccgttg ccaccgccgc
    11161 ttcttctacc agcgaggcgt tctgctgagt cacctgatcc atctggctga cagcctgtgc
    11221 aacctgctca atcccccgct gttgttcttc cgacgcagag gcaatttctc ccataatgtc
    11281 gtccacgcgg gtcaccgaac tgacaatatc gatcatggtc gcggcggcgt tattcaccag
    11341 tttcgaaccc tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc
    11401 tgcctgcgcg ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg
    11461 cccctgctct ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg
    11521 gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat
    11581 ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc
    11641 ggcatttttt gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc
    11701 ggttaattgc tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg
    11761 acttgagaga tcgttattgc ctgcgacaat ctccgcgata ccaatgtgca tctcctggct
    11821 tcccttacgc acatcactca ccgtcccacg caaagcctgc tgcatggtct tcaggctggc
    11881 aaaaatggcg gtgatctcat tacgaccata taccgcaatc ggacgcgcca gattacccgc
    11941 agcaatgctg tcaaaatggc taccgataat ggccagtggt tgaacaatca tcttgcgcgt
    12001 ccaccacagc gcactgctga tgtagatcgc cgcaacaata atcatgctga taaacaccag
    12061 cgccgaaatc tgatagttac gctggctttg cgcactggcg gcttccagca catggttgat
    12121 ctccagctgc caggcctcaa agttaacgtc aaacgcattc tgtgattcct gcaccggcgc
    12181 agtgaggaaa tccgaaagtt gattactttc aagccaggtc gcctgatgtt cgagatcgtt
    12241 gttgtgccag cgggcaaaac ttttctccgt ctctttttgc aacgccctga cgtgctcgtt
    12301 gcccgcagtc atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg
    12361 cgccgtcgtc atcaacgttt taatgtcatc cgccggatag ctaagtgccg tcagagtgcc
    12421 cgctttgttc agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc
    12481 acgttgctga ttgctctgct cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca
    12541 aaaagacatg ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag
    12601 cgtggtcgaa attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc
    12661 gttagtaaat actcgtcaaa atgtttccca gtttggagct tgttcagcaa ttcgcagtcg
    12721 tggctgtgcc ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag
    12781 tgggctggct gccagacgga acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc
    12841 cagcgcagcg gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg
    12901 atccatttcc gaaaccgcca atgcgacttg atcgatgcca cggctctgtt catccgatgc
    12961 cgatgcaatc tcgcccataa tgtcagtcac gcgagtgaca gcattgacga tattgttcat
    13021 tgtttccccg gcgctttcga ccagcaccga accggtatca acgcgtgaga cggagtcttc
    13081 aatgagggct ttgatctctt ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc
    13141 acccgccacc acggcaaaac cacggccctg ttcacccgca cgcgcggctt caaccgcggc
    13201 attcagcgcg aggatattag tctggaaggc aataccgtcg ataacgctga taatgtcggc
    13261 aattttcttc gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt
    13321 gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt
    13381 atcggcgttt tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag
    13441 cgcggatgcc tgctgttcag tacgggagga aagatcggtg ttgcccgccg caatttcacg
    13501 ggtaccggca tagatggcat ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga
    13561 gcgttgcata tgtgaaacgc tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt
    13621 cagggtattc gccaggttac caccggcgat ttcgcgaatg tgagcaataa tttttgccag
    13681 cggagtaagc aacatacggc gaatgccgta ccacgccacc agcagaatca ataccaccac
    13741 cagcgcgata accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac
    13801 gatatcgcga tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc
    13861 attttgcatt ccctgggttg gctgagcgaa ataagctcca gtattgccat aatcaagata
    13921 atcaatcagt tcagttaacg ctgtgtgata gtttttatat ttttcatcaa tattacgact
    13981 ggtagcgacc atttcaggta acggtgccat gcttttgaat tttttataat gagtcgcggc
    14041 ctgggccaat gttttcctgg cgctatcgag caattcaact ttggcgttac tttgttgatt
    14101 ggaggaatcc atcatcatcc gcaccgctga acgactcagg ttaatgcgcg tttgcagcat
    14161 taaatcccag gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa
    14221 gctcttctgg ctatggtgaa gggaagaaaa aaacagactg ccggaaataa gctgtaacag
    14281 tgcgaatacc cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag
    14341 gcaccttcct gaaaacaagt taatctcgtt atcggcaaga aggggggaaa ctttattgct
    14401 gatgccaccc gccgcgaaat tgaaataaaa aacccgatgc gcagatcatc gggttcattt
    14461 caattgagga aatcgggaga attacgccac ttctgacgcc gcgctatcta acagcgccat
    14521 ctcttcgctg ttcagcagtt tttcgatgtt caccagaatc aacatccggt cgcccagtgc
    14581 gcccagtcca gtgagatatt ctgttgaaag cgtcacggca aactctggtg ccggacgaat
    14641 ttgctccgcc gtcaatgaaa gcacgtctga aacgccgtca accacgatgc cgaccacccg
    14701 ctgtccgaga ttcaggacga taactaccgt gttgtcgtta tagtccacat ccacctggct
    14761 gaacttaatt cgtaagtcaa caatcggcac aataacgccg cgcagattcg tgacgccttt
    14821 gataaacgct ggcgtgttcg caatccgtgt tacctgatcg tagccacgga tctcctgcac
    14881 tttcaggata tcaataccgt actcttcatc accaagggta aataccagaa attcctggcc
    14941 tgacggctcg ctggccagct ttgttacatt cgtcataccg gtcatattgt taccttttta
    15001 ctcattcagg cggcggtgtt cgccatacgt tgttcgcggt ttatcgcctg caaggcggag
    15061 acatcaacaa tcagtgccac gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg
    15121 actttgcgat agttactttc aaggttttta accacaacct ggtgctgacc aattaattga
    15181 tccaccagca aggcgtagcg gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg
    15241 gcttcggttt tcgcgcccgc gacgttgaac actttccaca gttcgacgat gggcagatat
    15301 tcaccccgca cttccagcac ccgctcgccg ccggccagtg gatgcagatc ggcttcacgg
    15361 ggttgcagtg attccataac agcattcagc ggcagaatga aaacttcatc cgcaacgcgt
    15421 acggacatgc cgtcgaggat ggccagcgtc agcggcagta aaatgcggat cgtagtgcca
    15481 gtaccctgct tcgactggat ttcgacatga ccgcccatct cctggatatt acgtttaacg
    15541 acgtccatgc cgacgccgcg cccggagacg tcggtgacct gctctgccgt ggagaagcct
    15601 ggtgcaaata tcagcatcgc gacttcgtcg tcgctcatgt tttcgctgac agtcaaacct
    15661 tgcgaggccg cttttgccag aattcgctca cggtttagcc ccgccccatc gtcggtcact
    15721 tcaatgcaaa tgttgccgcc ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt
    15781 ttacctgcgg cgagccgttt ttctggcagt tcaataccgt gatcgaggct attgcgtacc
    15841 aggtgggtca gcgggtcgat aatgcgttct atcaggcttt tgtcgagttc agtagaactg
    15901 cccaccagcg tcagttctac ctgcttgccg agttttcccg ccagatcacg caccagccgg
    15961 ggatagcgac taaaaacata ttccatcggc atcatgcgaa tcgacatcac tgattcctgc
    16021 aaatcacggg cgttacgttg taactgcccc atgctggtta tcaaatcacc atgattaacc
    16081 gggtccagtt cgctggaacg ctgggcaagc atggactggg tgataaccag ctcgccgacg
    16141 aggttaatta attgatcaac cttttctacc gctacacgga tgctggtgga ttcattgctg
    16201 cgcgtcgttt tttcccgctc cacgcggccg gttggcgctt gttcggctgc cagtttaagc
    16261 actggtgggg tggatatttt tggcgagact tctactgttt caaaggtaat ctgatcggct
    16321 tcaatcacaa aacagagtac cgctgtgatg tcatcttcgg cgatgtcgcc cggtaatatt
    16381 gccgagagcg aatccgcccc tttcaccacg tcagttaacg ttgtcagatg tcccagttct
    16441 tcttccagca ggtcgacttc cccggccttc aggcgcgaaa ggataattcg tcgcggcgac
    16501 tgactgcgac tctgctcatc ttgcggttca cttttggcaa ccacacttaa tcgggtcact
    16561 gcggatggcg tttcgccttt cgcttctaat gccagttgac gcaaggcctg gcagatataa
    16621 tcgaagctgg cggcatccgg ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg
    16681 atgtccttcg tttccaaaaa cagattgata atgtcggtgt tgagttgcat ctcacctcgt
    16741 ctggcttcat cgagcaggtt ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca
    16801 aaagttcctg cccctccttt gatcgagtgg gcagcccgaa agatggcatt caattgttcg
    16861 gcatctggcg cttccggctg caaaaccagc agatgctgct ccatgtcagc caacagttcg
    16921 tccgcttcat caaaaaatgt ctgataaaaa tcgcttatat ccatgctcac gctgtcacct
    16981 cggttcggct gatggcattg tgggaacact gacctgtggt gcaacctcag gtttttccag
    17041 ggcgcttact ggctcattct ggctttcggc gttttcatgc aaaatggcct gttcggcttg
    17101 tttgttcagt accagcaggc tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct
    17161 taagcgcatc gttgccgcca tgccgacgac acgtaacact ttgccgctat ccaaccctcc
    17221 gaccatcagt tcgcggcggg atgcattggc ccgatcggca gaaagctccc agttgctata
    17281 tcctttctca ccgctggcgt aggggaaatc atcggtatga cctgaaaggc taatacggtt
    17341 gggaataccg ttcagtacag gcgcaatggc acgcagaatg tcgcgcatat agggttcgac
    17401 atcggcactg ccggttctaa acatcgggcg attctggcta tcgatgatct gaatacgtag
    17461 accttcctgg accagatcga ttttgagatg gggacgtaac gcccgcagtt tcggatcgga
    17521 ctctatcaac tggtcgagat caccccgcaa tttccgcaat cgactttgct ccatgcgttt
    17581 tttcagctct tcgatgttcg gctgcttatt cacttccccc tggctttggg tgtaatcatc
    17641 accaccgccg ggaattgggc tttcactatt agaaatgcga tcgccgcccg taaccgcagt
    17701 cgccagtgga gtccggaagt actccgcaat ctgaatcagc tcttttgggc tggagatgga
    17761 gatcagccac atcaccagaa aaaaggccat catcgcagtc ataaagtcgg cataagcaat
    17821 cttccacgat ccatgtgctg ccccgtggct tttggctttg cgtcgtttga cgacaataat
    17881 cggatgcgct tgattcttca tgcttcctcg gttgtcatct gttgttgcgg atttttcacc
    17941 gcacgcacat gctcttccag ttcaataaac gacggacgtt cgctggaata gagcgttttg
    18001 cgaccaaact caacggcgat aggcggtgcg taaccgttca gattagaaag cagagtgact
    18061 ttgacgcact gcatcatttt gctggtttcg gcgcttttct gacgtaaaac actcgctaat
    18121 ggggaaataa atccgtaagc caataaaatg ccgaggaaag tccccaccat cgcatgtgcg
    18181 ataagcgcac ccagctcggc ggcaggacga tcggctgaac ctaacgcgtg aacgaccccc
    18241 attacagccg cgacaatacc aaacgccgga agtgagtccc cgaccagcgc cagactgttc
    18301 gccgggactt ctgcctcgct ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt
    18361 tcgaaggtgt tcatatgacc gctgataatc agacgcagat aatcgacgat aaaatcaagc
    18421 atgacgctat ccgcgaggat gcgtgggtag ctggcgaaga tctcgctctc acggggattt
    18481 tcaatatcac gttccagcga aaacatcccc atctgccgcg atttcgccat caaccgataa
    18541 agcagagcca gcagatccat atacattgct ttggtgtatt tggagcgacg aaacagcaac
    18601 ggcagcgcct tcagcgtgcc tttaatcgct ttgccattat tgccgacgat aaacgaccca
    18661 atccctgcac cggcaataat caccagttca gcgggttgat agagtgctcc aaggcttcca
    18721 ccggtcatca aataaccgcc gaaaactgta ccgagaacaa ccaggtaacc taataagata
    18781 agcacgacat catccttcca ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac
    18841 gcgcgattgg ggcaaaaaaa agcagcggta cgtcgttacc gctgctggaa tgttgcgcct
    18901 caccgtatca gttaaacagc ctgtactctc tgttcatcca gcagttgtgg gataatatcg
    18961 gcaggattct gggaaagttt acgtcttttt actgcccggg atggcggttg acataagctg
    19021 caggcaaagc tgccaacagg ctggtgagcg tgggtaataa aattgccgcc gcagcagttg
    19081 cagctggaaa gttgcagtaa tccactttca acaaaccgca ccaatgtcca ggcacgggtt
    19141 aatgccagca gtggtccttc ttctgcttgt gggcactgtt caaggtataa acggtaggct
    19201 ttgatcaccg catcgacgcc attacacaaa ccggttttca gtaaaaactg ccatgcatta
    19261 cagaacatcg aagcatgaac gttttgttcc caggtcataa accagtcggt tgagaatggc
    19321 agcatgcctt tcggcggtgg gcttccgcgc agttctttat aaagttttat caggcgtccg
    19381 cgacttaact gtgtttcgct ttccagcatc tgcaaacgag cgcccagggt gatcaattcc
    19441 attgccagct gaatatcccg cgcttcctga acaatgcttt tttcactcat gatcaggccc
    19501 ttttcttgcg cagcgcttct tcaggctgat taacatcatt cagcaagcgt gttgagagca
    19561 tgatgccggt atgaatttgc tggagatcgt caacgcggga atcttgcgtc aactgagtaa
    19621 tcgtctggtg gctgtcaaaa cggaagtgac aaaccagttg attggtttct gccagcttaa
    19681 ccatttgcgg aagagtcagt gccgctaacg ttgtcgccat ttcttcattt atgccgagac
    19741 gaaacatagc ggacgctttg tcctgaacaa tcaaacgctg tgcaagtagt aaatatgaca
    19801 agttgatgtc ataaatgtgt ttcagcaact cggaggtatg cattattccc acccagaata
    19861 accaacttta tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta
    19921 aaaaataatt agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg
    19981 tttaatatgt ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat
    20041 cgacgcaact gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat
    20101 aatgtgtgat gtagatcaca caaaacactc aattacttaa cataaatgta taagtcatac
    20161 ttttgttttg ggtgtatttc aatctgttaa aaagtttttc gctacgctag caagcaaaaa
    20221 tgaaacagga ataatcgaaa tgggatgttg cgcacagtta aaataactca ccgtaaataa
    20281 tcatctgcta taaataatca ctttcatgca ataccaga
//
LOCUS       DAZFDP010000080        20288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFDP010000080 DAZFDP010000000
VERSION     DAZFDP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..856
                     /locus_tag="ACHJ1G_002973"
     CDS             <1..856
                     /locus_tag="ACHJ1G_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4764790.1"
                     /translation="KNNVEHLLKSESTALRESICAVIAGKDCYQADTLSLLPGVKIIS
                     RFGTGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGY
                     WGESHGYELQGKRIGMVGYGNIGKTLAKRLSGFDVELLAYDKQPDYQVADKVGVQFVS
                     IEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIE
                     ERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGTHVAQSIIDYFA
                     GREIKNVL"
     gene            complement(936..1012)
                     /locus_tag="ACHJ1G_002974"
     tRNA            complement(936..1012)
                     /locus_tag="ACHJ1G_002974"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(976..978),aa:Met,seq:cat)
     gene            1221..2318
                     /gene="mltA"
                     /locus_tag="ACHJ1G_002975"
     CDS             1221..2318
                     /gene="mltA"
                     /locus_tag="ACHJ1G_002975"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4764791.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2395..3201
                     /gene="tcdA"
                     /locus_tag="ACHJ1G_002976"
     CDS             2395..3201
                     /gene="tcdA"
                     /locus_tag="ACHJ1G_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4764792.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3252..3695)
                     /gene="csdE"
                     /locus_tag="ACHJ1G_002977"
     CDS             complement(3252..3695)
                     /gene="csdE"
                     /locus_tag="ACHJ1G_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4764793.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(3695..4900)
                     /gene="csdA"
                     /locus_tag="ACHJ1G_002978"
     CDS             complement(3695..4900)
                     /gene="csdA"
                     /locus_tag="ACHJ1G_002978"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4764794.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5092..5319
                     /gene="ygdI"
                     /locus_tag="ACHJ1G_002979"
     CDS             5092..5319
                     /gene="ygdI"
                     /locus_tag="ACHJ1G_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4764795.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5670..6587
                     /gene="gcvA"
                     /locus_tag="ACHJ1G_002980"
     CDS             5670..6587
                     /gene="gcvA"
                     /locus_tag="ACHJ1G_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4764796.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6606..7001
                     /gene="ygdD"
                     /locus_tag="ACHJ1G_002981"
     CDS             6606..7001
                     /gene="ygdD"
                     /locus_tag="ACHJ1G_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4764797.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6994..8094
                     /gene="rlmM"
                     /locus_tag="ACHJ1G_002982"
     CDS             6994..8094
                     /gene="rlmM"
                     /locus_tag="ACHJ1G_002982"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4764798.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8138..8869)
                     /gene="fucR"
                     /locus_tag="ACHJ1G_002983"
     CDS             complement(8138..8869)
                     /gene="fucR"
                     /locus_tag="ACHJ1G_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4764799.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8927..9349)
                     /gene="fucU"
                     /locus_tag="ACHJ1G_002984"
     CDS             complement(8927..9349)
                     /gene="fucU"
                     /locus_tag="ACHJ1G_002984"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4764800.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9351..10769)
                     /gene="fucK"
                     /locus_tag="ACHJ1G_002985"
     CDS             complement(9351..10769)
                     /gene="fucK"
                     /locus_tag="ACHJ1G_002985"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4764801.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10878..12653)
                     /gene="fucI"
                     /locus_tag="ACHJ1G_002986"
     CDS             complement(10878..12653)
                     /gene="fucI"
                     /locus_tag="ACHJ1G_002986"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4764802.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(12686..14002)
                     /gene="fucP"
                     /locus_tag="ACHJ1G_002987"
     CDS             complement(12686..14002)
                     /gene="fucP"
                     /locus_tag="ACHJ1G_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4764803.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            14549..15196
                     /gene="fucA"
                     /locus_tag="ACHJ1G_002988"
     CDS             14549..15196
                     /gene="fucA"
                     /locus_tag="ACHJ1G_002988"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4764804.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            15224..16372
                     /gene="fucO"
                     /locus_tag="ACHJ1G_002989"
     CDS             15224..16372
                     /gene="fucO"
                     /locus_tag="ACHJ1G_002989"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4764805.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(16427..17182)
                     /gene="xni"
                     /locus_tag="ACHJ1G_002990"
     CDS             complement(16427..17182)
                     /gene="xni"
                     /locus_tag="ACHJ1G_002990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4764806.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(17294..18661)
                     /gene="sdaB"
                     /locus_tag="ACHJ1G_002991"
     CDS             complement(17294..18661)
                     /gene="sdaB"
                     /locus_tag="ACHJ1G_002991"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4764807.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(18719..20008)
                     /gene="sdaC"
                     /locus_tag="ACHJ1G_002992"
     CDS             complement(18719..20008)
                     /gene="sdaC"
                     /locus_tag="ACHJ1G_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4764808.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5007 a         4979 c         5345 g         4957 t
ORIGIN      
        1 taagaataat gtggagcatc tgcttaaatc agaatccact gcattacgag aatctatttg
       61 cgcggtcatc gccggaaaag attgttatca ggcggacacg ctatcattgc tgcctggtgt
      121 gaaaataata tcgagattcg ggacggggat agataatatt gatcttcgtg cagcacaaca
      181 atccggtatt gtagtcaata atgcggttgg gattaattca aatgctgtcg cggaatttat
      241 cattggactt atcttcgcga gcatgagaaa tattccaggc agctatcatg cgatgcaaaa
      301 tggctactgg ggtgagtcgc atggctatga attacaaggc aaacggattg gcatggttgg
      361 ctacggcaat ataggtaaaa ccctggcaaa aaggttatcc ggttttgatg ttgagctttt
      421 agcttatgac aaacaacccg attatcaggt tgctgacaaa gttggagttc agtttgtatc
      481 aattgaagat atctttatgc agtcgcatgt catcattgtt cttttgcctt tttctcctga
      541 gctggagaac ttcattagtc ataaatattt gtcgatgatg cgcaatggtg ctctaatcat
      601 caatgccgca aggggaaagt tgctggatga gggcgcttta ctacaggtga ttgaagaacg
      661 aaatgtgttt gcggcgctgg atgtgtttag ctctgaaccg ttggcgcaat ttagcccact
      721 gctacatgcc aaaaatatca ttacaacgcc gcatattgcg gccgccaccg ttgagtcata
      781 ccagcaaacc ggtacacacg ttgcccagtc gataattgat tacttcgcag gaagggagat
      841 aaaaaacgtg ttgtgagttc agcgaacgaa gtttttaaat acaaaaaaaa ccgcaatttg
      901 tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg gatttgaacc
      961 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgtccgtgga
     1021 tgcgcactat actccgctcg cgttttcatg caaccttttt tttaccccga tcacggattt
     1081 agcatgattt tgaacaaaaa caaaccacta aagcctattt tttgtgcaaa atttgcgtca
     1141 gggagaggct gctcatttca ttaaacgcgg cggtttgtta tcttcgttgc gccttatttt
     1201 ttaacctgaa gaagagaaca atgaaaggac gttgggtaaa gtaccttctt atgggcacgg
     1261 ttgtggcaat gcttgccgcc tgctcttcca aaccaaccga tcgcggacag caatataaag
     1321 acgggaaatt tacccagcct ttctctctgg tgaaccagcc agatgccgtt ggcgcgccga
     1381 ttaacgccgg tgattttgcc gagcaaatta accatatccg taattcgtca ccgcgtctgt
     1441 atggcaacca gagtaatgtt tataacgcgg tgcaagagtg gctgcgcgca ggcggtgata
     1501 cccgcaatat gcgccagttc ggcattgatg cctggcagat ggaaggtgcc gacaactatg
     1561 gtaacgtgca gtttacaggc tattacacgc cggtaattca ggcgcgccat acccgccagg
     1621 gcgagttcca gtatcctatt taccgtatgc cgccaaaacg tggtcgtctg ccgtctcgtg
     1681 cggagatcta cgcaggggcg ctgagtgata aatatattct cgcttacagc aactccctga
     1741 tggataactt cattatggat gtgcagggta gcgggtatat cgactttggt gatggcagtc
     1801 cgcttaactt tttcagctat gcagggaaaa acggtcatgc ctatcgcagc attggtaagg
     1861 tgctgatcga ccgtggcgaa gtgaaaaaag aagatatgtc gatgcaggcg attcgtcatt
     1921 ggggcgaaac acacagtgaa gccgaggttc gcgagctgct ggaacagaac ccgtctttcg
     1981 ttttctttaa accgcaatct tttgcaccgg tgaaaggggc aagtgcggtg ccgctggttg
     2041 gccgtgcttc tgttgcctcc gatcgttcaa ttattccgcc aggtactacc ttgctggcag
     2101 aagtgccgct gctggataat aacggcaaat ttaacggtca gtacgaattg cgtctgatgg
     2161 tggcgctgga tgtcggtggc gcaatcaaag gccaacactt cgatatctat caagggatcg
     2221 ggccggaagc cggacatcgc gcaggttggt acaaccacta tggacgtgtc tgggtgctga
     2281 aaaccgcccc gggcgcaggt aacgtcttta gcggctgatg tgttattctg tataccgttt
     2341 ttacggatac gacagggtga gggataacct cacccttttt aattctgagg tgctatgtct
     2401 gtggtaatta gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct gtatggtgaa
     2461 aaagcgttgc aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg tggtgtcggt
     2521 tcctgggcgg cggaagcgct ggcgcgcacg gggattggcg caatcacgct tatcgatatg
     2581 gatgatgtgt gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga taacgtcggg
     2641 ctggcaaaag cggaagtcat ggcggagcgt attcgccaga ttaacccgga gtgccgtgta
     2701 acggtggtgg atgatttcgt gacgccggat aacgtagcgc agtatatgag cgtggggtat
     2761 tcgtacgtga ttgatgccat tgatagtgta cggcccaaag cggcgctgat tgcttattgt
     2821 cggcgcaata aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat tgacccgacg
     2881 cagattcagg ttaccgatct ggcgaaaacg attcaggatc cgctggcggc gaagttgcgc
     2941 gagcgcctga aaagcgattt tggcgtagtg aaaaacagta aaggtaagct cggcgtcgat
     3001 tgcgtgtttt ctactgaagc gctggtgtac ccgcagtcag acggtactgt gtgtgcgatg
     3061 aaagccacgg cagaagggcc gaagcggatg gattgtgcat ctggatttgg cgcggcaacg
     3121 atggtgaccg ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa gatgatggcg
     3181 aaagcggcgc gtcagggtta aacaatcagg tcggatgcga cgcgaacgtc ttatccgacc
     3241 tggttctgtg cttaaacctg cttcgtcgca gcgataatcg cctcgcttaa cgcattcaac
     3301 ccctggctgc gtgaggcgct aagctgccca cgcaatccca gctcatcaaa caatgccagc
     3361 ggcgactgtg cctgcaattc ggcggcggtt ttcccctcaa cggcagtcaa cagcaccgcc
     3421 agcaggccgc gcactatacg tccttcgctg tcgccaaaga agtgcatttt accgttttca
     3481 gccacggtat atcccagcca gacgcggttt tcgcatccgg caatctcttt agcctgcgct
     3541 tttaactcgt ctggcaaagc cggaagctgt ttccccagca tgatcaactg gcgatattta
     3601 tcttcccatt gcgtcaacgg tgcgaaggta ttgcgtaacg tttctgcggt tacggttgtg
     3661 ccgaacggat gtccggcgaa ttgcgggttc gtcattaatc caccaataat tccagcgcgc
     3721 ggtcaacggc attcaccagc gcatccacat cactctttgt attatatggc gcaaaagagg
     3781 cgcgcagtgt gccggttacg cctaattctg ccagtagcgg ctgagcgcaa tgttgcccgg
     3841 cccgcaaggc aataccgtac tccgccagca gcgtcaccat atcgctgtga tgaacgccag
     3901 caaaatcaaa ggccagcagg ctggaatcct ggcagcggaa tgaacggaaa cctggacgtt
     3961 tcgccagcgc atcttccgcc agtgttgcta agctacggct ccagttttcg gcctggttga
     4021 tatcgtaatc tgccagccat tccagcgccg cgcttaatcc tatgacacca gccacatttg
     4081 gcgttcctgc ttccagtttc cacggcgcag attgagtcgt gaagccgtca aaactcactt
     4141 cgtgaaccat tttgccgccg cccagccagg gcgacatcgc ttccagcagt tctgatttgc
     4201 catacagcac gccgatacct gtcgggccat acagtttgtg acctgaaaaa gcatagaaat
     4261 caatattcag ttgctgaaca tccgcgggga aatgcaccgc cccctgagca ccatcaacca
     4321 tcaccaccat cccggctgaa tgagcaaagg taatcgctcg cgccagatcc gggcaaccgc
     4381 cagtaacgtt cgacatctga cccaacgcca gaatccgact acggggagta atcagttctg
     4441 gcaacaaatc gacatccggc agtcgctgcg cattaagcgg caatttcacc actttggctc
     4501 cagtttgttg ggcgaccatc agccagggga cgaggttggc gtggtgttct gccacgctga
     4561 caataatctc atcgcccagt tgcagacgcg gacgcgcata gcattgtgca accatgttga
     4621 tggattcagt ggtgccgcgc gtccagacga tagttttatc atccggtgca ttcagtaatt
     4681 gcgccacttt ctctcgtgca gcttcataac gcgcggtcag gcgttgggct tcggcaaact
     4741 ggctgcgatg gacgtttccg gcgctcaggc tgtaaaactg tcgggtggct tcaaccacgg
     4801 cttcaggttt aagcgcggtc gcggcgctgt cgagatagac gcccgcatcc tgtagagcgg
     4861 gaaactgggc gcgaaactgc gcgggattaa aaacgttcat ggtactcctc ggcttgatgc
     4921 accgatcgtg acgcaaaatt cactttgata caaggagagt gctaaccatc agaattatct
     4981 ggatatcctg gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc gttaatttta
     5041 gcctgttaat caatactatt aagatttatt aatcgcttta aggagaagat gatgaaaaag
     5101 actgccgcaa ttatttctgc ctgtatgctg acttttgccc tgagcgcctg ttccggttcg
     5161 aactatgtga tgcacaccaa tgacggacgt accatcgtct ctgacggcaa accacagact
     5221 gataacgata ccggtatgat ttcgtataaa gacgctaatg gcaacaaaca gcagatcaac
     5281 cgtactgacg tgaaagagat ggtcgaactg gatcagtaat tcacgatcgc aaggtaaaaa
     5341 aaagcaccgc aattaggcgg tgctacatta atcactatgg acagacaggg taaatgtaca
     5401 ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc gcagaccaat tgcaaacaca
     5461 acaacacaac atcacaaccg taagccaaaa gttcaccaga acacgcattc cgataaaact
     5521 tttcgttccg gctcaggaag tgccgccact ataggtattt gctggtagaa gctcaacgga
     5581 caatttataa tggctcagat taaaaaaact aataggttac acagtgcgat ctaattgtta
     5641 aattcattta acatcaaagt ttaaaagcca tgtctaaacg attaccaccg ctaaatgcct
     5701 tacgagtttt tgatgccgca gcacgccatt taagtttcac tcgcgcagca gaagagcttt
     5761 ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct tgaggatttt ttggggctaa
     5821 aactgttccg ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa agctatttcc
     5881 tcgatatcaa agagatattt tcgcaattaa ccgaagcgac gcgtaaactc caggcccgta
     5941 gcgccaaggg ggcgttgacg gtcagtttac tccccagttt cgccattcat tggttggttc
     6001 cgcgactttc cagctttaat tcagcttatc cgggaattga cgttcgaatc caggcggttg
     6061 atcgtcagga agataagctg gcggatgatg ttgatgtggc gatattttat ggtcggggca
     6121 actggccggg gctacgggtg gaaaaactgt acgccgaata tttattgccg gtgtgttcgc
     6181 cgctactgct gacaggcgaa aaacccttga agaccccgga agatctggct aaacatacgt
     6241 tattacatga tgcgtcacgc cgtgactggc agacatatac ccgacagttg gggttaaatc
     6301 atatcaacgt tcagcaaggg ccaattttta gtcatagcgc catggtgctg caagcggcta
     6361 ttcacgggca gggagtggcg ctggcaaata acgtgatggc gcaatctgaa attgaagccg
     6421 gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa aaacgctttt tatctggttt
     6481 gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg atcctggcga
     6541 aagccgctgc tgaacaagaa aaattccgct ttcgttatga acaataattt acgtagggta
     6601 cgaccatgac cagccgtttt atgctgattt tcgccgccat tagcggcttc atttttgtgg
     6661 ctctgggcgc ttttggcgcg catgtgttaa gtaaaaccat gggggccgtt gagatgggct
     6721 ggatccagac cggcctcgaa taccaggcgt ttcatacgct ggcgatctta ggtctggcgg
     6781 tggcaatgca gcgtcgcatc agtatctggt tttactggag tagcgttttc ctcgcgttag
     6841 gcacggtgct gttcagcggc agcctttatt gcctggcact gtcccatctg cgtttgtggg
     6901 cgtttgtcac tccggttggc ggcgtgagct tccttgcggg ctgggcgtta atgttagttg
     6961 gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt gctgtgccgt
     7021 ccgggctttg aaaaagagtg cgccgcagaa attaccgata aagccggcca gcgggaaatt
     7081 ttcggttttg cccgcgtgaa agagaatgcg ggttatgtca tttatgaatg ttatcaacct
     7141 gatgatggcg ataagttaat ccgtgagctg ccgttcagtt cattaatttt tgcccgccag
     7201 tggtttgtgg tgggggaact cctgcagcat ttgccgccag aagatcgtat tacccccatt
     7261 gtcggcatgt tacagggcgt agtagagaag ggcggtgaac tgcgtgttga agttgccgat
     7321 accaacgaaa gcaaagagtt gctgaaattc tgccgtaaat tcaccgtgcc gctacgcgct
     7381 gccttgcgcg atgccggggt gctggcgaac tatgaaacgc cgaagcgtcc ggttgtgcat
     7441 gtattcttca ttgcaccagg ttgctgctat accggttact catacagcaa caataattcg
     7501 ccgttctata tgggcattcc gcgcctgaaa tttccggcag atgcgccgag tcgttccaca
     7561 ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg atgagtggga tgaacgcctg
     7621 gcgaacggga tgtgggcggt ggatttgggc gcttgcccag gcggctggac ctaccaactg
     7681 gtgaagcgca acatgtgggt ttattccgtc gacaacggcc cgatggcgca aagtctgatg
     7741 gataccgggc aggtgacgtg gctgcgggaa gacggtttca aattccgtcc gacacgcagc
     7801 aatatctcct ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc ggcattgatg
     7861 gcgcagtggc tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa actgccgatg
     7921 aaaaaacgct acgaagaagt atcacacaat ctggcgtata ttcaggcaca gcttgatgaa
     7981 catggcataa atgctcagat tcaggcgcgg cagttgtatc acgatcgcga agaagtgacg
     8041 gtgcacgtcc gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg ataacaggca
     8101 aggccggggc gtcgtctccg gcctgctacc ctttccgtca ggctgttacc aaagaagttg
     8161 caacctggcg ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg atgcgggctt
     8221 cccctgaacg attaaattta cttttatcaa tcaataacaa cgattgcgcg gcacgtttta
     8281 atagcatcga tttgtaatca gcgttgatgg cattggagtc ccacagtgcg ccgctgctat
     8341 cgatcccttc acaagaaaaa ataaacagat cgatttccag cgatttaagt tgggaaatca
     8401 gcgagggatt gacgtaacag ccatatttgc gctcaagtgt gccgccggaa ctgatcagtt
     8461 gaatgcgttc gcgtttaccg agttcatggc aaatcggatg gctattggtg aagacctgaa
     8521 tgttgatgtc aggcaactgg cgtgccagat accagcaagt tgaactggcg tctaaggcta
     8581 tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct ttatgcgcat
     8641 aatggctttt cagccggatg tgaaagggat cgccactgtc ttgattttga cggtggatat
     8701 atttagcgcg tccatgattg cgcagaattt taccctgcgt ctgtaattca ttgagatcgc
     8761 gacgaatggt ttctttactg acctttagct gttcagagag agcttccgtg gtcaggctgg
     8821 tatggttcag cagcaggtcg actatcgctt gctggcgtgc cgctttcata tttttcaccc
     8881 catcattcga taaccgcccg cgtcatgcgg gcgtaccggt atgagattac ggtgttaccc
     8941 ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg atcgcaaagg
     9001 ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg gatgatgtca ggacacgggg
     9061 cttgtagtga aagcgcatta cggtaacgtc gttctacttc aggatcgaga gtgtcacctt
     9121 ctaccgctgc catcatcacc agcggcgggg cataactgtc cagctcaaat aacgggataa
     9181 tcgcctggag caagtcgctc accaacaggc catcagcgcg gatcacctgc ggtcccatcg
     9241 aatgggcggg aaagtgagca tcggaaaaaa taatttcatc tccatgtccc atctctgcca
     9301 gcactttcaa tagttcggga gaaattaacg gcgaaattgt tttcagcatt tcacacttcc
     9361 tctataaatt caggttcagt ttgcgggtag aaataacggt actgataatg aatctgtgca
     9421 cgggcttctt ccgggctgtt gaattcccct acgccatacc aaccgaacag cgcagctcct
     9481 gcgacggtcg tttcggcatc gtcgagaact tttaccggaa tatcaagcat attggcttta
     9541 atctgattcc acaatgtgtt gcgacttcct ccaccgacta acaataattc agaggcctta
     9601 aagtgcccga ttttttccag catctgtaga ttgcgctgta attgcgcagt taacccttcc
     9661 agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg tcactccttg ccagccagcg
     9721 ttctgacacg acaataaatc acactgcatt tttacgccat ccgcgccagg cgcgatcaga
     9781 cgagcttctt caatcaacat ttgccagggt gtttcagccg tccagaacag ttttctcacc
     9841 cattccagca cgccggatgc cagccattgc atacctgggt tatacaaccc tgcctggcta
     9901 tccagttcgc aggtggaacc ggcgtactga cttaacagcg aagtatcaac ctgggcgcta
     9961 cggaccatta aaatttccca tgtaccggaa gagagcacgg gttcattttg gtcggcacca
    10021 gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat gccaacgggt
    10081 aagccgagca ttgctgcggc gctgttctgt agcgtaccaa tctgttcacc cgcttccacc
    10141 agacgaggga agagtcggcg tggaataccg gtggcttgta aaatttgcgg actgaaatcg
    10201 cgttgctgga tatccagcat ctggctggtt ccggccatcg tgatatcagt agtgaattcg
    10261 ccggttaaac ggtggttaat cagcgacgaa ataaagagcc aggcgtgcgc gcgttccagc
    10321 agttgtggat gattttcttt caaccacacc aacttatata acgtattgaa actaaaggct
    10381 ccgacgccag aaatagcctg caaccgctgt gcggagatca actgttcaat attgtccata
    10441 accgctgctg ttcgcggaca tttccagcta atgatcggat agagcagatt gccttgctta
    10501 tctaccagag cgccatccac accaaaggtg gtgacggcga taccgcggat gtggcattca
    10561 gtcagttcac tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat ggcgtctaaa
    10621 gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg cgttgaggcg
    10681 cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt cgcgccacag
    10741 tcgagtacca ggataacttc ttgtttcata atcgctcctg caatatagcc ggataacatt
    10801 gcttatccgg ctaaccactc ttgcttgttc aggcccggta gccggagcga ccgggcatca
    10861 catcagggag taatgtatta acgcttgtac aacgggccgt agttctggca agcgcggtaa
    10921 tcctggcctt caatatccat gccgtgcgca gcccaggcag aaggacgata cactttggtc
    10981 tcttcaacgt tgtgcataca taccgggata cgcagcatgg aggcgagagt gataaagtct
    11041 gcgccaacgt ggccgatggt cagaacccca tggttagcgc cccagttcgc cattaccgag
    11101 tacacatccg taaacggccc tttaccggtg aggcgcggtg caaaccaggt ggttggccag
    11161 gttgagttgg tgcgtttgtt gaggatgtca tgcacatcct tcggcaattc cacgctccag
    11221 ccttccgcga tttgcagtac cggtcccagg cctttgatga tgttgacacg ggtcatggtg
    11281 aacgggacgc cgccttcggt aaggaagcgg gaagagtaac cgccgccacg gaagtattcg
    11341 tggatcgccg ggcaccattc ggtagcggcg aggcaagcgt cagcctcttg ctgagagatt
    11401 tcccagtgcg gcttcatcgt cgggttacct tcgctgtcgc gttgtttaca ggaaccgtcc
    11461 agcgcagcag aaccggagtt gatcaaatgg atgatgccgt gttctgccag tccatcgagt
    11521 ttatgccccg ttacacgctc aattgcttct ggtgaccagt aggtacgcac atcggcaaat
    11581 acctgagcgg tgccggtgag ctggtgaccc attagcattg ccacgccgtt aagactgtcg
    11641 ttttcggtcg ccacgacaaa gggttcgcgc acgccattcc agtcaaatga actgttaagg
    11701 atcgcttcgg cggtgtcacc attgggatat tgatcggtcc agtgacgttg cccctggaag
    11761 cccgcagcga tggcgttgta gccaagtgat tcttccacgc gaccaatatc ggccagtttg
    11821 ctgttgcctt gcatcatgtc gcggatacac atcgccatca gtaaactttc gcgcagaact
    11881 gcgcggcgtt gctcggcatt gcgttgatac tgtttgttat tttcatcttc gccatagcgg
    11941 aagtttttat cagcccaggc cagtgccatt tccagttcgg cttcgtcgta aatcttctga
    12001 tcgatacggc gacgcagttc ggtcatatcc accgcctgga ctttcattcc cagccaggat
    12061 tcaaagaagt tgtgatcaac aatggaaccg gcgataccca tcgaaacgcc gcccagcgac
    12121 agatagcttt tacctttcat gctggcgacg gccaaaccgg cgcgggcaaa gcgcagcagt
    12181 ttttcttcaa catcggcagg aatcgatgtg tcatcggcat cctgaacgtc atgaccgtaa
    12241 atggagaatg ccgggatgcc tttctggctg tgagctgcca gagccgctgc caggtaaaca
    12301 gcgccgggac gttcagtgcc gttaaagccc caaatggctt tcgggcgggt tggatccatg
    12361 tcgatggttt cactgccata gcaccagcaa ggcgttaccg taatggtgag gcctacattc
    12421 tgactgctga atttttcttc gcaagcagcg gcttcagcca tacccgcgat acaggtatcg
    12481 gaaatgacac actcgacggc agctccgcag gcatggcgca gtttctcggt cagcagtgcg
    12541 gccgtagctt tcgccatatt cattgtttgt tcttcaagcg actcacgaac acccatgcga
    12601 cgaccgtcaa taaccgggcg gataccaatt ttcggtaagc tgattttttt cattacagat
    12661 tcctcacttt attcggaaaa tatgttcagt tagttgccgt ttgagagcgg aaacgggcaa
    12721 agataaagat gaccgcgaag cagagtgcgg ggatcagttc agcagtgggg atgttgcccg
    12781 ccgcgtcact gacaaaaccc atgaccggag tgacaatacc gccgccaatg atggtcataa
    12841 cgatgaagga cgaaccgtat ttggtgtcct ggccgagatt cttaatgccc agcgagaaga
    12901 ttgttgggta ctgaatcgac ataaaggcgc tgcataaagt cagggctatt aagcccacat
    12961 gaccgccagc gaaggctgag atcaggcaca gtgccatagc gattaatgcg taggcggcca
    13021 ggactttgtg tggtgcgaag cgactgatga gccaggtacc ggtgaaacga ccaataaaga
    13081 agcacaccat ggttccggtt aaatagttag cggcaaagcc tgcagtcata cctggaattt
    13141 cttctacagc gtagcgaatc aaatagctcc agcaggccgt ttgtgcgcca acgtagcaga
    13201 attgcgctaa tactgcccag cgccagtggc gaatacgcgc caggcgagaa agcgatgcgg
    13261 agaacgatcc ttgtttggcg tcactgtgat tatcactctg caatgccggg aatttcgtca
    13321 gcatgatcag cagggcgacc agtaacacga tagccacgat gatcatataa ggtgtctgta
    13381 ccgataatac caggctgtgt ttatacgcac tcaattgctc tggagacatt ttatcgagaa
    13441 cgtcttgcga ttgatgtggc acgttagaca aaataagact ttgcccaaag acaaccgcga
    13501 taattgcgcc aaacgagtta aatgtttgcg caagatttaa gcggaagtga ccactgcttt
    13561 ccggccctaa taccgtaaca aaagggtttg cggcagtttc cagacaacct aatccggctg
    13621 caataataaa taggccaact aaaaataagg tgtagttcat tatttctgca gcgggccaga
    13681 ataatgcagc gcccaaggca tataaaaata atccggtaat aatccctgct ttataactga
    13741 gttttttcat caatatccca gcagggattg ggataatgaa ataaccaaag taaaaggccg
    13801 attggatcag gccagcctgg aaatttgtca gcgtaaaagc ctgctggaat tgaggtaata
    13861 aaatgtcgtt aaggttattg gctaccgccc aaagaaaaaa cagtgagcac agcagcgcga
    13921 atggaataat gtaacttctg ctttgccctg catctttatc taccgcacgg taactctgcg
    13981 tttgtattga tgtgtttccc atagcatcct cttaggttca gaagcttaag cacgtaatcg
    14041 gttatgtttt caaaagtgca aaaccagcga gttcgggctt catatcagtg aatatttgcc
    14101 tgtctgaaat gaactcatgg attcattttc aaataaaaaa cgctctgtaa tatgacggcg
    14161 gtcacactta ttagctggaa ggtgacttta tgtgactacc atcactttaa taatcgaaat
    14221 ttcatgcccg tttcagaaaa taaatcataa aaaacacaaa ccagatgacc gtattaattg
    14281 tttatgccga aaacgggcat tcctggcttg agtgaataga aaaatgaccg ttttgataaa
    14341 tatttttaag agcggtcatt tttaatgggg ttataaaaag agaacttgca gttatttact
    14401 gtgattagtt gaaccaggtc acaaaatata agcttgtgaa tttctttctg gttaatcagg
    14461 aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca acattattgc
    14521 cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc gtcagattat
    14581 tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg ggaacgtcag
    14641 tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg aaaaactgac
    14701 ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa agctcccctc
    14761 aagcgaatgg cgtttccata tggcggccta tcaaagcaga ccggatgcca acgcggttgt
    14821 tcacaatcat gccgttcatt gcacggcagt ttccattctt aaccgatcga tccccgctat
    14881 tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt atgcgacctt
    14941 tggaacacgt gaactttctg aacatgttgc gcaggctctc aaaaatcgta aggcaacttt
    15001 gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt tatggctggc
    15061 gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta cggacccggt
    15121 gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa cctatgggtt
    15181 acgaattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta acagaatgat
    15241 tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg atgaggtgaa
    15301 acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc aatgcggcgt
    15361 ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga tttacgacgg
    15421 cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat tccagaatag
    15481 cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt gtaaagcgat
    15541 tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag gactttcccc
    15601 gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca ctgcggcaga
    15661 agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg tttgcgttga
    15721 tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg gtatgcctcc
    15781 agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg ggtatattac
    15841 ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa tcattgctgg
    15901 ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg cgctcgggca
    15961 gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg gtatggcgca
    16021 tccactgggc gcgttttata acactccaca cggtgttgca aacgccatcc tgctaccgca
    16081 tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg cgcgcgttat
    16141 gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg ttgaagcggt
    16201 gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg gggtacgcaa
    16261 ggaagacatt ccggcactgg cgcaggcggc actggatgat gtttgtaccg gtggcaaccc
    16321 gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt aaatgcgctg
    16381 atgtgataat gccggatacg acgtttgcgg cgtatccggc tcgccgttac cgtaccaacc
    16441 gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca atatcgcggc
    16501 acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg gcaaccgcat
    16561 ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc gtggcgcttt
    16621 ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca agtccccagt
    16681 aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc gcatccagcc
    16741 aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga cagtagcctt
    16801 tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc gccagcgtgg
    16861 cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg cgttgctcaa
    16921 aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc ggtcgacccg
    16981 ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca tcatcaaaaa
    17041 cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca tgctggcagg
    17101 tttcgacaca gggcgatccc tgaacggcat gaatgcgacg aataagattc agtgcatcga
    17161 caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa gggtaacatt
    17221 cgatgccgga acaggctacg gctggatgag aaatcgggaa gaggcctcgc aaaacgaggc
    17281 ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg cgagaggttt
    17341 cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata actttatcga
    17401 ggcagacgcg cggctcgctg gtacggcgta gcgccatacg tgcggcgttc accgctttta
    17461 ctgccgcaat ggcgttacgc tcgatgcatg gcacctggac ctgtccggcg accgggtcac
    17521 acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc tgcgccgggc
    17581 ttgcacctaa cagttctgcc agaccagccg ccgccattga gcacgccaca ccaacttcac
    17641 cctggcagcc gacttcagca ccagaaattg acgcgttcat cttataaaga gaaccaatgg
    17701 cactggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg ataaacttgt
    17761 cgtagtacgc cagcactgcc gggataatcc cgcacgcacc gttagtcggc gcagtcacca
    17821 cgcgtccgcc agcagcgttc tcttcgttca ctgccaatgc aaacatgttg atccagtcaa
    17881 caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg cgtagtgccg
    17941 cagcacggcg tggaacgcgc agtttgccag gcaacacgcc ttcggtggaa ataccgcgct
    18001 caataccgcc acgcatgact tcccatacgt tcgccaggtg ctgttccagc tcttctttgc
    18061 tgtgcagcgc cagctcattt ttcatcatca ggccagagag tgacagcccg gtttcctgac
    18121 aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc ggtgcgctat
    18181 tctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag tagtaagtct
    18241 ggctgtaaac gactttatcg cccgccagcg cggtaatgcg cataccgttt tcatgcagag
    18301 aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg gaactccact tcatgctgac
    18361 cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca gggatggaat
    18421 cgatatccac ggtatccggc aggttacccg ccaggcccat aataatggcg atatcagtgt
    18481 ggtggccttt accagtcaga gagagcgagc cgtacacgtc aaccaccacg cgggtcacgt
    18541 ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct ttcattggtc
    18601 caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat acgctaatca
    18661 taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc gaaaggactt
    18721 agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg acaacgaata
    18781 cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt gcgtacatcg
    18841 gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc atacccagga
    18901 tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc gcagtgatac
    18961 ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta atcaccatac
    19021 cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg atagcgataa
    19081 tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta gctaggtaag
    19141 acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc aggctcagta
    19201 cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg aatgccagga
    19261 tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca acggcgaaag
    19321 aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc agccacaggg
    19381 tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt tccagtgctg
    19441 cgccgttcca ctgcgggatc aggtacagag ccagcagcat cagtacgcca acaaacggga
    19501 ataccagaat actcatcgct ttaacgatca tctgctcacc aaagcgaacg atggtcatca
    19561 tacccacgat caggatcagc gacagaatcg cacgcggcgg tggcgtcata cccagctggt
    19621 gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc aggatcgggt
    19681 agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca aagtgttctt
    19741 ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga cagtacgaag cgagtcaggc
    19801 cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc agcgggatca
    19861 taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt gccgtgccgt
    19921 aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag tctttcgacg
    19981 caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca aataattcag
    20041 cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct gtctatcggc
    20101 gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac acaagttatg
    20161 tatctatcag tgatcttgat cccaaataca ataatctact tggaagatag attatcccaa
    20221 cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg gcaatatttg cccattaaac
    20281 gacatctg
//
LOCUS       DAZFDP010000081        19869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFDP010000081 DAZFDP010000000
VERSION     DAZFDP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2461
                     /locus_tag="ACHJ1G_002993"
     CDS             <1..2461
                     /locus_tag="ACHJ1G_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO4764809.1"
                     /translation="KLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTISGPAF
                     NEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVLVAF
                     VLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYLLK
                     EETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHLL
                     NTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQ
                     EAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKM
                     ISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLE
                     PLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADG
                     MRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLL
                     EQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLN
                     DIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGR
                     INKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLG
                     TLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLA
                     ELVTKGHLRPLKEASEAENVA"
     gene            2500..2682
                     /locus_tag="ACHJ1G_002994"
                     /pseudo
     CDS             2500..2682
                     /locus_tag="ACHJ1G_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(2795..3631)
                     /locus_tag="ACHJ1G_002995"
     CDS             complement(2795..3631)
                     /locus_tag="ACHJ1G_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO4764810.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3631..4434)
                     /locus_tag="ACHJ1G_002996"
                     /note="aph(3'')-Ib"
     CDS             complement(3631..4434)
                     /locus_tag="ACHJ1G_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO4764811.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(4495..5310)
                     /gene="sul2"
                     /locus_tag="ACHJ1G_002997"
     CDS             complement(4495..5310)
                     /gene="sul2"
                     /locus_tag="ACHJ1G_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO4764812.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5647..5850
                     /locus_tag="ACHJ1G_002998"
     CDS             5647..5850
                     /locus_tag="ACHJ1G_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764813.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
     gene            5841..6062
                     /locus_tag="ACHJ1G_002999"
     CDS             5841..6062
                     /locus_tag="ACHJ1G_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764814.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(6297..7283)
                     /gene="mobV"
                     /locus_tag="ACHJ1G_003000"
     CDS             complement(6297..7283)
                     /gene="mobV"
                     /locus_tag="ACHJ1G_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFO4764815.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHL
                     AARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWL
                     ADKYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAER
                     VADLGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELNPQKVKGVSLAEKVFG
                     AVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPF
                     KGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
     gene            complement(8753..9088)
                     /locus_tag="ACHJ1G_003001"
     CDS             complement(8753..9088)
                     /locus_tag="ACHJ1G_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764816.1"
                     /translation="MRTITTREQLLVNGKVRERIATHIVTGAHGYETLCTSGYNLQYN
                     KERVLIENCEKVADGELPVTCHTCFSIWQDVHRFKPGDFDTESGKGNFTDTELTKITI
                     GQEKTPNAC"
     gene            9426..10406
                     /locus_tag="ACHJ1G_003002"
     CDS             9426..10406
                     /locus_tag="ACHJ1G_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4764817.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATVPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRTVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQRERILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            10409..10831
                     /locus_tag="ACHJ1G_003003"
     CDS             10409..10831
                     /locus_tag="ACHJ1G_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4764818.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKTILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            10855..11145
                     /locus_tag="ACHJ1G_003004"
     CDS             10855..11145
                     /locus_tag="ACHJ1G_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764819.1"
                     /translation="MLRPFFFSDKECRWAPLFTAPADAARQERCLWSVSRVRRARDGR
                     FYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            11255..12157
                     /locus_tag="ACHJ1G_003005"
     CDS             11255..12157
                     /locus_tag="ACHJ1G_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4764820.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVTHYGG"
     gene            12154..12465
                     /locus_tag="ACHJ1G_003006"
     CDS             12154..12465
                     /locus_tag="ACHJ1G_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764821.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            12542..13225
                     /locus_tag="ACHJ1G_003007"
     CDS             12542..13225
                     /locus_tag="ACHJ1G_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4764822.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFGVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            13226..13447
                     /locus_tag="ACHJ1G_003008"
     CDS             13226..13447
                     /locus_tag="ACHJ1G_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764823.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            13377..13895
                     /locus_tag="ACHJ1G_003009"
     CDS             13377..13895
                     /locus_tag="ACHJ1G_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4764824.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQY
                     TENTPLMLVVWSPEEIQALADGMEISLTGHEIRTVLARLEDIPEEQRIESGISSAAVM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQA
                     RTLLKNNTHEND"
     gene            13940..14710
                     /locus_tag="ACHJ1G_003010"
     CDS             13940..14710
                     /locus_tag="ACHJ1G_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764825.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWVSIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEYDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVLEARSEIRSMADALAKIRG"
     gene            15123..15548
                     /locus_tag="ACHJ1G_003011"
     CDS             15123..15548
                     /locus_tag="ACHJ1G_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4764826.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            15595..16017
                     /locus_tag="ACHJ1G_003012"
     CDS             15595..16017
                     /locus_tag="ACHJ1G_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4764827.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            16014..16205
                     /locus_tag="ACHJ1G_003013"
     CDS             16014..16205
                     /locus_tag="ACHJ1G_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764828.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIIDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(16484..16732)
                     /locus_tag="ACHJ1G_003014"
     CDS             complement(16484..16732)
                     /locus_tag="ACHJ1G_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764829.1"
                     /translation="MSRGPLQRRRARARNGVAPYIPEAFCRDAGSAGHGATEAARPER
                     SLQAGGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            17247..17477
                     /locus_tag="ACHJ1G_003015"
     CDS             17247..17477
                     /locus_tag="ACHJ1G_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764830.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            17529..18890
                     /locus_tag="ACHJ1G_003016"
     CDS             17529..18890
                     /locus_tag="ACHJ1G_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4764831.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     DGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            18937..19500
                     /locus_tag="ACHJ1G_003017"
     CDS             18937..19500
                     /locus_tag="ACHJ1G_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4764832.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFIHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDIREELPRGTFAYTDVPTMIIRLRA"
     gene            19625..>19869
                     /locus_tag="ACHJ1G_003018"
     CDS             19625..>19869
                     /locus_tag="ACHJ1G_003018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764833.1"
                     /translation="MLLSFHACHLSVTDDVVVPATATATATATATATATALLFPAALI
                     LTTAVLSCLPGAPSAPGCLAASGRQARLRQAAAGAPA"
BASE COUNT         4397 a         5324 c         5665 g         4483 t
ORIGIN      
        1 caaactggca ctgataccgt cagccgaaca gcgttcacag ctggagatgc tgctggggcc
       61 aactgattgc agccgcctgt ctttactgga atcactgaaa aagggccctg tgaccatcag
      121 tggtccggcg tttaatgaag caattgaacg ctggaaaact ctgaacgatt ttggcctgca
      181 tgctgaaaac ctgagtacac tcccggctgt gcgcctgaaa aatctcgcac gttatgctgg
      241 tatgacttcg gtgttcaata ttgccaggat gtcaccgcag aaaaggatgg cggttctggt
      301 tgcctttgtc cttgcatggg aaacgctggc gctggatgat gcattggacg ttctggacgc
      361 catgctggcc gttatcatcc gtgacgccag aaagattggg cagaaaaaac ggctccgctc
      421 gctgaaggat ctggataaat ctgcattggc gctcgccagc gcatgttcgt acctgctgaa
      481 agaagaaaca ccggacgaat cgattcgtgc tgaggtgttc agctacatcc caaggcaaaa
      541 gctggctgaa atcatcacgc ttgtccgtga aattgcccgg ccctcagacg ataattttca
      601 tgaagaaatg gtggagcagt acgggcgcgt tcgtcgtttc ctgccccatc tgctgaatac
      661 cgttaaattt tcatccgcac ctgccggggt taccactctg aatgcctgtg actacctcag
      721 ccgggagttc agctcacggc ggcagttttt tgacgacgca ccaacggaaa ttatcagtcg
      781 gtcatggaaa cggctggtga ttaacaagga aaaacatatc acccgcaggg gatacacgct
      841 ctgctttctc agtaaactgc aggatagtct gaggcggagg gatgtctacg ttaccggcag
      901 taaccggtgg ggagatcctc gtgcaagatt actacagggt gctgactggc aggcaaaccg
      961 gattaaggtt tatcgttctt tggggcaccc gacagacccg caggaagcaa taaaatctct
     1021 gggtcatcag cttgatagtc gttacagaca ggttgctgca cgtctttgcg aaaatgaggc
     1081 tgtcgaactc gatgtttctg gcccgaagcc ccggttgaca atttctcccc tcgccagtct
     1141 tgatgagccg gacagtctga aacgactgag caaaatgatc agtgatctac tccctccggt
     1201 ggatttaacg gagttgctgc tcgaaattaa cgcccatacc ggatttgctg atgagttttt
     1261 ccatgctagt gaagccagtg ccagagttga tgatctgccc gtcagcatca gcgccgtgct
     1321 gatggctgaa gcctgcaata tcggtctgga accactgatc agatcaaatg ttcctgcact
     1381 gacccgacac cggctgaact ggacaaaagc gaactatctg cgggctgaaa ctatcaccag
     1441 cgctaatgcc agactggttg attttcaggc aacgctgcca ctggcacaga tatggggtgg
     1501 aggagaagtg gcatctgcag atggaatgcg ctttgttacg ccagtcagaa caatcaatgc
     1561 cggaccgaac cgcaaatact ttggtaataa cagagggatc acctggtaca actttgtgtc
     1621 cgatcagtat tccggctttc atggcatcgt tataccgggg acgctgaggg actctatctt
     1681 tgtgctggaa ggtcttctgg aacaggagac cgggctgaat ccaaccgaaa ttatgaccga
     1741 tacagcaggt gccagcgaac ttgtctttgg ccttttctgg ctgctgggat accagttttc
     1801 tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg atgccgacta
     1861 tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag tccttcagtg
     1921 ggacgaaatg atccggaccg ctggctccct gaagctgggc aaagtacagg tttcagtgct
     1981 ggtccgttca ttgctgaaaa gtgaacgtcc ttccggactg actcaggcaa tcattgaagt
     2041 ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg
     2101 gcgcattctg acccagctta atcggggaga aagtcgccat gccgttgcca gagccatctg
     2161 tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcaactggg
     2221 cacactgggg ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc
     2281 cctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacgcctctc
     2341 cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact
     2401 ggtgaccaaa ggacatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta
     2461 acgtgagttt tcgttccact gagcgtcaga ccccttctac gggttcgctg cgagtcttgc
     2521 cacgccgagc ccctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg
     2581 gcgcggcgga tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc
     2641 atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc
     2701 gggaaggggc ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat
     2761 gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct
     2821 tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg
     2881 cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg
     2941 cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc
     3001 tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga
     3061 cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc
     3121 catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt
     3181 ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg
     3241 caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca
     3301 gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga
     3361 tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga
     3421 gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat
     3481 cagcaatgtc ttttataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg
     3541 aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc
     3601 aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag
     3661 atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc
     3721 gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag
     3781 tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca
     3841 ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac
     3901 catatcggtg cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag
     3961 atcgagctgc ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg
     4021 ggacaccaca tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg
     4081 atcaaccgat aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag
     4141 caaatccgct ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca
     4201 tgcaccctcc tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt
     4261 gagccaaatg aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat
     4321 tttcgcgaag gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct
     4381 gacaggcaac cagtcagaat gcgattcacc aaaaaaaata ttagttcgat tcaatggagg
     4441 ttccttcagt tttctgatga agcgcgaata tagagaaata tcccgaatgt gcagttaacg
     4501 aattcttgcg gtttctttca gcgccgccaa taccgccagc ccgtcgcgca aggggcgcgg
     4561 ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg gcaagctctg cagcgagtgt
     4621 cgcggccccg acatcccccg gaccacggcc tgtgagcgcg cgcagaaagg atttgcgcga
     4681 aacagacaga agcaccggca aatcgaagcg cagccgcaat tcatcgaacc gcgccagcac
     4741 cgagagcgag gtttcgggag cagcccccag aaaaaacccc atgccgggat caaggacaag
     4801 gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg cgcgcgtcaa agaacgccgc
     4861 aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga tctgcctgcc cgtcttgcac
     4921 cgaatgcata acgacgagtt tggcagatga tttcgccaat tgcggataga acgcagcgtc
     4981 tggaaaaccg cgaatatcat tgagataggc cacaccacgc gacaaggcat aggcttgcgt
     5041 cgcgggttga taactgtcga gcgagacggg aatgccatct gccttgagcg cgtccagcac
     5101 cggcgcgata cgcgcgattt ctgtgtcgga cgaaacaggc gcggcgtcgg gattgctgga
     5161 tgccggaccg aggtcgatca catctgcccc ctcggccatc agcttacgcg cctgcgcaat
     5221 ggctgcgtct ggcgccagat accggcctcc atcggagaaa ctgtccgagg ttatgttgac
     5281 gatgccgaaa atgatgagcg atttattcat gggggcttct ataataataa taatcgagca
     5341 tgagtatcat acggatgctc gggtcgaaag ggaatcccca ggcgagtaac ctgtttgcgg
     5401 tgatccatta gctgcaggag cagaagagca tacatctgga agcaaagcca ggaaagcggc
     5461 ctatggagct gtgcggcagc gctcagtagg caatttttca aaatattgtt aagccttttc
     5521 tgagcatggt atttttcatg gtattaccaa ttagcaggaa aataagccat tgaatataaa
     5581 agataaaaat gtcttgttta caatagagtg ggatacagac acgcttgata tgtttggaga
     5641 tcagccatga cagatccact caaagccctt ttcggcaagc ctgattactc ccatatcgta
     5701 agggacacca cggccactat cagcatcaca gccgctgaaa tggccgctgt tttggaggct
     5761 tacgacaggg gtatagacac gctggacggc accaccagaa cggcgctgga ttccgttata
     5821 tcgaaactca aggacgaggt atggccatga gcatgtatta catcaagaca ggtctggagt
     5881 actggaaacc caagggctac gggtacacca agagccgcga ggaggcgggg gttttcaccc
     5941 tcaaggacat ggaagaattg aacctcgatg gctgcaccct ggaacgcgcc gaagtggcgt
     6001 tcctggacga ggacggcccc agtcccgacc taccccaccg cgtcaccctg ccccgcgatt
     6061 gagcggggcg ctggagggtt tcaaaggggc accctttggc acgggatatg tgtcaaaaac
     6121 ggagtttttg taaacatatc cgacgtgcct gtaaatccgc gcgattactg ccagccctta
     6181 caccccggtt gcttagggtt ggttgaaagt ggtttagggt tggttgaaag tggtttaatg
     6241 ttggttaaac aatggtttaa ggttgttcaa ggttggttaa agttggttga aaagggttaa
     6301 cggcccctgc tgcggtcgct gcgctcccgt tcccgcttgg ccttgaatcg ctctttgatc
     6361 tgctgggcgc gctcctgctt ctcctgctcg ttctcctgcc gcaatttcac cgcctgccgg
     6421 atcagtcccg ctacctgatc cttggatagc cccttaaacg gctcctgaag ggcctgtaag
     6481 cgtttttgct gctgggctag ggtttccctt agctccttgg ctcttcgctc gttctgggcg
     6541 cttacagcgg ctttctcggc catcggctgc acagacccca ttattttggc gttcaacctt
     6601 tgcgccaccc cttcgaccgt ctccacggct ccgaacacct tctcggccag tgacaccccc
     6661 ttgaccttct gggggttcag ctcgtcaggc gtcaggtgtg gcacgtctcg gcctgcctgc
     6721 tggatcgccc catagtgggt tttaactcgc tggtgtgtgg ccctgctgcc ctcgatgccc
     6781 cgttcaagcc ccagatcggc cacccgttcg gcgtagctgg tctggtcctg gctcatcttc
     6841 gtccggttgc caatgaactc cttggcactc aggcgcccgt cagccgtccg aggcaccacg
     6901 aacgccgaca ggtgcggggt cagctcatcc cggtggatcg tggccgtgac cacgttctca
     6961 gcgccgtact tgtcggccag ccactcggtc gatcgcttga agaactcccg ctgctggtcg
     7021 gcgcttgcgg tctgccacca ctccggcgag gcgctcatca cgtattcgac cgccagcacc
     7081 gcatccttgc gccgcttctc cggcaaccgc tccctgagct tgcccatcgc ctcatcggtc
     7141 gatctggccg ccaggtgatc gttgtccggt gtgcgctcct gatcggcgtt cggcgtctcg
     7201 cggtcgcggt agcagtgctg caacgcagcc gccaccgtgc ccatgctgtt gagcttcttc
     7261 gccctcatga tcgcaaacgc catccgtccc cctccgcact gtcagattgc tgccagcccg
     7321 tcgaggtacg aatgtgcctc cggcgggcca cttctttgaa gtgtaaactc actagacttt
     7381 actccgtaaa gtacgtgacc gctacgcggt tgcagcggcc tgcggccttg ctctacgggc
     7441 ttcgccctgc gcctcccttc ggtcgttgcg aacaccttgg ggcgctgccc caaaccccgc
     7501 gttcgccacg tggcaggggt gcaggtggcc gcgccgcttc gctctgctct acctgccccc
     7561 ttgcctgagc gtgggggtca ctccatcacg tcaagggttc gcttcgccgg gtgctcaccc
     7621 gatcccttcc gcttcgctcc agggctgcgg cttccgctcc cgcccttgac ctgattccgt
     7681 tccgagtgga ggcggggtca aggggtcgtg gaataccgca aggcgcgcag cgcctgagga
     7741 tatgccgcga aagccccttg acgctgccgg aacgacccag cctctggctc tggggttggg
     7801 aacgaaggga gcgcagcgac cgcaggcccc agcccccgag cctcacggcg gcgagtgtgg
     7861 ggagtccaga ggggcaaagc ccctttgggc ctagcgcagc ggaaacaaac cctctcgcct
     7921 ctggcgaggc attaactcca agggagcgca gcgaccgtca gcagggtgcc aagcgaagcg
     7981 ccggcgggac ggcagtccct tagaggagaa ggggaaaaat actaaatgat tgatttatat
     8041 agtttttaaa ttttgtgata ggaaacgtag ggttccggac aaccggaagc agcttgtttc
     8101 cgcttacttc ctatcatagg aaacaaattg cttgacttgg gaaacggttt tgggaagtaa
     8161 ttttcctatg gtaggaatta aacgtccggt taagcggacg ccctgtttcc ggttaggagg
     8221 tcaacaatgc aaagcgtcac cgtagcgaca gagacacaga gacgaatcag gcaaagggag
     8281 ctggcagcag cagacgagaa ggaggctcag gagcgcgagg aggcgcgaaa gaacaagggg
     8341 ttcacacagg tatattcaaa gggatggcgg cgcgtcaggg agggggtctg aagtccaatg
     8401 gaacgaaaac gtacgttagt gaagtaactg tatgatatat caagacataa tggacataga
     8461 aaaacgccat caaaacggtg tctttttaat cgaaaattgg cacttaacgg acggcattgt
     8521 ctactaatcg atttttagac gataattttc ctcttaccgt acattttcgt acggttggcc
     8581 ctcaaacccc gatttgaact caatcggata acctgaatgc aataccgcaa tggcgataat
     8641 ttaaaagcga tagccgttga cgcaaaatca atccgttacg catctctatt ggttaaataa
     8701 tgcgcttaac gtacaaaatt ttcccatatc caaactgacc ccttaaagga catcaacacg
     8761 cattcggcgt tttttcttgt ccaattgtga ttttcgtgag ctccgtatca gtgaaatttc
     8821 cctttcctga ttctgtgtca aagtcaccag gtttgaaccg gtgtacatcc tgccatatag
     8881 aaaaacaggt gtggcaggtg acaggcagtt ctccatcagc caccttttcg cagttttcaa
     8941 tcagtacccg ttccttattg tattgtaagt tataaccact tgtgcaaagc gtttcatacc
     9001 cgtgagcgcc tgtcacgata tgtgtggcaa tccgttccct gacttttccg ttcactaata
     9061 actgttcacg tgtggtgatc gttcgcattc tcttcctcat cagctttcct gtagaagcct
     9121 cctgactata aggctccgga tatcttaccc gctcattttg gctggaggta gtggtggcgt
     9181 cagaggatga attactgaca ttgtatatga agctttgagt tttttctgga tttcatcagt
     9241 gtttatcttt atttgttctt gtttgttctc ttttgtgctg ttggagtcgg tttttgtctg
     9301 tatttttctt tatttttcca tttgtggccg atatcaggtc tttaccgata cgttgtttta
     9361 ggtgataatc gtttttgatc tcgtattttt ctaaaaaaat ccattttatt tatgtgaggt
     9421 tctagatgcg tatctttgta gacgacggct ctaccaacat caaactggcc tgggttgaag
     9481 acggtgcagt gaaaacgtta attagtccga atagttttaa gccggaatgg tctatgacgc
     9541 tgcagggact gactgatgcc actgtacccg cttccgcaaa ctatgagatt gatggagaaa
     9601 aatacagctt tgatcaattg agtccggatg cagtgcgcac cacagaaacg cgttatcagt
     9661 acagcgacgt taatgtggtt gctattcatc atgcgttgat gcagtcaggt attgctccac
     9721 aacctgttga tattgttgtg actttacctt taggtgagta tctggatgag aacgatcagc
     9781 caaatctggc taatatcgaa cgtaagaaaa ataatgtccg gcgaactgta acagttcagg
     9841 ggcgtgaaaa ctttaccatt cgtaaagtca gtgtcttgcc tgagtccgtg cctgccggtt
     9901 ttgacgtgct gaaagatctg aatgatctgg agtcgctgtt gattgttgat atcggtggaa
     9961 cgacccttga tattgcacat gtccgcagca aaatgtcagg tttcacaaaa acctattgtg
    10021 accccaagac tggcgtctcc atcattactg aagcagttaa gcatgcgatg gatgtgaatg
    10081 tgagtacacg aaccagttct tattttgcag atcgtctaat ccagggccgg gatgacaatg
    10141 aattcctgac ccgttatatc ccgaatgcag aacagcgtga gcggatcctg acagtattac
    10201 gtgaccggga aaaaaaactg cagcatcgtg tggcggattc agtaggacgc tttgctggtt
    10261 ttactcatgt tatggttgtt ggcggtggtg ccagccttgt ggctggggca gtaaaaaaag
    10321 cgacaggagt gggtgataac cgtttctttg tcagtgacaa tcctcagttc gatctggtgt
    10381 tgggaatgat ggcaatgaag ggatgagtat ggctgataat cgtaagtgct ctttttatat
    10441 ttatccggaa cgaaatgcag ctgatcgggt ggctgacagg tttcttgaaa aattgcctca
    10501 gaaagaacgt ggtcgggcaa tgcgggccat gatgttgtgt ggtgctgcat tgatgaagca
    10561 ggatgaacga ttgccgttcc tgatagctga attcctgaca gacagcacgt ctatgcagga
    10621 tattcaacga attatcagta gtacgctacc acaacaggaa aatggtgaag ttgttcgttt
    10681 gctggaggca tttctgcagt ctgccggtaa taatgcaaaa acgatcctgc ctgcggttga
    10741 ttctgctacg caagaaatat ctgctccagt cgatcagaat ctcctggaaa caagaaacaa
    10801 cattaagaac ttgtttccgg atgatgaata agaactgaac tctgttaaag gacgctgttg
    10861 cgtccttttt tcttttccga caaagaatgt cgctgggctc ctctttttac tgcgccggct
    10921 gacgcggcgc ggcaggaacg ctgcctgtgg tcagtgtccc gcgtccggcg ggcacgggac
    10981 gggcgctttt accgctcccg gctggcgtcg gtgacagtgt acgccagccc gtcgcccttt
    11041 tctgatgaac gcccttcaag ccgctttcgc ggcatagcct tgccgtcaaa aagacgacgg
    11101 ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc ccacaggcag
    11161 cggggaacgg gcgctacagg ggcgccctcc cggtgctctc tgctgaagca gaagtgagag
    11221 gagaattccc atgccaaact ggtgcagtaa ccgtatgtat ttttctggtg aaccggcaca
    11281 gatcgctgag attaaacgac tggccagcgg tgcagtcaca ccgctttatc gccgcgccac
    11341 aaatgaaggt attcagctgt ttctggccgg aagtgccgga cttctgcaga tcactgaaaa
    11401 tatccgctct gagcagtgcc ccggagtgac ggctgccgga cgtggtgctg tatcactgga
    11461 aaatatcgcg ttcacccgct ggctgacaca cctgcagaac ggtgtactgc tggatgagca
    11521 aaactgcctg atgctgcatg aactctggct gcagagcggt actggacagc gtcgctggga
    11581 agggttaccg gatgatgtca gggaaactat cactgtgcat ttcaccgcaa aaagaggtga
    11641 ctggtgtgac atctggggta acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa
    11701 cgtactgccg gaaaaaacca tgccgtttga cctgctgacg gttttgccga cccgcctgga
    11761 tattgaagtg aatggcttta acggcggtgt tctgaacggt gttccttctg catatcactg
    11821 gtatacagaa cggtatggcg tgaagtggcc ttgtgggtat gacctgaata tcagcagtca
    11881 gggggaaaac ttcattcagg tggatttcga cacgccgtgg tgtcagccgg aaagcgacgt
    11941 tgttgcagaa ttaagccgcc gtttcggctg cacgctggag cactggtacg ccgaacaggg
    12001 ctgcaatttc tgtggctggc agttgtatga gcgcggagag cttgttgatg tgctgtgggg
    12061 ggaactggaa tggtcttccc cgacagatga cgatgagctg ccggaagtca ccggacctgc
    12121 gtggatagtc gacaatgtga cgcattatgg cggatgaagt atgacggaga cgggcgggca
    12181 accgcccgtt tcttttccga caaaggatgt cgccgtgctc ctctttttac tgcgccggct
    12241 gacgcggcgc ggcataaacg ctgcctgtgg tcagtgtccc gcgtccggcg ggcacgggac
    12301 gggcgctttt accgctcccg gctggcgtcg gtgacagtgt acgccagcct gtcgcccttt
    12361 tctgatgaac gcccttcaag ccgctttcgc ggcatagcct tgccgtcaga aagacgacgg
    12421 ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc ccacaggcag
    12481 cggggaacgg gcaccacagg ggtgccctcc cggtgtcctc tgtaagagaa ggagcgttgt
    12541 tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtattc ctgataacgc
    12601 cattgatttc atactgactg acccgccgta cctcgtcggt tttcgtgacc gtttcgggcg
    12661 caccatcgct ggcgataaaa ccgatgaatg gctgcaaccg gcctgtaatg aaatgtaccg
    12721 cgtactcaaa aaagacgcgc tgatggtgag cttctacggc tggaaccgcg tcgatcgctt
    12781 tatggccgcc tggaaaaatg cgggattcgg cgttgttggt cacctggtct tcaccaaaaa
    12841 ctacacatcg aaggccgcat atgtgggcta tcgccacgaa tgcgcctaca tcctggcaaa
    12901 aggacgtccg gcgctgcctc agaagccatt gcccgacgtg ctgggctgga aatattcagg
    12961 caatcgtcat cacccgactg aaaagcccgt taccagcctg caaccgctca ttgagagctt
    13021 cacacatccg aacgcgattg tgctggaccc gtttgcaggc agcggatcaa cctgcgtggc
    13081 cgctcttcag tccggacgcc ggtatatagg tatcgagctg cttgaacagt atcaccgtgc
    13141 cggacagcaa cgactgaccg ccgtgcaacg ggccatgcag cagggggccg cgaatgataa
    13201 ctggtttgaa ccggaggctg cgtaaatgaa ctatgccgga cacgaaaaac ttcgcgccga
    13261 cgtggcggag gtggccaaca ccatgtgtga ccttcgggcc aggctgaacg atatggagca
    13321 tcgctgtcgc tttgattccg atgtgcttgt tgaacgcctg acacgccaga cgctgtatcg
    13381 cgccaatcgc ctgtttatgg aggcgtatac cgaaattctg gaactggatt cctgcttcaa
    13441 agattgagaa ggagacgaga atgtacggaa catgcgaaac gctctgccgt gagctggcag
    13501 cgcagtatac ggagaacacg ccgctgatgc tggttgtctg gtccccggaa gagattcagg
    13561 cactggctga cggtatggaa atttctctga ccggtcatga aatcaggacc gtcctggcac
    13621 gcctggagga catcccggaa gaacagcgga ttgagtccgg tatttcttcc gctgcggtga
    13681 tggagatcat caataacgtg agcgaaaacc gccaggtgac cgtccctgct gaactgctgg
    13741 cgtccctgat tcagaccgct gagcaggcat tgtggaaacg tgaatgggcc gcccgggatc
    13801 atggcctcgc cgtcccggaa tgcgtcaccc gccgtcagga ggtggttaat caggcccgca
    13861 ccctgctgaa aaacaacaca cacgaaaacg actgatgtta tcgccgcctc cgggcggcga
    13921 tacagggagt tcatgaatta tgaatgaaac gctgaatgca ctgatttatc gccacgccag
    13981 caacctgttg ctggcgcagg gctggccgga agaaacggat gttgaccagc gtaacccgaa
    14041 gtatccgggc tgggtcagca tttacgtgcg gctggatgcg ccccggctgg cgacgttact
    14101 tatcaaccgt cacggcggcg tactgccgcc gctcctggcc tccgccattc acaaactgac
    14161 cggaaccgga gcggaacttg ttctgtccgg cagtcagtgg cagtcgctgc cggtacttcc
    14221 ggcagacgga acgcaggtgt ctttcccgta tgccggtgag tggctgaccg aagacgaaat
    14281 cagggccgtt cttgatgcgg tacgcgatgc agtatgcagt gtcagttacc aggtggcaga
    14341 agatgcgcgg cgtatccgtg cggcgctgac caccaccggt cagacgttgc tgacccgcca
    14401 gacgcgccgc tttcgcctgg tggtgaagga aagcgatcac ccctgctggc tcgatgaata
    14461 cgacgaaaac ctgcccgtgg tgctcgatgc catcctgaac cggggcgctc gtttttcgtc
    14521 ggtggaaatg tatctggtca gtgagtgtgt ggagcatatc ctgtccagtg ggctggcctg
    14581 cgatgtgctg cgtataccgg atgaaccgcc ccgccgctgg tttgaccgtg gtgttctgcg
    14641 ggaggtggtc ctggaagccc ggagtgaaat ccgcagcatg gcggatgcac tggcaaaaat
    14701 caggggctga tgatggcggg gaaaatcccc gcctttttcc tgccggtccc cggaaagagc
    14761 aaaaggatgt cgccgcaggc tccgctttac ccggccatgc ggggcatggc cttgtgggtt
    14821 ttcagttatg tggcctcagc gtcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc
    14881 ttctgcgggc gggatgcaag gtaagatgaa ccgctgcggt cacccggtcc ctccttcccg
    14941 tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc ctccaactcc
    15001 cgccagccgt gtgcgtcagg ctgcggcttc ccttgcgccc ttgcaaccct gcaggcccgg
    15061 caaagatgga ggcacggcac cgcccggtgt cgcggaatat gtgaacaacg gaggaaaaca
    15121 tcatgcaata tgcgaaacct gtcactctga atgttgaaga gtgcgaccgt ctctcctttc
    15181 tgccttacct gtttggcctg gattttctgt atgccgaggc atctgtgtac gcgctggcga
    15241 aaaaaatgat gccggaatat gaaggcggat tctggcactt catccgcctg ccggacggtg
    15301 gcggttacat gatgccggat ggcgaccgtt ttcatctggt caacggtgaa aactggtttg
    15361 accgtaccgt gagtgctgat gccgcaggca tcattttgac ttcccttgtg attaaccgcc
    15421 agttgtggct gtaccacgac agcggggatg caggactgac ccatctttac cggatgcgcg
    15481 atgcgcagtt gtggcgtcac atcgaatttc accccgaatg caacgcgatt tacgcggcgc
    15541 tggactgatt aacggacggg gcggcaatgc cgcccctgta aaggagacga gaacatgtac
    15601 tgtactgtta aagaaattat ccgcgaagtg ctggatacag acgtgccgga cagtgaatgc
    15661 gtttttgccg tggtgctgac tcgtggggat gtgcgtcaca tagcccagga ctggagtctg
    15721 acagacgatg agctggaaac cgtcatgcag cggctggacg atgcctttga gcatggtgcg
    15781 gatgtcagcg ttgttcacga cgttgttcgt gaactgatgg aggaaaagca cgccagccgt
    15841 caggtgacag tcccggcggt gatgctggaa aaagtgatgg cgctggcagg cagtgaaatg
    15901 aagcgcctgt atgccgtcgg gagtgagaac gggggcgacg gtgatgcgtt cgtcagggaa
    15961 gaacgcgaag caatggacgt tgtgttacag gcgctggacg gggagcacat gtcatgaata
    16021 tcagcacaga aacccgcgaa attctgcgta attacagggc cgtgattaat gcgcggcgtc
    16081 gtgaaatggg gcagaaaccg ctcaccaccg cgcagattat tgatgaaatc tgcgattttg
    16141 tggcgaatca gcaggcggtt ttcctgggcg gtcactacat cttacagggc agcagaaaca
    16201 ggtgatacag gggcaggcgg tggaggccgc ctgtcagccc gcgcgaaaac ccctggcagg
    16261 ctgacgcctg ccggaaggga agcgggccac gctggcgcgt cgcccgttac ccttcccgtc
    16321 tgtgatgtgt ctgactcagg agtgtttctt tcttatgtta tggaactgtt cctgatgttg
    16381 ttgtcgttgt tcctttatct gctgctctga ttctgactac tgcagtgttg ttctgtctgc
    16441 cggacgcacc ttcggctccc ggctgcctgg cggcatccgg tcgtcaggcc cgactccgac
    16501 aggcagctgc tggcgcaccc gccagtctcc gccgaacccg ctggcgcggt ttcgggctgt
    16561 cgctacggtc cgatgcgtga aaacccccgg cttgcagcga ccgttccggt cgcgccgcct
    16621 ctgtcgcgcc gtggcctgcg ctcccggcat cccggcaaaa ggcctccggg atgtaaggcg
    16681 caacgccgtt tcgcgcacgc gctcttcgtc gttgcagcgg gcccctgctc attgcctgcc
    16741 gggtgccaca cacccgtatg agtccctgtc gccgggggct gatacagcca caacctgccg
    16801 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
    16861 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
    16921 aaggttctca aacgggtgaa gttcatacca cagggaatac agcagcaggt tcccgcgaaa
    16981 cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc tttaaaaatc aggatttatt
    17041 tcagactccg gcaatacgca tttatgtgtt ctgtcagcat cctgctggca ggtaatgaga
    17101 acgcaggaac agaaggagga atacaccgga gaacgtcaga cgcagatatg aaaaaagcag
    17161 gccggggagg acctgcttaa tccacaccgc aaaaactgtt cgagcagttt ttgcggctcc
    17221 agtcaatcgt cataagaggc taaattatga caataagtat tgtttcacct tccgcagcgg
    17281 ctgtaaagcc ccgccgtcat ccccgtttca gacgcgaaga catccccgcg ccggaaattg
    17341 accccgtgct gaaagccttc gggcgacaca tagcccggag ttttcaccgg gggcgtggtg
    17401 tacacatccc cgccatgaaa aacacggcct tcgggcaggt gctccgcaca ctcgaactga
    17461 aacgcgcttt taactgatga ggtgagccgc cccgcgaggg gcggcaacag acggaaggag
    17521 aacattttat gacattagca gcaatcacca tgaccgcccc ggaagccgcc agccctgtgc
    17581 agatgtaccg cgcgacctac tcaccggatg acaacaaact gcgcctgtat gccgcgtcac
    17641 gtcttgaccc ggagacgtat aaaaaagtgc atgatgccgg ttttcgctgg gcacccagac
    17701 aggcgctgtt tgtcgcgcca gcctggacac cgggccggga agacgtgctc ctctcacttg
    17761 ccggagagat tgaggatgaa gacagcacgc tcgctgaacg tcaggaagca cgggcggagc
    17821 ggtttaccgg atacagcgga aagcgggcca gtgaatccgc acaggcactt gatgaagtgg
    17881 aaagactggc cgcgatgatc ccgcccggtc agcccattct tgtggggcat cacagcgaac
    17941 gccgcgcccg tcgtgatgca cagcgtattg aaaacggtat gaaacgtgct gtgatgctct
    18001 ttgaacgtgc ggaatactgg gaagagcggg cgcggtcggc actgcttcac gcgaagtata
    18061 aagaacgtcc ggacgttcgc tggcgtcgta tcaaaaaaat cgaagctgat ttgcgcaagg
    18121 cggaaaagac catcgcacag tcgcagaaat atctgacgat gtggcgggct gaatcgctgg
    18181 atctgaatat ggcaaaactc atcagcagtc atgaccatat cagcgcctgt ttcccgctgg
    18241 acaggtatcc gcgcccggca gaaaaaagcc agtatgaagg aagtcgatcg ttatggtcgg
    18301 ccctggatga tgacatcatc accacggagc aggcccgcga aattgcgatc cgctgtcatg
    18361 aacggcagat tcagcatcag caacgctggg ttaaccacta tcagaaccgc ctgatctatg
    18421 agcgtgccat gctggacgaa agcggcggcg tggttacccg gacacaggat tttgagccgg
    18481 gcggacaggt tttcagccgg ggcgagtggc tgaccatcat ccgcgtgaac aaaagcaacg
    18541 gggcggtgag ttcagtcaca acgccgaatt acagttttct cgggtacagc ggcacgatga
    18601 aagtgacgcc cgatcgcatc acggactaca aagcaccatc ggcagaagag gctgccgtcg
    18661 ccagtcaggc cgcgaagcgt ccgccggtag tcaactatcc gggggacggt ttccgggaaa
    18721 tgacaaaggc gcagtgggcc gccctgcccc gggactgtaa ggccgtgcgc agtgtggcag
    18781 agacagaaga ccacggggca taccgctacc gccgcacaat ggacaataat ttccgcctgg
    18841 tgaatgtgta tattaccgac atgaaaatca cggaaatccc acagaaataa ggtatatccc
    18901 cccgggaaat ccccggggga tgacggagat aatgacatgc acagtcagtt aagagaacgt
    18961 atccggctga tgcgcgcaag gctggataac gccgcgccgg ttgctgaaat ccgggctgaa
    19021 tctcagcttt ttgtgactcc tgccccggtc tgtgatcgcc tggtgacgct ggcggaaatc
    19081 agcaaccgtg atcacattct ggaaccctct gccggcaccg gggccatact gcgggcgatt
    19141 cgggataccg cgccggaggc catgtgtgat gcggtggaga ttaacagcgg gctggtccgg
    19201 tatctgcggg aaaattttaa cggtgtcagg gtacagtgtg gcgacttcat ggaatggcag
    19261 ccggtgcagt attacagccg gatcattatg aatccgccgt tcatccacgg gcaggatatc
    19321 cggcatatcc tgcgggcctt ttccctgttg cgtccggggg gcgtgctggt tgccgtctgt
    19381 ctgaacgggc cgcgccagca ggagaagctg ttaccgtttt ctgacatccg cgaggagctg
    19441 ccgcgcggca cgtttgctta taccgatgtc ccgacgatga ttattcgttt gcgtgcctga
    19501 tgacacaggg ctggcgggag gataaatgcc cgtctgtccg cgccgggaaa agacagcacg
    19561 ccggtggcgc tgaaaggggc cgcgcttacg cgccgcccct ttcattacgc ggatgttctg
    19621 ctgtgtgctt ctgtcttttc atgcctgtca tttgtctgtt actgatgatg ttgttgttcc
    19681 tgctactgct actgctactg ctactgctac tgctactgct actgctactg ctcttctttt
    19741 tcctgccgct ctgattctga ctactgcagt gttgtcctgt ctgccgggcg caccttcggc
    19801 tcccggctgc ctggcggcat ccggtcgtca ggcccgactc cgacaggcag ctgctggcgc
    19861 acccgccag
//
LOCUS       DAZFDP010000082        19864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFDP010000082 DAZFDP010000000
VERSION     DAZFDP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHJ1G_003019"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHJ1G_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4764834.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHJ1G_003020"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHJ1G_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4764835.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHJ1G_003021"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHJ1G_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4764836.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHJ1G_003022"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHJ1G_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4764837.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHJ1G_003023"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHJ1G_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4764838.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHJ1G_003024"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHJ1G_003024"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4764839.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHJ1G_003025"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHJ1G_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4764840.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHJ1G_003026"
     CDS             complement(7218..7283)
                     /locus_tag="ACHJ1G_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764841.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHJ1G_003027"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHJ1G_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4764842.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHJ1G_003028"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHJ1G_003028"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4764843.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHJ1G_003029"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHJ1G_003029"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4764844.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHJ1G_003030"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHJ1G_003030"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4764845.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHJ1G_003031"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHJ1G_003031"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4764846.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHJ1G_003032"
                     /pseudo
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHJ1G_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHJ1G_003033"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHJ1G_003033"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4764847.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHJ1G_003034"
     CDS             15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHJ1G_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4764848.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15939..18221
                     /gene="fiu"
                     /locus_tag="ACHJ1G_003035"
     CDS             15939..18221
                     /gene="fiu"
                     /locus_tag="ACHJ1G_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4764849.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18263..18940
                     /gene="ybiX"
                     /locus_tag="ACHJ1G_003036"
     CDS             18263..18940
                     /gene="ybiX"
                     /locus_tag="ACHJ1G_003036"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4764850.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19014..19280
                     /gene="ybiI"
                     /locus_tag="ACHJ1G_003037"
     CDS             19014..19280
                     /gene="ybiI"
                     /locus_tag="ACHJ1G_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4764851.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYNRRGSKDSQLR"
     gene            19545..19805
                     /gene="ybiJ"
                     /locus_tag="ACHJ1G_003038"
     CDS             19545..19805
                     /gene="ybiJ"
                     /locus_tag="ACHJ1G_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4764852.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         4904 a         5203 c         4886 g         4871 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccatcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgctctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcataatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactagc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggcggag ctgcgtccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    13681 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    14341 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    15721 gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg
    15781 cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg
    15841 cacgaacact atcacgagat taacagtggc atcgcatccg cagagaggct ttctcgtggc
    15901 agtgaaaatt tcaacatata aaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc
    15961 ctgccagaca atttcattcg ctcacgttct ttgccggtct ttgtattggc atcacgcctg
    16021 tggctcaggc actcgccgcc gaagggcaaa ctaacgcgga tgacacgctg gttgtcgaag
    16081 catcaacgcc ttcgctttat gcgccacaac aatctgccga tccgaaattc tcgcgtccgg
    16141 tagcggatac tacccgcacg atgacggtga tttctgaaca agtgattaaa gatcagggcg
    16201 caaccaacct taccgacgcg ctcaaaaacg tccccggcgt gggtgcgttt tttgcgggtg
    16261 agaacggtaa ctccaccact ggcgacgcca tttatatgcg cggtgctgat acctctaaca
    16321 gtatttatat tgatggcatt cgcgatatcg gcagcgtctc gcgcgacacc ttcaatactg
    16381 agcaggtcga agtgattaaa gggccttccg gcaccgacta cgggcgcagc gcgccgacgg
    16441 gctcgatcaa tatgatcagc aagcagccgc gcaatgattc cggcattgac gcctccgcca
    16501 gtattggcag cgcctggttc cgccgcggca cgctggacgt caatcaggtc attggtgata
    16561 ccactgcggt gcgcctgaat gtgatgggcg aaaaaacgca cgatgccgga cgcgacaaag
    16621 tcaaaaatga gcgttacggc gtcgcccctt ctatcgcttt tggccttggt acagcgaatc
    16681 gtttgtatct taattatctg catgtcaccc agcacaacac gccagacggc ggcattccga
    16741 ccatcggttt gccgggttat tctgccccat ctgcgggaac agcggctctg aatcattccg
    16801 gaaaagttga tactcataac ttttacggca cggattccga ttacgacgat tcgaccaccg
    16861 acaccgccac catgcgtttt gagcacgaca tcaacgataa caccaccatt cgcaatacta
    16921 cccgttggtc gcgcgtaaag caggattacc tgatgacggc gattatgggc ggggcgtcga
    16981 atattactca gcccaccagc gatgtgaata gctggacctg gtcacgcacg gcgaatacca
    17041 aagatgtgag taataaaatt ctcaccaacc agaccaacct gacctcgacg ttctataccg
    17101 gttctatcgg tcatgatgtc agtaccggcg tggaatttac ccgtgaaacg cagactaact
    17161 acggcgttaa tccggtgacg ttacccgcgg taaatattta tcatcctgac agcagcattc
    17221 atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg tcagacggat accttcgcaa
    17281 tttacgcctt cgatacgctg caaatcaccc gtgattttga gctgaacggc gggatccgtc
    17341 tggataatta tcatactgaa tatgacagtg ccaccgcctg cggcggcagc ggacgcggtg
    17401 ccatcacctg cccaactggt gtggcaaaag gttctccggt caccaccgtc gacaccgcca
    17461 agtcgggcaa tctggtgaac tggaaagccg gggcgctgta tcacctgacg gaaaacggta
    17521 atgtctatat taactatgcc gtttcccagc agcctccggg cggcaacaac ttcgcccttg
    17581 cgcagtctgg cagcggtaac agtgccaacc gcaccgattt taaaccacaa aaagccaaca
    17641 ccagcgagat tggcaccaaa tggcaggttc tggataaacg cctgttgctc accgccgcgc
    17701 tattccgtac tgatatcgaa aatgaagttg agcaaaatga tgacggaact tactcgcaat
    17761 acggtaagaa acgtgtcgaa ggctatgaga tatccgtggc cgggaatatc acgcccgcgt
    17821 ggcaggtgat tggcggctat acccagcaaa aagcaaccat caaaaacggc aaagatgttg
    17881 cccaggatgg ttcctcatcg ctgccgtata ccccggagca cgccttcacc ttatggagcc
    17941 agtatcaggc aacagatgat atctctgttg gcgcgggcgc acgctatatc ggcagtatgc
    18001 ataaaggttc agacggcgcg gtgggaacgc cagcgtttac cgaaggttac tgggtcgccg
    18061 atgccaaact ggggtatcgg gttaatcgca atctcgactt ccagctaaac gtttacaacc
    18121 tgtttgatac cgattacgtc gcctcaatca acaagagcgg ctaccgttat cacccgggcg
    18181 agccaagaac cttcttgctc acagccaata tgcatttctg attcagatgt ggggcgcagg
    18241 ccccactttt tggagaactt gtatgatgta ccacattccc ggcgtgttat cgccacagga
    18301 cgtcgcccgg tttcgcgaac aactggaaca agccgaatgg gtggatggac gcgtcaccac
    18361 cggcgcacaa ggtgcgcaag ttaagaacaa tcaacaggtc gacacccgca gcacgttata
    18421 cgccgccctg caaaatgagg tgctgaacgc ggttaaccaa catgctttat tctttgccgc
    18481 ggccttgccg cgtacccttt ccacgccgct gtttaatcgc tatcagaaca atgaaaccta
    18541 tggtttccat gtggatggcg cagtacgcag ccatccacaa aacggctgga tgcgtactga
    18601 cctttctgcc acgctgtttt taagcgatcc acaaagctac gacggcggcg aactggtcgt
    18661 taatgacacc ttcggacaac atcgggtaaa actcccggca ggcgatctcg tgttgtatcc
    18721 ctccagcagc ctgcattgcg tgacacccgt aacccgcggc gtacgagtgg catcatttat
    18781 gtggatccag tcgatgatcc gtgatgataa aaagcgcgcc atgctgtttg aactggacaa
    18841 caatattcag tcgctgaaaa gccgctacgg cgaaagtgaa gagatcctgt cgctgcttaa
    18901 tctttatcat aatctgctgc gggaatggtc ggagatctga tgcttgaact gcccaggttc
    18961 gtccctggat tagctacact taactgtaca agcattgata tggggaggac gatatggcat
    19021 ccggttgggc taacgatgac gccgtcaacg aacagatcaa cagtacaatt gaagatgcga
    19081 ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct ggatgaatgt gaagagtgcg
    19141 gtgcccccat cccgcaagcc cgtcgggaag ccattcctgg cgtgcgctta tgcattcatt
    19201 gtcagcagga gaaaggttta caaaaaccag cgtatacagg atataatcgc agaggttcga
    19261 aagacagcca gttacgttaa ctacaactgg cgagatgcat agcgagtata atttgtattt
    19321 tgcgtggcat tccgtgcctt taacgctgct gtgactgcca cgcggcataa aacaagtcca
    19381 taagtacaac aaataaatgg tttatcagta tgttagatat caatcaattt atttgaacaa
    19441 ggcggtcaat tctcttcgat tttatctctc gtaaaaaaac gtgatactca tcacatcgac
    19501 gaaacaacgt cacttataca aaaatcacct gcgagagatt aattatgaaa actatcaata
    19561 ctgttgttgc tgctatggct ctttcaactc tgtcatttgg cgtgtttgcc gcggaaccgg
    19621 taacggcatc ccaggcacag aacatgaata aaatcggcgt ggtttctgcc gatggcgcat
    19681 ccaccctcga tgccctggaa gcgaaactgg ctgagaaagc cgcggcagcc ggtgccagtg
    19741 gatacagcat cacttccgcc accaacaaca ataaattaag cggtactgcg gtaatttata
    19801 agtaattcgt aggacggata aggcgtttac gccgcatccg gcaacagatg cctgatgcga
    19861 cgct
//
LOCUS       DAZFDP010000083        19847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFDP010000083 DAZFDP010000000
VERSION     DAZFDP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHJ1G_003039"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHJ1G_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4764853.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2773)
                     /gene="hemX"
                     /locus_tag="ACHJ1G_003040"
     CDS             complement(1574..2773)
                     /gene="hemX"
                     /locus_tag="ACHJ1G_003040"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4764854.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2795..3535)
                     /gene="hemD"
                     /locus_tag="ACHJ1G_003041"
     CDS             complement(2795..3535)
                     /gene="hemD"
                     /locus_tag="ACHJ1G_003041"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4764855.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3532..4473)
                     /gene="hemC"
                     /locus_tag="ACHJ1G_003042"
     CDS             complement(3532..4473)
                     /gene="hemC"
                     /locus_tag="ACHJ1G_003042"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4764856.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4860..7406
                     /gene="cyaA"
                     /locus_tag="ACHJ1G_003043"
     CDS             4860..7406
                     /gene="cyaA"
                     /locus_tag="ACHJ1G_003043"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4764857.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7446..7766)
                     /gene="cyaY"
                     /locus_tag="ACHJ1G_003044"
     CDS             complement(7446..7766)
                     /gene="cyaY"
                     /locus_tag="ACHJ1G_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4764858.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7814..9139)
                     /locus_tag="ACHJ1G_003045"
     CDS             complement(7814..9139)
                     /locus_tag="ACHJ1G_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4764859.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9136..10644)
                     /locus_tag="ACHJ1G_003046"
     CDS             complement(9136..10644)
                     /locus_tag="ACHJ1G_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4764860.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(10982..11536)
                     /locus_tag="ACHJ1G_003047"
     CDS             complement(10982..11536)
                     /locus_tag="ACHJ1G_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO4764861.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11886..12089
                     /gene="yifL"
                     /locus_tag="ACHJ1G_003048"
     CDS             11886..12089
                     /gene="yifL"
                     /locus_tag="ACHJ1G_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4764862.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12126..12950
                     /gene="dapF"
                     /locus_tag="ACHJ1G_003049"
     CDS             12126..12950
                     /gene="dapF"
                     /locus_tag="ACHJ1G_003049"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4764863.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12947..13654
                     /gene="yigA"
                     /locus_tag="ACHJ1G_003050"
     CDS             12947..13654
                     /gene="yigA"
                     /locus_tag="ACHJ1G_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO4764864.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13651..14547
                     /gene="xerC"
                     /locus_tag="ACHJ1G_003051"
     CDS             13651..14547
                     /gene="xerC"
                     /locus_tag="ACHJ1G_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4764865.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14547..15263
                     /gene="yigB"
                     /locus_tag="ACHJ1G_003052"
     CDS             14547..15263
                     /gene="yigB"
                     /locus_tag="ACHJ1G_003052"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4764866.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15347..17509
                     /gene="uvrD"
                     /locus_tag="ACHJ1G_003053"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15347..17509
                     /gene="uvrD"
                     /locus_tag="ACHJ1G_003053"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4764867.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17656..18420)
                     /gene="yigE"
                     /locus_tag="ACHJ1G_003054"
     CDS             complement(17656..18420)
                     /gene="yigE"
                     /locus_tag="ACHJ1G_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO4764868.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18582..18638
                     /gene="ysgD"
                     /locus_tag="ACHJ1G_003055"
     CDS             18582..18638
                     /gene="ysgD"
                     /locus_tag="ACHJ1G_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4764869.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18790..19740
                     /gene="corA"
                     /locus_tag="ACHJ1G_003056"
     CDS             18790..19740
                     /gene="corA"
                     /locus_tag="ACHJ1G_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4764870.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4606 a         5158 c         5180 g         4903 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tctgctggcg cagcactttt tccagctgtt ccggattgtt ggttttcagt
      661 cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctctgct
     1141 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcagtct ggatcagcac gtaaccctga
     1501 tgaccggcaa tcatcgggcc aaccactatc cccgcaatca gcaaaacaaa gagtaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcacct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca
     1681 gcaggttacg cacgcgggtc tgcatcagtt tttccagcat cgcctggctt tgcagggttt
     1741 ccggaagatc catcgagata ttttgctggc ttaactggtc cacctcgtcg aggaacgctt
     1801 tggtggtggc atcatcagta tcgtagtaag ctcgtaccca ggtggagacg ttctccagcg
     1861 cctggcgata agtctcttcc tggtgacgcg gtacagcttg tgctgcgacc agcaggcgag
     1921 agcgaatatt ttcgcgcaga tagatatcct gatttggcgc taacagcggt acggcggtgt
     1981 catcacgacg gcgaatcgta atgaagttgt ccataaagtt ctgccagctt ttttgcagat
     2041 tgatacgcca ttcgctgatg gaactggaaa gctcttcacc gtcggaatcc atcggcgaac
     2101 catcgctgtc attatcggcc agacgcaggt tatctacctg atttgaaagc tgattaagct
     2161 taaggatgat gccgtcataa tccacctgcg aaactgcaga aaggctggcg atatcatcgg
     2221 taattgcccg acgaacggta atcagactcg ggtcattcat atccgccagg ctggcatctg
     2281 cacttttcag caacgctgca gcggtcgtga cgtcctgatc gctccacagc ttccgtccgg
     2341 cgagtttcac cagaaaatcg gcctgagcca gcagccaggt tttagcatcg ctgccggaaa
     2401 tggtggcgac cttttgttgg acttcatcca actgttttgc cagcgtttct tgctgacgat
     2461 tcgcctgctt aagctgtgca gcttgttgct taataatgcc ttccagctcg gctttttggc
     2521 tctcctgggc tttttgcaat gccgtcagtt ggttagccag ggcatcgctg gtggcggtct
     2581 gattgacggc ctgttgttta ccccagccat acaaaccgac acccgccgcc agagcaatag
     2641 cgatagccac cgcgctgaga atcaatgcag tattgttctt actctttttt tctgttgcga
     2701 caggttgtga cgtggtgtcc acggcctccc tggtctcttc aaccacggcg gaggtttttt
     2761 cttgttccgt cattatggct tcctgttatg agagttattg taatgcccgt aaaagcgcat
     2821 cgttgtcagc gttatcggcg accttaatgt cttgccagcc cagttcccgg gcgagtttcg
     2881 ccaaacgctc actgacgacc aatagtcgac agtgtagtaa ccagtgctca cgataccatt
     2941 gtgggatcag cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg accatcgtca
     3001 cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt
     3061 gataacattc acaaaaagtg acctcagcac cgcgcgctgt cagggtatcc ccaattagct
     3121 cacgaccgcc attgccacgt aatatcagcg cacgtttgcc cgcaatattt tgtaattcag
     3181 gtaattgtag caagacttcg ctgatttccc gatcctgcgg gtagagaatc ttctgtccgc
     3241 ttacggtatg tagcgccagt gcggtggtgc gtccaatggc gaaataatca ggtagtcggg
     3301 gccatttacg atcttgctga tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga
     3361 gggcaaacaa cagatcgctc tcccccagcg ccgccagttg atcagcaagt tgcggtaatt
     3421 gtcgacccgg agaaaactca atcagcggaa aatgccaggc cacctgcccc agtgtgcgca
     3481 gacggctcac taactcttct ccagcgggag acgggcgggt gaccaggata ctcatgccgg
     3541 ggcgtctccg ttatagactt cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc
     3601 tgccagcgaa atccccattt gttcggcatt ttgcggcgca ccgcggcgtt caccgcgaat
     3661 aatctgcgaa ccgtccggcg cgccgaccag cgcacgcagc cagatttcac catcaataag
     3721 ctcggcgtag ctaccaattg gcacctgaca tccaccttcg agacgggtat tcatggcgcg
     3781 ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt
     3841 gcgcgtatca tcaaggcggc attcaatacc gactgcacct tgtcctaccg ccggaagaga
     3901 aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc
     3961 tacggcaaga atgatggcat cgtattcgcc gttatctagt ttgctcaggc gagtgccgac
     4021 gttgccgcgc agggagcgga taatcagatc cgggcggcgt tcagccagtt ggcactggcg
     4081 acgtaaactg gacgtcccga cgatactgcc tgccggtaac gcatccagac tgtcataatt
     4141 attggacaca aaggcatcgc gaggatcttc acgctcacaa atagtgacca accccagacc
     4201 ttgcgggaat tcaaccggca catctttcat tgaatgtacg gcgatatcgg cgcgattctc
     4261 caggagcgcg acttccagct cttttacaaa taagcctttt ccgcctactt tcgccagcgg
     4321 cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc agttcaacca ccaggcccgg
     4381 atggctcgcc atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg
     4441 gcgtgtggca attcttaaaa cattgtctaa catgcttgtt accgtcatta tcatccgtgg
     4501 tccatcctaa catccttgcc agagtgatgt cagtgttgtg gtgaaacgta gacgcctgcg
     4561 caaaccgtaa aatgaggtct ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa
     4621 ggaatttaca gagaataaac ggtgctacac ttgtatgtag cgcatctttc tttacggtca
     4681 atcagcaagg tgttaaattg atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa
     4741 accaggcgcg aaaagtggta acggttacct ttgacatacg aaatatcccg aatgccgcgt
     4801 gttaccgttg atgttggcgg aatcacagtc atgacgggta gcaaatcagg cgatacgtct
     4861 tgtacctcta tattgagact ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg
     4921 atcgcgcgct tgctgctatg gggcctgcat tccaacaggt ctacagtcta ctgccgacat
     4981 tgttgcacta tcaccatccg ctaatgccgg gttaccttga tggtaacgtt cccaaaggca
     5041 tttgccttta cacgcctgat gaaactcaac gccactacct gaacgagctt gaactgtatc
     5101 gtggaatgtc agtacaggac ccaccgaaag gtgagcttcc aattactggt gtatacacca
     5161 tgggcagcac ctcgtccgta gggcaaagtt gttcctctga cctggatatc tgggtctgtc
     5221 atcaatcctg gctcgatagc gaagagcgcc agttactaca acgtaaatgt agtctgctgg
     5281 aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt cctgattgat gaaaaccgct
     5341 tccgtcataa tgaaagcggc agcctggggg gcgaagattg tggctccacc cagcatatac
     5401 tgctgcttga cgaattttat cgtaccgccg tgcgtctcgc cggtaagcgt attctgtgga
     5461 atatggtgcc gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg ctttacgcgc
     5521 agggcgtgct gacgccaaat gaatggctgg atctcggtgg cttaagctcg ctttctgctg
     5581 aagagtactt tggtgccagc ctttggcagc tctacaagag tatcgattcc ccatacaaag
     5641 cggtactgaa aacactgctg ctggaagcct attcctggga atacccgaac ccacgtctgc
     5701 tggcgaaaga tatcaaacag cgtttgcacg acggcgagat tgtatcgttt ggtctcgatc
     5761 catactgcat gatgctggag cgtgttactg aatacctgac ggcgattgaa gattttaccc
     5821 gtctggattt agtacgtcgc tgcttctatt taaaagtgtg cgaaaagctc agccgtgaac
     5881 gcgcctgcgt aggctggcgt cgcgcagtgt tgagccagtt agtgagcgag tggggttggg
     5941 acgaagctcg tctggcaatg ctcgataacc gcgctaactg gaagattgat caggtgcgtg
     6001 aggcgcacaa cgagttgctc gacgcgatga tgcagagcta ccgtaatctg atccgctttg
     6061 cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga tatcggcgtg ctgacgcgta
     6121 agctgtatgc cgcgtttgaa gcattaccag gtaaagtgac gctggtaaac ccgcagattt
     6181 cacccgatct ctcggaaccg aatctgacct ttatttatgt gccgccgggc cgtgctaacc
     6241 gttcaggttg gtatctgtat aaccgcgcgc caaatattga gtcgatcatc agccatcagc
     6301 cgctggaata taaccgttac ctgaacaaac tggtggcgtg ggcatggttt aacggcctgc
     6361 tgacctcgcg cacccgtttg tatattaaag gtaacggcat tgtcgatttg cctaagttgc
     6421 aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg cttacctgca ccgacaccga
     6481 aggcgctcta cagcccgtgt gagatccgcc atctggcgat tatcgttaac ctggaatatg
     6541 acccgacagc ggcgttccgc aatcaggtgg tgcattttga tttccgtaag ctggatgtct
     6601 tcagctttgg cgagaatcaa aattgcctgg taggtagcgt tgacctgctg taccgcaact
     6661 cgtggaacga agtgcgtacg ctgcacttca acggcgagca atcgatgatc gaagccctga
     6721 aaactattct cggcaaaatg catcaggacg ccgcaccgcc agatagcgtg gaagtcttct
     6781 gttatagcca gcatctgcgc gggttaatcc gtactcgcgt gcagcaactg gtttctgagt
     6841 gtattgaact gcgcctttcc agcacccgcc aggaaaccgg gcgtttcaag gcgctgcgcg
     6901 tttctggtca aacctggggg ttgttcttcg aacgcctgaa tgtatcggta caaaaactgg
     6961 aaaacgccat cgagttttat ggcgctattt cgcataacaa actgcacggc ttgtcagtgc
     7021 aggttgaaac caatcacgtc aaattaccgg cggtggtgga cggctttgcc agcgaaggga
     7081 tcatccagtt cttcttcgaa gaaacgcaag acgagaatgg ctttaatatc tacattctcg
     7141 acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg cagcaaagag gagctggtac
     7201 gtgacgtcag tcgcttctac tcgtcatcgc acgatcgctt cacctacggc tcaagcttta
     7261 tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgt gaacaggtga
     7321 ttccgttccg cacaaaatct atcggtaaca tgccgcccgc caatcaggac aacgatacgc
     7381 cgctattaca gcagtatttt tcgtgatgag cgtgccggaa agcgaggctt atccggcatg
     7441 caatcttagc ggaaactgac tgtttcaccc gcttgctgtg tcgccgcctg ttccagcaaa
     7501 tcccagaagg tttcgccgct gcgatcgcaa atccattcat cgccttttag gtcaaaatgg
     7561 tagccgccct gtttggttgc cagccacacc tggtgcagcg gctcctggcg gttaataatg
     7621 attttgctgc cgttttcaaa ggtaatggtc agtacgccgc cgttgatttc gcagtcgata
     7681 tcgctgtcgc catcccagtc gtccaggcgt tcttcaatgg tcagccagag ttgatcagcc
     7741 aggcgatgaa attcactgtc gttcattgtt gtatcctgtc tttaagtgat ggcggcagta
     7801 tagcggtatg gggtcagggc ttcaaagttt gcccgtctgt tgctgcgttc cggcatgagt
     7861 catccgtcaa cggaataata atgtctctgt tgtgaagttc acgcacattc tcttctctga
     7921 cgtgtgtttt cgccgggcag ggggagggcg taatccttac ctctgtatcg gtccatgtat
     7981 aaacattgcg atcgtcataa gcgaagcgat cgtttttagt tagcggaccg ttaactggaa
     8041 taatttgcac gctctttgac cgtaattggc ggataaaata agcatgctgt ttgccgctat
     8101 taaatgagcg atcaatgacc aggtctggcg tatccagcgg gattatctca agctggtctt
     8161 caccaaatgt cgataatttt gttacataca gccgatcttt atattgcgca atattgccag
     8221 taatcagatg gaattgttca ggttcaatat ctggtaggtc tatccacttg ctccactgac
     8281 ggtcggacgt gagctgttta cgccagcgaa cgcgactttc ccctacttca aaagagacgt
     8341 catcatctac gggtatcgct ttgccagaca attgcccaaa gggataacga tgcataccct
     8401 tattgtcgcg aaaaaccagc agtgaatgag gtatccagcg aattatctca aatgagtctg
     8461 cattcgccgt aaggtgaacg ccatttaaat aaatattctg cccactgcgg ataagcagat
     8521 cattggaata caaatttctg tttacactca taagacgtgg attaataccc caccatcttt
     8581 tttctaatac agtaacatta ctgctgctcc cctgacgtcg accggacaaa tagagatttt
     8641 tactatccac cagcgtggta gagttacctt cgatagcttt tagtgtcgcc agatcaacac
     8701 gtgacgcgcc gctattactt tcagtgcgct gtccgtcaaa atagaggcta tatttatcga
     8761 ccgcgaagcg gcccatagcc tgaaagctgg caatatcgac agtgggtgtt cccggtggat
     8821 tgctaacgat tttgccacgc cagagaataa cgcggccgtc tgtcacccag tggtattcct
     8881 cattttttat gcttatttca tcggtgggta tatagccttc caccaactcc cgctccccgg
     8941 atatatttgt cacaaggcgc aaatcggccc tgctcatctc cggaaactgg gcaatgattt
     9001 tcttatcccc ccaataacct ttaccgtaag acataacacc gtgatcattg taatagtcga
     9061 accctatggc ctgtacagca ggcgtcagaa atgtgtgtcc caacaggatg agtgcaactg
     9121 tgattatttg acccttcatt ttgtcgcctc attgcgcggg gcaggaataa attcgccgtc
     9181 atcagtaatt tttccgtcca gatatcccgt tttgtaattt acgccatagc catatcccga
     9241 atgttgttcc ttccagatta agcgaacttg tttcggatca gcggtgcggt atcgccagag
     9301 ctgcgcgcca tcaaagctgt aaatgtagcg gttatcgcgg gcataccatc tgcgcaaaaa
     9361 gggatggtca ttaagatggg cttcgcgttc atccggagaa cggttatcca gtaagatcag
     9421 cggtccgtca gattcaaaca catgtaaacc ggaatggtat ggccaccctt cggcacgcgt
     9481 aagtaaatag ccgtggcgtt tcccggcgtt gaaacgtttg ttgataataa gattgggatc
     9541 atcaagtttc acgatttcca gcttgcggtc accagactct gtttcgatga tggtataaag
     9601 cgagtcctga tactgggcga cagcatcact gatgggttga aactgctccg gatcgagtcc
     9661 gggtaattct tcctgtttac aagcaggtcc tttacgccag aacactttct tttccagcag
     9721 agaaaaatcc agacagtgct ttgttaaatc ttcatcccac tccagatttt ccttgtcgag
     9781 gactttacgc tgcagcccgt ttttatcacg ccaggtcaag agtgagccgg gcatccagcg
     9841 cacgacggaa aaggtgtccg ggtcggcatc aagctgttcg ccgttgatca ggattttggt
     9901 ccgtgtacga gccagggtat cccaggggcc aaatggcata gccacacagg gattaaacgc
     9961 ggtagaaaac tttcctcgct gatcccatga cttttgtgcc agcacacgga aactatcggg
    10021 atctgcgagg cgatgcccat tgatatacaa gaaattagcg tcacgtaata tcagccccag
    10081 taagtcagga tcccagggag gccggaattc cacctgatgc agtgttttga tatctaagcg
    10141 atttcccccg ccattgttat cggtgcgctt gccttcgaaa tagagactgt gtttatctgc
    10201 ggcaaaatca ccccacgcct tgaacgaggc gacatcgacg ggaggtttgt ctggcgggtt
    10261 ttgcacaact tcacccgcgt agaggatata gcgaccatcg ctatgccagg agtaatgagg
    10321 ataccaggcc tgtgcgcctt catagaggta gtcggttgtc agatcagtca gaggaggtac
    10381 tttatcccat ttgtctagtg ttgtcgggtc tattaataaa ttgggtaaac ggcgcaaact
    10441 tttgtaattc acgtcaggaa gagaaagatt tgtttcttta taagggcgca ggtataccac
    10501 gccttttccc tgtttctggt aaatattatg ttcaggcgga tcaagccgac atccccatgc
    10561 cgacgataaa aacaatgaac aagacaacaa acaaaccaca ttgcgatagt gcataaagcc
    10621 atcctggcgc gaggtgccga tcacgaaact accagcagaa cataattcct cgcacgtaag
    10681 cgtaactttt gcggcaaatc gtcacttttc agaatctata aaggccatcg ctattttctg
    10741 atagttgctg gggtaaactt gaggttttca ttcacgatat aaatacgagc tactgcgcca
    10801 tggtgtgact gatcacgtta aaatgtgccg gattgacgcc gggaagggtg tcaacatgcg
    10861 actctgccca ccgttaccta ctttattgtc tttcgcgtgt tgtacttagg tcccaccacc
    10921 ggtatctaac attccccaca ttattacgct ttctgccgaa cgtgattttt atagaaatct
    10981 atcaattcat tttccctgta ccataaagtt tatctttcac atcctgccga acgcgggtct
    11041 tgtacccccc gttgtgaaga tcttcatacg gagaaaaaga atctttactc caccccagca
    11101 gcactttatc gccaggcata aaatgcacat gcagaaaatc ttctccccat tctcttttgg
    11161 ggagaggtaa cgtcatattc cgtttctcca gacgcattcc agcattatac tgtgtacgag
    11221 tagtacttaa tgtccagata acctctgccg tatccccact aacatcacca caacaggttg
    11281 ttgaaccgcc accatttgca aaagcattcg cgcccgccac gccgttaaca ctgaatccca
    11341 gtatcgggcg gtcaatttca ctgtggattt ccagaatcac tccacctctt ggtcctcccc
    11401 atactgcctg tcctatattc cacaacacga ccagtaccgg aacgacaatt aacgtccgcc
    11461 atacccagcg cccccaccgg gcataaccgc gcgtaagctt ttggtcaaat ttttccagtt
    11521 gggtaaatag ccccatcaga ttttattccc tcttatcacc aggttggcaa agttgtatgt
    11581 cactggcgac taaggtcaga caacaaaatt gcatggtgcg ccactctttt aaaagctcca
    11641 tgccgacgat aagaacaacg aacaagccaa caaacaaact acattgcgat agtgcataga
    11701 gccatcctgg cgcgaggtgc cgatcacgaa actaccagca aaacataaat ccccacgagt
    11761 aagcgttata ctcgcagcat ttcctcactt ttcagacttc ataaagagtc gctaaacgct
    11821 tgcttttacg tcttctcctg cgatgataga aagcagaaag cgatgaactt tacaggcaat
    11881 ccataatgaa aaacgtgttt aaggcactca ctgtattaat tactctcttc agcctgacgg
    11941 gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga taaaaacgca ccgccgccga
    12001 ccaaaccggt agagacgcaa acgcaatcca cggtgccgga taaaaacgat cgcgccactg
    12061 gcgatggtcc atcccaggtg aattactaaa agtcagtttc tgtacccgcg tgattggagt
    12121 aaatgatgca gttctcgaaa atgcatggcc ttggcaacga ttttatggtc gtcgacgcgg
    12181 taacgcagaa tgtctttttt tcaccggagc tgattcgtcg cctggctgat cggcacctgg
    12241 gggtagggtt tgaccaactg ctggtggttg agccgccgta tgatcctgaa ctggattttc
    12301 actatcgcat tttcaatgct gatggcagtg aagtggcgca gtgcggcaac ggtgcgcgct
    12361 gctttgcccg ttttgtgcgt ctgaaaggac tgaccaataa gcgtgatatc cgcgtcagca
    12421 ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga tctggtccgc gtaaatatgg
    12481 gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc taacaaagcg gaaaagacct
    12541 atattatgcg cgccgccgag cagacaatct tatgcggcgt ggtgtcgatg ggaaatccgc
    12601 attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt agaaacgctt ggtcctgttc
    12661 tggaaagcca cgagcgtttt ccggagcgcg ccaatatcgg ttttatgcaa gtggttaagc
    12721 gcgagcatat tcgtttacgc gtttatgagc gtggggcagg agaaacccag gcctgcggca
    12781 gcggcgcgtg tgcggcggtt gcagtaggga ttcagcaagg tttgctggcc gaagaagtac
    12841 gcgtggaact ccccggcggt cgtcttgata tcgcctggaa aggtccgggt cacccgttat
    12901 atatgactgg cccggcggta catgtctacg acggatttat tcatctatga agcaaccagg
    12961 ggaagaactg caggaaacac tcacggagct tgatgaccgg gcggttgtcg attatctgat
    13021 taaaaatcct gagtttttta tccgtaatgc gcgcgcagta gaagcgatac gtgtgccgca
    13081 tccggtacgc ggcaccgttt cgttggtcga gtggcacatg gcccgcgcac gtaatcatat
    13141 tcatgttctg gaagagaaca tggcgctgtt gatggaacag gctatcgcca acgaaggcct
    13201 gttttatcgc ctactctacc tgcagcgcag tctcaccgcc gccagcagtc tcgacgatat
    13261 gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg gcaggtgcga gtctgcgcct
    13321 gtttccggat cgctggcgct taggtgcgcc gtcgaaccac actcatctgg cattaagccg
    13381 tcagtctttc gaaccgctgc gtattcagcg tttggggcag gaacagcact atcttgggcc
    13441 gcttaacgga ccagagctgc tggtggtgct accggaagcg aaagcggtgg gatcggtggc
    13501 gatgtcgatg ctgggaagcg atgctgattt gggtgtcgtg ctgtttacca gtcgcgatgc
    13561 cagtcactat caacaagggc aaggaacgca gttacttcat gaaattgcgc tgatgttgcc
    13621 ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt tacacaccga tgtagaacgc
    13681 tacctacgtt atctgagcgt ggagcgccag cttagcccga taaccctgct taactaccag
    13741 cgtcagcttg aggcgatcat caattttgcc agcgaaaacg gcctgcaaag ctggcagcaa
    13801 tgtgatgtga cgatggtgcg caattttgct gtacgcagtc gccgtaaagg gctgggagca
    13861 gcaagtctgg cgttacggct ttctgcgcta cgtagctttt ttgactggct ggtcagccag
    13921 aacgaactca aagctaaccc ggcgaaaggt gtttcggcac cgaaagcgcc gcgtcatctg
    13981 ccgaaaaaca tcgacgtcga cgatatgaat cggctgctgg atattgatat caacgatccc
    14041 ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg gcgcgggtct gcgtctttct
    14101 gagctggtgg ggctggatat taaacacctc gacctggagt ctggtgaagt gtgggttatg
    14161 gggaaaggca gcaaagagcg ccgcctgccg attggtcgca acgctgtggc gtggattgag
    14221 cactggcttg atttgcgcga cctgtttggt agcgaagacg acgcgctttt tctgtcgaaa
    14281 ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct ttgccgaatg gggcataaaa
    14341 caagggctga ataatcacgt tcacccacat aaattacgtc actcgttcgc tacgcatatg
    14401 ctggagtcga gcggcgatct tcgtggtgtg caggagctac tgggtcatgc caacctctcc
    14461 accacgcaaa tctatactca tcttgatttt caacaccttg cctcggtgta cgatgcggcg
    14521 catccacgcg ccaaacgggg gaaataatgc gtttttaccg gcctttgggg cgcatctcgg
    14581 cgctcacctt tgacctggat gatacccttt acgataaccg tccggtgatt ttgcgcaccg
    14641 agcgagaggc gcttaccttt gtgcaaaatt atcatccggc gctgcgcagc ttccagaatg
    14701 aagatctgca acgcctgcgc caggcggtac gggaagtgga acccgagatt tatcacgacg
    14761 tgacgcgctg gcgttttcgt tcgattgaac aagcgatgct cgacgccggg ctgagtgccg
    14821 aagaagccag tgcaggcgca cacgcagcaa tgatcaactt tgccaaatgg cgcagccgaa
    14881 tcgacgtccc gcagcaaact cacgacacct taaaacagct ggcgaagaaa tggccgctgg
    14941 tggcgatcac caacggtaac gcccagccgg aactgtttgg tttgggggat tattttgagt
    15001 ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt cagcgatatg tactttctgg
    15061 ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca tgttggggac gatctcacca
    15121 ctgacgtggg tggggcaatt cgcagcggaa tgcaggcctg ttggatcaga ccggaaaatg
    15181 gcgatctgat gcaaacctgg gacagccgtt tactgccgca tctggaaatt tcccggttgg
    15241 catctctgac ctcgctgata taatcagcaa atctgtatat atacccagct ttttggcgga
    15301 gggcgttgcg cttctccgcc caacctattt ttacgcggcg gtgccaatgg acgtttctta
    15361 cctgctcgac agccttaatg acaaacagcg cgaagcggtg gccgcgccac gcagcaacct
    15421 tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta ctggtgcatc gtatcgcctg
    15481 gctgatgagc gtggaaaact gctcgccata ctcgattatg gcggtgacgt ttaccaacaa
    15541 agcggcggcg gagatgcgtc atcgtatcgg gcagctgatg ggcaccagcc agggcggcat
    15601 gtgggtcggc accttccacg ggctggcgca ccgtctgctg cgtgcgcacc atatggacgc
    15661 caatctgccg caggattttc agatcctcga cagcgaggac cagctacgcc tgctcaaacg
    15721 cctaatcaag gcgatgaacc tcgacgagaa gcagtggccg ccgcgccagg caatgtggta
    15781 catcaacagc cagaaagatg aaggcctgcg tccacatcat attcaaagct acggtaatcc
    15841 ggtggagcag acctggcaga aggtgtatca ggcgtatcag gaagcgtgtg accgcgcggg
    15901 cctggtggac ttcgccgagc tgctgctacg tgctcacgag ttgtggctta acaagccgca
    15961 tatccttcag cactatcgcg aacggtttac caatatcctg gtggacgaat tccaggatac
    16021 caacaacatt cagtatgcgt ggatccgcct gctggcgggc gataccggca aagtgatgat
    16081 cgttggcgat gacgatcagt caatctacgg ctggcgtgga gcgcaggtgg agaatattca
    16141 gcgtttcctc aatgatttcc ccggtgctga aactatccgt ctggagcaga actaccgctc
    16201 taccagcaat attctgagcg ccgcgaacgc cctgattgaa aacaacaacg ggcgtttggg
    16261 taaaaaactg tggaccgatg gcgcggacgg tgagcctatt tccctctatt gcgcttttaa
    16321 cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa acctggcagg acaacggcgg
    16381 ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc cagtcgcgtg tgctggaaga
    16441 agcactgttg caggcgagta tgccgtaccg tatttacggc gggatgcgct tcttcgaacg
    16501 ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt gccaaccgta acgacgacgc
    16561 ggcctttgag cgtgtggtga atacgccaac gcggggtatt ggtgaccgga cgctggacgt
    16621 ggtacgtcag acatcgcgcg atcgccagtt aacactctgg caggcatgtc gtgaactgtt
    16681 gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta cagcggttta tggaactgat
    16741 cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat gtacagactg accgggtaat
    16801 taaagactcc ggcctgcgta ccatgtacga gcaggagaag ggcgaaaaag gtcagacgcg
    16861 tatcgaaaac ttagaggaac tggtgacggc aacgcgccag ttcagctaca acgaagaaga
    16921 cgaagattta atgccgctgc aggcgttcct atcccatgcg gcgctggaag caggcgaagg
    16981 gcaggcggat acctggcaag atgcggtaca actgatgacc atgcactcgg cgaaaggtct
    17041 ggagttcccg caggtgttta tcgtcggtat ggaagagggc atgttcccaa gccagatgtc
    17101 gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg gcctacgttg gcgtaacccg
    17161 cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc cgtctgtatg gcaaagaggt
    17221 ttaccatcgc ccgtcgcgct ttattggcga gctgccggaa gagtgtgtgg aagaggtgcg
    17281 cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgg atgggtacgc cgatggtcga
    17341 gaacgacagc ggctacaagc tcggccagcg cgtacgccac gctaagtttg gtgaaggcac
    17401 cattgtcaat atggaaggca gcggtgagca tagccgtttg caggtggcat ttcagggcca
    17461 gggtattaaa tggctggtgg cggcttatgc ccggctggag tcggtgtaac gttgccggat
    17521 gcggtgctgc gcaccttact tggcctaaaa aatcattcag attcaataaa ttgcaacgtc
    17581 atgtaggccg gatagggcgt ttacgccgca tccggcatct gcgccatctt cagtatctga
    17641 cacaaaacta tcgttttaac ctttccgctc tacggaaatc atggtgacaa aaggatagcg
    17701 ttgccacggg attgccccgc ctttcatata catatgtgaa atcgtgccat caagataaag
    17761 cagctgctca acgttcagtt tcgctttggc ataacaggca aaatcataaa aatttgtcgc
    17821 ctgctggctc aacaaaaaca cggcattccc atgtttatta atcccaacac cgttacgaat
    17881 tttacgtgag gcgacgttgg gatgaatacg cggattaatt acactgtttt ccatcaacat
    17941 tggccctgac tgcaccgcaa actgaatctc tttactggtt ttgaaggcat ccagacgaac
    18001 gatgccgact ttatctcccg cgacataaaa cacgccgcca ggacggataa agaaattccc
    18061 ttcacctgaa gcgagattta acgccacctt ctgctgaccg ttttcgatgt acaaaccgag
    18121 cggcgcatag ctttcatcat agatgccgcc gttcatcgcc atctgcacct gaccctgact
    18181 attcatatcc gccagcagag catgtaaagt tccccacgct tcgccattgg ctttttgcca
    18241 gtacattttc acccgctctg tttgaggatt aacggtatac gcctgtacgg tcagcgtcgg
    18301 atctgagagt gcgcaatcat cagcggcaac agcaaacaag ggaagtaagg tgagggtgag
    18361 aaaaatccgt ttgagattca aggtgatcat tcctttacca atgagtagcc gatgcgccat
    18421 tataggtcct ggatgttgga tttttttatc ctgttagcga ccttgacgag taccaaaaag
    18481 cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg ttgacggcaa aattttgctg
    18541 gcgtaacatg cgcgcacgat cactctaaga ggacattcgc cttggacaca cccagtagat
    18601 actggctcac tatcctgtca tccaggatca actcctaagg ctatcccttt ttgctgatag
    18661 ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc tgagtcaggc tgtttaatgg
    18721 tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc cgatatttta agcattggga
    18781 gtcccggtca tgctgagcgc atttcaactg gaaaataacc gactgacccg gctggaagtc
    18841 gaagagtcac aaccccttgt aaatgcagta tggattgatc ttgtcgaacc ggacgacgac
    18901 gagcgactgc gcgtacaatc tgaacttggc cagagcctgg caacccgccc ggaactggaa
    18961 gacatcgaag catcggcacg tttctttgaa gacgacgacg gcctgcatat tcactccttc
    19021 ttcttctttg aagatgcgga agatcacgcc ggtaactcca ctgtggcatt taccatccgt
    19081 gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg cttttcgtct gtatcgtatg
    19141 cgtgcccgta gccagtcgat ggtagacggt aacgcctacg agttgctgct ggatctgttc
    19201 gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata tctatagcga cctggagcag
    19261 ttgagccggg tgattatgga agggcatcag ggcgatgagt acgacgaggc gctctccacg
    19321 ctggcggaac tggaagatat cggctggaaa gttcgcctgt gtctgatgga tacccagcgc
    19381 gcgctcaact tcctggtacg caaggcgcgt ttaccgggtg ggcaactgga gcaggcgcgt
    19441 gaaatcctgc gagatatcga atccctgctg ccgcataacg aatccctgtt ccagaaggtg
    19501 aacttcctga tgcaggcggc gatgggcttt atcaacatcg agcagaaccg catcatcaaa
    19561 atcttctcgg tggtatccgt ggtgttcctg ccaccgacgc tggtggcttc cagctacggg
    19621 atgaactttg aatttatgcc agagttgaag tggagtttcg gctacccagg cgcgattatc
    19681 tttatgatcc tcgcgggcct ggcaccgtat ctgtacttta agcggaagaa ctggttgtaa
    19741 tatgagtgcc ggataaagct atttttttat ccggcttatt tctaaaaatt gtctttttta
    19801 aagtcatgcg gattgaaaac tcgctctatt ttattttctt gtgttaa
//
LOCUS       DAZFDP010000084        19733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFDP010000084 DAZFDP010000000
VERSION     DAZFDP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..237
                     /locus_tag="ACHJ1G_003057"
     CDS             <1..237
                     /locus_tag="ACHJ1G_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO4764871.1"
                     /translation="GSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA"
     gene            410..2620
                     /gene="katP"
                     /locus_tag="ACHJ1G_003058"
     CDS             410..2620
                     /gene="katP"
                     /locus_tag="ACHJ1G_003058"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO4764872.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2664..3053
                     /locus_tag="ACHJ1G_003059"
     CDS             2664..3053
                     /locus_tag="ACHJ1G_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4764873.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3167..3934
                     /locus_tag="ACHJ1G_003060"
                     /pseudo
     CDS             3167..3934
                     /locus_tag="ACHJ1G_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3447..3557
                     /locus_tag="ACHJ1G_003060"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(4014..4409)
                     /locus_tag="ACHJ1G_003061"
     CDS             complement(4014..4409)
                     /locus_tag="ACHJ1G_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4764874.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4409..5368)
                     /locus_tag="ACHJ1G_003062"
     CDS             complement(4409..5368)
                     /locus_tag="ACHJ1G_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4764875.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5641..6543
                     /locus_tag="ACHJ1G_003063"
     CDS             5641..6543
                     /locus_tag="ACHJ1G_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4764876.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6540..6851
                     /locus_tag="ACHJ1G_003064"
     CDS             6540..6851
                     /locus_tag="ACHJ1G_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764877.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6927..7610
                     /locus_tag="ACHJ1G_003065"
     CDS             6927..7610
                     /locus_tag="ACHJ1G_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4764878.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7610..7828
                     /locus_tag="ACHJ1G_003066"
     CDS             7610..7828
                     /locus_tag="ACHJ1G_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764879.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7788..8274
                     /locus_tag="ACHJ1G_003067"
                     /pseudo
     CDS             7788..8274
                     /locus_tag="ACHJ1G_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8319..8558
                     /locus_tag="ACHJ1G_003068"
                     /pseudo
     CDS             8319..8558
                     /locus_tag="ACHJ1G_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8553..8801
                     /gene="psiB"
                     /locus_tag="ACHJ1G_003069"
                     /pseudo
     CDS             8553..8801
                     /gene="psiB"
                     /locus_tag="ACHJ1G_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8798..9517
                     /locus_tag="ACHJ1G_003070"
     CDS             8798..9517
                     /locus_tag="ACHJ1G_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4764880.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            9514..10111
                     /locus_tag="ACHJ1G_003071"
                     /pseudo
     CDS             9514..10111
                     /locus_tag="ACHJ1G_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10573..11073
                     /locus_tag="ACHJ1G_003072"
     CDS             10573..11073
                     /locus_tag="ACHJ1G_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO4764881.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(11075..11695)
                     /locus_tag="ACHJ1G_003073"
     CDS             complement(11075..11695)
                     /locus_tag="ACHJ1G_003073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764882.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            11801..12235
                     /locus_tag="ACHJ1G_003074"
     CDS             11801..12235
                     /locus_tag="ACHJ1G_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO4764883.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12328..12594
                     /locus_tag="ACHJ1G_003075"
     CDS             12328..12594
                     /locus_tag="ACHJ1G_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764884.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            12659..13549
                     /locus_tag="ACHJ1G_003076"
     CDS             12659..13549
                     /locus_tag="ACHJ1G_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO4764885.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            13549..13794
                     /locus_tag="ACHJ1G_003077"
     CDS             13549..13794
                     /locus_tag="ACHJ1G_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764886.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(13825..14076)
                     /locus_tag="ACHJ1G_003078"
     CDS             complement(13825..14076)
                     /locus_tag="ACHJ1G_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4764887.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(14110..14305)
                     /locus_tag="ACHJ1G_003079"
                     /pseudo
     CDS             complement(14110..14305)
                     /locus_tag="ACHJ1G_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14655..15503
                     /locus_tag="ACHJ1G_003080"
     CDS             14655..15503
                     /locus_tag="ACHJ1G_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4764888.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(15589..15924)
                     /locus_tag="ACHJ1G_003081"
     CDS             complement(15589..15924)
                     /locus_tag="ACHJ1G_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO4764889.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            16156..16488
                     /gene="mobA"
                     /locus_tag="ACHJ1G_003082"
     CDS             16156..16488
                     /gene="mobA"
                     /locus_tag="ACHJ1G_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO4764890.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            16500..19220
                     /locus_tag="ACHJ1G_003083"
     CDS             16500..19220
                     /locus_tag="ACHJ1G_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO4764891.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(19441..>19733)
                     /locus_tag="ACHJ1G_003084"
     CDS             complement(19441..>19733)
                     /locus_tag="ACHJ1G_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO4764892.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4730 a         4719 c         5415 g         4869 t
ORIGIN      
        1 gggagtacag gtgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa
       61 gcggaggtaa tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccacactc
      121 acgtgggtgg actggtataa caatcgacga ttgctgggaa ggctgggtca tatccctccg
      181 gcagaagcag aaaaagctta ttatgcttcc atcagaaacg atgatctggc agcctgagtt
      241 cacagataaa acactctcca ggaaaaccgg ggcggttcag ttgcggcttg gaacccctta
      301 gtattttttg tctgtagtat ctatcccagc aataggtata tcctgttgca tcaataaagt
      361 tgacttttgt atacaacatg cgaatttccc ttaatccgga gctattcgta tgataaaaaa
      421 aactcttcct gttctgattc ttctggcgct atcggggagc ttttctaccg ctgtagccgc
      481 tgataaaaaa gagactcaaa atttctacta tccagaaaca ctggatttaa ctcctctgag
      541 attacacagc cctgaatcaa atccctgggg ggctgatttt gattatgcca ccagatttca
      601 acagctggat atggaggctc tgaaaaaaga tatcaaagat ttgctgacaa cttcccagga
      661 ttggtggcct gcggattatg gtcattatgg tcctttcttt attcgtatgg cttggcacgg
      721 tgccggaaca tacaggacat atgatggccg gggaggcgcc agtggtggtc agcaacgttt
      781 tgaaccgctg aacagctggc cggataacgt taatctggat aaagcccgtc gattgctgtg
      841 gccagtcaag aaaaaatacg gctccagtat ttcctgggga gacctgatgg tcctgactgg
      901 taatgttgcc cttgaatcca tgggatttaa aacgctggga tttgctggcg gaagagaaga
      961 tgactgggag tcggacctgg tatactgggg gcctgacaac aagcctcttg cagataaccg
     1021 ggataaaaac gggaaacttc agaaacctct tgccgccacg cagatgggac ttatttatgt
     1081 caatcctgaa ggccccggtg gaaaaccaga tcctctggct tccgcgaaag atatcaggga
     1141 agctttttca cgtatggcca tggatgatga ggagactgtg gccctgatcg cgggagggca
     1201 tacatttggt aaagcacatg gtgcagcgtc tcctgaaaaa tgtattggcg cagggcctga
     1261 tggtgcacct gtggaggagc agggactggg atggaaaaat aaatgtggta caggaaacgg
     1321 caaatatacc atcaccagtg gcctggaagg agcctggtcg acatcgccaa cccagttcac
     1381 aatgcagtat ctgaagaatt tatataaata tgaatgggag ctgcacaaga gtcctgccgg
     1441 tgcttatcag tggaagccta aaaaagcggc aaatatagtt caggacgcgc atgatccgtc
     1501 tgtcctgcat ccgttgatga tgtttacgac ggatattgct cttaaagttg atcctgaata
     1561 taagaaaata accacccgtt tcctgaatga tccaaaagct tttgagcagg cattcgcaag
     1621 agcatggttt aaactgaccc accgggatat gggaccggca gcccgatatc ttggtaatga
     1681 agttcctgca gaatcattta tctggcagga tcctcttcct gcggcggatt atacaatgat
     1741 tgatggtaaa gacattaagt cgctgaaaga gcaggttatg gatttgggta tccctgcatc
     1801 tgagctgata aagacagcct gggcttcagc ttccacattt cgtgtgactg attatcgtgg
     1861 gggaaataat ggtgcccgca tcaggttaca gcccgaaatt aactgggaag ttaatgagcc
     1921 tgaaaaactg aagaaagtac tggcatccct gacctcatta cagcgtgaat ttaacaaaaa
     1981 acagtctgac ggaaagaaag tgtcgttggc tgatttaatt gttctttcgg gtaatgctgc
     2041 aatcgaagat gcggccagaa aagccggggt ggaacttgag attcccttta ctccgggaag
     2101 aactgacgcc tctcaggagc agacggatgt tgcctcattc agtgtactgg agccgacagc
     2161 agatggattc agaaattatt actcaaaaag cagaagtcat atatcgccgg ttgaaagcct
     2221 cattgataaa gccagtcagc tggatctcac cgttcctgaa atgacggcat tactgggtgg
     2281 tctgcgggta atggatatta atacaaataa ttcttcgttg ggagtgttta ccgatacccc
     2341 tggtgttctg gataacaagt tttttgttaa tctgctggat atgtcaacac gatggagtaa
     2401 agcagataaa gaagatacat acaatggatt cgatcgtaaa acgggagcat taaaatggaa
     2461 agcatcctct gttgatttaa tcttcagttc aaatcctgaa ttacgtgcgg tggcagaagt
     2521 atatgcctcg gatgatgcga gaaataagtt tattcatgat tttgttaaat cgtggaataa
     2581 agttatgaat agcgatcggt ttgatttaaa caataaatga taaaactgat gagggagggc
     2641 acctccctca tgaaggagaa attatgcaga aaagcaataa agttataatc agctgtggta
     2701 tggcattata ttttttgtgt ggggggatta cagtggcaga agaacttgat gaaagcatgc
     2761 aagttcttgc aaggaatatg agtgtacttc agtcatcaat ggataaaaag gagcttcttg
     2821 aagctcttga cagaatggat gatgctgtag atgagtcaat gaagcgtctt cctgagagca
     2881 tttcaccggc tgatgaagaa gggaagaatg actatataaa agaacttcaa aagcttgaaa
     2941 aagagattat gctggcgaaa caaaaagtct tatcgggaca gttatcagat atacccgatt
     3001 cggtaaacaa gatgaataaa atcagagttg aggggcatga taaattcaga taaatcagtt
     3061 ggttgattcc tgcgggacga atacatacgt caggaacata actttatggc agtgaaccgc
     3121 cccgggaatc ctggagacta aacttcctga gaaagaggta aacaggatga ctaaaaatac
     3181 tcgtttttcc cccgaagtcc gtcaacgggc gattcatatg gttctggaaa gtcagggcga
     3241 atatgactca caatgggcgg caatttgttc cattgcccaa agattggctg tacaccggag
     3301 actctgcgtg tctggggacg ccagcatgag cgggataccg ggagcggtga tggtgggctc
     3361 accagcgctg aacgtcagcg tctgaaagag ctggaacgtg aaaaccgtga actgcgccgc
     3421 agtaacgata tccttcgcca ggcttccgct tattttgcga aggccgagtt cgaccgcctc
     3481 tggaaaaaat aatgccactg ctgctgcgtg agcagtacgg ggtcggaccg gtatgcagtg
     3541 aactgcatat tgccccgtca acgtattacc actgtcagca acagcggtat catccttata
     3601 aacgtagtgc ctgtgcacag cgcgatgact ggctgaagaa agagatactg cgcttatacg
     3661 atgagaatca tcaggtatac ggtggcgtaa agtctgacgt cagttgttac aggaaggtat
     3721 cagggtggcc agatgtatag tggcgcgcct catggcggtt atgggacttg ccggtgttct
     3781 ccggggtaaa aaggtccgca ctaccgtcag ccggaaagct gttgccgcag gcgacagcgt
     3841 aaaccgccag ttcgtggcag aacgtcctga ccagctgtgg gtggcggatt ttacttacgt
     3901 cagcacatgg cagggcttcg tctgtatggc gttcgattta gggcactaaa accgctgctg
     3961 gtccctcata ataaaagaag agcaggtaat agacttaacc tgctcttctt ttattatttt
     4021 tttaacatag aaagattgcg tctggtttgt tcttcgaaat catcttttga agtgatgctc
     4081 tctgatttgt tttctgaaga tgcccctatg atgtttttta acacacttat atcaattgat
     4141 gtagatgtgt atcctgttgt ttgctctata agtttcacca gattttctgc agtgattttc
     4201 tcactgaaaa aaccactgat tagcgttaac agtcgggggt caacagaata aagagctgct
     4261 ccgcaaataa gagattgtct gtagaagtct ccccgcattt gtatcgggag agagtctatt
     4321 gtttgttgtg tcaggttatc ctgcggataa agtgtcgggt gcaggtatgc cacaactttc
     4381 tttctttttt tcggatcact gctttccatt acgcatgcac cattgctttc accaatgcaa
     4441 actgaggttc atccaccttg ttcactttca gattcgggaa atattgcttt atgtgagggt
     4501 aaataagctc ggctcctccg ccagtgagat agattcggtt cattcctgag aatgttgaaa
     4561 tttcctgcct gatgctttct gccagagatt tcacttccct gtctattaca tgagtaatgt
     4621 tttttatttc agctacatca tttattaatg tctggagata ttcattatca tgtctgtttt
     4681 ttattaactc atcagcaata aaataattac atggtgtgga ggctttcgtc agcgcattca
     4741 tgacggtatg agtgatgcgg gatgtcccga tttctgaata cccacggatt tcagaaatgc
     4801 cttcaaatgc accgagaatt aaaccacaat ccagagtggt tcctccaaga tcgataatca
     4861 ggctgcgttc aagtttgttt actttatctt tttttagtga ttcaaatgca gctggcaaag
     4921 attcaggcat tacattaact tttttaattt taaatgtttc ccctttattc agagatatct
     4981 cacggagaac atttgccttt tttctttcta ttctttcttc atttggttga ttgtcattat
     5041 caaagaattc ggttacaggt aatgtcactg tcagttctac atcctgtggc tgaaggccgg
     5101 atgtcagcag tgcatgatgt atggcaagca aattggtggt gctgtattga taactgacat
     5161 gtgttgtacc tatgacagca gtactgccca aatcataggt gtattttttt ccatccacga
     5221 cgtaattgta aactttgccg gcattaaaca gtcccgggtt ccagccttct ttaaagctgt
     5281 ttcctgacag actggttctt acctcgccat cttcaagcca ggccagtttt acgttagttg
     5341 aaccatcatc gcatgatact tttagcattt tttcttgcct cgttttgagt ttttatttat
     5401 ctcaaattga gcatccttta tctagatgca tctttttgag ttttttgttg ttccttattg
     5461 tgtctcattt tgggttcatg tcaagcgatg aggctctttt agagtctctt tgcattcttg
     5521 ttgtgactca gtttgagtct ctatcgattt ctcattttgc gtttcgaact gagacggaaa
     5581 ctacttaacc aggccgcatg tgagaggaga attcccatgc caaactggtg ctcaaatcgt
     5641 atgcattttt ctggtgaacc ggcacagatt gctgagatta aacgactggc cagcggtgcc
     5701 gtcacaccgc tttatcgccg cgccacaaat gaaggtattc agctgtttct ggccggaagt
     5761 gccggacttc tgcagaccac tgaagatgtg cggtttgaac catgccccgg actgacggct
     5821 gccgggcgtg gcgttgtatc gccggagaat atcgcgttca cccgctggct gacatacctg
     5881 cagaacggtg tactgctgga tgagcaaaac tgcctgatgc tgcatgaact ctggctgcag
     5941 agcggtactg gacagcgtcg ctgggaagga ttaccggacg atgtcaggga taccatcacc
     6001 gcacttttca ccgcaaaaag aggtgactgg tgtggcttct ggagtaacga ggatgtatcg
     6061 gtgtggtgga accgtctgtg tgacaacgta ctgccggaaa aaaccatgcc gtttgacctg
     6121 ctgacggttc tgccgacccg cctggatgtt gaagtgaatg gctttaacgg tggtgttctg
     6181 aacggtgttc cttctgcata tcactggtat acggaacagt atggcgtgaa gtggcctgtg
     6241 gggtatgagg tgaatatcag tagtcaggga gacaacttca ttcaggttga tttcgacacg
     6301 ccgtggtatc agccggaaag cgacgttatt gcagaattaa gccgccgttt cagctgcaca
     6361 ctggagcact ggtatgccga acagggctgc aatttctgtg gctggcagcg atatgagcgt
     6421 ggcgagcttg ttgatgtgct gtggggggag ctggaatggt cacatccgac agatgatgac
     6481 gagttgccgg aagtcaccgg acctgcgtgg atagtcgaca aggtggcgca ttatggcgga
     6541 tgaagtatga cggagacggg cgggcaactg cccgtttctt ttccggtaaa ggatgtcgcc
     6601 gtgctcctct ttttactgcg ccggctgacg cggcgcggca taaacgctgt ctgtggtcag
     6661 tgtcccgcgt ccggcgggca cgggacgggc gcttttaccg ctcccggctg gcgtcggtga
     6721 cagtgtacgc cagcccgtcg cccttttctg atgaacgccc ttcaagccgc tttcgcggca
     6781 tagccttgcc gtcaaaaaga cgaaggctgc ggtattccac ggtcggcctg acccgttacc
     6841 ggacgcggtg aacagcccac aggcagcggg taacgggcac cacaggggtg ccctcccggt
     6901 gccctctgta agagaaggag ttttgtatgt cccgttttgt cctcggtaac tgcatcgatg
     6961 ttatggcccg tattcctgat aacgccattg atttcatact gactgacccg ccgtacctcg
     7021 tcggtttccg tgaccgttcc gggcgcacca ttgccggtga cgttaatgat gactggctgc
     7081 aacctgccag caatgagatg taccgcgtac tcaaaaaaga cgcgctgatg gtcagcttct
     7141 acggctggca ccgtattgac cgctttatgg cggtctggat acgcgccgga ttcagcgtgg
     7201 cagggcacct cgtgttcacc aaaaactaca catccaaatc tgcgtatgtc gcgtaccgcc
     7261 acgaatgcgc ttacatcctg gcaaagggca gtccggcccg gccccgtaac ccgctgccgg
     7321 atgtgctggg ctggaaatat tcgggcaacc gccatcaccc gacggaaaag cccgttacca
     7381 gcctgcaacc gctgattgag agcttcacac acccgaactc aattgtgctg gacccgtttg
     7441 caggcagcgg ttcaacctgc gtggccgccc ttcagtccgg acgccggtat attggtatcg
     7501 agctgcttga acagtatcac cgtgccggac agcaacgcct tgccgccgtg caacgggcca
     7561 tgcagcaggg ggccgcaaat gataacaggt ttgaaccgga ggcagcatga tgaattacag
     7621 cggatttgaa gatatgcgtg aacaggtggc tgaactggcc aacaccatgt gcgacctgcg
     7681 cacaaccatg aatgagatgg agcagcggta cagcgatgca gacaccctgc cggaacgtct
     7741 ggtgcgtcag acgctctttc gtgccagcca cctgtttatg aaggcatata acgaaatact
     7801 ggaactggat tactgcttca aagattgagg ggaaagagta tgtacggaac atgcgaaacg
     7861 ctctgccggg cgctggcagc aaagtatccg ggagacacgc tgttgatgct ggttatctgg
     7921 tccccggaag agattcaggc cctcgctgac ggaatggata tttccctggc cgatcatgaa
     7981 atcaggacca tcctggcgcg cctggaggac atcccggaag accagcggac tgagtccggt
     8041 atttcttcct ccgcggcgat ggagattatc agcaacgtga gcgaaaaccg ccaggtgacc
     8101 gtccctgctg aactgctggc gtccctgatt cagaccgctg aacaggcatt gtggaagcgt
     8161 gaatgggccg cccgtgatta cggcctcgcc gtcccggaat gcgttacccg ccgtcaggcg
     8221 gtggttaatc aggcccgcat cctgctgaaa aacaacacgc acgaaaacga ctgatgttat
     8281 cgccgcctcc gggcggcgat tcagggagac catgaatcat gaataaaacg ctgaatgcac
     8341 tgatttgtcg tcatgcccgc gacctgctgc tggcgcaggg ctggccggaa gagacggatg
     8401 ttgaccagcg taacctgaag tatccgggct ggatcagcat ttacgtgcgg ctggatgcgc
     8461 cccggctggc gacgttactc gttaaccgcc acgatggcgt actgccgccg cacttggcct
     8521 ccgccattca gaaactgacc ggaaccgggg cggaacttgg tttcacgcca gcccacgaac
     8581 gtttccacct ggcattatgt tcgccgggag acgtctctca gctctggatg ctggttctgg
     8641 tgaattgtgg tggacagcct ttcgccgtcg ttcaggtgca acatatcttc acgcctgtcg
     8701 ctatcagtca cacgctggcg cttgccgcga cacttgatgc gcagggatac agtgttaacg
     8761 acatcatcca tatcctgatg gcagaaggag gtcaggcatg agcgcacgtt cacgggcact
     8821 gattcccctc agcgcagaac aacaggccgc catgcaggct gtggctgtca cagaacaacg
     8881 tcgtcgtcag ggacgcacac tttcagcatg gccttatgcc agcgctttct ttcgctgcct
     8941 gaatggcagt cgccggattt cgctgaccga tctccgcttt tttgcccctg cgctgacgaa
     9001 ggaggaattt catggcaacc gtctcctgtg gctggctgcc gtggataaac tgattgaaag
     9061 ttttggtgaa gtctgtgttc ttcccctgcc atccgatgcg gggcatcgtc tgttcccgtc
     9121 cgttcctttt cgtgaaggtg agcgtcgtcg tcagaaaacc acgctgacag agcagaaata
     9181 cagccgccag cgggaacgtg aggcagaacg acgggaactg gaataccaga catgttttgc
     9241 tcaggcgcag attgaccttg cgtttcatac tcccgccacg gtcggaagct ggttgtcccg
     9301 ctggtctggt gttgttgagg agcatgatct ggaaacgatt ttctgggggt ggtgcgggcg
     9361 ttttccatca ctgtcatcat ttgaccggtt tttctggcag gaggaaccac tctggcggct
     9421 gatttttgaa gccggtgagg ccggtcgtgg tgcaccggta caggtacgtg cacttgagca
     9481 gtggatgatc ccgaacaagc tggagaacgc aatatgatga aatcagacga acaatatcag
     9541 gttcccgtgt ggatgcgacc tctgttgccg ttgctctgca ataccggtgg taatgatccg
     9601 gaggaacttc tgaatgatac agaaaccacc gccagtgcga atattgtccg ttatgtactg
     9661 atagttgcag tgcggtcgca gattggatct gctgcagcta ctgtacagga aaggactgtt
     9721 gcgcacagag ataccaggtg gcttttcacc ggaagaagcg caggaactcc tggataatct
     9781 ggtgcgcagc catatcagca aggcgctgtc gggtgagcga atggcagccc gtgacagata
     9841 tgccgagctg gcctggattc gccagcaact ggtggatgcc gcctggtttg tccgtgccac
     9901 actggaagcg catggtacga gcgtcggaaa tgagagtccc tctgcacttc cagagacaat
     9961 gccggacata cagacacggg aactggtgat gctgatcaaa cgactggcat cttctctgaa
    10021 agcggtgaaa ccggacagta gcgtggtgcg tgcggcgcag gactggctgt gcgacagaaa
    10081 acttgtggat atcacagata ttctccggtg aaccaggcat gtatattttt cgcagggaca
    10141 cgccgatcat gatcgtttca tatcaggggt ggtctggagg tcatgcggcg tgtcctctgc
    10201 actcgccgga ataaggaagt cgccggcgga tccgctttta cccggccatg cggggcatgg
    10261 ccttgtgggt tttcagttat gtggcctcag cgtcgtgtgc gggctgtgcc gtgcctccat
    10321 cgtagccggg cttctgcggg cgggatgcaa ggtaagatga accgctgcgg tcacccggtc
    10381 cctccttccc gtctgccgtg attttccggt cgttcacccg ctcagatttc gcggtctcac
    10441 cctccaactc ccgtcagccg tgcgcgtcgg gctgcggctt cccttgcgcc cttgcatccc
    10501 tgcaggcccg gcaaagatgg aggcacggca ccgcccggtg tcgcagaaca tgtgactatg
    10561 gaggattcgg gaatgtctgt tgttgcacct gctgtatacg ttggaacctg gcataaatac
    10621 aactgtggaa gcatcgctgg ccgctggttt gacctgacca cgtttgatga tgaacgcgac
    10681 tttttcgccg cctgccgtgc tcttcaccag gatgaagccg atcctgaact gatgtttcag
    10741 gattatgagg ggttcccggt gaatatggcc tccgaatgcc atatcaactg ggcctgggtt
    10801 gaaggcttcc gccaggcacg ggatgaaggc tgcgaagagg cttatcgtct ctgggtggat
    10861 gataccggtg agacggattt tgacaccttc cgcgatgcct ggtggggcga ggctgacagt
    10921 gaggaggctt ttgcggttga gttcgtcagt gataccggcc tgctggctga cgtgccggag
    10981 acggtggcgc tctattttga ctatgaggcg tatgcgcggg atttattcct ggactccttc
    11041 acctttattg acggtcatgt gttccgtcgg tgatttactc ccccgctccg gcgggggtat
    11101 ggcctgccgg gccgccggaa aacccaggca ggcttacgcc tgcctgaaat acaggagggc
    11161 cgcgcctgcg gcgctgccct tcctgcccgt catggtgtga ggcatttttc tgttttgtcg
    11221 tttctgttgt tctgatgccc ggaggcgggg acacccttcg gctcccggct gcctggcggc
    11281 atccggtcgt cggcccgact ccgacaggca gctgctggcg cacccgccag tctccgccga
    11341 acccgctggc gcggtttcgg gctgccgcta cggtccgatg tctgaaaaac caggcctgca
    11401 gcgaccgttc cggtcgcgcc gcccctgtcg cgccggaaac ggcgctccct gcacacctga
    11461 cggtgtgtaa ggccactgcg cctgatggcg cagtcaaccc ttctgccgcc acccctgcgg
    11521 cctgtttccc gctgtggact ttccgggggc tgaagcggcc acaacacggg gcagccgtcc
    11581 aggggaagat gtaccagcca caaaactttt tgccgcaggc gtcaaaaact tttgcgtctg
    11641 cccccggaca actgccccgt gccgtggcag tcgctgcccc atcggaaagg tccactgcgg
    11701 ggagaaaccc cgaagaagtg gacggcagaa ggggctttcg cccctccggg ggagcaaccg
    11761 gtcggcccgg cgacctgatt cagcagaagg agacgttttc atgacagtca gcagcacgat
    11821 ttccgttttt tgccgcgacg gggtgttccg taccgtttac tgccacctgc acggggagcc
    11881 gacctggaac ggtcgcatcc tgcataccca ctatgccacc ggtcagctgg ccgaagccct
    11941 ggttgaacac ggagatatcc gttgcctcgg tccccgttgc gacaaatccg ccgggcatac
    12001 gcttcagaac ccggtggacg gtgtgacggc ttattacgga cgtgacagtg gtttccggat
    12061 ggacagtgag gcgcgtgagt accgttcttt catggaggct attgccactg aaagcactga
    12121 agaggtgcgc ttccattatg tgttcatcga cggctactgg aaggtgatgt accgcacgcc
    12181 ggaaggctgg aagatgaaag cgctcgcgct ggcactgcgc cgctgtccga aatgaaaaaa
    12241 ggcagggcga taaaccctgc ctttctccgc cggcgcttcc ccgtacggaa gtccggcatc
    12301 tcaatcacta tctatggaga ttatgccatg agaccatcca ttatcttcgc aaccgccgag
    12361 tatgtaaagc gtctgcgtga agagtgcctg cgggagaata aacccctgca ccgccatacc
    12421 cgcgtcagac gtcaggagct ggcacaggat gagattaacc cggacgtcct ggcgatgagc
    12481 ggtcatatcg ccagacgctg cagtgagcag aagcgggtac gtatccccgc catgaaagtc
    12541 agcgaatggg gccacctgct ccgcgcgctt gaaattgagc gggcctgcca ctgattaacc
    12601 cttgccgtct gcctgtgcag gcggcaggat acgttcagac atctcaccag gaggaaccgt
    12661 gagtaagaag aaaaacacca caacgcccac gccgcatgat gccgcgttct ggtcgttcct
    12721 ggcgaatccc gacgtcgcca gagattttct ggaactgcat cttccggcgg agtaccggca
    12781 gttgtgcgac ctgtccacgc tgaagctgga acccgccacc tttgttgagc cggacctgca
    12841 tcagtacgcc agcgatatcc tctggagtgt gaaaaccacc gggggtgaag acggctatgt
    12901 ttatacgctc atcgagcacc agagcaccga aaatctgtac atgcctttcc gcatgttacg
    12961 ttacagtgtg gcggcgatgc agagacatct ggagcagcac aaaacattgc cactggtgat
    13021 tccggtgctg ttctatcatg gtgagcgcag cccgtacccg tacagcatga actggctgga
    13081 ctgttttgag gaaccggcgc ttgcggctaa aatatacaca aagccgtttc cgctggttga
    13141 tatcactgtc gttgatgaca atgaaatcat gaaccatcgc cggatggccg cactgacgct
    13201 gctgatgaag catatccgcc atcgtgacat gatggagctg ctggacaaac tcccgcaggt
    13261 catggtggaa atttcagacg agcaggtgcg tgttctgatt cattacatcg ttaacgcagg
    13321 ggactctgta tcaccggaat ttatgcgggc gctggctgag cgtctgccgc agcatgagga
    13381 taaactgatg actatcgctg aacgtcttga gcaaaaaggt cgccaggaag gagcgcttga
    13441 aaaagccctg gctattgcgt gccagcttca gaaaatgggg atgacgccgg agcagattaa
    13501 gcaggctacc ggactttccg aagctgaact gaagaacatc actcactgat gagaaccaca
    13561 aagcccggca aaatgccggg tttttttgtc agcaggagca gagcaaacag catgacgatt
    13621 gcagaacgtc ttatacagaa aggtttcgac gaaggtttta agaaaggtgc gcttgaagtg
    13681 gcgcgggaag cagcctgccg gctgcgggat atgggctgga cgccggaacg gattcaggag
    13741 gcaaccggac tttccggtga agaactgaaa aagctgtttc ctgatgagca gtagcctggc
    13801 attctgccag gctgtgaatc acgctcacac attccctgcc gtttccccca gttcattttc
    13861 cagacgcctg atgatatctg ccttgcgcat cccctgcatg tgaggcagca gatcacggac
    13921 tgaaagtgcc tgggtggtcc tccagacggt gacatccacc tgttccgcat tttcccaggc
    13981 gtgccatgtt ctctgtgtca cgccaaattc tctggccgct ttttcgcgtg accagagcat
    14041 gctttttcgc cagattcgca gttcccagcc ggtcataaaa cacctttgaa aaagtgaaaa
    14101 aacttcactt tattttatca tcataatcac caggtcagcg cgataaataa gagtgaaaaa
    14161 gtgaagaaat ttcacttttt agttgtaggt gtcgtgctgt tttctctgtt ttcacctgtg
    14221 cgggagtggt gcgccagtga ccaccacatg ccattgttgt gctgtcgttt ttcttcagtc
    14281 gcgccggatg cggcgctccc tgcacacctg acggtgtgta aggccactgc gcctgatggc
    14341 gcagctgtct ctgcctgtct gccgccgcac tgcggcccgt tctccgcaga cggactttcc
    14401 gggggtacat acacgaccac atcccgtttc agtcgtccag gggagagatg taccagccac
    14461 aaaacttttt gccgcaggcg tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg
    14521 acgtggtgag cgtcccccat cggaaaggtc cactgcggga agaaccccct gtgaagtgga
    14581 cggcagaagg agcttcgctc ctccgggaaa tgaccaggct gacccggcag ccgtgacgat
    14641 gaggagacaa tatgatgaat cagactttac ccactgctga cctgaatact gccggcacga
    14701 cagatattat tccgtctatg gctatcgacc gcatcatcgc gcagcgtaac gaaggtattg
    14761 cactgttcat gcaggcgatg gaatgcctgg cgacagcgcg caggattctg ctcgatgctt
    14821 ccggtgacat ttttctctac gggtttgaag actgcgtgac tgactccgtt cgatgcatgg
    14881 ataaaccgga agaagcaaaa aagaatatca cccgtcttgc cgaccggaaa atctgggatc
    14941 gcctgatgac ttatacgggc atgtacacct tcatgagttc atgccagcgt gatgagtgga
    15001 acagccagct gatgagcgac acctgtcctg aaatcaccct ggacaatgta ctggcaacat
    15061 tccgccatct gaatgccagt aagatgcaga catttgaaca ggaactgatt gatatctggc
    15121 ggaaattgtc atgggattac agaaccaata atccctgccg tctgggcaag agaatcatta
    15181 ttgaaaacct gctgtaccgc tggagtaacg ggcgtgtgac gctggactgc agcggacggg
    15241 aggcactgga tgacctggta cgtccgtttt atctgctgga gggacgcaac gttcctgact
    15301 tcaggaacag catcggggcg cagtatggtg aatttctcgg gaacggcgac aatatcggtg
    15361 agttgtttga aggggaatat tttacggtgc gtggctacca gaaagggacc gtacacattg
    15421 tctttaagcg tcctgacctt gttgaaaaac tgaatgatat tattgcacgg cattatcctg
    15481 gtgtattgcc gccacgagtc tgattaaaca gaaaagccct ggtatttatg ccggggcttt
    15541 tttttctgta atcatcataa tctcagaata cgatgtgaca ttgtcacatc aattgttgtt
    15601 catatcgatg ccatctttcc tgcctgaatc tgtcttttta ggtgctgttt tagaggaaga
    15661 acggcgggat ggttttttcg attctgcttc ccgaataatg tcacgaagtg ccttctctcc
    15721 tacttcatac cctgcatttt tgagcgtgtc acgaacatct gccagtgtat aaccctttgt
    15781 ccttaccagg acaatgatgt catcacgaat ggcatcaaga aaatcacgca atgtttttcg
    15841 ttgagcggtg aggtccggca attctgataa agcagctttt gccagctgga tgtcatcatc
    15901 tgagtaaaat tttttagaag ccattggcac tttttctccg tttcatgttg aagtctgatt
    15961 ttatcatcaa aaactgcctc ctggcagagc tgcgccccgc ctctgccgtc tgcataagac
    16021 tatgatgcac aaaaataaca ggctataatg cctgaaaaac gggacaggat gtgtaatcgt
    16081 ataaccgcgc acgcacggct atacgattac cacctggtca gggcttagcc ccgacacccc
    16141 ggtaaggagc agaccatgag tgagaagaaa acccgaagtg gcagtgagaa acggcaaaag
    16201 aatgtcctca ttgctgttcg tttttcacca gaagaggctg agattgttaa agaaaaggca
    16261 gaaaaaaacg gactgacagt ttcaactctg ataagaaaaa ctgtgctggg aaaacagata
    16321 aatgcccgca ttgacgaaga ttttttgaaa gagctgatgc gtcttggtcg cctgcagaaa
    16381 catctgtttg tggaaggaaa acgcacggga gataaagagt atgcagaggt tctcgttgct
    16441 attacagagc ttgctaatac tctgcgaaga gatttaatgg gacgctgaga taatcgctga
    16501 tgaatgccat cattccgaaa aaaagacgcg acggaaagtc ttcttttgaa gacctgattg
    16561 cgtacacctc agtgcgggat gatgtgccgg aagatgaact caggccagac acggaaatga
    16621 aagctgagat tccgcaccgg aaccgcttca gacgtcttgt tgattatgca acacgtctgc
    16681 gtaatgaaaa atttgtttcc ctgattgatg tgatgaaaga cggcagtcag tgggtgaatt
    16741 tctatggtgt gacctgcttt cataactgtt tgtctcttga atctgcggct gaagaaatgc
    16801 agaaagcagc agaccttgct catttttcca aagatgatac agatcctgtt tttcactata
    16861 tactctcctg gcctgcgcac gaaagtccgc gttctgagca gctttttgac tgtgtgcgtc
    16921 acaccctgaa atcccttgag ctgtcaaaac atcagtatgt ggcagctgtt catacagaca
    16981 cggataacct gcatgtacat gttgcagtga atcgtgtcca ccctgaaacc ggctatatca
    17041 attgccttcc gtggagccag gaaaaactca gtcgtgcatg ccgtgaactt gaaattaagc
    17101 acggattcgc gccggataat ggctgctttg tgcatgcacc gggcaatcgt attgtgcgca
    17161 aaacagcgct ggtacgtgag cgccgtaatg cctggcgacg cgggaaaaag cagacgttca
    17221 gggaatatat tgttcagatg tcgattgccg ggctgcgtga agagcccgca caggactggt
    17281 tatctttgca taaacggctg gccagtgatg gtctgtatat cacgatgcag gaaggggaac
    17341 tggtggtgaa ggatggctgg gacagagcgc gggaaggtgt tgcactcagt tcgttcggac
    17401 cgttatggac agctgaaaaa ctggggcgga aactggggga atatcagcct gtaccgacag
    17461 atattttcag tcaggtgggg acacccggtc gttatgatcc ggaagccata aacgttgata
    17521 tccggccgga aaaagtggca gaaacggaga gtctgaaaca gtacgcctgc cgtcattttg
    17581 ccgaacgctt accggaaatg gcgagaaatg gtgagctgga aagttgcctg gatgtgcaca
    17641 gaactctggc aacggctgga ttatggatgg ggatccagca cgggcatctg gtactgcatg
    17701 atggatttga taaacaacag acaccagtac gtgcagacag tgtgtggccg ttaatgacgc
    17761 tggattatat gcaggatctg gatggtggat ggcagcctgt accgaaggat atttttactc
    17821 aggttatacc cggagaacgg ttccggggac gtaatcttgg tactcaggct gtcagtgatt
    17881 acgaatggta tcgtatgcgg atggggacag gtccacaggg agcaataaag cgcgaactgt
    17941 tttctgataa ggaaagtctg tggggatacg gccttattca gtgcaggagc ctgattgaag
    18001 agatgatagc tgacggggat ttcagctggc aggcatgtca tgagatgttt gcgcgtaaag
    18061 ggcttatgct gcagaaacag catcatggtc ttgtgattgt ggatgcgttt aatcatgagc
    18121 tgacaccggt gaaggccagc agtatccatc ctgatttgac gttatcccgt gccgagcctc
    18181 aggccgggcc atttgaaatt gcggctgcag atattttcga acgggtgaag cctgaatgtc
    18241 gttacaatcc ggaacttgct gccagtgatg aagtggaacc aggcttccgg cgcgatcctg
    18301 agattcgtcg tgagcgccgt gaagcccgtg cagctgcgcg ggaagatttg cgtgcgcgtt
    18361 atcttgcatg gaaggagcac tggcgtaaac ccgacttacg ttacggagaa cggttacggg
    18421 aaattcatgc tgcctgccgt cggcgcaagg cgtatatccg tgtgcagttc cgggatccac
    18481 aattgcgtaa gctgcattac catattgcgg aagtgcagcg tatgcaggcg ctgatcaggc
    18541 tgaaggagag tgtgaaggaa gagcggttgt cactgattgc ggagggtaag tggtatcctt
    18601 tgtcctaccg gcagtgggtg gaacaacagg cggctcaggg agacagggct gcagtatcgc
    18661 agttacgtgg ctgggattat cgtgatcgtc gctcgcgtaa taaggataaa agacgcacaa
    18721 ctaatgttga tcgttgtgtc gtactctgcg aacccggagg aacgccactt tttaataatg
    18781 ttgcaaaact tgaagctcgt cttcagaaaa atggaagtgt tcatttcagg gatacgcgga
    18841 caggaaaaaa tgtctgtacg gattacggag atcgggttgt gttctaccat cacacggacc
    18901 gaaatgaact ggcagagaag cttgatttaa ttgcgccagt tctgttcagc cgcaacggaa
    18961 agcttggttt tgagccggaa ggttcatacc agcagtttaa tgatgtgttt gcagaaatgg
    19021 tggcctggca caatgcagcg ggaataaccg gaaacggaca cttcaccatt actcgtccgg
    19081 acgtggattt gcaccgtcaa cgcagtgagc agtattaccg ggaatatatc agacagcaga
    19141 cccgtttatc ggtgagccat gatgataatt atacgttacg gcaggagaaa acatgggagc
    19201 caccatcacc tggaatgtaa gcccggataa gtgatggtgc aaagattcag cccaaaaacg
    19261 gcaggaacct tcgtcaataa gggcgggagt agtcactgaa ttgagtttat acccggttaa
    19321 ctctgaatta agggccgtgt ctgcggccct tatccagtgc gccgtaaaac cccggactta
    19381 gtgaacaggg ttttacagtc tgattgacag atatggtcac cagaaaatat ttgtattcct
    19441 tcagtatttt tcgtcagttg ttggttgtgc tccacatgat ttctggggat gatcattatt
    19501 tattgtctgc gatttattat tttcagcgac cggtaacagt atctccaggg ggagcagggt
    19561 attcagaatc gccttcactg aaggtatatc ttcaggatca gagtgttcct cttccggacg
    19621 attcagtgtg atataacata cccggcgttc ttccctggtt gtgttattga tagtgtgcac
    19681 agcctttttc cacagttcgt ttgcccggga ttcgatatct gtatcatatt gcg
//
LOCUS       DAZFDP010000085        19653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFDP010000085 DAZFDP010000000
VERSION     DAZFDP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..179
                     /locus_tag="ACHJ1G_003085"
     CDS             <1..179
                     /locus_tag="ACHJ1G_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764893.1"
                     /translation="EDSSSEELLSDNTLEKDENSPDNGFEVGEYNTHEAYNSENQYFT
                     REDYTYNYDLLNAI"
     gene            complement(574..950)
                     /gene="rnpB"
                     /locus_tag="ACHJ1G_003086"
     ncRNA           complement(574..950)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ1G_003086"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(983..2128)
                     /gene="garK"
                     /locus_tag="ACHJ1G_003087"
     CDS             complement(983..2128)
                     /gene="garK"
                     /locus_tag="ACHJ1G_003087"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4764894.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGVAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2225..3115)
                     /gene="garR"
                     /locus_tag="ACHJ1G_003088"
     CDS             complement(2225..3115)
                     /gene="garR"
                     /locus_tag="ACHJ1G_003088"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4764895.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3145..3915)
                     /gene="garL"
                     /locus_tag="ACHJ1G_003089"
     CDS             complement(3145..3915)
                     /gene="garL"
                     /locus_tag="ACHJ1G_003089"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4764896.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3931..5265)
                     /gene="garP"
                     /locus_tag="ACHJ1G_003090"
     CDS             complement(3931..5265)
                     /gene="garP"
                     /locus_tag="ACHJ1G_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4764897.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5640..7211
                     /gene="garD"
                     /locus_tag="ACHJ1G_003091"
     CDS             5640..7211
                     /gene="garD"
                     /locus_tag="ACHJ1G_003091"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4764898.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7360..7695
                     /gene="prlF"
                     /locus_tag="ACHJ1G_003092"
     CDS             7360..7695
                     /gene="prlF"
                     /locus_tag="ACHJ1G_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4764899.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7695..8159
                     /gene="yhaV"
                     /locus_tag="ACHJ1G_003093"
     CDS             7695..8159
                     /gene="yhaV"
                     /locus_tag="ACHJ1G_003093"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4764900.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8214..9023)
                     /gene="agaR"
                     /locus_tag="ACHJ1G_003094"
     CDS             complement(8214..9023)
                     /gene="agaR"
                     /locus_tag="ACHJ1G_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4764901.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9272..10552
                     /gene="kbaZ"
                     /locus_tag="ACHJ1G_003095"
     CDS             9272..10552
                     /gene="kbaZ"
                     /locus_tag="ACHJ1G_003095"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4764902.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10575..11048
                     /gene="agaV"
                     /locus_tag="ACHJ1G_003096"
     CDS             10575..11048
                     /gene="agaV"
                     /locus_tag="ACHJ1G_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4764903.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11059..11850
                     /gene="agaW"
                     /locus_tag="ACHJ1G_003097"
     CDS             11059..11850
                     /gene="agaW"
                     /locus_tag="ACHJ1G_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4764904.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAF
                     LPIYFGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFV
                     AAAWLKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11840..12718
                     /gene="agaE"
                     /locus_tag="ACHJ1G_003098"
     CDS             11840..12718
                     /gene="agaE"
                     /locus_tag="ACHJ1G_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4764905.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12736..13170
                     /gene="agaF"
                     /locus_tag="ACHJ1G_003099"
     CDS             12736..13170
                     /gene="agaF"
                     /locus_tag="ACHJ1G_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4764906.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13167..14300
                     /gene="nagA"
                     /locus_tag="ACHJ1G_003100"
     CDS             13167..14300
                     /gene="nagA"
                     /locus_tag="ACHJ1G_003100"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4764907.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14651..15805
                     /gene="agaS"
                     /locus_tag="ACHJ1G_003101"
     CDS             14651..15805
                     /gene="agaS"
                     /locus_tag="ACHJ1G_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4764908.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGM
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15818..16678
                     /gene="kbaY"
                     /locus_tag="ACHJ1G_003102"
     CDS             15818..16678
                     /gene="kbaY"
                     /locus_tag="ACHJ1G_003102"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4764909.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16845..17321
                     /gene="agaB"
                     /locus_tag="ACHJ1G_003103"
     CDS             16845..17321
                     /gene="agaB"
                     /locus_tag="ACHJ1G_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4764910.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17360..18163
                     /gene="agaC"
                     /locus_tag="ACHJ1G_003104"
     CDS             17360..18163
                     /gene="agaC"
                     /locus_tag="ACHJ1G_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4764911.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18153..18944
                     /gene="agaD"
                     /locus_tag="ACHJ1G_003105"
     CDS             18153..18944
                     /gene="agaD"
                     /locus_tag="ACHJ1G_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4764912.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18945..>19653
                     /gene="agaI"
                     /locus_tag="ACHJ1G_003106"
     CDS             18945..>19653
                     /gene="agaI"
                     /locus_tag="ACHJ1G_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO4764913.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPA"
BASE COUNT         4772 a         5129 c         4999 g         4753 t
ORIGIN      
        1 tagaagactc tagctcagaa gagttacttt cagataacac cctcgagaaa gacgaaaatt
       61 ctccggacaa tggttttgag gtcggggaat ataacacaca tgaagcatat aactcagaga
      121 atcaatattt taccagagag gactatacgt ataattacga ccttttaaat gcaatataga
      181 cttaataact tcttacaaag tctattatta ataccgtaga tatttattca tcttaccctg
      241 tgcaccaaaa gcaaaaactc actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc
      301 ctgatgcgct acgcttatca ggcctacgcc atctctgcaa tatattgaat ttgcgtgctt
      361 ttgtaggcag gataaggcgt tcacgccgca tccggcatga ataaagcgca ctcaacaatc
      421 tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt
      481 cttttgggta tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
      541 cgccggttta cgctggcggg ttttcgttaa atcaggtgaa actgaccgat aagccgggtt
      601 ctgtcgtgga cagtcattca tctaggccag caatcgctca ctggctcaag cagcctaccc
      661 gggttcagta cgggccgtac cttatgaacc cctatttggc cttgctccgg gtggagttta
      721 ccgtgccacg gactgttacc agccgcgcgg tgcgctctta ccgcaccctt tcacccttac
      781 ctgatcccgc ttgcgcgggc catcggcggt ttgctctctg ttgcactggt cgtgggtttc
      841 ccccccaggc gttacctggc accctgccct atggagcccg gactttcctc ccctccgccc
      901 gtctcccccg aagaggacga cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt
      961 atagagggtt tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg
     1021 cgatattacg tgaagcacgg cagatattgt cataagcccc gcggaatgct tcgtccaacg
     1081 taccaatgct ggtcagtacg ctgaagacag catcaatgcc atgctgatgt acaacgccaa
     1141 catcatcggt caggctaccc gcaatgccaa tcaccggttt atggtacttc ttcgccacgt
     1201 ttgcgacgcc aatcggtacc ttgccgtgaa tactctggct gtcaatacgc ccttcaccgg
     1261 tgatcaccag cgtacaatcg tgaatatgct cctccagatt cagcgccgta gtgacgattt
     1321 caataccact tttcagttcc gcaccaagaa acgccattag cgccgcgccc ataccacccg
     1381 caactcctgc gccggggaca tctttcacat caacatgcag cgctttttta atgacctcgg
     1441 catagtgaga gaggttattg tccagctcaa caatcatcgc ttcactggct cccttttgtg
     1501 ggccaaagat gcgcgatgcg ccgttatcgc ccaccagcgg attggtgaca tcacaagcga
     1561 cgcgaatgac gcaatctttt aagcgcggat cgaggccgga aatatcaata tcattcagag
     1621 tattaagact accgccgcca aaaccaattt cattgccgtt ggcgtcgcat aatttcgccc
     1681 ccagcgcctg taccatgcct gcgccgccat catttgtagc gctaccgcca atgccgataa
     1741 taatgtttgt cgcgccgctc tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag
     1801 tggtcacgag tggatcgcgt ttttccgcag gtaccagctc cagcccactg gccgccgcca
     1861 tttcaataaa cgcggttttg ccatcgccgg agatccccca actggcattc actttctcgc
     1921 ccagcggccc tgtaacccag gcgtgacgtt cagaaccctg ggtggctgca atcatcgctt
     1981 ccaccgttcc ttcgccaccg tcggcaaccg gaatagaaac gtactgtgca tcaggaaaaa
     2041 tttcccgaaa tcctttttct atcgcctgcg caacctcgct ggcagataaa ctttctttat
     2101 aagagtctgg ggcgattacg attttcatac ctatgcctgt taccacatga cgccggaggg
     2161 cgtttctctt attcggcctg gattccaggc ccggattgca atacgccatc cgggcacgac
     2221 gtcattaacg agtaacttcg actttcgcca gtttttcgta gtagcacgcc agggcgctat
     2281 gatccgccgt tcctaaacca tctgctcgca gtgcctgcat catctccata accgcagctg
     2341 tgagcggcag ttgcgcgccg acgccgtggg aagtatccag cgcatttgcc agatccttaa
     2401 tatgcagatc aatacggaag cccggcttga agttgcggtc catcaccatc ggcgctttgg
     2461 catccagcac ggtactgccc gccagtccac cgcgaattgc ctgatacacc aggtccgggt
     2521 taacgcccgc tttggttgcc agcgttagcg cttctgacat cgcggcaata ttcagcgcca
     2581 caatgacctg atttgccagt ttggtgacgt tacctgcacc gatttccccg gtatgcacca
     2641 cggaacccgc catcgctttc atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc
     2701 ccaccatcac tgacagcgta ccgtcgatgg ctttcggttc accgccgctc accggagcat
     2761 ccagcatatc aatgcctttc gctttcagcg cttcgctgat ttcacggctt gccagcggtg
     2821 cgatagaact catatcgatc aataccgtac ctggcttcgc gccttcaata atgccattct
     2881 cacccagcgc cacctctttc acatgagggg agtttggcag catggttatg atgacgtcgc
     2941 actgttcagc gatcgcttta gccgtagacg ctgtttctgc acctgcagca atcacgtcag
     3001 caatagcttc tgggttacgg tcagcaacca ccagcgagta acctgctttc agaaggtttt
     3061 tactcattgg tttacccata atccccaggc caataaaacc aactttcata gtcatatcaa
     3121 tcatctctct tgttgcggtg gtggttattt tttaaaggta tcagccagtt tctgagtggc
     3181 agagcggaag acgccaagat cgctgccgac agccacaaac gtcgcgcccc attccagata
     3241 acgacgcgca tcggcttcga ccggcgcgag aataccgctg ggtttgccgt gcgcactggc
     3301 acggttaaaa atgtgctgaa ttgctttttg tacatccggg tgcgaggcat tgccgagatg
     3361 gcctaatgcc gcagccagat cgctggggcc gacgaagata ccgtctacac cttcggtagc
     3421 ggcaatggca tcgacgttat ctacgccctg ctgactttct atctggacca gaatggtgat
     3481 gttcttgttc gactgagcga aataatccgc cacggtgcca aacatattgg cgcggtgaga
     3541 aacggagacg ccgcgaatgc cttccggtgg gtaacgggtt gatgccaccg cctgctctgc
     3601 ttcctctttt gtttctacaa aaggaatcag gaagttatag aaaccgatat ccagaagacg
     3661 cttaataatt accggctcgt tggtcggcac tcgcactact ggcgcgctgg cgctgccttt
     3721 cagggccatt aactgcggaa taaacgtgga gatatcgttt ggcgcatgtt cgccatccag
     3781 caccagccag tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc
     3841 tgaccagcaa ccaatttgta cctgtttcgc agccagtgcg gctttgaatt tattcgggaa
     3901 aacatcgtta ttcatcgctt atacctttgc ttatttctgc aattccatac gtttaatgtc
     3961 gccaactacg aagaggtagc agaccatcgc catcagcgct gaacatccca cgaaaaccag
     4021 tgctgcattg aaggagtgca gttcacttac caggtagcca atcaccagtg gagtgacaat
     4081 ggaggcaaca ttgccaaaga cgttaaagac gccgccacag aggccaacaa tctctttcgg
     4141 cgcggtgtca gaaatcaccg gccagcccag agcaccaaat cctttgccaa aaaaagccag
     4201 cgccatcagc atgaccacca gcgtggtgtt gttggtgtag ttacataaga tgatggtgga
     4261 agccagcaac attcccagca caatcggtag cttacgtgcc agggtcaggg ataaaccgcg
     4321 tttgatcaga taatccgaga agacacctcc cagcacgccg cccgcaaaac cacacagtgc
     4381 cggaatcgag gcgaccagac ccactttcag aatcgacatg cctttttcct gcaccagata
     4441 aatcgggaac caggtgagga agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa
     4501 tacgcccaac atcatgcggt tagagagcaa ttgcttgatg taatgcagtt tgggaccgct
     4561 tgctgccgcg ctgcccggct ttttgtggtc catatcgacc accgcgccat tttcagagat
     4621 aaacttcagc tcttccgcag acatacgtgg gtgatctgtc gggttatgaa tcaatttgat
     4681 ccacagcgcc gtcagcacaa aaccaatcac ccccataacg gtaaagacgt gctcccagcc
     4741 ccaggcgaaa gtcagccagc caagcagagg cgaaaagagc gccagcgaga aatattgcgc
     4801 cgagttaaag atggcggagg cagtaccacg ttctttcgtc gggaaccagg cggcgacaat
     4861 acgggcgttc gccgggaatg atggcgcttc cgagaagccg agcataaagc gcataaagaa
     4921 catggagatc cctgcccagg ccagcgggaa catatcaaca aagccttgca ggaaagtgaa
     4981 tagcgaccag aaaaagaggc tataggtata aacttttttc gagccaaact tatcaagcag
     5041 ccagccgccg gggatttgca tcagcaagta ggcccagcca aaagcagaaa agatgtaacc
     5101 catcgaaacc gcacttaact gcaactcttt tgccacttcg gtaccagcaa tagacagcgt
     5161 tgcacgatcg gcgtagttaa cggcggtaac aataaaaata atcagtaata ttaaatagcg
     5221 ggtatgcacg cctttctttt tttcgtcaac ggtgtccaga atcattttat ttacctcggg
     5281 tacttatgct gatttttatt attatgggga aggtgttatt tatgagtttc atttatgccg
     5341 taacggcaat gaactcggga attagtataa gcagcgcgag aataataatc attgtgcaaa
     5401 tgctaattta attaatgcta tttaaatatt attttgagca tatgcacata aggttgcgcg
     5461 ctaaagcaca gatttgcgct ttaccttgcc gggcggcact gcaatccctg aaatgattga
     5521 cattgatcac atttctgcgt ttaaactcct gacattctta tttcacccaa tgaagtcatt
     5581 tatttttaaa tgagaccagg tcctcatttt aataacccct ggctggagaa tatagtacaa
     5641 tggccaacat cgaaatcaga caagaaacgc caactgcgtt ttatataaaa gttcacgaca
     5701 cagataatgt ggcaattatt gttaatgata atggcctgaa agcaggaacg cgttttccgg
     5761 atggactgga attaattgaa catattcccc aggggcataa agtcgcattg ctggacattc
     5821 cggctaatgg tgaaattatt cgttatggtg aagtgattgg ttacgccgtg cgtgcaatcc
     5881 cacgcggaag ctggctcgac gaatcaatgg ttgtactacc ggaagcgccg ccgttacaca
     5941 cgctgccact ggcaaccaaa gtcccggccc ccttaccgcc gctggaagga tacacctttg
     6001 agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca
     6061 gcgtccactg tgtggcaggc gtggtggact atgtagtaaa aatcattgaa cgcgatctgc
     6121 taccgaaata cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac ggctgtggcg
     6181 tggcgattaa cgcaccggcg gcagttgtgc ctattcgtac catccacaat atttcgctga
     6241 accctaactt tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag ttgcagcctg
     6301 agcgcctgct ggtcggcacg gatgatgtgc aagctattcc agtagaaagc gccagcattg
     6361 tcagtttgca ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat attttgcagg
     6421 tcgccgaacg ccatctacaa aaactgaatc aacgtcagcg agaaacctgt ccggcttcag
     6481 aactggttgt cggcatgcag tgcggtggca gcgatgcctt ttctggcgta acggcaaacc
     6541 cggcggttgg ctatgcgtct gatctactgg tgcgctgcgg cgcaacggtg atgttctcag
     6601 aagtcacgga agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg aacgaagagg
     6661 tcggcaaacg gctgctggag gagatggagt ggtacgataa ctatctcaat atgggaaaaa
     6721 ccgaccgcag cgccaaccct tcgccgggca acaagaaagg cggtctggca aacgtggtag
     6781 agaaggcact cggctccatt gctaaatcgg gtaaaagcgc aattgttgaa gtgctgtcgc
     6841 ccggtcaacg cccgactaaa cgcggattaa tttacgccgc gacgccagcc agcgattttg
     6901 tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg accggtcgtg
     6961 gtacgccgta cggcctgatg gcggtacccg tcattaaaat ggcgacccgc accgagctgg
     7021 cgaaccgctg gtttgattta atggatatta acgcgggcac tatcgccacc ggcgaagaaa
     7081 ccattgaaga ggtgggctgg aagttgttcc actttattct cgacatcgcc agcgggaaga
     7141 agaaaacctt ctcggatcaa tgggggctgc ataaccagct ggcagtgttt aacccggcac
     7201 cggtgacctg atttcttttg gaacgtccct cgcaaaacat ggccttagtg ccatgttttt
     7261 attgtttaaa gcccccacgt ccattaataa tgcatttgca ttaccttagt tcaagcttat
     7321 aatttgagca gaaaacaggc tgtaaaagga cagtgaatca tgcccgctaa tgctcgctct
     7381 cacgctgtac tgaccactga atcaaaggtc acgatacgcg gacaaacaac tatccccgca
     7441 ccagtgcgtg aggccttaaa actgaagcca ggccaggaca gcattcatta cgaaattctg
     7501 cctggtgggc aagtctttat gtgccgactg ggagatgaac aggaggatca tactatgaat
     7561 gcatttttgc gttttctgga tgcagatatc cagaacaacc cgcaaaaaac tcgtccattc
     7621 aacattcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga tgatgagatt
     7681 ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta tatgctcatc
     7741 cctgttttca ggaaacctac gacgctttag ttgccgaagt cgagacatta aagggaaaag
     7801 atcctgaaaa ttatcagaga aaagccgcca caaagttatt ggcggtagtc cataaagtga
     7861 ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc aagtcgttag
     7921 gctctgggaa aaataaagac tggtcacggg taaaatttgg tgctggtcgt tatcgtctct
     7981 tctttcgtta tagtgaaaaa gagaaagtca tcattctggg atggatgaac gatgaaaaca
     8041 ctctgcgcac ctacggtaaa aaaacagatg cctataccgt attcagcaaa atgttaaaaa
     8101 gaggacatcc tcctgccgac tgggaaaccc tcacccgaga aacagaagaa acccattgat
     8161 ggtgttcaca atgcccgccg aacgtattcg tgttgaccag cgggcaccct ttctcactcc
     8221 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     8281 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     8341 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccacaacttc gcacatccga
     8401 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     8461 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     8521 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     8581 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     8641 tgcttacgca tcagacgagc aatctcaaaa gtggtagtcc cggaatcgag gatcacccga
     8701 tgaccaggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     8761 agcgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     8821 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag atcattgcgg
     8881 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     8941 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgccagtcac tcgcttctca
     9001 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     9061 ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat tgacgcaagc
     9121 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     9181 tctttcgttt tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa acgaaagatt
     9241 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     9301 gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt tccgcacatc cgctggtgct
     9361 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     9421 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     9481 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgttgattc tgggtggtga
     9541 ccatctgggg ccaaaccgct ggcaaaacct gccggccgct caggcaatgg ccaatgccga
     9601 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     9661 gtcctgtcat gacgatccga ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     9721 tctggcgaaa gtggcggaag aaacctgtct tgaacacttt ggcgaagccg atctggagta
     9781 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     9841 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     9901 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     9961 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
    10021 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aaacgccgca
    10081 atcgctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc cagcgctgac
    10141 cttcgccctg cgtgaagctc tgttctctct ggcggcgatt gaagaagaac tggtgccagc
    10201 gaaagcctgt tctggtctgc gtcaggtgct ggaagacgtg atgctcgacc gcccggaata
    10261 ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg gttatagcta
    10321 ctcggatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt tcgctcatct
    10381 ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt atctgccgct
    10441 gcaatacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac tcattatcaa
    10501 ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat aagcaaaaca
    10561 aagaggaaca cgctatgcca aatattgttt taagccggat tgatgaacgc ttgattcacg
    10621 gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg gtagccaacg
    10681 atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg gcagaaggga
    10741 tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc gccgccgatc
    10801 gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg gtgaaaggtg
    10861 gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc aaacaacaaa
    10921 tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac ctgaaagccg
    10981 ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtagac ctctttaaat
    11041 tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt gggcattatc
    11101 gcctttatcg ctggcctgga tatgtttaac ggcctaacac atatgcaccg cccggtggtc
    11161 ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt aaccggcggt
    11221 acgctggaac tggtgtggat ggggctggcc ccgctggcgg gcgcacagcc gcctaacgtg
    11281 attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa acccgatgtc
    11341 gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac cttcctgttc
    11401 tcggtgatgt ctggcgtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac
    11461 gccgatactc gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc
    11521 tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt
    11581 gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca
    11641 atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc
    11701 ctgggctttg ttgccgcagc ctggctcaag ttaccggtgc tggctattgc tgccgctgcg
    11761 ctggcgatgg cgctgatcga cctgctgcgt aaatctcctg aaccgactca acctgcggca
    11821 cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa
    11881 cgaagaaaca ttgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc
    11941 ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc
    12001 ttttaactac gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa
    12061 aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt
    12121 caatacccat ccgtttctgg tgacatttgt tatcggcatt atccttgcga tggagcgttc
    12181 taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg
    12241 gattggcgat gcaatgttct ggctaacgct actgccgatt tgtggcggga ttggagccag
    12301 tctggcattg caaggctcca ttcttggcgc agtcgtcttt attctgctgt tcaacgtggt
    12361 acacctgggg ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat
    12421 tccactcatt aaagcgaata ccaaaaaagt cggccatgct gcgtctatcg ttgggatgac
    12481 ggtaatcggt gcgctggtgg caacctatgt tcgtttaagc accacgctgg aaatcacagc
    12541 gggcgacgca gtggttaagt tacaggctga tgttatcgac aaactgatgc ctgccttctt
    12601 accgttggtc tacaccctga ccatgttttg gttggtacgc cgcggctgga gtccgctgcg
    12661 tctgattgca gttaccgtgg ttctcggcat cgtcggtaaa ttctgccatt tcctttaaat
    12721 acaatgaggt attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagcggc
    12781 atggaaaaag caatgaagca aattctcggt gagcaatcgc agtttatcgc catcgatttt
    12841 ccggaaacct caagcaccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg
    12901 gattgtgaag acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta
    12961 gcttcaacgc tggcgctgca aaaaccgggc tgtgaagtga tcaccggcac caatttgcaa
    13021 ctgctactgg agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag
    13081 gcgctggagt gtggacatcg tgggctgacc agtctggtgg acgagttagg ccgctgccat
    13141 gaagaatgtc cggtcgagga agggatatga cacacgttct gcgcgccaga aggctgctga
    13201 ctgaagaggg atggctcgat gaccatcagt tgcgtattgc tgacggtgtc atcgcagcaa
    13261 tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga actgctctgc cccgcttaca
    13321 tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac
    13381 tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggttg ccgactaccg
    13441 taaccgcgcc gcttaacacc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac
    13501 gcggcggacc tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc
    13561 agaataaagg cgcgcatccg ccggaattgt ttcgcgagct tgaaattgcc gagctggatc
    13621 aattgattgc cgtttctcag cacaccttac gcgtggtagc gctggcaccg gaaaaagagg
    13681 gggcattgca ggccatccgc catcttaaac agcaaaatgt acgagtgatg ctggggcata
    13741 gcgcggcgac ctggcaacaa actcgcgccg cgtttgatgc tggtgccgac ggcctggtgc
    13801 attgctataa cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat
    13861 taacggacaa gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg catccggcgg
    13921 cgatgtcgct gtgttgttgc tgtgcaaaag agagaatcgt gctgatcacc gacgcgatgc
    13981 aggcagccgg gatgccggat ggtcgctata cgttatgtgg cgaagaagtg cagatgcacg
    14041 gtggcgttgt ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct gttgatgcgg
    14101 cagtgcgcaa catggtcgag ttgacgggcg taacgcctgc ggaagccatt catatggcat
    14161 cgctgcatcc ggcgcgaatg ctgggtgttg atggtgttct gggatcgctt aaaccgggca
    14221 aacgcgccag catcgttgcg ctggatagcg ggctgcatgt gcaacaaatc tggattcaga
    14281 gtcaattagc ttcgttttga tattttgctc ctttattggg ccttcacttc ccccgtaagg
    14341 cctttctttt tctttcgttt tgatctgtgc agcggtgtcg gatgcgacgc taacgcgtct
    14401 tatgcgacct acggtacgcc gcatccagca atcggtgaac atagcgacgt cattacgcaa
    14461 ttctttaatc tccccttgtc ttttctttca atgatcacag atttcgtttt atttcctttc
    14521 tctccattga actttcggtt tcttttctat agattttaat caacgaaaga catcaccaag
    14581 tgaaatgaaa cgaaaggcaa gtgaaagcga caacgcccga cgtcaagttc atcagactaa
    14641 ggattgagtt atgccagaaa attacacccc tgctgccgcc gcaaccggta catggactga
    14701 agaagagatc cgccatcagc ctcgcgcatg gatccgttca ctcaccaaca tcgacgcgct
    14761 acgttctgcg ctcaataact tccttgaacc gttactgcgc aaagagaatc tgcggatcat
    14821 cctgaccgga gccggaacct cggcatttat cggtgacatc atcgcgccgt ggctcgccag
    14881 ccataccggt aaaaacttca gcgccgtacc gaccaccgat ctggtcacca atccgatgga
    14941 ctacctgaac ccagctcatc cgctgctgtt gatctccttc ggtcgatccg gcaacagccc
    15001 ggaaagcgtc gctgccgtgg aactggcaaa tcaatttgta ccagaatgct atcacctgcc
    15061 gatcacctgc aacgaagcgg gcgctcttta ccaaaacgcg atcaacagcg ataacgcgtt
    15121 tgccctgctg atgcccgcag aaacgcacga tcgcggcttt gcgatgacca gcagcattac
    15181 caccatgatg gccagctgcc tcgcggtttt cgcacctgag acgatcaaca gccaaacctt
    15241 ccgcgacgtg gcggatcgtt gccaggcgat cctgacctca ctgggcgatt tcagcgaagg
    15301 tatgtttggt tacgcaccgt ggaaacggat cgtttatctc ggcagcggtg gcttacaggg
    15361 cgcagcacgc gagtcggcgc tgaaagtgct ggaactgact gcgggtaaac tggcggcctt
    15421 ctatgattcc ccgaccggat tccgtcatgg cccgaaatca ctggtcgata acgaaacgct
    15481 ggtggtggta tttgtctcaa gccaccctta cacccgtcag tatgatcttg atctgctggc
    15541 tgaactccgc cgtgacaacc aggcaatgcg cgtaatcgcc atcgccgcgg aaagcaccga
    15601 catcgtcgct gccggtccac atattatcct gccgccgtca cgtcacttta tcgacgttga
    15661 gcaggcattt tgcttcctga tgtacgccca gacgtttgca ctgatgcagt cgctgcacat
    15721 gggcaatacg ccggataccc catcagccag cggcaccgtt aaccgcgtgg tgcaaggcgt
    15781 aatcattcat ccgtggcagg cataagagga tcgcattatg agcattatct ccactaaata
    15841 tctgttacag gacgcccagg ccaatggcta cgcggtgcct gcttttaaca ttcataacgc
    15901 cgagacgatc caggcgatcc tcgaagtgtg cagtgaaatg cgatcgccgg tgatcctcgc
    15961 cggaacgccg gggaccttta aacatatcgc gctggaagag atctacgccc tgtgcagcgc
    16021 ctattccaca acctacaaca tgccactggc gctgcatctc gaccaccacg aatcgctgga
    16081 tgatattcgc cgtaaagtcc acgcaggtgt gcgcagtgca atgatcgacg gcagccactt
    16141 cccgtttgcc gagaacgtga agctggtgaa atcggttgtt gacttctgcc actcacagga
    16201 ttgcagcgtg gaagcagaac tgggccgcct gggcggtgtt gaagatgaca tgagcgttga
    16261 cgccgaaagt gcattcctga ccgatccaca agaagctaaa cgctttgtcg aactgactgg
    16321 cgtcgacagc ctggcggtag cgattggtac ggcgcacggc ttatacagca aaacgccgaa
    16381 gattgatttc cagcggctgg cggaaattcg tgaagtggtg gatgttcctc tggtgctaca
    16441 tggtgccagc gatgttccgg atgaatttgt ccgtcgcact attgaacttg gcgtcacaaa
    16501 agtgaacgtt gccacagaat taaaaatagc cttcgctggc gcggttaaag cctggtttgc
    16561 ggaaaatccg cagggtaatg atcctcgtta ttatatgcgc gtcggaatgg atgcgatgaa
    16621 agaagttgtc agaaataaaa ttaatgtctg tggttcagcg aatcgaattt cagcataatc
    16681 attagttttt tatgatttat cccaatgtac ttcccgatta atatcgggga gtgccttaat
    16741 ggaaaaggag atgactaaac cttaataaat acatcagtac aatatcgcaa caataatata
    16801 ttaaaaaaaa ttatattatt caactttatg gtgaggatta cacaatgagc agtccaaata
    16861 ttctcttaac ccgtattgat aaccgtctgg ttcacggtca ggttggcgtg acctggacat
    16921 ccaccatcgg tgcgaatctg ctggtagtcg tggatgatgt tgtcgctaac gatgatattc
    16981 aacagaaatt aatgggtatt accgcagaaa cctacggctt tggcattcgt ttctttacta
    17041 tcgaaaaaac cattaacgtc atcggcaaag ctgcaccgca tcagaagatc ttcctgattt
    17101 gccgtacgcc acaaacggta cgtaaattgg tagaaggtgg cattgacctg aaagatatca
    17161 acgtcggcaa tatgcatttc tcggaaggga aaaagcaaat cagcagtaaa gtttatgtcg
    17221 atgaccagga tctcgcggac ttacgtttta ttaaacaacg tggcgtgaat gttttcattc
    17281 aggacgtccc tggcgatcaa aaagaacaaa tccctgacta aatctaaaat cgccttaata
    17341 ttggtttgag gtaataaaaa tgcatgaaat aaccctactt cagggattat ccctggcggc
    17401 gttagttttc gttctgggga ttgatttttg gctggaagcc ttatttttat tccgcccgat
    17461 aatcgtttgt accctaactg gcgctattct cggtgatatt cagactggct taattaccgg
    17521 tggtctgaca gagttggctt tcgccggatt aacccctgca ggtggtgttc agccgcccaa
    17581 cccgattatg gcgggtctga tgaccaccgt cattgcatgg tctacgggcg ttgatgccaa
    17641 aacagcaatt ggtcttggcc tgccgtttag tttgttaatg cagtacgtca ttctgttctt
    17701 ctattccgct ttctcattat ttatgaccaa agccgataaa tgcgcgaaag aggcggatac
    17761 ggcagcgttt tcccggctta actggacaac gatgctcatc gtcgcttcag cgtatgcggt
    17821 gattgctttc ctctgtactt acctggcaca aggggcgatg caggcgctgg tgaaagcgat
    17881 gcccgcctgg ttgacccacg gctttgaagt ggctggcggt attctgcctg ccgttggttt
    17941 tggcttgctg ctgcgcgtaa tgttcaaagc gcaatatatc ccttacctga tcgccggttt
    18001 cctgtttgtt tgctacatcc aggtcagcaa cctgttgccg gttgccgtac tgggcgcagg
    18061 ctttgcggtg tatgagtttt tcaatgcgaa atcccggcag caagcgcaac cgcagcccgt
    18121 tgccagtaaa aatgaagaag aggactacag caatgggatc tgaaatcagt aaaaaagata
    18181 tcacccgtct gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac gaaaggatgc
    18241 aggcgggcgg ttttacctgg gcgatgttgc cgatcctgaa aaagatttat aaggacgaca
    18301 aaccgggctt aagcgcggcg atgaaagata acctcgaatt tattaatacc cacccgaatc
    18361 tggtcggatt cctgatgggg ttattaattt cgatggaaga aaaaggagaa aaccgcgaca
    18421 ccattaaagg cctcaaagtg gcactgtttg gcccaatcgc cgggattggc gatgcgattt
    18481 tctggtttac tttgttgccg attatggcgg gaatttgctc atcatttgcc agccagggaa
    18541 acctgctggg gccgattcta tttttcgccg tttacctgct tatctttttc ctgcgcgtcg
    18601 gctggaccca cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg cgagagaact
    18661 cgcagatgat tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc gggctgatcg
    18721 cttcgtatgt gcatattaac gtggtgacat cgtttgccat cgacagtacc cacagcgtcg
    18781 cgctgcaaca ggatttcttc gataaagtct tcccgaacat tttaccgatg gcctacaccc
    18841 tgctgatgta ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta atcggcgtga
    18901 cttttgtgct ctctattgtt tgttccgcat tcggcatttt gtaaatggaa cgaggcactg
    18961 cttctggcgg tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg
    19021 aaaactatac ggcgttaagt gaacgagcca gcgaatattt attggctgtg atccgtagca
    19081 aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc attactgacg tatcattatc
    19141 tggtagaaaa aatccaccag cagcaggttg atatcagcca gctcaccttc gtgaagctcg
    19201 acgaatgggt ggatctgcca ttaacgatgc ctggcacctg cgaaactttc ctacaacagc
    19261 atatcgtgca gctgctgggg ctgcgtgaag accagctcat cagctttcgc tccgaagaga
    19321 taaatgagac agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc ggtctggatt
    19381 tatgcgttct aggattgggg aaaaacggtc atcttgggct gaacgaaccg ggagaaagcc
    19441 tacaaccggc ctgccatatc agtcaacttg atgccagaac acagcaacat gagatgttaa
    19501 aaaccacggg tcgccccgtg actcgtggga tcaccttagg cctgaaggat attctcaatg
    19561 cccgcgaggt tttgttattg gtgactggcg aaggaaagca ggatgcgaca gaacgttttc
    19621 tcacggctaa agtctccacc gctatcccgg ctt
//
LOCUS       DAZFDP010000086        19424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFDP010000086 DAZFDP010000000
VERSION     DAZFDP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..834
                     /gene="csgD"
                     /locus_tag="ACHJ1G_003107"
     CDS             184..834
                     /gene="csgD"
                     /locus_tag="ACHJ1G_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4764914.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            839..1228
                     /gene="csgE"
                     /locus_tag="ACHJ1G_003108"
     CDS             839..1228
                     /gene="csgE"
                     /locus_tag="ACHJ1G_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4764915.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1253..1669
                     /gene="csgF"
                     /locus_tag="ACHJ1G_003109"
     CDS             1253..1669
                     /gene="csgF"
                     /locus_tag="ACHJ1G_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4764916.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1696..2529
                     /gene="csgG"
                     /locus_tag="ACHJ1G_003110"
     CDS             1696..2529
                     /gene="csgG"
                     /locus_tag="ACHJ1G_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4764917.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2593..3084)
                     /gene="ycdZ"
                     /locus_tag="ACHJ1G_003111"
     CDS             complement(2593..3084)
                     /gene="ycdZ"
                     /locus_tag="ACHJ1G_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4764918.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3186..3740)
                     /gene="ycdY"
                     /locus_tag="ACHJ1G_003112"
     CDS             complement(3186..3740)
                     /gene="ycdY"
                     /locus_tag="ACHJ1G_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4764919.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3764..4501)
                     /gene="ycdX"
                     /locus_tag="ACHJ1G_003113"
     CDS             complement(3764..4501)
                     /gene="ycdX"
                     /locus_tag="ACHJ1G_003113"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4764920.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4556..5494)
                     /gene="ghrA"
                     /locus_tag="ACHJ1G_003114"
     CDS             complement(4556..5494)
                     /gene="ghrA"
                     /locus_tag="ACHJ1G_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4764921.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5728..5815
                     /locus_tag="ACHJ1G_003115"
     tRNA            5728..5815
                     /locus_tag="ACHJ1G_003115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5762..5764,aa:Ser,seq:gga)
     gene            complement(5965..6807)
                     /locus_tag="ACHJ1G_003116"
     CDS             complement(5965..6807)
                     /locus_tag="ACHJ1G_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4764922.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6892..7089)
                     /locus_tag="ACHJ1G_003117"
     CDS             complement(6892..7089)
                     /locus_tag="ACHJ1G_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4764923.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7101..7592)
                     /locus_tag="ACHJ1G_003118"
     CDS             complement(7101..7592)
                     /locus_tag="ACHJ1G_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4764924.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7589..7963)
                     /locus_tag="ACHJ1G_003119"
     CDS             complement(7589..7963)
                     /locus_tag="ACHJ1G_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4764925.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8010..8384)
                     /locus_tag="ACHJ1G_003120"
     CDS             complement(8010..8384)
                     /locus_tag="ACHJ1G_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4764926.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8547..8768)
                     /locus_tag="ACHJ1G_003121"
     CDS             complement(8547..8768)
                     /locus_tag="ACHJ1G_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4764927.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8831..9307)
                     /locus_tag="ACHJ1G_003122"
     CDS             complement(8831..9307)
                     /locus_tag="ACHJ1G_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4764928.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9323..9808)
                     /locus_tag="ACHJ1G_003123"
     CDS             complement(9323..9808)
                     /locus_tag="ACHJ1G_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4764929.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9899..10717)
                     /locus_tag="ACHJ1G_003124"
     CDS             complement(9899..10717)
                     /locus_tag="ACHJ1G_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4764930.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11057..12127)
                     /locus_tag="ACHJ1G_003125"
     CDS             complement(11057..12127)
                     /locus_tag="ACHJ1G_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4764931.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(12124..13029)
                     /locus_tag="ACHJ1G_003126"
     CDS             complement(12124..13029)
                     /locus_tag="ACHJ1G_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4764932.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(13026..15411)
                     /locus_tag="ACHJ1G_003127"
                     /pseudo
     CDS             complement(13026..15411)
                     /locus_tag="ACHJ1G_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(15522..18371)
                     /gene="cah"
                     /locus_tag="ACHJ1G_003128"
     CDS             complement(15522..18371)
                     /gene="cah"
                     /locus_tag="ACHJ1G_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO4764933.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18743..>19424)
                     /locus_tag="ACHJ1G_003129"
     CDS             complement(18743..>19424)
                     /locus_tag="ACHJ1G_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4764934.1"
                     /translation="SDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEALY
                     RDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMA
                     GIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASP
                     LMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDTVVSVAR
                     AVWNWIFF"
BASE COUNT         4553 a         5214 c         4845 g         4812 t
ORIGIN      
        1 acttgattta agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat
       61 tttacccatt tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc
      121 tggtgcttct attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc
      181 atcatgttta atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaaccg
      241 tctttgcaag cgacagctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa
      301 ttacataata ttcaacgttc tctggacgat atatcttcag gctctattat tcttctggat
      361 atgatggaag cagataaaaa gcttatccat tattggcagg atactttgag caggaaaaac
      421 aacaatatca aaatattgtt gctaaatacg cctgaagatt acccgtaccg cgacattgaa
      481 aactggcctc atatcaacgg cgttttttat gccatggagg atcaagaacg tgttgtcaat
      541 gggttgcaag gcgtcctgcg cggcgaatgc tactttacgc aaaagcttgc cagttacctg
      601 attacacatt ctggtaacta tcgttataac agcacggaat cagccctcct tactcatcgg
      661 gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg
      721 ttgttcatca gcgaaaatac ggttaaaacg catctttata atcttttcaa gaagatagcc
      781 gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata acctcaggcg ataaagccat
      841 gaaacgttat ttacgctgga ttgtggcggc agaatttctg ttcgccgcag ggaatcttca
      901 cgccgttgag gtagaagtcc cgggattgtt aactgaccat actgtttcat ctattggcca
      961 tgatttttac cgagccttta gtgataaatg ggaaagtgac tatacgggta acttaacgat
     1021 taatgaaagg cccagtgcac gatggggaag ctggatcact ataacggtca atcaggacgt
     1081 tattttccag acttttttat ttccgttgaa aagagacttc gagaaaactg tcgtctttgc
     1141 actgattcaa actgaagaag cactaaatcg tcgccagata aatcaggcgt tattaagtac
     1201 gggcgatttg gcgcatgatg aattctaaat aaaaaattgt tcggaggctg caatgcgtgt
     1261 caaacatgca gtagttctac tcatgcttat ttcgccatta agttgggctg gaaccatgac
     1321 tttccagttc cgtaatccaa actttggtgg taacccaaat aatggcgctt ttttattaaa
     1381 tagcgctcag gcccaaaact cttataaaga tccgagctat aacgatgact ttggtattga
     1441 aacaccctca gcgttagata actttactca ggccatccag tcacaaattt taggtgggct
     1501 actgtcgaat attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga
     1561 tattgctaac cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac
     1621 ctcgaccatc caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc
     1681 ataaggaaaa taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga
     1741 tgcttaaccg ccccgcctaa agaagccgcc agaccgacat taatgcctcg agctcagagc
     1801 tacaaagatt tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac
     1861 attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt
     1921 ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg gtttataccg
     1981 ctggagcgcc agggcttaca aaacctgctt aacgagcgca agattattcg tgcggcacaa
     2041 gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac ggcggcaaat
     2101 attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg cggtgttggg
     2161 gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat tgccgtgaac
     2221 ctgcgcgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac cagtaagacg
     2281 atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacca gcgcttgctg
     2341 gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc
     2401 gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg ggatttgcag
     2461 aacaaagcag aacggcagaa cgacattctg gtgaaatacc gccatatgtc ggttccaccg
     2521 gaatcctgac agataaaaaa gccggaatta tcatccggca ttttactgtt taagcgaaag
     2581 cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg tctttgcact
     2641 acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc
     2701 cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat
     2761 aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc
     2821 gacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg cactaccgta
     2881 aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga tcgccagccc
     2941 tttcagccca ccttgcgggc aggcaaagta ggccgtacag cccaggaagc ccgcccagct
     3001 tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc ctgttgtgat
     3061 tgcaatagag agaagtatgt tcatgccgcg catgatagca gaaaacgcgg ccatgaatga
     3121 gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag tgacgatgat
     3181 cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg
     3241 gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac tttgccgagg
     3301 aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc ttcgctctca
     3361 tcttccgttg actgatcttc cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg
     3421 gctggcgtat ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc
     3481 gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc gccgataaac
     3541 aacgcattgt aatcggcaga gacttgcgcc atatcacagc ttttctgtaa acgcgtcagt
     3601 aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat cagggtaaac
     3661 agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag cacacgacag
     3721 aggatagaaa actcgttcat taatatattt ccattactag taattaaagg tctgcaaatt
     3781 ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc ggcgaaacat
     3841 tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat tttaagacac tcttcaaatt
     3901 cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct ccagcatcac
     3961 gtaccgctgc ggctaccgca cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg
     4021 aagaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca acggctttca
     4081 catctatttc atatttggga tttccgggat ggcttattat atgcacattg ccgctggcga
     4141 tagtggcgat catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa accggctcat
     4201 gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcaa tcaatttcac
     4261 catcaacatt tttaatgtta gcttcgatgc cgcgcaggat ccctacccca tcaaccactc
     4321 gcggccagat acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc atatccgggc
     4381 catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca atgtaatcac
     4441 tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg acgggataca
     4501 taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg ctttattagt
     4561 agccgcgtgc gcggtcgact tgcccgcaga ccctctcccc tttttcgagc tgggcaatgg
     4621 tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta
     4681 tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca cgattaaaaa
     4741 catccaacat tgcgccttta actttgccgc tatccagcgc cgcgagcagg tcatcttcca
     4801 caacatgaac accacgcgcc aggttgagga gatacgcgcc atccggtaat ttttcgagta
     4861 attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta atcaataccc
     4921 gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc acgccaggcc
     4981 acgatttacg ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc agactctgag
     5041 caactttact gcccagtacg cctgcgccca aaatgccgat ggtaaaatct tcccgatgat
     5101 attcaggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg tcaaaacgtc
     5161 gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc ataccggtat
     5221 cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc tgtagcttgc
     5281 tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg cgccctgcca
     5341 gcatttcaac aggaggatgc cagactaaag cataatcagc agagtcatta tctccgcttt
     5401 tccacgctct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc caccattggg
     5461 tatcgaacgt tgggtgataa aagatgatat ccatactgac tcccgaaaag cgttgtgcga
     5521 aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa atccgtggtc
     5581 ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaag tttgaataaa
     5641 cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa attagcgccc
     5701 gttccagcaa gacaggaacg acaatttggt gaggtgtccg agtggctgaa ggagcacgcc
     5761 tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac cgccagatta
     5821 taagcgcatg ttattgttta acatgcgctt ttttattttc ccttccagac agtatgccat
     5881 cgggtatgcc atctgaaacc ggtttatatg ccccgcgatc atatcttatc ggacaatttc
     5941 cgatctttga gcatactggc aaagctatac tgccagcata tcaggatagt gttttgcgat
     6001 aatatcattc agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg cggagccctt
     6061 cttaaagtat ctgatgctga acatctcatc ttcatagctc tctttaccct gaacagactg
     6121 aatgtgatca tccagacgaa gggtgatgtt tccccggttg tcggggattg gtttaccgct
     6181 gaacaggtgc aacattcttt ccagatcggc gagtcggtca gcctgttgtc cggtgataag
     6241 atgaaatccc caccggtccc atctcaccag attgttgacg ataattttac tgccaaattt
     6301 acaggggcaa ttggttttgt aattccagtt cagccctctg aagacgttga tcacccctcg
     6361 ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc
     6421 gctgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac gggcctgcgt
     6481 atccattaat gacagcatcc cggaacgttg catcaggtca cgccagattt cgcgatcaag
     6541 attccgggta atggctttca ttgctgtttc gggtttctcc atcagccagc agccgcagcg
     6601 aaaatcctgt ttcatggccc agtccggggc agttttacct ccgatgcttt ccgtcagcac
     6661 cgagatttca gccagttgtc ggataagtgt gtcgatttgc aacagtgtac tctttcgtac
     6721 agccagaatg tggcgaatgc tggttgagca gataacgtcc gtatgcccgt tgaccacttc
     6781 cggacgggag attgtgatat ctgacataat cgatattcct ttttgcagat aaatgaaaac
     6841 gccagccctg tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt
     6901 ctggtgcaga agctgcagat gacgcagggt acggacatcc tcccgggcct gttcgataca
     6961 gggcaacagt gctgctgaat ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc
     7021 gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt ccgtggttaa
     7081 tgatttcatg ttgttcctgg ttaccagtcg aaagtgggta aacccggcag gcattcagcg
     7141 ctggcatcca gatgaatgag actgacgcca taacgccgga tgagtgtgac gaccagacga
     7201 cggaacgttt ttgacagccc caggcgttta agccgcagaa ccgggtatga ccacgcatcg
     7261 gtacgtaaca gatatccgct acctgtaaaa tgaatccatt ctgattcacc gaagtcactg
     7321 gtctggtgtg acagcgagta caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg
     7381 cagcgtatgc cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat
     7441 tcatcaacaa cacgaaggga gtagccgtta tcgcagacca tgttaatcag ttcagcgagc
     7501 tcaataccat cgacgtcaac gacaatgcgc cccatgttca gtgcctgtac gttaacgctg
     7561 tcggcttcca gtgtcagggc cagtttcatc atttcgcctc cggatactta cccaaggtaa
     7621 tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga
     7681 ggatatcaat actgtttatt aactgagagc ggggacaggt gctgaatccc ggctggtcgg
     7741 tacgcaccag cgcgtatttt tccacgagaa aattaactgc atcacacagt gaaatgcctg
     7801 cctcaatatg ctgctcaatc acacgttcat cggcaaacgg tgtgtcattc agtgtgaggc
     7861 cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg ggagacgggc
     7921 agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac
     7981 tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgagtgc tggtgtcgga
     8041 taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca ggtcagccct
     8101 tttgcattca gcgtgacggt atgctgatat cggggattca gttcaccgct ggtgagcatg
     8161 agttccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc atctgcgtcg
     8221 ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat gcaggcggtt accctcttgc
     8281 accagacgag cgccaaaaca gggcctcact gtgcagggaa gcccccacca ggggcggtcg
     8341 tgattgtcat cgggatgagt tatcccggag agtttgtctg acacgataaa atcctctctg
     8401 aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac cggtttcggt
     8461 gaagatgtat gcattgatga gaatggcctc atccacagcg gcgtctgagg tcatctggtc
     8521 gtactcgttt cccgggtgac ggaacatcag ttcagggtga cactcatcag ccggacatac
     8581 gggccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg aatatcctgc
     8641 acttcacgac cagtatacgc ttcagcactg ccgtgccagc gatacttacc ggtacagaac
     8701 ggaaaaagac gggatgtcgg atgttgttgg tgaatacgca tggcttcacc acgggtgata
     8761 attttcataa tgggatacct ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg
     8821 ttgtgaaggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg ccaccgacta
     8881 tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga cgttcggtga
     8941 taagccggtc tgccttactg ggtgtgactt caccggacgg gtgattgtgc gccagcacca
     9001 cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatga acttccgtac
     9061 ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat
     9121 acagcacccg gaactcttcc cgctccagtc ccgccatgtt cagaatcagc cattcccgtg
     9181 ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggtttgca
     9241 gggcccgcag aatgagactg cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct
     9301 gctgcattgt ccctgcctcc attcagtcaa tgatgcgcat aatggcgctg cattccggat
     9361 gctgcagggc gtaatcccgc agccggtaat agtggaccgt catggcataa cactccgtac
     9421 gacaggcatg atgactgtac gtcatcagac aggcagcaat accggcggct tccgggctca
     9481 tttcagcgcg gttgccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat
     9541 ccggttcggg tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac
     9601 cgcagtagtc ttcgcaaaga cggtccatcc agccgaagac acggggctcc agggtcaccc
     9661 actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca tcagggacgg
     9721 gagtggcaac cagctgaggt gggttattcc ctgatgcagt ggttacggaa acagaaggtg
     9781 tggtggaatt atgcgaaacg gttgtcatga ggttattcct tataaaaagt aaatgaatgg
     9841 aagaagcccc ggggcaggaa cagatgtgag tcaggactgc actttcagtg aaacggcatc
     9901 agcgcatgct ctccagcagc gtttctgcca tcacccacaa tgcgcgattg agcttaatgt
     9961 cggtatcaat gctgtgaatg gcccgggtat ggatacgttt tccttttgca ctgcgaccgg
    10021 aaatcccgcc tttcagcata ttctcctgga tggtctgata agcactccac aggtccttac
    10081 cgtaatcctc ccggcgtcgt ggtgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt
    10141 caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga
    10201 ccagcgactg catggcatca cgcttctcct ctatccggtc aaaaacgccc accacctcgt
    10261 aagccccttc aatgactctg tccaccacat ttccccggtg tggaacacgc aattctccca
    10321 gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg ggtaacatct
    10381 ggtagctgga tgaaccgtca tgggagttga gcagaataat ttcagggaca tgctgaccgg
    10441 ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg cggctctggt
    10501 cgcgcacgcg ggtctggcag gcaaagaacg gctgaaagcc ttcctgctgc agattttcca
    10561 ggacggtgat agtgggaatg tacgcatagt gttcactgcg agaggtatgt cggttttccc
    10621 caaaaatact cggtacatgg cgcatcagtt cttcgtgtgt cagcggacga tcacgacgta
    10681 tctggttcgc ataaccaaaa cgactggcta atctcatgat ttgctcctta tgaatgatta
    10741 agcgatataa atgaaaaagc cgcgtctccc ggagaagacg cggcctgatg ggtgaaatga
    10801 atgaggttta ttgtctgaga agcccttcac tggcgaacca cgtattaagc tgtattccgg
    10861 catcgccagt gcaactgacc ttcagcattg cggataaccc gccggaaata tgttcactgg
    10921 ttgtttttgt atcttccccc aaaattaaat aaattcatct taaccatcac ccagataata
    10981 tatatctgaa aatattttca ataacaccag acaggaaaaa caccaccaca ggcggtggcg
    11041 tttctttggg gcgtctttat ttaatccggg ccatcgcgtt tctcagtgcc aaatctgcct
    11101 cttccgttat atccaggctt ttctgcaata ccgcctctga ccgggtcagt gcctgacgtg
    11161 ctgccagcgg tttccggata tgctccatcg ccagcatcgt gtcttcatag ccctgctgac
    11221 gccaggcgtc ggtatgtgcc gatgtaaaac tgagcagacc gccgctgttg ccaccatcac
    11281 ctgcatattt cagccttttc cggggacgga tctcaaggat tgtggtgtcc ggaaaagcct
    11341 ggcgatccca taaagaaccg tcactcagat gcgtcacaat caccatattg catccggcat
    11401 cgaccagagg cgtcacaggg gtatttccct gcatatttcg ccatcctccc ataccaccat
    11461 caccgaacat tgtcccctga acctcacggg gacggaacag caggggcagc gcggctgacg
    11521 caagcagagc ctctttctgc tgtccgcggg gcaggctctg gatatgctga aaaacggcgt
    11581 ttttcgtggt tccgacaccc agttcagcac gaatgcagtc aataatatcc tgcatgcccc
    11641 cttctgtggg gtacagcgac acatacagcg gtagcccgtc tgccagagca tcagtatcaa
    11701 gataatgatc catcagcgct gtcaggggct catctgacaa caacggctga gccatcagat
    11761 tgtcaaaccc gttgattgtc gatatatggc gaagaagcgt cgtaagcagt gctcctgcac
    11821 gtccggggat ctggcagagg ttcattgcct ggaacaattt tttcagcaat gaaaagtaaa
    11881 ccaatctgtt taccgacagc acctgattat tccccagatg ctcccagagc gcctccaggc
    11941 gtacggcagc ttctgacaga tcgggagagg ccgcgataat ggcaccattg aatgcgccaa
    12001 tgctggtccc tgaaaccatg ctgatctgtg taccacactc tgccagtgcc ttaaccactc
    12061 ccgcctgata agcgcccacc gccccgccac cggacagcac cagccccgtt ttcatttctg
    12121 tactcatgca gcccccttct gaaatatttc atcgaccata ctttcagaga ggcgttctgc
    12181 agagcagagg cgaggcatgc gaaccggtaa gtgttctgca gcctgcgtgg ttgaccagat
    12241 caccggatgc tgctcttcac tgcaccagtc tcccagccag ttaatcgcgt catgccactc
    12301 gttatctgcc tgctcccggc cctgaccacc aagccagtcc agagtgttca gacgcacggt
    12361 tgcactaccg ccactctcct gtgccatctg cgttaatgct ttatgtctga gaatatccag
    12421 tagctggtta acctggcctg tttcgccctg acgtatcaca tcaccaaacg cgccccagcg
    12481 aagctcttca agtgccacat aacaacgtcc ggcaggggaa aatgacgcat atcgcccggc
    12541 gctccaccat gagaatatct gctcaagatg taactgcgcc cgctgaacct ggtcaacacg
    12601 gtggagtttt tcttcaagat gattcagttt atggtgaacc tgatcggcca gggtgagtaa
    12661 ctgctcccgc gtatctgcgg aataatgcgc cagcctggct gtatcactga ccagtgaact
    12721 gaccctgtcg tttacccggg taatcgcgta gtttgtggtg gcaagtgacg ttgtcatctc
    12781 cgtcagccag cgcagggacg cctcaacgcc ctgtgcaagc gacgcattga tggcattctg
    12841 ccgtgcagcg ccttcgccgg tcagcccctc ttttaaacgc cggaagaacg tgcgctctcc
    12901 ggccgtgcgg atatgctcct gtgcaacatc gattccgttt gcgaaatcaa ccacaaattt
    12961 ttcaggaaga caggtgagta cctgacatac ccggttatca gcaatttgtt gaataaatgg
    13021 tgaagtcaca gcattgtctc cagctcttcc ctcagcgcct tactgtcagt aatgagctca
    13081 ggaacccttt catttaatgc ctgaagtcgc ccggtaagcc gttcctgcgc ctgctgatcg
    13141 cgtttatgat cttcagagct ctgaatcagc gtgttacgca gttgttcaac tttctccctg
    13201 aatgcgcaga aaaaggcatc gatttcctgg cggacgggct ggttaatatc ctgctcaatg
    13261 gatgcctgca gctccccgaa atatgcccgg gtcagtgaca taacttcctc tctggccttg
    13321 ttgatattga tcacgctgcg actcacatcc tcgtcatagt tttcccagcc caggtcactg
    13381 gtgccaaacc atcgacaaac ggttccccat acgccactct gccgacggcg acgggtgacg
    13441 gtctccgttc tttcctccat aagcgcactc agatttattt ttaccgcgag ccgggtctgt
    13501 aactggctga ctgccggaaa actgattttt acggtaaagc cctcatcctc caggcgggca
    13561 ttgatgcgac cggcaatgtt ttctgtggcg tacagggccg tgccacgaaa acctgtttca
    13621 atcccgctga cgattttctc cagctcaggc cggaactgtg cttcagcctc acggtgcagg
    13681 ctggtcacgg tcgattcgat ttgcgtcatc agttcaaggg ctgccctgcg atcactgaat
    13741 tttatttcgg ggctgtccgg atcaaagtcc cgcatcctgt catgctgatt tccggttccg
    13801 aaaatatcgt gaaagaaagc gaatgcgttt ctctcccggg gctgcgctga attttcttcc
    13861 tccagcattt gctgttcttt gcgtttaccg cttctgaaat agtcgttcag ctcctcctcc
    13921 agtgaggcac agaccttcgt cagtaattcc ccggttttgg tattgatgtc ctccatggcc
    13981 attctgacat caccggtcac ctgattctga cattcctcaa tctcctggat atccgccagc
    14041 aggctggtaa tatgcgcctg tagtgactga atgctttgct gcagcccctg attgacgtaa
    14101 tgacagatat tcgctgacat tttctgcatt ctgcatcagt tttgcggcgg cagagtcaac
    14161 cgccagtgcc gccgctctgg aatgggagct ctgtatgact tccgttatca gttgatcgat
    14221 cagagatata ttccagagtt tttctgcctt tttatttacc ttagtgctgt ctttccagtc
    14281 gtcctcttcc accataggtc cgaaagcttc cctgataaaa tcatcgaccc aggcttcatc
    14341 aaccggaagc gcaccgtttt ttcgtaactc atgaagcgca cggttagcca ggtacgcctg
    14401 acgtgatgag ccgggataaa cgcgggaggc gggcagcaca tcgctgttca gcattgctgg
    14461 caccttctgg cgtacggcat ccgcaccatc accattgcga tctttttcat cgaatttatt
    14521 gaccagcaca aacagacgac cggctgagac gtcagcaatg gcattaagct ctttacgaac
    14581 ctcctcatcg gctttcgagt tcatctgggt gtaatccatc accgccagca ccgcagaggc
    14641 tttctgtagt tgatcccgca tcatcatttc catctgtggt tgtccggctt cattagggcc
    14701 gggggtatcc agcagtgtga gtgttccctg acatgcatcc attcccacca ggtggctgaa
    14761 cgccacgtcg atcaccggta gtttctgcac ttctgcgtat tcgtcaaagg gaaattcagt
    14821 ccccattgcc gcagcaagac gaaccaggtc atttaacgat gccagtccgg taaagatttc
    14881 gtcttccccg tggtattcat ttttaagcca gacatcatcc gccagaatat ccagtagttc
    14941 gcgggtatcc ccggtttgct gcagggactg tgcgacttgc tgccctgccg gggtggcgag
    15001 tttttcctgc agtgtgatta ataaattgcg gacgggctga atatgttcca gatggaggac
    15061 cggttccgtt tttccgggaa cgtggcggat caatgtcggt accgaggtca tggggcggtt
    15121 acggttgggc agaatttcct gccccacaat ggcgttgatg gtggttgatt tgcccgcctt
    15181 catcgtgccg accacagcca gcaccatctc cctgcgggcg gttttgattt gttcgccctc
    15241 cagctcctca attcttttca gcgctttccg tttatcaaaa agcagcccgt tttcgttctt
    15301 actgtcagat aacacatccg gctcatcaac catttgccgg agcagattga tattcagttg
    15361 caaaagccgg tcggcttcat cacaaagcag ggcgatgttc ttttcgtgca tcattcacct
    15421 ttataaattc aataattcag aactgcggtg aacagcaatg agcgtccttt cccattactg
    15481 catgaatttt cctgaaatcg tatttccggg ataccgtata atcagaaagt catattcagc
    15541 gtagcctgac cgttatagcc ttcagcgctg ctgccgctga cgctgtgggc ataaccggcc
    15601 tgaacgccca gggtgatatt ttcccggata cgggcttcca gtccggcctg caggtccagt
    15661 gacgtgccat tccgggacgg tgagaacgtc atgttactgc cggctgcggc tgtccccatg
    15721 ctcatgtcac cccgggagct gaaggtgcgg ataacagaag gctgtaccca ccagttcacc
    15781 ggcagttcac tcacactgtg ttttgcactg tcgcgcaggg tgtcacggga tgaggtgcct
    15841 tcaccaaagg tcatatcgtt gtggctgccc agacggaaac cggcacgcac atgttgtgca
    15901 ctgccatgcc cgaacttcac ataaccggcg ttatcctggc cgtcatccag ggagagtccc
    15961 tgccaggtgt actgcagttg tggctccagc atcagattgt cagtgatact gaagggcaga
    16021 ccggtttcca gtgagcccag ccagccccag ccccgggcgc ggaagtcgtt attgtccgat
    16081 gacgctttca tgctgtgacg ggttccctgg gccacaatgt cagcccacag gccggaggat
    16141 gtgtgtacca gattcaggta tccgcccaga ctgccggcat catcccggac cgtgccggcg
    16201 cgggaaccgt catcatcctt aacatcaacg gaagaatggc ctgcagcacc atacacccct
    16261 gtcgtcagag acataccggc aacctctgtt ctgagcaggt caccctccag acggacgaag
    16321 ccatagctgc cgctgctttc cggcgtggct ccacgggcaa taccgccgtt gttatcgtga
    16381 ccgagatgac cgccctgaat gctgagacgg acgctgttat tttcaccgtt tacaccggtc
    16441 tgatggctgc gggagcctgc cagaatccgg tcatagtcca ttgcctgtgt caacatggat
    16501 gtatacaggg ggacttcagc acgataagca ttttcactgc gcaggtacca gtcttcatcg
    16561 ctgtcacggt tcagggtgta gttaaaggcg ccggcctgaa gcgggcgact cagggcaaac
    16621 gcaccttctt ctgtggtggc gccattctgt gcatccacaa cccggatacc ctgtccggtg
    16681 gttgccaccc cgaggttgct gtttccgaca tttgtaaacg caagccaggt tttgccggtt
    16741 gcctgaccac cattaatcac cagctggtca gaggcattgc tgccatcaag gcgaacacgc
    16801 atattgatgg tgccgccctg gccggtgagg ttcgtggtgg tcagtttatg gaacgttacc
    16861 ggggagttac tttttgcaac agcacggctc agcgcggcat tcggtgtcgt ctgattatca
    16921 aagcgaatct cccctgcgtt aacaatatcg ccggtactga ccacatcacc attcagtacc
    16981 atactggcgc ctttactgag ctgtgttctg ccactcagac ttcccccggc agccagcgtc
    17041 agtgttccac gatgtccgac agtggtgttg ccagcctgtc ccccggcatt aaccgtaaag
    17101 ctgccgccat tttccagcag caggccgctg gcctgaccgg atgtgccatc aatactgaac
    17161 ttaccgctgg cattggtccc ctcaacagtg gcaccactgt ctgcaatcag ggcaccaccg
    17221 gatgttatgg tgacacttgt cgccttaccg ccggcagaca ctgccagggt accgccgtca
    17281 tccactaggg tattctgtgc tgaatggctc tccagtacat ccagacgacc gccggattcc
    17341 agaacaacac cgtcagcctt accgttttcc accgtgaaat tccccagacg gtttgtgccg
    17401 atgacagttg cagcagtact ggtaaccagt gcaccgcccg tgttctgggt gactgcagtg
    17461 gcttccccgc cggcatgtac cctcagttca ccgttctgat ttatggtggt gttaccggca
    17521 gcgccacctg ccttaacaac ctgccagccc ccctcgttaa ttaccgtgtt cagcgcaagt
    17581 ccgtccctgt gcacatactg gtaaccgcca ttcagtgtgg tattcagggc ccttccgtgt
    17641 accgactggt caccacctgc gtaaatgaga gtgtcacggg ctagcccgga aaataagata
    17701 atctgccgtc catttttgtt gatggtggtg gattctgctg ttccctggac cttctgaccc
    17761 gtatacgagt tgtcagaatc agggccgcct tctgcgccgg tgttgatgat ggttcctgtt
    17821 gccagcccgc cacttttaac gctctggtaa ccgtcgcggt tgataattgt accggtggca
    17881 accccgccct catgcaccca ctgctcgcct ctgttattca gtgtggtgtt caccgccagt
    17941 ccgttcaggc tctgtccccc gccgtcacga ataaccgtat cactggctgt tcccccctcc
    18001 agcacgacct gacgaccatt tgtggtgaca gtggtgtttc cggctatccc gccattctgt
    18061 atccattgcc caccggtgtt ttcttcactg tccggcccca gttccagccc ggtactgatg
    18121 gtcattccgt tggccgtacc gaagacaatc tggttgtcat gatttgtcag tgttccatcg
    18181 ttcacggttt ctcccgcctg tacaaccttg tcagcagcca gtgccgggac tgatgtgaca
    18241 gcagcaagag acagcgcaac cgccacaccg gcgcgttttc cccgtgagcg ggccagttcg
    18301 gaggccacca ccagggtgcc cgtaatgtga ttccatacca gcctgtagct ggtgttcaga
    18361 tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac ccacaaaagt
    18421 ggataccggc aggtacaaaa cagagttatc gggcacagcg atacgtcccg ggcgtaaacg
    18481 ctctccccct tcatcagaca gagaagtatc agtctgttgt gtgcaattcc gttctgcatg
    18541 atgaagagat tcagctgcct tttcgcaggc cattaaccac tataaacgat cgatataaac
    18601 gatcgatacc tgttttatcg atcgttttat tctattaaca gtagagcagt cacgcaagag
    18661 aaacaggagg ggcatgaggt gtttgtatca tgttttcagt tggttaacgc aatgtaatca
    18721 gacatttccc ctggcagact gttcagaaga aaatccagtt ccataccgcg cgggcaacag
    18781 agaccaccgt gtcacggacg gcacgcagaa ctgtgcgtgc aacagaggca acacagacgc
    18841 tctccaccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gcctgccccc
    18901 ggacagattc cgtgcacagc tcgtcctgca ggcgggtcat caggggactg gcggcatggt
    18961 cgggaagcgc tgtcatgagc gcactgacca gcgtatccag ttcccagccg gtgcgggccg
    19021 atacggcaac aaccggatgt acgggccgga acagccggaa taccgcatct gttttttcgc
    19081 gaatgttctg ttcctgtgcg ggagaaggct gaatacctgc catatcccat tcatggcagg
    19141 gctctgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc cggtgcagta
    19201 tgtgtcgcca gaaatactca tccacagaca gggcacggtc atcggcttta atcagccaca
    19261 gtaccaagtc cagttcaggc agaatgtcac ggtacagggc ttcatactct gcatccctgt
    19321 cccggctctc gcccaccccg ggcaggtcag tgataaccat gctgtgacca tggccactca
    19381 gacggaagcg ccgcacttcc cgggtgccgg cgtgaacatc actg
//
LOCUS       DAZFDP010000087        19387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFDP010000087 DAZFDP010000000
VERSION     DAZFDP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..143)
                     /locus_tag="ACHJ1G_003130"
     CDS             complement(<1..143)
                     /locus_tag="ACHJ1G_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764935.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLV"
     gene            252..746
                     /gene="napF"
                     /locus_tag="ACHJ1G_003131"
     CDS             252..746
                     /gene="napF"
                     /locus_tag="ACHJ1G_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4764936.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            736..999
                     /gene="napD"
                     /locus_tag="ACHJ1G_003132"
     CDS             736..999
                     /gene="napD"
                     /locus_tag="ACHJ1G_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4764937.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            996..3482
                     /gene="napA"
                     /locus_tag="ACHJ1G_003133"
     CDS             996..3482
                     /gene="napA"
                     /locus_tag="ACHJ1G_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO4764938.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3489..4184
                     /gene="napG"
                     /locus_tag="ACHJ1G_003134"
     CDS             3489..4184
                     /gene="napG"
                     /locus_tag="ACHJ1G_003134"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4764939.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4171..5034
                     /gene="napH"
                     /locus_tag="ACHJ1G_003135"
     CDS             4171..5034
                     /gene="napH"
                     /locus_tag="ACHJ1G_003135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4764940.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5031..5480
                     /gene="napB"
                     /locus_tag="ACHJ1G_003136"
     CDS             5031..5480
                     /gene="napB"
                     /locus_tag="ACHJ1G_003136"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4764941.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5490..6092
                     /gene="napC"
                     /locus_tag="ACHJ1G_003137"
     CDS             5490..6092
                     /gene="napC"
                     /locus_tag="ACHJ1G_003137"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4764942.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6105..6728
                     /gene="ccmA"
                     /locus_tag="ACHJ1G_003138"
     CDS             6105..6728
                     /gene="ccmA"
                     /locus_tag="ACHJ1G_003138"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4764943.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6725..7387
                     /gene="ccmB"
                     /locus_tag="ACHJ1G_003139"
     CDS             6725..7387
                     /gene="ccmB"
                     /locus_tag="ACHJ1G_003139"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4764944.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7429..8166
                     /gene="ccmC"
                     /locus_tag="ACHJ1G_003140"
     CDS             7429..8166
                     /gene="ccmC"
                     /locus_tag="ACHJ1G_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4764945.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8163..8372
                     /gene="ccmD"
                     /locus_tag="ACHJ1G_003141"
     CDS             8163..8372
                     /gene="ccmD"
                     /locus_tag="ACHJ1G_003141"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4764946.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8369..8848
                     /gene="ccmE"
                     /locus_tag="ACHJ1G_003142"
     CDS             8369..8848
                     /gene="ccmE"
                     /locus_tag="ACHJ1G_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4764947.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8845..10788
                     /gene="ccmF"
                     /locus_tag="ACHJ1G_003143"
     CDS             8845..10788
                     /gene="ccmF"
                     /locus_tag="ACHJ1G_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4764948.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10785..11342
                     /gene="dsbE"
                     /locus_tag="ACHJ1G_003144"
     CDS             10785..11342
                     /gene="dsbE"
                     /locus_tag="ACHJ1G_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4764949.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11339..12391
                     /gene="ccmH"
                     /locus_tag="ACHJ1G_003145"
     CDS             11339..12391
                     /gene="ccmH"
                     /locus_tag="ACHJ1G_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4764950.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12426..13073)
                     /gene="narP"
                     /locus_tag="ACHJ1G_003146"
     CDS             complement(12426..13073)
                     /gene="narP"
                     /locus_tag="ACHJ1G_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4764951.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13393..15984
                     /gene="yejO"
                     /locus_tag="ACHJ1G_003147"
                     /pseudo
     CDS             13393..15984
                     /gene="yejO"
                     /locus_tag="ACHJ1G_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16452..17375)
                     /locus_tag="ACHJ1G_003148"
     CDS             complement(16452..17375)
                     /locus_tag="ACHJ1G_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4764952.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(17365..17526)
                     /locus_tag="ACHJ1G_003149"
     CDS             complement(17365..17526)
                     /locus_tag="ACHJ1G_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764953.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            complement(17538..18026)
                     /locus_tag="ACHJ1G_003150"
     CDS             complement(17538..18026)
                     /locus_tag="ACHJ1G_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4764954.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(18154..18315)
                     /locus_tag="ACHJ1G_003151"
     CDS             complement(18154..18315)
                     /locus_tag="ACHJ1G_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO4764955.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(18319..18513)
                     /locus_tag="ACHJ1G_003152"
     CDS             complement(18319..18513)
                     /locus_tag="ACHJ1G_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764956.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            complement(18644..18889)
                     /locus_tag="ACHJ1G_003153"
     CDS             complement(18644..18889)
                     /locus_tag="ACHJ1G_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764957.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            complement(19091..>19387)
                     /locus_tag="ACHJ1G_003154"
     CDS             complement(19091..>19387)
                     /locus_tag="ACHJ1G_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4764958.1"
                     /translation="ELFTDADGELITSLVVVDKPRPPVELERIEEAGNKTENHAALWQ
                     CIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKLENDGVIVIDGDDVRPL"
BASE COUNT         4297 a         4758 c         5384 g         4948 t
ORIGIN      
        1 accagcagaa atatctctgg tgaattaatc gctatataaa tatgtttata accatttgaa
       61 atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc tgctacaggt
      121 tttaccccga tcggggtatg catctttgac acatccttta atatcttagc ggctataaaa
      181 atggcttatt aattatgcgg tttatttggt cgctctcaat tttcagagcg cgttaatgat
      241 ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt cgctggcgca
      301 aagccagtaa cggtatccgt ccgccctggt ccggtgatga atctcatttt ctgacccatt
      361 gcacccgctg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa cgcggcgcgg
      421 gcggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac gcctgcgcgc
      481 aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat ttgcagttca
      541 ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt caggatagct
      601 gtgagccaat ggcaattatc tttcgcccga cgctctccgg gatctaccag ccgcaactta
      661 atagccaact ttgcaacgga tgtggagcat gtgcagccag ctgtccggta tcagccataa
      721 ctgcggagta tctccatgca cactaactgg caagtttgca gcctggtcgt gcaggccaaa
      781 agcgaacgaa tttcagacat cagcacccaa ctgaacgcct ttcccggctg tgaagttgct
      841 gtcagcgacg cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga cagcgaaacg
      901 ctgatccaaa ccattgagtc agtacgcaac gtagagggcg tgctggcggt gtcgctggtt
      961 tatcaccagc aggaagagca aggtgaggaa acaccatgaa actcagtcgt cgtagcttta
     1021 tgaaagctaa cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg ccgggcgttg
     1081 cccgcgccgt tgttggtcag caggaagcca ttaaatggga taaagcgccg tgccgtttct
     1141 gcggtactgg ttgcggcgtt ctggtcggaa cgcagcaggg gcgtgtggtg gcctgtcagg
     1201 gcgacccgga cgcaccggtt aaccgtggcc tgaactgcat taagggctat ttcctgccca
     1261 agatcatgta cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa aacggtaaat
     1321 atgacaaaga aggcgaattt accccaatca cctgggatca ggccttcgat gtgatggaag
     1381 agaaattcaa aaccgccctg aaagaaaaag gaccggaatc gatcggtatg ttcggttctg
     1441 gtcagtggac tatctgggaa ggttatgccg cgtccaagct gttcaaagcg ggcttccgtt
     1501 cgaacaacat cgacccgaac gcacgtcact gtatggcgtc ggcagtagtt ggctttatgc
     1561 gtacctttgg tatggatgag ccgatgggct gctatgacga catcgagcag gctgacgcgt
     1621 ttgtgctgtg gggcgctaac atggcggaga tgcacccgat cctctggtca cgcatcacta
     1681 accgtcgtct ctctaaccag aacgtcaccg tggcggtgct ttctacctac cagcatcgta
     1741 gcttcgagct ggcggataac ggcatcatct ttacgccaca gtctgacctg gtgatcctga
     1801 actacatcgc caactatatc attcaaaaca atgcgataaa tcaggacttc ttcagcaagc
     1861 acgttaacct gcgcaaaggg gcgacggaca tcggctacgg tttacgtccg acccatccgc
     1921 tggaaaaagc agcgaagaat ccgggttctg acgcctccga accgatgagc tttgaagatt
     1981 acaaagcctt cgttgccgag tatacgctgg aaaaaactgc cgaaatgacc ggcgtgccga
     2041 aagaccagtt agaacaactg gcgcagctgt atgccgatcc gaacaagaaa gtcatctcct
     2101 actggacgat gggcttcaac cagcatactc gtggcgtgtg ggccaacaac ctggtctaca
     2161 acctgcacct gctgaccggc aaaatttccc agccgggttg cggtccgttc tccctgaccg
     2221 ggcagccttc cgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac cgtctgcctg
     2281 cggacatggt ggtgactaac gagaaacacc gtgatatttg cgagaagaag tggaatatcc
     2341 cgagcggcac cattccggcg aaaatcggtc tgcacgcggt ggcacaagac cgtgcactga
     2401 aagacggcaa gctgaatgtt tactggacca tgtgtaccaa caacatgcag gccgggccga
     2461 acattaatga agagcgtatg ccgggctggc gcgatccgcg caacttcatc atcgtctccg
     2521 atccgtatcc gacagtcagt gcgctggcag ccgacttgat cctgccgacc gcaatgtggg
     2581 tagagaaaga gggcgcttac ggtaacgccg aacgccgtac tcagttctgg cgtcagcagg
     2641 tacaggcgcc aggcgaagcg aaatcggatc tctggcagtt agtccagttc tcccgccgct
     2701 tcaaaactga agaagtatgg ccggaagagc tgctggcgaa gaaaccggaa ctgcgtggca
     2761 aaacgctgta cgaagttctg tatgccaccc ccgaagtgag caaataccct gtatccgaac
     2821 tggcggaaga tcagctgaac gatgaatccc gcgagctggg cttctatctg caaaaagggc
     2881 tgttcgaaga gtacgcatgg tttggtcgcg gtcacggtca cgatctggca ccgttcgatg
     2941 actaccacaa agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa acgcagtggc
     3001 gttacagcga aggtaacgac ccgtacgtga aagcgggcga aggctataag ttctacggta
     3061 aaccggatgg caaagcggtg atcttcgcac tgccgttcga accggcggcg gaagcaccgg
     3121 atgaagagta cgacctgtgg ctctctaccg gacgcgttct ggagcactgg cacaccggca
     3181 gtatgactcg ccgtgtgccg gaactgcacc gcgccttccc ggaagcggtc ttgtttattc
     3241 acccgctgga tgcgaaagcg cgcgatctgc gccgtggcga caaagtgaaa gtggtttctc
     3301 gccgtggcga agtgatctcg attgttgaaa cgcgcggtcg taaccgtccg ccacagggcc
     3361 tggtgtacat gccgttcttc gacgccgcac agctggttaa caaactgacg ctggatgcga
     3421 ccgatccgct ctcgaaagag acggacttca agaagtgcgc agtcaaactg gagaaggtgt
     3481 aagccattat gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt ctgcgcgatg
     3541 tcgttcgcac agcaggcggg ctggctgccg tgggtgtggc gctggggtta caacagcaaa
     3601 ccgcacgcgc atctggcgtg cggttgcgcc cgcccggagc cataaacgag aacgcctttg
     3661 ccagtgcctg tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac accttaaaac
     3721 tggcgacgct ggcctctggt ctgtcggcgg gcacgccata ttttgtcgca cgggatattc
     3781 cttgcgaaat gtgtgaggac attccgtgcg ccaaagtgtg cccaagcggt gcgctggatc
     3841 gtgagattga atcgatcgac gacgcgcgga tggggctggc ggtactggtg gaccaggaaa
     3901 actgtctcaa ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc ccgaaaattg
     3961 atgaggccat caccctggag ctggagcgca acacgcgtac cggtaagcac gcccgctttc
     4021 tgccgacggt tcacagcgac gcctgtaccg gttgcggtaa gtgcgaaaaa gtatgcgtgc
     4081 tggaacaacc ggcaatcaag gtgttaccgc tgtcactggc gaaaggggag ttaggtcacc
     4141 attaccgctt cggttggctg gaggggaaca atggcaaatc gtaaacgtga cgccgggcgc
     4201 gaggcgctgg agaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt acgtcgcctt
     4261 tgtcagttct tcgtgctggg gatgtttttg agcggtccat ggtttggtgt gtggatcttg
     4321 cacggcaact acagcagtag cctgttattc gacaccgttc cgctgaccga tccgctgatg
     4381 acgctgcaaa gtcttgccag tggtcatctg cctgccacgg tggcgttgac cggtgcggtg
     4441 attatcaccg tgctctacgc cctggcggga aagcgattat tttgcagctg ggtctgcccg
     4501 ctgaacccga ttaccgactt agcgaactgg ttacgcaggc gctttgacct caatcagtct
     4561 gcgacgatcc ctcgccacat tcgctacgtg ctgttggtgg tcattctgct gggatcggcc
     4621 ttaactggca cgctcatctg ggaatggatc aaccccgtat ctctgatggg gcgcagcctg
     4681 gtaatgggct tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt tgatctactg
     4741 gtcgttgaac acggctggtg cgggtacatt tgtccggttg gcgcgctgta tggcgtgctg
     4801 ggtagcaaag gtgtgattac cgttgcggcc accgatcggc agaaatgtaa ccgctgtatg
     4861 gattgttttc atgtttgccc ggaaccgcat gtgctacgtg ccccggtgct ggatgaacaa
     4921 agcccggtgc aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt ggatgtctgt
     4981 tctgaggatg tatttacaat aactacacga tggagttcgg gagcgaaatc atgaaaagcc
     5041 atgacctgaa gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg gtaagcggcg
     5101 cggtttgggc ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc gggacacagg
     5161 aaggggccat tcggatgcca aaagagcagg aacggatgcc gctgaactat gtgaatcagc
     5221 cgccaatgat cccgcatagc gttgaaggtt atcaggtaac gaccaatacc aaccgctgct
     5281 tgcagtgcca cggtgtcgaa agttatcgca ccactggcgc gccgcgtatc agtcctactc
     5341 actttatgga cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt tatttctgtc
     5401 tgcaatgtca cgtaccgcag gccgataccg cgccaatcgt ggggaatacc tttaccccat
     5461 caaaaggtta cgggaaataa gaggttatta tgggaaattc tgaccgtaag cctggtctga
     5521 ttaagcgcct gtggaaatgg tggcgtaccc ccagccgtct ggcgctgggg acgctgctgt
     5581 tgatcggttt tgttggcggc atcgtcttct ggggtggctt taacaccggg atggaaaaag
     5641 ccaataccga agagttctgc attagctgcc acgaaatgcg caacacggtg tatcaggaat
     5701 acatggattc cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt ccggattgtc
     5761 acgttccgca cgagtttgtg ccgaagatga tacgcaagct caaagcaagt aaagagctgt
     5821 atggtaaaat ttttggcgtt attgacacgc cgcagaaatt tgaagctcat cgtctgacga
     5881 tggcacagaa tgagtggcgg cgcatgaagg acaataactc gcaggagtgc cgtaactgtc
     5941 acaacttcga gtatatggat acaaccgccc agaaatcggt tgccgcgaag atgcatgacc
     6001 aggcggtgaa agatgggcaa acctgtattg attgccataa agggatagcg cacaagctgc
     6061 ccgatatgcg tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt atgcttgaag
     6121 ccagagagtt actttgtgag cgggatgaac gaaccttatt tagtggcttg tcatttacgc
     6181 tgaacgcagg agagtgggta caaatcaccg gtagcaacgg cgcggggaag acaacgcttc
     6241 tccgtttgct gacggggttg tctcgccctg acgcaggcga ggttctctgg caagggcagc
     6301 ccttgcatca ggtacgcgac agctaccatc aaaacctgtt atggataggc catcagccgg
     6361 ggatcaaaac ccggctgacg gcgttagaaa atctgcactt ttatcatcgc gatggcgata
     6421 ccgcacaatg tctggaagcc ctggcgcagg ccgggcttgc cggattcgaa gatattcctg
     6481 taaatcagct ctcggccggg caacaacgcc gcgtcgcttt agcgcgtctg tggctgaccc
     6541 gtgccacgtt atggatcctc gacgagcctt ttaccgcgat tgacgttaac ggcgtcgatc
     6601 gtctgaccca gcgtatggcg cagcatacgg agcagggggg gattgtgatt ctgactaccc
     6661 accagccgct caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg caaacgaggg
     6721 ccgcatgatg ttctggcgca ttttccgtct tgagctgcgt gtagcgtttc gccatagcgc
     6781 cgaaatcgcc aacccgctgt ggttcttcct gattgtaatt accctttttc cgctcagtat
     6841 cggtccggag ccgcaactgc tggcgcgtat tgcaccgggc attatctggg ttgctgcact
     6901 gctttcatcc ttgctggcgc tggaacgact gttccgtgac gatttgcagg acggcagtct
     6961 tgaacaattg atgttgttgc cgttaccctt gcccgccgtt gtgctggcga aggtgatggc
     7021 gcactggatg gtaagcggtc tgccgttact cattctttcg ccactggtag caatgctact
     7081 gggaatggat gtttatggct ggcaagtgat ggcgctgacg ctgctgctgg gaacgcctac
     7141 gcttggcttt ctcggtgcac cgggcgtggc gctgacagtg ggacttaagc gcggtggtgt
     7201 gctgctcagc atactggtgt taccgctgac tatcccatta ctcatcttcg ccaccgccgc
     7261 gatggacgcg gcttccatgc atttgcccgt tgacgggtat ctggcaattt taggcgcgtt
     7321 gctggcaggc accgcgacat taagtccttt tgcgacggcg gcagcgttac gaatcagcat
     7381 tcaataacgc tggattactg atttttgttc gagtctggta tcgaaactat gtggaaaaca
     7441 ctgcatcaac tggcgatccc accacggctg tatcaaatct gtggctggtt tataccgtgg
     7501 ctggcaattg ccagtgtggt cgtgcttacc gtcggctgga tctggggatt cggctttgct
     7561 ccggctgatt atcagcaggg aaatagctac cgcattatct acctgcatgt gcctgcggcg
     7621 atctggtcga tgggcattta tgcatcaatg gcagtggcag cgtttattgg ccttgtctgg
     7681 cagatgaaaa tggccaacct ggcggtggcg gcgatggccc ccattggtgc cgtgtttacc
     7741 tttattgccc tggttaccgg ctctgcatgg ggaaaaccga tgtggggcac ctggtgggta
     7801 tgggatgcac gtctgacttc tgaactggtg ctgctttttt tgtatgtggg tgtgattgcc
     7861 ctgtggcacg ccttcgacga ccgccgtctg gcgggccgtg cggcaggtat cctggtgctg
     7921 attggcgtgg tgaatctgcc gattattcat tactccgtgg agtggtggaa caccctgcat
     7981 cagggatcaa cgcggatgca gcaaagtatc gatccggcga tgcgttcgcc gctgcgctgg
     8041 tcgatttttg gcttcctgct cctgtctgcc acgctgacgc tgatgcggat gcgtaatttg
     8101 attttgctga tggaaaaacg ccgtccgtgg gtgagtgaac tgatactgaa aagaggccgt
     8161 aaatgacccc tgcatttgct tcctggaatg aatttttcgc aatgggcggt tacgcctttt
     8221 ttgtctggct ggcggtggtg atgaccgtta ttccgctggt ggttttggtc gtgcactcgg
     8281 tgatgcagca tcgcgcaatt ctgcgtggtg tggcgcaaca gcgggcgcgt gaggcgcgtt
     8341 tacgtgctgc gcaacagcag gaggctgcat gaatattcgc cgtaaaaacc gcttgtggat
     8401 tgcctgtgct gttctggcag ggttggcact gactatcggt ctggtgctat atgcgctgcg
     8461 ctcgaatatc gatctctttt atacgccggg ggaaattctc tacggcaagc gtgaaactca
     8521 gcaaatgccg gaagtcggtc agcgtctgcg cgttggcggg atggtgatgc cgggtagcgt
     8581 gcagcgcgat cccaattcgc taaaagtaac tttcaccatt tacgacgctg aaggttcagt
     8641 agatgtctct tacgaaggca ttttgccgga tctgttccgt gaagggcagg gcgttgtggt
     8701 gcagggcgaa ctggaaaaag gcaatcatat cctcgcaaaa gaagtgctgg cgaaacacga
     8761 cgaaaactac acgccgccag aagttgagaa agcgatggaa gccaatcacc gtcgcccggc
     8821 gagtgtttat aaggacccag catcatgatg ccggaaattg gtaacgggct gctgtgtctg
     8881 gcgctaggaa ttgcgctgct gctgtccgtg tatccgctat ggggcgtagc gcgcggagat
     8941 gcgcgcatga tggcgtcttc ccgcttgttt gcctggctgc tgtttatgtc tgtggctggc
     9001 gcatttctgg tactggtcaa tgccttcgtg gtcaacgact tcaccgtcac ctatgttgcc
     9061 agcaactcca atacccagct tccggtgtgg tatcgcgtgg cggctacctg gggcgcgcat
     9121 gaaggctcgc tcctgctgtg ggtgctgctg atgagcggct ggacctttgc ggtagcgatt
     9181 tttagtcagc gtattccgct ggatattgtg gcccgcgtac tggcgataat ggggatggtc
     9241 agcgtcggct ttttgctgtt cattctcttt acctctaacc cgttctcacg cacgttgccg
     9301 aacttcccga ttgaagggcg cgatcttaac ccgctgttac aggatccggg gctgatcttc
     9361 catccgcctc tgctctatat ggggtacgtg gggttctcgg tggcgtttgc ttttgccatt
     9421 gcttctttgt tgtgcgggcg tctggacagc acttatgcgc gttttactcg tccgtggacg
     9481 ctggcggcgt ggatcttctt gacgctcggc attgtgctcg gttccgcatg ggcctattac
     9541 gaactcggct ggggcggctg gtggttctgg gatccggtag aaaacgcctc gtttatgccg
     9601 tggctggtgg ggactgcgct gatgcactca ctggcggtca ctgaacaacg cgccagcttc
     9661 aaagcgtgga cattactgct ggcaatcagt gccttctcgt tgtgtctgct ggggactttc
     9721 ctggtgcgtt ccggcgtgct ggtatcggta cacgcgtttg cctctgatcc ggcacgcggt
     9781 atgtttatcc tcgcctttat ggtactggtg attggtggtt cgctgctgct gtttgccgcg
     9841 cgtggacaca aagttcgctc acgcgtaaac aatgcgctgt ggtcgcggga atctctgctg
     9901 ttagcgaaca acgttttgct ggtcgccgcg atgctggtgg tattactggg gacgctgctg
     9961 ccgctggtgc acaagcaact gggactgggc agtatttcga ttggcgaacc gttcttcaac
    10021 accatgttta cctggctgat ggtgccgttt gcgctgctgc ttggtgtcgg tcctctggtg
    10081 cgctgggggc gcgatcgccc acgtaaaatc cgcaatttat tgattatcgc cttcatctca
    10141 acgctggtgc tgtcgctgct gctgccgtgg ctgttcgaaa gcaaagttgt ggcgatgacg
    10201 gtgctcggcc tggcaatggc ctgctggatt gctgtgctgg caattgcgga agctgcgctg
    10261 cgtatttcac gtggcacgaa aaccaccttc agttattggg gaatggtggc ggctcacctg
    10321 gggctggcag tgacaattgt tggtattgcc tttagccaga actatagcgt tgagcgtgat
    10381 gtgcgcatga agtccggcga tagcgtcgat attcatgaat atcgcttcac cttccgtgat
    10441 gtcaaagagg tgactggccc gaactggcgt ggcggtgtgg cgactatcgg cgtaacgcgc
    10501 gatggcaagc cggaaacggt gctgtatgcg gaaaaacgtt attacaacac tgccgggtcg
    10561 atgatgaccg aagcggcaat tgacggcggc atcacgcgtg acctgtacgc cgcgctcggt
    10621 gaagagctgg aaaacggcgc gtgggccgtg cgtctttact acaaaccatt tgttcgctgg
    10681 atttgggcgg gcgggctgat gatggcgttg ggcggactac tgtgtctgtt tgatcctcgc
    10741 tatcgtaagc gcgtgagtcc gcaaaaaact gcgccggagg ccgtatgaag cgcaaagtat
    10801 tgttaattcc gttgattatc ttcctggcga ttgccgcggc gctgctgtgg cagctggcgc
    10861 gtaatgccga aggggatgat ccgaccaatc tggaatcggc gctcattggc aagcctgtgc
    10921 cgaagtttcg tctcgaatca ctggacaatc cggggcagtt ttatcaggcg gatgtgctga
    10981 ctcagggcaa accagtactg cttaacgtct gggcgacctg gtgtccgacc tgccgtgcgg
    11041 aacatcaata tctgaatcag ctttcagcgc agggcattcg cgtggtcggc atgaactata
    11101 aagacgatcg ccagaaggca atcagctggc tgaaagagct gggcaatcct tacgcgctaa
    11161 gcctgtttga tggcgacggc atgttagggc tggatctcgg tgtctatggc gcgccagaaa
    11221 cgttccttat tgacggcaac ggcatcattc gctatcgcca tgcgggcgat ctcaatcctc
    11281 gcgtctggga agaagagatc aagccgctgt gggagaaata cagtaaggag gccgcacaat
    11341 gaggttttta ttgggcgtgc tgatgctgat gatctccggc tcagcgctgg cgaccatcga
    11401 cgtgttgcag tttaaagatg aagcacagga acaacagttc cgtcagctca ctgaagaact
    11461 gcgctgcccg aaatgccaga ataacagcat tgccgattcc aactcgatga ttgccaccga
    11521 cctgcgccag aaagtgtatg aactgatgca ggaaggtaaa agtaagaaag agattgtcga
    11581 ttatatggtg gcgcgttacg gcaacttcgt cacttacgat ccgccgttaa cgccgctgac
    11641 cgtgctgctg tgggtgctgc cagtagtggc tattggcatt ggcggttggg tcatttacgc
    11701 ccgctcgcgg cgtcgggtac gcgtggtgcc ggaagcgttt cctgaacaaa gcgtgccgga
    11761 aggtaagcgt gccggatatg tcgcttattt gccgggcatt gtggtggcgt taattgtggc
    11821 tggcgtcagc tactaccaga ccggaaatta tcaacaggtg aaaatctggc agcaggccac
    11881 ggcacaggct ccggcgttgc tggacagggc gctggatccg aaagccgatc cgctcaacga
    11941 agaagagatg tcgcgtcttg cgctggggat gcgtactcaa ctgcaaaaaa atccgggaga
    12001 tatagaaggc tggattatgt tgggccgcgt tggcatggcg ctgggtaacg ctagtatcgc
    12061 caccgatgca tacgctactg cgtatcgcct cgatccgaaa aacagcgatg ccgcactggg
    12121 ttatgctgaa gcgttgacac gttcatctga tcccaacgac aaccgcctcg gcggcgaact
    12181 gctgcgccag ttggtgagaa gcgaccacag caatatccgt gtgttaagca tgtatgcgtt
    12241 taatgccttt gagcagcagc gatttggcga agccgttgcc gcgtgggaga tgatgttgaa
    12301 actcttacct gccaacgata ctcgccgtgc ggtgattgaa cgtagtatcg cgcaggcgat
    12361 gcaacatttg tcgccgcagg agaataaata aaaagaggcc cgatggtgat gccatcgggc
    12421 tatttttatt gtgccccgcg ttgttgcagg aacagaatgg tggctgccac gcgtgagcgg
    12481 acattgagtt tgcgcagcag attgcgaata tgtactttta ctgtctgctc tgaaatattc
    12541 aacaccgagg caatctgttt atttgacagc ccctgtgcca gctcgtgcag aacatccagc
    12601 tcgcgctccg tcagcacgct gaagggatct tcttccgcgc caaacatttc acgttcacgt
    12661 aagtactgat tgacgcgttc gctaaagact ttgctgcctt tcgctccagc acgaattgct
    12721 tccagcaata cttccgggtc gctgtctttc aacagataac cgtctgcgcc tgcgtctatc
    12781 agcgcaaaga catcgctgga ggcatctgat acggtcagga taataatttg cgcggtaacg
    12841 ccatccctgc gcaaggcatt gagggtatcc aggccactca tacctttcat attgagatcc
    12901 agcaagatca cgtcgatatc cagtctattc gccagatcga tcgcgctcgc gccgtcgccc
    12961 gcttcggcga ccacttcaaa gccaggatca agctccagta actgacgaac accgcgtcgc
    13021 ataagtggat gatcatccac aatcatcacc tgaaaaggcg ttgcttcagg catagtagtc
    13081 tcctgaggtt ttattagaat cattatgttt gagcgtagcg ttggtaacgc taccaggcgc
    13141 gtgaatgtta ccccatttta tgtatggaca tgtagtgaaa actacaacct tgtcgttcgc
    13201 tttggcatca ttttcattat aaagacggaa ttttgcatca gcttctcatc aagaaaaaca
    13261 actaaaaaat caatcacaat acttgtgcat ttctttaaac agtttttaaa aactgactcc
    13321 cggtatggag ctatgggtat tttctgtacc caatgctttt aacagcaatt aatttcatag
    13381 gatgaaagct caatgcatca atctggttct gtttctcttt gtcgttccgc aatatctgtt
    13441 ctggtggcta cagcgttata ttcacccata gcattggcat caacagttga gtatggtgag
    13501 acagttgatg gtgttgtcct ggaaaaagat atccagctgg tttatgggac cgccaataat
    13561 acgaaaatca atcctggcgg agaacagcat ataaaagaat ttggtgtaag taataatact
    13621 gaaattaacg gagggtatca gtacattgaa atgaatggcg ccgcagaata ctcagtatta
    13681 aatgacggtt atcaaattgt tcaaatgggt ggcgcggcaa accagactac gctcaataat
    13741 ggtgtgctac aggtttatgg cgcagcgaat gataccacga ttaaaggcgg gcgcttaatc
    13801 gttgaaaaag atgggggggc cgtctttgtc gctatcgaaa agggaggact actggaggtt
    13861 aaagaggggg gattagcgat tgcggtagat cagaaagcag gcggtgctat taaaacaacc
    13921 acgcgggcca tggaggtatt cggaacaaac cgtctcggtc agttcgatat caagaatggt
    13981 attgctaata atatgttgtt ggaaaacggc ggaagtttgc gagttgaaga aaatgacttc
    14041 gcttataata ccactgtaga tagtggcggc ttactggagg ttatggatgg cgggactgta
    14101 actggcgttg ataaaaaagc aggcggaaaa ttaattgtct caacgaatgc gctggaagtg
    14161 agtggtccaa acagtaaagg ccaatttagt ataaaagatg gtgtgtcaaa aaattatgaa
    14221 ctggatgatg gttccgggct cattgttatg gaggacacgc aggccattga tactatcctt
    14281 gatgagcatg ccactatgca atcgctggga aaggatactg gtacgaaagt gcaggcaaat
    14341 gcggtatatg atctcggtcg atcatatcag aatggaagta tcacgtattc ctcaaaagcc
    14401 atctctgaaa atatggttat caacaatggc cgcgctaacg tctgggctgg cacaatggtt
    14461 aacgtttcag tcagagggaa tgatggcatt cttgaggtca tgaagccgca aataaattat
    14521 gcacccgcaa tgttggtggg taaggtagtg gtttctgagg gcgcttcttt tagaacgcat
    14581 agtgccgtgg ataccagcaa agcggacgtt tcgctcgaaa atagcgtatg gaccatcatt
    14641 gccgatatca ctacgacgaa ccaaaacacc ctcctcaact tagccaacct tgcgatgtct
    14701 gacgcaaatg tgattatgat ggatgagcca gtgactcgtt catcagtgac ggcaagtgcg
    14761 gaaaatttca ttacgttgac caccaatacc ctgtcgggaa acggcaattt ttatatgcgt
    14821 accgatatgg ctaatcatca gagcgatcag ctcaacgtca ccggtcaggc aacaggtgat
    14881 ttcaaaatat tcgtgacgga caccggtgcc agcccggcag caggagatag ccttacactg
    14941 gtaacaacgg gcggcggtga tgctgcattt acgttgggca atgccggagg cgttgttgat
    15001 atcggtacgt atgaatatac cttgctggat aatggcaacc atagctggag tttggcagag
    15061 aatcgcgcgc aaattacccc ttcaaccact gatgtgctga atatggcggc agcacaaccg
    15121 ctggtatttg atgcagaact ggacaccgtg cgtgagcgtc ttggtagcgt aaaaggcgtt
    15181 aattacgata cggcgatgtg gagttcggca attaacaccc gcaacaacgt gaccactgat
    15241 gcgggcgctg gctttgagca aacattgacc gggctgacgc tcggtatcga tagccgtttc
    15301 tcccgtgaag aaagcagcac aattcgcggc ttgttctttg gttactctca ttctgatatt
    15361 ggttttgatc gcggcggcaa aggcaatgtc gatagctata ccctgggggc ttatgccggt
    15421 tgggagcatc agaacggtgc ctatgttgat ggagtggtga aagttgaccg ttttgccaac
    15481 accatccatg gcaagatgag taatggggca acagcgtttg gcgattacaa tagtaacggc
    15541 gcgggtgctc atgtcgagag cgggttccgt tgggttgacg gattgtggag tgttagaccc
    15601 tatctggctt ttaccggctt taccacagat ggtcaggact acacgttatc aaacggcatg
    15661 cgcgcggatg tgggaaatac ccggatatta cgcgctgaag cgggaacggc ggtaagctat
    15721 cacatggacc tgcaaaacgg tacgacgctg gaaccctgac tgaaagccgc cgtgcgtcag
    15781 gaatacgccg attctaacca ggtgaaagtt aatgacgatg gcaaatttaa taatgatgtg
    15841 gctggaaccc gtggcgttta tcaggctggg ataaggtcat cgtttacccc gacgttaagc
    15901 ggtcatttgt cagtcagcta tggcaatggc gcaggggtag aatcgccgtg gaatactcag
    15961 gcgggtgtgg tctggacgtt ctgataacaa aaaataaaca ggctgtgatg tgtcacggtc
    16021 tgtttatcga attaattgca gatataaaaa aaaccaaccg taaaggttgg ttttttcttg
    16081 ggatttttgg cacgagagta gtttaattga gtatgtaact acatgatttt aaatagtatg
    16141 cttgattcaa cttttaaacc gtataactaa acgtataact aaaatcattt gctgaaaaag
    16201 tgtacaattt aaatgcgagt tttcctgtgg taaaagctcc aaccgtaacg atgttcgtta
    16261 tggttgcctt gatggtctgc gtggtcatta tgaccattca ggatttgaag ggtgacgctt
    16321 ccgacttcct caaattgagg tttccgaaaa tatcagcggg ttagcgatta atctgaccat
    16381 taattaaact gcgaaaattt cgtaattatc ccgatatggg gatcaccata tcgggattga
    16441 tgccggaaaa atcagctttt cgccagaacg gtgacggcct tatcgagttt ttgccatccg
    16501 caataggtga ttgccctgaa cgctgtagcg tcattctgga aaaggctaac cgtccctgat
    16561 tcggttttta ttaccgcctc atcagttggg gttataaccg tggcttcaac gttaataatc
    16621 agcttttgag cgtctatcag catcatgcga tccgcctgtt cgctcacaat taccggtatt
    16681 ctcaaaagct ctatctgcca gaactcagca tcagtaagct gtaaggcgtt ttctacgggc
    16741 aagattagcg acgacgtggc aagcgtgtta ccgttcgcta tgtgcttttt gaaaagctct
    16801 atgaagtttt cggcctcatc agcaccatta agcacgccct caggggatac gcccgccacc
    16861 tcctggtcac tggaaagaaa ttttttatct atttctttca ccgattccga tgtgatcaag
    16921 tctctgatcg ctgaattggt accaggggtg ctaaatttga ttagctcgtt attcagcatc
    16981 acaatagcgc caagtttgca cagttttagc gcactttcgt ttacttctcc ctctttgaca
    17041 gggatttctt cggcttcttt aagccattta ctacccttgc ctgatattga ctgcaatttt
    17101 gcgccaaacg gaagattcag cgcgctgctg attagcttgc ctggtacaga ctgcgaaaaa
    17161 atatactcgc caagctctct tactatcggc tctgccacca gcgacggcat attatccgta
    17221 gtcattccgg caacaatgga tttttcaaga aatttcgata ctgccggatc attttttttc
    17281 gtcatttcaa ttgcgtctgc atggctgaat ttacacgatg taagcacgct ggcggcgcga
    17341 actgccgaca cgcctttcat gtttttagaa atcattatct tcttcctcct ggtaacatgg
    17401 ggttagggtt tcgtgaaaat atggcatcag aacagcggca gcctgtaacc gtatttcggt
    17461 tggctgctgc ttgtcgttca tgatttccgt gagtacatca atagggtctg tgatgtattc
    17521 gctcatagtt cgctacctca gtgttttaag ccagttggtg gcttagggat ggggaaccga
    17581 tttgaggaga cattagcggc attgagtttt ttaacctctt ttttcccaat gctgccgatc
    17641 ttgcggtggg tgaactgggc cagcttaaac gcggcatcta aagccagttt tggatctgtg
    17701 tccatgttgt caatcagtat cctgcccatt gctttgatgg ggtcgggtag gccgttatcc
    17761 atcatctcaa tggtgttaat tgatgcccgt tccggtgtaa ctttttcggt aactttcttt
    17821 gtaacttttt tcggttttgt aacctgaaaa gttacatcag aagttacagc cttaagatac
    17881 tttttcacct ccggatcatt ggatagctgc cagcctttaa cccttgcagt cttttcgctg
    17941 tatcccgcat gtatcgcggc ttccgttgga tttgtgccct ttgataacgc atcagcaaac
    18001 cgcttttttt tggtcgttaa tgccatgcaa aaacctcgtt ttgagggggt gtaacttttc
    18061 gtgtaacttt tttgaaaagc aaaattttct atgaatgaga ctgggggcgg tgtccagggc
    18121 gatcgacttt ccggccggaa cactcccccc ctgtcattgc tcaccgagta acgagatcag
    18181 gccttcaact tcatcactca gcttgtttat tcgtgtctca agctctgcga tcttttccat
    18241 caatggcgct atggtgtctc ttgtttcctc gccaatggct ttaatgatgc ttgcctcgcg
    18301 ttctgtgata gccattgttt acgcctcact tgttgcaatg cccaggcgtt cgcgtaacag
    18361 tacgcgcgca agatgcccta ttgttggtgt tgccgccctg ccgtagatac ctgattcatt
    18421 catccggcgc acaatatcga actgcaactt tttaagctcg ttaaggattt catgatcaat
    18481 catcacggta gcgctttgct ttatctcttc cactttgttt tctcctgata cttatcattg
    18541 cataaccagt attttcgata gattatcatt aattaaccat ctttgaagat atatgagatc
    18601 gaaaaaaagc cggattgctc cggctatgtg ctcacttctt cggttattct ccagcgcttt
    18661 taacgttgat ggttgttacc tgttccgctt cggcaatctc ccgttctgtc agcgtggcaa
    18721 agtttgccgc cgctgtggtc atgaatgcgc ttatcagttc gggatgctcc ttcgcgtatc
    18781 cttcccccgc gtggcggtct atcgttctga ttgccacctt taaggaaagc tctgtcatgt
    18841 ctaacgcttt atattttggc gctgtcttat ctctggtttt tctgttcatt ccccactact
    18901 ccccactttt gctccccact ttttgaaatt acccaccgtc tccccacctc ttttttttgt
    18961 gattctaacg ttatgttttt caatatgttt tttactcccc acttttttgg gtatatacag
    19021 gtgggaaagt gggtaattat gtttcaattt tgttaaattc cccactctcc ccacttttac
    19081 tccccacttt ttacagcggg cgcacgtcat caccgtcaat aacgattacg ccatcatttt
    19141 ccagcttgta cagccagcgc cggaagtgct tcatctcata ccccagcttt ttcatatcgt
    19201 cgcggagaag ggcgatagtg cacgcctccc cgtgtgctgt tcgtgttctg atgcactgcc
    19261 atagcgcggc gtgattctcc gtcttgttcc ctgcctcctc gatgcgctcc agttcaacgg
    19321 gagggcgcgg cttatccacc accaccagcg acgtgattaa ctccccgtca gcgtcggtaa
    19381 aaagctc
//
LOCUS       DAZFDP010000088        19373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFDP010000088 DAZFDP010000000
VERSION     DAZFDP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..1007)
                     /locus_tag="ACHJ1G_003155"
     CDS             complement(138..1007)
                     /locus_tag="ACHJ1G_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4764959.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHJ1G_003156"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHJ1G_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4764960.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="ACHJ1G_003157"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="ACHJ1G_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4764961.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHJ1G_003158"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHJ1G_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4764962.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHJ1G_003159"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHJ1G_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4764963.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="ACHJ1G_003160"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="ACHJ1G_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4764964.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHJ1G_003161"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHJ1G_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4764965.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="ACHJ1G_003162"
     CDS             complement(4585..5718)
                     /locus_tag="ACHJ1G_003162"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4764966.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHJ1G_003163"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHJ1G_003163"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4764967.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHJ1G_003164"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHJ1G_003164"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4764968.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHJ1G_003165"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHJ1G_003165"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4764969.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHJ1G_003166"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHJ1G_003166"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4764970.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="ACHJ1G_003167"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="ACHJ1G_003167"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4764971.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHJ1G_003168"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHJ1G_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4764972.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHJ1G_003169"
     CDS             12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHJ1G_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO4764973.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            12478..12792
                     /locus_tag="ACHJ1G_003170"
     CDS             12478..12792
                     /locus_tag="ACHJ1G_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO4764974.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHJ1G_003171"
     CDS             complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHJ1G_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4764975.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHJ1G_003172"
     CDS             complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHJ1G_003172"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4764976.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHJ1G_003173"
     CDS             complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHJ1G_003173"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4764977.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            18378..18923
                     /gene="orn"
                     /locus_tag="ACHJ1G_003174"
     CDS             18378..18923
                     /gene="orn"
                     /locus_tag="ACHJ1G_003174"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4764978.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            19134..19209
                     /locus_tag="ACHJ1G_003175"
     tRNA            19134..19209
                     /locus_tag="ACHJ1G_003175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19167..19169,aa:Gly,seq:gcc)
BASE COUNT         4491 a         4938 c         5071 g         4873 t
ORIGIN      
        1 caggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg ttggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttctta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttggcta tataaagagt aacaacagca atgtatatga ataattaaag
     1201 gttatgcaac gggcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgcc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc
     4981 catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac
     5041 atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt agaacccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 acgccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgct ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 cgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ccggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagca gagtggcaaa aaatccgcag tgggcggtgg aataacccga
    12121 tgccctatcg tctacctgac gatagggcgt aaagacagga aatgttatgg atgacaccag
    12181 ccgggacccg gcaataaccg aagatgaaat cagggcgctg caattttctg ccggggatgt
    12241 agcggaaata gagcagacga ttctctcttt tgtcgatgcc tgccacaccc gtaaagtggc
    12301 gatggtggtg ggtagcacca ttaacaccct caaagatcgc gatggtaaac ggtggggaaa
    12361 cctgccggat atttattgcg cttatcttat ccgatgtctg gtttttcgcg gggaattagt
    12421 gggatacggc gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg ttaccttatg
    12481 agtgcgcgca tgtttgtctt atgctgcatc tggtttattg tggcgttcct ctggataacc
    12541 atcacttccg cgctggacaa agagtggatg attgatggac gcgggattaa caacgtctgc
    12601 gatgtgttga tgtatctcga agaagacgat acccgtgatg ttggtgtgat aatgacgtta
    12661 ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg ctggtttatg
    12721 tacgccaccg cgctggctat tttcggctac tggctgtggc agttttttct gcgctatcag
    12781 ttttgtttgt gagccggatt ggttcatccg gcacacaaac ttacaaactt cccgcctgac
    12841 gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac
    12901 gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca
    12961 gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca
    13021 gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt
    13081 cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcgctat
    13141 tggcatcggc aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc
    13201 agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc
    13261 agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggta agatcgcgga
    13321 tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcagg ccagagataa
    13381 agttggcgaa aatctcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc
    13441 actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg ccaatcagca
    13501 tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt aaatccaggt
    13561 gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga
    13621 taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga ctttctacgc
    13681 cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat
    13741 ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa attgagcgca
    13801 ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa
    13861 ttgccccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgcg cgttgcgcta
    13921 acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca
    13981 tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt
    14041 caagcctcgc taacagtgcc tgcgccgttg ccagataacc caccgctgac gccagaatgg
    14101 caaccagtgg tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc
    14161 tgccctcttt gttgaggtac agcgggatcc cggccttttt caggctgagg gtgaccaccg
    14221 ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt
    14281 cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca
    14341 tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa
    14401 acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg
    14461 tggccgttac gccatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc
    14521 cgtaacccag cgtcatccac agcaccggca gcggtgacgc gacgagaatc gaccagaaaa
    14581 gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac
    14641 gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg
    14701 cgccaaacag cggcaaaatc gtctctttgc tggtcagcat catcacactg gctttgccca
    14761 actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagtggcc
    14821 aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcgatgc gtcgcttcat
    14881 tcacctcttt cagcgcatcc tcaagttgcc cgttggagac tttcagcttg gtcagttcca
    14941 gcagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct
    15001 gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatgaattt
    15061 gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc acacgcaact
    15121 gcgccatttc ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt gccacctgcg
    15181 cccgcaacgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg
    15241 tattcagcgc ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt tgcgaggcaa
    15301 cgagatccat ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg ttaattttga
    15361 attgcgcgac gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat tcggtacttt
    15421 ccagcgcccg ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca
    15481 aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt
    15541 cctgacggtt attggcagac agctgcgcca gttccagttc atcaacgagc gctttcaggc
    15601 gtgcggagtc agactgcaac gcgaaatttt gtgcctgatt gagcggggta ttgccagtaa
    15661 gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg
    15721 gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac
    15781 ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag
    15841 acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt gcgcgcagag
    15901 tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg
    15961 aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct
    16021 gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg
    16081 ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc
    16141 gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc
    16201 tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg
    16261 taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt
    16321 ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg gtagagccga
    16381 gtttaaagcg gcccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg
    16441 caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga
    16501 ccgtctcaat gctgcctaca atcgtcgccc cgaccagaat ctgcgccatc gggccaaatt
    16561 cggtatcgaa caggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag
    16621 cggtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca cgcagaatac
    16681 cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg
    16741 taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt
    16801 agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa
    16861 cgccatcggc aggcatgacc aggacattcg gatcggtatc gattgggcgt acttcgtcac
    16921 gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg
    16981 cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc
    17041 atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa
    17101 tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc
    17161 gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt
    17221 agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc
    17281 tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg
    17341 cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac
    17401 ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga
    17461 ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca
    17521 gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca
    17581 ggatctcttt ttgcagcccc agcaacgcat tcagcaggct ggatttgccg acgccagact
    17641 gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt agcccatcct
    17701 gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata tccatctgct
    17761 cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acaataatcg
    17821 gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct
    17881 ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata ggttttacgc
    17941 cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct tccacgatcc
    18001 ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg
    18061 ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt
    18121 ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca
    18181 gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt
    18241 tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt
    18301 cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg
    18361 aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga
    18421 tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg
    18481 ccaacctgaa tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc
    18541 tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg
    18601 tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa tttttaaaac
    18661 agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggtcag gaccgtcgtt
    18721 tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca
    18781 gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc
    18841 aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc
    18901 gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg
    18961 cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata
    19021 aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc
    19081 ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa
    19141 tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt
    19201 cccgctccaa aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa
    19261 ttgcgacaag gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt
    19321 gagtgaaggc agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaa
//
LOCUS       DAZFDP010000089        18929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFDP010000089 DAZFDP010000000
VERSION     DAZFDP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..245)
                     /locus_tag="ACHJ1G_003176"
     CDS             complement(<1..245)
                     /locus_tag="ACHJ1G_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4764979.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRL"
     gene            complement(300..812)
                     /locus_tag="ACHJ1G_003177"
     CDS             complement(300..812)
                     /locus_tag="ACHJ1G_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4764980.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(812..1996)
                     /locus_tag="ACHJ1G_003178"
     CDS             complement(812..1996)
                     /locus_tag="ACHJ1G_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4764981.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCDTMADAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            2154..3257
                     /locus_tag="ACHJ1G_003179"
     CDS             2154..3257
                     /locus_tag="ACHJ1G_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO4764982.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAAAFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAE"
     gene            complement(3422..4057)
                     /gene="hxsA"
                     /locus_tag="ACHJ1G_003180"
     CDS             complement(3422..4057)
                     /gene="hxsA"
                     /locus_tag="ACHJ1G_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser repeat protein HxsA"
                     /protein_id="HFO4764983.1"
                     /translation="MKKFNFAALLPGFLALNNSVWASDSSTGASDLPGMTLNEHDLVI
                     APLNTEVPFYIAGHRSHSSHRSHSSHRSSSGGGYYGGSTPYYPKTYSSPRSSGSSSSG
                     ASSSSSSSSVRALRSNDNNRTTNTSSVGTSEVNRTSNGLASDAEKRKRLIMRVQFALL
                     DRGFYNGNIDGIMGPSTRLSVKNYRIANGLPIPATETLDTQLLNSLNILAR"
     gene            complement(4054..5166)
                     /gene="hxsC"
                     /locus_tag="ACHJ1G_003181"
     CDS             complement(4054..5166)
                     /gene="hxsC"
                     /locus_tag="ACHJ1G_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsC"
                     /protein_id="HFO4764984.1"
                     /translation="MSEVIRNDIFHFASTENVPTGFYRLCKQKPVNPLFFLPNLLVVA
                     EGNNDAIPPCFDFSVISTELFESIEDGDIGIINNGNMIRVILSRKANHNTVLVTERCN
                     NLCLFCSQPPKKINDDWLLTQSALAIASFGLNGVVGVSGGEPLLYGDDFLHFIDFIIE
                     NSPDTALHVLTNGRKFADINFTQEMAKRSKKIKITFGIPLYSSRPLVHDHLVGSDGAF
                     NETVKGLINAGNLGINIELRVIPTLANYTELDDIVEFAGRVFSNINQISLMGLEPIGW
                     ARKNWSTIFIDHSSYSEKIISAIDAAYRSGIPLTIFNYPLCYLPERARELAVQSISDW
                     KNYYPKECDECTQKPSCAGYFSSSKGRFHQSPRPIL"
     gene            complement(5159..6547)
                     /gene="hxsB"
                     /locus_tag="ACHJ1G_003182"
     CDS             complement(5159..6547)
                     /gene="hxsB"
                     /locus_tag="ACHJ1G_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HFO4764985.1"
                     /translation="MELMPFNFDRLPNGQVFISNLAGFHHFIGEQDLIDLSDERISSE
                     QSNVLESKLFITSESSSAITPYALSSAFAKRLMNELAVRPIFMIVPTLRCDHTCKYCQ
                     VSRASVNASGYDLNPELIPDIISAIKKLSTPPYKIEIQGGEPLLRFDLIQSIYDQCAE
                     TLGRTNFEMVVASSLSVLNEDMIEWSRDRNITFSVSLDGEEVVHNSNRILGRGLAYSR
                     TVSGVEAIKHSLGAGRVATVTTVTKELIKRPESIVQAHLSLGLKDMFIRPVSPYGFAQ
                     KASFTFSMDEYFSFYASLIDEILKINNEGIRVVEHSASIHLKRIFNPGFSGYADLKSP
                     SGVVLNSILFNYDGRVYGSDESRMLQKVNPETEFSAGEVKTLSFSDSPYYNAVLSSSF
                     NFALPGCDTCAYQPFCGSDPCQNISVQGEPVGDRSRSTFCQYHKGMFRYLMNCISEGG
                     SKAEMLKGWAYV"
     gene            complement(6547..6819)
                     /gene="hxsD"
                     /locus_tag="ACHJ1G_003183"
     CDS             complement(6547..6819)
                     /gene="hxsD"
                     /locus_tag="ACHJ1G_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HFO4764986.1"
                     /translation="MSERVFEKELYSEWVIRNSLYWMTPLTRWKLHEDVSHWIISFEN
                     DNPKFLYEFDRLLNDYSLREKLQHKTGALRDSIVHKVLRSVDERLS"
     gene            7072..7332
                     /locus_tag="ACHJ1G_003184"
     CDS             7072..7332
                     /locus_tag="ACHJ1G_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4764987.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGAHSSEGIPWHELPASHKGNNQMSLPLPQN"
     gene            7523..7663
                     /locus_tag="ACHJ1G_003185"
     CDS             7523..7663
                     /locus_tag="ACHJ1G_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4764988.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(7969..8268)
                     /gene="ihfA"
                     /locus_tag="ACHJ1G_003186"
     CDS             complement(7969..8268)
                     /gene="ihfA"
                     /locus_tag="ACHJ1G_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4764989.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8273..10660)
                     /gene="pheT"
                     /locus_tag="ACHJ1G_003187"
     CDS             complement(8273..10660)
                     /gene="pheT"
                     /locus_tag="ACHJ1G_003187"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4764990.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(10675..11658)
                     /gene="pheS"
                     /locus_tag="ACHJ1G_003188"
     CDS             complement(10675..11658)
                     /gene="pheS"
                     /locus_tag="ACHJ1G_003188"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4764991.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(11868..11992)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(11942..11986)
                     /gene="pheM"
                     /locus_tag="ACHJ1G_003189"
     CDS             complement(11942..11986)
                     /gene="pheM"
                     /locus_tag="ACHJ1G_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4764992.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(12109..12465)
                     /gene="rplT"
                     /locus_tag="ACHJ1G_003190"
     CDS             complement(12109..12465)
                     /gene="rplT"
                     /locus_tag="ACHJ1G_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4764993.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(12518..12715)
                     /gene="rpmI"
                     /locus_tag="ACHJ1G_003191"
     CDS             complement(12518..12715)
                     /gene="rpmI"
                     /locus_tag="ACHJ1G_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4764994.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(12812..13354)
                     /gene="infC"
                     /locus_tag="ACHJ1G_003192"
     CDS             complement(12812..13354)
                     /gene="infC"
                     /locus_tag="ACHJ1G_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4764995.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(13358..15286)
                     /gene="thrS"
                     /locus_tag="ACHJ1G_003193"
     CDS             complement(13358..15286)
                     /gene="thrS"
                     /locus_tag="ACHJ1G_003193"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4764996.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            15810..17709
                     /gene="espL1"
                     /locus_tag="ACHJ1G_003194"
                     /pseudo
     CDS             15810..17709
                     /gene="espL1"
                     /locus_tag="ACHJ1G_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            17881..17988
                     /gene="yniD"
                     /locus_tag="ACHJ1G_003195"
     CDS             17881..17988
                     /gene="yniD"
                     /locus_tag="ACHJ1G_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO4764997.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(18041..18799)
                     /gene="ydiY"
                     /locus_tag="ACHJ1G_003196"
     CDS             complement(18041..18799)
                     /gene="ydiY"
                     /locus_tag="ACHJ1G_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4764998.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         5046 a         4785 c         4229 g         4869 t
ORIGIN      
        1 agtcgcggtg atgtcacgcg tgacagcagc gtaaacatgg attccgcttt cagattcagc
       61 acatcagaca gcgacaaatc tcgcagagat ccagcctgct caatagctcc ggtgatctcc
      121 acatacgtga ttttttcgcc gcctcgctca attggttggg taagttttac gccacgctca
      181 ctggtttctt tcacagtgtc agcaacgacc gtgttttcgg tatcgatgtt tttcgtctct
      241 ttcatcagga aactcctttc agtcagaggc gacgcactgc gccgcctgca tattacttat
      301 cagccaagcc cgagcgcgga acggatgcga tcgggcacaa tgtccttgcc gtccttccgg
      361 taaatgaagt tcagcaggtc aatctcccac aacgggcgat cgttaacact cagcttgtag
      421 taggtgtttt taatggcgta agtgtgtgat gtggcttcgc cctgtttggc ttcccccata
      481 tcaatttccg tcacacgtcc gcgcatttcg acttcataca ggtcgctttc tgcatcggtg
      541 taatattcac ccgcaaaacg cagcagcgtg ccgtcaatcg tgccgccata cttaaggaac
      601 agctcacgaa ctgcgccccc catgacaaag ctcgcatcaa gcgcggagtc gtccagaccg
      661 agatcaatac ttaccgcacc catcatgcca ccaccccggt agctgtcggt tttgcgcgtc
      721 agcttaggca gagtgacgga cgtcacctta cccacttcgt tttcaccatc cacaaacagc
      781 gtaaaaaagc gaagatgttt tggcacagcc atcaggcacc tcccagcacc gcaaatgcgg
      841 gaccaaagaa ttcatcagta aacgtctggt aaagctccat gtcttccagt ggcggaacgg
      901 gcgtatattt gtagcgaata cgcacacgcc cctgacgtaa atccgtggtg ccgttatcca
      961 ccacgtcata ccagcacgac gccccaatca gtttcccggc agtaaccagt gaatccagtt
     1021 ttgccctgat ggcactgata acatctttca cgttcgcagg cgtcagtgga ctgtcgatgg
     1081 tttcaaactg cgcttccgca attgaatcag ccagcacctg tgcggttcgg gtatacacct
     1141 caaagatgta ggcgttcgtt tccggtgtgc ggttgcccca gaagcggaac ccgttgcgac
     1201 gaataatggt cgtgatttct ttgttgttga ggctgttggc atcactgtct tcggcctgca
     1261 acgaccagaa cacatgcctc gacatcccca gcacattctt aaccggaacg ttggagagtg
     1321 atttgtgcca tccctgctca tggtcaatgt acgcacgaag gccgcacgca taggcaggcg
     1381 cggggaacgt ttcgtttttg ccacttttcg ggttgtaggc gatgaagtcc ggccataaga
     1441 gcatcacctc acgttcgttg aatttctggc ggtaggtaat cgcatcagcc atcgtgtcac
     1501 agccatgaca tgaggcatac acaaacgcgc gcagtttacc cgcaatcacg cacagggatt
     1561 ttgtcaccgc ctccgtatcc agctccggcg cggccagaat acgcggacgg tatccgatgc
     1621 tttcatcctg ctctgcaaca agcagcgcat acatccccgt atagctgccg tcagattcag
     1681 aaccaccgat aaccagttga tcctgcgtct ttccgtcttc ttctttgtgt tcagccacgc
     1741 gaacgacgat cacctttgtg ctcacctggt ctgcaatggc cttaagcgca cgataaagcg
     1801 tccccgttgt cccgcatttt cccagcacgt cattgacgcg ggtcagcagt gtgggcttgt
     1861 tcagcgggaa cagctccgcg tccgcatcat ccgccgttgc cacgataccg ataacactgg
     1921 aatcaacatc attaatcgct gttaccaggt cggtactttc cgtaacacgg gcaccatgaa
     1981 aacgagtttc actcatagct tcagcccctt gtatccgtta aatgattcgg caacaatcat
     2041 cacccaccac gcgcgtaatc tcactcctgc gccgttctcc cgccacggcg acaacaaaaa
     2101 gcagtaaccc cctccgcacg cacatgcgac catgccgcac agggagggaa cagatgaccg
     2161 acaccaccat gcaattgctc agtcagggca cagaccccgt gaaaatgccg gattttgata
     2221 ttctcgcgga gggtaaaacg ctgtcaggcg tggcagagcg cctgatgagc ctgtcactga
     2281 ccgacaaccg gggatttgaa gcggaccagc tcaccatcac gctggatgat gcggatggtc
     2341 agttgcagct accgccacgg ggcgcgcgcc tgacggttct cattggctgg aaaggagaac
     2401 cgctgacaga aaaaggcact tacattgttg atgaaatcgc tcacgaagga ccgccggaca
     2461 ggctgactgt ttcagccaga agcgcagatt ttcgggatga atttaacgtt aaacgtgagg
     2521 tgtcctggca tgatgtgacc gttgagcgtg tggtatccgc catcgctcat cggtatggtc
     2581 tgaaaccgca aatcagcgaa atgctgatgg atatcgaaat cgaccacgcc gaccagaccg
     2641 aagaaagcga catgtccttc cttacgcgca tggcggaaat gctgggcgca atcaccacag
     2701 taaaaagcgg caatctgtta ttcatcatgc caggtggtgg cgtgaacgca cagggccagc
     2761 cgttgccctc gttcgccatt acacgcagca gcggcgatcg ccatcagttc cgtattgctg
     2821 accgcgaggc gtatacgggg gtacgcgcct actggcttga tcttaattac gggaaaaaga
     2881 aaaaagtcag cgtgaaacgc cgtaaaccgc caaaacccaa aaaggagaaa agcagcagcc
     2941 gtgaaggtga ttatatggaa ggcgcggaag gcaatgtgtt tgtgttacgc aagacttatc
     3001 agaacgagca ggcagcaaga cgcgcagcgg cggcaaagtg gcagcagcta caacgcggag
     3061 ccgcagcgtt ctccatcaca ctggcacgtg gacgcgcaga actctacccc gaaatgcatg
     3121 gcacggtaac aggctttaaa agcgagattg ataatcagga ctggattatt gcgaaagccg
     3181 agcacaccat tgataacagc ggctttacca cgcagcttga gcttgaggca aaaatcccgg
     3241 aatggatagc agaatgagac tcttaaatgt attagtccag acacgtacta gcgggctaaa
     3301 cggcacagtc ttatctgacc atctgcccct aacacagagc agacggtcct tgatcttacc
     3361 cactaataat ggacgcgcga ctaatataat ttattgcttt ttatttatac ctcactgaga
     3421 tttagcgagc taatatattc aaagaattta acaattgagt gtcgagtgtt tcagttgcag
     3481 gaatcggtaa tccattcgca atgcggtaat ttttcacaga cagccttgtg gatggcccca
     3541 ttatgccgtc aatatttccg ttataaaatc ccctgtccag caatgcgaac tgaactcgca
     3601 taatcaatcg cttacgtttc tctgcatcag aagcaagccc attactcgta cggttaacct
     3661 ctgaagttcc aacagaactc gtgttagtgg ttctattatt atcgttagaa cgaagtgcac
     3721 gcaccgagga agaggacgat gaggaacttg cacctgaact tgaagaacca gatgaacgtg
     3781 gtgagctgta tgtttttgga taataggggg tacttccacc atagtatcca ccacctgatg
     3841 aggatctatg agagctatgg ctcctatgag agctatggct tcgatgccct gcgatataaa
     3901 atgggacttc cgtgttaagt ggagctatca ctaaatcatg ctcattaaga gtcataccag
     3961 gtaaatcact cgctcctgta gatgaatcgc ttgcccatac agaattgttg agagccaaaa
     4021 atcccggaag tagagcagca aaattaaact ttttcataaa attggtctcg gtgattgatg
     4081 aaaacggcct tttgaggaac tgaaatagcc agcacaggaa ggcttctgag tacattcatc
     4141 acattctttt ggatagtaat ttttccaatc agagatcgac tgaacagcaa gctccctagc
     4201 cctttcagga agataacaca aaggataatt aaaaattgtc agaggtatgc ctgacctgta
     4261 tgcagcgtct atggcagaga ttattttctc actataacta ctgtgatcaa tgaagatggt
     4321 ggaccagttt tttcgtgccc aaccgatagg ctccaacccc ataagggaaa tctgattgat
     4381 gttggagaac acacggccag cgaattctac aatatcatcc aattcggtat agttagccag
     4441 tgtaggaata actctaagtt cgatattaat ccccaagttt cctgcattga ttaacccttt
     4501 aaccgtttca ttaaatgcgc catcactccc taccagatga tcatgcacaa gtggtcttga
     4561 tgagtagagc gggataccaa aggtgatttt gatctttttg cttcgctttg ccatttcctg
     4621 tgtaaagttg atatcagcaa atttgcgtcc gtttgttaaa acatgtaaag cagtatctgg
     4681 tgaattctcg atgataaaat caatgaagtg aaggaagtca tccccatata gcaaaggttc
     4741 accaccgctg accccaacaa ctccgttcaa gccaaatgaa gctatagcaa gagctgattg
     4801 ggtaagtagc cagtcatcat ttattttctt tggtggctgc gaacaaaaca gacacaagtt
     4861 attgcagcgt tccgtaacta agacagtatt atgattggct ttgcgtgaca gaataacgcg
     4921 tatcatattg ccattgttga ttatcccaat atctccatct tcaattgact caaataattc
     4981 agtgctaata acagagaaat caaagcatgg tggaatggca tcattattgc cttcagcaac
     5041 tactagcaaa ttgggcaaaa agaataaagg gttcactggt ttttgtttgc ataaccgata
     5101 gaaccccgtg ggcacgttct ctgtagaagc aaaatgaaag atatcgttcc tgataacctc
     5161 agacatatgc ccatcctttt agcatctcag ccttcgaacc accctctgag atgcagttca
     5221 tcagatacct gaacatcccc ttgtgatatt gacaaaacgt tgaacgactc ctatcaccga
     5281 caggctcacc ctgtacactt atgttctggc atggatctga accacaaaat ggttgatatg
     5341 cacatgtatc acaacctggc agagcaaaat tgaacgatga actcaatact gcgttatagt
     5401 aaggactatc agaaaatgat agcgtcttaa cttcacccgc actgaactcc gtttcaggat
     5461 tcaccttctg caacatacgg ctctcatcgc taccatatac tcgaccgtca taattgaaaa
     5521 gaatactgtt aagcacgact ccacttggag actttaaatc cgcataacca ctaaaaccgg
     5581 gattgaagat ccttttcaga tgaatggagg ccgaatgttc cacaaccctt atcccttcat
     5641 tattaatctt aaggatttca tctatcaacg aggcatagaa gctgaagtat tcgtccatag
     5701 aaaaggtgaa agaagctttt tgtgcaaacc catagggact aacaggtcgg ataaacatgt
     5761 ccttgagacc aagagacaag tgagcctgaa caatcgattc aggacgtttg ataagctctt
     5821 tagtaactgt agtaaccgtt gcaacgcggc cagcacctaa agagtgtttg atagcctcta
     5881 cccctgacac tgttctactg tacgcaagcc cacgaccaag aattcgatta ctgttgtgaa
     5941 caacttcctc accatcaagc gacacagaga acgttatgtt ccggtctctc gaccattcaa
     6001 tcatatcctc attcaagacg gacagacttg atgccaccac catctcaaaa ttggttcttc
     6061 ccagcgtttc agcacattga tcatagatgc tctgaattaa atcaaaccgc aagagaggct
     6121 ccccaccctg tatctctatt ttgtaaggtg gcgttgatag ttttttaata gccgaaataa
     6181 tatcggggat gagctcagga tttaggtcgt aacctgaggc attaacagaa gctctactaa
     6241 cctggcaata cttgcatgta tggtcacaac gtaatgtggg tacaatcatg aaaattggcc
     6301 tgacagccag ttcattcatc aatcgttttg caaaagcaga acttaaagca tagggagtta
     6361 tagcagatga gctttcactg gttatgaaaa gcttactttc tagcacgttt gattgctccg
     6421 aactaattcg ctcatcagaa agatcaataa ggtcttgctc accaatgaaa tgatgaaaac
     6481 ctgcaaggtt gcttataaac acctgtccat taggcaatct gtcaaaatta aatggcatca
     6541 attccattat gaaagcctct catcaacact gcgaagcact ttatggacaa tagagtctct
     6601 caacgcccct gttttatgct gtagcttttc tcgcaaagag taatcattaa gcagcctatc
     6661 gaattcataa aggaatttgg gattatcgtt ttcaaaagag attatccagt gggaaacgtc
     6721 ttcatggagt ttccatcgag taagcggagt catccaatat agactattgc gtataaccca
     6781 ttcagaatat agttctttct caaacactct ttcgctcatt gcaattatct tccaatacaa
     6841 atattcatga tcagcaacca ctttaaagcg gatttttcct atggcaacaa taatataaca
     6901 tatccataga tatgttacag atgccttgtt tcccgttgac taatacatca caacgttagt
     6961 aatggctatc acaattgtga acttccactt ttcacacaag acaagctgtt aattggaatc
     7021 tgtatcagta caacttagaa tagaggaagc accacagtaa gggaggtcgc tatgttccgt
     7081 tgtccacttt gtggcgcatc tgcccgtatc cgcactagtc gtccggaaaa tgattcaaac
     7141 actgtgcggc aaaagtatta ccaatgtaac aacctagaat gcggcgtatg cttctcaaca
     7201 ctggaagctt tccataaatt cacatcgaaa cacgcctccg gcgctcactc ttcagaaggt
     7261 atcccgtggc atgaactgcc agcttcacac aaaggaaaca atcagatgag tttgccctta
     7321 cctcaaaatt aacaggcaga attgccggag taacaaaaaa gagatagatt acgcgcgggt
     7381 gcctttcggc tgatggtcgg agggaatacc tgaaggccag atgtggaaag gccccggaaa
     7441 acatttctgt ttaaccgagg ccctaaccgc attaccttga caagtgaaag gttagcgcct
     7501 ctccggaaaa ggagcaagtg ctatgtcgca aaaatcgctt acggccatca cgttctgcgt
     7561 gacggcaatc ctcatcatct ggatgttgca cggttcgctg tgcgaaatac ggatgagctt
     7621 ctggggagcg gagtttgcgg cgttcttaca gtgtaagcag taaggaaacc gcgacggggg
     7681 agcaatcccc cgtcaatcgg ttgccagggt aaggtcgata aggcacccta tctcacagac
     7741 atgaacaaca aacccgcagc gtaaaaactg cgggttttct ttttggtgtc cttactgact
     7801 tgaaacggtg atcagactat aagacaaagc ccacaatgta gcgggcttac tcccctctcc
     7861 ctccactgaa tgtggacaca tggtggaaca aagagatata aaacattata aatcaatcgg
     7921 ttattgaaag aaaaaaggcc acagagcggc ctttttagtt agatcagatt actcgtcttt
     7981 gggcgaagcg ttttcgaccc ggctttttaa cttctgcccg ggtctgaagg tcaccacgcg
     8041 ccgtgctgta atgggaatat cctcgcccgt tttcgggtta cgtcccgggc gttgattctt
     8101 atcacgcaga tcgaagttac caaaaccaga gagtttcacc tgttcgccgt tttccagagc
     8161 gcgacggatc tcttcgaaaa acagttcaac cagttctttg gcatcccgct tgctaagccc
     8221 aagcttatca aacagatatt ctgacatttc agcttttgta agcgccatag gttcaatccc
     8281 tcaatgatgc ctggaatcgc tcttttaatg cctctacaca tttggcgacg gtagcggcaa
     8341 tctcctcttc ttcgagtgta cggctggtat cttgcaggat caggcttatg gcgaggctct
     8401 tatacccctc cgcaacaccc ttaccgcggt acacgtcaaa taagtttacg ccaactacct
     8461 gatttacgcc aactttctta cattcggata aaatatccgc tgcgggaacg ttttctgcga
     8521 ccaccaccgc gatgtcacga cggttcgccg ggaagcgaga aatctcgcgc gcctgaggca
     8581 ccacgcggtc tgcgagcttg ttccactcca gttcgaacac cagagtgcga ccgttaagat
     8641 ccagtttacg ttccagttca ggatgaacaa ccccaacaaa accaatacgt tcacctttca
     8701 gataaatcgc tgcggattgc cccggatgca gtgccggatt cgcttctgca cggaactcaa
     8761 cctcattcag tttaccggtc aggtcgagaa cggattcaag atcgcctttc aaatcataga
     8821 aatcaacggt ctcttttgcc aggttccagt gctcttcgta acggttaccg caaatcacac
     8881 cggctaacat cagatcctga cgaatgccca acggtgcctg agtatctggt acgaaacgca
     8941 gaccgctttc gaaaatgcgc acacggttct gctgacggtt ctggttgtac accacggttg
     9001 ccagcaggcc ggtccacaga gaaagacgca ttgctgacat ttcaacagag atcgggcttg
     9061 gcagcagtaa ggcttcaacg cctggatgga tcatctgctg cactttcgga tcaacgaagc
     9121 tgtaggtgat cacttcctga tagcctttgt cgttgagcag cgttttcacg cgcttgagcg
     9181 acaggtcagc ttcacggtga gtacccataa tcaggcttgc ctgtaccggc tcatccggga
     9241 tgttgttgta gccgtaaaca cgcgcgactt cttcgaccag atcttcttca atctccatat
     9301 cgaaacgcca gctcggcgca actgcctgcc actcgtcttt gccttcggtc acttcgcagc
     9361 cgagacgacg cagaatgtca gttacctgct catccgcaat atgatggccg atcaggcgat
     9421 ccagtttgct acgacgtaaa gtgatggttg cacgcttcgg cagcgttgct tcgttggtga
     9481 tatcaattac cggaccagcc tcaccaccgc agatgtcgat cagcagacgg gtcgcacgtt
     9541 ccatcgcttt gtgctgcagt gccggatcaa cgccacgctc ataacggtga gacgcatcag
     9601 tatgcaggcc atgacgacga gcacgaccgg tgatagacag cgggctgaag aaagcgcatt
     9661 ccagcaagac gttttgcgtt tcgtcattca cgccagagtg ttcgccacca aagatgccgc
     9721 ccatcgccag cgccttgttg tggtcggcga tgaccagagt gtcagcattc agcttcgctt
     9781 cagtgccatc aagcagtacc aacgtttcgc cctctttcgc catccgcacc acaatgccgc
     9841 cttcaatgcg atctttatcg aaagcgtgca tcggctggcc cagttcgagc agtacatagt
     9901 tggtgacgtc aacaactgca tcgatagaac ggatcccgca acgacgcagt ttttctttca
     9961 tccacagcgg agttggcgct ttaacgttaa tgccttttac cacacggcca agataacgcg
    10021 ggcaggcttc cggcgcttcg actgtaatcg gcagcgtgtc gtcgatggtc gcaccaaccg
    10081 gaacgatttc cggttcaacc agcggcagct gattcagcac ggcaacgtca cgcgcaacac
    10141 caatgatacc taaacagtcg gcacggtttg gcgtcacgct gatttcgatg gtgttgtcat
    10201 caagtttcag gtattcacgg atgtcggtgc caatcggcgc atccgcaggc agctcgataa
    10261 tgccgctatg atcgtcagaa attcccagct cagaaaagga gcacagcatc ccttcagacg
    10321 gttcaccacg cagtttcgcc gctttaattt tgaaatcacc cggcagaaca gcaccaatgg
    10381 tagctaccgc cacacgcagg ccctgacggc agtttggcgc accgcagacg atgtccagca
    10441 ggcgatcgcc gccaacattc acttttgtca cacgcagttt gtcagcgttc ggatgctgcg
    10501 cacactcaac cacttcacca acgaccacgc cgtggaagct accggcaacc ggttctacac
    10561 cgtcaacttc caggcccgcc atagtgattt gattcgccag cgcatcgcta tcaatcgccg
    10621 ggttcaccca ttcgcgtaac cacagttcac tgaatttcat aatctattcc tgccttattt
    10681 aaactgtttg aggaaacgca gatcattttc gaagaatgaa cgcaggtcgg tgacgccgta
    10741 acgcaacata gtcagacgct ccatccccat cccgaaggcg aaaccagagt aaacttccgg
    10801 gtcgatgcca acgttacgca gcacgttcgg atgcaccatc ccgcaaccca gcacttccag
    10861 ccatttaccg tttttaccca tgacgtcaac ttctgcagaa ggctcggtga acgggaagta
    10921 ggaaggacgg aagcgaatct gcaaatcttc ctcaaagaag ttacgcagga aatcgtgcag
    10981 agtgcctttc aggttggtaa agctgatgtt ggtatcaaca atcagacctt ccatctgatg
    11041 gaacatcggc gtgtgagtct ggtcgtagtc gttacgataa acgcggccag gcgcaatgat
    11101 acgaatcggt ggctgctggg ctttcatggt gcggatctgt acgccagagg tctgggtacg
    11161 cagcaggcgg gtagtgtcaa accagaaagt gtcgtggtca gcgcgcgccg ggtggtgacc
    11221 aggaatgttc agagcatcga agttatgata atcgtcttcg atttccggcc cggttgccac
    11281 ggtaaagcca agctcaccga agaaactttc gatacggtcg atagtacggg taaccggatg
    11341 cagaccgccg ttttcaatgc gacgacctgg cagagagaca tcaatcgttt ccgccgccag
    11401 acgcgcattc agtgcagcgc tttccagttc cgctttacgc gcattcagcg cctgctgaac
    11461 ctgctctttc gcttcgttga taaccgcacc agctgccgga cgctcttctg gcggcagctc
    11521 acgcagggtc gtcatctgaa gggttaagtg ccctttttta cccaaatatt cgacgcgcac
    11581 attatctaac gcggcaacat ctgacgcctg gctaatggcc gccttcgcac tggcaaccag
    11641 ttctgcgaga tgtgacatgg ttttcctcat tgtgtcagtg gtgacactgg ttcgttggac
    11701 ttagagccta tcccatcagg ctattttact tgccattttg gtcccgggca gtgctcgaaa
    11761 ttctcacgta cttaaatacg ctccggtttc tccgcgctgg ccgtgtccag actggctgcg
    11821 acaattacac ctgatgagac aggcttttta tttttcaaaa cgcgcataca aaaaaagcct
    11881 ccactgggag gctttcaggc gctgttttcc gtttctcttc tcacgcgcta gcctcctgga
    11941 ttcaggtgct aaagtaaaaa aagaagcgga aaatagcagc attcattgct tgcgttacct
    12001 tttggtactc ttcaaaagac ctttattgaa aaggctacgg cgataaaagt caatgttttg
    12061 atggcgttga aacgaaaaga gggagactag ctccctcttt caactggctt atgccagagc
    12121 tgctttcgct ttttcaacca gagcggtgaa cgctactttg tcgaatactg cgatatcagc
    12181 caggatctta cggtcgattt caacagaggc ttttttcagg ccattgatga atttgctgta
    12241 agaaataccg ttctgacgtg ctgctgcgtt gatacgcgca atccacagtt gacggaactg
    12301 acgcttacgt tgacgacggt cacggtaagc atactgacca gctttgataa cagcctggaa
    12361 ggcaacgcgg tatacgcgag aacgcgcacc gtagtagcct ttagcttgtt tcaaaatttt
    12421 cttgtgacgt gcgcgtgcaa taacaccacg ttttacgcga gccatatgtg ctctcctgta
    12481 tctatattct aattaaaaag ttaaaaacgt taacggctta tgcgtacggc aggcacgcga
    12541 ttaccaggcc cagatcgcct ttggaaacca tggctttcgg acgcaggtga cgtttacgtt
    12601 tggtcgcttt tttggtcaga atgtgacgca ggttagcgtg cttgtgctta aaaccacctt
    12661 taccggtttt tttgaagcgc ttagcagcac cgcgtacggt cttaattttt ggcattttaa
    12721 taacttccac ttcgcattgt taataaacga aacaaaggcg aacaaagcct gtgaagcccg
    12781 aaggctccac agacagtgct acttgaaggc cttactgttt cttcttagga gcgagcacca
    12841 tgatcatctg acggccttcg atcttcgttg ggaaggattc gaccactgcc agttcttgca
    12901 aatcgtcttt cacgcgatta agcacttcca taccgatttg ctggtgcgcc atctcacgac
    12961 cgcggaaacg cagcgtgatt ttggctttat caccctcttc gagaaagcga atcaggctgc
    13021 ggagttttac ctgatagtcg ccttcatctg taccaggacg gaatttaatt tccttaacct
    13081 ggataacttt ttgctttttc ttctgttcct tagaagactt gctcttttca tagaggaatt
    13141 tgccgtaatc cattatacga caaaccggcg gctcggcgtt agggctgatc tcgactaagt
    13201 ctactccggc ttcttctgct ttctccagag cttctctcag actcacaata ccaagctgct
    13261 cgccttccag acctgttaag cgaacttcct gggcgcgaat ttcgccattg atacggttag
    13321 ggcgcgccgt ttgaactcgt tttccgcctt taatacctta ttcctccaat tgtttaagac
    13381 tgcggctgcg aatctcttgt tgcagcttct cgatcacttc atttacgtcc atgcttccca
    13441 ggtctttacc acggcgggtg cgaacggcaa ctttgcctga ttccacctct ttatcaccac
    13501 agaccagcat atatgggacg cgacgcaaag tgtgctcgcg gattttaaag ccaatcttct
    13561 catttctcaa gtctgcttta acacgaatgc ccgcatttga tagtttttgc gtcaattcgt
    13621 taacgtattc agactgtgaa tcggtaatat tcatgataac aacctgaact ggcgcaagcc
    13681 aggtcgggaa gaaaccagcg aattcttcgg tcaggatacc gatgaaacgt tccatcgatc
    13741 ccagaattgc gcggtgaatc attaccggta ctttacgttc attgtcttcg cctacataag
    13801 aagcgctcag acgagaaggc aaagagaagt ccagctgtac tgtaccgcac tgccatgcac
    13861 gatcgaggca gtcatacagg gtaaattcaa ttttcggacc gtagaaagcg ccttcaccca
    13921 gttgatattc aaacgggatg ttgttttctt ccagcgcaac cgccaggtcc gcctcagcac
    13981 gatcccacat ttcatcactg ccaatacgtt tttcaggacg agtggagagt ttgacgacga
    14041 tcttctcgaa gccaaaagtg ctgtacatat catagactaa acggatacat ccgttaactt
    14101 catcgcgaat ttgttcttca gtacagaaga tatgcgcgtc atcctgggta aatccacgca
    14161 cgcgcatcag gccatgcagc gaacctgacg gctcattacg gtggcagcta ccaaactcgg
    14221 ccatacgcag cggcagatcg cgataagact tcagcccctg gttgaaaatt tgtacgtgac
    14281 ccgggcagtt catcggctta atgcagtatt cacggttctc agaagacgtg gtgaacattg
    14341 catctttgta gttgtcccag tgaccggttt tttcccacag gacacggtcc atcatgaacg
    14401 gacctttaac ttcctgatac tggtactctt tcagtttaga acgaacaaac acttctagtt
    14461 cacggaagat ggtccagccg tcgttgtgcc agaataccat acccggcgct tcttcctgca
    14521 tatggtacag gtcgagctgt ttaccgattt tacggtggtc gcgtttcgcg gcttcttcca
    14581 ggcgctgcag gtaagcgtta agcgcttttt tgtctgccca cgccgtaccg taaatacgtt
    14641 gcaacatttt gttgttgctg tcgccacgcc agtaagcccc tgccgttttc attagtttga
    14701 aatgatggca gaaacgcatg ttcggtacgt gcggaccgcg gcacatatcg acatattctt
    14761 catggaagta cagacctggc ttgtcatcat gggcgatgtt ttcgtcaaga atggagactt
    14821 tgtagctctc cccacggttg gcgaaagttt cacgcgcttc gtgccagctg actttcttct
    14881 taatgacgtc gtagtttttc tcagcaagct catgcatccg cttctcgagt gcttcgacat
    14941 cttcctgggt taacgtgcgg tcaagatcaa cgtcgtaata aaaaccgttg tcaataaccg
    15001 ggccgattgc cattttggta tgcggccaaa gttgtttaat cgcgtgccct aacaggtgcg
    15061 cacaggagtg acgaatgatc tccagaccgt cttcgtcttt ggcggtaatg atggacagtt
    15121 gtgcgtcgtt ttcaatcaga tcgcaagcat caaccagttc gccattaacg cgccctgcga
    15181 tacaggcttt cgccagacct ggaccaatgt ccagcgcaac atccatgggg cttacagcgt
    15241 ggtcgtaatg gcgttggctg ccatcaggaa gagttataac aggcatttta tatccttatt
    15301 tgcagtggtg acccacacga aagatcacat acaaagaaaa atttgtttat taacagttca
    15361 ttgcgaaacc atctagccaa caaatgctaa attggttcgc aatcaggtac acaaatgaaa
    15421 gacattaacc tctcaccttc cacccggttg agcgatgtta acactaaaaa aagggagatt
    15481 gtacctttcc gtttcacatt gattcgattc gcaattcgtt tgtagtgagt acaccagcaa
    15541 aacgcttaaa gaaccactga taacatcacg gtgcgtctga aaggtttact gcgagcggat
    15601 ttacagcagc ataaactgaa tccagcaaag ttcgttgcgt gatttggcgg tagtatttta
    15661 aacgctcatg agattaatga cgaagtggtc atatcacaat gataaaagtg acacaattct
    15721 tataacaatt tttcgtgcac atttcgttct ggcgataata attaatcatc atcctcaaaa
    15781 cctccagata tatataaggc gaatagatta tgagtcaaaa cgatatcatt atcagaactc
    15841 attataagtc tcctcataga ttgcacatcg atagcgacat accaacacct tcatcagagc
    15901 ctattaataa atttgcgcgc cagctcatca ccctacttga tacctctgac ttaagttcga
    15961 tgctgtcata ctgtgttact caggaattta ccgcaaactg tcgaaaaata tcacaaaatt
    16021 gttattccac tgcccttttt accattaacc ttgccacttc acccatccat gcagaaaata
    16081 tactcattac attacactat aaaaaagata tcatttcctt attactggaa accacgccta
    16141 ttaaagctaa ccatttgcga agcatactgg attatattga acaggaacag ttaactgctg
    16201 aagatcgtaa ccattgtatg aaactgtcta aaaaaatcca tagagaaaaa actatacacc
    16261 caacagtaaa tctcaatggt agtgcatgtt ttttgcaatc tccttctgac gctatttttt
    16321 gtcgccatct gtcattgcaa tacgcccttg attcattgag aaatggaaaa ggcaaagtca
    16381 acctgattaa acattactcc tccgttgaat ccatacagca gcatgtcccc ttagtccggg
    16441 acgcggagtt cagagcatta cttcgccatc ctcctgcagg gagtcgcgtt atcgcgagta
    16501 aggattttgg cttcgcttta gattttttct tttgtcgaat gatggcaaac aatatcagtc
    16561 atatgtccgc gattttatat atagacaatc atactttgtc agtaaggcta cgaataaagc
    16621 agtcagtgta tggacaatta aactatgttg tgtccgttta cgacccgaac gataccaacg
    16681 ttgccgtcag agacacccac aggacagcac ggggctttct ttcgcttgat aagttcatca
    16741 gttcaggtcc cgatgctcag acctgggctg ataggtatgt tcgcaactgt gcaattgcta
    16801 ttctgcccct attacctgtg ggagttccag gggctatttt cgcgggtatt gcatcacgaa
    16861 tgccatttgc ccctatacat ccatcggcaa tgttgttaat aatggctaca ggccagactc
    16921 aacagcttat tacattattc aaacagttgc ccatactccc tgaaaaagaa atcattgaaa
    16981 taataactgc gcagaatagc gttggtacac ctgctttatt tttggctatg atgaacggac
    17041 atactgacaa cgtgaaaata tttatgcaag aaattcagtc actggtagat aatcacatca
    17101 ttcatgaaga taacctggtt aaattactgc aaactaaaag tgctaacgaa acacctggac
    17161 tctatatctc catgttgtat ggattcgatg aaataatcga tatctttctg aatgcattaa
    17221 ccactccaat agcacaagaa cttttaaaca aaaaaatggt gatggatatt ttagccatga
    17281 aaacacgtga tggtgaacca gggttatttg ctgcaatgga aaataatcac cctttgtgtg
    17341 tcacacggtt cctctctaaa gtttatggga tcgccgttaa atataaactc agcaaaatca
    17401 acatcatgga cttattaaaa ggcgcgacag cacagggaac ccctgcatta tacatcgcca
    17461 tgagcaaggg taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa
    17521 aaaaaacatt cttttagtca acatcagtta tttacactat tggccgctaa aaatcatgac
    17581 aacatgtcag ctgttcatat agccattcat cataagcatt ataaaactgt agaaacatat
    17641 tatgctgcta taaatgtcat cagccaaagc ctgagtttta gtgctgatga aataaagacg
    17701 tatttataac cagctattta cgatatatat cgatacataa ttttattttt ttcataaaac
    17761 gcagaaacag tgaccaggtg tacaacgcca cccaacggct gtaataacaa aaaatccctt
    17821 gatgcccgtc ccttttgtta cactccgtta tcacgcacaa gagatatgca ggacactggt
    17881 atgccgacta aacgctttga taaaaaacac tggaagatgg tggtggtgct actggcaatc
    17941 tgtggcgcta tgttgttgct acgttgggca gcaatgattt ggggctgagc agtgacaaac
    18001 gaagacagcc agaccgcata acacggtccg gcagataatt ttacatgctg tagcccagcg
    18061 ataaggttgt ccgacggtcg gtatgttccg gtgcagactc tggaggttca gagttccatg
    18121 tgacgttgta agccaccttc agtccaaaat gttcattaat agcaacattt aatgcgcttt
    18181 cggagttcag cgttgtgtct tccgcgccaa agaccgaaac accctgcgta aatttagcgt
    18241 tgtcagtcaa ctgccatgca taagccccgg aggcatagcc cagcggctgg gtttcactgg
    18301 cattgtcggt atatttgtcg taacgcacac ctggaccgaa ttcaaagcgg aaactgtgta
    18361 ccgggccatt caaaaactga tgaccataac ccgcggtcaa cacatcgcgc tctcgatagc
    18421 cgttataacg gtctgtcagc cagcttgcct gaccaaataa atagtcataa tcagttaaat
    18481 taaaacggct acgcccgccc gccgcatatt tttctgaaga acgctcatca ttagaagaag
    18541 tattactggc gttcccccac agcgaccagg cagtggtttg tccataccag gtcatggtgg
    18601 tgtcagccgt aagtgaggag cttttcgtat tgcctgattg tgcaagatat cctgcgttca
    18661 gattaccttc gaaaggtttt ttagcgctgg caggatcgtc catgacagta aaaacggaat
    18721 catcggcagc tgcattcagt gacgcaaaca tgccccccgc caacataacg atggcaggaa
    18781 ctgtcttcaa aagcttcatt tatcaagagt ccgtacaaca aaaaaagaga ccatcgcggt
    18841 cccggaaact ttcttaagga tcaaagatta gcgtccctgg aaaggtaacg aattataaaa
    18901 aggcgcgaat aacttagcaa tgtattctt
//
LOCUS       DAZFDP010000090        18617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFDP010000090 DAZFDP010000000
VERSION     DAZFDP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..806
                     /gene="yieP"
                     /locus_tag="ACHJ1G_003197"
     CDS             114..806
                     /gene="yieP"
                     /locus_tag="ACHJ1G_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4764999.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            829..2256
                     /gene="mdtD"
                     /locus_tag="ACHJ1G_003198"
     CDS             829..2256
                     /gene="mdtD"
                     /locus_tag="ACHJ1G_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4765000.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2222..3214)
                     /gene="rbsR"
                     /locus_tag="ACHJ1G_003199"
     CDS             complement(2222..3214)
                     /gene="rbsR"
                     /locus_tag="ACHJ1G_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4765001.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3218..4147)
                     /gene="rbsK"
                     /locus_tag="ACHJ1G_003200"
     CDS             complement(3218..4147)
                     /gene="rbsK"
                     /locus_tag="ACHJ1G_003200"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4765002.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4273..5163)
                     /gene="rbsB"
                     /locus_tag="ACHJ1G_003201"
     CDS             complement(4273..5163)
                     /gene="rbsB"
                     /locus_tag="ACHJ1G_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4765003.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5188..6153)
                     /gene="rbsC"
                     /locus_tag="ACHJ1G_003202"
     CDS             complement(5188..6153)
                     /gene="rbsC"
                     /locus_tag="ACHJ1G_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4765004.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6158..7663)
                     /gene="rbsA"
                     /locus_tag="ACHJ1G_003203"
     CDS             complement(6158..7663)
                     /gene="rbsA"
                     /locus_tag="ACHJ1G_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4765005.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7671..8090)
                     /gene="rbsD"
                     /locus_tag="ACHJ1G_003204"
     CDS             complement(7671..8090)
                     /gene="rbsD"
                     /locus_tag="ACHJ1G_003204"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4765006.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8257..10125)
                     /gene="kup"
                     /locus_tag="ACHJ1G_003205"
     CDS             complement(8257..10125)
                     /gene="kup"
                     /locus_tag="ACHJ1G_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4765007.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10348..11844
                     /gene="ravA"
                     /locus_tag="ACHJ1G_003206"
     CDS             10348..11844
                     /gene="ravA"
                     /locus_tag="ACHJ1G_003206"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4765008.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11838..13289
                     /gene="viaA"
                     /locus_tag="ACHJ1G_003207"
     CDS             11838..13289
                     /gene="viaA"
                     /locus_tag="ACHJ1G_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4765009.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13294..14286)
                     /gene="asnA"
                     /locus_tag="ACHJ1G_003208"
     CDS             complement(13294..14286)
                     /gene="asnA"
                     /locus_tag="ACHJ1G_003208"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4765010.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14438..14896
                     /gene="asnC"
                     /locus_tag="ACHJ1G_003209"
     CDS             14438..14896
                     /gene="asnC"
                     /locus_tag="ACHJ1G_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4765011.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            14986..15429
                     /gene="mioC"
                     /locus_tag="ACHJ1G_003210"
     CDS             14986..15429
                     /gene="mioC"
                     /locus_tag="ACHJ1G_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4765012.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15807..17696
                     /gene="mnmG"
                     /locus_tag="ACHJ1G_003211"
     CDS             15807..17696
                     /gene="mnmG"
                     /locus_tag="ACHJ1G_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4765013.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17760..18383
                     /gene="rsmG"
                     /locus_tag="ACHJ1G_003212"
     CDS             17760..18383
                     /gene="rsmG"
                     /locus_tag="ACHJ1G_003212"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4765014.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4689 a         4836 c         4594 g         4498 t
ORIGIN      
        1 agcaaaagcc ccgttaatgt gccataaaac aagcaatttg ccgtttttgc aaccgtaatc
       61 acacttcctg gtggcatact aaggaggaat aaaaagaaaa aggaagatta gtaatgcctt
      121 taagcgcaca acagttagct gcacagaaaa acctttcgta tgttctggct gagaagctgg
      181 cgcaacgcat cttaaaaggt gaatatgaac ccggcaccat tttgcctggt gagattgagc
      241 tgggcgagca atttggagtg agtcgtacag cggtacgcga agcggtcaaa acgttaacgg
      301 caaaagggat ggttttaccg cgaccgcgaa ttggtacccg ggtcatgcca caatcaaact
      361 ggaattttct tgatcaggaa ttgcttacct ggtggatgac agaagagaac tttcatcagg
      421 tcatcgatca ctttctggtt atgcgtatct gtctggagcc gcaagcttgc ctactggctg
      481 caacggttgg caccgcggaa cagaaggcgc atctcaatac gttaatggcc gaaatggcgg
      541 cattaaaaga gaactttcgc cgtgaacgct ggatcgaagt cgatatggcc tggcatgagc
      601 atatctatga aatgagcgcc aatccgtttt tgacctcatt cgcctcgcta ttccattcgg
      661 tttatcacac ttacttcacg tcaattacca gcgacacagt gataaagctg gatctgcacc
      721 aggcgattgt cgatgcgatt gtccaaagcg atggcgacgc ggcatttaaa gcttgtcagg
      781 cgctgctacg ctcacctgat aagtgacaac ccgataatag gacattgaat gagcgataaa
      841 aagaagcgca gtatggcggg tttgccgtgg atcgcggcga tggccttctt catgcaggca
      901 cttgatgcca ctattctgaa taccgcctta cccgcaatcg ctcatagcct taatcgttct
      961 cctctcgcga tgcaatcagc catcatcagt tatacgctga cggtggcgat gcttattccg
     1021 gtaagcggat ggctagccga tcgcttcggt acgcgtcgca tttttaccct tgccgtgagt
     1081 ctgttcacat tgggttctct ggcctgcgca ctttctaatt cgctaccaca gctggttgtc
     1141 ttccgggtta ttcaggggat aggcggcgca atgatgatgc ctgttgctcg gctggcctta
     1201 ctgcgcgctt atcctcgtaa tgaacttctt ccagtattga attttgtcgc catgccgggt
     1261 ctggtggggc caattttagg ccccgttctt ggcggcgtgc tggtcacctg ggcaacctgg
     1321 cactggatat ttttaatcaa tatccccata ggtattgcgg gccttcttta cgcgcgcaaa
     1381 catatgccca atttcaccac cgcacgacgc agattcgata tcactggctt tttgctgttt
     1441 ggcctcagcc ttgttctctt ctcaagcgga atagagctat tcggggaaaa gattgtcgcc
     1501 agctggattg ccttgacggt aattgtcacc agcatcgggt tactgcttct ctatattctc
     1561 catgcgcgac acacgccaaa cccattaatt tcattagatt tatttaaaac ccgcactttc
     1621 tcgatcggta tcgtaggcaa tattgcaacc cgtctgggga ccggttgtgt accgttcctt
     1681 atgccattga tgttacaggt aggatttggt tatcaggcgt ttattgccgg ctgtatgatg
     1741 gcaccgacag cgttaggttc cattattgca aaatcgatgg ttacccaagt cttacgtcgt
     1801 ctgggctatc gccatacgtt agtggggatc acggtgatta ttgggctaat gatcgctcag
     1861 ttctctttgc aatcaccggt aatggcgata tggatgctga tcttgccgtt gtttatatta
     1921 gggatggcta tgtcgacgca atttaccgcg atgaatacca tcacacttgc cgatctgacc
     1981 gatgacaacg ccagcagcgg taacagtgtt ctggcggtca cgcagcaact gtcgattagt
     2041 ttaggcgttg ctgtaagtgc ggccgtcctt cgcgtttatg aagggatgga aggcacaacg
     2101 actgtcgaac aattccacta tacgtttatc acgatgggca ttattactgt tgcttcagca
     2161 gcaatgttca tgcttctgaa aacaaccgat ggtaataatt tgatcaaaag acagcgtaaa
     2221 tctaagccga accgcgttcc atcagaatcg gagtaagttg taatcgttgt tgctgaaggg
     2281 tcggctgggt tatccgatgg atgagtacat caatcgccag ctcccccagt tcatctttcg
     2341 gttggtggat agtggttaat ggtggcgtca taaagcttgc cagttcgata tcgtcatagc
     2401 caatcaccgc gatatcctgc ggaacctgta actctgcctg atataacgcc tggtaaacgc
     2461 caacagccat agcgtcattt ccggtaaaga cggcctgagg acgcagcgga tgtgataaca
     2521 gttggcgcat ggcgtcaaac ccgccgttaa attcaaaatc accagtgact tcatagccat
     2581 caggaatgtt gagacccgca cgtttcatcg ccgcccgata accttccaac cgcaggcgcg
     2641 ccggagtttt atccagcgga ccggtaatac aggcgatacg ggtatgacct ttatcgatca
     2701 gatattgcgt tgctaagtct ccgcccagca acgagttatc ctgaataaga tcgctgtcgc
     2761 catcgaacgg agcccagtcc atcatcacag taggcactgt cggataacgt tgcatgattt
     2821 cacgcgaagg ttgatgcgtt tcggtgcaca gtaacagcaa gccatcaacg cgtttttgca
     2881 tcagcgtttc cagattgcga ttcatccgct gttcatctcc ttcggtattg caaaggacga
     2941 gactataacc gcgttcgaag cagctgcgtt caacgccacg caccagttct gaatagaaag
     3001 gattggtact ggcagtgatc aacatgccaa tggtatgtgt ttgattgagt ttgaggctac
     3061 gcgccagggc tgatggcgcg taattgagtt ctttaatcgc cgcttcaact ttggcggtaa
     3121 tcgcttcact gacgaagcga tctttattga taacgtgaga aactgttgag gtagaaacgc
     3181 ccgccaggcg ggcaacatct ttcattgtag ccaagcgtca cctctgcctg tctaaaaatg
     3241 cgtcgatctc ttcacgccac ggtacggaag gttgtgcgcc tttacgtgtt acggcaatcg
     3301 cagcggcagc atgggcaaac cgaatcgcct ctggcaatgg tttttcttcc agcaacgccg
     3361 tgattaacgc gccgttaaag gtatctccgg cagcaatggt atcgacagcc tgcacccgga
     3421 atccaggaac gcgctgacct tcaccattca cgctagccca tacaccacga cttcctaaag
     3481 taatcagtac agtacggata cctttttcat gcagtacctg cgccgccttc gctgcatctt
     3541 catcattttc aacacgaata ccggtgagct tttctgcttc cgtttcgttt ggcgtaataa
     3601 tgtccaccag cgccagcagt tcgtcaggaa gttcgcgagc cggagccggg ttaagcgcaa
     3661 cgatagtctt attttgatgg gcgattttcg ccgctgccat cacactttcg agtggtgatt
     3721 ccagctgcat taataatgct gacgcgttgg caatacgctc acgttgcgct tccaccagcg
     3781 ccggggaaag ggcagcatta gcgccggcat gaataccgat gacattctca ccttcgccat
     3841 taacaaaaat cagcgccaca cctgttgatt cgcctttgat cacgctgacc ggagtaatat
     3901 caatgttatc agtggcgagc tgctggcgaa cgctctcacc aatgctgtca tcacccgtac
     3961 aggcaataaa cgcgatattc gcaccgctac gcccagcagc cacagcctga ttcgcgcctt
     4021 tgccgccaaa tgcaacctga tagtggttac cggttacggt ttcgcctgga gtaggaaaag
     4081 attgaagatt aagaatgtgg tcagcattaa tgctgccaag aacaacgagg ctgcctgcgt
     4141 tttgcatatt cgggatgtcc ataaaatgcg ccaccgtgtt agggtggcgc gtgccctgct
     4201 tttctttata ttacacgttc atctgtcgat gacgtattta tgtcaccatc aggtcataca
     4261 acctgattaa aactactgct taacaaccag tttcagatca accggatact tagcctgaac
     4321 tttctcgcct ttcagcactt tatctgcggt ttcgacgcct ttcgcgccaa tctgatcggg
     4381 tagctgagcg atagtcgctg ctagtttgcc atcattcacc gctttttcgc catccggtgt
     4441 accgtcaaat ccgacgacca tcacatccga tttaccggca gtttgcagtg cgcgcagcgc
     4501 gcccagcgcc atttcatcat tctgcgcgaa tacagcctga acatccggat gagcggtcaa
     4561 caggttctgc attacgttca aacctttagt gcgatcaaaa tctgctggct ggctggcaag
     4621 aacattaaac ttgtgagcag caacggcctg ctggaagcct tcgccacgtt cacgggctgc
     4681 ggatgtacca gcaatgcctt gcagctcgat aactttggca ccttcacccg ctttcttcgc
     4741 gatgtaatca ccggcgattt tgccacccag tacgttatca gaagcaatgt ggctcaccac
     4801 ttcacctttc gttgcctggc ggtcaagagt gataaccggg atgttcgcct ggttagccat
     4861 cttcacagca ttacctactg cgtcggagtc ggtcgggtta atcagcagaa ttttggtgcc
     4921 gcgaacggtt aagtcctgca cgttcgccag ctctttcgcc gggttgttct gggagtccag
     4981 caccaccagg ttatagccaa gcttatccgc ctctttctgc gcgccatctt tcagcgatac
     5041 aaagaacggg ttgttaagcg tggagaccac cagcgcgatg gtgtcttttg ccatcgcatt
     5101 cgcactgacg gtggcgctta gcgcaacagc ggaaaccagg gtagccagtt ttttcatgtt
     5161 catattcaag atgtcctgta gtcgttatta ctgctttttg ttgtctacca gcaccgccag
     5221 caaaatcacc accgctttga cgatcatctg gtaataggag gaaacaccta acaaattcaa
     5281 tccattatta aggaagccaa gaattaatgc gccgatcaac gtcccaacaa tgcgaccttt
     5341 tccgcccgcc agactcgtac cgcccagaac caccgcagca atagcatcca gctcatagcc
     5401 agtccccgcc gtgggttgtg cggaggagag acgcgccact tcaatgatcc cggccagcga
     5461 tgccagcaga ccacaaagag aatagacgat gattttgatt ttattgacgt tgataccaga
     5521 aagacgcgtt gccgcttcgt tgccgcccag cgcgtagatg taacgcccca gacgcgtgtg
     5581 atgcagcatg taccaggccg cgaggaagac aatccccatg atccagactg gcgtcggtac
     5641 gcccagcgga cgaccaatac caaaccagcc aaacagatcg gcgttctcag taaagccggt
     5701 attcactggg ctaccgttgg tataaaccat ggtcacgccg cgcagtaaaa gcatcataac
     5761 cagcgtagcg ataaacgcct gaacgcgacc tttcgctaca atcaccccgg ttaccgcacc
     5821 aattgcggca cctaacgcga gagcggcagc gacagccacc agcgcattga cttcaatgcc
     5881 gacgatagat gcagcaactg cgccggttag cgccaacaga gaacctacag acaagtcgat
     5941 gcccgacgtc aggatcacca gcgtcatccc gaccgccata atggcgttca ccgaggtttg
     6001 ctggagaata ttgaataagt tattgatggt gaaaaagttc gggcttaacg tcgagacaat
     6061 cgcgatcagc accagcagag cgataagcga tttctgctcc atcagccacg ctttcgtgaa
     6121 ataacggcga ccagagacag tctgggttgt cattttttta ctcctgattc acgcgattaa
     6181 gcttgcccac agcggcagcc attaacactt cctgggtggc ctgctcacga gtaaattccc
     6241 cgctgagatg cccttcatgc atgacgatga tgcgatcgct catgcctaat acttctggca
     6301 tctccgatga caccagaatg atgctcaagc catcggcttt gaactggtta atcagttgat
     6361 agatctcttt tttcgcgccg acatctacgc cacgggtagg ttcatcaagg atcaacactt
     6421 tggggcgtgt catcagaccg cgggcaatcg ccactttttg ctgattgcca ccggaaagca
     6481 gaccaattgc ctgttccatc gacggtgttt tcacattaaa cagacgaatg aaatcactca
     6541 cagcctgctg ttcatcggca tgcttcaaac tgccgccagc gcggctgaag tagcgcagcg
     6601 ctgtcagcga catgttctct tttactgaca tgcccaacac taaaccgtca cgtttacggt
     6661 cttcggagat atacacaatg ccgtttgcca ggccatcctg cggtgaacgg gtaacgactt
     6721 catgcccatc cagggtgacg taaccgctgg tgcgcggtag tgcgccgtag agcactttca
     6781 tcagttcggt acgacctgcg cccatcaaac cagagacgcc aagaatttcg cctttgcgta
     6841 aagtaaaaga gacatcgtta acgccaggtc cgcagagatt atcgactttc aggcggatat
     6901 ctcccggcgc tttgtccagg tgtggatatt gatcttccag cttgcggccc accatcatct
     6961 caatcagcga atcttcggtc agtgatgcca cttcgcgctc agcaataaat tgcccgtcac
     7021 gaaaaacggt aacgtcatcg caaatctcga agatttcttt catgcggtgg gagatataga
     7081 caataccgcg gccttgcgat tttagttcgc ggatgacgcg gaacagggat tcggtttcgg
     7141 tatcggtcag cgcatcggtc ggttcatcca taatgatgac tttcgactca aagctcagca
     7201 ctttggcgat ttcaaccatt tgctggtcac cgatggaaag atcgcccacc agcttgtcgc
     7261 ttttaaagcg caggttaagt ttagccagca atttatccgc ttcggcatac atggttttcc
     7321 agtcaatttt gccaaagcga ttaacaaact cacgaccgag gaaaatgttt tcggcaatgg
     7381 tcaactgcgg gatcaggttc agttcctgat ggataatccc aatcccggct tcctgggaag
     7441 atttcggccc ggtaaatgtc gtttctttcc ccagccataa aagcgtgccg gcatcgcgag
     7501 catagatacc agtaagcact ttcatcatgg tggatttacc cgcgccgttt tcgcccacca
     7561 gcgccatcac gcggcccgga tagacattta acgctgcgcc cgagagggct tttacgcccg
     7621 ggaaggcttt atcgatgcct ttaagctgaa gtaatgcttc catgacggcc tcagaacgtc
     7681 acgccagcac agagaatgat attcgcatac ggagaacatt ctccgctgcg aattaccgcc
     7741 tgactttctg cggtttgttg tttgaattgt tcatgcgtgg tgtaacgaat ttcaatggta
     7801 tttccctggt gtttttgcag ctgctcaagg tgagtgagca acgtttcgtg gagttgcgga
     7861 ttatgttgtt tgatctcttc cgcgataatg gcagcctcga cctgcatttc atttgtgacg
     7921 acgcccagca cctgcataaa agaaggtaca ccctgggtta atgccatatc gatacgcgtt
     7981 gtacttttgg ggataggtaa accagcatca cacaccacca gcgtatcggt atgtcccaga
     8041 cgggagatca ccgatgaaat atcagaatta agaacggtgc cttttttcat ttttttctcc
     8101 atcagcgaaa cgtttcgctg acccgcagtt taatcctgac tgagttggga aaaccatcgt
     8161 gacgttacgg aaatgtgatc aacctcgaaa cgttttacat ggtgattaac catgaaaaca
     8221 aaaacgcccc cttatggaga aaggaggcgt ctggcgttag atttcgacct gagtacccag
     8281 ttcgataacc ctgtttggcg gaatttcaaa ttgatctggt gcacgcagcg cattacgttg
     8341 cagcagcaag tacagcttgc cgcgcagacg caaataccac gggcgtttgc cgaggatcaa
     8401 cgactcatgc gacataaaga aggaggtttc catcatccgg caacttaatc cttccagacc
     8461 gcagcggtgg aaaacttctt ccacgtttgg cgtttctcgc caaccataac ttgccaccac
     8521 gcgccagaaa gtgggcgaca gttgttcaat ctgtacccga cggacattat ggacatatgg
     8581 cgcgtcttcg gtacgcagag ttaacagaat cacccgctca tgcaatacct tgttatgttt
     8641 aaggttatgc atcagcgcaa agggaatgac gttgattgca cgcgacatat acaccgcggt
     8701 cccgggcacg cgaacaggcg gtgatttctc cagcgaagca atcatcgctt ccagagagtt
     8761 accatgttca tgcatccgcc gcagcaagcg gaaacgctcg ctcttccagg tggtcatcac
     8821 gataaacatc acagtaccga ggctcaatgg caaccagccg ccggagagca gtttatcgag
     8881 gttagcggtg aacaatggaa tatcgacaca aaggaaagca atcaggatca gcgcaacaaa
     8941 atacttattc cagtgccagt tctgacgtgc cacggtagtc gagagaatag acgtcagcac
     9001 catggttccg gtcaccgcaa tcccgtacgc cgccgccagg ttgctggagt gctcaaagct
     9061 gacaatcaca atcacgaccg cgacatagag catccagttc acaaacggaa tatagatttg
     9121 ccctgactcc atttcggagg tgtgaataat gcgcatcggc gacaaatatc ccagacgtac
     9181 cgcctgacgc gtcaatgaga agacgccaga gataaccgcc tgcgaggcaa ttaccgtcgc
     9241 cagtgcggcg atgatcagca gcgggatcag cgcccagtcc ggtgccaaca ggaagaacgg
     9301 gttcttaatc gcttccgggt tctttaacaa cagcgctccc tgaccgaagt aattaagggt
     9361 caaggaaggt aatacgacgg tgaaccacgc caggcgaata gggaacttac caaagtgccc
     9421 catatcagca tacagcgcct cgacccccgt aatcgacaac accactgccc ctaatgcaat
     9481 aaaagaaacc gttttgtatt caaggaagaa atgcaccgcc cacattggat tcagcgcatg
     9541 cagcacttcc gggttagcaa taatgctacg taaccccagt cctgccagaa tcaaaaacca
     9601 ggtcagcatg atcggcgcaa acagcttacc gaccatagcc gtgccatgtt tttgaatcat
     9661 aaataataaa gtgagaacga taattgagag gggaactatc caggtatcca gctgcggggc
     9721 gacgatttcc agaccttcaa tggctgacat caccgaaata gcgggtgtta tgacgacttc
     9781 accatagaaa aagctgccgc cgattagccc cataatcacc agcattgatg tggttcgcgc
     9841 cgacgtatta cgcccggcaa gcgacatcaa cgtcaggatc cccccctcac cggcgttatc
     9901 tgcccgcatc acgaaggtga gatatttaat ggaaaccaca aagattagca gccagaagat
     9961 cagcgataaa aagccaaaca cggtatcgcg ttcaacgcca aaaccaaact ggccggacaa
    10021 acattcacga agtgtatata acgggctggt accaatatcg ccgtagacaa ctccaatcgc
    10081 cgcgagggta atcgcgggca atgattgctt attatcagtg ctcatagact agtctttcgt
    10141 tgaaatatga aatgtgtgct tagtcccttg gcccacaaaa aagcgcacag tatgcacgat
    10201 taacggcaaa atcgtactcc taaatgcggc cacattaacc tggctcaaag aaaaataccc
    10261 cccctttgag actattttag ccaccagcaa gaaacgtcta tactcgcaat ttacgcagaa
    10321 cttttgacga aaggacgcca cttcattatg gctcaccctc atttattagc ggaaagaatt
    10381 tcccgcctga gcagttcgtt ggaaaagggg ctttatgaac gtagccacgc catccgcttg
    10441 tgtttattag cggcattaag tggtgaaagt gtgttcctcc ttggcccgcc aggtattgcc
    10501 aaaagtttga tcgcccggcg cttaaaattc gcctttcaga atgcccgcgc gtttgaatat
    10561 ctgatgaccc gcttctccac gccggaagaa gtttttggtc ccctttctat tcaggcgcta
    10621 aaagatgaag ggcgctatga acgtttaacc agcggttacc tgccggaagc agaaattgtc
    10681 tttctggatg agatctggaa agcgggcccg gcaattctta ataccttgct caccgccatt
    10741 aacgagcgcc agttccgcaa cggtgcacac gtagaaaaaa tcccgatgcg tctgctggtg
    10801 gcggcctcca acgagctgcc ggaagcagac agcagtctgg aagcgttata tgaccgcatg
    10861 ctgattcgtc tgtggttaga taaagtgcag gataaagcga atttccgctc catgctgacc
    10921 agtcaacagg atgaaaacga caatccggtt cctgatgcct tgcaggtcac agatgaagaa
    10981 tatgaacgct ggcagaaaga gattggtgaa attacgctgc ccgatcatgt atttgagctg
    11041 atttttatgc tgcgccagca actggataaa ttaccggatg cgccttatgt ctcggatcgt
    11101 cgctggaaaa aagcgatccg attattgcag gccagcgcct tttttagcgg tcgcagtgct
    11161 gttgccccgg tagatctcat tttgctgaaa gattgcctgt ggtatgacgc gcaaagcctg
    11221 aatttgatac aacaacaaat tgatgtattg atgaccggtc acgcctggca acagcaaggg
    11281 atgttgactc gccttggcgc gattgtgcaa cgtcacctgc aactccagca gcaacaaagc
    11341 gataaaacag ccttaacggt aattcgtctg ggcggcattt tcagccgtcg tcagcagtat
    11401 caacttcctg ttaacgttac tgcttccact ctgactctac tgctgcaaaa accgttaaaa
    11461 ctgcatgata tggaagtggt tcatatctcc tttgagcgta gcgcgctgga acagtggctg
    11521 agcaaaggtg gtgaaattcg cggtaaactg aacggtatcg gctttgccca gaaactgaat
    11581 ctggaagttg atagcgccca acatcttgtc gttcgcgatg taagtttaca aggcagtacg
    11641 ctggcacttc ccggttcatc ggctgaaggt ctgcctggtg aaataaaaca acaactggaa
    11701 gagcttgaaa gcgactggcg caagcaacac gctttattca gcgaacagca aaaatgtctg
    11761 tttatccctg gcgactggtt aggtcgcatt gaagccagcc tacaggatgt cggtgcacag
    11821 attcgccagg cacaacaatg ctaacgctgg atacgcttaa tgtgatgctg gccgtcagcg
    11881 aagagggatt gatcgaagag atgatcatcg cactgctggc ctcaccgcag ctggcggtct
    11941 tctttgaaaa attcccacgc ctgaaggcgg caatcactga tgatgttccc cgctggcgtg
    12001 aagcgctgcg cagtcggctg aaagatgccc gagtcccgcc ggaactcacc gaagaggtga
    12061 tgtgctatca gcaaagccag ctcctctcca cgccacagtt tattgtgcag ctaccacaga
    12121 tcctggactt actgcatcgt ctgaattctc catgggcaga acaagcccga cagttggttg
    12181 atgctaacag cacgatcact tcagcgttac acacgctttt tctccagcgt tggcgtttaa
    12241 gtctgatcgt gcaagcaacg acgttaaatc aacagctatt agaagaagaa cgcgaacaac
    12301 tgttgagtga agttcaggaa cgcatgacgc tgagcggaca acttgaaccg attctcgcag
    12361 ataacaatac tgcagctggt cgtctgtggg atatgagcgc cggtcagctt aaacgtggcg
    12421 actatcagtt gattgtgaaa tacggtgaat ttcttaacga acagccggaa ctgaaacgcc
    12481 tggcagagca gctggggcgt tctcgggaag ccaaatcaat accgcgcaac gatgcgcaga
    12541 tggaaacctt ccgcaccatg gtgcgcgaac cggcgacggt tcctgagcag gttgatggtc
    12601 tgcaacaaag cgatgatatt ttacgtcttc tgccgccaga actggcgaca ctagggataa
    12661 cggaactgga gtatgagttt taccgtcggc tggtggaaaa acagttgctc acctatcgcc
    12721 tgcacggtga gtcgtggcgt gaaaaagtga tcgaacgtcc ggtggtacat aaagattacg
    12781 atgaacagcc gcgcgggccg tttattgtct gtgtggatac ttccggctca atgggcggct
    12841 ttaatgaaca gtgtgcgaaa gcgttctgcc tggccttgat gcgcattgct ctcgctgaaa
    12901 accggcgctg ctatattatg ctattttcca ccgagatcgt ccgttatgag ctttcaggcc
    12961 cacaaggcat cgaacaagca atccgttttt taagccagca gtttcgtggc ggcaccgatc
    13021 ttgccagttg ttttcgcgcc attatggaac gcttgcaaag cagggaatgg tttgatgccg
    13081 atgcggtggt gatttctgat tttatcgctc agcggttgcc tgacgacgtg acgagtaaag
    13141 tgaaagagct gcagcgggta catcagcatc gctttcatgc cgtggcgatg tcggcacacg
    13201 gcaaacccgg catcatgcgc attttcgatc atatctggcg ctttgatacc gggatgcgaa
    13261 gccgcctgct cagacgctgg cggcgataaa ttattacagc agagaaggga cgctctcgcg
    13321 aacagcagct ggccatactc cacactgaac ctggccgata tgcggcagtt gcagcagcag
    13381 catggtcaaa cgagactggc cgataccgcc gccgatggtc tgcggcattt caccgcgcag
    13441 cagcgcctga tgccattcca gctgcagacg atcttcatca ccggtcagcg ccaactgatg
    13501 cttcaacgtg tcggcatcca cgcggatccc catggaagaa agctcaaacg catcttccag
    13561 tacagggttc cataccagaa tatcgccgtt cagacccgca tggcccagct ctgacggggt
    13621 gctccagtca tcataatccg gtgcgcgcac gtcgtgacga tgaccatcac tcagcttgcc
    13681 gccaatcccg acgaggaata ccgcgccaag atctttcgca atcgcccgct cacgcccttt
    13741 ggcatcgaga tccggataac gagacagtaa ctcctggctg tgtacgaagt ggatctgatc
    13801 cggcaggaac ggtgccaaac caaactcttc gctaactgca gcttcggttg ctttaattcc
    13861 cgcccagatc gcctctaccg tgcttttcag agtcgagaat tgacgctcac cgtcgcccat
    13921 tacgcgttcc cagtcccact ggtcaacata gaccgagtgc aacggagaaa gacgctcttc
    13981 atcggggcga agggctttca tgtgcgtgta cagcccttcg cccgcgctga agtcgtgttg
    14041 ccctaaggtc tgacgtttcc acttcgccag tgaatgaacc acttcgaact gggcatcagg
    14101 cagagctttc acttttacct gcaccgcttt ttcacagccc gacaagttat cctgcgtgcc
    14161 atcccccaca cggctaagaa tcggcgcctg gacttcgatc agccccagac gttcttccag
    14221 ttggcgagaa aagtgagatt tcacgaagct aatttggcgt tgtttggcaa tgtaagcggt
    14281 tttcattttt tatactcctg cgtcctgttg cttatgatta agcaacaaaa accgacacac
    14341 atgcaataat cattcaataa aaagcctaac acacttttga ttcattaaaa aagaaggcta
    14401 aaatagaatg aatcatcaat ctgcataaga aaatcctatg gaaaattatc tgatcgacaa
    14461 tctggaccgt ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt acgccgaact
    14521 ggcgaaacaa tttggcgtca gtccggggac gattcacgtt cgagtagaga aaatgaagca
    14581 ggcggggatc attaccgggg cgcgtattga tgtcagcccg aagcagctcg gttatgacgt
    14641 aggctgcttt atcggcatta tattaaagag cgccaaagac tacccttccg cgctggcaaa
    14701 gctggaaagc cttgatgaag tcactgaagc ctattacaca accggccact acagcatctt
    14761 tataaaagtg atgtgccgtt cgatcgacgc tctccagcat gtacttatca acaagatcca
    14821 aacaattgat gaaattcagt ccaccgagac attgatcgtc ctgcaaaacc cgatcatgcg
    14881 taccatcaag ccataatcgg cttttttaat cccatacttt tccacaggta gatcccaacg
    14941 cgttcacagc gtacaatacg ccactcttaa taaaggtggc ggtttatggc agatatcact
    15001 cttatcagcg gcagcaccct cggcggtgcc gaatatgtag cagaacacct ggctgaaaag
    15061 ctggaagagg cgggttttac caccgaaacg ctgcacggtc cgctgttgga agatttatct
    15121 gcctcgggga tctggctgat tatcagctcc acccacggtg ccggagatat tccggacaac
    15181 ctttctcctt tctatgaagc attgcaggaa cagaagcccg atctttctgc ggtccgcttt
    15241 ggcgcaatcg gtattggtag tcgtgaatat gacacctttt gtggggctat cgataaactc
    15301 gaggcagaac tcaaaaattc cggtgcaaaa cagacaggcg aaacactgaa gatcaacatt
    15361 cttgatcacg acattccgga agatccggca gaagaatggc tgggatcgtg gattaattta
    15421 ctcaaataag tatacagatc gtgcgatcta ctgtggataa ctctgtcagg aagcttggat
    15481 caaccggtag ttatccaagg aacaacggtt gttcagtttt tgagttgtgt ataacccctc
    15541 attctgatcc cagcttatac ggtccaggat caccgatcat tcacagttaa tgatcctttc
    15601 ccggttgttg atcttaaaag ccggatcctt gttatccaca gggagtgcga tcctaataag
    15661 agatcacaat agaacagatc tctaaataaa tagatcttct ttttaatacc caggatccca
    15721 ggtctttctc aagccgacaa agttgagtag aatccacggc ccgggcttca atccattttc
    15781 ataccgcgtt atgcgaggca atcaccatgt tttatccgga tccttttgac gtcatcatca
    15841 ttggcggggg tcatgcaggc accgaggccg cgatggccgc ggcgcgtatg ggtcaacaga
    15901 ctctgctttt gacacacaat atcgacactc tggggcagat gagctgcaac ccggcgatcg
    15961 gcggtattgg gaagggacat ctggtaaaag aagtggatgc actcggcggt ctgatggcga
    16021 aagcgatcga tcaagcgggt atccagttta ggatactaaa cgcaagcaaa ggaccggcgg
    16081 ttcgcgctac ccgagctcag gcggatcgtg tgctctaccg tcaggcggta cgtacggcgc
    16141 tggagaacca accgaacctg atgatcttcc agcaggcggt tgaagatctt attgtcgaaa
    16201 acgatcgcgt ggtcggtgct gttacccaaa tgggactgaa gttccgtgcc aaagccgtcg
    16261 tgctcaccgt tgggacgttc ctcgacggta aaattcatat cggtctggat aattacagcg
    16321 gtggccgtgc tggtgatccg ccgtccattc cgctttctcg ccgtttgcgt gaactgccgc
    16381 tgcgcgttgg tcgtctgaaa accgggacac caccgcgtat tgatgctcga actatcgact
    16441 ttagtgtgct ggcgcaacag catggcgata acccaatgcc ggtattctcg tttatgggca
    16501 atgcgtccca gcatccccag caggtgccgt gttatatcac tcataccaac gagaaaaccc
    16561 atgatgtgat ccgcagtaac ctcgatcgta gcccaatgta tgcaggggtg atcgaaggtg
    16621 tcggcccacg ctactgcccg tcgatcgaag acaaagttat gcgcttcgcc gacagaaatc
    16681 agcatcagat cttccttgaa ccggaaggac tgacctctaa tgaaatttat ccgaacggta
    16741 tctccaccag cctgccgttc gatgtgcaga tgcaaatcgt ccgctctatg caggggatgg
    16801 aaaacgcgaa gatcgtgcgt ccgggttatg ccattgagta tgacttcttc gatcctcgcg
    16861 acctgaaacc gacgctggag agcaagttta tccaggggct gttctttgct ggtcagatta
    16921 acggcactac cggttacgaa gaagccgctg cgcaaggttt gctggccggt cttaacgctg
    16981 cccgtctgtc tgctgacaaa gaaggttggg ctccggcacg ttctcaggcg tatctcggcg
    17041 tactagttga tgacctgtgc actttaggaa ccaaagaacc gtatcgtatg tttacttcgc
    17101 gcgcagaata tcgtctgatg ctacgcgaag ataatgcgga tctgcgtttg actgaaatcg
    17161 gtcgtgaact gggcctggtg gatgacgaac gttgggcgcg ctttaacgag aaacttgaga
    17221 atatcgagcg tgagcgtcag cgtctgaaat cgacctgggt aaccccgtcg gcggaagctg
    17281 cagccgaagt gaatgctcac ctgactgcgc cgctttcccg tgaagccagt ggtgaagatc
    17341 tgctgcgtcg tccggaaatg acttatgaaa aattaaccac gctgacgccg tttgcccctg
    17401 cgttgacaga cgaacaggcg gcggaacagg ttgagattca ggttaaatac gaaggttata
    17461 tcgcgcgcca gcaagatgag atcgaaaagc agctgcgtaa cgagaacacc ctgctacccg
    17521 cgacactgga ttaccgccag gtatccggtc tttctaacga agtgatcgcc aaacttaacg
    17581 atcacaaacc agcctctatc ggccaggctt cgcgtatttc tggcgtcacg cctgcggcca
    17641 tctccattct gctggtgtgg ctgaaaaaac agggtatgct gcgtcgtagc gcataacgca
    17701 ttaaaaatgc ctggtaagca cccgcttacc aggcaacgca tcaagaacag gtaatcaccg
    17761 tgctcaacaa actctcctta ctgctgaaag acgcaggtat ttcgcttacc gatcaccaga
    17821 aaaaccagct tattgcctac gtgaatatgc tgcataaatg gaacaaagcg tacaacctga
    17881 cttcggtccg cgatcctaat gagatgctgg tacgccatat tctcgatagc attgtggtgg
    17941 caccgtatct gcaaggtgaa cggtttatcg atgtcggcac cggaccagga ctgccaggca
    18001 ttccactctc tatcgtgcgt cctgaagccc atttcactct gttggatagc cttggtaaac
    18061 gcgtgcgttt ccttcgtcag gtgcaacatg agcttaaact ggagaatatt gaaccagtac
    18121 agagcagggt agaagagttt ccttcagagc cgccatttga tggcgtaatt agccgcgctt
    18181 ttgcctctct gaacgatatg gtgagctggt gccaccatct tcctggtgag caaggccgtt
    18241 tctacgcgct gaaagggcaa atgccggaag atgaaatcgc tttgttgccc gaagaatatc
    18301 aggtcgaatc agtggttaaa cttcaggttc cagccttgga tggcgaacgt catctggtgg
    18361 tgattaaagc aaataaaatt taatttttat caaaaaaatc ataaaaaatt gaccggttag
    18421 actgttaaca acaaccaggt tttctactga tataactggt tacatttaac gccacgttca
    18481 ctcttttgca tcaacaagat aacgtggctt tttttggtaa gcagaaaata agtcattagt
    18541 gaaaatatca gtctgctaaa aatcggcgct aagaaccatc attggctgtt aaaacattat
    18601 taaaaatgtc aatgggt
//
LOCUS       DAZFDP010000091        18602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFDP010000091 DAZFDP010000000
VERSION     DAZFDP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(457..1032)
                     /locus_tag="ACHJ1G_003213"
     CDS             complement(457..1032)
                     /locus_tag="ACHJ1G_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4765015.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(1101..1679)
                     /gene="terD"
                     /locus_tag="ACHJ1G_003214"
     CDS             complement(1101..1679)
                     /gene="terD"
                     /locus_tag="ACHJ1G_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4765016.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1728..2768)
                     /gene="terC"
                     /locus_tag="ACHJ1G_003215"
     CDS             complement(1728..2768)
                     /gene="terC"
                     /locus_tag="ACHJ1G_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4765017.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2791..3246)
                     /locus_tag="ACHJ1G_003216"
     CDS             complement(2791..3246)
                     /locus_tag="ACHJ1G_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4765018.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3269..4426)
                     /locus_tag="ACHJ1G_003217"
     CDS             complement(3269..4426)
                     /locus_tag="ACHJ1G_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4765019.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4426..5007)
                     /locus_tag="ACHJ1G_003218"
     CDS             complement(4426..5007)
                     /locus_tag="ACHJ1G_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4765020.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            5330..6388
                     /locus_tag="ACHJ1G_003219"
     CDS             5330..6388
                     /locus_tag="ACHJ1G_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4765021.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            6398..7540
                     /locus_tag="ACHJ1G_003220"
     CDS             6398..7540
                     /locus_tag="ACHJ1G_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4765022.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7533..8306
                     /locus_tag="ACHJ1G_003221"
     CDS             7533..8306
                     /locus_tag="ACHJ1G_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765023.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            8308..9387
                     /locus_tag="ACHJ1G_003222"
     CDS             8308..9387
                     /locus_tag="ACHJ1G_003222"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4765024.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            9387..10343
                     /locus_tag="ACHJ1G_003223"
     CDS             9387..10343
                     /locus_tag="ACHJ1G_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4765025.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            10354..11562
                     /locus_tag="ACHJ1G_003224"
     CDS             10354..11562
                     /locus_tag="ACHJ1G_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO4765026.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISRAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11580..12047
                     /gene="terW"
                     /locus_tag="ACHJ1G_003225"
     CDS             11580..12047
                     /gene="terW"
                     /locus_tag="ACHJ1G_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4765027.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            12308..12637
                     /locus_tag="ACHJ1G_003226"
     CDS             12308..12637
                     /locus_tag="ACHJ1G_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4765028.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            12624..13004
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_003227"
     CDS             12624..13004
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4765029.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            13047..13877
                     /locus_tag="ACHJ1G_003228"
                     /pseudo
     CDS             13047..13877
                     /locus_tag="ACHJ1G_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(13908..15521)
                     /locus_tag="ACHJ1G_003229"
     CDS             complement(13908..15521)
                     /locus_tag="ACHJ1G_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4765030.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(15552..15902)
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_003230"
     CDS             complement(15552..15902)
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4765031.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(15899..16324)
                     /locus_tag="ACHJ1G_003231"
     CDS             complement(15899..16324)
                     /locus_tag="ACHJ1G_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4765032.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            16378..17129
                     /locus_tag="ACHJ1G_003232"
                     /pseudo
     CDS             16378..17129
                     /locus_tag="ACHJ1G_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            18446..>18602
                     /locus_tag="ACHJ1G_003233"
     CDS             18446..>18602
                     /locus_tag="ACHJ1G_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765033.1"
                     /translation="MYLHTYQHNDRLPPQKHDKGIIMEFSVIFFWVITGLITILFLKS
                     IITDLIKN"
BASE COUNT         4559 a         4884 c         4719 g         4440 t
ORIGIN      
        1 tgaaggggta tgttttgtcc ggattgtaaa ttcatattaa ctctcctgat ttttgattat
       61 tattaatgcg cagcgtttat atatgttccc taggcttagt tctggacgct ggatattcgg
      121 tgaggcgtaa atatggtatg acaccatttt tcataacgct gaagtttcta tacctgttga
      181 atttgaattt tcattgaccg ggtatcttat tttccagggc ccgttccttc ataagtcgca
      241 aaagtaacat atatccgaag ggcatgctgt tgatatcaga cacggaatac tggctttaac
      301 cagcaaccac agataaaccc ggggcctgca taaaaggttc atgccagaat taacatagct
      361 ctgctttctg cccccccctt tcccatattc acccgttgat agcggatcga tacccaaaaa
      421 aaaccccggc attgccgggg tcagactcag cgtcttttag acattgacgc cgtgctggga
      481 agcaagcgct gacagaccac cggcaaaacc ctggccaaca gctttaaact tccactcagt
      541 accatggcga tacagttcac cgaagaccat tgcggtttcg gttgaggcgt cttcagacag
      601 atcgaaacgg gcaatttccg tcccgttgtc gttgttgtaa acgcgcatga agctgttgct
      661 caccatgccg aagttttgtt tacgcgcttc tgcatcatag atggtaacgg caaataccag
      721 ttttttgatg tctgctgaga ctttggtcag atcgattttg acctgctcat cgtcgccgtc
      781 gccttcaccg gtacggttgt cgccctggtg ctctactgcg ccatcagggc tggttttatt
      841 attgaagaaa atgaaatggg catctgacag tactttaccg tcttcaccta ctgcgaatac
      901 ggaagcgtcc agatcaaaac cctgaccatc ggttacacgg gcatcccagc ccaggccaac
      961 catagcgaca ttcatggttg gtgcttcttt ggtcagagat acgttgccgc cttttacgag
     1021 agaaactgcc atttttagct cctgcaaaca gttgaatgag gctgaattaa cagccccaga
     1081 tatgaaaagt tacttttcga tcaggacgcg ttaatgccgt actgagcaca tacagatgcc
     1141 agaccaccag cataaccctg gcctactgcg cggaatttcc actcaccatt gtggcgatac
     1201 agctcgccga acagcatggc agtctcagtg gacgcatctt cggtcagatc gtagcgagcg
     1261 acttcagtct ggttatcgtc attaaccaga cgaataaacg caccggatac ctgaccaaag
     1321 ctctggcgac gagcctgagc atcgtggatg gtcacaacga agatgatctt gtcaacttca
     1381 gacgggacgg cgtccagttt aattttcagc gattcatcat caccatcgcc ctcaccggtg
     1441 cggttatcgc cggtgtgcgt tacggaaccg tcggatgacg tcaggttgtt atagaagatg
     1501 aaatctgaat cgccgcgcac tttgccgttt gaggccagca ggaatgctga agcatccagg
     1561 tcaaagtcct gaccgtctgt tgaacgcgca tcccagccaa ggcccaccag gacatttttc
     1621 attgacggag ctgctttact cagggagacg ttcccgcctt tggaaagaga aacactcata
     1681 aaataacctc ttcgattagt aattgttcag gttaacactt aaggggatta actccccttt
     1741 tcctcagatt caggtttgcc cgggaacatg acgcttgcga gaatgcccag cgccagtaca
     1801 cccagtacaa catacaggct ggttgttgcc gcgatgctgt aaccatgatg ccagatgtga
     1861 tcggtcgcat tcaggccgag ttttgccgcg atgaagaaca gcagcacgat aacggccttc
     1921 tccagatgaa ccaggtactg tttcagtgcc tcaaggacaa aatacagagt acgcaggccc
     1981 aggatagcaa acatcatggc actatagacg ataagcggtt cacgactgac ggcaatgatt
     2041 gccggtaccg agtcaaacgc gaacattacg tccgagagtt caaccacagc cacacacagg
     2101 aacagcgggg tcgcatacag cgccgctttt ttaccacggc caatggtgac atcgctgttt
     2161 tctggtttcg ccagttcagc atccacttcc ttctggttaa gtaggaaggc atgccctctg
     2221 agcttcggcc agatagggaa gaagcgttta accatacggt aagccagatg ctgggagtaa
     2281 tcctcaattt catcatcgtc atcaccgctt ttaagcatca tgaccgccgt ccaggcaacg
     2341 ataatagcga agacaacttc aacgtacggc cccagactca gcaggctcgt accgatggcg
     2401 acaaagatgc ccctgaagac gatggcacca atgatccccc agtagagaac acggtggcga
     2461 taacgatccg gaacggcgaa ccaggagaag attgccatca tgacgaacag gttatcgacc
     2521 gacagcactt tctccagcgc ataacccgtg acaaacagac tggcaacctc agcaccgtgg
     2581 tggatataga ggaaaccggc aaatgccatc gcaaccacaa cccagaatac ggaccagagc
     2641 gcagcactct tcagcgagat tggcttgtca tcacggtgca taaacaggtc aataaagatg
     2701 gcaccgactg aaagcgcaat aaaaacaatg accgtttcag tcgggaagcc gatgtgtgtg
     2761 gaaaccatat tttaccctta gggatgtgga tcagaggcca aattcagccg ggtcgaagcc
     2821 caacgcgata gcgatctcgc gggaggccag tttctcgtcc tgatcgaagt taccgtcact
     2881 tttcgcaacg gcaataccaa cacgtaaggc cagctgagcg gcctcaggct gatctttcag
     2941 cgccaggatg tatttcatgg tttcgccctt gccaatttca acatcgaaat cgaagcttga
     3001 aaccagttta ttgaagaact caatcacctc attggtatcg aagaccttca gctcttctga
     3061 agagcgcaga aagcccatca ttttctgctt ttcttccgaa cttacgccgt cactcgatac
     3121 ggcaatacgg gcacatacag caacggtgcc ctgcatgaat tttttgtttt tgaaacggcc
     3181 aacctggcgg gtcagttcgt cacggcctga gttaatggca cctttaactt tgtcgaaaaa
     3241 gctcatacct gtttcctttg ttgctgtgtt atttagaacc ggcgctccag cgaaatcccc
     3301 agccaaatgc ccggtccatt tcgtcctggc ctttgaagta ctggttaatt cgctcgactt
     3361 taattgcgcc gttttcgttt acgagtctgg caatggcgca caatgtgcga cggttctcac
     3421 cttcggtcag gcgggtctca atgggcggtt gatccggcac atgaatggtg actacaccat
     3481 cagtcttgtc ccagctggga acaccttcat aaataaaggc gtaaatcagc acttcgcgga
     3541 tatgcttcca ttcacgtcca ttgatatgca gccactcgcc gtctgataca tcacccgtcc
     3601 ggtcatcgcc cttgagctga acatacggtt catcgcgata atcaccgaat gcattaccga
     3661 gcgcctgaat gaccgattta tacccgtcct gaagctcaac aaaggccccc agatccaggt
     3721 cgattccttt ggagccgaac atacctgcaa agcctgattt gctactgccc cggtgccagt
     3781 tcaggttgat ccggatttca ccgaagttat cccgtttggt caggctgatt gcaggcttct
     3841 cttttgtcag cgataccttg ctcaggctga tttttgtctc aactggcgga ggggttacaa
     3901 ccggaggagt tggggctgcg ggagccggtt cgtcggcaat atttacgccg aaatgctctg
     3961 ccagcggttt aagtccaccg ttgaacccct gcgcaacgaa gcggaatttc cagtcattat
     4021 tacgacggta aaattccccc agaattaagg ctgcttcctg acggccgctc agttcgacac
     4081 tgccgctaac caggccggta gccccttgct ccacgtcgat ggaaagatta cgaagaccgg
     4141 acaccgtctg cccaccgtca caggtcacgg tgaacgcaat tttctggaca tcaggtttca
     4201 gccggttgag cgcgacagta aaggttgagt actggccttc actgaccagg ctgacggtac
     4261 catcatcgtt acgtggctga ccataaaaca ccatgtccgc atcaccctgc accttcccgt
     4321 cggcatacag gcgaaacgcc gaagcatcca cctggccgcc cgaggtgatc cggacccgca
     4381 actcctgggc tggaacgggg gcattgcccc ccggcgtcag gttcattagc gaataaccgc
     4441 tgaaacgata tcggagtgca tatcgtcaat tgtgcggccg cgacaggcat ggccaagggc
     4501 ggtaaagtcc cagttgccgt tattacggcg cagcgaagaa ataacgatcc cggtatgtga
     4561 gccctgctca gtcagcttgt agcgagccag ctctttgccg gtttgatcga caacgcggca
     4621 gaaagcgttt tcgacgtcat tgaacgactg accacggaag ctgtttaccg taaacgccag
     4681 gtattcaacg tttgcaggca ggcgggagag gttgacctta atcacttcat cgtcaccgtc
     4741 accctcaccg gtcaggttat cgccactgtg cacaacagcg ccgcaggtgg atttcagttt
     4801 gcggaaccag atggtgtcaa ttgttttacc tgtgctgtcc attaatacac agccagcatc
     4861 gagatcgatt gagtcgttgc ctccaaacaa tcctccaaga agaccttttt tcttaaccgg
     4921 atcccaaccg agaccgaaat gaagctggct taatgcagat gattctttgc tgagtgatac
     4981 cgtctggttc ttaactaatg aaaccatgtt ttattcctta aaatggtaat gaccgagaca
     5041 atcaaaggta tatttagtaa ctgacgaaga aaatcatatc atgatgaagt tctctgacaa
     5101 gggaaatttt tgtggttttc tgtaaaaaca caaaaagaaa tccttttcag ggtgttagtt
     5161 tttctatttt taatttaaat atcaatgtgt tataaggaat ccaaaaaaca ctgacgcact
     5221 ccataaccac aggagttatt aatcatatta tgattgacat gtagcttaga cactcataga
     5281 ctcacttctc aatcataaat ttgcatggcg ttccggataa ttaaaaaaaa tgaacaagaa
     5341 aatttggttt atggaaggtt tatcctccca gcgagatatt attcaggggg taaaatcatt
     5401 tgcacaaaaa aataattttg ccattaccgt ttttgcctcc caccgtaacg aaagaaatga
     5461 aatcctttcc gttgccgatt attctttgac tgaacctgaa gatcctcaaa aacgtcttca
     5521 gtttatccag gaaaccattc agacctacgg catccaccat attcatactg gccgtaacag
     5581 ccagtggttt gaagaacacc gttcagccat tgaaccgacc ggtgccaccc ttactaccgg
     5641 tgcaacgggc gtcgactggt taactctggc tgacgaaaaa gttacttttg ctcagtttat
     5701 ggagcaaaag ggtctcccgg tcgtaccatc ctggcgggtg aatacgctgg cagaattaaa
     5761 gacacacctc gcggccccgc cgttcactga cagcccggta tgcgtgaagc cggtgacggg
     5821 tatctatggc atgggattct ggcgctttga tgacagtgct tcgcctatgg ccgtctttaa
     5881 tcatcccgaa catcgtctgg tcagtccgca acagtatatt gcagcagcat cagctgctga
     5941 gtcgtttaaa ccccttgttt tgatgccgta cctgccaggc ccggaatttt ccgtcgatat
     6001 cctcgcggat aagggcgaaa tactcgcagc cgtgggacgc cgtaaggaag gggctatcca
     6061 gtatctggta aacgaaggaa gcgcctggga actggcgtgt gactgcgccc gtgttatgaa
     6121 ggccgacggg ctggtgaatg ttcagacgcg aaacgatgtg aatggcaacc cggtgctgct
     6181 tgaaaccaac atgcgtccgt cagggggggt gggttatacc cttcacagcg gtgtgaacct
     6241 tcctgggtta tttgctgcct ttaagctcgg tctgatgtct gaagatatgg tacgccagag
     6301 cgctaaaaac accttttctc cggttgcggt gagatccatt acggatgtaa ttgcataccc
     6361 ggaatcactc tctaaccttc tgaattaagg cgtatttatg tctgatatcc tcgaagatat
     6421 gatttatcgc cgtaccctct catgcggtac gattcaggta acccgtgacc agggtgaagt
     6481 ttcgctcgat gacctgtttg acatcgctga aagacgtaac ccgaaacgcg cctttctgtt
     6541 tgtcagcaaa gtactcggca ggcacattcc cgtgccaccc tcagtcatgc gacaggccta
     6601 cagacagctt gccagccagt tcccctcgac actgacagga cccgtactgt ttatcggtat
     6661 ggctgaaacc gccgttgggc ttggggccgg tgtatttgat gaagtgcgcc accagcatcg
     6721 tgaatctgtc tatctgacct ctacccgtca cccggttgat ggcacgttgc tttgcgagtt
     6781 caaggaagaa cacagccacg ccaccgatca tctgatctat ctgccagatg atgaagagaa
     6841 aagacgtcgt gttaccaacg cacgaacgct ggttttgatt gatgacgagg caaccaccgg
     6901 taataccttt attaacctgc tttctgccct gcgtaatacc ggcaagcttc aacatattga
     6961 acaggttatc gccgtaacgc tgaccgactg gagtggcaat gccctgtcgg agcgcacccc
     7021 cttgcccgtc acttcggttt cgcttgtaag cggtcagtgg ggatggaccc cattacctga
     7081 tgcacctgtc ccggacatgc cgaaagtcaa cgtaacttca cgaggagaat gggacatcca
     7141 ggggaaacag tcatggggcc gactggggat gctcgcacct gcggccgatc tcggccatga
     7201 ggtctccgtc cacaaggggg aacgtattct ggttctcggg accggggaat tcgtctggga
     7261 gccgttcctg cttgctgaac ggctcgaagc tgccggagca caggcatttt atggatcgac
     7321 cacccgctcc cctgtcgccg ttggttatgc cattgagtcc gccatttcct ttacggataa
     7381 ctacgggctg ggcatcccca attttgtcta taacgtcgcc caccagcagt ttgaccgcat
     7441 tcttgtgtgt actgagacac ccgcagaaag tattgacacg cagcttctta aagcgctggc
     7501 tgaggttgcg cccgtcgtgg agattgttac ctatgaataa acccgttgtt ctgagcgacc
     7561 ttgacgacac gctctttcag acccgtcgta aaatggtgga cgagctggcc ctggagccat
     7621 tccgtaccgg tgccgttgat cgcaccctga atccacgaag ctttatgacg gaagaacagt
     7681 ccatgttggt ggactggctc ctggagcagg ctgaactcat acccgttacc gcacggggaa
     7741 ctgaagaaat cagccgcgta cggatccctt tccactcctg ggcaatcaca acgcacgggg
     7801 ccgtcattct cacgcctgaa ggcaaacccg acgaggagtg gaaggcccat atgctcggcc
     7861 agctggctcc ctaccaggaa aagctgacat cgatgcagcg tctgatcact gaaatgatgg
     7921 acgcaaaagg aatcaatgcc tgggcaaggc tgaacttcga atacggtgaa acggcggttt
     7981 atatggtgat gaaacaccgc gacagcaccc gccttgacga gctcaatgcc attgcagatg
     8041 agatagaaac ggtgtttccg accgagggct tctacatcca ccgcaacagt aataacgtgg
     8101 cctggcttcc caccccggtt gagaaagggc tggcagtcag ctggctgctt gaaaaacttc
     8161 gggctgaacg aggagttttc cccgtaattg gtttgggcga cagcctgagc gatcatcgtt
     8221 ttatgaaact gtgcagctgg tttggcatcc cgcgtcagag tcagtttgca gatgccattt
     8281 cacagcgaat ttttggagaa aattaaaatg cagccacatg aaacatttac cggctcatac
     8341 cagcccggtg acgtggaatt tctgctaaag ccggttgtca ttgagatgac gccggttgag
     8401 aaaaaagaag aactgattca gtccgggaag aaacattatt cggacatgct cagccaggag
     8461 ccagcgccta cacaatggca tcttgatttg tttcaccggg cgctggatcg gggcgcagag
     8521 aggcttgcaa aagaagtcac acagctcgct attgctctgg ccgaacgctt cggtgatgag
     8581 cccattgtac tggccagtct tgtcagggct ggcgttccgc tcggcgttat gctgcaccag
     8641 gccctgcgtg acatggggaa aacctcatgg cattacggta tcagcattat ccgggatcgt
     8701 ggaattgacg gtgcagccct cgacgtcatt gaagagcggc atggtaccag cggtattgtc
     8761 tttgttgacg gatggacagg taaaggcgca attaccggag agcttgtacg cgcactgaaa
     8821 gatcgcccgg gctatcctga gcagccgcga ctggttgtcc tggccgatcc ctgtggctgc
     8881 tcctggcttg cagccagtga tgatgactgg ctcattcctt ttggcatcat gggggcgccg
     8941 gtatcaggcc tgatctcccg gtcagtatgg tcctctgaag gattacacgg gtgcatggtt
     9001 tgcgagcatc tcagtgaatt cgaatgcagc cggatgcttg ccgataccgt cgctcatttc
     9061 cgtaagaagt taacaccgtc atccctcgct cccctgagct ggaatacgga atcagcccgg
     9121 gtcttatggc agacaagtcg cgacgttatc gcgttcctgg ccgatgaatt taaagtggac
     9181 agcgtcaatc gtattaaacc cggtattgct gaagcaaccc gggctgtatt acgtcgggta
     9241 ccggaccatg tatttgtgcg ttctattgac gacccggacg ttgccttgct tgtagggctt
     9301 gctcgtgaaa agggaatagt tgttacagaa atggggggaa ccctcggcca gtatcgggct
     9361 gtaaccatta tcaagaaggt actctgatga aaaatcgtct ttctccctgg aatctgggag
     9421 ccacgctata catgcctgca acacgggaag atattgctga tgccgtcctg cacgggaaaa
     9481 tcccgggctt gcgctctctg gtgatttgcc tggaggatgc tgtcagtgaa gcagacatcc
     9541 ccgtcgccct caaaaatctg gagcacctgc tgcacgagct cagtaacagc atgagtagcc
     9601 tgggtaaaaa tgactggcct ctggtgttca ttcgcccccg gcatgccgaa atgggcagat
     9661 ggttaaaagc gcattatgat ctttccgctg tcgacgggtt tgtgctgcct aaatttaccc
     9721 tcagttcgtt agctgaatgg tgggatatca tggtcgggac tcacctgtgc atgatgccaa
     9781 cgctggaaac agaagatgtc tttgacgtgg ttcagatgcg cgagctggct acccgtctgg
     9841 aggaacatcc ctgccacgac cgcattattg cgcttcgaat cggcggcaac gatcttatga
     9901 acgttgtgtc gcttcgccgt ccccgggacc tgacgctgta tgacagcccg atgggctacg
     9961 tcatcaaaat gctcgtttcg gtcttcggcc cgcgtgattt tgccctgacc gcgcctgtat
    10021 gtgagcatat tgacgatcat gccgttatgg ccagagagct ggctcttgat atggcacacg
    10081 ggcttgttgg gaaaacggcc attcacccgg gtcagataga ggtcattcaa aacgcgctga
    10141 tggtcactca gggtgagcat tctgacgctc tgcggatcct gaactctacc caggccgtgt
    10201 ttaagtcgca gggagcaatg tgtgaacccg ccacacatcg ccgctgggcg gctggcattc
    10261 tggacagggc tcgtttttat gggttacaga acgagcaaag cgctgatgga atcagattac
    10321 ttaccgtgac ccagcatcat taaggaaatc ataatggggt tcaggttcag aaagtcgatc
    10381 aacattattc ctggcgttcg cctcaacctg agtaacggtg caccgagcct gagtgtcggg
    10441 ccgagaggtg cttccgtttc ttttggtagc cgggggacct atgccaatct gggcttgccc
    10501 ggtaccgggc tgagttaccg tacccggctt gaccgggccg cgcgttccag aggtgaaaac
    10561 cggacggcaa ccgacccggg gctcagacag gcgcttgagc agaaagccgc tgaactcatg
    10621 tcagcggtaa ccgcaatccg taatattcac gagctgacgc cggatccaaa aacaggcatc
    10681 agcagggcag agctggaagc agtatacctg cataacagaa cgtcgccttt tcaggttccg
    10741 gcaccggtgc gtccagaaaa gccagactac cttgtattgc cggaaaagcc tgccgagagc
    10801 gaaggcatta gttttttggg taaatggttt gaatcggaat cagctaaagc tgagcgccac
    10861 gccgaaaatc ttcgccggtg gcagcaggag ctgattgatg tggagcgtga gaataccctt
    10921 cgacagcacc ggtaccagca acaacggacg gcctgggccg aacagtatgc aaactggaag
    10981 tttgaagctg aagaacatga aaaacggctc gccacggctc aggcagatgc ccggcagcag
    11041 ttccggacag acgccgcgtt tttcgaatca tacctggcgg gtgtgctggc agaaactgaa
    11101 tggccgcgtg aaacgcttgt tgcatttgaa gtaaagccgg agctatcagc agtcctgctg
    11161 gacgttgatt tagctgagat tgaagatttc cctgataaga tttacggcgt taatgcccgg
    11221 ggaacggagc tgacggaaaa agccatgacg caaaaagccg tacgcgaaaa ctatgcccac
    11281 cacgtccatg gctgcttgtt ccgcctggtc ggtatcgttt tacatacgct acctttcgac
    11341 aacgtgattg tgtcaggctt tacgcaacgg gtcagtaagc ggaccggcta tctggaggat
    11401 gagtatatcc tgtcctgcaa atgcactcgc agccagatgt cgtcagtaaa ttttgcaggc
    11461 ataaaacaca ttgatccggt tgaagcgtta ggcgatcaac cggttattcg aaagatgagc
    11521 agtaccttca tttttcagcc tattgaacca ctaacccttt aagcaggcta aatcttacga
    11581 tgcaattaaa taccagacag gcccggattt ttaagcttgc caacttactg ggcaccggca
    11641 agcccgtttc cgctgccgac attataacca gtctggaatg ctctgagccg acattaaccc
    11701 gggccctcaa agagctacgc gagtcatatt ctgcggagat caaatacagc aaggcaggtc
    11761 attcctatca tctcgttaat ccgggccagt tggataaaaa gaccctccgc cggatgaatg
    11821 aagcgctcgc acagaatgct gaactgaaaa ccggtgagtc cacagggaag gtcgtactcg
    11881 ataaggataa aaaaacagcc gtgtcattat cgctacgcat gcggattcta agaaaaattg
    11941 accgtctggc tgcgctgagt gactcgaccc gaagtgaagc ggtagagaag ctggctctgc
    12001 actccgttga tgaactgata aaagaataca gcgctaaaaa gtcctgacgc taaatctcag
    12061 accggggtga aaactccggt tttgtcatat ttcacctctg tattggcgtg atgcaaaatg
    12121 gcatctgaat cgatcatggt ttggcatcca tttccgatca cagtttggca tcacaatcga
    12181 tcacgatttg gcatgcttcc gctcattgat tggcatcctg gtgtcgttac ggggaagtca
    12241 tcttcacagg gatcttcagt cagttagcat taccgacatc cattcaccaa cttaccggag
    12301 ataatcaatg tcaaaacccc gatggacgct tgatcagaaa aaacatcacg ttgccgcatg
    12361 gcgcgccagc ggccttaccc gtgaacagta ctgtgaactt tacgacattc cgtttaaatc
    12421 cctgcgccag tggccgcagg atgtcgcaaa agccgaaaaa cgagccagag caccagaaat
    12481 tattcctgtt tcagtatctg gctcttccgg aatgactgat ggcagaccat tgtcggatga
    12541 gcctgtcact ctcttcctgc ccggtggtat ccggatgtgc tgccagcctt ctcagttaac
    12601 cgatgttttc cgggctcttc gccatgctga cgcctgataa cgtattcctg gtcgtaaaac
    12661 ccgtcgatat gcgccggggt atcgacacac tgacacagta cgtccagaac gaacttaacg
    12721 ctgcatggca tgacggtgct gcttttgtct tcaccaataa agtccgctcc cgcataaaag
    12781 ttctccggtg ggacaaacat ggcgtatggc tgtgtacccg acgactgcac cggggaagct
    12841 tccgctggcc ccggaaaggt gatgccacat ggcatctcac acaggatgag tttcactggc
    12901 tggtctttgg ggttgactgg caacaggtga aagggcatga cctggcaaaa tgggtatacc
    12961 aggaagaacc cgaagatgct cagagcaacg caataactca atgaaatata atgaatatcc
    13021 atgcgtgatg tcatataatc caccgcatgg atatctcgct tctcagtacc accagtgacc
    13081 ctgaacagct tcgcgctctg gcaattgcca tggtgcaaaa agtcatggct gagaatgcag
    13141 aacttcagaa ccgtatccgc atcctggaag agcagatgaa gctcgcacgc cagcagcgtt
    13201 tcggtaaaaa atgcgaatcg ctggccggga tgcagcgttc cctgttcgaa gaggatgttg
    13261 acgcggatat cgcagaaatc tcggcgcatc tggacaaact gcttcctcag accggggacg
    13321 aagaaaaaac aacaacacgt cctgttcgta agcccttgcc ttcacctctt ccccgtgctg
    13381 aaaaggttat cccgccagct gaggaacgtt gtcctgactg tgatgccccc ctgcacttca
    13441 ttcgcgatga agtcagcgag aagctggaat atattccggc acaggtcgtg gtcaaccgct
    13501 atatccgccc gcagtacagc tgtccctgct gtgagaaagt gttcagcgga aaaatgccgg
    13561 cacatatcct tccgaaaagt gctgttgagc catcggtcat cgcacaggtt gtcatcagca
    13621 aatataccga ccatctgccc ctgtatcgcc agcagcacat tttctcccga atgggggtgg
    13681 agctccccgt cagtacaatg gcggatatgg tcggtgttgc tggcgctgca ctggccccgc
    13741 tggcaaaatt gttacgccac gagcttctga cacgggatgt tattcatgca gatgaaacgt
    13801 ccctgaggct ccttgatacc cggaaaggcg gtaaatcctg ctccggctgg ctgtgggctt
    13861 acgtcagcgg agaaagagta agcgtcaacg gagcaccgta ttgacgctta tttattggtg
    13921 agtactacgt tccatggcag gagttcatca actcggttgg aaggccattc tggcagtacg
    13981 ctcaggatat ggcgcagata cgcttccgga tcgataccgt tcagacggca ggtgccgatc
    14041 agcccgtaca acagtgctcc acgctcgccg ccgtgatcgc tgccaaagaa cataaagttt
    14101 ttctttccga gacagactgc acgaagcgct ctttccgcag cattattatc cgcctccgcc
    14161 agaccgtcat cactgtaata acagagggca tcccactgat tcagtacata gctgcacgct
    14221 tcgcccagtc tggatttttt cgacagcgta ccattcttct ccaccatcca ttcatgcagt
    14281 gacgtcagta acactttgct tcgctgctgc ctgacggcaa gacgctctga ctccggtaat
    14341 ccccgtattt catcctcgat ggcgtacagt tcactgattc gcttcagggc ttcttctgcc
    14401 gtcgcacttt tgctgctgat gtatacatcg tggatttttc gccgggcatg ggcccagcac
    14461 gcaacttctg tcagtgcacc accttcacgt tctgcactga acagccgatc gtaaccgctg
    14521 aacgcatccg cctgcaggat accccggaag ggacgaaggt gttgtaccgg atgttttccc
    14581 tgcctgtctg gtgagtaggc gaaccagacc gccggtggct ctgatgagcc cgcattccgg
    14641 tcatcccgga catacgtcca gatgcgtcct gtttttgcct tttttctgcc cggtgccagc
    14701 acttttactg gtgtgtcgtc agtgtgaacc ttgcgggtgt tcatcacgta acggtacagg
    14761 gtatcattca gcagagtcat taactgacaa catgcgtcaa cccagttgga gagtaatgcc
    14821 cggctcagtt cgatgccctg gcgggcaaag atttcactct gacgatacag tggcaggtgt
    14881 tcgcagtatt ttcccgttaa cacgcgggca agtaatccgg agcccgcgat gccgcgctct
    14941 atcgggcggg acggcgctgg cgcttcaact atacagtcac attttgtaca ggcttttttt
    15001 acccgaacag tgcggatcac tttcagggcg ctactcacca gttccagctg ctcagcactg
    15061 acttccccca gataatccag ctcactgcca cactccgggc aacaactttc ttcaggctcc
    15121 agacggtgta tttcacgggg aagatgtgcc ggtaacggac gacgatgacg tgactgtcgc
    15181 aactggcggg gaacctgcgg gtcatcctca cgcccactgt aacgatcgct ttcctgttcg
    15241 cgttgtttca gttgggcctc agcctgttca acctcacgct gcagtttttc agaacgggta
    15301 ccgaacaaca tccggcgcag tttttctatc tgggccctca gatgttctat ttcccgctcc
    15361 tcctcttcga tcttttcctc ggcacgtgcc agtgcagagc gcaggaaggc ctccgtctct
    15421 tcaaccagac tcagttgctg atctttctga cggagggctt cagcctgctc agagagcagc
    15481 ctttccagct cagtgatacg aatgaggtat ttccgactca tgaccgtttt tataatccgg
    15541 ccatgacatt tttacaacat tgtcagtgca ttaaggcggg atgttttggg ctgacgccag
    15601 tccagtttat cgaggagcat tgccagctgc gagcgggtaa tggatacctt accgtcacgc
    15661 accgcaggcc agataaactg gccttcctcc agacgtttgg tgaacaggca cagaccatca
    15721 gcatcagccc acagaatttt aatcgtgtca ccccgtcggc cgcgaaagat aaacaggtga
    15781 ccggagaagg ggttctcatc cagcacatgt tgtacctgtt ctcccagacc gttgaaggat
    15841 ttacgcatat cagtaacgcc ggcaaccagc cagattcgag tgcctgatgg gagcgagatc
    15901 atcgtcccct cccggtcagt tcacggatca acaccgtgag cagctctggt gaaggatttt
    15961 ccagcgtcat gttaccgtgg cggaactcaa ctttacagga actggcactg actgtgcttt
    16021 gtgaaggagt ggataaaagc ggagtaagag ccgccatagg ctctttctgc tcatcaggcg
    16081 ttatctcaac aggtaataat tcaacgccag cgccagaaga ggttgttacc ggaagacgcc
    16141 gcgatatacg cctttcgttc tgccagagcc tgagccattt gaacaggagg ttatcattga
    16201 tatcgtgttc ccgggcaata cgggcaacag aggctcctgg ttgtgaagcc agtttaacca
    16261 tttgaagttt aaactcattt gaaaatgatc tgcggggttc tgaggataat attttctgtt
    16321 ccataacagg tgtccactag ttgaaaaggt gggcacctac gttaccaata ctggcttaat
    16381 ggctacatac ggcggtcagt ttacgcttac gagaaagaag cggtccgccg gtcgtctgtt
    16441 ttgactcgca gaccggacgg gccctcagat atcctgaggc ctggctgcag ggctggtgtg
    16501 gcggtacgct ggtgagtgac ggatacagcg tgtacaaatc tctggcggat aatgaccccg
    16561 gtattacatc tgcctgttgc tggagccacg caaggcgcgg tttcgcaaat ctgtataagg
    16621 ccagcaggga gccccgtgca gccatggcgc tgagaaaaat cgcgggtctt tatcgcattg
    16681 agaagttcat ccgtgaacgt cctgtggaaa aaatacggca atggcgacaa cggtattccc
    16741 ggccgatagt gaatgacctg tttgcctggc ctgaagagca ggagccgtgc tgtccgcccg
    16801 acggaccact aaacaaagcg attaattata tcctgaaccg gcgcgatgaa ctgagctgct
    16861 tcctgagtga cggagccgtg cccctggaca acaatatctg cgagcgtgct atccgcccgg
    16921 tggtcatggg gcggaaagca tggttattcg ccggttcact gatggccggg aaccgcgcgg
    16981 cacagataat gagccttctg gaaacggcaa aacgtaatgg tctggagccg catgcctggc
    17041 tgacagacgt tctgacgcgt ctaccggagt ggccggaaga ccgactggaa gagttgctgc
    17101 cccttgaggg atttaccttc tccgggtgag tgatacctgc catcaggtgt tcgtgcagcg
    17161 ggccataacc cgcagtcggg agttgaacgc ctgacgacag gaaatgagcc agagcagcga
    17221 ccacgcgggc tgacagtgag tcagaacctg aatcagcccg gagccattcc cggggctgta
    17281 cttcatcacc tgccatcagt acccgtgtca gtacttcgcg acctgacgca attacccgac
    17341 tcagttcttc agaacccgcc tccgatacca gcctggcggt cgccccggcg caacagaata
    17401 aatcaaccat cgtcagactg cgatcattcg tatgcatcgc tgatgatgcg cccgaaaaac
    17461 gcgtcaatat gagttcgccg gaatattact gacgacacgg tttgtagtta cgttccttcg
    17521 gtgagtgagt acctgtgcgt cagggtaacg caatcagttc aggtttagcg gggtgtcaag
    17581 tcggtttggc taaccgaccg cagaattaac tgtaccttta aaggtcgccc ccgatatatc
    17641 tgcctgtcag acatatcgcc gattcggtat ctcactacat aaaactcctt aaaaaacaat
    17701 gcgattctgt tggtacgaag ctttcagtaa gtcagtccgg agaagcgcca gtacgataca
    17761 gaatgaggtt gaaaaaaacg ttcacgtctg gtcacttctt caaatgttaa aaaacatctc
    17821 gtaaagagcc agtttatgcg ggttcgttga gtagacgtgc gagcgtcact ttgtcagctt
    17881 taacgctgac gaaagtgacg cccgcacagc ttcaaactcc acatgcacac gcaagcgacc
    17941 aaatttcaaa tggtcttatg ccgtgcgcac agtcataaga agcgcatgcg gaaagtcaga
    18001 aaatatctta agacgcagat tttaaaggcg tttttatgcc agtctgccgg gaatacacag
    18061 tagtccggtt gtccggaagc accacggtaa tccgaaggaa aaatcaacgt acctcaatca
    18121 ccgtagtgat atccgttccc agaggctaaa cggatgtacc accataaagg catcctcttt
    18181 caggctacga ggatattgca atcacccgaa ggcgtttctc tcagattgct gaaacattgg
    18241 ctggcagtca cccgaaggcg tccctctcag actgctggaa cattggctgg tcgcaaaagc
    18301 gacagttaat gcaaatacag gttaacaaaa agggaaatct gccgtcaacc caaccatatg
    18361 aacatgttga tttcattatg aatgtgatgt tctaatataa attaatcagt aacttcataa
    18421 aaagaatata agaaacagat aatgcatgta tttacacaca tatcagcaca atgatagatt
    18481 accaccccag aaacacgata aaggtataat tatggaattt tccgttattt ttttctgggt
    18541 tatcactggt cttattacca tcttattctt aaaatcaata ataaccgatc taataaaaaa
    18601 cc
//
LOCUS       DAZFDP010000092        18579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFDP010000092 DAZFDP010000000
VERSION     DAZFDP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            163..1383
                     /locus_tag="ACHJ1G_003234"
     CDS             163..1383
                     /locus_tag="ACHJ1G_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4765034.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVTLPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1356..1985
                     /locus_tag="ACHJ1G_003235"
     CDS             1356..1985
                     /locus_tag="ACHJ1G_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4765035.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1986..3146)
                     /gene="ybdL"
                     /locus_tag="ACHJ1G_003236"
     CDS             complement(1986..3146)
                     /gene="ybdL"
                     /locus_tag="ACHJ1G_003236"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4765036.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3255..4343
                     /gene="hcxA"
                     /locus_tag="ACHJ1G_003237"
     CDS             3255..4343
                     /gene="hcxA"
                     /locus_tag="ACHJ1G_003237"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4765037.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4353..4550)
                     /locus_tag="ACHJ1G_003238"
     CDS             complement(4353..4550)
                     /locus_tag="ACHJ1G_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4765038.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4732..6837)
                     /gene="cstA"
                     /locus_tag="ACHJ1G_003239"
     CDS             complement(4732..6837)
                     /gene="cstA"
                     /locus_tag="ACHJ1G_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4765039.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7018..7431)
                     /gene="entH"
                     /locus_tag="ACHJ1G_003240"
     CDS             complement(7018..7431)
                     /gene="entH"
                     /locus_tag="ACHJ1G_003240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4765040.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7434..8180)
                     /gene="entA"
                     /locus_tag="ACHJ1G_003241"
     CDS             complement(7434..8180)
                     /gene="entA"
                     /locus_tag="ACHJ1G_003241"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4765041.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8180..9037)
                     /gene="entB"
                     /locus_tag="ACHJ1G_003242"
     CDS             complement(8180..9037)
                     /gene="entB"
                     /locus_tag="ACHJ1G_003242"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4765042.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9051..10661)
                     /gene="entE"
                     /locus_tag="ACHJ1G_003243"
     CDS             complement(9051..10661)
                     /gene="entE"
                     /locus_tag="ACHJ1G_003243"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4765043.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10671..11846)
                     /gene="entC"
                     /locus_tag="ACHJ1G_003244"
     CDS             complement(10671..11846)
                     /gene="entC"
                     /locus_tag="ACHJ1G_003244"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4765044.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12215..13171
                     /gene="fepB"
                     /locus_tag="ACHJ1G_003245"
     CDS             12215..13171
                     /gene="fepB"
                     /locus_tag="ACHJ1G_003245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4765045.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHJ1G_003246"
     CDS             complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHJ1G_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4765046.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14536..15540
                     /gene="fepD"
                     /locus_tag="ACHJ1G_003247"
     CDS             14536..15540
                     /gene="fepD"
                     /locus_tag="ACHJ1G_003247"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4765047.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15537..16529
                     /gene="fepG"
                     /locus_tag="ACHJ1G_003248"
     CDS             15537..16529
                     /gene="fepG"
                     /locus_tag="ACHJ1G_003248"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4765048.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16526..17341
                     /gene="fepC"
                     /locus_tag="ACHJ1G_003249"
     CDS             16526..17341
                     /gene="fepC"
                     /locus_tag="ACHJ1G_003249"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4765049.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ1G_003250"
     CDS             complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ1G_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4765050.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4379 a         5253 c         4792 g         4155 t
ORIGIN      
        1 aaatgagagt ttttgatata taactacaga cagctactat aactttcatc tatttattca
       61 cagcgcgaat gaaaaataaa acactctgct ttgcagtgtt tactttataa ttaacagact
      121 tatctcactg atcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt
      181 cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac actcaacacc
      241 ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg
      301 acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggag
      361 gcgcaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc
      421 atcgaagagt tttactgggt tacgctcccg cttacgacgc aaaattccct ttcacaatac
      481 caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat
      541 gcgataaccg atcctgactt tttctgcttt taccagccag gcatgacctt cgaacaattt
      601 gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc
      661 cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt
      721 gccgacgata aaccatggac tacggccgca ccaggcggtc atagctggta catttacccc
      781 atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca aaatctctgc
      841 aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc
      901 gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat cgaaccggat
      961 cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga
     1021 catgataatc atttctatgg gcatcggaaa atcctcaagc cagaacattt agactggcaa
     1081 gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat
     1141 aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag
     1201 caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc
     1261 aataacgatt actggtgtcg ggcattatca tttagtccta cgaaagcgaa gaactatcag
     1321 cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat
     1381 taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaaagcga
     1441 ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt
     1501 gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg
     1561 caccacccga aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca
     1621 ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg
     1681 aaattggtaa aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct
     1741 gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg
     1801 gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga acttagccag ttgggatggg
     1861 acgataataa aattggcaaa gagctaggaa tggacagcga cgaagtgctg cgcctgaagc
     1921 aaattaacgg cctgcaagag ctgtttgccg accgtcaata ttcccgcgcc tggacggtta
     1981 aataactaaa gctggcgcag gcgttcagct gctgccagca acgtcgattc cttcttggca
     2041 aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga cagcggaatc
     2101 gccgctacgc cgtgctcctg cgtcagccac tggcaaaact caacatcatc cagggtagaa
     2161 acctcgctgt aatccaccag caaaaagtat gtaccttcac acggtaaaat ctccagccgg
     2221 ctttcattta aagcattcac cagaatatcg cgcttctggc gataaaagtc cggtaacgca
     2281 agataatgct caggttctgc acgtagcata tcagcaagcg ccagctgtgc cggggtattc
     2341 accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca
     2401 acacaataac ccactttcca gccggtcata tgataggtct tgccaaatga agaaaccgcc
     2461 actgcccgct cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag
     2521 ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc
     2581 cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tggggttatg cggagtgttg
     2641 aggatcacca gtctggtgcg ctcgcttaat aatgcggcaa attcctgcca gtcaacgcga
     2701 aaatgcggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     2761 gagtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     2821 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     2881 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     2941 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3001 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3061 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     3121 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     3181 cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     3241 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     3301 tttcacatcc aggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc
     3361 gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     3421 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     3481 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     3541 gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgccg tttgttgccg
     3601 ttccgacgat cgccgccacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     3661 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     3721 cggagattat cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg
     3781 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     3841 gactggggat caataatgcg caggccattc gcgacgtatt gttaaacagt agcgaacagg
     3901 cgctgagcga tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg gtggatgcta
     3961 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg
     4021 ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaaattt ctccacggca
     4081 ccaaagtcgc ttatggcatt ctggtacaaa gcgccttgct gggtcaggat gatgtgctgg
     4141 cgcaattaac cggggcgtat cagcgttttc atctgccgac cacgctggcg gagctggaag
     4201 tggatatcaa taatcatgcg gagatcgaca aagtgattac ccacaccctg cgtccggtgg
     4261 aatccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     4321 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     4381 ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg
     4441 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     4501 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     4561 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     4621 tgctatcaac gcgctcggtc agtcccctcg ccccatcggg gagagggtta gggtgaggag
     4681 gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac tttagtgtgc
     4741 gccttttgcc tgtgccacga tctcctcgac attttccggc attggctcat acggcgtttc
     4801 tttcgccgtt ggcttcggat ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc
     4861 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     4921 caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
     4981 tgcctggaac ttattagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5041 ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     5101 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     5161 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatgc cgcccagcgg
     5221 atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ctgttgccag
     5281 caggttagca ggcagtgaat cggtccgctt caggcccgga gaaaccacgc ccagcagatc
     5341 ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc
     5401 ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc
     5461 gtgcaggatg taagccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat
     5521 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc
     5581 ccagctactc accacctgcg cggcagaagc gactacatcc gccgtccctg ccggagccag
     5641 caccgccatt gggctgttca tggcaaaata cacgcccgga tcgatgatac aggcagaaac
     5701 cagcgccata atcgccacga aggattccat caacatccca ccgtagccga taaagcacgc
     5761 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     5821 agacaccgca ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     5881 cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat
     5941 caaaatgcct accgccagac cgacgatagt cccgattttc aggaaggtcg agagatagtc
     6001 acgcggggcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt agcccaccag
     6061 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     6121 cacccagccg ccagagataa tggcgaaaat caggaacacc aggccaatca ctgacacttc
     6181 gccaatgcgg cccggacgca gatagcgcag gtagattccc ataaaaatcg ccagcggaat
     6241 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     6301 cgccagtact gcaaggataa tgaccatgat cataaagcag gctaccagcg caatcacccc
     6361 ggcagttggc cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt
     6421 agaaacaaac agcaccatga aatcctgcac cgcaccagcg agaaccaccc cggcgagcag
     6481 ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccaa ccaacggacc
     6541 tgctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     6601 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac
     6661 atttttggcg atatacagcc cataaaaacg gtaagcgatc agatagatac agaccgacgc
     6721 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
     6781 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     6841 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtaag
     6901 atttaacaac acggtaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     6961 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
     7021 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7081 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     7141 cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtaccca ccacacactg
     7201 cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     7261 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
     7321 ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     7381 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     7441 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     7501 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     7561 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     7621 acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc
     7681 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     7741 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     7801 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     7861 gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc
     7921 gcgcccatgc gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct
     7981 aacagtcgct gacatacctg cgcgacctgc gcagcatcgg caacatccat cacttcggtc
     8041 gcaaagggat attgctcctg agcgaacgct tgatcaaaac cggtaacttt cgctcccgcc
     8101 tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca
     8161 tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga
     8221 tggtcgggtt tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg
     8281 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     8341 acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg
     8401 cggggacagg tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca
     8461 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     8521 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     8581 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     8641 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     8701 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     8761 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaaccg
     8821 gaatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     8881 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     8941 acgcggcacg ttgcggttca aaggcccagt caactttatt cttagggata tcgtgagact
     9001 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9061 gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc
     9121 ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
     9181 cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcgcag
     9241 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcgtagat caccgccggg
     9301 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcaccgcc acggttgatc
     9361 tgatctttct cgcgcccctg cacggtgatg tacccctctg gatcaataga gatcagatcg
     9421 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa
     9481 tagccacgga aggtgtacgg accgcgggtc atcaggcgtc caacttcccc ttgcggcagt
     9541 ggatttcctt cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg
     9601 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
     9661 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg
     9721 gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
     9781 tcaccttcgg tcagcgcctg caaccacagg ctgactgccg gtggcaccag cgcggtgacg
     9841 ttcacctgat gtttttcaat caatgggaag caaagcgtgg cgctgggatc ggccgccaga
     9901 acaaccgttc ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta
     9961 tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca aatctcgacg
    10021 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg
    10081 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgg cgtggcagta
    10141 aaatcctcag ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    10201 agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg
    10261 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    10321 gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac gcccagtttc
    10381 agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt
    10441 tcaccaggtt taatgccctg acggcgtaaa ctacacgcga ggttatccgc cgcctgattc
    10501 agctcccggt aactcaactg tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg
    10561 tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    10621 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    10681 ccaaaaacgt tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    10741 gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    10801 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    10861 ccgccaaaca gttcgcggtc gaatggctcc agttcagcaa taacctgggt cgcggcctga
    10921 tgcgggaaac cgctcagcgc gggggttgga tgcagcagac aggccagagt cagtgcgttt
    10981 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11041 atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    11101 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    11161 cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    11221 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg
    11281 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    11341 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac
    11401 accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca
    11461 aacgtggttt gttcaggaat tgcctggcgt tccaccacgt tcagcgactg gctgcgggta
    11521 aaacggcggg ctgaggtctg tttttcctgg cgggagaacg actgccagga ttcgggaata
    11581 tacagcgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    11641 atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    11701 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    11761 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    11821 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    11881 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    11941 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    12001 agcattattg atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc tgccccctct
    12061 cccttccagg gtgagggctg gggtgagggt taatgttcgc accagtgctg gctgttcccc
    12121 tcaccctctc cccaaagggg cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac
    12181 gcccataacc ttattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa
    12241 cgcccttcta ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgctgactg
    12301 gccgcgtcag attactgaca gccgtggcac acatacactg gaaagccagc cgcagcgtat
    12361 tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc
    12421 cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg
    12481 gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcaccga
    12541 agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc
    12601 gctggcactg tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga
    12661 caaaagctgg cagtcgctgt taacgcagct tggcgaaatt accgggcatg agaaacaagc
    12721 ggcagagcgg attgcacagt ttgataagca actggcagcg gcgaaagagc aaatcaaatt
    12781 accgccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg
    12841 gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt
    12901 gcccgcaggg ttaaacgcca gccaaagcca gggcaaacgc catgacatca ttcagcttgg
    12961 tggggaaaat ctggctgctg ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca
    13021 gaaagatgcc gatgcgattt atgccaatcc actgctcgcg caccttcccg cagtacaaaa
    13081 caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccacgca
    13141 agtgctggaa cgacttaagg cgctgtttta agcattaact gtcagacgct gtcacctgcg
    13201 gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa
    13261 tcaacaaacc aaaaccgctt gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac
    13321 cacccagcag cgccgcgcct atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt
    13381 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg
    13441 aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    13501 gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata
    13561 accctggtcg cgcactatgt gccagcttcc cgctggttaa cgcaccaata gccgcgccga
    13621 gcgggatcgc cgcgtagaga aaaccaatct gtgccgctga catctgccag ttgtcagcca
    13681 gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa
    13741 tcccgcccac cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg
    13801 gatgctcacg cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg
    13861 taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca
    13921 ataaaccgcc aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    13981 ccccggcctg cattaagttt tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg
    14041 taacgccaag cgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg
    14101 acggctccgg cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc
    14161 gcgccagcaa aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccaaccatca
    14221 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat
    14281 gcgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg
    14341 agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    14401 taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    14461 ttcttaggct gcacatgcta acatatccaa ataagataga taacgataat taatttcatt
    14521 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    14581 ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc
    14641 cccgcttccg tagtgctgga ggccctctcc ggcacctgcc agagcgccga ctgcaccatc
    14701 gtgctcgacg cgcgactgcc gcgcaccctt gccggtttac ttgcaggcgg cgcgcttggc
    14761 cttgccgggg cgttgatgca aaccctcacc cgaaatccac ttgccgaccc cggcttgctt
    14821 ggcgtgaatg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca
    14881 tccgcgcagg aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt
    14941 gcctttaccg gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc
    15001 gtggcgctgg cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgat
    15061 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc
    15121 ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt actgagccgc
    15181 gcgctgaaca gtttaagcct cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg
    15241 cgcacacagt taattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta
    15301 gttggcccga ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt
    15361 gccgatcatc gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt
    15421 gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg
    15481 tttattggtg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga
    15541 tttacgtctc tcgccgatta atcatcacct gtttgctgtt ggtttccgcc tgtgtggttg
    15601 caggtatctg gggattacgc agcggtgccg tcacgctgga aacctcgcag gtattcgccg
    15661 cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    15721 gcgtgctgat ggcgctgttg attggcgcgg cactgggcgt cagcggcgcg atttttcagt
    15781 cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga
    15841 gcggcgtgct ggtagcgatg gtgctgtttg gtcaggacct gacggctatc gcgctggcag
    15901 caatggtggg cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggca
    15961 tcgacacctt tcggttgatt attatcggta tcggcgttcg tgccatgctg gtggccttta
    16021 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcgggt ttgtggaatg
    16081 caggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat
    16141 tgatgctcat tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg
    16201 ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg
    16261 cagtggtgct taccgctgcg gcaaccgcgc ttgccgggcc gatttccttt attgctttag
    16321 tcgcgccgca cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg
    16381 cgctgtgcgg ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc
    16441 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct
    16501 tgttaattca ggagtctcgc aaaaaatgaa cgaatctgta gcccgtttgc gcggcgaaca
    16561 gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga
    16621 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccactt tactgcgtac
    16681 tttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca
    16741 acattacgcc agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac
    16801 gccaggcgat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc atcaaccgct
    16861 atttacccgc tggcgcaaag aagatgaaga agcggtaacg aaagcgatgc aggccacggg
    16921 aataactcat ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgcgc
    16981 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac
    17041 ctggctggat atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga
    17101 gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag
    17161 ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt
    17221 cactgctgaa ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt
    17281 agccggaacg ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta
    17341 aactaagtgg tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa
    17401 cacgctacca caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt
    17461 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    17521 attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa
    17581 ttcaccgttc agttccgcaa cgtcagtgac cgctttttca atttccagtt tgcgttcaat
    17641 accgtctgcg ccgagagaaa tagagaaatc ggggtcatct ttaacggcct gaccattact
    17701 gtatacgggc tttttaattc ccgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg
    17761 aatgtttgca tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc
    17821 aaactgggtt ttgatttcca gtttattacg gacgttttct agtgactctt tcaccaccaa
    17881 cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc
    17941 ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt
    18001 gtcatcaacc gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc
    18061 atcgattttc gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc
    18121 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    18181 tttgatatcc agatccagca ctcgaagctt agtgaatgtt ttctccagct cttgccactg
    18241 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    18301 gatcagcaag cctgcgcagg caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg
    18361 ccataaaact gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    18421 atactcagga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    18481 cctgaaaatg agtccaatag gggaaatctc cacggcgctc actttaccag ttggtgataa
    18541 ttatccgaag ctgaagtttg taaattcctt ccactgaat
//
LOCUS       DAZFDP010000093        18347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFDP010000093 DAZFDP010000000
VERSION     DAZFDP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1113
                     /locus_tag="ACHJ1G_003251"
     CDS             <1..1113
                     /locus_tag="ACHJ1G_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4765051.1"
                     /translation="NMQRLPGVQADMEIVPGEQPGESDILITRKQDKYWRLGFSLDDS
                     GTRSTGRYLGGITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQF
                     SVTASDYDYHQTVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRN
                     YINDTEIQVQRRQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEA
                     TALSKILQMNASFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDG
                     ERTLNASNGWYIRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRG
                     QLFSTDYDVFIGRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            1123..1650
                     /locus_tag="ACHJ1G_003252"
     CDS             1123..1650
                     /locus_tag="ACHJ1G_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO4765052.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARFLTQPAILMPQSDWNSGDR
                     MWVCDWIAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            1666..10983
                     /locus_tag="ACHJ1G_003253"
                     /pseudo
     CDS             1666..10983
                     /locus_tag="ACHJ1G_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538208.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            10980..11465
                     /locus_tag="ACHJ1G_003254"
     CDS             10980..11465
                     /locus_tag="ACHJ1G_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765053.1"
                     /translation="MINVNSIAKDIEGLESYLTNGYVEANSFNDPEDDALECLSNLLV
                     KDSEGGLSFCKKILNSNNIDGVFIKGSALNFLLLSEQWSYAFEYLTSNADNITLAELE
                     KALFYFYCAKNETDPYPVPEGLFKKLMKRYEELKNDPDAKFYHLHETYNDFSKAYPLN
                     N"
     gene            11725..12366
                     /locus_tag="ACHJ1G_003255"
     CDS             11725..12366
                     /locus_tag="ACHJ1G_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO4765054.1"
                     /translation="MLQTEKAREHGADVLSCSDNPSGEACKRGQAENKAYAAALATGS
                     VALLPGSAQAMWLLGVGTNAGMQYADSGEINPVNSVAAGWINVITMGQGWKGTIAWNA
                     AGGALTNAINGDDPLTGAITNGAGAGFGYGVGNYVVKPAANTIGKWITGGWNPKFDPT
                     LLKYTEVKGQLGLSKEMVPSKIPGAVGNAGGSLSSEFGSSIIQKKKDAMEAGK"
     gene            12654..13040
                     /locus_tag="ACHJ1G_003256"
     CDS             12654..13040
                     /locus_tag="ACHJ1G_003256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765055.1"
                     /translation="MTVCRKAGGDCGDIQNKYAVLNAESRHQLHLDVAADPLTALSGE
                     DKWNIEGGLSAAGRLGWLYGSLTNQEVKDYVAEGNSYDLNYLNSNTSKGDRALAFFGE
                     PENYWGTVAGAGSLLISSAHADGKIC"
     gene            13299..13856
                     /locus_tag="ACHJ1G_003257"
     CDS             13299..13856
                     /locus_tag="ACHJ1G_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4765056.1"
                     /translation="MKTLSVISAIILSVASGSVLADNHSFSVGYAQSKIQNFKNIRGV
                     NLQYRYEWDSPVSIMGSFSWMKGDGNQEYHSYGDSIKNQVDAKYYSLLTGPVWRINDY
                     MSLYAAGGVAHVKADGKTAWTNVDGYFARENISKKSTSFAYAAGVMFNPASNITINAG
                     YEGTRAKLGDNYSINGFNIGVGYCF"
     gene            complement(13892..14637)
                     /locus_tag="ACHJ1G_003258"
                     /pseudo
     CDS             complement(13892..14637)
                     /locus_tag="ACHJ1G_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15275..17749)
                     /locus_tag="ACHJ1G_003259"
     CDS             complement(15275..17749)
                     /locus_tag="ACHJ1G_003259"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4765057.1"
                     /translation="MSLDFDKLNAGIGAKQTDPRKIFTTLKRDARFKRPLDEQADVLD
                     VWYARRQAQDLTLKMNTGGGKTVVGLLCLQSALNENVKPAVYITPDSFLVNQVLDEAK
                     ALGIPATENEKDPDFLAGRAILVANVWKLFNARSVFGVGQKKIPIGCVVVDDAHACLS
                     VVHDQFSIKADVGTPVYDDLLNLFKNDLDKQSPTGLLDIQALDPSIVMEVPYWAWQSK
                     QKEVIALLHAQRNSAELEWSWPLLRDCIPLCTCLFGGGKLEIAPYFIPIDRIPSFATA
                     QRRIYMTATLADDTILVSHFGALADEVVKPIRPKGGGDIGDRMILAPQEINPDFKMEE
                     IRELVSDIAKDLNVVVIVPSKERAKFWKDSANQILNKDNIQAGIQRLKDGFVGLTVLI
                     NKYDGIDLPGKACELLVIDGLPEVYGLSERREMLLLDGTKRQLVRQVQRIEQGMGRGV
                     RSSDDHCVVLLLGGRLTQRLHQPEAETMFSSATRAQIELGKSVAEQLRGQSLEKLQEI
                     MGLCLTQDDNWVQAGRNAVVNAPPVEGGRIDDNQVLLREAFDAARVSRYDTAKNKAQT
                     AVNAASERQIKGYLKQQLAAYTNFTDPSQAQELQLAALSDNYRLLKPLAGTTYHRLSA
                     PASGQAAAAVQFMTRFLEGNDLIIWVNGLLDDLQWGEEGSKRFEAAIKELGIFLGFGS
                     ERPEDLVGRGPDNLWALGNSRYFVIECKSGAVLAERISKHDTNQLNGSIVWFDEKYGH
                     TCTRTPILVHPKTIFEHAASPHSDIRIVNEQGLNRMRDAIQAYSISLASNGGYADSQI
                     VHRQLKHHKLSAEDIEDLCTVAQGAK"
     gene            17913..18338
                     /locus_tag="ACHJ1G_003260"
                     /pseudo
     CDS             17913..18338
                     /locus_tag="ACHJ1G_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT         4660 a         4314 c         5214 g         4159 t
ORIGIN      
        1 aatatgcaac gactgccggg tgtgcaggct gatatggaaa ttgtgcccgg tgagcagcca
       61 ggggaaagtg atattcttat tacccgtaaa caggataaat actggcgact ggggttttcg
      121 cttgatgatt caggtacccg cagcacaggg cgttatctcg gtggtataac attttctctg
      181 gataatcctt tttccctgag tgacctgctg tatgtttctg ccacccataa ttttccacat
      241 tacgggggta agggcagtaa aaactacacg gcccattact ctgtgccttt cggttactgg
      301 cagttttccg tcaccgccag tgattacgat taccatcaga cggtagctgg tgcggttgag
      361 gattaccagt acagtggcga gagtcagaat ctgggcatgc agctgagccg ggtactgcac
      421 cgtaacggga cgcagaaaac ggtgctgact tacgatgttc aggcccgcag gtcgcgtaac
      481 tacattaatg atacggaaat ccaggtgcag cgccggcaga cagcggcctg gcgacttggg
      541 ctgcagcacc gtcattatat ttctcaggcc acactggatg ctggtgtcag ctggcaacgg
      601 ggaacccgct ggtttggtgc acagccggca ccggaggaaa tcttcggtga ggcaacggcg
      661 ctcagtaaga tcctgcagat gaatgcgtcc tttaatctgc cctttacact ggcagggcag
      721 cccttcagtt attccttccg gtatcagcgc cagctgagca ataccccgct gaccccacag
      781 gaacagtttg ccatcggtaa ccgctggacg gttcgtggtt ttgacgggga gcgaacgctg
      841 aatgccagta acggctggta tatccgcaac gacctggcct ggcgtacgcc actgccggag
      901 caggagttat atctgggaat ggattacggc gaggtggggg gagccggcag tgaatacctt
      961 gtgggacgtc atctggccgg tggtgttgtg gggctgaggg ggcaactgtt cagtacggat
     1021 tatgacgttt tcatcggtcg accgttttca aagccggcgg gctttatgac gagtgatgtg
     1081 acagccggtt ttaatctgtt ccgggatttc tgagaaagtt tcatgcgtaa cgggaaatat
     1141 gacgttctga gtcccctgta ttccggtgag ccggtcaatg aggcggaagt cctgggggcg
     1201 gccgtctggt tgtggatgca ctcccccctg catcgtgatg cgccactgca taccctgccg
     1261 gacctgctgc tgccggtgat taagcaccga cagtatgtgg tggcaacaga gcaggggcga
     1321 ccggttttct tcatgagcca ggcctggctc agcccggagg cagaggcgcg ttttcttacg
     1381 cagccggcaa ttcttatgcc tcagtcggac tggaacagtg gcgaccggat gtgggtctgt
     1441 gactggattg ccccttttgg acataccccg gatatgagtc gtcttgtccg gcaggatatt
     1501 tttccccgtc tgtgctggcg ttcgctgtac caccgtgggc agcagaccgg gttacgggtg
     1561 atgaatttcc ggggggcaca ggtcagtcgt gaagaggcgc gccagtggcg tgtgcagcac
     1621 ccgcttgctg tggatgtgcc ggagagataa gtcaggttta tcagaatgaa caaaaattgt
     1681 taccgcatta tttttaacaa agcccgggga atgctgatgg tggtggcaga tattgcccgt
     1741 tccggtcgcg ccggtgcatc accctcctcc cgtacaggat atccacgcag gcaacgtatc
     1801 tgccgtgtca catcgcttgc attctccctg tggctggcct cggggatggt gcattctgtc
     1861 agtgctgcag gcattgtcgc agaccatggc gcaccggggc atcagcagcc cacgattatg
     1921 cagacggcca gcggcattcc gcaggtgaat attcagacgc cttctgccgg cggggtttca
     1981 cataacacat acagccagtt cgacgtcggt aacaagggtg tcattctgaa taatgcccat
     2041 aacaatgtgc agactcagct gggtggcatg gtggccggga acccgtggct ggcgaaggga
     2101 gaggcccgaa tcattcttaa tgaagtgaac agtcgcaatc ccagccagtt gaacggtttt
     2161 gttgaggttg ccgggaaaaa ggcgcaggtg gtgattgcga acccggccgg catcagttgc
     2221 gacgggtgcg gttttattaa tgcgaaccgg gccacgctga ccacggggca acctcagatg
     2281 aaaaacggca gtctggccgg attcagtgtt gaacgaggtg agattcagat tacgggcaaa
     2341 gggatggatg cctcgcgcac ggattatacc gatatcattg cccggtctgt gaagattaat
     2401 gcgggtatct gggcgcagga cctgaaagtg accacaggac gtaacaatgt ggatatcgcg
     2461 cacgggcaga ttgagaaaaa gaccggagat gtctcttcac agcctcagat ggcactggat
     2521 gtggccagtc ttggcggaat gtatgccgga aaaattcgcc ttgtcggtac ggaaacgggg
     2581 gtgggggttc gtaacgccgg tcatattggt gcgcaggcag gggcggttac cctgacggct
     2641 gacggacgta ttgaaaacag tggcagtatc agtgcgaaaa cggatgtgca tctggcgaca
     2701 acccgggagc tgcataacag tggttctgtt tatgcggggc aggatacgca gatacagagt
     2761 gatggtgtat tcacccatac aggctctgtg gcatcacgtc gcaacacccg gattcagaca
     2821 gcgcgtctga ccggcggtga gcggagtctg ctggccgccg gggtgaaaga tgacggacgt
     2881 ctggcggcag cgggcaacct gactgtcagt accaccggcg aactggcggc acacggacag
     2941 gttctgtccg gtggggacat gcaactgaag gggcaggggc tggatttaag caacagccgg
     3001 atacagggac aacatactga gctggatgca ggcagtggaa atctgtccac acagaatgca
     3061 caactgagtg ccggtacgct ttccgcccgg actgccgggc agttcagcaa taatggtggc
     3121 acggtaaatg ccgacacact gcagatatcg gcacagagcc tgtcaaatcg taaaggcagt
     3181 ctgattcaga cgggaacagg ggatttttcg ctgagtctgc cgggacgcgt ggataaccgg
     3241 gaagggctgc ttgcggcaaa tggcgcggtg cgcctggatg cactgagcct tgataatcgc
     3301 agggggaaag tgcaggcggc gcagtcaggc agtctgcagg tgaagacaac cggtgccgtg
     3361 gataaccagc agggacgtct gcttgccagc cgtgacatcc tgattaatac acaggcgctg
     3421 aataacgaca acggactgat ttcagctgct gccggaacgg gcaggataaa aacacagcag
     3481 gctgtcagca atacggaggg acgaatggag tccgccggca ggctgggtat ttcggcaggg
     3541 agtctgaata accaccaggg tacggtggtg tctgacgggc tgtccgtgac gctggatggt
     3601 gcgctggata atacgtccgg cagactgctt tcacagaaaa cgctgtctgt gtcaggcagc
     3661 gaactggtct ctgatgatgg tctgattcag tccggcagtg acatgacgct ggatgtacag
     3721 aacggggttc tcagtaaccg gaacacgaaa acacggggtg gtatcagcag cgccggtacc
     3781 ctgacagtac gggccggtat gctgaataac cagcagggat ttatggcggg tcagaaggat
     3841 atgaccctga acgccggaac gcttgataac cgtcagggcg tgctggggag tcaggcgtca
     3901 ttacagatat cgtcaggtac gctgatgaat cagaaaggtg cactgaaggc cgggacggat
     3961 atgttgctga gtggtggcga tgtcagtaat caggaaggga cgcttgccgc cggcggggac
     4021 ctgaatgcgc acctgaatgt gctggaaaat cagcagggga cggtggtcag caacggtaac
     4081 agcaggctgg atgtcacccg ctctgataat cagggcggca gactggtggc acagcagtca
     4141 ctgacccttt cctcgacgga tatcagcaat gatgcaggtg gtctgataca gagcggtgcc
     4201 acactgaatc tccgtgctga cacactgagc aaccgtaaca gtggtgaccg gggcggggtt
     4261 atcagtcagg ggccgatgac gctgaatgcc ggcacactgg acagcactgc cggtgtgctt
     4321 ttgtcgggag atgctctgtc cctgactgca ggcgtggtta acaatacatc cggtcaggtg
     4381 gtggcaaacg gtctgctcgg ctggaacagc caggcgctga acaatcagtc aggtcttatt
     4441 caggggaaag gcatcagcat taataccgca ggccagacgc tggataacag aggcggtacg
     4501 ctgaacagcc tgcaggaact gactgtcagt accggagcaa tggataaccg gggcgggacc
     4561 gtgggggcga aaacgacagc tgacctgagc accacctcgc tggataaccg tgagggtggc
     4621 cggctggtca gtgaaggtga actccgtctg catacgggag gcctgcagaa cagccacgga
     4681 cagatacagt ctgtgggcga cattctgttc gacagtgtac ggggcgttgt ggacaacgtc
     4741 tctggtctga ttcgcagtgg ttctgccatt acgctgaacg ccctgcagtt tatcaatcgc
     4801 cacacgcaga ataccggcca ggggctggaa gcgcagacaa tacacatcac gacgcaggac
     4861 cttgataacc tggagggcag tattctggca gacagggcgc ttacggttat ggcagacagg
     4921 acgctcagta ataatgatgg cgtgctctct tccggtgcga cattgtctgt cagtggccgt
     4981 cagctgacgt tttcgaaccg tgacggcgtg gttaaggccg ggcagtcagt atctgtggat
     5041 gccggtcagc tcggtggtga cgggaaactg ctttcgctgg gcgatatgac gctgaaaagt
     5101 aacacaacgt tcagcaacag tggtcagact attgcgaacg gaaaccttac gctttccgtt
     5161 aatggcgatg tctctaatac cggcagcctg ctggcaggca gccggcttga cctgaacagc
     5221 atccgccttg aaaacacaga gaaaggtgaa atcagtgccg ggcagacctg gcttaacgtg
     5281 acggacacac tgctgaaccg tgggctgatt gacgggaaat atacgcgtct tcaggcgaac
     5341 accctgacga attccggtac cgggcgaatc tacggcgatg ctgttggtgt cagcgctgcc
     5401 acattgaata atctggatga aaatggcgtg gcggcgacac tggccgggcg tgagcgtgtg
     5461 gacctggggg tacagaccct gaataaccgg acgcacagcc tgatttacag tgcaggtgat
     5521 atgcatatcg gtggcacgct ggatgcgaat ggcaccgcca caggtaaagc cggtgtactg
     5581 aataatcaca gtgccactat tgaagccgcc ggatatctgg cactgagtgc cggacaaatc
     5641 aataacgtca atgaccattt caccacggag cgcgtggtgg tttccacgga aaaggtgact
     5701 gagtatcagc atcgcggatc agatgtccgc tggaatgcaa aagatgaggg ggtgttcatt
     5761 gataacaact ccactgatgg tctgctgaac ctgaacacgc cggatgatac cggacataac
     5821 aacgaccggt tcacaaaata tgaatacacc cgtaccgtgg aggaaacccg ggtaaaagac
     5881 tccgacccgg ggaaaatact gtccggtgcc ggcatgacgg ttgtggcaga caaactgctt
     5941 aatgacaaga gtcaggttgt tgcaggtggc ctgctggata tgcaggccgg tgatgtggaa
     6001 aacgtcagtg tcagcggaga gcgccatgtt actgataaag ggacatccac atattattac
     6061 cggataaaaa gaaaaaataa aaatgacagg cagggggaaa gtatttcccc ttacgagccg
     6121 cccaccgtca ttcagaccat cacgctgaag ccgggagagc tcaccagcca cggacaggtt
     6181 cagggcagcc aggtcacgct ttctcccctg aagccacagg gaacggatgt acaggcagga
     6241 ctgaccggaa atgtggatgc gacagtagcc gctactgacc ggataccgct tcgcccggtg
     6301 gtgtctgccg gtgaaccggt cattctgttg ccggggcagc agtttgaagt cagcgcacct
     6361 cagggcagta tccatgttgc cgggccggat acccgtctgc cggacagcag cctgtttaaa
     6421 acaaatccgg cagtgaatgt gccatatctg gttgaaacgg acccgcgttt tacgaaccag
     6481 aagacgtggc tgggcagtga ttacatgcag aaggcgtttt ctcagaacgg ggataacatg
     6541 ctcaaacgcc tgggggatgg tttttacgaa cagcgtctga ttcgtgagca ggttgtcgct
     6601 ctgaccgggc aacggtacct ggacggatac agtaatgatg aagagcagtt taaggcgtta
     6661 atggatgcgg gtattgcatt tggtaagcaa tacaacctga caccaggtgt ggcgctgact
     6721 gctgagcaga tggcgttact gaccggtgat attgtctggc tggttaacac cacggtgaca
     6781 ctgccggacg gcagcacgca gacggttcag gtacctcagg tgtatgcccg tgtaaaaccc
     6841 ggtgatgtga acagtgccgg tgcgctgatt gcgggtcggg acatggtgat gaagctggat
     6901 ggtgacctgt ttaacagcgg taaactggcc gggaagcaga ccgtgcagct cagtgcagaa
     6961 aacattcaca accaggccgg caccattcag ggagcaaatg tcagcctgac ggcccggacg
     7021 gatattaaca gtaccggcgg gctgctgcag gctacggaca gcctgctggc gatggccgga
     7081 cgggatatca gtctgaccac caccacccgt acggcacagc gcgatgccgg gcagaaccat
     7141 tttgaacgta ccagtatcga cagtgtggcc ggcgtgtatg tgcagaacga ccagggaaga
     7201 ctggtgctgc aggccggacg ggatatgaat ctgacggctg caacggtggt gaatcagggt
     7261 aaggacagcc tgacgcaact cagcgcgggc agggatatga cgctttccac agtgaccaca
     7321 tccgcgcagg acaagattac ctgggataaa aataatcgtc tgtcgcaggg cgtcacacag
     7381 agtacgggca gcacgcttgc cggtaacggt gatgtcacgc tgaccgccgg acgggatatg
     7441 acgtcactgg cggcatcgct gtcagctcag aaaggcctgg ccctgatggc gggtcatgac
     7501 gtgacgctga ccggtgcaca gaatacccgc tcgctggatg aataccataa agtgaccggc
     7561 agcagcggga tgttatcgaa aaccaccaca accacgcatg atgtcactga ccgccggacc
     7621 atgacgggca gtgaactgaa cggggatacg gtcagtatca gcgccggaca taatctgaac
     7681 gtcacgggca gcagtgtggc cggggataac cgtgtctccc tggctgccgg aaataacctg
     7741 aatataggta cgctgacaga aagcaaccgt gaaacacacc tgaaacagga gaaaaaatcc
     7801 gggctgatga gctccggagg tgtgggattc agcgtgggca gccagagtct gaaagtcacg
     7861 gacaccgcaa cagataccac gcagaaaggc agcacggtgg ggagtgttca cggtgatgtt
     7921 tcccttcagg caggtaaccg gttgaccgtc aacggctcag acctgattgc cggcagggac
     7981 atggcactca gtggtaagga ggtcagcatt accgccgcca cagaccagca tgtacagaca
     8041 catacggtgg agcagaaaac ctccggtctg acgcttgccc tgtccggcac agcgggcagc
     8101 gccctgaata cgacggtgga gaccgtacag gcggcgaaat ctgccggaaa cagccggctt
     8161 gaagccctgc agggggtgaa ggccgcgctc tcgggggctc aggccgtgca ggccggacga
     8221 ctggcagaag cccagggcaa ccagaaagag aataacaatg ccgtgggtat cagcctctct
     8281 tacggcagcc agtcttcaaa atcagaacag cagtcagagc agacggtggc gaagggcagt
     8341 acactgaccg caggtaataa cctcagtatt caggccactg gctccggcgt gaagggcgtg
     8401 gatggtgacc tgacgataca gggcggtcag ataaaggccg ggaacaacgt tctgctgcag
     8461 gcaaaccgtg acgtgaatct ggtatcagca gaaaacacgt caaagctgga aggaaagaac
     8521 acatccagtg gtggcagcgt gggtgtcggt gttggtgtgg gctccggagg ctggggtatc
     8581 agtgtttcag ccagcgcaaa ccagggtaag ggcagtgaaa aaggcaacgg taccacccat
     8641 acagaaacca cggtggatgc gggaaaccgg ctgaccatta tcagcgggcg ggacaccacg
     8701 cttaccggcg cgcaggctgg cggtgaaacg gtgaaagtgg atgccggtcg ccatctgaca
     8761 ctgaccagtg agcaggacag tgaccgttat gacagtaagc agcagaatgc cagtgccgga
     8821 ggcagcatcg gcaccggtag tgcctcagcc agtgtgagtc acagccggga taaaatgcac
     8881 agcaactatg acagcgtgca ggagcagaca gggatttttg ccggcagggg cgggtttgat
     8941 gtcaccaccg gacagcatac gcaactgaac ggtgcggtga ttgcgtccac ggcaacggca
     9001 gataaaaacc ggctggatac cggcacactg ggcttcagtg acattgaaaa ccgggccgac
     9061 tttaaaacgg aacatcagag tgcagggctc agtaccggtg gcagtgttgc cggaaatttc
     9121 ctggggaaca tggcaaacaa tctgctggtc ggtgcaaatc atgaaggcca tgcggacagt
     9181 accacgcagt cggcagtgtc tgcagggaat atcaccatcc gggatacgca gagccagaaa
     9241 caggatgtgg cagacctgaa ccgtgacgca gcacatgcaa accagacgct gtcccccatc
     9301 tttgacaggg aaaaagagca gcagcgactg cagcaggcgc aactcattgg tgagattggt
     9361 aactaggtgg cagatattgc cagaacggaa ggacagatag ccggtgagaa agcgaagcgg
     9421 gacccggcag cactgaatca ggcccgcgca gagcttgagg cagcaggaaa accgttcacg
     9481 gaacaggatg tggcgcagcg ggcctacaat aacggtatgg cggcctccgg ttttggaacg
     9541 ggaggcaaat accagcaggc gatacaggcc gcgaccgcag cggtacaggg actggccggc
     9601 ggtaatctga gtgcagccct ggcaggtggt gcagcaccgt atctggctga agtggtgaaa
     9661 accatgacca cagacccggt tacaggtgag gtgaataagg ctgcaaatgt ggctgctcat
     9721 gctgtggtga atgccgcact ggcggtggca cagggtaata acgctcttgc cggtgccgca
     9781 ggggctgcca ccggcgaggt ggtgggaatg atagccacac agatgtacgg taagccggtg
     9841 agtgaactga gcgagacaga gaagcagacg gtctcgacac tggcaacggt tgctgccggc
     9901 ctggccggtg gtctggtggg agactcgggg gcttcagcgg tagccggcgc acagtcaggt
     9961 aagacgactg ttgagaataa ttatcttagc gtgtctgaaa agacagagct tgagatagca
    10021 aaacagacgc ttaaaaacag taaagatccg gcagagcgcg aaaaggcgca gcagaaatat
    10081 gatgcattgc ttgaaaaaga tatcagcagt gataaggcgg taatcgctgc atgcagcaac
    10141 ggacaggcag ccagcgcggc ttgtgcaggt gaaagactga gggttattgc tgctaaaggc
    10201 ggttatgaaa ccggaaacta caataaccaa gccagcgaca tgtatccgga tgcttacggg
    10261 cagatagtaa acctgctgaa tatcaccagt gttgatgcac aaaatcaaca acaggtgaag
    10321 gatgcgatgg tgaactatgc catggggcag tttggggtcg atagggcgac ggcacaggca
    10381 tacgttgaaa cttatgatgg aatgaaggtt gtagctgctt cgatggcacc agtaataggt
    10441 gctgcggcgg ccagtaagat agaagttctg gctggtaaac aacgcttgtc taatagtttc
    10501 gaggttagct cacttccaga tgcaaatggg aaaaatcaca tcacggctgt aaaaggtgat
    10561 gctaaaatac ctgtcgataa gattgagcta tatatgcgtg gtaaagcttc tggtgacctt
    10621 gattctttgc aaacagaata taattcttta aaagatgcaa ggattagcag ccaaaaagaa
    10681 tttgcaaaag atccaaataa tgctaaaagg atggaagtat tagaaaaaca aatccataat
    10741 attgagcgtt ctcaagatat ggctcgtgta ttagagcaag caggtatagt gaatactgcc
    10801 agtaataaca gcatgataat ggataagtta ttggactcag ctcagggggt tacgtctgca
    10861 aatagaaaaa catctgttgt tgtatcaggg cctaacggta acgtaaggat ttatgctaca
    10921 tggacaatat tacctgatgg cacgaaaaga ctttcaactg ttaatacagg ggcattcaaa
    10981 tgataaatgt gaatagtatc gcaaaggata ttgaaggttt agaaagttac ctgactaatg
    11041 gctatgtaga ggctaactct tttaatgacc cagaggacga tgcgttagag tgtctcagta
    11101 atttattagt taaagattcg gagggagggc tgtcattttg taaaaaaata cttaattcaa
    11161 ataacattga tggtgttttc attaaaggtt cggcgctgaa ttttttatta ttgtcagaac
    11221 agtggtctta cgcctttgag tatttgacaa gcaatgcgga taatataact cttgcagagc
    11281 ttgaaaaggc gcttttctat ttttactgtg caaaaaatga gacagaccct tatcctgtac
    11341 cagagggttt attcaaaaaa ctaatgaaaa gatatgaaga actgaaaaat gaccctgatg
    11401 caaaatttta tcatcttcat gaaacgtaca atgatttttc aaaagcttac cctctgaata
    11461 attagaagct atcgccgtgg aatattgtct atgactgcat ggaaagatga tggtcatagt
    11521 gtgttgggag ctattcttta acagacttgg gggaaatggt gtaactggct gaaagggcgc
    11581 gtcagggcaa aaagttgttg cgccggaatt acagcaggac aaagcggctt taactcgctg
    11641 aaagcacaga gaaaactggt tttatcacag caacctgcgg cggaaagagt tgttgttgag
    11701 aataacctgc tgggtggcag tgaattgctc cagacggaaa aagccaggga gcatggagct
    11761 gatgttctct cctgtagtga taatccttcc ggtgaagcct gtaagcgggg gcaggctgaa
    11821 aataaagctt atgcagccgc attagcaaca ggcagcgttg ccctgttacc ggggagtgcc
    11881 caggctatgt ggttacttgg tgtcgggaca aatgccggga tgcagtatgc agatagtggt
    11941 gaaataaacc cggtcaactc agtggcagca ggctggataa atgtgataac catggggcag
    12001 ggctggaaag ggacaatagc ctggaatgcg gcaggtggag cattaaccaa cgccataaac
    12061 ggagatgatc ctcttactgg ggcgattaca aatggagcgg gggccgggtt tggatatggg
    12121 gttggcaatt atgtggtcaa gcctgctgca aatacaatcg gtaaatggat aaccggtggc
    12181 tggaatccta aattcgatcc gactctacta aaatatactg aagttaaagg gcagttaggt
    12241 ctatctaaag aaatggtacc gagtaagatc cctggtgctg tgggtaatgc gggtggtagt
    12301 ttatcatctg agtttggaag ctccattatc cagaaaaaga aagatgcaat ggaggctggg
    12361 aaatgaagaa ccttcttatt gttgtcttat taatgattgt atgcatattt ggtttgttta
    12421 tagtcggttc gattttttat cttcttctgg agatatttat gtatttttat ttgaatgccc
    12481 caatatcatt tgaagttttt cagttttcta gactgttaaa aatgagtgtg tatggggggg
    12541 ggatcttagg cttgggtatt ggtttgttgc atatcatgaa agttaagggg ttctgaaatg
    12601 ttgttgagaa taactacctc agctacgacg agacgcatgc ttttgacaga gaaatgacgg
    12661 tctgtcgtaa agcgggcgga gactgtggcg atattcagaa taaatatgct gtattaaacg
    12721 cagagagccg acatcaatta caccttgatg tggccgcaga tcctcttacg gctctgtccg
    12781 gggaagataa atggaatatt gagggcgggc tcagcgcggc gggccgactt ggctggctgt
    12841 atggttcact gactaatcag gaggtgaagg attacgtggc tgaaggcaac agctacgacc
    12901 tgaactatct gaacagtaac acgtcgaagg gtgacagggc tctggctttc ttcggggaac
    12961 cggaaaatta ctggggtacg gttgccggag cgggttcgtt gttaatatca tcagcccacg
    13021 ctgacggaaa aatttgttag tgcggggctg agctatgggg ctaatggtgc ggtacaaatt
    13081 ggtacgggaa atatcggtga taagttcgat tacctcagtt tcatgatgtc aggcttgact
    13141 ggtgtaggta catccgggaa aggatattac gctaaccagt tgttgggtgc tggtagtgct
    13201 tacatgagca accagattga aggcctagac aatacgtatc taaaactatt agttttaatt
    13261 tcattaaaat aacgcttatt aacaatggag ataacgaagt gaaaacattg tctgttattt
    13321 cagcaattat attatctgtg gcaagtggct ccgtgctggc tgacaaccat tcattttctg
    13381 tcggttatgc acagagtaag attcagaact tcaagaatat cagaggggta aacctgcagt
    13441 acaggtatga atgggactcc ccggtcagta ttatgggcag tttctcttgg atgaaagggg
    13501 atggtaatca ggaatatcac tcatatggcg attcaattaa aaaccaggtt gatgcaaaat
    13561 attattcctt actgaccggc cccgtatggc gcattaacga ttatatgtca ctgtatgcgg
    13621 ctggcggcgt ggcgcatgtt aaagctgatg gtaaaacagc atggacaaac gttgatggtt
    13681 atttcgccag agaaaacatt tcgaaaaaat ccacatcttt tgcttatgct gccggggtaa
    13741 tgttcaatcc ggcaagtaat attacaatta atgccggata tgaaggaacc agagcaaaac
    13801 tgggcgataa ttattccatt aatggattca atattggcgt gggttattgc ttttgatgag
    13861 cctgtttacc attctgtgta aatgtctttt cttaaaagtg accgtccagg cggtcaccaa
    13921 actcgataat aaagcggctc attgccattc gccagtccct taacggcatt gcccatttct
    13981 gtgacgcggc ctggattgcc agccacgcca cttttttcac cgagtcgtct gtcgggaaca
    14041 ctttacgttt tttgatcgca tggcggatca cgctgtttag cgactcgatg gcattcgtcg
    14101 tatagatcac tttgcggatg tccgtcggat aagcgaagaa cgtggcaaga ttcggccagt
    14161 tagcctgcca gcttcggctt atctgaggat agcgactgtc ccagaccaca gcgaacgctt
    14221 ccagtgcctg cagacctgcc tcttatcagc gtcatacagc tctttgaacg cggcgactat
    14281 ctcacgggtg gtcatccctt tggcatacaa cgagaggatt tggttatcca tcccggtaat
    14341 acggggctga tttttcttca ccagttgtgg ttcgaaggta ccgtcacgat cgcgcggagt
    14401 acgcagttcc agtggaccgt cgcctgtgat aacggtcttt gtggaataac cattgcggga
    14461 gttagttccc agtctggact gatttttctc atacccgaca tgatgagtca tctctgcatt
    14521 gagagtggct tcaacgctga tctttttcag caaacggtca agctggctga ggtcttcagg
    14581 ggttttgagg tttttggcca gttcgttagc cagagcctgt aactgttttt cgtccataaa
    14641 ttaaccttca tttgatgctg gattgaacat atcaagatca ggcaattaca caaatctatg
    14701 tacaggctcg gggaattggg catctctacc gcttacgtcg agccgctcac acccggtcaa
    14761 tgacctgaca atctcccggc aagtcccgac gtcgggactt gcgcccctga caccgaaaaa
    14821 tagcaggaac ccgctcgcta cgggtcctca gctcagtgca aacctgactc agctcctgca
    14881 ttacatcccc gaaattacca cactcagtac ttcatcacct gccaccaata cccgtgtcag
    14941 tccttcgtga cctgacgcaa ttacctgact cagagcttca gagtcagccg ccggtacctg
    15001 cccggcgctc gcccctgcgc aaccaagata aatcgaccat tggcagactg cgatcgttcg
    15061 tatgcatcgc tgatgacgcg cccgaaaaac gcgtcaatat gagttcgccg gaacgattct
    15121 gtcaaacttt acagtttaac tatcttcgcc aactctgccc gaatgcaaga actacttgca
    15181 tgcagcttac cagttaaggc tagttgacga cggctagcaa gctaaattta atcgtatgtc
    15241 accaatcaac aaatgcgggc ccaagtgagt tacgtcactt tgcaccttgt gctaccgtac
    15301 acaaatcctc aatatcttca gcagaaagtt tatgatgttt aagttgccta tgaacgattt
    15361 gcgagtcggc atacccgcca ttactcgcca acgagattga atatgcttgt atcgcatcgc
    15421 gcatcctatt gagtccttgt tcgttaacta ttcggatgtc ggaatggggc gatgccgcgt
    15481 gttcaaatat ggttttagga tgcaccaaga tgggagtcct tgtacaagtg tgaccgtact
    15541 tttcatcaaa ccagacgatg gacccattta actggttcgt gtcatgtttg gagattcttt
    15601 cagccaaaac agctccgctc ttgcactcaa tcacgaaata cctcgagttg ccaagagccc
    15661 aaagattatc tggtcctctc cccactaggt cttctggcct ctcggatccg aaaccgagaa
    15721 aaatgcctaa ttccttaatg gcagcttcaa accgcttcga cccttcttct ccccattgta
    15781 gatcgtctaa caacccgttg acccatatga tgagatcgtt tccttcaaga aaacgtgtca
    15841 tgaactgaac ggctgcggcc gcttgtccgc tagcgggtgc gctgagacgg tgataagtcg
    15901 ttcctgccaa gggtttaaga agtcgatagt tatcgctgag cgcggccagt tgcagttcct
    15961 gcgcctgtga agggtcagtg aaattcgtat acgccgctaa ctgttgcttc agatagcctt
    16021 tgatctgacg ctcgcttgct gcattcactg ctgtctgagc cttattctta gcagtgtcat
    16081 atcgcgacac ccgggccgca tcgaaggcct cccgcaacag aacctgatta tcgtcaatcc
    16141 gtcccccttc gacaggtggc gcattcacaa ccgcattacg gccagcctgt acccagttgt
    16201 catcttgggt caggcatagg cccataatct cctgcagctt ctcaagcgat tgtccacgca
    16261 actgctccgc tacactttta ccgagctcaa tctgagcacg agtggctgaa gaaaacatcg
    16321 tttcagcctc gggttgatgg agtcgttgcg tcaacctgcc accaagtaat agtacgacac
    16381 aatgatcgtc actagaacgt acaccacgac ccattccctg ttcaattctc tgaacttggc
    16441 gcacaagttg gcgtttggtt ccgtctagca gcaacatttc acgcctttca ctgagtccgt
    16501 aaacctccgg caagccatca atgacgagca actcacaagc ctttcctggc aaatcgatac
    16561 cgtcgtattt attaattagt acggttaggc ctacaaaacc gtccttgaga cgctgtattc
    16621 cagcctgaat gttatctttg ttaagtatct gattagcaga atctttccag aattttgcgc
    16681 gttctttaga ggggacgata acaactacgt tcagatcctt tgcgatatct gacacaagtt
    16741 cgcggatctc ctccattttg aagtccggat tgatctcctg tggggctaga atcattcggt
    16801 ctccaatatc tccgccccct ttaggtcgga tcggcttaac tacctcgtcc gccaatgccc
    16861 caaagtgact cacgaggatc gtatcgtcag ctaaggtggc cgtcatatag attcttcgct
    16921 gagcagttgc gaaggatgga atgcggtcga tcggaatgaa atacggcgcg atttctagtt
    16981 taccgccgcc gaaaaggcag gtgcagagcg ggatacaatc gcgcaacaag ggccaactcc
    17041 attccaactc tgcagaattc cgttgtgcat gaagcaacgc gattacttcc ttttgtttag
    17101 actgccaagc ccagtatggc acttccatta caatgctagg atctagagcc tgtatgtcga
    17161 gcaatccagt gggggactgt ttatccaagt cgttcttaaa aaggttcaga aggtcgtcgt
    17221 acacaggtgt gccaacatct gcctttatcg agaactggtc gtggactacg ctaagacatg
    17281 catgtgcatc atcgacaaca acacagccta tcgggatctt tttctgtccg accccaaaaa
    17341 ccgatcttgc attgaatagc ttccacacat tggcaacaag aatggcacgt ccagcaagga
    17401 aatctggatc cttctcattt tctgttgcgg gaatgccgag cgccttagcc tcatctagta
    17461 cctgattgac aaggaaacta tcgggcgtaa tgtatacagc gggcttgacg ttttcgttga
    17521 gggcgctctg caagcacagc aggcccacca cagtcttacc accgccggta ttcatcttca
    17581 gcgtcaaatc ttgggcctgc ctccgtgcat accaaacgtc aagtacgtcc gcttgttcat
    17641 ccaatggccg cttgaaacga gcgtctcgct taagcgtcgt aaagattttt ctcgggtcag
    17701 tttgttttgc accgatacca gcgttcagct tgtcaaagtc taggctcatg taataatctc
    17761 aagttaagat tgggctatgt aagtccccaa tatttctata atgtattaca atgaccatgc
    17821 aacgagatag tcattgcccc aattagaact ttaagacact ctgtttctag attcattgaa
    17881 ccactacaaa ttttgttaaa actctacaag attgttacga taattccatt cattataccg
    17941 acagtttgtt gggacaggtt ttcgctatgc tggaaacgcg ccgggcatcc gtcatgtatt
    18001 tttcagagca cggtctggag tatgatccga caaaagaaca tgcctatttt catggcggaa
    18061 taaaacccag ccagcaggct tatcatgttc cgatgtttat ctggtacagc ccgacattgg
    18121 gagagcatgt cgatcgccag acggtgaaca gcgttttttc cactgcgtat aatgattatc
    18181 tgattaatgc atggatgggc gtaaccagac cggctcaacc gaaaacgccg gaggaggtga
    18241 ttacccgctg gcaggggaaa tcagtggttt ttgatgccag tcataacatg tttgactaca
    18301 aagtattacg aaagaacttt aatgaaaccc tggagtaatt cattaaa
//
LOCUS       DAZFDP010000094        18336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFDP010000094 DAZFDP010000000
VERSION     DAZFDP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..548
                     /locus_tag="ACHJ1G_003261"
     CDS             <1..548
                     /locus_tag="ACHJ1G_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4765058.1"
                     /translation="RHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTV
                     TLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYS
                     VSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEI
                     YGMWIDDMNDEQIAMLNARLS"
     gene            600..1535
                     /gene="ttcA"
                     /locus_tag="ACHJ1G_003262"
     CDS             600..1535
                     /gene="ttcA"
                     /locus_tag="ACHJ1G_003262"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4765059.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1664..3037)
                     /gene="dbpA"
                     /locus_tag="ACHJ1G_003263"
     CDS             complement(1664..3037)
                     /gene="dbpA"
                     /locus_tag="ACHJ1G_003263"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4765060.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3067..3240)
                     /gene="ynaL"
                     /locus_tag="ACHJ1G_003264"
     CDS             complement(3067..3240)
                     /gene="ynaL"
                     /locus_tag="ACHJ1G_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4765061.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3515..4498)
                     /gene="zntB"
                     /locus_tag="ACHJ1G_003265"
     CDS             complement(3515..4498)
                     /gene="zntB"
                     /locus_tag="ACHJ1G_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4765062.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4512..4559)
                     /gene="ydaN"
                     /locus_tag="ACHJ1G_003266"
     CDS             complement(4512..4559)
                     /gene="ydaN"
                     /locus_tag="ACHJ1G_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4765063.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4753..5985
                     /gene="dgcM"
                     /locus_tag="ACHJ1G_003267"
     CDS             4753..5985
                     /gene="dgcM"
                     /locus_tag="ACHJ1G_003267"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4765064.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6006..6569)
                     /gene="smrA"
                     /locus_tag="ACHJ1G_003268"
     CDS             complement(6006..6569)
                     /gene="smrA"
                     /locus_tag="ACHJ1G_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4765065.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6899..7807)
                     /gene="abgR"
                     /locus_tag="ACHJ1G_003269"
     CDS             complement(6899..7807)
                     /gene="abgR"
                     /locus_tag="ACHJ1G_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4765066.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7983..9293
                     /gene="abgA"
                     /locus_tag="ACHJ1G_003270"
     CDS             7983..9293
                     /gene="abgA"
                     /locus_tag="ACHJ1G_003270"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4765067.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9293..10738
                     /gene="abgB"
                     /locus_tag="ACHJ1G_003271"
     CDS             9293..10738
                     /gene="abgB"
                     /locus_tag="ACHJ1G_003271"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4765068.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10775..12301
                     /gene="abgT"
                     /locus_tag="ACHJ1G_003272"
     CDS             10775..12301
                     /gene="abgT"
                     /locus_tag="ACHJ1G_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4765069.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            12312..12827
                     /gene="ogt"
                     /locus_tag="ACHJ1G_003273"
     CDS             12312..12827
                     /gene="ogt"
                     /locus_tag="ACHJ1G_003273"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4765070.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13022..13774
                     /gene="fnr"
                     /locus_tag="ACHJ1G_003274"
     CDS             13022..13774
                     /gene="fnr"
                     /locus_tag="ACHJ1G_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4765071.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            13926..14876
                     /gene="uspE"
                     /locus_tag="ACHJ1G_003275"
     CDS             13926..14876
                     /gene="uspE"
                     /locus_tag="ACHJ1G_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4765072.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(14926..15183)
                     /gene="ynaJ"
                     /locus_tag="ACHJ1G_003276"
     CDS             complement(14926..15183)
                     /gene="ynaJ"
                     /locus_tag="ACHJ1G_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4765073.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            15427..16458
                     /gene="ynaI"
                     /locus_tag="ACHJ1G_003277"
     CDS             15427..16458
                     /gene="ynaI"
                     /locus_tag="ACHJ1G_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4765074.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(16509..18122)
                     /gene="mppA"
                     /locus_tag="ACHJ1G_003278"
     CDS             complement(16509..18122)
                     /gene="mppA"
                     /locus_tag="ACHJ1G_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4765075.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         4661 a         4549 c         4456 g         4670 t
ORIGIN      
        1 ttcgtcacgg ggaactggca gctctggcgt gggaggatgt ggatctcgaa aagggaatag
       61 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
      121 ggatccggac agtaacacta ctgcagcctg ctcttgaagc actgaaggag caatacaaac
      181 tgaccgggca tcatcgcaaa agcgaaatca ccttttatca tcgggagtac ggcagaaccg
      241 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacggaaaa caaaaacctt
      301 attactcggt aagcagtttg ggggcaaggt ggaatgcagc agtaaaacgt gctggtattc
      361 gccgccgtaa tccgtaccat acgcggcata cttttgcctg ctggctgttg acggcaggag
      421 cgaacccggc atttatagcc agccaaatgg ggcatgaaac tgcgcagatg gtgtatgaaa
      481 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      541 tatcatagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      601 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      661 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      721 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      781 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      841 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      901 agattgttga agagaatact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      961 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
     1021 gggcgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
     1081 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1141 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1201 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1261 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1321 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1381 attttaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1441 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1501 atgagttacg actgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
     1561 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1621 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1681 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1741 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1801 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1861 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1921 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1981 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     2041 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2101 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtagca accagtacac
     2161 gggcgctacc gttagcaaaa cgtaccagag tctgatcgcg atcgcgttgc tccagatcgc
     2221 cgtgtaacga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2281 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2341 gcaacagagg gattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2401 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgtactcgt ccgctgattg
     2461 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2521 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2581 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2641 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2701 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2761 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2821 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2881 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2941 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     3001 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3061 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3121 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3181 aacaggatcg ctaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3241 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3301 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3361 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3421 atggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3481 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3541 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3601 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaaggtac
     3661 tgggtaaaaa gaccattgcc atcaatgaca ttgtataggt acgacgagct aaattttcct
     3721 gcatcacctg agcgatttca tccgccatca caccagtccg tgctatacag gcgtcgattt
     3781 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3841 tcatccacgg caaacgttca ctggcaagac gagcatagac atcacgttgc ggtgccatat
     3901 agcgacgcat cacgattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3961 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     4021 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4081 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4141 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4201 gttcatcggt gctgccgttt atacagcgca atgtaataag cgtgccttca ccgagacggc
     4261 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4321 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4381 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4441 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4501 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4561 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4621 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4681 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4741 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4801 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4861 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
     4921 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4981 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
     5041 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
     5101 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5161 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5221 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5281 tgggaaatta atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5341 cccggcggtc ataaaccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
     5401 gtgcagacct atgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
     5461 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
     5521 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5581 cagcacctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5641 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5701 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5761 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgt
     5821 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgtt ttaccgtcag cattggtgtg
     5881 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5941 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     6001 cgagatcaac ggctgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6061 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
     6121 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6181 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6241 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6301 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6361 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6421 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6481 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6541 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6601 tgataaccag accgggtcgg tccaacaacg tcttacccaa aattccagta ataagttcca
     6661 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6721 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6781 ccgttataac acagtcaccg gcgcagagga gacaatgccg gacttaagac gcggatgcac
     6841 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6901 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6961 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     7021 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7081 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7141 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7201 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7261 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7321 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7381 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7441 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7501 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atttcctctt
     7561 gcgctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7621 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7681 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7741 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7801 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7861 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7921 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7981 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     8041 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8101 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8161 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8221 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcacg ggtatcgtcg
     8281 ccaccctgga taccggtcgc cccggtccgg ttatggcttt ccgtgtcgat atggacgcgc
     8341 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8401 gtaacgccgg aatgatgcat gcctgtggtc atgatgggca taccgccatt gggcttgggc
     8461 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8521 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8581 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8641 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc gggaccgccg
     8701 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcaa
     8761 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8821 ttatgcaggc aggaagcggc cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8881 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8941 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg cctgatgggt gcagctaccg
     9001 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9061 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9121 tgatggcccg cgtgcagcac catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9181 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9241 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9301 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9361 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9421 ggcttcggcg ctggaatctg caagcttcac cgttacccgc aacgtaggca atatcccaaa
     9481 tgcatttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9541 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9601 tgaaaatggt cacggttgcg gacacaattt gctgggaacc gccgcctttg ccgctgcaat
     9661 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9721 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9781 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggcatgttca atacccgcac
     9841 gctggcaaac attcaggcat catggtgctt taaagggatc gcagcacatg ccgcgaattc
     9901 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9961 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
    10021 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10081 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10141 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10201 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10261 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10321 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10381 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10441 tgcggcatcg actgatgtcg gcgacgtcag ttggaaactg cctgttgccc agtgtttcag
    10501 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10561 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10621 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagttacgga
    10681 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10741 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10801 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc
    10861 ttttttgctc tttatctatt tgataatcgt actcatggtg acgacggcaa ttttgtcagc
    10921 ctttggcgtc agtgcgaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct
    10981 cagtgtggaa ggattacact ggtttttacc caatgtgatt aaaaacttta gcggttttgc
    11041 tccacttgga gcgatcctgg cgctggtttt aggtgccggc ctagcggagc gcgtcggctt
    11101 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11161 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc
    11221 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11281 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11341 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11401 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11461 cttgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11521 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11581 gctacttttt attgctgcga tcgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11641 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactaatcat
    11701 tctttttttc tttgtggttt cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11761 ggcggattta ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat
    11821 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat
    11881 cgccgtgggg ctgaccgata tacttgaaag ttcagggctt agcggcatcc cggcgtttgt
    11941 cggtctggcg ttgctttcct ctttcttatg catgtttatt gccagcggtt ccgcaatctg
    12001 gtcgattctg gcccccattt tcgtaccaat gtttatgcta cttggctttc acccggcatt
    12061 tgcgcaaatc ctctttcgta ttgccgactc atccgtattg cctttagcgc cggtatctcc
    12121 ttttgttcca ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac
    12181 ttactattcg ttagtcttgc cctatccgct tatctttttg gtggtatggc tgctgatgtt
    12241 gctggcgtgg tatcttgttg gtctgccgat aggtccgggg atttacccac gtttgtctta
    12301 agagagaacg gatgctgaga ttacttgaag aaaaaattgc cacgccactg ggtccactgt
    12361 gggtgatgtg cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac
    12421 gcatggtgca gctgctggac atccattatc gtaaagaagg ctatgagcgc atttctgcca
    12481 ccaacccagg cggtttaagc gacaagcttc gtgattattt tgccggtaat cttagcatta
    12541 ttgatacgct tcccactgcc acagggggga cgccatttca gcgcgaagtc tggaaaactc
    12601 tacgcactat cccctgcggg caggtaatgc attacggcca actggctgaa caattgggcc
    12661 gccctggcgc ggcgcgtgcc gtaggtgcgg caaatggatc gaatcccatc agcatcgtcg
    12721 taccttgcca tcgggttatt ggtcgaaacg gcaccatgac cggatatgca ggcggagttc
    12781 agcgaaaaga gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt
    12841 tgtgccagct tgttcacact tttatgtaaa gttaccctta acaacttaag ggttttcaaa
    12901 tagatagaca tatatttaca tctaatatcg gaattctctg ctgttaaggt ttgcttagac
    12961 ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc aattacggct tgagcagacc
    13021 tatgatcccg gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg ctatccattg
    13081 ccaggattgc agcatcagcc agctttgcat cccgttcaca ctcaacgaac atgagcttga
    13141 tcagcttgat aatatcattg agcggaagaa gcctattcag aaaggccaga cgctgtttaa
    13201 ggctggtgat gaacttaaat cgctttatgc catccgctcc ggtacgatta aaagttatac
    13261 catcactgag caaggcgacg agcaaatcac tggtttccat ttagctggcg acctggtggg
    13321 atttgacgcc atcggcagcg gccatcaccc gagcttcgcg caggcgctgg aaacctcgat
    13381 ggtatgtgaa atcccgttcg aaacgctgga cgatttgtcc ggtaaaatgc cgaatctgcg
    13441 tcagcagatg atgcgtctga tgagcggtga aatcaaaggc gatcaggaca tgatcttgct
    13501 gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc atctacaacc tgtcccgtcg
    13561 ttttgcccaa cgcggcttct cccctcgtga attccgcctg acgatgactc gtggcgatat
    13621 cggtaactat ctgggcctga cggtagaaac catcagccgt ctgctgggtc gcttccagaa
    13681 aagcggtatg ctggcagtca aaggtaaata catcaccatc gaaaataacg atgcgctggc
    13741 ccagcttgct ggtcatacgc gtaacgttgc ctgatttttc cgcataactc actatccttc
    13801 tgtcatatca ttaaattttt ctgatttatt gatctggcag aaggttcatc actgtttcat
    13861 tcaccagata tgggttaatc ttttaattac aaactgcgtt gacagttgtt gtaaggagac
    13921 cctgtatggc tatgtatcag aacatgctcg ttgttatcga tcctaaccag gacgaccaac
    13981 cagcattgcg gcgagctgtt tatttacatc aacggattgg tggcaaaatt aaagcctttt
    14041 tgccgatcta tgacttctca tacgaaatga ccaccctgct ctccccggac gaacgtaccg
    14101 ctatgcgtca gggcgtcatc agccagcgta cagcctggat ccacgagcag gcaaaatatt
    14161 atctcaatgc tggcgttccc attgaaatta aagtggtctg gcataaccgt cccttcgaag
    14221 ccatcattca ggaagtgatc agcggcgggc acgatttggt gctaaaaatg gcgcaccaac
    14281 atgaccgtct ggaagcggtg atttttacgc caacggactg gcatctgtta cgcaaatgcc
    14341 caagcccggt gtggatggtg aaagaccagc cgtggccgga aggaggcaag gcgctggtgg
    14401 cggtgaatct cgccagtgaa gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag
    14461 agacgatcga actggcagaa caagtcaacc ataccgaagt tcatctggtt ggcgcttatc
    14521 cggtaacgcc aattaatatc gcgattgaac tgccggaatt tgatccgagc gtttataacg
    14581 atgccattcg tgggcaacat ttgctggcaa tgaaagctct gcggcagaaa ttcggcatta
    14641 atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg
    14701 agcacttaca ggcggggatt gtggttctgg gcacggtagg acgcaccggt atttcagcag
    14761 cattcctcgg caacacggcg gaacaggtga ttgatcatct tcgctgcgat ctgttggtca
    14821 ttaaacctga ccagtatcag acacccgttg aactggatga cgaagaagac gattaacgac
    14881 tcttcaataa ctgacaaggc ccgagttatc gggcctattt tttacttact gctcttcgcc
    14941 gccaaggaaa taaattccca acgggattgc cagcaacaca gtaaagacca ggctataaac
    15001 aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc cactcagaca gcggaatatt
    15061 gtactgttct ataacgccgc cgatagtcgc gcgagtcaca accgacgccg caataatgaa
    15121 aaccgccaac aaaccaaaga gaaatttctt ccctttcgct gacttcagtt tcgccataat
    15181 catgatgtaa tccttgtgca gacaaatttc ttgttaccta aacgtaacaa taccatgaca
    15241 tttcaccgtt gtctgctctg aaaacctcac ctcttagctg gatttacaat caacgcattg
    15301 attagttagc taatgttgct acttccttcc ctgactattg atgatctgaa tgaattgtcg
    15361 cggtaaaata gccccgccag gggtttccgg catgacgaca attatctgga ggagcctgat
    15421 aaaacaatga tcgctgaact gtttacaaat aatgcgctta atctggtcat tattttcggt
    15481 agctgcgcag cattgattct gatgagcttt tggtttcgcc gcggaaatcg taaaagaaaa
    15541 ggttttttat tccatgcggt gcaattttta atctacacca taattatcag tgctgttggt
    15601 agcattatta attatgttat agaaaactat aaactcaaat ttatcacccc aggcgttatc
    15661 gattttatct gtacgtccct gattgcggtt attctgacga ttaagctatt tttgctgatt
    15721 aatcagtttg aaaaacagca gattaaaaaa ggtcgcgata tcaccagtgc gcggattatg
    15781 tcgcgcatta tcaaaatcac cattattgtg gtgcttgttc tgctttatgg cgaacatttc
    15841 ggcatgagcc tttctggctt gctgaccttt ggtggtattg gtggtctggc tgtcggtatg
    15901 gccggtaaag atattctgag taatttcttt tccgggatca tgctctattt cgaccgtcct
    15961 ttcagtattg gcgactggat ccgttcaccg gacagaaata tcgaaggtac agtagcggaa
    16021 attggctggc gaattaccaa aattacgacc tttgataatc gtccattgta cgtaccgaac
    16081 tcgctgtttt cgtcgatcag cgtagaaaac ccaggacgaa tgaccaaccg ccgcattacc
    16141 acgaccattg gtttacgtta tgaggatgcg gcaaaagtgg gcgttattgt cgaagccgta
    16201 cgtgagatgc tgaaaaatca cccggccatc gaccagcgac aaaccttact ggtttatttc
    16261 aaccagtttg ccgactcgtc attgaatatt atggtttatt gctttaccaa aaccacggta
    16321 tgggctgaat ggcttgctgc acagcaagac gtttatttga agattatcga tattgtgcag
    16381 tcacacggcg cggactttgc cttccccagc cagacgctgt atatggataa cattacaccg
    16441 ccggaacagg ggcgctaaat cagacatcac tggcgttatc cacaagggta acgccagttc
    16501 ccatcacatc aatgcttcac aatatacata gtccggctgt acgcaacatc ttccgggtta
    16561 ttaatgggat aacctttcag ccacggcttg attaatcgtc cattggtata ttgataaatt
    16621 ggtgcaatcg gtgcttgctc catgaggatt ttttctgccg cgttgtaatc ggcattacgc
    16681 gctttaacgg tattttccat cgatgcctgg gccagaactt tgtcgtatgc cggattgtta
    16741 aagcgtgaaa tatttcctga atgcgttgac gttaataatg tcaggaaagt ggagggttca
    16801 ttataatccc ccacccatga ggcgcggata acatcaaaat tgccggtgtt acggctatcg
    16861 atataggttt tccattcctg attttgcaat ttaacatcta cgccaaggtt ctttttccac
    16921 atcgatgcta cagcaatcgc aattttttga tggttttctg aagtgttata caaaagcgtc
    16981 agcttcagcg gtttttgcgg accataacca gctgcgctca acaaagtttt tgcctgcgca
    17041 ttcagttctt cctgactcat ttgttcaaac ggcgaaggtt ccggcgtaaa tccggcggta
    17101 acatctggtg taaaatgcca cgctggcttt tcgcccgttc ccaacacttt ttcggtcatc
    17161 aggcggcgat ctatcgtcat acttaatgcc aggcgaacgc gctgatctgc cgtcggccct
    17221 ttttgcgtgt taaacgcata ataataggtc ccgagctgtg gcggcgtata aacctgcccc
    17281 ggaatatcct tcaacagctt ctgatacata tttttcggga aggattcggt gatatcaata
    17341 tcccccgcga ggtaacgctt agtggctgcg gattcctgat taattggcag gaaggtcact
    17401 ttttgcagta ccgttttggc gttatcccaa taatgggtat tcggtaccac gaccagtttt
    17461 tcattgacta cgcgatcttt aagaacataa gcgccattgc cgatcagatt tccgggtttc
    17521 gtccactctt taccgctttc tacgttggct ttttgcaccg ggaagaaggc aaagttagcg
    17581 gttaaattca caaaccacgg caacggttta tcaagctgaa ttttcaaagt atgggcatca
    17641 actgcggtga cgccaagctg gtctggcgta gctttaccat caataatcgc ctgtgcgttg
    17701 ttgattcccg ccagcgcggc aaaccatgca aatggcgata gcgttttcgg gtccaccaga
    17761 cgttgccagc tgtagacaaa atcttgcgcc gttaccggtg tgccatccgc ccattttgcg
    17821 ttatcgcgca gggtaaaagt ccagatacgg ttgtcattac ttttccactg agtcgcaacg
    17881 ccggggacaa tctccccttt ctcgttctga ttcaccagtc cttcaaacag atcgcgaatg
    17941 acctgaatct ctggcaggcc cacggcttta gcgggatcca gcgacgcagg ctcatcttta
    18001 atgtggcgca ccagctcctg cttctctgcc agtactgtgc cgctcggaac ttctgcagca
    18061 tacgaaagag aaatgctgct gaccaacagc gcacaacacg tgactgaaac agagtgcttc
    18121 ataagtttcc ctctaaccga cattaaaatt gatgcgtaat tatttgtttc gccttaaaga
    18181 aatgcaaaca acttccatca aaaaagtgac acaggaagca taaaatgttt tatgtatcaa
    18241 tatattaaat cagatttagt gtaaataatc gacgccaaaa tgcggtaaag cacatagaac
    18301 accgaacact acgttcaggc ttcgcacgtt caaacc
//
LOCUS       DAZFDP010000095        18160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFDP010000095 DAZFDP010000000
VERSION     DAZFDP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            202..2391
                     /locus_tag="ACHJ1G_003279"
     CDS             202..2391
                     /locus_tag="ACHJ1G_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4765076.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2441..3643)
                     /gene="nupC"
                     /locus_tag="ACHJ1G_003280"
     CDS             complement(2441..3643)
                     /gene="nupC"
                     /locus_tag="ACHJ1G_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4765077.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            3979..5217
                     /locus_tag="ACHJ1G_003281"
     CDS             3979..5217
                     /locus_tag="ACHJ1G_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4765078.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(5358..5684)
                     /gene="ypeC"
                     /locus_tag="ACHJ1G_003282"
     CDS             complement(5358..5684)
                     /gene="ypeC"
                     /locus_tag="ACHJ1G_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4765079.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(5799..7055)
                     /locus_tag="ACHJ1G_003283"
     CDS             complement(5799..7055)
                     /locus_tag="ACHJ1G_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4765080.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            7259..8224
                     /gene="glk"
                     /locus_tag="ACHJ1G_003284"
     CDS             7259..8224
                     /gene="glk"
                     /locus_tag="ACHJ1G_003284"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4765081.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            8443..8769
                     /locus_tag="ACHJ1G_003285"
     CDS             8443..8769
                     /locus_tag="ACHJ1G_003285"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4765082.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            8791..10038
                     /locus_tag="ACHJ1G_003286"
     CDS             8791..10038
                     /locus_tag="ACHJ1G_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4765083.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            10053..11138
                     /gene="ypdF"
                     /locus_tag="ACHJ1G_003287"
     CDS             10053..11138
                     /gene="ypdF"
                     /locus_tag="ACHJ1G_003287"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4765084.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            11138..12175
                     /gene="ypdE"
                     /locus_tag="ACHJ1G_003288"
     CDS             11138..12175
                     /gene="ypdE"
                     /locus_tag="ACHJ1G_003288"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4765085.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            12200..14695
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_003289"
     CDS             12200..14695
                     /gene="ptsP"
                     /locus_tag="ACHJ1G_003289"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4765086.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(14698..15555)
                     /locus_tag="ACHJ1G_003290"
     CDS             complement(14698..15555)
                     /locus_tag="ACHJ1G_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4765087.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(15568..16302)
                     /gene="ypdB"
                     /locus_tag="ACHJ1G_003291"
     CDS             complement(15568..16302)
                     /gene="ypdB"
                     /locus_tag="ACHJ1G_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4765088.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(16317..18014)
                     /gene="ypdA"
                     /locus_tag="ACHJ1G_003292"
     CDS             complement(16317..18014)
                     /gene="ypdA"
                     /locus_tag="ACHJ1G_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4765089.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         4302 a         4538 c         4879 g         4441 t
ORIGIN      
        1 caaccctcgc tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt tgtaatacag
       61 tctacgtccg ggttagcgcc gcctggtgaa agcatcattg gatgaaaaat cggcaacagg
      121 ctggcccctg tttgcttcgc gatgcgaata aacttattat ttgtgtgcct gaaaaccccg
      181 atcagtgaga gtagtgtact catgtttgtg gagcataacc tgataaaaaa tatcaagata
      241 ttcacactag cgtttacgct caccgtggta cttattcagc tatcccgttt tatttcgcca
      301 cttgccatta tccattccag ttatatcttt ctggcgtgga tgccactgtg cgtaatgctg
      361 tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg ttttatgcgg gatgttttgc
      421 accaatctgt ggaactttca tctctctttt ttacagactg cggtcatgct cggtagccag
      481 acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc agctggggac gcgttggcgt
      541 tatggattga ccagccgata tgtctggcaa cgtctgttct ggcttggttt ggtgacgccg
      601 atcggcatca aatgcagcat gtatcttgtg gggagcttct ttgatttccc gctaaagata
      661 tccacctttt tcggcgatgc ggatgccatt ttcacggtcg ttgatttgct aagccttttc
      721 accgctgtgc tgatttacaa catgcttttc tactatctca ctcgcatgat tgtaagtccc
      781 cactttgcgc agatattgtg gcgcagggat atcgctccgt cgttgggcaa agagaaacgc
      841 gcatttacct taagctggct ggcagcgtta agcgtgctgc tacttctgtt gtgcacacct
      901 tatgaaaacg actttattgc cggttacttg gtacccgttt tcttcatcat ctttaccctc
      961 ggggtcggta agcttcgcta tccgttttta aatctcacct gggctgtttc aacgctttgc
     1021 ctgctgaatt acaaccagaa ctttttgcaa ggggtggaaa ccgaatattc gctggcattt
     1081 attcttgcgg tgctgatttc ctttagcgtt tgcctgctct atatggtgcg catttatcat
     1141 cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc tgacagatcc gttaacgctc
     1201 ctacccaact ttcgtgcgtt ggaacaagcg ccggagcaag aggcgggcaa gagtttttgc
     1261 tgcctgcgca ttgataatct tgagtttatg agtcgtcatt acggcttaat gatgcgcgtt
     1321 cactgtatcc gctcaatttg ccgtacgctg ctgccgttga tgcaggaaaa cgaaaagttg
     1381 tatcaattgc cgggtagtga actgctgtta gtgctgagcg ggccggaaac ggaagggcga
     1441 ctccagcata tggttaacat cctgaatagt cggcaaattc actggaacaa taccgggctg
     1501 gatatgggct atggtgctgc ctgggggcgt tttgatggaa atcaggaaac cctgcaaccc
     1561 ttgttggggc agttaagctg gctggcggag caatcctgcg cacatcatca tgtgctggcg
     1621 ctggatagca gagaggagat ggtttccggg cagaccacta aacaggtgct attgctgaat
     1681 accattcgca cggcgttaga tcagggtgat ttgctgctct acgcccagcc aattcgcaac
     1741 aaagagggtg acggttatga tgagatcctc gcgcgactga aatatgacgg cggcattatg
     1801 accccggata agtttctgcc ccttattgct cagtttaacc ttagcgcgcg ttttgatttg
     1861 caagtgctgg aatccttgtt gaagtggctg gcaacacacc cttgcgacaa aaaaggaccg
     1921 cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa agaatattgc cgggcggatt
     1981 attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg tcattcttga gatcaccgag
     2041 gagcaggcgt tttctaacgc agaaagcagc atgtacaaca tcgagcagct gcataagttt
     2101 ggtttccgga ttgcgattga tgactttggc accggatatg ccaactacga acggttaaag
     2161 cgtttgcagg ctgatatcat caaaattgat ggcgtctttg tgaaagatat tgtcacgaat
     2221 acgctggatg cgatgattgt gagatcaatt accgatctgg cgaaagcgaa gtcattgagt
     2281 gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc tattgcataa gctcggggtg
     2341 caatatctgc aagggtattt gattggtcgc ccgcagccat tagctgatta acaggcgtaa
     2401 aaaaaccggg gaattatccc cggctttttt atgtatggtc ttacagcacc agtgctgcga
     2461 ttgacgcaga cagcacactc accagggtag agccgtaaac cagcttcaga ccgaagcgag
     2521 aaaccacgtt accttgctct tcattcaggc ctttaactgc acctgcgata atcccgattg
     2581 aagagaagtt agcgaaggaa accaggaaca cagagatgat gccttcagca cgcggagaga
     2641 gcgtggaagc aattttctgc agatccatca tcgcaacgaa ctcgttggaa accagtttgg
     2701 tcgccatgat actgcccact tgcagtgctt cactggaagg aacacccatc acccatgcaa
     2761 tcggatagaa gatgtagccc aggatgccct ggaaggagat gctgtagcca aaccagccag
     2821 taacggtggc aaacagtgcg ttcagcgcgg cgatcagggc gataaagccg atcagcatcg
     2881 cggcaacgat aatggcaact ttgaaacctg ccagaatgta ttcacccagc atttcgaaga
     2941 agctctgacc ttcgtgcagg ttggacatct ggatgttttc ttcactggca tcaacacggt
     3001 aaggattgat cagcgacagc acgataaagg tgctgaacat gttcagtacc agcgcagcaa
     3061 cgacgtattt cggttccagc atggtcatgt atgcaccaac gatggacatc gacacggtgg
     3121 acattgccgt ggcagccatg gtgtacatac gattacggga gattttgccg aggatatctt
     3181 tataggcaat aaagttttca gactgaccca gaatcaggga gctgacggcg ttaaaggatt
     3241 ccagtttgcc catgccgttg actttggaga gcaggaaacc aattgcgcgg atgatcaccg
     3301 gcaacacgcg aatgtgctgg agaataccga tcagtgcaga gataaagacg attgggcaca
     3361 gcactttcag gaagaagaat gccaggcctt gatcattcat gctaccaaag acgaagttag
     3421 tcccttcgtt ggcaaatccg agcagttttt cgaacatttc ggagaagcct ttcacgaagc
     3481 ctaaaccaac gtcggagttc aggaagaacc acgccagtaa cacttcgata acaagcagtt
     3541 gaataacata acggatacga atttttttgc ggtcgctgct taccagcagt gcgagaatcg
     3601 caacaacggc aagtgccagt acaaaatgaa ggacgcggtc catatttgct ccaaatatga
     3661 ggcaggttaa atttccgtgc acattctatg taacatggtt aaggaaaacg agatctgaca
     3721 cacactataa tgacgtcctg tgacaagctt caaaaatagt gatctgtcat acatttctgc
     3781 tatgttgtgt atggaagctg aaagttatgt aaatgtgcta acattaaaca taaccataac
     3841 attcaagtca tcaatctttt cgtagtcata atcctggtct atcaaagaaa tcaccacaac
     3901 ccattcaaat gaatttgata atcattctcg tttggcatag catgaaacat agcaaaggct
     3961 atgttttaga ggcacaagat gacgaactat cgcgttgaga gtagcagcgg acgggcggcg
     4021 cgcaagatga ggctcgcatt aatgggacct gcgttcattg cggcgattgg ttatatcgat
     4081 cccggtaact ttgcgaccaa tattcaggcg ggtgctagct tcggctatca gctactgtgg
     4141 gttgtcgttt gggccaacct gatggcgatg ctgattcaga tcctctctgc caaactaggg
     4201 attgccaccg gtaaaaatct ggcggagcag attcgtgatc actatccgcg tcccgtagtg
     4261 tggttctatt gggttcaggc agaaattatt gcgatggcaa ccgacctggc ggaatttatt
     4321 ggtgcggcga tcggttttaa actcattctt ggtgtttcgt tgttgcaggg cgcggtgctg
     4381 acggggatcg cgactttcct gattttaatg ctgcaacgtc gcgggcaaaa accgctggag
     4441 aaagtgattg gcgggttact gttgtttgtt gccgcggctt acattgtcga gttgattttc
     4501 tcccagccta acctggcgca gctgggtaaa ggaatggtga tcccgagttt acctacttcg
     4561 gaggcggtct tcctggcagc aggcgtgtta ggggcgacga ttatgccgca tgtgatttat
     4621 ttgcactctt cgctcactca gcatttacat ggcggttcgc gtcaacaacg ttattccgcc
     4681 accaaatggg atgtggctat cgccatgacg attgccggtt ttgtcaatct ggcgatgatg
     4741 gctacagctg cggcggcgtt ccacttttct ggtcatactg gtgttgccga tcttgatgag
     4801 gcttatctga cgctgcaacc gttgttaagc catgctgcgg caacggtctt tggattaagc
     4861 ctggttgctg ccggactgtc ctcaacggtg gtggggacac tggcagggca ggtggtgatg
     4921 caggggttca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac catgctgccg
     4981 tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat gagtcaggtg
     5041 ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac cagtgacagc
     5101 aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg ggtgattgta
     5161 gtgctggtcg tggcgctaaa tatctggttg ttggtgggga cggcgctggg attgtagttg
     5221 aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat ccgccctacg
     5281 cgttaaagag ccggtttgta ggcctgataa gacgcgctag cgtcgcatca ggcattgtgc
     5341 tccattggag gacgcgttta atggcggtgc ccatgtccgc ggccttttcc gcgatgatcg
     5401 tcacgatcgc gccagccttc acgatagcca cgctcatatg ctttacgctt atcccagcca
     5461 cgatgatagc cattatcatg acgccaccag cggtttttgc gccattcata attacgatgc
     5521 cagtaatcac gatctcgcca gtgaccaccg tcccagtaat taccgtaatg atcgcgatcg
     5581 ccaatttgta atttgatcga tggcagtagg gtgatttcac ctgcatttgc tgcaagcggg
     5641 gtaaatgcca tcagggcggc cgccagaaac agtgacctga acattgttat tctccttcac
     5701 gatcgaagcc gtcagcggcc tgttaacgca atattacggg gaggtaaagc cccgtatcat
     5761 cgccataact cttaaatcat aaagaagagc attttttgct aaacattatc gtggggtggc
     5821 tgttgcttcg gacgattgac catcatcatc ggcttacctg ctaataacag ccatgccgga
     5881 agcatgacga tacagagcag cggtagcaat gtggtattgg gtacaacgac tgccgccata
     5941 aaaagactta accagccatc gcgtgttacc accagcacga tgccgagaat agcgcaggaa
     6001 acggttattg ccgctggaac ggcgggaacg tgctcatgca gcatcaaccc taatgccacg
     6061 ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc cactggcggc agcaacgacc
     6121 agggcggcaa gtttaattac cgccagcaaa aagtaatcgc tggtgctgaa agcctgattt
     6181 gccaccatct gctgcatctc atccagccct ttaaacagcg aaaccggccc accaataact
     6241 cccagaatac cgagaataaa tccgccaata cctagcacga gcaccggatt tttcatttga
     6301 tgcatcatcg cgtgcaaccg tggtaagcac catacggcga ccattcctgc tgcgatggca
     6361 atcgccgcga caattgcacc gctgagaata tcggtcattt ccatctgtcc gtaatgcgca
     6421 atgggcagtg aaaaatgggg atggaaaaat aatccggtag taagtgcacc agctgctgcc
     6481 gccattaatg gcgcaaaaag acgatcccat agcggaactt cactactgcc atttaaggtt
     6541 tgcgaaaata tcaacgccgc cgcaacaggt gtaccaaaca gcgcgccgat ggttccggca
     6601 gaggctaaaa tagtccactc cattcggttg acgcgcggta acagacgagc gccaatggca
     6661 accgcaaggg cgatattgac ggtcatgatc ggatgttccg gccccaggct gacgccgcca
     6721 gcaagaccga gaattaatgc tacgataagt cctggtagcg cagagggcgg aactggtgcg
     6781 ccgatcagcg gttcacaggc ggggtctggt ccggcatgac cctggctgaa acggataacc
     6841 aaccccaccg caatacccgt tagcgttaat acaccgatga tccagagagg tgaatcctgg
     6901 gctatcccca gagttcccgg cagtcgttgc cagagcaaat tctgtaatac cgaggcgatt
     6961 ttcatcacca caatcagaat aagactggac gcaatcccaa ttgccacggc ggggagcgat
     7021 aataacaaca tggttctggc tcgcggatgg agcatgttct attccttatg cggggtcaga
     7081 tacttagttt gcccagcttg caaaaaggca tcgctgcaat tggtgctgaa acgataaagt
     7141 aaatgtgtga cccagatcga tatttacagg gagcctgcct ttccggcgtt gttgttatgc
     7201 ccccaggtat ttacagtgtg agaaagaatt attttgactt tagcggagca gttgaagaat
     7261 gacaaagtat gcattagtcg gtgatgtggg cggcaccaac gcacgtcttg ctctgtgtga
     7321 tattgccagt ggtgaaatct cgcaggctaa gacctattca gggcttgatt accccagcct
     7381 cgaagcggtc attcgcgttt atcttgaaga acataaggtc gaggtgaaag acggctgtat
     7441 tgccatcgct tgcccaatta ccggtgactg ggtggcaatg accaaccata cctgggcgtt
     7501 ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat ctggaaatta ttaacgattt
     7561 taccgctgta tcgatggcga tcccgatgct gaaaaaagag catctgattc agtttggtgg
     7621 cgcagaaccg gtcgaaggta aacctattgc ggtttacggt gccggaacag ggcttggggt
     7681 tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg ccgggcgaag gcggtcacgt
     7741 tgattttgcg ccgaatagtg aagaagaggc cattatcctc gaaatactgc gtgcggaaat
     7801 tggccatgtt tcggcggaac gcgtgctttc tggccctggg ctggtgaatt tgtatcgcgc
     7861 aattgtgaaa gctgacaacc gcctgccaga aaatctcaag ccaaaagata ttaccgaacg
     7921 tgcgctggct gacagctgca ccgattgccg ccgcgcgttg tcgctgtttt gcgtcattat
     7981 gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca tttggcggcg tgtttattgc
     8041 gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc tctggtttcc gtgccgcatt
     8101 tgaagataaa gggcgcttta aagaatatgt ctatgatatt ccggtgtatc tcatcgtcca
     8161 tgacaatccg ggtcttctcg gttccggtgc acatttacgc cagaccttag gtcacattct
     8221 gtaaatcctt cctgttatat cgggaggtaa ctctcccgat aatcttttaa atcatacagt
     8281 taattcaatt tttctttgtg tcccctcaca agaccgacct gcgtcacact tccgtacagc
     8341 gggattaatt ctccagtaaa tgcattattt gtctggtaac ggcgatttgt tttgcacgtt
     8401 cataatttca ctcgtcaact atcactgaat gaggaattaa cgatgagtaa gaaactaatt
     8461 gccttatgtg cctgcccaat gggcctggct cacactttta tggccgctca ggcgctggaa
     8521 gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc ggacggtatc
     8581 cagaatcgcc tgacggcgca ggatatcgcc gaagcaacca tcatcatcca ctccgtggca
     8641 gttaccccgg aagataatga gcgtttcgaa tcacgcgacg tttatgaaat cactttgcag
     8701 gacgcaatta aaaacgctgc gggcatcatc aaagaaatcg aagagatgat tgcctctgaa
     8761 caacagtaat attcgacagg gataattgtt atggccatta aaaaacgcag tgcaaccgtt
     8821 gtgcctggcg catccggtgc ggcagcggca gttaagaatc cgcaggcctc taaaagcagc
     8881 ttctggggtg aactcccgca gcatgtgatg tcagggattt cacgcatggt gccgacctta
     8941 attatgggcg gtgtgatcct cgctttcagc cagctgattg cttatagctg gcttaaaatt
     9001 cctgctgaga tcggcatcat ggatgctctt aatagcggga aattctccgg tttcgacctc
     9061 tccttactga aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt tggttttgcc
     9121 attccgatgt ttgctgcttt tgtggcgaac tctatcggtg gcaaactggc gttcccggct
     9181 ggttttatcg gcggcttgat gtctacccag ccgacgcaac tgctgaactt cgatcccagc
     9241 actatgcaat gggcgacctc ttcgccggtg ccgtccacct tcattggcgc gctgattatt
     9301 tctatcgttg ccggttacct tgtgaaatgg atgaaccaga aaatccagtt accggatttc
     9361 ctgttagcgt tcaaaaccac atttttgcta ccgattcttt ccgccatttt tgtcatgctg
     9421 gcgatgtact acgtcatcac cccgtttggc ggctggatca acggcggtat ccgtactgtg
     9481 ctgactgccg caggtgagaa gggcgcgctg atgtacgcaa tggggatcgc cgcggcgaca
     9541 gcaatcgacc ttggtggccc gatcaacaaa gccgcaggtt tcgttgcctt cagctttacc
     9601 actgaccacg tattaccggt caccgctcgt tctatcgcta tcgttattcc tccgattggt
     9661 ctgggtctgg cgaccattat tgaccgtcgt ttaaccggca aaggcctgtt caacgctcaa
     9721 ctttatccgc agggtaagac cgcgatgttc cttgccttta tggggattag tgagggtgcg
     9781 attccgtttg cgctggaaag ccccatcacc gccattccgt cgtatatggt cggcgcgatt
     9841 gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga atccgctatc
     9901 tgggcatggc cgctggtcac taacctgggc gtctatatgg cggggatcgc gctgggggca
     9961 gtcataactg cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa aggcaaattg
    10021 ttaatcgata gcctgtaaga aggaaaatcc agatgacatt actcgcttcg ctgcgcgact
    10081 ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc acggcagaac aaacagccgc
    10141 atctggggat ctccaccgga tcaggctatg tggttattag ccgtgaaagt gcgcacattc
    10201 tggtagattc gcgctatttc gttgaggtgg aagcccgtgc gcaaggctac cagctgcatt
    10261 tgcttgacgc gacgaacacg cttaccacta tcgtcaatca aatcattgcc gatgagcagt
    10321 tgcaaacgct cggttttgaa ggccagcagg tgagttggga aaccgcgcac cgctggaagt
    10381 ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa atcaaaacgc
    10441 cagaggaggt ggagataatc cgcctcgcct gtgggattgc cgatcgcgga gcggagcata
    10501 ttcgccgctt tattcaggcg gggatgagcg agcgcgagat agccgctgaa ctggagtggt
    10561 ttatgcgcca gcagggcgca gaaaaagcct cttttgacac cattgtcgcc agcggctggc
    10621 gcggggcgct gccgcacggc aaagccagcg acaagattgt tgcagcgggc gagtttgtca
    10681 ctctcgattt tggtgcgctg tatcagggct actgctctga tatgacgcgc accttgctgg
    10741 tgaatggcga aggggtgagc gccgaatctc acccgctgtt taacgtctac cagattgtcc
    10801 tgcaggcaca gctcgcagca atctctgcaa ttcgccccgg cgtgcgctgc cagcaggttg
    10861 acgatgccgc gcgccaagtg attaccgagg caggttttag ccactatttc ggtcataaca
    10921 ccggtcatgc tatcggcatt gaagttcatg aagatccgcg tttttcaccg cgggacacca
    10981 cgacgctaca gccaggcatg ttactgaccg tggagccggg gatttatttg ccagggcaag
    11041 ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca aggcgcagaa gtgctctacg
    11101 ccatgccgaa aacagtgttg ctcacgggag aggcataatg gatttatcgc tattaaaagc
    11161 gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga tcctgctgga
    11221 agaagcggat cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg tgctgatccg
    11281 cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc catatggacg aagtgggatt
    11341 tatggtgcgt agcatctccc gcgaaggggc aattgatgtg ctgccggttg gcaacgtgcg
    11401 catggctgcc cgccagctgc agccggtgcg catcaccacc cgtgaagagt gcaaaattcc
    11461 aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc gccatgcgcg tggacattgg
    11521 tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc gcgtcacctt
    11581 tgatacaact tttcaggttc tccctcacca gcgggtgatg gggaaagcct ttgatgatcg
    11641 cctcggttgc tatctgctgg tgacgttact acgtgagctg cacgatgccg aactgcctgc
    11701 ggaggtgtgg ctggtggcaa gttccagcga agaggtggga ttacgcggtg ggcaaaccgc
    11761 tacccgcgcg gtgtcgccgg acgtcgccat tgtgctggat accgcctgct gggcgaaaaa
    11821 ctttgattat ggcgcggcta accatcgcca gattggtaac ggcccgatgc tggtgttaag
    11881 cgacaagtca ctgattgcgc cgccaaaact taccgcctgg atcgaaaccg tggcggctga
    11941 aattggcgta ccgttgcagg cagatatgtt cagcaacggc ggcacggacg gcggagcggt
    12001 gcacttaacc ggtactggcg tacccacagt ggtaatgggg cctgccaccc gccacggaca
    12061 ttgcgcggca tcgattgccg attgccgcga cattttgcag atggagcaac ttttatctgc
    12121 ccttattcaa cgtcttacgc gtgagacggt tgttcaactg acggatttca gatgatctcc
    12181 tgattaaccc ggagcggtta tgttaacgat tcaatttctc tgtcctttgc ctaacggtct
    12241 acatgctcgt ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa gcgaaatcac
    12301 ctttttcaac catcgccaga acgcaaaggc cgatgctaaa agctcgctgg cgttgattgg
    12361 aactggcacc ttatttaatg acagttgcag cctgaacatt agcggcagcg atgaagagca
    12421 ggcgcggcgc gtgctggaag agtacatcct ggtgcgcttt atcgacagcg acagcgttca
    12481 gccgacgcag gcagaactga cggcgcatcc gctgccgcgt tcattaagcc gccttaaccc
    12541 ggatttactg tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc tgaccctgtt
    12601 acaaagcgac agcctcgaca gttatcgggc aatccccgcc agtgcgcaag attccacccg
    12661 gctggagcac agcctggcaa cgcttgccga gcaactgaac cagcaattgc gtgagcgtga
    12721 cggcgaaagc aaaactatcc tcagcgccca tttgtcgctg attcaggatg atgaattcgc
    12781 gggcaatatc cgtcgtctga tgacagaaca gcatcaaggg ctgggggcgg cgatcatcag
    12841 caatatggag caggtttgcg ccaaactttc tgcttctgcc agtgattatc tgcgcgaacg
    12901 tgttagcgac attcgcgata ttagcgaaca gttgctgcat atcacctggc cggaactgaa
    12961 gccgcgcaac aatctggtgc tggaaaaacc gactattctg gtagccgagg atttaacccc
    13021 aagccagttt ttgagcctcg atttaaaaaa tcttgcaggc atgattctgg agaaaactgg
    13081 gcgcacctcg cataccctga ttctgacccg cgcctcggcg atcccggtac tgagcggcct
    13141 gccgctggat gcgattgccc gttatgccgg gcaaccggcg gtgcttgacg cccagtgcgg
    13201 cgtgctggcg attaacccga atgacgcggt gagcggttat tatcaggtcg cacagacgct
    13261 ggcggataaa cgccaaaaac aacaggcgca ggccgccgcg cagctggcct attcccgtga
    13321 taacaagcgt attgatattg cggcgaatat cggcaccgct ctggaagcgc caggcgcgtt
    13381 tgccaacggc gcggaaggcg ttggactgtt ccgtactgaa atgctctata tggatcgcga
    13441 cagcgcgccg gacgagcagg agcaatttga agcctaccag caggtgctac tggcagcggg
    13501 cgacaagccg attatcttcc gcacaatgga catcggtggc gataaaagca ttccttatct
    13561 gaatattccc caggaagaga acccgttcct cggctatcgc gcggtacgta tttacccgga
    13621 atttgctggc ctgttccgca ctcaactacg ggcgattttg cgcgccgcca gttttggcaa
    13681 cgcccagttg atgatcccga tggtccacag tctcgatcag atcttatggg tgaagggcga
    13741 gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag agacgattac
    13801 gctggggatc atggtggaag tgccgtcggt gtgctacatc atcgatcact tctgcgatga
    13861 ggtcgatttc ttcagtatcg gctccaacga tatgacccag tatctgtatg cggtcgatcg
    13921 taataacccg cgcgtatcgc cgctgtataa cccgattacg ccatcgttcc tgcgcatgtt
    13981 gcagcagatt gttaccactg cgcatcagcg gggcaaatgg gtaggcattt gcggtgaact
    14041 gggcggtgaa agccgttatc tgccgctact gcttgggctg ggcctggatg agctgagtat
    14101 gagtagcccg cgtattccgg cggtgaaaag ccagcttcgt caactggata gcgaggcgtg
    14161 tcgggaactg gcgcgtcagg catgtgaatg ccgcagtgcg caggaaattg aagcgttact
    14221 caccgccttt acgccggaag aagacgttcg cccactgctg gcgctggaga atatctttgt
    14281 tgatcaggat tttagcaata aagagcaggc gatccagttc ctgtgcggca acctcggcgt
    14341 taacgggcgc actgaacatc cgtttgagct ggaagaagat gtctggcagc gggaagagat
    14401 tgtcaccacc ggcgttggtt ttggcgtagc gatcccgcac accaaatctc agtggatccg
    14461 tcattccagt atcagcattg cccggctggc gaaaccggtt gactggcagt cagaaatggg
    14521 cgaagtcgaa ctggtgatca tgctgacgct gggtgctaac gaagggatga atcatgtgaa
    14581 agtcttctcg cagctggcgc gtaaactggt gaataaaaac ttccgccagt cgttgtttgc
    14641 cgcgcaagat gcacaaagta tcctgacgct gctggaaaca gaattaacct tctgacgtta
    14701 gccctgaaaa cgggcgctgt actctcccgg cgtcagacca aactgacgcc ggaaaacgcg
    14761 acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa ttgacagatg
    14821 atatttctgc aaaatcatcc tcgccttcgc cattcgcacc caacgcacat actcgataaa
    14881 acgcatcgtt ccatgctgag caaacagttt tgacagatga ttgggcgtga tattaaaaaa
    14941 ctgcgccacg ctctcgcggg tgagcggctg ggcataatta tcctgaaccc agttacaaat
    15001 gctgtgatag agaaaatcgc cgcgcggttg cgaattaccc ggcggcatat tcaccacgtt
    15061 gcggcaaaga tgcagcaaac ttaacaccag tggctgaatg atattctgct ccagcggcga
    15121 acggctaaga tgggtgaggg cggtaagcat cgcttcacct tcaccgcgtt gcaggctggg
    15181 aagctggatc tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt aaaacgataa
    15241 cccaagccag gtcggtgcga acaccaggct taacagcatc accggtttgt tgttgatcgg
    15301 taaattagcc gcgcgtgccg gaataaacag catttctcct tccaccattc gctgctgctt
    15361 tgcttccagc aggttgccgt attcgccgcg cagcacgata tccagacggg gaaaatcaat
    15421 acataaactg cccaccggta acgaggcagg ctggctggca aaccagacgc gccccagtag
    15481 ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa aattgttgat cggcgggcaa
    15541 gcctggtgct ttcatgaaag ttcccgatta aagatgcatt aactggcgaa attctttcac
    15601 tttgctgcga ctgaccggca cttcaaaatc cagatctttc agtcgcagaa tgtaggtgtt
    15661 attaaaccac ggttcgattt cgcgtatttt gttcagattg acacaaaatg agcgatggca
    15721 gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca tcggcattac
    15781 gtaggattca cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg cgtaatagat
    15841 atcgttaatt ggcgtgacga ttattcgctc atctttcacc agattaatgg tgtcattctc
    15901 acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg ccgcttccag
    15961 tttttgcagc atcccggtaa tacgtgactc ctgatacggt ttgagaatgt agtcaaacgc
    16021 ctccagttca aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca caataaacgg
    16081 tttattggcg aactggctga tgttttgcgc cagcaacacg ccatccagcg acggaatatt
    16141 gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca cgtccagacc
    16201 gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct ttaattagcc agctcagttc
    16261 ctgttgtgcc aggaattcgt cttcaacaat gatgactttc acaatatcac tccggctcaa
    16321 agcaataacg tagcctgtga ggcgactggg gtgcgttggt taggaatgta aaacgcaatt
    16381 tccgtccccg gctccaggcg gcggatatgc agcccctcgc catacaataa cttcacgcga
    16441 tgatggacat tcagcaggcc aattttattg cccggcattt cattcgcttc gacccgctca
    16501 atcacctttg gatcgatgcc gtgcccggta tctcgcaccg caatgcgtac ccgatttccg
    16561 cactctgcaa cgctgatggt gacaacgcct ttacctttgc aaggctgaat accgtggaca
    16621 atggcgttct ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt cacctcttca
    16681 tcaatatcat agataaccgt cagcttgtca ccaaagcggg cctgctcaat ggcaatatag
    16741 tctttaattt gatacagctc ttttttgata tcgatttgct catcgtcttt taattcaatg
    16801 ttatagcgca gataacgcga cagattaaag atcaactggc gagcggtatc cggattcaga
    16861 cggattgacg atgaaatagc gttcagagcg ttaaacagaa aatggggatt aattttgctt
    16921 tgcagggcgc gaagctctgc cttatttgcc atttcacgta gctgctcggc gcgtgaaacc
    16981 tccagttgcg tggagatgat ttgcgacaga ccgaccgcca tttcctgtaa tgacgaggtg
    17041 atctgatgcg cgtggcagta gtaaattttc agcgttccgg tcacgacccc tttctcccac
    17101 aatgggatca ccagcatgga atgaatctct ggtgtgcggt gggcttcatc attgttttta
    17161 atgatgattt ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa gtcatcgcca
    17221 ttctgatagt tatgttcacc cacgccaaca taggccagca catgatcggt attagtaatc
    17281 gccaccgcat cggcgtgaat gtcatcgcgg ataatttcgc agaccttgcg taatgactcg
    17341 ctattgacat ggcgaaacag cggtagcgtt ttgttggcga tatccagcgc cagcttggcc
    17401 tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt gcaccagaag cacaataaag
    17461 ccgatacaga cgctaccgag gatcattgga atgccgattt tagagacgat atcgatcccc
    17521 aacgcggtag ttggtgccca gacaattacc agaatcatgg tcaacgtctc acacaacatg
    17581 ccgccgagaa taccgacgcg ccagcgctgt gctttgggga ttttcaggtt gatccagccc
    17641 gatatacaac ccgccagaat gctggtgata aagcagggga tcgccgtcac gccgccgata
    17701 tcaattaaat accggtgaat acccgcaatc acgccggtaa tgatgcctac ccacgggcca
    17761 aacagaatcc cgccggacat caccgcgata atacgtacgt tcaccagcga gccttctacg
    17821 ggaacgccgg accaggtgct gaacagggcg aacagcgaga aaatggcggt aacggcgagc
    17881 aattcttttg gggagtgcgc cgacttgtgc aacagttcgc gaaacaggcg gatacggatg
    17941 agaaagaaca ggcagataag cattaacgct gcccgatcga agaccgccag cagcatgttg
    18001 aatatttcgt gcacgggtga ctctgaataa ggattaaacc tctatgatag gtaacctgaa
    18061 ggctgatgac cagcaggccg tttttgagga aaaaccataa atttaaaagg ggattgaaag
    18121 ataaatggtg tattagcaat gtatctcttg ataatgcact
//
LOCUS       DAZFDP010000096        18094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFDP010000096 DAZFDP010000000
VERSION     DAZFDP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(329..655)
                     /locus_tag="ACHJ1G_003293"
     CDS             complement(329..655)
                     /locus_tag="ACHJ1G_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4765090.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQL"
     gene            complement(652..915)
                     /locus_tag="ACHJ1G_003294"
     CDS             complement(652..915)
                     /locus_tag="ACHJ1G_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4765091.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(987..1853)
                     /locus_tag="ACHJ1G_003295"
     CDS             complement(987..1853)
                     /locus_tag="ACHJ1G_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4765092.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEICEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(1938..2135)
                     /locus_tag="ACHJ1G_003296"
     CDS             complement(1938..2135)
                     /locus_tag="ACHJ1G_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4765093.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSTALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2147..2635)
                     /locus_tag="ACHJ1G_003297"
     CDS             complement(2147..2635)
                     /locus_tag="ACHJ1G_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4765094.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(2632..3009)
                     /locus_tag="ACHJ1G_003298"
     CDS             complement(2632..3009)
                     /locus_tag="ACHJ1G_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4765095.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(3099..3467)
                     /locus_tag="ACHJ1G_003299"
     CDS             complement(3099..3467)
                     /locus_tag="ACHJ1G_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4765096.1"
                     /translation="MSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQESNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(3547..3768)
                     /locus_tag="ACHJ1G_003300"
     CDS             complement(3547..3768)
                     /locus_tag="ACHJ1G_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4765097.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(3837..4313)
                     /gene="radC"
                     /locus_tag="ACHJ1G_003301"
     CDS             complement(3837..4313)
                     /gene="radC"
                     /locus_tag="ACHJ1G_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4765098.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4329..4814)
                     /locus_tag="ACHJ1G_003302"
     CDS             complement(4329..4814)
                     /locus_tag="ACHJ1G_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4765099.1"
                     /translation="MTTVLHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(4907..5725)
                     /locus_tag="ACHJ1G_003303"
     CDS             complement(4907..5725)
                     /locus_tag="ACHJ1G_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4765100.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLLLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6052..8898)
                     /locus_tag="ACHJ1G_003304"
     CDS             complement(6052..8898)
                     /locus_tag="ACHJ1G_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO4765101.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKCAGVAVALSLAA
                     VTSVPALAADTVVQAGETMSGGTLVNHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTAGNTAISSGGLQFVGAGGKATDTIINEGGGQSLKGLALNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGYALDTTLNGGNQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTIINQKGKLQVDAGGVAVDVTQHQGGALVTSTAATVLGSNRQGNFAVE
                     NGKAEGVVLESGGRLDVLENHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(9265..10137)
                     /locus_tag="ACHJ1G_003305"
     CDS             complement(9265..10137)
                     /locus_tag="ACHJ1G_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4765102.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRNILPELDLVLWLIKADDRALSVDEYFWRHILHQGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            complement(10221..11138)
                     /locus_tag="ACHJ1G_003306"
     CDS             complement(10221..11138)
                     /locus_tag="ACHJ1G_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765103.1"
                     /translation="MVQKILSDNVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKAQAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQKNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKEKNIALENLASFPGSADIYHA
                     IHLSFDIWVTHPSASPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRARRLSTGEMLHDLIVEEYKRYRHSR"
     gene            complement(11970..12167)
                     /locus_tag="ACHJ1G_003307"
     CDS             complement(11970..12167)
                     /locus_tag="ACHJ1G_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765104.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            12236..12433
                     /locus_tag="ACHJ1G_003308"
     CDS             12236..12433
                     /locus_tag="ACHJ1G_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765105.1"
                     /translation="MAQYDDDEHALQVSMRGREHHHFRILSHLPEATGISCGYRQPLC
                     GMPGARTDPSDPSVLRSASDG"
     gene            complement(12339..12941)
                     /locus_tag="ACHJ1G_003309"
     CDS             complement(12339..12941)
                     /locus_tag="ACHJ1G_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765106.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHNLT
                     PGWVSFNGEKPEIAIVPRSLHRLIYGPDKRATPPLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(13028..13234)
                     /locus_tag="ACHJ1G_003310"
     CDS             complement(13028..13234)
                     /locus_tag="ACHJ1G_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4765107.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            13951..14163
                     /gene="hha"
                     /locus_tag="ACHJ1G_003311"
     CDS             13951..14163
                     /gene="hha"
                     /locus_tag="ACHJ1G_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4765108.1"
                     /translation="MEVKTKEDWLYQLRRCSSRETLEKVISHTRYKLTPAELKAFNSA
                     VDHWLAELTMNKLYDRVPASVRKYVT"
     gene            14632..15201
                     /locus_tag="ACHJ1G_003312"
     CDS             14632..15201
                     /locus_tag="ACHJ1G_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4765109.1"
                     /translation="MTYEYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRCEGKRVHTTRLHYVWAREFGENKGKKHYHLILLVN
                     RDTWCGAGDYRDPGSLAGMIKQAWCSALGVDAGCYATLANFPSRPVVWLERDDDAGLR
                     DVLNRADYLAKEYTKVNGTGERNFGCSRN"
     gene            15462..15845
                     /locus_tag="ACHJ1G_003313"
     CDS             15462..15845
                     /locus_tag="ACHJ1G_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4765110.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSSLRYHPQY
                     DTERPWFEHTDEGLTEHGHECPYVRPERREILLIKRLQQWVPDALPVVRKAHRHCRQC
                     HHDYYGERYCTHCHTGRFSEEAVAG"
     gene            15842..16255
                     /locus_tag="ACHJ1G_003314"
     CDS             15842..16255
                     /locus_tag="ACHJ1G_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4765111.1"
                     /translation="MNGLKTGLGITPGEHIISAASALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALTAQQKAACMVLNPEKSHPYIEDMAMFLSPLPTVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRATCTKYVYRYQPDLLRDPE"
     gene            16261..16443
                     /locus_tag="ACHJ1G_003315"
     CDS             16261..16443
                     /locus_tag="ACHJ1G_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765112.1"
                     /translation="MRHNYLIMPVMPLPDPDVVRRFVPRTVVHWLCLLMILFMRHSDR
                     GDIDKHHHLRANSYTE"
     gene            16564..17187
                     /locus_tag="ACHJ1G_003316"
     CDS             16564..17187
                     /locus_tag="ACHJ1G_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4765113.1"
                     /translation="MRTQIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            17283..17516
                     /locus_tag="ACHJ1G_003317"
     CDS             17283..17516
                     /locus_tag="ACHJ1G_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765114.1"
                     /translation="MARSDYDIINLSLKHELNEWLTERGYAGLADNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(17569..17760)
                     /locus_tag="ACHJ1G_003318"
     CDS             complement(17569..17760)
                     /locus_tag="ACHJ1G_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4765115.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFPGKFKPDPREETE"
     gene            complement(17841..>18094)
                     /locus_tag="ACHJ1G_003319"
     CDS             complement(17841..>18094)
                     /locus_tag="ACHJ1G_003319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765116.1"
                     /translation="KQHIAKFITIINGKVQKIIPLNAFNTASYVRKVQFLILVMENHD
                     EQRFSIIEVISGKREGYEGRAQQNAMKEGVREFLVKTED"
BASE COUNT         4272 a         4540 c         4728 g         4554 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatg aaactggtta tataatcaat tgttttatat
      121 cgattgtttt gtctttgttc ttttcggggc caccaatgag gcccccatgt gcaattcgaa
      181 ttcttttgac gcgataatcc ctcttttttg tgctttgttg taatcatttt gtctggttcg
      241 atatgctgaa tcgcggaatc gaagtaacaa caaaatacag agatttataa aatacctgtg
      301 ttagggagcg ttaacgcaca gattttgttt atagctgcca gaaatgatct gatggtttgt
      361 cgtctggctc tgcaagtacc ctgttgatat cccgggaacc atgaacgata cgttcgatac
      421 gaacttcagt atccagtacc ctgaacagca gaaggtaacg accatacgaa caacctctga
      481 tcccagcccc taactcgggt ctttctctgt agagtactgg cgattcggct atcaacagac
      541 attgttggta aagctcttcg gtaaaactga ttgcacggac aggattatcc tgagcgatcc
      601 agtcacctat agcttcaaga tcgtgttctg ctaatggtga aacagtcagt ttcatattgg
      661 ctgttcacct tctgccatct ttttgtattt gtgcgtcagt cgcccgaata cctcttcagc
      721 acttttactt tcaccgctgt tgatgcctgc ggtcacggcg ctctggagtg attcaagctt
      781 tatctgctgt tcccgttctt ccagagcacg aagtccggca cgaatgactt cacttgtatt
      841 gttataccga ccactttcta tttgttcgcg gataaacgtt tcaaagtaag gactgaggga
      901 tacactggta ggcatggtga ttttctcgcc gattgattac tatcaattaa taatagttaa
      961 tattcgcgaa ttgtaaaata cctcttttat gccttgcggc ttttgttcac tgtgcttgcc
     1021 agggcttccg ggtagtgtct ggcaataata tcattaagcc tgtcaaccag ctctggtttc
     1081 ctgaacgtga tgtgcgcgga gcccttctta aagtatctga tgctgaacat ctcatcttca
     1141 tagtcctctt taccctgaac agactggatg tgatcatcca gatgaatggt gatgttttcc
     1201 cggttgtcgg ggatcggttt gccgctgaac aggtgtagca ttctttccag atcggcaagt
     1261 cgatctgcct gtgggccgtt gttcagatga aacccccacc ggtcccacct caccaggttg
     1321 ttgacgataa ttttactgcc aaatttgcag gggcaattgg ttttgtaatt ccagctcagt
     1381 cccctgaaga cgttgatcac ccctcgctca aacacctcat ccttattctg gtgcagttgt
     1441 tcaaatgtgc tcagaatgtt cgcttcgcag atttccggaa aattatcgta ctccagtgac
     1501 cggtaccatg tatcacgggc ttgtgcatcc attaatgaca gcatccctga acgttgcatc
     1561 aggtcacgcc agagttcgcg atcgagattc cgggtgatgg ctttcattgc ggtttccggt
     1621 ttctccatca gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc ccgggcggtt
     1681 ttacctccaa tactttctgt cattgctgat atttcagcca gttgccggat aagtgtgtcg
     1741 atttgcagca gtgtactttt tcgtacagcc agaatgtggc ggatactggt tgagcaaata
     1801 acgtccgtat gcccggtgac cacttccgga cgggaggctg tgatatctga catcatcgtt
     1861 tttccttttg gtttataaac gaaaacgcca gccgtattca ggctgacgtc agtggaatga
     1921 agtctggtga gtaagactca ccggttctgt tgcagaaact gcaactggcg cagggtacgg
     1981 atatcttccc tggcctgttc aatgcagggc agcagtgctg tcgaatccgt tttatcctca
     2041 tcgttgtcaa agataatttc cgattcgcag tcaatattgt cctgcagcca cgcaatcaga
     2101 atatccagcg ctgtttccgt ggttaatgat ttcatgttgt tcctggttac cagttgaaag
     2161 tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac
     2221 gtcgggtaag tgtgacaacc agacggcgga acgtttttga caggcccagg cgcttaagcc
     2281 gcaaaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct gtgaaatgaa
     2341 tccattctga ttcgccggtg tcattcgtct gatgtgacag cgagtacaac caggcattgt
     2401 ccgtttccgt gatatgtgcg gtactgcagc gtatgccggt aagggccgca gatggtggcg
     2461 tgcgctctgc tgatgcccgg tcagattcat caacaacacg aagggagtaa ccgttatcgc
     2521 agaccacgtt aatcagttca gcgagagtaa caccatcgac gtcaacgaca atgcgcccca
     2581 tgttcagtgc ctgcacgtta atgctgtcgg cttccagcgt cagggccagt ttcattgttt
     2641 cgcccccgga tgtttaccca gggtaatgtt atttaccgtt ctgtaattgt cgcgggccat
     2701 caggccggtt gcccggcggg cccggaggat atcaatactg tttattaact gagagcgggt
     2761 acaggtgctg aaccccggct ggtcggttcg taccagtgcg tatttttcca cgagaaagtt
     2821 caccgcatcg cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac gttcatcggc
     2881 aaacggtgtg tcattcagtg tgaggccgta atgctggtcc agcagtcggg acagcagtat
     2941 ctgccagatg gtgacgggag acgggcagcg cgatgcctcc cgtacatgcg tgtcgggtaa
     3001 tgttttcata ctgaagattt tcctgatatg cagagatgag aaatgggaaa gtggcgtggt
     3061 gaaaacacca ggccggagca gaaggttatt ccggagagtt actttttcgt ttcgggcgtc
     3121 ggataaacag ccatataaac gtagccacaa ctgccgaggg tgtcggcttc gcaggtcagc
     3181 ccttttgcat acagtgtcac ggtatgctga tggcggggat tcagttcacc gctggtgagc
     3241 atgagttcca gttgtttcat cagcagcgga aaggcctggt ccaggtggta cgcatccgcg
     3301 tcgctgaacc gccctctgat accggcgcgg tcggcaaggt aatgcaaccg gttactctcc
     3361 tgcaccagac gggcgccaaa acaaggtgtc acggtacagg gcagccccca ccaggggcgg
     3421 tcgtgattgt catcgggatg cgttgtcccg gagaatgtgt ctgacacgat aaagtcctca
     3481 cagaaaatcg gttaagaatg ctccggtggt ggcgataatt cggctcatca gaatgccctt
     3541 cctgactcag ttcagggtga cgctcatcag ccggacatac ggaccaaaac tgtccttacg
     3601 acgttcagca aacacggcca gcacaccggg aatatcctgc acttcacgac cggtatacgc
     3661 ttcagtgctg ccgtgccagc ggtatttgcc ggtacagaac ggaaaaagac gggatgcagg
     3721 atgttgttgg tgaatacgca tggcttcacc acgggtgatg attttcataa tgggatacct
     3781 ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg ttgtgacggt gacgggttaa
     3841 agcaggccgt gttccgcaaa ggagaaaacc tggctgccac cgactatcag atggtccggc
     3901 acccggatat ccaccagagc cagtgcctgt accagacgtt ccgtgataag gcggtctgcc
     3961 ttgctgggtg tgacttcacc agacgggtga ttgtgtgcca gcaccacggc agcggcattg
     4021 tgatacaggg cgcgtttaat cacttcccgg ggatggactt ccgtgcggtt gatggtgccg
     4081 gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac
     4141 tcttcacgct ccagtcccgc catgttcaga atcagccatt ctcgtgccgc acgggtggag
     4201 gtgaaggcca cgccgggttc atgaagatgg cggtccaggg ttttcagggc ccgttgaatg
     4261 aggctgcgct cgccgggcgt catctctcct ggcagaaagg aaatctgttg cattgtgctt
     4321 ctctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
     4381 tcccgcagcc ggtaataatg gaccgtcatg gcataacact ccgtacgaca ggcatgatga
     4441 ctgtacgtca tcagacaggc ggcaataccg gcagcttccg ggctcatttc agcgcggtta
     4501 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cttcatccgg ttcaggtgcc
     4561 ataaatgccc cgccgttgtt cagggtatac agattccaga taccaccgca gtagtcttca
     4621 cacagacggt ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg
     4681 ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggactatggt ggcaaccagt
     4741 tgaggctggt cattccctga tgcagcggtt acggaaacag aaggagtggt ggaattatgc
     4801 aagacggttg tcatgagatt attccttata aaaagtaaat gaatggaaga aaccccgggg
     4861 ggaagggaca gacgtgagtc agaactgcgc tttcagggaa acggcatcag cgcaggctct
     4921 ccagcagcgt ttcagccatc acccacaatg cgcggttgag cttaatgtcg gtgtcgatgc
     4981 tgtgaatggc acgggtatgg atacgttttc ctctggcact gcgaccggaa attccgcctt
     5041 tcagcatatt ttcctggatg gtctgataag cactccacag gtccttaccg taatcctccc
     5101 ggcgtcgtgg cgtcagaatg tcggcagtgg tgacgggctg atgttcgtca ccataacggt
     5161 aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggtaagagc agcgactgca
     5221 tggcatcacg cttctcctct atccggtcaa acacgcccac cacctcgtaa gccccttcaa
     5281 tgactctgtc caccacattt ccccggtgcg gaacacgcac ttcccccaga gactgaccac
     5341 agacgcagcc gttctggcag acaaatctga agtaacccgg cagcatctgg tagctggagg
     5401 taccgtcatg ggagttgagc agaataattt cagggacatg ttgtccgttt atctccccgt
     5461 cacggcgcag gcgcagcatg tgttttgtgt atccccggcg gcccgggtca cgtacgcggg
     5521 tctggcaggc aaagaagggc tgaaaacctt cccgctgcag gctttccagc acggtgatgg
     5581 tggggatcca ggtatagttc tcgcttctgg atgtgtgttt ctcttccccg aatacgctgg
     5641 ggacgacctg catcagttct tcacgagtta aggggcggtc acggcgtatc tggttcgcat
     5701 aaccaaaacg actggctaat cgcatgattt actccttatg aatgagttag cggtataaat
     5761 gaaaaagccg cgtctcccgg agaagacgcg gcctgatggg ggaaatgaat gacgtttatt
     5821 gtctgagaag cccttcactg gcgagctgcg tatttttatg gaaggagagc acccaataac
     5881 cccatcggga tcgggtaata accggccaaa ttgtgaacga ttcagatgcc atttggcagc
     5941 aaaagagcct tcggcaataa acgtgatgta caacccggca tatctgttgc tccctgaaag
     6001 taaaaggccc cggttattat gaccggggcc tgaaggggag tgacctgatt atcagaaagt
     6061 cacattcagc gtggcctgac cgttataacc ttcagcgctg ctgccgctga cactgtgggc
     6121 ataaccggcc tgaacgccca gggtgatatt ttcccggaca cgggcttcca gtccggcctg
     6181 caggtccagt gacgtgccat tccgggacgg tgagaacgtc atgttactgc cggctgcggc
     6241 tgtacccatg ctcatgtctc cccgggagct gaaggtgcgg ataacagaag gctgtaccca
     6301 ccagttcacc ggcagttcac tcacgctgtg ttttgcactg tcgcgcaggg tgtcacggga
     6361 tgaggtgcct ttaccaaagt tcatatcgtt gtggctgccc agacggaaac cggcacgcac
     6421 atgttgtgca ctgccatgcc cgaacttcac ataaccggcg ttgtccttac cgtcatccag
     6481 ggaaagtccc tgccaggtat actgcagttg tggctccagc atcaggttgt cagtgatact
     6541 gaagggcaga ccggtttcca gtgagcccag ccagccccag ccccgggcgc ggaagtcgtt
     6601 attgtccgat gacgctttca tgctgtggcg ggttccctgt gccacaatgt cagcccacag
     6661 gccggaggac gtgtgtgtca gattcaggta tccgcccaga ctgccggcat catcccggac
     6721 cgtgccggcg cgggaaccgt catcatcctt aacatcaacg gaagaatggc ctgcagcacc
     6781 atacacccct gtcgtcagag acataccggc aacctctgtt ctgagcaggt caccctccag
     6841 acggacgaag ccatagctgc cgctgctttc cggcgtggcc ccacgggcaa taccaccgtt
     6901 gttatcgtgc ccgagatgac cgccctgaat gctgagacgg acgctgttat tttcaccgct
     6961 tacaccggtc tgatggctgc gggagcctgc cagaatccgg tcatagtcca ttgcctgtgt
     7021 cagcatggag gcatacaggg ggacttcagc acgataagca ttttcactgc gcaggtacca
     7081 gtcttcatcg ctgtcacggt tcagggtgta gttaaaggcg ccggcctgaa gcgggcgact
     7141 cagggcaaac gcaccttctt ctgtggtggc gccattctgt gcatccacaa cccggatacc
     7201 ctgtccggag gttgccaccc cgaggttact gtttccgaca tttgtaaacg caagccaggt
     7261 tttgccggtt gcctgaccac cattaatcac cagctggtca gaggtattgc tgccatcaag
     7321 gcgaacacgc atattgatgg tgccaccctg accggtgagg ttactggttg tcagtttatg
     7381 gaacgttacc ggggcgttgc cttttgcaac agcacggctc agtacggcgt ccggtgttgt
     7441 ctgattatca aagtaaatct cccctgcgtt aacaatatcg ccggtactga ccacatcacc
     7501 attcagtacc atgctggcgc ctttactgag ctgtgttctg ccactcagac ttcccccggc
     7561 agccagcgtc agtgttccac gatatccgac agtggtgtta ccggcctgtc ccccggcatt
     7621 aaccgtaaag ctgccgccat tttccagtag caggccgctg gcctgaccgg atatgccatc
     7681 aatactgaac ttaccgctgg cattggtccc ctcaacagtg gcaccactgt ctgcaatcag
     7741 ggcaccaccg gatgtcatgg tgacacctgt cgccttacca ccggcagaca ctgccagggt
     7801 accgccgtca tccaccaggg tattctgtgc tgaatggttc tccagtacat ccagacgacc
     7861 accggactcc agaacaacac cttcagcctt accgttttcc accgcgaaat tgccctgacg
     7921 gttgctgcca agaaccgttg ctgcagtact ggtaaccagt gcccctccct gatgctgggt
     7981 aacatcaaca gcaaccccac cggcatcaac ctgcagtttg cctttctggt taataatggt
     8041 attcccggcc acgcccccgt ttttgacaat ctgccagccg tcactgttca caacagtgtc
     8101 agacgctgta ccgccgttgt gcacatactg gttaccgccg ttcagtgtgg tatccagcgc
     8161 gtagccatgt acagtctggt ctccgccggc ataaaccaca gtggtatttg ctgttccttc
     8221 agcagccaca atctgacgac catttttatt gatggtggta cgtacggcat ttccgcgaac
     8281 aaactgcccg gtatcaccat tttctgcatc cggtccccct tccgcgccgg tattcacaac
     8341 ggtgtctgtt gccactgcgc cgggtttgac gacctgccag cccttatcat taatgacggt
     8401 tcctgtggct atcgcaccct catgcatcca ctgctcgcca ccattcagcg tggtgttcag
     8461 tgccagtccc ttcagacttt gtccgcctcc ttcattgatt attgtgtctg tagcctttcc
     8521 cccggcacct acaaactgga gaccgccgct gctgatggcg gtattaccgg ctgtgccgcc
     8581 atcctgtacc cattgcccgc cggtattggc ctcgttatcc ggtccatact ccagcccggt
     8641 actgatggtc attccgttgg tcgtaccgaa gacaatctgg ttgtcatgat ttaccagtgt
     8701 cccgccgctc atggtttctc ccgcctgtac aaccgtgtca gcagctagtg ccgggactga
     8761 cgtgacagca gcaagagaca gagcaaccgc cacaccggcg cattttcccc gtgagcgggc
     8821 cagttcggag gccaccacca gggtgcccgt aatgtgattc cataccagcc tgtagctggt
     8881 gttcagatgt cgtttcatca gcttttcctt acagagggtg aataaaaaag ccggtaccca
     8941 cagatgtggg taccggcagg tacaaaacag catggtcaga taaagtggtt tttcccggac
     9001 ataaatgttt cccctctctc atccgccaga gaagcatcag tccgttgtgt gcaattccgt
     9061 tctgcatgat gaagagattc agctgccttt tcgcaggcca ttaaccacta taaacgatcg
     9121 atagtaacga tcgatacctg ttttatcgat cgttttattc tattaacagc agggaagtca
     9181 cgcaagagaa agaggtgggt atgagggggg catgttttca gttggttaac gcaatgtaat
     9241 caggcatttc ccctggcaga gtgttcagaa gaaaatccag ttccatacag cgcgggcaac
     9301 agagaccacc gagtcacgga cggcacgcag aaccgtgcgt gcaacagagg caatacagac
     9361 gctctccgcc gtgtcaaata tccggtccac cgcaccggta aactgttcac gggcctgaga
     9421 ccggacagat tccgtgcgca gctcgtcctg caggcgggtc atcagggggc tggcggcatg
     9481 gtcgggaagc gctgtcatga gcgcactgac cagcgtatcc agttcccagc cggtgcgggc
     9541 cgatacggca acaaccggat gtacgggccg gaacagccgg aataccgcct ccgttttttc
     9601 gcgaatgttc tgtgcctgtg cgggagaagg ctgaataccg gccatatccc attcatggca
     9661 gggctccgtt ttgtcggcct gcgtcaccac aaacagcacc tgctgatgtc cctggtgcag
     9721 gatgtgtcgc cagaaatact catccacaga cagggcccgg tcatcggctt taatcagcca
     9781 cagtaccagg tccagttcag gcagaatgtt acggtacagg gcttcatact ctgcatccct
     9841 gtcccggctc tcgcccaccc cgggcaggtc agtgataacc atgctgtgac cgtggccact
     9901 cagacggaag cgctgcactt cccgggtgcc ggcgtgaaca tcactgaccg gggtgacctc
     9961 cccctgaaac agtgcattac agagtgagga tttaccggcc ccgcttttac ccataatgcc
    10021 aatcacgggt tcgtgacggg tgagtttgcg cagatgttcc aggatgtgac gggaaagcga
    10081 gtaaggcaga gaggagaggg gtttttcaat tgcctcaatg gcatcagacg gattcataca
    10141 tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga aaggaggcgg gggattcacg
    10201 gagataatat atgtctgaaa ttatctggaa tgtcggtacc ttttatactc ttcaacaatc
    10261 aggtcatgca gcatttcgcc agtactgagt cttctggccc tcgataattc tttgagctgc
    10321 gccctggttt ctgcgtcaag aaaaaactgt acattgactt tatcttcctg catttttttg
    10381 tacttgcgct gtgccttggc cttattgaaa ctgttcctga aattttttat atcgtcaggt
    10441 gaggcagatg gatgggtaac ccatatgtca aatgaaaggt gaattgcgtg gtaaatatca
    10501 gcagaaccag gaaaactggc caggttttcc agtgcaatat ttttttcctt taaataattc
    10561 catgtccata tgcattcatg ttcgttctcc gggaggaggt aactgaaagg gctctttgat
    10621 ttaaatgccc gcatccatct gtctttggtc tggattatcc actcatcccg caaattctta
    10681 tcagaaagaa tatcgagaac tctgagtatt tgtctgaact gtgtcgatga gtccgacggg
    10741 aaggcattcc cttctaaata ctcttcttta agatacagtt tattcttttg aagcaagtct
    10801 gcgcttattt ttgccctgtt tttctggata aactgcatca acagatacca ggaaaataag
    10861 catgcgcgtt cactgcccgc gatccaggat attttttcca gggggaccag gaaggcctgg
    10921 gccttcagac gcataccaga aattatttgt tcaagttcaa aatctgaaag tgtgatgttc
    10981 agattttctc tgtttttctt tatgtaagcg ataaaacgtt ccgggggatc ataaacatta
    11041 aggggcagta cggagatatt acgctctcca agataataag agtaatacgc atttgttctt
    11101 tcattcataa cattatccga caaaattttc tgaaccatcc tctttctctg gctgcttaaa
    11161 aattaaacat aataaggtga taataacatg ataaaaagaa gtttataatg gtatattaag
    11221 atggtcaata aaaaggcaat ctgcgcgctg tatagtcatt ctgacaggaa atgcaggagg
    11281 aagggaaggt aaccggcttc gttatgctga caggaatacc tgttatcatg gaagtgtatt
    11341 catgtggccg acgtagctta agaggatgga agagtggaga ctgcagacag ggagtattga
    11401 aaggtaaaca tgtttcgccc gccagctgca ccggaggcca gcatcacttt ttttacgttg
    11461 taacccggta tgcattccgg ctttgatgat tacagtcaga gggcagcaga ctgacgttat
    11521 ttaaccggac agcaaaatta tggacactga aatatcgtct gttaatccct cgtttgtcgg
    11581 tgtggtattc cctgtattac cggcgtcacc gataaagaga gttataaacg ccagaaaggt
    11641 acctccttta tgccggggaa cacttacgga aaatgaagtc tgaaaaggag cacaatgaaa
    11701 agaggcgtgt cccgtaaata taacgaatca tcgcaggata tacattatca ggtgatacca
    11761 gagggcatta aactgtcgcc acggataaga atatacaaca tttctgataa caaaacatca
    11821 taacgtgttt cagatatgtt ccggcatcag tctgacggat attatttccc ggtaaaacaa
    11881 accggctact gtaacggtta actgtatctg cataaattat ttatgatgaa agaacagtat
    11941 tacggattca aatatcatcc tgtttgcatt caggcggaca ttcccggatg ccggaaagaa
    12001 tatcctgcag gctgagtgag tctcttccac agtgccggga tcgctgtcgc cacgtacata
    12061 ttgtcaccag gatgggtatg taccgcagca tgccatttct ggtgaataac cgcacctgca
    12121 cattcagtct gctgagaacc tggcgtgtat cgggctgtaa agaacatccc tgtccgtggc
    12181 tcctctggtg gtgttgcctg ctcagcaatg acaggatgcc cttaagacgc ccgtcatggc
    12241 tcagtatgat gacgacgaac acgctctgca ggtgagcatg cggggccggg agcatcatca
    12301 tttccgtatt ttgtcacacc ttccggaagc aaccggcatc agttgcggct atcgtcaacc
    12361 gttgtgcggt atgccaggag cacgtactga tccatcagat ccgtcagtgc tgcgctctgc
    12421 ctctgacggt tgatgatcca ttcctccagc tctttttctg atttccgttt caggttctct
    12481 ttccagtctt caaggctgtt tccggtcatt aaatcaagcc agcaaagcca gaccagtttg
    12541 aggcgcagtt ccgtgccacc aaactgacgg aagaggctga tcagtttatt tgccagcgaa
    12601 cgctgcctca ggcgttcaca ttcagacagg agaatgcctt ccagcatcgg atgatgaacc
    12661 agcagatgtt cactggtgca taagttcact accagatcat catccagggg cggcgtggcc
    12721 cgtttgtcag ggccataaat cagccggtgc agactccggg ggacgatggc aatctccggt
    12781 ttttcaccat tgaatgacac ccacccgggc gtcagattat ggaagtagtg gatattcgga
    12841 tgacacacgc cccaggatac ggcgtgccgg gtgaagccgg tcagccattt ctggtagctt
    12901 tcgtggagca gctcgtaacg ggtctgatac tgtggtttca ttttcatatt cctcacgggg
    12961 gataacaggc agcagaatgc cgccggcagg ccggagactg cgtctgctgc agatgaacgg
    13021 ttaagaatta cggacgggat tgtgcaatac gcgcctgcag ccaggcttcc acttcgcttt
    13081 tcagccatcg ggagctgcgg cccattttga tgggcgcagg aaagtcacca tccttgatga
    13141 gtttgtaaaa ccacttatcg gttaagccgg tcagttgagt gataaatgcc atgtcgacca
    13201 tctggtcatc catcagcgaa actggggtga tcatgaaaat gttcctccgg atgttgttaa
    13261 tctcaaccgg cgttggtgat gaaaacaacg caccaccttc accggcataa caaccgggga
    13321 gaaacgtaaa aaatgagtag gggaatattc ctgccgggag ataaacaggg ggcatgatgc
    13381 tgagataccc ggtggctacc gcgaaacgat gcttcccgcg cgtacgcacg acagagaagg
    13441 gatgcttttc gggaggatga ggggaagaac cgtcgttctg ataattgcag agttatttac
    13501 ggggcagtta cggtggggca gcccgtgtcc ggtgggcagg cgacaggtat caccgatggg
    13561 ataagactgt ctgattggta ttaaatatat aagagagaga tcaaacacat gaaacacatg
    13621 agatcaaata catgagttca tatatgtgaa ataaacactg tgagtgtgat cagtgggcat
    13681 gatgagaacg cagtagcaac agattgcgtt gtacccacag ggctatccct gatagctggc
    13741 gtataacctg cgtatataaa acgtacctgc aacacacgtg aacagagtgt atttgagcgg
    13801 taagcgtatc cgacctggtg agtgtcagtc tgagataccg gttaagtggt ggcacgggag
    13861 taatgagttt ggggagggta ccagaatgcc agtctggtgc agtatgctga cagtatcacg
    13921 ttaattgcag cataaagtga tgagaggcta atggaagtta aaaccaaaga agactggctg
    13981 tatcagctcc gtcgttgttc atcccgggag acactggaaa aagtgatttc ccacacgcgt
    14041 tataaactta cccctgcgga gctgaaagct ttcaactctg cggtcgatca ctggctggca
    14101 gaactaacca tgaacaaact ttacgatcgt gttcccgctt cagtccggaa atatgtcacc
    14161 tgaacgttcg gtaatcacag cggaattgca ccaggtgtta tctgctctgt gtgattcttt
    14221 ttgggttttc tcaggttgtt aaaagctgaa tatgttcctg ttgtcggaaa ccaggataat
    14281 accgggaacg ttaagcagta tagcgacaac aaagaattcg aaatcaaaag ggaatggtgt
    14341 ccggaggcgt gggcggtaat gggcagcatt ccgtattatg agtaatcacg ttatcacaat
    14401 ccctgcacac cgttttgctt atcaccatca cgtgctcctc acagtcttat ccgtaaccgg
    14461 ctttttgcat taaaaaaaca acactgaaaa ttcagccgtc agtccggaga cgacagtcgg
    14521 gttatcacag tgtgcctgag acattgcccg gttggagatg acttcaggga ctctgcacat
    14581 tttttacagc gtggttattc cgtatgaacg aaaccatacg gagatttaac catgacatac
    14641 gagtacaacc ccttctggca acaacgtatc cgtgagacgg tactgcgcgc actggatgtt
    14701 catccccgtc tgacggcatt gcgggttgac ctgcgtctcc cggatgtacc ggcagcaact
    14761 gacgcagcag tgatatcccg cttcatcaat gccctgaaag cccgaatcga cgcttaccag
    14821 aagcgtaagc gctgtgaagg taaacgcgta cataccacaa ggctgcatta cgtctgggcc
    14881 cgggagttcg gggagaacaa aggtaaaaaa cactatcacc tgatactgct ggtcaaccgt
    14941 gatacctggt gtggtgcggg cgattaccgc gatccggggt cactggccgg gatgattaaa
    15001 caggcgtggt gcagtgccct gggggtggat gccggatgct atgccacact ggcgaatttt
    15061 ccgtccaggc cggtggtgtg gctggaacgc gatgatgatg ctggtttacg ggatgtgctg
    15121 aaccgtgctg actatctcgc gaaagagtat accaaagtca acggtaccgg tgagcgtaat
    15181 tttggctgta gccggaattg agtacgactg gcgcaccagt atccagtaaa accaaaagaa
    15241 tggactgttg tctggctgca acgatgtacc ttttctccta ctggttgttt tttacgccag
    15301 agcgccattg tgtgacaacc acagtgctgt aacaacaacc aacctgcttt acccaacccc
    15361 ggatccagat tttaactgaa ccgccattca cactctgacc acgcagcccg taccgggacg
    15421 gctgacgcct gcgtgtcgtc tggcggttga ggagtatgat tatgtacgca aaatccttta
    15481 ttgcatttga tggaaacgga cgtctgaccg gtgcccgtac cgcacagact gcgccatatg
    15541 atcgttatac ctgtcatctt tgtggcagtt cgctcagata ccacccgcaa tacgacactg
    15601 aacgtccctg gtttgaacac actgacgaag ggctgactga gcacggtcat gagtgccctt
    15661 atgtcagacc ggagcgcaga gaaatactgt tgattaaacg cttacagcaa tgggtaccgg
    15721 atgccttacc cgtggtgcgt aaagcccacc ggcactgcag acaatgtcac cacgattatt
    15781 atggggaacg ctattgtaca cactgtcata cagggcgttt cagtgaggag gcagtggcag
    15841 gatgaacggt ctgaaaacgg ggctggggat cacaccgggt gagcacatta tcagtgcggc
    15901 ctcagccctt agcagaaata tcaggcattg tttctgcctt tcctgtcgtg gtcggttgat
    15961 tttgcagaca gatgctcagg gggcgtggtt tgagcacgat ttacatgccc tgactgcgca
    16021 acagaaggcg gcttgcatgg tgctgaatcc ggaaaagtcc cacccttaca ttgaagatat
    16081 ggcgatgttt ttatcgccat taccaaccgt gctggagtgg cattgtgtga tgtgcgagag
    16141 gctttttcat gggaagaagt tctgtgaggc ctgtggtacg gggatttatt gcagagcaac
    16201 atgtacgaag tatgtttaca gataccagcc agatttgctc cgggacccgg agtgagggag
    16261 atgcgacata attacttgat catgccagtc atgcccctgc cggatccgga tgtggtccgg
    16321 cgctttgttc cgcggacggt ggttcactgg ctgtgtctgc tgatgatcct ctttatgcga
    16381 cattcagaca ggggagacat cgataaacat catcatctgc gggcaaatag ttacacagaa
    16441 tgatcattgc taccggttat ggcaaaaatt ggtgatgatc aggtgaacca tcggtgcttc
    16501 cgttgattct tatggactga taaagttttg tcagctgaat ctttatacgg aatgctcttc
    16561 agtatgcgta cacaaattga ctatctggcg gataaatact gttttacaga acggaacgaa
    16621 tccccccgtc ttcgtcggca gtggcaggat gttctggagg agtgccggca gacagaggcc
    16681 gggccagaag aacggctgcg tattgcactg ctgaatgtgg attacgtcac cagttttgag
    16741 ctgccttttc gcttgctgct tacccggaca ccacaactga ttgccgcgct gcgtgaagaa
    16801 tggggcatca gccagaaaaa tgtggtgttt aacgataaac ggtttggttg cgtgtacagc
    16861 ctgaaggcca gcctttctgg tgtgccggat acattccggt atcatctgtc tcaccgtatt
    16921 cgtcgcgtgg ttgggaatga aaacacatca ttgccatatc agcaggttgc ccgggaagtg
    16981 aaagcgcccc gtgaacggct gaagtatgca ctggaggcgg gtttactggt gactgcactg
    17041 gacggcctgt tctggtttgg cagtcagcgt attgcggctg atgttctgag gctgagaaag
    17101 gccggaatgc cggtggtgac cacgaccgtg gaggtgcacg ataacctgac aggaacaacc
    17161 cgcaaagtac cggcatacca tctctgatac aaccatcagg gggaatattg ctctggcagc
    17221 agcagtacga cattctttat actttatccc cgtgcctgaa aacatgtgct aaggaaacgg
    17281 gaatggcaag gtccgattat gatattatca atctgtctct gaaacatgaa ctgaatgagt
    17341 ggctgacaga gagaggttat gccggactgg cggataaccg gaaccgcctg gcagaggtgg
    17401 ttacccgcaa attgcaggac agcttttata taaacgtttc ctgggatgcg ctgaacacgg
    17461 catacagtga acacccggag tggttttccg ggcttgtctc cggggatgag aattaataag
    17521 tggattatgc tgccatcagg cagtgtgatt gccggcaggg atttcgtttt attctgtttc
    17581 ttcccggggg tcgggtttga attttcctgg aaagttgtac atcggtcgca tgcagggatg
    17641 cggatagccg tcccgcataa acaccacccg ggtttcctca acactggtca cggtgacaag
    17701 cgcaccatga tcgtctctgt atcgctgatt gattttcggt aatgggatat cttctgacat
    17761 agatttcaga acggtaacag ttaactaaat tttcatgtgg tagtactgaa tcaggagtct
    17821 ctgcctcctg atgtgttttg ttaatcttct gtttttacta aaaattccct cactccttct
    17881 ttcattgcat tttgttgtgc tcttccttca tatccttccc gttttccaga aatcacttca
    17941 atgatgctga atcgttgctc atcgtgattt tccatgacca gaatcaaaaa ctgaactttt
    18001 ctgacataac tggctgtatt aaaagcattc agtggaataa ttttctgcac ttttccatta
    18061 attatggtta taaattttgc aatgtgctgt tttt
//
LOCUS       DAZFDP010000097        17702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFDP010000097 DAZFDP010000000
VERSION     DAZFDP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(308..1309)
                     /locus_tag="ACHJ1G_003320"
     CDS             complement(308..1309)
                     /locus_tag="ACHJ1G_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4765117.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1416..1712
                     /locus_tag="ACHJ1G_003321"
     CDS             1416..1712
                     /locus_tag="ACHJ1G_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4765118.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1846..2271)
                     /gene="arsC"
                     /locus_tag="ACHJ1G_003322"
     CDS             complement(1846..2271)
                     /gene="arsC"
                     /locus_tag="ACHJ1G_003322"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4765119.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2284..3573)
                     /gene="arsB"
                     /locus_tag="ACHJ1G_003323"
     CDS             complement(2284..3573)
                     /gene="arsB"
                     /locus_tag="ACHJ1G_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4765120.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3627..3980)
                     /gene="arsR"
                     /locus_tag="ACHJ1G_003324"
     CDS             complement(3627..3980)
                     /gene="arsR"
                     /locus_tag="ACHJ1G_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4765121.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4489..4572
                     /gene="dinQ"
                     /locus_tag="ACHJ1G_003325"
     CDS             4489..4572
                     /gene="dinQ"
                     /locus_tag="ACHJ1G_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4765122.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4626..5978)
                     /gene="gorA"
                     /locus_tag="ACHJ1G_003326"
     CDS             complement(4626..5978)
                     /gene="gorA"
                     /locus_tag="ACHJ1G_003326"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4765123.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6050..6892)
                     /gene="rlmJ"
                     /locus_tag="ACHJ1G_003327"
     CDS             complement(6050..6892)
                     /gene="rlmJ"
                     /locus_tag="ACHJ1G_003327"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4765124.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7095..9137
                     /gene="prlC"
                     /locus_tag="ACHJ1G_003328"
     CDS             7095..9137
                     /gene="prlC"
                     /locus_tag="ACHJ1G_003328"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4765125.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9145..9897
                     /gene="rsmJ"
                     /locus_tag="ACHJ1G_003329"
     CDS             9145..9897
                     /gene="rsmJ"
                     /locus_tag="ACHJ1G_003329"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4765126.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9946..11415)
                     /gene="dtpB"
                     /locus_tag="ACHJ1G_003330"
     CDS             complement(9946..11415)
                     /gene="dtpB"
                     /locus_tag="ACHJ1G_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO4765127.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11581..11634
                     /locus_tag="ACHJ1G_003331"
     CDS             11581..11634
                     /locus_tag="ACHJ1G_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765128.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11733..12167)
                     /gene="uspA"
                     /locus_tag="ACHJ1G_003332"
     CDS             complement(11733..12167)
                     /gene="uspA"
                     /locus_tag="ACHJ1G_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4765129.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12558..12893
                     /gene="uspB"
                     /locus_tag="ACHJ1G_003333"
     CDS             12558..12893
                     /gene="uspB"
                     /locus_tag="ACHJ1G_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4765130.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13138..14637)
                     /gene="pitA"
                     /locus_tag="ACHJ1G_003334"
     CDS             complement(13138..14637)
                     /gene="pitA"
                     /locus_tag="ACHJ1G_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4765131.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14869..16071
                     /gene="yhiN"
                     /locus_tag="ACHJ1G_003335"
     CDS             14869..16071
                     /gene="yhiN"
                     /locus_tag="ACHJ1G_003335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4765132.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16387..17439)
                     /gene="yhiM"
                     /locus_tag="ACHJ1G_003336"
     CDS             complement(16387..17439)
                     /gene="yhiM"
                     /locus_tag="ACHJ1G_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4765133.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4620 a         4444 c         4531 g         4107 t
ORIGIN      
        1 atgttttaga tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt gttgtattag
       61 cgactgaaat ctgcctttta aaaaggagga gggtactgag acaaagagtc attgcttctc
      121 cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac ttgttgaccg
      181 agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg attatcctgt
      241 ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagc ttactgctgg
      301 catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata cctaatataa
      361 ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat atcacttccg
      421 tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga cccattcctt
      481 ttgtcatcag aacagatgcc agagggataa ctgcctcagc ccggatatac agcggaatac
      541 cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga gcggcaatcc
      601 acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac aaaagatatg
      661 gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg ggattaatac
      721 ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct ttagccgctg
      781 gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt tttgctggag
      841 cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac cccagagaat
      901 ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac aacaaggtga
      961 ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt aatggtgaaa
     1021 caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca
     1081 tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca gccaggagat
     1141 agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc accttttgtc
     1201 ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg acggcgagaa
     1261 acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata cgtaacctca
     1321 ttggataaat ctatatttcc agaataatcg aaatgaaatt cagatcaaga tatttctggt
     1381 attatcgaaa tatcaaccag acagaggggt gtcatatgca gttggaagaa gtcgctaaag
     1441 cgctgaaaga gctaggtcac ccgacccgtt tgttcatatt taaacatctt gtcaaagccg
     1501 gggaacaagg gttgccggtc ggtgagttgc aaaagcagct tgggatacct ggctccacac
     1561 tcagccacca tatatcagca ctggtatcgg taggactggt caaacaaaac cgtgagagtc
     1621 ggacgctgat gtgtgtttcg caatacgcaa tgctggaagc gataattgaa ttcttacgcg
     1681 aggagtgctg cgtaaatagc aaaattgcct gatgcgctac gctctacata tttcatgcca
     1741 tatattgaat ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg catccgacat
     1801 aaacaaagcg cactttacca actatcggca gtggatatcg ccgctttatt tcaggcgctt
     1861 acccgcttca tcaaccactt tctcgccatc ttccttggag aatgcgcctt tttgcgcatc
     1921 tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca gcggcgtcac
     1981 cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta accgatcgtc
     2041 agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt tacgcagcag
     2101 cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc gcgttggcgg
     2161 agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca tctccagcgt
     2221 attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca tatcagtatc
     2281 tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa acagcacagg
     2341 cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca tattcttctg
     2401 cgaaagtacg tgcagccaga gtagcgtagc caggctacca attggggtaa ttttcggtcc
     2461 caaatcgcag ccaatcacat tggcataaac catcgcttct ttgataacgc cagatgccgt
     2521 gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca taatagaaga
     2581 gaggaaggcg gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt tatccgccag
     2641 cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc cataaaccac
     2701 cagatacatg ccgagcgaga agatgacaat ctgccagggg gcaccgcgca ggactttacc
     2761 cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcagcgcgc ccacagctgc
     2821 aatggcgctc accggaatgc cgagcggttc caggacgaaa aatcccacca gcagaagcag
     2881 taaaacaacc cagccagttt tgaacgtagc aggatctttg atcgcttctg cgggagattt
     2941 cagcagcgcc atatcgtagt tctgcggaat atctttgcga aaatagagat gtaacatcac
     3001 cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcgt attcgcgaaa
     3061 gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa taagcggcag
     3121 gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtag tgcctttact
     3181 gaaccctaaa gccagcagca tggcgatgac aatcggtgtc aaaataagcg ccgcgccatc
     3241 attggcaaac agggcggcaa cggcagcacc gagcaggaca atccaggtaa acagcaagcg
     3301 accacgacca ttaccccagc gtgagacgtg cagcgccgcc cattcaaaaa agccggactc
     3361 atccagcagc aggctgatga taatgacggc gataaacgca gccgtcgcgt tccagacgat
     3421 attccacacc accggaatat cacccggatg gaccacgccc gtaactaacg ccagtactgc
     3481 gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa ccaatacgat
     3541 ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggataaa acacatctga
     3601 aaattcatat gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc cccggaacag
     3661 ttttgtcgag ccaggttgcg gacaatcgcc tgaacctttt cctgttcaca tcgccaggcc
     3721 tcatcaataa ttttcgccgc ccatgctgga atatgcggtg ataagcggta atgaacccac
     3781 ttaccttgct tgcggtccag caatagcccg ctttcacgca gcaatgccag gtggcgggag
     3841 atcttgggct gcgactggtc gagagcagtg cagagatcgc agacgcataa ctctcccagt
     3901 tcgctgagca gtaaaacgat gcccagacgg gtttcatcag caagaatttt gaacaattgg
     3961 atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc tctcttcgaa
     4021 gcggataagt caaaaacata tatgacttaa cgaatgtgta agtgcagagg aaggtaatag
     4081 gtgtgaattt tgagttggct attcatttga aaggaggtct gaatcaggac gtaaaaaaac
     4141 cgactttgcg tcggtttttt actttccagc cctgagttgg tggcactggc tggagtgaga
     4201 atgccattat ctaacgcatc aatgctaata ccatactgaa actattgcaa ggacatgctg
     4261 gttttataac ctgaatgtac tgtatgatta tccagttagc tctgaggcat tttcactctg
     4321 gtaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc cgttaactga
     4381 tgctctggcc ggagtgagaa tgccattatc taacaatagg gcatgcgccg tgacaggcag
     4441 tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt gattgataaa
     4501 gcaatcatcg ttcttggggc gttaattgcg ctgctggaac tgatccgctt tctgcttcag
     4561 cttctgaact gatagcggaa acgtaattaa gggctaagag cacactactc ttagcccttt
     4621 aacatttaac gcattgtcac gaactcttct gccgccgttg ggtgaatggc gacggtattg
     4681 tcgaagtctt ttttggttgc acccatcttc agcgccaccg cgaagccctg caacatttcg
     4741 tccataccaa agccaatgcc gtgaataccg acaatcttct cttccgatcc aacgcacacc
     4801 agcttcatgc ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag
     4861 gatttataca ctttcacctg atcgtcgcca tactgctcgc gcgcctgcgg ttccgttaaa
     4921 ccaacagtac caatcggcgg atggctgaag accacggtcg gaatgttgct gtaatccaga
     4981 tgctcatccg gcttgttatt aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc
     5041 accggtgtca gctccactgc acccgtgtta tcgcccaccg cgtaaatacc ttcaatattg
     5101 gtgttttgat atttatcgac gacgatatag cctttttcgt tagttttaac gccagcggct
     5161 tccaggttga tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc
     5221 gtttcactgc gaccatcttc cagctccagc gtcaggctac catcggcatt tttcactacc
     5281 gctttcggga tggcgttggt gtgcagctgc gggccttcgg cgttcatcac ttcgaccagc
     5341 gtttcggaaa tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac aaacagatgc
     5401 gttttcgcgc cgaggccgtt aatcacgccc gccagctcaa cggcgatgta acccgcgcca
     5461 acaaccgcca cgcgctctgg caaagcagga agggcgaaga agccatcaga atcaataccg
     5521 tattccacgc ccggaatatc cgggtggctc ggacgaccgc ctgtggcgat cagaatatga
     5581 tcggccgtga tggtttcgcc gtttacctcc agcgttttgg catcaacgaa gcgggcaaag
     5641 cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt atgaatacgg
     5701 tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaatttatt gatagtggta
     5761 tcaaaaccat aatccgggcc gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac
     5821 atcacttttt tcggcacaca gccaacattt acgcaggtgc cgcccagctc tttggcttca
     5881 atcagcgcac atttctggcc gtacatagcc gcgcggttga tggaggcgat accgccgctg
     5941 ccgccgccga tggcgatgta atcatagtgt ttagtcatga caaagtgtcc ttatcgttga
     6001 ttaccgcgat tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat tactccggca
     6061 cgatccagct tacggtggcg tgcccggtgc ctgccggaac cagtttgctg tgcagccacg
     6121 gcagcacgtt attcatctgc tgttccagtt tccacggcgg gttaatcaca atcatgccgg
     6181 aagcggtcat gccacggcga tcgctgtctg gcagtaccgc cagttcaatt tgcagaattt
     6241 tgcgaatacc ggtcgcttcc agatcgtgga tcatgcgctt aatttgctga cgcagcacca
     6301 ccggatacca cagtgcgtaa gtaccagtgg cgaaacgttt gtaaccttct gctatcccgc
     6361 tgaccaccgc ctgatagtcg gttttcattt catacggcgg gtcgataagg attaaaccac
     6421 ggcgggaaac cggcggcagt ttggccttaa gctgctggaa accatcggct ttttcgacgc
     6481 gcgcacggct gtctttctga aattcagaac gcagcaacgg gtaatcgctc gggtgcagtt
     6541 cggtcagttg caggctgtct tgttcgcgca gtagctggcg agcaatcaat ggcgaaccgg
     6601 ggtagtaacg caactgaccg ctacggttga agtgttttac cacattgatg tacgcctcca
     6661 gttctgcggg caaatcgtcc tgctgccaga tacgggcgat gccttcgaga tattcgccgg
     6721 tacgctcggc atgttcgctg cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat
     6781 agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga acggtatgtt
     6841 taaggacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc atgggtaaag
     6901 gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagcgta tcaggacagt
     6961 ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat tcactacact
     7021 taaccccatg ctacacacat tatgtaaagc gcctgttgag cgcttcctta acctctttaa
     7081 ccaggactgc gcgaatgacg aatccgttac tgactccctt tgaattgcct ccgttttcta
     7141 aaattctccc ggaacatgtc gttccagccg tgactaaggc gctgaacgac tgccgcgaaa
     7201 atgtggagcg cgtagtagcg caaggagcac cgtacacctg ggaaaatctc tgccagccgt
     7261 tggcggaagt ggacgatgtg ctggggcgta tcttctcccc ggtcagccac ctgaactcgg
     7321 tgaaaaatag cccggaactg cgtgaagcgt acgaacaaac cctgccgctg ctgtcggaat
     7381 acagcacctg ggtagggcaa catgaagggc tgtataaagc gtatcgcgac ctgcgcgatg
     7441 gcgatcatta cgccacgctg aacacggctc agaaaaaagc ggttgataac gcactgcgcg
     7501 acttcgaact ctctggcatc ggtctgccaa tagagaaaca acaacgctac ggtgagattg
     7561 ccactcgtct ttccgagctg ggcaaccagt acagcaacaa cgtcctcgat gcgacgatgg
     7621 gctggaccaa actcgttacc gacgaagcgg agctggcggg gatgcctgaa agcgcgctgg
     7681 ctgcggcaaa agcccaggcc gaagcgaaag agctggaagg ctacctgctg acgctggata
     7741 tcccaagcta cctgccggta atgacctact gcgacaacca ggccttgcgt gaagagatgt
     7801 atcgcgctta cagcacccgc gcctccgatc aaggcccgaa cgccggtaaa tgggataaca
     7861 gcaaggtgat ggaagagatc ctcgctctgc gtcacgaact ggcgcaactg ctgggctttg
     7921 aaaactacgc ctttaaatcc cttgccacta aaatggcaga aaacccgcag caggtgctgg
     7981 atttcttaac cgatctggca aaacgcgcgc gtccgcaagg cgaaaaagag ctggcacaac
     8041 tgcgcgcctt cgccaaagcg gaatttggcg tcgatgagtt gcagccgtgg gatatcgcgt
     8101 actacagcga aaaacaaaaa cagcacctct acagcatcag cgacgagcag ctgcgtccgt
     8161 acttcccaga aaacaaagcg gttaacggcc tgtttgaagt ggtgaaacgt atttacggca
     8221 tcaccgctaa agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac
     8281 tgtatgacga aaataacgaa ctgcgcggca gcttctacct cgatctgtat gcccgtgaaa
     8341 acaagcgcgg cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa gctgatggtt
     8401 cgctgcaaaa accggtcgcg tatttgactt gtaacttcaa ccgcccggta aatggtaaac
     8461 cggcgctgtt cactcacgag gaagtgatca ccctgttcca cgagttcggt cacggcctgc
     8521 accatatgct gacccgcatc gaaaccgctg gtgtttccgg tatcagcggt gtgccgtggg
     8581 atgcggtcga actgccgagt cagtttatgg aaaactggtg ctgggagccg gaggcgctgg
     8641 cgtttatctc cggtcactat gaaaccggcg aaccgctgcc gaaagagttg ctggataaaa
     8701 tgctggcggc gaagaactac caggcggcgc tgtttattct gcgtcagctg gagttcggcc
     8761 tgtttgattt ccgccttcat gccgagttcc gcccggatca gggggcaaaa atcctcgaaa
     8821 ctctggcaga aatcaagaaa ctggttgccg tggtgccatc tccgtcctgg ggccgtttcc
     8881 cgcacgcttt cagccatatt ttcgccggtg gttatgcggc aggttactac agctacctgt
     8941 gggctgacgt actggcggca gatgctttct cgcgctttga ggaagagggc attttcaacc
     9001 gtgaaaccgg gcagtcgttc ctcgacaaca ttctgagccg tggcggttca gaagagccga
     9061 tggatctgtt caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg ctggagcatt
     9121 acggcattaa gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga
     9181 gacggcgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga agacaacctg
     9241 atggcgctgg tgttaacgcc ggaacatctg gaattgcgca agcgtgatga gccaaaactt
     9301 ggcggcatat ttgttgattt tgttggcgga gcgatggcgc accgacgcaa attcggcggt
     9361 ggtcgcggtg aggcggtggc gaaagcggtg ggcattaaag gcgattattt gccggatgtg
     9421 gtggacgcca ccgcagggct ggggcgcgat gcctttgtgc tggcttcagt cggttgccgc
     9481 gtgcggatgc tggagcgtaa tccagtcgtt gccgcattgc tcgacgacgg cctggcgcgt
     9541 ggttatgcgg atgcggaaat cggcggctgg ttgcaggagc gtttgcagtt aattcacgcc
     9601 tccagcctga cggcgctaac cgatattacc ccacgcccgc aggtggtcta tctcgacccg
     9661 atgttcccgc ataagcagaa aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg
     9721 ctggtggggc cggatcttga tgccgatgga ttgctggagc ctgcacgctt gctggcgacc
     9781 aaacgcgtgg tagtaaagcg cccggactac gcgccaccgc tagcgaatgt cgccacgcca
     9841 aacgcggttg tgactaaagg gcatcgcttt gatatctatg caggtacgcc cgtgtaaaag
     9901 taaagcaggg tagcgataac gctaccctgc tttctgcaag aataattaat ggctttccgg
     9961 cgtcgcaatc atgcgtttca gccacggcac catcaacagc atcactactg caacgcccag
    10021 cgtgaccaga ccaatcttac caaacacgtt ggtatagacg ggcaacgtct caagcggatc
    10081 ggtaatgttg tccggcaccg cggtaaatgt tgccacatag ccgcccagca agaacgcggc
    10141 agcctgcgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg gcaccagggc
    10201 agcaatcatc gccaggccaa gggcgctaat aaacagttca cctaagctct ggaataagta
    10261 caccagcacg ataaaccatg gcgatgtcag cccttgtgca tccgcaaacc acattcccgc
    10321 agctgccgcc gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa atttcatcgg
    10381 catcgagagg tctttgcctt tgttacccag atgcgtgtaa atgcctgcca gtattgggct
    10441 ggcgagtacc acccagaacg ggttaagcgc ctggaagctg accgggttga tggaaaaacc
    10501 gagaatttca tgatgcacgt tgttgatggc aaagaagttc agcgatgttg gcatctgggc
    10561 gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa acattttatt
    10621 gcgcccggtt ttatccagct tgaatgcctg acgaaagaag atgatggtga cgacgatgga
    10681 gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccatg cacatacgaa
    10741 gatcatcacc acgctgccaa gtaatacgta caacagtttg ctgaagctca tcggcttgaa
    10801 gtcgggttca gaaccaatgt ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc
    10861 gataattaac cccgccccgc acaggttgta ggtgactgaa taaccgaatc tatcagcgat
    10921 cacaggggcc agcgataacg ctatcaacga gccgatattg atcgacatat agaacagggt
    10981 gaatgcgcca tcaagccgcg gatctttcgg cggatagcac ttcgaaagca agctggctgg
    11041 gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga aaatcaggtc
    11101 aggcttaagt agcgacatgc cggtcatgaa gtagccaatc gccagcacaa gtgctccgag
    11161 aacaatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag
    11221 gccatagacc agcgcagcaa aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc
    11281 aagctgttta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc
    11341 ccataactcg acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg
    11401 tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa
    11461 gggataacgc gcgaaaaagt ttacatctgt cagagattct taatattgat gcggtgaata
    11521 gattaataca ctattgcaac agaaaataac cagctgatca cactaatgat ggaaaatatg
    11581 atggtgttaa atgttgtatt ggcaggagat tttgattgcg tacgcagatc gtaggccaga
    11641 taaggggttt acgctgcatc aggtaacaag gcgcgccaaa aagcaaaacg cccgcatatg
    11701 cgggcgttca ggaacacgtc gtggagggat gattattctt cttcgtcgcg cagcggaaca
    11761 atcagcatat caacgtgaac ggtgttgatc agctgacgtg cggaagacat cagtttgctc
    11821 cagaagtcct ggtggtgacc acaaaccacc agatccatat cgtatttctt gattgcatcg
    11881 accagaacct ggcccaggtc gccgctgccg ctcagggttt cagtgattgg gtagcctgca
    11941 ttagtggaaa gctcggtcag cgcatgatgt gtctcttcag agatgcgttt ctgcatatca
    12001 cccagattca catcaataag cccggtgtat aggtcagagt agtttacatc tacgtggatc
    12061 agagaaactt tcgcattgta ggggcgagcc atagagactg ctttctctac cagaactttg
    12121 ctttccgggg agagatcgac tgcgatgaga atgtgtttat aagccatagt gttactcctt
    12181 ccataaagtt gtcgatgact ggccagctag cgtttcttgt gccgttacgt cacccgcgtc
    12241 ctgcgaacta cgtgcgcggg gctaacccag ccgtaaagat tcagtgttaa gaccatcctt
    12301 accttatagc gacccggatg atccgtcaat ccgccttgct tacccgttag ataaacaaat
    12361 tttcgacatg aatatattga tagtggttaa ccttctggaa aaaaaacaac ctgatctcct
    12421 acactatcta tagagccgct cggatgttgg tcaccacaaa agcggtttct ccaggacctg
    12481 gtgttaactg attggcagcg tatcgggagc ggtagccccg gggtggaact ccgcgggcag
    12541 gtcgccgggg aggaggtatg ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt
    12601 gcattgttaa catggcgcgc tatttctcat cactacgcgc gttgttagtg gtactgcgta
    12661 actgcgatcc attgctctat caatatgttg atggaggggg cttttttacc tcacatggcc
    12721 aacccaacaa acaggtgcgt ctcgtttggt atatctatgc ccaacgttat cgcgatcatc
    12781 acgatgatga atttattcgc cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg
    12841 cattgtgtgg tctggtggtg gtcagcctga ttgcattgat gatttggcat taagcacttc
    12901 agcaataata acgcataaaa aaagcgggcc agtaaactga cccgctcgag actgattaca
    12961 aacgcaaaat tgcctgatgc gctacgctta tcaggatcta cataatctct gcaatctatt
    13021 gaatttgcat gtttttgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag
    13081 cgcactatgt cacaatctga agcgggccag ctgacccgct cgttttggtg tgtacgatta
    13141 caggaactgc aaggagagcc agtacagtcc gccggaaagc agtaccgcag ccggaagggt
    13201 aaacacccag gccatcagaa tactggttac ggttttacgc tgtaagccgc cgccatctac
    13261 caccatcgtc cccgcgacag aagaggagag tacgtgagta gtggaaaccg gcatcccggt
    13321 ataactcgcc aggccgatag acactgccgc cgtcatctgg gccgacatcc cctgagcgta
    13381 ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt gccacacggc gccagccaat
    13441 catcgtaccg atacctaacg ccagcgcgac cgccatgatg atccacaccg gtgcatactc
    13501 gatggtgcta agcatgtcgg acttcagttt cttcaacagg cgctgatcgt cagcactcac
    13561 accaggcatc ttcaccactt tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat
    13621 ctggctacgt tgatcaagcg acagcttgtc gtagctttcc acatcggtag tcaacatgcc
    13681 tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg caatggaact ccgcgggttg
    13741 cgttgcgcca gcttccggag ccggtactaa ctgatcagca ccggtagcct gtttgagcag
    13801 cgcaggatgc tgctcaaagt aagcttcgac gttgttgatg gcatcacggg tacgggtgat
    13861 ttcgtagcca gtggcattca tgttcaccac gaagcctgct ggtgcgacgc caatcaatac
    13921 caacataacc agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc
    13981 gatagcggaa aggatcagcg caatacgcgt ccagaacggc ggctttttct tgccgtcttt
    14041 cttttcacgc tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc cgctccagta
    14101 gcgacgcagc aagaaaatca gaccgccagc aaacaccagg ccgacaatag gggaaacgat
    14161 cagagaaccg aaaatactta atactttcgg gatattgagt gcatccacca ctgacgtccc
    14221 ggtcatcaac gcattggtta aaccaatccc gatgatcgcg ccaatcagcg tatgagagct
    14281 ggatgcaggt aaaccaaagt accaggtacc caggttccag ataatcgccg ccagcaacat
    14341 agagaacacc atggcaaggc catgagacga tcccatatta agcagcagat ccgtcggcag
    14401 catatgcaca atggcatagg caacactcag accacccagc aaaacaccca aaaagttgaa
    14461 caccgctgcc ataaccacgg cgagctgaga acgcatcgcg cgggtataga taacggttgc
    14521 cacggcgttg gctgtgtcat ggaaaccatt gatggcttcg tagaacagca caaaagccag
    14581 tgcaagcaat aataacagcc cggtatgcaa atccaggcca gcaaacaaat gtagcatagg
    14641 acgttacgcc attttgagga catgaacgcg gcgcattatc agtgactttc gcggcgcggg
    14701 caaagtgaaa tatagacttt ttttgatttg cctcctgtat ggatttcact caaaaaataa
    14761 ttatcttata taattcaggc aaatacttcc ttttagtaat attgatgctg gtgcgaccac
    14821 tgaggaatct ttacaattca cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt
    14881 gatgccatta ttataggcgc tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag
    14941 gcaggacgcc gggttctgct gatcgataat ggtaaaaaac cagggcgcaa aatccttatg
    15001 tctggcggtg ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc ttatctgagc
    15061 cagaatccgc atttttgtaa gtctgcactc gcgcgtttta cccagtggga tttcattgat
    15121 ctggtcaata aacacggcat cgcctggcac gagaaaacgt taggacaact cttctgcgat
    15181 gactccgcgc agcagattgt cgacatgctg gtggatgagt gcgagaaggg caatgtaacc
    15241 ttcagattgc gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt cacgcttgaa
    15301 ctgaacggca tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg gctgtcaatg
    15361 ccggggctgg gcgcgtcgcc gtttggttat aagattgccg aacaatttgg cctcaacgtg
    15421 ctgccgaccc gcgcgggtct ggtgccattc actctgcata aaccgttgct cgaagagtta
    15481 caggtgctgg cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg catcgttttc
    15541 cgtgagaact tactcttcac ccatcgcggc ttgtctggac cggcggtgtt gcagatttcc
    15601 agctactggc aaccgggtga atttgtcagt atcaatctgc taccggatgt ggatctcgaa
    15661 accttcctga atgagcagcg taacgcacat ccgaatcaga gcctgaaaaa cacactggcg
    15721 gttcatctac cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt
    15781 tcgctaaaac agctcaacgt gcgtgaccaa caggcactga ttagcacatt gaccgactgg
    15841 cgcgtacaac ccaacggcac tgaaggctat cgcactgccg aagtgacgct cggcggcgtg
    15901 gacaccaacg aactctcttc acggacgatg gaagcgcgca aagtgcctgg gctgtacttc
    15961 atcggcgaag tgatggacgt taccggctgg ctggggggct ataacttcca gtgggcgtgg
    16021 tcgagtgcgt gggcttgtgc gcaggatttg attgcagcga agtcgtcctg acctgttacc
    16081 taaagatgaa aattattaga gccgcattaa aaatgagcta attttgatag tggctatctt
    16141 gtgattattt tctaatgagc ccgtgaactg aaaccctcca ggcttaatat aaggtggaag
    16201 gaaaggtgat tgaaaactca cccagtggcc agatctttat aaaatatgaa caggttgaaa
    16261 aaaacagtaa ctttgttgtt tttttatcct taaccactat gcattaatgc tgcgttattt
    16321 catgatgcct aagaaaaacc agcgttacgc aaatggtcaa cgctggtttt atccggtacg
    16381 ttgcaattat tttttagcag aacctgcttc taatattgaa acgattgaga acaacgtaaa
    16441 gcataccgct cctaaaccga ccaaaacccg cgaaggaata aaatatccca tgtcggtctg
    16501 cgccatttcc gcaagaaacg atgccaggaa aaggcaaaac aggcaggtga agacgggaat
    16561 catcggtatt ctgttggcta acgaacaggt acgtctccat accagtgcca gtagccagac
    16621 ttttgagaat atgctgtaac agatcattcc gaggcaaata agaataatgc cgggagccag
    16681 tcgggcgctt gcatcggaac tgactaagac gtatatcccc tgcagtaccg tgattgagcc
    16741 gagaaaaata acccaataac accacagcca gtgttcttta gttgaaaacg tattacgtgt
    16801 ttgatggaca attgtggcaa caaggccaat tagacaggca cagattgcgg ttaacccaag
    16861 caatacgtga cccgcgacat aatgcggagt tatgtcagca ctacgtaaca gcgtaataga
    16921 ccaaatgaaa ccgagcaggg tgagtaaaac gggaacggca atgaggcaat taccgattaa
    16981 tgaagaataa gcctgtaccg gcgttccatc gctcttgctc cccgctgcat ttttgggaat
    17041 gagcagaaag tgaccggatg acgctgccac cgtcgataca caggcggcaa tcataccaac
    17101 gccgaaaata acatggccgg cgacaaactc gtctgccatc acatcattgc ctgctaacag
    17161 tgcccaaccc catatcatgg tgataattga ccccgcatag ccaataatgg ggaacaatgt
    17221 attgtaaaac gtattaacgc cgcgcgtgag ctgcgaaatg ataataaatg cggtagtgaa
    17281 taatgccagg catatggctg ccagagaaat cagcacatga cccgcaacga aatactcact
    17341 ttgcccagaa ctttccagca caaagccacc taacgcgata caaattaagc ccataattaa
    17401 agggattaat ttgaaaagcc acccgatata tatgttcact ttaaactcct caggtatccc
    17461 tgctaatatt gaaatgtgca ttaatgataa tttcagcctg ttaatataat gacgagatat
    17521 cattaaagac aaatgcatag cataactata gcgggattta ctttgcagag cgctactttt
    17581 cagcgttcaa aaggccatgg taaataaata tttatcctaa gattaattct tattctgttg
    17641 ttttttacaa ttatcttcca gggagggatt ctttttcatt atttggtaaa agaccggatg
    17701 gt
//
LOCUS       DAZFDP010000098        17701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFDP010000098 DAZFDP010000000
VERSION     DAZFDP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..487)
                     /gene="ybcV"
                     /locus_tag="ACHJ1G_003337"
     CDS             complement(77..487)
                     /gene="ybcV"
                     /locus_tag="ACHJ1G_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO4765134.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            788..994
                     /locus_tag="ACHJ1G_003338"
     CDS             788..994
                     /locus_tag="ACHJ1G_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765135.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1567..2094
                     /locus_tag="ACHJ1G_003339"
     CDS             1567..2094
                     /locus_tag="ACHJ1G_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4765136.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            2103..4202
                     /locus_tag="ACHJ1G_003340"
     CDS             2103..4202
                     /locus_tag="ACHJ1G_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4765137.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            4199..4411
                     /locus_tag="ACHJ1G_003341"
     CDS             4199..4411
                     /locus_tag="ACHJ1G_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765138.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4411..5919
                     /locus_tag="ACHJ1G_003342"
     CDS             4411..5919
                     /locus_tag="ACHJ1G_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4765139.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5864..7891
                     /locus_tag="ACHJ1G_003343"
     CDS             5864..7891
                     /locus_tag="ACHJ1G_003343"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4765140.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7978..8301
                     /locus_tag="ACHJ1G_003344"
     CDS             7978..8301
                     /locus_tag="ACHJ1G_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO4765141.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8294..8569
                     /locus_tag="ACHJ1G_003345"
     CDS             8294..8569
                     /locus_tag="ACHJ1G_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4765142.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8581..9159
                     /locus_tag="ACHJ1G_003346"
     CDS             8581..9159
                     /locus_tag="ACHJ1G_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4765143.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9156..9557
                     /locus_tag="ACHJ1G_003347"
     CDS             9156..9557
                     /locus_tag="ACHJ1G_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4765144.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9568..10311
                     /locus_tag="ACHJ1G_003348"
     CDS             9568..10311
                     /locus_tag="ACHJ1G_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4765145.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10372..10758
                     /locus_tag="ACHJ1G_003349"
     CDS             10372..10758
                     /locus_tag="ACHJ1G_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4765146.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10767..11096
                     /locus_tag="ACHJ1G_003350"
     CDS             10767..11096
                     /locus_tag="ACHJ1G_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4765147.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11068..14133
                     /locus_tag="ACHJ1G_003351"
     CDS             11068..14133
                     /locus_tag="ACHJ1G_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4765148.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14133..14462
                     /locus_tag="ACHJ1G_003352"
     CDS             14133..14462
                     /locus_tag="ACHJ1G_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4765149.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14472..15170
                     /locus_tag="ACHJ1G_003353"
     CDS             14472..15170
                     /locus_tag="ACHJ1G_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4765150.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15175..15918
                     /locus_tag="ACHJ1G_003354"
     CDS             15175..15918
                     /locus_tag="ACHJ1G_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4765151.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15816..16463
                     /locus_tag="ACHJ1G_003355"
     CDS             15816..16463
                     /locus_tag="ACHJ1G_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4765152.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16524..>17701
                     /locus_tag="ACHJ1G_003356"
     CDS             16524..>17701
                     /locus_tag="ACHJ1G_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4765153.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT         4274 a         4082 c         5298 g         4047 t
ORIGIN      
        1 taaatcacaa ttgttgatgg tttttattaa accaatgcag tccggctcag gagtgagaga
       61 agccggacgt tatggtttag cgtggtaaga tctgtgtagt tttctggatg ctttcagtaa
      121 atagtaatga gttatcaaag gcatagtaat atctttggtg ttcctggata tttgtaaccc
      181 atcggaaaac tcctgcttta gcaagatttt ccctgtattg ttgaaatgtg atttcttttg
      241 atttcaactt attataggag gtctctataa gatgtttgtt tctggagaat ttaacattta
      301 caaccttttt gagtcctttt actaacacta tgttgtcgtt ttctaacaca atgtgaatat
      361 tatctgtggc taaatagtaa atataaagtg agacattgtg acgttttagc tcagaataaa
      421 ataattcaca gtttaaatct ttacgcactt gatcgaatat ttctttaaaa atggcagcct
      481 gagccattgg taaaccttcc atgtgatacg atggcgcgta gttagcatta tcgtgtttat
      541 tgtttcaatc tggtctgacc tctttgtgtt ttgttgatga tttatgtcaa atattaagcc
      601 tgtttttaat gaatagtgtt gattgcgtaa caaaattgag ccttgctggc atccaggagg
      661 gatatgcaac cgacagatgt atgtaaggtc gatgtactca aactttcata cttttcctct
      721 tttatgcagt aagatttgaa gtaatatttt aaccgctaga tgacgagcaa acgcatggag
      781 cgacaaaatg aataaagaac aatctgctga tgaagtctcg ttggatctga ttcgtgtaaa
      841 aaatatgctt aatagcacca tttctatgag ttacccggat gttgtaattg catgtataga
      901 acatcaggtg tctctggaag cattcagggc aattgaggca gcgttggtga agcacgataa
      961 gaattcgaag gattattccc tggtggttga ctgagcacca taactgctaa tcattcaaac
     1021 tatttcacct gtgacagagt caatatcgca ttctgtcact gtcaggctaa tacagagctg
     1081 caattcaact actgcaatgt cctcgtaatt aggtgaattt acaatatcgt cctgttcgga
     1141 tgccggttgc attgctgaag atgaggcatt tatggttcgc atattttccc ctcatgctcg
     1201 tcagtcctgt gcgtaggaag aaacaggaca ctcacactaa tttgtgtggg catgctgtga
     1261 tgtccttctg aattattcct atgccattat gtaaagcgct gtatcagatg ctcatcacgg
     1321 ctgtcaggct gtcaggctgt cgggtcctcc cggtgggggc ccctgccacg gggcgggagc
     1381 gtcgcggaaa aaggctagtt tttgcatttc catggcggcg gcagcatgtt tggtaattta
     1441 ttgataatta aaagttattt ctcttttcac ctgtacaata tttctttctc cctgtcatta
     1501 gaccagtttg caattaattg aaatatataa ataaacatga ttttcacctg ccagatggag
     1561 ttgcttatgt caaatgtgag cgggatcggt gatgcttatt actggagtgt ttttaaaatc
     1621 gctgaggcct ttgggcttca ccgggacaca gtaaaaaaac ggctcctcgc ggctaacact
     1681 cctgtggctg cgactgtcag ggggaacccc gtttacgccc tgcagcatgt cgggccagcc
     1741 ctgtttagtg tgaagcatga ggcagcagac tctgttcatg atccatcccg tatggagccg
     1801 aaagagagaa aggactggta ccaatctgaa aatgaaagga tcaagctgga aaaggagcag
     1861 cgaaaactca tccccgttga tgaagtagtc atcgtctatt cgtccatgag aaaggctgtc
     1921 gtccaggttc tggagacaat tccggatgtt cttgaacgcg attgcgccct gactcctcag
     1981 gccgtcggcg ttgtacagca ggccattgat gacctgcgat acactcttca ggaaaaatcc
     2041 tacgaggctt gtgctgctga attaattcct gatgaggaag gagagagtct ctaggaggaa
     2101 taatgggttt ttcatcagcc cgaaatttgg gaagggacat atcggcagga ttttccccac
     2161 cacgtcgcat gccgatttcg gaggctgtta aaaaattcat gcgtgttccc aagggggctg
     2221 gtaactcggt gccatgggat cctgaactga caccctacat cattgagccc atgaactgcc
     2281 tggcatcgcg tgaatacgat gcggtgattt ttgttggtcc tgcgcgaaca gggaagacca
     2341 ttggtctgat cgatggatgg attgtctata ccatcgtttg cgatccttcg gacatgctcg
     2401 ttgtgcagat gaccgaagat aaggcccgcg agcattctaa aaagcgcctc gacagaacgt
     2461 tcagaagcag tgcggcggta aagaaaagaa tgagtccacg tcgtaacgac aataatgtcc
     2521 atgataagac gttcagggat ggctcgttcc ttaaaattgg ttggccctcg gtcaacatta
     2581 tgtcgtcgtc ggattaccgg tttgtcgcct taaccgatta cgaccgtttt ccggagaata
     2641 tcgacagcga gggtgatggt ttctccctgg cctcaaaacg taccaccaca tttatgtccg
     2701 ctgggatgac tctggtggag agctcgccgg gacgtgacat ctgcgacagc aaatggcgac
     2761 gtaagtcgcc tcatgaagcg ccaccgacga ctggtattct ttccctttac aatcgtggtg
     2821 accgccgccg ctggtactgg tcatgtccgc actgcggtga atattttcag ccagccatgg
     2881 atgccatgac cggctaccgt aatgaaccgg atccctttaa agccagtgag gcggcgtatc
     2941 tactttgccc gcactgcagc ggcattatca ctgcggagaa aaagcgtgag ctcaatagtg
     3001 caggagtctg gttgcgtgaa ggtcaggtca ttgatcgtaa cggcaacgtt tccggtgaac
     3061 cgcgccgctc ccgtatcgcc agtttctgga tggaagggcc agctgctgcg tatcagacct
     3121 gggcgcaact ggtttacaaa ttactgactg cagaacagga gtatgaagcg acaggaagcg
     3181 aagaaacact cagggcggtt atcaacaccg actggggatt gccttatctt cctcgtgcca
     3241 gcatggagca acgaaaaagt gaactgcttg agcagcgggc agagccagtt ccttcccgca
     3301 gtgtgccgga tggcgttaat ttccttgtgg cgacagtgga tgtgcaggcg ggacgtcatc
     3361 gccgttttgt ggttcaggta acgggctatg gcagtcgtgg cgaacgctgg attattgatc
     3421 gttacaacat cacgcagtca ttgcgcagtg actgcgacgg ggagagccag cgaattgatc
     3481 cggccagcta tccggaagac tgggatgtcc tgctgacgga tgtttttcat aaaagctggc
     3541 cgctggcctc cgatccttct caacaaatgc gactgatggc aatggcggtg gactccggcg
     3601 gtgaagacgg ggtcactgat aatgcctata aattctggcg tcgttgccgt cgtgatggcc
     3661 ttggtaaacg tatttacctg tttaagggtg acagcatccg gcgcgcaaaa ctgatcagcc
     3721 gtacattccc tgataacacc ggacgaacgg gccgccgggc gcaggccgca ggtgatgtgc
     3781 cgctctggct tcttcagacg gatgccctga aagaccgggt gaataacgcg ttatggcgtg
     3841 actcgccagg tccaggctat gtgcatttcc ctgactggct ggggagctgg ttttacgacg
     3901 aactgacgta tgaagagcgg agcagtgacg ggaaatggag taagccgggt cgcggtgcca
     3961 acgaagcttt tgacctgatg gtgtatgccg aggctctggt cattctgcat ggatacgaaa
     4021 agatccgctg gccggatgca ccggagtggg cgagccggga gacctggctg gagtgtgtcc
     4081 aggacagtac cgaaccgtca tcctcaccgg aaccggtatc cacgcctgtt aaaaaacaaa
     4141 aacggaagaa aacagtaact gacgatgtta acccctggct gacttccgga ggatggttat
     4201 gaaccagaat gatatcgaag ccatgattca gcgttatacg gaagctgaaa tggcggtgct
     4261 ggacggaaaa tccgtcacct ttaatggtca gcagatgacc atggaaaact tatctgagat
     4321 ccggcaggga cggcaggagt gggagcgccg ccttgcggct ctgattacac gacgacgggg
     4381 gcatcccggg taccggctgg cgaggttctg atggcaattc ttgatgatgt gattggcgtt
     4441 ttttcaccag gatggaaagc ggcaaggctg cgttcccgtg cggtgatcca ggcttatgag
     4501 gccgtaaaaa cgacgcggac acacaaagcc cgacgggaga accgaactgc cgaccagtta
     4561 agccagtacg gggccgtgtc gttacgtgag caggcccgtt accttgataa caaccacgat
     4621 ctggttattg gtgtatttga caagctggaa gaacgggtgg tggggaaaaa cgggattatt
     4681 gtcgagccac atccggtatt acgcaatggg gccattgccc gtgacctggc tgcggagatt
     4741 cgcacccgat ggagtgaatg gtctgtcagc ccggaagtca ccgggcagtt tacccgtccg
     4801 atgctggaac gtctgatgct tcgtacctgg ctgcgcgatg gtgaggtgtt tgcccagatg
     4861 gtttccgggc gcataaacag cctggcgcct tctgccggtg ttcatttctg gctggaggcg
     4921 ctcgagccgg actttattcc catgactagt gatgagagca acaggctgaa tcagggcgtg
     4981 tttgttgatg actgggggcg tcccgaaaaa tatctggtgt ataaaagccg tcccgtatcc
     5041 ggacggcaga tggaaaccaa agaagtggat gcagagcgaa tgctgcatct taaatttgtt
     5101 cgccgtctgc accagatgcg cgggacgtct ttgttgtccg gtgtgctgat ccgcctcagt
     5161 gccctgaaag aatatgaaga ttctgagctg actgcagcaa ggatcgccgc tgctctgggg
     5221 atgtacatcc ggaaaggcga cgggcagagc tatgaaccgg atggtaatgg cagaaaggag
     5281 aatgaacgtg agcttaccat tcagccgggc attatttacg acgatctgaa acccggcgaa
     5341 gaaatcggaa tggtgaagtc ggatcgcccc aatcctaacc ttgaaacttt tcgtaatggt
     5401 cagttgcgtg ccgtggcggc gggcagtcgt ctgagttttt ccagtacagc gcgcaactat
     5461 aacggcactt acagcgccca gcgtcaggag ctggttgaat ccactgatgg ctacctgatc
     5521 ctgcaggact ggtttattgg tgccgtcacc cgcccgatgt atcgtgcatg gctgaaacag
     5581 gctgtggcat ccggtgttat caggctaccc cgcgatcttg accgttcttc actgtatacc
     5641 gcggtgtatt ccggaccagt gatgccgtgg attgaccctg ttaaggaggc tgaggcctgg
     5701 aaaatccaga ttcgtggtgg agcggcgaca gaatcagact gggtacgtgc tggtggtcgt
     5761 aatccggatg atgtcaaacg tcggcgcaag gccgaaattg atgaaaaccg caagctggat
     5821 ctggtatttg ataccgatcc ggccagtgat aaaggaggca gtagtgccgc aacgaaacga
     5881 caggagccgc agcacaccga cgaccagtcc gaagaataat tcctggttca ggatgcaggc
     5941 tggtcaccag agtgacgcgg atatttatat ttatgacgag attggtttct ggggtgttac
     6001 agcgaagcag tttatcagtg atctgaatgc actgggcgat atcacccaca ttaatctcca
     6061 tattaattca ccgggtggcg atgtctttga aggcatcgcc atttttaatg cgctgaaaac
     6121 acatggtgcg tccattaccg tttatgtcga cggtgtggcg gcgtcaatgg cgtcggtcat
     6181 tgcgatggta ggaaacccgg tcattatgcc ggaaaactcc ttcatgatga ttcataaacc
     6241 atttggcttt acgggcggtg atgcggagga catgcgcacc tatgccgacc tgctcgataa
     6301 agttgaggcg gttctgttac ccgcttatgc acagaaaacc gggaaaacca ccgatgaaat
     6361 tgctgccatg ctggcggatg agacctggat gtccggtgcc gaatgtctgg cacagggatt
     6421 tgctgatcag gtgacaccag ccgttaaggc aatggcatgt attcagtcaa aacgtacaga
     6481 ggaatttaaa aagatgccgg aatccattcg aaatatgatt actccgccac gcaacagtgc
     6541 tccacgcgta caggatgatg gacctgcagc ctcccggacg ccagtgcagg cagcagcacc
     6601 cgtggtggat gaaaacagca tccgtgcgca ggtcctggca gagcaaaaag cgcgtgtaaa
     6661 cggtattaat gatctgtttg ccatgtttgg cgggcgttat cagtcgttgc aggctcagtg
     6721 tcttgccgat cctgaatgtt cgctggagca ggcccgcgaa aagctgttga acgagatggg
     6781 gcgcgagtcc acgccatcca ataaaaatac cccggctcat atttatgccg gtaacggtaa
     6841 ttttgtgggg gacgggatcc gccaggcgct gatggcgcgc gccgggtttg aaaaaactga
     6901 acgtgataat gtctacaacg ggatgaccct gcgtgaatat gcccgtatgt cactgactga
     6961 acgtggtatt ggtgtttcca gttataaccc gatgcagatg gtcggtgcgg cgttcacaca
     7021 cagtacgtct gacttcggta atattctgct ggatgttgcg aacaaagcca ttctgcaggg
     7081 ctgggaagat gcccctgaaa cctatgaaca gtggacgcgg aaaggtcagt tgtctgattt
     7141 taaaattgcc catcgtgtgg gtatgggggg cttcagtgct ctgcgtcagg tgcgtgaagg
     7201 ggcagaatat aaatacgtca ccaccggaga taaacaggcc actattgcac tggcgaccta
     7261 tggcgagctg ttcagtatca cccgtcaggc cattatcaat gatgatctga atatgctgac
     7321 cgatgtcccg atgaagctgg gccgtgcggc gaaatccact attgccgatc tggtttatgc
     7381 cattctgacg tctaacccga aaatctccac agataatgta agtctgttcg ataaagcgaa
     7441 acatgcaaac gtactggaga gcgcggcaat ggacgtggca tcgctggata aagcccgcca
     7501 gttgatgcgc gttcagaaag agggtgagcg tcatctgaat attcgtcctg cgttcgtact
     7561 ggtaccgacg gcgatggagt ctgttgctaa ccaggtcatt cgctcctcaa gtgtcaaggg
     7621 ggctgacatt aacgccggta ttattaaccc ggtgaaagat tttgcgaccg ttattgcaga
     7681 gcctcgtctt gatgataaca gccagaccac cttctacctg gctgcgtcca aaggctccga
     7741 tacgattgaa gtggcttatc tcaacggtgt ggatacgcca tatattgatc agatggaggg
     7801 cttcagtgtg gatggcgtga caacgaaagt gcgtattgac gctggtgttg cgccagttga
     7861 tcaccgcggc ctggtgaaat gtacggcgta aacgtcgcag acaacaactc tgatggcccg
     7921 taagggcttt ttttgtacct gaaatcagcc cctgaacggg gctgtgcgga gacagttatg
     7981 gcaaagaatt ttgtagaaga aggaaaaacg gtggcgattg ttgccagtgc agccatcagc
     8041 agcggagatc tggtgcaggt gggggatgtt tttgcggtgg cgctgaccga tattccacag
     8101 ggagaaacag gcgacggcat aaccgaaggt gtgtttatgc tgcctaagct gaaaacggat
     8161 gacatgaaaa cgggtaagaa ggtttatctg aagtcaggaa aagttcagct gactaacagc
     8221 ggctctgagc cgctggtcgg ggttgtctgg gcagatgccg gaaccagtgc agaagaagtg
     8281 ccggtaaaac tcaatgtctg atcccttttc ccggctggca gcgcgtatgg atgcgatcac
     8341 ggtcagaaag atgggaaaga cagcctcgat taatgatgtc gatatgattg tgatcccggg
     8401 agaaacactg gcagagctga atgctctgtc cggacctgcg gtctctctgg tggtgttttc
     8461 ttcgggatac cgcccacggc gcggggatcg cgttgtttat gacggacaac aatggacggt
     8521 cacacggcat gaacgtttta acggtaagcc aatgatcttt attgagtaaa gaggtgtggg
     8581 atgaaggggc ttgagaatgc catccgcaat ctgaacagcc ttgatacccg tatggtgcca
     8641 caggccagcg catgggcgat aaaccgtgtg gcacagaaag cggtctcggt tgccacccgg
     8701 caggttgccg ggaataccgt tgcgggagat aaccaggtga aagggatccc cctgaaactg
     8761 gtacgtcagc gtgtccgggt gtttaaagcc agtccgtcag gaaaaatgac ggtcaggatc
     8821 cgcgttaacc ggggcaatct gcccgccatt aagctgggga cagcccgggt cagactggcc
     8881 cggcgtggtg gaaaactgca gtaccgtggc agtgtgctga aggtgggtaa atatcttttc
     8941 cgggatgcgt ttattcagca actggcgaat ggtcgctggc atgtgatgcg acgtattgat
     9001 ggcaaaaatc gttaccccat tgatgtggtg aaaatcccgc tgtccggatc gctgacacag
     9061 gcatttgaag atgcccgcga ccacatcatt gctgcggaaa tgccgaaaca gctggggtat
     9121 gcactaaaac aacaactgag gttatggctg acccgatgaa ccgacataca caaatccgcc
     9181 aggccgtact ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc
     9241 ttccggcatt tattgatgcg caggaactgc ctgccgtggc ggtgtggctg agtgatgctc
     9301 agtacaccgg aaaaatgacg gatgaagatg actggcaggc tgttctgcat attgccgtct
     9361 tcatccgggc acaggcaccg gattcagagc tggatatgtg gatggagagc accattttcc
     9421 ctgccctgaa tgatataccg gcactttccg gactcatcga caccctgatc ccactcggtt
     9481 ttaactatca acgtgataat gagatggcca cctgggcgat ggcggaaatc acgtaccaga
     9541 tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc aaagacgaaa
     9601 ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc gaaccctttg
     9661 tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg cgaaatgacc
     9721 gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa aacgacaacc
     9781 caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc gggtgacagc
     9841 gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt tcgtatcaaa
     9901 tatcccaacg gcactgttga tgttttccgt ggctggctga gctcactggg taaaaccatt
     9961 gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg tccgtatctg
    10021 gcagaggaag gcactgaaac cgtgagcgtt accgggctga cagtatcacc ggcgtctgcc
    10081 agtgtaaaag tgggagcaac caccacgctg acctttacag taaaacctga cggagccagt
    10141 gacaaagcga tcagtgtgca ttcgtcagat ccacagactg ccacggttac cctgaacgga
    10201 cttgtggcca cggtaaaagg cgtgaagcag ggcagtgtca gcattgtggg catgaccgct
    10261 gacgggaatt ttgtggctgt gactgcggtg actgtcagcg cagcaggtta acaggacgat
    10321 actcatcatt tgccccggtt attcggggct tttttgcagg tggagaacat gatgtttctg
    10381 aaacaggaca cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct gtccgggctg
    10441 cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga tgccggggag
    10501 ggagaactgc cggaggctga acgacagatt gcttttctgc ggatggggat ggatatcaat
    10561 gcctggctgg tttcccgctc actgtggaat gcggatcagt ctaaggatgt agagacgctt
    10621 tgcgcatccg ttattacaac atggtcgtat gatgccctgg gtgcgggggc ggagatggtt
    10681 ctgtcgctga gcggtatggg ggccattgat aatgccgtgg atgatgagca tgaggcgctg
    10741 acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc cgggagttcc
    10801 ggcgggcaga ctggcggcgg atgctgtcgg aaatgtcggc cactgagctt ggtgagtggg
    10861 gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag tttgcctcgc
    10921 tgaaggcatt gatcgtgaga atggtgtccg gcagcagtga tgctgcggtg gctgatttca
    10981 gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg atgcatcttg
    11041 gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt cattgatttg
    11101 gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct tctgaatggt
    11161 gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga gcgtcagact
    11221 caggccgccc ggcagacaac gcaggcagct tcttcggctg caacagccgc atccgtccat
    11281 gcgcagacgg tggagaagaa cgcacaggct catgaacgca tggcccgcga ggtggagaaa
    11341 acccgccagc gcatggaggc actgagccag aaaatgcgcg aggaacaggc gcaggccatg
    11401 gctctggcgg aggctcagga taaagcggct gctgcgtttt atcgtcagat tgacagtgtg
    11461 aaacaggcca gtgcggggct gcaggaatta cagcgtattc agcagcagat ccgacaggcc
    11521 agaaacagtg gcgggattgg tcagcaggat tatctggcgc tgatttctga ggttactgcg
    11581 aaaacccgtg ttcttacaca ggctgaggaa gaggctaccc gacagaaagt ggcgtttatc
    11641 cgtcagctta aagagcaggc aacccgcctg aatctttcat cttctgagtt gcttcgtgct
    11701 aaggcagccc agctgggggt aagcagtgct gcagaagtgt atatccgcaa aatggagcag
    11761 gcaggaaaag ccacgcattc gctgggtctg aaaagtgcag cagcccgcca ggagataggc
    11821 gttctgatag gtgaactggc ccgcggcaat ttaggggcgc tgaggggatc cgggataacg
    11881 ctggctaacc gtgccggatg gatagacaca ctgatgtcac cgaaaggcat gatgcttggc
    11941 ggggttattg gcggtattgc cgcggccgtc tatggtctgg gtaaagcctg gtatgacggt
    12001 cagaaggagg gggaagaatt taaccgccag ttgtcgctga cggggcatta tgccggagtc
    12061 actgccgggc agctgtggac gctcagtcgt gctatttccg ggaatggtat cacgcaacat
    12121 gctgcagccg gtgcgctggc tcaggtggtg gggagtggtg catttcgtgg aaacgatatc
    12181 ggtatggtgg cgagagttgc cgcacagatg gagcgatcgg ttggccagtc ggtcagcgat
    12241 accataaatc agtttaagcg gctgaaggat gatcctgtaa atgccgcgaa ggctctggac
    12301 aatgagctgc attttcttac tgccactcag cttgagcaga tacgcgtcct tggggagcag
    12361 gggcggtcca gtgatgctgc acggatagcc atgtctgcac tggcagagga aaccggtcgg
    12421 cgtactgcgg atattgataa taacctcaat gcgcttggca gtacgctgaa gtatctgtct
    12481 gatttgtgga gtcgtttctg ggatgcggcc atgaatattg gtcgtgaaga ctcgctggat
    12541 gaacagattg ccgctttaca ggagaaagtg tcgcgggcga aaagactccc ctggacggca
    12601 tcatcttctc aggttgaata cgatcagcag cgtcttaacg atcttcagga gaaaaaacgc
    12661 cagaaggatt tgcaggatgc aaaagagcag gcagagcgga attatcagga gcaacagaaa
    12721 cgccgtaatg ctgaaaatgc tgcactgaac cggatgaatg aaacggaagc agcacgacat
    12781 cagcgtgaaa ttgcgcgtat taatgccatg cagtacgccg atcaggctgt cagggatgcg
    12841 gcgatacaac gtgaaaatga acgttacgag aaagccctgg catccggtaa gaaaaaaaca
    12901 cgcgaaaccc gtaatgatga ggccacccgg ttattgctgc agtacagtca gcaacaggca
    12961 caggtggaag gacagattgc tgctgccaga cagtcagcag gcattgccac ggaaaggatg
    13021 acagaagcgc ataaacagct tctggctctg cagcagcgca tcagcgacct ggacgggaaa
    13081 aaactgacgg cagatgaaaa gagtgtgctg gcccgtaaag atgaactgat tcaggcactg
    13141 acgctgctgg atgtaaaaca gcaggagctt cagaaacaga cggcactcaa cgagctgaag
    13201 aaaaaaacaa ttcagctgac cagtcaactg gctgaagaag agcgcgctca gcgtcagcaa
    13261 catgacctgg atatcgccac ggtgggtatg ggtgatcagc agcgacagcg atatcaggta
    13321 caactgagtc ttcgccagaa ataccagcaa cagctggagc agttgaggcg ggatagtgag
    13381 cagaaaggga catataacac ggatgactac agaaaggccg agcaggcgct gacggagagc
    13441 ctgaaccgac aactgaatga gaatcgccgt tactggcaac agcttgaagt tgtgcagggt
    13501 aactggaaaa acggagtcct gcgtgcattt caggatttta ccgtggatgc agataatacg
    13561 gcagaaacag cagaacaggt gttctcgtca gccttcagca acatgggaaa tggcctggca
    13621 acttttgtca ctaccggcaa actcaatttc aaatccttca cctcttctgt gctgtcagat
    13681 atggcgaaaa tcctggcgca ggcaaccatg atgaaatcga taaaagggat tggcagtgta
    13741 ctgggatttg atctcagcag cctttccctg aatgccaatg gggggattta tcagtctgct
    13801 gatttgagtc gttacagtgg cacggtggtt aaccgtccga cgttttttgc ttttgcaaaa
    13861 ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccattgcg tcgtggtgct
    13921 gacggtaagc tgggggttgt ggcggatatt ggggggtcag gtatggcgat gttttccccg
    13981 cagtacaaca tcgagatcaa taacgatggc acgaacgggc agataggtcc ggctgccctg
    14041 aaggcggttt atgacctcgg gaaaaaagcg gcagcggact ttatgcaaca gcaggcccgt
    14101 gatggtggtc ggttaagtgg agcatatcgg taatggagac gtttcactgg aaagtgcgcc
    14161 cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc gatggttatg
    14221 aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg acgatacgtg
    14281 ttcgtaaatg tgaccaccca tctttaaaag cctttctgga acggcacggt ggcgtccgtg
    14341 catttcagtg gacgccacct tatgactgga aaccgatcag ggtggtttgt cgtaaatggt
    14401 cggcaagcgt gggggcgctg tgggtaacca taacggcaga ttttgaacag gtcgtggcat
    14461 aggaggctct gatgcaggat attccacagg aaacacatca tgagacgaca cgcctcactc
    14521 agtcagccca ggtggtgctc tgggaaatcg atctgacaga ggtcggtggt gaacgttatt
    14581 ttttctgtaa tgagcagaac gaaaaaggtg agccggttat ctggcagggg cggcagtatc
    14641 aggtataccc cattcagggg acgggatttg aactgaacgg caagggcagt gctgcccgtc
    14701 cgacactgac ggtttctaac ctgtacggta tggtcaccgg gatggcggaa gatctgcaga
    14761 gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga
    14821 acttcgtcaa cggaaacagt gacgccgatc cggagcagga ggtgatcagc cgctggcgca
    14881 ttgagcagtg cagcgaactg agcgcggtga gtgcctcttt tgtactgtcc acgccgacgg
    14941 aaacggacgg cgctgttttt ccgggacgta tcatgctggc caacacctgc acctggacct
    15001 atcgcggtga tgagtgcggt tatcacggtc cggcagtcgc ggatgaatat gaccagccga
    15061 cgtccgatat cacgaaggat aaatgcagca aatgcctgag cggctgtaag tttcgcaata
    15121 acgtcggcaa ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa tcccatgaca
    15181 gagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgcggc
    15241 ttcgtggtga gagcgccgga gggggaaaga tattttccct gcgtgaatat ttccggtgag
    15301 ccggaggatt atttccggat ggctccggag gactggctgc aggcagagat gcagggtgag
    15361 attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg
    15421 cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag
    15481 ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac
    15541 acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg
    15601 gatgactggt ggcgtaacgg tcagaatctc tatctggata atctggaggc cacagggctg
    15661 tatcaggtgg cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca
    15721 tcggtgccga atcatgccgc catttactgc ggcgacggtg agctgctgca ccatattcct
    15781 gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc
    15841 tggcgtcacc gggcatggca cgcatctgcc tttacgggga tttacaacga tttggccgcc
    15901 gcatcgacct tcgagtgaaa acggggtctg aagccatccg ggcgctggcc atgcagatcc
    15961 cggcgtttcg tcagaaactg agcgacggct ggtaccaggt acgcattgcc gggcgtgatg
    16021 caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt gccgtgatcc
    16081 acatcgtgcc gcgtctggcg ggagctaaaa gtggcggtgt gtttcaggtg gtgctggggg
    16141 cggcgctgat tgcggtggca tggtggaacc ctgtgggctg gctgggtgcc gcggctgtat
    16201 cgggcatgta tgcggcaggg gccagtatga tcctgggcgg agtggcgcag atgctggcac
    16261 cgaaagccag gacgcccacg gcagcaagta cagataacgg caaacagaac acctatttct
    16321 cctcactgga taacatggtt gcccagggca atgttctgcc cgttctgtac ggtgaaatgc
    16381 gcgtggggtc gcgggtggtc tctcaggaga tcagcacggc agacgaaggg gatggtggtc
    16441 aggttgtagt gattggtcgc tgatgaaaaa cgttttatgt gaaaccgcct ccgggcggtt
    16501 ttgtcgttta tggagcgtta ggaatgggta aaggcagcag taaggggcat actccgcgcg
    16561 aagcgaagga caacctgaag tccacgcagt tgctgagtgt gatcgatgcc atcagcgaag
    16621 ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt acgccagtgc
    16681 tggacactga ggggaatacc aacatctccg gcgtcacggt ggtgttccgg gccggtgagc
    16741 aggagcagac accgccggag ggatttgaat cctccggatc cgagacggtg ctggggacgg
    16801 aagtgaaata cgacacgccg atcacccgga ccatcacgtc ggcaaacatc gaccgtctgc
    16861 gctttacctt cggtgtgcag gcactggtgg aaaccacctc aaagggggac aggaatccat
    16921 cggaagtccg cctgctggtt cagatacaac gtaacggtgg ctgggtgacg gaaaaagaca
    16981 tcaccattaa aggcaaaacc acctcgcagt acttggcctc tgtggtggtg gataacctgc
    17041 cgccgcgccc gttcagtatc cggatgcgca ggatgacgcc ggacagcacc acagaccagc
    17101 tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc
    17161 cgaacacggc actggtcggc gtacaggtgg attcggagca gttcggcagc cagcaggtga
    17221 gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc gtcgaattat aacccgcaga
    17281 cgcgacaata cagcggtatc tgggacggaa cgttaaaacc ggcatacagc aacaacatgg
    17341 cctggtgtct gtgggatatg cttacccatc cgcgctacgg catggggaaa cgtcttggtg
    17401 cggcggatgt ggataaatgg gcgctgtatg tcatcggcca gaattgcgac cagtcagtgc
    17461 cggacggctt tggcggcacg gagccgcgca tcacctgtaa tgcgtacctg accacacagc
    17521 gcaaggcgtg ggacgttctc agtgatttct gctcggcgat gcgctgtatg ccggtatgga
    17581 acgggcagac gctgacgttc gtgcagaacc gaccgtcgga taaggcgtgg acctataacc
    17641 gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc agcgccctga
    17701 a
//
LOCUS       DAZFDP010000099        17676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFDP010000099 DAZFDP010000000
VERSION     DAZFDP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..1231
                     /gene="arnB"
                     /locus_tag="ACHJ1G_003357"
     CDS             92..1231
                     /gene="arnB"
                     /locus_tag="ACHJ1G_003357"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4765154.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1235..2203
                     /gene="arnC"
                     /locus_tag="ACHJ1G_003358"
     CDS             1235..2203
                     /gene="arnC"
                     /locus_tag="ACHJ1G_003358"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4765155.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2203..4185
                     /gene="arnA"
                     /locus_tag="ACHJ1G_003359"
     CDS             2203..4185
                     /gene="arnA"
                     /locus_tag="ACHJ1G_003359"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4765156.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4182..5072
                     /gene="arnD"
                     /locus_tag="ACHJ1G_003360"
     CDS             4182..5072
                     /gene="arnD"
                     /locus_tag="ACHJ1G_003360"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4765157.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5072..6724
                     /gene="arnT"
                     /locus_tag="ACHJ1G_003361"
     CDS             5072..6724
                     /gene="arnT"
                     /locus_tag="ACHJ1G_003361"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4765158.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6721..7056
                     /gene="arnE"
                     /locus_tag="ACHJ1G_003362"
     CDS             6721..7056
                     /gene="arnE"
                     /locus_tag="ACHJ1G_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4765159.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7056..7442
                     /gene="arnF"
                     /locus_tag="ACHJ1G_003363"
     CDS             7056..7442
                     /gene="arnF"
                     /locus_tag="ACHJ1G_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4765160.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7436..7702)
                     /gene="pmrD"
                     /locus_tag="ACHJ1G_003364"
     CDS             complement(7436..7702)
                     /gene="pmrD"
                     /locus_tag="ACHJ1G_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4765161.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7812..9167)
                     /gene="menE"
                     /locus_tag="ACHJ1G_003365"
     CDS             complement(7812..9167)
                     /gene="menE"
                     /locus_tag="ACHJ1G_003365"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4765162.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9164..10126)
                     /gene="menC"
                     /locus_tag="ACHJ1G_003366"
     CDS             complement(9164..10126)
                     /gene="menC"
                     /locus_tag="ACHJ1G_003366"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4765163.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10126..10983)
                     /gene="menB"
                     /locus_tag="ACHJ1G_003367"
     CDS             complement(10126..10983)
                     /gene="menB"
                     /locus_tag="ACHJ1G_003367"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4765164.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10998..11756)
                     /gene="menH"
                     /locus_tag="ACHJ1G_003368"
     CDS             complement(10998..11756)
                     /gene="menH"
                     /locus_tag="ACHJ1G_003368"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4765165.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11753..13423)
                     /gene="menD"
                     /locus_tag="ACHJ1G_003369"
     CDS             complement(11753..13423)
                     /gene="menD"
                     /locus_tag="ACHJ1G_003369"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4765166.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13512..14807)
                     /gene="menF"
                     /locus_tag="ACHJ1G_003370"
     CDS             complement(13512..14807)
                     /gene="menF"
                     /locus_tag="ACHJ1G_003370"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4765167.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14886..15191)
                     /gene="elaB"
                     /locus_tag="ACHJ1G_003371"
     CDS             complement(14886..15191)
                     /gene="elaB"
                     /locus_tag="ACHJ1G_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4765168.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15246..15707)
                     /locus_tag="ACHJ1G_003372"
     CDS             complement(15246..15707)
                     /locus_tag="ACHJ1G_003372"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4765169.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15772..16689
                     /gene="rbn"
                     /locus_tag="ACHJ1G_003373"
     CDS             15772..16689
                     /gene="rbn"
                     /locus_tag="ACHJ1G_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4765170.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16877..>17676
                     /locus_tag="ACHJ1G_003374"
     CDS             16877..>17676
                     /locus_tag="ACHJ1G_003374"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO4765171.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGH"
BASE COUNT         4183 a         4752 c         4443 g         4298 t
ORIGIN      
        1 ccttaaaatt tcgctccaaa tggcaaaata tacacaacac tctttatagc aaatataagt
       61 ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc
      121 agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac
      181 aaccggtccg aagaatcagg cgcttgagca agccttttgc cagttgacgg gaaatcagca
      241 tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat
      301 tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat
      361 ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac cgcgatacgc cgatggtcac
      421 gcctgaagcg atcgagtcag ccataacgcc acgcactaaa gccatcattc cggtgcatta
      481 tgccggtgcg ccagcagata ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc
      541 agttatcgaa gatgctgccc atgccgtcgg tacgtattac aaagggcgac atattggcgc
      601 aaaaggtacc actatttttt catttcatgc cattaaaaat attacctgtg cggaaggtgg
      661 cctgattgta actgataatg aaaaccttgc ccgccagcta cggatgctga aatttcacgg
      721 tctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt
      781 aacaccgggc tataagtaca atctgaccga tattaacgcc gcaattgccc tgacacagtt
      841 agcaaaatta gagcacctca acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc
      901 actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg
      961 gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat
     1021 ggaagcgtta aaagaaagag gcattggtac cgggttacat ttccgcgccg ctcacacaca
     1081 aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg aataccgaat ggaatagcga
     1141 acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg accgtgtcat
     1201 cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa
     1261 agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg
     1321 caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg
     1381 cagtagcgat aattccgcgc atatgctggt cgaagcctca caagcggaga acagccatat
     1441 tgtgtctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg cgggattcag
     1501 tcacgttacc ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga
     1561 aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca
     1621 gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaacc ggcttattca
     1681 gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca
     1741 tattgtcgat gcgatgttgc actgccatga acgtagcacc tttatcccga ttctggcgaa
     1801 tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat tccgagcgtg agtttggtga
     1861 atccaaatac agttttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac
     1921 cacaacgccg ctacgtatgc tgagtctgct cggcagcatt attgcgattg gaggttttag
     1981 cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg
     2041 cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg
     2101 attactcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt
     2161 tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt
     2221 gcctaccacg atatgggatg cctcggtatt gaagctctgc tggctgccgg ttacgaaatt
     2281 agcgccattt ttacccatac cgataatccc ggtgaaaaag ccttttatgg ttcggtggct
     2341 cgtctggcag cggaaagagg cattccggtt tatgcgccgg atgacgttaa tcatccgctg
     2401 tgggtggaac gcattgccca actgtcgcca gatgtgattt tctcttttta ttatcgccat
     2461 cttatttgcg acgaaatttt gcagctcgct cccgcaggtg catttaatct gcatggttcg
     2521 ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtcaa cggtgaaacg
     2581 gaaactggcg ttacattgca ccgaatggtg aaacgtgccg atgccggggc cattgtggcg
     2641 caactgcgca ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
     2701 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
     2761 gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc gcagaacgcc ggatgacagt
     2821 ttccttgaat ggcataaacc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
     2881 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
     2941 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
     3001 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aggcgggcga cggcattact
     3061 atgcagggct cgcaattagc gcagacgctg ggcctggtgc aaggttcacg cttgaatagc
     3121 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
     3181 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
     3241 gatattggca gtgatgcgat aagccgtttt ctgaatcatc cgcattttca ctttgttgaa
     3301 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
     3361 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
     3421 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgtgaagta ccgtaagcga
     3481 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
     3541 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtatca
     3601 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
     3661 ctcttccgcc cgtttaactg gatgggaccg cgactggata accttaatgc ggcacgaatc
     3721 ggcagctccc gcgctattac gcaactcatt ctcaatctgg tagaaggttc accgattaag
     3781 ctgattgatg gcggaaaaca aaaacgctgc tttactgata ttcgcgatgg tatcgaggcg
     3841 ttataccgca ttatcgaaaa tgcgggaaat cgctgcgacg gtgaaattat caacattggc
     3901 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
     3961 aaacatccgc tgcgccatca tttcccaccg tttgcgggct ttcgcgttgt cgaaagtagc
     4021 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
     4081 caccgctgcc tggactggga gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg
     4141 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtaggcttac
     4201 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct
     4261 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
     4321 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaatgcgg
     4381 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
     4441 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg
     4501 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
     4561 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
     4621 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
     4681 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccattgctc gaatcaggaa
     4741 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
     4801 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
     4861 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
     4921 atctcctcaa acgcgcagct caggaaggag tgacattttg ccctttaagc gaactgttat
     4981 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
     5041 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct
     5101 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
     5161 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
     5221 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
     5281 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
     5341 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
     5401 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
     5461 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
     5521 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
     5581 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
     5641 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
     5701 ggcggttatc agttgcgtac tgacggttct cccttgggga ctggcgatag cgcagcggga
     5761 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactggatga
     5821 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
     5881 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
     5941 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
     6001 taaattgccc acctatattc tttcctgctt tgcatctctg gcaatgctga tggcgcatta
     6061 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
     6121 cgcttttggc gtcactggca ttattgccac atttgtggtc tccccgtggg gaccaatgaa
     6181 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
     6241 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggccttttgc
     6301 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagttat
     6361 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
     6421 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
     6481 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
     6541 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
     6601 ggggattatt actctcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
     6661 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
     6721 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
     6781 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
     6841 ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt cttgcagaac
     6901 gtaccggtag gcattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
     6961 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
     7021 attggtggca ttgtgatcct cgggagtacg gtgtaatggg cctgatatgg ggattattca
     7081 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcaagt catctgccgc
     7141 cgatgacgca cttatgggat tttatcgcgg ctctgttagc ttttggcctg gatgcccgga
     7201 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacgctac
     7261 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
     7321 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
     7381 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
     7441 gagttttccc tgccgctgta cactgccgtt cccactcatc ggggctataa ctgcttgccg
     7501 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
     7561 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
     7621 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
     7681 attttttgac cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
     7741 acagattaac agataaactc tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa
     7801 cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta
     7861 ataccgccgt ttttcagctc cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga
     7921 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
     7981 gctaccggcc gatgaccaaa ctccttgtcg gcaacgggaa cgataaacac ctgcaaaacc
     8041 gcaggatgtg cagcaataac acgctcgact tcttccggct gaataccctc tccgccactg
     8101 aagaatagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
     8161 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
     8221 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
     8281 cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg
     8341 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
     8401 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
     8461 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcatg agtacagcct
     8521 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
     8581 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
     8641 tcgtgatcgc caaacggaat caacgataac accccttcgg cactggcaag atgggcctga
     8701 taagtatgga cagcggcttt cggcaacccg gtagaacccg aggtcaacgt cattgagcac
     8761 agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa cccgctgaat gtgcagcgac
     8821 gttaacgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
     8881 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
     8941 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
     9001 atcacgccgc tgccctcaac aacgccctgc gccgcaaagc ctgaggctaa ttcatcgacg
     9061 cgagcacaaa gctcgcgcca gttaagttgc tcgtcattaa gacgtaaggc gatggcttct
     9121 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aaccgctcca
     9181 gtgcatcaac ttccacgaca ggcagcgtgc tacccggcca gcgacgtacc tgctgcgcct
     9241 gcatcagatc cagcgtgtcc agccctggaa tggtgtccgg cgttaaccag gcggcaatcc
     9301 gcgccagttg cgttaagcct aagctcgatt caatggaaga actgatcacc gccgtcagcc
     9361 ccagcgcgtg cgccgcctgt acctgctcgc gtactttctc cagactgccc gtgagcgtgg
     9421 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
     9481 agctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
     9541 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
     9601 tggcaaactg ctggcctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
     9661 gatccggaat tgcctccagc aacagattaa ccaccatgcc gtcgcgcacg gcttcgtaca
     9721 atcccacttt gaccttcgcc actttctcgc ctggcatatc agcaagtttg aggatcagat
     9781 cgtcaggatc gccattacac agtggtgccg cacggtaatt ggccgcctgc gggagcgtat
     9841 ctgccagctc cgccaatgca cagcttacgc caaaggcaac ggaaggcatc tgcggtagct
     9901 cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc
     9961 aggtttcctg gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct
    10021 cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc
    10081 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    10141 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    10201 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc
    10261 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    10321 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    10381 gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc
    10441 tcgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa
    10501 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc
    10561 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    10621 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaagt gatgtacgcc
    10681 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    10741 cgctttatcg cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc
    10801 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    10861 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcgaa
    10921 accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat caggataaat
    10981 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg
    11041 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    11101 tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    11161 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    11221 atggcggcaa gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga
    11281 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    11341 ggttctgtgc aaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt
    11401 tcagcatttt gcagccccgg atgcccgcct tcgacaataa ccccacaaag ccccgccagc
    11461 ccctggcaag ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag
    11521 tcaaggatgt tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca
    11581 tcgacgctaa tcgccgccga accaccatga cctgggagat caacatacaa ccgtgagtag
    11641 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    11701 aacaccagcc agggtaaacc tggttttccg tgttttgcct gcgcgtgcag gatcataaat
    11761 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    11821 tttcaatcac cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct
    11881 gccagttttg cggacgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    11941 cgttttgcgg catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga
    12001 aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca
    12061 ataacgccag cgcgttgaga tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    12121 gtttgccgct tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg
    12181 cccctcggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    12241 gtaccaccag gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    12301 gatgcgccag ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct
    12361 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    12421 ccagccagtc ggcaatattg gcaattaagc gacgtccgcg atggtgtgcc ggatcaagtc
    12481 gcccttcaat gtcatcaaca atccagtact cttcgggttc acagcttgcc tgccattgca
    12541 ggagccgttt ccccgtcagg ctgcttccca gttgcaccac aatttgcgcc tgctgcagct
    12601 cgctggtcgc tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt
    12661 gtgacagcac atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt
    12721 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga
    12781 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    12841 cctgccacca gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat
    12901 acagcggttc agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag
    12961 cgtggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    13021 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc
    13081 cgcagtcaat tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta
    13141 acccggcttc aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca
    13201 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc agtccacgct
    13261 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    13321 tagaacgcga gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    13381 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    13441 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    13501 gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    13561 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atatagccgc
    13621 acgacattgc cgctgatttt tgctgaacgc agggaaacgc agaattcgct ttgttgtaat
    13681 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    13741 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    13801 aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    13861 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    13921 aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc
    13981 atcagccact ctcctaactg ctgcgcctgc ttatcatcag gattatttgc tactgttccc
    14041 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    14101 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    14161 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    14221 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    14281 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    14341 aacccaggca agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca
    14401 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    14461 cgtcgccatt ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc
    14521 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    14581 aacgacgtaa aacgggtaat cgcgcccagg acggctgctt cttcatcacc attacgttgt
    14641 tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    14701 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    14761 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    14821 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    14881 aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc gcccacacca
    14941 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaactgc ctgcttcgcc
    15001 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    15061 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    15121 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    15181 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagggtt
    15241 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    15301 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    15361 gccccgaggt aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc
    15421 atcagctgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac ccgacctata
    15481 acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac cagttcatca
    15541 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    15601 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    15661 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    15721 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    15781 atttttttag gtacttcggc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    15841 cttaacctgc aacatccgac ccagagcggt ctttggttgt ttgactgcgg tgaaggcacc
    15901 cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt
    15961 caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca
    16021 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    16081 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    16141 ggcgaaattc tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg
    16201 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    16261 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    16321 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    16381 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    16441 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    16501 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc
    16561 ggtaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcagcacctg
    16621 ttacgtgaat gcaggtcaat tttcccggcg actgaactag cgaatgattt caccgtgttt
    16681 aacgtttaac tctgcttatt attaaaaaca gggcgaaact tgccctgtta tcgcaacccg
    16741 cgctacatat atccacccac acgggcatat tgagcgattt ttgatattta ttcatctcct
    16801 tacgcactat attcagagca ataagttgcc tctttttatc ttcattcagc acaacctttg
    16861 caactaaaag gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac
    16921 ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt
    16981 aaaaaaaaca gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt
    17041 aggttatgat gaatttggaa aaaaagctga agatattctt taccatattg ttagaaaccc
    17101 aacaaatgag accctatcga ttatccgact tattaaaaat gcctgtttaa aattatataa
    17161 tttggcacat accgcaacca actcccacct taaaccaact ggtccggata attcagatgt
    17221 tctcctgttc aaaaagctat tctccccttc gaaattaatg acaattatcg gtgatgaaat
    17281 tcctcttata tctgaaaaac agtcgctttc aaaggtgctt ttaaatgatg agaataatga
    17341 actgagtgat gatacaaact tctgggataa aaatcgtcaa ttaaccacag atgaaatagc
    17401 ttgctatctt cagaagatcg ccgctaatgc aaaaaatact caagtcaatt atcctactgg
    17461 tctctacgtc ccctactcca ccagaactca cctggaagac gctctcaatg aaaatattaa
    17521 gagcgatcca tcatggccga atgaagtcca gttattccac ataaatactg gcggacactg
    17581 gatattagtt tcgctacaga aaatagtaaa taaaaaaaat aataaactac aaataaaatg
    17641 cgtcatattc aactcattgc gtgcactagg ccatga
//
LOCUS       DAZFDP010000100        17666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFDP010000100 DAZFDP010000000
VERSION     DAZFDP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..570
                     /locus_tag="ACHJ1G_003375"
     CDS             67..570
                     /locus_tag="ACHJ1G_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4765172.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(637..2094)
                     /gene="nuoN"
                     /locus_tag="ACHJ1G_003376"
     CDS             complement(637..2094)
                     /gene="nuoN"
                     /locus_tag="ACHJ1G_003376"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4765173.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2101..3630)
                     /gene="nuoM"
                     /locus_tag="ACHJ1G_003377"
     CDS             complement(2101..3630)
                     /gene="nuoM"
                     /locus_tag="ACHJ1G_003377"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4765174.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3794..5635)
                     /gene="nuoL"
                     /locus_tag="ACHJ1G_003378"
     CDS             complement(3794..5635)
                     /gene="nuoL"
                     /locus_tag="ACHJ1G_003378"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4765175.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5632..5934)
                     /gene="nuoK"
                     /locus_tag="ACHJ1G_003379"
     CDS             complement(5632..5934)
                     /gene="nuoK"
                     /locus_tag="ACHJ1G_003379"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4765176.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5931..6485)
                     /gene="nuoJ"
                     /locus_tag="ACHJ1G_003380"
     CDS             complement(5931..6485)
                     /gene="nuoJ"
                     /locus_tag="ACHJ1G_003380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4765177.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6497..7039)
                     /gene="nuoI"
                     /locus_tag="ACHJ1G_003381"
     CDS             complement(6497..7039)
                     /gene="nuoI"
                     /locus_tag="ACHJ1G_003381"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4765178.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7054..8031)
                     /gene="nuoH"
                     /locus_tag="ACHJ1G_003382"
     CDS             complement(7054..8031)
                     /gene="nuoH"
                     /locus_tag="ACHJ1G_003382"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4765179.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8028..10754)
                     /gene="nuoG"
                     /locus_tag="ACHJ1G_003383"
     CDS             complement(8028..10754)
                     /gene="nuoG"
                     /locus_tag="ACHJ1G_003383"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4765180.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10807..12144)
                     /gene="nuoF"
                     /locus_tag="ACHJ1G_003384"
     CDS             complement(10807..12144)
                     /gene="nuoF"
                     /locus_tag="ACHJ1G_003384"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4765181.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12141..12641)
                     /gene="nuoE"
                     /locus_tag="ACHJ1G_003385"
     CDS             complement(12141..12641)
                     /gene="nuoE"
                     /locus_tag="ACHJ1G_003385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4765182.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12644..14446)
                     /gene="nuoC"
                     /locus_tag="ACHJ1G_003386"
     CDS             complement(12644..14446)
                     /gene="nuoC"
                     /locus_tag="ACHJ1G_003386"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4765183.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14540..15202)
                     /gene="nuoB"
                     /locus_tag="ACHJ1G_003387"
     CDS             complement(14540..15202)
                     /gene="nuoB"
                     /locus_tag="ACHJ1G_003387"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4765184.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15218..15661)
                     /gene="nuoA"
                     /locus_tag="ACHJ1G_003388"
     CDS             complement(15218..15661)
                     /gene="nuoA"
                     /locus_tag="ACHJ1G_003388"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4765185.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16292..17230)
                     /gene="lrhA"
                     /locus_tag="ACHJ1G_003389"
     CDS             complement(16292..17230)
                     /gene="lrhA"
                     /locus_tag="ACHJ1G_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4765186.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4395 a         4943 c         4584 g         3744 t
ORIGIN      
        1 tgcacatttt attttccctc gtctgtttta agatgcgaaa catgaaattc aatggaggaa
       61 aaataaatgg gaatgattgg ttattttgca gaaattgact cagaaaagat taaccaactg
      121 cttgagtcta ctgaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc
      181 cgactggata ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct
      241 tttgatcccg cgaagaatga tccgctgagt cgtgccgtgc ttggtgaaca cagcctcgaa
      301 gatggcatag atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac
      361 cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat
      421 gaaatggaga tttatccggg agtgaccttt tcagaagagt tagaggggca actctttgct
      481 tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg
      541 aatcatgcgc tgacggttat tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga
      601 acatgtcggc ggctttctga cttacaaact aacagattac atcagcggca ttgctaaccg
      661 cacaatgcta atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagaaat
      721 cagcaccacg ataccgcccg cgctgtactg ccagtttgat ggtgcatcgc gacccggttg
      781 ttccggggcg tgaagataca ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc
      841 cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag
      901 cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag acagcatcat
      961 caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag
     1021 ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc
     1081 gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg acatctcacc
     1141 agtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc
     1201 gagcagacgt ttgatgttgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc
     1261 aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg gtgcgtagag
     1321 gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga
     1381 aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagcga
     1441 gagtttgaag ccgaggccaa caatcatcag tccgaaacct gccagcaaca gcggctcgtt
     1501 gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgtgc
     1561 atacaccagc gccataccaa acagcaggaa agaagacgct gcggcagaaa ggatggtgta
     1621 tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag
     1681 cggcaaagag atcagttcaa taccgaggaa caaagacgcc agatggttgg cattcgccag
     1741 caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct tgttgtcgtt
     1801 atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag
     1861 cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc
     1921 cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt
     1981 agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa
     2041 gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat
     2101 ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga
     2161 gcgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag
     2221 caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg
     2281 gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa
     2341 gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag
     2401 aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa
     2461 gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat
     2521 gtcgcgggta tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga
     2581 caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta
     2641 gatagcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat
     2701 atcggtctgg gcgaaggcca tccacgcacc gtagaagatg ccgataacac ccagccacat
     2761 agcaattggc gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga aacgcagcaa
     2821 accgtaagcg gcagttttca gcaagatccc cgcgaggtca acggaaccgg cggtcggagc
     2881 ctgggagtgc gcatccggca gccagccatg cagcggaacc accggcattt tgactgcgaa
     2941 ggcgatgaag aagcccagca ttaacaggta ttccacacca ctggacattg gcgtattcag
     3001 cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc
     3061 caggatggca atcaacatca ccaggccact cgcctgggtg taaatgaaga acttggttgc
     3121 cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta
     3181 catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa
     3241 cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt
     3301 ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg tcagcacgac
     3361 catcagcagc gacagcccgt caatggcgag atgaatagag ataccaaaac gcgggatcca
     3421 cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc
     3481 gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat
     3541 ccagcgtggc accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccaataaa
     3601 gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt
     3661 caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg
     3721 tcggatgcac cgcgaaggcg gcttatccga cctacgttct tatatgccca ggggcaatcc
     3781 cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc
     3841 tcatggatgc cacataccag cgcagatagc cgttctcact taacagcaga cctttacctg
     3901 caaagcggga aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca
     3961 accaggcaat acccaggaac ggcttgacga acactttgtc atacagccag tcaaatcccc
     4021 aggcgttgta ccaccaggtg cccagcagac ggcccggcgc actgttggcg atggaggtca
     4081 ccagagtacg tttacccagc cacagccagg ctgccagcag aatgccgacc accgcgacca
     4141 cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa
     4201 gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca
     4261 caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc
     4321 cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca
     4381 gacctgccac catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct
     4441 tactgaagaa gcccgcagtg accagcggta gtgccgacag tgctgcgccg cccaccagga
     4501 agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt
     4561 cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg
     4621 cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca
     4681 tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca
     4741 gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc agatgcagaa
     4801 cttccggcgt caccaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta
     4861 ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa
     4921 gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg
     4981 tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt
     5041 cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa
     5101 cgtcacccac acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg
     5161 tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca
     5221 tcagcagcag gttatcggcg agaaccagaa ctaccatgct ggcgatgaac aggttggtgt
     5281 aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa
     5341 taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg tccagcacca
     5401 ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg
     5461 actgctcgcc gttagcgaag aaatcaacgc cgataaaggc ggttaccagc gccgccaggc
     5521 ccacagagcc aacgccgacg atcgccgaaa cgttttcaga ccagcgccca cgggagaacg
     5581 ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg
     5641 catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag
     5701 cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt acatcacctg
     5761 accgtcggtc tgaccccagt agcttccggc gaccacaaag gccagcgcgg aggcgttaat
     5821 catgatttcc agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa
     5881 gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc
     5941 tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc
     6001 tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt
     6061 tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc
     6121 gtaccgtcga taccctgatc attaacacca aggatggcgt aaacaatcac caccagcatg
     6181 atggccgaca aaattgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt
     6241 tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag caccataatg
     6301 gcacccgcgt agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac
     6361 accccggaaa tcgccagcag cgaaataatc aggtacagca gtgcgtgtac cggattggta
     6421 tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac
     6481 tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct
     6541 ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt
     6601 ccgggtattt gcccggaccg gagatcagca gatcctcttt ttcgtaaacc agatcctggc
     6661 gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt
     6721 cttcgcacag accacagaaa atgcagcgtg agaagttgat gcggaaaaat tccgggtacc
     6781 agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg
     6841 cgcagaggtt acaggctacg caacgctctt cgccgtccgg gtcgcgggtc agaacgatac
     6901 gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt
     6961 tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta
     7021 acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc
     7081 cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat
     7141 tacctggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa
     7201 gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc
     7261 gaagaacagc gtcaccatca atgcagagat ggtcacaatc ccgatgtact cacccacgaa
     7321 gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc
     7381 ggcttccggc tggtcaaacg ggtgacggtg acataccgcc acgcccgcga tggcaaaggt
     7441 aataaaacca aagaattgcg ggataacgtt ccacacatgc gcctggctgt tgacgatgtc
     7501 ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac
     7561 ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcaacgagt atttgttgtt
     7621 acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa
     7681 caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca caatcgcaaa
     7741 ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga
     7801 gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag
     7861 cgaaccgccc cagccaacac ggttaggtcc gtaacggttc tggaacagac ccagcaggcg
     7921 acgttcgcca aagctcatga atgccccgca ggtgacaacc accagcagga tcaccaccgc
     7981 tttgaggatg gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc
     8041 tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga catacccatc
     8101 ggcaaaccca cctgccctgc cgtcagccct tcagcgattt caaccggcag cgtgaccgtg
     8161 ttgccatcgt aactaaagga gacgcgagcg cctgcgttca cgcccaactt cgcggcatcc
     8221 gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt
     8281 gacaattcat cgctgccaaa caggtggtaa tacggcgcga tacgccattt cccgtcctgc
     8341 ggctggaagc gtgccggtac gctggtgaag taatccagac cattttcgct ggtttcaaac
     8401 agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc
     8461 cacgcctgcg gggagttcca gcccggcgcc caggcaaacg gcacttgcga acggtgcgca
     8521 gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga
     8581 cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctgta acggtgcggt
     8641 tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata
     8701 cctgccagtt ccgggatttt cgccacaaca gcgtcaatca catggtcgag ctgcgtccag
     8761 tccacttcac ggctcagcag ggtgctgtgc agcgagtgta accagcgcca gctttccagc
     8821 atgacagttt tgctgtcgta ataagcagga tcgtaaacct ggaagaaacg ttgggcgcgg
     8881 ccttcgttgt tgatcaccgt accgtcgctt tcagcaaagc tggcagcaga aagtaccaga
     8941 tgggcgtttt ccataatcgc tgtgcgttga tgatcaacca ccatcaccag cggcgcttta
     9001 gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc
     9061 accaccgcgt cggcgcgtcc ggtttccagt tcggtcaacg cttcttcaag cgaaccgcca
     9121 cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat accaacgtca
     9181 gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag cctgaatcac ctctatgcta
     9241 ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg
     9301 acgtcgattt tgctttgcag ctcaggttcg ataccgtcaa ccgctggcgc agagttatcc
     9361 agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc
     9421 cacgccgcga tatcatccag acgggtgtca tcaacgttgg taacaaacag cggatgcttc
     9481 gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc
     9541 gccatttcgc gcgctttacc tttcacagcc tgacgcactg ccagcgcgac gcgcgcgccg
     9601 gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcaga
     9661 gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc
     9721 tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagttcacg cagcgcaaag
     9781 ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga ctgacgcaga
     9841 atatctgccg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga
     9901 cgctgtactg gctgacgcgg acgatccttc aggttgacgt aaccgtaacc gaaacgacca
     9961 cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt
    10021 tcgccgtaac gttcaccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc
    10081 ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtaaat
    10141 acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct
    10201 tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa cgcccagatc tgtaccgtca
    10261 gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt catttcgtga
    10321 gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg
    10381 aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc ttcttcacat
    10441 accggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc acggaactgt
    10501 ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac
    10561 atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg
    10621 caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga
    10681 gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca
    10741 tgaattgtag ccattagcat gcttccagtt ttctcagtca gagactgagc gttaatcgaa
    10801 attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt
    10861 gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg
    10921 taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg
    10981 acacagttgc tcaagtgttt cgatatcgcc cggctgacct tcaccacgct ccagcgcacg
    11041 cagaattttc acgctccacg gcagaccgtc gcggcacggc gtacaccagc cgcaggattc
    11101 acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac
    11161 cgccatcgcc agcgccgtac ccagacggct tcctgcttta ccgatacttt cgaattccat
    11221 cggcaggtca agatgcgctt cggtcaggaa gtcggtgccc gcgccgcctg gctgccaggc
    11281 tttgaatttc agaccgtcac gcataccacc ggcgtaatct tcgaggatct cgcgcgcggt
    11341 agtaccaaac ggcagttccc acaggcccgg atttttcacc cgaccggaga agcccatcag
    11401 cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag
    11461 gatcgccgga acgttacaca gggtttcgac gttgttgaca caggtcggtt taccccatgc
    11521 gccggaggtc gccgggaatg gaggcttcga gcgtgggtta gcacgacgtc cttccaggga
    11581 gttgattaac gctgtttctt ccccgcagat gtagcgccct gccccggtat ggacgaacag
    11641 ttcgaagtca aaacctgttc ccataatatt tttgccaagc agacctgctt cggtggcttc
    11701 ggcaatggca cggcgtagat tcactgccgc ttcgatatat tcgccacgca ggaagatgta
    11761 gccacggtaa gctttcagcg caaacgcgga gatgagcata ccttccacca gcaggtgcgg
    11821 cagttgctcc atcaacaggc ggtctttata ggtgcccggc tccatttcat cggcgttaca
    11881 cagcaggtaa cggatgttca tggattcgtc tttcggcatc aggctccact tcaggccagt
    11941 cgaaaagccc gcgccgccgc gccctttcag accagcgtct tttacctgat taacgatttc
    12001 gtccggagac agcccggtca gcgccttacg cgccccttcg taaccatttt tgctgcggta
    12061 ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc
    12121 gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg
    12181 gtcagatgcg cgtgagtgtc ctcatcgatc atcatatttg gccctttatc acagttcccc
    12241 aggcagcagg ttggcagcag cgtaaagcga ccgtcaaacg tcgtttgccc tggtttgatg
    12301 ttcagttttt tctccagcgc cgcctgaata ccctggtaac cgttgatgtg acagaccacg
    12361 ctgtcgcaat aacggatcac atggcgacca accggttggc ggaagatctg gctatagaac
    12421 gttgccacgc cttcgacatc gctcgccgga atacccagca catcggcgat cgcgtggatc
    12481 gcgccgtccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc
    12541 gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc
    12601 agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg
    12661 acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga
    12721 tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt gcggatacga gtgcgataac
    12781 tcatggtgct accgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaatca
    12841 tctggaaaga ttcattggca ggcatcaccg gcccccacga cacttgcagg aagtgggtga
    12901 tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat
    12961 ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg cgcagactct
    13021 ggcgcagctc ttccactttc agcattacgc gggtgtagca gtcagaaaca ccaccaccaa
    13081 ccgggatttc aaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga
    13141 agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc gcctctttcg
    13201 cgccataggc ggcaacgccc tgggaacgac ctttcagaat ggtattttgc agcgccgctt
    13261 tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc
    13321 agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgga
    13381 aacccgtgat cgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca
    13441 cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat gtacagcagg tggctgttga
    13501 tacggaacag ttcggagagc ataacgcgaa tgacgttaac gcgatccggc acggtgatcc
    13561 cggccagttt ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt
    13621 caatacggtc ggtatacgga atgtagctgt gccaggactg gcgctcgccc attttctccg
    13681 caccacggtg gtggtagccg atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt
    13741 gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca
    13801 tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg
    13861 cttccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc
    13921 gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc
    13981 gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca cgctcatacc
    14041 agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg
    14101 ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa
    14161 aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt
    14221 caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgccaact tccagtaatt
    14281 gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat
    14341 ccggcccaaa acggttgcgc agttcgccaa ttaccggatc atcaagatga tcgcgggtct
    14401 gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt
    14461 ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaaatcc
    14521 ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggtt acggcaatac
    14581 gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc
    14641 caaccaccca ggagagcgga cgacgttctt tgccgataga ttcctgcaac agcatcagtg
    14701 cctgcatgta cgcttcagga cgcggcgggc agcccgggat atacacatca accgggatga
    14761 atttatcgac gccctgcaca acggaataaa tatcgtacat accaccagag ttggcacagg
    14821 cacccattga gataacccat tttggttcca gcatctggtc atacagacgc tgaataaccg
    14881 gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag
    14941 cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca
    15001 tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc
    15061 agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag
    15121 ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta
    15181 tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac
    15241 tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc
    15301 caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca
    15361 caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt
    15421 caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc
    15481 gggcggagcc gaccgagtcg ataccggatt caaacggcac gtttttcgac ctcgcgcgtg
    15541 cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga
    15601 taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca
    15661 ttgcttactc atcaaaagta gcgccagatt cactgctctt cacggcagat ggacgccaca
    15721 tcgattcatg gggaggaata aaaaaaacct tacaatcact gtagaaattc ttttatacag
    15781 ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa caaaagtttc
    15841 ttcacattag tttacataat atcaacacca ttagcattta atgccctttc accccagatc
    15901 cttgacgact ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa
    15961 aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt
    16021 tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa cgcagcaaca aatttacgta
    16081 ttttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gttcgggacg
    16141 tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt ctgcactatg cgaggaagga
    16201 tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt tttttacact
    16261 attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca atcaacaatg
    16321 aatcatcccc ttccggagca gacattgggc tgtattgcca cggattatgg tagctctcca
    16381 tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag cacagcaaat
    16441 attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc aggtccgggc
    16501 tcatcatctc aaccggcctt gccgtcacgc caagaccggc tttcactgcc gcacgaacgg
    16561 ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctgcttta ttaagcgtcg
    16621 ccagcaccat atcgcgaaac ggacttggat catccagcaa tacaagaggg atcggctcgc
    16681 ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggtt cgcaggttca
    16741 atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa atccacttcc tgggattcca
    16801 gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctaat ttcggataaa
    16861 ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca tctgaagcac
    16921 cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc tcatcattaa
    16981 aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt aacagtttgt
    17041 tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc tgcatttgct
    17101 gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa gtgttcagat
    17161 cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc ggacgatttg
    17221 cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat gctgtgcaca
    17281 cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg gaacccggac
    17341 gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc cgttataatg
    17401 cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt tactcactct
    17461 tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt gttccactct
    17521 cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgag gcggcatcaa
    17581 gagcagggat ccgtcattta tgcgagcatt ttaccccaaa aaccttttat ttataagggt
    17641 cattgcgaat tatctgatgc aaagtt
//
LOCUS       DAZFDP010000101        17612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFDP010000101 DAZFDP010000000
VERSION     DAZFDP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3014)
                     /locus_tag="ACHJ1G_003390"
     CDS             complement(<1..3014)
                     /locus_tag="ACHJ1G_003390"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO4765187.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAID
                     DKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRGEGKETTRYTSNIAIRTCDDTNIIAMAHGQGGAEIKTDNSA
                     PTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDWHTLIPTEKRKKVNSDELAYLHN
                     HYPDLSEEEAAMLAADGPCYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVK
                     PAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQENLLNDVN
                     ADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFL
                     NRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAA
                     GEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGA
                     PNIRELYHDSGFRVEPLFARRSVSCKGDTRGVA"
     gene            complement(3011..3916)
                     /gene="rdgC"
                     /locus_tag="ACHJ1G_003391"
     CDS             complement(3011..3916)
                     /gene="rdgC"
                     /locus_tag="ACHJ1G_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4765188.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            complement(3909..4193)
                     /locus_tag="ACHJ1G_003392"
     CDS             complement(3909..4193)
                     /locus_tag="ACHJ1G_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765189.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGINRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            4656..5444
                     /locus_tag="ACHJ1G_003393"
     CDS             4656..5444
                     /locus_tag="ACHJ1G_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765190.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQALNTGSESS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAR"
     gene            5484..5906
                     /locus_tag="ACHJ1G_003394"
     CDS             5484..5906
                     /locus_tag="ACHJ1G_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HFO4765191.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            5932..6072
                     /locus_tag="ACHJ1G_003395"
     CDS             5932..6072
                     /locus_tag="ACHJ1G_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765192.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            6084..6476
                     /locus_tag="ACHJ1G_003396"
     CDS             6084..6476
                     /locus_tag="ACHJ1G_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765193.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            6810..7694
                     /locus_tag="ACHJ1G_003397"
     CDS             6810..7694
                     /locus_tag="ACHJ1G_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4765194.1"
                     /translation="MLEENKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            7957..8292
                     /locus_tag="ACHJ1G_003398"
     CDS             7957..8292
                     /locus_tag="ACHJ1G_003398"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO4765195.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIK"
     gene            8395..8766
                     /locus_tag="ACHJ1G_003399"
     CDS             8395..8766
                     /locus_tag="ACHJ1G_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765196.1"
                     /translation="MAYVSISGYEPDAIRAQCELLKAMFMLRTKYPWETGRVCFPMEE
                     LTATIDSQLYHDNPEKFFSLLAGNGLNCVSGLGRSRIQHASLFGPFFYDRHGYFEAEL
                     PALTRAIDTIDFERLFAALSK"
     gene            8935..10131
                     /locus_tag="ACHJ1G_003400"
     CDS             8935..10131
                     /locus_tag="ACHJ1G_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4765197.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            10148..11149
                     /locus_tag="ACHJ1G_003401"
     CDS             10148..11149
                     /locus_tag="ACHJ1G_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO4765198.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            11374..13080
                     /locus_tag="ACHJ1G_003402"
     CDS             11374..13080
                     /locus_tag="ACHJ1G_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765199.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFNV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            13141..14730
                     /locus_tag="ACHJ1G_003403"
     CDS             13141..14730
                     /locus_tag="ACHJ1G_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765200.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYKDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            14740..15555
                     /locus_tag="ACHJ1G_003404"
     CDS             14740..15555
                     /locus_tag="ACHJ1G_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765201.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            15708..16172
                     /locus_tag="ACHJ1G_003405"
     CDS             15708..16172
                     /locus_tag="ACHJ1G_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765202.1"
                     /translation="MNMAQNWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMV
                     WEGGTPPTFTLPVTFIALFDPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIG
                     RQINIIDVAIQDISFDLDAPRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            16184..16693
                     /locus_tag="ACHJ1G_003406"
     CDS             16184..16693
                     /locus_tag="ACHJ1G_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO4765203.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTVAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(17241..>17612)
                     /locus_tag="ACHJ1G_003407"
     CDS             complement(17241..>17612)
                     /locus_tag="ACHJ1G_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4765204.1"
                     /translation="SNITFKGLPVRLRYSPEYDGKEFGFWHLVSEGKIEEERIPDLER
                     CKRIRWIAHMIRNYNHSDISCWSERRGPTEEWVIWNECENYVVVLSARRDYWLLKTAY
                     VVTYNSKIRTLKQSRKRALGT"
BASE COUNT         4746 a         4126 c         4103 g         4637 t
ORIGIN      
        1 gctacacctc gagtgtcccc cttacaagac acagaacgtc tggcaaaaag aggttccact
       61 ctgaagccac tgtcatgata aagctctctg atgtttggcg cgccactgtt agtaatgaga
      121 acctttgcac ctcgacgatg agcatccgtc aacagagaca ccaggcgttt ttgctcttca
      181 aacttaaagt catgaccgga atagttcgtg aatccctctg tatttggaag cggttcatac
      241 ggcggatcgc aaaagatgac atctccttct ccggcagctt caatcaccgc tgcaaaatca
      301 ccgcatacaa actcagaccg cccttccgca ccgaggaagg cttccatctc ctgtaatggg
      361 aaatacggag ttttatactt cccataaccg acattgaact caccggcctg gttgtaacgc
      421 gtcaatccgt taaaacaatg tcggttcagg aacaaaaacg ccgctgcgcg atgtaaatca
      481 tcatagactt gtttgttaaa cgcattccgt actgccaggt atccttcctg tgtgttgtag
      541 tcctggaaga aacgatgtgc cagagtgata agtgaatgcg cctcgcgttg cagagtcttg
      601 taaaagttaa tcaggtcagc attcacatca tttagcagat tttcctggta tccggcattc
      661 atgaagacag ctccgccacc aacgaaaggt tcaatcaggc gcttcccttc tggcaaatag
      721 cgaaagattt gttccagaac accaaatttt ccaccagccc atttgaatat ggaccgttcg
      781 aattctgccg ctggtttaac ttttcgctct tttgtttcac ttccttcttt ctgccgacat
      841 acggccttag taatccgatc gccaatccag cgcattactg gtattgccat actattgccg
      901 atcgctttgt aacacggtcc gtcagctgca agcattgcgg cctcttcttc gcttaaatct
      961 ggatagtgat tgtgcagata tgccagttca tctgaattaa cttttttacg cttttccgta
     1021 gggatcaacg tatgccaatc aggaaaacct tgcagccttt cacattcgac aggggtaaga
     1081 cggcgcattc taccgtcgcc gagcaataca attggtgctt catggttaca tgtcaaagtt
     1141 ggtgccgaat tatcggtttt tatctcagcc cctccttgcc catgtgccat ggcaattatg
     1201 tttgtatcat cgcaagttct gatagcgatg tttgaagtat atctggtagt ttccttcccc
     1261 tcgcctctgc tcggcgcaat attccggcgc acgccttcga actcaaaaag taccgttgcg
     1321 ggatcgagat ctgttcgagc acttgcgaca acaaacacgc gtcggcgtcg ttgtgccact
     1381 ccgaagtatt gggcatcaag gattctccag gccacttttc gctgcggtcc ataaatacaa
     1441 ccacactgcg gccactttgg aacatggcaa ccggttttgc catcccaccg ccagaacgcg
     1501 ttgctttttc ctgattcagg tcgatcacct ggttcaaatg gcacatcttc tccagccaat
     1561 ccggcaagga aacatccgaa ggcgttatct gccgatgaca agactcctgg gacattttcc
     1621 cagacgataa cggctggttt gagaaatgac tcagcccgtt tgtcgtcaat tgcatttgca
     1681 agctccacat actttaaagt tagcgcgcca cgctcatcat caagcccacc acgtaatccc
     1741 gcgatactga atgcctgaca gttatggacc accgcaccat tgaggatata ggaatgatca
     1801 ccttcgactt ctatgttgta tacagtatct aggcctaccg atttaaactc tttgactgtt
     1861 cgtaaaagca ttccatgagc caatcttgat ttacgtgaca atttctgcgg gcagattgtt
     1921 acctgataat aattccgttg atttaccacg cgatcctcga tcactttttt gggctcaact
     1981 tcaataaagc tgaccgaaga aacataacca caagtctgtg acaaccccgc aacgccccaa
     2041 gcaagcgcag aactaacact attaattcta aatcccgctt taccacttgg tgtcccatca
     2101 gtatcaagat agccttgtaa aaacacatga cgcaatggat gcgacatcac ccatgcaggg
     2161 atacgcttag catggcttaa ctcgccaaaa tgttcattaa gccaattggc ataacacgta
     2221 tcattcaagg ttactttgat gctccctcgg atttcgcttg ccacggaaaa tatgttttct
     2281 ggtatgcggc aatgaaactt tctcaatttc tggcaattta tgccaaaaac aaccgccttc
     2341 ttagatttac ctctccatct cctaatatat ccatcgccaa cataagcgcc cgcaagatac
     2401 atagcctgtt cttcagacaa gaaccgagaa caaatatctg gaaatgcaat attgaaatta
     2461 gttagagcgc accattgata tcccggcata tcacatgctg ctcgccattc cggttcagac
     2521 aacaactctc tcttaaaata tgtgccattt ttccgggtgt tttgggcttt ccaccgaaca
     2581 gccaggaagg gatggtcatt ggttgttctt atacctaaag gctgcccaac ggcattaagt
     2641 aaccccgtat tagctatttt tgaaccaact ctttttactt gttgtaaccg cccgagatga
     2701 ctgactacgt aatcaccagg gcaaacattt tctattggtt tatagccatt tttacaaaga
     2761 accatatgcc ccgcggtaaa acatggtgtt cccccgacga gcacgtcagg ggattcgatt
     2821 tccccagcca agactttttt ggcaagtttt ttcatgtcgc caaggttggc aacatggggc
     2881 cagcggagcg caagaacggc cgatggaaaa ggctcgattt cagcaaacca cgccggacgc
     2941 atacccaacg gttcccaggc aatactcgcg gcttcaattc cactgcaaac agatccatag
     3001 cacagctctt tcactgctta gcctctccgc caagggcatt taccagagca tcaaccaggc
     3061 acgaaatttc actggtcaac aggaagaaat ctgcgtccag tcgctgcgca acatcttcac
     3121 tatcaatatc agagttctgc tcaagcaatt catccgcaaa tttgacgctg gtaaggctga
     3181 agttatggtc cagtgtaaat ttaatgcggt tctgccagtc gagtgccaac ttagtgacga
     3241 gcttgccagc ttccaggtgt gtggaaattt catcgcttcc caaatcctgc tttttcactc
     3301 gggcaatacc gccatcctca agcactgcct taagttctgc cgcatccccc atttgaaatc
     3361 cctgtggagc actaccatca cgtacccagt cggtcagcgt taattcaatg ggattttcaa
     3421 cactcagggg aacagcagga agagaaccca gagacttacg cataagcgcg agcatatcct
     3481 ctgcctgccg cgcgctggca ttgatataga tacgtttagt tgaaccgtcg tagatcgcct
     3541 ggataacaga aaactttgaa aaagcccgtg gcagaagaga atgcagaact tcgtctttca
     3601 gggagtcctt ctctgttttc ttcagtttac gcgcttgttc ttgctcaagt ttttcaattt
     3661 tttcttgaat agctcgctgg ataaccggcg ggggaagaat ttttgtttcg cgctttgctt
     3721 caacaaggat aaaaccattt ccatgcatag cgataacttc agaattatca ccaaatggcg
     3781 atacaaaacc gaacttggcc atatcctgac taccgcatgg cgtgaaaagg atcattttct
     3841 ttttatcttc taagtcggtc agatccgcct cacgagaaag tttataaata gtaatgtttt
     3901 tccagtgctt aaacatgttg taacccttga atatcaacca cagaaagctc gtctttgtag
     3961 aaaaaggcca ggttgtggca ccccctcgtt tgagcgtatg agctgggacc aatttcgttc
     4021 ttccagacaa atggcttcaa atccgtacgg cgaagcataa aaacgcgatt tattccgctc
     4081 tgattcccaa tgaggcaaaa gccttctttc accttgatag cctgcaagtt gtcgagttca
     4141 ccgctggtta cacggctatc gaactctttg cggcttatta gctccatctg catctgacga
     4201 ctccaaacaa atgcccattg aagggcgatg gctgaatggt accgaaaata cgacataaaa
     4261 aacaatattt attagagcaa ttttgcgata agtaaacgcc atacagacca caaataacct
     4321 aagttaaaat aacgaaaatc agagcaaata attggtgatg gcgtggcaag cattgcaaca
     4381 aaagacagca tttgttcggg gcacggagga ttcccatcca ggcctcctgt agagagtgaa
     4441 ccactactta aagtcaacgg agtcgaagtg ttagttgatg gtaagcaata tgcacagcat
     4501 accgatggaa acagtacgca cggtgggcaa gctatatcaa ccagggcatg gtttaccgtc
     4561 aatggtaaag ggatcgtatg cgttggtgac cctgtttcat gcggatctac cgtagcgtcc
     4621 ggagacggcc tggttcaggt aagttaggag atatcatgct ggaaaaagac taccagttat
     4681 ccgcatataa aaaattggcc gccgccggtg ggatgaaaac acctggtgcc ataacatcgg
     4741 cacgaaacag tgctaacaca gcaaaactgc ttgcagaaga attgaccgga ttaattctgg
     4801 atacaattgt ctatcccgac actattacca gctatgtttc aacgatcaga acaaccacaa
     4861 ccggcttaac gaacattgga gaactggcaa ctaagcacgc ggacctgttg gctggttatg
     4921 cagatctgtc aatgctgctt caactcgata ttggttggga tgtttactgc cgtgctaatg
     4981 agcgagaagt ttcagaactg ccgatctcta ttgccattgg tgatgtgaat attactaaat
     5041 cgcttgagga cgctgttaac gcgcttaata catcaagttt agtcgctgca atgggggaga
     5101 ttaaccaggc ccttaacact ggctcagaaa gctcgtcagg ctctggttca ggcggcggca
     5161 ctgccactcc cccaccagca ctaacagaag agcaaattga atctctgaaa gtagcaactg
     5221 aacagtttgg ggttgttttc aaccagacaa cagcacccac aactgcgtta caacagcagt
     5281 atgaacgagc gaatgaaagc gccaacgtag ccataactgc ttataaccat gctatcggta
     5341 ccgcgcttgc ggaagcatca gcaaataagg ccagcacagc cagcgcagtc gccgctttgg
     5401 ttcctgattc tgttcttgat gaattaaaca aagcggcacg gtaacaaagg acttcattga
     5461 taatttttct tcaggaggaa gacatgtcat tcttttctac gttaaaaaca gctttgtctt
     5521 tgaaggagaa acttgctgct actggtgttc ttgttctgat ttgcgcactt gttggtgctg
     5581 ggtttgcatg ggaacgtcat cagctaaagc aagccatgga gaaaattggc agtcttgatc
     5641 aggctgttaa ggaacgtgat aagtcaataa tggatcttaa ccagaccatt gagacgatga
     5701 acaaagcaga gcaacatttt cacagccagg aagtgaaaaa tgaatcagaa caagccaaat
     5761 atgctgacag gcaaatggaa cgaaaagctg aagttcagaa acaactggtt gcggcgggta
     5821 atgttcgcca gcgcattcct gctgacactc agcggttgct ccgggagtcg atcagcgaat
     5881 ttaacgccga cgccgacaaa ggttaaccac cctgccccca aaagtgcgtt tatgtgcagg
     5941 atgccagagt ttagcagtga atattttgat gatctgccag cgtatatcct cgatacagaa
     6001 acgatgctga tggggattaa caggaagaat cgcaacgtta atgattacaa ccgcgctatc
     6061 agcggtaact aaaagggatt tttatgtctg ataaagtaac agtaaagcaa actatcaaca
     6121 aagcgacttc aatttacaaa attgagcaca tcactgttgg caagccagga tctgaacaat
     6181 accgtcatgc tttcgagctt gccgatcagc ttggtttaaa acacccggat tgcatcgagc
     6241 atgtatttcc gacctatgct gatgagcaat gtactcatgt tcttaccgaa gaggattttt
     6301 tcagcactga agaacgagaa ggcgttgatc gctgcattgg tgtgatttgc tcttcagtga
     6361 gttatgagtt attccctaat gtccatgaaa atggtggtat tggataccaa ttcctgtacg
     6421 aaggcgatga gcttaaatgt tatgaacatg gtcttcttat cgaaagcgta gaataatacc
     6481 cttccttcca accggctatg ttggccggtt tattcaactt atccacagca tagatccaat
     6541 aaacagatcc taaagagaac ccaggaagat ccaaagaaga tcccggatcg ctgtaagccg
     6601 cgccatttct ggcctgaaat gggatcaaca ttgactatac gcgattttat gttgactgtg
     6661 cacgatttat tgttgactgc acgcgattta ttgttgacta tatgcgacag aaacattgac
     6721 tgtacgcgat tttagagcct gactattcac agttgttgat aactgcaatc cagatgacgc
     6781 caggccgcgc cacatatgga gaaaccacga tgctggaaga aaataaaggc ttccttagcg
     6841 ttgaagaagt tgcaggaaat acaggagaaa tccacagcct gaaacccaat aacaatagca
     6901 ctatacaacc cattgctttg ttgcgcttag gtgtgtttgt gccaacctta aaatctacca
     6961 atgtggcact acgtcgcgga tcgtcagtta ctacaaacac aacgaacgca accgaagaac
     7021 tatcaagcct caaaattgtt gagcaggaag gctatgaggg aattgaaatt catggtccac
     7081 gcctggatat ggatactgat tttaaggtgt gggtgggcat aacctccgcg ttgtttgact
     7141 acgcacctga tgatgacggc ataatcaccc tgccattctc cgagtttgcc gatcgatgcg
     7201 gctatccacg taagcgcctt tcaaaggcgt tccgtaaaag tattgatgac tctctgacac
     7261 gcattcagca gacagttgtc aaattccgct tcccggcggc aaaaggtcat ctcaataaca
     7321 ttaacgtcaa cttgttggca tatagcagcc tgaataccga gcttgatgtt atcgagatcc
     7381 agccgcagaa acagctatct gaactttact atgttgacta taagcgaatc ctgaagctga
     7441 agatgctgga taagctcggg cgcaaagaga cggccaaggt actgtataca ttctttgagg
     7501 ctctacccgc caacccggca cctgtcagca ttgagcgcct tagagcaagg cttaatctca
     7561 aatcatccgt tagcgtgcaa aatagcgtta tcagaaaagc catgaaagat ttggaagcta
     7621 ttgaatatct taaattttca gagataaaaa acggcaggaa aatcggcttt cagatccata
     7681 agcgcaatcc ataatattga ctatatgcga tagcgagaag ttgactatag gcgacattcg
     7741 ttgacgctgg tggatttttg ctggcgtcaa tattctgcaa gtcgctattg agatggcttt
     7801 tagggtcatt tcatcgcgta tagtcaacgt ttctcccgac aatatcttac atagtcgatc
     7861 tttggtggag ttaaatcgac tacagtcaac ttttgactgt agtcacatcg cgcatagtca
     7921 acatttgcgc ggttctcatc aagcagtggt attgatatgc aagaagagaa acaacactac
     7981 ctctacgttc tggtgccgga gaacggagat acttttaaaa tcggcatttc atgtggtcca
     8041 ttggcacggt ttaaagggct acaagtgagt cccgattttg cgctttcacg ggtctatcgt
     8101 ggtacgcgtt tggcaatggt taatcttgag cgggctttac acgcaacctt tttcccctgg
     8161 aatgcgccgt gggagaaaag cgccggtggc gggcatactg aatggtttac acgagagtgt
     8221 cttgataagg ttttggctca tatcgaatat ctaaatgata tgtggggagg gattctcgag
     8281 cgcattaagt agaatgattt acttcagcgt ccagtagatg ctgctcgctc tttcgaaaaa
     8341 gaactggatg ttacttctat cgtgactttc aaagatgacg caggaatgag ggacgtggct
     8401 tatgtctcca tatctggcta tgaaccggac gcgatccgcg ctcaatgtga attgctgaaa
     8461 gcaatgttta tgcttcggac taaatatccc tgggagacag ggcgggtgtg cttccctatg
     8521 gaagagttaa ccgccaccat tgattctcag ctttaccacg ataatccaga gaagtttttt
     8581 agccttttag ccggtaatgg gcttaactgt gtgtccgggc tggggcgaag tagaatccaa
     8641 catgcctcgc tcttcggtcc cttcttttac gatcgacacg ggtactttga ggctgaactt
     8701 ccggcgctta cgcgtgctat agatactatc gatttcgaac gattattcgc tgctcttagc
     8761 aaataacact gatgcccctg aacggggctt ttttgtgcct ttcttgtaac tctcaatcgt
     8821 gcaaaatgaa caaaacatgc agagaatgcc atgtacaagc atctacgcat acattattat
     8881 tttatgcagc atttttaatt aaattcaaaa atacagcata aaggatgact ttcgatgagt
     8941 gattccagcc agcttcacaa ggttgctcaa agagcaaaca gaatgctcaa tgttctgact
     9001 gaacaagtac agttgcaaaa ggatgagcta cacgcgaacg agttttacca ggtctatgcg
     9061 aaagcggcac tggcaaaatt gcctctactg actcgagcga acgttgacta tgccgtaagt
     9121 gaaatggaag aaaagggtta tgttttcgat aaacgccctg ctggctcttc aatgaaatat
     9181 gcgatgtcaa ttcagaacat cattgacata tatgaacatc gcggagtgcc aaaataccgg
     9241 gatcgctaca gcgaagcgta tgtgattttc atctccaatc ttaaaggcgg tgtgtcaaaa
     9301 actgtatcga cggtttctct ggcgcatgca atgcgtgctc accctcatct tctgatggaa
     9361 gatttaagga ttctggttat tgaccttgat ccgcaatctt cagcaacgat gtttttaagc
     9421 cataaacact ctattggtat cgtaaacgca acatctgcac aggctatgtt gcagaatgta
     9481 agccgtgaag agctgttaga ggagtttatt gttccttctg ttgtacctgg ggttgacgtt
     9541 atgcctgcgt cgattgacga tgcctttatt gcatccgatt ggagagagct gtgcaatgag
     9601 catctaccgg gtcagaacat ccatgctgtc ctgaaagaaa atgtgattga taagctgaag
     9661 agcgattatg actttatcct cgttgatagt ggtcctcacc ttgacgcctt cctgaaaaat
     9721 gctttggcct cggccaatat actgtttaca cctctgccgc ctgcaacggt tgatttccac
     9781 tcatcgctta aatacgttgc ccgccttcct gagttggtga aactcatttc ggatgaaggc
     9841 tgcgagtgcc agcttgcgac taacattggt tttatgtcca agttgagtaa caaggcagac
     9901 cataagtatt gccatagcct ggctaaagaa gtgttcggtg gggatatgct tgatgtcgtc
     9961 ctccctcgcc ttgacggttt tgaacgctgc ggcgagtctt ttgacactgt tatttcagct
    10021 aaccctgcaa cgtatgttgg tagtgctgat gcattgaaga acgcgcgaat tgccgcggaa
    10081 gattttgcta aagcagtttt tgaccgtatt gaatttatca gatctaactg aggagtaaga
    10141 aacccccatg tcaaagaaaa acagaccaac aattgggcga acccttaatc cttcaatatt
    10201 aagcggattt gatagttctt cagcctctgg cgatcgagtc gagcaggtat tcaagttatc
    10261 aactggtcgc caggccacat ttattgaaga ggtaatacct ccgaaccagg tagaaagcga
    10321 tacctttgtt gatcagcata acaacgggcg tgaccaggca tctcttacgc caaaatcatt
    10381 aaaaagtatc cgaagcacta ttaagcatca gcaattttac cctgcaatag gtgttagacg
    10441 ggctacaggg aaaattgaaa ttttggatgg ttcccggcgt cgagcttctg ccatcttaga
    10501 gaacgtaggg ttgcgggttt tagtcacgga ccaggagatc agcgttcagg aagcgcaaaa
    10561 tttagcgaaa gacgttcaga cagcattgca gcacagcatt cgagaaatag gtctgcgttt
    10621 gatgcgaatg aaaaatgatg ggatgagtca gaaggatatt gcagccaaag aagggctgtc
    10681 tcaggcgaag gtcacgcgtg ctctccaggc agcgagtgct ccggaagaat tagtcgccct
    10741 tttccctgtg cagtcggaat taaccttttc ggactacaaa acgctttgtg ctgttggcga
    10801 cgaaatgggg aacaagaatt tagagtttga tcagcttatt caaaacatat ccccggaaat
    10861 aaacgacatc ttatccattg aagaaatggc cgaagatgaa gttaaaaata aaatcctgcg
    10921 cttgataaca aaggaagcct cactactcac ggataaaggt tctaaagata agtccgtagt
    10981 tactgaatta tggaaatttg aggacaagga tcgctttgca aggaagcgcg tgaaaggccg
    11041 tgcattttct tatgagttta atcgactttc aaaagagcta caggaagaac tcgacaggat
    11101 gattgggcat atccttagaa agagcctcga taaaaagccg aagccttaaa ctttcgccat
    11161 tcaaatttca ctattaacct actgttttta aagtaaatcc atctaaaatt tcaaggtgaa
    11221 atcgccacga tttcaccttg gattttacct tcctccccta ctcccgaaaa aataaaaaaa
    11281 ttgcttgtca cgagaaagtc aacaagtgac tttcaataaa atctcttccg aaaagggatt
    11341 cacacaagtg ccttgtgttt aaggaagagt aaattgagta acttacgcga ataccagaat
    11401 cgtattgcag atatcgcaaa acgctctaaa gctgtgcttg gctgggcaag cactgcgcag
    11461 ttcggtactg ataaccaatt cattaaagat gatgccgcgc gtgccgcatc tatccttgaa
    11521 gctgcacgta aagacccggt ttttgcgggt atctctgata atgccaccgc tcaaatcgct
    11581 acagcgtggg caagtgcact ggctgactac gccgcagcac ataaatctat gccgcgtccg
    11641 gaaattctgg cctcctgcca ccagacgctg gaaaactgcc tgattgagtc cacccgcaat
    11701 agcatggatg ccactaataa agcgatgctg gaatccgtcg cagcagagat gatgagcgtt
    11761 tctgacggtg ttatgcgtct gcctttattc ctcgcgatga tcctgcctgt tcagttgggg
    11821 gcagctaccg ctgatgcgtg taccttcatt ccggttacgc gtgaccagtc cgacatctat
    11881 gaagtcttta acgtggcagg ttcctctttt ggttcttatg ctgctggtga tgttctggac
    11941 atgcaatccg ttggtgtgta cagccagtta cgtcgccgct atgtgctggt ggcaagctcc
    12001 gatggcacca gcaaaaccgc aaccttcaag atggaagact tcgaaggcca gaatgtacca
    12061 atccgaaaag gtcgcactaa catctacgtt aaccgtatta agtctgttgt tgataacggt
    12121 tccggcagcc tacttcactc gtttactaat gctgctggtg agcaaatcac tgttacctgc
    12181 tctctgaact acaacattgg tcagattgcc ctgtcgttct ccaaagcgcc ggataaaggc
    12241 actgagatcg caattgagac ggaaatcaat attgaagccg cacctgagct gatcccgctg
    12301 atcaaccacg aaatgaagaa atacaccctg ttcccaagtc agttcgttat cgcggctgag
    12361 cacacggtac aggcggcgta tgaagcacag cgtgaatttg gtctggacct gggttcccta
    12421 cagttccgca ccctgaagga atacctgtct catgaacagg atatgctgcg tcttcgcatc
    12481 atgatctggc gtactcttgc gaccgacacc tttgacatcg ctctgccggt taaccagtcc
    12541 tttaatgtat gggcaaccat cattcgtggc aaattccaga ctgtatatcg cgacattatt
    12601 gagcgcgtta aatcttctgg tgcgatgggg atgtttgctg gtgctgatgc agcatctttc
    12661 ttcaaacagt tgccgaagga tttcttccag ccagccgaag actatatcca gactccgtat
    12721 gttcactaca tcggtaccct gttcggtaac gtgaaagtgt acgaagtacc tgctggtatt
    12781 tgtaagaact taacgacaga gaacattcag ttcagctcga tggatgtgct gtgctacgtc
    12841 cgtgatgaaa atccgggtaa agcaggcttc gtgactggtg atgctgtccc ggctatcccg
    12901 ttccagcatc cgaccactcc ggcgctggtc aaccgtacca cactgtgggg ttcggctatc
    12961 aacgatatgc acccacgcaa cggcgctgat tacttcactc gtgtaacgct gacaatggcc
    13021 aaaaaaggcg ggcttaactt cataagcggc gacacgattg atgccggtga ctctgagtaa
    13081 tcaggggaag ttctccgttt aacatagcgc ccccgtgcgg ggcgcataac agggaaagtt
    13141 atgtctcaat attcaattca acagtcatta ggtaatgcat ccggcgtcgc ggttagcccg
    13201 atcaatgccg atgcgacgtt atctaccggt gttgcattaa atagcagctt gtgggctggt
    13261 attggcgtat ttgcgcgtgg caagccgttt actgttcttg cggttactga gtccaattac
    13321 aaagatgttc tcggcgaacc gctgaagccg tcttccggct cacagttcga accaattcgc
    13381 catgtatacg aagctattca gcaaacgtca ggttatgttg ttcgtgctgt tccggatgat
    13441 gcgaagttcc cgattattat gttcgatgaa tcaggcgaac cggcttacag tgcgttgcca
    13501 tacggttctg aaattgaact tgatagtggc gaagcctttg ctatctacgt tgatgatggt
    13561 gatccgtgta tttcacctac ccgtgagtta accatcgaaa cggcaacagc ggacagcgcg
    13621 ggtaatgaac gcttcctctt aaaactgacc cagacgactt cgctcggcgt ggtaacgacc
    13681 ctggagacac acactgtgtc tttggcggaa gaagcgaaag atgacatggg ccgcttgtgt
    13741 tatctgccta cggctctgga agcccgttct aaatatctgc gcgcggttgt taatgaagag
    13801 ctgatttcga cggcgaaagt aacaaataaa aaatcgctgg cgttcactgg tggtaccaac
    13861 ggtgatcagt cgaaaatctc aaccgctgcg tacctgcgtg cggttaaggt gctgaacaat
    13921 gcgccgtaca tgtacaccgc tgttctcggc ctgggttgct atgacaatgc ggcgatcacc
    13981 gcgttaggta atatctgttc tgatcgcctg attgatggct tctttgatgt caaaccgaca
    14041 ttgacgtata cggaagcgct ctctgctgtt gaagataccg gtttacttgg taccgattat
    14101 gtaagctgtg ctgtctatca ctacccgttc tcctgcaaag acaaatggac ccaatcccgt
    14161 gtggtctttg gtctgtctgg cgtggcgtat gcggcgaaag ctcgtggcgt caagaaaaac
    14221 tctgatgtcg gcggttggca ttactcaccg gctggtgaag aacgtgccgt cattgctcgt
    14281 gcgtcaattc aaccgctgta tccggaagat accccggacg aagaagcaat ggtcaagggc
    14341 cgtctcaata aagtatctgt tggcacctct ggccagatga tcatcgacga tgctttaact
    14401 tgctgcacgc aggataacta tctgcacttc cagcacgtcc catccctgat gaatgcaatc
    14461 agccgtttct ttgtccagtt agcccgacag atgaagcata gcccggacgg tattactgcg
    14521 gctggcctga ctaaagggat gaccaaactt ttagatcgct ttgtcgcctc cggcgctctg
    14581 gtggctcctc gtgatcctga tgctgacggt acagaaccgt atgtgctgaa agttacgcag
    14641 gcggaattcg ataaatggga agtagtctgg gcctgctgcc cgactggcgt agcccgtcgt
    14701 atccagggcg taccgctgct tattaagtaa gggaatacaa tgagcaaaaa cttttttcaa
    14761 tccggggcat ttttggggaa tggactgtct cgtttcgctt tgaactctga tcctgtgcag
    14821 ctgatggagt ctgcccgagc aagcgccgaa cagccaacag atccggttat taataataat
    14881 ccggaaccgg cggcacagac taacgataac gttccatctg ccccggctcc tgagcaaatc
    14941 ctggaaggga aagacggtaa agaatggacc gtcgaacagg cgcaccagat gattctggaa
    15001 gctgcaaata gaagtgctat gcagaatgcg ttgagtgatg cggccgacgc cgttttcgcc
    15061 tgggctgata gcggtgatct gactttcgac tcccttgatg gtttcgttca ggctatcgct
    15121 ggtatctctg atgacgacga ctccgaagtt acagaagaac aggacgatgc ctataacgaa
    15181 gcatgggcaa atgttgctga cttcctcgca gcatgcggtg tagatgatga cctgatcgaa
    15241 gcactggctg acgatgaaga cgacgacgca gctgctgatg ttggtgcctc tatcgctggt
    15301 ttagatagcg acgaccgtga cgaactggaa gcggcgtttg ttgttgctgg cacttctgat
    15361 gaaatgctga ctgaagcatt taagaaggtt gttcgtaacg gtgagatcaa actcatccgt
    15421 aaacgcctgc gtaaaaaacg tctgactgcg gctcaaaaat cggcgctgaa aaaagcgcgt
    15481 cgaaaagccc agacgggtgc ggcaaaactt gcccgcaaaa agtcaatgaa actgcgccgt
    15541 aagcgccttg gctaaaggag gaggccggag aactccggcc tttaacttga atggcaccta
    15601 taccttatgg ggtttacagc caggctgacg gtgtatcgcc atttctgaaa gttactttaa
    15661 cgaactctca gtaccaggtt accggatata tcagccaggg ggcagcaatg aacatggccc
    15721 agaattggga agcgccgttt accggtatgt ccatggggtc tgttgctggt gccttcagtg
    15781 gttttgcgca ggttggtact gaaacaacgt cggtggcccg ttggaacagc ttaatggttt
    15841 gggagggggg aacaccgcca actttcacgc tgccagtaac tttcatcgct ttgtttgatc
    15901 cattcacgga ggtttcagga gctatcgccg cattgtcagc gatgattagc ccggaactta
    15961 aagatgccag tattggtggt cgaatcccgg agcgtgtgac gctaaacatt ggtcgccaga
    16021 tcaacatcat tgatgtcgct atccaggaca taagtttcga tctcgatgcg cccagggaca
    16081 gcaatgggca tttcctgaaa aacaccgtca acctccagtt gaccggttct tcgatatata
    16141 acagctccga tattgttcgg gcgttccagt aaaaggattt tatatggggc acaataacac
    16201 taagggaaac cgtaaattta ttaagggccg ctatactgcc aacgcggcca aaggcgaacg
    16261 actggtatct tctgaattcc agctcactgt tgcaggccat gaagatatca gcgtactggt
    16321 tcgcacgtcg caaattcctg aaatgacccg cgaggatgtg gaggactatg gtccgaatgg
    16381 tgtgaagttc aaccagcacg gtccaattcg aaactctggg gaaatccagg tccagtgcgt
    16441 ggagactatc gaaggcgata ttcttcagtt cattaaagat cgcattgcgg cgaaggacta
    16501 tgttgatatc acgatggctg ctacccctga atccaaatct tccggggtta acgctgtgac
    16561 aaaagctgct acaacaattg aaatgttgga ctgcaaaatc tacagtgatg caatcgactt
    16621 tagtaccgaa gatgtgactg ccgctgtgcg cccgtcactt cgtatcgttt acaactggat
    16681 tgagtgggat taagagtcat cccttgtatt ttaaagctcc tgcgggagct ttttttgtgc
    16741 ttaaattatg ctggtatagc gaaattttga gcatattatg gaaattgatt tttcatactc
    16801 tcctgaaacg atagaacgaa gattcgaaat aattggatgc aaaactattt cagaggagca
    16861 ttattggatt ctctatgatg ccaatacatg gttatgtgcc ttagcagaat gtcggtcatc
    16921 gttatgtgca ggggagggag gacttcgaca taaggtgctg gctacacttg aagtgaacac
    16981 gttacgatat tggtgtgtcg aaatacttag gaataacaaa gaattacatt tattattgct
    17041 gaataaatgt gcacctctgc gcagaaaagc ataagaaaac ccaacagcca tactggaatt
    17101 atcagaagaa tatgtgcgga aagttatagc tagctattga aaagctaaag tagatcggtt
    17161 agatttgcct tactcatcta ccatgtgtag aaagagcgat cgaacccgat acatagcaat
    17221 atgtgtcggg ttcagctttt ttatgtccca agcgctcttt ttctgctttg tttgagtgtt
    17281 ctgattttac tgttataggt tacaacatag gctgttttaa gaagccaata gtctctacgt
    17341 gcggatagca caacaacgta gttttcacac tcgttccaga ttacccattc ttcagttggt
    17401 cctcgtcttt cagaccaaca tgatatatcg gaatggttat aattccttat catatgcgcg
    17461 atccagcgga tccgcttaca ccgttcaaga tcaggtattc gttcttcttc tatttttccc
    17521 tctgatacta aatgccaaaa tccgaactct ttcccatcat attccggtga ataacgtaac
    17581 cgaacaggga gacccttaaa cgttatatta ga
//
LOCUS       DAZFDP010000102        17543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFDP010000102 DAZFDP010000000
VERSION     DAZFDP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..1019)
                     /locus_tag="ACHJ1G_003408"
     CDS             complement(156..1019)
                     /locus_tag="ACHJ1G_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4765205.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="ACHJ1G_003409"
     CDS             complement(991..2541)
                     /locus_tag="ACHJ1G_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO4765206.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="ACHJ1G_003410"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="ACHJ1G_003410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4765207.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="ACHJ1G_003411"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="ACHJ1G_003411"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4765208.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="ACHJ1G_003412"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="ACHJ1G_003412"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4765209.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6382..7101
                     /gene="deoD"
                     /locus_tag="ACHJ1G_003413"
     CDS             6382..7101
                     /gene="deoD"
                     /locus_tag="ACHJ1G_003413"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4765210.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7262..7525)
                     /locus_tag="ACHJ1G_003414"
     CDS             complement(7262..7525)
                     /locus_tag="ACHJ1G_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4765211.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHJ1G_003415"
     CDS             complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHJ1G_003415"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4765212.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(8601..9245)
                     /locus_tag="ACHJ1G_003416"
     CDS             complement(8601..9245)
                     /locus_tag="ACHJ1G_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO4765213.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            9351..10319
                     /gene="serB"
                     /locus_tag="ACHJ1G_003417"
     CDS             9351..10319
                     /gene="serB"
                     /locus_tag="ACHJ1G_003417"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4765214.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            10368..11750
                     /gene="radA"
                     /locus_tag="ACHJ1G_003418"
     CDS             10368..11750
                     /gene="radA"
                     /locus_tag="ACHJ1G_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4765215.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            11771..13003
                     /gene="nadR"
                     /locus_tag="ACHJ1G_003419"
     CDS             11771..13003
                     /gene="nadR"
                     /locus_tag="ACHJ1G_003419"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4765216.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHJ1G_003420"
     CDS             complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHJ1G_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4765217.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            15188..17125
                     /gene="sltY"
                     /locus_tag="ACHJ1G_003421"
     CDS             15188..17125
                     /gene="sltY"
                     /locus_tag="ACHJ1G_003421"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4765218.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            17215..17541
                     /gene="trpR"
                     /locus_tag="ACHJ1G_003422"
     CDS             17215..17541
                     /gene="trpR"
                     /locus_tag="ACHJ1G_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4765219.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         4018 a         4574 c         4892 g         4059 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
     3001 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg ttcccgtacc
     3121 gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
     3301 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt gctgactggg
     3481 ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact
     3961 cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
     4021 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
     4441 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag agggtgaaaa
     6361 catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
     6421 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc tgaaactttc
     6481 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
     6541 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
     6601 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
     6661 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
     6721 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
     6781 atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgtgggtaac
     6841 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
     6901 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc tgcagagttt
     6961 ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc
     7021 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
     7081 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg ttaagccgga
     7141 gcaggagact gctccggctt tttagtatct attcatcaat agcaactgat ttatagattt
     7201 actattattt ttcatatgga tagaaaataa aatccattca agtatctatt cacgaatcta
     7261 ttcatttatg ttcagccgtc atccaggaag ggcatatcag caactctggt ggacaaccat
     7321 aaatttctgc cattttgctt ttgatgtttg agctaagccg ttccgtcttt tcgatctgtt
     7381 ttagagaatt aatcgagata cctaacattt tacttacttc agcaagcgat aatttatgcc
     7441 tgatacgcac ggcggctagc caggaaatac gctggcttaa cattaaattg ataatctccc
     7501 gctcctggtt ttccagatcg atcatattcc cttcctttgg tattttaatc agcgagttac
     7561 cttaccgccc ccgctatcca cgtcgacaac tcccgtagct ctttttcctg gtccgggaag
     7621 tcaaccaaca gcgcttcgca ctcttgttgc agcatatccg cgcggtacag gcagccttgc
     7681 agtcgcccgg cgagggcttc cagcggcgcg gggttgaggc tgtcggtaaa cacctgggcg
     7741 cgggtgatat ggcctttttc aacgtcgaaa tgcagttcca cgccgcccca gctaaagcgt
     7801 tcatccagca gatgcgagaa tgccggagcc tgaccgaagt tccattccca gctactctga
     7861 cgggcaaagg tttcggcgaa gtttggcaag tctggcgttt tgtccgggga gatgatttcc
     7921 gcttctacac gctcgccata atgggcgaaa aaggccttgg ttatgacctc gcaaacctgc
     7981 tcatgggtga tccccggcag cagctcggtg aggttggtca cgcgggaacg cactgaggta
     8041 atgcctttcg ccgccagttt ctttttatcc ggattgagat agtttgccag gcggctaagg
     8101 tcggcattga gcagcaaagt gccgtggtgg aagccacgat ctttggtttc gcgataggcc
     8161 gagcctgaga ctttgcggtc gccttcgact gttttcacca ccagatcgtt acgcccggac
     8221 gcttcggcgc tgacgccgag cgcgttcagc gcattgagca caatcgacgt agagatggtt
     8281 ttatcgtact ccggcttgcc agccataaag gtaaagcagg tattgccgag atcgtggaac
     8341 accgcgccgc caccgctact gcgccgcgcc aggcggacgt tatcttcttc catccgccgg
     8401 gtattacact ctttccacgg gttctgcgcg cgaccaatca ctaccgtgtc ggcattgcgc
     8461 cagagaaaca gaacgcgctg cgtggcgggc atttggcgaa aaatacactc ttccaccgcc
     8521 aggttaaacc acgggtcgta agagtcagag atgagcaggc gtaatgtgga cataacgatt
     8581 tcctttcttg tagtaatggg tcactctttt ttctcgcttt cttcctcttc cggcaccggt
     8641 ttgctggcgg ttaacaggaa gggcgattgc tgccagcggg tgcgtttacc ctgtagcagc
     8701 gtgcgggtca gcactacgcc gattgccagt gagagcagca acatcaggcg taaaatgtta
     8761 gtggtgttat ccacctgttg ggcttcggtg gcgagcgtat gggtgtcgag tgtcaggcgc
     8821 agatagccga gcggtccgtt tttacccgca attggctcga caatctgctg gttaaaatag
     8881 ccgcctgctt ttttaccgtc gagcgccagc cggtcgcgca cttcgacgct ttcgcctgaa
     8941 cgggcgataa gatcgccttg ttcgtcatac acacccgcgt cgaggatgcg gctttcatcc
     9001 gttaactgat cgaggatcgc ctgaatgcgt ttttcatctg gtgagtcggt acgcatcagc
     9061 ggtgcaacgt tcagcgtcac ctgacgcgcc agggtgcggg ccagttcttc cagctgtgga
     9121 ttacgctgtc gctggtggtt ttgactaaac catgacgccc cctgcatcag tgccactaac
     9181 aaggcaagac agaacaggac aatcactgcc cgatgcagcc ggaatttcag ttttgtgcga
     9241 gccatcttcc accctttgaa aatttgagac ttaatgttgc cagaagcaat ggatacaagg
     9301 tagcctcatg cgttattttc cctgcttcga acgattttac aggagcctta atgcctaaca
     9361 ttacctggtg cgacctgcct gaagatgtct ctttatggcc gggtctgcct ctttcattaa
     9421 gtggtgatga agtgatgcca ctggattacc acgcaggtcg tagcggctgg ctgctgtatg
     9481 gtcgtgggct ggataaacaa cgcctgaccc aataccagag caaactgggc gcggcgatgg
     9541 tgattgttgc cgcctggtgc gtggaagatt atcaggtgat tcgtctggca ggttcactca
     9601 ccgcgcgcgc tacgcgcctg gcccatgaag cgcaactgga tgtcgcgccg ctggggaaaa
     9661 tcccgcacct gcgcacgccg ggtttgctgg tgatggacat ggattccacc gccatccaga
     9721 ttgaatgtat tgatgaaatt gccaaactgg ccggaacggg cgagatggtg gcggaggtaa
     9781 ccgaacgggc gatgcgcggc gaactcgatt ttaccgccag cctgcgcagc cgcgtggcga
     9841 cgctgaaagg cgctgatgcc aatattctgc aacaggtgcg tgaaaatctg ccgctgatgc
     9901 caggcttaac gcagctggtg ctcaagctgg aaacgctggg ctggaaagtg gcgattgcct
     9961 ccggcggctt tactttcttt gctgaatacc tgcgcgacaa gctgcgcctg accgccgtgg
    10021 tagccaatga actggagatc atggacggta aatttaccgg caatgtgatc ggcgacatcg
    10081 ttgacgcgca gtacaaagcg aaaactctga cccgcctcgc gcaggagtat gaaatcccgc
    10141 tggcgcagac cgtggcgatt ggcgatggag ccaatgacct gccgatgatc aaagcggcag
    10201 ggctggggat tgcctaccat gccaagccaa aagtgaatga aaagacggaa gtcaccatcc
    10261 gtcacgctga cctgatgggg gtattctgca tcctctctgg cagcctgaat cagaagtaat
    10321 tgcctgcccg tcatcctgcg ggcggcacag cattaacgag gtacaccgtg gcaaaagctc
    10381 caaaacgcgc ctttgtttgt aatgaatgcg gggccgatta tccgcgctgg caggggcagt
    10441 gcagtgcctg tcatgcctgg aacaccatca ccgaggtgcg tcttgctgcg tcgccaacgg
    10501 tggcgcgtaa cgagcgtctc agtggctatg ccggtagcgc cggggtggca aaagtccaga
    10561 aactctccga catcagcctt gaagagctgc cgcgtttttc caccggattt aaagagttcg
    10621 accgcgtatt aggcggcggc gtggtaccgg gaagtgccat tctgattggc ggtaacccag
    10681 gcgcggggaa atccacgctg ttgctgcaaa cgctgtgcaa actggcccag cagatgaaaa
    10741 cgctgtatgt caccggcgaa gagtcgctgc aacaggtggc aatgcgcgct catcgccttg
    10801 gcctgccgac tgacaatctc aatatgttgt cggaaaccag catcgagcag atctgcctga
    10861 ttgctgaaga agagcaaccg aagctgatgg taattgactc catccaggtg atgcatatgg
    10921 cggatgtaca gtcatcgcct ggtagcgtgg cgcaggtgcg tgaaacggcg gcttatttga
    10981 cacgcttcgc caaaacgcgc ggtgtggcga ttgtcatggt tggacacgta accaaagatg
    11041 gctcgctggc gggccctaaa gtgctggaac actgtatcga ctgttcggtg cttttggatg
    11101 gcgatgccga ctcccgtttt cgcaccttgc gcagccataa aaaccgcttc ggcgcggtga
    11161 atgagctggg cgtcttcgcg atgaccgaac aggggctgcg tgaagtcagc aacccttcgg
    11221 caattttctt aagtcgcgga gatgaagtga cctccggcag ctccgtgatg gtggtgtggg
    11281 aaggaacgcg tccgttgctg gtggagattc aggcgctggt cgatcactcg atgatggcga
    11341 atccgcgccg cgtggcggta ggtctggagc aaaaccgtct ggcaatcctg ctggctgtct
    11401 tgcaccgtca cggtggtctg caaatggccg atcaggatgt atttgtgaac gtggtcggtg
    11461 gtgtgaaggt aaccgaaacc agtgccgact tagcgttact gctggcgatg gtttccagcc
    11521 tgcgtgacag accgctaccg caggatctgg tggtgtttgg tgaagtcggg ctggcagggg
    11581 agatccgccc ggtgcccagc ggtcaggagc gaatctctga agcggcgaaa cacggttttc
    11641 gccgggcgat tgttccggcg gctaacgtgc cgaaaaaagc gccggaaggg atgcagattt
    11701 ttggcgttaa aaaactctcc gacgcgctta gcgtgttcga cgacttataa tgagagatac
    11761 ggagggagat atgtcgtcat ttgattacct gaaaactgcc atcaagcaac agggctgcac
    11821 gttacagcag gtagctgatg ccagcggtat gaccaaaggg tatttaagcc agttactgaa
    11881 tgccaaaatt aaaagcccca gcgcgcaaaa gctggaggcg ttgcaccgtt ttttggggct
    11941 tgaatttccc cggcagaaga aaaccattgg tgtcgtattc ggtaagttct acccgctgca
    12001 taccggacat atctacctta tccagcgcgc ctgtagccag gttgacgagc tgcatatcat
    12061 tatgggtttt gacgataccc gcgaccgcgc gttgtttgaa gacagcgcca tgtcgcagca
    12121 gcccacggtg ccggatcgtc tgcgctggtt attgcaaact tttaaatatc agaaaaatat
    12181 tcgcattcat gctttcaacg aagagggcat ggagccgtat ccgcatggct gggatgtgtg
    12241 gagcaacggc atcaaaaagt ttatggctga aaaggggatc cagccggacc tgatctacac
    12301 ctcggaagaa gccgatgcgc cacagtatat ggaacatctg gggatcgaga cggtactggt
    12361 cgatccgaaa cgtaccttta tgagtatcag cggtgcgcag atccgcgaaa acccgttccg
    12421 ctactgggaa tatattccta cggaagtgaa gccgttcttt gtgcgtaccg tggcgatcct
    12481 tggcggcgag tcgagcggta aatccaccct ggtaaacaaa cttgccaata tcttcaatac
    12541 caccagtgcg tgggaatatg gtcgcgatta tgtcttttca cacctcggcg gtgatgagat
    12601 cgcattgcag tattccgact acgataaaat cgcgctgggc cacgcacaat acattgattt
    12661 tgcagtgaaa tacgccaata aagtggcgtt tattgacact gattttgtca ctacccaggc
    12721 gttctgcaaa aagtacgaag ggcgtgagca tccgttcgtg caggcgttga ttgatgaata
    12781 ccgtttcgat ctggtgatcc tgctggagaa caacacgccg tgggtggcgg atggtttacg
    12841 cagcctcggc agttcggtgg atcgcaaaga gttccagaac ttgctggtgg agatgctgga
    12901 agagaacaat atcgaattcg tgcgggttga agaggacgat tatgacagcc gtttcctgcg
    12961 ctgcgtggag ctggtgcggg agatgatggg ggagcagaga taaccgcgat gaaacggctc
    13021 aaaggcgagg tataaaataa gttttttgat gagttgaaaa cctttctgga agaggctgtc
    13081 gagattaaac aaggtttgaa aaaacctgca cgggtgaccc gctacgaaat tgcggatact
    13141 aaggctgttg taggccggaa aaggcgttca cgccgtatcc ggcattcggt gctcaatgcc
    13201 tgatgcgacg cttaccgcgt cttatcaggc ctggagattc acaacgtatt gaaatttctt
    13261 ggtttgtagg ccggataagg cgttcacgcc gcatccggca ttttacgcat tacttcgcaa
    13321 tacgcttgta cttgatacgc ttcggctcca gtgcgtctgc gcccagcgtg cgtttcttgt
    13381 actcttcgta ctcagtaaag ttaccttcga agaactcaac tttaccttca tcctggtagt
    13441 ccaggatgtg cgtggcgata cggtcgagga accaacggtc gtgcgagata accatcgcac
    13501 agcccgggaa ctccagcagg gcgttttcca gcgcgcgcag ggtttcgata tccaggtcgt
    13561 tggttggttc gtcgagcagc agcatgttgc cgccaacctg cagcagcttc gccagatgca
    13621 gacgaccgcg ctcaccaccg gaaagttcac caacgcgttt accctgatca acccctttaa
    13681 agttaaagcg gccaacgtag gcgcggcttg gcatctcggt gttgccgatc ttcataatat
    13741 ccagcccgcc ggaaacttct tcccaaacgg ttttgctgtt atccattgag tcacggaact
    13801 gatcaaccga tgccagtttc accgtttcac ctaaagtgat ggtgccgctg tccggctgtt
    13861 cctgaccaga gatcatacgg aacagggtcg atttacccgc gccgttcgga ccgatgatcc
    13921 cgacgattgc ccctttcggg atcgagaagc tcaggtcatc aatcagcagg cgatcaccgt
    13981 aggatttacg caggttgctg acttccagca ctttatcgcc cagacgcggt ccaggtggaa
    14041 taaacagttc gttggtttcg ttacgtttct gatattcggt gctgttcagc tcttcaaagc
    14101 gtgccagacg tgctttacct ttcgactggc ggcctttagt tccctgacgt acccactcca
    14161 gctctttctc gatcgactta cgacgcgccg cttcttgtga agcttcctgc gccaggcgct
    14221 gatctttctg ctccagccag gaggagtagt tgccttccca cggaatacct tcaccgcggt
    14281 caagttcgag gatccagcct gcaacgttat cgaggaagta acggtcgtgg gtaatcgcca
    14341 ccacggtgcc ctcgaagtcg tgcaggaagc gttccagcca ggccacggat tcggcatcca
    14401 ggtggttggt cggttcgtcg agcagcagca tgtctggttt ttccagcagc aggcggcaca
    14461 acgctacgcg acgacgctca ccaccggaga ggttagcgat tttcgcgtcc cagtccggca
    14521 gacgtagcgc atccgccgca cgctccagct gtacgttcag gttatgaccg tcgtgagcct
    14581 gaatgatctc ttccagacgg ccttgttcag cggccagctt gtcaaaatcg gcatccggat
    14641 cggcgtacag cgcatacact tcatccaggc gtttcagggc gttaaccact tcagaaaccg
    14701 cttcttcaat ggactcacgc acggtgtgtt ccgggttcag ctgcggttcc tgcggcaggt
    14761 aaccaatctt gatgtctggc tgcggacgcg cttcaccttc gatgtcttta tcaatgcccg
    14821 ccataatgcg cagcagggta gacttacccg cgccgttcag acccaggaca ccaatttttg
    14881 ccccagggaa gaaactcaga gagatgtttt tcaaaatatg acgtttcggc ggaacaactt
    14941 tgccgacacg atgcatggta taaacgaatt gagccacgtt ggacttcgcc tctatgttta
    15001 tcgtgatgat gagttttcaa aggcgaagtg tagccttttt ccccgcctaa tcccagttgg
    15061 tcgatcacct tcgcagtaaa agtaaaaaag tgtccgtaac gtggcgtaaa cggcaatgac
    15121 tggttagcat aaatctatta cgcggcatga cgctgcattg atgtatttac acttagagga
    15181 tgcgcttgtg gaaaaagcca aacaagttac ctggcggctg ttggctgccg gtgtctgtct
    15241 gctgacggtc agcagcgtgg cgcgagccga ctcactggat gagcagcgta gccgttacgc
    15301 gcaaatcaag caggcctggg ataatcgaca aatggatgtg gtcgaacaaa tgatgcctgg
    15361 actgaaggat tatccgcttt atccctacct ggaataccgc cagatcaccg atgatctgat
    15421 gaatcaaccg gcggtgacgg tcactaactt tgttcgcgct aaccccacgc ttcctcccgc
    15481 tcgcacgctg caatctcgtt tcgtcaatga actggcgcgg cgtgaagact ggcgtggctt
    15541 gttagccttt agcccggaaa agcccggaac taccgaagcg caatgtaatt actactatgc
    15601 gaaatggaac accgggcaga gtgaagaagc ctggcaaggg gcgaaagagc tgtggctaac
    15661 cggcaagagc cagcctaacg cctgtgacaa gttatttagc gtctggcgtg cgtcaggtaa
    15721 acaagatccg ctggcttatt tagagcgtat ccgtctggcg atgaaagcgg gtaacactgg
    15781 cctggtaaca gtgctggcag ggcagatgcc tgccgattac cagactatcg cctcggcaat
    15841 catttcactg gcgaacaacc ctaatacggt actgaccttc gcgcgtacaa ccggagcgac
    15901 cgattttacc cgtcaaatgg cggcggtggc gtttgccagt gtggcgcggc aggatgctga
    15961 aaatgcacgg ctgatgatcc cgtcgcttgc ccaggcgcag cagcttaatg aagatcatat
    16021 tcaggagctg cgcgatatcg tcgcctggcg tttgatgggc aacgatgtca ccgacgagca
    16081 ggcgaaatgg cgcgatgacg ccattatgcg ctcgcaatcc acgtcgttga ttgaacgccg
    16141 tgtgcgaatg gcacttggca ccggcgatcg tcgcggcctg aatacctggc tggcgcgtct
    16201 gccgatggaa gcaaaagaga aagatgaatg gcgttactgg caggcggatt tattgctgga
    16261 acgcggacgt gaagctgaag cgaaagagat tttgcatcaa ctcatgcaac agcgtggttt
    16321 ctacccgatg gttgcagcac aacgcatcgg agaagagtat gagctgaaga ttgataaagc
    16381 gccgcagaat gttgacagcg ccctgactca ggggccggag atggcgcgcg tgcgcgagtt
    16441 gatgtactgg aatctcgata acaccgcgcg tagcgagtgg gccaatctgg tgaagagcaa
    16501 gtcaaaaaca gagcaggctc aactggctcg gtatgctttc aataaccaat ggtgggatct
    16561 tagcgttcag gcaacgatcg ccgggaagct ttgggatcat ctggaagagc gattcccgct
    16621 ggcttacaac gatcttttca aacgttacac cagtgggaaa gagatcccgc aaagctatgc
    16681 gatggcgatt gcccgtcagg agagcgcctg gaatccgaaa gtgaaatcac cggtaggggc
    16741 cagcggcctg atgcagatta tgcctggtac agcgacccat acggtgaaga tgttctctat
    16801 tcctggttat agcagccctg ggcaattgct ggatccggaa acgaatatca acattggcac
    16861 cagttacctg caatatgttt atcagcagtt tggcaataac cgtattttct cctcagcagc
    16921 ttataacgcc ggacctggac gggtgcgaac ctggcttggc aacagcgccg ggcgtatcga
    16981 cgctgtggca tttgtcgaga gtattccgtt ctccgagacg cgcggttatg tgaagaacgt
    17041 gctggcttat gacgcttact accgctattt catgggggat aaaccgacgt tgatgagcac
    17101 cacggaatgg ggacgtcgtt actgatccgc acgtttatga tatgctatcg tactctttag
    17161 cgagtacaac cgggggaggc attttgcttc ccccgctaac aatggcgaca tattatggcc
    17221 caacaatcac cctattcagc agcgatggca gaacagcgtc accaggagtg gttacgtttt
    17281 gtcgacctgc ttaagaatgc ctaccaaaac gatctccatt taccgttgtt aaacctgatg
    17341 ctgacgccag atgagcgcga agcgttgggg actcgcgtgc gtattgtcga agagctgttg
    17401 cgcggcgaaa tgagccagcg tgagttaaaa aatgaactcg gcgcgggcat cgcgacgatt
    17461 acgcgtggat ctaacagcct gaaagccgcg cccgttgagc tgcgccagtg gctggaagag
    17521 gtgttgctga aaagcgattg att
//
LOCUS       DAZFDP010000103        17441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFDP010000103 DAZFDP010000000
VERSION     DAZFDP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHJ1G_003423"
     CDS             complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHJ1G_003423"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4765220.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1007..3448)
                     /gene="rnr"
                     /locus_tag="ACHJ1G_003424"
     CDS             complement(1007..3448)
                     /gene="rnr"
                     /locus_tag="ACHJ1G_003424"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4765221.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3487..3912)
                     /gene="nsrR"
                     /locus_tag="ACHJ1G_003425"
     CDS             complement(3487..3912)
                     /gene="nsrR"
                     /locus_tag="ACHJ1G_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4765222.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4117..5415)
                     /gene="purA"
                     /locus_tag="ACHJ1G_003426"
     CDS             complement(4117..5415)
                     /gene="purA"
                     /locus_tag="ACHJ1G_003426"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4765223.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5519..5716)
                     /gene="yjeT"
                     /locus_tag="ACHJ1G_003427"
     CDS             complement(5519..5716)
                     /gene="yjeT"
                     /locus_tag="ACHJ1G_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO4765224.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5798..6802)
                     /gene="hflC"
                     /locus_tag="ACHJ1G_003428"
     CDS             complement(5798..6802)
                     /gene="hflC"
                     /locus_tag="ACHJ1G_003428"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4765225.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6805..8064)
                     /gene="hflK"
                     /locus_tag="ACHJ1G_003429"
     CDS             complement(6805..8064)
                     /gene="hflK"
                     /locus_tag="ACHJ1G_003429"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4765226.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8150..9430)
                     /gene="hflX"
                     /locus_tag="ACHJ1G_003430"
     CDS             complement(8150..9430)
                     /gene="hflX"
                     /locus_tag="ACHJ1G_003430"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4765227.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9507..9815)
                     /gene="hfq"
                     /locus_tag="ACHJ1G_003431"
     CDS             complement(9507..9815)
                     /gene="hfq"
                     /locus_tag="ACHJ1G_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4765228.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9901..10851)
                     /gene="miaA"
                     /locus_tag="ACHJ1G_003432"
     CDS             complement(9901..10851)
                     /gene="miaA"
                     /locus_tag="ACHJ1G_003432"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4765229.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10844..12691)
                     /gene="mutL"
                     /locus_tag="ACHJ1G_003433"
     CDS             complement(10844..12691)
                     /gene="mutL"
                     /locus_tag="ACHJ1G_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4765230.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12701..14038)
                     /gene="amiB"
                     /locus_tag="ACHJ1G_003434"
     CDS             complement(12701..14038)
                     /gene="amiB"
                     /locus_tag="ACHJ1G_003434"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4765231.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14057..14518)
                     /gene="tsaE"
                     /locus_tag="ACHJ1G_003435"
     CDS             complement(14057..14518)
                     /gene="tsaE"
                     /locus_tag="ACHJ1G_003435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4765232.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14490..16037)
                     /gene="nnr"
                     /locus_tag="ACHJ1G_003436"
     CDS             complement(14490..16037)
                     /gene="nnr"
                     /locus_tag="ACHJ1G_003436"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4765233.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16036..17175
                     /gene="queG"
                     /locus_tag="ACHJ1G_003437"
     CDS             16036..17175
                     /gene="queG"
                     /locus_tag="ACHJ1G_003437"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4765234.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17158..17211)
                     /locus_tag="ACHJ1G_003438"
     CDS             complement(17158..17211)
                     /locus_tag="ACHJ1G_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765235.1"
                     /translation="MRFYFYSQAVDESGVTW"
BASE COUNT         3960 a         4980 c         4372 g         4129 t
ORIGIN      
        1 taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
       61 tccaaatctg gatggctttt cataattcag acgaactagc tgcgctgacg aaccgcttca
      121 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
      181 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
      241 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
      301 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
      361 ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgta
      421 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc
      481 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
      541 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct
      601 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
      661 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
      721 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
      781 tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt
      841 gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg
      901 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
      961 gcattttgtc agcaatctaa ccctcttctt ttaaagaggg tattgatcac tctgccactt
     1021 ttttcttcgc cgcacgcttc gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg
     1081 gctttttcgc ttttctcgcg tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc
     1141 ggaaggcgct gtctggctca aagtttacct ttttaccgac ctgacgacgc ttgccgcctt
     1201 ttttgcctgc atcgcctttt ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg
     1261 cgcgttcact ggagatcaga ctaaagtcga ttttgcgctc gtccatatta accgcttcga
     1321 ctcgaacttc cacgcgatcg cccaggcgat aagtctggcc gctggattcc cccatcaggc
     1381 gttgccctac ctggtcaaag cgatagtagt cattgtccag cgaagagaca tggaccagac
     1441 catcaatgaa caagtcgtcc agacggacga agaagccaaa gccagtgacg ctggaaatta
     1501 cgcctttaaa gacgttacct acctggtcga gcatgaagtc acacttcagc cagtcagcga
     1561 catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga acagtgctga cccagttgca
     1621 acatctcttc catcgaataa tggtagccgc cggtttcagt ggtgttgccc tgatgcccct
     1681 gctctttcgc cagcagatat ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa
     1741 tcggcgaagt aaagtgcgca taggactgca atgccagacc gaagtgacca cggttttctg
     1801 gatcgtaaat cgcctgtttc atcgagcgta gcagcatggt ttgcagcatt tctgcatcag
     1861 gacggtcggc aaccgactcc agcagttccg cgtagtcacg cggttccggc ttgttaccac
     1921 ccggcagctc cagccccagc tccgccagca ctgaacggaa agaggtaatc gcttcggtgc
     1981 tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc tttctcaacg aaacgcgccg
     2041 ccgagatatt cgccagaatc atgcactctt caattaattt gtgcgcgtcg ttacgctggg
     2101 tctgttcgat acgttcaata cggcgttcag cgttgaaaat gaacttcgct tcttcgctct
     2161 caaatgagat cccaccgcgt tcttcacggg ctttatccag cactttatag aggttatgca
     2221 actcttcgag atgcttaacc agcggggcgt actgctcgcg cagatcctga tcgccctgca
     2281 gaatatgcca gactttggtg taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga
     2341 atttgtagcc cgtcaggcgg cctttcgacg aaaccgtcat ctcgcacacc atacacaggc
     2401 ggtctacctg cgggttgagc gaacacaggc cgttagagag cacttccggc agcatcggga
     2461 taacctgcga agggaagtac accgacgtgc cacggttacg cgcttctctg tccagcggcg
     2521 ttggcggacg cacatagtag ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc
     2581 cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg
     2641 tgaccagcgg taaatcgcgc agatcaacac ggcccgcttt tgcttcttcc ggcacttctt
     2701 ctttcagccc ggcaacctgt tgctcaacag cctgcggcca gatgtacgga atttcatggg
     2761 tacgcagagc gatatcaacc gccatgccgg tgcccatatt gtcgcccagc acttcgacga
     2821 ttttacccac cgctttggtg cggcgagtcg gacgctgagt cagttcgact acgaccacaa
     2881 agcccatccg cgcgcccatg atctgatcgg gcgggattaa gatatcgaag ctcagacggc
     2941 tatcgtcagg aaccacaaag ccgacgcccg cttcagtaaa gtagcgacca acaatctggc
     3001 tggtttttgg caccagtacg cggacaatac gcgcttcacg acgaccttta cggtcagcgc
     3061 ccagcggctg cgccagcacc tgatcgccat gaatgcaggt tttcatctgc tcgctggaga
     3121 gatacaaatc atctttacgc ccttcaaccc gcagaaagcc gtagccatca cggtggccaa
     3181 taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc atagcactga cgacgagtga
     3241 agaccagttg accatcgcgc tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct
     3301 cttcgccttc aatgtgcagt tctaccgcca gctcatcacg gctggccggt ttttcacgtt
     3361 tggttaaatg ttcgaggata aattcccgac tagggatggg attcgcgtat ttttcagctt
     3421 cgcgttcctg gaaaggatct tgtgacatct cggttcctcc gttgtcatct ctgatgaaga
     3481 ttttcgtcac tccaccagca ataatttata aagcggttga ttctcttcaa ccaaatcggc
     3541 aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt
     3601 caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg caattcacca gcgataaggg
     3661 ctccagctcg cgcaccacat caccaatacg tatcgcactc gccggtttac ccaggcgaat
     3721 gccgccattt tttccacgaa cagcagtcac gtagccggca cgactaagtt gattgattat
     3781 tttgaccata tgattacggg agacgccgta gacgtcagtc acttcagaaa tactggtcat
     3841 ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt aatccgtaat cagtgaaact
     3901 cgttaactgc acatcaacct caaaagggaa atcgggaaaa aatacattta tattgatgat
     3961 aaaccagcca caagctgtgt cgctaattta tttcagttcg gggaaggaaa aaagcgagga
     4021 tttaacactg tgtcggatag cgtaatggct taaccgacac agcaatgtat cacactgtta
     4081 gcccggcagg caaaatatct gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag
     4141 aatcatggtt tcagtacgat ccggaccggt agagatgata tcgatcggca caccagtcag
     4201 ctcttcaata cgcttgatgt agttcagcgc cgcctgcggc agaccgctac gatctttcac
     4261 gccgaaggtg gattcagacc agcccggcat ggtttcgtaa atcggctcta cacctttcca
     4321 gtcgtcagct gccagcggag tggtcgtcac ttcacgacca tccggcatac ggtaagccac
     4381 acagagtttc acctctttca ggccatccag aacgtccagt ttagtcaggc agaagccaga
     4441 cagggagttc agctgtaccg cacgacgaac ggcaacggtg tccagccagc cggtacgacg
     4501 acgacgcccc gtagttgcgc cgaattcgtt accctgcttg cagaggaact cgccagtttc
     4561 atcaaacagt tcggtcggga acggacctgc acctacacga gttgagtaag ctttgaggat
     4621 acccagaaca taatcaacat aacgcgggcc caggccggaa ccggtcgcca cgccaccagc
     4681 agtggtgttg gaagaagtta cgtacggata agtaccgtgg tcgatatcca gcagcgtacc
     4741 ctgcgcacct tcaaacatga cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga
     4801 aacgtcaacc accatagaag tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt
     4861 ctggtaatca accgcttcag ctttgtagta gttaaccaac tggaagttgt gatattccat
     4921 cacttctttc agtttttcag cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc
     4981 gcgacgtgct actttatctt cataagcagg cccgatacca cgaccggtgg tgccgatcgc
     5041 tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca acgtgataat caaggatcag
     5101 cggacatgct tcagacagca gcagacgctc acgaacgggg atgccacggt cttccagttc
     5161 tttcatctct ttcatcagcg cggccggaga cagcacaaca ccgttaccga tgatgctggt
     5221 tacattctcg cggagaatac ctgatggaat aagatggaga acggtttttt caccgttgat
     5281 tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc
     5341 agtcagaaga tcgacgatct tacctttacc ttcgtcaccc cattgggtgc ccagtacgac
     5401 gacgttgtta cccatttttc aaaatcaccg tttgcttaaa aatggattct accatcgctt
     5461 tttcagagtt acagcacttt ttgcactcaa aaatgacctc gatcagtctg ttttttgttc
     5521 agccaatcgt tttcctcaac atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc
     5581 caaaacgacg taaaatatta tcgggcaaat tggtcatcgc agagatcatc ttcttccatg
     5641 ccttcgggta aagcatcggc cctaaacctt ccagtaccaa aaccagggca agcgccagcc
     5701 agattgtcga attcattcat tatccttata gaaaaagaaa accaccgaca tccctgagga
     5761 tgcggtggct ttattgacct gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg
     5821 tcttcatgta gcggaagaaa tcgctatccg ggctcatgac catcacgtcc tgattgccag
     5881 agaagctgtt ctcataagca cgcaggctac ggatgaatgc gtagaagtcc ggatctttac
     5941 tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc
     6001 cctgacgctc agcttctgcc agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt
     6061 tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc
     6121 gcatacggtt gtagatcgct tcagacactt cggtcggcag gttgatctgc ttgatacgca
     6181 catcgacaac ttcaataccc agcgccgcca tactgttcgg gttgatgacc ggaactttgc
     6241 ccttcgtctc agccgttacg cgctctgccg cttcggcaat ggcgttatct gctgccgggg
     6301 tagtaacttc atcttctgta cccgcagaac cggagttcag cgcgtcacgt acttcgaggg
     6361 tcagacgacc acgggaatca gtgacgatat ctttcacgtc caggcgacca atttcagaac
     6421 gcagacggtc agagaactta cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac
     6481 ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga
     6541 tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac
     6601 gtgcgtcgag cattttcacc gtttcaatga acggtatctt gaaatgcaga cccggctcat
     6661 aaaccagagg tttgttgtca tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc
     6721 gctcaccttc tttgacgaca aagacagaca tgtaaagcac taccagcacg atgatgataa
     6781 tcgcgataac tgacttacgc atcgttattc cccctgacgc tggtagtcgt tacgctgcgc
     6841 gttggcgcgg cgttggtcca taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc
     6901 actggttgtg gaggaagagg ctggcggcag acgcagcaga ttgctggcac cgttatcgct
     6961 cttcgccgca ggggcgttac cacctttcag catctggtct aacggcagaa ccatcaggtt
     7021 tccacctttg tcgttaacca gcactttgcg ggtgttgccc aacacttttt ccatcgtctc
     7081 gatatacaga cgctcgcgag taatttccgg cgcggcttta tattccggca gaagtttagc
     7141 aaagcgcgcc acttcaccct gagcttccag gatggtctgg gccttgtacg cacgcgcctc
     7201 ttcgaggata cgttgcgcct gaccgttcgc acgcggctga acttcgttgg tatacgcttc
     7261 tgcttcacga atgtattgct gttcgttttc acgcgcggca atcgcatcgt caaacgccgc
     7321 ttttacttct tccggcggac gagcagcctg gaagttgacg tccagcagcg tgatacccat
     7381 gtcatacgga cgaatcgtct cttccagttc gcgctgagta tcgctacgaa tcacggtacg
     7441 accttccgtc agaatgcggt ccatggtgta tttaccgata actccacgca gggcgctgtc
     7501 ggtagcctga cgcaggctgt catccgggct ggtcacgcta tacagatatt tttccggatt
     7561 ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg ttctcgtccg acgtcagcat
     7621 cacaccagaa gcggccagtt cacgcacggc ttccacgttc accggtttga cttcgtcgat
     7681 aaacgtcggt ttccagttca gacccggctc aaccagatgg ctgaatttac caaagcgtgt
     7741 taccacgccg cgttcggctt ctttaatggt atagaaacca ctggccgccc agataatgac
     7801 aatcgctgcc gctgcgatgg taacgacacg accgccaagc tgcgggcgcg ggccttgcga
     7861 tgaactgcca ccgccagatc cggtgccttt accgccgccc agaccaccga gctttttgct
     7921 cagtttgcgg aagatatcat ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc
     7981 atttccctca gagttgccgc caggtttgct gcttccccac gggtcgcggt cttgtccgtt
     8041 attaccgggc tgattccacg ccatgtttgt gctccatatt tgttatgcgg tgatccctgt
     8101 tgtctttcga cgccagagga tatgactcca cgcttcagac gctacgccgt tagatcaggt
     8161 aatcgatcaa cgccggttct tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa
     8221 cttgcagact tacgctgccg tcctcctcca tccactcttt ttctattgcc tgaagctgat
     8281 aaaaacgact tctcagacgc ccttcctgcg gtggcagacg caatgtatgc tgcgccacct
     8341 cgccggaaag ccgccccgtc aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg
     8401 cggaaagcca gacacggatc ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat
     8461 cttccagcat atcgatcttg ttcatcacca gcagggttgg gatctcgtga gcgtcgatct
     8521 cttcaagaac cgtattcacc gcttcgatgt tttcttgtac acgcacatcc gccgcatcaa
     8581 tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa cgtggcttta aatgccgcca
     8641 ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc tgcaagtacg gtttcaccga
     8701 catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg
     8761 cgtagacccg cgcttcggtg atgcgattga aaagggtaga tttaccggcg ttggtatatc
     8821 ccaccagcga aacagtagga acgtcggctt tgatacgcga ttgccgcccc tgctcacgct
     8881 gcttttcaac tctttccagg cgtgactgta tctgcacgat gcgattacgc aacaaacgac
     8941 ggtcggtttc gagctgggtt tcacccggac cacgcaaacc tatcccgcct ttctgtcttt
     9001 caaggtgggt ccagccacgc accaggcgcg tagccagatg gcgcagctgc gccagctcaa
     9061 cctgcaactt accctcatgg gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg
     9121 tgcggtcgat aacacgacac tcgcacaaac gctccaggtt acgctcttgc gccgggctca
     9181 gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa
     9241 ctgctttacc ttcacctaca aaatactttg ggtgcggcgc tttacggcta ccggtaatca
     9301 cctgcaatgc ttcgacaccg gcggaagaga ccagagattc aaactcctgg aggtcttcca
     9361 tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc ctgctcacca gcatcataac
     9421 ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg aacgcaggat cgctggctcc
     9481 ccgtgtaaaa aaaacagccc gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa
     9541 gtattctgcg cgctgctacc atgatggtag ttactgctgg taccgccacc ggcgttgtta
     9601 ctgtgatgag aaaccgggcg agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc
     9661 tggctgaccg tgtttttcaa caggatcacg aattgatcaa aagactcgat ttgcccttgc
     9721 agcttaatac cattcaccaa ataaatagaa actggaacac gttcccgacg cagtgcgttc
     9781 aggaacggat cttgtaaaga ttgcccctta gccattctct cttttcctta tatgcttatt
     9841 tgtactttga acctttcgat tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat
     9901 tcagcctgcg atagcaccaa caacctgtaa tacttcgtcg cgcgcctgtt ctggtttttc
     9961 actgtcaagc cagtgaaccc cttcccaacc acgcagccag gttatctgcc gcttcgccaa
    10021 ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg tatgagattt cgccttcaag
    10081 gtaagaccac atctggcgat aacccacgca acgaatggaa ggcaagtccg tatgcaaatc
    10141 tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa
    10201 acgctgctca atgcgttgat ggagcagttc acggctcgcc ggggcgatgg cgaactgatg
    10261 cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga
    10321 aatgaaaaaa acttccagtg cccgggaaag cctttgtgga tcatttggat gaatccttgc
    10381 tgccgcaacc ggatctacct cctgaagttg acgatgcaat gactcccaac cttgctctac
    10441 cgcctgttgc tcaattctgg cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc
    10501 ttccagcaat gccttgaaat acaacatcgt accgcccact aacagtggga tccgccccgc
    10561 cgcggtgata tcggccattt ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc
    10621 ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt
    10681 cggcttcgcc gtcccgatat ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa
    10741 ctctactggt aaaattttac gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg
    10801 ccccatcaaa aaaatcgcct taggcaggct cgccttactg atatcactca tctttcaggg
    10861 cttttatcgc cggatgtaaa tcaacagatt gtaacagacc acccggcggc gttttcacaa
    10921 gttgcggaca taaccgttcc acgtccgcca gcagggttat ggcctgtgcc attgaccact
    10981 gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc aatattgcca ggttcgaata
    11041 cggactgctt cgccaggtag cctatcagtt caggaatcaa gatttgtaaa ttttgttggc
    11101 gtaagggtaa aggcacggcc ctgatggtca catgctgtgc atctgactgg aaatcaatac
    11161 ccaattccgc cagggcagac tgcgcttttt ctaatgccga tttttcttcg ccagaaactt
    11221 ttagccgcaa cggaatcagc aacggctggg cgcaaactgg cgcttcaccc ggcgtcagtt
    11281 gcgcctgacg cagccaacgt tctgccactg gcaaggctaa aagtgaaatg ttgccatcgc
    11341 gctccagcaa cgcacagtcg gaatggacga tagtcagtac ccgaccaaaa ctctgactgt
    11401 tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg tttttgcatc ggcgcgggcg
    11461 tttgcaaaag ctggcgatac acttcacctt gctgtttctg gtagcctggc tgcgcattcg
    11521 gccagggggc agccggacga ctgcctgagg cgggcgcagg agagtagcgc ggagctaccg
    11581 gctcacgagc tgccggttct gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa
    11641 tgggacgcgg tgcaggttgg ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt
    11701 gtagcacgct cagcacgccc tgatagataa aatcatgcac cagacgcgac tgatggaaac
    11761 gcacttcgtg tttggcgggg tgtacgttga cgtccacctg atgcgggtcg atctccagat
    11821 acaacacaaa cgccggttgt tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg
    11881 catgattgat caggcgatcg cgcatcatgc gaccattcac gtagcaatac tgaatttccg
    11941 ccagtgcggg cgtagtgtga tttggatcgg ccacccagcc gcgcagcgtg agatcgccgt
    12001 gttgccattc aatcgccagc gcttgttcga gaaaagcggt gccacaaatc gcgcctaagc
    12061 gccgttcttt ttgcccgcct tccggcactg cgcggtactg acgcacaatt ttaccgttat
    12121 gcgacaggtt gatcgtgacg tcgaaacgcg ccagcgcaat gcggcggatg atttcatcaa
    12181 tatggctaaa ttcggttttc tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga
    12241 acagatccag cacctccagc gtcgtcccca caggatgcgc cgccggtttt accgtcacgt
    12301 tcatatcgcg cccttcggca taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag
    12361 tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc ctcaccgcga aagcccaggc
    12421 tgataatggc ttcgagatcg tccagagagg cgattttact ggtggcatga cgagccagcg
    12481 ccagcgccag ctcatctttt ttgataccgc agccgttatc acgaatgcgg ataagtttcg
    12541 ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca
    12601 ccagttcttt gactaccgac gcaggtcgct cgaccacctc acctgcggca atctggttcg
    12661 ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat
    12721 ctggcgtcgt caccgtactg gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg
    12781 gatgcgcaag gaaataattg cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt
    12841 aatcgtcgct cgccagcaaa cgttcttcgc tgttgttgct gataaaaccg gtttcgacca
    12901 gtactgacgg gatatccggc gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt
    12961 tatgtatttc gccaatgcgt tgcaactgac tgatcatact ggtcgctaca tcatacccta
    13021 cccgttggga atgaccgaac tgtaaatcca gcaccgcctg gcttaaatag gggtcagact
    13081 gactgttcgc cagcacatca cccgccccac ccagcagctc cgactgtttc tcgtgctgct
    13141 ccagccagct ggccatttcg ctgttggcgc gacggttaga gagcacccat acggaagcgc
    13201 cagtcgcact gcggttcggt gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt
    13261 gcttacgtgc cacatcactg cgccccatca ccgagataaa gtaatccccg tcacgggtta
    13321 aaacgccctt aaacatcgga tcgtcattga gcaaagtacg caatttacgc gcgatggcga
    13381 tggtgacatt tttctcccgc gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt
    13441 gtccggcatc aatagcgatg ataattttat cgccagtgtt agccgtcgcg cgcgctgccg
    13501 gacgcgttac cgtattactg ctgataacac ccgtagtgcg gttactttcc gttttaaacg
    13561 gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg
    13621 caaccacggg cggcggagga ggaggtggcg gcgcatcggc gttaatcgta aagacgacag
    13681 tgtaattgct gccattctgc cgcttcaccg cttcggtttt accgttttcg gtaagatcga
    13741 ccaccagccg tagcgtttgt gcatctttag gcgttccaga gcgaatcgcc ttcaccagat
    13801 tattgccgct gaacaacaac ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg
    13861 ccacggtgcg tttgctttga tggctaaacg cataatcagg atcgccaata aaactcaacg
    13921 ttatccgcgc ctgttgatta ccgttagaaa cctgaatatc agagagcgtc gcggcaccca
    13981 ccggcgtgca cagcagcagc agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc
    14041 cgccaccttt caaaggttaa ccggctaaac gcgccagcaa caattcaccc gcagaggaaa
    14101 ccgcactcac gcgcgcctca cggccttgtg cctgataatc aatgtgtatt tcgacatccg
    14161 ggtcaggaag aacacctgta ccttgttgtg gccactccac caggcagatg gcatcgttgg
    14221 caaaataatc gcggatcccc ataaactcca gctcctcggg atcggcaagg cggtacaaat
    14281 caaagtgata aaccattaaa ttgtcgagcg tatagggttc gaccagcgta taagtggggc
    14341 ttttgacatt accctgatga cccagagctt gtaaaaagcc ccggctaaag gtggttttac
    14401 ctgcgcctaa atcgccatac agatagatta cggttgcgcc atcgcaggct ttcgctaccc
    14461 gctcgcccag gtctaatgtt gcctgctcat cagggagcgg aattactcga ttcatcatgg
    14521 tttttatcag tcacttccgg gttaacaata cgctgtagcg tggaaaagag atcggttgcc
    14581 agcatcccgc gcgttccaaa acgcgccgcc agtacgtcag ctgcagcacc gtgcgcgaca
    14641 cagcccgcac aggcggcatc atacggcgac agtttttgcc caagcaatgc gccaataata
    14701 ccagagagca catcgcccat gccgccgctc gccatgcctg catttccggc atcaataatg
    14761 cctaaagcgt caggatgggc ggcgaccacg gttccggcac ctttcagcac cgctacgccg
    14821 ccataacgtt gtaccagacg tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg
    14881 gaacagccta acaaccgtgc ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc
    14941 ttatcgggat tgattgccag caggttcaat gcatcggcat cccacaacat cggtttgcga
    15001 aaattctcaa ctttttgcag tgcttttttc ccccactctt gctggcccag accgggacca
    15061 atcaccacca catcggccca ttccaggctt tcggtaagag agtccatcgt cagttcatgc
    15121 accatcaatt ccggtcgtgc agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact
    15181 cggactaaac cagcaccagc acgtagcgcc gcttcccccg tcatacgaat agcccccgcc
    15241 gtgccgtgat cgccaccgat aattaccagc cgcccgtgat cgcctttatg cgaagtcggg
    15301 cgacgcggtt ttagccagtg agaaagttgt tctgccgaaa accgctgaat tttcgtctcc
    15361 tgacctgcca gccaactatc cagccccagt gagtcaaaat gcagttgtcc ggtaacatcc
    15421 cgcgcttttc cagtgagcaa gcctggtttc agcgcaataa aagtgatggt gtgatcggcg
    15481 ttgatcactg cgcctggcgt agcgccggtt tcagccagca ggccggaagg gatatcaacc
    15541 gccgcaatcg gcgcaggatg ggaattagcg tggtcgatta actggctaat ggattcgcgg
    15601 ggcgcttgct gcaagccggt accgagcagc gcatcaacaa tcagatctac cgattcgggc
    15661 cagacaatat tcgaagcatg gatctcgcct cccgcgttta accatgcttc gcgtgccagc
    15721 gcggcctctt ccggcaacgg tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg
    15781 accgctgtgg ccagtcgcgc gaccacgtag ccatcgccgc cgttattacc atgaccgcac
    15841 agcaccagcc agtggcgggc gtcaggatac gccgaacgac acacctggaa tgcggcttcg
    15901 ccagcgcgaa gcatcagctc atagagtgta agccccagcg catctgccgc ctcacgttct
    15961 ccgcggcgga tatcgtcggc gtgccagacg gtgtgtggta tacttacggg gtttttcttc
    16021 attgtatggt ccgtcatgtc agagcccctc gatctcaatc agttagcgca aaaaattaaa
    16081 cagtgggggc tggaactggg ctttcagcag gtaggtatta ccgataccga tctcagcgag
    16141 tccgagccca aactgcaagc atggctggac aaacaatacc acggcgaaat ggactggatg
    16201 gcacgtcacg gtatgctgcg cgctcgcccc catgagttat tacccggtac gctgcgcgtg
    16261 atcagcgtgc ggatgaatta ccttcctgct aacgccgcat ttgccagcac gctgaaaaac
    16321 cccaaactcg gctatgttag ccgttatgcg ctgggccgtg actatcacaa acttctgcgc
    16381 aaccgactca aaaagctggg cgagatgatt cagcaacatt gtgtttcgct gaattttaga
    16441 ccgtttgtcg attctgcgcc tattctcgag cgcccgttag ctgcaaaagc tgggctcggc
    16501 tggacaggta agcactcact tatcctcaat cgcgaggccg gttctttctt ctttttaggc
    16561 gaattgctgg tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa
    16621 tgcatagcct gtatgacgat ttgcccgacc ggtgccatcg tcgagccata taccgtcgat
    16681 gctcgccgct gtatctctta tctcaccatc gaactggaag gggcgatccc ggaagagttg
    16741 cgaccgttaa tgggaaaccg tatttacggt tgcgatgact gccagcttat ctgcccgtgg
    16801 aatcgctatt cgcaactcac tacagaagac gatttcagcc cgcgtaagcc gctacacgca
    16861 ccggaactca ttgagttatt cgcctggagc gaagagaagt ttttaaaagt cacggaagga
    16921 tcggcgattc gccgtatagg tcacctgcgc tggctgcgta atatcgccgt ggcattaggc
    16981 aatgccccct gggatgaaac gattctggcg gcgctggaga gtcgcaaagg tgagcaccca
    17041 cttcttgatg agcacatagc gtgggcgatg gcgcagcaaa tcgagaggcg aaatgcgtgc
    17101 atagtcgaag tgcaattgcc gaaaaaacag cgtctggttc gggtgattga aaaagggtta
    17161 ccacgtgacg cctgattcat ccacagcttg tgaataaaaa taaaaacgca ttgcaattca
    17221 agacgaataa aatcgtcaag tgatcgactc gacaaaacga aatcttaatt tttaacttat
    17281 ctatcaataa gttaaaaaaa tactattcac cgtgcgaagt ttctttgaaa ttaagactac
    17341 agcctgaggc tgtggataac tctgtttaca agagtttgtc agagacaaca aaaaccagca
    17401 ataacgcatc gcgtcgctgt ggatatttta ttgagagaag a
//
LOCUS       DAZFDP010000104        17269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFDP010000104 DAZFDP010000000
VERSION     DAZFDP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..654
                     /locus_tag="ACHJ1G_003439"
     CDS             70..654
                     /locus_tag="ACHJ1G_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4765236.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            734..1429
                     /locus_tag="ACHJ1G_003440"
     CDS             734..1429
                     /locus_tag="ACHJ1G_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4765237.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1459..3975
                     /locus_tag="ACHJ1G_003441"
     CDS             1459..3975
                     /locus_tag="ACHJ1G_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4765238.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3986..5077
                     /locus_tag="ACHJ1G_003442"
     CDS             3986..5077
                     /locus_tag="ACHJ1G_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4765239.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5120..5980)
                     /gene="rsmI"
                     /locus_tag="ACHJ1G_003443"
     CDS             complement(5120..5980)
                     /gene="rsmI"
                     /locus_tag="ACHJ1G_003443"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4765240.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6044..8080
                     /gene="lpoA"
                     /locus_tag="ACHJ1G_003444"
     CDS             6044..8080
                     /gene="lpoA"
                     /locus_tag="ACHJ1G_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4765241.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8038..8433
                     /locus_tag="ACHJ1G_003445"
     CDS             8038..8433
                     /locus_tag="ACHJ1G_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4765242.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8453..9043
                     /gene="diaA"
                     /locus_tag="ACHJ1G_003446"
     CDS             8453..9043
                     /gene="diaA"
                     /locus_tag="ACHJ1G_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4765243.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9053..9628
                     /gene="dolP"
                     /locus_tag="ACHJ1G_003447"
     CDS             9053..9628
                     /gene="dolP"
                     /locus_tag="ACHJ1G_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4765244.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9742..10782)
                     /locus_tag="ACHJ1G_003448"
     CDS             complement(9742..10782)
                     /locus_tag="ACHJ1G_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4765245.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10855..11490)
                     /locus_tag="ACHJ1G_003449"
     CDS             complement(10855..11490)
                     /locus_tag="ACHJ1G_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4765246.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11618..12136
                     /gene="yhbO"
                     /locus_tag="ACHJ1G_003450"
     CDS             11618..12136
                     /gene="yhbO"
                     /locus_tag="ACHJ1G_003450"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4765247.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12116..12559)
                     /locus_tag="ACHJ1G_003451"
     CDS             complement(12116..12559)
                     /locus_tag="ACHJ1G_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4765248.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12610..12912
                     /gene="yhbQ"
                     /locus_tag="ACHJ1G_003452"
     CDS             12610..12912
                     /gene="yhbQ"
                     /locus_tag="ACHJ1G_003452"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4765249.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12899..13402)
                     /locus_tag="ACHJ1G_003453"
     CDS             complement(12899..13402)
                     /locus_tag="ACHJ1G_003453"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4765250.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13396..13920)
                     /locus_tag="ACHJ1G_003454"
     CDS             complement(13396..13920)
                     /locus_tag="ACHJ1G_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4765251.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14129..15124
                     /locus_tag="ACHJ1G_003455"
     CDS             14129..15124
                     /locus_tag="ACHJ1G_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4765252.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15133..16011
                     /locus_tag="ACHJ1G_003456"
     CDS             15133..16011
                     /locus_tag="ACHJ1G_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4765253.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16217..17224
                     /locus_tag="ACHJ1G_003457"
     CDS             16217..17224
                     /locus_tag="ACHJ1G_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO4765254.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4360 a         4567 c         4362 g         3980 t
ORIGIN      
        1 ctttaaagat gttgaacata tattcacatt aaatatgatt atgtacttgt tacaaggata
       61 aggttataaa tgaataaagt tacaaaaaca gctattgtgg gcttacttgc gcttttcgca
      121 ggtaatgccg ctgcaaccga tggtgaaata gtttttgatg gcgagatttt aaaatccgct
      181 tgtgaaatca atgactctga taagaaaatt gaagttgctc ttggtcacta taatgccgaa
      241 cagtttcgtt ctgttggcga tcgtagccca aaaattccat tcactattcc tttagttaac
      301 tgcccagtga ctgggtggga gcacgataac ggcaacgttg aagcgtcttt ccgtttatgg
      361 ctggaaacgc gggataatgg caccgtccct aacttcccta atctggcaaa agtgggctct
      421 tttgctggca cagcggcaac aggtgtgggt atccgtattg acgatgcgga aagcggaaac
      481 cttatgccac tgaatgctat ggggaatgat aacacggttt atcagatccc agcggactcc
      541 aatggtattg tcaatgttga cctcatcgct tactacgtat caaccgttga agcatcagaa
      601 atcaccccag gggaagcaga cgctgtcgtt aacgtaacgc tggattaccg ttaatacgtt
      661 acggcgttat ctgacctttc agataacgcc cttttccttc ctctttctcg ttgtatcagg
      721 ttgaaaaata actatgtcaa aacgaacatt cgcggtgata ataaccttgt tgtgtagctt
      781 ctgtatcggc caggcgcttg cagggggtat cgttttacag cgaacgcgag tgatctatga
      841 tgccagccgc aaagaggctg cgttacctgt cgcaaacaaa ggcgcagaaa cgccttattt
      901 actgcaatca tgggtagata atatagatgg tacaagccgt gccccattta ttataacccc
      961 accgctattt cgtcttgaag ccggcgatga ctcatcactg cgaattatta aaacagctga
     1021 taacctgcct gaaaataaag agtcgctgtt ctacattaat gttcgtgcca ttccagcaaa
     1081 gaaaaaatca gatgatgtta atgctaacga gttgacgctg gtatttaaaa cacggatcaa
     1141 aatgttttat cgccccgcac acctgaaggg acgggtaaac gatgcgtgga catcactgga
     1201 atttaaacgt agtgaccatt cactcaatat atataaccca actgaatatt acgtcgtatt
     1261 tgccggactg gcagtcgata aaaccgatct cacaagcaaa attgaatata tcgcgcccgg
     1321 agaacataaa cagttaccac ttcctgcatc tggcggaaag aacgtgaaat gggctgcaat
     1381 caatgattat ggcggcagtt ccgggacaga aactcgtcca ctgcaataac aaatataaaa
     1441 aacacaggtc atcagggaat gccacaacga caccaccagg gacataaacg cacaccgaaa
     1501 cagttggcgc tcatcatcaa acgctgtttg ccgatggtgc tcactggcag cggcatgctt
     1561 tgcactaccg ctaacgccga agagtattat ttcgacccca ttatgctgga aactacaaaa
     1621 agtggtatgc aaacaactga tctgtcacgt ttttcaaaaa aatacgcaca actaccagga
     1681 acttatcagg ttgatatctg gctgaataaa aagaaggttt cacagaaaaa aattacattt
     1741 accgccaatg cagagcaact tctgcagcca cagtttacgg tagaacaact acgtgagctg
     1801 ggtattaagg tggatgaaat cccggcgctg gctgaaaaag atgacgatag cgtgatcaac
     1861 tcgcttgaac aaatcattcc cggtacagct gctgaatttg atttcaatca tcagcgactt
     1921 aatttgagca ttccccaaat tgcactgtac cgtgatgcaa gaggttacgt ctccccttct
     1981 cgttgggacg atggtatacc aacgctgttt accaactact cgtttacagg ttctgataac
     2041 cgttaccgcc agggcaatcg tagccaacga cagtacctga atatgcaaaa tggtgctaat
     2101 tttggcccct ggcgattacg taactattct acgtggacac gcaacgatca ggcgtcaagc
     2161 tggaacacta tcagtagtta tttacaacgt gatatcaagg cgttgaagtc tcagttgctt
     2221 ctgggagaaa gcgccaccag cggcagtatt ttttccagct acacctttac tggcgtgcaa
     2281 ctcgcttccg acgataatat gttgccaaac agccagcgcg gatttgcccc aacggtacgc
     2341 ggtatcgcaa acagtagtgc aatcgtgact atcaggcaaa atggttatgt gatctatcaa
     2401 agcaacgtgc cagcgggtgc ctttgaaatt aacgatctct acccctcttc caacagcggc
     2461 gatttagaag tcacgattga agaaagtgac ggtacgcaac gtcgctttat ccagccttat
     2521 tcttcattac ccatgatgca gcgacctggg catctaaaat atagcgcgac cgctggacgc
     2581 tatcgcgctg atgcaaacag tgatagcaag gaacccgaat ttgctgaagc cacggcaata
     2641 tatggtttga ataatacttt tacgctgtat ggcggcctgc tcggttctga agattattat
     2701 gcgctgggga tcggtatcgg cggcacactt ggcgcactgg gcgcgttgtc gatggatatc
     2761 aacagagctg acacccaatt cgataaccgg cactcttttc atggctatca atggcgtacg
     2821 cagtacatca aagatatccc ggaaaccaac accaatatcg ctgttagcta ctatcgctat
     2881 accaacgatg gctattttag ttttgatgaa gccaataccc gtaattggga ctataacagt
     2941 cgccaaaaaa gtgaaattca attcaacatc agccagacaa tatttgatgg ggtaagtctg
     3001 tatgcctccg gttcgcagca agactattgg ggcaataacg ataaaaacag gaatatctca
     3061 gttggggttt ccggtcagca atggggaatt ggttacagcc tgaattatca atacagtcgc
     3121 tacacggatc aaaataatga ccgcgcactc tctttgaatc tcagtattcc gttagaacgc
     3181 tggttaccgc gtagccgggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
     3241 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
     3301 caaaatctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
     3361 tatggcacct tcagtgccgg atacagctac ggtaatgaca gcagccaata caattacggc
     3421 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
     3481 gcttttgcgc ttatcgatgc taatggggct tctggcgtga ggatacaaaa ctatccgggg
     3541 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta ccaggaaaac
     3601 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgagca aacaacacag
     3661 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
     3721 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
     3781 agcaacgatg agacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
     3841 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
     3901 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcacg
     3961 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
     4021 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagctgaca gcactagcgg
     4081 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
     4141 aggacgggag tttacctctg ccacgctaag tagcactaac tggcaatacg cctgtacctg
     4201 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cctgtactta ccaccactgg
     4261 acatcaagca ggatattaca aactcaatga tagtctggat attaaaacca cattacaggc
     4321 gaatgacatt ccaggactcg tgactgacca gaccgtttct gttaacaccc gattcacaca
     4381 gataaaaagc aacaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     4441 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     4501 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcatg
     4561 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     4621 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaggt
     4681 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     4741 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     4801 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     4861 acgaagaagt tcagacaatg cgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     4921 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     4981 catcaacatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     5041 tcagggcacc gccaccatta ccgtcatcgt gcggtaaaag aagacgttgc gttttgatta
     5101 acgcaacgct tcggcactct tacccctgct gctccagcgc atacttatac agcgcatttt
     5161 tcttcacgcc gtgaatttct gccgccagcg ccgccgcttt tttcaacggc agttctgcct
     5221 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     5281 cttcgataat cagcaccatt tcgcctttgc gacggttttc atcttctttt acccacgcca
     5341 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     5401 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     5461 ggtgggtgga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     5521 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagagggta
     5581 aacccgcggc gcttaacgca gtgatagcag cacacggccc gggtagcggc accacgcaaa
     5641 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     5701 cggcatcgga aaccagcgca atgttctgcc cttcttgcag cttcgccagc agcgtttcgg
     5761 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     5821 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     5881 cctctaacgc acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     5941 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     6001 tttaatattg agcattgcgt aaaaaaatat cactggatac attatggtac cctcaacatt
     6061 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     6121 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     6181 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     6241 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     6301 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggt
     6361 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     6421 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     6481 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgctcgg
     6541 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     6601 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     6661 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     6721 cgactggcaa aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     6781 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     6841 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagcgg cgaaaaatat
     6901 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagctgaaca
     6961 acctcagccg caaaccgtgg atggcgttgc cagcccggca caagcctcgg ttagcgatct
     7021 gaccggtgaa cagcctgcgg cacagccagc gcctgtaagc accccggcag caaccaccac
     7081 agcggtaagc gcgccagcga atccatccgc agaactgaaa atctacgata cctcatcaca
     7141 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     7201 gttgctgaaa aataacgttg aagagttgct taagagcaac accccgctga acgtgctggc
     7261 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     7321 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     7381 ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc aatgcgtttg cgcaggagtg
     7441 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     7501 cgcgggtgtt aacggcggtt ctggtatcgc tttaacgggt accccgatta ctcccagagc
     7561 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     7621 ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga
     7681 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     7741 gtacgccagc tcccgcagtg cgcaagggac cgctggcccg gatttccgac tggagatgga
     7801 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctaccgt taatgcagca
     7861 ggcactcagc gcggtgaata acgattattc actggctcgc atgtatgcga tgggcgtcga
     7921 tgcctggtcg ctggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     7981 cggtaatacc ggaagcctga cggcaaaccc ggattgcgtg attaacagga agttatcatg
     8041 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     8101 gcgtgggaag tacaagcgcg tcgctggctg gaaggcaaag gactgcgatt tgtcgccgct
     8161 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggccg gaccaccgtt
     8221 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     8281 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     8341 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     8401 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     8461 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     8521 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     8581 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     8641 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     8701 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     8761 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     8821 cgatattgta aaagcggtag aagctgccgt tacgcgtgat atgactattg tggcattgac
     8881 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caagatgtgg agatccgcat
     8941 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     9001 cgatctgatc gataacacgc ttttccctca ccaggatgtt taaggagaat acatgaaggc
     9061 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     9121 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     9181 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     9241 gattaagaaa gaagcccgta ttaatgtcac ggcgtatcag ggcaaagtgc tgctggttgg
     9301 gcagtcacca aacgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     9361 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcgtc
     9421 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     9481 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     9541 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     9601 aaccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     9661 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     9721 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     9781 cactgccacc atcgcccctg tcagccataa ggctttcgct gggaacgctt tgcgcagcat
     9841 aatcagtgac ggcaaactca ccgccgggag cgtcatcaac aatgccagcg ccggagcagt
     9901 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     9961 aaacaagcat cctgctaccg ccatcgccat cacccacatc aggctgttat cgacagcacc
    10021 atcggcatgg gggaataacc agacgcgagc ggcacccaac accagtactg caaggatgta
    10081 aaccgggatc gtactccaga aaagcgtcca tagcgccctg ccccagcggc taaaaaaacc
    10141 gccctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
    10201 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
    10261 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
    10321 cggattgccc atccagaatg ccagcgcacc gcccatcgac acctgttggc gacgcattcc
    10381 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
    10441 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
    10501 cagcacctga atcaacgaac cgaggatcac ccccagcact gccgctttcc ataccgcgag
    10561 gaaatagagc atcgcgtaat ccaacgccgc ctgccacgga ttagcatccg cctgcgcaag
    10621 gatagattta ccgatactgt gggtttcggc agcagtaaac gctttgccgt agtaaggttg
    10681 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
    10741 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
    10801 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
    10861 agctcttttg cgtaattctg aagagcttaa aatcgtcaca ccttcatgcc cgggccgcat
    10921 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttaccggg
    10981 taataaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
    11041 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcga ttaatgcttc
    11101 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
    11161 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
    11221 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
    11281 gagacaacaa aacacaatgt cgatgggatc ggtgacctgc gccagcgcgt cagtcagttg
    11341 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcggc
    11401 tatggcgtta actttcggtt cgttaatcaa catccgcagc aggtgaccgc ccaccagccc
    11461 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
    11521 cgctccacgg ctcatgcacc aggcttaata gaccatgagg taattatccc cgattgtggg
    11581 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
    11641 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
    11701 cggacacgaa gtgattacca ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
    11761 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
    11821 cctgctgcta ccgggcggcc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
    11881 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
    11941 gctgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcggtta aaccgatcat
    12001 tattgatgtt aaaaacgcgg gcgcggaatt ttacgatcag gaagtcgtgg tcgataaaga
    12061 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
    12121 cctgctcggt gcctgagccg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
    12181 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
    12241 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
    12301 ggatctcacc tttaaactgc acgccgcgaa ttaacgctac cgttttcggc tgaccgttta
    12361 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
    12421 tcaaaatgta gaaggcgact ttctgcgcat caaacagata aaaagcatta gcgcaccaga
    12481 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccaac caacggctga
    12541 tggtgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
    12601 acgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
    12661 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
    12721 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgatcgt
    12781 tcgctggcgt tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
    12841 ctggtagcgg aaggcgcagg gtttgcagag ctgttaagca gtctgcaaac cccggagatt
    12901 aaaagcgatt gaaatgctcg tgatactcaa ccagaccagt tacgccattc aacgcgtcat
    12961 ccgctaaacg atgtacctgg aaggcgcttt cagtgcctgg ccagcggcaa cgcaggtcgt
    13021 gatgtgccgc cagttcaaag ccaaaacggc tgtacagcgc cggatcgccc agcgtcacca
    13081 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
    13141 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    13201 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    13261 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    13321 cttccgcatc actttcgaat gagcgacgta gcagggcatc aatacccggc gcatcaatgg
    13381 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    13441 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    13501 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacagcccca gctccgtatc
    13561 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    13621 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg cgttctggct
    13681 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    13741 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    13801 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    13861 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    13921 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    13981 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    14041 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    14101 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    14161 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    14221 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    14281 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    14341 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    14401 gctgatcctc gccgacctcg ccatgctaga gtacgccgcc gagcgttacc cgcatattga
    14461 gcgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    14521 ccattttgac gttgctcgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    14581 actggcacgg gtcacacctg taccactgga agtctttgct ttcggcagcc tgtgcattat
    14641 gtcggaaggt cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acaccgtagg
    14701 cgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    14761 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    14821 atgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    14881 tctcaatacc ctggaactgc tgccggagtt aatggcggcg aatattgctt cggtgaaaat
    14941 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    15001 tatcgaccgt tgtaaggccg atccgcaaaa cttcataccg caaagcgcgt ggatggagac
    15061 gctcgggtcg atgtccgaag gcacgcagac cacccttggc gcgtatcacc gtaaatggca
    15121 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    15181 ctggaagatt tttatcagca ggccgccacc agcagcgccg acgtgattta tcttggtgaa
    15241 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact ggctggagat ggcaaaatcg
    15301 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    15361 gaactgggcg aactgaaacg ctatgttgag aacggtgagt ttctgattga agccagcgat
    15421 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    15481 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    15541 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    15601 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    15661 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    15721 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    15781 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    15841 atgcaggggc tggttgatgt ggtccgcctg tcaccgcagg gtactgacac tttcgcgatg
    15901 ctcgacgcct tccgcgctaa tgaaaatggc gctgcgccac taccgttgac ggcaaacagc
    15961 gactgtaacg gctactggcg gcggctggcc gggctggagc tgcaagccta agtaaatatg
    16021 ctcgtcatac ttcaagttgc atgtgctgca tctgcattcg ttcaccccag tcacttactt
    16081 atgtaagctc ctggggattc actctcttgt cgccttcctg caactcgaat tatttagagc
    16141 acagctcact ttgttaacaa ctttaactac tctttaatgc agtattaaag attaatcggt
    16201 aacaaagtga gctgttatga ctgataaaac cattgcgttt tcgctactcg atctggcccc
    16261 cattcccgaa ggttcttcag cgcgagaagc attctcccac tctctcgatc tcgcccgtct
    16321 ggctgaaaag cgcggctatc atcgctactg gctggcagaa caccacaata tgactggcat
    16381 tgccagtgct gccacgtcgg tattgattgg ctatctggcg gcgaatacca ccacgctgca
    16441 tctggggtct ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg ccgaacagtt
    16501 cggcacgctc aatacactct atccggggcg aatcgatttg gggctgggtc gtgcgccagg
    16561 tagtgaccaa cggacaatga tggcgctgcg tcgccatatg agcggcgata ttgataattt
    16621 cccccgcgat gtcgcggagc tggtggactg gtttgacgcc cgcgatccca atccgcatgt
    16681 gcgcccggta ccaggctatg gcgagaaaat ccccgtgtgg ttgttaggct ccagccttta
    16741 cagcgcgcaa ctggcggcgc agcttggtct gccgttcgcg tttgcctcac acttcgcgcc
    16801 ggatatgttg ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt cggcacgact
    16861 ggaaaaacca tacgcgatgg tgtgcatcaa tattatcgcc gccgatagca accgcgacgc
    16921 tgagtttctg tttacctcaa tgcagcaagc ctttgtgaag ttgcgccgtg gcgaaaccgg
    16981 gcaactgccg ccaccgattc aaaatatgga tcagttctgg tcgccgtccg aacagtatgg
    17041 tgtgcagcag gcgctgagta tgtcgttggt aggtgataaa gcgaaagtgc gtcatggctt
    17101 gcagtcgatc ctgcgcgaaa ccgacgccga tgagattatg gtcaacgggc agattttcga
    17161 ccaccaggcg cggctgcatt cgtttgagct ggcgatggat gttaaggaag agttgttggg
    17221 atagtgtgtc ttaacgcggg aagccttatc cgagctggca acgctgtcc
//
LOCUS       DAZFDP010000105        17159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFDP010000105 DAZFDP010000000
VERSION     DAZFDP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..756
                     /gene="ais"
                     /locus_tag="ACHJ1G_003458"
     CDS             154..756
                     /gene="ais"
                     /locus_tag="ACHJ1G_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4765255.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(795..1220)
                     /gene="nudI"
                     /locus_tag="ACHJ1G_003459"
     CDS             complement(795..1220)
                     /gene="nudI"
                     /locus_tag="ACHJ1G_003459"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4765256.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1499..2041
                     /gene="yfaZ"
                     /locus_tag="ACHJ1G_003460"
     CDS             1499..2041
                     /gene="yfaZ"
                     /locus_tag="ACHJ1G_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4765257.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2141..3343
                     /gene="yfaY"
                     /locus_tag="ACHJ1G_003461"
     CDS             2141..3343
                     /gene="yfaY"
                     /locus_tag="ACHJ1G_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4765258.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3407..3868
                     /locus_tag="ACHJ1G_003462"
                     /pseudo
     CDS             3407..3868
                     /locus_tag="ACHJ1G_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3909..4811)
                     /gene="rpnE"
                     /locus_tag="ACHJ1G_003463"
     CDS             complement(3909..4811)
                     /gene="rpnE"
                     /locus_tag="ACHJ1G_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4765259.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5005..6195)
                     /gene="glpC"
                     /locus_tag="ACHJ1G_003464"
     CDS             complement(5005..6195)
                     /gene="glpC"
                     /locus_tag="ACHJ1G_003464"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4765260.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6192..7451)
                     /gene="glpB"
                     /locus_tag="ACHJ1G_003465"
     CDS             complement(6192..7451)
                     /gene="glpB"
                     /locus_tag="ACHJ1G_003465"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4765261.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7441..9069)
                     /gene="glpA"
                     /locus_tag="ACHJ1G_003466"
     CDS             complement(7441..9069)
                     /gene="glpA"
                     /locus_tag="ACHJ1G_003466"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4765262.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9342..10700
                     /gene="glpT"
                     /locus_tag="ACHJ1G_003467"
     CDS             9342..10700
                     /gene="glpT"
                     /locus_tag="ACHJ1G_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4765263.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10705..11781
                     /gene="glpQ"
                     /locus_tag="ACHJ1G_003468"
     CDS             10705..11781
                     /gene="glpQ"
                     /locus_tag="ACHJ1G_003468"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4765264.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11823..12062)
                     /locus_tag="ACHJ1G_003469"
                     /pseudo
     CDS             complement(11823..12062)
                     /locus_tag="ACHJ1G_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12060..12164
                     /locus_tag="ACHJ1G_003470"
                     /pseudo
     CDS             12060..12164
                     /locus_tag="ACHJ1G_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12244..12894
                     /gene="inaA"
                     /locus_tag="ACHJ1G_003471"
     CDS             12244..12894
                     /gene="inaA"
                     /locus_tag="ACHJ1G_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4765265.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12948..13202)
                     /gene="yfaE"
                     /locus_tag="ACHJ1G_003472"
     CDS             complement(12948..13202)
                     /gene="yfaE"
                     /locus_tag="ACHJ1G_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4765266.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13202..14332)
                     /gene="nrdB"
                     /locus_tag="ACHJ1G_003473"
     CDS             complement(13202..14332)
                     /gene="nrdB"
                     /locus_tag="ACHJ1G_003473"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4765267.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14421..16706)
                     /gene="nrdA"
                     /locus_tag="ACHJ1G_003474"
     CDS             complement(14421..16706)
                     /gene="nrdA"
                     /locus_tag="ACHJ1G_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4765268.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRALGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16834..16977
                     /gene="ypaB"
                     /locus_tag="ACHJ1G_003475"
     CDS             16834..16977
                     /gene="ypaB"
                     /locus_tag="ACHJ1G_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4765269.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4048 a         4189 c         4586 g         4336 t
ORIGIN      
        1 tttcttattt aggctttgca tttggcaaaa ttttgaggca ttttgccgac atcgtaggat
       61 ttttaatatt acaccaactg ctaattatcg ccagaaatgt cgctcaattt tacggtaatt
      121 gtctggttgc gcttgtctat aggtggagtt tacgtgttag ctttttgccg ctcatcattg
      181 aagtcaaaaa aatatttcat cattttactg gcgctcgctg caattgccgg actgggtact
      241 catgccgcct ggagtagcaa tggtttgcca cgtatcgaca ataaaacatt ggccagactg
      301 gcacagcagc acccggttgt cgttttgttt cgccatgctg aacgttgcga tcgttcaacc
      361 aatcaatgct tgtcagataa aacaggtatt acggttaaag gtacccagga tgcccgtgaa
      421 ctgggcaacg cttttagcgc tgacatccct gatttcgatc tttattccag taataccgtc
      481 cggaccattc agtcggcgac ctggttttcc gcgggtaaaa agctgacggt agataaacga
      541 cttcttcagt gcggtaatga gatttatagt gcaattaagg acttacaaag caaagcgcct
      601 gataaaaata tcgttatttt cacccataat cattgcctga catatattgc taaaaataag
      661 cgtgatgcga catttaaacc tgattatctg gatggtttag tcatgcatgt ggaaaaaggc
      721 aaagtttatc tggatgggga attcgttaac cactaaattt tcaatctgac agccagtgat
      781 ggctgtcatc attgttaaag aagacctttc aaacgtaacg tttttcgggt ggcaacattt
      841 aaatcataat gcaccagatc ttcaggtttt acccacgcgt agtcctgaaa ctcttcgttt
      901 attttcactt ctcggttggc agaaacgcag tcaaaaatca ggtaaatcat ataaatctct
      961 tccttgcgac catctgcata cgtcttggtg cgaatatcat cgctgaaggt ccacggcgtg
     1021 atttctgtca aaagcagctg ttctcccagt tcttcgcgaa tttcgcggcg tagtgcctct
     1081 tcaattcgtt cgccagactc cacgccgcca cccgaaagcg cccattgacc ggggaaaacg
     1141 ccgcgatcgt cggccatttt acacagcaaa taagcaccat cattttgaat caaagggcat
     1201 acaatagtcc gttgtcgcat aggggtattc cttataaata aaaagaaacc atgagaatac
     1261 ggtaactcag aaatatgtga aagtgacagt aatctctttt gcaatgataa ctgcacttat
     1321 tattattttt gtgtgtatcg gtaaagtgtg tttatttttt cggcgtttaa ttatttaaga
     1381 agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc ctctgtgcca
     1441 ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa aggggataat
     1501 gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat cggtcaatgc
     1561 aatgagcatc agcggccagg cgggtaaaga atacaccaat attggtgtcg gttttgggac
     1621 tgaatcgacg ggcctggctt taagcggtaa ctggacacat aacgacgacg acggtgacgt
     1681 cgcgggcgtg gggctggggt tgaatctgcc tctcgggccg ttaatggcga ccgttggcgg
     1741 aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt tatgccgcag cggtaggagg
     1801 tggtttgcag tggaaaattg gcaacagctt ccgtttgttt ggcgagtatt actactctcc
     1861 ggattcgctc tccagcggta ttcaaagtta tgaggaagcg aatgctggcg cgcgttacac
     1921 cattatgcgt ccagtcagta ttgaggcggg ttatcgctac ctgaatctgt cgggtaaaga
     1981 cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt ggggttaacg ccagtttctg
     2041 atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc ttactacgcc ttatctccct
     2101 ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag tggaaatgct
     2161 atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg cctggctggc
     2221 cgattttttc tttcatcagg ggctgccatt atctcgccgc aatacggtgg gggataacct
     2281 tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgc tgatcgttaa
     2341 cggcgggctg ggaccgacca gcgatgattt aagcgcactc gccgctgcga cagcaaaagg
     2401 tgaaggcatg gtgctgcatg aagcctggct caaagagatg gaacgctatt tccacgaacg
     2461 tggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca gtgctgaatt
     2521 tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg cagcttaatc gttgcctgat
     2581 gttctttact cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg aaatcctgcc
     2641 gcgtctgcgc gagcgttttt ctttaccaca gccgccggtt tgtctgcgtt taactacctt
     2701 tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc cgccgggcgt
     2761 aacaatgggc tatcgctctt caatgccgat tatcgaactg aaactcaccg gaccggcaag
     2821 cgagcaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg gacagagcgt
     2881 gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc aaaaccgcca
     2941 gttcagcctg acgttgagcg agcaattcac cggtggttta ttggctttgc aactttctcg
     3001 cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca caggaagaaa ccctggcgca
     3061 aactgcgcac tggattacag aacggcgggc caaccatttt gccgggctgg cactggctgt
     3121 ttcgggtttc gagaacgaac atctcaactt tgcgctagcc acaccagacg gcactttcgc
     3181 tctgcgtgtg cgtttcagca ctacgcgcta cagcctggct atccgtcagg aagtgtgcgc
     3241 aatgatggca ctgaatatgc tgcgccgttg gttaaacggc caggatatcg ccagtgagca
     3301 tggctggatt gaggttattg agtccatgac cttatctgtc tgattccgcc actctgtgcc
     3361 ggtttactgc accggcacat tccccctccg gcaaaacttg tccaaccctc cctacggtga
     3421 gcgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg ggacgcattg agaattacat
     3481 ggcgcaagtc aacgattcgc tttgtctgtt agtgcaggtg gaaagtaaaa cggcactgga
     3541 taacctggac gagatcctcg acgtcgaagg gattgatggc gtgtttattg gaccagcgga
     3601 tctttctgcg tcgttgggct acccggataa cgccgggcac ccggaagtgc agcgaattat
     3661 tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg gctggttttc tggctgtggc
     3721 tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga
     3781 cacgatgctc tacagcgatg ccctggatca acgactggcg atgtttaaat caggcaaaaa
     3841 tgggccacgc ataaaaggta gttattgata tcaaaggccc atggggatcg gctgtgggcc
     3901 tgtgttaatt agtggttatt cgctgccaga tcggcttcgc ttagctgggt ggccgcgagc
     3961 acctggtcac gatcaatgcc ctgttccagc atgcgcaaag caattttaat ggcttgttca
     4021 tgcatacctt cctgccagcc aatttgatgc ccttcctgcc gtagccgttc agcaatagtc
     4081 attaatctct ccttttgtag tggtgaacgt tcggcaatct cctcaataaa acgggtgaaa
     4141 cgggaggtat cgccgcattg cagcagataa ttaaacagtg tttgtagctg gctgtcatta
     4201 gtgaagcctc taaccaaaag cgtggtgatc ctgtcgacca tgccgattaa atcgcggtcg
     4261 cgaatatgct tttgaatcag ttccagcaga gctatacgcc gatgttgcat gatctcatcg
     4321 tcaggtacga tggtgatatc caccaacgga aaagcttcgg tgtacaactg ccgggcaagt
     4381 tgcggatcgt caaactcatc cagccagtta agtgagtacg ggtagggcga tgtttcgcca
     4441 tgataaaaca gcaatggcac caccagcgga actctgtcat agcctttatc caggtgacgc
     4501 tgcatggcgg cagtggcata gcgcattagc cgaaaagcca tattcttttc tgcagagctt
     4561 tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc tggtttccac ggaccacaaa
     4621 acatccgagt agtaagcgcg taaacttttt tcaataaaac tagtgggttc caggcgtaag
     4681 gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat gtatttcgag aaaatcccgt
     4741 gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat catgcgggga ggatgttgtt
     4801 gattcggtca tggttttccg tcacctgata tttatgatga cgcgacatta cgtctatccc
     4861 gggcctttga catctgtcag tttcagcctt tgcgagacat tttcagagga attttcaaag
     4921 cgttgcaacg catacatggt gcgtggaatc gcgctcgcaa aaatgcccga taagcaatgt
     4981 gcttaccggg catcaaaagg tcggttaagc cagcgcctgg gccagtagcg taatcggatg
     5041 ttcgcagcga aggctcgtgg acatctcaat ctgccatttg caggtttcgc agtcggtgat
     5101 gaccagatcc gcgccgctct cttctatctg gcggaacagt ggcgcaccga tggcttgtga
     5161 ggtggggtag ttctcttttt tgaaaccgta agtacccgca ataccgcagc actgggaatc
     5221 cagcaccgtt aactcaagcc ccgggatttt acgcaacagc tccagggtgt agagcgtcca
     5281 gcccattttt tccatatggc acggagtgtg ataaaccact ttcagcggca gcggtttcag
     5341 cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag cgggttgcca gttcgatatg
     5401 atcgcgcaag cctttgttgt cgacattcag cacttccggg tattcgtcgc gcagggcaaa
     5461 tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt actcccacag cttcgcggat
     5521 cgactctaca ttcgtaattg cctgtttgcg tgctttatcg gtaaagccgt tggcgattag
     5581 cggtacgccg cagcattttt ctttgctgag cagttgtaca ccggtaccca ttgcgttgag
     5641 cactttaatt aaatctttac ctaactgcgg atggttgtag ttaacgaagc agccgtgaaa
     5701 gaaagcgacc tggtctttat attgtgcttg ctgagccgct atgctgcgat accagcgacg
     5761 aaacgtaccg aaggagtatt tcggtagcgt gcggcgatga tcgattttta acgccgcatc
     5821 aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg acgattggtg cgaacggcgt
     5881 ggaaacgcta cccatcaggt cggtatgact caacacaaaa ttacgcagcg acgggcgcgt
     5941 ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca atcttcacat cggacgggca
     6001 ggcgacttca caacgtttgc agttgatgca atatttcagc gcctcgtcat acagtgcgcc
     6061 atctttcaaa cgcagacgct cgccatccgg cccggcttgt tttggccctg gataaccggg
     6121 attcacccgg ctcaccgggc aggcggtggt gcagacggtg cacttaatgc agttttcgaa
     6181 gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa tctgttgagc ggcatgtaaa
     6241 gcactgacgg cacaaacacc gccgccgcat ccctgggcga tgggatcaaa tccgcccagc
     6301 accgaaccga tggcaaacag gttctcaatg gtttgccctg cctgcgacgg gcgtagcgtc
     6361 tgatcagtgg ttacaccgaa ctgctgccac ggttgcggcg caaaaaaatc gcccttatac
     6421 cattcacccc gcgtggcggt ttgtagcaca tcaaggccga gaatcggctc gcgaatgccg
     6481 ttacgttccg ctaccagtcc gccactaaag aaactgccgc tggcgagaac cgcgaaacgt
     6541 ggacgtagcg gaatatcggc gtgattgcgg gtccagattt cattcactac gccattttta
     6601 caggtcactt ttttcacttc atcgcccggc atccacacgc caccctggcg cacaaactgg
     6661 cgctgtaact ggttttgcag acgaatgccc agcacggaag gcggcagcgt tggcaaaagc
     6721 atcagtgaac aaggtagttt ttcattcaac caacgccaca gtttgtcatc ggccaggccg
     6781 aagcaggcgg gcatcaggat catttcgcag gtattggcga caggaataag cgcatcaagt
     6841 aacagcggcc agttttcttc attatcaagg aaacgggcga tattcaccgc gcgaaattcg
     6901 gtggcgttat tgcgcaggac atccagttcc ggcagctcta tttctgcggt ttcaaccgaa
     6961 agatccagtt cgcgcaacga agccgccgca agatgcgcct ggaaatccat cagcccgctt
     7021 atccccacta cacatatctt cttcgcgggt agcggccaga cgggaacttc tggcgaactt
     7081 aaccaggttg cacgcaaagt gccgagcggg gtaacgcgct gatgggccag ttccacgctg
     7141 ccctgcaatt gcgcaccgct ttcagcgatc aatgcctgcg cctggcaagc gagatcgagc
     7201 acgcgttgtg gttcgagaag actgtaagga tgggctggtg cctgctgacg caaagattcc
     7261 agtccactgt gaatgtctgt caccggttga ccatcaggca gatggctcag caaatccagc
     7321 gaaccggatg agaaatgcag cgcgctttga ccacgcgtga caatggcaca gcgcaggccg
     7381 tgtttttgca gttgcaggcc acagagtaat ccggcgaggc cgccgcccat aatgacagta
     7441 tcaaagcgca tctttctgct ccttctccag accacacaat ccctgataaa cccagcgggt
     7501 aaattcgctt tcgcgcagtg catctcccca ggcgatgggt tgcacgcctt tccagcgttc
     7561 gttaaggaag gtggaaagtt gctcgataga ttgcgcggac gtcgtgacgt taaaacgttg
     7621 cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg caggtaccca tccccacacg
     7681 ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa ttttctaccg cgtactgaac
     7741 ttcacccgca gttaccgctt cgcactcaca taccaggcta cgatgcagac ggccttcgct
     7801 cagccaggca ggcgtgcgat cgccatgacg ataaaccgca gaaccgcgca gcggggcagg
     7861 cagggagatg actttacgca aggtaacttc agcgggttct tgtgaaccag gcagtgccag
     7921 atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg cataccgcgt cggtagccca
     7981 ttcagccatc agccgatagg tcatcagttt gccaccggtg atggtgataa atccgtccag
     8041 accatcgcgt tcagcatggt cgagcagcac gatgccacgg ctgacgttac gtccgctcgg
     8101 gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag gcccgcaaaa tgcgcgtttt
     8161 cgccatcacg ggggccagtt tttccccttc acgcagcaga atatcaacct cttctgccgt
     8221 cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa gaggtggtgc caatcagcga
     8281 aatggtatcg ccaggcacca gaatatcggc gtcggaaggt ttacggcagc ggttgatcac
     8341 atgctggtta atgcggtgat ccatgatcag cagcgatcct ttcgccggga acatgcgaat
     8401 gcgcagatcg gcatattcgg caatgtgttg cccccagatc ccagcggcat taaccacgac
     8461 aggtgcatga agggcctgag tttcgccggt gagatggtta cgtacacgaa caccgcacac
     8521 cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg gtaaggataa cggcaccgtg
     8581 ttctttggca tccagcatgt ttgctgcggt cagacgaaat ggatcaacgg tgccatccgg
     8641 aactttcacc gcgccaatca gtgccgggtt aacggcaggt tcgataatgc gcgcttgctg
     8701 cgggtctata gcttctgcgc tgatccctgc ttcttcgcag gcgcgaataa aagtggcctg
     8761 gaatgagagg tcatcttccg gcagggtgat aaacaggccg ttggttggtt caacgcagtg
     8821 acgtgcaatg cgtttcagga tctggttttc actaatgcat tcgcgggccg attccgcatc
     8881 ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg ttacgcccgg tggcaccggt
     8941 tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg cgcagggcac agtcgcgggc
     9001 aatcccggct cccgttgcgc cgccaccaat gataatcacg tcacttgatt gcgagtcgcg
     9061 agttttcatt gttatccctc tgaagttcgt tttttaccat ttagccatag taaaaacatg
     9121 aattgtttga tttcgcgcat attcgctcat aattcgaaag tgaaacgtga tttcatgcgt
     9181 cattctgaac attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaatttc
     9241 tgaatgtttt cttaacatcg cggcaactaa agaaacggca ggttctccca ctgaatcagt
     9301 ctgttaatca taaataagac cacgggccac ggaggctttc aatgttgagt atttttaaac
     9361 cagcgccaca caaagcgcgc ttacctgctg cggagatcga tccgacttat cgtcgattgc
     9421 gctggcaaat tttcctgggg atattctttg gctatgcggc ttactatttg gttcgtaaga
     9481 actttgcgct tgctatgcct tatctggtcg agcagggatt ctctcgcggt gatttaggtt
     9541 tcgcgctttc ggggatctcg atcgcttatg gattttcgaa attcataatg ggttcggtat
     9601 cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt gattctggcg gcggcagtga
     9661 tgttgtttat gggctttgtg ccatgggcga cgtcgagcat tgcggtgatg tttgtactgt
     9721 tgttcctctg cggttggttc caggggatgg ggtggccgcc gtgtggtcgt actatggtgc
     9781 actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt gtggaactgt gcgcacaacg
     9841 tcggtggtgg tattccgccg ctgctgttcc tgctggggat ggcctggttc aatgactggc
     9901 atgcggcgct ctatatgcct gctttctgcg ccattctggt ggcattattc gcctttgcga
     9961 tgatgcgcga taccccgcaa tcctgtggct tgccgccgat cgaagagtac aaaaatgatt
    10021 atccggacga ctataacgaa aaagcggaac aggagctgac cgcgaagcaa atcttcatgc
    10081 agtacgtgct gccgaacaaa ctgctgtggt atatcgccat cgccaacgtg ttcgtttatc
    10141 tgctgcgtta cggcatcctc gactggtcac cgacttatct gaaagaggtt aagcatttcg
    10201 cgctggataa atcctcctgg gcctacttcc tttatgaata tgcaggtatt ccgggcactc
    10261 tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa ccgtggggca accggcgttt
    10321 tctttatgac actggtgacc atcgcgacta tcgtttactg gatgaacccg gcaggtaacc
    10381 caaccgtcga tatgatttgt atgattgtta tcggcttcct gatctacggt cctgtgatgc
    10441 tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc ggcaggtacg gcagcgggct
    10501 ttaccgggct gtttggttac ctgggcggtt cggtggcggc gagcgcgatt gttggctaca
    10561 ccgtggactt cttcggctgg gatggcggct ttatggtaat gattggcggc agcattctgg
    10621 cggttatctt gttgattgtt gtgatgattg gcgaaaaacg tcgccatgaa caattactgc
    10681 aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctga aaaaccttag catggcgatc
    10741 atgatgagca ctatagtcat gggaagcagt gcaatggcgg cggacagcaa cgaaaaaata
    10801 gtcatcgccc atcgcggtgc cagtggatat ttgccggagc atacgctgcc agcaaaagcg
    10861 atggcgtatg cgcagggagc ggattatctg gaacaggatt tggtgatgac caaagacgac
    10921 catctggttg ttctgcatga ccattatctc gatcgtgtta ctgatgttgc cgatcgtttc
    10981 ccggaccggg cgcgcaaaga cggtcgttac tacgcgatag atttcacgct ggatgaaatt
    11041 aagtcgctga aatttaccga aggtttcgat attgaaaacg gtaaaaaagt gcagacttat
    11101 ccggggcgtt tcccaatggg taagtccgac ttccgggtgc acacctttga agaagagatt
    11161 gaatttgttc aggggttaaa tcactctacc gggaaaaata tcggtattta tcctgaaatc
    11221 aaagcgccgt ggttccatca tcaggaaggg aaggatattg cggcaaaaac gctggaagtg
    11281 ctgaagaaat atggttacac cggtaaggac gacaaagttt atttgcaatg ttttgatgct
    11341 gatgagctga agcgtattaa gaatgagctg gaacctaaaa tgggcatgga tctcaatctg
    11401 gtacagttga ttgcctatac cgactggaat gaaacgcagc agaaacagcc ggatggaagc
    11461 tgggttaatt acaactacga ctggatgttt aagccgggtg ctatgaaaca ggtggcggaa
    11521 tatgccgacg gaatcgggcc ggattaccat atgttgattg aggaaacatc gcagccgggt
    11581 aatatcaaac tcactggcat ggtgcaagat gctcagcaga ataaactggt agtgcatcct
    11641 tataccgtgc ggtcagataa actgcctgaa tacactactg atgtgaatca gttatatgat
    11701 gctctgtata acaaagcggg tgtaaatggg ctgtttactg atttccctga taaggcagta
    11761 aaattcctta ataaagagta atgctactgc aggatggcgt tcatgcgcca tcctgtctca
    11821 catcaaattt ttccattcac cacgagttgt tccgtacacg tttgtagtaa aaacagtgga
    11881 ctgccttcta ccggcttttc tttagccaac aacgaaatct gtcgatagaa ggttggttcg
    11941 tgaggaacgg aacacaattc acctgcaatg cttttcagcg ttaactctgg ttgtaaggcg
    12001 atgcctaatc cctgtcgaat aaacttcatc gctgtatccg actgattaaa ttcacatttt
    12061 atctactggg cgcactatta gtggtagtgg ctggtggaat ctatgcgctg caaatgaagg
    12121 gtaaaagcgg tgtcacgcta gtagtggcaa aagaaattca ttaatacgac acgtttcatt
    12181 aagattttcc tcaggtaacc acctatagtc attgtcgtta ataaaaatga caggcgatag
    12241 ggtatggcag tttcagcaaa gtacgacgaa tttaatcact ggtgggcaac agagggcgac
    12301 tgggttgaag agcctaacta tcgccgtaat ggtatgagcg gggtgcaatg cgtcgagcgc
    12361 aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc tgtttcattc cgtacgttat
    12421 ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa ttaaagaact ggaacgggcc
    12481 ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga aaattgaggg tgaatggcga
    12541 gcgttgctgg tgactgaaga tatggcgggg ttcatcagca ttgctgactg gtatgcccag
    12601 catgcagtat cgccttattc tgacgaagta cggcaagcca tgttgaaagc agtggcgctg
    12661 gcgtttaaga aaatgcatag cattaatcgt cagcatggct gttgttatgt tcgccatatc
    12721 tatgtgaaaa cagaaggtaa tgcagaagct ggttttcttg acctggagaa aagtcgtcgc
    12781 cgcttgcgtc gcgataaagc gattaatcat gacttccgcc agctggaaaa atatctggag
    12841 ccaatcccca aagcggactg ggagcaggtg aaagcgtatt actacgccat gtaatcgttg
    12901 taggccagat aagacgcata agcgttgtat ctggcactac aaccaattca catctcgatt
    12961 tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt cccccggctg aataaaggct
    13021 aacggttcgg caatccagtc aacctgacct gcaaccagac gggtgcgaca ggagccgcag
    13081 taaccttcgc gacactgata ctcaaccgcc acattgtggg attccagcgc cgccagaagg
    13141 gaagggtgtt catcctggca cagcagttgt gtgccagtga tgcgcagggt aacgcgggcc
    13201 atcagagttg gaagttactc aaatcgtcgg tgtccacttc cgcgtcaatc tgcccgacca
    13261 gataagaact gacttccact tcctgcggag caacctgcac gttatcagac accagccagg
    13321 tgttgatcca tgggatcggg ttggagcgcg tctggaacgg cagatccaaa ccgactgcct
    13381 gcatacggat attggtgatg tattcaacgt actggcagag aatgtcttta ttcagaccaa
    13441 tcatcgaacc gtcgcggaac agataatccg cccagtcttt ctcctgttga gctgcctgaa
    13501 caaacaggtc atagcactcc tgcttacact cttcggcaat ttccgccatc tcaggatcgt
    13561 ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc ggtcaggtgc agggcttcgt
    13621 cgcgggcaat caggcgaata attttggcgt tgccttccat caattcgcgt tctgcaaatg
    13681 cgaaggaaca agcaaagctg acgtagaaac gaatcgcttc cagcgcgtta acgctcatca
    13741 ggcagagata cagttttttc ttcagctcgc gcaggctaac ggtcacagtt ttaccgttaa
    13801 cggtgtgggt accttcgccc agcagatgcc agtagctggt catttcgatc agctcatcgt
    13861 aatagctgga gatcccttcc gcacgtttct ggatctgctc gttggtgacg atatcgtcaa
    13921 acacaacaga cggatcgtta acgatattac gaatgatatg agtataggaa cgggaatgaa
    13981 tcgtttctga gaacgcccag gtttcgaccc aggtctccag ttccgggatc gagatcagcg
    14041 gcagcagtgc cacgttcggg ctacggccct gaatggaatc cagcagcgtc tgatatttca
    14101 ggttgctgat aaagatgtgt ttttcgtgtt caggcagtgc ctggtaatcg atacggtcgc
    14161 gagaaacgtc aacttcttcc ggacgccaga agaaagagag ctgcttttcg atcagctttt
    14221 cgaagatgtc atatttttgc tgatcgtagc gagccacgtt gaccggctga ccaaagaaca
    14281 tcggttcttt gagctgatca tttttcgtct gtgaaaaggt ggtatatgcc atgagtgtgt
    14341 cctgtggttt tattacccct caccctaacc ctctcccgca aggggagagg ggacggtttg
    14401 gtgcaaaggc tgggggatta tcagatctta catgcgccgc tttcgcagcc atcgtcctgg
    14461 attgacggta ccagatcgtc ctgcgcgtct tcagcgccgt cacgggtgtt ctgataatac
    14521 agcgttttga ccccgaattt gtaggcggtg agcaggtctt tcagcaactg ctgcatcggc
    14581 acttttcctg acgggaaacg tgacggatcg tagttggtgt tagcagagat cgactgatcg
    14641 ataaatttct gcatgatacc taccagttgc aggtagccat cgttacccgg catttcccac
    14701 agcagctcat aggcgtcgtg caggtgctcg tagtccggca ccacctggcg caaaataccg
    14761 tctttcgacg ctttgatgct gacgtaaccg cgcggcggtt caataccgtt agtggcgtta
    14821 gagatctgtg aagaagtctc tgacggcatc agggcagaaa gcgtggagtt acgcaggccg
    14881 tgcgttttga ttgattcacg caatgcttcc cagtcgtaat gcagcggctc attagcgatg
    14941 gtatccagat ctttcttata ggtatcgatc ggcaggatcc ctttcgcgta agtggtttcg
    15001 ttaaaccacg ggcacgcgcc ttgctctttt gccagctcgt tagacgcttt cagcaggtaa
    15061 tactgaatcg cttcgaaggt tttatgcgtc aggttgttgg cgctgccgtc ggagtaacgc
    15121 acaccgtgct tcgccaggta gtaagcgaag ttgatcacac caatacccag cgcacgacga
    15181 cccatcgctc cacgtttggc ggccgggatc gggtaatcct gataatccag cagcgcgtca
    15241 agtgcacgaa ccgccagaat tgccagctct tccagttcat ccaggctatt aattgcgccc
    15301 aggttgaaag cagacagcgt acacagcgcg atttcaccgt tctcgtcgtt gacgtcgttc
    15361 agcggtttgg tcggcagggc tatctccagg cacaggttag actgacgcac tggcgcgatg
    15421 gccggatcaa acgggctatg ggtattgcag tggtcaacgt tctgaatata gatacgaccg
    15481 gtagacgcac gttcctgcat catcagcgag aacagctcaa cggctttcac acgctgcttg
    15541 cggatgctgt cgtctttctc atatttggta tacaggcgtt caaactcttc ctgatcggcg
    15601 aagaatgcgt cgtacagccc cggtacgtcg gacgggctga acagggtgat atcttcacct
    15661 ttcagcaggc gggtatacat cagtttgttg atctgtaccc cgtagtccat atggcgcacg
    15721 cggttgcctt ccacaccacg gttgtttttc aacaccagca ggctttccac ttccagatgc
    15781 cacatcgggt agaacagcgt tgccgcaccg ccgcgcacgc cgccctgaga gcaggatttc
    15841 accgctgtct ggaaatgttt gtagaacgga atacagccgg tatggaacgc ttcaccaccg
    15901 cgaatcgggc tacccagcgc acgaatacgc ccggcgttga tgccgatccc ggcacgctgg
    15961 gaaacgtatt tcacaatcgc gctggaggta gcgttgatgg aatccagact gtcaccgcac
    16021 tcgatcagta cgcaggagct gaactgacga gtcggggtac gcacgccgga catgattggc
    16081 gtcggcagcg aaattttaaa tgtggaaacc gcgtcgtaaa aacgcttcac atattgcagg
    16141 cgcgtttcac gcgggtagtt cgagaacagg cacgcggcaa ccagaatata aaggaactgg
    16201 gcgctttcgt agatttcgcc ggtcacgcgg ttctgtacca gatatttgcc ttccagctgc
    16261 ttaacggcag cataagagaa ggtcatatca cggtcgtgat cgataaacgt gtccatctgc
    16321 ttgaactctt cttccgtgta gtcttccagc agatgattat cgtatttgcc catctcgacc
    16381 attttcacca cgtggtcgta cagcgcaggc ggctcaaact ggccgtaggc ttttttacgc
    16441 aggtggaaga tcgccaggcg cgcggcgaga tactgataat ccggcgcatc acgggagatc
    16501 aggtctgcgg ccgccttgat aatggtttcg tggatgtcag aggtcttgat accgtcataa
    16561 aactgaatgt gggagcgcag ctcgacctgg gaaatcgaaa cgttatgcag tccttctgcc
    16621 gcccaatcca gaacgcgatg gattttgtcg agattgatgc gctctgtgct accgtcgcgc
    16681 tttgtcacca gcagattctg attcatgtat gtcgtacctg tttttggaat tctttccgca
    16741 atagtttcat gcagactacc tgtagtacgg gaagctgctt agaacactat atataggggg
    16801 tatgtttggg attcactgca agatagtgtg aaaatggccc tcttgcaagt gcataacttt
    16861 gtggataact caggaaggaa aaagtggctt tcgcgcacct taggtcagac aaggtgtccg
    16921 ggaaagtcaa tgggaagaga aaaatttgtt aaaaataact gttcggcgat ttcttgaacg
    16981 gcagaatagg ttggataaat tgatatagat ggtttattaa tctttttgct aatgattgca
    17041 aaaattgatc taagcacgag tcatcacata atctgatgta tcagttgtgg tcaatatata
    17101 agcgaatgta agcgattgtt gatgtcgaaa tggttgtttt ctattcggtt attgttaaa
//
LOCUS       DAZFDP010000106        16903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFDP010000106 DAZFDP010000000
VERSION     DAZFDP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(160..2151)
                     /gene="tkt"
                     /locus_tag="ACHJ1G_003476"
     CDS             complement(160..2151)
                     /gene="tkt"
                     /locus_tag="ACHJ1G_003476"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4765270.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2429..3187
                     /gene="loiP"
                     /locus_tag="ACHJ1G_003477"
                     /gene_synonym="yggG"
     CDS             2429..3187
                     /gene="loiP"
                     /locus_tag="ACHJ1G_003477"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4765271.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3243..3986)
                     /locus_tag="ACHJ1G_003478"
     CDS             complement(3243..3986)
                     /locus_tag="ACHJ1G_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4765272.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3973..5082)
                     /gene="dgaE"
                     /locus_tag="ACHJ1G_003479"
     CDS             complement(3973..5082)
                     /gene="dgaE"
                     /locus_tag="ACHJ1G_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO4765273.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5086..5946)
                     /locus_tag="ACHJ1G_003480"
     CDS             complement(5086..5946)
                     /locus_tag="ACHJ1G_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4765274.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5943..6692)
                     /locus_tag="ACHJ1G_003481"
     CDS             complement(5943..6692)
                     /locus_tag="ACHJ1G_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO4765275.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6718..7203)
                     /locus_tag="ACHJ1G_003482"
     CDS             complement(6718..7203)
                     /locus_tag="ACHJ1G_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4765276.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7214..7642)
                     /locus_tag="ACHJ1G_003483"
     CDS             complement(7214..7642)
                     /locus_tag="ACHJ1G_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4765277.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7761..10559)
                     /gene="dagR"
                     /locus_tag="ACHJ1G_003484"
     CDS             complement(7761..10559)
                     /gene="dagR"
                     /locus_tag="ACHJ1G_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO4765278.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10818..11738)
                     /gene="speB"
                     /locus_tag="ACHJ1G_003485"
     CDS             complement(10818..11738)
                     /gene="speB"
                     /locus_tag="ACHJ1G_003485"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4765279.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11874..12605)
                     /locus_tag="ACHJ1G_003486"
     CDS             complement(11874..12605)
                     /locus_tag="ACHJ1G_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765280.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12751..14727)
                     /gene="speA"
                     /locus_tag="ACHJ1G_003487"
     CDS             complement(12751..14727)
                     /gene="speA"
                     /locus_tag="ACHJ1G_003487"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4765281.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14736..14867)
                     /gene="yqgB"
                     /locus_tag="ACHJ1G_003488"
     CDS             complement(14736..14867)
                     /gene="yqgB"
                     /locus_tag="ACHJ1G_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4765282.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14961..15104)
                     /gene="yqgG"
                     /locus_tag="ACHJ1G_003489"
     CDS             complement(14961..15104)
                     /gene="yqgG"
                     /locus_tag="ACHJ1G_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4765283.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15215..15466)
                     /gene="yqgD"
                     /locus_tag="ACHJ1G_003490"
     CDS             complement(15215..15466)
                     /gene="yqgD"
                     /locus_tag="ACHJ1G_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4765284.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15522..16676
                     /gene="metK"
                     /locus_tag="ACHJ1G_003491"
     CDS             15522..16676
                     /gene="metK"
                     /locus_tag="ACHJ1G_003491"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4765285.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         4139 a         4669 c         4220 g         3875 t
ORIGIN      
        1 atgtcaatac gcatatcgtg atgcatatta cataattgat atttattgca tataaatatt
       61 tgtgatctac aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa
      121 aaaaggtcgc cgaagcgacc tttttacccg aaatgctaat tacagcagtt cttttgcttt
      181 cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga
      241 ttcaccgaag gtggtcatac cgacgatagc accgttcagg ccaacatact tgtaccagta
      301 gtcagcaata cccgcttcta cagcaacgcg tgcagtaacc gctttcggca gtacggattc
      361 acggtaagca gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc
      421 tttcacgcct tcggcagtca gtttttcgta ggcagcaaca gccagttcaa cttctgaacc
      481 ggtagcgatg aaaatcagtt ccggctgacc ggcgcagtct ttcagcacat aaccaccgcg
      541 cgcgatgttt gccagttgct cttcagttcg ttcctgctgc gccaggttct gacgggagag
      601 gatcagtgcg gtcgggccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc
      661 aacctggtca cacggacgcc atgtagacat gttcggggtt acgcgcagag aagcgacctg
      721 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
      781 catcacctga cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
      841 gaacatcagg aaggtggagg tgtacggcag gaaaccaccg tgcagggaga taccgttagc
      901 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc
      961 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagaacc
     1021 gccgaggaat tccggcaaca gcggaccgaa agcttcgata gcattctgag acgctttacg
     1081 gctggcgatt ttcgccgggt tagcctgcag tttagcaatg aactcttttg ctttcgcgtc
     1141 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagcggctt cctgcggata
     1201 agctttcgcg taagcagcga atttctcgtt ccatgcggat tctttcgcct ggcctgcttc
     1261 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     1321 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     1381 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     1441 tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atggatgccg cgtcatgacc
     1501 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt
     1561 gaaccagcct tcaacgtgac catcaataga gataccgttg tcatcgtaga acgcaatcag
     1621 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     1681 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     1741 aaactgcgcc gccagcgttt tttctgcaat cgccatacct actgcgttgg caataccctg
     1801 ccccagcgga ccggtggtgg tttccacacc agcggtgtaa cccacttccg ggtggcccgg
     1861 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     1921 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     1981 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     2041 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     2101 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     2161 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     2221 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     2281 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg
     2341 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     2401 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     2461 cgtggcaacg gtactgactg gttgccagaa tatggactcc aacggactgc tctcatcagg
     2521 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     2581 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     2641 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     2701 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     2761 ctatagcggg ctgatggata tgatgacgga taacgaagtc gaagcggtga tcggtcacga
     2821 aatggggcac gtggcgttag gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa
     2881 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggaagtttat ctcaatcaca
     2941 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga
     3001 agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc
     3061 caccagcttt gaaaaactgg caaaactgga agaaggtcgc caaagctcaa tgtttgacga
     3121 ccatcctgca tccgccgaac gcgcccagca tattcgcgat cgtatgagcg cggatggggt
     3181 taagtaagtc agacataccg atctacaaca taattgtagg tcggtaacgc ctgaatcccc
     3241 gatcagagta gtgatttcac tttatttagc aaaacggcta catcatccgg tcgggtatat
     3301 cccgtatcag ggtctataat cgagctgtaa acatgtggca taatgcgcgg cacccccgcc
     3361 tccagacaag ttttcagaat cacactgaag ttatccaaat caatcccgcc agtcggctca
     3421 atcagcgtca taccattgcg cgccgccgtg gtcgccagcg cgtatagctc cggaagcgac
     3481 ctctcgccgc ccatcggaaa gaacttcgcc gcgtgtgcgc ccatatctag catcattcgc
     3541 accgccgtat cgcaggagac gcgggcaggc gtaccctggc tgctgctaac gccggtggaa
     3601 atcagcactt cgcccggcgt ccccgtcggg ctaaccagcg cgttgacatg ggtctgctca
     3661 ccgcccgtag ccgcaagcgc gcctgccgcg aaaccacaac cggtaaaggt ctggttaacg
     3721 tgtgctggat gaatctgcgc cgcaatcatc gcagctttgt aatactgcgc cgggtcaccc
     3781 gcccccagac ctactgaaat ggacggtatt tccgccatcc aacgtttaac ttcctggatc
     3841 ccctcatcca ccgaggagaa ctgcgcggaa agcacgccaa tcactgcgtg gccttcggcg
     3901 gcgtcaaaga tttcacgggc attggcgata tctttcgcca gcacattaat tgccacgcga
     3961 ttacgataga aattaatctt ttgcatgatc tgcaatctcc ttcagtcgcg ccacgataag
     4021 tgccatttcc ccttccgcca ccgtacgcgg atcaaggcta aaaacgccct ggtggaggtt
     4081 ataccgccgt gcataaatcg cgatatcgcc accacgcagt tgcgcttcga cgacgcgagc
     4141 gtcaactccc aattcctgcg catttactcg aatacgaata cgccagattg cacgcccggc
     4201 ttcatcctgc tcaatatcag ccgttaaccc gtgaatcgca gaaatcgctt caaccactgg
     4261 ctggagctgt tccgcggtga tgactgcctg cccctggtga taattctcca gcgcgtaaac
     4321 cagcccaacc atgttctcct tgccgatttt catcgccctg gcaatgccgt gatgctgcgc
     4381 tttgcaggct gcaatccaga tttttttacc agtgataaac ccggaggtcg gcgcgttaaa
     4441 ggcttttgcg ccgctgtaga ccaccatatc cgctccgctc accacccagg cacgtaaatc
     4501 ctcttcggcg gcggcatcga caatcagcgg caggttgtac atctgcgcga catgtgcgaa
     4561 gtcctcgatg ttcaacattc ccttctgcac gcagtgatgc gacttcacat acagcagcgc
     4621 ggcggtcgcg tcggtaatgg cactctccag ctgccagaga gccgccaggt tacttgaacc
     4681 cacctccaca acgcgcccgc cacctaagcg gatcgcgctg aggatcgatg caccgtaatc
     4741 gacgttatgg ccgcgcagca tcaccacttc attggccatg ccggagctat ccggcatgag
     4801 cgccacccgg gccttgtcgc cgcgtgtaat ggcggcggcg acagcgatgg cgatccctgc
     4861 tgaggcacag gaggtaacgt agctatcctc cgccccggta aagcgagaga ccagctcgcc
     4921 ggttttgtcc accagctggt caatctccac gaaagaggat gccgcgcgcg cggtggcctg
     4981 catcacctcc ggcgcgacgc tggagacgcc gagaatcgtc atcttgccgc aggcgttaat
     5041 cacctgtttc agtcccagtt gttgatagat attctgcgtc atggcttaca gcactcccag
     5101 cagcgagcag accacgctca acgccacaat cgacagcaga atcgtggtgt aacgcggacc
     5161 ctttttcacc aaatagaagt agatggcgaa cactgccgcc agcggcagca ggccgggcgc
     5221 gatggagtcc agaatctgct gtaccaccac ttcagagcct ttcagtgcgc taatttttaa
     5281 aggtgaggtt atcttgacaa agctcgcgga tagcgcgccc atcatgataa gccccagtac
     5341 gttagcgccg tagataagct ccttgatgcg ccctccctgc agcagactga taatcgaatc
     5401 gcgccccagc gtatagccct tgtgcaccat gccgtagctg attgccagcg taatcgccgg
     5461 gtagagaatc agcggcaaaa taccgcccat cgcgctgcca ttggcggcga acgggataaa
     5521 aatagcaatc aacagcggca tcaccgccgc ccagatgatg gagtcgccca tcccggccag
     5581 cggccccatc aggccggttt taatcccggt gatagaggca tcgttaatcg cctcgccgcg
     5641 ggtcttttgc tcttccatgg cgatggagat cccctggatc accgcgccaa acgtctgctc
     5701 tgagttgaag aagttcagat ggcgttttaa cgcctccacc tgctcctcct tttgcggata
     5761 gagctttttg ataatcggcg tcatcgaggc gcagaaaatc aggctctgta aacgctcata
     5821 ggagctggaa acttccgcgc ccagccagta gataaaccac gctttggtga tatccgcttt
     5881 ggtcagcgcc ccggactccc gcgcgcgctc aaccagttca tgctgcatta cgtcgtcgct
     5941 cattatgccg ctccttcatt tttcgccagg cctttaatca ggaaggccac gcaggtgccg
     6001 aaaatcgcca tcgccatgat gtccaccttg aggtacagca ccgcgaagaa tccgccgata
     6061 aaccacggca gcaggctctt tttaccaatc accataatgg tgatggcgaa gcccagcgcc
     6121 gggagaatcc cgcccatgat ttcaaacgag tgggtcagcc agtgcggcat cagcttgagg
     6181 aaactttcca ctacgttctg gccgaagtag ttggcggcga acaccactgg gaaacgtagt
     6241 accagcccca gcagcgccgg gtagagaaag gcgcagcgca taatggctga catattggcc
     6301 gtttccgcgt gtttatcggc catatgcacc caggccgcat ttagcgtgcg acgcaactgg
     6361 tcgaggaata cgccgataac gccaaatgga atcgccagtg caatcgccag gttcgggtcc
     6421 atattggcct taacggcgat aggaatggcg atacaggccg ccagcgccgg gtctgacggc
     6481 acattgccgc ctggcgttga ggtcacaccc agatagacca actgcatccc cgcgccgata
     6541 atcatcgccg caggcatatt gccgagcagc aggccgacaa agaccgccac caccactggc
     6601 tgtaacagca tggcggagaa ggtatagcca aggcgcaacc gggcaaacca gtaatacagc
     6661 cccatcaggc ttgcaaaaag taaggtatcc ataatttgtt atcccatgtt cagagggtca
     6721 gaattttttc aggatatcgt ccagcgactg cggtttatct tccgggatgg tctggaaaac
     6781 agccttaatg ccgcgacttt tcaaatcgtt aagaatgcca acgtcttttt catccagcgt
     6841 tatgttctgg aacaccgctt tacggtttgg cccgccgcct aaaccgccaa cctgaatatc
     6901 agccacgtca aagccctgct gcaccgcttc ctgaatcgcc gccagcgacg ggaacagcac
     6961 cagcacgttg ccgtcgccca gctggttctc tttccacgcg gccgcgaagc tctggttgct
     7021 aaagcagtcc actttgatat tcggcggcgc ggccatcagg tagatatttt tcataaacgg
     7081 atcggcgtcc agctcgtcgc tgaccaccgc aatacggttg gcctgagact gccctaccca
     7141 cttcgtcacc acctgtccgt ggataagacg gctgtcaatg cgacataaca cgatgtttgc
     7201 catacgattt tccttactgt tgtaattgac gaatatggga gacgacgtcc aggcaactgt
     7261 tgcgcccagc ttccaccaga tcggccacgc aggcggtcag tgggccatgt tcgcgcttgt
     7321 ccagcgcctc cagcagcagc gaggcattaa gcccggagat caccgccacc ggataatcgg
     7381 cgctcagccg cgccgccacg ttagaggtgg tgccgccgat aaaatcggtc acgataagcg
     7441 aaccttccgg catggttttc accacggctt cgacgcgctg gtaaaactcg cccagcgtat
     7501 cgacgggcat cagcgcgatt tccgtcacgc cttcgatttc gcccatcacc atgcgcagac
     7561 tgttgcagag ctgcagcccc cagccgccgt gagttagcag cagaatttca ggtaacggat
     7621 gaataggtac agttgtggtc aaaatagcct cctttgctcc agtgtttaca tttcatcagg
     7681 agcaaaggac gtgccagaat ttgtgtcacg cacagaggaa atgagattgg atggggcgtt
     7741 aagccccgta aaccagggga tcagctgtag agcagttcgt agatataaaa atattctgcg
     7801 tccgataaac ggatagcgta agcctcttca atcggcaaaa aggaggattt gatgacgcta
     7861 aaggcacgag cgtccagatc aagccggttt tctagcgcca tctgtaacgg tttgcggtta
     7921 atcacgatac gctcgaccat gcagcagcag tggatcagaa aacgtagtgt cacctggcgg
     7981 ctcggtttca gggagagcgt gttggttaag tagttcagaa catcctcgat ctctttcaga
     8041 attcgctgcg ggttgagcac cgaaatatgg ttgatgatgc tctccatggt cagcgcgctg
     8101 ataaagcgca tggcgctgcg ctccatctcc agtcggcgct cgctgcttga gagatccggc
     8161 gtcagcaggc tgagcaccag ctccggccct tgctcggaaa acagttcctc cagcgagata
     8221 aacggtatat ccggcagtcc cggctggaag gtgccgacga tccctgccag ccgttcactg
     8281 gcattaagcg cctgctgaat acgctccagg ctgcgcacct cgttatagtc gagaatcacc
     8341 atacgcgtgt cctgcgacat cagctcgcca aagctctcct ccagcacctt tttgatcttt
     8401 tccgccgtcc ccattccggt tatacatgag ataaccagca ctttgccacc gctctcttgc
     8461 tgcggcgtgc agagccgaca agggatattt ttactctgca taagcacagc caattgcgat
     8521 agatcgccag tttcgtagct taaatccagc ccaacctcca gcaggctggt cagagtgata
     8581 tttggcaaca acagaacgtc aatctggaac agcttgctga tggtgctgcc aaaatggacc
     8641 agcgatccga tatccaccat cagtatcagc cgctgccagc accggtcctg aatcatccgg
     8701 gtcagggttt ccagggtgtc atgcaccgac tgctcaaatg gcatatcaat tgcgtagaac
     8761 agatcgcgct ccagcacccg gttcacatat tgcgcctggc tcgttgcggt ggtcgcaccg
     8821 tgagcgatta gaatcacgcc gcaatccggg ctggtgtcga tgcgctggcg gtagtggcga
     8881 cactctttca ggaacaggca cagccagacc acctccgtcg ccgggcactg aatatgcaac
     8941 aactcattga ttttgcggca cagaagcgtg gcattgtcat actcatcttt acagcgatcc
     9001 agaatcaggc tggatgaata gagttgaggt ataagccctc gccgcacata gccaatcagc
     9061 gcaagaaagt gtttacgtaa cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc
     9121 tgctccacgt aaccgatcag cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt
     9181 ggatgcgccg cactatggcg gctataaaga ccatattcaa aaatcgaact gagtttgttt
     9241 ttcaggatag ccagcgtttc tgcgggcggc acgttactgt tgcgcagatt gacatattca
     9301 cgagtcagga aactgtaaaa caggtcgctc tcttctattt ccgcgctggt cgccaacgaa
     9361 ttttttaacg cgggcaacgt acgcgcatcc acgttcagac gatctttacc gccaaacaac
     9421 gcatcgacca gcagacgctg ctctggcgtg gtattaaacg acatctccgc cagtcgttta
     9481 tctaactcta gcgtatcatt acgctcggtc attcccgacg cccacgcctg cgcgcacaag
     9541 aactgaatat cacttttaag ctggccgata ttgccttcca ggggtttact taacaaccaa
     9601 tgcaagagcg ttttatcaag gcttatggta cgttcaattt tacgactttc acgctgaaag
     9661 aagcttacaa tcaattcgag ctgctcctcg accgaacgct ggcgaatacc cggtaagtca
     9721 atacatacct ggatacgccg ctggaaggta cgcaatagtg ccgagttgac cgactctgta
     9781 gtggcgcaaa taaggcgcac cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg
     9841 tattcacctt tatcgagaat agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc
     9901 acttcatcca gaagtaaata acctccgtca gcttgctcca ccaatcccgt tttattctca
     9961 cttgccccgg taaatgcccc cttatggtga ccaaacagat gtgaggaaag caactccgga
    10021 ttatgagcat attcggcaca gttaaagtaa accaacggag gtggcgaacc tatggtgtgc
    10081 ttgcaagcga agcaatgcat taactcagca aagaatgttt tgccaacacc agatgcaccg
    10141 gttaacagca catgtagtcc gtggggataa agcaccgctg cgcggccttt ttctaccgca
    10201 tcacggagac tacggtcgta tccgataagt ccggaaaacg gatcatcatc tgtatatttt
    10261 tctttctttg gcaataaact agccactgaa tgaactatgc attcgcaatc atccagtttt
    10321 cgattaatga gcaattctag tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt
    10381 tttaccacca ggccttcatt ccataattga ttaagatctt tactaaccga gttgcgtgcc
    10441 agcccaaggt taaaaccaat cgcctcagca gtaaatgctt tctccttcgc taaatcagac
    10501 agttccagcc catacgtcag ccgctccagc tccttgtgaa taacatcaat ccgtctcatt
    10561 tctttaccgc ttaaaaaaca atagttgttc agaacataca ctatttgtaa acgtaagaat
    10621 gagaactatc gggaagaatt aatgttcctt gtggcaatct ggtttctgaa tattcgtgtt
    10681 ttaaatatta cttatggtct gaaacaacca ggcaggtgaa cagagctaag aaaaagtagc
    10741 gtagaaatat aaaaacatat atcgcactga tgaatgtttc ccggcatgac tatgcacacc
    10801 gggaaatatt tagtaaatta ctcgcccttt ttcgccgcct gaatatacag catctccagc
    10861 gccagcgtcg ccgcagccag agcagtgatt tccgactgat cgtatgccgg agccacttcc
    10921 actacgtcca tcccaacgat gttgagatct ttcaggccgc gtaccagttt aatggcgcga
    10981 tcggaggtca gaccgccaat taccggcgta ccggttcccg gcgcaaatgc aggatccagg
    11041 cagtcgatat caaaggtcag gtaaaccggc atatcaccca caatttgttt cacctgggcg
    11101 ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt aaagccgttg
    11161 tctttatcaa attcggtacg aataccaatc tgcacggaat gattcggatc gatcagacct
    11221 tctttcggcg cggtatagaa catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta
    11281 tcggtgtggg cgtcaaagtg taccagcgcc attttgccga aatgcttcgc atgagcacgc
    11341 agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga gcatacgctt accggcagcc
    11401 agcagcttct cggcgtgcgc ctgcagcttc tcgctcatct cacgggcatc gccgaaagca
    11461 tataccagat caccgcagtc cacgacgttc agacgctcac gcatgtcgaa attccacggg
    11521 aagcggttgt gttcccaggc cagattcgtc gaaacctgac ggatagctgc cggaccgtgg
    11581 cgtccccccg cacgaccaga agtggccata tcgaacggca caccagtaat cacccagtca
    11641 gcatcgctgt catacggctg gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa
    11701 accagtgagt tatcgtattg atgacctaag gtgctcatgg atggactcct attacaaagt
    11761 cgatataaaa aaacccttcc gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa
    11821 cacaagtttg tgctaattat cgccttaaat gggcgaggca tcaagcgagg ttgctatttc
    11881 cgacacggtg gcggcgcata gccattgtta tctgccggaa aggcttgtcc agagcacggc
    11941 ggcagcgcgt tttgcgcctg gccttgtaat tcaaaggcgg caaagcctat tacaccggta
    12001 ttacgcacat cgccttgcgc cgaattggct gcgtaggaat cagcaacgtt ggcgaactga
    12061 aaagccgctt cggtatgttt atctttgcgg aatcctttaa tcgctaaaga atctccggca
    12121 ttcacaatat agccgttgtt attcagcgaa ccttgcttgc cgttcagcac atccaggcca
    12181 tcaacagtag caacgatctc gtagttggtg ttacgactgt aattacggac atataattga
    12241 taattcattc cgactctggc atggacaata tagcgtggtt cattattgga tgaatcataa
    12301 acacgcgtaa taggtagaga attaaaatta ccatcgcgaa tagcatattc cagttcgtta
    12361 atacgaatgc tataaacgcg gtcatagccg gaaggaacat tagtcgaata actaataacg
    12421 atcatctctg ccggatcgcg ccaggctcgc tcagcagata cgccctgaac cgatgagtgc
    12481 acatccccac cccaggcagt gccgacattg cccgatgcac tgctattata ctgcgcatta
    12541 ctggaacaac cggccagcag cacgattaat gccaccaacg cgctacgtac tttccatttt
    12601 ttcataaccg ttccctctgc atttaattaa tgcagcgatt gtagggagat tatttaataa
    12661 gaaaagcagc gagattattt ttaagaatat ataaaaagaa agcgggccga tttacgtaaa
    12721 taaatcgacc cgccagcgga ataatcttaa ttattcatct tcaagatagg tataaccgta
    12781 caaacctgcc tcgaactctt caaggaactg ttgttgcagt tcagcatcaa gatcggtttt
    12841 cttcacttga tcgcggaact gggttaacag cgttttcgga tcgagctgta catattgcag
    12901 catgtccgcc acggtatcgc cttcgtcaga cagttctact tctacgctac cgtcagggaa
    12961 cacgaacacg tcaaccgctt cggtatcacc gaacaggttg tgcatattgc caaggatctc
    13021 ctgatatgcg ccgaccataa agaaaccgag catcggcgga ttctctggat cgtactccgg
    13081 cattggcatt gtcgtggcaa taccatcacc atcaatatag tggtcgatag caccgtcaga
    13141 gtcacaggta atatccagca gcactgcgcg acgttccggc acttgatcca gcccttccag
    13201 cggcagaacc gggaacaact ggtcgatccc ccatgcatcc ggcattgact ggaacagcga
    13261 gaaattgacg tacattttgt ccgccatacg ttcctgcagt tcgtcgatta tcggacgatg
    13321 cgcacggttt tgcggatcca gctgcttttg cacttcatgg cacatgctca gatagagttg
    13381 ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc
    13441 gtgcagatcc atctggctgt cgtgtaacca ttcacgcaga gaacggcgag ttcccggttc
    13501 gtgcatttcc tgccaggttt cccacatgct ttgcagcgcg cgcggcgcat cttctgcagg
    13561 cgcggtcggc accgtgtatt cgttacgttc cacgccgatg atattagata ccagcacggt
    13621 gtgatgcgca gtcaccgcac gacccgattc ggtgattacc gtcggatgcg gcagaccgtt
    13681 ttcttcacac gcatcaccaa tcgcccagat gatgttgttg gcatattcat tgaggccgta
    13741 gttcaccgaa cagtcggact gcgaacgggt gccttcatag tccacgccca gaccgccgcc
    13801 gacgtcgaag cactgaatat tgacgcccag cttgtgcagt tccacataga aacgcgcgga
    13861 ttcacgaacg cctgtcgcga tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg
    13921 cagcagttgc aggctgtcga gacgcccggc ttcacgcagg gtttcaacaa gttgcagtac
    13981 ctgagtcgca gccaggccga acttcgattt ttccccgccg gaggactgcc atttacccga
    14041 gccctgcgaa gccagacgtg cacgcacgcc cagacgagga acgacattca gacgttctgc
    14101 ttcatccagc acaatggcga tttctgacat cttctcaatg accaggtaga ccttgtggcc
    14161 catcttctcg ccaattaatg ccaggcggat atattcgcgg tctttataac cgttgcagac
    14221 gatgacgcta cgggtcatgc cagcatgtgc cagcaccgcc atcaactctg ctttcgagcc
    14281 tgcttccagt cccagcggtt cgcccgaatg aatcagggac tcaatcacgc ggcggtgctg
    14341 gttaactttg atcggataaa caaggaagta atcgccgtta tagccgtagg attcccgcgc
    14401 acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa
    14461 cagtgcaggc agacgctgac cctgtgcttc acgagttttc actaactgcg cgagatcgac
    14521 gcgagcttcc gggacgtccg ggtccgggca cacgctgatg tggcccagct cgttaacgtc
    14581 atagtagtta ttgccccacc aggcaatatt gtaagtacgc agcatcttgc tggcttcctg
    14641 ggagctcatt gcaacctcct gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg
    14701 caaacccata gacatgtcgt cagacatagc gaacctcaaa ttattttatt aagtgtaaaa
    14761 cagttaacga ctatcgcagc ctggaactgc gataacaacc cataagcaca tggattttcc
    14821 agcagtgtat gctgacgctc cgactgcgca accggtttct ttttcataac attattaagc
    14881 acataaccga acgtaagtgt gacagttcgg cgaaaccacg agaaaactct tgtttttaca
    14941 agagcgccct tgttcagtcc tcagtaactg taaccagctc ttgaatcctg agaagcgccg
    15001 agatgggtat aacatcggca ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct
    15061 tcaccagaac ggtgagacag gttaagcagc agacaacggt tcattatttc gtatcacctc
    15121 cacggccgcc tgttaagacg aacccacaag ccaaaactct gatttcaacc cggctggaag
    15181 tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc
    15241 ccacaatggc agaaaattgc aaaagataaa tacgcagaat gccggcattg tcaggaaaaa
    15301 tttccagcca cgtttttaac agaatgagac acgattcaaa aaaaagtgga aatcgggtga
    15361 agaattgacc taaaatagcc atccagatgt taatccatcc ataccgatta acactcagac
    15421 tgccagtgtt tttaacttgc agagtcgtgg taggatccgc taccacagaa aatccacaca
    15481 acagtttgag ctaaccaaat tctctttagg tgatattaaa tatggcaaaa caccttttta
    15541 cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc tgaccaaatt tctgatgccg
    15601 ttttagacgc gatcctcgaa caggatccga aagcacgcgt tgcttgcgaa acctacgtaa
    15661 aaaccggcat ggttttagtt ggcggcgaaa tcaccactag cgcctgggta gacatcgaag
    15721 agatcacccg taacaccgtt cgcgaaattg gctatgtaca ttccgacatg ggctttgacg
    15781 ctaactcctg tgcggttctg agcgctatcg gcaaacagtc tcctgacatc aaccagggcg
    15841 ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca gggtctgatg tttggctacg
    15901 caactaatga aaccgacgtg ctgatgccag cacctatcac ctatgcacac cgtctggtac
    15961 agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa
    16021 gccaggtgac tttccagtat gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt
    16081 ccactcagca ctctgaagag atcgaccaga aatcgctgca agaagcggta atggaagaga
    16141 tcatcaagcc gattctgccc gctgaatggc tgacttctgc caccaaattc ttcatcaacc
    16201 cgaccggtcg ttttgttatc ggtggcccga tgggtgactg cggtctgact ggtcgtaaaa
    16261 ttatcgttga tacctacggc ggcatggcac gtcacggtgg cggagcattc tctggtaaag
    16321 atccatcaaa agtggaccgt tccgctgcgt acgcagcacg ttatgttgca aaaaacatcg
    16381 ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg
    16441 aaccgacctc catcatggta gaaactttcg gtaccgagaa agtgccttct gaacaactga
    16501 ctctgctggt acgtgagttc ttcgacctgc gcccatacgg tctgattcag atgctggatc
    16561 tgctgcaccc gatctacaaa gaaaccgcag catacggtca ctttggtcgt gaacatttcc
    16621 cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt
    16681 tcttcacctg ctttcaaagg ccagcctcgc gctggccttt ttcttttacc tcaccgcggt
    16741 ggataggcat tcacgccgca tccggcaaaa agctgcccgc acaataacat cattcttcct
    16801 caacacgttt tatcgcaaat tatcatcact actgaaaccg attacaccaa taacaccagc
    16861 caaatatttg taatattttc atattattat tcagttttca cag
//
LOCUS       DAZFDP010000107        16718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFDP010000107 DAZFDP010000000
VERSION     DAZFDP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..2429
                     /gene="ynfE"
                     /locus_tag="ACHJ1G_003492"
                     /pseudo
     CDS             4..2429
                     /gene="ynfE"
                     /locus_tag="ACHJ1G_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            2490..4913
                     /gene="ynfF"
                     /locus_tag="ACHJ1G_003493"
     CDS             2490..4913
                     /gene="ynfF"
                     /locus_tag="ACHJ1G_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4765286.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4924..5541
                     /gene="ynfG"
                     /locus_tag="ACHJ1G_003494"
     CDS             4924..5541
                     /gene="ynfG"
                     /locus_tag="ACHJ1G_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4765287.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5543..6397
                     /gene="ynfH"
                     /locus_tag="ACHJ1G_003495"
     CDS             5543..6397
                     /gene="ynfH"
                     /locus_tag="ACHJ1G_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4765288.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6440..7054
                     /gene="dmsD"
                     /locus_tag="ACHJ1G_003496"
     CDS             6440..7054
                     /gene="dmsD"
                     /locus_tag="ACHJ1G_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4765289.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7213..8505
                     /gene="clcB"
                     /locus_tag="ACHJ1G_003497"
     CDS             7213..8505
                     /gene="clcB"
                     /locus_tag="ACHJ1G_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4765290.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8458..9153)
                     /gene="bioD"
                     /locus_tag="ACHJ1G_003498"
     CDS             complement(8458..9153)
                     /gene="bioD"
                     /locus_tag="ACHJ1G_003498"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4765291.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9278..10498)
                     /gene="mlc"
                     /locus_tag="ACHJ1G_003499"
                     /gene_synonym="dgsA"
     CDS             complement(9278..10498)
                     /gene="mlc"
                     /locus_tag="ACHJ1G_003499"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4765292.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10633..11526)
                     /gene="ynfL"
                     /locus_tag="ACHJ1G_003500"
     CDS             complement(10633..11526)
                     /gene="ynfL"
                     /locus_tag="ACHJ1G_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4765293.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11633..12886
                     /gene="ynfM"
                     /locus_tag="ACHJ1G_003501"
     CDS             11633..12886
                     /gene="ynfM"
                     /locus_tag="ACHJ1G_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4765294.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(12935..13054)
                     /locus_tag="ACHJ1G_003502"
     CDS             complement(12935..13054)
                     /locus_tag="ACHJ1G_003502"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4765295.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13310..13618
                     /gene="asr"
                     /locus_tag="ACHJ1G_003503"
     CDS             13310..13618
                     /gene="asr"
                     /locus_tag="ACHJ1G_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4765296.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            13711..13794
                     /locus_tag="ACHJ1G_003504"
     CDS             13711..13794
                     /locus_tag="ACHJ1G_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765297.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13894..14715
                     /gene="ydgD"
                     /locus_tag="ACHJ1G_003505"
     CDS             13894..14715
                     /gene="ydgD"
                     /locus_tag="ACHJ1G_003505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4765298.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14754..15083)
                     /gene="mdtI"
                     /locus_tag="ACHJ1G_003506"
     CDS             complement(14754..15083)
                     /gene="mdtI"
                     /locus_tag="ACHJ1G_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4765299.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15070..15435)
                     /gene="mdtJ"
                     /locus_tag="ACHJ1G_003507"
     CDS             complement(15070..15435)
                     /gene="mdtJ"
                     /locus_tag="ACHJ1G_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4765300.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15847..15990
                     /locus_tag="ACHJ1G_003508"
                     /pseudo
     CDS             15847..15990
                     /locus_tag="ACHJ1G_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
BASE COUNT         4141 a         4256 c         4162 g         4159 t
ORIGIN      
        1 gttatgtcca aaaatgaccg aatggtgggc atcagccgca gaacactcgt taaatcaacc
       61 gcgataggtt ctctggcgct ggctgcaggc ggtttttctt taccgttcac cctgcgcagt
      121 gcagcggcaa cggtacaaca ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac
      181 tgtggtagcc gctgtgcact tcgtctacat gttaaagata atgaagtgac ctgggtggaa
      241 actgacaata ccggcagcga tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt
      301 cgctccatcc gccggcgtat taatcatccc gatcgcttga attacccaat gaaacgcgtg
      361 ggtacacgcg gtgaaggcaa attcgaacgg attagctggg atgaagccct ggatactatc
      421 gccagtagcc tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata tattcagtac
      481 tcttcgggga ttgttggcgg caatatgacc cgctcttcgc catcagcctc ggcggtcaaa
      541 cgcctgatga actgctacgg cggttcactc aatcagtatg gctcctacag caccgcgcaa
      601 atttcctgtg ccatgcccta cacctacggc agtaatgatg gcaacagcac cacggatatt
      661 gaaaacagca agctggtcgt gatgtttggt aacaacccgg cagaaacccg catgagcggc
      721 ggtggcatca cttatcttct tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt
      781 atcgatccgc gatataccga tacggctgct ggtcgtgaag atgaatggct ccctattcgc
      841 ccgggcaccg atgccgcgct ggttgcgggt attgcctggg tattgattaa cgaaaatctc
      901 gttgatcaac cttttctcga taaatactgc gtcggctatg acgaaaaaac cttacccgca
      961 gatgcaccca aaaatggtca ctataaagcc tatattcttg gtgaaggtga tgataacaca
     1021 gcgaaaacgc cgcagtgggc ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa
     1081 ctggcgcgtg aaattggcac agcaaaaccc gcttatatct gccagggctg ggggccgcaa
     1141 cgccaggcaa acggcgaact gactgcacgc gctattgcta tgttacctat tttgacgggc
     1201 aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat cgacctatac cattaccata
     1261 gaacgcctgc cggtgctgga taatccggtc aaaacgtcaa tctcctgctt cagctggaca
     1321 gatgctatcg atcatggtcc gcaaatgacg gcaatccgcg acggcgtccg cggcaaagat
     1381 aaactggatg tgcccattaa gttcatctgg aactacgcgg gaaataccct cgttaatcag
     1441 cattctgaca tcaacaaaac gcatgaaatt ctgcaggacg aatcgaaatg cgaaatgatt
     1501 gtgatcatcg aaaactttat gacctcatcg gcaaaatatg ccgacattct gctgccagac
     1561 ctgatgaccg ttgagcagga agatattatt cctaacgact acgccggtaa catgggatat
     1621 ctcattttcc tccagcctgt caccagcgaa aaattcgaac gcaaaccgat ttactggatc
     1681 ctgagtgaag tcgcgaaacg tcttggacct gacgtctatc aaaagttcac agaaggtcgc
     1741 acgcaggaac aatggttaca acatctgtac gccaaaatgc ttgccaaaga tccggcgtta
     1801 ccgtcttacg acgaactgaa aaaatgggta tctataagcg taaagatccc aatggccatt
     1861 ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg
     1921 gtaagattga aatttattcc agcaggctgg cggaaattgc ccgtacctgg gaactggaaa
     1981 aagatgaagt gataagccca ttgccagttt atgcctcaac ctttgaaggc tggaactccc
     2041 ctgaacgtag aaccttccca ctgcaactgt ttggtttcca ttacaaatcc cgtactcact
     2101 cgacctacgg caatattgat ctcctgaagg ctgcctgccg tcaggaggtg tggatcaacc
     2161 ctatagatgc gcagaaacgt gggattgcca acggcgatat ggtgcgggtg tttaaccatc
     2221 gtggcgaagt tcggctacca gccaaagtaa caccacgtat tctccctgga gttagcgcta
     2281 tgggccaggg agcctggcac gaggccaata tgtctggcga taaaatcgac catggcggct
     2341 gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac ccgcagcaca
     2401 ctaatctggt cgagatcgaa aaaatataac ccacgacaac cataaattct ggcatgacat
     2461 tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag gcgctcatga
     2521 aggctgaaat cagccgtaga agtctgatga aaacctccgc acttggcagt cttgcgctgg
     2581 caagcagtgc tttcactctg ccattttccc aaatggtccg ggcggcagag gctccggtag
     2641 aagagaaagc ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt ctgttacgtt
     2701 tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacaggt gacgacgtct
     2761 acggtaacca tcaggttcga gcgtgtttac gcgggcgctc tatccgccga cggatgaatc
     2821 atcctgatag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg
     2881 aacggataag ttgggacgaa gccctggata ccatcagtga taatcttcgg cggatcctga
     2941 aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat ggcggaaaca
     3001 tcaccaactc aaacgtcccg taccgtctga tgaactcttg cggtggtttt ctcagtcgct
     3061 atggcagcta cagtaccgca cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg
     3121 atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc ggaaataacc
     3181 cggcagaaac gcggatgagc ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac
     3241 gttcaaacgc acgcatgatc gtcatcgatc cacgttataa cgacactgct gccgggcgtg
     3301 aagatgaatg gctgcccatt cgccctggca ccgatggcgc actggcctgt gcgattgcct
     3361 gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat tgtgttggtt
     3421 acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa gcctatattc
     3481 tgggcgaagg gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca
     3541 tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa cctgcttata
     3601 tttgtcaggg ttgggggcca caacgacatt ccaacggcga gcaaacgtcc cgcgctattg
     3661 ccatgctttc cgttctcacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg
     3721 aaggtagctg ggatctgggg gtagaatggt tcccgatgct tgagaatcct gttaaaacgc
     3781 agatttccgt ctttacatgg acagatgcca tcgaccatgg tacggaaatg accgcgaccc
     3841 gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg
     3901 ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg
     3961 atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt
     4021 attgcgatat cctgttgccc gacctgatgc caacagagca agaagacctt atctctcatg
     4081 aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg
     4141 aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt
     4201 atcaaacctt tactgaaggt cgcagtcagc atgaatgggt caaatatctc catgcgaaaa
     4261 cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc gggatcttta
     4321 agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg
     4381 caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga
     4441 ttgcagatac ctgggaattg aaaaaagatg aaattattca tccccttcct gcgtataccc
     4501 caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct
     4561 tccattacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct
     4621 gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg
     4681 ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc
     4741 gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg
     4801 gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc
     4861 tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag
     4921 cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc
     4981 tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt
     5041 tatgaatacg ctggcggcga ctggcaggaa gataatggcg tctggcacca gaacgtgttc
     5101 gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc
     5161 cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgatga agatgtttgt
     5221 atcggctgcc gctactgcca catggcttgt ccgtacggcg caccacagta caatgctgaa
     5281 aaagggcaca tgacgaagtg cgatggttgt tattcgcgcg tcgccgaggg gaaacaaccc
     5341 atatgcgtcg aatcctgccc gctgcgtgca cttgagttcg gtccaattga agagctgcgc
     5401 cagaagcacg gcactctggc tgccgtcgcg ccactgccgc gcgcgcattt cacaaaaccc
     5461 aatatcgtta tcaaacctaa cgccaacagc cgcccgaccg gcgataccac tggttatctg
     5521 gctaatccgg aggaagtgta aaatgggaaa tggatggcat gaatggccac tggttatctt
     5581 taccgtattg ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg gctggttcgc
     5641 cgccaaaaat gacgcagacc gtcagcgtat tgtccgtggc atgtttttcc tctggctgtt
     5701 gatgggcatc gggtttattg cctctgtcat gcacctggga tcgccactgc gggcgtttaa
     5761 ctcgctgaat cgaatcggag cttctggtct gagtaacgag attgctgccg gttccatatt
     5821 ttttgccgtt ggcggactat ggtggctggt ggcggtcatc ggtaaaatgc cgcaagcgtt
     5881 ggggaaactc tggttacttg tcagcatggc gctcggcgtc attttcgtct ggatgatgac
     5941 ctgcgtgtat caaatcgaca ctgtgccaac ctggcataac ggttacacta cgctggcttt
     6001 cttcctgacg gtattgttga gcggtccaat acttgctgcc gcaattctgc gggcagcccg
     6061 cgttacattt aataccacac catttgccat cattagcgtt ctggcgctga tcgcttgtgc
     6121 cggggtgatt gtgctgcaag gtttgtctct ggcgtctatt cactcatccg tgcaacaagc
     6181 cagcgcactg gtaccagatt acgcctcctt acaggtctgg cgtgtggtat tgttatgcgc
     6241 aggtcttgga tgctggcttt gcccgctcat tcgtcgccgt gaacctcacg ttgccggact
     6301 tattctgggg ctaatcctca tacttggcgg tgagatgatc gggcgtgtgc tcttttatgg
     6361 cctgcacatg acagtaggaa tggctattgc aggttaacaa attggtgcgg ggcaacccgc
     6421 acatttcagg atgttaggaa tgacccattt ttcacagcaa gataattttt ctgtcgcggc
     6481 gcgcgtgttg ggcgcgctgt tttattacgc tccagagagt gcggaagccg cacctctggt
     6541 tgcggtactc accagtgacg gctgggaaac tcagtggcct ctaccagaag cgtcattagc
     6601 gccgctggtg acggcttttc agacacagtg tgaagagact cacgcccagg cctggcagcg
     6661 tttgtttgtc ggcccgtggg cactgccgtc tccgccgtgg gggtcggtct ggctggatcg
     6721 cgaatctgtg ctgtttggcg attcaacatt ggcacttcgt cagtggatgc gcgagaaagg
     6781 cattcagttt gaaatgaagc aaaacgaacc ggaagatcat tttggttcac tgctgttgat
     6841 ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc gaagaattgc tggcatggca
     6901 cctttttccg tggtcaacac gttttcttga tgtttttatc gaaaaagcag aacacccttt
     6961 ctaccgtgca ctgggtgaac tggctcgctt aacgctggcg caatggcaat cacaactgtt
     7021 aattcctgtc gcggttaaac cgctgtttcg ataggatcac cgtaatattg ccggatggtg
     7081 acgattacgc gcaatccggc aataataggt tacagtgtca cgttttttta tctcttaaag
     7141 cacgcactgc ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt gtttcattgc
     7201 ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat gttccgccgt
     7261 ctgcttatcg caacagtcgt cggtattctc gcggcctttg ccgttgccgg gtttcgtcat
     7321 gcgatgctgc tactggagtg gttgttcctc aataatgact ccggcagtct ggtcaatgca
     7381 gcgacaaacc tttccccctg gcgacggttg ctaactccgg cgctcggcgg actggcggcg
     7441 ggtttgttgc tgatgggctg gcagaaattt acccaacaac gccctcatgc gccgaccgat
     7501 tacatggaag cgttgcaaac cgatggacag ttcgattacg cagcaagcct ggttaaatcg
     7561 cttgcctctc tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt
     7621 cttttagctg cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg ccaggagtgg
     7681 aaattatgga tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg tgccccgctt
     7741 gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc ctctctcggc
     7801 ccggtgatta tttccgccgt cgtggcattg ctggttagca atctgattaa tcatagcgac
     7861 gcgttactct acaacgtaca actctcagtg acggttcagg ctcgtgacta tgcgctgatt
     7921 atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc
     7981 tgtcatcgtg gatttgtgag tctcaaactt gcgccgccct ggcaactggc actaggcggg
     8041 ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc
     8101 gtacaatcct ttttaaccgc cccaccactg ttaatgatca ttgccgggat cttcctttgt
     8161 aaactgtgtg ccgtgctggc gagtagcggt tccggcgcac ccggtggtgt ctttacaccg
     8221 acgctattta tcggtcttgc cattggcatg ttgtatggtc gtagcctggg attatggttc
     8281 cctgatggcg aagaaattac gcttttactc ggattgaccg ggatggcgac actgttggcg
     8341 gcaaccacgc acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac cggggagtat
     8401 cagctactcc ccggtttatt gattgcctgc gtcattgcgt cggtaatttc gcggacgtta
     8461 caccgtgact ctatctaccg ccagcacact gcgcagcata gctaagcgga tgtattgccc
     8521 cagttctcgc tgttcggcgc gcggcagata aggcagttca ccaatgagcg gtgccggaag
     8581 ttttttaccc agcacatcaa tgatttccgc ataatgcgcc agtcctgggt tgattcggtt
     8641 agccacccag ccaatgagcg gcagcccgtc gttggcaatc gcctgagctg ttagcagtgc
     8701 atggttaatg caaccttcct gaataccgac aaccatcaac accggcaact gttcctgcac
     8761 cacccattca gagagtggac gcaaatcatt catcagactg cgccagccgc cagtcccttc
     8821 taccacgaca tgatcgactt tatcggtcag gtttgccagg ccgtttgaaa tgagggtgta
     8881 attgattggg caactgtgcg ccacgctact ttcttcttcg cttaacgcga taggattaac
     8941 tgcttcataa ggcagttcga tggttgaaac actctgcaac accagggcat ctttattacg
     9001 cagcccttcg ggtgtctctt tgctcccctt cgctacgggt ttatatcccg caaccgtttt
     9061 tccctgggag gctaacgctt gtagcaatgc gcgggaaacc accgttttcc ctacagaagt
     9121 gtctgtaccg gtaataaaga aacgcttcag catcactaac tccaccgtta tgcttcacaa
     9181 atataaacca ggaaaataat taaccttgaa agtctaagtt atgctttcct ggcccaaatt
     9241 gagatagcgc aaattttggt agaacagtta aaaaatgtta accctgcaac agacgaatca
     9301 acaaagaacc gttatacatc gcgtctttta ccagtgcagc gcctgccatc gtgccctggt
     9361 tagaaaactg agtactctca acgctgatgt gctgactata cgcaggaagg gcctgctgac
     9421 ggatgctgtc tgagatgacc gggaagagga tatctgccgc tttacttaac ggtgagccaa
     9481 tcagtatttt ttgtgggtta aataaattca ccatgatggc aagaatgcgc cctacatgcg
     9541 cgcccacccc ggtaatgatg tcttttgcca gcagatcgcc gcgcaatgcc gcctgacaca
     9601 atgagtccac ggttaacggt tgtccatgta acatcgagct catggattga ttaagacgca
     9661 gctgtgccag ctcaagaata ctgtccacac tggcgatggt ttcgaggcag ccgtgattcc
     9721 cgcaataaca gcgtttccca tacgggtcga cctgtgtgtg gcctatttcc acgagactac
     9781 tgctgcctgc gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg ttgtgatcga
     9841 taaccacctg aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc tctgccatcg
     9901 tccatgcgct gatatcatgc tgaatataaa ccggtacgcc ggtatgctgc tccagcgcct
     9961 cgccgagcgg catctctttt acatcctcgt agaacggcat gcgatgtaca ataccatttt
    10021 ccgtatcaat aattcccggc aaggttatgg caatcgaagt tagacgctca agttttttct
    10081 ggtggcgaat aaaaaactga tcgatatggg aaatgatacg atccagcaat ggcgagtcat
    10141 cttttaacgc cagttcctgc gactcttcca ccaccagttt gctgctcaga tcgcgcagag
    10201 caaggaaaat ctccccgcga ctaatgcgca gagaaagata gtgccaggct tcagtttcaa
    10261 ccaccagccc caccgccgga cggccacggt tccccgcttc tttgatttcc agctcttgca
    10321 ccaggtgtgc ttcgagcatc tcacggacaa ttttagtgat actggcagga gccagctgcg
    10381 ccagacggga aagatcgata cgcgagactg gaccaagctg atcaatcagg cgataaaccg
    10441 cgcccgcgtt ggtctgcttt atttgatcaa tgtgcccagg ctggttttca gcaaccaccg
    10501 catactccct atattttcgc gctccgaaat aatctgtagg ctatggtgaa gcacttcaat
    10561 acgtgtcgtc aaatttttac ttaggcatgt gattaacagc acatttttcg ggctttttcg
    10621 ctgaaatttc cctcacctga gagcattcag cagatgaata cgaaagttac gcgcagccgg
    10681 actttgttca tgatgtttcg gccagaccaa ccacatttca gaaaccgcat cctcttcagc
    10741 aatcggcacc cagcgcattt cgttgagctg aacacgttta aatgaagcag gcaaaattga
    10801 aacacccaga ccggcggaaa ccagaccgat gatggtcatt gcctcgccca cctcctgagt
    10861 gatgacgggc gtcaaatggt aacgtcgcat cagcccgaga atatcgtcat acagccctgt
    10921 cccgacgtgc ggatcaaaaa agacgaaggg ttctttcgcc agttcagcca gcgttacatt
    10981 cgggttattt gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt catggacgat
    11041 gactgcgtgt tcaagcgtct ccggtaacgc tgtgtaacgc agcaatccca tatccagcgt
    11101 tccttcaatg agcggagcga tttgctcacg agtgttcatt tcgcgggttt gtaaatggac
    11161 atcgggataa tcacggcgaa acagcgataa cgtatcggac accgcccgaa taaaaggagc
    11221 ggacgaagta aaaccaatgc gcaactcccc cgcttccccc tgatgcagtc tttcagcgcg
    11281 agcggcagcg tcatctacca tagacaggat ttgccgacta tctgcaagaa actgttttcc
    11341 tgctgccgtc agcaatacac tgcgattagt tcgtgccagc agtcgggcac caatttgttg
    11401 ctccagtgcc tgaatctgct gacttagcgg tggttgcgaa atattcaggc gggcagcggc
    11461 gcgcccgaaa tgcagctctt ccgcaacagc aacaaagtaa cgcagatgac gaagttcaat
    11521 attcatattt aaaacatctt atttgagatt attaatatat tagacagaac aattcgattt
    11581 tcctaaccta tgtataagcc tgatccacag gcatatttag caaggatttc aagtgagccg
    11641 tactacaact gttgatggcg ctccggcaag cgacactgac aagcaaagca tttctcagcc
    11701 aaatcaattt attaaacgcg gtacgccgca atttatgcgc gtcaccctgg cgctgttctc
    11761 tgccggactg gcaacatttg cacttctcta ttgtgtgcag cctatccttc cggtgctttc
    11821 gcaggagttt ggcttaacgc ccgcgaacag tagtatttca ctgtccattt ccacggcgat
    11881 gttggctatt ggtttgctgt ttactggccc cctatccgat gccattggtc gcaaaccagt
    11941 gatggtcacg gcgctactgt tggcctccat ttgtacgtta ctttcgacaa tgatgaccag
    12001 ctggcacggc attttgatta tgcgcgcctt gattgggctt tcgttaagtg gcgtggcagc
    12061 tgttggcatg acttatctta gcgaggaaat ccaccccagt ttcgtggcct tttcgatggg
    12121 gttgtatatc agcggcaact caattggcgg catgagcgga cgcttaatta gtggtgtctt
    12181 cacggacttt ttcaactggc gaattgctct ggcggcaatc ggttgtttcg cgctagcctc
    12241 ggcgttgatg ttctggaaaa tcctccctga atcacgccat tttcgcccga cttcgctgcg
    12301 acctaaaacg ttgtttatca actttcgtct gcactggcgt gaccggggat taccgttatt
    12361 gttcgcagaa ggctttttgc tgatggggtc gttcgtcacg ctgtttaatt acatcggata
    12421 tcggttgatg ctctccccct ggcatgtcag tcaggccgtg gttggcttat tatcgctggc
    12481 ttatttgacc ggtacatgga gctcacccaa agccggaacc atgaccaccc gctacgggcg
    12541 cggtccggtg atgttgtttt cgacgggggt tatgctgttt ggtttactga tgaccttatt
    12601 cagctcgctg tggctgatct ttgccggaat gttactcttc tcagcaggat tcttcgcagc
    12661 ccactcagtt gccagcagct ggattggccc ccgcgcaaaa cgcgctaaag gccaggcctc
    12721 ctcgctgtat ctgttcagtt actatctggg gtcgagtatt gccgggacgc tgggtggtgt
    12781 tttctggcat aactatggct ggaacggcgt cggcgcattt attgctctga tgctggtcat
    12841 tgctctgctg gtcgggacgc gtttgcatcg tcgtctgcac gcctgaaaaa taagcccgga
    12901 ctgcggtaac tacacgtccg gacttattgc ttgctcaaac caacgttaat agccatccta
    12961 aaatagacga agcgccagcc aattcccgca gcgcgtctag cgtcatcagg attataagta
    13021 cccaaataaa cggattcatt ttgctgtgtg tcatttatta ctgatgcgca gttattctac
    13081 cgctttgtaa gtagtaaaat agttaacccg atcaagacta ctattattgg tagctaaatt
    13141 tcccttaagt cacaatacgt tattatcaac gctgtaattt attcagcgtt tgtacatatc
    13201 gttacacgct gaaaccaacc actcacggaa gtctgccatt cccagggata tagttatttc
    13261 aacggccccg cagtggggtt aaatgaaaaa acaaattgag ggtatgacaa tgaaaaaagt
    13321 attagctctg gttgttgccg ctgctatggg tctgtcttct gccgcctttg ctgcagagac
    13381 tgcgaccaca cctgctccga ctgcgacgac caccaaagca gcgccggcga aaacgacaca
    13441 tcataaaaaa cagcataaag cagcacctgc ccagaaagcg caggcggcta aaaagcatca
    13501 taaaaatacg aaagctgaac agaaagcccc tgaacaaaaa gcgcaggcag cgaagaaaca
    13561 cgccgggaaa cacagccatc agcaaccggc aaaacctgct gcacaacccg cagcgtaagt
    13621 tttcaacagt aatgctggcg cgccccctcg cgcctgaaaa ttacggtgct aagcaggtaa
    13681 cgtttagcac cgcctttagc ggagggtagt atggtgggcc gttatcgctt tgagttcatt
    13741 cttatcatcc ttattttatg cgcactgatt gccgcccgtt tttatctttc ctgactgtag
    13801 ttatctgatt ttactcccac tttcatcccg tcccgtctat agtatttatg agggtttgct
    13861 tttaataatc ataattaccc accagagtgt gatatgcgta caaccattgc tgtagtgttg
    13921 ggtgcaatta gtttgacgtc tgcttttgtg tttgcagata aaccagacgt tgccaaatcg
    13981 gcaaacgatg aggtcagcac cctgtttttt ggtcatgatg atcgtgtgcc agtgaatgac
    14041 acgacccaat caccgtggga tgcggttggg caactggaaa cggccagcgg caatttatgt
    14101 acggcgacgc tgattgcacc caatctggca ttaacggcag gacactgttt attgacgccg
    14161 cctaagggta aagcggataa ggcggtagcg ctgcgttttg tgtcaaataa aggtctttgg
    14221 cgctatgaca tccacgacat agaaggccgc gttgatccga cactgggaaa gcggttaaaa
    14281 gcagatgggg atggttggat tgttcctccc gcagctgcac catgggactt cggcttgatt
    14341 gtgctgcgta atcccccttc tggcattaca ccgttgccgt tatttgaggg agataaagcc
    14401 gcgcttactg ccgcattaaa atcggctggt cgtaaagtga ctcaggcggg atatcctgaa
    14461 gatcatctcg atacgttata cagtcatcaa aactgtgaag tgactggctg ggcgcaaacg
    14521 tcggtgatgt cacatcagtg cgataccttg ccgggtgaca gcggttcgcc tttgatgttg
    14581 cataccgatg acggctggca attaattggg gtgcaaagtt cggctcctgc cgcgaaagat
    14641 cgctggcgcg ccgataaccg ggccatttct gttaccggtt ttcgcgacaa gctggatcaa
    14701 ctgtcgcaaa aataatgttc aaacgctgcc cgacagtgcg ggcagcgtgt tcatcaggca
    14761 agtttcacca tgatcattcc agccaacagc aagaccaggc caatccagcc tttacgattt
    14821 aaccgttgac caaacaagat ccaacctgcg gctaacgtgg cggcaatacc aaacccgccc
    14881 cacaatgcat aagcgacaga caagtcgatc ccttttacgg cctgagaaag cgcactaaag
    14941 gcagccagca ccgccgccag ggagagcaag ccaaatattt tgcgacgaaa gccgtcagaa
    15001 aatttcaaaa agacgttagc aacgatttcc agcacgattg ccaatgccag ccaggcggcg
    15061 tgaacccatt caaactgcgc catggttcac ttccagttca ggtttacgcg ctttacgggt
    15121 acctgatttg atcaacacaa tcccggcgac cagggtggtt aacccggcaa ttttcatcag
    15181 cgataaactt tcgtcgaata acaaaacgct aaacaaggta ataaataaaa taccgatacc
    15241 ttcccacagc gcataagcta cgcctaaggc gatcttttta acggcgaaag agagaaatat
    15301 atacgacaga gaaatcatca ccagcattaa aataaagccg ccatttccct cactgacgct
    15361 cgcccatttc attgacagcg taccggtaat ttctgtagca atagccaggc ctaataaaat
    15421 ccaataaata tacattgtcc ttctcctgca agagaattat tttaattttc gcttaattca
    15481 gcgaaaccaa agtaaagtgg tcaagccccg cgcatagggc aagcttaggc agaagaaagg
    15541 actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat gaggtacgaa
    15601 gatgggtaaa tgttgaaaat aacgtcctga acaaattgtc cataatatta caattatccg
    15661 cagtgttgct tctcgtcatc gcggatgata attgtcctcg gtagttgaac acgcctgatt
    15721 tgtatcatag cttaagaatt aactcaaaat attttcactt ctttacctga gcggtttgat
    15781 tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga aaaaacgggg
    15841 acaagcatgg caaagccgat catcacgctc aatggcctaa aaatcgtcat tatgttggga
    15901 atgctggtca ttattctctg cggtatccgt tttgccgccg agatcatcgt gccgtttatt
    15961 ctcgcattat ttattgctgt tatatataac ttatttcgca taacgcattg atttaattaa
    16021 ataattaaca ccgcaaaaag tgccaaaaat cgcaaaatga ctacaccagt gactacaccg
    16081 ttcggtgcac tgtatgaaac aacgtggaac aaatagacac aagaaatata caggcggatc
    16141 atctttccag ggggaagcac accaattcat gaggggcgtt aatgtcgata tggggatccc
    16201 cacctaccgg aatttttttc cggttaaacg ttaatcaggc tggtgggctt tacctgttcg
    16261 gtaggtgtta tgattatcgt aatacctttc ccccaatggg gtaaaagcat caaccgtaac
    16321 gaaagtcgtt atggttgcca ctgatacccc aaatttgggt atcacgagta agccccaaaa
    16381 tctgggtgtt actcttacta cccaaactac gggtagtttc cgtagtttca tggtcgagtt
    16441 gcagatctgc aactccccca ttaaccacag gcgaatttcc gccttcggtc attccttaat
    16501 catctgcgta atcataatga ttattcagca agcgcaattt tgcgctttgc ttaaagttca
    16561 gcgagttacc gcgccgactt cctcaaattg aggtttccga aaatatcagc gggttagcga
    16621 ttaagctgac cattaattaa actgcgaaaa tgtcgtagtt gtgaggggtg tagccaaccc
    16681 atttttgggc cgtcgggaat atcaaccagt taccgccg
//
LOCUS       DAZFDP010000108        16680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFDP010000108 DAZFDP010000000
VERSION     DAZFDP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHJ1G_003509"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHJ1G_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4765301.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHJ1G_003510"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHJ1G_003510"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4765302.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHJ1G_003511"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHJ1G_003511"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4765303.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHJ1G_003512"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHJ1G_003512"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4765304.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHJ1G_003513"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHJ1G_003513"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4765305.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHJ1G_003514"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHJ1G_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHJ1G_003515"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHJ1G_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4765306.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHJ1G_003516"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHJ1G_003516"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4765307.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10981..11367)
                     /gene="ridA"
                     /locus_tag="ACHJ1G_003517"
     CDS             complement(10981..11367)
                     /gene="ridA"
                     /locus_tag="ACHJ1G_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4765308.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(11440..11901)
                     /gene="pyrI"
                     /locus_tag="ACHJ1G_003518"
     CDS             complement(11440..11901)
                     /gene="pyrI"
                     /locus_tag="ACHJ1G_003518"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4765309.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(11914..12849)
                     /gene="pyrB"
                     /locus_tag="ACHJ1G_003519"
     CDS             complement(11914..12849)
                     /gene="pyrB"
                     /locus_tag="ACHJ1G_003519"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4765310.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(12853..12987)
                     /gene="pyrL"
                     /locus_tag="ACHJ1G_003520"
     CDS             complement(12853..12987)
                     /gene="pyrL"
                     /locus_tag="ACHJ1G_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4765311.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(13268..13663)
                     /gene="yjgH"
                     /locus_tag="ACHJ1G_003521"
     CDS             complement(13268..13663)
                     /gene="yjgH"
                     /locus_tag="ACHJ1G_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4765312.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(13794..14507)
                     /gene="bdcA"
                     /locus_tag="ACHJ1G_003522"
     CDS             complement(13794..14507)
                     /gene="bdcA"
                     /locus_tag="ACHJ1G_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4765313.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            14578..15171
                     /gene="bdcR"
                     /locus_tag="ACHJ1G_003523"
     CDS             14578..15171
                     /gene="bdcR"
                     /locus_tag="ACHJ1G_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4765314.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            15316..15768
                     /gene="tabA"
                     /locus_tag="ACHJ1G_003524"
     CDS             15316..15768
                     /gene="tabA"
                     /locus_tag="ACHJ1G_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4765315.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            15891..>16680
                     /locus_tag="ACHJ1G_003525"
     CDS             15891..>16680
                     /locus_tag="ACHJ1G_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765316.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDE"
BASE COUNT         4050 a         4136 c         4318 g         4176 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcgttttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatcc tgataagcgt agcgcatcag
    10921 gcaattttgc gtttgtcatc agatttaatc cgggcatgat agcccggatt tccatcaaga
    10981 ttagcgacga acagcgatcg cttcgatctc aatttttacg tctttcggca gacgggcaac
    11041 ttcaacgcaa gaacgtgccg ggaaggtggc attgtgttcg gtgaagaagg cttcgtaggt
    11101 agcattaacg gttgcgaagt cgttcagatc tttcacaaac acggtggttt taacgatgtc
    11161 gcccactttc aggcctgcgg cttcgacgat cgcttttacg ttatccagcg actgacgtgc
    11221 ctgtgcagcg acgtctgccg gtacttcgcc cgttttcgga tttaccggga tctggccgga
    11281 ggtgatgatc atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc
    11341 attttccgtc gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg
    11401 gctcattata gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt
    11461 atgggaaaac tctttttcac agtatttgca tttgagcgcg atgtcattgg cgcgttttcg
    11521 cacggcaaag ctggaagaaa ccggttcggc atggctgata cagttgctgt tcgggcagac
    11581 cagcacattg tcgatgcgct ccggcagact ggggcgcgat ttacccacca cttcatagtt
    11641 gtcgatacgg ttaaccgtgg cttgcggcgc atacaatgcc agttgatcta cctgatcttc
    11701 actcaaaaag gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcag
    11761 gttcagacca atggtgatgc gctgatccgt ttcggtcagc ttgaacagac tcaacagctt
    11821 aaaaccgatc tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg
    11881 caatttatta tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac
    11941 cagtgccagt aacgcctggc gagcgaaaat cccgttgcct gcctgctgga agtaccaggc
    12001 gtgtggcgtt ttatcaacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac
    12061 tttcatattg gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac
    12121 gttggcgtac tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc
    12181 tacttccgcc atcacttctt caatagagct gtgcagactc catgcgatcc ctttttcatc
    12241 gagcatatcc agaatgtatt gcggcattgc cagcgcgtcc ggcgcgatga agtaaaaacg
    12301 gttgccatcg aacttcgcta acgcctgagt cagggagtga acggtgcggc catatttcag
    12361 gtcaccaacc attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatagtgaa
    12421 taagtccagc aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg
    12481 tacattgccg gaaaactcgg tggccaggcg cgccgcacct tcctgcggat gacgcatcac
    12541 tatcgcatcg acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcccttttt
    12601 acccagtgat gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat
    12661 agatgtttcg aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac
    12721 tttgtgcttc aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac
    12781 cagattaagg tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg
    12841 attagccatc ttttatctcc tgacgcctgg gcaaaaaaaa gcccctcgat tgaggggctg
    12901 ggaatgggtg atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa
    12961 gacaaaatgt cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact
    13021 catagccaga agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt
    13081 gaattaaaag tcaaaatgta tgcattttta tttgctttga ttctggagga gaactgtcgt
    13141 tcggtatcaa taattgcaga catttcctgc ttttttcttt ttcactttca cgcaatcaga
    13201 tatgcatttt attccattca tccgacttat agggcggagt gtgaaaacga acggctaaca
    13261 ctattgctta cagctcaggg atgcgcgcta tcactttaat ttcaaaatca aagcctgcca
    13321 gccaggtaac gcccaccgcc gtccagtttg gataaggagg ggcgctaaat atttcatttt
    13381 tgaccgtcat gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa
    13441 tgatatcgtc aaaagtgcat cccgcagcag ccagggtcgc atgcaaatta tcaaatgcca
    13501 gtctgacttg ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc
    13561 cggaaacaaa cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca
    13621 gtgaatgtcg gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat
    13681 ttacgacatg aacagaagat ttctctttac cgggagccgc ttttagcgga cgacgtgagt
    13741 aaacaaaacc cagacatcat ggataatggc tgggcttaat tgagcgtagt cggttatgcg
    13801 ccaaacgcgc catcaatggt atgcatcgcg ccggtaacaa aactggcttc tggccctgct
    13861 aaccatgcga ccataccagc gacctcttcc ggttgcccat gtcttttgat agccatcaaa
    13921 ctatgcaaca tatcgcgcat tggcccgttg gcgggattag cgtcggtatc aattggccct
    13981 ggctggacga cgttaatggt gatcccacgc ggtccaaaat cacgggccag cccgcgcgcc
    14041 atgccttgca gggcagattt gctggcggca taagcagcca tgcctgcaac aggcatacga
    14101 tcgccattca cggagccgat gattaagatg cgcccgcctt cgggcatctg ccgggcggct
    14161 tcaacagagg catgataagg agcatgaata ttgattttga aaaggcgatc aatatcgtcg
    14221 gcatttaatt ccagggcatc gccaaagacg ccaatacctg catttaccac caggatatcc
    14281 aatgcgccgc tcttacgaac gacatcaatg acagcgtctc tgtcagcact atctgtaaat
    14341 actgctgtcg ctccagtctc ttgtgccaga tgttcagcgg catctttcga ccccgcatag
    14401 gtgaatcgta cattggcccc atcagtgacg aaacgacgta cgatagcggc accgattcca
    14461 cgactgccac cgaggatgag aactgtctta cctgtaaaag cgcccataag gactccttga
    14521 tttattatgt aacatgcatt acaaaactgt tttaactttc tgtcaacaag ttttgtaatg
    14581 gtcactaaaa aacaatctcg cgttccaggt cgtcccagac gtttcgctcc tgagcaggca
    14641 gtctctgcgg caaaagtgct ttttcaccaa aaaggtttcg atgctgtcag tgttgctgaa
    14701 gttactgatt atcttggtat taaccccccg agcctctacg cagcttttgg cagtaaagtt
    14761 gggttattta gccgtgtact caatgaatat gtcggtacgg aagctattcc gcttgccgat
    14821 attcttcgtg atgatcgtcc ggtaggcgag tgcctggttg aggtattaaa agaagcggcg
    14881 cgcagatata gccaaaacgg cggctgcgct ggctgtatgg ttcttgaagg tattcatagt
    14941 catgatccac aagcgcgtga tattgccgtt caatattatc acgccgcaga aacgaccatt
    15001 tatgactata tcgccaggcg gcatccacaa agcgcacaat gtgtgactga ttttatgagt
    15061 accgtgatgt cagggctttc tgcgaaggca cgggaggggc actccatcga acaactctgt
    15121 gcaacagctg cactggcggg ggaagcgata aaaactattc tcaaggagtg atgctggcct
    15181 tgacccgaaa ggcgggaacg cgcctgccga taagttgtga taagacaata attcacgcgt
    15241 taaggctagc ggaattgatc atcttttcgt ataacggtgg aaatgaaaca ttgttttaat
    15301 taaggagtgg aaaagatgat catcggaaat attcataatc ttcagccgtg gctaccccag
    15361 gagttacgcc aggcgattga gcatatcaaa gcgcacgtta cggcagaaac gccaaagggt
    15421 aagcacgata tcgaaggcaa tcgtctgttt tatcttatct cggaagatat gaccgagccg
    15481 tacgaagctc gccgtgcgga gtatcatgcc cgctatctcg acattcagat tgtgttaaaa
    15541 ggtcaggaag gcatgacctt cagcacgcaa cctgcaggca cgccggatac cgactggtta
    15601 gctgataaag acatcgcatt tttgccggaa ggcgttgatg agaaaacagt tatccttaat
    15661 gaaggtgatt ttgttgtgtt ttatccgggg gaagtgcata aaccgctgtg cgcagtgggc
    15721 gcgccagccc aggttcgcaa agcagtagtg aagatgctga tggcgtgatg acttttcgcc
    15781 gtaaataact ccaggtttac ggcgagtttg tgaaaagagc gttttttgat atttttttgt
    15841 gagtagaatt tgtaatgctt agacgttctt attaactcaa ggagtccgtc atggcaaaaa
    15901 tatcagattt gaattgttat caacacatgg catcagccga caatgttaaa ccaaaaaatg
    15961 aagtcttaaa tacctggtgt gtgggaatga atgattttgc tcgtaatgcc gaggggcagg
    16021 atagcagaag gaatattcta tctcctggag catttttgga gtttttggca aaaatattta
    16081 ccctgggtta tgtggatttt agcaaacgct ccaacgaagc gggtagaaat atgatggctc
    16141 atattgagtc ctcatcttat atcaaaaata atgatggcag tgaaataatg aattttgtta
    16201 tgaataatcc tgaaggtgag cgtgcggatt catctaaggt gattatagaa atttcacttt
    16261 ctactaccac tactatgggg actcgtcaag gacatacagc cattatattt ccacaatctg
    16321 attttttgag ttttcgttat gaaggtaagt cttttgaaag aaaagatgat agttcattac
    16381 atctgattac taacaaggtt ctggcgtgtt accaacgcga agctaacaag aatatggcgc
    16441 ttctattaaa taatcatcag ttgaactgga aggacgcaat tgattataac gagttattag
    16501 aaaagccatt agccagtaca ttagaaaaaa taaagaaaga acatctgcta ttaatgcctc
    16561 atgtatgtga tgacacgatt ccatacttac taggcgaagg tggtatactt gaagaaataa
    16621 atgatctgaa agcgttacat gataccagaa ttgctagtgg tacagaaggt aatgatgaaa
//
LOCUS       DAZFDP010000109        16495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFDP010000109 DAZFDP010000000
VERSION     DAZFDP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..177
                     /locus_tag="ACHJ1G_003526"
     ncRNA           46..177
                     /ncRNA_class="other"
                     /locus_tag="ACHJ1G_003526"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(361..1038)
                     /gene="narI"
                     /locus_tag="ACHJ1G_003527"
     CDS             complement(361..1038)
                     /gene="narI"
                     /locus_tag="ACHJ1G_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4765317.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1038..1748)
                     /gene="narJ"
                     /locus_tag="ACHJ1G_003528"
     CDS             complement(1038..1748)
                     /gene="narJ"
                     /locus_tag="ACHJ1G_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4765318.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1745..3283)
                     /gene="narH"
                     /locus_tag="ACHJ1G_003529"
     CDS             complement(1745..3283)
                     /gene="narH"
                     /locus_tag="ACHJ1G_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4765319.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3280..7023)
                     /locus_tag="ACHJ1G_003530"
     CDS             complement(3280..7023)
                     /locus_tag="ACHJ1G_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4765320.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKISNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7538..8929)
                     /gene="narK"
                     /locus_tag="ACHJ1G_003531"
     CDS             complement(7538..8929)
                     /gene="narK"
                     /locus_tag="ACHJ1G_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4765321.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9268..11064
                     /gene="narX"
                     /locus_tag="ACHJ1G_003532"
     CDS             9268..11064
                     /gene="narX"
                     /locus_tag="ACHJ1G_003532"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4765322.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            11057..11707
                     /gene="narL"
                     /locus_tag="ACHJ1G_003533"
     CDS             11057..11707
                     /gene="narL"
                     /locus_tag="ACHJ1G_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4765323.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11708..13102)
                     /gene="ychO"
                     /locus_tag="ACHJ1G_003534"
                     /gene_synonym="iatA"
     CDS             complement(11708..13102)
                     /gene="ychO"
                     /locus_tag="ACHJ1G_003534"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4765324.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13287..13640
                     /gene="ychN"
                     /locus_tag="ACHJ1G_003535"
     CDS             13287..13640
                     /gene="ychN"
                     /locus_tag="ACHJ1G_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4765325.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13684..14379)
                     /gene="chaC"
                     /locus_tag="ACHJ1G_003536"
     CDS             complement(13684..14379)
                     /gene="chaC"
                     /locus_tag="ACHJ1G_003536"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4765326.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14537..14767)
                     /gene="chaB"
                     /locus_tag="ACHJ1G_003537"
     CDS             complement(14537..14767)
                     /gene="chaB"
                     /locus_tag="ACHJ1G_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4765327.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15037..16137
                     /gene="chaA"
                     /locus_tag="ACHJ1G_003538"
     CDS             15037..16137
                     /gene="chaA"
                     /locus_tag="ACHJ1G_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4765328.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         4001 a         4411 c         4133 g         3950 t
ORIGIN      
        1 acttttcaaa agtccgtaaa ctctcaaacg aatccgcaat taaatattct gcccaagcgg
       61 ggaaggatga gaagcttcga ccaaggttcg acttgagcgc cagcgagaga gcgttgccgc
      121 aggcaacgac ccgaagggcg aagcgcgcag cgttgagtaa tccttccccc accaccatca
      181 cttccttaaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
      241 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      301 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc
      361 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      421 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      481 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      541 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      601 gaacggaatg gtcaacaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      661 atccgctccg gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag
      721 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      781 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa
      841 gtgaccaaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      901 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      961 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
     1021 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
     1081 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
     1141 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1201 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1261 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1321 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1381 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1441 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1501 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1561 ctgtactggg cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1621 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1681 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1741 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1801 tgaacagatt gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc
     1861 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1921 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1981 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt
     2041 aatgacgcat cgccaacata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg
     2101 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaatgc
     2161 cgttgctacc cagctcaccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2221 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
     2281 ttgccatttt ataaaccggc gactgctgtg cggcttcaat aacgctcagc ggaataccgt
     2341 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacc tccagctgac
     2401 gctggtaaag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2461 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2521 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2581 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2641 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2701 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2761 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2821 gtttgtcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2881 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2941 gcgcggtatg caggttctga tagtcaaaat cgaacggctc gtaataatcg tcgatccccg
     3001 gcagatgcgg gttagcgaag attttaccca gcaacatggc acggttaccc atgcgcggct
     3061 gcagtttgcc gttgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     3121 cagtcgggaa gccctgaccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3181 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3241 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3301 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3361 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3421 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3481 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3541 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gagcaccgtt
     3601 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3661 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3721 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3781 gccattggat ttctggccca tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3841 aaccagcagg ctttcaccga aatcacgcat ccattggtga tcctgataca gctgctgacg
     3901 gccagagagc gtacgccacg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3961 aacgtgttca tcttcaaggc cagaccaggt cgggctggag atgattttgc gcggctgtgc
     4021 ctgaatatcg cggaagcgaa tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     4081 acgaccggta aactcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     4141 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4201 tttcgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4261 ggtgttccag gcaatccctt taccgccatt actgattttc tccatcagcg ggccgataga
     4321 ggtaaagcgt tcgtaagtcg ccggataatc gcgctctacg accataatgt gtggcgcggt
     4381 tttaccaggg atcaggtcgc actcgccttt tttccagtct ttcacatcca gcggctgcgc
     4441 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4501 atgaccgacg cacacttcgg agaatttctt cgcgatggct ttgtaaattt cccagtcgct
     4561 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4621 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4681 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4741 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4801 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4861 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4921 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4981 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     5041 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     5101 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     5161 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5221 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5281 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5341 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5401 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5461 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5521 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5581 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5641 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5701 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5761 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5821 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5881 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5941 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     6001 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     6061 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     6121 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6181 gacggcaaca gttttggtcc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6241 acgcgtctgc ggtacgtttg acccccaggc gatgatgtag ctggagttgt accagtcagc
     6301 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaagtcgca
     6361 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6421 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6481 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccagga
     6541 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6601 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6661 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6721 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6781 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6841 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6901 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6961 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     7021 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     7081 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaagt gtgatgggga
     7141 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt aaagcataac
     7201 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa agcatgaaga tttttatgca
     7261 gatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7321 cgaataagcc atataacatt agtatgttgt aactaaataa cgcataaaaa aagcgcgaca
     7381 tcatgccgcg caaaggataa tcaaatattt tactctatat aattcggttt acaggaaggc
     7441 ggcaagtgaa tgaatcccag gagcttacta aagtaagtga ctggggtgaa tgaacgcagc
     7501 cgcagcacat gtaacccgaa gtatgacgag taacggttta ttttttagaa tgccgaccat
     7561 ataccgccca ggtaatcact acgcaggcga tatagaaaat caaaaatacc ttcattgcgc
     7621 cgactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata aagaagccac
     7681 caatagcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgtcggtt gccgcttcac
     7741 gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc agtttacgga
     7801 agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc aggaacagcg
     7861 ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc ggtaaggtca
     7921 ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg acacgagttc
     7981 cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca ataaacggcc
     8041 cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac agcattgcaa
     8101 agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg ctcataatcc
     8161 acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc gaggtagcaa
     8221 gatcgttcat gccaaaccac gccgcaatag tgaagatggc aaggaacggc acccatatcc
     8281 aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc tggctaccaa
     8341 atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg acgctgacgc
     8401 ccatgttgcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt ttcggaaaga
     8461 agaagctgat gtttgccata ctggaggcga agttcgcgcc agcaaagccg catagcagag
     8521 agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa cccagccaga
     8581 cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca ccgaagatag
     8641 gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc agcgcagtca
     8701 acataaatag ctgatcggtc gtaaaattaa agccgacttt cggtaggttc accgcaacag
     8761 cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa atccacaggt
     8821 tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc ggtcgccaat
     8881 ctgtaatgac agctccagta gccctttcgg gggcggatga gtgactcata gacacctctg
     8941 atactcgttt cgatgtctgc caccttagtg tctgtagcta aaggcaattt gatgtaaatc
     9001 aaacgataag ataactttat ctatcattga tatttatcat tacccatagt gagtacagtg
     9061 atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct taacgatgta
     9121 aagcgactaa ccacacggca aataaggagt aactctttcc aggtatgggt atacttcagc
     9181 caatagccga gaatactgcc attccagaat gtatcgtcac attcattaag gttattgctc
     9241 atttaaagcc tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc gctcaccctg
     9301 gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc agggatggcg
     9361 gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa caaagcggga
     9421 tcgctgcgca tgcaaagtta ccgtctgttg gcagcagtgc cattaagcga gaaagacaag
     9481 cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg agcagcagaa
     9541 cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa tgaactgatc
     9601 cctgcgttga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag ccagtttgtt
     9661 gccgggcttg atcaactggt atctggtttt gaccgcacca cggaaatgcg catcgagaca
     9721 gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt gttcactatt
     9781 atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat ggcgagtgcc
     9841 gtcagtcatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga aatggcgatg
     9901 cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc cgtacttgag
     9961 cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct ctctttttta
    10021 tggcaggcta accgccgttt gcattcccaa gccccgctgt gtgaacgcct gtcacctgta
    10081 ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt ttatgatact
    10141 gatgatgaag agaatcatca ggagtttacc tgccaaccag atatgacttg tgatgataaa
    10201 ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac aaccctgaag
    10261 tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct gccgcagggg
    10321 cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca actcaccgcc
    10381 acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat ggaagagcgt
    10441 gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg catgaagatg
    10501 caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg cgaactgtta
    10561 agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt gctcaccaca
    10621 ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaggcgag ttgcgaagag
    10681 tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc tcgcctggtg
    10741 ccttcgcatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag taacgccctc
    10801 aaacactcgc aagcgagtga agtcgtggtg acggtggcgc aaaacgataa tcaggtcaaa
    10861 ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag caatcactac
    10921 ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg cgtccgccgt
    10981 cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac tttcacagac
    11041 gtccaaggag atacccatga gtaatcagga accggctact atcctgctga ttgacgatca
    11101 cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata tcaccgtggt
    11161 tggcgaagcg agtaatggcg aacagggtat tgaactggcg gagtctcttg atcccgatct
    11221 gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg ataaactgcg
    11281 cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg aagaagatgt
    11341 ggtcaccgca ctgaaacgcg gcgcggatgg ttatctgtta aaagatatgg aaccggaaga
    11401 tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg aagcattaac
    11461 gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg atgttaacca
    11521 gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc cgaacaagat
    11581 gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct
    11641 gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat
    11701 tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac ggttcgacaa
    11761 gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc accaccaccg
    11821 acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg atcagcgtcc
    11881 agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc gtatcgccct
    11941 gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc ggcactgttt
    12001 cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt tttcgctgtc
    12061 ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta ccacgtaacg
    12121 actgactttc ggcaacctca cccgcagaaa gttgtttttt gagcggtaca ccaaagcggt
    12181 aattaagatt cagcccgagg ttattttgat tctcgccact ttcaccctgt ttatgctggg
    12241 ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac gcgacgggat
    12301 tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac tgttctacgc
    12361 tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgggctgtc aggtcatacc
    12421 cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca aacggctgat
    12481 aaaagtttgc cgatagtcgc aaatattcgc cccacgcttc ggcaccaaag cccgctcgct
    12541 gaagattttc gtccagcaag ttgtcataaa aagtgttata acccaccagc cagttgccgc
    12601 gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc atcatcctgc tgagtaagac
    12661 caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac cagcttccac
    12721 gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca tttccccacg
    12781 gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg cggacttttc
    12841 ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc atactggttt
    12901 cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt tcgggagcca
    12961 tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct ttttgctcga
    13021 aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa aacgggataa
    13081 tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa aaaccatagc
    13141 cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc
    13201 cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa gatgaatgtc
    13261 taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac
    13321 cttacgggag cgaatccttg tttaacagct tgcggctagc cattgcgtta cgagagcagg
    13381 agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg
    13441 ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt accgctcaga
    13501 atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagt acacttcctc
    13561 tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg ctgtcagccg
    13621 ataaagtgct cacattttaa tgtttgaggg ggcgtctgac gccccactat gagggaagtt
    13681 tactcaggcg aatcccggcc gtaacacacc atccggataa ttctccgcca gcagtttttt
    13741 taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga taagctcctg
    13801 ttccagtgaa aacaggtatt gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa
    13861 cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg ggtccataat
    13921 aaacacaatg gcgtttactg tgcatccatc atcaagatca agctgacacc aggttggcag
    13981 ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct ccagcgtctc
    14041 ttctggcagt cgataggcga cgcctgtagt gcgtccgccc tctttcagtg caagcatccg
    14101 tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca
    14161 tccaaccagt gtaccggtgc acgactcggt aaactccagt gccggattcc acatcaacga
    14221 tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg tcgccgccag
    14281 cgaagccgcc cgttgctctg ctgaccataa gagtgattct tcaatggcac caaatgccgt
    14341 tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc gccactgcat
    14401 acttccctgc gacaactttt tggtgtcttc cggacatttt tactgctcat tttgaaacca
    14461 ctaacgggac aatatgcaat ccacggacca cttgcaaggc aacaccgcac gaatagcttt
    14521 aactaagcta ccggttttac gattttttat gccatttatc atcatcccct ttggcatatt
    14581 catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg gcgtcatcac
    14641 gccgatcgtc tttatcttta tattgatccc atgcgctgtt gaacgcttct ttatagatat
    14701 cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt ttcgttttat
    14761 acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca atatctactc
    14821 ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa aacaaacatt
    14881 gtctaaacgt aatacttatt tttatgtccc aatgagtaaa aaatcgacag tggggtaaaa
    14941 attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc tattttttac
    15001 attttcgata aattcatctg caaagggata accataatgt caaatgctca agaggcggta
    15061 aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggcgctggt agtgctgttc
    15121 ctgtggggaa gcagccagac actaccagtg gtcattgcca tcaatcttct tgcgcttatt
    15181 ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg
    15241 ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga agtcagtttg
    15301 atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga tacgctctat
    15361 tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt gggcggtcgt
    15421 aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat tgcgctgttc
    15481 cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa tttttcaacc
    15541 ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt tttgctgatc
    15601 cagaccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag tgatgacgat
    15661 gacccgcatc acggtaaacc gtctgcccat agcagcgtgt ggcatgctat ctggttgatt
    15721 atccatctga ttgccgttat tgcagtcacc aaaatgaatg ccagcccgct ggagacattg
    15781 ctcgacagca tgaatgcccc tgtcgccttt actggcttcc tggtggctct gttgattctg
    15841 tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggtcca gcgcgcgatg
    15901 aatctgttct ttggttcagt gttagcaacc atttcgctta ccgtacctgt cgtcacgcta
    15961 attgccttta tgacgggtaa cgaattacag tttgcacttg gtgcgccaga aatggtggtg
    16021 atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac taacgtgctc
    16081 aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt tgcctgacag
    16141 ttatatctcc tccgatgacg gaaatcgggg gagataagat aaattcctgc ttattgtatt
    16201 tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca caacctgcac
    16261 gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa acatttcagg
    16321 gatgaagtga agatgaattg cgacgtagta tccacaccaa gccggcgtta tccggtgagg
    16381 cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag aaaacccccg
    16441 cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc agaca
//
LOCUS       DAZFDP010000110        16111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFDP010000110 DAZFDP010000000
VERSION     DAZFDP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..1299
                     /gene="yghO"
                     /locus_tag="ACHJ1G_003539"
     CDS             157..1299
                     /gene="yghO"
                     /locus_tag="ACHJ1G_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO4765329.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1306..2070)
                     /gene="glcC"
                     /locus_tag="ACHJ1G_003540"
     CDS             complement(1306..2070)
                     /gene="glcC"
                     /locus_tag="ACHJ1G_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO4765330.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2321..3820
                     /gene="glcD"
                     /locus_tag="ACHJ1G_003541"
     CDS             2321..3820
                     /gene="glcD"
                     /locus_tag="ACHJ1G_003541"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO4765331.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3820..4872
                     /gene="glcE"
                     /locus_tag="ACHJ1G_003542"
     CDS             3820..4872
                     /gene="glcE"
                     /locus_tag="ACHJ1G_003542"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO4765332.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4883..6106
                     /gene="glcF"
                     /locus_tag="ACHJ1G_003543"
     CDS             4883..6106
                     /gene="glcF"
                     /locus_tag="ACHJ1G_003543"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO4765333.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6111..6515
                     /gene="glcG"
                     /locus_tag="ACHJ1G_003544"
     CDS             6111..6515
                     /gene="glcG"
                     /locus_tag="ACHJ1G_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO4765334.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6537..8708
                     /gene="glcB"
                     /locus_tag="ACHJ1G_003545"
     CDS             6537..8708
                     /gene="glcB"
                     /locus_tag="ACHJ1G_003545"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO4765335.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8936..9181
                     /gene="traT"
                     /locus_tag="ACHJ1G_003546"
                     /pseudo
     CDS             8936..9181
                     /gene="traT"
                     /locus_tag="ACHJ1G_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9214..10956)
                     /gene="cptA"
                     /locus_tag="ACHJ1G_003547"
     CDS             complement(9214..10956)
                     /gene="cptA"
                     /locus_tag="ACHJ1G_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4765336.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            11413..12626
                     /locus_tag="ACHJ1G_003548"
     CDS             join(11413..11704,11704..12626)
                     /locus_tag="ACHJ1G_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4765337.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGGRVSSS
                     METTFVLDALEQALWARRPSGTIHYSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    11694..11810
                     /locus_tag="ACHJ1G_003548"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(12693..13457)
                     /locus_tag="ACHJ1G_003549"
                     /pseudo
     CDS             complement(12693..13457)
                     /locus_tag="ACHJ1G_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            13677..14034
                     /locus_tag="ACHJ1G_003550"
                     /pseudo
     CDS             13677..14034
                     /locus_tag="ACHJ1G_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14288..>16111)
                     /gene="iha"
                     /locus_tag="ACHJ1G_003551"
     CDS             complement(14288..>16111)
                     /gene="iha"
                     /locus_tag="ACHJ1G_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4765338.1"
                     /translation="GMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAATRIPYPTESQNYNLGARLDWKASEQD
                     VLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKSYLNWN
                     ETENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGESHLVTVGGEYQSS
                     SMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHEQFGGHFSPRAYL
                     VWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPDLKPEESVSYEAG
                     VYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHGVEFAGTLPLWSE
                     DVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTP
                     RFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNAAVNNLLNKDYSD
                     VSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT         3987 a         3961 c         4322 g         3841 t
ORIGIN      
        1 ggcattgagg cgcttctgtg tagtctctaa ccttatcata atcattaata aatactattt
       61 attctttgtt tgtattcatt gggttgatta ttaaggtgtc gttcgttgct gttgcaacct
      121 gtttttcacc gttaattatt tgttatccac agagatatgg aatgcgatct gctgatgatt
      181 aaaattgaaa aagtaattaa taaaaatgac cttaaggcat ttatcgcttt tccgtcatca
      241 ctttatcccg acgatccaaa ctggataccc cctttattca tagaacgcaa cgagcatttg
      301 tctgcgaaaa atccggggac ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg
      361 gggcagatag ttgggcgtat taccgcgcaa atagatacct tgcaccgtga gcgttacggt
      421 gaagataccg gtcatttcgg catgattgac gccattgatg atccgcaggt ttttgccgcg
      481 ttgtttggcg cagcggaagc gtggttgaag tcacaaggtg caagtaagat cagcggtcct
      541 ttcagcctga atatcaatca ggaaagcgga ttactgattg aaggttttga cacaccaccc
      601 tgtgcgatga tgccacacgg caaaccgtgg tatgccgcgc atattgaaca actgggttat
      661 cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg
      721 gcgctaaaaa aactgatgga tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt
      781 cagcggtttg ccgaagaaat gcagatcctg cgtgagattt tcaactctgg ctggcagcac
      841 aactggggat ttgtgccgtt taccgaacat gaattcgcga ccatgggcga tcaacttaaa
      901 tatctggtgc cggacgatat gatctatatc gctgagattg attctgcacc ctgcgcgttt
      961 attgtcggct tgccgaacat caacgaggcg attgccgatc tgaacggatc gctctttccc
     1021 ttcggctggg caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac tgcgcgagta
     1081 ccgctgatgg gcgtgcgcga cgagtatcag ttcagccgca tcggcccggt gattgcgctg
     1141 ttattgattg aagccttacg cgatccgttt gcccgccgga agatcgatgc gctggagatg
     1201 tcatggatcc tcgaaaccaa tacaggcatg aataacatgc tggaacgcat tggggcggag
     1261 ccgtacaagc gttaccgatt gtatgaaaag cagatttaat accagctaac tcaggttcat
     1321 ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt
     1381 ctttttcacg gtccgcacat gatcgcgtgc tgcgcgctgg gcgacgtgcg gcagccgctg
     1441 caacaccgcg ttgtagatcc tcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg
     1501 atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg
     1561 cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg
     1621 atccaactgc gcatgttcga tcagcgaaat ctctttgttg ttctcactgg cggcgagcat
     1681 tttttcataa cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag
     1741 ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg
     1801 ctgcgtactg aacagatgga tcagcggact ggtgtcctgc acccgattaa gccgtgcgac
     1861 acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc
     1921 ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag
     1981 tggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac
     2041 ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat
     2101 ttttctgttc ttaactcgca aaacacgcac atcacgtaag tgattatgtt acatcaattt
     2161 aacattgagt taaccaaggc aaggtcacag agctggaaaa aaaatggtct gaccggtagc
     2221 taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa
     2281 aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2341 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2401 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2461 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2521 ggtgacagcg attcttgctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2581 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2641 gcgctttaaa gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg
     2701 cgtgcgtaac ctggcgatct cccaggccgt tgcaccgcat aatctctact acgcaccgga
     2761 cccttcctca caaatcgcct gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt
     2821 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     2881 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     2941 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     3001 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     3061 cggacttgca gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     3121 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     3181 cgaagcgatt ttgttatgcg agctggacgg cgtggagtct gacgtacagg aagactgcga
     3241 gcgggttaac gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg cacaggacga
     3301 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3361 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3421 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3481 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc
     3541 ccgcgcggaa gagctgggtg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3601 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3661 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3721 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3781 tcacggtcat ttacctttcc ctgaactgga gcgtttctga tgctacgcga gtgtgattac
     3841 agccaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     3901 cagggcagca atagcaaagc ctttttaggt cgccctgtca ccgggcaaac gctggatgtg
     3961 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgcgcgtgcc
     4021 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc
     4081 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     4141 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     4201 accggcgctg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4261 gatctctcac ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg
     4321 atgaaagtgt taccgcgacc gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa
     4381 gaagccatta acgaaatcgc ccagtggcaa ctccagccgc tacccattag tggcttatgt
     4441 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcggcg
     4501 cgtgaactgc tggggggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa
     4561 caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg
     4621 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggagcgtt acgctggctg
     4681 aaatcgacag ccgaggacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4741 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4801 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     4861 gcggaacttt gaggagcagg ctatgcaaac ccaattaact gaagagatgc ggcagaacgc
     4921 gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc
     4981 aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta
     5041 tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga
     5101 tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa
     5161 tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg
     5221 aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac
     5281 gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc
     5341 tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct
     5401 ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct
     5461 ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga
     5521 ctatcatctt aatgcgcagg agaaagggct ggcacgggcg cgcaataata ttgatgcctg
     5581 gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc
     5641 gtttgtcaaa gagtacgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg
     5701 tcaagtcagt gaactggcgg tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa
     5761 actggcagtt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc
     5821 gcaaaagctg aacggcgaag tggaaaaagt gttgcttcgt cttggattta ccttaacgga
     5881 cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga
     5941 tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat
     6001 gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctcagt
     6061 gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta
     6121 aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc
     6181 agaaaaataa ctggtcggtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt
     6241 taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta
     6301 ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta
     6361 ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgtgg
     6421 atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg
     6481 tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga
     6541 gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg
     6601 aagaagtttt accgggaaca gggctggacg ctgcggcgtt ctggcgcaat tttgatgaga
     6661 tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg
     6721 cagcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata
     6781 aatctttcct gcgtgaactg ggctacctgg tgccgcaacc ggagtgcgtg acggtggaaa
     6841 ccacgggcat tgacagcgaa atcaccagtc aggcagggcc acagctggtg gttccggcaa
     6901 tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt
     6961 tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagcggctac gatccgcagc
     7021 gcggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatctcta ccgctggaaa
     7081 acggcagcta tcaggatgtg gtggcgtttc aggtggtcga taaacaatta cgcatccagt
     7141 tgaaaaatgg taaagaaacc acgttacgta ccccggcgca gtttgtcggt taccgtggcg
     7201 atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa
     7261 ttgatgccaa cggacggatt gggaaagacg atgcggcgca catcaacgat gttatcgtcg
     7321 aagctgcaat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag
     7381 ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctc caagagaaaa
     7441 tggagaaaaa cggtcgacaa atcgtgcgta aactgaatga cgatcgtcag tacaccgccg
     7501 ccgatggctc tgaaatttct ctgcacggac gctcgctgct gtttatccgc aacgtgggtc
     7561 atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc
     7621 ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc
     7681 gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg
     7741 ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa
     7801 tgggcattat ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt atcgctcagg
     7861 cacgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc
     7921 attcggtgat ggaagctggc ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga
     7981 tcaaagccta cgagcgtaat aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag
     8041 cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga
     8101 agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcgcca actgccgcta
     8161 cgctccatgc gatgcactac caccaaacca acgtacagag cgtgcaagcc aacattgccc
     8221 agactgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact attccggttg
     8281 ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg
     8341 ggattctggg gtacgtggtg cgctgggtgg agcaggggat tggttgttca aaagtgccgg
     8401 atattcacaa tgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata
     8461 tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga
     8521 atatggcgaa agtggttgat cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg
     8581 ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga
     8641 aacagccaaa cggctatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa
     8701 gtcattaata gcgtcaaaaa aagccccggc aagttttatt gtcggggcta tgatagtaac
     8761 tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa
     8821 atacccgtag cgcattacta gcgggcctat aacaacaata tggacatctt aacgatactc
     8881 tgcgcctgcg tgtgcagaac agaaaccctt acccgatata cttgttatgc cagaggtagc
     8941 aattgcggca accgatctga ctggcatgga tgcagatgca atggtggaag atataaatta
     9001 cacgataatt acagatgttc agattaccga acgaactaaa acctatgtca gagcagataa
     9061 tgctaccact tttcggcagg gaacatcagg caccgaaatt caggctagca cagaaaccag
     9121 caaccaggtt aatctgaaat ttgaaggggc taaacctctt ctggaaatac aactgaaata
     9181 aatatcctcc ggctatgccg gaggatattt attttaattt gaagcctgat ttacacgcag
     9241 ctctgcgtag ggcagggtat catagtcagt taacgcattt ttcttatagg ggtttcctat
     9301 ccagcgagtg atttctttga aatgctggtt taatatggaa cgctctggct catagttatc
     9361 ataacgtaaa ccagccaaat ccgaccaggt atggataaaa tcagccagac tgtatttacg
     9421 gttaatgtac tcagaaaatg tatgatgtcg attaagtagc catttttcag aggcccagag
     9481 aataaatgga atattgtaca taggtcgggt tggggtgtct tcgttacgtc ccagcatttt
     9541 atgtggtggc gtatcgaata cttcctctcc atggtcagaa aaatagaaaa gaaaaccatc
     9601 aggggctgtt gctttgaaag ttttgatcaa cgttgcaaca acatggtcat taaatatatt
     9661 agcattatcg taatcgttat ataattccag ttcattatca gtaagtcctt ctggtgcatg
     9721 ttccctgtcc ccatcgaata ctccctgacc ttcaggataa cggtatttat atttgatgtg
     9781 tgttcccaac aagtgaataa taacaaattt tttgggagct ggatctgcca gtacttcact
     9841 aaacggagcc agtacgttag tatcgtactc acgggcactt tgcgttctct gctgattcat
     9901 gtagtactgt ttatctgttt gccgagagaa gactgtcagc attgtgttgc gtgcagtcat
     9961 cgtttgttga ttggtgatcc agaatgtttt gtaaccggcc tgtttcatta tattcatcag
    10021 ggaaggttgt gtcaggtaga gttcgggctg ctgttcatca gcgaaagtca atacctgctg
    10081 tagtacttcg atggtatatg gtcttgagga gacaacatta ttaaacacaa acatattttg
    10141 ttccgttttg tataattcat ctagtccggg ggttgttttt ctgggatatc catacagact
    10201 cattctccca cgctgggttg attctcctat caccagaacc agtgttcgtg gggtatctcc
    10261 ggaaatatcg ttaaagtttt tcagagcggg gagcgcatta ttttcctcca gcaaacggtt
    10321 cagagaatta agctggagac ggtactgata atatccggta ataaattgcc agggggcagc
    10381 gggctccata cgggatgcca ggcgtcccag agtattgtca atagtcctgt ttttaacaac
    10441 agtatttatt aatacagggt gcagaattaa accgtagata ataaataaag gtataatgta
    10501 gcgccagtat gatggtatat atatcgggct aagacgagtc catagcagaa tagcaatagc
    10561 tgtatatatt gtcgatataa atagtatttt taaactaaaa tactgactaa aatattcgct
    10621 ggcttcactt gtattacttt caaacatgac gaacagtaca ctctgagaga attcctgccc
    10681 gtagattaga taatagcaaa gggcagcaag agatgtgccc cataaaacca cgccgattaa
    10741 tgctgctgta acctttattc tctctgggaa aagaaaaaca gggattaacc agagagagct
    10801 gaaaagtagc gagtcacgaa gtccattggt gccactatat ccagtcagac aaatgataat
    10861 ctgaagtaaa gtggaaaaat accagaaata aatgagagcc catcccaggg ctttccagct
    10921 aaaaacatgt tcatgttttg ttaatgtctt ctgcatatta ataaacctgc acgggaattt
    10981 actattgtaa tttttatatg agatgtatta aaacatttca tacagatttt ttgataagca
    11041 catagtttca atattcacta taatcgtttt ctgctgtgtt gtgtttgatc ctttttcaaa
    11101 gaggcgctaa tcttccactt gccaaggtta gatggtcagg gtccgggtta aacaaaatgt
    11161 ttgacaggga gcctttccat atgctgtctt tccagcgtgg cggtccgtaa tgtactaatt
    11221 ttaagagagg tccgcaatct tccagtggat ggttcatctg cctctctatt accgttacca
    11281 tcgtcttgcg tcctgtgcta tcgcctgtaa cgacatcagc gtcactggta ttaacaaact
    11341 tatagttgaa gccagaactg aaccgccccg gaaatcctgg agactaaact ccctgagaaa
    11401 gaggtaaaca ggatgactaa aaatactcgt ttttcccccg aagtccgtca gcgggcgatt
    11461 cgtatggttc tggaaagtca ggatgaatat gactcacagt gggcggcaat ttgttccatt
    11521 gccccaaaga ttggctgtac gccggagact ctgcgtgtct gggttcgcca gcatgagcgg
    11581 gataccgggg gcggtgatgg tgggctcacc agcgctgaac gtcagcgtct gaaagagctg
    11641 gaacgtgaaa atcgtgaact gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat
    11701 tttgcgaagg cggagttcga ccgcctctgg aaaaaatgat gccactgctg gataagctgc
    11761 gtgagcagta cggggtcgga ccggtatgca gcgaactgca tattgccccg tcaacgtatt
    11821 accattgtca gcaacagcga catcatccgg ataaacgcag tgcccgtgcg cagcacgatg
    11881 actggctgaa gagagagata cagcgcgtat acgatgaaaa tcatcaggtg tacggtgtgc
    11941 gtaaagtctg gcgtcagttg ttacgggaag gaatcagggt ggccagatgt acagtggcgc
    12001 gcctcatggc ggttatggga cttgccggtg ttctccgggg taaaaaggtc cgcactaccg
    12061 tcagccggaa aaccgttgcc acaggtgacc gcgtaaaccg tcagttcgtg gcagaacgtc
    12121 ctgaccagct gtgggtggct gattttactt acgtcagcac atggcagggc ttcgtctatg
    12181 tggcgtttat cattgatgtg tttgccggat acatcgtggg ggggcgggtc tcatcgtcta
    12241 tggaaacgac attcgtgctg gatgcgctgg agcaggcgtt gtgggcccgt cgtccgtctg
    12301 gcaccatcca ttacagcgat aaaggctctc agtatgtgtc actggcctat acggagcgac
    12361 taaaagaagc cggattactg gcatcaacag ggagtacagg cgactcgtat gacaacgcga
    12421 tggctgagag catcaatggt ctttacaaag cggaggtaat acaccgtaag agctggaaaa
    12481 accgtgcaga agtggaactg gccacactaa cgtgggtgga ctggtataac aatcgacgat
    12541 tgctgggaag gctgggccat actcctccgg cagaagcaga aaaagcttat tatgcttcca
    12601 tcggaaacaa tgatctggca gcctgagttc acagataaaa cactctccag gaaacccggg
    12661 gcggttcaaa gagtcacttc gtctcttggc taacgcagcg tgatgagctg gagtgccagg
    12721 cgtgggagaa caacgacagc agcattcgta ccggggacga tgacggtttg ctgtgtcagt
    12781 gcctggggga tatctgcagc aaggcgtttt cccagacggg gcgcgagttt gcagagctgg
    12841 gttgccagtt ttttctcacc cagtaaagcg agtttttcgg gtgagggata tcgctggaga
    12901 agatcaagta ctgcaggatg atccagtcgc gggccgagaa cgcgctccag agccggatgt
    12961 atctgggtca acaagccgcg gatgcggttg ctggcctgtg ttgtctgtgc ggcaagatca
    13021 tcatcgaagc cgcaaagcat ggagagttcg gcgatttgtt cgtcggccag ttttagagag
    13081 cgaagagcat gaggcagagt acgtgcagct tcggcaatga tggccgcatc acgggcatcg
    13141 gttttggctt caccggagtg caaatcggcg atgcgtcgca ttgccagtcc aggaaggtat
    13201 ccgacaagga cgccttcaga gcgggcaacg gcgacgggta acgccctgat ggtagcaggc
    13261 tggtcaacga ccagcagtat ctgtccatgc tgtttcaggt cagatatcag cgctctgagt
    13321 ttgttttcgt cgttgggtaa tactctgtcg aacaaacgtt tacctgaacg attaacggca
    13381 acggcgtaat gggtgtcttt acctacatca acgccaataa agaccaggat tgattcgtaa
    13441 tcgctggatt cgggcattgt gtctccccgt ttatacgggt taaccagata accttgggga
    13501 gcaggtaccg gcatccacgt tacagactgt cccgacatga tgtctgacca ggcccctatc
    13561 agcggttacc agcgccccac cagacccggt gacatccccc cgaatcatgg acgactgggg
    13621 gaattaatca tgccggggct ggctaacacc ccattacaag ggtacgaata aagtaacggg
    13681 gagttacagg cactgaaact atcatcagag atactgttgc caaatggcgt aaaggttgga
    13741 tcccgccgtt tgctattgcc gcccgacttc cttcactgtc cctgggaagc tgcggggtgg
    13801 tgccctggaa aataatcagg gggaagaaaa ttatgcgtcc cgatttgtga aacagaaccg
    13861 gaacagaaaa tagcgcagca actggcactc gagttctacc gtattctgcc ccccaaataa
    13921 atcacagcta aacagttggt tcactcaagt ccacgaaagc ggttcactag aatttcagcg
    13981 cgtagccacg gggatggagc cgatactgca gctatatgtg agacaattgt cagcgccagt
    14041 gatataagac ggtaatggag atgcggacgc ggcccttaat tcagagctag ccgagtataa
    14101 atttctggac aagtatgtaa atgatgagtt aaaaaataat ccgtgcattt ccggtgatga
    14161 tattagttgt tggtaacgaa atctcattag cggaccggaa acgcacttaa cctaaaacat
    14221 tgccaccgtt ccgccaggag ttgcagtggc aacgtatgct accgtcagtg atagcgtttt
    14281 gttgttatca gaactgatag ttcagcgaca tccagtaatt tcgctcaggt atcacatatc
    14341 ccgttgttga tgatcccgtc tggaagtaat caccggcata aagcgtactc ttaccggcac
    14401 tgtacagact cacgtcactg taatccttgt tgagcaggtt attcactgcc gcattcagcg
    14461 tcagggcatc cgtcaccttc cacgacagac ctgcatccac caccgtccag gctttcaggt
    14521 attctccttt ctcatcatat actttcttct gtacagcgct cagcgacgaa taattctggg
    14581 tgaaacgtgg tgttttcccg cgataacggg cacccagcca tgatgccacc tcttcggtga
    14641 tctgccagtt cagtttcgca ttcaccatgt gttctggggt ataactcagc ggtgcacctt
    14701 tattatcacc atcacgttgt tcacttcggg tccaggtgta attcagcgac agcgtgacat
    14761 cctctgacca tagcggcaat gtgccggcaa attccacacc gtgcaaccgg gcctttccgc
    14821 tgtttatata gctattggtg ttatcattta tggaataaga gacaatcttg ttagagaagt
    14881 cagtcataaa acctgtgata ttggcgttca gaccggcggg gttatcgtaa tacaccccag
    14941 cctcataact gacgctctct tccggcttca ggtcggggtt accaagtaga tttgtttttc
    15001 cctgcccgga cacaccacta atccctttat ggagctgccc cattctgggt gccttatatc
    15061 ccgtggtcac accgcctttc agcgtccagg catctgccac atcccagacc agatatgcac
    15121 gcggactgaa gtgccccccg aattgctcat gatgttcata gcggctgccc gcagtcagcg
    15181 caagtgcatc cgtgagatgc cactcgtcct cagcaaatac cgaccagctt ttctgccgga
    15241 aagtttcacc tgtgctggca aggacaaccc catctttcat ggacgaactc tgatactcgc
    15301 cccccacagt aaccagatga gattctccca gaggggtaag cagcaatgaa ttcaggataa
    15361 ggttagattc cttaagctcc cgcagttgac cggcaaggcc ccatttgtcg cgtttcagta
    15421 cattgcgtac aagctcacga cctttatttt ccgtctcgtt ccagttcaga tacgatttcc
    15481 atgttccgaa ggtgaaagta tgatcatagc cagctgaaat tttgtttcgc tcatagcgca
    15541 gggtccggtc atatcccccc gtcagactcc ccagttgccc atcccggtta tcataacgct
    15601 gccgggtggt atccatatca aaccagagca catcctgctc cgacgccttc cagtcaagac
    15661 gtgcaccaag attataattc tgtgactccg tgggataagg aatacgcgtg gctgctgtat
    15721 cgctcagtga tgtgaccgat gagccctgac gctgttgtgt gctaccgcgt acctgcaggc
    15781 tgacagaatc atccacaagg ggaccactgc tccagaaatt aaactggctg ctgttacccc
    15841 atttgttgct ttcctgcaga ttcagccctg cattgacgga agagagccat ttgtctgcat
    15901 tctttctggt aatgatattc accacaccgc ccatcgcatc agagccatac agtgtggaca
    15961 tcggccccct gataacctca atacgctcaa tggcggccag agggggcatg aacccggtat
    16021 tcatggcaga aaaaccgttg ggagtcacgt cactgcttcc gccctgacga acaccatcaa
    16081 tcagtatcag cgtgtaactg gctggcattc c
//
LOCUS       DAZFDP010000111        16039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFDP010000111 DAZFDP010000000
VERSION     DAZFDP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(385..1647)
                     /locus_tag="ACHJ1G_003552"
     CDS             complement(385..1647)
                     /locus_tag="ACHJ1G_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4765339.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1811..1895)
                     /locus_tag="ACHJ1G_003553"
     tRNA            complement(1811..1895)
                     /locus_tag="ACHJ1G_003553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:Leu,seq:caa)
     gene            2091..3110
                     /gene="ahr"
                     /locus_tag="ACHJ1G_003554"
     CDS             2091..3110
                     /gene="ahr"
                     /locus_tag="ACHJ1G_003554"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4765340.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3240..4742
                     /gene="yjgR"
                     /locus_tag="ACHJ1G_003555"
     CDS             3240..4742
                     /gene="yjgR"
                     /locus_tag="ACHJ1G_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4765341.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4861..5943)
                     /gene="lptG"
                     /locus_tag="ACHJ1G_003556"
     CDS             complement(4861..5943)
                     /gene="lptG"
                     /locus_tag="ACHJ1G_003556"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4765342.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5943..7043)
                     /gene="lptF"
                     /locus_tag="ACHJ1G_003557"
     CDS             complement(5943..7043)
                     /gene="lptF"
                     /locus_tag="ACHJ1G_003557"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4765343.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7106..7156)
                     /gene="ytgA"
                     /locus_tag="ACHJ1G_003558"
     CDS             complement(7106..7156)
                     /gene="ytgA"
                     /locus_tag="ACHJ1G_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4765344.1"
                     /translation="MSIIILATISTTQENK"
     gene            7310..8821
                     /gene="pepA"
                     /locus_tag="ACHJ1G_003559"
     CDS             7310..8821
                     /gene="pepA"
                     /locus_tag="ACHJ1G_003559"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4765345.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9175..9618
                     /gene="holC"
                     /locus_tag="ACHJ1G_003560"
     CDS             9175..9618
                     /gene="holC"
                     /locus_tag="ACHJ1G_003560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4765346.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9618..12473
                     /gene="valS"
                     /locus_tag="ACHJ1G_003561"
     CDS             9618..12473
                     /gene="valS"
                     /locus_tag="ACHJ1G_003561"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4765347.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12527..13723)
                     /gene="yjgN"
                     /locus_tag="ACHJ1G_003562"
                     /pseudo
     CDS             complement(12527..13723)
                     /gene="yjgN"
                     /locus_tag="ACHJ1G_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13916..14419
                     /gene="yjgM"
                     /locus_tag="ACHJ1G_003563"
     CDS             13916..14419
                     /gene="yjgM"
                     /locus_tag="ACHJ1G_003563"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4765348.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14465..14881)
                     /gene="rraB"
                     /locus_tag="ACHJ1G_003564"
     CDS             complement(14465..14881)
                     /gene="rraB"
                     /locus_tag="ACHJ1G_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4765349.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15043..>16039
                     /gene="argF"
                     /locus_tag="ACHJ1G_003565"
     CDS             15043..>16039
                     /gene="argF"
                     /locus_tag="ACHJ1G_003565"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4765350.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATL"
BASE COUNT         4139 a         4040 c         4134 g         3726 t
ORIGIN      
        1 atcctagaaa taaaatacca attgcttaat ttattaggaa ttaatggagt tacagattta
       61 agtcaaaagg aaaaggaaaa gttgaaaaaa agttttatag aaagaattac gatccagact
      121 agtaatgtat ctaaaaaatg ataaaaccac accgactaaa actgtaatga acagagttag
      181 tcggctggtt tctataacaa aagccatacc gtttgaacac tttttgtttt ttcactatca
      241 tctcagaatg gccattgcac ctcgctctaa aataatgccc cttggttgag gttttcccgc
      301 attttaaaag agcatttcaa tttattatta tataattctc tcacacaatt accaaattag
      361 ttaatgtata atatttcagt tacattattt ccccatgaat ttagcaaatt cataaggtgt
      421 aacagagtta tttcgattgg cctcaagaaa atcagcccac cattgaacca ttaatctacg
      481 ttcgtctaaa tgctcagagg tatgaatata tgctgcacgc acattgttac gctctgtatg
      541 actcaactgc cgttctatcg cgtcatcgct ccataagcct gattcaccaa gcgcgccacg
      601 cgccatcgtc ctaaatccat gcccacagac ctcagttttc gtgtcatacc ccatcgcacg
      661 caaggcgcta tttactgtgt tctcactcat aactttggcc gcgtcgtgat cccctggaaa
      721 aagtaactct ttatcgccac taatctgctt gagttgctct aacaaaacca tcgcctgtcg
      781 actaagagga acgatatgct cctctttcat cttcatgcca cgatacgagt aacgcacgcc
      841 tttaatttct tctcgctttg caggtatacg ccaaagagat ttatcgaagt cgaactcatc
      901 ccatcgcgcg aaacgtaact cactggaacg tacaaaagtt agtaaggaaa gctcgaccgc
      961 gatccttgtc attacacgac cacgatatgc agcaagacgt gcaagaaact cagagaagcg
     1021 gctagagggt aaagctggat agtgtcgtgc tttggttgta gataacgcac cggccatgtc
     1081 actggctgga ttagagtcga tgtaatcgtt ctgtacggca taacgcataa tggccgtgac
     1141 gcgctgttgc aggcgctgag cgacatcgtg tttgccactg gcatcaactt ttttaatcgg
     1201 ggctaacagg tggctggttt taagctgccg aatgtcggac gaaccgatat gaggaaaaat
     1261 ataaagctca agatagcgca gaatgcgtga tcgatggtct tcactccagc gcttattact
     1321 ggcatgccat tcacgagcaa ttgtttcgaa agtgtatgcg cccgaattct cagcctgagc
     1381 ttctttctgc tcggcttttg ggtcaatgcc ctgcactaag agctttttag cctcgtcacg
     1441 ctttgctctt gcctgagcaa gtgtcaccat gggccaaaca ccaaaagcga ggcgatcctc
     1501 ttttttgtca gaggggcgtc tgtatttcat gcgccagtat ttagaaccct tagccgaaac
     1561 ctcgagatac aaaccgccgc catcggccat tttgtaggtt ttctcttttg gctttgctgt
     1621 ctcgacctgt ctggcgttga gcttcatttg ggggcacatt tctaatcgaa gttaagatgc
     1681 ccccaattat gcccccaata gcatccggat ttcaacggac tagctcggac agctcaggac
     1741 agaaaaaccg ctacaggcat tgattttaaa gggtttgctg gattttctcg gatggtcttg
     1801 gaagtactaa tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt
     1861 gaatcaactg cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt
     1921 gtggattata cctgtcgcac gccaccatgc aagcgccagc ttgcacccac ctcgctaagt
     1981 gctgaaattt tcagcattac actcagcgat atgtaacctt tgtcacactc caggcacccc
     2041 gccctgccat gctctacact tcccaaacaa caccagagaa ggacccaaaa atgtcgatga
     2101 taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag tacgatcccg
     2161 gtgagctgaa gccacaagat gttgaagtgc aggtggatta ctgcgggatt tgtcattccg
     2221 atctgtcgat gatcgataac gaatggggat tttcacaata tccgctggtt gccgggcatg
     2281 aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt ttgcaggtcg
     2341 gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac acctgtatta
     2401 gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat cgcggtggct
     2461 ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa aatattgata
     2521 tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc
     2581 accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg
     2641 ctataaaact tctgcacgca atgggatgcg aggtgacggc ctttagttct aatccggcga
     2701 aagagcagga agtactggcg atgggtaccg ataaagtggt gaatagccgc gatccgcagg
     2761 cactgaaagc cctgtcgggg cagtttgatc tcattatcaa tactgtgaac gtcagcctcg
     2821 actggcagcc ttattttgag gcgctgacct atggcggtaa tttccatacg gtcggtgcgg
     2881 ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgaccgc agcatctctg
     2941 gttctgctac cggcacgcct tatgagctgc gtaagctgat gcgttttgcc gcccgcagca
     3001 aggttgcgcc gaccaccgaa ctgttcccga tgtcgaaaat taacgacgcc atccagcatg
     3061 tacgcgatgg taaagcgcgc taccgcgtgg tcctgaaagc cgacttctga tccctttgcc
     3121 gggagcctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
     3181 agcggtgaag agtgcacagc cgttgcctat actccaggca ggaaactgga ggaaatctca
     3241 tgagcgaacc cctgttaatt gcccgcacgc cggacacaga actgttttta ctgccgggaa
     3301 tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaact gtcacgctgc
     3361 aaaaactggc ggagtctcta tcggaaatcg gcgtgccggt gtttatggcc gatgtgaaag
     3421 gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc gcaaggctta
     3481 aaaatatcgg cgtaaatgac tggcaaccgc atgctaatcc ggtggtggtg tgggatatct
     3541 ttggcgagaa aggccatccg gtgcgggcga cggtttcgga tctggggccg ctgttgctgg
     3601 cgcggctgtt gaatctcaac gatgtgcaat ctggcgtgct gaatatcatc ttccgaattg
     3661 ctgacgatca ggggctgtta ctgctcgact ttaaagatct gcgggcgatt acccagtaca
     3721 tcggcgataa cgccaaatct ttccagaatc agtacggaaa tatcagtagc gcatcggttg
     3781 gtgccatcca gcgcgggctg ttgtcgctgg aacagcaagg cgcagcacac ttctttggtg
     3841 agccgatgct ggatatcaaa gacttgatgc gcaccgatgc caacggtaaa ggcgttatta
     3901 atatcctcag cgccgagaag ctttatcaga tgccgaaact gtacgccgcc agcctgctgt
     3961 ggatgatttc agagttgtat gaacaattgc cggaagcggg cgatctggag aagccaaaac
     4021 tggtgttttt cttcgacgaa gcacatctgc tgtttaacga tgcaccgcag gtactgctgg
     4081 ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
     4141 cgcaaaaccc gtctgatatt ccggataatg tgctcggcca gctcggtaat cgcgttcagc
     4201 acgctttgcg ggcttttacg ccaaaagatc agaaagcggt aaaagctgcg gcgcaaacca
     4261 tgcgggccaa tccggcgttt gataccgaaa aggcaatcca ggaactgggg accggcgagg
     4321 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
     4381 ttgcgccttg ttcgcggatg ggaccggtga cggaagatga gcgcaatggc ttgattaacc
     4441 actccccggt gtatggcaag tacgaggatg acgtggaccg ggaatccgcc tatgagatgt
     4501 tgcaaaaagg cgtacaggcg agtattgaac agcaaaataa tcctccagcg aaagggaaag
     4561 agatagcggt agatgacggc attcttggtg gattgaagga tattttgttc ggcactaccg
     4621 gaccacgcgg cgggaagaaa gatggtgtgg tgcagacaat ggccaaaagc gccgctcgcc
     4681 aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctgggaggg agaagaaggt
     4741 aagtcaggcg cgatctgtcc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
     4801 atcgaactcg tatttgactt tatcaacaat gtgaaaggga gctttcgctc ccttgcttgg
     4861 ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcgc ttggcaacag
     4921 tgcgccgata atcggcggga tgccgtaaac caacgtcagc gggccgaaga tctggtccag
     4981 tacgtagaag acaaaaccga agctgatacc ggtgaccaca cgcacgccca tcggtacgct
     5041 acgcagtggg ccaaagatga acgacagcgc catcagcatc atcaccgcca cggatagcgg
     5101 ctggaagatt ttgctccaca tattgagctg ataacgtccg gcatcctgac cgctcgactt
     5161 cagatacttc acgtagttgt gcaaaccgct gattgagagt gcatccgggt ccagcgccac
     5221 cacgcccagt ttgtctggcg tgaggttggt tttccaggtg ccgctcaccg tctgcgaacc
     5281 ggtgatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac gccagacttt
     5341 atgttccggg tcaaacttcg cagtagcggc atagcgtacg gattgcagac gacgattttc
     5401 gttaaaggca taaatgctga tgccacctaa ctcttcgtca cctttaaccc gctcaatgta
     5461 gacgaagttg ttgccatctt tcgcccataa gccttgctgg gtagagagca acgagccgcc
     5521 gtacatcgcc tgcgcacggt agttacgcgc catctgctcg ccctgcggcg cgacccattc
     5581 gccaatcgcc atcgtcagca agaccagcgg aatggcggtt ttcatcaccg acagcgccac
     5641 ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat
     5701 cccaagacca agcaacgccc caagcagagc cgccatcggg aagaagattt gcacatcttt
     5761 cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc
     5821 ttttttcaac tgatcgacaa atttgataat gcccgacagc gacaccagca tgaacagcgt
     5881 catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg
     5941 catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc
     6001 caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagc
     6061 gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa
     6121 agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg tgggttaacc
     6181 acgctcagcg gtacgaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc
     6241 cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgca catgtccatc
     6301 tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc
     6361 tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg
     6421 ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc
     6481 accacagaag gacgtgcatt accttttggt cgaatttgtg cgaggaacac atctttgaaa
     6541 tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc ttgctggaat
     6601 tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc
     6661 tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg
     6721 aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaggcc gcaggcatgc
     6781 attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac
     6841 aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc
     6901 accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga
     6961 cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc
     7021 accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat
     7081 tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta
     7141 agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa
     7201 taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa
     7261 ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag
     7321 tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga
     7381 accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag
     7441 cgccctgcta cgtcggggcg aactggaagg aaaaccgggg cagacattgt tgctgcacca
     7501 tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct
     7561 ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg
     7621 ctcaatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg
     7681 gaaagtgcgt caggctgtcg agacggcaaa agaaacgctc tacagtttcg atcagctgaa
     7741 aacgaacaag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg
     7801 ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat
     7861 taaagcagca aaggatcttg gcaatatgcc gccgaatatc tgtaacgccg cttacctcgc
     7921 ttcacaagcg cgccagctgg ctgacagcta cagcaagaat gttatcaccc gcgttatcgg
     7981 cgaacagcag atgaaagagc tggggatgca ttcctatctg gcggtcggtc agggttcgca
     8041 gaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag atgcacgccc
     8101 aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcga tcaagccttc
     8161 agaaggcatg gatgagatga agtacgatat gtgcggtgcg gcagcggttt acggtgtgat
     8221 gcgtatggtc gcggagctac aactgccgat taacgttatc ggcgtgttgg caggctgcga
     8281 aaacatgcct ggcggacgag cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca
     8341 aaccgttgaa gtgctgaata ctgacgctga aggccgcctg gtactgtgcg acgtgttaac
     8401 ttacgttgag cgttttgagc cggaagcggt gattgacgtg gcgacgctga ccggtgcctg
     8461 cgtgatcgcg ctgggtcatc acattaccgg tctgatggcg aaccataatc cgctggccca
     8521 tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga
     8581 cgagtatcag gaacagctgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc
     8641 tggtggggcg attaccgcag gttgcttcct gtcgcgcttt acccgtaagt acaactgggc
     8701 gcacctggat atcgccggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg
     8761 tccggtagcg ttgctggcac agttcctgtt gaaccgcgct gggtttaacg gcgaagagta
     8821 attgcgtcag gcaaggctgt tattgccgga tgcggcgtga acgccttatc cgacctacac
     8881 agcactgaac tcgtaggcct gataagacgc gcaagcgtcg catcaggcaa tgctgttatt
     8941 gtcggatgcg gcatgaacgc cttatccgac ctacacagca ccgaactcgt aggcctgata
     9001 agacacaaca gcgtcgcatc aggcaatgct gttattgtcg gatgcggcgt gagcgcctta
     9061 tccgacctac acacagcact gaactcgtag gcctgataag acacaacagc gtcgcatcag
     9121 gcgctgcggt gtatacctga tgcgtattta aatccaccac aagaagcccc atttatgaaa
     9181 aacgcgacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     9241 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt gctcatcgcc
     9301 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     9361 agctttgttc cgcataattt agcgggagaa ggaccgcgtg gcggtgcgcc ggtggagatc
     9421 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacatgc
     9481 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     9541 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     9601 acggcaacct ggaaataatg gaaaagacat ataacccaca agatatcgaa cagccgcttt
     9661 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9721 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9781 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9841 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9901 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9961 gggaatggaa agcggaatcc ggcggcacga ttacccgtca gatgcgccgt ctcggcaact
    10021 ccgtcgactg ggagcgtgaa cgcttcacga tggacgaagg cctgtccaat gcggtgaaag
    10081 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaaacgc ctggtaaact
    10141 gggacccgaa actgcgcacc gctatctctg acctggaagt ggaaaaccgc gaatcgaaag
    10201 gttcgatgtg gcacatccgc tatccgctgg ctgacggtgc gaaaaccgca gacggtaaag
    10261 attacctggt ggttgcgact acccgtccgg aaaccctgct gggcgatacc ggtgtagccg
    10321 ttaacccgga agatccgcgt tacaaagacc tgattggcaa atatgtcatt ctaccgctgg
    10381 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
    10441 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
    10501 tgccgatgat caacatcctg acctttgacg gcgatatccg tgaaagcgcc caggtgttcg
    10561 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagaa ttccagaaac
    10621 tggagcgttt tgccgcacgt aaagcagtcg ttgctgcggt tgacgcactc ggcctgctgg
    10681 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtagttatcg
    10741 aaccaatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcggttg
    10801 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtatttct
    10861 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10921 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10981 gcaaggaaaa taacctcggt gccgacgttg ccctgcgtca ggacgaagac gttctcgaca
    11041 cctggttctc ttctgcgctg tggaccttct ctacccttgg ctggccggaa aacaccgacg
    11101 ccctgcgtca gttccaccca accagcgtga tggtatccgg cttcgacatc atcttcttct
    11161 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    11221 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggccagaaaa
    11281 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    11341 cagaactgct ggaaaaacgt accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    11401 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    11461 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgcc
    11521 tggaaggtta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    11581 acacagaagg tcaggattgc ggctttaacg gcggcgaaat gacactgtcg ctggcggacc
    11641 gctggattct ggcagagttc aaccagacca tcaaagcgta ccgcgaagcg ctggacagct
    11701 tccgcttcga tatcgctgca ggcattctgt atgagttcac ctggaaccag ttctgtgact
    11761 ggtatctgga gctgaccaag ccggtaatga acggtggcac cgaagccgaa ctgcgcggta
    11821 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11881 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11941 ccatcatgct gcagccgttc ccgcagtacg atgcatctca ggttgatgaa gccgcactgg
    12001 ccgacaccga atggctgaaa caggcgatcg ttgcggtacg taacatccgt gcagaaatga
    12061 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    12121 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaaccctggc gcgtctggaa agcatcaccg
    12181 tgctgcctgc cgatgacaaa ggtccggttt ccgttacgaa gatcatcgac ggcgcagagc
    12241 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgt ctggcgaaag
    12301 aagtggcgaa gatcgaaggc gaaatcagcc gtatcgagaa caagctggcg aatgaaggct
    12361 ttgtcgcccg cgcaccggaa gcggtcatcg cgaaagagcg tgagaagctg gaaggctatg
    12421 cggaagcgaa agcgaagctg attgaacagc aggctgttat cgccgcgctg taatcaacac
    12481 cgtgatgaaa acgaaggccg gagaatgctc cggccttttt tatctcttac agaaatggta
    12541 aagaaggcat aattccccgt gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    12601 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12661 caatcatcca tattttcagc agtggataag ccagaccgcc cgtaatcccg gatatcacta
    12721 ccaacgcaca catgcgataa agcataccgt ggtaagttaa agttgagcga aaacgaatac
    12781 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12841 tggtgctaac agcaatccca aaataataga ttaactgcgc gattatcatt ttacgttgca
    12901 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacacagaa ctatcatacg
    12961 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta
    13021 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    13081 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    13141 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    13201 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    13261 tggccattaa aatcggcaag aaaaaggtta ctcaccagaa ccctttcata gagcaattaa
    13321 aactaaatct tacgccgttg agactagtca tcaaggcctg ataacgcagt ccttttgctg
    13381 ccataaatat aagcaaaacc aacagtgaca aagtcaaaac acagccaata agcggcatat
    13441 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    13501 aaacattccc accggtaatc ccataagaaa atcgttgtcc gttaacctcc atattagcat
    13561 agagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    13621 ttaacaacac attcaccaca caaataagaa aatatctctc acctgtatca tgaaaaacaa
    13681 acgaatggga cggaacatcc atttcattaa taacttgagc cataaaaatt ccctgaatta
    13741 aaaaatccat tgatataagt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13801 ctcttatcag taataggctg gcaaaaactt tattgcttta taagatgctt gcgcacgtaa
    13861 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13921 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13981 tgcccgcgtc attcgtcagg tatccgccga atacggtctt accgctgata aaggctacac
    14041 cgtcgccgat ccgaatcttg acgagctgta tcaagtgtat agtcagccgg gtcatgcata
    14101 ttgggtggtt gagtacgagg gtgaagtggt cggcggcggt gggattgcgc cattagcagg
    14161 tagtgaatcg gacatttgcg aactgcaaaa gatgtatttt cttccggcta ttcgcggtaa
    14221 agggctggcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    14281 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    14341 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    14401 gatgctacgc gaactctaaa aaaatgcccg ctagtgtgtg ctatcgggca ttttaatcaa
    14461 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccgtctt
    14521 cgccgttggg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    14581 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    14641 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14701 tcacttcgta accgagttta aacgcttcaa ctgctgcttt ttccagggtt tccagatcgt
    14761 ctgcggaaag atggtgttcg atggtataca gcgcgtccgg atcgctgcca tcttccagta
    14821 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14881 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14941 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataatca tccatataaa
    15001 ttgaatttta attcattgag gtgttagcca caggagggat ctatgtccgg gttttatcat
    15061 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    15121 gccgcgaagc tgaaagccga taagaaaagc ggcaaagaag aagccaaact cactggtaaa
    15181 aacatcgcgc tcatcttcga aaaagactcg acccgtaccc gatgctcttt cgaagttgcc
    15241 gcatatgacc agggtgctca cgttacttat ctcggcccaa gcggcagtca gattggtcat
    15301 aaagagtcga ttaaagacac tgcccgcgtg ctgggtcgca tgtatgacgg tattcagtat
    15361 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    15421 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    15481 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    15541 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    15601 gtcgcgccac aggcatgctg gccggaagct gcgctggtta cggaatgccg cgctctggca
    15661 cagcaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15721 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagagaa atgggctgaa
    15781 cgcattgcat tgctgcgtga ttatcaagtg aacagcaaga tgatgcagtt gaccggtaac
    15841 ccggaggtca aattcctcca ctgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15901 aagaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15961 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac tatcaaagcg
    16021 gtgatggtcg cgacgctca
//
LOCUS       DAZFDP010000112        15879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFDP010000112 DAZFDP010000000
VERSION     DAZFDP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..982)
                     /gene="ldtC"
                     /locus_tag="ACHJ1G_003566"
     CDS             complement(20..982)
                     /gene="ldtC"
                     /locus_tag="ACHJ1G_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4765351.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1126..4572)
                     /gene="mfd"
                     /locus_tag="ACHJ1G_003567"
     CDS             complement(1126..4572)
                     /gene="mfd"
                     /locus_tag="ACHJ1G_003567"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4765352.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(4700..5773)
                     /locus_tag="ACHJ1G_003568"
     CDS             complement(4700..5773)
                     /locus_tag="ACHJ1G_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4765353.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6035..7234
                     /gene="lolC"
                     /locus_tag="ACHJ1G_003569"
     CDS             6035..7234
                     /gene="lolC"
                     /locus_tag="ACHJ1G_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4765354.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7227..7928
                     /gene="lolD"
                     /locus_tag="ACHJ1G_003570"
     CDS             7227..7928
                     /gene="lolD"
                     /locus_tag="ACHJ1G_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4765355.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            7928..9172
                     /gene="lolE"
                     /locus_tag="ACHJ1G_003571"
     CDS             7928..9172
                     /gene="lolE"
                     /locus_tag="ACHJ1G_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4765356.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9201..10112
                     /gene="nagK"
                     /locus_tag="ACHJ1G_003572"
     CDS             9201..10112
                     /gene="nagK"
                     /locus_tag="ACHJ1G_003572"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4765357.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10128..10949
                     /gene="cobB"
                     /locus_tag="ACHJ1G_003573"
     CDS             10128..10949
                     /gene="cobB"
                     /locus_tag="ACHJ1G_003573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4765358.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11088..11876)
                     /locus_tag="ACHJ1G_003574"
     CDS             complement(11088..11876)
                     /locus_tag="ACHJ1G_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4765359.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(11873..12334)
                     /locus_tag="ACHJ1G_003575"
     CDS             complement(11873..12334)
                     /locus_tag="ACHJ1G_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO4765360.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            complement(12392..13438)
                     /gene="potD"
                     /locus_tag="ACHJ1G_003576"
     CDS             complement(12392..13438)
                     /gene="potD"
                     /locus_tag="ACHJ1G_003576"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4765361.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13435..14229)
                     /gene="potC"
                     /locus_tag="ACHJ1G_003577"
     CDS             complement(13435..14229)
                     /gene="potC"
                     /locus_tag="ACHJ1G_003577"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4765362.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14396..15514)
                     /locus_tag="ACHJ1G_003578"
     CDS             complement(14396..15514)
                     /locus_tag="ACHJ1G_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4765363.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(15483..15752)
                     /locus_tag="ACHJ1G_003579"
     CDS             complement(15483..15752)
                     /locus_tag="ACHJ1G_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4765364.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
BASE COUNT         3777 a         3993 c         4096 g         4013 t
ORIGIN      
        1 gatttttttt atccaggatt tacagagttt gtgggctcac ttgatgacgg cggacatcca
       61 ccggcatccc ggaacggaca tccatcacat gttgcatcac ttcagcgtca gtttgtgctg
      121 catctttaaa tgattgcatt gcgctattca gcgtaattgg cagcagctgc ggatcgtcat
      181 caatcttctc tgacagcggc tgatgtactt caaccagacg cgcaccgttt ggttcggcag
      241 agacttttat cggagtgttg atgatattca ctttggtgcc tggggtgacc tggctaaaga
      301 gtgttttgat atcgtcatcc cgcagacgaa tacagccaga acttacccgc atgccaatgc
      361 cgaaatcggc gttcgtaccg tgaagcaaat aaacgccgcc ataggccgcc agacgaatcg
      421 catgatggcc cattgggtta tccggtccag ccggcactac cgcaggcaac tcaattccct
      481 gtgctttata acgggcgcgg atgtttgccg ttggcgtcca ggttgggttt gcacgtttgt
      541 ctgaaacggt ggtcaccatt gtcggtgtca gcgtgtcacc acctaactga ccaataccta
      601 ttggatacac ggttaccgaa tttttacccg gcgggtagta atagagacgc agctccgcaa
      661 tattgatcac aatgccttcg cgcggcgcat ctggaagtag ggtttgcaac gggatcgtta
      721 acacgctacc cgcgcgcggt acgtaaggat caacgccagg gttagcctgt aacagagcga
      781 gaaagccgac gttatatttt ttggcgatgg cttccagaga accaccgtca ttttccacca
      841 catgaaattt gttttcgcca accagacgac tgcccgctgg cggcagcggc caggtgttgg
      901 cttttgccgg tagcgccagc gccacggcag cggcgaacgt aaaaaacgtt agccagcgag
      961 aaaaacgcgt tttgatcatc accaaaaatc cataataaat ataaggttat tgtaataaac
     1021 agataagcct gaattatgga tggtgacagt gtcggatagt gcagggaagt gcaaagaatt
     1081 tgtaaatgtt gcagatgggg gcgcagaaac gcccccgatt taccattaag cgatcgcgtt
     1141 ctcttccagt tcacgcataa actggcgtac ccattcgata cgcgttttcc gctcactcaa
     1201 atcctgaata aatttcaggc gcgtcggacc atcaaggcgg taatgctgcg gctgtttttg
     1261 cagcaaacca atcaaccagg ccggattaac gtgattcttc tcggcaaatt cgatcacccc
     1321 gcctttctca ttaccttcca gcttcctgat ccccagtttc tgcgcttgct ggcgcagacg
     1381 ggcaacatcc agcagggtac gcgccggatc cggcagcagg ccgaagcgat cgataagctc
     1441 gactttgatc tcttccagtt cgttttccgt tttggcgctg gcaatacgtt tatagaacga
     1501 caaacgcgta ttcacgtcag gaatgaaatc atctggcaat agcgacggca tccgcagctc
     1561 gacttctgtt tgctggctgg tgagatcttc cagcgacggc tcgcgtccgg ctttcagtgc
     1621 atcgacggcg ttttccagca actccatata cagcgagaaa ccgatggttt ccattgagcc
     1681 gctttgttct tcgccaagca gttcacccgc gccgcgaatc tccagatcgt gcgttgccag
     1741 cgcaaaacct gccccgagat cttccagcga ggcaatcgct tcaagacgtt tttgtgcatc
     1801 ggtagtcatc gcttttggat gcggcgtcag caaccatgca tatgcctgat gatgtgaacg
     1861 tccgacgcga ccgcgtaact ggtgcagctg cgccagaccg aagtgatccg cgcgttcaat
     1921 gataatggtg ttggctgtcg ggatgtcgat cccggtttcg ataatggttg tacaaaccag
     1981 cacgttgaaa cgttgatgat ggaaatcatt catcacccgt tccagttcgc gctcgcgcat
     2041 ctgcccgtga ccgatggcga tccgcgcttc tggcaccagt tctgccagcc gttcggcggc
     2101 tttctgaatg ttttccacat cattgtagag ataataaacc tgtcctccgc gcaaaatttc
     2161 acgcaggatc gcctcccgga ccaccaggct gtcatactca cggacaaagg ttttaactgc
     2221 cagacgacgg gcgggcggcg tggcgataat cgacaggtca cgcattccgc tcattgccat
     2281 attcagcgta cgtgggatcg gcgttgcagt aagcgtcaga atatccacgt tcgcgcgcat
     2341 cgctttaata cgctctttat gacgcacccc gaagcggtgt tcttcatcga caatcagcag
     2401 gcctaaatct ttaaacttga cgtcactttg cagcagtttg tgcgtaccga tcagaatatc
     2461 gattttccct tccgccactt ccgcaaggat ttgcgtctgc tctttggcgc tgcggaaacg
     2521 ggagatcatt tcgatacgta ccggccagtt ggcgaaacgg tcgcggaagt tgtcgtaatg
     2581 ctgctgcgcg agaagggtgg taggcaccaa caccgccacc tgcttgtggt tatctactgc
     2641 caggaaagcg gcgcgcatcg ccacttctgt tttaccaaag ccaacatcgc cgcacaccag
     2701 acgatccatt gccagcggct gacacatgtc gctaagtacc gcattaatgg cctgcgcctg
     2761 atccggcgtg gtttcaaacg ggaagctgtc gcagaacaac tgatactgct cacgatcgtg
     2821 tttaaacgcg aagccctctt tggcggcgcg ttgcgcgtag atatccagca attccgccgc
     2881 cacatcacgc actttttccg ccgctttctg ccgcgcgcgt gaccacgcat cgccgccaag
     2941 tttatgcagc ggggcgtttt cttccgcgcc acctgcgtaa cggctaatca gatgcagtga
     3001 cgacaccgga acatacagtt tggcgtcgtt ggcataggtg agcatcaaat actcgccagt
     3061 aatgccgccc gcttcgagcg tggtcattcc ggcatagcgc ccgacaccgt gctccagatg
     3121 gaccaccggc tgaccaatat gcagctccgc aaggttacgg atcagtgtat cggggttgat
     3181 ggtgcggcga gaatcctgac gacggcgggc aacacgttca ccgagcagat cgctttcgca
     3241 gatcagcgcc agattacgca ccttatcgac aaaaccatgt tcggcagcgc caatcatcag
     3301 ataacgccca cggtcgctgg cttcatcaag acgcataatg cgttgcggag caattttaat
     3361 tcgtgcgagc agttcaccca gcgcttcacg gcgaccttca ctttctaccg agaacaccac
     3421 cggaccgtcg aaagtctcga ggaacttacg cagcgcatcc agcggcgctt tttgttgtgc
     3481 ctgaacggcc aggtctggca gtttctggaa acctaaattg gcattcgcgg cttttgtcgg
     3541 taaatgttca gtttttagct gcacccgggg ccagtttttc agctctgaga agagctcgtc
     3601 cacccgcagc cagagcgatt gtggtggcaa cagcgggcgc atcggatcga cgccgcgatt
     3661 ctcaaaacgc gccagcgtgt cagcctggaa acgttcggca ctggtttcca gatcgccagt
     3721 attcacgagc aaggtattgg cagggaaata actgaacagc ggcggcagtg gttcgctgaa
     3781 gaacaatggc tgccagtact cgatcccggc aggtaatgtg cctttactca cttgctggta
     3841 aatatgttcc ggatcgcgct tcacttcgaa ggtatcgcgc cactggctgc ggaacagttc
     3901 aattgccgct ttatcggtcg gaaattcgtg cgcgggcagc agattgatcg cttctacttc
     3961 ctccagcgtg cgctggctgt cgacgtcaaa cacccgcagg ctgtcgattt catcatcaaa
     4021 gaaatcaaga cgataaggca gctcactccc catcgggaag agatccagca acgcgccgcg
     4081 cgtggcgtat tcgccgtgct ccatcacctg gtcaacatgg cgataaccgg cgctgtccag
     4141 ttgggttcgt aatgcatctc gtgacaggcg ctgacctttt ttcatcacca gcgcatgacc
     4201 gtggagaaaa ctgtgtgggc aaacgcgctg cataagcgta ttcaccggaa caatcagtac
     4261 gccacgctgc atcgtcggta gctggtaaag ggtggaaagg cgcgaggaga taatgtcctg
     4321 atgaggcgaa aaactgtcgt agggaagagt ttcccagtcc gccagattca tcaccatctg
     4381 atcggtgaac tggctgattt catcatgcaa acgcagagca ttttgcatat ctggtgcaat
     4441 gagtaccacc ggaccggcgt gacgttcggc aatttccgct accagcgttg cacaggctgc
     4501 gccggttaac tcgcccagca gacgctgctc acccgctttg acgggcagcg tataacgata
     4561 ttgttcaggc ataacggttg tcagattctc gttaggatat gcctcaacat atgggggcat
     4621 atctctgata agcaatgctg ttcattatcc gtaatcgaca tggtttagca aattgaatcg
     4681 ccccggctgg ggcgatggtt taacgagagt aactgacggc agcgggcagt gaaggcgggg
     4741 agaacaacag atcgctgaat gcacgctgtg aaagttttct taccagcaag cccgcaacag
     4801 tacagatgaa caaactgaca aagggataaa ccagcaggag tgtcagttcg acttgcggcg
     4861 accagcgtgc tgcgttcatt tgcgcaagca gagttaagct gaatatttca accagaatgc
     4921 gatgggtggt gtagatagca atggtgttgg agccaatcac attcagcagg ctggtggagc
     4981 gcataccgaa tcgttgctcg tattgataaa acagcttcat gatcaccaca atcgatacca
     5041 gcgacaacag cagcgagata gtaaacaacc aggcaccgac cgccagaacg gtaagcaaag
     5101 aagccatcag cagatggcgg cgcaacggca cctctttaac acaggtcatt attgttgcgc
     5161 caaaccatgc gccaaggctg taataaagca aattgcggat cacactgttc attccccacc
     5221 acggcgtggg aacgaaatta accgccacac tcagcagtac aaacaaggcg aatagtggca
     5281 gcgccagacg gctaaaaatt ttacatacca cgaaatagac aattaacgca tacagatacc
     5341 acaagctggt gctggcggtg atcatcccgt gcaggaactc accggtggaa tcggcataag
     5401 cggcattgga ggcattgctt aaatcgcgct caggtgccag ccactggttc agcgcactta
     5461 acgccagcca ctgcaccacg ccccaaagcg ccagcaccca gaagatgttc cagatccgtt
     5521 tatcgagaca atttccccac ggtacgctgt cgatatagcg gcgaatcaaa tagccggata
     5581 taaagaaaaa aaccggcata cgaaaggggg caaggtaaag attgaaatag atccagcatt
     5641 tgctcaggac ttccgataac ggatgctgaa aagtggtcag atgcggataa aaggtaatga
     5701 ccgagtgata aatcaccacc agacaaatac ataacccttt gatctggtta atccatagct
     5761 ctttttgttt catcaggaca acacgctctt ttttaattta catgcggttg atcctgcggg
     5821 ttatgacccg aggtgtaatg gggatctgtc tgtattggat gatttttcag attaagatca
     5881 ggcggcaaga ttgatgataa aacatggcaa tttagccgat tgatttacgg gggcttttca
     5941 gattagccct gacgatcact tacagttcag acgtttaccc atcttgcttt cgcttatata
     6001 ctcgtgtctt tgctacagca accagacgga tttcatgtac caacctgtcg ctctatttat
     6061 tggcctgcgt tacatgcgtg ggcgtgcagc ggatcgcttc ggtcgtttcg tctcctggct
     6121 ttctaccatc ggcattaccc tcggggtgat ggcgctggtc acagtattgt cagtgatgaa
     6181 cggctttgag cgcgagctgc aaaacaacat ccttggcctg atgccacagg caattctctc
     6241 ttctgagcat ggctctctta acccgcagca actcccggaa acggcagtca aactggacgg
     6301 cgttaatcgc gtcgcaccta ttactaccgg tgatgtggta ctgcaaagcg cgcgcagcgt
     6361 ggcggtcggg gtgatgctcg gtatcgaccc ggcgcaaaaa gatccactta caccgtatct
     6421 ggtcaatgtg aaacaaactg acctcgagcc ggggaaatat aatgtcatcc tcggcgaaca
     6481 acttgcctca cagctaggcg ttaatcgcgg tgatcaaatc cgcgtgatgg taccatctgc
     6541 cagccagttc acgccgatgg ggcgtattcc aagccagcgc ctgttcaatg tgattggcac
     6601 tttcgccgcc aacagtgaag tcgatggcta tgaaatgctg gtgaatattg aggatgcctc
     6661 gcgtctgatg cgttatccgg caggcaatat taccggctgg cgtttgtggc tggatgagcc
     6721 gctgaaagtt gactctttaa gtcagcaaaa actgcctgaa ggcagcaaat ggcaggactg
     6781 gcgtgaccgt aaaggcgagc tgttccaggc cgtacgcatg gaaaaaaata tgatgggctt
     6841 gctgctgagc ctgattgtcg ccgttgcggc gtttaacatt attacctcac tagggctgat
     6901 ggtaatggag aagcagggcg aagtcgcgat cctgcaaacg caaggcttaa ctccgcgaca
     6961 aatcatgatg gtctttatgg tgcaaggggc cagcgccggg attatcggtg cgatcctcgg
     7021 tgcggcgctt ggcgcactgc ttgccagcca gttaaataat ctgatgccga taatcggcgt
     7081 cctgcttgat ggcgcggcgc tgccggtcgc tatcgaacct ttacaggtca ttgttattgc
     7141 gctggtggcg atggctatcg cgctgctgtc tacgctttac ccttcatggc gcgctgccgc
     7201 cactcaaccc gctgaggctt tacgttatga ataagatcct gttgcaatgc gacaacctgt
     7261 gcaaacgcta tcaggaaggc agtgtgcaaa ccgatgtgct gcacaatgtc agtttcagcg
     7321 tcggcgaagg tgaaatgatg gcgatcgtcg gtagctctgg ttccggtaaa agcaccttgc
     7381 tgcacttgtt gggcgggctg gatacgccaa cctccggcga tgtgatcttt aacggtcaac
     7441 cgatgagcaa actgtcttcg gcggcgaaag cagagctgcg caaccagaag ctgggcttta
     7501 tttatcagtt tcaccacctg ctgccggatt ttaccgccct ggaaaacgtg gctatgccgc
     7561 tgctgattgg caagaaaaag cccgctgaaa tcaacagccg tgcacttgag atgttaaaag
     7621 cggtggggct ggatcatcgt gcgaatcacc gcccatctga actttctggc ggcgaacgcc
     7681 agcgtgtggc gattgcccgt gcgctggtga ataacccgcg cctggtactg gcggatgaac
     7741 ctaccggtaa cctcgatgcg cgtaacgccg acagcatctt ccagttgctt ggggaattga
     7801 atcgcttgca gggcaccgcc ttcctggtgg ttactcacga cctgcaactg gcgaaacgta
     7861 tgagccgcca gttagaaatg cgtgatggtc gtctgacggc ggaactgagc ctgatggggg
     7921 cggagtaatg gcgatgcctt tatcgttatt aattggcctg cgttttagtc gcggacggcg
     7981 gcgcggcggc atggtgtcgc tgatctccgt tatttctacc attggcattg ccctcggcgt
     8041 ggcggtattg atcgtcggct taagcgcgat gaacggcttt gaacgcgaac tgaacaaccg
     8101 tattctggcg gtggtgccgc atggtgaaat cgaggcggtg gatcagccgt ggactaactg
     8161 gcaggaagca ctggatcacg tgcaaaaagt gccgggtatt gccgctgccg cgccgtatat
     8221 caatttcacc gggctggtgg aaagtggcgc gaatcttcgc gcaatccagg tgaagggcgt
     8281 taacccgcaa caggaacagc gtctgagcgc attaccctcg tttgtccagg gcgatgcgtg
     8341 gcgcaatttt aaagcgggcg aacagcaaat tatcatcggc aaaggcgtgg cggatgcgct
     8401 gaaagtgaag cagggggatt gggtgtcgat tatgatcccc aactcgaatc ccgagcataa
     8461 actgatgcag ccaaaacgtg tgcgtttgca cgttgccggt attttgcagt tgagtggtca
     8521 actcgatcac agttttgcca tgatcccgct ggcggatgcc caacaatatc ttgatatggg
     8581 ttccagcgtg tcaggtattg cccttaaaat gacggatgtt ttcaacgcca ataagctggt
     8641 acgcgatgcg ggtgaagtga ccaacagcta tgtttatatt aaaagttgga ttggtactta
     8701 cggctatatg tatcgcgata tccagatgat ccgcgccatt atgtatctgg cgatggtact
     8761 ggtgattggc gtggcctgtt tcaacatcgt ctccacctta gtgatggcgg tgaaagacaa
     8821 gagtggcgat atcgcagtat taagaacgct gggggcgaaa gatggtttaa ttcgcgccat
     8881 ctttgtctgg tatggattgc tggcagggct gttcggcagc ctgtgtgggg tgattatcgg
     8941 tgtggtggtt tcactgcaac ttaccccgat tattgagtgg attgaaaagt tgatcggtca
     9001 tcagttcctc tccagcgata tctattttat tgatttcctg ccatcggaat tgcactggct
     9061 ggacgtcttc tacgtactgg tcacagcatt gttgctgagt cttttggcaa gttggtatcc
     9121 ggcgcggcgc gccagtaata ttgaccctgc gcgagtcctt agcggccagt aaaggcagta
     9181 cattaaaaca aggagcggca atgtattacg ggtttgatat tggtggaaca aaaattgcgc
     9241 ttggcgtgtt tgatagcggt cggcagttgc agtgggaaaa gcgggtgccg acaccgcgtg
     9301 acagctatga cgcattttta gatgcagtgt gtgagctggt agctgaagct gaccaacgtt
     9361 ttggctgtaa aggttctgtc ggcatcggta ttccgggaat gccggaaaca gaagatggta
     9421 cgctgtatgc cgccaatgtc cccgctgcca gcggtaaacc gctgcgtgcc gacctgagcg
     9481 cacgtcttga tcgcgatgta cgccttgata acgatgccaa ctgttttgcc ctttcagaag
     9541 cctgggatga tgaatttact caatatccac tggtgatggg gttgattctc ggcaccggcg
     9601 ttggcggcgg gctgattttc aacggcaaac cgattaccgg taaaagctac attaccggcg
     9661 agtttggcca tatgcgtctg ccggttgatg cgttaaccat gatggggttg gatttcccgt
     9721 tacgccgctg cggctgtggt cagcatggct gcattgaaaa ttatctgtct ggtcgcggtt
     9781 ttgcgtggct gtatcaacac tattatcatc aaccgttgca ggctcccgaa attattgcgc
     9841 tttatgatca aggcgatgag caggcaaggg cgcacgttga gcgttatctg gatttattag
     9901 cggtttgtct gggaaatatc ctgaccattg ttgaccctga cctggtcgtc attggtggtg
     9961 gcttatcgaa tttcccggca atcacaacgc aactggcgga caggctgcct cgtcatctct
    10021 tacctgtagc tcgtgttccg cgcattgaac gcgcgcgcca cggtgatgcg ggaggaatgc
    10081 gtggtgcggc cttcctacat ctaaccgatt aaacaacaga ggttgctatg ctgtcgcgtc
    10141 ggggtcatcg gttaagtcgt tttcgtaaaa ataaacgccg cctgcgcgag cgtttgcgtc
    10201 agcgtatttt tttcagagat aaagtggtgc cggaagcaat ggaaaaacca agagtactcg
    10261 tactgacagg ggcagggatt tctgcggaat caggtattcg tacctttcgc gccgcagatg
    10321 gcctgtggga agaacatcgg gttgaagatg tggcaacgcc ggaaggtttc gatcgcgatc
    10381 ctgaactggt gcaagcgttt tataacgccc gtcgtcgaca gctgcagcag ccagaaattc
    10441 agcctaacgc cgcgcatctt gcgctggcta aactgcaaga cgccctcggc gatcgctttt
    10501 tgctggtaac gcagaatata gacaacctgc atgaacgcgc aggtaatacc aatgtcattc
    10561 atatgcatgg ggaattgctg aaagttcgtt gttcgcaaag tggtcaggtt ctcgactgga
    10621 ccggagacgt tacgccagaa gataaatgcc attgttgcca gttcccggca cccttgcgcc
    10681 cgcacgtcgt ttggtttggc gaaatgccac tcggcatgga tgaaatttat atggcgttgt
    10741 cgatggctga tattttcatt gccattggta cttccgggca tgtttatccg gcggctgggt
    10801 ttgttcacga agcgaaactg catggcgcgc acaccgtgga actgaatctc gaaccaagtc
    10861 aggtcggtaa tgaatttgcc gagaaatatt acggcccggc aagccaggtg gtgcctgagt
    10921 ttgttgaaaa gttgctgaag ggattataaa gcaggaagca ttgcctgatg cgctacgctt
    10981 atcaggccta caaggggacg caattaattg aatttgcggg ttttgtgggc cggataaggc
    11041 gttcgcgccc catccggcat aatacacgcg cacttttccg gcctctttta tcgtttactc
    11101 gtcaataaca atgcggcaat aaacgtacag acaccaatta gccctgccca gagtggcgat
    11161 attctggtaa tcgcaaagct attatcgata tacaaacata aagcctgata acccatattg
    11221 gcaatagtca tcgccactgc aaattttaac gcataaataa aacgagtcgt gtagcaaaag
    11281 atccaggaaa ataagataaa cgtcgcagcc caggcgataa aacctaatgt gttggtgctg
    11341 ccggggggag cattttcatg ctgttgtcct ggatgcggat aatacgtcag tggcggcgtc
    11401 ggttgtttta atatctgtgc ttgtagaaca gccaatgcat tattgattcc ctgataccag
    11461 agatctgcat ttaaataagc cagcgtgtga ttttctatca gaaggccgag atgtttatcg
    11521 tccagaccac tttcaacctg gccgttggtg atcataatta accgtggttc gcgggtagta
    11581 aatagtaaca gaacacttcc ttttacatta ctcaatgaag atgacgcatg cgccagcatc
    11641 gctttaccat tttgtctgag actggcttca ttattaatgt cgggtaaaat cacaataaac
    11701 ggtgcaattc ccgtttgttg gcgaaaaagc tcggctttac gtgcgatctc ttcagcatcc
    11761 ggtacagtac cggtaacatc cgtaaaaagt gtcttcatta ttggaatcaa tggtttactg
    11821 gttgctacca gaggcaggag tatcagtaaa ctgagcagta taatgaattt tttcatgggc
    11881 aggacttccg tttcctgcga gattttttgg tggaggggag cgtgattatc agaccgacac
    11941 taccgaaaat aatgcctgct atgccaacaa agccccattt aaaaaaatta acccagggat
    12001 tgtttatttc ggcttgttct ggatgccctg gcggataata gacattgact ctgtcacctt
    12061 cgctatcttc ataaaaatca ctaccgatat tcgaattgaa aattagcgtg taatctggcg
    12121 tagggcgaaa ggcaaccact ggataccagg aaccattatc atcgacgtcg tttgagtgat
    12181 tgttgtacca aacggcatca acaatgattc cttctgtatg aaccgcgctt ttggtaaaag
    12241 taaaatggta ataaaagatg attgcagaaa taacgagcaa tatcagaccg ataccgagaa
    12301 aagcgcggga taacacttta ctgtcctgac tcattagcca catccttgct aaataaaaaa
    12361 cgggcggtaa tgccaccgcc cgcttgctga attaacgtcc tgctttcagc ttctgataat
    12421 actcttcata aatgctgctg gctgcgccaa cgtcattctg ccattcacca tttttaatgg
    12481 tttcagcatc cgggtagagc gttttatcgt tcgccacttc cgggcttaac agcttacgcg
    12541 ccgcaaggtt tggcgttgga taaccgatag tttcagcaac ctgttttgcc acatccgggc
    12601 gcagcaggaa gttgatcaat ttcagcgcgc cttctttgtt tttggcattt gccgggatcg
    12661 ccaggctgtc catccagaaa atgccgcctt ctttcggcca caccacgtca attggcgtac
    12721 ccgcctgccg tgcaacgaaa gcagaaccgt tccagatcat gccgaggtta acttcgcctt
    12781 ccatgtacgg gttagctggg ttatcggagt taaacgctgc gacgtttggc atcagttttt
    12841 tcagctcgtt atatgcagct tcaatctctt tcggatcggt ggtgttaccg gagtagccca
    12901 gcttacgcag cgccatctgg aacacttcac gggcatcgtc ggtcaacagc aggctgcctt
    12961 tgtactcagg cttccacaga tcggcccagc tggtgacaga tttcggatcc accgcatcac
    13021 cgttaacacc aatcgccgtc gcaccccaga tatacggaat ggagtaatcg ttattcgggt
    13081 caaacggctt gttgagcatg tctggatcga gattgctgaa gttcgttaac ttcgacttgt
    13141 cgatcttctg gatcatccct tctttacgca ttttatcgac gtaataggtt gaaggaacca
    13201 ccaggtcata ggcaccgtct ttgtacgttt tcagcttggc gtacatggtt tcgttcgact
    13261 cgtaagtcga atagataacc ttaataccgg tttctttggt gaactgttca agcagtcctg
    13321 gcggcacgta ctcggtccag ttgtagaaat acagcgtgtt gttgtcatcg gcgtgagcgg
    13381 cgctcatgcc cagtgccaga gcacccgccg cgagcaggtg gcgtgaccat tttttcattt
    13441 aacgtcccct gtgttacctt tcgttttatc acgagcaata agctggctgg caataaccat
    13501 caccagcgac agcaccagta atatggttgc cagcgcgtta acttccggcg atacgccgac
    13561 tttgaccatc gaataaattt ttaacggcag aatttcataa cttggtccgg tgacgaacga
    13621 agaaactacc acatcgtcca tcgacagggt aaagcttaac acccagcccg ccgccaccgc
    13681 aggcattgcc agtggcagaa tgatttttcg cagaatggta aattcactgg caccgagatc
    13741 tttcgccgct tccagcatcc gcacgtcaaa acctttcagg cgcgaataca ccgtcaccac
    13801 cacaaacggc aggcagaagg tgatatgcga gaacagcagc gaccagaagc caagctgaat
    13861 acccagcagc ataaacagca ccagcagaga aatcgccatc acgatatctg gtgacatcat
    13921 caccacaaac agcattccgc taacgaacgg cttaccacga aagcgataac ggtacagcgc
    13981 aactgccgtc agtgaaccga taagcgtagc aaatgtcgcc gaaaacaccg ccattgttag
    14041 tgaatgctgc gctgcctgta acaggctgtc gttgttcatc agcaggctat accatttggt
    14101 ggtaaaaccc tgccagttga tgccaaagcg cgagctgtta aaggagttca caatcaaaat
    14161 aatgattggg atatacaggt acgcgtagat agcggtcata aaaccgccgc gaagcagtcg
    14221 accgatcatt cgagttccac ctttttattc ataaggcggg acactttcag gattttgaat
    14281 ttattcaatt ttttcctttc tccaaaatct ctccaaaacg tctccccaaa actaggccac
    14341 gcattttaca acactttttt cctgagatgg tgacggatat tccatatggt gtttatcaaa
    14401 taaccaactt aacccattcc tgacctcgag tatcgttata gcgatcggtg gttgcctgga
    14461 ctttatgtcc tagtaatgtt tttgtatcaa taccctgtgc acggtacagc cgttctgata
    14521 gagagcgttg ttcatgaaat gttggcggag tttttcctgc tggtggaatt atcccagcca
    14581 gatcccgtgc tttggcaaag tagtcgctca ggttgtcttt actcatcggc ttcggttgtt
    14641 tctggtgccg actatggatt agatatggac ttaatattct gtctcggcac ccatcaataa
    14701 cttcttttaa cgttatccca atggcatcac agcgtagtgt aagcggtaac gccagacgca
    14761 ttccggtttt tccctgggtg atatgcaagt gttcgttcca cacatctgaa aaacgcatgt
    14821 ggcaaatgtc atcacggcgc tgaccagtaa caatcgcaag aagcattgcg ttacggataa
    14881 agtgtttttc aggcgttgca ttgtaaattt tttgccagtc ttccatggtg agcctggctc
    14941 tggttacttt agggatcggt ttacgggtag cctccggagg attccatcca ggaggaactt
    15001 cccctgcatg ctgtgcttct ttataaatat caacccataa tccacgattt actctcgctg
    15061 tgctgaccat gtctttatcc agccactcat ccagtattaa tgcaaagtct cttacttcca
    15121 gttctttcaa tgggtggttt cccagacggg aaaccaggta tgcagccatt cgggtttttt
    15181 ctttgtgagt tgtagctgca atatctccat ttttcagtcg cgtgtcctgt attttcagat
    15241 atcgatcaac ccatgccttt aatctgatac cccgacgttt tgttgctgac ggactttcat
    15301 caattttgcg catgaaatat tcagcttctg ctgcagctat tcgctgattg gctgtggaag
    15361 cgattttttc tgccttacct ttgtctgttc cgagtccgtg aaattttcca gtcacaggat
    15421 ttttatactg gtagtaaact ctgccagttc tgcgatcaaa cttttcgtaa agaccggcta
    15481 cgtcagtgct gttttttcgt ggcctcggtg acatgagtta aaatctcctt cagtgcatca
    15541 tcatcgccag tatgaatttc cggcgcaatt cccgtttcac caggcccaac aaatactgct
    15601 cggcgatcta tcagccaacg cccacgaatt ttttgtggtc ttggaacgat gtatcctagt
    15661 tttccgtatt tcaccagggt agtgtttgtt attgggagac tgaaccgttt tggtttccac
    15721 tcgtcgagcg ttatcaggta ctgttcgctc atggctatca ctccggaacg cgccagttgc
    15781 agaatatcaa cgacaactgg cgacggttga acattaaaaa tcagcctgat tcgggatcag
    15841 tttttgccag atagctgaaa cgtattttgc ctggtgacg
//
LOCUS       DAZFDP010000113        15659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFDP010000113 DAZFDP010000000
VERSION     DAZFDP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..692)
                     /locus_tag="ACHJ1G_003580"
                     /pseudo
     CDS             complement(1..692)
                     /locus_tag="ACHJ1G_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            693..1430
                     /gene="tagH"
                     /locus_tag="ACHJ1G_003581"
                     /pseudo
     CDS             693..1430
                     /gene="tagH"
                     /locus_tag="ACHJ1G_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHJ1G_003582"
     CDS             1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHJ1G_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4765365.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            1954..3284
                     /gene="tssK"
                     /locus_tag="ACHJ1G_003583"
                     /pseudo
     CDS             1954..3284
                     /gene="tssK"
                     /locus_tag="ACHJ1G_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3289..4050
                     /gene="icmH"
                     /locus_tag="ACHJ1G_003584"
     CDS             3289..4050
                     /gene="icmH"
                     /locus_tag="ACHJ1G_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4765366.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4059..6866
                     /gene="tssH"
                     /locus_tag="ACHJ1G_003585"
                     /gene_synonym="clpV"
     CDS             4059..6866
                     /gene="tssH"
                     /locus_tag="ACHJ1G_003585"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4765367.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESEQDNTGAEPADEAGSEQPEETAETV
                     SPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDL
                     TKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAE
                     LLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDE
                     VEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQ
                     EVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIE
                     PEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGA
                     FTADVEDAQNDESVTKDETVL"
     gene            6863..7606
                     /gene="vasI"
                     /locus_tag="ACHJ1G_003586"
     CDS             6863..7606
                     /gene="vasI"
                     /locus_tag="ACHJ1G_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4765368.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            7611..9023
                     /gene="tssA"
                     /locus_tag="ACHJ1G_003587"
                     /pseudo
     CDS             7611..9023
                     /gene="tssA"
                     /locus_tag="ACHJ1G_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9132..12566
                     /gene="tssM"
                     /locus_tag="ACHJ1G_003588"
     CDS             9132..12566
                     /gene="tssM"
                     /locus_tag="ACHJ1G_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4765369.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            12577..14026
                     /locus_tag="ACHJ1G_003589"
                     /pseudo
     CDS             12577..14026
                     /locus_tag="ACHJ1G_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            13953..14435
                     /locus_tag="ACHJ1G_003590"
     CDS             13953..14435
                     /locus_tag="ACHJ1G_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4765370.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            14479..15393
                     /locus_tag="ACHJ1G_003591"
     CDS             14479..15393
                     /locus_tag="ACHJ1G_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765371.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            15403..>15659
                     /gene="ykfM"
                     /locus_tag="ACHJ1G_003592"
     CDS             15403..>15659
                     /gene="ykfM"
                     /locus_tag="ACHJ1G_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFO4765372.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIISFLIKKTKVV"
BASE COUNT         3686 a         4264 c         4356 g         3353 t
ORIGIN      
        1 ccgtagtgga ccgacacgcg gtggccttca ctggtatggc tgagggtggt taaccacccg
       61 tgctcatcat actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg
      121 tccgacgcat cgtagtgcca gagggtgata agcccctcgt cttcactctg tgtgagtttt
      181 ctggtcaggt catagtggta acgttgcgtc cggccgtcaa aaccgcgctg ttcagtcagc
      241 ctgtcaaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacggtg
      301 atgcgcccgg cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc
      361 ggacgcccgc gtttatcata tgcgatggtg ctgcgtttac cgtccggtga taccgttgct
      421 gtcaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccttctga
      481 ctgaccagtt gcccacgcgg gttataactg ctgtaagtgc tgatgccttc ctcccggtga
      541 acggcgattt gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc
      601 gtaaaggcca gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg
      661 tcctgtatcc ctgtcggcag ctcactcgcc ggccgctttc aggcattgcg gctgtccggc
      721 catcggcacc gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc
      781 tgccgcccat ttatgccagc cccggcgatc gtaatgacga cgtctctgcg gcagaaatgg
      841 cgcaacgcca ccttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga
      901 gcaattccga cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa
      961 taaaaggtct gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc
     1021 cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt
     1081 ttgccgaagg taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc
     1141 gcaatgtccg ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc
     1201 tgctggatgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc
     1261 atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg
     1321 aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc
     1381 aagtctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc
     1441 gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat
     1501 cagtgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga
     1561 aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc
     1621 cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta
     1681 tgaccagata gccagtacgc cgctaccgga tgtgctgggt aaaaaatata tcgaccacca
     1741 ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa
     1801 aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca
     1861 aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag
     1921 cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg
     1981 tggcaggaag ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac
     2041 tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc
     2101 actctcaaca atgaactgct ggcacagggt aagatcatga ttgacagcgc gtcaggcaca
     2161 ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac
     2221 ccgcaacatt ttccggacga gagaagccgc aatatctacc tcgctctgcc ggtcgccagt
     2281 gatgtgagaa atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc
     2341 gatgtccggg atttgcattc agaagaaggt gacacgcgaa cactgacgct gggacaactg
     2401 acgccgcgca tatgagcggt gcagaagata tgagcgccta tattacgctc ccgctttgcc
     2461 gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt atcccctcct
     2521 gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta cagggggcca
     2581 ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag agcggcatcg
     2641 cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc cggtttaccc
     2701 atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt gctgggttgc
     2761 tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg
     2821 atcatcatga tctgaccaaa acatttaaaa cactgttacc ggaagtcaaa cgggcgctgc
     2881 ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac ggtatctggc
     2941 aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg gccgtggcgg
     3001 caaacgtgcc ggtcgatcag atccagcgtc agtttatcca gcagtcgaaa atttcctcgc
     3061 cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg cattccattg cgcgcactga
     3121 tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga
     3181 gcggacaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat gtatccggca
     3241 gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat ggatgagggg
     3301 tcactgtctc tgccaccatt taccggatac gacgaaaaaa gccagcgcga ctatcatctc
     3361 gcgcttcgtg gcaacagcct gaatccgatg atcgacgcag ccacgccgct cctcggtatg
     3421 gtgatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt tgctcaggtg
     3481 gtgacggatg ttcaggccgt ggagcaactg cttcaggagc agggctacga accgggcgtg
     3541 attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct cggtaacggc
     3601 tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca caacgaagcc
     3661 tggggtggag agaaagtttt catcctgctg gagcgcctga tccgtgaacc caaacgttac
     3721 caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg gcgctacaaa
     3781 gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgcctgta ccacgtcctg
     3841 cataaactac gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa aacccagggc
     3901 gggcgttatc agctcatcag tcgcctgacc attaaacata ttttttgtgg tggcgttgtc
     3961 gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac gcaggacatc
     4021 ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt gatcttccca
     4081 cgctggtaaa acggcttaac ctgttctccc gccaggcgct ggagatggcc gcctctgaat
     4141 gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag atgctcgcca
     4201 tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg gaagagttgc
     4261 gccaggcgct gacggtggag aactacacaa ctgcccgttc tgcggacagc tacccggcat
     4321 tttccccgat gctggttgag tggctgaaag agggctggct gctggcgtcg gctgagatgc
     4381 agcacagcga attgcgtggc ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt
     4441 atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt ctgcaacagg
     4501 actttgtgca atggacacag gagtcggcgg aatcagtcgt gccggatgca gacggtaaag
     4561 gcgcaggcac actgacggac gctgctgaca ccctgcttgc ccgctacgcc aaaaacatga
     4621 ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc
     4681 tgatgatcga cattctctgc cgccgccgca agaacaaccc ggtggtggtg ggcgaagcag
     4741 gcgtgggtaa aagcgcctta atcgaagggc tggcgctgcg catcgtggca ggccatgtgc
     4801 cggacaagct gaaaaacacc gatatcatga cccttgactt gggcgcattg caggccggag
     4861 cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcagag gtcatttcct
     4921 ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc gcgggcaacc
     4981 agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc ggcgagctga
     5041 aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa gatgccgccc
     5101 tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa gccaccatta
     5161 ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt gatgatgacg
     5221 ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag ttaccggaca
     5281 aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg tcgtcgccgc
     5341 cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa attcgccagc
     5401 ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc gaggtgctgg
     5461 tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg caccagcagc
     5521 agacgctggt ccgggagatt attgcgctgc gccagcagtt actgggcgtg gcagaggacg
     5581 atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag tcagagtcag
     5641 agtcagagtc agagtcagag tcagagtcag agtcagagtc agagcaggat aataccggtg
     5701 ccgaaccggc tgatgaagct ggcagcgaac agccggaaga gaccgctgaa acagtttccc
     5761 cggtacagcg gctggcccag ctcactgccg aactggacgc cctgcataac gaccggttgc
     5821 tggtctcccc gcacgtcgat aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg
     5881 tgccgctcaa ccgcctatcg cagaatgaaa tgtcggtcat caccgacctg acgaaatggc
     5941 tgggtgacac catcaaaggc caggacctgg cgattgccag cctgcataag catctactga
     6001 ccgcacgcgc cgacctgcgt cgtccgggac gcccgctcgg tgcgttcctg ctggctggcc
     6061 ccagcggtgt gggtaaaacc gaaaccgtcc tgcaactggc agagctgctc tacggcggtc
     6121 gccagtacct gaccaccatc aatatgtccg aattccagga gaaacacacc gtctcgcggc
     6181 tgattggttc gcctccgggc tacgttggct acggtgaagg cggcgtactg accgaagcga
     6241 ttcgccagaa accctactcg gtagtactgc tcgatgaagt ggaaaaagcg cacccggatg
     6301 tgctcaacct gttctaccag gcgttcgata agggcgaaat ggcagacggt gaaggccgcc
     6361 tgattgactg caaaaatatc gtcttcttcc tgacgtccaa cctcggctac caggtaatag
     6421 tcgagcatgc cgatgacccg gaaaccatgc aggaagtact gtatccggtg ctggccgact
     6481 tcttcaaacc tgccctgctg gcgcgtatgg aagtggtgcc gtacctgccg ctgtcgaaag
     6541 agacgctcgc caccatcatt gccgggaaac tggcccgcct ggataacgtg ctgcgcagcc
     6601 gctttggcgc ggaggtgatt atagaaccgg aagtgacgga cgaaatcatg agccgcgtca
     6661 cccgcgcgga aaacggcgcg aggatgctgg aatcggtcat cgacggcaat atgctgccgc
     6721 cgctctccct gctgctgttg cagaaaatgg cggcgaatac ggcggttgcc cggattcggt
     6781 tgtcggcagt ggacggcgca tttacggcag acgtggaaga tgctcagaac gacgagtccg
     6841 tcacaaagga tgaaacggtt ttatgagcag gttgcgcgtt ttccggcaga caggatggtt
     6901 tatcgcgggc ctgatgacgg gcttacccgc cactgccgcg cccgcagaag catcgtcaat
     6961 ggccggtgtg gcggtcgccg tcgccaccac gaccccgccg gatgcgacag ccacgttaca
     7021 ggccatgcag tcctgtcggc gggagtccgc ggcgctggag cggcttgact gctatgaccg
     7081 cctgctggca ccgctgtccc cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt
     7141 gggcgaggcc tggacccgcg ccacagagca ggaaaaacgc cgcgagggca acaccacaga
     7201 actgctggtg acgcaggtgc cgggggagcg cccgacggtg gtgataacca ccccggctat
     7261 cggtcatgtt ccgccacgcc cggtgctgat gttcagttgc gtggacaata tcacccggat
     7321 gcaggtggcg ctgatgcacc ctctggatgt ccatgatatc gccgtcactc tgaatgccga
     7381 caaccgggca ttgcgcagcc actggtttgt gcgcgagaac ggcaccctgc tggagtccag
     7441 ccgggggtta tccgggattg atgaaattaa gcaactgttc ggcgcaaaaa cgctgacggt
     7501 ggataccggc gcagacaatg ccgccggaaa gctgactttt aacattgacg ggctggcaag
     7561 ggcgattgct cccctgcgcg atgcctgcca ctgggcggga gaataaaaat atggcaatgg
     7621 acttacgcga tccgaatgtc tggatatcgc acctgctgga aaacctgccg gaagagaaac
     7681 tggcatcggc gctgaaagat gacaacccga actgggagta tatcgacggc gaaatcgtca
     7741 agctgggatc gctggcccac agtcaactgg atatcccgga actccagcgc cgtgggctgg
     7801 tcattctggc ttcggagagt aaggattttc gtctgctggc gcatctgctg agaaccctgc
     7861 aacacgccgg tgatcctcat ctcgcattgc gcctgctggc gctgtatgtg gagcattact
     7921 gggcggtggc tgcgccgcag aatatggcgc ataaaaaacg ctttgccagc caggtcatta
     7981 aacgctttga aacaggtatt gaagtttttt cacagaacgc cgccacagcg cagcgcgatg
     8041 cgctgtcagg ggaactggca aaactggcgc aatgctggca gtcacataac gccccggaac
     8101 tggcacaggc tactgatgat ctttttgccc tgtaccagcg tgcgtttaat cgtgcggcac
     8161 cggctcctgt ccccactccg gcggcctccg gtagttcacc acagaccacc gccacgtctg
     8221 aaagcggcgt gacgcaaccc agtgctccgg ctccccaaat cgtcatcgac agtcacgacg
     8281 acaaagccta gcgcgacacg ctgttaaaag tggcggccat tttatgtgaa cgccagccgg
     8341 attcaccgca gggctaccgt ctgcgccgcc atgcgctttg gcagaacatc accagtacgc
     8401 cccaggcgga aagcgatggc cgtactccac tggctgcggt ctccgccgat atggtggcag
     8461 attaccatgc tcagctcggt agtgccgata tggcgctgtg gcagcaggtg gagaaaagcg
     8521 tactgctggc tccttactgg ctggatggtc actgcctttc tgcacagacg gcgctgcgtc
     8581 tgggttacaa acaggtggca gacgccatcc gcgatgaggt catccgcttc cttgagcgtc
     8641 tgccccagct taccgggctg ctgtttaatg accacacacc gtttatcagt gagcagacga
     8701 aacaatggct ggcagcttcg cccgacgcca aagttgcacc ggttgcgcaa atcggtgagg
     8761 aatcgaaggc agccagagcc tgttttgctg agcagggtct ggaggcggcg ctgcgatatc
     8821 tggacatgct acccgaaggc gatccccgcg atcagtttca ccgccagtac cttgccgcac
     8881 agttgacgga agaggcgggg ctggtacagc ttgcgcagca gcagtaccgg atgttgttca
     8941 ggatgggatt gcagatgatg gtggcagact gggagccgtc cctgcttgag cagctagaac
     9001 aaaaattcac ggcagaacaa taaaagagaa caggagtttt tgtgttcaga ttaccaacac
     9061 cccgactact cagcggactc aaatcagccc tgcgaccggc gatgccccgg tttaaagtct
     9121 ctgctttctg gctgctgata ctggcgtgga tttttctgct ggtgtggatc tggtggaaag
     9181 gcccgatgtg gacgctgtat gaagaacagt ggctcaaacc actggcgaac cgctggctgg
     9241 caacggcggc gtgggggatt attgccctgg tgtggcttac cgtccgggtg atgaagcgcc
     9301 tgcaacagct tgaaaaaatg caaaagcaac agcgcgagga agccgttgat ccgctcagcg
     9361 tggaactgaa cgcccagcag cgttatcttg accgctggct gctgcgcctg caacgccatc
     9421 tcgacaaccg ccgtttcctg tggcagttgc cgtggtatat ggtcatcggc ccggcgggca
     9481 gtggcaaaac gacactgctg cgcgaggggt ttccgtccga cattatttat gccccggagg
     9541 gcgcacgcgg cgcagaacaa cgcctgtacc tcacgcccca tgtcggtaaa caggcggtga
     9601 tctttgatat cgacggcaca ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc
     9661 tgtgggaaca tgccctcggc tggctgaagg aaaagcgcgc acgccagccg ctgaacggga
     9721 ttattctgac actcgattta cccgatctgc tgaccgcaga caaacgccgc cgcgagcatc
     9781 tgttacagac gctgcgcagc cgcttacagg atatacgcca gcatcttcac tgccagttac
     9841 cggtttacgt ggtactgacc cggcttgatt tattacaggg ttttgccgcc ctgttccagt
     9901 cactcaacag acaggatcgc gatgcgattc tgggcgtcac gttcacccgc cgcgcccatg
     9961 aaaatgatga ctggcgaaca gagttgaatg ctttctggca gacatgggtg gatcgaatga
    10021 atctggcgtt gccggatctg atggtcgctc agactcacac ccgcgcgtct ttattcagtt
    10081 tttcccgcca gatgcaggga agccgtgaac cgctggtgtc actgcttgag ggtctgcttg
    10141 atggcgaaaa tatgaacgtg atgctgcgtg gtgtctatct cacctcttcg cttcagcgtg
    10201 ggcagatgga tgatatattc acccagtctg ccgcccgcca gtaccggctg ggcaataacc
    10261 cactggcgtc ctggcccctg gtggacaccg cgccttattt cacccgcagc ctgttcccgc
    10321 aggcattact cgcagagcct aatctggcaa cagagagccg cgcctggctg atacgttccc
    10381 gtcgccgcct gacggttttc tctgccacag gcggcgtggc agcactgctg ctcatcaccg
    10441 gctggcatca ctattacaac ggtaactatc agtccggcat caccgtgctt aagcaggcca
    10501 aagcctttat ggacgtgccg cctccgcagg gggaagatga ctttggcaac ctgcaactgc
    10561 cgctcctgaa cccggtacgc gatgccacac tggcctatgg cgactggggc gaccgcagcc
    10621 gtctggccga tatgggactg taccagggac gacgtatcgg gccttatgtg gaacagacct
    10681 atctgcaact gctggagcaa cgttacctgc cctcgctgtt taacgggctg gtcaaagcga
    10741 tgaacgccgc gccgccggag agtgaagaaa aactcgcggt gctgcgcgta atgcgaatgc
    10801 tggaggacaa aagcggacgt aacaatgagg tggtgaagca gtatatggca aaacgctgga
    10861 gcgaaaaatt ccacggccag cgcgatatcc aggcacaact gatgtcccat cttgactacg
    10921 cgctggctca tactgactgg catgcagagc gtcaggcggg cgacggtgac gccatcagcc
    10981 gctggacgcc atatgacaag cccgtggtat cagcacagaa agaactgagc aaactgcctg
    11041 tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct gggcgttctt cctgccgacc
    11101 tcaatctgcg tgaccaggta gggccaacct ttgaccaggt gtttacatct gccgatgaca
    11161 acaaactggt tgttccacag tttatcaccc gctacggcct gcaaagctat tttgtaaaac
    11221 agcgcgatga actggttgaa ctgacagcga tggattcctg ggtacttaac ctcacccgca
    11281 atgtgaaata cagtgacgcc gaccgtgcgg aaatccagca ccagttgacc gagcagtata
    11341 tcagcgacta caccgccacc tggcgggctg ggatggacaa tctgaatatc cgcaattttg
    11401 agtccatcgg acaactgacc ggggcgctgg agcaggttat cagcggcgac cagcctttgc
    11461 agcgggcgct gaccgtgctg cgtgacaaca cacagccagg cgtcttttct gaaaaactct
    11521 ctgccaaaga acgggaggaa gccctggcag agccggatta ccagttactc acccgcctcg
    11581 ggcatgaatt cgccccggaa aacagtaccc tggcagtaca gaaagacaaa gaaagcacga
    11641 tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta cctgctggca atccagaacg
    11701 cgcctgtacc agggaaatcg gcgctgaaag ccgtgcagtt acggcttgat cagaacagca
    11761 gcgatccgat attcgccacc cgccagatgg caaaaacgct gcctgctccg ctcaaccgct
    11821 gggttggcag gctggctgac caggcctggc atgtggtgat ggtggaggct gttcattata
    11881 tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa cgagcaactg gcaaataact
    11941 atccgtttaa tccgcgttct gcacaggatg cctcactgga tgccttcgaa cgcttcttta
    12001 aaccggatgg catactggat accttctacc agcagaacct gaagctgttt atcgataatg
    12061 acctgagtct ggaggatggc gataacaacg tcattattcg cgaagatatt attgcgcaac
    12121 tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa acagaacggt ctgggaacat
    12181 cctttgccgt ggaaacggta tcgctttcag gcaataaacg ccgcagtgta ctgaaccttg
    12241 acggtcagtt agtcgattac agccagggcc gtaactatac cgcccatctg gtctggccta
    12301 acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc
    12361 cgcgcagtat cagcttcagc gggccgtggg cgcagttccg cctgttcggg gccggacaac
    12421 tgaccggagt acaggatggc aactttaccg tgcgctttag cgtggacggt ggcgcgatga
    12481 cctaccgtgt gcataccgac acggaagata acccgttcag cggtgggttg ttcagccagt
    12541 ttggtctgtc agacacactg tactgaggag gcagggatga atagtaacgt actgacacaa
    12601 actatcgtta ccggcagtga cccgcgcggg ctgccggaat tcagcgccat ccgcgaggaa
    12661 ataaacaaag ccagccaccc gtcacagcct gagctgaact ggaaactggt ggagtcgctg
    12721 gcgctggcga tttttaaagc caacggtgtg gatttacaca ccgccaccta ctatacgctt
    12781 gcccggacac ggacacaggg actggcggga ttctgcgaag gtgcggaact gctggcggca
    12841 atggtaagcc acgactggga taagttctgg ccgcagggcg gcccggcgcg tactgaaatg
    12901 ctggactggt ttaactcccg aaccggcaat attctgcgtc agcaaatctc ctttgcggaa
    12961 tccgacctac cactgatcta ccgcacagag cgggcattgc agcttatctg cgataagctc
    13021 cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc tgctctattt tatgcagaac
    13081 acgcgtaaac ggcttggacc ccagctgaag agtaacactg agaacgccgc acagaccacg
    13141 gtcagaacgc tgatttatgc cccggaaaca caggcatctt ccacaccaga agcggtagtg
    13201 cctcccctgc ccggcctgcc tgagatgaaa gtggaagttc gcagtctgac agagaatccc
    13261 ccacaggcca gtgtgataaa tcaaggcagt acggtaagag ggtttatcgc agggatcgcc
    13321 tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg tctatccggt gcagcagcaa
    13381 ctgttacagg ttaacgacac cgctcagggc gcagcaacgg tgtggatggc ctcacctgaa
    13441 ctcgaaaact atgagcgcag gctgcaacaa cttcttgata cctccccggt acagccgctg
    13501 gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag
    13561 caacaggcct cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag tagcccgcag
    13621 ttgcgtggct ggttgcagac ccgccaggac ttacatgctt ttgcagatct agtgatgcag
    13681 cgcgagaaag agggactaac cctttcctat atcaaaaatg tcatctggca ggcggagcgg
    13741 ggactggggc aggaaacacc cgttgagtct ctgttgacgc agtaccatga tgcccgtgcg
    13801 cagaagcaga atacagatgc gctggaaaaa caaattaatg agcgactcga aggcgtgtta
    13861 agccgctggc tgctgctgaa gaataacgtc atgccagagg cggcaaccgg taccacggcc
    13921 gaaaaataat tatttaatta aaggagatta tcatggcaaa tataagttat ttatctttga
    13981 gtggagagac acaagggcta atttcagcag gttgctcaac gcttgattct gtcggaaaca
    14041 aagcgcagcc agaacataaa gatcagatca tggtttatgc gttaatgcat tccatctcaa
    14101 gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa accggttgat aaatcctcac
    14161 cattgctggc aaaagcactt agtgacaatg aaaaaatggc aatatgtgaa tttatactct
    14221 accgaaccag taaagcgggt atttaccagc cttattataa aataaatctg tcaaaagccc
    14281 gaatatcaag tattgatttt gtcacaccgc atgctgtcct ggaaaaagaa ctggaaccac
    14341 aggagagaat tgcctttatt tatgaagata tttcctggga gcatacgtta gccggtacga
    14401 atgcaatgag taaatggcag gatcgtgtac agtaacttac tgtaataaca taataaatta
    14461 ctattgcgga gagataaaat ggctggttta aggaataaca acaacacaca gaatgcacag
    14521 tgggctgatt atgtgggtga cattcttcgg ggggctcagc cgataaacca gctagtacct
    14581 cagcaccctt atcttaacga cgtcccactc attgatgagt taaggcatca gaacactcat
    14641 catgttattc tcttgacact tgatgttgca aaaaaaatac tcagcccaat aacatcattt
    14701 gattacattc attttatcac tacacatcct tcaggtataa aagatacact cgcctggtta
    14761 gttaacgcgg gtaaattaat gactgagttt gatgacaatg ggaaaattat atttaacctt
    14821 aatgccttaa agtatacaaa agcaagttac tttgaaatac ttggagagaa atatataaaa
    14881 atcacaacat ccagcccctg gcttttagaa aagctcggta aatatatttt ctcttcacga
    14941 gcgcctcaag ttctggaact cgctattggc tggcgaggag ctttatctga atcaataaaa
    15001 ggagttaagt tctgtatctg gttttcagta gcctggagaa ccatagaatt tattatgagt
    15061 tcagagcgag atctggttaa ttttcttggc gatttttcaa tggatgtggc caaagcagtt
    15121 atagccggtg gtgttgcaac cgctattgga agcctggctt cttttgcctg tgttagcttt
    15181 ggctttccag taattcttgt cggaggagca attttactga cagggatagt gtgtacagtt
    15241 gttttaaatg aaatcgatgc tcaatgccat ttatcagaaa aattaaaata tgcaattaga
    15301 gatggactaa aacggcaaca ggaacttgat aaatggaaaa gggaaaacat gactccattt
    15361 atgtatgttc ttaacactcc acccgtgata tgaggattaa ccatgcgttt acatgttaaa
    15421 ttaaaagagt ttctttcgat gttttttatg gccatattat ttttccctgc attcaatgct
    15481 tctttgtttt ttaccggagt taaacctcta tattcaataa taaaatgctc cacggaaata
    15541 ttttatgatt ggagaatgtt gattctgtgc tttggtttca tgtcgttttc ctttttaaat
    15601 attcatgtaa ttttacttac gataataata tcattcttaa tcaaaaaaac gaaggtggt
//
LOCUS       DAZFDP010000114        15543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFDP010000114 DAZFDP010000000
VERSION     DAZFDP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..353
                     /gene="yjbE"
                     /locus_tag="ACHJ1G_003593"
     CDS             111..353
                     /gene="yjbE"
                     /locus_tag="ACHJ1G_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4765373.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            467..1105
                     /locus_tag="ACHJ1G_003594"
     CDS             467..1105
                     /locus_tag="ACHJ1G_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4765374.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1102..1839
                     /locus_tag="ACHJ1G_003595"
     CDS             1102..1839
                     /locus_tag="ACHJ1G_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4765375.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1839..3935
                     /locus_tag="ACHJ1G_003596"
     CDS             1839..3935
                     /locus_tag="ACHJ1G_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4765376.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3982..4260)
                     /locus_tag="ACHJ1G_003597"
     CDS             complement(3982..4260)
                     /locus_tag="ACHJ1G_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765377.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4261..4404
                     /locus_tag="ACHJ1G_003598"
     CDS             4261..4404
                     /locus_tag="ACHJ1G_003598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765378.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4530..4940
                     /gene="psiE"
                     /locus_tag="ACHJ1G_003599"
     CDS             4530..4940
                     /gene="psiE"
                     /locus_tag="ACHJ1G_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4765379.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4984..6459)
                     /gene="xylE"
                     /locus_tag="ACHJ1G_003600"
     CDS             complement(4984..6459)
                     /gene="xylE"
                     /locus_tag="ACHJ1G_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO4765380.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6831..7721)
                     /gene="malG"
                     /locus_tag="ACHJ1G_003601"
     CDS             complement(6831..7721)
                     /gene="malG"
                     /locus_tag="ACHJ1G_003601"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4765381.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7736..9280)
                     /gene="malF"
                     /locus_tag="ACHJ1G_003602"
     CDS             complement(7736..9280)
                     /gene="malF"
                     /locus_tag="ACHJ1G_003602"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4765382.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9434..10624)
                     /gene="malE"
                     /locus_tag="ACHJ1G_003603"
     CDS             complement(9434..10624)
                     /gene="malE"
                     /locus_tag="ACHJ1G_003603"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4765383.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10989..12104
                     /gene="malK"
                     /locus_tag="ACHJ1G_003604"
     CDS             10989..12104
                     /gene="malK"
                     /locus_tag="ACHJ1G_003604"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4765384.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12176..13516
                     /gene="lamB"
                     /locus_tag="ACHJ1G_003605"
     CDS             12176..13516
                     /gene="lamB"
                     /locus_tag="ACHJ1G_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4765385.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13759..14679
                     /gene="malM"
                     /locus_tag="ACHJ1G_003606"
     CDS             13759..14679
                     /gene="malM"
                     /locus_tag="ACHJ1G_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4765386.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14908..15543
                     /locus_tag="ACHJ1G_003607"
                     /pseudo
     CDS             14908..15543
                     /locus_tag="ACHJ1G_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3902 a         3935 c         4046 g         3660 t
ORIGIN      
        1 gccatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac tcgatcccgc
       61 ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt atgaaaaaag
      121 ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg gctgcacctg
      181 tacaggtggg cgaagcggca gggtcggcag caacgtcggt ttcggcgggg agttcctccg
      241 ctaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca accggtggcg
      301 gtgatggttc taataccggg accaccacaa ccacgaccac cagtacccag taataaagta
      361 tgtatcccca aaataatttg agtcattgca tctgtggctt gaagtatgaa gggatttacc
      421 ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga agcgacctgc
      481 actcattctt atctgcctgc tattacaggc ctgttcagcc acgactaaag agctgggcaa
      541 ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag ctgacggacg atgatattca
      601 aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcaat tgtttgtggt
      661 gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcaag ccactcttgt
      721 cacacaacat ggtcgtctgg tgaagacttt gcttggcggc gacaacctga ttgaagtgaa
      781 taaccttgcc gccgacccgc tgattaaacc cgcacaaatt gttgatggtg caacatggac
      841 gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcac gctcagtctt
      901 caaatgggat ggcaccgata ccgtcaaagt cggcagcgat gaaaccctgg ttcgcgtgct
      961 ggacgaagaa gtctccaccg accaggcgcg ctggcataac cgctactgga tcgacagcga
     1021 agggcaaatt cgccagtcgg aacagtatct cggcgcggat tatttcccgg tgaaaaccac
     1081 tctcatcaag gcggcaaaac aatgattaaa caaacttttg tcgcgttgct tttgagtgtg
     1141 ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg cagcagtgag
     1201 gccaaaacac taacaggcgc agagcattta atcgatctgg taggccaacc gcggctggca
     1261 aacagctggt ggcccggtgc ggtgattagc gaagagctgg caacggcggc agcattgcgt
     1321 cagcagcagg cgttgctgac acggctggca gaacagggcg cagattccag caccgacgat
     1381 gccgccgcca ttaacgcctt acgccagcaa atccaggcat tgaaagtgac aggcaggcaa
     1441 aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc gccgttgcag
     1501 ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt cgggcttatc
     1561 agccatcctg gcaatcagcc attcactcca ggtcgcgacg tggcgagcta tctctccggg
     1621 cagaacctgc ttagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc ggacggacgc
     1681 acgcaaaaag cgccggtggc ttactggaac aagcgtcacg tagagccgat gcccggcagc
     1741 attatttatg ttggcctcgc ggactccgtc tggagtgaga cgcctgatgc ccttaacgcc
     1801 gacattcttc agactctgac gcagcggata ccccaataat gaaaaaaaga catctgctta
     1861 gcttactggc gctgggcatt agcatggctt gctacggcga aacatatcct gcgcccattg
     1921 gcccgtcgca gtcggatttc ggtggcgtag gattattaca aactcccacc gcgcgtatgg
     1981 cacgggaagg ggagttgagt ctgaactatc gcgataacga tcagtaccgt tattactcag
     2041 cttcagtgca actcttcccg tggctggaaa caacgctgcg ctacaccgac gtgcgcaccc
     2101 ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat aaagccttcg
     2161 atctcaaact gcgtttgtgg gaagagagtt attggctgcc gcaagtggcg gttggtgcgc
     2221 gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc aaagcctggg
     2281 ggccgttcga ttttacgctc ggcctgggct ggggatatct gggcaccagc ggtaatgtga
     2341 aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc tacaaacagg
     2401 cgggatctat cgacggtagc cagatgttcc acggtcctgc ctcactgttt ggcggcgtgg
     2461 aataccagac gccctggcaa ccgctgcgcc tgaaactgga gtatgaaggc aataattatc
     2521 agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggt gcgatttatc
     2581 gcgttaccga ttgggccgac gttaacctta gctatgaacg tggcaacacc tttatgtttg
     2641 gcgttacgtt gcgcaccaac tttaacgatc tgcgcccgtc ttacaacgat aacgcccgcc
     2701 cgcaatatca accgcagccg caggatgcca ttttgcagca ttcggtggtg gcgaatcagt
     2761 taacgttgct gaaatacaat gccggacttg ccgatccgca gatccaggcg aaaggcgata
     2821 cgctgtatgt taccggcgag caggtgaaat atcgtgattc gcgcgaaggg atcatccgtg
     2881 cgaatcggat cgtgatgaac gatctgccgg atgggatcaa aacgatccgc attacagaaa
     2941 atcgccttaa catgccgcag gtgacgacgg aaaccgatgt cgccagcttg aaaaatcatc
     3001 tcgccgggga gccgctgggc catgaaacga aggtggcgca aaaacgcgtc gagccagtgg
     3061 ttccgaagtc caccgagcag ggatggtata tcgacaaatc acgctttgat ttccatatcg
     3121 atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat cagctgggcg
     3181 tgatgggaac ggcagatttg tggctgacgg accatctgct gaccaccggc agcctgtttg
     3241 caaatcttgc caacaactac gacaagttta actacaccaa tccgccgcag gactcgcact
     3301 taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa cgatgtctat gtgaataacc
     3361 tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc tatggaggtt
     3421 atctcgaaac catgtttggc ggtgccgggg cagaagtgtt gtatcgtccg ctggacagca
     3481 actgggcgtt tggtctggat gccaactacg ttaaacagcg cgactggcgt agtgcaaaag
     3541 atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcc tactggacgc
     3601 catctttcgc ccaggacgtg ttggtgaaag cgagcgtcgg gcagtatctg gcaggggata
     3661 aaggcggcac gctggagatc gccaaacgct ttgatagcgg cgtggtggtt ggcggctatg
     3721 ccacgatcac gaatgtttcg aaagaggagt acggcgaagg ggacttcacc aaaggcgtgt
     3781 atgtctctgt gccgttggat ctcttctcgt ctggcccgac acgcagccgt gcggcgattg
     3841 gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc cagttgtatg
     3901 acatgaccag cgaccgtagc gtcaatttcc gctaagtcat gggaaaggtg ccagttttcg
     3961 caatcactgg caccctcatt cttaatggcc tgttgagcca tcaggaacgc agttcacccc
     4021 cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg tcgatatcgg
     4081 ttgcagatct ggttgcctga gcgactgggc catcattggg ctggagacat tgctttgcgg
     4141 taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa cagagactga
     4201 taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt tacgtttcat
     4261 ttgttccaga ggaacattgt cgatttttcg cgcattgctg gtggctggga atcacctgaa
     4321 tgggtgattt ttgaattacc ggctttggtg cggtttgtct tgccggatgc gccgccaggc
     4381 gcggcttatc cggcctacgg gtaggtatat ccggctttgg tggaggcgcg ctccaaatcc
     4441 aggttgaaca aaacatacac aaaaaatata gatctccgtc acatttttgc gttatacagg
     4501 aagctcgcca ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg cgcgtggagt
     4561 ttatctccac cattttgcag accgtactca atcttggcct gttgtgcctc ggcctgattt
     4621 tggttgtctt cctcggcaaa gaaacggtgc atctggctga tgtgctgttc gcgccagaac
     4681 aaaccagcaa atatgagctg gtagaagggc tggtggttta ctttctctat ttcgaattta
     4741 tcgcgctgat tgtgaagtac tttcagtccg gttttcactt cccgctacgt tactttgtct
     4801 atattgggat caccgccatt gtgcgcttga tcatcgtcga tcataaatcg ccactggatg
     4861 tattgatcta ctcggcagcg atcctgttac tggtgatcac cctgtggctg tgtaattcga
     4921 aacggctgaa gcgggagtaa aaagacagca cgccgaactg gcgcggcgtg ctggacagga
     4981 agattacagt gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga gggcttccag
     5041 ctcctcaagg gttttacctt tggtttccgg gacaaatttc cacataaata gtgctgccag
     5101 aacgcccata caaccgtaaa tccagtagga gaaaccgttg tggaaatggg tcaccagcca
     5161 ggagtttttg tccatcatcg ggaaggtcca ggagacgaag tagttcgcca gccactgggc
     5221 cgccaccgcg attgccagcg ctttaccacg aatagcattc gggaagattt ccgacagcag
     5281 tacccagcat accggacccc aggacatggc aaaggcagca acatagaaca gcatcgacag
     5341 tagcgccaca atacccggtg cctgagtgta aaacgcggta ccgaggctaa acataccgat
     5401 tgccattccg agcgcgccga taatttgcag tggcttacga ccaaatttat ccaccgtcat
     5461 aattgccaga acggtgaagg tgaggttgat aactccgaca ataatggtct gcaacagcgc
     5521 gatatccgtg ctggccccca gcgttttgaa cacttccggc gcgtagtaca acaccacatt
     5581 gatgccgaca aattgctgga agatggagag cataacgccg ataacaatca cgcccacgcc
     5641 gaacatcagc agacgaccac cggttttgcg tccatgatcc agggagtgtt taatttcctg
     5701 caccgcctgg gctgccaaag cgttgcccat aattttgcgc aggatgcctt ccgcttgttc
     5761 ttgcttaccg cgtgacatca gccagcgagg gctttccggc acggtataaa gcagcattaa
     5821 gaacagcagc gcagggatac attccgaagc aaacatataa cgccagccgt cagtattcag
     5881 ccaggtggca tcgccggaac gggcaataaa atagtttacg cagtaaacta aaagttggcc
     5941 gaaaataatc gcaaactggt taaaagagac cagcttccca cgaatatgtg caggagccag
     6001 ttccgcaata tacattggtg agagcattga ggctaaacca acaccaatac cgccaataat
     6061 gcgataaata acaaattccg ggacataacc tgccagataa acaggcactg tgttgtccgg
     6121 gtttatagag gtaaaaccaa gttctggcca ggcagaacct acaccagaaa taaaaaacag
     6181 gacagcagca atcttaagtg aatcacgacg accgaagcgg ttactgcaat aaccaccgag
     6241 ggcaccgccg atgatgcaac caatcagagc gctggctacg caaaacccta acagggagtt
     6301 ggcagcggat tcacttaagt tttgtggagc aacaaagacg gtatggagtg actcaacagt
     6361 accggaaata acggcggtgt cgtagccaaa taataaacca cctaatgtag cgactaaggt
     6421 aatcgaaaat atataactgg aattatactg ggtattcatt cagacctgcc ttagaccatt
     6481 ctgatctttt atcagtgcat tgatgttgga attgtcaggc agttaaggca attatcattt
     6541 tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat tatctaaaat
     6601 tgatgaaaac aatactattg ccgtgactca gagcacgaaa gagaattatc gtaagtggga
     6661 aaacaaataa cgtaaaaata ataagctcta tgatgggaat attaaccggc gaacgattca
     6721 gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc gaataacagt cacaagttgg
     6781 gataacgtaa gttgagggtg cagcggcata acattggcag aacaacatct ttaacctttc
     6841 acaccacctg ccgtcaggcc gttgaccagc cagcgttgag ccagcaagaa gacgatggtg
     6901 atcggtaatg cagacatcac ggcagcggcg gcaaagtcac cccacaggta gttttgcggg
     6961 ttgaggtatt gctgcatccc cacggccagg gtgtagctgt ttacgtcacg cagtaacagc
     7021 gacgcgaccg gaacttcagt aatggcagcg ataaacgaca agatgaatac caccgccaga
     7081 atcggtactg acagcggcaa caggacaagg cggaaggcct gccacggtgt cgcaccatcc
     7141 agcgcagccg cttcttccag cgaactatcg atggtttcga aatagccttt gatggtccag
     7201 acatgcagcg caatcccacc cagatacgcg aaaattacgc cgccgtgagt attcaggcca
     7261 atgaatggaa tgtactcacc cagacgatca aacaacgcat acaacgcgac cagtgaaagt
     7321 actgccggga acatctggaa aatcagcatt cctttcagca gcgtcgcttt gcctggaaag
     7381 cgcatacggg cgaaagcgta ggcgcaggtg gtagagagcg ccacaatgcc aatcgcggaa
     7441 atcccggcga cctttaccga gttccacagc cacagcagta ccgggaatgg cggtggcgta
     7501 atgcgaccat cagcctgttc aacgctaaaa cctaacgcca gtttccagtg atcccaggag
     7561 atttgctccg ggatcaggct accggtcgcg aagtttccct gacgcagcga gatagcgacg
     7621 accatcagca gcgggaacat aatcgccgcg ataaaaagta gcagtagcag gtgagtaata
     7681 aacaaacgtg ctttttgcga tttcggttgg accattgcca tttttgttat ctcccttaat
     7741 caaacttcat tcgcgtggct ttcaggttca ctatcgccag cgcacccacc agcaggaaga
     7801 tcagcgtggc aattgctgct gccagaccga agtcctgacc cccgccgcct tcaaaagcga
     7861 tgcggtaggt gtagttaaca agcaggtcgg tataaccggc tggcgtggtc gtgccaagac
     7921 gatccgggcc gccgttggtt aacagttgaa tcagcacgaa gttgttaaag ttaaaggcga
     7981 agctggcgat catcagcggc gtcagcggtt taatcagcag cggcagcgta atcttaaaga
     8041 agttctggaa cggacctgcg ccatccattg ctgaggcttc atacaaatcg tccggaatcg
     8101 ctttcagcaa gcccatgcag aggatcatca tgtacggata acccagccag gtattgacga
     8161 taatcagcat cgtgcgggcg gtggtcggat cgctgaacca ggcgggcttc acgccaaaca
     8221 gcgcgctcaa catcatgttg atttcaccga agctctggtt aaacaaccct ttgaaaatca
     8281 agattgaaat gaacgatggc accgcgtagg gcagaatcag caggacgcga tagaccgctt
     8341 tgccgcgcaa cgcttcccac tgcaccagac acgccagaac catgccgacc gccaccgtta
     8401 aaaagacagt gatcagcgag aacaccacgg tccagacgaa aatggcgagg aacggtttct
     8461 gaatgccttc gtcggtgaag acgcgggtaa agtttttcca gccagtagtc acggtgtaac
     8521 cggggcttag cttttcatcg ccccagttgc cgtcggcggt aatggactgg taaaagccaa
     8581 tttggttatt cggacgatat ttcacgccgc tctgattatt cgtcaacgtg ccgtcaccgt
     8641 cgagtgtgta gagcggctgc gtgccagaaa actggcgcag ggagctcatc atcactttgt
     8701 tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg acgattctgg gtaatcacgc
     8761 gcagattcgc gcgttcgcct tcgggctggg tggtcgtttc tttcagttgc agtttttgct
     8821 cgccgccaaa tttaaaagcg tcggagaggt aatttttgcc ggtttcgccg tcgctgaggg
     8881 ctagttgcca ctcatcgccc gccgggtaaa gaccaaaatt ataggttttg cctgcttgcc
     8941 aggagcgatc tagcaacact tcctgcgcac gttcaaaagt cagctggtta gtgctgctgt
     9001 agttggtgaa ggcaatggcg atggtgcaga ccagagggaa gaggacgaat aatcccattc
     9061 cagccattcc cgggtaaaca tagcgccagg cgtaggcttt acgattggcg aaaatataca
     9121 gccccgctga actcaatatc agcgtggtaa tggcgaacag gtattcccct tgtgcgtaca
     9181 ttaaaacaac aaggtaaccc accagcaggc cgagcagacc taacactgac catttcagcg
     9241 cgtcgctttg ccaccaatgt ttctttttaa tgacatccat ggggttcttc ctcattccag
     9301 gacggataag gctttcacgc cttatccgac aacaactgcc tgatgcgacg ctgacgcgtc
     9361 ttatcaggcc tacatacgtt tcggttttgt aggccggaca aggcgttcac gccgcatccg
     9421 gcatttcaca gcattacttg gtgatacgag tctgcgcgtc tttcagggct tcatcgacag
     9481 tctgacgacc gctggcggcg ttgatcaccg cagtacgcac ggcataccag aaagcggaca
     9541 tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatggtg gctgcaatac
     9601 gtggatcttt cgccaactct tcctcgtaag acttcagcgc tacggcaccc agcggtttgt
     9661 ctttattaac cgcttccaga ccttcgtcag tcagcagata gttttcgagg aactctttcg
     9721 ccagctcttt gttcggactg gcggcgttaa taccggcgct cagcacgcca acgaacggtt
     9781 tagatggttg acccttgaag gtcggcagta ccgttacacc ataattcact ttgctggtgt
     9841 cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct ttattaaagg
     9901 cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta atcaggtcaa
     9961 ccaggaaggt cagacccgct ttcgcgccag cgttatccac gcccacgtct ttaatgtcgt
    10021 atttgccatt ttcatacttg aacgcataac ccccgtcagc agcaatcagc ggccaggtga
    10081 agtacggttc ttgcaggttg aacatcagcg cgctcttacc tttcgctttc agttctttat
    10141 ccagcgccgg gatctcttcc caggtttttg gtgggttcgg cagcagatct ttgttataaa
    10201 tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag cttgccgttg taacgtacgg
    10261 catcccaggt aaacggatac agcttgtcct ggaacgcttt gtccggggtg atttcagcca
    10321 acaggccaga ttgagcgtag ccaccaaaac ggtcgtgcgc ccagaagata atgtcagggc
    10381 catcgccagt tgccgcaacc tgagggaatt tctcttccag tttatccgga tgctcaacgg
    10441 tgactttaat tccggtatct ttctcgaatt tcttaccgac ttcagcgaga ccgttatagc
    10501 ctttatcgcc gttaatccag attaccagtt taccttcttc gattttggcg agagccgagg
    10561 cggaaaacat catcgtcgtt aatgcggata atgcgaggat gcgtgcacct gtttttattt
    10621 tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa cacctaaacg gactctagtt
    10681 tctttatacg gcaacctctt tccatcctcc ttgcccctac gccccaccgt tgctttgtgt
    10741 gatctctgtt acagaattgg cggtaatgtg gagatgcgca cataaaatcg ccacgatttt
    10801 tgcaagcaac atcacgaaat tccttacatg acctcggttt agttcacaga agccgtgttc
    10861 tcatcctccc gcctcctccc ccataaaaaa gccagggggt ggaggattta agccatctcc
    10921 tgatgacgca tagtcagccc atcatgaatg ttgctgtcga tgacaggttg ttacaaaggg
    10981 agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa agcctggggc gaggtcgtgg
    11041 tatcgaaaga tatcaatctc gatatccatg aaggtgaatt cgtggtgttt gtcggaccgt
    11101 ctggctgcgg taaatcgact ttactgcgca tgattgccgg gcttgagacg atcaccagcg
    11161 gcgacctgtt catcggtgag aaacggatga atgacactcc gccagcagaa cgcggcgttg
    11221 gtatggtgtt tcagtcttac gcgctctatc cccacctgtc agtagcagaa aacatgtcat
    11281 ttggcctgaa actggctggc gcaaaaaaag aggtgattaa ccaacgcgtt aaccaggtgg
    11341 cggaagtgct gcaactggcg catttgctgg atcgcaaacc gaaagcgctc tctggcggtc
    11401 agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga gccaagcgta tttttgctcg
    11461 atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca aatgcgtatc gaaatctccc
    11521 gtctgcataa acgcctgggc cgcacaatga tttacgtcac ccacgatcag gtcgaagcga
    11581 tgacgctggc cgacaaaatc gtggtgctgg acgcaggtcg cgtggcgcag gttgggaaac
    11641 cgctggagct gtaccactat ccggcagacc gttttgtcgc cggatttatc ggttcgccaa
    11701 agatgaactt cctgccggta aaagtgaccg ccaccgcaat cgatcaagtg caggtggagc
    11761 tgccgatgcc aaatcgtcag caagtctggc tgccagttga aagccgtgat gtccaggttg
    11821 gagccaatat gtcgctgggt attcgcccgg aacatctact gccgagtgat atcgctgacg
    11881 tcatccttga gggtgaagtt caggtcgtcg agcaactcgg caacgaaacc caaatccata
    11941 tccagatccc ttccattcgt caaaacctgg tgtaccgcca gaacgacgtg gtgttggtag
    12001 aagaaggtgc cacattcgct atcggcctgc cgccagagcg ttgccatctg ttccgtgagg
    12061 atggcactgc atgtcgtcga ctgcataagg agccgggcgt ttaagcaccc cacaaaacac
    12121 acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat gactcaggag atagaatgat
    12181 gattactctg cgcaaacttc ctctggcggt tgccgtcgca gcgggcgtaa tgtctgctca
    12241 ggcaatggct gttgatttcc acggctatgc acgttccggt attggctgga caggtagcgg
    12301 cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt aaataccgtc ttggcaacga
    12361 atgtgaaact tatgctgaat taaaattggg tcaggaagtg tggaaagagg gcgataagag
    12421 cttctatttc gacactaacg tggcctattc cgtcgcgcaa cagaatgact gggaagctac
    12481 cgatccggcc ttccgtgaag caaacgtgca gggtaaaaac ctgatcgaat ggctgccagg
    12541 ttccaccatc tgggcaggta agcgcttcta ccaacgtcat gacgttcata tgatcgactt
    12601 ctactactgg gatatttctg gtcctggtgc cggtctggaa aacatcgatg ttggcttcgg
    12661 taaactctct ctggcagcaa cccgctcctc tgaagctggt ggttcttcct cttttgccag
    12721 caacaatatt tatgactata ccaacgaaac cgcgaacgac gttttcgatg tgcgtttagc
    12781 gcagatggaa atcaacccgg gcggcacatt agaactgggt gtcgactacg gtcgtgccaa
    12841 cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa gacggctggt tattcactgc
    12901 tgaacatact cagagtgtcc tgaagggctt taacaagttt gttgttcagt acgctactga
    12961 ctcgatgacc tcgcagggta aaggtctgtc gcagggttct ggcgtcgcgt ttgataacga
    13021 aaaatttgcc tacaatatca acaacaacgg tcacatgctg cgtatcctcg accacggtgc
    13081 gatctccatg ggcgacaact gggacatgat gtacgtgggt atgtaccagg atatcaactg
    13141 ggataacgac aacggcacca agtggtggac cgttggtatt cgcccgatgt acaagtggac
    13201 gccaatcatg agcaccgtga tggaaatcgg ctacgacaac gtcgaatccc agcgcaccgg
    13261 cgacaagaac aatcagtaca aaattaccct cgcacaacaa tggcaggctg gcgacagcat
    13321 ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc aagtgggatg agaaatgggg
    13381 ttatgactac aacggcgata gcaaggttaa cccgaactac ggcaaagccg ttcctgctga
    13441 tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag tggaccttcg gtgcccagat
    13501 ggaaatctgg tggtaatagc aaaacctggg ccggataagg cgtttacgcc gcattcggca
    13561 accaacgcct gatgcgacgc ttgcgcgtct tatcaggccg acaacggctg tcaaatgtag
    13621 gccggataag gcgtttacgc cgcatccggc ataaaaacag gttgtcatta tctgaaaggg
    13681 gcgaaagccc ctctgattat cgggtttagc gcgctattgc ctggctaccg ctgagctcca
    13741 gattttgagg tgaaaacaat gaaaatgaat aaaagtctca tcgccctctg tttatcagca
    13801 gggttactgg caagcgcgcc tggaatcagc cttgccgatg ttaactacgt accgcaaaac
    13861 accagcgacg cgccagccat tccatctgct gcgctgcaac aactcacctg gacaccggtc
    13921 gatcaatcta aaacccagac cacccaactg gcgaccggcg gccaacaact gaacgttccc
    13981 ggcatcagtg gtccggttgc tgcgtacagc gtcccggcaa acattggcga actgaccctg
    14041 acgctgacca gcgaagtgaa caaacaaacc agcgtttttg cgccgaacgt gctgattctt
    14101 gatcagaaca tgaccccatc agccttcttc cccagcagtt atttcaccta ccaggaacca
    14161 ggcgtgatga gtgcagatcg gctggaaggc gttatgcgcc tgacaccggc gttggggcag
    14221 caaaaacttt atgttctggt ctttaccacg gaaaaagatc tccagcagac gacccaactg
    14281 ctcgacccgg ctaaagccta tgccaagggc gtcggtaact cgatcccgga tatccccgat
    14341 ccggttgctc gtcataccac cgatggctta ctgaaactga aagtgaaaac gaactccagc
    14401 tccagcgtgt tggtaggacc tttatttggt tcttccgctc cagctccggt tacggtaggt
    14461 aacacggcgg caccagctgt ggctgcaccc gctccggcac cggtgaagaa aagcgagccg
    14521 atgctcaacg acacggaaag ttattttaat accgcgatca aaaacgctgt cgcgaaaggt
    14581 gatgttgata aggcgttaaa actgcttgat gaagctgaac gcctgggatc gacatctgcc
    14641 cgttccacct ttatcagcag tgtaaaaggc aaggggtaat tacgccccac agtgctgatt
    14701 ttgcaacaac tggtgcgtct cctggcgcac ctttttttat gcttccttcc tgggatatga
    14761 gcgatttttt atagtaactc acttcttctt cactaagaat atccattatc tcaatgcctt
    14821 atcagagatt cttttccttt cgccggtagt gtctggacat tcaggctact tttccaggtt
    14881 attttatttc tgttatgcag aggttttatg ataagtcata tcctaaattc tggcggcaat
    14941 aactctttga tgaaacatga tgtggtgcaa ggaaataata tagtagatct tgatttacta
    15001 cgtaatttca atggggtgcc aggtttaaac agagataact ttatttatat cagcaatatt
    15061 ttttcaaata taaaacaacg gaacgaaaaa atcatgcaat aaatttgttt cgtgaagtct
    15121 caatcagtaa tgatactata agtgtaaaat tctacagaaa tgaaaaaaat tgaatgcgct
    15181 tgcaattttc tgatggataa agatgcgcag gggtatatcg acctgtctga tttggattta
    15241 acaagttgtc attttaaagg tgacgttata tcgaaggtgt cttttttatc atcaaatcta
    15301 caacatgtaa cattcgaatg taaagaaatt ggggattgca attttactac tgcaatagtt
    15361 gataatgtca tatttagatg tcgacgttta cacaatgtga tttttatcaa agcgagtggt
    15421 gaatgtgtcg atttcagcaa aaatattctt gatacagttg acttctcgca gagtcaactt
    15481 actcagagta attttcgcga atgtcagatt agaaattcaa acttcgataa ttgttatctt
    15541 tac
//
LOCUS       DAZFDP010000115        15471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFDP010000115 DAZFDP010000000
VERSION     DAZFDP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..66
                     /locus_tag="ACHJ1G_003608"
     CDS             <1..66
                     /locus_tag="ACHJ1G_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765387.1"
                     /translation="RYQAKYVSVIWQKLIPNQADF"
     gene            131..379
                     /locus_tag="ACHJ1G_003609"
     CDS             131..379
                     /locus_tag="ACHJ1G_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4765388.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            357..1487
                     /locus_tag="ACHJ1G_003610"
     CDS             357..1487
                     /locus_tag="ACHJ1G_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4765389.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="ACHJ1G_003611"
     CDS             complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="ACHJ1G_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4765390.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2411..2500
                     /locus_tag="ACHJ1G_003612"
                     /pseudo
     CDS             2411..2500
                     /locus_tag="ACHJ1G_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2528..3271
                     /gene="yciC"
                     /locus_tag="ACHJ1G_003613"
     CDS             2528..3271
                     /gene="yciC"
                     /locus_tag="ACHJ1G_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4765391.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3301..3840
                     /gene="yciB"
                     /locus_tag="ACHJ1G_003614"
     CDS             3301..3840
                     /gene="yciB"
                     /locus_tag="ACHJ1G_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4765392.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3945..4343
                     /gene="yciA"
                     /locus_tag="ACHJ1G_003615"
     CDS             3945..4343
                     /gene="yciA"
                     /locus_tag="ACHJ1G_003615"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4765393.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4383..5102)
                     /gene="tonB"
                     /locus_tag="ACHJ1G_003616"
     CDS             complement(4383..5102)
                     /gene="tonB"
                     /locus_tag="ACHJ1G_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4765394.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5326..5622
                     /gene="yciI"
                     /locus_tag="ACHJ1G_003617"
     CDS             5326..5622
                     /gene="yciI"
                     /locus_tag="ACHJ1G_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4765395.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5921..7174
                     /gene="kch"
                     /locus_tag="ACHJ1G_003618"
     CDS             5921..7174
                     /gene="kch"
                     /locus_tag="ACHJ1G_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4765396.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7229..7402)
                     /gene="yciY"
                     /locus_tag="ACHJ1G_003619"
     CDS             complement(7229..7402)
                     /gene="yciY"
                     /locus_tag="ACHJ1G_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4765397.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7545..9005
                     /gene="cls"
                     /locus_tag="ACHJ1G_003620"
     CDS             7545..9005
                     /gene="cls"
                     /locus_tag="ACHJ1G_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4765398.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9040..9369
                     /gene="yciU"
                     /locus_tag="ACHJ1G_003621"
     CDS             9040..9369
                     /gene="yciU"
                     /locus_tag="ACHJ1G_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4765399.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9422..10426)
                     /gene="oppF"
                     /locus_tag="ACHJ1G_003622"
     CDS             complement(9422..10426)
                     /gene="oppF"
                     /locus_tag="ACHJ1G_003622"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4765400.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10423..11436)
                     /gene="oppD"
                     /locus_tag="ACHJ1G_003623"
     CDS             complement(10423..11436)
                     /gene="oppD"
                     /locus_tag="ACHJ1G_003623"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4765401.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11448..12356)
                     /gene="oppC"
                     /locus_tag="ACHJ1G_003624"
     CDS             complement(11448..12356)
                     /gene="oppC"
                     /locus_tag="ACHJ1G_003624"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4765402.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12371..13291)
                     /gene="oppB"
                     /locus_tag="ACHJ1G_003625"
     CDS             complement(12371..13291)
                     /gene="oppB"
                     /locus_tag="ACHJ1G_003625"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4765403.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13377..15008)
                     /gene="oppA"
                     /locus_tag="ACHJ1G_003626"
     CDS             complement(13377..15008)
                     /gene="oppA"
                     /locus_tag="ACHJ1G_003626"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4765404.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3961 a         3767 c         3654 g         4089 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagttatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc atgattagcg
      121 aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg aattcagtga
      181 accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga tatttcctct
      241 ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg tcggaatggt
      301 aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac tggaagatgg
      361 cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt gacaaaagaa
      421 ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac ggtctcggca
      481 gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt cttgcccggc
      541 agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc ggcggttcat
      601 caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga tatgaaaacg
      661 gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac
      721 gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag
      781 agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg
      841 ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg
      901 caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga
      961 tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga
     1021 tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt
     1081 atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt
     1141 gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat tgcgttctta
     1201 gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta
     1261 agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc
     1321 gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag cgattgttca
     1381 gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa atgaccgata
     1441 tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc
     1501 agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata
     1561 aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta
     1621 gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc acggttaatc
     1681 agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact gagatcggaa
     1741 agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt gtagttaata
     1801 ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca ctgcgccatc
     1861 agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt gccgatttta
     1921 tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc catataagta
     1981 aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta
     2041 ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgcct
     2101 gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc cgccactgtt
     2161 aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacctacatt
     2221 ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca tttattgatc
     2281 tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat caatttttaa
     2341 atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc ccacgcctga
     2401 aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac ctgatgctgc
     2461 cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata aaactcaagg
     2521 agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt tcttccgtaa
     2581 tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag tggtgttagg
     2641 gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg tgcccgttag
     2701 cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc aacaaatttt
     2761 gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca ttctcgccgg
     2821 aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg cgctgcgagc
     2881 tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc tgactaccct
     2941 tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg ccattttact
     3001 ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct cgatgcgtag
     3061 cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac tgagctggtt
     3121 gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa ccccggaaat
     3181 tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc tcatctatct
     3241 gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg gaatcgtaaa
     3301 atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta caagatttat
     3361 gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct tatctatagc
     3421 tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct ggtggtcgtc
     3481 ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa ggttacagtc
     3541 atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa aaagccgcta
     3601 attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc gaagctaaat
     3661 ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat cgcattctgg
     3721 ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct taccttaatc
     3781 ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga taaatcctaa
     3841 ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc aggaaaatca
     3901 tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata
     3961 acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca
     4021 atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg
     4081 cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg actttcttac
     4141 ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga
     4201 catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa ccaatcgggc
     4261 aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct gaaggaaaac
     4321 ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt ttgactttct
     4381 gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac aatcccactg
     4441 cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac
     4501 atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc cggcgtgacg
     4561 tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc
     4621 ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt
     4681 gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa cggtgatgcc
     4741 ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt tttcaccggc
     4801 ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc ttcttttggc
     4861 ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg tggcggctga
     4921 acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat tggctgcgca
     4981 ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc cacaacagca
     5041 ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa atcaagggtc
     5101 attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg caaatagcaa
     5161 tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg gcgtaattga
     5221 ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat cttcgccctt
     5281 tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt gtatgttatt
     5341 tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc ggcacattta
     5401 gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc aatgccagcg
     5461 gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat tgctgaattt
     5521 gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc ggcaggagtc
     5581 tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat tcaatattta
     5641 agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg gtcagtgcat
     5701 tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gaacatattc
     5761 aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt actgccgata
     5821 aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga aaatgtgcac
     5881 gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact gggctacatt
     5941 caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg cgctggccgt
     6001 ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca atttgcttga
     6061 tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa acgccccact
     6121 atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc gggcaaagct
     6181 cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc tgcattttta
     6241 tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt tgctgatact
     6301 gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat ttattagttt
     6361 cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag gttttaatcc
     6421 gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt caaccgtcgg
     6481 ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt cggtcattat
     6541 ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc ttatccgcgg
     6601 gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag atcattttat
     6661 tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc aacgcggaca
     6721 aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg agcaacgctt
     6781 aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat taaagaaagc
     6841 gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag ataacgcgtt
     6901 tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg ccgtcagtga
     6961 tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct caccgcaact
     7021 gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata atgatatgct
     7081 cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg aacaagaaac
     7141 gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag agtaaactta
     7201 ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac aataacgagt
     7261 tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt cggcgcgatt
     7321 gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc cagcgcccca
     7381 cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg gtcattatac
     7441 ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga tgaatccata
     7501 tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca accgtttata
     7561 cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc gtaactttac
     7621 gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg attatttaca
     7681 ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat ttaggcaaac
     7741 gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt aacgacctta
     7801 aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca ttattcaagc
     7861 tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa ctgatgaccg
     7921 agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc cataatattg
     7981 agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct gaatcattaa
     8041 tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc gggagtgtgg
     8101 cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa gtggtcgaag
     8161 ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc caacatcgca
     8221 agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg gtcgatcctc
     8281 gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg cgtatggaag
     8341 gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt gaaaccggaa
     8401 aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag gccagcggtc
     8461 acaccattca cacaattgct tctggccccg gctttccgga agatctcatt caccaggcat
     8521 tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc tactttgtgc
     8581 caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg gatgtcagta
     8641 ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc gcattcttta
     8701 cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta ctgcatacca
     8761 agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt gatatgcgga
     8821 gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt ggtgcagacc
     8881 ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc cgtttatggc
     8941 taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc agtccgttgc
     9001 tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga tctaaacaat
     9061 cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga actggctgcc
     9121 gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga gcgcggcggc
     9181 gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga tttgaaccca
     9241 gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga aatcaacgat
     9301 gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca tatcatctgg
     9361 cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc ccttattgtg
     9421 tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc tccagaacag
     9481 gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa acacaaccgg
     9541 aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt ttgttcttct
     9601 ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa ggatgtagtg
     9661 gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga tacatcacca
     9721 acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa attaatgaca
     9781 atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga attgacacgt
     9841 ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata agagcacgag
     9901 caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg ttaatcaggt
     9961 taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact tcctggcgtg
    10021 acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg gtcatacgcg
    10081 ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga acggcacgcc
    10141 attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga ccgtcggtcg
    10201 ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc gattccccta
    10261 ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg gctttgagcg
    10321 ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc actttaagat
    10381 cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca gttcctccac
    10441 cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg gtgcgctact
    10501 acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg gtaatcgcag
    10561 caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat caagacgcgg
    10621 caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga catcgcgcgc
    10681 attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac agatccccgc
    10741 caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc gcttcagttc
    10801 atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag tgggttcatc
    10861 cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc gctgacgcat
    10921 gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgcgcttccg gcatttttac
    10981 cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt tcttatgcag
    11041 catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg aagtcattgg
    11101 gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat gctctggcaa
    11161 attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt tggcagccaa
    11221 caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac ctacaatacc
    11281 cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga cgtcgccgtc
    11341 cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag cctgttgttg
    11401 tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta acgatctttc
    11461 gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag cgtcaccacg
    11521 aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt cgcgccatca
    11581 ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag gaagctaagg
    11641 aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac caccacaccg
    11701 agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc gccaacttgt
    11761 gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac aatacgagcc
    11821 atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt ttgaccgaaa
    11881 aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat ttccagcaga
    11941 cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc ataaagtgtc
    12001 ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat acgcccgcca
    12061 atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta gtgaccggac
    12121 tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc aaactgggaa
    12181 agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag actggcaacc
    12241 gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg cccttcgacc
    12301 tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa catcatcgcg
    12361 agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca tcgacaatgg
    12421 cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact aaggaatagt
    12481 cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca taaatggttt
    12541 cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa gagagcacgg
    12601 gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt aaccctttcg
    12661 cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca cgggtaatac
    12721 gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc atgaatttaa
    12781 gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca aatatcatga
    12841 ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc agccccatca
    12901 cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca ctaaccccca
    12961 gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga aaactggatg
    13021 caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa tcaccgtgcg
    13081 ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga tgatatttct
    13141 cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa gggcttcccg
    13201 gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc ggaatcgctt
    13261 ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg tgccttctgt
    13321 aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta ttgccattag
    13381 tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt gccggtatag
    13441 ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg aacaatggcc
    13501 gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg cgcctcgtca
    13561 gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata atgcgcggta
    13621 ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt gtagtcagca
    13681 caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag gaacgttttc
    13741 cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa tgaagaggca
    13801 gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat ggtcaacggt
    13861 ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt acgtttttcc
    13921 tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata cggtggagtg
    13981 taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat atcgcggtcc
    14041 atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt ctggttgtta
    14101 atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg gatctctttt
    14161 ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat ttcaccacta
    14221 cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac ctggttaata
    14281 acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg ttcgtttacg
    14341 acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg ggtccatttt
    14401 tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg aacaagtaat
    14461 ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc atcaatagct
    14521 ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat accggcgata
    14581 tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac agaacgttgc
    14641 cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt cgcatcttta
    14701 cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc gacgccaggt
    14761 gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg gctgatatta
    14821 gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga accattgtta
    14881 cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc tgccagcgcg
    14941 acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct cttggtgatg
    15001 ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag catgtgtaat
    15061 cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac tttatcgtta
    15121 cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct tatgtcgatt
    15181 cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt aattgttaat
    15241 tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca aatgcggttc
    15301 ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga ccgtatcaga
    15361 atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa tagagattgg
    15421 ctaagcatgt ctatcttttc tgattacagt tctagtagtg aaatgcacaa t
//
LOCUS       DAZFDP010000116        15297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFDP010000116 DAZFDP010000000
VERSION     DAZFDP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..606
                     /gene="yceJ"
                     /locus_tag="ACHJ1G_003627"
     CDS             40..606
                     /gene="yceJ"
                     /locus_tag="ACHJ1G_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4765405.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            610..1185
                     /gene="yceI"
                     /locus_tag="ACHJ1G_003628"
     CDS             610..1185
                     /gene="yceI"
                     /locus_tag="ACHJ1G_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4765406.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1227..2279)
                     /gene="trhO"
                     /locus_tag="ACHJ1G_003629"
     CDS             complement(1227..2279)
                     /gene="trhO"
                     /locus_tag="ACHJ1G_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4765407.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2504..3424
                     /gene="lpxL"
                     /locus_tag="ACHJ1G_003630"
     CDS             2504..3424
                     /gene="lpxL"
                     /locus_tag="ACHJ1G_003630"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4765408.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3596..4822
                     /gene="mdtG"
                     /locus_tag="ACHJ1G_003631"
     CDS             3596..4822
                     /gene="mdtG"
                     /locus_tag="ACHJ1G_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO4765409.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4905..5279
                     /gene="msyB"
                     /locus_tag="ACHJ1G_003632"
     CDS             4905..5279
                     /gene="msyB"
                     /locus_tag="ACHJ1G_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4765410.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5280..5507)
                     /gene="yceK"
                     /locus_tag="ACHJ1G_003633"
     CDS             complement(5280..5507)
                     /gene="yceK"
                     /locus_tag="ACHJ1G_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4765411.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5680..8223)
                     /gene="mdoH"
                     /locus_tag="ACHJ1G_003634"
     CDS             complement(5680..8223)
                     /gene="mdoH"
                     /locus_tag="ACHJ1G_003634"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4765412.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8216..9751)
                     /gene="mdoG"
                     /locus_tag="ACHJ1G_003635"
     CDS             complement(8216..9751)
                     /gene="mdoG"
                     /locus_tag="ACHJ1G_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4765413.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10145..11302
                     /gene="mdoC"
                     /locus_tag="ACHJ1G_003636"
     CDS             10145..11302
                     /gene="mdoC"
                     /locus_tag="ACHJ1G_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4765414.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11310..12791)
                     /gene="clsC"
                     /locus_tag="ACHJ1G_003637"
     CDS             complement(11310..12791)
                     /gene="clsC"
                     /locus_tag="ACHJ1G_003637"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4765415.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12733..13266)
                     /gene="ymdB"
                     /locus_tag="ACHJ1G_003638"
     CDS             complement(12733..13266)
                     /gene="ymdB"
                     /locus_tag="ACHJ1G_003638"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4765416.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13361..13672)
                     /gene="ymdA"
                     /locus_tag="ACHJ1G_003639"
     CDS             complement(13361..13672)
                     /gene="ymdA"
                     /locus_tag="ACHJ1G_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO4765417.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13793..14125)
                     /gene="csgC"
                     /locus_tag="ACHJ1G_003640"
     CDS             complement(13793..14125)
                     /gene="csgC"
                     /locus_tag="ACHJ1G_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4765418.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14184..14639)
                     /gene="csgA"
                     /locus_tag="ACHJ1G_003641"
     CDS             complement(14184..14639)
                     /gene="csgA"
                     /locus_tag="ACHJ1G_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4765419.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14680..15135)
                     /gene="csgB"
                     /locus_tag="ACHJ1G_003642"
     CDS             complement(14680..15135)
                     /gene="csgB"
                     /locus_tag="ACHJ1G_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4765420.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3789 a         3936 c         3655 g         3917 t
ORIGIN      
        1 ccattttctt tctctcgcaa attcgtattg gatgcactta tgtcattcac aaatacccct
       61 gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat tgtctacggc
      121 atgtttgctt taggtctgtg gatggtcacg ctcagttatt acgatggctg gtatcacaaa
      181 gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt tattcgcgtt
      241 ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc catgactcgt
      301 cttgccgcaa aggctggaca tctcgcattg tacctattgc tgttcgccat cggcatcagc
      361 ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg gtttgacgtc
      421 cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct gcatttttgg
      481 ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat ttatggccct gaagcatcat
      541 ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc tgactatgga
      601 gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat gttctctgcc
      661 ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc ctttgttaac
      721 ttccgcatcc agcatcttgg ctatagctgg ttatatggca cctttaaaga tttcgacggt
      781 acttttacct ttgacgaaaa aaatccggct gccgataaag tgaatgtgac aattaacacc
      841 accagcgtcg atactaatca cgccgaacgc gataaacatc ttcgcagtgc agatttcctc
      901 aataccgcaa aatatccaca ggcaacattc acctccacca gcgtgaagaa agacggtgac
      961 gaactggata ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa
     1021 gcgaaattaa ttggtcaggg cgacgaccca tggggtggta aacgtgcagg cttcgaggcc
     1081 gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag atttaggtcc agcttctcag
     1141 gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagcgtg aaatgttgcc
     1201 ggatggcacg catccggcaa tgatatttat tccgttggat caggaatgcc cagtgttgta
     1261 ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc tgcccgacgg
     1321 cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact acaacaacct
     1381 ttgtattttt ccgcacatac tggacactga ataaacagca ggtggcagcc atcattttta
     1441 cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat aatctcatcc
     1501 gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa acgcaccggc
     1561 aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat atgccagact
     1621 ttgttgaatc cgttatgttt catccaggca ctggcctttt cacaacgaat gccgccggtg
     1681 cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc ttttggcagc
     1741 tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc cacttcatac
     1801 tcatagtggt tacgcatgtc gataaatagt gcatcgggat cgtcaagcat ggcgttcact
     1861 tccgccgctt gcagatactc gccaacattg ctggcatcaa agtgaggatc gtcaataccg
     1921 tcggcaacaa tgcgatcgcg taccttcatg cgcagtaccc agaaagattt cccgtcatca
     1981 tccaacgcga tattcaggcg taaaccctct aaagccgggt cgaaggcata gagctgcgcg
     2041 cgaaatgttt caacattgct cgcaggtacg ctgatttgcg cgttaatgcc ctcatgcgcc
     2101 agatacactc gcccaaaaac attcagcgcg gtaaacagct gatataaagc gtcacgggtc
     2161 gccttaggat cggcgatgtg gaaatacttg taaaacgaaa tggtggttcg cggttcgctc
     2221 tcagccaaca ttttggcttt tagcgcgtcg ttggaaatgc ggttgtgtaa cactggcatg
     2281 gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg catgatatag caattatcga
     2341 taattaacat ccacacattt tacgctacat ttgcgcatta aaaattattt gttatttaca
     2401 agtgcggcaa tttcgcccag tcttcagcca caaattttgg ttgcgggcga aaaaatgcga
     2461 caatacatac aattgcccga ataggttgaa aaacaggatt gatatgacga atctacccaa
     2521 gttctccacc gcactgcttc atccgcgtta ttggttaacc tggttgggta ttggcgtact
     2581 ttggttagtc gtgcaattgc cctacccggt tatctaccgc ctcggttgtg gattaggaaa
     2641 actggcgtta cgttttatga aacgacgcgc aaaaattgtg catcgcaacc tggaactgtg
     2701 cttcccggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt tcgaatccgt
     2761 tggcatgggc ctgatggaaa ccggcatggc gtggttctgg ccggaccgcc gaatcgcccg
     2821 ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa aacgcggcat
     2881 cctgttagtt ggcatccatt ttctgacact ggagctgggt gcgcggcagt ttggtatgca
     2941 ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact ggctacaaac
     3001 ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa aaggcatgat
     3061 taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg gatcatgatt acggcccgcg
     3121 ctcaagcgtt ttcgtcccgt tatttgccgt tgagcaggct gcgaccacga ccggaacctg
     3181 gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc gtaagccaga
     3241 tggcaaaggg tatcaattga ttatgctgcc gccagagtgt tctccgccac tggatgatgc
     3301 cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa tgcatcatga tggcaccaga
     3361 gcagtatatg tggttacacc gtcgctttaa aacacgcccg gaaggcgttc cttcacgcta
     3421 ttaaatctcc cctgccggat gcttcagaat ggcatccggc attaccacag caaatccccc
     3481 tgatttagcg ataaaagctc tctggattgc gccccctgga agtcgagcgc ataattagtg
     3541 tgcttatctt ttcttcttat gttcaccgcg cctggcgcac caacagcgga ttgctatgtc
     3601 accctgtgaa aatgacaccc ctataaactg gaaacgaaac ctgatcgtcg cctggctagg
     3661 ctgttttctt accggtgccg ccttcagtct ggtaatgccc ttcttacccc tctacgttga
     3721 gcagcttggc gttaccggtc actccgccct gaatatgtgg tccggtattg tcttcagcat
     3781 tacattttta ttttcggcca tcgcctcacc gttttggggt ggactcgccg accgtaaagg
     3841 ccgaaaactc atgctattac gctctgccct cggcatgggc atcgtgatgg tgttgatggg
     3901 actggcacaa aatatctggc agtttttgat cctacgggcg cttcttgggt tacttggcgg
     3961 atttgtcccc aacgctaatg ctcttatcgc cacacaagta ccgcgtaata aaagcggctg
     4021 ggcgctgggt acgctctcca caggcggcgt tagtggtgcg ttgctcggcc caatggctgg
     4081 cggcctgctc gccgatagct acggcttacg tccggtattc tttattaccg ccagtgtgct
     4141 catactctgc tttttcgtca ccctgttctg catcagagaa aaattccagc cggtcagcaa
     4201 aaaagagatg ctgcacatgc gggaagtggt gacatcactt aaaaacccga aactggtact
     4261 cagcctgttt gtcaccacgt taatcatcca ggtggcgaca ggctcaattg cccccattct
     4321 gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac gtcgccttta tcagtggcat
     4381 gatcgcctcg gtgccaggcg tggcggctct gctaagcgca ccacgactcg gcaaacttgg
     4441 cgatcgaatc ggacccgaaa agatcctgat tacagcgctg atcttttctg tactgctgtt
     4501 gatcccaatg tcttacgttc agacgccatt gcaacttggg attttacgtt ttttgctcgg
     4561 tgccgccgat ggtgcactac tccccgccgt acagacactg ttggtttaca actcgagcaa
     4621 ccagatcgcc gggcgtatct tcagctataa ccaatcgttt cgtgatattg gcaacgttac
     4681 cggaccattg atgggagcag cgatttcagc gaactacggt ttcagagcgg tatttctcgt
     4741 caccgctggc gtagtgttat tcaacgcagt ctattcatgg aacagtctac gtcgtcgtcg
     4801 aataccccag gtatcgaact gatttttcgc ctttcatact tgcaaaagcg gagaatcagc
     4861 tatccttttc cctgaaacct catcaactca aagggagaat cgtgatgacc atgtacgcaa
     4921 cgcttgaaga agccattgac gctgcacgcg aagaatttct tgcagacaac cccggcatcg
     4981 acgccgaaga tgcgaatgtg caacagttca atgcccaaaa atacgttttg caggacggcg
     5041 acatcatgtg gcaagttgag ttttttgccg acgaagggga agaaggtgaa tgtttaccta
     5101 tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga ctatgatgag atagagatac
     5161 gccaggagtg gcaggaagag aatacattac atgaatggga cgagggggaa tttcagcttg
     5221 agccaccgct ggataccgag gaaggacgcg cagcagctga tgagtgggat gaacgttaat
     5281 cactcatacg ggccatgatg aatgtcgatc ggcagcagta aagtatcaaa aaccagagag
     5341 aatggcagat caaggatcgt gacataacgc caggcggagt cacgcacatc ccattgcaca
     5401 ccgggataat attggttgcc atgcccctgc cccggtatag tgcgactaat aatgctgcca
     5461 cagccactca gcaaggtcac catgatgctc accacaatta aacgcatttg atacctcttt
     5521 cgtaaaaaaa taccggcata acgccggtat ttttattggc tcgttgtttt tgccggatgc
     5581 ggcgtgaacg ccttatccga cccacaaaat cttgcaaaat caataaattg caggaacgat
     5641 gtaggcctga taagcgtagc gcatcaggca actacgtttt tattgcgaag ccgcatccgg
     5701 tttacgcaat gccagtggat tgagctttat cccttcgtaa taactcaccc atgaagaata
     5761 tctctccggg gaattccaga cgcggaaatg cagacgggcc atcgtcaccg gatcgcttag
     5821 cagcaccagg cgacgatcgc gattcagctt ctctggcgtc tcgttcagcg cctgttcaac
     5881 gtggcggtca cgggcgattt ccagcacctt actggcgcgg tgacgcgcgg tcgccattgc
     5941 ggttgccaga gcgttaaatg acgggttaaa cactgcgtgc ataaagccat catcaaggga
     6001 gcgttgacga ttcatctcaa ggaaccgatc ggtatcaacc agcacctgcg gcggcgaata
     6061 ctcttccggg atcaggaaca gtttccagcg tttggtgcgc agaccaacgg tggcacggct
     6121 ggaaatcacc gaaacaaacg gtgacaggat caacgagaag acaatcggtg ccagccagaa
     6181 caggaaacgc agatccaacc acgccatacc aacagcccac actaacccta acagcagctg
     6241 tgagccgtgg cgtttgaacg cttcacccca ggaagtggag tcatcatcac gctgcggtga
     6301 attccacacc acttcccagc caaggaacgc gctgacgacg aagaccgtat ggaacagcat
     6361 gcgtaccgga gccagcagca cggaaaacag cacttccagc agcaacgata atgtaacgcg
     6421 ccagaagccg ccgtattctt tcgttccttt gcaccagata agcaaaatgc tcaataactt
     6481 cggcaggaac aacagcacca tggtcgaagc aaaaagtgca atcgccagct caggacgcca
     6541 ctgcggccac accgggaaca actgccgtgg ttgcaggaag tattgcggtt cggtcaacgc
     6601 atgtactacc tgcaatgcag tagagagcgc gaggaacata aaccacagcg gagcggagag
     6661 ataagacatc acgcccgtca ggaataccgc acggtgaacc gggtgcatac ccttcaccag
     6721 gaacagacgg aagttcatca ggttaccgtg gcaccagcgg cggtcacgtt ttagctcatc
     6781 aagcaagtta ggcggcaatt cttcataaga acccgggaga tcgtaagcaa tccagacccc
     6841 ccaacctgca cggcgcatca acgccgcttc cacgaagtca tgtgacagga ttgaaccggc
     6901 aaaggaacct tcgcccggca gcggagccag tgcgcagtgc tcgataaacg gtttcacgcg
     6961 gataatcgcg ttatgtcccc agtagtgcga ctcgccaagt tgccagaagt gcaaaccggc
     7021 tgtaaacagt ggcccataca cgcgggtcgc gaactgctga cagcgcgcat acagcgtatc
     7081 cataccggac gctttcggcg acgactgaat gatcccggcg ttcgggttgg cttccatcag
     7141 gcgcaccagg ccgcataaac aatcaccggt cattaccgag tcagcatcca gcaccaccat
     7201 gtagctgtac tggctgcccc agcgacggca gaagtcatcg atattaccgc ttttacgctt
     7261 cacgcggcga cggcggcggc gatagaaaat ctgaccttcg ccaccgactt cagcgataag
     7321 ctccatccag gctttttgct ctgcgacgca gatatccggg ttataactgt cactaagaat
     7381 gtagacatca aagtgtttgg cattcccggt ggcttttact gattcccacg ttgcacgcag
     7441 gccagcaaaa acacggttca cgtcttcgtt acagataggc atgatcaacg ccgtgcgatg
     7501 ctccgggttt aatggttcat cgccaactgt tgacgcagat atactgtatt tatcgcgacc
     7561 aataagcagt tgcaggaagc ccattaacgc cgtccagaat ccggcggaca cccaacagaa
     7621 cagtaccgca aagaggatca ggataccggt ttgcagcata taaggcagaa gctgcataaa
     7681 ggaaacccac aaatcctgac caaccatatc cataggatta atcagcgccc aaccctgata
     7741 aggaagaatg gtcttcatat accaggtcgc gacgacagtt tgcgcgagcg tcaggatcaa
     7801 cagaatgtaa cggcggatgg taccgacggt acgccacttt tgctcgctct cctgctcttc
     7861 tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt ccacgcaggc gatcccagaa
     7921 acggcctacc gggttggtac gccacgggtc gggaaacatc gaggagcgtt ttgcttctgg
     7981 catcgccttc agctgatcgc gcccttcgtc gtctttaatt aactgtccat cagcaagtga
     8041 atctggccag gcttgttcca gacgcgcctt taccgagcct tgcggggaat catcctcccg
     8101 cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg gcgcggatat cagtcttcgg
     8161 caatgccgct ttctcgcttg cggcgatggg cattgcgtca atgtactcag ttgtcttatt
     8221 cattggcagg taactggtag ctccaggttt cactcaacgt ctgatcggca ttcaccagcg
     8281 cagcacgcat ttcagtggtt ttcttggcat ctttcacttt cacacgcatc accagacgcc
     8341 agcctttggt aaccgggtta tagcgcaccg tgctttcaac tatctcacca ttatcaccaa
     8401 tgctggtttg cgctgtgacc ggggtatcct ctggcagttt tttcatctct gcgccggtaa
     8461 aatcgaccac aaaggcgata gtaccgtcag gctggcgaat caggttcgac tgcttcacat
     8521 cccccgttga acgacgcgtt tgttgcaccc atgcgttatc tggcgcatgc agtttgtctt
     8581 catcacggct gaaggtgatg gtgtatttaa agttcatctc tttacccggc tccggcagct
     8641 gatccggcgt ccagtaagcg acgatgttat cgttggtttc atcgttggtt ggaatttcca
     8701 ccagctcaac gctgccttta ccccactccc ctttcggagt cacccatgcg cttggacgaa
     8761 gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc acgaccgcgc tgcaacagac
     8821 cgaagccttg cgggttctcc atcgagaagc tgctgaccgc taaatgtttc gggttattca
     8881 acggacgcca gatccattcg ccattaccgg catggataga cagaccgtta gagtcgtgca
     8941 actccggacg atagttattt gcaggcgacg gttggttcgg cccaaacagg aacatactgg
     9001 ttaacggtgc aacccccagt ttgccgactt tatcgcgcag atagattttc gactgcacat
     9061 ccacaaccgt gtcacgccct ggcataacca cgaatttgta agcacctgtc gcgcgcggcg
     9121 agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg ttttggacgc tcgatccaga
     9181 actctttgaa gcgtggaaat tcttcacccg atggcaaggc ggtatcaatt gccaggccgc
     9241 gggcagaaag gccataaacc tgacctgcac caatcacgcg gaaatagctg gccccgagca
     9301 tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg gtaaagcact ttaaaaccgg
     9361 caaaaccaag gtcttttacc gtgtctttgt catgctgaac atcgccgaaa gtgaaataat
     9421 ccgggctgta tttgattcgt ttgactgcgg tggcagtcac ttcatttatt ttgaccgggg
     9481 tatcgaagta cataccctga tggtagaact cgagtttgaa tggggtcttc agattgttcc
     9541 agtacgcttt gtcatgatta aactggatct gctgatagtc cgcgtatttc atatcgcgga
     9601 aaacggaggg caagttgctt ttgggcgcct catagccttt cccggctaag gattgagctt
     9661 gctttgcgac atcatcaata ctgaaagccc agcttgaaga tgtatacagg gttaacatta
     9721 ctgcagcact caaccaacgc attttcatca tttgtagttt atgtttcata attagtaagc
     9781 acttccccct ttgtgtgctt atatcgatcc gatctatttt aatggataac cagccattcc
     9841 gacaacagaa tcccggtatc gttcagcacg ctggctcatg gattaagcac ctgattgcaa
     9901 ccgttttcac tttccgtgct tctcctaaag acagaagcat cgacttcgtg tagggtttgc
     9961 cagcaatttt atttatcaac cgtcttacag acgcttccat tctggttagt ggtagcgatg
    10021 atgctatgca taaaaacctt gaagcctgcg cctggcgcgg tcaggtcggt gtcgctatgg
    10081 caacaagcct aaaggcataa cccgacaggg ataagacgaa aaatccgaga ttagttaacc
    10141 atatatgaat ccagtacccg cgcaacgtga atatttcctc gactccatcc gcgcctggct
    10201 gatgttgtta gggatccctt ttcatatttc tttaatctat tcgagccata catggcatgt
    10261 gaatagcgcc gaaccatcat tgtggctgac cctttttaat gacttcatcc actcgttccg
    10321 catgcaggta tttttcgtta tatccggcta cttttcctac atgctttttt tacgctatcc
    10381 cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt atcccgatgt taacagccat
    10441 ccccctactg acattaccgc aatttattat gctgcaatat gtcaaaggaa aagcggaaag
    10501 ttggcctggg ctgtcattgt atgacaaata taatacgttg gcctgggaat taatatcaca
    10561 cctgtggttt ttactggtgt tagtagtcat gacgacgctg tgcgtatgga tatttaagcg
    10621 catcagaaat aatttagaaa attctgataa aacgaataaa aaattctcga tggtaaaact
    10681 atcggtgatt tttttatgcc tcggcatcgg ttatgcggta ataagaagaa cgatttttat
    10741 tgtgtatccg cccattctga gtaatggcat gttcaatttt attgtcatgc aaacgctgtt
    10801 ttatttgccg ttctttatcc tcggcgcact ggctttcatt ttccctcatc ttaaagcctt
    10861 gtttaccacg ccgtctcgtg gctgtaccct tgcagcagca ttggcgtttg tcgcttattt
    10921 actcaaccag cgctatggca gtggcgatgc ctggatgtac gaaaccgagt cggtgatcac
    10981 catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc tttggccacc gtttgcttaa
    11041 cttccagtca gcgcgggtga cttattttgt taacgcatcg ctgtttatct atctggttca
    11101 ccacccgtta acgctgtttt tcggcgcata cattacaccg cacatcacct ccaactggct
    11161 tggttttctc tgtggcctga tatttgtagt agggattgcg ataattctgt atgaaattca
    11221 tttgcgcatc ccgttactga agtttttgtt ctctggtaaa ccggttgtta agcgtgagaa
    11281 cgataaagca ccagcccgtt aagccacatt tacaataacc attccacggg caatatcgac
    11341 gccagtctga ccataacccg cttccagaaa ctggtggcgg gttctttttt gagaataatc
    11401 tctttctttg catgacgatc aacccagttg atccgtcccc acctgtccag acggagctgc
    11461 caggccgcat catactggct ctgaataaag cgtttatcaa ttaactgtgc cagcgtttcg
    11521 ctctctatca cgaagcccat ttcagtattg agcaatgttg aacgcggatc gaaattgaaa
    11581 gagccgataa acaccgtttt accatcgatg ctaaaggttt tagcatgcag gctggctccg
    11641 gaattaccgg ttatgccgcg atcgtgtaac gtactacttt gttcacgcgt cggcttgagt
    11701 tcatataatt ccacgccata gcggagcaat tttttgcgcc agcgcgcgta tccggcatgg
    11761 acgacagcaa catcgttagc ggcaagagaa ttggttagga tcgcaatctt tacccctttt
    11821 ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg gtacaaaata ggaagagata
    11881 atatcgatgc gttcactggg tgagcccatg atatcgaaca ggcgctgcgg taacagtgaa
    11941 tgccgttttg ccttgccctc ccctttcgcc ggatcatcac ttaataaacg tgttttcgcc
    12001 cagataagcg gcaacgttcc atcaaccaga tgatttataa acggactgga ttccattttg
    12061 cgtaagtaac gatgcgtcat agcatcgtta tgccaggagg cgggtaactc gatgcgatcc
    12121 gccatttcac cttccgggac atccagcact tgctgtaagg gtgaaaccga tttgcaatac
    12181 cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg gccctattgc cataacatct
    12241 aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg catcaccaat atttcgcccc
    12301 cctaccagcg tcaccacgcc atcgacagtg aaacttttat tgtgcatacg gcgattgaga
    12361 cgggaaaagt cggtgatata accaagcgga cgcagcaagc gaaacgagaa aggattaaaa
    12421 agccggactt caatgcgggg atggctgtca agcaagcgta aaatgtcgtc aagtccgggc
    12481 gtattgttgt cgtccagcaa caaacggacg cgaacgccac gctttgctgc ggctaacagg
    12541 gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga tgtaatactg aacatccagc
    12601 gtatgctctg ccatttcggc cagacgatac cgggcggcaa acgcatccag acttttctcc
    12661 agagggaaaa gaccacactg accgggatgt tgcgaacaca gtggcagcac cgcgctcgcc
    12721 agccggggca aatcattcat ctccttgttg ggtaaggagt ctttcgtaga ggtgggcgtt
    12781 ttcttcatca taacagacaa agtatacctg ttcgggtaaa gcgtgacggg taataaattc
    12841 tgaaacggtt ttaactgcga tttcagccgc tgccgcacga gggtaaccat aaaccccagt
    12901 actgattgca ggaaaagcca ctgacgtata gctgtttgcc gccaccagtc gtaggctatt
    12961 gagataggca tcctgcaaaa gctggtcttc gttttgttca ccaccacgcc agactggccc
    13021 gacggtgtgc actacggctt tagcgggaag agcgcctgca agcgtaataa cggcatgccc
    13081 cgtagggcaa tcgccctgct gttgcctgac ttttaaacag gcatccagca gggccggacc
    13141 cgctgcgcga tgaatggccc catcgacgcc gccgcctccc attaatgacg gattagccgc
    13201 attcacaatc acatcaacgg ccagtttggt aatatcaccc tgcacaacat gaatacgcgt
    13261 tttcataacg cctccttaca ccttcttgtt tcttaagtgt acagcaggct ttttggttaa
    13321 atcgcatttt tctgctcagc aacgtgcgtt gttcagaaac ttagttatat tcgatgttca
    13381 ttacagccag ctttttctgt ttatcaagat actgcacttt tactgaggct atttgcgcgt
    13441 ttttaacatt accgctcatt aacgctttgc tggaataggt tttgcccgga acagaaccat
    13501 tctgtgggca gttcatttct acgggtgatg actgggtgct gacgtcacat gccggttcca
    13561 caatttggcc ataaaaatgg atcacccccc cttgcgcggt gcttcctgcg atggcaataa
    13621 aaggaaaaag caaactgccg agcataagag agtttaaaaa aggtctgaac atttattcta
    13681 tttcctcaat gattagtcat ccttgagggt tgtgttatcc atactttcag gttggctgat
    13741 tcgcagcaga ccattctctc cagattcatc ttatgctcga tatttcaaca aattaagact
    13801 tttctgaaga gggcggccat tgttgtgata aatgaagtga ctgtccatca gaaacagtaa
    13861 caactatttt cacccgatct tccggggaaa tatttaaact caacttcgtc aaagcaatag
    13921 gttgattagc aggcaatgag agggtctttt cttgcttcgt ctgactttgc cctgaactgc
    13981 cttcgcgcag ggacaatatt tgtactctgc acagacaaga ttgagtaaga gtgacttcag
    14041 gaataatggt atacacatcc ccttgctggg tcgtattaaa ggttatctgg ctggaaagtg
    14101 ccgcaaggag taataacgca ttcatattct tctcccgaaa aaaaacaggg cttgcgccct
    14161 gtttctgtaa tacaaatgat gtattagtac tgatgagcgg tcgcgttgtt accaaagcca
    14221 acctgagtca cgttgacgga ggagttagat gcagtctggt caactgcagc accgttgcca
    14281 ccaccgaact gtttaacagt catttcagaa tttttgccgt tccactgatc aagagtggcg
    14341 ctgttaccga agccacgttg ggtcagatcg attgagctgt catctgagcc ctgacctaca
    14401 tctgcaccat taccaccgcc atgctgggta atagtcaagt cagagttacg ggcatcagtt
    14461 tgcagagcaa gtgcagagtt accgccaccg tactggtaaa tgttcagctc tgaatttggg
    14521 ccgctattat taccgccacc accgtggtta ccgccgccgc cgtactgagg aacaacacct
    14581 gccagagcgc taccggagaa tacgattgct gcaattgctg ctacttttaa aagtttcatg
    14641 taaaaccccc atcggattga tttaaaagtc gaatggaaat taacgttgtg tcacgcgaat
    14701 agccatttgc gactgtctct gcactacaat tgccgttttt tgagtaccat actgtgtaat
    14761 atttgcttta ttaccagaac ctttctggat aatcatcgca gtattaccat aagcaccttg
    14821 cgaaatactg gcatcgttgg cactgcccgc ctgatcaata tatgcaaggt tataatctcc
    14881 tgtctggtca atctttgccc ggttgctact accttcttgc gcaacaaccg ccaaaagttt
    14941 tgagcctccc tgccgtaact gagcactatt attcgtccca gcttgaccaa ttatggctgc
    15001 ctgattaaat gaagacttac tcaattcatt taccgcgaag ttatattctg aattagctaa
    15061 atcataacct gctgcggctg caatcccagg cgcacccagt attgttaaca tcataaataa
    15121 caatttgttt ttcatgttgt caccctggac ctggtcgtac atttaagaaa ttaaatcatt
    15181 tcaacttggt tgttaacgca acctgtattt tgttaacgct gcgttacgat ggaaagtatg
    15241 tctgcggaaa tatttttaat aactcacccg cgcgttgtat ttttcttttt ttgttca
//
LOCUS       DAZFDP010000117        15281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFDP010000117 DAZFDP010000000
VERSION     DAZFDP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..1044)
                     /gene="gsiD"
                     /locus_tag="ACHJ1G_003643"
     CDS             complement(133..1044)
                     /gene="gsiD"
                     /locus_tag="ACHJ1G_003643"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4765421.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1047..1967)
                     /gene="gsiC"
                     /locus_tag="ACHJ1G_003644"
     CDS             complement(1047..1967)
                     /gene="gsiC"
                     /locus_tag="ACHJ1G_003644"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4765422.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1985..3523)
                     /gene="gsiB"
                     /locus_tag="ACHJ1G_003645"
     CDS             complement(1985..3523)
                     /gene="gsiB"
                     /locus_tag="ACHJ1G_003645"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4765423.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3543..5414)
                     /gene="gsiA"
                     /locus_tag="ACHJ1G_003646"
     CDS             complement(3543..5414)
                     /gene="gsiA"
                     /locus_tag="ACHJ1G_003646"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4765424.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5401..6366)
                     /gene="iaaA"
                     /locus_tag="ACHJ1G_003647"
     CDS             complement(5401..6366)
                     /gene="iaaA"
                     /locus_tag="ACHJ1G_003647"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4765425.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6570..7805
                     /gene="moeA"
                     /locus_tag="ACHJ1G_003648"
     CDS             6570..7805
                     /gene="moeA"
                     /locus_tag="ACHJ1G_003648"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4765426.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7805..8554
                     /gene="moeB"
                     /locus_tag="ACHJ1G_003649"
     CDS             7805..8554
                     /gene="moeB"
                     /locus_tag="ACHJ1G_003649"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4765427.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8630..9292)
                     /gene="fsa"
                     /locus_tag="ACHJ1G_003650"
     CDS             complement(8630..9292)
                     /gene="fsa"
                     /locus_tag="ACHJ1G_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4765428.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGMAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9423..10322
                     /gene="ybiY"
                     /locus_tag="ACHJ1G_003651"
     CDS             9423..10322
                     /gene="ybiY"
                     /locus_tag="ACHJ1G_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4765429.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10328..12760
                     /gene="ybiW"
                     /locus_tag="ACHJ1G_003652"
     CDS             10328..12760
                     /gene="ybiW"
                     /locus_tag="ACHJ1G_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO4765430.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12906..13721
                     /gene="ybiV"
                     /locus_tag="ACHJ1G_003653"
     CDS             12906..13721
                     /gene="ybiV"
                     /locus_tag="ACHJ1G_003653"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4765431.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13873..15138
                     /gene="ybiU"
                     /locus_tag="ACHJ1G_003654"
     CDS             13873..15138
                     /gene="ybiU"
                     /locus_tag="ACHJ1G_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4765432.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3687 a         4229 c         3905 g         3460 t
ORIGIN      
        1 aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa aatcagcaga
       61 aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct ttcaagcaat
      121 attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac catcgcccaa
      181 caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg
      241 cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg gtgtcggcgg
      301 ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg tgccaatgcg
      361 catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac gcaataaaat
      421 ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct gtttcaacac
      481 cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat
      541 aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc
      601 cgggaaggca aacagcacat cgcaaatgcg catgatcaac cgatcccacc agccttcata
      661 atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca cggcaaacac
      721 gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag
      781 tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt cataatcaaa
      841 ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca caatcaataa
      901 aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc gccagaattc
      961 atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta acaccgcctg
     1021 acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt aatggcggcg
     1081 taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag cagaatttcc
     1141 gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg tccaagtccg
     1201 ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc aaactgtaag
     1261 cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag gacaacccag
     1321 gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa aacatcgaca
     1381 aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag cgtcagggag
     1441 ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc cagttcaacg
     1501 gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat ccccgacacc
     1561 gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc gataattccc
     1621 gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca cagcgttggc
     1681 ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga caggccaaaa
     1741 tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg ctgatccaac
     1801 cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgggccc ggcaatcaat
     1861 cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga gacgataaac
     1921 agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc actccctgcg
     1981 ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg atccaaaaac
     2041 cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg atccacggcg
     2101 actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc gccggatcat
     2161 tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa aatgcggtat
     2221 taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc cagtccgctt
     2281 cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct ttttgccctt
     2341 taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg gctttaatcc
     2401 cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat
     2461 gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc
     2521 gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg atacttggcg
     2581 gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc agcgccggac
     2641 ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca
     2701 cgttcatact gatataacgc tgcataattg acggactggc catcaactcg atatttttgt
     2761 ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg
     2821 tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt atgctgtcca
     2881 gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca aaatcggtct
     2941 gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca atctccttgc
     3001 catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca agaatattaa
     3061 taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg
     3121 ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc
     3181 ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg aatttaattc
     3241 cttcccgcaa tttcacgatg taagtaatgc cgtcatcgga aacggtataa ctctccgcca
     3301 gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg
     3361 ctacggcctg agataacgtg tcatttgcgt catacggatc gagcgtggtg aaattcgatc
     3421 ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa
     3481 tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa
     3541 tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg
     3601 acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga
     3661 tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc
     3721 gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg ccgtggacca
     3781 atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg ctctaccacg
     3841 gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac
     3901 aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc gtcggcaatg
     3961 atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg
     4021 gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac
     4081 cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg cagcggttcg
     4141 ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata
     4201 aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc gattctctga
     4261 ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta
     4321 gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca
     4381 aaactaactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc
     4441 aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc caccaccgtt
     4501 ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa cgggaaacgt
     4561 cggggataat ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg
     4621 gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg
     4681 ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg
     4741 ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc
     4801 gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg
     4861 catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgga
     4921 taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat ccgcttcgcc
     4981 tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt
     5041 tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat catcgccata
     5101 tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc aatcacttcg
     5161 cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc ctgttccaac
     5221 aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc aacaattgcc
     5281 agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat tttctgctgg
     5341 tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc atcaagttca
     5401 tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc
     5461 tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattcccttc
     5521 atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac
     5581 ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata
     5641 tgccgccagc gcgcggatga agacttcgcc cgtgccggta caagaaaccg ccacactggc
     5701 gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt
     5761 attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat ccaacgccac
     5821 ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac
     5881 ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat
     5941 ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc cttcgccaat
     6001 catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag
     6061 atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc
     6121 cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca
     6181 ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc ccgcttccaa
     6241 cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta attcctgttg
     6301 cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt
     6361 gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata tcagaatttt
     6421 tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt aaaggcagga
     6481 gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat aataggcaaa
     6541 ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg
     6601 acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag gaaacgctgc
     6661 cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg cttgatgttc
     6721 cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat attgcctcca
     6781 ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat ggtgaatggc
     6841 ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg
     6901 tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc
     6961 gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc
     7021 cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg
     7081 aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt
     7141 tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc gccgtacacc
     7201 tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc gacgatcccc
     7261 atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg
     7321 gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga
     7381 tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt aaactcagca
     7441 atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac
     7501 tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc
     7561 agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg
     7621 gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga catcagggtt
     7681 cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa cgcgatcgcg
     7741 gcaatgtgga agtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc
     7801 tgtaatgacg gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg
     7861 cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct
     7921 cggtggactc ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac
     7981 gctgctcgac ttcgactcgg tttcgctctc gaatctgcaa cgccagacgc tgcacagtga
     8041 tgctacggtc gggcaaccga aggtagaatc cgcccgtgac gccatgacgc ggatcaaccc
     8101 acatatcgcg attacgccag tcaatgcact gcttgatgac gctgaacttg ctgcgatgat
     8161 tgcaaaacac gatctggtac tcgactgtac ggataacgtt gcggtgcgta atcaactgaa
     8221 cgctggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg
     8281 gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt
     8341 gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt
     8401 aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc
     8461 cagcgggaaa atcgtcatgt acgatgcaat gacctgtcag tttcgcgaaa tgaaactgat
     8521 gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc agataagacg
     8581 cggcactccg ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg
     8641 ttctgccaaa cgctcccagc cagtcctgtt caaacttcgc cacagcggct tccaccgccg
     8701 gatagctaat catctgttgt gccacatcca gtggcagcgt aattgattca catcctgcca
     8761 gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc tgccagcact ttcgcctgcg
     8821 gcgcatgcat tttcaataac tggtgtaagt cggtcacagt ctgaatgccg ctaccgccct
     8881 gagcatcaat acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagca
     8941 gcccttgtgc tgcgccatat accgcggttc ccagcgttgg aatcccttcc gcttttaaca
     9001 tcttaatagc tgccatcccc tcggcggtca ccggaacttt caccacgata tccgcaataa
     9061 tagaacgcaa tttacgtgcg tcattcacca tcccttcagc agtggtagcc attacctggg
     9121 caaacagacg cccctgaccg cccatcgctt catgaagttg cggaagcaca acatccagcg
     9181 gtttgttacc cgcggcgata atgcttgggt tagtggtcac acccgccagc ggaaaaatac
     9241 gtgacagcgc cttcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc
     9301 tcaaaatgta ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac
     9361 gcacatcaat ctaactttca ttcgaaagca ttttaatgtt tgaacgaaac agagaggcaa
     9421 ctatgatttt caatattcag cgctactcga cccatgatgg ccccggtatc cgcacggtcg
     9481 tatttcttaa aggctgttcg ctgggctgcc gctggtgtca gaacccggaa agccgcgccc
     9541 gcacgcagga tctgctgtat gacgcacgac tgtgtctgga aggctgcgag ctgtgcgcta
     9601 aggccgcgcc ggaagtgatt gagcgcgcgc tgaatggttt gcttattcat cgggaaaagt
     9661 taaccccgga gcatctgacg gcgttaaccg actgctgtcc gacacaggca ttaaccgtgt
     9721 gtggtgaagt gaaaagcgtt gaggagatca tgacgaccgt tctgcgcgat aaaccgtttt
     9781 atgatcgcag cggcggcggt ttaacgcttt cgggtggtga gccctttatg cagccggaaa
     9841 tggcgatggc gctactgcaa gccagccacg aggcaggcat tcatactgcg gtagaaacct
     9901 gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc ctatatcgat ctgtttcttg
     9961 ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca
    10021 gagtgctgga taacctgaaa aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc
    10081 cgctgattca gggctttaat gccgacgaaa cctctgtaaa agccattacc gattttgccg
    10141 ccgacgagct gcacgttggc gaaattcatt ttctgcccta ccacacgctg ggcatcaaca
    10201 aatatcactt acttaatctg ccctatgacg ccccggaaaa accgcttgat gcgccagaac
    10261 tgctcgactt tgcccagcag tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat
    10321 aacaaccatg accacactga aactggacac gctcagcgac cgcattaaag cgcacaaaaa
    10381 tgcgctggtg catattgtga aaccgccagt ctgtaccgag cgcgcgcagc actataccga
    10441 gatgtatcaa caacatctcg ataagccgat cccggtacgt cgcgcgctgg cactggcgca
    10501 tcacctggcg aatcgcacca tctggatcaa acacgatgag ttgatcattg gcaaccaggc
    10561 aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact gtctcgtgga tcgaaaaaga
    10621 gattgatgat ctggcagatc gtcccggtgc tggctttgcg gtgagcgaag agaacaaacg
    10681 cgttctgcat gaagtgtgcc cgtggtggcg cggtcagacc gtacaggatc gctgctacgg
    10741 catgtttacc gatgagcaaa aaggtctgct ggcgaccgga atcattaaag cggaaggcaa
    10801 tatgacctcc ggcgatgcgc acctggcggt gaatttcccg ctgctgctgg aaaaaggtct
    10861 tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc atcaatctga cggtgctgga
    10921 agatttacat ggcgaacaat tcctgaaagc gattgatatc gtgctggtgg cagtcagtga
    10981 acacattgaa cgtttcgctg ccctggcgcg tgaaatggct gctaccgaaa ctcgcgaaag
    11041 ccgtcgcgat gaactgctga cgatggcaga aaactgcgat cttatcgccc accagccgcc
    11101 gcagactttc tggcaggcgc tgcaactgtg ttacttcatc caattgattt tgcagatcga
    11161 atctaacggt cactcggtat cgtttggtcg tatggaccag tatctctacc cgtactatcg
    11221 ccgcgacgtt gaactcaacc agacgctgga tcgcgaacac gccatcgaga tgctgcatag
    11281 ctgctggctg aaactgctgg aagtgaacaa gatccgctcc ggctcacact caaaagcctc
    11341 tgcgggaagt ccgctgtatc agaacgttac cattggcgga caaaatctgg ttgatggtca
    11401 accaatggat gcagtgaatc ccctctctta cgcgatcctc gaatcctgcg gtcgcctgcg
    11461 ttccactcag cctaacctca gcgtgcgtta ccatgcagga atgagtaatg atttcctcga
    11521 cgcctgcgta caggtgatcc gttgcggctt cgggatgccg gcgttcaaca acgacgaaat
    11581 cgtgatcccg gaatttatta aactcggtat tgaaccgcag gacgcttacg actacgcagc
    11641 gattggttgt atcgaaaccg ccgtcggtgg caaatggggc tatcgctgta ccggcatgag
    11701 ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa ggcgggcgtg atgccaccag
    11761 cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca ggcaacttta acaatttcga
    11821 tgaagtaatg gacgcgtggg atacgcaaat ccgttattac acgcgtaaat caatcgaaat
    11881 cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac gatattctct gctcggcgct
    11941 ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa ggcggcgcga aatatgactg
    12001 ggtttctggc ctgcaggttg gcattgccaa cctcggcaac agcctggcgg cagtgaagaa
    12061 actggtattt gaacagggcg cgattggtca gcaacagctg gctgccgcac tggcggatga
    12121 cttcgatggc ctgactcatg agcaactgcg tcagcggctg attaacggtg cgccgaagta
    12181 cggcaacgac gatgatactg tcgatacgct gctggctcgc gcttatcaga cctatatcga
    12241 cgaactgaaa cagtaccata atccgcgcta cggtcgtggt ccggttggcg gcaactatta
    12301 cgcgggtacg tcatctatct ccgctaacgt accgtttggc gcgcaaacta tggcaacgcc
    12361 ggacgggcgt aaagcccaca ccccgctggc agaaggcgct agtccggcct ctggtactga
    12421 ccatctcggc cctactgcgg tcattggctc ggtgggtaaa ctgcctacgg cagcgattct
    12481 cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg ctggagaacg aatctgacaa
    12541 gcagaagctg atgatcctgc tgcgcacctt cttcgaggtg cataaaggct ggcatattca
    12601 gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa aaacatccgg atcagtaccg
    12661 cgatctggta gtgcgtgtcg cgggttattc cgccttcttc accgcgctct ctccagacgc
    12721 tcaggacgat atcatcgctc gtactgaaca tatgctgtaa tctcttcggc ccggctgcgg
    12781 cgtcgccggg ctaaatcatt cacatcatca atttcatcct tactttcatt cgaaatataa
    12841 tttgtgctct gcgtcacatt gttattacac tgttacggtt tcattttcga gccaggagca
    12901 cacctatgag cgtaaaagtt atcgtcacag acatggacgg tacttttctt aacgacgcca
    12961 aaacgtacaa ccaaccacgt tttatggcgc aatatcagga actgaaaaag cgcggcattg
    13021 agttcgttgt cgccagcggt aatcagtatt accagcttat ttcgttcttt cctgagctaa
    13081 aggatgagat ctcttttgtc gcggaaaacg gcgcactggt ttacgaacat ggcaagcaac
    13141 tgttccacgg cgaactgacc cgacatgaat cgcggattgt tattggcgag ttgctaaaag
    13201 ataagcaact caattttgtc gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc
    13261 ccgaagcatt tgtcgcactg atggcaaaac actaccatcg cctgaaagct gtaaaagatt
    13321 atcaggagat tgacgacgta ctgttcaagt tttcgctcaa cctgccagat gaacaaatcc
    13381 cgttagtgat cgacaaactg cacatagcgc tcgatggcat tatgaaaccc gtcaccagtg
    13441 gttttggctt tatcgacctg attattcccg gtctacataa agcaaacggt atttcgcggt
    13501 tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc gattggcgac agcggtaacg
    13561 atgcggagat gctgaaaatg gcgcgttatt cctttgcgat gggcaatgct gcggaaaaca
    13621 ttaaacaaat cgcccgttac gctaccgatg ataataatca tgaaggcgcg ctgaatgtga
    13681 ttcaggcggt gctggataac acatcccctt ttaacagctg acctctctac cggagccgtg
    13741 cgctccggtt ttcccttctt tttacatcct gtacatcaca tcaaatcatt aaacttgcat
    13801 taacttattt ataacttata ttgtcacatg caaatcagaa tggtttcgaa tgaaagttaa
    13861 tgagaggtgg ttatggcttc tacttttacc agcgacacat tgcctgccga tcacaaagca
    13921 gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg gcgacgtcca gcagatcttt
    13981 aatcagctaa gcgatgacat tgccacgcga gtggctgaaa tcaacgcact caaagcacag
    14041 ggcgatgccg tctggccggt gctgtcttat gccgatatca aagcaggtca tgttactgca
    14101 gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga taaaaggcca tttcccccgc
    14161 gaacaagcgc taggctggga tcagtcgatg ctggactatc tggaccgcaa ccgctttgac
    14221 gaggtctaca aaggccccgg cgataatttc ttcgggacgc tcagcgcttc acgtcccgag
    14281 atttacccca tctactggtc gcaggcgcaa atgcaggccc gccagagtga agaaatggcg
    14341 aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa gtgatggaaa gcaatggttt
    14401 aacccggatg tgagcgtcat ctaccctgac cgtatccgcc gccgtccgcc cggaacgacc
    14461 tccaaaggtc ttggagcgca taccgactcc ggggcgctgg aacgctggct gcttccagcg
    14521 tatcagcgcg ttttcgccaa cgtctttaat ggcaatctgg cgcaatatga tccctggcat
    14581 gcggcacatc gtacggaagt tgaagagtac acggtggaca acaccaccaa atgttccgtg
    14641 tttcggacat tccagggctg gacagcgctc tctgatatgc tgcctggtca ggggctgctg
    14701 cacgttgtgc ccattcctga agccatggcg tacgtactgt tacgtccgct gcttgatgat
    14761 gtgccggagg atgaactgtg cggcgtagcg cccggaagag tgttgccggt atcagagcaa
    14821 tggcatccac tgttaattga ggcgttaacc agcattccaa aactcgaggc cggagactcc
    14881 gtctggtggc actgcgacgt catccattcc gttgcccccg ttgaaaatca acagggttgg
    14941 ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga aaaatcttgc ctacgcgcac
    15001 aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg acttcccgcg cgaggactat
    15061 gaaacaaact gggaaggacg ctttacgctt gccgacctca acattcacgg taagcgagcg
    15121 ttgggcatgg atgtttgaga gttaatggaa gtgaattgat gtgggattaa gaaaatcgat
    15181 tcgacgttac tcagattgct gacaagtgcg ctttcttaat gcctgatgcg ctacgcttat
    15241 ctggcctaca aagtcctgca gattcaatat attacaggta c
//
LOCUS       DAZFDP010000118        15244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFDP010000118 DAZFDP010000000
VERSION     DAZFDP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            247..582
                     /locus_tag="ACHJ1G_003655"
     CDS             247..582
                     /locus_tag="ACHJ1G_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4765433.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            742..1185
                     /gene="yjdN"
                     /locus_tag="ACHJ1G_003656"
     CDS             742..1185
                     /gene="yjdN"
                     /locus_tag="ACHJ1G_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4765434.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1318..2106
                     /gene="phnC"
                     /locus_tag="ACHJ1G_003657"
     CDS             1318..2106
                     /gene="phnC"
                     /locus_tag="ACHJ1G_003657"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4765435.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2131..3147
                     /gene="phnD"
                     /locus_tag="ACHJ1G_003658"
     CDS             2131..3147
                     /gene="phnD"
                     /locus_tag="ACHJ1G_003658"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4765436.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3253..4032
                     /gene="phnE"
                     /locus_tag="ACHJ1G_003659"
     CDS             3253..4032
                     /gene="phnE"
                     /locus_tag="ACHJ1G_003659"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4765437.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4053..4778
                     /gene="phnF"
                     /locus_tag="ACHJ1G_003660"
     CDS             4053..4778
                     /gene="phnF"
                     /locus_tag="ACHJ1G_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4765438.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4779..5231
                     /gene="phnG"
                     /locus_tag="ACHJ1G_003661"
     CDS             4779..5231
                     /gene="phnG"
                     /locus_tag="ACHJ1G_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4765439.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5228..5812
                     /gene="phnH"
                     /locus_tag="ACHJ1G_003662"
     CDS             5228..5812
                     /gene="phnH"
                     /locus_tag="ACHJ1G_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4765440.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5812..6876
                     /gene="phnI"
                     /locus_tag="ACHJ1G_003663"
     CDS             5812..6876
                     /gene="phnI"
                     /locus_tag="ACHJ1G_003663"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4765441.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6869..7714
                     /gene="phnJ"
                     /locus_tag="ACHJ1G_003664"
     CDS             6869..7714
                     /gene="phnJ"
                     /locus_tag="ACHJ1G_003664"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4765442.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7711..8469
                     /gene="phnK"
                     /locus_tag="ACHJ1G_003665"
     CDS             7711..8469
                     /gene="phnK"
                     /locus_tag="ACHJ1G_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4765443.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8580..9260
                     /gene="phnL"
                     /locus_tag="ACHJ1G_003666"
     CDS             8580..9260
                     /gene="phnL"
                     /locus_tag="ACHJ1G_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4765444.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9257..10393
                     /gene="phnM"
                     /locus_tag="ACHJ1G_003667"
     CDS             9257..10393
                     /gene="phnM"
                     /locus_tag="ACHJ1G_003667"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4765445.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10393..10950
                     /gene="phnN"
                     /locus_tag="ACHJ1G_003668"
     CDS             10393..10950
                     /gene="phnN"
                     /locus_tag="ACHJ1G_003668"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4765446.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10937..11371
                     /gene="phnO"
                     /locus_tag="ACHJ1G_003669"
     CDS             10937..11371
                     /gene="phnO"
                     /locus_tag="ACHJ1G_003669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO4765447.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11373..12131
                     /gene="phnP"
                     /locus_tag="ACHJ1G_003670"
     CDS             11373..12131
                     /gene="phnP"
                     /locus_tag="ACHJ1G_003670"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4765448.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12278..12607)
                     /gene="yjdP"
                     /locus_tag="ACHJ1G_003671"
     CDS             complement(12278..12607)
                     /gene="yjdP"
                     /locus_tag="ACHJ1G_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4765449.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12676..12993)
                     /locus_tag="ACHJ1G_003672"
     CDS             complement(12676..12993)
                     /locus_tag="ACHJ1G_003672"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO4765450.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13246..15231
                     /gene="yjcS"
                     /locus_tag="ACHJ1G_003673"
     CDS             13246..15231
                     /gene="yjcS"
                     /locus_tag="ACHJ1G_003673"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4765451.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3471 a         4502 c         4186 g         3085 t
ORIGIN      
        1 atcagaaaca taaaaatatc atgtgaatta aaaaaaagaa caagtagagc attaacatta
       61 tcttaaataa taaatggagg caaaaagatt attttctgtt tgcgtttcct ttcaaatgaa
      121 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
      181 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
      241 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
      301 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
      361 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
      421 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
      481 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
      541 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
      601 gccggataag gcgctcgcgc cgcatccggc aacgatgccg actgcctgat gcgacgctga
      661 ccgcgtctta tcaggcttcc ctctcatctg tataaatttc gaactacact taactggctt
      721 ctcttaactg aggtcaccat catgtcgtta agtccctacc tctcttttgc cggtaactgt
      781 gccgacgcaa ttgcttatta tcaacgtacg ttgggcgctg aactgctcta taaaatcagc
      841 ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga actgcccttc cggaatgcaa
      901 tttcccgata ccgccatcgc ccacgccaac gtgcgcattg ccggaagcga catcatgatg
      961 agcgatgcta taccgtcagg aaaagccagc tactccggct ttacgctggt gctcgattcg
     1021 caacaggtcg aagaaggaaa acgctggttt gacaatcttg ccgctaacgg aaaaatcgaa
     1081 atggcctggc aggaaacttt ctgggcgcat ggctttggca aagtcaccga taaatttggc
     1141 gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataacccgc cgggaggccc
     1201 gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca cttttaatta
     1261 accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg ctgcatcatg
     1321 caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca ggcgctgcat
     1381 gcggttgatc tgaacattca tcacggtgaa atggtggctc tgcttgggcc gtcgggttcc
     1441 ggcaaatcca cccttttacg tcacttaagc ggtttgatta ccggcgataa atccgccggc
     1501 agccatatcg agctgctggg ccgcacagtc cagcgcgaag gccgtctggc gcgcgatatc
     1561 cgcaaaagcc gcgccaacac cggctacatc ttccaacaat tcaacctggt gaaccgcctg
     1621 agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg gcgcacctgt
     1681 tttagctggt tcaccggcga gcagaaacag cgcgcgttac aggcgctgac ccgcgttggc
     1741 atggtgcatt ttgcccatca gcgcgtttcc accctctccg gcggacagca gcagcgtgtg
     1801 gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc tggccgatga acccatcgcc
     1861 tcgctggacc cggaatccgc ccgcatcgtg atggacaccc tgcgcgacat caatcagaac
     1921 gacggcatca ccgtggtcgt cacgctgcat caggtggatt acgccctgcg ctactgcgaa
     1981 cgcatcgtcg ccctgcgcca ggggcacgtt ttctacgacg gcagcagcca acagtttgat
     2041 aacgaacgtt ttgaccatct ctaccgcagc attaatcgca tcgaagagaa cgcgaaagct
     2101 gcctgacatc cccatcattg aggaaaacga atgaacgcta agataattgc ctcgctggcc
     2161 ttcaccagca tgttcagcct cagcaccctg ttaagcccgg cacacgccga agagcaggaa
     2221 aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa accgcaatgg
     2281 acgccattct tacaggatat ggagaagaag ctgggcgtga aggtgaacgc cttctttgcc
     2341 ccagactacg caggcattat ccagggaatg cgcttcaata aagtggatat cgcctggtac
     2401 ggcaacctgt cggcaatgga agcggtggat cgcgccaacg gccaggtctt cgcccagacg
     2461 gtcgcggcgg atggatcgcc aggttactgg agcgtgttga tcgtcaacaa agatagtccg
     2521 atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg caatggcgat
     2581 cctaactcca cctctggctt cctcgtcccc ggttactacg tctttgccaa aaacaatatc
     2641 tccgccagcg actttaagcg caccgtcaac gctgggcatg aaaccaacgc gctggccgtc
     2701 gccaacaagc aggtggatgt tgccaccaac aacaccgaaa acctcgacaa gctgaaaacc
     2761 tccgcgccag agaagctgaa agaactgaag gtgatctgga agtcgccgct gatcccaggc
     2821 gatccgatcg tctggcgtaa aaacctttcc gaaaccacca aagacaagat ctacgacttc
     2881 tttatgaact acggcaaaac gccggaagaa aaagcggtgc tggaacgcct gggctgggcg
     2941 ccgttccgcg cttccagcga cctgcaactg gtgccgattc gccagctcgc gctgtttaaa
     3001 gagatgcagg gcgtgaaaag caataaagga ctgaatgagc aggacaagct ggcaaaaacc
     3061 accgagattc aggcgcagct ggatgacctg gaccgcctga acaacgcgtt aagcgcgatg
     3121 agttcggtga gtaaagcggt gcagtaaatc gtaggtcgga taagacgccc cggcgtcgca
     3181 tccgacaatg tgcaggcgtt gatgccggat gcggtgcaag caccttatcc ggcctacaga
     3241 ccggagccaa acatgcaaac catcaccatc gccccaccca agcgcagctg gttctcgctt
     3301 ctgagctggg ccgttgttct cgccgtgttg gtcgtctcgt ggcagggcgc ggaaatggcc
     3361 ccgctcacgc tgattaaaga cggcggcaac atggcgacgt tcgccgccga cttcttcccg
     3421 cccgatttca gccagtggca ggattacctc accgaaatgg ccgtcacgct gcaaatcgcc
     3481 gtctggggca cggcgctggc ggtggttctc tccatcccct tcggcctgat gagcgccgaa
     3541 aacctggtgc cgtggtgggt ttaccagccc gttcgccgcc tgatggacgc ctgccgcgtc
     3601 attaacgaaa tggtcttcgc catgctgttc gtggtcgccg tcggcctcgg cccgttcgct
     3661 ggcgtgctgg cgctgtttat ccacaccacc ggcgtgctct ccaaactgct ttccgaagcg
     3721 gtggaagcga ttgaacccgg cccggtggaa ggcattcgcg ccaccggcgc caacaagctc
     3781 gaagagatcc tctacggcgt gctgccgcag gtgatgccgc tgctgatctc ctactccctc
     3841 tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg gcatggtcgg tgcaggcggg
     3901 atcggcgtca ccctgtggga agcgattcgc ggtttccagt tccaacaaac ctgcgccctg
     3961 atggtgctta tcatcgtcac ggtcagcctg ctggatttcc tctctcaacg gttgcgtaag
     4021 cactttatct gataagcgag gcattgatat ctatgcactt gtctacacat ccgaccagct
     4081 acccaacacg ctatcaggag atagccgcaa aacttgagca ggagcttcgt caacactacc
     4141 gctgcggcga ctatcttccc gccgagcagc aactggcggc gcgctttgag gtgaaccgcc
     4201 acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg ctgggtacag cgccgtcagg
     4261 gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc gctcaacgcc caggcgcgtt
     4321 ttagccagaa cctgctggac cagggtagcc atcccaccag cgaaaaactg ctgtcggtat
     4381 tgcgccccgc gtccggtcac gtcgctgacg cgctggggat taccgagggc gagaacgtta
     4441 tccatttgcg caccctgcgt cgcgtcaacg gcgtcgcgct ctgtttaatc gaccactact
     4501 ttgcggacct caccctttgg ccgacgctgc aacgcttcga cagcggttcg ctgcacgatt
     4561 ttctgcgcga gcaaaccgga attgcgctgc gccgcagcca gacgcggatc agcgcccgtc
     4621 gcgcccaggc caaagagtgc cagcgtcttg aaattccgaa tatgtcgccg ctgctgtgcg
     4681 tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc ggagtactcc gtcagcctga
     4741 cgcgcgccga catgattgaa ttcactatgg agcactgaat gcacgcagat accgcgaccc
     4801 gccagcactg gatgtccgta ctggcgcaca gccaaccggc tgaactggca gcccgcctga
     4861 aagcactgaa catcaccgcc gactatgagg ttatccgcgc cgccgaaacc ggcctggtgc
     4921 agattcaggc gcggatgggc ggcaccggcg aacgcttttt tgccggcgac gccacgctca
     4981 cccgcgccgc cgtacgcctg actgacggca cgctcggcta cagctgggtg ctggggcgcg
     5041 acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct gatgcagcaa tctcgctatt
     5101 ttcaaaactt atcagaaacc cttattgccc cgctggacgc tgaccgtatg gcacgcattg
     5161 ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt ctttacgatg gttcgcggag
     5221 acaacgcatg accctggaaa ccgcttttat gcttcccgtg caggatgccc agcacagttt
     5281 tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt gtcgccctgc atcagctcaa
     5341 gcgcggctgg cagccgctga atatcgccac caccagcgtg ctgctgacgc tggccgataa
     5401 cgacacgccg gtgtggcttg ccgcgccatt gagtaacgat atcgtcagcc agagcctgcg
     5461 ttttcatacc aacgcgccgc tggtcagcca gccggaacag gcgaccttcg cggtgacgga
     5521 tgaggcgatt tccagcgaac agcttaacgc cctttccacc ggcaccgccg ttgcgccgga
     5581 agcgggtgca acgctgattt tacaggtcgc cagcctgagc ggcgggcgca tgttacgtct
     5641 caccggcgcg ggcattgccg aagaacgaat gatcgctccg cagctgccgg agtgcattct
     5701 gcacgaactc accgagcgcc cgcacccgtt cccgctcggc atcgacctga tcctgacctg
     5761 cggcgagcgc ctgctggcta ttccgcgaac cactcatgtg gaggtgtgct gatgtacgtt
     5821 gccgtgaaag ggggcgagaa ggcgatcgac gccgctcacg ccctgcaaga gagccgacgc
     5881 cggggcgata ccgatttgcc tgaactgagc gtcgcccaga ttgaacagca gcttaatctt
     5941 gcggtggatc gcgtgatgac cgaaggcggc attgccgacc gcgaactggc ggcgctggcg
     6001 ctgaaacagg ccagcggcga taacgtcgaa gcaattttcc tgctgcgcgc ctaccgcacc
     6061 acgctggcga agctcgcggt aagcgagccg ctcgacacca cagagatgcg tctcgaacgg
     6121 cgtatttcgg cggtttataa agacattccc ggcggccagc tgcttggccc aacctacgac
     6181 tacacccatc gcctgctcga tttcaccctg ctggcaaacg gtgaagcgcc gacgctgacc
     6241 accgccgaca gcgaacaaca gccgtcgccg cacgttttca gcctgctggc gcgtcagggg
     6301 ctggtgaagt ttgaagaaga tagcggcgca cagccggatg acatcacccg cacgccgccg
     6361 gtttacccct gctcacgctc ctcccgcctg caacaattaa tgcgcgggga cgaaggctat
     6421 ttgctggcgc tggcctactc cacccagcgt ggttacgggc gcaatcaccc gttcgcgggc
     6481 gagatccgca gtggttacat cgacgtgtcg attgtgccgg aagagctggg atttgcggta
     6541 aacgtcggcg aactactgat gaccgagtgt gaaatggtca acggttttat cgacccgccg
     6601 gatgagccgc cgcacttcac gcgcggctac gggctggtat tcggcatgag cgagcgcaaa
     6661 gcgatggcaa tggcgctggt cgatcgtgcg ttgcaggctc cggaatacgg cgagcacgcg
     6721 acaggcccgg cgcaggatga agagtttgtg ctggcacatg ccgacaacgt cgaagccgca
     6781 ggctttgtct cgcacctcaa actcccccac tacgtcgatt tccaggccga actggagcta
     6841 ctcaaacgtc tgcaacagga gaagaaccat ggctaatctg agcggctaca actttgccta
     6901 cctcgacgag cagaccaaac gcatgatccg ccgcgctatc ttaaaagcgg tggcgatccc
     6961 cggttatcag gtgccgtttg gcgggcgcga gatgccgatg ccatacggct ggggaaccgg
     7021 cggcatacag ctcaccgcca gcgtgattgg cgaaagcgac gtgctgaagg tgattgacca
     7081 gggtgcggat gacaccacca acgccgtgtc gattcgcaac ttctttaagc gcgtgaccgg
     7141 ggtaaacacc actgaacgta cggacgatgc gacgcttatc cagacgcgtc accgcatccc
     7201 cgaaacgccg ctgaccgaag atcagatcat tatcttccag gtgccaatcc cggaaccgct
     7261 gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg cacgcgctgg aagagtacgg
     7321 cgtgatgcag gtgaaactgt atgaagatat cgcccgcttc ggtcatatcg ccactaccta
     7381 tgcctatccg gtgaaggtga acgggcgcta cgtaatggac ccgtcgccga tcccgaaatt
     7441 cgataaccca aaaatggaca tgatgcccgc cctgcaactg ttcggcgcgg ggcgcgagaa
     7501 gcgcatctat gcggtgccgc cgtttacccg cgtggaaagt ctcgatttcg acgatcaccc
     7561 gttcaccgtt cagcagtggg atgagccatg cgccatctgc ggatcgaccc acagctatct
     7621 tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt gtctgctccg ataccgatta
     7681 ttgccgccaa cagagcgagg caaaaaacca atgaatcaac cgttactttc ggtcaataac
     7741 ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg tctcttttga tttatggccg
     7801 ggggaagtgc tgggcattgt cggggagtcc ggctccggga agaccacgct gctgaaatca
     7861 atctccgcgc gcctgacgcc gcagcagggg gagattcact atgagagccg ttcgctgtac
     7921 gcaatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt agtgcatcag
     7981 catccactcg acggcctgcg ccgtcaggtc tcggcaggcg gcaatatcgg cgagcggctg
     8041 atggcgaccg gggcacgtca ttacggcgat attcgtgcca ccgcgcagaa gtggctggaa
     8101 gaggtggaaa ttcccgccaa ccggatcgac gacctgccga ccaccttttc cggcggcatg
     8161 cagcaacgtt tgcagatcgc ccgcaacctg gtgacgcatc cgaagctggt gtttatggac
     8221 gagccgaccg gcggactgga tgtgtcggta caggcacgcc tgctcgacct gctgcgcggc
     8281 ctggtggtgg agctgaacct cgcggtagtg attgtcaccc acgatttagg cgtcgcccgc
     8341 ctgctggcgg accgtttgct ggtgatgaag caggggcaag tggtggagag tgggttaacc
     8401 gaccgcgtgc tcgacgaccc gcaccatccg tatacacaac tgctggtgtc atcggttttg
     8461 cagaattgag ccggtgccgg atgcggcgta aacgccttat ccggcctaca aatgcgctcc
     8521 ccgtaggtcg gataagacgc gtcagcgtcg catccgacac ccgaaccacg aggcgaaaaa
     8581 tgattaacgt acaaaacgtc agtaaaacct tcatcctgca ccagcaaaac ggcgtgcgcc
     8641 tgcccgtctt acaacgtgcc tcgctcaccg tcaacgcggg cgaatgcgtg gtgctccacg
     8701 gccattccgg cagcggcaaa tcaaccttgc tgcgctcgct gtacgccaac tatctgcccg
     8761 acgaagggca aatccagatc aaacacggtg acgaatgggt cgatctggtc accgcgccag
     8821 cgcgcaaagt ggtggagatc cgcaaaacca ccgtcggctg ggtgagccag tttctgcgcg
     8881 tcatcccgcg tatttcagca ctggaagtgg tgatgcagcc gctgctcgat accggcgttc
     8941 cgcgtgaagc ctgcgccgct aaagccgcgc gtctgctcac ccgcctgaac gtgccggaac
     9001 gcctgtggca cctggcgcca tcaacctttt ccggtggcga acagcagcgc gtcaacatcg
     9061 cccgcggctt tatcgtcgac taccccattc tgctgctcga cgaacctacc gcctcgctgg
     9121 acgccaaaaa cagcgccgcc gtagtcgagc tgattcgcga agccaaaacc cgtggcgcgg
     9181 ccatcgtcgg catcttccat gacgaagccg tacgtaatga cgtcgccgac cgcttgcacc
     9241 caatgggagc ctctgcatga ttatcaataa cgtcaaactg gtgctggaaa acgaagtggt
     9301 acacggttcg ctggaggtgc aggatggcga aatccgcgcc tttgccgaaa gccagagccg
     9361 cctgccagaa gcgatggacg gcgaaggtgg ctggctacta cccggcctga ttgagctgca
     9421 taccgacaac ctcgataaat tcttcacccc gcgcccgaaa gtcgactggc ccgcccattc
     9481 ggcgatgagc agccacgacg cgctgatggt ggcaagcggc atcaccaccg tactggacgc
     9541 cgtggcgatt ggcgacgtgc gcgacggcgg cgatcggctg gagaatctgg agaagatgat
     9601 caacgccatc gaagagacgc aaaaacgcgg cgtcaaccgc gccgagcacc gcctgcacct
     9661 gcgctgcgaa ctgccgcatc acaccacact gccgctgttt gaaaaactgg ttcagcgcga
     9721 accggtgacg ctggtgtcgc tgatggacca ctcaccgggc cagcgccagt tcgctaaccg
     9781 cgagaagtat cgcgaatatt atcagggcaa atactccctc accgatgcgc agatgcagca
     9841 gtacgaagaa gagcaattgg cgctcgccgc acgctggtcg cagccgaatc gcgaatccat
     9901 cgccgccctg tgccacgcgc gacaaattgc gctcgccagc cacgatgacg ccacgcacgc
     9961 ccacgttgcc gaatcccacc aacttggcag cgtgatcgcc gaatttccca ccacgttcga
    10021 agcggcggaa gcctcgcgta agcatggcat gaacgtgctg atgggtgcgc caaatatcgt
    10081 gcgtggcggc tcgcactccg gcaatgtggc ggccagtgaa ctggcgcagc ttggtctgct
    10141 ggatatcctc tcttccgact actaccccgc cagcctgctg gatgcggcgt tccgcgtcgc
    10201 cgatgacgag agcaaccgct ttacgctacc gcaggcggtg aggctggtga ccaaaaatcc
    10261 ggcgcaggcg ctgaatcttc aggatcgcgg ggtgattggc gagggtaaac gcgctgacct
    10321 ggtgctggcg catcgccagg gcaatcacat tcatatcgac cacgtctggc gtcagggtaa
    10381 aagggtgttc tgatgatggg aaaactgatt tggttaatgg ggccgtccgg ctccgggaaa
    10441 gacagcctgc tggcggaact ccgcctgcgg gaacaaacgc agttactggt ggcgcatcgc
    10501 tacatcacgc gcgacgccag cgccggaagt gaaaaccata tcgccctgag tgagcgggag
    10561 ttttttaccc gcgcggggca aaacctgctg gccttaagct ggcacgctaa cggtctgtat
    10621 tatggcgtcg gcatcgagat tgacctctgg ctgcacgctg gattcgacgt agtcgtcaac
    10681 ggttcacgcg cgcatttgcc gcaggcacgg gcgcgctatc aatcggcgct gctgcccgtc
    10741 tgtttacagg tttcgccgga gatcctgcgc cagcgcctgg aaaaccgtgg ccgtgaaaac
    10801 gccagtgaaa ttaacgcccg gctggcgcgc gccgcccgct atactccaca ggattgccat
    10861 acgctcaaca atgacggcag cctgcgccag tcggtcgaca cactgctgac gctgattcat
    10921 cagaaggaga aacaccatgc ctgcttgtga gcttcgcccg gccacgcagt acgataccga
    10981 cgcggtttac gcgctgattt gtgagctaaa acaggcggag tttgaccacc acgcgtttcg
    11041 cgtgggcttt aacgccaacc tgcgcgaccc gaacatgcgc taccatctgg cgctgctgga
    11101 tggcgaagtt gtcggcatga tcggcctgca tttgcagttt catctgcatc atgtcaactg
    11161 gatcggcgaa attcaggaac tggtggtgat gccgcaggcg cgcggcctga acgtcggtag
    11221 caagttgctg gcgtgggcag aagaagaagc ccgccaggcc ggggccgaaa tgaccgagct
    11281 ttcgaccaac gtgaagcgcc acgacgcgca ccgtttctat ctgcgagaag gctacgagca
    11341 gagccacttc cgcttcacca aggcgctgta acatgagcct gaccctcacg ctcaccggca
    11401 ctggcggcgc acagggcgtt ccggcatggg gctgcgagtg cgcggcctgc gccagagcgc
    11461 ggcgctcgcc gcagtatcgc cgccagccat gcagcggcgt agtgaagttt aacgacgcga
    11521 tcaccctgat cgacgccggg cggcacgatc tcaccgatcg ctggtcgcct ggatcgttcc
    11581 agcagttttt gctgacgcac taccatatgg atcacgtcca ggggctattt ccgctacgct
    11641 ggggcgtggg cgatgtgatc ccggtctacg gcccgccgga tgaacagggc tgcgacgatc
    11701 tgtttaaaca tccgggcctg cttgatttca gccacacagt ggaaccgttt gtggtgtttg
    11761 atttgcaggg gttgcaggtc acgccgctgc cgcttaacca ctcaaaactg accttcggtt
    11821 atctgctgga aacggcacat agccgggtgg cgtggctgtc tgacaccgct ggcctgccgg
    11881 aaaaaacgct gaaattttta ctcaataatc atccgcaggt gatggtgatt gattgcagtc
    11941 acccgccgcg cgcggatgca ccgcgtaatc actgtgattt aaataccgtg cttgcgctga
    12001 atcaggttat ccgctcgcca caggtgattc tgacccatat cagccaccag tttgatgcgt
    12061 ggctgatgga aaacgcacta ccgtcagggt ttgaggtggg gtttgatggg atggagattg
    12121 gggtggcgtg atgagaggga atgtgcgcgc tggccccctc accctaaccc tctccccaga
    12181 ggggcgaggg gaccgattgt gctcaatatt gaatattgcg ctcgttttct ccctctcccc
    12241 attggggtga ggggcgatgc ctgctccata cccaacctca tcgcccatac tcatcttcca
    12301 ttctccgctc ttcatcctcc agttgccgac gctcctgatc aagctggcgc tggcgatcgt
    12361 ccagctgcct gcggcgatct tcaaactggc ggcggcggtc gtcatattgt ctgcgccgat
    12421 cgtcgctcac ttcacgctgc cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt
    12481 cagggttata agcgtcatta atcgcctgct gaatattacc aatagtatcg tcgataatat
    12541 cagcctgggc cggaacggtg gacagcgtga gcagggtgaa taaaagaaat agcggaaagc
    12601 gtttcattag ccaacctcaa aaagaaactc tatccacatt agtcattact catccatgca
    12661 agtagtggat gaatctcaat ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg
    12721 ttgatgacga ccgccttcgt actgtgcgcc cagccacgca tccacaatca tttttgccag
    12781 ttcgaggcca accactcgtg aaccaaaagc cagtgcaact tcactggcat aatgcggata
    12841 atcagtgcgc tctgacgacc tggtgtcttt atcaatcacc tcactcattg accatgaata
    12901 aaaggctggc acagcaacac agaatcctgc ggtgccagcg acatgaccgc ccattgctcc
    12961 ggtacgtttt ttaaaaagaa acaccggttg cattgataaa ttgtgctcag tatttgccga
    13021 agtacaatat atataataaa tatataaaac acctcatatt atttacagag ataataaatt
    13081 cgcgtaattt tcccccttca gcacaaatgc ctaataaaat ggatcgttcc gctcacactt
    13141 ccacttatta aagtcgcaaa tacccaatta tgataacgcc agctttaaat gacgtatttt
    13201 gcatggatta catatttacg acacaaatga taaatggaga tttatatgaa taactctcgg
    13261 ttattccgtt tgagcaggat tgttattgcg ttaactgctg ccagcggcat gatggtaaat
    13321 accgctaacg cgaaagagga agcgaaagcc gccactcaat atacccaaca ggttaatcag
    13381 aattacgcca aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga
    13441 tttatcgctc cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat
    13501 cgcggggaag attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc
    13561 ctgtggcgtc agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg
    13621 taccaggttc gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt
    13681 attgtcatcg acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc
    13741 caaaatcgcc cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac
    13801 tatggcggcg tgaaaggcat tatctctgaa gctgatgtga aatccggtaa agtgcaggtt
    13861 atcgcccctg ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt
    13921 atgagccgcc gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac
    13981 atcggcaatg gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac
    14041 aaaaccatca ccaaaaccgg tgagaagatg attatcgacg gcctagagtt tgacttcctg
    14101 atgaccccag gtagcgaagc accagccgaa atgcacttct atattccggc cctgaaagcg
    14161 ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact tctacactct acgcggcgcg
    14221 aaaacccgcg acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt
    14281 aacgacgcgg aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc
    14341 aatgattata ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac
    14401 ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca
    14461 cttgccaata actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg
    14521 gtgtataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt
    14581 caggtggaga tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac
    14641 ctggcgcaag aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag
    14701 gtgattgccg ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa
    14761 cagctgggct atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa
    14821 gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt
    14881 cgcgggatgt cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa
    14941 gccgcgggta aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg
    15001 acgctgaacg atagcgtgct taactaccgt aaaacactgc aatcccaagc tgacgcctct
    15061 ttctacatca gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat
    15121 ctggtaaaag cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc
    15181 gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg
    15241 cttt
//
LOCUS       DAZFDP010000119        15143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFDP010000119 DAZFDP010000000
VERSION     DAZFDP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHJ1G_003674"
     CDS             56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHJ1G_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO4765452.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPYYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHJ1G_003675"
     CDS             complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHJ1G_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4765453.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1820..4402
                     /gene="leuS"
                     /locus_tag="ACHJ1G_003676"
     CDS             1820..4402
                     /gene="leuS"
                     /locus_tag="ACHJ1G_003676"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4765454.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4417..4998
                     /gene="lptE"
                     /locus_tag="ACHJ1G_003677"
     CDS             4417..4998
                     /gene="lptE"
                     /locus_tag="ACHJ1G_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4765455.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4998..6029
                     /gene="holA"
                     /locus_tag="ACHJ1G_003678"
     CDS             4998..6029
                     /gene="holA"
                     /locus_tag="ACHJ1G_003678"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4765456.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6031..6672
                     /gene="nadD"
                     /locus_tag="ACHJ1G_003679"
     CDS             6031..6672
                     /gene="nadD"
                     /locus_tag="ACHJ1G_003679"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4765457.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6696..7307
                     /gene="cobC"
                     /locus_tag="ACHJ1G_003680"
     CDS             6696..7307
                     /gene="cobC"
                     /locus_tag="ACHJ1G_003680"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4765458.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHJ1G_003681"
     CDS             7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHJ1G_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4765459.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHJ1G_003682"
     CDS             7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHJ1G_003682"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4765460.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHJ1G_003683"
     CDS             8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHJ1G_003683"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4765461.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHJ1G_003684"
                     /gene_synonym="rodA"
     CDS             10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHJ1G_003684"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4765462.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHJ1G_003685"
     CDS             11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHJ1G_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4765463.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12641..13852
                     /gene="dacA"
                     /locus_tag="ACHJ1G_003686"
     CDS             12641..13852
                     /gene="dacA"
                     /locus_tag="ACHJ1G_003686"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4765464.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHJ1G_003687"
     CDS             13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHJ1G_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4765465.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14327..14968
                     /gene="lipB"
                     /locus_tag="ACHJ1G_003688"
     CDS             14327..14968
                     /gene="lipB"
                     /locus_tag="ACHJ1G_003688"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4765466.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3748 a         3843 c         3947 g         3605 t
ORIGIN      
        1 aaatggaaca cataaaaata taatatgttt tatctgcaac cataaggaaa tgataatgat
       61 tttcacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
      121 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
      181 ttctccggac gacgaaaaag ccttttactg gctgaagctg gccgctgagc aaggtcattg
      241 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
      301 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
      361 cgcccttggc tggacactgg atcgtggaga agccccctat tataaagaag cggttgtctg
      421 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta
      481 cagaaatggc aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc
      541 tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa
      601 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
      661 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
      721 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
      781 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
      841 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
      901 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
      961 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     1021 agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt
     1081 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac
     1141 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     1201 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     1261 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgata tctgccagct
     1321 cctgccataa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     1381 tctcttcata gctcatggcg aactcttcca ggtcacgtct tacagctcgc gtcagctcat
     1441 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     1501 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac
     1561 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     1621 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
     1681 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     1741 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     1801 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     1861 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     1921 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     1981 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
     2041 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     2101 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     2161 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     2221 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
     2281 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     2341 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     2401 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     2461 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     2521 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     2581 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     2641 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     2701 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     2761 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     2821 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     2881 gaacatcaaa ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc
     2941 cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc
     3001 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     3061 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
     3121 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
     3181 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     3241 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     3301 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     3361 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     3421 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     3481 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     3541 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     3601 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     3661 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     3721 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     3781 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     3841 gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     3901 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
     3961 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     4021 caaactggcg aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct
     4081 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
     4141 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     4201 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     4261 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     4321 ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac caggtaaact
     4381 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
     4441 ttatctctgg ctgtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
     4501 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     4561 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     4621 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     4681 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
     4741 ttgatccccg gccgtgatat ctacccgatt agtgccaaag tcttccgttc gttctttgat
     4801 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     4861 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     4921 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     4981 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     5041 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     5101 aggacgctgt tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca
     5161 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     5221 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     5281 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta
     5341 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
     5401 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg
     5461 caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc tactgttatg
     5521 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
     5581 gcaaattgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     5641 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     5701 aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt
     5761 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     5821 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc
     5881 agacgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     5941 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     6001 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
     6061 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
     6121 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     6181 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     6241 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     6301 gagtggcggc aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca
     6361 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     6421 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     6481 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     6541 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     6601 ggtgaatcgt gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc
     6661 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     6721 gtgaaacgca agcgaatatc gatggtcttt acagcggtca tgcgcccacc cccctgaccg
     6781 cgcgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc
     6841 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgacgggtt ctcagtgacc
     6901 gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     6961 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     7021 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     7081 aaagatttat cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc
     7141 atcagggcgt actgagtctg ttaattgctc gtctaattgg catgcctgcc gaatctatgt
     7201 ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
     7261 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
     7321 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
     7381 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
     7441 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
     7501 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
     7561 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
     7621 ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca gcatcaccga ctgcatgatc
     7681 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
     7741 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
     7801 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
     7861 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
     7921 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
     7981 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
     8041 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
     8101 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
     8161 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
     8221 tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
     8281 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
     8341 tatcaccgtg agtgataaag gtgctttgag tagaaaacgc agcggatgaa actacagaac
     8401 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
     8461 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
     8521 cgctttaccg attaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg
     8581 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggcactcaa ccgcactatc
     8641 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
     8701 cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
     8761 tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca agtagctcgc
     8821 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
     8881 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
     8941 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
     9001 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
     9061 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
     9121 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
     9181 ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggca
     9241 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
     9301 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
     9361 tatcctcctg cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg
     9421 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa
     9481 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
     9541 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
     9601 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
     9661 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
     9721 ggtgacacca ttccggttgg tatcggccag ggttactgga cagcgacccc aatccagatg
     9781 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
     9841 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc
     9901 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
     9961 cctaacggta cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct
    10021 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    10081 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    10141 gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc
    10201 cagatcctcg accacattat gctgggtgat aacaataccg atctgcctgc ggaaaatcct
    10261 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    10321 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    10381 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatag
    10441 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    10501 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    10561 ctttcggtgc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    10621 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    10681 acgtttgccc gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca
    10741 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    10801 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    10861 tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    10921 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    10981 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat ggcactcagt
    11041 cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    11101 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    11161 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa
    11221 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    11281 ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt gtgctgatgg
    11341 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    11401 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    11461 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    11521 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    11581 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    11641 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    11701 tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct tccgatcccc
    11761 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    11821 cgcggcccct acggcaacga ccgcgtaatt tcgctttctc gcgcagcagc tgaccgtctt
    11881 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    11941 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    12001 cctcccgatt taagcggtgg cgcgggggca agttcagtgt ctggcccgca gggtgacatt
    12061 cttccggtca gtaactcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    12121 agcggtttcc tcggcgctcc aacgacctta gcgcctggtg tactggaaag cagcgaaccg
    12181 acacctgcac cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca
    12241 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    12301 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    12361 gtccccggtc gcgtaactca aaatggcgcg gtctggcgta tccagcttgg cccattcgcc
    12421 agtaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    12481 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    12541 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    12601 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    12661 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    12721 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    12781 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    12841 ccgccgcgat cctgctagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    12901 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    12961 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    13021 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gcgtcgccat
    13081 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    13141 cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg ctgatggtca
    13201 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    13261 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    13321 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    13381 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    13441 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    13501 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    13561 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    13621 gaccattcca cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    13681 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    13741 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    13801 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    13861 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    13921 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    13981 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    14041 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    14101 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    14161 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    14221 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    14281 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    14341 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    14401 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    14461 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    14521 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    14581 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    14641 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    14701 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    14761 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    14821 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    14881 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    14941 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    15001 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    15061 tcacttgcag aaatgcggtg ccacgccttg ctccgcaact caccccattg attaacctat
    15121 tgaaatagtt gattatggtg agc
//
LOCUS       DAZFDP010000120        15106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFDP010000120 DAZFDP010000000
VERSION     DAZFDP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..1214
                     /gene="citC"
                     /locus_tag="ACHJ1G_003689"
     CDS             156..1214
                     /gene="citC"
                     /locus_tag="ACHJ1G_003689"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4765467.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1229..1525
                     /gene="citD"
                     /locus_tag="ACHJ1G_003690"
     CDS             1229..1525
                     /gene="citD"
                     /locus_tag="ACHJ1G_003690"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4765468.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1522..2430
                     /gene="citE"
                     /locus_tag="ACHJ1G_003691"
     CDS             1522..2430
                     /gene="citE"
                     /locus_tag="ACHJ1G_003691"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4765469.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2441..3973
                     /gene="citF"
                     /locus_tag="ACHJ1G_003692"
     CDS             2441..3973
                     /gene="citF"
                     /locus_tag="ACHJ1G_003692"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4765470.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3977..4528
                     /gene="citX"
                     /locus_tag="ACHJ1G_003693"
     CDS             3977..4528
                     /gene="citX"
                     /locus_tag="ACHJ1G_003693"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4765471.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4503..5381
                     /gene="citG"
                     /locus_tag="ACHJ1G_003694"
     CDS             4503..5381
                     /gene="citG"
                     /locus_tag="ACHJ1G_003694"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4765472.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5432..6895
                     /gene="citT"
                     /locus_tag="ACHJ1G_003695"
     CDS             5432..6895
                     /gene="citT"
                     /locus_tag="ACHJ1G_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4765473.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7009..7815
                     /gene="rna"
                     /locus_tag="ACHJ1G_003696"
     CDS             7009..7815
                     /gene="rna"
                     /locus_tag="ACHJ1G_003696"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4765474.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8045..8455
                     /gene="rnk"
                     /locus_tag="ACHJ1G_003697"
     CDS             8045..8455
                     /gene="rnk"
                     /locus_tag="ACHJ1G_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4765475.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8593..8682
                     /gene="yldA"
                     /locus_tag="ACHJ1G_003698"
     CDS             8593..8682
                     /gene="yldA"
                     /locus_tag="ACHJ1G_003698"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4765476.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHJ1G_003699"
     CDS             complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHJ1G_003699"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4765477.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10145..10573
                     /gene="uspG"
                     /locus_tag="ACHJ1G_003700"
     CDS             10145..10573
                     /gene="uspG"
                     /locus_tag="ACHJ1G_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4765478.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHJ1G_003701"
     CDS             complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHJ1G_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4765479.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12430..12993)
                     /gene="ahpC"
                     /locus_tag="ACHJ1G_003702"
     CDS             complement(12430..12993)
                     /gene="ahpC"
                     /locus_tag="ACHJ1G_003702"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4765480.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13365..14111
                     /gene="dsbG"
                     /locus_tag="ACHJ1G_003703"
     CDS             13365..14111
                     /gene="dsbG"
                     /locus_tag="ACHJ1G_003703"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4765481.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14320..>15106
                     /locus_tag="ACHJ1G_003704"
     CDS             14320..>15106
                     /locus_tag="ACHJ1G_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4765482.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLK"
BASE COUNT         3672 a         3942 c         3739 g         3753 t
ORIGIN      
        1 gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt
       61 tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat
      121 cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc
      181 gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg
      241 atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta
      301 tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc
      361 gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc
      421 acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg
      481 gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac agcgccacgc
      541 ggctgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct
      601 gcattgtgat gaacgccaat cccttcacga atggtcaccg ttatctgatt caacaagctg
      661 cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct
      721 atgaagaccg gttggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc
      781 atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac
      841 agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg
      901 ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg
      961 cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc gcaccgccca
     1021 tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg
     1081 tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca
     1141 cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa
     1201 agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac
     1261 ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct
     1321 gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga
     1381 cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact
     1441 ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc
     1501 tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc
     1561 cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc
     1621 tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac
     1681 accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc
     1741 attgtgcgtg tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt
     1801 cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat
     1861 attgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg cagcaccggc
     1921 ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct
     1981 tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca
     2041 gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg
     2101 cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga
     2161 ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac
     2221 ccgcgtcaga ttgatctgtt gcacaacctc tacgcaccaa cccagaaaga agtggatcac
     2281 gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc
     2341 ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctctcgt
     2401 gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca
     2461 atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc
     2521 tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg
     2581 cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga cggtttcctt
     2641 ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa
     2701 gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgtt
     2761 ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc tgcgcggtcc
     2821 actggcggaa gagatctccc gtggtctgct ggcagaaccg gtgcaaatcc actctcacgg
     2881 cggtcgcgtg catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt
     2941 cccgtcctgc gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc
     3001 cctcggctat gcaatggttg atgccgacaa cgcaaagcag gtcgtgatgc ttaccgaaga
     3061 actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt
     3121 caaagttgac cgcgttggcg atgctgcaaa aatcggcgct ggcgcgaccc gtatgaccac
     3181 taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca actcaggcta
     3241 cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg cggtaacccg
     3301 tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc ttggcggtat
     3361 taccgcgacg atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca
     3421 gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag
     3481 cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt
     3541 ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga
     3601 cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc
     3661 catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata acgtgctgac
     3721 ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa
     3781 cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag tggtttccat
     3841 tgagtggctg cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac
     3901 cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca
     3961 ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat
     4021 tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct ggctcaagcg
     4081 ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga
     4141 ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag ccgcaaaaca
     4201 gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt
     4261 gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca
     4321 tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg
     4381 ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg
     4441 cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa
     4501 cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg
     4561 agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag
     4621 gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc
     4681 gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg
     4741 cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag
     4801 gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag
     4861 ggctgctatg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggta acgccaacaa
     4921 ccgtttgttc tacggcggca agtttctgcc gtggtctgac cgatcgcgaa ctgcgtacca
     4981 ataattcaca actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac
     5041 gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg cattacctca
     5101 ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga
     5161 tggcgatcaa cggcgatacc aacgttgctt cgcgcggtgg cgaggggggc ctgcgctggc
     5221 tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc gccgatctcg
     5281 attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg
     5341 ccgacctgct gatccttacc tggtttttag cacagattta attatttaag cacttgataa
     5401 atttgaaaat attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa
     5461 actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat
     5521 gccaccacag gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct
     5581 cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag
     5641 taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca
     5701 ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc
     5761 atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct
     5821 ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat
     5881 tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt
     5941 cattaaaaac ctgccgccgt tgtttaaatc attccccaac gatccgtccg cgcgtcgtat
     6001 tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt
     6061 caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca
     6121 gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat
     6181 tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc
     6241 aacctgggcg ggtgatgaac tgaaaaccat gggtgcgctg acacgcagag agtggacact
     6301 gattggcctt gtattgctca gcttaggttt gtgggttttt ggcagcgaag tcattaatgc
     6361 tactgcagtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg tgccttggaa
     6421 agatattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt
     6481 gatggctaac ggcctgactc gttctggctt tattgactgg ttcgccggta ccatgagtac
     6541 gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt
     6601 tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct
     6661 ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct
     6721 gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta
     6781 cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat
     6841 ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt
     6901 tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt
     6961 atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc
     7021 tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc caacgcctta
     7081 gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa
     7141 accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg
     7201 caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg gccaggattg
     7261 cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct
     7321 actcgcccaa tcccgaatct accagaagcg cacgccagcc ggatgtgttc atcgccggaa
     7381 accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga
     7441 cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg
     7501 gacgcatact tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga
     7561 aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt
     7621 gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct
     7681 gcgtatttga ctgaaattca gatctcgatc aaagctgacg caatcaacgc tccgctttct
     7741 gcaaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt tgtgattgat
     7801 aaagtgggtt attaatcatc tgatttacaa aacacgctaa ttcgtcttcc ataaaccgtg
     7861 acgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc
     7921 acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg
     7981 gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt
     8041 aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct
     8101 gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga
     8161 tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg
     8221 ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc
     8281 aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct
     8341 ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct
     8401 tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca
     8461 tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct ttacgcaaaa
     8521 tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt
     8581 gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg
     8641 attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgatcata ttgttccccc
     8701 cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc
     8761 gggtaccaga atcaccttac ggcactcctc ttcacgtttt tcgaaaatct catatccccg
     8821 ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt
     8881 ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa
     8941 cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac
     9001 gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc gcaacgcttt
     9061 accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc
     9121 ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat
     9181 aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc
     9241 agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag
     9301 caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga ctgcaacgct
     9361 tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca gaatatcaga
     9421 aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc
     9481 tttagggacg cggacatatt ccgcctgtcc accaggaacg ccaccataca ggtgactata
     9541 actaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc ctttacccgc
     9601 attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt cgccacaagc
     9661 aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt cctttccggt
     9721 ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
     9781 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa tacgcagaat
     9841 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
     9901 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
     9961 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattcctg
    10021 gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa
    10081 tatcagggat aaatattata atcatggcga atgatttgtt catgattaac agggagaaag
    10141 ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
    10201 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
    10261 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
    10321 agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat
    10381 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
    10441 gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc catcgatttc
    10501 gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc tgccggtgct
    10561 ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc ccggcagggc
    10621 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
    10681 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
    10741 ctttggcacc ttcgcctgtg gcgatgataa tctgcttgta cggaaccgtc gtacagtcac
    10801 ccgctgcgaa cacgcctttg acgttggttt cgcatttcgc atcaatgata atctcaccca
    10861 tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga
    10921 cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct cgatattcca
    10981 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
    11041 cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg
    11101 caaattcgag cagcgttacg tgctcaacga tacccgccag gtcaattgcc gcttccacgc
    11161 cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg ccgtcgcagt
    11221 gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gccaggaacg ttcatgttgc
    11281 gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
    11341 tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg gcgctctggc
    11401 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccagcttct
    11461 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
    11521 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
    11581 cagctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca
    11641 gctcttctgc cgcacgtttt tccgcgcccg tatcaatttt ggcaacgatt tcagtcaacg
    11701 tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt
    11761 tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
    11821 ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga
    11881 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
    11941 actgcgcttc tttcgacgga tgaccaccgg tccatagcaa cgccagtacc agcgaggtga
    12001 actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct gggttggtga
    12061 tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg actttgtctg
    12121 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
    12181 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
    12241 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
    12301 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc
    12361 acccgaagaa ttaggtgaat ttccgaagta tttcgcgccg gagttggtag cgcgaaagac
    12421 gaaggaaatt tagattttac caaccaggtc cagagacgga gccagagttg cttcaccttc
    12481 tttccattta gccgggcaaa cttcacctgg gtgagaagct acgtactgtg ctgctttgat
    12541 tttacgcagc aggtcagacg cgtcacggcc aatgccttca gcggtaactt cgattgcctg
    12601 gatgataccc tgcgggtcaa caacgaaggt cgcacggtca gccagacctt catcttcacg
    12661 catgttgtcg aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg catatttgat
    12721 tttagcgatg gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag tatcggtaga
    12781 tactgcgtat acgtctacgc ccagtttctg cagttcttcg tagtggtcag caacgtcacc
    12841 cagttcggtc gggcatacga aagtaaagtc agccgggtag aagaagaaga cgctccagcg
    12901 gccttcggta tctttttcgg tgatttcgat gaattcgccg tttttgaatg cctggttttt
    12961 aaaaggttta attttggtgt taatcaagga catctatact tcctccgtgt tttcgatgag
    13021 atgtaaggta accaattttt gccgattcgg ctaatgcgtt tccattatca aatcgataag
    13081 ttttacctta caaccttcgt aagacaactt tgtgaacttt gcaccgccag cggcgataaa
    13141 aagtaaaatg gccccctttc caggtgaccg tgtaatctga actacccttt ggtatcttca
    13201 gtgcctgcct gcaggctgag gtgttgccct tacaaatgca acaacgacat ggattacaac
    13261 accctcataa acaaagggca atcacctgat ctaagctctt acctatgaca gtgataggtt
    13321 atgcctttta ctcgactttt gcactgactg aaaaggacaa attaatgtta aaaaagatac
    13381 ttttactggc tctgcttcct gcaatcgcct tcgcagagga acttcctgct ccagtaaaag
    13441 cgattgaaaa acagggcatt acaatcatca aaacattcga tgcccccgga ggaatgaaag
    13501 gttatctcgg aaagtatcag gatatgggcg tcaccatcta cctgactcca gatggtaagc
    13561 acgctatctc tggttacatg tacaacgaga aaggtgaaaa cctgagtaac acacttatcg
    13621 aaaaagaaat ttacgcacca gccggacgcg aaatgtggca acggatggaa caatcccact
    13681 ggctcctcga cggtaaaaaa gatgcgccgg tcattgtcta cgtgttcgcc gatccgttct
    13741 gcccatattg taaacagttc tggcagcagg cgcgcccgtg ggtagattct ggcaaagtgc
    13801 aattaagaac attgttggtt ggggttatca agccagaaag cccggcgaca gcagcggcaa
    13861 ttcttgcctc caaagatccc gcaaaaacct ggcaagaata tgaatcctct ggtggcaagc
    13921 ttaagctaaa cgtgcctgca aacgtaagta cagaacaaat gaaagtgtta agcgccaacg
    13981 agaaactcat ggacgatctg ggggcaaatg tcacaccagc gatctattac atgagtaagg
    14041 aaaatacgct gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat atcattatgg
    14101 ggaataaata aaagataatt catggatggt tgaaatacat ccctgctttt caaccatcct
    14161 aaaaaagcat gaattgctcg ctctgcccta agtggctatg ctattttaga aaaacttaat
    14221 cctgagttaa gcaaaaaaat ataatcaatg aaaatatata cgcaaaaaac attgattaag
    14281 tgaatatacc atggaagaaa aatataaccg gagtagtgta tggccaatct ctacgacttg
    14341 aaaaagttcg acttaaacct tcttgtcata tttgagtgta tttaccagca tttaagcatt
    14401 agcaaagctg ctgaatcact atatatcacc ccatcagccg tgagccagtc gttacaacgc
    14461 ttacgtgcgc aatttaatga ccctttattc attcgctcag gaaaaggtat cgcgccgacg
    14521 acaacagggt tgaatctgca tcaccatctt gagaaaaacc tcaaaggcct tgaacaaaca
    14581 attaatatag tcaataagtc cgaactaaag aaaaacttta ttatttacgg ccctcagctc
    14641 atttcttgct ctaataatag tatgctgatc cgctgcctac ggcaagatgc ctctgtcgag
    14701 attgagtgcc atgatatcct catgtcagca gagaatgctg aagaactgtt ggttcaccgc
    14761 aaagccgatc tggtgatcac ccaaatgcca gtgatcagcc gttccgtcat ttgcatgcca
    14821 ttgcatacaa ttcgcaatac tctaatttgt agtaataagc atccgcgaat tactgacaat
    14881 agcacttatg aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga
    14941 gttgatgata ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt
    15001 ggttcctctt tgctgacaat cattaatagc attgcagtta cagatttact gggaatagtc
    15061 ccctatgagc tttataattc ctaccgtgac tttcttaatt taaaag
//
LOCUS       DAZFDP010000121        15040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFDP010000121 DAZFDP010000000
VERSION     DAZFDP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..1355
                     /gene="hpaG"
                     /locus_tag="ACHJ1G_003705"
     CDS             66..1355
                     /gene="hpaG"
                     /locus_tag="ACHJ1G_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO4765483.1"
                     /translation="MKGTIFVVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1352..2818
                     /gene="hpaE"
                     /locus_tag="ACHJ1G_003706"
     CDS             1352..2818
                     /gene="hpaE"
                     /locus_tag="ACHJ1G_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4765484.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2820..3671
                     /gene="hpaD"
                     /locus_tag="ACHJ1G_003707"
     CDS             2820..3671
                     /gene="hpaD"
                     /locus_tag="ACHJ1G_003707"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO4765485.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3681..4061
                     /locus_tag="ACHJ1G_003708"
     CDS             3681..4061
                     /locus_tag="ACHJ1G_003708"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO4765486.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4172..4975
                     /gene="hpaH"
                     /locus_tag="ACHJ1G_003709"
     CDS             4172..4975
                     /gene="hpaH"
                     /locus_tag="ACHJ1G_003709"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO4765487.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4986..5774
                     /gene="hpaI"
                     /locus_tag="ACHJ1G_003710"
     CDS             4986..5774
                     /gene="hpaI"
                     /locus_tag="ACHJ1G_003710"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO4765488.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5949..7325
                     /gene="hpaX"
                     /locus_tag="ACHJ1G_003711"
     CDS             5949..7325
                     /gene="hpaX"
                     /locus_tag="ACHJ1G_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO4765489.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7335..8225
                     /gene="hpaA"
                     /locus_tag="ACHJ1G_003712"
     CDS             7335..8225
                     /gene="hpaA"
                     /locus_tag="ACHJ1G_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO4765490.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8476..10038
                     /gene="hpaB"
                     /locus_tag="ACHJ1G_003713"
     CDS             8476..10038
                     /gene="hpaB"
                     /locus_tag="ACHJ1G_003713"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO4765491.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10056..10568
                     /locus_tag="ACHJ1G_003714"
     CDS             10056..10568
                     /locus_tag="ACHJ1G_003714"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO4765492.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10961..13111
                     /gene="btsT"
                     /locus_tag="ACHJ1G_003715"
     CDS             10961..13111
                     /gene="btsT"
                     /locus_tag="ACHJ1G_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4765493.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13242..13445
                     /locus_tag="ACHJ1G_003716"
     CDS             13242..13445
                     /locus_tag="ACHJ1G_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4765494.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13456..14412
                     /gene="yjiA"
                     /locus_tag="ACHJ1G_003717"
     CDS             13456..14412
                     /gene="yjiA"
                     /locus_tag="ACHJ1G_003717"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4765495.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14499..14690)
                     /locus_tag="ACHJ1G_003718"
     CDS             complement(14499..14690)
                     /locus_tag="ACHJ1G_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO4765496.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14730..>15040)
                     /locus_tag="ACHJ1G_003719"
     CDS             complement(14730..>15040)
                     /locus_tag="ACHJ1G_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HFO4765497.1"
                     /translation="GAFIVSTILVAFASKNVEGISYYVLKSTIDFEWRNQVKCGELNI
                     SRPDERYFGFNTDKYTVFYSNREGKWGVNELKCKKGSDRRDAYSIENVSEYNVPGWLK
                     "
BASE COUNT         3502 a         4116 c         4097 g         3325 t
ORIGIN      
        1 caatcgggaa taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag
       61 tggtaatgaa aggcactatc ttcgtcgtag cgttgaacca tcgcagccaa cttgatgcat
      121 ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg gtctggttta
      181 ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa
      241 aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg aaagtacgtg
      301 aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc agcctgccgg
      361 aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg
      421 gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag atcaacgggc
      481 gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag ttgctgagcg
      541 ccctgagcga atttgccaca ctgaatccag gcgatgccat tctgctcggc acgccacagg
      601 cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt ttcccgccgc
      661 tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgcagc
      721 cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac
      781 tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa agcgccaaat accctcactg
      841 gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat gaagcggagc
      901 tggtggtagt tattggcaaa caggcgcgta acgtcagcga agccgatgcc atggattatg
      961 tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc
     1021 gccctaacct gcgggtaaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc
     1081 cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt
     1141 tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact
     1201 taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct
     1261 tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggctgcctgg
     1321 tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg
     1381 caaaaatgtt gcaggtaacg actacttcca gaccaccaat ccggcaacgg gtgaagtgct
     1441 ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga
     1501 ggcgttcccg aaatgggcca atctgccgat gaaagaacgt gcgcgcctga tgcgccgtct
     1561 gggcgatctg atcgaccaga acgtgccaga gatcgccgcg atggaaaccg cggacaccgg
     1621 cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttcccaca actttgaatt
     1681 tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct
     1741 caactacacg ctggtgcagc cggtgggcgt ttgtgcgctg gtatcgccgt ggaacgtacc
     1801 gtttatgacc gccacatgga aggtcgcgcc gtgtctggcg ctgggcaata ctgcggtgct
     1861 gaagatgtca gaactctccc cgctgaccgc tgaccgcctg ggtgagctgg cgctggaagc
     1921 cggtattccg gcgggcgtgc tgaacgtggt acagggctac ggcgcaaccg caggggatgc
     1981 gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg
     2041 caacatcatg aaaaacgccg ggctgaaaaa atactccatg gaactgggcg gaaaatcgcc
     2101 agtgctgatt tttgaagatg ccgatattga acgcgcgctg gacgccgccc tgttcaccat
     2161 cttctcgatc aacggcgagc gctgcaccgc cggttcgcgc atctttattc agcaaagcat
     2221 ctacccggaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc
     2281 gaacgatccg aatacccagg ttggggcact tatcagccag caacactggg aaaaagtctc
     2341 cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ctggcgggcg gcccggataa
     2401 accgtctgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc
     2461 agacgtagat aaccgtatgc gcgtcgccca ggaagagatt ttcgggccgg tcgcctgcct
     2521 gctgccgttt aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct
     2581 cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga
     2641 agcaggcatg gtgttcgtca atacccagaa cgtccgcgac ctgcgccagc cgtttggcgg
     2701 cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga
     2761 aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata
     2821 tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac
     2881 taccaggaaa aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca
     2941 agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgacacccac tggctggtca
     3001 acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc agtaacgaac
     3061 tgccgcattt tattcgtgac atgacctaca actacgaggg caacccggag ttggggcagc
     3121 taattgccga tgaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc
     3181 tgaaactgga atacggcacg ctggtgccga tgcgctacat gaatgaagac aagcacttca
     3241 aagtggtctc catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg
     3301 gcgaagcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg
     3361 gttcgttatc gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca
     3421 cccgcgagtt cgaccgccag atggacgagc gcgtggtgaa gctgtggcgc gaaggccagt
     3481 tcaaagagtt ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata
     3541 tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg
     3601 agtttatcac cgagctattc ccaagctctg gcaccggtca ggttaacgct gttttcccgc
     3661 ttcccgcgta aggacgtttt atgccgcact ttatcgttga atgcagtgat aacatccgcg
     3721 aagaagccga cctgccgggg ttgttcgcca aagtgaatcc gacgctggca gccacgggta
     3781 tttttccgct ggcgggtatt cgcagccgcg tgcattgggt cgatacctgg cagatggccg
     3841 acgggcagca tgattatgcc ttcgtgcata tgacgttgaa aatcggcgca ggtcgcagcc
     3901 tggaaagccg ccagcaggcg ggtgaaatgc tgtttgaact gattaaaacg cacttcgccg
     3961 ccctgatgga gagccgcctg ctggcgttgt cgtttgagat tgaagagctg catccgacgc
     4021 tgaattttaa acaaaacaac gtgcacgcat tgtttaagta gcgcgcagat tgcccggtgg
     4081 cgctgcgctt accgggccta caaaacccca aaccgtacac cgtaggccgg ataaggcgca
     4141 gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc acaccctgat
     4201 cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga
     4261 ttacccggag atcaccatcg aagacgctta cgcggtgcag cgtgaatggg ttcgcctgaa
     4321 aatcgccgaa ggtcgcacgc tgaaaggcca caaaatcggc ctgacctcga aagcgatgca
     4381 ggcaagctcg cagatcagcg aaccggatta cggcgcgctg ctggatgaca tgttcttcca
     4441 cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag tggagctggc
     4501 tttcgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg tctacaacgc
     4561 cacggactac gtgatcccgg cgctggagct gatcgacgct cgctgccaca acatcgatcc
     4621 ggaaacccag cgcccgcgca aagttttcga caccatttct gataacgccg ccaacgccgg
     4681 ggtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt ggatctccgc
     4741 cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggcg tgctgaatca
     4801 tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg tacaactgga
     4861 agccgggcaa atcattctcg gtggttcgtt cacccgcccg gttccggcgc gtaagggcga
     4921 caccttccac gtcgattacg gcaacatggg ttccattagc tgccgctttg tttaaggaga
     4981 gaacgatgga aaacagcttt aaagcggcgc tgaaagcagg ccgtccgcag atcggattat
     5041 ggctggggct gagtagcagc tacagcgcag agttactggc cggagcagga ttcgactggt
     5101 tgttgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcact cagctacagg
     5161 cgattgcgcc ctaccccagc cagccggtag tacgtccgtc gtggaacgat ccggtgcaaa
     5221 tcaaacaact gctggacgtc ggcacacaaa ccttgctggt gccgatggta caaaacgctg
     5281 acgaagcccg tgaagcggta cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg
     5341 gcagtgcgct ggcccgagcc tcgcgctgga atcgcattcc tgattacctg caaaaagcca
     5401 acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc
     5461 agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg
     5521 atatgggtta tgccggtaat cctcagcacc cggaagtaca ggccgcgatt gagcaggcga
     5581 tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat gagcaactgg
     5641 caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc
     5701 tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtgc gcaggctaca gcgattaagc
     5761 ccgacgtgta ttaatgccgg atgcgctgcg cttatccggc ctacactcgc accaaaccgt
     5821 aggccggata aggcgtttac gccgcatccg gcaaaaagcc gtaccaaatc gcggaccgga
     5881 taaggcgttt gtgcgcatcc gccataaacc ttgtcgtacc ctacaaaaat cccattagag
     5941 gaagtaaaat gagcgacacc tcacctgcca taccggagag tatcgatccg gcgaatcagc
     6001 ataaagcgct gactgccgga caacaggcgg ttattaagaa gctatttcgc cgcctgatcg
     6061 tctttctgtt cgtgctgttt atcttctcgt tccttgatcg catcaacatc ggctttgccg
     6121 gactcacgat gggacgcgac ctcggtctga gcgccaccat gtttggcctc gctaccacct
     6181 tgttctacgc cgcttacgtc atcttcggca ttcccagcaa cattatgctg agtattgtcg
     6241 gtgcacggcg ctggatcgcc accatcatgg tgctttgggg catcgcctct actgccacca
     6301 tgtttgccac tggccccacc agcttatacg tactgcgtat actggttggc attaccgaag
     6361 ccggctttct gcctggcatt ctgctgtatt taaccttctg gtttccggcc tatttccgcg
     6421 cccgtgccaa cgccttgttt atggtagcaa tgccggtaac gacagcgttg ggatcgatcg
     6481 tttccggcta cattttgtcg ctggatggcg taatggcatt aaaaggctgg cagtggctgt
     6541 ttttgctgga aggcttcccg tcggtattac tcggcgtcat ggtgtggttc tggcttgatg
     6601 actcaccgga caaagctaag tggctgacga aagaagacaa aaaatgcctg caagagatga
     6661 tggataacga tcgtctgacg ctggttcagc cagagggagc catcagccac cacgccatgc
     6721 aacaacgcag tatgtggcgg gagatcttca ctccggtggt gatgatgtat accctggcgt
     6781 atttctgcct gaccaacaca cttagtgcga tcagcatctg gacaccgcag atcctgcaaa
     6841 gctttaatca gggcagcagt aatatcacca tcggcctgct ggccgccgta ccgcagattt
     6901 gtaccattct cgggatgatc tactggagcc gtcactcaga tcgccgccag gaacgaaggc
     6961 atcacaccgc ccttccttat ttgttcgctg ccgctggttg gttactggct tcggcaactg
     7021 atcacaacat gatccagatg ctggggatca ttatggcttc gaccggatca ttcagcgcaa
     7081 tggcgatttt ctggacaaca ccggatcagt ccatcagcct gcgggcacga gcgatcggta
     7141 ttgcggtgat caacgccact ggcaacattg gttcagcgtt aagtccgttt atgatcggct
     7201 ggttgaaaga tctgaccggc agctttaaca gtggattgtg gtttgttgcc acgctgctgg
     7261 tgattggtgc ggggattatc tgggcgattc caatgcagtc ctcccgtccg cgagcgaccc
     7321 cgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat tgatatcagc aaagagtacg
     7381 atgaaagcct gggcacggac gatgtgcatt atcagtcgtt cgcccgcatg gcggcctttt
     7441 ttggccgcca tatgctgcca catcgccacg aacagtactt tcagatgcat ttcctcaata
     7501 gcggacagat tgagctacag cttgacgatc atcgctactc ggtggaagcg cccctgtttg
     7561 tcctgacgcc gccgtcagta cctcatgcgt ttattacgga gtctgatgct gacggtcatg
     7621 tattgacggt acgggaagat ctgatctggc ccctgctgga agttctttat ccgggcactc
     7681 gggaaacctt cggcctgccg gggatttgcc tgtcactggc agataaaccc gacgaactgg
     7741 cggcgctgga acactattgg caactgatag agcgggaatc ggtagaacaa ctgcctggtc
     7801 gggaacacac cctgacgtta ctggcacagg cagtgttcac cctactgctg cgtaacgcaa
     7861 aactcgacga ccatgccgcc agcggaatgc gcggagaatt aaaactgttc cagcgttttc
     7921 atatgctgat tgaaagccat tttcatcagc actggacagt accggattac gctaacgaat
     7981 tgcatatcac cgaatcacgc ctcactgaca tctgccgccg ctttgccaac cgtccgccaa
     8041 aacggttgat tttcgacagg cagctgcgag aagccaagcg gctgctgctg ttttctgata
     8101 acgccgtgaa caatattgcc tggcaactcg gttttaagga tccggcttat tttgcgcgct
     8161 tttttaatcg cttagtcggt tgctcgccca gtgcttatcg cgccaaaaaa gtacctgtga
     8221 cgtgaaaatc ccttagcctt aacgggaaac caggcaccac ctgctattcc cctttctccg
     8281 cctggagcaa ggggatttag taacgttaaa aatcgctaaa agcgacctcg atcacaaaac
     8341 cgtacccgat ctgaaaagta ccagccattg tcccaaaagt gtcttcccgt aatcgcccct
     8401 gcaagcttca attgaataac aaaaacaaca cacaaaatta acaatcgtat tccgattaat
     8461 actgtagagg tcgacatgaa accagaagat ttccgcgcca gtacccaacg tccgttcacc
     8521 ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga tctatatcta tggcgagcga
     8581 gtgaaagacg tcaccactca tccggcattt cgtaatgcgg cagcgtctgt tgcccaactg
     8641 tacgacgcgc tacacaaacc ggagatgcag gactctctgt gctggaacac cgacaccggt
     8701 agcggcggct atacccataa attcttccgc gtggcgaaaa gtgccgacga cctgcgccag
     8761 caacgcgacg ccatcgctga gtggtcacgc ctgagctatg gctggatggg ccgtacccca
     8821 gactacaaag ccgctttcgg ttgcgcactg ggcgcgaatc cgggctttta cggtcagttc
     8881 gagcagaacg cccgtaactg gtatacccgt attcaggaaa ctggcctcta ctttaaccac
     8941 gcgattgtta acccaccgat cgatcgtcat ttgccgaccg ataaagtgaa agacgtttac
     9001 atcaagctgg aaaaagagac tgacgccggg attatcgtca gcggtgcgaa agtggttgcc
     9061 accaactcgg cgctgactca ctacaacatg attggcttcg gctcggcaca agtgatgggc
     9121 gaaaacccgg acttcgcact gatgttcgtt gcgccaatgg atgccgatgg cgtgaaatta
     9181 atctcccgcg cctcttatga gatggtcgcg ggtgctaccg gctcgccgta cgactacccg
     9241 ctctccagcc gctttgatga gaatgatgcg attctggtga tggataacgt gctgatccca
     9301 tgggaaaacg tactgatcta ccgcgatttc gatcgctgcc gtcgctggac gatggaaggc
     9361 ggttttgccc gtatgtatcc gctgcaagcc tgtgtgcgcc tggcagtgaa actagacttc
     9421 attacggcac tgctgaaaaa atcactcgaa tgtaccggca ccctggagtt ccgtggtgtg
     9481 caggccgatc tcggtgaagt ggtggcgtgg cgcaacacct tctgggcatt gagtgactcg
     9541 atgtgttcag aagcgacacc atgggtcaac ggtgcttatt taccggatca tgccgcactg
     9601 caaacctatc gcgtactggc accaatggcc tacgcgaaga tcaaaaacat tatcgaacgc
     9661 aacgttacca gtggcctgat ctatctccct tccagtgccc gtgacctgaa caatccgcag
     9721 atcgaccagt atctggcgaa gtatgtgcgc ggttcgaacg gtatggatca cgtccagcgc
     9781 atcaagatcc tcaaactgat gtgggacgcc attggcagcg agtttggtgg tcgtcacgaa
     9841 ctgtatgaaa tcaactactc cggtagccag gatgagattc gcctgcagtg tctgcgccag
     9901 gcacaaagct ccggcaatat ggacaagatg atggcgatgg ttgatcgctg cctgtcggaa
     9961 tacgaccaga acggctggac tgtgccgcac ctgcacaaca acgacgatat caacatgctg
    10021 gataagctgc tgaaataacg cagcaggagg ttaagatgca attagatgaa caacgcctgc
    10081 gctttcgtga cgcaatggcc agcctgtcgg cagcggtaaa tattatcacc accgagggcg
    10141 acgccggaca atgcggaatt acggcaacgg ccgtctgctc gatcacggat acaccaccgt
    10201 cgctgatggt gtgcattaac gccaacagtg cgatgaaccc ggtttttcag ggcaacggta
    10261 agttgtgcgt caacgtcctc aatcatgagc aggagctgat ggcccgccac ttcgcgggca
    10321 tgacaggcat ggcgatggaa gagcgtttta gcctctcatg ctggcaaaaa ggtccgctgg
    10381 cacagccggt gctgaaaggt tcgctggcca gtcttgaagg tgagatccgc gatgtgcagg
    10441 caattggcac acatctggtg tatctggtgg agattaaaaa catcatcctc agtgcagaag
    10501 gtcacggact tatctacttt aaacgccgtt tccatccggt gatgctggaa atggaagctg
    10561 cgatttaagc tccagcccct tcacgcattc gtcgaagggg catttttatt gtgatctcaa
    10621 tcacatatcc acccgatgaa taattcttac ccaaccgctc accgccattc ctgaccgctt
    10681 acatccctaa aataaccact cagttattta ccttacttta cgcgtgcgta actctggcaa
    10741 catcactgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc tagaacggct
    10801 tcggcctatg attaacaaat tactctatgg attttggttt gcaggaaggc agcaagtgag
    10861 tgaatccccg ggagcttata acagtaagtg acaggggtga acgaacgcag ctgccgcacc
    10921 tgtaagccaa aagacgacga gtaaaatgcc aggttttact atggatacga aaaaactatt
    10981 caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg cggtagttgc
    11041 attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta
    11101 tctggtggcg tatcgctact acagtctgta tatcgcccag aaggtgatga aactcgaccc
    11161 cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc cgaccaaccg
    11221 ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc tggttggtcc
    11281 ggttctcgcc gcgcagatgg gctacctgcc cggcacgctg tggctgctgg cgggggtagt
    11341 gctggccggt gcggtacagg actttatggt gctgtttatc tcctctcgcc gtaacggcgc
    11401 atctctcggt gagatgatca aagaagagat gggaccagta ccggggacta tcgcgctgtt
    11461 tggctgtttc ttaatcatga tcatcatcct tgccgtcctg gcgctgattg tggttaaagc
    11521 cctggcagaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga ttgcgctgtt
    11581 tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct ctgtaatcgg
    11641 tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg atccgtactg
    11701 gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg gctatgcgtt
    11761 tgtttccgca ctgctgccgg tgtggctgat cctcgcaccg cgcgactatc tggcaacctt
    11821 cctgaaaatc ggcgttatcg tcggcctggc gttgggtatc gtggtgctga acccggaact
    11881 gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga aaggcgctct
    11941 gttcccgttc ctgttcatca ccatcgcctg tggtgcggta tctggcttcc acgcgctgat
    12001 ctcttccggt acgacgccga aactgctggc taacgaaacc gacgcgcgtt tcattggcta
    12061 cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgcag cgtccatcat
    12121 cgaaccgggt ctttacttcg cgatgaacac cccgcctgcg ggccttggca tcaccatgcc
    12181 taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc tgaaagacgt
    12241 taccgcacac gcggcggcga ccgtcagctc ctggggcttc gtgatttcgc cagagcagat
    12301 cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcgcc
    12361 gacactggcg gtaggtatcg cgcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg
    12421 cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg cgctggatgc
    12481 gggtacccgt tctggccgct ttatgctgca ggacctgctg ggtaacttca tcccgttcct
    12541 gaaaaaaacc gactctctgg ttgctggtat catcggtact gcgggctgtg tgggtctgtg
    12601 gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc
    12661 gctgttcggt atctccaacc agatgctggc agccgtagcg ctggtactgg gcaccgttgt
    12721 gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg ctgtatggct
    12781 gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc cgcagatgga
    12841 aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta ctgacctgac
    12901 ggcacagcag attgccaaca tgaaccacat cgttgtgaac aactacacca acgcgggcct
    12961 gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca aaacctggct
    13021 tgcggtgcgt aacagcgaca aacgtactga caaagaaaca ccgtacgttc caatcccgga
    13081 aggcggcgtg aagatctctt cgcaccacta accggttgcc ggatgcgttg cgcgtatccg
    13141 gcctacaaag tgtctatcgt aggccggata aagcgtttac gccgcatccg gcaaatccct
    13201 agcccggacc ttccgggctt tacgcatatc tggaaggcaa aatgtttggt aacttaggac
    13261 aggcaaaaaa atatctcggc caggcggcga agatgttgat tggcattcca gactacgaca
    13321 actacgtcga gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat
    13381 tcttccgcga acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt
    13441 gttaatggag agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg
    13501 ggcaaaacca ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg
    13561 attgaaaacg aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg
    13621 cagatcaaaa cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaagac
    13681 gcactactcg acctgctgga taatctcgac aagggcaata ttcagttcga ccgtctggtc
    13741 attgaatgca ccggcatggc cgatcccggt ccgattattc agaccttttt ctcccatgag
    13801 attttatgcc agcgttacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc
    13861 gatgagcaga tgaaccagtt caccatcgcc cagtcccagg ttggctacgc cgaccgtatt
    13921 ctgctgacca aaaccgacgt cgcaggcgaa gcagaaaaac tgcgtgaacg cctggcgcgc
    13981 atcaacgccc gtgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgcta
    14041 ttcaacacca acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgcttccac
    14101 tttatcgcgg ataaacaaaa cgatatttcg tcgattgtgg tggaactgga ttacccggta
    14161 gatatcagcg aagtttcccg ggtgatggaa aacctgctgc tggaatcggc agataaactg
    14221 ctgcgttaca aagggatgct gtggattgac ggcgaaccta accgcctgct attccagggc
    14281 gtccagcgcc tctacagcgc cgactgggac cgcccgtggg gcgatgaaaa accgcatagc
    14341 acgatggtgt ttatcgggat tcagctgccg gaggaagaga ttcgggcggc gtttgcgggg
    14401 ttgaggaagt aatcgtgaaa ggttgagtgt aaagcagctc aatgccccat ttagcagtga
    14461 ggggggaaac agaacgacgt gctcccctcc gctaagtttt attgcgcgtg aatacctgga
    14521 tctggcaaaa aattgccgcg tttggcgtca gaccatgctt cgctaccaga agcaggaatg
    14581 tcgctgtaat caatgtcttc gtcggattta ttaaccaaag ccatgagttc agcttcttgc
    14641 tgcacatcaa gtgtgtagag tgtgccccgt ttatgtttaa ccatgctcat ttatgaccag
    14701 aacctttacg atttaattat aaagatgact tatttcagcc atccaggcac gttatattct
    14761 gagacattct caatggaata tgcatctctc ctgtcagaac ctttcttaca cttcaactca
    14821 ttgacccccc atttaccctc tctattggaa taaaatacag tatatttatc ggtattaaac
    14881 ccgaaatatc tttcatccgg acgtgaaata ttcaactctc cacatttaac ctgattacgc
    14941 cattcaaaat ctatggtgct ttttaaaacg tagtacgaaa taccttctac attttttgaa
    15001 gcaaaagcca ctaaaatagt agatacgata aatgctccgg
//
LOCUS       DAZFDP010000122        14954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFDP010000122 DAZFDP010000000
VERSION     DAZFDP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..588
                     /locus_tag="ACHJ1G_003720"
     CDS             70..588
                     /locus_tag="ACHJ1G_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4765498.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(633..2678)
                     /gene="dcp"
                     /locus_tag="ACHJ1G_003721"
     CDS             complement(633..2678)
                     /gene="dcp"
                     /locus_tag="ACHJ1G_003721"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4765499.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2815..3561
                     /gene="ydfG"
                     /locus_tag="ACHJ1G_003722"
     CDS             2815..3561
                     /gene="ydfG"
                     /locus_tag="ACHJ1G_003722"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4765500.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3650..4336
                     /gene="rspR"
                     /locus_tag="ACHJ1G_003723"
                     /gene_synonym="ydfH"
     CDS             3650..4336
                     /gene="rspR"
                     /locus_tag="ACHJ1G_003723"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4765501.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4514..4717
                     /gene="ydfZ"
                     /locus_tag="ACHJ1G_003724"
     CDS             4514..4717
                     /gene="ydfZ"
                     /locus_tag="ACHJ1G_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4765502.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4753..6213)
                     /locus_tag="ACHJ1G_003725"
     CDS             complement(4753..6213)
                     /locus_tag="ACHJ1G_003725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4765503.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6302..7585)
                     /gene="ydfJ"
                     /locus_tag="ACHJ1G_003726"
                     /pseudo
     CDS             complement(6302..7585)
                     /gene="ydfJ"
                     /locus_tag="ACHJ1G_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            8188..8301
                     /locus_tag="ACHJ1G_003727"
     CDS             8188..8301
                     /locus_tag="ACHJ1G_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO4765504.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            8370..8603
                     /gene="ydfK"
                     /locus_tag="ACHJ1G_003728"
     CDS             8370..8603
                     /gene="ydfK"
                     /locus_tag="ACHJ1G_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO4765505.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            8920..9510
                     /locus_tag="ACHJ1G_003729"
     CDS             8920..9510
                     /locus_tag="ACHJ1G_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4765506.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(9608..10183)
                     /locus_tag="ACHJ1G_003730"
     CDS             complement(9608..10183)
                     /locus_tag="ACHJ1G_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4765507.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(10183..13143)
                     /locus_tag="ACHJ1G_003731"
     CDS             complement(10183..13143)
                     /locus_tag="ACHJ1G_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4765508.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSAMAAGNSVKAAKT
                     SETNAKSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGVVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTG
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTLTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            complement(13208..13807)
                     /locus_tag="ACHJ1G_003732"
     CDS             complement(13208..13807)
                     /locus_tag="ACHJ1G_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4765509.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            complement(13878..>14954)
                     /locus_tag="ACHJ1G_003733"
     CDS             complement(13878..>14954)
                     /locus_tag="ACHJ1G_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4765510.1"
                     /translation="KPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGQIT
                     ESHLGKELLEKVELTEDNASKLEEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIG
                     LSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTIT
                     SGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDF
                     VKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGN
                     NPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGN
                     QGAGNITDCTVIVTKKAASGISIR"
BASE COUNT         3767 a         3865 c         3425 g         3897 t
ORIGIN      
        1 attattatat ttgacgagca tacagaaaaa gcaaatcacc tttacatata atagcgtgga
       61 caaaaaacag tgaacattaa taaagataaa attgtacaac tcgcagatac cgacactatt
      121 gaaaacctga catccgcgtt gagtcaaaga cttatcgcgg atcaattacg cttaactacc
      181 gccgaatcat gcaccggcgg taagttggct agcgccctgt gtgcagctga agatacaccc
      241 aaattttacg gtgcaggctt tgttactttc accgatcagg caaagatgaa aatcctcagc
      301 gtaagccagc aatctcttga acgatattct gcggtgagtg agaaagtggc agcagaaatg
      361 gcaaccggag ccatagagcg tgcggatgct gatgtaagta ttgccattac cggctacggc
      421 ggaccggagg gcggtgaaga tggtacgcca gcgggtaccg tctggttcgc gtggcatatt
      481 aaaggccaga cctatactgc ggttatgcat tttgctggcg actgcgaaac ggtattagct
      541 ttagcggtga ggtttgccct cgcccagctg ctgcaattac tgctataacc aggctggcct
      601 ggcgatatct ccaggacagc cattggtggt gtttatatgt tcaagccacg atgttgcagc
      661 atcggcataa tctgaggagc cttaccgcgc cattgtcgat acaggcgttc cagatcttcg
      721 ctgttacctc tggaaaggat cgcctcgcga aaacgcagcc cattttcacg cgttaatccg
      781 ccctgctcaa caaaccactg ataaccatca tcggccaaca tttgtgtcca cagataagcg
      841 taataacctg cggcatatcc gccaccaaaa atatgggcga aataactgct gcgatagcgt
      901 ggcggtatag caggaagatc catattttcc gccaccagcg cccgcaattc aaaatcatcg
      961 acatcctgca ttgcttcgtt ttcttccagg caatgccagc gcatatcgag aagtgcggcg
     1021 ctaagcagtt cgctcatctc ataccctttg ttgaacaggc tggcattacg cattttctgt
     1081 tgcagttcgt caggcattgc tgccccgctc tgataatgcc gggcgtagcg agcgaatacc
     1141 tgcggatgcg ttgcccagtg ttcgttgatt tgcgacggaa attcgacaaa atcacgcggc
     1201 gtgttggtgc cggaaagcgt ggcataacgc tggcgggcaa aaaggccgtg cagcgtatga
     1261 ccaaattcat ggaataaggt tatgacatca tcccagagta acaacgcagg ctcaccggca
     1321 gcgggtttct gataattgca aacgttataa attaccgaat gcgtttcatt aagcgttgat
     1381 tgctcaacaa aattgcccat ccatgcaccg ccgctttttg aatcacgggc gaagaaatca
     1441 ccgtaaaata acgccagtcc cacgccatta tgatcaaaaa tttcccacac acgaacgtca
     1501 ggatggtaga caggaatatc aaaacgttcg acaaacttaa taccgaagag ctgattcgcg
     1561 gtccagaata caccttcatt caacaccgtg tttaattcaa aatatggctt gagctgcgcc
     1621 tcatcaagat caaatttctc ccgccgtacc tgttcggcat aaaatgccca gtcccacggc
     1681 tgcgcgctaa acccgccctg ctgcttatca ataaccgcct gtatggaggc taattcatcg
     1741 ctcgcacgtt gacgcgccgc tggaacaatt tcccgcataa agttgagcgc tgcttcaggt
     1801 gtttttgcca tctgatcggc gattttccat gcggcataat gaggaaaacc aagtaatgtc
     1861 gcctgttgtg cacggatctc caccagacgt tgaatgatag cgcgggtatc attgccatca
     1921 tttttttccg ctcgcgtcca gcccgcaata aacagttttt cacgcgtcgc acgatcgcgc
     1981 atttcggcaa gcgccggttg ttgggtggta ttcagcagcg gaatcagcca tttgttatcc
     2041 agacctttct cgcgagccgc ctctgccgcc agcgcaatct cttgctcact cattcctgcc
     2101 agctgcgcga tatcgttcac aaccagaccg ccggatttat ttgctgccag taaccgctgg
     2161 ttaaactgac tggtcagtgt cgcagcttct gtattcagta cttttaattt tgctttatca
     2221 gcttgcgcaa gtttggctcc ggcaaggaca aaacgttgat gaatcacctc caccaggcgg
     2281 atggattcac tatcaagccc cagggattca cggcgctgcc agacagcatc tacccgcgcg
     2341 aataattcac cgttcagata gatatcatta gccagttccg ccagttcagc ggaaaactgt
     2401 tcatcaagac gctgtaattc atcattggta tgcgccgcag tcatcgcaaa aaagacgctg
     2461 gtaacgcggg taagtaattc tccgctttgt tccagtgcca gaatagtatt gttgaaatca
     2521 ggcgtttgcg ggttaagcgc gatggcagca atttctgccc gcttttgctg cattccctca
     2581 tcgaatgccg ggcgatagtg atgattggca atttgatcaa aatgaggagc cagatacggc
     2641 agtgtgcttt gcacaaggaa aggattcatt gttgtcattt tcttctcctg aacgcgaggt
     2701 gttccatagc gtaggcttac tgataacgga gtgcaatctt gcaaaccagt attacccgct
     2761 cttaagcatc acgtgctatg ttattgacac acaaaagcgt tgaggaacag tgagatgatc
     2821 gttttagtaa ctggagcaac ggcaggtttt ggtgaatgca ttactcgtcg ttttattcaa
     2881 caagggcata aagttatcgc cactggccgt cgccaggagc ggttgcagga gttaaaagac
     2941 gaactgggag ataatctgta tatcgcccaa ctggacgttc gcaaccgcgc cgctattgaa
     3001 gagatgctgg catcgctccc tgccgagtgg tgcaatattg atatcctggt aaataatgcc
     3061 ggcctggcgt tgggcatgga gcctgcgcat aaagccagcg ttgaagactg ggaaacgatg
     3121 attgatacca acaacaaagg cctggtatat atgacgcgcg ccgtcttacc gggtatggtt
     3181 gaacgtaatc atggtcatat tattaacatt ggctcaacgg caggtagctg gccttatgcc
     3241 ggtggtaacg tttacggtgc gacgaaagcg tttgttcgtc agtttagcct gaatctgcgt
     3301 acggacctgc atggtacggc ggtgcgcgtc accgacatcg aaccgggtct ggtgggtggc
     3361 accgagtttt ccaatgtccg ctttaaaggc gatgacggta aagcggaaaa aacctatcaa
     3421 aataccgttg cattgacgcc agaagatgtc agcgaagccg tctggtgggt gtcaacgctg
     3481 cctgctcacg tcaatatcaa taccctggaa atgatgccgg ttacccaaag ctatgccgga
     3541 ctgaatgtcc accgtcagta atttttatac ccggcgtaac tgccgggtta ttgcttgtca
     3601 caaaaaagtg gtagactcat gcagttaact cactcacaag caagaacgaa tgaccgtcga
     3661 aacgcaactt aatcccacac agcctgtcaa tcagcagatt tatcgtattc ttcgtcgcga
     3721 cattgtccat tgcctgattg caccaggcac accgttgtcg gaaaaagaag tttctgttcg
     3781 tttcaatgtg tcacgccagc cggttcgtga agcctttatt aaactggcgg aaaacggcct
     3841 gattcaaatt cgtccgcagc gtggcagcta cgtcaacaaa atttccatgg cccaggtgcg
     3901 aaacggcagt tttatccgtc aggccattga gtgcgcggtg gcgcgtcggg cggcgagcat
     3961 gattaccgaa agccagtgct accaactgga acaaaatctt caccagcaac gcattgccat
     4021 tgagcgcaag caactggatg atttttttga acttgatgac aacttccatc aactcctgac
     4081 gcagattgcc gactgtcaac tggcgtggga taccattgag aacctgaaag cgaccgttga
     4141 tcgcgtgcgc tatatgagtt tcgaccatgt ttctccacca gaaatgctgt tacgccagca
     4201 tcttgatatt ttctctgccc tgcaaaaacg tgatggcgat gcggtagaac gtgcaatgac
     4261 gcaacatttg caggaaatca gcgaatccgt gcgccagatc cgccaggaaa acagcgactg
     4321 gtttagcgaa gagtaattca tttactctca tcccatccgg gatgagaggc ttttcccccc
     4381 gccttacggc tcatgcatgc atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt
     4441 tcactcgaca ggagtattta tattgcgccc gttacgtggg cttcgactgt aaatcagaaa
     4501 ggagaaaaca cctatgacga cctacgatcg taaccgtaac gcaatcacca ctggcagccg
     4561 tgttatggtt agcggcaccg gtcacactgg caagatcctg tcgattgata ctgaaggtct
     4621 gaccgctgag caaatccgcc gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact
     4681 ggcacctctg gacctgattc gtctcggcat gaactaagtg tgtgaatgcc gccgatggcg
     4741 gcattgcttt ttttacttca cggaatattt cgccacggtc gctttcgcgc catgcgctaa
     4801 taaagacaag tacgcttccg tcacttttgc agtaaacaag ctattgtctg gcaaatcatc
     4861 accaaagatt gccttaatcg ccagcaatga ctggacgcgc gctttccctt cggcactact
     4921 ttgtacagcc ttctgaataa caggtaacag tgggtcactg atttctatca gatttccctg
     4981 ttcatcaaca ccaccgacat aacgcatcca acccgcgacg cccagcgcca gcagatcgaa
     5041 cttgctgtca tgcgccagat gccagcgaac agaatccaac atccgctgtg gcaatttctg
     5101 gctaccatcc atcgcaatct gccaggttcg atgacgtaac gccgggttgc tatagcgtgc
     5161 aattaatcgg ttagcgtaat cttgcaaatc aacgccctgc actttcagcg tcggcgcttg
     5221 ttcctgcaac atcaagccat acgccgcata acgataatgt tcatcttcca tgcagtcatt
     5281 aatgtgctga tatccagcaa gataacccaa atacgccagg aatgaatgac tgccgttgag
     5341 catgcgcaac ttcatctctt cataaggcag cacatcacta accagttcgg ctcccgcttt
     5401 ttcccattct ggacgtccgg caacaaagtt atcttctatt acccactggc ggaaaggttc
     5461 acaggcaacg cccgcaggat cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc
     5521 ctctgtcact gcgggcacaa tacggtccac cattgttgat gggaaagtca cgttatcttc
     5581 gatccattgt gccagtttta catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat
     5641 aacatgaccg ttttctggca tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc
     5701 cgctttacgg cgggccagcg cctcaacaat cactcctgtt gctgttttcg gctggtgggg
     5761 attttgcacg tcggcagcga ccatcgggtg atcgagcatt aactgtccga tcgccggaga
     5821 gtggaaatac cctttttcgg tgattgtcag agagacaatc gcgatttgcg gttcacacat
     5881 cgcagccaac acggtttcta agccatccat ctgtacgtgc aaggcttttt taacgacgcc
     5941 aacgacgcga gccgtccacg catcggccga catttccgca acggtataaa gattatcttg
     6001 ctgttgtaaa tcggcaattt gctgttcgcc gccgattaag ttgacctcat aatatcccca
     6061 gtcactgaaa tgttctgtag caagaatatc ggcatacacg ccctgatgcg cacggtgaaa
     6121 tgcaccaaag cctaaatgaa taattcttgg ggccaggtta ttacgatcat aaacagggag
     6181 tgtcgctttt gctgataaca aattatttcc cataacaatt ccttaaatat aaatatggca
     6241 agctatatgt tttgttatat gaataaaaat cccctctccg gtaagagaag ggattgagga
     6301 tttacagact tctggaaggt tgcgcagctc ttacaacacg cggttgatct tccgcagcgt
     6361 cttccagcgc acttaaatca cggtctttca cctctggcat tttcagcgca gagattaaac
     6421 caattactga atatgccatg atcataatgg cgatcggata ccaggattcc gtcatggtgc
     6481 agaaaatacc cgccaggata ggaccaaaac cggaagcgat aagaccacca atttctttag
     6541 aaatagccat ccgggtaaag cggtttttac agccgaacat ttctgccatg gtaatgtttt
     6601 ccagagcaaa taatcccagc accgcacagt tatgaatcac aatcagtgca accataatgg
     6661 tgctcggggc atagctttta tctacaatga tagaaagcat tggccatgcc agcacaatcg
     6721 cggaggtatt cataataata tacgggatcc ggcgaccaat tttatcggat aaccaaccaa
     6781 ggaacggaat ggtcataaag ccgagaatcg aactaatcat caatgcatct gttggaattg
     6841 ctttgttaaa caataacgtc tgcactaaat agcctgcaag gaaagtctga attaacccgg
     6901 agttacccgc ctgaccaaaa cgcagccctg ttgccagcca gaaggatttg ctctggaaca
     6961 tgctaccagc aggtgcaggt tttgctgtgg gttggttgct gtcgttaacc ttctcaaaga
     7021 ccgggctttc tttcagattc atacgcaacc agatagcaaa gaccatcacg acaacgctcg
     7081 ccaggaacgg tatacgccat ccccacgcaa gcagttcctc tttactgaga atgaagaaca
     7141 taaaggccca gattgccgtt gcgctcaagg ttccgcagtt agttcccata gctacaaatg
     7201 aggagataat tccgcgctta ccttttggcg catattccgc cagcatcgta ccggcaccgg
     7261 aaatttccgc acctgcaccc aacccctgaa taatacgcag cgtcaccagc aagatggggg
     7321 caaaaacacc aatctgtgca taggtcggta acacaccaat taaggtggta cagatcccca
     7381 tcatggtgat ggtaataaag agcacttttt tacgccctat tctgtcgccc attttgccga
     7441 aaataaatgc tccgacaata cgcgccacat aacctgcacc gtaggttccc attgccaaaa
     7501 ttaacgccat tgccgttgat gattcagaaa aaaatatttc atgaaacact aacgctgcgc
     7561 cgagcgaata taactggaaa tccataattc acaggtgttt tttcccatcc tgtggtttcc
     7621 ttggcgtttt ctaggttttt tcagatagtt gcattttttt aaaagcatcc taagttcgat
     7681 ctcagtgtct atctggggcc tatttctgtc ccatatatgc cccaaaaaac tccccaacag
     7741 ataagtagtt ttttcatgga tttacgcgta aaatcaataa cggctggaaa tcattcaata
     7801 ctcgcactat cgaaaaattt accagccaac cgcagcacgt tcttgcataa ggtgtgtctg
     7861 cggtttttca actattcaga tacatcactc ccatcacatt cattcctcca catcaaaggc
     7921 atataggcta tatcaccttg atatttttct ttttcagata aaaactgtta tctatgtata
     7981 cttttaaacc caatccgtgt agagtctctg cataagatag tttgcagttg ccgcttcagc
     8041 ttgcgccata aaccgcctga tttttgctgc cacctgttag cattcctgta tacctgaaac
     8101 gacaatgttt atctacgaac tttaagaaca cccaagataa aaattgtcaa ctatatcata
     8161 tataacacat tactaattcg aggctatatg aacagcatac tgataatcac ttctctcctt
     8221 atcatattca gcatttttag tcatgcccta ataaaattag ggattggcat atccaataac
     8281 ccagacaaaa ccgatgtata agtcaacata tcctgaatca gacatacaat atcgcaatga
     8341 aaatcaataa tattttaagg aatatcttca tgaaatcaaa agacacccta aagtggttcc
     8401 ctgcgcagct tcctgaagta agaattatcc taggggatgc tgtagtggaa gtagcaaaac
     8461 agggaagacc tatcaatacc agaacattgc ttgattacat tgaaggaaac ataaagaaaa
     8521 aatcatggct ggataacaaa gaattattac aaacagcgat atcagttctt aaagacaacc
     8581 aaaatttaaa tggtaaaatg taatataata aacttacttt tttatcattt ttccacttta
     8641 acaacatttt gctccacttt tccacgacca aacaacttga aatctggtta aaataacacg
     8701 caacactatt cttcttcctt gagtccgccc ggaactcgaa aaacaaaccg agttaaagcc
     8761 atttttcaca aaatcgattt tgggtctcac caaaattacg gggttgcata cgcattcgtt
     8821 tattttcgaa cgtgtacata caaatatgca caaaaataat caaaattatt ttctgagatg
     8881 cattatgata tgaacaccaa tttcgtatag agtctcacta tgtctcgaat ttttgcttac
     8941 tgtcggatat caacgctgga tcagaccatc gaaaatcaac gccgggaaat cgaaagtgca
     9001 ggttttaaaa tcaaacctca gcaaataatc gaagaacaca ttagcggctc agcagcaacc
     9061 agtgagcgtc ctggttttaa ccggttgctt gctcgcctga aatgtggtga tcaattgatt
     9121 gtgacaaaac tggatcgcct tggttgtaat gcaatggata tcaggaaaac agtggaacaa
     9181 ctgaccgaaa caggtatcag agtgcattgc ttagcattgg ggggcattga cctgaccagt
     9241 ccaacaggaa aaatgatgat gcacgtaatt tcagcagtcg ctgaatttga acgagacctt
     9301 ttacttgaac gcactcattc cgggatagta agagcccgcg gcgcagggaa acgttttggt
     9361 cgaccacctg tgttaaatga agaacagaaa caggtggtat tcgaacgaat taagtcaggt
     9421 gtaagtataa gtgccattgc ccgggaattc aaaacctcgc ggcaaaccat tttaagagcc
     9481 aaagcaaaac ttcagacacc tgacatataa aaaataatct cggtgtgaga tgctttacgt
     9541 cttccaagcc cccttccttg ccgtaaatgg aaagatacat ctaattatag aatttatatg
     9601 ttttacccta cggcagtgct ggccattcaa tatcctgtgc agttgacgta tcaacacggt
     9661 tcagcaacac ccgatacttt ttccaggctt ccagcaatga ggtttcttcc tccgttgcga
     9721 tttccagatc tacagcatcc tgaagtggcg caatatgctc actggctacc tgcatcaggc
     9781 tgttttttgt ttcttccgcc tctcggatcc ggaacagttt ttctgcttct gcatctttca
     9841 cccaggctgt gccgttccac ttctgaaact ccccttccgg cgataaccag gtaacatttt
     9901 ccggtaacgg accgagttca gaaataaata acgcgtcccc tgacgctacg tcataaaccg
     9961 ttttaccccg atggtcttca acgagatgcc acgatgcctt atcactgttg aaaacagcca
    10021 caaagccagc aggaatatct ggtggtgcaa tatcggtact gtttgctggc agacctgtat
    10081 gaggcggaat atatgcgtca ccttcaccaa taaattcatt agttccggcc agcagattat
    10141 aaatttttat ggtccgtggt tgttcactca ttctgaatgc cattatgcaa gcctcacaat
    10201 atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt taacggtgat
    10261 ggtgtgtcca tgtgaaccaa tcgcaacgga gtgcgtatga gcaccaatac cgacagtatg
    10321 cgcgtgtgca cctgcagatg ctgctgtgcc ggacagtgaa tggctatgat taccatctgt
    10381 actggtattc gctaaccacc ccgtagacat acctactgag ccttgtacac cccaggtatt
    10441 ttgacctgag cttgtataac catattgata agtatcttta aaaacactgg ggttaaatcg
    10501 acggccatct ctatggctgt gattaccagc tgcattcgtg ctgccactta aactatgggt
    10561 atgcgcacca gtgttattcg tggatttagt gccgtaatca aacgacgatg tggttttcgt
    10621 ccccaaatcc gtactggatg cgctggcgct gtgggtatgc gatttaatgc cgtcctgttc
    10681 ctgagacaat acggcccgac cactggcagg tttgccctta atcgtccagc cacgcatatc
    10741 agggatcacg cctgacggat aagccgctgc aagtttcggg tatgcagatt tgtcaaaagc
    10801 ctgcccctgc atcagggcat agccagacgg aacggtatct gatggccacg ggattggtgc
    10861 gccaggcgga taaaactgct ctgatggcgt atagagtgaa taaactgtac cgtccgttaa
    10921 cccttccggc ttattagcag aatatgctgg tgacgtatga atcgtcacgc tggcattact
    10981 ggtataatcc cattgaatat ttacaccagt cgcataattt ccgattgcaa cgtaaatatc
    11041 gtaagtatca ccagatgtat taacccaggc aaaatttgta aaccctgtca atgtgcgctg
    11101 ccataaagca ccagtaatcc ccttcggatt accattacct gcacgtaaaa caagttcaga
    11161 tatacctgcc tgttgaggtg accccacgtt aaatccagcg ccaccaatca acgtaattga
    11221 aacaacagaa ctcgcctgtg gcatggttac cgttgctaat ttgaaccaac cagcaccacc
    11281 gctgaatgac attgttgttg agttaagcgt accaatatct ttcggcgtca gtgttatatc
    11341 cgctgaaagc gccttaccat tcaccttacg ggcagaaggt acccgaccat tcgcattgtc
    11401 attagctgct ttcactgctt tcggtgtcgc agcaagcgtt tcagatgtgc tgttggttgc
    11461 actactgagc tggacaattc ctttttgtgc tgtcgtagcg tcctgtgcgg tgtatttccc
    11521 gttagccagg tcatacgcgg ccttaacggc ttttggcgtt gccgcctgtg actcggaagt
    11581 gctgttagtc gcactgctga gctgaactat ccctttctgt cctgtcgttg catcctgtgc
    11641 ggtgtatttc ccgttagcca ggtcatacgc ggccttaacg gcttttggcg ttgccgcctg
    11701 tgactcggaa gtgctgttgg tcgcactgct gagctgtact accccctttt tcgtcgtgct
    11761 cgcatcctca agcgccacgg cggatgcaat atcctctgca cgttttgccg ctgtctcagc
    11821 gcgcgttgct gcggattctg ccgtactttt gctctgtgct gccgctgccg cactgccagc
    11881 agcctctgtc gccttcgtgg atgccgtcgt ggcgctgccc ctcgctgctg acgcctgtct
    11941 ggtcgcctca tcttttgaag cagacgcaga tgatgccgat gacgccgccg aactggcgga
    12001 cgatgcggca gccgtttttg aggattctgc gctggtttcc gacgctttcg cgttcgtttc
    12061 ggatgtcttc gctgcggaag cagaactcgc tgctgcgctg gcctgttcag tggcttcgcc
    12121 agccttcgtt gtggctgttg aagcggatga tgcggcgctt tctgccgatt ttccggcggc
    12181 ggtggcactg actgctgcct cctctgcctt aatggacgcc tcacgggccg atgatgcagc
    12241 tgctgtctca gagtctgccg cagctgaggc gctctgttcc gctgccgttt cagaagactt
    12301 agcattcgtc tcggacgttt ttgccgcctt cacggaattt cctgccgcca ttgccgagga
    12361 ggctgcactg ctggcgcttg tggaggcgtt cgtttctgat gattttgccg cctctttgga
    12421 ggccgccgca tctctggctg aagtggcggc ttctgacgct ttcgtcgtcg cagtggatgc
    12481 agaagtggct gctgattttt gtgacgctgc agcattcgtt tctgacgttt tcgcggcacc
    12541 ggcactggtg gccgccgcgc tttttgagga ctctgcagcg gcagcacttt ttgatgcttc
    12601 tgtggccttt gttgatgctg ttcctgcgct ggaagacgct gactgagccg acgtcgcggc
    12661 ctgcccggct gatgtgctgg ctgcacgtgc tgagcctgca gcatcagtcg catgggttgc
    12721 cgcctcacgg gctgatgtgc cggcatcgcc ggctgacttc ttcgcggctg ccgtgttctg
    12781 tgccaccgcg gacgcgttac gcgccacctc ttccaccatc agctcaaaac ggcgcagtgc
    12841 ctccggacgg gcatcatcct ccgtcatggc accgagaaaa tcattcagcg taccgggtcg
    12901 ggaatcttca tacacggtga tggtcccggc atgtgacggc gggaatcctt ccaccaacag
    12961 aataacgctg tactgaccgt actcaacgtc catactgtaa cgcccggctt catccggatt
    13021 ttctgaggcc agcgtgttca ccaccaccgt ggtgctgtta cgttttgctt tcagctggat
    13081 tgtgcagttc tgtaccggtt ttcctgtgcc gtctttcagt acacctgaaa tctttactgc
    13141 catattcacc ccacaaaaaa gcccgcctga accggcgggc tgtcataaca ctgtgttacc
    13201 tggctaatca gaatttataa ccgacaccca cgatgaaact gttggtacgc cagtcaccgc
    13261 tgccggagcc ttcataagcg acgtcaacgg ccacggattc ggtcgggtta aactgcacgc
    13321 cagctcccca cgccagagac gtgttgctgt ggcgaccgtc atcacttccg gtcagcacat
    13381 cgtgcgtttt ccccttgttg tcagttacgc ggatataatc tccggagaaa gtcgaaacac
    13441 ggctgtaagc catacccgcc atcgcatacg cgctgaacca ttcattcacg cgcacagacg
    13501 gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag cgggtatcgc
    13561 tgtaatgcgt tttttgctca tcttcagcgt tggcataact gaatgacgta atcagcccca
    13621 gcgtgtccgt aaactcataa cggtatttca cgttaatccc gttcagatta tcgctgccgg
    13681 gagcgttcgt acgggcatga agataccctg cgctcagtgt ggcctgctgc tcagacgccc
    13741 atgcaggcgc accggatacg gccagacaga tggctgcgga caaaatggct gcacaaactt
    13801 tacgcataat tacctctcgc ttttctgcaa taaaaaaggc gtcattcctg acgcccttta
    13861 ttggggttat aaatatttca acgaatactg atgccggaag cagctttttt ggtcacaatc
    13921 accgtacaat cggtgatatt gcctgccccc tgattgcctt tctggaaaat cttaaactcc
    13981 agagtgacgc taccaccacc actaggcata tcaataactg cactgtaact accgggaatg
    14041 gcccctttag tttctctgga tgcgattaat acgccgtttt tgcgaacttc aaaaccataa
    14101 cccgtgtatc gcgtgcctcc tgggttattt ccgctccccg gatcgtcata cgctataccg
    14161 ttaaaaataa tgggcggaat aataatctgg cggtcaaagt tatgatcatc gctgatggtg
    14221 actgtaaccg taccgtttgg tgtttccgtg ttaccccacg taccgacttt tttcgggaag
    14281 gcttttgata cagctttaac gaaatcccct ctgacctggg tcgcctccag catgccctta
    14341 atcgtacagt tctggttaat cgtgacattg ttgagcgttc ctgagttcgc attcacactg
    14401 ccactgatat ccgcattttt agcggtcagc tttccgtctg atgtcaggga aaataccgga
    14461 ggactgccac cgctggtaat ggtgggggcc gtcaggcgct tcaggaacac gtcgttcatg
    14521 aatatctggt tgccctgcgc cacaaacatc ggcgtttcat tcccgtttgc cgggtcaata
    14581 aacgcgatac ggttagcggc aaccagaaac tggctcagct tgccttcctc cgtgtcctcc
    14641 atgctgaggc caatacccgc gacataatgt ttgccgtctt tggtctgctc aattttgacg
    14701 ccccacatgg cattccattt atcgttggcg tctttccact ctttcgaaaa ctcctccagt
    14761 ttgctggcgt tatcctccgt cagctcgact ttttccagca gctccttgcc gagatgggat
    14821 tcggttatct ggcctttgaa aaaatccagg taaccttccg catcatcgct cgcccgaccg
    14881 acagcctcca caaatgccga tttgccaaca gtgttcacac tgcggatata aaagtaataa
    14941 tcatggcccg gttt
//
LOCUS       DAZFDP010000123        14923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFDP010000123 DAZFDP010000000
VERSION     DAZFDP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHJ1G_003734"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHJ1G_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4765511.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            46..315
                     /locus_tag="ACHJ1G_003735"
     CDS             46..315
                     /locus_tag="ACHJ1G_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765512.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            519..1637
                     /gene="ybdK"
                     /locus_tag="ACHJ1G_003736"
     CDS             519..1637
                     /gene="ybdK"
                     /locus_tag="ACHJ1G_003736"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4765513.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHJ1G_003737"
     CDS             1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHJ1G_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4765514.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHJ1G_003738"
     CDS             2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHJ1G_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4765515.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHJ1G_003739"
     CDS             2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHJ1G_003739"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4765516.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHJ1G_003740"
     CDS             3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHJ1G_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4765517.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHJ1G_003741"
     CDS             complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHJ1G_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4765518.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHJ1G_003742"
     CDS             complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHJ1G_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4765519.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHJ1G_003743"
     CDS             complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHJ1G_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4765520.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHJ1G_003744"
     CDS             complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHJ1G_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4765521.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHJ1G_003745"
     CDS             complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHJ1G_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4765522.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12446..13129
                     /gene="cusR"
                     /locus_tag="ACHJ1G_003746"
     CDS             12446..13129
                     /gene="cusR"
                     /locus_tag="ACHJ1G_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4765523.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13119..14567
                     /gene="cusS"
                     /locus_tag="ACHJ1G_003747"
     CDS             13119..14567
                     /gene="cusS"
                     /locus_tag="ACHJ1G_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4765524.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3619 a         3996 c         3672 g         3636 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gaactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg acttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagt
      421 caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
      481 tctatactga atagaaaacg ccaacttaag agaaacctat gccattaccc gattttcatg
      541 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
      601 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaatgag atcacggccg
      661 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
      721 atatcaacca ggctgccggg cagttttcag cgatgcagaa agtcgtattg caggcagccg
      781 cagaccatca tctggaaatt tgcggcggtg gcacgcaccc gtttcagaaa tggcagcgtc
      841 aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc tatctcatcc
      901 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
      961 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
     1021 cgccgtatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
     1081 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
     1141 ttcgctgcct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
     1201 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
     1261 gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta ctgacggaac
     1321 gcccgtttaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
     1381 gccgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
     1441 cggaagatac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa atgggtgcat
     1501 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
     1561 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
     1621 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
     1681 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
     1741 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
     1801 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
     1861 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
     1921 ctggcaacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
     1981 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
     2041 cggcgaaacg cctagccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
     2101 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
     2161 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
     2221 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
     2281 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
     2341 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
     2401 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
     2461 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
     2521 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
     2581 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
     2641 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
     2701 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
     2761 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
     2821 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
     2881 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
     2941 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
     3001 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
     3061 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
     3121 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
     3181 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
     3241 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
     3301 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
     3361 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
     3421 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
     3481 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
     3541 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
     3601 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
     3661 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
     3721 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
     3781 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
     3841 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
     3901 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
     3961 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
     4021 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
     4081 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
     4141 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
     4201 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
     4261 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
     4321 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
     4381 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
     4441 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
     4501 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
     4561 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
     4621 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
     4681 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
     4741 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
     4801 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
     4861 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
     4921 cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc aaacagcatg
     4981 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
     5041 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
     5101 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
     5161 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
     5221 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
     5281 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
     5341 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
     5401 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
     5461 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
     5521 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
     5581 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
     5641 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
     5701 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
     5761 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
     5821 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
     5881 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
     5941 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
     6001 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
     6061 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
     6121 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
     6181 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
     6241 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
     6301 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
     6361 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
     6421 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
     6481 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
     6541 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
     6601 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
     6661 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
     6721 gcgcattctc ggttttcagc attgacggtc cggtagagac tttaatgtcg gccacgtctg
     6781 ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt gcctgcggac
     6841 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
     6901 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
     6961 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
     7021 ccagacgctc ggctagagca gaagccacgc ctggcaccgt tcgcgctact tcttcaattt
     7081 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
     7141 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
     7201 ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc attgtcatgc
     7261 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
     7321 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
     7381 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
     7441 ccggcagcgt cgatggcata tacaacaggt cgccttcatt gatctgcggt aaaaattccc
     7501 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
     7561 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
     7621 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa cccatcagga
     7681 tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
     7741 ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
     7801 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
     7861 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
     7921 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
     7981 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
     8041 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
     8101 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
     8161 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
     8221 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
     8281 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
     8341 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
     8401 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
     8461 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
     8521 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
     8581 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
     8641 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgca
     8701 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
     8761 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
     8821 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
     8881 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
     8941 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
     9001 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
     9061 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
     9121 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
     9181 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
     9241 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
     9301 accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc gtttcggtac
     9361 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
     9421 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
     9481 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
     9541 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
     9601 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
     9661 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
     9721 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
     9781 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
     9841 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
     9901 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
     9961 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    10021 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    10081 gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca cccccagatt
    10141 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    10201 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    10261 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    10321 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    10381 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    10441 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    10501 agttttaatt tcactcattt tcgtctgcgg agtgatggta aagcgcatgg tcatctccgg
    10561 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    10621 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    10681 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    10741 ttgcagtgct tttttcattt attctctcct ggagttaaaa gtcatactct aaataattcg
    10801 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    10861 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat tgctgtcaac
    10921 cgccacctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    10981 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    11041 ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    11101 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    11161 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    11221 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    11281 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
    11341 cggttgatat tccgctggtc agccttatag atgggaaaaa tgccgcgcgt gcggccccaa
    11401 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    11461 agatttgcga cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    11521 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    11581 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag
    11641 cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat
    11701 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgctagct
    11761 gctgattgaa ataactttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg
    11821 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    11881 aaaaatcgag atcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    11941 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    12001 cggcatcggt cagacgatat tgcgcccgcg tttcctgcac tttcagcgtc gccatgcgca
    12061 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt tacctgatta tcaacaaaaa
    12121 aggtgcgcca gcccacgttc tggtagttat ctgctgcgtt aaccaagccg ttctggctga
    12181 gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac
    12241 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    12301 ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa
    12361 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    12421 tgcccgggca acatgcggag gaaaaatgaa actgttgatt gtcgaagatg aaaagaaaac
    12481 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    12541 cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat
    12601 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg
    12661 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    12721 gttgggggca gatgactacc tggtgaaacc attcgctttt gctgaactgc tggcgcgggt
    12781 gcgcactctc ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga
    12841 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    12901 cagtaaagag tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg
    12961 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga
    13021 tgtagcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agctaattca
    13081 gaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc
    13141 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcagcgt
    13201 ttttcgcctt tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata
    13261 ttaatgattt aaaagaaatt agcgccacgc ttgaacgggt acttaatcat cctgacgaga
    13321 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga
    13381 tctccctggc agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc
    13441 gcgagtttgc acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc
    13501 tttccggccc gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact
    13561 ggcggatgat taacttgtcg gttggcccgt tggtggacgg caaaccgatt tatacgctct
    13621 acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta
    13681 ttatgaccgc atcggtaatc agcatcctga tcgtctttat cgtactgttg gcggtacata
    13741 aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc
    13801 tcgacgttcg ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt
    13861 tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg
    13921 atatcgctca cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc
    13981 tcagccagtc tcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc
    14041 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc
    14101 agctaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    14161 ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg atttgttggc gacgaatgtc
    14221 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc
    14281 tgcgttatac gccgcccgga gaggcaattg tagtgcgctg ccagacggtc gatcaccagg
    14341 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt
    14401 ttgaccgttt ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt agcggcattg
    14461 ggctggcgat agtgaaatcg attgttgtcg cgcataaagg cacggttgca gtaacgtcag
    14521 atgcgcgggg gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttcaa
    14581 attcagggcg tggtgcatca cctcctggta ttgttgatca acaaattaga gaaaaactta
    14641 ttaaacatat gaaatatcga aggcttttat ttttcaaagt ttcacgagat gtttgtatat
    14701 gaaacttagt taaatctgtg tttcttccaa ttgtagatca caatgccctt tctaaatttt
    14761 catcgttata aacaagccat ctatcatata aagcaatgaa taatttaatc gcgattaatc
    14821 atcattaatt gttatattat tcactcaatc tatttatcaa gtgaatttga taaattccta
    14881 atttaaaata tccactattg agccagtatt taacaacgtt aag
//
LOCUS       DAZFDP010000124        14417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFDP010000124 DAZFDP010000000
VERSION     DAZFDP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..592
                     /locus_tag="ACHJ1G_003748"
     CDS             26..592
                     /locus_tag="ACHJ1G_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4765525.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHJ1G_003749"
     CDS             643..3102
                     /gene="ybgQ"
                     /locus_tag="ACHJ1G_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4765526.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHJ1G_003750"
     CDS             3117..3848
                     /gene="ybgP"
                     /locus_tag="ACHJ1G_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4765527.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHJ1G_003751"
     CDS             3845..4906
                     /gene="ybgO"
                     /locus_tag="ACHJ1G_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4765528.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5058..6104
                     /gene="abrB"
                     /locus_tag="ACHJ1G_003752"
     CDS             5058..6104
                     /gene="abrB"
                     /locus_tag="ACHJ1G_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4765529.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHJ1G_003753"
     CDS             complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHJ1G_003753"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO4765530.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHJ1G_003754"
     CDS             complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHJ1G_003754"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4765531.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHJ1G_003755"
     CDS             complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHJ1G_003755"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4765532.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHJ1G_003756"
     CDS             complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHJ1G_003756"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4765533.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHJ1G_003757"
     CDS             complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHJ1G_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4765534.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHJ1G_003758"
     CDS             10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHJ1G_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4765535.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHJ1G_003759"
     CDS             complement(11794..13212)
                     /gene="phrB"
                     /locus_tag="ACHJ1G_003759"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4765536.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHJ1G_003760"
     CDS             complement(13209..13718)
                     /gene="ybgA"
                     /locus_tag="ACHJ1G_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4765537.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13869..14417)
                     /locus_tag="ACHJ1G_003761"
                     /pseudo
     CDS             complement(13869..14417)
                     /locus_tag="ACHJ1G_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3466 a         3854 c         3506 g         3591 t
ORIGIN      
        1 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac agtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgatgtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     1861 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga
     2461 gcccaggtga gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacatcgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactacagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac
     4441 tgcccaaaac ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4741 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4801 ggtctacgaa aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4861 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4921 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4981 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5041 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5101 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5161 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5221 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5281 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5341 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5401 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5461 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     5521 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5581 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5641 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     5701 cagtgctcca gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg
     5761 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5821 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg
     5881 gtatggcgtg ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca
     5941 ccagccccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6001 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca
     6061 ttgcacggtt tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc
     6121 accagtaaaa cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc
     6181 acggttcgcc atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6241 catccacctg cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga
     6301 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     6361 gccagaggat ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca
     6421 gtaatcctgc aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct
     6481 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6541 tcagttgttc cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6601 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6661 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat
     6721 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6781 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6841 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6901 ccttttttca ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa
     6961 tgtagctcgt aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac
     7021 cgtttgcgcc gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7081 catctccagc gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact
     7141 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7201 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat
     7261 caatgccgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7321 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7381 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7441 cggcaactgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     7501 aattgcaaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc
     7561 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     7621 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta
     7681 attgttcaaa ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7741 ggacaaaatg aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa
     7801 tgatgcaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     7861 gctgcccgtt atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc
     7921 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     7981 agctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     8041 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     8101 cttccataaa atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     8161 ggccttccag cccgccactc cccactttga gatcggtgct gcatgacccc attaccggcg
     8221 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     8281 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     8341 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     8401 cagttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta
     8461 ggttagcaat gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc
     8521 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     8581 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     8641 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     8701 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8761 aacgagtaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8821 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8881 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     8941 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     9001 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     9061 acgcaaaatc accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     9121 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     9181 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     9241 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     9301 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     9361 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     9421 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     9481 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     9541 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     9601 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9661 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     9721 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9781 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9841 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9901 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9961 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
    10021 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
    10081 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
    10141 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
    10201 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
    10261 tggaattatt atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct
    10321 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
    10381 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
    10441 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
    10501 ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga
    10561 gatccatccg tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt
    10621 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10681 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc
    10741 ctgtggttac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10801 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10861 cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct
    10921 ggttctgctg gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt
    10981 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    11041 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    11101 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
    11161 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    11221 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    11281 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc
    11341 cggattctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
    11401 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
    11461 tgccatgtcg caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat
    11521 ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac
    11581 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    11641 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11701 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag
    11761 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
    11821 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
    11881 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    11941 gcgcagctcc ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg
    12001 ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca
    12061 ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    12121 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg
    12181 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg
    12241 gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac
    12301 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
    12361 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    12421 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    12481 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
    12541 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    12601 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    12661 cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc
    12721 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    12781 attaccggtc ttcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
    12841 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
    12901 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    12961 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
    13021 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    13081 cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg
    13141 gcaggcggca gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag
    13201 atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
    13261 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
    13321 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc
    13381 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg
    13441 gtgtgatcgc gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg
    13501 ttgtaaaaat catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
    13561 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
    13621 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata
    13681 cggattaggg tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca
    13741 ggagtaagtg tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca
    13801 aatttacgaa agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc
    13861 cctttctatt caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga
    13921 cgggtttctt ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta
    13981 tttaattctt tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct
    14041 tttacagcga ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg
    14101 caacccatcg catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct
    14161 gggatagctg tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg
    14221 tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa
    14281 gagtgccgga gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa
    14341 tcacgcatcc agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact
    14401 ctggcaatag tatcgtg
//
LOCUS       DAZFDP010000125        14283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFDP010000125 DAZFDP010000000
VERSION     DAZFDP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..278
                     /locus_tag="ACHJ1G_003762"
     CDS             <1..278
                     /locus_tag="ACHJ1G_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO4765538.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHJ1G_003763"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHJ1G_003763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO4765539.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLREFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHJ1G_003764"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHJ1G_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO4765540.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHANIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6369..6929
                     /locus_tag="ACHJ1G_003765"
     CDS             6369..6929
                     /locus_tag="ACHJ1G_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO4765541.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPQLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7048..7260
                     /locus_tag="ACHJ1G_003766"
     CDS             7048..7260
                     /locus_tag="ACHJ1G_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4765542.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7547..8224
                     /locus_tag="ACHJ1G_003767"
     CDS             7547..8224
                     /locus_tag="ACHJ1G_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFO4765543.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQ
                     NVVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDK
                     GYDGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQ
                     QIAGGGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKK
                     AKKENKA"
     gene            8890..9081
                     /locus_tag="ACHJ1G_003768"
     CDS             8890..9081
                     /locus_tag="ACHJ1G_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765544.1"
                     /translation="MNGFRNSSRNGQVWRYQSAGSRAVILEAAEAWRRAAGVAPRTDW
                     QQFARKRAEQCHRRCRGRV"
     gene            9325..9786
                     /locus_tag="ACHJ1G_003769"
     CDS             9325..9786
                     /locus_tag="ACHJ1G_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO4765545.1"
                     /translation="MMKYIPLVLFIFSWPVLSEDIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVFQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            9832..10041
                     /gene="hha"
                     /locus_tag="ACHJ1G_003770"
     CDS             9832..10041
                     /gene="hha"
                     /locus_tag="ACHJ1G_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4765546.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            10079..10669
                     /locus_tag="ACHJ1G_003771"
     CDS             10079..10669
                     /locus_tag="ACHJ1G_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO4765547.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10909..11166
                     /locus_tag="ACHJ1G_003772"
     CDS             10909..11166
                     /locus_tag="ACHJ1G_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4765548.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            complement(11202..11354)
                     /locus_tag="ACHJ1G_003773"
     CDS             complement(11202..11354)
                     /locus_tag="ACHJ1G_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO4765549.1"
                     /translation="MAPVIFSQSPNLTKIIGVFTFLAPVNPHQNQFPATLRRGQPQNS
                     LKRSAI"
     gene            11403..11477
                     /gene="tap"
                     /locus_tag="ACHJ1G_003774"
     CDS             11403..11477
                     /gene="tap"
                     /locus_tag="ACHJ1G_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4765550.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            11470..12327
                     /gene="repA"
                     /locus_tag="ACHJ1G_003775"
     CDS             11470..12327
                     /gene="repA"
                     /locus_tag="ACHJ1G_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4765551.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            13243..13512
                     /gene="yacA"
                     /locus_tag="ACHJ1G_003776"
     CDS             13243..13512
                     /gene="yacA"
                     /locus_tag="ACHJ1G_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO4765552.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            13512..13622
                     /gene="yacB"
                     /locus_tag="ACHJ1G_003777"
                     /pseudo
     CDS             13512..13622
                     /gene="yacB"
                     /locus_tag="ACHJ1G_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            13721..>14283
                     /locus_tag="ACHJ1G_003778"
     CDS             13721..>14283
                     /locus_tag="ACHJ1G_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765553.1"
                     /translation="MTIESFFIGTRRSDKRYGPQSKDMQVSEFISLISPKNAPHKVVL
                     PDFTGLAIRLDAQIRNQFHQLKEDEHFLRYRQLSERWYQAGSISDRNNRSKRFEKIMD
                     DSLDFLLYSQDVMPNINPDDLQWHDYEKASSKGKMYCVALLFHVIARAAYEPESVGKD
                     PTLPEYCRWMKNWIEKTLGHDFLDRMM"
BASE COUNT         3625 a         3345 c         4234 g         3079 t
ORIGIN      
        1 tgtcttctgt catcaatgat aagaagtcag atgcaggtgt gagtgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc
      181 atccggacat ccagcagcat atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
      301 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggcag
      361 catgggagaa cgctgggctg gtcagggggc tgaacagctt gggctgcagg gcagcgtcga
      421 taaggatgtt tttacccgtc ttctggaggg caggctgccg gacggggcgg atttaagccg
      481 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgctcggggg ggataaacgt cttattgatg cacataacca
      601 ggccgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
      661 cggacagtca gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accatgacac
      721 cagtcgcgat caggacccac agttacacac gcatgtagtg gtggctaatg tcacgcagca
      781 taacggcgag tggaagacac tgagcagtga caaagtgggg aaaaccggat tttccgagaa
      841 cgtgctagcg aaccggattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
      901 tgaggcgctg ggttatgaaa ccgaagtggt cggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccagtcgttc acaggctatc agggaggccg tcggtgaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcatat cgtgacgcgg cggatcagcg tgcggagatc cgcacgcagg cgcccgggcc
     1201 tgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtatacgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaaaat ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgacc gtgagaaggg gctgttcaca tcaggaattc atgtgctcga
     1441 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca
     1501 tccggagaaa agtgtccccc ggacggctgg ttacagcgat gcggtcagtg ttctggcaca
     1561 ggaccgtccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc
     1681 tgaccgtcgc tcgcagatga acctgaagca ggatgaacgg ctgtccggtg agctgataac
     1741 cggacgtcgt cagctgcagg agggcatggt cttcacgccg ggcagtactg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     1921 ggccatgaag gatgccgggg tgaacacgta tcgctggcag ggcggagaac agcgaccggc
     1981 aaccatcatc agtgaaccgg accggaatgt ccgctatgac cggctggccg gagattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     2101 catactgaca caggccattc gcagtgagct gaaaacacag ggcgtgctcg gacacccgga
     2161 ggtgaccatg accgccctgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccga ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cggcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 cgaaacgcag gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggccaccct gcctgtggct gattcaccgt tcacggcact
     2581 gaagctggag aacggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgacggt
     2641 gtttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     2761 ccgccatccc tcctttacgg tggtttctga gcagataaag gcgcgggccg gtgagacatc
     2821 gctggaaacc gctatcagtc tgcagaaaac cgggctgcac acgccggcac agcaggccat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccggtttt actgaactgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgacttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaaa agtattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagagta cccggcgaac tcatggagac
     3181 gttaacgtcg ggacagcgtg ccgccacccg tatgatactg gaaacgtccg accgtttcac
     3241 ggtggtacag ggttatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaacatg ctgccggcga gtgagcgtcc ccgagttgtg gggctggggc ccacgcaccg
     3361 tgcggtcggt gagatgcgca gcgccggcgt ggatgcacag acgctggcgt cctttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggc ggtcgtgccg tggccagtgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca tcatgaagga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggttcccc gtcaggaggg
     3781 cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg tgagactttt ccggatgtcc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct
     3961 gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga acacagcgaa
     4081 tatacgtgac ggggagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 tgtcacactg tacacaccag acaaaatccg ggtggggacc ggcgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggccagga
     4441 gcgggcagag caacatattg acctggccta tgccatcacc gcccacggtg cgcagggtgc
     4501 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg
     4561 ctttgagtca gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccatcaa caatgccgta cagaaaggaa cggcccacga
     4681 tgtgtttgaa ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 acgggaactg cgggacgtgg cggcaggccg tgccgttctc cgtcaggcag ggctggccgg
     4801 gggagacagt cctgcacggt ttattgctcc gggacgtaaa tatccgcagc cgtatgtggc
     4861 actgccggcg tttgaccgta acggcaggtc cgccggtatc tggctgaacc cactgaccac
     4921 ggatgacgga aacgggctgc gggaattcag cggtgaagga cgggtgaaag gcagcgggga
     4981 tgcgcagttc gtggccctgc agggcagccg taacggagag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa tcctgacagc ggtgtggtgg taagaattgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggtcgcgtgt ggggggatat
     5161 cccggacagc agcgtccagc cgggagccgg aaatggcgaa ccggtcacgg cagaggtgct
     5221 ggcacagcgg caggctgaag aggccatccg ccgtgaaacg gaacgccgcg cagatgaaat
     5281 tgtcagaaaa atggcagaga acaaacctga cctgccggat ggtaaaacag agcaggctgt
     5341 cagggaaatt gccgggcagg agcgtgaccg ggctgacata actgaacggg aagccgcgct
     5401 gccggagagt gtgctgcgtg aatcacaacg ggagcaggaa gcggtcaggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gtctgcagca gatggagcgg gatatggttc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagaca aacactgatg acaacggata
     5581 acacgaacac gacacgtaac gattcactgg ctgcacggac cgatacctgg ttgcagtcat
     5641 ttctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg
     5701 tactggacca catcaatctg atattccagc tgaagcagga atggatgttt ctggccgggc
     5761 gaggagcctt tccgctgttt gccctggtgt gggggctgaa tctgtcccgt catgcgaata
     5821 tccggcaacc ggccattaac cggctgtggg gatgggggat tattgcccag ttcgcatatt
     5881 atctggccgg ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggcggcac
     5941 aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc attcttctga
     6001 tggccctgtg ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg
     6061 cagtcagcca ccggctgtac cgggcagaag acagagcgga acgtctggcg ctggtcgcct
     6121 gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggctg
     6181 gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc
     6241 gtttctggcc cggggatttt ttcccggtat tctacgcctg tcatctggct gtgctgggcg
     6301 ttctggcgct gtgacgggtg tggtatcttt ggccgcaaga ggatgattcg tcagaggcag
     6361 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa acggaagggg
     6421 agacgcctgt ccgcagccgg aaaaccatca tcaatgtcac cacgccacca aagtggaagg
     6481 tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg gcaaaaaaag
     6541 cgcaggccag acaggcactg tccatttatc tgaacctgcc cacgctggat gaggccgtga
     6601 ataccctgaa gccctggtgg ccgggattgt ttgacggtga cacaccccag cttctggcct
     6661 gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata
     6721 aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga
     6781 aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg
     6841 aggaagtgta tgcggcagcg cgtctggata aaatccgccg ccagaacagg ataaaggcag
     6901 agcttcaggc cgtgcttgat gagaaataaa aaaaagcctc catcagagag gaggcaggga
     6961 aataaggctt aaaaggaaac caaaggaagc caattcagta gattatttaa caccataata
     7021 ctgacgggtt aaagaggagt gataaagatg aatggattca gaaacagttc acggaacggt
     7081 caggtctggc gctggcagcg tgccggaagc cgggccgtta ttctggaggt cagtggacgc
     7141 tggatggaag cggcagaagc atggcgacgg gctgccggtg ttgcgccccg gacagactgg
     7201 cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gcagccgggg aagggtgtaa
     7261 cgaaaaaagc agccttcatt actccaggga ttcagaaggc tgaagtatgc atgagatttt
     7321 tcttttttat atctggcctg gcagtatgac cagtcagcat tcattatcac aggattattg
     7381 ctgtattaag taataacgtg ctgttttgtg attgatttca taaaacaaga cgtcgtttaa
     7441 tatgcgcctg aatattcacg agggaagtct ttctttttat atggtgttcc cggaaataaa
     7501 atattttgtg ttattgctca cccggttatg aataaaaggt aaaaacatgg aagaaaataa
     7561 tacgagtaca gataaaaaca ccctgacagc ccttattgat tatgaaaact gtggcagtct
     7621 gaaaaatatt tcccttgagc agtatgggga actgattatt tttgtcgggc cacagcagaa
     7681 cgtcgttgtt cttcctgcag actcttttcc tgaaggtgcg agaatcacta tcaggcaggt
     7741 gagtggtgtg tccaggaata atgtcgattt tcatcttgtt ctggagctgg ggcggataag
     7801 ctgttgtgca gcagggaaag ataaaacata ccacatcatt tcatcggata agggatatga
     7861 cggtgttatc aggacgctga aagaagccgg tattacctgc atccgtcgtg atgtaccgga
     7921 aagtattgct gctgccggtg agtctggtgc agacaaaagt aactgcctga aacaaccttc
     7981 tgttcctgaa agtgatatcg tcagccgttg ggcaggaaag ctgcagcaga tagccggcgg
     8041 cggagccggt aatatgcctg tgtccgtcga aacatttaat aattatttaa agtcacacat
     8101 gcgtgacgaa tggaacaaaa cccgggcagt ggcgataagg aatgaactgg ttcgtcgggg
     8161 ggtcacagaa gtcagggaga aaaagataat ctggaaaaag gcgaaaaagg agaataaagc
     8221 atgaggtatc gcagctgtac tcagataaaa acagcccccg ttgcggttgc ggagcgaagg
     8281 gggctgaaaa ggggaattaa ggatgattgt tattatggtg aaccagaagg ttatccctgt
     8341 ctgtaagctg aatcggacta aacgacataa tacctgtaac aatacctgta acatattttc
     8401 ctggcaggac aagcaggaac aataaaatgt gtgatttaat tcaaaaaatg tgcgcggcaa
     8461 agaaaaaccg gggaattgct tccccggtcc ggtccattta gccagttccc tgagcaggat
     8521 ctgatatgag cattattcac tttgccagac atggtgaaaa aagtgaatat atggaggaga
     8581 aaaacatcgc ctgaatcaca gattcgggtg ctcagcgcgt aagaagacgg gaaagaaaag
     8641 cgcctttccg tttcagcatc gggaataatc actgaaacat cagcagtata tgtggtgtgt
     8701 atgaatatcc cggcagcatt aattcttctg tgaggaaatg cattgataag aaacagaata
     8761 agtcggtggc aaggaaaatg aatttattaa ttcagcatcc gtttatcaga taaaaaaggg
     8821 aaagagtatg gtgaaactga ttatttttat tatcacaata atactgacaa ctaaaggggg
     8881 gacaggaaaa tgaatggatt cagaaacagt tctcggaacg gtcaggtctg gcgttaccag
     8941 agtgccggaa gccgggccgt tattctggaa gcggcagaag catggcgacg ggctgccggt
     9001 gttgcacccc ggacagactg gcagcagttt gcccgaaaaa gggctgaaca atgccaccgg
     9061 cgctgccggg gaagggtgta acgaaaaagc agccttcatt actccaggga ttcagaaggc
     9121 tgaataatgc atgagatttg ctttttttaa tatccggtcg ggcagtatga ccggacagtt
     9181 ctgagtagca cagagttttt tccgtattaa gtcgtgattt tatattttgt gatttatttc
     9241 acaaaataag gtgttattca ttgtgtgctg caatgttcag gatgcctgaa atacaggtgc
     9301 catttatctg atatggagaa taacataatg aaatatattc cactggtatt atttatcttt
     9361 tcatggccgg tattgtctga agatattcat ggacgggttg ttcgtgtact ggacggtgat
     9421 accatcgagg tcatggactc acggaaggcg gtgcggatca ggctggtaaa tattgatgca
     9481 ccggaaaaga aacaggatta tggccggtgg tcaacggaca tgatgaaatc actggttgca
     9541 ggaaaaacgg ttaccgtcac atattttcag cgagatcgtt atggtcgtat tctcgggcag
     9601 gtttatgcac cagatggaat gaatgttaat cagtttatgg tccgggccgg ggctgcctgg
     9661 gtatatgagc agtacaacac agatcccgtg ctacctgtct ttcagaacga agccagacaa
     9721 caaaaacgtg ggctctggtc agatgctgat ccggttcccc cctggatatg gagacaccgt
     9781 aaataaagcg ctggcaatct tatcattcat ctgttctcag cgggaataaa aatggcaaaa
     9841 acaaaacagg aatggctgta tcagttacgg cgatgttcat cagtcaatac gctggaaaaa
     9901 attattcata aaaacagaga cagtttatta aacagtgaac gtgaatcatt taattcagca
     9961 gcagaccacc ggttagcgga attaatcacc ggaaagttat atgacaggat accaaaagag
    10021 atatggaaat atgtaaggta ataattcaga ataattaatt accaggagca gataaacaat
    10081 gaagctgatt atttttattc tcattgttct gattattgca gcgttactta tccggattat
    10141 cctcagaagt gtaaatcagc attctccttt gctgatgcag ttgcatgcgg caggtatacg
    10201 gaccggtgat gcagagcgaa tactgtccgg cggtgaatac tggcaacgtc agaagacgct
    10261 gctgacagaa agggaagtca gttttatgaa aggactgttc agaattgtgg atatgaagcg
    10321 gtggtatctg tgtccgcagg tacgggtcgc ggatatcgtc cagctgaacg ggaatatccg
    10381 gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc atgttgatgt
    10441 ggttatcgtt gagcggcgtt cgttcagtat tgttgcagca gtagagctgg atgatgccag
    10501 ccatttacga ccggaacgca gacgccggga tattcttctg gaagaggttc tgaggcaggc
    10561 tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga ccggagaatg
    10621 gctgaataca acaggggctg atcagcagtc cccggaacat cgtagctgac gccttcgcgt
    10681 tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc
    10741 cataactgaa aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt
    10801 tgatttgtgc gccaacacag atcttcgtca caattctcaa gtcgctgatt tcaaaaaact
    10861 gtagtatcct ctgcgaaacg atccctgtct gagaagagag gaggcgagat gtcgcagaca
    10921 gtaaatgcag agacttcctc atcgggaaaa aaacgacctt acagaagggg taatcctgtt
    10981 cctgcgagag aacgacaaaa agcgtctctt gcaagaagga gtgccactca taaagcgttt
    11041 catgccgtca ttcagcttcg actgaaagaa aagttaagtg agcttgctga tgaagacggg
    11101 atcactcagg cacagatgct tgagtggctg atagagtcag aggttaagcg cagaaaatct
    11161 ttgtgaatat ttgcgtttct tgctgtttca gtgatgagat gttagattgc tgatcgtttt
    11221 aaggaatttt gtggctggcc acgccgtaag gtggcaggga actggttctg atgtggattt
    11281 acaggagcca gaaaagtgaa aaccccgata atcttcgtca agtttggcga ctgagagaag
    11341 attaccgggg ccatctaaaa accgtatagc caacaattca gctatgcggg gagtatagtt
    11401 atatgcccgg aaaagttcaa gacttctttc tgtgctcact ccttctgcgc attgtaagtg
    11461 caggatggtg tgactgatct tcaacaaacg tattaccgcc aggtaaagaa cccgaatccg
    11521 gtgtttacac cccgtaaagg tgccggaacg ctgaagttct gcgaaaaact gatggaaaag
    11581 gcggtgggct tcacttcccg ttttgatttc gccattcatg tggcgcatgc ccgttcgcgt
    11641 ggtctgcgtc gacgcatgcc accggtgctg cgtcgacggg ctattgatgc gctcctgcag
    11701 gggctgtgtt tccactatga cccgctggcc aaccgcgtcc agtgctccat caccacgctg
    11761 gccattgagt gcggactggc gacggagtct gctgccggaa aactctccat cacccgtgcc
    11821 acccgtgccc tgacgttcct gtcagagctg gggttgatta cctaccagac ggaatatgac
    11881 ccgcttatcg ggtgctacat tccgaccgac atcacgttca cactggctct gtttgctgcc
    11941 cttgatgtgt ctgaggatgc agtggcagct gcgcgccgca gtcgtgttga atgggaaaac
    12001 agacagcgca aaaagcaggg gctggatacc ctgggtatgg atgagctgat agcgaaagcc
    12061 tggcgttttg tgcgtgagcg tttccgcagt taccagacag agcttaagtc ccgtggaata
    12121 aaacgtgccc gtgcgcgtcg tgatgcgaac agggaacgtc aggatatcgt caccctggtg
    12181 aaacggcagc tgacgcgtga aatctcggaa gggcgcttca ctgctaatgg tgaggcggta
    12241 aaacgcgaag tggagcgtcg tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac
    12301 agccggctgg ccacagcttc tccctgaaag tgatctcctc agaataatcc ggcccacgcc
    12361 ggaggcattc gcacgcctga agcccgtcag tgcacaaaaa atcagcacca catacaaaaa
    12421 acaacctcac catccacctt ctggtgcatc cggttccccc tgtttttaat acaaaacacg
    12481 cctcacagac gggtaattgt gcttatccac atttaactgc aagggacttc cccataaggt
    12541 tacaaccgtt catgtcataa agcgccagcc gccagtctta cagggtgcaa tgtatctttt
    12601 aaacacctgt ttatatctcc tttaaactac ttaattacat tcatttaaaa agaaaaccta
    12661 ttcactgcct gtcctgtgga cagacagata tgcacctccc accgcaagcg gcgggccccg
    12721 accggagcca ctttagttac aacactcaga cacaaccacc agaaaaaccc ctgtcctgcg
    12781 cagaaccgaa acaacaaagc ccctccctca taactgaaaa gcggccccgc cccggcccaa
    12841 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctatactccg tcaccgctgt
    12901 cagtcttctc gctggaagta ttgagtacac gctcgtaagc ggccctgacg gcccgctaac
    12961 gcggagatac gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacaga
    13021 agctctatca tggctgaaaa cgggtatggc ttagcaggac tggggatggg taaggtgaaa
    13081 tctatcaatc agtaccggct tacgccgggc ttcggcggtt ttactccggt atcacatgta
    13141 accacagcgt gccgccttcc atgccgctga cgcggcatat tctggtgacg atatctgagt
    13201 cgttatatac tgtgtatata cgtagtaatg acgaggtgat aaatggcaca ggttaatatg
    13261 agtttaagaa tcgacgctga actgaaggat gcttttatgg ctgctgcaaa aagcatggac
    13321 cgtaatggct ctcagttaat ccgggatttt atgcgtcaga ccgttgaacg gcagcataat
    13381 acctggttcc gtgaccaggt tgaggcagga cgtcagcaac tcgagcacgg cgatgtactt
    13441 ccccatgaca tggtcgaatc ctctgctgca gcatggcgag atgaaatgag caggaaggtt
    13501 gccggtaaat gatggagata ttctggacca tgctggccag ccaggacagg aagcgcattc
    13561 gtgaatacat tgcggagcag aacctgatag cagccattga actggacgaa cggattggtt
    13621 atttcggacg aacgcaccgg cttacgccgg ttttacatct gtactacatg taacctcata
    13681 cacacataaa tgagtaacat actgattaat atggtaaatc atgacgattg aaagcttttt
    13741 tattggcacc cgccgctctg ataaacgtta cgggcctcag agcaaggata tgcaggtttc
    13801 tgaattcatt tctctgattt ctcccaaaaa tgcccctcat aaggttgtgt tacctgattt
    13861 tactggtctg gcaattcggc ttgatgcaca aatcaggaac cagtttcatc aactgaaaga
    13921 ggatgagcac ttccttcgat atcggcagtt gtcggaaagg tggtatcagg cgggctctat
    13981 tagtgacaga aacaaccgca gtaagcgttt tgagaagatc atggatgact cacttgattt
    14041 tttgctttac agccaagacg tcatgcctaa catcaatcca gatgatctcc aatggcatga
    14101 ttacgaaaag gctagcagta agggtaaaat gtactgtgta gcgcttctgt tccatgtcat
    14161 tgcacgggct gcatatgaac ccgaatctgt gggtaaagat ccgacacttc cagaatattg
    14221 ccggtggatg aaaaattgga tagagaaaac attaggtcat gattttttag atagaatgat
    14281 gat
//
LOCUS       DAZFDP010000126        14177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFDP010000126 DAZFDP010000000
VERSION     DAZFDP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..98
                     /locus_tag="ACHJ1G_003779"
     CDS             <1..98
                     /locus_tag="ACHJ1G_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO4765554.1"
                     /translation="TLLLTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            122..4636
                     /locus_tag="ACHJ1G_003780"
     CDS             122..4636
                     /locus_tag="ACHJ1G_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO4765555.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4637..6286
                     /gene="yfaQ"
                     /locus_tag="ACHJ1G_003781"
     CDS             4637..6286
                     /gene="yfaQ"
                     /locus_tag="ACHJ1G_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO4765556.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6291..7067
                     /gene="yfaP"
                     /locus_tag="ACHJ1G_003782"
     CDS             6291..7067
                     /gene="yfaP"
                     /locus_tag="ACHJ1G_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4765557.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7342..10191
                     /gene="rcsC"
                     /locus_tag="ACHJ1G_003783"
     CDS             7342..10191
                     /gene="rcsC"
                     /locus_tag="ACHJ1G_003783"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4765558.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10277..10927)
                     /gene="rcsB"
                     /locus_tag="ACHJ1G_003784"
     CDS             complement(10277..10927)
                     /gene="rcsB"
                     /locus_tag="ACHJ1G_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4765559.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(10944..13616)
                     /gene="rcsD"
                     /locus_tag="ACHJ1G_003785"
     CDS             complement(10944..13616)
                     /gene="rcsD"
                     /locus_tag="ACHJ1G_003785"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4765560.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3431 a         3461 c         3801 g         3484 t
ORIGIN      
        1 ttactctcct tctgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
       61 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
      121 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgcgccgcc
      181 cgccggggga acattctttt tgcttgctga cagcagtttt agcagcagtg aagaggcgaa
      241 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
      301 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
      361 cctgcatcgc attgtggtgc aaccgcaata tctgggcgac gggctgaaca atacgctgac
      421 ctggctgtgg gataactggt acggcaaatc tcgccgcgta atgcagcgta ctttctcttc
      481 tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca atgccattat
      541 taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat atcccttggt
      601 ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc aaggggtaaa
      661 actggaaggc gcatccagca atttcatctc gccgcagccg ggtaacattt atattcctct
      721 cggccaacaa gagccgggac tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac
      781 gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggca acgagcttct
      841 ggtgtggacc gcgggtaaaa aacagggtga agcgaagccc ggctcagaga tcttgtggac
      901 tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac agcggtacct tgcagttaca
      961 acatatatcg ccagaacgtt catacattct gggtaaggat gctgaaggcg gtgtttttgt
     1021 ctccgagaac ttcttctacg aaagcgaaat ctacaacacc cgcttgtata tttttaccga
     1081 tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
     1141 cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg tgctggacgc
     1201 taacggcagt ctgttgcaaa ccgtcaatgt cacgctggat gcgcgcaatg gcgggcaggg
     1261 aagtttccgc ctgccagaaa atgccgtagc cggaggttat gagttacgtc ttgcttaccg
     1321 caatcaggtc tatagcagca gttttcgcgt ggcaaactac atcaagccac atttcgagat
     1381 tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
     1441 actgctttac ccggatggtg agccggtaaa agatgcccgc gtgcagttaa gtttgcgcgc
     1501 tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgttttc ccgtgtcgct
     1561 ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc
     1621 cgataaaccg agccgctatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt
     1681 caccaccacc aaagagatcc tcattgaacg cggcctggcg cattactctt taagtaccgc
     1741 cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc
     1801 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg
     1861 agagctaccg tcaggcggca aatcctttac cgtcaatttc gctaaacctg gcaactacaa
     1921 tctgacatta cgcgataaag acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg
     1981 taagggcagc acggcgcata ctggtacggt agatatcgtg gcggataaaa cgctgtacca
     2041 gccaggcgaa actgcgaaga tgctgattac ctttccggag ccaattgatg aagcattatt
     2101 gacgctggaa cgcgatcgcg tggaacagca gtcgctgctt tcgcatccgg caaactggct
     2161 gacgttacaa cgtttaaacg atacccagta tgaagcccgg gttccagtga gcaattcctt
     2221 tgcgcctaac atcacttttt cggtgctgta tacccgtaat ggtcagtaca gttttcagaa
     2281 cgccgggatc aaagttgccg ttcctcagct tgatatccgg gtgaaaacgg acaaaaccca
     2341 ttaccagcct ggtgaactgg tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt
     2401 ttctgcgcag ctaacggtag gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat
     2461 cgcgccgaat atcggcaaat ttttctatcc gctggggcgt aacaatgtgc gtaccagctc
     2521 cagtctgtcg tttatcagct acgaccaggc gctctccagc gagccggttg cgcctggcgc
     2581 aactaaccgc agcgagcggc gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga
     2641 taccgcggca tggatgccgt cactcacaac cgataaacaa ggcaaagcgt atttcacgtt
     2701 cctgatgcct gattcgttaa cccgctggcg tatcaccgcg cgtgggatga acggcgacgg
     2761 gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa aatctctaca tgaagtggag
     2821 tatgccaacg gtgtatcgcg tgggcgacaa accggcggca ggactgttta tcttcagtca
     2881 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggcgctgaga tgcgccagac
     2941 gctgacgctg cacaaagggg cgaattatat ttcgctgacg cagaacattc agcaatctgg
     3001 cttgttaagt gcagaactgc aacaaaatgg gcaagtgcag gacagcatta gcacaaaact
     3061 gtcttttgtg gacaacagct ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg
     3121 cgagaacgcg ctgatgttgc ccgagcaggc gagtaatatc cggctacaaa gtagtgaaac
     3181 gccgcaggag attttccgca acaatcttga tgcgttagtc gatggaccat ggggtggcgt
     3241 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
     3301 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
     3361 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggtgacgc
     3421 cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
     3481 gcaacaaccg gaatactggc agcatatgct cgacagttat gccgagcagg cggataacat
     3541 gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa
     3601 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaag atgaagattt
     3661 ctctgaggaa gacatccgcg atatcaatga cagcctgatc ctcgatacac cggaatctcc
     3721 actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa
     3781 gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagcaatca
     3841 gccgctggcg cacacggttg tgctgctcaa tagcggtggt gacgctaccc agacggccgc
     3901 tattttaagt ggtttgaccg ctgagcaatc cactattgag cgcgcgctgg ccatgaactg
     3961 gctggcgaaa tatatggcga caatgcctcc agttgttttg cctgcgcctg cgggcgcatg
     4021 ggctaaacat aagttaactg gagggggcga agactggcgt tgggttggtc agggcgtgcc
     4081 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
     4141 gccggcaaaa atggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
     4201 gcttatccct ggtgaagaag agatgagctt tattctgcaa ccggtgacca gcaatgagat
     4261 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
     4321 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
     4381 gggcatttca gtcaataaac ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc
     4441 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga cgggaacggt
     4501 cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg
     4561 ttatgtgcgt tcctatgcac ctgcgcagca aagtgttgcg gcagggagtg aatggaccgg
     4621 gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactct
     4681 gccaacgctt gcggaggaga cgccgttaca actggtgctg cgcggtgcgc agcacgatca
     4741 actgtatcag ctgtcgtcat caggcgtgac caaagtctca gcattacctg actcgctcac
     4801 cacgccgtta gggagtttgt ggaaactgta tgtttacgcc tggctggagg atacccatca
     4861 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
     4921 tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct attttgcgcc
     4981 gcagcggttg catattggcg cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc
     5041 tgcgtggtta gcgtctctta caacgctgaa gccagaaacc tcggtaacgg ttaaatcgct
     5101 gctcgattca ttagcgacgt tacctgcgca aaacaaagct caggaagtct tactggatgt
     5161 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
     5221 aacctggagc tggtttgccg atgataaaca ggaaatccgc cagggtggtt ttgctggctg
     5281 gttaaccgat ggtacaccgc tgtgggttac tggcagtggc accagcaaaa cggtactaac
     5341 ccgttatgcg acggtgctta atcgggtttt gccagtgccg acgcaggttg ccagtgggca
     5401 atgtgtagag gtggagctgt ttgcccgcta tccgctgaag aaaatcacag cggaaaagag
     5461 tacgacggca gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacgt ttactaatgg
     5521 taaccacatt acgtttgtca gccacggtga gacgacctta ctgagcgaaa agggcaaact
     5581 gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
     5641 gaaaagtacg ccccctgagg cagcgaaagc catgaccgtg gcgattcgca cttttttaca
     5701 gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg ccacgcagcg
     5761 tgtttctgct tcgccagcga ccacgggggc gcgaacgatg accgcctgga cgcaggatct
     5821 catctatgca ggcgatccgg ttcattatca tggcagtcga gctaccgaag ggacactttc
     5881 ctggcggcaa gccacggccc aggcggggca gggagagcgt tacgaccaga tactcgcgtt
     5941 tgcttatccc gacaacagcc ttagccgctg gggagcgcca cgctcaacct gccagttatt
     6001 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
     6061 cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
     6121 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
     6181 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
     6241 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
     6301 tttttcttcc gttgttactg gtggcacttt cgcctgtcgc tcacagtgaa ggtgtgcagg
     6361 aagtcgagat tgacgcaccg ctctctggct ggcatccggc agagggcgaa gatgccagtt
     6421 tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
     6481 cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggtc
     6541 ggctggtggt caacggtgcc agtatgccgc agagaattga atccgatggt tcatttgcac
     6601 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa
     6661 gccggcaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt gcacgtttac
     6721 ggctggttct ctcgtgggat acggacaata ccgatctcga tctccatgtt gtcacgcccg
     6781 atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg
     6841 atgtcacgac ggggtacgga cccgagattt tcgccatgcc agcaccagtt cacgggcgct
     6901 atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattgacg accgcccaac
     6961 tgacgctgat caccgatgaa ggatcggtca atgagaaaca ggaaacattt attgtaccga
     7021 tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatcc atcccttcac
     7081 acaacctggc tctctctttt ttcagataag actgctgggc gatctgccga cgccaggata
     7141 taaccattaa caagcgtcgg cagattggtc atttcttatt tttcgtactg agaactgtcc
     7201 atctactccg gctttcctgg ttaaggcaat ggtttctaag cgatacgcat tattgcaatg
     7261 gtgacagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
     7321 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc
     7381 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
     7441 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
     7501 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
     7561 aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
     7621 gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg ccgactccga ttgttccgca
     7681 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
     7741 gataattttt ctgcggctta cgatctcaac cgggtatttt taatcggcag tgataacctc
     7801 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagcg
     7861 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
     7921 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
     7981 tatctggcga accggttgca ggcgcttttg ggtgtcgagc agaccatccg gatggaaaac
     8041 tttttcttac cgggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
     8101 ctgatttcgc ttaccggacc agaaagtaaa attaagggcg atcctcgctg gatgcaggaa
     8161 cgctcctggt ttggctatac ggaagggttc cgggagctgg tgctgaagaa aaatctgcca
     8221 ccctcatcgc taagcatcgt gtattcggtg ccggttgata aggtgctgga acgcattcgc
     8281 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
     8341 gcacggatgt acgagcgacg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
     8401 gaacatgagc agttcaatcg caagattgtc gcctccgcgc cagtgggtat ctgcattttg
     8461 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
     8521 cttacgcatg aggaccgcca acgactgacg caaattatct gtgggcagca ggtcaatttt
     8581 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
     8641 cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa
     8701 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
     8761 cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
     8821 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcggc tggtgacggc aatgaacaac
     8881 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa gattgaatcg
     8941 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
     9001 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
     9061 gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
     9121 ctgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt tcgcgctgat
     9181 ggcgattatc tctctatccg tgttcgcgat accggcgtgg gcattccggc gaaagaagtg
     9241 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgtaa cttccagggg
     9301 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg
     9361 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
     9421 cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta aacgctgctg gctggcggtc
     9481 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgtt
     9541 acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga cgaggtagtg
     9601 agtaaaaaat ggcagggtag agcggtagtg accttctgtc gtcgccatat tggtattccg
     9661 ctggagaaag cgccagggga gtgggtacac agtgtggctg ctccgcatga gctaccggca
     9721 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
     9781 tcgacggaca aagcggtcag cgataacgac gatatgatga ttctggtcgt ggatgatcat
     9841 ccgattaacc gacgtctgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
     9901 gcgaatgatg gcgtcgatgc gcttaatgta cttagcaaga atcatattga tatcgtgctt
     9961 agcgacgtca acatgccaaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    10021 ggactgacgt tgccggtaat cggagtaact gctaatgcgt tggctgaaga gaagcagcgg
    10081 tgtctggagt ccggtatgga cagctgcctg tcgaagccgg taacgctgga tgtgataaaa
    10141 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggacggataa
    10201 ggcgtttacg ccgcatccgg cattcagtgc aaatgccaga taagacacta acgcgtctta
    10261 tctggcctac aggtgattag tctttatctg ccggacttaa cgtcacagaa gagagataat
    10321 tcagcagggc gatatcgttc tcgacaccca gcttcatcat cgcagatttc ttctggctac
    10381 tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg aagccttccg
    10441 caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcaccg taaccaccag
    10501 cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc cctttctgca
    10561 gcgcggcgag agctttcggc agatcggtcg gtgcaccttg tttcagcacg atcccttcga
    10621 tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc agaacaataa
    10681 tcgacaggct tgggaaatgg cgcttgatgt acttgattaa ggtaatgcca tcgccgtact
    10741 tatcgccagg catggagaga tcggtaatca acacatgcgc atccagtttc ggcaggttgt
    10801 tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca atttgctcaa
    10861 gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt acgttcatat
    10921 tgttcatgta ataggctacc ttgctacagc aagctcttga cataactgtc aatgtcgctg
    10981 atgtattttt ctattcctgg aacatccttc tcacgaatca gatgttccag cgtttcacat
    11041 aactgcttgc cgggtaccag atttagcatg gcaaatacgc ctttaagacg atgagccgtt
    11101 tgggctaacg cagcaaagtc actggttgct gcttcagtat acagcctctt aacatcatcc
    11161 ggtactgtgt ctacaaagag cgcataatag ccgctggcat ggagttgcgc attttcatct
    11221 ccgcccagag gcgattctgt cacctcttcc tgcgccagtt gcacttcaat taattgtaag
    11281 accgcttcct gcatagcgtt gctcatattg aagttgacgc acaattgacc aggcccaatt
    11341 tcccgtacgc cagactcatc atcgcttaaa agcaagccag aggcagtaag attagacgga
    11401 ttatccgtta aaaagatatc ataatcttga ctaattaatc tttcatcggg tgtgatacag
    11461 gttgcacccc aattttctaa ctggcgagtg acaatattcc gaatttctgc cgaagtaaca
    11521 tccaccatta cgcagacatc atccagtaaa cgctcttctt cctcttcaac ttccgggtca
    11581 gcggcgagca ttttgatatg cacagagtag cgtgtaccaa gcccatcccg cgttttgatg
    11641 tttaaatgac cgcccagttt acgcgccagt tgatcgctca gccagaatgc cagcgggtcc
    11701 gccttgccat agcgatcgtt ttgggtctgg ttgataaacg ggaagtgcaa attatccatt
    11761 tcatgaatac ttacgccttc tccggtgtcc agaatgcgga atgtcaggcg gtcttcagag
    11821 gactcatcct gatcaacctc aagggtgatt tttcccaatt gcgttgaggt cacggcatat
    11881 tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat cgccgcggcg catatcgtgt
    11941 gctttcagat gattgttaat cagcagttgc agacctttac gcttgatggc aggcaacact
    12001 gaaggcacaa cttcatcaat taaatcctgc acggagaaca gcaccgtctc acttttccag
    12061 ctatcatccg caagcatgtt cgctaactgt atttcatcaa ccagccgcac cagcacatct
    12121 gcctgattcg ccagttgttt gctttccggg gcgttgagtt tagccgcgct ctccgccagg
    12181 gactgtgcag gttctttcag cgcatcgcca atgtttttca taaaggtcat ccggccctgc
    12241 tggtttttct catacagacg ctgcgcctgc ttgagtttct tgtttaccag cacttcgcga
    12301 tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga cctggctgcg gaacatgcgg
    12361 atctcataca gctcgttatt gatcgtcgcc tgaataatcc cctgatgctg ttccgccatg
    12421 gtggtgatgt tttgcagatt caaatgcggc agcaaatgat cggcaatttt gttacttatg
    12481 acagtgcggt tcgattcctg atcgtgaacc agcaggccga gcggcagcag tgagactatc
    12541 tcttcattaa ttgcccgtaa aatgcgcaat tcgttattga ccgccgtgct gggtagactt
    12601 tctgtactgc ggctggagaa atggcggaat gtggtatagc caaataacgc cagcgccagc
    12661 aaaccgatgt tcagcagcag tggcagcaga atgttttgca acgtatccag caataaggtg
    12721 ccataaggaa cctgccagac caggcgcata tcggtagagt tgagtgccga ggagatttca
    12781 atcttcgtac tgttaaagtg gatactgacg ctatccgtcc cttctttctc attatcattg
    12841 tttcccgttg ccgtcgcgtc tggctcaagg cggaaactgt ccagcggcat acccggtggg
    12901 atcaaatcat taatcggcag atcaaaagcc acgaccgttg ccagatgtcc tggctggtta
    12961 aaggtagtac gcaaggtaaa gtaatgaccg ttctgccagg ccaggcggcg catgttagaa
    13021 aagctttcgc gttcatcgag ggcgttggcc tgttgcaaca tctctgcacg acgtgaatca
    13081 acaatgtcgc tgacggtcga ttctttaaat ccggaggtga gatctttaag gggtagggtt
    13141 gagatcagca ccagactgtt atcctgacca ttcaggtaat acatcgacca cggtacattt
    13201 tctgcgcccc acaatgtatc cagataagtg gacatccgct gagtcatctc aagcgttgag
    13261 ttgtcgtgag agccaaagat taacgcttcc gttttgcggc gtggtttctc cagatagtag
    13321 acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg aggaggggga ggtcgtcgcg
    13381 gcaatgttgt cgtagatctg ccaggtcacg taacgccagg tatcgacgcg cttttggata
    13441 gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca gccatgcgtt aacggcgctt
    13501 tgtaccatta cacccatcgt caccagtaac acaatgatca acagtaaaaa gaagcgggta
    13561 atgctccccg gtaggagtga aaagcgggtc gtggccgttg tctctttctg acgcattcgt
    13621 gtttatgacc tgttaaaact tcgcgaagtg atagggcagt ataaagggta cagtgtgaaa
    13681 ttccagactc ttacctgatt gatgattctc tattctcccg tgatatttat catcctgcat
    13741 tgggggaaat agatgtacaa gcgccatttt gctgtacaaa taacctacaa aaagcccaac
    13801 gaaaataatt gcaaataaac aaagattgct ggaaattatg ctgatgttaa ttatttgtga
    13861 aatagttaac aagcgttata gtttttctgt ggtagcaaag aataatgaaa agtgtgtaaa
    13921 gaagggtaaa aaaaaccgaa tgcgaggcat ccggttgaaa taggggtaaa cagacattca
    13981 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagttatt ctagttgcga
    14041 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
    14101 aaccttgaat tattattgct tgatgttagg tgcttatttc gccattccgc aataatctta
    14161 aaaagttccc ttgcatt
//
LOCUS       DAZFDP010000127        14051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFDP010000127 DAZFDP010000000
VERSION     DAZFDP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHJ1G_003786"
     CDS             complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHJ1G_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4765561.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHJ1G_003787"
     CDS             complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHJ1G_003787"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4765562.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHJ1G_003788"
     CDS             complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHJ1G_003788"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4765563.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHJ1G_003789"
     CDS             complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHJ1G_003789"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4765564.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5254..5373)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5309..5374)
                     /gene="thrL"
                     /locus_tag="ACHJ1G_003790"
     CDS             complement(5309..5374)
                     /gene="thrL"
                     /locus_tag="ACHJ1G_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4765565.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(5588..6274)
                     /gene="yjtD"
                     /locus_tag="ACHJ1G_003791"
     CDS             complement(5588..6274)
                     /gene="yjtD"
                     /locus_tag="ACHJ1G_003791"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4765566.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6674..6814)
                     /gene="yjjY"
                     /locus_tag="ACHJ1G_003792"
     CDS             complement(6674..6814)
                     /gene="yjjY"
                     /locus_tag="ACHJ1G_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4765567.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6910..7626
                     /gene="arcA"
                     /locus_tag="ACHJ1G_003793"
     CDS             6910..7626
                     /gene="arcA"
                     /locus_tag="ACHJ1G_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4765568.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7686..9038)
                     /gene="creD"
                     /locus_tag="ACHJ1G_003794"
     CDS             complement(7686..9038)
                     /gene="creD"
                     /locus_tag="ACHJ1G_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4765569.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9096..10520)
                     /gene="creC"
                     /locus_tag="ACHJ1G_003795"
     CDS             complement(9096..10520)
                     /gene="creC"
                     /locus_tag="ACHJ1G_003795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4765570.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10520..11209)
                     /gene="creB"
                     /locus_tag="ACHJ1G_003796"
     CDS             complement(10520..11209)
                     /gene="creB"
                     /locus_tag="ACHJ1G_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4765571.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11222..11695)
                     /gene="creA"
                     /locus_tag="ACHJ1G_003797"
     CDS             complement(11222..11695)
                     /gene="creA"
                     /locus_tag="ACHJ1G_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4765572.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11906..12775
                     /gene="robA"
                     /locus_tag="ACHJ1G_003798"
     CDS             11906..12775
                     /gene="robA"
                     /locus_tag="ACHJ1G_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4765573.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12772..13419)
                     /gene="gpmB"
                     /locus_tag="ACHJ1G_003799"
     CDS             complement(12772..13419)
                     /gene="gpmB"
                     /locus_tag="ACHJ1G_003799"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4765574.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13471..13992
                     /gene="yjjX"
                     /locus_tag="ACHJ1G_003800"
     CDS             13471..13992
                     /gene="yjjX"
                     /locus_tag="ACHJ1G_003800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4765575.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         3436 a         3839 c         3401 g         3375 t
ORIGIN      
        1 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
       61 accgccgtga tgatcatgat gagctttctt atggtgacgc ggcggtggcg gcggtccgtg
      121 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
      181 gtgaccgcca tcccagtaat aaccgcgatt atcacgatcg cctatctgta attttactga
      241 cgggactaac gtaatttccg cagcctgcgc tgccatggga gcgaccagaa ccagggaaag
      301 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
      361 ggtcgatatc cggagattac gcgatgagaa cggcggttgt tattctccgc aatcctaatc
      421 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgtttct ctccatattt
      481 tcttcacaaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag
      541 atgttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
      601 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
      661 gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
      721 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
      781 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
      841 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg
      901 cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
      961 gggggaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     1021 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     1081 gaagtttccg cttggtaccg aaacaaccag ctggttgcgc gcctcctgcg gcagctgcgc
     1141 aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc
     1201 tgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct tcaccagcgc
     1261 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaatgtaca
     1321 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     1381 attcggcaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     1441 cagaatggtc actggcttat cacccgcaat atgggtcagc atttgtgcca taaagcgacc
     1501 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     1561 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     1621 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     1681 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     1741 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     1801 gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     1861 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     1921 gtcggcaacg cgctgggcgg tatccggctt gtcacacaga gcgaacaagg tcgggccgga
     1981 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     2041 gcctggcagt aatcgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc
     2101 aagctcaggc tgacgggaat agcaggcgtg aatgaagcct gccagatgtc gcccgtgcgc
     2161 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg ccgtcgagac
     2221 tttaatcccc ggatacgcca gcacccacag ccactcatca aacccaggca cttgctggct
     2281 gatgatgtcg ttttcttcga tcatcaactg cataccacct agaaaacacg gtgccacgtt
     2341 gtcgtaatga atgctgccgg agatacgtcc ttccagctcg cccatcaaag ccagcaaacg
     2401 agtgtcatta agcggcttgc cgcagtgttc attcatcgcc atcagcgccg cgaccaccga
     2461 acaggcgctg gagcctaagc ccgaaccgat cggcatattc ttttccagag tcatcgccac
     2521 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     2581 ccgtggttct gacggcagct tatcggcaaa gcgcccgagg ttgttgagac tgaatatctc
     2641 tgccgcctca accgtaacta catctccgag caatgcacca tcaacgggtg tcaccgccgc
     2701 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     2761 catgtcagac tcctaacttc catgagaggg tacgcagcag atcggcaaag acaccagcag
     2821 cggtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     2881 ggctatagaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     2941 ccacttcggc aatcttcacg cgacagacgc catcttcatc aatattgcca acatagcgca
     3001 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagcgatcg tcgagctgtg
     3061 acagattcgc cataaaagcg gcaacatcac cctcggcgtt aaactctgcg ggcagcacag
     3121 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgggcg agaatcaata
     3181 gtttacgcgc cacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     3241 tttcccgtgc cagcgtggtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     3301 tataagaaag cgatcctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     3361 gcagattttg caggttttca ataaccggta atccagcccc aacgttggtg tcatagagga
     3421 atttccgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     3481 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaggaag tcggcatatt
     3541 gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt
     3601 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     3661 cctgccagtt ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa
     3721 caccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     3781 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat
     3841 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     3901 ccacgacaga gattgagcgt tcagaagatc cctgagcaat agcgacaatg ttgatattgg
     3961 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtacgc ataccatcac
     4021 ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt
     4081 tcagttccag gtagaactct tcctgcattg cccgttcagc ccgcacacag tcgctttgcg
     4141 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     4201 gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg
     4261 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     4321 cgtcttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     4381 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     4441 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     4501 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     4561 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     4621 caagtaccac cagttcgcct ttttcattac cggcggtgaa gcctgccatc agcaccatgt
     4681 gatcagccgg aatacgactt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     4741 attcgaggta atgccccact gccagtagtt tttcgaccgg atcgataacg gtaacgttgt
     4801 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     4861 tcagcgcagc gttaatgcta tccgggcact gccctaacaa actaatgcca tgcaggacat
     4921 gttttatttg ggcaaattcc tggtcgacga aagttttcaa ttgcgccagc gggaatcccg
     4981 gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat
     5041 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     5101 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     5161 tatcggcaac ccgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     5221 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa aagcccgcac tgtcaggtgc
     5281 gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga
     5341 tggtggtgat aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct gtaattttta
     5401 tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt caataaaatt
     5461 ttaatttact cacggcaggt aaccagttca gaagctgctg tcagagactc tttttttaat
     5521 ccacacagag acatattgcc cattgcagtc agaatgaaaa gctgaaaaat acttactaag
     5581 gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc aacattgccg
     5641 tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt tttatgtcat
     5701 ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct tgcagttgat
     5761 gttggtctgc cgttgtgtca cttttcgtcg gttgttgtat taatgttgct aattgatagc
     5821 aatagaccat cactgcctgc cccagattga gcgaaggata atccgccacc atcggcacac
     5881 cagtaagaac gtcagccaac gctaactctt cgttagtcaa cccggaatct tcgcgaccaa
     5941 acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc accagttcaa
     6001 ctggcgtggc gtagtaatga tatttcgccc gactgcgcgc agtggtggcg acagtgaaat
     6061 cgacatcgtg taacgattca accagtgtcg ggaaaacttt aatattatca ataatatcac
     6121 cagatccatg tgcgacccag cgagtggctg gctccagatg tgcctgacta tcgacaatcc
     6181 gcagttcgct aaaccccatc gttttcattg cccgcgccgc tgccccaata ttttctgctc
     6241 tggcgggtgc gaccagaata atcgttatac gcatattgcc actcttcttg accaaatgac
     6301 cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa tccttaacgt
     6361 aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc ctgactgtac
     6421 taacggttga attgttaaaa aatgctacat atccttctgt ttacttagga taattttata
     6481 aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg caactagttg
     6541 gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt
     6601 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc
     6661 ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg
     6721 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
     6781 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
     6841 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
     6901 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
     6961 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
     7021 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
     7081 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
     7141 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
     7201 gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg
     7261 ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
     7321 aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag
     7381 cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc
     7441 aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccacac
     7501 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     7561 acgccggaaa tcatcgccac catccacggt gaaggttatc gcttctgcgg tgatctggaa
     7621 gattaatcgg cgttaccacc gtcaaaaaaa atggcgcttt ttagcgccgt ttttattttt
     7681 caaccttatt tccagatacg taattgatcg tccattgtaa cttctttact ggctttcatt
     7741 ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat gccggccagc
     7801 gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc
     7861 cacatcacac catccagcaa caacagcgcg agggtaaaca acatgctgtt gcgccagcct
     7921 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
     7981 catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata aaacatcacc
     8041 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
     8101 aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt agtcgcccgg
     8161 tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt
     8221 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
     8281 ctttgccatt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg caaaaaatca
     8341 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca
     8401 ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc
     8461 tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
     8521 ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggcgcttt caccacacca
     8581 ataccacgcg catccccgac gctaatcaca ataaagggct tgcctaagat gatatttggc
     8641 gcgttgagct cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     8701 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
     8761 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
     8821 tcttcctgca ccgtataaag ctcggtcaca ggaatagcga taagcggccc aacgagtttt
     8881 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt
     8941 tcgacaatca cttgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     9001 aaggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca
     9061 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga
     9121 agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
     9181 cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
     9241 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg
     9301 gtatccagca ccttaagggc gacgtgttcc tgatccactt cggcgcttag cgttatgcga
     9361 ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgctccagt
     9421 aacgccggtt cagcagcaac attaacctca gtgggcataa catgtagagt gatgtttttt
     9481 tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca
     9541 acagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttctacc
     9601 aatgcctgca tacgcgcatt ttgcgtcaga atgttgtcgg taaaacgagc caccacttcc
     9661 ggtggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
     9721 agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
     9781 cgcatacttt ccagcgcctg cgcaagctta cgcaactcgc tactgccgag ttcaggaaga
     9841 ggaacgggct tattgtcagt gacggaatcg gcatagcgag tgagccgggc aatggagcgg
     9901 ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccagcaa aatggcgctg
     9961 gcccataaaa ttcgccgctc gctacgctta atgaccggag ccatcgccgc gttcggtttg
    10021 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
    10081 accgaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata ctgaccacgc
    10141 aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt atttgccgaa
    10201 tcgaacaata ctttaccctg cgaatcggtc atatagacat gatattcatt gcgcactttg
    10261 ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc
    10321 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
    10381 gttgcggtat cgattaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
    10441 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcagc
    10501 aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat
    10561 taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt ttaatgtggg
    10621 tatcaatggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc attagttgct
    10681 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
    10741 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
    10801 aatgaccaat acggatgacg ggagacggcg acgagaactt cttcacccga cgcagtaagg
    10861 tgcgcaccct tgcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc
    10921 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
    10981 gtaacgccgg atggagcgcc agtaactggc ggcataattc aaagccgctg atatcaggca
    11041 aaccaacatc gagaatcatg acgtcgggag cctgctggcg agctttatcc agcaccggca
    11101 agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
    11161 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
    11221 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
    11281 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca taaaagcgca
    11341 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
    11401 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatag
    11461 ccgcatcgga ggtatcttcc gccagaccca atcccccttt aatacccccg gttttcgccc
    11521 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
    11581 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctcc tctgcatgag
    11641 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
    11701 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
    11761 gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta ctaagaaaaa
    11821 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
    11881 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
    11941 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
    12001 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
    12061 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
    12121 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
    12181 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
    12241 tggtattcgc ccgccgctac gtctgggtga attcactatg ccagagcaca aatttgtcac
    12301 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
    12361 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
    12421 gaccattccg ccggtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
    12481 cgaacaagaa gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
    12541 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
    12601 gggaaccggc gtgcaggagt ttatcctgac ggtatatgga acatgcatgc caatgcttaa
    12661 cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag atgccaaagc
    12721 aggagatcgc ccaattaatc tgcgctgtga actgctgatt ccgatccgtc gttaacgctg
    12781 caactcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacaaccca
    12841 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
    12901 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca ggcatcccag
    12961 tgcaatacca tgactcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
    13021 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
    13081 gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc
    13141 gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat caaagatgat
    13201 gtcgcagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct
    13261 actgatgata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
    13321 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
    13381 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
    13441 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat
    13501 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
    13561 catattgcat ccgttgccgt cgagagcggt gtaccggaac aaccctttgg cagtgaggaa
    13621 acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc agaggctgat
    13681 ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg ggtggtgatt
    13741 gaaaacgcca gccagcgcgg cgaggcgcgt tcggctactt tgccgttgcc tgcggtgatt
    13801 ctggaaaaag tgcgtgaagg cgaggcgctc ggccccgtga tgtcgcgtta taccggtatt
    13861 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcacccgc
    13921 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa tgccgtgtat
    13981 caggcattgt aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct
    14041 gatgcgacgc t
//
LOCUS       DAZFDP010000128        14019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFDP010000128 DAZFDP010000000
VERSION     DAZFDP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(2..1360)
                     /gene="plaP"
                     /locus_tag="ACHJ1G_003801"
     CDS             complement(2..1360)
                     /gene="plaP"
                     /locus_tag="ACHJ1G_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4765576.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1350..1412)
                     /gene="yoeI"
                     /locus_tag="ACHJ1G_003802"
     CDS             complement(1350..1412)
                     /gene="yoeI"
                     /locus_tag="ACHJ1G_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4765577.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1627..2556)
                     /gene="yeeY"
                     /locus_tag="ACHJ1G_003803"
     CDS             complement(1627..2556)
                     /gene="yeeY"
                     /locus_tag="ACHJ1G_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4765578.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2602..3426)
                     /gene="yeeZ"
                     /locus_tag="ACHJ1G_003804"
     CDS             complement(2602..3426)
                     /gene="yeeZ"
                     /locus_tag="ACHJ1G_003804"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4765579.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3509..3763)
                     /gene="yoeB"
                     /locus_tag="ACHJ1G_003805"
     CDS             complement(3509..3763)
                     /gene="yoeB"
                     /locus_tag="ACHJ1G_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO4765580.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3760..4011)
                     /gene="yefM"
                     /locus_tag="ACHJ1G_003806"
     CDS             complement(3760..4011)
                     /gene="yefM"
                     /locus_tag="ACHJ1G_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO4765581.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4294..4341
                     /gene="hisL"
                     /locus_tag="ACHJ1G_003807"
     CDS             4294..4341
                     /gene="hisL"
                     /locus_tag="ACHJ1G_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4765582.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4317..4440
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4487..5386
                     /gene="hisG"
                     /locus_tag="ACHJ1G_003808"
     CDS             4487..5386
                     /gene="hisG"
                     /locus_tag="ACHJ1G_003808"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4765583.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5392..6696
                     /gene="hisD"
                     /locus_tag="ACHJ1G_003809"
     CDS             5392..6696
                     /gene="hisD"
                     /locus_tag="ACHJ1G_003809"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4765584.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6693..7763
                     /gene="hisC"
                     /locus_tag="ACHJ1G_003810"
     CDS             6693..7763
                     /gene="hisC"
                     /locus_tag="ACHJ1G_003810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4765585.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7763..8830
                     /gene="hisB"
                     /locus_tag="ACHJ1G_003811"
     CDS             7763..8830
                     /gene="hisB"
                     /locus_tag="ACHJ1G_003811"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4765586.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8830..9420
                     /gene="hisH"
                     /locus_tag="ACHJ1G_003812"
     CDS             8830..9420
                     /gene="hisH"
                     /locus_tag="ACHJ1G_003812"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4765587.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9420..10157
                     /gene="hisA"
                     /locus_tag="ACHJ1G_003813"
     CDS             9420..10157
                     /gene="hisA"
                     /locus_tag="ACHJ1G_003813"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4765588.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10139..10915
                     /gene="hisF"
                     /locus_tag="ACHJ1G_003814"
     CDS             10139..10915
                     /gene="hisF"
                     /locus_tag="ACHJ1G_003814"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4765589.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10909..11520
                     /gene="hisIE"
                     /locus_tag="ACHJ1G_003815"
     CDS             10909..11520
                     /gene="hisIE"
                     /locus_tag="ACHJ1G_003815"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4765590.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11617..12594)
                     /gene="wzzB"
                     /locus_tag="ACHJ1G_003816"
     CDS             complement(11617..12594)
                     /gene="wzzB"
                     /locus_tag="ACHJ1G_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4765591.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12741..13907)
                     /gene="ugd"
                     /locus_tag="ACHJ1G_003817"
     CDS             complement(12741..13907)
                     /gene="ugd"
                     /locus_tag="ACHJ1G_003817"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4765592.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNHFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3537 a         3616 c         3568 g         3298 t
ORIGIN      
        1 attatgctac gtcttcgtac tgcggaaccg gattacggaa actcttggtt acgcaagcca
       61 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
      121 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
      181 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgaaata accgacaggt
      241 taacgaaggt gaacgccacc agcgcaccaa agttaatcag cgccgtagcc attaccaggt
      301 cgaagttgat tgccagcaag gcaatcgcgc caaccaggat gatgttcatt gccggagtac
      361 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacaccgtca cgccccatta
      421 cgtacatcag acgcgctacg cctgcatgcg ccgccatacc ggacgccagt acggtaatgg
      481 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
      541 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
      601 aagttgcaaa gatgaagatc atgccgccaa tcagtgcggt caggaaaatc gcacgcggga
      661 tcacgcgctc tgcatctttg gtttcttccg acaggttgct gatgccgtca aagccggtaa
      721 aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc
      781 agaacggacg agtgctcgcc agcgtacccg cgccttcacc ttcaaatacg ccataaacaa
      841 ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgaagt
      901 tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca
      961 caaacatcca cgatgggatg gaaggcacca gagcttcaaa atagattttc gccagcagaa
     1021 tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga
     1081 cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc
     1141 gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg
     1201 ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca aacagcgtca
     1261 tcggctgcat ataggcaaga cccatcatta caaccggaac taacgtaagc gttttacgca
     1321 attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggtcattctc ctttacggtg
     1381 ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact
     1441 gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg
     1501 tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttagtcatt ttttgacagc
     1561 ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat
     1621 ttttctttaa atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact
     1681 atttgctaaa atttcgtccc gccaccaacc actggcaagc cccgcggtct gttcattcca
     1741 gccaatccag acgggttcaa agagggtttg ggcgaccact ttcttctcga ttaacgcacc
     1801 actatcgaga aaacgttgcg ccagataacg gggtaaatag ccgcagccga ggccgctaat
     1861 ttgcagctcc agcttggttt taaaatcgaa aacggtaata gcttcttgtt cgtcgagaag
     1921 ttgtgaggcc gtagaagcgc cggcctgtgc agtgtccccc accacaatcg cccgatatcg
     1981 tttaatgata cgacggttta acggttcttc ttccagggct aacggatgat gaggcgcgac
     2041 cgcaaagact tgttccagat cgcccaaccg agagaaacca aactcactgg aagatggtgg
     2101 ctcatgcatc gcgccaacaa taatatccgc tcgcccctga gtcagggcat cccatgaacc
     2161 accgagtaca ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcctc
     2221 aatgagtggc gcaagcagag aaaaagggaa ggtgtcgtcc acgccaatca ccagctcgtt
     2281 ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac
     2341 ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct
     2401 gcgatcaagc aattgaatat taaggtcgct ttccagcttg tgaacggtgt aactcagagc
     2461 ggacggcgtc ttgtagagtt tggccgacgc cgccgcaaaa ctgccttctt tttctaaggc
     2521 atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgtatgcg
     2581 gcgtcctgct ggcgtgatcg tttactccag cggcattacc agcggatcgg gatactggta
     2641 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     2701 cagactattt ctgaactgcg gaggttccag ccccaataaa cgggccatct gcggatagaa
     2761 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     2821 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     2881 ttcaccatca ggggcggttt ttccggcaaa gaagcgtccg ggatgacgtc ccggtccgac
     2941 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     3001 gagttcttct aatactcgtc cactgttggt caccggatta cgcggggtgg tttctttcac
     3061 cgtgccttgc gcgtcgccat agacagatgt cgagctggta aaaataatgc ggggaatacg
     3121 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     3181 gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccatca gggcatccag
     3241 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     3301 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     3361 cgctgaaagt gacatcgcca gcggcatgcc taaccaccct aacccgacaa ttgcgacctt
     3421 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     3481 tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca
     3541 gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga
     3601 aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat ggtgttctgc
     3661 gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt
     3721 acagataatc atcccatgat tcctcagacc agattagttt cactcaataa tgtccttttc
     3781 cgttcctttg cctgatttca ggctatcgat tgagtccatc aatctccggg cgttagcggg
     3841 ggaacgcagt agataagccg tctcttccag cgagttgtat tcttcgagtg acatcagaac
     3901 acaagcctct ccattctgac gagtaatgag gatcggggca tgatcttcaa cggctttcat
     3961 cattgttgcc gacaaattct gacgcgcttc gctgtagcta attgtacgca tgtaaatctc
     4021 ctcttttgta cagttcattg tacaatgatg ggtgttaatt cactatttat taattagttt
     4081 gtagatcaag acattgccag cgagacgaaa aatcagactt tgctcttttt ggtgctgtca
     4141 agttagagga cagtcctctt agccccctcc ttttcccgct cattcattaa acaaatccat
     4201 cgccataaaa tatataaaaa agcccttgct ttctaacgtg aaagtggttt aggttaaaag
     4261 acatcagttg aataaacatt cacagagact tttatgacac gcgttcaatt taaacaccat
     4321 catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc
     4381 cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat
     4441 tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga cagacaacac
     4501 tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattact
     4561 ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa
     4621 catgccgatt gatattctgc gcgtgcgtga cgatgacatt cccggtctgg taatggacgg
     4681 cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg
     4741 cgcccagggt gaagatccgc gctactttac cttgcgtcgt ctggatttcg gcggctgccg
     4801 cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct taaatggtaa
     4861 acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggtatctc
     4921 ttttaaatcc tgcttactga acggctctgt cgaagttgcg ccgcgtgccg gactggcgga
     4981 tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt
     5041 cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa tggaagaatc
     5101 caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgtgatcc aggcgcgcga
     5161 atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca tcgccctgct
     5221 gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gaccaacaac gcgtagcgat
     5281 gcacatggtc agtagcgaaa ccctgttctg ggaaaccatg gaaaaactga aagcgctggg
     5341 tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt
     5401 aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc
     5461 ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct cgataacgtg
     5521 aaagcacgcg gcgatgacgc cctgcgggaa tacagcgcga agtttgataa aaccacggtt
     5581 accgcgctga aggtgtctgc tgaggagatc gccgccgcca gcgaacgcct tagcgacgag
     5641 ctaaaacagg cgatggcggt ggcagtaaaa aatattgaaa ccttccacac tgcgcaaaaa
     5701 ctgccgccgg tagatgttga aacacagcca ggcgtgcgtt gccagcaagt cacgcgcccg
     5761 gtagcttcag ttgggttgta tattcctggc ggctccgccc cactcttctc aacggtatta
     5821 atgctggcaa ctcctgcgcg tattgcgggc tgtaaaaaag tggtgttgtg ctcaccgccg
     5881 ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga cgtgtttaac
     5941 gtcggcggcg cacaggccat tgccgctctg gcgtttggta cggaatctgt gccgaaagtg
     6001 gacaaaatct tcgggccggg taacgccttt gtcaccgaag cgaaacgtca ggtgagccag
     6061 cgtcaggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct ggtgattgct
     6121 gacagcggcg ctacgccaga tttcgtggct tctgatttgc tttctcaggc tgaacacggc
     6181 ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg cgttgccgag
     6241 gccgtcgaac gccagttggc agaactgccg cgtgccgaaa ccgcccgcca ggcactgaac
     6301 gccagccgcc taatcgtgac caacgattta gcacagtgcg tggagatctc caaccagtac
     6361 ggcccggagc acctgatcat tcagacccgc aacgctcgcg atctggtcga tagcatcacc
     6421 agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggtga ttacgcctcc
     6481 ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg
     6541 gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggctt ttctgcgctg
     6601 gcttcaacca tagaaacact ggccgccgcc gagcgcctga ccgcccataa aaatgccgtt
     6661 actttgcgtg ttaacgccct taaggagcaa gcatgagcac cgtgactatt accgatttag
     6721 cgcgtgaaaa cgtccgcaac ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg
     6781 gcgacgtctg gctgaacgcc aacgaatatc ccgcagccgt ggagtttcag cttactcaac
     6841 aaacgctcaa ccgctacccg gaatgccagc cgaaagcggt gattgaaaat tacgcgcaat
     6901 atgcaggcgt aaaaccggag caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac
     6961 tgctgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt
     7021 acggcatgta cagcgtcagc gccgaaacga ttggcgtcga gtgtcgcaca gtgccgacgc
     7081 aggacaactg gcaactggac ttacagggca tttccgacaa gctggacggc gtaaaagtgg
     7141 tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag gattttcgca
     7201 ctctgctgga gctaacccgc ggaaaagcca ttgtggttgc cgatgaagcc tatatagagt
     7261 tttgtccaca ggcatcgctg gctggctggc tggcggaata tccgcacctg gctattttgc
     7321 gcacactatc gaaagctttt gctctggcgg ggcttcgttg cggatttacg ctggcaaacg
     7381 aagaagtcat caacctgctg atgaaagtga tcgcccccta cccgctctcg acgccggttg
     7441 ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgc gaacgggtgg
     7501 cgcaaattat tgcagaacgc gaatacctga ttgccgcact gaaagagatc ccctgcgtgg
     7561 agcaggtttt tgactctgaa accaactaca ttctggcgcg ctttaaagcc tccagtgcag
     7621 tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt
     7681 taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg
     7741 ccttacgtgc ggagcaagtt taatgagtca gaagtatctt tttatcgatc gcgatggaac
     7801 cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga
     7861 accgggcgtg atcccggagc tgctgaagtt gcaaaaagcg ggctacaaac tggtgatgat
     7921 cactaatcag gatggtctgg gaacacaaag tttcccgcag gcggatttcg atggcccgca
     7981 caacctgatg atgcagatct tcacctcgca aggcgtacag tttgatgaag tgctgatttg
     8041 tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac tggtggagcg
     8101 ttatctcgct gagcaagcga tggatcgtgc caatagttat gtgattggcg atcgcgcgac
     8161 cgacattcaa ctggcggaaa acatgggtat taatggttta cgctacgacc gcgaaaccct
     8221 gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc atgtagtgcg
     8281 taacaccaaa gagacgcaaa ttgacgtcca ggtgtggctg gatcgcgaag gtgacagcaa
     8341 gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca cccacggcgg
     8401 tttccgtatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc acacagtcga
     8461 agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc cttggcgaca aacgcggtat
     8521 ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt gcccgctgcg cgctggatat
     8581 ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgcg tgggcgatct
     8641 cagcaccgaa atgatcgagc acttcttccg ttcgctctct tacaccatgg gcgtgacgct
     8701 acacctgaaa accaaaggta aaaacgatca tcaccgtgta gagagcctgt tcaaagcctt
     8761 tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac accctgccct cgtcaaaagg
     8821 agtgctgtaa tgaacgtggt gatccttgat accggctgcg ccaatctgaa ctcggtgaag
     8881 tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cgtcgtgttg
     8941 ctggccgata aactgttttt acccggcgta ggcaccgcac aagcagcgat ggatcaggtg
     9001 cgtgagcgcg aactgtttga tctcatcaaa gcctgtaccc aaccggtgct gggaatctgc
     9061 ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcgtcga cttgctgggc
     9121 atcattgacg aagacgtgcc gaaaatgacc gactttgctc tgccactgcc acatatgggc
     9181 tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc agggaattga agacggcgcg
     9241 tacttttact ttgttcacag ttacgcaatg ccggtcaatc cgtggaccat cgcccagtgt
     9301 aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc
     9361 cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga
     9421 tgattattcc ggcattagat ttaatcgacg gcactgtggt gcgtctccat cagggcgatt
     9481 acggcaaaca gcgcgattat ggtaacgacc cgctgccgcg attgcaggat tacgccgcgc
     9541 agggtgcaga attgttgcat ctggtggatc tgaccggggc aaaagatccg gctaaacgtc
     9601 aaatcccgct gattaaaacc ctggtggcgg gcgttaacgt gccggtgcag gttggcggtg
     9661 gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc aggcgttgcg cgcgtggtgg
     9721 ttggctccac cgcggtgaaa tcaccagaga tggtgaaagg ctggtttgaa cgtttcggtg
     9781 ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg
     9841 cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg gaaagctatc
     9901 tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc acgctggcag
     9961 gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg gcgtttcagt
    10021 cctccggcgg tattggcgac attgatgatg tggcggccct gcgtggtact ggcgtgcgcg
    10081 gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag gccatcgcat
    10141 gctggcaaaa cgcataatac cgtgtctcga cgtccgtgat ggtcaggtgg tgaaaggcgt
    10201 acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac gctacgctga
    10261 agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt
    10321 agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct gcgtggcggg
    10381 tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc
    10441 catcaactcc cctgcgctgg cagacccaac attaattact cgcctggcgg atcgttttgg
    10501 cgtgcagtgt attgtggtgg gtattgatac ctggtacgac gctgaaaccg gtaaatatca
    10561 tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg aaacactcga
    10621 ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc gtcctcaaca tgatgaatca
    10681 ggacggcgtg cgtaacggtt acgacctcga gcagctgaaa aaagtgcgag aagtttgccg
    10741 cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctcg aagccttccg
    10801 tgatgccgac gttgacggcg cgctggcggc ttccgtattc cacaaacaaa taatcaatat
    10861 tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa
    10921 caacaacgtc gcgaactgga ctgggaaaaa accgacggac tgatgccggt gattgtgcaa
    10981 cacgcggtat ctggcgaagt gctaatgctg ggctatatga acccggaagc cttagacaaa
    11041 accatcgaaa gcggcaaagt cactttcttc tcgcgcacta aacagcgact gtggaccaaa
    11101 ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg cgccggactg cgacaacgac
    11161 acgctactgg tactggcgaa tcccatcggg ccaacctgcc acaaaggcac cagcagctgc
    11221 ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaatt gctcgccgaa
    11281 cgcaaatctg ccgatccgga aacctcttac acagccaaac tgtatgccag cggcaccaaa
    11341 cgcattgcac agaaagtggg cgaagagggc gtggaaaccg cactagcagc aacggtacat
    11401 gaccgctttg aactcaccaa cgaggcgtct gacttgatgt atcacctgtt ggtgttgttg
    11461 caggatcagg atctggattt aacaacggtg attgagaacc tgcgtaaacg gcatcagtga
    11521 gttgcggggt aagcggatgc gatgttgttg tcgcatccgg caaaaaaaac gggcaaggtg
    11581 tcaccaccct gcccgttttc tttaaaacca aaaagattac ttcgcgttgt aattacgcag
    11641 agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca ccgccagaat
    11701 caaagtgatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt tcatcacata
    11761 gcggtaagca tgaatatcaa gatcatcaac ttttaagctt tcgatatcca gcaggttttg
    11821 acgtgtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat gcttaatcat
    11881 cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat cttcagtctg
    11941 ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt cctgaatctg
    12001 acggatacgc agatctttct gctcctgcgc aactacttcc tgagttttca gagaatcctg
    12061 caagtttttc atacgcagcg cgatgttgtc tttcaggtct tttcccagtt cttcatttac
    12121 ctggtcgtca acttgctgga tatacttcgc cagctgcttc tgagcacctt caggtgtttc
    12181 accaacataa gacaccgcta aaggtaatga ctgatttttg acggtaggct caatggtcag
    12241 cttttctgct tcttcctggt tatccagcgt ttccgctaat gctgagaatg cagtgctgta
    12301 acgtccgata agtgaagcct gaatgtcgga gactttcggt acagccgatc cgtagatcac
    12361 attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa cgatagctgt
    12421 ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa tagcaacaat
    12481 gacagagatg ataattgtca tcttgccacg ccacaactgc accagtaaat caatcaaatc
    12541 aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc tcatcattac
    12601 cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca tgaatgctat
    12661 aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat cagggcgaaa
    12721 atcgccctga taacaggatg ttagtcactg ccaaagagat cgcgggtgta gactttgtct
    12781 gccacatcct taagctcttc tgccatacgg ttggagataa ttacgtcggc ttgttgtttg
    12841 aaagtggcga gatcacgttc cagccgagag ttgaagaatg agtcttcttt catcactggc
    12901 tcgtagatga ttacttcaac acctttcgcc ttgatacgct tcataatccc ctgaatagaa
    12961 gacgcacgga agttatctga accgctcttc ataatcaggc gataaatacc caccacatgc
    13021 ggtttgcgtg acaaaatggc atcggcaata aaatctttac gcgtgcggtt tgcatcgaca
    13081 attgccgaga tcaggttatt cggcacagac tgatagttcg ccagtaactg cttggtatct
    13141 ttcggcagac aataaccacc ataaccaaac gacggattgt tgtaatggtt gccaatacgc
    13201 ggatcgagac aaacgccttc gataatctgg cgagtattca gacctaaact ttctgcgtag
    13261 ctatccagtt cattaaagta cgctacgcgc atcgccagat aggtattagc gaaaagttta
    13321 atcgcttctg cttcggttga gtcggtaaac agggtcggga tattttgctt aatcgcgcct
    13381 tcctgtaaca gagcagcaaa acgttctgcg cgttctgaac gctcaccgat gacaatacgt
    13441 gaaggatgga gattatcgta aagggcttta ccctcacgga gaaattccgg agagaaaata
    13501 atatttttag tacgatattt cttatgcatc gctgcggtaa agccaacggg taccgttgat
    13561 ttgatgacca taaccgcata aggatttatc tctactacgt ccttaattac tgattctaca
    13621 ctcgatgtat tgaaatgatt agtgttagga tcatagtcgg ttggagtggc gatgatgaca
    13681 taatcagcat cccggtaggc ttcattttta tctaatgtgg cattaaagtg tattttatct
    13741 gattgcaaaa actgctgaat ttccttatca acaataggag atatccgatc attcagcata
    13801 gcaacgcgtg acggtaaaat atctaatgcc acaacctcat gattttgtgc gattagaagc
    13861 ccgtttgaca agcctacata gccggtaccg gaaatggtga ttttcatttc atgctctcag
    13921 aattaactta actgtgaatc atgatgtttt tagcatcctg ataagagcta aaagttttaa
    13981 cgctacgttg ttcgatggtc aacgcaacga aaaacaatt
//
LOCUS       DAZFDP010000129        13240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFDP010000129 DAZFDP010000000
VERSION     DAZFDP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..216)
                     /locus_tag="ACHJ1G_003818"
     CDS             complement(<1..216)
                     /locus_tag="ACHJ1G_003818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765593.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHJ1G_003819"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHJ1G_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO4765594.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHJ1G_003820"
     CDS             complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHJ1G_003820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4765595.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            2567..3280
                     /gene="yghS"
                     /locus_tag="ACHJ1G_003821"
     CDS             2567..3280
                     /gene="yghS"
                     /locus_tag="ACHJ1G_003821"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4765596.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3312..4070
                     /locus_tag="ACHJ1G_003822"
     CDS             3312..4070
                     /locus_tag="ACHJ1G_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765597.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4116..5465
                     /locus_tag="ACHJ1G_003823"
     CDS             4116..5465
                     /locus_tag="ACHJ1G_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO4765598.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5465..6289
                     /locus_tag="ACHJ1G_003824"
     CDS             5465..6289
                     /locus_tag="ACHJ1G_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765599.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6301..6861
                     /locus_tag="ACHJ1G_003825"
     CDS             6301..6861
                     /locus_tag="ACHJ1G_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4765600.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6892..7962
                     /gene="lptF"
                     /locus_tag="ACHJ1G_003826"
     CDS             6892..7962
                     /gene="lptF"
                     /locus_tag="ACHJ1G_003826"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4765601.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7959..9038
                     /gene="lptG"
                     /locus_tag="ACHJ1G_003827"
     CDS             7959..9038
                     /gene="lptG"
                     /locus_tag="ACHJ1G_003827"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4765602.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9074..10246)
                     /locus_tag="ACHJ1G_003828"
     CDS             complement(9074..10246)
                     /locus_tag="ACHJ1G_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4765603.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10246..10494)
                     /locus_tag="ACHJ1G_003829"
     CDS             complement(10246..10494)
                     /locus_tag="ACHJ1G_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4765604.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10526..11440)
                     /locus_tag="ACHJ1G_003830"
     CDS             complement(10526..11440)
                     /locus_tag="ACHJ1G_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4765605.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11437..13125)
                     /locus_tag="ACHJ1G_003831"
     CDS             complement(11437..13125)
                     /locus_tag="ACHJ1G_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO4765606.1"
                     /translation="MRYADFPTLVDALDYAARSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3179 a         3262 c         3438 g         3361 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg aatatggggt
      421 caacccacgg tctggcgatg gtcttttcca tgctgctggc ggcgattata tggaacctgg
      481 ggacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggc gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggtggtct gattttcctg ctgcgccgtt tctggagcgg gacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 ggcagaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac tcgcgatgcc gtctccaact
      961 tcgaacacta tctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc
     1021 cattgcctgc ggcatcgact gatggcgcgc aagtaacaga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctcagata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggtctt gccagttata ttgggatgcc cgtctccaca acacacgtcc
     1501 tctcgtctgc agttgcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgggtattta ctttaccggc ggcgattttt ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga
     1681 tcgggcattt ccatttttgg ctagtgataa ccacgcgcgg tcataaaatc cataatcgct
     1741 ttttctgcat caaccaacac ctgttccagt ggctgattgg catcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gctatctttc ttgccagcga ctcccttttg
     1861 tggtcaggct tacgcgcgca ggcaacttca aggtcaacat tgagtttgat gaccagatca
     1921 ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg
     2041 tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcct
     2161 ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa ggattttccc
     2221 atcaacggta attttgtcac tgcacgaccg acatttccgg cttgttttcc taaatgaact
     2281 ctttcggcag caccatattt ttcgacaacg gtaataagat gttcacacac cgttgacttg
     2341 cctgaaccat cgctaccaat aacggcaatt aatggaggtg ttattgactg catgaatata
     2401 tccttaaatt taaatatcca tccaaaatat ttatttggtt aatatgtttt tatgaaagcg
     2461 taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg atattctgag
     2521 cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga atcaacgtaa
     2581 tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat gtgatggttc
     2641 aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccaacaga
     2701 acgcatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctccctgt
     2761 tattggcgca ccttttgggc gttatctgcg aagtaaagcg gcacatgtgc acgaaaagcc
     2821 ctcaacaccg cctggcaata ttactgcgct ggttatctat ctgctttcct gctggcgggc
     2881 gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg
     2941 ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg caaaaaccac
     3001 gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt accaatggat
     3061 ggcatcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc
     3121 gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact
     3181 gacattcaat ggcgcaaaga tccttgaact ggatgggcga caccccgccg atgaaattct
     3241 gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga
     3301 tgatttaacg aatggatgca ctacaaactc aaactgttaa tagtacaacc gcgccgcagc
     3361 ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt aaatccacac
     3421 tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg cgctatctgg
     3481 ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc
     3541 tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg
     3601 cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca
     3661 aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg
     3721 aaatttcggg cttttattat gatggaccgg ggattggcgt cgaacgtgcg accgggaaaa
     3781 tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg caatatcgcc
     3841 cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc aagcctgacc
     3901 atgactatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc tataacggca
     3961 caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa caggcacaga
     4021 aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg
     4081 atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt
     4141 ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta
     4201 atgtcgtgag cgccttgctg ggtctgttgg cgctctcgtg tgccggtaaa gggatgacgc
     4261 ccgccatgtt tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc agcgatttta
     4321 ttaagtttca gacatggcaa ctggtggttc agtacggaac gccagccttg accaacaata
     4381 atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg
     4441 cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca ttaggtctgg
     4501 atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca atggcttcct
     4561 ccacgccgac cggcattctg cgtgcggtag atcgcttcga tttaattgct gtacagcagg
     4621 cgacgaaacc ttttctgcgc gcagcgggga gcgtcgtagc ctggtatttt gactttggtt
     4681 ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt
     4741 ggtttgcagc gcgcgaatta cgccgcagaa atatccataa tgccttcaaa ttgaatctgt
     4801 ttgagtctgc ccgacacatc aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc
     4861 actccatctg gtcggcgcgt aactcgtgca gcacagtgct ggtggggatc gtgttaggac
     4921 ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag
     4981 cgggtttgct gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca
     5041 gaccgtggtt gttaggtgtg aagtccggtt tactggcggg cggaatcggt attctggtgg
     5101 cgctcgcggt gtttatcgtc ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc
     5161 tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg atgctgggct
     5221 tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa
     5281 ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc
     5341 tgggagcagc gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc
     5401 caaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat
     5461 cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga
     5521 agaagatgat gtcgttgatg cacaggttag tttgccgccg actattgcac taagttgtac
     5581 caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga
     5641 tcgtcaggaa ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc tgactgctga
     5701 tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg
     5761 gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg tttttctggg
     5821 gcgttttcag gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct
     5881 gttacggatt tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag
     5941 ccagttagaa agcgtaagca gttttattgc ctatcggcaa aagcaaatgc ataccctgaa
     6001 agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat
     6061 cagccaacag gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc
     6121 gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggagata aacacgacga
     6181 catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccgttgg cgctggatag
     6241 cgatattcgt cagcgcttag agctgctgat ttcgcgtttt ccgctatagc cggagcaatg
     6301 atgtcatctc gtttgattat tgcgttgatt atcatgctct tagccccggg tgtgcaggca
     6361 cacaatttcg tcactggcaa aacggttacc cctgtttata tccaggaagg cggcgagctg
     6421 cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca attagcagga
     6481 aaagtccgca ttattcagta tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg
     6541 ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc caccaccatt
     6601 gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa
     6661 aataaacgcc gctatccgtg ggcgcaattc attattgacg gcaatggaca gggacgcgtt
     6721 gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc cgggcaaatt
     6781 cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgctggcg
     6841 cagaaactga tcaatgaata aaaaattcaa aggattgagg gcgttagtga gatgaagctg
     6901 gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg
     6961 atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca
     7021 ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc actggagatg
     7081 ttgttacctg ttggtctgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgac
     7141 tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt
     7201 ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg
     7261 tgggcttata cgcagattta tcaactggag cagcagtcgc agtcggagct ggatgttcgc
     7321 cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg
     7381 gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac tgccaatcga
     7441 acccgcattt tccgcgcccg ttcggttgat gtggttgacc catcacccga gaaaccgacc
     7501 gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa
     7561 cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc taacgtcaaa
     7621 cgcaaagcaa aatcggtcac ggagctggcg cgctccgcct ttcctgccga tcatgccgaa
     7681 ctgcaatggc gacaaagccg tggcctgaca gcattgttga tggcgctgct ggccatttca
     7741 ttaagtcggg taaaaccgcg gcaagggcga ttttcaacat tattgccact gacgttgctg
     7801 tttattgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt
     7861 cccctcattc ctggtttgtg gttagtaccc ggactcatgc taatgggcct gctgatgctg
     7921 gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt
     7981 taatccgtca tctctttctc ggttttgccg ccgccgcagg gctattgctg ccgcttttta
     8041 ccaccttcaa cctgattaac gaactggatg atgtcagccc gggcggttat cgctggactc
     8101 aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt tcgccgttta
     8161 tcgcgttatt gggtggtatt gttggactgg ggcagttatc gaaaaacagt gagcttaccg
     8221 ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggtgctg gttgccggga
     8281 tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac
     8341 aggcgttgca aatcaaatcg accgccaccg cgttggggga ggacgatgac attaccggca
     8401 atatgctgtg ggccaggcgt ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg
     8461 gccagccggt gggcgtggag atatttcatt atcgcgacga tctctcgctt gaatcctaca
     8521 tttatgcacg cagtgccacc attaaagacg acaaaacgtg ggtcctgcat ggtgtgaatc
     8581 ataaaaaatg gctgaatggc aaagaaacgc tggaaacatt agataatctt gcctggcaat
     8641 cggccttcac cagtatgaat cttgaagagt tatcaatgcc ggggaatact ttttctgtcc
     8701 gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc
     8761 gccttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg
     8821 ctgtgccgtt tacctttact gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag
     8881 gcgtcatcgt gggcttactc acctggatca gctatcaaat catggttaat ctgggattgc
     8941 tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg
     9001 cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc gcaaacacca
     9061 cggcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct
     9121 cacgactgtg ggcagcattt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag
     9181 gcggcaggat aagattcaca tacacgccca gcgagataag ctcgcgccag atgcgtaaac
     9241 cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcat
     9301 aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca
     9361 gctctggttg tgtggcaata gttcttaacg acgagcgcac ggtcgcaatg caggacggag
     9421 aaggagaggc ggtaaaaata tacgggcggc tgctgtagcg cagcacttcc atcgcttccg
     9481 aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaagtacca acgataatat
     9541 ccacatcatc ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg cctaacacgc
     9601 caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa
     9661 tttccgccag cggcgcaacg tcaccgagca tgctgtagat gccctcaacg atgataatcg
     9721 cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat
     9781 ggcggaaacg aataatttcc gcgccaccga gagaacaggc gtcataaata ctggcgtggc
     9841 tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat
     9901 tagcggtata accggttgaa aaaacgatag cggtagggcg attgaaaaac tcggcaatct
     9961 ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc
    10021 cggtgccttg ggccgctaac gctgcctgac cttcgctgat ggcatcatga ttaaaggtta
    10081 aaccgagata attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg
    10141 tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg tcactaaatt
    10201 gcagtcgctc gccagcaagg cgtgcatatt tatcgtatag ccccattatt tattctccag
    10261 ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca gaatattaat
    10321 aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga
    10381 ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac
    10441 caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac
    10501 gttaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc
    10561 taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc gaccagtcgg
    10621 cgtgggttaa ttcacgaatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac
    10681 tcaacgcggt actgatatcc gcaaggcagg tgagtaccgg cagaggaata ccaaccattc
    10741 gcacggaacc gcaacggacg ttggcggcaa gttgctgtat gcgttgccag tcatagccgc
    10801 cagcgacgct atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact
    10861 gacccactgc ttgcgcaaaa tcggtgacgt gcaggaaaga gagctgtgta tctggtgcac
    10921 caagtcgtgg cagcaggccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc
    10981 cgggaccata cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag
    11041 tcagccgttg ttcggcgatg tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg
    11101 ccgccagcga agagataaac agaaaacgtt ggcaaaagca gctctctttt gctgcttgca
    11161 tcaggcgcag gctgccgtca acgttacagc gggtgaaaat ctcttctttg tgcccgcgca
    11221 cttgcccggc gcaatggacc actgcgctgg ctccggcaac cagcttgcta agtgaatgag
    11281 tatcttccag cgaaccgcgc acccaggtga gattatcatt gacgtgagcg cgggcagtac
    11341 gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcgataata tatttaccga
    11401 taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatt
    11461 aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc
    11521 ggaggacgtt cggggaatac tgtggggcgg caacagatcg atagccgcgg tcacgccaaa
    11581 ttcgctttgg atccgtgccg ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct
    11641 gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc
    11701 gccagaatga atttccggtt cttgttccgc aatatattca atatcctgcg gccagatatt
    11761 acggccacga ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag
    11821 cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaaac
    11881 ctggtcgcca aagtaaccgc tcatcaggct ggggccggag atgcaaatat ggcctaccac
    11941 acgttccgcg accggcatac ctgcttcatt gcggatttca atgccatgtt ccggcaacgc
    12001 tttgccgcag ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg cgacggcttt
    12061 accctgatat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aggcttcatc
    12121 agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatt
    12181 gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg cggagatggg
    12241 ttctgcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc
    12301 attcacgcgg cgctggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg
    12361 atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa
    12421 ataatctact gaaagctgcg tggcgacggg gggcaggaga aagccgacca gtcccatatc
    12481 atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg
    12541 gcttatagca cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa
    12601 acgggtgctg ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag
    12661 cgcaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg
    12721 gttgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga taatggctgc
    12781 gggctggcaa cttgccagta aaccctggag tttggcgctc caggaatccc gctgaccgac
    12841 gcccattgga atcgccaacg ggacggcgac taagccagca tactgacagg caaaaaaagc
    12901 ctctacaaac ccgctactcg tttcggcaat cagcgccacg cgttcgcctt ttttcaggtt
    12961 cagcgataac aaccgcttcg caccagcttc ggcacgcgtt tttaatgtct gatattccag
    13021 ttgatcttca agttggcaac gtctgtcata aaaattcatt ccggcgctac tccgagcggc
    13081 gtagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt
    13141 aaagatttta ttagacatat acaccacgaa aaaattggat atcaaaaata aaatgacaat
    13201 aatgcggtgg attatatagg cataaaagat aaatgagata
//
LOCUS       DAZFDP010000130        13202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFDP010000130 DAZFDP010000000
VERSION     DAZFDP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(733..1824)
                     /gene="ychF"
                     /locus_tag="ACHJ1G_003832"
     CDS             complement(733..1824)
                     /gene="ychF"
                     /locus_tag="ACHJ1G_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4765607.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1941..2525)
                     /gene="pth"
                     /locus_tag="ACHJ1G_003833"
     CDS             complement(1941..2525)
                     /gene="pth"
                     /locus_tag="ACHJ1G_003833"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4765608.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2803..3081
                     /gene="ychH"
                     /locus_tag="ACHJ1G_003834"
     CDS             2803..3081
                     /gene="ychH"
                     /locus_tag="ACHJ1G_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4765609.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3136..4815)
                     /gene="dauA"
                     /locus_tag="ACHJ1G_003835"
     CDS             complement(3136..4815)
                     /gene="dauA"
                     /locus_tag="ACHJ1G_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4765610.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4940..5887)
                     /gene="prs"
                     /locus_tag="ACHJ1G_003836"
     CDS             complement(4940..5887)
                     /gene="prs"
                     /locus_tag="ACHJ1G_003836"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4765611.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6038..6889)
                     /gene="ispE"
                     /locus_tag="ACHJ1G_003837"
     CDS             complement(6038..6889)
                     /gene="ispE"
                     /locus_tag="ACHJ1G_003837"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4765612.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6889..7512)
                     /gene="lolB"
                     /locus_tag="ACHJ1G_003838"
     CDS             complement(6889..7512)
                     /gene="lolB"
                     /locus_tag="ACHJ1G_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4765613.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7726..8982
                     /gene="hemA"
                     /locus_tag="ACHJ1G_003839"
     CDS             7726..8982
                     /gene="hemA"
                     /locus_tag="ACHJ1G_003839"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4765614.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9024..10106
                     /gene="prfA"
                     /locus_tag="ACHJ1G_003840"
     CDS             9024..10106
                     /gene="prfA"
                     /locus_tag="ACHJ1G_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4765615.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10106..10939
                     /gene="prmC"
                     /locus_tag="ACHJ1G_003841"
     CDS             10106..10939
                     /gene="prmC"
                     /locus_tag="ACHJ1G_003841"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4765616.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10936..11328
                     /gene="sirB2"
                     /locus_tag="ACHJ1G_003842"
     CDS             10936..11328
                     /gene="sirB2"
                     /locus_tag="ACHJ1G_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4765617.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11332..12141
                     /gene="sirB1"
                     /locus_tag="ACHJ1G_003843"
     CDS             11332..12141
                     /gene="sirB1"
                     /locus_tag="ACHJ1G_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4765618.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12177..13031
                     /gene="kdsA"
                     /locus_tag="ACHJ1G_003844"
     CDS             12177..13031
                     /gene="kdsA"
                     /locus_tag="ACHJ1G_003844"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4765619.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3317 a         3308 c         3395 g         3182 t
ORIGIN      
        1 atgagcttct cagagaataa ttctctatta ccgggcaaaa tcaggaacac aggaattaat
       61 atatatttaa catccccctt acaaaggtga gtttatatcg agttattccc taagttgtcg
      121 ttgcttattc caattcgtta ctataaatat ggcaaaaata ttacaacaaa cagcggttaa
      181 aaaatttggt gaagattttt tgtaataatc ctaattagtt atgggaattt cgcttgatgt
      241 atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa aagcaaaaaa
      301 catcgatatt catttttggt caatgggttt tctggcctat cgttgttatt gtgctactgg
      361 ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac tttatctgcg
      421 ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgtg atcaatattg
      481 atcagctgtg aagggggtga tgtgtagtgg accaaaactt acgttatatc actactaatg
      541 agatatccgt catctgacac tgatgtcttt aattattctc gtggttcact gtaggcaata
      601 agcacaaaag tgtaggatgt tacaagaatg attaggactc ggtgaaatga aaaatccacg
      661 caattgcgtg gattatatat acttttgcgc tcttcatgag atttagcgaa acctcatgag
      721 acaacaaatt aattagacgt tgaaaaggaa gttcatcaca tcgccatctt taacgatgta
      781 atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt
      841 gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa aatcagtatg
      901 gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg cacgcacttc
      961 tttcacccca gcggtgaagt aagtttgcag gttcagcagc ttataaccgg cacggatcac
     1021 acggttcagg cccggctctt ccagcccaag ctcctgcata aactcgtcac gttcttcgtc
     1081 gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa cagaaccttc
     1141 tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt
     1201 gacgttggcg atgtacattg ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc
     1261 tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcattttcca actggggcag
     1321 gcatttttcc aggaccgcca gctcagcttt cgcgtcttta tcgccacctt tggctttctt
     1381 ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt
     1441 gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga tgttgtcatt
     1501 ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg tcaggaactg
     1561 gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc
     1621 catggtcgtg ggaagcgtac gttgcggttt tacgatttca gccagttgat ccaggcgagg
     1681 gtcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc
     1741 ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc
     1801 gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat aatcaacctg
     1861 ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg gcactcggca
     1921 aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg
     1981 gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcgat ggcttcatca
     2041 attaacttct gttcactaac aggcggtttg cctaacacaa aaccgacaac tttattttta
     2101 tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt acccaattta
     2161 ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaattt aaatttggcg
     2221 acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg
     2281 aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt agtcgggact
     2341 aacaggcgga catcttcgcc tccaagagtg actcgcgaag tataaccaaa gaatttagcc
     2401 tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca ggcaccagca
     2461 ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat caatttaatc
     2521 gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg tagtttactg gttgcggccc
     2581 cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa taattgcatg agtggactat
     2641 tagaaagtca aggtgttcag gcgtttattt gtaaagtttt gttgaaataa gggttgtaat
     2701 tgtgatcacg cccgcacata acccacaggg tgttgtctat actttacaca taaggaagag
     2761 gggtattccc tgttacaacc cagaaagttc cggaggtgac atatgaaacg caaaaacgct
     2821 tcgttactcg gtaacgtgct catggggttg ggtctggtgg taatggtggt cggcgtgggg
     2881 tattcaatcc tcaaccagtt accacagttt aatatgcccc agtatttcgc acatggtgca
     2941 gtgctaagta ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt tggcgggcat
     3001 gaacaggtgt gcgaccgtta ctggtgggtt cgccactatg acaaacgttg ccgccgtagc
     3061 gataatcgcc gtcatagcta acaaataatg cagtttgctg accagtcagt ttgcgctgac
     3121 tggtcaatct cgtacttata aatccgccat cgccgcgcga cgattcggga agaacgccag
     3181 gcgtcccggg atcggttgaa tgccagcgcg agccatagtg cgcagtggct ggaattccac
     3241 gttgcacacg cgcagttcac atccctcggg cagacgcttc acaaaacgct ggaacgcatc
     3301 aagaccacca gcatcaagta ccggaaccgc atcccacttc agaatcacaa tccgtttgcc
     3361 ttcaagacgt gactccaggt ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc
     3421 aataacgcgc agaaccagga catcgtctgg aacatctacg actaccggtg ccaggcgagt
     3481 catacgtgcg atacgacgca taaacagcag cgatgccagc acgatcccca cgctgatggc
     3541 aataaccata tcaaacagca cggtcagcga catgcacagc agcatgacaa tgatgtcatc
     3601 tttcggcgca tgacgcagca agtcgaccac tttgtgcgct tcactcatgt tccacgccac
     3661 catcaacagc agggcagcca tggcggaaag cggcagccag gagagcagcg gtgccagtac
     3721 cagcagggca agaataacca gaatagagtg gatcaccgcc gagatagggg aagttgcccc
     3781 ggcacggacg ttagcggcag aacgcgcgat ggcagctgta gcggtaatgc caccaaagaa
     3841 cggagcgatg atattcccca gtccctgtcc aaccagttcg ctattcgcct tgtgtttcgt
     3901 cccggtcata ccatccagca ccacggcgca gagcagagat tcgattgcgc cgagcattgc
     3961 cattgagaat gccgcaggca gcagtgtgcg aatagaatcc caggttagcg tgaattctga
     4021 attaggcaga tcccacggca gcaccagttg cggcagcagt tgcggaatac cgttaccctg
     4081 agaaccatcg gccaggacgt agtggaattg cgaaccgatg gtagcaacat gtccgccgag
     4141 caggttaaca atccccatca ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa
     4201 acgaatgccc agacgcggcc agaaaacaag aatacctagc gtcacaatgc caatggcagc
     4261 atcacccaca ttaatggtcg gcagcgccat aaataatgcg ccgacttttt gtagataatg
     4321 ttccgggaca tgggccattt gcagaccgag aaaatcttta atctgcatgg taccgatggt
     4381 gatcccgata cccgaggtga aacctaaggt gacggaaacc ggaatatact caatcaggcg
     4441 accaaagcgt gccagaccca tcagaatcaa aaaaatcccc gacagcaagg tcgcaaccag
     4501 cagtcctgcc agtccaaact gttgcgacac gggatagaga attaccacaa atgccgcagt
     4561 cggaccggaa acgctaaagc gtgacccacc cgtcagagca atgacaatcc ccgcaacagc
     4621 tgcggtatat aaaccgtact ggggtgccac accactacca atagccaacg ccatcgccag
     4681 cgggatagca ataatcccga cggttatccc ggcaatcagg tcacgggtaa accgtgcggc
     4741 agtatatttt tctttccagc aagcgtcgat cagagcgcgg aaaggcatca catgtgagga
     4801 aaatattttg ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca
     4861 ggtgaaggag gcataggtca tacaaatgga tattacagac aaaaaaaccc gccgcagcgg
     4921 gtctttgagc cgggttcgat tagtgttcga acatggcaga gatcgattct tcgttgctga
     4981 tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg ttcggcagtg
     5041 atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca attacagagt
     5101 tacgcaggtt attcgccgcg ttgccagaga agatcgggtg agtcgcgtac gcaaatacac
     5161 gtttagcacc acgttctttc agagcttcag cagctttaca cagcgtaccg ccagtgtcga
     5221 tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata tgcatcacct
     5281 gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc catatcggta tcgttcagca
     5341 gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc acaattgggt
     5401 tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat acgttatcaa
     5461 ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc agcacacggt
     5521 caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt acacgagcgg
     5581 aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg gtgatacgac
     5641 ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg ttgtcgttag
     5701 taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt tcgttgattt
     5761 gtacactgac ttcgccgtcg ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca
     5821 ggcggttggc aatacgttgt gctagttccg gggtggcgtt accagcaaaa agcttcatat
     5881 caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt
     5941 gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca acgtctggaa
     6001 caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct ctgtgcaatg
     6061 gggaaagatt aacgcctttc gccacaaagc cattgagcca ttccggggct tgctctagca
     6121 cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct gtcccagtca
     6181 ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt
     6241 ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt tctattgacc
     6301 tttttggcgt attgcgcggg agttcaggat ctttaaaaat caccggagtc ggaatactta
     6361 caccagggtg cgccaccaga taccacttct ctggcggatc caccggcgtt agtatttcac
     6421 caacgccttc ggcaaacgag gcatgccccc gaacaaagac aggaacatct gcgcccagcg
     6481 tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga tgatttaatg
     6541 ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc atcggcaaac
     6601 gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag
     6661 ttttcatcaa caatcgcgct gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa
     6721 cgggcgttaa cagacgaata tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt
     6781 aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa
     6841 tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt atttcactat
     6901 ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca tattggctgg
     6961 catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt ttttgccatt
     7021 ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc
     7081 acccggtaaa cctaaaatcc actggcgcaa gctgttgagc ggaattggca ttccggtcaa
     7141 tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat tgtcgactaa
     7201 ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg ggttagtgag
     7261 cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt acactttttg
     7321 ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat tgcgcacgtc
     7381 ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt
     7441 aacggaacag gcagtgagca caagagcagc cagcggtagc aggcggataa gacgaaaatc
     7501 gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac ccttaatgct
     7561 agcgttaccg tccgctatcg tctatgttca agttgtctta attgccagaa tctaacggct
     7621 ttcggcaatt actccaaaag ggggcgctct cttttattga tcttacgtat cctgtatgat
     7681 gcaagcagac taaccctatc aacgttggta ttatttcccg cagacatgac ccttttagca
     7741 ctcggtatca accataaaac ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg
     7801 gataagctcg atcaggcgct tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg
     7861 gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg ttgaagagca ggataacctg
     7921 caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga agatctgcgt
     7981 aaaagcctct actggcatca ggataacgac gcggttagcc atttaatgcg tgttgccagc
     8041 ggcctagatt cactggttct tggggagccg cagatcctcg gtcaggttaa aaaagcgttt
     8101 gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct
     8161 ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg gtgccagtgc tgtgtctgtc
     8221 gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac ggtcacagtg
     8281 ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa
     8341 gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaattct ggcagatgaa
     8401 gttggcgcgg aagtgattgc cctgagtgag atcgacgaac gtctgcgcga agccgatatc
     8461 atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt ggagcgcgca
     8521 ttaaaaagcc gtcgcaacca accaatgctg ttggtggata ttgccgttcc gcgcgatgtt
     8581 gagccggaag ttggcaaact ggcgaatgct tatctttata gtgtggacga tctgcaaagc
     8641 atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc
     8701 gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt
     8761 cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt taaccgccaa agcgttagcg
     8821 gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg gaaactgact
     8881 aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga cggggataac
     8941 gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat cattttcttt
     9001 ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg gaagccctgc
     9061 atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc gccgaccagg
     9121 aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta
     9181 ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc gatgatcctg
     9241 aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg
     9301 aacagcaatt acaggttctg ttactgccaa aagatcctga tgacgaacgt aacgccttcc
     9361 tcgaagtccg agccggaacc ggcggcgacg aagcggcgct gttcgcaggc gatctgttcc
     9421 gtatgtacag ccgttacgcc gaagcccgcc gctggcgggt agaaatcatg agcgccagcg
     9481 agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg
     9541 gtcgtctgaa atttgaatct ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc
     9601 agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg cctgacgcag
     9661 aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg tcaggggcgg
     9721 gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg ccgaccggga
     9781 ttgttgttga atgtcaggat gaacgttcac aacataaaaa caaagcgaag gcgctttcag
     9841 tgctcggtgc tcgcatccac gctgctgaaa tggcaaaacg ccaacaggcc gaagcgtcta
     9901 cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact tacaatttcc
     9961 cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgcctg gatgaagtga
    10021 tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag gccgaccaac
    10081 tggcggcgtt gtccgagcag gaataatgga atatcaacac tggttacgtg aagcaataag
    10141 ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg agcatgttac
    10201 cggcagaggg cgtactttta ttctcgcctt tggtgaaacg cagctgactg acgaacaatg
    10261 tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg ctcatttaac
    10321 cggggtgcga gaattctggt cgttgccgtt atttgtttcg ccagcgacct taattccgcg
    10381 cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac aaccttgccg
    10441 tattcttgat ctcgggacgg gtaccggggc gattgcgctt gcgctggcta gcgagcgccc
    10501 ggactgcgaa attatcgctg tagatcgtat gcctgatgct gtctccctgg cacaacgtaa
    10561 tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt ttagcgcgct
    10621 agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg agcaggaccc
    10681 acatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg cggcagacag
    10741 tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg tatccggcgg
    10801 ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacaag catttatcct
    10861 cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc gcgtaacgct
    10921 cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct tattagtatc
    10981 gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa gcatccacag
    11041 gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacggtgtt attgttaagc
    11101 ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc acagtggctg
    11161 actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc actcgattat
    11221 cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg
    11281 tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt catgagatcg
    11341 ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa
    11401 gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg tctcgttagt
    11461 ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagctgga aaaactgatc
    11521 gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gcgtttatcg tctttccgat
    11581 gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc attaggtgcg
    11641 gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat tttccctacg
    11701 cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa tccttttaac
    11761 ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat cagcccgtcg
    11821 gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg
    11881 ctggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc gttacgcacc
    11941 agcgaagctt tattacaatt caaccctgaa gatccctatg aaattcgcga tcgcgggttg
    12001 atttatgtgc aactggattg cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa
    12061 cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa catcgcgcat
    12121 aaacatattg tgctgcatta attaatcgac attttactca agattaaggc gatcctatga
    12181 aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt
    12241 ttggcggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc gagcactacg
    12301 taactgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt gacaaagcca
    12361 accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg aaaatcttcc
    12421 aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg
    12481 cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga
    12541 ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt
    12601 ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa
    12661 aagtgattct ttgcgatcgt ggtgctaact tcggctatga caacctggtt gtcgatatgc
    12721 tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc
    12781 acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg
    12841 ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt gaagcgcatc
    12901 cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct aaactggaac
    12961 cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa gaactggata
    13021 ccagcaagta atcttttttg cttgaaaaat aaagtcttag cgttctgcgt taagactttt
    13081 ttcatgggtg ccagatacaa aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc
    13141 gcatattgcc atcacggcag cctgacgccc gttttcacct tacttccggt tacgccacca
    13201 gc
//
LOCUS       DAZFDP010000131        13153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFDP010000131 DAZFDP010000000
VERSION     DAZFDP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..200)
                     /locus_tag="ACHJ1G_003845"
     CDS             complement(<1..200)
                     /locus_tag="ACHJ1G_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFO4765620.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTP"
     gene            519..1100
                     /gene="mdaB"
                     /locus_tag="ACHJ1G_003846"
     CDS             519..1100
                     /gene="mdaB"
                     /locus_tag="ACHJ1G_003846"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO4765621.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1131..1445
                     /locus_tag="ACHJ1G_003847"
     CDS             1131..1445
                     /locus_tag="ACHJ1G_003847"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4765622.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1493..3385)
                     /gene="parE"
                     /locus_tag="ACHJ1G_003848"
     CDS             complement(1493..3385)
                     /gene="parE"
                     /locus_tag="ACHJ1G_003848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4765623.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3414..3995)
                     /gene="yqiA"
                     /locus_tag="ACHJ1G_003849"
     CDS             complement(3414..3995)
                     /gene="yqiA"
                     /locus_tag="ACHJ1G_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4765624.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3995..4822)
                     /gene="cpdA"
                     /locus_tag="ACHJ1G_003850"
     CDS             complement(3995..4822)
                     /gene="cpdA"
                     /locus_tag="ACHJ1G_003850"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4765625.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4847..5269)
                     /locus_tag="ACHJ1G_003851"
     CDS             complement(4847..5269)
                     /locus_tag="ACHJ1G_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4765626.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5270..5899)
                     /gene="nudF"
                     /locus_tag="ACHJ1G_003852"
     CDS             complement(5270..5899)
                     /gene="nudF"
                     /locus_tag="ACHJ1G_003852"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO4765627.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6104..7585
                     /gene="tolC"
                     /locus_tag="ACHJ1G_003853"
     CDS             6104..7585
                     /gene="tolC"
                     /locus_tag="ACHJ1G_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4765628.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7733..8404
                     /locus_tag="ACHJ1G_003854"
     CDS             7733..8404
                     /locus_tag="ACHJ1G_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4765629.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8410..9570
                     /locus_tag="ACHJ1G_003855"
     CDS             8410..9570
                     /locus_tag="ACHJ1G_003855"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4765630.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9608..10396)
                     /gene="ygiD"
                     /locus_tag="ACHJ1G_003856"
     CDS             complement(9608..10396)
                     /gene="ygiD"
                     /locus_tag="ACHJ1G_003856"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4765631.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10539..11312
                     /gene="zupT"
                     /locus_tag="ACHJ1G_003857"
     CDS             10539..11312
                     /gene="zupT"
                     /locus_tag="ACHJ1G_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4765632.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11370..11540)
                     /gene="yqiD"
                     /locus_tag="ACHJ1G_003858"
     CDS             complement(11370..11540)
                     /gene="yqiD"
                     /locus_tag="ACHJ1G_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4765633.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11801..12454)
                     /gene="ribB"
                     /locus_tag="ACHJ1G_003859"
     CDS             complement(11801..12454)
                     /gene="ribB"
                     /locus_tag="ACHJ1G_003859"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4765634.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12558..12706)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12828..13118
                     /gene="ubiK"
                     /locus_tag="ACHJ1G_003860"
     CDS             12828..13118
                     /gene="ubiK"
                     /locus_tag="ACHJ1G_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4765635.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3352 a         3497 c         3199 g         3105 t
ORIGIN      
        1 ggcgtaagaa gaaaaaatgc tgcaatcatt gcttgcaata tacgatcttc gcctgaaacg
       61 gaaggtatac tgcaaatatt gtctcccgac tcaagcatcc agtcatactt attgaaagca
      121 aaaaaagcat ctattaacca ataagtaagc attatgtata acagagcttc aaatattgtt
      181 tgaattttat gctttaacat atatcagtac gctcataaaa atccatttat aattatcaac
      241 atgacacgtt gatattttca tcttttactt attcaaagcc atattttcaa tcccaaaaaa
      301 aaccgaacca aacgtgtaaa ttattccggt gcgagtaaga attatgagga atggctatca
      361 gtattgtcat tttcagaaaa tatttatcct gcatcggtga gtcagagcaa gatcagactt
      421 ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg ctctgtgacg
      481 tactatagtc ggcaaacgtc tcaccttgag gttaaaaaat gagcaacatc ctgattatca
      541 acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg accgaagtcg
      601 cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc gacagcgact
      661 acgatgtcaa agcggaagta caaaactttc tctgggctga tgtggtgatc tggcagatgc
      721 caggctggtg gatgggcgcg ccgtggacag tgaaaaaata tattgatgat gtattcaccg
      781 aaggtcatgg aacgctgtat gccagcgatg gccgtacccg caaagatccg tcaaaaaaat
      841 acggttccgg cggcctggta cagggcaaaa aatatatgct ttctctgacc tggaacgcac
      901 caatggaagc cttcaccgaa aaagatcaat tcttccacgg cgttggcgtc gacggtgtgt
      961 atctgccgtt ccataaagca aaccaattcc ttggcatgga accgctgccg acatttatcg
     1021 ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat cgcaagcatc
     1081 ttgtggaaat ttttggttaa ctagagctca ggctttagaa ggagttaacc atgcttaccg
     1141 taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcggta ttggatcagt
     1201 ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat gcgccaatgg
     1261 tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc gtgatgattg
     1321 agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac atgaaggcgt
     1381 acagcgaagc cgtaaaaggc gacgtgctgg agatgaatat ccgcatcctg caacctggga
     1441 tttaaacctg ccttgtttgc ccggccatcc tgaccgggca atgttctttc ctttaaacct
     1501 caatctccgc catgtcgcct ttctcttgca accagttgcg gcgatcttcc gagcgtttct
     1561 tcgccagcag catatccatc atcgcgtcag tacgctgatc gtcttcatca tcgatagtca
     1621 actgcaccag acggcgagtg ttcggatcaa gcgtggtttc acgcaattgc atcgggttca
     1681 tttcccccag acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt tttaattgct
     1741 caagtacgcc ctctttctct tcttccgtca gcgcgtaata aacctctttc ccgagatcaa
     1801 tacggtagag cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac gcgcggaagt
     1861 gttttacgaa caaagcgcag agcagcgtgg caatgtgcag accatcagag tccgcatccg
     1921 cgaggataca gattttgcca taacgaagct ggctcagatc gtcgctgtca ggatcgatac
     1981 cgatcgctac cgaaatatca tgcacttcct gcgaggccag cacttcgtcg gaagaaactt
     2041 cccaggtgtt aaggatctta cctttcagtg gcatgatcgc ctgatattcg cgatcgcgcg
     2101 cctgcttggc agatccgcct gcggagtcac cttccacaag gaacagttcg gtacggttaa
     2161 gatcctgcgc ggtacagtca gccagtttgc caggcagtgc tgggccgctg gtcagctttt
     2221 tgcgcaccac ttttttagcc gcacgcatac ggcgctgggc gctggaaatc gccatctccg
     2281 ccagcagctc cgccgcctga acgttctggt tcagccacag gataaaggca tctttcacca
     2341 cgcctgaaac gaatgccgcg cattgacgcg aagagagacg ctctttggtc tgcccggcaa
     2401 actgcggatc ctgcattttc accgacagaa cataggcgca gcgatcccag atatcttccg
     2461 ccgacagctt cacgccgcgc ggcagaatgt tgcggtattc acagaactca cgcatcgcgt
     2521 ccaacaggcc ctgacgcaga ccattaacat gggtaccgcc ctgcatcgtt gggataaggt
     2581 tgacgtagct ttcggtcagc agttcaccgc cttccggcag ccacagtagc gcccagtcca
     2641 cagcttcagt atcgccagcg aaattaccga taaacggttt ttctggcagc gtcggtaaac
     2701 cgtttaccgc ttccgccagg taatcattca gaccgtcctg atagcaccag cgctgttcgg
     2761 tgttgttgat ctcatcttta aaggtaatct caacgccggg gcacagtact gctttggcct
     2821 tcagcacatg cgtcaggcgt gaaacagaaa aacgcgggct gtcaaagaag gtttcatccg
     2881 gccagaagtg cacgctggta ccggtattgc gtttaccgca agtgccgaca acctgtaaat
     2941 cctgcacctt ttcgccattt tcaaaggcga tgttataaac ctgaccatcg cggcgcacgt
     3001 taacttctac gcgcttcgac agggcgttaa ccaccgaaat ccccacgcca tgcaggccgc
     3061 cagagaactg gtaatttttg ttagagaatt taccgcctgc atgcagacgg caaagaatca
     3121 gttcaaccgc cggtacaccc tcttccgggt gaatatccac cggcatcccg cgcccatcgt
     3181 caataacttc taacgactgg tcagcatgta agattacatc cacgcgtttt gcgtgacccg
     3241 ccagtgcttc atcgacactg ttatcaataa cttcttgccc caaatggtta gggcgagtgg
     3301 tatcggtata catccccgga cggcggcgaa ccggctcaag cccggtgagt acctcaatgg
     3361 catcagcgtt ataagtttgc gtcatggttt aagttagcaa ttcgagttga tcgtcagaga
     3421 tggtgcagac caagaaaatc gacgatcggg ttgaaataat cttcgaagcc cgtgaatgcg
     3481 tggttgccgc cttctatgac agtctggcgg caggaagcgt agtacgccac cgcctggcgg
     3541 taatccagca cttcatctcc cgtctgttgc agcagccaga tcaaatccgg cgcttccagc
     3601 gggtcaatct gcatgacttt gagatcgtaa atatggcgtg actctagcac atattgctgc
     3661 ccggtgtagg ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa cgggcgcacc
     3721 gccgggttta ccaccactgc gggcagcata aaacattgtg acaaccaggt ggcgtaatat
     3781 ccccccagtg acgaaccgac aatacccagc gaatcgccgc catgttccag gacaatggat
     3841 tccagcagct ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat catctcaacg
     3901 tcagggtgat gttccgccag ccagtttttt aacaagctcg cttttgcaga gcgcggcgag
     3961 ctgttgaaac cgtgtaaata aagaagcgta gacatcagta gccttctgaa gcggtatcag
     4021 gttggaaacg tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca tcagcatgta
     4081 actcgagagt acgccagccg ggcgcgatgg tgtccagcgt aaagttggaa cagtgcggct
     4141 taaactgcac acaggtcgac ggcgttgcca gcaggcggcg accgttccag tcgagatcca
     4201 gctcctgatg aatatgaccg cacagcaagt atttgacgtg cggaaacttc gccagcacgg
     4261 tatccagttc gcccgcgtta cgcaggctgt gttgatcaag ccaactacaa cccgcaggta
     4321 gcggatgatg atgcagcagc agcaacgtat ggcgttctgg cgcatcggcc agtttacgtt
     4381 ccagccactc aagctgaaac tcgctcagct caccgtgcgg cacgccaaac acctggctat
     4441 ccagcaacag gatttgccat tgctcaccaa taaacacccg cttcgccggg gagatacccg
     4501 catcctgtaa cgcgctgtac atcgcgggct ggaaatcgtg gttgccaggc agccagacgc
     4561 agggcgcacg aaaacttgcg atgccttcag cgaaatgccg ataggccgca gaggattgat
     4621 cctgcgctaa atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt ggacgaatcg
     4681 cctccagcac cgcctggtag ctttcccagg tatttacccc taacagggct tcgtgctttt
     4741 gtgcaaacag gtgagtatcg gtaatttgta aaatcctgac tctggcctca ccagccagag
     4801 gaagggttaa caggctttcc aaatggtgtc cttaggtttc acgacgctag taaaccggaa
     4861 tcgccatcgc tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac tggttaattt
     4921 gatgcttttc gtcgcgttga tgcaactttt tattaggata atcataccgc gctttgaagc
     4981 gaaaaatctg ctggcttgaa cacacttcag ccaccatcgc gtcatgatac agacgcaccg
     5041 tcattgacgg aaggctccag taactgatcg cgggcgcagt ctgttctatt gtcaccaggg
     5101 tagtgtatcg ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc acctgatagc
     5161 ttacagtttc gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt gaaaagttca
     5221 tctcgcacag gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt tatgcccact
     5281 cattttttaa cgcttgatga tgcagctgca gccattgcaa agcgatgacc gacgctgcgt
     5341 tgtcgatttt cccctcttct acccactggt atgcctgttc ccggcttacc acatgaacgc
     5401 gaatatcttc gttttcatca gccagaccat gaataccgct tgcggtcgtg gcgtccactt
     5461 cgcccaccat aattgacgaa cgctcactgg tgccccccgg gcttgccagg aaacttaaca
     5521 ccggtttggt ccgtttgact atcagtcccg cctcttcaat cgcttcgcga cgggcaacat
     5581 cttccacact ttcaccctct tctatcatcc cggcaaccat ctccagtagc cagggggttt
     5641 cgctggtgtc gtacgcggca atccgaatct gctcaatcag cacaacttca tcacgcactg
     5701 ggtcaaaggg tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc cgtacctcat
     5761 gactcatttg cccattgaat agacgatgac gaaatctata aagatctaat gaaaaaaagc
     5821 cgcgataaag tgtttctcgt gcaataattt ctacatcgtt tttgccaaat gtaacgggca
     5881 ggttgtctgg cttaagcatt gttaatgtcc tggcactaat agtgaattaa atgtgaattt
     5941 cagcgacgtt tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca ttaacgccct
     6001 atggcacgta acgccaacct tttgcggtag cggattctgc tagaatccgc aataatttta
     6061 cagtttgatc gcgctaaata ctgcttcacc acaaggaatg caaatgaaga aattgctccc
     6121 cattcttatc ggcctgagcc tttctgggtt cagttcgttg agccaggccg agaacctgat
     6181 gcaagtttat cagcaagcac gccttagtaa cccggaattg cgtaagtctg ccgccgatcg
     6241 tgatgctgcc tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac agctaggttt
     6301 aggtgcagat tacacctata gcaacggcta ccgcgacgcg aacggcatca actctaacgc
     6361 gaccagtgcg tccctgcagt taactcaatc catttttgat atgtcgaaat ggcgtgcgtt
     6421 aacgctgcag gaaaaagcag cagggattca ggacgtcaca tatcagaccg atcagcaaac
     6481 cttgatcctc aacaccgcga ccgcttattt caacgtgttg aatgctattg acgttctttc
     6541 ctatacacag gcacaaaaag aagcgatcta ccgtcaatta gatcaaacca cccaacgttt
     6601 taacgtgggc ctggtagcga taaccgacgt gcagaacgcc cgcgcgcagt acgataccgt
     6661 gctggcgaac gaagtgaccg cacgcaataa ccttgataac gcggtagagc agctgcgcca
     6721 gatcaccggt aactactatc cagaactggc agcgctgaat gtcgaaaact ttaaaaccga
     6781 caaaccacag ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc tgtcgctgtt
     6841 acaggcacgc ttgagccagg acctggcgcg cgagcaaatt cgccaggcgc aggatggtca
     6901 cttaccgacg ctggatttaa cggcttctac cgggatttct gacacctctt atagcggttc
     6961 gaaaacccgt ggtgccgctg gtacccagta tgacgacagc aatatgggcc agaacaaagt
     7021 gggcctgagc ttctcgctgc cgatttatca gggcggaatg gttaactcgc aggtgaaaca
     7081 ggcacagtac aactttgtcg gtgccagcga gcaactggaa agcgcgcatc gtagcgtcgt
     7141 gcaaaccgta cgttcctcct tcaacaacat taatgcatct atcagtagca ttaacgccta
     7201 caaacaagcc gtagtttccg ctcaaagctc attagacgcg atggaagcgg gctactcggt
     7261 cggtacgcgt accattgttg atgtgttgga tgcaaccacc acgctgtaca acgctaagca
     7321 agagctggca aatgcgcgtt ataactacct gattaatcag ctgaatatta agtcagccct
     7381 gggtacgttg aacgagcagg atctgctggc actgaacaat gcgctgagca aaccggtttc
     7441 cactaatccg gaaaacgttg ccccgcaaac gccggaacag aatgctattg ctgatggtta
     7501 tgcgcctgat agcccggcac ccgtcgttca gcaaacatcc gcacgcacta ccaccagtaa
     7561 cggtcataac cctttccgta actgatgacg acgacggggc ttcggccccg tctgaacgta
     7621 aggcaacgta aagatacggg ttatctgccg cattcttccc ccttctcgct tcaatttcga
     7681 ccagccatcc tctattctga tgggtattta ccactggtcc cggaagacaa aaatgaaacg
     7741 gacaaaatcc atacgccacg catcgttccg caaaaactgg agcgcgcgcc atctgacacc
     7801 agtcgctctc gcggttgcca ctgtttttat gctggctggc tgtgaaaaga gtgatgaaac
     7861 agtgtctctc tatcaaaatg ctgacgactg ttcagctgca aacccaggca aaagcgccga
     7921 atgtaccacc gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc cgaaatacgc
     7981 cacccgtgaa gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg caccagccca
     8041 ggctggcatg gcaccagaaa accaggcgca ggcccagcaa tccagcggga gtttctggat
     8101 gccgctgatg gccggttaca tgatggggcg tctgatgggc ggcggcgcgg gatttgcaca
     8161 gcagccgctg ttctcctcga aaaacccagc cagcccggct tacggtaaat ataccgacgc
     8221 gacgggtaaa aactatggcg cagcccagcc aggccgcacc atgaccgtac cgaagacagc
     8281 catggcacca aaaccggcga ccaccactac cgttacccgt ggcggttttg gtgaatctgt
     8341 tgccaaacaa agcactatgc agcgtagcgc aactggcacc tcttctcgtt caatgggtgg
     8401 ctgataccga tggaaagagt cagtattacc gagcgcccgg actggcgtga gaaagcccac
     8461 gaatacggtt tcaattttca caccatgtac ggcgagccgt actggtgtga agatgcttac
     8521 tacaagttga ccctcgccca ggttgaaaag ctggaagaag tcaccgccga actgcaccag
     8581 atgtgcctga aagtggtgga aaaagtgatc gccagcgatg agctgatgac caaattccgc
     8641 attccaaaac acacctggag ttttgtgcgc cagtcatggc tgacgcacca gccatcgctt
     8701 tattcgcgtc ttgatctggc gtgggatggc actggcgaac ctaaacttct ggaaaataac
     8761 gccgatacgc caacgtcact atacgaggcg gcgttctttc agtggatctg gctggaagat
     8821 cagcttaacg ccggtaactt gccggagggc agcgaccagt ttaacagtct gcaagaaaaa
     8881 ctgatcgatc gcttcgttga gctgcgtgaa cagtatggct tccagttgct gcatctcacc
     8941 tgctgtcgcg acacggtgga agatcgcgga accattcagt atttgcagga ctgcgcaacg
     9001 gaagctgaaa ttgctactga gttcctctac atcgatgata tcgggttagg tgaaaaaggt
     9061 cagttcacgg atttacagga tcaggtaatt tccaacctgt tcaagctgta tccgtgggaa
     9121 tttatgttgc gtgagatgtt ttccaccaag ctggaggatg caggcgtacg ctggctggaa
     9181 ccggcgtgga agagcattat ctccaacaag gcacttctac cgctactgtg ggagatgttc
     9241 ccgaatcacc cgaacctgct gcccgcttat tttgcggaag atgatcatcc gcaaatggaa
     9301 aaatatgtgg ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat cattgaaaac
     9361 ggcaaaacca ttgaagcagc ggaaggtccg tatggcgaag aagggatgat tgttcagcaa
     9421 ttccacccgt taccgaaatt cggcgacagc tatatgctga ttggtagctg gctggtgaac
     9481 gatcaacccg ccggaattgg cattcgtgaa gaccgtgcat tgatcaccca ggatatgtct
     9541 cggttttatc cacatatttt tgttgagtaa tcaatgatgc cggatggcat tcgccatccg
     9601 gtaattgtta acctatctgc accgacagca tactcaggct gcccatttct ataccatcga
     9661 ccggaatggt aattggctcc tgcccatccc acgcacctaa gacatacaac aacggcagat
     9721 agtgctccgg cgttgggttc gataacgcgc caccttcatg gtcgaggtaa ttcaccagag
     9781 gatgctgctc taccgcccct tgccacgtca gattcgcttt cacatactca ttaaacgaca
     9841 tcgcccaagg atatggtgaa ctatcaccgt gccacttcac tgtacgcagg ttatgcacca
     9901 cgttgccact ggcgaccaac attattcctt catctcgcag cgctgccagt ttgcgcccca
     9961 tttcgaaatg ccaggcggca ggtttgctac tgtcgatact caactgcacc atcgggatat
    10021 cggcgtcagg atacatctta atcagcacgc cccaggaacc gtggtcaaag ccccaggctt
    10081 ctttatccag cgtcaccggg accggtgcta acagctcaac cagatgctgt gccagcacag
    10141 gcgaacccgg agcaggataa tgcgtatcgt acagcgcctg cgggaagcca ccaaagtcat
    10201 gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac cagtgagccg
    10261 aaaccaccac aatcgcttgc gggcgtggca atgtcatccc caacttctgc cagctgcggg
    10321 tatacaaatt atcttccagc acgttcatcg gactaccgtg acctaaaaac aatgctggca
    10381 tacgtgttga agacatgatg atatccttaa ctaaaggtgt cattttgata tcctcacaat
    10441 acgcttgttc ggcggagtaa gaacccggat aacaatgatg atgatcatca gttattttga
    10501 cgatctgcct gaaggtgaag atttataagg agttgtcgat gtcagtacct ctcattctga
    10561 ccatactggc gggggcagcc acgtttattg gcgcgtttct cggcgttctc gggcaaaaac
    10621 cctcgaaccg cttactggcg ttttcgctag gatttgcggc ggggatcatg ttgctcatct
    10681 cattaatgga aatgcttcct gccgcactag cagctgaagg aatgtcgcct gtgttgggtt
    10741 atggaatgtt tatcttcggt ctgcttggct attttggcct ggaccgcatg ttgccacatg
    10801 cgcatccgca ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg atcaagcgca
    10861 cagccattct gctcactctc ggcatcagtc tgcataactt cccggaaggg attgccacct
    10921 ttgtcacggc gagcagcaac ctggagctgg gatttggcat cgcactggcc gtcgcgttgc
    10981 acaatattcc tgaaggtctg gcggttgcag gcccggttta tgcagcaacg ggttctaaac
    11041 gcacggcaat tctgtgggcg gggatttctg gattggcaga aattcttggt ggtgtgctgg
    11101 cgtggttaat cctcggtagc atgatttccc ccgtggtcat ggcggcaatc atggcggcgg
    11161 ttgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc aaagagattg
    11221 accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg ggattcagtt
    11281 tagtgctgct acaaacggcg ggaattggtt aaaagcaaca tcagaagttg gccagcgcat
    11341 ggacaacttc tgctagcttc actaaagcct tattctacct tttcggctgt ctcttctctc
    11401 gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg gcgaaacgcc
    11461 cgacaataac gtttcaaatg caggaaataa cccaccacaa caagagcaat caatacaatc
    11521 cagtaccagg cgataaacat ccttttaccc ttaatattta acaatactaa acatttagcg
    11581 cataaatttc acatatcctt tttcggatat attcatatgg tcgcataata acggaacaat
    11641 tatatgattt tgatcataat gaaaaagaat gcctgatatt aatgaaattt tctcatagaa
    11701 agagaagcgg aattaacatc ttgcatagca gggcaaaata aaaaccgaag ccagctacaa
    11761 cttcggtttc ttgattaagg cagtaattaa gcagcggttt tcagctggct ttacgctcat
    11821 gtgcctgacg gtatgccacc aggtcttcaa tagtcacgag cgccatattg tgtttattgg
    11881 caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc aactcacaca
    11941 gcacaccagc cggtttaaag cctgccagcg tcatcaaatc aatagtcgct tcagtatgac
    12001 cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg cccgggcgat
    12061 tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta attcgatcag
    12121 cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta aaaccggtgc
    12181 cataggcgct ggtgttattt tctaccatca ttggcagatc aagttgttta cggcgatctt
    12241 cagtgatgca caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc atctgctcaa
    12301 cggtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct tcatcatcaa
    12361 gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca cgttcgaaag
    12421 gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac taaaattatg
    12481 gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa cgtgagcggg
    12541 tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg gccccggaat
    12601 tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct ttcaactgag
    12661 ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat tcactataac
    12721 gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg acttaccctt
    12781 atcgcactac aatggcactc aacacctatc agtacaggga aactacaatg attgacccga
    12841 aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga atcagggagt
    12901 tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agctcagctg acgcgcctcg
    12961 accttgtaag ccgtgaagag ttcgacgtcc aaacgcaagt cctgttacgg actcgtgaga
    13021 aactggcgct gcttgaacaa agaatgagcg aactggaaaa tcgcagtact gagattaaaa
    13081 agcagccaga tccagagact ctccccccaa cgctgtaaac atttgtcttt aatgcactgt
    13141 aaattatgaa tta
//
LOCUS       DAZFDP010000132        13085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFDP010000132 DAZFDP010000000
VERSION     DAZFDP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..466
                     /locus_tag="ACHJ1G_003861"
     CDS             <1..466
                     /locus_tag="ACHJ1G_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4765636.1"
                     /translation="SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLE
                     MVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILR
                     GEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(620..2269)
                     /gene="yjcE"
                     /locus_tag="ACHJ1G_003862"
     CDS             complement(620..2269)
                     /gene="yjcE"
                     /locus_tag="ACHJ1G_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4765637.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2421..3770)
                     /gene="ghxP"
                     /locus_tag="ACHJ1G_003863"
                     /gene_synonym="yjcD"
     CDS             complement(2421..3770)
                     /gene="ghxP"
                     /locus_tag="ACHJ1G_003863"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4765638.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4316..4780)
                     /gene="soxR"
                     /locus_tag="ACHJ1G_003864"
     CDS             complement(4316..4780)
                     /gene="soxR"
                     /locus_tag="ACHJ1G_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4765639.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4866..5189
                     /gene="soxS"
                     /locus_tag="ACHJ1G_003865"
     CDS             4866..5189
                     /gene="soxS"
                     /locus_tag="ACHJ1G_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4765640.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5192..6778)
                     /gene="pdeC"
                     /locus_tag="ACHJ1G_003866"
     CDS             complement(5192..6778)
                     /gene="pdeC"
                     /locus_tag="ACHJ1G_003866"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4765641.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7208..7489
                     /gene="yjcB"
                     /locus_tag="ACHJ1G_003867"
     CDS             7208..7489
                     /gene="yjcB"
                     /locus_tag="ACHJ1G_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4765642.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7588..8124)
                     /gene="ssb1"
                     /locus_tag="ACHJ1G_003868"
     CDS             complement(7588..8124)
                     /gene="ssb1"
                     /locus_tag="ACHJ1G_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4765643.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8378..11200
                     /gene="uvrA"
                     /locus_tag="ACHJ1G_003869"
     CDS             8378..11200
                     /gene="uvrA"
                     /locus_tag="ACHJ1G_003869"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4765644.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11235..11591)
                     /gene="yjbR"
                     /locus_tag="ACHJ1G_003870"
     CDS             complement(11235..11591)
                     /gene="yjbR"
                     /locus_tag="ACHJ1G_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4765645.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11595..12011)
                     /gene="yjbQ"
                     /locus_tag="ACHJ1G_003871"
     CDS             complement(11595..12011)
                     /gene="yjbQ"
                     /locus_tag="ACHJ1G_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4765646.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12122..12835)
                     /gene="aphA"
                     /locus_tag="ACHJ1G_003872"
     CDS             complement(12122..12835)
                     /gene="aphA"
                     /locus_tag="ACHJ1G_003872"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4765647.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3389 a         3432 c         3204 g         3060 t
ORIGIN      
        1 ctctaaactc gatttaatta actttgaaca tgctgatctg tctcgcgtca atctgaacaa
       61 agcaatcctc cagaatataa acttaattga tagcaaactc ttttgtacgt ggctaacaaa
      121 tacattcctc gaaatggtta tatgtaccgg ctctaatatg gctaatgtta attttaataa
      181 tgccaattta agcaactgcc atttcaactg ttctatttta acaaaagcct gtatgtttaa
      241 tacccgtctc tatcgggtta attttgatga ggctagcgtc cagggaatgg gcatttctat
      301 tctccgtggg gaggaaaata tccccattga tagtgatacc ctggtaacac tacagaaatt
      361 ctttgaagaa gattgtacct ctcatactgg catgtcacaa actgaggata atattaatgc
      421 agtcgctatg aagattactg cagatattat gcaacacgca gattgaggtt aagtcgaaga
      481 acagccaatg aattgacttt acaagagaga taacttattt gttttagaat catgaatgtt
      541 agcccgagcc aacctgttaa aggtgcaaaa gattgcacct ttttcagatt acctcgggcc
      601 tggttacgtc gctttcggct tactgatttt cctcaattag caacgcttca agcaaatcga
      661 gatcgtgcag taatttttgc agcgtttcgt tgctgatctc ccgcgtggcg cgcaggtggt
      721 acagttcagc acgttcagaa cgcaatgccg ccagacggaa gcgacgctca aggttctctt
      781 cctgcacgga actttcaacg tcgttacgtc catcggcgcg acgacgcagg ttaccaatga
      841 cgcgagaact gacctccgta agcagctggt tatcgatatt ctcttcggta tccgccgcca
      901 gacgctcctc cattttctgg atcgccacaa tcgccacttc tgccgttgcc gctcgcgcaa
      961 tacgttcttc tttcaattgt tgcgaatggt ccgcgacttc aatgtgttgt agcagaatag
     1021 gcaacatcac cacgccgaca aacagcgaga agagaatgac gccagccgcc aggaacacca
     1081 gctcatagcg cgccgggaag acgttaccat ccggcaagag cagcgggata gagagcacac
     1141 cggccagagt gatcgcccca cgcaccccgg cgaaagacgc gatcaggatt tctcgtgtgg
     1201 tccacgaacc aaactccatc ggcttcttct tcaggaagcg gttgctgaac tttttcatcg
     1261 tccacagcca gccgaaacgg accagcatca gcgccgcata tatcagaata atatcggtaa
     1321 acagcatcca gatttcgacg ttagggtcga tttctgccgc catcagcgac gtctccagaa
     1381 tacccggcag ctgcagacct aacagcagga acaccatgcc gttaaagaca aattccagca
     1441 tcgcccaggt gctgtttgcg cgcaggcgca ttgccagcgg cgcacggcgc atcacaccgg
     1501 agcgggtgat ggtcatcccg gcggcaaccg cagcgaggat gccggaaacg ccaatatgtt
     1561 cggcaatcag ataagaagcg aatggcagca agaacagcag aacgatctgc gtcgcgggtt
     1621 catcaccgcc ccagcggctg aggaatcgca gcgagcgacc atacagccag ctcaccacaa
     1681 agccggcgag aataccgcca atggctactt tcataaattc gaccgtcgca ccgccaacgg
     1741 tgaagatcat cgtccccatc gccactgcca cggcaaactt caacgacacc aggccggagg
     1801 cgtcattcat caacgcttcg ccctgcaaaa tacccatgat ttttttcggg atgcgccctt
     1861 cgccaacaat cccggagagc gccacagcat cggtcggaga aagcactgcc gccagcgcaa
     1921 aggcggggat cagcggaatg cccggcacca cccagtaaat aaggaagcca atgccgacca
     1981 cggtgaccac caccagcgcc agcgcgaggc caaaaatctc tcgaccatgt tcgagaaatt
     2041 cacgggtcgg cgttttccag ccatcagcga acagcaacgg cgggataaac aagactaaaa
     2101 agagttcagg atcaaactcc acatgcaaac caaacgtcgg ccacgccagt agcgcaccga
     2161 tggcgatttg cataagcgga agcgggatct gaaagggcat gacacgagtg accaccccgg
     2221 acagcgagac cacgagggtc attatcagta tggtgaagaa gatttccatg cgttccctgt
     2281 ttgcgatgtt tttttatgat ctgagaaaga cgttatgcct gaattctcat tttttcagaa
     2341 tatcgcaaaa gctcacacaa tgtgcggaga aaataaaaac gggcagccat tgccacccgt
     2401 tttcagaatc cgatgaaaga ttagatagcc cagccacccg catagaaggt caccagcgcc
     2461 acggcgataa cgaccgtacc gatgttcaac ttgcgccatt cgccggaaac cagacgacca
     2521 atcaccagag tcgcgaagcc gatcatgatg cctgttacga tgttacaggt cagcacgatg
     2581 aataccgccg taaccagacc cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc
     2641 acgttgctca gcatcagcag gccaacgtac atcagcgccg gagccgttgc ataccccgga
     2701 acgaggtaag agagcggaga gaggaacaga atcagcagga acagcacgcc aacggtgata
     2761 gccgtcaaac cggttttacc gcccgccgcc gtacccgccg cagactcgat gtataccgct
     2821 gccggagcag cacccaccag gccagagaaa acgctgctca tggagtcagt ggtcagtgct
     2881 ttcccaccgt cgatgatctg cccatcttta tccagcaggt tcgcctggcc ggcgacggca
     2941 cggatagttc cggtggcatc aaatactgcc gtcatcacca gcgccagaac gcttggcagg
     3001 actacaggat tcagcgcgcc cataatgtcc aggctgccaa tcagtgaatt gccgttttca
     3061 tcgctcaatg aaggcatggc gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa
     3121 ccgacaattg agataccgat aatggtcagc agaatgccac cagggacttt cagtttttcc
     3181 aggccgatga tcaccgccag acctactagt gacataatca ccgggaaggt cgcgaaatca
     3241 cccagcgcaa cgggcagacc atcaagcggg tttttaatca ccagaccgac accattagcg
     3301 gcaatgagca gcaggaacag accgataccg atccccgtgc cgtgcgccac accgtgcggc
     3361 aagttgcgca aaatccagct acggataccc gtggcagaaa ttaccgtaaa cagcaccccc
     3421 atcaggaaca cggcacccag cgcgacaggt acgctaatat gttgccccag caccaggctg
     3481 aatgcggtaa acgccgtcag ggagatggcg caaccaatcg ccaacggcag attagcccac
     3541 agacccatca cgatagaacc gagtccggca accagacagg ttgcaacgaa aactgccgca
     3601 ggcgggaagc ctgctttacc caacatacct ggaacgacga tgaccgagta gaccatcgcc
     3661 agaaacgttg ttaacccggc aaccacttcc tgacggacag tgcttccacg ttgtgaaatt
     3721 ttaaaccagg cgtcgagtga accgccggta cgcgctgatg gcgtagacat aagaaaacat
     3781 cccctgagag tttaattttc gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt
     3841 tatcttcctg ctccaggttt gcgacaattt actgcgtcgc aaaaaagcaa acgtttagct
     3901 ccgcgcatac aaaacgggtg gtaaaaaaag caaacgatta tccggcgatc atagcggctt
     3961 tttcaaccga agtttgctgc ttttttctct tttattgtct tgacgatgaa aaaccaggca
     4021 attgatgcgc aaacgttatc gtcggtgcgc aagattgcag cgttatcgag ccgtataccg
     4081 ttcaggattg cgataaaaaa tcgtctgaga tgacaaaaat attgcggata aaaagtatac
     4141 ttttacactg gcgaagaaat gccggtgaca tacaattaaa gcatcaacac caaccggaac
     4201 ctccaccacg tgctcgaatg gggtgtgttg acgtcggggg aaaccctcct gtgtaccagc
     4261 gggatagaga gaaagacaaa gaccggaaaa caaactaaag cgcccttgtg gcgctttagt
     4321 tttgttcatc ttccagcaag cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt
     4381 tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc aatacatccg tccagttcgt
     4441 cacgcagcgc tactaaggta tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa
     4501 gctgtttcca ctctttcgca cttaacgtat gcccttcggg caacacgcca aacgcttcac
     4561 caatggtcgc cagcggaatg ccaatacgct gagcaatttt gataattgca acatatcgca
     4621 acacatcacg tttatatcgc cgctgattgc cgctgttacg gatactggta atcaaccctt
     4681 tactttcata gaaatgcagc gccgataccg ccacaccgct gcgtttcgcc acttcgccgg
     4741 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
     4801 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
     4861 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg
     4921 accagccgct taacattgat gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc
     4981 aacgaatgtt ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgtcgcc
     5041 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
     5101 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca
     5161 ctcccagcga ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg
     5221 gtaattgctc catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaacatc
     5281 cctggcaata gcgcacgccg cgtttgcgca gccagttaac ctgctcctca gtttcgacgc
     5341 cttcagcgat cgtttttaac cccaggctgt gcgccagctc gatgatgtgt tccgcaatca
     5401 aatgactggt tttgtgggtg gtcagcgttt caacaaacga tttatcgatt ttcaaaatat
     5461 cgacattcaa tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg
     5521 ccacttcgta accagcctgg cggaaagcca gaataatcgg cgtcattttg tcaacatcaa
     5581 gaaatgcatg ttcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg
     5641 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa
     5701 tagaaacata gcgatcggca tgtgttgcca agtacgcgcc cagatcacgg aagacattat
     5761 caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact
     5821 ctgccggatt cattatttgc ccctgctcac caagccaacg taacaacgct tcagcgccga
     5881 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     5941 cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga
     6001 gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat
     6061 aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     6121 tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     6181 tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca
     6241 ccgtatcata cacaccccat tgcaatgtcg gatcatcaga catcacttca ctccagaaga
     6301 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     6361 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg
     6421 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac
     6481 gggcatagat caattcatta atataaagat agccacgaat aatattcaac attcgctttt
     6541 gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg
     6601 cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat
     6661 tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga
     6721 gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg
     6781 ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaaccct gggtaccgac
     6841 gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa ttgatacctt
     6901 tctaatcgtc acctggatga aaataattca ttgatattca aaattaattt cctacaggaa
     6961 tgcagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata atctgtacag
     7021 caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata gttcacaaaa
     7081 ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga tagccagagt
     7141 gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt ttcagaggaa
     7201 ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac ttcttagtgc
     7261 cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg attatgtcgg
     7321 gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg caatggggtt
     7381 gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt tggtgatgat
     7441 agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat acgtagaagc
     7501 ctccataacg gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac
     7561 aatataacct attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg
     7621 cggctcgtta gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct
     7681 gaactgattg ccaccctgcg gctgctgtgg ctgaccccaa ccgccctgcg gctgaccacc
     7741 accgatattg ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt
     7801 gccgccaacg ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca
     7861 tttacgggta cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct
     7921 cgccacttct gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat
     7981 ctcgccggtc gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc
     8041 gccaccattt ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac
     8101 cttgtttacg cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt
     8161 gtaaacgcgc gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg
     8221 ggaaacaaac ctggccagac attgttacac aacactccgg gtaatgcatt ccaatactgt
     8281 atattcattc aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg
     8341 ccaccgggca aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg
     8401 cgcccgcacc cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt
     8461 cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga
     8521 agggcagcgc cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat
     8581 ggaaaagccg gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa
     8641 atcgacgtct cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt
     8701 gcgtttgttg ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc
     8761 ggcgcaaacc gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct
     8821 gatgctgctc gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa
     8881 cctggcaagc cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga
     8941 tccgccgaaa ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa
     9001 ggtgcgtgac gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggagctttc
     9061 cggtggtaca gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc
     9121 cgccaacttc gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt
     9181 ttcgtttaac aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata
     9241 tttcgatcct gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg
     9301 tggctgggat cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta
     9361 taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt
     9421 gtacggttct ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc
     9481 catccgtcgt catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac
     9541 agaatccagt gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag
     9601 ctgcgaaggg acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct
     9661 gcctgctatc tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact
     9721 cgctggtcaa cgggcgaaga ttgcggaaaa aatccttaaa gagatcggcg atcgtttgaa
     9781 attcctcgtt aacgtcggcc tgaattacct gacgctttcc cgctcggcag aaacgctttc
     9841 cggcggtgaa gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt
     9901 tatgtacgtg ctggacgagc catctatcgg cctgcaccag cgcgataacg agcgcctgtt
     9961 gggtacgctt atccatctgc gcgatctcgg taataccgtg attgtggtgg agcacgacga
    10021 agacgcaatt cgcgccgctg accatgtgat cgacattggc ccgggcgcag gtgttcacgg
    10081 cggtgaagtg gtcgcagaag gtccgctgga agcgattatg gcggtgccgg agtcgttgac
    10141 cgggcagtac atgagcggca aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa
    10201 tccggaaaaa gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct
    10261 gacgctgccg gtgggtctgt ttacctgcat caccggggtt tcaggttccg gtaaatcgac
    10321 gctgattaac gacacactgt tcccgattgc ccaacgccag ttgaatgggg cgaccatcgc
    10381 cgaaccagca ccgtatcgcg atattcaggg gctggagcat ttcgataaag tgatcgatat
    10441 cgaccaaagc ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt
    10501 tacgcctgtg cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc
    10561 aggacgtttc agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt
    10621 gatcaaagta gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgtaaagg
    10681 taaacgctat aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt
    10741 gctggatatg accatcgaag aggcgcgtga gttctttgat gccgtacctg cactggcgcg
    10801 taagctgcaa acgttgatgg acgttggcct gacgtacatt cgcctggggc agtccgcaac
    10861 cacactttct ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg
    10921 caccgggcag acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat
    10981 tcagcaactg ctcgacgtac tgcataaact gcgcgatcag ggcaacacca ttgtggtgat
    11041 tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg
    11101 cggcagtggc ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga
    11161 agcttcgcat acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga
    11221 gcggcctttt cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc
    11281 actgcctgct gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg
    11341 tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg
    11401 tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga
    11461 ttttctactt ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc
    11521 cagtcgttat gcacgctctg ttctgcgcct ggttttgcca tgcaatattg tagcaactcc
    11581 gaaatggtca ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg
    11641 atgcgatgtt cccccagcca gatgccttgc caggtgccgg tctgaatacg ccctttatgt
    11701 accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct
    11761 gctccctcat agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacgc
    11821 tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta
    11881 tgttgcagca acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc
    11941 agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt
    12001 tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg
    12061 aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gcgtcacgct ctcccgctct
    12121 gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt
    12181 tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg
    12241 cggtaatatc attatcagaa tcgccataaa aaattcggat atttttatcc tgcagccatt
    12301 gcgattttgt attttgccct agtttatcgc ccgcaaagat caccggattc atgttggtgg
    12361 caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcggactac
    12421 gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag
    12481 cgacctcttt tggaatgctg aattcatccc agccattgtt cattttttcc cagaacacag
    12541 gatttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaagcccg
    12601 gactggaaaa aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg
    12661 cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat gggtgcctgt tcagcaagcc
    12721 tggcaacgtt agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac
    12781 tgtttagagc gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt
    12841 tccctaaata gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg
    12901 accataacgc cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca
    12961 ggatgattat atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta
    13021 ttactcgatg taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag
    13081 cgaaa
//
LOCUS       DAZFDP010000133        13063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFDP010000133 DAZFDP010000000
VERSION     DAZFDP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(135..1559)
                     /gene="patD"
                     /locus_tag="ACHJ1G_003873"
     CDS             complement(135..1559)
                     /gene="patD"
                     /locus_tag="ACHJ1G_003873"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4765648.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1581..2375)
                     /gene="ydcV"
                     /locus_tag="ACHJ1G_003874"
     CDS             complement(1581..2375)
                     /gene="ydcV"
                     /locus_tag="ACHJ1G_003874"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4765649.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2365..3306)
                     /gene="ydcU"
                     /locus_tag="ACHJ1G_003875"
     CDS             complement(2365..3306)
                     /gene="ydcU"
                     /locus_tag="ACHJ1G_003875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4765650.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3307..4320)
                     /gene="ydcT"
                     /locus_tag="ACHJ1G_003876"
     CDS             complement(3307..4320)
                     /gene="ydcT"
                     /locus_tag="ACHJ1G_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4765651.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4338..5483)
                     /gene="ydcS"
                     /locus_tag="ACHJ1G_003877"
     CDS             complement(4338..5483)
                     /gene="ydcS"
                     /locus_tag="ACHJ1G_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4765652.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5728..7133)
                     /gene="ydcR"
                     /locus_tag="ACHJ1G_003878"
                     /pseudo
     CDS             complement(5728..7133)
                     /gene="ydcR"
                     /locus_tag="ACHJ1G_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7212..7628)
                     /gene="hicB"
                     /locus_tag="ACHJ1G_003879"
     CDS             complement(7212..7628)
                     /gene="hicB"
                     /locus_tag="ACHJ1G_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4765653.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7674..7850)
                     /gene="hicA"
                     /locus_tag="ACHJ1G_003880"
     CDS             complement(7674..7850)
                     /gene="hicA"
                     /locus_tag="ACHJ1G_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO4765654.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8072..8302
                     /gene="yncJ"
                     /locus_tag="ACHJ1G_003881"
     CDS             8072..8302
                     /gene="yncJ"
                     /locus_tag="ACHJ1G_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4765655.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8394..10355)
                     /gene="rlhA"
                     /locus_tag="ACHJ1G_003882"
     CDS             complement(8394..10355)
                     /gene="rlhA"
                     /locus_tag="ACHJ1G_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4765656.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGSGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10428..10964)
                     /gene="sutR"
                     /locus_tag="ACHJ1G_003883"
     CDS             complement(10428..10964)
                     /gene="sutR"
                     /locus_tag="ACHJ1G_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4765657.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11017..12231
                     /gene="ydcO"
                     /locus_tag="ACHJ1G_003884"
     CDS             11017..12231
                     /gene="ydcO"
                     /locus_tag="ACHJ1G_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4765658.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12271..>13063)
                     /locus_tag="ACHJ1G_003885"
     CDS             complement(12271..>13063)
                     /locus_tag="ACHJ1G_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4765659.1"
                     /translation="SRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPAHPSASMVGLD
                     AGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSC
                     IANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSI
                     LDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCG
                     YTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3167 a         3509 c         3205 g         3182 t
ORIGIN      
        1 atgaaaaatc gccatcaaac atttagccta atttttacgc tagttattaa tgtaatcatt
       61 ctaatactta accgccatta aactcagaac ctaaaacgat agtggccgtc ctataacagc
      121 cactaacatc taacttaatg tttaaccatg acgtggcgga cgacggtgta atcctccagc
      181 ccataaagtg acatatcctt gccgtaacca gaaagtttct gcccaccgtg cggcatttca
      241 cttaccagca tgaaatgggt attgacccag gtacaaccat attgcagccg tgcgctgacg
      301 cgatgcgccc tgcccacatc tttcgtccat accgaagatg caagtccgta ctggctgtca
      361 ttcgcccagt tcaccacctg ttcttcgttg tcgaagggcg taacactcac tactggacca
      421 aatacctctt tttgcacgat ggcatcgtcc tgtaatgcgc cagccagcag cgtcggcgca
      481 tagtaatagc cattaccctt gcgcttttca ccgccagtga tcactttgat gtgccctgtc
      541 gctttcgcct cttctactgc cttgctgacg cgttcgagat gcgccagcga gcttaaaggt
      601 ccaagctccg tagactcgtc atccggcgca ccagatttta acgttgccac cgcagcaccc
      661 agtttttcca ccagcgtatc gtaaatgcct ttttgcgcgt agatccgaca agccgcagta
      721 caatcctgtc cagcattgta atagccaaat gtacgtacac cttcgaccac tgcttcaata
      781 tccgcatcat caaaaacaat cactggcgct ttgccaccaa gttccatatg agtacgctta
      841 atggacgacg cggtatggct gatgatgtgc tcgccggtgg cgatagagcc cgtcagcgac
      901 accatccgca ctttgggatg accggtcagc ggatcaccca ccgttttgcc tctgccaaac
      961 agtacgttaa tcacgcctgc cgggaagata tctttcgcca gctctgccaa cttcaacgcg
     1021 gtcagcgggg taatttctga tggtttaagc actacgcagt tccctgccgc cagcgccgga
     1081 gccagtttcc acgctgccat catcagcgga taattccacg gtgcgataga agccacgacc
     1141 cccaacggat cgcgacggat catcgaagta tgaccttcaa gatattcacc tgccgccaga
     1201 ccattcagac agcgcgccgc acccgcgaaa aagcgaaaaa catcgacaat cgccgggatt
     1261 tcatcattga acgcactatg cagcggtttg ccacaattac gggactccag ttcggcaaaa
     1321 acctgaccat tttcttcgat aacatcagcc agtttcagca gacattccgc acgcactttc
     1381 ggcgtggttt gcccccattc ggcaaatgct gcatctgccg cgcgcacagc agcatcgacc
     1441 tgctctgcgg atgcctcggc aatctccagt aaaacgtccc ccgttgccgg attatagaca
     1501 ggctgttttt ccccttcgcc gctaaccagt tctccgttaa tcagtaactt atgttgcata
     1561 gcatattcct gtatcagtgg ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc
     1621 cccaggatcg gcaaggttgt taccaacata accagcagtg ccaccacgtt agttaccggt
     1681 acatcacgcg gtcgcccaag ctgattgagc aaccacaacg gtaacgttcg ttcatgacct
     1741 gccgtaaaag tcgtaacgat gatttcatcg aacgacaagg caaacgccag cattcctcct
     1801 gccagtagcg ccgaactgag attcggcaac actacgtagc ggaaggtttg ccagccattg
     1861 gccccaagat ccattgacgc ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg
     1921 acattgttaa acaccaccac tacgcaaaaa gtcgcatgac cgaccacgat ggtgaaaaat
     1981 cccggctcca gattgatggt tttaaaagcg gttaatagcg ccagaccagt gacaatgccc
     2041 ggcagcgcaa tgggcagcag cagtaacagc gaaatggcgt ttttgccgaa aaagtctcgt
     2101 cgccacagcg cggcagctgc cagcgtccct aacaccagcg ccattaatgt cgccagcgcc
     2161 gccactttaa gtgacagtgt cacggcatca agaatatcac tacgctgtgc tgccacgcta
     2221 aaccagcgca gcgtcaggcc ctgcggtgga aaactaaacg ccgcatcttc agtgttaaac
     2281 gcataagcgg cgataatcag gatgggaaaa tgtaggaaaa ccacgccgcc ccaggccgcc
     2341 agtttgagga aaaacggtgc gcgttcagag tgcatcgaac gctcccagac gtttcacgaa
     2401 cgccaggtac agtgcgatga gaacaatcgg caccagggtg aatgccgccg ccatcggcat
     2461 attgccaatc gccccctgct gggaataaac catattgccg ataaaatatc ctggaggacc
     2521 aaccagctgc gggacgataa aatcgcccag tgtgagtgag aaggtaaaga tagagccagc
     2581 ggcaataccc gggattgcca gcggcagcac cacatagcga aaggtttgtc gtggacgtgc
     2641 gccgagatca gccgacgcct gcaacaatga cggcggcaaa cgctcaagcg ccgcctgaac
     2701 gggcaggatc atgaacggca accagatata gagaaacacc agaaagcgcc ccagcccgga
     2761 agttgacagc gtatttccgc ccaccgcagg taatgtaagg aacgcagtca gcagtggttc
     2821 cagcccaaga tgttgtaaaa accactgagc cacgccatct tttgccagca ataacgtcca
     2881 ggcataggct ttaacaatgt agctcgccca catcggcaac attaccgcaa tataaaaaaa
     2941 agctttcatt ttcccgctgg tatagcgcgc catataccac gccattggaa aagccagaat
     3001 ggcgctggcg atagtgactg ccacagccat agtcagcgtg cggagaatga tgtcgtaatt
     3061 cgccggatta aacagcgcac ggatattcgc cagcgtcagt tccggcgtta ccgacatggt
     3121 gaaatcgtca aaagtataaa atccctgcca taacagtgtc agcagcgagc cgaaatagac
     3181 aatgccaaac cacattagcg ggccaagcag cagtaaaaac agccccagcc tcggattacg
     3241 ccagaagaaa ccggacacct tacccagacc tggacgtgaa ggtgattgca atacattcat
     3301 cgccattcac ctctcctcaa ccagcggcac catcacatca cgcgaccagg aaaccatcac
     3361 ctgttgtccg ggcgtgagcg tggcaggcag ttcttcgcct gtcatattgg cctgactcac
     3421 aagcagtttt tcaccgccgt tcaatttcag ttcaaaacga gttgccgcgc cctgatattg
     3481 caccgcctgg atcgtgccat tggcctgcag ttcaccaggg gtgttgaggc ggatatgttc
     3541 cggtcgcagg gcgaagcttc ccgtcatgcc acaaagtttc tctgccatca gtccatcaaa
     3601 aacgttggat gtcccgacga atccggcaac aaacggcgtg cgcgggcgca tatagagatc
     3661 gcgcggggaa tcgacctgct caatgcgtcc attattgaaa accgccacac gatcggacat
     3721 cgataacgct tcgccctgat cgtgggtaac gaagataaaa gtgataccga gagactgttg
     3781 cagttttttc agttccagct gcatctgctc acgcaatttg agatccagtg cgccgagcgg
     3841 ttcatccaac agcaatacgc gcggttcatt caccaatgct ctggcgatag caacccgctg
     3901 gcgctgacca ccagaaagtt gtgacggttt acgttgatgt acaaacccca acgccacttt
     3961 ctccagcgcc tcttgcgcca ttgcgtgccg ctgcttttta ttcacgcctt tgaccatcag
     4021 cccataggcg acattgtcaa gaatcgacat atgcggaaat agcgcgtagt cctgaaagac
     4081 agtattcacg tcccgctccc acggtggcag attgctggcg ggtttaccaa agatagagat
     4141 agccccgccg gaaagctgtt cgaagccagc aatcaggcgc aggcaggtgg ttttgccgga
     4201 gccggacggc cccagcatag agaagaactc accatctttt atcgcaatac tgacgccatc
     4261 tactgcgcgc acgtcaccgt acaaccgcga gacgttgtca aactccactg cgtacgtcat
     4321 aaagcacccc agcgaagtta gcgaccgccc ataatggcaa tgtaatcctg cgtccagcga
     4381 ctgtagggaa caaacttgcc cccttctgct ataggcgttt tccagaaggc gattttgtcg
     4441 aaatagttaa aaccgttggt ttcacaacct ttttcgccta ataacggact ggctttacac
     4501 ccttccggca ctaccggtaa cgagccaaac caggccgcca catcgccctg cacttttggc
     4561 gttaatgacc agttcatcca tttgtaggcg caaaccggat gtttcgcttc gctatgcagc
     4621 atggtggtat cagcccaacc ggtaacaccc tccttcggga aaacggtagc aacaggctgg
     4681 ccttcggctt tcagggcgtt ggcctgatag ggccaggcac tggaagcaac cacaccctcg
     4741 tttttgaaat cgctcatttg cacggtagtg tcatgccagt agcgatggat caaactgtgt
     4801 tgagcgcgca gcactttcag caccgcctgg tactgttctt cggttagttg atacggatcg
     4861 ctgatgccca actgcggctg agtggcttta acgaacaacg cagcgtccgc aatgtagata
     4921 gggccatcat aagcctgaac gcggccttta ttgctcttgc cgtccggcag attttgctca
     4981 acaaaaacca cttgccagct atccggcggc gtcgggaagg ttttagtgtt gtacatcagc
     5041 aggttcggcc cccattggta aggtgtgccg taaactttgc cgccaacatt aaaccagtcg
     5101 cctttaacca cgcgcggatc gagcgttttc cagttgggaa tcaaggcggt attaatcggc
     5161 tgcacgcgtt tgcccataat caaacgcagc gaggcatcgc cggatgccgt aaccagatcg
     5221 taaccccctt tggtcatcag actgaccatt tcatcggaag tcgcggcggt tttcacattc
     5281 accgcgcagc ctgtctcttt ttcaaattgc gttacccagt cgtattgttt atcagtttgt
     5341 ccgcgttcga tgtatcccgg ccaggcgata atatccagtc gcccttccgg tttatctaaa
     5401 ttggtaggtg gttcggcggc gtgagcggtc attattgtca tggtgagcgc acacaggctg
     5461 ctgcgggcaa atgtcttgct cataaggtct tactcctgtc gtaattaatt gttcagccgc
     5521 cgtgccgtaa aaatatctcc ttgtttaacc atagacggcg gcttgcaggc acaccttcgc
     5581 ccctgagaaa atttaatcag gttgtgagct accgcgcatt atgccgtctg taaggttatc
     5641 ttctggagta tagacaaggg gttaggaaat atgactgcta ttcgaaattc atattaccca
     5701 atctaagagg aattaaatta aacgctatta cagccgttct tgaataagtt tgcctaattg
     5761 ttttaccgcc tgttcttcac gttctcccca ctgccacgcg gtattaaaac gaaaaaaacg
     5821 tgaccagttt tcaccggtag aaaacatttt acccggcgca atactgatat gatgcgtcag
     5881 tgccgccagg cttaattcgc cggcatctaa cggctcgggg agctccagcc agagaaaata
     5941 accgctgtca ctatgatgaa ttttaacttc cgcaggcaga taacgcagca gtgcctgcca
     6001 ggcacgttgt ttacgttccg caagctggcg acgcaggcga cgaagatggg cgtcgtatcg
     6061 gcgcgtggaa aggtaatcca ccagcgcaag ctgcatcggt gagctggtgg aaagcgtact
     6121 catcaactgc aagcgttgaa ttttacgtgc atgttttccg gcggcgaccc aaccaatacg
     6181 aaaaccaggc accagacatt tcgaaaacga agagcaatgc aaaacgccat cgtggcgatc
     6241 ccacgctttc gcaggcagcg gtttttcccg tccaaaataa agttcgctgt aaacgtcatc
     6301 ttcaatcagc gttacgttgt actgattgag taacgccacc agttgtgctt tttttgcggc
     6361 gttaaggtaa atccgagtgg attctggcta ttagtcatca gccagcacgc tttcaccgga
     6421 tactcctgca acgccagttc caacgcctga agatctatcc cttctttaac atccgtcgcc
     6481 accgataacg ctttcagccg tagccgttcc agcgcctgca acgcgccata gaaacaagga
     6541 ttctctacta tcacccaatc gcccggttca gttaccgctt gcaaactgag gtttaatgcc
     6601 tctaacgccc cggcagtaat gacaatttca tcaggagaaa tggtgatgcc ctgtaaggca
     6661 tagcgacgag caatagcctg acgcagttct gcgtttcctg gcggtaagtt ttcaatcacg
     6721 ctcatcgccg tggcggtttt gcttacctgc gccagcgagc ggtttagttg ttggaggggg
     6781 aaaagtcgcg ggtcgggaaa ggccgaggca aacggaacga ccgacggatc gcgactggcc
     6841 tgcaacatat caaaaatata agtgttgata tcgactgctt catctcgagt gactggaatg
     6901 actggcgctt tcggcatttt tattgcctgt ggcgcaacgt aataacccga ctgcggtcgt
     6961 gcgataatat atccctgact ttcgagcaac tgataggcat ggctgacagt cataaagctc
     7021 atgcctgaaa gcgccacctg gtcacgcaac gaaggcaaac gatcgccggg ttgccagata
     7081 cccgacgcaa tctgctcgcg taattgttct gcaagctgct ggtatttttt cattatcgaa
     7141 tcgtaattat gtgcagatga ttcggcagtc tatatcagtt gttaaaaatg acaacttttc
     7201 gttaactgta attaaaccat caccagcgat aactctttgc caagcgcctt tgccgcaagc
     7261 tggacggcgt cgatttttgt cgcatggtgc aagttaaata ggcgagtaat ctcctgttta
     7321 ggtttgccaa ttcgcctggc taactcttgc tgagtgattt ctgactgtaa aaaagcattt
     7381 aacagcaata ctttagaggc gacgctcaaa ggtacttcaa taaagtgatc atgactattt
     7441 aatggcgaag gtaacgggat aagctcgtta tcttcaaaat aaaaatcaaa tgcggtcagt
     7501 aaagcatctt ttgccgcttc catcgcttca gcgacagttt cgccctgcgt caacgcttca
     7561 gggatatcca caaaagaaac catataaccg ccttccggcg cgggtgtaag agtgacggga
     7621 taacgcataa tttcgactgt ccctcgcatg tcattaacct ttttaattgg cgattaactc
     7681 aaaccgagtt gtttcaggat tgctttacgc aatggttctt taatctcatc gcaggggtga
     7741 cgcggcatga cactgcgcct cccatgaaac ctgagtttca aatggttgct gccattcgct
     7801 acatcgacgc cctgagattc gagccaacgt ctgaactcgc tttgtttcac atcgcctccc
     7861 tgccgctgag tatatcaaca aagtaaacat tagtgtttac ctccgtgtgt aatttgctcg
     7921 aaaatagcac taaatcgccg caacggcaga atgccatgca aaatcaggaa gaaagactcc
     7981 agaatttctt tgcgctaatt tgcaaaactg aataataacc tcaagaaacg atcggcatca
     8041 tagcggcaaa aggtaagaaa aaggtggcag gatgtttacg aaggcgttat cggttgtctt
     8101 attaacgtgt gcgctgttct caggacaact catggcaggg cacaaaggac atgaatttgt
     8161 gtgggtaaag aatgtggatc atcagctgcg tcatgaagcg gacagcgatg aattgcgtgc
     8221 tgtggcggaa gagtcggcgg aaggtttgcg cgagcatttt tactggcaaa aatcgcgcaa
     8281 accagaagcg ggacaacgtt gagtcatttg ttgccggatg cggcgagacg ccttacccgg
     8341 caggcaatat catgcagatt caatgagttg tgagtaacca gaaaactggc gttttaccct
     8401 tttcgcttag gcaatgtttt cagcagctca tccggactta cggatgccac tacgcttccc
     8461 ggtaacggca ttttcagtat gtgatttttg attttgccaa tgacgtgcat ctcgcatggg
     8521 cggcaatcaa actttagcgt taatacttca tcgccgttaa ccagttgcat tggcgtcgcc
     8581 ttccagcttt tgatattgcc tttcgcctgt ttcgggcaca gattaaaggc aaagcgcaga
     8641 caatgcttgg tgatcatcac cgggacttcg cccttctctt catgtgcttc atacgccgcg
     8701 tcaatcagct gcacaccata gcgatgataa aattcacgcg ctttctggtt gtatacgttc
     8761 gcgaagaaac tcagatgcgt ttgcggataa accggcgcag gatcagcaac cggtttacgg
     8821 ctgccgcgct ggtaactggc aagacgcgca gcatccagca tgtcagcagc ttcacggcgg
     8881 aactggttta acagactgtt tggtacaaac agcgcccccg gcaaattaat ttgcacatcg
     8941 cgggcgtaat agagggtttg ccccagtttt gccagaccat ccttcagatt gttcattgct
     9001 ttttcggcgt tattggcttc gtcgaactgc ccgtccagcg tatgcgtgat gctgacaccc
     9061 tcttcactgg tgagggtcag aatcagttgt tcctgccagc cgcccagttc aatgtctacc
     9121 gccacccgac gttcgctgga ggtttttgtc agtgcctgct gccagttatg atcaagatta
     9181 cggtttagtg ggtgatgtgg acgaattttg tgcaaatctg ctggcatttc attgggccag
     9241 acgcggtact gattttctcc ggttttctcg accgtattgg cacgaaaacc gacgacttca
     9301 cgtttaatca tcacgttcag gccatcacca tttgccagtg gctcggtgac ggcaacatcg
     9361 agatgatctt tcgccacttt caatacttcg cctaccggca ggccgataaa tttcggcgaa
     9421 tcgaacgcgc caatatcgcc tttacgggca ttcacaaaat aatctgtgct accacggtgg
     9481 aaagtctttt ccgtcgatgg aacaaagaaa tgttcagtac gacctgatga agcgcgcgcc
     9541 agatcgccac gttcttcaat aatggcatca agcatctggc gataatgggc ggtgatattc
     9601 ttcacgtagc tcatatcttt gtaacgccct tcaatcttga aggagcgtac gccagcatca
     9661 atcagcgcgc cgaggttggc agtctgatcg ttatctttca tcgacagcag atgtttttca
     9721 taggaaacca cccgcccctg atcgtctttc aatgtgtatg gcaaacggca cgcctgcgag
     9781 caatcaccac ggttggcgct acgccctgtt tgcgcatgag aaatgtagca ctgacccgaa
     9841 taggccacgc acagtgcccc atgaataaag aattcaatgg tcgcgtccgt ggcctggtga
     9901 atcgcgcgga tctgctcaag attcagctct cgcgccagca caatctgcgt gaagccaaca
     9961 tcagagagga acttcgcttt ttctactgta cgaatgtcgc actgcgtact ggcgtgcagt
    10021 tcaatcggcg gaatatcaag ttccagaatc cccatatcct gaacaatcag cgcatcgaca
    10081 ccggtctggt agaggtcggt aatcagccgt tgcgcgggtt ccagctcatc atcatgcaaa
    10141 atggtgttaa gcgtgacgaa aatttttgca ccataacgat gggcaaacgg caccagctcg
    10201 gcaatatctt tcaggctatt actggcatta tgacgggcac caaaaccaga gccgccgata
    10261 taaacagcat cggcaccgtg caaaatagct tcgcgggcaa tggcggcatc gcgtgccggg
    10321 cttaacagtt caagtcgatg agaagatacg gtcacattct tatccagtag ctgaaaaatg
    10381 gcggctattt tagccacaag cgcgacgttg cgaaatagtt ttcccgctta actgcgcggg
    10441 taatggatga gggaatgaaa atgtacggtt tgctccccac cattacgata gatatgcgtg
    10501 acgtctgcag caaatcgaac gccttcccca caattaaggg tctgccattc cccatcaacg
    10561 cacagatcaa gttgtccatc aatgacgacg acatgttcga ttaccccctt ctcatggggc
    10621 gtcgattcgc ttattgcacc agatgccatc tgaatcgaga agtgttcgaa gcagagttgt
    10681 ggatcataag gaaacagcga agtgatcacc atcgcctgct gttgtggatc ataaactgaa
    10741 ggcgtagcgg actgcggcgg agaaataaat gttgaaaatg gtacgttcaa cccggtggca
    10801 attttccata atgtcgcgac cgtcggactg gactcattac gctcaatctg ccctaacatt
    10861 gctttagaaa cgcccgtcgc ttctgccagt cgcgaaagac tccagccgcg ctgctggcgt
    10921 agttgtttaa gtgtggtgga taaaaagcga gcgagatttt ccatgcgact cctccggcaa
    10981 aacggaagtt tatcacttgt gcgttataac ggacaaatgc tacggtgcct gtacgctata
    11041 acgcacgagg tgactatgcg tctgttttct attcctccac ccacgctact ggcggggttt
    11101 ctggcggtat taattggcta cgccagttca gcggcaataa tctggcaagc agcgattgtc
    11161 gccggagcca ccactgcaca aatctctggc tggatgacgg cgctggggct ggcaatgggc
    11221 gtcagtacgc tgactctgac attatggtat cgcgtacctg ttctcaccgc atggtcaacg
    11281 cctggcgcgg ctttgttggt caccggattg cagggactaa cacttaacga agccatcggc
    11341 gtttttattg tcaccaacgc gttaatagtc ctctgcggca taacgggact ctttgctcgt
    11401 ctgatgcgca ttattccgca ctcgcttgcg gcggcaatgc ttgccgggat tttattacgc
    11461 tttggtttac aggcgtttgc cagtctggac ggtcaattta cgttgtgtgg aagtatgttg
    11521 ctggtatggc tggcaaccaa ggccgttgcg ccgcgctatg cggtaattgc cgcgatgatt
    11581 attgggatcg tgatcgttat cgcgcaaggt gacgttgtca caactgatgt tgtctttaaa
    11641 cccgttctcc ccacttatat tacccctgat ttttcgtttg ctcacagcct gagcgttgca
    11701 ctcccccttt ttctggtgac gatggcatcg caaaacgcac cgggtatcgc agcaatgaaa
    11761 gcagccgggt attcggctcc tgtttcgcca ttaattgtat ttactggatt gctggcactg
    11821 gttttttccc ctttcggcgt ttattccgtc ggtattgcgg caatcaccgc ggctatttgc
    11881 caaagcccgg aagcgcatcc ggataaagat caacgttggc tggcagctgc cgttgcaggc
    11941 attttttatt tgatcgcagg tctgtttggt agtgctatta ccgggatgat ggctgccctg
    12001 cccgtaagtt ggatccagat gctggcaggt ctggcgctgt taagtaccat tagcggcagt
    12061 ttgtatcagg cgctgcataa tgagcgtgag cgcgacgcgg cggtggtggc atttctggta
    12121 acggcaagtg gattgacgct ggtcgggatt ggttctgcgt tctggggatt aattgccgga
    12181 ggcgtttgtt acgtggtgtt gaatttaatc gctgacagaa accgatattg acatcctcca
    12241 cgccctgaag gacgaggatc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg
    12301 ttcctgcttc aacgggcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
    12361 cgccgctaaa atgttacgag cgccatttac atcggcgttc gctgtatatc cacatacctg
    12421 gcatctgaat ttactttgtg acaggcggtt ttctttcgct gtatgaccac agcacgcgca
    12481 acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccacgcc agagctgctt
    12541 gtactcaagc tggcggcgca tttcatacca gccctgatcc agtatcgaac ggtttaaacc
    12601 tgattttgcc cggacattgc gccccggctg gctgaccgta cccgctgctg actttgacat
    12661 gttgctgacc ttcaaatcct caatgactat cattgcgtgg tttttgctga cggtcgttgt
    12721 gactttgtga aggtagtccc tgcggatatt tgcgatacag gaatgcagtc gctgtatttt
    12781 gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg cggcttaact gtcgctgaag
    12841 tctcgccagc gtcttctggt ttttctgaaa actgtttaca ggctcaaaga ctgtgccatc
    12901 tgacagcgtg gcgagtttag ccacgccagc atccagcccg accattgatg ctgaagggtg
    12961 agccggagtg gatacttcac tttctgtctg aatactgatg taccacttac cgcaggactg
    13021 gctgacagtg acatttttca caacacccgt gacctgacgg cta
//
LOCUS       DAZFDP010000134        12537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFDP010000134 DAZFDP010000000
VERSION     DAZFDP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            281..620
                     /locus_tag="ACHJ1G_003886"
                     /pseudo
     CDS             281..620
                     /locus_tag="ACHJ1G_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(663..1660)
                     /locus_tag="ACHJ1G_003887"
                     /pseudo
     CDS             complement(663..1660)
                     /locus_tag="ACHJ1G_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611812.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(1479..1595)
                     /locus_tag="ACHJ1G_003887"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(2046..3239)
                     /locus_tag="ACHJ1G_003888"
     CDS             complement(2046..3239)
                     /locus_tag="ACHJ1G_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4765660.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            3375..5099
                     /gene="iucA"
                     /locus_tag="ACHJ1G_003889"
     CDS             3375..5099
                     /gene="iucA"
                     /locus_tag="ACHJ1G_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO4765661.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGGCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEL"
     gene            5100..6047
                     /gene="iucB"
                     /locus_tag="ACHJ1G_003890"
     CDS             5100..6047
                     /gene="iucB"
                     /locus_tag="ACHJ1G_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO4765662.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            6047..7789
                     /gene="iucC"
                     /locus_tag="ACHJ1G_003891"
     CDS             6047..7789
                     /gene="iucC"
                     /locus_tag="ACHJ1G_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO4765663.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7786..9123
                     /gene="iucD"
                     /locus_tag="ACHJ1G_003892"
     CDS             7786..9123
                     /gene="iucD"
                     /locus_tag="ACHJ1G_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO4765664.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            9129..11324
                     /gene="iutA"
                     /locus_tag="ACHJ1G_003893"
     CDS             9129..11324
                     /gene="iutA"
                     /locus_tag="ACHJ1G_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO4765665.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11582..11797)
                     /locus_tag="ACHJ1G_003894"
                     /pseudo
     CDS             complement(11582..11797)
                     /locus_tag="ACHJ1G_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            11884..12309
                     /locus_tag="ACHJ1G_003895"
     CDS             11884..12309
                     /locus_tag="ACHJ1G_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4765666.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            12306..12380
                     /locus_tag="ACHJ1G_003896"
                     /pseudo
     CDS             12306..12380
                     /locus_tag="ACHJ1G_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         2997 a         3260 c         3266 g         3014 t
ORIGIN      
        1 aacggggggt agatgaaagt gaatggtgtt actacatcaa aatatgaagg atggcgaggg
       61 acgtttcagt tatatagata taaaacggta gttctggtgc accccaaagg atatctggca
      121 ggggaaatga ttcatcgcca ggccctaacg tcgtcttgtc aagattaatg ctatagacat
      181 agctaaattt tttatggata aggatatgaa ctgtgcctca ggtccggaac atcctgtagg
      241 gactttttaa gtcgggattg agctgaacta atgccaaatt atgaacaggg aaattcagca
      301 gcggctacag tgggtaaaac tctatgaaac gtccggtgat gcgggatttg tatgccgtcg
      361 ctgtggcatt tctcggccca cattgcgtaa aatggtggcg cagatatctg gctcagggca
      421 tcgctggtct ggaaagccac agccgtcgcc ccaagcgttc accatcaacg aaaaccggca
      481 cttgcgaagt cgctctgatt ctggagttac gttctcagcg aaatctgggg gcaagacgta
      541 ttcaaagcga gttaaaacga cttcattcaa tctcactggc aatagcaaca atccacaaag
      601 tccttttcca gaatcaggtt tgaaccgccc cgggtttcct ggagagtgtt ttatctgtga
      661 actcaggctg ccagatcatc gtttccgatg gaagcataat aagctttttc tgcttctgcc
      721 ggaggagtat ggcccagcct ttccagcaat cgtcgattgt tataccagtc cacccacgtg
      781 agtgtggcca gttccacttc tgcacggttt ttccagctct tacggtgtat tacctccgct
      841 ttgtaaagac cattgatgct ctccgccatc gcgttgtcat acgagtcgcc tgtacttcct
      901 gttgatgcca gtaatccggc ttccttaagc cgctgtgtgt aggccagcga tacatactga
      961 gaacctttat cactgtgatg gaccgtgccg gacggtcgac gggcccataa cgcctgctcc
     1021 agtgcatcca gcacgaatgt cgtctccatg gacgatgaga cccgccaccc cacaatgtat
     1081 ccggcaaaca catcaatgat gaacgccaca tagacgaagc cctgccatgt gctgacgtaa
     1141 gtaaaatcag ccacccacag ctggtcaggt cgttctgcca cgaactgacg gtttacgcgg
     1201 tcgcctgcgg caacggcttt ccggctgatg gtcgtacgga cctttttacc ccggagaaca
     1261 ccggcaagtc ccataactgc catgagacgt gccacagtgc atctggccac tctgatacct
     1321 tcccgtaaca actgacgcca gactttacgc acaccgtata cctggtgatt ttcatcgtat
     1381 acgcgctgta tctctttctt cagccagtca tcgtgctgcg cacgggcact gcgtttatcc
     1441 ggatgatgtc gctgttgctg acaatggtaa tacgttgacg gagcaatatg cagttcgctg
     1501 cataccggtc tgaccccgta ctgctcacgc agcttatcca gcagtggcat cattttttcc
     1561 agaggcggtc gaactccgcc ttcgcaaaat aagcggaagc ctggcgaagg atatcgttac
     1621 tgcggcgcag ttcacgattt tcacgctcca gctctttcag gtgtcaacgc cactctgaga
     1681 gagttttatg catcccgtat tgggcactat gatgccggaa atctgcgagg ctcgacacct
     1741 ctctgatttt cccctttatt tatcacggtg ccccagatct aatgttgggg atgcgttcat
     1801 ctcattccca atgagtaatc gcactgttct cattgtctgc aggcatccgg acagagccat
     1861 gtgatgttgc actcacaacc agcatgactt caatgcaact taagtgacac cggccggttt
     1921 tataccgaac ataccctgta ccatgcggtg catttttgcc agtgttactg cctgacatgc
     1981 tttcatcaga cgcaggttct ctctcatatc ctctgagtta ctccggtaag gtgatgatca
     2041 tatctttatt tgatagcacg gtaatgccag ataaatacag acacaatggt aaataatata
     2101 gttgcaaata atgccgccat aatcagcgcg attgcgacac ctgttttcga taccagcggc
     2161 accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca gcccgcgcct
     2221 gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc cagagctgct
     2281 ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga gagcatgata
     2341 gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg agtcaggcgc
     2401 tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag acccaacagc
     2461 caggccacct gttggctaat ggcggtggta tccgtggcga actggcgagt aagggcaggc
     2521 gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca aagcagataa
     2581 ggcagacaat cccgcttcag actgacgctc ttgcgctccg gtgtgggctg tggtggtgtg
     2641 ccgggcaacc gcagcagcat cagcagcgcc agcactgggg ctgccatcag cagccccagt
     2701 ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa tagcctgccg
     2761 caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc tcctgctctc
     2821 tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc gtaggcgatc
     2881 cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt agccatcagc
     2941 acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc ccacaacacc
     3001 acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga tggcagaaac
     3061 agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt tgtctgtatg
     3121 accagaacag ggagcacaac cagcagaccg ttctgcccga taccgagaag cccggcactg
     3181 aacgcaagtg gccagcagga cagtggtttt tggggcgtat cttcgatccc aggtgacatt
     3241 ttaatgtttc aactccatgt attaattgtg tttatttgta aaattaattt atctgacaat
     3301 aacatttctt attgataatg agaatcatta ttgacataat tgttattatt ttactgtgtg
     3361 ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt
     3421 gcttcctgaa tgcactgatt cgtgaaacta cagactggaa actgacagaa tacccgccag
     3481 acgaattgat catcccgctg gatgagcaga aatcgctcca tttcagagtg gcttatttct
     3541 ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt
     3601 atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgg catcaactgt
     3661 ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc cacgcccata
     3721 cgcaacaggc aattgatgcc cgtcatgact ggaccgccct gcgtgaaaaa gcgttgaatt
     3781 ttggcgaagc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg cctaagtctc
     3841 atgaaccgtt taaccggcgg gaggctgaac gctacctgcc tgatatggca ccccacttcc
     3901 cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg catctcaacc
     3961 ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc aacgaattaa
     4021 gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg
     4081 gggggtgcca ggcacttgtt gctaaggggc tgattaaaga cttaggtgag gccggcacgt
     4141 cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc gatatgatca
     4201 agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg aaagaagtga
     4261 agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc
     4321 gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc gatcttaacg
     4381 gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga
     4441 aaagccagac caacgtactg gtctccctga ctcaggccgc acctgatggc ggtgattcgc
     4501 tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg caacaggccg
     4561 cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt acggctgaag
     4621 cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag atgcttgggg
     4681 atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg cctcacgcga
     4741 cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc catgagcagt
     4801 tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg accgctgcgc
     4861 tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga gcatcgctgg
     4921 ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt
     4981 actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag aacaccattg
     5041 ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag gagctctgaa
     5101 tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc
     5161 ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc ggggagctgc
     5221 cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca caactgtcag
     5281 cagtggctct cccctgggcc gaatggtgtg aggagccaca ggcgctgacg ctttttgggc
     5341 aggtaaaaag cgacattatc catcgtaccg ccttctggca gttaccgtta tggttgagtt
     5401 caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag atttatttcc
     5461 cgcagcgccc accccgtccg cagggtgagg tttaccgccg ttacgatcca cgtattcgca
     5521 ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc acccgctgga
     5581 tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag gtacagaccg
     5641 cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg tgcttcgacg
     5701 atcggccatt cagctatttt gaaatctact gggcggcgga agaccgcatt ggtcgtcact
     5761 attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag caatggcgcg
     5821 gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg ctggatgagc
     5881 cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg ttccgccatc
     5941 ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag cgctcgcgca
     6001 tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga atcacaagga
     6061 ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg agtatgagca
     6121 ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc cgggagcaca
     6181 atggcgcttt atcgctgaac gtggtatctg gggctggctg tggattgatg cccaaacgtt
     6241 gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa agccggtgct
     6301 gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca cgctactggg
     6361 cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga ttaatcttga
     6421 tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg
     6481 ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatgcca acaccttcag
     6541 actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgaca acgatctgga
     6601 tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct tcagtcaggt
     6661 ctggcaggaa aacgggctgg atcacaactg gctgccgctg ccggtacatc cgtggcagtg
     6721 gcagcaaaaa atcgccaccg acttcatcgc tgattttgcc gaaggcagga tggtgtctct
     6781 cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga ccaacgccag
     6841 ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct catgctaccg
     6901 ggggatccct ggcagatata tcgctgccgg accactggct tcacgctggc tgcaacaggt
     6961 ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg aaccggctgc
     7021 aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc gctaccagga
     7081 aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc
     7141 ggttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg caggcgcata
     7201 tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc gggtggttgt
     7261 ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac acggacaaaa
     7321 tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag acttccaggg
     7381 cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc aggaggtgcg
     7441 tgatgtcaca tcccgtctga gtgcggacta cttaatccat gatttgcaga cgggtcactt
     7501 cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac ctgaaaggcg
     7561 attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc cacaaatgtc
     7621 agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg tgctgaaccc
     7681 ggtaaaactg acctggccgg accaggatgg cggcagtcgc atgctgccga attaccttga
     7741 gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc
     7801 gattttattg gtgtcgggac agggccattt aatctcagta ttgctgcatt gtcacatcag
     7861 atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg gcatccgggt
     7921 atgctggtac cggattgtca tatgcagact gtctttctga aagatctggt cagtgcagtt
     7981 gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa gttctatcgc
     8041 ttccttacaa gcagactacg tacagtatcc cgtgaagagt tttctgacta tctccgctgg
     8101 gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat tgatttcgac
     8161 aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc ccgcaatatc
     8221 tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt gacacaatcc
     8281 tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa acggataact
     8341 gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg cggggaatgg
     8401 ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc actggatgag
     8461 gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc cggactgaag
     8521 gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg catcactgcc
     8581 gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct gagaaaacca
     8641 agaaatatcc gtctgctacc cagccgctcg gtaacaactc tggaaagtag tggtccgggc
     8701 tggaagttat tgatggaaca tcatctggat cggggcagag agagcctgga aagtgatgtg
     8761 gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc actgatgccc
     8821 ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac tctggaatgg
     8881 agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac tcatggcatc
     8941 gccgaacccc agctcagcct gatggcctgg agatctgcac gtattcttaa tcgcgtactg
     9001 gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg cagcggcagc
     9061 cggaaaaaac cgcagccgga ggctgctgct ttaactcact atacaacaaa tattcaggaa
     9121 taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc
     9181 tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct gccagccgca
     9241 gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat gccgaactgg
     9301 agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta atccccggcc
     9361 ttgatgtcag cagccagagc cgaaccaact acggtatgaa catgcgtggc cgcccgctgg
     9421 ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga caactggact
     9481 ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg gccctgtacg
     9541 gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggccag ccggaaacca
     9601 tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat cacgatgagc
     9661 gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt tccgtggcat
     9721 atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt gataacaccc
     9781 agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg ctgaacatcg
     9841 atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcagggg gacgacaatt
     9901 acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca ccatacgtca
     9961 gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc agcctgcagt
    10021 actctgacag tgatttcctg agacaggaac tggtcggtca ggtttactac cgcgatgagt
    10081 cgttgcggtt ctacccgttc ccgacggtaa atgcgaataa acaggcgacg gctttctcct
    10141 cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc caacttatgg
    10201 acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc tccaaccaga
    10261 tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat aagatttaca
    10321 ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg caatccagct
    10381 atgacattaa tgatattttt accgtcagcg gtggcgtacg ctatcagtat actgagaaca
    10441 gggtagatga ttttatcgac tacacgcagc aacagaagat tgctgccggg aaggcgatat
    10501 ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc aatgctggtc
    10561 tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag ggggtggcat
    10621 tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg aacggccatc
    10681 ttcccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt
    10741 cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc gcagcatatt
    10801 actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt gtgaaggacg
    10861 acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg gatactgact
    10921 ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc
    10981 aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt agctgggcgc
    11041 cggaaccgtg gagtctgcgt gtgcagagca ccacttcttt cgacgtaagc gatgcagagg
    11101 gtaacgatat taatggttac actaccgtcg attttatcag tagttggctg cttccggtgg
    11161 gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact gtctggggac
    11221 agcgtgcacc tctgtactac agcccgggtt acggccctgc ttcactgtac gactacaaag
    11281 gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga ttcttttgca
    11341 acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca gccagtataa
    11401 tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact gaataacaat
    11461 atgtctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc attgtccgtc
    11521 tgaaaactaa ccttacaagt gaaccgcccc gggtttcctg gagagtgttt tatctgtgaa
    11581 ctcaggctgc cagatcatcg tttccgatgg aagcataata agctttttct gcttctgccg
    11641 gaggagtatg gcccagcctt tccagcaatc gtcgattgtt ataccagtcc acccacgtga
    11701 gtgtggccag ttccacttct gcacggtttt tccagctctt acggtgtatt acctccgctt
    11761 tgtaaagacc attgatgctc tccgccatcg cgttgtcagt aagcgtaaac tgaccgccgt
    11821 atgtagccat cagacgaaaa ttggtaactt agacgcccat ctgatataga cggacatcta
    11881 agtatggaat tacaggactg gcgaaaagaa cctcgtaaaa actattcgaa tgaattcaaa
    11941 cttcgtatgg tggaactggc atcacaacca ggagcctctg ttgcccgtat tgcccgggaa
    12001 cacgatatca atgataacct cctgttcaaa tggctcaggc tctggcagaa cgaagggcgt
    12061 atatcacggc gtcttcaggt aacgacctct tctgacactg gcgttgaatt attacctgta
    12121 gaaataacgc cggatgagca gaaagaaccg gtggcggcca ttgcgccgtc tttatccact
    12181 tccactcaga ccagtgtcag tgccggctcc tgcaaggtgg agttccgtca cggtaacatg
    12241 acgctggaaa atccatcgcc agagctgctc acgctgttga tccgtgaact gaccgggagg
    12301 ggacgatgat ctcactcccg tcaggtaccc gcatctggct cgttgccgac gttaccgata
    12361 tgcgtaaatc cttcaacgga tacgacatat gctgaaggag gagcagtaat ggattaagtg
    12421 ataacaaata cccagaaata gaggttcaaa tccagcttag ttagtacata aatactattt
    12481 tgatgaaaat tattatatgt ttcctcagat tttaatcagg cgcccagatt aaaccaa
//
LOCUS       DAZFDP010000135        12481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFDP010000135 DAZFDP010000000
VERSION     DAZFDP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(21..302)
                     /locus_tag="ACHJ1G_003897"
     CDS             complement(21..302)
                     /locus_tag="ACHJ1G_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO4765667.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(565..1674)
                     /gene="apbC"
                     /locus_tag="ACHJ1G_003898"
     CDS             complement(565..1674)
                     /gene="apbC"
                     /locus_tag="ACHJ1G_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4765668.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1806..3839
                     /gene="metG"
                     /locus_tag="ACHJ1G_003899"
     CDS             1806..3839
                     /gene="metG"
                     /locus_tag="ACHJ1G_003899"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4765669.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3980..7774
                     /locus_tag="ACHJ1G_003900"
                     /pseudo
     CDS             3980..7774
                     /locus_tag="ACHJ1G_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7784..11416
                     /gene="yehI"
                     /locus_tag="ACHJ1G_003901"
     CDS             7784..11416
                     /gene="yehI"
                     /locus_tag="ACHJ1G_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4765670.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11478..11795
                     /gene="yehK"
                     /locus_tag="ACHJ1G_003902"
     CDS             11478..11795
                     /gene="yehK"
                     /locus_tag="ACHJ1G_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4765671.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3269 a         3165 c         3061 g         2986 t
ORIGIN      
        1 attaaaatca ggttgccaaa ttaatcgtag cgatttaaca cgattcgacg tttgctatcg
       61 ctacctgcaa caaccacttc tgtcgttaca cctttgacgg gcgaagaaaa cagatgacgg
      121 gtgtcaacat tatacgttaa ctgaccacaa tgctgttgag gttgaccatt aaccatagcc
      181 atactgcatg ttgggggact gatcctgcca ttaatcgcta atgtggcagt ctctgaagag
      241 aatgcgggag aagccaatcc ataggcaaga aaaataattc ctgacagcca atacttattc
      301 attgaacgtt atccctgtag taaaggttat gcctggatag aatgagtgca taacaaacta
      361 tagctgtaca tccactacac agccacgaag gatgataatg aagcattgcc tgtatgatca
      421 atcgactttg tagagtttcg gacgaaggtc cgcagaatat tcgcagtatt aaataagtct
      481 tcattaatgc ggcaaaaact aatacaccgc atcaatgtaa catctctgcg gtgtatgaag
      541 aataaccaga atggttaata ggcgttagac cgcgcggaag gaaatctcgc ctggaatgac
      601 ttcaccctgc cagtagagct gagctgcaac gcggtcagcc agctggcggt agatagcggt
      661 aaattcgctc tccggacggc tgataaccgt tggcgtgcct ttatcgagat cctcacgtaa
      721 ggagatgtgt aacggcatct gccccagcag ctgtgtgtgg tatttttcag ccagtttctc
      781 agcaccaccg gtaccgaaaa tcggttcatg gtgaccacag ttactgcaaa tatgcacact
      841 catgttctcg acaataccaa gcaccggaac ctcgactttc tcgaacatca caatgccttt
      901 tttcgcatcg atcaacgcga tgtcctgcgg ggtggtgacc acgactgcac cagttactgg
      961 aatgttttgc gccagcgtca attgaatgtc accggtgcct ggcggcatat caagcaccag
     1021 atagtcgaga tccggccaca aggtttcctg caacatctgc attaacgcct tgctcgccat
     1081 cggtccacgc cacaccatcg cattgtcgtc agtgaccaga taaccaattg aattggttgc
     1141 caggccatga gacataatcg gtgccatgtg ggtaccgtcc ggcgacgttg gacgctgatt
     1201 ttctgcgccc agcatggtgg gaattgatgg gccgtagata tcggcgtcca gaatgccgac
     1261 tttcgcccct tccgccgcca gtgccagcgc caggtttacc gccgtggagg atttacccac
     1321 gccgccttta ccggaactga ccgcaatgat gttcttcaca ccattaatac ccggctgatt
     1381 cttcacgcgt tttaaggtgg cgatattgtg cgacagtttc cagtcgatag ctttcgcccc
     1441 ggtgatacgc agcagttcgg cgctacattg ctcttttaac tcttcaaacg cgctatgcca
     1501 gacgaatggc ataaccagtt ccacatgcaa cgtgtcgtcc atccaggcga catggtgcaa
     1561 cgctttcagc gtggtgaggt tatgcttcag ggttgggtgc tgaaaattgg ccagtgtccc
     1621 ggcgaccatt gcgcgcaagg cttctggtga tttggcctgg gattgttcgt tcatcccgac
     1681 tccttttttg tatagataaa ccatcagctg atagtttacc tgaagaatat agagaagtac
     1741 ttacttaaca ttttcccatt tggtactatc taaccccttt tcactattaa gaagtaatgc
     1801 ctactatgac tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg tacgctaacg
     1861 gctcaatcca cctcggccat atgctggagc acatccaggc tgatgtctgg gtccgttacc
     1921 agcgaatgcg cggccacgag gtcaacttca tctgcgccga cgatgcccac ggtacaccga
     1981 tcatgctgaa agctcagcag ctcggtatca ccccggagca gatgattggc gaaatgagtc
     2041 aggagcatca gactgatttc gcaggcttta acatcagcta tgacaactat cactcgacgc
     2101 acagcgaaga gaaccgccag ttgtcagaac ttatctactc tcgcctgaaa gaaaacggtt
     2161 ttattaaaaa ccgcaccatc tctcagctgt acgatccgga aaaaggcatg ttcctgccgg
     2221 accgttttgt gaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac ggcgataact
     2281 gcgaagtctg cggcgcgacc tacagcccga ctgaattgat cgagccgaaa tcggtggttt
     2341 ctggcgctac gccggtaatg cgtgattctg aacacttctt ctttgatctg ccctctttca
     2401 gcgaaatgtt gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg gcaaataaaa
     2461 tgcaggagtg gtttgaatcc ggcctgcaac agtgggatat ctcccgcgac gcgccatact
     2521 ttggttttga aattccgaac gcgccgggca aatatttcta cgtctggctg gacgcaccga
     2581 ttggctacat gggttctttc aagaatctgt gcgacaagcg cggcgacagc gtaagcttcg
     2641 atgaatactg gaagaaagac tccaccgccg agctgtacca cttcatcggt aaagatattg
     2701 tttacttcca cagcctgttc tggcctgcca tgctggaagg cagcaacttc cgcaagccga
     2761 ccaacctgtt tgttcatggc tatgtgacgg tgaacggcgc aaagatgtcc aagtctcgcg
     2821 gcacctttat taaagccagc acctggctga atcattttga cgctgacagc ctgcgttact
     2881 actacactgc gaaactctct tcgcgcattg atgatatcga tctcaacctg gaggatttcg
     2941 ttcagcgtgt gaatgccgat atcgtgaaca aagtggttaa cctggcctct cgtaatgcgg
     3001 gctttatcaa caagcgtttt gacggcgtgc tggcaagcga actggctgac ccgcagttgt
     3061 acaaaacctt cactgatgcc gctgaagtga ttggtgaagc gtgggaaagc cgcgaatttg
     3121 gtaaagcagt gcgcgaaatc atggcgctgg ctgatctggc taaccgctat gtcgacgagc
     3181 aggctccgtg ggtggtggca aaacaggaag gccgcgatgc cgacctgcag gcaatttgct
     3241 caatgggcat aaacctgttc cgcgtgctga tgacttacct gaagccggta ctgccgaaac
     3301 tgaccgagcg cgcagaagca ttcctcaata cagaactgac ctgggatggt atccagcaac
     3361 cgctgctggg ccacaaagtg aatccgttca aagcgctgta taaccgcatc gatatgaagc
     3421 aggttgaagc actggtggaa gcgtctaaag aagaagtgaa agcagctgcc gcgccagtta
     3481 ctggcccact ggcagatgat ccgattcagg aaaccatcac ctttgacgac ttcgcaaaag
     3541 ttgacctgcg cgtggcgctg atcgaaaacg cagagtttgt tgaaggttct gacaaactgc
     3601 tgcgcctgac actggatctc ggcggtgaaa aacgcaatgt cttctccggt attcgttctg
     3661 cctacccgga tccgcaggca ctgatcgggc gtcacaccat tatggtggcc aacctggcac
     3721 cgcgtaaaat gcgctttggt atctccgaag gcatggtaat ggctgctggt cctggcggga
     3781 aagatatttt cctgctaagc ccggatgccg gtgctaaacc gggtcatcag gtgaaataat
     3841 cccccttcaa ggcgctgcat cgacagcgcc ttttctttat aaattcctaa agttgttttc
     3901 ttgcgatttt gtctctctct aacccgcata aatactggta gcatcagcat tcaactggat
     3961 aaaattacag ggatgcagaa tgaaacactt tatctatcag gacgaaaaat cacataaatt
     4021 ctgggcagtt gagcaacagg gaaacgagtt gcatatcagt tggggaaaag ttggcaccaa
     4081 agggcaaagc cagataaaaa gtttttcaga tgctgcggca gcggcaaaag cggagcttaa
     4141 gctgattgcg gagaaggtga agaaggggta tgtggagcaa gcgaaggata attctttgca
     4201 accttcccaa acggtaacgg gctctctcaa ggtagcggac ttatccacca ttattcagga
     4261 acaaccctct ttcgtagcag aaacccgtgc gcctgacaaa aatacagatg ctgttttacc
     4321 gtggctggcg aaagatattg ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag
     4381 tcatcgccgc tttcccggag ttcctgttca gcaagcagac aaattgaccc aattacgtcg
     4441 cttagcctgt agtgtgtcgc aacgggataa taaaacagcc acatttgact tcagcgcctg
     4501 ttctttagaa tggcaaaaca ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac
     4561 aacacagcta ccatcaccag taatggctgt actcacggca cttgaaatga aatgcacaag
     4621 atataaagtg cgtgaggatg ttatggatca gatcgtccag gaaggcggtc tggaatatgc
     4681 tactgatgta ataatacacc ttcaacagat tgatattaaa tgggattatg cgaataatgt
     4741 cattattatt ctgccgtctg gcattgcacc tgactacttg gagcaatatt ccagatttga
     4801 attacgccta cgtaaacatt tatcactggc ggaagagtct ctctggcaaa aatgtgcaca
     4861 aaaacttatt gccgcaattc cacatattcc agaatgacgg caaccattaa ttgctttgtt
     4921 attacctgaa aaaccagaaa ttgcacatga aattgcccag cgtctactgg ggcaaaaaaa
     4981 attaccctcg cttgagtggt taaaaatagt ggcgactgat gagcacattc ttgcctcatt
     5041 agaaaaatat cacgagccat atgccatttt tgatgattac tattgtggtg cgatatggtc
     5101 agccaccgta ttacaggagc aaggtgttgc agccctgccc cgatttgctc cctatgccgc
     5161 aagtgactac tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat taacactgct
     5221 tatacgtgta gccgggcata ctaaacgctg tcacgatcgg atgacgaaag ccattgctgc
     5281 gttcccacat gcagcaatgg cggcactgac ggaacttctt gggcaaaaag aagagaacag
     5341 ttggcgcatt atgctaatga caatgcttat ctcacaacca gcactggcag aacaggtcat
     5401 tccctggctc tcgacacccg cagttgccgt actgaaatca tgccagcaac aactgacaca
     5461 gccctcaaac catgccagcg ccgatctact gccagccgta gtagtctccc ctccctggct
     5521 ttcgaaaaag aaaaaatcgc cgattccggt gctggattta gcgccattag gcattgagcc
     5581 aatctgttat ctgacagaag aaatcagtaa tcaacttttg gcgaaatata tctggtattc
     5641 aaaacacatc acggttagcc atgaagaaag tactaccaac ctgttggcaa ggatgggttt
     5701 tcaacgacgg atcgctggta catatattaa agctcccgaa gcggtagttg aggcatggct
     5761 aaatgaagat tattcaacct tactaagtga atttaaggtg tttcattcac ctaccgggca
     5821 ttattggcag ttggggattt tgacaacatt gccgctggag aaagcagtaa aagcatggaa
     5881 tgcccttacc ctatctccac ataccgatac cgaatacgcc atgttacatt ttggactcaa
     5941 agggttacct gggttagtaa actcacttgc acgctatcca caagaagcct tgcccatcac
     6001 gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca acaaactgaa
     6061 aacgctacgc gaaaacgccc gtagctggct gttgaaatac ccggaacatg cccttaccgg
     6121 cctgctgcct gcggcgctcg gcaaagccgg tgaagcacag gataacgccc gcgctgcctt
     6181 gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc gacgttataa
     6241 ccagccggaa gtaaccgatg cggtgaacgc tctgcttgcg ctcgatccct tagataatca
     6301 cccgacaaaa atccccactc ttccggcctt ttatcagcca tcgctctgga cgcgcccggt
     6361 attaaaagca aatgcccaat cactgccaga tagcgccctc ctccacctcg gtgaaatgct
     6421 ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag acgtctgttc
     6481 cgccgactca ctggcgggat ttgcctggga tctgtttacc gcctggcaga ccgctggcgc
     6541 gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg atgacaccgc
     6601 ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata aacgcgccac
     6661 cgttgggttg gatattctcg ctgctatcgg tagtgatatc gcccttatgc agcttaacgg
     6721 catcgcccag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa aaattgccga
     6781 cattgccgag agccgcgaac tcacggtggc ggagcttgaa gatcggttag caccggatct
     6841 cggtctggat gataacggtt cgctgctgct ggattttggc ccacggcagt tcaccgtcag
     6901 ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc tgaaagacct
     6961 gcccaaaccg aacaaaagcg atgatgaatc gcggtcgaac gatgcggtta accgctacaa
     7021 attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc tggaatccgc
     7081 catgtgcctg cgccgccgct ggtcgccgga aaacttccag ctcttcctgg ttgagcatcc
     7141 gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg aaaaccagct
     7201 actggcttgc tttcgcgtag cagaagataa cagctacagc accgctgacg atgatctttt
     7261 caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaga tatcaccgac
     7321 ggatgccgcc gcctttgggc agctttttgc cgactatgaa ctgttaccgc cgttccgcca
     7381 gctcgaccgt aacagttacg ccctgacaga aaccgagcgc aatgccagcg aattgacccg
     7441 ctgggcaggc agaaaatgcc cgagcggtcg ggtcatggga ctggcgaata aaggttgggt
     7501 gcgtggtgaa ccgcaggatg gtggctggat cggctggatg atcaaacctc tgggccgctg
     7561 gtcgttaatc atggaaatcg atgaaggctt tgcagttggt atgtcgccag ccgaacttag
     7621 cgccgagcaa ctcttaagca agctgtggct atgggaaggc aaagcagaaa gctatggctg
     7681 ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca ccgccagcga
     7741 gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca aggaattacc
     7801 gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa cgccgctcag
     7861 tcatcgtcgc tggccgggcg aaccagtgtc cgttatcact ggaagtctca tccagacatt
     7921 gggagatgaa ttactacaac aagcaggcca gaaggaaaat atcacctgga attatgataa
     7981 atgctcactg gagtggcagt ccgccatcca gcaggcaatc aatttgactg gcgagcataa
     8041 accgtcaatc ccagccctta ccatggcggc tctgatctgt atcgcgcaaa atgacagcca
     8101 gcagttactt gatgaaatcg tgcaacaaga ggggctggaa tatgcgacgg atgtagtgat
     8161 agcgcgtcag tgcattgcgc gacgttatga gagtgactca ctggtcgtca ctttgcagta
     8221 tcaggacgag gactatggct acggttatgg ctccgcgacc tataacgatt tcgatctccg
     8281 tttgcgtaag catctgtcgc tggcagagga aagttgctgg cagcgttgtg ccgataaact
     8341 cattgccgca ctgccaggaa tacccaaaat tcgccgtcct tttattgcgc tgatcctccc
     8401 ggaaaaacct gaaatagcca atgaactcgc cagtcttgaa agctctcggt caagtctcca
     8461 ttcaaaggag tggttaaagg tcgttgctac tgataatacg gcggtaaaaa aactcgaacg
     8521 atactggggc ctggacgtat ttagcgatcg cgaagccagc tacatgtcac aagaaaaccg
     8581 cttcggctac gcggcctgcg cctccctttt gcgcgaacaa ggactggcag ccgttccgcg
     8641 cctggcaatg tatgcccata aagaagattg cggcagtctg ctggtacaaa ttaaccatcc
     8701 gcaagtcatc cgcaccttgc tactggtggc tgataaaaac aaacccagcc tgcaacgtgt
     8761 ggctaaatac agtaaaaact tcccccacgc gacgctcgcc gcactggcag aactgctggc
     8821 gttaaaagaa ccaccagccc gcccaggtta tccaatcatc gaagacaaaa agctgcctgc
     8881 acagcaaaaa gcacgcgatg aatactggcg tacgctgtta caaacgctga tggcatcgca
     8941 gccacaactg gcagcagaag tgatgccgtg gttaagtact caagccaggg cagtgctgaa
     9001 tagttattta tcggcaccgc caaaaccggt tattgatagc accgataaca gcagtctgcc
     9061 tgaaatgctc gtttcaccac cgtggcgtag taagaaaaaa atgacagctc cacgtcttga
     9121 tttggcaccg ctcgaattaa ctccgcaaat ttactggcaa ccaggcgaac aagagaggct
     9181 tgccgccact gagtctgccc gttatttcag cacggaatct cttgcggaac gcatggaaca
     9241 aaaaagtgga cgagttgtat tacaggaact gggttttggg gatgatgtat ggctgtttct
     9301 gaattatata ctccccggaa aactggatgc tgcacgcaat tcactcattg ttcagtggca
     9361 ttactaccag gggcgggttg aagagatcct gaatggctgg aactccccgc aagcacaatt
     9421 agcagaacag gcgctccgca gcggtcacat agaagcgtta attaacatat gggaaaatga
     9481 caacttctca cgttatcgtc cggaaaagag tgtctggaac ctgtatttat tggcacagtt
     9541 gccgcgtgag atggctttga ccttctggct gcgtatcatt gagaaaaagc atctgttcgc
     9601 gggtgaggac tattttctca gtatcctcgg attggatgcg ctaccaggtc tgctgttggc
     9661 tttttcacat cgtccaaaag aaacatttcc gttaatttta aattttggcg caacagaact
     9721 ggcgctgcct gttgcccgcg tctggcgacg tttcgcggcg cagcgtgatc tggctcgcca
     9781 gtggatttta caatggccgg aacatacggc tagtgcactt atccctcttg tctttaccaa
     9841 acccagcgat aatagcgaag ccgcattact tgccctgcgt ttactgtacg aacagggaca
     9901 tggcgaattg ctacaaaccg tggcaaaccg ctggcagcgt acagatgtat ggtctgccct
     9961 ggagcagttg cttaaacagg gtccaatgga catttacccg gcacgcattc caaaagcccc
    10021 tgatttctgg catccggcaa tgtggtccag gcctcgactt atcactaata atcagcctgt
    10081 taccggtgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg ggggacgttt
    10141 ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg cagcttttgc
    10201 ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc gcaaaagaca actgggcatt
    10261 tctggcgtta agtctctttg gtgacgaaag cacggcacgg gatctgacga cacagatcct
    10321 cgcctggcca caagaaggca aatctgcccg tgctgtcagc ggcctgaaca tccttaccct
    10381 gatgaataat gatatggcgc tgatacagct gcatcatata tcgcaacggg ctaaatcccg
    10441 ccccttacgt gataacgcgg cggaatttct tcaggtagtc gcagaaaatc gcgggctaag
    10501 ccaggaagag ctagcggaca gattagtccc aaccctgggc cttgatgatc cgcaggcgtt
    10561 gagttttgat tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc tcaacccggt
    10621 tatctttaat cagcaaaacg ttcgccagaa aagcgttccc cggttgcgcg ccgatgacga
    10681 tcaactgaaa gcgcccgagg cactggcccg actaaaaggg ctaaaaaaag atgctactca
    10741 ggtgagcaaa aacctgctcc cgcgtcttga agctgcccta cgtaccaccc gacgctggtc
    10801 gctggcagat tttcattctc tgtttgttaa tcatcccttt acccgtctgg ttacccagcg
    10861 attaatatgg ggggtttatc cggcaaatga accgcgttgt ttactcaaag cctttcgtgt
    10921 ggccgcagag ggggagttct gcaatgcgca agatgagcca attgacctgc ctgcggacgc
    10981 tctgattggc attgcccacc cgttagaaat gacagtagaa atgcgcagtg aatttgcaca
    11041 gctttttgcc gattacgaaa ttatgccgcc ttttcgccag ttgtcgcgcc gcacggtgct
    11101 gctcacacct gacgagtcaa ccagtaacag cctgactcgc tgggaaggta aatccgctac
    11161 cgttgggcag cttatgggaa tgcgatacaa aggctgggag tcaggttatg aggacgcatt
    11221 tgtctatgac ctgggtgagt accggctggt ccttaagttt tcacccggtt ttaaccacta
    11281 caatgttgat agcaaagcgc taatgagctt ccgttctctt cgagtgcacc gtgacaataa
    11341 atccgtcact tttgccgaac ttgatgtgtt tgatttgagt gaggcgttaa gcgcacctga
    11401 cgtcattttc cattaaaaac acagcgggca gtgatgcaac tgcccgttat caacacgact
    11461 taccagcggg gaaagcgatg atcgtgcaaa aagagctggt tgctatttac gattatgaga
    11521 tccctgtacc tgaaaatccg ttttccttca gacttgagat ccataaatgc tctgaattat
    11581 ttacaggttc cgtctatcga ctggagcgat tccggctacg tccaacattt catcaacgtg
    11641 atcgagaaga tactgacccg ctaataaatg atgcgttgat ttatataaga gatgagtgta
    11701 ttgatgagcg gaaattacga ggtgaatcac ctgaaactgt aatagcaatt tttaatcgtg
    11761 aactacagaa tatattcaac caagaaatag agtaatatac tctagataat taaagtcgca
    11821 gcacatgcaa cttgaagtat gacgagtata gctttcttaa agggggaaac ggtattcccc
    11881 cttagatgcg tttcaatttg cccgttactt caacaaatca atcaaccgta acagagccac
    11941 taataactcc agtacggcaa tgagcacggt taacttttgt gtcagcgtgt ccatccgctc
    12001 tccttattaa ggagcgcaac accagcacct tacccttaca ctgcctgttg atactggttt
    12061 acgtatgttg gtacgaaacg ttgctctccg gatcagggca ccgcctgcgg caccacccgt
    12121 ccggcagcca ggacttaaaa ttggtccgat ccggcacaac ctcccaacgg gcaggattat
    12181 aaatctcact cactgaattg caatggagtg tggatatggt tataatttat tcgttggtac
    12241 gaaatgttgc acaatcagca agttagcgtc actgtagtaa ataaaagaac ctgcctcacc
    12301 agcaggtttt tttattttct gcgatctact ttccagatat tttttgccaa tagcccaaca
    12361 taaatattca catcccggtc aaatggtgcg ttgaatataa aatcgatgaa tttataaata
    12421 aaaatgagtt atatgatgaa ttaataaaaa cagtaatcat tttattaaag ggggaagcgg
    12481 t
//
LOCUS       DAZFDP010000136        12464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFDP010000136 DAZFDP010000000
VERSION     DAZFDP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            224..1054
                     /gene="yjiC"
                     /locus_tag="ACHJ1G_003903"
     CDS             224..1054
                     /gene="yjiC"
                     /locus_tag="ACHJ1G_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4765672.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1195..1968)
                     /gene="uxuR"
                     /locus_tag="ACHJ1G_003904"
     CDS             complement(1195..1968)
                     /gene="uxuR"
                     /locus_tag="ACHJ1G_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4765673.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2183..3643)
                     /gene="uxuB"
                     /locus_tag="ACHJ1G_003905"
     CDS             complement(2183..3643)
                     /gene="uxuB"
                     /locus_tag="ACHJ1G_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4765674.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3724..4908)
                     /gene="uxuA"
                     /locus_tag="ACHJ1G_003906"
     CDS             complement(3724..4908)
                     /gene="uxuA"
                     /locus_tag="ACHJ1G_003906"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4765675.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5360..5452
                     /locus_tag="ACHJ1G_003907"
                     /pseudo
     CDS             5360..5452
                     /locus_tag="ACHJ1G_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5695..6597)
                     /gene="fimH"
                     /locus_tag="ACHJ1G_003908"
     CDS             complement(5695..6597)
                     /gene="fimH"
                     /locus_tag="ACHJ1G_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4765676.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6617..7120)
                     /gene="fimG"
                     /locus_tag="ACHJ1G_003909"
     CDS             complement(6617..7120)
                     /gene="fimG"
                     /locus_tag="ACHJ1G_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO4765677.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7133..7663)
                     /gene="fimF"
                     /locus_tag="ACHJ1G_003910"
     CDS             complement(7133..7663)
                     /gene="fimF"
                     /locus_tag="ACHJ1G_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4765678.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7673..10309)
                     /gene="fimD"
                     /locus_tag="ACHJ1G_003911"
     CDS             complement(7673..10309)
                     /gene="fimD"
                     /locus_tag="ACHJ1G_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4765679.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10376..11101)
                     /gene="fimC"
                     /locus_tag="ACHJ1G_003912"
     CDS             complement(10376..11101)
                     /gene="fimC"
                     /locus_tag="ACHJ1G_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4765680.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11138..11677)
                     /gene="fimI"
                     /locus_tag="ACHJ1G_003913"
     CDS             complement(11138..11677)
                     /gene="fimI"
                     /locus_tag="ACHJ1G_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4765681.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11742..12290)
                     /gene="fimA"
                     /locus_tag="ACHJ1G_003914"
     CDS             complement(11742..12290)
                     /gene="fimA"
                     /locus_tag="ACHJ1G_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4765682.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3105 a         3234 c         2939 g         3186 t
ORIGIN      
        1 aaattccaat tagtaataag atatgctttt aaattgcatg aaaataattt caaatgagcg
       61 ttttttgata ctttagagtg tttcctaccc tttaatatca ccgtgtagag ttgatatcac
      121 tacaacagaa acatgcatgt catttgttta cttctcattt tactacagaa aaatttatct
      181 atttttctaa acatcagtga aatactcata aaaggtaact attatgtcta tgccattaag
      241 caataccctt cagagccaaa ttattacaga taatcacttc cttcatcatc caaaagtcga
      301 cagtgagtta acacgtaaat atgaacgtgc acgcctggat acggaaaata tctatcttct
      361 gcctcttgct cgtggcaata accataatta cgatggaaaa agcgttgtcg aaattagaaa
      421 acttgatatt agtaaagagt cctggccatt taattatgtg acagaagcat gcagagaatc
      481 cgatggtatt acaacaacag gacgtatgct ctatcgaaac ttgaaaatca cctcagcttt
      541 agacgaaata tatggtggca tatgtaaaaa ggcgcatgct gctacagaat tagcagaagg
      601 tttacggcta aatttattta tgaagagccc tttcgatcct gttgagaact atacagttca
      661 tgaaataaca ttaggccctg gttgcaatgt tccaggttac gccggaacca cgataggata
      721 tatttcaacc cttccagttt ctcaggcaaa aagatggacc aatgagcagc caagaattga
      781 tatctatatt gatcagataa taactgtctc aggggtagca aattcttccg gattcgccct
      841 tgccgcatta cttaatgcta atattgagat gggaaatgac ccaatcattg gtatagaagc
      901 ctatcctggt accgcggaga tccatgcaaa aatgggatat aaagttatcc ccggtgatga
      961 agatgcgcca ctcaaacgca tgactctgca accgtcatct cttccagaac tgtttgaatt
     1021 aaaaaatggt gaatggaatt atattggtaa atagccactg agaagaatga gtactaatat
     1081 tcaggttata cagatattta aacaataccc tgcaatttac aaagcaatac tcattgcgac
     1141 ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg cttattataa taagtcagac
     1201 gtcgacttta tccagcggcc atgaatcaaa cagatagccg tcaaaataaa tatcgtcaac
     1261 gttcgagaat tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt
     1321 cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca
     1381 ctctttgcga tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt
     1441 ttcccgccac tgccaggact gacggaataa ctccaccagc atgctgttat gcgttgcttc
     1501 ggcaatagcg agatggaact gcatgtcacc gctttcgctg ctgcccggcg cactggaagc
     1561 cagttcacgc tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc
     1621 ctgcaaagcg gcaaactcgg cgatgttgct ctccaataac tgccgcgcct gtaacagctc
     1681 aaaaggaccg gcatcgttgc agacgttggc atccggactg tctgtgttct ggctgcctga
     1741 gttatcaaga acatagatac cggcaccccg gcgtacttcc accagccctt tgatctccag
     1801 catgatcagc gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg
     1861 ctccggcggc agccgttcgc caggattgta cggcgtcttt atgatcagat cgcggatcat
     1921 cgccccgact tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc
     1981 gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca
     2041 acgggcaatt ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca
     2101 cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta
     2161 agtctgtagg gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc
     2221 acagctgctg atatgccgcc gtcactgcgc caacaaagtc ggcattctgc ggcagatcat
     2281 cggcaaaaat accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg
     2341 cgttgacctt ctggaactcc gccagcatcg ggtcgaccac gtcaatggca ttaccctgct
     2401 catccacgcc ctgggtgtaa cgcatccagc cagccacgcc cagcgccagg tgacgccagc
     2461 tgccgccgtt ttgcaggtgc agacgcaccg ggtccagcag acgctgcggt aacttctggc
     2521 tgccgtccat cgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga
     2581 tcagcagcgt cgcataggcg ttcaggtccg taccttccgg catcgacagc gttggcgctt
     2641 gttcctgcat catcaaggag aaggccgctt tgcgataatc cgggttagtc atggtgtcgg
     2701 caatggtttc atagccgccg aggtaaccga ggtatgccag gaaagagtgg ctgccgttca
     2761 gcatacgcag cttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt
     2821 tatcccagtc cgggcgaccg ttaacgaagt tatcttcaat cacccactga cggaacggtt
     2881 cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgcaaggttt
     2941 ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttttctt
     3001 caatccatgc cgccagctgc gggtcacgcg cctgagccag tcccagtacc gcgaccttcg
     3061 ccacatggcc gttttcacgc acgttatcgc aggacatcac cgtaaacgct ttcaaccctt
     3121 tttcacgacg cagccgcagg gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg
     3181 ggttttccag atcgtgcttg atcagtgggt tattgagatc cagctgaccg cttgccgcat
     3241 cagcgcagta gcctttttcc gtgactgtca gagagacaat cgccgtctgc ggacgcgcca
     3301 tcgcattgag aataccttcg cagccgtcga tttccggatg cagcgcttct ttcatcgaac
     3361 cgataatttt cagctcggtg ctttccgcgc ctttttccgc cacggtgtac agcagttgct
     3421 gttttttcag gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga
     3481 taccccagtc gctgtcggtg ctttccagca ggtgatgggt atacagcgcc tggtgcgcgc
     3541 ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgctcccatg
     3601 acgggcgggc aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc
     3661 gtcttcagag agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg
     3721 gtattaacgg ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag
     3781 gcctttcaga cgaccaattg cggagtaacc tgggttggtt ttcttcttca ggtcgtccag
     3841 catctgatga ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg
     3901 acggtgttct tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag
     3961 gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt
     4021 gaagtaaata cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac
     4081 gccgtaggaa ccggtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat
     4141 ccactgcata tcttcaatgg tggaaacaat gcgcggcagg ccaaggatcg ggcgcggcgg
     4201 atcgtccggg tgaaccgcca tacgcacgcc aacttcttca gcaactggaa taatcgcttt
     4261 caggaagacg gcaaagtttt cgcgcagctt cgctttatcg atatctttgt acagctccag
     4321 gtgcttacgg aactggtcga gggtataccc ttcttccgcg cccggcagac cggcaatgat
     4381 gttacgcgtc agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgaa cagcagcctg
     4441 agcaatttct tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc
     4501 gaatgcagcg aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta
     4561 ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat
     4621 gccgcactgc gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt
     4681 gccagtgtgg gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag
     4741 gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc
     4801 gttcgggata tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc
     4861 taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct
     4921 ttcacatagt gggttccaca gcaaagagac gcggaaccct ggagttgttt aggtatccgg
     4981 atgaacaacc agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc
     5041 ttttggtaag acatgtcaac ccgaattctg aaattggtta accacatcac aagaatttca
     5101 ctctcatggt tgttgctgca aattgcagca aagggtaatc gatggttatt gatcagagga
     5161 tgtgacattc atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa
     5221 cggttcaacc aggtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc
     5281 ggcattggcc tgatgctggt actgacaaag accgcaggtt cagacacatt cgctgcatta
     5341 gcaatgcctc ttagataatt ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     5401 gctggagctg gtcaactccg tggttgggct gattattagc aaggtagcgt aaaagaacac
     5461 cagagcctgc caatggcagg ctctgactga tgacaaagtc taaaaatgtg cccggacagt
     5521 cccctcgccc ctccggggag agggttaggg tgaggggaac agaccagcaa agtgcgcttt
     5581 gtttatgcca gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg
     5641 tttttagctt caggtaatat tgcgtaactg cattagcaat gccctgtgat ttctttattg
     5701 ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt
     5761 acgtgcgtaa tttgccgtta atcccagact taccgccgaa gtccctactg ctcctaacga
     5821 taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg
     5881 cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatcta cggttgtgcc
     5941 ggagaggtaa taccccagat tttggctttt cgcacaataa acggtaagag gaattggcac
     6001 tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat cgcagccgcc
     6061 agtaggcacc accacatcat tattggcgta aatattccac acaaactgaa aatcatcgct
     6121 gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag ctttaatcgc
     6181 caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt
     6241 tctcgaatta taaacaacgc gcggcgtttc gctggtggta ggaaatggat cgctactgcc
     6301 actatatttt acggtcccgg aaaaattaga taacacgctg ccataagccg agcctcgttg
     6361 cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga
     6421 aagatccacg accaggtttt gccccacatt cacggcgggc gcaaggttta cataaacatt
     6481 ggcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg cgaatgacca
     6541 ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc gtttcattac
     6601 aatcatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc
     6661 cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag tgcgctgatt
     6721 gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt
     6781 catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtac
     6841 tgtcggctgc cccgctgaag ctggcggtga ccctcgacgt tcccaccgga caattagtca
     6901 actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga ctgaaagaat
     6961 aaagatcgcc gagatcaacc gtggcattgg tggtggaaac cgtacacggt ttggcgacga
     7021 ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca
     7081 atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc agttactgat
     7141 attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg
     7201 ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg
     7261 aaatcgcgga cgatggagca ttaaggggta tttgattttg ctgctcattc agaagctgta
     7321 ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcagattg gcattgtggc
     7381 tatctgcaac gccggtaaac ccaactttta cggcagaaac ggcattacca cagggtgaca
     7441 gcaaaatacg aaatggaaca acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg
     7501 cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat
     7561 ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaagcgtga ctcaccgcca
     7621 gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac
     7681 attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg
     7741 cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca
     7801 ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg
     7861 atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc atgagcagtt
     7921 ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt
     7981 tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc agcgccactc
     8041 tgttttccct atattcagtg gcataaggca gcacggcata accgcgccag tcggtacgca
     8101 cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg
     8161 tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccaatacc ccaccgctga
     8221 ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg gcattgccgt
     8281 aaccaccgcg ataattcagc gtggcgtagc ctgtgcttcc gctattacca tcgcctcccc
     8341 cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac gtaccgtata
     8401 caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag ctggcactgg
     8461 catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga atattgacgt
     8521 taagcgctaa catctgatca cgtccttttt gccaggcgtt tttcgtcagg ctatagctga
     8581 gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc tcatcgacat
     8641 tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat gtgcgcccga
     8701 gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt
     8761 cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg tagccattca
     8821 ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa cggtaaccca
     8881 ctaactgaat attcgtgcct gattcattga gcgatttgtt atagagaaaa cgcaccgatt
     8941 gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca
     9001 gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga taacgatccg
     9061 ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct
     9121 ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct gccgtaatgg
     9181 aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa
     9241 tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta ttacctgcgg
     9301 catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa atgtcatacc
     9361 cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa
     9421 atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt
     9481 taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc cgggaacgta
     9541 acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt ttgctacctg
     9601 atgatctgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc cacgcaccaa
     9661 tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca atccgattct
     9721 gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg ggatcccata
     9781 actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt
     9841 tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc ctggaccatt gtggttaatg
     9901 gcacacaggc atcatccgcc agcagattca taccggcgac agaagccgta ttcagcccca
     9961 tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg tcgcccgtat
    10021 taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg acgcgatacg
    10081 tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca gcctggggat
    10141 catccgctaa aaaacgcggg ttaaaataga gttcggcaga tgacaaaggt gcctgtgcgg
    10201 caaaagcaca ggcaacaaag agccggacaa aaaaaccagc caaacgatgc ttacgaatat
    10261 gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg
    10321 tttaaagtca cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt
    10381 ccattacgcc cgtcattttg ggggtaagtg caccataatc atttattgtt cggtaagtaa
    10441 tattgcttcc cgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaagg
    10501 cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg
    10561 ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat
    10621 cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg ataattgcga
    10681 gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct ttaacattca
    10741 tccagaataa actttcccga tcctgtggca attggttatt tgttgcatca agaatacgca
    10801 aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata aaacgaccat
    10861 cctttacacc atcggcattt tccacccatg attgaattaa ataggtacta ttttcatcat
    10921 tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg cgagtcgcac
    10981 ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccataaac atcaggatac
    11041 ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca
    11101 ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt aaagagaacc
    11161 aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga
    11221 tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga ggacgattaa
    11281 tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg gctatccctg
    11341 gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca
    11401 cccccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg
    11461 gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct gatttgcccc atattgaccg
    11521 tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg ccctgaaatt
    11581 gcatatttcc gccgggcagc gtggtattcc atttatttcc ggccagagca aacattggca
    11641 gcaacgacgc taacagaaat aatctcttcc ttttcatcgt tatttttatc gcacaagggt
    11701 gggcatccct gcccgtaatg acgtccttga acctgggtag gttattgata ctgaaccttg
    11761 aaggttgcat ccgcattagc tgcacccggg gttgcctcgc cgattgcata ataacgcgcc
    11821 tggaacggaa tggtgttggt accgttattc agggttgttt gtgcactaaa tgtcgcaccg
    11881 tccagcgtca gagcattgcc tgttctgtcc aggatctgca cgccaacgtt tgttgcacta
    11941 cctgcagcag aactctgcag agccagtaca tcagtacgcg tagcatcaat tgccgtacct
    12001 aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc aatcattcaa ctgaatgtta
    12061 aaaccaacgg cagagctggt tgctccagcc tgcttcaggc tagcggtacg aacctgtccc
    12121 aactgaacgg tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacttcc
    12181 cctttaaagt gaacggtccc accatttacc gtcgtagtat cggccagagc cgctgcggaa
    12241 ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat gctgctttcc
    12301 tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa
    12361 cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt tggggccatt
    12421 ttgactcata gaggaaagca tcgcgcacaa actttttcag ttta
//
LOCUS       DAZFDP010000137        12327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFDP010000137 DAZFDP010000000
VERSION     DAZFDP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="ACHJ1G_003915"
     CDS             complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="ACHJ1G_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4765683.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVL"
     gene            506..1207
                     /locus_tag="ACHJ1G_003916"
     CDS             506..1207
                     /locus_tag="ACHJ1G_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4765684.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1230..1394
                     /gene="yhaL"
                     /locus_tag="ACHJ1G_003917"
     CDS             1230..1394
                     /gene="yhaL"
                     /locus_tag="ACHJ1G_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4765685.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1606..2916)
                     /gene="cyuA"
                     /locus_tag="ACHJ1G_003918"
     CDS             complement(1606..2916)
                     /gene="cyuA"
                     /locus_tag="ACHJ1G_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4765686.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2944..4275)
                     /locus_tag="ACHJ1G_003919"
     CDS             complement(2944..4275)
                     /locus_tag="ACHJ1G_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4765687.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4550..5914)
                     /gene="tdcG"
                     /locus_tag="ACHJ1G_003920"
     CDS             complement(4550..5914)
                     /gene="tdcG"
                     /locus_tag="ACHJ1G_003920"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4765688.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5986..6375)
                     /locus_tag="ACHJ1G_003921"
     CDS             complement(5986..6375)
                     /locus_tag="ACHJ1G_003921"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4765689.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6389..8683)
                     /gene="tdcE"
                     /locus_tag="ACHJ1G_003922"
     CDS             complement(6389..8683)
                     /gene="tdcE"
                     /locus_tag="ACHJ1G_003922"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4765690.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8717..9925)
                     /gene="tdcD"
                     /locus_tag="ACHJ1G_003923"
     CDS             complement(8717..9925)
                     /gene="tdcD"
                     /locus_tag="ACHJ1G_003923"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4765691.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9951..11282)
                     /gene="tdcC"
                     /locus_tag="ACHJ1G_003924"
     CDS             complement(9951..11282)
                     /gene="tdcC"
                     /locus_tag="ACHJ1G_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4765692.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11304..12293)
                     /gene="tdcB"
                     /locus_tag="ACHJ1G_003925"
     CDS             complement(11304..12293)
                     /gene="tdcB"
                     /locus_tag="ACHJ1G_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4765693.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3160 a         3362 c         2953 g         2852 t
ORIGIN      
        1 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
       61 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
      121 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
      181 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
      241 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcagaaggc
      301 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
      361 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
      421 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
      481 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
      541 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
      601 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccagatgc
      661 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
      721 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
      781 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
      841 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
      901 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta cgatgggaag
      961 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     1021 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     1081 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     1141 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     1201 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     1261 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     1321 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     1381 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     1441 atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc cagcatcgca
     1501 tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt aggccggata
     1561 aggtgcgcca gcatcgcatc cggcacgata cccaaaacct ggcgtttatc tggccttgct
     1621 cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca
     1681 caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc cggtcacggc
     1741 ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt
     1801 catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc cgatcatact
     1861 gctgatcgcc atcgagatgg tttcataacg cccatccacc agccatgcca tcccggcggc
     1921 ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg
     1981 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagccgtt catcatccgc
     2041 tccgaagtgt tctgctacca ccaccacagg cattgttgcg gtgatcccct ggttacccga
     2101 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
     2161 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
     2221 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
     2281 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac
     2341 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggag actcttgctc
     2401 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
     2461 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
     2521 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacggaga ctttccccgc
     2581 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
     2641 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
     2701 tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg aaacccaggc
     2761 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
     2821 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
     2881 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
     2941 aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag
     3001 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
     3061 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
     3121 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
     3181 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
     3241 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
     3301 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
     3361 tgcggcaatc gccagcgcgg aaatattctg ctcataggct tttaccgctt cgtcatgtcc
     3421 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
     3481 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
     3541 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
     3601 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
     3661 cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca gcttggtcag
     3721 caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
     3781 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
     3841 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
     3901 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
     3961 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
     4021 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
     4081 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
     4141 gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
     4201 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
     4261 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
     4321 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
     4381 atacgcgcga ggtcagagaa aatcttcaca atcaagactt tgaaataacg gagatggata
     4441 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
     4501 aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt cagccgcaga
     4561 ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc atatctttgc
     4621 cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc gaggtgcggc
     4681 gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta cgttcaatgc
     4741 acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta tgctccatcg
     4801 cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca gttaaccctg
     4861 ccgccgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc gcgccagaga
     4921 tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa
     4981 ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact gccggaataa
     5041 tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac
     5101 taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg ttagagatgt
     5161 tatcgctgga aaccagctga cgacgcagcg ctacggcacg acgcggcaca ttgagcggac
     5221 caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc acttgccaga
     5281 tacgggcaaa ccctgcgtca atttccgctt tgctgcgcag cgccagctcg ttgtgcatca
     5341 tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca cctgctgagt
     5401 ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga
     5461 caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc
     5521 aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg aagataatgt
     5581 tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc cgcccgctgc
     5641 gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc ggactgtttc
     5701 ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc agtgacaacg
     5761 acccatacag atcgaccaca atatggctcg tcgcggttaa taagccgcta ctttccagcc
     5821 gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg gagggaccaa
     5881 tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat tgccgttcag
     5941 tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg cactacgtac
     6001 tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct gcacacagct
     6061 ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt cattgatggt
     6121 ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc ccacgctcag
     6181 cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac
     6241 atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg tgaagaccat
     6301 gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac gttgcgtttc
     6361 gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa
     6421 catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga
     6481 ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt tcccgattca
     6541 ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat
     6601 ccagcaggcc gacaaggttg gttttacgta ctggatcttc tttgcccagc gccgcaggaa
     6661 cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt ttcgccaccg
     6721 acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta gcgcccggcg
     6781 cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg ccgtacacca
     6841 cgttagaagt gatagtcaga atcgactggg tagtgacggc gttgcgatag gttggcagcg
     6901 ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt
     6961 cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt
     7021 cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc gtcgccaccg
     7081 acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc agcgccatca
     7141 gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt
     7201 actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg tcgtaatcca
     7261 gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc ttctcgtcca
     7321 ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact
     7381 gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt
     7441 tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg acgatcgaca
     7501 cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt
     7561 taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag gagtttttgg
     7621 tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc cagattgggt
     7681 cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata cggatcttca
     7741 tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gagtacgcca gctttaaagt
     7801 cgcgctcaat gtagatatcg aggaacgatg ccgtgcggcc cagcgacatc gcgccgccat
     7861 tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct tcctgcgcat
     7921 tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga atctgcaaca
     7981 gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc agatcctcgc
     8041 ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgt accagataac
     8101 tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacgg ccatagccat
     8161 ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt gagtaaacat
     8221 caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca aattcactgt
     8281 ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca ccgaacgggt
     8341 gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc agcggctggt
     8401 taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg aaatcaaccg
     8461 gcgcgtgggt tgcgttttcg atacggatac cttccattac tttttcccac aattccgtgg
     8521 tggcaggcgt cgcttcggcg aggaaagatt catcgccttc atacggtgta tagttatgtt
     8581 gaataaaatc gcggacatta atttcgtttt tccagtccgt acctttaaag ccaagccatg
     8641 cgtcggcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat aatctctcta
     8701 caatacttca actaaattat gcaaattctg cgggcgcgtt aactttgcct aaatgaatgg
     8761 catccaaagc aatcattttt tcttcgttag tcggaataac agcgcaaatg acacgcgcat
     8821 tttcactgga aacaattcgc tcaccacagg agttagagcg attattcatt tctgtatcaa
     8881 tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaag cttgaattct
     8941 ctcctattcc gccggtgaat ataattccat ccaggcgacg taatgaagct gcgtgtccgg
     9001 caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac
     9061 cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa atacctaata
     9121 atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg gtttggctgg
     9181 cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc atcaagcctt
     9241 ccagcggcgt cattcccatt gaagtatcaa cactctgacc gttgcgaacc gcgcagattg
     9301 acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc agattcagca
     9361 gcgaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca taacggcgta
     9421 cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc ggagccatcg
     9481 tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat aattgctgcg
     9541 ccgattcaat accacttaaa ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac
     9601 ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata ctgccgccgt
     9661 gagcgatgcg gtggccaatt aaggccacac tgtcatttaa attccgtttt tccagttcaa
     9721 atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc
     9781 catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct gacattaata
     9841 cttcacagtc gctggcatcg agtacggaaa acttaatcga agacgaacca cagttaataa
     9901 ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga ttaaaacagt
     9961 ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac gttatccaga
    10021 cgaccacggt atttcgccag agacggcgct ttacggatgg catacatcgg caacaggcac
    10081 agcagggatg cgataatcgg tgcgcccatg gcttcaataa ggtcgaggat gttcgggttg
    10141 gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgatagt gttcagttta
    10201 cccagcgaca ctttagtttt gtcgccttta taaccgaact tcagaatcag accattcaag
    10261 ccttccagcg tccccagata gtgaccgaag aaagatttga agatagccac gagtgcgatg
    10321 atggaagccg catattccag tgtaatcgcg aacgttgttt tggtaccggt catggacgca
    10381 aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc cgccatgttg
    10441 gccggagaca gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc aaccatcagc
    10501 atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct
    10561 ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga gaagttaaag
    10621 gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc atgaccggtt
    10681 aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta agggatcagc
    10741 gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct catcacttta
    10801 accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag gaacagcgcc
    10861 acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt cataaaggta
    10921 ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac
    10981 gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg
    11041 gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata aaatgcgatg
    11101 gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc tgcgcggata
    11161 gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt ccatgtggta
    11221 tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc tgaagtactc
    11281 atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg agagacgcga
    11341 gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg aatatattgg
    11401 tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc ggtgacgact
    11461 ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct gaccagcacg
    11521 atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg ggagacatca
    11581 caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa
    11641 gccgccatgc cgtgaacgtt ttcagactgt acgccaataa cacgaatggt cgggttaata
    11701 gatttaattg ccaccgcaat accagcaatt aaaccgccac caccaattgg cacaatcacg
    11761 ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg gccagcaatc
    11821 actttcggat catcgtaagg tgggataaaa atacggcctt ccatttcgac aatttcgctc
    11881 actttagcga tagtgtcgtt gaaattatca ccatgcagaa cgacttctgc ggagtagtcg
    11941 cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt accgtcgata
    12001 cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga acaggccact
    12061 acacctttac gtttttccgc atcagtcagt gaacttaatt tattaaatgc gccacgaatt
    12121 ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc tttgcaacgt
    12181 tcactaaaat agtttgagcg aggcatgcct gttttataaa ttcgcccagc cagtcgttgt
    12241 ttcgcttcaa taatgtcatc aatagcaacc ggcagatcgt atgtaatatg cattataaaa
    12301 cctcttcgcc tgaattaaat atgtagg
//
LOCUS       DAZFDP010000138        12313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFDP010000138 DAZFDP010000000
VERSION     DAZFDP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..400)
                     /locus_tag="ACHJ1G_003926"
     CDS             complement(47..400)
                     /locus_tag="ACHJ1G_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4765694.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHJ1G_003927"
     CDS             complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHJ1G_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4765695.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2228..2521)
                     /locus_tag="ACHJ1G_003928"
     CDS             complement(2228..2521)
                     /locus_tag="ACHJ1G_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4765696.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHJ1G_003929"
     CDS             2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHJ1G_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4765697.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4069..4545)
                     /locus_tag="ACHJ1G_003930"
     CDS             complement(4069..4545)
                     /locus_tag="ACHJ1G_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4765698.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4882..6318
                     /gene="aspA"
                     /locus_tag="ACHJ1G_003931"
     CDS             4882..6318
                     /gene="aspA"
                     /locus_tag="ACHJ1G_003931"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4765699.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHJ1G_003932"
     CDS             6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHJ1G_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4765700.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7853..8191
                     /gene="cutA"
                     /locus_tag="ACHJ1G_003933"
     CDS             7853..8191
                     /gene="cutA"
                     /locus_tag="ACHJ1G_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4765701.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHJ1G_003934"
     CDS             8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHJ1G_003934"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4765702.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9901..10476
                     /locus_tag="ACHJ1G_003935"
     CDS             9901..10476
                     /locus_tag="ACHJ1G_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4765703.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10583..10658
                     /locus_tag="ACHJ1G_003936"
     tRNA            10583..10658
                     /locus_tag="ACHJ1G_003936"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10616..10618,aa:Phe,seq:gaa)
     gene            10856..12121
                     /locus_tag="ACHJ1G_003937"
     CDS             10856..12121
                     /locus_tag="ACHJ1G_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4765704.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
BASE COUNT         2935 a         3209 c         3047 g         3122 t
ORIGIN      
        1 agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
       61 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
      121 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
      181 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
      241 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
      301 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
      361 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
      421 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
      481 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
      541 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
      601 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
      661 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
      721 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
      781 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
      841 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
      901 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
      961 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
     1021 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     1081 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     1141 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     1201 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     1261 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     1321 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     1381 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
     1441 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     1501 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     1561 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     1621 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     1681 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     1741 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
     1801 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     1861 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
     1921 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
     1981 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     2041 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     2101 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     2161 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     2221 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     2281 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     2341 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     2401 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     2461 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     2521 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     2581 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
     2641 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     2701 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     2761 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
     2821 gggctggccc aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     2881 gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
     2941 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     3001 gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc
     3061 ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc
     3121 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     3181 acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa
     3241 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
     3301 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
     3361 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
     3421 gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca
     3481 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
     3541 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     3601 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
     3661 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
     3721 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg
     3781 agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
     3841 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
     3901 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
     3961 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa
     4021 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     4081 atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa
     4141 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa
     4201 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc
     4261 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
     4321 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac
     4381 gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac
     4441 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     4501 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     4561 tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     4621 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     4681 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     4741 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     4801 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     4861 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     4921 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     4981 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     5041 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     5101 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     5161 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     5221 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     5281 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     5341 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     5401 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     5461 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     5521 gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     5581 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     5641 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
     5701 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     5761 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
     5821 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     5881 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     5941 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     6001 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     6061 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     6121 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     6181 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     6241 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     6301 gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg
     6361 tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa
     6421 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg
     6481 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
     6541 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
     6601 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
     6661 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
     6721 tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc
     6781 gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta
     6841 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
     6901 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
     6961 ctgctggcgg ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct
     7021 gacgatccga tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag
     7081 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
     7141 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
     7201 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
     7261 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
     7321 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
     7381 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
     7441 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
     7501 ccgatggccc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg
     7561 tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac
     7621 acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
     7681 ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga
     7741 ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
     7801 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
     7861 tgaagaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
     7921 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
     7981 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
     8041 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
     8101 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
     8161 cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt
     8221 gccggattat tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct
     8281 tttgattttc agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac
     8341 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
     8401 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
     8461 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
     8521 taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
     8581 agcgaagtgg tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag
     8641 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
     8701 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
     8761 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
     8821 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
     8881 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
     8941 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
     9001 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
     9061 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
     9121 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
     9181 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
     9241 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
     9301 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
     9361 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
     9421 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
     9481 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag
     9541 ttaaatcagg cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac
     9601 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
     9661 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
     9721 gcactgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
     9781 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
     9841 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
     9901 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
     9961 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
    10021 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
    10081 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg
    10141 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
    10201 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
    10261 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
    10321 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
    10381 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
    10441 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
    10501 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
    10561 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
    10621 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
    10681 cggaggtccg tttttcgttt cagaacacca tgatttaagt gttcttcctc caaatcaatt
    10741 ctaccgaact caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc
    10801 ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc
    10861 actgactgac gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga
    10921 cggggctggc atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta
    10981 tcgtattctg ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc
    11041 cgaagctcgt acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg
    11101 cgaacagaaa agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc
    11161 acgacgctgg catgccagta ataaacaatg ggcacaatca cacagcgata aagtactcaa
    11221 aagcctcgag acacacgtat tcccctttat cggcaatcgg gatatcacaa cactcaatac
    11281 cccggacctg cttatccctg ttcgtgctgc agaagctaaa caaatttatg aaatcgccag
    11341 tcgtctgcag caaagaatat ctgccgtaat gcgttatgcc gtacagtctg gcatcatcag
    11401 atataatcct gctctggata tggctggcgc attgactacg gtaaaacgcc agcatcgccc
    11461 cgctcttgat ctttctcgcc tgcctgaact tttgtcgcgt attggcagtt acaaggggca
    11521 acctgtcacc cagcttgccg ttatgctgaa tttactggtt tttattcgtt ccagtgaact
    11581 cagatacgcc cgctggtctg aaattgatat tgacaatgcc atgtggacta ttccagccga
    11641 acgcgaacct ctgccaggcg taaaattctc acaccgggga tccaagatgc gaacaccaca
    11701 tcttgtgcca ctcagcaaac aggttgtagc catactggca gaacttcaga catgggctgg
    11761 tgaaaatggt ctgatcttta cgggagcaca tgacccacgt aaaccaatca gtgaaaacac
    11821 cgtaaataaa gccctgaggg ttatggggta tgacacaacc caggatgtct gtggtcatgg
    11881 attccgggcg atggcatgta gtgcgttaat agaatcaggt ttgtggtccc gcgatgcagt
    11941 tgaacgtcag atgagccatc aggaacgtaa cggtgtacgt gctgcttaca ttcataaagc
    12001 agaacatctg gaagaacgtc gactgatgct acagtggtgg gccgattttc tggatgcaaa
    12061 cagagaaaga tttatcagtc catttgaata tgcaaagatt aataatccat taaaacagta
    12121 atcatcccgg gcaaatgccc gggaacctac tccaggatta ttttctttgt taaaaaaaca
    12181 gacaaaacgg tattaacttc gtaagcgtac agcgagggcc gtattgacgg ggatgtgtta
    12241 ttcagctggc agtgctatgc gccacggaag cagttcgctg acccggttga ccggccagtc
    12301 tgctatgacg cca
//
LOCUS       DAZFDP010000139        12273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFDP010000139 DAZFDP010000000
VERSION     DAZFDP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..186)
                     /locus_tag="ACHJ1G_003938"
     CDS             complement(34..186)
                     /locus_tag="ACHJ1G_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4765705.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(451..1185)
                     /gene="cysH"
                     /locus_tag="ACHJ1G_003939"
     CDS             complement(451..1185)
                     /gene="cysH"
                     /locus_tag="ACHJ1G_003939"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4765706.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1259..2971)
                     /gene="cysI"
                     /locus_tag="ACHJ1G_003940"
     CDS             complement(1259..2971)
                     /gene="cysI"
                     /locus_tag="ACHJ1G_003940"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4765707.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2971..4770)
                     /gene="cysJ"
                     /locus_tag="ACHJ1G_003941"
     CDS             complement(2971..4770)
                     /gene="cysJ"
                     /locus_tag="ACHJ1G_003941"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4765708.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5089..5451
                     /gene="queD"
                     /locus_tag="ACHJ1G_003942"
     CDS             5089..5451
                     /gene="queD"
                     /locus_tag="ACHJ1G_003942"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4765709.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5529..6800
                     /gene="ygcN"
                     /locus_tag="ACHJ1G_003943"
     CDS             5529..6800
                     /gene="ygcN"
                     /locus_tag="ACHJ1G_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4765710.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6791..7051
                     /gene="ygcO"
                     /locus_tag="ACHJ1G_003944"
     CDS             6791..7051
                     /gene="ygcO"
                     /locus_tag="ACHJ1G_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4765711.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7068..7643
                     /gene="ygcP"
                     /locus_tag="ACHJ1G_003945"
     CDS             7068..7643
                     /gene="ygcP"
                     /locus_tag="ACHJ1G_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4765712.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7791..8651)
                     /gene="ygcQ"
                     /locus_tag="ACHJ1G_003946"
     CDS             complement(7791..8651)
                     /gene="ygcQ"
                     /locus_tag="ACHJ1G_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4765713.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8648..9427)
                     /gene="ygcR"
                     /locus_tag="ACHJ1G_003947"
     CDS             complement(8648..9427)
                     /gene="ygcR"
                     /locus_tag="ACHJ1G_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4765714.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9405..10814)
                     /gene="ygcS"
                     /locus_tag="ACHJ1G_003948"
     CDS             complement(9405..10814)
                     /gene="ygcS"
                     /locus_tag="ACHJ1G_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4765715.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DYIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10836..>12273)
                     /gene="ygcU"
                     /locus_tag="ACHJ1G_003949"
     CDS             complement(10836..>12273)
                     /gene="ygcU"
                     /locus_tag="ACHJ1G_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4765716.1"
                     /translation="AIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLPI
                     PAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQI
                     INIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFST
                     LYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITEVTVKIF
                     KFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHFADGKCV
                     LIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQI
                     LKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMY
                     FVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGS
                     AWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2790 a         3386 c         3154 g         2943 t
ORIGIN      
        1 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
       61 tgccttaaac tccataccac gttctctgat actcaactca cacaacgagt cacctaccat
      121 cagcgtgaat cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt
      181 cagcatgtct tgcctccctg caaagaagag actaacattc gaattgtttg ggttcgaacg
      241 taagcctcag gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat
      301 gcttaacgcc agacagcctc aagcacccga cgccattcta tacctgataa ttcttccagc
      361 ggcgttcacg tttaaaatca ggaatactgc tcgcaaaata aaaacgcccc catcatttct
      421 gacagaggcg tttaatttat ccggcaatat ttacccttcg tgcaatccac attccctttt
      481 taagccaaag aaacgtgttt cttcttccga catgccaggt tcccatttac gggttgtatg
      541 ggtatcgccc accgataaat atccttcgtc ccataatggg tgatatttca ggccatgttt
      601 ttgcagatac tgatatatcg ttcggttatc ccagtcgata atcggcagca ctttaaatac
      661 gccacgctga attgccagca ccggtaaatt ggcgcggctg ccggattgtt cgcggcgcag
      721 gccagcaaac cacgtttgcg cattcagttc tttcagcgcc cggttcatcg gttcgacttt
      781 gttgatgtca ttgtactttt caatgccttc aacgccctgt tcccacagtt tgccgtagcg
      841 tgcttcctgc caggcggcgc tttcggtagc gcggtacact ttcaggttga gcttgagttt
      901 gtccgttaac tcgtcaataa agcggtaggt ttccgggaac agatagccgg tgtcggtgag
      961 gatcaccgga atatccgggt gaatctgatt caccagatgc aggctcaccg ccgcctgaat
     1021 gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc aggcaacgcg
     1081 gccttcagcg tccagttttt ccagttcggc gttagtttct gccagcgcca gaatgcgatc
     1141 taccttcggc agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt
     1201 tgccttgcct gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt
     1261 aatcccacaa atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa
     1321 agtcgccgaa gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa
     1381 gcgacgccag gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc
     1441 caatgcggtt gccgccaaga tgcaggttgt agcgacccgg cgctttaccc accaggccca
     1501 cttccgccag catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat
     1561 gctcatcgct gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca
     1621 ggaaacgctc tgcttccgcc atcgccagcg ggcaagtcgg gaacgagacg caggccatcg
     1681 agttttcacg ctgcggcgtg acggcattca ttaacccgct ctctttggcg atcttctcga
     1741 tcttcgcttt ctcgctttcc ggtacaccgg cgatgatcag attctggttc gccgtaatgc
     1801 ggaaatcgcc tttgtggatc ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg
     1861 gataatcaag gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc
     1921 ccttaaccca gccaatacga tcgcctcgtc cggtgaactc atatggacgg atcggttcaa
     1981 atttgatccc cgcgcgacgc tccacttccg ctttaaacgt ctcaaccccc acgcgctcca
     2041 gcgtgtattt ggttttggca tttttacgat cggttcggtt accccagtca cgctgagttg
     2101 tcacgacggc ttcggccacc gccagcgtat gctccagcgg cagatagcca aactcgctcg
     2161 ccgtgcgggc gtaggttttc ttgttgccgt gttcgataga aagcccaccg cccaccagca
     2221 ggttgaagcc taccagcttg ccgttttcgg cgattgccac gaagttcatg tcgttggcat
     2281 gcagatcgat atcgttctgc ggcgggatca ctaccgtggt tttgaactta cgcggcaggt
     2341 aggtctgacc aaggatcggt tcttcatcag tagtggcgac tttttcgtgg tcgagccaga
     2401 tctccgcata cgcgcgggta cgaggcaaca gatgctcaga aatcttcttc gcccactcgt
     2461 acgcttccgc gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca
     2521 tgtcgttagc tgtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt
     2581 tgacgttctt tttcagaatg ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc
     2641 tgccatagat ggtgttttca ccggcaaatt tgtcgatcgc ctgccactgt ttagtggtaa
     2701 tcaccccacc cggcagacga cagcgaagca gcatcgcgtg gcgcggctcc agcttctgtt
     2761 cagcacgttc ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca
     2821 gcaggaagtt gtcgccctta aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg
     2881 tgccgcgcag gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga
     2941 ccactaaagg ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc
     3001 tacgcgcagc tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc
     3061 aatcacttcc agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac
     3121 ataaatgtgg gcaccatcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt
     3181 gtcttgtacg taaacttttt ctttttggtc gcgcgaccag gcaagatcga tacgtgtcag
     3241 cacgccatct ttgacgtagc gctgccactc cacctgatac aggaagtctt ccgtaaagtg
     3301 cggattacca aagaacagcc agtttttacc tggcgcttcg tcggcggcgc gttgctgcat
     3361 aaaggcgcgg aacggcgcaa taccggtgcc tgggccaatc ataatcaccg gggtttccgg
     3421 gttagcgggc aggcgaaagt tatcgttatg ttcgataaat acgcggactt cgccctcttc
     3481 ttccacgcga tccgcgagga agctggaggc accaccggca cgggctcggc cttccacgtc
     3541 gtaacgcacc acaccaacgg tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc
     3601 gatggaatac aggcgcgggg tcagcgggcg cagcagatta atcagcgctt cggcatccag
     3661 ttgcgccgga gagaaacgca ccatgtcgac aatcggcgtc gtcgcggcgt aatgctgtaa
     3721 cttcgcttta tcgcccacca gcggcagcag tgtttcgctg cgggtaagcg tggcgtaatt
     3781 ctcaacaatg ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag
     3841 aggcaacgtt ttgccctcga cggtgacagg ttcatcgcct ttcagccaca gcagttcgac
     3901 aagttctttc accagtgccg gatcgttctg ataccagacg cccagcgcgt cacccggctg
     3961 gtaacgcagg cccgagtcac ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt
     4021 acgcccggta attttctggt taacagagag gctagccacc agcggcgcgt ctttgctgta
     4081 cgggctggtg tggatttcat ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac
     4141 aggcgcacgc gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg
     4201 gtattcaaca tcggcatcga cacggtcgag caggcgttca ccacccagtt ccgccagctt
     4261 gctgtcgaaa tctttcccgg actggcagaa aaattcataa gagctatcgc cgaggctaaa
     4321 cacggcaaac gcggtgtttt ccagctttgg cgcttttttg gagaacagga acttatgcag
     4381 cgcgacggct tcttccggcg gttccccttc cccttgcgtt gacgtcacta cgatgagcag
     4441 tttttcgctg gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag
     4501 ttttgctgct aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg
     4561 cgaggcggag ataatagtta tacccggcat ttctgcggct ggcgctggcg tcgctgcaag
     4621 cgcagcaggc tgctgattga gcacgcccca gaaatagcca gaaacccagg caagctgggt
     4681 gggagttaaa tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag
     4741 caacgcggaa ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt
     4801 ttctgcgccc tgtcagcgcc cataaaacag aagagaaggt aaggttaacg gggtgaacgg
     4861 tgtggattaa agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa
     4921 tagtctttaa caacaaaata gattaaccaa cctaatgaaa aacaaatgaa tttagccaat
     4981 cattaagata aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag
     5041 cctttccggt actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg
     5101 ttatttaaag atttcacctt cgaagccgct caccgcttac cacacgtccc ggaagggcat
     5161 aaatgtggtc gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc
     5221 gatccgcata cgggctggat tatcgatttc gctgaactaa aagcggcgtt taaaccaacc
     5281 tacgagcgcc tcgatcacca ttatctcaat gatattccag gtctggaaaa cccaaccagc
     5341 gaggttttag caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg
     5401 gtgatggtaa aagaaacctg taccgcaggt tgtatctatc gcggcgaatg ataagagtgt
     5461 gtcggcggtc aatttccctt aagtaacgct atgttagggt gttgtgttct ggatatctgg
     5521 ggcatgacat ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg
     5581 cttgcgcgtt acgctgcgcg cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa
     5641 tccccggcag caaaaatctt tccggcgggc ggttatatac ccatgcactc gcggaactcc
     5701 tcccgcaatt tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc
     5761 tgttaacgcc ggatggcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga
     5821 gtgtattacg tgcacggttc gatccgtggc tggttgccga agccgaagaa gaaggtgtcg
     5881 aatgcatccc cggagcgacg gtggatgcac tgtatgaaga aaacggcagg gtgtgtggtg
     5941 tcatttgtgg tgacgatatt ctccgtgccc gttatgtggt gctggcagaa ggtgccaaca
     6001 gcgtcctggc tgagcgtcac gggttagtga ctcgtcctgc tggtgaagcg atggcgttgg
     6061 ggatcaaaga agtgctgtcg ctggaaccat ccgctattga agaacgtttt catctggaga
     6121 ataacgaagg cgcagcgttg ctgttcagcg gcaggatctg tgatgactta cccggcggcg
     6181 catttcttta tactaatcaa caaacgctct cgttagggat tgtttgcccg ctctcttccc
     6241 ttacgcaaag tcgtgttccg gcaagcgagc tgctgactcg ctttaaagcg catccggcag
     6301 tgcgcccgct tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag
     6361 gtggcttgca cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg
     6421 cgttgcgcag ttgcgtcaat accggaattt ccgtgcgcgg catggatatg gcgctgactg
     6481 gcgcgcaggc ggcggcacaa acgctgataa gcgcctgcca gcaccgcgag ccgcaaaatc
     6541 tgtttgcgct ttatcatcac aacgtagagc gcagcctgct atgggatgtt ctacaacgtt
     6601 atcagcatgt tccggcgctt ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa
     6661 tgcaggatat ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc
     6721 agttattctg gcgtcattta cgtcgtcatg gcctgtggca tctggcgggc gatgttatca
     6781 ggagtctgcg atgtctgtag cccataatct ctggcgcgtt gctgatgcgc cgcacattgt
     6841 tccggctgac tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggcagg
     6901 tcttttttcg ctcacaccgg aaggtgactt acgcgttgat tatcacggct gcctggagtg
     6961 tggcacctgc tgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc
     7021 cggattcggc atcacctacc gctttggata aataataagg ataatttatg cccctcttac
     7081 acctgctccg ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg
     7141 caatcgactc tgaatgccag tttatttccg tgttgtacgg caatatctgc accatcagta
     7201 atatcgttaa aaagattaag aacgccggga aatatgcttt tattcatgtt gatttgctgg
     7261 aaggcgcgtc aaataaagaa gtcgtgattc agtttttgaa actggtgacc gaggcggacg
     7321 gcattatcag taccaaagcc tcaatgttga aagcggcaag agcggaaggt tttttctgta
     7381 ttcatcgcct gtttattgtt gattcaattt cgtttcacaa cattgataag caagttgcgc
     7441 aatcgaatcc ggattgtatt gagatcctgc caggctgtat gcccaaagtg ctgggctggg
     7501 tgacagagaa aatccgccaa ccgctgattg ccggtgggct ggtgtgcgat gaagaagatg
     7561 cgcgtaatgc gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggggtctgga
     7621 cgttagcgaa aaaattactt tgacgggata atcgtaacca attgaatttg gtttgatttg
     7681 taggccgcac gccacatccg acattcagcg cctgatgcga cgcttgacgc gtcttatcag
     7741 gcctacaagt ccccaggcca gaaccgtagg caggatcatc cggcaacacg ttattggcaa
     7801 tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgcca
     7861 acatctgcct gtgaaaatac cgcagcgctg gcgtcatgat taatcgccac cacaaacttg
     7921 ctattgcgta cgcccgccat taacgctgct gcgccagaag cgccaacaac aatacacacc
     7981 tcaggtgcca gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc
     8041 atcactcgcg cccgactgta gcccacttcg gctcccagct tttctgccag catagcgatt
     8101 tcctggttat ctgcctcacc gccctgcccc accaccagca cccgtctggc ctcagctaat
     8161 gggtcacggg taacattttt cagatcctct gtgctaacca gccagtcggg aagcgcgccg
     8221 gggacaatgt tcagttgctg cataccagag ggcaacgtgg cgtttttcac tgctcccgcc
     8281 tgacgcgcca gggaaaggca tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca
     8341 tttccccagt gggatttgcg cacgcttacg gttggaatat ctaacgaagt cacctggcag
     8401 atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc
     8461 ggcggaaaga gaacaacgtc cgcaggcgtt cgctgccact gttccaccaa cgcatccagc
     8521 acctgttccg ccactacagg ctgaggttca atctgccagt gcgccagggt acaaccgctg
     8581 aaatcctgcg ccgccagcca gcttgctatt gcggcattct cctgatttat tgtcacaatt
     8641 gcgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgagcttttt
     8701 ctgctaccgt ttgcccatca atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt
     8761 gcagacactg catagctggc atttcggcag caacagtttt acggataatt tctgctttcc
     8821 ccgcagccat tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca
     8881 cggcaatcac cgcaggcaag cgaacccggc aacaacgcaa accatgttca atacgctgtt
     8941 cgagggtgat aaacagcgcg tcgagagtga aacgctccac ctgggtgaag cagggccagc
     9001 ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc
     9061 cggtaattat cagatccagc ggattctgat gctgccattc ggcaatatgg cgagcgacaa
     9121 attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa
     9181 gagccatgag atagcgcagc cagtgcaatg cccgttcatc ccccatgctc aacgcggtta
     9241 aagacatcgg cgtaccgttt tttctctgcg ccaacagcag cgcggcagcg gcctgttcat
     9301 cagcaccgag taaacttcgc agtagcgaaa tatccggtcc gcttttaccc tgagccgccg
     9361 cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa
     9421 tgttcattcg cacctcctac atttcccgcc gccaccagcg ggagggcttt agtttctggt
     9481 gcccatagcc aggtcacaac aaaaccaacc aacaacactg tcgccaggag caataaggtg
     9541 acttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgcca
     9601 agtcgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt
     9661 tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca
     9721 aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccattactac cagcgttgcc
     9781 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
     9841 aatcccagca gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca
     9901 tcttccagac caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
     9961 attacgaggc agacaaagaa gacgctgtta aacgccgtgc gccgccagta acgcgaagag
    10021 aacaaggttt tgatgtgttt atgggtcgcc gttaccactt catcgcccag taaaacatgg
    10081 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc
    10141 cagcgtggtg attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct
    10201 gccgatgcca gtagccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca
    10261 atacttgcca gcacatagcc tacggtccac accacgctga atgcgcccag caaaataccg
    10321 cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac ctactgaata atcgcctccc
    10381 agaccaatgc caatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat
    10441 tgtaaaaacg aagcaagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca
    10501 atatagtcgg agatccaccc aagaaccagg ctgccaagga acaaaccaag gagcgccgag
    10561 ccgccgatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg
    10621 gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
    10681 gcaatgcggc agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc
    10741 atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
    10801 caacgccccg tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta
    10861 cccgtattca taatgccatt aggatcgaac tgctttttca gcccttccag caacgcccac
    10921 gcgctgccgt gttccagttt gctccagtga acgcgatgtt taccgatacc gtggtggtgc
    10981 accatcgaac cgccgaggcg aatggtttct tcacagatga tcttgttgag cggattgtgg
    11041 tacttgtcga tttcctcttc cggcttacag tcaacgacgt tgtaatcgta gacgaagtac
    11101 atgttggtgc cgttctgata gctatgagag gaatgaccgc ccagcatggt gatgtcgtcg
    11161 gcgtgcggga actcagtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag
    11221 ctccagcagc cggacacttc agtggtaaag cccatgttgc cggttttgag gatctgcaca
    11281 cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagt
    11341 ttgctgtcca cgcgctggca ctgcgggtag cgggcaacga tttccgcaat cccttcgccc
    11401 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacaca ttttccgtct
    11461 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
    11521 cgataccctt ccaccatgac ttcacgcagg atgttgaagc cggttttcat gtcttccagg
    11581 atatagccgt agaagaggtt gttttccggg gtgaatttaa agattttcac tgttacttca
    11641 gtgatatagc aaagtgcacc ttcgttaccg atgatgatgt ggcgaatgtc cgtgccagcc
    11701 gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat cagccaatac tgcttccaga
    11761 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
    11821 gctaccaggc cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa
    11881 cctttttcac gcaacgcatt ttccagcact tcctggggaa cgccacattg cgccgtcgcc
    11941 tgcatattct caatatcaat attaatgatt tgattcatgg cggaaccgtc gagcaccacc
    12001 gagttttcta caacagtttc cagcccacct tcagtggcgg aagcgccggt acgcggcaca
    12061 ccgttaattt tgtgcgcatt cataaaattc agcacacggg agacttgttc tgtggaaccg
    12121 agttttacga ccgctgccgg aatcggcaaa gtataaatac catgaatatc cggaaattta
    12181 cgaaaacggt caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca
    12241 ccaacaattt cctttagttg gtcgacaatc gct
//
LOCUS       DAZFDP010000140        12150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFDP010000140 DAZFDP010000000
VERSION     DAZFDP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..954)
                     /gene="yihR"
                     /locus_tag="ACHJ1G_003950"
     CDS             complement(52..954)
                     /gene="yihR"
                     /locus_tag="ACHJ1G_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO4765717.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1092..2333)
                     /gene="yihS"
                     /locus_tag="ACHJ1G_003951"
     CDS             complement(1092..2333)
                     /gene="yihS"
                     /locus_tag="ACHJ1G_003951"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO4765718.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2350..3228)
                     /gene="yihT"
                     /locus_tag="ACHJ1G_003952"
     CDS             complement(2350..3228)
                     /gene="yihT"
                     /locus_tag="ACHJ1G_003952"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO4765719.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3252..4148)
                     /gene="yihU"
                     /locus_tag="ACHJ1G_003953"
     CDS             complement(3252..4148)
                     /gene="yihU"
                     /locus_tag="ACHJ1G_003953"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4765720.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4316..5212
                     /gene="yihV"
                     /locus_tag="ACHJ1G_003954"
     CDS             4316..5212
                     /gene="yihV"
                     /locus_tag="ACHJ1G_003954"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO4765721.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5246..6031
                     /gene="csqR"
                     /locus_tag="ACHJ1G_003955"
     CDS             5246..6031
                     /gene="csqR"
                     /locus_tag="ACHJ1G_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4765722.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6130..6729
                     /gene="yihX"
                     /locus_tag="ACHJ1G_003956"
     CDS             6130..6729
                     /gene="yihX"
                     /locus_tag="ACHJ1G_003956"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4765723.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEVF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6723..7595
                     /gene="yihY"
                     /locus_tag="ACHJ1G_003957"
     CDS             6723..7595
                     /gene="yihY"
                     /locus_tag="ACHJ1G_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4765724.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEDDDEP"
     gene            7592..8029
                     /gene="dtd"
                     /locus_tag="ACHJ1G_003958"
     CDS             7592..8029
                     /gene="dtd"
                     /locus_tag="ACHJ1G_003958"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4765725.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8074..9015
                     /gene="fabY"
                     /locus_tag="ACHJ1G_003959"
                     /gene_synonym="yiiD"
     CDS             8074..9015
                     /gene="fabY"
                     /locus_tag="ACHJ1G_003959"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4765726.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9079..9987)
                     /locus_tag="ACHJ1G_003960"
     CDS             complement(9079..9987)
                     /locus_tag="ACHJ1G_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4765727.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10216..10527
                     /locus_tag="ACHJ1G_003961"
     CDS             10216..10527
                     /locus_tag="ACHJ1G_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4765728.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10528..10818
                     /gene="nadS"
                     /locus_tag="ACHJ1G_003962"
     CDS             10528..10818
                     /gene="nadS"
                     /locus_tag="ACHJ1G_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4765729.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11423..11641
                     /gene="yiiE"
                     /locus_tag="ACHJ1G_003963"
     CDS             11423..11641
                     /gene="yiiE"
                     /locus_tag="ACHJ1G_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4765730.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            11860..12102
                     /gene="yiiF"
                     /locus_tag="ACHJ1G_003964"
     CDS             11860..12102
                     /gene="yiiF"
                     /locus_tag="ACHJ1G_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO4765731.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT         2979 a         2939 c         3202 g         3030 t
ORIGIN      
        1 ctttagcgag tgaaatgttc ttcaatgatt ttgactgcta atcaatacaa gttattttct
       61 ctgtccgtaa atgttaaaaa acaggcggtg cggttttcct ggttccagta ataataagtc
      121 gatacctgaa ttaaaggcgt taggcgggca actcataggt tcaactgcca gtccctgacg
      181 ctgtaatttt tcaccgctat aaatttgtac ccagggttga tcgcaacaaa ggctaaccga
      241 aagcgcctgt tgtggatggg taatggttat ttcccacaga tcattagcgg ttttaaaggt
      301 gtggtcgatt tttgtcgcgg ctattgtttt cgcctgcgtg aaatttaaat ccagctcatc
      361 aacgtggtga agcgttgtcg gatttacgtg ttcatcaacg gcataaacct ggtttgccgg
      421 aagctggaac agatattcat caacagatgt taagttgcag gttaaataag tatgaatacc
      481 cacgccataa ggcgctgcga cagtgccgat attctggctt gcgatctcaa cggaaagacc
      541 cgtgtgtgcg ttgagcgaat aaacaacttg tgaagccagc ataaacggat agccgtaact
      601 gggcggtaga aaagctgtca gggtaacgct ggtggcggtt aattcactaa tttgccagtc
      661 tcgccaggca agcagcccgt gaatagcggc ttttgaacta tgttcattaa tgggtaactg
      721 atattcctgc ccctgatagc ggtaacagcc gttggcgatc cggttcggcc acggaataag
      781 cactttaccc agatgcgcca gcggcatctc ttcgggtttg tgcgggatga ctaaatgaca
      841 accctgaaag gttaattcag ccagaccagc accgacggtc acaatggtcg cgtgataatc
      901 ccccgcggca agtgaaacgg tctgtccact gcaatgcata ttggtgattt gcatacaagg
      961 caccttaatt aacgacatac agcctcccgt gaggctgatg tcaaacccaa aaactgcacc
     1021 ggacggtccc ctcgcccctt tggggagagg gttagggtga ggggaaaacc cgcaccctat
     1081 tttcagatca gttatttcgc attaatatcc agcagacccg ctgcaaccgc tggagccatg
     1141 ccgggcgcta acgggatacg cgggatcacc aggcaatgca gcaggtgata aatatcctgt
     1201 ttgccgtccc agactttggt ggtgacctta ttgtccgcat ccagctcctg ccaccaggaa
     1261 ccattttcat agtccatcag gtacttaatg cagtactccc accatgtttg ataccaggtt
     1321 tcatactggc gatcgccagt gacggtgtag agcgcgtagg ccgtacccat tgcttcgacg
     1381 ataggccaac gtacacgttc gcggaccacc ggttttcctt cccagtcaac ggtataaaca
     1441 atcccgtccg cgccatcggg tgcccaggca tcgcgcacgg tggcgttaaa cagacctttg
     1501 gcatcttcta gcagccatgc tggtggttgt tcgcaacggg cttccagggc agcatggatg
     1561 tgcagcatta aacggcccca ttcgatccag tggcctggtg tacctccgaa cgcgcggaag
     1621 cgatgcgccg ggttatcttt gttgtaatcc ggcagcggat tccactgggt atcgaagtgt
     1681 tcgttaacgc gataatgatt atttctggcg acgtcgtgga taatcacgga agccacgcga
     1741 atcgcgcgat ccagccattt tttgtcgtga gtgacgtcat aaacaatcaa gaaagcttcc
     1801 accgcgtgca tattggcatt gccgccgcgg tactcttcgg ttttgctgaa ggcttcgtcc
     1861 caggattcca ggcacatctg ctcttcttcg ctccagaaat atttctcgat aatttcaatg
     1921 gtgtaatcga gcagcttgcg cgcttccggg tgacccgttg tgacggcgct ggcggcaccc
     1981 agcagagcaa agaagtgttg atagccctgt ttggaggcat ccaccacgcc ctcgtcattt
     2041 acgcaggcat accagccgcc atatttttta tcacgcagtg cgccgttcat ggctttgata
     2101 ccgtgatcaa ccaacgagta agcgccaggt cgacccatcg ccgcagcaac ggaataaacg
     2161 tgtaacatac gagcggtgat ccacagatgg gtgcccatct cttctttgat ttgcccttta
     2221 ttgcctaacc agccaaaacc agtcggcact actgaatttt taccaaaatc aaagatgcgg
     2281 tcggtttcct gttccagcca acggttgtgg cttagggtgt taaaccattt cattcctttt
     2341 atcctcatct tagcggcgtt tagccatcat ttcgtcgacg atatcgccaa gttgttgtaa
     2401 tttcggtgcg caaacgtcac gcagcatcag ctcgttgtct ggtaaaccga cgaccgatgc
     2461 ccagactgca cggcctgcca ggaatcccga tgcgcccgcc gtcattgcca cgcgtacggc
     2521 acgcgggaac agtttttcgt cgacaccgga agagaggatc acccatggca tattgatatg
     2581 gtcattcaga cgttgtgaag cacagagaag ctcttgttgc ggacctttgc cataaagcgg
     2641 catttcaact ttgtagaggt cagcgccact gtcgcccagc tctttggcgg catcgatgat
     2701 cgcttgttcg cgatcgaatt tatcgccacg acgcggtggg cggacgactg gctcaatgat
     2761 gcttaccaga ccgtgtgagt ggcacagttc gttgaactct ttcaccatat ccagacgttg
     2821 ctgcgcatct tcatcactac gccacagcac cagcagtttt aaggctttgc caccgtcttg
     2881 tttgatctgt agcggattga ttttgcggtc aataaccacg ctatcgaccg gaataccgtt
     2941 gccaggaatg aactcatcgg cggcgacaat catggcgcaa cttttggcaa tcgcgttttg
     3001 ctcaaccacc tggcggtagc agaattgttg atctaccaga atcgccgagg cataaggcga
     3061 gagggccttt gcagcgttaa ctttgaaatc agttaaaacg ctatcggcta ccggagcggg
     3121 tgccccagcc gcggcaaaca tcatgcgcat ggcttcgcgc tgatcgaccg ccagcatggc
     3181 aaaaccgccc gatgcgcgcg taatgtcgtt gatggtgtac ttattcattc atttattcct
     3241 ttatccagtc gttacatttt tactttggca gtcatcccgg cactgacacg gacctgttcc
     3301 agaatggcgg accagtcctg gcgaccgcga cccgctgcgc gcgcctggct ataaacctcc
     3361 cgtgaggcgg cccccagcgg cattggcaca tgcagctggt tggcgacatc aagggcgatg
     3421 ccaagatcct tatgggcaag atcgatcatg aaggcgggag aaagatctcc gctgaggact
     3481 ttgtttggcc aggaagtggt gaagtgacct ttaccggcgg cggtaccgct catcactttg
     3541 acggcaacat cgaagggaag attcagggct tcgcacaaaa cggcggcttc tgccgaaagc
     3601 gcattgagcg cgatgctcat gtagttgttg atgagcttaa cgcggatccc catgcccgga
     3661 ccgccagcgt tgatcaactc actgcccatc gccatcagga tcggtgtggc acgttcaact
     3721 tgttcagcgg tgccgccagc cagcagtaac agagtaccgg taatggcatt tgcagaagta
     3781 cggcctaccg gaacatccat catgctgaag cctttggctt gcatatcggc aatcaattta
     3841 tcggtttgca gcggatggat ggtggacata tcaatgacca gcgcatcggt agataagcct
     3901 tcgcaaacgc cgttttcacc gaacaacacg ttgcgcacca gatcgccatt cggcaacatg
     3961 gtaatgataa attcggcatc tttagcggcc tgcgccgggt tggcggcggg agtcgcacct
     4021 ttgtctacca gatgccgcac agcttcggcg ttcacatcaa agacgcgaag ttgatgccct
     4081 tgctgcaata aattgctcgc cattggcgaa cccatttgtc ctaaaccgat aaacgcgatt
     4141 gctgccataa ccctctcctg aatacagtga tgtcactttt tgtcatttat gacatgcttt
     4201 gcttgtctgt ttttgatcgt atttgtaatt tatcgtcaaa aaattgacag ccgtcacttt
     4261 ttaaacaatt ggtgaaatta aaatgaacgc atcccaaaat gtttaaggaa tgaccatgat
     4321 tcgtgttgct tgtgtaggta taaccgtgat ggatcgcatc tattacgtgg aagggttacc
     4381 gacggagagc ggtaaatacg tggcgagaaa ttatacggaa gttggtggcg ggccagcggc
     4441 gacggcagcg gttgcggcgg caaggctggg ggcgcaggtc gattttattg gtcgcgtagg
     4501 tgatgacgac acgggcaaca gcctgctggc agagctggaa tcctggggcg ttaacacccg
     4561 ttacaccaaa cggtataacc aggcgaaatc ttcgcaatcc gccatcatgg tggataccaa
     4621 aggcgagcgg ataatcatta actaccccag cccggatctg ctgcctgacg cagagtggtt
     4681 ggaggaaatt gatttctctc agtgggatgt tgtgctggca gatgtacgct ggcacgacgg
     4741 cgctaaaaaa gccttcaccc tggcccgtca ggcgggtgtg atgaccgttc tggacgggga
     4801 cattacgccg caggatatca gtgagctggt ggcattaagc gatcacgcgg ccttttcaga
     4861 accgggtctg gcgcgcttaa cgggcgtgaa agagatggcc agtgcgctaa aacaggcaca
     4921 aacgctcaca aatggtcatg tctatgtgac ccagggtagc gcaggctgcg actggctgga
     4981 aaatggtggg cgtcagcatc aaccggcctt caaagttgat gtggtagata ccacaggtgc
     5041 gggtgatgtt tttcacggcg ctttggcggt ggcgctggca acaagtgggg atttagcgga
     5101 gtcagtccgc ttcgccagcg gtgtagcggc gttaaaatgc acacgtcccg gtggacgagc
     5161 cgggatccct gactgtgatc aaacccgatc ttttttgtca ctttttgtat aaaatgccag
     5221 ggtgatggtt tttcgaggaa ttttcatgag ccttaccgaa ctaaccggta acccgcggca
     5281 cgaccaactc ctcatgctga tcgccgagcg tgggtatatg aatattgatg agctggcaaa
     5341 tctgctggat gtctccacgc agacggtccg ccgggatatt cgtaaattaa gcgagcaagg
     5401 cctgattacg cgccatcacg gtggcgcggg tcgggcttcc agcgtcgtta atacggcgtt
     5461 cgagcagcgt gaggtttcgc aaaccgagga aaaaaaagcg attgccgaag cggtggcaga
     5521 ctatattcct gatggttcaa caatatttat caccattggt acgactgttg agcatgttgc
     5581 ccgggcgtta cttaaccata atcatttgcg gataatcacc aacagcctgc gtgtggcgca
     5641 tattctttac cacaacccgc gctttgaagt gatggtgccc ggcggtacgt tgcgctctca
     5701 taatagcggg atcattggcc cttcagcggc gtcctttgtg gctgattttc gtgctgatta
     5761 tctggtaaca agcgttgggg cgattgagag cgatggcgcg ttgatggagt ttgatgtaaa
     5821 cgaagctaac gtggtgaaaa cgatgatggc gcacgcgaga aatattctgc tggtcgccga
     5881 tcacactaag tatcatgctt cggcggcggt tgaaattggt aacgtggcac aggtcactgc
     5941 gctctttacc gacgagctgc cgcccgctgc gctaaaatca cgcttacaag acagccaaat
     6001 tgaaatcatt cttccccagg aagacgcgta gatttttgtg accgttaacc tggcttcata
     6061 ccttgccaca tagccaaacc catcctttcc cgctacagtt aatttcttgt ggcgcgaaag
     6121 gaggcaaaaa tgctctatat ctttgattta ggtaatgtga ttgtcgatat cgactttaac
     6181 cgtgtgctgg gagcctggag cgatttaacg cgtgttccac tggctacgct taagaagagt
     6241 ttccacatgg gggaggtgtt tcatcagcat gagcgtgggg aaattagcga cgaagcgttc
     6301 gcagaggcgc tgtgtcatga gatggccctg ccgctaagct acgagcagtt ctctcacggc
     6361 tggcaggcgg tgtttgttgc gctgcgcccg gaagtgatcg ccatcatgca taaactgcgt
     6421 gagcaggggc atcgcgtggt ggtgctttcc aatactaacc gcctgcatac caccttctgg
     6481 ccggaagaat acccggaaat tcgtgatgct gctgaccata tctatctgtc gcaagatctg
     6541 gggatgcgca aacctgaagc acgaatttac cagcatgttt tgcaggcgga aggtttttca
     6601 cccagcgata cggtcttttt cgacgataac gccgataata tagaaggagc caatcagctg
     6661 ggcattacca gtattctggt gaaagataaa accaccatcc cggactattt cgcgaaggtg
     6721 ttatgctaaa aaccattcag gacaaagcca ggcaccgtac ccgtccacta tgggcctggc
     6781 taaaactact ctggcaacgt attgatgagg acaacatgac aaccctggca ggtaaccttg
     6841 cctatgtgtc gttgctctca ttagtgccgc tggttgccgt tgtttttgcg cttttcgccg
     6901 cttttcccat gttttccgac gtcagcattc agttacgtca ctttattttt gccaactttc
     6961 tgcctgctac tggcgatgtt attcagcggt atatcgaaca atttgttgcc aattccaaca
     7021 agatgaccgc cgttggggcg tgcgggctga tcgtcaccgc gttattgctg atgtactcca
     7081 tcgatagcgc gttgaatacc atctggcgca gtaaacgagc gcgaccaaaa atttactcgt
     7141 tcgccgtgta ctggatgatt ttaacgctgg ggccgctgct ggcaggggcc agtctggcga
     7201 tcagttccta tttgctctcc ctgcgctggg cgagcgatct caatactgtc atcgataacg
     7261 tgctgcgtat ttttccgctg ctgttgtcgt ggatctcctt ctggttgctg tacagcattg
     7321 ttcctaccat ccgcgtacct aaccgcgacg cgattgtcgg cgcgtttgtc gccgcgctcc
     7381 tgttcgaagc aggaaagaaa ggtttcgcgc tttatatcac catgttcccg tcatatcagc
     7441 tcatttacgg cgtgttggcg gtgatcccca ttctctttgt ttgggtctac tggacatggt
     7501 gtatcgtctt gcttggcgcg gaaattactg tcactctcgg ggaataccgc aaactaaaac
     7561 aagcagctga acaagaagac gacgacgaac catgattgca ttaattcaac gcgtaacccg
     7621 tgccagcgtc accgtggagg gagaagtgac gggcgaaatt ggcgcgggac ttttggtgtt
     7681 attgggtgtc gaaaaggatg acgacgaaca gaaagcaaac cgtctgtgcg agcgtgtgct
     7741 cggctaccgc atctttagcg atgccgaagg caagatgaat ctcaacgtgc aacaggcggg
     7801 cggcagtgta ctggtggttt cccagtttac cctcgccgca gataccgaac gggggatgcg
     7861 cccaagtttc tctaaaggcg catcaccgga tcgcgcagag gcgttatatg attatttcgt
     7921 cgaacgctgc cgccagcaag agatgaacac gcaaacagga cgcttcgctg ccgatatgca
     7981 ggtatcgctg gtcaatgatg gccccgtgac attctggttg caggtatgag ccagcttcca
     8041 gggttgtcac gggaaacaag agagagtatc gctatgtatc accttcgggt tccacaaaca
     8101 gaagaagagt tagagcgtta ctatcagttt cgctgggaaa tgttgcgtaa gcccctgcat
     8161 caaccaaaag gttcggaacg cgacgcgtgg gatgcgatgg cgcatcacca gatggtcgtc
     8221 gacgagcagg gtaatctggt ggcggtaggc cgattgtata ttaatgccga caatgaagcg
     8281 tccattcgct ttatggccgt tcacccggac gtgcaggaca aagggttagg aacgctgatg
     8341 gcgatgaccc tggagtcggt ggcgcgtcag gaaggcgtta agcgcgtgac ctgtagcgcc
     8401 cgtgaagacg cggtggagtt tttcgccaag ctggggtttg ttaatcaggg agaaatcacc
     8461 acgccaacca ccacgccgat tcgccatttt ttgatgatta agcccgtcgc cactctggat
     8521 gatattttgc atcgcggcga ctggtgcgcg cagctgcaac aggcgtggta cgaacatatc
     8581 ccgcttagtg aaaaaatggg cgtgcgtatt caacaatata ccgggcaaaa atttatcacc
     8641 accatgccgg aaaccggtaa tcagaatccg caccatacgc tgtttgccgg gagtttattc
     8701 tcactggcaa cgctcaccgg ttgggggctt atctggctga tgctgcgcga acgccatctc
     8761 ggcggaacga ttattctggc ggatgcgcat atccgctaca gcaagccgat tagcggtaaa
     8821 cctcatgcgg tagccgacct tggtgcctta agcggcgatc tcgaccgtct ggcgcgcgga
     8881 cgaaaagcac gggtgcagat gcaagttgaa atctttggcg acgagacgcc gggtgcagtg
     8941 tttgaaggca cgtatatcgt tctgcccgcg aagccatttg gcccgtatga agagggcggg
     9001 aacgaagaag agtaggggcg ttttacttga ctgaaaaggt tttcaatctg aaaacctttt
     9061 cagggcccag gtccgaagtt accttcccgc tctccaggca acatcccgta gtgccgatcg
     9121 cgcggcctca ctgataccac ctaactgaaa aaatccgtgg ataacgccaa ggtagcgttg
     9181 gcaggtgcat tgcactcctt gccctgtcat gcggtgatac aacgcttcac cttcatcgca
     9241 aagtgggtcg tattcagcgg taatgatatg tacaggtggc aagccattga aatcatcgcg
     9301 ccaaagaggg ctggcttcag gatgcctgcg ttcaatgcct ggcagataca tctcaaagcc
     9361 gctcagtaaa gtgtcgcgtg tgatgatgta atcgtgacca ttgcgtatgt agctttcgaa
     9421 atgcgcagtc gcatcaagca taggataaat taagatgagt tgtgccggtt gccactcgcc
     9481 agcacgcttt aatcgcaaag cagttaccag cgccagatgt ccgcccgcac tgtcaccggc
     9541 aagagttata cgttgtttat caacgccgag tcgttcggcg tgttgccaga ctatttctgc
     9601 acctcgttgc gcatcatcat gggcagcggg gaaagtatgt tccggtgcca gccgatactg
     9661 gacggcaata acccgacatt gcccgtaata ggctaactgg cgcagctggt tgtcgtgagt
     9721 tgcgaacccg ccgctaacaa aacagccacc gtagtaataa attatcgtcg ggagtaatgt
     9781 cggggcattc agcggcgaca ctattcgtaa ggtcatccct tcaagttcga tagtctgaat
     9841 atctacccgc gtttctgtct cacccgcaag gacagcgctg gcgatatagc cctccctgcg
     9901 ctgcgcaata ctttgttgac gcgatgaggg gcgacccgca gcgataaact tttcgactaa
     9961 ctctgcaatt cctttttcca gtgccatagt agaacttatt ttgctgtatg aatgtacagt
    10021 cttatacacc cgtttttgct ggatggatac agggaaattc acgggcgtga aaaatctgga
    10081 aagcgcctcg caaatctaaa gacaaacccg gcagagacgt ggttttgctg gagcactatc
    10141 agtaagctag tggtttcaag ggtacgtcaa tgacgtacaa aaaggattgt tactctaagc
    10201 caatggcgta taattatgct cttcatcgaa acggaaattt ttactgaaga tgttcaaaaa
    10261 ctgctgaccg acgatgaatt cagtcgtttt cagtttttcc tggccttgaa tcctgactat
    10321 ggtgaggtaa tacctgaaac tggtgggtta agaaaggttc gatgggtgtc aggaggaaag
    10381 gggaaacgtg ctggtgtcag ggttatttac tttcatcagg ttaaacatta tgagatcaga
    10441 ttactgctta tttatcgtaa gggtattaaa gatgatctgt ctccccagga aaaagcaatg
    10501 ctccggttgt taaatacgag gtggtaaatg gataaggcac tttttgagcg gttgactcac
    10561 agcatggccc agatgaatga aattattgaa ggaacgcgac agccttcgcg gacttttgaa
    10621 gttgatgcta tgaaaatcaa agaaatacgc cgcgcttcag ggttgtcaca atctaaattt
    10681 gccgatctta tttctgtaag cgtagacacg ttgcgtaact gggagcaggg gcgacgttca
    10741 ccgaccgggc ctgcaaaggc tttgctgcga gcgattgcca atgatccgca acatgtgctg
    10801 caagcactta accgttagag gcatggttat gccatacaga aagcactagc attcagaact
    10861 cctgtgaaag cggcatagtg gtcgcctgcc caggagtttt gctatgaccc gtaatcgtgc
    10921 tgaacgtcgc caccctatga tgcgattaaa aaaactcatc gtcgcgctga tgattgacgt
    10981 caatgctatc ggatgatacc ggggggttcc ggaaggaatt ccttgtttcc cgaactgtta
    11041 ccaacaaaat tataacgatg aacaagtaaa gaccggttgg tggcagtact gaccggtctg
    11101 tgatggatga ttaatttcac cttaaatccg ggtgctgtta ggatgctgac atttttcggg
    11161 tacaccattc agattatgca aataaagatc caatcaaaaa ataaacatcc ctgaatataa
    11221 tatattcata gcatccataa ctcatcctgg gcgataacta aaccattttt gagtaataaa
    11281 tgaaacaact gcaacactct tattatttct ggaaaacgcc tcgcaaaaac aaaacaacat
    11341 gcaaaaatcc gcttttttta aatgattttc cggcgtaatt ttcgcacata cattaatgag
    11401 taatataggt aaggtggtgg taatggcgat gaatacggtt tttcttcatt tatcagaaga
    11461 ggcaattaaa cggctgaaca aacttcgggg ctggcgtaag gtttcacgat ctgcaatttt
    11521 acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta aagcaaaggg
    11581 aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc ataaggaatg
    11641 aaaaaaattt ttcattcttg aatataaaaa aacagatgcc cttattctgg tattaataca
    11701 aggctgtttt acttgaactt ataataactg caactgttac atcgtatctg gaaaacgcct
    11761 cgcaaaaata aaaaatgatg cgtaaatgag ctttctttaa agattcggca agggtacttt
    11821 tctctccata gattcaaaaa tgatattggc gagatattta tgaactctct ggcaggtata
    11881 gatatgggca gaattttact caatttatcg aatgaggtga ttaagcaact tgatgatctc
    11941 gaggtgcagc gtaatcttcc tcgtgcggac ctattaaggg aagcggtaga tcaatacctg
    12001 ataaatcaat cgcaaacagc aagaaccagt gttcctggca tctggcaagg gtgtgaggaa
    12061 gatggtgtcg aatatcagcg taagctgcgc gaggaatggt aatgggaaaa aatgatgtca
    12121 atcagattgc tgacaacgtg cgcgttgttc
//
LOCUS       DAZFDP010000141        11953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFDP010000141 DAZFDP010000000
VERSION     DAZFDP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..653
                     /gene="yebB"
                     /locus_tag="ACHJ1G_003965"
     CDS             51..653
                     /gene="yebB"
                     /locus_tag="ACHJ1G_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO4765732.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(655..1176)
                     /gene="ruvC"
                     /locus_tag="ACHJ1G_003966"
     CDS             complement(655..1176)
                     /gene="ruvC"
                     /locus_tag="ACHJ1G_003966"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4765733.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1211..1951)
                     /gene="yebC"
                     /locus_tag="ACHJ1G_003967"
     CDS             complement(1211..1951)
                     /gene="yebC"
                     /locus_tag="ACHJ1G_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4765734.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1980..2432)
                     /gene="nudB"
                     /locus_tag="ACHJ1G_003968"
     CDS             complement(1980..2432)
                     /gene="nudB"
                     /locus_tag="ACHJ1G_003968"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4765735.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2550..4322)
                     /gene="aspS"
                     /locus_tag="ACHJ1G_003969"
     CDS             complement(2550..4322)
                     /gene="aspS"
                     /locus_tag="ACHJ1G_003969"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4765736.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4632..5198
                     /gene="yecD"
                     /locus_tag="ACHJ1G_003970"
     CDS             4632..5198
                     /gene="yecD"
                     /locus_tag="ACHJ1G_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4765737.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5195..6013
                     /gene="yecE"
                     /locus_tag="ACHJ1G_003971"
     CDS             5195..6013
                     /gene="yecE"
                     /locus_tag="ACHJ1G_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4765738.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6066..6461
                     /gene="yecN"
                     /locus_tag="ACHJ1G_003972"
     CDS             6066..6461
                     /gene="yecN"
                     /locus_tag="ACHJ1G_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4765739.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6502..7245
                     /gene="cmoA"
                     /locus_tag="ACHJ1G_003973"
     CDS             6502..7245
                     /gene="cmoA"
                     /locus_tag="ACHJ1G_003973"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4765740.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7242..8213
                     /gene="cmoB"
                     /locus_tag="ACHJ1G_003974"
     CDS             7242..8213
                     /gene="cmoB"
                     /locus_tag="ACHJ1G_003974"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4765741.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8378..10807)
                     /gene="torZ"
                     /locus_tag="ACHJ1G_003975"
     CDS             complement(8378..10807)
                     /gene="torZ"
                     /locus_tag="ACHJ1G_003975"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4765742.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10832..11932)
                     /gene="torY"
                     /locus_tag="ACHJ1G_003976"
     CDS             complement(10832..11932)
                     /gene="torY"
                     /locus_tag="ACHJ1G_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4765743.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2718 a         3189 c         2905 g         3141 t
ORIGIN      
        1 attttttatg ctattacgta tattcatatc tatttcaatg gaatgacaac gtgaatatta
       61 attaccctgc tgaatatgaa attggtgata tcgtctttac atgtataggt gctgccttat
      121 ttggtcaaat atcagctgca tcaaactgct ggagtaatca cgtcgggatc attatcggtc
      181 ataacggtga agactttctg gttgcagaaa gccgtgttcc cctctcaacc atcactacgc
      241 tatcccgttt tattaaacgc tctgctaatc aacgctatgc tataaagcga ttagacgccg
      301 ggctgacaga acaacagaaa caacgaattg ttgaacaggt tccctcccgg ctacgcaaac
      361 tttaccacac gggttttaaa tacgaatctt cccgccagtt ctgttcaaaa tttgtctttg
      421 atatttataa agaggcgcta tgtattccgg tgggtgaaat agagacgttt ggacaactgt
      481 taaatagcaa tccgaatgca aaactcactt tctggaaatt ctggttctta ggttctattc
      541 cgtgggagcg taaaaccgtc acgccagcca gtttgtggca tcatccgggt ttggtgttga
      601 ttcacgcgga gggagttgaa acgcctcagc ctgaactgac cgaggcggta taacttaacg
      661 cagtcgccct ctcgccaggt tcagccgcga ttcgctcatc tgcattgcat tctggctgac
      721 gtgacaatgg gtaatcgcga tagctaacgc atcagcggca tccgcttgtg gattagcggg
      781 cagtttcagc aaggtgcgga ccatatgctg cacctggctt ttctcggcgc taccaatacc
      841 taccaccgtt tgctttacct gacgtgccgc gtattcaaat accggcaatt cctgattcac
      901 cgccgccaca atcgccacgc cgcgcgcctg gcccagtttc agggctgagt cagcgttctt
      961 cgccataaag acttgttcaa tggcgaaata atcaggctgg aactgggtga tgatttccgt
     1021 cacgcccgca tagatgagct tcagacgaga cggtaaatca tccactttgg tgcgtatgca
     1081 tccactaccc aggtaggaca gttgcctgcc tacctggcgg atgacgccat agccggtcac
     1141 gcgcgaaccc ggatcaatgc cgagaataat agccatcacg cgtctccgtt ttgctgttta
     1201 gcaggcctca tcagagagtc gctgcaacct catcagagat ttcaccgtta tggtaaactt
     1261 cctgcacgtc gtcgcaatct tccagcatat cgatcagacg catcagtttc ggtgcggttt
     1321 ctgcatccat atcagctttg gtggacggga tcatggaaac ttccgcgctg tctgctttca
     1381 gacctgccgc ttccagagcg tcgcgcactt tacccatttc ttcccatgcg gtgtagacat
     1441 caatcgcgcc gtcatcatag gtcacaacgt cttcagcacc tgcttccaaa gctgcttcca
     1501 tgatggtgtc ttcatcgcct ttctcgaagg agatcacgcc ttttttgctg aacagatagg
     1561 ctacggaacc atcagtaccg aggttaccgc cacatttgct gaatgcatga cgcacttcag
     1621 caacggtacg gttgcggttg tcagacagac attcaatcat gattgccgtg ccgccaggac
     1681 cgtaaccttc gtagatgatg gtttccatgt ttgcatcatc atcaccgccc acaccacgtg
     1741 caattgcgcg gttcagtgtg tcgcgggtca tgttgttaga cagtgcttta tcaattgctg
     1801 cacgcagacg cgggttagcg tccggatcgc caccgcccag cttagccgcg gttaccagct
     1861 cacgaatgat tttagtgaag attttaccgc gctttgcatc ctgcgcagct ttacgatgtc
     1921 tggtgttggc ccatttacta tgacctgcca taaaaatatc tccagatagc cctgcctgtt
     1981 caggcagcgt taatgacaaa ctgttcaatc gcctgccggt tgctccagga cttagtgagc
     2041 gccgccgcag caggcgcatc aagccacttg taagccagat gttcagtgaa aacgatctgc
     2101 cgctcgtgcg gaagcgcaag acagaaccat gattccgtat tacgcgtcac gcccggcgca
     2161 tagcgatgac gtaaatgtga aaaaatttca aactctaccg tgcgctgaca gtcaattaag
     2221 gtcagttgtt cagcgacaac atcaatggtg acctcttcct ttacttcgcg catggcagct
     2281 tgcggcgcgg tttcaccctc ttccacgctg ccggttaccg actgccagaa atcgggatcg
     2341 tcacgccgct gcaacatcag cacccgtttc gtatcttgtg catagatgac cactaagatc
     2401 gaaacgggac gcttatacac tttatccttc acgctgcctc ttcgttgact gccttcgctc
     2461 atcccattca catagttatc tatgctcatg ggagttcact cagttgccgc cttgatgcaa
     2521 cgcgaatgat ttcgtgtatt tgagtcatat cagttattct cagccttctt cacaacctga
     2581 atgctcagct cagccagtgc agtcgggtta gcaaagctcg gtgcttcagt catcagacac
     2641 gctgccgccg tggttttcgg gaaggcgata acgtcacgga tattgtcggt gccagtcagc
     2701 agcatggtca gacggtcaag accgaatgcc agacctgcgt gcggcggagt accgtatttc
     2761 agggcgtcga gcaggaagcc gaatttctcg cgctgttcct cttcgttgat acccagaata
     2821 ccaaacaccg tctgctgcat atcaccatta tggatacgta ctgaaccacc gcccacttcg
     2881 taaccattga tgaccatatc gtaagcgttc gccaccgcat tttccggtgc agctttcagt
     2941 tctgcagcag tcatgtcttt cggtgaggtg aacggatggt gcattgccgt caggccgcct
     3001 tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca gcggtgccca tttgctttcg
     3061 tcggtcagac caaggtcttt acccactttc aggcgcagtg cgcccatcgc gtcggcaaca
     3121 attttcttgt tgtcggcacc gaagaaaatc atatcgccat cttgcgcggc agtacgatcc
     3181 aggatggctt cgatgatttc tgcattaagg aacttcgcta ccgggctgtt gataccttcc
     3241 agacctttcg cgcgttcgtt aactttgatg taagccagac ctttcgcgcc gtagatttta
     3301 acgaagttac cgtattcgtc gatctgctta cgggtcagcg atgcgccacc cggaacacgt
     3361 agagcggcaa cacggccttt cggatcgttc gccggccccg caaatactgc aaactcaaca
     3421 gatttcagca gatcggcaac gtcggtcagt tccatcgggt tacgcagatc cggtttatca
     3481 gaaccataac ggcgttctgc ttccgcaaag gtcattaccg ggaaatcgcc cagatccacg
     3541 cccttcactt ccagccacaa atgacgcacc agcgcttcca tcacttcacg cacttgcggc
     3601 gcggtcatga aagaagtttc cacatcgatc tgagtaaatt caggctgacg atcagcgcgc
     3661 aggtcttcgt cacggaagca tttaacgatc tgatagtaac ggtcaaaacc ggacatcatc
     3721 agcagctgtt tgaacaactg tggggattgc ggcagtgcgt agaatttacc tttgtgcaca
     3781 cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag catcggagtt
     3841 tcgatgtcga ggaagccgtg gtcatccata aaacggcgca ccaggctggt gattttagcg
     3901 cgggttttca ggcgctgagc catttccgga cgacgcaggt cgaggtagcg gtatttcaga
     3961 cgcgcttctt cggtgttgac gtggttagag tcaagcggca gaacatctgc gcggttgatg
     4021 atagtcagcg aggacgccag cacttcgatt tcgccggtcg ccatatcgcg gttaatattt
     4081 ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt acgcagttca
     4141 gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac gataccttcg
     4201 cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac gacgacggtt gacccaacca
     4261 cacagagtca cctgctgccc cacgtgggac aaacggagct gtccacaata ttctgtacgc
     4321 atgagatatc ccttaactta gctgccggcg gatgccccct gctgcgcagg tgaccaagtc
     4381 gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg gctattatac tggaaattct
     4441 gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt taccacttta tatcgggcct
     4501 gaaatcagac tctacgccag tttgctataa aggtgttgcc cgaactcata aaaattaaca
     4561 aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt gaggcacgat tttgtttttt
     4621 caggagtcat tatgcttgaa cttaatgcta aaaccactgc gctggtggtg atcgatttac
     4681 aagaaggcat cttacctttt gccggtggtc cacatactgc cgatgaggta gttaatcgcg
     4741 ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg gtgcgcgttg
     4801 gctggtctgc cgattacgcc gaagcattaa aacagccggt tgatgccccc tcccccgcta
     4861 aagtgttgcc cgaaaactgg tggcaacatc cagctgcatt aggtgcaact gacagtgata
     4921 tcgaaatcat caaacgtcag tggggtgcgt tttacggtac ggatctggag ttgcaactgc
     4981 gccgccgagg tatcgataca atagtgttat gtgggatctc gaccaatatc ggtgttgaat
     5041 ccaccgcccg caatgcctgg gaacttggct ttaatctggt gattgccgaa gacgcctgta
     5101 gtgccgctag cgccgagcag cacaataaca gcatcaacca tatctaccca cgcatcgccc
     5161 gtgtgcgtag cgtggaagag atccttcacg cgttatgatt tacatcggtc taccgcaatg
     5221 gtcgcatcct aaatgggtgc ggttggggat caccagcctt gaagagtatg cccgccactt
     5281 taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg
     5341 ctggcgtgaa cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc
     5401 gcatcaggca gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc
     5461 accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg
     5521 ggagctgcct gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt
     5581 ggaagtccgc catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg
     5641 tttacatcag cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcagcatg
     5701 tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc
     5761 tgtactgacg gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa
     5821 ccgggaatta tttcaggtct ggttacagaa attagcgcag tggcatcaga ccactacgcc
     5881 ttatcttttt ttacatacgc cagacattgc ccaggccccg gaactggtac ataccctgtg
     5941 ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc
     6001 ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag
     6061 tttgtatggt aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt
     6121 ttgatgtcgt tcgcttgcgt ttgcagtacc gcgttgccta tggtgacggt ggttttagcg
     6181 aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg
     6241 tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca
     6301 tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct
     6361 ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc
     6421 tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc
     6481 tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc
     6541 agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag
     6601 cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc
     6661 gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg
     6721 gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg
     6781 atgattgaac gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc
     6841 attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt
     6901 accctgcaat tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga
     6961 ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt
     7021 ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg
     7081 gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc
     7141 cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt
     7201 aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact
     7261 tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga
     7321 ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg
     7381 aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa
     7441 gcgaagagcc actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga
     7501 tgccatggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt
     7561 ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc
     7621 tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc
     7681 tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac
     7741 tactgggtaa cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac
     7801 tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg
     7861 agcatctctg gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc
     7921 tggttattga tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc
     7981 gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt
     8041 ttgtagatat ccgcgttgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg
     8101 aatggatggt taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag
     8161 ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg
     8221 gcattactgc cggatgcggc ttgaatgcct tatccggcct acaaaccctt gctaattcaa
     8281 tctattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc
     8341 ataacctcag cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcaggcttat
     8401 caaatgccgt taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt
     8461 taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac
     8521 tgccgttttt acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca
     8581 cgcctttttt gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc
     8641 agacacgcac cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag
     8701 tgatcggttc acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac
     8761 tatgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac
     8821 ccttccactc atcaggcgca agccaggttg gatgtgccgg gcaatcctta tagccaaatt
     8881 tttccagcgt tttggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca
     8941 ccggatcggc gcggaaatca gcataacgaa cgtactgttc gttcttctcg ctgcggcgca
     9001 tttcgatcag tttattttgc tgccagaagg cattaaacat cggcatagtc acgcgttgcg
     9061 cacgagcacc tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac
     9121 cttcggtata gatctctttt ccgccaggtt tcagtaattc agcaagatcg gcaaatacgt
     9181 caaaatcgtt acgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct
     9241 ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta
     9301 atacgatatc tgcatgttta gcagctgcgg tccagtagca ttcagaaacg acgatcatct
     9361 ccggtttctg ccatgctttg atcagacgat tagtatcctg gtgatgggta aagttaccgc
     9421 cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat
     9481 atttgccacc aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgtcattc
     9541 cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca
     9601 cgccgccaac acgcgtcgga ttgccaccgt tggagtaatg ataagagaaa ccaaaaccgc
     9661 cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct
     9721 gttcaccata ttgctggcgc tgaattcccc agccagccat cagcattgtg cgattcgccg
     9781 ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc
     9841 aggccgcgct cttcggagta ttatcgctct tacctgtcag atactcttca aactgcggat
     9901 acccggtggt gtatttttca aggaacactt tatcgtgttt accttgtgtc atcagggtat
     9961 gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat
    10021 tatcaccaaa gaactcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc
    10081 cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa
    10141 tttttaaggt attcagcggg ttcatccccc acagcaccac agcctggctg ttttccagaa
    10201 tcagcggcca ggaggtctgc tgttcataca cctctacaga accgaccaca tgcggcatga
    10261 tcacctgtgc tgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt
    10321 tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt
    10381 atgatccggc aaaaatagca gatggtccgt tagctttgcg aatgcgatcg tgttgctcat
    10441 gaatcagttt taatgcttgc tcccagctca cctgtacata agtatcttcg ccacgacctt
    10501 tcgccggttg cagtggatta tcgagatagc tttttcttac catcggatgc tgaatacgtg
    10561 ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg gttttcgcca
    10621 gcgcgcctgt tgaagaaaca atcttgccgt ccttcacttc tacgttcatc gctccccaac
    10681 gtcccgcggt gaggatttta ccgcccttct cttctgccca tgcggggagc ggtgctgccg
    10741 atgtcaccac cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta
    10801 atgtcataac ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt
    10861 tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgcgcac
    10921 ccattccttt ggcaatggac ggccatgcat tgacggtgta atggtcagcg gcaataggcg
    10981 catggcaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac
    11041 ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc
    11101 cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta
    11161 atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca
    11221 tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat
    11281 cggtaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat
    11341 gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat
    11401 ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc
    11461 cgcccttttg tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca
    11521 tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc
    11581 atactgtttc ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc
    11641 cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca
    11701 tccctgactt tggaatatgg caatctgcac attccgcacg tatccctttc tggttcgaaa
    11761 agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa
    11821 atgctgtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa
    11881 ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct
    11941 ctaaataaat tcc
//
LOCUS       DAZFDP010000142        11870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFDP010000142 DAZFDP010000000
VERSION     DAZFDP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(235..882)
                     /gene="ychE"
                     /locus_tag="ACHJ1G_003977"
     CDS             complement(235..882)
                     /gene="ychE"
                     /locus_tag="ACHJ1G_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4765744.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(913..966)
                     /gene="ychE"
                     /locus_tag="ACHJ1G_003978"
     CDS             complement(913..966)
                     /gene="ychE"
                     /locus_tag="ACHJ1G_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4765745.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1359..4034
                     /gene="adhE"
                     /locus_tag="ACHJ1G_003979"
                     /gene_synonym="adhC"
     CDS             1359..4034
                     /gene="adhE"
                     /locus_tag="ACHJ1G_003979"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4765746.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4158..5054
                     /locus_tag="ACHJ1G_003980"
                     /pseudo
     CDS             4158..5054
                     /locus_tag="ACHJ1G_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5336..5953)
                     /gene="tdk"
                     /locus_tag="ACHJ1G_003981"
     CDS             complement(5336..5953)
                     /gene="tdk"
                     /locus_tag="ACHJ1G_003981"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4765747.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6558..6971
                     /gene="hns"
                     /locus_tag="ACHJ1G_003982"
     CDS             6558..6971
                     /gene="hns"
                     /locus_tag="ACHJ1G_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4765748.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7115..8023)
                     /gene="galU"
                     /locus_tag="ACHJ1G_003983"
     CDS             complement(7115..8023)
                     /gene="galU"
                     /locus_tag="ACHJ1G_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4765749.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8225..9238)
                     /gene="rssB"
                     /locus_tag="ACHJ1G_003984"
     CDS             complement(8225..9238)
                     /gene="rssB"
                     /locus_tag="ACHJ1G_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4765750.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9330..10235)
                     /gene="rssA"
                     /locus_tag="ACHJ1G_003985"
     CDS             complement(9330..10235)
                     /gene="rssA"
                     /locus_tag="ACHJ1G_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4765751.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10348..10806
                     /gene="ychJ"
                     /locus_tag="ACHJ1G_003986"
     CDS             10348..10806
                     /gene="ychJ"
                     /locus_tag="ACHJ1G_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4765752.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10856..11698
                     /gene="purU"
                     /locus_tag="ACHJ1G_003987"
     CDS             10856..11698
                     /gene="purU"
                     /locus_tag="ACHJ1G_003987"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4765753.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3212 a         2940 c         2695 g         3023 t
ORIGIN      
        1 aaggcagtgg aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg
       61 tggcgaaaat aacatgatgg tgaggagaag cagttaagga aatccttata actaactgta
      121 atttgagaag aatattttta cctattcctt ttgcttttga taactgcgcg ggaacaaagt
      181 aaaaataatg acgaagtaaa cggagcatgg cagctccgtt tcatttgaaa ggaattaatt
      241 aagcaggccg gggaaaatcc ccttaatacc agtaacgata aattcaatcc ccaatgccat
      301 cagcaatagc cccataatac gcgtaatcac gttgatgccg gtctggcgta aaacccgtac
      361 cagccacggt gccatgcgga acaatcccca gcaacataaa gcgaacaatg caatagccac
      421 aaagaaacca aacagatagc taatgctgtg ataacgcgta ccccagacga tggtagaact
      481 gatcgcccct ggccccgcca tcaacggcaa cgccaatggc accacaccaa tgctttcacg
      541 taccgcggtt tctgattttt cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat
      601 catcgacatc gctatcgtca ccaccaggat gcccccggcg atacggaacg aatcaattga
      661 tataccaaaa agttgcagaa tcgtgtcgcc gagaaaaagc gagatccaca agataatggc
      721 cacagacagg ttggctgtaa ggttagtttt gtttcgcgct gccgctgtct gataactggt
      781 catgctgata aagacgggaa taatccctac cgggttgacc agcgcaaata acccgatgaa
      841 aaatttgaag taaacgggaa aatcaaaaaa ggtctgaatc acggttagct ccgaagcaaa
      901 agccggataa tgttagccat aaataaggtt gaaaagacgc gctgacaata cgccttttga
      961 cagcattttt cacctcctaa ctacttaaaa ttgctatcat tcgttattgt tatctagttg
     1021 tgcaaaacat gctaatgtag ccaccaaatc atactacaat ttattaactg ttagctataa
     1081 tggcgaaaag cgatgctgaa aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat
     1141 cggtacccag aagtgagtaa tcttgcttac gccacctgga agtgacgcat tagagataat
     1201 aactctaatg tttaaactct tttagtaaat cacagtgagt gtgagcgcga gtaagctttt
     1261 gattttcata ggttaagcaa atcatcaccg cactgactat actctcgtat tcgagcagat
     1321 gatttactaa aaaagtttaa cattatcagg agagcattat ggctgttact aatgtcgctg
     1381 aacttaacgc actcgtagag cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc
     1441 aagagcaagt agacaaaatc ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc
     1501 cactcgcgaa aatggccgtt gccgaatccg gcatgggtat cgtcgaagat aaagtgatca
     1561 aaaaccactt tgcttctgaa tatatctaca acgcctataa agatgaaaaa acctgtggtg
     1621 ttctgtctga agacgacact tttggtacca tcactatcgc tgaaccaatc ggtattattt
     1681 gcggtatcgt tccgaccact aacccgactt caactgctat cttcaaatcg ctgatcagtc
     1741 tgaagacccg taacgccatt atcttctccc cgcacccgcg tgcaaaagat gccaccaaca
     1801 aagcggctga tatcgttctg caggctgcta tcgctgccgg tgctccgaaa gatctgatcg
     1861 gctggatcga tcaaccttct gttgaactgt ctaacgcact gatgcaccac ccagacatca
     1921 acctgatcct cgcgactggt ggtccgggca tggttaaagc cgcatacagc tccggtaaac
     1981 cagctatcgg tgtaggcgcg ggcaacactc cagttgttat cgatgaaact gctgatatca
     2041 aacgtgcagt tgcatctgta ctgatgtcca aaaccttcga caacggcgta atctgtgctt
     2101 ctgaacagtc tgttgttgtt gttgactctg tttatgacgc tgtacgtgaa cgttttgcaa
     2161 cccacggcgg ctatctgttg cagggtaaag agctgaaagc tgttcaggat gttatcctga
     2221 aaaacggtgc gctgaacgcg gctatcgttg gtcagccagc ctataaaatt gctgaactgg
     2281 caggcttctc tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg
     2341 aaagcgaacc gttcgcacat gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag
     2401 atttcgaaga cgcggtagaa aaagcagaga aactggttgc tatgggcggt atcggtcata
     2461 cctcttgcct gtacactgac caggataacc aaccggctcg cgtttcttac ttcggtcaga
     2521 aaatgaaaac ggctcgtatc ctgattaaca ccccagcgtc tcagggtggt atcggtgacc
     2581 tgtataactt caaactcgca ccttccctga ctctgggttg tggttcttgg ggtggtaact
     2641 ccatctctga aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag
     2701 ctgaaaacat gttgtggcac aaacttccga aatctatcta cttccgccgt ggctccctgc
     2761 caatcgcgct ggatgaagtg attactgatg gccacaaacg tgcgctcatc gtgactgacc
     2821 gcttcctgtt caacaatggt tatgctgatc agatcacttc cgtactgaaa gcagcaggcg
     2881 ttgaaactga agtcttcttc gaagtagaag cggacccgac cctgagcatc gttcgtaaag
     2941 gtgcagaact ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt ggtggttccc
     3001 cgatggacgc cgcgaagatc atgtgggtta tgtacgaaca tccggaaact cacttcgaag
     3061 agctggcgct gcgctttatg gatatccgta aacgtatcta caagttcccg aaaatgggcg
     3121 tgaaagcgaa aatgatcgct gtcaccacca cttctggtac aggttctgaa gtcactccgt
     3181 ttgcggttgt aactgacgac gctactggtc agaaatatcc gctggcagac tatgcgctga
     3241 ctccggatat ggcgattgtc gacgccaacc tggttatgga catgccgaag tccctgtgtg
     3301 ctttcggtgg tctggacgca gtaactcacg ccatggaagc ttatgtttct gtactggcat
     3361 ctgagttctc tgatggtcag gctctgcagg cactgaaact gctgaaagaa tatctgccag
     3421 cgtcctacca cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga
     3481 ctatcgcggg tatcgcgttt gcgaacgcct tcctgggtgt atgtcactca atggcgcaca
     3541 aactgggttc ccagttccat attccgcacg gtctggcaaa cgccctgctg atttgtaacg
     3601 ttattcgcta caatgcgaac gacaacccga ccaagcagac tgcattcagc cagtatgacc
     3661 gtccgcaggc tcgccgtcgt tatgctgaaa ttgccgacca cttgggtctg agcgcaccgg
     3721 gcgaccgtac tgctgctaag atcgagaaac tgctggcatg gctggaaacg ctgaaggctg
     3781 aactgggtat tccgaaatct atccgtgaag ctggcgttca ggaagcagac ttcctggcga
     3841 acgtggataa actgtctgaa gatgcattcg atgaccagtg caccggcgct aacccgcgtt
     3901 acccgctgat ctccgagctg aaacagattc tgctggatac ctactacggt cgtgattatg
     3961 tagaaggtga aactgcagcg aagaaagaag ctgctccggc taaagctgag aaaaaagcga
     4021 aaaaatccgc ttaatcagta gcgctgtctg gcaacataaa cggccccttc tgggcaatgc
     4081 cgatcagtta aggattagtt gaccgatcct taaactgagg cactataacg gcttccacaa
     4141 cagggagccg ttttcttatg ccacttctta atgatctgct cgatttcagt gaccatccgc
     4201 ttatgcctcc gccctctgca caactatttg cagaacacct tcccaccgag tggatacaac
     4261 actgcctgac gctttctgct catgcgaccg ttcgccgccg tcgtttaccg ggggacatgg
     4321 ttatctggat ggtggtgcaa tgagccaatt accgatgttg ttcgccgtct gaacctgagc
     4381 gcggatggcg aagcggggat gaacctgctg gcccgcagcg ctgtcaccca ggcgcgtcag
     4441 cgcgtggggg ccgccccagt ggaatggctc ttccgccaga ccgcacagac aggggcgcgg
     4501 aacgttacct gaaggatgac tggcacggcc tgcaactttt tgccattgat ggcgcacagt
     4561 tcaggacacc tgataaaccc gagctgcgtg aatattatgg gtctgccaac acatccacta
     4621 agcggcagaa cgcctacccg gttatgcgtc tggtggcctt aatgaacctg ggaagtcaca
     4681 ttctgctgaa tgccgtgacc gcaccttacc ggcagagcga aactgtgctg gcccactcca
     4741 tgctcgccac catcccggat aactctatta cgttgtttga taaacttttt tacagcgaag
     4801 acctgctgct gacgctgaac cagaagggtt gtaaccgcca ctggctactg cctgcgtgga
     4861 agaatatcgc atcagaaatg atagagctgg gaaacacggc gtcaacaggg accatcccga
     4921 aacgactgga gcatcttcgg ggtgctctgg aagtggtgtt cataacaaaa cgcccccgac
     4981 catcaaggcc gaggtcggtt aagatatcaa aaacccgtta tcctgtgaaa catagtgccg
     5041 ctcctcttaa gtgaacggca ttgcccttct ggggccgtat tttttgttta cccaaagcaa
     5101 cttttccata aaccgacagc attagccttc atcatatttg cgacgatgta taacgcctaa
     5161 acacagggat attgtacttt acaggtcaca agtcaacgtc ggtgcttaag agccctgtga
     5221 ggcgtatagc ggcgttaaaa aactgccgag aagggtatat agcccggaag aagtgcgtaa
     5281 aacgaactga caggataaaa gtgccctgct caccctgtca gtaaagaaat tcttattaat
     5341 cgtggcgatg cctttcctga atagccgtta atgagccgac ttgtaacgcc tctttatagt
     5401 gtttacggca tacagaaacg tatcgttcat taccaccaat taccacctgc tcaccttcgt
     5461 tataaggtct gcctgcttga tcaagacgca gcaccatgct tgctttacgg ccacaaaaac
     5521 agatggtttt taattcaacc agtttgtcgg accatgccag taagtattgg ctgccaataa
     5581 ataattcacc tcgaaaatcg gtacgtaaac cataacaaag tacgggtata tcgagttgat
     5641 cgacaacctc cgataattca tatacttgtt gtctggttaa aaactggcat tcatcaacca
     5701 gtacgcaatg aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat aatgatgaat
     5761 tttggttaaa taattttgca ggcgatgaca aacctatacg cgaactgact ttcccggcac
     5821 caaagcgatc atcaatttct gccgtatata cgacagtgcg catgccgcgt tcctggtaat
     5881 tgtatgaaga ttgcaacaat gctgtagact tacccgcatt cattgcggaa tagtagaaat
     5941 atagctgtgc catcagccac aggccctcaa tgatatgaaa taaagttggc tggagtttat
     6001 cataattcgt cagtttttca gctcaacagt atgcacagaa gtgggtatat gcgttctccc
     6061 ttacgaagcc ttgcataatc cttctgagct atcattacaa ctgcctcgct tgttataagc
     6121 gggtaaatga ctgctggtaa ctattcacaa tctttaacct gttgcgcaag taatagccct
     6181 ctgttgacct ccaggagata gtgcaatact aagtccatgc tcttattgcg actgttctac
     6241 ttttcatcat tcgcttaata gggaattctc gtaaacacaa ctaatacaga agactgaaag
     6301 gtcgtcagcc tacgataatc tccccataaa atgtgacatg aatcaggaag ttttaacctc
     6361 acgtgctgcg aaatcatcgg tgtaaatagg gctatatgcc gcgtcttttc tggctaattt
     6421 tatgaaaaga tatttattgg cggcacaaaa taaagaacaa ttttgaattc cttacattcc
     6481 tggctattgc acaactgaat ttaaggctct attattacct caacaaacca ccccaatata
     6541 agtttgagat tactacaatg agcgaagcac ttaaaattct gaacaacatc cgtactcttc
     6601 gtgcgcaggc aagagaatgt acacttgaaa cgctggaaga aatgctggaa aaattagaag
     6661 ttgttgttaa cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt gaagagcgca
     6721 ctcgtaaact gcagcaatat cgcgaaatgc tgatcgctga cggtattgac ccgaacgaac
     6781 tgctgaatag ccttgcttcc gttaaatctg gcaccaaagc taaacgtgct cagcgtccgg
     6841 caaaatatag ctacgttgac gaaaacggcg aaactaaaac ctggactggc caaggccgta
     6901 ctccagctgt aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac gatttcctga
     6961 tcaagcaata atcttttgta gattgcactt gcttaaaatc ccgccagcgg cgggattttt
     7021 tattgtccgg tttaagacaa tttaataaga ataagctata aaaaaacggc gtcgattgct
     7081 caacgccgtt tcgtggataa caccgatacg gatgttactt cttaatgccc atctcttctt
     7141 caagccaggc tttaaattcc gtgccaaggg tgttatgacg aataccgtat tcaacgaagg
     7201 cctgcatgta acctaattta ttaccgcagt catggctctt ccctttcata tgataggctt
     7261 ccaccgtttc tttttcaatc agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag
     7321 ctcccggagg ggtttttgcc agcaacggcc aaatatccgc gctaagtacg taacgaccca
     7381 caatagcgag attagacggc gcaacatccg ctttcggttt ttctaccaca ccaaccatcg
     7441 gtacgctttc acccggcgct aattcaacgc ctttgcaatc cacaacgcca tatgcggtca
     7501 catcagcaac cggttcaacc atgatctggc tatgacccgt ttcatcaaag cggcggatca
     7561 tctctgccag gttatcctgt gacaaatcgg attcatattc atccagaata acatcaggca
     7621 aaataacagc taccggttca tcacccacta ccgggtgagc acacaatacc gcgtgtccca
     7681 ggcctttcgc cagaccctga cgaacttgca taatagtcac gtgcggtgga caaatagact
     7741 gcacttcatc aagcagttga cgttttacac gtttttccag cattgcttcc agttcaaaac
     7801 tggtatcaaa gtggttttca atagagtttt tagatgagtg tgtaaccagc acaatttcag
     7861 taatgccagc cgcaatacat tcattcacga cgtattgaat taatggctta tcgacaagtg
     7921 gcagcatctc tttcgggatg gctttcgtcg ccggcaacat cctggttcct aatcccgcaa
     7981 cggggataac ggcttttttg actttcgtat taatggcagc catttaaatt ctcctggact
     8041 gttcgtgttt tgaacgtgtt catatttctg tatcgcatcc cagtatatca gcaccccgaa
     8101 ggggctaagt tctgaaaagg aggcgagtta gcataattaa gacaattact tatacgaatg
     8161 gcgacgatag taccaccagc cgacattagc aggtaatgca aatttagccc gcgttatcgt
     8221 ttgctcattc tgcagacaac atcaagcgca gtcgaccacc ggttccccat atttggcatt
     8281 gccaggcatc gcatcgctgg ctcaattgat tcaaataagc gttacctaaa gtgcctaacg
     8341 gaacaccatt actgatttgc acctggtgtt cgccggtatt taacgtcgca ttcagacccg
     8401 cagaaaccag aatgagattt ttcagttcgc gatgataata gccaactaat agcggaaact
     8461 gccccggcag attggcctga cgaagtaaat ggtttacctg cttcaataac gcgcccaact
     8521 ctggcaaccg ttgattttgg tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc
     8581 gtaataacaa ggcagcaagt acgccattat gtccagctcg ggtgacatca aggcaataaa
     8641 atgccagatc gttttccgaa agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg
     8701 caaccaattg acgataatta acccggcaat gggaaatcac ctgctgaacc ggcggttgta
     8761 gttcctgtaa taatttcgcc gctgcggcag ggttatcaac cattgcatcc cagtcgcgaa
     8821 aaagcctttc ctcttcctca acgcgcgaat taaacatgct gggatagaga caggcaaaaa
     8881 ccatctcgcg caagcgattc aaatctttaa ctggtttcag caaaacatct tcaacgccca
     8941 gacgtaacgc tttggcaata tctgccatat tttcggtggc agatatcacc agaaccggtg
     9001 tctggtcacc tctgttgcgt atatgttcca gcagtttaag cccgttcatt cgtggcatcg
     9061 cgatatcaca tatcatcagg tctggagtga aacctcccag caactcaagg gcatccaccc
     9121 catcagccgc cagtaccgtt gtcgctccca atgaggaaaa ccacgaatcc agaagcgagc
     9181 gaaataccgg ctcatcttca acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt
     9241 tctctcccct gactggcttt actcaatagt ggcatgctat tgccacctgc gcctgtcaga
     9301 attaacttaa gtgtagataa aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt
     9361 tcgtccattt tcttttccac tgccagctgt ccggccgcaa tggcagcgtg cgcgcgatgg
     9421 aaatcaagcg tagatatttg cgggcaaaca ggttgaatca gaatatcggg cggatcacct
     9481 gccatgcggt tccttttaag gcggttctcc agcacctgga tagaagtggt cataatctct
     9541 gttgccgttg gcgctgtcac cgcccgacgt gtcgttatgc tgcctaacct ttctttcaga
     9601 cgcgcatgcc acggcagaga atcaccattc tcgctatttt cttcactgac attaaaggag
     9661 agcaaatctt gttgcatcaa atgagcatcg tgctgcaggt caaccgctat cacaatatca
     9721 gcccccaatg cacgcgtgag ggaaatagga attgggttaa cgactgctcc atcaaccagc
     9781 cagtagccgt tatgtgcaac aggtgccatg agtcctggaa tactgcatga tgcgcgaata
     9841 gcaagatgga gatcgccttc agtaaaccat aattcacgtc ccgtacttaa attggtggca
     9901 acagccgcaa agcgacggga acaattttcg atctctgttt ccggcattat ttcgcggtat
     9961 tgattgaaga cacgctcgcc gcgcagtaac ccaccgcgct gccaggagag atccatcagg
    10021 cgtaaaacat cccaataact gaaagaggtc acccaatctt ccagcgcaga taatcgatcg
    10081 catgcatagg cagcgcccac cagcgaacca attgaacatc ctgcaacgat atcaatttca
    10141 atacccactt tttttagcgc attaataacg ccaatatgcg accaacctct cgccgcgcca
    10201 gatcccagcg ccagccctat ttttatcttt ctcattattc ccgcaagatt cccctggaat
    10261 gcaatcgtag ccacatcgct actgctcggt taacatagtg ctactcaggc ggttaatacg
    10321 ccgttattgt ttccagggag attatttgtg tctcagcttt gtccctgtgg tagtgctgtc
    10381 gagtatagcc tatgttgcca cccttatgtg tctggtgaaa aggttgcacc tgatccagaa
    10441 catctcatgc gttcgcgtta ctgcgctttt gtgatgcaag acgcagatta tttaataaag
    10501 acctggcatc cctcttgtgg agcagcggcg ttacgtgccg aattgatggc cggatttgcg
    10561 catactgaat ggttgggatt gaccgtattt gaacattgtt ggcaggacgc cgacaatatc
    10621 ggatttgtca gttttgttgc tcgatttacc gaaggtggta aaacaggtgc aataattgaa
    10681 cgttcgcgat ttttgaaaga aaacggtcaa tggtactata ttgacggtac acgtccgcag
    10741 tttggtcgca acgatccctg cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc
    10801 caataatggt tgacggtacg gtttagcaaa cactctcaac aaggtttttc cagcaatgca
    10861 ttcactccaa cgtaaagttc tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg
    10921 tattaccaat atttgctaca agcacgagtt aaatatcgta cagaacaatg aatttgttga
    10981 tcaccgtacc gggcgctttt ttatgcgcac ggaactggaa gggattttta atgattccac
    11041 cctgctggcg gatctcgata gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc
    11101 cggtcgtcgc cggatagtga ttctggtcac taaagaagcg cattgccttg gcgatttgtt
    11161 gatgaaagcc aattacggcg gcctggatgt cgaaatcgcg gcagttattg gtaaccacga
    11221 tactttacgt tctctggttg agcgttttga tattccgttt gagctggtaa gccatgaagg
    11281 gttaagccgc aacgagcacg atcaaaagat ggcggacgcc attgatgctt atcaatctga
    11341 ctatgtggtg ctggcgaagt atatgcgggt attaacgccg gaatttgtgg cacgcttccc
    11401 gaataaaatc atcaatattc accattcatt cctgcctgcg tttatcggcg cacgtcctta
    11461 tcaccaggcc tatgaacgtg gtgtgaagat tattggcgca accgctcact atgtgaatga
    11521 caatctggac gaaggcccaa tcatcatgca ggacgttatt catgtcgatc atacctacac
    11581 agctgaagat atgatgcgcg caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct
    11641 atacaaagta ctggcacagc gcgtctttgt ttacggtaat cggacgatta ttctttaatc
    11701 gccagcaaaa ataactggtt acctttaatc cgttacggat gaaaattacg caaccagttc
    11761 atttttctca acgtaacact ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc
    11821 cgataaggga gcaggccagt aaaaagcatt accccgtggt ggggttcccg
//
LOCUS       DAZFDP010000143        11455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFDP010000143 DAZFDP010000000
VERSION     DAZFDP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            336..1724
                     /gene="narU"
                     /locus_tag="ACHJ1G_003988"
     CDS             336..1724
                     /gene="narU"
                     /locus_tag="ACHJ1G_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4765754.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1806..5546
                     /gene="narZ"
                     /locus_tag="ACHJ1G_003989"
     CDS             1806..5546
                     /gene="narZ"
                     /locus_tag="ACHJ1G_003989"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4765755.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5543..7087
                     /gene="narH"
                     /locus_tag="ACHJ1G_003990"
     CDS             5543..7087
                     /gene="narH"
                     /locus_tag="ACHJ1G_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4765756.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7087..7782
                     /gene="narW"
                     /locus_tag="ACHJ1G_003991"
     CDS             7087..7782
                     /gene="narW"
                     /locus_tag="ACHJ1G_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4765757.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7779..8459
                     /gene="narI"
                     /locus_tag="ACHJ1G_003992"
     CDS             7779..8459
                     /gene="narI"
                     /locus_tag="ACHJ1G_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4765758.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8538..9431
                     /locus_tag="ACHJ1G_003993"
     CDS             8538..9431
                     /locus_tag="ACHJ1G_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4765759.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9527..10372)
                     /gene="nhoA"
                     /locus_tag="ACHJ1G_003994"
     CDS             complement(9527..10372)
                     /gene="nhoA"
                     /locus_tag="ACHJ1G_003994"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO4765760.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10545..11114
                     /locus_tag="ACHJ1G_003995"
     CDS             10545..11114
                     /locus_tag="ACHJ1G_003995"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4765761.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11118..11345)
                     /gene="pptA"
                     /locus_tag="ACHJ1G_003996"
                     /pseudo
     CDS             complement(11118..11345)
                     /gene="pptA"
                     /locus_tag="ACHJ1G_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2702 a         2970 c         3024 g         2759 t
ORIGIN      
        1 tggccttttt agtgaataaa tcatcactaa ttctataagt gtctggtctt gttgataata
       61 tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga catacgttca
      121 aagattaacc cccgtttgaa ggttagcttg ttagttacat ttagtaacac atatctggct
      181 acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc aatcagcact
      241 tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct
      301 tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat gagaaaaata
      361 gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa aataaaggaa
      421 aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg
      481 tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta
      541 ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc
      601 cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt agtactgcaa
      661 tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg
      721 ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca
      781 tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg
      841 gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg
      901 tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt
      961 cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcat
     1021 ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct
     1081 tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta
     1141 tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc
     1201 tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta
     1261 tttccgataa gttcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt
     1321 tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt
     1381 acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga
     1441 tgatcgccgt catctttcgc cagataacca tttatcgggt aaagatgaaa ggcggtagtg
     1501 atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag
     1561 ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga
     1621 ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc
     1681 tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat
     1741 gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc
     1801 atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg
     1861 ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc
     1921 gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta
     1981 gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc
     2041 cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt
     2101 actcctggta tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga
     2161 ttgaactgtg gcgcgaagcc ctcaagcaac acagcgatcc ggtactggcg tgggcatcga
     2221 ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta
     2281 tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca
     2341 aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt
     2401 cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta agtttctacg
     2461 actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac
     2521 cggaatctgc cgactggtat aactccagct acatcatcgc ctgggggtct aacgtaccgc
     2581 agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca
     2641 tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga
     2701 aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc
     2761 atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga
     2821 tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg
     2881 catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag
     2941 ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa
     3001 aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc
     3061 tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa
     3121 atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa
     3181 acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg
     3241 cgaattacgg cctcgatcgc gggctggaag atgaaaacag tgcgaaagat tacgctgaaa
     3301 tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg
     3361 aaaccatcgc ccgtgaattt gccgataccg cccataaaac gcatgggcgc tcgatgatta
     3421 tcctcggcgc aggtgttaac cactggtatc acatggacat gaactaccgc gggatgatca
     3481 atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg
     3541 gccaggaaaa actgcgccca caaaccggct ggttgccgct ggcctttgcg ctcgactgga
     3601 accgaccacc acgccagatg aacagtacct cgtttttcta caatcattcc agccaatggc
     3661 gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca
     3721 gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgc
     3781 cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc
     3841 ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac
     3901 cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct
     3961 cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc
     4021 agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg
     4081 cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct
     4141 gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca
     4201 cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat
     4261 cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg
     4321 gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag
     4381 agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag
     4441 gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct
     4501 ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga
     4561 atacgcagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg
     4621 cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg
     4681 caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg
     4741 ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata
     4801 ttcaggcgca gccgcgtaaa attatctcca gccccacctg gtccggtctg gaaagcgatc
     4861 acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt
     4921 cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg
     4981 tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag ataccgccaa
     5041 acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact
     5101 caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga
     5161 tcagcgaaac agatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtattca
     5221 acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga
     5281 ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca
     5341 tgcgcggcgg cattcataac tcggtcaccc gcgtttgccc gaaaccaacg catatgattg
     5401 gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcgga tcgaaccgcg
     5461 atgagttcat catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg
     5521 atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa gtcggcatgg tgcttaacct
     5581 cgacaaatgt atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccgggcg
     5641 cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc
     5701 gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa
     5761 gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt
     5821 ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag
     5881 cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg acggcaaacg
     5941 gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg agtttgaaaa
     6001 acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa
     6061 caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc
     6121 gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca
     6181 ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt
     6241 caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg
     6301 ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct
     6361 ttacgacgcc gaccgcattg aggaagcggc gagcaccgag cgcgaagttg acctctatga
     6421 acgtcagtgc gaagtattcc tcgatccaca cgatccctca gtgatcgagg aagccctgaa
     6481 acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc
     6541 gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc caatggtctg
     6601 gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag
     6661 cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat
     6721 gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg
     6781 ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt
     6841 aggcctgagc gtcgcccagg tcgaagagat gtatcgttac ctcgccattg ccaactatga
     6901 agatcgtttt gtcatcccga cgagccatcg ggaaatggcg ggcgatgcct tcgcagaacg
     6961 caacggctgc ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct
     7021 gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg
     7081 cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagctgttg
     7141 tgggaatgca aggaggacgc gctggcgttg atccgcagcg acgcgccgat gcttacggat
     7201 ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg
     7261 tttgaccgtg gacgtaccac atcgctgctg ctgttcgaac atgttcatgc cgagtcccgc
     7321 gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg
     7381 gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct
     7441 gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt
     7501 cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg
     7561 ggaagctccc tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac
     7621 acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac
     7681 gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc
     7741 gcgccgcaat atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct
     7801 ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc
     7861 gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc gataaacgcg
     7921 agatggtgat atggtcgaat ctgttccata tcggcatttt ggggattttc ttcgggcact
     7981 tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac
     8041 aactaatggc gatggtgctc ggcggtattt gcggtgtgtt gacattgatt ggcggcgcag
     8101 gactgctgtg gcgcaggctg acaaatcagc gcgtgcgtgc aacttccact acgccggata
     8161 ttatcatcat gagcattttg ctgatccagt gtctgctggg gctaagtacc ataccgtttt
     8221 cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg
     8281 tcactttccg tggtggatcg tcagaaatgc ttaacggcgt agcgttcgtt ttccgcctgc
     8341 atctggtatt gggaatgacc atcttcctgc tcttcccgtt caccagattg gtgcatgtgt
     8401 ggagcgcgcc gtttgagtac tttactcgtc ggtatcagat tgtgcgttcg cggcgataat
     8461 ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca
     8521 gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctctcaac
     8581 catttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa
     8641 tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg
     8701 acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg
     8761 cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct
     8821 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
     8881 gtatttcgct ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg
     8941 aggcgattat caacgcgctt catctcactg aagacgatat tttgccgggc ttgccaattc
     9001 aggtcgcaac aacaggacac tcaaaagtga tgatcccgtt gaaaccggaa gtggatatcg
     9061 acgccctttc gcctgatctt gctgcgctga gcgctatcag caaacagatt ggctgcaatg
     9121 gtttcttccc gttccagatc cgcccaggca aaaacgaaac cgatggtcgt atgttctcgc
     9181 ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
     9241 ggttggtaca tcacaacgta ttgccccacg atggcaacgt gttatgtgtt aaaggccatc
     9301 aggggcgcgc attggggcgt gacggcatga ttgaagtgac ggtgacaatt cgtgataacc
     9361 aaccggaaaa agtcaccatt tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa
     9421 ttgaactctg aaaccgttgc ctgatgcgct tcgcctgtca ggtctacaac cgagctaatg
     9481 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
     9541 ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc atccacggta aagccatgtt
     9601 tcgcatcatc cacgcccaga ccaaactgtt cttgcatcac agcatataat gacgccacat
     9661 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
     9721 tcagcgttag cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
     9781 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
     9841 cgcagagatc aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc
     9901 aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg cgtggtctgc acgagatcgg
     9961 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
    10021 tttcctcttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    10081 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc
    10141 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
    10201 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    10261 acggaatagt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
    10321 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
    10381 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    10441 gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc
    10501 gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc
    10561 ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc atgatcacca
    10621 gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtca atgccggtgg
    10681 agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga
    10741 ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt
    10801 gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg
    10861 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg
    10921 agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggtgagg
    10981 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg
    11041 ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttaccagc ggcaagcgag
    11101 ttacggcggg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg
    11161 ggcgcgattt cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca
    11221 atgcttatcg agctatcttt actgttcaga tggcgaataa taacgtcggt aatatctgca
    11281 gcaagtgctg ctttttattg ttcgtccagt tcacgcggaa aacatttaat gtcgatgtgc
    11341 ggcatgattg tgctcctttt gaaagatgtc agtaaaatta acacacattg tgaatgtcgt
    11401 gtgtccggta atgcgttttt acgacaataa tgccagggca agattacgaa agcct
//
LOCUS       DAZFDP010000144        11376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFDP010000144 DAZFDP010000000
VERSION     DAZFDP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..829
                     /gene="ydeO"
                     /locus_tag="ACHJ1G_003997"
     CDS             68..829
                     /gene="ydeO"
                     /locus_tag="ACHJ1G_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4765762.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1231..2913
                     /gene="ydeN"
                     /locus_tag="ACHJ1G_003998"
     CDS             1231..2913
                     /gene="ydeN"
                     /locus_tag="ACHJ1G_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4765763.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKQKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2965..4122
                     /gene="ydeM"
                     /locus_tag="ACHJ1G_003999"
     CDS             2965..4122
                     /gene="ydeM"
                     /locus_tag="ACHJ1G_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4765764.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4088..4210)
                     /locus_tag="ACHJ1G_004000"
     CDS             complement(4088..4210)
                     /locus_tag="ACHJ1G_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765765.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            4413..6098
                     /gene="yddA"
                     /locus_tag="ACHJ1G_004001"
     CDS             4413..6098
                     /gene="yddA"
                     /locus_tag="ACHJ1G_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4765766.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            6136..8508
                     /gene="yddB"
                     /locus_tag="ACHJ1G_004002"
     CDS             6136..8508
                     /gene="yddB"
                     /locus_tag="ACHJ1G_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4765767.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8565..11348
                     /gene="pqqL"
                     /locus_tag="ACHJ1G_004003"
     CDS             8565..11348
                     /gene="pqqL"
                     /locus_tag="ACHJ1G_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4765768.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3362 a         2472 c         2463 g         3079 t
ORIGIN      
        1 cagcaaatta taaatatgaa atgttaaaaa agtatcgata aaaactttat tgttttaagg
       61 agataaaatg tcgctcgttt gttctgttat atttattcat catgccttca acgctaacat
      121 tttagataaa gattacgcct tctctgacgg cgagatcctg atggtagata acgctgttcg
      181 tacgcatttt gaaccttatg agcggcattt taaagagatc ggatttactg aaaataccat
      241 taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt
      301 tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc
      361 ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt
      421 tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg tttccggaaa
      481 agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga
      541 ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt
      601 ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg
      661 ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc
      721 gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag
      781 tcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa
      841 cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc
      901 gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc
      961 acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg tcagtaaaat
     1021 gcaataattt attaaatatc aataaattag ttgtttatcg gcgagaaatt acttaataga
     1081 acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc taaatctgct
     1141 atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa aattacttcg
     1201 gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta
     1261 agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca
     1321 gatgatgtaa agcagaaagc aaccaaaaca aacgttgctt tctcagactt tacgccgact
     1381 gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga tcttggttat
     1441 ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa tcgtgaagtt
     1501 gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa atcaacgccg
     1561 acgctccttt cattaatgga tgaaggcgta cggtttacta acggctatgt ggcacacggt
     1621 gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg ctttggtgtc
     1681 tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt gcctgaatta
     1741 ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc aaaaatcagt
     1801 aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt caccacattt
     1861 tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg attccacgct
     1921 gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg tgtccccgca
     1981 aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga tcgtgccaaa
     2041 acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct gccaaatgat
     2101 aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac
     2161 tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga acaactgaag
     2221 aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg tgcggttatc
     2281 gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc
     2341 ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac
     2401 aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga tatcagcatt
     2461 ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga taagaaacaa
     2521 ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt tgacgaggaa
     2581 aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga cgattacccg
     2641 cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa taacgattat
     2701 tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac ggatctacag
     2761 caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta
     2821 agagagttta tcgacagcag ccagccaccg cttagcgaag taaatcagga gaagtttaac
     2881 aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggaattttcc
     2941 gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt
     3001 aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa
     3061 tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc gtctggcaat
     3121 caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt
     3181 aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa tgcattacaa
     3241 acgaatggca ttttattgaa taatgaatgg tgtgcttttc tcaaagaaca tgagtttctg
     3301 gttggtatct cgatcgatgg cccccaggag ctacatgacc gttacagacg cagtaattca
     3361 ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaagtt
     3421 gagtttaata cgttaaccgt cattaataac gttaatgtcc attaccctct tgaggtttat
     3481 cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct cgaaaccggg
     3541 acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat tgatttttct
     3601 gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac
     3661 gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt tttggggaat
     3721 gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat
     3781 ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat
     3841 aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca
     3901 gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc taagcatcgt
     3961 attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa aaaccttttt
     4021 tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag agtaccgctt
     4081 tatcacatta tggatgttgc aaaacaaatg gagaataatt aataacacgc ccttttagct
     4141 ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt cctgtcgggt
     4201 gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg cgtcagggca
     4261 ttagcaatac ccttgattat caatagcata accataacta atgaaaatga aatatttctt
     4321 tatatttcat attgttaagt gatacttaaa taaatttcat cagtaaatca ttgccataga
     4381 taatcattgg atggcacaat caattttcgc taatgataac cattcccatt acgttttgta
     4441 tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag aataataaaa
     4501 cttcagtgtt gttaatcatt attatcctcg ccatgatcct tggcgtggtt aaaattcagg
     4561 tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa gaaaccgata
     4621 agctttggca gcttgtcctt tggttccctg ccctgctggg gatctttgta ttaatatccg
     4681 taaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg ttaacagatt
     4741 actaccttaa tcggtggttt gcagataaaa actactactt cacgcagatc tacggcgagc
     4801 ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta ctgatcagca
     4861 aaacgctcag cctttcattt ggcttcatcc agtcacttag catgctgatc acctttaccg
     4921 ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca gaatggaata
     4981 tccaggggta tatggtctat accgttgtgc tcattgtgat cggtggaact ttatttaccc
     5041 ataaggttgg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc gaagcgacat
     5101 ttcgaacaaa tcttgtgcag cataataagc aggcagaact gattgcgcta agcaatgctg
     5161 agtctctaca acgccaggag ttgagcgaca attttcatac cattaaagag aactggcatc
     5221 gtttaatgaa tcgtcagcgg tggcttgatt actggcagaa tatttattcg cgctcgctta
     5281 gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc aatctgggcg
     5341 gactgatgaa atcgcgccag gcatttatgc tggtatcgaa caatttaagc tggtttattt
     5401 ataaatatga cgaacttgct gaactggctg cggttatcga tcgtttgtat gagttccatc
     5461 aactcaccga acagcgccct acgaataaac ctaaaaattg ccaacatgcg gtacaagtgg
     5521 ctgacgcgag tattcgtact cctgataata agatcatatt agagaacctg aactttcatg
     5581 tttcgccagg caaatggcta ttactgaaag gttactctgg tgcgggaaaa accacactgc
     5641 ttaaaacatt atctcactgc tggccgtggt ttaaaggtga tatttcttct cctgctgaca
     5701 gttggtatgt gtcacaaaca ccgttaatca aaaccggctt actgaaagag attatttgta
     5761 aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat caggttggtc
     5821 tggggaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt ctttccagcg
     5881 gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa tggatatttc
     5941 ttgacgaaac tacctctcat cttgaggaac aagaggctat ccgcttactg cgtttagtgc
     6001 gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaatccggt gtctggaacc
     6061 tggcagatga tatttgtgac attagcgcgg ttttataaaa acaataaaag ccctgctgta
     6121 cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg tggcgctgat
     6181 gtggcgcagg ccgtcgatga caaaaacatg tacatgcatg tttttgaaga gatgacggtc
     6241 tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga aagcatcgag
     6301 cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc tgcggtacgc
     6361 atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgcccgga gaaaatctct
     6421 attcacggtg cgtcgcccta tcagaatgcc tatttgattg acggtattag tgccactaat
     6481 aacttgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag cgggatgtca
     6541 caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga cagtgttgtg
     6601 ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa acgcttcaac
     6661 gctgatgata gcaaggtgaa attgggttat cgcactacgc gttcggactg gttaacatcg
     6721 catatcgatg agaataacaa gagcgcattt aatcaaggct cttcaggaag tacttattac
     6781 tccccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggaact cgctgataac
     6841 ttcggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc ggattatgtt
     6901 tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga tactgcattg
     6961 agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt aaaatatacc
     7021 ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga aatgggtaat
     7081 aaatcctacg gtctggcatg ggatatggat acgcagctcg catgggccaa actgcgtacc
     7141 accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat ctggtacacc
     7201 gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg attaggacac
     7261 atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca aaaattcgcc
     7321 gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc cgatgcatgg
     7381 actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa gaaaactaac
     7441 cataccattt accataaagg taaaggcagc ctgggaattg acaactacac gctgtatatg
     7501 gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta tgactatgac
     7561 aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga tatttttgct
     7621 gatcaaacct caatgattac agcaggttat aaccgttact atggcgggaa tattcttgat
     7681 atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggcaa taaaaccctg
     7741 acgcgttatc aggatttgaa aacgccttat aacgatgaac tggcaatggg attgcagcag
     7801 aaaatcggta agaacgttat tgcgcgcgca aactatgttt accgtgaagc gcatgatcaa
     7861 atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga atataacaac
     7921 gacggcaaaa caaaaacgca ttcgttcaac ctcagttttg aactggccga acccctgcat
     7981 atcagccagg tagatattaa cccgcaaatt gtctttagct atatcaagag caagggcaac
     8041 ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt ggtttataac
     8101 ggtaatctgg tttcttacga tagcgttcca gtggcagatt ttaataaccc attaaagatc
     8161 tccttaaaca tggatttcac gcatcaaccg agcgggttag tgtgggcgaa tacgctggcc
     8221 tggcaagaag cgcgtaaagc tcgcattatc ctgggtaaga caaatgcgca atacatcagc
     8281 gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct gacctgggac
     8341 acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat cagtgccgat
     8401 attctcaatg tattggatag caaaaccgct gttgatacaa cgaataccgg tgtggcgacc
     8461 tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata gttcggattt
     8521 tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga aacctctgtt
     8581 tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc gccgccttac
     8641 cgcaggatga aaagttaatt accgggcaac tgggcaatgg cttgcgatat atgatttatc
     8701 cgcatgctca tccaaaagat caggtaaatt tatggctgca aattcatacc ggttcattgc
     8761 aggaagaaga caatgagcgc ggcgtggctc attttgtaga acatatgatg tttaacggca
     8821 caaaaacatg gccgggtaat aaagtcatcg aaacatttga gtcaatgggc ctgcgttttg
     8881 gtcgcgatgt taatgcctat accagctatg acgaaacggt gtatcaggtg agtttgccga
     8941 ctacgcagaa acaaaatctg caacaagtga tggcaatctt cagtgaatgg agtaatgccg
     9001 caacctttga aaaactcgaa gtagacgctg aacgtggcgt aattactgag gaatggcgtg
     9061 cccatcagga tgcgaaatgg cgcacctctc aggcgcgccg ccctttcctg ctggctaata
     9121 cccgtaattt agaccgtgaa cctatcggcc tgatggatac tgtcgccacg gtcacaccgg
     9181 cacaattgcg ccaattttat caacgctggt atcaaccaaa taatatgacc tttatcgtgg
     9241 ttggcgatat cgacagtaaa gaagcgctgg cgctgataaa ggataattta agtaagcttc
     9301 cggctaacaa agcagctgaa aatcgcgtct ggccgacaaa agccgaaaac cacctgcgct
     9361 ttaatatcat taatgataaa gaaaaccggg tgaacggcat cgcactctat tatcgcctgc
     9421 caatggtaca agtgcacgat gagcaaagct ttatcgaaca agctgaatgg agcatgttag
     9481 ttcagctgtt caatcaacgt ctgcaggaac gcatacagtc gggcgagttg aagactattt
     9541 ctggcggcac tgcgcgcagc gttaaaattg cacccgatta tcagtcgctg tttttccgtg
     9601 taaatgcacg agacgataat atgcaggatg ctgcgaatgc attaatggca gagttggcaa
     9661 ctattgatca gcatggtttt tctgctgaag aactcgatga tgttaaatct acccgcctca
     9721 cctggctgaa aaatgcggtt gatcagcaag ctgaacgtga tttacgtatg ctgaccagtc
     9781 gcctggcatc cagctcatta aataatacgc cgttcttgtc gccggaagag acatatcaac
     9841 tttcgaaacg tctgtggcag caaattaccg tgcaaagtct ggcggaaaaa tggcagcagt
     9901 taagaaagaa ccaggacgca ttttgggagc aaatggtaaa caatgagctt gccgccaaaa
     9961 aagcattgtc tcctgcggct atcctggcgc tggaaaaaga gtacgccaac aaaaagctgg
    10021 cggcttacat cttcccaggc agaaatttat cgttaacagt agacgctgac ccacaggcgg
    10081 aaattagcag caaagaaacg ctggctgaga atctgacatc attaacactt tccaatggtg
    10141 ccagggttat tctggcaaaa tccgcgggtg aagagcaaaa gctacaaatt actgccgtat
    10201 cgaataaagg cgatttaagt ttccctgcgc agcaaaaatc acttatcgcg ctggcaaata
    10261 aagcagtaag cggaagcggc gttggtgagc tctcctcttc cagcctgaaa cgctggagtg
    10321 cggaaaattc ggtaaccatg agcagtaaag tcagtggcat gaatacgttg ctctccgtta
    10381 gcgcgcggac taataaccct gaacctggtt ttcagttgat taaccagcga atcacccaca
    10441 gcacgattaa cgacaatatt tgggcatcgc tacaaaatgc tcaaattcag gcgttgaaaa
    10501 cgctcgacca gcgtccagcg gagaaattcg cccagcagat gtatgagacg cgctatgctg
    10561 atgaccgcac gaaattactg caagaaaatc agattgtaca gtttactgcc gcagatgcgc
    10621 tggctgccga tcgccagttg ttttcatctc cagcggatat cacgtttgtc attgtcggta
    10681 atgtctcaga agacaaactc gtggcgttaa ttacgcgtta cttaggatca atcaaacact
    10741 ctgattcgcc attagccgca ggtaaaccat taactcgcgc gacggacaac gcatcggtta
    10801 ctgtaaaaga acaaaatgaa cctgtggcac aggtttcaca gtggaagcgt tatgattccc
    10861 ggacacctgt taatctggcg acgcgtatgg cgctcgatgc ttttaacgtc gcactggcaa
    10921 aagatctacg tgttaatatt cgtgaacagg catctggagc atacagcgtt tcttctcgcc
    10981 tctcggttga tcctcaggcc aaagatatca gtcatttgct ggcttttact tgtcaaccag
    11041 aacgacatga tgaactgtta acgttagcga atgaagtgat ggttaagcgt ctggctaaag
    11101 ggatcagtga gcaagaactg aatgaatacc agcaaaacgt tcagcgcagc ctcgatatcc
    11161 aacagcgtag cgttcaacaa ttagcgaaca ctattgtaaa tagtcttatt caatatgacg
    11221 atcctgcagc atggactgag caggagcaat tgttgaaaca aatgacggta gagaatgtta
    11281 acactgccgt taaacaatat ctttctcatc cggtaaatac ttataccgga gtattattgc
    11341 caaaataata acagccccgt caacacatcg ttggcg
//
LOCUS       DAZFDP010000145        11196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFDP010000145 DAZFDP010000000
VERSION     DAZFDP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..145
                     /locus_tag="ACHJ1G_004004"
     CDS             11..145
                     /locus_tag="ACHJ1G_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4765769.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            124..228
                     /gene="speFL"
                     /locus_tag="ACHJ1G_004005"
     CDS             124..228
                     /gene="speFL"
                     /locus_tag="ACHJ1G_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4765770.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            539..2737
                     /gene="speF"
                     /locus_tag="ACHJ1G_004006"
     CDS             539..2737
                     /gene="speF"
                     /locus_tag="ACHJ1G_004006"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4765771.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2734..4053
                     /gene="potE"
                     /locus_tag="ACHJ1G_004007"
     CDS             2734..4053
                     /gene="potE"
                     /locus_tag="ACHJ1G_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4765772.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4109..5749)
                     /gene="pgm"
                     /locus_tag="ACHJ1G_004008"
     CDS             complement(4109..5749)
                     /gene="pgm"
                     /locus_tag="ACHJ1G_004008"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4765773.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5775..6320)
                     /gene="seqA"
                     /locus_tag="ACHJ1G_004009"
     CDS             complement(5775..6320)
                     /gene="seqA"
                     /locus_tag="ACHJ1G_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4765774.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6505..7269
                     /gene="ybfF"
                     /locus_tag="ACHJ1G_004010"
     CDS             6505..7269
                     /gene="ybfF"
                     /locus_tag="ACHJ1G_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4765775.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7409..7702
                     /gene="ybfE"
                     /locus_tag="ACHJ1G_004011"
     CDS             7409..7702
                     /gene="ybfE"
                     /locus_tag="ACHJ1G_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4765776.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7842..8372
                     /gene="fldA"
                     /locus_tag="ACHJ1G_004012"
     CDS             7842..8372
                     /gene="fldA"
                     /locus_tag="ACHJ1G_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4765777.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8582..8668
                     /gene="uof"
                     /locus_tag="ACHJ1G_004013"
     CDS             8582..8668
                     /gene="uof"
                     /locus_tag="ACHJ1G_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4765778.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8661..9107
                     /gene="fur"
                     /locus_tag="ACHJ1G_004014"
     CDS             8661..9107
                     /gene="fur"
                     /locus_tag="ACHJ1G_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4765779.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9191..9517)
                     /gene="chiQ"
                     /locus_tag="ACHJ1G_004015"
     CDS             complement(9191..9517)
                     /gene="chiQ"
                     /locus_tag="ACHJ1G_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4765780.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9567..10973)
                     /gene="chiP"
                     /locus_tag="ACHJ1G_004016"
     CDS             complement(9567..10973)
                     /gene="chiP"
                     /locus_tag="ACHJ1G_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO4765781.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2706 a         2758 c         2796 g         2936 t
ORIGIN      
        1 ttcataatta atgatttatt atttggggta tgatcgtttt ttgttgatct tcttcacaga
       61 ttatagccat ttcatggata gaataactct accttcaact gacacagcaa gaggtaaagg
      121 taaatggaaa ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag
      181 tttgcccatc gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa
      241 agggcacccc aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc
      301 ctcggtgctc atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg
      361 caacttgagg tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca
      421 aaaagcaaac tactgattta tttatcagcg gtggagcttt gctttttttc cggcgtgatc
      481 gatttctcct ttgagaaatt gaggacctgc tattacctga aataaagaga tgaacaaaat
      541 gtcagaatta aaaattgccg ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc
      601 atgcgtgaat atagacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcag
      661 tgatgttgaa cgtggaaaac tcgatgaaat agacgctact ggctatgaca ttcctgtttt
      721 tattgcaacg gaaaatgaag aacgtgtacc cgcagaatat ctgccacgta tttctggtgt
      781 cttcgaacat tgcgaatcac gtaaagagtt ttatggacgt cagttagaaa ccgctgccag
      841 ccattatgaa acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgttaatca
      901 gggcaatagt gcttttgact gccctggtca tcaggggggt gagtttttcc gtcgccatcc
      961 ggcaggcaat cagttcgtgg aatactttgg cgaaatgctc ttccgctctg acttgtgtaa
     1021 tgctgacgta gcaatgggcg atctgctgat tcatgaaggt gcgccatgta ttgcacaaca
     1081 gcatgcggct aaagtcttta atgccgataa aacctatttc gttttaaacg gtacttcatc
     1141 ttctaataaa gtggtcttaa acgcattgct gacgccgggc gatctggtgt tgtttgaccg
     1201 caataaccat aaatctaacc accacggtgc gttgctacag gctggcgcga cacctgttta
     1261 tctggaaacc gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga
     1321 agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg
     1381 tcctttccgc ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg
     1441 ccaggtggtg gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt
     1501 tggctatgag caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa
     1561 tgagaacgat ccgggtattc tggttacgca atccgtacat aaacagcagg ctggtttttc
     1621 tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc
     1681 gcacaaacgc atgaacaacg cctttatgat gcatgcctcc accagcccgt tctatccgct
     1741 gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg
     1801 gatggattgt gtggtaaatg gcattaatgc ccgcaagctg atcctcgata actgtcagca
     1861 tattcgtccg ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc
     1921 gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggtgagcact ggcactcttt
     1981 tgaaggctat gcagagaatc aatactttgt cgatccatgc aaactgttgc tgacaacccc
     2041 aggtattgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc
     2101 taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt
     2161 cctgctgact ccggcagaag atatggccaa gcttcagcaa cttgttgccc tgctggtacg
     2221 cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa
     2281 acagcatgaa gagcgctacg ccggttatac cctgcgtcag ttgtgtcaag aaatgcatga
     2341 tttgtatgcc cgtcacaacg tgaaacaact gcaaaaagag atgttccgta aggaacactt
     2401 cccacgcgta agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga
     2461 gctggttcgt ctgccggatg cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc
     2521 tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt
     2581 cagcgctctg gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt
     2641 ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga
     2701 tgcgcaaagc accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg
     2761 ggcgtcgttc agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg
     2821 ctgccgacaa agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc
     2881 gtaggctcaa tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca
     2941 ggcggtatgg gcggttatgc ggagtatgct ttcggtaaat ccggtaactt tatggcgaac
     3001 tatacctacg gcgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt
     3061 tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc
     3121 ggggtgctgt ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc
     3181 agtagcatta ccgtgtgggg ggtcattatt ccggtggtag gcctgtgcat tatcggctgg
     3241 ttctggttta gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc
     3301 agtgcagtag gttcttccat cgctatgacg ctgtgggctt ttctcggtct ggagtctgcg
     3361 tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc
     3421 ggtacgttag gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg
     3481 cctaatatgg agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttc
     3541 acaccagaag tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg
     3601 cttggctggc agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc
     3661 cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt
     3721 gtgattatcc agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc
     3781 aacgtgctgg ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg
     3841 gcattagtca ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac
     3901 tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa
     3961 gccatgctgt acggttccat cgtgaccttc cttggttgga cactgtatgg tctggtatca
     4021 ccacgctttg aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt
     4081 gcgaccgccc tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct
     4141 caaccgcttc tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga
     4201 tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca
     4261 ctttcagacc gccgatagaa gcaccattac ccggtgccgc cgtcagacgt gcggtgatcg
     4321 ggtcacctgc cagggtgctg gcgctcacca tttccggaga cagcttagac aacgccgctt
     4381 tttgtgcgga agtcgcagcg gcctgcaaac ggttgtagct cggcgcacca aagcgttttg
     4441 ccagttcgtt gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca
     4501 gcagacacat aatgatgccg tctttatcgg tggaccacgg cgtgccgtcg aaacgcagga
     4561 aggaagcccc ggcgctctct tcgccgccaa agccgaaact gccgtcgaac agaccatcaa
     4621 caaaccattt gaatcctacc gggacttcta ccagtttacg gcccaaggcg ttgaccacac
     4681 ggtcgatcat cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg
     4741 gacgatgctg gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac
     4801 ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga
     4861 acttatcacg cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga
     4921 tcgtgccgtc tttatcaagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga
     4981 tggtcaggtt gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc
     5041 ccagcggatc aacgccaagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat
     5101 cggccagacc ttccacaaac ggctgcacca gatcctgctc tttcacatga ccggatgcca
     5161 tcgcttcgtc gagagagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg
     5221 ccctgtcttc caccactttg gtgacgttgg tatcagccgg gccaccattt ggcgggttgt
     5281 acttgatacc accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca
     5341 gcgggccacc ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggagtga
     5401 atccattgtt ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg
     5461 aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga
     5521 tgccattttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt
     5581 taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct
     5641 ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa
     5701 tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct
     5761 ttgtctgcaa cgttttagat agttccgcga accttctcaa tcaattccgc cgggaattgc
     5821 atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc
     5881 acccaatacg gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc
     5941 gtttgttcat ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg
     6001 gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc
     6061 gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg
     6121 tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct
     6181 ttcgtcaccg gagcagcagg ctgtgatgag gcggaaaatt tcaacatacg ccgtaaaatg
     6241 tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca
     6301 tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca
     6361 ggagtatatg aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg
     6421 gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga
     6481 caacgcgaaa aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac
     6541 cagcacaata attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt
     6601 ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac
     6661 cacggtcttt caccgagaga tccggtaatg aattacccgg cgatggcgca ggatcttgtt
     6721 gatactctgg atgcacagca gatcgacaaa gcaacattta tcggtcactc catgggcggt
     6781 aaagcggtaa tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc
     6841 gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg agattttcgc ggctatcaac
     6901 gcggtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat
     6961 cttaatgaag aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc
     7021 tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg
     7081 gcatgggatc accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag
     7141 taccgtgatg atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg
     7201 ggtcactggg tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc
     7261 aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt
     7321 ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct
     7381 gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt
     7441 agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga
     7501 tgaacagctg cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa
     7561 gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggagtc
     7621 gcgcaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct
     7681 gcgtagtcag ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc
     7741 gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt
     7801 tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc actggcatct
     7861 ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg
     7921 gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt
     7981 atgacattct gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg
     8041 atgacttctt cccgactctc gaagagattg atttcaacgg caaactggtt gcgctgtttg
     8101 gttgtggtga ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg
     8161 acatcattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt
     8221 tcgaagcatc aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag
     8281 accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag
     8341 agttgcatct cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg
     8401 ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg
     8461 tacctgtaca atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac
     8521 gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat
     8581 aatgatacgc attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt
     8641 agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga
     8701 aagtaacgct tccacgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg
     8761 tcagtgcgga agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta
     8821 cggtatatcg cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg
     8881 aaggcggtaa atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc
     8941 tcgactgcgg caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa
     9001 ttgccgcaaa acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg
     9061 ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg
     9121 agaaaagcca acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta
     9181 cagctctcac ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt
     9241 tcaccgcctg atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc
     9301 gtacactttc ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca
     9361 cgctctggca ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata caggcgctgt
     9421 atgcctcttt cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc
     9481 ccgatgccat tatggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc
     9541 aggcacaaaa cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat
     9601 ttcacgtcac gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtca
     9661 gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc
     9721 tgaatggtgt agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg
     9781 tagtacgcat ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag
     9841 gaagcgccga tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag
     9901 aagaccgctt tttcgccgtt ggcgttgaag tcggaacggt tatcccacca gatatccagg
     9961 cggccgttgg aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg
    10021 tcagccttaa cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca
    10081 aaggtcaacg cttgcagcca ggcggtgccg tcataaaggt cgttgacgct gcgatcgtca
    10141 actttatcgc gcgtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca
    10201 aatttgtagc tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc
    10261 gcttccagta cgaagttatt tttgaagtcg tatttcgcac cgatggagtg caggtaatca
    10321 actttggtgg ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg
    10381 tattcgttgg tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc
    10441 gccccggctt ccgccccctg ataagtacct ggcataaagc tccagtgcgg cgctaacagc
    10501 gtttgaccag ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg
    10561 gccgcagctt tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct
    10621 ttattacttt ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt
    10681 tcaatcgccg taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga
    10741 aaatcgaggt tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg
    10801 gtaacatctt tacggtcgcg ttcacgctgc cagtaatata taccgccggt taaggttgaa
    10861 tcgtcgatga atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta
    10921 acaccggcga tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa
    10981 tcctctttga cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata aaaattaaaa
    11041 aagtagcgtt gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat
    11101 tatcaatctc tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga
    11161 tttttgttac aaggcttaag tttgcggttg aaaggc
//
LOCUS       DAZFDP010000146        11190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFDP010000146 DAZFDP010000000
VERSION     DAZFDP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..583
                     /locus_tag="ACHJ1G_004017"
     CDS             98..583
                     /locus_tag="ACHJ1G_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4765782.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            599..1075
                     /gene="radC"
                     /locus_tag="ACHJ1G_004018"
     CDS             599..1075
                     /gene="radC"
                     /locus_tag="ACHJ1G_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4765783.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHJ1G_004019"
     CDS             1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHJ1G_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4765784.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1528..1896
                     /locus_tag="ACHJ1G_004020"
     CDS             1528..1896
                     /locus_tag="ACHJ1G_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4765785.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            1986..2363
                     /locus_tag="ACHJ1G_004021"
     CDS             1986..2363
                     /locus_tag="ACHJ1G_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4765786.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            2360..2509
                     /locus_tag="ACHJ1G_004022"
                     /pseudo
     CDS             2360..2509
                     /locus_tag="ACHJ1G_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2609..2689
                     /locus_tag="ACHJ1G_004023"
                     /pseudo
     CDS             2609..2689
                     /locus_tag="ACHJ1G_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3505..3876
                     /locus_tag="ACHJ1G_004024"
     CDS             3505..3876
                     /locus_tag="ACHJ1G_004024"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO4765787.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
     gene            4147..4512
                     /locus_tag="ACHJ1G_004025"
     CDS             4147..4512
                     /locus_tag="ACHJ1G_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO4765788.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHJ1G_004026"
     CDS             complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHJ1G_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4765789.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHJ1G_004027"
     CDS             complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHJ1G_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4765790.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRHLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHJ1G_004028"
     CDS             complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHJ1G_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4765791.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6961..8127)
                     /gene="dacD"
                     /locus_tag="ACHJ1G_004029"
     CDS             complement(6961..8127)
                     /gene="dacD"
                     /locus_tag="ACHJ1G_004029"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4765792.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8336..9763
                     /gene="sbcB"
                     /locus_tag="ACHJ1G_004030"
     CDS             8336..9763
                     /gene="sbcB"
                     /locus_tag="ACHJ1G_004030"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4765793.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9806..10033)
                     /gene="tsuB"
                     /locus_tag="ACHJ1G_004031"
     CDS             complement(9806..10033)
                     /gene="tsuB"
                     /locus_tag="ACHJ1G_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO4765794.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10047..11105)
                     /gene="tsuA"
                     /locus_tag="ACHJ1G_004032"
     CDS             complement(10047..11105)
                     /gene="tsuA"
                     /locus_tag="ACHJ1G_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO4765795.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         2967 a         2894 c         2637 g         2692 t
ORIGIN      
        1 attgtgggtg atggcggaaa ccctgctgga gagcctgcgc tgattcagcc attaccctga
       61 aaaccacgca tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc
      121 caccacatct tctgtttccg taaccgctgc atcagggaat gaccagcctc agttggttgc
      181 caccatagtc cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc
      241 acagtgggtg accctggagc cccgtgtttt cggctggatg gaccgtctgt gcgaagacta
      301 ctgcggtggt atctggaatc tgtacactct gaacaacggc ggggcattta tggcccccga
      361 accggatgac gatgatgacg aaacatgggt actgttcaat gccatgaacg gcaaccgcgc
      421 tgaaatgagc ccggaagccg ctggcattgc tgcctgtctg atgacgtaca gtcatcatgc
      481 ctgtcgtacg gagtgttatg ccatgacggt ccattattac cggctgcggg attacgccct
      541 gcagcatccg gaatgcagcg ccattatgcg catcatcgac tgaatggagg cagggacaat
      601 gcagcagctt tcctttctgc ccggagaaat gacgcccggc gagcgcagtc tcattctgcg
      661 ggctctgaaa accctggacc gccatcttca tgaacccggt gtggccttca cctccacccg
      721 tgcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt
      781 gctgtatctg aataaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat
      841 caaccgcacg gaagtccatc cccgggaagt gattaaacgt gccctgtatc acaatgccgc
      901 agccgtggtg ctggcacaca atcacccgtc cggtgaagtc acccccagta aggcagaccg
      961 ccttatcacg gaacgtctgg tacaggcgct ggccctggtt gatatccggg tgccggacca
     1021 tctgatagtc ggtggcagcc aggttttctc ctttgcggaa cacggtctgc tttaacccgt
     1081 caccgtcaca accacatcac acctgttttc acttttatct tctgtcttca gaggtatcac
     1141 actatgaaaa tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc
     1201 cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagtgctga agcgtatacc
     1261 ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgcagaacg ccgtaaggac
     1321 agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg
     1381 aaaacgagta cgactggcag agctcagacg ccgctgtgga tgaggtcctg ctcatcaacg
     1441 aatacacctt taccgaagcc ggtctgcgcg ccggctgacc cgtcatcgtt aaactatcat
     1501 tcaccgattt tctgtgagga ttttatcgtg tcagacacac tttccgggac aacgcatccc
     1561 gatgacaatg acgaccatcc ctggtgggga ctaccctgca cagtgacgcc ctgtttcggc
     1621 gcccgtctgg tgcaggaggg taaccggttg cattaccttg ccgaccgtgc cggtatcaga
     1681 ggccggttca gagatgcgga tgcgtaccct ctggaccagg cctttccgct gctgatgaaa
     1741 caactgaaat taatgctcac cagtggtgaa ctgaatcccc gccatcagca taccgtgaca
     1801 ctgtatgcga aggggctgac ctgtgaagcc gacaccctcg gctcctgtgg ttacgtttat
     1861 ctggctgttt atccgacacc tgaaacgaaa aagtaaacca ccggaataac cctctactcc
     1921 ggcctggtgt tttcaccacg ccactgtccc attttttatc tctgcatatc aggaaaatct
     1981 tcagtatgaa aacattaccc gacacgcatg tacgggaggt atcgtgctgc ccgtctctcg
     2041 tcaccatctg gcagacactg ctcacccgac tgctggacca gcactacggc cttacgctga
     2101 atgacacacc gttcgctgat gaacgtgtga ttgaacagca tattgaggca ggcatttcac
     2161 tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct ggtacgtacc gaccagtcgg
     2221 gattcagcgc ctgtacccac tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     2281 gggcaacagg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggga
     2341 agcatccgga ggcgaaacga tgaaactggc cctgacgctg gaagccgaca gcgttaacgt
     2401 gcaggcgctg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcgctgaact
     2461 gattaacaag gtcgctgaaa acggtgattc actccgcgtg gtggatgaac gtgactctac
     2521 cgaaacaccg gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgataca
     2581 gggtatagtg ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga
     2641 agatgtcctt accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa
     2701 caggcttcat ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga
     2761 aaggaaaacc gataataatg gaatggacgc ccctaccatg aagcaacggt aggatattac
     2821 tgataaacct tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca
     2881 aaaaatgaat tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg
     2941 gattaaacgt tccctgatgc ccgtattctt cgcgaatatg tcttcctgtc ggattgggat
     3001 ggaggtctgc gccggagcgc gtcattagtg agaatgctgt ctacttaggg gcataatgtc
     3061 agactaatgc ccgcgatttg taaagccata tgtgaaaaca aacaagaatg atatggctgc
     3121 tgccgaggcc atctgtgaag ccgttacccg acctaatatg cggtttgtca gcatcaagac
     3181 gcctgagcag tagtcattac tgtcttttca tcgtgcccaa acgggatttg ttaaatcaag
     3241 ggctgcgcag ataaaccaga tcagagaatt actggctgaa tttggcatcg ttttcccgcc
     3301 cgacattgta gcattcagtc gtcatgtccc tgccctgcct gaagacgcgg aaaactgttt
     3361 aacaatgccg ttcagacagc tgtcgtccta tctctaatga aaatgtgaaa caactgagcg
     3421 tacacattga aaccatagaa gccgtgagca acgaacacgt cgggaacgac gaactgtgta
     3481 ggaaaattgc caccatttcg gggattgcta ttcttaccac cactgcaatt gtcagcaccg
     3541 ggtagtatcg gcttcaaaaa ccggcgtcag cttgctgcat ggcttggact ggttccccga
     3601 cagtgcttca gcgtaggtaa aaacacgctg gggtctatca gtaaacggga cgatgtctat
     3661 atcagaacgc tactgttcca tggtgcgaag acagtgtcaa acgccagaaa gggcagacag
     3721 gtccaggaaa gttggctggg cagaccagtc tcgcggcgga ataaaaatgt tgccaccgta
     3781 gcgctgaaca acaaaaatgc cagagtgctc tgggtattgc taaccaacga taaagagatt
     3841 tcaccagaag aagtgatggt agctgcatat atgtaaatga aactacttct gtctgttgaa
     3901 aaataaaacc aacctactga ttgctaagga taaacaagta ttaaaggcca gactgataag
     3961 accgcgacta agtgaacctg tatctgacat ggcatagctt cgctaatgtg tcatcatgat
     4021 taggactaaa tctgtgaatt cctttaggaa gcacggacac aggccaatct gagtccatat
     4081 gtatgattgg aatcttacac agatgacttt ttgacacggg gacttggcaa acaggggcga
     4141 ccatgtatgt cagatatgac aatctcactt ccgaaagaag tcaacgggca cgttatctgt
     4201 tcaaccagaa tccgacacat tctggctata cgaaagagca tacttatgca aatctatatg
     4261 cttttccggc aacaagcagg aatctcagcg ctgaggcagg aattagaggt aaaaacgccc
     4321 taccgggcca tgaatcaccc ggtcattgga gtagtaacca aagcagattt agccagcatg
     4381 gaacacatat ctttggtgaa atgttggtta cgggaagctg gagcgcacaa cgtgttagta
     4441 accagtgcgg ttaataacaa tggagttaca gaactttttg ccttgctgca tacagaagaa
     4501 ggctgttgct aatttcgata ggttctcaga tgagaatgat gacggtgata cacagataag
     4561 aatcaatgag caatgagggt tgccgggcaa ccctcattga ataaaacagg aattacttcg
     4621 cttcgccgtt tttcatttcg cccatagctt tcagcttttt ggagatatcg cggcgttctt
     4681 tggagagctc ggcatttttg atgatgtaat cgtcaacgcg atcttcatag tcacctttca
     4741 tgctggcgat gatgccctgg attgcttcaa cgctcatccc gggcttgatg taatcgctca
     4801 ggttgtccag caacaggacg cgcttctggt tgtcacggat ctttttctca acgtcctgaa
     4861 tttcacgttg cagtttgttc ttacgacgga acagacgaac aaattccagt acgtcctgga
     4921 atgaaggctt ggtagtttcc atttttatac ccctgataat gtgagagtcg gattcgttta
     4981 agcaaccgct attcgttagg gccaacatta ctcatagctg ccgcagatga caatgctttt
     5041 atcccttttc actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc
     5101 tgaagttcag gaattatttg cgcaaggccc ggcaaatcag actcgatagc aactcaagct
     5161 gatgtgccgt ttccatgttc agccacacat aaccatagat tggtgtttcc acaaccttca
     5221 ggtcatggtg gttattcagc aactgacgca gctcttccac agcatcgatc aatttttccg
     5281 tattggcaaa aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta
     5341 tttgctgcat catgtgctgg gtatcacgaa gtttttgcgc gttcaataac acgaaatggc
     5401 tggggcgcgt ggcccagtat gcattgattt gcaactccag catacaaacc agatggcggt
     5461 taatggtctg gataccttca tatatcgatt ttggaatacg agtttctttg ctggcgggcg
     5521 caatcagccc acgcattttc acggcatcgg tcaggagttt ttgtagatgg ctttccagac
     5581 gtgggcgttc gagtaagttc ggtgagaatg cagattgata aacccgatta tactcggtca
     5641 gacttttcgc cagttgaatg cgccagtgga tgaacgcccg ttgtggccag ataccggtaa
     5701 acaacattgc cagtaaagag ccgaggatca catcgccgct tcgccataac gccgtatcaa
     5761 tttcacctgt cggggaaccc acaacaattg ccaacgtcac cccaatcaat aatccttgat
     5821 acggtttctt gcccagcgcc agccaaccgc aaagaaacat agccgccgcg caccagacta
     5881 acatcagcgg taacgaaatt aactccagtt gcagagcgat aagacctaaa atcgaaccca
     5941 acaccgtacc gccaatacgt tcgaaggcgc gagggacaac gttaccccag aacgagattg
     6001 gccccataat caccaccatg gtgaccagcg gccaggtgct ttccgggata gtaaacaggc
     6061 ggataatgag aaaagtgagc aggaatgcca gcgcgacccg agtaccatgc acaatgcggt
     6121 aatggcggta tacccggatt tcaaacgggc ttaatgactt atcggcacgc acgcagaaac
     6181 tccgtaatga agattaaaag tgctcataac tcatctgtcg gccagaaggc ccgcctctga
     6241 attatccgac ggacaaaccg ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa
     6301 ggcgcatctt actcttttcg ctttcaaaaa aagagtggtc atcgcgttaa cacaccgccc
     6361 tgagatgaat tagtgatgtt ttggctgcac cgcaacatac atttcgatat cccagtaccc
     6421 atcttccgcg ccattgttca aataaacctc gaagcagggc tttggtaaca tttcataagc
     6481 actgtcctgc aagaggctat taaagaactg ataccagggt ttagcaaaat catcaccgac
     6541 tacacgagcc accgcaaccg catactgacc acctgaaatt tctgtcagaa tgacgccctc
     6601 actgttttcg gggagcgtaa agttattcgg caccgtcacg acgatgtcgc agcgtaattt
     6661 ttcggcgggt gtttcatctg gattgtcgta aaagacagca acccactcct tcggcacaat
     6721 atttttgcta tctacccaca tcatcaactg ctcaaagcct ttctttaccg tctgttccca
     6781 cgggccaacg agatggaaac ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt
     6841 catgacgtcc cattaacact gtttttatat acagtataat tgcgatatta aaaccacaag
     6901 gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc aaaagaaagg
     6961 tcaggcctta tggtggaaat aatcactcag gcgagaaaac atgctgcctt ccccgacaga
     7021 ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa gttcaatttc
     7081 ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa gggtatattt
     7141 ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt gttccgttcc
     7201 caggtcgata ttttctttat cgccatacca gatgcgttcc gtaccgacct ttttcccacg
     7261 gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt ttcttgcctc
     7321 ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac gacgctgccc
     7381 atcgacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg ttttcaggcc
     7441 gtcaacattc atggttttat cccacaataa cccgttacgg ttttgctggg tgataccgtt
     7501 ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgtcgt ggatgatagc
     7561 gcgagaaagc acagctaaat cataagccga gctatgctga cctggtgcat ccagaccatg
     7621 cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt tcatcatttc
     7681 aacaaactgc cgttgcccac cggcaatata gtcagccagt gcaacacaag cgtcatttcc
     7741 ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc cctctttcaa
     7801 aaacatcagt gaagaaccga caaacactgg attatctttc gcccacgcat cgcgccccac
     7861 ggtgacaata tcgtctggcg taatgcgatg actatcgata gtgcgatcca cgacatagcc
     7921 cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac ccgcggtgag
     7981 gatctgaccg gtggtgtaat ccatcagtac ccaggagccg gcatgaatct ctggaggctg
     8041 aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa tgaacaaaga
     8101 agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa atagctgttc
     8161 tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt gactaccctc
     8221 tcatttttat ctgacatgat ctgttgtcac tcgctgtcaa attgtcgcgc taaagctgat
     8281 tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta acctaatgat
     8341 gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct ttggcacgca
     8401 ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgatgacg aattcaatgt
     8461 catcggcgaa cccgaagtct tttactgcaa gcccgcggat gactatttac cccagcctgg
     8521 agcagtatta attaccggta ttaccccgca ggagacacga gcgaaaggag aaaacgaagc
     8581 cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta
     8641 caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga
     8701 tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttactgg atgttatgcg
     8761 tgcctgttat gccctgcgcc cggaaggaat aaattggcct gaaaatgatg acggtctacc
     8821 gagctttcgc cttgagcatt taaccaaagc gaatggtatt gaacatagca acgcccacga
     8881 tgcgatggct gatgtgtacg ccactattgc gatggcaaaa ctggtaaaaa cgcgtcagcc
     8941 acgcctgttt gattatctct ttacccatcg taataaacac aaactaatgg cgttgattga
     9001 tgttcctcag atgaaacccc tggttcacgt ttccggaatg tttggtgcat ggcgcggcaa
     9061 tacaagctgg gtggcaccgc tggcgtggca tcctgaaaat cgcaatgccg taattatggt
     9121 ggatttggca ggagacattt cgccattact ggagctggat agcgacacat tgcgcgagcg
     9181 tttatatacc gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtaca
     9241 tatcaataaa tgtccggtac tggcccaggc gaatacgcta cgcccggaag atgccgaccg
     9301 actgggaatt aatcgtcagc attgcctcga taacctaaaa attctgcgtg aaaatccgca
     9361 agtgcgtgaa aaagtggtag cgatattcgt ggaagccgaa ccgtttacac cttcagataa
     9421 tgtggatgca cagctttata acggcttttt cagtgacgcc gatcgtgcag caatgaaaat
     9481 tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg ttgataaacg
     9541 aattgaaaag ctgttgttca attatcgggc gcgcaacttc ccggggacgc tggattatgc
     9601 cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgccagagt ttttacaagg
     9661 ctatgcagaa gagattcaga tgctggcgca gcagtatgcc gacgataaag aaaaagtggc
     9721 gctgttaaaa gcgctttggc agtacgcgga agagattgtc taatgtgaag cctccgccgc
     9781 tggtacggcg gaggacaatt ttgagtcagg ctttttgaac ggtgatgctc cacgctgcat
     9841 cgccaatctg ctgataatcg gtgatggcat gtccttcttc tgccgcccac tgtgggattg
     9901 cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg gccatttctg
     9961 ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc acatccagct
    10021 ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag tcgtgcctta
    10081 cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat cataaataca
    10141 aggccaatcc agccctgcca ggtcatcatt gcagtcatga ccagtccatt accgatagag
    10201 caaccacctg caatgctggc accgaagccc atcagtacac cacctaaccc gctacgtaat
    10261 gttgtttgtg catcagcagc gcgaacgcgg aactcacggc tcgctttggc tgcaataaat
    10321 gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt cacgtcaccc
    10381 gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc aaacatgcgt
    10441 ccggttgctt cactcagggg ccaagctaaa agcgcaatca aaccgatgag tacagcagtg
    10501 acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccggttcg gcgcggcggt
    10561 aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca gagcgttatc
    10621 accagcaaaa cggcaaccaa cggccacaca gacaaattaa aagtatcagc aatagagtta
    10681 tgttcagtag tgtagtgctg caaggtttga tttaaaccac tggcgtgtgg agaacgcatc
    10741 accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa tccctcaccc
    10801 gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag cccgaaaaga
    10861 tacccaccta taacagtacc aagccacggg aacgccccgg cttcgtaagt cagtaggccc
    10921 gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag caaggcgtaa
    10981 aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag gcaaaaacgc
    11041 ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa tatcattgaa
    11101 aacataaatt aattaaccag atgaatgtta atggggaaat tattcatgac tggtggaatg
    11161 cagaccaata accaaattct ctaaattaga
//
LOCUS       DAZFDP010000147        11154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFDP010000147 DAZFDP010000000
VERSION     DAZFDP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..886)
                     /gene="dcuR"
                     /locus_tag="ACHJ1G_004033"
     CDS             complement(167..886)
                     /gene="dcuR"
                     /locus_tag="ACHJ1G_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4765796.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(883..2514)
                     /locus_tag="ACHJ1G_004034"
     CDS             complement(883..2514)
                     /locus_tag="ACHJ1G_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4765797.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2695..2925
                     /gene="yjdI"
                     /locus_tag="ACHJ1G_004035"
     CDS             2695..2925
                     /gene="yjdI"
                     /locus_tag="ACHJ1G_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4765798.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2937..3209
                     /locus_tag="ACHJ1G_004036"
     CDS             2937..3209
                     /locus_tag="ACHJ1G_004036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4765799.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3436..3732
                     /gene="ghoS"
                     /locus_tag="ACHJ1G_004037"
     CDS             3436..3732
                     /gene="ghoS"
                     /locus_tag="ACHJ1G_004037"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4765800.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3760..3933
                     /gene="ghoT"
                     /locus_tag="ACHJ1G_004038"
     CDS             3760..3933
                     /gene="ghoT"
                     /locus_tag="ACHJ1G_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4765801.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4052..5569)
                     /gene="lysS"
                     /locus_tag="ACHJ1G_004039"
     CDS             complement(4052..5569)
                     /gene="lysS"
                     /locus_tag="ACHJ1G_004039"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4765802.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5806..7263)
                     /gene="dtpC"
                     /locus_tag="ACHJ1G_004040"
     CDS             complement(5806..7263)
                     /gene="dtpC"
                     /locus_tag="ACHJ1G_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4765803.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7322..9469)
                     /gene="cadA"
                     /locus_tag="ACHJ1G_004041"
     CDS             complement(7322..9469)
                     /gene="cadA"
                     /locus_tag="ACHJ1G_004041"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4765804.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKELKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9549..10883)
                     /gene="cadB"
                     /locus_tag="ACHJ1G_004042"
     CDS             complement(9549..10883)
                     /gene="cadB"
                     /locus_tag="ACHJ1G_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4765805.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         3036 a         2618 c         2639 g         2861 t
ORIGIN      
        1 ttgaaattaa aaacaccgtt cacctgaaga gatattaatt tttagcgatg atggagggat
       61 aattatattt gatctggcac aagttttact gatgaaggat gtaacttgtg ccaggggtat
      121 tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat tggcaatatt
      181 gtttcagtag tgagtagtgt tctgcctgaa tacggtaacg gtaaaccgga cgccccgtga
      241 cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg aggtatttac
      301 ggcaggaaac acgcgagatg ttaacctcgt tagccagctc gtcggttgaa aactcatagt
      361 cctgatgcgc gtcaatccac tggcacagcg tgcgtaaagt ctgcggcgtt aagccttttg
      421 gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga tcaagctcgg
      481 cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag ccggtgagcg
      541 cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca ccgtaatgca
      601 gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca tcacttttgc
      661 aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc atatagatat
      721 cgagcaatat caggtcgata ggcgtatcgc tattgaagat aatctctttg gctttctcca
      781 gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat cggcgattca
      841 gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt tcgacctctc
      901 cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc ccgggttccg attccacggc
      961 gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga cgcctcgctc
     1021 gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg gtgcgattcc
     1081 cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca atgttacgct
     1141 aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca gatttcccaa
     1201 tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct ggctttcact
     1261 gtttaaaatc agcgtatggc ctaaatcggt cgcgcggtta atcttgctga ttaaaaaacc
     1321 agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct gatagttatt
     1381 ggctgttttg agaatgtaat cttccaactg cttataactc ttcagatgca ataatccgag
     1441 aatcacatgc aatttattca taaattcgtg ggatcgttca cgaagtgcat cagcatagtt
     1501 gaccagaccg tcgagtcgct gcatcagttt acgtacttca gttttgtccc tgaaggttga
     1561 aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca gtaatagccg
     1621 gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac
     1681 atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt cctgcgactt
     1741 acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc
     1801 cacggcaacg acgccttctt tgatagactg caacatggcc tggcgctgct caaacagcgt
     1861 ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa ccagaatgca
     1921 ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc tccagcgact
     1981 gtcattgatt tgttgggtca cgcggcttaa ctcaaggccg atcgccacca cgccaatttg
     2041 tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg
     2101 attgatagcg acattttctt tgccattcag cgctttaagg atgtcatcac ctttaaatgg
     2161 ctgaccaata cgctgggctt caggatgcga gtagcgaaga ctttgcatat cggtaacgac
     2221 aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga ttccgctctc
     2281 ctgcggtttt ttctgcaagc cctgacggat ttccggcgag tcggcgaggg tacgcgccac
     2341 tgccagtgcc ttgttggcca gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat
     2401 cagatgcacc accaatagca ccgagaacag taccgcgctg accattaaga tcactgtggt
     2461 actcaatttc atcggacgtt tgcgtaacat acggtagggc aatgaatgtc tcatcagctt
     2521 ccttgtgtga caaatttctt aagcattatc tctgatgagg cgggtaattc aaagggagta
     2581 agaatgattg gctatatagg ggaagagact ctggcaacgg aaactgccag tgctgtatga
     2641 agattccagg gctatgctta tagcgataat catactgatg agagagggaa agtcatggat
     2701 caggcgctac tggacgaggg ttatcgctgt tataccggcg aaaagatcga tgtctatttc
     2761 aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat
     2821 ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgtcactgt ggttaaagtg
     2881 attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg
     2941 aaatacgcga aggccacaat aaattttaca ttaatgacga acaaggcaag caaatcgctg
     3001 aaattgtctt tgtgccgacc ggagagaatt tagcgattat cgaacatacc gatgtcgatg
     3061 aaagcctgaa agggcagggg attggtaaac agctggttgc gaaagtcgtg gaaaaaatgc
     3121 gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa tttgataaaa
     3181 cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga tatttttcat
     3241 gctctccgcg gagtgatgca ggagagcatc tgaagggtag ggggatgcac aaagaatggg
     3301 cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa aagaatatct
     3361 cctatatgag aatcatcaat cggggttaat aatttttgcg tccccagagc attcaatatt
     3421 gataggagtc atattatgga aggtaaaaac aagttcaata cttatgttgt ttcttttgat
     3481 tatccatcat cttattcctc agtgttctta aggttaagat cattgatgta tgatatgaat
     3541 ttctcttcta tcgtggctga tgaatatggg ataccacgac aattgaatga aaattccttc
     3601 gcaataacga catcgttagc cgcaagtgaa atcgaagatt taatcaggct taaatgctta
     3661 gacttaccgg atattgattt tgacctcaac attatgacag ttgatgacta tttccgtcag
     3721 ttttacaagt agccaggaag gataagaaga acataaaaaa tggcactatt ctctaaaata
     3781 ttaatttttt atgtgattgg tgtgaacata tcctttgtca ttatctggtt tatctcacat
     3841 gagaaaacac atattcgttt acttagtgca ttcctggtcg gaataacctg gccaatgagt
     3901 ctgcctgtgg cattactttt ttctctcttt taggagctgt agttgagtgg acccgctggt
     3961 tatcgtgaaa cgaagcgtct ctcctcaaaa ctggatttgc ggaacgacga acgaagcagt
     4021 cgcctgactg cttcgttcat taaagtgaaa tttatttctg tgggcgcatc gccgggaaga
     4081 gaataacgtc gcgaatagta tgactgtttg taaacagcat aatcattcgg tcgataccaa
     4141 tacccagacc agcggttggc ggcagaccat attccagcgc agtcacgtaa tcttcgtcat
     4201 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
     4261 cctgatcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
     4321 agaattcaaa acggtcggtg atttctgggt taacatcatt acggcgtgcc agcggtgaca
     4381 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
     4441 caaagatctc cgtgacaata cgtcccagcc cccagctttt ctctaccgta ataccgatag
     4501 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
     4561 gatatttttt gattgcttcg cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca
     4621 catgctcgcc ataagtgact ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca
     4681 gggactctgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
     4741 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
     4801 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
     4861 gcaggtacat atctaaatcc agagcgttat gatgggtaat aaacgggcga gcagatgccc
     4921 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
     4981 actggcggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
     5041 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
     5101 atttatctgg taaaggacgt agtgcttttg tcagcaggcg caactcagta cagtgaatgg
     5161 aaagctcacc ggtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
     5221 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
     5281 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
     5341 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
     5401 ccttcgcatc aaactcttcg tgcagctggt cagaggtatg gtcgcggcga aaatcattgg
     5461 gaaacgccac accttgctga cgtagtgccg ccagtttttc gcggcgattt ctcagttcat
     5521 cgttaaaatc aatagcctca ttggctcccc gtgtttcttg ttcagacatt ttggttcctc
     5581 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
     5641 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agttgatatc
     5701 actaataaat aaccatcaaa atggtttaaa tgacttatta atctagattt gctagtgtta
     5761 taaactgaaa gttaatttat tcataaccaa ttgtttttac gacagttaat cgttgctctc
     5821 ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta gcaaaggcca atacgacgat
     5881 gatcgcgaca caagccaacg tccactctcc catctgagaa aagaatcgct ggtaagctgc
     5941 aattgccata ccgctaattt gcgactctgt cgtctgctgt gccacgacgc ctgccagcca
     6001 gttggcgacc gcgcctgttg ccagcatata aataccggtt aatacgccag acattttcag
     6061 acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt tcggcaaagc ccattagcgc
     6121 cagcccggat atcatcacgg ccattgacgc ttgaccgcca gccgctgcat gtcgggcatc
     6181 aaatgccaac aacataaagc cacaagccat cagcagtaag ccaaaggcaa acttcagcca
     6241 gacgcgcaat gttgagttgc cgcggctttc tggcgacgcc agccaggcca gtacaacccc
     6301 agcgagcatc actgcaattg cattcaccga ctggaatagt gctgtaggta cttcaatatt
     6361 gaatgcctga cgattcacaa agcgatcgat aaacaagctg atggtactgc cgccctgttg
     6421 tgccagtacc cagaacaacg tcccgacaaa catcaacaat acaatttgcc aaagagcacg
     6481 gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac
     6541 gatcgccagc aaatatccgg accagtcgtt ctccagcagc agggtaaaaa atactggggc
     6601 taaacagagc atcaccacta accagctcca tactggtaag gcaaatttca cgctagtgag
     6661 cgctttttta tccatactac gcgtggactg gaaatgacga tgcccgctta agaaaatcaa
     6721 caaaccgata aacatgccgc cacccgcaag ggcaaagcca acatgccatc cataccactg
     6781 agcagccagg ccgcaggcga ttggggcggc gatagaaccg atattgcccg cagcatacag
     6841 cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca
     6901 gctgatgttt gatttgaata aaccgtagcc acaaataatg attgccagcg ccagatacag
     6961 gctaaaggtt gaatttgtat caatgcccag caccacatgg ccaagggtca ttaacagcgc
     7021 gccggcaatc actgcagtgc ggttgccgag caggcggtcg gcaagccagc cgccgagaat
     7081 aggggtaacg taaaccagag aagcatatgc gctgaacagg ctgatggcat ggttatcatc
     7141 aaaaccaagc tgatgggtga gatagagaat gagtaaggca cgcatgccgt aaaaactgaa
     7201 gtactcccag atttggatcg ccacgatata gtatatcgcg cgcggctgtg agggtgtttt
     7261 catgtgttct ccttatgagc aaaaaaggga agtggcaagc cacttccctt gtacgagcta
     7321 attatttttt gctttcttct ttcaatacct taacggtata gcggccatca gcctgacggt
     7381 atgcaccgtg aatatcggtt tcaaagcccg gatagtgagc gccgatttca cacagcatct
     7441 gcaggaactc cagaaccgga cggctttctt cggtgatcat ttcacccggc attaccagag
     7501 gaactcccgg cggatacgga aggatcatat tggcgttaat acgacctacc atttcgtcga
     7561 ggtaaacttc ttcggtcata ccgtgcagct ctttctggaa tgcagcatac ggagtcatta
     7621 ccatcgtcgg cagcacttca aatgcgcggt acatcagatc cggcagattg tggtgaacaa
     7681 tcagtttgtg gatattctga gccagttcct gaatacgcat gttttcatag aattcaggat
     7741 cttcacgata cagagacggc agcatgtttt tcacacgcag gttcaggtcg aacgcacgtt
     7801 tgaagtcagt cagagcacgc agcaggctca gtgctttggt cttatcgata ccgatgctga
     7861 acaggaacag caggttatac ggaccggttt tctcaacaac gatgccatgt tcgtcgaggt
     7921 atttcgccac gatgctggcc ggaataccaa agtcgctcat ggtgccgtct ttttccatcc
     7981 ccggagtcag cagggtgact ttgatcgggt caagatacat gtgctcgtta tcgatgtttt
     8041 tgaagccgtg ccaggtgctg tcagaacgca gcggccagca ttcagtcgta tcgatatgat
     8101 ccggctgcca tacatcaaag aaccagccat cagattccgt tctcagacgt ttgagctctt
     8161 tacggaattt gatcgcacgt tcaatggaac cgttgatcag acgtttacct gcattgcctt
     8221 tcatcatcgc cgcagcggtt tcagtggacg ccacgatacc gtagtgcgga gaagtggtgg
     8281 tgtgcatcat gtaggcttcg ttaaaggttt cttcgtttac gtcaccttta acgtggatca
     8341 tggaagcctg agagaacgcc gccagcagtt tgtgagtgga ctgggtttcg taaatcactt
     8401 tcccttctac acggccaccg ctcataccgc atttaccttc gtaaatcggt gagaagttgg
     8461 tgtaaggcac ccacgcggag tcaaagtgga tggatttcac atccagtgtt ttcttgatga
     8521 agtcggtgtt gtacagcaga ccatcatagg tagagttggt aattacagca tgtaccggcc
     8581 aggttgcgtt tggtgtttct ttcacgcgct tagcaatggt agcgtgctgg aattcactct
     8641 gtgggatacc accaagaata ccgtaagcgt tacgggtcgg gcggaaatag attggcgtaa
     8701 catcgctcat catcatcagg tgggtcagcg atttgtggca gttacggtca atcagaatgg
     8761 tgctgcctgc cggagcagag tacatgccaa caattttgtt cgcagtggaa gtaccgttgg
     8821 tcaccatgta gctgcggtct gcgttaaaga cgcgagcgat atactgttct gcttctttgt
     8881 gtggaccact gtgatccagc agagaaccca gttcagatac tgaaatggaa atatcagatt
     8941 tcatggtatt cggaccaaag aaatcataga acaggctacc taccgggctt ttctggaatg
     9001 cagtaccgcc catgtgacca ggagtacaga aagtatattt accttcacga acatatttaa
     9061 acagtgcttt agtcagcgga ggcagaatag tgttgatata ttcgtcagtg gtctgcttga
     9121 tcttattagc aatatcttca gcagcaccca gcgcatattc aaagaagcta atctgtaaac
     9181 gcaggtcatt caggcttaca tcgagagtgg aatacgtatt agcgaacgcg tacaacggca
     9241 ggttctcgtt cattttgcta atttcttcgc acagctcgag attatattta tcccagtcaa
     9301 aaataacgcc gcacagacgc gcattgtttt cgatcagttt taataagtcg tcacggtcgt
     9361 tcgggtaaac aatctggaag ttcagacgtt caagcgcgcg atgaagttca cggatgggtt
     9421 cttctttaaa ataaaccccc atgtgattca atattgcaat aacgttcata gtcatatctc
     9481 caggtaaaaa aggcccctcc caacacatgg gacaaaatga aaggaggagc ctcggaaaat
     9541 acttttaatt aatgtgcgtt agacgcggtg tggttatcca ttgagtggct ctggcgctcg
     9601 tgcattttgc gagcgtagaa catcaggata atcaggctga cgatgaaggt acctgccagc
     9661 tcgaaggagc ttgcgcccat cagcgcgatg aagcagaaca cacaacccag tacagagcag
     9721 atcaggctga caaagttgcg gatgttaacg ccttcaaaac gaatcaggtc aacgcaagag
     9781 tagaagtacg gcagcatagt cagcagtact gcgataccgg tcagttcacc gaacaggtca
     9841 gatgctttac caccggcaga gttcatcaga gtgatgagga tcatcagggc agtcattttc
     9901 actgcagcca gcagcagacc ttttttcgga ataccgttgc tgtcgacttc accataaact
     9961 ttcgggaagt taccgtcgtt agcggcacgt acacctgcct ggcctaccaa catcatccag
    10021 gagcccagag aagtcaggca cgcaaaggcg gtgaatgcag aaaccagcgg cgcagcccag
    10081 ttaccgagga tggttgaagc actgattgca aacggagcac cggaagctgc cattacagaa
    10141 gatggataca taccggaaag cacctgagtc gcagcgatgt aaacaatacc tgctaaacca
    10201 gtacccagca tggttgccag cggaacggta cgtttcgggt ttttaaccat accagtactt
    10261 actgctgcgg attcaacacc cacgaaggcc cacaggcaga gcagaatact tttaatgatc
    10321 gcatgaccat cagtggtatc cgcagtattc cagttagctg cataagttgc cgcatcaaac
    10381 caatgccagc caacaatagc ggtcattact accggaataa gaaccagcac cagaccaata
    10441 gtggttaaac ggcttaccca agtaccgccg agcatattta caaaggtaaa tacccagacg
    10501 atagcaatac aggcgatacc cgccggaaca ggatcattta atactgggaa gaaggtggaa
    10561 agataagata ccgcggtaat accaatcgcc aggttaccaa tccagttagc atggtaataa
    10621 agaacacctg tctgaaaacc aaatgcaggg gaaatttctc cggcataagc aattgggcca
    10681 ccttgttgcg ggttttttgt tgccagtcgg gcatatacat acgccagcga cattgcacca
    10741 ataatagaga taatccaacc ccagatagca ataccaccga tacttgctag gttcgcaggt
    10801 aataatgcaa taccgctccc catcatatta ccggcaacaa caccggtaca ggcaaatagc
    10861 ccgatcttct tggcagaact catgctcttc tcctaatttc atttttgaat ttggagtccg
    10921 ggtcatgatg tataactatt tcctgaccag accaaactgg cgctaagaat actcacgaaa
    10981 aaaggattaa tcctaaagat taggtgaaat aacacaaaag tttctgtaag tgagaacttg
    11041 aggtttttta ttaacacatc aggatcgcaa gttgatatca tgaaaagata aacattcaat
    11101 gtttacaatg gattgcggga tattctctgg ttaaatttat gtaataaaaa ttat
//
LOCUS       DAZFDP010000148        11120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFDP010000148 DAZFDP010000000
VERSION     DAZFDP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..84)
                     /locus_tag="ACHJ1G_004043"
     CDS             complement(<1..84)
                     /locus_tag="ACHJ1G_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765806.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMT"
     gene            complement(159..809)
                     /locus_tag="ACHJ1G_004044"
     CDS             complement(159..809)
                     /locus_tag="ACHJ1G_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4765807.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            993..1583
                     /gene="espM2"
                     /locus_tag="ACHJ1G_004045"
     CDS             993..1583
                     /gene="espM2"
                     /locus_tag="ACHJ1G_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO4765808.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            complement(1606..1691)
                     /locus_tag="ACHJ1G_004046"
                     /pseudo
     CDS             complement(1606..1691)
                     /locus_tag="ACHJ1G_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1815..1964
                     /locus_tag="ACHJ1G_004047"
                     /pseudo
     CDS             1815..1964
                     /locus_tag="ACHJ1G_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2197..3060)
                     /gene="potB"
                     /locus_tag="ACHJ1G_004048"
     CDS             complement(2197..3060)
                     /gene="potB"
                     /locus_tag="ACHJ1G_004048"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4765809.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(3044..4180)
                     /gene="potA"
                     /locus_tag="ACHJ1G_004049"
     CDS             complement(3044..4180)
                     /gene="potA"
                     /locus_tag="ACHJ1G_004049"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4765810.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            4430..5659
                     /gene="pepT"
                     /locus_tag="ACHJ1G_004050"
     CDS             4430..5659
                     /gene="pepT"
                     /locus_tag="ACHJ1G_004050"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4765811.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(5805..6926)
                     /gene="roxA"
                     /locus_tag="ACHJ1G_004051"
                     /gene_synonym="ycfD"
     CDS             complement(5805..6926)
                     /gene="roxA"
                     /locus_tag="ACHJ1G_004051"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4765812.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            7175..8404
                     /locus_tag="ACHJ1G_004052"
     CDS             7175..8404
                     /locus_tag="ACHJ1G_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4765813.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGARPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQK
                     ALKPVTVGDALTYWLESYVKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGTHENVTTLPIAREI"
     gene            8779..8967
                     /locus_tag="ACHJ1G_004053"
     CDS             8779..8967
                     /locus_tag="ACHJ1G_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4765814.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCEAVH"
     gene            complement(9727..9906)
                     /locus_tag="ACHJ1G_004054"
     CDS             complement(9727..9906)
                     /locus_tag="ACHJ1G_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4765815.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKEQE"
     gene            10097..10297
                     /locus_tag="ACHJ1G_004055"
     CDS             10097..10297
                     /locus_tag="ACHJ1G_004055"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4765816.1"
                     /translation="MRNHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVGL"
     gene            10294..10524
                     /locus_tag="ACHJ1G_004056"
     CDS             10294..10524
                     /locus_tag="ACHJ1G_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765817.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            10514..10738
                     /locus_tag="ACHJ1G_004057"
     CDS             10514..10738
                     /locus_tag="ACHJ1G_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765818.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLTFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            10731..11096
                     /locus_tag="ACHJ1G_004058"
     CDS             10731..11096
                     /locus_tag="ACHJ1G_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765819.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
BASE COUNT         3066 a         2558 c         2709 g         2787 t
ORIGIN      
        1 cgtcattgtt gtttcatttg aaagttgttg atgttcactg aacagatggt aacatgatga
       61 tacaactgat gatattttgg gcataaataa tctcctcgct atatgtttgt tatatggtga
      121 ttaacttatt gtatgcttct tatattgtgt ttttattttt aaatactgtt ttgttgaagg
      181 gggtatatgg gtaatgcgta aaaacattgt tttgttatat taaaatgaca cttgtctttg
      241 cttataatca tttctggagc aaaggggtca cggctgaggg ggtggagagc gttacgcagg
      301 ataagttcag tcaatgcttc ctgatcatat aatgcacata tttctgaatt tcgagcattt
      361 ctgacaaaaa cacctgtctc aggaatatgt agcgttatcg gacaattaag atgctcctca
      421 gtacaggaaa agtcttgtaa actaacggga aatgcattcg aatttattcg atctagtagt
      481 gtttttatga cgctttcctg cacaagcggg gcagcttgcc tctgctccag atataagctg
      541 tatgcctgca gaaaagtgtt acctccgtta agttgtcttg ccaggctgtc tgcgagatga
      601 tgagactctc ttgccccaag atggtcccaa agtccaccag gaataggctc tacactaaaa
      661 ccatccataa actggatata atgaagcctc atattacggc ctcctatagt aatagtgtca
      721 ttctgattgc ttcttgcaat atgtcgtaaa gcctccagct cattttccgg caatacactt
      781 ccattagaaa aatttaatat gactggcata ttattatcct tttcaaccca ttttgtgaac
      841 tatattggta acaagagtca acataccgat catgagatgg gactcaaaca atacaacttt
      901 taatagaata ttatttgcat gcatgttttt tctggctaca ataaacacaa gcatgcaatc
      961 ttgtaaacgc ttttttataa aaaggaatta taatgccgat gaatacttca ggtacgtctt
     1021 tcagctcttt tggtataagt tgccatagag aaggctcctt taggaactct ttccgaggga
     1081 agaatgatga ggtcgtaaaa tgttcaattg gagaacggac tattagcttt tctgttcgca
     1141 aatttagcag aaatatactg gatacagtga acaggcagag tgctaaagat attgatgggt
     1201 ggataaaaga tgaacgcata gtatatccct caagggtgat caaccaggag attgataatt
     1261 actgttttca aaaaaatgca aaaatttcca ctgaagagag gcaaagagtc ttttctcttg
     1321 tgagccagga gaatcaacta acccttgatg ttaaggcagc acaaagttct attaatcatg
     1381 taataatggg gagtgcttct tttggcaaga aaatggatgc tctttgtgat ggtatgagcc
     1441 gggatgtaaa aaatcgtaca tcagatacca tagcaaactt gcttgcggac aagttttacc
     1501 agaaacatat cgattcggat attgatattg taaaactacg aaatgatatc ccagcttact
     1561 tgacgcgtgc tatacaggga tgaggctaga ggagaggact gcttcagcat catcggcttc
     1621 ggaccagtaa aagagacgca gaaaaatgca caacaggcac cacacgccat gcatggattc
     1681 agattactca tggctcacca gtacagctat aaatcgtaaa gaaaactaca gtacgttgta
     1741 cacagaaacg tagcgtgatt cactattgca caggtccatc acaacgacaa tattttgtga
     1801 tgtttgcgtg gtgcgcccca cttcaggatg ctgcagatct ggagatcgta attgaggaag
     1861 agacttcatt gcgggaagcc tggaaaaagt atcgggtgtt gttgaatcgt gttgatacat
     1921 cagcagcacc tgatattgag tggccgactt cacctgtaga gtaatcaacg gagcagggat
     1981 gatattttgt gcttgaagtg cctccccgac cattcagata gtcggggagg gggtattgcg
     2041 attatacgca atcagtttta gttaaagacc gaagctctcg aaaaatcttt ctccaaaacc
     2101 agaatgcaat tgattgattt tgataggatc tataacgccg ttaccttaaa cgacaaaatc
     2161 actcaaaaac aaacctaata aattgaatat aaaggattaa tcatctaatt cgttcacctt
     2221 cttattcagc agacgagaag cgcgccagta aaccagcaac atcaggccca ttacgatagt
     2281 cagcgtaatg ctggtggctg caccaaatgg ccagtcacga atattgagga actgtacctt
     2341 gatgacgtta ccgatcagca ggtttttcgc accgcccatc aggtcggata catagaacag
     2401 gcccatcgct ggcagcatca ccagcagaca tccggcaata attcccggca tcgtcagtgg
     2461 aataatgata cggataaaag tctgtaactt gctggcaccg agatcgcgcg ccgcctcaag
     2521 caacggctta tccagttttt cgatactgga gtacagcggc atcaccataa acggcagcag
     2581 aatgtaaacc agaccgataa tcaccgcact gggcgtgaac atgatacgga ttggtgtgtc
     2641 gataacgccc agccagagca aaaactcgtt gagatagcct ttggtgctga ggaaaatttt
     2701 cagcccgtag atacgaatca atgagttggt ccagaaggga acaatcagca gaaacagcag
     2761 cagcggacgc accttgtgtg gcagcttcgc cagaaaccag gcaaacgggt agcccagcac
     2821 caggcaggcg agggtggcga tcagcgccat attcagcgag tgcaatagca cttcaaaata
     2881 gagcggatcg agcagacgcg tgtagttatc cagcgtaaag accattttta cgaaactggc
     2941 gtcgtcgcgg gtcaaaaagc tggtgccaat gatcatcagg ttgggcagaa agacaaacaa
     3001 cacaagccaa ccgacaatag tgacaatcac tacattctgg aacttacttg tgttcttcat
     3061 cagccagtac gacctcccag ctttctaccc aattaatggc cattttttgg tcgagagagt
     3121 ggtcaaagtc aggatcgtct tcattgaaga attcgctgac catcaccatc ttgccatttt
     3181 ccagttcaac aaccgactcc agcgtcatgc ctttgtagtt acgctcacga acgtaaccaa
     3241 tcagcccttc agcgtggttg tcgtcgttaa tctcttcaac acgtaagtct tccgggcgca
     3301 gcagaacatg cagtttttgc cccggttcaa cggcgaagtt aacgtagata ttacattcgc
     3361 ggccttcaac gttggcgcgt acgcgctgct cgtctagacg ttcgatgaca gtggcgttaa
     3421 acatattgat ttcgccaatg aagccggcaa caaacaggtt tttcggctct tcgtaaattt
     3481 cgcgcggtgt accatcttgc tcaatacgac cctcgcgcat caccacaatc ctgtctgaca
     3541 tggtgagcgc ttcttcctga tcgtgagtga caaagacgaa ggtaatacca agcttacgct
     3601 gtaacgcttt cagctcgttc tgcatttgct tacgcagttt gtaatccagc gctgagagcg
     3661 actcatccag caacaacaga cgaggcttgt taaccaccgc gcgagcaatg gcgacgcgtt
     3721 gctgttgacc accagagagc tgatgcggtt tacgttgagc gaaggtttcc agttgcacca
     3781 tccgcagggc ttccatcacg cggggcgtaa tttcagcagc gggggttttt tgcatgcgca
     3841 acccaaaggc cacattttcg aacacggtca tgtgggggaa aagtgcgtag ctttggaaaa
     3901 cagtgttcac atagcggttt tccgccggaa cgtgggtgat gtcctcgtta tccagcatga
     3961 tgcgtccgga atcaacagtt tccagacctg caatcaggcg aagaacggtt gttttaccgc
     4021 agccagaagg gccaagcagc gtgaggaact cgccattgtt gatagtcaga tccagctggg
     4081 gaatgacctc tttaccatca aagcatttgc gaattcccgc caattgcacc agcggtgaaa
     4141 gcgaactcgg ttgtttattc aattttttac tctgtcccat gtaaacgcaa cggatggctt
     4201 accgatgcgg ggtttgtggt taaccacctt ggtgactctt aatgagggcg gtaattctac
     4261 ggcaaaccgc ttgaatcgcc aatctttgtt gtgaattact ggcttagctt tatattcatt
     4321 aaggtaatgc tgataaatat tcccgcttgc aggggtaaaa gtgacctgac gcaatatttg
     4381 tcttttcttg cttcttaata atgttgtcac aaaaagtgag ggtgactaca tggataaact
     4441 acttgagcga tttttgaact acgtgtctct ggatacccaa tcaaaagcag gggtgagaca
     4501 ggttcccagc acggaaggcc aatggaagtt attgcatctg ctgaaagagc agctcgaaga
     4561 gatggggctt atcaatgtga ccttaagtga gaagggcact ttgatggcga cgttaccggc
     4621 taacgtccct ggcgatatcc cggcgattgg ctttatttct catgtggata cctcaccgga
     4681 ttgcagcggc aaaaatgtga atccgcaaat tgttgaaaac tatcgcggtg gcgatattgc
     4741 gctgggtatc ggcgatgaag ttttatcacc ggttatgttc ccggtgctgc atcagctact
     4801 gggtcagacg ttgattacca ccgatggtaa aaccttgtta ggtgccgatg acaaagcagg
     4861 tattgcagaa atcatgaccg cgctggcggt attgcaacag aaaaacattc cgcatggtga
     4921 tattcgcgtc gcctttaccc cggatgaaga agtgggcaaa ggggcgaaac attttgatgt
     4981 tgatgccttc gatgcccgct gggcttacac tgttgacggt ggtggcgtag gcgaactgga
     5041 gtttgaaaac ttcaacgccg catcggtcaa tatcaaaatt gtcggtaaca atgttcatcc
     5101 gggcacggcg aaaggagtga tggtaaatgc gctgtcgctg gcggcacgta ttcatgcgga
     5161 agttccggcg gatgaaagcc cggaaatgac agaaggctat gaaggtttct atcacctggc
     5221 gagcatgaaa ggcaccgttg accgagccga tatgcactac atcatccgtg atttcgaccg
     5281 taaacagttt gaagcgcgta aacgtaaaat gatggagatc gccaaaaaag tgggcaaagg
     5341 gttacatcct gattgctaca ttgaactggt gattgaagac agttactaca atatgcgcga
     5401 gaaagtggtt gagcatccgc atattatcga tatcgcccag caggcgatgc gtgactgcga
     5461 tattgaaccg gaactgaaac cgatccgcgg cggtaccgac ggcgcgcagt tgtcgtttat
     5521 gggattaccg tgcccgaacc tgttcactgg cggttacaac tatcatggta agcatgagtt
     5581 tgtgactctg gaaggtatgg aaaaagcggt gcaggtgatt gtccgtattg ccgagttaac
     5641 ggcgcaacgg aagtcgtgat tgcccgatgc gtatgtggac tgccaattac cgggatgaaa
     5701 ccgttatatt gatactggtc cacatactta tcgtgcctac aggcgttcag tgcatgtagg
     5761 cacgataagg cgcaatcagc gccgcatccg gcttaaatcc aaacttaccc ttcgaagaac
     5821 caatacccgc tattgaccag cgccgcgagc atcgcgagga atgacggatc ttccagcgca
     5881 tcgccaaaat tctccgcagt cagcgcaatg ttgctggcga gtgcatccag tgccggacgg
     5941 tgcggggaat cgatcttctc accattggca tacacgtcgt cgccaatgcg caatacgcgc
     6001 agaccaccca ggcgcaccag cacttcacct tgtttcagcg catcgtagat ttcatctggc
     6061 tgataaggcg gttccggcgg cgcgatatcc agttcatgac gtgactggga tataaactcg
     6121 ccaaaccatt gcttaaagtg ttccggctgg ttgatcaatt cgagcatcat ctcacgcagt
     6181 ttatccatct cttgcggcag aacgtccgca ggatgagcgc gaggtggaac atccggatcg
     6241 ctgtagtagt tgccgcccag ttcacgttgc agcacataat cggcaaatcc gctaattaat
     6301 tcccgcgtat ttggcgcgcg aaaacccacg gaatagttca tcgcattttc cagcgcgtag
     6361 ccttcatgcg ggaatcctgg cggaatataa agaatatcgc caggctccag ctcttcatcg
     6421 atgatggctt cgaacggatc gacctgtaac agatccgggt gcggacagtg ctgtttcatt
     6481 tgcagctttt cgcccactcg ccagcgacga cgaccggtac cctgaatgat aaacacgtcg
     6541 tactgatcga gatgcgggcc gacgccgccg cccggtacag aaaaagagat catcagatca
     6601 tcaatacgcc agtccggtag ttcacggaac ggtcgcatca gcgcggcggt cggctcatgc
     6661 cagtggttca ctgcctgtac cagtaatgac cagttggttt caccgagatg atcgtagctt
     6721 tcgaacgggc cgtggctgac ctgccatttg ccatcctggt gactgaccag tcgactgtca
     6781 acttcgcttt ccatcgccag acccgccaac tcgtctggag agatcgggtc aataaaatta
     6841 ttaaagccgc gttttaacac caccgggcgt ttctgccagt gacgttcaag aaaatcgggc
     6901 cagttaagag tgagttggta ttccatgtta agcatccaca ggctggtatc tgcaaccgat
     6961 tataacggat gcttaacgta atgcgtgaag tatggacata ttttatcaat tagtgttttt
     7021 tgccgtttca taatgtttca gattgttcac tatgtttttg attataaaca gatttgtgat
     7081 tttccttgtt ccagcttgtt tcacgttgta gccctttgtg caacgatcgg tgtagttaat
     7141 ggtgtagtta attgtgatga ccggaatcaa aattatgagc agagcactta acaaactgag
     7201 cgatacacag ctgaggaaaa tcaacggcac acccgcccaa aaaacagcct ttcttaatga
     7261 cggtggaaac ctgagcgtca ggcattcaac cagcggcctt ttaacctggt atttcaccta
     7321 cagggccgga acgggaaggg gagcacgacc ggaacggatt aagctgggaa attatcctga
     7381 tctgagtctg aaagctgcca gggaaaaagc ggcacagtgt cgcgcttggc ttgctgaggg
     7441 gaaaaatcca cgtcatgaga tgaattacac cgtacagaaa gcgttaaagc ccgtaacggt
     7501 tggcgatgcg cttacctact ggcttgagtc ttacgtaaag gaaaaccgcg tggattatgc
     7561 cgccctgaaa aagcgcctta ataatcacgt aatacagcac attggtgcta tgccgctgga
     7621 taaatgcgag ctacggcact ggctggcctg ttttgaccag gtggcaaagc gaacgcctgt
     7681 tactgccgga ttcttgctac agacgtgcaa acaggcgctt aagttctgcc ggaggcggcg
     7741 ctatgcaatc agcaacgttc ttgatgatat gagtgtggcg gacgttggga aaaaaccgga
     7801 tataagcgag cgtgtcttaa gcaccaaaga acttggcgaa ttattgcagg cactggacaa
     7861 aaaaatattt tccccttact acgtcgcact aatccgcctc ctgattgttt ttggatgccg
     7921 gacggtagaa ctgaggttat cggagatcag cgagtgggat tttaccgaaa tgctctggac
     7981 cgtgccgaaa gaacacagca aaacgaaggt ggcaatattc cggcccatac cggaagcaat
     8041 actgccgttc gtcacgcagc tggtggagca gaacaggcac acgggcttat tgctggggga
     8101 agtgaaacag gaaacaagcg tgtcgcagta cggcagatta gcgcacagga ggctaaaaca
     8161 ccctcactgg tcactgcatg acatccggcg cacctttaca acgatgctga acgatttagg
     8221 cgtggatcct cacgtcgtgg agcagcttac aggtcatcag atgccaggga tgcaacgagt
     8281 ctataaccat tcccgttatc tggatgcgaa acgtgacgcg ctggatatgt ggacggagcg
     8341 gttagggata ctggcgggaa cacatgaaaa cgtaaccaca ttgccaatag ccagagaaat
     8401 ataatttttt tgcgattttt caggatgcgg acaacgtgaa acaacgagac acaacgacga
     8461 acaacaagga acaaaatgga acaacgggtt aaaaatcagc catcgtaata accatttgat
     8521 ttttataggt gtactgtttt tttatacact ggcgaatcac tcttaaaaat atgtaaaaaa
     8581 ggcgtaaaag taaaatttta tgcctttgca tcataatgaa ttaaaagagt ttttattctc
     8641 attccccctg cttctgtaaa acgaccgccg ccagtgtttg ccattccaca aacaccctga
     8701 cacaatccga cacaatccga cacaacccaa cccaataatc cacccaatga ctctttaaga
     8761 aacaacaaag ggggaattgt gttaagcaca gatcgcttta tacgtgaaaa agaatgtcag
     8821 caacttacag gcctgagccg ctcatgtcgg taccgcctgg aaaaagccgg acaattccca
     8881 tcacggcgta agctgggcgg tcgttccgtt ggctggtctt tatccgaagt tctggcatgg
     8941 aaggacagct gcgaggcagt tcactaatca ggctggcggc acacagccgc cacccatcca
     9001 ctaatacaga gatctaacca tgaatactgg atattctccc gaacaagggc ggggcttcgt
     9061 tcgtcctgaa aaacagaatc tgcaaaattt tgccgaaatt attccggtta tttccggcct
     9121 tactggcggg agtgaaacca atattgttaa cgccagagcg ttgcagatgt ttgatgataa
     9181 aaagggagta aatttaactt acacccctga cggcaatcag aatatgagca ttatctctga
     9241 gtcaggtttc tacaaactaa taaaaacaaa aagcgccccg ttgccggagc gcctttgtga
     9301 acaattaacc tactgcgcaa aaaatgaatc tgagcagtgg gattatatca accatgtgga
     9361 gaagcgccac aattgccgaa taacgggcaa aacaaaggcc acccgctacg gtggcccctc
     9421 gacacaagct acacgttatc cccaacgcat gagcattgcc aacaatgcca catttgcggc
     9481 tggtgggcaa tgcaatcagt ctggttcagt tcgttgccat acctgcaatg agcgcttttc
     9541 cctgtactct ttaaggaatt gctcaagggc aaaagcacat ggcgcgaatc tttctgattc
     9601 atgctctatc tttctgcgcc gtcttttccg tgccggtgat aatgttttgg tcaattcttt
     9661 atcggtcatt gtgttgtcct gcatagcaat gcgccgtagt tactcacacc acggcgctgg
     9721 tgatggttac tcctgctctt tggccttgcg acgctggcgg cgtttgatct cacctttaac
     9781 ggcagtaact ataaattgcg ccttgctttc accttcatct aaattttttt ctaagtctgc
     9841 aacaacatca tgcgggaatc tggcatttaa ctgttgcgac ttattatttg ttgaacctgt
     9901 tgccattact gaatcctctc taagttggtg cgattcagta tacacaaaaa aaatttcaga
     9961 gaaagacttg aagtgcgatt cacttactga taggttaaaa acgaatggtg cgatgcacca
    10021 aacaacaacg ccccgcagtg gtggcacaca tgcagggcgt ctaaccacca acgatagcga
    10081 aagtatcgag gtagctatga gaaatcatac catacacccg caagggcggg actcgcacaa
    10141 cctgaataaa tacatctggc gttttatcgc cctgagcacg gcacaaccgc gcgtgattac
    10201 cattgaggcc accagcgaac aggaagcacg ccagcaatct ccggctggct gcgtgatggt
    10261 atttgctgcc cgtattcgtc agggggtagg cttatgagcc aggaaatcac actacaacag
    10321 gcagcagaac gcgcccacca aatcgaagtt atttgcgcac tggcagagga ttaccctggc
    10381 atgatgaccg acagcgaatc aggggcaatc atcggcttac ttaaacgcct tagcggtgag
    10441 gtctgcgtat tcctgagcga tgaacaggaa agaagaacgc ttatttctaa cgaaaaaaaa
    10501 tgcggagggg tacatgtgca ataaaaccac accggacgca gccgccgccg cgctcactac
    10561 gctgatgcac gcgcttattg atatttctgt tattgctgac agggcgcata aacacgcagc
    10621 cagagaatca gaatgcatct tccattattt aacatttgtg cagctaaaag ccgatcaggc
    10681 actggataag gccggaaaaa ttatcatggc tgatgtgcag gaggtgcacc atgcataacc
    10741 tgtcaatttc tgaccttaac agcattcagt ttgacgagaa atttaccggg cagttgctgg
    10801 tcaatgtgga gaacgggcgc atagtgcgta attatcacct gccggatggt gcaattgccg
    10861 gaagcgttga agcattgctg gaactggcgg aacgtgcgcg actgattaag ccgtcaacga
    10921 gccatcacga tgatgatctg cattttaccg gatgtatggt gagtcactac gaaaacggcg
    10981 tcgaggtatc ctgcgaacgg ctgcgtgatg attgctgttt cggcacactg ccggaattta
    11041 tcgagttgct gaccagttgc ggttatcagg tcattcaggg gggtaaacat gcgtgatgat
    11101 cgttttaatt ccctgaaaca
//
LOCUS       DAZFDP010000149        10997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFDP010000149 DAZFDP010000000
VERSION     DAZFDP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(202..2469)
                     /gene="adiA"
                     /locus_tag="ACHJ1G_004059"
     CDS             complement(202..2469)
                     /gene="adiA"
                     /locus_tag="ACHJ1G_004059"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4765820.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2668..3576)
                     /gene="melR"
                     /locus_tag="ACHJ1G_004060"
     CDS             complement(2668..3576)
                     /gene="melR"
                     /locus_tag="ACHJ1G_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4765821.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3859..5214
                     /gene="melA"
                     /locus_tag="ACHJ1G_004061"
     CDS             3859..5214
                     /gene="melA"
                     /locus_tag="ACHJ1G_004061"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4765822.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5317..6738
                     /gene="melB"
                     /locus_tag="ACHJ1G_004062"
     CDS             5317..6738
                     /gene="melB"
                     /locus_tag="ACHJ1G_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4765823.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6877..7506)
                     /gene="yjdF"
                     /locus_tag="ACHJ1G_004063"
     CDS             complement(6877..7506)
                     /gene="yjdF"
                     /locus_tag="ACHJ1G_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4765824.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7629..9275)
                     /gene="fumB"
                     /locus_tag="ACHJ1G_004064"
     CDS             complement(7629..9275)
                     /gene="fumB"
                     /locus_tag="ACHJ1G_004064"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4765825.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9353..10693)
                     /gene="dcuB"
                     /locus_tag="ACHJ1G_004065"
     CDS             complement(9353..10693)
                     /gene="dcuB"
                     /locus_tag="ACHJ1G_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4765826.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2581 a         2684 c         2903 g         2829 t
ORIGIN      
        1 acaaattttt atagtaattt ctcatgtaca tattttttta caggataaat aacaggcaaa
       61 aaaaagcgcg gggtggggcc gcgcaaaaag tatgacattt ttgtttgcag caatttttat
      121 gaaaacgttg ccatactcaa aaggcctaag gccaggcggc gtaatgttat ttaaacaatt
      181 acgccttcag cggaatagtg gttacgcttt cacgcacata acgtggtaaa taccgtcaat
      241 aatttcagtc ccttcagttt cgtgttcaaa tccagggaaa tggtggtccc aggattgcag
      301 cgagcgtaaa taacttactt gcggactgtt tttatcgccg aagttttcac cagacagcag
      361 catcgggatt cctggcggat acgggataac tgagtttgcc gcgatgcgtc ctggcagatt
      421 ttcaatggat accagttcga cattgttgtc gacaatcgcg ttgtacgctt cacgcggggt
      481 gatttccgcc atcggcaggc cggaataggc ttcgttcaac cgtgcgccag ggttgttttc
      541 tttcagccag gcaaacatgg tgtcacccag atcgtgaatc cccatgttcg cgtaagtgtc
      601 aggatattgt tcaacaagtt ccggcatcac ctgcgccagc ggtgtgttgg cgtcatagtg
      661 gcgtttgaag gaacaaaggg tgttaaccag agtcccccat ttcccacggg ttacgcccat
      721 agagaacagg aacataattt ggaagtcagt ggtgcgggta ggcacaatgc cgtggcgacc
      781 aagccaggca gtgaccagcg ccgccggaac accggtttct tccagttcac catcttcacc
      841 cattcccgga gcaaggatgc tgactttaat tgggtcgagc atactccagt tatccggaat
      901 atctttgaag ccgtgccagc tttcgcccgg atgcattacc cagcagtcct gaacggtggt
      961 cagcagtttg gttggtgcgt cagcaaagtc ataggttttg ccggtttgtg ggtcggtgac
     1021 gacttctttg ttccacggtt tgaagaacca gctaccgtca gcggtgaact ctttatatag
     1081 ccgcgccatc gcctgacgga aatcaaccgc ttcgtcaatc acttcctgtg tcagtgacag
     1141 gccgctgttg ccgtccatca tcgacaccgc cacgtcgttg gatgcgcaga tggcatacag
     1201 cggggaggtg gtggcatgca tcatgtaggc ctggttgaag cgggagaagt taatcgcccc
     1261 acgaccttca cgtacatgaa tataagaagc ctgtgacagc gcattcagca gtttgtgggt
     1321 ggagtgggtg gcgaaaacgg taggaccgtt gtgatcgcca ggttcgccgc gcatggcata
     1381 gtgatcggca tagatcgggt tgaaacgtgc atagccgtac caggcttcgt caaagtgcag
     1441 acgatcggag gttttttcca gcagatcctg cgcttcttta gcgttataac acacgccgtc
     1501 ataggtgcag ttggtcacca cgcagtaaga cggtttttgc ccggctttgt ctttggtcag
     1561 cgggctttca ctgattttct tctgcaaggt ttcaggttgc atttcctgcg gatagattgg
     1621 cccgataatg ccgtagcggt tgcggcttgg caccatatag accggtttcg cgcctgtcag
     1681 catcaagcct tgttcgatgg atttatggca attacggtca acgaccacga catcgttatc
     1741 ggtcatgcaa gcctgcatga tggtgcggtt agagccggaa gtaccgacga ctaccgacca
     1801 ggagcgatcg gcaccaaata cgcgtgcggc atatttttcg ctttcgccaa atgcgccagt
     1861 atggtcaagc aaagaaccga gggaagttcg ttcgatgccc atgtcggtgc ggaacagatt
     1921 ttcaccatag tagtcatggt agaaacgtcc ggcgggtgtt ttggtaaaac caacgccgcc
     1981 ctggtggcct ggcgctgccc aggaatattc atggatgtca ctatatttca tcagcgcgct
     2041 gaacagtggc ggcaacagct gctggcggta gcgggtcatc gcggcaacgg cgcgtccggc
     2101 gataaagtcg gcggtatctt ccagaatcca ggcgaattca tcgacaagct ccagcagatc
     2161 ccggtccatt gcggcgaggg ctttttcccg atcgcccaac aggaagaccg gcacgttttg
     2221 ttggcgctca tgaagcttac cgatcaattg tctgacgttt tgatgttcgt ccggatgttc
     2281 catttgatag ctgaacatca ggcagtcaat ggcttcgttt gaagagagaa tggcaaaacc
     2341 atcatcaaag gaggtggatt taatcacggt aacattttgc tggcttaaag catctgccag
     2401 acgctcaacg gcgttaccga cccaggtgtc ttgatggaga aactcgcttt caacaattaa
     2461 tactttcatc attgcttacc cggttatgaa ggaatcttcg ttgcgggcaa gtatcttccg
     2521 gccttttcgg gctgtaaagc gcgtgaaaaa caaacatttt tgaaatattt agttttataa
     2581 acaatacgtt atgtgattat tttaaattat tttcactgct tattatcgac ggctaaacta
     2641 tttttttggc tgatactgat atcgtcttta gccgggaaac gtctggcggc gctgttggct
     2701 aagtttgcgg tattgttgcg gcgacatgcc gacatatttg ccgaacgtgc tgtaaaaacg
     2761 actacttgaa cgaaagcctg ccgtcagggc aatatcgaga atacttttat cggtatcgct
     2821 cagtaacgcg cgaacgtggt tgatgcgcat cgcggtaatg tactgtttca tcgtcaattg
     2881 catgacccgc tggaatatcc ccattgcata gttggcgtta agtttgacgt gctcagccac
     2941 atcgttgatg gtcagcgcct gatcatagtt ttcggcaata aagcccagca tctggctaac
     3001 ataaaattgc gcatggcgcg agacgctgtt tttgtgtgtg cgcgaggttt tattgaccag
     3061 aatcggttcc cagccagaga ggctaaatcg cttgagcatc aggccaattt catcaatggc
     3121 gagctggcga atttgctcgt tcggactgtt taattcctgc tgccagcggc gcacttcaaa
     3181 cgggctaagt tgctgtgtgg ccagtgattt gatcaccatg ccgtgagtga cgtggttaat
     3241 caggtcttta tccagcggcc aggagagaaa cagatgcatc ggcagattaa aaatcgccat
     3301 gctcttacag gttccggtat ctgttagttg gtgcggtgta caggcccaga acagcgtgat
     3361 atgaccctga ttgatattca ctttttcatt gttgatcagg tattccacat cgccatcgaa
     3421 aggcacattc acttcgacct gaccatgcca gtggctggtg ggcatgatat gcggtgcgcg
     3481 aaactcaatc tccatccgct ggtattccga atacagcgac agcgggctgc gggtctgttt
     3541 ttcgtcgctg ctgcacataa acgtatctgt attcatggat ggctctcttt cctggaatat
     3601 cagaattatg gcaggagtga gggaggatga ctgcgagtgg gagcacggtt ttcaccctct
     3661 tcccagagag gcgaggggag cgtccgagca tcatgaggcc gaaaactctg cttttcaggt
     3721 aacttattac cataaactca gatttactgc tgcttcacgc aggatctgag tttatgggaa
     3781 tgctcaacct ggaagccgga ggttttctgc agattcgcct gccatgatga agttattcaa
     3841 gcaagccagg agatctgcat gatgactgca cccaaaatta catttatcgg cgctggttcg
     3901 acgattttcg ttaaaaatat tcttggtgat gtgttccatc gcgaggcgct gaaaacggcg
     3961 catattgccc tgatggacat tgatcccacc cgcctggaag agtcgcatat tgtggtgcgt
     4021 aagcttatgg attcagcagg ggccagcggc aaaatcacct gccacaccca acagaaagaa
     4081 gccttacatg atgccgattt tgtcgtggtg gcatttcaga ttggcggtta tgaaccttgc
     4141 acggtgactg atttcgaggt ctgtaagcgg catggtctgg aacaaaccat tgccgatacg
     4201 ttggggccgg gcggtattat gcgcgcgcta cgtaccattc cgcatctgtg gcaaatttgc
     4261 gaggacatga cggaagtctg ccccgatgcc accatgctca actatgttaa cccaatggcg
     4321 atgaatacct gggcgatgta tgcccgctat ccgcatatca aacaggtcgg gctgtgccat
     4381 tcggtgcagg gaacggcgga agagctggcg cgtgacctca atatcgaccc agctacgctg
     4441 cgttaccgtt gcgcaggtat caaccatatg gcgttttacc tggagctgga gcgcaaaacc
     4501 gccgacggca gttatgtgaa tctctacccg gaactgctgg cggcttatga agcagggcag
     4561 gcaccgaagc cgaatattca tggcaatact cgctgccaga atattgtgcg ctacgaaatg
     4621 ttcaaaaagc tgggctactt cgtcacggaa tcgtcagaac attttgctga gtacacaccg
     4681 tggtttatta agccaggtcg tgaggatttg attgagcgtt ataaagtacc gctggatgag
     4741 tacccgaaac gctgcgtcga gcagctggcg aactggcata aagagctgga ggagtataaa
     4801 aacgcctccc ggattgatat taaaccgtca cgggaatatg ccagcacaat catgaacgct
     4861 atctggactg gcgagccgag tgtgatttac ggcaacgtcc gtaacgatgg tttgattgat
     4921 aacctgccac aaggatgttg cgtggaagta gcctgtctgg ttgatgctaa tggcattcag
     4981 ccgaccaaag tcggtacgct accttcgcat ctggccgccc tgatgcaaac caacatcaac
     5041 gtacagacgc tgctgaccga agctattctt acggaaaatc gcgaccgtgt ttaccacgcc
     5101 gcgatgatgg acccgcatac tgctgccgtg ctgggcattg atgaaatata tgctcttgtt
     5161 gacgacctga ttgccgccca cggcgactgg ctgccaggct ggttgcaccg ttaaaacgcg
     5221 actaaacgct actgcgccgg gggatttatt ccggcgcaca cctctgacga taccaataac
     5281 agaaggcggg cgttggtaac agcgacccga taccctatga gcatttcaat gactacaaaa
     5341 ctcagttatg gatttggagc gttcgggaag gattttgcga tcggcattgt gtatatgtac
     5401 ctcatgtatt actacaccga tgtcgttggg ctgtctgtgg gtttggtcgg tactttgttt
     5461 ctggtggcga ggatctggga tgctattaac gatccgatta tgggatggat tgtaaatgct
     5521 acgcgatcgc gatggggtaa gttcaaaccc tggatcctga tcggtacgtt ggcaaactct
     5581 gtaatcttat ttctcctctt tagtgcgcat ctgtttgaag gtactactca gattgtcttt
     5641 gtttgcgtga cctacatcct ctggggcatg acttacacca ttatggatat tcccttctgg
     5701 tcgctggttc caaccatcac gctcgataaa cgtgagcgcg aacaactggt tccttatccg
     5761 cgtttttttg ccagtctggc aggctttgtt acggcaggtg tgacgctacc atttgttaat
     5821 tatgtcggcg gtggcgatcg gggatttggc tttcagatgt tcactctggt actaatcgcc
     5881 ttttttattg tttcaaccat catcactctg cgcaatgtgc atgaagtctt ttcgtcagac
     5941 aatcaaccgt ctgctgaagg aagccatctg acacttaaag ccatcgttgc gctaatttat
     6001 aaaaacgatc agctttcatg cctcttgggt atggctcttg cttataatgt agccagcaac
     6061 attattaccg gatttgctat ctattatttc tcatatgtta tcggtgatgc ggatttgttc
     6121 ccctattatc tgtcgtatgc gggagctgct aacctggtga cgttagtatt cttcccacgc
     6181 ttagttaaat cattatcccg acgcatttta tgggccggag catctattct tccggtgtta
     6241 agctgtggtg ttctcctgtt aatggcatta atgagctatc acaacgtcgt cctcattgtg
     6301 attgcgggta ttttgctgaa tgtgggaacg gcgcttttct gggtattaca ggtcatcatg
     6361 gtggcagata ccgttgatta cggtgaatat aaactgcacg tacgctgtga aagtatcgct
     6421 tactccgtgc agactatggt ggtgaagggc ggttcagcct ttgcggcttt tttcattgcg
     6481 gttgtgttag ggatgattgg ctatgtaccg aatgttgaac agtctacgca agccctatta
     6541 ggtatgcagt ttattatgat tgctctacca actctgtttt tcatggtaac gctgattctc
     6601 tacttccgtt tctatcgcct caatggcgac acgctgcgca ggatccagat tcatctgctg
     6661 gataaatatc gcaaagtacc gcccgagcct gttcatgctg atattccggt cggtgcagtg
     6721 agtgatgtga aagcctgacg tggacaatag ctaacgaaaa tggcctgatg cgatatgttt
     6781 atcaggccac gtcttaatgt aatgctttgg atttgcaaat tttgtagacc ggataaggaa
     6841 ttcacgccgc atccggcatc aacaaagcgc acgttgttat ccggttatca agccaaagcg
     6901 ccgtagctgg cggcaatgaa agcgagcgag gaatatcacc gtcgttaatg cgccaagcag
     6961 cgcgcagaac atatcggatt gcgtgtccca ctggtcgccc tgggtgccga gaaaatcatc
     7021 cgctccctgg cccattgcca gcgccgccca ccactcgatt aattcataca tcgcgcttat
     7081 cgccagcgcc acgcagcaga ccaggaacgc caccatttta cgtccgcgca cgtacatccc
     7141 gcgcacgaga atttctcgtg ccaccagtgc aggcaccagc ccctggaaaa aatgccccag
     7201 cttgtcatac ggattacggc tcaaccccag ccattcctgc acctcaaaac cgatggggac
     7261 tttcgcgtag gtgtattgcc cgccgaccat caggatgatg gcgtgaaaga aaatgagcgt
     7321 atagagcagc ggcgttaacg gataacgttt agcggtggca agcagtagcg gcacgacaat
     7381 aatcaccggt gtcacttcca tcagccaggt gagtttgtca tgggccgaaa tgccggtata
     7441 aatcaggatt aacgttagcg ccagtgcgcc ggtgttaaga attaacggct tgagtgtacg
     7501 ggtcatggtg agttcgcaag tcagggaaaa tactgactat tcaccagcaa gcgtgaaatt
     7561 acaatcgcaa aagaggtggc ccagggggat cacctggcag catgctgcca ggcgctgggc
     7621 cgaagaggtt acttagtgca gttcgcgcac tgtttgttga cgatttgctg gaagaagtcg
     7681 ttacctttgt catcgaccag gataaacgcc gggaaatctt ctacttcgat tttccagata
     7741 gcttccatac ccagctccgg ataagcgacg cactccagat gcttgatgct ctgctgcgcc
     7801 agtaccgccg ccggaccgcc gatgctaccg aggtagaagc cgccgtgttt atgacacgcg
     7861 tcggtaacct gctgactgcg gttacctttc gccagcatga tcatgctgcc gccgtgggat
     7921 tgcagcagat ccacgtagga gtccatacgg cctgcggtgg ttgggccaag tgaacctgat
     7981 ggataaccgg caggggtttt cgccggaccc gcgtagtaga tcgggtgatc tttgatgtac
     8041 tgcggaagtt ctttaccggc gtcaatcagc tctttcagct tggcgtgtgc aatatctcgg
     8101 cccacgataa tggtgccggt gagcgacaaa cgagtggata ccgggtattg cgaaagctgg
     8161 gcgaggatct ctttcatcgg gcggttaagg tcaactttca ccgcttcgcc ttcaccggcc
     8221 tggcgcagtt cttgtggaat gtactggcct gggttgtgtt ccagtttttc gatccagata
     8281 ccttcgcggt tgattttcgc tttaatgtta cggtcagcgg agcaggagac gcccatgccg
     8341 accgggcagg atgcgccgtg acgtggcaga cggataacgc gaatgtcgtg cgcgaagtat
     8401 ttaccgccaa actgcgcgcc aagaccgagt ttctgggcct cttccagcag ttcctgttcc
     8461 agctggacat cgcggaacgc ctgaccatgt tcgttccctt ccgtcggcag ttcatcgtaa
     8521 tagtgagcgc ttgctaactt gacggttttc aggttggttt ccgcagacgt accgccaatc
     8581 acaaacgcga tatggtacgg cgggcaggct gcagtaccga gggtacgcat tttctcgacg
     8641 aggaagtttt tcagtttgcc gggagtcagc agggctttgg tttcctggta gagatacgtt
     8701 ttgttggcag agccgccgcc tttcgcaacg caaaggaatt tgtactcatc gccatctacc
     8761 gcgtacaggt cgatttgcgc aggcaggtta gtgccggtgt tgacctcttt gtacatgtcc
     8821 agcgccgcat tctgtgaata gcgcaggtta tcttcgatat aggtgttata gacgcctttc
     8881 gacagcgctt cttcatcacc gccaccggtc cacacgcgct ggcctttttt accgacgatg
     8941 atcgcggtgc cggtatcctg gcaggtcggc agcacgcctt tggcggcgat ttcggagttt
     9001 cttaagaatt gcagcgccac gtacttgtcg ttttcgctgg cttctggatc gtgaagaata
     9061 gccgcaacct gtttctggtg tgccggacgg agcatgaaag aagcgtcgtg aaaggcttgc
     9121 tgcgccagta gggtcagggc ttctggttcc actttcagga tggtttcgcc gtcgaagtcg
     9181 gcaacgctaa cgtaatcgga agtgagtaga tagtattcgg tattgtcttt ccccatcggg
     9241 aaaggtgcct ggtagataaa gggtttgttt gacatagctt ccagcctgta actctgtatt
     9301 tgttattcga aaatggcgtg ccgcgtagtc acgggcggca cgcaaagtgc atttataaga
     9361 acccgtacat cgcggcgaag atccagccga agacgcacga tacgctcaca ccaatcaacc
     9421 ccggcagaat aaaactgtgg ttgatgacga agcgaccgat gtgggtggtg ccggaacggt
     9481 caaactgaat cgctgccaga tcgctcggat aagtcggcag gatgtaataa ccgtagcaag
     9541 ccggtgctga agccacgatg tatgccggat caacgccgat cgccagcgcg accggaacaa
     9601 tcgccgccag cgccgcagcc tgagagttta caaacttgga aaccagcagc agaacaatgg
     9661 catacgccca cggatactct ttcaccattt cacccagtac gccctgaatt tcagacatat
     9721 gcgcaccgaa catggtttct gccatccatg cgataccgta caccgccacg atggcgatca
     9781 taccggaacg gaagacttcg ttttttgaga tagacgcggg attggttttg gtcaggataa
     9841 taatcagcgc cccggtcagc agcataaaca tctgaataac cagtaccatc gacagcggtt
     9901 tgccgccgaa ggatggacgc aggtccgaat cagcaccaag aagggcgact acagcgattg
     9961 caccgaggaa aatccacatt gccagccagt tgcttttcgg cagtttttta tcgagcagcg
    10021 tcgcggtatc accgtaaaca tactcacggt tttccggtac ggagatgaat ttctggaact
    10081 cttcgtcttt atccagatct ttaccgcgga accagctgaa gataccgatc gccaggatac
    10141 cgattaacgt cgatggaatg gtgattgcca gcaggtcgag gaactcaaga tggcgaccat
    10201 caaaggtgac attacccagc attgcaacca gagacacgac cgcaaccgac accggactgg
    10261 cgataatccc catctgtgca ccgatagaac ttgccgccat cggacgttcc ggacggatgt
    10321 tgttcttaat ggcgacgtcg tagatgatcg gcagaatggt gtaaaccaca tgacccgtac
    10381 cgcaaagaat ggtcagtgta caggtcacaa acggcgcgac aattgagaca tatttcgggt
    10441 tgcggcgcag cagcttctcg gcaatttgca gcatgacatc aagaccgccc gaagcttgca
    10501 aggtcgccga tgccgccacc accgcaatga taaccagcat gacatcaacc ggtggtttac
    10561 ctggctgaag gtggaagacg aagaccagaa tgaccagacc gataccgcct aataaaccca
    10621 gcgcgatacc accctttctg gcaccataaa acagacatat cagtattatg ataagttgga
    10681 tagtaaataa catgtgtgaa ccctcgcgat aatcctattt aaatttttgc tgaatagatc
    10741 acagtcacgt tctgttttgt atgaactgtt ttcagagcct gactaaatat ccgtttggtc
    10801 gttaccggct ttagcaaata cctcacagta aatattggct gattaaagca gcggcaaatt
    10861 tatgcactgt gataagcggc tttttcagag agaaaaactc tctggcgatg ggtttatagt
    10921 ttgtactgcg cagatgttaa tgttttgttt gctgttatct tattgatatt tatcggtcta
    10981 ttttttgttc tcagggc
//
LOCUS       DAZFDP010000150        10967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFDP010000150 DAZFDP010000000
VERSION     DAZFDP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..1870)
                     /gene="glmS"
                     /locus_tag="ACHJ1G_004066"
     CDS             complement(41..1870)
                     /gene="glmS"
                     /locus_tag="ACHJ1G_004066"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4765827.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2032..3402)
                     /gene="glmU"
                     /locus_tag="ACHJ1G_004067"
     CDS             complement(2032..3402)
                     /gene="glmU"
                     /locus_tag="ACHJ1G_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4765828.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3753..4172)
                     /gene="atpC"
                     /locus_tag="ACHJ1G_004068"
     CDS             complement(3753..4172)
                     /gene="atpC"
                     /locus_tag="ACHJ1G_004068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4765829.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4193..5575)
                     /gene="atpD"
                     /locus_tag="ACHJ1G_004069"
     CDS             complement(4193..5575)
                     /gene="atpD"
                     /locus_tag="ACHJ1G_004069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4765830.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5602..6465)
                     /gene="atpG"
                     /locus_tag="ACHJ1G_004070"
     CDS             complement(5602..6465)
                     /gene="atpG"
                     /locus_tag="ACHJ1G_004070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4765831.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6516..8057)
                     /gene="atpA"
                     /locus_tag="ACHJ1G_004071"
     CDS             complement(6516..8057)
                     /gene="atpA"
                     /locus_tag="ACHJ1G_004071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4765832.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8070..8603)
                     /gene="atpH"
                     /locus_tag="ACHJ1G_004072"
     CDS             complement(8070..8603)
                     /gene="atpH"
                     /locus_tag="ACHJ1G_004072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4765833.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8618..9088)
                     /gene="atpF"
                     /locus_tag="ACHJ1G_004073"
     CDS             complement(8618..9088)
                     /gene="atpF"
                     /locus_tag="ACHJ1G_004073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4765834.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9150..9389)
                     /gene="atpE"
                     /locus_tag="ACHJ1G_004074"
     CDS             complement(9150..9389)
                     /gene="atpE"
                     /locus_tag="ACHJ1G_004074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4765835.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9436..10251)
                     /gene="atpB"
                     /locus_tag="ACHJ1G_004075"
     CDS             complement(9436..10251)
                     /gene="atpB"
                     /locus_tag="ACHJ1G_004075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4765836.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10260..10640)
                     /gene="atpI"
                     /locus_tag="ACHJ1G_004076"
     CDS             complement(10260..10640)
                     /gene="atpI"
                     /locus_tag="ACHJ1G_004076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4765837.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2605 a         3007 c         2724 g         2631 t
ORIGIN      
        1 gggccaccgg tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt
       61 ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca
      121 gcagctgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct
      181 cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca
      241 actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt
      301 tgttcggtgc aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt
      361 tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg
      421 cgccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt
      481 cagagaaatc ttctgccaga gcttcaatgc gtttgtcctg agacagcatc tgctcaatac
      541 ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt
      601 tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt
      661 tagtggatgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc
      721 gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt
      781 tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca
      841 tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg atttcgacgt
      901 cgcacggaat acctgccagc gattcaaacc agtaacggga aaccataccg gagttataag
      961 aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg
     1021 gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttga
     1081 tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc
     1141 ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat
     1201 cgaagatgtt taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa
     1261 agcgacgggt caccggcagc agcgccagct ggtcagaagc gataaagttt tcgcccatac
     1321 ccagaccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg
     1381 agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc agaacagcct
     1441 cacgcagagt accgccctgt ttcagctccc agttcaccag atgggcaatt acttcggtgt
     1501 ctgtttcaga aacgaaggta tagccacgcg cttttaactc ttcgcgcagc ggttcatggt
     1561 tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcattca
     1621 cttctgaagg ttcaccgtgg gtcgcccagc gagtatgagc aatacccgta ccgccatgca
     1681 gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg
     1741 tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca
     1801 gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa
     1861 ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt
     1921 tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga
     1981 gggagattat gttatctcct catcccatgt gaccggatta cccggccaga atcacttttt
     2041 ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc
     2101 attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac
     2161 tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt
     2221 cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt
     2281 atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag
     2341 acgcgctttt ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc
     2401 aggacgcagt cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac
     2461 aacggtatac ggactgattt cgcaatcatc accaatcacg ctgtttttaa tcacgcaacc
     2521 ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc
     2581 aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaaa tcaaaacgcg ccggatcgcg
     2641 cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag
     2701 acgggagagt tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac
     2761 ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc
     2821 gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc
     2881 gatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg
     2941 ctcaacaatg ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc
     3001 atccagtttc accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg
     3061 gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc
     3121 ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag
     3181 cacccagtta aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta
     3241 caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat
     3301 cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt
     3361 gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat
     3421 ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg
     3481 gacagaggat aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac
     3541 catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg
     3601 gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca
     3661 gcaggatcta tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt
     3721 ccagactggc ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata
     3781 acgcgcagct gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg
     3841 ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg
     3901 tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact
     3961 tcaagaatgc cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga
     4021 atcataccag gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca
     4081 ccttcgctac ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg
     4141 ctgacgacgt ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt
     4201 ttttggcttt ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca
     4261 ggtgatcgta ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga
     4321 cgtatttacc cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc
     4381 gctggatctt acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac
     4441 ccaggatggc gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac
     4501 gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt
     4561 ccagcgggtc aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg
     4621 ttgcgtcaag gtgcgcaaag gtggttgccg gagacggatc agtcaagtca tccgcaggta
     4681 cgtataccgc ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca
     4741 gaacgcccat ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca
     4801 gcagtgcgga tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca
     4861 gaacgtcacg accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc
     4921 gcagacggtt tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa
     4981 cgttggagtc ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc
     5041 ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt
     5101 ttacggtttt acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg
     5161 gacacatcag gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct
     5221 cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt
     5281 tcatgtcgac cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg
     5341 ggacttcgat cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg
     5401 aacccattgc gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca
     5461 gacgctcatt accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag
     5521 ggaattcgac gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa
     5581 atcctctacg aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc
     5641 ctgagtaatg ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct
     5701 gccgccattg tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag
     5761 gttttcaacc acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag
     5821 caacgccttc ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc
     5881 atctgatgcc ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat
     5941 aaatttgttg ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat
     6001 cacttttacc ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg
     6061 ggcaacaaca ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc
     6121 gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa
     6181 caggttaatg ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac
     6241 gcgtttaacg tcgcgttctt ccaggtatgg gtgcttatat tccagattac cgctcgcaag
     6301 gtgaccaatc actttgcgca tggtatctgc ataaggacgg ctggccgcca tgcgatcctg
     6361 cgatttacgc attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt
     6421 ctggacgctt gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat
     6481 gccttgcggc ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag
     6541 gaatcgagga tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg
     6601 atctcttgca tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag
     6661 ctgccgattt tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc
     6721 agagactgtt gcgctacgga catcggcgca tactgtttct gtttcagcag ttcggtcact
     6781 ttctgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag
     6841 aacgctgcca gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc
     6901 atgatcttgg tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca
     6961 ggacgaatac cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag
     7021 attacgttgg tcggaacgaa cgcagaaacg tcacccgcct gagtttcaat aatcggcagt
     7081 gcggtcagag aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg
     7141 gcgttaacac gcgcagcacg ctccagcaga cgagagtgga ggtagaatac gtcgcccggg
     7201 aatgcttcac gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc
     7261 tgtttagaca ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg
     7321 cccattgcgc aaccggcata cggagccaga tattgcagtg cagcggattc agacgcggtt
     7381 gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta
     7441 gaaatggtgg acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc
     7501 tggttgatga tggcatcgat agccagtgcg gttttaccgg tctgacggtc accgatgatc
     7561 aattcacgct gaccacgacc aattgggatc atggagtcaa cggctttata accggtctgt
     7621 accggctgat ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag
     7681 aagccgtcgt gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca
     7741 cggcccagca ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc
     7801 atgccttcgg caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg
     7861 aggttcagtg cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa
     7921 tcggccaggc cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg
     7981 tgagcttcac tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg
     8041 gtggaattca gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca
     8101 agacgaccgc gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc
     8161 attacagact tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc
     8221 gcagcagaaa ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc
     8281 tcagcggtag cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc
     8341 gcgttaagac gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt
     8401 tgctcaccac aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag
     8461 agaagctctg ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc
     8521 cagcgttcta cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga
     8581 gctaccgtaa taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat
     8641 ccacgatgtc gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag
     8701 caacagccag gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt
     8761 caatttctgc ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg
     8821 cttcgtccag aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg
     8881 ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg
     8941 ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca
     9001 ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct
     9061 ggccgaggat tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga
     9121 tattgctctt taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc
     9181 agacctacag cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta
     9241 cgcagcagag gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg
     9301 atgccgatac cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg
     9361 tacagcagat ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt
     9421 agtagtgttg gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac
     9481 catgaagatg aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt
     9541 caggatccac tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc
     9601 ggcatacatg ttaccgaaca gtcgcaaacc aagtgaaact ggtttggaca gcaggcttac
     9661 cccttcaagg attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa
     9721 ctctttcgtg aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa
     9781 tacgcccaat gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc
     9841 aggcagaccc agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat
     9901 caggttcatc aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct
     9961 tttgccatgg tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc
    10021 ggtctgaaac ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac
    10081 caggaacaac agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa
    10141 ggtggctggg gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt
    10201 attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt
    10261 taccctttgt tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac
    10321 gtaacgatca gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc
    10381 aacatcgcca gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc
    10441 gctggtgtat gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag
    10501 actgccaggc ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc
    10561 aatccacttg ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga
    10621 ctcacgagcg acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg
    10681 tcgtataaaa ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg
    10741 tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt
    10801 tccagtgcat acaattgcga cttttctgct aaccctgttc gatcatgaaa aactgtaaat
    10861 aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac
    10921 agcatattta tttacttggc aaatgatgcc tttgcaagtt tatgata
//
LOCUS       DAZFDP010000151        10958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFDP010000151 DAZFDP010000000
VERSION     DAZFDP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..815
                     /gene="adiY"
                     /locus_tag="ACHJ1G_004077"
     CDS             54..815
                     /gene="adiY"
                     /locus_tag="ACHJ1G_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4765838.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            952..2289
                     /gene="adiC"
                     /locus_tag="ACHJ1G_004078"
     CDS             952..2289
                     /gene="adiC"
                     /locus_tag="ACHJ1G_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4765839.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2393..4036
                     /gene="eptA"
                     /locus_tag="ACHJ1G_004079"
     CDS             2393..4036
                     /gene="eptA"
                     /locus_tag="ACHJ1G_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4765840.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4033..4701
                     /gene="basR"
                     /locus_tag="ACHJ1G_004080"
     CDS             4033..4701
                     /gene="basR"
                     /locus_tag="ACHJ1G_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4765841.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHJ1G_004081"
     CDS             4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHJ1G_004081"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4765842.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHJ1G_004082"
     CDS             complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHJ1G_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4765843.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHJ1G_004083"
     CDS             complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHJ1G_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO4765844.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHJ1G_004084"
     CDS             complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHJ1G_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4765845.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHJ1G_004085"
     CDS             complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHJ1G_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO4765846.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2736 a         2849 c         2705 g         2668 t
ORIGIN      
        1 atcgctttgc tttagcgaga actggtcttt tatttgcact caggagtaca tgtatgagga
       61 tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga
      121 atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata
      181 atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca
      241 tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta
      301 gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc
      361 aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat
      421 tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt
      481 tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact
      541 ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagt
      601 tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg
      661 taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca
      721 acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt
      781 atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata
      841 ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta
      901 tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg
      961 gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt
     1021 atggggtcag gtgtttttct gttacctgca aacctggcct ctactggcgg gattgccatt
     1081 tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg
     1141 tcgttcctcg atccaagtcc tggtggttct tacgcgtacg cccgccgctg ctttggcccg
     1201 tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc
     1261 atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg
     1321 gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt
     1381 ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat ccccatcgtc
     1441 gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac
     1501 gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac gctgtggtcg
     1561 ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat
     1621 gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt actttctacc
     1681 accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc gccattcggt
     1741 gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt ctgcgcagct
     1801 gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc
     1861 gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc gggcacgcca
     1921 gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca
     1981 ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt tacactggtg
     2041 ccatatcttt acacctgtgc ggcgttactg ctgctcggtc acggtcactt tggtaaagca
     2101 cgcccggcat atctggctgt tactaccatt gccttcctct actgcatttg ggccgtggtg
     2161 gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtaat caccgccatg
     2221 tatgcgctga attacaaccg gctgcataaa aacccgtatc ccttagatgt accaataagc
     2281 aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg
     2341 cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa
     2401 gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta
     2461 tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga
     2521 ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca gcgtgattaa
     2581 tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat
     2641 tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc
     2701 gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga tgacaccgca
     2761 aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggataaa
     2821 aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt
     2881 ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt
     2941 ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg
     3001 gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg gtgaagacgc
     3061 ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg
     3121 cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg
     3181 gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc
     3241 agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag aagagctggc
     3301 acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa
     3361 tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc
     3421 gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg
     3481 gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg
     3541 cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac
     3601 ctgcgacacc aatgagatcc agacctgtag caaagagcaa ctggtgaaca cttacgacaa
     3661 cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca
     3721 ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa
     3781 tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc
     3841 gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc agaactgcct
     3901 gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg
     3961 attaactggc gttgagacga agtattacca ggctgcggat gatattctgc aaacttgcag
     4021 gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggattg
     4081 attctggcgg cgcaaactga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg
     4141 gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg gttacccgac
     4201 gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccagtactg
     4261 atcctcaccg ctcgcgatac gctgaccgac aaaatcgctg ggctggatgt cggtgccgat
     4321 gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta
     4381 cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg
     4441 ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct
     4501 ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat tctctacaac
     4561 gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat
     4621 ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg
     4681 gtcgcgaatg aggaaaacta attgaatctg atgcattttc tgcgccgacc aatatcgctg
     4741 cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc
     4801 ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac
     4861 aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt
     4921 gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc ggtgcgccgc
     4981 atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcactgc cgacaactta
     5041 acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttaaac
     5101 gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc
     5161 gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa
     5221 acgcatcaca ttgatgtagc accgttagtg gcacgacttg atcagatgat ggagagcgtc
     5281 tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat
     5341 gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac
     5401 cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat
     5461 gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg
     5521 caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat ggcagttgaa
     5581 gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt
     5641 atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag
     5701 ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg
     5761 gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc
     5821 agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc
     5881 gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc
     5941 ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt
     6001 tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg
     6061 attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct
     6121 gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg
     6181 gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc
     6241 gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata
     6301 ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta
     6361 gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa
     6421 atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagg
     6481 gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc
     6541 accggctgga caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct
     6601 tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa
     6661 gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca gtgtttggtg
     6721 gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc
     6781 agtttatcga catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc
     6841 ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag
     6901 ttcgcttcgc cgacaatggt cgaaattaac accactacgc cagcacccag cacaaacccg
     6961 gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc aggtgagtat
     7021 tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc
     7081 ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag
     7141 ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga
     7201 ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat cagaaaggga
     7261 acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa
     7321 actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca
     7381 ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca
     7441 tcacgaaggg taattggttt tacttttttc cttttcagca tagctttcct cgcagagttg
     7501 gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt
     7561 gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat
     7621 gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt
     7681 gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga
     7741 ttatttacgc caaatttaca gagaagattt cctcgagcat tgcccccgcc agacgctgcg
     7801 ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc
     7861 aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat
     7921 ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg
     7981 gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa tcacccagca
     8041 acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc ccccaacgca
     8101 actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac
     8161 gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg cctcgttgcg
     8221 ttaacgcctt ttccttccag cggcaacggg ctgaaaacca ctcatacaag gcggcaattt
     8281 gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca
     8341 gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac gccagttttt
     8401 ccaccaatgt cagacgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct
     8461 gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg
     8521 cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg ggataaaccg
     8581 actccagcac gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt ctgccgtgaa
     8641 aagtgtttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc cgtgagtcat
     8701 cacagtccgc tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag
     8761 agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgaa
     8821 aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat
     8881 ttgttggcaa taaagatcga cttctgcgct taaagctcgt tgcaacacac tgtaatcaac
     8941 cgtctctgtc ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga
     9001 aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt cctggcgcga
     9061 aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actgcgccgt gaaaaacagg
     9121 aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat
     9181 ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg caaaacgcag
     9241 cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg
     9301 aatctcgctt aacggctccg aatcatcctg aaacaacgcg gctaactgtg cattcaccgc
     9361 atcttgctgt tgacgcagaa agtggttggc ggaggccaag gccagctcga tttcatgttt
     9421 aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg
     9481 atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag
     9541 gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc gcagagcata
     9601 caacgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga
     9661 atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg
     9721 acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg
     9781 cggtggtaac ttaccgttgt tggctaactc atggcgcgcc cgattcgcca ggtagcccca
     9841 catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga
     9901 aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt
     9961 gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc
    10021 atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc
    10081 cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt
    10141 atccagtaac gtcaactgac cgggataact ttccagcccc gccagatgga caaactccac
    10201 ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag
    10261 cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc
    10321 acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat
    10381 atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg
    10441 ttgtaatttt tgaattaaac ggtcgattgg cgcgacatgt gaaaaatgca gtaccggttc
    10501 tttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt
    10561 aggcaaaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt
    10621 accgacaatc gccagcacca tttccagtcg ggaaatttta cgcaactcat tattcagcat
    10681 cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcatcgtc
    10741 tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa
    10801 caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc
    10861 cttaaagcaa attttgttac ttttatttag ccagattgtt tttgagttct gttttcggct
    10921 tttataatta ctgcaagaaa taattttata tttagcgt
//
LOCUS       DAZFDP010000152        10904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFDP010000152 DAZFDP010000000
VERSION     DAZFDP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..547
                     /locus_tag="ACHJ1G_004086"
     CDS             <1..547
                     /locus_tag="ACHJ1G_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO4765847.1"
                     /translation="WYLVYDLIEFIIERTNSYINLSKHLNSIFKKHGVGYTIINGCIT
                     PISNDNEIESVQNAVDNGTDSSRSHFERALQLMTDREQPDYRNSIKESISAIESLCRK
                     ITGNDKGTLGACLKAIEEKGYIHSAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAE
                     AKFMLVTCSAFSNYLLSKISD"
     gene            complement(596..1168)
                     /locus_tag="ACHJ1G_004087"
     CDS             complement(596..1168)
                     /locus_tag="ACHJ1G_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765848.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            1269..2439
                     /locus_tag="ACHJ1G_004088"
                     /pseudo
     CDS             1269..2439
                     /locus_tag="ACHJ1G_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2522..2779
                     /locus_tag="ACHJ1G_004089"
     CDS             2522..2779
                     /locus_tag="ACHJ1G_004089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765849.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(3337..4104)
                     /gene="fecE"
                     /locus_tag="ACHJ1G_004090"
     CDS             complement(3337..4104)
                     /gene="fecE"
                     /locus_tag="ACHJ1G_004090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO4765850.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4105..5061)
                     /gene="fecD"
                     /locus_tag="ACHJ1G_004091"
     CDS             complement(4105..5061)
                     /gene="fecD"
                     /locus_tag="ACHJ1G_004091"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO4765851.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5058..6056)
                     /gene="fecC"
                     /locus_tag="ACHJ1G_004092"
     CDS             complement(5058..6056)
                     /gene="fecC"
                     /locus_tag="ACHJ1G_004092"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO4765852.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLAMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6053..6955)
                     /gene="fecB"
                     /locus_tag="ACHJ1G_004093"
     CDS             complement(6053..6955)
                     /gene="fecB"
                     /locus_tag="ACHJ1G_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO4765853.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7000..9324)
                     /gene="fecA"
                     /locus_tag="ACHJ1G_004094"
     CDS             complement(7000..9324)
                     /gene="fecA"
                     /locus_tag="ACHJ1G_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO4765854.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9411..10364)
                     /gene="fecR"
                     /locus_tag="ACHJ1G_004095"
     CDS             complement(9411..10364)
                     /gene="fecR"
                     /locus_tag="ACHJ1G_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO4765855.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10361..10882)
                     /gene="fecI"
                     /locus_tag="ACHJ1G_004096"
     CDS             complement(10361..10882)
                     /gene="fecI"
                     /locus_tag="ACHJ1G_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO4765856.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
BASE COUNT         2518 a         2914 c         2992 g         2480 t
ORIGIN      
        1 atggtatttg gtatatgatt taattgagtt catcattgag agaactaatt catatattaa
       61 tctatcgaag catctcaatt ccatctttaa aaagcatggc gttggttata ccataataaa
      121 tggttgtatt actcctattt caaatgataa cgaaattgaa agtgttcaga acgcagttga
      181 taatggcact gattcatcgc gtagccattt cgaaagggcg ttacaactaa tgacggatag
      241 agagcagcca gactacagaa actcgattaa agagtctatc tctgcaattg aatctttatg
      301 caggaaaatc acgggcaatg ataaaggaac cttgggggct tgtcttaaag ctatagagga
      361 aaaaggatat atacactctg caatgaaagg cgcttttagt cagctctatg gttatacaag
      421 tgatcagggt ggaatacgtc atgcgctaac agaagaagat gtaaatccta ccttagctga
      481 agctaaattt atgttagtca cttgcagtgc gttcagtaat tatctgttgt caaaaataag
      541 tgattaatta caggtttata gttacgagtg ggttagttac ccactccctt taaaattatt
      601 caccagactc taatcttttt tctcttttct catctctcct ttttattgcc aaatcgtgtt
      661 gctcttgcag atacgtgcgt tcttcgtttc tatcgcttga agtatatacg tctgctttta
      721 cttttttagc tccttgtgca ataggtttcc aatcctctct tataccttta atttgttctg
      781 caatttgcgt caatgggctt ctcgctccta gataacttat tccttcaaat gtcgccatgt
      841 catacacata ttcatttgtt tctctatcgt ttgatttagg tttgcttgcc cagcttatat
      901 taatattata aatttgatct ttgatttgtt catacttaca gagattgctg gtaagtgatt
      961 gcccattttt taacacgctt accttactaa acggaatctc acttttttct ctatgctccg
     1021 catttactag tggaggatca aacacaacct caacattata ggcaggtgcg ttccctgtat
     1081 tagcaatgtt aatatcaaag taaaaagcgg cccaagggtt tggctcaagt gttacaacta
     1141 tatggggttg agctgatgcg tcaaccattc tttttgtctc gtctgctctg aagtggtgaa
     1201 gtggtttact gaatttggcc acctgaacag aggtgatatg ctcacctcag aacaacacag
     1261 gtgctccaat gaaaaaaaga aattttagcg cagagtttaa acgcgaatcc gctcaactgg
     1321 ttgttgacca gacatacacg gtggcagatg ccgccaaagc tatggatgtt ggcctttcca
     1381 caatgacaag atgggtcaaa caactgcgtg atgagcgtca gggcaaaaca ccaaaagcct
     1441 ctccgataac accagaacaa atcgaaatac gtaagctgag gaaaaagcta caacgcattg
     1501 aaatggagaa tgaaatatta aaaaaggcta caaccgcgct cttgatgtca gactccctga
     1561 acagttctcg ataatcagga aactcagagc gcattatcct gtggtcacac tctgccatgt
     1621 gttcggggtt catcgcagca gctacagata ctggaaaaac cgtcctgaaa aaccagacgg
     1681 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
     1741 cggagcaaga agcatcgcca caatggcaac ccggagaggc taccagatgg ggcactggct
     1801 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
     1861 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
     1921 aacggaacca aatcaggtgt ggtgcggtga tgtgacctat atctggacgg gtaagcgctg
     1981 ggcgtacctc gccgttgttc tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc
     2041 gttctcgccg gacagcaggc ttaccatgaa agcactggaa atggcatggg aaacccgtgg
     2101 taagcccgtc ggggtgatgt tccaggcgat caaggcagtc attatacgag caggcagttc
     2161 cggcagttac tgtggcgata ccggatcagg cagagtatga gtcggcgtgg aaactgctgg
     2221 gataacagcc caatggagcg cttcttcagg agtctgaaga acgaatgggt gccagcgacg
     2281 ggctatgtaa gcttcagcga tgcagctcac gcaataacgg actatatcgt tggatattac
     2341 agcgcactaa gaccgcacga atataatggt gggttaccac caaacgaatc agaaaaccga
     2401 tactggaaaa actctaacgc ggaggccagt tttagttgac cacaacagac tacctgaagg
     2461 gagccgcggt cgcctagcag ttgcagtagc aggagatcat ccagccgcag tacaggtcac
     2521 gatgactctg gttaatgata ccggctttga ccccgtattt tccggctcta tcgctgaatc
     2581 atggcgtcag cagccgtgca caccatccta ttgttgtgac tgggaggctg ccaccatgct
     2641 tcgcgctttc cctctggcga aaaagggaga aggacgggcc cgtctgcctt cactttatgc
     2701 cagcttcggt aagctgggtg agacaccgac tcataaagat atcattgata acaatcgatc
     2761 catcaactgg cctgtataac gtggctgccg gtgattaaga aagctgcacc tacctaagta
     2821 gtagcaaacg cacacttttt agaaaaatcg atggtcagaa actggattag caattccgtt
     2881 ccagggttgc ttttgattta cgttggcgtc tgatcattga tttatcctca aaagcccaac
     2941 ctcattggta atgaaccagc tccgtgaatg tccgctctgg cacagagcga aattttttga
     3001 tctccccccc tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac
     3061 ttcgcgtttt tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat
     3121 tgattgccgt gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg
     3181 cgttgattat gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt
     3241 tcagagtccg gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc
     3301 attcagtcgt ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct
     3361 gccagatacc ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc
     3421 tggggtcatc acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac
     3481 cagttgatcg cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt
     3541 cccctgagtc cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag
     3601 ataggtggtt ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccatcgccag
     3661 aaatgcgcgc tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt
     3721 gatccgggtc tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc
     3781 ccagagtgac agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc
     3841 tggcgttaaa tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag
     3901 catatttatg ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga
     3961 aaaacagttt aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt
     4021 ccccgttggc agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aactgaccgt
     4081 cagattttca gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg
     4141 cgcaccgata atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct
     4201 cgccagcaga tcggcaacca ccaacagcaa cgcacctgtc agggctgaaa caggcagcag
     4261 tctgcggtga cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa
     4321 gctaatcggg ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc
     4381 ccagaatcgg gtatggggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc
     4441 aaggaggtcg agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg
     4501 gatggcaatc ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag
     4561 ggcgttgttc acatcctgtg ggcgcgagag catcagataa tccgtcaggc tggcccagca
     4621 tgcagaaagc gccacgccgg tgagcgccag cttcatcggc tggtgggtct ttgccagcat
     4681 cttcagtaat atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac
     4741 gggcagtgac ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac
     4801 gccgagaata tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc
     4861 cacggcgagg gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat
     4921 caatacataa taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac
     4981 ggggatcact cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa
     5041 aataaccagc gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca
     5101 atcagcgcca gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt
     5161 acatctgcca gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc
     5221 tgatcgaagc ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc
     5281 ggacctgcca cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa
     5341 cgtagcctcg tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg
     5401 ttcagttgat tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc
     5461 tgccagacat cctgccagcg ggcgtgggac actccgcctg ccagccagta aaagatgccg
     5521 taagcatgat cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac
     5581 agcgcgatac tcgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct
     5641 gcggtcatgg ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa
     5701 tagcctgcaa tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg
     5761 ctgttaatgc cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc
     5821 gtgcccgcga gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga
     5881 aggttttgca ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt
     5941 gcatctgctc cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata
     6001 ataagtgctg caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca
     6061 caacggtaag cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag
     6121 caaaaatacc gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct
     6181 gcgcggcggt taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt
     6241 cgcgatagtg ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga
     6301 tggacggcat ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca
     6361 cgctgccggt ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa
     6421 aggccacgcg tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt
     6481 gttccaggcg tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag
     6541 attgcaaatt ttcagcgtag gtttcgttgc gggacttaag cagcagtacc ggcgcgattt
     6601 gctgcaaggc gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt
     6661 tcagagcggc aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt
     6721 tcaggtgcgc acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga
     6781 tcgggctgac gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc
     6841 gttgtggcgt tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg
     6901 cgtggctgat caccagcagc aggcctgcaa aaacaaaacg gataaatgcc aacataatca
     6961 cattccagct aaaagcccgg caagccgggc gttaacacat cagaacttca acgacccctg
     7021 catatacagc gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg
     7081 gatgaagtag tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat
     7141 ctgcgggcca aaatcatacg ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc
     7201 ggtactgccg tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg
     7261 gaaatcgctg ttcagattga acgtccagtt tcctggcttg tagtccacgc ccagcgtgcc
     7321 tttatgtttc ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc
     7381 cgcgttcaca tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt
     7441 acccagatcg taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt
     7501 gacggtgtcg ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc
     7561 catttccgcc gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt
     7621 ttccggttcc acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc
     7681 gaacgagcct tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt
     7741 caacgccgga agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt
     7801 ctggtatgac tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc
     7861 gattttgtca tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta
     7921 agggcttgag ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg
     7981 cgttgattca ttcaaatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat
     8041 aaagatctgg ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt
     8101 gatgcgtttg ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat
     8161 gttgaattta tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg
     8221 accccagaag cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg
     8281 agacaggcca ccgggcatgt cggcttcacc atcgtaatat tgcagcaggc tgttgaaggt
     8341 gtgcacctca tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct
     8401 gtgctcgcgc cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt
     8461 gtccgctgtg ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt
     8521 tgggctgagc tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt
     8581 aacaaagttc accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg
     8641 taccacgtca atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg
     8701 accgtaaggg gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag
     8761 gcgcgggctc aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt
     8821 gttttccggc gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc
     8881 ggttttggcg aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc
     8941 gttttcacgc gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc
     9001 gggcgcgggc tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc
     9061 gtccagcagt tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg
     9121 cttgccgcgc gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg
     9181 attgagcgct ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa
     9241 cgagagaccg gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt
     9301 acgaaaaacg cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat
     9361 gagtcgaacg agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt
     9421 gaaatgttta tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata
     9481 acgttcagga tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca
     9541 acggcgggat cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca
     9601 cccagcggtt tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc
     9661 agcggtttca ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg
     9721 atgcgttttt gcgcgggggc actggcgaga agcacttcca cagcgtgctg ctgcacgtca
     9781 agctgcgtga aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg
     9841 ccctgacggg tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg
     9901 atttcaccgt accagagccg gacggtgcgc tgatgcgcat caaaacgcac atccgccgcg
     9961 ctttgggtat tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct
    10021 ttggcggtgc ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag
    10081 cctccgccag cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg
    10141 gtatcgtgca acgccctgct ggctacgtcg ccaggcacac cgccaagctg gttgcgcagg
    10201 ttttcaacct gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag
    10261 cgcgcttcct gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct
    10321 gaacgcagcg cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac
    10381 ggaacagcag gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa
    10441 caccgagttt gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg
    10501 cttcacgtgt tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga
    10561 gttggctttc gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct
    10621 ccagatacgc tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga
    10681 tagtgcagag gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc
    10741 gcaaaaaagt gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca
    10801 gccagctttt caaccagccg tgatgtgtgc cataaagcga ctcgaacgtt aaggaagctg
    10861 tggtagtggc gcggtcagac atgcggagtg catcaaaagt taat
//
LOCUS       DAZFDP010000153        10851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFDP010000153 DAZFDP010000000
VERSION     DAZFDP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            275..679
                     /gene="sdhC"
                     /locus_tag="ACHJ1G_004097"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             275..679
                     /gene="sdhC"
                     /locus_tag="ACHJ1G_004097"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4765857.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            673..1020
                     /gene="sdhD"
                     /locus_tag="ACHJ1G_004098"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             673..1020
                     /gene="sdhD"
                     /locus_tag="ACHJ1G_004098"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4765858.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1020..2786
                     /gene="sdhA"
                     /locus_tag="ACHJ1G_004099"
     CDS             1020..2786
                     /gene="sdhA"
                     /locus_tag="ACHJ1G_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4765859.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2802..3518
                     /gene="sdhB"
                     /locus_tag="ACHJ1G_004100"
     CDS             2802..3518
                     /gene="sdhB"
                     /locus_tag="ACHJ1G_004100"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4765860.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3819..6620
                     /gene="sucA"
                     /locus_tag="ACHJ1G_004101"
     CDS             3819..6620
                     /gene="sucA"
                     /locus_tag="ACHJ1G_004101"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4765861.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6635..7852
                     /gene="odhB"
                     /locus_tag="ACHJ1G_004102"
     CDS             6635..7852
                     /gene="odhB"
                     /locus_tag="ACHJ1G_004102"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4765862.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7946..9112
                     /gene="sucC"
                     /locus_tag="ACHJ1G_004103"
     CDS             7946..9112
                     /gene="sucC"
                     /locus_tag="ACHJ1G_004103"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4765863.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9112..9981
                     /gene="sucD"
                     /locus_tag="ACHJ1G_004104"
     CDS             9112..9981
                     /gene="sucD"
                     /locus_tag="ACHJ1G_004104"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4765864.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10085..10807)
                     /gene="mngR"
                     /locus_tag="ACHJ1G_004105"
     CDS             complement(10085..10807)
                     /gene="mngR"
                     /locus_tag="ACHJ1G_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO4765865.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2560 a         2816 c         3001 g         2474 t
ORIGIN      
        1 ggataaaacc cgacaaacta tatgtaggtt aattgtaatg attttgtgaa cagcctatac
       61 tgccgccagg tctccggaac accctgcaat cccgagccac ccagcgttgt aacgtgtcgt
      121 tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta tagaaaggac gctgtctgac
      181 ccgcaagcag accggaggaa ggaaatcccg acgtctccag gtaacagaaa gttaacctct
      241 gtgcccgtag tccccaggga ataataagaa cagcatgtgg gcgttattca tgataagaaa
      301 tgtgaaaaaa caaagacctg ttaatctgga cctacagacc atccggttcc ccatcacggc
      361 gatagcgtcc attctccatc gcgtttccgg tgtgatcacc tttattgcag tgggtatcct
      421 gctgtggctt ctgggtacca gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat
      481 tatgggcagc ttcttcgtca aatttatcat gtggggcatc cttaccgctc tggcgtatca
      541 cgtcgtcgta ggtattcgcc acatgatgat ggattttggc tatctggaag aaacattcga
      601 agcgggtaaa cgctccgcca aaatctcctt tgttattact gtcgtgcttt cacttctcgc
      661 aggagtcctc gtatggtaag caacgcctcc gcattaggac gcaatggcgt acatgatttc
      721 atcctcgttc gcgctaccgc tatcgtcctg acgctctaca tcatttatat ggtcggtttt
      781 ttcgccacca gtggcgaact gacatatgaa gtctggatcg gtttcttcgc ctctgcgttc
      841 accaaagtgt tcaccctgct ggcgctgttt tctatcttga ttcatgcctg gatcggcatg
      901 tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc gcctgatgct gcaactggtg
      961 attgtcgttg cactggtggt ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga
     1021 tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg tgccggtggc gcaggtatgc
     1081 gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc gctgctctct aaagtcttcc
     1141 cgacccgttc ccataccgtt tctgcgcaag gcggcattac cgttgcgctg ggtaataccc
     1201 atgaagataa ctgggaatgg catatgtacg acaccgtaaa agggtcggac tatatcggtg
     1261 accaggacgc gattgaatat atgtgtaaaa ccgggccgga agcgattctg gaactcgaac
     1321 acatgggcct gccgttctcg cgtcttgatg atggtcgtat ctatcaacgt ccgtttggcg
     1381 gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac tgcggcggcg gctgaccgta
     1441 ccggtcacgc actgttgcac acgctttatc agcagaacct gaaaaaccac accaccattt
     1501 tctccgagtg gtatgcgctg gatctggtga aaaaccagga tggcgcggtg gtgggttgta
     1561 ccgcactgtg catcgaaacc ggtgaagtgg tttatttcaa agcccgcgct accgtgctgg
     1621 cgactggcgg ggcagggcgt atttatcagt ccaccactaa cgcccacatt aacactggcg
     1681 acggtgtcgg catggctatc cgcgcaggcg taccggtgca ggatatggaa atgtggcaat
     1741 tccacccgac cggcattgcc ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag
     1801 gcggttatct gctgaacaaa catggcgaac gctttatgga gcgttatgcg ccgaacgcca
     1861 aagacctggc gggccgtgat gtggtggcgc gttccatcat gattgaaatc cgtgaaggtc
     1921 gcggctgtga tggtccgtgg gggccacacg cgaaactgaa actcgatcac ctgggtaaag
     1981 aagttctcga atcccgtctg ccgggtatcc tggagctttc ccgtaccttc gctcacgtcg
     2041 atccggtgaa agagccgatt ccggttatcc caacctgtca ctacatgatg ggcggtattc
     2101 cgaccaaagt taccggtcag gcgctgactg tgaatgagaa aggcgaagat gtggttgttc
     2161 cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg
     2221 gcggcaactc gctgctggac ctggtggtct ttggtcgcgc ggcaggtctg catctgcaag
     2281 agtctatcgc cgagcagggc gcactgcgcg atgccagcga gtctgatgtt gaagtgtctc
     2341 tggatcgcct gaaccgctgg aacaataatc gtaacggtga agatccggtg gcgattcgta
     2401 aagcgctgca agaatgtatg cagcataact tctcggtctt ccgtgaaggt gatgcgatgg
     2461 cgaaagggct tgagcagttg aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg
     2521 acacttccag cgagttcaac acccagcgcg ttgagtgcct ggaactggat aacctgatgg
     2581 aaacggcgta tgcaacggct gtttctgcca acttccgtac cgaaagccgt ggcgcacata
     2641 gccgcttcga cttcccggat cgtgatgatg aaaactggct gtgccactcc ctgtatctgc
     2701 cagagtcgga atccatgacg cgccgaagcg tcaacatgga accgaaactg cgcccggcat
     2761 tcccgccgaa gattcgtact tactaatgcg gagacaggaa aatgagactc gagttttcaa
     2821 tttatcgcta taacccggat gttgatgatg ctccgcgtat gcaggattac accctggaag
     2881 cggaagaagg tcgcgacatg atgctgctgg atgcgcttat ccagctgaaa gagaaagatc
     2941 ccagcctgtc gttccgccgc tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca
     3001 tgaacggcaa gaatggtctg gcctgtatta ccccgatttc ggcactcaac cagccgggca
     3061 agaagattgt gattcgcccg ctgccaggtt taccggtgat ccgcgatttg gtggtagaca
     3121 tgggacaatt ctatgcgcaa tatgacaaaa ttaagcctta cctgttgaat aatggacaaa
     3181 atccgccagc tcgcgagcat ttacagatgc cagagcagcg cgaaaaactc gacgggctgt
     3241 atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg
     3301 ataagtttat cggcccggca ggcttgttag cggcatatcg tttcctgatc gatagccgtg
     3361 ataccgagac tgacagccgc ctcgacggtt tgagcgatgc attcagcgta ttccgctgtc
     3421 acagcatcat gaactgcgtc agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg
     3481 gccatattaa gtcgatgttg ttgcaacgta atgcgtaaac cgtaggcctg ataagacgcg
     3541 caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa
     3601 gtcattaccc gtaggcctga taagcgcagc gcatcaggcg taacaaagaa atgcaggaaa
     3661 tctttaaaaa ctgcccctga cactaagaca gtttttaaag gttccttcgc gagccactac
     3721 atagacaaga gctcgcaagt gaaccccggc acgcacatca ctgtgcgtgg tagtatccac
     3781 ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag
     3841 cctggttgga ctcttcttac ctctctggcg caaaccagag ctggatagaa cagctctatg
     3901 aagacttctt aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt
     3961 tacctggtac gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc
     4021 gcctggcgaa agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga
     4081 agcaggttaa agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga
     4141 atctcgatcc gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc
     4201 acgatctgac cgaagcagac ttccaggaga ccttcaacgt cggttcattt gccagcggca
     4261 aagaaaccat gaaactcggc gagctgctgg aagccctcaa gcaaacctac tgtggcccga
     4321 ttggtgccga gtatatgcac attaccagca ccgaagaaaa acgctggatc caacagcgta
     4381 tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa acgcttctta agcgaactga
     4441 ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt ccctggcgca aaacgcttct
     4501 cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga gatgatccgc cacgctggca
     4561 acagcggcac ccgcgaagtg gttcttggga tggcgcaccg tggtcgtctg aacgtgctgg
     4621 tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga gtttgccggt aaacataaag
     4681 aacacctcgg cacgggtgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg
     4741 atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc tcaccttgag attgtaagcc
     4801 cggtagttat cggttctgtt cgtgcccgtc tggacagact tgatgagccg agcagcaaca
     4861 aagtgctgcc aatcaccatc cacggtgacg ccgcagtgac cgggcagggc gtggttcagg
     4921 aaactctgaa catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta
     4981 tcaacaacca ggttggtttc accacctcta atccgctgga tgctcgttct acaccgtact
     5041 gtactgatat cggtaagatg gttcaggcac cgattttcca cgttaacgcg gatgatccgg
     5101 aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg
     5161 tcttcatcga cctggtgtgc taccgccgtc acggccacaa cgaagccgac gagccgagcg
     5221 caacccagcc gctgatgtat cagaaaatca aaaaacatcc gacaccgcgc aaaatctacg
     5281 ctgacaagct ggagcaggaa aaagtggcga cgctggaaga tgccaccgag atggttaacc
     5341 tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc agagtggcgt ccgatgaaca
     5401 tgcactcttt cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaaca
     5461 aagttgagat gaagcgcctg caggagctgg cgaaacgcat cagcacggtg ccggaagcag
     5521 ttgaaatgca gtctcgcgtt gccaagattt atggcgatcg ccaggcgatg gctgccggtg
     5581 agaaactgtt cgactggggc ggtgcggaaa acctcgctta cgccacgctg gttgacgaag
     5641 gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg
     5701 cggtgatcca caaccagtct aacggttcca cttacacgcc gttgcaacac atccataacg
     5761 ggcagggcgc gttccgcgtc tgggactccg tactgtctga agaagcagtg ctggcgtttg
     5821 aatacggtta tgccaccgca gaaccacgca ctctgaccat ctgggaagcg cagttcggtg
     5881 acttcgccaa cggtgcgcag gtggttatcg accagttcat ctcttctggc gaacagaaat
     5941 ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg
     6001 agcactcctc cgcgcgtctg gaacgttatc tgcaactttg tgctgagcaa aacatgcagg
     6061 tgtgcgtacc gtctaccccg gcacaggttt accacatgct gcgtcgtcag gcgctgcgcg
     6121 ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg
     6181 tttccagcct cgaagaactg gcgaacggca ccttcctgcc agccatcggt gaaatcgacg
     6241 agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgacc
     6301 tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac
     6361 tctacccgtt cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg
     6421 attttgtctg gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc
     6481 atttccgtga agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg
     6541 cctctccggc ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg
     6601 acgcgctgaa cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc
     6661 tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggcga
     6721 cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt
     6781 accggcatca gcagacggca ttctggatgc ggttctggaa gatgaaggta caacggtaac
     6841 gtctcgtcag atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc
     6901 caaatctgaa gagaaagcgt ccactccggc gcaacgccag caggcatctc tggaagagca
     6961 aaacaacgat gcgttaagcc cggcgatccg tcgcctgctg gctgaacaca atctcgacgc
     7021 cagcgccatt aaaggcaccg gtgtgggtgg tcgtctgact cgtgaagatg tggaaaaaca
     7081 tctggcgaaa gccccggcga aagagtctgc tccggcagcg gctgctccgg cggcgcaacc
     7141 ggctctggct gcacgtagtg aaaaacgtgt cccgatgact cgcctgcgta agcgtgtggc
     7201 agagcgtctg ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa
     7261 catgaagccg attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg
     7321 catccgtctg ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta
     7381 cccggaagtg aacgcgtcta tcgacggcga tgacgtggtt taccacaact atttcgacgt
     7441 cagcatggcg gtttctacgc cgcgcggcct ggtgacaccg gtactgcgtg atgtcgatac
     7501 cctcggcatg gcagacatcg agaagaaaat caaagagctg gcagtcaaag gccgtgacgg
     7561 caagctgacc gttgaagatc tgaccggtgg taacttcacc atcaccaacg gtggtgtgtt
     7621 cggttccctg atgtctacgc cgatcatcaa cccgccgcag agcgcaattc tgggtatgca
     7681 cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt gagatcctgc cgatgatgta
     7741 cctggcgctg tcctacgatc accgtctgat cgatggtcgc gaatccgtgg gcttcctggt
     7801 aacgatcaaa gagttgctgg aagatccgac gcgtctgctg ctggacgtgt agtagtttaa
     7861 gcttcacctg cactgtagac cgacatggat agggtgtcgg tctacggttt aaaagataac
     7921 gattactgaa ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactttt
     7981 tgcccgctat ggcttaccag caccggtggg ttatgcctgt actactccgc gcgaagcaga
     8041 agaagccgct tcaaaaatcg gtgccggccc gtgggtagtg aaatgtcagg ttcacgctgg
     8101 tggccgcggt aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt
     8161 tgcagaaaac tggctcggca agcgtctggt aacgtatcaa acagatgcca atggccaacc
     8221 ggttaaccag attctggttg aagcagcgac cgatatcgct aaagagctgt atctcggtgc
     8281 cgttgtagac cgtagttccc gtcgtgtggt ctttatggcc tccaccgaag gcggcgtgga
     8341 aatcgaaaaa gtggcggaag aaactccgca cctgatccac aaagttgcgc ttgatccgct
     8401 gactggcccg atgccgtatc agggacgcga gctggcgttc aaactgggtc tggaaggtaa
     8461 actggttcag cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga
     8521 cctggcgttg atcgaaatca acccgctggt catcaccaaa cagggcgatc tgatttgcct
     8581 cgacggcaaa ctgggcgctg acggcaacgc actgttccgc cagcctgatc tgcgcgaaat
     8641 gcgtgaccag tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta
     8701 cgttgcgctg gacggtaaca tcggttgtat ggttaacggc gcaggtctgg cgatgggtac
     8761 gatggacatc gttaaactgc acggcggcga accggctaac ttccttgacg ttggcggcgg
     8821 cgcaaccaaa gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa
     8881 agccgttctg gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat
     8941 catcggcgcg gtagcagaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa
     9001 caacgccgaa ctcggcgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa
     9061 aggtctgacg gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt
     9121 ttaattgata aaaacaccaa ggttatctgc cagggcttta ctggtagcca ggggactttc
     9181 cactcagaac aggccattgc atacggcact aaaatggttg gcggcgtaac cccaggtaaa
     9241 ggcggtacta cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct
     9301 actggcgcta ccgcttctgt tatctacgta ccagcaccgt tctgcaaaga ctccattctg
     9361 gaagctattg atgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg
     9421 gatatgctga ctgtgaaagt gaaactggac gaagccggcg ttcgtatgat cggcccgaac
     9481 tgcccgggcg ttatcactcc gggtgaatgc aaaatcggta tccagccggg tcacattcac
     9541 aaaccgggta aagtgggtat cgtttcccgt tccggtacac tgacctatga agcggttaaa
     9601 cagaccacgg attacggttt cggtcagtcg acctgcgttg gtatcggcgg tgacccgatc
     9661 cctggctcta actttatcga cattctcgaa atgttcgaaa aagatccgca gaccgaagcg
     9721 atcgtaatga tcggtgagat cggcggtagc gctgaagaag aagcggcagc gtacatcaaa
     9781 gagcatgtta ccaagccagt tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa
     9841 cgtatgggcc acgcgggtgc catcattgcc ggtgggaaag ggactgcgga tgagaaattc
     9901 gctgctctgg aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca
     9961 ctgaaaactg ttctgaaata aatatctgta ataagaaata gccctcgccg cttccctcta
    10021 caggaatggc gaagggctgt cggtttcgac atggttggcc atcgtatgat ggcctttttt
    10081 gtgcttatcg cgatgatttt cgttgcgcta tcagggtaaa tttatagtca tcggtattaa
    10141 aagcgttgcg gctatattca aacacccgac catcaactaa atatccacgc gatacttttt
    10201 caagaatcgg ctttgtctgg ctgatattaa gcagacggct catctcttcg gttggcatca
    10261 gaggaatgat ttcctgttcg ctacgatcga taaccatttt cttcacttct tcaataaagt
    10321 gatattttga attttccatg acctgccagg tgagatccgg gaacaacgca agcggcatcc
    10381 aggtttcttc cagcgccatt ggcttttgct tgcgatagcg cacgcgcttc acatgccaca
    10441 cacgatcctg cgcggtgatt tgtagctgtt gctgaagaaa atcgtcagcc ggaatcactt
    10501 caaatatcag gacttcactg tgtgtatcga cgtgacggtc cgacagtttt tcatcaaaac
    10561 tggttaactg aaaaatatcg taattgaccc gctcttcttt gacgtaagtc ccgctgccct
    10621 gaatgctttc gaggatctgt tgctcgacta actggcgcaa agcctgacgc accgtaaccc
    10681 ggctgacgcc aaactctgtt tgtaatgccg attcggtagg taacgcatcg ccaggtttaa
    10741 gctcgccacg cgcaatttgt tcacgaatgc gatcggcaat ctgccggtat aagggcttgt
    10801 gtcccatttt tagtatctca ttaatacgaa tttagccatt atgcccgata a
//
LOCUS       DAZFDP010000154        10692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFDP010000154 DAZFDP010000000
VERSION     DAZFDP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..818
                     /locus_tag="ACHJ1G_004106"
     CDS             93..818
                     /locus_tag="ACHJ1G_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4765866.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            776..1453
                     /gene="bglJ"
                     /locus_tag="ACHJ1G_004107"
     CDS             776..1453
                     /gene="bglJ"
                     /locus_tag="ACHJ1G_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4765867.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1491..2279)
                     /gene="fhuF"
                     /locus_tag="ACHJ1G_004108"
     CDS             complement(1491..2279)
                     /gene="fhuF"
                     /locus_tag="ACHJ1G_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4765868.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2420..2656
                     /locus_tag="ACHJ1G_004109"
     CDS             2420..2656
                     /locus_tag="ACHJ1G_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4765869.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            complement(2695..2781)
                     /locus_tag="ACHJ1G_004110"
     tRNA            complement(2695..2781)
                     /locus_tag="ACHJ1G_004110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2745..2747),aa:Leu,seq:cag)
     gene            complement(3082..4113)
                     /gene="rsmC"
                     /locus_tag="ACHJ1G_004111"
     CDS             complement(3082..4113)
                     /gene="rsmC"
                     /locus_tag="ACHJ1G_004111"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4765870.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4216..4629
                     /gene="holD"
                     /locus_tag="ACHJ1G_004112"
     CDS             4216..4629
                     /gene="holD"
                     /locus_tag="ACHJ1G_004112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4765871.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            4598..5044
                     /gene="rimI"
                     /locus_tag="ACHJ1G_004113"
     CDS             4598..5044
                     /gene="rimI"
                     /locus_tag="ACHJ1G_004113"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4765872.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5059..5736
                     /gene="yjjG"
                     /locus_tag="ACHJ1G_004114"
     CDS             5059..5736
                     /gene="yjjG"
                     /locus_tag="ACHJ1G_004114"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4765873.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5827..7416
                     /gene="prfC"
                     /locus_tag="ACHJ1G_004115"
     CDS             5827..7416
                     /gene="prfC"
                     /locus_tag="ACHJ1G_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4765874.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7809..8414
                     /gene="osmY"
                     /locus_tag="ACHJ1G_004116"
     CDS             7809..8414
                     /gene="osmY"
                     /locus_tag="ACHJ1G_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4765875.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8541..8702
                     /locus_tag="ACHJ1G_004117"
     CDS             8541..8702
                     /locus_tag="ACHJ1G_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4765876.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8824..9897
                     /locus_tag="ACHJ1G_004118"
     CDS             8824..9897
                     /locus_tag="ACHJ1G_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4765877.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9894..10676
                     /locus_tag="ACHJ1G_004119"
     CDS             9894..10676
                     /locus_tag="ACHJ1G_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4765878.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2624 a         2666 c         2845 g         2557 t
ORIGIN      
        1 ttcgcttaat ctattaattt gctggaaaca tttaaggaat gaatatcctg aataaaccat
       61 cataattttt agacagtgga tgtggaggga atatgttgcc aggatgctgc aaaaatggaa
      121 ttgttatcag taaaatacct gttatgcaag cagggttaaa agaggtcatg aggactcact
      181 tccctgaata tgaaataata tccagcgcct ctgcggagga ccttacctta ttacaattac
      241 gccgttccgg attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt
      301 gtgaacatta ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt
      361 cacgctcctg gtattcccag gcagtagaac tgctcatgtg ccctacggcg acgttattgt
      421 ctgatgttga acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag
      481 agcgtatcag cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc
      541 gctccgtcat tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg
      601 gcatcaacca gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa
      661 acagtgcgat gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata
      721 gcgcgcaggg tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga
      781 acacagccga attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat
      841 cggtattgag agtttattca gaaagtttgc gggtaaccct tataagctcc atacctatac
      901 cagtcaggag tcatttcagg atgccatgac gcggatctcg tttgcggcgg tcattttttc
      961 tttttctgcc atgagaagtg agcgcagaga gggattatct tgcctgactg aactggcgat
     1021 taagcttccg cgtacccggc gtttagttat tgcggatgat gatattgaag ctcggctgat
     1081 tggttcattg tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat
     1141 ttttcatcag gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa
     1201 caatcagtgg tacattaacc aaagccggac gctaagcccg acggagagag aaatattgcg
     1261 ctttatgtcg cgtggttact caatgacaca aattgccgag cagcttaaac gtaatatcaa
     1321 aacgatccgt gcacataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg
     1381 gttgttggag gccgcagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt
     1441 gcatccctat taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc
     1501 gtacaatcgc cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc
     1561 accagcaggc cgtcgcgcag taccacggta cgccagagtg gattatcttc accggtagta
     1621 agcgttttct caaaaaagag ggcatggcgc agcgattcaa ccgtcgcctc gccgagcagc
     1681 tgtttcatct cagtcagata ccagttgatc aaataaccgg tattactcca gataagtttg
     1741 ccgttgatct ctcccgtcgc ttctagtgcc tgcacaaccg gaaccagcgc ctggctgatt
     1801 aacgtttcca ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg
     1861 acccagaaac aggcggcacg gccggtttcg tgaaactcaa cgtggaaatg ttccggcgac
     1921 acatctaacg ctttttcctg cgtcagcagc gccagcatta atggtggcac catcaggccg
     1981 atataccatt gcgcccatag tgagatcagc ggcttgttct cgcggatcat cgttggttgg
     2041 ttgcgataga tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac
     2101 cattgcgcca gcgtcatggc gcgaagcggg gcgggttcat ccagacggat aaactccagc
     2161 aaatgttcac gatgttcggc gatcgtcgcc cgtacggctt gtgcaagccc agcatcctgc
     2221 ggctggagat gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata
     2281 atcgggatag taatctaaat gataatgatt gctaatccta gcgataggtt tacccgatag
     2341 caagggattt ctctggcttg caaatgataa aaattatcat atgatattgg ttatcattat
     2401 caatgaaaga gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct
     2461 gccgggattg ctgattgcag ggctgatgta tgcggattta tcgccggatc aatggcggat
     2521 tgtcattctg atgggattga tattgacgcc ggtgatgctg tatcacaaac agttacggca
     2581 ttacgttttg ctaccatcgt gcctggcgct tattgctggc atcatgctga tgctaatgaa
     2641 tttgaatcag ggatgaaaaa tcaaggaaga aacaagaaag gaagtaaaga taattggtgc
     2701 gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc
     2761 taccaattcc gccaccttcg cataccatca attcttaaaa agaattgcta ccacggaggc
     2821 gcattctagt ggttttcagc ttttcgtcaa tagttaatta tcgccggagg tgtaattgct
     2881 ggaaaaatgt ccatcaggaa actaacgtgc aggttttgta ggtcggataa ggcgttcacg
     2941 ccgcatccga cacggtattt gggtgcagat gccagatgcg acgcgggcgc gtcttatctg
     3001 gcctacgaag ggctaacgtg caggttttgt aggttgtata aggcgagcac gccgcatccg
     3061 acaaggcttg cggcgggata cttaaccttt cttcgcctgg cgggtcataa tggcgcgata
     3121 caccttgaag cgcccggttt gcgcgataac ttcatggaag ccaaatgtct catccagcac
     3181 gtccgggtaa ggcaggaagg cgttcgctac aattcgcagc tcgccgccgc tattaagatg
     3241 acgcaccgcg ccgcgaatca gcgtttgcgc cgcatccagg ctggtttgca tcccatcgtg
     3301 gaacggcggg ttggagatga tcatatcaaa acgacctttc acctcggaaa agacgttgct
     3361 ggcaaagact tcaccttcaa caccgttggc cgcaagcgtc gcacggctgg cttctaccgc
     3421 cggggcagag acatcgcaca aggtgagacg aattttcggc gaatggcgcg caaaggcaac
     3481 tgaaagcaca cccgcgccac agccgacatc cagcacttta cctttcgtgt gcggggttaa
     3541 cgtcgagagc agcaactggc taccgacatc cagaccgtcg cggctaaaca cgccaggcag
     3601 cgttttgacc gtcaggccat cgacgctgta ttcgccccag aatttatccg catcaaatac
     3661 tggctgtttt tccagacgac caaaatagag gccacagcga cgagcgctgt cgactttatt
     3721 caacggcgca taatctgcca gcatctgctc ggcgctgcgc acgccgctgc ggttctcgcc
     3781 aacgacaaaa atatccgtcc ccaccggcag cagagaaagt aaattcatca actggaactg
     3841 ggcttccggt ttgttcttcg gccagtagta aatcagcgta tcgcaatctg cgacgtcatc
     3901 cgccttggcg accagactaa aacgggcgtt atcccccatc tggcggctta atacctgcca
     3961 gtggtggaat tgctgggtat gagcacggct ggccgcggta tctaaacgcg cgggcaggtc
     4021 atcctgtaag tctccggcaa acagaatacg gctttgttcg aaatcatcac tgtgacgcag
     4081 caagacttca cttgccgggg taaatgcaga catggaatgc tcctcaattg atactggcgg
     4141 cgattatagc catatgttgg cgcggtatcg acgaatttgc tatatttgcg cccctgacaa
     4201 caggagcgat tcgctatgac atcccgacga gactggcagt tacagcaact gggcattacc
     4261 cagtggtcgc tgcgtcgccc tggcgcgttg cagggggaaa ttgccattgc gatcccggca
     4321 cacgtccgtc tggtgatggt ggcaaacgat cttcccgccc tgactgatcc tttagtgagc
     4381 gatgttctgc gcgcattaac cgtcagcccc gatcaagtgc tgcaactgac gccagaaaaa
     4441 atcgcgatgc tgccgcaagg cagtcgctgc aacagctggc ggttgggtac tgacgaaccg
     4501 ctatcactgg aaggcgctca ggtggcatca ccggcgctca ccgatttacg ggcaaaccca
     4561 acggcacgcg ccgcgttatg gcaacaaatt tgcacatatg aacacgattt cttccctcga
     4621 aacgactgat ttaccagcgg cttaccacat tgaacaacgc gcccacgcct ttccgtggag
     4681 tgaaaaaacc tttgccagca accagggcga gcgttatctc aactttcagt taacgcaaaa
     4741 cggcaaaatg gcggcgtttg cgattacgca agtggtgctg gatgaagcta cattgttcaa
     4801 tattgcggtc gatcctgact atcagcgtca gggattggga agggcgctgc tggaacatct
     4861 gatcgacgaa ctggaaaaac gcggcgtggc gacgctctgg ctggaagtcc gtgcctcaaa
     4921 tgctgccgcc attgccctgt acgaaagttt aggctttaac gaggcgacga ttcgccgcaa
     4981 ttactacccc accacggacg gtcgcgaaga cgccatcatc atggcgttgc caatcagtat
     5041 gtaagacaag gtggaataat gaagtgggac tggattttct ttgatgccga tgaaacgctg
     5101 tttacctttg actcattcac cggcctgcag cggatgtttc ttgattacag cgtcaccttt
     5161 accgctgaag attttcagga ctatcaggcc gttaacaagc cactgtgggt ggattatcaa
     5221 aacggcgcga tcacttcatt acagcttcag cacgggcgtt tcgagagctg ggccgaacgg
     5281 ctgaaagttg aagcaggctt gcttaacgat gcctttatta atgcgatggc ggaaatctgc
     5341 acgccgctgc cgggcgcggt ttctctgctt aacgccattc gtggcaacgc caaaatcggc
     5401 atcatcacca acggctttag cgccttgcag caagtgcgtc tggaacgcac gggcctgcgt
     5461 gattacttcg atttgctggt gatttccgaa gaagttggcg ttgctaaacc gaataagaaa
     5521 attttcgatt atgcgctgga acaggcgggc aatcctgacc gttcacgcgt gctgatggtt
     5581 ggcgacactg ccgagtccga tattctcggt ggcatcaacg ccgggcttgc gacctgctgg
     5641 ctgaatgcgc acaatcgcga gcaaccagaa ggcatcgcgc ccacctggac cgtttcttcg
     5701 ttgcacgaac tggagcagct cctgtgtaaa cactgattgc ctcccccccg ttgatgggta
     5761 aaatagccgc aatttttcgt tttcaacaag cgcggcgcga tgccgcttac tcaagaagaa
     5821 agaattatga cgttgtctcc ttatttgcaa gaggtggcta agcgccgcac ttttgccatt
     5881 atttctcacc cggacgccgg taagactacc atcaccgaga aggtgctgct gttcggacag
     5941 gccattcaga ccgccggtac agtaaaaggc cgtggttcca accagcacgc taagtcggac
     6001 tggatggaga tggaaaagca gcgtgggatc tccattacta cgtctgtgat gcagtttccg
     6061 tatcacgatt gcctggttaa cctgctagac accccggggc acgaagactt ctcggaagat
     6121 acctatcgta ccctgacggc ggtggactgc tgcctgatgg ttatcgacgc cgcaaaaggt
     6181 gttgaagatc gtacccgtaa gctgatggaa gttacccgtc tgcgcgacac gccgatcctc
     6241 acctttatga acaaacttga ccgtgatatc cgcgacccga tggaactgct cgatgaagtt
     6301 gagaacgagc tgaaaatcgg ctgcgcaccg atcacctggc cgattggctg cggcaagctg
     6361 tttaaaggcg tttaccacct ttataaagac gaaacctatc tctatcagag cggtaaaggt
     6421 cacaccattc aggaagtccg cattgttaaa gggctgaata acccggatct cgatgctgcg
     6481 gttggtgaag atctggcgca gcagctgcgt gacgaactgg aactggtgaa aggcgcgtct
     6541 aacgagttcg acaaagaatt gttccttgcg ggcgaaatca ctccggtatt cttcggtact
     6601 gcgctgggta acttcggtgt cgatcatatg ctggatggcc tggtggagtg ggctcctgcg
     6661 ccgatgccgc gtcagactga tacccgtacc gtagaagcga gcgaagacaa atttaccggc
     6721 ttcgtattta aaattcaggc caacatggac ccgaaacacc gcgaccgcgt ggcgtttatg
     6781 cgcgtggtat ccggtaaata tgaaaaaggc atgaagctgc gccaggtgcg caccgcgaaa
     6841 gacgtggtga tctctgacgc gctgaccttt atggcgggtg accgttcgca cgttgaagaa
     6901 gcgtatcctg gcgatatcct cggcctgcac aaccacggca ccattcagat cggcgacacc
     6961 tttacccagg gtgagatgat gaagttcacc ggtattccga acttcgcacc agaactgttc
     7021 cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc tgctcaaagg gctggtacag
     7081 ctttccgaag agggcgcggt gcaggtgttc cgtccgatct ccaacaacga tctgatcgtt
     7141 ggtgcagttg gtgtgctgca gtttgatgta gtggtatcgc gcctgaagag cgaatacaac
     7201 gttgaagcag tatatgaatc agtcaacgtt gccaccgccc gctgggtaga atgtgcagac
     7261 gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc aactggcgct tgatggcggc
     7321 gataacctcg cttacatcgc taccagcatg gtcaacctgc gcctggcgca ggaacgttat
     7381 ccggacgttc agttccacca gacccgcgag cattaattct tgcctccagg gcgcggtagc
     7441 cgctgcgccc tgtcaatttc ccttccttat tagccgctta cggaatgttc ttaaaacatt
     7501 cacttttgct tatgttttcg ctgatatccc gagcggtttc aaaattgtga tctatattta
     7561 acaaagtaat gacatttctg acggcgttaa ataccgttca atgcgtagat atcagtatct
     7621 aaagccgtcg attgtcattc taccgatatt aataactgat tcagaggctg taatggtcgt
     7681 tattcatcac ccatcgcttt tgtgatggcg accattgact tctgtagagg gtgaagtctc
     7741 tccctattca gcaatgcaac ctcgtgttgc caggctcaaa ttacgagcaa acatacagga
     7801 ataaatcgat gactatgaca agactgaaga tttcgaaaac tctgctggct gtaatgttga
     7861 cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa cgcacagact accaatgaaa
     7921 gcgcagggca aaaagtcgat agctctatga ataaagtcgg taatttcatg gatgacagcg
     7981 ccatcaccgc gaaagtgaag gcggcgctgg tggatcatga caacatcaag agcaccgata
     8041 tctctgtcaa aaccgatcaa aaagtcgtga ccctgagcgg tttcgttgaa agccaggctc
     8101 aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg ggtgacctct gtcagcgaca
     8161 aactgcacgt tcgcgacgct aaagaaggct ctgtgaaggg ctacgcgggt gacaccgcca
     8221 ccaccagtga aatcaaagcc aaactgctgg cggacgatat cgtcccttcc cgtcatgtga
     8281 aagttgaaac caccgacggc gtggttcagc tctccggtac cgtcgattcc caggcacaga
     8341 gcgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt gaaaagcgtt aaaaatgatc
     8401 tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact ttgtcgggag gcgcgatgtg
     8461 caccacacta aaaatatcgc attgagtgat tttcacgctc atatcaagcg gtgacattaa
     8521 ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat atttctggtt atcgcgttaa
     8581 tcgccgccgc acttgggttt ggtggtctgg ccggtaccgc tgcaggcgca gctaaaattg
     8641 tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt catgggccga aaacgaccct
     8701 agatttcaga ccattaagaa aattttatcc aaagccagtc cagcggactg gcttttgcgg
     8761 ttttagcgaa taataaattg cgttactttg tcattctttc acaacaaaaa caggaaggca
     8821 gaggtggggc agcgaatacc tgtaacgctt ggtaatattg cgccgttgtc gctaaggccg
     8881 ttccagcctg gacgaatagc tctggtgtgc gaaggcggcg gacagcgtgg aattttcacg
     8941 gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc ctttcgatct ttatctcggc
     9001 acatctgccg gggcgcagaa cctctcggcg tttatctgca atcagcccgg ttacgcgcgc
     9061 aaagtcatca tgcgctatac cacaaaacgc gaatttttcg atccattgcg ctttgtccgt
     9121 ggaggaaatc ttatcgatct cgactggctg gtggaggcca ccgcaagcca gatgccgttg
     9181 caaatggata ccgccgcgcg gttgtttgac agcggcaaat cgttttatat gtgcgcctgt
     9241 cgtcaggatg actacgcgcc taattacttt ttaccaacca aacaaaactg gctggatgtg
     9301 attcgcgcct ccagtgcgat acctggcttt tatcgtagcg gagtgtcgct ggaaggcatt
     9361 aactacctgg atggcgggat cagtgatgcg attccggtta aagaggcggc aaggcagggc
     9421 gctaaaacgt tggtcgtcat ccgcactgtg ccgtcacaaa tgtactacac gccgcagtgg
     9481 ttcaaacgca tggaacgctg gctgggtgac agtagcctgc agccgctggt caatctggtg
     9541 cagcatcatg aaaccagcta tcgtgacatt cagcaattta ttgagaaacc accgggcaag
     9601 ctgcggatat tcgaaattta tccaccgaaa ccattacata gtatcgcgct tggcagtcgg
     9661 attccggcgc tgcgtgaaga ctataaactt gggcgtttat gcggtcgtta tttcctcgcc
     9721 acggttggca agctattaac tgaaaaagcg ccgcttaccc gccatctggt gccagtggtg
     9781 acgccggaat cgatcgttat tccgcctgcg ccagtcgcca acgatacgct ggttgccgaa
     9841 gtgagcgatg ctccgcaggc gaacgacccg acatttaaca atgaggatct ggcttgattt
     9901 gccgttttat cgatacccac tgccattttg atttcccgcc gtttagtggc gatgaagagg
     9961 ccagcctgca acgcgcggca caagcgggcg taggcaagat cattgttccg gcgacagagg
    10021 cggaaaattt tgcccgcgtg caggcgttag cggaaaaata tcaaccgctg tatgccgcat
    10081 tggggttgca tccggggatg ttggaaaaac atagcgatgt gtctcttgat cagctacagc
    10141 aggcgctgga aaggcgtccg gcgaaggtgg tggcggtggg ggagatcggt ctggatctct
    10201 tcggcgacga tccgcaattt gagaggcagc agtggttact cgacgaacag ctgaaactgg
    10261 cgaaacgcta cgatctgccg gtgatccttc attcccggcg tacacacgac aaactggcga
    10321 tgcatcttaa acgtcacgat ttaccgtgca ctggcgtggt tcacggtttt tccggcagcc
    10381 tgcaacaggc cgagcggttt gtacagctgg gctacaaaat cggcgtaggc ggtactatca
    10441 cctatccacg cgccagtaaa acccgcgatg tcatcgcaaa attaccgctg gcatcgttgt
    10501 tgctggaaac cgacgcgccg gatatgccgc tcaacggctt tcaggggcag cctaaccgac
    10561 cggaacaggc tgtgcgggtg tttgacgtgc tttgcgagtt gcgcccggaa ccagaagatg
    10621 agattgccga agtgttgctt aataatacgt atgcgttgtt tagcgttagt gggtagttta
    10681 cccttcgtag gc
//
LOCUS       DAZFDP010000155        10508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFDP010000155 DAZFDP010000000
VERSION     DAZFDP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(27..239)
                     /gene="yncH"
                     /locus_tag="ACHJ1G_004120"
     CDS             complement(27..239)
                     /gene="yncH"
                     /locus_tag="ACHJ1G_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO4765879.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(315..932)
                     /gene="yncG"
                     /locus_tag="ACHJ1G_004121"
     CDS             complement(315..932)
                     /gene="yncG"
                     /locus_tag="ACHJ1G_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4765880.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1198..2697
                     /gene="ansP"
                     /locus_tag="ACHJ1G_004122"
     CDS             1198..2697
                     /gene="ansP"
                     /locus_tag="ACHJ1G_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4765881.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2812..3873)
                     /gene="yncE"
                     /locus_tag="ACHJ1G_004123"
     CDS             complement(2812..3873)
                     /gene="yncE"
                     /locus_tag="ACHJ1G_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4765882.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4115..6217
                     /gene="pqqU"
                     /locus_tag="ACHJ1G_004124"
     CDS             4115..6217
                     /gene="pqqU"
                     /locus_tag="ACHJ1G_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4765883.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6253..6918)
                     /gene="mcbR"
                     /locus_tag="ACHJ1G_004125"
     CDS             complement(6253..6918)
                     /gene="mcbR"
                     /locus_tag="ACHJ1G_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4765884.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7116..8153)
                     /gene="curA"
                     /locus_tag="ACHJ1G_004126"
     CDS             complement(7116..8153)
                     /gene="curA"
                     /locus_tag="ACHJ1G_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4765885.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVLVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8334..8852
                     /gene="mddA"
                     /locus_tag="ACHJ1G_004127"
     CDS             8334..8852
                     /gene="mddA"
                     /locus_tag="ACHJ1G_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4765886.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8849..9298
                     /gene="ydcZ"
                     /locus_tag="ACHJ1G_004128"
     CDS             8849..9298
                     /gene="ydcZ"
                     /locus_tag="ACHJ1G_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4765887.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9299..9532)
                     /gene="ydcY"
                     /locus_tag="ACHJ1G_004129"
     CDS             complement(9299..9532)
                     /gene="ydcY"
                     /locus_tag="ACHJ1G_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4765888.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9618..9791)
                     /gene="ortT"
                     /locus_tag="ACHJ1G_004130"
     CDS             complement(9618..9791)
                     /gene="ortT"
                     /locus_tag="ACHJ1G_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4765889.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9986..10081
                     /gene="yncL"
                     /locus_tag="ACHJ1G_004131"
     CDS             9986..10081
                     /gene="yncL"
                     /locus_tag="ACHJ1G_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4765890.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2588 a         2538 c         2524 g         2858 t
ORIGIN      
        1 aaatacagag aacgccattt atccttttag catacctgcc ggatattttt atttgcatta
       61 atattaattg catctccaac tccgagcata tccatcatta aaaacttttt gagattatag
      121 aatacctgtg ataacaagtg aaacatctgc acaatgtgaa tagttagttt cttttcagag
      181 gaaatgaaat agacaagctg aatgaagggg agagtgatat aaatcaaaaa acataacatg
      241 aatttaatta attacaccca tcattgtgga taaagttact gaataataaa taacataatc
      301 ttctggtatc ccactcaaat aatttcattc ctttttaata cttcttgtaa tttcggtaac
      361 tgacatacag catcagcgat cgcgctgata ttctgcgcat tatcctgaaa ccattcatgc
      421 cccggccccc atgtgcgcat ggtgcagaga taacagtcca ccagcgttag ttgctcacca
      481 aaggcataag gttcagcggt caattgtgaa ttcagccaga tataaagtga tttgcgatat
      541 tcaattacat tcttctttaa ctgttcaggt gcgtcagatg cccaacgctc agggtaatcg
      601 gcgaaagtga atgttggata gacattagca accagccaga ccaatagccg ttgaaactgc
      661 tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc gacgatcgag aaccataaat
      721 gcaatcgccg ctgtctccgt cataatctcg tcattttcca gcgccagtgt cggaacctga
      781 cacaacgggt tcagggtttt caataactcg cgtgaagctc cctcgtggtc aaaaccactg
      841 acatcaacga actgataagg aatatcagcc agtgttagca tcagctcact gattgtagag
      901 ccccatccgg gtacgccata caccttaatc atatgccctc acaatctttt aaagattaag
      961 tgtaggacag tacacaggtt aaccagcaaa aagatttgtc attttttcgt cagttgtatt
     1021 attccccttc aggattgtaa ggggatgttt tgcatcgttt atcgataacg ggtaggatgc
     1081 tgcgccgata gttttatacc cataaagaat aatggtgata actatcatcg ccaggatgaa
     1141 taaacattgt tcatggcaac ttatatgact ttttcattaa agcaatcagg gagagcaatg
     1201 agtaaacacg acaccgacac ttcagatcaa cacgccgcga aacgccgctg gcttaatgcc
     1261 cacgaagagg ggtatcacaa agcgatgggc aatcgccagg tacagatgat cgccattggc
     1321 ggcgcgattg gcaccggctt gtttttaggt gcaggagccc gactgcaaat ggcgggtcca
     1381 gcattggcac tggtttattt aatttgtggc ttgttttcgt tttttattct gcgtgcattg
     1441 ggtgagctgg tgctacaccg cccttccagt ggcagttttg tctcttatgc ccgtgagttt
     1501 ttgggtgaga aagccgctta tgttgctggc tggatgtact tcatcaactg ggcgatgacg
     1561 gggattgttg atattaccgc cgtcgctctg tatatgcatt actggggtgc gtttggcggc
     1621 gtgccgcagt gggtctttgc gctcgctgca cttaccatcg ttggcaccat gaatatgatc
     1681 ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc ttattaaagt gctcgccatt
     1741 gtgacctttt tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct ggatggcaac
     1801 accactggct ttcatttaat taccgataat ggcggcttct tcccccacgg tttgctgcct
     1861 gctctggtgt tgattcaggg cgtagtgttt gcttttgcct ccattgaaat ggtgggtaca
     1921 gctgccggag aatgtaaaga tccgcagacc atggtgccta aagcgattaa cagcgtgatt
     1981 tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc tggttatgct attgccgtgg
     2041 agcgcgtatc aggcggggca aagtccgttc gtgacgtttt tctctaaact gggtgtgcca
     2101 tatatcggca gcattatgaa cattgtggtg ctgaccgctg ccctctccag cctgaactca
     2161 ggtctgtact gcaccggacg tattctgcgc tcaatggcga tgggcggttc cgcaccgagt
     2221 tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc gacactagtt
     2281 gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt tgagattgtg
     2341 ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt gtgccagatg
     2401 cgcctgcgta aagcaattaa acaaggcaaa gcagcggatg tcagttttaa actgcctggc
     2461 gcgcccttca cttcctggct gacattactg tttttactga gtgtcctggt gctgatggcg
     2521 ttcgattacc cgaacgggac ttatactatc gcggcgctgc cgattatcgg tattctgctg
     2581 gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac tgcgccagtc
     2641 gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc gagctaattg
     2701 caaaattgcc cggctacgtg ccgggcaaat ttagattact gacgaaggtg tgccgcatgc
     2761 ggcgtgataa aagtcaggca agtctctccg ttttcaccct cgccacatct attacagcgc
     2821 aatacgaatc acatcgtccg gctgggtagc ttcctgctgt ttagtggatt tttgtttcac
     2881 actgacatac agcgttttgc catcggcaga cagcgccagg ctgtttggat gagtcggcgt
     2941 atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc acactgactt tacctgcctg
     3001 acgatgcgtt acgtaggctt cattacgcgc tgggttaaac agcacagcca gtgattctgg
     3061 tgccgcaacc ttcgccagaa tattgccatt acgggtatcg accactaaca cttctgcggc
     3121 tttagaatcg gtgataaatg cacgctgcct ggcggtatca aggctaatgt tgataaagaa
     3181 gtgctctttg ccgtcatcca gcagcttttt acggctgagg attttattgt cggcggtgtc
     3241 gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt tgctatccag
     3301 cgccagaccg gtactcattt taccggtgtt ctggatggcg gttttcagtt tgatattctc
     3361 gccatcaacg acccaaatca cgctctcttt accaatacca ctgatgtaaa cggtgttcgt
     3421 ggcatcatca gctaccagct cacgcggttg cagcgggcgc acctcttccg tgcgcttacg
     3481 atcatccagc accagacggc ctttcacctc gcccgttttg gcatctatcg ccgtgaccgc
     3541 gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg tggcaccaaa
     3601 cggcttgaga tcgttatgga tcgcctgcgt cacttccaga gtgaccggat caagacgata
     3661 aaccacgcca cctttatcca gtttgcggct ttgcgaagtg gcgagccaca gtgcgttttc
     3721 ttgctggcta taagccattt cgtaggcacc tttacctacc gctttacgca gcatttcttc
     3781 tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa cctaacagta atgaaccacg
     3841 caggcgcgat gaaaacagat gacgtaaatg catgacgact ccctttgatg aaccgatatt
     3901 tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt tataaatggg
     3961 aatggtaatc attattttct caaatgtgga gcatttcttg ccgttacgac tacaattttc
     4021 tcaattactt aacgtatcgt gccgtttcgt tttccataaa tttacaattt cattaccata
     4081 acggcgtttt cccattctta acctggcttt tttcatgaag attttttccg tccgacagac
     4141 cgttttgccc gcactgcttg tcctttcccc cgttgttttt gccgctgatg aacagactat
     4201 gattgtcagt gccgcaccgc aggtggtttc agaactggat accccagcag cagtaagcgt
     4261 ggtggatggc gaggagatgc gcctggcaac accgcgcatt aacttgtccg aatcactgac
     4321 cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt tacagctgtc
     4381 gattcgcgga tttggctccc gctccactta cggtattcgc ggtattcgcc tgtatgtgga
     4441 cggtattccc gccaccatgc ccgacgggca agggcaaaca tccaacatcg atttaagcag
     4501 tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca acgcgtctgg
     4561 tggggtaatg aatgtcacca cccagaccgg acaacagcca ccaaccattg aagccagtag
     4621 ttactacggc agttttggca gctggcgcta tgggctgaaa gcaacgggcg caacgggaga
     4681 cggcacacag cctggcgatg tcgattacac cgtctcaacc acgcgtttta cgacccacgg
     4741 ctatcgtgac catagtggcg cacagaaaaa tttagctaat gccaaactgg gcgtacgcat
     4801 tgatgaagcc agcaaattaa gtctgatttt caatagtgtg gatatcaaag cagatgaccc
     4861 aggtgggcta accaaagcag aatggaaggc taatccacaa caagcgcctc gtgcagaaca
     4921 gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg agcgtagcct
     4981 gagcgcgcaa gatgatatga gtgtgatgat gtatgccgga gagcgagaaa cgacccagta
     5041 ccagtcaatc ccgatggccc cccaacttaa cccgtcacat gcgggcggcg tgattaccct
     5101 gcaacgccat taccagggga tagacagccg ctggacacac cgtggtgaac tgggcgttcc
     5161 ggtcacgttc actaccggcc tgaactacga aaacatgagt gaaaaccgca agggctacaa
     5221 taacttccgc ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt tgcgtcgcga
     5281 cgaacgcaat ctgatgtgga acatcgatcc ctatttacag acgcagtggc agctgagcga
     5341 aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt ccaacgacca
     5401 ttacgttact ccgggtaacg gcgatgacag cggtgatgcc agttatcata aatggctacc
     5461 tgccggttcg ttaaaatatg caatgaccga tgcctggaat atctatctgg cagccgggcg
     5521 aggttttgaa acgccgacga ttaatgagct gtcttatcgt gctgatgggc aaagcggtat
     5581 gaactttggt ttaaaaccat ccaccaacga tacaattgag atcggcagta aaacgcgtat
     5641 tggtgatggg ctgcttagtc tcgcattgtt tcagaccgac actgatgatg aaattgttgt
     5701 cgatagcagt agcggtgggc gtacgactta caaaaatgcc ggaaagaccc gtcgtcaagg
     5761 cgctgaactg gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg cgtcctggac
     5821 ctggcttgat gcgacctatc gcagcaatgt ttgcaatgaa caggattgta acggtaatcg
     5881 gatgccaggg atcgcccgta atatgggctt tgcgtcgata ggttatgtac cggaagatgg
     5941 ttggtatgca ggcacggaag cgcgttatat gggcgatatt atggcagatg atgaaaatac
     6001 ggcaaaagcg ccgtcttata ctctcgtcgg cttattcacc gggtataaat acaattacca
     6061 caatttaact gtggatttat ttggtcgtgt cgataattta ttcgataaag aatacgttgg
     6121 ttctgtcatt gtcaatgagt caaacgggcg atattacgaa ccttcgcccg gacgaaatta
     6181 tggtgtcggc atgaatattg cgtggagatt tgagtaatta ccttgatgcc cggtatttgc
     6241 cgggcattta ctttaacgat tgtattgctg gtataaaata gcaatatttt gttgcataat
     6301 ttcagcaaga caatgtctgc atccctcttt gtcttttgct tttaacgcag cgagtaacag
     6361 atgatagttt tctatatgct cccgtaattc cgctggatta atcgcttcat agagataatg
     6421 taaaccaggc cccatcctga cccacagttg ctcaatcatc tcacacagga tgggcatatt
     6481 tgagcgatga taaatcgcta agcgaaatag cctgtttaca ttaatgattt gttccatgtc
     6541 gcccttttct tgcgcctgct gtaatttctc gagcaattcc tggagttccg ccaggtcttg
     6601 cggggtgagg ttttcaacag ccagagcaac tgccattaat tccagttcgt agcggatccg
     6661 attgatttcg tctaattgac gtttcctcac ttccggaact gtaaatgctt gtgcaggtgc
     6721 gacagaaagc gcattcaccg aaaccagacg taataatgct tcacgcacag gtgtaatact
     6781 catacctaat tgctccgcca gatttttagt aatcaggcgt gcgccaggtt ttaatgcgcc
     6841 aatactaagc tgatgtttca ggtcgttctc aacctgcaga gtcaaactga catgtttcat
     6901 ttttcccatt ccaggcattt taattccttc gcgtgttttc tgtccaaccc tgcctgcacg
     6961 gcagaaggag ttcacctggt gtattaaagt gaataatcat aaaccaccca cctttgttgt
     7021 tattagcagc agaacaatga cgtggttagt taattaaagc tgaaaataag ctttttgcct
     7081 ggatttttat ggcggcttac gccgccgttg aaactttaat catcacccgc gacgcggatc
     7141 accactttgc cgaagttttt acccttcagc aggccgataa acgtctgtgg cgcattctct
     7201 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
     7261 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
     7321 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
     7381 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
     7441 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
     7501 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
     7561 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
     7621 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
     7681 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
     7741 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
     7801 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
     7861 cccagcaccc agtcgccagg ctgataatca ggatgattcg actccacaac acggctcacc
     7921 gtgccgccga ccaacacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
     7981 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
     8041 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
     8101 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgcct cataatgctc
     8161 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     8221 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     8281 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     8341 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     8401 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     8461 ttgaagcgcg gaccatagca ggttatccgg tgctggtgag tgaagaagat ggcgtagtga
     8521 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     8581 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     8641 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     8701 aaaatcaggc ctcgctgcat ctgcaccagt cgctgggatt tgtcgtcacc gcacaaatgc
     8761 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     8821 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     8881 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtacgcatca cccagacctc
     8941 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     9001 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     9061 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     9121 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     9181 tctgatgctg gatatattcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     9241 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     9301 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     9361 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     9421 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     9481 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     9541 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     9601 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     9661 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     9721 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     9781 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     9841 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     9901 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     9961 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
    10021 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttatttg
    10081 atgtagtgat tttgcaagca gcacgtactg tcagagaaat caactctctg acagtacgca
    10141 agatcttctc atactctcca ggtttacaac attatcctta atatttactt ctttttgctg
    10201 gcatctacat tgaaatgagt agaaaacatc cccctactgt cgtctataca aacagcttac
    10261 tgattcaaac ggaatcttgt tttcacattt ttctatcatg aatactttat acgcgcggga
    10321 gtctataaat gctttatact ttcaaggaat aacgtaaata taaaaagatg gcgtaaagta
    10381 tatgcatggc aggatgtttg caacctcctt gatgacgctg ccccattgaa attgcaaacc
    10441 ccgccctggt tatcttgccc atttatcatg caaaacagat aattataggc ctctgaatac
    10501 ctcagaaa
//
LOCUS       DAZFDP010000156        10499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFDP010000156 DAZFDP010000000
VERSION     DAZFDP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..725)
                     /locus_tag="ACHJ1G_004132"
     CDS             complement(<1..725)
                     /locus_tag="ACHJ1G_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO4765891.1"
                     /translation="MRTSLVTREEMIEAIEQHTSCISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKANNVKLEKIHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTETLARGYGTETIRIRQNHHENKVRFVEGKHFFK
                     VEGESLRELKHRVALNYSVKIARNVRSLTLWTERGAARHAKMLETDQAWAFFEKLEDS
                     YFRQKEQQPVAIPQTLPEALRLAA"
     gene            complement(722..943)
                     /locus_tag="ACHJ1G_004133"
     CDS             complement(722..943)
                     /locus_tag="ACHJ1G_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4765892.1"
                     /translation="MATVPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(962..1081)
                     /locus_tag="ACHJ1G_004134"
     CDS             complement(962..1081)
                     /locus_tag="ACHJ1G_004134"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4765893.1"
                     /translation="MVVLAGLPKGRPVPFDAGSLNPVSATAIEIETSRGSSLT"
     gene            complement(1303..2787)
                     /locus_tag="ACHJ1G_004135"
     CDS             complement(1303..2787)
                     /locus_tag="ACHJ1G_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4765894.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNATIEEASKIPVGINSAGQWRVMTKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(2787..3980)
                     /locus_tag="ACHJ1G_004136"
     CDS             complement(2787..3980)
                     /locus_tag="ACHJ1G_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4765895.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGNHIAAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGASNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(4066..4488)
                     /locus_tag="ACHJ1G_004137"
     CDS             complement(4066..4488)
                     /locus_tag="ACHJ1G_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HFO4765896.1"
                     /translation="MEINHSRITSYEIADYMIRTKSLLSAKELAAILEKEYPHLDVDK
                     RDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRFDEKLQNELYM
                     TQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(4607..5650)
                     /locus_tag="ACHJ1G_004138"
     CDS             complement(4607..5650)
                     /locus_tag="ACHJ1G_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4765897.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQLFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            complement(5678..5857)
                     /locus_tag="ACHJ1G_004139"
     CDS             complement(5678..5857)
                     /locus_tag="ACHJ1G_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFO4765898.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(5862..6242)
                     /locus_tag="ACHJ1G_004140"
     CDS             complement(5862..6242)
                     /locus_tag="ACHJ1G_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO4765899.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(6242..6463)
                     /locus_tag="ACHJ1G_004141"
     CDS             complement(6242..6463)
                     /locus_tag="ACHJ1G_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO4765900.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(6536..6925)
                     /locus_tag="ACHJ1G_004142"
     CDS             complement(6536..6925)
                     /locus_tag="ACHJ1G_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4765901.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            complement(7047..7325)
                     /locus_tag="ACHJ1G_004143"
     CDS             complement(7047..7325)
                     /locus_tag="ACHJ1G_004143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765902.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            complement(7598..8278)
                     /locus_tag="ACHJ1G_004144"
     CDS             complement(7598..8278)
                     /locus_tag="ACHJ1G_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765903.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTRQLREKISRV
                     LGDVITPDHHKQAEKSGLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVADKH
                     EAGHANES"
     gene            complement(8260..8634)
                     /locus_tag="ACHJ1G_004145"
     CDS             complement(8260..8634)
                     /locus_tag="ACHJ1G_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765904.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPQTEVGELHLWLLGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRKKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            complement(8641..8934)
                     /locus_tag="ACHJ1G_004146"
     CDS             complement(8641..8934)
                     /locus_tag="ACHJ1G_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765905.1"
                     /translation="MWRGNSHGKSQMILTEYKLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            complement(9113..9346)
                     /locus_tag="ACHJ1G_004147"
     CDS             complement(9113..9346)
                     /locus_tag="ACHJ1G_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765906.1"
                     /translation="MGKLTFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKMELLTH"
     gene            complement(9429..10127)
                     /locus_tag="ACHJ1G_004148"
     CDS             complement(9429..10127)
                     /locus_tag="ACHJ1G_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4765907.1"
                     /translation="MHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAY
                     QGAREDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEAT
                     PENVEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQAGNFRENKGSSTNNFREISET
                     STNYPVTPDGWISCSERMPAQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGNAL
                     FWMLLPEPPQEAGQ"
     gene            complement(10328..>10499)
                     /locus_tag="ACHJ1G_004149"
     CDS             complement(10328..>10499)
                     /locus_tag="ACHJ1G_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765908.1"
                     /translation="EWVKKFSEQIFKITGDENAAKNELEPWTPEGVDPNYCWWDVDPV
                     DAANEAMSYHND"
BASE COUNT         2561 a         2762 c         2295 g         2881 t
ORIGIN      
        1 gcagccaggc gcagagcttc tggaagcgtc tgggggattg caaccggttg ctgttctttt
       61 tgccggaagt agctgtcttc cagtttttca aagaatgccc atgcctgatc ggtttcgagc
      121 attttagcgt ggcgggctgc gccgcgttct gtccagaggg tgagtgagcg aacattgcga
      181 gcaattttta cagagtagtt taaagctact ctgtgcttca actcgcgcaa tgattctcct
      241 tcaactttga aaaagtgctt cccttcaaca aagcgtactt tgttctcatg atgattttgg
      301 cgaatacgga ttgtttctgt cccataccct ctcgcaagag tctcggttgt cactacacgc
      361 actccctgcc attccagaac gggaatttca tcagactgat tctgaacaac caccagctcc
      421 gattcctgaa ctgaaggtgc atgaattttt tctaatttaa cgttatttgc tttcattctg
      481 tgtgcctcct tgcgtgcttc ggctgcgacg gttgcgtaat tcagatgacc ctgttcgagc
      541 aggtattcgc ggatatcaga caacaggata cggtgaaccg cgttcttgtc ctttctccgg
      601 taaagttgtt tggtgatcat gaagtagttg gcaataacgc ccggtatatc cctggtactg
      661 atacaggatg tgtgctgttc aattgcctca atcatttctt cacgggtgac taatgacgtt
      721 ctcatagtcc ctcctgagca gaagcgttaa cagggaggca ccagtaactg agagagttgc
      781 gcgaatcagt ggaaaagcgg gcagagaaaa tacagggagc atccggaagc tgagaacgtg
      841 cctcatcttc tgtcggtgca ataacgaagt gatagtgacg tttctggcag gaatagaagc
      901 gccagatgaa ttcaggatga gttggggtag ggacagtagc cataatggca gcctcctttg
      961 actaagttaa ggagctaccg cgtgaggtct caatctcaat ggcggtagca ctgactgggt
     1021 tgagactacc ggcgtcaaag ggaaccggcc tgcctttcgg cagcccagcc agcactacca
     1081 ttgatttcgg agctaaacgc tacgtatggc tgtgcgatgg catgacacaa aaaaagacgc
     1141 tttcggcgtc tgtgtcgcct ttgacattat ccggggtctc aatcccggca cccgttttaa
     1201 tgaggtgcct gataagcata aaccgaaaat gcctcaaggc gcaagaggtc aggttcaatg
     1261 taacatcggt agttaaaaaa cacaatttat tagagcaaat attcattcat taagccatgc
     1321 cagagcttca tcaacctgcg cttcgtcttc gacgctaagc acctcatcct ggggaacata
     1381 atccgccagc atagcgaaac aatatgtatc ccaatggtct ggtgagtgca ggttgagctt
     1441 tttcttcatg tcttccttag tcatcacccg ccattgacct gcggagttaa tacctacagg
     1501 tattttagac gcttcctcaa tggttgcatt acccttatcc agcctcatac gacctgattt
     1561 tacggcctct gcggcttgaa cgttggcata agcacgctta tcaaagtaca ggctcttatc
     1621 ttcacggcta tgcatctttt taccccagcg tatgcgctgt acggtaatac cataatactc
     1681 gtacatcaga tccgccgttg ctttacccag gccatcgccg tctatcgcta tggtgatatt
     1741 ggggaatcgc tcaggattac attctgcgaa aattttggcg gcaagctgcg tttctgtaac
     1801 gtctgtgtat tccagcattc gatagttgat tacacggcgt ttatttctct ggccggacac
     1861 catcatgata ttgataacgg acttatccct tcccgtacca ccagcaacgt ccacacatgc
     1921 aagccagccc catcctttgg caatcttgac tttccgccgc gttgcacgtt caacctcatc
     1981 acgtccaaga aggaagccat cctgtgattt agggaatagg ccgcgtacct taatcatgta
     2041 catagggtta tcgcgcccgc cgtactccgc cagcttcatt ttgataaatg ctggcgttac
     2101 cagcggtgat tcctcactgt taagcgtgat cgccgtataa acgccatcag ggttaccagg
     2161 acgcttggcc agtttatggt gagtatcgta gaaatagccg cttgggcgtg taggctgtga
     2221 cagtaataag atgcggttat cctgtccggt aagagcaccg gtgatgatac cgaaagctct
     2281 atcactgaca ccggaggctt catcgataat atacagaaga tgatctgcgt gttcaccggc
     2341 gagagcttct tcacttccca gacgaaagcc cttcggtact acagtccata cacctttacc
     2401 agtaacctca tagaaagcgg tttctgtcag aacaaaataa tcagcaagcc atgggaaacg
     2461 gctggtggca gtagcccagt ttatcttgat gtacttgaat ataccggtca ttacctgctg
     2521 aattttgttc gcaacgataa tggctcgggc accgggatac atgattatga acaacatgat
     2581 catgatagaa gtcatgtctg atttcccggt accgtgacca gacgaaacag atgtcttgct
     2641 accctgttcc tgcacagact caataatcag atcctgctgc caggtaggtg ttttgccgaa
     2701 caaaacatca gcggccgcaa tccagtcata acgatatagc gccaccagct cgcgccaacg
     2761 tggatccgtt acgcaacttc tggccattaa tcatcatccc cgtatagctt gcgggtaact
     2821 tcttcgtctt cctcctcgtc ttcgtccagg tcttgttcca gccatgggtc gtttgatgca
     2881 ccttcagtat caacatctcc ataaccgcct gtatcaacga tatcggcgat ttcttcccta
     2941 cgctgctcaa tccacaatgc ggcatccgcg cggcggttgg cggcccgttc tcgcgcaact
     3001 ttgtccagat cttcaagaga agggccaccg acggctgttt gcctttcctc atcatcggta
     3061 ttggtcttag gagcacgcag atcggctttg atttgctcca gcatcagggg cggcactttc
     3121 ccgccatgcg cctcgatgaa ttcagctgct tccagcactg accagttatt ttcacgcttt
     3181 cgttcgtatg ccagcttaac aatgccagct tgccccatag ataaagcgtg cttttccgcc
     3241 tcccggcttt cttttcgata gttattccgg atgctgtaaa tggtgttgat caggctgctt
     3301 atctgcgcgg aacagctgtt tagcatgctc gcgatacgat attcaggcgg agtcccttca
     3361 tcatcgtctt tttgctgatc gcgcatttcc tgcaccagac gaatacacgt atccctggcg
     3421 ttctccagca taaggagatg agaaagagac ttttccagaa gagtggtttc cagaacatcg
     3481 gccccggacc gacgcaacat agcgcgcgcg gccttccgcg cttcaacgtt atctatcagg
     3541 taatcgccag cttcgaattc aaagcgttca ccatcatcat ccagggtgtc gcgttccagg
     3601 cgatcacgta aggtccggtg ggcgcgggtg atcacgtcat gatcatcaga acgatcattt
     3661 atgcgcttat tttggcgctt cgcattctcg actgcggcac tgacaacggc attaaccctt
     3721 tgtttttccg ctatttcagc cgcaatgtga ttacctgcat gttgatcatt agagtgatca
     3781 atgatcatgc tttttagtgg cttcctgact ggcttatttg gcttgcggct gtccgctgtc
     3841 ctggtgtctt ctttgaaggc acggagataa cgacgtgcag tattagggtt aagattaaac
     3901 tcggcggcat actgtgcgat ggtgtaacca ccatctcgcg ccaggcgagc aaaattcttc
     3961 ttgtgatcgt cccaggtcac ttatgcttcc tttcgtaaaa actctttttg acgcgagggt
     4021 aacgaaagtc acatgtcaaa aggcccggaa cgggcaagca atcaatcaga tacgtgcgga
     4081 tgtggcatta ccgtaatgac ggtgctgacg ggccacctta ttgaaaagtt gacgcgccat
     4141 tacccaaggc tggtgctccc ggcgttcctt ttcgtcctgc gtcatataga gttcgttctg
     4201 gagtttttca tcaaaccggc gcggagcgcg gctacggcga aagaattcag gattcagaga
     4261 gtggatctga aatctacgtg ggcgtgtact gtcatcaatc aaaacagacg aatacttaga
     4321 cacagcgata gcctttaagc gcagataaac atcgcgctta tcgacatcca gatgcgggta
     4381 ttccttttca agaattgctg cgagttcttt cgctgataga agagatttag tgcggatcat
     4441 gtaatccgca atctcgtacg atgttattcg tgagtgattt atttccatga agtggcgtcc
     4501 ctgccagtta agtaacatcc tgtcacctac tgattagccc atgtcaacta atcaacgtgg
     4561 aatataatac cctcgattaa agaaatcgca atacattaga gcaattttat ctaacgctcg
     4621 acgagtgact tgtgatagcg ccgactccaa gcgcgtaatc aaagaacaat cgttgatgca
     4681 tcgccagcct accgtgcgtc ttctcccaat tatcgcggtc acgctcaata tcacgctggc
     4741 atgactggca cagaggaatt gcgtaaatgt catgcgcgca taatcgacta tgacgaacga
     4801 tataaggcgt aatgtgagcg ccagctcccg ccgctccaca cccacagcat ggacgggaag
     4861 caacaaagtc catgtactca ggtaatttta gcgattgcag ttttggtatt ttgaaatgcg
     4921 ccatgccagg gtcggagtca acatccacag ggcatacttt tgcacgcatc ggcgcggcgc
     4981 gttcttccat catctgaaca tatgctgtag cgcgatcgtc atacgggcga atatccgcct
     5041 ctttcagagg tccgctatcc tgcgttgcgg ctttcatctt atttattgat atacggcaaa
     5101 cttcttccgg catcaggtgc atcatgttgc gcatgaaagc ccaccagcac agttcctgaa
     5161 tacttaaatc atggccatct gaaagcccca tttcctgacg ggcgacatcc agtatccagt
     5221 taacgcgatt attatgcagc gtttctttca gctcattaaa accacgcatc cggtaatggt
     5281 tatcgtgatg ccagcacaac aacaccgcgc tattgtctcg ttctgcgtgg acaatatggt
     5341 tgtcacacca gctacgatct gcggcctggc attgcccctc tttcctgcgc aaccacgcca
     5401 ccagcgcgtc aattccacca atacggcgaa acagttcatc gctgttaaaa aacagctgca
     5461 acgcctcatt tgttgccata gtttgctcgg aaacaacgag gccgtcgtcc atgtgctcga
     5521 ttaactcacg cggcaccggc tccataataa atttacggcc agcctccacc agctttctga
     5581 cttcctgatc cactttgaat gtggcgacgc caagctcttt ttgtacaaag ggagtaatta
     5641 cggctttcac atcacacctt tcatcactga ttgggcttta tctgctgccc ggcattctct
     5701 gtttaagcac aaccatttcc tgacggcata acacagcaat agcggtcctg gcaccaattt
     5761 gcttaccaac caggtattgc tttacctcgc ggcgactcac gccatcaaga agcatcttta
     5821 acgcttcacg ggacaatttg ttgtatttac gtgccattaa tctactccgc ggaaccatac
     5881 aatctacgta acgtgtcggc gacagaagat acagatatct cgccagtcgc agcgcctaca
     5941 gtaaggtctg ccagttcagg tgaatcaaat acctgcaccc cgttacggcg tagaaatagc
     6001 agcgcgctgt ttagcgcggt acgcttattg gcatcattga atatatgccc tctcgctgta
     6061 gccaccaggt aggtggcgga gacttcgaaa aggtcggtga tctcttcgta ggcaactctg
     6121 gcctgaactc tcccgataat ggcctctgcc ctgcccggat ctgacatgcc aggcaggccg
     6181 ccgtagcggc ttatattcgc atcatgaagc gcaataagtt cttccggtga tatatgcctc
     6241 attatcggtt aaccagttcc ttgttggtgg agtccagggt gtcaaacagg gatgcaaatt
     6301 cagcatccag cgccgctttt ttgtaggctt cgaaagtagc cttgctgaca attactgctg
     6361 gctcacggcc tctgcgggtg atttcaacct cttccccggc ttcaacattg ttgagcactt
     6421 cagaaaggtt gccgcgcgcg gtacggaagt taatggattg cataaacacc tcgtgtactc
     6481 gttatgtgta cacaattata aacctaacag gcataaagca ccagcccttt gcagcttaaa
     6541 taaccggaca atcatcaaac tccccacttc gggcatcatt gatgacatga gtgatcacac
     6601 caaaaacagc attactgccc gtgtatccat cgtcatctac tggtaacgcc tctttcttcc
     6661 cggtgcttaa atcctccagg tgctggcgcg gatactttcg gtatctcttt atgcgatatt
     6721 caccctccat agcgcagaca agcagcgaac catcaaccgg agtaagcgag gaatcaacca
     6781 ccagcaaagc accctgcaat attccctcac ggtgatggct gtctgccgcc cgcatgaagt
     6841 aggtcgctga tggatgcctg attagttgct gatcaagaga aattctgctt tcaacataat
     6901 ccgccgcagg agaagggaag cccatagcgt tttcacctca acaatactgt tcatttatac
     6961 agtatacatt aaagagacac ctttggtgca aactcgttat gtacaccaac caccgcagat
     7021 gattttgtgc gctttgctac tattcatcac caatggatca gcgtaatctc attgccaatc
     7081 tgcagaattt tatccaccag cgatgtgatt ttttctgcca ggtcatcatt acttacccaa
     7141 tcctggcggg cagccagaac gggcgacaag gctaatatta actctcgtcg taatgatacg
     7201 ttccttgcta caggcggaat acgtggtgct ggtggtggtt taagcccctc gctcccttgt
     7261 atttctggag tattcggctg atagccatta gttattttcg gcggtattgg acgaggacga
     7321 aacatcgcgc tacctcctac actcagggca ggtgttccct tccgcaaagt aggggaaact
     7381 gctatcaagt tcatcacacc atctgcgatg ctcgtagcac caaaaagcct cccacggtaa
     7441 accaaaagac ttcatccacc gggacactcg atctggtata tctgctggtg gcagttcttc
     7501 tggcaataat ttgcctgata attccctttc cgcccgcgcc agcattcctt ttagactcgc
     7561 attctctttt tcaagaatct ctatgcgcgc ctgtaactca gctttcgttg gcatggcccg
     7621 cctcatgctt atcagccacc agcggcaata aagccctggc catcttatga accaatagtg
     7681 catcaataat gccaagcgta tgccccggct taatgtttaa tgccgcctca aggtgacacc
     7741 tttccaggcc acttttctcg gcttgtttat gatgatctgg cgtaataacg tcgcccaaaa
     7801 cacggctaat tttttctcgt aattgccggg tgcctgcaca cttgatcgct gtatcgtgga
     7861 gacggttaac cagttcgcga taaacatgcg gcttaatgcg gatacgttca ccggtgacgc
     7921 cctttcctgg cgctggcacc gaactatccg gaatatccgg atagttgcca gcctcgtaag
     7981 ctacccgcag ccagtgcatg aatgtttcag tagacacaca accacagtcc acatcgattt
     8041 ttccgcgttg ctgttccagc cactgctcaa aattcaatct acacgtatta ctttcatgtt
     8101 gctctttttg tcttaaggcc agcacctgtt gggccagttc cagaacgata ccgggtgacg
     8161 ctaacctctc aaattccaat aaatagtttg cgtcaggatg acaagtagcc tcgcctgcaa
     8221 aaaataccaa ttgctgtaag tatgctgtcg ttagagtagt catttctttt tgcgccattt
     8281 cttttcacat tccttagtcc atttttcaat gttcattttg gcaatatcag tcattccatc
     8341 acctaagaaa tactttttcc ggtacgtctt gcacttaaac cacactacaa cagccaccag
     8401 ccagaaaata aaaggccata cagcaatacc aactccagcc gcgataaagc ccaatagcca
     8461 taaatgaagc tctccaactt ctgtttgcgg caatattctt aaagaattaa acagcaggct
     8521 gaacgaatgg tcgtatgcat tggcagtata agacatgcaa tccatataat taaagtcata
     8581 gcctgcggct gccgcccata atgggcggtc aagaaaatgt tttagtgtca tcatataaat
     8641 ttaaggttca gaccagttat cttcaatagc aatgcttaat ctttgtagcc attctgctaa
     8701 tttcagcatt gcttctcttt cgcttaaacc acgaggaaaa tcatcaagcg atattgttgg
     8761 cttgaagccc ccgtaattat ccatttcaac ggtcagattt tgctccagta cggtattcct
     8821 tacgcggcta ttgtggcgaa gcaaatatac tgaacgtgat ttattggttt tgtggtctag
     8881 cttatattcg gtaagtatca tctggctttt gccatgacta ttacctctcc acatacttac
     8941 ctcacttaat aaaacaactc catgcgtagt tgatgatttt ttcccacgca atataaatct
     9001 gcactccggc agtaaaacca aagccaacaa ttgctgaaaa aatcaaaaca tttacttttg
     9061 acattataaa ttttctctcg gtgtcgtagg tgatagcacc ataattgata atttagtgag
     9121 ttagcagttc cattttttgg atgatttccg catgagcatc atcgttatca acacttaact
     9181 cgtttaatgc ctctcgcacc acatcaactt cttctggttg gaagaaatca tctcggtagt
     9241 caccaaatag aaccgaaaca agcctgccac cagcaacatc aagattggcg ctaacaggtg
     9301 gctctttacc atcctcaaat tcgactacaa aagttaattt ccccatcgtt accaccagcg
     9361 acaaattgaa tacaaaccca gtgctgccgc catcacaatt cctaccgttg cgaatgcttc
     9421 aggccagctc attgacctgc ctcctgggga ggttctggta gcagcatcca gaacaaggcg
     9481 ttccctaacc acgataaagt gccgtcgctc aactccacgt attccccttg cacctgtcct
     9541 gccatatact caccgtgctt tgaataaatt aaaatccaat catcttgagc gggcattcgc
     9601 tcactacagc ttatccaacc atccggagtt accggatagt tggttgacgt ttccgagatt
     9661 tcccgaaaat tattggttga cgaaccctta ttttcccgaa agtttccagc ctgaagcatg
     9721 gcggcgcggc aggcgttcca tatttcggca gcaatatcgc gctcgctatc ggttaatttg
     9781 tacgttgaaa catagccaga gagcatttct acgttttccg gagttgcttc ttcaggcact
     9841 accggtgctg gctcacgtat tacaggctcg cccatgcgtg attctccctg cgcctctttc
     9901 accatgtggt cattgatttg ctccagtcgc cgaacgtgct catcagcttc aagcgctcgc
     9961 cgtttccaga tggacaggtc ttcacgagcg ccctgatatg cgtcaccgta ttcgccgtta
    10021 aatactggcg ctggcggggc gatgcgtcca agcaacttat ttacctcttt cgccatcgcg
    10081 tcatatttat ctaaatagcg attagcttct aagcagactc ggtgcatctg atctgagtta
    10141 actcgtttaa ctggatctgc ttccagctat gccagtgcaa ttttgaataa ctcgccctat
    10201 acccgtgcca tccctgaatt ggggtagcat ttcgcaatcg ctatttttaa tttggcttct
    10261 tcgattaatt tctctttggt taattcagtc acttttcatt accgcccttt cgggcggcct
    10321 cctgacatta atcgttgtga taactcatag cttcatttgc agcatcaact ggatcaacat
    10381 cccaccagca ataatttggg tcgacacctt caggtgtcca cggctctaat tcattttttg
    10441 ccgcattctc gtcgccagta attttaaaaa tctgctctga gaactttttc acccactcg
//
LOCUS       DAZFDP010000157        10402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFDP010000157 DAZFDP010000000
VERSION     DAZFDP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..686
                     /gene="ssuE"
                     /locus_tag="ACHJ1G_004150"
     CDS             111..686
                     /gene="ssuE"
                     /locus_tag="ACHJ1G_004150"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4765909.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            679..1638
                     /gene="ssuA"
                     /locus_tag="ACHJ1G_004151"
     CDS             679..1638
                     /gene="ssuA"
                     /locus_tag="ACHJ1G_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4765910.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1635..2780
                     /gene="ssuD"
                     /locus_tag="ACHJ1G_004152"
     CDS             1635..2780
                     /gene="ssuD"
                     /locus_tag="ACHJ1G_004152"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4765911.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2791..3582
                     /gene="ssuC"
                     /locus_tag="ACHJ1G_004153"
     CDS             2791..3582
                     /gene="ssuC"
                     /locus_tag="ACHJ1G_004153"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO4765912.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3579..4346
                     /gene="ssuB"
                     /locus_tag="ACHJ1G_004154"
     CDS             3579..4346
                     /gene="ssuB"
                     /locus_tag="ACHJ1G_004154"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4765913.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4553..7165)
                     /gene="pepN"
                     /locus_tag="ACHJ1G_004155"
     CDS             complement(4553..7165)
                     /gene="pepN"
                     /locus_tag="ACHJ1G_004155"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4765914.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7431..8633
                     /gene="pncB"
                     /locus_tag="ACHJ1G_004156"
     CDS             7431..8633
                     /gene="pncB"
                     /locus_tag="ACHJ1G_004156"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4765915.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8802..10202
                     /gene="asnS"
                     /locus_tag="ACHJ1G_004157"
     CDS             8802..10202
                     /gene="asnS"
                     /locus_tag="ACHJ1G_004157"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4765916.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2353 a         2689 c         2796 g         2564 t
ORIGIN      
        1 agaaaaagat acacaaaatt tatatttggc attttcttgt ctctccgaca gactgaccga
       61 attgttatca atgttaacaa aaaaagaaca attggttata aggagagagt atgcgtgtca
      121 tcaccctggc gggtagtcct cgctttcctt ctcgctccag ctccttgctg gaatatgcgc
      181 gggaaaaact aaattgcctg gatgtagagg tttatcactg gaatctgcaa aacttcgccc
      241 cggaagatct gctttacgct cgtttcgata gtccggcact caagaccttc accgaacagc
      301 tgcaacaggc tgatggactg atagtcgcta cacctgtgta taaagccgcc tattccggcg
      361 cgctgaaaac cctgctcgac ctgctgccag agcgcgcttt gcaaggcaaa gtggtgctac
      421 cgctggcgac gggcggtacg gtggctcatc tgctggcggt cgattatgcc cttaagccag
      481 ttttaagtgc gatgaaagcc caggagatcc tgcacggcgt gtttgccgat gactcacaag
      541 ttattgatta ccatcacaaa ccccagttca cgccaaatct gcaaacccgt cttgataccg
      601 cgctagaaac tttctggcag gcattgcacc gccgcgatgt tcaggttcct gaccttctgt
      661 ctctgcgagg taatgtccat gcgtaacatc attaaactgg cgctggcggg attgcttagc
      721 gtctctactt ttgcggttgc tgcagaatcc tcgcctgaag cgttacgtat aggctatcag
      781 aaaggcagta ttggtatggt attggcaaaa agccaccagt tactggaaaa acgctatccg
      841 caatcaaaaa tctcctgggt ggagttcccc gcgggtccgc aaatgttgga agcgttaaac
      901 gttggcagta ttgatctcgg cagtaccggg gatattccgc caatctttgc ccaggctgcc
      961 ggggctgatt tggtatatgt gggcgtcgag ccaccgaagc ccaaagccga agtgattctg
     1021 gtggcagaaa acagcccgat caaaaccgta gccgatctta aaggtcacaa agttgccttt
     1081 cagaaaggtt ccagttcaca caacctttta ctgcgtgcac tgcgtcaggc cggacttaag
     1141 tttaccgata tccaacccac ttacctgacg cccgctgatg cccgcgccgc gttccagcaa
     1201 ggtaacgttg acgcctgggc tatctgggat ccctactact ctgctgcatt attacagggc
     1261 gccgtgcggg tgctgaaaga cggcaccgat ctcaatcaaa ctggatcgtt ttatctggca
     1321 gctcgtccct atgcagaaaa aaacggcgct tttattcagg gcgtactcgc aacctttagt
     1381 gaggccgatg cgttaacccg cagccagcgc gagcaaagca tcgctttact ggcaaaaacg
     1441 atgggcttac cggcaccggt gattgcctct tacttagatc atcgccctcc taccaccatc
     1501 aaaccggtta acgccaaggt tgccgcctta cagcagcaaa cggcagatct gttttatgaa
     1561 aatcgtctgg tgccgaaaaa agtcgatatt cgccagcgca tctggcagcc cactcaactg
     1621 gaaggaaaac aattatgagt ctgaatatgt tctggttttt accgacccat ggtgacgggc
     1681 attatcttgg aacggaagaa ggttcacgcc cggttgatca cggctatctg caacaaattg
     1741 cgcaagcggc ggatcgtctg ggctataccg gtgtgctgat cccaacgggg cgatcgtgtg
     1801 aagatgcgtg gctggtggcg gcgtcgatga tcccggtgac gcagcggctg aagtttcttg
     1861 tcgccctgcg ccccagcgta acctcaccta ccgttgccgc tcgccaggcc gccacgcttg
     1921 accgtctctc aaatggtcgt gcgttgttta atctggtcac aggcagcgat ccacaagagc
     1981 tggcaggcga cggcgtgttc cttgatcata gcgagcgcta cgaagcctcg gcggaattta
     2041 cccaggtctg gcggcgctta ttgctggggg aaaccgtcaa tttcaacggt aaacatattc
     2101 atgtgcgcgg agcaaaactg ctcttcccgc cgattcaaca gccttatcct ccgctttact
     2161 ttggcggatc gtcagacgtt gcccaggagc tggcggcaga acaagttgat ctctacctca
     2221 cctggggcga accgccagaa ctagtaaaag agaagatcga acaagtgcgg gcgaaagctg
     2281 ccgcgcatgg acgcaaaatt cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta
     2341 acgacgaagc gtggcaggcc gccgagcggt taatctcgca tcttgatgat gaaactatcg
     2401 ccaaagcaca ggccgcattc gcccggacgg attccgtagg acaacagcga atggcggcgt
     2461 tacataacgg caagcgcgac aatctggaga tcagccccaa tttatgggcg ggcgttggct
     2521 tagtgcgcgg cggtgccgga acggcgctgg tgggcgatgg tcctacggtc gctgcgcgaa
     2581 tcaacgaata cgccgcgctt ggcatcgaca gctttgtgct ttcgggctat ccgcatctgg
     2641 aagaagcgta tcgggtcggc gagttgctgt tcccacatct ggatgtcgcc attccggaaa
     2701 ttccccagcc gcagccgctg aatccgcaag gcgaagcggt ggcgaatgat tttatccccc
     2761 gtaaagtcgc gcaaagctaa ggaagaaata atggcaacgc cagtgaagaa gtggttattg
     2821 cgcgttgccc cctggttttt accggtgggc atcgtggcgg tgtggcaact ggcctcctca
     2881 gttggctggc tgtcgacgcg tattttgccc tcaccggaag gggtggtgac ggcgttctgg
     2941 acgctctccg ccagcggcga actgtggcag catctggcga tcagttcctg gcgggcgctg
     3001 attggctttt caattggcgg atcgctgggg ttgattttgg gactgattag cgggctatct
     3061 cgctggggag aacggctgct ggatacctca attcagatgt tgcgcaacgt accgcatctg
     3121 gcgctgatcc cgctggtgat tttgtggttt ggcattgatg aatccgcaaa aatctttctg
     3181 gtggcgctcg gcacgctatt ccccatttat atcaacacct ggcatggcat ccgtaatatc
     3241 gatcgcggtc tggtggaaat ggcgcgtagc tatggattat ccggcatacc gctgtttatc
     3301 catgtgatcc tgcctggtgc cctgccctca attatggtcg gcgtgcgttt tgcgttgggc
     3361 ctgatgtggc tgacgctgat tgttgccgaa accatttctg ccaattcagg cattggttat
     3421 ctggcgatga atgcgcggga gtttttgcaa acggacgtgg tggtagtcgc tattattctt
     3481 tacgccctgc tcggcaaact tgctgacgtc agcgcgcagc tgttagagcg cctctggttg
     3541 cgctggaacc cggcttatca tttgaaggag gccacggtat gaatactgct cgtctgaacc
     3601 agggcacacc attgttgctc aatgcagtaa gcaaacatta cgcggaaaat atcgtcctga
     3661 accaactgga tttacatatt ccggcaggtc agtttgtggc ggtggtgggc cgcagcggtg
     3721 gtggcaaaag taccctgctg cgcctgctgg caggtctgga aacgccaacc gcaggcgatg
     3781 tgttagcggg caccacaccg ctggctgaga ttcaagaaga cacgcgaatg atgtttcagg
     3841 atgcgcgtct gctgccgtgg aaatcggtga ttgataacgt tgggttaggc cttaaaggcc
     3901 agtggcgcga tgccgcgcgt cgggcgttgg ctgcagtagg actggagaat cgcgccgggg
     3961 aatggcctgc cgcactttct ggcgggcaga aacagcgtgt ggcgctggca agagcgttga
     4021 ttcatcgacc gggtttattg ttgcttgatg aaccgctcgg ggcgctggac gccttaacgc
     4081 gactcgagat gcaggatttg attgtgtcac tttggcagca gcacggcttt accgtactgt
     4141 tggtgacgca tgatgtgagc gaagcggtag caatggctga ccgggtgttg ttaattgaag
     4201 agggaaaaat tggtctggat ttgacagtgg atatcccccg tccgcgtcga ttagggtcgg
     4261 tgcgattagc ggagctggaa gcggaagtat tgcaacgggt aatgcggcgt gggcattctg
     4321 aacaactgat tcggcgtcat ggttaatatg ccggatgtgg cgcacaaaac cagcattttc
     4381 aacggactag taggcctgat aagacacgtc aagcatcgca tcaggcagtt gggcacggct
     4441 gccggatgcg gcgtgaacgc cttatccagc ctacaaaacc agcaatttca acggagttgt
     4501 aggcctgata agacgcgtca agcgtcgcat caggcatttt ggctcaaaag tatcaagcca
     4561 gtgctttggt gatcttctcg tacagatcgc cagagagatt ttccagccct ttcaactgtt
     4621 ccagcgccgc gcgcattttc tcctgacgtt tggcatcata acgtttcagg cgaatcagcg
     4681 gttcaatcag acgtgaagct acctgcgggt tacggctgtt gaggtcggta agcatttcca
     4741 ccaggaactg gtaaccgctg ccatcttcgg catggaacgc tgccgggttg ctgcccgcaa
     4801 acgcaccaat caacgaacga atacggttcg ggttgctcat ggtaaatgag cgatgctgca
     4861 acagaccacg caccgtctcc agcacattcg ccgccgggct ggtggcttgc aggataaacc
     4921 atttatccat caccagaccg tcctgatgcc acttatcgtc gtactcctgc atcagcgcgt
     4981 cacggcaagg cagctgtgca gcaaccgccg cagaaagcgc tgccagcgca tcggtcatat
     5041 tgtttgcttc atggtattgc ttgctcacca gcacgtcagc cagatgcgtt tcaccaaaag
     5101 caaggaagcg caggcaggca ttacgcagag tgcgttttgc aatatcttca tgctcaacac
     5161 ggtactcgct ctggtaattc gcgttgtaaa tagccagcag ctcatccgcc agttcagtcg
     5221 ccagagtacg agtgagtgct tcgcgtactt cggcaatagc aatcgggtcg atgatatcga
     5281 acagttcagc catttcattg acagaaggca gcgtcaggat ttctgccgcc agcgccggat
     5341 caatcttctc atcgagcagc accgcgcgga aagcgtcagc cacatgtacc ggcagagaca
     5401 gcggctgccc ttgctgatga cgggcgacgt tcagcttgat gtaggttgcc agcaaacttt
     5461 gcgccgcatc ccaacgggag aaatcattac gcgcatgacg catcaggaag gtcagttgct
     5521 gatcgctcca cttatattcc agtttcactg gcgcagagaa ttcgcacagc agcgcaggca
     5581 ccggctggaa gtagacatta tcaaaaacaa aggtctgttc cgcctgggtg acgttcagca
     5641 cggaattcac cggatgaccg cctttctgca acgggatcac tttgccttcg ttgtcataca
     5701 attcgatggc aaatggaata tgcagcggct gtttttctgc ctgatccggc gtggctggcg
     5761 tgcgctggct gatggtcagg gtgtactgct cggtttccgg attgtagtcg tctttgacgg
     5821 tcacaatcgg cgtgccggac tggctgtacc aacggcggaa atgggagaga tcgacattcg
     5881 acgcatcttc catcgcctgc acaaagtcgt cgcaggttgc cgcgctgcca tcatggcgct
     5941 cgaaatagag ttgcatccct ttctggaagt tttcttcgcc cagcagggtg tggatcatgc
     6001 gaatcacttc cgcgcccttc tcgtaaacgg tcagggtgta gaagttgttc atctcaatga
     6061 ccatatccgg gcggatcggg tgcgccatcg ggctggcgtc ttccgcaaac tgcaatccgc
     6121 gcatggtgcg cacattattg atacggttta ccgcgcggga accaaggtca gagctgaatt
     6181 cctgatcgcg gaagacggtt aaaccttctt tcaggctgag ctggaaccag tcgcggcagg
     6241 tcactcggtt accggtccag ttatggaaat attcatggcc gataacgcgt tcaatatcga
     6301 gataatcttt gtcggtggcg gtgtcggtgc gggccaacac atatttggag ttaaagatat
     6361 tcagaccctt attctccatt gcgcccatat tgaagaaatc caccgcgacg atcatataga
     6421 tgtcgaggtc atactccagg ccaaagcgtt cttcatccca tttcatggag tttttcagcg
     6481 aggtcatcgc ccacggcgcg cgatcaaggt tgccgcgatc gacgtacagc tccagtgcta
     6541 cttcgcgacc agaacgcgtg gtaaaggtgt cgcgcagtac atcaaagtcg cctgccacca
     6601 gcgcaaacag gtagcacggt ttcgggaacg ggtcctgcca ctgtacccaa tggcgtccgt
     6661 tttccagttc accttgcgca acgcggttac cgttggaaag caggaaggga tatttggttt
     6721 tatcggcaat aattttggtg gtaaaacgcg ccagcacgtc cgggcggtcg agataatacg
     6781 taatatggcg gaaaccttcg gcttcacact gggtgcaaag cgcatcgcct gactgataaa
     6841 gcccttccag cgccgtattc gccgccgggc ttatttcatt aatgatctta agcgtaaaac
     6901 gctccggcaa attactgatg accagtgcgc cctcttcttc tttccaggcg gtccacggct
     6961 catcattaat atgaacagaa accagtttga ggtcttcgcc gttgagacga agcggagcat
     7021 ctgatgcacc atgacggaca gcctggctga ccgcggtaac gaccgtcttt tgcgcgtcga
     7081 ggtcaaaggt caagtcaata tcagtaatct ggtaatccgg cgcacgataa tcgtgacggt
     7141 atttggcttg tggctgttga gtcataaata acctttagca tcttttatag agtctggtgt
     7201 tcagtctatt cctgttgcgt aaatcgcgct atgcagaatc ttcatctttt caggtacaaa
     7261 cgcctttatt gctacatttt tataacatac accgcgtaat gccatcgacc agaaaggtgg
     7321 catatggtgt gatcggggtt caataaattg cgaaacaagg tatactccag cagttcctga
     7381 agatgtttat tgtactaaac gctcctgtac gaggacgcta ctgcgcacct atgacacaat
     7441 tcgcttctcc tgttctgcac tcgttgctgg atacagatgc ttataagttg catatgcagc
     7501 aagccgtgtt tcatcactat tacgatgtgc atgtcgcggc ggagtttcgt tgccgaggtg
     7561 acgatctgct gggtatttat gccgatgcta ttcgtgaaca gattcaggcg atgcagcacc
     7621 tgcgcctgca ggatgatgaa tatcagtggc tctctgccct gcctttcttt aaggccgact
     7681 atcttaactg gttacgcgag ttccgcttta acccggaaca agtcaccgta tccaacgata
     7741 atggcaagct ggatattcgt ttaagcggcc cgtggcgtga agtcatcctc tgggaagttc
     7801 ctttgctggc ggttatcagt gaaatggtac atcgctatcg ctcaccgcag gccgacgttg
     7861 cgcaagccct cgacacgctg gaaagcaaat tagtcgactt ctcggcgtta accgccggcc
     7921 ttgatatgtc gcgcttccat cttatggatt ttggcacccg tcgccgtttt tctcgcgaag
     7981 tacaagaaac catcgttaag cgtctgcaac aggaatcctg gttcgtgggc accagcaact
     8041 acgatctggc gcgtcggctt tccctcacgc cgatgggaac acaggcacac gaatggttcc
     8101 aggcacatca gcaaatcagc ccggatctag ccaacagcca gcgagctgca cttgctgcct
     8161 ggctggaaga atatcccgac caacttggca ttgcattaac cgactgcatc actatggatg
     8221 ctttcctgcg tgatttcggt gtcgagttcg ccagtcgcta tcagggcctg cgtcatgact
     8281 ctggcgaccc ggttgaatgg ggtgaaaaag ccattgcaca ttatgaaaag ctgggaattg
     8341 atccacagag taaaactctg gttttctctg acaatctgga tttacgcaaa gcggttgagc
     8401 tataccgcca cttctcttcc cgcgtgcaat taagttttgg tattgggact cgcctgacct
     8461 gcgatatccc ccaggtaaaa cccctgaata ttgtgattaa gttggttgag tgtaacggta
     8521 aaccggtggc gaaactttct gacagccctg gcaaaactat ctgccacgat aaagcgtttg
     8581 ttcgggcgct gcgcaaagcg ttcgaccttc cgcatattaa aaaagccagt taatatcatc
     8641 agggagctaa tcggctccct ttttttacct ttaattccga aatctttcgc tgcatttgcg
     8701 aattctgctt gtctgattgc agatgccagg taacataggt atccccccat ttacgggtgg
     8761 acaagtgttt attttttccg actattaaca gagagaatat tatgagcgtt gtgcctgtag
     8821 ccgacgtact ccagggccgc gtagccgttg acagcgaagt caccgtacgc ggatgggtac
     8881 gtacccgccg agattcaaaa gctggcatct ccttcctcgc cgtttatgac ggttcctgct
     8941 ttgatcctgt acaggctgtc atcaataatt ctctgcccaa ttacaatgaa gacgtcctgc
     9001 gtctgaccac cggctgctcg gtcattgtga cgggtaaagt cgtggcgtcg ccgggccagg
     9061 ggcaacaatt tgaaattcag gccagcaagg ttgaagttgc tggttgggtt gaagatccag
     9121 acacttaccc gatggcggca aaacgccaca gcattgagta tctgcgtgaa gtcgctcacc
     9181 tgcgtccgcg cacaaacctg attggtgccg tcgcgcgcgt tcgtcatacg ctggcgcagg
     9241 cgctgcatcg cttctttaac gagcagggat tcttctgggt ttcaacgcca ctgattaccg
     9301 catctgatac cgaaggtgca ggcgaaatgt tccgcgtttc tacgctggat ctggaaaacc
     9361 tgccgcgtaa cgatcagggc aaagtggatt tcgacaaaga cttctttggt aaagagtctt
     9421 tcctgaccgt atctggccag ttgaacggcg aaacctacgc ttgcgcatta tccaaaattt
     9481 ataccttcgg cccgactttc cgtgctgaaa actccaacac cagccgtcac ctggcggaat
     9541 tctggatgct ggagccggaa gtggcgtttg ctaacctgaa cgatatcgcg ggtctggctg
     9601 aagccatgct gaaatatgtc ttcaaagcgg ttctcgaaga acgcgcagac gacatgaaat
     9661 tcttcgctga acgcgtagat aaagatgccg tttcacgtct ggaacgcttc attgaggccg
     9721 atttcgcgca ggtggattac accgacgcag tgaccattct cgaaaactgc ggcaggaagt
     9781 ttgaaaaccc ggtttactgg ggcgtcgatc tctcttctga gcatgagcgt tatctggcgg
     9841 aagaacactt taaagcaccg gtagtggtta aaaactatcc gaaagatatt aaagcgttct
     9901 atatgcgcct caacgaagac ggtaaaaccg ttgcggctat ggacgttctg gctccgggca
     9961 tcggtgagat cattggtggc tcccagcgtg aagaacgtct ggacgttctg gacgagcgta
    10021 tgctggaaat gggcctgaat aaagaagatt actggtggta tcgcgatctg cgtcgctacg
    10081 gtactgttcc gcattcaggt ttcggtcttg gttttgaacg cctgattgct tacgtaactg
    10141 gcgtgcaaaa cgtgcgtgat gtgattccgt tcccacgtac tccgcgcaac gccagcttct
    10201 gattttcttc ctcaaagagc cagcgcctgc tggctctttt ttatcctttc tgtcaagtta
    10261 tctgtttgtt aagtcaagca atctatttgc aaccccgcca taacgatcat cctgttacgg
    10321 aatattacat tgcaacattt acgtacaaaa aataacccgc attcttattg cggattagtt
    10381 ttttcttagc tgatagcaca at
//
LOCUS       DAZFDP010000158        10288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFDP010000158 DAZFDP010000000
VERSION     DAZFDP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..1528
                     /gene="galP"
                     /locus_tag="ACHJ1G_004158"
     CDS             134..1528
                     /gene="galP"
                     /locus_tag="ACHJ1G_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4765917.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1605..2102
                     /locus_tag="ACHJ1G_004159"
     CDS             1605..2102
                     /locus_tag="ACHJ1G_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4765918.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2197..2904
                     /gene="endA"
                     /locus_tag="ACHJ1G_004160"
     CDS             2197..2904
                     /gene="endA"
                     /locus_tag="ACHJ1G_004160"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4765919.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2984..3715
                     /gene="rsmE"
                     /locus_tag="ACHJ1G_004161"
     CDS             2984..3715
                     /gene="rsmE"
                     /locus_tag="ACHJ1G_004161"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4765920.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3728..4678
                     /gene="gshB"
                     /locus_tag="ACHJ1G_004162"
     CDS             3728..4678
                     /gene="gshB"
                     /locus_tag="ACHJ1G_004162"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4765921.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4787..5350
                     /locus_tag="ACHJ1G_004163"
     CDS             4787..5350
                     /locus_tag="ACHJ1G_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4765922.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5350..5766
                     /gene="ruvX"
                     /locus_tag="ACHJ1G_004164"
     CDS             5350..5766
                     /gene="ruvX"
                     /locus_tag="ACHJ1G_004164"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4765923.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5942..6922)
                     /locus_tag="ACHJ1G_004165"
     CDS             complement(5942..6922)
                     /locus_tag="ACHJ1G_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4765924.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6940..7644
                     /gene="yggS"
                     /locus_tag="ACHJ1G_004166"
     CDS             6940..7644
                     /gene="yggS"
                     /locus_tag="ACHJ1G_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4765925.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7662..8228
                     /gene="yggT"
                     /locus_tag="ACHJ1G_004167"
     CDS             7662..8228
                     /gene="yggT"
                     /locus_tag="ACHJ1G_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4765926.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8225..8515
                     /gene="yggU"
                     /locus_tag="ACHJ1G_004168"
     CDS             8225..8515
                     /gene="yggU"
                     /locus_tag="ACHJ1G_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4765927.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8523..9116
                     /locus_tag="ACHJ1G_004169"
     CDS             8523..9116
                     /locus_tag="ACHJ1G_004169"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4765928.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9109..10245
                     /gene="hemW"
                     /locus_tag="ACHJ1G_004170"
     CDS             9109..10245
                     /gene="hemW"
                     /locus_tag="ACHJ1G_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4765929.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2411 a         2726 c         2720 g         2431 t
ORIGIN      
        1 ttcatgcact taaaccataa ctaagataaa tgttagtgta agcgattaca ctgatgtgat
       61 ttgcttcaca tctttttacg tcgtactcac ctatcttaat tcacaataaa aaataaccat
      121 attggagggc atcatgcctg acgctaaaaa acaggggcgg tcaaacaagg caatgacttt
      181 tttcgtctgc ttccttgccg ctctggcggg attactcttt ggcctggata tcggtgtaat
      241 tgctggcgca ctgccgttta ttgcagatga attccagatt acttcgcaca cgcaagaatg
      301 ggtcgtaagc tccatgatgt tcggtgcggc agtcggtgcg gtgggcagcg gctggctctc
      361 ctttaaactc gggcgcaaaa agagcctgat gatcggcgca attttgtttg ttgccggttc
      421 gctgttctct gcggctgcgc caaacgttga agtactgatt ctttcccgcg ttctgctggg
      481 gctggcggtg ggtgtggcct cttataccgc accactgtac ctgtcagaaa ttgcgccgga
      541 aaaaattcgc ggcagtatga tctcgatgta tcagttgatg atcactatcg ggatcctcgg
      601 tgcttatctt tctgataccg ccttcagcta caccggtgca tggcgctgga tgctgggtgt
      661 gattatcatc ccggcaattt tgctgctgat tggtgtcttc ttcctgccag acagcccacg
      721 ttggtttgcc gccaagcgcc gttttgttga tgccgaacgc gtgctgctac gcctgcgtga
      781 caccagcgcg gaagcgaaac gcgaactgga tgaaatccgt gaaagtttgc aggttaaaca
      841 gagtggctgg gcgctgttta aagagaacag caacttccgc cgcgcggtgt tccttggcgt
      901 actgttgcag gtaatgcagc aattcaccgg gatgaacgtc atcatgtatt acgcgccgaa
      961 aatcttcgaa ctggcgggtt ataccaacac taccgagcaa atgtggggga ccgtgattgt
     1021 cggcctgacc aacgtacttg ccacctttat cgcaatcggc cttgttgacc gctggggacg
     1081 taaaccaacg ctaacgctgg gcttcctggt gatggctgct ggcatgggcg tactcggtac
     1141 aatgatgcat atcggtattc actctccgtc agcgcagtat ttcgccatcg ccatgctgct
     1201 gatgtttatt gtcggttttg ccatgagtgc cggtccgctg atttgggtac tgtgctccga
     1261 aattcagccg ctgaaaggcc gcgattttgg catcacctgc tccaccgcca ccaactggat
     1321 tgccaacatg atcgttggcg caacgttcct gaccatgctc aacacgctgg gtaacgccaa
     1381 caccttctgg gtgtatgcgg ctctgaacgt actgtttatc ctgctgacat tgtggctggt
     1441 accggaaacc aaacacgttt cgctggaaca tattgaacgt aatctgatga aaggtcgtaa
     1501 actgcgcgaa atcggcgctc acgattaatc tcctcaggct tcctcccatc gcggaggaag
     1561 ccacctcttg cagtcatctt ttcttcgctc tatcctctgc cgctatgaaa acatcccgtc
     1621 tccctatcgc catccagcag gccgttatgc gccgcctgcg ggaaaaactc gcccaggcca
     1681 acctgaagct agggcgtaac tacccggagc caaaactctc ttacacccag cgcggaacct
     1741 ccgccggaac agcctggctg gaaagctatg aaatccgcct caatcccgtt ttgctgttgg
     1801 aaaacagtga agcttttatt gaagaagtgg taccgcacga actggcacat ttgctggtat
     1861 ggaaacattt cggccgcgta gcgccacatg gcaaagagtg gaagtggatg atggaaagcg
     1921 tgctgggcgt tcccgcccgt cgtacgcatc agttcgaact gcaatccgtg cgtcgcaaca
     1981 ccttccccta ccgctgcaag tgccaggagc atcagcttac cgtacgccgc cataatcgcg
     2041 tagttcgtgg cgaggccgtc tatcgctgtg ttcactgcgg tgaacagctg gttgcgaaat
     2101 aaccatctga actatcagga actttcctga tctggctgat tgcataccaa aacaactttc
     2161 gctacgttgc tggctcgttt taacacggag taagtgatgt accgttattt gtctattgct
     2221 gcggtggtac tgagcgcagc attttccggc ccggcgttgg ccgaaggtat caatagtttt
     2281 tctcaggcga aagccgcggc ggtaaaagtc cacgctgacg cgcccggtac gttttattgc
     2341 ggatgtaaaa ttaactggca gggcaaaaaa ggcgttgttg atctgcaatc gtgcggctat
     2401 caggtgcgca aaaatgaaaa ccgagccagc cgcgtagagt gggaacacgt cgttcccgcc
     2461 tggcagttcg gtcaccagcg ccagtgctgg caggacggtg gacgtaaaaa ctgcgctaaa
     2521 gatccggtct atcgcaagat ggaaagcgat atgcataacc tgcaaccgtc agtcggcgag
     2581 gtgaatggcg atcgcggcaa ctttatgtac agccagtgga atggcggcga aggccagtac
     2641 ggtcaatgcg ccatgaaggt cgatttcaaa gaaaaagctg ccgaaccacc agcgcgtgca
     2701 cgcggtgcca ttgcacgcac ctacttctat atgcgcgacc aatacaacct gacactctct
     2761 cgccagcaaa cgcagctgtt caacgcatgg aacaagatgt atccggttac cgactgggag
     2821 tgcgagcgcg atgaacgcat cgcgaaggtg caaggcaatc ataatccgta tgtgcaacgc
     2881 gcttgccagg cgcgaaagag ctaacctaca ctagcgggac tctttttgtt aacccctacc
     2941 ccacgcgtac aaccgcgtgg ggagacgacg cggattttta actatgcgta tcccccgcat
     3001 ttatcatcct gaaccactga ccagccattc tcacatcgcg ctttgcgaag atgccgccaa
     3061 ccatatcggg cgcgtactgc gcatggggcc ggggcaggcg ttgcaattgt ttgacggtag
     3121 caaccaggtc tttgacgccg aaattaccag cgccagcaaa aaaagcgtgg aagtgaaggt
     3181 gctggaaggc aagatcgacg atcgcgaatc tccgctgcat attcacctcg gtcaggtgat
     3241 gtcgcgtggt gaaaaaatgg aatttacgat ccagaaatcg atcgaactcg gtgtaagcct
     3301 cattacgcca cttttttctg agcgctgcgg cgttaaactg gatagtgaac gtctgaacaa
     3361 gaagctccag cagtggcaga agattgcaat tgctgcctgt gagcagtgtg gtcgtaaccg
     3421 ggtgccggaa atccgcccag cgatggatct ggaagcctgg tgtgcagagc aggatgaagg
     3481 actgaaactg aatcttcacc cgcgcgccag taacagcatc aatacgttgc cgttaccggt
     3541 tgaacgcgtc cgcctgctga ttggcccgga aggcggttta tcggcagatg aaattgccat
     3601 gactgcccgc tatcaattta ctgatatcct gttgggacct cgcgttttgc gtacagagac
     3661 aactgcgctc accgccatta ccgcgctaca agtacgattt ggcgatttgg gctaacggag
     3721 aagaataatg atcaagctcg gcatcgtgat ggaccccatc gcaaacatca acatcaagaa
     3781 agattccagt ttcgctatgt tgctggaagc acagcgtcgt ggttacgaac ttcactatat
     3841 ggagatgggc gatctgtatc tgatcaatgg cgaagcccgc gcccataccc gcacgctgaa
     3901 cgtgaagcag aactacgaag agtggttttc gttcgtcggt gaacaggatc tgccgctggc
     3961 cgatctcgat gtgatcctga tgcgtaaaga cccgccgttt gataccgagt ttatctacgc
     4021 gacctatatt ctggaacgtg ccgaagagaa agggacgctg atcgttaaca agccgcagag
     4081 cctgcgcgac tgtaacgaga aactgtttac cgcctggttc tctgacttaa cgccagaaac
     4141 gctggttacg cgcaataaag cgcagctaaa agcgttctgg gagaaacaca gcgacatcat
     4201 tcttaagccg ctggacggta tgggcggcgc gtcgattttc cgcgtgaaag aaggcgatcc
     4261 aaacctcggc gtgattgccg aaaccctgac tgagcatggc actcgctact gcatggcgca
     4321 aaattacctg ccagccatta aagatggcga caaacgcgtg ctggtggtgg atggcgagcc
     4381 ggtaccgtac tgcctggcgc gtattccgca ggggggcgaa acccgtggca atctggctgc
     4441 cggtggtcgc ggtgaacctc gtccgctgac ggaaagtgac tggaaaatcg cccgtcagat
     4501 cgggccgacg ttgaaagaaa aagggctgat ttttgttggt ctggatatca tcggcgaccg
     4561 tctgactgaa attaacgtca ccagcccaac ctgtattcgt gagattgaag cagagtttcc
     4621 ggtgtcgatc accggaatgt taatggatgc catcgaagca cgtttacagc agcagtaacc
     4681 caccttagcg agaaggatct cgttgagact ctgagtgaca gcgcccttct ttccacgcat
     4741 actgggcgct gttgcttttt tgaaccagga aacagaacct ctgacaatga atttacagca
     4801 tcactttctt attgccatgc ctgctctcca ggatccgatt ttccgtcgtt ccgtggtcta
     4861 catttgcgaa cataatacca atggtgcaat ggggatcatc gtcaacaagc cgctggaaaa
     4921 tctcaaaatt gaagggattc tggaaaagct gaagatcacg ccggagccgc gtgatgaatc
     4981 aatccgtctg gataaaccgg ttatgctcgg cggtccgctg gctgaagatc gcgggtttat
     5041 tttgcatact ccgccctcca attttgcttc cagcattcgc atttcagaca acacagtgat
     5101 gaccacttcc cgcgatgtgc tggaaacgct cggcaccgat aaacaaccgt ctgacgtatt
     5161 ggtggctctg ggttatgcct cctgggaaaa aggtcaactg gaacaagaaa ttctcgataa
     5221 cgcgtggcta acggccccgg cggatctgaa tattctgttc aaaacgccga ttgccgaccg
     5281 ctggcgcgag gcggcaaaac tgattggtgt ggatattctc accatgcctg gtgtggcagg
     5341 gcacgcctga tgagtggaac cttactcgcc ttcgacttcg gcaccaaaag cattggcgta
     5401 gcggtcggcc aacgcattac cggcactgct cgccctttgc ctgcaattaa agcacaggac
     5461 ggtacgccgg actggaacct catcgaacgt ttactgaaag agtggcagcc ggacgaaatc
     5521 atcgtcggtt tgccgctgaa tatggacggc accgagcaac cattgactgc cagagcgcgt
     5581 aaatttgcca accgtattca tggtcgtttc ggtgttgaag taaagctcca tgacgaacgt
     5641 cttagcaccg tggaagcccg ttccggtctg tttgaacagg gcggctatcg ggcgctcaac
     5701 aaaggcaaag tcgactctgc ctctgcggtt attattctcg aaagctattt cgagcaggga
     5761 tattaaggcg atctaaaagc ctggcggata gtcgttaccg ccatcttgcc ggatgcggcg
     5821 tgaacgcctt atccagcaca catctggcag cggctgtagg tctgataaga cgcgttagcg
     5881 tcgcatcaga catttattgc ctttataggc cctataaact tgcgcatcgg gcatggcaac
     5941 gtcacaaacg cccttccccc acccgctgct gataactctg ctgaaacgtt aacatcccca
     6001 cctgctgccc ggtttgaata acatgcggta actggtgggt tttcccttcg cgaatcaaat
     6061 tccccaccgc gggagtgtta atcaataatt caaacagcgc cacgcgtcct tcctgtttat
     6121 ccacttccag cttttgtgac aacacggccc gtaaactacc tgccagttga ttacgtacgg
     6181 gatctttttc ctgcgccgga aatgaatcca ccagtcgctc aactgcctgc gcggcaccac
     6241 gcgtatgtaa tgttgccagc accaaatgcc cggtttctgc cgccgtcagt gccagacgga
     6301 ttgtctcgct gtcacgcagc tctccgagca aaatcacatc aggatcttcc cgcaatgcgg
     6361 cccgcaatcc cgatgcgaac gtcatacagt gcaaaccaat ttcccgctgc tggatcaaac
     6421 atcgctggct ggcatagata tattcaacag gatcttccag cgtcagaata tgcgcatcgg
     6481 catgttgata gagatagcca accatcgccg ctagcgtggt agatttgccg ctccccgtcg
     6541 cccccgtcac cagaatcagg ccattctcgc tcttgagtaa ttccggcaat accgttggtg
     6601 cgccaagctg ttcgagctgt ggacagtgcg aaggtaacaa ccgtaatgcc agagaaatac
     6661 cttgccgttg cgcgaacgca ctgccacgca accgctggtt ttccgccagc gacacggcaa
     6721 aatccagctg gccattttcc agcaatattg cccgctgatc gtcatccagc cactcccgca
     6781 gtagctcttc gacgtccggc gcatcaaacg gcgcagcttc cattagcccg cgaatgcgcc
     6841 atcgtgcgga ccaggcgctg cacaggtgta gatccgagac gttatgcttt acactaaggg
     6901 ccacaatttc ttccatattc atactaagat cctcggaaaa tgaacgatat tgcgcataac
     6961 ctggcacagg tccgggacaa aatctcagcg gctgcaacgc gttgcggccg ttctccagaa
     7021 gaaattacgc tgcttgcagt cagtaaaaca aaacctgcga gcgccatcgc agaagccatt
     7081 gatgccgggc agcgtcaatt tggtgaaaac tacgttcagg aaggggtaga taaaattcgc
     7141 cactttcagg aactgggcgt aacaggatta gaatggcatt ttattggccc gttgcagtct
     7201 aataaaagcc gcctggtggc agagcatttc gactggtgtc ataccatcga ccgtttgcgc
     7261 atcgctaccc gcctcaacga tcagcgcccg gcagaacttc cccctcttaa cgtactgatt
     7321 caaattaata ttagtgatga aaacagtaag tccgggattc aactggcaga actggacgag
     7381 ctggcagctg cggtcgctga actaccgcgt ttacgtctgc gcgggctgat ggcaatccct
     7441 gcacctgagt cagaatatgt aaggcagttt gaagttgcac gccaaatggc tgtagcattt
     7501 gccggactga aaacgcgcta cccgcatatc gacacgctct ctctgggaat gtcggacgat
     7561 atggaagccg ccattgcggc aggtagcacg atggttcgta tcggcactgc aatttttggt
     7621 gcgcgtgatt actctaaaaa ataaggaatt aaaggaacgc catgaatacg ttgactttcc
     7681 tgctttcaac ggtcattgag ctgtatacca tggtgctgtt attacgcatc tggatgcagt
     7741 gggctcattg tgatttttac aaccccttct cacagtttgt agtgaaggta acgcagccaa
     7801 ttatcgggcc actgcgccgc gttattccgg caatgggccc cattgacagc gcctcgctgc
     7861 tggttgccta tattctcagt tttatcaaag ccatcgtgct gtttaaagtg gtgaccttcc
     7921 tgccaatcat ctggattgcc ggtttactga ttctgctgaa aaccatcggc ctgctgattt
     7981 tctgggtcct gctggtgatg gcgattatga gctgggtaag ccagggtcgt agcccgattg
     8041 aatacgtgct gattcagctg gccgatccgc tgctgcgccc gattcgccgc ctgctaccgg
     8101 caatgggtgg gattgatttc tcgccgatga tcctcgttct gctgctgtat gtcatcaata
     8161 tgggtgtcgc agaagtatta caggcaaccg gaaacatgct gctgccgggg ctgtggatgg
     8221 cgttatgagt gccgtaacag ttaatgatga cggtctggtt ttacggctct atattcagcc
     8281 gaaagccagc cgtgattcta ttgtcggttt acatggcgac gaagttaaag tcgccattac
     8341 cgcgccgccg gttgacggtc aggccaacag tcatctggtg aagtttctcg gtaagcaatt
     8401 ccgggttgcc aaaagccagg tggtgattga aaaaggcgaa cttggccgcc acaaacaaat
     8461 taaaatcatt aatccgcaac aaatcccgcc agaaatcgcg gcgttaatta attaggtatc
     8521 ctatgcaaaa agttgtcctc gcaaccggca atgccggtaa agtgcgtgag ctggcgtcgc
     8581 tgcttagcga cttcggtctt gatatcgtgg cccaaacaga cctcggcgta gattccgctg
     8641 aagaaaccgg cctgaccttt atcgaaaacg cgattctgaa agcgcgccat gcggcaaaag
     8701 tgaccggttt accggcaatt gccgacgact ctggtctggc ggtagatgtg cttggcggcg
     8761 cgccgggcat ttactccgcg cgttattccg gtgaagacgc gaccgatcta aagaatctgc
     8821 aaaaactgct ggaaacactg aaagacgtac cggatgacca acgtcaggcg cgtttccact
     8881 gcgtgctggt atatctgcgt cacgcggaag atccgactcc gctggtatgc cacggtagct
     8941 ggccgggcgt gattactcgt gaaccagcgg gcactggtgg ctttggttat gatccaatct
     9001 tcttcgtacc ttccgaaggg aaaaccgccg ccgaactgac ccacgaagaa aagagcgcca
     9061 tttcccaccg tggtcaggca ttgaaactgc tgctggacgc tttacgtaat ggttaaatta
     9121 ccgccgctga gtctctacat tcacatcccg tggtgcgtgc agaaatgccc gtactgcgat
     9181 ttcaactctc acgcgttgaa aggagaagtg ccgcacgacg attacgttca gcatctgctt
     9241 tgcgatctgg acaacgatgt ggcttacgct cagggccgtg aagtaaagac aatctttatt
     9301 ggcggtggta cgccgagcct gctttccggc ccggcgatgc aaacgctgct ggacggcgtg
     9361 cgtgcgcgtt tgccgctggc agcggatgca gaaattacta tggaagcgaa ccctggtacg
     9421 gtagaagccg atcgctttgt cgattatcag cgtgctggtg tgaaccgcat ctctattggt
     9481 gtgcagagtt ttagcgaaga caagctgaaa cgacttgggc gtattcatgg cccgcaagaa
     9541 gcgaaacgcg cggcaaagct ggcgagcggg ctggggctgc gtagctttaa cctcgatttg
     9601 atgcatggac taccggatca atcactggaa gaggcgcttg gcgatctacg ccaggccatt
     9661 gaactgaatc cgccgcatct ttcctggtat caactgacca tcgaacctaa tacgctgttt
     9721 ggttcacgcc cgccggtact gccggacgac gacgcgctat gggatatatt cgaacagggg
     9781 catcagttat taaccgcagc gggttatcag caatatgaaa cttccgctta cgccaaatcc
     9841 agttatcagt gccagcacaa tctcaactac tggcgctttg gcgactacat cggtattggc
     9901 tgcggcgcgc acggcaaagt gaccttcccg gatgggcgca ttctgcgtac cactaaaacg
     9961 cgtcatccgc gtggttttat gcaaggaagg tatctggaaa gccagcgtga tgtcgaagcc
    10021 gcagataagc cgtttgagtt ctttatgaat cgcttccgtc tgctggaggc cgcgccgcgc
    10081 gtggagttta ttgcgtatac cgggctttgc gaagatgtga ttcgcccaca gttagacgag
    10141 gcgattgccc agggttatct caccgaatgt gcggattact ggcagataac ggaacatggt
    10201 aaattattct taaactcact tcttgaatta ttcctcgctg agtaatcatt aatgcgtctg
    10261 cgggcgcatt aatcacttct tccccccc
//
LOCUS       DAZFDP010000159        10138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFDP010000159 DAZFDP010000000
VERSION     DAZFDP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            199..702
                     /gene="ftnB"
                     /locus_tag="ACHJ1G_004171"
     CDS             199..702
                     /gene="ftnB"
                     /locus_tag="ACHJ1G_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4765930.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(781..1032)
                     /gene="yecJ"
                     /locus_tag="ACHJ1G_004172"
     CDS             complement(781..1032)
                     /gene="yecJ"
                     /locus_tag="ACHJ1G_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4765931.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1147..1233)
                     /gene="azuC"
                     /locus_tag="ACHJ1G_004173"
     CDS             complement(1147..1233)
                     /gene="azuC"
                     /locus_tag="ACHJ1G_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4765932.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1496..1819
                     /gene="yecR"
                     /locus_tag="ACHJ1G_004174"
     CDS             1496..1819
                     /gene="yecR"
                     /locus_tag="ACHJ1G_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4765933.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1990..2487
                     /gene="ftnA"
                     /locus_tag="ACHJ1G_004175"
     CDS             1990..2487
                     /gene="ftnA"
                     /locus_tag="ACHJ1G_004175"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4765934.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2525..2764)
                     /gene="yecH"
                     /locus_tag="ACHJ1G_004176"
     CDS             complement(2525..2764)
                     /gene="yecH"
                     /locus_tag="ACHJ1G_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4765935.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2955..4166
                     /gene="tyrP"
                     /locus_tag="ACHJ1G_004177"
     CDS             2955..4166
                     /gene="tyrP"
                     /locus_tag="ACHJ1G_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4765936.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4228..4893)
                     /gene="yecA"
                     /locus_tag="ACHJ1G_004178"
     CDS             complement(4228..4893)
                     /gene="yecA"
                     /locus_tag="ACHJ1G_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4765937.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5089..5175)
                     /locus_tag="ACHJ1G_004179"
     tRNA            complement(5089..5175)
                     /locus_tag="ACHJ1G_004179"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5139..5141),aa:Leu,seq:taa)
     gene            complement(5188..5261)
                     /locus_tag="ACHJ1G_004180"
     tRNA            complement(5188..5261)
                     /locus_tag="ACHJ1G_004180"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5227..5229),aa:Cys,seq:gca)
     gene            complement(5316..5391)
                     /locus_tag="ACHJ1G_004181"
     tRNA            complement(5316..5391)
                     /locus_tag="ACHJ1G_004181"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5356..5358),aa:Gly,seq:gcc)
     gene            complement(5543..6091)
                     /gene="pgsA"
                     /locus_tag="ACHJ1G_004182"
     CDS             complement(5543..6091)
                     /gene="pgsA"
                     /locus_tag="ACHJ1G_004182"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4765938.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6148..7980)
                     /gene="uvrC"
                     /locus_tag="ACHJ1G_004183"
     CDS             complement(6148..7980)
                     /gene="uvrC"
                     /locus_tag="ACHJ1G_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4765939.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7977..8633)
                     /gene="uvrY"
                     /locus_tag="ACHJ1G_004184"
     CDS             complement(7977..8633)
                     /gene="uvrY"
                     /locus_tag="ACHJ1G_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4765940.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8775..8891
                     /locus_tag="ACHJ1G_004185"
     CDS             8775..8891
                     /locus_tag="ACHJ1G_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4765941.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            8929..9105
                     /gene="yecU"
                     /locus_tag="ACHJ1G_004186"
     CDS             8929..9105
                     /gene="yecU"
                     /locus_tag="ACHJ1G_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4765942.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            9092..9316
                     /gene="yecF"
                     /locus_tag="ACHJ1G_004187"
     CDS             9092..9316
                     /gene="yecF"
                     /locus_tag="ACHJ1G_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4765943.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9384..10106)
                     /gene="sdiA"
                     /locus_tag="ACHJ1G_004188"
     CDS             complement(9384..10106)
                     /gene="sdiA"
                     /locus_tag="ACHJ1G_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4765944.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2552 a         2545 c         2338 g         2703 t
ORIGIN      
        1 aaatataaaa atatataaac ttgatgattt aagcattttc ttatacccgt tcagacgtta
       61 ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag
      121 gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg
      181 gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg
      241 aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag
      301 agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg
      361 atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat
      421 gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac
      481 gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat
      541 tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg tctgttgctg
      601 caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc
      661 gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc ggcgctaatg
      721 cattgcgccg atgaaggttt tgagaaaccg cagcctcatc tgtttgaagc agcggttttt
      781 ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg ctttttggat
      841 tgcctgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattcttcag
      901 ctgactggac attttttcac gaacttcaac gggcgcgatt acatcgagaa catccagaat
      961 ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag
     1021 cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac
     1081 atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt
     1141 tttttattat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
     1201 gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca
     1261 ggtgttattc tctttgcgct tataatatga caaccatcac aaaaatcaat ctttatgtga
     1321 tacaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa acagcccccc
     1381 tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt
     1441 gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct ggaccatgag
     1501 acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaggc
     1561 ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg accaggcttt
     1621 tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca
     1681 gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact gtagcctttt
     1741 tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc
     1801 cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga ttaaagaaat
     1861 agcggtaatg cgttttattc ccattcgtat ttttaataat tgaagtttat attttacctt
     1921 ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata acctttgtgg
     1981 agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat gaacctggaa
     2041 ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca taccttcgaa
     2101 ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg
     2161 tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga atctccgttt
     2221 gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga acaattaatc
     2281 acgcagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta cccaacattt
     2341 aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt caaatcgatt
     2401 attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa
     2461 ctctctaccc tcgacacaca aaactaatgc tctcaggcgg caggttttct gccgccgttt
     2521 caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa
     2581 atttgccttt tgctgccaga aacgctacca gctctcccgc agtcatccct tctgccgagc
     2641 aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat tgctgcttcc agactggcat
     2701 gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt
     2761 ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa gactttgcgc
     2821 cagcgcagga ctgaatgctt tttattgtac atttatattt acaccatatg taacgtcggt
     2881 ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata aagatcgtca
     2941 ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca
     3001 caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt tttagcgtta
     3061 cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg
     3121 aggtgtacca gcatgttccg gcagataccg gtcttggcac gctggcaaaa cgctatctgg
     3181 gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat gctctgactg
     3241 cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac tggacaggta
     3301 tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt
     3361 gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc aaaattattt
     3421 ttctagtggt gatgctggta ttgctgctac cgcatattca caaagtgaat cttttaaccc
     3481 tgccgttgca acaggggctg gctctgtcag caatcccggt gatttttacc tcatttggtt
     3541 ttcacggtag cgtgccgagt attgtcagct atatggatgg caacgttcgt aagctacgct
     3601 gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg caggtggcaa
     3661 cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat gctggattaa
     3721 acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag ctggcagtgc
     3781 atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta ggcttatttg
     3841 attatctggc tgatttattt cagcgttcaa ataccgttgg tggacggttg caaactggtg
     3901 caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga tttgtgatgg
     3961 cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct tcgctgttga
     4021 cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtgaaaggt ggtcgtccgg
     4081 cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg
     4141 cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt gggtgccaga
     4201 attaacgctg acacccaatt tgacctctta atgcaggcag cactgcttaa atttcttacc
     4261 actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga tcggttgctg
     4321 aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg ccgccagtcg
     4381 aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc gctcgaagtt
     4441 ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat cgggcaacgt
     4501 tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct caacaatcgt
     4561 cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct ccgggaattc
     4621 gttcagacgc tctgcggtat cggccatatg ttgaaaagcc agattcataa agcgcgtcat
     4681 ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg caaccagcca
     4741 ctgttccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac cgtccagctc
     4801 cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt ccagccattc
     4861 caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga tctcaactcg
     4921 ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg ctttgctggt
     4981 gattaaaaat tagggagggt gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt
     5041 ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg gtacccggag
     5101 cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg tgtctaccga
     5161 ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga
     5221 cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc aggcctttca
     5281 gccagacatc cgcttgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact
     5341 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa
     5401 gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg
     5461 ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt
     5521 gctgaaaatt acgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat
     5581 attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt
     5641 actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc tgggcagtgg
     5701 ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc
     5761 attcgcgtag cgcagaaata ataatttcac gggcgatcat cgttgccgcc ggtagggtca
     5821 cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg agaactttat
     5881 ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa
     5941 aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg
     6001 accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca
     6061 gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa
     6121 gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc
     6181 cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc
     6241 ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga cgccttcaat
     6301 ggtttccagg gagctggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc
     6361 gtgatcgtgt gattcatcgc gaatatgctg gataacatgc agcgcgggag aatctggcgg
     6421 caaactgaat ccctcacctt ccggctcaaa gaacagtgtt tccagcccag ccttacgatc
     6481 tactcctttg gcaacgccaa gtagcagtgg atgattttta tcccatgaga catccagttc
     6541 ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc
     6601 cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct gattcatcgc
     6661 cgcataatca tcgcccggcg tgatgcctgt aatgttatag cgccgatact ccgcacgcag
     6721 cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttcgccca tggtatggct
     6781 gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg caagcgcggt
     6841 cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt
     6901 ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga ggtttggttt gaacattaat
     6961 cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa
     7021 atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactggcc
     7081 tacgaaggtt tctaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct
     7141 gcggctgccg agcactttgc cctgacgaat gaacaatacg tggacacaag ccatgcccgc
     7201 atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta ttggaaacga attgtttttc
     7261 ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gcagcttctt caaactccag
     7321 attctggctg gcagtttcca tacgactaat gagttgcgta agcacctgat catctttgcc
     7381 agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc
     7441 ttcaacgcac ggtcccagac agcgccctat ctggtattgc agacacggac gcgagcgatt
     7501 gcgataaaca ctattttcgc actggcgaat ggggaaaatc ttttgcagta gcgccagtgt
     7561 ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttgg catgcttcgc
     7621 accacgatgc atcgccagac gcgggtgggt atcgccactc aggaagataa aaggataaga
     7681 tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc
     7741 cagcaacagc gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc
     7801 gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt
     7861 tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata
     7921 aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac
     7981 tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca
     8041 acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc
     8101 ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt gatcatcagc
     8161 ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg
     8221 atctggctta acgccatttg ttgagcgatg tcagaagcaa tgtaacgctg ccctgaatag
     8281 acagaacgaa tcgcactcac gacttcctgc ggagccgcgc ctttgctgag gtagcccgca
     8341 gcaccggcct gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg
     8401 attttgacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc
     8461 ggcatactca tgtccattag caccacgtca acggcatttg cccgacacca cttaacggcg
     8521 tcttcaccgc acgatgcctc accgacgact tttataccct ttatatcttc cagaatgcgt
     8581 cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata
     8641 tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag cggtttttat
     8701 tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtcaattc
     8761 gattgtccac agttatggtc acgcccgtca ccaacggctt accggatcgt ttcacagtaa
     8821 gccctgcgac gaaatgtgac aaaaattacc tttattcagc aaaaatgaaa atcagcccgc
     8881 tgaatatgta acattaatta acccgaacaa tacgggcaaa aaacattcat gataaaaata
     8941 tttattggtc attatattaa cgcatttaat agcaccgttg acgatacgct caaaaaacaa
     9001 ccactgctat ttttagcaaa gcttatggta tactccgccg ccttaacatt attcaccgca
     9061 aattttcatt gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga
     9121 gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct
     9181 gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta aagatggaaa aacagcttgc
     9241 gctgatcgaa gacgaaaccc aggctgcagt attttccaaa acggttaagc aaattaaaca
     9301 agcctaccgt cagtaatata aaaccggctg atagcgtgcc tttcagccgg tttttgcatc
     9361 tggcacgcag gacagaaaag agatcaaatt aagccagtag cggccgcgta acaggcaacc
     9421 tgggtcttat tcggtgcatt gatttttttc tgcatgtttt tctgatggaa attgaccgta
     9481 ttctcagaga ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccac
     9541 ctcagaattt ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca
     9601 tttaaacgca tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt
     9661 tcatcactaa gaatgggtat ttcgcgcgcg ctgcaacggg aaaaggacaa aaagcccagc
     9721 gcccggtttg gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg
     9781 gcttcccata acggctgtgc ttcgttgaat aagtcatcat tccacattag atggccctga
     9841 ctaaagtttt cagggttcag caccggatca attgcgagaa agttttttgc ctgataataa
     9901 ctaacccacg cctcagggta attggtgtag aaagccactt taggtcgcgt gaatggtacc
     9961 gggtggcgaa cacataacga atagtaatcg tactccagct gctgagcctg aagctctatt
    10021 tcatggtaga cctcttctgc ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc
    10081 cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg cccctgagag tgagtatc
//
LOCUS       DAZFDP010000160        10130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFDP010000160 DAZFDP010000000
VERSION     DAZFDP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            383..1153
                     /gene="yjjP"
                     /locus_tag="ACHJ1G_004189"
     CDS             383..1153
                     /gene="yjjP"
                     /locus_tag="ACHJ1G_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4765945.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1144..1617
                     /gene="yjjB"
                     /locus_tag="ACHJ1G_004190"
     CDS             1144..1617
                     /gene="yjjB"
                     /locus_tag="ACHJ1G_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4765946.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1724..2263
                     /gene="dnaT"
                     /locus_tag="ACHJ1G_004191"
     CDS             1724..2263
                     /gene="dnaT"
                     /locus_tag="ACHJ1G_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4765947.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2266..3003
                     /gene="dnaC"
                     /locus_tag="ACHJ1G_004192"
     CDS             2266..3003
                     /gene="dnaC"
                     /locus_tag="ACHJ1G_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4765948.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3052..3546
                     /gene="yjjA"
                     /locus_tag="ACHJ1G_004193"
     CDS             3052..3546
                     /gene="yjjA"
                     /locus_tag="ACHJ1G_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4765949.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3800..6091
                     /gene="opgB"
                     /locus_tag="ACHJ1G_004194"
     CDS             3800..6091
                     /gene="opgB"
                     /locus_tag="ACHJ1G_004194"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4765950.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6230..7252)
                     /gene="lgoD"
                     /locus_tag="ACHJ1G_004195"
     CDS             complement(6230..7252)
                     /gene="lgoD"
                     /locus_tag="ACHJ1G_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO4765951.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7391..8296
                     /locus_tag="ACHJ1G_004196"
     CDS             7391..8296
                     /locus_tag="ACHJ1G_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4765952.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8467..10119)
                     /locus_tag="ACHJ1G_004197"
     CDS             complement(8467..10119)
                     /locus_tag="ACHJ1G_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4765953.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2643 a         2604 c         2411 g         2472 t
ORIGIN      
        1 atacttaatg aaaaacagaa atcacattca tttaatttat aattaaatga tatcccttcc
       61 tgcagaatgc aaaatcacac aaccaatatt tattcaatga aatctgatgg ttttaacttg
      121 ttaacattaa aaaacgtgcc cggttatcta ttatcctgat tcgtaaaggc caaacaataa
      181 attcattatg atgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      241 ttatcaccga gcttctcccg ataaccagga ttaatcagcg caattttgat cttaacctac
      301 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      361 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cacggctttg
      421 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      481 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      541 cgccatagtg ctgacaacca ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      601 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      661 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg
      721 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      781 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
      841 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
      901 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcgatacacc
      961 caccattgcg atggccgcca gcgttctgct gctggtgccg ggctttccgt tgattaatgc
     1021 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1081 tctgctgacg ctggctacct gcgtcggcgt ggtgatggca ctgacgattt gggggctacg
     1141 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1201 cctgcggtcg gctttgcgat ggtgttcaac gttcccgttc gggcgttacg ctggtgtgcg
     1261 ctgcttggcg cgattggtca tggttcacga atgatcttaa tgaccagcgg gctgaatatt
     1321 gagtggtcaa cttttatggc ttctatgctg gtcggcacca ttggtattca atggtcgcgc
     1381 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1441 atatcggctt ataccgcaat gatttcggcg gtaaaaatca gccagttagg ttacagcgaa
     1501 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1561 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1621 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1681 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1741 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1801 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     1861 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga
     1921 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtccccat
     1981 ggggaaattc gccatgtatc cggactggca acccgatgcc gactttatcc gcctggcggc
     2041 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2101 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2161 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2221 cagcgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2281 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2341 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2401 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2461 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2521 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2581 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2641 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2701 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2761 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     2821 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     2881 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     2941 agtttgtggg tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat
     3001 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3061 actgttaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3121 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3181 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg
     3241 aaccaggcct taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg
     3301 aagcaaaagc tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3361 ctgggcctga acagtgaaga acaaaaagaa gacaccaact atctggatgg cattcagggt
     3421 ttgctgaaaa ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3481 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3541 tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc
     3601 cttttccctg ctcctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa
     3661 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     3721 atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg
     3781 gtttagtgag gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt
     3841 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt
     3901 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcgc
     3961 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     4021 caaatacatc ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct
     4081 gggctggatc ctgcgccgtc gtcgccatca tccgcaccat tttggttaca gcctgctggc
     4141 gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     4201 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     4261 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     4321 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     4381 tgaaggcctg gatttcagcc acacgcagca gctgccggga acagattaca cgattgcggg
     4441 catggtggct tctcagtgcg gcattccgct ttttgcccca tttgaaggta acgcctccgc
     4501 ttctgtttcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     4561 ttatcagaac tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct
     4621 gaagtcgcat ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtagccga
     4681 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     4741 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     4801 tacccatcac ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga
     4861 cggtaagccg aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt
     4921 tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg tctcttctga
     4981 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     5041 gttttttgtc attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac
     5101 gatggataac ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg
     5161 tcgtagcagt ttatccgggc agtcaatgtc ggaaatcttc ctcaatatca aagagaaaac
     5221 attggcgtgg aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     5281 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     5341 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc
     5401 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg
     5461 ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact ggtgtgtctc
     5521 gcaggggcag cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa catggcaggg
     5581 caaaacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca atgtcgatac
     5641 gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc
     5701 cggtgcgcca gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg
     5761 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa
     5821 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     5881 ggtacgcgta ggcaatgaag aacaaaccct tgtgctgggc aatgaagtga ccaccaccac
     5941 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     6001 ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt
     6061 ggaaattaaa gttgtggaac gtgaagggta attcctgact gatgtgtagg tcgcataagg
     6121 tgcgacctac acaccttctc cggcctacaa aacatcattg agaaacagaa aaaaacagcg
     6181 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6241 ttacaccttt aattaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6301 tggcgaacgg atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca
     6361 gtcgaccgac tttagcaaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6421 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata
     6481 ccaccgtgcc gccgtgacga atcagattca cggtgttatt catcgcatgt tgattacctg
     6541 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcaac tgcgcgtcaa
     6601 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6661 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6721 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6781 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     6841 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     6901 catcctgatg cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac
     6961 acgccgggca ttgctgacaa gaaatatacg gaattaccgc aacttgctgt ccgatattaa
     7021 aattgtgaaa gttttttcct gattcaataa tctccccaca tatttcatgg cctaaaacgc
     7081 gtggatagct aaaaaatggt tgattgcccg cccaggcatg aatatctgta ccgcaaatcc
     7141 cgacggtttt aattttaatt aatgcttcat tatcgcctgg caccggtctt tcccggactg
     7201 tccatactaa attctttatt tcctgacaaa ttaatacttt cattgtggac atgattcacc
     7261 gctcctttta ttttatgact ttcagatatc gtcgaattta taagagcacc tgaatttacc
     7321 cataaaatat gcggatgatc acgcataaaa atgttttaaa tcggttttta atgccggact
     7381 ggagggaaga atgagtcgtt cgcagaattt acgtcataac gtcataaatc aggtgattga
     7441 agatatggcg aggggcaata ttccttctcc gctgccttca caaagtgggc tggcagaaat
     7501 gtataacatc agtcgcacta cggtacgcca catactccag catttaagcg catgtggcgt
     7561 gctcaccctg gtggggaaaa attatgttat cgcccgcaaa cccgaacaga acgatggatt
     7621 tgattgcatc acggcctcgc tgaaagagca aaaccgtctt ttcgagcagg catttttcac
     7681 catgattaac ctgcgccagt tacgcgcggg agaaagcttt tccgaacttc agctggcgcg
     7741 agcggcaggc gtcagtccgg tagtagtacg agaatatctt ttgaaattcg gtcgttataa
     7801 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag cagttcgatc aatcctacgc
     7861 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tccctgcaac actttctcaa
     7921 ccttcccgat gacgatcccc gctggttgca ggcgaaaacc atgctcgaac gtcacaggac
     7981 attgcgcgac agcattggca gcaactttcg gatgttttct caattagacc gtgattttca
     8041 tgccatgctg ttatccgctg ctgacaacat cttttttaat caatcacttg aaattatctc
     8101 ggtgatcttt cattttcact atcaatggga tgaaagcgat ctcaagcagc gcaatatcat
     8161 tgctgttgat gagcatatga ccattctcag cgccctgatt tgccgtagcg atttagatgc
     8221 aataacggca ttgcgaaacc atctggatac cgccaaacaa tcaatgattc gttccatcag
     8281 gcaacatcac cgataaatta agtacagatt aaaaacattg attcttggtt tatatcacta
     8341 tgttgtatta atgcagtatt aatccaggcg attgttctgc ccataaggaa atacataggg
     8401 aataaacgat gctggaagta ttactctcag ccatcattta actccctatt actggtagcg
     8461 ataaaattat cggctatcca tgattaacgt ttccagcaca tctaattcac tgactatttt
     8521 tgccagtgtt tcatcactga cttcttgccg tgcacgcatc tggtaaatag ccgcacgcct
     8581 ggcatttaac gctgccagat taaatgttct ttccagcttc gcgcgctgca tatgtaaaga
     8641 attatcattt tccgacatat catcttcgct acgccgcata taaccgataa ctctggcccc
     8701 tgtttccata atcaactcgt tgtcgaggtt ttctgtactg gtgcgtagca aacgttgctc
     8761 agtcttttgc acactgagta tggccatttt ggccataaac agatcggcag cttcctcttc
     8821 tttttgccgg ttatcgctga aaatgcccac atttttcccc agtagtaacg gcagaataat
     8881 aatgccgcaa accagcgaaa agaggatcac acctgtggcg agaaatacca gtaagtaacg
     8941 cccgggaaaa ggtgtgccat ccggcagcaa taacgggata gaaagcaccc ccgcaagcgt
     9001 gatggctccg cgcacccccg caaaggtcgc cagcagcata tgacgcgttt tatatccagc
     9061 aaacagcatc ggatgtttgc cgggtcgttt acggctatac agacgcatca gccaaagcca
     9121 gcccaggcgc acaaccatta acgccaggta gatccaaatg acatcaagga gcagcgatcc
     9181 ggtgtctacc agtggatcgt tttctgcacg gatcagtgat tccgaaataa tgcccggcag
     9241 ttgtaagcct aaaatcagaa acaccatgcc gttaaagaca tattcaagca tcgaccagct
     9301 acttttagct ttaattctca ggcggatagg cgcgtggtgg actacgccaa tgcgactggt
     9361 tgttaacccc gcggcgacag cggcaagaat gccagataag ccaaaatgtt ccgcaaccag
     9421 ataaacaata aacggcaaca gaagcatcaa tacaatctga atggaaggtt catcgtgagc
     9481 atatcgactg atcaacagca gccctttgcc atataaccag gtaacagcaa tccccacggc
     9541 aataccgccc aacgcaacga caaaaaacgc aatggttgcg ccatgaacgc tgaattccat
     9601 cgttcccatc gtcactgcaa cagcaaattt caacgccacc aggccggaag catcattcat
     9661 caacgcttcg ccttcgagca cttcctggat gtttttgctg atacgccctt tcccgacaat
     9721 ccccagcaac gccacggcat cggtaggcga caacacggcg gctaatgcga aagcagcgat
     9781 gagcggcaca ttcggtaaga gtaaatgaat cacatagccg acaccaaaaa tagtgattag
     9841 caccaacaca agcgccaggc tgataatttc ccgactgtgc ttgatcaatt ctttaggttg
     9901 tatttttgag ctttcagcaa acaacaaggg gggtataaac aataagagaa aaagttcagg
     9961 attgaattcc acatgcagac caaatatggg ctgggccagt acagcgccag cggcaatctg
    10021 gatcaaagga agtggtatct gaacaggaga cagacgcgta agcacgctgg ttaatgatac
    10081 taccaacgta agaattaaaa ttgttaaaag tatttccata taataaacag
//
LOCUS       DAZFDP010000161        10110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFDP010000161 DAZFDP010000000
VERSION     DAZFDP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            187..5363
                     /locus_tag="ACHJ1G_004198"
                     /pseudo
     CDS             187..5363
                     /locus_tag="ACHJ1G_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5586..5852
                     /gene="hipB"
                     /locus_tag="ACHJ1G_004199"
     CDS             5586..5852
                     /gene="hipB"
                     /locus_tag="ACHJ1G_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO4765954.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5852..7174
                     /gene="hipA"
                     /locus_tag="ACHJ1G_004200"
     CDS             5852..7174
                     /gene="hipA"
                     /locus_tag="ACHJ1G_004200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO4765955.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7486..7694
                     /locus_tag="ACHJ1G_004201"
                     /pseudo
     CDS             7486..7694
                     /locus_tag="ACHJ1G_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8126..8689
                     /gene="fimA"
                     /locus_tag="ACHJ1G_004202"
     CDS             8126..8689
                     /gene="fimA"
                     /locus_tag="ACHJ1G_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4765956.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9050..9760
                     /locus_tag="ACHJ1G_004203"
     CDS             9050..9760
                     /locus_tag="ACHJ1G_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4765957.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9802..>10110
                     /locus_tag="ACHJ1G_004204"
     CDS             9802..>10110
                     /locus_tag="ACHJ1G_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFO4765958.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     C"
BASE COUNT         2814 a         2153 c         2595 g         2548 t
ORIGIN      
        1 aataaaataa gaactatgca ttttttaatc tatcaattcg aaagtattag caaattagcc
       61 aaattgacac cttcccacct ccaactaaca ctcaatacag acctctgaca atttaataag
      121 tttcattttt aatgtctgac gactcatctc tttcgtggct gtattttttg tatatggagc
      181 gtttatatga atagaatcta tcgcgtgata tggaattgca ctctacaggt atttcaggcc
      241 tgctcggaat taactcgcag ggcaggtaaa acatcgacgg ttaatttgcg taaatcctct
      301 ggactgacaa cgaaattcag tagattgacg ctgggtgttt tgctggcact aagcggttca
      361 gcgtctggtg caagtctgga agttgataat gatcagatta ccaatattga tactgatgtt
      421 gcttatgatg cctacctggt tggctggtat ggcactggag tgcttaatat tttggctggc
      481 ggtaatgcct ccttaaccac tattactacc agcgtcattg gcgctaatga ggactcagag
      541 ggcaccgtta atgttttggg tggcacctgg cgattgtatg atagcggaaa taatgcaagg
      601 cctttaaatg tgggtcaatc cggaacgggg acgctgaata ttaaacagaa gggtcacgtc
      661 gatggaggct atttaagatt aggttcttcg acaggaggcg tcgggacggt caatgttgag
      721 ggagaggact ctgttctgac gaccgaatta ttcgaaatag ggagttacgg tacgggctca
      781 ttaaatatta cggataaggg ttacgtcacg agttcaatcg tcgccatttt aggctatcag
      841 gcgggcagta atggccaggt tgtcgttgaa aagggtggcg agtggctaat aaaaaataac
      901 gattcctcaa ttgaatttca aattggtaat caaggaactg gggaggcgac tattcgcgag
      961 ggtggactga ttacggctga aaatacgatt atcggtggca atgccaccgg tgtcggaacc
     1021 ctgaatgtgc aggatcaaga ctctgtcatc acggtacgca gactctataa tggatatttc
     1081 ggtaatggca cactcaatat ttccaataat ggactaatta ataacaaaga atattcatta
     1141 gtgggcgttc aggacggttc ccacggtgtc gtcaacgtga ccgataaagg gcattggaat
     1201 ttcctcggaa cgggcgaagc tttccgctat atctatatcg gtgatgctgg cgacggtgaa
     1261 cttaatgtct cgcgtgaagg taaagtagat tcgggaatta tcactgcggg gatgaaagaa
     1321 acaggcacag gcaacattac tgttaaggat aagaactccg ttatcactaa tctcggaact
     1381 aatcttggtt atgacggcca cggcgaaatg aatatcagta atgaagggct tgttgtcagc
     1441 aacggaggaa gctcactcgg ttatggagaa accggcgtcg ggaatgtcag catcaccacg
     1501 ggggggatgt gggaggtcaa taagaatgtc tataccacca ttggtgttgc gggcgtcgga
     1561 aacctcaata ttagcgatgg cggtaagttc gtatcgcaaa atattacttt tttgggcgat
     1621 aaagcaagcg gtatcggcac actgaacctg atggatgcga catcatcgtt cgatactgtg
     1681 ggtatcaatg tcggtaattt tggtagcggt atcgtaaatg tcagtaatgg tgccaccctt
     1741 aattcaacgg gctatggatt tatcggagga aatgcctccg gtaaggggat agttaatatt
     1801 tcaacggaca gtctctggaa tttaaagaca tcatctacta acgcacaatt gctacaggtc
     1861 ggtgtattag gcaagggtga actgaatatt accaccggag gtatagttaa agcgcgtgat
     1921 acacagatag ctctcaatga caaaagtaag ggcgacgtga gggtggatgg gcagaactct
     1981 cttcttgaaa cattcaatat gtacgtaggg acatctggta cgggtacgtt aaccctgacg
     2041 aatagcggca cgctgaatgt cgaaggtgga gaagtttact taggtgtttt tgaacctgct
     2101 gtaggaacgc taaacattgg tgcagctcac ggtgaggcgg cggcagatgc cgggtatata
     2161 accaacgcga cgaaagtaga gtttggttct ggcgaaggtg tttttgtctt taatcatact
     2221 aataacagtg atgccggcta ccaggtcgat atgctgatta caggtgacga taaagacgga
     2281 aaagtgatcc atgatgcagg ccatacggtg ttcaatgcag ggaatactta tagcggtaaa
     2341 acgctggtca atgacggcct cctgaccatt gcgtctcata cggcagatgg ggtaacgggc
     2401 atgggttcga gtgaagtcac cattgcaagc cccggtacgc tcgacattct cgcatcaacg
     2461 aacagtgcag gagattacac gctgaccaat gcgctcaaag gcgatggctt gatgcgagtg
     2521 cagctgtcat cctacgacaa gatgtttggc tttacccatg caacagggac tgaattcgcc
     2581 ggtgttgccc aactgaaaga cagtaccttc actctggaac gcgacaacac cgctgcgctt
     2641 actcacgcga tgttgcagtc tgacagtgaa aataccacat cggtaaaagt gggagagcaa
     2701 tccattggtg gactggccat gaatggcggt acgctcattt tcgatacgga tattcctgct
     2761 gcgacgcttg cagagggata tatcagcgtc gatacgctgg ttgtcggcgc gggtgactac
     2821 acctggaaag gccgtaacta tcaggtaaac gggacgggcg acgtgcctaa accgtggaat
     2881 gatcccatgg cgaataaccc tctgacgacg ctcaatttgc tggaacacga tgatagccat
     2941 gtcggcgttc aactggtgaa ggcgcaaacg gttattgggt cgggtggctc attaacgtta
     3001 cgtgatttac agggcgacga ggtggaagcg gacaaaacgt tacacattgc gcaaaacgga
     3061 acggtggtcg ccgagggtga ttatggattc cgcctcacga ccgcaccagg tgatggtttg
     3121 tacgttaact atgggctgaa agcgctgaac atccatggtg ggcaaaagct gacgttagcc
     3181 gaacatggcg gagcctatgg cgcaacggcc gatatgtcgg caaaaatcgg tggtgaaggg
     3241 gatctggcaa tcaatacggt gcgacaggtt tcgctttcca acggtcagaa cgactatcag
     3301 ggggcaacct acgttcagat ggggacatta cgtaccgatg cggatggtgc acttggcaac
     3361 acccgggaac tgaacatcag caacgcggcc atcgtcgatc ttaatggatc gacgcagacg
     3421 gtagagacat tcaccgggca gatgggttcg actgttttgt tcaaagaggg ggcgctgacg
     3481 gtaaataaag gtgggatcag tcagggtgaa ctgacaggtg gcggaaacct gaatgttaca
     3541 gggggaacgc tggctatcga ggggcttaat gcacgctaca atgcgttaac cagcattagc
     3601 ccaaatgcgg aagtcagcct cgataatacg caggggttag gcagaggaaa tattgccaat
     3661 aacggtctgt taacgctaaa aaacgtgact ggcgaactgc gcaatagcat aagcgggaag
     3721 ggtatcgtga gcgcaaccgc caggacagat gtagagctgg atggcgataa tagccgcttt
     3781 gtggggcaat tcaacattga tacaggcagc gcgctcagcg tcaacgagca gaaaaacctg
     3841 ggtgatgctt ccgttatcaa taatggcctg ctcaccatct ccactgagcg tagctgggcg
     3901 atgacgcaca gtatcagcgg tagcggtgat gtgacaaaac tgggtaccgg gatcctgact
     3961 cttaacaacg attccgcggc gtatcagggt acgacggata tcgtgggggg gggaaattgc
     4021 tttcggttcc gactctgcca ttaatatggc aagtcaacac attaatatcc ataacagcgg
     4081 tgtgatgtcg ggaaatgtca ccactgcagg tgatgtgaac gttatgcctg gggggacact
     4141 gcgtgtcgct aaaaccacaa tcggcggcaa cctggagaat ggcggcacgg ttcaaatgaa
     4201 tagcgaaggg gggaaaccgg ggaatgtact gaccgttaac ggcaactata ccggaaacaa
     4261 tggcctgatg acgttcaacg cgacgctggg cggcgataat tcgcccaccg ataaaatgaa
     4321 cgtgaaaggc gatacccaag ggaacactcg cgttcgggtt gataacattg gcggcgtcgg
     4381 tgcacaaacg gtcaacggta ttgaactcat tgaggttggc ggtaattctg caggtaactt
     4441 cgcgctgacc accggaactg tcgaagctgg ggcttacgtc tacacgctgg ctaaagggaa
     4501 ggggaatgac gagaaaaact ggtatctgac cagtaaatgg gacggcgtaa cgccagcgga
     4561 tacacccgat cccatcaata atccccctgt tgtggatccg gaaggcccat cagtttatcg
     4621 cccggaggcc ggaagctata tcagcaacat tgccgcagcc aactcgctgt ttagccatcg
     4681 tttacacgac cgtctgggtg agccgcagta tatagattca ctgcattctc aggggtcggc
     4741 aagcagtatg tggatgcgtc atgtcggagg gcacgaacgt tcaagggccg gtgacggtca
     4801 gctaattact caggctaacc gctatgtatt gcagctaggc ggcgatttgg cgcagtggag
     4861 tagcaacgcg caggatcgct ggcatgacgg cctctgttga gggtgggtat acctttgaag
     4921 cgggaacatt tagaggcagc gaagggacgc tgaatacctg gtacgtccag ccacaggcgc
     4981 aaatcacctg gatgggtgtg aaagattctg accataatcg gaaagacgga acacgcattg
     5041 aaacggaagg cgacggaaac gtgcaaacgc gacttggggt gaaaacctac ctgaatagcc
     5101 atcacaagcg tgacgatggt aaacagcgtg agttccagcc ttacattgaa gcgaactgga
     5161 tcaacaatag caaagtctac gccgtgaaga tgaatggtca aaccgtaagc cgtgatggtg
     5221 cgcgtaatct cggtgaagta cgtaccgggg ttgaggcgaa agtaaataac aaccttagcc
     5281 tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta tagcgatact cagggcatgc
     5341 tgggagtgaa atatagctgg taaaccgtat aagccgcatg tcgagatggc atgcggctta
     5401 atattgccga cttcaaacgg cgcatcagcg ccttttttaa atcctccttt ttatccgcga
     5461 tcgcggatat cgcagcgttt atcccgtaga gcggataaga cgtgtttcca aattgactta
     5521 tcctcactaa aggataaaac tgataatatc ctcttatgcg gataaatttg ctgtggacgt
     5581 atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat gcaatgaaac
     5641 tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt ggcattaagc
     5701 aggcgacaat ttccaatttc gaaaacaacc ctgacaatac cacgctcacg acatttttta
     5761 agattttaca gtcgcttgaa ctctcaatga cactatgcga cacgaaaaat gcctcgccag
     5821 aatcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt ggatgaacaa
     5881 ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta agtatgcacc
     5941 ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat tgcagagggg
     6001 gaatatcacc tcagatgccg tatttaactt cttcgataac ctgttacccg atagcccgat
     6061 tgtacgtgac cggatcgtta aacgttatca tgctaaatcc agacaaccgt ttgatttatt
     6121 gtcagaaata gggcgagaca gcgttggtgc cgtaacgtta ttacccgaaa atgaaaccat
     6181 aacgcgcccg ataatggcat gggaaaagct tactgaagcc aggcttgaag aagtattaac
     6241 ggcttataaa gcagatatcc ctctgggcat gattagagaa gaaaatgact ttcgcatctc
     6301 ggttgctggc gcgcaggaga agacagcgct gcttagaata ggcaatgact ggtgcattcc
     6361 gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa tcagacagcc
     6421 caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc tgctgctggc
     6481 gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa atgtgcgcgc
     6541 gttagcggtc gaacgttttg acaggcgttg gaatactgag cgaacggttt tacttcgctt
     6601 gccacaggag gatatgtgtc agacattcgg tttaccttca tctgtgaaat atgaatcaga
     6661 tggaggccca ggcatcgcgc ggatcatggc gtttttgatg gggtccagcg aggcgcttcg
     6721 cgatcgttat gattttatga aattccaggt cttccagtgg ttgattggcg caacggatgg
     6781 ccatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac tcacgccatt
     6841 ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaatacaca tcagcgatct
     6901 caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg ataaaattta
     6961 tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc agatgcatga
     7021 aatcctaagt gactttgcca gaatgattcc ggcagcactg gataacgtga agacttcatt
     7081 accgacagat tttccggaga acgtggtgac ggcagttgaa accaatgtgt tgaggttgca
     7141 tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc gttgttaatg
     7201 agcacgacaa tcatgaccgc caacgactgg attcttcctt ggtttatact ggtgcgcata
     7261 atgtatatta tgttaaatta tgtctgagtg atgcgaagtg cttttctggt agtcgttatt
     7321 cgttcagggt tcaagaactt aagcctctcc tctttcataa ttggcctgtg catggagaaa
     7381 tagcctggtt attccctgcc ccatgctaag agcgcagtgt ctgtcgcata gacactgcat
     7441 acgaaaacac cagagggtgc tgttacttat ttaaggacgg atcaagcggg aatgagtcca
     7501 ctccggtatc agaaatggct acgtctcaat gaagtcaggc gaccgatgct gaatgaacat
     7561 tacgatgtca ccacagcagc ctatgctgtc ggttacgaaa gctatcccat ttcagtcggg
     7621 aatattcgcg gatgtttgga gagtcaccca agagagatat taccaggtta agaaagtctg
     7681 tcggtcagct ttaataaacc ctgcacttat ctgtttagag caattcggtg ttagtttcag
     7741 caagcaaaca ttaaccatag ctaatgattt atagccatat taaccattgg tagcccttga
     7801 cctttcctga cacttccctt gttcgcaata atattctgtt tagtcacggt ttataaataa
     7861 catgcataat gaaatcgtca tgttatttag cgacataaaa tgtttttgca aatactctct
     7921 tcaacaagtg cacaataaac ttgttgtgat taggtgtcct tgtgatttaa agatattatc
     7981 gttcttcgtg aaatgtaata accctggaaa ctatattctc agggcaacaa ccaatcaaga
     8041 atgattcttg tttttaagca ataacagaat gacttaattg ttaaccctct ttattcagct
     8101 acttaaatat aaattttgga gaattatgaa actcaaacat gttggtatga ttgtcgtttc
     8161 tgtgttggcg atgtcgtctg ctgcggtaag cgcagccgag ggtgatgaat cagtaacgac
     8221 cactgttaat ggcggtgtta ttcattttaa aggtgaagtg gtaaatgccg cttgtgcaat
     8281 tgattcagaa tcaatgaacc aaacggttga gctgggtcag gttcgttctt ctcgcttggc
     8341 taaagcgggt gacctcagct ccgccgttgg cttcaatatc aagctgaatg attgtgatac
     8401 caatgtttcc agtaatgcag ctgttgcatt cctgggtact actgtcacca gtaatgacga
     8461 tacgttagcg ctgcagagtt cagcggcagg ctctgcccaa aatgtcggta ttcaaatttt
     8521 ggaccgtacg ggtgaggtat taatacttga tggggccact tttagtgcta aaaccgactt
     8581 gattgatggc acgaatatac tgccattcca ggctcgttat attgctctcg ggcagtccgt
     8641 agctggtact gcaaacgctg atgcgacctt caaagttcaa tatctataat tcaatcttaa
     8701 ggtggctata cgtcatctga aattgaaaca gggtgtgtgt ggcatggcta cgcacacccg
     8761 agcaatttac caatattcat ctcattttta atgagaaatg gtttgtattt ggagcgctat
     8821 gaaaaatcac ataatatcta aactatcaat aaacaacagt agttaaactt actgttattt
     8881 aaaatattta ctttgtagca aggttttcct attaaaagag aaccaacatt tattacaaac
     8941 gcattaagtc tggaaacccg gcgactcgcc aatgtattag caacattaat cgattgtctg
     9001 atgtaacggt tttcccgctt atcctcttaa cacatggaga atgcatcaga tgcaaactac
     9061 gcgtaccccc tactctattt cctttatggc gacagtgtta cttttattgc tctttgcttg
     9121 ccatagcacc gttgccaacg cagctgtcgc actgggtgcg acgcgggtaa tttatccggc
     9181 aaaccagaaa caggttttat tgcctgtcac gaataatgat cctgccagtg tctatctcat
     9241 tcagtcatgg atagaaaatg caggtgacca aaaagacacc cagtttgtca ttaccccacc
     9301 gcttttttcc atgcaaggta aaaaagagaa tacgctgcgg ataatcaacg ctactaatca
     9361 tcagttacca ggcgaccgtg aaagcctgtt ctgggtgaat gttaaagcta ttcctgctat
     9421 ggaaaaagac cagaagaatg agaatacatt acagctggca attattagcc gcataaaaat
     9481 gttttatcgc cccacgaacc tggcaatggc acctgaagaa gcgcctgcaa tgcttcggtt
     9541 tcgtcgcagc ggtagcaaac tcacgcttat taatccaacg ccctatttca ttaccgtaac
     9601 aaatatgaaa gcaggaaata gcaatctccc aaacaccatg gtgccgccaa aaggtgaagt
     9661 atcggtggat atcacgcatg ctgcaaccgg ggatatcagt tttcagacga tcaatgatta
     9721 cggtgcatta actccgcgta ttaaggcaac catgcagtaa gccagtgacc taattaataa
     9781 taaactcaat cggaattaat aatgacagcc tttcgtgccg catttaaggc atatcgtatg
     9841 catcaggtgt taataatgcc tcgctttgca cggcttacca ttgcccttgg cttagcaact
     9901 gcggtatttc ccgttgacgc agagtattat tttaatccac ggtttttaag caatgatctt
     9961 gctgaatctg tcgatttatc cgcctttact aagggacgcg aggcaccacc tggtacatat
    10021 cgggtcgaca tttatttgaa cgacgaattt atgactagtc gggatattac ttttattgca
    10081 gatgataata acgcagaact catcccatgc
//
LOCUS       DAZFDP010000162        10097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFDP010000162 DAZFDP010000000
VERSION     DAZFDP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHJ1G_004205"
     CDS             complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHJ1G_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4765959.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(926..2356)
                     /locus_tag="ACHJ1G_004206"
     CDS             complement(926..2356)
                     /locus_tag="ACHJ1G_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4765960.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2635..3588
                     /gene="tal"
                     /locus_tag="ACHJ1G_004207"
     CDS             2635..3588
                     /gene="tal"
                     /locus_tag="ACHJ1G_004207"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4765961.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3703..4290
                     /gene="mog"
                     /locus_tag="ACHJ1G_004208"
     CDS             3703..4290
                     /gene="mog"
                     /locus_tag="ACHJ1G_004208"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4765962.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHJ1G_004209"
     CDS             complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHJ1G_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4765963.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHJ1G_004210"
     CDS             complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHJ1G_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4765964.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5779..6183)
                     /locus_tag="ACHJ1G_004211"
     CDS             complement(5779..6183)
                     /locus_tag="ACHJ1G_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4765965.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHJ1G_004212"
     CDS             6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHJ1G_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4765966.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHJ1G_004213"
     CDS             8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHJ1G_004213"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4765967.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHJ1G_004214"
     CDS             complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHJ1G_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4765968.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2644 a         2534 c         2627 g         2292 t
ORIGIN      
        1 aattctgcaa tatattgaat ctgcatgctt ttgtaggccg gataaggcgt tcacgccgca
       61 tccggcattg actgcaaact taacgctgct cgtagcgttt aaacaccagt tcgccattgc
      121 tggaggaagc ttcatcaaag aagtaacctt cgctattaaa accagtcagt tgctctggtt
      181 tggtcagccg attttcaata ataaaacgac tcatcagacc gcgtgctttc ttagcgtaga
      241 agctgattat cttaaatttg ccgttcttct catcgaggaa caccggcttg ataatctcgg
      301 cattcaattt cttcggcttc accgatttaa aatactcatc tgacgccaga ttaatcacca
      361 cattatcgcc ttgtgctgcg agcgcctcgt tcagcttgtt ggtgatgata tctccccaga
      421 attgatacag atctttccct cgggcattct caagacggat ccccatttcc agacgataag
      481 gctgcattaa atcgagcggg cgcagtacgc catacaagcc ggaaagcatt cgcaaatgct
      541 gttgggcaaa atcgaaatcg tcttcgctga aggtttcggc ctgcaagccg gtgtagacat
      601 cacctttaaa cgccagaatc gcctggcggg cattctccgg cgtgaaatct ggctgccagt
      661 catgaaagcg agcggcgttg atacccgcca gtttgtcgct gatgcgcatc agcgtgctaa
      721 tctgcggagg cgtcagtttc cgcgcttcat ggatcaactg ctgggaattg tctaacagct
      781 ccggcagcgt atagcgcgtg gtggtcaacg ggctttggta atcaagcgtt ttcgcaggtg
      841 aaataagaat cagcatatcc agtccttgca ggaaatttat gccgacttta gcaaaaaaag
      901 agaatgagtt gatcgatagt tgtgattact cctgcgaaac atcatcccac gcgtccggag
      961 aaagctggcg gccgatatcc ggataacgca acggatcaaa caccgggcgc acgccgagtt
     1021 tacgctggcg tagataatca ctggcaatgg tatgaaccac aggcgagagc agtaaaatgg
     1081 cggtcaaatt ggtaatagcc atgcaggcca ttatgatatc tgccagttgc cacatcagcg
     1141 gaagacttag caaggtgccg ccgatgaccg ttgcgaaggt gcagatccgc aaacaccaga
     1201 tcgctttagg gttgttcagg cgtaaaaaga agagattgtt ttcggcgtaa atgtagttgg
     1261 caacgatgga gctgaaggca aacagaataa ccacgagggt aacaaactca gcaccccagg
     1321 aacccattag cacccgcatc gccttctgga taagctgaat accttccagc ggcatgtagg
     1381 ttgtgccgtt acccgccagt aatatcagca tggcgcttgc cgtacagatg accagggtgt
     1441 cgataaaaat gccaatcatc tggacaatcc cttgcgctgc cggatgcgga ggccaggacg
     1501 ccgctgccgc tgccgcgttt ggcgtcgacc ccattcccgc ctcattggaa aacatactgc
     1561 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
     1621 cctgccagcc aaaagcactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
     1681 tcattacgca aatcaccagg ctggtcagta cccagattat cgccatcaac gggacaaacc
     1741 cctgcatgag ccgggcgacg ccatgaagac cgcgagtgat cgccagcaga gcaaagacag
     1801 cgagaataat acctgtcacc agcgggggaa aatcaaaaga aaaactcagg gctcgcgcaa
     1861 cggcgttcgc ctgaattccg ctgaaaatta tgccgtaggc gatgagcaaa aagacggcga
     1921 acagaacgcc catccagcgc atccccagcc cgcgcgccat ataccatgcc ggtccgccac
     1981 gaaactgccc attggcatca cgttctttat aaagctgtgc gagggaacat tcggcaaacg
     2041 aggtcgccat gccgataaac gcggcaaccc acatccagaa gacggctcca ggtccaccgg
     2101 cggtaattgc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
     2161 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
     2221 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
     2281 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
     2341 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
     2401 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
     2461 tacatgaggt tttgtataaa aatggcgggc gatatcaacg cagtgtcaga aatccgaaac
     2521 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     2581 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     2641 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     2701 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     2761 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcctgggc gaaacagcag
     2821 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     2881 ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc gcgtctttcc
     2941 tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgct
     3001 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     3061 gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt ctccttcgct
     3121 caggctcgtg cttgtgcgga agcgggcgtg ttcctgatct cgccgtttgt tggccgtatt
     3181 cttgactggt acaaagcgaa taccgataag aaagagtacg ctccggcaga agatccgggc
     3241 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aaccgtggtt
     3301 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg cgaccgtctt
     3361 accatcgcac ctacactgct gaaagagctg gctgagagcg aaggggctat cgaacgtaaa
     3421 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     3481 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     3541 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     3601 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     3661 tatcattctg tttaacgaga ctgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     3721 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcatccct
     3781 gcgctggaag aatggctgac atcggcgcta accacgccgt ttgaactgga aacccgctta
     3841 atccccgatg agcaggcgat catcgagcaa acgttgtgtg agctagtgga tgaaatgagt
     3901 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     3961 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagata
     4021 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg ttggtgtgat tcgcaaacag
     4081 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     4141 aaggacgctg cgggtaacgt tgtggtacac ggtatttttg ccagcgtacc gtactgcatt
     4201 cagttgctgg aagggccata cgttgaaacg gcaccagaag tggttgctgc attcagaccg
     4261 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
     4321 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
     4381 cacccatcgc cagatagatt gcactggcac cacagatcag accaatccag ccggcaaagt
     4441 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacagtca
     4501 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
     4561 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     4621 tcagacccag tttcggcatc agcagaatcg caaccaacgt cagccagaaa gaaccgtaag
     4681 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccggcaaaaa
     4741 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     4801 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     4861 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     4921 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     4981 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     5041 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     5101 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
     5161 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     5221 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     5281 tctttcatca gcagcggcag cagctcttcc agctcattca ccctcgcatc gaccgcgtgc
     5341 agaaactcct gtttatgttc ctcgtccata ttcttccagg tgttacgcag aaattgttcc
     5401 agtaactgtt gctcaatctc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     5461 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     5521 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     5581 tgctcgggat gcccttccat agatttaaac agttcgttgc gcatcagtac gctggagagg
     5641 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     5701 ctgcaatgct ggagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     5761 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     5821 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
     5881 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     5941 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccgcttaact gtaaatcgcc
     6001 atggtcggca gagagttgta tgcgtttaac aattcggcaa acaggaagtt tcagcgccag
     6061 atcgttggtt tcgttacgtg gcatcgcaat gacgccgagg attttatggt cgtttgcctg
     6121 cgccgtgcag cacagcatca gactaatcgc caggctggcg gaaatcgtaa aaacggattt
     6181 cataaggatt ctcttagtgg ggagaggtag ggggatggat acccactagt ttactgctga
     6241 taaagagaag attcaggtgc gtaatgtttt ctttttatta caattttttg atgaatgtct
     6301 tggctgcgat tcattcttta tatgaataaa attgctgtca gtttcacgtc ttgtcctgct
     6361 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     6421 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg
     6481 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     6541 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     6601 ttgtgtagcg attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg
     6661 caccacgcct tctattattg cctataccca ggatggtgaa actctggttg gtcagccggc
     6721 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     6781 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     6841 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     6901 gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
     6961 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     7021 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     7081 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
     7141 cctggggggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     7201 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
     7261 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     7321 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     7381 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
     7441 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     7501 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     7561 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     7621 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     7681 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     7741 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     7801 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     7861 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     7921 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     7981 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     8041 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     8101 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     8161 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     8221 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     8281 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     8341 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     8401 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     8461 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     8521 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     8581 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     8641 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     8701 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     8761 acgatcagta tggtcatgct gcgtttgagc aaggtggtat gggcggcggc ggttttggcg
     8821 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     8881 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     8941 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     9001 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     9061 gttctggtca ggtgcagatg cgtcagggat tcttcgctgt acagcagacc tgtccacact
     9121 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     9181 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     9241 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     9301 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctctattgcg
     9361 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     9421 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc
     9481 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     9541 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     9601 aaagcttcgg tggcccaacc ggtgagcaca acagcccgcg ctcaaagagc ttctttgatg
     9661 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     9721 aggcttgggt aaaattaggg tgcgttgaag atatgcgagc acctgaaaag tggcggggat
     9781 cactccccgc cgttgcactt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     9841 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     9901 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     9961 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc
    10021 gggttgatag taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
    10081 gacagatagc ctcaagt
//
LOCUS       DAZFDP010000163        10092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFDP010000163 DAZFDP010000000
VERSION     DAZFDP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..742
                     /locus_tag="ACHJ1G_004215"
     CDS             134..742
                     /locus_tag="ACHJ1G_004215"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4765969.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            840..2231
                     /gene="selA"
                     /locus_tag="ACHJ1G_004216"
     CDS             840..2231
                     /gene="selA"
                     /locus_tag="ACHJ1G_004216"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4765970.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2228..4072
                     /gene="selB"
                     /locus_tag="ACHJ1G_004217"
     CDS             2228..4072
                     /gene="selB"
                     /locus_tag="ACHJ1G_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4765971.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4262..5413
                     /gene="yiaY"
                     /locus_tag="ACHJ1G_004218"
     CDS             4262..5413
                     /gene="yiaY"
                     /locus_tag="ACHJ1G_004218"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4765972.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5578..7116
                     /gene="aldB"
                     /locus_tag="ACHJ1G_004219"
     CDS             5578..7116
                     /gene="aldB"
                     /locus_tag="ACHJ1G_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4765973.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(7335..7538)
                     /locus_tag="ACHJ1G_004220"
     CDS             complement(7335..7538)
                     /locus_tag="ACHJ1G_004220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4765974.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            7661..7984
                     /locus_tag="ACHJ1G_004221"
     CDS             7661..7984
                     /locus_tag="ACHJ1G_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4765975.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            7990..9126
                     /locus_tag="ACHJ1G_004222"
     CDS             7990..9126
                     /locus_tag="ACHJ1G_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4765976.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(9123..>10092)
                     /locus_tag="ACHJ1G_004223"
     CDS             complement(9123..>10092)
                     /locus_tag="ACHJ1G_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4765977.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2417 a         2446 c         2759 g         2470 t
ORIGIN      
        1 aacaattagg aaaatattta tatcaccatg ataaatatgt gatttatttg gaagtaacaa
       61 accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt tttcctcgca
      121 caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac gcaaactttc
      181 tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc cctataacgc
      241 ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg tgaccgaaga
      301 gggcgaatgt tggtttgatt cgccgatcat cgctgaatat attgaattaa tgaatgtcgc
      361 tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa ttgaggcact
      421 ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc gtccagcggc
      481 gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca gtctggatgt
      541 gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc tggcgactat
      601 cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc gttgcgccag gctggtgtgt
      661 cgatcgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa gttttgctcg
      721 caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc acgttacaat
      781 ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc aggtttccta
      841 tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc ttattgcgcg
      901 atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg gaattgttgc
      961 gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg cctgcgtggt
     1021 gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaagcgcag agcgcgctgc
     1081 gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga gctttacagg
     1141 cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc ctcgagtacg
     1201 atctggacga cgctggacgc ggacatcgcg atcgtgcgct ggcgcagttg ctgtgccgca
     1261 ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg ttattgatgt
     1321 tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaactg gtggagattg
     1381 gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta cacgaagtag
     1441 ggaccaccaa ccgcacgcac gcgaatgatt attgtcaggc ggtgaatgaa aataccgcac
     1501 tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa gcgatagatg
     1561 aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact gatttaggca
     1621 gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg ccgcaggagt
     1681 tgattgcggc gggcgtcagt ctggtgagtt tctccggcga caagttgtta ggcgggccgc
     1741 aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc cacccgctga
     1801 agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg ttgcgtcttt
     1861 atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt acccgcagcg
     1921 cagaggtcat tcaaatccag gcacaacgtt tacaggcccc ccttgccgca cattacggcg
     1981 cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt tcgctgccgg
     2041 ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc ggtagccacc
     2101 ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt cgtatttatg
     2161 acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt ttggagatgt
     2221 tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa ccttattgca
     2281 ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg gcatgaccat
     2341 cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctggtt ttatcgacgt
     2401 tcccggtcat gaaaaatttc tttccaacat gctggcgggc gttggtggta tcgatcacgc
     2461 gctgttggtg gtggcgtgcg atgacggtgt gatggcacag acccgtgagc atctggcgat
     2521 tttgcagctg accggtaacc cgatgctgac agtggtgctg accaaagccg atcgcgtgga
     2581 cgaagcgcgt gttgatgagg ttgaacgtca ggtaaaggag gtgctgcggg aatacggttt
     2641 tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cgaggaattg atgccctgcg
     2701 cgagcatctg cttcagttac cagaacgtga acacgccagc caacatagtt tccgcctcgc
     2761 gatcgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta cggcgttaag
     2821 cggggaagtg aaggtaggcg attcactatg gctaactggt gtaaataaac cgatgcgtgt
     2881 acgtgcgctg catgcacaaa accagccaac agaaaccgcc catgccgggc agcgtatcgc
     2941 gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact ggctgcttgc
     3001 cgatgcgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc atacgccgct
     3061 gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg gacgcgtttc
     3121 gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc tggcggataa
     3181 cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg cgcgtgtggt
     3241 gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat ggctggcgtc
     3301 tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac gcggcgcggt
     3361 taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc gcgaattgct
     3421 gcaacagcct ggttatattc aggctggtta tagcttgttg aatgcgccgg ttgccgcccg
     3481 ctggcagcgg aaaattctcg acacattagc gacttatcat gagcaacatc gcgatgaacc
     3541 tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg aagcgctggt
     3601 actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc acggttggct
     3661 gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct ggcaaaaagc
     3721 agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag agacgggtac
     3781 cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa taattaccgc
     3841 gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca atatgatccg
     3901 cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc gcttaggcgt
     3961 aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct ttacgcgtcg
     4021 tcgtggaaat gatcatttat tgcgcgacgc attattattt ccggaaaaat aagaaaatga
     4081 ttaattaatt tttaaaataa attaatacaa atttcttatg attttaaaaa aagcacattg
     4141 tttaatgaat gcaatgtgct ttttattaga ttaattttga taattaataa taatgatcat
     4201 gttttcgaat gaaaatcctc agtaagctgc ccaccctttt ttacactttc aggagtgtgt
     4261 tatggcatct tcaactttct ttattccttc tgtgaatgtc atcggcgctg attcattgac
     4321 tgatgcaatg aatatgatgg cagattatgg atttacccgt accttaattg tcactgacag
     4381 tatgttaacg aaattaggta tggcgggtga tgtgcaaaaa gcactggaag aacgcaatat
     4441 ttttagcgtt atttatgatg gcacccaacc taaccccacc acggaaaacg tcgccgcagg
     4501 tttgaaatta cttaaagaaa ataattgcga tagcgtgatc tccttaggcg gtggttctcc
     4561 acacgactgc gcaaaaggta ttgcgctggt ggcagccaat ggcggcgata ttcgcgatta
     4621 cgaaggcgtt gaccgctctg caaaaccgca gctgccgatg atcgccatca ataccacggc
     4681 gggtacggcc tctgaaatga cccgtttctg catcatcact gacgaagcgc gtcatatcaa
     4741 aatggcgatt gttgataaac atgtcactcc gctgctttct gtcaatgact cctctctgat
     4801 gattggtatg ccgaagtcac tgaccgccgc aacgggtatg gatgccttaa cgcacgctat
     4861 cgaagcgtat gtttctattg ccgccacgcc gatcactgac gcttgtgcac tgaaagccgt
     4921 gaccatgatt gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg
     4981 tgaagcaatg gcttatgccc agttcctcgc cggtatggcg ttcaataatg cttctctggg
     5041 ttatgttcat gcgatggcgc accagctggg cggtttctac aacctgccac acggtgtatg
     5101 taacgccgtt ttgctgccgc atgttcaggt attcaacagc aaagtcgccg ccgcacgtct
     5161 gcgtgactgt gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgatg cggaaggtgc
     5221 tgaagcctgc attaacgcca tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct
     5281 acgcgacctg aacgtgaaag aagaagattt cgcggttctg gcgactaatg ccctgaaaga
     5341 tgcctgtggt tttactaacc cgatccaggc aactcacgaa gaaattgtgg cgatttatcg
     5401 cgcagcgatg taatcatcat ttccacaacg gctggcaaat tgtcagccgc tttttcaact
     5461 atctctgtaa cccttgcccg taaattcgtg atagctgtcg taaagctgtt accgactggc
     5521 gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga gacgatcatg
     5581 accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc cctcaagtta
     5641 aaagcccgct atgacaactt tattggcggc gaatgggtag cccctgccga cggcgagtat
     5701 taccagaatc tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa
     5761 cgagacatcg atctggcgct ggatgctgcg cacaaagtga aagataaatg ggcgcacacc
     5821 tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga acaaaacctt
     5881 gagctgttag cgacggctga aacctgggat aacggcaaac ccattcgcga aaccagtgct
     5941 gccgacgtgc cgctggcgat tgaccatttt cgctatttcg cctcgtgtat tcgggcgcag
     6001 gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca tgaaccgtta
     6061 ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg
     6121 gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct taccccgctt
     6181 tctgtactgc tgctaatgga aatcgtcggt gatttactgc cgccgggcgt ggtgaacgtg
     6241 gtcaacggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg catcgccaaa
     6301 gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc cacgcaaaac
     6361 attattccgg tgacgctgga gctgggcggc aaatcgccaa atatcttctt tgctgatgtg
     6421 atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact gtttgccttt
     6481 aaccagggtg aagtttgcac ctgtccgagt cgtgccttag tgcaggagtc aatctacgaa
     6541 cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa cccactcgac
     6601 agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat cctcaactac
     6661 attgatatcg gtaaaaaaga gggcgcagac gtgctcaccg gcgggcggcg caagctgctg
     6721 gaaggtgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg tcagaacaat
     6781 atgcgggtgt tccaggagga gatttttggc ccggtgctgg cggtgaccac cttcaaaacg
     6841 atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg
     6901 agccgcaacg gtaatctggc ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg
     6961 accaactgct atcacgctta cccggcacat gctgcgtttg gtggctacaa acaatcaggt
     7021 atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa gtgcctgctg
     7081 gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt cgcattgggt
     7141 attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg
     7201 ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt acaatttacg
     7261 ttttaaacct acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt tctggggcgt
     7321 gttttgccag gagtttactc tcgcgtttcg atggtgagcg ggcaaaccag gccgtaaatc
     7381 accattggcg ttccgaggcc aatcagcaat aaacggtaga taattgaagg tttaatacgc
     7441 tttatctgta ctgtggatgt tttactggag ttattcatta gcttggtcaa tagttccatt
     7501 atttcaatat gataaacctc gacctgttga aaagccacgc agcacacttc agaaagtata
     7561 taaaaaacaa tatgccgtta tataaataag gaaattgcac tgcatcatgg gatttcataa
     7621 tatgcgttaa ttgttctttt ttatattctc tgagggaaat atgtttctgg attattttgc
     7681 actgggagtg cttatttttg tatttctggt gatattctat gggattatta ttttacatga
     7741 tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca ttcatgttgc
     7801 tggttgggtg agtcttttta cgctgcatgt tatctggcca tttttgtgga tttgggccac
     7861 gctttaccgc ccggagcggg gatggggaat gcaaaaccat gactcctccg ttgtgcaact
     7921 acaacagcgt attgccgggc tggagaaaca gctcgccgac atcaaatcct cttctgccga
     7981 gtaatactta tggatttact gattattttg acctatgtgg cttttgcatg ggcaatgttt
     8041 aagatcttca aaattcctgt aaataaatgg accattccca cagcggccct gggtggcata
     8101 tttattgtca gtggtttaat tctgttaatg aactataacc atccgtatac cttcaaagcg
     8161 caaaaagcgg ttatttctat tcctgttgtc ccacaggtga caggcgtggt gatcgaagtg
     8221 acggataaga aaaatacgct gattaaaaaa ggtgaggtgc tatttcgact ggacccgacg
     8281 cgttatcagg cgcgggtgga tcggctgatg gcggatatcg ttaccgcaga acataaacag
     8341 cgggcgttgg gcgcagagtt agatgagatg gcggcgaata ctcagcaggc aaaggccacg
     8401 cgggataaat tcgctaaaga gtataagcgt tacgcacgtg gcagccaggc gaaagtaaac
     8461 ccgttttcag aacgcgatat cgatgtggcg cggcaaaatt atctggcgca ggaagcctcc
     8521 gtgaagtcat cggcggcgga acaaaaacag atccagagcc agctggatag cctggtgttg
     8581 ggtgaacatt ctcaaatcgc cagcctgaaa gcacagctcg cggaagcaaa atataacctt
     8641 gagcagacga tagtgcgtgc gccgagcgat ggttatgtga cccaggtact gattcgtccg
     8701 ggtacctatg ccgcgtcgct gccgctacgt ccggtgatgg tgtttatacc cgatcagaaa
     8761 cgacaaatcg tggcgcagtt ccgtcagaac tccttgctgc gactggctcc tggcgacgat
     8821 gcggaagtgg tgtttaatgc tctgccaggt aaggtattca gcggtaagct ggcagccatt
     8881 agtccagccg ttcccggcgg agcttatcag tcgaccggca ccttacagac gttaaacaca
     8941 gcgccgggtt cagatggcgt tatcgcgacc attgaactgg atgagcacac tgatttgagc
     9001 gctttaccag acggtattta cgcccaggtg gcggtttact ctgatcattt cagccatgtc
     9061 tcggtgatgc gcaaagtgct attacgcatg accagctggg tgcattacct ttatctcgat
     9121 cattaatctt cgctttccgc cgcttgttca ggccgcagtt gctgcgactg agcaagctgg
     9181 tggcgaattt cacgcagcat ttcatccagc acatactgga cccgccaggg ctggtattcc
     9241 cgtttacgga agatagccac cagatccagc caccattcat agggttgctg aaagcagggc
     9301 accagcgtgc cgtcttgcag gtcgctctgc acacttttgt ctggcgcaaa cacaatcccc
     9361 aggtgatttc gtgccagctc cagcgcagat tgcgtgttgt cgcaaacgta atttcccttc
     9421 acgcgataat cccgcacctc tttactcccg gcgacgttga agcgccagat attcgcgtcg
     9481 tcgatcatca tcgagtcgat caaaatacag gagtgatgtt cgagttcgtc aggacggcta
     9541 atgggatgtt tctcaagata gcgctggctg gcataagcgg ttacggcata ttgggtaata
     9601 aaactggcaa ccagcgattc atcttttggt ggagcgtagc tgattaaaac atcacaatca
     9661 tcaggaaatt cgacgccttc ataaaaggca ttacgctcaa gattgcaggt ttttagcgat
     9721 aaggtgatat caccgatatc tttaatttta tcgattacat gtttggataa ataggtaatt
     9781 atgccggttg gggcgtagat ggtgacccga ccacgtttct catgcttata atctgcaata
     9841 aaattattaa gttgctcgtt tctgtccagc atgtcgttga tgtacggtag cagcgcggtg
     9901 ccaaaaggtg tgagcatcag ctgccgggtg gttcggtcaa aaacttttaa acccactttt
     9961 gattcaaaat cagcaagata tttgctgacg ttggcctgtg cgatgccaag tacagtcgcg
    10021 gcatggctga tattttcact ggcagcgatt accgagataa tttttaactc ccggtactta
    10081 agttgtaatt tt
//
LOCUS       DAZFDP010000164        10054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFDP010000164 DAZFDP010000000
VERSION     DAZFDP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1277
                     /gene="glaH"
                     /locus_tag="ACHJ1G_004224"
     CDS             300..1277
                     /gene="glaH"
                     /locus_tag="ACHJ1G_004224"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4765978.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1297..2565
                     /gene="lhgO"
                     /locus_tag="ACHJ1G_004225"
     CDS             1297..2565
                     /gene="lhgO"
                     /locus_tag="ACHJ1G_004225"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4765979.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2588..4036
                     /gene="gabD"
                     /locus_tag="ACHJ1G_004226"
     CDS             2588..4036
                     /gene="gabD"
                     /locus_tag="ACHJ1G_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4765980.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4050..5330
                     /gene="gabT"
                     /locus_tag="ACHJ1G_004227"
     CDS             4050..5330
                     /gene="gabT"
                     /locus_tag="ACHJ1G_004227"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4765981.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5641..7041
                     /gene="gabP"
                     /locus_tag="ACHJ1G_004228"
     CDS             5641..7041
                     /gene="gabP"
                     /locus_tag="ACHJ1G_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4765982.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7062..7724
                     /gene="csiR"
                     /locus_tag="ACHJ1G_004229"
     CDS             7062..7724
                     /gene="csiR"
                     /locus_tag="ACHJ1G_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4765983.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7725..8174)
                     /gene="kbp"
                     /locus_tag="ACHJ1G_004230"
     CDS             complement(7725..8174)
                     /gene="kbp"
                     /locus_tag="ACHJ1G_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4765984.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8258..8416)
                     /gene="yqaE"
                     /locus_tag="ACHJ1G_004231"
     CDS             complement(8258..8416)
                     /gene="yqaE"
                     /locus_tag="ACHJ1G_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4765985.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8599..8898
                     /gene="ygaV"
                     /locus_tag="ACHJ1G_004232"
     CDS             8599..8898
                     /gene="ygaV"
                     /locus_tag="ACHJ1G_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4765986.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8908..9432
                     /gene="ygaP"
                     /locus_tag="ACHJ1G_004233"
     CDS             8908..9432
                     /gene="ygaP"
                     /locus_tag="ACHJ1G_004233"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4765987.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9479..9883)
                     /gene="stpA"
                     /locus_tag="ACHJ1G_004234"
     CDS             complement(9479..9883)
                     /gene="stpA"
                     /locus_tag="ACHJ1G_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4765988.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2321 a         2576 c         2722 g         2435 t
ORIGIN      
        1 taatatgttt agtttactaa cggtcatttt gcagtgaagc catttactgt tttttatcga
       61 ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt tattcatttc
      121 ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgc ttttgatcac
      181 aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat
      241 tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga
      301 tgaatgcact gaccgccgta cacaataacg ctgtcgattc aggccaggac tatagcggat
      361 tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga
      421 cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat
      481 cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac
      541 cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtgg
      601 gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc
      661 tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga
      721 aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca
      781 acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgagc
      841 aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa catctggacc
      901 actatttccg ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg ccgagcaaaa
      961 acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt cgcccggtga
     1021 tgcgctatat cgaccagttc gtccagccaa aagattttga agaaggcgtg tggttgagcg
     1081 agctttccga tgccattgaa accagcaaag gcattctttc tgtacccgtt cccgttggca
     1141 aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt actccgcacc
     1201 cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc actcaccact
     1261 accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt gattattggc
     1321 ggcggcatca tcggcatgtc gaccgccatg caactgattg atgtttatcc ggatgcccgc
     1381 attgcgttgc tggaaaaaga gtccggcccg gcctgtcacc agacgggcca caacagcggc
     1441 gtgatccatg ccggggtcta ttacacgccc ggcagcctga aggcgcagtt ttgcctggcg
     1501 ggaaaccgcg ccaccaaagc cttttgcgat caaaacgaca ttcgctatga caactgcggc
     1561 aagatgctgg tcgccacgtc cgatctcgaa atggaacgga tgcgcgcgtt gtgggaacgc
     1621 acggcggcga acggtatcga gcgcgagtgg ttaaacgccg aggaactgcg cgagcgcgaa
     1681 ccgaatatca ccgggctcgg cggcattttt gtgccgtcca gcggcattgt cagctatcgc
     1741 gaagtcacag cggcaatggc aaaaatcttc caggccagag gcggtgagat tatttataac
     1801 gccgaagtca gcgccctcag tgagcataaa aacggcgtgg tcgtccgaac ccgtcagggc
     1861 ggcgaatatg aagcatcgac gctgattagc tgttccgggc tgatggctga ccggctggtg
     1921 aaaatgctcg gcctcgaacc gggttttatt atctgcccgt tccgtggcga gtatttccgc
     1981 cttgcgccgg agcataacca gattgttaac catctgattt accccattcc cgacccggca
     2041 atgccatttt tgggcgtgca tctcacccga atgatcgatg gcagcgtcac cgtcgggcca
     2101 aacgcggtgc tggctttcaa acgcgaaggc tatcgcaagc gcgacttctc atttagcgac
     2161 acgctggaga ttttgggctc gtcggggatt cgccgggtgc tgcaaaacca tctacgctca
     2221 ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcgcct ggtgcaaaag
     2281 tattgtcccc ggctttcgtt aagcgatctc cagccctggc ccgccggtgt gcgggcgcag
     2341 gcagtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac cccacgcacg
     2401 atccacacct gcaatgcgcc ctccccggca gcgacatcag caattcctat tggtgcgcat
     2461 attgtcagca aggtacaaac gctgttggca agccagagta accccggacg cacgctgcga
     2521 gcggcacgta gtgtggatgc cttacacgcc gcatttaatc aataaccttt gaaaacagga
     2581 cgtaacaatg aaacttaacg acagtaactt attccgccag caggcgttga ttaacgggga
     2641 atggctggac gccaacaatg gtgaagtcat cgacgtcacc aatccggcga acggcgacaa
     2701 gctgggtagc gtgccgaaaa tgggcgctga tgaaacccgc gccgctatcg acgccgccaa
     2761 ccgcgccctg cccgcctggc gtgcgctcac cgccaaagaa cgcgccaaca ttctgcgcaa
     2821 ctggttcaat ttgatgatgg agcatcagga cgatttagcg cgcctgatga ccctcgaaca
     2881 gggcaaacca ctggccgaag cgaaaggtga aatcagctac gccgcctctt ttattgagtg
     2941 gtttgccgaa gaaggcaaac gcatttatgg cgacaccatt cccggtcatc aggccgataa
     3001 acgcctgatt gttatcaagc agccgattgg cgttaccgca gctatcacgc cgtggaactt
     3061 cccggcggcg atgattaccc gtaaagccgg tccggcgctg gcggcaggct gcaccatggt
     3121 gctgaagccc gccagtcaga caccgttctc tgcgctggcg ctggcggagc tggcgattcg
     3181 cgcgggcatt ccggctggcg tgtttaacgt ggtcaccggt tcggcaggcg cggtcggtaa
     3241 tgaactgacc agcaacccga tggtacgcaa actgtcgttt accggttcga ccgaaattgg
     3301 ccgccagtta atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg
     3361 caacgcgccg tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg aaggcgcgct
     3421 ggcctcgaaa ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca
     3481 ggacggcgtg tatgaccgct ttgccgaaaa attgcagcag gcggtgagca aactgcacat
     3541 cggcgacggg ctggataaag gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc
     3601 aaaagtggaa gagcatattg ccgatgcgct ggagaaaggt gcgcgtgtgg tttgcggcgg
     3661 taaagcggat gaacgcggcg gcaacttctt ccagccgacc attctggtgg acgtgccggc
     3721 caacgccaaa gtgtcgaaag aagagacgtt cggccccctc gccccgctgt tccgctttaa
     3781 agatgaagcc gatgtgattg cgcaagccaa tgacaccgag tttggccttg ccgcctattt
     3841 ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt acggcatcgt
     3901 cggcatcaat accggcatta tttccaatga agtggccccg ttcggcggca tcaaagcctc
     3961 gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa tcaaatatat
     4021 gtgcatcggt ctttaactgg agaatgcgaa tgaacagcaa taaagagtta atgcagcgcc
     4081 gcagtcaggc gattccccgt ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg
     4141 aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg
     4201 cggtgctcaa taccgggcac ctgcatccga aggtggtggc cgcggtggaa gcgcagttga
     4261 aaaaactgtc gcacacctgc ttccaggtgc tggcttacga gccgtatctg gagctgtgcg
     4321 agattatgaa tcagaaggtg ccgggcgatt tcgccaagaa aacgctgctg gttacgaccg
     4381 gttccgaagc ggtggaaaac gcggtaaaaa tcgcccgcgc cgccaccaaa cgtagcggca
     4441 ccatcgcttt tagcggcgcg tatcacgggc gcacgcatta cacgctggcg ctgaccggca
     4501 aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt
     4561 atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc caccggatct
     4621 tcaaaaatga tgccgcgccg gaagatatcg ccgccatcgt gattgagccg gttcagggcg
     4681 aaggcggttt ctacgccgcg tcgccagcct ttatgcagcg tttacgcgcg ctgtgtgacg
     4741 agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcggggcgt accggcacgc
     4801 tgtttgcgat ggagcagatg ggcgttgcgc cggatctcac tacctttgcg aaatcgatcg
     4861 cgggcggctt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggat gccgtcgctc
     4921 caggcggtct gggcggcacc tatgccggta acccgattgc ctgcgtggcg gcgctggaag
     4981 tgttgaaggt gtttgagcag gaaaatctgc tgcaaaaagc caacgatctg gggcagaagc
     5041 taaaagacgg attgttggcg atagccgaaa aacacccgga gatcggcgac gtacgcgggc
     5101 tgggagcgat gattgccatc gagctgtttg aagatggcga tcacagcaaa ccggacgcaa
     5161 aactcaccgc cgagatcgtg gcacgcgccc gcgataaggg cctgattctt ctctcctgcg
     5221 gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac gctcagatcc
     5281 gtcagggtct ggagatcatc agccagtgtt ttgctgaggc gaagcagtag ctatgtccgt
     5341 ttgtaagagt taaacgttgc taagggaatg ataacgatgc agtaaaaaat aataacgatg
     5401 aggcgatcga caaagttaaa aacgactgcg gactgtcccc tcgctcctct ggggagaggg
     5461 ttagggtgag gggtaatcac gcggctacac cctcaccccg gccctctccc taagggagag
     5521 ggagaaaact ctcccgcttt cgtttttaag attttgttga ccgtctcaat taaacgcgtg
     5581 acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg
     5641 atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc
     5701 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
     5761 gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact ggtggttatg
     5821 attatgcgga tgttggcgga aatggcagtt gccacgcccg ataccggttc gttttccacc
     5881 tatgccgata aagccattgg ccgctgggcg ggttatacca tcggctggct gtactggtgg
     5941 ttttgggtac tggttatccc gctggaagcc aacatcgccg ctatgatcct gcactcgtgg
     6001 gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt
     6061 aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
     6121 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat
     6181 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggcttc
     6241 ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
     6301 accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc taccaactcg
     6361 gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtagt ggcgttaata
     6421 ccgtggaata tgccggggct gaaagccgtt ggttcttatc gctcggttct ggaattgctc
     6481 aatattcccc atgcgaaatt aatcatggac tgcgtgatat tactttccgt aaccagctgt
     6541 ctgaactcgg cgctgtatac cgcgtcaagg atgctctact ccttaagccg tcgcggtgat
     6601 gcgcccgcgc taatgggcaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc
     6661 tccaccggag cggcattttt aacggtggtg gtgaactatt acgcacctgc gaaagtgttt
     6721 aaattcctga tagacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt
     6781 tcacagttgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgcatg
     6841 tggctttatc cctggctcac ctggctggtc atcggcttta ttacctttgt gttggtagtg
     6901 atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg
     6961 attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca
     7021 cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg
     7081 atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat
     7141 tacggatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagcac
     7201 tttctcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg
     7261 cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga
     7321 tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa
     7381 aagcgcatct gctcagtaag ctggaagcct gtgacgccag cgagaaaatg cttgatgagt
     7441 gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt tctcactatt
     7501 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt atctggctgc
     7561 ggcgaacggt gctttcggta gaaatgctgg aggataaaca cgatcagcac cagaccctga
     7621 ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc cagcatttac
     7681 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata
     7741 cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg
     7801 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
     7861 tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga
     7921 tcatcgacac tggcaatacc ggaaatattc ccaaccgcaa caaggatttt ctccttcgcc
     7981 tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact
     8041 ttatcggcat caggtatacc ggttttgctc agatgctcct gcaccttctt cgcctgatcg
     8101 tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa
     8161 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
     8221 aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga
     8281 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
     8341 cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg gtgatgacga
     8401 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
     8461 ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcttac gcttttactc
     8521 tcatttacgc tgctgaggct ggtatatgtt gcatttttag tttagagttt acttaattta
     8581 gaaaatactt aaataaatat gactgaactc gcgcaattac aggccagtgc cgaacaggca
     8641 gcggccttat tgaaagcaat gagccatcct aaacgattgc tgattctgtg catgcttagc
     8701 ggttcccccg gcaccagcgc gggagagctg acgcgcatta ccggactgag tgcctctgcg
     8761 acatcacagc atctcgctcg tatgcgggac gaagggctta tcgacagcca acgggatgcc
     8821 caacgcattc tatattccat taaaaatgag gcggtaaatg ccattatcgc caccctgaaa
     8881 aatgtctatt gtccgtaagg agtcactatg gctttgacaa ccatttcgcc gcatgatgca
     8941 caagaattaa tcgcacgcgg cgcaaagtta atcgatattc gtgacgctga tgaatatctt
     9001 cgtgaacata ttcctgaagc agatctggct ccattatccg tgctggaaca gtcaggtctt
     9061 ccacctaaat tacgtcgcga gcaaattata ttccactgcc aggcaggtaa acgcaccagt
     9121 aataacgccg ataaattagc agcgattgcc gcccccgcag aaatattttt actcgaagat
     9181 gggattgatg gctggaaaag agctggattg ccagtagcgg taaataaatc tcaacccttg
     9241 ccgttaatgc gccaggtgca gatcgctgcg ggtggtttaa tattaatcgg cgttgtactg
     9301 ggttataccg taaatagcgg tttcttctta ttaagtggct ttgttggtgc cgggttactg
     9361 tttgcaggaa tcagcggttt ttgtggaatg gcaaggttgt tagataagat gccgtggaac
     9421 caacgaactt gagaagcgac gccggacgcg ccctagcagc gacatccggc ctcagtaatt
     9481 agatcaggaa atcgtcgaga gatttacctt ctgccagcgc ctgagcaatt ggcttcggtg
     9541 tacggccctg accggtccag gttttagttt caccgttaac atcggtgaat ttatatttcg
     9601 ccggacgcgg ctggcgtttt ttaccagcgc gtggtgcagc agcagagcta ttacctaata
     9661 actcttccgg gttaattccg tcagccttca tcagctccag ccaggtgcta attttttcct
     9721 gacgctcagc cagttcacgc tgctgctgtt cttcttcttc acgtctttct ttagtgacaa
     9781 ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga gccatcgcac
     9841 ggagagtgcg aatgttattt aaactttgta acattacgga cataataaag aaaacctttt
     9901 aacgccaaaa caaaaaagta tttcgtcgcg tagtgtaagc gatcacttat tatatttcca
     9961 caaaatttaa tctatttaca gtcctgcgcg agattatttc agctgttatt ttctgaaact
    10021 ctcagaataa tttcaagatt atatattagc catt
//
LOCUS       DAZFDP010000165         9909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFDP010000165 DAZFDP010000000
VERSION     DAZFDP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..616)
                     /locus_tag="ACHJ1G_004235"
     CDS             complement(11..616)
                     /locus_tag="ACHJ1G_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO4765989.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLIINMLMCAGHY"
     gene            complement(613..2301)
                     /gene="yfaA"
                     /locus_tag="ACHJ1G_004236"
     CDS             complement(613..2301)
                     /gene="yfaA"
                     /locus_tag="ACHJ1G_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4765990.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2450..5077)
                     /gene="gyrA"
                     /locus_tag="ACHJ1G_004237"
     CDS             complement(2450..5077)
                     /gene="gyrA"
                     /locus_tag="ACHJ1G_004237"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4765991.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5224..5946
                     /gene="ubiG"
                     /locus_tag="ACHJ1G_004238"
     CDS             5224..5946
                     /gene="ubiG"
                     /locus_tag="ACHJ1G_004238"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4765992.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6074..9808)
                     /gene="yfaL"
                     /locus_tag="ACHJ1G_004239"
     CDS             complement(6074..9808)
                     /gene="yfaL"
                     /locus_tag="ACHJ1G_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4765993.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEDYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2316 a         2743 c         2385 g         2465 t
ORIGIN      
        1 tttattttga ttaataatga ccagcgcaca ttaacatatt gataattaat gaaattttaa
       61 tatacaactt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
      121 tcgtggcgct tccggtgtgg tagatgacgt aacgccccat ccagaccatt aagtgctggg
      181 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcctggccaa
      241 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg gttttcccgt
      301 tcccctgatt ccagttttgc gccagttgac gctgtccagg tgttagcgtc atctctggcg
      361 gcaaatactg gctagagaaa ccgttacttt ttagccattt actatcgtga actttcagcg
      421 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
      481 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt
      541 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
      601 gcagcccgtg cctcatagcg gctgccaggt tatgggtagc cactgccagg cggcaccggg
      661 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
      721 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
      781 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
      841 gccatcagtg ggtattacat ccaccattgc cgggcggttt ttattcagtg tttgcagggc
      901 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
      961 cacgcggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     1021 ctgaccgtat cgggaactca cttcgcgacg ccagatttgc gcttcgccct gctgagtctg
     1081 gcttatcggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     1141 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     1201 ggtttgcaat tttgagtctg cataccagca cagccccgcg gcaccgtcta acgccccatt
     1261 caacttgtcg ttttcctgac tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     1321 cggcaccacc acacagaagc tggccccggc aggcatactg ttccagaccg gcgtaaaatc
     1381 gaaactggta tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     1441 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     1501 cagcaacctg gcgctgacca cgatgcgctg gcgtactggc gttttttcag cagtacgctc
     1561 ttccaggcca aagcttgctt gccagcgttt tttgccgctc aacaaatcac ctgcgatcgc
     1621 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtgctgg aaaacaccag
     1681 catcttgtcc tgataagtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     1741 aacaggaaca gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     1801 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     1861 cagcaccata taatgtgaaa gatggccttg tttatcgtgc cacagcgctg cctgcgcggg
     1921 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     1981 acgacgaatg ctgccttcaa tgcccagacg atcggcatga ttctgataat agaaaacgaa
     2041 atcttcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     2101 cagagagtcg ctgtcgagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     2161 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     2221 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     2281 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     2341 cacagccaaa ctttaccgtg ccctaatacg acaaaagccc agactttgca gcctggactt
     2401 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     2461 ctggctcgtc gtcaacgtcc acttccggag cgatttcatc gtccccttcc gcggcactgc
     2521 catcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgttgc agacccacta
     2581 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cctacgatgc
     2641 tgatttccga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     2701 cgcagtcatc tacctgtacc gcgccaacca ctaaaccatt acgttcagta accttaatgg
     2761 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgcg gtacgtttac
     2821 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     2881 cgactttatc gccttcacct aagcgaatac cgcgaacacc ggtggtgttg cagcccatcg
     2941 cacggacaga agactcttta aagcgcacca ctttaccttc agcggagaac agcattactt
     3001 cgtcttcgcc gctggtcagg tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     3061 ccactttacc ggcggtacgc agacggttga actcggtgag gacagttttc ttcacggtac
     3121 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcact ggcaggatcg
     3181 cagtgatacg ttcgtcctgc tccagcggca gcaggttgac gatcggacga ccgcgcgcgc
     3241 cacgagtggc ttccggcaac tgataaactt tcatcgaata gacgcgacca cggctggaga
     3301 agcacagaat atggtcgtga gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     3361 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     3421 gctgatactt aacgtagccc tggtgagaga gcgtcactac cacatcttcc tgagtgatca
     3481 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     3541 actgttcacg aaccagctcc agctcttcac ggatcacttc catcaaacga tcggcgctac
     3601 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     3661 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     3721 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     3781 gcgcagcatc gtcgccagca cgttcgagca tcgcggcaac gttgcccagc tgccacggat
     3841 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     3901 tcgggtcgat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     3961 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     4021 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     4081 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     4141 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     4201 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     4261 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgaccggttt
     4321 tggcgtcaac ttccacttct gcgcgagcgc ggatatacac cttgccgcga ccggtacggt
     4381 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     4441 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     4501 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     4561 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     4621 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     4681 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     4741 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     4801 ccatacggac gatcgtgtca taaaccgccg agtcaccatg gggatggtat ttaccgatta
     4861 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     4921 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     4981 cacggccaac aatgaccgac atcgcataat ccagatagga gctcttcagc tcttcctcaa
     5041 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     5101 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     5161 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     5221 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accacgaaga gatcgctaaa
     5281 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     5341 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     5401 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     5461 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaaact gcacgcgctg
     5521 gaaagcggca ttcaggtgga ttatgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     5581 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtccc cgatccgcag
     5641 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     5701 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     5761 atggtgccca aaggcacgca tgatgtgaag aagtttatta aaccggcaga actgcttggc
     5821 tgggtagatc agacaagcct taaagagcga catataaccg ggctacatta caacccgctc
     5881 actaatactt ttaaactcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     5941 aagtgaggtt gttgtttggc cgagccaatg cctgatgcga cgcttgccgc gtcttatcag
     6001 gcctacaaat gctccccgta ggccggataa ggcgtttacg ccgcatccgg caaccgtgcc
     6061 gactagacag tgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     6121 catcactccc tttctgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc
     6181 ccgtgactcc cacttttatt tcaccccgtc gcttcaccgc atcgtcactg atagtgctgc
     6241 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     6301 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     6361 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     6421 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     6481 cggcttccag cgaggcgata atccccgacg agtggtaatg atctgtgcca tcgtcttgtt
     6541 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     6601 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     6661 tatcggcgcg agttccggtc atattcgagc ggctctcgcc ctgattatca ctgtagccac
     6721 caaccgcccc aagcatccac tcgccatcat cgccccagcg cccgctaaac aggtcaccgc
     6781 taagctgcac cgtagaagtg tcttcatgct gagccagttg ccctgccgcc gtgtaatgat
     6841 aacgtccgcc gataacacgt aaattcagcg tctgaccatt gccacctgca tgatcgcgtc
     6901 gctccatcat aaacgcctga tttgccgccc gcagattatt gagataaccg ccaactttgg
     6961 cattcagcac cggctggtaa gcggggattg gatcgggtgt tgggtcagga tcgggtgttg
     7021 ggtcagggtc tggatcaggt ggcaatggtg gcgttacttc ttgcgatcgc agataccagt
     7081 cgttgttatc ttccaccagc gtgtagtcat atgctcccat attgacgtag ccgctgcctg
     7141 ccagactgaa cttcgcattg ttttgaaact gcgtgggatc ggctgcgaaa tcaaccactt
     7201 taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt
     7261 tgccagcagt attaccgttc aacaccaatt gatcgcttac cgagtcatcg ccgtttaatt
     7321 cgctatcgag cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc
     7381 cagcgacgcc attttgcagg ctaaccgcac cggacgtatt ggtcagattg ccattaaccg
     7441 tcacgtcgga aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca
     7501 acgtcacgcc atcggtggca ttcaccagcg tcccatcgcc gttaaacagt gaggcattaa
     7561 gggcgacaga agtatcctgc ccggtcaggc gcaaaaccgt accatcgctg atgtcgatag
     7621 tgccgctgga agtagcatca atagattgaa tatcctgatc cgcgccagta acgaacgtcg
     7681 ccccatcacc gacccacagt gacgaagcat aaggaaggat atccgcgaca tcgcctttca
     7741 gggtgccttc ttcgacacgt accgccgact gcgttgtacc gctggcggtc agctccagcg
     7801 tacccgcccc cgttttagtc aatgtgctac cctcatcctg atgctgcccg ctgctgtcag
     7861 ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc
     7921 gcatttcaat atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa
     7981 tctccatcac gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat
     8041 ctccaagctg tgagttgtcg ctcacctcca gcataccgga ggcgatttgt gttgtgccca
     8101 aataactatt attgtttgcc agacttacct gcccgtcgcc cgcatcgacc accacgtaga
     8161 cgccatcttt cccgtcaccg ccgatatgca tatcgtttcc acctgcaaga tctaccacat
     8221 cctgactgcc gataaccgtt ccgctgctgc cgctgacttc aaggccatcg ttgtatgatg
     8281 tgccactctg ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg
     8341 actgcggatc gtcctggaga gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg
     8401 caccatcatc aacgactata tcgccggtta gcgccatcga ctgcgccccc gacagcacgt
     8461 agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc
     8521 cttgattaac agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg
     8581 tcaatgagtg cacaaaggtt tgttgggtgg agccaacgtt tagttctgcc tgattctggt
     8641 acttatcaat actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg
     8701 tatcgccgac attcatcagg gagttgctgc gacccagggt aacttcaccg ttttcaatct
     8761 gcatctcacc agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt
     8821 tggtgatcaa cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccaa
     8881 taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa
     8941 agccacccgc cgcagtggaa ggaccatctc catagcctgc tgcgctgttg ttctcatcca
     9001 ctaacacgcc gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag
     9061 ccgtcgcgct attggtatat atcgccccgc cataatcttc ggcagtgtta tttgtaaagg
     9121 cggtgttatt tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa
     9181 catcgattgc cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat
     9241 caaaaataac atcacttaaa taaacgtcat tattaatggt ataaattgcg ccacctttgc
     9301 catcattagc gatgttattg cgaaacatgg cgttagtgac acgtaaatcg acgacaccag
     9361 tatcattagt accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccagaaa
     9421 aaataacatc agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat
     9481 tatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta
     9541 atagtgaaaa ttccgctcct tgttgcaaaa atacggctcc tccgctggcg ttattggtca
     9601 tatccgaaaa aaccagccat tgcccatcgg cgacactcca gtcctgcgta atgcctgaaa
     9661 ggctttgcct gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg
     9721 ccgccgcgaa accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat
     9781 actccttgcg tagaaagata atccgcatat tcatctaacc atcattttat ataagacggc
     9841 atatttaaac gcattataca atatgggtta tctttctttt gtaaattgtc caacccgatg
     9901 caatggcta
//
LOCUS       DAZFDP010000166         9899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFDP010000166 DAZFDP010000000
VERSION     DAZFDP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..618
                     /gene="lpfA-O113"
                     /locus_tag="ACHJ1G_004240"
     CDS             46..618
                     /gene="lpfA-O113"
                     /locus_tag="ACHJ1G_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO4765994.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            666..1397
                     /gene="lpfB-O113"
                     /locus_tag="ACHJ1G_004241"
     CDS             666..1397
                     /gene="lpfB-O113"
                     /locus_tag="ACHJ1G_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO4765995.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1422..3944
                     /gene="lpfC-O113"
                     /locus_tag="ACHJ1G_004242"
     CDS             1422..3944
                     /gene="lpfC-O113"
                     /locus_tag="ACHJ1G_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO4765996.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3955..5028
                     /gene="lpfD-O113"
                     /locus_tag="ACHJ1G_004243"
     CDS             3955..5028
                     /gene="lpfD-O113"
                     /locus_tag="ACHJ1G_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO4765997.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            5276..6316
                     /gene="pstS"
                     /locus_tag="ACHJ1G_004244"
     CDS             5276..6316
                     /gene="pstS"
                     /locus_tag="ACHJ1G_004244"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4765998.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6403..7362
                     /gene="pstC"
                     /locus_tag="ACHJ1G_004245"
     CDS             6403..7362
                     /gene="pstC"
                     /locus_tag="ACHJ1G_004245"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4765999.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     IAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7362..8252
                     /gene="pstA"
                     /locus_tag="ACHJ1G_004246"
     CDS             7362..8252
                     /gene="pstA"
                     /locus_tag="ACHJ1G_004246"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4766000.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8343..9116
                     /gene="pstB"
                     /locus_tag="ACHJ1G_004247"
     CDS             8343..9116
                     /gene="pstB"
                     /locus_tag="ACHJ1G_004247"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4766001.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9131..9856
                     /gene="phoU"
                     /locus_tag="ACHJ1G_004248"
     CDS             9131..9856
                     /gene="phoU"
                     /locus_tag="ACHJ1G_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4766002.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2546 a         2265 c         2525 g         2563 t
ORIGIN      
        1 agtgatgatt catggtaaag gatatattat atcaatggag tttttatgaa gcgtaatatt
       61 ataggcggtg cattcactct ggcatctcta atgctggccg ggcatgcact ggcagaagat
      121 ggtgttgttc acttcgtcgg tgaaattgtc gacactactt gtgaagttac ctccgataca
      181 gccgatcaaa ttgtcccact gggtaaagtc agtaaaaatg cattttcagg tgtaggtagt
      241 ctggcgtcgc cacagaagtt cagtattaag cttgaaaatt gcccggcaac gtacactcaa
      301 gcagccgttc gttttgatgg tacagaagcg cctggcggcg acggcgacct gaaagtgggt
      361 acgccgctta cagcaggcaa ccctggtgat tttaccggta caggacaagc gattgcggca
      421 accggcgttg gtattcgtat ttttaaccag tccgataatt cgcaggttaa actttataac
      481 gactctgctt ataccgctat cgatgctgaa ggcaaggctg aaatgaagtt tattgcacgc
      541 tatgtggcaa ccaatgcgac cgtaacggct ggtacggcga acgctgattc acaatttact
      601 gtcgaatata agaaataatt tatcaagggc ctttttattc agttggcttt ggccagggag
      661 tcgttatgtc cagaaaaagt tgtttaatca ggctctttaa aacaacagtt tcatgtaagt
      721 tatttaccgc gattatactc agcgtttttt taggccaacc agcattaact tatgcaggcg
      781 ttgtcattgg tggtacgcgg gtggtttact taagtaataa tgctgataaa tctatttctg
      841 tatttagcaa agaagaaaaa ataccttatc tcatccaggc ttgggtagat ccatttaata
      901 aagaggataa aagcaaagcg ccgtttaccg ttattccgcc agtatcacga ttagaacctt
      961 cgcaagaaaa aatattacgc attattcaca ccaaaggtgt gtcattgcct gatgatcgcg
     1021 agtcagtctt ctggctgaat attaaaaata ttccgccatc agcgagcaac aaagcgacga
     1081 actcgctgga aatagcagta aaaacgcgta tcaagctgtt ctggcgacca gcaaatatcc
     1141 gccttattcc cgaagatgct gcgccgaaag tgaaatggcg ccgggaaggg cgaaatctga
     1201 tagcagagaa tcccaaccca attcatatca gtgtgatgga tgtcatcgtt gatggccatg
     1261 atgtgcccct caatatgatc cgtccttttg aaacgctcac gttaccttta cctgctaatt
     1321 ctgcgggagg ccaaatgacc tggcgattta ttaatgatta cggcgcagtc agtgacccga
     1381 taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg accagaatag
     1441 tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg gccgaggtca
     1501 gtgtattcaa tccggcgctt ctggaaatcg accatcaatc cggagtcgat attcgccagt
     1561 ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt atcaacggta
     1621 aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct gatctgcacg
     1681 cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt gatgcgctca
     1741 aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt attgaaggct
     1801 catcctggca gtttgacagt gataaattgc agctgaatat atccattccc caaatctaca
     1861 tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg attaacgcgc
     1921 tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat ggttcacaag
     1981 agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg tggcggctac
     2041 gtaattatag tactttaaac accagcgatg gccgtgcgga atacaactcc attagtacct
     2101 ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat acgtggacgg
     2161 cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact gataacgata
     2221 tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca aagtccaacg
     2281 ccaccgtcat cattcggcag aatggctacg tgatttatca gtctgccgtt ccacaaggtg
     2341 cttttgagat caccgatctc aacaccgcca gtacaggtgg cgatttggac gtaaccatca
     2401 aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg gcgattctta
     2461 aacgtgaagg tcagacagat gttgatgtca gcgtgggtga attgcgcgat gaagacggat
     2521 ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg atcactttat
     2581 atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc ggtaaagatc
     2641 ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat tttagccatg
     2701 atgatacaga aacgggtcag tcatatcgct ttctctattc aaaacgattt gacgacacag
     2761 acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat accctcaatg
     2821 agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac cgacgtagtc
     2881 gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta tacctgacat
     2941 taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa tttggctaca
     3001 gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat actgccagac
     3061 aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac atgctctctt
     3121 tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct gtttcgcaaa
     3181 acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg gaacgaaata
     3241 acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt ggcggtaata
     3301 tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac tacagccaaa
     3361 attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc gaaggggtaa
     3421 cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg gccgccgggt
     3481 tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg gtcaagacac
     3541 agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc tcgaagtcga
     3601 atatagaact tgataatacc gtcgttacga tggtttccac gcgtggtgca gtggttaaag
     3661 cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat gcaaatggta
     3721 aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca gattcaggaa
     3781 ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga caggttacgt
     3841 tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcactt tcgacaccag
     3901 aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat ttttatgaac
     3961 aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag tgtagcactt
     4021 gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac gcttgatttc
     4081 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
     4141 actaatggat cctcatatcc tggacgctgt cattgtgata ctggtaacgt aggagaattc
     4201 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
     4261 aatattaatt attatgatct taatccgaat cttgatgttg ggattgctat agatattctt
     4321 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
     4381 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
     4441 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
     4501 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
     4561 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttacagcca
     4621 gtttattttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
     4681 gtaacaacac ataaaattac taaaacggtc actattgagt gtgaaaacct ggggatactg
     4741 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
     4801 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
     4861 aaagtgaacg gtggtgagtt gccaacagac atgggtaaat caacagtgta tggtgaaaaa
     4921 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccatccccg
     4981 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
     5041 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     5101 gacaaaaaga tgacatgtgg gattttcatc ttcacattta aaatcagtaa gttatttggt
     5161 aattcctttc tgtcataaaa atgtcatatt ccttacatat aactgtcacc tgtttgtcct
     5221 attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa
     5281 agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga gcgctttctc
     5341 tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc
     5401 caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg
     5461 ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc
     5521 gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg
     5581 cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg
     5641 taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc
     5701 caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga
     5761 tggctccggg acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa
     5821 aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa
     5881 cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc
     5941 ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt
     6001 tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt
     6061 cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt
     6121 cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaagtgc tgaaattctt
     6181 cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc
     6241 ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg
     6301 taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat
     6361 tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagcct
     6421 gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg
     6481 gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg
     6541 ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac
     6601 gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg
     6661 ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagat tgcgcctggc
     6721 tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt
     6781 tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg
     6841 gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt
     6901 ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg
     6961 gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt
     7021 attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt
     7081 gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc
     7141 tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtatatgcc gggcaacagt
     7201 atcacctctg cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca
     7261 ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag
     7321 tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc
     7381 aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa
     7441 accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct
     7501 ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg
     7561 aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg
     7621 ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt
     7681 atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca
     7741 ttctgctctc cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac
     7801 agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga
     7861 ttgttatccg caccaccgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg
     7921 cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg
     7981 tgtccgggat catgaccggt atcttgctgg cgattgcccg tattgctggt gaaaccgcgc
     8041 cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg
     8101 ccaacctgcc ggtgacgatc tttaagtttg cgatgagtcc gtttgcggaa tggcagcaac
     8161 tggcctgggc cggggtattg atcattaccc tgtgcgtact gctgctgaac attctggcgc
     8221 gcgttgtttt tgcgaagaat aaacacggtt aacactttgc cggatgcggc gtgaacgcct
     8281 gatccggcct acggtaagcc tgattagcga agtgcatcag gcacgatgag gaaaagattg
     8341 caatgagtat ggttgaaact gccccgagta aaattcaggt tcgtaatttg aacttctact
     8401 acggcaaatt ccatgccctg aaaaacatca acctggatat cgctaaaaac caggtaacgg
     8461 cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct gcgtaccttc aacaaaatgt
     8521 ttgaactgta cccggagcag cgtgcggaag gtgaaattct gcttgatggc gacaacatcc
     8581 tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt gggcatggtg ttccagaaac
     8641 cgacgccgtt tccgatgtcc atctacgaca acatcgcttt tggcgttcgt ctgtttgaga
     8701 agctctcccg tgccgacatg gacgagcgcg tgcagtgggc attgaccaaa gccgcattgt
     8761 ggaacgaaac caaagataaa ttgcaccaga gcggttactc tctctctggt ggtcagcaac
     8821 agcgtctgtg tattgcgcgt ggtatcgcca ttcgcccgga agtgctgctg ctcgacgaac
     8881 cgtgttcggc gctcgaccct atctctaccg ggcgtattga agagctgatc accgaactga
     8941 agcaggatta caccgtggtg atcgtcaccc acaacatgca gcaggctgcg cgttgttccg
     9001 accacacggc gtttatgtac ctgggcgaat tgattgagtt cagcaacacg gacgatctgt
     9061 tcaccaagcc agcgaagaaa caaacagaag actacatcac cggtcgttac ggttgattca
     9121 ggagtgcgtt atggacagtc tcaatcttaa taaacatatt tccggccagt tcaacgccga
     9181 actggaaagt atccgcacgc aggtgatgac catgggcggc atggtggagc agcagctttc
     9241 tgatgcaatc accgcgatgc ataaccagga cagcgatctg gcgaagcgcg tgatcgaagg
     9301 cgacaagaac gtcaacatga tggaagtggc gatcgatgaa gcctgcgtgc gcattatcgc
     9361 caaacgtcag ccgacggcga gcgacctgcg actggttatg gtgatcagta aaaccattgc
     9421 cgagctggag cgtattggcg acgtggcgga caaaatctgc cgtactgcgc tggagaaatt
     9481 ctcccagcag catcagccgt tgctggtaag tctggagtcg ctgggccgtc ataccatcca
     9541 gatgctgcac gacgtgctgg acgcgttcgc gcggatggac attgacgaag cggtacgtat
     9601 ttatcgtgaa gataaaaaag tcgatcagga atacgaaggt attgttcgtc aactgatgac
     9661 ctacatgatg gaagattcgc gtaccattcc gagcgtactt actgcgctgt tctgcgcgcg
     9721 ttctatcgaa cgtattggcg accgctgcca gaatatttgt gagtttatct tctactacgt
     9781 gaaggggcag gatttccgtc atgtaggcgg tgatgagctg gataaactgc tggcgggaaa
     9841 agatagcgac aaataattca ccagacaaat cccaataact taattattgg gatttgtta
//
LOCUS       DAZFDP010000167         9738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFDP010000167 DAZFDP010000000
VERSION     DAZFDP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHJ1G_004249"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHJ1G_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4766003.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="ACHJ1G_004250"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="ACHJ1G_004250"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4766004.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="ACHJ1G_004251"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="ACHJ1G_004251"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4766005.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(4357..4491)
                     /locus_tag="ACHJ1G_004252"
     CDS             complement(4357..4491)
                     /locus_tag="ACHJ1G_004252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766006.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="ACHJ1G_004253"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="ACHJ1G_004253"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4766007.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="ACHJ1G_004254"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="ACHJ1G_004254"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4766008.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="ACHJ1G_004255"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="ACHJ1G_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4766009.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /locus_tag="ACHJ1G_004256"
     CDS             complement(8992..9609)
                     /locus_tag="ACHJ1G_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4766010.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
BASE COUNT         2140 a         2479 c         2758 g         2361 t
ORIGIN      
        1 attccactcc gcgccgctca tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca caatttatgt gggtactgac cgtaaacccg
      421 catagtttac cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaacacaat gtttactctg gtgcctgaca tttcaccgac
      601 aaagcccagg gaacttcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccg atccatcttt ccagcacata
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg gcaacccaac
      781 gcgcgatgtg gttcagcgtg cgctggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aatgaccgtc tttttgaaac ctggcgatct
      901 gctggttgcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 actggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttgggtgccg atctggtgtt
     1201 gcattcatgt acgaaatatc tgaacggtca ctctgacgtg gtggctggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgtatgga
     1381 gctggcgcag cgcaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgggc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatttcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacttg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtgggccacg gggaagtatg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gtcagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct agtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaat
     4021 ggcaaagcgc gtgtgggcgt ggaagcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccatgcgata acgtctttgc catcgaaagc cgctggtacc gcgataatcc gctggtgatc
     4141 cgtgggcctg gcgctgggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtc
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatga agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aatggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ctgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagccg gaaatgtatg cttctgacct ggtgacgctg
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgctcaac ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt ctttgatgtc gaaagctacc tgcgttttcg tgaccgctgc
     5161 gtatcggcgg gcattgatgt ggaaattatt ccggggattt tgccggtatc taactttaaa
     5221 caggcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcacaaatg
     5281 tttgacgggc tggatgacga tgctgaaacg cgcaagctgg tgggcgcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acactgtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgtaaa aataaggttg tcagctttgt tttcgccctc cttccttgaa
     5581 gaataggaat ctaaaaccct tttttataaa gcagttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatattcata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggcctgccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgacc caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgtgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct tattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaatgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 cctgttcaag tatgagtggg tacagacccg cagcccggct ggcgcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gtttgatccg tcgaagaaac gtaaaccgac
     6901 gatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tttggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct
     7621 ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg accgacgaat cgaaagagct gtttgaaggc cgtgaccgcg aaaccggcga
     7801 agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtggctgaa gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     8041 actacacact ggatctctca tgtctgtcgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgtct
     8581 gtatgcccgt catccgcgcg tggatttatt gtcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccaa tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt agagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtaggagtt
     9721 tcagattact ggcacaga
//
LOCUS       DAZFDP010000168         9716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFDP010000168 DAZFDP010000000
VERSION     DAZFDP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..268
                     /locus_tag="ACHJ1G_004257"
     CDS             <1..268
                     /locus_tag="ACHJ1G_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFO4766011.1"
                     /translation="EAADFSDALMWLKDGKKVARRGWNGENQFCWLVPEGQYPARMEA
                     IKGYFPGDLVPYGAYFALKNAQGVVVPWVPSVGDLLACDWFVVE"
     gene            275..1057
                     /locus_tag="ACHJ1G_004258"
     CDS             275..1057
                     /locus_tag="ACHJ1G_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766012.1"
                     /translation="MENTKAIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTIMC
                     HLGFTEEVSKKHGVAIWRTMDTGMRRFITARTPGMTMMDLMQIAPLFECEPLDVFSNP
                     ALCQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIKSD
                     LKRAILEASCRVVGLGKQSYVSFPAGHEGPAEILIMDADLLWQIQFLIGKSIIRAEEL
                     DQYITCTMTDEVKSVAIANARNLCRAALTELQENTTEEVESD"
     gene            complement(1087..1404)
                     /locus_tag="ACHJ1G_004259"
     CDS             complement(1087..1404)
                     /locus_tag="ACHJ1G_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766013.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVEGGDEDDDDDDDDFGDHVDQ
                     IVY"
     gene            complement(1394..4381)
                     /locus_tag="ACHJ1G_004260"
     CDS             complement(1394..4381)
                     /locus_tag="ACHJ1G_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766014.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAQSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEHLDQLLKRFSYNPQTRWQMNQRSMGSVYEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQSGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            4481..5689
                     /locus_tag="ACHJ1G_004261"
     CDS             4481..5689
                     /locus_tag="ACHJ1G_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4766015.1"
                     /translation="MKQQVSFKFRLKPDGQQECQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTETEWLIDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEISTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHFRIANIRRDYLHKVTTIISKNHAMIVIEDLKVKYMSK
                     SAAGTISHPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWHGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(5729..6094)
                     /locus_tag="ACHJ1G_004262"
     CDS             complement(5729..6094)
                     /locus_tag="ACHJ1G_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HFO4766016.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGVSGGSSDEEKQPSEHDGVPKLIADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(6091..8010)
                     /locus_tag="ACHJ1G_004263"
     CDS             complement(6091..8010)
                     /locus_tag="ACHJ1G_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766017.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KMGDVAQNGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLIAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLVDQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGGHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAYPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            complement(8012..8614)
                     /locus_tag="ACHJ1G_004264"
     CDS             complement(8012..8614)
                     /locus_tag="ACHJ1G_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HFO4766018.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(8601..9044)
                     /locus_tag="ACHJ1G_004265"
     CDS             complement(8601..9044)
                     /locus_tag="ACHJ1G_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4766019.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(9041..9370)
                     /locus_tag="ACHJ1G_004266"
     CDS             complement(9041..9370)
                     /locus_tag="ACHJ1G_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4766020.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            9300..9635
                     /locus_tag="ACHJ1G_004267"
                     /pseudo
     CDS             9300..9635
                     /locus_tag="ACHJ1G_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         2390 a         2551 c         2236 g         2539 t
ORIGIN      
        1 tgaagcagcc gacttttctg acgcgctgat gtggctgaag gatggcaaga aagttgctcg
       61 acgcgggtgg aacggcgaaa accaattctg ctggctggtt cctgaaggac agtacccggc
      121 acgaatggaa gccattaagg gatatttccc cggcgacctc gttccgtatg gtgcttattt
      181 cgccttaaaa aatgcacaag gtgtagttgt tccgtgggtt ccttctgtag gcgacttact
      241 ggcatgtgac tggtttgtag tggagtgatt taacgtggaa aatactaaag caattcaata
      301 ccgcctgcgt aatggccaga gtgtcgaagt gaccatcaat aatgatggag tgccaggcga
      361 aaaggtttct atctctgatc tggctatcga aaaaaccatc atgtgccacc ttggctttac
      421 tgaagaagtg agcaaaaagc atggtgtagc tatctggcgc acaatggata ctggaatgcg
      481 cagattcatt actgctcgta ccccaggtat gaccatgatg gacctcatgc agattgcacc
      541 gctgtttgag tgtgaacctt tggatgtatt cagcaatcca gctctctgcc agcagttata
      601 tggtgagatg aaactcgcgg ttacccccat tgtgctgcat gaaggatcgc ttgctggcgt
      661 gtggaaagtg gagcgtattt caagctacat gcctttccat gtcaacggcg taatcactgg
      721 tgaaaatcaa cctgtttccg ttataaagtc agacctcaag cgcgcaattc ttgaagcaag
      781 ttgtcgagtt gtcggcctgg gcaaacagtc ttatgtttcc ttcccggctg gccatgaagg
      841 cccggcagaa attctgatta tggatgccga tctgctatgg caaatacagt ttctgattgg
      901 caaaagcatc atccgcgctg aagaactcga tcagtacatt acctgcacga tgacggatga
      961 agtcaaaagt gtggctatag ccaatgcccg gaacctatgt cgtgctgcat taacagaact
     1021 gcaagaaaac accacggaag aagtggaaag cgattaaaaa aaatcccgcc gattggcggg
     1081 atttcttcaa tatacgatct ggtctacatg atccccaaaa tcatcgtcgt catcgtcgtc
     1141 gtcctcatcg ccaccctcta ctgctggcca atcaacaaac cagccagcgt aaagatgcag
     1201 cgttcggaga acatcacttg cgggagcatc aagggtgtta acgaatccca tatagctatt
     1261 gggatttgcc ccagctatgg cttcagcgat catgtcctcg gtaatgtcac cggagataat
     1321 gcttaaacgc ccggaaactt cttcattatc atcaaattcg ataatggcat ctccgcctaa
     1381 tggcgctgcg attttaatct gcattattta gctcctttgc cacacctaat aacagttcca
     1441 gcaatccgtc accattcatc agtgatgcgg cagcggcctc tttgtcatga tacaactgaa
     1501 gagccataga gaatacttcc gttgctgacg ttttggaaat agtcggtgat ttctgccgaa
     1561 ttttcccggt gttacttact gaggctggcg ggtatacctt cgccatataa atattactca
     1621 atcgggatct gaagcaccat tcaggcttgc cacgcccacc gatattaacg aaagatggct
     1681 tatccccttc aacattggcc ttcaggaatg accgggcttt ctctaacaaa ccaggattac
     1741 tgtactcaag atgatggccc agctcgtgcc acagtgcact tgcattttca tcgttcaaat
     1801 tgacagcaac aacaccatta agatttgcat atgcccttcc ctggtggtga accacctttg
     1861 ataaggtcga aattttaccg ccggtcaggc gataaatatc agcaagttcc ttgcgcaggt
     1921 ctatcccacc actctgtcca gcgcgggctt cttccacttc ttccgtgata aaagagtcgg
     1981 cccactcaag agctttttct tcagatacgg atgagttggc tatcgcacta ttcatggcag
     2041 ataacacttt ctcatagacc gaacccatac ttcgctgatt catttgccag cgtgtctgcg
     2101 ggttatatga gaatcgctta agtagttggt caagatgctc aagttcttct tcgctgacat
     2161 actttttagc ctcaccaata atgccgggga gaatattgcc gttaggatta aacgctcgcg
     2221 aaaggaagag tttcagcgcc cccatgccct ccgatgcttc aatatcacca ataacacggt
     2281 taacaatggc cgcactcttc ggattagcat ccgccaacgc tctggctacg atttgcaggg
     2341 acgatacgac ctcacgctgc atatcagtcc tgatctcatc aataaactct ggcgttatgc
     2401 cgtgctcttt aaggatatcc ctgccttccg ccgttacccc atcgatatca ccgacatgtt
     2461 tattaacccg actttgcaat gccttaaatg ccttcagaat tccacgggca tcatcagctt
     2521 tactaacggc cttcctgaat gctggcaaga agtctgagtt aacctcattt tgttgatcgg
     2581 cccactgaat ggaggcttcc ttcatctcgt ccagagtcag atctcccaac gcggtatggt
     2641 ctgtgaatat gagcgataac ctctgaacca tttctgccaa tggtgatgct gattgcgccg
     2701 cgctaaggaa tgctttcacc ctggttgggc gaatggaaaa ccagtcaata gctggtggca
     2761 tatctccgtt ttttatcgcc tgcgctatct cgtcaaagcc atcgcgccca agggaggatg
     2821 cgtgatttaa caagccgcga agtaacgaat tgctgatacc gaataatcgg caccattttt
     2881 tcacgtcggc aacaggcatt cgaacaaaat gcgcaagcac ttgaacaagc tgttcatcct
     2941 ggggatctgt acgggaaagc agcctgatca gatgaataat gtctttgatg ccggatgccc
     3001 gatgtaatag caagctggta tatggagcaa caccgttgta actaccgcca gaagctgact
     3061 cgaaaagacc gccagatatc ccttgcatac cttcgttttc cagttcctga gatacttggc
     3121 gaaggatatc ctgtaacgac acatcgccgc cgccaaacat atccccgagc gcctggccct
     3181 ggtgctgtaa ctcatcattg atacgttgcg ccatcaactt aaaggcggtg gccatacgct
     3241 tcgcactacg gttattcgcg acgatgaaca acgcgagtgc tttcacttcc ggggccgttt
     3301 cgctgaacat atccccctga gcaataacat cggtaatatg ctggcctgac tccttcgatt
     3361 gccttaccag gtctaccgca tctttcaatg ccgccagcgc ctttttatcg aggctatccg
     3421 ctgtctcaat gccatcaaca atagttgtca cagcctgctt gtgcgcttct cctgataaag
     3481 cctgcatctg gacaaaatca ttggctgccg cattaagcgc cgtcagaaca ttacgcatat
     3541 ccggatcagg ttcttctgca accatcctta ccaggcgcgc atccttatat gccttggcaa
     3601 agatcgcgtt ttgtatacgg tctacaagtt gccgtgttgg tcgcccatct tccgttacaa
     3661 ggccagcagc ctgtgtggca ccaacttgcg tcatgaatcc gcgaataaac gcgtcattac
     3721 tgcggctaag cagatctcca ctttctgacg ggttaaacaa cgccatcatc gccggtgtta
     3781 tgctgtcggc atcaacaaaa gccttttcac tggctgccat ttcctggaga tcagaaatat
     3841 ttgagtcctt ggcaaactga acacggtcaa ccttcgttaa ccggcggcgc accagtaccg
     3901 gagccgtcat tgattcaacc ttttcaggcc gtatgccgaa ttcggtcgca tgttcaatca
     3961 ggtactcccg ataccgatcc gcattgccgt cctgataggc tttaatgatc cccatggtcc
     4021 gcccattacc tgactcaacg gcattgtcct caccaattat tggcgcgcca tggctggata
     4081 aaccggaatc agtaagctga gcaggccgca aatctttgga tatctggtta acctgaagaa
     4141 ggctggatgc gcgggtccgg tcgcgcggct gaagttcctg gggatagtca ggattaattt
     4201 tcccatccag agtattggat accaaaagag ctgaggcatc gacgatatca aacgctgttt
     4261 ttacctcgtc tcccttcgct gtcaccacat acgaaacccg cccataatcg ggcaggttct
     4321 ttagcagctc gatcagcgtt tctatgctgg tggccattac cacctgatcg cttaagctca
     4381 tccctgttac gccttatcca atgtgccgcc ccctacgctc ctcatagtgg ggatataagg
     4441 cacagttttc ctcccggcta tttttgctta aaatagtgac atgaaacaac aggtgtcatt
     4501 caaattccgg ttaaaaccag atggtcaaca ggagtgtcaa atgaggcgct ttgccggagc
     4561 ttgtcgtttc gttttcaacc gtgctctggc gcttcagaat gagaatcatg aggccggtaa
     4621 aaaatacatc ccttacacga aaatggcttc ttggttggtt gagtggaaaa aagacactga
     4681 aaccgaatgg cttatagatt ctccctcaca gccattgcaa cagtcactga aagaccttga
     4741 gcgggcttac aaaaacttct tccagaatcg ggctgctttt ccccgattca aaaagcgggg
     4801 acagaatgat gcatttcgct acccgcaggg cgttaagctc gatcaggaaa acagccgtat
     4861 ttttctgccg aaactgggct ggatgcgcta ccggaatagc cgtcaggtca cgggagttgt
     4921 gaaaaatgtc actgtcagcc agtcctgcgg taagtggtac atcagtattc agacagaaag
     4981 tgaaatatcc actccggttc acccttcagc atcaatgatc gggctggatg ctggcgtggc
     5041 taaactcgcc acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaaaa
     5101 ccagaagaag ctggcgagac ttcagcgaca gttaagccgc aaggtcaaat tcagcaacaa
     5161 ctggcagaag cagaaacgca aaatacagcg actgcatttt cgtatcgcaa atatccgcag
     5221 agactacctt cataaagtca caacgatcat cagcaaaaac cacgcaatga ttgtcattga
     5281 ggatttgaag gttaaataca tgtcaaagtc agcggcggga acgataagtc atccgggtcg
     5341 caatgtccgg gcaaaatcag gtttaaaccg ttcgatactg gatcagggct ggtatgaaat
     5401 gcgccgccag cttgagtata agcagctctg gcatggcggt caggtactgg cagtgccgcc
     5461 agcgtacaca agccagcgtt gcgcgtgctg tggtcatacc gcgaaagaaa atcgcctgtc
     5521 acaaagtaaa ttcagatgcc aggtatgtgg atatacagcg aacgccgatg taaacggcgc
     5581 tcgtaacatt ttagcggcgg ggcacgccgt acttgcctgt ggagggatgg tgcagtcagg
     5641 ccgcccgttg aagcaggaac ccaccgaaat gattcaggcg acagcctgaa cgtagcagga
     5701 atccacgccc cgaagggcgt ggaggatgtc acgctgcctc tttaatgttg gcggctatcc
     5761 atgccgccgt gtgctgttta acttggtcca ggtcgatgta tgtgccaaca tattgactca
     5821 agtcctgtaa cgtaccgata aatgcatcag tgctctgatc gacgaattta tcagccagga
     5881 aatcagcaat cagttttggc acaccatcat gttccgaagg ttgtttttcc tcgtcactac
     5941 tgccgccgga cacgccgtac cccatctgtt gcatgatctg gtcaatttca tcgctgatat
     6001 ccagcaactc catgccactc gcggtagccg ctttggacat cagagcatcc aacttatcgc
     6061 tgagatccat taactcaata gctgatagtg tcatgccgct acccccgctt tctggattgc
     6121 taccagcaga tcagccaggt ggcgagcagc gccattaacc agctcttcgt tttcctcaaa
     6181 acgcccggca gcctgaaggg ctgcaatcgc ttcccggaca ttaccccggg cgttacggat
     6241 ctccgccatg tcagtgcttt gcatatccat cacgttattg agatattcaa tggctttatt
     6301 agcctctgca tctgcttcgc taaccgtttc atcaggctgt gccggggcag gttctggctg
     6361 agtaatctca ccgacttcgg cctgcaatgc attgatcatg ctctgcacca ttttctcggt
     6421 gccagcgccc cccggatacg caatattggg gaaagttttt tgaaactgag ttttcagcat
     6481 tacgcggaac tcgtctggtg agctggtggc cagctccaga gctttttgtg catatttgcc
     6541 aaacggacca ttagtaagtg tcttcgccag gaagtcgaaa gaatcctcgc gaggcaataa
     6601 cttcaggtcg tactcactca tttgctgatc agaaagcggg gtatcgtaag tagcaatgcc
     6661 gtagcgtgca tattcataat acgggtcacc ttcatcaggg cgcggcagaa ttgctttgtt
     6721 accttcaggt attgcgccag gggccgccgg acgcatttgc agggcatatc gatatgcacc
     6781 tacagagact tctggttcag gcgaagagct accggtatcc tccgctggtt caggttcgac
     6841 gttttccggt ttatgttctt ctggttggac caggtattcc gatacattac ccgctttata
     6901 ggctttaaac agcttgccga tcgcatctgc catgtccaca ccctgtatgg atttagcctt
     6961 gatcatgtac acgctgccat ccgaatcggt taactggata tacccttcac cgtcctcaat
     7021 gaattgcttc attgatgcac cattactgag cgtcacttcc ccgttcatat gcatacgatt
     7081 tttgatactt gcaaggcgat ccgtcatcgc gcgagagtgt ccaccagtca tccccgcagg
     7141 agcaatggtt tcgcgcccac cagttcgatt gagctgatca atctccgtct gcaaacgctc
     7201 attctcttca taaagagaat ccgcttccga tgcaacagcg ttaattttct gctccagatc
     7261 taccttctgc ccttctaccg ctgccacctg atccacgagg tcgctcatgg catcctcttt
     7321 ctggtcactg tcagcctgta gttgggttat ttcatcaaca agggcttttt tcttcttctg
     7381 cgcacgctgg aattttgccg agtttttctc tgcaaggttg gcaagtttca tggtgacctg
     7441 cgccagcgtc atatcacgtc cactcatcgg agcaacggta tgagtaacgt cttttttatt
     7501 cagtaagaac tggaaagcaa tcagcgtatc gctattggtg atccggtttt ccgctgtcgg
     7561 gctatgaaac agaatgctga tagtctgacc atcactgagc ggaataatgg ctggcagaac
     7621 cggcagcccg ttaacgttac gtgcccggcc aatttcagcc ccgccgatcg cgcgtgcacc
     7681 gttctgggcc acatccccca ttttatcact ccccgcagag attccggtac cattcagctt
     7741 ctggttcaat gcccggacaa atgcctgcat ggtccggtgt agctgcaaac gagtagaact
     7801 aatcgcctcc agtaaatccg tagcacacca gtggatcggc gtgtcataga agaacgtagc
     7861 ctcgatttct tccagggtgt tggattccgt catcagatag cggtcctcac cggccattaa
     7921 tgcgcgatat tcatcatcag tcactggcgg gggaagcacg tcaagcccag gcttgatcgt
     7981 caccccttta ttgatattga actgttccat gttaatttcc tgctttcagt tgcttaagac
     8041 ggcgtttgag ttcgccattt cgggcctttt cgttattgag tcggcctgtc tccttatcca
     8101 gcttcgcccg caaatcagtg atctgctgtt gattgaaaga caccgagttc tgcgctgatt
     8161 tataagcggc aaccacctga gcattccgct gttttgcctc ttgcaggcgc tgaaagttgg
     8221 attttactgc cggtttcttg tctaccggat tggcaacacg tttcgctttg gcgatcagtg
     8281 atttctggaa ttttgcggag tttttgcggg ccgcttgccc catgacggta ccaagcgtct
     8341 tgatatccgg cgactgagcg ttaggaatag cttttccatt cagcctcaca gacgatatat
     8401 cgccagtatc gtttacctgt atggcaagaa tttgtccgtc gttaagaacc agctttgcgg
     8461 ttttaacttt aacgccatct ttcgttgttg cgcggttgct ggagtcaacc tcaattaccg
     8521 taacaccggt tttattgatc gccgcgataa gggatttcag ccccttttca ttaacctggt
     8581 caaaatcgac cgttgcatac ttatttttcg tcatctgaca catcctgtgc gagatttatt
     8641 acgtaacttc tgcggatttg ctgagtaaca gggaaaatcc gatacaacgg gttaatgaac
     8701 gagtcgccat gcgtaaccat gacgttgaaa tgccacagcc gttctccttt acccatatat
     8761 tcagtgggta tgtacaacca ttcactgttt tcgccctgtt cagccgacgt cagacaacgt
     8821 tgttcgcctt caatcactgt cgttggcttc tgaacatcgc ggatccaata tctgaccgtt
     8881 gcgccgcgca aaaacgggaa tttagaccgg tatttgaacg gcacccggat gaaacccggt
     8941 ttaatttcca catcaccaag ttctaaatgc gtgatgtcct tgcgttttag caaatagcga
     9001 tcggctaagg ctaacgcaag agcgcataca ccccagccaa tcatttccca cctccctttt
     9061 tcaccaaact tgtaagaaca ttcagaatgc tatcgatatt cactcgtttc atccctgaaa
     9121 tcacctcatg accgttattg ctggctatcg ttaccattaa gtacgtaatt gataactccc
     9181 agccctcgtg ttgccccaat aggtacgcca ccgcgccagc tgtcactgca acaaagatct
     9241 ccgtaaccaa tcccaacaaa ttgccagact ggcgaccgtc tcggacatcc atcaggaacg
     9301 tgcctatccc accaattact gaaagcagga gcgcaatagc aactggagct aattcctgtg
     9361 tgtcaagcac aagttccctc ctacgttgtc aggaggtaat ggtatgcaaa gtaacttctc
     9421 aaccggtcat gcaaagacaa caagcggaaa aagaaaagca aagcctgcta cagctcgtca
     9481 gggataaaac tcagctctgg gactcacagt tacggctagg cattatttcc gttcagggta
     9541 aacaaaaatt aaccgagtgg attctctatg cgcagaaagt cgaatccacg gatacctcca
     9601 tccttccagt aacgtttccc gaaaagccgg aatgaggaaa ggcccggaat cgggccttaa
     9661 ttaatatcta taagctcaac aaataagagc taacaatttt gttaatttcc tctgga
//
LOCUS       DAZFDP010000169         9538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFDP010000169 DAZFDP010000000
VERSION     DAZFDP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..204)
                     /gene="cspA"
                     /locus_tag="ACHJ1G_004268"
     CDS             complement(<1..204)
                     /gene="cspA"
                     /locus_tag="ACHJ1G_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4766021.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVT"
     gene            complement(485..775)
                     /locus_tag="ACHJ1G_004269"
     CDS             complement(485..775)
                     /locus_tag="ACHJ1G_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4766022.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1209..1919
                     /locus_tag="ACHJ1G_004270"
     CDS             1209..1919
                     /locus_tag="ACHJ1G_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4766023.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1969..2943)
                     /gene="ghrB"
                     /locus_tag="ACHJ1G_004271"
     CDS             complement(1969..2943)
                     /gene="ghrB"
                     /locus_tag="ACHJ1G_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4766024.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3047..3706)
                     /locus_tag="ACHJ1G_004272"
     CDS             complement(3047..3706)
                     /locus_tag="ACHJ1G_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4766025.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3859..6192
                     /gene="bisC"
                     /locus_tag="ACHJ1G_004273"
     CDS             3859..6192
                     /gene="bisC"
                     /locus_tag="ACHJ1G_004273"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4766026.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASLQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6161..6601)
                     /locus_tag="ACHJ1G_004274"
     CDS             complement(6161..6601)
                     /locus_tag="ACHJ1G_004274"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4766027.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6598..7161)
                     /gene="tag"
                     /locus_tag="ACHJ1G_004275"
     CDS             complement(6598..7161)
                     /gene="tag"
                     /locus_tag="ACHJ1G_004275"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4766028.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7319..8017
                     /locus_tag="ACHJ1G_004276"
     CDS             7319..8017
                     /locus_tag="ACHJ1G_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4766029.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8246..9448
                     /locus_tag="ACHJ1G_004277"
     CDS             8246..9448
                     /locus_tag="ACHJ1G_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4766030.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2246 a         2341 c         2485 g         2466 t
ORIGIN      
        1 ggttacgtta ccagctgccg ggcctttagc gccgctttcg atggtgaagg acactttctg
       61 accttcgtcc agagatttgt aaccatcgtt ctggatagca gagaagtgta cgaacacgtc
      121 tttagagcca tcgtcaggag tgatgaagcc gaagcctttg tcagcgttga accattttac
      181 gataccagtc attttaccgg acatagtgta ttacctttaa taattaagtg tgcctttcgg
      241 cgatatggcg tgctttacag attttgaagc gttaaaggaa tgtgcactac gaggggtatc
      301 aacgataact cttgaaggga cttgccttac tacactgctt taatggtctg tacgtcaaac
      361 cgttgatgtg cattaagcca cgcattggcg ggtgatgcaa caattatttt tcatatttat
      421 gattaatcgg ttggaaaacg gtcctgtcat caggaccgta aacagcaata aagtggataa
      481 aagtctattc catcaactgc ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt
      541 ttagttcggc acttgaaggc ttcacgcgtc tggattccca ttcctttacc atggcgactg
      601 atacgcccaa aacccgggcg aaatcatcga tttttaaccc tgtccctttt cgtaactgct
      661 caatttcggt acaggacgtt gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa
      721 aaacaatctg ttccaggctg ctcaacagct catgcattgg atctttatat tccattgaaa
      781 actcctcaaa tcacactgcg ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg
      841 aaaagcagac acagcggggt gaagtcaggg catgctctgg ctatgattcg attcagccag
      901 tcatttagcg tgacttatag ccacctaacg atctgataaa tgccgatata tctcttacca
      961 ctcctggttt gtaaagattt cgaaaatcta accttctgcg aaggcgtaaa tctctggcta
     1021 caaaaaaagc aacggcacta tcatttttta acaatgaaac aacaaattaa ccttgtttca
     1081 ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg
     1141 gactatcctt gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga
     1201 gtaagaaaat ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat
     1261 taatggtagt tagcctgagt gggtgttttg ataaagaagg cgatcagcgt aaagcgttta
     1321 tcgacttcct gcagaataca gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg
     1381 atcagaaaaa acagtttggt ccttttgtct ctgattacgc gattctgtat ggttattctc
     1441 agcaggtgaa tcaggcgatg gattccggtc tgcgcccggt cgtagacagc gttaacgcaa
     1501 ttcgcgtacc gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg aacggttcgc
     1561 tgggtgtgct ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg
     1621 cgctaaaaca gaccgatgat ttgaaaccgg tctttgatca ggcattcacc aaagtggtaa
     1681 cgacgccagc tgatgcattg caaccgttga ttccggctgc acaaactttc acgcaacaac
     1741 tggtgatggt tggagactac attgctcagc aaggtactca ggtgagcttt gtcgcaaatg
     1801 gcatccagtt cccgacttca cagcaggcaa gtgaatataa caaactgatt gcgccattac
     1861 cggcacaaca tcaggcgttt aatcaggcct ggactacagc agttactgca acccaataaa
     1921 gagtaaaagc ccgagcgatt atcgggcttt acttcacgca gtcgcggctt agtccgcgac
     1981 gtgcggattc acacagttct tctcaacctt tccttgtaac gcatcaatca aattatccac
     2041 ggcacaggcg gccatgccat aacgcgtctc atgggtggca gatccaatat gcggtactgc
     2101 gacgacgttg gccattgaga gcaacggcga atctacggac agtggctctt gttcgaagac
     2161 atccagcccg gcggcgtgaa tttcgccttt ctgcaatgca gcaatcagtg cattttcatc
     2221 aaccaccggg ccacgtccgg cattaatgaa aatggcggag gatttcattt tggcgaattg
     2281 ttctgcgcca aacagatgat gcgtctcatc agttaacggc aggatcaggc aaacgaaatc
     2341 tgactcttgt aacagagtat ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc
     2401 tttatggtgg cggcgcgcgt tatagaggat gggcatgttg aagccaaagt gcgcacgttg
     2461 tgccagcgcc ataccgatcc gtcccatccc gacaatgccc agtgttttat ggtgaacgtc
     2521 agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta cccgttctgc
     2581 tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat cggcgacggt
     2641 ttctgttaat acggttggcg tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac
     2701 atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg gcattttttc
     2761 cagcaatgcg gcatcaacat tctcgtttga acccagtaaa ccttcagctt cggcaaaaat
     2821 tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg
     2881 ctcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa cggacggctt
     2941 catgcttctc tccattcact gataaggcga tcaccttagc ctgaaatatg atgaaggaaa
     3001 actgaagaat gccaggtgat ttctgcatca cctggcatga aagggattac agcgggctta
     3061 aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg ctggcgattg
     3121 ggttagccgg gccaaggccc tgagtacgga tgcggctggc gtccacgccc tgggtgatca
     3181 acgcgctggc aacggaatcc gcacgttgct gggagagacg catgttcagg tcgtgaccac
     3241 ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct ttcagcacca
     3301 ttgccacgcc ggtcagggtg ttagcgcccg caggtttcag ggtcgcgctg ctgctgtcga
     3361 aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt acgctaacac
     3421 cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa taacccacgc
     3481 cgccgcccag agctgcgcct gctgctgcgc caatcagcgc gcctttaccg cgatctttct
     3541 tcgaagaaga gagcgcacca atacccgcgc ccacgagaga gcccagacct gcgccgatag
     3601 cagatttacc tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca gataccgcca
     3661 gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt aatccttaat
     3721 aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag
     3781 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
     3841 ctttcaggag ttttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
     3901 cattgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcatggcgcg
     3961 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
     4021 acacgggtac gatttccaat ggtgcgcaaa ggctttcttg cgtcaccgga aaatccgcaa
     4081 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
     4141 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
     4201 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
     4261 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggcgc ggcacaggcg
     4321 atcatgccgt atgtcgtggg tggtagcgaa gtttatcaac agcaaaccag ttggccgctg
     4381 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
     4441 attgcgtgga atgcatctga tgagcagggg ctttcttact tttccgcact gcgtgacagc
     4501 gggaaaaagc tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     4561 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     4621 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tgacgcgttg caccacaggt
     4681 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa
     4741 tgggcggcag agatttgtgg ggttaacgca gcgaaaatcc gcgagctggc ggctcttttc
     4801 caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca gtttggtgag
     4861 caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc
     4921 ggcggttttg gtctttctta tcattttgcc aatggtggta accccacgcg gcgttctgcg
     4981 gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct
     5041 gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg
     5101 aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     5161 catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     5221 gaatgcttct ggacggcggc ggcgaaacac gcggatatcg ttctgcctgc gactacctct
     5281 tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg
     5341 aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg
     5401 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa
     5461 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagcctgca ggttgaattg
     5521 ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac
     5581 agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa
     5641 accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat
     5701 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa
     5761 cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt
     5821 tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat
     5881 gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag
     5941 atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa
     6001 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac
     6061 gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacggcg
     6121 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     6181 gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt
     6241 gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     6301 tcagatgggg atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga
     6361 cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac
     6421 cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     6481 gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     6541 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     6601 tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     6661 ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc
     6721 cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg
     6781 tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg gttcgccgtt
     6841 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg
     6901 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac
     6961 cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt
     7021 gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca gttttttact
     7081 gtcagtttca ggcacgcccc attcattatc atggtaggca atataaagcg ggtcctgact
     7141 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     7201 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     7261 atccttcatg caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     7321 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag
     7381 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     7441 cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt acaacgatat
     7501 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     7561 agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga
     7621 acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     7681 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     7741 tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     7801 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     7861 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     7921 tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta ataatagcga
     7981 ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca
     8041 ttgaataaca acagttgtgg cgatagtggg tgctaactta ccaaataata aatttggtga
     8101 ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc
     8161 atgccgtttt tccccaggca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc
     8221 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct
     8281 gacactcatc ggtactatca ttacccagtt tgcactgggg tcggtttata cctggagcct
     8341 gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt
     8401 cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg
     8461 ttttggcgtt aaacgcgtca ccatggcttc cggcattttg ctgggattag gcttcttcct
     8521 gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct
     8581 ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccggagcg
     8641 taaaggtctg atatccgcgt tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa
     8701 atttatcgac acgcagctgc tggaaacggt gggtctggaa aaaacctttg tgatttgggg
     8761 agcgattgcg ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca
     8821 ggaagtgaaa accagcaatg gtgtggtgga gaaagattac acgctggcag agtcgatgcg
     8881 taaaccgcag tactggatgt tagcggtaat gttcctgacc gcctgcatga gcggcctgta
     8941 cgtgattggg gtagcgaaag atatcgccca aagtctggca caccttgatg tggtttccgc
     9001 agccaatgca gtcactgtta tttccatcgc caacctttca ggtcgtctgg tgctgggtat
     9061 tctgtctgac aaaatcgccc gtatccgtgt tatcaccatt ggtcaggtga tatcgctggt
     9121 gggtatggcg gccctgctgt ttgcaccatt gaatgcagtg acgttctttg cagcgattgc
     9181 ctgcgtggca tttaactttg gcggcactat taccgtcttt ccgtcactgg tcagtgagtt
     9241 ctttggcctc aataacctgg cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg
     9301 tagcatttgt ggttcgatta tcgcctcact gtttggcggc ttctatgtga ccttctacgt
     9361 gattttcgcc ctgctgattc tgtcattggc gctttctacg acaattcgtc agccagagca
     9421 gaaagtattg cgtgaggcgc atatttagaa atatgttgct actgaaataa atgcgaggag
     9481 ggagacttca aagtctccct ttttttatgg acgcatatta aaagaacaat aatattta
//
LOCUS       DAZFDP010000170         9306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFDP010000170 DAZFDP010000000
VERSION     DAZFDP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(102..722)
                     /gene="sodA"
                     /locus_tag="ACHJ1G_004278"
     CDS             complement(102..722)
                     /gene="sodA"
                     /locus_tag="ACHJ1G_004278"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4766031.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1007..2041
                     /gene="rhaT"
                     /locus_tag="ACHJ1G_004279"
     CDS             1007..2041
                     /gene="rhaT"
                     /locus_tag="ACHJ1G_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO4766032.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(2038..2886)
                     /gene="rhaR"
                     /locus_tag="ACHJ1G_004280"
     CDS             complement(2038..2886)
                     /gene="rhaR"
                     /locus_tag="ACHJ1G_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4766033.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2960..3797)
                     /gene="rhaS"
                     /locus_tag="ACHJ1G_004281"
                     /pseudo
     CDS             complement(2960..3797)
                     /gene="rhaS"
                     /locus_tag="ACHJ1G_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            4085..5554
                     /gene="rhaB"
                     /locus_tag="ACHJ1G_004282"
     CDS             4085..5554
                     /gene="rhaB"
                     /locus_tag="ACHJ1G_004282"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO4766034.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5551..6810
                     /gene="rhaA"
                     /locus_tag="ACHJ1G_004283"
     CDS             5551..6810
                     /gene="rhaA"
                     /locus_tag="ACHJ1G_004283"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4766035.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            6893..7717
                     /gene="rhaD"
                     /locus_tag="ACHJ1G_004284"
     CDS             6893..7717
                     /gene="rhaD"
                     /locus_tag="ACHJ1G_004284"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4766036.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            7789..8937
                     /gene="fucO"
                     /locus_tag="ACHJ1G_004285"
     CDS             7789..8937
                     /gene="fucO"
                     /locus_tag="ACHJ1G_004285"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4766037.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            8934..9248
                     /gene="rhaM"
                     /locus_tag="ACHJ1G_004286"
     CDS             8934..9248
                     /gene="rhaM"
                     /locus_tag="ACHJ1G_004286"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4766038.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2217 a         2376 c         2489 g         2224 t
ORIGIN      
        1 tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg
       61 atatgcggcc tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa
      121 cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg
      181 cggttctgga atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag
      241 ccggaagcgc cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc
      301 accgccagtt tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct
      361 gccgcttttt caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc
      421 gctttcaggt caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg
      481 ttagcgtgac cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc
      541 agtttggtga tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc
      601 gcgttggcgt tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc
      661 ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc
      721 atattcatct ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca
      781 ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat
      841 ggttttgcag gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
      901 agtaaatcga caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc
      961 atctcgtcgg agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat
     1021 tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc
     1081 gttcaaaaaa gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc
     1141 gtggattatt ctgccgtggg ccatcagcgc cctgttacta ccggatttct gggcgtatta
     1201 cagctcgttt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat
     1261 cggtaatatc aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat
     1321 cgccattggc attacgttga ttgtcggtac actgatgacg ccaattatca acggcaattt
     1381 cgatgtgctg attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct
     1441 gattggcgta gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa
     1501 agccgaagag ttcaatctga aaaaagggct ggtgctggcg gttatgtgcg gcattttctc
     1561 tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact
     1621 tggcgtcgat ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc
     1681 gatcattaac ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa
     1741 agccgacttc tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg
     1801 cgggttgatg tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc
     1861 gcagtatgac tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat
     1921 cgtcggactg gtgctgaaag agtggagcaa tgcaggacgc cgtccggtaa cggtgttgag
     1981 cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta
     2041 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
     2101 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
     2161 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg
     2221 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
     2281 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
     2341 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
     2401 atggcgattc agcaacatcg ccaactgccc gaacagcaac tctgccattt cgttagcaaa
     2461 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
     2521 ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc
     2581 cccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac
     2641 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc
     2701 acgggtaatg cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat
     2761 caccagctca caaaaatcat gtgtatgttc agcaaagaca tgttgcggat aacggtcagc
     2821 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
     2881 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
     2941 tacgttgaga agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg
     3001 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc
     3061 aatgataggc gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggccttca
     3121 tcagtcgcag gcggttcagg taacgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
     3181 gatgtagcgt tcgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
     3241 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
     3301 ggttcttctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aaagatctcg
     3361 cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc
     3421 tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc
     3481 agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga
     3541 tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg
     3601 cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata
     3661 cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc
     3721 gcctgaggga gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca
     3781 ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg
     3841 aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca
     3901 agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat
     3961 tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa
     4021 cgagaaggtc acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca
     4081 cattatgacc tttcgcaatt gtgtcgccgt caatctcggc gcatccagtg ggcgcgtgat
     4141 gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa
     4201 aaatgggctg catagccaga acggttatgt cacctgggat gtggatagcc tggaaagtgc
     4261 cattcgcctt ggcttaaaca aagtgtgtga ggaagggatt cgtatcgata gcattgggat
     4321 tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc
     4381 cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg caggcacagc aacaactcgg
     4441 caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca
     4501 gttgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct
     4561 gatgccggat tacttcagtt atcgcctgac cggcaagatg aactgggagt acaccaatgc
     4621 caccaccaca caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg
     4681 gagcggggcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca
     4741 ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc
     4801 cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac
     4861 ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc
     4921 caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg
     4981 cttatggctg cttcagcgag tgcttcagga acggcaaatc aacgatctcc cggcgcttat
     5041 cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt
     5101 tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc
     5161 gatcccggaa agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta
     5221 tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa gatttctcgc aactgcatat
     5281 tgtcggcggc ggctgccaga acacgctgct caaccagcta tgcgccgatg cctgcggtat
     5341 tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc aatatcggca tccagttaat
     5401 gacgctggat gaactcaaca atgtggataa tttccgtcag gtcgtcagca ccaccgcgaa
     5461 tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca
     5521 ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag
     5581 ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt
     5641 gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac
     5701 ccggaaggtt cgctgaccgg tgggattcaa gctactggta attatccggg caaagcgcgt
     5761 aatgccagtg agctacgtac cgatctggaa caggctatgc ggctgattcc ggggccgaaa
     5821 cggcttaatt tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc
     5881 aaacctgagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat
     5941 tttaacccct cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc
     6001 gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc
     6061 tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg
     6121 aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag
     6181 gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt
     6241 ggcattggcg cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc
     6301 agccgccaga cggcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc
     6361 gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagtcgtccg
     6421 gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt
     6481 gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc
     6541 tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg
     6601 cgtgcgttgc tggaacctac cgccgagctg cgcaagttgg aagcggcggg cgattacact
     6661 gcgcgtcagg cactgctgga agagcagaaa tcgttgccgt ggcaggcggt atgggaaatg
     6721 tattgtcagc gtcacgatac cccagcgggt agcgaatggc tggagagcgt gcgggcttat
     6781 gagaaagaaa ttttgagtca gcgtgggtaa acactgccag atgcggcacg agcgccttat
     6841 ccggcctaca gatcgtgcat ctgacaacac cccatttaca ggaacaccga acatgcaaaa
     6901 cattactcag tcctggtttg tccagggaat gatcaaagcc accaccgacg cctggctgaa
     6961 aggctgggac gagcgcaacg gcggcaacct gacgctacgc ctggatgacg ctgacatcgc
     7021 accgtatcac gacaatttcc accaacaacc gcgttatatc ccgctcagcc aacccatgcc
     7081 tttactggca aatacaccgt ttatcgtcac cggctcgggc aaattcttcc gtaacgtcca
     7141 gcttgatcct gcggctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca
     7201 tattctctgg gggttaacca acgaggccgt tccaacttct gaacttccgg cacactttct
     7261 ctcccattgc gaacgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
     7321 tgccaccaac ctgatcgccc tcacctatgt actggaaaac gacacggcgg tcttcactcg
     7381 ccaactgtgg gaaggcagca ccgagtgcct ggtggtattc ccggatggcg ttggcattct
     7441 gccgtggatg gtgcccggca cggacgaaat cggtcaggcg accgcacaag aaatgcaaaa
     7501 acattcgctg gtgttgtggc ccttccacgg tgtgttcggt tccggaccga cactggatga
     7561 agcctttggt ttgatagata ccgcagagaa atcagcggag gtgttagtca aaatctactc
     7621 gatgggcggt atgaagcaga ccattacccg tgaggagctg attgcactcg gtgagcgttt
     7681 tggcgttacg ccactggcca gtgcgctggc actttaatat cccaacagat atccggcggt
     7741 gtgggaaacg tcgccggaca gatggatttg aacttaagga gagtcattat gagttttatg
     7801 ttggcattac cgaaaattag cctgcatggt gctggggcaa ttggcgatat ggtaaatctg
     7861 gttgcgcata aacagtgggg aaaagcactg atcgttaccg atgggcaact ggttaagttg
     7921 ggattactgg acagcctgtt tatcgcactg gatcaatacg aaatgtctta tcacttattt
     7981 gatgaggtat ttcctaaccc cactgaagaa ctggtgcaaa aaggctacgc tgcatttcag
     8041 gatgccgcat gtgactacat tattgctttc ggtggcggta gcccaattga tactgcaaaa
     8101 gccgtaaaaa tccttaccgc taatcctgcg ccttctacgg cgtactctgg tgttggtaaa
     8161 gtgacaaatc ccggcgtacc gctggtggca atcaatacga ctgccggaac agctgctgag
     8221 atgaccagca atgcggtgat catcgacacc gcgcggcagg taaaagaggt cattatcgac
     8281 ccgaatatta ttcccgatat tgcggtggat gatgccagtg taatgctgga gatcccggcc
     8341 tcggttaccg ccgccacggg tatggatgca ctgactcacg cagttgaagc ttatgtttct
     8401 gttggtgcac atccattaac cgacgctaac gcactggaag ctatccgttt aattacgctg
     8461 tggctgccaa aagcggtaga tgatggtcat aatctggaag ctcgtgaaca aatggcattc
     8521 gcccagtacc tggcgggtat ggcatttaac agtgctggtc tgggccttgt tcatgcactt
     8581 gcgcatcagc ccggcgccac ccacaacctg ccgcatggtg tgtgcaatgc tatcttgttg
     8641 ccagttattg aaaactttaa ccgcccgaat gccatcgcac gctttgcccg cattgctcag
     8701 gcaatgggtg tcgatacgcg tgatatgagt gaagaagcgg caagtactga agcaattaac
     8761 gctatccgcg cattaagcca tcgcgtgggt attccggcag gattcagcca gcttggcatc
     8821 accaaagaag atattgaagg ctggttagat aaagccctgg ctgacccgtg tgtgccatgt
     8881 aacccgcgta ccgccagccg cgacgaggtg cgcgagctgt atctggaggc attatgatcc
     8941 gtaaggcatt tgtgatgcag gttaaccccg acgctcacga agagtatcaa cgtcggcata
     9001 accccatctg gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca
     9061 tttacctcga caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagagc
     9121 gctggaatgc ggttgccagt actgatgttt gccaacgttg gtggaaatat atgaccgacg
     9181 tcatgcccgc gaacccggat aacagcccgg tgagtagcga actgcaagaa gtgttttacc
     9241 tgccgtaatt gcctaatgtg ccggagacgc ctccggtaca atatttttaa tttcgatttg
     9301 aaaaat
//
LOCUS       DAZFDP010000171         9221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFDP010000171 DAZFDP010000000
VERSION     DAZFDP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..3946)
                     /gene="entF"
                     /locus_tag="ACHJ1G_004287"
     CDS             complement(65..3946)
                     /gene="entF"
                     /locus_tag="ACHJ1G_004287"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4766039.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3943..4161)
                     /gene="ybdZ"
                     /locus_tag="ACHJ1G_004288"
     CDS             complement(3943..4161)
                     /gene="ybdZ"
                     /locus_tag="ACHJ1G_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO4766040.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4164..5365)
                     /gene="fes"
                     /locus_tag="ACHJ1G_004289"
                     /pseudo
     CDS             complement(4164..5365)
                     /gene="fes"
                     /locus_tag="ACHJ1G_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5608..7848
                     /gene="fepA"
                     /locus_tag="ACHJ1G_004290"
     CDS             5608..7848
                     /gene="fepA"
                     /locus_tag="ACHJ1G_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO4766041.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7888..7986
                     /locus_tag="ACHJ1G_004291"
                     /pseudo
     CDS             7888..7986
                     /locus_tag="ACHJ1G_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8023..8643
                     /gene="entD"
                     /locus_tag="ACHJ1G_004292"
     CDS             8023..8643
                     /gene="entD"
                     /locus_tag="ACHJ1G_004292"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4766042.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8765..8917)
                     /gene="hokE"
                     /locus_tag="ACHJ1G_004293"
     CDS             complement(8765..8917)
                     /gene="hokE"
                     /locus_tag="ACHJ1G_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4766043.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         2237 a         2546 c         2436 g         2002 t
ORIGIN      
        1 aatcaaacaa ataaaaaaac ccataattat tttctgtaat tatgggttta tagatattat
       61 taatttacct gtttagcgtt gcgcgaataa tcggcccaat ttttacaaac gcccctggag
      121 agataatatc cacatgcgca caatcctgac gatagatatc cagctccgca atccacggcg
      181 accaggcacg ttcgggactc ataccttcct gaagtgtgcg ctcagcaaca aataacgttg
      241 cttttccatc aaacggaacg ctatgggcgg tggttaacaa acgcacagca tcagcgtagt
      301 tgccttcaat ggtggtaaac aactccgttg aagtacttcc ctgctgtgcc gccaggaagg
      361 cctcgcgttc gcggttaatc tccgccagca cttccgggtc cagaccatta gcctcttttt
      421 cctgccagtt ttgcgtctct ggcggccagg tatccagcaa accaagaaat gccacctgtt
      481 cgccacgggc acgcagtcgc gccgcaatac cctgcgccag cgtaccgcca agggaatacc
      541 ccagcaggta ataagggccg tgcggttgtt gctcaagtaa cgttgccaga tgcgcttcgc
      601 agacttcatc caggtttgtc gctgtctgca tgggtccatg agggcgcggc gactgaatgc
      661 caataatcga ccattgtgga tcgagataac gcgagagcac gctaaactgc caggcaaaac
      721 cggacgccgg atggaaacaa aacagcgtcg ggccattacc ttcacgcaac ggcagaatgg
      781 tttcgaatcc catgcgccgg gagctgtcct cttcaccatc aataatcgtt gccagtttgg
      841 cgacggttga cgcgaccatc acctgccccg gcgtcacctg acgagcaaac tgtcgactta
      901 actgcgctgc tagtttcatt gccagtagcg aatgaccgcc cagcgcaaag aaatcagcat
      961 cggcatcctg cacgtcacaa cccagcaacg acgcgaatgc cgcggcgata atcgtttcac
     1021 tgcccgcttt cggtgcacgt cccgacgctt gtgtcttcag ttcaggcaac ggtaaggctt
     1081 tgcgatccag cttgccgttg gcgctaagcg gcaactgcgg aagttgcagc agaacgaccg
     1141 gcaccatatg cggcggcaat gtttcgcgaa gctgtgcctg taatgcgctg gtatccaacg
     1201 gcagtcccga ttgcgacacc agatagccca ccaactgacg cgcatcacca ccggtggctg
     1261 ccgcctggtt aatcacacag gcgtgggtaa ctgcctgttc gacatccggc agcgcctgca
     1321 tcacgcgatc gatttcgccc agttcgatac gctgcccgcg aatttttagc tgatcgtcac
     1381 tgcgcccgag gtactccacc gcgccgttat ccagccagcg ggcgacgtct ccggtacgat
     1441 acatccgttc acctggggca aaaggatcgg caataaaacg gctggcggtc agatcgggtc
     1501 gtccaagata cccctgcgcc agttggatgc cggtgagata gagatcaccc gccacacccg
     1561 gcggcaccgg atgcatcatc gcatcaagaa tacgcagacc cgtattccat accggataac
     1621 caatcggcac actgctgccg cgcacctgtg ccagttcctc gccaaaagcc ggataccagc
     1681 tgacatctac cgccgcttcc gtcgggccat acaggttatg caacggcgcg ccggttaact
     1741 gttgccattc gcggcataaa tcagccggta aggcctcacc actacagaaa acctgtttca
     1801 acgtcgcgca attctggcga gcggtttgcg gcgtcagcga tgcaacaaat gccgccagca
     1861 tcgacggcac aaagtgcgtg gtcgttacgc catattcggc aaagaattgc tgcatagcga
     1921 gcgggtcacg gtgcgcttcc ggttcagcca tcaccagttt tgcccctgcg ataaacggcc
     1981 agaaaaactc ccacaccgag acatcaaaac tgcacggcgt tttttgggca acgacatctt
     2041 cgcctgtaag cgggtagtga ttttgcatcc ataacaggcg gttaacgata gccgtctgcc
     2101 cgaccattac ccctttcggc ctgccggtgg agccagaggt aaagatgata taagccgtgt
     2161 ggtgcggttg tgaaagttgc agcggcgcac tgccctgggg ggtaagcggg gcgttatagc
     2221 aaaggcttgt taaattggga acatcgctaa agcgcggcag ttgatcgtcg gtggtaatta
     2281 acagcgacgg acgcgcatct tccagcatca ttttcaggcg atcgtccgga tagccggtat
     2341 ccagcggtag ccaggccgcg ccagcttcaa ctattgcatg gagtgccagg gtcaaaaaga
     2401 ccgagcgcgg tagtgccacc gccacgctgt cccctggttt aacgccgcgc tcacgcagca
     2461 gattcgccag cgccaccacc tgctcgcgca tttcccgata gctgaacagg taacgcgcat
     2521 ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc tgccaccagc gcgctcagcg
     2581 tggtttctgg aatctcaacc tgagtggcgt tgatctgcgc cagctgcgca tactcacctg
     2641 gcagcataat atcgacatcg ccgcacaaca gcgccggatc cgcggcgaac tgggcaatca
     2701 gcattttcag gcgttcagca tgctggatta acgttggctc atcgtaacgc tgtttattgg
     2761 cgaggatctc aatactcaaa tcaccgtgtt catccgggaa cagggccagt tcaaggtcat
     2821 taaccggacc ggttgccagg gtatgggttt gcgtctgaac accaggaata tccagttggt
     2881 aatcaaatac cttgatattg agtaccggac cgaacagcgg ttcctctcct gccgctcgcc
     2941 cgctgtcacg gacaatttgt tcggcatcgt aacgttgatg acgacgcatt tttttcagtt
     3001 gtgctgccag tcgggttgcc agttccggca gcgtttcttg cgccgcaatg tgaataccca
     3061 acggtaaaac gttgagcacg ggtccgctag ccgtcagcgc cgccgagccc agtcgacgca
     3121 taaagataaa tccggcggca tagtccatgc gattgcacaa tcgccccagc cacaaggctg
     3181 ccagcgcaag ggctaaatcg gtacgctgca cacctgaaag ttgcgtagcc agctggcgga
     3241 attccccgtc ggtaaattcc agtttcaggc gcagaatatc tgccgaagcg ctgcgccccg
     3301 ctaaaggtgc cggagaaagt gacgcgggcg gcggcagttg acgacgctgt tctgcccaga
     3361 atgccgcatc acgctgccag gcttcgcttt cgcggtattg ctggtactct tccactacat
     3421 cagcgaaagg cgtaaacggc gaagcaggcg ttggttcgcc acgcagccat gtgcagtaaa
     3481 tattggcgat ctggcgagta atggccggga aactgaagcc atcgaccagc aaatggtgat
     3541 aacgctgata ccagtaccag cggttatccg caacatgtat cagctgatga aagaccagtg
     3601 gtttaccgct atcgacgcgc aaatcttgtt gcaaatccgc cagcattaat gcctgcgcag
     3661 taccgtgcgg atcaatattg gttcgcaggt cgataatttc tggcagttcg aatatcagcg
     3721 catcatcgac ccactgccag acttcgccgt tatcttccgt aaaacgcatc cgcaacgtat
     3781 ctgcttgcgc tagtccgaca accaccgcgc gggccagtaa tggcgcatca acatctccag
     3841 ttaactcaac gtaatgcgcc acgctccagg cggagggtaa atccgacagt ttttctgcca
     3901 tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg gctcattgtg cctcctgcaa
     3961 ctgggtgaaa ttggccggtg tcagagtacg ccagtggact tccagccact gctggcagga
     4021 cgcctgtgac tgcggctgac acacaatgtc ccagcctgcc ggtaaggcgc attgttgcgg
     4081 ccacagactg aattgcccct gcgcattgcg caaaatgtaa aacgctccct gtggatcatc
     4141 gaagggatta ctgaatgcca tattcaactc ctgtcatgga aagtggttgc cagaggtcga
     4201 ttagcccctg catcaagcca ccgcgccaac aaagcgcatc atgtccgccg tcaacctgac
     4261 gccagaaaat ggattctttt atggggtgta attgcgcata cagcgcctga ttggcccgca
     4321 taatcatcgg ctcgcgaata cccgcttcca gcacaatgcg cagaccttcg gcgctaactt
     4381 caccagcttt aagcttttca agtaacacgc cctcttgctg cccgccccga tgcggccacc
     4441 agtacgatcc tgactggctt aatacacagc caaagcgttc aggccagtgc agtccggcat
     4501 acagcgcgga cagcccacca aaactctgcc cggcgaccac ggtgcgatcg gcgcgatcgc
     4561 taaaaggggc aatagctttc accaggggta ataactcttg ctgtactgcc agccagaaat
     4621 ccgcattaca cggcagttcg tgggcgcggt gcgtggtgtc gatagcgtcg atcaacacat
     4681 acacggcggg aggaagttgc tgacgatggg tcagcgaagt cagcacaggc cagacgggca
     4741 tactttgcgc ccaaaattcg ccatcgagca aaactgccag cggacgttct tctgctgttg
     4801 catcgccggt ggtaaaaatc catacacgac gtgaattttt caaccgttca cttttccaga
     4861 taatttcttt ggctgctatt tctggcgctt gcggacaatc ccatcccggt tgcagaggcg
     4921 cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc gcctttccag ctttgtggat
     4981 tcagcggatc ggctattgcc tggggtaata gttttcgcca gccttcacgt aattcgaggc
     5041 gatcggggga tggtgcagaa aaaatgtcat cgcgttcggt gggaataaag cagtagctgc
     5101 cgcgccagtt ggcattgagt tgtgtcgtcc actgccagac atcagtacct gcaattcgct
     5161 gcatcgactg gggctggctg ttctgatgat gatcggtcac accagtgatg tagatccata
     5221 cgcgctttat cgtcgagtat tcttcagaac cttggggatc acgccaccag aaagtgacct
     5281 caaacatttc gtcattcaga cgctgccatt ccgggccatg tttcgactgc caccagctct
     5341 cacttcctac ttttaacgcc gtcacaccat aaccccatgt ttactgtgca atttttcatt
     5401 gattgcagaa atatattgat aatattattg ataactattt gcatttgcaa tagcgtaatg
     5461 gcgcgccgtg ggaagcgcgg acatttatta accaactgca ctgcgtgtct ttcaggatca
     5521 aaggttttcg cggtagcggg atgcgtcgtg ttgaggacga ccatgcccga cagttgcaat
     5581 tcgtggcaaa aatgcaggaa taaaacaatg aacaagaaga ttcattccct ggccttgttg
     5641 gtcaatctgg ggatttatgg ggtagcgcag gcacaagagc cgaccgatac tcctgtttca
     5701 catgacgata ccattgtcgt taccgccgcc gagcagaact tacaggcgcc tggcgtttcg
     5761 accatcaccg cagatgaaat ccgcaaaaac cccgttgccc gcgatgtgtc ggagatcatc
     5821 cgtaccatgc caggcgttaa cctgaccggt aactccacca gtggtcagcg tgggaataac
     5881 cgacagattg atattcgcgg tatgggtccg gaaaacacgc tgattttgat tgacggcaag
     5941 ccggtaagca gccgtaactc ggtgcgtcag ggctggcgtg gcgagcgcga tacccgtggt
     6001 gatacctcct gggtgccacc tgaaatgatt gaacgtattg aagttctgcg tggtccggca
     6061 gctgcgcgtt atggcaacgg cgcggcgggc ggcgtggtta acatcattac caaaaaaggc
     6121 agcggtgagt ggcacggctc ctgggacgct tatttcaatg caccagaaca taaagaggaa
     6181 ggagccacta aacgcaccaa cttcagcctg accggtccgc tgggcgacga attcagcttc
     6241 cgcttgtatg gcaacctcga caaaacccag gctgacgcgt gggatatcaa ccagggccat
     6301 cagtccgcgc gtgccggaac gtatgccacg acgttaccag ccgggcgcga aggggtgatc
     6361 aataaagata ttaacggcgt ggtgcgctgg gacttcgcac cattgcaatc gctggaactg
     6421 gaagcgggtt acagccgcca gggtaacctg tatgcgggcg atacccagaa caccaactct
     6481 gacgcttaca ctcgctcgaa atatggcgat gaaaccaacc gcctgtatcg ccagaactac
     6541 gcgctgacct ggaacggtgg ctgggataac ggcgtgacca ccagcaactg ggtgcagtat
     6601 gaacacaccc gtaactcgcg tattccggaa ggtctggcgg gcggtaccga agggaaattt
     6661 aacgaaaaag cgacacagga tttcgtcgat atcgatctcg atgacgtgat gctgcacagc
     6721 gaagttaacc tgccgattga tatcctcgtt aaccagacgc tgacgctggg tacggagtgg
     6781 aatcagcaac ggatgaagga cttgagttcc aatacccagg cgctgaccgg ggcgaacacc
     6841 ggcggcgcta ttgatggtat gagtgccacc gaccgtagcc cgtattcaaa agcagaaatt
     6901 ttctcgctgt ttgccgaaaa caacatggag ctgactgaca gcaccatcgt aacgccgggg
     6961 ctgcgtttcg atcatcacag tattgtcggc aataactgga gcccggcgct gaacatatcg
     7021 caaggtttag gcgatgactt cacgctcaaa atgggcattg cccgagctta taaagcaccg
     7081 agcctgtacc agactaaccc gaactacatt ctctacagta aaggtcaggg ctgctatgcc
     7141 agcgcgggcg gctgctatct gcaaggtaac gatgacctga aagcagaaac cagcatcaac
     7201 aaagagattg gtctggagtt caaacgcgac ggctggctgg cgggcgtcac ctggttccgt
     7261 aacgattatc gcaataagat tgaagcgggc tatgtggctg tagggcaaaa cgcagtcggc
     7321 accgatctct atcagtggga taacgtgccg aaagcagtgg ttgaaggtct ggaaggatcg
     7381 ttaaacgtac cagttagcga aacggtgatg tggaccaata acatcactta tatgctgaag
     7441 agtgaaaaca aaaccacggg cgaccgtttg tcgatcatcc cggagtacac gttgaactca
     7501 acgctgagct ggcaggcgcg ggaagatttg tcgatgcaaa cgaccttcac ctggtacggt
     7561 aagcagcagc cgaaaaaata caactacaaa ggtcagccag cggttggccc ggaaaccaaa
     7621 gaaatcagtc cttacagcat tgtgggtctg agcgcgacct gggatgtgac gaagaatgtc
     7681 agtctgaccg gcggcgtgga caacctgttc gacaaacgtt tgtggcgtgc gggtaatgcc
     7741 cagaccacgg gcgatctggc aggggccaac tatatcgcgg gtgccggggc gtatacctat
     7801 aacgagccgg gacgtacgtg gtacatgagc gtaaacactc acttctgatg ctaacgtcag
     7861 attgttgaca aagtgcgcgt cgttcatgcc ggatgcggcg tgaacgcctt atccggccta
     7921 caaaaatctt gccaattcaa tatattgcag gaccatgtag gcctgataag cgtagcgcat
     7981 caggcagttt agcgtttgtc atcagtctct aatatggtcg acatgaaaac tacgcatacc
     8041 tccctcccct ttgccggaca tacgctgcat tttgttgagt tcgatccggc gaatttttgt
     8101 gagcaggatt tactctggct gccgcattac gcgcaactgc aacacgctgg acggaagcgc
     8161 aaagccgaac atttagccgg gcggatcgct gcaatttatg cgctgcggga atatggctat
     8221 aaatgtgtgc ccgcaatcgg cgagctacgc caaccggtgt ggcctgcgga ggtatacggc
     8281 agtattagcc actgcggggc tacggcattg gccgtggttt cccgtcaacc gattggcgtt
     8341 gatatcgaag aaattttttc tgcgcaaaca gcgaccgaat tgacagacaa cattattaca
     8401 ccagcagaac acgagcgact cgcagactgc ggtttagcct tttctctggc gctgacactg
     8461 gcattttccg ccaaagagag cgcatttaag gcaagtgaga tccaaactga tgcaggtttt
     8521 ctggactatc agataattag ctggaataaa cagcaggtca tcattcatcg tgagaatgag
     8581 atgtttgctg tgcactggca gataaaagaa aaaatagtca taactctgtg ccaacacgat
     8641 taattgacaa catctggtac gattcgcccg cagccatcac tgaccgcggg cgaaagtgta
     8701 aagcaggtgc cttaccactc tgacctgaca accggatatg cggggattgc tccccgcaca
     8761 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttta
     8821 cggtgaactc acacagcgag tctccgacca gaagcgtaaa tcccagtacc gtcaaacaca
     8881 gcactattac tgccacaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
     8941 aagggactac catccgagtt gttgaggctc ggagtggcag ccccaggatt gatagaaata
     9001 tctcctgggg cttcatcttt ccagacctca gagttaacct gaaaccggaa agcttcaggc
     9061 acccgccgtt atcttaccct tcctcaacaa aatatctgtt tatttataca gtgaatcctg
     9121 tttcggactt ctgtcggcaa aactcatcat tgctcttaaa acgaaatcgc ccgcagccat
     9181 cactgaccgc gggcgaaagt gtaaagcagg tgccttacca t
//
LOCUS       DAZFDP010000172         9196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFDP010000172 DAZFDP010000000
VERSION     DAZFDP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..865
                     /locus_tag="ACHJ1G_004294"
     CDS             <1..865
                     /locus_tag="ACHJ1G_004294"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4766044.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
     gene            875..1207
                     /locus_tag="ACHJ1G_004295"
     CDS             875..1207
                     /locus_tag="ACHJ1G_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4766045.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            1263..2288
                     /locus_tag="ACHJ1G_004296"
     CDS             1263..2288
                     /locus_tag="ACHJ1G_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4766046.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            2330..2725
                     /locus_tag="ACHJ1G_004297"
     CDS             2330..2725
                     /locus_tag="ACHJ1G_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4766047.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            2737..3111
                     /locus_tag="ACHJ1G_004298"
     CDS             2737..3111
                     /locus_tag="ACHJ1G_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4766048.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            3102..3680
                     /locus_tag="ACHJ1G_004299"
     CDS             3102..3680
                     /locus_tag="ACHJ1G_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766049.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            3677..4072
                     /locus_tag="ACHJ1G_004300"
     CDS             3677..4072
                     /locus_tag="ACHJ1G_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4766050.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            4080..4820
                     /locus_tag="ACHJ1G_004301"
     CDS             4080..4820
                     /locus_tag="ACHJ1G_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766051.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            4836..5258
                     /locus_tag="ACHJ1G_004302"
     CDS             4836..5258
                     /locus_tag="ACHJ1G_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4766052.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            5240..5674
                     /locus_tag="ACHJ1G_004303"
     CDS             5240..5674
                     /locus_tag="ACHJ1G_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4766053.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            5667..8228
                     /locus_tag="ACHJ1G_004304"
     CDS             5667..8228
                     /locus_tag="ACHJ1G_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766054.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYKRLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            8225..8554
                     /locus_tag="ACHJ1G_004305"
     CDS             8225..8554
                     /locus_tag="ACHJ1G_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766055.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            8554..>9196
                     /locus_tag="ACHJ1G_004306"
     CDS             8554..>9196
                     /locus_tag="ACHJ1G_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4766056.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
BASE COUNT         2092 a         2354 c         2937 g         1813 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
      421 catgcgtgat gcactggatg cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga
      481 gactcaatca acaactgttt cagccactgc ttcgcaggct gacgttactg acgtggtgcc
      541 agcgacggag ggcgaaaacg ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc
      601 agcggttgcg gcagaaaaca gccgcattat ggggatcctc aactgtgagg aggctcacgg
      661 acgcgaagaa caggcacgcg tgctggcaga aacccccggt atgaccgtgg aaacggcccg
      721 ccgcattctg gccgcagcac cacagagtgc acaggcgcgc agtgacactg cgctggatcg
      781 tctgatgcag ggtgccccgg caccgctggc tgcaggtaac ccggcatctg atgccgttaa
      841 cgatttgctg aacacaccag tgtaagggat gtttatgacg agcaaagaaa cctttaccca
      901 ttaccagccg ctgggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag
      961 tgcgaaagcg cctgcaatga ccccgctgat gctggacacc gccacccgta agctggttgc
     1021 gtgggatggc accaccgacg gtgctgccgt tggcattctg gcggttgctg ctgaccagac
     1081 cagcaccacg ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc
     1141 ggaggctgcc agcgatgaga cgaaaaaacg gaccgcgttt gccggaacgg caatcagcat
     1201 cgtttaacct tacccttcat cactaaaggc cgcctgtgcg gcttttttta cgggattttt
     1261 ttatgtcgat gtacacaacc gcccagctgc tggcggcaaa tgagcagaaa tttaagtttg
     1321 atccgctgtt tctgcgtctc tttttccgtg agagctatcc cttcaccacg gagaaagtct
     1381 atctctcaca aattccggga ctggtaaaca tggcgctgta cgtttcgccg attgtttccg
     1441 gtgaggttat ccgctcccgt ggcggctcca cctctgaatt tacgccggga tatgtcaaac
     1501 ccaagcatga agtgaatccg cagatgaccc tgcgtcgcct gccggatgaa gatccacaga
     1561 atctggcgga cccggcttac cgccgccgtc gcatcatcct gcagaacatg cgagacgaag
     1621 agctggccat tgctcaggtc gaagagatgc aggcagtttc tgccgtgctt aagggcaaat
     1681 acaccatgac cggtgaagcc ttcgatccgg ttgaagtgga tatgggccgc agtgtggcga
     1741 acaacatcac gcagtccggc ggtacggagt ggagcaagcg tgacaagtcc acgtatgacc
     1801 cgaccgacga tatcgaagcc tacgcgctga acgccagcgg tgtggtgaat atcatcgtgt
     1861 ttgatccgaa aggctgggcg ctgttccgtt ccttcaaagc cgtcaaggag aagctggata
     1921 cccgtcgcgg ctctcattcc gagctggaga cagcggtaaa agacctgggc gaagcggtgt
     1981 cctataaggg gatgtatggc gatacggcga tcgtcgtgta ttccggacag tacgtggaaa
     2041 acgacgtcaa aaagaacttc cttccggaca acacgatggt gctggggaac actcaggcac
     2101 gcggtctgcg cacctatggc tgcattcagg atgcggacgc acagcgcgaa ggtattaacg
     2161 cctctgcccg ctacccgaaa aactgggtga ccaccggcga tccggcgcgt gagttcacca
     2221 tgattcagtc agcaccgctg atgctgctgg ctgaccctga tgcgttcgtg tccgtacaac
     2281 tggcgtaatc atggcccttc ggggccattt tctctctgtg gaggagtcca tgacgaaaga
     2341 tgaactgatt gcccgtcttc aggtgctggg tgagcaactg aaccgtgatg tcagcctgac
     2401 ggggacgaaa gaagaactgg cactccgtgt ggcagagctg gaagaggagc ttgatgacac
     2461 ggatgacgct gccggtcagg acacatctgt cagcccggaa aatgcgctga ccggacatga
     2521 aaatgaggtt gtatcagcgc agccggatac cgtgactgat acggctgatc tggtcacggt
     2581 tgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggatg aggctgtggc
     2641 atttgtgctg ccgggaacgg cgttccgtgt ctctgccggt gtggcagctg aaatgacaga
     2701 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     2761 gatgctgcca ttgcctgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     2821 atgacatccg gtgagcagtc cggtgctgtg atacgtggtg tttttgatga ccctgaaaat
     2881 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     2941 actgatgatg tgcggcagct gcggcgcggc gacacgctga ccatcggtga ggaaaacttc
     3001 tggatagacc ggatttcgcc ggatgatggc ggaagctgtc atctctggct tgggcggggc
     3061 gtaccgcctg ccgttaaccg tcgccgctgg aagggggatg tatggccata aaaggtcttg
     3121 agcaggccgt tgaaaacctc agccgtatca gcagaacggc ggtgcccggt gccgccgcaa
     3181 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc ggtgtcacag gttgcccgtg
     3241 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     3301 aaaatccgca ggccagaatc aaggttaacc ggggggattt gcccgtaatc aagctgggta
     3361 atgcgcgggt tgtcctttcg cgccgcaggc gtcgtaaaaa ggggcagcgt tcatccctga
     3421 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     3481 aactgaaaaa tggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     3541 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa caaaatattg
     3601 agcggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     3661 ggatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     3721 gaagcatgac accggggcga cgttttttga tggtcgcccc gctgtttttg atgaggcgga
     3781 ttttccggca gttgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     3841 cgatacctgg caggcggagc tgcatatcga agttttcctg cctgctcagg tgccggattc
     3901 agagctggat gcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     3961 gtcagagttg atcaccagta tggtggccag tggctatgac taccgacgcg acgatgatgc
     4021 gggcctgtgg agttcagccg atctgactta tgtcattacc tatgaaatgt gaggacgcta
     4081 tgcctgtacc aaatcctgta atgccggtga aaggtgccgg gaccaccctg tgggtttata
     4141 aggggagcgg tgacccttat gcgaatccgc tttcagacgt tgactggtcg cgtctggcaa
     4201 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
     4261 atgatgaaga tgcggactgg actgcgaccg ggcaggggca gaaatcagcc ggagatacca
     4321 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
     4381 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
     4441 gtggctgggt cagcagtatc ggtaaagcgg tgacggcgaa ggaagtgatc acccgcacgg
     4501 tgaaggtcac caatgtggga cgcccgtcga tggcagaaga tcgcagcacg gtcacagcgg
     4561 caaccggcat gaccgtgacg cctgccagca cctcggtggt gaaagggcag agcaccacgc
     4621 tgaccgtggc attccagccg gaaggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
     4681 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
     4741 caggcaaggt caacattccg gtcgtatccg gtaatggtga acttgctgcg gttgcagaaa
     4801 tcactgtcac cgacagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
     4861 aacataacgg cgtgaccgtc acgctttctg aactttcagc cctgcagcgt attgagcatc
     4921 tcgccctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttacag
     4981 tggaagacgc catcagaacc ggtgcttttg tggtggcgat gtccctgtgg cataaccatc
     5041 cgcagaagac aaagcagcct tccatgaatg aagccgttaa acagattgag caggaagtgc
     5101 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
     5161 gtatgtatga gtttgtggtg aatgatgccc ctgaacaggc agaggacgcc gggcctgcag
     5221 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
     5281 cgagatgggg cgacccgact ggcgtgccat gcttgccggg atgtcatcca cggagtatgc
     5341 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
     5401 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggata tgcatccgct
     5461 ggatttcagt ctgctgaacc ggcgtgaggc tgacgaagag cctgaagatg atgtgctgat
     5521 gcagaaagcg gcagggcttg ccggaggcgt ccgctttggc ccggacggga atgaagttat
     5581 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
     5641 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
     5701 taagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
     5761 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
     5821 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
     5881 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg cgggcaaagt ccgtggctga
     5941 tcctgctgca acaggggggg caggtgaagg actccttcgg cgggatgatc cccatgttca
     6001 gggggcttgc cggtgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
     6061 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
     6121 cgctggtcct ttccggcaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
     6181 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
     6241 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
     6301 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
     6361 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
     6421 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
     6481 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
     6541 gcacgctgga gacctgggca gacaggactg cgcgggcctt caaatccatg tgggatgcgg
     6601 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
     6661 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttattttgtt aacgatgaag
     6721 cgcgggcgcg ttactgggat gatcgtgaaa aggcccgtct tgcgcttgaa gccgcccgaa
     6781 agaaggctga gcagcagact caacaggaca aaaatgcgca gcagcagagc gataccgaag
     6841 cgtcacggct gaaatatacc gaagaggcgc agaaggctta caaacggctg cagacgccgc
     6901 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac gggaaaatcc
     6961 tgcaggcgga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
     7021 aaaagccgaa acagtccggc gtgaaggtgt ctgcgggcga tcgtcaggaa gacagtgctc
     7081 atgctgccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
     7141 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
     7201 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
     7261 aagatgagac gctggagtac aaacgccagc tggctgcact tggcgacaag gttacgtatc
     7321 aggagcgcct gaacgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
     7381 aacgggccgc cattgatgcg aaaagccggg ggctgactga ccggcaggca gaacgggaag
     7441 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc gctggcgctg aataacgtca
     7501 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
     7561 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcgcaggtaa
     7621 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcagcg atgctgaccg
     7681 gcagtgagca gaactggcgc agcttcaccc gttccgtgct gtccatgatg acagaaattc
     7741 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
     7801 ttggtggcgg cgcatccgcg tcaggcggta cagccattca ggccgctgcg gcgaaattcc
     7861 attttgcaac cggaggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
     7921 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
     7981 accggctgat gcgcggctat gccaccggtg gttatgtcgg tacaccgggc agtctggctg
     8041 acagccggtc gcaggcgtcc gggacgtttg agcagaataa ccatgtggtg attaacaacg
     8101 acggcacgaa cgggcagata ggtccggctg ctctgaaggc ggtgtatgac atggcccgca
     8161 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
     8221 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
     8281 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
     8341 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca cggtactgga
     8401 gtcgtttctg gaagagcacg ggggctggaa atcctttctg tggacgccgc cttatgagtg
     8461 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
     8521 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
     8581 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgacctgaca
     8641 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
     8701 acctggcagg ggcgacagta tcaggcgtat cccattcagg ggacgggatt tgaactgaac
     8761 ggcaagggca gtgctgcccg tccgacactg acggtttcta acctgcacgg catggtcacc
     8821 ggtatggtgg aagatatgca gagtctggtc ggcggaacgg tggtcaggcg taaggtttac
     8881 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gttacgccga tccggagcag
     8941 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctcc
     9001 tttgtactgt ccacgccgac ggaaacggat ggcgctgttt ttccgggacg tatcatgctg
     9061 gccaacacct gcacctggac ctatcgcggt gacgagtgcg gttatagcgg tccggctgtc
     9121 gcggatgaat atgaccagcc aacgtccgat atcacgaagg ataaatgcag caaatgcctg
     9181 agcggttgta agttcc
//
LOCUS       DAZFDP010000173         9047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFDP010000173 DAZFDP010000000
VERSION     DAZFDP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..827
                     /gene="nanC"
                     /locus_tag="ACHJ1G_004307"
     CDS             111..827
                     /gene="nanC"
                     /locus_tag="ACHJ1G_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4766057.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            847..1953
                     /gene="nanM"
                     /locus_tag="ACHJ1G_004308"
     CDS             847..1953
                     /gene="nanM"
                     /locus_tag="ACHJ1G_004308"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4766058.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2018..2998
                     /gene="nanS"
                     /locus_tag="ACHJ1G_004309"
     CDS             2018..2998
                     /gene="nanS"
                     /locus_tag="ACHJ1G_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4766059.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(3006..3656)
                     /locus_tag="ACHJ1G_004310"
     CDS             complement(3006..3656)
                     /locus_tag="ACHJ1G_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4766060.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3793..3936
                     /locus_tag="ACHJ1G_004311"
                     /pseudo
     CDS             3793..3936
                     /locus_tag="ACHJ1G_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4600..4728)
                     /locus_tag="ACHJ1G_004312"
                     /pseudo
     CDS             complement(4600..4728)
                     /locus_tag="ACHJ1G_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4704..4853)
                     /locus_tag="ACHJ1G_004313"
                     /pseudo
     CDS             complement(4704..4853)
                     /locus_tag="ACHJ1G_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4938..5135)
                     /locus_tag="ACHJ1G_004314"
     CDS             complement(4938..5135)
                     /locus_tag="ACHJ1G_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4766061.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5147..5635)
                     /locus_tag="ACHJ1G_004315"
     CDS             complement(5147..5635)
                     /locus_tag="ACHJ1G_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4766062.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(5632..6006)
                     /locus_tag="ACHJ1G_004316"
     CDS             complement(5632..6006)
                     /locus_tag="ACHJ1G_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4766063.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(6096..6464)
                     /locus_tag="ACHJ1G_004317"
     CDS             complement(6096..6464)
                     /locus_tag="ACHJ1G_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4766064.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(6627..6848)
                     /locus_tag="ACHJ1G_004318"
     CDS             complement(6627..6848)
                     /locus_tag="ACHJ1G_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4766065.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6911..7387)
                     /locus_tag="ACHJ1G_004319"
     CDS             complement(6911..7387)
                     /locus_tag="ACHJ1G_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4766066.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(7403..7888)
                     /locus_tag="ACHJ1G_004320"
     CDS             complement(7403..7888)
                     /locus_tag="ACHJ1G_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4766067.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7943..8761)
                     /locus_tag="ACHJ1G_004321"
     CDS             complement(7943..8761)
                     /locus_tag="ACHJ1G_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4766068.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            complement(8861..>9047)
                     /locus_tag="ACHJ1G_004322"
     CDS             complement(8861..>9047)
                     /locus_tag="ACHJ1G_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4766069.1"
                     /translation="GQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRRWRCRNSEPD
                     AGKVLNTRLASDGLLRQ"
BASE COUNT         2368 a         2130 c         2305 g         2244 t
ORIGIN      
        1 cggttgccaa agacactata agcctggcaa aaaaatatta cacaacataa atgctaattg
       61 tttatgcggg ctttgtattg ctttctgttt cctacaaatg agtgaaattt atgaaaaagg
      121 ctaaaatact ttctggcgta ttattactgt gcttttcgtc accattaatt tctcaagctg
      181 cgacactgga cgtacgtggt ggatatcgta gtggaagcca cgcctatgag actcgactca
      241 aagtcagtga gggatggcaa aatggatggt gggcaagtat ggaaagtaat acctggaata
      301 ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt gatgttaatt
      361 acgcgattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg cattttagca
      421 gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca aaagatctta
      481 attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac ttatccggtg
      541 atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat attaatagtg
      601 atttcacctt cgcatggcaa acgacgctat acagcaaaca gaacgattat cgctatgcaa
      661 accataagaa atgggcgacg gaaaatgcat ttgttctaca ataccatatg acgccggata
      721 ttacgccata catagaatat gactaccttg accgtcaggg tgtttacaac ggcagagata
      781 atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag acaggagaca
      841 gtcacaatga ataaaacaat aacggcgctt gctatcatga tggcttcatt tgccgcaaac
      901 gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg gtaccggagc aattgataac
      961 gacactgtct acattggttt aggtagcgca ggtacggcat ggtacaagct ggatacacag
     1021 gccaaagata aaaaatggaa agcgttagct gcattccctg gtggaccaag agatcaagca
     1081 acctcggcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa aaacagcgag
     1141 ggcttgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagttgggtt
     1201 aaattgatgt cgcacgcacc gatgggcatg gcgggtcatg taacttttgt acacaacggc
     1261 aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc
     1321 aacgaggctg gaaaagattc aaccgctata gataaaatca acgctcacta ttttgacaaa
     1381 aaagcagaag attatttttt caataagttt ctgttgtctt ttgatccctc aacacagcaa
     1441 tggagttacg ctggcgaatc gccctggtac ggaacggctg gtgcggcggt tgtgaataaa
     1501 ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta
     1561 tttgaacttg atttcaccgg taataattta aaatggaata agcttgctcc cgtctcatca
     1621 ccagatggcg tagctggcgg ttttgcgggg ataagcaatg attctcttat atttgccgga
     1681 ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa
     1741 ggcctgaaaa aatcatatag cactgatatt catctttggc ataacgggaa atgggataaa
     1801 tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagtcta
     1861 ttgattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt tttgatctct
     1921 gtgaaggata ataaagttac agtacaaaac taacgcttca gggccccggt aaaggggccc
     1981 tgctatcaat ttgtcatttc aattacgcga aatttatatg aacgcaataa tatcgcccga
     2041 ttattactat attcttacca ttgctggtca gtctaatgcc atggcgtatg gcgaaggact
     2101 gccattaccg gacagggaag atgcgcctca tcccagaatc aaacaattag cgagatttgc
     2161 gcatacgcat cccggaggcc cgccatgtca ctttaacgac atgattccac tgactcactg
     2221 cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac
     2281 acagtacggc accgttggcc aggcactgca tatagcacgg aaattactgc cctttattcc
     2341 tgataatgca ggggttctca tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg
     2401 cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc gttggggaac
     2461 ggatactccg ctataccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc
     2521 gcataacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga
     2581 ctacgcgtca caccctcaac actttaatca tatggttgaa gcctttcgta gggatctaaa
     2641 acaataccat tctcagctta ataatattac tgacgcaccg tggttttgcg gcgataccac
     2701 ctggtactgg aaagaaaatt tccctcatgc gtatgaagtt atttatggca attatcaaaa
     2761 taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac
     2821 gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg
     2881 gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcacgg cagcccgtcg
     2941 ggggattatt tctgacaagt ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc
     3001 gttcattaat ttgatttatt cgcatccatc agcgctttta actgctccgg cgtaaacggt
     3061 ggtttgcgtc ggtgcaacat cgcatggcaa ttagggtaca caggcagcaa atcattaact
     3121 ggatccagct ggtaatcctc tttaatggct gacagcggca ccaggtgatg cacatggata
     3181 aagccttcag cgatatcacc atagatcttc accagatcaa caccgcatac gttgcactgg
     3241 catccatgat gttcgaccgc tttatctcgg gcctttttat cgcgttcata gcggtttact
     3301 gtcaccttta tcgccgcacc ttcaaggtat gtctcctgcg acggcaactc atccgggaac
     3361 agaacgtcgg ctatgggagc cgcagcggct ttatccagat gaatatgaaa tccctgctct
     3421 atgagtagct tgacacattt agattttatt ccgccggtga aatccgcagg ggtaaattgc
     3481 gtccccgtca tcaatgtcgc tgcgatgcca gcaatcgcct tcgaggggta acgtcggcca
     3541 tgatattcaa tctcatacaa ccgcgcaggc tggaactgat gagtttctcc cgcatcccat
     3601 aaatggatag cttcgatgat ataccgccgc ggaagatgat cagggagttt attcatggag
     3661 aatgtcctta tacgttctca gaacaggcag caaaaaaaca aatgagatca aggtaatata
     3721 tcttcacttg caggcttgaa cagcagaaaa tttgcgagca tgatcgcaat tatttcactg
     3781 ccagggatat caatagcttc cagtaaatcg ctacagcagg caattgccaa tataaaaatc
     3841 tggcacaagg gtgaacagcg cgcgccgcat aagccattgt tattgctata cgtattagcg
     3901 ggatacctga atggacatcc gcgccttttc gattattgta gtggaacagt ctacgaggat
     3961 gcagttcgaa gtgagtaagt aaaggttgcg ggaaacgatc aatgccgcca tacaatgatt
     4021 ttttaggatc aacaatgagt aataaaaact acgaaagtca ccgcaaagcg attgttagta
     4081 aaggtatacc acccgctcta ttaaataggc tcaccaattc ggatgttcag gtgatcaata
     4141 ccttcttaac acgagtgagc aaactggaac tatctcaaca agagaaagac tggatcataa
     4201 aaatcatctc tatggtttag acaatttcag ccttgtagcc tttcaagggt tttgtgtggg
     4261 tacaaacatt atcgaatcgg ggtatagccg cagctgtagc tgtatccaca tcagtaagtt
     4321 gatgtaggtt gacttaggtt gagcgaaaaa tagcgaaaag ccttgtgtgg cgcggatttc
     4381 agtcataaaa aaacgtccgt tgatgtctat tgatgttccg atggtgcgaa ggtgcgaagg
     4441 tgcgaaggcc ggactcaaac atgtgactta acctcatgat ttaaaattgt taataataaa
     4501 caatatcatc ttataccaac acagatacca acacgaaaaa tcactggttt tcggcatcga
     4561 accaggacaa tcagctcctg attgagatac cgagctctgt tactcgtcat cttcctcgac
     4621 aggcttcatt gcatcaagta gtactcgcac catcgcgata gaaccaatcc ggataaaagc
     4681 atcttctcca tctatatctg ttaactgata tcgctcactt gatgccggat aattgtgtcg
     4741 atttgcagca gtgaactctt tcgtacagcc agaatgcggc ggatgctggt tgagcagata
     4801 acgtccgtat tctcgttgac cacttccgga cggtaggctg tgatatcaga cataatcgat
     4861 attccttttt gcagataaat gaaaatgcca gccctgttca ggctggcgtc agtggaatga
     4921 agtctggtga gtaagactca ccggttctgg tgctgaagct gcagttggcg cagggtacgg
     4981 atatcctctc tggcctgttc gatacagggc aacagtgctg ctgaatccgt tttatcctca
     5041 ttgttgtcaa agataattcc cgattcgcag tcgatattgt cctgcagcca cgcaatcaga
     5101 atatccagtg cggtttccgt ggttaatgat ttcatgttgt tcctggttac cagttgaaag
     5161 tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac
     5221 gtcgggtaag tgtgataacc agacgacgga acgtttttga cagccccagg cgtttaagcc
     5281 gcagaaccgg atatgaccac gcatcggtac gtaacagata tccgctacct gtgaaatgaa
     5341 tccattctga ttcaccaacg tcactggtct gatgcgacag cgagtacagc caggcgttat
     5401 ccttttccgt gatatgtgcg gtactgcagc gtatgccggc aagggttgca aacggtggta
     5461 gtgtgcaggt tgactgttgg tcggattcct ccaccacgcg gagtgaataa ccgttttcag
     5521 cgaccttgtt aatcagttca gtgagattaa caccatcgac gtcaacgaca attcgcccca
     5581 tgttcagtgc ctgcacatta attctgtcgg cttccagcat cagggaaagt ttcatcgttt
     5641 ctccggatgc ttacccaggg taatgttatt taccgttctg taattgtcgc gggtcatcag
     5701 gccggtcgcc ctgcgagccc ggaggatatc aatactgttt attaactgag agcggggaca
     5761 agtgctgaat cccggctggt cggtacgcac cagcgcgtat ttttccacga gaaaattcac
     5821 cgcatcacac agtgaaatgc cagcttcaat atgctgctca atcacacgtt catcggcgaa
     5881 cggtgtgtca ttcagtgtaa ggccgtaatg ctggcccagt agtcgggaga gcagagtctg
     5941 ccagatggtg acgggagacg ggcagctcga tgcctcccgt acgtgtgtat cgggtaatgt
     6001 tttcatactg aaggttttcc tgatatgcag agatgaaaaa tggaaaagtg gcgtggtgaa
     6061 agcaccaggc cggagcagaa ggttattccg gtggtttact ttttcgtttc gggtgtcgga
     6121 taaacagcca gataaacgta accaccgcta ccgagagtgt cagctttgca ggtcagacct
     6181 tttgcataca gcgtgacggt atgctgatgg cggggattca gttcaccgct ggtgagcatg
     6241 agttccagtt gtttcatcag cagcggaaag gcctggtcca ggtggtacgc atctgcatcg
     6301 ctgaacaggc ctctgatacc ggcgcggtcg gcaaggtaat gcaggcggtt accctcctgc
     6361 accagacggg cgccgaaaca gggcgtcact gtgcagggta gtccccacca gggatggtcg
     6421 tcattgtcat cgggatgcgt tgtcccggaa agtgtgtctg acacgataaa atcctcacag
     6481 aaaatcggtg aatgatagtt taacgatgac gggtcagccg gcgcgcagac cggcttcggt
     6541 aaaggtgtat tcgttgatga gcaggacctc atccacagcg gcgtctgagc tcagccagtc
     6601 gtactcgttt tccgggtgac ggaacatcag ttcagggtga cgctcatcag ccggacatac
     6661 ggaccaaaac tgtccttacg gcgttctgca aacacggcca gcacaccggg aatatcctgc
     6721 acttcacgac cggtatacgc ttcagcactg ccgtgccagc ggtatttgcc ggtacagaac
     6781 ggaaaaagac gggatgccgg atgctgacgg tgaatacgca tggcttcgcc acgggtgatg
     6841 attttcatag tgggatacct ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg
     6901 ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggctg ccaccgacta
     6961 tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga cgttccgtga
     7021 taaggcggtc tgccttgctg ggtgtgactt caccggacgg gtgattgtgt gccagcacca
     7081 cagcagcggc attgtgatac agggcgcgtt taatcacttc acggggatgg acttccgtgc
     7141 ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat
     7201 acagcacccg gaactcttca cgctccagtc ccgccatgtt cagaatcagc cattctcgtg
     7261 ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca
     7321 gggcccgttg aatgaggctg cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct
     7381 gttgcattgt acttctctcc attcagtcga tgatgcgcat aatggcgctg cattccggat
     7441 gctgcagggc gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac
     7501 gacaggcatg atgactgtac gtcatcagac aggcagcaat gccggcagct tccggactca
     7561 tttcagcgcg gttaccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat
     7621 ccggttcggg tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac
     7681 cgcagtagtc ttcacacaga cggtccatcc agccgaagac acggggctcc agggtcaccc
     7741 actgtggaat gaggccaaag tgctgcggcc agaagctgat gcgctgttca tcagggacga
     7801 gggtggcaac caactgaggc tggtcattcc ctgatgcagc ggttacggaa acagaagatg
     7861 tggtggaatt atgtgaaatg gttttcatga aatgactcct taaaggacaa ataaatgcgt
     7921 ggttttcagg gtaatggctg aatcagcgca ggctctccag cagggtttcc gccatcaccc
     7981 acaatacgcg gttgagctta atgtcagtgt caatgctgtg aatggcacgg gtatggatac
     8041 gttttccttt agcgctgcga cctgaaattc cgcctttcag catattctcc tgaatggtct
     8101 gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggcgtc agaatgtcgg
     8161 cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc tgtgccagcg
     8221 cctggcgtgc cggtggcggc agaatcagcg actgcatggc atcacgcttt tcctcaatcc
     8281 ggtcaaacac gcccaccacc tcgtaagccc cttcaatgac tctgtccacc acatttcccc
     8341 ggtgcggaac acgcacttcc cccagagact gaccacagac gcatccgttc tggcagacga
     8401 acctgaagta acccggcagc atctggtagc tggaggtacc gtcatgagag ttgagcagaa
     8461 taatttcagg gacatgttct ccgtttatct ctccggcccg ccgcagacgc agcatgtgtt
     8521 ttgtgtatcc ccggcggccc gggtcgcgca cacgggtctg gcaggcgaag aatggctgaa
     8581 agccttcccg ctgcaggctt tccagtacgg tgatggtggg gatgtacgta tagttctggc
     8641 ttcgggacgt gtgtttatct tccccgaaaa tccccggcac atagtgcatc agttcttcgt
     8701 gtgtcagcgg acggtcacgg cgtatctggt tcgcataacc aaaacgactg gctagtcgca
     8761 taatttgctc cttatcggtg gttaagattt actggtgtaa taaatgaaaa agccacgtct
     8821 cccggagaag acgcggcctg atgggtgaaa tgaatgacgt ttattgtctg agaagaccgt
     8881 cactggcgag ccgcgtatta agcacctttc cggcatcggg ttcagaattc cggcagcgcc
     8941 agcgtcgctg accttccgcg ttgcggataa cccgacggaa atgcattccc ggatcatcct
     9001 ggataaacac attgcggtaa gcggttatga cagcaaccgc ctgcccg
//
LOCUS       DAZFDP010000174         8905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFDP010000174 DAZFDP010000000
VERSION     DAZFDP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(624..2084)
                     /gene="phoQ"
                     /locus_tag="ACHJ1G_004323"
     CDS             complement(624..2084)
                     /gene="phoQ"
                     /locus_tag="ACHJ1G_004323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4766070.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYIVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2084..2755)
                     /gene="phoP"
                     /locus_tag="ACHJ1G_004324"
     CDS             complement(2084..2755)
                     /gene="phoP"
                     /locus_tag="ACHJ1G_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4766071.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(2923..4293)
                     /gene="purB"
                     /locus_tag="ACHJ1G_004325"
     CDS             complement(2923..4293)
                     /gene="purB"
                     /locus_tag="ACHJ1G_004325"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4766072.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4297..4938)
                     /gene="hflD"
                     /locus_tag="ACHJ1G_004326"
     CDS             complement(4297..4938)
                     /gene="hflD"
                     /locus_tag="ACHJ1G_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4766073.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(4974..6080)
                     /gene="mnmA"
                     /locus_tag="ACHJ1G_004327"
                     /gene_synonym="trmU"
     CDS             complement(4974..6080)
                     /gene="mnmA"
                     /locus_tag="ACHJ1G_004327"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4766074.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6134..6595)
                     /gene="nudJ"
                     /locus_tag="ACHJ1G_004328"
     CDS             complement(6134..6595)
                     /gene="nudJ"
                     /locus_tag="ACHJ1G_004328"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO4766075.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(6605..7258)
                     /gene="rluE"
                     /locus_tag="ACHJ1G_004329"
     CDS             complement(6605..7258)
                     /gene="rluE"
                     /locus_tag="ACHJ1G_004329"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4766076.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7430..8680
                     /gene="icd"
                     /locus_tag="ACHJ1G_004330"
     CDS             7430..8680
                     /gene="icd"
                     /locus_tag="ACHJ1G_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4766077.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(8783..>8905)
                     /locus_tag="ACHJ1G_004331"
     CDS             complement(8783..>8905)
                     /locus_tag="ACHJ1G_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4766078.1"
                     /translation="NKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
BASE COUNT         2187 a         2243 c         2237 g         2238 t
ORIGIN      
        1 cggcggtaac tcactgagat taaagcaaag cgcaaaattg cgcggtcggg cagaatgacc
       61 accacctcaa tcgtgacgga gagtgacaac cagtgacaca ccgtgacaaa aagacggttt
      121 aaccgttcat taaaaatcga cgtgattttg tgctttccgg atgacgtggc caacgtcatt
      181 ttttagcgaa aaattctgat taaggtcact ggctgaatgg tcataatgac cacgcagacc
      241 atcgaggcaa ccatagcgat catcgttacg gttggagctt ttccgccatt ggtggaaaag
      301 gtgcacatct ggatcatcat tacggtgatc aagggtatgt aaaacccacc agcctgatta
      361 aaggttaacc ggaaaaaaac aggtggcccc gttatgggga tccccatatc gacattaacg
      421 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
      481 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
      541 tttgctgttt ttagcacttt ttgcagtgtg agttgtttaa ttaaatcaat tggttgcctt
      601 gctgtggtga taaaaggaca tatttattca tctttcggcg cagaatgctg gcgaccaaaa
      661 atcacctcca tccgcgcacc gcccagcatg ctctctccgg cgacgatttt accctcatat
      721 tgctcggtga tttcgcgggc taccgccagc cctacacctt gcccagggcg taaagtatca
      781 acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca
      841 tcctcgacca caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg
      901 aggcaatatt tacaggcatt atccagcacg ttgcccatca cctcgacaaa atcgttctgc
      961 tcaccgacaa agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga
     1021 tacactttgt tcagcgctga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg
     1081 cggctgagca atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc aatttgctgt
     1141 gaaatgcggc tgatttgctc cagcattacc ggctcagcat cactgacgct catcttttca
     1201 ctacgcagag aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc
     1261 aggtcggtga gcgtcgtacg gtatttgtcg taacgttcgc gttcactttt taacaatcgg
     1321 ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg
     1381 ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac
     1441 caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag cacatagata
     1501 aaccagctcc agactatata ggaacttttt agctccaccg gaatggtatc caccaccaca
     1561 atggttaatt ttggcatccg cgatgttgcc gggtagacgt ttactgccac cgagtgggtc
     1621 atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct
     1681 ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat
     1741 ttcagccagt caggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa
     1801 agctgcccgt tctcatcata aattagcgtc atggtggggc tttgcttgtc gatattttcg
     1861 ggtaactcga catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc
     1921 tcgccacgta acagccgaaa cgtagtttta tcgaaactga cgctataacc gatcagcgcg
     1981 accattccgt aggcaagcga aagcaccagt actaccgcag ccgttgccaa cagaaaacgt
     2041 acccgcagtg agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca
     2101 aatagccctg gccgcgaacg gtagtaatca cttcttgcgg atattgtgcc tgaatttttt
     2161 tgcgcagacg tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat
     2221 agagttggag cattaacgaa tctttgctca ccactttgcc attattgcgt atcaacgttt
     2281 ccataatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac
     2341 ggcgagagag atcaacctga aacgggggta gcgaaatgac ctgtgaagcc aggccgctat
     2401 tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca
     2461 cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctt tcacgggcgg
     2521 ttaataccag aatcggcagt gaaacatcat tgctacgcca gcggcgaatc agtgacagac
     2581 cgtcctcgtc aggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat
     2641 aataatcggc ttctttggca tcttctgcgt catcgacctg atgaccagca tcctgaatct
     2701 gaactttaag gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta
     2761 tttctccctg tcttaattat taaaatagtg taacgcgatt atgtgtttat gggggtaaac
     2821 attaaataaa ccagcgggga ggggaggtaa agtgaaaaaa gaaaaagtgg ataattttaa
     2881 taaacaggcc ggacagcatc gccatccggc actgatacga gtttatttca gctcatcaac
     2941 catggtgata gctcgaccaa tatagttagc cggcgtcatc gctttcaggc gggctttctc
     3001 ttcttctggc aacgccagac catcgataaa ctgcttcatg ccttcggcgt caacgcgctt
     3061 accgcgagtc agctctttca gcttctcgta cggtttttcg atgccatagc gacgcataac
     3121 tgtctggatt ggctcagcca gcacttccca gttgtgatcc agctcatcca gcagatggtc
     3181 acggtttact tccagtttgc tcacgccttt cagggtggat tgatacgcaa tcagcgcata
     3241 accgataccc acgccgaggt tacgcagcac ggtggagtcg gtcaggtcac gctgccagcg
     3301 ggaaaccggc agtttgcttg ccagatgctg caataccgcg ttggaaaggc ccagattccc
     3361 ttcggagttt tcgaagtcaa tcgggttaac tttatgcggc atggtggaag aaccaatctc
     3421 accagcaatg gttttctgtt tgaagtggtt aagggcgata taaccccaga cgtcacggtc
     3481 aaagtcgatc agaatggtgt tgaagcgcgc aacgcaatca aacaattcgg caatgtagtc
     3541 gtgcggttcg atctgggtgg tgtacgggtt ccactgaata cccagcgagg tgacgaactc
     3601 ttcgctgaac tgatgccagt caacttccgg gtaagcggcg atatgggcgt tatagttacc
     3661 gaccgcgccg ttgattttgc cgaggatctc cacctgatta agctggcggt actggcgctc
     3721 catacggtag gcgacgtttg ccatctcttt accgatggtt gacggcgtgg ctggctgacc
     3781 gtgggtacga gacagcagcg ggatatcgcg gtactgaacg gcgagatctt taatgccatc
     3841 aatcagttga cgccagtacg gcaggatcac ttcatcacgc gcggttttca gcattaatgc
     3901 gtgggagagg ttattgatat cttccgaagt acaggcaaag tggatgaatt cagaaaccgc
     3961 gtgcagttcc gggatctccg ccactttttc tttcaggaaa tactcaaccg ctttaacgtc
     4021 gtggttagtg gtacgctcga tggttttgat gcgtgcggca tcttcttcgc tgaaattggc
     4081 gacaattgca tcaaggtaac cgattgcgtc ggcagcaaaa gcaggaactt ccttgatcgc
     4141 tgcgtgcgcg gccagttttt gcagccaacg tacttcaact tgtacacgga atttcagcaa
     4201 accatattcg ctgaaaattc cgcgcagcgc gctgacttta tcgccgtagc gtccatcgac
     4261 aggggaaacg gcggtcagtg aggataattc catagatcac aactccgggg ttaaatgagc
     4321 aagaatttgt tttgcctgag tggtcaggcg attacgagaa aacatcagtt gcagacgtcc
     4381 gccgccgacc tggtgccaga gcacggcggc gcgaatgcct gccagcaggg ttgcgcgaac
     4441 tttcgcctgc acttgtgggc tttgcagtac agcaggggaa ccggtgacct gaatgcgcgg
     4501 gccaagcggg ctgatcacat caacatagat agcagccatc gcgctcatca gcgtttcgga
     4561 ctgtaaatcg aagtgttcga gctggcgttg caggccgttg atccggttgc ccagagtgtc
     4621 gagcgcgcct ttcgctgagg agagtttgcg ctcaagcacc atcaagctga gtgtgtagcg
     4681 ggttaattcg gcgtttaagc cctggcgact gctggcattg agcacgccga gcagggtttc
     4741 cagcccgacg cgcaggttgg cttcgctacc gccaaaaacc gccagcgtcg agctggggtt
     4801 catatcaata atactgttga gtgagacgtg tagcgcatcg gcatcacaat gcccctggtg
     4861 agcgagttgt tgcaccaggc gtgccgactg acaaataccg gccagggcga gggtgatgtc
     4921 atagtaattc tttgccacgt tcactgcttc cttgttttaa gtaaagataa taatcagacc
     4981 ggcagcggca gacgctgctc aataataccg ccaccgaggc acacttcacc gttatagaag
     5041 acggcagact ggcccggcgt cacggcggca accggttcat cgaaaatcac ttcaatgcga
     5101 tcatcgtcca gcgccttgac ggtgcaaggg atatcggtct ggcgatagcg ggtttttacc
     5161 gtgcaacgca tagtgccggt gaatggttcg cgatcgaccc agtgcaactg ctgggcaatc
     5221 aacccgacag acatcagccg cgggtgttca tggccctgag cgacaaccag aatgttgttt
     5281 tcgacgtctt tgtccaccac ataccacggt tcttcggtac cttctttggt gccaccgata
     5341 cccagacctt tacgctgacc gagagtgtga tacatcagcc cctggtgctc accaatttca
     5401 tcgccatcga cggtaatgat tttgcccggt tgcgccggga gataacggcc caggaactcg
     5461 cggaatttac gttcgccaat gaagcaaatg ccggtagagt cttttttctt cgcggtgacc
     5521 agaccaagat cttcagcaat cttacgcacc tgcggttttt ccagttcgcc gaccgggaac
     5581 aggctttgcg caatctgctc atggctgagc gtataaagga agtagctctg gtctttattg
     5641 ctgtccagac cacgcagcag gcggctcttg ccatcgacat cggcccgacg tacgtaatga
     5701 ccggtagcga tataatcggc acctaaatct tcggcggcaa attcgaggaa ggctttaaat
     5761 ttgatctctt tgttgcacag aatatccgga ttcggcgtgc gaccggcttt atattcggca
     5821 aggaacagtt cgaagacgtt gtcccagtac tcggcagcaa agttaacggt gtgcagttca
     5881 atgccgagct tgtcgcagac agcctgggca tcagccagat ccgctgccgc tgtgcaatat
     5941 tcctcaccgt cgtcttcttc ccagttcttc ataaacaggc cttcgacctg atatccctgt
     6001 tgttgcaaca gccaggcaga aacggaggaa tcgacaccgc cggacatgcc gacgattact
     6061 ttttttgcgg tttcagacat tggatcactc acgacattga acttcaaggc ggcgtattct
     6121 atcacgcagc cttttagatg acaccctttg taaaaggcca gttaaaatcg ccaatcatct
     6181 ccagcggata gcgttgcccg ctttgataac aacggatact ttccgccacc agcggcgaac
     6241 gaagatttga cgcctgtaaa atttcttcgg cgctgaccca acgacagcag tcgatatcgc
     6301 tgtcatgagg ctgggtcggg catatttgct caagctcaat ggcaaagagg aaacgcaaaa
     6361 acggcgtttt atctggcgca atccactgat gcatacgaat aaagtgttgc ggctgcgcgc
     6421 tgatgccggt ttcttcccac agttcacggg cggcggcttc cactaaggtt tcatcggctt
     6481 ccagatgccc ggcaggttgg ttccataacg ctttaccatt aatcgtctct tcaacgacta
     6541 aaaatttgcc ttctgcgtgc accacgcaag caacggtaac gtgcggttta aacattttgc
     6601 atccttaatc tgtcacttct cgccattcgc cattggcaag attatccaaa gagtaatctc
     6661 ccatcgcata gcgaatcagt cgcagcgtgg ggaaaccaac atgggcggtc atgcggcgca
     6721 cctggcgatt acgtccttca tataaggtga tcttcagcca gctggtggga atacttttgc
     6781 gttcacgaat tggtggattc cgcggccata accacgcggg ttcgtcaacc agctccgcgc
     6841 ctgcgggcag ggtagggcca tcatttaagg ttacgccatt gcgcaaggct tcaagtgcgt
     6901 cttgtgtggg aataccttcc acctgcacat aatagatttt cccggtgcgt ttgcccggct
     6961 gggttaaacg cgcctgcaac gcgccgttat tggtcagcac cagtaaccct tcgctatcgc
     7021 ggtcaagacg acctgctgca taaacgccct gaaccgggat gaattctttt aatgttttgc
     7081 gtccggcttc atcggtgaac tgcggaagaa catcgtaggg tttattgaac aggatcacac
     7141 gcgtgggctg gttttcaggt ttacgcctgg tagaacgttg cgagctgaat cgcttaactt
     7201 ggtgatttct aaaagaagtt ttttgcatgg tattttcaga gattatgaat tgccgcatta
     7261 tagcctaata acgcgcatct ttcatgacgg caaacaatag ggtagtattg acaagccaat
     7321 tacaaatcat taacaaaaaa ttgctctaaa gcatccgtat cgcaggacgc aaacgcatat
     7381 gcaacgtggt ggcagacgag caaaccagta gcgctcgaag gagaggtgaa tggaaagtaa
     7441 agtagttgtt ccggcacaag gcaagaagat caccctgcaa aacggcaaac tcaacgttcc
     7501 tgaaaatccg attatccctt acattgaagg tgatggaatc ggtgtagatg taaccccagc
     7561 catgctgaaa gtggtcgacg ctgcagtcga gaaagcctat aaaggcgagc gtaaaatctc
     7621 ctggatggaa atttacaccg gtgaaaaatc cacacaggtt tatggtcagg acgtctggct
     7681 gcctgctgaa actcttgatc tgattcgtga atatcgcgtt gccattaaag gtccgctgac
     7741 cactcctgtt ggtggcggta ttcgctctct gaacgttgcc ctgcgccagg aactggatct
     7801 ctacatctgc ctgcgtccgg tacgttacta tcaaggcact ccaagcccgg ttaaacaccc
     7861 tgaactgacc gatatggtta tcttccgtga aaactcggaa gacatttatg cgggtatcga
     7921 atggaaagca gactctgccg acgccgagaa agtgattaaa ttcctgcgtg aagagatggg
     7981 ggtgaagaaa attcgcttcc cggaacattg tggtatcggt attaagccgt gttcggaaga
     8041 aggcaccaaa cgtctggttc gtgcagcgat cgaatacgca attgctaacg atcgtgactc
     8101 tgtgactctg gtgcacaaag gcaacatcat gaagttcacc gaaggcgcgt ttaaagactg
     8161 gggctaccag ctggcgcgtg aagagtttgg cggtgaactg atcgacggcg gcccgtggct
     8221 gaaagttaaa aacccgaaca ccggcaaaga gatcgtcatt aaagacgtga ttgctgatgc
     8281 attcctgcaa cagatcctgc tgcgtccggc tgaatatgat gttatcgcct gtatgaacct
     8341 gaacggtgac tacatttctg acgctctggc agcgcaggtt ggcggtatcg gtatcgcccc
     8401 tggagcaaac atcggtgacg aatgcgccct gtttgaagcc acccacggta ctgcgccgaa
     8461 atacgccggt caggacaaag taaaccctgg ctctattatt ctctccgctg agatgatgtt
     8521 acgccatatg ggctggactg aagccgcaga cctgattgtt aaaggtatgg aaggcgcaat
     8581 caatgcgaag accgtaacct atgacttcga gcgtctgatg gaaggcgcta aactgctgaa
     8641 atgttcagaa tttggtgatg cgatcatcaa gaacatgtaa tcactacatg tgtttaatat
     8701 tgcaacgggc gtataacacg cccgttgttt tatttatgta ggtattatta atagcatatc
     8761 gagcatattt atataaaacc ctttacttga gcccatatgg gcatattttt ataatgcaac
     8821 tattatgcaa acatttattt gttattttgc tttctcctgg aggacactct tgactgcttt
     8881 tgagtagact ccataaatcc ttgtt
//
LOCUS       DAZFDP010000175         8732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFDP010000175 DAZFDP010000000
VERSION     DAZFDP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..201)
                     /locus_tag="ACHJ1G_004332"
     CDS             complement(<1..201)
                     /locus_tag="ACHJ1G_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766079.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKF"
     gene            complement(240..1469)
                     /locus_tag="ACHJ1G_004333"
     CDS             complement(240..1469)
                     /locus_tag="ACHJ1G_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4766080.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1768..2676)
                     /locus_tag="ACHJ1G_004334"
     CDS             complement(1768..2676)
                     /locus_tag="ACHJ1G_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFO4766081.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
     gene            complement(3159..4334)
                     /locus_tag="ACHJ1G_004335"
     CDS             complement(3159..4334)
                     /locus_tag="ACHJ1G_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO4766082.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(4289..4498)
                     /locus_tag="ACHJ1G_004336"
     CDS             complement(4289..4498)
                     /locus_tag="ACHJ1G_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4766083.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(4507..4653)
                     /locus_tag="ACHJ1G_004337"
     CDS             complement(4507..4653)
                     /locus_tag="ACHJ1G_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766084.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(4657..4899)
                     /locus_tag="ACHJ1G_004338"
     CDS             complement(4657..4899)
                     /locus_tag="ACHJ1G_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766085.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(4984..5148)
                     /locus_tag="ACHJ1G_004339"
     CDS             complement(4984..5148)
                     /locus_tag="ACHJ1G_004339"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4766086.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(5236..5499)
                     /locus_tag="ACHJ1G_004340"
                     /pseudo
     CDS             complement(5236..5499)
                     /locus_tag="ACHJ1G_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5722..5847)
                     /locus_tag="ACHJ1G_004341"
                     /pseudo
     CDS             complement(5722..5847)
                     /locus_tag="ACHJ1G_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(5849..6178)
                     /locus_tag="ACHJ1G_004342"
     CDS             complement(5849..6178)
                     /locus_tag="ACHJ1G_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4766087.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(6175..6414)
                     /locus_tag="ACHJ1G_004343"
     CDS             complement(6175..6414)
                     /locus_tag="ACHJ1G_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766088.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(6407..6610)
                     /locus_tag="ACHJ1G_004344"
     CDS             complement(6407..6610)
                     /locus_tag="ACHJ1G_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766089.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(6607..7485)
                     /locus_tag="ACHJ1G_004345"
     CDS             complement(6607..7485)
                     /locus_tag="ACHJ1G_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO4766090.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(7476..8012)
                     /gene="yfdR"
                     /locus_tag="ACHJ1G_004346"
     CDS             complement(7476..8012)
                     /gene="yfdR"
                     /locus_tag="ACHJ1G_004346"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4766091.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(8141..>8732)
                     /locus_tag="ACHJ1G_004347"
     CDS             complement(8141..>8732)
                     /locus_tag="ACHJ1G_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO4766092.1"
                     /translation="TRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSAL
                     LSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFED
                     NDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRI
                     IQLEAMQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         2175 a         2103 c         1916 g         2538 t
ORIGIN      
        1 gaacttgaag attagccatt caaatcccca agtaaaaaaa acaactaata tcatggctgc
       61 gaataatttc gcgatgttat ctgctgatgg attcatcaga acagattgtt tctgaaaaaa
      121 ttcacaaagt gagagtccga aaaaaataaa aaaaagtggt ctggaactaa atttttcaaa
      181 aactttgaat agggtttcca ttttctttac cttcaggatt atattagcta ttctgatact
      241 taggctacca gagcatttga tctttgagct tgaacgtaat cactccacca ttgcatcaaa
      301 ctctgtcgct ctatcagata ttctgcacga ttgtatgctg cgataatttc atcttttttc
      361 gagtgggcaa gcgctgcctc aagaacttca gctctgaatt taccagactc ctctgccgct
      421 gttcgtgcaa tagaacgcat accgtgagct acaagctcgc ctccgaaccc cattcggatg
      481 atagctgcgt tggctgtttg ttcatgcata tgattaagag gcgcttttat gctggggaaa
      541 acccattctc tatgcccact tattgatttc attaattcaa ggatgcgcaa agcttcttta
      601 ctcaaaggaa ctttgtgaag ctttttcatt ttcatgaaat cagcaggaat gttccaaatg
      661 ctgttggttg tatcaatatc agaccacctt gcgcgaacgg cttcacccgg acgaacccat
      721 gtcaacaatt gccattcaat tagcatacgt gtttccaacc ggattgacgc attcgtcaaa
      781 gattccataa accttggcaa ttcgcttggg ggaagggcag gcatattttg cttttttggt
      841 ttactgaatc tttgaccaag gttgtcagcc gggttgaact caataagttc ttcagtagct
      901 gcccaccgga agatttcatt cagacgtgaa atgatacggc gtagagtttc caatacgcct
      961 cgttgctcaa taggatcaag gtgttgtttt aagagcttag gtcggatctc attgataggg
     1021 acattaccca gaccgggaaa gacatttctc tctaagctgc gccagatgtc tgctgcatgg
     1081 tcttgtgaga tacctgatgt ctttaccttc tcatctaacc atttccgcgc tacggcttgg
     1141 agagtgtgct cagtagcact ctttaatgcc ttcgccttat cgttgttatg gatttgggga
     1201 tcaacaccat ttgccagaaa ggagagatat tcatcacgta aggctctggc tcttgcaagg
     1261 gtaaggtgag gatatgtccc aaggctcatt ttggttcttt tcttgctcac gggtactgca
     1321 tacctgaaat accaattttt cttgcctcct ttcgccaaag gagcgattcg tagaatcaga
     1381 ccatcaccgt caaacaagtt gatttcttta tcggctggct tggtgctttt gatttcagtg
     1441 tcagtgagct tcttagcgat ttttgccatt ttgggaccct cggtttttgg acccttctta
     1501 gtgggtccca ttcagggtgc cataactcgt agttctcagc aattctcact ggacgacaat
     1561 agacgtaaaa aagcccgcag agcttgtgct gtgtgggctt agtagacttc attgaacttc
     1621 aaacaactaa aaagtggagc tggcgggagt tgaacccgtg cccgagcaat gcttaaccca
     1681 ttgaataaca atgcattctc tgtttaaaaa tgcctcaagt gcattttgag tgcattttct
     1741 ggtcctctct tggtccatac aacgagatta agtggccact tgaagttccg gccctgcgtc
     1801 gctatctatt aacggctgac cttgaaccat tttttcaacc atatcgtttg aaggatatgg
     1861 aaccccgagc tcaaccgtat tagggttgga acggtgggtt tcaacaaacg atgttccgtt
     1921 ccattttttt gaaaggtaga ttattccttc aaaggctgca gggtcctgtt tctgagactc
     1981 taagatcatg tggaagccac gacgttctat cccgcgtaag aagctggaga agcactccct
     2041 cagtaattcg tcatccaaat ccttgaactt gataccctgc gcaagaaact cgaaataatt
     2101 aagaatatat aaaaggccat tgatggcatc cctgtactca tcggggacct tcatattctt
     2161 gtattcttct ttgtctgggt tgcagcgcca ctctgatagt tcttgtggta cataacgcat
     2221 gccccgatag aatttggtgc tgttccgaag ctgtttttgg tactccgggc ttgttcgggt
     2281 gttaatgatc atgttcagcg tgtgggacct gcgcgtgtta gcagcggacg ttatgcattg
     2341 tatccaccat cccattccaa cgagcattcc tgtaaccatg atcgaaagcg atgtttgata
     2401 ggccggtgtg taccttaaaa ttgcatatcc aataaggata atcacataac aaacagtgct
     2461 gaacatagga agaaattctg cagtgtctgg atagcgtttt ttgatgaagc agaagataga
     2521 agatattgct gcaccagaac cagcaatgac gatcagccaa tcaatgatgg cgatgctaaa
     2581 tggtagtttt ccagggtaga tataagtaaa aagtgctctg acaatcatca ttagagcaaa
     2641 aattgtagcg ctgacgttga ctaatgttct cgccatctta ttgattccta taacaaaaga
     2701 cctccgcatg ggaggcctta ttgtgctaat ggaggtttgt tttaaaattt taggttgtag
     2761 ttaaccttca ccgaaaccta cggtcagtgt ctttttcata gtgttcactc cagttgtggt
     2821 gcttcgccga tacctacgtt aagctcttga aaagtaaaaa aacataaaag ttttagaatt
     2881 tgtggagcac aggataacag tttgtactgg gactcatcaa gtactatcag tgtccttgca
     2941 ctgtcctagt gaaaatttat tgcaataaag taacttacgt gaagtcaaga aatatatttg
     3001 cactccattt cttgctatga tgtaagttat tgattttaat taaatttaca attacctaaa
     3061 tgaactatgg aaactatcaa taacatagat gatagctcat ttcacaaaaa gcaatggtag
     3121 actcattctt aattttcatc agccttaagc ctatgtactc agcttgacac aacatttata
     3181 gacgttttac catcgtattc agccaggtat ttaccgtaat tgcggaacag catttccggc
     3241 cccttatgcc ccatctgccc ggcaagccag aaaaggttta caccctggct aatgtgtctg
     3301 gtggcgaatg tgtggcgcgt ctggtacggg ttacggtagc gcacaccagc ttttttcagg
     3361 gtcggcaccc atgctttttt acggatagcg tcggcgttcg tccagggttc tcccgttttc
     3421 ggatcgctga atatgaactc acttttcata aaggtgtatt gcttctgcgc ctgcagggct
     3481 gccagcgcct cactgttcag ctccacctta cgggtaccgg cttttgtctt ggtgccttta
     3541 agtaccccta cgacactggc cgcctgaacg tgggctgtgt tcgcgatggt gtcgagatca
     3601 gcccagcgca gcgcgcacag ttctgagctc cgcagacctg tattgaaagc aaagcggaac
     3661 aggttttccc attccctgta cctgcagctc tggtaaatgg caagggtctc cgctggcgtg
     3721 aacgggtcaa cctcgtaatc gtcggcgctc gggctactgt cgatcacgtg gtaccggctg
     3781 gcgctgacga gggttaccgg gttaatggtc agcaggccat ccgtaacagc ttcatcaatg
     3841 gcgctgcgca gaaatgacag gttattcctg attgttttca gcttagtttt tcggctggct
     3901 atccaatttt ttagtatcgc aggcgtcaat tctgttacat gtagtttatg tagttctgat
     3961 agtgctgata gacatttttc gtaaccgcca atggttgacg gcgacagatt acggtttatg
     4021 caaattttca ggtattcatc aagataggac ttaatatttt tggttttctt tactacaccg
     4081 aataactcca gttttttgga attggggaaa tatttcgcat attcaaacgt tccgctggcg
     4141 atctgatttt gtatctcccc tagcaggcgc tcagcatatt ttataccacg tgtatttgcc
     4201 tcaagccgag ataggggctc cctgcaaaga acccctttat atgtgaaagt gataactagt
     4261 gttgaagcag ttttatgctt acgaatagtt actcctcttg gcagagataa taatccttgt
     4321 tctttcttgc ccattttgaa acctctatta agtcgaccca acgctcttta accccgtcga
     4381 cttttaatac atgtacccct tctttccata ttcctctttg tatccgtttg ttaacggcat
     4441 caaccgtttc tcccgcgttg cggcagtagg ttgatatagg tacgcaatcc agccccacag
     4501 ttcacctcac acaacattca gcccacgaca gtgacaccac acttcaaaca ttcgtttcac
     4561 aatttcacga cagtagaaac cgtcaacatc tcgcgtcagg tcatagcgat tgccgaatgt
     4621 cttgcgaacc cataactcaa aaaccgtatg catgtatcac ctccgttgca ttgcgcgcaa
     4681 ttttttcagg tgcttttcct gctcagtgtc cgccaggatt ttgcaatact gctggtggtc
     4741 aatatgttcg aacaggcagt tgaactcacc aatgcatacc cgcccggagt gtccgtccat
     4801 acgtcgaaag aacacagagc ggtccgtaca gcgagtaatc accacagggt atccggccct
     4861 gtccgtgtat atttgcccgc gttgaataag tgcgaacatg agattatccc cagcggcaaa
     4921 tcgaaaaaat aatcagcgca atcgatattg caacacctac aattgcaaat gcttcaggcc
     4981 aactcattga ctcacctcct gcggcggttc tggtagaggc atccagtggg ttacatcgca
     5041 ttcagggatg ctgatatcat caccaagcca cccttgacct tcagaccagc attgcacgta
     5101 atacccgtat tctgtgttca ctacgcacca ctgtgcgtcg ttcggcatta gctcactaca
     5161 gcttatccaa ccatccggag ttaccggaac ttgcggaatg gctgtctgct ctcgaacgtc
     5221 attaggcgct ataggttctg ctgccaactg actggcatat ttgttaatgg taacgataag
     5281 ctcttgctca gcctcatcca gataatcacc gatacctcgc ctgtcaccgt caaaatcatc
     5341 gaaatcggca cgaatcttgg caaccttctg gattgcggac aacacctcac taggaattac
     5401 cggatagttg gttgacgttt ccgcgatttc ccgaaaatta ttggttgacg aattcttatt
     5461 ttcccgaaag tttctggcct gaagcatggc ggcgcggcag gcattccagc cagtagcgta
     5521 atatttcgca tcctgccagc tcgtttgtac atttgagtac gctttttcct ctgtcatggc
     5581 ctctggtatt gatgccggtg caattcgtgg caggccgata tatagtggta catctcccgg
     5641 ctccatcgaa ttgacgggcc aaatatacgc atcactacca tggttaatga gcgcaatccc
     5701 cgaagtatcg atgatgccaa atggctccgc tgtcaatgca gtcaatgcaa tttcataagc
     5761 ccgacgctca atattgtctc gcacgtccag gctgcttatg cgctctttga tttctttaat
     5821 cagtttctta tcggtgaaag tggtcatctc actctccttt gatgcgaatg ccagcgacgc
     5881 gtagtgcgtg ttctaagtca atcaggcaaa gccagctgcc attttcttta ggtatcatga
     5941 catgtcgctt atcagcattt attgggtgcc catatcgaag gtcgtagcga gtcggtaatt
     6001 gaacttccct cgcttccagt tctgtaatgc gtttgttttt agcttctagt tcatccagca
     6061 gcaccagcac aacctgcggt gtgactttca tacgaaacgc aagcaatttt tgcggcgttg
     6121 ccacgacttt tattgcttct gccgcctcac gcagtgcctg atagtcaatc ttgctcactg
     6181 gttgcctcct ttacggatct gcgctgcgat gcgcgaaaaa aaagcctccc gcgtatggct
     6241 gttaagagct ggcgcgaacg ccgcgttaag aacggcagca tcacagccgt catcgatata
     6301 gagcgcaatt tttttctcca ggcgcgcttt ggcttcctgc aactgcatac cccggcacgc
     6361 acgcgggata taatcagcaa tttgagcgat agctttttcg ttctgtttaa acatgcttca
     6421 cctcgatagg cttgatggtg tctaacagca gtcggcggcg cgtattttct gcaaaatggc
     6481 ggcgtccagt ttctttgtgg taaaactcgt ttttgccaac gacccacatc cgctctgttt
     6541 ggtgcaattt ttttacctgc gggccatctt tggtgatcac ggtaccggta tgggttttta
     6601 cgattgtcat gccacgacct cttcgaattt caactccaat tggtcacccc agatttcaca
     6661 tgattcggaa cacgaaccag tatcaaaccg cttggccagc accatcgcct gatacaaact
     6721 tctgtagtcg ctgtcggcat acattctggc aatcccgtca agcgtcaggt gaccacggta
     6781 cataacgtct ttacctgttc tgcgatgacc atccctgacg tgtttgcctg taaccagttc
     6841 attaaaaacc cgcatcagac caggttcgtc tttacatgca agccccagct tttgcgttga
     6901 ctttttgatg caaaaaacac agttcccgag gtgctccggg atttgcaaat caaaaggttg
     6961 tttttgccac caccggataa catccgactt atcaaaatct gacagctcgg caagataccg
     7021 gacgccaggt ttcggtttca gcctacgggg ttcgtccgca cgaataccga gccatgtgat
     7081 gtaattccct cgcccaaaat ggtcatcgca atatttcgtg aaaggggtga gttttagcct
     7141 gtcagtacag aatgcgccgc cgatgtatgg cgtaccgtac tttttaacca tgtccataaa
     7201 cggtttaagc accggcattc gcgtctgaat atcctttggt tcccattccg tataaccatt
     7261 tggctgccca agctctggat ttatatcgac ctgcaacaca gttagcggta tgccccagaa
     7321 cttcaaaacc tcccgaataa agcggtatgt cagcggatgt tcgcaaccgg tatccataaa
     7381 gatgtagcag acgttattgc cagcctttct ttgttcttcc atcaggtgaa caagatatgc
     7441 agatgttctc ccgccagaaa aactaactac atgagttatg cacatttgcg taattccgat
     7501 aactcgttga agcgttccat aaacatcccg taggcatggc ccggtgccag tggaatcact
     7561 ttgaacatct ctgttgccgg gataccttcc aatacaggcc agaaagagcc atcatcaagt
     7621 ccgagatcgc ggcgttcggt tgccagcatg atgagatcgg catatttcac tggcgtgctc
     7681 ataacaggag gtaacccgta tttctcacgg attacggcgt ctattttttc ttccatccgt
     7741 ttatagtcag gaagaagtcg tttcagtggc gcggggatgt cctggcaata tgcttctgtt
     7801 gcatcatgca ttaacgcttc aaaagcaaat tcctgcggca ccagctggct gcaaagcacc
     7861 gcatgctggg cgacactgta gaagtgtgaa agatgtcctg caaagcgaca gatatttgaa
     7921 agggaaactg cgatatcgtt aatcacgatg tcgtctttat ttatcttgtc ataataaaaa
     7981 tgcttcccgg aaaaagtttt aataaatgac atttcgttct ccactttata tgcgctgcac
     8041 cgcgctgaat tctgctaaaa ggaagctctc accatccggt gattattgag ttaattacgt
     8101 ttccataaat gcccccgcag gggcatttgc agtaatgaaa tcaggcggtg aaagtaccaa
     8161 taaaggtttc tactttgctg tctttgaatt tctcaacaag cagatcacga aattcgttag
     8221 ccatttcttc ctgcatcgct tccagctgaa taatgcgcag aaccagtaca ggacgatcgc
     8281 cagtgataat gctgaggcgt aatttaaacg gacgttcttt caggccttca aacggaacgc
     8341 atttaaattc aaatgccact ggcataatgt ctttggtctt cgcttcgaca gactccatca
     8401 gggagcgttt gccgctgaag tcattgtctt caaaatcagc ggtctggttt gcttcaattg
     8461 tgattttacg gactgccgca gccgctttgg ttgcctgaat ggtgtcacca ttagcatcaa
     8521 agcccacaag gtagtcggcc cagtcttcaa tccattctgc cagtgacttc tgggagttac
     8581 gctcgccatt aacagacaac agagcagaaa acggtgctgt ctttttcagt ttgagggtgg
     8641 cggtgttatc tgcgtgacct ggttcatcaa tagtacccag gttaagcaca ctgacggctc
     8701 gcatattatc ggcatcgata aagcagcggg tg
//
LOCUS       DAZFDP010000176         8664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFDP010000176 DAZFDP010000000
VERSION     DAZFDP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(395..601)
                     /locus_tag="ACHJ1G_004348"
     CDS             complement(395..601)
                     /locus_tag="ACHJ1G_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4766093.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            914..1003
                     /gene="kdpF"
                     /locus_tag="ACHJ1G_004349"
     CDS             914..1003
                     /gene="kdpF"
                     /locus_tag="ACHJ1G_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4766094.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1003..2676
                     /gene="kdpA"
                     /locus_tag="ACHJ1G_004350"
     CDS             1003..2676
                     /gene="kdpA"
                     /locus_tag="ACHJ1G_004350"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4766095.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2699..4747
                     /gene="kdpB"
                     /locus_tag="ACHJ1G_004351"
     CDS             2699..4747
                     /gene="kdpB"
                     /locus_tag="ACHJ1G_004351"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4766096.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4756..5328
                     /gene="kdpC"
                     /locus_tag="ACHJ1G_004352"
     CDS             4756..5328
                     /gene="kdpC"
                     /locus_tag="ACHJ1G_004352"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4766097.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5321..8005
                     /gene="kdpD"
                     /locus_tag="ACHJ1G_004353"
     CDS             5321..8005
                     /gene="kdpD"
                     /locus_tag="ACHJ1G_004353"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4766098.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8002..>8664
                     /gene="kdpE"
                     /locus_tag="ACHJ1G_004354"
     CDS             8002..>8664
                     /gene="kdpE"
                     /locus_tag="ACHJ1G_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4766099.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1812 a         2252 c         2513 g         2087 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tggacaatgc tactgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt
      181 tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga gatatctgtc
      241 agataagaac aattaggaaa atatttatat atatttcatg aatatatgtt tcgattggta
      301 taacgatgtc taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc
      361 gctggcggtt ttcaatgtat tcaaaactca gcaatcaata aaaatcacca gttgcctttt
      421 cggcctgatc catccacacc ggtttatcgc tggttttcga ccagacgcga tgcaggtagc
      481 tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg
      541 caaccgcata agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca
      601 tacttttctc ccccttttgt atctacccgg tgaatggtac cggaaaaatg aatttgttta
      661 tctgatgaaa atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt
      721 gcttattgat ggtttattta cattcatcct gtaattaagt tacacaaaag ttaaattaat
      781 actaaacatt agttaaatca tggcttttgc catttttata ctttttttac accccgcccg
      841 cagatttttg cgaaatcttt gcagccagaa ttctaccctt ccggtatcac ttttaggcca
      901 ctggaggtgc actgtgagtg caggcgtgat aaccggcgta ttgctggtgt ttttattact
      961 gggttatctg gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc
     1021 ttactgatcg ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg
     1081 ctggcgcggc tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc
     1141 tttcgtttac ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt
     1201 ctcggcctga acatgctggg gctggcggtg ctgtttttta tgttgctcga tcagcactat
     1261 ctgccgctta atccacagca gttgccgggg ctgtcgtggg atctggcgct gaatgccgcc
     1321 gtcagctttg tcacgaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat
     1381 ttcagccaga tggctggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg
     1441 gtgatttttg ccctcatccg tgcgtttacc cgccagagca tgagcacact cgggaatgcc
     1501 tgggtcgatc tgctacgcat cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca
     1561 ctgtttttta ttcaacaagg tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc
     1621 gttgaaggag cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag
     1681 atgctcggta ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac
     1741 ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacggcgctg
     1801 tgctttgcct ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg
     1861 atgtcagtga tttttgtcat ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat
     1921 cctcatctgc tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt
     1981 ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg
     2041 gtgattgcga tgcatgattc gtttaccgct ctcggtggca tggtgccgat gtggctgatg
     2101 caaattggtg aagtggtgtt cggcagtgtc ggttctggtc tttacggcat gatgctgttt
     2161 gtcctgctgg cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt
     2221 aaaaaaatcg acgtacgcga gatgaaactg actgcactgg cgattctggt taccccgacg
     2281 ctggtgctga tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc
     2341 aaccctggcc cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac
     2401 aacggcagcg cctttgccgg attaagcgcc aactccccgt tctggaactg tttactggcg
     2461 ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg
     2521 ctggtgagta aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg
     2581 tttgttggcc tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc
     2641 ctggcgcttg gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat
     2701 gagtcgtaaa caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc
     2761 ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat
     2821 cggcagtctg ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa
     2881 tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt
     2941 cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa
     3001 aaaaactgcc tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt
     3061 tcctgccgat caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc
     3121 ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga
     3181 atcggcaccg gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg
     3241 tattctttct gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga
     3301 tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct
     3361 gactatcctg ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc
     3421 gttttccgcg aggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg
     3481 tctgatccca accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg
     3541 gatgctgggc gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt
     3601 tgacgttctg ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga
     3661 gtttatccct gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc
     3721 gctggctgat gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc aacgttttaa
     3781 cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag
     3841 ccggatgagc gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat
     3901 tcgccgccat gttgaggcta acggcggtca cttccctacc gatgttgatc aaaaagtcga
     3961 tcaggttgcg cgtcaggggg ccacgccgct ggtggtggtg gaaggttccc gtgtgctggg
     4021 cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg
     4081 caaaatgggc attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat
     4141 tgctgcggaa gcgggcgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc
     4201 attgattcgt cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa
     4261 cgatgctcca gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gtactcaggc
     4321 ggcgaaagaa gcgggcaata tggtcgatct cgactctaac ccgaccaagt tgatcgaggt
     4381 ggtacatatt ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc
     4441 caacgatgtg gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca
     4501 gttaaatgcg ctgaacatca tgcgccttca ttcgcccgac tccgccattc tcagtgcggt
     4561 cattttcaac gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta
     4621 taaaccgctt accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg
     4681 gctgctggtg ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct
     4741 ggtgtgaggt ttaccatgcg tggcttacgt ccggcattat cgacatttat ctttctgtta
     4801 ttgattactg gcggcgttta cccgctgctg accaccgtgc tggggcaatg gtggtttccc
     4861 tggcaggcca acggttcgtt gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc
     4921 gggcagaatt ttaccggcaa cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg
     4981 ccctataatc cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat
     5041 aaacaaatag ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagtacgaac
     5101 gttccggttg aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa
     5161 gcggcggtct ggcaaatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc
     5221 acgcaactga tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc
     5281 gtcaacattg ttgaactcaa tctggcgctg gataaacttg atgaataacg aacccttacg
     5341 tcccgacccc gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt
     5401 tttcttcggt gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg
     5461 actgcgggcg caagggctgg atattgtggt tggcgtggta gaaacccatg ggcggaaaga
     5521 taccgccgcc atgcttgaag ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg
     5581 gcggcatatc agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt
     5641 aatggacgaa ctggcgcaca gtaatgcgcc aggttctcgt catcctaaac gctggcagga
     5701 tatcgaagaa ctgctggaag ctggcattga tgttttcaca accgtcaacg ttcagcatct
     5761 ggaaagtctg aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc
     5821 cgatcctttt ttcgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct
     5881 gcgccagcgg ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga
     5941 acattttttc cgcaaaggca atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc
     6001 cgatcgcgta gatgagcaaa tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg
     6061 gcacacgcgc gacgcgatcc ttttatgcat cgggcataac accggcagtg agaaactggt
     6121 ccgcgcggcg gcgcggttgg cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga
     6181 aacccctgcc ctgcatcgct taccggaaaa aaaacgtcgg gcaattctca gcgccttacg
     6241 tctggcgcag gagctgggcg cggagacggc tacacttact gatccagcgg aagaaaaagc
     6301 agtagtgcgt tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc
     6361 gtgccgctgg tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct
     6421 cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga
     6481 tagccgctct tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc
     6541 gttatgcgcc gttatcactt taattgccat gcagtggctg atggcgtttg atgccgccaa
     6601 cctggtgatg ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc
     6661 ttcggtggtt gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg
     6721 cggcacgctc gctgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt
     6781 cgggctggtg atcgggaacc ttactgcggg tgtgcgttat caggcgcggg tagcccgtta
     6841 ccgcgagcaa cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag
     6901 tccgcaggac atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag
     6961 tcaggtgttg ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat
     7021 gacgccatgg gacgatgcca tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc
     7081 gggcaccgac acgttacccg gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa
     7141 aacctacgga ctggtggtgg tggaaccggg gaatctgcgt cagttgatga tcccggagca
     7201 gcagcgcctg ctagagacgt ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct
     7261 gaccgccagt gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgccct
     7321 gctggcggcg ctttcgcatg atttacgcac gccgcttacg gtgctgtttg gtcaggcaga
     7381 aatcttaacg ctcgatctgg caagcgaagg atcgccccac gcccgccagg ccagcgagat
     7441 tcgtcagcat gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat
     7501 tcagtccggc ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tagtcggcag
     7561 cgctctgcaa atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc
     7621 gctgacctta atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga
     7681 gaacgcggtg aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg
     7741 cgaaaatcta caactggatg tctgggataa cggccccggt cttccgccag gccaggagca
     7801 gacgatattt gataagtttg ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct
     7861 tggactggca atttgtcggg cgatagtgga tgtacacggg ggcactatta ccgcgttcaa
     7921 ccgaccggaa ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact
     7981 tgaagacttt catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt
     8041 cgtcgctttc tgcgcacggc gctggagggc gacgggatgc gcgtctatga ggccgaaacg
     8101 ctgcaacgcg gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc
     8161 ggcctgcccg atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg
     8221 ccggtgattg tgctttccgc acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc
     8281 ggagcggatg attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc
     8341 gtcgctttac gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat
     8401 gttaccgtcg atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcacg
     8461 ccgattgagt tccgcctgct ggcggtgctg ctaaataatg ccgggaaagt gctcacccag
     8521 cgccagttac tcaatcaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt
     8581 atttatatgg gacatctgcg acaaaaactg gaacaggatc ccgctcgccc ccgccatttc
     8641 attactgaaa ccggtattgg gtat
//
LOCUS       DAZFDP010000177         8627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFDP010000177 DAZFDP010000000
VERSION     DAZFDP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(259..522)
                     /gene="rpsT"
                     /locus_tag="ACHJ1G_004355"
     CDS             complement(259..522)
                     /gene="rpsT"
                     /locus_tag="ACHJ1G_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4766100.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            625..843
                     /locus_tag="ACHJ1G_004356"
     CDS             625..843
                     /locus_tag="ACHJ1G_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4766101.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            851..1792
                     /gene="ribF"
                     /locus_tag="ACHJ1G_004357"
     CDS             851..1792
                     /gene="ribF"
                     /locus_tag="ACHJ1G_004357"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4766102.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1835..4651
                     /gene="ileS"
                     /locus_tag="ACHJ1G_004358"
     CDS             1835..4651
                     /gene="ileS"
                     /locus_tag="ACHJ1G_004358"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4766103.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4651..5145
                     /gene="lspA"
                     /locus_tag="ACHJ1G_004359"
     CDS             4651..5145
                     /gene="lspA"
                     /locus_tag="ACHJ1G_004359"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4766104.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5233..5682
                     /gene="fkpB"
                     /locus_tag="ACHJ1G_004360"
     CDS             5233..5682
                     /gene="fkpB"
                     /locus_tag="ACHJ1G_004360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4766105.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5684..6634
                     /gene="ispH"
                     /locus_tag="ACHJ1G_004361"
                     /gene_synonym="lytB"
     CDS             5684..6634
                     /gene="ispH"
                     /locus_tag="ACHJ1G_004361"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4766106.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6700..7614
                     /gene="rihC"
                     /locus_tag="ACHJ1G_004362"
     CDS             6700..7614
                     /gene="rihC"
                     /locus_tag="ACHJ1G_004362"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4766107.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7781..8602
                     /gene="dapB"
                     /locus_tag="ACHJ1G_004363"
     CDS             7781..8602
                     /gene="dapB"
                     /locus_tag="ACHJ1G_004363"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4766108.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1953 a         2152 c         2465 g         2057 t
ORIGIN      
        1 atgtgtatca caatatccac attctttgta ggggagtctg gagattgagt agatattctt
       61 gttcagaatg tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg
      121 taacggcatg tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct
      181 ctcacgcatc ataaaagcag caggcataaa aaaacccgct tgcgcgggct ttttcacaaa
      241 gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt tagccttatg
      301 acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca cgatcggttg
      361 catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac
      421 tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac gagccttttc
      481 agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac tcccaaatgt
      541 gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct tttgtcaatg
      601 gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga tggcgcagga
      661 ttctaccagc ttgcagggtg tgaatacagc ttttccgcga taaaaattgc agcaggcggt
      721 cactttcttc ccgtgatttg cgccatggca atgaaaagcc acttctttct gatttcggta
      781 ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt ttcactgttt
      841 tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg
      901 gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca
      961 gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc
     1021 tcaaccactg gaactgtttg ctaccgataa agccccggca cggctgaccc ggctgcggga
     1081 aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc gtttcgacag
     1141 gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat cttctggtga agcatttgcg
     1201 cgtaaaattt cttgccgtag gtgatgattt ccgctttggc gctggtcgtg aaggcgattt
     1261 cttgttatta cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt
     1321 ttgcgaaggt ggcgtgcgta tcagcagcac cgctgtgcgt caggcgctgg cggatgacaa
     1381 tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc gtgtagtcca
     1441 cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc tacgccgtca
     1501 ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg aaaagccgtt
     1561 acccggcgtg gcaaacattg gaacacgccc aacggttgcc ggtattcgcc agcagctgga
     1621 agtgcatttg ttaggtgttg caatggacct ttacggtcgc catatacaag tagtgctgcg
     1681 taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc
     1741 gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt aagcctgtta
     1801 tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat caaccctgaa
     1861 tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac cgggaatgct
     1921 ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa aaggcaaaaa
     1981 aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata ttggtcactc
     2041 ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg gttatgactc
     2101 gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag tagagcaaga
     2161 atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt gccgcgaata
     2221 cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg tgctgggcga
     2281 ctggtcgcac ccgtacctga ccatggactt caaaactgaa gccaacatca tccgcgcgct
     2341 gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg aagccagttc actggtgcgt
     2401 tgactgccgt tctgcgctgg cggaagcgga agttgagtat tacgacaaaa cttctccgtc
     2461 catcgacgtt gcttttcagg cggtcgatca ggatgcactg aaagcaaaat ttgccgtaag
     2521 caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga ctctgcctgc
     2581 gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga tcgacggtca
     2641 ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg gcgtgaccga
     2701 ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag ttgctgcgct ttacccatcc
     2761 gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctgg atgccggtac
     2821 cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg gtcagaaata
     2881 cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg gcacttatcc
     2941 gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc tgctgcagga
     3001 aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct gctggcgtca
     3061 caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg atcagaaagg
     3121 tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggatcccgg actggggcca
     3181 ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc gtcagcgcac
     3241 ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc atccgcgtac
     3301 tctcgaacta atggaagaag tggcaaaacg cgttgaagtt gacggcatcc aggcgtggtg
     3361 ggatcttgat gcgaaagaga tcctcggcga tgaagctgat cagtacgtga aagtgccgga
     3421 cacattggat gtatggtttg actccggatc tacccactct tctgttgttg acgtgcgtcc
     3481 ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc accgtggttg
     3541 gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt atcgtcaggt
     3601 actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat ccatcggcaa
     3661 taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc gtctgtgggt
     3721 ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga aacgtgctgc
     3781 cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc tgaacggttt
     3841 tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc gctgggccgt
     3901 aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat acgatttcca
     3961 cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct tctacctcga
     4021 catcatcaaa gaccgtcagt acaccgccaa agcggacagc gtggcgcgtc gtagctgcca
     4081 gactgcgctg tatcacatcg ctgaagcgct ggtgcgctgg atggcaccaa tcctctcctt
     4141 caccgctgat gaagtgtggg gctatctgcc gggcgaacgt gaaaaatacg tctttaccgg
     4201 tgagtggtac gaaggcctgt ttggtctggc agacagtgaa gcgatgaacg atgcgttctg
     4261 ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga
     4321 taagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac cggaactggc
     4381 ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct ccggcgctac
     4441 cgttgcagac tataatgacg cacctgctga tgctcagcag agcgaagtac tcaaagggct
     4501 gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc actacaccca
     4561 ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc
     4621 cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc aacagggcta
     4681 cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa atacctgatc
     4741 ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat
     4801 gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg
     4861 ttctttgccg gtattgcgat tggtattagc gtgatcctgg cagtgatgat gtatcgctcg
     4921 aaggccacgc agaagctaaa caatatcgct tatgcgctga ttattggcgg cgcgctgggc
     4981 aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt ctacgtcggc
     5041 gactggcact tcgccacctt caaccttgcc gatactgcca tttgtgtcgg tgcggcactg
     5101 attgtgctgg aaggtttttt gccttctaaa gcgaaaaaac aataataaac cctgccggat
     5161 aaggcctaca gattgctgcg aaatcgtagg ccgcatccgg caaaaatcct caaaatataa
     5221 gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt gcacttcacg
     5281 ctgaaactcg atgatggcac caccgctgag tctacccgca acaacggtaa accggcgctg
     5341 ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgtt ggggctgaaa
     5401 gtgggcgata aaaccacctt ctcgctggag cccgatgcgg cgtttggcgt gccgtcaccg
     5461 gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc agaaattggc
     5521 gcaattatgc tttttaccgc aatggatggc agtgagatgc ctggcgtgat ccgcgaaatt
     5581 aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac cgttcatttt
     5641 gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga tcctgttggc
     5701 caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg aaaacgcgct
     5761 ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtacataacc gctatgtggt
     5821 cgatagcttg cgtgagcgtg gggctatctt tattgagcag attagcgaag taccggacgg
     5881 cgcgatcctg attttctccg cacacggtgt ttctcaggcg gtacgtaacg aagcaaaaag
     5941 tcgcgatttg acggtatttg acgcgacctg tccgctggtg accaaagtgc atatggaagt
     6001 cgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggtcacgccg ggcacccgga
     6061 agtggaaggg acaatgggcc agtacagtaa cccggaaggg ggaatgtatc tggtcgaatc
     6121 gccggacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct ttatgaccca
     6181 gaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta aacgcttccc
     6241 gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc gtcaggaagc
     6301 ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga aaaactcctc
     6361 caactccaac cgtctggcgg agctggccca gcgtatgggc aaacgcgcgt ttttgattga
     6421 cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg gcgtgactgc
     6481 gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc agcagctggg
     6541 cggtggtgaa gccattccgc tggaaggccg tgaagaaaac attgttttcg aagtgccgaa
     6601 agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa gttatgcgaa
     6661 aatgccggtc ttgttaccgg cattttttat ggagaaaaca tgcgtttacc tatcttcctc
     6721 gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt tgcacccgaa
     6781 ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa aactacccgc
     6841 aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca aggggccgct
     6901 gtgccgctgg tacgcgcacc gcgtgatgcg gcatctgtgc acggcgaatc gggaatggct
     6961 ggctacgact ttgttgagca caaccgaaag ccgctcggga taccggcgtt tctggcgatt
     7021 cgggatgccc tgatgcgtgc accagagcct gttaccctgg tggccatcgg cccgttaacc
     7081 aatattgcgc tgttactttc acaatgcccg gaatgcaagc cgtatattcg ccgtctggtg
     7141 atcatgggtg gttctgccgg acgcggcaac tgtacgccaa acgccgagtt taatattgct
     7201 gccgatccag aagctgctgc ctgtgtcttc cgcagtggta ttgaaatcgt catgtgcggt
     7261 ttggatgtca ccaatcaggc aatattaact cctgactatc tctctacact gccgcagtta
     7321 aaccgtaccg ggaaaatgct tcacgccctg tttagccact accgtagcgg cagtatgcaa
     7381 agcggcttgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc ggacctgttc
     7441 actctcaaac cctgttttgt ggcagtggaa actcagggcg aatttacctc aggcacgacg
     7501 gtggttgata tcgacggttg cctgggcaag ccagccaatg tacaggtggc attggatctg
     7561 gatgtgaaag gcttccagca gtgggtggct gaggtgctgg ctctggcgtc gtaacctgtc
     7621 acatgttatt ggcatgcagt cattcatcga ctcatgcctt tcactgatat ccctccctgt
     7681 ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg cgctggttac
     7741 tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg caaacatccg
     7801 cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg cggcgctggc
     7861 attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt tactgggcag
     7921 cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa gcagcctcga
     7981 tgcggtaaaa gatgattttg atgtgtttat cgattttacc cgtccggaag gtacgctgaa
     8041 ccatctcgct ttttgtcgcc agcatggcaa agggatggtg atcggcacta cggggtttga
     8101 cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat attgcgattg tctttgccgc
     8161 caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag ccaaagtgat
     8221 gggtgactac accgatatcg aaattattga agcacatcat agacataaag ttgatgcgcc
     8281 gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata aagatctgaa
     8341 agattgcgcg gtctacagtc gtgaaggcca caccggtgaa cgtgtgcctg gcaccattgg
     8401 ttttgccacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt ttgccgatat
     8461 tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg ctaacggcgc
     8521 ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata tgcgagatgt
     8581 gcttgatctc aatagtttgt aaccacaaaa tatttgttat ggtgcaa
//
LOCUS       DAZFDP010000178         8611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFDP010000178 DAZFDP010000000
VERSION     DAZFDP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(408..1169)
                     /gene="envY"
                     /locus_tag="ACHJ1G_004364"
     CDS             complement(408..1169)
                     /gene="envY"
                     /locus_tag="ACHJ1G_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4766109.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1352..2242)
                     /locus_tag="ACHJ1G_004365"
     CDS             complement(1352..2242)
                     /locus_tag="ACHJ1G_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4766110.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2243..5215)
                     /gene="nfrA"
                     /locus_tag="ACHJ1G_004366"
     CDS             complement(2243..5215)
                     /gene="nfrA"
                     /locus_tag="ACHJ1G_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO4766111.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5202..7439)
                     /gene="nrfB"
                     /locus_tag="ACHJ1G_004367"
     CDS             complement(5202..7439)
                     /gene="nrfB"
                     /locus_tag="ACHJ1G_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4766112.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAGLAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7708..>8611)
                     /locus_tag="ACHJ1G_004368"
     CDS             complement(7708..>8611)
                     /locus_tag="ACHJ1G_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4766113.1"
                     /translation="SCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRHSYDKSRR
                     RGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDI
                     AEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNPEHDSYAISEKSHGREEIRLH
                     IVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFA
                     TAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         2013 a         2181 c         2204 g         2213 t
ORIGIN      
        1 gaatgattat cacaatgcgc tatctgtttt tggtttaatt atctgttatt gttttatgtt
       61 gcggttttac tgtgtggttt tttatgcttt tgtggtgttt ttatctattt aagcgccatg
      121 cctttaaagg catataagcg aaaatagcat gaggtttatc ctcaattgct atgtttttta
      181 gtacaaaaaa gagggacaaa actgagacac ataaggcctc acaatggctt gcaaggcttt
      241 acatgttttg aggtagtggg acgtgtgagc gcagagatgg cgcggtaatt tgttgactta
      301 aaatgtcgtt ctaggaactt ctaagtcgtg ggccgcaggt tcgaatcctg cagggcgcgc
      361 catttcttct tcatttatgc ccgtcttatc cgtttccgct ttgcccttca ccacatcact
      421 ttttgtcgct gtttggcgag ataattcaac ggtgtcaggc cgtaaaacgc cttaaaaaca
      481 gagataaagt acgacgtgct gctatagcca cataattgcg ccacctgagt gatatttttg
      541 ttatccatca ataacatctg tacggcgtaa cgcatacgac actctgtgac aatctggcta
      601 tagctggtat tttcgttttt taatttcttt ttgagtaggc tggggcttaa acatagcgaa
      661 ctggcgacaa ttcgcagatt ccagtaatgc tgaatatcgc tttgaataat gcggcagacg
      721 ctgtcgcgga cgctgctgcg taagatatac atcagtaggg ctataaaccg cgattgctca
      781 agaaagttag acaatacggt aaaaagtaat gcgcgcgtca actccgcttc gcagggtttg
      841 ctgggttgct gcttgctgtg gttggcggct agccggaaca cttccggtgt acggctatga
      901 caagcgataa ccggggttgc cagccgcgac cagggcttta cacaggttaa gttcttattt
      961 aaaaacaaca gataatcgtt gatgatgtta cggttgatgt gggggattaa tacgtgattt
     1021 aacgttgaga gttcaatgac gttattgttg cacgcgaagg cggccaggta gtttttcgga
     1081 agggtaaacg tagcatggtt attgacgctt acctctacct ctttttcggt caggatcacc
     1141 acgcaaggtt cactgctgct caattgcatt tcgcactcct cagatatcag aaactccgct
     1201 caaaggatct atgcttcctg catgagtgat cggcccgttc gccgataacg atcttctttc
     1261 tttagcacgc tttttagcaa ttaatcttga tggaattctg acgagagcga aagaggtaag
     1321 ccaggtcgta cccgacttac ctggaggaga tttaatactc gagaatgccg tgcgcgacgg
     1381 gcaaatagcg cagagagaaa tagagagtgt ctttaccgca agaggaacgt ttcgctaaca
     1441 gcgaggcgat ctctgcatcc ggtttcggtt tcgctgtaaa ggttttgcct ttgccggcga
     1501 caaacagttc ataaacaatg ccgctggcag gggcgggact ttggcaatcg gcgctggcct
     1561 gtagataacg ttcacgctgt tcagcggtaa gtttatccac gccgctgcca tcctgtaccc
     1621 agacattaac gccggttgct ttaacttgtt ccagcagttg gcgatagcca tcgggcgaca
     1681 tgtttccggc gaaaaaacta ctgatataaa ccggttttgc tgaaacatcg ctaatcagcc
     1741 gctgcgcgtt gtttaaccat gttagcaaag gctgacgggc agcttcgctg cgccagttca
     1801 ggtcgtcaat ttccgcgctg atgtaccagc catccggcgt tacgccaggc gcggcgctcc
     1861 ataatctggc ttgctggaga tcggcagcca gcaggcgatt aagatagctt tccagcgctg
     1921 cggacgactg tttctggtgc ataaaaaatt ccggatcggc gttcagcccg acaatcagct
     1981 tcaggccagc ctgttgcgca gctgcggcac gcttaaacaa taacgcgcgc tgttctggct
     2041 gggtgaatgc atcgccgtaa cgggtccatt gcaaaaccag ggtatcgaag ccttgcaaac
     2101 gtaactgact catcagcccc tgccactggg tatcggtaac ctgactatct cggttttgtg
     2161 gttgccagat aatacctttc atcgcaaagg aaaaagggct gaccaaaagc agtgtcagca
     2221 atacgaaaat gaacttacgc atttaccagt gcactccaat ggtgagaaac gcgttgttgc
     2281 gctctccgtt acgttgatta atcgccttaa aggtatgttg atactcgacg ccgagactga
     2341 ctttgtgcgg ccaggcgtcg tagtgcgtct cgccggtcca gatattccag cggaccccga
     2401 ctccgccaag ctgcgcgccc tgagtgcctt tatcacgata gccgttgtcc tgaacgtgag
     2461 cgtaaggctc aatagtctgt ccgttagcta ccttctgatg ccagctgacg cgataatctg
     2521 ccgtccacgc ctgaatatcc tggcggatat attgcgccgc atcgaggtac aggttttggg
     2581 caaaccagcc tgaaccgttc gggtgccatt cgtcgctgta tttgccgcca ttaaagaatg
     2641 aggcgctggc gcgcagcatg gtatcggatg cgccattttg gccgttcagc ggcaactgct
     2701 gttcgacggc gaggaaaaag atctgatcgc gcagcggctt ccagcgcaga ccggtgccgg
     2761 acatcggatt tttcaccggc atcatcaccc cgttttctcc ggtatcggca aagacgcggc
     2821 tataaactga gagcaggtcg ccttccagca gcatattgcg tccgatgcgg tactcggctt
     2881 ccagttgccc gtagctacga tagcttttcc ctggcgctgc gccgccgaca ttattgttag
     2941 cggtgctcat tgccccggaa cgcaagccga tagaagaatc gaaactgaac gtccagcggc
     3001 gaccgacctc ctcatgcaaa cggcggaaat tgaagcgttg ctgattttgc tctggggtca
     3061 gtggggttat cagcgcctga ttatcaatgt catcaatcac cagccgggcg tagtgctgcg
     3121 tcgcaggcat gtcatccaga cgctggttca cgtaggccag ttgtcggatc agtgccggat
     3181 cgtccggaag ccctttatgc gcctgttcga gcatttcccg cgactgtgcg atatcaccgc
     3241 tatcccacaa ggcgtaacca agcgctgcct ggatgttgct attattcggt tccagttcca
     3301 gcgcggcgcg caaatcactc accgccgccg ggacattatg acgttggcga taaattgtcg
     3361 cccgcgcaac gtaagcgttg gcagaaggcg caatattgat tgagcgcgtg agatcgctca
     3421 gtgcgagttc cggctgacca ggaatgtaac gttgcgcatg cagccaccag tagagggcat
     3481 tgtttcccag tccacgttgt tctgcctgtt gtagccagcg atcgcgagct gcaccatttc
     3541 ctgccgcctg ggcggtattg gcagcagcaa gcagatcctc attgctcatg tcgtgaagac
     3601 tgattttctg ccaggccgcc agtgcggtgg cgtagtcctc aacctgatac gcctgatagg
     3661 ctaccgcacg atgttgccag gcgttcggtt gtcgttgttc ggcctgaagc catgcataca
     3721 aggccacacc gggtagcgtg tcccgataac actttgccag acggttccag gcggcggcat
     3781 cgtaggaagg cgacatatcg cccagcaagc gaactattgc cgggcaatta tctgcaatac
     3841 ccggcaactg gctttgccac tgacgttgcg ccgccagcgg caagggtttt gataaaatcg
     3901 ccaccttcgc tggagttgcc aggtaaggat gactttccag cagagacgcc agtcgcgcca
     3961 ttaaagtctg gctgacacgc gcatcgccct ggaaaggata gcgttgcagc aataaatcgg
     4021 cagcttcgcg tgactgctcg ttctgcatca gttgccaggt cagttgatct aggcgggtaa
     4081 gatttgccgg ttcttgctga tacagcaatc gtgccagacg cagagattca gccttgttac
     4141 gggtcgccac gctgacagca taacgctcct caagcatttc attggcgggg agggtggcga
     4201 gcagtttttg cgctgcgtcg tactgacctt cttttaacag caccggtagc gtcgcgccaa
     4261 caacatactg gcggttatcg gcaaactgca ccgtataatt cgccaatgcc tgaacggggt
     4321 tggcgctgta tttagataac agatagagcc aacttttctc ttgtgcgtcc gtggtaaaca
     4381 gcggcttatt ttcaatgaga taatgctgga ggcgtgcttt ttcgccacga taagccagcg
     4441 cggtcgcgta agtaatatat gactgaggat cggtgaagat cccctgtgat tgcagtgcca
     4501 ggatccgatc gtccagctgc ccggcaagaa gcacgtcaaa ccactgacgg cgttctgccg
     4561 cgcttaatgt gttctgctgg cgtgcttcat tgtatagcgt atctgcctgg gaccattgtt
     4621 tcaggtagat tgcccgttgc agcagatcgg ttcgcagcgt ttttccttcc ggcgatgcag
     4681 caaacgtcgc atcgttcagt tgcgctctgg cgacaggtaa ctgtgccagc cgcagggcat
     4741 tctgcccgac ttcactgcga caacgcaggg tcggcgcagc atcgcacgct ttttgctggg
     4801 caagcagttc ttcaacagtc gtaacgcttt tcacttcaac tggaatagcc gccagactgc
     4861 gctcaagtcg ggcatctcct gggtgacgtt tcagttgatc ctccagcaac agccgcgccc
     4921 ggtcatcatg accaaaatgg cgataggctt ccgcaaggta taaagtcagc ggaatattat
     4981 ccggcacctg ctggtgtata tattcaaatt cgcggatggc ggttgcttcg tcgttatttt
     5041 tctgtgcctt cagcgcctta tcgagacggg gataaataac aaaatggcga taatcgctca
     5101 gccccagctc ttctgcgctg gtgccgatat tgtctgcgag tgcgctggta ctcaataaag
     5161 acgtcagcag taaaccagac catccgatga cgcgattaag gttattctcc ttcattttcg
     5221 gactccagtt gcgcaacctg ttctgtgttt aaacctgctt tgagtaatag tgattgcatc
     5281 gaaacttgta attcgcgttg aattgtcagg acgcgatcca acgtttcctg gctgataacg
     5341 ccttcggtga ccaaaaactt gccgagcggc agagaactgc gttcatggcg caataacaac
     5401 acgttaattg ctgaacgatt aatatgaccg agcgtggtca gtatttcggc gaacaggaac
     5461 tgatgcggca catattgccg ccagatttca ccggtctgct gttccgtgag ccactgatgc
     5521 tgaaccgcat tgtacaacat tgcccgcgga tcgtgaccgc gtcggcgtgc ataccagtga
     5581 cgtaaccccg tgacaatttg tccccgcaga acaatgacgt aacgcacttt gcgtccgact
     5641 ttacgcgtca gggccgccag cgaaacaggg tcaataccat cttcactgcc gacaattaac
     5701 tcatcatttt ccagacgcag cggcagtact gcataatgca gcgctacgga ggctggcatt
     5761 tcggcaatca gcgaggacgg gatctgccag gcatcgatgg attcccacgc cacgccgttt
     5821 tgctctgcca gcgcctgtgc cagctgctcg gcgctaatca gcccctgcat cagcattgaa
     5881 ccgcccaggc gtagaccttc gacgcgatta cgcagtgctg tatcgagttg ttcttcagtg
     5941 atgacctgat tttccagcag aatttggcct aacgggcgca acgagcgggt atcgccagtc
     6001 acgctgggga agtcatgcgt tgttttatcc cacgcgacgc gacgtggatc gccgtgttga
     6061 agtacctgtt ttagcgcgcg ccagttggcc atgaagttaa tcaggttgcc ccagaaaaga
     6121 cgcaggacag atagcagccc ctgcgtcaga ccgtagtagc cagtgacgaa aatcacccgc
     6181 tgcacgatgc ggttaaacat caagccaaag tttagccaca gcagggtcat taaccatgcg
     6241 ctgccgctaa aaatagaaag gaaatgccag gcatcgggcc acaaactttc atacgccagc
     6301 aacagcaaaa gctggagcat caccagcatc gcgaggaagc tgacaaagtt actgattgcc
     6361 cctttgcggt cgcgccagag aaagtagttc agcgtcaggc tggaggtcca tttgtgggtt
     6421 ttaaagccct ggaaaacaat gccgatgatc cagcgagatt tttgtcgaac tgcagtcgaa
     6481 aaggtatcgg ggaaatattc gcgcacgcag atcatgtttg acgtccgtgc gtgctgtaaa
     6541 aatttacgct gctcgcgttc tttggcttcg tccaccaccg gaaaacggac aaaaatttcc
     6601 gtcatacctt tctctttcag gcggaaacca atgtcgtaat cttcagtcag actctgcacg
     6661 tcgaaagcaa taccgtcacc gtcagctaac agagcggtca cggcgcggcg gctgaaacag
     6721 gtgccgacgc ctgcgctggg cacttgtccg gcgagggctt cacgcaccgg aacatctttg
     6781 ccatgcagct ctgaaaactc atcaatgtaa gtcatgctgg tgaagtgcgt ccattcgcgt
     6841 tcgaacggat acaccgggat ctgaatcaga tctttacgct cgaccagata gttgaacaga
     6901 cgcaattcca tcggtgaaat cacatcttcg gcgtcatgca gaataaaacc agcaaaagcg
     6961 aaattggcgc tacgttcaaa ctgggtgatg gcgtccagca cgttgttcag acagtcggct
     7021 ttgctggtgg gaccaggacg cgcgcagact accttatgca cgttcggaaa gcgagcgcac
     7081 acttcgtcaa catcacgttg agtatcgggg tcgttggggt aggtgccaac aaagatatga
     7141 tagttttcgt agtcgagcgt ggtcgccgcc agcccggcca tattgccgat gacgcccgtt
     7201 tcattccacg ccggaaccat aatcgctaac ggtttttcat ctggtttata cagttcgcgg
     7261 taactcattc gcgggtagcg gcgataaaca ctcaacttgc gtttaatgcg gcgtacccag
     7321 tagacgacat caataaaaaa atcgtccagc ccgctgatga acatgatgac cgctaacgtt
     7381 atcgcgatta cttttaagcc gtagagccag gttgcaaaaa catcaagaag ccagtccaca
     7441 caaaaacctt acattaacgc tggttatgtt tagggtggcg tatattaagg ttttttatga
     7501 attgtgacag ctttttacca ttaataggta tgactattgc ggcacgttat ttttacactg
     7561 gttataaaag ttgccgtttg cgtagaatgt agacaaaagc atggtgtcaa ggaggggagg
     7621 ttaacgtcat agtgaaaagg agcggtggca ccgagcaggt cataatgtag tggaagtgcc
     7681 tggaggaggt gagcaggcag ggcaagatta cgaaagcccg ctccccgcaa ggactgacgc
     7741 cagatagttt ctgtccatgg ctgcttttcg catcttacgt cttaaccctg ccttgaatac
     7801 cttatcattc gttaaaatat taatagcgat gtgccgtatc cctgaaaata attctgcggc
     7861 gtttcctctt cttattttgc agtcgtcttc attcattacc acgtccagac gccagtgcag
     7921 cttattctcc acgtgccagt ggtttcggat tgctgtggcg aacttctctg cggttaaatc
     7981 agcagaactg atataatatc tgaccgtcat ttcgggctct ttcttttgtt ctgctattat
     8041 tgaccgaaag gagactgcca cgcataattt cttcagacct ttccattcaa acgtgaaatc
     8101 aataagttca tcagggacat cgcaaacaat atgaagacgg atttcttctc tgccgtgact
     8161 cttttcactg attgcgtaac tgtcatgctc tggattattt aattctttca gcggaaattt
     8221 tccctcaaag gctttattta gccgcccctg gtttcctttt accgcgaata aataatcacc
     8281 tccctgtttt tgtatcttct ctgcaatatc tttctggcaa cccatcgcat cagttgtgat
     8341 gatttttcct ttaatatcca gcatgttaag aagttctggg atagctgtaa tctcattaga
     8401 tttctcatcc gtcttgatct gtccgatgac cagactgtgc attgttgaga acgcactaat
     8461 gacatgaatc gctcccctgc ggcgactctt gtcataagag tgccggagcg tttttccatc
     8521 aattgcaatg acgtctttat catttgatga atggcagtca cgcatccagt taataaagca
     8581 ctcgtgaaat tttgcaggac tgatacagga t
//
LOCUS       DAZFDP010000179         8506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFDP010000179 DAZFDP010000000
VERSION     DAZFDP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2650
                     /gene="cas3"
                     /locus_tag="ACHJ1G_004369"
     CDS             <1..2650
                     /gene="cas3"
                     /locus_tag="ACHJ1G_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO4766114.1"
                     /translation="FDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLA
                     DYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISH
                     STLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFL
                     PEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTR
                     FFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKV
                     LDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYD
                     RLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAW
                     FADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCI
                     LEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHV
                     TKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKV
                     HRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIE
                     QSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVW
                     DDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVV
                     MPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLW
                     LATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPE
                     MANVILVNDDESCGYSATEGLIGKVG"
     gene            2748..4310
                     /gene="casA"
                     /locus_tag="ACHJ1G_004370"
                     /gene_synonym="cse1"
     CDS             2748..4310
                     /gene="casA"
                     /locus_tag="ACHJ1G_004370"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4766115.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4307..4795
                     /gene="casB"
                     /locus_tag="ACHJ1G_004371"
                     /gene_synonym="cse2"
     CDS             4307..4795
                     /gene="casB"
                     /locus_tag="ACHJ1G_004371"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4766116.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IR"
     gene            4858..5913
                     /gene="cas7e"
                     /locus_tag="ACHJ1G_004372"
     CDS             4858..5913
                     /gene="cas7e"
                     /locus_tag="ACHJ1G_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4766117.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5924..6670
                     /gene="cas5e"
                     /locus_tag="ACHJ1G_004373"
     CDS             5924..6670
                     /gene="cas5e"
                     /locus_tag="ACHJ1G_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4766118.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6652..7302
                     /gene="cas6e"
                     /locus_tag="ACHJ1G_004374"
     CDS             6652..7302
                     /gene="cas6e"
                     /locus_tag="ACHJ1G_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4766119.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7299..8219
                     /gene="cas1e"
                     /locus_tag="ACHJ1G_004375"
     CDS             7299..8219
                     /gene="cas1e"
                     /locus_tag="ACHJ1G_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4766120.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8219..>8506
                     /gene="cas2e"
                     /locus_tag="ACHJ1G_004376"
     CDS             8219..>8506
                     /gene="cas2e"
                     /locus_tag="ACHJ1G_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4766121.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDN"
BASE COUNT         2090 a         2022 c         2363 g         2031 t
ORIGIN      
        1 ttttgatttc tggggaaaaa cccgacgcgg cgagaaagac gggggcgacg actatcacct
       61 tctttgctgg cattcgctgg atgtagccgc gatgggctat ttaatggtta aaagaaattg
      121 cttcgggctg gctgattatt ttcgtcaatt agggatttct gacaaggaac aggcggctca
      181 atttttcgcc tggttgctgt gctggcacga tattggaaaa tttgcccgct cttttcaaca
      241 actttacctg ccccctgaac tcaagattca ggaaggtgcc agaaaaaatt acgaaaaaat
      301 ctctcattca acgctgggct actggttgtg gaatcattat ttaagtgaat gtcaggagtt
      361 acttccttca tcttcgctct ctccgcgcaa acttaggcga gtgatagaaa tgtggatgcc
      421 cgtgacgaca gggcatcatg gtcagccccc tgatcgtatg gatgaattgg ataattttct
      481 gcctgaagac aaagctgcag cacgagattt tctccttgaa atcaaaccac tgtttccgct
      541 catagagatc cccgcattct gggatgatga tgagggcatt gaacttataa aacacctttc
      601 ctggtatatc tctgcaaccg tcgtgctcgc agactggacg ggttcgtcaa cgcgattttt
      661 tccacgcgtc gcacatccaa tggatattaa aggttactgg cagaaaactt taattcaggc
      721 tcaaaacgcc ttaaccgtct ttcctctaaa agcaaaagtc gcgcctttca acggaattaa
      781 tacgctgttt ccttttattg agaatccgac accgttacag caaaaggtgc tggatctgga
      841 tatcagccag caaggaccgc agttatttat tctggaagat gtgactggcg caggtaaaac
      901 agaagcggcg cttatcctgg cgcacaggtt gatagctgcg gggaaagctc aggggctgtt
      961 ttttggtctg ccaacaatgg caacggccaa tgccatgtac gatcggctgg tcaaaacctg
     1021 gcttgctttc tattcgccag aatcccgccc cagcctggtg ctggcacata gtgcccgcac
     1081 attaatggac cgctttaatg aatcactctg gtctggtgat ttagtcgggt cagaagaacc
     1141 ggatgaacaa acgttcagtc agggatgtgc ggcctggttt gccgacagta acaagaaggc
     1201 gttgctggct gaaattggcg tcggcacgct ggatcaggcg atgatggcgg tgatgccgtt
     1261 caaacataat aatctgcggc ttctggggct gagtaacaaa atcctgctgg ctgatgagat
     1321 ccacgcctgt gacgcttaca tgtcgtgcat tcttgaaggg ttgatcgagc ggcaggcgcg
     1381 tggcggaaac agcgttattt tgctttctgc cacgttatct caacagcagc gcgacaaact
     1441 cgtcgctgcc tttgcgcggg gtacagaggg ccagcaagaa gctccgttcc tggaaaagga
     1501 tgattacccc tggctgacgc atgtcacgaa atccgatgtg aactcacacc gggtagcgac
     1561 gcgcaaagat gttgagcgta gcgtcagcgt gggttggctt catagtgaac aagagtgtat
     1621 tgcgcgtatc gaatcggcgg taagtcaggg gaaatgcatc gcctggatcc ggaattctgt
     1681 cgatgacgct attaaggttc atcgtcagct gcttgcccgc ggcgtcattc ctgcttccag
     1741 cctttcactt tttcatagcc gctttgcttt tagcgatcgc cagcgaattg aaacggaaac
     1801 gctggcacgc tttggtaaat attgtagttt acagagagcg tcgcaagtca ttgtttgtac
     1861 acaagtaatt gagcaaagcg tggatattga cctggacgaa atgatctccg acctggcccc
     1921 tgttgatttg ctgattcagc gagcaggacg attacagcgg catatccgcg atattaatgg
     1981 tcagttaaag cgtgacggaa aggacgagcg ttccccccct gaattgctga ttctggctcc
     2041 cgtctgggac gactctcctg gcgacgaatg gtttggcagt gccatgcgta acagtgcatt
     2101 tgtctatccc gatcatgggc gaatctggct gacgcagcgt gtgctgcgtg agcaaggtgc
     2161 tattcagatg ccacacgcag cccggcttct tattgaatcc gtctacggtg aggacgtggt
     2221 aatgccggaa ggatttgccc gcagcgagca ggagcaagtg ggcaaatatt actgcgatcg
     2281 cgcaatggct aaaaagtttg tgctgaactt caggcccggc tatgccgcca atatcaacga
     2341 ttacctcccg gaaaaactgt cgacacgtct ggctgaggag tctgtttccc tgtggctggc
     2401 tacctgtatt gacggtgtgg tgaagcctta tgccaccggt gctcacgcat gggaaatgag
     2461 cgttgtcaga gtgcgccgaa gctggtggaa aaaacatcgg gatgagtttt ctttactgga
     2521 gggagaggcg ttcaggctgt ggtgcattga acagcgacaa gatccggaga tggcaaacgt
     2581 gattttagtc aacgatgacg aaagttgcgg gtattcggct acggaaggat tgattggcaa
     2641 ggttggttga gatgtctgcg acaggactct ctgaatagtt gatacaccga aaaggatgga
     2701 aagatggaca accgacctgg gatggtcttt tttacgagag gatgcgtatg aactcgtttt
     2761 cacttctgac aaccccgtgg ttgcctgttc gttttaaaga cggaacgaca ggcaagctgg
     2821 caccagtcga tctggctgat gaaaatgttg tcgatatcgc tgcgccgcgg gcagatctcc
     2881 agggggcggc ctggcagttt ttgctggggc tactacaaac cagcttcgcg cccaaaaatc
     2941 acggtcgttg ggatgatatc tggcaagacg gactggaggc tgaaaagcta cgggaagcat
     3001 tgctgtcctt agaacacgct ttccagtttg gggcagattc cccttcattt atgcaggatt
     3061 tcgaggcgct caagggagat aaagttcagg tcgcttcgct actgcctgag attcccggcg
     3121 ctcaaacaac gaagtttaat aaagaccact ttattaagcg tggcgtgact gaacacgtat
     3181 gccctcattg ttccgcgtta gctctgttct ccctacagtt aaatgcgcca tcaggcggca
     3241 aaggctatcg caccggtttg cgcggcggtg ggccgatgac cactctgatt gaattacagg
     3301 aatatcaagg caatcaacaa acgcccttgt ggcgcaaact gtggcccaat gtgatgccgc
     3361 aggatgaagc cgacttaccg ctacccaaaa aatttgacga tctggttttc ccctggcttg
     3421 gcccgacgcg aaccagcgaa ctggccggtg cggtggtaac ccatgatcag gtcaataaac
     3481 tccaggctta ctggggaatg ccgcgccgta ttcgtattga ttttaatacc acgacagtcg
     3541 gcaactgcga tatttgcggt gaacagagcg acgcgctcct gagcctgatg acgaccaaaa
     3601 attacggtgc gaattatgcc atgtggcagc atcccttaac gccttaccgt ataccactta
     3661 aagagggtgg cgagttttac tcggtcaaac cacaaccggg cggtttaatc tggcgcgact
     3721 ggttaggcct tatcgaaacg ggtaaatcag aaaacaatac ggaacttccc gcgctggtgg
     3781 tgaaactctt taatgccagc agtctgaaac aggcaaaagt aggcctgtgg ggatttggct
     3841 atgatttcga caacatgaaa gcgcgctgtt ggtacgaaca ccatttcccg ctgctgctca
     3901 agaaaaaaga aggccagata ccgaaactgc gtctggctgc gcaaacggct tcacggattc
     3961 taagtctgtt acgaagtgca ttgaaagaag cgtggttctc cgatccaaaa ggtgcacggg
     4021 gtgatttcag ttttgtggat atcgacttct ggaacaaaac tcagcatcgc ttcctgaggt
     4081 tagtgcgcca aattgaagaa ggtcaggatg cggacgaatt actcggcaaa tggcaaaagg
     4141 aaatttggtt attcgcgcgt caggattttg acgagcgtgt attcaccaat ccatatgagc
     4201 ctgttgattt gaaacgcgtt atgaccgcgc gcaagaaata tttcacaaca tcggcggaga
     4261 agcaaagtgc taaagccgcc agggagaaaa agcaggaggc tgctgaatga gtattgtaaa
     4321 agaggagcac aaagccacgc tgcgtaaatg gcgcgaagag ttgcaggcta aacgcggcaa
     4381 tcgtgcaagt ctgcggcgta gcacaacggt taatgatgtt tgcttgtcag aagggtttcg
     4441 ttctctgctg atgcagaccc acactttgtg gaaaattgaa gcacaagagt ggcgttttac
     4501 cgcactggcg ctggtcgccg cagttagcgc caatgtaaaa gctattgatg aacggcagcc
     4561 ttttgccgcg cagctggcgg cggtaatgtc aaaggggcgt tttactcgtc tgtcggcagt
     4621 aaaaacaccg gatgaattac tgcgccagtt acgccgcgca gtcagattat taaacggttc
     4681 cgtcaatctt gactctctgg cagagggggt cttccgttgg tgccaggaga gcgacgacct
     4741 gctcaatcat caccgccgcc agcagcggcc aaccgaattt atccgtatcc gctgagcgct
     4801 ggaatattac caggctggcg acgccgataa cgaacaaaat caataaagga ctatctcatg
     4861 acaacattta ttcagcttca tttgttaacc gcttaccctg ctgccaacct taaccgtgat
     4921 gataccggtg cgccaaaaac tgtggtgctg ggtggagcaa cgcgtctgcg cgtttcctcg
     4981 caaagtctga aacgtgcgtg gcgcacttct gcactttttg aacaggcact ggcgggccat
     5041 attggtattc gcagtgggcg tattgcgcgt gaggcggcaa ctatcctgat tgagaaaggg
     5101 attgaagata aaaaagccat cgaatggtcg gcaaaaattg ctgattatct tgggaaagct
     5161 aaaaatgaca aaaaaccaaa agatccgctc actaacgccg aaactgaaca gttagtccat
     5221 atcagcccgg cagaatttga cgccgtaaaa gcgctggccc atcagcttgc agaagaaaag
     5281 cgcgcgccaa aagaggaaga tctcgcgttg ttacgcaaag accgtatggc ggtagatatt
     5341 gccatgtttg gtcgtatgct ggcgaataaa cccgagttta atgttgaagc cgcctgccag
     5401 gtagcgcacg catttggtgt cagtgaaacg attgtcgaag atgatttttt caccgccgtt
     5461 gatgatttgc gccaggcttc tgaagatgcc ggtgccgggc atcttggtga aaccggattc
     5521 ggttctgcac tgttctacac ctatatctgc attgataaag atctgctggt cgaaaacctt
     5581 ggcggtgacg aagcgttagc taatcagacc atacgtgcct ttacggaagc cgcacttaaa
     5641 gtctccccaa ccggcaaaca gaacagcttt gccagccgtg cctacgcctc ctgggcgctg
     5701 gcagaaaaag gcaccgatca accacgctct ctggcggcgg ctttctatga acccattaat
     5761 ggcacacgtc agttagatgt ggcggtgcag cgtattacaa cgcttcgcga aaacatgaat
     5821 acggtctacg aacagaagac cgaatgcgca agttttgacg tgatgaacaa acagggaagc
     5881 atgaaggacg tgctggactt tatctgcgcg taaggggcgg cttatgagcc aatatctgat
     5941 ttttcagctt catgggccga tggcgtcctg ggggggcgat gctccaggtg aagttcgtca
     6001 tacccatgaa cttccttcgc gctcagcatt gctggggcta ctggctgccg gggtagggat
     6061 tcggcgtgat gataccgaac gcttaaacgc gtttaatcgc cactattcac tggtggtttg
     6121 cgccagccgt aacccgcgct gggcacggga ttatcacacg gtccagatgc caaaagaggt
     6181 gcgtaaagca cgttatttca gccgtcgcga agagttgagc gaccctgagc ttttgagcgc
     6241 gattatctcc cggcgcgact actacaccga tgcctggtgg atggtggcgg tggcaacaac
     6301 agccgatgcg ccttacagcc tcgaacagtt gcaggacggt ttacgccatc cagtttttcc
     6361 gctttatctg gggcgaaaaa gtcatccgct ggcattacct cttgcgccgt tactgctcga
     6421 aggcaacgcg tgtgatgcct tatgtaatgc ataccaacag tatcaggatc atttccataa
     6481 actgaaagtc tcactcccga aacttcagga tgaatgctgg tgggaaggga aacacgacgg
     6541 cctggtcgcg agcaaaatat tacgccgccg ggatgttcct ttaaatcgtc agcagtggct
     6601 gtttggggaa cgcaccgtca atcaggggcc gtggctcagc aaggaggaac catgtacctc
     6661 tcaagaataa ccttacatac cggccagctt tcgcctgcgc agttgctgca tctggtggat
     6721 cgcggtgaat atgtgatgca tcagtggctg tgggatctct tccctggcgg caaagaaagg
     6781 caatttcttt atcgtcggga agaactccag ggcgcgtttc gcttttttgt actgtcgcag
     6841 gaacgcccgg cggaaagtga tacgttcacc atagaatgcc ggtcatttgc gcccgagctg
     6901 tgtaccggac aacaactttg tttcaacctg cgggcgaatc caacaatctg taaagcgggc
     6961 aagcgccacg atctgctgat ggaggcgaaa cggcaggtga gggggccggc ggaaggaagt
     7021 gatgtctggt tgcatcaaca acaggcggcg ctggactggc tggcagcgca gggggagaga
     7081 agcggtttta cactgctgga tacctcggtt gatgcctacc gacaacagca actgcggcgg
     7141 gaaaactccc ggcaactgat ccagttcagt agcgtggatt atacggggat gcttacggtc
     7201 accgatcctg ggttatttgt gcaacgcctg agccaggggt atggtaaaag ccgggcgttc
     7261 ggctgtggtc tgatgctgat caaacccgga gcagaggcgt gacgtttgta ccactgagcc
     7321 cgatcccatt aaaagatcgc acctccatga tcttcctcca gtacggtcag atcgacgtac
     7381 tggacggcgc tttcgtgctg atcgacaaaa cagggatccg aacgcatatt ccggtgggat
     7441 cggtcgcctg cattatgctc gaaccaggaa cgagagtttc ccacgcggcg gtgcatctgg
     7501 ccgccacggt gggaacactg ctggtctggg tcggtgaagc gggcgttcgc gtttactcct
     7561 ccggacaacc cggtggggcg cgggcagata aattactcta ccaggcaaag ctggctttaa
     7621 cggaagatct acggttgaag gtggtgcgca aaatgtatga attacgtttt cgtgaaccac
     7681 agccagctcg ccgttcagtg gagcagttac gtggaattga gggatctcgc gttcgccaga
     7741 cgtatgcatt actggcgaaa caatatggtg tgaaatggaa tggtcgcaaa tacgatccta
     7801 aagactggga aaaaggcgat gttgtgaatc gctgcatcag tgctgctaca tcatgtctgt
     7861 acggtatatc tgaagcggca gtattagccg cgggatatgc gcccgctatt gggtttatcc
     7921 atagtggcaa accgctttca tttgtttatg acatagccga tatcattaaa tttgattcgg
     7981 ttgtgccaaa ggcatttgaa atagcagcga ggcaacccgc agaacctgat aaagaagtca
     8041 gattagcctg tcgcgatatt ttccgtagca ctaagttaac gggcaaatta ataccgttaa
     8101 ttgaggaagt ccttgccgca ggtgaaattg aaccgccaca acctgcgccg gatatgttac
     8161 cgccagccat ccccgaacct gaaacgttgg gcgatagcgg tcatcgagga cgcggctgat
     8221 gagcatggtc gtggttgtta cagaaaatgt cccgccgcgc ttacgtggac ggctcgcaat
     8281 ctggctactg gaagtgcgtg ccggtgtgta tgttggtgat acatcaaaac gtattcggga
     8341 gatgatctgg cagcaaatta cccaactggc tggttgcgga aatgtggtga tggcctgggc
     8401 gaccaatacc gagtcgggtt ttgaatttca gacctgggga gaaaacagac gtattccggt
     8461 ggatttggat gggttacgtt tggtttcttt tcttcctgtt gataat
//
LOCUS       DAZFDP010000180         8478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFDP010000180 DAZFDP010000000
VERSION     DAZFDP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..1226
                     /gene="ompC"
                     /locus_tag="ACHJ1G_004377"
     CDS             135..1226
                     /gene="ompC"
                     /locus_tag="ACHJ1G_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4766122.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1338..2393
                     /gene="apbE"
                     /locus_tag="ACHJ1G_004378"
     CDS             1338..2393
                     /gene="apbE"
                     /locus_tag="ACHJ1G_004378"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4766123.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2467..3531
                     /gene="ada"
                     /locus_tag="ACHJ1G_004379"
     CDS             2467..3531
                     /gene="ada"
                     /locus_tag="ACHJ1G_004379"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4766124.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3531..4181
                     /gene="alkB"
                     /locus_tag="ACHJ1G_004380"
     CDS             3531..4181
                     /gene="alkB"
                     /locus_tag="ACHJ1G_004380"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4766125.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4257..5900
                     /gene="yojI"
                     /locus_tag="ACHJ1G_004381"
     CDS             4257..5900
                     /gene="yojI"
                     /locus_tag="ACHJ1G_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4766126.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6118..7764
                     /gene="mqo"
                     /locus_tag="ACHJ1G_004382"
     CDS             6118..7764
                     /gene="mqo"
                     /locus_tag="ACHJ1G_004382"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4766127.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7913..8422)
                     /gene="eco"
                     /locus_tag="ACHJ1G_004383"
     CDS             complement(7913..8422)
                     /gene="eco"
                     /locus_tag="ACHJ1G_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4766128.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2026 a         2129 c         2300 g         2023 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaaaga tgtagatggc gaccagacct
      301 acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg
      421 tggcattcgc aggtctgaaa ttccaggatg taggttcttt cgactacggt cgtaactacg
      481 gcgttgttta cgacgtaact tcctggaccg acgtactgcc agaattcggt ggcgacactt
      541 acggttctga caacttcatg cagcagcgtg gtaacggttt cgcgacctac cgtaacactg
      601 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggc aaaaacggta
      661 gcgtaagcgg cgaaggcatg actaacaacg gtcgtgaagc actgcgtcag aacggcgacg
      721 gcgttggcgg atctatcact tatgattacg aaggcttcgg tatcggtgct gcagtttcca
      781 gctccaaacg tactgatgat cagaacagcc cgctgtacat cggtaacggc gaccgtgctg
      841 aaacctacac tggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
      901 cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
      961 tcgaagctgt tgctcagtac cagttcgact ttggtctgcg tccgtccctg gcttacctgc
     1021 agtctaaagg taaaaacctg ggtcgtggct acgacgacga agatatcctg aaatatgttg
     1081 atgttggtgc gacctactac ttcaacaaaa acatgtccac ctatgttgac tacaaaatca
     1141 acctgctgga cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag
     1201 ctctgggtct ggtttaccag ttctaatctc gattgatatc gaacaaaggg cctgcgggcc
     1261 cttttttcat tgttttcagc gtacaaacac agttttttgg tgtactcttg cgaccgttcg
     1321 catgaggata atcacgtatg gaaataagct ttacccgcgt ggcactgctg gctgccgcgc
     1381 tcttctttgt tggttgcgat caaaaaccac aaaccgccaa aacccacgct actgaagtta
     1441 ccgttcttga aggcaaaact atgggtacct tctggcgtgc cagcatcccg ggcattgacg
     1501 ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc
     1561 tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt taacgattcg caaagtttat
     1621 cgccgtggcc ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg cgcattggcg
     1681 caaggaccga tggtgcgatg gatataaccg tcgggccgct ggtgaatctg tggggctttg
     1741 gcccggaaca acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca
     1801 aaaccggctt acagcacctg acggtcatta atcagtcgca tcagcaatat ctgcaaaaag
     1861 acctaccgga tttatatgtc gatctctcca ccgtcggtga aggttatgcg gcggatcacc
     1921 tggcacgctt gatggagcag gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc
     1981 tgaacagccg tggtatgaac ggtgaaggcc tgccgtggcg ggtagcgatt caaaaaccaa
     2041 ccgataaaga aaacgcggtt caggctgtgg tggatatcaa cggtcatggt attagcacct
     2101 ctggcagtta ccgtaactat tacgaactgg acggcaaacg tctttctcat gttatcgatc
     2161 cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt gacggtgatt gctccgacgg
     2221 cgctggaagc cgatgcctgg gatactggct tgatggtact cgggccggag aaagccaaag
     2281 aggttgttcg ccgggaaggg ctggcggttt atatgatcac caaagaaggc gatagcttta
     2341 aaacctggat gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa
     2401 gattgctggt ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag
     2461 ctgattatga aaaacgccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc
     2521 cgcgacccga atgccgacag cgaattcgtt ttcgccgtgc gcaccacagg catcttttgc
     2581 cgtccgtctt gccgcgccag acatgccttg cgggaaaatg tctccttcta cgcaaatgcc
     2641 tgcgaggcac tcgccgccgg atttcgcccc tgcaaacgct gccaacctga taaagccaat
     2701 gcccagcaac atcggttgga taaaatcacc cacgcgtgtc gactgctgga acaggaaacg
     2761 cctgtaacgc tggaagcctt agccgaccag gtggcgatga gtccatttca tctacatcgg
     2821 ttgtttaaag cgactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc
     2881 cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga
     2941 ttccccgaca gcagcagtta ctatcgcaaa gctgacgaaa cgctgggcat gacggctaaa
     3001 caattccgtc acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgagctg
     3061 ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg cgatattgct gggcgatgat
     3121 gacgcgacac taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc
     3181 gatctgatgt ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg
     3241 ccgctgacgt taccgctgga cattcgcggc actgcttttc agcaacaagt ctggcaggca
     3301 ctgcgcacga taccttgcgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc
     3361 aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcata
     3421 ataccctgtc atcgggtggt ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg
     3481 tcgcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc
     3541 tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggagcggta attttgcacc
     3601 gttttgcttt taacgctgcg gagcaactga tccgcgatat taatgacgtt gccagccagt
     3661 cgccgtttcg ccagatggtc acccccgggg gatataccat gtcggtggcg atgaccaact
     3721 gtgggcatct gggctggacg acccatcggc aaggttatct ttattcgccc attgatccgc
     3781 aaacaaataa accgtggccc gccatgccac aaagctttca tgatttatgt caacgtgcgg
     3841 ctacggcggc gggctatccg gatttccagc cagatgcttg tcttatcaac cgctacgctc
     3901 ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccgattg
     3961 tttctgtttc tcttggctta cccgcgattt ttcaatttgg tggcctgaaa cgtaatgatc
     4021 cgctcaaacg tttgttgttg gaacatggcg atgtggtggt atggggcggt gaatcgcggc
     4081 tgttttatca cggcattcaa ccgctgaaag cggggtttca tccactcacc ggcgactgcc
     4141 gctacaacct gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc
     4201 tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg
     4261 aacttcttgt acttgtctgg cggcagtatc gctggccatt tatcagtgtg atggcgctaa
     4321 gcctcgccag tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg
     4381 aaacggcgga taccagtctg ctggtattgc cggagtttct gggattattg ctgctgttga
     4441 tggcagtcac tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc
     4501 gactgcgtag tgaatttatc aagcggattc tggataccaa cgtcgagcgc attgaacaac
     4561 tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt
     4621 ttgtgcgtct tccggaactg gtgcagggga tcattctcac tatcggttcc gcggcgtatc
     4681 tgtggatgct gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct
     4741 ggggtggttt tgtactggtg gcgcgggtgt acaaacatat ggcgaccctg cgtgaaaccg
     4801 aagacaggct gtatacggat tttcaaactg tactcgaagg gcgcaaagag ctgactctga
     4861 accgggaacg cgccgagtat gtgtttaaca acctctacat tcctgatgcg caagagtatc
     4921 gccaccatat tatccgcgca gacaccttcc atcttagcgc cgtgaactgg tcaaacatca
     4981 tgatgttggg cgcaatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata
     5041 ccaacgttgc cgcgacctat tcgttgacgc ttttattcct gcgtacgccg ctgctttcgg
     5101 cggttggcgc attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat
     5161 tcgcgctcgc gcctttcaaa gcagagtttc cgcgcccgca ggcgtttccc aactggcaaa
     5221 cgctggagct gcgtaacgtg acgtttgctt atcaggataa cgcgttttcc gttggtccga
     5281 ttaatctcac catcaaacgt ggcgagctgc tgtttctgat tggcggcaac ggtagcggaa
     5341 aatcgacgct ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc gaaatcttgc
     5401 tggatggcaa acctgtcagc ggcgaacaac cggaagatta tcgcaaactg ttttcggcag
     5461 tgtttaccga tgtctggctg tttgatcaac tgctggggcc ggagggtaaa cccgctaacc
     5521 cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca
     5581 acgggcgtat tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc
     5641 tggcgctggc agaagaacgc gatattatcc tgctggatga atgggcggcg gatcaggatc
     5701 cacacttccg tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtcaaa
     5761 ctattttcgc catcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa
     5821 tgcgcaacgg gcaacttagc gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg
     5881 ccgttgcccg gacggcataa tattacgacg ctgacctggc ggactgctgc cgtcaggtca
     5941 atatgctaaa aatcccttca tttctaataa ccctataatt aatcaacgaa attataatgt
     6001 ttctaaaatt agaatataat ttataaacat tatttaaatg ttgttactta agtgttaccg
     6061 ttgatgccgc gcaaatctca cctgcaataa agcgactaaa agtaaggcat taacaagatg
     6121 aaaaaagtga ctgccatgct cttctcgatg gccgtggggc ttaatgccgt ttcgatggcg
     6181 gcaaaagcga aagcgtccga ggagcaggaa actgatgtac tgttgattgg cggcggcatt
     6241 atgagcgcca cgttggggac ctatttacgc gagctggagc ctgaatggtc gatgaccatg
     6301 gtggagcgcc tggagggtgt cgcgcaggag agttcgaacg gctggaataa cgccggaacc
     6361 gggcattctg cactgatgga actgaactac accccgcaaa acgccgatgg cagcatcagt
     6421 attgaaaaag cagtcgccat taacgaagca tttcagattt cccgccagtt ctgggcgcac
     6481 caggttgagc gcggcgtgct gcgtactccg cgttcattta tcaataccgt tccgcatatg
     6541 agctttgtct ggggcgagga taacgttaat ttcctgcgcg cccgttacgc cgcgttgcaa
     6601 caaagctcgc tgtttcgcgg tatgcgttac tctgaagatc acgcgcagat caaagagtgg
     6661 gcaccgttag tgatggaagg gcgcgatcca caacagaaag tagcggcaac acgcacagaa
     6721 attggcactg atgtgaacta tggcgagatc acccgccagt taattgcttc cttacagaag
     6781 aaatctaact tctcgctgca actcagcagc gaagtccgcg ccctaaagcg taatgacgat
     6841 aacacctgga ccgttaccgt tgccgatctg aaaaacggca ctgcacagaa catccgcgcc
     6901 aaatttgtct ttatcggcgc gggcggtgcg gcgctgaagc tattacagga atcggggatt
     6961 ccggaagcga aagactacgc cggtttcccg gtgggcggac agttccttgt ttcggaaaac
     7021 ccggacgtgg ttaatcacca tctggcgaag gtttacggta aagcatccgt tggcgcacca
     7081 ccgatgtcgg ttccgcatat cgatacccgc gttctggacg gtaaacgcgt agtgctgttt
     7141 gggccatttg ccaccttctc aaccaaattc ctcaaaaacg gttcattgtg ggatctgatg
     7201 agttccacca ccacctctaa cgtgatgccg atgatgcacg tcgggctgga taatttcgat
     7261 ctggtgaaat atctggtgag tcaggtgatg ttgagtgaag aggatcgttt tgaagcgttg
     7321 aaagagtact atccgcaggc aaaaaaagag gactggcgtt tgtggcaagc ggggcagcgc
     7381 gtgcagatta tcaagcgtga tgccgagaaa ggtggcgtac tgcgtctggg tactgaagtg
     7441 gtcagtgacc agcaagggac cattgccgcg ctgctggggg catcgccagg ggcgtcaacc
     7501 gccgcgccga ttatgttgga tctgctggaa aaagtatttg gcgatcgcgt ttccagcccg
     7561 caatggcagg ctacgttgaa agcgatcgtt ccgtcttatg gacgcaagct gaacggtgat
     7621 gtagcggcaa cagaacgcga gttgcagtac accagcgaag tgctggggct gaaatacgac
     7681 aagccgcagg cagcagatag tacgccaaaa ccgcagttga aaccgcaacc cgttcaaaaa
     7741 gaagtggcgg atattgcgtt gtaatgatgt gccacatccg gcatggtatg ccggatgtgg
     7801 cgtatgctga taagacgcgc cagcgtcgca tcaggcaccg gctcgggcgt tagatgtcgg
     7861 atgcggcgtg accgccttat tcggcctacg gggtgccgca cttcacagca ttttagcgaa
     7921 ctaccgcgtt gtcaattttc tcttccgcct tccagacgcg gtacttcaca tctacattgt
     7981 ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca gcatcgccca
     8041 gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc atcgtggaaa
     8101 ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc agcgttttgc
     8161 tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta
     8221 acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc acctgacgct
     8281 tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg acgctttctg
     8341 ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga atggtcttca
     8401 tttttttgct ttccttcttc aatttatagg ttttcaccga tgatgtcgct gaaggttaac
     8461 atattcatca gatttatt
//
LOCUS       DAZFDP010000181         8449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFDP010000181 DAZFDP010000000
VERSION     DAZFDP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..608)
                     /gene="yeeO"
                     /locus_tag="ACHJ1G_004384"
     CDS             complement(<1..608)
                     /gene="yeeO"
                     /locus_tag="ACHJ1G_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4766129.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNIL"
     gene            769..844
                     /locus_tag="ACHJ1G_004385"
     tRNA            769..844
                     /locus_tag="ACHJ1G_004385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:802..804,aa:Asn,seq:gtt)
     gene            complement(882..1832)
                     /gene="cbl"
                     /locus_tag="ACHJ1G_004386"
                     /pseudo
     CDS             complement(882..1832)
                     /gene="cbl"
                     /locus_tag="ACHJ1G_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1934..2851)
                     /gene="nac"
                     /locus_tag="ACHJ1G_004387"
     CDS             complement(1934..2851)
                     /gene="nac"
                     /locus_tag="ACHJ1G_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4766130.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3177..3252
                     /locus_tag="ACHJ1G_004388"
     tRNA            3177..3252
                     /locus_tag="ACHJ1G_004388"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3210..3212,aa:Asn,seq:gtt)
     gene            complement(3296..4243)
                     /gene="ldtA"
                     /locus_tag="ACHJ1G_004389"
     CDS             complement(3296..4243)
                     /gene="ldtA"
                     /locus_tag="ACHJ1G_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4766131.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(4305..5384)
                     /gene="cobT"
                     /locus_tag="ACHJ1G_004390"
     CDS             complement(4305..5384)
                     /gene="cobT"
                     /locus_tag="ACHJ1G_004390"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4766132.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5396..6139)
                     /gene="cobS"
                     /locus_tag="ACHJ1G_004391"
     CDS             complement(5396..6139)
                     /gene="cobS"
                     /locus_tag="ACHJ1G_004391"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4766133.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6136..6681)
                     /gene="cobU"
                     /locus_tag="ACHJ1G_004392"
     CDS             complement(6136..6681)
                     /gene="cobU"
                     /locus_tag="ACHJ1G_004392"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4766134.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6833..7006)
                     /locus_tag="ACHJ1G_004393"
                     /pseudo
     CDS             complement(6833..7006)
                     /locus_tag="ACHJ1G_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(6985..7173)
                     /locus_tag="ACHJ1G_004394"
                     /pseudo
     CDS             complement(6985..7173)
                     /locus_tag="ACHJ1G_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8170..8449)
                     /locus_tag="ACHJ1G_004395"
                     /pseudo
     CDS             complement(8170..8449)
                     /locus_tag="ACHJ1G_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2281 a         2251 c         1815 g         2102 t
ORIGIN      
        1 agaatattca ggctaccgtt aatcagtagc ggtattttcg tattccctgc accacgaagt
       61 gccccgctac caatcaaagt gatggcaact gctggataac tgagtaccgt cagctccaga
      121 taagtcaacg ccagtgcttt aacttctgtc gtggcatcac ccgcgacgaa atcaataatt
      181 tgttcgccaa aatgatgaat aagcgttgcc aacagtacgg caaacaacgt catgatcacc
      241 aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc gcttaccgag actaaatgcc
      301 acaacgacag tagtaccaag atcgatagca gcaaaaaaag ccataatgac catattgaag
      361 ctgtccgcca atcccacgcc ggccatcgca tcttttccca gccagctgac cagaaaagtg
      421 ctcaggacac ccatcaacag gacacaggca ttctccatga agataggaac agcaagcggg
      481 gttatctcgc gccagaacaa cactttgtag ctcttgcgtt tagcgtgcca gcgagtgccg
      541 tgaacaacct ggcgtaaagc agaggagata ttcaaagccg accttaattg cagaaagtga
      601 aaccacattt caaataatga gggagaatca gcaaagctgc aaagattttc gccaacaaat
      661 tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac agttgggaaa tttaaaaatc
      721 gggtttgaca aaagattttt cgccgttaag atgtgcctca acagcgattc ctctgtagtt
      781 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
      841 gccaaatttc tgaaaagccc gcttttatag cgggcttttt gctatatctg ataatcaatt
      901 tcctcttcac tgttctccat cacctggcgc ttgatatcct caactgacag tcctgcatta
      961 caaagttcca gaaagcgcca gacatagtta cgttgaagtt gccctcgctt cagtcccaac
     1021 catacagtat tggcatcgaa aagatgccgc gtatccagac ggattaaatt ctcttcctct
     1081 tgttcgccgc tggattgttc ggcaactaat ccaatcccca gcccaagagc aacataggtt
     1141 ttaatgacat cagaatcctg cgcacttaat acaatatctg ccagcaaacc tttgcgggca
     1201 aatgcgtcat caatacgtga gcaccccgta atcccctatc ggtaagtgat taacggccac
     1261 ttcgctattg attccagcgt caacggtgta atttgcgtca agggatgatc gagtggaaca
     1321 agcaaactat ggtgccaacg aaaccacggg aaggcgacga gctgcgggtc attactcaaa
     1381 cgctcgctgg cgataccaat atcagcttcg ccattttgca acaacgtcgc aatttcctgt
     1441 ggcgtaccct ggattagctc cagccgaacc tccgggaaaa gttcgcgaaa agctttaatg
     1501 acctcaggca agctataacg tgcctgagta tgcgtcgttg caatagtgag aacgccagac
     1561 gtatcgttgg taaacaggtc tgcaagccga cgaacattac tggcttcatt cagaatacgt
     1621 tctgcaatga ccagtaatgc tttgcccggt tcagtcatac ccagcagtcg cttacctcgt
     1681 cgaacaaata tttcgatgcc aagttcatcc cccagttccc gaatatgacg gctgacgccg
     1741 gactgtgagg taaaaagcat attcgcaacc tctgtcaggt tgtaatcctg acgtgcagcc
     1801 tcgcggatta tctttagttg ttggaaattc acggtaaact ccgggcagct cagatttccc
     1861 gttattgtta aagtctaatg cccggcataa caaataataa aaacccgcat cttattccat
     1921 cccgatataa cgcttagctc accaattgcc actgcctttt ttccatcact ggagaactaa
     1981 tcactgacat taacaactct ttcactgcct gtgcctgtgg cgataagttc gctctggcgg
     2041 gtaaatttaa tgacaaagag agactcatgg aaggcgtggt aatgcgtgac atccacccat
     2101 ttactgcgcc acataacgaa cgcgcggccg attcgggtaa tactgcaacg cccatgccgc
     2161 tggcaatcgc tgcggtaagc gtggcaatag actcaatttc accaatgact tttgccgtga
     2221 gccgccgtag ggaaaaagcc tcatcaacac gaagtctaac agcactgtaa tcactgggga
     2281 gaaagaggtt catttgcgca atagcattaa catcaacgct ttgccccggg caatcttgag
     2341 ttcctaccag gaaaagatct tctttcagca aagcctgact ggatacccca gccacagggg
     2401 aatgctcata aatcaccgcc atatcgagtt ggtgatttat cagtttttcg ttaagcactg
     2461 caccgctatt ttcatgaaga tagataacga tctccgggaa ttcagcgcga accgcttgta
     2521 ataagggcat ggtgatagat gacgcagcgg ttcctggcgc aaagccaatc gagacttgcc
     2581 ccgataatgc ctgaccaacg ttatgcaccg ccagttgggc ctgctcacac tgacgtaaaa
     2641 tggcccgcgc atgggtatag agaatttttc cggcgtctgt tggcgtaacg ccccgttttg
     2701 tgcgaatcaa aagttgttga tttaactcac cttccagtgt ggcaacctgc tggctgagcg
     2761 ctggttgtgc gatatgcaat acttcagcag cctgagtcag gctaccaata tctacaattt
     2821 ttacgaagta tttcaggcgt ctgaagttca tgttgcctcc ggtttttgag aatcggccca
     2881 agtgccgcca ttacttacaa ccagactgca agatgcttgc cagttttatt ttggcgttga
     2941 tgtacaagct aaccaactgt caaataagag attatgatag attcgtcatt ggctcttttc
     3001 accagcagtc gcgttcccct gccctataaa aggagggtat gcaccacgat ggttcattac
     3061 caataagatt gaaatctcat cactttgttg aaattggcag caaacaaaca aaaaaatgca
     3121 tttcaccctt tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct
     3181 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     3241 tcagaggagc caaattcaaa aaagcctgct ttctagcagg atttttgctt tctaattacc
     3301 aacgctcata aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgac
     3361 gttgcaacgc agcatttgcg cgattcacat caacttcttg cccgttgata aacgcccgca
     3421 aagatggggt taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgcg
     3481 acagcggctc atgaacttcc agccagttcg agccatctgg ttcagtggtg tattttactg
     3541 gctggtcgat aatttgcaca cgcgttccaa caggaacatt atcaaacaga tatttgatat
     3601 cgtcattgcg cagacgaata cagccctgac ttacccgaag tccaatacca aaattggcat
     3661 tggtaccgtg gatggcatac aacctgccaa tataaatcgc gtacagcccc atgggattat
     3721 cgggccccgc aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccgag
     3781 tgttcggcgt tggcgtccag gttggagctt cttgtttacg ttcaacggta gtcacccagt
     3841 tacgcggggt ttctcgccca gcctggccga taccaatagg aaagacttcc acagtattac
     3901 tgtctggcgg gtagtaataa agacgcatct cagcgacgtt aaccacaatc cctttacgaa
     3961 cagtggcggg caaaatcagt tgctgcggaa tggtgagttg cgagccagac ttcggcaaga
     4021 aaacatcagc gcccggattc gcttccagca tgttacttaa cccttgcccg tattgtgcgg
     4081 caaaagtctc cagcggctgg gtattgtgat caggaacagt tacagtaagc gactgcccca
     4141 ccaaacggct accctctgga ggtaatggat aagttaccgc caggctagta tggctggcaa
     4201 aaagcagagc aaatgagcaa agaatattta cacgacgcat catgtccctt tcctatgtcg
     4261 cgaaagctat ccgttaagta tagcttttat cagacttttc gtttttaact gttcaaatca
     4321 gaagtcgtat tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttatat
     4381 atcgcacagg cagcttcgat gatgggcatc gccagagctg cgccactccc ctcacctaaa
     4441 cgcatctcca tattgagata aggctccagc cccaaatgcg acagcgctat acgcgcccct
     4501 ttttctgccg acaggtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag
     4561 gctgcgagcg cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa
     4621 gcagcgccta acatcacgcc agccattccg accaaatcaa atccccccac ttttgccaga
     4681 acatcaacgc catcctgcgg atttggttga ttcaacgtaa tcgcccggcg cacaacatca
     4741 attttattag ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga
     4801 tcccggccag tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt
     4861 tcacctacac caaacagcgt gacaccgttt tttgccagct cccgcgtata acatatgacg
     4921 tccaaaagca acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg
     4981 ctacctcgtg cgacacgcat gttgataagc ccggggatag gctcagcact atcaatacca
     5041 acatcaacta cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt
     5101 ccgcgggtca tattttcagc ctgaatggct gtcacttctt ttggggaaat agcgacccct
     5161 tcctcccaga cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt
     5221 atgccattca accccggcat tcccgccagt tgtatggcaa gcgcctccag ccttcccagg
     5281 ctaccaacag gtttgagtaa cccgtcaata tgccgttgtg cacgcgacat agcggcagga
     5341 tcgatagcag ggatcgtatt cagtaaatcg gcaagtgttt gcatgtcacg tctctttata
     5401 gcagtgccag caggaagacc agttcaccaa gctctatagc agctcccagc gtatcgccgg
     5461 tttgcccgcc cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca
     5521 ccaccatagc agccacacca tgcatacccg gcaataatac ggcagcaaaa atagcggcta
     5581 agccgagtgt gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc
     5641 cttcttcccg ggcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg
     5701 cacatgctgc cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaata
     5761 ccagaatctt tgccagtaca acaaaaatca atgccagacc gccatgggtg cctaaacggc
     5821 tatcacgcat gatttccaac atgcgatcgc ggctacgtgc agaaaatacg ccatcacagg
     5881 tatcggcgag gccgtctagg tggaatcccc cggtcatcag cgccagcaca agtacgctaa
     5941 acagtgccgc cagtggtacg ccacaccatg cctgcagcac catgaagacc agcccgctaa
     6001 tcgcgccaag caataatcca attaaaggaa aagtaataat accgcgagaa taatgctcga
     6061 aatccagtcc ctgggacctg cgacgcggta caggcagacg cgtaataaat gagagcatcg
     6121 cccaaaataa tttactcatt tgatttttac tccaataccc gaaaccacca gccatacttc
     6181 atttgccgca gcggccaact gctgatttac ccgcccggca atatcacgaa aatgtcgtgc
     6241 cagacgactc tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc
     6301 ggggcaacgt tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat
     6361 cgcctgataa tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt
     6421 caccattgtg gtaacgcatt caagcaacac aacctcattc gggttaatgt ctgcatgaat
     6481 taattcatca agatgttgcc agcgctccac tgtgcgccag tgctccgggc ggccttgccg
     6541 atgatgttct atccgtgcag ccatctcatc atcaaggatt tgcgaggtag cgatatacag
     6601 aacctgtgaa gagtccccaa taagcgcctc tgcgtggcga ctcttcccgc tccgtgcgcc
     6661 ccccgtcacc aaaatcatca taccgactcc tgattcaaat gtatgatggt attcattgaa
     6721 cagaacaatg gatcccccac cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc
     6781 gtaatataat taaaaatcca gcattctcaa tccattacga aataagatgg cattacggaa
     6841 tagccagact ttctcgtgtt aacgtccgac aaaatgcgtc attgacagcg gtttctgtgg
     6901 attgacacat ttcattcaga taatgaatta atgctactgc cggaacaacc agcaaacggg
     6961 cattgtgttc tgaaatccag gctattgatt caacgtcagc gacagatctg cgctgtaaaa
     7021 gaacttgtaa aacaacgtaa tagaattatc cggaatggtg gcgactatgc actagaacac
     7081 cacaatttcg ctctctcggt aaggcactgt cacagcattc aacagaatgt gacttgccag
     7141 atttattagc gccaccagat gtttaaccgg gtaaccacga cccagtcggg gatatattcc
     7201 atcttctcgc tgacatcatc aagaataaaa aggtttatca cactcagagc aacgttcttc
     7261 agcaggcggg ataattcttc aacatttaga agatgtgtta ttaaaggcat acaaactgga
     7321 cgtattgtat tttcttttgg tggttgacct aaggtagcag tttatcctga tgcgctgaga
     7381 tttctgtaat atctacgtca acattctcct cgaatagaaa atgcagcccc ggcaagtgat
     7441 tcacattttt taacaaaacg ttgttagcgt gccattttca gaagctctcc aagaaagcga
     7501 attcttttct caaattctgc attctcagac acagctttct actccatggt aatggcccaa
     7561 atacgaagtt gctcaggatc attagtggtg ctgagaagtt aaataaccat gcggagtacc
     7621 actcgtcatc acgtatttat atatcatgaa gctattgttg tgttttgtac atcagtagat
     7681 atattctgat atactccttt tgctagacat aacctttcac ctgcttgcaa agcttctgtg
     7741 ttctgacatt gccaaattgt tgcaattctg tatccagcct tctttcagtc atagcttcgg
     7801 gccgcgataa gactcactga tctgaccctg attcctcttg cagactttat agaccaatta
     7861 aaatgcagtt tctgcaggtc aacgtctgac catcattgtc atcactctgg ccattagagt
     7921 aaccttctgc attcatcctt ttgtaaaaag tttatgtcag tatcagcaat taaccggacc
     7981 tgatactgat atgagtctta ccgcatatac ggtcaatttc agcaattaat tacattatcc
     8041 acgccaaagt gtttgtcatc gcaatgatgg taccttcttt cagacaccat tttttcaact
     8101 ccgttttcca cggaccgcac tcttatgtca agagtgcggt gatcctaccc acgtaatatg
     8161 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
     8221 actgtgccgc cgccaccgat tgtaatcaca ttcgatataa ttaaacaccg ttgcccgcat
     8281 tatttcccgg ctgataaagt gttctccatg gatacattcc actttcagcg aatgaaagaa
     8341 gctttccacg cagattatgc cgcttcagtt gcgcctgata atctgctgaa cagtactggc
     8401 ctccacggtc cgtgtgaacg ataacgttcc ggggcctctt acgccgcca
//
LOCUS       DAZFDP010000182         8396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFDP010000182 DAZFDP010000000
VERSION     DAZFDP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(182..1510)
                     /gene="dsdA"
                     /locus_tag="ACHJ1G_004396"
     CDS             complement(182..1510)
                     /gene="dsdA"
                     /locus_tag="ACHJ1G_004396"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4766135.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(1528..1746)
                     /locus_tag="ACHJ1G_004397"
                     /pseudo
     CDS             complement(1528..1746)
                     /locus_tag="ACHJ1G_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            1989..2984
                     /locus_tag="ACHJ1G_004398"
     CDS             1989..2984
                     /locus_tag="ACHJ1G_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4766136.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            complement(2992..4425)
                     /gene="cscA"
                     /locus_tag="ACHJ1G_004399"
     CDS             complement(2992..4425)
                     /gene="cscA"
                     /locus_tag="ACHJ1G_004399"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO4766137.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            4641..5555
                     /locus_tag="ACHJ1G_004400"
     CDS             4641..5555
                     /locus_tag="ACHJ1G_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO4766138.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5627..6874
                     /locus_tag="ACHJ1G_004401"
     CDS             5627..6874
                     /locus_tag="ACHJ1G_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO4766139.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(7403..7603)
                     /gene="torI"
                     /locus_tag="ACHJ1G_004402"
     CDS             complement(7403..7603)
                     /gene="torI"
                     /locus_tag="ACHJ1G_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO4766140.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(7659..7826)
                     /locus_tag="ACHJ1G_004403"
     CDS             complement(7659..7826)
                     /locus_tag="ACHJ1G_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766141.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(7862..8161)
                     /locus_tag="ACHJ1G_004404"
     CDS             complement(7862..8161)
                     /locus_tag="ACHJ1G_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766142.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(8319..>8396)
                     /locus_tag="ACHJ1G_004405"
     CDS             complement(8319..>8396)
                     /locus_tag="ACHJ1G_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 domain-containing protein"
                     /protein_id="HFO4766143.1"
                     /translation="QPGYVIVVTTQSGMTIQIHKPKEEK"
BASE COUNT         2018 a         1955 c         2063 g         2360 t
ORIGIN      
        1 ctattgcttt tatcttactt acggttttgg tttggtttgc gaaaccgccg tttacagcga
       61 aaggcgttgg gtgatgataa aaggaggggg ttatagcggg taagagcggg gatcttaagt
      121 ctgcgccatc ggcagcatcg ctcacccagg gaaaggattg cgatgctgcg ttgaaacgtt
      181 attaacggcc ttttgccaga tattgattca tctcttcttc cggcaccatt ccacctcccg
      241 tcgcccacac cagatgagtg gcattacgca attgttctgc gctgaaaccg tgcatctgtt
      301 ggtaacttac tgatgcacac acgcgctgag gtccggccat acccgccagt gctgaaggtt
      361 caagacgaat accttcttcc tgcgccagcc agccaagcat gtcatacatg gtttgatcgc
      421 taagggtata gaagccatcc agcagacgct ccattgcccg cccgacaaag cctgatgcgc
      481 gaccaactgc aaggccatcc gctgcggtaa ggttgtcgat accaatatcc tgaacagaaa
      541 tctgatcgtg taatcctgta tggacgccta acaacataca aggggagtgc gttggttcgg
      601 caaaaaagca gtgaacatga tcgccaaacg ccagtttaag tccgaatgcg acgccaccag
      661 gaccaccgcc aacaccacac ggcagataga caaacagagg gttatcagca tcgacgatac
      721 gaccttgctg ggcaaattgc gctttaagac gctggccagc gacggaatac ccaaggaaca
      781 acgtgcggga attttcgtca tcaataaaga aacagttcgg gtcagactgc gctgctttac
      841 gtccttcctc gacggcaaca ccataatctt gctcatattc cacgaccgta acgccatggc
      901 tgcgcagttt cgcttttttc catgcccggg catcagcaga catatgaact gtcatcttaa
      961 agccaatgcg ggcgctcata atgccgattg ataaccccag atttccggtt gagcccacag
     1021 caatgctgta ttggctaaag aactgtttaa actccggaga aagcagtttg ctgtagtcat
     1081 catcaagcgt cagcaacccc gcttccagag ccagtttttc tgcgtgtgcc aggacttcat
     1141 aaatcccgcc gcgtgctttt atggagccgg aaatgggcaa atggctatct tttttcagta
     1201 acagttgccc gctgatcggt tgctgatatt ctttttccag ccgtttttgc atagctggaa
     1261 tggcaaccag ttctgattca ataatccccc cagtggcagc agtttcagga aatgcttttg
     1321 ccagataggg tgcaaaacgg gataagcgcg catgggcgtc ctgaacatcc tgttcggtca
     1381 ggccaacata aggtaaacct tcagccaatg aggtcgtgcc aggattaaac caggtggttt
     1441 ctttaagagc aaccagatcc tttaccaacg gatactgggc gatgagcgag ttcattttag
     1501 cgttttccat agtacgtctc tttgcgctta gatgataaat gacagcagga atgtgcccgc
     1561 cagagcgacg actgaagcga taaatgtcgc tgtcgtatag tatttaaatg tttcattgag
     1621 cgtagcgccg caatattgct tcactagcca gaaaagcgag tccgtaacga tcgtgcagcc
     1681 aattgcacct gaaccgatag caatcgcaat aatttccggg ctgatgtcgg gatacagcgg
     1741 cagcacaatg atccatattt atattttctt ctccaggttg gcgatgccag ttggcatgtt
     1801 ttacttactt gcctatatgg ttactcgttg gcgagaacag aggcggaatt gagtacaatt
     1861 atccggcaat tattgttatt ttttattgta ttgccgtgtc gggtgatagg gcgttgagaa
     1921 tatcgttgga tctcgccagt gacgtctgtt tctgctacag tgcccgtttt acggcaaacg
     1981 gcttgggtat ggcttcatta aaggatgtcg cacgcctggc gggagtgtcg atgatgacag
     2041 tctcccgggt gatgcataat gcagaatctg tgcgtcctgc aacgcgtgac cgcgtattgc
     2101 aggcaatcca gaccctgaat tatgttcctg atctttccgc ccgtaagatg cgcgctcaag
     2161 gacgtaagcc gtcgactctc gccgtgctgg cgcaggacac ggctaccact cctttctctg
     2221 ttgatattct gcttgccatt gagcaaaccg ccagcgagtt cggctggaat agttttttaa
     2281 tcaatatttt ttctgaagat gacgctgccc gcgcggcacg tcagctgctt gcccaccgtc
     2341 cggatggcat tatctatact acaatggggc tgcgacatat cacgctgcct gagtctctgt
     2401 atggtgaaaa tattgtattg gcgaactgtg tggcggatga cccagcgtta cccagttata
     2461 tccctgatga ttacactgca caatatgaat caacacagca tttgctcgcg gcgggctatc
     2521 gtcaaccgtt atgcttctgg ctaccggaaa gtgcgttggc aacagggtat cgtcggcagg
     2581 gatttgagca ggcctggcgt gatgctggac gagatctggc tgaggtgaaa caatttcaca
     2641 tggcaacagg tgatgatcac tacaccgatc tcgcaagttt actcaatgac cacttcaaat
     2701 cgggcaaacc agattttgat gttctgatat gtggtaacga tcgcgcagcc tttgtggctt
     2761 atcaggttct tctggcgaag ggggtacgaa tcccgcagga tgtcgccgta atgggctttg
     2821 ataatctggt tggcgtcggg catctgtttt taccgccgct gaccacaatt cagcttccac
     2881 atgacattat cgggcgggaa gctgcattgc atattattga aggtcgtgaa gggggaagag
     2941 tgacgcggat cccttgcccg ctgttgatcc gttgttccac ctgatattat gttaacccag
     3001 tagccagagt gctccatgtt gcagcacagc cactccgtgg gaggcataaa gcgacagttc
     3061 ccgttcttct ggctgcggat agattcgact actcatcacc gcttccccgt cgttaataaa
     3121 tacttccacg gatgatgtat cgataaatat ccttagggcg agcgtgtcac gctgcgggag
     3181 gggaatacta cggtagccgt ctaaattctc gtgtgggtaa taccgccaca aaacaagtcg
     3241 ctcagattgg ttatcaatat acagccgcat tccagtgccg agctgtaatc cgtaatgttc
     3301 ggcatcactg ttcttcagcg cccactgcaa ctgaatctca actgcttgcg cgttttcctg
     3361 caaaacatat ttattgctga ttgtgcgggg agagacagat tgatgctgct ggcgtaacga
     3421 ctcagcttcg tgtaccgggc gttgtagaag tttgccattg ctctctgata gctcgcgcgc
     3481 cagcgtcatg cagcctgccc atccttcacg ttttgagggc attggcgatt cccacatatc
     3541 catccagccg ataacaatac gccgaccatc cttcgctaaa aagctttgtg gtgcataaaa
     3601 gtcatgcccg ttatcaagtt cagtaaaatg cccggattgt gcaaaaagtc gtcctggcga
     3661 ccacattccg ggtattacgc cactttgaaa gcgatttcgg taactgtatc cctcggcatt
     3721 cattccctgc ggggaaaaca tcagataatg ctgatcgcca aggctgaaaa agtccggaca
     3781 ttcccacata tagctttcac ccgcatcagc gtgggccagt acgcgatcga aggtccattc
     3841 acgcaacgaa ctgccgcgat aaagcaggat ctgccccgtg ttgcctggat ctttcgcccc
     3901 gactaccatc caccatgtgt cggcttcacg ccacacttta ggatcgcgga agtgcatgat
     3961 tccttctggt ggagtgagga tcacaccctg tttctcgaaa tgaataccat cccgactggt
     4021 agccagacat tgtacttcgc gaattgcatc gtcattacct gcaccatcga gccagacgtg
     4081 tccggtgtag ataagtgaga ggacaccatt gtcatcgaca gcactacctg aaaaacaccc
     4141 gtctttgtca ttatcgtctc ctggcgctag cgcaataggc tcatgctgcc agtggatcat
     4201 atcgtcgctg gtggcatgtc cccagtgcat tggtccccag tgttcgctca tcgggtgatg
     4261 ttgataaaac gcgtgataac gatcgttaaa ccagatcagg ccgtttggat cgttcatcca
     4321 tccggcaggt ggcgcgaggt gaaaatgggg atagaaagtg ttaccccggt gctcatgaag
     4381 ttttgctagt gcgttttgcg ccgcatgcaa tcgagattgc gtcattttaa tcatcctggt
     4441 taagcaaatt tggtgaattg ttaacgttaa cttttataaa aatatagtcc cttactttca
     4501 taaatgcgat gaatatcaca aatgttaacg ttaactatga cgttttgtga tcgaatatgc
     4561 atgttttagt aaatccatga cgattttgcg agaaagaggt ttatcactat gcgtaactca
     4621 gatgaattta agggaaaaaa atgtcagcca aagtatgggt tttaggggat gcggtcgtag
     4681 atctcttgcc agaatcagac gggcgcctac tgccttgtcc tggcggcgcg ccagctaacg
     4741 ttgcggtggg aatcgccaga ttaggcggaa taagtggatt tataggtcgg gtcggtgatg
     4801 atccttttgg ggcgttaatg caaagaacgc tgctaactga gggagtcgat atcacgtatc
     4861 tgaagcaaga tgaatggcac cggacatcca cggtgcttgt cgatctgaac gatcaagggg
     4921 aacgttcatt tacgtttatg gtccgcccca gtgccgatct ttttttagag acgacagact
     4981 tgccctgctg gcgacatggc gaatggttac atctctgttc aattgcgttg tctgccgagc
     5041 cttcgcgtac cagcgcattt actgcgatga cggcgatccg gcatgccgga ggttttgtca
     5101 gcttcgatcc taatattcgt gaagatctat ggcaagacga gcatttgctc cgcttgtgtt
     5161 tgcggcaggc gctacaactg gcggatgtcg tcaagctctc ggaagaagaa tggcgactta
     5221 tcagtggaaa aacacagaac gatcaggata tatgcgccct ggcaaaagag tatgagatcg
     5281 ccatgctgtt ggtgactaaa ggtgcagaag gggtggtggt ctgttatcga ggacaagttc
     5341 accattttgc tggaatgtct gtgaattgtg tcgatagcac gggggcggga gatgcgttcg
     5401 ttgccgggtt actcacaggt ctgtcctcta cgggattatc tacagatgag agagaaatgc
     5461 gacgaattat cgatctcgct caacgttgcg gagcgcttgc agtaacggcg aaaggggcaa
     5521 tgacagcgct gccatgtcga caagaactgg aatagtgaga agtaaacggc gaagtcgctc
     5581 ttatctctaa ataggacgtg aattttttaa cgacaggtag gtaattatgg cactgaatat
     5641 tccattcaga aatgcgtact atcgttttgc atccagttac tcatttctct tttttatttc
     5701 ctggtcgctg tggtggtcgt tatacgctat ttggctgaaa ggacatctag ggttgacagg
     5761 gacggaatta ggtacacttt attcggtcaa ccagtttacc agcattctat ttatgatgtt
     5821 ctacggcatc gttcaggata aactcggtct gaagaaaccg ctcatctggt gtatgagttt
     5881 catcctggtc ttgaccggac cgtttatgat ttacgtttat gaaccgttac tgcaaagcaa
     5941 tttttctgta ggtctaattc tgggggcgct cttttttggc ctggggtatc tggcgggatg
     6001 cggtttgctt gacagcttca ccgaaaaaat ggcgcgaaat tttcatttcg aatatggaac
     6061 agcgcgcgcc tggggatctt ttggctatgc tattggcgcg ttctttgccg gcatattttt
     6121 tagtatcagt ccccatatca acttctggtt ggtctcgcta tttggcgctg tatttatgat
     6181 gatcaacatg tgttttaaag ataaggatca ccagtgcgta gcggcggatg cgggaggggt
     6241 aaaaaaagag gattttatcg cagttttcaa ggatcgaaac ttctgggttt tcgtcatatt
     6301 tattgtgggg acgtggtctt tctataacat ttttgatcaa caactttttc ctgtctttta
     6361 tgcaggttta ttcgaatcac acgatgtagg aacgcgcctg tatggttatc tcaactcatt
     6421 ccaggtggta ctcgaagcgc tatgcatggc gattattcct ttctttgtga atcgggtagg
     6481 gccaaaaaat gcattactta tcggtgttgt gattatggcg ttgcgtatcc tttcctgcgc
     6541 gctgttcgtt aacccctgga ttatttcatt agtgaagctg ttacatgcta ttgaggttcc
     6601 actttgtgtc atatccgtct tcaaatacag cgtggcaaat tttgataagc gcctgtcgtc
     6661 gacgatcttt ctgattggtt ttcaaattgc cagttcgctt gggattgtgc tgctttcaac
     6721 gccgactggg atactctttg accacgcagg ctaccagaca gttttcttcg caatttcggg
     6781 tattgtctgc ctgatgttgc tatttggcat tttcttcctg agtaaaaaac gcgagcaaat
     6841 agttatggaa acgcctgtac cttcagcaat atagacgtaa acttttccgg ttgttgtcga
     6901 tatctcccta tccctcaacc ggaaaataat aatactaaag tgcttagccc tgctaataat
     6961 cacctaatcc aaacgcctca ttcatgttct ggtacagtcg ctcaaatgta cttcagatgc
     7021 gcggttcgct gatttccagg acattgtcgt cattcagcga cctgtcccgt gtatcacggt
     7081 cctgcgaatt catcaaggaa tgcattgcgg agtgaagtat cgagtcacgc catatttcgc
     7141 tatcaggatt ctgtgtgatg gttacatcgc ccggcccagg gctgtttagt catcagcgct
     7201 ttctgacagt gctgagattt caacctgttg cagtaaaaat gagtagatat aaggcaagtg
     7261 tgctgccaaa cctatctttt acggggtgaa ggtagatttc gtttgaaggg tatctggtgt
     7321 cccctgcaga catctactta gagtggcagg ggattgatta gaatggtatt ttttagatgt
     7381 gagaaatatt ttacccgcta ttttacccat tagcgcggct taagagctta tttttgaatt
     7441 cacaatggtc acgatataac catcttgctc gaccgtggat aactttggct ttaggcaggt
     7501 cgccggactt aatccggtcg tagatgaagg ttttaccaaa gccagtatca gccatgatga
     7561 atttcaaatc aaccagtgaa tcaggttgta gttcgtgttg catgagtgct atccgaatag
     7621 ggaatcgaac ctgcaaatca ggcaataaaa aaccgccatc aggcggcttg gtgttctttc
     7681 agttcttcaa ttcgaatatt ggttacgtct gcatgtgcta tctgcgccca tatcatccag
     7741 tggtcatagc agtcgttgat gtcctctgct tcgataactc tgttgaatgg ttctccattc
     7801 aattcacctg tgactcggaa gtgcatttgt gatttcccca aaagatgctt gagtgcgctt
     7861 cttattcgat tcgcacacct ggtatttcgc cttttgaaat ggctaagtca taaatttgcg
     7921 cagcactata cccatctcgc atccatgaat ctaaggcgcg aatagcctcg ctacgctttt
     7981 tatcttctct ttcatttttg atatcaacga ggacatcaac gcaattaagg catatgtgga
     8041 ttttgtcctt acattcgatc atggcagctt taccatgatt tccgccacac agtgagcata
     8101 aatcttcagg gtctggctgg tatttctgta acgttaaagg gttgaatgtt gaacaggcca
     8161 taatcatctc cataaaacaa aactcgccgt agcgagttca gataaaagaa atccccgtca
     8221 gtgcgaggat tgttattgtc ttttcttcgt gcatttgtcg cactttcggc accatccaga
     8281 taggcacatc cgtccgcaat taacacatat aggccacatc atttttcctc ttttggttta
     8341 tgaatctgaa tggtcatgcc gctttgagtg gtgactacaa tgacataacc aggctg
//
LOCUS       DAZFDP010000183         8366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFDP010000183 DAZFDP010000000
VERSION     DAZFDP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..259
                     /locus_tag="ACHJ1G_004406"
     CDS             <1..259
                     /locus_tag="ACHJ1G_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766144.1"
                     /translation="NIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRH
                     KMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            482..979
                     /gene="ubiC"
                     /locus_tag="ACHJ1G_004407"
     CDS             482..979
                     /gene="ubiC"
                     /locus_tag="ACHJ1G_004407"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4766145.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            992..1864
                     /gene="ubiA"
                     /locus_tag="ACHJ1G_004408"
     CDS             992..1864
                     /gene="ubiA"
                     /locus_tag="ACHJ1G_004408"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4766146.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2019..4442)
                     /gene="plsB"
                     /locus_tag="ACHJ1G_004409"
     CDS             complement(2019..4442)
                     /gene="plsB"
                     /locus_tag="ACHJ1G_004409"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4766147.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4613..4981
                     /gene="dgkA"
                     /locus_tag="ACHJ1G_004410"
     CDS             4613..4981
                     /gene="dgkA"
                     /locus_tag="ACHJ1G_004410"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4766148.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5091..5699
                     /gene="lexA"
                     /locus_tag="ACHJ1G_004411"
     CDS             5091..5699
                     /gene="lexA"
                     /locus_tag="ACHJ1G_004411"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4766149.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5772..7097
                     /gene="dinF"
                     /locus_tag="ACHJ1G_004412"
     CDS             5772..7097
                     /gene="dinF"
                     /locus_tag="ACHJ1G_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4766150.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7213..7422
                     /gene="yjbJ"
                     /locus_tag="ACHJ1G_004413"
     CDS             7213..7422
                     /gene="yjbJ"
                     /locus_tag="ACHJ1G_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4766151.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7464..7979)
                     /gene="zur"
                     /locus_tag="ACHJ1G_004414"
     CDS             complement(7464..7979)
                     /gene="zur"
                     /locus_tag="ACHJ1G_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4766152.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8297..>8366
                     /gene="yjbL"
                     /locus_tag="ACHJ1G_004415"
     CDS             8297..>8366
                     /gene="yjbL"
                     /locus_tag="ACHJ1G_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO4766153.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDN"
BASE COUNT         1916 a         2023 c         2277 g         2150 t
ORIGIN      
        1 taatatcttt atcctgttct cctctgccga tgatgggcca gttatggtgg taagctccca
       61 gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta
      121 taaatccaga cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa
      181 atctcatggt aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat
      241 aaagaatata ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga
      301 tgcccctacg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcggt
      361 ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac
      421 atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg
      481 catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc
      541 cctggatccg caactgctcg actggctgtt gctggaggat tccatgacaa aacgttttga
      601 acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga
      661 aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt
      721 gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt
      781 aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt
      841 cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg
      901 gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc
      961 ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct
     1021 gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg
     1081 gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct ggatcctggc
     1141 ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga tgtgtggtga atgattatgc
     1201 tgaccgaaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc
     1261 ggtaacagag aaagaggcgc gcgcactgtt tgtagtgctg gtactgattt cgtttttact
     1321 ggtactgacg ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg
     1381 ggtgtacccg tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt
     1441 tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg
     1501 gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt
     1561 tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt tcggccaata
     1621 cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg
     1681 tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt
     1741 tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat
     1801 gaataataac tatgttggtc tggtactatt tttagggctg gcaatgagtt actggcattt
     1861 ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgcgg
     1921 cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg
     1981 gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca
     2041 ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc
     2101 atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc acgcagtgtc
     2161 agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc
     2221 acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac
     2281 gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct
     2341 gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc atcatcttgc
     2401 agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac
     2461 tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg
     2521 tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc
     2581 gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc
     2641 tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt
     2701 tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag
     2761 taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg
     2821 gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc
     2881 gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt
     2941 aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc
     3001 tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga
     3061 ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg
     3121 ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc
     3181 ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc
     3241 gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac
     3301 tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa
     3361 agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc
     3421 ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg
     3481 tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac
     3541 accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg
     3601 ccctgataaa gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg
     3661 taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca
     3721 tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg
     3781 agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc agccagacgt
     3841 tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gagcgatagt tttatctgtg
     3901 ccgtgttcat cggccatgcg gcgcagagaa actgacggcg agaaacgcac aaaactgtcg
     3961 cgaccgagcc acagtacggc gaaaaatttt tgtacgccgt taagcatacg cagcggcggg
     4021 tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc
     4081 tgcacatcca gatttgggtt gctgcggtgc aaatcaagat agtcgtggaa cagcttaata
     4141 gactcttctt tcggcgtgta ataggtgaac acacgcggcc cgccgtgaat gaacacatag
     4201 cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa gtcatgagcc
     4261 agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata
     4321 attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt
     4381 tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac
     4441 ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc
     4501 atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc
     4561 ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa
     4621 taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg
     4681 cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt
     4741 ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt
     4801 gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat
     4861 tggctctgaa taccatgagc tttccggacg cgcaaaagat atgggatccg ccgcggtgct
     4921 gattgccatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata
     4981 acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc
     5041 ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt
     5101 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
     5161 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
     5221 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
     5281 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
     5341 ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct
     5401 tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg
     5461 gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg
     5521 tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata
     5581 aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc
     5641 agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac
     5701 atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca
     5761 ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac
     5821 ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg
     5881 gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct
     5941 ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt
     6001 atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt
     6061 tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gcgctgcata
     6121 ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa atccgctggt
     6181 taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat
     6241 atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg
     6301 tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg acggttattg
     6361 cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg
     6421 gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc
     6481 ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgataaccg
     6541 tacttggtgc gcgactgggg agtgacattg tcgctgttaa cgcggttctg atgacgctac
     6601 tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg cactccggtc
     6661 aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca gcgtgccgcc
     6721 agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca
     6781 ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc
     6841 aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg
     6901 caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga
     6961 cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc
     7021 tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc aacggtacct
     7081 ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc aaattgctga
     7141 ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat
     7201 gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa
     7261 gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt
     7321 gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa
     7381 gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga
     7441 cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca
     7501 tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca
     7561 tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata
     7621 atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcaaataaac
     7681 atggctgacg tatgggtggg ctggtcgaac agatgacaga gcacataact gttggtggat
     7741 tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc
     7801 ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg
     7861 ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta
     7921 cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc
     7981 gggcacctca aagtgggggg taagaatgtc cttactttac catgttccag gaaaaacacc
     8041 gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttgtgttg
     8101 ccttgtcgta ccattatcaa cacgataata attaataatt catttttaaa taaattgctt
     8161 caagttaact cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat
     8221 gagcgatttt tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa
     8281 aaaaggtaga tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac
     8341 actcaattca aatcaattcg ataatg
//
LOCUS       DAZFDP010000184         8270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFDP010000184 DAZFDP010000000
VERSION     DAZFDP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..372
                     /locus_tag="ACHJ1G_004416"
     CDS             <1..372
                     /locus_tag="ACHJ1G_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4766154.1"
                     /translation="LLEQKAHQLNQQLVAAAPKVDFADRVSVAKGILIGNFAKVVGLK
                     QNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTGK
                     GQQWLTRKLLDAGLLKPVAAE"
     gene            complement(448..1467)
                     /locus_tag="ACHJ1G_004417"
     CDS             complement(448..1467)
                     /locus_tag="ACHJ1G_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HFO4766155.1"
                     /translation="MADVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKAMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYSKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFN"
     gene            complement(1460..3169)
                     /locus_tag="ACHJ1G_004418"
     CDS             complement(1460..3169)
                     /locus_tag="ACHJ1G_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766156.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     NNG"
     gene            complement(3770..4411)
                     /locus_tag="ACHJ1G_004419"
     CDS             complement(3770..4411)
                     /locus_tag="ACHJ1G_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HFO4766157.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLRPLINIIHGTQTLAQKQVPQGELTGKGNYL"
     gene            complement(4600..5160)
                     /locus_tag="ACHJ1G_004420"
     CDS             complement(4600..5160)
                     /locus_tag="ACHJ1G_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HFO4766158.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISNEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANILH"
     gene            complement(5406..5717)
                     /locus_tag="ACHJ1G_004421"
     CDS             complement(5406..5717)
                     /locus_tag="ACHJ1G_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HFO4766159.1"
                     /translation="MRKDFKIDGKYVVLSVSSQIQSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMAEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(5768..6799)
                     /locus_tag="ACHJ1G_004422"
     CDS             complement(5768..6799)
                     /locus_tag="ACHJ1G_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4766160.1"
                     /translation="MTNSLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSDHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(6796..7191)
                     /locus_tag="ACHJ1G_004423"
     CDS             complement(6796..7191)
                     /locus_tag="ACHJ1G_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766161.1"
                     /translation="MFNGLTDAKVNALLNDAKQKAANPGTNENERAMAVAIWELAEEV
                     KRNRLLYTRVYQQFDGASWIDIEEAEYATCKKEKKIVRILHKAATPITVEIPDHRYFE
                     NDDLPGDVIRIIDEILHEQGLTVKITHNK"
     gene            complement(7211..7432)
                     /locus_tag="ACHJ1G_004424"
     CDS             complement(7211..7432)
                     /locus_tag="ACHJ1G_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HFO4766162.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKWFERDSYQLSLSRGEEKIER
                     TRGRWRFYDENHNQVKAEPILYTLLKTII"
     gene            8037..8246
                     /locus_tag="ACHJ1G_004425"
     CDS             8037..8246
                     /locus_tag="ACHJ1G_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HFO4766163.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
BASE COUNT         2005 a         2062 c         1947 g         2256 t
ORIGIN      
        1 cttctggaac agaaagccca ccagctaaat cagcagctgg tggccgccgc tcctaaggtc
       61 gattttgccg accgggtatc agtggccaag gggatcctga ttggaaattt tgcaaaggtt
      121 gttgggctta agcaaaacgc gttgtttgcc tggttacggg agaacggcat cctgatagcg
      181 tccggtggac gtaaaaatgt gccgttccag caatacatca acgccgggta tttcacggtg
      241 aaagaagtgg tgctggatga tgaagatggt taccagatac ggctgacgcc ccaattaacg
      301 ggtaaaggcc agcagtggtt aactcgcaag ctacttgatg ctggtttgtt aaaaccagta
      361 gcggctgagt gattaaatta aaaaggcggc ctttcggccg ccagtgattt cagatacccc
      421 ggcagccgcc ggggttattt ttggtggtca attaaacgga ttgattgaat tattaaacgt
      481 gatgatgctt gtctcacgcg gtgcctggac gttagccgct tgcggaacct ccttaatttt
      541 cttggtgaca ggcaagttgc gtgcgccaac tttgatcaga gattcgaaaa gtgtcgcaac
      601 gatttttgca tcaccaggtt ctttgaggcg gaatgcgtct ttttgggcgg cggagacgaa
      661 gatcgggagg ttatccagtt cgtcttgcat tgctgccagc acatcgtcgc ggatacccgc
      721 tgtttcctcc agcaaagcaa ttcgcgcttc agcatctgcg atcttggcca ttgcttcaag
      781 gtggcgaccc tggctttcga gtagtgcggt ttccagttca gccgtacgct ctgtcgcctc
      841 caccatcatt tccagttcag ccattttact gtaatgggat ataacggcct gcactgactc
      901 gtcggagtac ccatgcgccg ccagggactc tgccagtaaa gatttagaat ccgcgctttc
      961 aaacattccg gcgctggcag gatgatccag actgatatag ttcggcgttg tcacataatc
     1021 cacaccatgg aagctggtgg ttacagcgat tttcccggac tcacgcccgc cagtggccca
     1081 gctccagcca ccagcacggc tttcgatcat agcggcgaca attttacccg gctctgtgtt
     1141 aagaatttcc tgtgtatggg taacgatgcc gttgtcgtca acagatatag ccactgttcg
     1201 gcacgctgga acattgtcga ttacgaccgg gcgaccttcc accatgatca cgctggtttc
     1261 tggtacttcc agtttgccgg tcagctgtcg gcgaccgtga ccgtaatagc cgaaaagctc
     1321 accaaggcgt aaaccttcct gagtttcctt gctttcaagc atggccttta ccgcgcttaa
     1381 tacatactgt cgcccgttct ggcgaccttt tcgagcattg ctatagagac aaaagcggtc
     1441 agtgaccgtt ttcaaaacat cagccattat tgtttccctc tttaaagacc gattcaagga
     1501 tttgcgccag ttcctgtggc ggtgttttga tgatggaatc catcaggtga tcgtcgtcct
     1561 cgcttttagc tttcagttcg ttcaccaggg cttcagagat tttttcgtca atctccagca
     1621 catcgctgaa caggtaacgt ttgaatgcat cggaattggc gaggacgctg ttattgctga
     1681 cggcatcgag gatttgcgta acgatggtgg cgtagttcgc ctgcgagtcg cggttatcgt
     1741 tgtgctcttg ttgcagagcg gtattaacgg agtggaattc gattttgtac gggcgatcac
     1801 cttccgggta taccttgccg tacttgaaag caagatgaat atcgatagcc cgctgaatga
     1861 actcttctac gccctgctgg atccatgagg cgcgcatggc ggcctgaatc gccgtgcgca
     1921 ggaatccacc ttcaccaagc ccgccggaca tttgatctgc ccaccccagg agggtgtaat
     1981 cgaggccaag tgctgccgcc agctggcgca tataagtgag aatgtcttca atgccgttga
     2041 tgtctgcctg gatggtctga gtatcaatgg tcatctgccc cttgccgtcg cccataatag
     2101 gcagcagggt attggtgacc gtaggcatgt tattcgcgcc gcgtgcgcgc ttttccatca
     2161 ggtcagctgc tcgtttaagc gtctgagtaa tggtgcgtga ataatcggct gcttttaccg
     2221 gatccagact attcatcgcc agaccgatga ttcggtcaat tttcgacgca ttaaaacgcg
     2281 ttgccttcag cgagcggatc gccgaacgca ggttcatgta cggctcgtag gcgtattcga
     2341 gcaagctggt cccgtaattc tgggtttcaa tcggcgtgcg ctcttccgga ttatccagca
     2401 ggctgtaagc cttatggcca gtgtgcacag gcataaggtt tgacttaggc cgccagtagg
     2461 ggattttcat agggataatg gtccacggat cggcgaaaac cattttccct gacgcgtcct
     2521 tcagataatc gccgctaaat cccgccaggt taccgctgac ctcgaactcc ttgatgaagc
     2581 tcggaagggt gtaataggag cactcaaaag acgtgatccc tatgccttct ttggcgtatg
     2641 gcctgacata agccacccca aatacagaca tgataaatgc ccacccggcg acctctttgt
     2701 tgatggttcg cccgatgtcg ttcatcagct cgtcacacaa cccctgcgcg gcgtcatagt
     2761 cactatcgtt tccgttgtgt accggcacga tagagaaggt ttgtccggtc ttcttatcga
     2821 aagagagcgc gtgcgtaata tggatgttca gcgcggtggc gatcgtgctg taaaccgcca
     2881 tctcttcgag tagcggatag cgttgcaagc ggtcttccgg cagttgaact tcatcaaaga
     2941 taaagcgact tccgtccacc agcccatcgc cagccatgcc actatcgccc ggtttgccgc
     3001 ctaagaagcc ggacagttgt accggtgccc ctgcgcgaga aaacaaatac ccacttccgc
     3061 cgtgcacagc cagcgcggac aggaggatgt tgtcccgttc tccgttgtct ttaaaaaccc
     3121 ccgccagcgc cttcctgacc gaggatagcg tgattttatt gtctgccaag attgcacctt
     3181 aattagaata attcgcatcg tgtttgaacg gaatttaaca ctagtcactt gttaaggatt
     3241 accaatgaac aagctatcta tgggggtgtt tcgctgttca agtgtcagcg aaatattgaa
     3301 atatattagt gcaataacat ttcatcgggc gccgattaga tacggcgtgg aaaaggtgga
     3361 aggcaaaagc tatgaccgac tacgccggac gtgaaataat atgtccacac tatctgatac
     3421 aataaaaccg aataggacat aatttcgacg tcaaagcctt ggcacaggct ggagagaaaa
     3481 cttgaaaaag cgatactaca cagtaaagca tgggacgcta cgagcattac aagagtttgc
     3541 tgacaagcat aacgttgagg tgcgaaggga agggggcagt aaagctctgc gcatgtaccg
     3601 tccggacggg aaatggcgga cagtcgttga tttcaaaaca aacagtgttc cccagggggt
     3661 ccgcgatcgg gcattcgaag aatgggagca gatcatcata gataacgcat tgctcctgaa
     3721 tgcagattaa actttccgcc cttttgcccg gtctatcgcc gggcatcttt cagagataat
     3781 tacctttacc cgtcaattcc ccttgtggta cttgtttttg cgccagtgtt tgggttccgt
     3841 gaattatgtt aatcagaggt cttagtaacg atggttcctg gcgggcctca acttctccag
     3901 ccattgccct gatgtagtcg gcgctggcaa cattgttgta ttccgttgcg aaacagcata
     3961 acaatgtcag aacatgctct gttgttattt cgctccagtt gatgttgaaa aattcatcgc
     4021 cttttttatc gtgttcggaa tcgaagatgc tttggtggag gatgtatttg ccggattcct
     4081 tgcgcggtaa cttgatcgct ttctggcgtt ccagctcctt gtaaatctgc attgcttcaa
     4141 ttagtaccgg cctgccgttc atgaagggat cgcgcaacct tacacgctgg ccaactcgac
     4201 cggtaataaa gctgttttcc tcttccacca gcacgataaa acccttttcc tctttttctc
     4261 gcaattcgcg cagcagctgg agttccatat cgcggcggcg ttcagggtag ctggtccgct
     4321 cagccatgat cagctcattg ttgatccatg cagcagtcat tgacgccggt ttgccgacgc
     4381 tcattgaaac aacgcatatt ttcttatcca tagcgcccct acaaaaaaga aaagccacca
     4441 gcggcggctt agcaatacaa ctgaaggtag cgcccggtac tcagactgtg ccgtccatgg
     4501 aatatttgaa aagggatcca tccgtaccgg gcgtgtgatg attctgactg aagtcacttg
     4561 tcagttgtca atcatttatc attaaaaata atatatttat tagtgcaaga tgtttgccat
     4621 ctcataggca tcagccagta actccatctc tgacttgttc agcaaggtga attctttctt
     4681 gcctccaacc acaccatcgg catgaacagg gaccagccat gggtattttt ctcttacttc
     4741 agccggagct gcatgctggt ggtgccatct acaaagtggc aattgctttt tatgacaacc
     4801 cggcgcggta cgaccggcga tatggtgcag agacacctca ttagatatta ctccatgcat
     4861 atagcaggca atgcagggga gagcgccaag agcattggcg atgcgccgtt cctccgccgt
     4921 tggtgttctc cccttcaagc cacgagattt tatttttacc gcgcttttcc gcgttttgct
     4981 ggctggtggg cgctctttct gtttagcgat acggcggtcg atagaatccc gcattttctg
     5041 atattgcgat tctctccagg cggggtcagc caacttttcc cgttgccgag cgatcgctcg
     5101 ttctctggct gccttctgcc actcgcggcg ctgttcaagt ttttgttcga ttgttttcat
     5161 atggcaaaaa aaaggcggcc taatggccgc caatgatgtc aaggagttaa gtaatggcaa
     5221 cgtcttcgta gttgacaaaa actgcggctc aattatagca atcaattaga gcaatggtag
     5281 atattttgtt aatcgagaat cacatttttt cacttcagta cctgtgtgct atactctttc
     5341 ttgattgatt ggatgcggaa tacaaacccg ctcttttgtg cagcctggct ccttgccagg
     5401 cttttttatt tcatcatgga agctgttaac gctttggatc ttgctgaact gattgaaagg
     5461 gcattgttta ccttacccag gagttcgcca aattccgcca tcactctagt aagccctcgc
     5521 cgcgcttcct cctccgttgc attcatcaca aaatgttcag cactccgcat gcttttaacg
     5581 gggaacgcaa cagatatcga gtcgatatca ggcatcctat cgctcaattt tacggtgaca
     5641 atgacagatg gtgactgaat ttgagagctt acagacagca ccacatattt tccgtctatt
     5701 ttgaaatcct tccgcatgtg tcaccataaa tatcaaataa ttagagcaat caagtaaaaa
     5761 tgaacggcta atcgccatct tccagcaggc gcaccattgc ccccgtttca ctatccaggt
     5821 tacgaatgta gttcatgaca atatttacgt tggtccagcc accagcttgc atgatctccg
     5881 gtattgaaac tccggcgcgg gccatatctc gcgcggcacc gacacgggca ctatgtccag
     5941 accaggccag gtatcgctga ccagagtcat cttttgcccc gtaaatcaat cggtgagttg
     6001 cttcaaaaat cccttccagg gcgcgagttg atagctggct ggtggatgat ggcgcggcaa
     6061 caccattttt tctgacgcgg caaaacaggt agttattcgg atcatcagct acaccagaga
     6121 cagaaatcca tcgctcaacc agtttagtta cccccaggct aagtgccttc tctacaccag
     6181 cggtgctaac cagcgttttc gttctgccaa tatggattaa cattctccca ccgtcagtac
     6241 gtgagatatc tttaaccctg atcctagaaa tttcggctat acgtaacagg gtgttataag
     6301 caatccccag aaatgccaga ttacgtatat cctggcagcg atcgctattt tccatgagtg
     6361 aacgaacctg gtcgaaatca gtgcgttcga acgccagcgc ctgttttgca cgctcaccgg
     6421 catcaacgtt ttcttttcgg atccgtcgca tgaccagtga aacagcattg ctgtcacttg
     6481 gtcgtggcag cccggaccga cgatgaagca tgtttagctg gcccaaatgt tgctggatag
     6541 tttttactgc cagaccacgc gcctgaagat atagaaggta atcgcgaaca tcttcaggtt
     6601 ctgcgggaaa ccatttccgg ttattcaact tgcaccatgc cgcccacgac cggcaaacgg
     6661 acagaagcat tttccaggta tgatcagaaa acgcctggcg atccctgaac atgtccatca
     6721 ggttcttgcg aacctcatca ctcgttgcat cgaccggcaa tgcaggcaaa ttttggtgaa
     6781 cggttaacga attagtcatt tattatgcgt gattttaaca gttaagcctt gttcatgaag
     6841 tatctcatca ataattcgaa tcacatcccc tggtagatca tcgttctcga aatatctgtg
     6901 atcggggatt tctacagtga ttggggttgc tgctttatgc aaaatccgaa caatcttttt
     6961 ttcttttttg catgttgcat attcagcctc ttcgatatct atccagctcg caccatcaaa
     7021 ttgctggtaa acccgagtgt atagtaagcg gtttcgcttt acttcttctg ctaactccca
     7081 aattgcaaca gccatagcac gttcattttc attcgttcca gggttagcag ctttttgttt
     7141 tgcgtcgttc agtaaggcgt ttaccttggc atctgtcagg ccattaaaca ttcatcacct
     7201 catgatcaag ttaaattatt gttttcagca gagtgtacag gattggctct gcctttacct
     7261 ggttatggtt ctcgtcatag aaacgccagc gaccgcgcgt gcgttctatt ttctcttcac
     7321 cgcgagataa tgacagttgg taactatcac gctcaaacca ttttgcccgc cagtaaccac
     7381 ggtttttctc aagctcaaga tgagtggaca ctttagcagc tgaatatccc atttttcacc
     7441 tctgattgat tggtggtgct aagtgcgcta cgcgaaatct ggagcactaa cactgccaac
     7501 atttcgcaga ttttacgtag cgcaaccttg atcaaatgat caagtgatca ctatttgacc
     7561 tgataaggta ttgaactgta tggatttaca ggtaaattaa tcatgttcaa taacccttaa
     7621 gataacttcg tataatgtat gctatacgaa gttatcagat ccgaagagga gtttacgtcc
     7681 agctgcgcac aaaaatcaag aattattaga gcaataaatt tagagagaaa aatcccactc
     7741 caccagctaa aaactggatt gtttttcata gttgtttgac aattgctcta ataaattata
     7801 gttttgccgt cgttacgtaa tacgactttg gattcactat ttaatgtgtc ttcagcgttg
     7861 tagagcggct cagaaggaaa tgagcaaaca gggaaacctt atacaacggc attacagcta
     7921 tgcattgctc atcttacaca cagcgcaatg ttgttagatt accccagcat ggatcatggg
     7981 tgaaacagta ggtcagagct tcaggctctg tgttgtcaat acagtgaggc ataattatgg
     8041 ctttcattca accaaccatc gacgacgtta gacattgctc taacgcttta tctgtagacc
     8101 ctgccgaaac cgacgctgcc cgcgccattg ctgaacacta ctcaaagata tccaatcagg
     8161 agtaccgcat cacccaagac gacctggatg acctcactga cacaatcgaa tatctcatgg
     8221 caactaacca gttagactca caataaatgc actaataaat ctattatttt
//
LOCUS       DAZFDP010000185         8126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFDP010000185 DAZFDP010000000
VERSION     DAZFDP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            551..2119
                     /gene="cydA"
                     /locus_tag="ACHJ1G_004426"
     CDS             551..2119
                     /gene="cydA"
                     /locus_tag="ACHJ1G_004426"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4766164.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2135..3274
                     /gene="cydB"
                     /locus_tag="ACHJ1G_004427"
     CDS             2135..3274
                     /gene="cydB"
                     /locus_tag="ACHJ1G_004427"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4766165.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3289..3402
                     /gene="cydX"
                     /locus_tag="ACHJ1G_004428"
     CDS             3289..3402
                     /gene="cydX"
                     /locus_tag="ACHJ1G_004428"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4766166.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3402..3695
                     /gene="ybgE"
                     /locus_tag="ACHJ1G_004429"
     CDS             3402..3695
                     /gene="ybgE"
                     /locus_tag="ACHJ1G_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4766167.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3845..4249
                     /gene="ybgC"
                     /locus_tag="ACHJ1G_004430"
     CDS             3845..4249
                     /gene="ybgC"
                     /locus_tag="ACHJ1G_004430"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4766168.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4246..4938
                     /gene="tolQ"
                     /locus_tag="ACHJ1G_004431"
     CDS             4246..4938
                     /gene="tolQ"
                     /locus_tag="ACHJ1G_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4766169.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4942..5370
                     /gene="tolR"
                     /locus_tag="ACHJ1G_004432"
     CDS             4942..5370
                     /gene="tolR"
                     /locus_tag="ACHJ1G_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4766170.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5435..6700
                     /gene="tolA"
                     /locus_tag="ACHJ1G_004433"
     CDS             5435..6700
                     /gene="tolA"
                     /locus_tag="ACHJ1G_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4766171.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6833..8125
                     /gene="tolB"
                     /locus_tag="ACHJ1G_004434"
     CDS             6833..8125
                     /gene="tolB"
                     /locus_tag="ACHJ1G_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4766172.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
BASE COUNT         1937 a         1921 c         2210 g         2058 t
ORIGIN      
        1 ctttattagt aatcctgaaa ctctgcgtcg tattagccag tgaccaaaaa aagaattaag
       61 gtcaaccgtg ctgtttttgc ttcgtctctt tttatcttta attgccaacc gaaactaatt
      121 tcagccttat aactcacaca ttttaaacat aaatgtcact aaagttacct tattgaaaca
      181 tgattaacat aatttgtagg aattgatatt tatcaatgta taagtcttgg aaatgggcat
      241 caaaaagaga taaattgttc tcgatcaaat tggctgaaag gcggtaattt agctataaat
      301 tgatcaccgt cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg taaggatttt
      361 gcggcgtaat atatacgtgg gatcaatttg agtttttatt aacatgtttg caacctttct
      421 ttacgccgtt tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca aatttctatt
      481 ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca
      541 aggagtcatg atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat
      601 gtaccacttc ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga
      661 aacggtctac gtcctctccg gcaaacagat ttataaagat atgaccaagt tctggggcaa
      721 gttgtttggt atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg agttccagtt
      781 cgggactaac tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc
      841 aatcgaaggt ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg
      901 ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc
      961 aaacctgtcc gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc
     1021 cgatttcaac tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa
     1081 cccggttgct caggtgaaat tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat
     1141 gttcatcctc ggtatcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa
     1201 acgctccttt gctatcgctg ccagcttcgg tatggctgct gttctgtctg ttattgttct
     1261 gggtgatgaa tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga
     1321 agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca
     1381 ggaagaggag acgaacaaat ttgcgattca gatcccttac gcactgggca tcattgcaac
     1441 gcgttccgtg gataccccgg ttatcggcct gaaagagctg atggtgcagc atgaagaacg
     1501 cattcgtaac gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga
     1561 ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac ctcggttacg gtctgctgct
     1621 gaaacgctat acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa
     1681 agactccatc ccgcgtgtag cgccgctgta ctttgcgttc cgtatcatgg tggcgtgtgg
     1741 cttcctgctt ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg
     1801 cgagaaaaaa tggcttctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt
     1861 agaagcgggc tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct
     1921 gccgacagct gtggcgaact cgtcactgac cgcaggcgat ctcatcttct caatggtgct
     1981 gatttgcggc ctgtataccc tgttcctggt ggcagaattg ttcttaatgt tcaaatttgc
     2041 acgcctcggc ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt cttccacgac
     2101 tactcagccg gcacgctaag acaggagtcg tcaaatgatc gattatgaag tattgcgttt
     2161 tatctggtgg ctgctggttg gcgttctgct gattggtttt gcagtcactg acggtttcga
     2221 catgggggtg ggcatgctca cccgtttcct cggtcgtaac gacaccgagc gtcgaattat
     2281 gattaactcc attgcaccac actgggacgg taaccaggtt tggctgatca ccgcgggcgg
     2341 cgcactcttt gctgcctggc cgatggtcta tgccgctgcg ttctccggct tctatgtggc
     2401 gatgatcctc gtgctggcgt ctttgttctt ccgtccggtc ggttttgact accgctccaa
     2461 gattgaagaa acccgctggc gtaacatgtg ggactggggc atcttcattg gtagcttcgt
     2521 tccgccgctg gtaattggtg tagcgttcgg taacctgttg cagggcgtac cgttcaacgt
     2581 tgatgaatat ctgcgtctgt actacaccgg taacttcttc cagttgctta acccgttcgg
     2641 cctgctggca ggcgtggtga gcgtagggat gatcattact cagggcgcaa cctatctgca
     2701 aatgcgtacc gtgggcgaac tgcacctgcg tacccgtgca acggctcagg tggctgcgct
     2761 ggtgacactg gtctgtttcg cactggctgg cgtatgggtg atgtacggta tcgatggtta
     2821 tgtcgtgaaa tcgacaatgg accattacgc agcctctaac ccactgaata aagaagtggt
     2881 tcgtgaagct ggcgcatggc tggttaactt caacaacacg ccaattctgt gggctattcc
     2941 ggcactgggt gtggttctgc cgctgctgac catcctgact gcacgtatgg ataaagccgc
     3001 gtgggcgttt gtgttctcct ccctgacgct ggcctgcatc atcctgacag ccggtatcgc
     3061 aatgttcccg tttgtgatgc cgtccagcac catgatgaac gcaagtctga caatgtggga
     3121 tgcaacttcc agccagctga cgcttaacgt catgacctgg gttgcggtgg ttctggtacc
     3181 gatcattctg ctctacaccg cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga
     3241 agatattgaa cgtaacaccc actctctgta ctaagtaagg agctaaaaat gtggtatttc
     3301 gcatggattc tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt
     3361 gaacacgtcg aatcaggcaa agccggtcaa gaagacatct gatgagtaag attatcgcga
     3421 ctttgtatgc ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc
     3481 tgttagcagg atgtatgttt tgggacccat ctcgttttgc cgcgaagacc agtgaactgg
     3541 aaatctggca tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg
     3601 gctttcgtcc gcagaaggtt ctttggcaag ggattttttg cccattgctt gccgatattg
     3661 ttctcattgt cgggctgatt ttcttcttct tttaaatcag aattctcttt aaaaaattat
     3721 gggccgctcc aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc
     3781 aatcgcgcgc gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg
     3841 taaagtgaat acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc
     3901 cggtggtgtg gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat
     3961 gctgcgtcat catcacttca gtcagcaggc gctgatggct gaacgcgttg cctttgtggt
     4021 acgtaaaatg acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca
     4081 gactgaaata acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc
     4141 cgagaatact ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat
     4201 gaagcctcgt gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat
     4261 atccttgatt tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt
     4321 ggtttttcaa tcgcatcctg ggccattatt atccagcgga cccgtattct taacgcagcg
     4381 gcgcgcgaag ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc
     4441 tatcaagaga gtcaggggaa acgggataat ctgactggtt cggaacaaat attttacagc
     4501 gggttcaaag agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg
     4561 gaaggggcgt cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg
     4621 cacattccgt tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg
     4681 gtctggggga tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa
     4741 atggttgcgc ccggtatcgc agaagcgttg attgcgactg caattggtct gtttgccgct
     4801 atcccggcag ttatggccta caaccgcctc aaccagcgcg taaacaaact ggaactgaat
     4861 tacgacaact ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc
     4921 gagagcaata aggggtaagt catggccaga gcgcgtggac gaggtcgtcg cgaacttaag
     4981 tccgaaatca acattgtacc gttgctggac gtactgctgg tgctgttgct gatcttcatg
     5041 gcgacagcgc ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc tactgaatca
     5101 caggcggtga gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag
     5161 tacaccgtgg tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg
     5221 gaagtgtcca gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa
     5281 gatgtgcctt acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa
     5341 tcggttggtt taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag
     5401 cgggtaacag gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga
     5461 caagctcaag cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct
     5521 gatctggagt tcgttcgatg agaatataga agcttcagcc ggaggcggcg gtggttcgtc
     5581 catcgacgct gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag
     5641 ccaggaatca agcgcgaagc gttctgatga acagcgcaag atgaaggaac agcaggctgc
     5701 tgaagaactg cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga
     5761 gcggttagcg gctcaggagc agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt
     5821 aaagcagaag caagctgaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc
     5881 cgaagcagat gctaaagctg cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa
     5941 aaaagcagaa gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc
     6001 cgctgcggca ctgaagaaga aagcggaagc ggcagaagca gctgcagctg aagcaagaaa
     6061 gaaagcggca actgaagctg ctgaaaaagc caaagcagaa gctgagaaga aagcggctgc
     6121 tgaaaaggct gcagctgata agaaagcggc agcagagaaa gctgcagccg acaaaaaagc
     6181 agcagaaaaa gcggctgctg aaaaggcagc agctgataag aaagcagcgg cagaaaaagc
     6241 cgccgcagac aaaaaagcgg cagcggcaaa agctgcagct gaaaaagccg ctgcagcaaa
     6301 agcggccgca gaggcagatg atattttcgg tgagctaagc tctggtaaga atgcaccgaa
     6361 aacgggggga ggggcgaaag ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa
     6421 caatggcgca tcaggggccg atatcaataa ctatgccggg cagattaaat ctgctatcga
     6481 aagtaagttc tatgacgcat cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact
     6541 ggcacccgat ggtatgttac tggatatcaa acctgaaggt ggcgatcccg cactttgtca
     6601 ggctgcgttg gcagcagcta aacttgcgaa gatcccgaaa ccaccaagcc aggcagtata
     6661 tgaagtgttc aaaaacgcgc cattggactt caaaccgtaa tcgcgatgtt gactgttcgg
     6721 acggtcaaca tcaggcaccg gttgccacgg ggttctggta gttttgtgta ttttagtttg
     6781 ttaacattct gctaaattat cgtgggccat cggtccagat aagggagata tgatgaagca
     6841 ggcattacga gtagcatttg gttttctcat actgtgggca tcagttctgc atgctgaagt
     6901 ccgcattgtg atcgacagcg gtgtagattc cggtcgtcct attggtgttg ttcctttcca
     6961 gtgggcgggg cctggtgcgg cacctgaaga tattggcggc atcgttgctg ctgacttgcg
     7021 taacagcggt aaatttaatc cgttagatcg cgctcgtctg ccacagcagc cgggtagtgc
     7081 gcaggaagta caaccagctg catggtccgc actgggcatt gacgctgtag ttgtcggtca
     7141 ggtcactccg aatccggatg gttcttacaa tgttgcttat caacttgttg acactggcgg
     7201 cgcaccgggt actgtacttg ctcagaactc gtacaaagtg aacaagcagt ggctgcgtta
     7261 tgctggtcat accgccagtg atgaagtgtt tgaaaaactg accggcatta aaggtgcgtt
     7321 ccgtactcgt attgcctacg ttgttcagac caacggcggt cagttcccgt atgaactgcg
     7381 cgtatctgac tatgacggtt acaaccagtt tgtcgttcac cgttcaccgc agccgctgat
     7441 gtctccggcg tggtcaccag acggttctaa actggcttat gtgaccttcg aaagcggtcg
     7501 ttccgcgctg gttattcaga cgctggcaaa tggcgctgta cgtcaggtgg cttcattccc
     7561 gcgtcacaac ggtgcacctg cattctcgcc agacggcagc aaactggcat tcgccttgtc
     7621 gaaaaccggt agtctgaacc tgtacgtaat ggatttggct tctggtcaga tccgccaggt
     7681 gactgatggt cgcagtaaca ataccgaacc gacctggttc ccggatagcc agaacctggc
     7741 atttacttct gaccaggccg gtcgtccgca ggtttataaa gtgaatatca acggcggtgc
     7801 gccacaacgt attacctggg aaggttcgca gaaccaggat gcggatgtca gcagcgacgg
     7861 taaatttatg gtaatggtca gctccaatgg tgggcagcag cacattgcca aacaagatct
     7921 ggcaacggga ggcgtacaag ttctgtcgtc cacgttcctg gatgaaacgc caagtctggc
     7981 acctaacggc actatggtaa tctacagctc ttctcagggg atgggatccg tgctgaattt
     8041 ggtttctaca gatgggcgtt tcaaagcgcg tcttccggca actgatggac aggtcaaatt
     8101 ccctgcctgg tcgccgtatc tgtgat
//
LOCUS       DAZFDP010000186         8125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFDP010000186 DAZFDP010000000
VERSION     DAZFDP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(355..504)
                     /gene="ydfW"
                     /locus_tag="ACHJ1G_004435"
     CDS             complement(355..504)
                     /gene="ydfW"
                     /locus_tag="ACHJ1G_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO4766173.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(577..1695)
                     /locus_tag="ACHJ1G_004436"
                     /pseudo
     CDS             complement(577..1695)
                     /locus_tag="ACHJ1G_004436"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            complement(1729..2148)
                     /locus_tag="ACHJ1G_004437"
     CDS             complement(1729..2148)
                     /locus_tag="ACHJ1G_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4766174.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            complement(2189..3154)
                     /locus_tag="ACHJ1G_004438"
     CDS             complement(2189..3154)
                     /locus_tag="ACHJ1G_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766175.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(3135..3656)
                     /locus_tag="ACHJ1G_004439"
     CDS             complement(3135..3656)
                     /locus_tag="ACHJ1G_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4766176.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(3640..3867)
                     /locus_tag="ACHJ1G_004440"
     CDS             complement(3640..3867)
                     /locus_tag="ACHJ1G_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4766177.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3945..4352
                     /locus_tag="ACHJ1G_004441"
     CDS             3945..4352
                     /locus_tag="ACHJ1G_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766178.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4545..4700
                     /gene="ydfA"
                     /locus_tag="ACHJ1G_004442"
     CDS             4545..4700
                     /gene="ydfA"
                     /locus_tag="ACHJ1G_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4766179.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4702..4803
                     /gene="ydfB"
                     /locus_tag="ACHJ1G_004443"
                     /pseudo
     CDS             4702..4803
                     /gene="ydfB"
                     /locus_tag="ACHJ1G_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            5764..5952
                     /gene="dicB"
                     /locus_tag="ACHJ1G_004444"
                     /gene_synonym="ftsT"
     CDS             5764..5952
                     /gene="dicB"
                     /locus_tag="ACHJ1G_004444"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4766180.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5949..6140
                     /gene="ydfD"
                     /locus_tag="ACHJ1G_004445"
     CDS             5949..6140
                     /gene="ydfD"
                     /locus_tag="ACHJ1G_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO4766181.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            6234..>8125
                     /locus_tag="ACHJ1G_004446"
     CDS             6234..>8125
                     /locus_tag="ACHJ1G_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4766182.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
BASE COUNT         2250 a         1991 c         1814 g         2070 t
ORIGIN      
        1 ctgatagtta acattacaag atataagtaa tggacgcact cccaattagt ctatttaaat
       61 cgccacgagt ttaactgaca acccatgatc aattatgaat tgcaactatt tctgtagtca
      121 cttttgtggg gacagtccac aaaactgcca acttccgctt cttgctctta gcggacatta
      181 gcataggcta tttaccataa cgcctcatta cgcgcaccgc ccagactgac tcagcgcgtt
      241 tctggcatat ccccggtaaa acaagtaaca aaccacccga aaatgaacat cagaaacgcg
      301 acttaagaat ctaccctatg aatggatatg cactcaaccg aatcgatctt ggtttcaatc
      361 tattttatcg ggatcaggct tctttttagg taacttcggg ggcttaactt gctgatgact
      421 ttgcgttcgg cgcgtaagcc agggatggtc agctttagat ttaacatagt attttgagcg
      481 taaatcaata cgggcattat ccactcgttc atggacactc ttttcatcat ccagtggtat
      541 aggctccggg ccatcaacat acttttccca gcccagcgct ttcccgtcat acagaacgtt
      601 aatttcaccg tcaaagttct cacatacagt aacaaccgaa tgcctaagtc gatatccccg
      661 gccctcactg cgtacctgaa acgcactgct tttgtactgg aaagtgagat ttttagacag
      721 aacgcgcttc gcctgtaggc tgaagatata acccagttcc tcttcagaat ggtgcacatc
      781 aagatgagca ttatcagtag tacgaggcga cgtagcgaac cggttgttat aggcttcaat
      841 aaaggtcggc aaccatgcat ttgctgtttc aatatcactg atattctgaa gccgcatttc
      901 tttgaccagc ctgtcctgta gtgtctgatt ggcgcgctct acccgccctt ttgcctgcgg
      961 gctgttggca tggattggct cgatgcccag tgtctttatc gcacgagtga actgggtcag
     1021 ctcaccttcc cgctctgggt tatttaccct gaatatactg tgtctatcag agtagagagc
     1081 gagcggtacg ccatgatcat taaggtaacc ccggagggtt tccatgtaag cccgggttgt
     1141 ttcagcaggc acaaaacgca acgccatcaa cgcactggtg gcatcatcaa tgaaaacgat
     1201 cagtgtacat ctggggcctc gattttcaaa ccagtcatga ggtgagccat caatctggat
     1261 cagttcaccg taagatggtc gtcgcatgcg gcgttgatat atacgggcaa ttttacgacg
     1321 gcgttcacgc cataacccct cttctatcat ccactttctg agagtttcaa cggataaaga
     1381 taaaccgtgt atctcgcgca atttttcgca cgcaaacgta ggtccaaaat cagcgtaacg
     1441 gcctttgagg agtgaaatta ctgttgctct gaattcagta gaaaaggaat tattaggacg
     1501 ctttccacgt cggtgggaaa ccagaccaga aggcccttca tttctatacc gttgcaccag
     1561 acgtttaacc tgccgaatag aaatgccaat gcgtgccgca gcttgttcct gagtaatatg
     1621 tcgattaatt gactcctgaa taatctgaag tcggtggagt tccttatgac tcatcgtaac
     1681 agtctctttg atcatgaaaa atcccccaga gaattgtctg gggacatttt agaatggttc
     1741 aaaggggaca ttacagcttg gtgttaacaa tagcctgctt ctccttgcct tccggcactc
     1801 gcgacagatt atgtttagac gactgtcgta gcgacgtatt tctccgtcag gtaatgacca
     1861 gataaggtcc ggatcaacca cagcaggttt cttcaccttt gccctcgaga gttttttgcg
     1921 ggcgttttgc cagtccttac gcgcctgttc agacgggaat aacccgtaac cagagctgta
     1981 tacatcgcca ctggcaacca gctctctggc cagaacgctc atcagatatc ttgttgcccc
     2041 agttttagct tccagttgtc gtaacgtctc gcgcccactc tggcgtacga gttccaccac
     2101 ctgcccttta attttttccc gctcttcttg tgtaaaaact tttgccacaa gtcctcctga
     2161 acattacttt ataccctaaa atcaacactt atcccctgaa accaggcgga atttctgtat
     2221 ccggttcaga aatatgattc acacaacgct gtacaggtga acgtcccagg cggatgacca
     2281 gttcgtccca tttttcgcgg agttttgacg ggctcatgat gttttttacc cagaattgat
     2341 ctcgctgtac ccgaccaaac atttcgcaaa tttgtctgtg gcttctgcca tccagcatcc
     2401 gcattgtgcg cacgtcattg gcccaggctg tccagttagg ctcttttggt cgcatgatct
     2461 ctccatcgtc gctggcagcc tgttcgtaaa ggttcacgat ccgcccccaa atccactgcg
     2521 cacacgccaa atcctccctg ctaccccact ggcgtttttt cgcactaaac acaaccgcgt
     2581 cggggttccg ggttaaaaaa tcctgttcag cggtctgctg gtccggttgc gaagcttccg
     2641 gacgaaaagt gtttttattc tctgtagtaa tctctgttgt attctctgta agatcatcag
     2701 gccattttga cccgatgaca ttgggtcgtt ttgaaccaat ggagcgtttc attttgacct
     2761 cttccatcgt gtcattttga cctgatggag cggcgcattt taaaccgatg gattcgctca
     2821 atttgccacc atctaaaagc tcgctcccgt agttgatcgt gtagaaattg gtcatatcgc
     2881 gctttgattt attgagcttt tcacaacgca aaagccccag cgttttcaga cttgcaaacg
     2941 cgcgctttaa cgttgactct gaccagaatg ggaactgttc cagccattgt tccgttgtgt
     3001 tgtaaatcca gcgaacacca tcacattcca tgccggaatt ggtatctctc aaccagtaat
     3061 gcaactgctg caacacaatg gcttcattta agccaatctt catcgcaagc tgtgtgttta
     3121 taaccagtgg gcgttcagca aaaagaaggc tcataattcc atccagcttt ttgttggtat
     3181 tgctgtcgat acgcaagttt gaaagcaatt gctttttcta taagttcgtc agtttcacga
     3241 tccactacgg caggatcagc aaaaagcagt ccggactcca ccacatcgcc atattctttg
     3301 tttaacccgg cgatcatgta cgtgatgctt tttccgtcac taatttcacg atacaacctg
     3361 aaatcattaa tccgaatagc ctccataatt gcaggcacta gcgccgtgaa cttttcacgc
     3421 ttatccctgg tgtcgatagc cttccagcgt tcgaatatct tcactcgatt aacgccaagt
     3481 gctcgctgat caaccgcgcc accttcatct gtgacacgct gaacatcgat gttcgggcgc
     3541 tctttcaaag cccagaatgc ttcagtgatt aatatcgtcg cctgctcctg tgtcattcct
     3601 ggtcgacata tccaggcatc cagagcctca cgagcctgtt caggagtgat tttcattgtt
     3661 caaccgcccc gcccgcttcg tcttacgata ttcgtcataa accttgggat catacagaag
     3721 ctcgccgcca gatgcctcct gtagacgcat cgcgcgacct tcaggaacca gctcttgcca
     3781 ttgagaaaca gcagatgggt caacgccagc agctttcgct actttggctt tcgtaccgta
     3841 aaaattaatt acgtctgatt taaacatcgc acctccaata ttgagttttc tcaatgctaa
     3901 tcactcaagg aatctcaagt caagggttat taagatatct aaatatgaac gagaaaactt
     3961 taggtcaacg cattagagaa agacgtaagc aggtaggctt aagtcaaaac ggtttaagca
     4021 aagctgctgg cgtatctggc tcatcaattt cattatggga aagtgaccat acagccccgc
     4081 gcgggcaaaa tttgcatcgc ctcgccgagg tattgcaatg ttcaccaact tggatactgt
     4141 ttggcgacga ggacaaaaca ccagctcccc ctgtttcact cgatagtgcc ttagacttat
     4201 cggaagatga gttagagatg ctgcgtttgt atcgcgcact tcctaaatca gagcaacaag
     4261 cacaactcag cgaactccgc gcccgcgttg agaattttaa tcgcctgttc accgagctat
     4321 tagaggctcg caaacgcaac aagcatcaat aatcatccct tcacaaaatt ttaaagcctt
     4381 acatttcaat gtattggctt tatcttgcgc caacacttga gttttctcat caaaaaacct
     4441 tgacgaaaaa taatgagaaa actaaattac ctccatcaag acaccgcacg gtgttctcag
     4501 caaacagttc cgctaccccg gcgttaaggg gaaatgaggt cagcatggat actatcgatc
     4561 ttggcaacag cgaatctctg gtatgtggcg tgttccccaa ccaggacggc acgttcaccg
     4621 cgatgacgta taccaaaagc aaaacgttta aaaccgaaaa tggtgcccgt cgctggctgg
     4681 aaagaaactc aggtgagtga tatggatttc aacacaatca tggaaaaggc ttacgaagaa
     4741 tacttcgaag gccttgccga aggcgaagaa gctctcagct tcagtgagtt taaacaggca
     4801 ctcagaataa gaatgtgctc tcacaatgac gcggagcaca aatatgagaa gcaaaatcag
     4861 accgcagaaa attttgttct ggaacccgga gaaacgcttt tcaaaattcc cgttacgtgc
     4921 cccatttgcg gttttacatc agaagaactt gacgactcct gtaacaatca ggaaacaacc
     4981 aagtatgtcg aagatgatac cgagtgcgca cgaagaacga ttatatccac gagtccaaac
     5041 tccaggacca ataaatctca ctatgagagg gtgattaatc cactccccca aaccaataaa
     5101 aaagatgccg gaggacaaaa aacccaatgg aacaacggga tatctgtcaa aaaaagacgt
     5161 tccattaaag acaaacaacg cagcgccaac aactgtaagc gctttatacc agtaatcaat
     5221 tttcatgttc ttaagcggat ttattggtgg ttgcgacatt gcttaatgaa tccttaaaac
     5281 tgtggtgatt ttaaggatac cacctcgcct gacgtggtta aaagcaggca cacaacacga
     5341 aagcgcacgg cgaagttcgt ctcactgtac ggtgtcgtta aatttaattc gaccgtgcgc
     5401 ttccggttgt ggcaacccgc aaaatggcgc ggcggtaagt atggcggggt tattccttcc
     5461 ccgttgagga caccgggttg tcaggttgac catacgctta agtgacaacc ccgctgcaac
     5521 gccctctgtt atcaattttc tggtgacgtt tggcggtatc agttttactc cgtgactgct
     5581 ctgccgccct ttttaaagtg aattttgtga tgcggtgaat acggctaagc gcacgcggaa
     5641 cagttaaaac caaaaacagt gttatgggtg gattctctgt atccgacgtt aattgttaac
     5701 tggttaacgt cacctggagg caccaggcac tgcatcacaa aattcattgt tgaggacgcg
     5761 ataatgaaaa cgttattacc aaacgttaat acgtctgaag gttgttttga aattggtgtc
     5821 actatcagta accctgtatt tactgaagat gccattaaca agagaaaaca agaacgggag
     5881 ctattaaata aaatatgcat tgtttcaatg ctggctcgtt tacgtctgat gccaaaagga
     5941 tgtgcacaat gaattcagca tttgcgcttg ttctgacagt ttttcttgtt tccggagtgc
     6001 cagttgatat tgcagtcagt gttcacagga caatgcagga gtgtatgact gcagcaaccg
     6061 aacagaaaat tcccggtaac tgttacccgg tcgataaagt tattcaccag gataatatcg
     6121 aaatcccggc aggtctttaa aacagttccg taataaatat ccggtttcat tcttatatgc
     6181 cagcaatggc agggatttgt tcatccttaa atctgtcatg aggttaaaac aaaatgagta
     6241 aagtctttat ttgcgccgct attcctgacg aactggcaac aagggaagaa ggctctgtgg
     6301 ctgtagccac agccattgaa gctggcgacg aacgccgtgc tcgagcaaaa tttcactggc
     6361 agttcctgga acattatccg gctgctcagg actgcgctta taaatttatt gtctgcgagg
     6421 ataaacctgg cataccccgc cctgccctcg attcatggga tgctgaatat atgcaggaaa
     6481 accgctggga tgaggagtct gcttcttttg tcccggttga gactgaatcc gatccgatga
     6541 acgtcacttt tgacaagctg gcccctgaag tacagaacgc tgtcatggtt aagttcgaca
     6601 catgtgaaaa catcaccgtt gatatggtta ttagcgcaca ggaattgttg caggaagaca
     6661 tggcaacatt cgacggacat atcgttgaag cgttgatgaa aatgccagaa gttaacgcca
     6721 tgtatccgga gcttaagttg cacgccattg ggtgggttaa gcataaatgt attcctggtg
     6781 ctaaatggcc cgaaattcag gcagagatgc gcatctggaa aaaacgtcgc gaaggtgaac
     6841 gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc ccgcgccaga gccaatcaac
     6901 agtacactga aaattcaaca ggaaaaatca gcccggtcat tgctgccatt catcgcgaat
     6961 acaagcagac atggaaaaca ctggatgacg aactggccta cgctctctgg cctggtgatg
     7021 tggatgccgg aaacattgac ggcagcatcc atcgctgggc aaaaaaagaa gttatcgaca
     7081 acgaccgcga agactggaag cgtatctcgg catcaatgcg caaacagcct gatgcccttc
     7141 gctacgaccg ccaaactatt tttggccttg tccgtgagcg tccgatcgac attcacaaag
     7201 atcccgtagc actgaacaaa tatatctgcg aatacctgac gacaaagggc gtgtttgaga
     7261 atgaagaaac agacctgggc actgttgatg ttctccagtc atcagaaaca caaactgatg
     7321 cagtggaaac tgaggtatct gatatcccaa aaaatgaaac cgcgccggaa gctgaaccat
     7381 ctgtagagcg tgaggggccg ttctatttcc tcttcgcaga taaggacgga gaaaaatacg
     7441 gtcgcgcaaa caaactttct ggtctggata aggcactggc tgctggcgcc actgaaatca
     7501 caaaagaaga atattttgcc cgaaaaaatg gcacatacac gggcttaccg caaaatgtag
     7561 ataccgctga agattcagaa caaccagagc cgataaaagt taccgctgac gaagtaaaca
     7621 aaattatgca ggcagccaat atcagccagc ctgacgccga taagttgctt gctgcatcac
     7681 gtggtgaatt tgttgaaggg attagtgacc cgaatgatcc gaaatgggtt aaggggatcc
     7741 agacccgcga ttctgtgaac cagaaccagc atgaatcgga acggaactac caaaaagcgg
     7801 aacaaaacag tccaaatgcg ttacaaaacg agccagaaac gaaacagcct gaaccagtgg
     7861 cgcaacagga agtggaaaaa gcctgcaccg cctgcggtca gaccggcggc ggcaactgcc
     7921 ctgattgtgg cgcggtgatg ggcgacgcaa cataccaaga aacattcgat gaagagtatc
     7981 aggttgaagt tcaggaagat gatccggagg aaatggaagg cgctgaacat ccacacaagg
     8041 agaacactgg cggcaatcag catcacaata gcgataatga aactggcgag acggcagatc
     8101 acccaattaa ggtgaacggt catca
//
LOCUS       DAZFDP010000187         8112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFDP010000187 DAZFDP010000000
VERSION     DAZFDP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..881
                     /locus_tag="ACHJ1G_004447"
     CDS             51..881
                     /locus_tag="ACHJ1G_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4766183.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            913..1848
                     /locus_tag="ACHJ1G_004448"
     CDS             913..1848
                     /locus_tag="ACHJ1G_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4766184.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1941..2183
                     /gene="yhdT"
                     /locus_tag="ACHJ1G_004449"
     CDS             1941..2183
                     /gene="yhdT"
                     /locus_tag="ACHJ1G_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4766185.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2173..3624
                     /gene="panF"
                     /locus_tag="ACHJ1G_004450"
     CDS             2173..3624
                     /gene="panF"
                     /locus_tag="ACHJ1G_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4766186.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3636..4517
                     /gene="prmA"
                     /locus_tag="ACHJ1G_004451"
     CDS             3636..4517
                     /gene="prmA"
                     /locus_tag="ACHJ1G_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4766187.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4846..5811
                     /gene="dusB"
                     /locus_tag="ACHJ1G_004452"
     CDS             4846..5811
                     /gene="dusB"
                     /locus_tag="ACHJ1G_004452"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4766188.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5837..6133
                     /gene="fis"
                     /locus_tag="ACHJ1G_004453"
     CDS             5837..6133
                     /gene="fis"
                     /locus_tag="ACHJ1G_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4766189.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6219..7103
                     /gene="yhdJ"
                     /locus_tag="ACHJ1G_004454"
     CDS             6219..7103
                     /gene="yhdJ"
                     /locus_tag="ACHJ1G_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4766190.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7187..7366
                     /gene="yhdU"
                     /locus_tag="ACHJ1G_004455"
     CDS             7187..7366
                     /gene="yhdU"
                     /locus_tag="ACHJ1G_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4766191.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7369..8031)
                     /gene="acrS"
                     /locus_tag="ACHJ1G_004456"
     CDS             complement(7369..8031)
                     /gene="acrS"
                     /locus_tag="ACHJ1G_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4766192.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2003 a         1965 c         2044 g         2100 t
ORIGIN      
        1 aactttcaaa gaaaaataca aggggctata gtgttacgca ttaaaggcca atgaagagtg
       61 ttaacgtgcc gagaaaatcg tctaacctac tgaaacgcag attaaaaatt gcagaaattg
      121 ttgccgccca aggtgaaatc aaagttgatg acttatctgc gcaacttggt gtctctggcg
      181 tgacgatccg cggcgatctc agttatctgg agcaacaggg atatttaaag cgatcatttg
      241 gcggtgctat tgcgacacca ctctcctctg aaccagaagc gatacaaccg atcgcgccat
      301 tgtctctggc acaacaaatg gaaatagcac gacattgcgc acggttaatt cgcgatcgtg
      361 acacgctatt tcttggacat gggacaatct gccgtaagat cattccattg ctcagcgaag
      421 tgaaaaaact gcgtctgatc accaacgatc ccgaacatgc gctgctggca aatcagttta
      481 ttgatggcga aataatactg gctggcagtg aaatgctgcg ccccgacacc gcactggccg
      541 gaaaaccgct tgaacaggca cttcagcatt tcaccattac acatagcatt ctggaaatca
      601 gccatctgga tgctgatggt actttaaata tcaacgttcc cagactggcc gctgcctggc
      661 agctctgttt cgatcatgcc cagaataaga cggttatcgt tgccgccgat cctgcgccat
      721 caacggctgc tatcgggcat ttgcatcagg cagaaaatgt gatcgtcagc cgcagcatta
      781 acgaacaata tcagcaacaa ttgcagtcgg ctgatttcgt tatcctttat accagtaatg
      841 agtgctttac ctggtccaat cgcgaaaggc ttcaggaatg agaggggttt tgtgacaata
      901 actatctgcg ctatgggcga attgctggcc gagtttttgt cccgcaaccc acatcaaaaa
      961 ttcactcagc ctggggagtt tatcgggcca tttcccagcg gtgcgccagc aatttttgct
     1021 gctcaggtgg caaaactgtc ccatcgggcc atcttcttcg gatgtgttgg taatgatgat
     1081 tttggccgac tcattataga gcgcctccgt catgaaggtg tcattaccga tgggatccat
     1141 gttatgaaca atgccgtcac aggtacggcg ttcgtgagtt atcaaaatcc ccagcagcgc
     1201 gatttcgtct ttaatatccc taacagcgcc tgcggcttgt ttactgctga gcacattgat
     1261 aaggatctgc taaaacagtg taaccatctc catattgtgg gctcatcgtt gttctcattt
     1321 cgcatgatcg atgtcatgcg taaagcaata acgacgatca aatcggctgg cggcaccgtt
     1381 tctttcgatc ccaatattcg caaagagatg ctgagcattc ctgaaatggc gcaggctctc
     1441 gattatttga ttgaatatac ggatattttc atccccagcg aaagcgaact ccctttcttc
     1501 gcgcgtcaca aaaatctgtc agaagaacag attgttagcg atcttctcca cggcggcgta
     1561 aaacatgtgg cgataaaacg cgcccagcgt ggggccagct attacaagct taaaaacggt
     1621 acattacacg cccagcatgt tacaggccac gatatcgaaa ttatcgatcc aacgggtgca
     1681 ggcgactgct ttggcgcaac gtttatcact cttttcttat ccggtttccc ggcacataag
     1741 gcactgcaat atgcaaatgc cagcggcgcg ctcgccgtaa tgcggcaagg tccgatggaa
     1801 gggatatcct cactggctga cattgaagac tttttgcagc agcactgaac ggcaattttc
     1861 tgtcagatta ttttccctgc ctgaagagtt gccgtagaat tgcccgtttc tcgtatcccc
     1921 ccatgaggag gaacccttcc gtggacactc gttttgttca ggcccataaa gaggcgcgct
     1981 gggcgctggg gctgaccctt ttgtatctgg cagtttggtt agtagccgct tacttacctg
     2041 gcgttgcccc cggttttacc ggctttccgc gctggtttga gatggcctgc atcctgacgc
     2101 cgctgctgtt tattggactg tgctgggcga tggtgaaatt tatctatcgc gatatcccac
     2161 tggaggatga cgatgcagct tgaagtaatt ctaccgctgg tcgcctatct ggtggtggtg
     2221 ttcggtatct cggtttatgc gatgcgtaaa cggagcaccg gcaccttcct taatgagtat
     2281 ttcctcggca gccgctctat gggcggtatt gtgctggcga tgacgctcac cgcgacctat
     2341 atcagtgcca gttcgtttat cggcgggcca ggagctgctt ataaatacgg gctgggctgg
     2401 gtattgctgg cgatgattca gcttcctgca gtctggcttt cactcggtat tctcggcaag
     2461 aagtttgcga ttcttgcgcg ccgctacaat gcagtgacgc tgaacgatat gctgtttgcc
     2521 cgctaccaga gtcgtcttct ggtgtggctg gcgagtttga gtttgctggt tgcgttcgtt
     2581 ggtgcgatga ccgtgcagtt tatcggcggt gcgcgcctgc tggaaaccgc ggcgggtatt
     2641 ccttatgaaa ccgggctgct gatttttggt atcagcattg cgttatatac cgcctttggt
     2701 ggctttcgcg ccagcgtgct gaacgacacc atgcaagggc ttgtgatgct gattggcacc
     2761 gttgtgctgc ttattggcgt ggtacatgcc gctggcggct taagcaacgc agtacagacc
     2821 ttgcaaacca tcgatccgca actggttacg ccacaaggcg ctgacgatat tctgtcgcct
     2881 gcctttatga cgtcgttctg ggtactggtg tgctttggcg tgattggcct gccgcatact
     2941 gcggtgcgct gtatctctta taaagacagc aaagcagtac accgggggat catcatcggt
     3001 acgattgtag tcgcaatcct gatgttcggt atgcacctgg ccggagcgtt aggtcgtgca
     3061 gtgatccccg atctcaccgt accggatctg gtgatcccga cgttaatggt aaaagtgctg
     3121 ccaccgtttg ctgccggaat cttcctggct gcacctatgg ctgcgatcat gtcgacgatc
     3181 aatgcccagt tgctgcaaag ttccgctacg atcattaaag atctctatct gaatatccgt
     3241 ccggatcaaa tgcaaaacga gacgcgtctg aagcggatgt cggcggtaat tacgttagtt
     3301 ctcggcgcgt tgctgctgct tgccgcctgg aagccgccag aaatgatcat ctggctgaat
     3361 ttgctggcct tcggtgggct ggaagccgtt ttcctgtggc cgctggtgct gggtctttac
     3421 tgggaacgcg ccaacgccaa aggcgcgcta agtgcgatga tcgttggcgg cgtgctgtat
     3481 gccgtactcg cgacgctgaa tattcagtac ctgggcttcc accctattgt gccctcgtta
     3541 ctactaagtt tgctggcttt cctggtcgga aaccgtttcg gtacatccgt cccgcaagct
     3601 accgttttga ctactgataa ataaagagtt ttgccatgcc ttggatccaa ctgaaactga
     3661 acaccaccgg cgcgaacgcg gaagatctta gcgatgcgct gatggaagcg ggtgccgttt
     3721 ctatcacttt tcaggatacc cacgatacgc cagtatttga gccgctgccg ggcgaaacgc
     3781 gcctgtgggg cgacaccgat gtgattggtc tgttcgacgc tgaaaccgat atgaacgacg
     3841 tggtggcgat tctggaaaac catccgctgc tcggcgcagg cttcgcgcat aaaatcgaac
     3901 aactagaaga taaagactgg gagcgcgaat ggatggataa tttccacccg atgcgctttg
     3961 gtgaacgact gtggatctgc cctagctggc gtgatgtgcc ggacgaaaac gccgtcaacg
     4021 tgatgttaga tccagggctg gcgtttggta cgggtaccca tccaaccacc tctctgtgcc
     4081 tgcaatggct cgacagcctc gatttaaccg gtaaaacagt catcgacttt ggctgtggtt
     4141 ccggcattct ggcgatcgcg gcgctgaaac tgggtgcagc aaaagccatt ggtattgata
     4201 tcgatccgca ggcgattcag gccagccgcg acaacgccga acgtaatggc gtttctgacc
     4261 gtctggaact ctacttaccg aaagatcagc cagaagaaat gaaagccgac gtggtggtcg
     4321 ctaacatcct tgcaggccca ttacgtgaac tggcaccgtt aatcagcgtc ctgccggttt
     4381 caggcggttt gctgggcctt tccggtattc tggcaagcca ggcagagagc gtttgtgaag
     4441 cttatgccga tagcttcgca ctggacccgg tcgtggaaaa agaagagtgg tgccgtatta
     4501 ccggtcgtaa gaattaacct tcgcatcgcc gtagggtgac gcggggcgaa gtgcgagcaa
     4561 gctcacaaaa ggcacgtaaa tttgccggtt atttacgcaa atttgcgtgc caaaattttc
     4621 attcataaag aaaaattgag aacttactca aatttctttg agtgtaaatt ttagtcacta
     4681 ttttctaata tgatgatttt tatgagtaat tatcgcacca cgctcatttt aaatgcaatt
     4741 ctttgatcca tctcagagga ttggtcaaag tttggccttt catctcgtgc aaaaaatgcg
     4801 taatatacgc cgccttgcag tcacagtatg gtcatttctt aactcatgcg catcggacaa
     4861 tatcagctca gaaatcgcct gatcgcagcg cccatggctg gcattacaga cagacctttt
     4921 cggacgttgt gctacgagat gggagccgga ttgacagtat ccgagatgat gtcttctaac
     4981 ccacaggttt gggaaagcga caaatctcgt ttacggatgg tgcacattga tgaacccggt
     5041 attcgcaccg tgcaaattgc tggtagcgat ccgaaagaaa tggcagatgc agcacgtatt
     5101 aacgtggaaa gcggtgccca gattattgat atcaatatgg gttgcccggc taaaaaagtg
     5161 aatcgcaagc tcgcaggttc agccctcttg cagtacccgg atgtcgttaa atcgatcctt
     5221 accgaggtcg tcaatgcagt ggacgttcct gttaccctga agattcgcac cggctgggca
     5281 ccggaacacc gtaactgcga agagattgcc caactggctg aagactgtgg cattcaggct
     5341 ctgaccattc atggccgtac acgcgcctgt ttgttcaatg gagaagctga gtacgacagt
     5401 attcgggcag ttaagcagaa agtttccatt ccggttatcg cgaatggcga cattactgac
     5461 ccgcttaaag ccagagctgt gctcgactat acaggggcgg atgccctgat gataggccgc
     5521 gcagctcagg gaagaccctg gatctttcgg gaaatccagc attatctgga cactggggag
     5581 ttgctgcccc cgctgccttt ggcagaggtt aagcgcttgc tttgcgcgca cgttcgggaa
     5641 ctgcatgact tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca cgtttcctgg
     5701 tatctccagg aacacgctcc aaatgaccag tttcggcgca cattcaacgc cattgaggat
     5761 gccagcgaac agctggaggc gttggaggca tacttcgaaa attttgcgta aacagaaata
     5821 aagagctgac agaactatgt tcgaacaacg cgtaaattct gacgtactga ccgtttctac
     5881 cgttaactct caggatcagg taacccaaaa acccctgcgt gactcggtta aacaggcact
     5941 gaagaactat tttgctcaac tgaatggtca ggatgtgaat gacctctatg agctggtact
     6001 ggctgaagta gaacagcccc tgttggacat ggtgatgcaa tacacccgtg gtaaccagac
     6061 ccgtgctgcg ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat tgaaaaaata
     6121 cggcatgaac taattcaggt tagctaaatg cttgattaaa aaggcgctac tcggcatggg
     6181 gaagcgcctt ttttataggt gtcacaaagg gagtgaccat gagaacagga tgtgaaccga
     6241 cccggtttgg taatgaagct aagaccatta ttcacggtga tgcgcttgcc gaacttaaaa
     6301 agatccccgc cgaaagtgtc gatctgatct ttgccgaccc accatataac atcggtaaaa
     6361 attttgatgg tctgatcgaa gcctggaaag aagatctgtt tatcgactgg ctgtttgaag
     6421 tgattgcaga gtgccaccgc gttctgaaaa agcagggcag catgtacatc atgaacagca
     6481 cggaaaacat gccctttatc gatctccagt gccgcaagct ttttaccatc aaaagtcgca
     6541 tcgtctggtc atatgacagt tctggagtac aggcgaaaaa acactacggc tccatgtacg
     6601 aacccatcct gatgatggtg aaagacgcaa agaactacac attcaacggt gatgctattc
     6661 tggtagaagc caaaaccgga tcgcagcgcg cgttgatcga ttatcgcaaa aatcctccac
     6721 agccatacaa tcatcaaaaa gtaccgggta acgtctggga ttttccgcgc gtgcgttatt
     6781 tgatggatga atatgagaac cacccgacgc aaaaaccgga agccttactg aaacgcatta
     6841 ttctcgcctc ttccaaccca ggcgatatcg ttctcgaccc gtttgccggt agctttacta
     6901 ccggtgccgt agccgtctcc agcgggcgaa aattcattgg tattgagatc aacagcgagt
     6961 acatcaaaat ggggcttcga cggctggatg tcgcgtcgca ttactctgcg gaagaactgg
     7021 cgaaagtgaa aaaaagaaag accggcaacc tgtcaaaacg aagccggtta agcgaagttg
     7081 accccgatct cattgcaaag taaaggaagt gtaagcctgg ttttcagatt attcatttcg
     7141 tgtatatttc ctgccagact tggttaaaca tacacaggct caggtaatga ttcgcaagta
     7201 ttggtggctc gtcgttttcg ctgtcttcgt ttttctgttt gatactttac tgatgcagtg
     7261 gattgaactg ctggcaacag aaacagacaa atgccgcaat atgaactcag ttaatccact
     7321 aaaactggtt aactgtgacg aactgaattt tcaggacaga atgtgaattt acatgacact
     7381 taattcattc gtttgatgaa ttaatttcgt tatgttttca tctggcatga acattcttaa
     7441 tacgttatcg accagagcgg gggcttgttt ataaagatca taacccgcca tattcattaa
     7501 ccagttttga acaattccgc tgaaggcacc attaataata atcatcacaa catctaaatc
     7561 gaggttattt gctacacaac cttgttgctg acacgcctgc aatacttcgc ggagagtctg
     7621 cggattgaag cccatctttt cgcgtatcac tccctcggcc agcatctcat cattaaattc
     7681 acatttgtga tataagattt tcagcaacgc ctgctggcgg ggaattttgg caatatattg
     7741 caagccgaca atcaattttt cacgcaattg ttgaaacggg tcatgctcta atccagccgt
     7801 caagtgttcc tggattaact cccgcaatga aggctgttgc aaccacatct cattaaacag
     7861 ttgagtcttg ttttcgaagt gccagtagat agcgccacgc gtaacgttag cagcgtcggc
     7921 aatgtcgttg agtgtcgtct tgcttacgcc atgctgcgca aactgggcga tggcagtttc
     7981 aatcagttct tgccgggtct tcagagcttc ggctttggtt ctttttgcca tgattaatta
     8041 ttcaggaaat aaatatattc gacacagagt gagaaaatag cgaaggttaa tctatcacct
     8101 aatgtgtatt ta
//
LOCUS       DAZFDP010000188         8108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFDP010000188 DAZFDP010000000
VERSION     DAZFDP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..1013)
                     /gene="pyrD"
                     /locus_tag="ACHJ1G_004457"
     CDS             complement(3..1013)
                     /gene="pyrD"
                     /locus_tag="ACHJ1G_004457"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4766193.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1124..1861)
                     /locus_tag="ACHJ1G_004458"
     CDS             complement(1124..1861)
                     /locus_tag="ACHJ1G_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4766194.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1827..2342)
                     /locus_tag="ACHJ1G_004459"
     CDS             complement(1827..2342)
                     /locus_tag="ACHJ1G_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766195.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2350..2892)
                     /locus_tag="ACHJ1G_004460"
     CDS             complement(2350..2892)
                     /locus_tag="ACHJ1G_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766196.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2904..3974)
                     /locus_tag="ACHJ1G_004461"
     CDS             complement(2904..3974)
                     /locus_tag="ACHJ1G_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766197.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3965..6565)
                     /locus_tag="ACHJ1G_004462"
     CDS             complement(3965..6565)
                     /locus_tag="ACHJ1G_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4766198.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6590..7291)
                     /locus_tag="ACHJ1G_004463"
     CDS             complement(6590..7291)
                     /locus_tag="ACHJ1G_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4766199.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7374..7916)
                     /locus_tag="ACHJ1G_004464"
     CDS             complement(7374..7916)
                     /locus_tag="ACHJ1G_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766200.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         2103 a         2041 c         1771 g         2193 t
ORIGIN      
        1 aattagatat gggtaacgat ttctttaatc agcggcggac ctttaaaaat aaaaccagaa
       61 taaatttgca ccagtgaggc acccgcagca atcttttcac gcgcagcgat aaccgagtcg
      121 atgccgccaa caccgatgat cggtaagcga ccgtttaatt ccagtgacaa gcggcgaata
      181 atttcggtgc tttttaactg aagcggacga ccacttaagc caccggtttg atcgcaattt
      241 ttcattccct gaacaagaga acgatcgagt gtggtattgg ttgcaataac gccatcaata
      301 ttatggcgaa ctaaactatc ggcaacctgg atcaattctt cttcagaaag atccggcgcg
      361 atcttcactg cgatcggcac atatttatgg tgcatcgctt gcaaatcatt ttgcttattt
      421 ttaatcgcgg ttaagagatc atccagcgct tcaccatatt gcagcgtgcg taatcctggg
      481 gtattcggcg atgaaatatt gatggcgata tatcccgcat aggcatagat tttttccata
      541 caaatcagat agtcatcttt gccctgctcc actggcgtat ctttattttt gccgatgtta
      601 atacccagga cgccgtcata atgggccttt tttacgttct ctacgaggtt atcaacgcca
      661 agattattaa agcccatacg gttgatcaaa ccttcggcat ctaccagacg aaagagacgc
      721 ggcttgtcat tacctggctg tggacgtggc gtgacggtac cgatctcgat cgatccaaat
      781 cccatcgcgc ctaacgcgtc aatgcactcc ccgtctttat caagaccggc tgccagacca
      841 agcggatttt taaacgtcag gcccatgcag ttaacaggtt tcgcaggcac tttctgccgc
      901 accagtgctt caaacggcgt tcctgtaata cggcgtaatt gctgaaaagt aaactcatga
      961 gcgcgctctg gatcgagctg gaaaagggct ttacgaacga aggggtagta catgaactct
     1021 cctggattcc cggtgtgcaa accgggggcg tattatgtgc gagttcggac caaaagggaa
     1081 ttgacctgcg gcaaaaaaaa cgcaaacgtt ttccgaacct gtttcagggc agattttgct
     1141 ccttctttgc actcaacccg ccgtaatcat ttatcgcccg ccaggcgaca tgacacggtg
     1201 cgattgcctg acaccagctt gtctgacgct ggcttttagg cggcacaact cccgcattac
     1261 ttaacgcttt accatttacg ctcaactgaa tcaggttaat gtagtaaggc gttgggttag
     1321 tgagttgtac accctgcgaa ttccgtgtcc agcgtaattg ttgataagct tccagcgggt
     1381 cgcccggcaa accttcgggt cgccagaaca gtttaaatac cgagcgcatc gccactttta
     1441 cgctctgatt ttcaactttg ccggatggca cgctggcaat gcttaactca aatagcgttt
     1501 cgcgatccac aggcaagatg tcgctttccg ttctcagcaa gcgcaacgtg cctgtagtac
     1561 cgggcttcag gagaataagt ggcggagcgg ccagtaatgg tgctggcact gtcgacgctt
     1621 cccccccggc ccaacgcgtt gggcggttga ttttactgtt aataagccag gattcctgac
     1681 tggtattagt cagtaaaata gatatcgatt ctctgtctgc cggaaagata aatcgcgttc
     1741 cgccgaccac cactcctgcc cgcaattcac ccgccaccag cagcgataaa acggcgaaaa
     1801 tcaagagcgc caatctgttc cacgtattag tcataatgaa gtacaaatgt cgcgctggca
     1861 ttcactttac ccgactgcac atcgctattg acaggtcgca attgcgcata aaaagttaat
     1921 ggcaccttac cgctatgtag gctgtagtct ttactcgcct ggttaagcgg aatcaatatc
     1981 ttcgcatcat ccagaatcgc aattgccagc ccgctggctg catccatacc actttcaatg
     2041 gacagcattt cgctctgcac tccgtcgggg gttcctgaaa aggtgacgtc aaccgtgctg
     2101 acatctgttc cgcaatcctg caaattaatg atgaaagatt tctccgggcc ataattcccg
     2161 tgagaaaaac gtgtcagcga gacatcgcct aactgcacat tgatttgctt gctatcctgt
     2221 gccagcacgc atgtattacg acgaaacgat ccgctaatgt tgaccgtgcc atcatgagca
     2281 aataactcac tactccccgt taatcccagt aagcacagaa tccagattat ccgtttcaac
     2341 atcactcctt cactggtaaa ccagatcaaa atagagaacc gccgtcgcat taccgcccgt
     2401 tgctcccgcc tttgtggact tataacgaag ttgatatttg agtgtgttat ccccggcttt
     2461 taagggcgta agaggctgga cctgattgag cgggatttct tgctgacttt gcgcatcaag
     2521 aatttgcacg gcaatccccg ttgccatacc accgcttccg cctgtgtcag ttagtttaag
     2581 cagtgtcggc gcaagggtgt cagcctcgcc gctaaaaagg acgtccgcgc cagtaatacc
     2641 agcggcacaa ccggtgatat cgatattcaa atcagctgca gtgcttacgg tattcgcggc
     2701 ctgaaaatca ctgatattaa aatcgccaat atgaatattt tgtaatgcac ttttggtggc
     2761 gacatcacag ccctgatcga ccacattccc ataaatatgc atactgactt gttcaggccc
     2821 agccatcccg acatggatgt taagaaggct aaagaacaaa ataacgtact gatatatcgt
     2881 ttttttcttc atacgttcac gcgttaatca aaatttattc tcaccgttgc cgtggcttca
     2941 aatcgcccgg tttctggtgg tacgcctgtc gtgctgactg gatagacatt cattcgcgcg
     3001 atactgtctg catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg
     3061 ttgttctggc tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg
     3121 ggatagctgg ggtctgaggt tgccgtcaga ctcaggttga cgttaactaa cccttcgttt
     3181 cccgtgttac acctgactgg aattgcgagt tctgccgttc tggccccaag gggtttatgc
     3241 cctacaacac gaaagttagc cgcaaatacg tcgcccagat cgacctcgat agtctctccg
     3301 gcattgacgg aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc
     3361 ggtgtacctg tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc
     3421 gccagcgcca taggaggaat aacgaccgaa ccaacgaacg gctgcaatat tttcagggaa
     3481 aagctaccgc tattaccgct agccccctgg ccacctaaac gttgaccgcc gggatcgcaa
     3541 atatgccatc gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa
     3601 ctgcctttaa cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg
     3661 tcgcctaaat agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg
     3721 tctttatcgc aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg
     3781 tctttaacta ctccagccgt attttcttct tcctccaggg ttttaacgcc aaaattgacg
     3841 ccaatatacg gattcccctg agtggtatga cactgcccgc tataagcagc agtttgagcg
     3901 caccatagca ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca
     3961 aagattatct gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa
     4021 gctggtaact gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc
     4081 cctgagcggg caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag
     4141 tctgtatgcc gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg
     4201 ttttcattaa tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt
     4261 tggtgggaac cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg
     4321 catcaagtgc gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc
     4381 gccagtcagt tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg
     4441 caatcagcac gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa
     4501 ctaaactgcc tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt
     4561 aacccactcg ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg
     4621 ttttcccctc gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta
     4681 gtgtctccgt taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag
     4741 aggtcatcca ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca
     4801 gattgagcgg cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg
     4861 acttactgta gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa
     4921 actgtaatag ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat
     4981 aaaacgaacc gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg
     5041 tgtaaaacaa attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa
     5101 attcgtagcc atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg
     5161 aatagcgata gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca
     5221 gaaaacgata tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg
     5281 tcgcatcgaa cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg
     5341 cacggtaata ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc
     5401 actgcaacgt ggcctgggca aatttgctct cctgctgttc attactattg gttctgtatt
     5461 tcgccagtgt caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa
     5521 acggtacgct gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac
     5581 cgctcgacga cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt
     5641 ggtaaatggt gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac
     5701 gcaccacagg cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct
     5761 gcacaccacg aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg
     5821 tcagttcgcc ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt
     5881 ctgcggtttg gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta
     5941 aattaacgcc actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc
     6001 tatgaatact gttagccccg ctaaacgaat atcccagtaa tagcgcgttg atcccttcat
     6061 cccaacgttc aggggggaca taaccacggg cattactttt gatggcgatt tgcggtactg
     6121 aaatttccag acggagttta ttcacattaa acgttacgct ggcatccgga ataatctccg
     6181 ccaatggcac acacgcttgt ttgtccagtt gtttaaaagc cggaaacgca tcagtattaa
     6241 cacccattgc atccaggctt tcagtcgtca aacaggcggc aatccccttc tctggcgtta
     6301 tctcaacaaa attgacgttg cgcgtatcta caattgtctg attcagaaca atatcgacac
     6361 gataaacccc tggcgttatt ttttgtcctt tttcgaaacg agacaaatcg gccaccattg
     6421 agggatcatc tttgaaaaaa gcaggatcga accaggtttc agcttgtgca ttgaacgctg
     6481 ccgacacaaa caccaccagc ccaccaataa acgatggcac tccaccagag cttaacaggc
     6541 tgtgttgtcg atgagttcta tacatctcgc tctcttaact gaagacggct tacgatttac
     6601 agttgagttg cggcgtttcc gccccgtagt cattgatggt tcgatacgtt acgctgctat
     6661 ttttggcccc ctttggcaga gaaaattggt atttatcaaa gggggaaatc atttgatttt
     6721 taactacttt tccacctatt ttcaggtctg tcagggtaat gaaataaggt gttgggttac
     6781 tgaccgtaac catgtcagct gacgaagtgc aacgcagttt cgccggagcc tcgcctaagg
     6841 gatacgctaa accttttggt cgccagaaaa gtttgatgac gctttgtgtg gcaatcttca
     6901 gggtatttcc ctcttcccgc ttcgttgttg aagggactgc ccgtacgcta gtgaagaaaa
     6961 gggtttctct gtctttcgcc aacggcgcac caatgtacat aatgcgtaac agattctcat
     7021 tagccggatt cagcacaaat agcggtggtg taataataaa atctgctgat tttttattat
     7081 ctggggtcat cacccatgac tgaataagat aaggaacatc cggactggtg ttacgtacac
     7141 ccagtgaaat ttgctcttca ccttctgaat aaacaagacg tgttgctgca agtccaatcc
     7201 cgcctttacc tgccgcccat gcggatgagc agctgagtaa tattgccgtt aaacttacgg
     7261 tcacaattcc ctttgttatg caagttttca taaatagtca ctcttaaaaa atatgaatat
     7321 cccctccaga acactagagg ggtactaatt aataaaacaa ttaatgagtt ctattaataa
     7381 tagttaatat ggaaatttgt taccgcattc acattaccag aagtcgcttt ccccgtagcg
     7441 atataatcaa ctttaaaggt cactgtttgc tcagcagaat ccgctaactg aatattcgac
     7501 gtttcggtac cgggagttac cgatgtagaa ccatctggca gatacatttg tagagcaaca
     7561 ttcgtcgctg catcggtggt agcctggttg gcaaatgctg taggttgcac gccgtcagcc
     7621 gtaccactaa aggtaaacgt cgcattttta gttacagtaa catcacagcc ttctaaggca
     7681 attggtacat cagttttaac gccagagact gctccattag tttgcaactt ggtggcgctg
     7741 acatttggca atgtgactac tgaatctttc ttcgcagcta ccagggtaca agctggagca
     7801 ataactttac cattaaatgt tactttgcca tccgtgatag cattatcatc agctgccata
     7861 acgctggacg ttgcacatac cacagttata aacgccgtca atacactttt tttcatcgtc
     7921 atcatcctga tctcttcgta aatatgataa tgcctttaaa tgacattaga cttaataata
     7981 acaataattg accgccattt aaatcaagca tactaaatcc aataagtgac aaatctttaa
     8041 tttatagatt tcatgaatat ttaaatatta actgaacgag taattcattg attcaacatc
     8101 taatgact
//
LOCUS       DAZFDP010000189         8099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFDP010000189 DAZFDP010000000
VERSION     DAZFDP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..660
                     /locus_tag="ACHJ1G_004465"
     CDS             <1..660
                     /locus_tag="ACHJ1G_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766201.1"
                     /translation="GEVIMRKLLLPLLFMAGTVNAASSVKEICTDYTKYLGHVYGFAV
                     SEDESMRKKLLSDMKRLKLSEAMVQQELYKVSTNANAKYQYSRLLNPDANEINRSTFD
                     YMVKACETAPDFAIPSWGVLVASNAVNKEDVGRNGIDSIRNAPGMRHQNVQGTLEERA
                     RGPGTNSPMGNLSPEELQEYNQRMEQYEKAAREQMEQQKNGSLNTFQQGLKALNLPYE
                     W"
     gene            722..1198
                     /locus_tag="ACHJ1G_004466"
     CDS             722..1198
                     /locus_tag="ACHJ1G_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766202.1"
                     /translation="MRIKRFFLILALLTPFSSMANVSKWSTGETHGVRSYAVSSKDNY
                     TLTFECDVGFNNTDPNQVGTRLLTLMKTEPGGESFDAKKEQITLKVGDDEYPISSIGS
                     SVGDSYWYGFWSDTPDMEVKTFDAYVDGKKIATFTLRKAAELFNAAPEDGCLKRAK"
     gene            1257..1823
                     /locus_tag="ACHJ1G_004467"
     CDS             1257..1823
                     /locus_tag="ACHJ1G_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766203.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITDQASKSLACPVDFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGDVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            1834..2445
                     /locus_tag="ACHJ1G_004468"
     CDS             1834..2445
                     /locus_tag="ACHJ1G_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766204.1"
                     /translation="MGFNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKGRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            2460..3341
                     /locus_tag="ACHJ1G_004469"
     CDS             2460..3341
                     /locus_tag="ACHJ1G_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HFO4766205.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDKKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            3423..6788
                     /locus_tag="ACHJ1G_004470"
     CDS             3423..6788
                     /locus_tag="ACHJ1G_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO4766206.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVAKAPSVDPIVDAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAVAGP
                     LWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQVNT
                     QKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGVASL
                     LMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAVGKG
                     ALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLDLGG
                     LVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTISTK
                     FDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIKNPG
                     KHIKEAASNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITPELK
                     APVNSQQGTSDSKAESDAKQTNIAARVINAALDMAKDSNKTVKETANQIINANAVETG
                     NKAAQTIDAALGQSATGKEEALSAYEIDKRRFNNGKDVSLPKLNAAGYQWISDNADYF
                     DELERKYGLEKGILSAVASAESSAGQRTGNPVDKNGNKLSSALGAFQITKGTREDLGL
                     SDADAMDTRKAADGAARYLSMLMNRYNGDQGRAIAAYHAGMGHVDKGRVVAGTGEYVT
                     RVRGYQQMLNNGAVYGSKVDHSAPAIHEKIPDNAVIDQSTGLAFTPGDSPFEKGGLVD
                     KIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVDTPMPVEEAR
                     PVANNSSYFDHLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAANDLQQPTGR
                     MQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEEHTKDSAKGQ
                     GTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            6788..7144
                     /locus_tag="ACHJ1G_004471"
     CDS             6788..7144
                     /locus_tag="ACHJ1G_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766207.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            7141..>8099
                     /locus_tag="ACHJ1G_004472"
     CDS             7141..>8099
                     /locus_tag="ACHJ1G_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFO4766208.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEG"
BASE COUNT         2300 a         1706 c         2106 g         1987 t
ORIGIN      
        1 ggtgaggtta taatgagaaa actattacta ccgttattat ttatggctgg gactgttaat
       61 gcagcatcaa gcgtaaagga gatttgtacc gattatacga aataccttgg gcacgtttac
      121 ggctttgctg tcagtgaaga cgaatccatg cgcaagaagt tactgtcaga tatgaagcgc
      181 cttaaacttt ctgaagcgat ggtgcagcag gaactgtata aagtctcaac caacgcaaat
      241 gctaaatacc aatattctcg cctattaaac cccgatgcaa atgagatcaa tcgaagcact
      301 ttcgattata tggtaaaggc atgcgaaacc gctcctgatt ttgctatccc tagctggggt
      361 gtgctggtgg cgagcaatgc cgttaataaa gaagatgttg gaagaaatgg cattgactca
      421 atcagaaatg ccccaggaat gcgccatcaa aacgtgcagg gtacgcttga agagcgggcc
      481 agggggccgg gtacaaactc cccaatggga aacctctcac cagaggaatt gcaagagtat
      541 aaccaacgga tggagcagta tgagaaagcg gcacgcgaac aaatggaaca acaaaagaac
      601 ggctcactta ataccttcca gcaaggttta aaagcgctta atttacccta tgaatggtaa
      661 atatattatg gataaccaat taaaatattg ataatcgtta atatccaaag gagataaatt
      721 aatgcgcatc aaacgatttt ttctaattct tgctttgctt actccatttt catcaatggc
      781 aaatgtaagc aaatggtcaa ccggcgagac tcatggtgtt cgttcttatg ctgtttccag
      841 caaagataat tatacgctta catttgagtg cgatgttgga tttaataata cggatcccaa
      901 tcaagtagga acacgactac tcactctcat gaaaacagaa cctggcggtg agtcatttga
      961 tgctaaaaaa gaacaaataa cgctgaaagt tggtgatgat gaatatccta tcagttctat
     1021 cggttcctct gtgggtgata gttactggta tggtttttgg tcagataccc ctgatatgga
     1081 agttaaaaca ttcgatgcat acgtagacgg aaaaaaaatc gcaacattta cgctacgtaa
     1141 ggccgcagag cttttcaacg cggcacctga agatggctgc ctgaagcgcg caaaatgacc
     1201 tgtcacaaat gactactcgt agaatcggtt aacacaccag attctacgag gtttcaatga
     1261 caccacgaca attactcgaa gacgtcaaat cccgcttcac acctttgatt gcggatgaac
     1321 ctgccttact ggaatccctg ctaagaaaag cattgggaac ctaccaggat agggcggggc
     1381 acatcaagcg gatacgcatc accgatcagg ccagtaaatc acttgcttgc ccagttgatt
     1441 ttcttgcgct cgtatcggtt acagatcaca ccggcgatct tgtctactcc gatgtttacg
     1501 atgggaatat cgagcttgaa gatacccatc gagcggtata cccactgaat gtgtcatatc
     1561 tggctaattt gcgtgatatg gatctggata atggggatgt gccacctgaa atcattgggt
     1621 tactttctga ctatctggaa gtgctaatcg cgatacctaa cactgatcgc ctgcgaagaa
     1681 tatctatcgc ggggaaactc gatgccagta atttatccga cgagaacacg ctgtatcagc
     1741 gaaagctgga tctggaagag aaaatgagcg caacaagggc aattatcccg ggaattgttc
     1801 ttttctcatc catgttgaag tgagagggct gatatggggt ttaatgttgc ttcagtaaag
     1861 tcttatgtat cttcggcatt aacgacgaca ttatttggct ccggcgttgg tgagcgggaa
     1921 gttggtaagc tgacgtcaat catcatgaac aaaatgctgt tcgcgcaagg atggcagttc
     1981 tctgtcgaag ttgatggtct ggagggggca gacttctttg ccaaagacat tacctaccac
     2041 gattacagca tcgaatatga aacgattaaa ataggcggag ggaatatcct tcagccaacg
     2101 gagcgttcgc ctgggcagat aacaatgatg gtcagggata ccgttgatgg cctcgttttg
     2161 gactggttta agacggcaaa aggtcgggtg atcaatccgg acggtaccgg gaatataccg
     2221 tctaaatatt tgctcaatgt gcgtatttat cggttgctgt cctccggttt aaccaaactg
     2281 gaaaatgaga tgacggtatt cccggtcact accggcgatg tcacctatgc gcgggatcag
     2341 gttacggaat ttaagtcatt cccaatgacc ttcgcattgc acagcacgtt taaccaatcc
     2401 tcaagttctt tagcttccct tctgggcttt agtttttctc tttgaattaa ggagcaagga
     2461 tgcttttacc tcttttcccg ctaccatcgc ggccaactga attgatccag ttccgtcagc
     2521 caaatattgc tgatgcgatg cgtttcaact cgataacacc ggaggaacaa gaacaacaga
     2581 caacggcgta tttaaaagcc ttgctggctg aacccgcgaa atatgatccc ctgacatgga
     2641 cggcgcagga ccggattacc gcgttatggt ggatatttac tggctcccgt gaaacaccgg
     2701 tcgagacatt cacctacacc tgtaaacatt gcggtaaaga gcattattac gattgcgata
     2761 tgaatgctct ggctgaagat atccaggtcc tggaagtgga accgttcatt gacgatattg
     2821 aggtgtctgt agagggcgtg ccttatcaat ggcgtatcgt gccgcttgat ggttgggcaa
     2881 tggaaatgct ggagatgcgc cgtgcagcat tgccacctga agacgacgcg gaattcaaag
     2941 aagcgatcgt tgatttgcgt ttttgggaat tcgcttatca gtgtgagctt tataacgatg
     3001 ttagcggtac tcgtgaagat caggctgagc gtcgttatga aacgattaaa cggatggcca
     3061 ttgatactga atttatgaag ctggcggcac acatccgact ggctcatgaa aagctcgaac
     3121 atggtttacc gtgctacatc gataaaggtg aaatgcgtct tcgtctcccg ccgcataaat
     3181 gcccaaatca ggataaaaag gagtccacag agggtgcata tacccgtctg tgggtgccct
     3241 ttcgggctac cgacttcatt ccacaggtgg ggattgaaaa gctatcagac cttagtgtcc
     3301 aacctggttt tgtatggggg tataccgatt caggacgctg aaaggcttac tgaatcctat
     3361 gcgtttttcc tgttggagaa actggaagaa aaacttaaac cgaaacggta ggcgataaga
     3421 tcatggaaag aaaaaacgcc aacattgacg atgtgataag gacagttgaa accgccagcg
     3481 caaaagagct ggaggagctt gctggtatcc gggaagctgt tgaagatttg aaaggtgggc
     3541 gagttgctac tgttgatcct gtctctcgca gcgtgtcggc attaaatcgc acaatcgaaa
     3601 attcccggcc tgactttgtg gccaaagcgc catccgtaga ccctattgtt gacgcaatga
     3661 aacggcttaa tttaggggac gtttctcgtg tagttcagga ggatgttgct ctacaggaac
     3721 cgcaggccaa atcaactacg cgaaagggta aaaaacgacg caagaaggct ataacagaag
     3781 atgtaaaggc gcaacggacc gaagcagccg aacacgctcg cgaaatgttc ggtcaaaaag
     3841 gcggtgcgca aaaaagccaa aaccaacgcg atgcgcgtgg tcgttttatt ggaaagtcag
     3901 ggagtaaggc cgcagcggaa gatgcccgtg ctgaaaaggc caggcgcaaa gaggatgatg
     3961 agcgtctaaa tgctgaatca ggtttattaa aaaaactgtc aaaagtagct gaaggcatag
     4021 gtaacccttc agagactcgt gccgtcgatg cgttaggtta tgccgttgct ggtccattgt
     4081 gggccgcagg gaaggagctt ggcgggatat caaaagaagt tggtggatcg cttaatggtg
     4141 ccagaaagtc tattgccgat gtgattcgtg gcaatgacga taacagccgt agaaaaggtt
     4201 tttttaggcg taaatcgcaa aatagtgccg atgtcgttca ggttaacacc caaaaacgga
     4261 cggttcagga acttcaggat cagaccagcg aaattaaaga gggcaatgac aagattctca
     4321 gcgcccttga tcagatagcc aaaaacaccg ggaaaaagaa gggcggcttg ctgtccaaat
     4381 tatttagcct gttagggaag ggggccggtg gcgtcgcgtc gttgttaatg gggcgtggca
     4441 tgctgaaaaa agctggagca ctcgcttttg gcgctctggg ggcaaagaaa cttgtaggaa
     4501 tgctacgcgg tggtggcaag aagactctcg cccatgaagg cggagatttg gctgcccggg
     4561 cagcaggtaa acttggatta aaggcagttg gtaaaggggc gttacgcgca attcccctag
     4621 tcggcacagt ggctggaggt atttatgatg cggtaaccgg ttggaatgat acagaagcgc
     4681 aacgtcgagc gtttgggctt aaatcaggac aagatccatc attccagcaa aaagccgctt
     4741 atacgttagc caatgttctt gatttggggg ggctggtatc tggtattagc agtgctattg
     4801 gtgatgttct caaatcactt ggatttgagg atatcggcaa tatgttgcaa tcattttcga
     4861 cggaaagtat tgcccaggcc attgatagtg ggattaccaa cttagaaaca tatatttcta
     4921 accttggcga caccatttct accaagttcg atgattacac agcaaagatt ggtgatgctg
     4981 tttcagcatg gtttagcgat acatctaata agctgcttga aaagctggat gccatcaaag
     5041 acttctttac tgtcgataac ctgaaacagg ttttcagtga tgcaattgat agtgcaattg
     5101 atttcattaa gaacccaggg aaacacatta aagaggcagc tagtaatatt tgggatgggg
     5161 ttaaaaattt accaggtaaa gcattagatg cagcggttga tgccgttaaa aatacccctg
     5221 cggcaatgat tgtatcaaaa atacccaatc cgatcggcga ggctaatgcg aaagaaatca
     5281 ctccagagtt aaaagctccg gttaatagcc agcaggggac atctgattct aaagctgaat
     5341 ccgatgccaa acagactaat attgctgccc gcgtgataaa tgcggccctg gatatggcga
     5401 aagatagcaa taaaacagtt aaagaaactg ctaatcagat tatcaatgca aatgccgtag
     5461 aaacaggaaa taaagcagca caaacaattg atgctgcctt gggccagtct gctacaggta
     5521 aggaggaagc attaagtgca tatgagatag ataaacgtcg atttaacaat ggcaaggatg
     5581 tttctttgcc aaaattaaat gctgccggat accaatggat ttctgacaat gccgattatt
     5641 ttgatgagct tgaacgtaag tatgggcttg aaaaagggat tctgtcagca gttgcatccg
     5701 cagagtctag tgcaggccag agaactggaa atccagtaga caaaaacggg aacaaacttt
     5761 catctgccct tggggctttt cagatcacta aaggtacaag ggaggatctt ggactcagcg
     5821 atgctgatgc catggataca cgaaaagcag ctgatggtgc cgccagatac ctaagtatgc
     5881 tgatgaaccg ttataacggt gatcagggtc gtgcaatagc tgcctatcat gctggtatgg
     5941 ggcatgttga taaggggaga gtagtcgccg gtaccggcga atatgttact cgtgtcagag
     6001 ggtatcagca gatgctcaat aatggtgccg tttatggctc taaggtagat catagcgcac
     6061 cagcaattca cgaaaagata cctgataacg ctgttatcga tcagtctact ggcctggcgt
     6121 ttacccctgg tgatagcccg tttgagaaag gcggtctggt agacaaaatc ggcaatgctg
     6181 ttggcgttaa cgatctggtc aacaaattca tgaatggccg ggggatgcgt cgggaagtcg
     6241 ttcagggaac gctagaagaa cgtgcacgag ggaaggggac cgcaacagca gctggcaatg
     6301 tgtatgttga tactccgatg ccagttgaag aggcgcgtcc ggtggccaac aactcaagtt
     6361 actttgacca tctcggcgca caaatgggga ttgatggact attcgataaa ctccgcaact
     6421 cgccggggat gcggaaaaat aatgcgcctg aaccagcctc cacgtcccag gtgacgactg
     6481 ccgccaacga tttgcagcaa ccaaccggtc gtatgcagat agacgggcag gttattagtg
     6541 accttggcgg ttccggtgcc aagccgacaa tgcagttggc tgataatacc gtttcacttg
     6601 atggtgaaac gaagcggctg tttgcgcaga tgacctcatt gcttgccagg attgaagagc
     6661 acactaaaga ctcggcgaaa ggccagggaa ctgtcgtaaa ggtcagcacg cctcagccgg
     6721 gcgttatgcg cacggtacca ctgtcaattg atgatccgtt gatgaatgac tacgcgagag
     6781 ttgattgatg gccaacaata acgaaattga tcctttgctg acgctggagt tatccggcgt
     6841 aaaaacgtat gagtcccagg aggaggcctg gggcgctcgt ttatatgagt ggctaaacac
     6901 ttatcagggt gaggtatacg gagatccgtc atggggcaat gttttaccgc agtttaaaca
     6961 cgaaccgacc aacttgtcgc atgttcaaat tgcggttgag gcaatgctgt tgcaaaaact
     7021 gacggtagat ttacctgaca taccgatttc tggcttgtca gtagccgagg gagatgcttt
     7081 tgataagttg aaaatatcca ttcgtatcag ggatataact atcacacagg acgtggtgct
     7141 atgagtaaaa caacaccgac taaagacagt attcgtgcag agtttgaaga gcttgtcgag
     7201 aaagattcat tctggtcgaa gtttgtcggc tctcaatttg tctcgatgct gacattgttt
     7261 attacccaga ttgtctacag gtgctttcag tatgccgatg cggcgctggc tgaaggcttt
     7321 atatcgaccg cgacgcggcg ttcctctatc ctggcagcgg cagaaacgaa tagttacgtt
     7381 ggtaccaagc caacaccgtc atcggggatg attgagatca ccgccacaag tgaagatgcc
     7441 ccagcggtaa tccccaaaaa catgccttta atatctgacg accagtaccc ttacatgact
     7501 atggatgtat gcaggttggt tgacggcacc ggtacggtag aagtggcaca gttggaaatc
     7561 caggaggtga catataccgt tacggcagcc aaagaatttc tggaagtcgt gttatcaaag
     7621 gctctcactg ctgtctgcta taagctggaa gtattcgtga cgaccgatgg taagaccacg
     7681 cagtggtctt ccagcactat gttccggtta gccggtagta aaagccaggt ctacgttgag
     7741 ttttataagc catccgagca gttgggggtt cgattcggtg atgggctaat tgggcaaata
     7801 ccgccagaag gctcgaccat tacacttaag gtatggtgca ccaacggaga tataaccctg
     7861 gttgctggcc aaaatctgac gcctgtcgat tctgcggcta atttagctaa tttgatttca
     7921 gttaagacaa cgacacccat aaccgcaggt accgatgccg aaacaacgga gatcacacgt
     7981 aatcgtgcac aatattacct tgcctatgat gatcaggtcg tatggggcgg ggactatacg
     8041 tattttctgg tgcgtaacat cccgggactg tcctgggtaa aggcatgggg cgaaggcca
//
LOCUS       DAZFDP010000190         7979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFDP010000190 DAZFDP010000000
VERSION     DAZFDP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1170
                     /locus_tag="ACHJ1G_004473"
                     /pseudo
     CDS             1..1170
                     /locus_tag="ACHJ1G_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1139..2875)
                     /gene="aceK"
                     /locus_tag="ACHJ1G_004474"
     CDS             complement(1139..2875)
                     /gene="aceK"
                     /locus_tag="ACHJ1G_004474"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4766209.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3156..4460)
                     /gene="aceA"
                     /locus_tag="ACHJ1G_004475"
     CDS             complement(3156..4460)
                     /gene="aceA"
                     /locus_tag="ACHJ1G_004475"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4766210.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4490..6091)
                     /gene="aceB"
                     /locus_tag="ACHJ1G_004476"
     CDS             complement(4490..6091)
                     /gene="aceB"
                     /locus_tag="ACHJ1G_004476"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4766211.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6360..7289)
                     /gene="metA"
                     /locus_tag="ACHJ1G_004477"
     CDS             complement(6360..7289)
                     /gene="metA"
                     /locus_tag="ACHJ1G_004477"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4766212.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7446..7889
                     /locus_tag="ACHJ1G_004478"
     CDS             7446..7889
                     /locus_tag="ACHJ1G_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4766213.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2006 a         1994 c         2001 g         1978 t
ORIGIN      
        1 gaattaaact taatggtcaa tgttattttc cggggcgacc ccaaaaccgt atagtatgcc
       61 gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc
      121 aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc
      181 agactttggc gttgatttcc agccgtcctg aggggcgttg tgttgctgcc tgtggtgatt
      241 tcgggctagt tatgaaggca tatttcgaca agatggaatc aaatggcatc agtgttatgg
      301 cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata aagaacacaa
      361 ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtcact aacgataaaa
      421 taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg gattttatga
      481 atgtagatta tagcctcctg aaatggtcaa atgatcatat tattaaccaa tctgttgcaa
      541 taattccagc acttccgaaa gaacagctat tgatgttaaa aggatctgtg gatgaaataa
      601 cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag aatcaccaac
      661 ttacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca gaaatgttga
      721 ctgcctataa tagtggacat atgaacgtta ttaatactat ttttaacgca taacccactc
      781 tgtttaatac gtttaaattc gataaaaaaa aatatgaagc ccctcctcct ggcaaataat
      841 tctaatgaat atcccggttt gttttcagcg atacagcata aacaacaaaa tgttgtagag
      901 acggtttatc ttgctttatc taaccatgca cgcctgtttg gatttaccgc tgaagatatt
      961 atggattttt ggcaacacaa agccccacaa aaatactctg cctttgagtt ggcttttgaa
     1021 ttgggtcacc gggttattgc tgaattaatc cttaatacat taaataagat ggctgaaagc
     1081 tttggcttta cggataaccc tcgatacatt gcggagaaaa attatatgga agctttactc
     1141 aaaaaagcat ctccccatac cgtacgctaa atctttgcct gcgccgatac gcataaacat
     1201 cttccacatg cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca
     1261 ggtcggcgtg catctcttca aacagcggac caatacgcgg gtcggcgcat agccagtggc
     1321 gaaactcttc cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt
     1381 cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa
     1441 tttcatcgta atcataaaaa accacacgcc cgtgacgggt gacaccgaag tttttaaaca
     1501 gcatgtcgcc aggaaaaatg ttagcagcgg caagctggcg aatagcgtta ccgtattctt
     1561 caatggcgtc gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca
     1621 tccgccgctc gatatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
     1681 ccgctgcttc ctggagcagt aattccatta atgccgggga aatatgccgc ttctccagca
     1741 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
     1801 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
     1861 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa taccaacatc accattccac
     1921 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
     1981 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
     2041 ttttacccgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
     2101 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
     2161 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
     2221 tgccggaagg cgtgatcagt ttgcccacca gccaagcggc tttattgcgg taaaacagtt
     2281 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
     2341 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
     2401 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
     2461 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
     2521 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
     2581 caatctcgaa gtgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
     2641 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
     2701 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
     2761 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
     2821 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
     2881 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
     2941 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
     3001 tgccggatgc ggcgtaaatg ccttatccgg cctacgttcg gcaacggctg taggcatgat
     3061 aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtgaacgcc
     3121 ttatccggcc tacagtcagc aacggttgtt gttgcttaga actgcgattc ttcagtggag
     3181 ccggtcagcg cggtgactga agacgtgccg ccctgaataa tagtcgtcac tttatcgaag
     3241 taacctgtac ccacttcctg ctggtgagat acgaaggtat agccatcttt cgcggcggca
     3301 aattccggct gctgcacttt ctcaacgtag tgcttcatac cctcgccctg ggcataggcg
     3361 tttgccaggt caaacatgtt gaaccacatg ctgtggatac ctgccagggt gatgaactgg
     3421 aacttgtagc ccatatccga cagctgctgc tggaagctgg caatagtttt gtcgtcgagg
     3481 tttttctgcc agttgaacga cggcgagcag ttataagcca gcagtttgcc cggatatttc
     3541 gcgtggatag cttgtgcaaa gcgacgcgcc agttccagat ccggcgtgga ggtttcacac
     3601 cagaccaggt cagcatatgg cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc
     3661 gcatgagtac ggaagaagcc ttcactggta cgctcgccag taataaattc actgtcatac
     3721 gggtcgcaat cggaggtaat cagatccgcc gcatcggcat cggtacgagc aaccagcagg
     3781 gttggaacgc ccgtcacgtc agctgcaaga cgcgccgcga ccagtttctg aatagcttcc
     3841 tgagttggca ccaaaacttt gccgcccatg tgaccgcatt tcttcactga cgccagctga
     3901 tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca
     3961 ttcaggacac cgccaaaacc ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca
     4021 tagcgcggat cgcccggctc aatgcctgcg gaccattgga tctgatcggc acgacggaag
     4081 gtgttgttga tccgctccac cacagctggc accgagtttg ccggatagag cgactgatcc
     4141 ggatacatgc tggccgccag gttagcgtcc gccgctacct gccatcccga cagatagact
     4201 gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg
     4261 ttgatgtagc cttttttcga ctcgccatgc agcagacgcc acattttcgc cgcgcccagt
     4321 tgcgccaggg tgcattcagg attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg
     4381 tatgggcgag taatgccttc ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt
     4441 tgttgtgtac gggttttcat gtgcagatgc tccatagtta tgtggtggtt tacgctaaca
     4501 ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg atctgttcca
     4561 tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg ttcttcgccc agttcgctgg
     4621 caatgacttt catctcttca cccagcatct ggcggaacaa ggctttggtc accggtttgc
     4681 cattgctcaa cgttttttga tgatggatcc actgccagat tgaggtacgg gaaatttcag
     4741 ccgtcgccgc atcttccatc aggccataaa tcggcacgca gccgttgccg gagatccatg
     4801 cttcgatgta ctgcactgcc acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac
     4861 catcacaagg tgccagcagc tgatcggcag taatcggcgc gtcttgttcg cgcatcactt
     4921 caagctgatt tttacgggag ccgagaatgt cgttgaatac cgccattgcc gtatccgcaa
     4981 ggcctgggtg agcaatccat gtgccatcgt gaccgttatt ggcttccagc gctttatccg
     5041 cttttacttt gttgagcacc tggttattgc gctcttcatc tttgctcgga ataaacgctg
     5101 ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt
     5161 aagcattcag gaatggttta tccatcgtca ctgcctgtct gtctggcagg acgcgatcgg
     5221 gatagttttt caacgttttg atatagctga agatgtaatc ccaacgaccg cagttcagac
     5281 caacaatatg gtcacgcagc gcgtgaagga tttcatccat ctggaacacg gcgggcagcg
     5341 tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga tcttctgcat
     5401 agctgaagac ttcgctccac caggccgctt cctgccagga ctgggttttc ggcagataga
     5461 aatagggacc gctgcccttt gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa
     5521 aatcaaacag gctaccgggg attgcctcac cacgccaggt gacatgtttt tccggcaagt
     5581 gcagaccgcg tacccgacaa atcaaaaccg ctggattggg cttgagctga taaattttgc
     5641 ctgcttcatt ggtatagctg atggtgccgt taaccgcatc gcgcaggtta atttgcccgt
     5701 cgatcacttt gttccagtct ggtgccagtg aatcttcgaa atcggccata aagactttca
     5761 cattggcgtt cagtgcgttg atcaccatct tgcgctcaac cgggccagtt atctcgactc
     5821 gacgatcttg taagtccgcg ggaataccac gaattttcca gtccgtatcg cgaatggaag
     5881 ctgtttccga aataaaatca ggcaaagttc cgttatcaat atcttgctgc tgctgaatgc
     5941 gcgctgccag aagtttattg cgttgtggcg taaaatgcgt caccagctca gtcagaaatt
     6001 ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc atacggcctt atgaaagcca
     6061 gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg gacccgaaaa
     6121 cttccccact gtgaacgaag gatcgttgtg cgcttttcgt tcagcacaac taagactact
     6181 catttaaaat gcaaaatcaa aaacaatttc cattttaatt aaaaatactt gataacttat
     6241 tgataacaat atgaataaaa tttattcacc tcctgaggtg cgtttcggaa ataaaaaagg
     6301 cacccgaagg tgcctgaggt aaggtgctga atcgcttaac gatcgactat cacagaagat
     6361 taatccagcg ttggattcat gtgccgtaga tcgtatggcg tgatctgata gacgtaatag
     6421 ttgagccagt tggtaaacag caaattaccg tgactacgcc agctcgctcg cggtgtattt
     6481 tgcggatcat tgtgcgggaa atagttatac ggtacttccg ggcctagtcc ggcttccaca
     6541 tcgcggaaat attcctgcgc cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag
     6601 gcaatgcgct tatctttgct ggcaaacaga tatgcatccc cttcttccgt ctctgccaga
     6661 atttccagat cggtgtaatc acgaatcaac gctgccggaa agtcagcata gcgcgaatgt
     6721 ggtgccagga atgaatcatc aaagccacgc gtcagaagcg catgaggatg gagaatatga
     6781 tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct taggaatgcc gtagaggata
     6841 ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg aggtgacgtg atctttcgac
     6901 cactccagca cctgtttgat ctgcggccag taagcgacat cattaaactc caccaggccc
     6961 agcggcgcac cggttacaat cagaccgtca aagttctgtt cctgaatatc ttcaaagtta
     7021 cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc
     7081 aacagctgaa tatcgacctg caaaggtgag tttgaaagca ggcgcagaaa ctgattttca
     7141 gtttcaatct tcttcggcat caggttaagg attagaacct taagtggacg aatttcctga
     7201 ccagacgcac gagaagttgt catcacaaag acgttttctt cacgcaagaa attgacggcg
     7261 ggtagctcgt ccggcacacg aataggcata acctgattac ctcactacat acgcttatac
     7321 gtttagacat ccagatagct gaagataacc agaaaatttg ccaatgtcga gcctgcatgt
     7381 tgaaggtgag aaagtttcaa ggaaaaggct acgttagaat ataagaatga cgaaaaggag
     7441 agaaaatggt tattagtatt cgccgctcac agcatgagga aggggaggaa ctcgttgcga
     7501 tttggtgtcg atctgtcgat gccacccacg attttttatc agcagagtat cgggccgagc
     7561 tggaagagct ggttcgttcc tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac
     7621 gggatcagcc ggttgggttt atgttgttaa gtgggcagca tatggatgcg ctgtttatcg
     7681 atcctgatgt gcgcggctgc ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg
     7741 caccggaact gacaaccaac gttaatgagc aaaatgagca ggcggttggg ttctataaga
     7801 aggtgggttt taaggttacg ggacgctctg aggtggacga tttggggaaa ccgtatccgt
     7861 tgctgaatct ggcgtatgtg ggggaataag ccgcccctgc cgtgcctgcc caggctggag
     7921 aagacgcgcc agcgtcgcac ccggcatttt tttcaccaac gcaaaaaggc catcgtgac
//
LOCUS       DAZFDP010000191         7974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFDP010000191 DAZFDP010000000
VERSION     DAZFDP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHJ1G_004479"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHJ1G_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4766214.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHJ1G_004480"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHJ1G_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4766215.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHJ1G_004481"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHJ1G_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4766216.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHJ1G_004482"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHJ1G_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4766217.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHJ1G_004483"
     CDS             7014..7292
                     /locus_tag="ACHJ1G_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766218.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHJ1G_004484"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHJ1G_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4766219.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2067 a         1855 c         1884 g         2168 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctatt ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtatcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga ttta
//
LOCUS       DAZFDP010000192         7945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFDP010000192 DAZFDP010000000
VERSION     DAZFDP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            238..1266
                     /gene="torT"
                     /locus_tag="ACHJ1G_004485"
     CDS             238..1266
                     /gene="torT"
                     /locus_tag="ACHJ1G_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4766220.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1239..1931)
                     /gene="torR"
                     /locus_tag="ACHJ1G_004486"
     CDS             complement(1239..1931)
                     /gene="torR"
                     /locus_tag="ACHJ1G_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4766221.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2071..3243
                     /gene="torC"
                     /locus_tag="ACHJ1G_004487"
     CDS             2071..3243
                     /gene="torC"
                     /locus_tag="ACHJ1G_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4766222.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3243..5789
                     /gene="torA"
                     /locus_tag="ACHJ1G_004488"
     CDS             3243..5789
                     /gene="torA"
                     /locus_tag="ACHJ1G_004488"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4766223.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5786..6385
                     /gene="torD"
                     /locus_tag="ACHJ1G_004489"
     CDS             5786..6385
                     /gene="torD"
                     /locus_tag="ACHJ1G_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4766224.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(6478..6783)
                     /gene="cbpM"
                     /locus_tag="ACHJ1G_004490"
     CDS             complement(6478..6783)
                     /gene="cbpM"
                     /locus_tag="ACHJ1G_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4766225.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(6783..7703)
                     /gene="cbpA"
                     /locus_tag="ACHJ1G_004491"
     CDS             complement(6783..7703)
                     /gene="cbpA"
                     /locus_tag="ACHJ1G_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4766226.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1905 a         1985 c         2171 g         1884 t
ORIGIN      
        1 aaaatcacct tgcgctaatg ctctgttgta ggtcactaac cacatctaac tcattgatta
       61 atagtgactc tagatgtagt gttttatcgt attttgcagt ctgttattta atcatgaatt
      121 catgtaacaa tatgattttt aaaatatttg tgtgtatttg tcatccttta ggtgaaaaag
      181 gttgagtcgc aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg
      241 cgcgtactgc tatttttact tctttccctt ttcatgttat cggcattttc ggctgataac
      301 ctgttgcgct ggcatgatgc gcagcattac acggtgcaag cctctacgcc gcttaaagcc
      361 aaacgcgcat ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg
      421 ttgaactatg gtatgcagga ggcagctcgc cgctatggtg tggatttaaa agtgctggag
      481 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
      541 ggcgcagagg ccattttgct cggtagtagc actacgtcat ttcccgacct gcaaaagcag
      601 gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccct ggtgaaaagc
      661 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
      721 gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataatgc cgggggcagt
      781 aaagagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
      841 attgcgcttg gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa
      901 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
      961 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
     1021 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
     1081 ggggaactgg cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
     1141 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc
     1201 tcgctgtcac cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
     1261 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
     1321 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
     1381 cgtagcagac gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt
     1441 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
     1501 acattcaggc aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt
     1561 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
     1621 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
     1681 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
     1741 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
     1801 ttctgcataa tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc
     1861 tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
     1921 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatc tgttcatatt
     1981 ctgccgtaag ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct
     2041 tcactaaacc ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga
     2101 cccagtgctc gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg
     2161 ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt
     2221 tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc
     2281 tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg
     2341 aagcgcaaac tggaagcgag caatgatatc taccagacct ttattgctca ctccattgat
     2401 acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg
     2461 aaagaaaaca actcggcaac ctgccgctcc tgccataact acgatgcgat ggatcatgcg
     2521 aagcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc
     2581 atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag
     2641 cagttcgatg agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat
     2701 attaagccaa tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct
     2761 tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg
     2821 acggaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt
     2881 gcctcgattc gtggtgatgt gcagcagcag gtaaaaacgc tggagaaaac caccgttgcc
     2941 gacaccaata ccgagtggag caagttgcag gccactgcgt ggatgaagaa aggcgacatg
     3001 gtgaacgata tcaaaccgat ctgggcttat gcggattcgt tgtacaacgg cacctgtaac
     3061 cagtgccacg gtgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc
     3121 aacggcatga ttggctttac cagtctcgat aaacgtgaag aacgcacctt gttgaaatat
     3181 ctgcaaatga atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa
     3241 taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg
     3301 gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcgactgcgg
     3361 cgcaagcggc gactgacgct gtcatctcga aagagggcat tcttaccggg tcgcactggg
     3421 gggctatccg cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg
     3481 ataaatatcc gtcgaaaatg attgccggat tgccggatca cgtacacaac gcggcgcgta
     3541 ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg ccatctcagc gatacctccc
     3601 agcgcggtga taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg
     3661 aagaactgga acgcgtgcag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt
     3721 ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc
     3781 atggtaatag cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc
     3841 cgcgcgtggt tggctcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc
     3901 agaacagcaa aaccattgtg ctgtggggct ctgatttgct gaaaaaccag caagcgaact
     3961 ggtggtgccc ggatcacgat gtttatgaat attacgcgca gctaaaagcg aaagtcgccg
     4021 ccggtgaaat tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg
     4081 ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgatgtgccg ctgcaactgg
     4141 cgctggcgta tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact
     4201 gtgtgggttt tgagcagttc ctgccgtacc tgctgggtga gaaagacggt cagccgaaag
     4261 atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc
     4321 ggcagatggc ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc
     4381 acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc
     4441 tgccaggtgg tggctttggt tttggctggc actataacgg cgcaggcacg ccggggcgta
     4501 aaggcgttat tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca
     4561 gtgattacaa aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg
     4621 aaccggggaa agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt
     4681 gtatttttgc cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag
     4741 gctggcgcaa gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct
     4801 ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg
     4861 gcaaccactc caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg
     4921 cgcgcaacga ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct
     4981 ttaccgaagg gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac
     5041 aaggcaaagg acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt
     5101 acgtcgagtt tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg
     5161 atctcgaacc gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg
     5221 atatgaacta cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc
     5281 acggtgggcc cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc
     5341 gacttcactc gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta
     5401 aagagccagt gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg
     5461 tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct
     5521 atgcacccgg cgtggcacga attcacgaag gggcatggta cgatccagat aaaggcggcg
     5581 agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt
     5641 cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg
     5701 gagcagtgga gcaggtgacg gcgtttaacg gccctgtgga gatggtggcg cagtgcgaat
     5761 atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt
     5821 ttacgcctgg ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat
     5881 cgccagtgcg cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctggctgc
     5941 ggcggtgaat gagctggaaa actgtattgc cacgctgaca gtacgtgacg atgcccgtct
     6001 ggaactggcc gctgactttt gcggcctgtt tctgatgacc gacaaacaag cggcgctgcc
     6061 gtatgcatcg gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg
     6121 gatggaaacc agcggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgaatt
     6181 gctcagccat ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag
     6241 tttgcggcaa aaaacactga cggcgctgtg gcaatggtta ccagagtttg ttgtgcgttg
     6301 tcgtcagtat gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt
     6361 ggagagcgac caccaaaaca gataacgtcg tttgtgcgcc tgaaaagacg cgtttagcgt
     6421 cgcatcaggc attatggggc agttgccgga tgcgtcatca catccggcaa acacacatca
     6481 cggatgagtt acaaaccggg aaagtcgctg gcgcagcagg cggttttcct gcttcaggtg
     6541 cgcaatatca tccattaacg tcagcgccac cgcgatccca ggccagtcca gagccagttc
     6601 atgacgcagg cgtactgcgc gttgcaccac gatggcggca tggtcgtcaa atacccaggt
     6661 tgtttcctgg atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc
     6721 ttcagagatg ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc
     6781 cattatgctt tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc
     6841 tgccacagcg cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca
     6901 tacagatcgc cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc
     6961 ccggcctggc tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg
     7021 actttagcac ccagcgccgc ttcccacggg ctaaccggca ctacaatttc cagatcatgg
     7081 ccgacaatat cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt
     7141 ggaccgccgt tttcgcccgg cgtcccctgg cctttcagac ggatgcgttg accattgccg
     7201 acgcccgccg ggatcttgac attcagcgtt ttcggaattt cctgttcgat catgccaaag
     7261 gcgttataaa ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg
     7321 aggaataccg ccacttcgat ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc
     7381 tggcgggcat gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcgttaaaa
     7441 ctctgaccgt cgctatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac
     7501 atctgatcat actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc
     7561 tctttgaagc gggcttcggc atccggttct ttgctgacat caggatggta tttgcgggcc
     7621 agtcgacgat aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg
     7681 gcgtaataat cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa
     7741 cccctgtaag gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca
     7801 gaatatgtta ttgaaatcat cgcggagagg aggtcgcctc aaggtggggt gctgaacata
     7861 ttttaaacag gtgaaaaagg gtgagcgatt tttgatagtg gaaccaggca ctttaagttt
     7921 aactggggcg tcattattta ttaaa
//
LOCUS       DAZFDP010000193         7902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFDP010000193 DAZFDP010000000
VERSION     DAZFDP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1571
                     /locus_tag="ACHJ1G_004492"
     CDS             300..1571
                     /locus_tag="ACHJ1G_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4766227.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHJ1G_004493"
     CDS             complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHJ1G_004493"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4766228.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHJ1G_004494"
     CDS             complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHJ1G_004494"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4766229.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHJ1G_004495"
     CDS             complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHJ1G_004495"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4766230.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHJ1G_004496"
     CDS             complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHJ1G_004496"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4766231.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHJ1G_004497"
     CDS             complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHJ1G_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4766232.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7637..>7902)
                     /locus_tag="ACHJ1G_004498"
     CDS             complement(7637..>7902)
                     /locus_tag="ACHJ1G_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766233.1"
                     /translation="GTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVTETIVFF
                     YICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1818 a         2138 c         2024 g         1922 t
ORIGIN      
        1 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
       61 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
      121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
      181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
      241 gcaagagaaa ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta
      301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
      361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
      421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
      481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
      541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
      601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
      661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
      721 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
      781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
      841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
      901 gcgatgtcct gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta
      961 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
     1021 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
     1081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
     1141 cggcactggc gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg
     1201 tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
     1261 ctattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
     1321 cacgcatgct gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt
     1381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
     1441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
     1501 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
     1561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
     1621 cctgatcaac agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca
     1681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
     1741 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
     1801 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
     1861 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
     1921 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
     1981 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
     2041 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
     2101 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
     2161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
     2221 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
     2281 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
     2341 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
     2401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
     2461 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
     2521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
     2581 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
     2641 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
     2701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
     2761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
     2821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
     2881 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
     2941 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
     3001 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
     3061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
     3121 ggaaagctga tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt
     3181 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
     3241 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
     3301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
     3361 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
     3421 ccccataatc gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac
     3481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
     3541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
     3601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
     3661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
     3721 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
     3781 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
     3841 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
     3901 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
     3961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
     4021 acgggccgaa gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
     4081 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
     4141 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
     4201 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
     4261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
     4321 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
     4381 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
     4441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
     4501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
     4561 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
     4621 agtcgcggcg ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg
     4681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
     4741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
     4801 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
     4861 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
     4921 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
     4981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
     5041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
     5101 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
     5161 ccgcagccag cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc
     5221 caagttccag cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt
     5281 tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc
     5341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc
     5401 cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
     5461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
     5521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
     5581 cacaaatcaa ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg
     5641 cacgcagagg cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga
     5701 agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa
     5761 acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt
     5821 gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat
     5881 cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc
     5941 tgaacagggt ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta
     6001 ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa
     6061 ttttggcctg cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag
     6121 cgttcgggtt atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt
     6181 ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa
     6241 cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc
     6301 cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa
     6361 ctttcacgtc atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc
     6421 ccggcatttc catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt
     6481 tcgggtacgg catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt
     6541 caggagtaat agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa
     6601 acgctttgta gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc
     6661 cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt
     6721 cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc
     6781 gagtcagcac aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg
     6841 gcaagcccat gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt
     6901 gcgcgttttt ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg
     6961 gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt
     7021 taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga
     7081 ttttaaattc aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg
     7141 aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt
     7201 tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc
     7261 ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac
     7321 cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt
     7381 tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa
     7441 tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg
     7501 atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg
     7561 cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac
     7621 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
     7681 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
     7741 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
     7801 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
     7861 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gt
//
LOCUS       DAZFDP010000194         7890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFDP010000194 DAZFDP010000000
VERSION     DAZFDP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(275..760)
                     /gene="sixA"
                     /locus_tag="ACHJ1G_004499"
     CDS             complement(275..760)
                     /gene="sixA"
                     /locus_tag="ACHJ1G_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4766234.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(963..3107)
                     /gene="fadJ"
                     /locus_tag="ACHJ1G_004500"
     CDS             complement(963..3107)
                     /gene="fadJ"
                     /locus_tag="ACHJ1G_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4766235.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3107..4417)
                     /gene="fadI"
                     /locus_tag="ACHJ1G_004501"
     CDS             complement(3107..4417)
                     /gene="fadI"
                     /locus_tag="ACHJ1G_004501"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4766236.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4597..4881)
                     /locus_tag="ACHJ1G_004502"
     CDS             complement(4597..4881)
                     /locus_tag="ACHJ1G_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4766237.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5253..6593
                     /gene="fadL"
                     /locus_tag="ACHJ1G_004503"
     CDS             5253..6593
                     /gene="fadL"
                     /locus_tag="ACHJ1G_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4766238.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6958..>7890
                     /gene="yfdF"
                     /locus_tag="ACHJ1G_004504"
     CDS             6958..>7890
                     /gene="yfdF"
                     /locus_tag="ACHJ1G_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO4766239.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPE"
BASE COUNT         2099 a         2014 c         1805 g         1972 t
ORIGIN      
        1 tttcaattac atcctgcctg attattcaac tctattttct ctattatctg tattaataaa
       61 tgacaatgag tgtcaaacag acattattca tttcaacatg taactttggg caaaatcaag
      121 atacgcgcag aagaataatt tccgaatgaa agtagaaagt gttttattta tagatgggga
      181 gagtcttcga tagtagtgag gttgacagat atccgctgcc tgatgagcaa ttatgcccaa
      241 tgaatctacc tcattgggca taatttggaa ctcatcagat agcttttgcc atcttcagat
      301 tacacgggct catctgccag ttgaatgtgc cgtttccgct ttcatcaagc gttacgctgg
      361 caatggccga ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcaaccagat
      421 acccgactaa cggtaagtga gagatcacca gtaccgaagc aacaccttca ttggtcaacg
      481 cctgcaaata ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt
      541 ctgcactgga aggcaggttc agacaatccc ctacttcttc cagtgtttgc tcggctcgca
      601 ggaacgggct caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca
      661 tcaggcgaga ttcgtcacaa ccattagtgg tcagaggacg aacggaatca ctggcggcat
      721 cgagggctgc gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac
      781 cagattcgcc cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg
      841 ccttatccat tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat
      901 atgcgtttat attttgacca acactccgcc attcagcgcg gattcatata gctttgacct
      961 tcttattgca ggtcagttgc agttgttttc caaaaacttt ccccacgcgc gcccatctcg
     1021 accaaacgct cgcaaggggt aaaacgggaa ccatactgcg tggcaagtcg ttgcattatt
     1081 gcaaccactt cgcccgcgcc gagagaatcg atatagcgga acggtccacc gagaaatggc
     1141 ggaaaaccaa tgccaaatac cgcaccaata tccccgtcac gcacgctacg gataacctgc
     1201 tcatcaacac aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc
     1261 ggtgcggaga gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc gggatcgacc
     1321 tgttttttgc ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg
     1381 cctttgcgat cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct
     1441 ccataagcgg cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc
     1501 aaaagttgga ttggacctac cggaaaacca aatttcacta gcgcggcatc aatgtgctct
     1561 acccgttcac cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga
     1621 ttgacgtaaa acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc
     1681 agttttaccg tagtggcgat ggtttgcgcc gatgtacccg catgaggaat aatctctacc
     1741 agcggcattt tttccaccgg actgaagaaa tgcaggccaa taacttgctc aggtcgcgtg
     1801 gcgtgagctg cgatatcacc aatcggtaaa gatgacgtat tcgaagcaaa aatggtatga
     1861 gcggcgcaat tttgctccac ttccgccacc atctgttgtt ttaattcgag attttcaaac
     1921 accgcttcaa taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt
     1981 aatgccagct gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc
     2041 agctgatccc aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt
     2101 ctgaccggaa gccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca
     2161 ccacctaaaa ttcccacgct gtttaatggc gcaggcggtg catcactacc gggatctttc
     2221 ttcacttccg tactggcaaa aaagatacta cgcaaagctt gcgattgtgg cgtcatcgcc
     2281 agttcgccaa acgcccgagc ttcggcgtca taaccgctgc tggtgccctg cgctaatccc
     2341 gtttcaacaa cctccaggat gcgttctgtc gccggataat tgccttgagt tttgtgttct
     2401 gttttcttgc cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc
     2461 tcgcgtacag gtagagggcg ggaagatggg cgatccttct tcgccagctc aacagcgact
     2521 tccagcaaaa tggagtgcgg aacaacgtca tccaccagcc ccagctttaa tgcctgtttc
     2581 gcccgaagtt gttttccggt gaggatcatc tctaatgctg tgctgacgcc tatcagacgc
     2641 ggtaaacgct gggtgccgcc tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg
     2701 agcaccgttt taggatcgtc agtacaaacg cgaccgtggc acgccagcgc caactccagc
     2761 ccaccaccca ggcaagcgcc atgaatagcc gcgataaccg gaatgggcaa agcatgaatc
     2821 tccgccatca actgttggcc ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag
     2881 ttgccgatca tgttgatgtc tgcgccagca atgaagttgt ccggtttagc ggagacaaac
     2941 accacgcctc gcaactcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag
     3001 gcaaactccg ccttcagggt attcattttc tcacccggta cgtcgatggt gataacggca
     3061 atgttgtcca gacgaacatt aagggtaaac gctgatgtca tttccattat tccgcctcca
     3121 gaaccattgc cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc
     3181 cgcgacggcg aagttcatgc aatgtctggg taatcatccg tgcgccggtc gccgcgaagg
     3241 gatgtccgta agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag
     3301 tggcatgtgc acgccccagt acttcacgag caaaacgttc actacccagc aactgaatat
     3361 tcgccagtgt ctgagctgca aaggcttcgt gcatatcgat caatgtcaga tcgctcattg
     3421 tcaaaccggc acgttccagc gccagcggtg ttgaccaggc cggaccgagc aacatgtcct
     3481 gccagacatc aatcgcagta aatgcgtagc tgcgcagata ccccagcggc accagcccta
     3541 attctttcgc ccgggattca gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg
     3601 tactgtttgc cgccgttacc gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt
     3661 aatcggcaag cgaggaatta ccgcgaatat tgttgtcttc gacaagcggt tgtttataag
     3721 gagggataaa ggcagtcatc acctcttctt tgagttttcc gtctgaccat gcctgagcgg
     3781 cacgctgatg cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt
     3841 tcgccatttg ttcggcggtg tcgcccatcc gcaagccagt agaatattct gctaccgcag
     3901 gcggtacggg cattaagtcg cgcaaacgca ggcgagagaa gagtttcagt cgctggctca
     3961 tggttcgagc tttgttgaca tcaaccagca cgcgcgccag ttttttactg acgccaattg
     4021 gcaatacgga agaggaatct gccccaccgg caatccccgc tcggatagtt cccgccatca
     4081 ggctttctgc gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt
     4141 aagcatcggt atgcacattc attccggtac cgagaacaat ttcacgcgca atgttggggg
     4201 cttcaggcat ttgtacgacc tgaccaaaga ccagctgttc aatcacttca gcggggatct
     4261 cgctgcgtgc cagcagttcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat
     4321 gaaaagccgt cgcctggcgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat
     4381 cgccctggcg ggtaaccagc ggtaaaacct gacccataac actcccctgt aaaaaaataa
     4441 ataaagtggt ctgacctgat catagtctta accatttttt tacatttagc caagcggaga
     4501 aaaaggaaag tgggagctat gacacagtga agacaggaga aagaaaagaa aacgcccctg
     4561 ccagcaactg acaggggcgt acgtacccga gaggaattaa cgcagaccca gctggaagat
     4621 cagcatttca gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc
     4681 ttcagtgaaa gtcggggtga ttttacacgg ttcggattcc acgctacggg ctttttcagt
     4741 cagcgccgcc agcgtttgtt ctgcttctgc gcggcttgca aacacgcggc tgtaagacgc
     4801 ggtgcagtcg gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc
     4861 atcagcacta catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata
     4921 aacgttgtgg atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat
     4981 cggataagtg accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc
     5041 gagtgcgcaa cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa
     5101 agatggaaac atacttgcaa cattccagct ggtccgacct atactctcgc cactggtctg
     5161 atttctaaga tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt
     5221 ttgtataaaa ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt
     5281 ctgctctcgc agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt
     5341 taaacgaatt ttcttcctct ggcctgggcc gggcttattc aggggaaggc gcaattgccg
     5401 atgatgcagg taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat
     5461 tttctgcggg tgcggtttat attgacccgg atgtgaatat cagcggaacg tctccgtctg
     5521 gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg
     5581 ttgcaccgat taacgaccaa tttggttggg gcgcttctat tacctctaac tatggtctgg
     5641 caacagagtt taatgatact tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa
     5701 ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg
     5761 gtttcaacgc cgtctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt
     5821 tggttgctgg tcagattatg caatctcctg ccgggcaaac tcctcaaggg caagcattgg
     5881 cagctaccgc caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt
     5941 ggggctttgg ctggaacgcc ggaatcctgt atgaactgga taaaaataac cgctatgcac
     6001 tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc
     6061 gtgcgtttaa taactacggt ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg
     6121 gttatctgac gctgaacctg cctgaaatgt gggaagtgtc aggttataac cgtgttgatc
     6181 cacagtgggc gattcactat agcttggctt acaccagctg gagtcagttc cagcagctga
     6241 aagcgacctc aaccagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt
     6301 accgcatcgc gttgggtacc acttattact acgatgataa ctggaccttc cgtaccggta
     6361 tcgcctttga tgacagccca gttccggctc agaatcgttc tatctccatt ccggaccagg
     6421 accgtttctg gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgacg
     6481 ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg
     6541 agtctgaagg taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacgcg
     6601 ttcgcctgga taaagtcacc tgcatagcag gtgactttaa ctcccccact tcaccgaaag
     6661 tagtgtcctc attgcttacc tcctgagttt tgcaaacagc ctgatggcag cttgcccttt
     6721 caaatcaata agcggtatcc acagacaaat ctgaagggaa aggcatattt tcaggcgttc
     6781 tgctcgtcct tctcaaagag tttacttttc tgaatttcca ggatactcac ccccctggct
     6841 attgtgcgct catacactca aattaaagat aggttctaaa taaatgagcg ttttttgata
     6901 gtctatttca ttaggtaata tatatttgta acaaatcaat caaaatggaa taaaatcatg
     6961 ctaccatcta tttcaatcaa caataccagc gcaacttacc cagaatccat taatgaaaat
     7021 aacaatgatg aagttaatgg attagtacaa gagctcaaaa acctttttaa tggtaaggaa
     7081 ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac
     7141 gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga tattggctcc
     7201 aatgatacag accatattac tattggtatc gacaaccaag aaccaataga attacctgcg
     7261 aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact
     7321 catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagat
     7381 cctggtttta tcttcgataa gatatcacac aaactcagac atacggtaac ggagtttgat
     7441 gagagcggga atagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc
     7501 gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc tctcggttac
     7561 tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga tagtctcacc
     7621 caatattgta gtgaaaaagc caggaatgct tcagcgtggt ttgaaagcag taaagctatc
     7681 atggcagaat catttgcaac aggttccgat catcaggttg taaacgagct caacggggaa
     7741 agactgagag aacctaacga agtttttaaa cgtttaggtc gagcaataag atataatttt
     7801 caagtggacg atgcaaaatt tagacgcgac aatgtaaaag aaataatttc taatttattc
     7861 gctaacaaag ttgacgttga ccatcctgaa
//
LOCUS       DAZFDP010000195         7861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFDP010000195 DAZFDP010000000
VERSION     DAZFDP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..573
                     /locus_tag="ACHJ1G_004505"
     CDS             49..573
                     /locus_tag="ACHJ1G_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO4766240.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            657..1343
                     /gene="lpfB"
                     /locus_tag="ACHJ1G_004506"
     CDS             657..1343
                     /gene="lpfB"
                     /locus_tag="ACHJ1G_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO4766241.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1367..3898
                     /gene="lpfC"
                     /locus_tag="ACHJ1G_004507"
     CDS             1367..3898
                     /gene="lpfC"
                     /locus_tag="ACHJ1G_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO4766242.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3909..4964
                     /gene="lpfD"
                     /locus_tag="ACHJ1G_004508"
     CDS             3909..4964
                     /gene="lpfD"
                     /locus_tag="ACHJ1G_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO4766243.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4970..5494
                     /gene="lpfE"
                     /locus_tag="ACHJ1G_004509"
     CDS             4970..5494
                     /gene="lpfE"
                     /locus_tag="ACHJ1G_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4766244.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5747..7438
                     /gene="eptB"
                     /locus_tag="ACHJ1G_004510"
     CDS             5747..7438
                     /gene="eptB"
                     /locus_tag="ACHJ1G_004510"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4766245.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7530..7606
                     /locus_tag="ACHJ1G_004511"
     tRNA            7530..7606
                     /locus_tag="ACHJ1G_004511"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7564..7566,aa:Pro,seq:cgg)
     gene            7641..7774
                     /locus_tag="ACHJ1G_004512"
     ncRNA           7641..7774
                     /ncRNA_class="other"
                     /locus_tag="ACHJ1G_004512"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2048 a         1808 c         1991 g         2014 t
ORIGIN      
        1 ttgtatgaac aacatcaagc atatttatct atataaatgg agttataaat gaaaaaggtt
       61 ctgtttgcct tatctgcgct tgcgctgacc tctacttccg tattcgcagc tgatgcgggc
      121 gacggtactg ttaaattcac cggtgagatt gttgacgccg cttgtgttgt gtctccagac
      181 actcaaaaac aagaagttgt tctgggtcag gtgaacaagt ctgtatttac tgctatgggt
      241 gataaatcca cagcaacgcc gttcaaaatc aaactggaaa actgcgatat ctccactttc
      301 aaaaatgtgg aaatcagctt taacggcgtt ggcgatgcag acaacagcaa actggtttct
      361 gtaagcactg aaccaggtgc cgcaactggc gtaggtatcg gtatttatga taacaccaat
      421 accctggttg acctgaatac tggtaaatct gctactgtcc tgaaagaagg tcagaccgtt
      481 ctgtatttca ccgctaacta tgtctctacc aaagatgcag taaccacggg ttacggtaac
      541 gctgaagtcg acttcaacct gacttacaac taatcgatcc tgacggaacg atcatctctc
      601 aatggcaaca gagactgtgt tgccattatt cacaccaatg actcagggac cgaatcatga
      661 aacgtattag ctttgctgtg ctggcacttt taatgaccgc acaacagtgt tttgctggtg
      721 gcgtagcctt aagcacgacg cgtgttattt atgatggcgg caagaaagaa gcgtcgctga
      781 ccgttgaaaa ccataataaa aatgaagagt ttttgatcca gtcatggatt gatgatgtga
      841 atggcaataa aaaaacaccg tttattatta caccgccatt attcaaactt gatcctgata
      901 aaaataacgt cttacgtatc gtcaatattg aagatacctt acctaaagat cgtgaatccg
      961 tttactggat taatgtaaag gccattcctg cgaaaagtgc tgaaaatgaa aataaaaacg
     1021 tcttacagat agccgttcgc acccgtttga agttgttcta tcgtccaaaa ggtttgccgg
     1081 gaagcaacct cgaggccatg aaacagttac gtttccagcg tcaaaataac caaattgtgg
     1141 ttaataacga ctccgcttat aacctgacct ttaatcagtt ttccattaat ggacaaaaga
     1201 ttgaacgggc gggaatggtt ctcgcgaaag gaaagttaaa cattaacctt ccagccggta
     1261 caggcaacgc gcacgaagtg aaatacagca ttattaatga tttcggtgtt gctggcgaaa
     1321 tgctgacgaa gccgattaac tgatcctttt acaaggcgct tttactatgc attggatgca
     1381 tttacctctc tatcactaca gagcgcattt ttccttctct ctgctggcat taacgattgc
     1441 cagctcgtta cctgcgtatg gcggaaaatt taaccctaaa tttctcgaaa acgttcaggg
     1501 tatcgatcaa cacatcgact tgtcggtata cgactcacct gtcggccagc aaatccccgg
     1561 taaataccgg gtttcggtgt ttgtaaacga agaaaaaatg gcttcccgga cgctggattt
     1621 tagtaccgcc agcgaagcga aacgcaaagc cagcggtgag tcgcttatgc cttgccttag
     1681 ccgggtgcaa ctggaagaaa tgggcgtgcg cgttgacagc tttccggcgc tgaagatgtc
     1741 accaccagaa gcctgcgtag cgtttgatga aatcattccc caggccacca gccgctttga
     1801 ttttaatact cagacgctgc atctttcctt tccacaggcg gcaatgatga tgaccgctcg
     1861 cggcacggtt gatccttctc gctgggacga aggaatacct gcgctgttgc tggattacag
     1921 ctttagtggc agcaatggac gcaatgaagg tacaggttca tcttcagatt cgaccagtga
     1981 cagttattac ctgaacctgc gcagcggttt gaacgttggt ccgtggcgtt tacgtaataa
     2041 cagtatctgg aaccgtaccg atggcaaaaa ccaatgggat aacgttggta catcgttaaa
     2101 ccgggcaatt attccactta aatcacagat aactctgggt gataccgcca cgcctggcga
     2161 gatttttgat agcgtacaaa tgcgtggggc gttactggca tccgacgatg aaatgttgcc
     2221 ggacagccag cgtggctttg ctccggtagt gcgcggcatc gccaaaagca acgctgaagt
     2281 gagtatcgaa cagaacggct atgtcattta tcgcactttt gttcagccgg gtgcatttga
     2341 gattaacgat ctctacgcaa cctccggcag cggcgacctg acggtgatca ttaaagaagc
     2401 cgacggaagt gaacaacgtt ttatccagcc gttttcggcg gtggcgattt tccagcgtga
     2461 agggtatctg aaatacagtc tggctgccgg tgaatatcgt gctggtaatt acgacagcga
     2521 taaaccacat ttcgggcagt ttaccgccat gtacggcttg ccgtggggta tgaccgccta
     2581 cggtggtgca ttgttgtcgg cagattacaa cgctttagct ctgggcctgg ggaaaaactt
     2641 cggtaccatt ggcgcagtat cggtggatgt cactcaggcg aaaagtcagt tacgcaacaa
     2701 tgaaaaagag gaaggccagt cttatcgctt cctctattcg aaaagttttg agggtggcac
     2761 tgacctgcgt ctgttgggct acaaatactc aaccagcggc tactacacct tccaggaagc
     2821 aactgatgtt cgcagtgacg ccgacagtga ttaccgccgt tatcacaagc gcagccagat
     2881 acagggcaat attacccagc aactgggaga ctacggctcg gtttatttca atatgacgca
     2941 gcaggattac tggaacgttg acggtaagga aaactcctta agcgccggtt atcacggtca
     3001 cattggtcgg gtgaattaca gcgtggccta tacctggacg agaagtccgg aatgggaaga
     3061 agacgatcgc ttgtggtcat tcagtgtttc catccctctg gggggcgcgt ggagcagtta
     3121 tcgcatgact accgaccaga atggcaagac gtcgcaacag gcctctgtta gcggcacgtt
     3181 actggaagat cgtaacctga gctataacgt tcagcagggt tacaccagta atggcgtagg
     3241 ctacagtggc agcgtcaaca tggggtatat gggtggctct ggtaacattg acgtaggcta
     3301 taactattct aaagataacc aacaggttaa ctatggcgtg cgcggcggtg tcatcgttca
     3361 cagtgaaggt ataacgcttt cgcagccttt aggcgaaagt ctggcgattg tttctgcacc
     3421 gggcgcgcgt ggtggacatg tggttaacag cagcggcgta gaagttgact ggatgggcaa
     3481 cgccgtggtg ccttatctca caccatatcg tgaaaccatt gtggaattgc gtagcgacac
     3541 gttggggcaa aacgttgaac tacaggaagc gttccagaaa gtcgtgccta ctcgtggtgc
     3601 agttgtgcgt tcccgctttg atacccgcgt cggttaccgt gtgctgatga gcttaaaaca
     3661 ggcgaatggc aacgcagtac cgtttggtgc aaccgcagcg ttaattgatg agagcaaacc
     3721 tgccagcagc attgtgggtg aagaaggaca gctttatatc agcgggatgc cagaagaggg
     3781 cgaattgcag gtgagctggg ggaacgagca ggcgcaacgt tgccgtgtgc cattccgctt
     3841 gccagagaac aaggacaatg cggctatcgt gatggttaat gcggtatgcg agaaataagg
     3901 atatttagat gaaaattaaa aatttgttgg caggtatggt gctgttaggg acgagcactt
     3961 ttgctggaaa tatatgggct gcggattggg gaccatgtca gacatcaaat ggggtggccc
     4021 atgagtatag ttttgatttc gtacagacta tccaggtacc ttcagaaaat aaggctggga
     4081 aaatattaac tcagcccttt gctttaggta caaaatattc cgcttattgt gaatgcccgg
     4141 atcccattcc agataatgga gttgtgactt attttaaagg tgtaacgctt ctgcctgagc
     4201 ctggaactgt agatgggtac tataaattca ataattacat tgatattctc accaaaatat
     4261 atgtctacac acaattagat atcccggtgc cttttaccga tcgaagcaat ggcacggcac
     4321 agaaagaatg tacgccctat actgctaaca attgggggac aggtggtaaa ggaagtatca
     4381 gtatttatat ttcacatcct tttgttgggc aaatgattat tcctaaaaca cgagttgcgt
     4441 ccctctttgg gacaaaaaag aaaggtgttt acaatgatag tcagcccatg gccaatgtca
     4501 gtatttccgg gagtattaca gttactcaag gctgtgaact tgccgctggc accgtactgg
     4561 atattccatt cggagaatat caggcccatg attttaaagg gcgtgccgga caaccaccac
     4621 aaaatgttca gaaggtacaa aaagaattaa gctttgactg caataatatt tcagatggcg
     4681 tcaaaattta tctcagtatt gatgcaacac caaatactgc ctatcccagc gcaattgatt
     4741 taggcaacgc cgatgttggt gcagtcattg aggacggtaa aggtaatatt cttaagccga
     4801 atgacagtaa tagcttatta gaaatgaacc caggctcgct gtatgaagat gtcaaacgca
     4861 aagcgacaac cacaattacc gcttatcccg ttagcactac cggaaaatta cccgctgccg
     4921 gggattatag cggtattgct accatacatg tcgaactgga gtaacagtca tgaagcgaac
     4981 aatacttgcg ttatgcctgc tgcctctttc cttttcgtca ttcgcggcaa ccacggattt
     5041 agggccgaaa ggaacattga aattcaccct taaaatcagc cagggtgctt gcgaactggt
     5101 gaaagacagc gttgaggttg acatgggcac cgcggttctc aaaaagccgg taagaatcgg
     5161 aacggaaatt aatcccactc ctttttccat cggcctgaaa aactgtagcg aagtggtcag
     5221 agcctacgtg actatggacg gtacacccaa tgcagacgac tcaaatcttt ttgcgctgga
     5281 tgcgggtggc gctacaggcg ttgggctgaa aatcaaaacc gctgctggag tacaacagtt
     5341 cccggaaaat acaacgccga caccggttga gtttgcaatt aacggtgatg gcgcacatca
     5401 actgaactat atcgccagct acgtgcctat tcgcgccgat gcaactattg gtcgcgcaga
     5461 tgcaacggtc gactttactg tacagtacga ataatttcac atcttaaggg ccagttaact
     5521 ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca tttttgtaaa
     5581 tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt ctacttattc
     5641 gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg ggcctgaaaa
     5701 gccgctaagc agggtgttat cacctgtttg tccagggttt gtttgcatga gatacatcaa
     5761 atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc tttttatgaa
     5821 tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg tctggaaagg
     5881 cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt tactacgtct
     5941 tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg tgctcttttc
     6001 cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg gcatcatcgc
     6061 ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga actttattct
     6121 ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc gctgtcgcta
     6181 caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt
     6241 actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc agaagaaagt
     6301 ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga actcttatct
     6361 gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg atgaatcttc
     6421 cgataataat tcattgctta atccggcgaa gaaattcact tatcaggcac cgcaaaacgt
     6481 tgatgacact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc atatgggcat
     6541 tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc tggcggcgtt
     6601 ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt ttgtacgtca
     6661 ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt tcgcggttct
     6721 gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt ggttctacag
     6781 caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc cacgtaatcg
     6841 tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc tagggcgcaa
     6901 cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta actacaccca
     6961 gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg atagcggctg
     7021 taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg atcactttat
     7081 ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg cagctgacca
     7141 cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc
     7201 ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg aaaatccggc
     7261 caatgctcag gcatttgcgc agctgaaaaa agaagccgac atgaaagtgc cacgccgtca
     7321 tgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg atggtggaat
     7381 taacgaaaac aacaactggt gtcacatccc gcagacaaaa gtggcagcgg ctaactaagc
     7441 ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt gacgagggcg
     7501 tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcctggt agcgcacttc
     7561 gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt cgaaaagcct
     7621 gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct ccgggggcgg
     7681 ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg cagggcgagc
     7741 cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc tcaacgagca
     7801 ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcgggg aggttcgact
     7861 c
//
LOCUS       DAZFDP010000196         7743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFDP010000196 DAZFDP010000000
VERSION     DAZFDP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            321..863
                     /gene="ecpR"
                     /locus_tag="ACHJ1G_004513"
                     /gene_synonym="matA"
     CDS             321..863
                     /gene="ecpR"
                     /locus_tag="ACHJ1G_004513"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO4766246.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            937..1524
                     /gene="ecpA"
                     /locus_tag="ACHJ1G_004514"
                     /gene_synonym="matB"
     CDS             937..1524
                     /gene="ecpA"
                     /locus_tag="ACHJ1G_004514"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO4766247.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1582..2250
                     /gene="ecpB"
                     /locus_tag="ACHJ1G_004515"
                     /gene_synonym="yagY"
     CDS             1582..2250
                     /gene="ecpB"
                     /locus_tag="ACHJ1G_004515"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO4766248.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2276..4801
                     /gene="ecpC"
                     /locus_tag="ACHJ1G_004516"
                     /gene_synonym="yagX"
     CDS             2276..4801
                     /gene="ecpC"
                     /locus_tag="ACHJ1G_004516"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO4766249.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4791..6434
                     /gene="ecpD"
                     /locus_tag="ACHJ1G_004517"
     CDS             4791..6434
                     /gene="ecpD"
                     /locus_tag="ACHJ1G_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO4766250.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6403..7113
                     /gene="ecpE"
                     /locus_tag="ACHJ1G_004518"
     CDS             6403..7113
                     /gene="ecpE"
                     /locus_tag="ACHJ1G_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO4766251.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7426..>7743
                     /gene="ykgJ"
                     /locus_tag="ACHJ1G_004519"
     CDS             7426..>7743
                     /gene="ykgJ"
                     /locus_tag="ACHJ1G_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4766252.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGL"
BASE COUNT         2021 a         1971 c         1988 g         1763 t
ORIGIN      
        1 tatattgcca ataaccttat gaaaccaaat gtctttttct tcttatcaaa aaagcaatat
       61 tttcagtttt tctaaatatt gacttaacca ttgaattcct tttccgttca catattgaca
      121 ctcatcggga aaaaaacata aatttaagcc caatcgaaaa taattaaact taatctcgtt
      181 taacctttat tgatatgtac tacgtatctt atttacttcc ggtttactaa cgaaactgaa
      241 tgcacctgta aaaattacag gtttggaaag tagtgacatg gcaaagtgat tacagcaggg
      301 actatgaggt taaaaaccat atggaatgtc aaaaccgttc tgataaatac atctggtctc
      361 cccatgacgc ctacttctat aaaggactat ctgaactgat tgtggatatc gacagattaa
      421 tttatctatc gttggagaaa attagaaaag atttcgtgtt tatcaatctc aatacggatt
      481 ctttatctga atttataaac cgtgataatg aatggttatc cgcggtaaag gggaaacagg
      541 tcgtattgat tgcggccaga aagtcagaag ccttagcaaa ttattggtat tacaacagca
      601 atattagggg cgtggtatac gcaggactga gtcgtgatat tagaaaagaa ctggcctatg
      661 tgattaatgg caggttcctg agaaaagata ttaagaaaga taaaatcacg gaccgggaaa
      721 tggaaattat ccgcatgacg gcccagggaa tgcaacctaa atcgattgcc agaattgaaa
      781 attgtagtgt gaagacagtg tatacccatc ggcgtaatgc tgaggccaag ctgtactcaa
      841 aaatatataa gttggttcag taaactccag gctagttagt tttaaaaatg actcactggg
      901 acatcacgtc ctcaattcaa ctcgggaaga aatgcaatga aaaaaaaggt tctggcaata
      961 gctctggtaa cggtgtttac cggtataggt gtggcgcagg ctgctgacgt aacagctcag
     1021 gctgtagcga cctggtcagc aacagccaaa aaagacacca ccagtaagct ggttgtgacg
     1081 ccactcggta gcctagcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa
     1141 ggtctatttg acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca
     1201 cgtcttatca ccaacacatt aacccagttg gatacctcag gttccacact gaatgtgggc
     1261 gtggattata acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc
     1321 aacggcgtac tgggcggcaa ccttagcccg ctggcaaacg gttacaatgc cagcaatcgt
     1381 accaccgcac aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc
     1441 gcagtaaccg attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag
     1501 ttcgacgcga cctggaccag ttaatctctc tgatgtacca gcaggggtag cccccctgct
     1561 ttattcctgg acggaattgt tatgaaaaag caccttctgc ctctcgctct gctgttttcc
     1621 ggaatatctc cggcccaggc gctggatgtc ggcgatatat catcgtttat gaacagtgac
     1681 agcagcacgc tgagcaaaac gatcaaaaac agtaccgaca gtggtcgcct tatcaatatc
     1741 cgtctcgaac ggctctcttc accgcttgac gacgggcagg ttatctcaat ggacaagccg
     1801 gatgagttgc tactcactcc cgccagcttg ctgctacccg cccaagccag cgaagtgatc
     1861 cgcttcttct ataagggacc cgcagatgaa aaagagcgct actaccgcat tgtctggttt
     1921 gatcaggccc tcagtgatgc gcagcgcgat aatgccaacc gcagcgctgt ggccactgct
     1981 tccgcccgca tcggcaccat tctggtcgtc gcccctcgtc aggcgaacta ccactttcag
     2041 tacgccaacg gctccctgac aaatacagga aatgcgacgc tgcggatcct cgcttacggc
     2101 ccctgcctga aagccgccaa tggtaaggag tgtaaagaga attactacct gatgccaggc
     2161 aagtcgcgtc gttttacccg cgtggacacg gcggataaca aaggacgggt tgcactttgg
     2221 cagggtgata agttcattcc cgtgaaatag atagctgtgc agatggataa cgacaatgcc
     2281 tttacgacgg ttctccccag gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt
     2341 cgttcaaccc gatgccagcg ctgctgacat aagtgcgcag caaataggtg gggtgattat
     2401 tccgcaggcc ttcagtcagg cgcttcagga cggcatgagc gtcccgctct atattcatct
     2461 cgccggtagc cagggtcgcc aggacgatca gcgaatcggc agcgctttta tctggctgga
     2521 tgatggacag ctacgcatcc ggaaaataca gctggaagag agtgaagata acgccagtgt
     2581 cagcgaacaa actcgacagc agctgatggc tctggcgaac gccccgttca atgaggccct
     2641 taccatcccc ctgactgaca acgcgcagct ggatctcagc ttgcgccaac tgctgctgca
     2701 gctggtggtc aagcgcgaag cgctgggcac cgtactacgc tcacgtagcg aagacatcgg
     2761 gcagtccagt gttaacaccc tcagcagtaa tctgagctat aacttgggcg tctataacaa
     2821 ccagttgcgt aacggcggga gcaacacatc cagctatctg tcgctgaata acgttactgc
     2881 gctgcgcgaa catcatgtgg tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca
     2941 ggacagtgaa ttatataaag cgatgtatga acgcgatttt gccggtcacc gatttgccgg
     3001 tggaatgctc gacacctgga acttgcagtc cttagggccg atgaccgcca tttcagcagg
     3061 gaagatttac ggcctttcct ggggaaacca ggccagctcc accatcttcg acagcagcca
     3121 gtcagccacg ccagtgatcg cctttttacc ggcggcgggc gaagtacatc tcacccgtga
     3181 tgggcggcta ctaagcgttc agaacttcac tatgggcaat catgaagtgg atacccgggg
     3241 tctaccgtac gggatttacg atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag
     3301 caaacgcccc cagcgggtca ataagctgtt tagccggggg cgcggcgtcg gtgcaccact
     3361 ggcgtggcag gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa
     3421 gacgcgacca gctaaagaga gttggctagc aggtgcctcg acctccggct cattgagtac
     3481 gcttagctgg gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct
     3541 gacgctgccg cttggggggg cgatcaacgt taacctgcaa aatatgctgg ccagtgacag
     3601 ctcatggagc agcatcggca gcatcagcgc cactctaccg ggaggcttta gttcgctgtg
     3661 ggttaaccag gaaaaaaccc gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg
     3721 tgcaatcggc ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat
     3781 cagctacaat gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa
     3841 tgtctacagc ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa
     3901 cggcgacagc aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg
     3961 caactggttt agcgcaggga tgactcatca aaacggctac accatggcaa acctgtcagc
     4021 acgcaaacag tttgatgaag gaaccattcg cactgttggt gccaatctgt cacgtgccat
     4081 ctccggcgat accggtgatg acaaaaccct cagcggtggg gcgtatgcac agttcgacgc
     4141 tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg gacggctaca tcaataccaa
     4201 cttgaccgcc aatggcagcg tcggctggca gggtaaaaac atcgctgcca gcgggcggac
     4261 tgatggcaac gctggggtga tattcaacac cgggctggag gacgacggtc agatcagcgc
     4321 caaaatcaac gggcggattt tcccgcttaa cggcaagcgt aactatctcc cgctctctcc
     4381 ctatggaaga tatgaggtgg agttacagaa cagcaaaaac tcactcgaca gctacgatat
     4441 cgtcagcggc cgcaaaagcc atctgactct ctatccaggc aatgtcgctg tcattgagcc
     4501 agaggtgaag cagatggtta ccgtctccgg tcgtatccgt gcggaagacg gcacactgct
     4561 ggctaacgca cggattaaca accatatcgg ccgaacccga accgatgaaa acggcgagtt
     4621 tgtcatggac gtggataaga aatatcccac tatcgatttt cgctacagtg gcaataaaac
     4681 ctgcgaagtg gcactggaac tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt
     4741 ctgcagcggc ctctcatcgt gggcggaggt gacgcagaca ggagaagaga atgagagtta
     4801 acctactaat aacgatgata attttcgcgc taatctggcc agtaactgag ctcagagcgg
     4861 cagtgagcaa aacaacctgg gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca
     4921 actcagacga caactttttc gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg
     4981 ccaaccgctg gaccggttta aaatacactg gttcaggaac catctatcag caaagcctcg
     5041 gctacattga taacggttac aacaccggcc tttataccaa ctggaagttt gatatgtggc
     5101 tggaaaattc accagtttca tctcctttaa ctggcttgcg ctgcatcaac tggtacgctg
     5161 ggtgtaatat gaccaccagt cttatcctgc cgcaaaccac cgacgccagt ggattttatg
     5221 gcgcgacggt gaccagcggc ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt
     5281 accagtatct gcaacaaatg cccgtcggca gcagctttac aatgaccatc aatgcctgcc
     5341 agacctctgt gaactatgac gccagcagcg gcgcacgctg taaggatcag gcctccggca
     5401 actggtatgt tcgcaacgtc acccatacga aagcagcaaa tctgcggtta ataaataccc
     5461 actcgctggc ggaggtattt atcaacagcg acggagtacc gactctgggc gaagggaacg
     5521 ccgactgccg gacacagacc atcggcagcc tttcaggatt aagttgtaag atggtcaact
     5581 ataccctgca aacaaacgga ctcagcaaca cctcaatcca tatattcccg gcgatcgcca
     5641 actcgtcgtt agcctcggcc gtcggggcgt acgatatgca gttcagtctg aatggcagtt
     5701 catggaaacc ggtgagcaat accgcctatt actacacctt caacgagatg aagagcgcag
     5761 actcgatcta tgttttcttc tcgagcaact tctttaagca gatggtgaac ctcgggatca
     5821 gcgatatcaa caccaaagat ctattcaact ttcgctttca gaacaccaca tcaccggagt
     5881 ctggctggta tgaattttct acctccaaca cgctgattat caaaccccgt gatttcagca
     5941 tcagtattat ctccgatgaa tatactcaga caccgtcgcg ggagggatat gttggcagcg
     6001 gcgagtcggc actcgatttc ggctatatcg taaccaccag cggtaaaaca gctgccgacg
     6061 aagtgctgat caaggtgacc ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct
     6121 tcagctccga tgacggtaag gcgaaagtac cgttcccggc gacactttcc tttattaccc
     6181 gcaacggagc tacaaaaacc tacgatgccg ggtgcgatga tagctggcgg gatatgaccg
     6241 atgcgctgtg gttgaccaca ccgtggactg atatctctgg cgaagtgggg cagatggata
     6301 agaccacagt caaattttcg attccaatgg ataacgccat ttcgctgcgt acggtagatg
     6361 ataacggctg gtttggcgaa gtcagcgctt caggagagat tcatgttcag gcgacgtggc
     6421 gtaacattaa ctaaggccct gctgacagcg gtctgtatgc tggcggcacc tttgacacag
     6481 gcgatttcgg tcggcaatct gacattttcg ctgccgtccg agactgactt tgtcagcaaa
     6541 cgtgtagtga ataacaacaa aagcgcacgg atataccgta ttgccatcag tgctattgat
     6601 agcccgggca gcagtgaatt gcgcacccga ccggtggatg gtgaactgct ttttgccccc
     6661 cgccaactgg cgttgcaggc tggtgagagc gagtatttta aattttacta tcatggtcca
     6721 cgggataacc gcgagcgcta ctaccgggtc tcatttcgcg aagtccccac tcgtaaccag
     6781 acaagacgta gcccaaccgg cggcgaggtc agcacggagc cggtggtggt gatggatacc
     6841 attctggtag tacgaccacg tcaggttcag tttaaatggt ccttcgacaa ggtgacagga
     6901 acggtaagta acactggcaa cacatggttt aagctactga ttaagccagg atgtgattcg
     6961 accgaagagg aaggcgatgc ctggtatcta cgtccgggag acgtggttca tcagcctgag
     7021 ttacgtcagc cggggaatca ttatctggtc tataacgaca aattcattaa gattagcgat
     7081 tcctgtccgg ctaagccccc ttcggcggac taagacttat ccatcggtcg agggaagaat
     7141 tccacctcag agctccaaaa atcgtttaaa tgatgtggaa gcgatcgtca atgtcacctg
     7201 taacgatggg ctttctcata tgcacaccat agtgttcagg agacacggaa aggtatcagg
     7261 cagcctgcta atgagtggtt taatcacgaa ttagtacgta aaatcggtaa cggctggaaa
     7321 tcattcaata ctcacactat cgaaagttcc ccagccaacc gcggtacgtt cttacatacg
     7381 atgtaccgct gttctcttta cgatttttag ctgtactggt gaattatgag caatctgaat
     7441 ccatgcatga cgtgtggtgc ctgttgtgca tttttccgcg tctcttttta ctgggccgaa
     7501 gccgacgatg ctggcggtac tattcccgcc aggctcactg aacaaatatc cccttttcac
     7561 cgatgcatga gcggtaccaa tcagaaaaac ccccgatgta ttgcccttgc aggaaccccg
     7621 ggcaaaaatg cctgctgcac gatatataaa aatcgatcgt ccacatgcag agaatttgcc
     7681 atgtctggtg agaacggagt cgtcaatgag gcttgcaatc gtgcaagggc taaatacggg
     7741 ctg
//
LOCUS       DAZFDP010000197         7721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFDP010000197 DAZFDP010000000
VERSION     DAZFDP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..275
                     /locus_tag="ACHJ1G_004520"
     CDS             <1..275
                     /locus_tag="ACHJ1G_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFO4766253.1"
                     /translation="QLGASDPEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHA
                     QRISETTERASRKADLLRGSLTSGNKKQTETGFTNPVNEQKTCRDI"
     gene            352..1311
                     /locus_tag="ACHJ1G_004521"
     CDS             352..1311
                     /locus_tag="ACHJ1G_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4766254.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLIADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYDSLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIAAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            1316..1627
                     /locus_tag="ACHJ1G_004522"
     CDS             1316..1627
                     /locus_tag="ACHJ1G_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4766255.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPASQPVPTTQITSRMAGNIRALFP
                     E"
     gene            1691..2023
                     /locus_tag="ACHJ1G_004523"
     CDS             1691..2023
                     /locus_tag="ACHJ1G_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFO4766256.1"
                     /translation="MQHIDREKAQRLIERMEALAKEENVNIQKIAECGQIVLRREKDL
                     KQLMSGETSKTWTSGEETIYCSFCNKSQYEVTKVIAGPSVCICNECVDLCNEIIRREV
                     TDKKGKNA"
     gene            2020..2358
                     /locus_tag="ACHJ1G_004524"
     CDS             2020..2358
                     /locus_tag="ACHJ1G_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766257.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQAKPSDGLVRAVRFYELVKRENPPAETGAWKDAVDWVLKEACQVVNTGIK
                     GDLAQPASKL"
     gene            complement(2850..3896)
                     /locus_tag="ACHJ1G_004525"
     CDS             complement(2850..3896)
                     /locus_tag="ACHJ1G_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766258.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(3896..5647)
                     /locus_tag="ACHJ1G_004526"
     CDS             complement(3896..5647)
                     /locus_tag="ACHJ1G_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO4766259.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            5802..6638
                     /locus_tag="ACHJ1G_004527"
     CDS             5802..6638
                     /locus_tag="ACHJ1G_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO4766260.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDSQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            6662..7714
                     /locus_tag="ACHJ1G_004528"
     CDS             6662..7714
                     /locus_tag="ACHJ1G_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO4766261.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
BASE COUNT         1968 a         2103 c         2004 g         1646 t
ORIGIN      
        1 cacagctggg ggcatcagac cctgaagccg ccgcacgcct ggcatccaca cttcagcagg
       61 accagaacac catgaaaaca cccgttaccg tgctttctga catgctgcgc gccatccgcg
      121 acgcagagca cgcacagaga atcagtgaaa ccactgaacg cgccagccgc aaagcagacc
      181 tgctgcgggg tagcctgacc agtggaaaca aaaaacagac agaaacggga ttcacaaatc
      241 ccgtaaatga gcaaaaaacg tgccgcgata tatgaagcgc gcacaaaaca ggcaaaagcg
      301 ggatttaaaa atcctgtaac cgattaatta atcaacataa ggaaaaacga catgaaaatt
      361 tgtatcgacg acggctccac caacatcaag ctggcatgga ctgagaacgg cgaacgccgc
      421 aacgccatca gcccgaacag cttcaagtcg gaatggtctg cgccgttcgg tggcacgcag
      481 cccgcgaact acatgcttga tggcgtgcgc tatggttttg atccggtcag cgatcgcttt
      541 gtccagacga ccgacacgca ataccagtac agcgatgtga atgtcattgc cattcatcac
      601 gcactggtca aatcaggcat cacgccacag gaggtggatg tggttgtcac cctgccactg
      661 agcgagtatt tcgacacaaa cgcacagccg gacatggcca acatcaaccg caaaaaagcg
      721 aacgttatgc gcccggtgga gtaccagaac ggcgaagcat tcactatccg taacgtgcgg
      781 gttatgcctg aatccattcc ggctggcttt aaggcactgg ctgacatgag tccgtttgaa
      841 tccctgctga ttgtggattt aggcggaacc acgctggatg tggcaaaggt ccagggacaa
      901 ctggcaggta tcagccaggt gttttgcgat ccacacgtag gcgtttccct gatagccgat
      961 gccgtactgt cggtgatggc cactaacggt atgcgtacca gtcaccatat cgccaatacc
     1021 attatcgaac atcgccacga tgaagcctgg ctgcgccagc acatccacaa tgacgcgcat
     1081 tacgacagcc tgatggcggt tattcgtgaa aaggaagaaa cactgaaaca acgagtgatc
     1141 cgcgcgctgg cgggtttttc gggttacggg cgggtgatgg ttgtcggtgg cggggcggag
     1201 attgcggcac ccgctatccg cgaagcctgc ggagttaatg cgactttcat cgcggacggg
     1261 gtgccacagt ttgctctggt taatgggctg tacgcaatgg acaaggagta aaccaatgac
     1321 gacaccaacc agacggataa gtttctatct gaagcccgcc gccgtcaaga acgaaggcga
     1381 agcatgcgcc tggctggaca gccttacacc agaagcccgc aaaagcggcc aacgtgtggc
     1441 ttttctggcc gggctggcac ttctgaaaat gaatccggca gaggcttacc gactggctgc
     1501 atgggctgac gatgaggcgt tatcagtgac acaaaccagg acagaacgcc ccgcgtcaca
     1561 gccagtacca accacacaga taaccagtcg gatggccgga aatatcaggg cgttatttcc
     1621 cgaataacac aacatcaggg cgcatccgcc ctgatgactt taatccggga acataaacaa
     1681 gggggacaca atgcaacaca ttgacagaga aaaagcgcag cgactgattg agcggatgga
     1741 agcgctggcg aaagaagaaa atgtcaacat ccaaaaaata gctgaatgtg gccagatagt
     1801 tcttcgtcgt gaaaaagacc tcaaacaact gatgtctggc gaaaccagca aaacatggac
     1861 ttcaggcgag gagacgattt attgcagctt ctgcaataaa tctcagtacg aagtcacaaa
     1921 ggtgattgcc ggaccgtctg tttgcatctg caatgagtgc gtggatttgt gcaatgaaat
     1981 tatcaggaga gaagtgacag acaaaaaggg aaaaaacgca tgaacagaaa acaaaaacaa
     2041 gagttgaaat actttttacg taaagaaatt gccaggcttg aagatgcaga atcacaatca
     2101 tcagaaattc cgttcggaat ggatatcaac gacgcccgta tgctccaggc ataccgcata
     2161 gctcaggctg cactacaggc aaaaccgtca gatggactgg ttagagcagt acgattctat
     2221 gaactggtaa agcgtgagaa tccgccagcc gaaaccggag catggaaaga cgctgttgac
     2281 tgggtactca aagaggcttg tcaggttgta aacactggca tcaaaggaga cctagcccag
     2341 cctgcatcaa agctatagaa atgagccaaa acgccctctt ccagggcgtt tatttcaatg
     2401 cacaatagtg cacaaatttg cacaattttt ttgaacgact ttttgccctt ccggcccgcg
     2461 tggcagttgg atccgtcaag gatccgtgcg tgcacaaaaa aacgcgcttt ttctgcgcgc
     2521 aggtgacggg ggaacagccc gcgtttcagg gggtaaatag catcccctga acgatgtcgc
     2581 agcaacacaa cagaatggct gtatttctca cgctgagcgt gaaaaagacg tgagggcttt
     2641 tgatttgatg gggtggcaga taaggccgtc aaaatcgcac tgaggcggcg agaacatgca
     2701 gttaacgcgg tgggattgcg taagagtctg accgtcgatg atggcaataa gcaggaaagc
     2761 gtcgtgaaat tatctgactg atacaggagc tggagagtcg gggcataaat tttttatgcc
     2821 ccggcgaagc agcagacaag cgaagcgcgt caggatgtgg gctgggtgtc cagcagtgca
     2881 taagggttaa agcggatcac ctcttcgcca agccagtcat tgatgtgctt catggcctcc
     2941 atgacgggca tcagctcgtt aattgcgtaa acccgcgcgg ccttctccac atcaccaaac
     3001 gcactttttt cacccggcat cgcccccatc agttgcggcg ggacacggtg cgcagccagc
     3061 acatcatcac gggatgccgc cttaacattc atgaactcat cctttgcggt gatctgctgg
     3121 aacggcaaaa tttgcacccc ctctttgccc ccgtttggcg catgaatgag cacgttttta
     3181 aacgcaccac caccacgtgc accctgtaac gtttctttca gggagtccat gctttcgcgg
     3241 tttacctgcg ctgcaccgat gtagatgatg cacccggcgt gggatccgtt gtcgtaatac
     3301 agttttctga acatgtccgc cgaatgagac aggctggccg agagtaatgc gccgagatat
     3361 tccggcatgc cgtaaatttc ctggttaatg tccggattca tcaggtggca cactttgcca
     3421 gggcgaaact ggaacgcgtc cttgccatcc tgcacatacc accatgattc aagatcgctt
     3481 ccgcgtcgca tgtatttcgc cagtgcgtgc cgtaatttaa gcggttcgcc gagcatattg
     3541 cttcgaagct caaggaatgc gttaccgaac acaaaccagt ccagcgccag cgccgagaaa
     3601 tcctgccggg aaagcagcgg gtgcgggatg tagcaaccga gtaatacatt gcgcttaaag
     3661 taaagcgcag actgatgcca ggacgtttgc cgggctgctc ttgccagacc gtaccagtcc
     3721 accggggttt cataccaccg cccgttatca gcacagtaca tattgtccag caggtcatgc
     3781 ccggtcaggc gataaggacc atcaaatgtg aatgcactga gcgatgattc tttcctgagc
     3841 gcatcagcga gatcaatgcg tgaactcatg cgcacttttt tattttttct gctcatcaga
     3901 actccataac cgtgaaacgc tcgttttctc cttcgccgcc aatcggttcg ttaatgacag
     3961 caagcatggt tgcccacgca aggtcgccgt ggctgatccc cctcgcgcgg tccgtttcgt
     4021 aagtgataaa gccgcccggt gttttcacct tacgcacggc gttaaaggcc gcgaccagct
     4081 cgcgttcggc gcgatcgtat tcccaccgcc cggcacgcat tatttgcagc attttcagta
     4141 ccagcgaccg ttttgatgac agcgtgaagg tgtacggaat agcagcaggg aaaaaccgtt
     4201 tcactatctg ataaacagcc tccccgttcc cgcccgtcac atcaatgccg atgtgttcca
     4261 cgttgtagcg atacgtgaac tcttcaatga ctctggcctg ttcttcaaac tccagcccct
     4321 gaacgcgtcg cgtctccacc gttcgaaaac ggccaccagg aacagccgga ggaaccacca
     4381 cggacacagc gccgctgtcg ccgttaccac tgctgccgtt tgcgtcatac ccaatccata
     4441 ccggacgatt ccccatcggg cggggagcaa aaggtttcca gtctttccag tcgtcgtatc
     4501 cgtcaacacc gcagccaatc aggatattca ggttaaatgc cgattcccct tcgcggacaa
     4561 actcacacat atagagattg aggaactcgt cttcggtgtt ttcatcacga atttcgtcga
     4621 tatcggtgtg tttccagccg tgattgacca catcttccag cgtgacaatt tgccgccatg
     4681 tccggtcagg gcagataagc ccgttatgca gcgttttcca gtccacagaa aaacgctggc
     4741 gtttatgcgt ggcctttttc tcgttccagc ggtcgccgtt ccagtaggcg tatgcctcgt
     4801 gcgtttcggt ggatggcgtg gagaagtagg tgcgccgcag tccgctgagg gttgccatag
     4861 cgccagccac cttgcgcagt tcagcaaagc gactgaccca gaaaaattca tcaaaataaa
     4921 aattgcccgt ataggactgt gccgatgcag cagaagtgcc gagaaaatgc agctctgcgc
     4981 cgttggagag gatgatttta tcgccccctt tcagctccac atcaacttca gccgcagcct
     5041 tctgaataat gcttttaaac tggaacgcct gacgacgcga cgcagacaaa aaaatctggt
     5101 tacgctggta aggttgcgcc acatcgtcac gcagcgccat cagcagtgct tcctgtgcaa
     5161 aataccaggt cgccccaatc tgtcgggatt tcaggatcat cctgttacgt atcccggctt
     5221 ccctgcaaag ggtcagggag tcaaaccagc cccgctgatg ccactccagc ctgctgatga
     5281 ttttttcccg cagtgcggca atctgttccg gcgtgaaatg attcttgagc tttttcgccc
     5341 ggcctttctt tcctgcggcc atcacatccg gctggccatc atgcagcttt ttaagctgcc
     5401 gggtcagcag gtctatttcc ttaaagtcac cgcctgtttt attctgtttt tcagtaagct
     5461 ggatgaggcg cgcatcgatg gactgcgtga cacgctgcac gggtggcgtt tcatcccact
     5521 ggtcacgttt tttccacgca taaatcgtgt tcgggtttat tcccatcaga cgtgatattt
     5581 ctgcgggcgg ataaccctgc cagtaaagtt gccgcgcacg ctggcgcaca aaagcgtcct
     5641 gaatcattgc tccccctgag taattacagg aagattaccc gcgcgcgaaa ctgttctcct
     5701 taaccccctg ttctggccgt tttcttacaa caaaaaccct ttgtatcagc ctgttacgct
     5761 ttgccatcat gactgaagaa ccagtcagag gggcaaaaac tatggctaat gaaaaaaaga
     5821 catcccgcaa aaagtttcgc gtggctgtct ccggatcaac tgttgatggc cgtgaaatca
     5881 gtccggtgca tctgcgtgaa gccgccgaga acttcaaccc ggatgtttac gctgcccgcg
     5941 tgaacgttga gcactatctc tcgccatgcc cgtcaagcga attttccgca atgggcgatg
     6001 tcaccgcact gagtacggaa gacattacgg aaggtccgct ggccggacgt actgcgctgt
     6061 atgcagaaat cgaaccgacc gagcgcatga agcagcttgt cgcggacggc aagaaaatct
     6121 attccagtat cgaactgcac ccgcagttct ccgttaacgg gcgcgcctat ctggtcgggc
     6181 tggcgatgac cgacaccccg gcaagcctgg gcactgagcg cctgaaattc acggcacagc
     6241 aacgtcaggc ggtgatgacg ttcaacagta tccagggtga agcgccgctt atctccgaag
     6301 ccatcgagtc tgaaatcatc gaaatggcag aacaacgcca ggaagaaggc acccagtggt
     6361 ttaaccgcgt aatggggatt attggccgtg gccgcaaagc ggatgacgcc agtttttccc
     6421 gtattcagga agcggtggaa ggcgtcgcaa cgtcacaggc cgacattatc gaccgtttta
     6481 atgtgctgga aacccgccat cagcaggaca gccagaaaat tacgtcactg accacagagc
     6541 tggcagcact gaaggaaaaa ctgcgcacgc aggacggcga tccgcagaac cggttcaccg
     6601 caacaggtgc agcctccgac cagctggctg acttctgata agacaaagga gcaaattttt
     6661 tatgaatctg gtgatgtcag atattacccg caacaagctg ggttgctata tggcgcagca
     6721 ggcgtcgctt aataatattc cggtttccgc actggtatcg cgatttaccg tggaaccctc
     6781 ggtgcagcag cgttttgaaa acgcctcaaa ggaaagcacc gaatttacaa aaagaattaa
     6841 cgtgatcggc gtgaccgacc agaaaggcga aaaaatcctc ctggacacca ccgggccaat
     6901 tgcacgcacg aataccagtt atgacggcac aaaacgccgt aacccgaata acgtggttga
     6961 tctgaaaaac cgcaaatacc agtgcgaaca ggtgaactac gacacgttta tttcatatcc
     7021 gcagcttgat gcctgggcgg cacatcctga ttttcagtcc cgcatcagcg cacagattgc
     7081 ccgacaggtg gcgcttgacc gcatcatgat cggtttcaac ggcacgtctc acgcggatga
     7141 gtccaacttc agcaccaaca agctgcttca ggacgttaac gtgggatggc tggagcacat
     7201 cagaaccgac gccagcgaac gcgttatgaa tgacgtgacg ctgacctccc gcaacatgga
     7261 caacaccgtg gcgcacgcgg gtaagtatgc gaacgctgat gcactggtac aggacgcgcg
     7321 ttcatccctg ctggatgaat ggcacaagga agctgacgac cttgtggtga ttatggggcg
     7381 caacctgttt aactcgctgc gtctgcccgt gctgaacagc atcagcggcc agaatcccaa
     7441 tgcggaatta cttgccgggc agctcatcct gtcatcgcgc accattggcg ggctgggcgt
     7501 gttccttgcg ccgttcttcc cggatgcaac gatgctgatc acctcgttca acaacctgtc
     7561 gatttactgg cagaaaggtt caatgcgtcg cctgatgaaa gacgagccgg aatacaaccg
     7621 catcgccacc taccagtcca tcaatgacgc ttatgtcgtt gaagactatg gcaagtgcgc
     7681 gatggtcact ggcctgaagt tcgccgacag ctaatcaact c
//
LOCUS       DAZFDP010000198         7704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFDP010000198 DAZFDP010000000
VERSION     DAZFDP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            399..1406
                     /locus_tag="ACHJ1G_004529"
                     /pseudo
     CDS             399..1406
                     /locus_tag="ACHJ1G_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2026..3535
                     /locus_tag="ACHJ1G_004530"
                     /pseudo
     CDS             2026..3535
                     /locus_tag="ACHJ1G_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3586..3700)
                     /locus_tag="ACHJ1G_004531"
                     /pseudo
     CDS             complement(3586..3700)
                     /locus_tag="ACHJ1G_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3787..4263
                     /locus_tag="ACHJ1G_004532"
     CDS             3787..4263
                     /locus_tag="ACHJ1G_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4766262.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            4244..5396
                     /locus_tag="ACHJ1G_004533"
                     /pseudo
     CDS             4244..5396
                     /locus_tag="ACHJ1G_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            5459..6908
                     /locus_tag="ACHJ1G_004534"
                     /pseudo
     CDS             5459..6908
                     /locus_tag="ACHJ1G_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6975..7467)
                     /locus_tag="ACHJ1G_004535"
                     /pseudo
     CDS             complement(6975..7467)
                     /locus_tag="ACHJ1G_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7390..7620
                     /locus_tag="ACHJ1G_004536"
     CDS             7390..7620
                     /locus_tag="ACHJ1G_004536"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4766263.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
BASE COUNT         2052 a         1836 c         2043 g         1773 t
ORIGIN      
        1 aggcagccta gcggctgcgg cttgtcatgg cctgaaatta ccgttataaa aacaaacaat
       61 gtcattgtct ttcaggtagt tatatggcac gttcagctaa accccgtaaa cgaaaactcg
      121 cacctcaaag aagcaaactt ccccgctatg ttgtaaaact tcacgaagat gatttttttg
      181 acgaagaaga agtagagatt ctgcgcttcg ataattttga cgatactgtt gagtgttgcg
      241 cagacctgaa tattccgttc tttgtggatg ccggaaacaa aaagctggtc ttctggtttg
      301 tacgtgttga tgacgaaggg catccggaaa tcgcccgctg tacagagcgg gagtttacga
      361 ccattcttgc cggtatcagc gccggcggca tgtactgccc ggactgttgc aatacaaaga
      421 aggtctgttt tcggtgtaaa aaacgctcct gcccacactg cggggtgaaa gcgggtgcac
      481 agtggataca gtatctgctg agtctggtcc ccgacggccc gtggcagcaa ttgtattcac
      541 gcttccctgc cagtactggt ccctggtgtt ccacaaccgg tggttactgg cagagatgag
      601 ccgcatagca gcagatgtga agccggggat attcacggta atccacacat ggggccggga
      661 ccagcagtgt catccacata tccacatatc catttatcca tttatcgaca actgccggcg
      721 gtgtgacatc acatcacacc tggaaaaacc ttcattttta cgcccgtaag gtgatgagta
      781 tgtagcgata ccggataaca cggttactgt cacggaaata cccggaacta ttgataccgg
      841 atgagctggc tgctgaagga agcagtaaac gggaatggaa tcgtttcctg gacagtcatt
      901 accgacgggg caggaatgtc aacgtatcaa aggtgatgga taacgccaca catgtggcgg
      961 tgtactttgg ctcttacctg aaaaagccgc cggtgccaat gagccgtctg gagcactatg
     1021 ccggtcagga tgaaatcgga ctgcgttaca acagtcaccg cacacgacgt gaagaatacc
     1081 tattgatgag tggtgatgcg tttatggaaa ggttctcctg gcatgtggcg gataaggggt
     1141 tccgtatggt gaggttctac gggttcctta gtccggcgaa acgccggttg ctggaagaat
     1201 tagtgtacgt aataacagag acggtgataa aaacggcgat gcaaatcagg tggagaggga
     1261 tgtatcagcg gttactgaag gttgacccgc tgaaatgcat cctgtgcgaa agtcagatgc
     1321 ggtttacggg gctgaagcgg ggttaccgtc tggcagagct ggttatgatg catgagccgc
     1381 tggcccgaat gcagtattgc ggctgagagc cgcagagggg aagttgcgtc cattttgagg
     1441 gaaatggagc aaaaaaacgt caccgatacc ctgtatcaat caatgtcagc aatataagcg
     1501 ctgatatata cccgatgagg ttccggatgt gttgaataaa tgttcgtttt ttgttttctt
     1561 aatctttccg ggggataaat tcctgtcgat gaacatatca aaatcaggaa attacacgaa
     1621 tctatgtaca ggctctctgg gaacacttta cgctttgaca cagagggcta aataatgagc
     1681 gattcagatg ccatttttgc atacagagga ttgacagcat tcccatgcta attgttaagg
     1741 tttaaattga agtatatcta tctgtcattt ttgcaaatga catgcggtag cataacgttc
     1801 cgaggtctct tatgcagaca tacgtggcga cattaaccac tcccagatac agccagcctt
     1861 catccgtacg taagtacgtg atgtctcgcg cccacttctg gctctggcta ctggcgtaaa
     1921 aatcctgctc ctgtcacgaa cggtgcaaaa gtggttcttc cgcaacctgg gtggagaaaa
     1981 tggatgaaac tttatggtga acaaggatct aagctgtttt tagttatggg taacgcaatg
     2041 cactctctta aaacacttct acagttacct tgcggatggc gatgtagtcg acaaattatt
     2101 aactctgacg gtatacccct ccatctccgc ggaaaacgca aaacagcaca atgtcctgaa
     2161 tgctttaagc gtagcgactc tgttcatagt tgtcgtcggc gccggataca gcatctaccc
     2221 tgctcagggc agacgctatg gcttgtattt tccgtccgcc acaggtacag ccgtaacccg
     2281 gcttgttcac gaaaaatttt tgccgagtcg tttgttccct tcggtggttc acatctgcag
     2341 tcttcacagg cgttacaaaa tttacagcgt caactgggat taatcgccgg aggtgaggcc
     2401 ggaaaacggg cagcaacggc agtgggtctc cgttgcagtg ctgatactct ccttcgcagg
     2461 gttatcaata ccccggagac gaaacagtca ggcgcgcctc atgtcggtat tgatgagtgg
     2521 gcgtggcatc ggggccaccg ttacggtacg ttaatcgtca atcttgatac tcaccgtccc
     2581 ctcgtcctgc ttcccggccg tgatcagcgt acgctggcga cctggttcag aaaatatccg
     2641 gaaatacaga ttgtctcgcg tgatcgcagt ggggtctgtg cgacagcagc acgtgaaggt
     2701 gtaccacagg ccagacaggt ggtcgatgcc acctgctaaa aaatattggc gatgcgcttg
     2761 aacgaatgat gtacagacat atacctctga tacgtcttgt tgccagcgag ttgtcaccaa
     2821 agaaaccacc tgagccagaa ctgtctgagc ctgcagcatc gctccgtcgt ccggaacgcc
     2881 taaaacaaca aacccgcaaa aaacggcatc agcgttggac agaggttatg gcccagcata
     2941 acaagggatg tagtttcagg gaaatatccc gtattacagg cctgtcgcgt gtgacagtca
     3001 gtcgctgggt gcgttcagga acattccctg aaatgtcaac ccgacctcca aagcgagggc
     3061 ttctggaccc atggagggag tggttaaaag agcaacgaga aagtggtaat tataacgcca
     3121 gccggatatg gcgggaaatg gtggcccggg taagccgctg gttgatgccc tggagaataa
     3181 ccagggatga agaaaattat gcttcccgat ttattagtct gatgtgtgaa aagaacctga
     3241 gctgaaaata gcgcagcaac tggcactcga gttctaccgt attctgaaaa cccaaaataa
     3301 atcacagcta agtagttggt tcactcgagt ccacgaaagc ggttcagcag aatttcggcg
     3361 agtggcggcg gggatggaag ctgatgctgc agctatatgt gaggcaatca gtagtcgctg
     3421 gagtaatggt gttgtcgaag gtcatgtaaa tcgcctgaag atgctgaaac gccagatgta
     3481 tggtcgagca ggatttgaac tactcaggca gagggtaatg agtccactgg catgaaaatc
     3541 tccacccagg ttgcggaaga ggcacttttc atccgtcgtt gacagctcca gcagattttc
     3601 tgacacaggc aggctgtgtg cggtaagggc tgaacttccg ggaagccttt gccctcagtc
     3661 cgtgacggca caggcttgcc atcacggttt ttacgttaaa agtaagcgta aaccgaccgc
     3721 agtatgtagc cattagacaa gaattggtaa cttagacgcc catctgacac agacggacat
     3781 ccaagtatgg aattacagga ctggcgaaaa gaacctcgta aaaagtattc gaatgaattc
     3841 aaacttcgta tggttgaact ggcatcacaa cccggtgctt gtatggccca gattgcacga
     3901 gaaaatggcg tcaatgatga tgttattttc aaatggctca gactctggca aaacgaaggg
     3961 cgtatatcac ggcgtcttcc ggtgacaacc acttctgacg caggcgttga attattacct
     4021 gtagagataa cgcctgatga gccgaaagaa ccgatggctg ctcttactcc atccttatcc
     4081 actcagacta ctgtcagtgc cagctcctgc aaagtggagt tccgtcacgg taacatgacg
     4141 ctggaaaatc cttcaccaga gctgctcact gtgaagtggc acactgaatt tggccacctg
     4201 aacagaggtg atatgctcac ctcagaacaa cacaggtgct ctaatgaaaa aaagaaattt
     4261 tagcgcagag tttaaacgcg aatccgctca actggttgtt gaccagaaat acacggtggc
     4321 agatgccgcc aaagctatgg atgttggcct ttctacaatg acaagatggg tcaaacaact
     4381 gcgtgatgag cgtcagggca aaacaccaaa agcctctccg ataacaccag aacaaatcga
     4441 aatacgtaag ctgaggaaaa agctacaacg cattgaaatg gagaatgaaa tattaaaaaa
     4501 ggctgccgcg ctcttgatgt cagactccct gaacagttct cgataatcgg gaaactcaga
     4561 gcgcattatc ctgtggtcac actctgccat gtgttcgggg ttcatcgcag cagctacaga
     4621 tactggaaaa accgtcctga aaaaccagac ggcagacggg ctgtattacg cagtcaggta
     4681 cttgagctac atggcatcag ccacggttcg gccggagcaa gaagcatcgc cacaatggca
     4741 acccggagag gctaccagat gggacgctgg cttgctggca ggctcatgaa agagctgggg
     4801 ctggtcagct gtcagcagcc gactcaccgg tataaacgtg gtggtcatga acatgttgct
     4861 atccctaact accttgaaag gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt
     4921 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
     4981 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
     5041 aaagcgcggg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
     5101 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
     5161 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
     5221 aggagtctga agagaacgaa tggatgccgg tggtgggtta cgtaagcttc agcgaggcag
     5281 ctcacgccat aacggactat atcgttggat attacagcgc actaagaccg cacgaatata
     5341 acggtgggtt acccccaaac gaatcggaaa atcgatactg gaaaaactct aactaggtgg
     5401 ccagtttttg ttgaccactt cagaaagcca gttccgtcag aaagaccagc aactaagtct
     5461 ggttgaagag acggaggcct tcctgtgctc tgcactggca cgtgccgaag aaaagatcga
     5521 agaagatgaa cgggaaatag aacatctgca gactcagata gaaaaactgc gccggatgct
     5581 gttcggtacc cgttctgaaa aactgcgtcg tgaagttgaa caggctgagg ccctgctgaa
     5641 acaacgcgaa caggacagcg atcgttacag tgggcgggaa gacgctccac aggttccccg
     5701 ccagttgaga cagccgcgcc atcgtcgtcc gttaccggca caccttcccc atgcaataca
     5761 ccgtctggag tctgaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctgga
     5821 ggaagtcagt gcagaacaac tggaactggt gagcagcgcc ctgaaagtga tccgcactgt
     5881 tcgggtaaaa aagcctgtac aaaatgtgac tgcatcgttg aagcaccggc gccgtcccgc
     5941 ccgatagagc gtggtatcgc gggccccgga ttacttgccc gcgtgttaac gggaaaatac
     6001 tgcgaacagc tgccactgta tcgtcagagt gaaatctttg cccgacaggg tgtcgaactg
     6061 agccgtgcat tactctccaa ctgggttgac gcgtgctgcc agttaatgac tccgctgaat
     6121 gatgccctgt accgttatgt gatgaatacc cgcaagcttc acactgacga cacaccggta
     6181 aaggtactgg cactgggcct gaaaagacga aaacagggcg catctggacg tatgtccgga
     6241 atgatcgcaa tgcgggttcg tcatctcctc cggcggtctg gttcgcgtac tcaccgaacc
     6301 ggcaggggaa acacccggag caacacctcc gccccctgca ggcggatgcg ttcaacggtt
     6361 acgatcggct gttcagtgca gaacgtgaag atggtgcgct gacagaagtt gcgtgctgag
     6421 cccatgtccg gagaaaaatc cacgatgtat acatcagcag caaaagtgcg acggcagaag
     6481 aagccctgaa gcgcatcagt gaactgtacg ccatcgagga tgaaatacgg ggattaccag
     6541 agtcagagcg tcttgaagtc aggcagcagc gaagcaaagc gttactgacg tcgctgcatg
     6601 aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagaccaggc gaagcgttca
     6661 gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt ctggcggagg
     6721 cggacaataa cacagcggaa agagcgcttc gtgccgtctg tctcgggaaa aagaattaca
     6781 tgttcttcgg tagcgatcac ggcggcgagc gtggagcact gctgtacggg ctgatcggca
     6841 cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg agcgtactac
     6901 cggaatagcc ctccaaccgt gttgacgaac tcctgccatg gaacgtagta ctcaccaata
     6961 aataagcgtc aatacggtgc tccgtagcgc tgttttgacc ggataaaagc cgcgagtacc
     7021 acgctgtcgc agtgctgacg gaactgctga catgtactta tccttgtccg ccgctgacac
     7081 cacatatacc agtcactgcg gccttcctgg agcacatggc acattgcctt gatgttgaac
     7141 acagcctgat gtttttcaat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg
     7201 cggccttttg gaggatggtc agctcttcat cccgttccgc cagctggcgt ttgagacgtg
     7261 caacatcggt agacatctcc agctcacgtt cagaagaacg tctactgatt ttgttgttga
     7321 ctacccagtt gtagactgtg attcatacag gctgagctca cgggctgcag cagcaatacc
     7381 tatactgttg tgaattgctg gcgctgcttc cctctttttt tcattggtat attgaccact
     7441 gacgaatggg gtagtcacat cagatatgtt atagaaggag actcatctat ttttattcag
     7501 catattgagt gcatgagaat attaatcaaa catctgaccc gtagggctat ctgtttcccg
     7561 cactctgtaa atttcataaa aaaacaccgg tactttatcg aaaaatacat gttctactaa
     7621 ttggatatca ccaaatcata taaaataatg tgttttatat aacaacctca aaatacattt
     7681 gaggttgtca ccaatcaaaa ttca
//
LOCUS       DAZFDP010000199         7640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFDP010000199 DAZFDP010000000
VERSION     DAZFDP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..551
                     /gene="ykgH"
                     /locus_tag="ACHJ1G_004537"
     CDS             <1..551
                     /gene="ykgH"
                     /locus_tag="ACHJ1G_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO4766264.1"
                     /translation="LYINKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVI
                     QRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLS
                     IVISVMFCLVKKMVDEYQQNSG"
     gene            complement(797..1492)
                     /locus_tag="ACHJ1G_004538"
     CDS             complement(797..1492)
                     /locus_tag="ACHJ1G_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO4766265.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1485..2912)
                     /locus_tag="ACHJ1G_004539"
     CDS             complement(1485..2912)
                     /locus_tag="ACHJ1G_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO4766266.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2923..3642)
                     /locus_tag="ACHJ1G_004540"
     CDS             complement(2923..3642)
                     /locus_tag="ACHJ1G_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO4766267.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4169..5023)
                     /gene="rclR"
                     /locus_tag="ACHJ1G_004541"
     CDS             complement(4169..5023)
                     /gene="rclR"
                     /locus_tag="ACHJ1G_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO4766268.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5249..6574
                     /gene="rclA"
                     /locus_tag="ACHJ1G_004542"
     CDS             5249..6574
                     /gene="rclA"
                     /locus_tag="ACHJ1G_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO4766269.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6683..6919
                     /gene="rclB"
                     /locus_tag="ACHJ1G_004543"
     CDS             6683..6919
                     /gene="rclB"
                     /locus_tag="ACHJ1G_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO4766270.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6931..7524
                     /gene="rclC"
                     /locus_tag="ACHJ1G_004544"
     CDS             6931..7524
                     /gene="rclC"
                     /locus_tag="ACHJ1G_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO4766271.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
BASE COUNT         2100 a         1847 c         1658 g         2035 t
ORIGIN      
        1 ttctgtatat caataaagat aatataaaag tgagttacag cctaaaaata aaccagacaa
       61 caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag accacaatga
      121 ctgaagatgt tatacagcga attactacat tttttcacac cagcccagat gtcaagaaca
      181 gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa
      241 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggc
      301 aagttctcga tgagatacaa acaccttcag caattaacag tgagctttat acaaaaatga
      361 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
      421 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
      481 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
      541 actctgggta gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
      601 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcat tgtaaatatc
      661 ctggcattac taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt
      721 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc
      781 agatctcata tctctctcag caatcctcaa taatcagata caccgctttc accggaccat
      841 gaaccccgac gactttgata agctcaatat ccgccgttga actagggccg ctaatgatgt
      901 taatgcagga cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
      961 gcgctacacg cggcaagata gtgcttttac gcaggataaa aagagaagat tccgggagca
     1021 ggctcaatga acgcccgcgc ccggcggcgg aaaaaagaac cacgcctccc gattcggtta
     1081 aaccatattc agcatacacg acacccactt tagcctgctc ggcctgcgag atattctcgg
     1141 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc
     1201 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     1261 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     1321 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     1381 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     1441 ggcgacccag tgcctgagca acgttattca aaaattcgcc tcgattatcc attctttttc
     1501 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     1561 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     1621 ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga
     1681 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatacctttt
     1741 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     1801 acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     1861 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     1921 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     1981 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     2041 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg
     2101 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     2161 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     2221 agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga acctgtctct
     2281 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt
     2341 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     2401 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     2461 gatggcggat cttgatccag ctggagaatg tattcaccca gatcggtttc aatcacctga
     2521 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     2581 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg agtggcgtct
     2641 tctttagttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     2701 tacagataag cgtcgagatt acttagaaca tgatcacgta tctgggcggc ccgatcgcgc
     2761 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     2821 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     2881 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     2941 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     3001 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac
     3061 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     3121 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     3181 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     3241 atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     3301 aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcagg
     3361 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     3421 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     3481 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     3541 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     3601 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     3661 tcatgcccat ttatgcccga tgtttccccc ccaccggggg atcaatcatc aataaatatg
     3721 gcgttctttt ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc
     3781 tttcatccaa acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt
     3841 atggggaaat cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt
     3901 atacctattc agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa
     3961 aaatcaccga caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat
     4021 ttagtttgca tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc
     4081 ccaaaccacc gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta
     4141 tctttgccct gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat
     4201 attctcccgg agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg
     4261 catagcctac tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg
     4321 ccgctatttg tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac
     4381 ggaaaagctg ggcaaaactt gcccgggaca tgtgagcgat gctggccagc gattcgacgg
     4441 tccaggcgtg tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca
     4501 gaagcaaact gagaatgttt ttctcagtat taacctgtgc aatccactca cgcaccgcga
     4561 gggtaaagaa tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc
     4621 tggattcctg ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt
     4681 taaccggtgc taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt
     4741 gcaaccgaag agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat
     4801 gagctgaatt ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg
     4861 tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt
     4921 ccccggcacc atgtggaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc
     4981 cttgtggagc gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg
     5041 tttttgatta caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt
     5101 aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca
     5161 aaatatcaag cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca
     5221 aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtga ttattggttt
     5281 tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct
     5341 catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac
     5401 caaaacattg gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa
     5461 aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa
     5521 tatcgacgtg atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg
     5581 gcctgaggca aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac
     5641 cgtggttccg ccaattcctg gaattaccac cacgccagga gtatatgaca gcaccggatt
     5701 acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt
     5761 tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc
     5821 gctgtttttg cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga
     5881 tcagggcgtc gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca
     5941 agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg
     6001 tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg
     6061 cggggcaatt gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg
     6121 agatgttacc ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg
     6181 tgatgagttg ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc
     6241 cgtattcatg acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag
     6301 tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat
     6361 gaatgatact cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg
     6421 ggcatcactg ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga
     6481 tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga
     6541 atcactcaat gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat
     6601 attctggcgt ctcagagaaa agaatcttat taattccctg ttactctata acttcttaat
     6661 cacttcattg atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc
     6721 tggcgttatg gcattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt
     6781 ctcgtcagta tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa
     6841 atataatgct tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac
     6901 agcagtattg tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg
     6961 ggagataaaa ttggcctgac attgattcgt ctgagtattg caattgtttt tatgtggatt
     7021 gggttattaa agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt
     7081 ccactaatgt cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa
     7141 ggcgaataca aaccagaagc aagggcatgg caaacggcca ataataccta tggtttttcc
     7201 aacggtcttg gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat
     7261 cgctggttag gtttattggg aggactgatg gcatttacca cgccgctggt aacactctca
     7321 tttttaatca ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc
     7381 ccttatttat ccggtgctgg tcgcctggta ttgaaagata ctctgatgct ggcaggtgct
     7441 gtcatgataa tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt
     7501 tcaacgttaa aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc
     7561 gtcaggcccc ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg
     7621 aagatcaata tcactcccct
//
LOCUS       DAZFDP010000200         7639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFDP010000200 DAZFDP010000000
VERSION     DAZFDP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..290
                     /locus_tag="ACHJ1G_004545"
     CDS             <1..290
                     /locus_tag="ACHJ1G_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4766272.1"
                     /translation="LIRSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSI
                     YIRQKYGHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            552..2174
                     /gene="yhiJ"
                     /locus_tag="ACHJ1G_004546"
     CDS             552..2174
                     /gene="yhiJ"
                     /locus_tag="ACHJ1G_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4766273.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2364..2477
                     /gene="yhiY"
                     /locus_tag="ACHJ1G_004547"
     CDS             2364..2477
                     /gene="yhiY"
                     /locus_tag="ACHJ1G_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4766274.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2540..3607
                     /gene="yhiI"
                     /locus_tag="ACHJ1G_004548"
     CDS             2540..3607
                     /gene="yhiI"
                     /locus_tag="ACHJ1G_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4766275.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWGRVNEELPWPDDLVVRLPQ"
     gene            3604..6339
                     /gene="rbbA"
                     /locus_tag="ACHJ1G_004549"
     CDS             3604..6339
                     /gene="rbbA"
                     /locus_tag="ACHJ1G_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4766276.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6339..7463
                     /gene="yhhJ"
                     /locus_tag="ACHJ1G_004550"
     CDS             6339..7463
                     /gene="yhhJ"
                     /locus_tag="ACHJ1G_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4766277.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1903 a         1802 c         2036 g         1898 t
ORIGIN      
        1 atctaatccg aagcaatatt atgaaaccca cagctgtata tgctgattat attaataaca
       61 ctactgattt cagaccaaaa cctgaacgta tcttcatatg tggagagaca ctcaaatgtg
      121 aagatcccag catatacatt agacaaaaat atggccatca tggggtcggg gtagatcaga
      181 atcaacaatt tgataatggt gttatggggc taaattccac gaagaaagta cttgataaaa
      241 ataacaaaat aatttattcc tcagggctta gttgttttca atctcattaa aactccatga
      301 atacggtggt atgccttttt aatcctgcca gtatttcata ggttttctta atcgattgta
      361 gaggtatgta ctgatgacgc ctctacaatc tttagggact ttctctgcta tgagataaga
      421 aatttcatat tatgctttaa ggctgaatga tttgtgcaaa atataattat ttttccatcg
      481 aacatgaggt gtatgtcata agattaaatg ttaactatgc attcctcgcg gtgaattata
      541 tgaggtaaaa gatgaaaatt ggaactgtgg caggcactaa cgacagtaca acgacaatcg
      601 caacaaatga tatggtgcaa gagcatgtta ctaacttcac taaagaactg tttgggtata
      661 ttgcaaatgg tataggtgat gatatatcaa gtattgccag gacaatgctc ggtgaagttg
      721 tggaaaaaat tgatgattgg caaatagaac gtttccaaca atctattcga gatgacaaaa
      781 tttcatttac catacaaacg aatcattcag aaaaatatag tatgctatcg ggcatgcgcg
      841 ctcatattct acgtagggat aataattatc agtttattgt gacaattaat agtataaatt
      901 acggctgcta tcttgataat accgatatta attggtgcag cattgtttat ttacttaata
      961 atatgacagt aaatgatgat gctaatgatg ttgcagttac tgagtcgtac aaacccatct
     1021 ggaactggaa aatatcgcaa tacaatgtat ttgatattaa attcgaaaca atgataaaac
     1081 cccagttcgc tgacagaacc tatttctcca attgttcacc tgttgatccc acaagtacca
     1141 gaccaaccta ttttggcgat accgatggct cagtcggtgc agttttgtac gcgctttttg
     1201 caacgggaca tttgggaatc atggctgaag gagaaaattt ccttagccag ttgcttaata
     1261 tagaagatga agttctcaac gtattgctaa gggagaactt taatgagcaa cttgacacta
     1321 atgttaatat aataatcagt atcctgaaca gaagagacat tgttttagaa agtcttcagc
     1381 cttatttggt tattaataaa gacgcagtca cgccatgtac gtttctgggc gaccagactg
     1441 gcgatcgatt tagtaatatc tgtggcgata aatttatcat cgatttatta aaacgcataa
     1501 tgagtatcaa tgaaaacgtt catgtattag ctggaaatca tgaaacgaat tgcaatggta
     1561 actatatgca gaacttcacg cgcatgaaac cacttgatga ggatacctac gctgggataa
     1621 aagattatcc tgtatgtttc tatggcccca aatacaaaat aatggcaaat catcatggga
     1681 taacttttga tgatcagcga aaacgttaca ttataggacc gataacagtc tctagtgatg
     1741 aaatgaccaa tgctctcgat ccggtcgaat tagcggcaat tatcaataaa aagcaccatg
     1801 ctgtaattaa tggcaaaaag tttaaaaata gccgggctat ctcttgtcga tcctttaatc
     1861 gttatttttc tgtttcgaca gattacagac ctaaacttga agcactatta gcatgctcac
     1921 aaatgttagg tataaatcaa gttgttgcac ataacggtaa tggtggaaga gaatgtatag
     1981 gtgaaacggg gacagtttta ggtcttaacg cccgtgacag taaacatgct ggaagaatgt
     2041 tcagcatgca taattgccaa attaaccctg gtgcagggcc tgagataacc actccctgga
     2101 agtcttacca gcatgaaaaa aacagaaatg gacttatgcc tttgattaga aggcgcacaa
     2161 tgttacagct ctgaaccata gacaacatga atattaatgt tactatctgg ttgagactgt
     2221 tgataaaacg taaaaaggaa tgctctgttc ctgcaactga acaggcgtga aacgcgatcc
     2281 aacaggatgg ataccgtttt gtcagccagt tttatagatc acacttatga gtatgcgtgt
     2341 ttcatttacg gccaggaatg ctgatgatga caacattact acccgttttc actaagcctt
     2401 ccccgttggc gctcaatgct ctgcgcgctg gtcgtatttg tcgtttcctt cttatcccgg
     2461 atggaagaat ccgctgaacg tcgttcggcg gcagttgtaa cacgcgtcgt ttgcttacag
     2521 ggattatggc gggccgatta tggataagag taagcgccat ctggcgtggt gggttgtcgg
     2581 ggcactggcg gtggctgccg tcgtggcgtg gtggctgttg cgcccggcgg gtgtgccgga
     2641 aggctttgct gtcagcaatg ggcgcattga agcgacggaa gtggatatcg ccagcaaaat
     2701 tgccgggcgt atcgacacca ttctggtgaa agaaggccag tttgttcgcg aaggtgaagt
     2761 gctggcgaag atggatactc gcgtgttgca ggaacagcga ctggaagcca tcgcgcaaat
     2821 caaagaggca caaagcgccg ttgccgccgc gcaggctttg ctggagcaac gacaaagcga
     2881 aagtcgcgcc gcgcagtcgc tggttaatca acgccaggca gaactggact ccgtagcaaa
     2941 acgtcatatg cgttcccgtt cactggccca acgaggggct atttctgcgc aacagctgga
     3001 tgacgaccgc gccgccgctg agagcgcccg agctgcgctg gaatcggcaa aagctcaggt
     3061 ttctgcttct aaagccgcta tagaggcggc acgtaccaat atcattcagg cgcaaacccg
     3121 cgtcgaagcg gcacaagcca ctgaacggcg cattgccgca gatatcgatg acagcgaact
     3181 gaaagccccg cgtgacggac gcgtgcagta tcgggttgcc gagccagggg aagtgctggc
     3241 ggcaggcggt cgggtgctga atatggtcga tctcagcgac gtctatatga ccttcttcct
     3301 gccaaccgaa caggcgggca cgctgaaact gggtggtgaa gcccggctga tcctcgatgc
     3361 cgcgccagat ctgcgtattc cggcgaccat cagttttgtc gccagtgtcg cccagttcac
     3421 gccaaaaacc gtcgaaacca gcgatgaacg gctgaaactg atgttccgcg tcaaagcgcg
     3481 tatcccaccg gaattactcc agcagcatct ggaatatgtc aaaaccggtt taccgggcgt
     3541 agcgtggggg cgggtgaatg aagaacttcc gtggcctgac gacctcgtgg tgaggttgcc
     3601 gcaatgacgc atctggaact ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc
     3661 cagcattatg gaaaaaccgt tgcgctgaac aatattactc tcgatattcc ggcccgctgt
     3721 atggtcgggc tgattggtcc ggatggcgtc gggaagtcga gcttattgtc gttgatttcc
     3781 ggtgcccgcg tcattgagca gggcaatgtg atggtgctgg gcggcgatat gcgcgacccg
     3841 aagcatcgcc gcgacgtctg cccgcgcatc gcctggatgc cgcaggggct gggcaaaaat
     3901 ctctaccaca ccttgtcggt gtatgaaaac gtcgattttt tcgctcgcct gttcggtcac
     3961 gacaaagcgg agcgggaagt gcgaattaat gagctgctga ccagcaccgg gttagcaccg
     4021 tttcgcgatc gtccggcagg gaaactctcc ggcgggatga agcaaaaact ggggctgtgc
     4081 tgtgcgttaa tccacgaccc ggaactgttg attcttgatg agccaacaac gggggttgac
     4141 tcgctctccc gcgcccagtt ctgggatctg atcgacagta ttcgccagcg gcagagcaat
     4201 atgagcgtgc tggttgccac cgcctatatg gaagaggccg aacgcttcga ctggttggtg
     4261 gcgatgaatg ccggagaagt gctggcaacc ggcagcgccg aagagttgcg gcagcaaacg
     4321 caaagcgcca cgctggaaga agcatttata aatctgttac cgcaagcaca acgccaggcg
     4381 catcaggcgg tagtgatccc accgtatcaa cctgaaaacg cagagattgc catcgaagcg
     4441 cgcgatctga ccatgcgttt tggttccttc gttgccgttg atcacgtcaa tttccgcatt
     4501 ccacgcgggg agatttttgg attccttggt tcgaacggct gcggtaaatc caccaccatg
     4561 aaaatgctta ccggactgct gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg
     4621 gttgatccaa aagatatcga tacccgccgt cgggtaggct atatgtcgca ggcgttttcg
     4681 ctctataacg aactcaccgt gcggcaaaac cttgagttac atgcccgttt gtttcacatc
     4741 ccggaagcgg aaattcccgc gcgagtggct gaaatgagcg agcgttttaa gctcaacgac
     4801 gttgaagatg ttctgccgga gtcattgccg ctcggcattc gccagcggct ttcgctggcg
     4861 gtggcggtga ttcatcgccc ggagatgtta atcctcgatg aacctacttc tggcgtcgat
     4921 ccggtggcga gggatatgtt ctggcagttg atggtcgatc tctcgcgcca ggacaaagtg
     4981 actatcttca tctccaccca ctttatgaac gaagcggaac gttgcgaccg catctcactg
     5041 atgcacgccg gaaaagtgct tgccagcggt acaccgcagg aactggttga gaaacgcgga
     5101 gccgccagtc tggaagaggc atttatcgcc tatttgcagg aagcggcagg gcagagcaac
     5161 gaagccgaag cgccgcccgt ggtacacgac atcacccacg cgccgcgtca gggatttagc
     5221 ctgcgccgtc tgtttagcta cagccgccgc gaagcactgg aactgcgccg cgatccggtc
     5281 cgttcgacgc tggcgctgat gggaacggtg atcctgatgc tgataatggg ttacggcatc
     5341 agtatggatg tggaaaacct gcgctttgcg gtgctcgacc gcgaccagac cgtcagtagc
     5401 caggcgtgga cgctcaatct ctccggttcc cgttacttta tcgaacagcc gccgctcacc
     5461 agttatgacg agcttgatcg tcggatgcgt gcgggcgata tcacggtggc aattgagatc
     5521 cctcccaatt tcgggcgcga tatcgcgcgt ggtacgcctg tggaactcgg cgtctggatt
     5581 gacggagcga tgcccagtcg tgccgaaacg gtaaaaggtt acgtgcaggc aatgcaccag
     5641 agctggttac aggatgtggc gagccgacaa tccacacccg ccagccaaag cgggctgatg
     5701 aacattgaga cgcgttatcg ctataacccg gacgtaaaaa gcctgccagc gattgttccg
     5761 gcggtgatcc cgcttctgct gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg
     5821 cgggaaaaag agcttgggtc gattatcaac ctttacgtga cccccaccac gcgtagtgaa
     5881 tttttgcttg gtaaacagtt gccatacatc gcgctgggga tgctgaactt tttcctgctc
     5941 tgcggcctgt cggtgtttgt gtttggcgta ccgcataaag gcagtttcct gacgctcacc
     6001 ctggcggcgc tgctgtatat catcattgcc accggaatgg ggctgctgat ctccaccttt
     6061 atgaaaagcc agattgccgc cattttcgga acggcgatta tcacgttgat cccggcgaca
     6121 cagttttccg ggatgatcga tccggtagct tcgctggaag ggcctggacg ttggatcggc
     6181 gaggtttacc cgaccagtca ttttctgact atcgcccgcg ggacgttctc gaaagcgctg
     6241 gatctgactg atttgtggca actttttatc ccgttgctga tagccatccc gctggtgatg
     6301 ggcttaagta tcctgctgct gaaaaaacag gagggatgat gcgccattta cgcaatattt
     6361 ttaatctggg tatcaaagag ttgcgcagtc tgctcggtga taaagcgatg ctgacgctga
     6421 ttgtcttctc gtttacggtg tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga
     6481 acctcgcgcc gatcgccatt gccgatatgg atcaatcgca gttatcgaac cggatcgtta
     6541 acagcttcta tcgtccgtgg tttttgccac cggagatgat caccgccgat gagatggatg
     6601 ccggactgga cgccggacgc tataccttcg cgataaatat tccgcctaat tttcagcgtg
     6661 atgtcctcgc cggacgccag ccagatattc aggtgaacgt cgatgccacg cgcatgagcc
     6721 aggcatttac cggcaatggg tatatccaga atattatcaa cggtgaagtg aacagctttg
     6781 tcgcgcgcta ccgtgataac agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta
     6841 acccgaacct tgatcccgcg tggtttggcg gggtgatggc aatcatcaac aacattacca
     6901 tgctggcgat tgtattgacc ggatcggcgc tgatccgcga gcgtgaacac ggcacggtgg
     6961 aacacttgtt ggtgatgccg ataacgccgt ttgagatcat gatggcaaag gtctggtcga
     7021 tggggctggt ggtgctggtg gtgtcgggat tatcactggt gctgatggtg aaaggcgtgc
     7081 tgggtgtacc gattgaaggc tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt
     7141 ttgccaccac gtcaatcggc atttttatgg ggacgatagc gcgttcaatg ccgcaactgg
     7201 ggctgctggt gattctggtg ctgctgccgc tgcaaatgct ttccggtgga tccacgccgc
     7261 gcgaaagtat gccgcagatg gtgcaggaca ttatgctgac catgccgacg acacactttg
     7321 ttagcctcgc gcaggccatc ctctaccggg gtgccggatt cgaaatcgtc tggccgcagt
     7381 tcctgacgct gatggcaatt ggcggcgcgt ttttcaccat tgcactgctg cgattcagga
     7441 agacgattgg gacaatggcg taaatacaat tgcccgatgc gtaagcttat agggactacg
     7501 catcggtgca atttattgaa ttttagttgt ttttaggccg gataaggcgt ttatgaactg
     7561 acccccaaaa cctggacagt ttcagttaac cggctttttg atactcagtc ctgtattcag
     7621 ggcaagatta cgaaagccc
//
LOCUS       DAZFDP010000201         7607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFDP010000201 DAZFDP010000000
VERSION     DAZFDP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1087)
                     /gene="oxc"
                     /locus_tag="ACHJ1G_004551"
     CDS             complement(<1..1087)
                     /gene="oxc"
                     /locus_tag="ACHJ1G_004551"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4766278.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEK"
     gene            complement(1141..2391)
                     /gene="frc"
                     /locus_tag="ACHJ1G_004552"
     CDS             complement(1141..2391)
                     /gene="frc"
                     /locus_tag="ACHJ1G_004552"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4766279.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(2904..3536)
                     /gene="yfdX"
                     /locus_tag="ACHJ1G_004553"
     CDS             complement(2904..3536)
                     /gene="yfdX"
                     /locus_tag="ACHJ1G_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4766280.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            3832..4107
                     /gene="ypdI"
                     /locus_tag="ACHJ1G_004554"
     CDS             3832..4107
                     /gene="ypdI"
                     /locus_tag="ACHJ1G_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO4766281.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(4184..4426)
                     /gene="yfdY"
                     /locus_tag="ACHJ1G_004555"
     CDS             complement(4184..4426)
                     /gene="yfdY"
                     /locus_tag="ACHJ1G_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4766282.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            4779..5699
                     /gene="lpxP"
                     /locus_tag="ACHJ1G_004556"
     CDS             4779..5699
                     /gene="lpxP"
                     /locus_tag="ACHJ1G_004556"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4766283.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            6055..6126
                     /gene="ypdK"
                     /locus_tag="ACHJ1G_004557"
     CDS             6055..6126
                     /gene="ypdK"
                     /locus_tag="ACHJ1G_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4766284.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(6191..7429)
                     /gene="alaC"
                     /locus_tag="ACHJ1G_004558"
     CDS             complement(6191..7429)
                     /gene="alaC"
                     /locus_tag="ACHJ1G_004558"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4766285.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         2009 a         1836 c         1700 g         2062 t
ORIGIN      
        1 atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata
       61 ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg
      121 caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa
      181 tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt gccaataacc
      241 aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg
      301 cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata gacattggca
      361 ggaatggaat ctgagtactt tcaataaact cacgaagctg ttcatcagct tgtgaatacg
      421 ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc
      481 tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact ttaacaatcg
      541 tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat
      601 aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat gcaatgccaa
      661 gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt
      721 ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc
      781 taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga
      841 ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg
      901 ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg
      961 cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa
     1021 tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat
     1081 ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc cccgttaata
     1141 tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg
     1201 caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg gcgtaaaggc
     1261 agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc
     1321 aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac
     1381 cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt
     1441 gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gtgcatgggc
     1501 tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc
     1561 ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt
     1621 acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc ggggaactgc
     1681 atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca atttatcgag
     1741 acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat
     1801 ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat
     1861 cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc
     1921 atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc
     1981 ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg
     2041 tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg
     2101 aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc
     2161 cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag
     2221 cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa cgccgggacg
     2281 ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga
     2341 ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc
     2401 tattagttaa ttaaatatgt gttaatttag cgtttattta aggcgtaata tcttctataa
     2461 ataacccgca aaatatcgtc gtagctgcac tcgatatttt taaaatcctg tatcaagtgc
     2521 tgaaaatcat ttcatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa
     2581 agaagaatat gataaatatt gattcgtggt aatcagagag agtcgcgaca tttgtgatgt
     2641 tattcttaca actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt
     2701 aaagcgtacc ataaaacata acataataaa tgatttgtga tgacacggga ttattgggat
     2761 ttactttgag gaagataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga
     2821 ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta
     2881 cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctgtc gacgatgatt
     2941 ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt
     3001 ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc
     3061 attacgccaa caccggccag gcgaagttct tccattgcgc cctttttatc ccctttcgcc
     3121 attttttcat tggcaatttt tatcgcggct tctttttcgg gagttgctac ataactctca
     3181 gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta
     3241 cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc ggaagcttca
     3301 ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca gagccaggcg cgctacctgc
     3361 acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg
     3421 gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
     3481 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
     3541 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
     3601 aaatcagaga gcaacggtca ttttgaaagg gttatcttac agttgtagtt attatcgcaa
     3661 gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg tcgcagagaa ctattcttat
     3721 gtaccacaaa gagatgtcat ttattatatc gatgtaaata ttgattaatt atatgtaaat
     3781 acagccataa catcgtaggt tctcagtgaa ccattttcag gataactttc tatgaaagta
     3841 aacttaatac ttttcagctt atttttattg gtctctatta tggcatgcaa tgtttttgca
     3901 ttttccattt ctggtggtgt aagtgagagg agctataaag agactgaaaa aacatcagcg
     3961 atgacaacca cacactctac aaaacttcag ccatcacagg cgattttgtt taagatgaga
     4021 gaagatgcgc caccattaaa cctcacagaa gaaatcacgc ctacttatcc gacaaaggcg
     4081 aattatctta ttcatcctgt gcgatagcgt ctgtcacatg gcaggtgaac aatcatgtct
     4141 gatgctgatg tgaacacctg cccaaatgag ccatcctgac ccactatcgc ctgccatacc
     4201 ccaccataaa gcgaatcatc aagccacaag ccggggcaag aaacataaat atccccgtca
     4261 ctagttcatt accgccagtt aaccacatgg tgaagtaata aatgagtgca gcgaggatca
     4321 ccatgccgaa gaaagaagag accgccgata ccacgttcag tacctgaccg atgtagccgc
     4381 ttacgcagac aatacacagg gcgagaaaca tccataaata aatcatagct caacctcctg
     4441 ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta agacaatcct taacctctgg
     4501 caatttttgc taagcatcaa tgaaaacagc atgttaaatg caagactgtt gtgtacggaa
     4561 aaatatttac tttgcacgat taataatcag tagctgaaag cagtcagcga gtgataaagt
     4621 acagataccg cattcatgct cctcgtggtt atgtcctgac tagtctttac actctttaca
     4681 ggaaccattg tcgtacatga tggcccaacc aattgagtgt accgctgtcg acatggaatc
     4741 ttctgcgata caacaattcg tatctacaga aggtaactat gtttccacaa tgcaaatttt
     4801 cccgcgagtt tctacatcct cgctactggc tcacatggtt tgggcttggt gtactctggc
     4861 tttgggtaca gcttccttat cctgttctct gctttctcgg cacgcgtatt ggcgcaatgg
     4921 cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc
     4981 cgcagcattc tgcggaagaa cgcgagaaga tgattgccga aaactttcgt tcactcggca
     5041 tggcgctggt agaaaccggc atggcatggt tctggcccga cagtcgcgta cgtaaatggt
     5101 ttgatgttga agggttggat aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg
     5161 ttgtcggcgt ccattttatg tcgctggaac tgggcggccg cgtgatggga ctgtgccaac
     5221 caatgatggc tacctatcgt ccacataata atcagctgat ggaatgggtg cagacccgtg
     5281 ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg
     5341 cactgaagaa aggtgaagcg gtatggtttg ctcccgatca ggattatggt cgtaaaggca
     5401 gctccttcgc gccgttcttt gcggtggaaa atgtcgccac aaccaatggc acctatgttc
     5461 tctcccgtct ctctggcgca gccatgttga ccgtaacgat ggtaagaaaa gcggattaca
     5521 gcggatatcg tttgttcatc accccagaga tggaaggcta cccgacagat gaaaatcaag
     5581 ccgctgccta tatgaacaag attatcgaga aagagatcat gcgcgcaccg gagcagtacc
     5641 tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga atcgtcgttg tacatttaac
     5701 aaactgctct tactctttcc ctcctcaaat cggccataat aacctcatgg tttttatggc
     5761 ttttttctta aggttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact
     5821 gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgt ctcttgtctc
     5881 gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt
     5941 gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta
     6001 tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa
     6061 tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg
     6121 atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg
     6181 acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt agtaaaccgt
     6241 cggcacggaa catcgcttta atcccacgaa tcgcctgacg aatacggtcg cggttttcaa
     6301 tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac
     6361 aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg
     6421 gttccgggat tttcgcccag acatacatcg aagccttcgg catttcaacc atccagcccg
     6481 cttcatgcag ccctttaacc aatacatcac ggcggcgttt gtactgttcg gcaatgtcgc
     6541 gcacgcactg ttgatcgccc tccagcgccg caatcgccgc cacctgcaac ggcgtaaagg
     6601 tgccgtaatc gtgatagctt ttaatacgag ccagggcgct gaccagcgtt ttgttgccaa
     6661 ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga
     6721 ctgccacatc gcgtgcaccc ggtacctgca tgattgacgg cgctttccag ccatcgtaga
     6781 cgatatcggc ataggccagg tcgtggacca ccagcacatc gtagcgtttc gccagcgcca
     6841 caaccttttc gaagaactcc agctccacgc attgcgcggt tggattcgac gggaagccga
     6901 ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga
     6961 aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat
     7021 aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg
     7081 ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg
     7141 ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg
     7201 gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt
     7261 tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat
     7321 cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg
     7381 ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag
     7441 attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa
     7501 tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggctttaaat
     7561 gaggaactcc acttcaaaaa caggaaattt ttctacgcta acaagaa
//
LOCUS       DAZFDP010000202         7452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFDP010000202 DAZFDP010000000
VERSION     DAZFDP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..843)
                     /locus_tag="ACHJ1G_004559"
                     /pseudo
     CDS             complement(20..843)
                     /locus_tag="ACHJ1G_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHJ1G_004560"
     CDS             complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHJ1G_004560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4766286.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHJ1G_004561"
     CDS             complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHJ1G_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4766287.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHJ1G_004562"
     CDS             complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHJ1G_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4766288.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHJ1G_004563"
     CDS             complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHJ1G_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4766289.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4808..5461)
                     /locus_tag="ACHJ1G_004564"
     CDS             complement(4808..5461)
                     /locus_tag="ACHJ1G_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4766290.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHJ1G_004565"
     CDS             complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHJ1G_004565"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4766291.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHJ1G_004566"
     CDS             6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHJ1G_004566"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4766292.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1834 a         1931 c         1893 g         1794 t
ORIGIN      
        1 gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa
       61 tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat
      121 caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc gccagtgtaa
      181 gtggatgccc ggctttggca aaggcatccg tgacgaattc cgaacaaaac cacgactttt
      241 tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg cactttggcg
      301 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
      361 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
      421 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
      481 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
      541 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
      601 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
      661 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
      721 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
      781 acaagccgaa agtaaaagaa agctggggag aaacaggcgg cagtacgcct ttggtttatc
      841 cattttatac aatccatgta aaaaaagggc cctgaaattc aggacccttt ctggcatcag
      901 cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa ctgtttctcg
      961 ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca
     1021 atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg
     1081 cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag tttctcagca
     1141 agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac ctggaacggc
     1201 gcgatagcgt caggccatac gatgcctcgt tcgtcgtagt tctgctcaat cgccgcagct
     1261 accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt acggccatct
     1321 tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga
     1381 ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct tggatcgcca
     1441 gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc ccagttgatg
     1501 ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat cgccgcaacg
     1561 gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct
     1621 ttaaccacgg cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc aacctgcggc
     1681 agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag cgcaaccagc
     1741 gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt ctcaatcggc
     1801 agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc
     1861 atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg
     1921 ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct ctgcgccagc
     1981 acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcttg tacggcgcgg
     2041 aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt
     2101 tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg
     2161 gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag
     2221 aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc agtgataact
     2281 tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt
     2341 tccggaccgt actgttccca acgaccactc tcttgccaca aatcagctgg ctgaaccacc
     2401 ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact
     2461 tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg
     2521 atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc
     2581 ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt
     2641 agaacaggct caaggcgagc ctgggacaaa aaaaagtgat ttagtttacc agtgcaaaag
     2701 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
     2761 gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata ggttttgccc
     2821 gtttcctcac ctttacgata ggccgggcgt gggtcctgcg ccagtacttc gcggataaac
     2881 agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac ctctgcggta
     2941 aaactcaccg ccatctctgc cgctggcgca ctttgcgcat agctggcact ggcatcggga
     3001 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
     3061 tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc tttcagctct
     3121 accagcgaca taccaattgg gttagggcgg aaagtagagc gtgttgcgaa aacccccatt
     3181 ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc ttccatcgtt
     3241 tgatgaaaga cgaaaaggat ccataaatgg ctgaacgctt ccaggccgcg aacggcgtcg
     3301 gcctggttgt agggagcaat gagatgcagt tcgccgttgg cgctttttac cagacctggc
     3361 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat ttgctcgaac
     3421 tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca gcctgacgat
     3481 agcagcctgg cgtaccgctg gtgacttcgc agctatgcag taataccgca ttggccttca
     3541 ttttcgaggc gttgatttgc atccgcttac gcgcggttgg aatgctcggc ggagagtcct
     3601 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
     3661 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
     3721 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
     3781 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
     3841 ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa atgacaagac
     3901 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa aattaccagc
     3961 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca tcagactgat
     4021 aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc gttacgatca
     4081 ggtttacgta cggggagtct ttatcttcga caaagatacc gtctttcgct ggagtcaggc
     4141 caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg tcgtccagag
     4201 atcgaggcag ctgcggtgct tccagctcaa caattttcag attttttggg ttctcaacaa
     4261 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
     4321 gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca acctgcgaac
     4381 catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc ggataaacaa
     4441 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
     4501 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
     4561 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
     4621 gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
     4681 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
     4741 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
     4801 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac tgaattaaat
     4861 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta tagccgatgt
     4921 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
     4981 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaatgctt
     5041 ccggtaacag cactttacgg acgatctgca tcggcgtggc acccattgca cgggaagctt
     5101 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
     5161 ctgcaccaac ggttaacgga acaatcgctg cctgcaaacc aatcgatgta ccgacaataa
     5221 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
     5281 tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc cccgggcgcg
     5341 taacataaag caggacgccg accggcagac caatcacaaa gccaaaaaat ccggatacga
     5401 aggtcattgc cagcgtttcc catacgccac gaaccagcaa ccacatcatc ggctcagaca
     5461 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
     5521 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc accggcgtaa
     5581 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
     5641 agcggggcat cgaccgattg accggtaaac tccaggcgca acatcggcac gcagtcagta
     5701 aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg cagggtcgac
     5761 tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact taccgtgtcc
     5821 tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg cttcacaacg
     5881 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat gtctttcagc
     5941 agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc atcacacagc
     6001 aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg
     6061 gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga cagcaattcc
     6121 gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg cagagccacg
     6181 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
     6241 cggcgagctt tggtcaactc ggattctgac agcgtggtca gttcctggcc atcgaccagc
     6301 acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt actcttaccc
     6361 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
     6421 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
     6481 atcattgatt atttattatc gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg
     6541 gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
     6601 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg ccttattaag
     6661 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
     6721 aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
     6781 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac caaccagtct
     6841 ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga gtggatggac
     6901 tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc gcatcatccg
     6961 cagggtagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg
     7021 cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
     7081 aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt
     7141 acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt aaatagcctg
     7201 gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cacaatgatt aaaagatgag
     7261 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc tataatgcgc
     7321 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
     7381 aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
     7441 acagtgcgct aa
//
LOCUS       DAZFDP010000203         7323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFDP010000203 DAZFDP010000000
VERSION     DAZFDP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(204..623)
                     /locus_tag="ACHJ1G_004567"
     CDS             complement(204..623)
                     /locus_tag="ACHJ1G_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO4766293.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            complement(707..1144)
                     /locus_tag="ACHJ1G_004568"
     CDS             complement(707..1144)
                     /locus_tag="ACHJ1G_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO4766294.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGR
                     REFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
     gene            1402..3495
                     /gene="cda"
                     /locus_tag="ACHJ1G_004569"
     CDS             1402..3495
                     /gene="cda"
                     /locus_tag="ACHJ1G_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFO4766295.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPA
                     AGNNVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKVELFSKAGV
                     KQPPVYTQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGM
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDA
                     GGLRDFIYWRPDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            3492..3755
                     /locus_tag="ACHJ1G_004570"
     CDS             3492..3755
                     /locus_tag="ACHJ1G_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO4766296.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDV
                     LNCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
     gene            3825..3971
                     /locus_tag="ACHJ1G_004571"
     CDS             3825..3971
                     /locus_tag="ACHJ1G_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO4766297.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTG
                     VAIQ"
     gene            complement(4200..4313)
                     /locus_tag="ACHJ1G_004572"
     CDS             complement(4200..4313)
                     /locus_tag="ACHJ1G_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4766298.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
     gene            complement(4983..5174)
                     /locus_tag="ACHJ1G_004573"
     CDS             complement(4983..5174)
                     /locus_tag="ACHJ1G_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4766299.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            5241..5564
                     /locus_tag="ACHJ1G_004574"
     CDS             5241..5564
                     /locus_tag="ACHJ1G_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO4766300.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5773..6207
                     /locus_tag="ACHJ1G_004575"
                     /pseudo
     CDS             5773..6207
                     /locus_tag="ACHJ1G_004575"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6260..6802
                     /locus_tag="ACHJ1G_004576"
     CDS             6260..6802
                     /locus_tag="ACHJ1G_004576"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO4766301.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVT
                     GGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            6809..7042
                     /locus_tag="ACHJ1G_004577"
     CDS             6809..7042
                     /locus_tag="ACHJ1G_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO4766302.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1816 a         1611 c         2068 g         1828 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc cacttactgt cttccctcgt agccatcgag ataaaccgca
      241 gaaatcgtct cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca
      301 gtacccgcaa attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgc
      361 tgaatgcgtt gctgcacatc actccgtgat tttcctgtcg ctttatcctt tgctcccata
      421 tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcggcctt ctgggtggaa
      481 aagttaggat caaaaccatc gtctgatgca taaacgtaat gccgggtatg ttgttccaca
      541 ctgccctttg gcgtgcaggc tgtcaggacc gttgccgctg tcagcagggc gataagatac
      601 agtgtgccgc cggaacgtct catgagtgtc tccttacctt taaatctttg tactcagtga
      661 cttatattta accgctgact atcttatcat cacttgcagc cgatgctcac cggctgaaca
      721 gtctggccca gaatccccgt tttttttcct gctccagtgc ctgcctgagc tgctctatct
      781 ctttatgtag ctgttcccgt tcggcttcct ggcgccggcg ttccgtatcc tgtgcctgtt
      841 tgtcctcaag catcagtgtc aggcattgtt tcagctcatt cagctcccgg agaatgcgtt
      901 ctgtctgctg atcatgtgga ttttctgcct gtcccgcact gtgcctttct gctgtctcat
      961 cctgctttaa ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgtcgtccgt
     1021 cctggccggt acggtaactg acaaggcctg cgcgcatatc acggtaaaac tgtgaacgcg
     1081 atctgcctgt cattttcagt gcgtcctgga gtgtgtgcca tgccatgaag tgacagtgtc
     1141 ccattggtgt ctcatatcat ggtttcagta aaacatactg gggccggaga acggtaatgt
     1201 ttgtgctggt ttttgtggca tcgggtggga atagcacgtg gtgtgggtgg ctgttttttt
     1261 gatcgttttc acaaaaacga aaatccacag ggttgacagg gaaaacgcag cggcgtaatt
     1321 ttaaactgta tataaaacca gtggttgtat atacagtatg cgtcttttaa ctatttgttt
     1381 ttaatgtcaa agaggttttc tatgagtgat aatgaaggta gtgtcccgac agaaggtatt
     1441 gattacgggg acacaatggt tgtgtggccg tcaactggac gaattccggg cggtgatgtg
     1501 aaacccggtg gttcatcagg tctcgctcca tccatgcctc cgggatgggg agattacagt
     1561 ccacaaggta tcgcacttgt acaaagtgtt ctttttccag gaattattcg ccggattatc
     1621 cttgataagg aacttgaaga gggggactgg tcgggatggt ctgtcagtgt gcatagtccc
     1681 tggggaaacg agaaagtttc tgctgcacga acagttcttg agaatggttt acgtagtggt
     1741 ttgccagaac catcacgccc ggctgctgtt tcttttgccc gtctggagcc tgcttccgga
     1801 aatgagcaaa aaattattcg tcttatggtt acacagcaac tggagcaggt aacggatatt
     1861 cctgtcagcc agttaccagc agcgggtaat aatgtaccgg tgaaatatcg tctgacggac
     1921 cttatgcaga acggtacgca atatattgct attatcggag gtattccgat gacagtgccg
     1981 gtagttgatg ccgttccggt tccggaccgg agccgtccgg gaacaaatat taaagatgtt
     2041 tacagtgccc ctgtatcacc aaatctaccg gacctggtat taagtgtggg gcagatgaat
     2101 actccggttc tgtctaatcc cgaaattcag gaagagggcg ttattgctga gacaggtaat
     2161 tatgttgagg caggttatac gatgtccagt aataatcatg atgtcattgt tcgttttcct
     2221 gacggcagcg gtgtttctcc gttatatatt tcaactgtgg agattcttga ccgtaatggt
     2281 ctgagtcagc gtcaggaagc agaaaataaa gcaaaggatg atttcagagt caagaaagaa
     2341 gaagcggtag cccgggccga agctgaaaaa gcaaaagtcg agttatttag taaagcaggt
     2401 gtgaaacagc ctcctgtata tacacaggaa atggtggaaa gggctaattc agtaatgaat
     2461 gagcagggaa ctcttgttct gaataacact gccagttctg tacaactggc gatgaccgga
     2521 actggtgtct ggacggctgc tggtgatatt gcaggcaaca taagcaagtt tttcagtaat
     2581 gctctggaga aagtaaccat acccgaagtg ggtcccctgc ttatgcggat ttctcttggc
     2641 gctctgtggt tccattcaga agacgccgga gcaggaagtg atatcgtgcc ggggcgagat
     2701 atggaggcta tgttctcgct gaatgctcag atgttggcag gacagggcat ggtcattgaa
     2761 cctggtgcta cgagtgttaa tctgcctgtt cgtggacaat tgataaacag taacggacaa
     2821 cttgctctgg atttactgaa gacagggaat gatagtatcc ctgctgcggt tcctgttctt
     2881 aatgctgttc gtgatacagc tacaggactg gataaaatta cgctgccagc agtagtcggt
     2941 gcaccttcca ggacgattct ggtaaatccg gccccacaac cttcggcacc aacagataca
     3001 ggaaatcatc aacccgttcc ggttacccca gtgcatacag gtacggaggt aaaaccggtt
     3061 gaaatgccgg taacgacgat tactccagtc tctgatgccg gtggacttcg agattttatt
     3121 tactggcgtc ctgatgctac cgggactggt gttgaagctg tctatgtgat gcttaatgat
     3181 cctctggatt cagggcggtt ttcacgcaaa cagcttgata aaaaatataa acatgctggt
     3241 gattttggta ttagtgatac taaaaagaat cgtgaaactc ttactaaatt cagggatgct
     3301 attgaggaac atttatccga taaggataca gtagagaaag gaacataccg acgagaaaaa
     3361 ggatcaaaag tttattttaa tcctaataca aataatgtgg tgatactaaa atcaaatggt
     3421 gagttcttat ctgggtggaa aataaatcca gatgcggata atgggcgaat ttatttagaa
     3481 acaggtgaac tatgaacaag atggcgataa tcgatttggc gaagtcattt ttagcttcaa
     3541 aaataacagc aatggagttt tcagagcgga tttgtgttga gcggagaaaa ttgtatggtg
     3601 ttaagaattt gtctcaggat gtattgaatt gtggggagga gttgtttata gctgctgagc
     3661 gtttcgagcc tgattctgat agggctgatt atgaattcga tgataatgga cttaggattg
     3721 aagtccaatc tatcctggaa aaatttaaat tataaatatg agtgatttta atatctggaa
     3781 tttaattata aatgtcagat actcactcat gacaaggaga tgttatgaaa aaaaagatag
     3841 gggtatcgat tattctcgtt cttgttgttt tgtgtctgac tgcttgtcaa aataactatg
     3901 taagggatgt tcagggagga acgattgctc catcatcatc ctcgaaacta accggggtag
     3961 cgattcagtg aaaaagatta aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4021 ctgcttgcaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg aacaagagct
     4081 accaactctt tttccgaagg taactggctt tagtggagca cagataccaa atgctgtcct
     4141 ttcagtgtag ccgcgataag gcttcaactt caagagttct gcgtatctaa atccactttt
     4201 cagttaccaa tggctgctgc cagtggcgta aagtcgtgtc ttaccgggtt ggactcaaga
     4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4321 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taatcggcag ggtcggaaca
     4441 ggagagcgca cgagggagct tccgggggga aacgcctggt atctttaagt cctgtcgggt
     4501 ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc gtcagggggg cggagcctat
     4561 ggaaaaagcg ccaccggcgc ggccttttcc ccggctttgt cttgttgctc acatgttctt
     4621 tccggcgtta tcccctgatt ctgtggataa cagtattgcc gcttttgtgt gagcagacac
     4681 cgctcgccgc agccgaacga ccgagcacag cgagtcagtg agcgaggaag cggaagagca
     4741 tcggcatctg catgttctcc ttacgcatct gtgcggcatt tcacaccgta catggtgcac
     4801 ttttcataca atcctcactg atgccacacg attaagcgag tatacactcc gctatcgcta
     4861 cgtgactggg tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg
     4921 gcttgtctgc tcccggcatc cgctcacagg caagctgtga ccgtctccgg gagctgcatg
     4981 tgtcagaggt tttcaccgtc atccccgaag cgtgcgaggc agctgcggta aagctcgtcg
     5041 gcgtggtcgt gaagggcttc gcagatgtct gcctggtcgt cggcgtccag ttcattcagt
     5101 ttttccagca ggatcagcgt ctggcttctg ataaagcggg ccatgttaag ggctgttttt
     5161 tcctgtttag tcacagttct gttctccatg tgtggggagt ctgtcggagg tttttaatga
     5221 taccggtgaa acgcgaaagg atgctcacga tacgggtcac ggatgaggaa catgcgcggc
     5281 tgctggcacg ttgtgaggga aaacaacttg cgtcatggat gcgtaaggtc tgcctgggtg
     5341 caccgccatc gaaaacgtca ggtcttccga cactggcacc gccgctgttg cgccagttcg
     5401 cttcggtcgg caataacctg aatcagatag cccggaagat aaacagcgga cactggtcgg
     5461 ggcatgaccg ggttcatgtg gtggctgcac tgatggccat cggacgtgaa ctgagtgagc
     5521 tgagggatga ggtccgtaaa cagggagaac gggatgatag ttaagtttca tgccagggga
     5581 aaaggcggtg gcagtggtcc ggttgattac ctgctgggcc gggagcgtaa ccgggaaggg
     5641 gcgagaattc tgcggggtga tcccgaagag gtgcgggaac tcatcgatgc cacgccgttt
     5701 gcgaaaaaat acacctcggg ggttctgtct tttgcagagc agacactgcc accgggagag
     5761 cgtgaaaggg tgatggagag ctttgagcgg gtactgatgc ccggtcttga aaaaaatcag
     5821 tacagcatcc tgtgggtgga gcaccaggac aaggggcgtc ttgagctgaa ttttgtcatc
     5881 ccgaatatgg agctggtgag cggaaaacgg ctccagccct attacgaccg tgcggaccgt
     5941 ccgcgaatca atgcctggca gacactggtg aatcatcatt acgggctgca cgacccgaac
     6001 gcaccggaga acaggcggac acttgtcacc ccgaataatc tgccgaaagc gaaacaggag
     6061 gccgcagaag cgattacgag ggggctggag gctctttatc atgccggagc gctaaaaacg
     6121 cgtcaggatg tcactgaggc gcttacagcg gcaggttttg aggtggtgag gacaaccagg
     6181 agcagtatca gtatcgccga cccggagggc gggcgtaatc tcaggctgaa aggggcgctg
     6241 tatgagcaat cttttacaaa tggggacaga cttcgagaag aaactgaaag agcgtgccgt
     6301 ctctacagag aacatgctga acagcgagtt caggaagctc gaagcgtctg tagacaggga
     6361 actgaactta aacgggcaga aaatcagaga cgctatcagc gcacacacag cagcgctgaa
     6421 agaacaactg gagacgctga acagtgccgt gaacgcgcag ttcagcgcca cagagaacga
     6481 actggcccgg cggcaggaag agctgctctg gaagctgatc aaagggcgga tactgtaccc
     6541 gtcgctgacg gcgctttgcg tcacgggcgg gatttttctg gcgtcatggg ggctgattca
     6601 gtggcaggaa agccggattg cggcgaacat cctggctatc agggaccagg aagagacgct
     6661 ggcgaaactc cgggaaaaaa catggggtgt gacgtatcac gagggcagga acgggaaatt
     6721 tctggtattg ccgtccgggg tgaaggggga aaacaactgg acggtggaga agaagaacgc
     6781 agtaagactg gtcagggagt aagtgagtat gacagagctg gaaatgcact tactgaacgc
     6841 cttagagcaa ttgcagcagg actatacgca gcggctgaaa gactgggaga gcgccttcac
     6901 ggatttgcag catatgttcg agctgacacg acaggagaac gggcaactga gggaacagtg
     6961 cgacgacttg agtcagcagg tgcagagatt agccgggcag gtggaccgct tgagtcggtt
     7021 atttatcacg aacagcagat aagggatgaa caggaaaggc tggcacgcct ggaacgggag
     7081 cgtgcgcggt cctggcatga agagcaggaa cgggaagtac gggtatggcg tggcccgtca
     7141 atggggatgt gacggtgagt tgttccggga ggttgccaga tggtaccgga acgcgttaaa
     7201 aatgcatccg ggcgcgattc tggcgtgtcg tttttgttgc tgtgttttgc ctctttgcca
     7261 gatcgcgctc attcctcagc ctcatcccgc gatgtctgcg tatgtcatca actgaaaaga
     7321 tgg
//
LOCUS       DAZFDP010000204         7301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFDP010000204 DAZFDP010000000
VERSION     DAZFDP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..278)
                     /locus_tag="ACHJ1G_004578"
     CDS             complement(<1..278)
                     /locus_tag="ACHJ1G_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO4766303.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
     gene            complement(448..524)
                     /locus_tag="ACHJ1G_004579"
     tRNA            complement(448..524)
                     /locus_tag="ACHJ1G_004579"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Arg,seq:tct)
     gene            complement(538..614)
                     /locus_tag="ACHJ1G_004580"
     tRNA            complement(538..614)
                     /locus_tag="ACHJ1G_004580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Arg,seq:tcg)
     gene            complement(624..699)
                     /locus_tag="ACHJ1G_004581"
     tRNA            complement(624..699)
                     /locus_tag="ACHJ1G_004581"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(664..666),aa:Ile,seq:cat)
     gene            complement(728..1441)
                     /locus_tag="ACHJ1G_004582"
     CDS             complement(728..1441)
                     /locus_tag="ACHJ1G_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766304.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1576..1773)
                     /locus_tag="ACHJ1G_004583"
     CDS             complement(1576..1773)
                     /locus_tag="ACHJ1G_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4766305.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(2015..2545)
                     /locus_tag="ACHJ1G_004584"
     CDS             complement(2015..2545)
                     /locus_tag="ACHJ1G_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4766306.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWNQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(2554..2913)
                     /locus_tag="ACHJ1G_004585"
     CDS             complement(2554..2913)
                     /locus_tag="ACHJ1G_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4766307.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(2926..3972)
                     /locus_tag="ACHJ1G_004586"
     CDS             complement(2926..3972)
                     /locus_tag="ACHJ1G_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766308.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(3974..4246)
                     /locus_tag="ACHJ1G_004587"
     CDS             complement(3974..4246)
                     /locus_tag="ACHJ1G_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766309.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            4382..4639
                     /locus_tag="ACHJ1G_004588"
     CDS             4382..4639
                     /locus_tag="ACHJ1G_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4766310.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            4645..4944
                     /locus_tag="ACHJ1G_004589"
     CDS             4645..4944
                     /locus_tag="ACHJ1G_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4766311.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(5149..5493)
                     /locus_tag="ACHJ1G_004590"
     CDS             complement(5149..5493)
                     /locus_tag="ACHJ1G_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766312.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(5490..5855)
                     /locus_tag="ACHJ1G_004591"
     CDS             complement(5490..5855)
                     /locus_tag="ACHJ1G_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4766313.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(5857..6075)
                     /locus_tag="ACHJ1G_004592"
     CDS             complement(5857..6075)
                     /locus_tag="ACHJ1G_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4766314.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            complement(6301..6798)
                     /locus_tag="ACHJ1G_004593"
                     /pseudo
     CDS             complement(6301..6798)
                     /locus_tag="ACHJ1G_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(6795..6971)
                     /locus_tag="ACHJ1G_004594"
     CDS             complement(6795..6971)
                     /locus_tag="ACHJ1G_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766315.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            complement(6964..7146)
                     /locus_tag="ACHJ1G_004595"
     CDS             complement(6964..7146)
                     /locus_tag="ACHJ1G_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766316.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(7180..>7301)
                     /locus_tag="ACHJ1G_004596"
     CDS             complement(7180..>7301)
                     /locus_tag="ACHJ1G_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766317.1"
                     /translation="NAAKNELEPWTPEGNAPNYCWWEVDPVDVANEAMSYHND"
BASE COUNT         1813 a         1955 c         1557 g         1976 t
ORIGIN      
        1 atctcgcgta acgcggcacg acgaccaatt ttaaaattgg tatccagctg accgataata
       61 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
      121 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      181 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      241 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacgttcct ttgggtgggt
      301 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      361 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      421 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtcgaa ccactgaccg
      481 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
      541 accgccatcg gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctgtcccga
      601 tgagctaatg gcggtatgtg atatggtggc ccttgctgga tttgaaccag cgacctggcg
      661 attatgagtc gctcgctctc accactgagc taaagggccg agccaaaaaa taataatcag
      721 atgaaaatca ataatcaagc ccttgcctgg atacatatct gtctggcggg aagccataat
      781 agcggtgaaa tacagaaata aagtaggacc tgcttgaata accgcatttt tctgctatag
      841 cctgtccata tccatgccgg gaacataaca tatttacagc aacacgcatc cgctcttcca
      901 gcaacaagcg actgaacatg cccccttcat ttttcagttt tgtctttaac aaactctcac
      961 tcatatgcaa ctgtagagca atcgcaccaa gcgtccagct tgctgatata tctgtctgaa
     1021 ttatcgccct gactttggca cttatgctgg ataaacatcc acttaaaaac aatgacatcc
     1081 gttcatctgt ttcaaacaga gacaggcagg ccatcataag aaacatatcc gtggcctctc
     1141 cggaaaatcc ctggctggta attaaagccg cagccaacgc aggattgttg ggttccagca
     1201 acaggtaaag cggaatgtca gtcagacgag tcctcgtcag cttatgctga ctttccagat
     1261 attgacttac gacggattcg cttatatcga caattttaac tttgccataa tgcataagga
     1321 aaagctccct gatgcatttg gtggccagaa cgactgagcc tggcttaagt gacaacgtat
     1381 ccttttcaag aaaaatatta attggggagc aaaccatgat aactgaacag acaacagcca
     1441 ttataatttt actttcatta gcaattggtt agctcaatta tagccccaaa aggtaaatta
     1501 tcatcaacac ataagcaaag gactgacagg tgtcgccccc ccaccagccg cccattcacc
     1561 acaaataaaa agccttcagg actgaaggcg tctgtaacaa ccgcactgat agtctgccag
     1621 acccgccata acaagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt
     1681 ctgcgcaacc tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct
     1741 ggcggtttcg gtcatatcct gctgtttcag catgtctttt tccctttttc ggttaacgtg
     1801 acacaccaat aactcttgtc gaaaaagcca gcaagctgaa agaacggtat taataaccac
     1861 cagcgaattt attgcgctgc tgtatattac ggacacaaaa aaaccacctt ccggtggttt
     1921 ccttgtgcga aaaaacttgc atttcgcctc gcgatacagc tttgcgaagc ttacaggaat
     1981 tcaagctgtt tctgcgtaaa aaagcaagct ttttttatcg aaatgaatcg tgcataggta
     2041 cataaagcat gtgttcagcc acggctaacc aacctgcaat acgtttctca catgtgctga
     2101 aacaccattc cgggtgagta cgatttaaac attctgccat ttttctctta ctcattcccc
     2161 gtccttcgta cctttgccgg agaatattga ttagcccggg atattcccca agcacctcac
     2221 tgataacgcg atcaataatc aacgcctctg tgtctgtaca atgtgacaac cagctcttct
     2281 gcttccctct ggtcatatcc cgaaaaaatg cctcaagttc cggtttttcc agcccggatt
     2341 tcttcatgct gcgtaaaacc tcattaactg ctgttttcgt cagctttttc gaaaccagta
     2401 accggttaaa catatttccg gatttacccc caccgatata cgaccaccgc ccccacatcc
     2461 gtaatttccc ctggttccag actgcttcca gcgtgttcag gcgtaaatgt tcgccgcttt
     2521 tgcctgtaat ttccggatat atcatattta cgctcactca ctctcaattt tgtaaatctt
     2581 cacacccagc cgtccaccag atactggctg accgcgcaca atattgattt catcaaactg
     2641 ctcgtcatca atgagcactt ccgcatgcgt cagcgcatcc agcggtgctt tcagaatgtt
     2701 gtccaggtca cggcggcgct tatccggtgg ctctgcaatc acctttatcg ccagccttcc
     2761 ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc ggcgataacg
     2821 ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag tgttcaccgt
     2881 cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc caagcacgcc
     2941 agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac catgcttttc
     3001 ttcgaacgcc agcggatccg catgaagctc gttgtgatgc tcccgacaca gcggtagcgt
     3061 gaaaatatcg tgagattttg tccccattcc cccctgacca tgaccaatca ggtgatgcgg
     3121 atcgtcagca ggcttaccac aacacgcaca cggctgtgtc ttcacccagt gcgtgtattt
     3181 ctcgttaacc cagcggcgac gtttaggtcg tttcatgaaa gattccggag actcaggatc
     3241 aacggcaatg ctgaccaccg tcttttcctg tggcggtttt tgctggtgag cgtgaggcag
     3301 cggcgcaaga ttttttgtgc gctgcttcag tatgctggtg gcggtctgct ctcccggtac
     3361 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     3421 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     3481 agccagagat aattcccgtt cctgtgcacc acttattgcg tgacgtatga cgtcaatcat
     3541 ccaggctgtc agattttgtt gagcaagctg cccgagtgat tcggatgtct ggtcgcgcag
     3601 ctggttgtcg cagtgccagc acaacaccat tgcgccggta ccataacggt gaatgacggt
     3661 ttcgctgtga tgataatcgc cgtgtggcca ctggcaggat ttaatatggc gcaacagcca
     3721 gtcagacaat gcaccagcac caccagcagc acgaatcacc cgtgcgttac tgaaaaacgg
     3781 cagcaatgtt ttgtcttcca ctagcggctg gcgaacggca ggaacaaccc cggacggcag
     3841 attacgcatg cttttcggtt ccggctccac cagtacccgg gtattgtgga ataccggcat
     3901 ggattcacga cccggcttaa cgatcaccag cccgagttcc ggtaccagaa caggtcgaag
     3961 taatacccgc acgttacctc cagatgcgtt gctggaatgt gcgggacgga cgcggcgggc
     4021 gttcggaata aaggagcctg acgtagatta tccagtgacg gtagtcgagg ctaagggctt
     4081 tcttaaactc atacccacgt ctgcggtagt tatgaatcag ccattcggcc tgttcttcag
     4141 tgcagggatc gtgctgatac cagtcatatt tgaatgtgtg agaacaccgc ccttgcctgc
     4201 tggcaggggc gatatcagaa ttgtgatgtt tggtattgtg cgccatcggt tttctctgct
     4261 ggcgcagcag gtgccagttg ttcaagctgg cgtgcggcaa tattgtctct gatttctgtt
     4321 gtcgtcaaca ggcaacgtgc tatcatcgaa tggtgttcta tcctactccg tgaggtttac
     4381 catgcgtaca acccaacaat tcagcattac attaactaac gaaatggctg acatggtgcg
     4441 cgcccgtgtg gcttccggtg cctatgcttc agaaagcgag gtcattcgtg aagggcttcg
     4501 cgcactgaat gagcgcgata aagcaatcga agcgtggtta acgcattcag ccgccccctc
     4561 tcttgattct atccgcgaaa atccaaacaa cggacgctcc atttcacagg ttcgcgccgc
     4621 gattcgatcc gggaagtaat ctgcatgaca tatgaagtca tcattactcc tgaggccgaa
     4681 caacaaataa tcaacctgca caggtatata acggagaaag cagggaacgt cattgctgac
     4741 aattatgcca atgcgcttct tgattatctt gatgggtttt ctacattccc gcatcggggc
     4801 aataaacgcg atgatattcg ccaggggatg cgggtaactc atttccgcca cagaacgatt
     4861 attgcttttg ccgttgatag cagaaaagtc tttattgtcg gtatctatca tggtgggcaa
     4921 agttatgaaa ccgatttctt ataaactttt acccacatca ttccggtgtt agaattaacc
     4981 gtccgccccc tctcttactg gcggattcgt aggctatata aatcaaagat cccggctcat
     5041 gtttatgtcg ggatcttttt tcggcgattt atccccagcg gcaaatcgaa tacaccacca
     5101 gcgccaccgc catcgcaatt cctaccgttg tgaatgcttc aggccaggtc atcgtaaaat
     5161 atcctccacg cttatcagtc cgttccgctc cagataactc atcgccttat ccggtaattt
     5221 gcagtctggc ttcgctttcc tcagttgcca ggttaactgc tttaccagca tggttaactc
     5281 atcgaccaga cgctgatatc ccactggttt gtattcatgc aatttaccgg ctggctctgc
     5341 tgccagcgat accagtgcga tttccagaac agcaatatcc atcttatatg tgcggatgat
     5401 gtcatggtcg attgtgcccg gtatgcacag tctctgtgtt tcaatagtct cctctgcgtg
     5461 agctattaac tgctctctgg taaaagtcgt catgccgtag ccccttcttg atatttttca
     5521 aaccagaaca caactgggtc agatttcatt tcaaccaatc ccatacgaac cagcgctttg
     5581 cctttcccgg acgcaaggaa ttcacgacga ccatcactga taattcgccg ataatcttcc
     5641 agactactgc aatgcttgtg cagattgcat gggtggcatg ccggaaccat gttggatata
     5701 tcgtcacgtt cctggtgaag catatttcca tcaaaacgaa tgaccggttt tacatggtct
     5761 gcatgccact tttcgccaag ttcgcagccg caataagcac agcgaccgcc gaacttcatg
     5821 cgcagctctg cacgttgttt tttcgtcagt gccatatcag ctttccttat acggattaat
     5881 tttattgtgc agtgtgctga acgacgccca caccacgtcg ttatacaatt caataactgg
     5941 ctcaattatt ttcacgataa accagaccag taatagcggg ggtatcggta tcatcaacac
     6001 gataaatata atgagaaaca aaaattctgt cgctctactt tttctcggat attcttttct
     6061 gaataatgta gtcatttctt accgcccttt caggcggcct cctgatgttt tgagggtgca
     6121 gaaatccctc cggttaagga ttaaattttt aactaaattt aattattcag gcgcgataag
     6181 ctgttccgca caatccagca atgcttctgt cgcttcctta agcgttactg tatcgctatc
     6241 atccagtcct gcaacttttt cgtacctgac aaacgccgcg caaagctcgt taaacaaccc
     6301 cgcccgtact tcagccagga aagcgtcggt ggccggagtt tcaggaatcc gtctcctcat
     6361 ccgttctatt gcatgattga acccgaaatc ttcagcaaga gatacgtcat ccatattgtc
     6421 attgtcgtcc tcaatatccc gtggttctgg aattgcagac tttattaccg cattctccgc
     6481 cgccagcgcc acaagattag ctttcagctc tgcaattcgt ttgttttggg cttcccgttc
     6541 atccagtagt gccagcgcaa catttggatt aaaagcagca ataaattcag cgtttgcata
     6601 agcctgaaca tctgtttcaa ccaggcagtt aacatgacat tctgcaatta cgccaccggg
     6661 ctctcctttc catttttgac aaacaaaaac tcctgttata ttcccatgct gattgcccga
     6721 tgtatgccct acgatgtagc atcctttagt tgctttctct gctgcttcac gcagcgcctg
     6781 atagtcaatc tcgctcattc atcgccccac tcatcacaat atgcttcgac cggatttttt
     6841 cctgcttcat aatcatcacg ccatgcttca gcatcagcag cactgccacc acgtaactct
     6901 gcatattcca ttaacagttc atgccattct tcaaaactga cgctgtattt agttgaacca
     6961 aaatcagcca ttttgttctt cctcttcgtc ttttatttcg tgatatgaat aattgcagta
     7021 gttaaagaaa atatcttttg cttcgtcatg tatttcatca ggcgtcgcat catcatccac
     7081 ttcgaattca tcctcgaaat ctccaccggc tattcccgtt tcaataatta ttttaaactt
     7141 tcgcatttaa ctaccgccct ttcgggcggc ctcctgacat taatcgttgt ggtaactcat
     7201 ggcttcattt gcaacatcaa ccggatcaac ctcccaccag caataatttg gtgcgtttcc
     7261 ttcaggtgtc cacggttcta attcattttt tgccgcattc t
//
LOCUS       DAZFDP010000205         7230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFDP010000205 DAZFDP010000000
VERSION     DAZFDP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHJ1G_004597"
     CDS             complement(<1..114)
                     /locus_tag="ACHJ1G_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766318.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHJ1G_004598"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHJ1G_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4766319.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHJ1G_004599"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHJ1G_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4766320.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHJ1G_004600"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHJ1G_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO4766321.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHJ1G_004601"
     CDS             complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHJ1G_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4766322.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1692 a         1954 c         1936 g         1648 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcaccgtc ataactctgt cagaaagcat acctaacaga
     6241 caatcttgca cttccagcgg tgtacgcgtt atctcttcga aacggacgat tttgccgtcg
     6301 cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatgggcc atgattgata
     6361 agcagagcgt agttccagcc gtacttgatt tgatcttcag tggtagatgc gccgccctgg
     6421 atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag gagagatttt
     6481 gcggtgccag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata
     6541 cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca
     6601 aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta
     6661 tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc
     6721 agatgattat tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat
     6781 attttacgcg caataaatgt ttggcgcatt agttgtttga gcggaaaata tctggaaagc
     6841 agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag
     6901 gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg
     6961 accccgttgc aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga
     7021 tcgaacctat ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc
     7081 ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
     7141 ggtgctggtg ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa
     7201 ccgtgctcat tgctgtactg gagctattag
//
LOCUS       DAZFDP010000206         7203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFDP010000206 DAZFDP010000000
VERSION     DAZFDP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(313..1379)
                     /locus_tag="ACHJ1G_004602"
                     /pseudo
     CDS             complement(313..1379)
                     /locus_tag="ACHJ1G_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1382..1744)
                     /locus_tag="ACHJ1G_004603"
     CDS             complement(1382..1744)
                     /locus_tag="ACHJ1G_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4766323.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2281..4194
                     /gene="mtlA"
                     /locus_tag="ACHJ1G_004604"
     CDS             2281..4194
                     /gene="mtlA"
                     /locus_tag="ACHJ1G_004604"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4766324.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4424..5572
                     /gene="mtlD"
                     /locus_tag="ACHJ1G_004605"
     CDS             4424..5572
                     /gene="mtlD"
                     /locus_tag="ACHJ1G_004605"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4766325.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5572..6159
                     /gene="mtlR"
                     /locus_tag="ACHJ1G_004606"
     CDS             5572..6159
                     /gene="mtlR"
                     /locus_tag="ACHJ1G_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4766326.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6171..6380)
                     /gene="yibT"
                     /locus_tag="ACHJ1G_004607"
     CDS             complement(6171..6380)
                     /gene="yibT"
                     /locus_tag="ACHJ1G_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4766327.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6665..7027
                     /locus_tag="ACHJ1G_004608"
     CDS             6665..7027
                     /locus_tag="ACHJ1G_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4766328.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1832 a         1808 c         1826 g         1737 t
ORIGIN      
        1 aaatattttt taaatgtgac tgtcatcctt cagataataa agccatcatc gcaatagatg
       61 gaaaagcact cctgctcttt tttgattaga gccgtcgcag gaggaacaac tcatatcatc
      121 attgcgatct cgacgatgta tagtctcatc ctgcggcaga acaagacgga taaaaaatcc
      181 aatgagagca cactcatcca gaacttatta acataatgaa tattaaagaa aaatcatcgc
      241 agctaatgaa gtaatatgca aaaataatta gtccttacaa ggagcaaagt tccataatct
      301 cccccctccc cctcaatgat ccaaataaag ataatgcatc cagctggtca ttcttagcag
      361 cactttccgc atcaccgaaa catggctgaa atggtcggag taaaccgcca cctgggcgta
      421 gatgccgtcg ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac
      481 accgtccgtg ccgggcacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc
      541 gcctggcacg acaggtaaaa tactggtcag tttgccgtgg aacacctgcc caggtagcgc
      601 gttaaacacc acttctgcat catcaccagg tttcagacgt aacagcgagt tttgccgaaa
      661 ttgggcgaca atttgccgtt tttgctcggg gatgaaaacc atcaccggac gcaacggcaa
      721 ggcagctgcg tatgtacctg ggcggatcag tacctgagta atgtagccat tgctcggcgc
      781 gcgaatgaca gtctgctcaa gattatattt tgcttcagta agttgcgctc ttaagctcac
      841 aatctgcgat tgctcgccgt taaccatact gtcgagctgg ctctggatct gcgcctgctc
      901 cgccaccgag cctttcacca gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat
      961 gtcacgttcc gagaacggat tcaccgccgc ctggctgcct ttcagataac gttgataatt
     1021 tttaaacaga cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttctgtgag
     1081 ctgcgcgcgc agagtcttta tattatgcgt cgccgtcatc aggtcagcct gaagcctgtc
     1141 aactcgcgcc tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag
     1201 ctgattattc ttgtcagtga cttcagtaac aattcccgtc acctgtggcg tgatggggat
     1261 cgctatcact gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat
     1321 caaaccactc accagaaaca cgccagcgcc acgtaagtta aaacaatcaa tagatccata
     1381 attagatctc cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca
     1441 gccgggaaat tcggtcagaa agtgcggcga tttccgggtc agtcgctttc tcctgcgacg
     1501 caacatgtga ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca
     1561 gaaacggcca gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt
     1621 cggcatgggg atggttgcgc tttttggcaa tcagataagg gatgtcatgt atcgcgatga
     1681 ttccataaaa aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa
     1741 acatatctgc ctcggattca cgtttatcag tgttgttttt gggctggcag ccagaaggga
     1801 gtcaggctga tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat
     1861 aatttttaga ggtgatgagt tgcttagtta cataacgatt gtatgacgaa ggcataacat
     1921 gctgtagatc acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga
     1981 ttcaacaaac catcaaaaca aaaatgtgac actactcaca tttaaatgcc atttttagcg
     2041 aaaatcgccg ccttgttgct tttttacaca agcgttttgt gatgaacgtc acgtcaatta
     2101 cctctctacc ccctatattt atgtgattga tatcacacaa aaggccgtcg actggacagt
     2161 taaccgattc agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca
     2221 ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
     2281 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg
     2341 atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca
     2401 gggtggttac cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctcctg
     2461 ccgctgctga tcggttatac cggtggtaag ctggtaggcg gcgaacgtgg cggcgtagtc
     2521 ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct
     2581 atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt
     2641 aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
     2701 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg
     2761 ctggctgcgg gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt
     2821 gttgaaccgg cgaaaatcct gttcctcaac aatgccatta accacggtat cttctcgccg
     2881 ctgggtattc agcagtccca tgaactgggt aaatcaatct tcttcctgat tgaagctaac
     2941 ccaggtccag gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg tagcgctaaa
     3001 cagtctgcgg gcggtgcggc aatcatccac ttcctgggtg gtatccacga aatctacttc
     3061 ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc
     3121 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc
     3181 cttgctgtac tggcgatgac accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt
     3241 gcggcgatgg ctgtctcctt cgttgtctct actattttgc tgaaaaccag caaagtgaaa
     3301 gaagaagatg atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa
     3361 ggcacatctc cgctgtctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc
     3421 atcgttgcct gtgacgccgg tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag
     3481 aaaattcagg atgcaggtct gtcacagatt tcggtcacta acagcgcgat caacaacctg
     3541 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag
     3601 gttccgcagg cgcagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc
     3661 ctaaccgaac gtctcgttgc tgcccagcgc catactgaaa acgaagtgaa agtgaaagac
     3721 agtctgaaag acagctttga cgattccagt gctaacctgt tcaaactggg cgcggagaac
     3781 atcttcctcg gtcgcaaagc agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag
     3841 ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa
     3901 ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa
     3961 gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt
     4021 gaagaagaag atgacattgc ccgtctggtg attggtattg cagcccgtaa caacgagcac
     4081 attcaggtta tcaccagcct gaccaatgca ctggatgatg agtccgtcat cgagcgtctg
     4141 gcacacacca ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat
     4201 cccaccctct ccacatggag aaggtggggt taattgcctg atgcgctacg cttatcaggc
     4261 ctacaggatg catcacaatt tgttgaattt gcacgttctt gtaggccgga taaggcgctt
     4321 acgccgcatc cggcgctgcc cctctcctca cggagagggt ttgggtgagg gaaaagcctc
     4381 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4441 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4501 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4561 tcaggttcat gtggttggtg aaaccgagca ggtagatacc gtttccggcg tcaatgctgt
     4621 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc
     4681 cgttggcccg gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg
     4741 caaagaacaa ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg
     4801 taccacgcag ctgaaaggcc atgtgatgaa cgccctgccg gaagacgcca aagcgtgggt
     4861 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc
     4921 ggcaactaac gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa
     4981 aacgcagttc aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat
     5041 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     5101 cggaaaactg gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc
     5161 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga
     5221 cgcagacaaa catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct
     5281 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5341 tctgatcaag ccactgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca
     5401 ggggattgct ggtgcaatgc acttccgcag tgaagatgat ccgcaggctc aggaactggc
     5461 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg gtcttgatgc
     5521 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5581 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5641 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5701 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5761 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5821 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     5881 cgccaggaat acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac
     5941 ggcaacgagt acgcctttac cgacgacgag atccttggac cctttggtga actgcactgc
     6001 gtggcggcgt taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg
     6061 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg
     6121 atttccagaa tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc
     6181 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6241 acgctttcat cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac
     6301 tcccggaacg cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6361 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6421 cgccaatttt agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc
     6481 aataatcatc cgctgctgtt tattatcttt tttctataga tttcttaatt aatcaacgaa
     6541 ttgcgtagtt tttgagcatg ttaatgatta cgttgcagaa ctattacaat gtcatcagtt
     6601 gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta tcagaagcca
     6661 tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc agcgatcgcc tggacgccat
     6721 ccgccaccag caggccgatc tgtcgctggt tgaagccgca gacaaatatg ccgagctgga
     6781 aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa
     6841 actgagcaaa gaagcacaga agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa
     6901 agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc
     6961 aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg actggattct ctaagactgc
     7021 gttttaagcg cctcagacga ctggttcgat catatcaacc cgcatctggc ttgccttagc
     7081 tggtcaatgc ggggactctc ctgtctactc gcctaattac caaacaattc actcccttta
     7141 cgtcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata gccacctata
     7201 aac
//
LOCUS       DAZFDP010000207         7142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFDP010000207 DAZFDP010000000
VERSION     DAZFDP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..706
                     /gene="evgA"
                     /locus_tag="ACHJ1G_004609"
     CDS             92..706
                     /gene="evgA"
                     /locus_tag="ACHJ1G_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4766329.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            711..4304
                     /gene="evgS"
                     /locus_tag="ACHJ1G_004610"
     CDS             711..4304
                     /gene="evgS"
                     /locus_tag="ACHJ1G_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4766330.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4360..5505)
                     /gene="yfdE"
                     /locus_tag="ACHJ1G_004611"
     CDS             complement(4360..5505)
                     /gene="yfdE"
                     /locus_tag="ACHJ1G_004611"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO4766331.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5579..6523)
                     /gene="yfdV"
                     /locus_tag="ACHJ1G_004612"
     CDS             complement(5579..6523)
                     /gene="yfdV"
                     /locus_tag="ACHJ1G_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4766332.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6593..>7142)
                     /locus_tag="ACHJ1G_004613"
     CDS             complement(6593..>7142)
                     /locus_tag="ACHJ1G_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFO4766333.1"
                     /translation="LRENQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIG
                     MGYAIGASVTSGSPVVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVD
                     LSGAGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRKPTIINVVI
                     DPAAGTESGHITKLNPKQVAGN"
BASE COUNT         2192 a         1587 c         1441 g         1922 t
ORIGIN      
        1 tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa
       61 gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc
      121 tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga
      181 gttgactgaa gggggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat
      241 cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg
      301 ccaatatagc ggaattatta ttattgtctc cgctaaaaat gaccattttt acgggaaaca
      361 ttgtgctgat gctggcgcta atggattcgt gagtaaaaaa gaaggcatga acaatatcat
      421 tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt
      481 tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag
      541 tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat
      601 cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag aatgtaaatc
      661 actgatggat ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt
      721 taccctattt ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag
      781 attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc
      841 tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca
      901 agtcccaaac ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg
      961 ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg
     1021 ccgatcatca aaaagcaatg gacgcgcttg cagaaggtga agtcgatata gtgttatcac
     1081 atttagttac ttcgccgcct cttaatagtg acattgctgc aaccaaacca ttgataatta
     1141 cctttccggc gctggtaacc acccttcacg actcaatgcg accgcttacc tcaccaaaac
     1201 cagtaaatat tgctcgggta gcaaattacc ccccagacga ggtaattcat caatcgtttc
     1261 caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc gtatcagccg
     1321 gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt
     1381 tcactcactc cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat aattttttct
     1441 tgaccagaaa agaatccgtc attcttaatg aagtactcaa tagatttgtt gatgctttaa
     1501 caaatgaagt tcgctatgaa gtttcacaaa attggcttga tacaggaaac ctggcctttc
     1561 tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcaa catcccgatt
     1621 taaaggtgct ggaaaatcct tactctcctc cctattctat gacggatgaa aatggctcgg
     1681 ttcggggcgt tatgggggat attcttaata ttattacctt gcaaacaggt ttaaattttt
     1741 ctccgatcac cgtttcacac aatatccatg ccggaacaca gcttaacccc ggtggatggg
     1801 atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttca tttgcagaag
     1861 ctttcataac aacgccatac gtttttgtca tgcaaaaagc acctgacagt gaacaaacat
     1921 taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg caattaaaag
     1981 agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca tttcacaagg
     2041 ttaaggaagg tgaacttgat gctctggtcg cgacacagtt aaattcgcgt tacatgatcg
     2101 atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg aatgcatcgc
     2161 tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga
     2221 atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa atgcccaatg
     2281 tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg acattatccg
     2341 ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc
     2401 gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca ctctcggatt
     2461 ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cataatagtg
     2521 cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca ttagaaaaca
     2581 gtgactcacc ctttaaagat gttttttcta atgcgcatga agtcacagca gaaacgaaag
     2641 aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca
     2701 ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta tatatttgtg
     2761 gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta gaaaaaaata
     2821 aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt cacgaaataa
     2881 gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct ggtcttagca
     2941 aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct
     3001 taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac
     3061 aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg
     3121 caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga
     3181 tcgaccctca ggcgtttaag caggtcttat caaatttact gagcaatgct ctcaaattta
     3241 ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac aaccacgctg
     3301 ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac
     3361 tctttaaacg ctacagccaa acaagtgcag gtcgccagca aacaggatct ggcttaggct
     3421 taatgatctg caaagaatta attaaaaata tgcagggcga tttgtcatta gaaagtcatc
     3481 caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag caagtggcga
     3541 ctgtcgaggc aaaagcagaa caccccatca cactacctga aaagttgagc atattaatcg
     3601 cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg
     3661 atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg
     3721 atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac
     3781 tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg
     3841 aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg ttgaccctgg
     3901 atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc
     3961 gccaccttga tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg
     4021 agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg tttcaagcac
     4081 tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta
     4141 acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgttt
     4201 cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa cacattgcag
     4261 agctggacca ggagattgct gttttctgtc agaaaaatga ctaaatagcg gctcccacaa
     4321 tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg
     4381 aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca
     4441 gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa tcaacatatt
     4501 tctcgcctga gtttgtggca atttaatggc ctcagccaca cttaataacg gcgcgacggg
     4561 tacaccaact tcatgtattc tggctaacca aacttcagct gcctgcgttt ttaacgtccg
     4621 ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg
     4681 gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc
     4741 attaccacaa caaatcgtaa tcggcttatc ctgagtattg aaaacatcaa aaggtgccat
     4801 gtagggatgg cgatttccca gacgttgtgg tgacttccct gtcgcgatat atgccatcag
     4861 accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct
     4921 ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc
     4981 gcatagatcc gcaagagatg taccaacgcg cactggcgga gcatcaggat atcctgtttc
     5041 catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg
     5101 accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgaag
     5161 cgtttcccat gaaaacccca gtttttccat tgtacctggg cgaaaattct cagctaatac
     5221 atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag
     5281 aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc catccacata
     5341 gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc
     5401 gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat
     5461 aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga
     5521 aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt
     5581 atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag cccagtacgc
     5641 tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg gcaataatga
     5701 ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaga tgttcgctgt
     5761 tcaaatgaca tgccatacca acgagaagca gtgccagtgg catcagaatc agcttcagga
     5821 aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg
     5881 cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg
     5941 ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacta
     6001 ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc
     6061 ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag
     6121 aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg
     6181 caaaccccag gaatccaatg gtaggtgaac ccgcaattaa tgcacataca gctgcttctg
     6241 catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa
     6301 taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc
     6361 gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt ttattaaatg
     6421 cgcgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga
     6481 ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa
     6541 gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac
     6601 cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt
     6661 cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac
     6721 gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt
     6781 cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca ctgagatcaa
     6841 caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat
     6901 atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg
     6961 cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata ccgatgccca
     7021 tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc
     7081 gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg ttctcgcgca
     7141 at
//
LOCUS       DAZFDP010000208         7141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFDP010000208 DAZFDP010000000
VERSION     DAZFDP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..1430
                     /gene="xanP"
                     /locus_tag="ACHJ1G_004614"
     CDS             39..1430
                     /gene="xanP"
                     /locus_tag="ACHJ1G_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4766334.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            1551..3260
                     /locus_tag="ACHJ1G_004615"
     CDS             1551..3260
                     /locus_tag="ACHJ1G_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4766335.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3313..5631)
                     /gene="yicI"
                     /locus_tag="ACHJ1G_004616"
     CDS             complement(3313..5631)
                     /gene="yicI"
                     /locus_tag="ACHJ1G_004616"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4766336.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5641..7023)
                     /locus_tag="ACHJ1G_004617"
     CDS             complement(5641..7023)
                     /locus_tag="ACHJ1G_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4766337.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQSSITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
BASE COUNT         1713 a         1915 c         1820 g         1693 t
ORIGIN      
        1 gctaaaatgc ccgctttgct accacgggat tgttttcgat gtctgtttcc accctcgagt
       61 cagaaaatgc gcaaccggtt gcgcagactc aaaacagcga actgatttac cgtcttgaag
      121 atcgtccgcc gcttcctcaa accctgtttg ccgcctgtca gcatctgctg gcgatgttcg
      181 ttgcggtgat cacgccagcg ctattaatct gccaggcgct gggtttaccg gcacaagaca
      241 cgcaacacat tattagtatg tcgctgtttg cctccggtgt ggcatcgatt attcaaatta
      301 aggcctgggg tccggttggc tccgggctgt tgtctattca gggcaccagc ttcaactttg
      361 ttgccccgct gattatgggc ggtaccgcgc tgaaaaccgg tggtgctgat gttcctacca
      421 tgatggcggc tttgttcggc acgttgatgc tggcaagttg caccgagatg gtgatctccc
      481 gcgttctgca tctggcgcgc cgcattatta cgccgctggt ttctggcgtt gtggtgatga
      541 ttatcggcct gtcgctaatt caggttgggt taacgtccat tggcggcggt tacgcagcca
      601 tgagcgataa caccttcggc gcaccgaaaa atctgctgct ggcaggcgtg gtcttagcct
      661 taattatcct gcttaaccgt caacgtaacc cttacttacg cgtggcctca ctggtgattg
      721 cgatggcggc cggatatgcg ctggcgtggt ttatgggcat gttgccagaa agcaacgaac
      781 cgatgacgca agaactgatt atggtgccaa cgccgctcta ttacggtctt ggcattgaat
      841 ggagtctgct gctgccgctg atgctggtct ttatgatcac ttcgctggaa accattggcg
      901 atatcacggc gacctctgac gtttccgaac agccagtgtc cggtccgctg tacatgaaac
      961 gcctgaaagg cggcgtgctg gcaaacggcc tgaactcgtt tgtttcggcg gtgtttaaca
     1021 ccttcccgaa ctcctgcttc gggcaaaaca acggagtgat ccagttgact ggtgttgcca
     1081 gccgctatgt cggttttgtc gtcgcgctga tgttgatcgt gctgggtctg ttcccggcag
     1141 tgagcggttt tgtacaacac attccagaac cggttctggg cggcgcaacg cttgtaatgt
     1201 ttggcactat cgccgcctcc ggtgtgcgta tcgtttctcg tgaaccgctg aaccgtcggg
     1261 cgattctgat tatcgcgctg tcgctggcgg ttggtctggg cgtgtctcag cagccgctga
     1321 ttttgcagtt tgcccctgaa tggctgaaaa acctgctctc ctccgggatc gccgcgggcg
     1381 gtattactgc catcgtgctg aatctgattt tcccaccaga aaaacagtaa ttcactggcg
     1441 gcggtaagat aattaccgcc gcagtctcac ctctttcacc attcccgcct tgaggattgc
     1501 gcgtaaatcg tgcataactc ccttatgtgc atttcacggg atggaagacc atgaaattta
     1561 ttgggaagct gcttctctac attctcatcg ctctgttagt ggtgatcgct ggcctctatt
     1621 ttcttctgca aacccgctgg ggagcagaac atatcagcgc atgggtttcc gagaatagcg
     1681 actatcatct ggccttcggg gcgatggatc accgtttttc cgcgccatct catatcgtgc
     1741 tggagaacgt cacgtttggc cgtgatggcc agcccgcgac cctggtggca aaaagtgtcg
     1801 acattgcgct aagcagtcgg caactgaccg aaccacgcca tgtcgatacc atcctgctgg
     1861 aaaacgggac gctgaatctc accgaccaga ccgcgccgct accgttcaaa gccgatcgtc
     1921 tgcaactgcg tgatatggcg tttaatagcc cgaatagcga atggaaactg agcgcgcagc
     1981 ggataaatgg cggcgtagtt ccgtggtcac caaaagccgg taaagtgctg ggtacgaagg
     2041 cgcagattca gtttagtgcc ggatcgcttt cgctcaatga tgttcctgcc accaatgtac
     2101 tgattgaagg cagtattgat aacgatcgcg ttacgctgac taacctgggt gccgacatcg
     2161 cccgcgggac attaaccgga aacgcgcagc gtaacgccga cggcagctgg caagtggaaa
     2221 acctgcgcat ggcggatata cgtctacaaa gcgaaaaatc gctaaccgac ttctttgcgc
     2281 cattacgctc tgtcccgtcg ttgcagattg gtcgcctgga agtgatcgat gctcgtttgc
     2341 aaggtccgga ctgggcggtg accgacctcg atctcagctt gcgcaacatg accttcagta
     2401 aagatgactg gcagacacag gaaggcaaac tgtcgatgaa cgctagcgag ttcatttatg
     2461 gttcgctgca tttatttgac ccgattataa acgcggaatt ttccccgcag ggcgtagcgc
     2521 tgcgccagtt caccagccgc tgggaagggg gtatggtcag aacgtcaggg aactggctgc
     2581 gtgacgggaa aacgttgatc cttgatgatg tggcaattgc cgggctggaa tataccttgc
     2641 cgaaaaactg gcaacagttg tggatggaaa cgacacccgg ttggttaaac agcctgcaac
     2701 tgaagagatt tagcgccagc cgcaatctga tcattgatat cgaccctgac ttcccgtggc
     2761 agctcaccgc gctcgatggt tacggtgcca acctgacgct ggttaccgat cataaatggg
     2821 gcgtctggag tggctcggcg aatctgaatg ccgccgccgc gacattcaat cgtgttgatg
     2881 ttcgtcgccc gtcgctggcg ctgaccgcca acagcagcac ggtgaatatc agcgaactga
     2941 gtgcatttac tgaaaaaggc attctggaag ccactgccag tgtttcacaa acgccacaac
     3001 gtcagaccca tatcagcctg aatggacgcg gtgtgccggt gaatattttg caacaatggg
     3061 gatggcctga attaccgttg actggcgacg gcaatattca gcttaccgcc agtggagata
     3121 ttcaggccaa tgccccgctg aaacctacgg ttagcgggca attgcatgcc gtgaacgccg
     3181 caaagcagca agtgactcaa accatgaatg ctggcatcgt ttccagcggt gaagttacgt
     3241 cggcagagct ggtgcagtaa acatcaatct cgccgggtac gactcagacg tacccggcat
     3301 tccatcaata gattacaacg taattgtcag cgcattccct tgaggcttca ccaccagccc
     3361 ctgctcactt tcagcctgcg aaccgtcttg cagaccattt actttcacaa cattgcgcag
     3421 gcacagtgtc cagttcttcg cctcgcccgc accagtcaca gtaatcgtgt tgccagtacg
     3481 tgctgctttt aaagtaaaga tcaccgatcc gtcagcagcg ggcacttcac agacggcttc
     3541 atgcccgtct tgcagattaa agaggtggaa tgccgtgcct tcgtgccaca cgtaatcggg
     3601 acgttgatcg ttgttgccca gcgccagtag agtgttatca cgcacataaa cgggcagact
     3661 caggaagccg tgctgctgtt tatgccagcg actaccgtcg agttcatcgt tgtgccacag
     3721 gtgtgtccag cgaccttccg gcaggtagaa ctgcacatcg cccgcttcag tgaacaccgg
     3781 cgcaaccatc acgttgttgc ctaacatgta ttgacggtca aggtaatcac aagccggatc
     3841 gtccgggaac tccatcatca tggcccgcat catcggcgta ccccgcgcgt tcgcacgcgc
     3901 agcttcacga tacagatacg gcatcatgcg gcatttcagt tgcgtgaaga agcgcactac
     3961 atcacaggac tcatcatcgt aggcccacgg cacacgataa gatttgctac cgtgtaaacg
     4021 gctatggctg gagagcaaac caaacgcgca ccagcgtttg taaacgtgcg ccggagcggt
     4081 attttcaaag ccgccgatat cgtggctcca gaagccaaaa cctgaaaggc caatagacaa
     4141 accaccgcgc aggctttccg ccattgattc gtagttagcg taacaatcgc caccccagtg
     4201 taccgggaat ttctgcgcac cgacggaggc cgagcgggca aacaagacag cttcttcctc
     4261 accaacggtg tccttgagca cgttccacac cagttcgttg tagatgtatg cataatggtt
     4321 atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca tcagttggga tacgttcgcc
     4381 aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga cctttcagtt tgtcggcgta
     4441 ccatttgcag gcatccggat tggtaaagtc ataaatcgcc agacctggct gccatttatc
     4501 ccactgccat agcgaaccgt ccgggcgttt gagtaaatag cctttctctt gtaactcttt
     4561 aaagacgggg gatttttgac cgatataggg gttaatccag acgcagattt tcagcccttt
     4621 cgctttcagg cggcggatca tcccttccgg gtcagggaaa gtcagcgggt cccactcaaa
     4681 atcgcaccac tggaaggctt tcatccagaa acagtcaaag tggaaaacat gcagcggcag
     4741 attgcgttcc gccataccat cgataaagct gtttaccgtc gcttcgtcgt agttggtggt
     4801 aaatgaagtg gttagccaca ggccgaagga ccacgcgggc ggcagcgccg gacgaccagt
     4861 aaagcgggta taacgatcaa gtaccgcttt cggcgtcggg ccgtcgataa caaagtattc
     4921 gagatattca ctctcaacgc tgaactgcac tttggagact ttctccgatc ccacttcaaa
     4981 agagacacac tggggatgat tgaccagtac cccataacca cggttagtca tgtagaacgg
     5041 gatattttta tacgcctgtt cagtacttgt gccgccgtcc cggttccagg tctctaccgt
     5101 ctggccattg cgcaccaggg cagtaaagcg ctctcccaga ccgtaaactg tttcgccaac
     5161 gccaagatca agccgctcaa acatataatt gcgttgatta ttcgtgtcct gcacgtagcc
     5221 attatttttc acctgactac cggtaatacg ttcgccgttg cgcagaaaat ccagtgacca
     5281 gaactcacct ttgctgacac gcgcgcttaa gttgccactt ttaaactcag cataacgttc
     5341 tgtgttttcg attgtgacct tcacgtcctg caaaatattg agcggataat gaggaccgtt
     5401 attcagcgcc ccctgaaaat gctcaatccg cacaccgaca ataccttcct gtggggagaa
     5461 aaagcgcagc gtaaataaag gcgtatcaag ctgccaggta cgttcacgca catcacgggg
     5521 ggcagcatag accaccattt cattaccctg ctgttcaacc tcgaatacct gaagcgggtg
     5581 aatcaaattg aggccaggtt gaatcaacca gtttccatcg ctaattttca ttgccgttcc
     5641 ttagttctgc acttcttgag aggtgaattg ttgctggcaa cggcgtttac cctgagccag
     5701 ctgttccata acggttttca gattgtgcgt cgtgagtgag tagtagcgtt tagcgataat
     5761 cgcgctcagc aaataacaga tcgccggaac aatcgtgaat agcgcaataa tgatgctaat
     5821 cgtggcgctg ttctgcgctt tttctgccgc atcatatccg ccataagcca gcatccagcc
     5881 gataagagcg ccgccgaagg ccaaccccag tttgagcaca aacagcgtgc cagcaaaact
     5941 gatcccggtc aggcgtttac cattgcacca ctcgccgtag tcgacggtat cggacatcat
     6001 tacccactgg ataggtgtca ccagttgatg caacacacca atcacgaaga tgaagacaaa
     6061 catagtgatg ctggactgca tgggaacaaa gaacatcgcg aggctaatca ctgccagcag
     6121 ggcgttcgtc caccagaaga tagtgacttt acatttccag tcggtcagag gttttgccag
     6181 tgcggaacca atcaggttac ccacgcaata agtggtgaga aaagcgacaa acacttccgg
     6241 cgtgcccaaa atccatgtga cgtaatacat catcgcccca ccgcgtacgc acaccgccag
     6301 gatattgaaa atggttagta aaccgacaat ccgccactgg tcgttttgcc agatatcacg
     6361 taaatcttcc cgcatagacg ttgttgtagg tggtgcttca acgcgttctt tagtggtgaa
     6421 gaaacagaat gccagcatca tgaatgccac cacggaaagg accgcgatac cgccctggaa
     6481 accgagtggt ttattatcac cgccaattaa attaaccagt ggcatcatca gaacagtaga
     6541 aagcatgcct cccgcggtcg ccagcacaaa acgccaggat tgcagcgaga tacgctgagt
     6601 cgggtcattg gtgattacac cacccaatgc gcagtaaggg atattgacga cggtataaag
     6661 taaggtaagt agggtgtaag taattgctgc atagatcatt ttgccgttca tactgagatc
     6721 tggcgtgcta taggccagta cacagacgat cccgaatggc agtgcgccaa acagtaccca
     6781 cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc aacaacccca tgcaaggatc
     6841 ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt ccgacaaaac cggcaggaat
     6901 gccaaaaata tcggtataaa agaacatcat atataacatt acgttatcga aaataatgtg
     6961 gctggcggcg tctcccatgc cataaccaat tttctcttta acggacaaca cttcactctt
     7021 catctgcttt tccttgatgc tataggggtg ctgagaccga taacgcattt tgtaaaccat
     7081 ccctgtgaga gcgaatattc cgatatctgg ttattaattt atgattcttg ttttatgtga
     7141 t
//
LOCUS       DAZFDP010000209         7085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFDP010000209 DAZFDP010000000
VERSION     DAZFDP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(187..1380)
                     /gene="tyrB"
                     /locus_tag="ACHJ1G_004618"
     CDS             complement(187..1380)
                     /gene="tyrB"
                     /locus_tag="ACHJ1G_004618"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4766338.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1615..2712)
                     /gene="alr"
                     /locus_tag="ACHJ1G_004619"
     CDS             complement(1615..2712)
                     /gene="alr"
                     /locus_tag="ACHJ1G_004619"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4766339.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2765..4180)
                     /gene="dnaB"
                     /locus_tag="ACHJ1G_004620"
     CDS             complement(2765..4180)
                     /gene="dnaB"
                     /locus_tag="ACHJ1G_004620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4766340.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4263..5246
                     /locus_tag="ACHJ1G_004621"
     CDS             4263..5246
                     /locus_tag="ACHJ1G_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4766341.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5412..5654)
                     /gene="pspG"
                     /locus_tag="ACHJ1G_004622"
     CDS             complement(5412..5654)
                     /gene="pspG"
                     /locus_tag="ACHJ1G_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4766342.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5788..6825)
                     /gene="dusA"
                     /locus_tag="ACHJ1G_004623"
     CDS             complement(5788..6825)
                     /gene="dusA"
                     /locus_tag="ACHJ1G_004623"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4766343.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1788 a         1884 c         1757 g         1656 t
ORIGIN      
        1 acacccaaaa aaacaggata aggaattcca gataattggg ataagcttaa caataaaaga
       61 actaacaacg tttattatct ctcacgtaga acgatggcat caaaaaaatc atctataaag
      121 aaagagtgaa agcgacgaca gtttaatttc actgcaggct gggtagctcc agcctgcttt
      181 cctgcattac atcaccgcag caaacgcctt tgccacacgt tgtacatttg ccgtatttaa
      241 cccggcgaca cacatgcgac cgctggcgat gagatagaca ccaaattctt cacgtagtcg
      301 gtcaacctga gcggcactta aaccggtata actgaacatg ccgcgctgat taagcagata
      361 atcgaaattg cgttctggca tctctgtgct taataccttc accagttcct gacgcattgc
      421 cagaatgcga gtacgcatct cttctacttc cgccagccag ctggctttca atgcctcgtc
      481 attcagcact gcagccacca cctgcgcacc aaaattcggc gggctggagt agttgcggcg
      541 aactgttgct ttcaattgcc ccagtacgcg gccagcggct tcggcatctt cacacagaac
      601 agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt ttcgagaacg aattgctcac
      661 cagagcgggt aatccagcgc tggcaatggc gcgaatagcg taggcatcct cttccatacc
      721 ggcaccaaat ccttgatagg caatatcgag gaatggaata agctcgcggg atttgagaat
      781 ttcaatcacc gcatcccact gatcattagt gagatcggca cccgttgggt tgtggcaaca
      841 tggatgcagc aacacaatac tgcgggcagg taatgttttc agcgtcgcca acaggtcatt
      901 aaagcgcacg ccgttagtcg cttcgtcata ccaggggtaa gtactcactt cgaatccagc
      961 cccggcgaat attgctacgt ggttttccca ggtaggatcg ctgacccaga cgcctgattc
     1021 cgggaagtag cgtttcagga aatccgcgcc cactttcaat gcccctgagc cgccaagggt
     1081 ttgaatggtt gctacgcgct gttgttgcag tactggatgg tccgcaccaa acagcagcgg
     1141 cgcaatggca tggcgatagc tgttaagccc ttccatcggt aaataaagcg aagcgccatg
     1201 aggctgcgca ttcaggcgcg cttccgcctc cgccacggct tgcagttgtg gaataattcc
     1261 gtcttcgttg tagtacagac cgatacttaa attcactttg tcgctgcgag ggtcttcttt
     1321 aaaacgctcc ataagcgtaa gaatcgggtc gccagcgtag gcgtcaactt tttgaaacac
     1381 gcgatggttc tccaggttta cgggcaggtg gttaaaacac aataaaccgg aagaaggcga
     1441 agatcgagtg gatgttcagg agcgaacggc aattagcaac agagtgagac tcatgacaaa
     1501 cgtacatccg ccagaggcac gacctttata agagcatgaa agaacatcaa cttattgaat
     1561 ttttaggatt ttattggccg gataaggcat tcacgccgca tccggcacag acaatcaaat
     1621 attacagaac gataaatcca cgtatttcat cgcgaccctt gaagtcaggc gcgtaataag
     1681 ttcgtaagcg cttactttcg tcatttcagc gatacgttct acgggcaaac cttcgcccca
     1741 taaaatgacc ggatccccgg ctttgtcctg cgcttgtgga cctaagtcta cgcagatcat
     1801 atccatcgcc acgcgcccga caatcggtac ttcgcgaccg ttcaccagca ctggcgtacc
     1861 ggacggcgcg gcgcgcggat aaccatcgcc atagcccatc gcgactacgc caagacgggt
     1921 atcacgttcg cttacccagg ttccaccata accaacaggt tctccggctt tatgctcacg
     1981 cacggcaatc aggctggagg ttagtgacat cactggctga cagccaaaat cggcaccggt
     2041 ggagcgatct tccagcggcg agacgccata aagaatgatg cccgggcgca cccagtcaaa
     2101 atgcgactgt ggccacagca gaatgccacc cgatgcggca atggaacgtt gaccaggttt
     2161 gccttcgcaa aaggtattaa agatagcgag ttgtttctcg gttgcgccac attttggttc
     2221 atccgcgcgc gcaaaatggc tgacgatatt caccggctga cgaacgtttt tgcactgggt
     2281 caggcgatga taaaacgcct cagcctgttc cggccttacg cccagacggt gcataccggt
     2341 atcgagtttc atccagacgg taaccggctc gtccaggcta gcctcttcca gcgcagccag
     2401 ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca gaaatcgtcg gcagatctct
     2461 ggcatcaaaa aagccttcga gtaacagtac aggtttggtg attccccccg cacgcagtcg
     2521 cagagcttct tcgagacggg ctacgccaaa ggcgtcagca tcggggagcg ttcgcgcggt
     2581 ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc gcaaccattt tactggcagg
     2641 ggccagttca cgaagacgtt gcaggttgtg tcgcagagcg cggcggttaa tcacaacagt
     2701 tgccgcttgc atttgtgttc cttgataagt gtttgcttta attacctaat tcataaaata
     2761 attattattc gtcgtcgtac tgcggccccg catagttgtc gaagcgcgac cattgaccgt
     2821 taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg tttaccgata ataatttccg
     2881 cgatgccttt taaatcactg ttttcgtgat acacctcatc acgatagata aacatgatca
     2941 agtccgcatc ctgctcgata gagccagatt cacgcaggtc ggagttgacc gggcgtttgt
     3001 cggcacgttg ttccagagaa cggttcaact gggacagcgc caccaccggc acgttcagtt
     3061 ctttcgccag tgctttcagc gagcgggaga tttctgcaat ttccagcgta cggttatcgg
     3121 aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat gataagcccg atgccgccgt
     3181 gttcacgggc aatacggcgt gcgcgggaac gcacttccgt tggcgtcagg ccggaggagt
     3241 catcgatata gatattgcgt ttttcgagca aaatacccat ggtgccggaa atgcgcgccc
     3301 agtcttcatc atcgagctgc ccggtacgga ttttagtctg gtcaacgcgc gacagcgacg
     3361 ccagagaacg catcataatc tgttctgaag gcatctccag cgagaagata agtactggtt
     3421 tatcctgcaa catcgccgcg ttttcgacga ggttcatcgc aaatgttgtt ttacccatcg
     3481 acggacgcgc ggcgacgatg atcaaatccg acggctgcaa gccagcggtt tttttgttga
     3541 gatcgtcata accggtgttt actccggtaa cgccatcgtg tggctgctga aacaactgct
     3601 caatacgcgc cacggttgcg tcgagcacat cggcgatgtt cttcggccct tcgtctttgt
     3661 ttgcacgact ttcggcaatt ttaaagacgc gggattcagc aaggtccagc agatcttcgc
     3721 tggtacgccc ctgcggatca aaaccggctt cagcaatctc attcgcaacc gagatcatct
     3781 cacggacaac ggcacgttca cgcacgatgt cagcataagc actgatgttc gccgcacttg
     3841 gcgtattttt tgacagctct gccagataag caaaaccacc gacgctatcg agttgcccct
     3901 ggcgttccag cgattccgca agggtaatca gatcgatagg actaccgctt tcctgcaaac
     3961 gcgccatttc agtaaagata tggcggtgtg ggcgggtgta aaaatcgtct gccaccacac
     4021 gctcggctac atcgtcccag cgttcgttat ccagcattaa accgcccaac accgactgct
     4081 ccgcttcgat cgagtgcgga ggcactttca gcccggcaac ttgtggatcg cgttcgcggg
     4141 gttcagcctg ctgtttgttg aagggtttat ttcctgccat agtgaatgga gttaccgaga
     4201 taaagaatgg gtcgaaactt taccatatga agcagcccct gacgatacgt tctggaggac
     4261 acatggcaac acgaattgaa tttcacaagc acggtggccc ggaagtactt caagccgtag
     4321 agttcactcc tgccgatccg gcagagaatg aaatccaggt cgaaaataaa gccatcggca
     4381 tcaattttat cgacacgtat atccgcagcg gcctttaccc gccgccatca ctacccagcg
     4441 gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg cagtggtgta aagcatatta
     4501 aggcaggcga tcgtgttgtc tatgcgcagt cggcgttagg cgcttacagc tctgtgcata
     4561 acattaatgc ggataaagcg gcgattctgc ctgcggcaat ttcttttgag caagctgcgg
     4621 catccttcct gaaaggctta acggtttatt atctgctgcg taaaacctat gaaattaaac
     4681 ccgatgaaca gttcctgttc cacgcagcgg ctggcggcgt tggcttgatt gcctgccagt
     4741 gggcaaaagc cctgggcgcg aaacttatcg gcaccgtagg aaccgcgcaa aaagcgcaga
     4801 gcgcgctaaa agcgggcgcg tggcaggtaa ttaactatcg tgaagagaat ctggtcgagc
     4861 ggttaaaaga gatcaccggc ggtaagaaag tgcgcgtggt gtacgattcc gtgggcagag
     4921 acacctggga acggtcgctg gattgcctgc aacgccgcgg cttaatggtc agttttggca
     4981 actcatcagg tgcggttacc ggtgtgaact taggcattct caatcaaaaa ggctcgttgt
     5041 atgtgacacg cccttccctg caaggctata tcaccacgcg ggaggaatta accgaggcca
     5101 gtaatgaact gttctctttg attgccagcg gtgtgattaa ggtcgatgtc gccgagcagc
     5161 agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat tctggaaagc cgggcgacgc
     5221 aaggttccag cctgctgatt ccataaaaga aatagggctt ccacctggga agccctttct
     5281 ttttatagtt cggctgtatg tagggtacag cacgatgaat ctgttagagg cgcaatagtg
     5341 acagatttga ttatcaattc ctattttgtt ctaaggataa aaccttaggt tgtgatcatc
     5401 cgcacaatcc cttagtaacg ccagcggtca taacgctgat atttaggcac ttttggtgct
     5461 ttaatcgcct taataaccca caccaccgca atcgccagta gtaaccacgg cagcaactta
     5521 atcatcaatg ccagcatacc gccgaggaac ataatggccg tcgccacaac cagcgcggcg
     5581 ataatgccca gcaacgaaac gccggtgacc atcagcatga caaaaaagcc aatcacaaaa
     5641 agtagttcca gcatgatgct ctcccaaata tgaaatctct tgctggcatt acaagaatca
     5701 tgccaaaaat aatctattga tttaacagca aaacgccccg cgacggtgcg cagggcgtgg
     5761 tgaatttgac tactttttgg tgaaaagtta acgcttatcc gccaccagtt tgagcgcgtg
     5821 ttccagcaca ttaatgtctg cacccgcttt atgggcattt tcacttaaat aacgccgcca
     5881 ctgccgcgcg ccaggaatac cctggaacaa gcccaacata tgccgggtaa tatggccgag
     5941 atacgtcccc tggctgagtt cacgctcaat gtacggatac atggcgcgca ctaccgccac
     6001 cggatcggca tcggtatccg aggaaccaaa gatctcccgg tctaccgccg ccagaatacc
     6061 cggattctga tacgcctcgc gcccgaccat cacgccatcc atatgttgca ggtgtgcttt
     6121 ggcctcttcc agcgacttga taccaccgtt aatcgacatt gtcagatgcg gaaagtcacg
     6181 cttcagttga tacacacgcg gataatcgag cggcgggatt tcacggtttt ctttcggact
     6241 taaccccgaa agccaggctt tacgtgcgtg gatgataaac atctcacact cgcctttgcc
     6301 ggaaacggtg ttgatgaaat cgcagagaaa ttcatagctg tcctggtcat cgatgccaat
     6361 acgcgttttc accgtcactg gaatcgacac cacatcgcgc atcgctttca cgcagtcggc
     6421 aaccagctgc gtattaccca tcagacacgc accaaacatg ccgttctgca cccggtcaga
     6481 cgggcagccg acattcaggt tgatctcatc atatccacgc gcttctgcca gcttcgcaca
     6541 ctgtgccagc gccgccggat cgctaccgcc gagttgcaac gctaccggat gttcttcttc
     6601 actgtacgcc aggtaatcac ctttaccgtg aataatcgcc cctgtggtca ccatttcggt
     6661 atacagcaac gtattgcggg aaagcagacg caagaaatag cggcaatgtc tgtccgtcca
     6721 gtcgagcatt ggtgcaacgc taaaccgacc actccagtga acgtcagttt tttcaggcat
     6781 tgcgctggtt tggttgattt tttgcatttc agaattaccg tgcattttca aatgtagaga
     6841 ttattttatc gatatatcat ggggttatgt tattcagcat cactattcag gaggctcaat
     6901 agcggggtac tataccataa caacaggaag cgcctgtctc attgcaaaag aaaattgaga
     6961 tcctctcaag gcatgaagct ctcacgaagt gatggaaata atcttattag ccgttaactt
     7021 tgttaaggcc aatgataaac aatccaggtt cgacgataaa taaaaaatca cacattaaac
     7081 tctgg
//
LOCUS       DAZFDP010000210         7061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFDP010000210 DAZFDP010000000
VERSION     DAZFDP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            176..787
                     /gene="ruvA"
                     /locus_tag="ACHJ1G_004624"
     CDS             176..787
                     /gene="ruvA"
                     /locus_tag="ACHJ1G_004624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4766344.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            796..1806
                     /gene="ruvB"
                     /locus_tag="ACHJ1G_004625"
     CDS             796..1806
                     /gene="ruvB"
                     /locus_tag="ACHJ1G_004625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4766345.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1953..2738)
                     /gene="znuB"
                     /locus_tag="ACHJ1G_004626"
     CDS             complement(1953..2738)
                     /gene="znuB"
                     /locus_tag="ACHJ1G_004626"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4766346.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2735..3490)
                     /gene="znuC"
                     /locus_tag="ACHJ1G_004627"
     CDS             complement(2735..3490)
                     /gene="znuC"
                     /locus_tag="ACHJ1G_004627"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4766347.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3569..4501
                     /gene="znuA"
                     /locus_tag="ACHJ1G_004628"
     CDS             3569..4501
                     /gene="znuA"
                     /locus_tag="ACHJ1G_004628"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4766348.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4517..5839
                     /gene="mepM"
                     /locus_tag="ACHJ1G_004629"
     CDS             4517..5839
                     /gene="mepM"
                     /locus_tag="ACHJ1G_004629"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4766349.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQVVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5959..6930
                     /gene="lpxM"
                     /locus_tag="ACHJ1G_004630"
                     /gene_synonym="msbB"
     CDS             5959..6930
                     /gene="lpxM"
                     /locus_tag="ACHJ1G_004630"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4766350.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1842 a         1727 c         1834 g         1658 t
ORIGIN      
        1 tgaatatgta atattcaaat atttgcttcc aatataacct gtagaataaa ttatactgcg
       61 ccatttttca gatcgttggg acgcctcgca agttttcttc atccttcgct ggatatctat
      121 ccagcatttt tttatcatac agcattatct ttgattcatt acgcaggagc gtcatgtgat
      181 aggcagactc agaggcatca tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg
      241 cggcgtaggc tatgaagtgc atatgccgat gacctgtttt tatgaactcc ctgaagcggg
      301 tcaggaagcg atcgttttca cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg
      361 ttttaacaat aaacaagagc gcacattgtt caaagagttg atcaaaacca acggcgtcgg
      421 cccgaagttg gcgctggcga tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt
      481 tgagcgtgaa gaagtggggg cactggtgaa actgccgggt attggcaaaa aaaccgccga
      541 acgcttgatt gttgaaatga aagaccgatt taaaggtttg catggcgatc tctttacgcc
      601 agccgccgac ctggtactca cgtcacctgc cagcccgacg accgacgatg ctgaacagga
      661 agcggttgcc gcgctggtgg cgctgggcta taaaccacaa gaagcaagcc gcatggtgag
      721 caaaatcgct cgccctgacg ccagcagtga aacattaatt cgcgaagccc tacgcgccgc
      781 gttatgaggt aaaggatgat tgaagcagac cgtctgattt ctgccggtac cactttgccg
      841 gaagatgtgg cagatcgcgc cattcgccct aaattactgg aagagtatgt tggtcagccg
      901 caggttcgtt cacagatgga gattttcatc aaagcagcga aactgcgcgg cgatgccctc
      961 gatcatctgt tgattttcgg tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc
     1021 gccaatgaaa tgggcgttaa tttacgcacg acttctggtc cggtgctgga aaaggcgggc
     1081 gatctggcag cgatgctcac taaccttgaa ccgcatgacg tgttgtttat tgatgagatc
     1141 caccgtcttt cgccagtggt ggaagaggtg ttgtatccgg caatggaaga ctaccaactg
     1201 gatatcatga ttggtgaagg tccggcggca cgctccatta aaattgattt gccgccgttt
     1261 accctgattg gtgcaaccac gcgcgcaggt tcgctgacat caccgttgcg cgatcgtttt
     1321 ggtattgtgc agcgtctgga gttttatcag gtgccggatc tgcaatatat cgtcagtcgc
     1381 agcgcacgct ttatggggct tgagatgagt gatgacggcg cgctggaagt tgctcgtcga
     1441 gctcgcggta cgccgcgcat tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa
     1501 gtgaagcacg atggcaccat ctcggcagat atcgctgctc aggcgttgga tatgttgaat
     1561 gtcgatgctg aaggtttcga ttatatggac cgcaagttat tgctggcggt aatcgataag
     1621 ttctttggtg ggccggtagg tctggataac cttgcggcag ccattggcga agaacgtgaa
     1681 accattgagg atgtgctgga accttatttg attcagcaag gctttttgca gcgtacaccg
     1741 cgtgggcgta tggcgacgac gcgggcgtgg aatcactttg gcataacgcc gccagaaatg
     1801 ccgtaagtcg gattggtcgc tcgcgccgca tccgacactg gcagtgtgtc tgatgcgaca
     1861 ctgttcgcgt cttatcaggc cagccagaga tagcgaaccg taggtcggat aaggcgctcg
     1921 cgccgcatcc gacaaatgtg ttcagcgata gattagctgg cctgcttttt catcatactg
     1981 agaataaata acagtgccgc acatagcacc accgacggac ccgccggcgt atcgtaaaac
     2041 gcggaaaagg ttaaaccgcc agttactgcc accatcccca ccaaaacagc gacaccagcc
     2101 atctgttccg gcgtacgggc aaagcgacgc gcagtagctg caggaataat cagcagtgaa
     2161 gtaataatca acgcgccaac aaatttcatc gctacaccaa tcgtcaatgc cgtcaccagc
     2221 atcaacaaca atttcacgcg ctgtaatttc acaccatcaa caaacgccag atccgggcta
     2281 atcgtcatcg acagcaaatt gcgccattgc cagaacaaaa tagccaccac gatgaccacg
     2341 ccaatcgcaa tagcgatgag atcttctggc gtcactgcca gcaaatcacc gaacaggtaa
     2401 gccatcaaat caacacgaat gttagacatc agactaacga ccaccaggcc cagcgacagg
     2461 gcactgtgcg ccataatccc taataacgtg tcaatcgcca gctgtggacg cttctccagc
     2521 cataccagac cgcccgccag gagcagcgta acggcaatta ccgcatagaa tggattcacg
     2581 tccagcaaca aaccaaacgc gacgccaagt aatgaggcat gagccagcgt atcaccgaaa
     2641 taagacatac gacgccagac tacaaacgaa cccagcggac ccgcggcaca ggcgagcatg
     2701 atcccggcta accaaccggg aaataataat tcaatcatga gcgatcattt ccccgacgca
     2761 aaacaattcg tccctgtaaa tcgtgacgat gattatgatg atggcgatag atacccagtt
     2821 gttcagcacc acgaggacca aacatagaaa taaactccgg atgtagggaa acaacttccg
     2881 gtgtgccgga acaacaaatg tggtgattca gacacagcac ttcatcggtt tttgccatta
     2941 ccagatgcag atcgtgagag accattaaaa cgccacaatc cagttcgcga cgcaactggt
     3001 caataaggtc atataacgcc acctggccat ttacatccac gccttgagtg ggttcatcca
     3061 gcactaataa ttgcggtcga tttaacaatg ctcgcgctaa cagtacacgc tgtgtttcgc
     3121 caccagagag cttttgcatc ggtgcgttaa tcagatgccc ggcctggaca cgtttcagtg
     3181 caggcaaaat atcttcttta tgtgtgccag ggcgtaagcg taaaaaacgg tttacggtca
     3241 gtggcaacgt ggtgtcgaga tacagcttct gcggtacata gccgatgcgt agttttccgt
     3301 tgcgcttgat aaccccttca tcgggtgtta ccagcccgag cactacccgt accagtgtcg
     3361 acttacctgc gccatttggc ccaagtaaag tcaaaatttt tccaggttta agttccagcg
     3421 acacatcaga gaggacgcgg cgttggccaa aagaaaccga gacattttcc agggaaacca
     3481 gacttgtcat gttaatttta gtcttgcagt agtcatgaaa tgttataata tcacacttct
     3541 catattcatt acgatgattg gtcgcattat gttacataaa aaaacgcttc ttttcgcagc
     3601 attatccgcc gctctctggg gaggtgcaac acaggccgca gatgccgccg ttgtcgcttc
     3661 gcttaaaccc gttgggttca tcgcttctgc cattgctgat ggtgtaacag aaacggaggt
     3721 tttactgcct gacggcgctt cagaacatga ttattcactg cgtccatcgg atgtaaaacg
     3781 cttacagaac gcggacttag tcgtttgggt tggcccggag atggaagcgt ttatgcaaaa
     3841 accggtaagc aaattaccag gagcgaagca ggtaacgatt gcgcagcttg aagatgtgaa
     3901 accgctactg atgaaaagta ttcacggcga tgatgatgat cacgaccacg cggaaaaaag
     3961 tgacgaagat caccatcacg gcgatttcaa catgcatctt tggctttccc cagagatagc
     4021 gcgggctaca gcggttgcaa tccatggaaa attagtggaa cttatgccgc aaagtcgagc
     4081 caaacttgac gccaacctga aggattttga ggcacaatta gcctcaaccg aaacgcaggt
     4141 tggtaacgag ctcgcgccgc tcaaggggaa aggttatttc gtttttcacg atgcttacgg
     4201 ctatttcgaa aaacagtttg gactgacacc gcttggtcat tttaccgtta accctgagat
     4261 tcaacctggc gcgcagcgtt tacatgaaat aagaacacag ttggttgagc aaaaagcaac
     4321 ctgcgttttt gctgagccac agttcaggcc agcggtcgtt gagagcgtcg cacgagggac
     4381 atccgttcgt atgggaacgt tggatcccct ggggacgaat atcaaactgg gtaaaacaag
     4441 ctattcagaa ttcctgagtc aattagccaa ccagtatgcg agctgcctga aaggagatta
     4501 atgaggaagt gattacgtgc aacagatagc ccgctctgtc gccctggcgt ttaataattt
     4561 accgcgacca caccgcgtta tgttggggtc gctcaccgtt cttactctgg ccgtcgctgt
     4621 ctggcggcct tatgtttatc accgtgatgc cacgccaatt gtcaaaacca ttgagctgga
     4681 acagaacgaa attcgttcgc tcttacctga agccagtgag ccgattgatc aagctacaca
     4741 agaagatgaa gccattcccc aggacgaact ggatgacaaa atcgccggtg aagcgggcgt
     4801 gcatgaatat gttgtttcca ctggcgatac gctaagcagc attctcaatc agtatggtat
     4861 tgatatgggt gatatcaccc aactggctgc ggccgacaaa gaattgcgta acctgaaaat
     4921 cggtcaacaa ctctcctgga cattaaccgc ggacggcgaa ctgcagcgcc tcacctggga
     4981 agtgtctcgt cgtgaaaccc gaacctatga ccgtactgcc gctaacggtt ttaaaatgac
     5041 cagcgaaatg cagcaaggag agtgggttaa caatctgctg aaaggtaccg tcggggggag
     5101 ctttgttgcc agcgccagaa acgccggttt aaccagcgcc gaagtgagcg cagtgattaa
     5161 agccatgcag tggcaaatgg atttccgcaa actgaaaaaa ggcgatgaat ttgcggtgtt
     5221 aatgtctcga gaaatgcttg atggtaaacg tgagcaaagc cagctgctgg gcgtacgttt
     5281 gcgttcagaa ggtaaagatt attacgctat ccgcgctgaa gatggcaaat tctacgatcg
     5341 taacggtact ggtctggcga aaggattctt gcgattcccg acggcgaaac agttccgtat
     5401 ctcgtctaac tttaacccgc gtcgaactaa tccggtgaca ggtcgcgttg caccacacag
     5461 aggtgttgat ttcgccatgc cacaaggtac gccagtgctt tcagtgggtg acggtgaagt
     5521 ggtggttgcc aaacgcagtg gcgcagcagg ttattatgtg gctattcgtc atggtcgcag
     5581 ctacaccacg cgttatatgc acttgcgcaa gattctggtg aaaccgggac agaaggtgaa
     5641 acgtggcgac cgtatcgcgc tttccggtaa taccggacgt tcaactgggc cgcatctgca
     5701 ctatgaagta tggataaacc agcaggtcgt aaacccgctg acggcaaaac tgccgcgtac
     5761 tgaagggctg accggctccg atcgtcgcga attcctggcg caggccaaag agattgtgcc
     5821 gcagctacgg tttgattaat taacatccat tcgcagccgg tacgcagtca gtaccggctt
     5881 tttttatttg gtgcggggca agttgcgccg ctacactatc accagattga tttttgcctt
     5941 atccgaaact ggaaaagcat ggaaacgaaa aaaaataata gcgaatacat tcctgagttt
     6001 gataaatcct ttcgccaccc gcgctactgg ggagcatggc tgggtgtagc agcgatggcg
     6061 ggtatcgctt taacgccgcc aaagttccgt gatcccattc tggcacggct gggacgtttt
     6121 gccggacgac tgggaaaaag ctcacgccgt cgtgcgttaa tcaatctgtc gctctgcttt
     6181 ccagaacgta gtgaagctga acgcgaagcg attgttgatg agatgtttgc caccgcgccg
     6241 caagcgatgg caatgatggc tgagttggca atacgcggac cggagaaaat tcagccgcgc
     6301 gttgactggc aagggctgga gatcatcgaa gagatgcggc gtaataacga gaaagttatc
     6361 tttctggtgc cgcacggttg ggccgtcgat attcctgcca tgctgatggc ctcgcaaggg
     6421 cagaaaatgg cagcgatgtt ccataatcag ggcaacccgg tttttgatta tgtctggaac
     6481 acggtgcgtc gtcgctttgg cggtcgtctg catgcgagaa atgacggtat taaaccattc
     6541 atccagtcgg tacgtcaggg gtactgggga tattatttac ccgatcagga tcatggccca
     6601 gagcacagcg aatttgtgga tttctttgcc acctataaag cgacgttgcc cgcgattggt
     6661 cgtttgatga aagtgtgccg tgcgcgcgtt gtaccgctgt ttccgattta tgatggcaag
     6721 acgcatcgtc tgacgattca ggtgcgccca ccgatggatg atctgttaga ggcggatgat
     6781 catacgattg cgcggcggat gaatgaagaa gtcgagattt ttgttggtcc gcgaccagaa
     6841 caatacacct ggatactaaa attgctgaaa actcgcaaac cgggcgaaat ccagccgtat
     6901 aagcgcaaag atctttatcc catcaaataa aaaacagcct ctcaaattat tgagaggctg
     6961 tacttgataa aaaatctcaa tgagtgttct caccattttg agtttttaga ttagtacgct
     7021 attgagttaa tcgtagcgtg ataatttcaa aaggtttgaa t
//
LOCUS       DAZFDP010000211         7041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFDP010000211 DAZFDP010000000
VERSION     DAZFDP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6214
                     /gene="efa1"
                     /locus_tag="ACHJ1G_004631"
     CDS             <1..6214
                     /gene="efa1"
                     /locus_tag="ACHJ1G_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO4766351.1"
                     /translation="IFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVHTEENLLP
                     APSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEIFNKLSELPPHELLTP
                     VLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGT
                     LNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLS
                     LLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVVSSLH
                     FIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLY
                     LQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKY
                     ITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHAD
                     FLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGL
                     STRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLN
                     GDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQR
                     QATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHT
                     TSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKI
                     NLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKA
                     LSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAAS
                     VAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPER
                     GLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSI
                     SPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVP
                     DSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRG
                     GSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQSVMLKTTDGE
                     VPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLK
                     TPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLEDETQLNRFAG
                     NFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILDRLHDMGWSL
                     APEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTPGSRYIITGP
                     DTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISG
                     IDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQV
                     MTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIP
                     PYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSL
                     ILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFF
                     TEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQ
                     KEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWYTDDLSGRYK
                     SVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIP
                     QNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGR
                     VYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVS
                     KWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNA
                     PGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6386..6736
                     /locus_tag="ACHJ1G_004632"
                     /pseudo
     CDS             6386..6736
                     /locus_tag="ACHJ1G_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2090 a         1597 c         1597 g         1757 t
ORIGIN      
        1 tatatttaaa gggcaaacac tggatcgtct ttatcatgaa ggatatgtat tttctgatat
       61 caataccttg tctcgttata cactacacgg actaggaata actggtgtac atactgaaga
      121 aaacctgcta ccagctcctt catcgtcctt aattaatata ttgaaagaac attataatga
      181 agatgaaatt agtgcgaaat taccactagc atatgattac attttaaata aaaaagaatc
      241 aagctctatt cctgttgaaa tttttaacaa actttcagag ttaccaccac atgaactact
      301 cacacctgtt cttggccaga gtgttaatcc tctgggcatg ggctactcat ccgataatgg
      361 aaaaatcaca gagcaagtaa tagtcagtgg agctgatgga tttgataatc ccatatctgg
      421 acttatatat acctatcttg aagatctata taacatccat gtaaggatgc gagaaggtac
      481 actaaattca cagaatcttc gtcagcttct ggaaaactct gtttcttcat gctttttgac
      541 tgagcaaagt attaataaat tacttagtga ggcagaaaaa aggccttatc agtctttaac
      601 agaaatacat cagcatctca caggattacc aactattgcc gatgcaaccc tttcattact
      661 ttctgttgga ttacctggta cgggaaaact attgcgcagg gagcaggact atgggcgtcc
      721 accagttaca gcaattcagg attccacatt tgtactccct tataatttca aaggtattgg
      781 ttttaacgat aacattatat cctctgcacc tgtagtctcc tcattacatt ttatcgctga
      841 acatgcgaaa tatactttgt tgtcatggcc tgagttttat cgtcatcatg cacagcgatg
      901 gttcgaaatg gctaaaggat atggaagcca gaatattgat tttcaccctc agtctctatt
      961 ggtaacccaa gaaggacgct gtatgggatt agccttactt tatttacaga ctgaagatac
     1021 tgctcattat agcattctcc aggaaaacct aatgactgtg agtgcacttc atcagaccag
     1081 taatcgcgat aagttgccac tgtccaaaga tgataattcc ttaatgacaa gaacttatag
     1141 tctgattgaa atgctacagt atcagggaaa caaatatatt accaacgaat cgctactaca
     1201 taagaccgca tggaaccaag aaagaataac tttattattc aatgaaaaag gagttaagcg
     1261 agccctgata agcacgccta atcatactct ggttctgcaa caactggagg atatttaccg
     1321 gctcactgat ccaaattttg ggcatgcaga tttcctttca cctatagatg ctctgaagtt
     1381 tattgaggct atgatacaat taactccaac acttcaggaa tattatggcc tattaaacaa
     1441 agacattaat aaacatatac aagtgcatta tgccgaatca gatatggtct ggaataagct
     1501 tctgccagaa aatgatgctg gactgagcac cagaattcag cacaccacca ccgaccgtct
     1561 ggcgaatctg gctgaaccag tcgctgttgc aggtatctcc ctgccagtaa aaacacttta
     1621 tgatatcgga gccacccttg acggtcggcg catcacctct cctccaacat cggagcaaat
     1681 cccttctctg cgtctcaacg gtgatgttct gaatgattat ctgtcccgca cagttctgac
     1741 tccagaacag gctgataaca taagaaaaat actgcacact cagggaatac gcagcggtac
     1801 ccgtcccata gatccggaga tgattcgtgg gacgcaggat gacctagttt cgtcacagac
     1861 tcgtctgcaa aggcaagcaa cacgggttaa acagcaactc gccggcgtac ttgatactct
     1921 gcaacagcac ttccagaaca ttccacgttc atccggtcgt catctttctg tagagaatat
     1981 tgagctggct gatatcggaa gtgggcgttt caaccttcaa attcgagatg gagaaacatt
     2041 gcataccact tctgtggaag taccggaagt tgtgtcccgt tttcagaaac tttctaccat
     2101 gctttcagcc ctgcctgcca gtggaatcat ggatttcgac ctcggcatga gtgtggtcgg
     2161 agtcgtccag tatgcccgcc tgctacagca ggggcacgaa gacagtaccc tagccaagat
     2221 aaatctagct atggatatca agcaactttc cgaagcaact ctcggcagca tgattcagat
     2281 tgccgggaat aagtttctca atacagaagg aatccagggg ttcagactgg aaagcgccgt
     2341 cgctgaaggt atgcgctcag tagcaacccg taccggaggc acaatgggga aagccctttc
     2401 tgccagtgcc cgtgttcttg aactgcctgt actggaaaca gttctgggga catggaacct
     2461 gtacaacagc gtcattcagc tccagcaagc tacatcttat tctgagacaa tggctgcccg
     2521 ggtacagatt gcgtttgatt ccatttctct gggattaact gccgcttcgg tagctttccc
     2581 gccactaatt attgccactg gccccattgc ggctattggt atgggagctt ccagtattgc
     2641 acgtaatgtg gcacggaaag aagaacggca tacacaatgg ctggaatata aaaaattcct
     2701 gactgatggc agtaaacaca ttgttgtggc ctctccggaa agaggtctgc tggatttctc
     2761 cggaaacaaa gtttttggaa aaatggtgct ggatctgcgt cagtctcctc ctctcttgca
     2821 tggagaaagc tcttttaacg ctgaccgcaa aatcggtcat cgtccggatc tgggggactg
     2881 gcaaattcgt gagaaggtgg ggtatgccaa cagtatcagt ccctactctt ccctggcgca
     2941 cggttacgcc aacagtaaat ggccacaaac aataccgaaa attccctcgg gagaatatga
     3001 caccataatt ctgggctacg gtcaccagta tcaggccaat acggaaatag aatatctgtc
     3061 taactggatt gtatggcggg aagccgtacc agacagtact tcccgccaca aacgtcctcc
     3121 tctggaggtt cttaatagtc agtgtactgt gatagctgga gagcgtaaaa ccacagtact
     3181 tcccctgaga gtgctcagcg atctgacacc ggaatgcaca gaacaggcta tatcgttaaa
     3241 agattataaa ttcatactga gagggggaag cggtgggctg gctgttcagg tcggtggcgc
     3301 gggatattat gatattgatg caaatcctgt ggcaaaagaa aatacgctct cttttcgcgg
     3361 gctaccggaa gagtttccgc tcgcctttga tttatcaaaa caaacacagt cggtcatgct
     3421 gaaaacaacg gacggtgagg tgccggtaat gaccatcacc cagaagggaa taaacaccct
     3481 ggtcggtaca acggccggcg aaaactggct aacgggtaac gataaggaca ataccttcca
     3541 tacgagttct ggtggcggta ctgtcatctc tggtggaggg aataaccgct atattatccc
     3601 ccgcgattta aaaactccgc tgacactgac gctgtctaat agctcggtct ctcacgagat
     3661 ccttctgcca gaaacaactc tgacagaatt aaaacctgtc gcctttgagc tgagtctgat
     3721 ttactgggcc gggaacaata taaaggttca gctggaggat gagacacaac tgaaccgttt
     3781 tgccggaaac ttcagggggc ataccagtga tggcattact ctggaggcgg tttcccagga
     3841 aaatggtatt caactggggg tttcatcatg tgatgttcaa cgctggcagg ctgtttatcc
     3901 ggaagaaaac aacagaccag acgccatact ggacaggctg catgatatgg gatggagcct
     3961 ggcccccgag gtcaggttcc ggggaggaga aacatctgcc agctatgacc ccctgaaccg
     4021 tcagctcgtt taccggcttc aggagcgtta ctcggaattc aggctgaccg gcagtcgcca
     4081 ttacaccacg gctgtaaccg gaacccccgg aagccggtac attattacgg ggccggatac
     4141 agcacagata tcaccggcac aaatcatact ggccggtgat aatgaccatc cggaaacgat
     4201 tgatttgctt gaggccagtc ctgttctggt tgaagggaaa aaagacaaaa acagcgtgat
     4261 attaacgatt gctacgattc agtattccct tcaactgaca atatccggga tcgacgaatc
     4321 gctgcccggg acaacccgtg tggcaattca gcctcaggat acccgtttac tgggtgacgt
     4381 actccggatc ttaccagata atggtaactg ggtggggatt ttccggagtg gtcatacacc
     4441 aacggtaaac cggctggaaa atttgatggc actgaatcag gtaatgacgt tcctgccccg
     4501 ggtatccgga agtgcagagc aggtattatg cctcgaaaac ctaggtggcg taaggaaaaa
     4561 agtggagggg gagttactgt cagggaagct gaaaggtgcg tggaaagccg aaggtgaacc
     4621 tactgttccg gtaaatatct cagatctaag tatcccaccc tattcacgtc tgtatctgat
     4681 ttttgaaggg aaaaataatg tgttgctacg cagtaaagta catgcagctc cgttgaaaat
     4741 aacatccgca ggagagatgc agttatctga aaggcagtgg caacagcagg aacatattat
     4801 tgtcaagccc gacaacgaag ccccctcatt aatactcagt gaatttcgtc gtttcactat
     4861 ttcatcggat aaaaaatttt ctttaaaact gatgtgccat caggggatgg tccgcatcga
     4921 ccgcagatca ttatcggtca gattgttcta tctgcgtgaa cagccaggta tcggcagttt
     4981 acgtctgacg ttcagagatt ttttcacaga agtgatggat acaacggaca gggaaattct
     5041 ggagaaagag ctgagaccaa ttctgatagg agatacacac cgctttatca acgctgcata
     5101 caaaaatcat ctgaatatcc agttaggaga tggcgttctg aatctggcag acattgttgc
     5161 ggaatacgcc cgtattcaaa aggaagaaac atcaaaaata ttgtatcaat atcaaggtgc
     5221 catgaaaaaa aaaacagatg gaccatctgt ggtagaagat gccattatga ccactactgt
     5281 cacaacagat tcaggtgaac cattccctac cttccacccg tggtatacag atgatttatc
     5341 agggcgttat aagagcgtac ctatggcaag aaaagcagat actttgtatc acctgacacc
     5401 aaaaggtgat ctacagataa tatatcaggt agctacaaaa atggtgaatc aggcgatgat
     5461 tgtatccctg ccaaactacc gacacgagtg ggaaaaatat aatttaagca tcttatccga
     5521 aatccctcag aacaataata ctgttgtaca ttcaatcctc agggttaatg gccccacaat
     5581 gcaggtgcgc acaattgact acagaggaac ggatgaaaac aatcccatag tatctttttc
     5641 agatacaacc ttcatcaatg gtgaacagat gttgagttat gactcgcatt catcagggcg
     5701 agtctattcc agagaagaat atatgatgtg ggaattgcag caacgggtat cagaagcttc
     5761 cagtgcccgg acacaggatt actggctgat ggatgcagcg gtaagaaacg gagaatggaa
     5821 gatcacacca gaattattac gtcacacacc gggatatatc cggagtacgg tatcgaaatg
     5881 gtccagagga tggctgaaaa ccggcacaat actccagact ccagaagaca gaaatacgga
     5941 tgtatacctg actaccatac agaacaatgt atttagtcgt caggggggcg gctaccaagt
     6001 gtattatcgg attgatggaa tggctggtgc ggatatagcg gataatgcac caggggaaac
     6061 ccgctgcacc ctcaggcccg gaacatgttt tgaagtgaca agtgtggatg aaaggcatta
     6121 tgagtggaat atcatttatg tcacgctgaa aacctgtggc tggagccgaa atggccaaag
     6181 caaaacgccg aatggtgaca acctttttaa ctaattaaaa tctctgttaa agatgtataa
     6241 cccggcatct atgtgctcac cagcatatag atgcaatgtg attcccctga tggtcgtatc
     6301 agagatggta tgcgagcctg tacatagatt tgtgtaattg cctgattttg atatgttcaa
     6361 tccaacatca aaagcaggtt aatttatgga cgaaaaacag ttgcaggctc tggctaacga
     6421 acaggccaaa aatctcaaaa cccctgaaga tctcagccag ttcgatcgcc tgctgaagaa
     6481 aatcagcgtt gaggcagctc tcaacgctga aatgtcccac catctgggct acgataaaaa
     6541 ccagcctaaa ccgggtgcca actcccgcaa tggctattcc acaaagaccg ttatcaccgg
     6601 tgatggcccc ctggaactac gcactccgcg cgatcgtgat ggctcttttg aaccgtaact
     6661 tgtgaagaaa aaccagaccc ggatcaccgg gatggataac cagatcctgt cgttgtacgc
     6721 caaaggcctg acaaccgtaa gcgtacagcc tgaaccgtct ggtcagaatc tgacgaatta
     6781 gagtaagctg gacatcccca gatatattat ggctgccagc gtcatatcgg cataaataga
     6841 gatatccctt aacgctaacc agaaaaaaat acctataaga gcctgtacat agagttgtgt
     6901 cgttgcctga tgttgatatg tcaaatccaa catcaaatag cggtaaattt atggataaaa
     6961 aattacaggc tctggttaac gaaacacccc cctcttgctc tattcccctc tttcatcatt
     7021 tattacaata tttatttcaa t
//
LOCUS       DAZFDP010000212         7012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFDP010000212 DAZFDP010000000
VERSION     DAZFDP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            542..1531
                     /gene="araF"
                     /locus_tag="ACHJ1G_004633"
     CDS             542..1531
                     /gene="araF"
                     /locus_tag="ACHJ1G_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4766352.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1601..3115
                     /gene="araG"
                     /locus_tag="ACHJ1G_004634"
     CDS             1601..3115
                     /gene="araG"
                     /locus_tag="ACHJ1G_004634"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4766353.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3130..4116
                     /gene="araH"
                     /locus_tag="ACHJ1G_004635"
     CDS             3130..4116
                     /gene="araH"
                     /locus_tag="ACHJ1G_004635"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4766354.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4283..5083
                     /gene="otsB"
                     /locus_tag="ACHJ1G_004636"
     CDS             4283..5083
                     /gene="otsB"
                     /locus_tag="ACHJ1G_004636"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4766355.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5058..6482
                     /gene="otsA"
                     /locus_tag="ACHJ1G_004637"
     CDS             5058..6482
                     /gene="otsA"
                     /locus_tag="ACHJ1G_004637"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4766356.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6489..6917)
                     /gene="uspC"
                     /locus_tag="ACHJ1G_004638"
     CDS             complement(6489..6917)
                     /gene="uspC"
                     /locus_tag="ACHJ1G_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4766357.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1772 a         1632 c         1821 g         1787 t
ORIGIN      
        1 gttgatatta atcaatgaat ttgaattcat gatgaagtga tgattttata aaacgttttt
       61 tcatttttgc gagatggctc tcattatacg tgttctgtta attaatgagt acagtgataa
      121 ttaatggcga aatgaatgac gtgcattttc cacatctttg agttgcggtt attacaccat
      181 ttcaaaaact caacgccagg taatgcggcc tattgactgg ttaaaaagaa gacatcccgc
      241 atgggtacca aagacaacaa ggatttccag gctaatctta tggattaatc tgctgtgcat
      301 tcgacaattt gtctgacaaa ttggctttcc cttatgtctt ttcccgctaa atttatgcac
      361 gttctcactg taattctgcg atgtgatatt gctctcctat ggagaattaa tttctcgcta
      421 aaactatgtc aacacagtca cttatctttt agttaaaagg taatgctttg ttttccgatt
      481 aatttaacga atgtcattcg tttttgccct acacaaaacg acactaaagc tggagagaac
      541 catgcacaaa tttactaaag ccctggcagc cattggtctg gcagccgtta tgtcacaatc
      601 cgctatggcg gagaacctga agctcggttt tctggtgaag caaccggaag agccgtggtt
      661 ccagaccgaa tggaagtttg ccgataaagc cgggaaggat ttagggtttg aggttattaa
      721 gattgccgtg ccggatggcg aaaaaacatt gaacgcgatc gacagcctgg ctgccagtgg
      781 cgcaaaaggt ttcgttattt gtactccgga ccccaaactc ggctctgcca tcgtcgcgaa
      841 agcgcgtggc tacgatatga aagtcattgc cgtggatgac cagtttgtta acgccaaagg
      901 taagccaatg gataccgttc cgctggtgat gatggcggcg actaaaattg gcgaacgtca
      961 gggccaggaa ctgtataaag agatgcagaa acgtggctgg gatgtcaaag aaagcgcggt
     1021 gatggcgatt accgccaacg aactggatac cgcccgccgc cgtactacgg gatctatgga
     1081 tgcgctgaaa gcggccggat tcccggaaaa acaaatttat caggtaccta ccaaatctaa
     1141 cgacatcccg ggggcatttg acgctgccaa ctcaatgctg gttcaacatc cggaagttaa
     1201 acattggctg atcgtcggta tgaacgacag caccgtgctg ggcggcgtac gcgcgacgga
     1261 aggtcagggc tttaaagcgg ccgatatcat cggcattggc attaacggtg tggatgcggt
     1321 gagcgaactg tctaaagcac aggcaaccgg cttctacggt tccctgctgc caagcccgga
     1381 cgtacatggc tataaatcca gcgaaatgct ttacaactgg gtagcaaaag acgttgaacc
     1441 gccaaaattt accgaagtta ccgacgtggt actgatcacg cgtgacaact ttaaagaaga
     1501 actggagaaa aaaggtttag gcggtaagta atttgccgga aaaattcccc tctgcatgat
     1561 gcagaggggg tgtgaacgac cagtgattca cggagacgtt atgcaacagt ctaccccgta
     1621 tctctcattt cgcggcatcg gtaaaacgtt tcccggcgtt aaggcgctga cggatattag
     1681 ttttgactgc tatgccggtc aggttcatgc gttgatgggt gaaaatggcg caggaaaatc
     1741 aactctctta aaaatcctca gcggcaacta tgcgccaacc acgggttctg tagtgattaa
     1801 tgggcaggaa atgtcctttt ccgacacgac cgcagcactt aacgcgggtg tggcgattat
     1861 ttaccaggaa ctacatctcg tgccggaaat gaccgtcgcg gaaaacatct atctcggcca
     1921 gctgccgcat aaaggcggca ttgtgaatcg ctcattgctg aattatgagg cgggtttaca
     1981 acttaaacat cttggtatgg atattgaccc ggacacgccg ctgaaatatc tctccattgg
     2041 tcagtggcag atggttgaaa tcgccaaggc gctggcgcgt aacgctaaaa ttatcgcctt
     2101 tgatgagcca accagctccc tttctgcccg cgaaatcgac aatcttttcc gcgttattcg
     2161 tgaactgcga aaagaggggc gggtgatctt atacgtttct caccgtatgg aagaaatatt
     2221 tgccctcagc gatgccatca ctgtctttaa agatggacgt tatgtcaaaa cctttaccga
     2281 tatgcagcag gttgaccacg acgcgctggt gcaggcgatg gtcgggcgcg acattggcga
     2341 tatctacggc tggcaacctc gtagttatgg cgaggagcgc ctgcgtcttg atgctgtgaa
     2401 agcaccaggc gtgcgtacgc caataagtct ggcggttcgc agtggtgaaa ttgttgggct
     2461 gtttggtctg gtaggggcgg ggcgtagcga attaatgaaa ggcttgtttg gcgggacgca
     2521 aatcaccgcc ggtcaggttt atatcgacca acagccgatc gatattcgta aaccgagcca
     2581 cgccattgcc gcaggcatga tgctctgccc ggaagatcgc aaagcggaag gcattattcc
     2641 cgtgcactcc gttcgcgaca atatcaacat cagtgccaga cgtaaacatg tgctcggcgg
     2701 ttgtgtaatc aacaacggtt gggaagaaaa caatgccgat caccacattc gttcgctcaa
     2761 catcaaaacg ccgggcgctg agcaactgat catgaatctc tcaggcggaa atcagcaaaa
     2821 agccattctg ggccgctggt tatcggaaga gatgaaggtc attttgctgg atgaacctac
     2881 gcgcggcatt gatgttggtg ctaagcatga aatttacaac gtgatttatg cgctggcggc
     2941 gcagggtgtg gcggtgctgt ttgcctccag cgacttacct gaagtcctcg gcgttgccga
     3001 ccggattgtg gtgatgcggg aaggtgaaat cgccggtgaa ttgttacacg agcaggcaga
     3061 tgagcgtcag gcactgagcc ttgcgatgcc taaagtcagc caggcagttg cctgagtaag
     3121 gagagtatga tgtcttctgt ttctacatcg gggtctggcg cacctaagtc gtcattcagc
     3181 ttcgggcgta tctgggatca gtacggcatg ctggtggtgt ttgcggtgct ctttatcgcc
     3241 tgtgccattt ttgtcccaaa ttttgccacc ttcattaata tgaaagggtt gggcctggca
     3301 atttccatgt cggggatggt ggcgtgtggc atgttgttct gccttgcttc cggtgacttt
     3361 gacctttctg tcgcctccgt aattgcctgt gcgggtgtca ccacggcggt ggttatcaac
     3421 ctgactgaaa gcctgtggat tggcgtggca gcggggttgc tgcttggcat tctctgtggc
     3481 ctggtcaatg gctttgttat cgccaaactg aaaataaatg ctctgatcac aacactggca
     3541 acgatgcaga ttgttcgagg tctggcgtac atcatttcag acggtaaagc ggtcggtatc
     3601 gaagatgaaa gcttctttgc ccttggttac gctaactggt tcggtctgcc tgcgccaatc
     3661 tggctcaccg tcgcgtgtct gattatcttt ggtttgttgc tgaataaaac cacctttggt
     3721 cgtaacaccc tggcgattgg cgggaacgaa gaggctgcgc gtctggcggg tgtaccggtt
     3781 gttcgcacca aaattattat ctttgttctc tctggcctgg tatctgcgat agccggaatt
     3841 attctggctt cacgtatgac tagtgggcag ccaatgacgt cgattggtta tgagcttatt
     3901 gttatctccg cctgcgtttt aggtggcgtt tctctgaaag gtggcatcgg aaaaatctca
     3961 tatgtggtgg cgggtatctt aattttaggc accgtggaaa acgccatgaa cctgcttaat
     4021 atttctcctt tcgcgcagta cgtggttcgc ggcttaatcc tgctggcagc ggtgatcttc
     4081 gaccgttaca agcaaaaagc gaaacgcact gtctgatgct tttttccaca acaatttaac
     4141 gttttttccc accacagcca gccgccacaa cggttggctg ttcttcattg caaatggcga
     4201 cccccgtcac actgtctata cttacatgtc tgtaaagcgc gttctgcgca aaacaataag
     4261 aaaagagaag gaggagagcc gggtgacaga accgttaacc gaaacccctg aactatccgc
     4321 gaaatatgcc tggttttttg atcttgatgg aacgctggcg gaaatcaaac cgcatcccga
     4381 tcaggtcgtc gtgcctgaca atattctgca aggactacag ctactggcaa ccgcaagtga
     4441 tggtgcattg gcattgatat cagggcgctc aatggtggag cttgacgcac tggcaaaacc
     4501 ttatcgcttc ccgttagcgg gcgtgcatgg ggcggagcgc cgtgacatca atggtaaaac
     4561 acatatcgtt catctgccgg atgcgattgc gcgtgatatt agcgtgcaac tgcatacagt
     4621 catcgctcag tatcccggcg cggagctgga ggcgaaaggg atggcttttg cgctgcatta
     4681 tcgtcaggct ccgcagcatg aagacgcatt aatgacatta gcgcaacgta ttactcagat
     4741 ctggccacaa atggcgttac agcagggaaa gtgtgttgtc gagatcaaac cgagaggtac
     4801 cagtaaaggt gaggcaattg cagcttttat gcaggaagct ccctttatcg ggcgaacgcc
     4861 cgtatttctg ggcgatgatt taaccgatga atctggcttc gcagtcgtta accgactggg
     4921 cggaatgtca gtaaaaattg gcacaggtgc aactcaggca tcatggcgac tggcgggtgt
     4981 gccggatgtc tggagctggc ttgaaatgat aaccaccgca ttacaacaaa aaagagaaaa
     5041 taacaggagt gatgactatg agtcgtttag tcgtagtatc taaccggatt gcaccaccag
     5101 acgagcacgc cgccagtgcc ggtggccttg ccgttggcat actgggggca ctgaaagccg
     5161 caggcggact gtggtttggc tggagtggtg aaacagggaa tgaggatcag ccgctaaaaa
     5221 aggtgaaaaa aggtaacatt acgtgggcct cttttaacct cagcgaacag gaccttgacg
     5281 aatactacaa ccaattctcc aatgccgttc tctggcccgc ttttcattat cggctcgatc
     5341 tggtgcaatt tcagcgtcct gcctgggacg gctatctacg cgtaaatgcg ttgctggcag
     5401 ataaattact gccgctgttg caagacgatg acattatctg gatccacgat tatcacctgt
     5461 tgccatttgc gcatgaatta cgcaaacggg gagtgaataa tcgcattggt ttctttctgc
     5521 atattccttt cccgacaccg gaaatcttca acgcgctgcc gacatatgac accttgcttg
     5581 aacagctttg tgattatgat ttgctgggtt tccagacaga aaacgatcgt ctggcgttcc
     5641 tggattgtct ttctaacctg acccgcgtca cgacacgtag cgcaaaaagc catacagcct
     5701 ggggcaaagc atttcgaaca gaagtctacc cgatcggcat tgaaccgaaa gaaatagcca
     5761 aacaggctgc cgggccactg ccgccaaaac tggcgcaact taaagcggaa ctgaaaaacg
     5821 tacaaaatat cttttctgtc gaacggctgg attattccaa aggtttgcca gagcgttttc
     5881 tcgcctatga agcgttgctg gaaaaatatc cgcagcatca tggtaaaatt cgttataccc
     5941 agattgcacc aacgtcgcgt ggtgatgtgc aagcctatca ggatattcgt catcagctcg
     6001 aaaatgaagc tggacgaatt aatggtaaat acgggcaatt aggctggacg ccgctttatt
     6061 atttgaatca gcattttgac cgtaaattac tgatgaaaat attccgctac tctgacgtgg
     6121 gcttagtgac gccactgcgt gacgggatga acctggtagc aaaagagtat gttgctgctc
     6181 aggacccagc caacccgggc gttcttgttc tttcgcaatt tgcgggagcg gcaaacgagt
     6241 taacgtcggc gttaattgtt aacccctacg atcgtgacga agttgcagct gcgctggatc
     6301 gtgcattgac tatgtcgctg gcggaacgta tttcccgtca tgcagaaatg ctggacgtta
     6361 tcgtgaaaaa cgatattaac cactggcagg agtgcttcat tagcgaccta aagcagatag
     6421 ttccgcgaag cgcggaaagc cagcagcgcg ataaagttgc tacctttcca aagcttgcgt
     6481 aggagcggtt aatctcccgt aagtggaact aacagcacat cgacctcact tgaggcaata
     6541 actcttttcg ccgagcagga tgctcgcgaa aagaaactgt gattgtgatt accgcaaatc
     6601 accaaatcga aatgatgttt gtgacatact tccagaatat gttcgcttaa ctcaccgtag
     6661 gcaataaacg tcttgtcaac gggatacccc gcatcctgaa ttaacttatc aagaaagctt
     6721 tgcgtttctt cttgcatcac gctgcgtaaa tcttcaagca tcggcgcggc taattgattg
     6781 tacatttccg ggtcggaagc gagagtaatt aaactgatgt gtccctttac tggcctggcg
     6841 atagataccg cttttgccag cagttgctga ctttccggtg taaccgcaac agcgacaaga
     6901 atattgctat agctcataac ctgttcctta ttctgtgaac ttcaggtgac attaaagctt
     6961 tactcctaat ggagaaacga cgcaatccca actcggtcaa acaggtaaat at
//
LOCUS       DAZFDP010000213         6863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFDP010000213 DAZFDP010000000
VERSION     DAZFDP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..667
                     /locus_tag="ACHJ1G_004639"
     CDS             173..667
                     /locus_tag="ACHJ1G_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766358.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            complement(698..1060)
                     /locus_tag="ACHJ1G_004640"
     CDS             complement(698..1060)
                     /locus_tag="ACHJ1G_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO4766359.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(1063..1335)
                     /locus_tag="ACHJ1G_004641"
     CDS             complement(1063..1335)
                     /locus_tag="ACHJ1G_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766360.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1769..2065)
                     /locus_tag="ACHJ1G_004642"
     CDS             complement(1769..2065)
                     /locus_tag="ACHJ1G_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766361.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(2106..2813)
                     /locus_tag="ACHJ1G_004643"
     CDS             complement(2106..2813)
                     /locus_tag="ACHJ1G_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4766362.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            2925..3149
                     /locus_tag="ACHJ1G_004644"
     CDS             2925..3149
                     /locus_tag="ACHJ1G_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4766363.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3259..3537
                     /locus_tag="ACHJ1G_004645"
     CDS             3259..3537
                     /locus_tag="ACHJ1G_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO4766364.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            3573..3734
                     /locus_tag="ACHJ1G_004646"
     CDS             3573..3734
                     /locus_tag="ACHJ1G_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766365.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            3721..4542
                     /locus_tag="ACHJ1G_004647"
     CDS             3721..4542
                     /locus_tag="ACHJ1G_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4766366.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            4539..5915
                     /locus_tag="ACHJ1G_004648"
     CDS             4539..5915
                     /locus_tag="ACHJ1G_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4766367.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            6002..6217
                     /locus_tag="ACHJ1G_004649"
     CDS             6002..6217
                     /locus_tag="ACHJ1G_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766368.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            6683..>6863
                     /locus_tag="ACHJ1G_004650"
     CDS             6683..>6863
                     /locus_tag="ACHJ1G_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766369.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NEWWTLWQASRAAIEL"
BASE COUNT         1861 a         1484 c         1648 g         1870 t
ORIGIN      
        1 ctaatttcat tttctggcga ccaacacaag tcacacccat ttcactgcgt ggcttgcggt
       61 agtaaagatt gtgcctgtct tttaaccaca tcaggctcgg tggttctcgt gtacccctac
      121 agcgagaaaa atagtaaaat cctcttaccc ctacagtaag agagtaattt atatggatgt
      181 gttatctggt ataactgccg cgaaacaggc ttatgatctg ctgaagacaa taaaagaaac
      241 cagagacgat gctgttatcg ctaaagctat tggtgatcta caccaaagaa taactgactt
      301 acagatgctt aatgcggagc tctctggcct ttatcaggcc gagaaggaga tcgcaatgaa
      361 gcttcgagat gaaaatagaa aaatcaagat gtttgttgtg caagctgaga attatgaact
      421 tcatacaact gaaggtggtt ctgtcgtgta tcgacctaag agtcattccg atccttctat
      481 tcagcagcat aacctttgtg cacattgctt cggcgaacat aaaatatcga tacttcaacc
      541 aagcacggtt actataaaat ctaatgggtt ctttgtgcat tcatgtcctc gctgtaagaa
      601 tgaataccgg atgtatagag cgccagaccc taagcctgta tatgtaccgc cgctgacaaa
      661 ttattaattt tattcctgaa atggcctctt caccccctta ctttgtcagg gggatatctc
      721 cttcagtttt gaccattcgc cttaatactt tccttaagtc gatgtaaagt tgaaggtctc
      781 catttgctgc ggcatcagcc attttttgcc tgacaagcag taatgtttca tacggctcaa
      841 taagaatatc gtcatgagta attaggtgaa gcgttgccgc atcaactatt cctagagctg
      901 cgccaagtat caaaaattcc ctgctatttt tgtcgcatga ggagataagc gtatttagcg
      961 catacctaat attctttata gctgttgtta atgctgcaat ttcttctatg gcgtcttctc
     1021 caatgagctt tttaagctca tatttttctt cctgacccat aattacctcg ccgtcagttg
     1081 ttttgatttc cggtagcctg ccgcgtaaat ggctacgttt ggcaggcaaa tacttccact
     1141 gcattcatct gccttcttgc agcgaaggct tccgagtgat gctgctttat ctgctctgac
     1201 gcaaccagag agctttagcg caatttttcg tgccaatcgc tgttcttgca ttgcctgttc
     1261 acgttgagcc tgtctgcgtg ctctgcggcg atttctggcg ttatcgtcag ccagatatgt
     1321 aatgactact gtcatgttga cctccgatga ttgactttgg cggtgacgcg ccgcgtgctt
     1381 atcttccggt tgccgtcttg cagctgcact tcacgtcacc ccaaagccaa ctactctttg
     1441 gttcccgcat ttcggcggga caatcccatc aatgttaaag agcctgccaa tctgttccgt
     1501 ttggcttcca gcgtcctgct gatggcttaa agataactta ggttataggt gtggtcaata
     1561 acctaattta tattttatgg taaataggtt ataagtgatg gataacaaag gtattttatt
     1621 tttgtaaatg ttgctgattg attggtgttt gagggcttgc gtgcggggtg aaggtgttac
     1681 ctttggcttg atgcttgtct atgatgagga tggttgattg ggtggtgaac ggcaggaaaa
     1741 gaaaacccgg cgctgaggcc gggtgttttt agtcttttct tttgcttagc atttcgtcga
     1801 tttctaagtc aatacgatat tgatctattg cttttctctc gtttggggtt ggtattttat
     1861 actgttcaat tagatctgtt gtgtatttta tttcatctaa tgtgattttt atatcagaga
     1921 gaatctcttt tatatcaatt atatgctctt cttcttttgc acgattagag ctatgttcaa
     1981 tcatttgaga tagcttttta ctaatgctta gcaaaacaaa aagaatgatg accaaaacaa
     2041 caacaaaaac tatcagaaat tccattatcc ctccgcactt ccgtaagtct tcttctgatt
     2101 atcggttacc aactatgaga cgaccagaat actctgccaa taatccttac ggtttcatga
     2161 aattcatctc tatccattac ttcatccggg tactcttcgc gatttattga tctgattatc
     2221 accgatgtag gggtggcgat taatgttttt actcgtaaca aatcagactg gcaaatagcg
     2281 taggttttac catctctgat tgtggtatct tgcgtgttaa caccaacaac atcgccatcg
     2341 tgaagcgttg gttccatgct ttgccctaca accctaacta gcttggctga tctttcagat
     2401 actcccatct ttttcagata gtgctttctg aaaaccaaag agaactccga tgattcctct
     2461 agctcgcagc taccgcttcc agctgaaagc gaaacgttaa gaagaggcaa cgcaacaaac
     2521 tcgtcatcgt ttcttttaat gtcttcccat accacagctt ttaaagatga atcacggaca
     2581 ttggatggtt cttcatgtgc accatccctc atttcaccaa taccagaact aagccattca
     2641 gggcgcactt ttaaagcatt ggctaattca accatcttgc gagatccgtt tgttttaccg
     2701 gacgacatct tctgtatggc tggctgagat attccaacca tgtcagcaag ctgtgattgt
     2761 gatacccctg ctgagctcat ggctgcattt agtctttctg cgaatgtttt catacccaca
     2821 aatctataac tacggttatc caaagtaaaa taacaaaggt tattgctatt ttttataact
     2881 tgagttatct ttggttataa gtaatgacca caagaggtat gctcatgaat ttagtaattc
     2941 aacgagcctt gaaaattgtc ggtagccaaa agcgccttgc cgacaagtgt ggtgtaacgc
     3001 agccagcagt acacaaatgg ctgaaaggcg ggttggtctc tccagagaaa gttaccgcca
     3061 tcgttaacgc cactggaggg cagatcaagg cttacgaaat tcgccccgat ttgccacacc
     3121 tgtttccaaa accgaatcag gcagcataag taacaccgct ctttaacagt catggtcctc
     3181 attcccgccg aaatgcggga atacaacgcg cataagttga tgcgcataac ttcttatttg
     3241 ttaaggaaat acttacatat ggaacttaca agtactcgca agaaagcgaa tgcaattaca
     3301 agcaacatcc tgaatcgaat tgctgtacgt ggtcagcgaa aggttgctga tgcattaggg
     3361 atcaatgaat cgcaaatttc gcgatggaaa gacagcttta tcccaaagat ggccatgctt
     3421 ctggctgtgc tggagtgggg tgttgaagac gaggaattag cggagctggc aaagaaagta
     3481 gccatggtgc tgacaaaaga aaagcctcaa gactgcggca acagttttga ggcctgattg
     3541 tagaaagact gtatcaatcc acaggagtaa ttatgacaaa acgtcgtaag aaataccagg
     3601 aaaaagaaga gattcgacac cctgattcac ctgagggatt agtggtagcc gcagcaaata
     3661 acagggcgtt cgcagagcgc cttgttggtg tttacagact agccaaagca ggagtgaaac
     3721 atgggcgtcg ttaagttagc tgattacagg cctcaactgg aggtcgtgga gcatcgcgtg
     3781 gcagataccg aagatggttt catgcgcgtt gctaacgaga ttatcgacag tctgctgatg
     3841 gctgatttaa ccgtccggca gatgaaggtg atgctcgcta tcatgcgcaa gacatacgga
     3901 ttcaataagc cgatggatcg actcacaaac acgcagatag cagccatgac aggtattcat
     3961 cacactcatg tttgcgctgc caagcgccag cttattgagc gtaaattcct cattgctgat
     4021 ggcgtgaaaa tcggagtgaa caaggtggtt tctcagtgga ttagccagga cagcttaaca
     4081 ttagctaaaa cagctaataa aacattagcc gagtcggcta atgggtataa gccaagtcag
     4141 ctaaacacaa aagacaatat acaaaagaca ataaatacaa ataccccctt accccctaac
     4201 gggggcggcg atgggcaggt taaacctgaa cgtcgcaagg cagaacgaat cgactacgaa
     4261 tccttcctga acgcctacaa caccgaagtc ggtgacagac ttccacacgc tgttgcggtc
     4321 aacgagaaac gaaaacgccg cctgaagaaa atcatcccgc aactgaaaac gccaaacgtg
     4381 gacggtttca gagcgtatgt cagggcgttt gtgcatcagg ccaagccgtt ttacttcgga
     4441 gacaacgaca cgggctggac ggcagatttt gattacctgc tgagggaaga ctcgttaacg
     4501 ggagttcggg aagggaagtt tgcagacagg gggattgcat gagacaggat atcgaagcga
     4561 gcgttatcgg tggcttgctg attggtggat taacaccaac cgccagcgac gttctggcaa
     4621 cgctggagcc ggaagcgttt tcaattccgc tctaccggaa agccttcgaa gttatccgta
     4681 agcaggcgag aaacagaaac ctaatcgacg cgctgatggt tgccgaggcg tgcggagagg
     4741 agcatttcac gtcaatcctg atgaccagca aaaactgccc gagtgccgca aacctgaagg
     4801 gatatgccgg aatggtcgcg gataactatc accgccgtct ggtgctggaa atcatggacg
     4861 aaatgcgtga accaattcag agcggaacca tcgatacatc gagtcaggcg atggacgagc
     4921 ttgtaaagcg tctctcagcc atcagaaagc cccgtgacga ggttaaacct gtacggttag
     4981 gggaaatcat cactgactac actgacacgc ttgacaggcg tctgaggaac ggagaagagt
     5041 cagataccct gaagaccgga atcgaagaac ttgacgccat caccggaggg atgaacgcgg
     5101 aagacctggt gattatcgcc gctcgtcctg gtatggggaa aaccgaactg gcgctgaaga
     5161 ttgccgaagg tgttgcaagc cgcgttattc ctggttctga cgtccggcgc ggagtgttga
     5221 ttttctcgat ggaaatgagc gcattgcaga ttgcagagcg gagcattgcc aacgccggga
     5281 ggatgtcggt tagcgtactg cgaaatcctg catcgatgga tgacgaaggc tgggcgcgtg
     5341 ttgctaacgg catgagtcag cttgcagatt tggatgtatg ggtagtcgat gcctcgcggt
     5401 tatcggtaga agaaattcgc tcaatcgcag agcggaacaa acaggaaaat ccaaacctgt
     5461 cactcatcat ggcggattat cttggcctga ttgagaagcc gaaagcagac cgcaacgacc
     5521 tcgcaattgc tcacatctca ggaagcctga aggcgatggc gaaagacctg aaaacgcctg
     5581 ttatctccct aagtcagctt tcgcgcgatg ttgagaagcg accaaacaaa cgcccgacaa
     5641 acgcagattt gcgtgattca ggaagcattg agcaggacgc agactcaatc atcatgctct
     5701 atcgggaagc ggtatatgac gagaacagta gcgccgcacc atttgctgaa atcattgtga
     5761 cgaaaaaccg gtttggccca cttggtacgg tttaccagcg gttctgtaac ggacactttg
     5821 ttgcatgtga ccaggatgaa gccagacaga tttgcacagc atcaaatgca cctgcttcgc
     5881 gtggcagacg atatgcacaa ggggctgacg tatgaccatc tacatcactg agctaataac
     5941 aggcctgctg gtaatcgcag gcctttttat ttgggggaga gggaattgga ggctttaaga
     6001 aatgagtacg atagctgagc ttgtcagggc taattttcgt gaagagttgg tgcgttggta
     6061 tcggtatcgt tcatcgtcca gtttgccgat tgatgagttg tatgagcatt cacctgctgc
     6121 acgacgctat ccgcgtgacc gtgtccttcg acggttgttc aaactcaaca atgagtttca
     6181 gcgcaacaga ataatccgga gtctggattt aaagtgaagg agtgagcatg agtcgaagaa
     6241 gtagcttttt ggggtttgta atattcctgt cctgcactgg ttacatcgta atctggtcaa
     6301 tttcgaacat tgaccgtggc ggggaatatc tcattgtaat gttctttcct ttgtttcttg
     6361 ggtggtacgc cgcaaggttg ctggaagaat ggggttacag gcataaaaaa taaaggagtg
     6421 ttcagtgaag caaacccttt tttattggat taagagcgag gggtaagtac cgatggtaaa
     6481 tgcatttatt tgtagtttat ttcttgtcgc gatttttcat ggattccttc tgatgatgag
     6541 ttttgttctc tggaataatg gatatcgcat attgggagta ggttttgttt tacggttttc
     6601 agttgtctgc gcgttgctac cgataattat ggcgactatc aaatattatt ggtaacccaa
     6661 aaaatcatcg atggagagtg atatggacga atcaagaaag cagtttgagg aatacgttgc
     6721 caaaaaattg agattaccat tcgagatgat aaccgaggca agaaatggtg ataggtactt
     6781 cgcattttca agcatggata ttcgtcactc cttaaatgag tggtggactt tatggcaggc
     6841 atcgcgagca gctattgaac tgg
//
LOCUS       DAZFDP010000214         6828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFDP010000214 DAZFDP010000000
VERSION     DAZFDP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..1327
                     /gene="acrE"
                     /locus_tag="ACHJ1G_004651"
     CDS             170..1327
                     /gene="acrE"
                     /locus_tag="ACHJ1G_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4766370.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1339..4443
                     /gene="acrF"
                     /locus_tag="ACHJ1G_004652"
     CDS             1339..4443
                     /gene="acrF"
                     /locus_tag="ACHJ1G_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO4766371.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4696..4917
                     /locus_tag="ACHJ1G_004653"
     CDS             4696..4917
                     /locus_tag="ACHJ1G_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766372.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5348..6373
                     /locus_tag="ACHJ1G_004654"
     CDS             5348..6373
                     /locus_tag="ACHJ1G_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4766373.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6441..>6828
                     /locus_tag="ACHJ1G_004655"
     CDS             6441..>6828
                     /locus_tag="ACHJ1G_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4766374.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIE"
BASE COUNT         1655 a         1628 c         1751 g         1794 t
ORIGIN      
        1 atattaatct ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta
       61 catcgacgaa tgataatttg taggatagca aactgtattt ttctttctgc gagttaacgc
      121 gttacctttc tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      181 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      241 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      301 gaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      361 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgtaatttca ctgaaggcag
      421 cgatgtacaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      481 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      541 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      601 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      661 aagcgctcgc atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      721 caaatcgacc gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      781 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      841 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      901 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt
      961 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1021 tacgcttttg ccgggtatgt ttgtgcgtgc acggattgat gaaggcgtcc aacctgacgc
     1081 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1141 tgttaacgat aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1201 taaatggttg attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1261 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1321 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1381 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1441 tatccaacca ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg
     1501 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac
     1561 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1621 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1681 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1741 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1801 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1861 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1921 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     1981 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt
     2041 attgctcaaa cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2101 agtgacggct cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2161 tataacgtta tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca
     2221 accggcgcga atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2281 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag
     2341 ctttctattc acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg
     2401 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2461 gtgttgttag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2521 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2581 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca
     2641 atgtcgcaaa tccagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt
     2701 ccgatggcat tcttcggcgg ttctactggg gcaatttatc gccagttctc tatcaccatc
     2761 gtttcggcaa tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca
     2821 acgctgctta aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg
     2881 tttaatacta ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc
     2941 ggttccacag gacgatattt actgatctat gcgctgattg ttgcaggaat ggtggtgttg
     3001 tttttacgtc ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgaccatg
     3061 attcagttac ccgctggcgc gacgcaagag cggacgcaaa aagtgttgga tcaagtgacg
     3121 gattactatc tgaagaacga gaaagcgaac gttgaaagtg tctttacggt taacggcttt
     3181 agcttcagcg gccaggcaca aaacgccggt atggccttcg tcagtctgaa accgtgggaa
     3241 gagcgtaatg gtgatgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattt
     3301 ggcaagatcc gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc
     3361 acggcaacgg gtttcgactt tgagttaatt gatcaggctg ggctgggtca cgatgcccta
     3421 acccaggccc gtaaccagtt gcttggtatg gcggcgcaac atcctgccag cttagtcagc
     3481 gtgcgtccta atggcctgga agacaccgcg cagtttaaac tggaagttga ccaggaaaag
     3541 gcgcaggcat taggtgtttc actttctgac atcaatcaga ccatttcaac ggcgttgggt
     3601 gggacttacg ttaacgactt catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg
     3661 gatgccaaat tccgtatgct gccagaagat gtcgataaac tttatgtccg cagcgccaac
     3721 ggcgaaatgg tgccattctc ggcctttacc acttcacatt gggtgtatgg ctctccgcga
     3781 ctggaacgct acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc
     3841 agttccggcg atgccatggc gttgatggaa aaccttgcgt caaaattacc tgctggcatt
     3901 ggttatgact ggacgggtat gtcgtatcag gaacgcttat cgggaaacca ggctcccgct
     3961 ctggtagcaa tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg
     4021 tcaattcctg tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctgctggcg
     4081 gcgacactct ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt
     4141 ggcttgtcgg ccaaaaacgc tattttgatt gttgagttcg ctaaagatct catggagaaa
     4201 gagggtaaag gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc
     4261 ctgatgacct ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgcc
     4321 ggcagtggcg cgcagaacgc agtgggtatc ggggtaatgg gcggaatggt ctctgcaacg
     4381 ttgctggcaa tcttcttcgt accggtgttc tttgtggtga tccgccgttg ctttaaagga
     4441 taaatcagaa acataaaggc actttcgggt gcctttatta ttttcagtga aacccataaa
     4501 aattaataag atattcttcc actcactctt taaaaacttt ctatagttcc cgctcccttc
     4561 actattttta caattcacat aatttgtcat tattcattcc gcaatattta taacctccgg
     4621 tgataaaatg gcattcagct cgttaataag agagttaact tattaagcgt tagcgtttat
     4681 tactgaggta acaccatgaa aagattaatt cctgtggcat tgctcaccgc attgctggcg
     4741 ggctgtgctc acgattcgcc ctgcgttccg gtttatgacg atcagggtcg tctggttcac
     4801 accaatacct gtatgaaagg cacgactcag gataactggg aaacggctgg ggctattgcc
     4861 ggcggggcag cggctgttgc tggcctgacg atggggatca tcgctctgtc gaaataactg
     4921 aacagggcgc ggagtttcgc gctctgttta acgatacagt atctttacat aagataaaat
     4981 attgctatat tctgctggct aattctctgc tgatttagca tgttatatcg cgttatttcg
     5041 ctgctttcga aataacgcat caatttaact ttcacacttc ccaccagaaa tatactcacg
     5101 ctaacaatca ttattctgct tagtaaaaca cgcgtaaata ttttgcgcca aaatgtggcg
     5161 catgtttcat tttcgcacca ttgcggggcg ctgtttttat tatttcctgt ctacactcag
     5221 cttattgcga cgcgaaatcg ggcgcaaaag ctggcactac ttttgcttat aagaggatgg
     5281 ccacagacag gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg
     5341 aagcattatg aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt
     5401 tgcaaatcag gcgcatgctg gagcgacgct tgatgccgtt cagaaaaaag gttttgtgca
     5461 atgcgggatc agtgatggat tacctgggtt ctcttatgcc gatgcagacg gtaagttttc
     5521 aggtattgat gttgatgttt gtcgtggtgt tgccgctgct gtatttggtg acgacacgaa
     5581 agtgaaatat accccactca cagcaaaaga acgcttcacg gctttacagt caggggaagt
     5641 ggatttgctc tcccgtaata cgacctggac ttcatctcgc gacgccggga tgggaatggc
     5701 atttactggc gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct
     5761 aaaaagcgcg aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga
     5821 actcaacgtt gccgactatt tcaaggcaaa caatatgaag tacacaccgg tgactttcga
     5881 tcgctctgac gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga
     5941 tcagtcgcaa ctgtatgccc tgcgcatcaa attaagcaac ccagctgaat ggattgtctt
     6001 accggaggtt atctctaaag aaccgcttgg gccggtagtt cgtcgtggcg atgatgaatg
     6061 gttctcgatt gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa
     6121 ttcccagaac gtcgatgaaa aagcggctaa tccagcaacg cctgatatgg cacatctgct
     6181 gggtaaagaa ggcgattacg gcaaggatct gaagttggat aataaatggg cctataacat
     6241 catcaaacag gtgggtaact actcggaaat tttcgagcgt aatgtaggtt cagaaagccc
     6301 gctgaaaatt aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc
     6361 gcccgtgcgt taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg
     6421 ttatgctttc cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctcct
     6481 ttgccaaccc tacggttcgt gcctggttat tccagatcct tgccgttgtt gctgttgtcg
     6541 gcattgttgg ttggttattt cacaacactg taacgaatct cagtaatcgt ggcattactt
     6601 caggttttgc ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt
     6661 accagcaggg cgacacctac ggacgcgttt ttattgtcgg cttactcaat acgctactgg
     6721 tttctgcatt gtgtattgtg ttcgcttctg ttttgggctt ctttatcggt ctggcgagac
     6781 tttcggataa ctggctgcta cgaaagcttt ccacaattta tattgaga
//
LOCUS       DAZFDP010000215         6792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFDP010000215 DAZFDP010000000
VERSION     DAZFDP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(194..1123)
                     /gene="fdhE"
                     /locus_tag="ACHJ1G_004656"
     CDS             complement(194..1123)
                     /gene="fdhE"
                     /locus_tag="ACHJ1G_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4766375.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1120..1755)
                     /gene="fdoI"
                     /locus_tag="ACHJ1G_004657"
     CDS             complement(1120..1755)
                     /gene="fdoI"
                     /locus_tag="ACHJ1G_004657"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4766376.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1752..2654)
                     /gene="fdoH"
                     /locus_tag="ACHJ1G_004658"
     CDS             complement(1752..2654)
                     /gene="fdoH"
                     /locus_tag="ACHJ1G_004658"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4766377.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2667..5717)
                     /gene="fdnG"
                     /locus_tag="ACHJ1G_004659"
     CDS             complement(2667..5717)
                     /gene="fdnG"
                     /locus_tag="ACHJ1G_004659"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5130..5132),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4766378.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5911..6744
                     /gene="fdhD"
                     /locus_tag="ACHJ1G_004660"
     CDS             5911..6744
                     /gene="fdhD"
                     /locus_tag="ACHJ1G_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4766379.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1487 a         1840 c         1838 g         1627 t
ORIGIN      
        1 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
       61 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      121 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      181 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
      241 catagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg
      301 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
      361 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
      421 tctgctcgca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
      481 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
      541 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
      601 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
      661 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
      721 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
      781 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
      841 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
      901 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
      961 gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1021 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1081 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1141 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1201 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     1261 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     1321 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     1381 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     1441 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     1501 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     1561 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     1621 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     1681 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcaggatggt
     1741 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     1801 tccgcacggt tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca
     1861 aagccaacag ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     1921 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     1981 attacgtgtg taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg
     2041 gttttcagct cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2101 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2161 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     2221 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     2281 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga
     2341 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     2401 acttccgaga agcgcattac cgtccacgat ttggcgctta aatcattggg gttgtcgtac
     2461 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     2521 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     2581 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     2641 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     2701 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag
     2761 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     2821 ttgccgttcg ctttcagtgt acgaatacgt ttggtcacca ccgctttggc cttgatatag
     2881 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     2941 gactccccga tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg
     3001 gtccagtagt ggaaatgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3061 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3121 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3181 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     3241 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     3301 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     3361 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     3421 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     3481 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     3541 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     3601 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     3661 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     3721 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     3781 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     3841 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     3901 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     3961 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4021 ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgcct
     4081 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4141 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4201 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     4261 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     4321 cctggcagac tctgcgacag tagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     4381 cgcagggcgt taacgccgcc gccggccatc cccatattgc cgagcagcag ctggatcatc
     4441 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     4501 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     4561 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     4621 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     4681 ccgttttcgt ccagttcata agtccaggag gatttatcgt acttgcgttt ttccgcgtcg
     4741 tagccggtga acaggccatc ttcaaagccg taatcctcac gcactatcag gctggcgttg
     4801 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     4861 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc agctaccgcc
     4921 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     4981 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5041 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5101 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5161 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     5221 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     5281 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     5341 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     5401 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     5461 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     5521 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     5581 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagcgctac gctgggtgca
     5641 aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     5701 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     5761 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     5821 cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     5881 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcaaaa agaaatcgaa
     5941 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6001 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6061 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6121 ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6181 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     6241 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     6301 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     6361 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     6421 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     6481 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     6541 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     6601 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     6661 gagcgttgta atctgacgct ggtgggtttt tgtaagccgg gtcgggcaac ggtttatacc
     6721 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     6781 acttattcat tt
//
LOCUS       DAZFDP010000216         6771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFDP010000216 DAZFDP010000000
VERSION     DAZFDP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..86
                     /locus_tag="ACHJ1G_004661"
     CDS             <1..86
                     /locus_tag="ACHJ1G_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4766380.1"
                     /translation="VNTGGNKRQVPAKRQNRGSRNSTDDGG"
     gene            112..639
                     /locus_tag="ACHJ1G_004662"
     CDS             112..639
                     /locus_tag="ACHJ1G_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4766381.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQ
                     QPSEPAYDFDDDIPF"
     gene            695..928
                     /locus_tag="ACHJ1G_004663"
     CDS             695..928
                     /locus_tag="ACHJ1G_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO4766382.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            992..2950
                     /locus_tag="ACHJ1G_004664"
     CDS             992..2950
                     /locus_tag="ACHJ1G_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4766383.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3005..3439
                     /gene="psiB"
                     /locus_tag="ACHJ1G_004665"
     CDS             3005..3439
                     /gene="psiB"
                     /locus_tag="ACHJ1G_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4766384.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            3436..4155
                     /locus_tag="ACHJ1G_004666"
     CDS             3436..4155
                     /locus_tag="ACHJ1G_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4766385.1"
                     /translation="MSVHSRALVPLCTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPDDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLTGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREVA"
     gene            4155..4673
                     /locus_tag="ACHJ1G_004667"
     CDS             4155..4673
                     /locus_tag="ACHJ1G_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766386.1"
                     /translation="MQNARHPKTHRELCELACRFLRNNGFKVVFSDRFHAWTSCGEHP
                     DALGFRNGASCLIEVKCSRSDFLADRKKVFRSEPEKGMGDWRFYLCEPGVITVADLPA
                     GWGLLYAINGRVRKVHGWPGNHQWVVTVAKPFGANKQAECDYLFSALRRLEIRGVTEN
                     GADADRVQTYGL"
     gene            4791..4943
                     /locus_tag="ACHJ1G_004668"
                     /pseudo
     CDS             4791..4943
                     /locus_tag="ACHJ1G_004668"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4888..5010
                     /locus_tag="ACHJ1G_004669"
     CDS             4888..5010
                     /locus_tag="ACHJ1G_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4766387.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(5304..5559)
                     /locus_tag="ACHJ1G_004670"
                     /pseudo
     CDS             complement(5304..5559)
                     /locus_tag="ACHJ1G_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5855..6178
                     /locus_tag="ACHJ1G_004671"
     CDS             5855..6178
                     /locus_tag="ACHJ1G_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766388.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            6237..6479
                     /locus_tag="ACHJ1G_004672"
     CDS             6237..6479
                     /locus_tag="ACHJ1G_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766389.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEICDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
     gene            complement(6679..>6771)
                     /locus_tag="ACHJ1G_004673"
     CDS             complement(6679..>6771)
                     /locus_tag="ACHJ1G_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766390.1"
                     /translation="NNNIISNRQMTGMKRQKHTAEHPRNERGGA"
BASE COUNT         1519 a         1744 c         2091 g         1417 t
ORIGIN      
        1 tggtgaatac cggagggaac aaaaggcagg tccccgcaaa acgtcaaaac cgcggctccc
       61 gaaactcaac agatgatggg ggctgaaaac atcaacagaa ggagacacat catggcagtt
      121 cgtggcatta acaaggtcat tcttgtcggt cgtctgggaa aagatccgga agtccgttac
      181 atcccaaacg ggggcgcagt ggcaaacctg caggtggcca cgtcagaaag ctggcgtgac
      241 aaacagacgg gggagatgcg ggagcagaca gaatggcatc gcgtggtgct gttcggcaag
      301 ctcgcggaag tggctggtga atatctgcgc aagggcgcgc aggtctacat cgaggggcaa
      361 ctccgtaccc gtagctggga agataacggt atcacccgtt acgtcactga aattcttgtt
      421 aagaccacgg gcaccatgca gatgctggga cgtgccgcag gtactcagac tcagccggaa
      481 gaggcgcaac agttcagcgg tcagcctcag ccggaatcac agccggaacc gaaaaaaggt
      541 ggcgcgaaaa cgaaaggccg tggacgtaag gccgcgcagc cggagcctca gcagccgtct
      601 gaacccgcgt atgacttcga tgacgacatt ccgttctaac gaaagacagt gacaaccgcc
      661 ccgtccagtg cggggcatca ccggagatat gaggatgagc gaatatttca gaatatttca
      721 ggggctgcct gacggctcct ttacccgcga acaggctgaa gccgttgccg cacagtaccg
      781 gaatgtcttt atcgaggatg atcagggaac gcattttcgc ctggttgtcc gcaataacag
      841 tggcctggtc tggcgtacct ggaattttga ggacggtgcc gggtactgga tgaaccatgt
      901 catccggaat ttcggcattc ttaagtaaga gaggtgccgg acgggaacgc gtccggcaga
      961 catacaacca tcccggtcaa aggagaaata tatgtcagtt acagagtcta agacaaaaac
     1021 ggagcgtaaa tccagccgta aacctgcaaa aacgcaggaa acagtcctgt ctgccctgct
     1081 ggagcagacg gaggaagtga gcgtgtcgct ggcctcgctg attaaatcac cgctgaatgt
     1141 gcgcacggtg ccgtattccg cagagtccgt cagcgaactg gcggagtcca ttaagggcgt
     1201 tggcctgttg cagaatctgg ttgttcatgc cctgcctggt gaccgccacg gtgtcgccgc
     1261 aggtggtcgc cgactggcag cactcaacat gctggcagag cgtggcatcc ttccggctga
     1321 ctggcctgta cgcgtgaagg ttattccgca ggagctggcg acggctgcat caatgactga
     1381 aaacggtcag cgtcgggata tgcaccctgc cgaacagatt gccggatttc gtgcaatggc
     1441 gcaggaaggc aaaacgcctg cacaaatcgg tgatttgctg ggctattcgc cccgccacgt
     1501 tcagcgaatg ctgaaactgg cagaccttgc gcctgtcatc ctcgatgcgc tggcagaaga
     1561 ccgcatcacc acagagcact gtcaggcgct ggcgctggag aacgacaccg cgcgtcaggt
     1621 gcaggtgttt gaagccgcct gccagtcggg atggggcggt aaaccggaag tgcagaccat
     1681 tcgtcgtctg gtgaccgaaa gtgaagtggc ggtggcgggg aacagtaaat tccgcttcgt
     1741 gggggctgat gccttctcgc cagacgaact gcgcaccgat ttgttcagcg acgacgaagg
     1801 tggctatgtg gactgcgtgg cgctcgatgc cgccctgctg gaaaaactcc aggctgtcgc
     1861 agagttcctt cgggaagccg aaggctggga atggtgcgcc ggacgcatgg agcctgtcgg
     1921 tgagtgccgt gaggatgccg gaacataccg ctgtctgccg gagccggaag cggtgctgac
     1981 ggaggcggaa gaagaacgtc tgaacgaact gatgacgcgt tacgacgcgc tggaaaacca
     2041 gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg attgactgca tggcgaaggt
     2101 cagggcgtgg acgccggaga tgcgtgccgg aagtggcgtg gtggtgtcct ggcgttatgg
     2161 aaacgtatgt gtccagcgtg gtgtgcagtt gcgcagtgag gatgacgtgg ctgacgacgc
     2221 tgaccgtacg gaacaggtgc aggagaaagc gtcagtggag gaaatcagtc tgccactgct
     2281 gacgaaaatg tcttccgaac gcacgctggc agtccaggca gcactgatgc agcaaccgga
     2341 caaatccctg acactgctgg catggacgct ctgcctgaat gtgtttggca gcggagcgta
     2401 cagtaaacca gcacaaatca gcctggaatg taaacattat tcgctgacca gcgatgcgcc
     2461 atcggggaag gaaggtgccg cattcatggc gctgatggca gaaaaagccc gtcttgctgc
     2521 cctgttaccg gagggatggt cacgggacat gacgacattc ctgtcactca gtcaggaggt
     2581 gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt aacggtgtcc agacccgtga
     2641 gtgtggtcac acgtcacgca gtccgcttga cacgctggaa accgccatcg gctttcacat
     2701 gcgagactgg tggcagccga caaaagcaaa cttcttcgga cacctgaaaa agccgcagat
     2761 tatcgcagcc ctgaatgatg ccggactgtc cggtgccgca cgggatgcag agaagatgaa
     2821 gaaaggtgat gcggctgaac atgcagagca ccatatgaaa gacaaccgct gggtgcctgg
     2881 ctggatgtgt gcaccacatc cacagacaga taccactgaa cgcactgata acctggctga
     2941 tgccgcctga tgaacaacca caccgccccg ccggagacgg ggcggcagca agggagatac
     3001 cgtgatgaaa actgaactga ccctgaatgt attacagacc atgagcgcac aggaatatga
     3061 agatatccgg gctgccggaa gtgatgaacg tcgtgagctg acacacgccg tgatgcggga
     3121 actggatgcg ccggacaact ggacgatgaa cggcgagtac ggcagcgagt ttggcggatt
     3181 tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt tttcacctgg cgttatgttc
     3241 accgggcgat gtctctcagg tctgggtgct ggttctggtg aatgccggtg gtgagccgtt
     3301 tgcggtggtt caggtgcagc ggcggtttgc accggaagcc gtcagccatt cactggcact
     3361 ggcggcgtca ctggatgcgc aggggtacag cgttaatgac atcatccata tcctgatggc
     3421 ggaaggaggt caggcatgag cgtacattca cgggcactgg ttcccctctg cacagagcaa
     3481 caggccgcat ggcgggcggt ggcggagacg gaaaaacgcc gccatcaggg aaacacgctg
     3541 gcggaatatc cgtatgcggg ggcgtttttc cgctgtctga acggaagccg ccggatttcg
     3601 ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag aagaactgca cggcaaccgt
     3661 cttcagtggc tgtatgccat cgatgtgctg attgaaacac agggcgaggt ctgcctcctt
     3721 ccccttcccg acgatgcagc agaacgcttg ttcccgtcgg tgcggtttcg tgtcagggaa
     3781 cgcagtcgcc ataaatccgc actggtcatg cagaagtaca gccgtcagca ggcgagggag
     3841 gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc aggctgaaat cgaactggca
     3901 ttccattcgc cggaaacagt cggaagctgg catgcacgct ggagtgaccg ggtggcagag
     3961 catgacctgg aaaccctgtt ctggcagtgg ggcgagcgtt ttccgtcact gaccgggatg
     4021 gaacgctggc agtggcagga tatgccattc tggcaggtca tcgcagaggc tggtatggcg
     4081 gcaagggagg ccggtcacgc tgtccgtgag atggagcggt ggatggtccc gaacaaactc
     4141 cgggaggtgg cgtgatgcag aacgccagac acccgaaaac acacagggaa ctgtgtgaac
     4201 tggcctgccg ttttctccgc aacaacggct ttaaggtggt gttcagtgac cgttttcacg
     4261 catggacgtc ctgcggtgaa catcctgatg ccctgggctt ccgtaacgga gcgtcatgtc
     4321 tcattgaggt gaaatgctca cggtctgatt tcctggctga ccggaaaaag gtgttccggt
     4381 cagagccgga aaagggcatg ggagactggc gtttttatct ctgcgagccg ggggtgatta
     4441 ccgtggccga tttaccggcg ggctgggggt tgctgtatgc gattaacggg cgggtcagga
     4501 aagtacatgg ctggcccgga aatcatcagt gggtggttac ggtcgcgaaa ccgttcgggg
     4561 caaacaaaca ggctgaatgc gattatctgt tcagtgctct gcgtcggctg gaaatccggg
     4621 gcgtgacgga gaacggtgca gatgctgaca gggtgcagac atatgggcta tgatgtgccc
     4681 ggcgcttgag gctttttgcc tcatgacgtg aaggtggttt gttaccgtgt tgtgtggcag
     4741 aaagcagaaa gccccgtagt taattttcat taacccacga ggcccactgt atgtctaacc
     4801 aacaccagta tggcctctta ccgtgcaaat tgcaaggagg agtaaaccat gaaactaccg
     4861 ggagacgccc ttatctggtg cgtattaatc gtgtgctgca cgttgttgat attcacactc
     4921 ctgacccgga atcgcctgtg cgaagtccgg ctgaaggacg gatacaggga agttacggca
     4981 acaatggctt acgaatccgg cggtaagtag caacctggag gcgggcgcag gcccgccttt
     5041 tcagggctga tgctggtctg actgcactga agcgccttta taaaggggct gctggttcgc
     5101 cggtggcccc tttctccttg ctggcattgt gcgggcatga acaaccagac tgccaggaag
     5161 gccgtttccc tccgggaacg gggaagaacg cgcgcctgcg gcgcccgttc ttttcccccg
     5221 ccttctctgt ttatgacctg tcagaaatca catcccttcc ctttgattat tctctttttc
     5281 ctcctgttct gactctgatt actgctgccc tgattattgt ctgttttgtt gttctcctgg
     5341 ctgtgtcttt tccgggcgtc cccctggcgg gcttccatcc ctgacgcgaa accccgggct
     5401 tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cctggcatcc
     5461 cggcaaaagg ccgccgggat gtaaggcgca acgccgtttc gcaaaccgcg cttcacgtcg
     5521 ttgcggcggg tccctgcccg ttgttctgtc cggtgccatt cacctgttta agcccctgtc
     5581 gccgggggca tagacagcca caacctgccg cactgactgt ccagggtaaa tgaacagccc
     5641 acaaaatttt ttacctaaaa cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt
     5701 caggtcgtgg cagtgatgcc ccttgcggcg aaggttctca aaaaggtgat ggtgaatacc
     5761 ggagggaata aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaaa
     5821 gatgatgggg gcaaaaaaca gaggtgtatt aaagatgggc agaaatatgt cagcagcacg
     5881 taccgatggc tttattcgta atattcattc ccgcaatccc tttgatgtta tccgggctga
     5941 tgtggtgatc agcaggctgg aaaagcaggc tcattgggga tgtggtctgc actatgaaat
     6001 ttatgaagcg aatttgtttg atatggcgat gaatcatctt tcccggcttc ctctgaagga
     6061 tcgtccggta ttcattggtg ctgctgcacg aaaggggctt tttctgacga tggaagaaac
     6121 caggctggca cgggaagcga aagatgctct gatggctgaa cttgctctgg aatactgaaa
     6181 atcgggccgc accgtcgtcc cggatagtgc tgaaatattt aatgtggaga gaatggatgg
     6241 gatatatcac atatgttaca gatcaacgac ccggtgaacc agatatcctg accgggaaca
     6301 cgtttgctga tctggagatt tgtgattctg atgggcatct tctgctgaaa gtgtctgcac
     6361 ctgaagccgg ctggacacac gaaagcctga accttgttca gccgcaggaa gtgcaggagg
     6421 ggaatgatgc atttgatgct tatctgaatg gtatatggat cggttcgaca gaggtctgaa
     6481 caaatcacgc cagcagaaga cgttgctggc gtcttctgca ttcccgggga gctgtcgcgc
     6541 ggcatgtttg cttatacccg tgccccgaga tgattatttg tctgcgggcc tgaagacgcc
     6601 gggcaggcgg gaggataaat gcccgtctgc ccgcgccggg aaaagacagc acgccggtgg
     6661 cgctgaaagg ggccgcgctt acgcgccgcc cctttcatta cgcggatgtt ctgctgtgtg
     6721 cttctgtctt ttcatgcctg tcatttgtct gttactgatg atgttgttgt t
//
LOCUS       DAZFDP010000217         6727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFDP010000217 DAZFDP010000000
VERSION     DAZFDP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..270)
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_004674"
     CDS             complement(52..270)
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4766391.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(342..641)
                     /locus_tag="ACHJ1G_004675"
     CDS             complement(342..641)
                     /locus_tag="ACHJ1G_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766392.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            977..1279
                     /locus_tag="ACHJ1G_004676"
     CDS             977..1279
                     /locus_tag="ACHJ1G_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4766393.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            1282..1641
                     /locus_tag="ACHJ1G_004677"
     CDS             1282..1641
                     /locus_tag="ACHJ1G_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766394.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(1688..2080)
                     /locus_tag="ACHJ1G_004678"
     CDS             complement(1688..2080)
                     /locus_tag="ACHJ1G_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766395.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            2207..2467
                     /locus_tag="ACHJ1G_004679"
     CDS             2207..2467
                     /locus_tag="ACHJ1G_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4766396.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            2464..2901
                     /locus_tag="ACHJ1G_004680"
     CDS             2464..2901
                     /locus_tag="ACHJ1G_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4766397.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2988..3998
                     /locus_tag="ACHJ1G_004681"
     CDS             2988..3998
                     /locus_tag="ACHJ1G_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO4766398.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3991..4452
                     /locus_tag="ACHJ1G_004682"
     CDS             3991..4452
                     /locus_tag="ACHJ1G_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4766399.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            4486..5211
                     /locus_tag="ACHJ1G_004683"
     CDS             4486..5211
                     /locus_tag="ACHJ1G_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4766400.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPKAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            5227..5619
                     /locus_tag="ACHJ1G_004684"
     CDS             5227..5619
                     /locus_tag="ACHJ1G_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4766401.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5616..5912
                     /locus_tag="ACHJ1G_004685"
     CDS             5616..5912
                     /locus_tag="ACHJ1G_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4766402.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            5909..6370
                     /locus_tag="ACHJ1G_004686"
     CDS             5909..6370
                     /locus_tag="ACHJ1G_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO4766403.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            6348..6704
                     /locus_tag="ACHJ1G_004687"
     CDS             6348..6704
                     /locus_tag="ACHJ1G_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766404.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT         1817 a         1460 c         1826 g         1624 t
ORIGIN      
        1 tcacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct attaacgcgg
       61 gtatgtaatc attctggcaa tgcttaatgc cgctgctttt tccagattgg tgatatcctg
      121 ctccagagcg gacagatttt cagcctgctt agccctggct tcattggccc atttcaggtc
      181 ctgcgctgcc ttaattttct ggtgcatcca ctcataaagt tcatcatcgg tatagtctgg
      241 cgcgatgatg acgggttctc gtttctgcat gtcggctcct tgtggttagc gctgcctgct
      301 tttaaccacg tcaggcgagg tggtatcctc tgaggggtct gttactcgag aggaaattgg
      361 ttatgaatac aatcaagttt tcttgcccag aatgtggtgg cgaagtcttt gacacatcct
      421 ttaaaccgca tggctctgac agtttcgcgg gagccatctg caaaaattgt ggtcaccttg
      481 taactgaaga tgagtcctcg cagtttgatg acgaaatcgt tgacaatatc ttcggtgcac
      541 tcaccagaga ctttctgaag taaaggcgca taccgcttag ttaccgctct gataattctt
      601 acctgtccgg caatggcgct gatatcaata taaagcgcca tcgctgtttc tttgctgatc
      661 cctggatgcc ttccattctg atgtttgact tcgcccactg agaaatcctc tgtttcccct
      721 taacgccggg gtagcggaac aaaaaacctg ctgcatagtt aaacgtaatc cccgccgtca
      781 tgttcatacg cctcaggctg gctacttaac ccctgaccac tgccgggtaa ctcgaagtat
      841 tgccctgcgt tctgtggggc ggggtgggtt tatggataca atttacaaat taaaatttaa
      901 ctggtcaaca tgtttattat taaatttgta actgcgggct gttgcatcag tcccaaaatg
      961 ggactactat acagttatga agattatctc agttaaaaca ctcagggatt tttgggcgga
     1021 gaatcctgat gcagaacaac cgctaagggc atgggtggat gaggcggcaa aagctgactg
     1081 gaaaagccca gcagacatta aggcacaata ccgaacggcc agtatattga aaaaccggcg
     1141 cgtggtattc aacataaaag gtaatcatta ccgtttaatt gttgcaattg cgtatcagcg
     1201 aggatgggta tttgttaaat tcatcggaaa ccacaaggaa tacgatgcta ttgatgctga
     1261 taccatcgaa ctggagtaag catgaacatc aaacctattc gtacagagca agattatgaa
     1321 gccgcgttgc gtgctgttga acccatgttc gacaatgagc ccgaaattga cacgcctgag
     1381 ggggattttt ttgaggtgat gtgtttgcta atagaggaat atgaaaaaaa acattatccc
     1441 attgagccac catccccaat tgaagctata agatttcgca tggaacagca ggggctgact
     1501 gtgaaagatt tggaacccgc aattgggaaa aaaaatcggg tttatgaggt gttgaatggt
     1561 accagaagcc ttacgttacc aatgattcgc cgtcttcata atcaatttgg tatccccctg
     1621 gaaagcctgg ttggattata aaatctgcta gtcatttgcc tgatgctcgt tccagaaaag
     1681 gaatgcatca ggcagttttg tttttctgcc gcagtaactc ttcaagtttc cgtttataga
     1741 aatcgcgttt ttgctccata tcacgaatga tctgctctgc gtcgctttga ggtaactcat
     1801 ctaaaagcga tatgattttt cgttgttgtt ctgtaagttg cggttggttg tcattactgg
     1861 atacagccat tttatcgccg agagtttctt cttccataaa gaaccaatgg acgggatgtt
     1921 gtgagagctc tgctaatttt tccagtttat ccattcttgg catcacgcct ttcaaccaac
     1981 cttgcacgga ttggggtttt acaccaagac gtcttcccag ctctgactgg tttatattca
     2041 attcctgcaa cacctgctga aggcgtttta caaagatcat caccacccct cgtaaactag
     2101 ttccgcgatc ctacagaaaa aattgataag tggcattaca aatagaagtt gaaatttaaa
     2161 tttaaatttg taattatcgg tgtcatcgta aagttcggag ggaaacatgc aaaaaagtac
     2221 tcaagtgaaa atcctgtcaa taatgagcca atcagaatta gggcgtcgtc ttggtaaaac
     2281 accgcaaacc ataagtgggt ggtttaaaaa acgagtgcca gcggaggagg ttataccagc
     2341 atgtgaggcg cttgactggg gagtaactcc gcatgaattg cgccctgata aataccctaa
     2401 tccaaccgat ggtttacctg ttgagtatca ggctaacgca caagcagcgg cgggagttga
     2461 ttcatgaaaa tcaagcatga gcacatccgc atggcgatga atgcgtggct gctttatccg
     2521 agggtagggc gcaaaaaaat cgctgatgat atagcgacag catattttga gcttgaaatg
     2581 acttatccac caatgcatga cacctctacg acagagggta ttggattgaa catacaaaat
     2641 atttttcgct ggcttgaaaa ggatacgcct gatgctgttg aaaaaattca ggcactaatt
     2701 ccagctatcc tgactgttct tccgcgtgaa ctgcgttatc acctcagtat ttttgacact
     2761 gttgagcgcc gtgcattact ggcggcccag gaagcgttga gcacggcaat tgatgcgcat
     2821 gatgatgcag tccaggcagt ttaccggaaa gcgtatttca gcgacggcgg gtcatccggc
     2881 gagtctgttg tggtgcattg atatttatgc cagacccctg ctgattctgt tgattgggga
     2941 atcacagaat atcaccagag gatggttcgt cacaagatga ggcaattatg gccgcattac
     3001 catacatgca actgtacata gctgattacc tggctgacac catgcatttg tccgcagagg
     3061 agcacggtgc gtatttgttg ctgatgttca attactggca aacagggaaa ccaataccta
     3121 aaaacaggct ggcaaaaatt tcccgtctga ctaacgagcg atgggctgat gtggaaccat
     3181 ccttgcggga gtttttttgc gataacagcg atgaatgggt gcatcttcgg attgaggaag
     3241 atctggcatc agtcagggaa aaattgacca aaaaatcagc cgccggaaaa gcatctgttc
     3301 aggccagaag aagcagaaag gaagcatatg ttcaaacaaa acaagagaga gatttaacag
     3361 gtgttcaaac aaatgttggt gttgtgtttg aacatgacgc aaacacaaaa gcaactaata
     3421 aagatacaga tctaaaagaa ttaaacccca cacataacgt gcgtgaacgc gagagtattc
     3481 cgaccagtga gtcgcatggt gcgccgttgc agacagccga acctgaatac ctggacggcc
     3541 tgagcgaacc gatcgggaaa ttttcgatga ctactgtctg gcagccgtcg ccggattttc
     3601 gacaacgggc agcagtgtgg ggtatggctc tgcctgagcc ggaatttaca cctgctgagc
     3661 ttgccgcatt ccgggattac tggatggcgg aggggaaggt tttcacgcag gttcagtggg
     3721 agcagaaatt tgcccgccac gtgcagcacg tcagggcaca ggtaaaacca gtcagcaagg
     3781 gggtaagcca tgcagcatca ggtggcacgg catcacgggc agttcaggaa atccgggcag
     3841 cacgcgaaca gtgggaacgt gaaaacggat ttatcagcaa cggaaacggc ctggaagctg
     3901 tgggagctta tgggggaggt gtattcgaac cgctggactc agaagaacgg ggccgcacct
     3961 tcgaagctct ggattgccca gattggtgcg atgactgaac agcaaatccg tctggtctgc
     4021 cgtcagtgca tggaccgctg ccgggcgggt gaaacgtggc ccccggacct ggctgagttt
     4081 gttgcgctga tttcggagag tggggcaaat ccatttggtc ttacggtgga tgccgtgatg
     4141 gaagagtacc ggcgctggcg caatgaatcc tggcgatacg acgggagtga taaatacccg
     4201 tggccacagc ctgtgctgta ccacatctgc ctcgaaatgc gtaccagagg gattgagcgc
     4261 cagatgacgc agggtgagtt aaaactactt gcagaacggc aactgacgaa atgggcaaag
     4321 catgttggta acgggatgag tgttccgcca gtgcgacgac aactggaagg ggcgaaacac
     4381 ccgcaagggc caacgccaat tgagcggctg aaacaggaat acgaacgccg gaaggcagct
     4441 ggttttattt gaatctgaga aacgattttg tcggaggaaa ttttaatgga aaccgtattt
     4501 gacgcactga aagcactgaa aagagcctct tcacaggtag tggcggcccg ccttggaatc
     4561 agccgtgaag atgcggtcaa cgaactgtgg aaactgaagc gccgcggtga agcggataac
     4621 aaggggtcga tgtggtggct gattcaggct ggtgaaagtg aaccggtgtc accggtaccg
     4681 aaagtgacag cgcaaatgct gactgaggcg attgaacaac atggcccaca aacggcggat
     4741 gagctggcac tgatgttcgg gattacctcc cgccgggcga attcatcgct ggccatggta
     4801 atcagcaaag ggcgtctgat tcgcgtgaat cagggcggta aatttcgtta ctgcataccg
     4861 ggcgctgatt taccggcaga gccgaaagct gcatccgtag cggaaaccga tggtaaagcc
     4921 tttcctcagc cagcaggtgt tgcgttacca gttcgggaag cagaaacaca ggaagaaata
     4981 aaaacggaaa gtgtggcggt cacagtgcag tcacagccgt cgttcaccag aaaacatccg
     5041 gatggtctga ttttaccatc gctgcatgtg gctaaccgcg agctgcgccg ggcaaaaggt
     5101 caggttcaga agtgggagcg agtctgcgcc gcgctgcggg agctgaacaa gcaccgggat
     5161 attgttcgac agattactga ttcttcccgc cgtgttgtat cggaaaagtg attgccggag
     5221 gcgcttatgg caaaagaatt tacaccagaa gagcgggaag aagtgaaggc gcgcattgtg
     5281 gaattcgtgc gcctgagcgg acgagaaact tttcgacaac tggcagataa aacgggtgtc
     5341 agtaagaccg ctattcgtcg tttatctggt gcgcttgcgg ccagtggtga tgtctggctc
     5401 tctggttgcg gggtatttcc atcagagcag gcgtatcgcg tatggcgtaa gacaccggag
     5461 aaggctgctg acccgacact gattcgaaag ttacctgacg gagaaatacg tcgttacaac
     5521 agacggcaga acataatttg tcgtgagtgc cgccagagcg aagttatgca gcgtgtgctg
     5581 gcgttctatc ggggaaactt tcaggaggtg atggagtgag ggtgagagtt tatattgccg
     5641 gtccaatgac gggatatgaa aatttcaacc gtgaggcgtt tcacaaggcg gaagaggaac
     5701 tgaaacggga agggcatacc gtcttaactc cggcagtact tccggacggg ctgacacagc
     5761 cgcactacat ggatatttgc atggcaatga ttcgttgtgt ggatgcgatt tacatgctga
     5821 atggctggca gcggtcagcg ggcgctaagg cagagctggc actggcggag aaactggggc
     5881 atgcggtgat ttatcaggag gtggctcgat gagagaggtt aactatgagg cgcttcgtga
     5941 ggcagcacaa aactatcagt cgacgctggc gtggtatcag gctaccccgg acagcccaaa
     6001 tgctgaacgg gattgtgatg cggctcttgc tgcgtttaag cgtcatatcc gtcatcggga
     6061 agcggatatt atcgctggtt tgctggatgg actggaagaa gcaaaatcac aactcaacga
     6121 gcagcgtgag tattacgaag gcgttatctc tgatgggagc aagcgtattg ctgaactgga
     6181 tgcgcgggaa gttcaattac cgactcgcta cgaccttcga tatggacacc cgataaatgc
     6241 agatgagcga catgtcatga tacctaaaga aaatggcagt tggctttacc tgattgacct
     6301 agaacacgca ttacgcgtcg ctggcattcg catcaaagga gaggagcatg gaaataaaac
     6361 cagaggatga gttaagcaat atcgttttat ttccggtaaa agaggatgac cctcgtaatc
     6421 aggttaattt tctttatgag ccatcggaaa gaccatattg tcatcacgcc tctgttcggg
     6481 ttgacgaaaa agagcgccag gtccgctgta aaatctgcgg tgcggttgtg gagccgtttg
     6541 actggatgct ctctgtggcg aaaagagaaa ccagactggc agatgatgta aggctattgc
     6601 gccaggagga acaggaaagg cggaaaaata tagaaaagtt aattcagatt gaacgtaacg
     6661 cgaaagcgcg gatacgcagg gcgacaaaat ccagaactga ataaataaat ttagcgctgt
     6721 aaataaa
//
LOCUS       DAZFDP010000218         6723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFDP010000218 DAZFDP010000000
VERSION     DAZFDP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..269
                     /locus_tag="ACHJ1G_004688"
     CDS             <1..269
                     /locus_tag="ACHJ1G_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012967738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766405.1"
                     /translation="KMSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVT
                     EWPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            282..725
                     /locus_tag="ACHJ1G_004689"
     CDS             282..725
                     /locus_tag="ACHJ1G_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766406.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            1276..1482
                     /locus_tag="ACHJ1G_004690"
     CDS             1276..1482
                     /locus_tag="ACHJ1G_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO4766407.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            1558..1854
                     /locus_tag="ACHJ1G_004691"
     CDS             1558..1854
                     /locus_tag="ACHJ1G_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4766408.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1860..2645
                     /gene="bet"
                     /locus_tag="ACHJ1G_004692"
     CDS             1860..2645
                     /gene="bet"
                     /locus_tag="ACHJ1G_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4766409.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2642..3322
                     /locus_tag="ACHJ1G_004693"
     CDS             2642..3322
                     /locus_tag="ACHJ1G_004693"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4766410.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3319..3501
                     /locus_tag="ACHJ1G_004694"
     CDS             3319..3501
                     /locus_tag="ACHJ1G_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4766411.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            3474..3665
                     /locus_tag="ACHJ1G_004695"
     CDS             3474..3665
                     /locus_tag="ACHJ1G_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4766412.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            3742..3957
                     /locus_tag="ACHJ1G_004696"
     CDS             3742..3957
                     /locus_tag="ACHJ1G_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4766413.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4056..4274
                     /locus_tag="ACHJ1G_004697"
     CDS             4056..4274
                     /locus_tag="ACHJ1G_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4766414.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            4322..4600
                     /locus_tag="ACHJ1G_004698"
     CDS             4322..4600
                     /locus_tag="ACHJ1G_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766415.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            4572..4637
                     /locus_tag="ACHJ1G_004699"
                     /pseudo
     CDS             4572..4637
                     /locus_tag="ACHJ1G_004699"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4799..5962
                     /gene="intD"
                     /locus_tag="ACHJ1G_004700"
     CDS             4799..5962
                     /gene="intD"
                     /locus_tag="ACHJ1G_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO4766416.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(5978..6054)
                     /locus_tag="ACHJ1G_004701"
     tRNA            complement(5978..6054)
                     /locus_tag="ACHJ1G_004701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6018..6020),aa:Arg,seq:tct)
     gene            6297..>6723
                     /gene="fimZ"
                     /locus_tag="ACHJ1G_004702"
     CDS             6297..>6723
                     /gene="fimZ"
                     /locus_tag="ACHJ1G_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4766417.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCST"
BASE COUNT         1981 a         1558 c         1606 g         1578 t
ORIGIN      
        1 caaaaatgag taataaaaca cttgttaaaa aatcaaataa ctgtgtcgat gcatacgccg
       61 acgcgtttgg ctactcttcg tttggtgacg gctctcagcg cctaggttca atttcttttt
      121 ttcataatgt aactgaatgg cctgttgatg aaagtgacga tgttgagaac ataaagtaca
      181 acattgcaac cattagaatg cccgaagaat tgatgcttaa attggctgac ttcattcgcg
      241 accagcatga caaagctaaa acaaactaat cgtaattaat tatgaaacac gattattacg
      301 aagctccaaa cctaaatgat aaaattgcaa aggcggcaga atcattttgt ttgcactcca
      361 gtgcgtcttc ccagctagca ggtatatctg gaaaaagatg ggacatgcta caatcaagtg
      421 gtagcagtgg gtctgggagt gataacatgc ctgaaaaagt tgcaaaactg gagtccgatg
      481 ttgctcatat caaacgagac gttgatgaac ttaaaacaga cgttaaagct atcgacaaaa
      541 atatgataac tattcttgca cgtctggact ctataaaaga atccttaaca aacaagccat
      601 caagtgatgc tgtcgataga aagatttcag acgcaaagct tgcggtattg cttggtgttc
      661 cagcaatcat cgctgcagga acaggacttt ataagctatc aatgtacttt ttctttagtg
      721 cttaagaaaa tgctcgcacc cttgcattag gtaaagcgat ttttcattcc ccattaccct
      781 cgctcttaac caatcatcta aacccggcca ccgtgccggg ttttcttttg ccctcccctc
      841 atcatacacc gttcaaaaaa accaccacga cctcgcttca gttatcgcta tgcgatgcaa
      901 gtcacaaaat aaatccatct taaatacaac cagttatatc taaaacaacc aacaaaacaa
      961 cttttgttgt tgacgataaa acaactatag ttttaaatga gttcatcgca acaacacaac
     1021 gatacggcaa tcacctgatt caccgttgcg atgaccgctt agatccgcag cttgaatttc
     1081 agcaggcttc ggggagtgcg agggatgaaa cggacgcgtg aacgtcggtg tgaccagctg
     1141 aaatcaactc aacatttcat accttagtcg cttcaacgag gcggcttagt tatgacaacc
     1201 ggcggccatc caccgcctga atacgcgcag aagtctttat atgttcagca gcccagctta
     1261 cgggcaggag tttttatggt tcatcaacat tacggaacgc agaccgttaa tcgcggtgcg
     1321 gtcatgccag gaatgctggt caaacgcaaa gatggtacct ggactgcatc agctaattta
     1381 cgcggacgac tttatctgca tcgcggcatt gagcgcactt atacccgtga cttgctcgtg
     1441 gaagtttttc tcgacggacg cggcaacgct ctgaatcact aatccccttt cctgttttcc
     1501 gaatcagcct ggcattccgc gggcgatttt ttcacagcca ttttcaggag ttcagccatg
     1561 aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca ctaccagcag
     1621 atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg cctgccccag
     1681 cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag caaaaaagcc
     1741 attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga acacatccgg
     1801 tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt ataaaacgga
     1861 tgagtacagc actcgcaacg ctggcaggga agctggctga acgtgtcggc atggattctg
     1921 tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt gatgccagcg
     1981 atgcgcagtt catcgcattg ttgatcgtcg ccaaccagta cggccttaat ccgtggacga
     2041 aagaaattta cgccttccct gataagcaga acggcattgt tccggtggtg ggcgttgatg
     2101 gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt gagcaggaca
     2161 atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc tgcgttaccg
     2221 aatggatgga tgaatgccgc cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg
     2281 ggccgtggca gtcgcatccc aaacggatgt tacggcataa agccatgatt cagtgtgccc
     2341 gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc attgtcgaaa
     2401 atactgcata cactgcagaa cgtcagccag aacgcgacat cactccggtt aacgatgaaa
     2461 ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     2521 tgccgctctg ttcccagata tttcgccgcg acattcgcgc atcgtcagaa ctgacacagg
     2581 ccgaagcagt gaaagctctt ggattcctga aacagaaagc cactgagcag aaggtggcag
     2641 catgataccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg
     2701 ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt
     2761 gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct acttccacac
     2821 cctgctggct gaggtttgca ccggtgtggc tccggaagtt aatgctaagg cgctggcctg
     2881 gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg gcgtgaatat
     2941 tactgaatcc ccgatcatct atcgcgacga aagtatgcgc accgcctgct ctcccgatgg
     3001 tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc gggatttcat
     3061 gaaattccgg ctcggtggtt tcgaggcaat aaaatcggct tacatggccc aggtgcagta
     3121 cagcatgtgg gtgacgcgaa aagatgcctg gtactttgcc aactatgacc cgcgcatgaa
     3181 gcgtgaaggc ctgcattatg tcgtgattga gcggaatgaa aagtacatgg cgagttttga
     3241 cgagatggtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt
     3301 atttggggag caatggcgat gacgcatcct cacgataata tccgggtagg cgcgatcact
     3361 ttcgtctact ccgttacaaa gcgaggctgg gtatttcccg gcctttctgt tatcagaaat
     3421 ccactgaaag cacagcggct ggctgagaag ataaataata aacgggaggc ggtatgcaca
     3481 aagcatctcc tgttgagtta agaacgagta ttgagatggc acatagcctt gctcaaattg
     3541 gagtcaggtt tgtgccaata ccagtagaaa cagacgaaga atttcatacg ttagccacat
     3601 ccctttcaca aaagctggaa atgatggtgg cgaaagcaga agcagatgag agagaccagg
     3661 tatgacaacc actgaatgca tttttctggc agcgggcttc atattctgtg tgcttatgct
     3721 tgccgacatg gggcttgttc aatgacacct cagcaagaaa acgcccttcg cagcattgcc
     3781 cgtcaggcta attctgaaat caaaaaagcc agacagcatt ttccggataa aaacgtcgat
     3841 gacatttgcc gtagcgtact aaagaagcac cgcgaaacgg taacgctgat gggattcaca
     3901 ccgactcatt taagcctggc gatcggcatg ttaaacggcg tctttaagga acggtgaaca
     3961 tgaaaagcaa aatcatcagg gagctacagg ctcctttttt attgttcgca ttcatcctca
     4021 agcgtattaa ccaacaattc agggattaat ggaagatggc agacatcatt gattcagcat
     4081 cagaaatcga agaattacag cgcaatacag caataaaaat gcgtcgtcag aactaccaga
     4141 ctgtatccgc aactcattgt tgtgagtgtg gcgatccgat agatgagcga agacgcctgg
     4201 ctgttcaggg ttgtcggact tgtgcaagtt gccaggagga gatcgaactt aagaacaaac
     4261 aatggggact gtgatggcct caaagcagca aatttcaaca tcgtccaact gaggtgtaaa
     4321 aatgttcaga atcatttttc ctaacacctg gtacgtcgac caccacggca ctccctgcaa
     4381 aatcctgcgt tctacccaca acaaagttca ctacatccga aaaggcagaa catgtatcgc
     4441 cagcatgttc cgctttaatc atgactttga acctgtgaat aaagctgatg cagatcggat
     4501 agcagaagag atcgaaacgg cagaacacat taagaagtta cgtgacatgc gttcaaaaag
     4561 cagaggtaac catggaatca tacagcctca cactcgatga ggcctgtcag tttcttaaga
     4621 tatccagacc aaccgccacc aactggatac gaacaggccg cctacaggca acacgtaaag
     4681 atccaaccaa gccaaaatct ccttacctca caacacggca agcctgcatt gcggcgcttc
     4741 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
     4801 gtcactcttc cgcagaaatg aaatatggta tgcctcgtat tcgctcccgg gcgggaaacg
     4861 aattaaggaa tctcttggca caaaggacaa gcggcaagct caggagttgc acgacaagcg
     4921 aaaagcagaa ctctggcgag tagaaaagct aggggattta cctgatgtca cttttgaaga
     4981 ggcctgccta agatggcttg aggaaaaagc tgataaaaaa tctctcgatt cagataaaag
     5041 ccggattgag ttctggcttg aacattttga gggtataagg cttaaagata tctcggaggc
     5101 aaagatttac tctgctgtaa gcagaatgca taacagaaag acgaaagaaa tatggaaaca
     5161 gaaagttcag gccgccatca ggaaaggtaa agaaccgcct gtttatgaac caaagccagt
     5221 atcaactcag acaaaggcaa agcatcttgc catgataaag gccattctcc gtgctgcaga
     5281 acgcgactgg aagtggctgg aaaaagcgcc tgtcatcaag ataccagcgg tcagaaacaa
     5341 gcgagtcaga tggctggaaa aggaggaagc aaaacgcctt attgatgagt gccccgaacc
     5401 actgaaatct gtcgtcaagt ttgcgctggc aactggtctg agaaagtcga acatcataaa
     5461 tctggaatgg caacaaatcg acatgcagcg acgagttgcc tgggtgaatc cagaagagag
     5521 caaatcaaac cgcgccattg gtgtggcgct gaacgatacc gcctgtaaag tgttgcgtga
     5581 tcaaataggc aagcatcaca aatgggtgtt tgtacatacc aaggcggcta agcgagcaga
     5641 tggaacatca acgcctgcgg tcaggaagat gcgcatcgac agcaagacat catggctatc
     5701 agcttgtcgt cgtgcaggaa ttgaagattt ccgtttccat gacctcagac acacctgggc
     5761 aagctggctg attcagtcag gcgtcccatt atcagtgctt caggaaatgg gcggatggga
     5821 gtccatagaa atggttcgta ggtatgctca ccttgcgcct aatcatttga cagagcatgc
     5881 gaggaaaata gacgacattt ttggtgataa tgtcccaaat atgtcccact ctggaattat
     5941 ggaggatata aagaaggcgt aactgattga attgtaatgg cgcgccctgc aggattcgaa
     6001 cctgcggccc acgacttaga aggtcgttgc tctatccaac tgagctaagg gcgcgttgat
     6061 accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc cttgctgagt caatggcttt
     6121 tgatctggtt gctgaacaag tgaacgaccg cgtctgattt tctgatttat ttcgctatag
     6181 cggcaaacaa acacacaccg ctgcgcgtct gaatcaagaa aacccgtatt ttcatgtatc
     6241 aaagtacaat ttaccgccct aacggaaaat tgtccgctcc tatgagactg gtaactatga
     6301 aaccaacgtc ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc
     6361 tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca
     6421 tcgattatct ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa
     6481 cagacggttt taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat
     6541 ttttatcatc gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg
     6601 gttttgtcag taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct
     6661 ccggatacac gttttttccc agcgaaacgc ttaactatat aaaaagcaat aaatgtagta
     6721 cga
//
LOCUS       DAZFDP010000219         6710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFDP010000219 DAZFDP010000000
VERSION     DAZFDP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..164
                     /gene="xisR"
                     /locus_tag="ACHJ1G_004703"
     CDS             <1..164
                     /gene="xisR"
                     /locus_tag="ACHJ1G_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4766418.1"
                     /translation="KKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQA
                     SARPRRHLT"
     gene            199..1479
                     /locus_tag="ACHJ1G_004704"
     CDS             199..1479
                     /locus_tag="ACHJ1G_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4766419.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHJ1G_004705"
     CDS             complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHJ1G_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO4766420.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHJ1G_004706"
     CDS             complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHJ1G_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO4766421.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHJ1G_004707"
     CDS             complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHJ1G_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4766422.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHJ1G_004708"
     CDS             4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHJ1G_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4766423.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4955..5515
                     /gene="speG"
                     /locus_tag="ACHJ1G_004709"
     CDS             4955..5515
                     /gene="speG"
                     /locus_tag="ACHJ1G_004709"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4766424.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHJ1G_004710"
     CDS             complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHJ1G_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4766425.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHJ1G_004711"
     CDS             6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHJ1G_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4766426.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1925 a         1622 c         1467 g         1696 t
ORIGIN      
        1 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
       61 acggtatgcc atgggataac agtccatgct tttataacct ggaagaaatt gatcgctgga
      121 ttgagcgcca ggcatcagcg agaccaagac gtcatcttac ttgactaaaa gccacactaa
      181 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
      241 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa atctgggggt tcctgacaca
      301 tcaaaaaaca ggcgcgttgc aggtgaacta cgctcctctg tttgttacgc aataaaaact
      361 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
      421 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttttggcact gaaagaaact
      481 gaagtcgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
      541 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
      601 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
      661 tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
      721 gataacggtt acctggcaga taatccgttt aaggggatct cgccattaaa ggaatcaaga
      781 accattccgg atcctctttc gcgggaggaa tttatccgtc ttatcgatgc gtgcagaaat
      841 cagcaagcaa aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
      901 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
      961 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     1021 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     1081 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     1141 tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     1201 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     1261 tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc taacccggca
     1321 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     1381 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcaagttt
     1441 gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     1501 aacacgcatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     1561 ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata atggttatct
     1621 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     1681 tgagcaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     1741 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     1801 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     1861 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     1921 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     1981 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     2041 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     2101 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     2161 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     2221 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     2281 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     2341 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     2401 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgc tcaccgactc
     2461 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
     2521 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
     2581 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
     2641 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
     2701 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     2761 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     2821 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
     2881 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     2941 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
     3001 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     3061 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
     3121 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     3181 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     3241 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     3301 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     3361 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     3421 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     3481 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
     3541 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     3601 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     3661 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     3721 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     3781 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     3841 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     3901 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     3961 gagctactac catacaagca taaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
     4021 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
     4081 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
     4141 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
     4201 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
     4261 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
     4321 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     4381 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     4441 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
     4501 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
     4561 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
     4621 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
     4681 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
     4741 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
     4801 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
     4861 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
     4921 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
     4981 acgcccgctg gagcgtgaag atttacgcta tgaacatcaa ctcgacaaca acgccagtgt
     5041 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     5101 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
     5161 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
     5221 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
     5281 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
     5341 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
     5401 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
     5461 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
     5521 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
     5581 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
     5641 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
     5701 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
     5761 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
     5821 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
     5881 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
     5941 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
     6001 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
     6061 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
     6121 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
     6181 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
     6241 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
     6301 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
     6361 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
     6421 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
     6481 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
     6541 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
     6601 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
     6661 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat
//
LOCUS       DAZFDP010000220         6645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFDP010000220 DAZFDP010000000
VERSION     DAZFDP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..239)
                     /locus_tag="ACHJ1G_004712"
     CDS             complement(<1..239)
                     /locus_tag="ACHJ1G_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO4766427.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLL"
     gene            797..952
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004713"
     CDS             797..952
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4766428.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(994..1173)
                     /locus_tag="ACHJ1G_004714"
     CDS             complement(994..1173)
                     /locus_tag="ACHJ1G_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766429.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            1120..1392
                     /locus_tag="ACHJ1G_004715"
     CDS             1120..1392
                     /locus_tag="ACHJ1G_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766430.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1394..2449
                     /locus_tag="ACHJ1G_004716"
     CDS             1394..2449
                     /locus_tag="ACHJ1G_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766431.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            2450..2815
                     /locus_tag="ACHJ1G_004717"
     CDS             2450..2815
                     /locus_tag="ACHJ1G_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4766432.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            2824..3366
                     /locus_tag="ACHJ1G_004718"
     CDS             2824..3366
                     /locus_tag="ACHJ1G_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4766433.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            3598..3795
                     /locus_tag="ACHJ1G_004719"
     CDS             3598..3795
                     /locus_tag="ACHJ1G_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4766434.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            3946..4995
                     /locus_tag="ACHJ1G_004720"
     CDS             3946..4995
                     /locus_tag="ACHJ1G_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4766435.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSGLNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            5045..5120
                     /locus_tag="ACHJ1G_004721"
     tRNA            5045..5120
                     /locus_tag="ACHJ1G_004721"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5078..5080,aa:Ile,seq:cat)
     gene            5218..5294
                     /locus_tag="ACHJ1G_004722"
     tRNA            5218..5294
                     /locus_tag="ACHJ1G_004722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5252..5254,aa:Arg,seq:tct)
     gene            5839..5925
                     /locus_tag="ACHJ1G_004723"
     CDS             5839..5925
                     /locus_tag="ACHJ1G_004723"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4766436.1"
                     /translation="MSVLADCVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(6019..6135)
                     /locus_tag="ACHJ1G_004724"
                     /pseudo
     CDS             complement(6019..6135)
                     /locus_tag="ACHJ1G_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6206..6541)
                     /locus_tag="ACHJ1G_004725"
     CDS             complement(6206..6541)
                     /locus_tag="ACHJ1G_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4766437.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT         1769 a         1415 c         1717 g         1744 t
ORIGIN      
        1 attaataatc catcaatata aacaccttgc atatctgagc aagattttga gactttcttt
       61 ccgaactcta taagagtctc gttataactc tttaaggcaa tacctgaatc agggtcaatt
      121 ggcactggtt caatttcgag taatggcaaa atttttgatt tcttttcaat ggatagctgc
      181 gataaagctg ataactcaga acgtttcgct ttcagaatag gaatatatga aattgtcatg
      241 ttaatacctt atacagaatc aattaacaat taatggttct tcggacattg aaatttatat
      301 cgcaaaaatt ggcaaactct cctcgactac tcacttgtgg gtatcctggc gtaaaaggaa
      361 tgacttggca atacccggtg tgatcataag tcattgaaaa tgatcatttt tatcagtctt
      421 tcttccatga tgaatgctaa tgccatttga tttgttgagg tgaaaactgt taaaaatcaa
      481 aacgatgtaa ttgaaatgaa cgttcggtag cattcacgct ttaaatgttt cttttgtgct
      541 gattggatga atttgggtca cttctgatga gagatgttgc aggaaaagaa gttggcattg
      601 atctattgga tagttagaat tgctgcgggt gcttgaggct atctgcctca ggcatgaaca
      661 ccaaaaggca gatagagaaa agccccagtt aacattacgc gtccggcaag acgcttaaca
      721 ttaatctgag gccaatttca tgctttgcac atgtaggtta gcctcttacg tgccgaaagg
      781 caaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg
      841 tttaaccgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacgaatccg
      901 aaccggccag acggaggtcg ctgtcttcac agcttacgaa cctgaggagt aagagaccag
      961 gcgagggaga aatccctcgc cacctctgat gagtcaggca tcctcaacgc acccgcactt
     1021 aacccgcttc ggcgggtttt gttttttcct ggcattctgg tttacaattc gcacgtcagc
     1081 ctgaacacct gacacctgct gcgccagcag agaaaacaga tggtgcacaa aaccaaattt
     1141 cacaattctg ataccgacct tgccatccgg catgggcggc gttcacacgc atttaaaacc
     1201 gactggtacc aacacccacc atgtactgaa gaacaggccg aatggctaat tcataactac
     1261 cgcagacgcg gatacgagat taagaaagcc ctcagcctcg attatcgtca ctggataatc
     1321 tatgtcaggc tcccttattc cgaacgccca ccgcgtccgt cccgcacatt ccagcaacgc
     1381 atctggaggt aacgtgcggg tattacttcg acctgttccg gtaccggaac ttgggctggt
     1441 ggtgctaaaa ccgggccgtg aatccatgca ggtatttcat aaccctcgag ttctggtgga
     1501 gccggaaccg aaaagcatgc gcggtctgcc gtccggagtc gttcctgctg ttcgccagcc
     1561 gctggcggag gataaatcat tactgccatt tttcagcgat gagcgggtga ttcgtgctgc
     1621 tggtggtgct ggtgcattgt ctgactggct gttacgccac attaaatcct gccagtggcc
     1681 tcatggtgac taccatcaca gtgaaaccgt catacatcgt tacggtaccg gcgcgatggt
     1741 gttgtgctgg cactgcgaca accagctgcg taaccagact tccgaatcac tcgggcagct
     1801 tgctcaccaa aacctgtcag catggatgat tgatgtcatc cgccacgcaa tgaatggcac
     1861 gcaggagcgg gaattatcgc tggctgaatt atcctggtgg gcaaccataa ataacgtagc
     1921 ggacgcacta ccggagacgg tgttacgtcg ttcactggga ttacgcgcgg aaaaaattcg
     1981 ctcagtatac cgcgagagcg acatcgtgcc gggagagcag accgccacca gcatgctgaa
     2041 gcagcgcaca aaaaatattg cgctactgcc tcacgcccac cagcaaaacc cgccacagga
     2101 aaagacggtg gtaagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca
     2161 gaaaccacga cgggaagaga tgcctgtata cacgcgttgg gtaaaaacgc agaaatgcat
     2221 gacgtgtggt aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat
     2281 gggaacaaag gctgatgatt tgtttgttat tccgctgtgc cgtaaatgcc atagcgaact
     2341 acacgccggg gtaaaagatt ttgaagaaaa acacggcagc cagctgttgt tgctgattcg
     2401 ttttttaatg cacgcgagaa attcgggtgt cctgaagtgg aaagcatgaa tgactgaacg
     2461 catagaattt gttttgcctt acccgccaac ggtgaacact tactggcgac gtcgtggcag
     2521 cacatatttt gtatcaaaag ccggtgagcg ttatcgccgt gatgtggcgc ttattgttcg
     2581 ccagcagcgg ctgaaattaa acctgtccgg aaggctggcg ataaagatta ttgcagagcc
     2641 accggataaa cgtcgtcgtg acctggacaa tatcctgaaa gcaccactgg atgcgctgac
     2701 gcatgccgga cttctcatag acgacgagca gtttgatgaa atcaatattg tgcgcggtca
     2761 gcgcgttcct gggggacggc tgggcgtgaa gatttacaaa attgagagtg agtgatcgta
     2821 aatatgatat acccggaaat tacaggcaaa agcggcgagc atttacgtct aaaaacgctg
     2881 gaagccgtct ggatccaggg gaaattacgg atgtgggggc gttggtcgta tataggtggt
     2941 ggcaaaccag gaaatatgtt caatcagttg ctggcatcca aaaaactgac aaaaaccgca
     3001 atcaatgaag ccctgcgtag aatcagggag tcagggattg ataaaccaga gctggaagca
     3061 ttcttgcgag agatgatcgc tggcagacag aagagctggt tgtctcactg tactgatgca
     3121 gaggcgttac gcattgatgg ggtgataagt aaagcgcttg cacgttatcc tggattgatt
     3181 gatatcctgc ggcaaaggta cgaagggcgg gggatgagta aacgcaaaat ggctgaattg
     3241 ttgaatgagg tgcacccgga atggtgtttt agtacatgcg aaaagcgaat tgctaattgg
     3301 ttggctgttg ctgaatatgc gctatacatc cctatgcgag aatcgtttgc tcaaaaaatg
     3361 tcttgatttt ttacgcataa actgtttcaa tccagctacg cttcgcaaag ctataccgcg
     3421 aggcgaatag cagacatgga cacctgaaag aacccgcttt atgcgggttt ttttatgtct
     3481 gaaaaacggc acagaacatt aaacgcgctg gtagttgtga atactggtct ttcagcttgc
     3541 tggctttttc gacaagaggt attggtatgt cacgttaacc ggaaaaggga aaaaagcatg
     3601 ctaaaacagc aggatatgac cgaaaccgcc agagtggtgt ttaatgaatt aagcgtcacc
     3661 gaaccggcga ccgtcgggga aattgcgcag aatacttacc tttcacgcga acgctgccag
     3721 ttaatactga ctcagcttgt tatggcgggt ctggcagatt atcagttcgg ttgttacaga
     3781 cgccttccgc agtgaaggct ttttaatttg tggtaatggg cggctggtgg gtgttagcgg
     3841 cacctgccag ccatctgctc atgcgttggg gtcacaagca aacctcaggc ccatctgctt
     3901 tgcgcaaaag cggtatgagc ctatcagaga agtgcttatt gatctatgat taatactgta
     3961 aaaatatcca gttgtgagtt aatcaacgct gattgcctgg aatttatcca gaccttaccg
     4021 gaaaactctg tcgatctgat agtcacagac ccgccatact ttaaagtgaa gcccgagggc
     4081 tgggataacc agtgggaggg cgacgatgat tacctgaaat ggctggacca gtgtctggcg
     4141 cagttctggc gggtactgaa gcctgccgga agtctttacc tgttctttgg tcatcgcctg
     4201 gcatctgaca ccgaaatcat gatgcgtgag cgctttaatg tgctgaacca tattatctgg
     4261 gcgaagccgt ccggacgctg gaacgggtgc aataaggaaa gtctgcgggc gtattttccg
     4321 gcaacagagc gcattctgtt tgcagaacat tatcagggac cgtatcgccc gaaagatgat
     4381 ggctatgtgg cacaggggcg cgagctaaaa cagcacgtca tggccccgct gatttcttac
     4441 tttcgtgatg cgcgtaaatc actgggaata acgtcaaaac agatagcgga agccaccgga
     4501 aagaaaaaca tggcttcgca ctggtttggt accagtcagt ggcagttacc gaacgagggt
     4561 aattacaaca aattgcaggc gttgtttgcg cgtgttgcgg cagaaaaaca tcagcgcggg
     4621 gaactggaaa agccacacca ccagctggtc agcacataca gtgggctgaa ccggcagtat
     4681 acggaactgc tgagtgaata taaaaatttg cggcggtatt tcggtgtgac ggcgcaggtt
     4741 ccgtacaccg atgtctggac gcataaaccg gtgcagtact atccagggaa acatccgtgc
     4801 gaaaaaccgg cagaaatgct gcagcagata atcaacgcga gcagtcgtcc gggagacctg
     4861 gttgcagatt tttttatggg ttcaggttca acggtaaaag cggcgatggc actggggcgt
     4921 tgtgcgattg gtgttgagct ggagacagga cgttttgaac agacagtcag ggaagttcag
     4981 gatttaatcg tttgaaacgg atgggattgc agaattaatt acgcaccatt attattctgc
     5041 taccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg tcgctggttc
     5101 aaatccagca agggccacca tcacataccg ccattagctc atcggcagag agcgtcagct
     5161 ttcgaaactg gctgtgtggg gcccgggtcc ccgatggcaa tccattatct gcattatgcg
     5221 ttgttagctc agccggacag agcaattgcc ttctaagcaa tcggtcactg gttcgaatcc
     5281 agtacaacgc gccatattca ttcttccaga tttcttccgg cagagcctta tactggaata
     5341 tacctggctc aggatattgt tgaaaatatt atatgtttgt caaaaataaa agttctgtta
     5401 agtattgatt gagtgtttgt tatacggtct aatggttttt tcagtattaa atatttatca
     5461 ttcatatggt gtgggtagag tgaatattga tgaggcgtcg gggtgtttca tccttaggca
     5521 gcgtattgat atagtcaatg cagcacgagc aaaggccttc agccgtttga cagttttgtt
     5581 ctgtactcct gatcgtcttt cgggaagaga cgttattctt ctgaatagtg atgctataca
     5641 gagggtttgc gatgagttca tggttgctaa ttcagaatta tttgctcttg ttcaggagta
     5701 caacagaata gccaggacct gtggtatgga tgaacttcgg attactcatc tggggtagat
     5761 acatatctgg attatcacct gttacggtaa aaagtgattg cttactgttt ttgtgaatgg
     5821 cattgcagca gccggataat gtcagtgctg gctgactgtg tactggtggc gggtgtggtg
     5881 gttgttgctt tcccgttgct gaaaaagaaa acgccagact gttagccggg tatcagttag
     5941 cgggagaaat ttttaaatac ttcacaattc aggcggttga ctgttgtctg gtttgcgggg
     6001 agtttgttaa aaaaaactgg catggtgaat ccccctgtgc ggaggggcaa tcagcgagta
     6061 ggtatatggg ataatcgcgg attcaggtgc tggtactgaa ttcaccggga ggcacccggc
     6121 accatgcaat ggcacatagc gccactctcc agcccctctc cggaggggct gtttatattg
     6181 attttgtcag atgtgagtaa actccttatg gactttgttg ttttagtcca taaggacata
     6241 tttgcagagt gcaacggtta ttaaagcatt cattcaatac gttatctgta tttgtagggc
     6301 attcctggct gtttttgatt aaattccaga atgttttatt gaatggtact acgttgtaaa
     6361 tggttacagg tagcactttg ttattgagca tgatgcctgt gtgagtcagt gtaaatatac
     6421 tttcaggagg taagaaagca tccgattgat accagattat taattttatt ttactccata
     6481 tgactgaaaa agatattccg catgatggct ggataactgt atcaatcaca atccacttca
     6541 tttactttcc ttgtttatgc cttgctggtg atgttctgaa aagtataaat gatatttttg
     6601 aattaaacca tagagcagaa ttatttttct gatgttgttt attgt
//
LOCUS       DAZFDP010000221         6608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFDP010000221 DAZFDP010000000
VERSION     DAZFDP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..146)
                     /locus_tag="ACHJ1G_004726"
     CDS             complement(<1..146)
                     /locus_tag="ACHJ1G_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766438.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSL"
     gene            224..1318
                     /gene="ddlA"
                     /locus_tag="ACHJ1G_004727"
     CDS             224..1318
                     /gene="ddlA"
                     /locus_tag="ACHJ1G_004727"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4766439.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1342..1554)
                     /gene="yaiZ"
                     /locus_tag="ACHJ1G_004728"
     CDS             complement(1342..1554)
                     /gene="yaiZ"
                     /locus_tag="ACHJ1G_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4766440.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1814..2122
                     /gene="yaiY"
                     /locus_tag="ACHJ1G_004729"
     CDS             1814..2122
                     /gene="yaiY"
                     /locus_tag="ACHJ1G_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4766441.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHJ1G_004730"
     CDS             complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHJ1G_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4766442.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHJ1G_004731"
     CDS             complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHJ1G_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4766443.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4860..6017
                     /gene="ampH"
                     /locus_tag="ACHJ1G_004732"
     CDS             4860..6017
                     /gene="ampH"
                     /locus_tag="ACHJ1G_004732"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4766444.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6018..>6608)
                     /gene="iprA"
                     /locus_tag="ACHJ1G_004733"
     CDS             complement(6018..>6608)
                     /gene="iprA"
                     /locus_tag="ACHJ1G_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4766445.1"
                     /translation="GKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDTFVILEGVISLR
                     REENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQTITLIEQNQLWR
                     DAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMNYIHQRTR
                     ISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1672 a         1694 c         1603 g         1639 t
ORIGIN      
        1 agagaaaaaa actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgtta cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gttcgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg ttaccaaacg
      661 tctgctgcgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgccaatcg
      721 tcacaacatt agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg aattcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaacccg caagccagca cctgtggcga
      961 gatcgtactc accagcgact tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc
     1081 cgttcaggct tatcaaacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     1141 cccagaaaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     1201 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     1261 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     1321 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     1381 aatcagcccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     1441 ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc
     1501 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     1561 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     1621 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     1681 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     1741 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     1801 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     1861 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     1921 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     1981 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     2041 cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca
     2101 ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct
     2161 ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     2221 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     2281 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     2341 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     2401 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     2461 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     2521 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     2581 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     2641 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     2701 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     2761 cgcacaggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     2821 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     2881 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     2941 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     3001 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     3061 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     3121 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     3181 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     3241 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     3301 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     3361 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     3421 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     3481 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     3541 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     3601 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     3661 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     3721 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     3781 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     3841 agcaccggca gaaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     3901 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     3961 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     4021 acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     4081 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     4141 agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact
     4201 tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc
     4261 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     4321 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     4381 ccggaagcgc cggtgatacg cgccacccag tcacccccac cggcttgcca gaagataacg
     4441 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     4501 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     4561 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     4621 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     4681 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     4741 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     4801 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     4861 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     4921 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     4981 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     5041 gcgtctttcg cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     5101 cggtcgtgcg tattgcttcc ctcaccaagc taatgaccag tgaaatgctg gtgaaattgc
     5161 tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     5221 gcgtgccaac ctacaatggg acgcccatta cgctggtcaa tctggcaacc cataccagcg
     5281 cgctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     5341 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     5401 cggcgtactc taaccttgcg ttcgacctgc tggctgatgc actggcgaat gcgtccggca
     5461 agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca
     5521 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgcagagcgc ggtgccagtc
     5581 catgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     5641 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     5701 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     5761 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggtcccg aaagagggtc
     5821 gtccggggat tattcagaag acaggaggtg gtggcggttt cattacctat atggcgatga
     5881 tcccgcagaa gaatattggc gcgtttgtgg ttgtcactcg ttcgccgttg acgcgcttta
     5941 aaaatatgag cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgctgg
     6001 ttatccccgc gtcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     6061 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     6121 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     6181 tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca
     6241 atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg
     6301 tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat
     6361 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     6421 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     6481 agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     6541 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     6601 agcttacc
//
LOCUS       DAZFDP010000222         6583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFDP010000222 DAZFDP010000000
VERSION     DAZFDP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            245..4498
                     /gene="fdeC"
                     /locus_tag="ACHJ1G_004734"
     CDS             245..4498
                     /gene="fdeC"
                     /locus_tag="ACHJ1G_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO4766446.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4619..5476)
                     /locus_tag="ACHJ1G_004735"
     CDS             complement(4619..5476)
                     /locus_tag="ACHJ1G_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766447.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5725..>6583
                     /locus_tag="ACHJ1G_004736"
     CDS             5725..>6583
                     /locus_tag="ACHJ1G_004736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO4766448.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVK"
BASE COUNT         1784 a         1474 c         1730 g         1595 t
ORIGIN      
        1 ctatttaatg acttgcacaa aaagctaaat tttcccccat aaataaaaat ataatcccgc
       61 gcccaaccac ctgatgagtg gctacaggca ctggatatat taggtggcgg tgcactttct
      121 tacataaagg tatttccttt tctgcggaaa aggaaatcgg gaaatccccg gtttttctga
      181 caagcagacg ccattatttg tgtctgccta tgttcgttaa ttcgttcatc aggaaattat
      241 ctcaatgtca cattataaaa caggtcataa acaaccacga tttcgttatt cagttctgac
      301 ccgctgcgtg gcgtgggcaa atatctctgt tcaggttctt tttccactcg ctgtcacctt
      361 taccccagta atggcggcac gtgcgcagca tgcggttcag ccacggttga gcatgggaaa
      421 tactacggta actgctgata ataacgtgga gaaaaatgtc gcgtcgtttg ccgcaaatgc
      481 cgggacattt ttaagcagtc agccagatag cgatgcgaca cgtaacttta ttaccggaat
      541 ggccacagct aaagctaacc aggaaataca ggagtggctc gggaaatatg gtactgcgcg
      601 cgtcaaactg aatgtcgata aagatttctc gctgaaggat tcttcgctgg aaatgcttta
      661 tccgatttat gatacgccaa caaatatgtt gttcactcag ggagcaatac atcgtaccga
      721 cgatcgtact cagtcaaata ttggttttgg ctggcgtcat ttttcaggaa atgactggat
      781 ggcgggggtg aacaccttta tcgaccatga tttatcccgt agtcataccc gcattggtgt
      841 tggtgcggaa tactggcgcg attatctgaa actgagcgcc aatggttata tccgggcttc
      901 tggctggaaa aaatcgccgg atgttgagga ttatcaggaa cgcccggcga atggctggga
      961 tattcgtgct gagggctatt tacccgcctg gccgcagctt ggcgcaagcc tgatgtatga
     1021 acagtattat ggcgatgaag tcgggctgtt tggtaaagat aagcgccaga aagacccgca
     1081 tgctatttct gccgaggtga cctatacgcc agtgcctctt ctgacactga gcgccgggca
     1141 taagcagggc aagagtggtg agaatgacac tcgctttggc ctggaagtta attatcggat
     1201 tggcgaacct ctggcgaaac aactcgatac agacagcatt cgcgagcgtc gggtactggc
     1261 aggcagccgc tatgacctgg ttgagcgtaa taacaacatc gttcttgagt accgcaaatc
     1321 tgaagtgatc cgtattgctc tgcctgaacg tattgaaggt aagggtggtc agacactttc
     1381 cctggggctt gtggtcagca aagcaactca cggactgaaa aatgtgcagt gggaagcgcc
     1441 gtcattactg gctgagggtg gcaaaattac cggtcagggt agtcagtggc aagtaacgct
     1501 cccggcttat cgtccaggca aagacaatta ttatgcgatt tctgcggttg cctacgataa
     1561 caaaggcaat gcctcaaaac gcgtgcagac agaggtggtc attaccggag caggtatgag
     1621 cgccgatcgc acggcgttaa cgcttgacgg tcagagccgt attcaaatgc ttgctaacgg
     1681 taatgagcaa agaccgctgg tgctgtctct gcgcgacgcc gaggggcagc cagtcacggg
     1741 catgaaagat cagatcaaga ctgaactagc cttcaaaccg gctggaaata ttgtgactcg
     1801 ttccctaaag gccactaaat cacaggcaaa gccaacactg ggtgagttca ccgaaactga
     1861 agcaggggtg tatcagtctg tctttactac cggaacgcag tcaggtgagg caacgattac
     1921 tgttagcgtt gatggcatga gcaaaaccgt cactgcagaa ctgcgggcca cgatgatgga
     1981 tgtggcaaac tccaccctga gcgctaacga gccgtcaggt gatgtggttg ctgatggtca
     2041 gcaagcctat acgttgacgt tgactgcggt ggactccgag ggtaatccgg tgacgggaga
     2101 agccagccgc ttgcgatttg ttccgcaaga cactaatggt gtaaccgttg gtgccatttc
     2161 ggaaataaaa ccaggcgttt acagcgccac ggtttcttca acccgtgccg gaaacgttgt
     2221 tgtgcgtgct ttcagcgagc agtatcagct gggcacatta caacaaacgc tgaagtttgt
     2281 tgccgggccg cttgatgcag cacattcgtc catcacactg aatcctgata aaccggtggt
     2341 tggcggtaca gttacggcaa tctggacggc aaaagatgct aatgacaacc ctgtaactgg
     2401 tctcaatccg gatgcaccgt cattatcggg cgcagctgct gtaggttcta cggcatcagg
     2461 ctggacggat aatggcgacg ggacctggac tgcgcagatt tctctcggca ctacggcggg
     2521 tgaattagag gttatgccga agctaaatgg acaggatgcg gcagcgaatg cggctaaagt
     2581 aaccgtggtg gctgatgcgt tatcttcagg ccagtcgaca gtgtctgtag ccgcagatcg
     2641 cgtaaaagca ggcgaaagca caaccgtgac gcttattgca aaagatgcac atggcaacgc
     2701 tatcagtggt ctttccctgt cggcaagcct gacgggtgct gcgtctgaag gggcgactgt
     2761 ttctggttgg accgaaaaag gtgatggttc ctatgtcgct acgctgacaa caggtggaaa
     2821 gacgggtgag cttctcgtca tgccgctatt caacggccag ccagcagcca ccgaagccgc
     2881 gcagttgact gtcattgcgg gggagatgtc atcagcgaac tctacgcttg ttgctgacaa
     2941 taaggctccg accgtcaaaa cgacgacgaa actcaccttc accgtgaagg atgcgtacgg
     3001 gaaccttgtc accgggctga agccagatgc accgcagttt agtggtgccg ccagcacggg
     3061 gacagagcga ccttcaacag gagactggac agaaacaagt aatggggtct acgtggcgac
     3121 cttaacgctg ggatctgccg cgggccagtt gtctgtgatg ccgcgagtga acggccaaaa
     3181 tgccgttgct cagccactgg tgctgaatgt tgctggtgac gcatctaagg ctgagattcg
     3241 tgatatgacg gtgaaggttg ataaccagct ggctaatgga caatcgacta accaggtaac
     3301 cctgaccgtt gtggacacct atggtaaccc gttgcaggga caaaatgtga cgctgactct
     3361 gccgaaaggt gtgaccagca agacggggaa tacggtaaca accgatgcgg caggtaaagc
     3421 cgacattgag ctgatgtcaa cggttgccgg ggaacacagc atcacggcct cagtgaataa
     3481 tgctcagaag acggtcacgg tgaaattcaa cgcggatgcc agcaccggtc aggcaaacct
     3541 gcaggtagac accgctgctc aaaaagtggc aaacggcaaa gatgccttta cgctgacggc
     3601 gaacgttgag gataaaaatg gtaaccctgt tccagggagc ctggtgacct ttaatctgcc
     3661 ccggggtgtc aagccgctta caggcgataa tgtctgggtg aaagccaacg atgaggggaa
     3721 agccgagttg caggtggttt cagtgactgc cggaacgtat gagatcacgg catcggcagg
     3781 gaatagccag ccttcgaata cgcagactat aacgtttgta gccgataagg ctaccgcaac
     3841 cgtctccggt attgaggtga tgggcaacta tgcgctggcg gacggcaaag ccaaacaaac
     3901 gtataaagtt acggtgactg atgccaataa caatttggtg aaagatagcg aagtgacgct
     3961 gactgccagc ccggcttcgt taaacctgga accgaatggc actgcgacaa cgaatgagca
     4021 agggcaggct attttcaccg ctaccactac tgttgcggcg acatacacac tcaaggcgca
     4081 agtgagtcag accaacggtc aggtatcaac gaaaactgcc gaatctaaat tcgttgcgga
     4141 tgataaaaac gcggtactca ccgcatcatc tgatatgcaa tctctggtgg cggatgggaa
     4201 atcgactgcg aagctggagg tgacactgat gtcggcaaac aaccccgttg gcgggaatat
     4261 gtgggtcgac attcagacgc cggaaggggt gacggaaaag gattatcagt tcctgccgtc
     4321 gaaaaatgac catttcgtga gcggaaaaat cacgcgtaaa tttagtacca gcaagcctgg
     4381 tgtctatacg ttcacattta acgccctgac ctatggcggg tacgaaatga agccagtgac
     4441 ggtgaccatt accgcggtgg atgccgatac ggcaaaggac gaggaggcga tgaaataatt
     4501 ttaagaatat gaaagtaaca ttctaataaa caataatggc gttgacatct tcaacgccat
     4561 tatttattga atttcctgac agtagtctcg ttataaattc atttagatat agcgaaaact
     4621 aaaccggacg aacggggata aaaatttcgc aaacaatgtt attgctatca atttcgacga
     4681 aattttcatt gtaataaaac cgctcaatat cactgccgtt cactctggct aaccccctgg
     4741 cgggtaattc catcagatag aggtttcgcg cccattcagg gtagtgctcc cactcacctt
     4801 catagcggaa agaggcatac agtcctcggt taattgtcca ctctttcggc atctctgaat
     4861 cggtttgttt gcataccgta ccggcaaaag tttcaaccgt gagcaaatcg accgattttg
     4921 ttgacggttc aaacgtggag acgaaataga tatccgtctt aaaattattc atacatctgg
     4981 ctaaatgatg acgtcgcagt ttgacttctt catccggtga tttattcgac acttcaatat
     5041 catatttaaa tgaattgcca aaaataggtg tctcaggaaa gttgactaat ctacactcgg
     5101 tcttttgctg ttgacgtatt aaaaaagaag ggaagatatt tgcgagatcc caataatcac
     5161 ggtggcggta tacacgggga gagcagccga ataacttttt aaattcgcgg ctgaatgact
     5221 gctgtgaatc aaaatgcaaa gagagtgcaa tatcaagcat agatttcgcg gtgagccgga
     5281 caagaatggc ggcacgacaa agccttcgtt tgcgaatgta ttctcccaaa ggcacatgca
     5341 tgaaatttcg gaacagaagc tggatgttgc gtctgctgta gccagatttc tgtgcgatat
     5401 cttcgattga aatagggtgc tcaagattgc actcaatcca ctccaggagc tgctgtagaa
     5461 tcttctgcct gatcatcaca ttctccattg attcttcgtc atatcgcaca ttttcgttat
     5521 gccctgggcc ctaagtgtat caaaaacagc aagggcgaag gcaaaagcac tggtagcgtc
     5581 tgcttcgttg tccccgaaaa tagagaggtt tcagtcctgc attattaatg aattttttgc
     5641 ataagtgata tcaaaatcca cgtactaatt tgaggttacg ttttaacgta gactcattgt
     5701 tcatgcctaa tggagggact gacagtggaa ttttccgtat taagtaacaa tctgaaaatg
     5761 ccgatgatgg gatttggtgt ttttcaggtt accgataaaa acgtgtgcaa acagtcagtg
     5821 ttgaacgcta tccgcacagg ttatcgactc atcgacactg cagcggtata cggtaatgaa
     5881 gatgccgtcg gtgaagccgt tcgtgaagct atatctgaag gtttatgtac ccgagatgag
     5941 ttatttataa cgtctaaatt gtgggtgcag gatatgttga atcaggatac ggcagcagca
     6001 ggtattgaag catcattaaa aaaatcggga ctagagtact tcgacctcta tttattgcac
     6061 caggctatgc gcgattattt cagtgcgtgg cgtgcactcg aagatgccta tgaagagggc
     6121 aaattaaaag caattggggt ttccaatttc tatcctcatg ttctggcgaa cttttgtgaa
     6181 acggtaagag ttaaaccgat ggtcaaccag gtcgagttgc atccctattt tgcccaacca
     6241 gaggcgctgg caaccatgaa gtattataac gtgcagcctg aagcatgggc tccgttaggt
     6301 ggtggacgac ataaaccctt tgaaaataat ctgcttcaga gtattgcaga tgcccatcaa
     6361 aaatcgattt ctcaagtcat tctgcgttgg aatattcaac ggggagtggt cgttattccg
     6421 aaatcgacac atcaacagcg tatcgaagaa aattttgcta tctgggattt ctcactgaca
     6481 gagaaagaaa tggcacaaat tagttcgctt gatttgggtt atgttgggga gtcggtaaaa
     6541 cattttaatc ctgaatttgt tcgtggttgt cttgctgtaa aaa
//
LOCUS       DAZFDP010000223         6483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFDP010000223 DAZFDP010000000
VERSION     DAZFDP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1036)
                     /locus_tag="ACHJ1G_004737"
     CDS             complement(<1..1036)
                     /locus_tag="ACHJ1G_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4766449.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     KAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETGKYTSVVDLARA
                     KANRQHTENPAEKIPPVTAAIHREYKQTWKTLDRELAYYLWPGDADAGNIDGSILRWA
                     KNEVIARDREDWKRISASMRKQPDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEG"
     gene            complement(1130..1321)
                     /locus_tag="ACHJ1G_004738"
     CDS             complement(1130..1321)
                     /locus_tag="ACHJ1G_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4766450.1"
                     /translation="MNTTFALVLTVYLVSGESLDLVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(1318..1506)
                     /gene="dicB"
                     /locus_tag="ACHJ1G_004739"
     CDS             complement(1318..1506)
                     /gene="dicB"
                     /locus_tag="ACHJ1G_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4766451.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            2074..2283
                     /locus_tag="ACHJ1G_004740"
     CDS             2074..2283
                     /locus_tag="ACHJ1G_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766452.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(2284..2922)
                     /locus_tag="ACHJ1G_004741"
     CDS             complement(2284..2922)
                     /locus_tag="ACHJ1G_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766453.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESMANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2934..3086)
                     /locus_tag="ACHJ1G_004742"
     CDS             complement(2934..3086)
                     /locus_tag="ACHJ1G_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4766454.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            complement(3352..3771)
                     /locus_tag="ACHJ1G_004743"
     CDS             complement(3352..3771)
                     /locus_tag="ACHJ1G_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766455.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            3871..4152
                     /locus_tag="ACHJ1G_004744"
     CDS             3871..4152
                     /locus_tag="ACHJ1G_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO4766456.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            4136..4561
                     /locus_tag="ACHJ1G_004745"
     CDS             4136..4561
                     /locus_tag="ACHJ1G_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4766457.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4768..5223)
                     /locus_tag="ACHJ1G_004746"
     CDS             complement(4768..5223)
                     /locus_tag="ACHJ1G_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766458.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            5302..6417
                     /locus_tag="ACHJ1G_004747"
     CDS             5302..6417
                     /locus_tag="ACHJ1G_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4766459.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1659 a         1433 c         1603 g         1788 t
ORIGIN      
        1 ttccttcatc ttcaaacacg ccctttgtag tcaggtattc agtaatgtat ttgttcagtg
       61 ccacagggtc tttgtgaatg tcgatcggac gttcacggac aaggccaaaa atagtctggc
      121 ggctgtagcg aagcgcatca ggttgtttgc gcattgatgc ggagatgcgc ttccagtctt
      181 cgcgatctct ggcgataact tcatttttag cccagcgaag gatgctgccg tcaatgtttc
      241 cggcatccgc atcaccaggc cagagatagt aggccagctc cctgtccagg gttttccatg
      301 tctgcttgta ttcgcgatga atggcggcag ttacaggagg gattttttct gctgggtttt
      361 cagtgtgctg tcggttggct ttggcgcggg caagatcaac aacagacgtg tattttccag
      421 tctctttgcg ctctgcgtcc tgccgttttt tccagttacg taattcagcc tgaatttcgg
      481 gccatttggt acccggctta catttgtgtt taacccatcc gatagcgaac agtttgcgtt
      541 ccggatacat agctttaatt tcaggcgttt tcatcagtgc ttcaacgata tgcccgtcaa
      601 aggtagccac gtcttcttgc agtaattcct gcgcgtcaat cgccatatca acggtgatgt
      661 tttcacatgt accgaactta accaggaccg cgttctgtac ttcaagggac agcttgtcaa
      721 aattgacgtt aataggatcg gattctggtt cgaccggaat aaaggaagcg gattcctcat
      781 cccagcggtt ttcctgcata tattcggtat cccaggagtc gatagcaggg cggggcatgc
      841 cgggtttatc ttcgcagaca agaaatttat aagcgcagtc ctgagcagca ggatattgct
      901 ccaggaattg ccaggtaaat ttggcacggg cgcggcgttc atcaccggct tcaatggcag
      961 tggctacagc aactgcgccc tcttctttta ttgcctgttc gtccggaatg gcggcgcaaa
     1021 taaagacttt actcattttg ttttaacctc attacagatt taagggtgaa caaatccctg
     1081 ccattgctgg catataaaaa tgaaaccgga tattaattac ggtgctgttt taaagtcctg
     1141 ccggtatttc gttattatta gtgtgagtag ttttatctac cggataacag ttaccgggaa
     1201 ttttttgttc tgctgctgca gccatgcatt ctttcattga accgtataag ccagtcacca
     1261 gatcaagaga ttcgccggaa acaagataaa ctgtcagaac gagtgcaaat gttgtattca
     1321 ttgtttatat cctttttgca gcaggtccag acgagccagc attgaaggaa tgcatacttc
     1381 atttaacagg tcctgctcga gttttctctg cttaatggcg tcttcaataa atgttttgtc
     1441 tccagtgata acgccaattt cgaaacgaag ttcagacgtg ctggcattac atgataactt
     1501 ttccattatc gcgtcctcaa caatgaattt tgtgatgcgg tgcctggtgc ctccaggtga
     1561 cgttaaccag ttaacaatta acgccggata cagagaatcc acccataaca ctgtttttgg
     1621 ttttaactgt tccgcgtgcg ctgagccgca ttcaccgcat cacaaaattc acttttaaaa
     1681 aagggcggca gagcagtcac ggagtaaaac tgataccgcc aaatgtcacc agaatattga
     1741 taacagaggg cgttgtagcg ggggtgtcac ttaagcgtat ggtcaacctg acaacccggt
     1801 gtcctcaact ggggaaggaa taaccccgcc atacttaccg ccgcgccatt tcgcggattg
     1861 ccacaaccgg aagcgcacgt tcgaagaaat ctaacgacaa gccttctaag ggaaagagct
     1921 tcgccgtacg ctttcgcgtt atgccctgac ttttcaggga aatatccttt cagtaaactg
     1981 tcagtaccgg attcttatcc gtgtccggcg cacgaccaca cgtgacagcg tgttggtctc
     2041 catttttaac ccagaacctc aatggaggat aaaatgccaa acaaaaaaag aaatccgctt
     2101 attgaaaaac agattgaatg cctggtaaat caactcaggc aatcagggtt attaaaaact
     2161 cattcagagt tgaggctcac agaatcagca ttcgacgata aattaaataa tgtcctttat
     2221 aatggcatta ttgattttaa tcgttctgtt ggtcgccgcg gccctgctgg tgtttcctta
     2281 taattaccag tcaatccaga gtggaccgtg ttcagcgtaa atataactgt acacatccag
     2341 attatatttg tggtctgtta agaacaggcc gcaaatacat gccgaagctt ccagtgcagc
     2401 ggctctgtta ctgaataacc atgtagcaac attccagcgt ttttctgcat cccagtcttt
     2461 ctcaaggcct gataccatga agaaaccgtt agtgttgcca tcaaataatt ctgtttccaa
     2521 atttttaagc aatgcctgat ggactcttgc caggtattcc gccggaattt cgccacgaat
     2581 tctaatgaga ttgtcataaa caaacatgtt ccccgcatat ggcgattttt ctttcttgtt
     2641 ttttaaacca gcatcatgag caaactgatc aatttcttct tccgttggtt tcgtattgat
     2701 gttttgcgct gtcgtttctg caattttatt tgccatactc tctgagtcgt gtttatttat
     2761 agacgcacag aaatacaatc cggtaaacgc atcgcgcaca ttacgagcca tattatcagt
     2821 gtcttttttc gttaccgatt ccaattcaag ttcgttcaga cgatgacgaa gtgtgtgtgc
     2881 tgcaatctcc tggattgaag gaggtaaatc tttaaattcc atcgtcaacc tcatcagtca
     2941 gtgtttctgg ctaaccagcg acgcgcgcca gcttcagttt taaacgtttt gcttttggta
     3001 tacgtcatcg cggtgaacgt accgtcctgg ttgggaaaca cgccgcatac cagagattcg
     3061 ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc cgggtagcgg
     3121 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc atacgcctcg
     3181 ggctggctac ttaccccctg accactgcct ggtaactcga agtattgccc tgcgttctgt
     3241 gggacggggt gggttggtat gaaaggaagg atacccatgg gtatttaaaa agtaaatacc
     3301 catgggtaat ttttttgatg tgtcttaact ggtgactggt tgtttggtga gttatgatgc
     3361 gttttgtgct ttctttttac ggatttcttc gtagatcata ttgtaatact gttttttctc
     3421 ttcaagagtt tttaataagt tatccgcttc actttctggc agttcgtcta agagatctaa
     3481 aaaaatacgt tgtcgtggcg ttagaaccct tgtttcataa ttggaggctg tgttcgttga
     3541 tgatgaaacg ataccatcca tccatccccg gggtaaccca aaggactctt ctataatctc
     3601 caccatatca tcagcgatcc gtttttttcc ttttttcccc tctgggtaca acattcttga
     3661 tacataagaa ggctcgcgac cgatctttct ggccacgtta accgctttac catcgcattt
     3721 ctcatcacga attttgatga gttgttgtcg tctaaattca tatttgtcca taggtaaata
     3781 atagatgcga ttaccgcaag gtaaacaacc caagggtatt gacttttgtt tacctgtagg
     3841 tattcttgct gtgtttacta aggagtagct atggaagaat taagaatatt tctcaattct
     3901 ctttcgtcag atgaacagcg tatgtttgca tgtgagtgtg gtactagcat cggttatcta
     3961 agaaaggcat tgagtaaagg tcaaatgtta ggggcatcgt tatgtgtcct tattgagcga
     4021 gccagtaatg gtgaagttac acgtcagcaa ctaaggcctt ttgattggat gaatatttgg
     4081 cccgagctgg aagataccaa aacgttaaca caaccactta ctaggagctt gattcatgaa
     4141 aatcaagcat gaacacatcc gcatggcgat gaatgcctgg gcgcgtcctg atggcgaaaa
     4201 agttccagca gctggaataa cccaggctta ttttgagttg ggtatgacgt ttcctgaact
     4261 gtatgacgac agccatccgg aagccctggc tcgcaatacc cagaaaattt tccgctgggt
     4321 ggagaaagac actcctgatg cggttaaaaa aattcaggcg ttgttaccag cgatcgaaaa
     4381 agcgatgccg cctccgctgg tggcccgaat gcgcagccac agttccgctt attttcggga
     4441 gttggtagag acgaaggaac ggctggtgaa agatattgat gatttcgttg catcagcgat
     4501 cgttctgttc gatcagatga atcgtggtgg cccggcagga aacactctgg ctgtgcatta
     4561 attgggtaat aaatatgagt aatgacaaaa aattgacact gagcgtttac gaaaacagtc
     4621 cgcacatctg gcgtggcggt ttatctgatg tggagctggc agagtggttg atacataaag
     4681 ctaatgcgct gctctggcgt ttgtcagcca gagaacagcg caaggaaacc agaataaagc
     4741 tggctgatgc agaagcgtgt gccgggctta ttgaggatta tacaaatctt ggtatttctt
     4801 cagcagagag tgatcccatt cagcctctga gcagggagtc aatccagcac gctggttgta
     4861 tggcacatct tgtaactgct cgtcaacatg aggtgggtat tggatcactt ccggtgggat
     4921 attcgctgat tccagagctg gttgaagcaa gaaaatcagt tcagaaaaag agagatgacg
     4981 cacttcaatt attgagagag cactatggcg cgataccaga atgcgaacag catcgatacc
     5041 ctgaaggtta tgaatggatg cagtctcttt ttgaagttcg ctaatcaata tgtcgagacg
     5101 aaggtatgtt tcggcgcgca gccaggctct gtaatccggg agcatttcgg ggctgttaca
     5161 ccagcggttt gttgctgcaa catttaatac atgagcctga taaaggcttt tcaaaaaata
     5221 catgtcgaac ctcctctggt tctgtcgatt gggaaccaca gattatatcc ggaggaaggt
     5281 tcggcaccag atgaggtagc catgcgtgat tacgcaaaag tttctccgcg attctggctg
     5341 ggagaaacgg ggagagaact tagaaaggcg ggtgcagaag cgcaagttgt tgctttttac
     5401 ctgatgacat cccctcacgc aaatatgctg ggtttgtatt acctgccagt tttatacctt
     5461 gctcatgaaa ccgggcttgg tctggaaggg gcttcaaagg ggcttaaaag ggctgttgaa
     5521 gctggttttt gtagctatga ccatgatgca gagatggtct gggtccatga aatggcagcc
     5581 tggcaggttg gggaaacgtt gaagcctggc gataaccgtt gtgcaggtgt caggaatgag
     5641 tatgcatcat tacctgaaaa cgcttttctg tcagtgtttt acgacagata taaaacggat
     5701 ttccatctgg atgtgaggcg gaataatagc cgaaattcgg taaggggctt cgaaggggct
     5761 tttaaggggc ttcgaagcca agaacaggaa caggagcagg agaaagaaca ggaacaggac
     5821 aaaaacacta tggttcatgg caaaaaaaac accacgaacc aggcagggga tgttcagacc
     5881 gtcaatcctg gtcagccagc aggcacgaca ccggaagccg attcagcgta tgcgctgaaa
     5941 gccgattcgg gcgctgtgca gcaggtgatg accgcagggt cggagcaatc acaccaactg
     6001 cagcagcctg aagccgattc cgccattcag cgggaagccg atcgggtagt cccggaaagc
     6061 accgggcagt ctgtgggacg agtggattat ccggatgtgt tcgaacaggt ctggcgggaa
     6121 tacccgttgc gtgctggggc aaacccgaag aaatccgctt tcagtgcctg gaaggccaga
     6181 ttgcgcgagg gggtgccacc agagaccatg ctggatggtg tgaggcgtta cgcgagatac
     6241 ctggcggcga ccgggaaagc gggaacggaa tttgttcagc gagcgacgac gttttttggg
     6301 ccggaccgga attttgaaaa cccctggttg ctcccggtaa gcggcacgaa caaccagcgt
     6361 tgtgtgaatc atatttctga accggatacc gaaattccgc cgggattcag ggggtgatgt
     6421 ggcatgaaaa acattgcggc agccggggtt cttgaacgta ttcgcagact tgcaccacag
     6481 gcg
//
LOCUS       DAZFDP010000224         6424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFDP010000224 DAZFDP010000000
VERSION     DAZFDP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..359
                     /gene="ydjO"
                     /locus_tag="ACHJ1G_004748"
                     /pseudo
     CDS             1..359
                     /gene="ydjO"
                     /locus_tag="ACHJ1G_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(363..1754)
                     /gene="tcyP"
                     /locus_tag="ACHJ1G_004749"
     CDS             complement(363..1754)
                     /gene="tcyP"
                     /locus_tag="ACHJ1G_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4766460.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1887..2477)
                     /gene="ydjM"
                     /locus_tag="ACHJ1G_004750"
     CDS             complement(1887..2477)
                     /gene="ydjM"
                     /locus_tag="ACHJ1G_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4766461.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2640..3308)
                     /gene="hxpB"
                     /locus_tag="ACHJ1G_004751"
     CDS             complement(2640..3308)
                     /gene="hxpB"
                     /locus_tag="ACHJ1G_004751"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4766462.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3455..3991
                     /gene="yniB"
                     /locus_tag="ACHJ1G_004752"
     CDS             3455..3991
                     /gene="yniB"
                     /locus_tag="ACHJ1G_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4766463.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4032..4892)
                     /gene="yniA"
                     /locus_tag="ACHJ1G_004753"
     CDS             complement(4032..4892)
                     /gene="yniA"
                     /locus_tag="ACHJ1G_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO4766464.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4998..5288)
                     /gene="ghoS"
                     /locus_tag="ACHJ1G_004754"
     CDS             complement(4998..5288)
                     /gene="ghoS"
                     /locus_tag="ACHJ1G_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4766465.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5389..6318)
                     /gene="pfkB"
                     /locus_tag="ACHJ1G_004755"
     CDS             complement(5389..6318)
                     /gene="pfkB"
                     /locus_tag="ACHJ1G_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4766466.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1662 a         1598 c         1573 g         1591 t
ORIGIN      
        1 tcctgccggg agcatttttg ctgcgattca aaaaaaatga gagtctccta aagtttcctt
       61 cgataatatc aatgcttaat atcaatcttc tttttaatcc actaaatctt ccgggaatgg
      121 gaagtggtgt gcttgaagat attatgagta ttccagatag ttcacttaga aaacgtctcg
      181 ggtatgaggt tttaagcttc tcactacagg cacattcatt gagccaggaa tgtattgata
      241 agttggacat ttttttcgct gacgatctgt ttaaatatga gtcagtatgc atcgccgcga
      301 tggaacattt aaaaagtaaa gcaacggccc ctatacaaaa cggaccgttg ccagcataag
      361 aattaacggt gtgccagttc ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc
      421 ttcagccact ggctggtcag cgtgccagct gtcatcgagc cactaacgtt tagcgccgta
      481 cggcccatgt cgataagcgg ttcaacggag attaacagcg ccaccagggt tactggcagg
      541 cccatcgcag gcagcacaat cagtgcggcg aaagttgcac caccaccgac accggcaacg
      601 cctgcggaac taacggtaac aataccgacc agcgtcgcaa tccacatcgg gtccagcggg
      661 ttaatgccaa ccgtaggcgc aaccatcacc gccagcattg ccggatacaa accggcgcag
      721 ccgttctgac caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaacg
      781 cccagacgac gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg
      841 gtaaaggcaa acgtcagcac aggccatacc ttacggaagt acttcagcgg actcacgcca
      901 ttaatgccca gcagaatgcc atgcactgca aacataatca gcagaccgag gtaggacgcg
      961 acaacgaaac ttcccagttt gatgatgtct tgcaggttag aacctgcaac cactttggtc
     1021 attagtgcca gaacgccgta aggggtcaac tgcatgacca ggcgaaccag tttcatcacc
     1081 cagctttgca gggtatcgat agcggttaag acgcgttcac ctttcggcgc atcatccttc
     1141 agcagtttca gcgcagctac accgaggaat gcggcaaaaa ttaccacgct gataattgac
     1201 gtcggattgg ctccggtcag atcggcaaac gggtttttcg ggataaagga caagaccagc
     1261 tgcggaacgc tcaggtcaga gactttacca acatagttac tttcgatggc gttcagacgt
     1321 gcagtttctg caccaccctg aaccagacct tcagccgtca aaccaaacag gttggtgacc
     1381 agcacaccga ccagcgccgc aatcagtgtg gtaaacaaaa gcgtaccgat ggtcagaaaa
     1441 ctgattttgc ctaactgaga tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg
     1501 aagactaacg gcataacgat catttgcagc agttgaacat agccgttacc aacgatgtta
     1561 aaccactgta cagaatcttt aagtacctgg ctgtcagaac cataaatggt atgcagggca
     1621 aggccaaaaa ccacacccat caccagaccc accagcactt ttttcgccag actccactgt
     1681 ttgtggcggg tctgagccag cgcaaacagc agtacaacga acaccacgat gttcgcaatt
     1741 aatggaaagt tcatccccgt tctcctgatc ttattatttc tatcactgcg tagcagtcat
     1801 agtgttggcg caagattaac agaactgtga gctggctctt atattcaaat ggaattgctt
     1861 attccagaaa ggtgattttt gcctttttag tattcaacct gatgcttaat aagccgatga
     1921 aactggatct gcaaggtatt gatcatttgc gatgaccaac gaaccgcgct gttttcgggt
     1981 aatgaatggg gcatccatac cgaccagaca aataatgcca tgcagataaa acgttccagt
     2041 tggttgccct tttggggaac caggataggc aagcggaaac gccagcgaca tggccagagc
     2101 aggggaacac cggcgggtgt cagcatatcg gcaagtatgt ggcttaaata acccagcacc
     2161 attccttgta gcgcatcagc cggaatgaac cagctttcag gaaccttaag gtaaaaggtt
     2221 gccagcagcg caaataccgc cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg
     2281 gcgatcggtt ttgatatcca ctttaatcgc tgcccaagaa acgactttgg gtgatcgatg
     2341 tccggtaaca aacacgtcag gattgcggaa gggacaatat gccaccagtc accctgtgcc
     2401 agcacgggcg tcagctcggc atttttggca aataccgcac aagcaataga aaagagaagg
     2461 tgaccttccg ccgtcatgat aaaactcact aaactgtcga tacgtacagt ataggatttt
     2521 tatacagtgc acggaagtgg tgacggtgta actctttctc ttaaattgac gattaatccg
     2581 tcagttgaat gcaaattcag ataaagaacc aggggcagcg agatcactgc ccctggtcat
     2641 taaccgagaa ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat
     2701 acaaaacgtg gatcattttg cgcttctggc gcaggaacga caatggaacg catgcgggct
     2761 gctttagagg cgatcatgcc atttactgaa tcttccagcg ctacgcaggt cagtgggtca
     2821 acgcccagtt ttgctgcgca gtcgagatat acttgcggat gcggcttgct gtaaggcagt
     2881 ttttcagccg aggcgagggc atcgaaactg tcgcgtaagt caaacatggt caacactttt
     2941 tccagcatat gtagtggtga cgcggaggcc agtcccacca ataaaccttg ttctttgcat
     3001 aacgcaacgg cttcgcgcac gcctggtaat aatggacgtg tctcttcaac cagtgaaatg
     3061 gcacgggcaa taacccgttc tactacttcc tgacggcttg gcccattcca cggttgccgg
     3121 gcgtaccaaa gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta
     3181 cgacgggaga tatccacccc caggcttgcc atcacatcca gttcggctcg atcccataaa
     3241 ggttctgagt cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg
     3301 gttgacatta caacctctcc tttagagcat tatgtgtaag gtattaagcc gtgcttatga
     3361 ttaagcgcct atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact
     3421 aagtgaaacg acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat
     3481 tgctgttttg aaaaggattt tgggttgggt gatttttata cctgcattga tctctacatt
     3541 gatttctttg ctgaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt
     3601 catgctcgac tttactcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa
     3661 tctgttctgg tataactccc cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg
     3721 ggtgattttc atcctgattt ttgtcggact ggcgttgcag gattccggtg cccggatgag
     3781 tcgccaggcg cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa
     3841 aggggaagag gggctaacgc gtgaacaaat tgagtcccgt attgttgttc cgcaccatac
     3901 tattttcctg cagtttttct cactatatat cctgccggtt atttgtattg ctgcaggtta
     3961 tgtgttcttt tctctgcttg ggtttattta atcaaaaggc cgcacggagc ggccattatc
     4021 ctcaacccat atcatgctgc taataatcta tccaatgact gctgagcaat aaccaaatgc
     4081 tgaccgccaa ataatcttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt
     4141 tcaaggaaat cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc
     4201 tgttcagtat gcagcggtaa catcgccagg tcgcactctc ggtcacccca gtagcaggca
     4261 gggtcgaaaa tgtacgggcc atccggaccc agcgcacagt tgccggacca taaatcgccg
     4321 tgcaacagag aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctcgacgatg
     4381 gcgtcgatat tgccgaaagc gatccctttc tctgctgcca gttccaactg ccagccaatc
     4441 cgttgttcag caaaaaacgt tgaccagcga cgttgccagg tgttgggctg tggagttgtg
     4501 gagagcgagt tatcaaaatc gaggccaaat tgtggttggt cactccattg atgtaaacgc
     4561 gcaatttgct gaccaagaat aaatgcgcta tgcgcatcca gcggacgagg tgggagataa
     4621 tccatcacca gaaaactgta gtcacggtca gcgcctactg cccaaacctt aggcacggtg
     4681 acggttttac tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc
     4741 agttcccttt catcacattt gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg
     4801 gcatggactt ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc
     4861 tcgctcaaaa gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat
     4921 cataaggtta gcgcaagaat gcggtgtaaa aacacgagct aacgcacacc acagactgat
     4981 tattgcccaa aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggtg
     5041 atttcaggat ctttgccggt tgcactttgg gtaagccccg aaaccagttc tctaatttct
     5101 tcttcacttt gggtactgat aagtccaaaa gtgttagtcc ccagctcatg gatatttccc
     5161 tcatcatccg tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta
     5221 atttcagtca atgtatcctc atgcaacatg acggttatga cataacggac aagatcgcca
     5281 ctggccatag ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt
     5341 tgccgacagg ttggtggtga ttcccccaat gctgggggaa tgtttttgtt agcgggaaag
     5401 gtaagcgtaa attttttgcg tatcgtcatg ggagcacaga cgtgttccct gattgagtgt
     5461 ggctgcactc cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc
     5521 cagtttcagt gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtac tctggctttt
     5581 caccggtggt ggcaccacct gaatgcagtt ttcactatca acacccagcg ctccttgtgg
     5641 accaagggaa acgacaaccc gtttggcctt gccgctatta acgatttcct gcgcggcttt
     5701 gcggacatcg tctggctggg tgagttcgcg attcaccagc gcactgagtt ctttttggtt
     5761 aggcttaacc aactcgatgt taccaatcgc cagtgctgca cttaacgctt cgccagaact
     5821 gtcgatgatg cagcggatcc cttggttttg cgcagcggaa atcagttggg ttaatttttc
     5881 cagcttcaca cctggcggca ggcttccgct tatgaccagg atggccccgg attcaatttc
     5941 cagaacttgc tcttcaagct ggcgaaactc atcttcattt aatgccgcgc ctggcataac
     6001 aaaacgatac tgctcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc
     6061 tttggcttct acagtagcga cggggacatt ttcatccgcc aacagagaaa ccaggtgttc
     6121 gccggtcgcg ccacccgccg ggaagatcgc tgtggcactg cctccaagat gggcaatggc
     6181 gcgggcgacg ttgatgccgc cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt
     6241 tccttcggga taaatttgcg gggtaattgt tgcgctatcg agagagggcg caagtgtcaa
     6301 cgtatagata cgtaccatca tttcctccta taggctgatt tcagtctggc accgaatcag
     6361 ccgaaagtga agaaattaac aatatgattt attagagctt tatttaaaat tttgcagata
     6421 aata
//
LOCUS       DAZFDP010000225         6308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFDP010000225 DAZFDP010000000
VERSION     DAZFDP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(215..1885)
                     /gene="betA"
                     /locus_tag="ACHJ1G_004756"
     CDS             complement(215..1885)
                     /gene="betA"
                     /locus_tag="ACHJ1G_004756"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4766467.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1899..3371)
                     /gene="betB"
                     /locus_tag="ACHJ1G_004757"
     CDS             complement(1899..3371)
                     /gene="betB"
                     /locus_tag="ACHJ1G_004757"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4766468.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3385..3972)
                     /gene="betI"
                     /locus_tag="ACHJ1G_004758"
     CDS             complement(3385..3972)
                     /gene="betI"
                     /locus_tag="ACHJ1G_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4766469.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4101..6134
                     /gene="betT"
                     /locus_tag="ACHJ1G_004759"
     CDS             4101..6134
                     /gene="betT"
                     /locus_tag="ACHJ1G_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO4766470.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1294 a         1731 c         1737 g         1546 t
ORIGIN      
        1 ataatatatc cagaaaataa aatctgtcgc agataaggtt gtattaatag tctgtatcag
       61 gaatgttcgg gttaaatatc agcaaaaagc cagcatcatg gatactggat atgaagcatg
      121 agagttacct cggtgtttat ataaggattc ggtccccctc tctggaacgg taactctcaa
      181 tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc accggcatcc
      241 catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca cgaatcatat
      301 ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg ataatctgcg
      361 gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg ccttcgccgt
      421 caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg aaggcggttt
      481 cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc gtgccggggc
      541 tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg cgggtgatgc
      601 gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaatcg
      661 ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca
      721 ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg ttatagttaa
      781 tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca cggctgcgaa
      841 taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca aacagccact
      901 ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa accggttctt
      961 tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg acgccgggta
     1021 attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg acgccggagc
     1081 gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct ttgttggccg
     1141 ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc gcgcgtttgc
     1201 cgtcaaaaat gatgtgatcg gtcatagcgt gaatacgaat ggtcaggtta gggcgcgatt
     1261 tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc gtgacggtgc
     1321 gatccatcgg accaaaacct tcctgctgat aaccgttgag atcgtccgtg cgcgggtagc
     1381 ccgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg
     1441 tagtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca tcgcgagtct
     1501 cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga ccgggttctt
     1561 gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg ttgatcagcg
     1621 acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc ataaacggtt
     1681 caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac gccagcgctg
     1741 cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca agcagcagca
     1801 cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac
     1861 caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta
     1921 gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc
     1981 tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc
     2041 caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc
     2101 aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc gcggcgaatc
     2161 acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat ctcttcgcgc
     2221 acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca tgcgccgtta
     2281 tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg
     2341 cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggctgaag
     2401 ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaattttc
     2461 tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccgtt ggtacacacc
     2521 tgaccggagc tgaagaagtt cgccatcatg gcgatatcgg cggcgagatc gagatccgca
     2581 tcgtcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc tttcagggaa
     2641 gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact
     2701 ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgccccc ccccggcaac
     2761 acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag ctttaacgcg
     2821 gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc cagcgccggg
     2881 gcggatttcc acagggcaat ctggatcggg tagttccatg cgccaatccc cgccaccaca
     2941 cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat ctggctgcct
     3001 tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt aacgatatcg
     3061 acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt tgcgagttcg
     3121 tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg
     3181 gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg atcgacatcc
     3241 tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg
     3301 aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc
     3361 attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg
     3421 ggtcagcgag tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca
     3481 taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa
     3541 ctcgcggcga aattcgctca ccagattcga caataagcgg cggctactga cctgctgtaa
     3601 acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg ctttcatcgc
     3661 cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta atcgctgctc
     3721 tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt
     3781 gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat
     3841 ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac
     3901 ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg actgcatccc
     3961 caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt
     4021 gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt aaatggtaac
     4081 aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca
     4141 atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg acaacgatcc
     4201 tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct
     4261 tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt
     4321 gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc
     4381 tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg
     4441 tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg
     4501 ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt
     4561 atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca
     4621 tccgctcggc gctgtacccg atcttcggta aacggattaa cgggccgata gggcactcag
     4681 tggatattgc agcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg
     4741 tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcaa
     4801 aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg
     4861 ataaggggat tcgtgtgtta tcggaactta acgttgcgct ggcgctggga ttgatcctgt
     4921 tcgtattgtt tatgggcgac acctcgttcc tgcttaatgc gctggtgctg aatgttggcg
     4981 actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac cgtccagttg
     5041 agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg
     5101 tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca
     5161 cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc aatagcgcgc
     5221 tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc
     5281 gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca
     5341 ccattactgg cctgctgttt tatgtgacct cggcggactc cggtgcgctg gtgctgggga
     5401 atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg cgcgtcttct
     5461 ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg atatccgcgc
     5521 tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc ttcgtgatgg
     5581 cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata
     5641 ccgcaccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc
     5701 tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg
     5761 caatggaaga agtggcgcag gagctgcggt tgcgcggcgc atacgtggag ctaaaaagcc
     5821 tgccgccgga agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag
     5881 aacaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc tttacctacc
     5941 gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc
     6001 agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt
     6061 acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt
     6121 tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg gcgcgggaat
     6181 cattgactcg ctgtaaaaaa ttatgcatat attatatatg attgtcatgt catatcctgc
     6241 aattcctctt gtatcacttc atgattttat attttattaa ttaacgattt tattttgtat
     6301 tagctgaa
//
LOCUS       DAZFDP010000226         6290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFDP010000226 DAZFDP010000000
VERSION     DAZFDP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..1500)
                     /gene="gndA"
                     /locus_tag="ACHJ1G_004760"
     CDS             complement(94..1500)
                     /gene="gndA"
                     /locus_tag="ACHJ1G_004760"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4766471.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1611..2018)
                     /locus_tag="ACHJ1G_004761"
     CDS             complement(1611..2018)
                     /locus_tag="ACHJ1G_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO4766472.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(2009..3217)
                     /locus_tag="ACHJ1G_004762"
     CDS             complement(2009..3217)
                     /locus_tag="ACHJ1G_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4766473.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(3217..4347)
                     /gene="wecB"
                     /locus_tag="ACHJ1G_004763"
     CDS             complement(3217..4347)
                     /gene="wecB"
                     /locus_tag="ACHJ1G_004763"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4766474.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(4344..5450)
                     /locus_tag="ACHJ1G_004764"
     CDS             complement(4344..5450)
                     /locus_tag="ACHJ1G_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO4766475.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(5452..>6290)
                     /locus_tag="ACHJ1G_004765"
     CDS             complement(5452..>6290)
                     /locus_tag="ACHJ1G_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFO4766476.1"
                     /translation="VLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVLEA
                     AIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGNVM
                     ASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKA
                     PAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRVPP
                     DLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDYEL
                     DS"
BASE COUNT         1850 a         1435 c         1220 g         1785 t
ORIGIN      
        1 ttaagcagtg aaatttagtc tataagataa ttggcaaaaa aaaagcccgg cgtaatatac
       61 gccgggcctc aatttattgt tggttaaatc agattaatcc agccattcag tatggaacac
      121 accttcttta tcaatgcgct tataagtatg cgcaccaaaa tagtcacgct gagcctggat
      181 caggttcgca ggcagaacag cggcacggta gctgtcataa taggcaaccg cagcggcgaa
      241 ggtcggaacc gggataccgt tctgtactgc ataagcaacg acatcacgca gcgcctgctg
      301 gtagtcatcg gcaatttgct tgaagtacgg agccaacagc aggttagcga tctgaggatt
      361 ttcggcataa gcatcagtta ttttctgcag gaactgcgca cggatgatgc agccagcacg
      421 gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tactcttcag acgctgcgcg
      481 cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg cacggcgaac
      541 tttttcgatg aactcagcct tgtcgccagc tggctgcgct tgcgggccag agagaacttt
      601 agatgcggca acacgctgat ctttcagaga agagatataa cgtgcaaaca cagactcggt
      661 aatcagcgac agcggttcac cgagatccag cgcgctctgg ctggtccatt taccggtacc
      721 tttgttagcc gcttcatcca ggatcacatc aaccaggtag ttaccgtctt catctttttt
      781 ggtgaagatg tctttggtga tgtcgatcag gtagctactc agttcaccgt tattccactc
      841 ggtaaaggtc tgtgccagtt cttcgttaga gagattcagg ccgcctttca ggagagaata
      901 ggcttcagca atcagttgca tatcaccgta ttcaataccg ttgtgaacca tcttcacata
      961 gtgacccgcg ccatcagcac caatataggt aacgcacggt tcgccatctt cagcaacggc
     1021 ggcgatttta gtcaggatcg gtgcaaccag ttcataggct tctttctgcc caccaggcat
     1081 aatggaagga cctttcagtg cgccttcttc accaccggag acaccggtac caatgaagtt
     1141 aaaaccttct gcagaaagct cacgattacg acgaatggtg tcctggaaga aggtattacc
     1201 accatcaatg atgatgtcac ctttatcgag gtatggctta agggaatcaa tagcagcatc
     1261 cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca gagattcaac
     1321 aaactctttc accgtatagt aaggaaccag tttcttgcct ggattttcgg caatcacttc
     1381 ttccgtcttt tcacgggaac ggttgaaaat agagacggta taaccacggc tttcgatgtt
     1441 gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt gctttgacat
     1501 tgtttactcc tgtcaggggt ggtcacgcgg ggttacgcgg cttgaagaag ctaagatggt
     1561 aacccagcat gtatagaata agatagaaga ataaacggtg gtgtatcgcc ctataaccac
     1621 ttagaaaaag ccttagcttt cagtgggtca aatggcccct cctttatctc taacataagg
     1681 gcttttggtg acaggcattt gacactatgt atatctcctg gggaaaattc cacgacactt
     1741 attcccgtac cgtctccaac aacaaacttt ttttggagct caccattttg ctcatacaaa
     1801 caaacttcta attgcccttg catgacgaca aacatttccc actgatgcgg taactcatgc
     1861 caatggggtt caacatagct gtcgcgtaca aatgcaataa gtaaacgttg aaccttctcc
     1921 tgatgagatg cgtgcaataa ataatgagcg cgcaaacgtt cagaatttgc tgcagtgcga
     1981 aaaagttcat ccataatatt gtcatcaact aaacgcattc tcctgcctcc agtcggactt
     2041 cgatagtatg tgccgccgat tcaaccgaaa attgagactt taacaacaca ttggcgttct
     2101 gacctaactg ttttcttaaa actgaatcac taagaagcaa ttgtgcagat tcaaacagtt
     2161 tatcatcttc accattaaca tgaatgaacc cagccctgtg cttattaatt acatccatta
     2221 gatcattacc gccattcaca ctcccaagga tcgggattga ttgaaccata taccctagta
     2281 attttccggg gaaattatgt gaagaatggc gagacgaaag ggagaacagg ccaacatcaa
     2341 cttcagataa aattaattta aactcttcct ggttcactga aggtagatga gtgaaattag
     2401 ttaaattcca ttctgcagaa agagatttaa tcaggtcaac ttcatcaccc tgccctataa
     2461 acaggaaatg agcatcatga tgacgcatca tattacgcgc aaggcgcatt aagtttgcca
     2521 tatcctgagc atgcccaata ttaccgccat agaaaaaaat gattttatct tttagagcgt
     2581 atttttgacg aagtgaatga taatcatcgc cggcagacac aggagtcatt gaggcccaat
     2641 tacgtaaaac ttcacacgga taacctttat tggcctgacg aaatatctca agattcttat
     2701 cagacattaa cccaatccag tcagcctgtt gatatgattt tttttcaaaa tacctgaaat
     2761 atttttcaat tggcgagccg gcttttatca taccagcatc aatgacccac tgtgggaaca
     2821 tatcccttag gaccagatag cttgggcact ggcatcgctc tttaattttt ttaaccaagc
     2881 ctccccaaaa aatagagggg gaataataaa cgataccatc aaatgtatca tgttggatga
     2941 gatgctttaa tgcgtgccat gcacgaaaag ataaaagagt ttcatttatg gcacgtttag
     3001 ccttacctat atcctttaaa ggtccacttt tgaaacgcca aacctttata ccatcagtca
     3061 tgctaacaga atagattgcc tgtaatgtgt tgtcaggcgt aattacagtt acatcatggc
     3121 ctctgctcag caattccagg cctaactcat gaaacatttt agccccaact cgtgtgctat
     3181 ggggcaaata atcatcaatg attaatgcaa gtttcattag tattgcttcc agacaacccg
     3241 tttaacgtag tcagtatatg aatggataat acgcacaact ttatctgaaa catttggcat
     3301 actatagtca ctgacctgac gaagtaagcg tacttctcca cgaggctgtg ttgcaataat
     3361 ttctaatgcc tgtaacacac gggcagactc aagaccaacc atcattacag ccccttcttc
     3421 gaagccttca gggcgttcgt gcgcttctcg tatattgagc gcagggaagt tcataatgga
     3481 ggactcctct gtaatagtcc cgctatccga taaaacagca cgtgcatttt tttgcagatg
     3541 gttgtaatcg tgaaatccta atggcttaag tagcaagata tttttatgaa attgaatacc
     3601 gttttcgttg atgcggttac gagtgcgcgg atgagtagaa acaactaccg ggatatcata
     3661 tttttcagcc acggtattaa gtatatttgc cagtttcgca agctgtttag gggtatcaac
     3721 attttcttct ctgtgggcac ttaccacaaa gaaattccca ggtgttaaat tcaggcgaga
     3781 aagtacatcg gaaccatcaa tctgcgacat ataatgagtg agtacttcaa acattgggct
     3841 accggtttta ataatcctat cggctggtac accttcagcc aaaagatatt cacgcgcgat
     3901 atcactatat gtcatattga tatcagcggt gtggtcaact atttttctgt tagtttcttc
     3961 cggtacgcgt tgatcaaaac aacgattccc agcctccata tggaaaatcg gaattcttcg
     4021 acgctttgct ggtattgctg aaatacagga gttagtatcg ccaagaacta acatggcttc
     4081 tggtttttcc tgttcaaaga cctcatcaac tttaatgata acttgtccaa tagtctctgc
     4141 tgcattttta cctgcggcat taagaaaata atcaggtttg cgaacaccca aatccttgaa
     4201 aaaaacctca ttcaattcat aatcgtaatt ttgcccagta tgaacaataa ggtgatcaca
     4261 atattcatct aattttgcaa ggacacgcga gagtcgaata atttctggac gagtcccaac
     4321 aaccgacatg actttcaatt ttttcacgat aaaactctcg ctatagtatc tggttcagaa
     4381 cgattaaata tttcatttgc ccaaagcata acaactagct catccgagcc attattagta
     4441 atgtcatgcg tccatcccgg aactgtttca acaattttaa aatcatctga tgaaacatta
     4501 agttcatatc gttcacccgt gacaatattt tcaaatttga aacaagcact tcctcggatg
     4561 ataataaatt tctcattttt ggaatgatga taatgccccc cccgagtaat tcctggatgc
     4621 gcagtaaaaa acgaaaactg gcccgcgttt ttcgttttca atacttcaca gaatacccct
     4681 ctgtcatcac tataagaagg aaccgtatac gcaaactgtt taggagaaag gtaacttaac
     4741 catgttgagt acaatgctcg agtgaatcca ttacctacat cttcgatgat taatgtttgg
     4801 cgactttctt taaatcgata aattaattgt gccacctcac caacagtaac agaataaatt
     4861 ggaccaaatg tcctgtaacc agtttcgttc gctccttcta atagctttaa tatgtcagaa
     4921 caaaagtcat ctatatacac caaatttaca actgctgaag gatcattaat tgtaatagct
     4981 tcatcatttg caatgcgatg gcagaaagtt gctataaagg agttataatt tggttgacac
     5041 cacttaccga atacattcgg caagcgataa atataatatt tagcgtttgt cgtttcccca
     5101 tactgctgaa tgattttttc cgcaactgct ttactctttc cataagcgtt atcacattca
     5161 gcttggatgg atgaactaag catgatagga gtatctttat tatttttttt cagaatatca
     5221 acaatctgtt gagttagatc gcaattcccc tcttcaaact cactctcctt cacaggacga
     5281 tttactccag caagatgaaa aatgaaatct gcctgcttaa gtccctgctc taaatccgtc
     5341 aaagaagagt tacgatctat cgcaataagt tcgttatatc cagcttcctt aaggcggaaa
     5401 accaaatttc gaccgataaa ccctgcagcg ccagtaacta aaattttcat attatgaatc
     5461 caactcataa tcttcaccag aacgaagtgc ccggataaaa ggaagtttta gcagtaattc
     5521 tttcattccc tcaacatcta atctctcagt attatgagaa ttataatctt ccacttccga
     5581 gatacgacgg tcaccatgtt ccacatattt tccatagttc aaatcgcgga gatctggtgg
     5641 aacacgataa taatcaccca tatcttccgc tgcaatcatt tcctctcggc tcaataacgc
     5701 ttcgtaaagt ttttccccgt gtcgagttcc aataatattg attggatgct catgggcatt
     5761 tagcaattcc ttaagtgcaa tggctaatgt ttgaattgtt gccgcaggtg ctttctgaac
     5821 gaaaatgtca ccattatttc catgttcgaa agcataaagg accagatcga cagcatcctc
     5881 aagcgtcatc atgaaacgag tcatattggg atcggttatg gtcaatggtt taccagcttt
     5941 gattagatca acaaataatg ggatgaccga tccacgtgaa gccattacat ttccataacg
     6001 agttccacag ataactgttt ttgaactgtc aagattacgt gattttgcaa caataacttt
     6061 ttccatcatt gccttagata tgcccattgc attgatagga taaacggctt tatcggtact
     6121 caagcacacc acgcgtttaa tcccattcgc aatagcagcc tccagaacat tttccgtacc
     6181 cagaacattt gtcttaaccg cctccatagg gtgaaattca catgaaggaa cttgtttaag
     6241 ggctgctgca tgatacagaa aatcggcacc acgcgttgca tttagaacgg
//
LOCUS       DAZFDP010000227         6204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFDP010000227 DAZFDP010000000
VERSION     DAZFDP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..763
                     /locus_tag="ACHJ1G_004766"
     CDS             56..763
                     /locus_tag="ACHJ1G_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4766477.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            760..2187
                     /locus_tag="ACHJ1G_004767"
     CDS             760..2187
                     /locus_tag="ACHJ1G_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4766478.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2197..2751)
                     /locus_tag="ACHJ1G_004768"
     CDS             complement(2197..2751)
                     /locus_tag="ACHJ1G_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4766479.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2852..3559
                     /locus_tag="ACHJ1G_004769"
     CDS             2852..3559
                     /locus_tag="ACHJ1G_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4766480.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3556..5007
                     /gene="djlC"
                     /locus_tag="ACHJ1G_004770"
     CDS             3556..5007
                     /gene="djlC"
                     /locus_tag="ACHJ1G_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO4766481.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(5067..>6204)
                     /gene="hscC"
                     /locus_tag="ACHJ1G_004771"
     CDS             complement(5067..>6204)
                     /gene="hscC"
                     /locus_tag="ACHJ1G_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4766482.1"
                     /translation="DVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRADVAR
                     TTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLLNRLR
                     VPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIVALGA
                     AIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRVETYS
                     TMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGLLEVD
                     VLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEELWAR
                     ALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
BASE COUNT         1591 a         1417 c         1524 g         1672 t
ORIGIN      
        1 acgagcctcg caatgcggct aatattcatt taatgaatat ttaaggataa attatatgga
       61 catggagtcg caaaaaatat tgtttgcact ttccactccg atggaaatac gcaatgaatg
      121 ctgtttacct tcgcattcat cacccaaaat gtatttaggt acatgctttt ttgacctttc
      181 ttcctcctgg ggaattgatg cccgtgatga cctgctgcgc acaattcacc gaatgatcga
      241 taacggtcat gccgctcggt tggcagggtt ttatcaccgc tggtttcgtt attcgccatg
      301 tgaatggcgt gactatcttg ctgaactaaa tgagcaaggt caggcttatg cgcaatttgt
      361 cgccagcacc gccgaatgct gtggtgaggg aggaattaaa gcctgggatt atgtccggat
      421 gggtttttta agccgaatgg gcgtacttaa taactggttg agcgaagaag aaagtctatg
      481 gatccagtcg cgcattcatc tacgggctct acgttattat cgtaactggc ggcaatactt
      541 tgccggttat acctttggtc ggcaatattg gcagtctccc gaagatgatc atctgcagtt
      601 attacgcgaa ttcttagcgc gtaaagaata cgacgattcc ggcaatgata tgttttatca
      661 attatttgcc agtgatgatg cgtattaccc taccttgtcc tggcaaccat tggcttacta
      721 ttctgcatgc ccggaaacgc ttaaggatat gagcgaccta tgaaaaattg ctggaagatt
      781 ctcgaaatag aggaaacgac tgacgtcgat attatccgcc gcgcttatct ggcgctgtta
      841 ccgtcctttc atccagaaac cgatccgcag ggttttaaac aacttcgtca ggcgtatgag
      901 gaggcgctac ggattgcgca gtcgcctgct aaatctgttt ggcaaccaga agaatatgag
      961 gtagcagaac atgaaattct gctcgccttt cgtgcgttac ttgcctctga tagcgaacgt
     1021 tttctgccct ccgcctggca gcgattcatt cagcaattaa attattgctc gatggaagag
     1081 attgatgaat tacgctggtc gctgtgcaca atcgccatga acactgccca tttatccttc
     1141 gagtgcgtgg tgttattagc agaaagattg cggtggttgc aagaggaaaa cgtcggggaa
     1201 atagacgaag aagaactgga atccttttta tatgccattg cgaaggggaa tgtttttaac
     1261 ttccagacca ttctgcatct gcccgttgcc gtgcaaaatg acactatcga tttttaccaa
     1321 atgttcgctc ggatttggtc atcgcatcca gaatggctga cattgtattt agcgcaacat
     1381 cgcgcagtga ttatccccga tgatgcaaaa ctgcacagaa atttactccg ctggtatagc
     1441 gcaggtcgcc tgggaattcc cgaactcctg gattacgccc ggtcgtggcg ggaagctgaa
     1501 tctgataatg aagatgcgcg ttattatgaa tacgcacaac gcgtctattg tggagaaggc
     1561 gaaagcttgc tggcagaact ttgtgactac tggcgcgagt atccctccac ccaggcggat
     1621 gctttaatat tgcaatggtg ccgtcagcat cgggtcgatt attacccgtt agtggtgatg
     1681 atgattgaag ctcgtgaact ggttaacgat cagggaaaac cgctacttta tatccccggc
     1741 gacagcgccc gtacgcgttt tcatttatac gaaatactca gcgatgaaaa actctctgct
     1801 ctggggcgtt cactggtcga gatggttttg cacaaaggac gtaagccgcg gatctcactc
     1861 acgcgtgata cagaacatcc cttatggcca ttatatttag ttgccaaaca attagtgcag
     1921 gcgaaccaac caaccgaaga atcattaatg ccgattgtga gccgccttga tgcagaagat
     1981 cgttgtccac tggaagcatt aattattcgt cgattattaa ttcaggcggc gaattttacc
     2041 gagaagcaaa ctgttgaacc ggagccgcaa ccgcagccaa tgcccgttga cgatggtggg
     2101 ccaggctgtc tgggcatcat taaaattatt ttctatattt ttatctttgc tggtttgata
     2161 gggaaaatac tccatctgtt cggttgacag tcgacgttaa tgtgcgggaa gcccggcaag
     2221 taatctttcc gcatttttgt aatgttgttt ggcggccagt tttaaccatt tttgagattc
     2281 ataatggtta acattaatac ctgagccatt tctgtagatc aaacctaaag tatattgagc
     2341 atgagcatac ccttgttcag ctgcttcctt ataccagaga attgctttac gggtgtcggg
     2401 tttgaccccc agacctttga ggtagatcca ccccagtttg ctttgtgcgc gcggattttc
     2461 ctgttctgct gattgctcat accagtatct cgcctgtgta taatccagtg gtttaccgtc
     2521 gctaccatga agataaaatt ctccaaggcg atattgcgac gttgagtgac catgaatcgc
     2581 ggaaagctcc gtggccgccg tggtaaacgg tagactgccc ggtgagctta aataatcaaa
     2641 ggcataattc accatggctt tatcacgcaa cgttgttttc ttggggtggc taatacaggt
     2701 aattgctgca actataaata ttgcgaatat atacattaat tttttattca tatgtgaatg
     2761 cttcgatttt ctttttattt tgaaagcgtc aagtttatac tcatcaataa taaaaagtag
     2821 tcagttaatg cacttaaggg attattaaca aatgaataag gaagaacaat atcttctttt
     2881 tgcgctttct gcgcccatgg aaatacttaa tcagggttgc aaacccgcgc atgactcgcc
     2941 aaaaatgtat accgggataa aagaattcga tctctcatca tcctggggaa taaataatcg
     3001 tgatgattta attcagacta tttatcagat gactgatgat ggtcatgcca atgatttagc
     3061 tggcttatat ctgacatggc atcgctcttc ccctgaagag tggaaagcat taattgctgg
     3121 tggctcggag agagggttga tttataccca attcgttgcc caaaccgcta tgtgctgtgg
     3181 agaaggggga atcaaagcct gggattatgt tcgaatgggg tttctgtcac gggtcggtgt
     3241 gctcaacaac tggctgacgg aagaagagag tttatggctg caatcgtgcg tctatgtacg
     3301 agctcaccat tactatcaca gctggatgca ctatttttcc gcctattcgt taggacgtct
     3361 ctactggcaa tcttctcagt gcgaagacaa cacttcgcta cgcgaggcgc taaccctgta
     3421 caaatacgat agcgccggga gtcggatgtt cgaagagctg gcagcgggaa gcgatcgttt
     3481 ctatgccacg cttccctggc agccattaac tgtccagcct gaatgcccgg taacgcttaa
     3541 ggatgtgagc gacctatgaa aacatgttgg caaatcctcg aaattgaaag cacgacgcaa
     3601 atagacatta tccgccaggc ttatcttgct cgcttaccgt tatgtcatcc cgaaaccgat
     3661 ccgcaggggt ttaaagcatt acgccaggcc tatgaagagg cgctgcgact ggcggtaaat
     3721 cctgtcgagg aagcagatga tgaagaaaaa gatgccgccg ctgaacatga aatactgcgt
     3781 gcattcagga cattactgga ttcagaaagt gatcgttttc agccctccgc ctggcagaaa
     3841 tttattcagc aattaaatac ctggaacatg gaggatgtcg atcaattacg ctggccgctg
     3901 tgtgcaatcg ctatagaagc gcgatatctt tcattaaatt gtgcttcttt gctggcagag
     3961 cgtttgaact ggcattcgtt taatgacagc gaaggaatgg atgaggaaga aagggaggct
     4021 tttcttgagg ccattcaggc tggtgattgt ttcgatttcc ttagccttct ggaatatccc
     4081 gttgcgttgc agaaccagac tgttgagtat tacttcgcgc tggaacgttg ctgccgttac
     4141 catcctgact acgtcactgc gtttttggcg atggaaggtc cgtggttcat tcctgatgat
     4201 gcaaagttac atcgcaaact gttgcgctgg tacagctcgg tgcaaacagg tatggcggaa
     4261 ctcattcctg tagccaaaca gtggcaaatg gaagaaccag aaagcgaaga tgcccgatat
     4321 tacttgtgtg ctcaaaggtt gtactgcggc gagggggaaa gcctgcttgc cgatctctgc
     4381 gcgtaccggg aaagttaccc atctacacaa gctgataatt tgttgttgca gtggagcaag
     4441 cgtcattgcc cggattattt cgcgttatta gtgatggtta tcgaagcgcg gagcatggta
     4501 gatgcgcaag gtcaaccgct gaaatatgtt cctggtgaga gcgcccggac gcggctgtta
     4561 tgggcggaaa ttttacatag cggaaaatta tcgccgttag gtcaatcgtt tattgagtcg
     4621 ttattcttca aacgcaaagc atgggcgtgg tggaaatcga gagtcggtag cgagacagaa
     4681 gaagattcac cgttactgga tttgtatcgg gtagcggaac aggtagtact tgaagcgttt
     4741 ccgaaacaag agatgctggc ccgtcttaat acaaggctgg aaggcggaga tgctcatcca
     4801 ttagaggcca ttatcacccg gatgctgttg acgaaagtaa aactcgagcc ggaggatgaa
     4861 gatgtcgatg agccaacacc tgaaaaccat gaagaaaaaa atgatgaggg tgaaaaacca
     4921 cagagcatta ccagcattat caaaatcagt ttaacggtgc tggttatagg ttatgctctc
     4981 ggcaaaatcg cgatgttgtt tagttgacga tgtgtttttt gcgccgatgc cggatgcagc
     5041 atttacgccg catccggcag ccgtgattat gaggtctcaa tggctaaata aacgctcgcc
     5101 cgccgcctga cctcatcgac gcgggccata tcgtttgact gcaacgccgc atcaaaatcg
     5161 gtgatcaccc ggccaatctc ttctcgctcg tcacccagcg cccgcgccca caactcttcc
     5221 agtttggcct taaaggtgcg gttgatgagc atatcgcgcg gataaatttt caatgcggat
     5281 aaccgcgtcc gactctcttc aatctgctgc gcgctcaatg ttaccgggct atgattaatg
     5341 actctggact taacgctgcc atcttccaga agcacgtcaa cttcaagcaa gccgttgata
     5401 tcataactaa agcgaatatc aatcgactga taagccccgg ttttcttcaa cggcacatcg
     5461 aaggattcaa ccagaatgtt atttttaact ttgtggtttt ctccctgata gacgttaacc
     5521 gtaattgaat tctgttccgg gtgcatggtc gagtaagttt ctacacgcga cacgggtact
     5581 gtggtgtttc gttcaataat tggcgagaaa atgccggaaa cgccctggcg gttaacttca
     5641 acgcccaacg agtaagggca aatatcagtg aggatcacct cttcaatatc ttcactgcgt
     5701 aagcggcagg cggcctggat tgctgcgccc agcgcgacaa tggtgctcgg gtcgtaactt
     5761 tgatacggta atttgccaaa cagacgcacg gcgattcgct gcaccagcgg catttgtgac
     5821 gcgccgccaa ccagcaccag actgtcgatt tgactcggct tcagacgtgc atcgcgcaac
     5881 gcctgttcaa tcggcactcg caagcgattg agcagcggca accacaaatc ttccagttcg
     5941 ttctcgtaaa attcgcactc ccgcgtttca tcctggtact gccagcgaat gtgcaatggc
     6001 gattgattgc tacattttgc cgcttccaca caggcgtaca aggctcccag ttcactctcg
     6061 ttaagcgtgg tcctggcgac atccgcgcgt tttaaaacct catcgaccag catatgggta
     6121 aaatcttcgc caccaagaaa gttgtcgcca gcggaggcgt gaacttcaat caccggcgtg
     6181 gcgtactcaa gcaccgtaac gtca
//
LOCUS       DAZFDP010000228         6114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFDP010000228 DAZFDP010000000
VERSION     DAZFDP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..240
                     /locus_tag="ACHJ1G_004772"
     CDS             106..240
                     /locus_tag="ACHJ1G_004772"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766483.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            841..1662
                     /locus_tag="ACHJ1G_004773"
     CDS             841..1662
                     /locus_tag="ACHJ1G_004773"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4766484.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1662..2768
                     /locus_tag="ACHJ1G_004774"
     CDS             1662..2768
                     /locus_tag="ACHJ1G_004774"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4766485.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2862..4583
                     /gene="cptA"
                     /locus_tag="ACHJ1G_004775"
     CDS             2862..4583
                     /gene="cptA"
                     /locus_tag="ACHJ1G_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4766486.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4657..5655
                     /gene="lpxM"
                     /locus_tag="ACHJ1G_004776"
                     /gene_synonym="msbB"
     CDS             4657..5655
                     /gene="lpxM"
                     /locus_tag="ACHJ1G_004776"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4766487.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5708..5896
                     /locus_tag="ACHJ1G_004777"
                     /pseudo
     CDS             5708..5896
                     /locus_tag="ACHJ1G_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5959..>6114)
                     /locus_tag="ACHJ1G_004778"
     CDS             complement(5959..>6114)
                     /locus_tag="ACHJ1G_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4766488.1"
                     /translation="RGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPW
                     RIALPAE"
BASE COUNT         1553 a         1343 c         1553 g         1665 t
ORIGIN      
        1 gctttttaaa ctaatatcga ctttcaataa ataaggacag gtaatggaca acaaaaatat
       61 ctgaacccgg gtgagattca taagttgcac agtgctgtct tcaaagtgcc acacccggaa
      121 cgtaatcact gcctgctcct tatggggtat cttcatggtg tacaagcatc agagttatta
      181 ggtataaaac tgtctgatat tgatttgcag gccggtaacc tgaatatccg gcggctctaa
      241 acggtttgtc aactcagccc ctcggctgaa acaggaggta cagtccattc gtcgctggct
      301 gaaacttcgg gatgaatggg tagaagaggg atatgaatgg ttatttaacc tctatggcag
      361 gtctgctaca ggtattactg ttgttctggg gggcactggt gtggttaata tctacgatgc
      421 aagagattct cacgatggcc cggtccatca gtgcatgcgg cgcgatattg tgcgagtatt
      481 tttcaggggg agtggtatcc ggatgcagaa aattccgcta ctcgactggt tttatgcaat
      541 aaatcaggat gataatcttc tgatttttta ctgaggcgga tatctctcgc tgtgtttact
      601 gaaattgcgg tattctgttt tttctgaaaa acagaaaatc tgattaaaaa gagcatatgc
      661 gttaagggaa atttaagttc tggttgatta actgataaaa atattaaaaa atccctgttg
      721 tcggtaatct ccggaccatt ccgggattac acaataatat caccatgtgt gatatctatg
      781 gatcctcact gttgtttacg cgaggatatt ttcagaatgc ttaacagacg ataatttttt
      841 atgttgaatg caaggcatct tcccgtactg atgtatcatc atgtcagtcg ttgtccgggg
      901 cttgtgacac tctcacctga aacattttgt gaacagatga agtggctcgc agataataac
      961 tggcggacgg taacatctgc tgagcttgaa tatttttatc aggggggaac gcttccacga
     1021 aagagtgtca tgctcacatt cgatgatggt tatcttgata actggctcag agcatggccg
     1081 gtcctgaagg aatataacct tcatgcacat atttttctca ttacggggct gattggtaag
     1141 ggaaacgtac gtagcaggca ggagcaggaa tattctcacc gcgactgtga acggttgatt
     1201 gccgataacc gttcagatga ggtcatgctt cgctggtctg aagtccggga aatgcgtgat
     1261 tccggactgg ttgaatttca tgtgcacacc cattcacata aacgctggga caggttgtct
     1321 gtatcacgtg ctgaacagtg ccggctcatg aaagaagata ttctggtggg aaaacaatgt
     1381 ctgacggaaa agctggggtt ctgtagttcc catctgtgct ggcctgaagg atattataat
     1441 cgggattata ttaatctcgc cggtaagctt ggtttctctt atttatatac aaccgaaaga
     1501 agaatgaact gtcctgaaaa tggttcatta cgcatagggc gtatcagcac caaagagcgg
     1561 gaacattccg gctggctgaa acgacgcctt ttttattata caacgccact tttttcttca
     1621 gtactggctc ttcataaggg gccaagatta ccagataatt aatgatgaaa attttattca
     1681 ctgaatcttc gtcagatatt gggggccagg agctgcaggc actggcccag atgactgctc
     1741 ttcaaaagca gggacattcc gttttacttg cctgcaggga aaaaagtaaa attgctccgg
     1801 aagccagaaa aagagggcat gatgtcactt ttattccttt caggaacagc cttcaccttc
     1861 cttccatcct cagacttcga cggattatcg gggaatttaa acctgatctg gtcatctgcc
     1921 atagtggcca tgacagtaat atagcaggac tgagccgtct gatatgctgt caccgcttca
     1981 gtattgtcag acaaaaaacc tacatcaccc gtaagacccg aacgttttcc ctgaattatc
     2041 tgtgtgattt cattgttgtg cctagctcag ccatgatggc gcatctgatg gcggaaggtg
     2101 tcaggacacc ggtaacggtt attccaccgg gtttcgactg gcccgcattg cataatgaag
     2161 ccatgcgacc gttacccctg catatccacg cctgggctgc atcagcagac aatgttccgc
     2221 ttatcgttca ggtggggatg ctacgtcccg aaaaaggaca tgaatttatg ctgcgtgtat
     2281 tgtatcagct gaagatggaa gggaaatcat ttcgctggct cgtggtgggc gcggggcgtg
     2341 aagaatacga agcacgtctg cgtcagcaga cagaacacct gggaatgagc ggagatgtgt
     2401 taatggcagg tgcccttttc ccagcgttac cggtctaccg gattgccagt gtggtggtca
     2461 tgccttcgga aaatgaagcg tttggtatgg tactggctga agcgtcagtc tctggcgtgc
     2521 ccgttatcgc cagtgagacg ggcgggatcc ctgatgtcat tcagaagaat gtgacaggga
     2581 cgctgttacc cgtgggggat gtgtctgcat ggaccggcgc actgcgcgat tttttatccc
     2641 gaccggaacg cttccggatg atggcagcca gcgccagaga agatattgag taccggtttg
     2701 atattaacag aacggcacag atcattgtgt ctctggctag tcaggctaag gggaaatgta
     2761 acagataacg gcggatcctc ttttcagccc gaattttgtt cagcggtttt ccggcaagta
     2821 acggagtaat ttatcacatt gtttaaatac ggggacggtc aatgcattta aataccggac
     2881 agaacaggcc tactttcagc tggagtgcgc tgggatgggc aattttttat tttggctttt
     2941 tttccactct cctgcaggtc atcatcttca gcagtgggta cagcggaacg aatggaatac
     3001 gggactcact gttattcagt tgtctgtggt tgatcccggt gtttctctat cctgatcgga
     3061 taaaaataat tgcagctgtt gtcggtttca ttctctgggg cacgtcgctg gcagcacttt
     3121 gttattattt tctctatggt catgaattct ctcaaagtgt tcttttcgtt atgtttgaaa
     3181 cgaatgccag agaggctggt gaatatttca gccagtattt cagccttaaa ctgttgctta
     3241 tctcgctggt atatactgcg gtgtccgttt ttctgtggac acgtctgcgt cctgtatata
     3301 ttcctttgcc atggcggaga attgtctctt tcctgctgct ttatgctctg cttctgcatc
     3361 cggttgttct gaaatcgtta atcagacagg agccgctgaa tgatactcta ggcaaactgg
     3421 catcccgaat ggagcctgct gccccctggc agtttgtatc cagttattat cagtaccatc
     3481 agcaactgaa tgcactgaca accttcctga atgaaaatag cgcactgcca ccactgggta
     3541 atctcaggga tgaatcaggg gagagaccac gcacactggt cctggtgatt ggtgagtcga
     3601 cacagcgcga acgcatgagc ttatacgggt atctacgtga aacgacgccg gagctggatg
     3661 cactgcgtaa aaccgatccg ggtcttactg tgtttaataa tgtggtggca tcgcgtccgt
     3721 acaccattga agcattgcaa caggccctta ctttcgccaa cgaaaagaat cctgatctgt
     3781 atctgacgca gccgtcgctg atgaacatga tgaagcaggc aggctataaa acattctgga
     3841 ttaccaacca gcagacaatc acagcccgta acaccatgct cactgtattt tcgcgccaga
     3901 cggacaggca gtactacatg aatcagcagc gaacacaaag tgcgcgtgaa tatgacacta
     3961 acgtgctgaa gccgttccgg gaagtgctga atgaccctgc accaaagaaa ctgatcatcg
     4021 tgcatctgct gggtacgcac attaagtata aataccgtta tccggaaggt caggggcggt
     4081 ttgatggcat tacagggcat attcccactg gattaaatgc gaaagagctg gaagtgtata
     4141 acgattacga taatgccaat ctgtttaacg atcatgtggt ggccagtctg ataaaggact
     4201 tcagagcaac tgcgccggac ggctttctgc tttatttttc agatcatggt gaagaagtat
     4261 atgatactcc gccatataaa acgcagggac ggaatgaaga taaccccaca cgtcccatgt
     4321 acactgttcc gttcctgctg tggacctcgg aaaagtggca tgctgcacat ccgcgagatt
     4381 tttcgcagta tgttgaccgc aaatacagtc tggctgaact gatccacacc tggtcagatt
     4441 tggcgggact gacatacgat ggttacgatc cgacccgttc tctggtgaat ccgcaattca
     4501 gggaaactac ccgctggatt ggaaatccgt ataaaaagaa tgggctcact gatttcgaca
     4561 ctcttccgta tggtgagcca tagtattttc ttatcttcgg ttccgttctg aaggcaggag
     4621 gatgtgtttt cctctgctgt ccggtggact ccagggatgg taccaccttc tgctgtactc
     4681 tgttatcata atgagataag caggcagata ccggtaaata tgaaaaatat cagaactgag
     4741 tttattcccc gctttaacct tacattatgc ttcccccgtt actggatgac gtggacaggt
     4801 atcggtatta tctgtgtgtt tgcaatggtc ccccccgcac tgagagatcc attgttggga
     4861 aaattgggca tgctggttgg gcgtctgggg aaaagcgcac gtcaacgggc cttgattaat
     4921 ttatcgcttt gttttccgga gtacagtgat aaagaaaagg agaatatcgt tgatgccatg
     4981 tttgcaacag cctccatggc cgttgtgctt atggctgaat tagcactgtc cgggcctgat
     5041 aaaatttcgc atcgtatacg ctggaatggt ctggagattg tggagaagat ggcacagaac
     5101 aatgaaaagg tcatttttct tgtgccacat gcctggggag tggatattcc cgcaatgctg
     5161 atagctgcgt cgggcagaaa aatggcagcg atgtttcata accagagaaa tccggtggtg
     5221 gattatgtct ggaacagtgt tcgtcgtcgt ttcggaggaa aactgcacgc acgtaatgac
     5281 ggtattgcct cctttgtccg ctcagtccgg cagggatact ggggatatta tcttcctgat
     5341 caggatcatg gcccggaatt cagtgagttt gcagattttt ttgcgacgta taaagcaact
     5401 ttaccggtta tcggacggct cagcaggatc agtggtgcac ggataatacc gcttttccct
     5461 gtttatgacg gaaagaccca tcatctgact attcatgtca gcccgccact tgctatacga
     5521 cagaagagtg atgctcatat tgcgcgacag ataaacgaag tggttgaaaa tttcgttcgt
     5581 ccacatcctg agcagtatac gtggatcctc aagttgctga aaacgcgtaa agaaggggaa
     5641 gaggatcctt actgaggcag gtttttcagg ataatgagct tacccagcaa tagtggacac
     5701 acgctaagtg agtaaactct cagtcagagg tggctcatat gacaaaaaaa cagtaccaac
     5761 cagtaaaaaa ccccgtaaat agcattcgcc tgaatttcgc agtgaagccc tgaagcttgc
     5821 tgaacgcatc ggtgttgctg tcgcagcccg tgtgctcagt ctgtatgaat cacagcttgt
     5881 aagcggctcg tcagaactgt attgatattt actgagcggt cgtaagcgta cagcgagggc
     5941 cgtattgacg gggatgtgtt attcagctgg cagtgctatg cgccacggaa gcagttcgct
     6001 gacccggttg accggccagt ctgctatgac gccaggcaca tggcgaaggt agctttctgg
     6061 atccacgtca ttcagtttgc acgtcccgat caggctgtac agtagcgctc cccg
//
LOCUS       DAZFDP010000229         6090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFDP010000229 DAZFDP010000000
VERSION     DAZFDP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..824
                     /gene="exuR"
                     /locus_tag="ACHJ1G_004779"
     CDS             48..824
                     /gene="exuR"
                     /locus_tag="ACHJ1G_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4766489.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHJ1G_004780"
     CDS             1064..1147
                     /gene="yqiM"
                     /locus_tag="ACHJ1G_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4766490.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHJ1G_004781"
     CDS             1169..1831
                     /gene="yqjA"
                     /locus_tag="ACHJ1G_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4766491.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHJ1G_004782"
     CDS             1835..2218
                     /gene="mzrA"
                     /locus_tag="ACHJ1G_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4766492.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHJ1G_004783"
     CDS             2365..2733
                     /gene="yqjC"
                     /locus_tag="ACHJ1G_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4766493.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHJ1G_004784"
     CDS             2771..3076
                     /gene="yqjD"
                     /locus_tag="ACHJ1G_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4766494.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHJ1G_004785"
     CDS             3079..3483
                     /gene="yqjE"
                     /locus_tag="ACHJ1G_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4766495.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHJ1G_004786"
     CDS             3473..3772
                     /gene="yqjK"
                     /locus_tag="ACHJ1G_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4766496.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHJ1G_004787"
     CDS             3958..4350
                     /gene="yqjF"
                     /locus_tag="ACHJ1G_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4766497.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHJ1G_004788"
     CDS             4420..5406
                     /gene="yqjG"
                     /locus_tag="ACHJ1G_004788"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4766498.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHJ1G_004789"
     CDS             5699..6064
                     /gene="yhaH"
                     /locus_tag="ACHJ1G_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4766499.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1519 a         1508 c         1541 g         1522 t
ORIGIN      
        1 taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg gaaatcactg
       61 aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatc gaacagggcg
      121 tctatctggt aggtgataaa ctgcctgcag aacgctttat tgccgatgaa aagaacgtca
      181 gccgtacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat gtcgaagtgc
      241 gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag gcggctgaca
      301 ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc cagctcatcg
      361 aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc atgaaactga
      421 tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag tgggatttac
      481 agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc atcgttgaaa
      541 aaatgtggac ccaacgtagc cataacccgt actggaaaaa actgcacgaa cacattgatt
      601 cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg ctgattcgca
      661 aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc aagatcatgt
      721 tattcaacga aaccagcgac gacttcgagt tcaatgccga ccgctatctg ttcgccgaaa
      781 acccggtagt gcatctcgat acggccacca gcggcagtaa atgaaatacc tgtccgacag
      841 gcgctgcgac gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg
      901 taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg acatgtaatt
      961 ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt tatgtttttg
     1021 aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga tgtatcaaac
     1081 ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa aaccagcgtg
     1141 gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc tgcaagccct
     1201 gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt tgtattttgt
     1261 cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt taccgggcga
     1321 cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac
     1381 gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata ttcaggggcg
     1441 atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg cgcattatca
     1501 tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt
     1561 tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc tgaataacgc
     1621 gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc tgacaactct
     1681 gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc tgatgtcatg
     1741 cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt
     1801 atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca tgtcgcttcg
     1861 ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc tggcctggtc
     1921 cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag gcacaacgat
     1981 gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt tcaaaagtat
     2041 cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga gcgccgccgc
     2101 aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc aggacaataa
     2161 cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct tcggataact
     2221 tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt ctatgattaa
     2281 tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc
     2341 cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt ttctctcttt
     2401 gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca aaatatcctt
     2461 aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg tctgaataaa
     2521 gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga tcatcacaag
     2581 aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc cgaggcgaag
     2641 caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga agcgcaggaa
     2701 gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac ttactcacct
     2761 ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt tgaaatccct
     2821 ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag aagagttgag
     2881 taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc tgggtgaaac
     2941 cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg agtatgtgcg
     3001 tgaaaatccg tggaccggcg tgggcattgg cgctgcaatc ggtgtagtgc tcggcgttct
     3061 gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg taaaagcgtt
     3121 ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac acgtctgcgg
     3181 ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt actgatgctg
     3241 ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct aattatttgg
     3301 gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt gttgctgcta
     3361 ctggcactaa ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac cttgctgcgc
     3421 catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc ccgtgagcag
     3481 taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc aacaacggct
     3541 ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg atcgtcgctg
     3601 gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga tggcgatctg
     3661 gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg gcgtatggag
     3721 cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt aacttccctc
     3781 tggccggagc aattccggcc ttatccctca aatttcttga agatttttga cagttttcct
     3841 tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa cgacgctaat
     3901 accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga gagtatgatg
     3961 aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat tctgtttatt
     4021 accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat ggaagcaatg
     4081 ggtgtcccgg gttttatgct gccgctggtg attctgcttg agttcggtgg tggtctggca
     4141 atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt tacgctgctg
     4201 acggcatttt tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa
     4261 aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc
     4321 atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga
     4381 ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact gattgacggc
     4441 gtctggcatg acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct
     4501 tccgcatttc gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt
     4561 tttatcgcag agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac
     4621 cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg
     4681 aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga
     4741 gatacgctct atcaacatga atttctgtat cagctttatc tccacgccga tccacactac
     4801 agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac
     4861 gaatcagcag aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg
     4921 ggtgattact acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat
     4981 gacaccgtta acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac
     5041 gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat
     5101 cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac cacgctggtc
     5161 cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac
     5221 cttaatctgt atggcttcct gcgtgatatc taccagatgc cgggaattgc cgaaacagtc
     5281 aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg
     5341 attatttcaa ttggtccatg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc
     5401 ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat
     5461 gtcggatgcg gcgtgaacgc cttatcaaac ctacaaaaca tttatattca atatgttgca
     5521 atgaccgcgc aggccagata agcgtagcgc atctggcgat tttgcgcttg tcatcagtct
     5581 tagcgcgcct tcacagcact ttattaattc cttgaacgaa tatttactgc catttcattc
     5641 acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat
     5701 ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa
     5761 agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga
     5821 taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc ctgacgacaa tctacggtat
     5881 tctggtgttt ttaccgtggt gggcagttca gttccgccgc ctgcacgaca ccgaccgctc
     5941 ggcgtggtgg gcactgttgt tcttaatccc gtttatcggc tggcttatta tcatcgtctt
     6001 taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga aactggaacc
     6061 ttaaaacaaa tctgctcgcc ggatgtctga
//
LOCUS       DAZFDP010000230         6063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFDP010000230 DAZFDP010000000
VERSION     DAZFDP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHJ1G_004790"
     CDS             <1..261
                     /locus_tag="ACHJ1G_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4766500.1"
                     /translation="LAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYSLI
                     GTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
     gene            300..653
                     /locus_tag="ACHJ1G_004791"
                     /pseudo
     CDS             300..653
                     /locus_tag="ACHJ1G_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(693..1163)
                     /gene="yehS"
                     /locus_tag="ACHJ1G_004792"
     CDS             complement(693..1163)
                     /gene="yehS"
                     /locus_tag="ACHJ1G_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4766501.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(1210..1929)
                     /gene="btsR"
                     /locus_tag="ACHJ1G_004793"
                     /gene_synonym="yehT"
     CDS             complement(1210..1929)
                     /gene="btsR"
                     /locus_tag="ACHJ1G_004793"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4766502.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1926..3611)
                     /gene="btsS"
                     /locus_tag="ACHJ1G_004794"
     CDS             complement(1926..3611)
                     /gene="btsS"
                     /locus_tag="ACHJ1G_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4766503.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3833..3898
                     /locus_tag="ACHJ1G_004795"
                     /pseudo
     CDS             3833..3898
                     /locus_tag="ACHJ1G_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4126..4374
                     /locus_tag="ACHJ1G_004796"
     CDS             4126..4374
                     /locus_tag="ACHJ1G_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4766504.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNNGLTILRATDKESAHEFVQETLQEARETADDWFIH"
     gene            4996..5787
                     /locus_tag="ACHJ1G_004797"
     CDS             4996..5787
                     /locus_tag="ACHJ1G_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avirulence protein"
                     /protein_id="HFO4766505.1"
                     /translation="MGHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSPFYGSRG
                     LKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKFRADNRI
                     DAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYAAEHFGK
                     TWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKYVNTLDI
                     FTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT         1723 a         1488 c         1374 g         1478 t
ORIGIN      
        1 cttgctgaac gtcagcgaaa aacgaaaccg ctgttgaaat ccctggaaag ctggttgcgt
       61 gaaaagatga agaccctgtt cttcggctct ggccatggtg gtgagcgggg agcgctactg
      121 tacagcctga tcgggacgtg caaactgaat gacgtggatc cagaaagcta ccttcgccat
      181 gtgcctggcg tcatagcaga ctggccggtc aaccgggtca gcgaactgct tccgtggcgc
      241 atagcactgc cagctgaata acacatcccc gtcaatacgg ccctcgctgt acgcttacga
      301 acggcactga aattgccatt acctatgtct acaaaggtga caaggtgctt aagcaatctt
      361 ctgaaaccaa aattcaattt gcttctattg gtgcaaccac caaagaagac gctgccaaga
      421 cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg tgttgaagaa aaattaacct
      481 atacagatac ctacgcgcag gaaaacgtga ctatcgatat ggaaaaagtg gattttaaag
      541 ccctgcaggg tatttcagga atcaacgttt ctgctgaaga tgccaaaaaa ggtatcacta
      601 tggcgcaaat ggaactggtg atgaaagccg ctggttttaa agaagtgaaa taatcggttg
      661 gcggtcatgc tctaaacatg accgccaatt ttttagcctt ttttcacatg ctggcgcgct
      721 gccagtccac gcagaaaata acgtaaaaat tgatcgccgc attcgcggaa gtttttatga
      781 tccggtgcgc gcatcatcgc tgtaatttcc ggcatcgaaa cgcggaactg ctgttcggtg
      841 aggatagcca gaatgtcatc ggttttcagc gaaaacgcga tgcgtaattt tttcagcacg
      901 atgttgttat taatgcgacg ttccggctcc agtgccggag cagactcatc cttgccgcgt
      961 ttttcataaa tcaggccatt gaggaatgac gacaaaaaaa tgtccggaca acgctgaaaa
     1021 ccctcttcgt cttctttacg tagccagacg gcgatctgtt ccgcggtggc ttcgacatta
     1081 cccagcgcca gaatacgcac caggtcatta ttattggctt tcaaaatgta gcgcacgctg
     1141 cgcagaatat cgttacttag catgaggcct tcaggtgttg atgaggcaaa aagccatttt
     1201 agcagtcttt tacaggccaa tcgcctcttt taagcttttc agataacggc ggctgaccgg
     1261 cacggttaag ccattacgca aaatcaactc ggcctggccg ttatcttcca gacgaatctc
     1321 ctgtaaatgc gcgaggttaa ccagatactg acgatggcag cgcagtagtg gtgtacgact
     1381 ttccagagta cgtaatgtca attcggtaaa gccctctttc ccttcgtggc tggtaacgta
     1441 gacaccgctc atccgactgc tgacaaatgc cacatctttc atttgcagca aataaatccg
     1501 actatgcccc gtacaaggga taaatttcag cgcctgttga ttttccggta acagcgaaac
     1561 atcctgcttg ctgcgctcct gacgcaatcg cgccagcgtt ttctccagtc gcgcttcatc
     1621 aattggcttc agcagataat caaaggcatg ttcttcaaag gctttaatag cgtattcgtc
     1681 aaacgcagtg agaaaaacaa tatacgggcg atgttccggg tcaagcatcc ccaccatttc
     1741 cagaccactg atgcgcggca tctggatatc gagaaacagc acatccgggc gcagtttatg
     1801 caccgcgccg atcccttcca cggcgtttga acactctcca acgatttcaa tatcgctctg
     1861 ctcctgcaaa aatacgcgca ggttctcccg tgctaacggt tcatcatcga caattaagac
     1921 tttaatcatg cctcgtccct ccatggtagt cgtaacgtta ttcgggtgta actatcaggc
     1981 tcacaggcga cgcttattcc atagtcatcg ccaaaccgtt cacgtaaacg cttatccacc
     2041 agattcatcc ccagcccact ggcattggtt accggttgat acaaaccggc attgtcttcg
     2101 atctccagca tcaaatgttg cccctcacgt cgggcgctga ttgccactcg ccctgtatcc
     2161 agcagttgtg atgtcccatg tttaatggcg ttttccacta tcggttgcag ggtaaacgcg
     2221 ggcaattgct gctgggataa ttcttgcgga atagcaatgt tgacctgcaa ccgcgactgg
     2281 aagcgcgcct tttcaatttg cagataagcg ttcacatgtt caatttcgtc ggcgagagta
     2341 acaaactccg aaggccgctt taagtttttg cggaaaaaag tggaaagata ctgcaccagc
     2401 tggctggcct gttcgctgtc gcggcggatc accgctttaa tggtgttaag cgcattaaac
     2461 aaaaaatgag gattcacctg ggcgtgaagc agtttgattt ctgactgggt gagcatcgct
     2521 ttttgccgct catattgccc ggcaagaatc tgcgccgaaa gcagttgcgc aatcccctcg
     2581 cccagcgtgc ggtttattga actgaataaa cggtttttgg cttcatacaa tttgatggtg
     2641 cccatcacgc gctgattttc gccacgcaac ggaattacca gcgtcgaccc cagtttgcat
     2701 tgcggatgca aagagcaacg ataaggtact tcgttgccat cagcgtagac cacttcaccg
     2761 gtttcaatcg cttttaaggt gtaagtcgaa gaaatcggtt tgccgggtaa atggtggtcg
     2821 tcaccaattc cggtaaaggc cagcaatttc tctcgatcgg taatcgcgac tgcaccaata
     2881 tccagctcct gatacagcac ctgagccact ttcatgctgt tcacttcgtt aaacccctgt
     2941 cgcaaaatgc cttccgtcga ggctgccact ttcagcgcag tggcagaaaa agcagaagtg
     3001 tatttttcaa acatcgcgcg tttatcgagc aatatacgca taaacagcgc cgcgccgacg
     3061 gtattggtga ccatcattgg tgcagcaata ttactcacca gacgcaccgc atcttcataa
     3121 ggtcgggcga tcgcaaggat gatcagcatt tgcaccattt cagcgacgaa cgtgacggca
     3181 ccggcggtaa tggggttaaa gactttatca gtgcgcccgc gacggatcag gatgctgtgt
     3241 accaggccgc cgagcaatcc ttcaacgatg gttgagatca tgcaacttag cgcggtcatg
     3301 ccccccatcg aatatcgatg taagccgccg gtcagaccaa ccagcccacc gacgaccgga
     3361 ccgccgagta agccgcccat taccgcgcct atcgcacggg tattggcaat agaatcgtca
     3421 atgtgcaacc caaaccaggt gcccatgatg cagaagatgg aaaagacgat gtagcagaga
     3481 aatttatgcg gcagacgaac cgtgacctgc attaacggta tgaataatgg cgttttactc
     3541 attaaccacg caatgactaa aaaaacgcac atctgctgaa gcagcagcaa caccagatta
     3601 aaatcgtaca tacccgcaaa ccacacttcc ctttaaaacg cgtaacatac attgcctgcg
     3661 tttaactttc tttgaactct tgcagaaaaa tgagaattcg tgagtacgat cactcaaaat
     3721 cgcctggcaa aaataaaatc accctataga tgcacaaaaa acgggcaaaa ctacctggtt
     3781 cgcaaaactg cgtctaaagt taaaccggga cctcgcgagc aagggtgaga cgatggcgct
     3841 ttacacaatt ggtgaagtgg cgttgctttg tgatattaac cctgtcacgt tacgcgcgtg
     3901 acgcaacatt tttaaaaatc aacgaagatc gtccaggagc gccgattttc aagggttgga
     3961 cagataattc gttttcgatg tcgacgaact tcgacggatt tcgcctataa aaagtgtccc
     4021 atacatgccc cattgatgcc ccatagacaa tatcagcaat tgacactggt tatctataca
     4081 actaaaaata acacctgtac ataaccacag ttacaggagg gtgttatgcg tgttgagagc
     4141 tgtatcgcta aagaaaaaat cagcaaaatg ccaaaaggtt ctgttcctgc cctgcaacag
     4201 gaaatgctgc gccgtatcag gaaacgatat gacaatgcag aaatcatcat caaatctgcc
     4261 agtaataatg gactcactat tttaagagcc acagataaag agtccgcaca cgaatttgta
     4321 caggaaacac ttcaggaagc cagggaaaca gcggacgact ggtttattca ctgactaaaa
     4381 tcccgtccac atgccgggaa tttcctatgt ccgaagaaaa tggtgtggtt aactaagcaa
     4441 aagtgtccct ttatcggtat agcaactcat tttgttaaca tacggttgca aaattgatga
     4501 caagtgttta ttttagccac aagaaatgta tgcaaaccag aattgggaaa tgcatttata
     4561 aaataaggaa tagcagatat gtttgcagca aagcccgaat taagtcaaag atgtatttct
     4621 ggactcctca caactgggcc aaatgatgaa aacgtaaata ataaaccgtc aagtgatatg
     4681 ccgaacagaa tcaaactagt tgtaagcgat tcacaaatcg gtatatatac aactgacagt
     4741 aatactctga aggatggatt gtgtagccaa gcccacatag gaactcaacc ttatcggcca
     4801 ggtgctgact cgccgataac tgtgagatca ttcctacaaa tcacactttc caacaataat
     4861 tacaaatcat tagagcatct gcaaacatga gcagagaatt atctgaggta tcgcaaagca
     4921 gaagagaata ttctgcgttc gaccgttgag gggctaacca atccagaatc tcctgtattc
     4981 aaacaaacag catggatggg gcatctggag cgtggccttt ggaaaacgga aacacgctgg
     5041 gatggaaacg accgcgagca gctaggcaaa gaggctcttg gctcagagga gccaaaacct
     5101 ggttcaccat tttacggtag tcgtggacta aaattatccg acagtgcgca ttctgccttc
     5161 tcaatgatgc tgtgcggttc tgaaggtcca ttcacaaaag aacaagcact ctctggtttc
     5221 gagcttgctc aaactggcca agtgctggct ggaaggctaa agattcagga gcgagttaaa
     5281 ttccgcgcag acaaccgcat tgatgctcag cgtaacggta cacactcaac ccgcacacca
     5341 acaggcatgg atctttctca agatatcggc acaattatgc gtgataaagc tgggctaccg
     5401 gtcatgagcg gaacatccgg ttcctcctct gatgccactc ttgcaacacg ttacgctgcc
     5461 gagcattttg gtaagacatg ggccgctcca ggcttaagcc aagcagaagg atgcaaagct
     5521 atctcggatc tgtcacatca ctattttcgt gcagaaggct ccagtccacc acaatcaatg
     5581 gcaacgggaa taaataaggt tcgttatgat gcaggaatgg aagagaaata tgtaaataca
     5641 ctggatatat ttacacatag ctacccagaa atttatgctg gggttgcatt gacgattgct
     5701 ggggctggcg gtaatgatga gcaggcaatg tataatgtta cgcaagaagc cgctcgtatt
     5761 ctgcacgaag ctgaaactaa agattgaaca ttctcaataa agagctgcgc caggcagcac
     5821 atattcagca ttgcagtaag atatgcatgc ccagccccct gtaaacctat aaatatttca
     5881 aatcatattg attatcttat agccatattt tttatgacac cctgtttcag tgaacatgaa
     5941 gacactcacc ttgttcctga tattcattag catcatcctg attatttaac ttctctattc
     6001 agggaagccc gtctgataca aagcttctat tcctcgttaa atattctcca ttctggcgcc
     6061 aag
//
LOCUS       DAZFDP010000231         6062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFDP010000231 DAZFDP010000000
VERSION     DAZFDP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..567
                     /gene="ygiL"
                     /locus_tag="ACHJ1G_004798"
     CDS             16..567
                     /gene="ygiL"
                     /locus_tag="ACHJ1G_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4766506.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            627..3131
                     /locus_tag="ACHJ1G_004799"
     CDS             627..3131
                     /locus_tag="ACHJ1G_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4766507.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3147..3896
                     /gene="yqiH"
                     /locus_tag="ACHJ1G_004800"
     CDS             3147..3896
                     /gene="yqiH"
                     /locus_tag="ACHJ1G_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4766508.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3898..4963
                     /locus_tag="ACHJ1G_004801"
                     /pseudo
     CDS             3898..4963
                     /locus_tag="ACHJ1G_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5006..5206)
                     /gene="glgS"
                     /locus_tag="ACHJ1G_004802"
     CDS             complement(5006..5206)
                     /gene="glgS"
                     /locus_tag="ACHJ1G_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4766509.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5475..>6062
                     /gene="yqiJ"
                     /locus_tag="ACHJ1G_004803"
     CDS             5475..>6062
                     /gene="yqiJ"
                     /locus_tag="ACHJ1G_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4766510.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSAT"
BASE COUNT         1853 a         1268 c         1215 g         1726 t
ORIGIN      
        1 atataaagga atattatgtc tgcttttaaa aagtcacttc ttgttgctgg cgtagcaatg
       61 attctttcca ataacgtatt tgctgacgaa ggtcacggca ttgttaaatt taaaggtgaa
      121 gttatctctg ctccatgttc aattaaacca ggtgacgaag acttaactgt aaatctgggt
      181 gaagtagcag ataccgttct gaaaagcgac cagaaatctt tggctgagcc tttcactatt
      241 cacctgcaag actgcatgtt gagccagggt ggaaccactt attcaaaagc gaaggttact
      301 ttcaccacag cgaatactat gacgggtcag actgacctgc tgaaaaacac caaagaaact
      361 gaaatcggtg gtgccacagg cgttggcgta cgtattctgg atagccagag cggcgaagtg
      421 actttaggta ctccggtagt aattacattc aacaatacta acagctatca ggaactgaac
      481 ttcaaagctc gtatggaatc cccaagcaaa gatgctaccc cgggcaacgt ttacgctcag
      541 gctgactata agattgctta cgagtaatca cattaggccg ggttcgcccg gccacatcat
      601 taacggatta atgataagtg gaacagatgt ataaaaaatt aaaattaacc acaataagcg
      661 aattgattaa aaatatttat tgttcattat ccgttctcat cattggttgt gcgtcagctt
      721 atgccgttga attcaacaaa gatttaatcg aagccgaaga tcgtgaaaac gttaaccttt
      781 cccaatttga aactgatggc caattacccg taggtaaata ctcattaaac gctcttatta
      841 ataataaaag gacgccaatc catctggacc tgcaatgggt attaattgat aaccaaactg
      901 cagtttgtct gactccagag caattaacat tattaggctt tactgatgag attattgaag
      961 aggctcagca aaacctgatc gatggttgtt accctatcga aaaagaaaaa caaattacaa
     1021 cttatctcga taaagggaaa atgcaattat ccatatctgc acctcaggca tggttaaaat
     1081 acaaagatgc aaactggacg cctcctgaac tttgggatca tggtattgct ggggcatttc
     1141 ttgactacaa tttatatgcc tctcattatg caccacatca gggcgataat tcacaaaata
     1201 taagttccta tgggcaggct ggggttaatc ttggggcctg gcgcctgcgt actgattacc
     1261 agtacgatca gtcatttaac aatggcaaaa gccaggcgaa caacctggat tttccgcgta
     1321 tttatttgtt tcgcccaatc ccagcaatta atgcaaaact aactataggt caatacgata
     1381 ctgaatcctc tattttcgac tctttccatt tttctggcgt ttcgttgaaa agcgatgaga
     1441 atatgttgcc gcctgatcta cgtggttacg caccgcaaat cacgggtgtt gcacaaacga
     1501 atgcaaaggt cactgtctca cagaacaacc gtattattta tcaagaaaat gttcctcctg
     1561 gcccatttgc tattactaat ttattcaata cattacaggg gcaactggac gtcaaggttg
     1621 aagaagagga cgggcaagtc acgcaatggc aagttgcatc taatagtatt ccttatctga
     1681 cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa accgaccagc gttggtggtg
     1741 attccttaca acaacccttc ttctggactg gtgaattctc atggggttgg ctgaacaatg
     1801 tatccctgta tggtggttca gttttaacaa accgtgatta tcaatctctg gctgccggcg
     1861 ttggctttaa ccttaactca ttgggttcat tatcttttga tgtcacacga tctgatgctc
     1921 agttgcataa tcaggataaa gaaacgggtt atagctaccg cgctaactat tcaaaacgtt
     1981 ttgaatctac cggtagccag ctcactttcg ctggttaccg tttctctgat aaaaactttg
     2041 tgacaatgaa tgaatatatc aatgacacta accattacac gaattatcag aatgaaaaag
     2101 agagttatat tgtcacgttt aaccagtatc ttgaatcatt aaggttaaat acatacgtaa
     2161 gtttggctcg taatacttac tgggacgcca gcagtaatgt gaattattca ttatcactta
     2221 gccgcgattt tgatatcggg ccattaaaaa acgtctccac ttctctaaca tttagccgaa
     2281 taaactggga agaagacaac caggatcaac tgtacctaaa tatttcgatt ccctggggaa
     2341 ccagtagaac attgagctat ggtatgcaac gaaatcagga taacaagatt tcgcatactg
     2401 cttcgtggta tgactcttcc gatcgaaata attcctggag cgtttctgct tcaggcgaca
     2461 atgatgaatt caaagatatg aaagcgtcac tacgcgccag ttatcagcat aataccgaga
     2521 acggtcgtct ctatctctcc gggacatcac agcgagacag ttattattct ctgaatgcca
     2581 gttggaatgg ttcattcacg gcgactcgtc acggagccgc tttccatgac tatagtggta
     2641 gtgctgactc gcgttttatg atcgacgcag atggtgcaga agatattccg ttgaacaata
     2701 aacgcgcggt aactaatcgg tatggcatcg gagttattcc atcagtcagc agttacataa
     2761 caacatcatt aagcgttgat acccgaaatc ttccagaaaa tgtggatatc gaaaactcgg
     2821 ttatcaccac caccttaacc gaaggtgcta ttggctacgc caaacttgat acccgcaagg
     2881 gctatcaaat catgggggtt attcgcctgg cagatggtag tcacccacca ctggggatta
     2941 gcgtaaaaga taaaaccagc cataaagaat taggactggt tgctgatggc ggctttgtat
     3001 acctcaacgg cattcaggat gacagcaaac ttactttacg ctggggtgat aaatcctgtt
     3061 ttattcaacc acccaatagc agcaacttaa ccaccggaac ggttatttta ccgtgtatta
     3121 gccaaaatta attcaggatg aaactaatgc gctacttaaa cacaaagaat ctcattgcag
     3181 caggagtatt actcgcctgc atgagcagca ttgcctgggg tgccatcatc ccggaccgta
     3241 cccgtatcat aatgaatgag tcagataaag gggaagcatt aaaacttact aatcaaagca
     3301 aaaatctacc ctatctggcg caaacatgga tagaagatac gaaaggcaat aaatcacgtg
     3361 actttattgt cacggttccc cctatggtac gtttaaatcc cagtgagcaa attcaaataa
     3421 gaatgataag tcaggaaaaa attgctcaac tgcctaatga cagagaaacg ctcttctatt
     3481 ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa tgtgatgcaa gtcactatgc
     3541 agcacgcatt gaaactattc tggcgaccaa aagctattga attagaagat gatggggtaa
     3601 tgtcttacga aaaagttgaa atcatccgta gaaatgatgg ttcgatccgc tttaacaata
     3661 agatgcctta tcacgttacg ctgggttata tcggaacaaa cggaatcaca atgttgccac
     3721 aaacgcaaag tttaatggta accccttata gccatgcaga tacgcagttt aaaaatgttc
     3781 cgtcagcttt tcaggttggt tatatcaatg attttggtgg attaagtttt tatgaaataa
     3841 actgcccgac ggttaataac tcttgtaatg tttccgtagc caaacgagat aaataatatg
     3901 cgctacttat taattgtaat tatatttatt ataggtttta gtgcattacc tgtatgggca
     3961 atggactgct atgctgaaca tgaaggggga aataccgtag tcattggtta cgtaccaaga
     4021 atttctatcc ccagtaatgg taaaaaaggt gataagatct ggcaaagcag tgagtatttt
     4081 atgaatgtct tctgtaataa tgcattacct gccccatcac ctggagaaga atacccatca
     4141 gcctggacta atataatgat gtttttacca ggcggtcagg acttttataa tcagaactcc
     4201 tatatttttg gcgtaacata caatggagtt gattatgatt cgactgcccc taatgctatc
     4261 gcagcaccag aatgcattga tataaaagga gcgggtacct ttaataatca ctataaaaaa
     4321 ccccgctgtc tgtagtggag gtcctgagcc tcaattatca gtaacctttc cagctcgcgt
     4381 acaactctat attaaattag caaaaaatgc caatagagtt aataaaaacc tggtgttacc
     4441 tgatgaatat atcgctctag agtttaaagg tatgagtggc gcaggagcta tagaagtaga
     4501 caaaaatttg accttcagga ttcgtggatt aaataatatt catgtccttg actgctttgt
     4561 taatgtcgca ctggaaccag ctgatggcgt tgttgacttt ggtaaaataa attcccgaac
     4621 aattaaaaat accagcgtta gtgagacgtt tagtgtagtc atgacaaaag atcctggtgc
     4681 ggcctgtact gagcaattca atattttagg gagctttttc actacggata ttttgagtga
     4741 ttatagccat ctggatatgg gtaatggact gctattgaag atatttcata aagatggaac
     4801 agcaacggaa tttaaccgct tctcacaatt tgcttctttt tcatcgtcta gtgcaccttc
     4861 ggtcaccgca ccattcaggg cagaacttag tgcgaatccg gcagagactg ttgttgaggg
     4921 accgtttagt aaagacgtca tcctcaaaat cacctataac tagcatctaa tacaaacact
     4981 aaaacgggcc atcaggcccg ttatatcagt gctctaactc cagctctctt gcctgcatca
     5041 tctgttgaca ataccaggca taacgtgcgc aaaaccaggt tctatgttct tcatccatgt
     5101 taccagtaac ggcaagaata tcgaccgggc gaccttctgc gtgcattctg gcaaaactac
     5161 gcgccaggaa atcgaaatta tttaaagaat taagactatg atccataagc acatcctccg
     5221 gttatcgcat accttttaag gccatatgct ttttctgatt tcagtattcc ccaatcagtg
     5281 tagtgccttt aaacataacg tgcgtaaata tattgtcccc gatcacactt tttagtgaaa
     5341 aataaaaaac catattaaaa aactttctct tccagaactt ctctaacatg tgttattatt
     5401 aacaaagata aatgtaagca aggaaactat cccaaagtaa atattgcgtc actaaatgga
     5461 cattggagtg acatatgatt ttattcgccg actataatac cccttatctt ttcgctattt
     5521 cctttgtact gttgatcggc cttttagaaa ttttagcgct tatctgcggt cacatgctct
     5581 ccggtgctct tgatgcacat cttgaccatt atgattccat aaccacaggt catattagcc
     5641 aggcacttca ttatctcaat atcggaagat tgccagctct cgtcgttctc tgcttgctgg
     5701 ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc ctgcatcatg gtctggcaat
     5761 caccactgtc gaacctgttc gtggttcctg tcagtttact gtttacgatt attgcggtgc
     5821 attataccgg taaaatagtt gcgccctgga ttccacgcga ccatagttca gctattacag
     5881 aagaagaata tattggtagc atggcattaa ttaccggtca tcaagccact tcaggtaatc
     5941 cctgcgaagg aaaactcacc gatcaatttg gccaaataca ttatttacta ctggagcctg
     6001 aagaaggaaa atttttcact aaaggagaca aagtgttaat tatttgccga ctttctgcaa
     6061 cg
//
LOCUS       DAZFDP010000232         6004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFDP010000232 DAZFDP010000000
VERSION     DAZFDP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..169)
                     /gene="hokA"
                     /locus_tag="ACHJ1G_004804"
     CDS             complement(17..169)
                     /gene="hokA"
                     /locus_tag="ACHJ1G_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO4766511.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(494..2563)
                     /gene="glyS"
                     /locus_tag="ACHJ1G_004805"
     CDS             complement(494..2563)
                     /gene="glyS"
                     /locus_tag="ACHJ1G_004805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4766512.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2573..3484)
                     /gene="glyQ"
                     /locus_tag="ACHJ1G_004806"
     CDS             complement(2573..3484)
                     /gene="glyQ"
                     /locus_tag="ACHJ1G_004806"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4766513.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3579..3878)
                     /gene="ysaB"
                     /locus_tag="ACHJ1G_004807"
     CDS             complement(3579..3878)
                     /gene="ysaB"
                     /locus_tag="ACHJ1G_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4766514.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4053..5048
                     /gene="wecH"
                     /locus_tag="ACHJ1G_004808"
     CDS             4053..5048
                     /gene="wecH"
                     /locus_tag="ACHJ1G_004808"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4766515.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLFLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5090..5527)
                     /gene="yiaA"
                     /locus_tag="ACHJ1G_004809"
     CDS             complement(5090..5527)
                     /gene="yiaA"
                     /locus_tag="ACHJ1G_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4766516.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5573..5914)
                     /gene="yiaB"
                     /locus_tag="ACHJ1G_004810"
     CDS             complement(5573..5914)
                     /gene="yiaB"
                     /locus_tag="ACHJ1G_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4766517.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1494 a         1525 c         1438 g         1547 t
ORIGIN      
        1 ttcccgccga agactcttac tcttttaaat tgcaggctaa aaatgccgcc agctcataac
       61 tcccctgttt aatatgcaat tcacacagtg aatctcttat catccaggtg aaaaataaaa
      121 gcgtgaaaca aatcactatt aaagaaagta atctatattt ctgcggcatt cccagccccc
      181 tgtgttgatt tccaacgagt aagaggctaa ccttatggtg ttcaggcata gggtagcctc
      241 actttgattt atagtcaggt ggggcttttc tctgtctgcc tttcggtgaa tacctgagac
      301 aaacagtctc aagcacccgt ggctattcta gcttaataag tttgtttctt ctccttgata
      361 taatcctaaa aaacctcata aaattaatat atgagataat ctttattcag cagaagatta
      421 ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt
      481 aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca
      541 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca
      601 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
      661 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
      721 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt
      781 cgtcagattt cgccaaaatg ttggagacac gcttgttcgc cgccgccagt gcagcagctg
      841 cttcaagggt acggaagtgc gataccgctt tcatgcgggc atcgaaatca gccggacgag
      901 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc
      961 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aaccacgttg gcgttagtca
     1021 gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt
     1081 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
     1141 tgtcgccttt cggatgctgg ccgataccga agatacccgc cagagtatcc atcttgtcag
     1201 caatcgccag cgcacaggct accgggttag acggcaggtc atcaccggca aagcgcggct
     1261 gatactgctc attcagcgcc accgcgacat cttccgcttc gccatcgtga cgcgcatagt
     1321 gcatccccat aacgccctgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
     1381 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa
     1441 tccagccagc cagcgcctgg atgcggtcag ttttgtcgcg cagcgtaccc agctgttgct
     1501 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat
     1561 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     1621 tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc
     1681 catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt
     1741 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     1801 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     1861 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     1921 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     1981 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     2041 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     2101 cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa
     2161 ccatattcgg cagcagtgct tcggtgcttt cgcccttcac atgggcgcga tacagcagcc
     2221 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg
     2281 cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc aaacgcctgg gcaatcgccg
     2341 ggccgcgttt ttcgatttca cgatccggtt gcgcttccgc caggttagct actttcagcg
     2401 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca
     2461 gctccgcagt aaaattcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca
     2521 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     2581 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg
     2641 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg gagatggctt
     2701 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     2761 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcaaagc
     2821 aggtgaacag gaagtccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     2881 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt
     2941 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     3001 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     3061 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt
     3121 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     3181 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     3241 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     3301 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     3361 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     3421 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     3481 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     3541 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     3601 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     3661 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     3721 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tactcaacga acgcagagga
     3781 tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc cactagcacc
     3841 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     3901 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     3961 tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     4021 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     4081 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     4141 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     4201 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     4261 gcgcccagcc gcgccatttc ttgcgtatcg gcttatttct gattttttat agcgcaatcg
     4321 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     4381 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     4441 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg
     4501 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     4561 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     4621 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     4681 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     4741 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     4801 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     4861 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     4921 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     4981 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     5041 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     5101 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggtatca cgaatattct
     5161 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     5221 cactgagtaa taatgtcgca ttccacagac ctaccatcag taatgcaaca gagataataa
     5281 agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca tacttgtccc
     5341 gaacggtctt ttgataagac gcagcagaaa acagtcctaa caccagtacg gcaaaataat
     5401 accctttttc atttaactgc atctctgcat tccatagccc taacagatag gtaacgatac
     5461 caccaacgag agctatccat gacacaatac taaaggccgg tgaataggtt gatattttgt
     5521 tgtccatcac agtattcctt tcattcctga atgtgtaaga gctttatgtt gctcataccg
     5581 agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca
     5641 tttcataagc attgataggt gcatttaaaa caccgataaa caagagaccg atagtgatta
     5701 acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc
     5761 gaagatatgc atagttacca aaagttgctg tcattaacac gccaagaaaa tagccttttc
     5821 cacttagcaa tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa
     5881 ataatagttt tgccacagtt tttgaggttt tcataacgat ctccatatct accagcgata
     5941 cattacgagt aaccaacgaa agacaaaact gaaaaatgcc attaacaaat gattttcaga
     6001 ataa
//
LOCUS       DAZFDP010000233         5971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFDP010000233 DAZFDP010000000
VERSION     DAZFDP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..1047
                     /locus_tag="ACHJ1G_004811"
     CDS             49..1047
                     /locus_tag="ACHJ1G_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4766518.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQY
                     QLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(1097..2845)
                     /locus_tag="ACHJ1G_004812"
     CDS             complement(1097..2845)
                     /locus_tag="ACHJ1G_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4766519.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISDSGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2845..3915)
                     /locus_tag="ACHJ1G_004813"
     CDS             complement(2845..3915)
                     /locus_tag="ACHJ1G_004813"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4766520.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            complement(3905..5356)
                     /locus_tag="ACHJ1G_004814"
     CDS             complement(3905..5356)
                     /locus_tag="ACHJ1G_004814"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4766521.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5367..5813)
                     /locus_tag="ACHJ1G_004815"
     CDS             complement(5367..5813)
                     /locus_tag="ACHJ1G_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4766522.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
BASE COUNT         1557 a         1511 c         1518 g         1385 t
ORIGIN      
        1 aattaaatga aaaggaattt attatcttcc gctattatag tcgccatcat ggccctcggt
       61 ctgacgggtt gtgatgataa aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa
      121 cctgccgcac cagctcctga agctcccgtt gcaaaagcag aagctaaacc tgaaacacct
      181 gcgcaaccgg tggtcgatga acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag
      241 tgctataaca agttacagat ccctgtacag agcagtctgg cgcgttatgc tgactggctg
      301 aaagatttta aacaggggcc taccggtgaa gagcgtactg tttatggcat ctacggcatt
      361 agtgaatcca acctcgctga gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct
      421 gcgctgcaac caattgatgg cgtggcggtg agttatatcg atgctgccgt ggcgttgggt
      481 aataccatta acgaaatgga taaatattac acccaggaaa attataaaga cgatgcgttt
      541 gcgaaaggga aaacgctgca ccagacattc ttaaaaaatc tggaagcctt tgaacctgta
      601 gcagaatctt atcatgcggc gattcaggaa attaacgata agcgccagct tgcggaactg
      661 aaaaatattg aagaaagaga agggaaaaca ttccactact actctctggc agtcatgatt
      721 tcagcgaaac aaattaataa cctgatatcg caagagaagt ttgatgttga tgcggcaatg
      781 aagaaagtgt ctgaactgga aacgctagtg gcgcaggcca aagaagcgga taaaggcggc
      841 atgaatttct cgtttattaa ttcggcaggc cagtatcaac tcgaggctaa aaaatacgtt
      901 cgccgcatca gagataaagt cccgtactct gactgggata aagagcaact tcaggacgca
      961 aactcaagct ggatggtcga agactctttc ccgagagcat tacgcgagta caacgaaatg
     1021 gttgatgact ataatagcct gcgttaaacg tttttgatga taaatgcagt gtcggatgcg
     1081 gcgcatccga cacagttcac cccttgtgaa gtaactccat aaatgcctca tatcctgtta
     1141 acccggcaat aatctccgcc ggatgctgac acaatatgct tgccagatag ctaaatattt
     1201 ttagctcatg acgcgcatcg gcggcggcgc aggcgatcaa gacatggtta atgacttcgt
     1261 tattcacctc aactggttgg gcgagggtaa taaaaaatcc acgaaaacgt cgctcctgtt
     1321 cgctccagca atgtgggatg gcgaggcgat taacaatcag gttttcacct tcgccttcgc
     1381 gggcgatgat gcgttgtgct tcatcggcgg taatatggtg ttgtgcgact aattgcgcac
     1441 aaatttgccg ggtaatatgt tgccagcttt caccgcgtac attcgaataa tgaaaacttc
     1501 cgggagcaga gaaaaaacgc tccggctgtt ggcgaataaa tgccgtcgcc agaaaatgtt
     1561 ttatttgttc gataccggca gccgtaatga tattttttac ggtgatgtaa ttatttaccg
     1621 cgtcatccag taaataatgg ctgttgttaa tgatcaataa cggctcaatc tcttcgcgaa
     1681 tggcaacaag ttcgcttaag ctacgggcaa taatcacccg acaatgtaaa acatcgcgct
     1741 caatggcgag ctgattaata gtggcaatcg cgttctggtc cgagagcaaa atgatcggct
     1801 ggcgttcgtt ttgatgccgt tccagcgcac aggcaaaata caaaccgatc agatcgctgt
     1861 cgaaaagggg aatatcgagc tgttcacgca gtagcgtaat aaagtgcaga ctcatatcaa
     1921 aggccgcagg ccaggcggct ttcaggttat tcatgctgct ttggcggtgc tcagcaatcc
     1981 acaccggtgc ggcaaggcaa cgtaataaat gcccggtaat gttctcaacc agctgcgtat
     2041 caataatatt caatgacgcg atgcccggca ccctttgcag caaccgctgc acattatcgg
     2101 cagaaattat ctgcgcttgc tgatgctgtt tctctatcaa acctgtcagg gttcttacag
     2161 cgttatcacc aagaaacaga ccgctatgtt caacgatttg ttttatctcg tcaccgggat
     2221 attgcggcca ctcatcggtc agttgattac gcagggcgta aatcgccaga atcaggctgg
     2281 agagataatc accctgcatg agcggccagc ggtgttggtt gtcgcaggcc gtggataaat
     2341 gctgaaggtt gtctcgtgtt acgcccgcca gcggaattaa aaacggatct ttgcgcagca
     2401 agttcgccag caatataacg cgtttttctt ctgtctcatc aatgaaatgg cccaaaccgg
     2461 ggcgactggc caggcagcaa gtgcgttcat aacgctgttt taaccggggc agacgctctg
     2521 cgacccacgt ttccggcaaa ttaagcgccg aggcgagttg cgcacgggga gtaaaggtat
     2581 tcagtaataa cagtgccagc agccgatcgt cgttatcgtg cttttgcagc aactgaaaaa
     2641 agctgcggcg ctcgaagatt tccagctgat agcccgcact accactgtca gaaatgcggg
     2701 ctttgccgtt aagggtaaag ttgagatagt caatatcacg cagtatggtc ctgccagaaa
     2761 cgccagtctg ttgcgccagc tcgccaggcg tgcgcggctg ttgctccagc agatcgacaa
     2821 tctttaactg gcgttcgttt agcattaatc cacctgacgg aactggctaa acgcgctgac
     2881 tttttccgcg gttaaggtgg tcagaaaacc ccgtatcagc gtgagcagag catcgtaatc
     2941 ggcttttgaa atcatgccgc tgttggcgtg cagataacgc gttggcaaac acagcgcgac
     3001 aaccggacgc ccaccgccca tcacgttgta gcgcccgcca tccgtcgcac cggttttcat
     3061 ggtggaaaat tgcagcggta aatcattctg cgtggcacag ctttttaacg ctgctaccag
     3121 tttctggttg gggaagtagc gcttgtcaaa cagcatcaac cccggcccgt tgcccagttt
     3181 cagcggatat ttaatgttat caatgcctgg aacatcgccc gcgacagcgg tgtccagcac
     3241 gatcaccaca tccggtttaa tgtgttcagc cgaggtttgc gccccgcgta gcccaacttc
     3301 ttcttccaca ctgccaacgc catacagcgt aatttcggga ttattcaccg tctgtagtag
     3361 ctcagccatc attgcgcagc cgatgcggtt atccaacgcc ttgccgacca ctttatcttc
     3421 gccccagcag gcaaaattgg cttccgggct aataaaatcg cccatcgcca caccgcgttt
     3481 ttccacctca tcgcgactgt ttgcgccaat atcaatgaac atctcatcaa atgactgcgg
     3541 ctgttgcttt tgcttttccg ttaacgcatg aggcgcgacg gaaccaatca caccagggat
     3601 tttcactccc ttgtgtgtgc gtatggttac ccggtggttg agcatcgact gattccacca
     3661 gccaccaatg gtggtgaaac gcagaaaacc gctctcgtcg atgtgggtga ccataaagcc
     3721 gacttcatcc atatgcccga caacggcaac tttcggccct ttatttccct tacgggcgac
     3781 aaagctgccc agcccatcaa aggtgatttc attcacgcaa ggttccagcg tgtttatcag
     3841 aatgtcgcga acttcctgtt catcgccgct aacggcgctg gcttcgcaca actgttgcag
     3901 taactcaatg ttcatggaca agctcctgtt gcgcggcttt cgctttgcgg cgtaaccaca
     3961 atcctttcag gacgataatg aatgaaatgt tcagcgcgag gccaatcgcc agcaccagat
     4021 aaaacgaacc taccggtgac atcaggccga tcagcgggtc aaagataccc aaaccaggtg
     4081 ccagacgttt gattccgaaa gcgatcacca gcacgccggt aataccgccc gccagcgtat
     4141 tggcggtaat catcggcaac ggagctgcca gagcgtaagg aatagccggt tcggttgcga
     4201 ctgtggcacc gaccacaatg gcactactgg cagcctcttt ttcctgtcgg gtaaacagtt
     4261 tgggcgcaat gaacgtcgca agacccgccg cgacgggcgg catcagcgcg acaacgccga
     4321 cgatggcgta ccagtcataa atgtgttttt ccagcagtga gaagcagaag aaccaggcgg
     4381 ttttattgat ggggccgccc atatcgaacg ccagcatcgc accgaccaga aacgctgccg
     4441 caaacttcat tgatggagga atggtattca ggaagtggag cagcccgccc atgaggtcgg
     4501 acatcaccgg cccgataacg tagtaagtga gcacgccaaa aaccagcaga gtaacgaatg
     4561 ggataagcat ggagccgagc agcggttgta atgctttgcc aagacggact ttacggaacc
     4621 agaagacgaa atagccaatc gccagcccca gcaccaccgc gccgaggaag cccgcgcctg
     4681 actgggtgcc gagcagggct ttatcgttcg ccagatagca aaccagaaat gccggagcga
     4741 aagcgggttt atcggcaatc gacgaggcaa tataagcgcc catgatgggg atcataaagg
     4801 tgaagccgag gtaaccgatg gattcaacta cccaggtaaa cgacggcgca cctttcgaca
     4861 tatcggtata tggcaaaccg aactgcacca gcatgttggc aagcgccacc agaataccgc
     4921 cgccgatgac aaatggcagc gccgcagaga cgcccgccat caggtggctc atcacgctgc
     4981 cgctttgcac ttcctgtttg ccgagcttca cgccgctatc tgcggcaaaa agctgcgagt
     5041 ttgttggtaa ttcgctgaaa atctggtcga tatttttcag cgcctgggag atggcaatct
     5101 cataaacttt cttcccggca aaccgcgccc ggtcatcacc gctcaggcca cgcccggtag
     5161 ctagaatgac gtaatcggca gcggcaatct cttcgctgga taagcggttt tcaacgccgc
     5221 tggacccttg agtttccact tttatggtat ggccgagaga acgggctttc tgttccaggg
     5281 cttccgccac catgtaggtg tgagcgatcc cggcggggca gttggtgatc gcgacaatac
     5341 gtaaggatga ctccatcgcc acttccttat gagctgaggg tttgatttaa caaagcaagt
     5401 acctggtcgg cgtcgccctg tcgcagttgg ttgataaaat cctgatggat aattttgcga
     5461 cacagtgtgc cgatgatttt gacctgatcc tcttcgccac tttgcggcac gccgaggcag
     5521 atccagcaat tgacgtcttc gccgtcgctg gctttccagt caatggcctg cgctttgcgg
     5581 gcgaataaca cgaacggttg tttaacgcag gcgcttttac cgtgcggcac ggcgatgccg
     5641 gaaccaaatc cagtagagtg cattttttcg cgcagcagca gggtttgcag gaactggtgt
     5701 gagtcggtga taaaaccgtt ttgtaacgcc attgtcgcca actgcttcag aatggaataa
     5761 gcgccattgc cctgaatatt caggtcaata cagcttgcag taagagctgc cattaatcgg
     5821 actccataaa atatatttta atcaattgta atggtggagc gattaagtga tgagaggtga
     5881 tatgtcggta aacaatgaca atttgagaca gagtgaaagg ccagatttgc ggagtaatgc
     5941 acataatggt tatttaaata aaccacatga a
//
LOCUS       DAZFDP010000234         5915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFDP010000234 DAZFDP010000000
VERSION     DAZFDP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..288
                     /locus_tag="ACHJ1G_004816"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHJ1G_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHJ1G_004817"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHJ1G_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO4766523.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHJ1G_004818"
     CDS             complement(965..1639)
                     /locus_tag="ACHJ1G_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO4766524.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHJ1G_004819"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHJ1G_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO4766525.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHJ1G_004820"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHJ1G_004820"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO4766526.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHJ1G_004821"
     CDS             complement(3810..4130)
                     /locus_tag="ACHJ1G_004821"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO4766527.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHJ1G_004822"
     CDS             complement(4139..4441)
                     /locus_tag="ACHJ1G_004822"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO4766528.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHJ1G_004823"
     CDS             complement(4451..5230)
                     /locus_tag="ACHJ1G_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO4766529.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..5886
                     /locus_tag="ACHJ1G_004824"
     CDS             5611..5886
                     /locus_tag="ACHJ1G_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766530.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1332 a         1541 c         1592 g         1450 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgatataata
     5881 ccttagagaa tttctgtgta tgctattgtg aacat
//
LOCUS       DAZFDP010000235         5818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFDP010000235 DAZFDP010000000
VERSION     DAZFDP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /locus_tag="ACHJ1G_004825"
     CDS             complement(<1..184)
                     /locus_tag="ACHJ1G_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766531.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHJ1G_004826"
     CDS             complement(184..399)
                     /locus_tag="ACHJ1G_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4766532.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..940
                     /locus_tag="ACHJ1G_004827"
     CDS             542..940
                     /locus_tag="ACHJ1G_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766533.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1021..1179)
                     /locus_tag="ACHJ1G_004828"
     CDS             complement(1021..1179)
                     /locus_tag="ACHJ1G_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4766534.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1265..2008)
                     /locus_tag="ACHJ1G_004829"
     CDS             complement(1265..2008)
                     /locus_tag="ACHJ1G_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766535.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(2193..2882)
                     /locus_tag="ACHJ1G_004830"
     CDS             complement(2193..2882)
                     /locus_tag="ACHJ1G_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4766536.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(2897..3019)
                     /locus_tag="ACHJ1G_004831"
     CDS             complement(2897..3019)
                     /locus_tag="ACHJ1G_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4766537.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            3358..4317
                     /locus_tag="ACHJ1G_004832"
     CDS             3358..4317
                     /locus_tag="ACHJ1G_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO4766538.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(4461..4532)
                     /locus_tag="ACHJ1G_004833"
                     /pseudo
     CDS             complement(4461..4532)
                     /locus_tag="ACHJ1G_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(4529..4717)
                     /locus_tag="ACHJ1G_004834"
     CDS             complement(4529..4717)
                     /locus_tag="ACHJ1G_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO4766539.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(4714..5076)
                     /locus_tag="ACHJ1G_004835"
     CDS             complement(4714..5076)
                     /locus_tag="ACHJ1G_004835"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4766540.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(5073..5363)
                     /locus_tag="ACHJ1G_004836"
     CDS             complement(5073..5363)
                     /locus_tag="ACHJ1G_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4766541.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(5363..>5818)
                     /locus_tag="ACHJ1G_004837"
     CDS             complement(5363..>5818)
                     /locus_tag="ACHJ1G_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4766542.1"
                     /translation="LVDRWRELEETAVNIPKTLPEALRLAADLAEQKMQLENQLAIAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
BASE COUNT         1754 a         1376 c         1096 g         1592 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataacat tagtgatgta cgcatggcgc ctcccgctaa
      901 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
      961 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1021 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1081 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1141 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1201 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1261 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1321 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1381 aacgagcaag aacataatac caaccatcat tataattaat catttcatat tctttcttaa
     1441 actgtggttt gtaatatcct gtcagaaatg aaaaaagcca aaaatatgcc acaaaagcaa
     1501 tcatcacaat ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg
     1561 ataaaaatcg cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca
     1621 gtagcaaaag aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca
     1681 caaacaataa ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt
     1741 ttcttttcac tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa
     1801 taacgataac tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc
     1861 accatgggaa gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc
     1921 caaggcatcc catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca
     1981 taaccacccc aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat
     2041 gccgtcaaga ggttacaggc taaaaaaact ctattacata gcagccagca tgtttaccgt
     2101 acaagtacaa ctcagggcat aaaaaaaacc cactcggcag cgggtttata cattttttac
     2161 aacataccaa atttgcatga agtatatggc ttttaatcca gttttgcaat attttgctgt
     2221 aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa gcaataaagc atcactatca
     2281 agctgtagaa aaatgtgctt cattgcaacc cagcgttcag taaatgtctc ggaccagttt
     2341 tttgttgtca ctcccaccaa tgatgccagt gtctggtatt cataggcctc acgccctgca
     2401 agttcgctct tcacatcctg tgcagccagc cagattaacg tcttcaggcg atccagtgtc
     2461 ttacctgcaa tttttctgtt acttaacaaa tctttaaact cgctccatgc ccattgcgtt
     2521 atggtgacct gatgccccca tcgaacgctt tcgctgtaac accaaagcaa ccatgctttc
     2581 tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag aggttgagaa ttcaaccggg
     2641 ctgaccaaag caatggatga accttttgcg tacgactgct taccggaagt cggcgtatta
     2701 tccagcgtaa tcatcttgcc agttaccaca tccagaatgc gcggcttctt tcgtttgtat
     2761 gtaccagtat caaattgtgc atgctcctgc caggcttcga gctggccttt cgttgctccg
     2821 ttcaagtcag cagtagctgc cataagttgc tcacgaacat actgtaaata ttgggtattc
     2881 atgcagtaaa tcctttctat attttggcat aattcttcaa cattcggtaa tcgttcaaaa
     2941 ccgaatcggg gaaacgacat aagcacagga gcccccagcg acagcgaagg agttctgata
     3001 tataagactc agacatcatt cattccccgg ttctccaata tctgtttcac tcatcatcca
     3061 taactacctg taattgcccc cccttttttt gtaacagttc ttatattgct atatagaata
     3121 gccattacta atgcttttaa atttaataaa ataaaaatta taaaaaacat aaaacaccac
     3181 acaaacacac ttaataaaaa caccgttaca ttaaaagata ataaaaaccg caataaaaaa
     3241 cgaataaatc aattgtctca cgcaattata aaacatcata ttgattacgc accttgtatt
     3301 acaaactcat gtatgtaaaa tacgcgcacc attcaaaaaa aaggaagaca ataacatatg
     3361 aaaaaaagtg tcatcgctgg cgtctttatt gctctgtcat ttaccacgtg ttcagctatc
     3421 gcgaacagcc ttgcattatc attagcaaat gatgatgcag ggaagtttca accaatactt
     3481 aatgatattt atggcaataa acatgaaaac agagatgatt actcacaagg cttatttctg
     3541 ggatatagcc acgatatctc agactcgagc caattatctc tccatattgc gcaagatatt
     3601 tactctccat caggcagtaa taaaagacac aacacagctg taactggaga cagagctttt
     3661 agtgcataca ctcacactgg tattgaatgg aactcccttg cgaatgactg gattcgctat
     3721 cgattaggta ctgacatagg tgttgttggc cccgacgcag gcggtcagaa agtacaaaat
     3781 aaagctcatg agattattgg ggcagaaaaa tatcatgcat gggatgatca aatagagaat
     3841 cgctacggtt atactgtaaa agggatgcta tccatgacac caagtatgga tattttaggt
     3901 gctaatgttg gattataccc tgaagtttct gctgttactg gaaacttatt tcaatatgta
     3961 gcatatggcg caaccattgc cattggtaat gataaaacct tcaattcgga taatggcttt
     4021 ggtctgctgg ctccccgtgg tttaatgcat atgtccgata caagcggatt caaatacaag
     4081 atttttgcag gtatggaaag acgagatgtc aatcgcaact atactctcga aggaaaaaca
     4141 atacagacga aacaaacaac agtatcgcta aacaaaactg ttgatgaata tcaagttggc
     4201 gcaacaattg ggtatgcacc tgtagccttc acactagcat ttaataaagt aacatcagaa
     4261 ttcaagacag gggatgacta ttcatttata aatggagcaa tcaccttctt tttttaactg
     4321 aattgaattc aatcaaaata acataagtcc aacaaaaaca taaagtgcga aatgaatgcc
     4381 agctccattt atttcgcact ataaaagatt aaaagttgca ataaaataat aaaatgactc
     4441 agttacgaaa accaataaac tgtggccagt agtgagtcgc tcatcatcgg gctttttggc
     4501 gaatgaaatt tagctacgct ttcgagtctc atcgtcttcc cctcttgccc tgtttgacca
     4561 tcaggacgcc gttaactatt acatgacgct cgcctttgct gtctcggttg tacttgagca
     4621 ctgttcctct tgcgcaggaa agcatcctcg ccacttcggt ctgattgcct cgtgtctgga
     4681 taagaagctc tggtatcgtt tgaattgtgg cgttcatgcg ttctccagtt cggtgatttt
     4741 tattccaagc cgtccgcctg gtactttcac accacgaatt acgcgaatgt catcgaattg
     4801 ctcgtcgtct tccgcaaatc cggcatggat aagggagtcg agtaaaccct tcaggatgtt
     4861 atctaggtcg cggcggcggg agtctggaac gtctgcgatt actttgatgc ggagtcgtga
     4921 tttggtgaaa atatctaact tgagttggtg gatgatttgc tgaacgtctt ttcggtattt
     4981 ctggccttta tcgctgatgt agtattggct tccccgtctt cgccagtagg tattcaccga
     5041 cggcgggtat ggaagcacaa actgatattc gttcatgact taatcttccc ctccttcagc
     5101 agtatcgcct gcgtcctgat cacgccttcg aggtggtaaa gtctggcgtc tttgttgtcg
     5161 aggttatggg tgcgtcggtc gatttcatcg tgacacgcgc tacaagccca tgcgccgatc
     5221 aggtcgtcag gcttcattcc cgttccgcaa attccagcca tccggtaatg tgccagaact
     5281 gtagtttcag gattgccatt gcatatgccg taaatacgta cctggcattc tctgccgcga
     5341 gattctttgc gtaggttagc cattaagcag cctcccctgt tactttcagc attccgttat
     5401 cgagcagctt tctggtcagc cactgttgac cacgcccggt gatttttgtg gtgaacgata
     5461 tctgtattcc gtgatttgtg ttgaccgctg tttctttcac tgtgaaatag ccgcgatcca
     5521 tatattcctg cattggcaca ttgcgccggg aacctgaagc aataaggatt ttgtgatcgc
     5581 gcatccacgc aaacagtttg tttggaccaa taccaacaac ctttgcaaag tttccaatca
     5641 aaattccgct ggcctcgcca acgcgatcgg caaactcaac tttaggtgcg gcaattgcga
     5701 gctggttttc cagttgcatt ttctgctcag caagatcagc agcaaggcgc aacgcttccg
     5761 gtaacgtttt ggggatatta accgcagttt cttcaagctc tcgccaacgg tcaacaag
//
LOCUS       DAZFDP010000236         5779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFDP010000236 DAZFDP010000000
VERSION     DAZFDP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..861
                     /locus_tag="ACHJ1G_004838"
     CDS             <1..861
                     /locus_tag="ACHJ1G_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4766543.1"
                     /translation="DAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGKSGAG
                     KSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEA
                     LYRDILPELDLVLWLIKADDRALSVDEYFWRYILHRGHQQVLFVVTQADKTEPCHEWD
                     MAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAA
                     SPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSV
                     ARAVWNWIFF"
     gene            1233..4352
                     /gene="agn43"
                     /locus_tag="ACHJ1G_004839"
                     /gene_synonym="flu"
     CDS             1233..4352
                     /gene="agn43"
                     /locus_tag="ACHJ1G_004839"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO4766544.1"
                     /translation="MKRHLNTSYRLVWNHMTGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            4473..>5779
                     /locus_tag="ACHJ1G_004840"
     CDS             4473..>5779
                     /locus_tag="ACHJ1G_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766545.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISQGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCTSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDN"
BASE COUNT         1349 a         1538 c         1668 g         1224 t
ORIGIN      
        1 gatgccattg aggcaattga aaaaccgctc tcctccctgc cttactcact ttcccgtcac
       61 atcctggaac atctgcgcaa actcaccagt cacgaacccg tgattggcat tatgggtaaa
      121 agcggggccg gtaaatcctc actctgtaat gcactgtttc agggggaggt caccccggtc
      181 agtgatgttc acgccggcac ccgggaagtg cggcgcttcc gtctgagtgg ccacggtcac
      241 agcatggtta tcactgacct gcccggggtg ggcgagagcc gggacaggga tgcagagtat
      301 gaagccctgt accgtgacat tctgcccgaa ctggacctgg tgctgtggct gattaaagcc
      361 gatgaccgcg ccctgtctgt ggatgagtat ttctggcgat acatcctgca ccggggacat
      421 cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg agccctgcca tgaatgggat
      481 atggccggca ttcagccttc tcccgcacag gcacagaaca ttcgggagaa aacggaggcg
      541 gtattccgtc tgttccggcc cgtacatccg gttgttgccg tatcggcccg caccggctgg
      601 gaactggata cgctggtcag tgcgctaatg acagcacttc ccgaccatgc cgccagtccc
      661 ctgatgaccc gcctgcagga cgagctgcgc acggaatctg tccgcgctca ggcccgtgaa
      721 cagtttaccg gtgcggtgga ccggatattt gacacggcgg agagcgtctg tgttgcctct
      781 gttgcccgta cggttctgcg tgccgtccgt gacacggtgg tctctgttgc ccgcgctgta
      841 tggaactgga ttttcttctg aacagtctgc cagggaaatg cctgataaca ttacgttaac
      901 caactgaaaa catgacacaa acccctcata ctcctcctgt ttctcttgcg tgaccgcctt
      961 gtcgttaata gaataaaacg atcgataaaa caggtatcga tcgttgctat cgatcgttta
     1021 tagtggttaa tggtctgcga aaaggcagct gaatctcttc atcatgcaga acggaattgc
     1081 acacaacgga ctgatacttc tctggcggat gagagagggg aagcatttat gtccgagaaa
     1141 aaccacttta tctgagcatg ctgttttgta cctgccggta tccacatttg tgggtaccgg
     1201 cttttttatt caccctctgt aaggaaaagc tgatgaaacg acatctgaac accagctaca
     1261 ggctggtatg gaatcacatg acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac
     1321 ggggtaaacg tggcggtgtg gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg
     1381 tgctggctgc tgacatcgtt gtgcacccgg gagaaaccgt gaacggcgga acactggcaa
     1441 atcatgacaa ccagattgtc ttcggtacga ccaacggaat gaccatcagt accgggctgg
     1501 agtatgggcc ggataacgag gccaataccg gcgggcaatg ggtacaggat ggcggaacag
     1561 ccaacaaaac gactgtcacc agtggtggtc ttcagagagt gaaccccggt ggaagtgtct
     1621 cagacacggt tatcagtgcc ggaggcggac agagccttca gggacgggca gtgaacacca
     1681 cgctgaatgg tggcgaacag tggatgcatg agggggcgat agccacagga accgtcatta
     1741 atgataaggg ctggcaggtc gtcaagcccg gtacagtggc aacggatacc gttgttaata
     1801 ccggggcgga agggggaccg gatgcagaaa acggtgatac cgggcagttt gttcgcgggg
     1861 atgccgtacg cacaaccatc aataaaaacg gtcgccagat tgtgagaact gaaggaacgg
     1921 caaataccac tgtggtttat gccggcggcg accagactgt acatggtcac gcactggata
     1981 ccacgctgaa tgggggatac cagtatgtgc acaacggcgg tacagcgtct gacactgttg
     2041 tgaacagtga cggctggcag attgtcaaaa acgggggtgt ggccgggaat accaccgtta
     2101 atcagaaggg cagactgcag gtggacgccg gtggtacagc cacgaatgtc accctgaagc
     2161 agggcggcgc actggttacc agtacggctg caaccgttac cggcataaac cgcctgggag
     2221 cattctctgt tgtggagggt aaagctgata atgtcgtact ggaaaatggc ggacgcctgg
     2281 atgtgctgac cggacacaca gccactaata cccgcgtgga tgatggcgga acgctggatg
     2341 tccgcaacgg tggcaccgcc accaccgtat ccatgggaaa tggcggtgta ctgctggccg
     2401 attccggtgc cgctgtcagt ggtacccgga gcgacggaaa ggcattcagt atcggaggcg
     2461 gtcaggcgga tgccctgatg ctggaaaaag gcagttcatt cacgctgaac gccggtgata
     2521 cggccacgga taccacggta aatggcggac tgttcaccgc caggggcggc acactggcgg
     2581 gcaccaccac gctgaataac ggcgccatac ttaccctttc cgggaagacg gtgaacaacg
     2641 ataccctgac catccgtgaa ggcgatgcac tcctgcaggg aggctctctc accggtaacg
     2701 gcagcgtgga aaaatcagga agtggcacac tcactgtcag caacaccaca ctcacccaga
     2761 aagccgtcaa cctgaatgaa ggcacgctga cgctgaacga cagtaccgtc accacggatg
     2821 tcattgctca gcgcggtaca gccctgaagc tgaccggcag cactgtgctg aacggtgcca
     2881 ttgaccccac gaatgtcact ctcgcctccg gtgccacctg gaatatcccc gataacgcca
     2941 cggtgcagtc ggtggtggat gacctcagcc atgccggaca gattcatttc acctccaccc
     3001 gcacagggaa gttcgtaccg gcaaccctga aagtgaaaaa cctgaacgga cagaatggca
     3061 ccatcagcct gcgtgtacgc ccggatatgg cacagaacaa tgctgacaga ctggtcattg
     3121 acggcggcag ggcaaccgga aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt
     3181 cggggctggc gaccagcggt aagggtattc aggtggttga agccattaac ggtgccacca
     3241 cggaggaagg ggcctttgtc caggggaaca ggctgcaggc cggtgccttt aactactccc
     3301 tcaaccggga cagtgatgag agctggtatc tgcgcagtga aaatgcttat cgtgcagaag
     3361 tccccctgta tgcctccatg ctgacacagg caatggacta tgaccggatt gtggcaggct
     3421 cccgcagcca tcagaccggt gtaaatggtg aaaacaacag cgtccgtctc agcattcagg
     3481 gcggtcatct cggtcacgat aacaatggcg gtattgcccg tggggccacg ccggaaagca
     3541 gcggcagcta tggattcgtc cgtctggagg gtgacctgat gagaacagag gttgccggta
     3601 tgtctgtgac cgcgggggta tatggtgctg ctggccattc ttccgttgat gttaaggatg
     3661 atgacggctc ccgtgccggc acggtccggg atgatgccgg cagcctgggc ggatacctga
     3721 atctgataca caacgcctcc ggactgtggg ctgacattgt ggccctggga acccgccaca
     3781 gcatgaaagc gtcaacggac aataacgact tccgcgcccg gggttggggc tggctgggtt
     3841 cactggaaac cggtctgccc ttcagtatca ctgacaacct gatgctggag ccacaactgc
     3901 agtatacctg gcagggactt tccctggatg acggtaagga caacgccggt tatgtgaagt
     3961 tcgggcatgg cagtgcacaa catgtgcgtg ccggtttccg tctgggcagc cacaacgata
     4021 tgacctttgg cgaaggcacc tcatcccgtg cccccctgcg tgacagtgca aaacacagtg
     4081 tgagtgaatt accggtgaac tggtgggtac agccttctgt tatccgcacc ttcagctccc
     4141 ggggagatat gcgtgtgggg acttccactg caggcagcgg gatgacgttc tctccctcac
     4201 agaatggcac atcactggac ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca
     4261 ccctgggcgt tcaggccggt tatgcccaca gcgtcagcgg cagcagcgct gaagggtata
     4321 acggtcaggc cacactgaat gtgaccttct gacagaacca tcgcctctct gtggtcccgg
     4381 tcatcatgac cgggacccgg accggcgcaa cggatcttca acgccacatt cgctggcatt
     4441 aacaataaca tgatattcat cacggagtga ctatgttaca gatagtcggc gcgctgatcc
     4501 tgctgatcgc aggatttgcc attcttcgcc ttttgttcag agcattaatc agcacggctt
     4561 ctgcgctggc agggctcata ttgctgtgtc tgttcggccc ggccttactg gctggctata
     4621 tcaccgaacg cataacccgg ttgttccata ttcgctggct ggcaggcgta tttctgacga
     4681 ttgccggaat gatcatcagc ttcatgtggg gacttgatgg taaacatatc gcgctggagg
     4741 ctcacacctt tgactctgtg aaatttattc tgaccaccgc tctcgccggt ggtctgctgg
     4801 ctgttcccct gcagatcaaa aacattcagc agaacgggat cacaccagaa gatatcagca
     4861 aggaaattaa cgggtattac tgctgttttt atactgcctt tttccttatg gcgtgttctg
     4921 catgcgcacc attgatcgcg ttacagtacg atatttcacc gtcactgatg tggtggggcg
     4981 ggttgttgta ctggctggct gcattagtga cgctgctatg ggcggccagc cagatccagg
     5041 cgctgaaaaa actgacctgt gccatcagcc agacactgga agaacaaccg gtgctcaaca
     5101 gtaaatcgtg gctgaccagt ttgcaaaacg attacagcct tcctgactca ctgacggagc
     5161 gcatctggct gacgctcatt tctcaacgga tttcccaggg agagctgagg gaatttgaac
     5221 tggcagacgg aaactggtta ctgaacaatg cctggtatga aagaaacatg gcagggttta
     5281 acgaacagtt gaaagagaac ctgtcattca cacctgatga actgaaaacg ctcttccgga
     5341 accgcctgaa tttatcaccg gaagcgaatg acgattttct cgatcgttgc ctggacggcg
     5401 gtgactggta tcccttttca gaaggtcgcc gttttgtatc attccatcac gtggatgagc
     5461 ttcgtatctg tgcctcctgc gggctgacag aagtacatca tgccccggaa aatcataagc
     5521 cggatccgga atggtactgc acctctcttt gtcgcgaaac agaaacactg tgtcaggaaa
     5581 tttatgaacg cccttacaac agctttattt ccgatgcaac ggcgaatggt ctgattctca
     5641 tgaaactgcc ggaaacctgg agtacaaatg agaaaatgtt tgcttccgga gggcagggac
     5701 atgggtttgc cgctgaacgg ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac
     5761 ggatcctcgg tgataataa
//
LOCUS       DAZFDP010000237         5759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFDP010000237 DAZFDP010000000
VERSION     DAZFDP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..574)
                     /locus_tag="ACHJ1G_004841"
                     /pseudo
     CDS             complement(41..574)
                     /locus_tag="ACHJ1G_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(579..743)
                     /locus_tag="ACHJ1G_004842"
     CDS             complement(579..743)
                     /locus_tag="ACHJ1G_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766546.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(740..1473)
                     /locus_tag="ACHJ1G_004843"
                     /pseudo
     CDS             complement(740..1473)
                     /locus_tag="ACHJ1G_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1507..1968)
                     /locus_tag="ACHJ1G_004844"
     CDS             complement(1507..1968)
                     /locus_tag="ACHJ1G_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4766547.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1961..3001)
                     /locus_tag="ACHJ1G_004845"
     CDS             complement(1961..3001)
                     /locus_tag="ACHJ1G_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4766548.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2973..3524)
                     /locus_tag="ACHJ1G_004846"
     CDS             complement(2973..3524)
                     /locus_tag="ACHJ1G_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4766549.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3508..3735)
                     /locus_tag="ACHJ1G_004847"
     CDS             complement(3508..3735)
                     /locus_tag="ACHJ1G_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4766550.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3812..4219
                     /locus_tag="ACHJ1G_004848"
     CDS             3812..4219
                     /locus_tag="ACHJ1G_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766551.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4484..4783
                     /locus_tag="ACHJ1G_004849"
     CDS             4484..4783
                     /locus_tag="ACHJ1G_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766552.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4856..5074
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_004850"
     CDS             4856..5074
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4766553.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5097..5504
                     /locus_tag="ACHJ1G_004851"
     CDS             5097..5504
                     /locus_tag="ACHJ1G_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766554.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5482..5715)
                     /locus_tag="ACHJ1G_004852"
     CDS             complement(5482..5715)
                     /locus_tag="ACHJ1G_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766555.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1443 a         1502 c         1248 g         1566 t
ORIGIN      
        1 aaaataagga aaattatacg catattgaag agtataaacc ttacatgttg attacatttt
       61 tgtaatcaac atcctgtttg gaatagccag cctttaatgg ataactattt ctgacaatgc
      121 aatgagtata atcaagtcca tcttccactg agaattagag gcggcatgct ttttccggct
      181 cttgccggat atccgtaatt gtccataatc tgcagattta taccttctgc atgacctgtc
      241 agcgaaaatt tgtccggtgt ttctacggga atgacatcaa aagttacacg cactcgcgtt
      301 accgtgtaga cctactttcc tgcacttgca agatcacagt ggtgtaaccg taacaggaat
      361 ttattctctg gaccggcagt aaatccctga gtggcgtggt tcccatatca atttcccgcc
      421 aggcagcctc cattgccagc gtacaggctg gagccatgac ctgcccttta aatctggccc
      481 gaccatccca ccggacgtgt tcttctcccc tgaacttagg tacagtcatc tccagtggca
      541 caaaagtgtc agcgccataa tttttgaccg ttatcgcgct acggatattt tgttgactgg
      601 tgaaaatcac cccgcagaat caggcttatt cccttaacct gggctttcat cctgaccgcc
      661 gcctcactac gaccaatcag actgccgatg cattttacct tcattgttag tatcatgatt
      721 tcaggcctgc accatccgct cattgcccgg acttccgaca aatcccggca accatatccc
      781 ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc ccacttctgg acatgattct
      841 tcgccctacg cagttcgcga tttgccatat gcagcgatgg caaaatcaaa tcatcttctc
      901 gcgttgcagt aaacgatggc agcgactgca caatgtccgc cacagtttct gttttaatat
      961 cttcctgtgt tgcatctttc tgtaccggta acgaacaccg gctggctgag gaaaggcttt
     1021 accatcggtt tccgctaccg attcaacttt cggctctgct ggtaaattat cacctggtat
     1081 gcagtaacga attttaccgt tctggtttac gcgaatcaga cgacctttgc tgattgccat
     1141 tgccagcgtt gaagcaactt tgcgggatgt tgtaccgaac agcgtagcca gttcatccgc
     1201 cgtttgtggt ccgcgttgtt caatcgtcgc ggttaaatcg cactctgaga ttttcgcttc
     1261 tgttgccgtg gtggtttctt ctggcgctgg ctgttcctgc tgaacgttgt tatcagccac
     1321 acgccaggtg tatacgcttt tatcaacgaa gccagccttt ttcagttccc acagctcgtt
     1381 cagtacttct tcacgactga tatcaagtcg cgcagccagc tctaccgacg tggcttttcc
     1441 catcgctttc agtgcgtcaa aaacagtctc cataaatttc ctcccggtaa aaaatcactt
     1501 ctcaactcaa acaaaaccag ccgctttccg gcgttcatat tcctgtttca gcaactcaat
     1561 tggcgttggc cccgacgggc gtttgggggc cgccagttgt cgccggactg gcggaacgct
     1621 caggccgtta ctaacatgct ttgcccattt cgtcagttgc cgttctgcaa gccgttttaa
     1681 ttccccttcg gtcatctggc gctcaatccc ctttgaacgc atctcgaggc aaatgtgata
     1741 cagcacaggc tgagaccacg ggtacttatc acttccgtcg tatcgccagg actcattgcg
     1801 ccagcggcgg tactcctcca tcacagcatc caccgtcaga ccgaatggat tggctccgct
     1861 tttcgaaatc agtgccacaa actcagccag gtccggaggc catgtttcac ccgcccggca
     1921 gcggtccatg cactggcggc agacctgccg gatttgctgc tcagtcatcg cgccaatctg
     1981 tgcaatccag agcttcgaag gtgcggcccc gttcttctgg atccagcggt tcgaataaac
     2041 cacccccatg agttcccaca gcttccaggc cgtttccgtc gctgataaat ccgttttcac
     2101 gttcccactg ctcacgtgct gcccgaattt cctgaactgc ccgtgatgcg gtgccacctg
     2161 gtgctgctgc atggtttacc cctttgctga ctggtttaac ctgcgccctg acgtgattta
     2221 cgtgacgggc gaatttctgc tcccactgaa tctgcgtaaa cactttcccc tccgctgccc
     2281 agtagtcccg gaaggcggca agttcagcag gtgtaaattc tgtctccggc aaagccatcc
     2341 cccacaacgc agcccgtcgt cgaaaatccc gtgacggata ccagctatcg ctcatcggga
     2401 attttccgat gggttcgctc aggccataca ggaatacagg gggggctgcc tgtaacgaca
     2461 aaacttcctg ctcactggtc ggagcactct cgcgtgcgtt atgtgtgggg tttagatctt
     2521 tgggttcctt tgggttccgt gatccgtttt tgggtgtctt tgatggaaaa tttgggtgtc
     2581 tttggttatt ttccatgcag ctaagagttc tgtttttggg tctgttttgt gctgaaacat
     2641 aaccattttc ggtactgttt ttattaacag caccaatttt acccaccttt aaagactccc
     2701 gtttttgggt gtattcaggc tcggcaacac tttcttctac accgataagt cggtacacca
     2761 caatttgctt tgttctgcct tttctctcac cggtatcaac aattaaccca atctccatca
     2821 ggtgtcgtaa gctgtcctgc acagtctttt tgttcagttc cgttacttct gccagtgcag
     2881 atacagacgg gtatgcacac aaatcggcac cgcacatatc agcaagccag gtcaatactg
     2941 acttactgga tgaactgccg gttttcacct ttttagccca tcgtagtgca tcgatactca
     3001 tacgaacccc tggcagacat ttgtttatct gcaaagtaat attggtattg ctgacgatac
     3061 gcgtgcttga aagcaatagc tttttctata agctcgtcag tctcacgttc cacaacaact
     3121 ggatccgcaa aaagcagccc ggactccacc acatcgccat attctttgtt taacccggcg
     3181 atcatgtacg taatgctttt tccgtcagta atttcacaat acaatctgaa atcgctgatc
     3241 cggatagcct ccataattgc cggaatcagc gccgtgaatt tgtcacgctt atctctggtg
     3301 tcgatagctt tccagcgttc gaatatcttc acccggttaa cgcccagcgc ccgttgatca
     3361 acctcgccat cattaaacgt gacgcgttga acatcgatgt tcgggcgttc tttcagagcc
     3421 cagaatgctt ccgtgattaa tatcgtcgcc tgctcctgtg tcattcctgg tcggcatacc
     3481 caggtatcca gagcctcaca aacctgttca gtggtgattt tcattgttca accgccccgc
     3541 ccgctttgcc ttacgatatt cgtcataaac tttggggtcg tactgaagtt ccccgccgga
     3601 tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt tccccccatg cagcaacgct
     3661 tgccagccta actcctgcga cattggcaag ctttgttttg ctgccaaaaa acgctatagc
     3721 atcaattttc aacatatcga accccttaga ttttcctaag gaaactagat cgtagagaaa
     3781 cctaagtcaa gaaaaattag aattacctaa tatgaaaaac gaaaccttcg gtgctcgcct
     3841 cttatacagg cgaaaaaaat taaaactgtc tcaggccgca ttaggtaagc tggtcaaagt
     3901 ggctcacgta acaatttctc aatgggaaag agatgaaaca cagccagcgg ggaagagatt
     3961 attcgcactg agccaggcgc ttcagtgctc gccgacttgg cttctttttg gagatgaaga
     4021 taagcaacca ggcgaaccga tcccagataa tcagccagtc aatctgacag aagatcaaaa
     4081 agagttgctt caactgttcg acgcactgcc tgagtcagag caaaaggctc tgttgtcaga
     4141 gatgcgtgct cgagttgaga atttcaacaa actttttgaa gaactactca aagctcgcaa
     4201 aagaagcgca aataaataac cccccttttt ttcgcaactc tctgtaataa aaagcacaaa
     4261 ctttcaaata cttgtgtttt ttacatcaag aagcttaggt ttttctacac aaaaagcttg
     4321 accataactc ttaggctatt ctaaattcta ctcatcaaga caccgcacgg tgttctcagc
     4381 aaacagttcc gctacccggc gttaagggga agcagaggat ttctcagtgg gcgaagtcaa
     4441 acatcagaat ggaaggcgtc cagggatcag caaagaaaca gcgatggcgc tttatattga
     4501 tatcagcgcc attgccggac aggtaagagt tatcagagcg gtaactaagc ggtatgcgcc
     4561 tttacttcag aaagtctctg gtgagtgcac cgaagatatt gtcaacgatt tcgtcatcga
     4621 actgcgagga ctcatcttca gttacaaggt gaccacaatt tttgcagatg gctcccgcga
     4681 aactgtcaga gccctgcggc ttaaaggatg tgtcaaagac ttagccacca cattctgggc
     4741 aagaaaactt gattgtattc ataaccaatt tcctctcgag taacagaccc ctcagaggat
     4801 accacctcgc ctgacgtggt taaaaagcag gcaacgctaa ccacaaggag ccgacatgca
     4861 gaaacgagac cccgtcatca tcgcgccaga ctataccgat gatgaacttt atgagtggat
     4921 gcaccagaaa attaaggcgg tgcaggacct gaaatgggcc aatgaagcca gggctaagca
     4981 ggctgaaaat ctgtccgctc tggagcagga tatcaccaat ctggaaaaag cagcggcatt
     5041 aagcattgcc agaatgatta catacccacg ttaatagcta accaacgagg ctaataatgg
     5101 aatttaaaga tttaccaacg ccattacagg aaatggcatc gaatatagtt cgttcacaac
     5161 tggctactct tgaccttagt accgcagaaa aagaaaccat cgataatatg gttcgtaatg
     5221 tgcgcaatgc tttttctggg ctatatggtt ctgataatca aaagcaggaa agcgatgtta
     5281 ataaacgggt aatttctgtt tgcgtgaatg gccatgttct ttcatcaatc aaaacagaaa
     5341 cggcgacagt cttcgattgc ctttgcattg tacagagcct tgttgatgcc ctgtttcgtt
     5401 cagtgaattt agaaaatgat gcaaatctgc gagggcgcat aatagcacat ccatatgcac
     5461 atactttagg ctctgtggat atcaaagatc ccacaaatct ttaatgaaat agttaacgcg
     5521 aattgtactt gctctttcgg ttgctttcag aatacgcgtt gaaactgctg gcggtaattt
     5581 ggtattccat ttattaaaat catgcccggg aaagtactct tcgaaaatac ttttaactgc
     5641 agactcgcct attgaaatgc tgcttaccat gcgattttga taaaggcatt tagcaataag
     5701 agttgatttt aacattcacc ctcctgaggg ttggtaatta aggagttctc cacgggtga
//
LOCUS       DAZFDP010000238         5753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFDP010000238 DAZFDP010000000
VERSION     DAZFDP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..532
                     /gene="hpaR"
                     /locus_tag="ACHJ1G_004853"
     CDS             86..532
                     /gene="hpaR"
                     /locus_tag="ACHJ1G_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO4766556.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            651..2315
                     /gene="tsr"
                     /locus_tag="ACHJ1G_004854"
     CDS             651..2315
                     /gene="tsr"
                     /locus_tag="ACHJ1G_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4766557.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2358..3629)
                     /locus_tag="ACHJ1G_004855"
     CDS             complement(2358..3629)
                     /locus_tag="ACHJ1G_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO4766558.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3626..4099)
                     /locus_tag="ACHJ1G_004856"
     CDS             complement(3626..4099)
                     /locus_tag="ACHJ1G_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO4766559.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4163..5134)
                     /locus_tag="ACHJ1G_004857"
     CDS             complement(4163..5134)
                     /locus_tag="ACHJ1G_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO4766560.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1535 a         1335 c         1453 g         1430 t
ORIGIN      
        1 agaattgcag gggcagcgca gtcccggtaa cgtgatgatt aactgtttct atattaatga
       61 ttttgaattt ttcaggggaa agataatgca cgactctcta accattgcgt tgctccaggc
      121 gcgagaagcg gcgatgagtt attttcgccc aatcgttaaa cggcataatt tgactgagca
      181 acagtggcgc atcgtgcgta ttctggcgga aagcccatca atggattttc acgatctggc
      241 gtatcgcgcc tgcattttgc gcccaagcct gaccgggatc ctcacgcgga tggaacgcga
      301 cggtttagtg ttgcgattaa agccgatcaa tgatcagcgg aagctctata tctcgctgac
      361 caaagagggg caggcgctgt ataaccgcgc tcagacacag attgaagaag cctatcggca
      421 gattgaagct cagtttactg ccgagaaaat gcaacagtta acccatctgt tagaggaatt
      481 tattgctctg ggtaattccc gacaagaaga catcccgggc gataatgaat aactttttag
      541 tatctgataa ccagcgcctg gtttttccat ggatggcggg ttacaccttt tcataaagtt
      601 tttgctttcc aggccgaaaa tcttgcatcg gtccacagga aagagaaacc atgttaaaac
      661 gtatcaaaat tgtgaccagc ttactgctgg ttttggccgt ttttggcctt ttacaactga
      721 catcaggcgg tctgttcttt aatgccttaa agaatgacaa agaaaatttc actgttttac
      781 aaaccattcg ccagcagcaa tccacgctga atggcagctg ggtcgcgttg ttgcagacgc
      841 gtaacaccct caaccgcgcg ggtatccgct acatgatgga tcagaataat attggtagcg
      901 gttcaaccgt tgctgagctg atgcagagtg ccagtatttc gctgaaacag gcggaaaaaa
      961 actgggcgga ttacgaagcg ttgccgcgtg acccgcgtca gagcaccgcc gcagcggcag
     1021 agatcaaacg taattacgat atttatcaca atgcgctggc ggagctgatc caactattag
     1081 gtgcaggcaa aatcaacgag ttctttgatc agccgaccca gggatatcag gacggtttcg
     1141 agaagcagta tgtggcttac atggagcaaa acgatcggct ctatgatatc gccgtcagcg
     1201 ataacaatgc ctcctacagc caggcgatgt ggattctggt gggcgtgatg atcgtcgtac
     1261 tggcggtcat cttcgccgtc tggttcggta ttaaagcctc gctggtagcg ccaatgaatc
     1321 gcctgattga cagcattcgt catattgcag gcggcgatct ggtgaaaccg attgaggtgg
     1381 atggctctaa tgagatgggg caactggcag agagtttgcg ccatatgcag ggagagctga
     1441 tgcgtaccgt cggtgatgtg cgcaacgggg ccaatgccat ctatagcggt gccagcgaaa
     1501 tcgctaccgg caataacgat ctctcttcgc gcaccgagca acaggccgct tcgctggaag
     1561 agacggcagc cagcatggag caactgaccg caacggtgaa acagaacgcc gagaatgcgc
     1621 gccaggccag ccatctggca ttaagtgctt ctgaaacggc gcaacgcggc ggcaaagtgg
     1681 tagataacgt ggtgcagact atgcgcgata tctccaccag ttcgcagaaa atcgccgata
     1741 ttatcagcgt aattgacggc attgccttcc agaccaatat tctggctttg aacgcggcgg
     1801 ttgaggctgc gcgtgcgggt gagcaaggac gcggttttgc ggtggtcgcg ggagaagtgc
     1861 gtaatctggc ccagcgtagc gctcaggcgg ctcgtgaaat taaaagcctg attgaagact
     1921 cggtgggaaa agtggatgtt ggctctacgc tggtcgaaag cgccggggaa acaatggcgg
     1981 agattgtcag tgctgtgacc cgcgtgacgg acattatggg cgaaattgct tctgcttctg
     2041 atgagcagag ccgtggtatc gatcaggttg gcttagcggt tgctgagatg gaccgggtaa
     2101 ctcaacagaa cgccgcgctg gtggaagaat ctgccgccgc cgccgccgcg ctggaagagc
     2161 aggccagtcg cctgaccgaa gctgtggcag tgttccggat tcagcaacag cagcaacagc
     2221 agcgtgaaac atcggctgtg gtaaaaaccg tgacgccagc tgcgccgcgt aaaatggccg
     2281 tggcagatag cggggagaac tgggaaacgt tttaacgcaa tgacgccgga tgagtatttc
     2341 atccggcgtt tttattatta aaccaggaac agtgaaatcg ccgggatata tgtcaccagc
     2401 gttagcatta atagtagagc cgccagtaga ggcagggatt gtttaaagaa atttcccacc
     2461 gaaacttgcg tgatgttaca taccagcagc atcacggtac ctaccggtgg cgtgaatgcg
     2521 ccaatcgaca ggttgaagat catgatgatg ccaaaatgca ccggatcgat gccaaagtgt
     2581 tcgaccaccg gttttaatag tggtaccagg atcagcaaaa tggcgtttcc ttcaagaaac
     2641 atccccagcg tcagcagcaa tatgttgata attaacagga acatatattt attttcggtc
     2701 agcgaagtca caaaattcgc cactgcgaca ccagcatgtt ccattgagaa aatccatccc
     2761 aacgcggcgc tggccatgat taccagcatc accgatgcgg ttgcgcgtgc cgtttcgccc
     2821 aatgctttga cgacatgaga aatccgcatt tcacgataga taaaaaagcc aatcgccagc
     2881 acgattagca ccgctgctgc acctgcttct gttggggtga aaatgttagc gcgaatacca
     2941 cccaccacag caaaaatgag gaacagcgca ggccaggcct gtcccagcga gaacaccacc
     3001 tctttgcccg taggccagct ttcacgcgag ggtttagcgt tagttttgcg ggcgaccagg
     3061 tagacggtaa acatcagaac gactgcgcat agcaaaccag gtaccagccc agcgataaac
     3121 atttttccga tagagacgtc ggcaaccaga ccgtaaataa tcagggctat tcccggcggt
     3181 atgatcgggg tgactaacga gccaccagcg gtaatggcgg cggcaaacgc gcggttgtag
     3241 cctttacgct ccatctccgg caccatcatg cgcgacagca tcgccgcgtc agcaaggtta
     3301 gaggcgctga cgccgcccat catggtgctg acgagaatgt ttgccacgcc aagtccgccg
     3361 cgcattttcc ccaccagaac gttggcaatg tgcaggatgc gttcggcaat gccggtgtag
     3421 ctcattagtg tgccgagcaa gataaaaaac ggaattgcca gcaatgatgg gctttgcgtt
     3481 ggcgcgatca gtctttgcac ggcaatttca atcggtacgc tggagaagaa taaaaagtaa
     3541 gcgatgatgc caataaacat cgccagatag acccgaatgt tgagaaacaa acagacgatc
     3601 attagcggaa taatcaataa ccaactcatt gcattttgtc tccgctatca gattgtttta
     3661 tatcgtgtat taaccagtag agggtataaa tagccgttgc cagtgcaccg acagggaccg
     3721 ccagatcgat ccagaaccag gaaaccttca gaagttgtgt cactttaaac tgcgccattt
     3781 cggctagtcg gtagcccagc caggccatat acagcagcag tgcgatggag agaacggcga
     3841 ccagtactgc aataacacgc ctgagcttgc ctggaagcat ctccatcagg aagggaatgg
     3901 tcaggtgcgc cctgtcacgt tccgccacca ctccacctaa catcaccacc caaatcatca
     3961 gcattcccga catttcttct gtccattgaa gcggttggcc gatgaaataa cgcataaata
     4021 ccgctccaat tgtcatcaca accagaacga atagcgcaaa ggcagcacta aattctgcca
     4081 ctttgccaaa aagacgcatg aaacctcctg ttagtgcgtg tcctgcctaa gagagatgag
     4141 gcaggacgct gagcattaat gcttattgca gttgctgttg aatggtttcg tacagacctg
     4201 gtgtccattc cgggaattga gagtaaactt cgcgtgcttt cttctggaat ggtccagtat
     4261 cgggatagat cacttcgacg cccgctgcct gcatggtttt caacatttcg gcatcacgtt
     4321 caagagtgag tttctggcta tagacacccg cttcatagcc ggtctggtgg atcatctcca
     4381 gttgttctgg tgaaagggtg ctaaagaagg cttcacctcc tatccatact gaggtatttg
     4441 tcaggtagtt aaccattgtc agatatcttg cctgctcata cagtttttgc ccttgcagca
     4501 ctgaaattgg gttttctacg ccgtcaataa cgccctgggt taatgcggga taaacttcgc
     4561 ctaatggcat gggagttggt gttgccccca tcgcctgaat ggcttttatt tgcatgacgt
     4621 tattaggaac acgaattttt aagcctgcga gatcgtcaac agtgcgaatg ggttttttcg
     4681 aaataatctg tcgagtgcca tagagataat tattcattac cacatgaatt ccctttttct
     4741 tcagactttc atttttttgt ttaaaccagt cgctttcata tattttgaac agtttttgcg
     4801 gatcgtcagt cagatacggt ccaaacaaaa tacccaaatc gggttcataa tcggcaagaa
     4861 aggccacatc actcaaggtt atgacattca tgcccatcat ggcttgctcg gtgacatcct
     4921 gttttgagcc caattgggaa cttggataga gcgcaagtgt tatttcgcca ttactttttt
     4981 tatttaatag gtctgcccag taacgcatga ccacatccag gggttctcca gggttatttt
     5041 cataggcaac ttttatcgaa ataggttttg cctggattat tgacggtaaa aaaagggtac
     5101 tgacagtaat tagcatcgct attattttgc tcatctttac ttactccatg ctaaggaaat
     5161 aaaactccaa cattgttgtc gggagtgaaa ccttatgaaa aagcagtcat tactgaaaag
     5221 tagtcagcga atagcggaat ggctgcaaat gtgtcataaa ttcgtgcacc gggtaatact
     5281 ttgctgcttt taatcggttt ttaatgcatt ttttgattgt atatcttaaa gttatgtaat
     5341 gtatgacgtt acaaggaaca cccactgatg agtttagtta ccgctgataa gatctcttat
     5401 tcggttgatg atttattaaa ataaaagcat taatttggct gcggctattt ttacagataa
     5461 aaactaagtg ttgacgcaga atggtggtat ccattaaatt tatttagtgc tgatttaata
     5521 catttgctga acgtataatt catgctaacg ttaaaagaaa gccatcattc actaaataac
     5581 ttgttcggca aggagatcaa agagtaacgg atgattcgtt acaacggtta aaggtagtaa
     5641 atattcacat ggtgaatttg ttatcaactg gaaaactcat ctcactggtt tcctcctgag
     5701 catgtgagtg aacgctgtga ttaatgatta acgttaagtg ctaaccaggg cgc
//
LOCUS       DAZFDP010000239         5750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFDP010000239 DAZFDP010000000
VERSION     DAZFDP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..1470
                     /gene="mltD"
                     /locus_tag="ACHJ1G_004858"
     CDS             112..1470
                     /gene="mltD"
                     /locus_tag="ACHJ1G_004858"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4766561.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1518..2288)
                     /locus_tag="ACHJ1G_004859"
     CDS             complement(1518..2288)
                     /locus_tag="ACHJ1G_004859"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4766562.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2366..3166)
                     /locus_tag="ACHJ1G_004860"
     CDS             complement(2366..3166)
                     /locus_tag="ACHJ1G_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4766563.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3407..4321
                     /gene="yafC"
                     /locus_tag="ACHJ1G_004861"
     CDS             3407..4321
                     /gene="yafC"
                     /locus_tag="ACHJ1G_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4766564.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4318..5121)
                     /gene="dkgB"
                     /locus_tag="ACHJ1G_004862"
     CDS             complement(4318..5121)
                     /gene="dkgB"
                     /locus_tag="ACHJ1G_004862"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4766565.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5284..5360)
                     /locus_tag="ACHJ1G_004863"
     tRNA            complement(5284..5360)
                     /locus_tag="ACHJ1G_004863"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5324..5326),aa:Asp,seq:gtc)
     gene            complement(5554..5669)
                     /gene="rrf"
                     /locus_tag="ACHJ1G_004864"
     rRNA            complement(5554..5669)
                     /gene="rrf"
                     /locus_tag="ACHJ1G_004864"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1487 a         1400 c         1418 g         1445 t
ORIGIN      
        1 gcgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc ttgtcatcgg
       61 ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca catgaaggca
      121 aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagtaccgg caacgttcaa
      181 cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt tacaagtcag
      241 gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg ggctttcatt
      301 ggcgacgagc taaagatggg aattccggaa aatgaccgga ttcgcgaaca gaaacagaaa
      361 tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc gtatatgtac
      421 tggatagcag ggcaagttaa aaaacgtaac atgcctatgg aactggtact actacccata
      481 gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgccgcagg catctggcag
      541 atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta tgacgcgcgt
      601 cgcgatgttg ttgcttcaac aactgccgcg ctgaatatga tgcagcgtct gaacaagatg
      661 tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg tcgagtcatg
      721 aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc gttaccgttg
      781 ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat tctcaaaaac
      841 agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct ggcgcgtgtg
      901 cacctgagca gcccggttga aatggcgaag gttgcagata tggcggggat ttccgtcagc
      961 aagctgaaga cattcaacgc tggcgtgaaa ggctccacgc tgggcgcaag tggtccgcag
     1021 tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg aatctctggc ttcaggcgaa
     1081 attgctgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag ccgtgtttac
     1141 accgtacgct ctggcgacac gctttcaagt atcgcttcac gtctcggcgt aagcaccaaa
     1201 gatttgcagc agtggaacaa actgcgcgga tctaaactga agccaggcca aagtttgacg
     1261 attggcgcag gcagtagcgc acagcgactg gcaaacaaca gcgatagcat tacgtatcgt
     1321 gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa catcaaagat
     1381 gtgatgcgct ggaacagcga cactgcgaat ctgcaaccag gcgataagct gacgttgttt
     1441 gtgaaaaaca acaacatgcc agattcctga caaaccagat aataaaaagg caccgattcc
     1501 cccggtgcct tttttattta tgccgcttta tgtgcttcta ccatgatgat atcactggtg
     1561 aaagagccgt cattctgcaa ggcaaaatac gttctcacct ctgtcgatgc gctctgttgg
     1621 taaatgcgaa tagcgtctat taacgcttct ggcgtacgca ttctcacgac ccatgaagaa
     1681 aattccagcg gtaacttatc tgtaattaaa ttatcaacta tcagattggc ttcattgatt
     1741 aacgtcaacc actcaccgct ggcgtagttt cgtacgtgag aggtatcgcg taatgcttct
     1801 accgtctgta accagatgtc gcgcactggg tgacccggag acattacgtc catcacaatc
     1861 agcctaccgc caggtttcaa tatccggttc acttcacgca gtgctgcacc aacatcatgc
     1921 cagtgatggg cagaataacg gctgataaca atatcaaatg cgttgtcggc aaatggcaga
     1981 ctttcggcat aaccctggcg ggtggtgata tttttcagtt gccggacttc ggcagcttgt
     2041 gccacgacat ctagcatgtg ggcagataag tcatacgcca ccaccgtgct cacgttttgc
     2101 gcagcgacaa agctggcatg ccctgctccg cagcccatat caagtacact tgcaccagga
     2161 taatcagcca gacgcaccgc caggcgctgt aaatctcgtc cggatgcatg tacggtactg
     2221 gtcagatatt cgctggcttg agagctaaac tgcttttcta catggtcatg gtgggattgt
     2281 attgtcattg cgctgtcctt ttgttatttg agtataaaag ggcagcgcca aacgttggtc
     2341 tgccctgtgg cagacctgac ataccttatt tatcaggctt gccgggactg aattcaacga
     2401 gtagcggatt gtgatcggaa gcgcgcgtaa ccagtacaga agcttcgctg acgttcagac
     2461 cacggtagaa aacaaaatcg agcgggcgac caaacgcccg acggcgctga tcatcggtaa
     2521 aacgcacctg gcgcagcgac atttcccgcg caaagcgata taacgcgttc atccttctac
     2581 ggctccaggc attgaaatct cccgccataa tgaccgggcc gctgtggtga gctatctgat
     2641 caccaatagg aagtaactgc ttactataga catccacgcc cagactgaag ttgacggcgt
     2701 gtatattaac caccatcaac aggcgggtgt caggtaatgg atagaccgtc accagtgccg
     2761 acttcgccag acgcaaaatg ggttctcgtt cacgtaacgg gcagcaatac actggatgcg
     2821 ccgccgaaag ggtcattaca ccggaaggat gttgtggcag cacgaaagcg ggcacctgat
     2881 cggcggcaag atagttagcg gtcgcaaact gtactaactc tggcgttgtc tgcgcttcct
     2941 gcaataacac cagatgtgca tctttgccgt agttctttaa taccgacaac cattcagcgc
     3001 gttgctgttt gtatatgttc cacaccagga tccgaatacg ctcttcagta cttaacggtt
     3061 ctccaggtgg taatgcctgg ccgatgctcg caaaagaccc cggcggtaag atcctttccg
     3121 caggttgtcc ggcaacatag cgcatggcat aggtgttttt tcgcactttt gactttgaaa
     3181 cctctattac gtcaaccctt ccgcactcgg gagggttcta ctgttatagg gattttagct
     3241 cactctttca acgagcaatt gtatattgtt atgtaagcaa gtgcttacac aggtcagaat
     3301 tttgctcatt attgagaaca ttaaatcgga tgacatatca cgcatcctga tggtttaatg
     3361 acaaataaat tcaaaatatc tcttctattt tgcaacagga gcaaaaatga aagccacgtc
     3421 ggaagaactc gccatttttg tttcggtcgt agaaagcggc agctttagcc gggcagcgga
     3481 acaattaggg caagcaaact cagcggtaag ccgggcagtg aaaaagctgg agatgaaact
     3541 tggcgttagc ctgcttaatc ggaccacgcg acaacttagc ctgacggaag aaggccagcg
     3601 ttattttcgt cgcgtacagt caattttgca ggagatggca gcggcagaat cagaaattat
     3661 ggagacgcgt aatacaccgc gtggactgtt acgcatcgat gccgcaactc cagtggtgct
     3721 gcactttctg atgccgttaa ttaagccttt ccgtgaacgc tatccggaag ttactttatc
     3781 gctagtctcc tccgaaacga ttattaattt gatcgaaaga aaagtggatg tcgcgatacg
     3841 cgctggtacg ttaacggatt ccagcttacg tgccaggccg ttatttaaca gttatcgaaa
     3901 aattatcgcc tcccccgatt atatttcccg ctacgggaag ccagaaacga tcgacgattt
     3961 aaagcaacat gtttgcctgg gattcactga acccgcttcc ctcaatacct ggccgatagc
     4021 ctgtagtgat ggacaattac atgaggtgaa gtacggtttg tcatccaata gtggggaaac
     4081 actgaaacag ctttgcctga gtggcaacgg gattgcgtgt ttgtccgact acatgatcga
     4141 caaagaaatc gttcgcggag aattggtgga gttaatggca gataaagtgt tgccagtgga
     4201 aatgccattc agtgcagtct attacagcga ccgtgcggta agtacgcgca tccgggcttt
     4261 tatcgatttc cttagcgagc atgtaaaaac agctcccgga ggagctgtca gagaggctta
     4321 atcccattca ggagccagac cttccgggct aaccaggcgg tcgttgcaat ccagtgcggc
     4381 gatcgctttt ttatcttcgg catcaagctg taaattttgt gccttaagat tactttccag
     4441 gttttcacgt ttagtagaag aaggaattac tgagtaacct tcccccatag cccacgccag
     4501 aatcacttgt gccggagtcg cattgtgttt agctgcgata cgagcaataa cctcatcttt
     4561 caaggctttc ccataagcca gcgtcatata ggaagtaata tggatgccgt gctgtttagc
     4621 ccaggcaacc actttacggt tttgcagata aggagagagt tcaatctggt tagtagcgat
     4681 gttttcagcg ccaataacag caatcgcctt ttccatcaat gggatcgtga agttggaaat
     4741 accgatctca cgcgtcagcc cttgtttttt ggcttccagc agcgcctgca taaactcttc
     4801 aacagagact tcatcgtttg gtgacggcca gtggattagc gtcagatcaa cataatcggt
     4861 acgcaatttt tgcaggctct ctttcagact tgggatcaat ttgtctttgc tgagattttc
     4921 aatccagatt ttagtggtga tgtagagttc atgacgtggc acgccacttt ctgcaatcgc
     4981 ctgacctact gcggcttcgt tatcatagat ttgtgcggta tcaattgcgc gataaccaag
     5041 ttcaagcgcc gttttcacag atgaaataac aacgtcgtct ttcagacgga aagtacctaa
     5101 accaaatgca gggatagcca taatatgcct cttttaatgc tattttcgtt aactgaggtg
     5161 attatgggct tcagactgtt gaggaaaaag agcgtaaaat gcagaggatt tttgcgattc
     5221 tggcaataat agatatccag acgaaaaaaa acctcgagcg ttaactcgag gcttcttaat
     5281 aagtggcgga acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta
     5341 accgactgaa ctaccgctcc accgaattct tttacaacca ccggttttat gaccggctta
     5401 ctgcttaatt tgatgcctgg cagtttatga atcacttcgt gattcaccct tcgggccgtt
     5461 gcttcgcaac gttcaaaagc tttcgctttt gtcctactct cttatcgagt agtgaactgc
     5521 ctcaaagatc agtgccattc ttcgtaattt gatgcctggc agttccctac tctcgcatgg
     5581 ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
     5641 ggtgggacca ccgcgctaag gccgccaggc aaattctgtt ttatcagacc gcttctgcgt
     5701 tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
//
LOCUS       DAZFDP010000240         5731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFDP010000240 DAZFDP010000000
VERSION     DAZFDP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..315)
                     /locus_tag="ACHJ1G_004865"
     CDS             complement(<1..315)
                     /locus_tag="ACHJ1G_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO4766566.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDI"
     gene            complement(572..1207)
                     /gene="msrQ"
                     /locus_tag="ACHJ1G_004866"
     CDS             complement(572..1207)
                     /gene="msrQ"
                     /locus_tag="ACHJ1G_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4766567.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(1208..2212)
                     /gene="msrP"
                     /locus_tag="ACHJ1G_004867"
     CDS             complement(1208..2212)
                     /gene="msrP"
                     /locus_tag="ACHJ1G_004867"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4766568.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(2321..2734)
                     /gene="hiuH"
                     /locus_tag="ACHJ1G_004868"
     CDS             complement(2321..2734)
                     /gene="hiuH"
                     /locus_tag="ACHJ1G_004868"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4766569.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2867..3538
                     /gene="hprR"
                     /locus_tag="ACHJ1G_004869"
     CDS             2867..3538
                     /gene="hprR"
                     /locus_tag="ACHJ1G_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4766570.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3538..4896
                     /gene="hprS"
                     /locus_tag="ACHJ1G_004870"
     CDS             3538..4896
                     /gene="hprS"
                     /locus_tag="ACHJ1G_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4766571.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(5004..>5731)
                     /gene="hchA"
                     /locus_tag="ACHJ1G_004871"
     CDS             complement(5004..>5731)
                     /gene="hchA"
                     /locus_tag="ACHJ1G_004871"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4766572.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT         1594 a         1233 c         1338 g         1566 t
ORIGIN      
        1 gatgtctgtc acataacctt tgcgataata atctttaatt tcagcaaatg ttttggtttt
       61 atctgcatcc gctttcttct gaaagatggg gtcaagtttg ccactttgca gtaaaggata
      121 aacggattgc cagactccat cccagtcact gagcgttcgg ttttgtacat tggcatcatc
      181 aaaaacacca ttagcagctt tttgttcgac ctctgttaag ggtttgccgt gtgagtgatg
      241 accatgcgaa aaggcaggag cgctaacaat aaagacacct aaagcaacag ccagtttatg
      301 aagacgaatc gccaaaacag tttcctccag agttaatgta tatgatgtgt aatgttatat
      361 tgtaacatat aacaaaatgc aacaactctt aggagacaag agaatagttc tctcaggtgt
      421 gcttatctat cttattgaaa taaaataaat tatgtcgatg atttgaggtt gatgacaaac
      481 gaaacataac ttgatgtgat ctgcgtaggc cggatagggc gctcgcgccg catccggcag
      541 ggataacgcg tactttgtcg gcaatatgaa gttatacaga taatttgttg tgaacctgct
      601 tgcgtaaccg gttaaaaagg gaaagcaact tcttataccg taaggctaaa agcagtaccg
      661 ccagcccagc gtagatgagt ggctgcggtg aaataatctt cactgaccac aaataatgta
      721 ttggggccag gatcgcgaca agatagacga agttatgcaa ctgttgccag tgtttaccga
      781 gttttcgctg catcgcctgg gttgaagtga acgctaacgc aagcaaaatt acccagctga
      841 taatacctaa cgttaaataa ggccgggtaa ttaactcctt acctaataac gccagattat
      901 tcacgcccag ctccagcaat gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc
      961 ataagcctaa caggcggcga gtgcgtatca ataacggctg ttttgcgtag cgtgccagag
     1021 gtgtgattaa caaggtcgcc agcaaaaatt tcagtgcagt gcgaccagta aaatgctgaa
     1081 tatctttcac cggatcggca cccaatccac cgtgattaat cgcccagacc agccagagaa
     1141 acggcaacaa tccggcaaga tgcaggcaaa ctttcagcca tgtcacctgt tttgctgtca
     1201 gacgcattta gaaattctcc cgcaaatcca ggccacgata cagcgatgcc acctgctcgg
     1261 cgtaaccatt aaacagtagc gttggctggc gctgtacatc gaggatgccg cctgaaccaa
     1321 taaatcgttc ggtagcctgt gaccagcgcg ggtgatcaac atgcggatta acgttggcgt
     1381 aaaaaccgta ttcgtcaggc gctgccagat tccaggtggt tggcggacgc tcgcgggtca
     1441 gcttaatact gactatcgat ttaatccctt taaagccata tttccacggc acaatcagtc
     1501 gcaccggcgc gccattttgt ggcggtaacg ccttgccata aacacccacg gtcatcagtg
     1561 tgagcggatg cattgcttcg tcgagacgca atccttcgac ataaggatat ttcagcccgc
     1621 caccgataaa gcggtcctgc tggcctggca tttgttccgg tgcataaatt gtttcgaaag
     1681 cgacatactt cgcattgctg gtgggttcgg caagcgccag caatttgtgc agcggaaaac
     1741 caatccacgg caccaccatc gaccatgctt ccacgcagcg catacgataa atacgctctt
     1801 ccagcggaaa gcgacgggtt aaatcatcgt gatcgagggt caatggtttt gccacttcgc
     1861 cgctgatttt cagtgtccat ggatcggttt tcaggctacc agcattagcg gcgggatcgg
     1921 ctttatccag cccgaattca tagaagttgt tataaccgga gactttatct gctggcgtca
     1981 gtggcaggtc attttgccag gcggcaggct tgctgaactc caacggtttt ccggcggggg
     2041 cgggtgggcg atcattccct ttgaaccagc taagcagatc ggcatgcgca gcgtgaggca
     2101 aagaaagtgc agccgcgctg atgcccagtg cttttaacac ctgccgacgc gtcataaaga
     2161 ataccgactc ggccgtaaca tctgattctt ttaaaaattg attctttttc ataagccatc
     2221 acactttttt gtctttagcc agcaagagcc gtttactgtt aatctgtcag gaagtgtcct
     2281 ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca cgataggttg
     2341 aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact ttattaatat
     2401 gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg aaatagtccc
     2461 cggttttaaa tacgacacgg taatcgcccg tagttgcagt ttgctcgggc cacagtgcct
     2521 taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg ttgtccgctt
     2581 tcttttcaag agtgactgtc acgtcggcag cgggttttcc tgtttgctgg ttcaaaatgt
     2641 gcacactaag aatgttttgt tgtgcagcat agaccaaaga aggtaatgaa aatgcagccg
     2701 ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca tgtgaatgag
     2761 ttttgaggat agcgaaaaag cgtccacggt aaccttacag cagcattaca attttgtaat
     2821 gaaccaggct gtttctataa catatgattt atggcatatt attttcatga agattttact
     2881 tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg aagcgggtta
     2941 tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctta aggatgatta
     3001 tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga tcttacaaac
     3061 gttaagaaca gcaaagcaaa ctcctgttat ttgccttact gcaagagatt ctgtcgatga
     3121 cagagtcaga gggctggaca gtggggcaaa tgattatctg gtaaaacctt tttcattttc
     3181 tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctttga attcaacatt
     3241 agaaatcagt ggcttaagaa tggactctgt tagtcaaagt gtgagcaggg acaatatcag
     3301 tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca gagctggcga
     3361 gattatcccc agaacggtta ttgcgagtga aatttgggga atcaactttg atagtgatac
     3421 caatacggtg gacgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga
     3481 aaagctaatc gccacaatcc gggggatggg ctattcattc gtagcggtaa aaaaataatg
     3541 aaaagattat ctataaccgt ccgtttaacc ttgcttttta tattgctgct atctgttgct
     3601 ggcgctggaa ttgtatggac tctctataat ggcctggcaa gtgagttgaa atggcgcgat
     3661 gatacaacac tcattaaccg gacagcgcag atcaagcagt tgttaattga tggggtaaat
     3721 ccagatacgt tacctgtgta ctttaaccgg atgatggatg ttagtcagga tatcttgatc
     3781 attcatggtg atagcatcaa caaaattgtt aaccggacga atgtcagtga tgacatgtta
     3841 aataacatac ctgctactga gacaatcagc gcagctggca tttacagaag cattattaat
     3901 gatacagaga tagatgcttt acgaattaat attgatgaag tttcgccatc attaacggtt
     3961 actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa aattaatagc
     4021 attataattt gcattgtcgc cattgtactt tgctcagtat taagtccgct gttaatcaga
     4081 acgggattac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat
     4141 agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct tgggcaggcg
     4201 ttgaataaaa tgcatcatgc tttagtcaaa gattttgagc gtctaagtca gtttgctgac
     4261 gatctcgctc atgaacttag aacgcccatt aatgcattac tgggtcagaa tcaggttacg
     4321 ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa cattgaagag
     4381 ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgcccgggc agataaaaac
     4441 aatgttttgg tgaaactgga ctcacttttt ctcaataagg aagtcgaaaa tttgttggat
     4501 tatcttgaat acctttcaga cgagaaagag atttgcttta aggtcgagtg caatcagcaa
     4561 atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt taatgccatt
     4621 agatattcac cagaaaaatc gcgcattcat ataaccagtt ttcttgatac caacggctat
     4681 cttaatattg atatcgccag tcctggaacg aaaattaatg agcctgaaaa actcttccgt
     4741 agattttggc ggggagataa ttcgcgtcat tccgaaggtc agggacttgg tctttcttta
     4801 gtcaaagcga ttgccgaatt gcatggggga agtgctacgt atcactattt taataagcat
     4861 aatgtgtttc gaattacgtt accgcaaaga aattaacata gggcttcagt acgccataaa
     4921 tcaaccatga aaaaacgcag ccacgtcgtg agtattaacg tggctgcgat tgattatgcg
     4981 cttacattca tacgtaacag ggattaaccc gcataagctg ccagcatttc ctgcgccgcc
     5041 agtttaccca acgcattcgc tgcgaaagga gtgtcgccgg tgagaacttt acggtcctta
     5101 tgtactcgcc cggtgatgtc gtcattaata atgttcatgc ccattttctt cagctcttcg
     5161 ccgaagtacc aggtgagatg acccggcata tagcctatct ctggcgtttg tttgtctgcg
     5221 gcgtctggga atgcgcaaat ggaataacca ttcagtgggt tatcgccgtg gcgaagcgcc
     5281 agaaaagccg ccgggccgtg gcaaagggag ataacaaaac ggtcattttt gattgcccac
     5341 tgcaaagcag cagccacgtc ctggctttct ggtaaaccaa taagtgcgcc atgaccacca
     5401 ggaacaaaga ttgctgcata ttcgctgtct gcgttgaggc tgacgacaac atccgcgatt
     5461 tttttcggat tgcggaacaa cgatttatgc tgctcaaaga atggcattac tttttcatct
     5521 ttgtgcggca tagcccagta ttcaaacttg gtcatcagac cggaaatggt cgccacttcg
     5581 aattcgaaac ctgcagcatg gagatgatac aacggcagca acgtttcaat cggatggtta
     5641 ccggtcgaga acagttttcc gttatcggtc ggcaaataac gttcgtccgc ggcgatcacc
     5701 agaattttat gtttaccgcg atacggtttt g
//
LOCUS       DAZFDP010000241         5686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFDP010000241 DAZFDP010000000
VERSION     DAZFDP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..372)
                     /locus_tag="ACHJ1G_004872"
     CDS             complement(40..372)
                     /locus_tag="ACHJ1G_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4766573.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(575..880)
                     /gene="ydfV"
                     /locus_tag="ACHJ1G_004873"
     CDS             complement(575..880)
                     /gene="ydfV"
                     /locus_tag="ACHJ1G_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO4766574.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            905..1144
                     /gene="relB"
                     /locus_tag="ACHJ1G_004874"
     CDS             905..1144
                     /gene="relB"
                     /locus_tag="ACHJ1G_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO4766575.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            1144..1431
                     /gene="relE"
                     /locus_tag="ACHJ1G_004875"
     CDS             1144..1431
                     /gene="relE"
                     /locus_tag="ACHJ1G_004875"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO4766576.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            1503..1658
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004876"
     CDS             1503..1658
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4766577.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1875..2126
                     /gene="rem"
                     /locus_tag="ACHJ1G_004877"
     CDS             1875..2126
                     /gene="rem"
                     /locus_tag="ACHJ1G_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO4766578.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            2193..2471
                     /locus_tag="ACHJ1G_004878"
     CDS             2193..2471
                     /locus_tag="ACHJ1G_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766579.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            2473..3522
                     /locus_tag="ACHJ1G_004879"
     CDS             2473..3522
                     /locus_tag="ACHJ1G_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766580.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            3536..4289
                     /locus_tag="ACHJ1G_004880"
                     /pseudo
     CDS             3536..4289
                     /locus_tag="ACHJ1G_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(4567..4656)
                     /locus_tag="ACHJ1G_004881"
     CDS             complement(4567..4656)
                     /locus_tag="ACHJ1G_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766581.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(4711..4923)
                     /gene="cspF"
                     /locus_tag="ACHJ1G_004882"
     CDS             complement(4711..4923)
                     /gene="cspF"
                     /locus_tag="ACHJ1G_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO4766582.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            5224..5439
                     /gene="cspB"
                     /locus_tag="ACHJ1G_004883"
     CDS             5224..5439
                     /gene="cspB"
                     /locus_tag="ACHJ1G_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO4766583.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(5429..>5686)
                     /locus_tag="ACHJ1G_004884"
     CDS             complement(5429..>5686)
                     /locus_tag="ACHJ1G_004884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766584.1"
                     /translation="YALCFAMPAVSFCCETIHAANLHFTTMVSILFLIKILPNMLAIV
                     SCIGPPYFLLKRSNIHYSVRISTQASAFFVIVYRRAMNFNQ"
BASE COUNT         1523 a         1202 c         1423 g         1538 t
ORIGIN      
        1 cgaaaagtgg cccgatgagg gccacaatac ggctgtcact tagacgtaaa tatcaatggt
       61 gccagcggta tttgtatcgt cttttttctc ttctttttta tcaggctgaa ctgtcgcgtc
      121 tttattcttt ttctctgcct gctgccttaa caactgctcc agttgagccc tgaggctttc
      181 aatttgcttc tgtaccaatg cagccatttc ttttttctgc tgtgtcgtca tcccctcttc
      241 cgatgagatt ttcccaagct tttcagtcag cacctgaatt tgtcttgtga ttttggctat
      301 ttctgatgtt ccttccgggg cggagttgtt tgaaataacg gttgaggtat ttccctgaat
      361 tgtgacagac atagatttct ccttttaaaa aagcactatc ggcatgcaca aaaaaatctt
      421 taatcgtatt tcttgtgtca ttaattgttt gatgttcaga ttgttttcct cgcgggctgg
      481 cgcgcctcag aaagtaaagc ttgttgacag gggtaaacgt tcggcaataa ttttctgccg
      541 catgcgggtg ttgcataaaa cgtgctacgt tcctttatcg acaggtcagg tcaccgctca
      601 cccgccgacg agaaagcaac actgacatgc taaagcaaaa aatagatgaa taagttgagt
      661 tgtgcatatg tagcctgacc gtcacaaagt atatggtgtc tgtaccagta agatgatggc
      721 cggactcttt aaaaacgagc tgacctgcac aatacaggat ggacttagca atggctgctc
      781 ctggcacaaa gcggacagtg atcaccgttc ttacgactac tttctgactt ccttcgtgac
      841 ttgccctaag catgttgtag tgcgatactt gtaatgacat ttgtaattac aagaggtgta
      901 agacatgggt agcattaacc tgcgtattga cgatgaactt aaagcgcgtt cttacgccgc
      961 gcttgaaaaa atgggtgtaa ctccttctga agcgcttcgt ctcatgctcg agtatatcgc
     1021 tgacaatgaa cgcttgccgt tcaaacagac actcctgagt gatgaagatg ctgaacttgt
     1081 ggagatagtg aaagaacggc ttcgtaatcc taagccagta cgtgtgacgc tggatgaact
     1141 ctgatggcgt attttctgga ttttgacgag cgggcactaa aggaatggcg aaagctgggc
     1201 tcgacggtac gtgaacagtt gaaaaagaag ctggttgaag tacttgagtc accccggatt
     1261 gaagcaaaca agctccgtgg tatgcctgat tgttacaaga ttaagctccg gtcttcaggc
     1321 tatcgccttg tataccaggt tatagacgag aaagttgtcg ttttcgtgat ttctgttggg
     1381 aaaagagaac gctcggaagt atatagcgag gcggtcaaac gcattctctg aaccaaagca
     1441 tgacatctct gtttcgcacc gaaggtgaca cttctgcttt gcgttgacag gagaagcagg
     1501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     1561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     1621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gacccggcgg gggagaaatc
     1681 cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc cgcttcggcg
     1741 ggtttttgtt tttattttca acgcgtttga agttctggac ggtgccggaa tagaatcaaa
     1801 aatacttaag tagcgcgcag ggataagagg gatggtccct taaaggggag agctaattat
     1861 ccggaaggat tctgatgatg aacatcgaag aactgcgtaa aattttttgt gaagatggcc
     1921 tctatgctgt gtgcgttgaa aatggaaatc ttgttagtca ttaccgcatt atgtgtttgc
     1981 gaaagaatgg ggctgcgtta attaattttg tggatgctcg ggtcacggac ggatttatct
     2041 tgcgcgaagg tgagtttgtc acttcattac aggcattgaa agagatcgga ataaaagctg
     2101 gcttttctgc tttttcagga gaataaactc atctacaatc ttgcgcgggg ctgaactccc
     2161 gctgagtaac accgtgccac cggagaaaac cgatggcacg caacgcaaaa tattacaatt
     2221 ctgataattc gcccgttctt gcctgcacgc acgggcggta ttctcacgca ttcaagtctg
     2281 aatggttcca gcaccctcca tgcactgcag aacaggccga atggctgatt cattcttacc
     2341 gcaggcgcgg gttcgaggtt aagaaagctc tcagtctcga ctatcggcac tggataatct
     2401 ctgtcaggct gccttattcc gaacgcccac cacgtgcgtc ccgcactttc cagcaacgga
     2461 tctggaggta acgtgcgggt attacttaga cctgttctgg tgcctgagct tgggctggtg
     2521 gtccttaagc cgggccgtga atccatacag atatttcata atcctcgagt gctggtggaa
     2581 ccggaaccaa aaagcatgcg taatctgcca tccggagtcg ttcctgccgt tcgccagccg
     2641 ctggcggaag acaaaacatt gctgccgttt tttagtaacg aacgggtgat tcgtgctgct
     2701 ggcggcgttg gcgcattgtc cgactggcta ttacgtcatg ttacatcctg ccagtggcct
     2761 aatggcgatt accatcacac tgaaacagtc attcaccgtt atggtaccgg cgcaatggtg
     2821 ttgtgctggc actgcgacaa ccaactgcgt gaccagacat cggaatcact ggagctgctt
     2881 gctcaacaaa atctgacagc atgggtgatt gacgtcatcc gtcacgcaat aagcggtacg
     2941 caggagcggg aattatcttt ggctgaatta tcctggtggg cggtctgcaa tcaggtggtg
     3001 gatgcactac ctgaggctgt atcgcgtcgt tcgctgggat taccagcgga aaaaatctgc
     3061 tcggtgtacc gcgaaagcga catcgtaccg ggagagcaga ccgccaccag catattgaaa
     3121 caacgcacaa aaaatcttgc accgttgcct tacgcccacc agcaacaaaa atcaccacag
     3181 gaaaagacgg tggtaagcat caccgttgat ccagagtctc cggaatcttt catgaagctg
     3241 cctaaacgtc gccgctgggt taaggagaaa tacacacgtt gggttaagac acagccgtgt
     3301 gcttgctgcg gtatgccagc cgacgatccg catcatctga ttggtcacgg gcagggcgga
     3361 atgggaacaa aagcacatga tctctttgtg ttgcctttgt gcagaaagca tcacaacgag
     3421 ctgcatacgg atacagtggc atttgaagat aagtatggct cccaactgga gctgatattt
     3481 cgttttatcg atcgcgcgct ggcaattggc gtactggcgt aagtggagaa cgagcatgaa
     3541 ccttgaagcc ttaccaaaat attactcccc aaaatctcca aaattgagcg atgacgctcc
     3601 agcgacaggc accggttgtt taacaattac ggatgtaatg gcagcgcagg ggatggtgca
     3661 gtcgaaagca ccacttgggt tggccttatt tctggcaaaa gttggtgttc aggaccctca
     3721 gtttgcgatt gaaggcctgc taaattacgc gatggcactg gataacccga cattgaacaa
     3781 attgagtgaa gaaatccggt tacagattat tccttacctc gtgaattttg cctttgctga
     3841 ttactccagg tctgcggcaa gtaaggctcg ctgtgagcat tgttcaggta cgggatttta
     3901 taatgtattg cgcgaagtgg tgaaacacta ccgacgcggg gaatctgtaa tcaaggaaga
     3961 atgggtgaag gaactatgtc agcattgcca tggtaagggt gaagtcagca cagcgtgcag
     4021 agggtgtaag ggtaaaggga ttgttctgga tgaaaaaaga acccggtttc atggcgtacc
     4081 ggtatataag atttgtgggc gttgtaatgg aaaccggttt agtcgtttac cgaccacgct
     4141 ggcacgacgt catgtccaga agctggtacc agacctgacc gattatcagt ggtataaggg
     4201 gtatgcggac gtcattggta aactgggtaa caaagtgctg gcaggaagaa gcatacgcgg
     4261 aagcgcaatt gaggaaggtg acgagataaa tgatttttgc tgaagatggc gacatgatgt
     4321 ttgcattttt caaaaaatat ggataaaatt ttttcaacga tgggctttgt atacccgacg
     4381 ttaagaaaaa gtagaaaacc cgctgatgcg cgggttttgt gctttaaatg gggcaatggt
     4441 aatgttgaat ctcatcccgg gactcatgtc tgttaactta ttatttagct ggtgacttgg
     4501 ttatttgcct gatgtttaaa atgttttctt ccagtacaat gtccctaaac acaatgagtc
     4561 tgcttattat attattagca gagctattac ggccaaagta cagcataagc ttttaaagcc
     4621 aatcaaccag tcatcaagac agacggggtt attcataaaa actctccatg tgtgatccga
     4681 tggggcctga aattaaagct ttaatatagc tcatgaaagg taaacattgg cagctgaagg
     4741 gccacgcaga ccatttatcc ggcaaaattc cacgcgtaat ccggtggtaa tttcttctgc
     4801 atcgcggaga ttgagcgctg aaacatgaag ctggacatcg atacgaccat cggatggggt
     4861 gataagaccc ttgccgcttt tgccgtcaaa ggttttgaca attcctgtca ttttacggga
     4921 caaaaaaatt ccttaatact gataacttgg cgcactatac acacgttcct gaagaaagct
     4981 atagtttttt gatggggttg aagatggctg gatgtctaaa ataaacattg cttcatatgt
     5041 tcaactatgc gttaatgatt gcgtcggttt gaagaacaga cgatatacga agtagtttac
     5101 taaagcagtt ctcatttcag gtgttattca cttattcctt ctttgagtct ctccaattaa
     5161 gtacgaagtc gtttctgtta tgcaaaccat ttatgccgaa aggctcaagt taaggaatgt
     5221 agaatgtcaa ataaaatgac tggtttagta aaatggttta acgctgataa aggtttcggc
     5281 tttatttctc ctgttgatgg tagtaaagat gtgtttgtgc atttttctgc gattcagaat
     5341 gataattatc gaaccttatt tgaaggtcaa aaggttacct tctctataga gagaggtgct
     5401 aaaggtcctg cagcagcaaa tgtcatcatt actgattaaa attcatcgct cgtctgtata
     5461 cgataacgaa gaaggctgat gcctgagtag agatacggac agagtagtga atattggatc
     5521 tctttaataa aaagtaagga ggtccaatac atgaaacaat ggctagcata tttggcaaaa
     5581 tcttaatcag gaaaagtatg ctaaccattg tggtgaagtg caggtttgct gcatgaatag
     5641 tttcacagca gaagctaact gctggcatcg caaaacaaag tgcgta
//
LOCUS       DAZFDP010000242         5658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFDP010000242 DAZFDP010000000
VERSION     DAZFDP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..338)
                     /locus_tag="ACHJ1G_004885"
     CDS             complement(138..338)
                     /locus_tag="ACHJ1G_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766585.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
     gene            complement(340..1068)
                     /locus_tag="ACHJ1G_004886"
     CDS             complement(340..1068)
                     /locus_tag="ACHJ1G_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4766586.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            complement(1977..2636)
                     /locus_tag="ACHJ1G_004887"
     CDS             complement(1977..2636)
                     /locus_tag="ACHJ1G_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO4766587.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            complement(2629..4236)
                     /locus_tag="ACHJ1G_004888"
     CDS             complement(2629..4236)
                     /locus_tag="ACHJ1G_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4766588.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            4478..>5658
                     /locus_tag="ACHJ1G_004889"
     CDS             4478..>5658
                     /locus_tag="ACHJ1G_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4766589.1"
                     /translation="MSIKSQAYLFKRKDGIWMFQIFVPKYLRHLYPYKLWRKSTSTRD
                     LNEARRFRNHLLLEWEELKRKHNPDNPERRLQQAIESLHSEARANKNTQRAVKRGYAS
                     PSLCLIRDEYAECYRERRSYSTLQKSARAVELFLQSIQKDDIHIEKIGRRLVTVFLDD
                     QKQRGAADQSRQNWLTCLGSLYEFAKRRYDGIPDSNPFHGHNLEARRTIESYQPFTPE
                     QMQKLLDAAEPEIRNIILMGLFSGCRLDELASLKKSEVVTVEGIRCFYISKSKTKAGI
                     RHVPIHSRLSIIVDEYLNHNYGEYLFPHSNKVKRIDGKKGPFYSQAFSRLRRSVLPIA
                     TNRQCFHSLRGMFITCLDRAGVPEQRIGAITGHTEQRAKTEAFRTYSQGASIPELAEY
                     V"
BASE COUNT         1483 a         1337 c         1388 g         1450 t
ORIGIN      
        1 tagacatagt aattactcct cttgagagtt gagactatcg gtatcgcaga cacagtaatg
       61 cctacgggtc tccccgcatc ggtcatctca aaaggtttta aataaggttg gttaaggtct
      121 ggaaatttcc ggagatgtta agactccagt ttcgcaaagg ctcgacgtaa ctgattattc
      181 aaatcacggt cgaccagcat cgctatcagc tcgtctcgac tgtagtaagg gcgatcaggg
      241 ttatcgactc caagtctcag cagggacagg aggtgacggg taggcagacc acggtagcgg
      301 agagttgcac gaccaatagg tgtgcgcggg tcaatcatct tagagtccct ccagcttgcc
      361 ggattgggct tgcttcagga gatgctcttg cttctgctgg tcggtgccag tgaaggcaat
      421 aaggatgcct cgcagtttat cgactatcgc aggcttaccg aagacctcca atcccttagc
      481 gttgaagcga aagttataac cacctgctga gaacttctgg tctgacggct taatgatacg
      541 gaagcagtcg gaatactttg gatttacaga ccactcgtta ctgattgggt tcttacgaaa
      601 gagaccacga tgctgaagaa gtgcggtcat tagcttctga gcgggtagac cataggtttt
      661 gcagtagtcg ccccaagtca cgcctcggcg gttagcggac tgaatggctg ctcgcttctt
      721 ctggtcttcc agttgaagga ctcgacgctc taacgtctga atatggtcgt aacacttggc
      781 gcggaggtga acggaatagc cagcgaccag aatgaaagta tgcttacggt cgagacggta
      841 cagaggctgc tgtttcccct gcgcgtttag atagacatct tcagtcaccc cgatatgact
      901 gctcagtttt gagcactgct cagttttgag cgcgttaagc tcctcagcca tattgcgaat
      961 atcacgtagg acatttcggt gctccttgcc tgtcagctca gcaatttcaa ctgaggtcat
     1021 ggagacaagc tggtcgaggt taatgacgct gatttcttgt tgagacattt acgtgtactc
     1081 cttttgtttg caccctgaag tcgcaccccc actgacctac tcaagccccc acgtaaaggg
     1141 agaccagcag gcaacaggac gcaaacagaa tgacttgagg taattggggt tagtggtctg
     1201 cgatgaccat agagatgaag gttcgacagc atgtccgagc gtgtgtggtg atagacttaa
     1261 gtcacgagac caaagaacac aaaggctgac cagaggctgg tggctagcaa gctagcgtgg
     1321 accacggttt ctcgcttgcc gtctctcagg aggtcagagg cgagatatgg tccctatagt
     1381 gatagtttag tcaaacagga atgaacttcg gagaacctga cgcgaaagtg ttcagtactt
     1441 ccagttagac ccgaagtatg gagaaccaca tcatagcgac cgtagtatac ttcgggctga
     1501 caggactgga ggctggactt aaagttcttc ttattggaat tgtgataaca caatccataa
     1561 caagatgaac tcatggtctc ctcaagcctg ctggtcaggc tcaaagctcc aacctcagac
     1621 accactggtc taaccttttt gagtttccgc ctgtccacaa atggatggca gacctcagca
     1681 atgatgtctt ggttcttcac aatctcgcac accgatggtc gcatagacgg atacttccgc
     1741 tgcggtctga tgttctccgc ctgtcaaagg agagagattg ctacgttaaa gggaatggtc
     1801 tcaggccaag gtgatttaac actaggctta ttgcggatgg tttccaatcg gtccgctacc
     1861 ggaagcctga gacttaatga atgtgtgggt cggtggctgt attaagcccg gggggactta
     1921 gggtctctcc cctatagtga tagtttagtc cccccccttc ccctatagtg tgggtttcat
     1981 tcgttagcgg cctgctgctg ctccctcgcc tgtctgtcct gattctcaat accttgacgt
     2041 ggaccttcag gcatactctg gaacatagcc tcaatttctt tcggtctcgt gattcggtca
     2101 tcgacaataa tgttcagcag cttaaagaga ccgtgtgcca actgcgggtc atcatcaaga
     2161 ctaatctctc cgggatggac cgcctgattg ccgacaatac gacagatatc tgccgcctgc
     2221 tggatgcgta caggtagtcc tttctggacg agtgaccgga tgtctttatt aatgttttct
     2281 cccggctcgc ctaagtggac cattagcttc tgaaggcaaa gacgaaggag tgcagcggcc
     2341 cctttaggag acagattaat gatgcttcgg gcttccatat agtcggactt gcagttatct
     2401 ggcatatctg gatttggtag ttcaacattg ccagccgccg gaataatcat attgccgtca
     2461 taccaataag catcatcacc gcaatgagag catgttgaca ttcggaactc ggttgtcctt
     2521 tgcgccaact cacgcgcact tccatacatc ctacgccaga actgacgggc atatacacca
     2581 cagtgaggac agttaaaagc ctctgcttca aacaccgccg gataatactc agccatttaa
     2641 tccagtctcc ttataaacac ctgccatcac attaaccaat ctatccacct cgacctcttt
     2701 agcgtaacgg tcgtaagtta tagaccctga gctatgaccc ataatcgcct gagcatacgc
     2761 taactcgacc ccatgttgtt ttaactgggt cgcaacagtg tgcctgaatg agtgaaaggc
     2821 taagccgcta cggtcagcaa ggactttagg cagcaacgtt ctgttaaacc atttgctcac
     2881 ctgctcaccg tagccattct tcatcagctt gaggccatcg aataccatag cattatcacc
     2941 cgcagtcttt ctacggtctt ccacaagcga cataaagtca gccaatacaa aaccataagc
     3001 gccatcgacc agcgggacac agcggtcgct atgagcgttc ttaatacttt tacccggtag
     3061 gctgctatcg tcttcgttga tatggatata tacggtcccg gcctcagtag ccctgacgtc
     3121 tttgacctga agctgggcca cctcgttaag tctcgcacct gtaatcgcag ccagagcggt
     3181 gacgtaataa tggtatggct tacctgcggt cttctgagag tacgctttag cggcgaccag
     3241 tagttgccct acctgctcag gactgaaagc atcacgagcg tcggaggctt tacgctgcgg
     3301 gactttcagc tcaagacctt cggtcaggtt cttagctatc aggtcgttac gtaccgccca
     3361 cttaaagaca gcagccatct tgataagata tttattgttg atggtaacga catcaagaca
     3421 gtcagtctta gactcccggt tcagcaggtc agacagcggc atagacttaa agcgttgctt
     3481 gcggttcttc ggtagctgtt ggaggacttc acggacccta agcatatctg cacgggtcgc
     3541 cttgccgaca tcctgaatat ccagatagtc gaatatctcg attagcgtag agtgtgacgc
     3601 tcggttttcg cttagggtcg ctggcttcca gttctgggcg ttctcctgct cgtactgctc
     3661 aatgagagac gcgagggtca gtgctttagg ttcagggact tcaggctccg atgatacaga
     3721 tagggagacg gacggtctta gactgccagt ctcaggctca aggtaagaca acaggccaga
     3781 gttatcgccc ttgagtctat cctgacaggc tttaagtaca ccgataacgt cattgatata
     3841 gcgatgctgg tctacagtca gacgctcagt cgctacagct tcccgaaggt tggacttgat
     3901 gtcgtcgtac tggtcaacgt aaagctggta ggactccggg tcgttcaggt catcacggct
     3961 gacaccaagt tcccactcaa caatggtctt caggtgctcc cgtagttcct catatgatgc
     4021 gctagggttg tctgcgtgta ttgctttaag tgctgacttg atgtgccgtg agtgagccat
     4081 agcggtacgt ctgtccttag tccgtagtga tactgacgct agtttatacc ggttgtgaga
     4141 aggaagcgaa gcggtcaggc gaccagtggt cttcatcctg agacggatgt aaaagacacc
     4201 gttacgctga taaaggtagg ttcttgaatt gagcatgtac tgagacctta ggtctgctca
     4261 ctacttcaac aaagttagtc acagtgttgg ttacagtgag taaaactatc ggaaggcttt
     4321 aagccgtatc ccttaggatt cagtaagtta gtagacttta agaatggtca tggagcgggc
     4381 gaagggaatc gaggaaaccc ataataacct gctgtaaaat aaagattttg tgatcaatca
     4441 taggtaactg attacgtgat aatagaggat cattatcgtg tcgatcaaga gccaagcata
     4501 cttatttaag agaaaagatg ggatctggat gtttcagatt tttgttccta aatacttacg
     4561 tcatctttac ccttacaagt tgtggagaaa aagcacatct acacgagatc tgaatgaggc
     4621 gagaagattc agaaaccatc tattattaga atgggaggaa ctaaagcgga aacataatcc
     4681 tgataacccc gaaaggaggt tacaacaagc tattgaatcc ttacattcag aggcaagggc
     4741 caataaaaac acgcaaagag ccgtaaaacg cggttatgca tcgccttccc tatgcttaat
     4801 acgtgacgaa tacgccgaat gctacagaga gcgtcgttca tactcaacac tacagaaatc
     4861 agcaagggcc gttgagctat ttttacaaag cattcagaaa gatgatattc atattgagaa
     4921 aataggcaga cggcttgtaa cagtattctt agatgaccag aagcaacgag gagcagccga
     4981 tcaatctcgt caaaactggc ttacatgctt aggatcgctc tatgaatttg ctaaacgccg
     5041 ttatgacgga ataccagaca gtaacccttt tcacggtcat aatctcgaag cacgtcgcac
     5101 aatcgagagt tatcaaccgt ttacacctga acaaatgcaa aagctacttg atgcagcgga
     5161 gccggagatc agaaatatta ttttaatggg cttgttttca ggatgcagat tagacgaact
     5221 ggcaagtctg aaaaagtcag aggttgtaac tgtggaagga atacgctgtt tttatatcag
     5281 caaaagtaag actaaagcag ggatcaggca cgttccgata cattcgagac tatcgattat
     5341 tgtcgatgaa tatttaaatc ataattatgg tgaatatttg ttccctcact cgaacaaagt
     5401 aaaaagaatc gacggtaaga aaggcccgtt ctattctcaa gcgttctcac gtttacgtcg
     5461 tagcgtcttg ccaatcgcta caaatagaca atgctttcac agcttgcgcg ggatgtttat
     5521 cacttgtctt gatcgtgccg gagtgcctga acagcggatc ggtgccatta ccggacatac
     5581 agagcagaga gctaaaacag aagcctttcg cacctactca caaggggcaa gcattccaga
     5641 attagctgaa tatgtaga
//
LOCUS       DAZFDP010000243         5508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFDP010000243 DAZFDP010000000
VERSION     DAZFDP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(226..834)
                     /locus_tag="ACHJ1G_004890"
     CDS             complement(226..834)
                     /locus_tag="ACHJ1G_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO4766590.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1260..1328)
                     /locus_tag="ACHJ1G_004891"
                     /pseudo
     CDS             complement(1260..1328)
                     /locus_tag="ACHJ1G_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1587..1934
                     /locus_tag="ACHJ1G_004892"
     CDS             1587..1934
                     /locus_tag="ACHJ1G_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766591.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2139..2840)
                     /locus_tag="ACHJ1G_004893"
     CDS             complement(2139..2840)
                     /locus_tag="ACHJ1G_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4766592.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2837..3856)
                     /locus_tag="ACHJ1G_004894"
     CDS             complement(2837..3856)
                     /locus_tag="ACHJ1G_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4766593.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3853..4392)
                     /locus_tag="ACHJ1G_004895"
     CDS             complement(3853..4392)
                     /locus_tag="ACHJ1G_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO4766594.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(4462..4692)
                     /locus_tag="ACHJ1G_004896"
     CDS             complement(4462..4692)
                     /locus_tag="ACHJ1G_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4766595.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4797..5486
                     /locus_tag="ACHJ1G_004897"
     CDS             4797..5486
                     /locus_tag="ACHJ1G_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4766596.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
BASE COUNT         1395 a         1311 c         1255 g         1547 t
ORIGIN      
        1 attggatatc attttcggta tttctctgca aattccagcc cacaggatct cggtgtacaa
       61 tctgtcgctg atcgcgcaaa tttagggtca atcacttcat tttttgcatc aaaccctcac
      121 taacatcgaa cgattatagt acaagtagtg ttcttgactt ctcattaaac cgtcagcatt
      181 caattaatta caaaatacta gcggcatttc tctcccgcaa ctttattaca gccgttcaga
      241 acgtaaatgc gtcaatactc gactccactt atcatcctgc cacggccgga attttacatg
      301 tgccgtttct ctggcaagga ttgcctgtgc cttattgagt atccggggat attcttgttc
      361 gattgaagtg aagcgaccgg cttcgcggtg ttctgctacc tgaagaagcg gagtgacgtt
      421 ttggcaagcg gttaacattg tgtcacttgc ccgccataac caagcaagtg tgcaaagttc
      481 gttatcagtg aattgttttg tgattgggga ttgttgaatt tctcgatcga gaatatccag
      541 cacccagcgg cggaattctt tggccacagg agtgcgggcg aacatggcga tcagatgggc
      601 gccacggagg gagaaaatgc gagtcttctt acgataattt cctgaggtcg ttgattcgac
      661 gacctgagac attccgtcag taaactcatc aatatttttg ttgtagatgt cggttactgc
      721 tttggtgctg gcatatttta atgctgccgc caattctttg ctggtaaacc agatctggtt
      781 gttgtgattt ataggagtga attttacatc gcggaatgcg agctgagtga ccatagcgat
      841 aatctcgttt tgaatttgag ctatcaccac cgacgacgcc aatcgactgg tggtggactg
      901 tgcaaggttg gcgtaaccgg tcaaaacgaa atccggcgct tccgaagaag ccctcacaca
      961 gcccaccata atttttgtgt agacgtgcac tgcgcataaa aaaaccgcga acgcggttat
     1021 gcgtcgtttt gatgtccggg acgccaatcc cgtgtgccga ttttgcggca acgcacagaa
     1081 tatagcgccg gataaataat gtcgtcaacc cttgcatatg gggggcgcga tgatcactac
     1141 taccaccata atcgctccaa ctgttgcaac tactttaggc taagacatca cgatttccat
     1201 atcaccttta gccgccagac aaacagagac tccctgttct tggctgccaa aaacaccgaa
     1261 tgcaaaaaat cgcaggtgta tttctgcatc tgtggaggga gaagagagtt tggattgtat
     1321 gtgcgcataa acgtccccgt ttagcgcaga agtcaccgta gttgttcata ttctgatgac
     1381 atgataattt cctattgaat agaaaaaatt aacacgactc attcttatga ctggaaaaag
     1441 caatcatgct aataatcatt gttgttccag ttccaaatgc agcgaaataa tcaccaatat
     1501 tttttctacc ggtttcgact ttgatgctat attctgctaa cacctaacta acactacgtt
     1561 tcacacagca attacatgga aataacatga ctatcaaagc gactgacata gagattcttc
     1621 atcgttatgc tgaaggggta atggaacgtt ctaaccatca tgcaaaaaat gttggtgcag
     1681 ccgctcttac attgctagga ggtgttatct ggaaagccct gcccggatca atcgagataa
     1741 gaacgtataa cggaagtctt gcaaatatgg tgtggtggca aagcgaaaga acattaaaaa
     1801 attatgcaat ctcatacaac cataattcat gtgaaattga aatgagagat gaatcagtca
     1861 aaggtgctgt attatttagc atttccaacg aaaccactcc agaaaaaata ttatcacagc
     1921 tttctgagct ttaattaaag gcggtgctaa caccgcccat accattactc cacatcttac
     1981 gcaacttgat aattatgctc tattaaatac ccacaatatt tcatcaatgc atgggtcagc
     2041 tacaaaatgt ttaccgtggt aattcctgtt ttcgcaacat ctcaaaagaa caataaacac
     2101 tgtatttctt caagtaacga agaatctttc tttgcccgtc atacacttgc tcctttcagc
     2161 ccaaacttag ctttgatttc tgcgatcttc gccagagcct gtgcacgatt tagaggtcta
     2221 ccgcccataa caggaagttg ttttactggt tcaggtatcg tctcaccacg gttaattcgc
     2281 gctgtcatac aggtcagttc atcggcagcc ttgcgccgta attccgcgtc agtcagcgca
     2341 ttggcccgca tgttctggta caggttggta accaaccagt aatgcgcgtt cgatttccac
     2401 ggataagact ctgcatccgg atacaggcca cgcttccggc aatactcgta aaccatatca
     2461 accagctcgc tgacgtttgg cagcccggcg ttaacggatg cttcttcccg gcaccatgca
     2521 acaaactgcc cgggtgatgg cagaaatggt ctattctgcc gacgggcaac acgcattccg
     2581 gcgctcacct gttccatcgt ggtgatcccg ttttcccgga aagccagaac ccactggcgg
     2641 cggatttcgt tcagttcgtt ctggtcacga ttagccaggc tcgccgggaa agttgccagt
     2701 aactggctga atacaccgtt gattatctgc gctacctgct gtacctgcgg cttttcgtcg
     2761 tactgttccg gcatgttgtt ggcgatccga cgcatctgct cacggtcaaa gttaatcatc
     2821 tgtgcggcga tgtttttcat agatccaccc cgtaaatcca gtctgtgttt gtcaggtcga
     2881 gttttggttt gccgactgtc acgccagcct gttgcttgtt ccggttgatt tcgagctggg
     2941 tccacttgtc gcggagtttg gccggactca gcacgttacc ggaccagaag ttgtcctggc
     3001 aggcccagcg gaacagtaca cacatgtcgc ggtggttacg tccatcacgt tcacgcatca
     3061 gacggatatc gttagcccac cctgcaaaat tcggttttct ggctgatggc gcgatggtct
     3121 tcaccatgtc aaacatccac tctgcggcgg tcaggtcttc tgctgtcccc cacttgctgc
     3181 cgctctgaat cgcagcatcc gctttcacca caggaaggtc gttttctggc aggtcagagg
     3241 attcgccaga attctcggac gaataaggtt ttatattgtc ttttgttagt ttgtcttttg
     3301 tgtttacctg attcgggtaa gtgcctttac ctgatttggg taaacttttc ttacctgatt
     3361 caggtaaatt tacctctttc aggtaaactt tatttttctt acctgattcg ggtaatgttg
     3421 accattcact gaccacatta ttaatgccga tattccgccc gctctgaata aaaatcccac
     3481 gctttaccag aacacttttt gcagcagaac acttgtgcgg caatatcccg gtcaactcgg
     3541 aaagttgctc gttgctcacc cagtccagtt ttttattaaa gccatatgtt ttgcgcatga
     3601 cagccaggaa gaccagaagc tggtgctgtg ttaatccggc cagcattaca gcttccagca
     3661 actcatttgc aatgcgcgta taaccatcat cgagatctgc cacgcgcggc tccttttgtg
     3721 ccgcatccgg cactggaaaa ttgaatatct cagcagtgtt tgccataatt cctcccgcaa
     3781 tgagtgtgtt acgatttgca cctgaaagtc ggttctgttc cagcagaccg gctttcgcca
     3841 tttctgaacc tgtcatatcg cccccagcat ggtagtaacc atcgccatca atggaccagc
     3901 cagctctggg tccacacgaa acatcgacac aataccttca ctaatttcct tcagtttctg
     3961 gtggcgtggt gcgttgagaa tgacagcctg ttttgcctca ctgagttcct tttccatttc
     4021 agccaaccta gccatgaagc tatcctgctc aaccaggtaa ccgcgatatt ccagcggtag
     4081 taccgccaga attgccgggg tcagttcacg cacgttattt cggtattttt cagaatcgaa
     4141 tttgttatcg aggaagcgga acagcttctg gcgtgtacgg ctgacatcat cagggaaatc
     4201 gatggtgccg ccgccctgct cccgatactc attcacaatg agtgtggcaa cgacatcctg
     4261 attatctaca gccgaccagg cgcggacggc atcacggatt ttttcgtggc ctggcacctg
     4321 ttttgtttga gaacgattta tcaccgcagt cgggctaaat ccgctagtct gttggtatgg
     4381 aagtggttgc ataattgact cctttagttt gaattgactg ttaagttgat tgcttattgt
     4441 taaagagcgt gaaatggaaa tttaagctgc gttcttttcg gtgtgtggaa acaacttcgg
     4501 aagatccggg cgaatctggt atgccttcac tactccacca gtagccgtaa caatgctgcc
     4561 gacatgttca ggggatacct ttgctttgtt gtgaagccac ttatagacgg cctgctgtga
     4621 aacttcgcag gcagcgccca gtttcttttg tgaaccaacg atattgatcg ctgttttgat
     4681 agctgggttc ataacaacct ccgtggttaa tttgaatcaa gattaaaact atggttgttt
     4741 ttagtcaaca accattttcg tttgatggaa taaaaccttg gttgtacatt tggactatga
     4801 aaacaacact ctcagaaaga cttaaagaag ccagattagc gcgaggcctt acacaaaagg
     4861 cgcttgggga tttggtcggg gttagccagg ctgctattca gaaaatcgaa acagggaaag
     4921 ctaatcaaac aactaaaatc gtggagatcg cgaacgcttt gggtgtgcgc gcagaatggt
     4981 tatcttctgg cgttggaaat atgtcagaca gtacagtgca accaatacaa tcaactgtca
     5041 gccattccaa atacttcaag attgacgttc ttgatataga agtcagtgct gggccgggag
     5101 tcatcaaccg tgagtttgta gaagttctac gctcggttga gtactcgttt gacgatgctc
     5161 gtcacatgtt cgatggtagg aaggcagaaa atatccgcat cattaacgtg cgtggtgaca
     5221 gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg gttaaatctt
     5281 tcgacggtga tggtatctat gcatttctgt acgacgacac cgcccatgta aagcgcctgc
     5341 aaatgatgaa ggataaactg ctggttatct ctgataacaa gagctactca ccgtgggacc
     5401 ctatcgagaa agacgagatg aaccgggtat tcatattcgg taaggttatt gggagcatgc
     5461 cgcagacgta cagaaaacac ggataatcag ccgcgtgttg atgaggct
//
LOCUS       DAZFDP010000244         5339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFDP010000244 DAZFDP010000000
VERSION     DAZFDP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..552
                     /gene="dfrA8"
                     /locus_tag="ACHJ1G_004898"
     CDS             43..552
                     /gene="dfrA8"
                     /locus_tag="ACHJ1G_004898"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HFO4766597.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            549..1205
                     /locus_tag="ACHJ1G_004899"
     CDS             549..1205
                     /locus_tag="ACHJ1G_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO4766598.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLAGHYHNHKKIGTVANSRW
                     "
     gene            1242..1514
                     /locus_tag="ACHJ1G_004900"
                     /pseudo
     CDS             1242..1514
                     /locus_tag="ACHJ1G_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1547..4534)
                     /locus_tag="ACHJ1G_004901"
     CDS             complement(1547..4534)
                     /locus_tag="ACHJ1G_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO4766599.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMTYFSEQDLAIINAHRK
                     PASRFGFAVLLCYLKSIGFAPDKKSSPPAKLLKTIGARLKLSAELWQEYVSGRDTTRR
                     EHMRELYRYLELKAFNGALQKECIRHLLPLSTRTDRGILIAEELLKYLHQQRIIIPGI
                     DVLERTCAEAMTLGDKTVYTTLNAHLDITHKSALDALLVTNEKQISRLTWLLQPPGKI
                     NGKNVLQHIDRLNAIEALALPEGVERTVHQNRLLKLAREGRKMSSRDLADFSPARRYA
                     TMVCVLTEARATIIDEIIELHERILGSMFSRAKHQQAERLQKTGKLIQQKLRQYVSVG
                     QAILDARNAGEDPLSAIERVLPWDDFVASLEETKLLARKDNFEPLHLITEKFSTLRKY
                     SFRFLSALQLRATSAALPLNDALETIRDMYRKQLRKVPASAPLDFIPESWRKMVVTPS
                     GIDRQYYEICAMNELKGALRAGDIWVRGSRRYKNFDDYLMPNDDFEKLLNNNLLNLPV
                     GTECGAYLNARLTLLASRLEEVNAMAVAGELPDVDISDKGVKITPLDNSVPSTVSPLA
                     DLIYNMLPHPKITEILDEVERWTAFSRHFTHLKNPLIRPQDNRLLLTTILADGINLGL
                     AKMAESCPGTTRASLENIQAWYIRDETYSAALAELVNAQKSRPLAALWGDGSTSSSDG
                     QNFRVGSHGRYAGQVNLKYGQEPGVQFYTHISDQYSPFYTKVISRVRDSTHVLDGLLY
                     HESDLDITEHYTDTAGFTEHVFALMHLLGFAFAPRIRDLHDKRLFIQGKADNYPALQS
                     IISTTPLNIKEIKIHWNEILRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGR
                     IERTLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRGIENQSYRAS
                     GLTLLTAAITLWNTVYIENAINLLKKKGLHINEQLLSHLSPLGWEHINLTGDYIWKNN
                     KKLAEGKYRPLRAVDVSQYKKQP"
     gene            4701..>5339
                     /locus_tag="ACHJ1G_004902"
     CDS             4701..>5339
                     /locus_tag="ACHJ1G_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4766600.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELKRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYKEVKPD"
BASE COUNT         1402 a         1278 c         1282 g         1377 t
ORIGIN      
        1 gacgcaataa tcaccaattc ctcttttgat aggtaaacaa caatgatcga gcttcatgcc
       61 attttagctg ccaccgccaa tggttgcatt gggaaggaca acgcacttcc ctggccacca
      121 ctaaaaggcg atctggccag attcaaaaaa ttgaccatgg ggaaggtggt cattatgggg
      181 cgcaagacct atgagagctt gcccgtcaaa ttagaaggtc gcacctgcat cgttatgacg
      241 cgccaagcgc tggagcttcc gggtgttcgt gacgctaacg gcgctatctt cgtgaacaac
      301 gtcagcgacg ccatgcggtt cgctcaagaa gagagcgtgg gcgatgtggc ctacgtcatt
      361 ggtggcgctg agatattcaa gcgacttgcc ttgatgatca cgcagattga attgaccttt
      421 gttaagcgac tgtacgaagg cgacacctac gttgatctgg ccgaaatggt caaagactac
      481 gagcagaatg gcatggaaga acatgacctt cacacttact tcacttaccg taaaaaggag
      541 cttacagaat gaccttcgtt gccatttcag atacgcacct gcacaactgg tcgcagttcg
      601 cgataccaac agagtcgggc attaactcca ggctgttgca gattcttaaa gccattgaag
      661 aagcggcttg cgccgccgat taccacgccc cagcgggcgt ggttccaacc gtctatcacg
      721 gcggcgatct cttccatgtg cgaggttctt tgaccccttc tgtgctaaac gcggtgctcg
      781 acttttttaa gaccatccac cgtgactacg gcgttcgctt tcgcatgatc gccggcaatc
      841 acgatctgga aaccaaagac tcatgtccta tgggcaatgc agctgcggcg cttaattcgt
      901 tgccgtttgt ggaggttgta tccgaaaaaa cgctgttcga ggaccacaaa gtcgctctgc
      961 tgccatggcg cgattccatg gacgatctgc gtgcggatct tgcgcacgtt aaagacgcga
     1021 tcggcgctag tgttgcctct aagtggacag caatcatcca cgcaccggtt aacggggttg
     1081 ttcttggcat tccagaccat gggttcgacg gaaaagagct cgcctcctat ggcttcgggt
     1141 tagtgctcgc cggtcactac cacaaccaca aaaaaatcgg cactgttgca aatagtcggt
     1201 ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc
     1261 ggcattttca gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca
     1321 gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga
     1381 tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc
     1441 gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc
     1501 gctgggcgta tctgggctcc gtttgggaaa cggaaaatat tgtacgttaa ggctgttttt
     1561 tgtactgact gacatcaact gctcgcaaag gacgatactt cccttcagcc agcttcttat
     1621 tgtttttcca gatatagtcc cctgtcagat taatgtgctc ccagcccagc ggagacaaat
     1681 gtgacaacaa ttgctcatta atatgtagcc ctttcttttt cagcaggtta atggcgtttt
     1741 ctatatatac ggtattccac aaagtgattg cggcagtgag cagcgtaagc ccactggcgc
     1801 ggtaactctg gttttcaata ccacgatccc ttatttctcc cagacgatgc atgaagacag
     1861 ctctggcaag ggcattccgg gcttcccctt tgttcagacc tgcctgaacc ctacgacgaa
     1921 gcgccggatc gcggaaccag tccagcatga acagtgttcg ctcaatacga ccaatttctc
     1981 ttaacgcctt agcaaggcca ttctgtttcg gataactggc caattttttc agcatcagag
     2041 aagcggtcac tgttccctgt tttattgagg tcgccagtct cagaatttca ttccagtgta
     2101 tttttatctc tttgatattc aggggggttg tggagataat tgactgtaga gcaggatagt
     2161 tatcagcttt accttgaata aacaaacgtt tgtcatgcag atcccggatc cttggggcaa
     2221 aagcgaatcc cagcagatgc attagtgcaa aaacatgctc cgtgaaacct gctgtatcgg
     2281 tgtaatgttc cgttatatca aggtcacttt cgtgatacag aagaccatca agtacgtggg
     2341 tggaatcacg aacacgactg attactttgg tataaaacgg actatattga tctgagatat
     2401 gcgtataaaa ctgcacccca ggttcctgac cgtacttcaa attaacctga ccggcatagc
     2461 gcccatgact gccaacccta aaattctggc cgtcagagga tgaggtggaa ccgtctcccc
     2521 ataatgccgc cagcggtcgt gacttctggg cattaaccag ttctgccagt gcagcagagt
     2581 acgtttcatc acggatgtac catgcttgta tgttttccag cgaagctcgt gtggttcccg
     2641 gacaggactc tgccattttt gccaacccta gattgatgcc gtccgccagg atagtggtta
     2701 gtagtaaacg attatcctga ggacggatta gcggattctt aagatgagta aaatgtcgcg
     2761 agaaggcagt ccatcgctcg acttcatcaa gtatctctgt aattttgggg tgcggtagca
     2821 tgttgtaaat caggtcggcc aggggagaga ctgttgaagg aacgctgtta tccagtgggg
     2881 tgatctttac acctttatct gaaatatcca catcaggaag ctcccctgcg acagccattg
     2941 cgttaacttc ctccagacgg gaggccagta gagtcagtct ggcattaaga taagccccgc
     3001 attctgtgcc aacaggtaaa tttaataggt tattatttaa taacttttcg aaatcatcgt
     3061 taggcataag ataatcatca aagtttttgt agcgccggga gcccctgacc cagatatcac
     3121 ccgcccgcag cgcccccttt agctcattca ttgcgcaaat ttcatagtat tgtcggtcaa
     3181 tgcctgaggg ggtgaccacc atctttctcc agctctcagg aatgaaatcc agtggtgccg
     3241 atgcaggaac ctttcgtagc tgtttacggt acatatcacg aatagtttca agggcatcat
     3301 taagcggcaa cgctgccgaa gtggctctca gttgcagggc cgatagaaaa cgaaaagaat
     3361 atttgcgcaa tgtactgaat ttttcggtaa taagatgaag cggctcaaaa ttatccttac
     3421 gtgcaagaag ctttgtttcc tccagactgg cgacaaaatc gtcccacggt aacactcgtt
     3481 caatagcaga cagaggatct tcgcctgcat tgcgtgcatc caggatagcc tggccaacag
     3541 aaacatactg acgtaacttt tgctgtatca acttgcccgt tttctgaaga cgctcggcct
     3601 gctgatgttt tgcccgactg aacatgctgc ctagaatacg ttcatgcagt tctataattt
     3661 catcaatgat tgtggctctg gcctcagtca gtacacagac catcgttgcg tatcgacgag
     3721 caggggaaaa atcagccaga tcgcgactgc tcattttgcg gccctcacgc gcgagtttca
     3781 gcaatcggtt ctggtgaacg gttctctcca ctccttccgg cagcgccaga gcttcaatag
     3841 cgttaaggcg gtcaatgtgt tgcaggacat tctttccatt gattttccct ggaggttgaa
     3901 gcagccacgt caaacgggat atttgttttt catttgtcac caacagggca tcaagcgcag
     3961 atttatgcgt gatatcaagg tgcgcattca gcgttgtata caccgtttta tcacccagtg
     4021 tcattgcttc tgcacaggta cgttccagaa catcaatgcc tggaataata attcgctgtt
     4081 gatgcaggta ctttagtaac tcctctgcga taagtatgcc tctgtccgtt ctggtagaca
     4141 gagggagcag atgccggata cattctttct gaagtgcacc gttaaaggct ttcagttcaa
     4201 gatatcgata aagttccctc atatgctcac gtctggtggt atcacgaccg gaaacgtatt
     4261 cctgccaaag ttctgctgat aacttaagtc gagcgccaat ggtttttagc aattttgcgg
     4321 gtggggatga ttttttatcc ggggcaaacc caatactttt cagataacag agaagaacgg
     4381 caaaaccaaa ccggctggca ggtttacggt gagcattgat aattgccaga tcctgctcac
     4441 tgaaatacgt cattcgagtc agtgttaact catcgtctgg caatgccagt aatgattctc
     4501 tttccgatag agaaagaatc tgcctccttg ccatatagtt atccttgtta attatttagt
     4561 acgttatttt tgatgaaaag gttattgcat aagggtacac cctgatgcga gatataaaat
     4621 gtgacctcat acaaataatg acataaccta tatgcgatag aatatatgca acaacctaaa
     4681 tgcgacaatg cggtgaaaaa atgttcatca gagcttattt aagggcgtca acagaagatc
     4741 agtttgcaga tcgagcaaaa gagatgctgg aacagttcgt ccaagaacgg gggcataaaa
     4801 ttgccagtta ttatcgggag aacataagcg gtacaaaact tgaccgtccg gaactgaaac
     4861 gcttactgat ggacagtcac cgtaatgata ttttgctggt cgagcagata gaccgcctga
     4921 cccgactgag taacagcgac tggataacgc tgaaaaagca gatagaacaa cacgaactgc
     4981 ggattgtcag tctggatgtt ccaacatcat ggcaagccct gtcagataaa gatccttcac
     5041 aatcagatcc gattacccgc gcagtgataa ctgcaataaa taatatgctt atagacctga
     5101 tggccgcgat gtcgcataag gactggctga gtcgtcgcca acgccaaaaa cagggaattg
     5161 aacgagctca tacgttgggt aaataccggg gaaaacaggc cgatcaggaa cgtcatcaga
     5221 aagtgttgta ctacagacaa gtaaaaaaac tcagcattag agaaacagca gatgcaacgg
     5281 gctacagcac ttcgcaggtt tgtcgcattc aggctctcta taaagaggtt aaacctgat
//
LOCUS       DAZFDP010000245         5337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFDP010000245 DAZFDP010000000
VERSION     DAZFDP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..407
                     /locus_tag="ACHJ1G_004903"
     CDS             39..407
                     /locus_tag="ACHJ1G_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO4766601.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            480..644
                     /locus_tag="ACHJ1G_004904"
     CDS             480..644
                     /locus_tag="ACHJ1G_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO4766602.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            613..777
                     /gene="kil"
                     /locus_tag="ACHJ1G_004905"
     CDS             613..777
                     /gene="kil"
                     /locus_tag="ACHJ1G_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4766603.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            832..1128
                     /locus_tag="ACHJ1G_004906"
     CDS             832..1128
                     /locus_tag="ACHJ1G_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4766604.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1134..1919
                     /gene="bet"
                     /locus_tag="ACHJ1G_004907"
     CDS             1134..1919
                     /gene="bet"
                     /locus_tag="ACHJ1G_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4766605.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1916..2593
                     /locus_tag="ACHJ1G_004908"
     CDS             1916..2593
                     /locus_tag="ACHJ1G_004908"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4766606.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2593..2775
                     /locus_tag="ACHJ1G_004909"
     CDS             2593..2775
                     /locus_tag="ACHJ1G_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4766607.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2748..2939
                     /locus_tag="ACHJ1G_004910"
     CDS             2748..2939
                     /locus_tag="ACHJ1G_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4766608.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            3016..3231
                     /locus_tag="ACHJ1G_004911"
     CDS             3016..3231
                     /locus_tag="ACHJ1G_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4766609.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3330..3551
                     /locus_tag="ACHJ1G_004912"
     CDS             3330..3551
                     /locus_tag="ACHJ1G_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4766610.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            3548..3853
                     /locus_tag="ACHJ1G_004913"
                     /pseudo
     CDS             3548..3853
                     /locus_tag="ACHJ1G_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            4150..4500
                     /locus_tag="ACHJ1G_004914"
     CDS             4150..4500
                     /locus_tag="ACHJ1G_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4766611.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            4575..4817
                     /locus_tag="ACHJ1G_004915"
     CDS             4575..4817
                     /locus_tag="ACHJ1G_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766612.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            4936..5280
                     /locus_tag="ACHJ1G_004916"
     CDS             4936..5280
                     /locus_tag="ACHJ1G_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766613.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1546 a         1204 c         1349 g         1238 t
ORIGIN      
        1 aatgtgaggg cgattttttt ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
       61 tcattgatta cgactggaaa acatcaatag aaattgaaat cgaccatgac ttaatgacag
      121 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
      181 acggctctgt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
      241 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
      301 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
      361 catcaggaat atttgatcca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
      421 gcataccaat tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
      481 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
      541 gaattacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
      601 gagtcccgaa aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact
      661 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
      721 atacagaaac tgagatcaag caaaagcatt cactaccccc ctttcctgtt ttcctaatca
      781 gcctggcatt tcgcgggcga tattttcaca gccattttca ggagttcagc catgaacgct
      841 tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca gcagatcgcc
      901 cgtgaagaga aagaggcaga actggcagac gacatggaaa aaggcctgcc ccagcacctg
      961 tttgaatcgc tatgcatcga tcatttgcaa cgccacgggg ccagcaaaaa agccattacc
     1021 cgtgcgtttg atgacgatgt tgagtttcag gaacgcatgg cagaacacat ccggtacatg
     1081 gttgaaacca ttgctcacca ccaggttgat attgattcag aggtataaaa cggatgagta
     1141 cagcactcgc aacgctggct gggaagctgg ctgaacgtgt cggcatggat tctgtcgacc
     1201 cacaggaact gatcaccact cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc
     1261 agttcatcgc attgctgatc gtcgccaacc agtacggcct taatccgtgg acgaaagaaa
     1321 tttacgcctt ccctgataag cagaacggca tcgttccggt ggtgggcgtt gatggctggt
     1381 cccgcatcat caacgaaaac cagcagtttg atggcatgga ctttgagcag gacaatgaat
     1441 cctgcacatg ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt accgagtgga
     1501 tggatgaatg ccgccgcgaa ccattcaaaa cccgcgaagg cagagaaatc acggggccgt
     1561 ggcagtcgca tcccaaacgg atgttacgtc ataaagccat gattcagtgt gcccgtctcg
     1621 ccttcggatt tgctggtatc tatgacaagg atgaagccga gcgcattgtc gaaaataccg
     1681 catacactgc agaacgtcag ccggaacgcg acatcactcc ggttaacgat gaaaccatgc
     1741 aggaaattaa cactctgctg atcgccctgg ataaaacatg ggatgacgac ttattgccgc
     1801 tctgttccca gatatttcgc cgcgacattc gtgcatcgtc agaactgaca caggccgaag
     1861 cagtaaaagc tcttggattc ctgaaacaaa aagccactga acagaaggtg gcagcatgac
     1921 accggacatt atcctgcagc gtactgggat cgacgtgaga gctgtcgaac agggagatga
     1981 tgcgtggcac aaattacggc tcggcgtcat cacagcttca gaaattcaca acgtaatagc
     2041 aaaaccccga tcaggaaaga agtggcctga catgaaaatg tcctacttcc acaccctgct
     2101 ggctgaggtt tgcaccggtg tggctccgga agttaacgct aaggctctgg cctggggaaa
     2161 acagtacgag aacgacgcca gaaccctctt tgagttcact tccggcgtga atgttactga
     2221 atccccgatc atctatcgcg acgaaagtat gcgcaccgcc tgctctcccg atggtttatg
     2281 cagtgacggc aacggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt
     2341 ccggctcggt ggtttcgagg ccataaaatc ggcttacatg gcccaggtgc agtacagcat
     2401 gtgggtgaca cgaaaagatg cctggtactt tgccaactat gacccgcgca tgaagcgtga
     2461 aggcctgcat tatgtcgtga ttgagcggaa tgaaaagtac atggcgagtt ttgacgagat
     2521 ggtgccggag ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatatgg
     2581 ggagcaatgg taatgaagca tcctcacgat aatatccggg taggcacgat cactttcgtc
     2641 tactccgtta caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg
     2701 aaagcacagc ggctggctga agagataaat aataaacgag gggctgtatg cacaaagcat
     2761 ctcctgttga attaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca
     2821 ggtttgtgcc aataccagta gaaacagacg aagaatttca tacgttagcc acatcccttt
     2881 cacaaaagct ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac
     2941 aacaacagaa tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga
     3001 catgggactt gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag
     3061 gctaattctg aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt
     3121 tgccgtagcg tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact
     3181 catttaagtc tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa
     3241 gcaaaatcat cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta
     3301 ttaaccaaca attcagggat taatggaaga tggcagacat cattgattca gcatcagaaa
     3361 ttgaagaatt acagcgcaac acagcaataa aaatgcaccg cctgaaccac caggctatat
     3421 ctgccactca ttgttgtgag tgtggcgatc cgatagatga actaagacgt ctggccgttc
     3481 agggttgtcg gacttgtgca agttgccaag aggatctgga gcttatcagt aaacagagag
     3541 gttcgaagtg agcgaaatta actctcaggc actgcgtgaa gcggcagagc aggcaatgca
     3601 tgacgactgg ggatttgatg cggacctttt ccatgagctg gtaacaccat cgattgtgct
     3661 ggcactgctg gatgaacggg aaagaaacca gcaatacatc aaacgccgcg accaggagaa
     3721 cgaggaaatt gcgctaacgg tagggaagct gcgtgttgag cttgaggaag caaaatcaaa
     3781 actcaacgag cagcgtgagt attacgaagg tgttatctcg gatgggagta aacgtattgc
     3841 tgaactggag aaaagcgaag agcaactcat taacgagcgt gaccatgctg agtctgcttt
     3901 agctgatatg tacttcgcaa caaccgggga taggcctgag tggagtaact gtttcagttt
     3961 ttcagatgct gtcgatgccg tagttgacag aattgctgat ttagaagcca aacagccatc
     4021 gccagtagta ccggaagaaa aaccaatgcc taaccctctt agcatgtaca cagttgatgc
     4081 tgttgcagct attgcagagg tgagaggctg gaacgcctgc cgtgcagcca tgcttaaggg
     4141 agataaatca tgattaatcg aaccaaactg gagcacatcc tcgagtatgc caggcagcag
     4201 aggcgctttg gccagctttg taaaattctg ccaggagata tggttgaaat cgtggagatt
     4261 gccatgcgta agactggcaa ctctccggta agtccgggtg gttggataag ctgtagtgag
     4321 cgaatgcccg ctcaagatga ttggatttta atttattcaa agcacggtga gtatatggca
     4381 ggacaggtac aagaggaata cgtggagttg agcgacggca ctttatcgtg gttagggaac
     4441 gccttgtact ggatgctgct accagaaccg ccgcagggag tgaatgatga attggcctga
     4501 agcattcacc gctgtaggag ttgcaatcgc ggtggcattt attctgtatt cgcttttccg
     4561 ctggggataa aggaatgttc gctctgattc aacgtggtca gatatacacc gatagcgccg
     4621 gctacccgat aaaaattctt cgctgcataa acaacactgt gttgtacaga agaatggatg
     4681 ggcgaacaca gtcggtaaaa ataaacgatt ttaatgaact gtttgaacgg atcgatcacc
     4741 aggaataccg acaaattctg gctgaaacag agcaggagaa ccatctgaaa aaattacgcg
     4801 ccatgcaaag gagataaacc ggtaaaggtg ttcgcgataa aggtgaatat cggcaatgaa
     4861 taacaatcct cgcactcgcg gggatttctt ttatctgaac tcgctacggc gggttttgtt
     4921 ttatggagac aagaaatgtc agatttggct atgaaggttt tgaaatggca atcgactggc
     4981 gatgttggca tcagtagcgc aactcttgcc tcaatcgcat gtggactgaa aaagaatatc
     5041 tatggtcatc acttcggcgc tccacatgac gcagcagact tccggcgatg cgttgcactt
     5101 gttgagcaga ttccagaaat cagagattca ttcgacaagg ttgcaaagcg cgttccggca
     5161 ttcaaaggca tcctcaacga atgggattcc ctcgtcgctc tgttgaagtc tgaaatgaag
     5221 atacacggaa acaaagcacc agagacttac agaagaatta gcgagctacg caaggactaa
     5281 ccacagcctc acactcaatg aggcctgttc atttctcaag atatccagac ctaccat
//
LOCUS       DAZFDP010000246         5206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFDP010000246 DAZFDP010000000
VERSION     DAZFDP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..698)
                     /locus_tag="ACHJ1G_004917"
     CDS             complement(<1..698)
                     /locus_tag="ACHJ1G_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO4766614.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRV"
     gene            complement(813..888)
                     /locus_tag="ACHJ1G_004918"
     tRNA            complement(813..888)
                     /locus_tag="ACHJ1G_004918"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Thr,seq:ggt)
     gene            complement(895..969)
                     /locus_tag="ACHJ1G_004919"
     tRNA            complement(895..969)
                     /locus_tag="ACHJ1G_004919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Gly,seq:tcc)
     gene            complement(1085..1169)
                     /locus_tag="ACHJ1G_004920"
     tRNA            complement(1085..1169)
                     /locus_tag="ACHJ1G_004920"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1133..1135),aa:Tyr,seq:gta)
     gene            complement(1178..1253)
                     /locus_tag="ACHJ1G_004921"
     tRNA            complement(1178..1253)
                     /locus_tag="ACHJ1G_004921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Thr,seq:tgt)
     gene            1615..2565
                     /gene="coaA"
                     /locus_tag="ACHJ1G_004922"
     CDS             1615..2565
                     /gene="coaA"
                     /locus_tag="ACHJ1G_004922"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4766615.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2594..3559)
                     /gene="birA"
                     /locus_tag="ACHJ1G_004923"
     CDS             complement(2594..3559)
                     /gene="birA"
                     /locus_tag="ACHJ1G_004923"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4766616.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3556..4584)
                     /gene="murB"
                     /locus_tag="ACHJ1G_004924"
     CDS             complement(3556..4584)
                     /gene="murB"
                     /locus_tag="ACHJ1G_004924"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4766617.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4887..5002)
                     /gene="rrf"
                     /locus_tag="ACHJ1G_004925"
     rRNA            complement(4887..5002)
                     /gene="rrf"
                     /locus_tag="ACHJ1G_004925"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5097..>5206)
                     /locus_tag="ACHJ1G_004926"
     rRNA            complement(5097..>5206)
                     /locus_tag="ACHJ1G_004926"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1304 a         1344 c         1187 g         1371 t
ORIGIN      
        1 tctacacgac cggtaacaac ggtaccacga ccggagatgg agaatacgtc ttcgatcggc
       61 agcaggaacg gcttgtcaat cgcacgctct ggttccggaa tgtaagaatc caggaagcca
      121 gccagttcca ggattttcgc ttcccactct gcgtcgcctt ccagcgcttt cagagcagaa
      181 ccacgaacga tcggagtgtc gtcgcccggg aagtcatact gagacagaag ttcacgaact
      241 tccatttcaa ccagttccag cagctcttcg tcatcaacca tgtcgcattt gttcaggaac
      301 acgatgatgt acggaacgcc tacctgacga cccagcagga tgtgctcacg agtctgcggc
      361 atcgggccgt cagtcgcagc aactaccaag atcgcgccgt ccatctgagc agcaccggtg
      421 atcatgtttt taacatagtc ggcgtgcccc gggcagtcta cgtgtgcgta gtgacgggtc
      481 ggggtgtcgt attcaacgtg agaagtgttg atggtgatac cacgagcttt ttcttccggc
      541 gcgttatcga tctggtcgaa tgcgcgagca gcaccgccgt aggttttagc cagtacggta
      601 gtgattgcag cggtcagcgt tgttttacca tggtcaacgt ggccgatagt accgacgtta
      661 acgtgcggtt ttgtacgttc aaacttttct ttagacatcg attgtccctc taagacacgg
      721 ataaatcggt gatatcacca catcaaccag gcaacatgcc cgacttgttg aatgcaataa
      781 acagaaggaa aaaacaggga ggagaaaaga agtggtgctg ataggcagat tcgaactgcc
      841 gacctcaccc ttaccaaggg tgcgctctac caactgagct atatcagcac atcttggagc
      901 gggcagcggg aatcgaaccc gcatcatcag cttggaaggc tgaggtaata gccattatac
      961 gatgcccgca tcctggaact cggctacctg attttcattc tgcactgaat atcgagagaa
     1021 gctctcttta ttcgagccgg taagcgaact tatcgtctcg ggctacgcca tcgcgtgccg
     1081 aaattggtgg tgggggaagg attcgaacct tcgaagtctg tgacggcaga tttacagtct
     1141 gctccctttg gccgctcggg aaccccaccg gacttgatgg tgccgactac cggaatcgaa
     1201 ctggtgacct actgattaca agtcagttgc tctacctact gagctaagtc ggcatcaagt
     1261 agcgcgcatt ctatggagac atgcgagttc atgcaactaa aaaattgcat aatttgtttt
     1321 attggtcaca ttttatgcga cacgatgaag aaacagccga acggagggca gattagcaca
     1381 ctttttcaac atcattgtgc tcaacaatgc gctcctgcta aaccataatt ctttttatca
     1441 gatggaatat ctgtcacatt gcttttcaac gatagcttcc tggcagagat tttttcttat
     1501 tattcctccc catctggtgt taccctcctg cccattaacc cattcaacag aactgtgacg
     1561 cgccatggca aatatcgctt tgccgataga gctatgaccg ccagaaacat gcttatgagt
     1621 ataaaagagc aaacgttaat gacgccttac ctacagtttg accgcaacca gtgggcagct
     1681 ctgcgtgatt ccgtacctat gacgttatcg gaagatgaga tcgcccgtct caaaggtatt
     1741 aatgaagatc tctcgttaga agaagttgcc gagatctatt tacctttgtc acgtttgctg
     1801 aacttctata taagctcgaa tctgcgccgt caggcagttc tggaacagtt tcttggtact
     1861 aacgggcaac gcattcctta cattatcagt attgctggca gtgtcgcggt ggggaaaagc
     1921 acaaccgccc gtgtattgca ggcgctatta agccgttggc cggaacatcg tcgtgttgaa
     1981 ctgatcacta cagatggctt ccttcaccct aatcaggttc tgaaagaacg tggtctgatg
     2041 aagaagaaag gcttcccgga atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat
     2101 ctcaaatccg gcgtgccaaa cgttacagcc cctgtttact cgcatcttat ttatgatgtg
     2161 atcccggatg gagataaaac ggttgttcag cctgatattt taattcttga agggttaaat
     2221 gtcttacaga gcgggatgga ttatccacac gatccacatc atgtatttgt ttctgatttt
     2281 gtcgattttt cgatatatgt tgatgcaccg gaagacttac ttcagacgtg gtatatcaac
     2341 cgttttctga aattccgcga aggggctttt accgacccgg attcctattt tcataactac
     2401 gcgaaattaa ctaaagaaga agcgattaag actgccatga cattgtggaa agagatcaac
     2461 tggctgaact taaagcaaaa tattctacct actcgtgagc gcgccagttt aatcctgacg
     2521 aaaagtgcta atcatgcggt agaagaggtc agactacgca aataatttgc aggggagcga
     2581 atactcccct ttcttatttt tctgcactac gcagggatat ttcaccgccc atccagggtt
     2641 ttattattcc atcctgctca agtaataaag ccccctgttt atctattccg cgtgaaatgc
     2701 caaatatttc tttatcacca atgataagtt tcactgggcg attaataaaa ttatccagct
     2761 tttcccagcg cgacagataa ggtgccaatc cttcttgttc gaagagttcc aacgcagcac
     2821 gtaattcacg tattagcatg gccgccaacg tattacgatc gagattgatc cccgcttcct
     2881 gcagcgtgat ccacccctga ttaacgacac tctcttcaac acggcgcatt gccatgttga
     2941 tcccggctcc aatgactatt tgcgccgcat cgccagtttt gccagtcagc tccacaagaa
     3001 tgcctgccag cttgcgatcc tgcagataga ggtcattagg ccatttgaca cgaactttat
     3061 ctgctcccag cttgcgtaat acttccgcca tcacgatacc gataaccaga cttaaaccaa
     3121 tcgccgccgc cgggccttgt tccagacgcc agaacatcga caaatataag tttgcgccaa
     3181 aaggcgaaaa ccatttccgc ccccggcgac cacggccagc ctgctggtat tctgcaacac
     3241 aggcatcgcc cgatttaagc tctccgatac gatcaagaag gtactgattc gtggagtcaa
     3301 taactggcag cacggctaca ctaccgccat ccagctgacc caatatctgt tcagcattaa
     3361 gtaactggat gggctcaggc aggctgtatc ctttacccgg aacggtaaag acatcaacgc
     3421 cccagtcacg cagtgtctga atgtgtttat taatagccgc ccggctcatt cccagcgttt
     3481 cacccaactg ctcgccagag tgaaattcac cgttcgctaa cagggcaatc aatttcagtg
     3541 gcacggtgtt atccttcatg aaattgtctc cactgcgctc acttcacctg atgcaccaat
     3601 aaagcggact tcaggctcaa gccagacatt aaatttttca cccacttttt gtcttacatg
     3661 gtgtgccagt tgcaccacat cttcgctttt tgcattgtct tcattaatga gaactaacgc
     3721 ctgttgacgg tgcaccgcag ccccacccat ttgcatccct tttagctggc actgatcaat
     3781 aagccaacct gctgccagtt ttactgaacc acccgcctgg gggtaatttg gtgctgttgg
     3841 aaattgcgcc agtaatgctt tagccgtttc ggcagataca acagggtttt tgaagaaact
     3901 accggcattg ccattcactt ttggatcagg gagtttggtg gtgcgcatat gacacaccgc
     3961 attaaatact tgctgtggcg ttactgttgt aggatccaga cgagttaagt caccatacgt
     4021 tagtacaggt tgccactctt ttggcagacg cagacctacg gctacaatgg cgaagcgatc
     4081 ctggtattca tgtttaaaaa tactgtcgcg atagccaaaa cggcactctt tggcagttaa
     4141 gcgcacttgc ttgcctgtcg ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa
     4201 ttctacgcca taagcaccaa tattctggat aggtgatgag ccgacacaac caggaattaa
     4261 tgccagattt tccagaccag gcataccttc ctgcaaagtg tattttacca ggcgatgcca
     4321 gttttctccg gctcctacat gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc
     4381 tttgatccgg ttgatgatca ccgtgccgcg atagtcttcc agaaaaagta cattacttcc
     4441 ttcacccaga ataagaacgg gttgtccttc tgcggttgca tactgccagg cattgagtaa
     4501 ttgttgttcg tcttcggcac atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt
     4561 ccagggtttt aaggagtggt tcatagctgc tttcctgatg caaaaacgag gctagtttac
     4621 cgtatctgtg gggggatggc ttgtagatat gacgacagga agagtttgta gaaacgcaaa
     4681 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
     4741 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
     4801 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     4861 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggaaacc
     4921 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
     4981 ccaccgcgct aaggccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
     5041 taatctgtat caggctgaaa atcttctctc atccgccaaa acatcttcgg cgttgtaagg
     5101 ttaagcctca cggttcatta gtaccggtta gctcaacgca tcgctgcgct tacacacccg
     5161 gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc ttaaag
//
LOCUS       DAZFDP010000247         5203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFDP010000247 DAZFDP010000000
VERSION     DAZFDP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..203)
                     /locus_tag="ACHJ1G_004927"
     CDS             complement(<1..203)
                     /locus_tag="ACHJ1G_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4766618.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWT"
     gene            complement(211..606)
                     /locus_tag="ACHJ1G_004928"
     CDS             complement(211..606)
                     /locus_tag="ACHJ1G_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4766619.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(603..1181)
                     /locus_tag="ACHJ1G_004929"
     CDS             complement(603..1181)
                     /locus_tag="ACHJ1G_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766620.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1193..1546)
                     /locus_tag="ACHJ1G_004930"
     CDS             complement(1193..1546)
                     /locus_tag="ACHJ1G_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4766621.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1558..1953)
                     /locus_tag="ACHJ1G_004931"
     CDS             complement(1558..1953)
                     /locus_tag="ACHJ1G_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4766622.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1995..3020)
                     /locus_tag="ACHJ1G_004932"
     CDS             complement(1995..3020)
                     /locus_tag="ACHJ1G_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4766623.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(3076..3408)
                     /locus_tag="ACHJ1G_004933"
     CDS             complement(3076..3408)
                     /locus_tag="ACHJ1G_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4766624.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3418..4737)
                     /locus_tag="ACHJ1G_004934"
     CDS             complement(3418..4737)
                     /locus_tag="ACHJ1G_004934"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4766625.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(4718..>5203)
                     /locus_tag="ACHJ1G_004935"
     CDS             complement(4718..>5203)
                     /locus_tag="ACHJ1G_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766626.1"
                     /translation="LSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWL
                     EEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLST
                     YEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRA
                     A"
BASE COUNT         1057 a         1617 c         1375 g         1154 t
ORIGIN      
        1 gtccagtccg cgtcttcatc atccaggtag ttatcatcgt agggttctgc cgtcatctcg
       61 cccggcgtca gatccttcac cttagccagt cgctgccagt catcgtctga caacgggttt
      121 gcataagcat cacccttgcc gttgtaaacc cacagagtgg taccggcacc ttttaccggc
      181 tcaaggggat ttggtgttgc catatcgtcc tcacatctcg tatgtaatgg aataagtcag
      241 atctgcagaa ctccataacg ccatatcgtc atcacgacga tactcatagc cctgcgtaac
      301 catcgtggta atcagtcctg ccagtgccgg gatatcgctc atcaccggat aaatccggga
      361 ctccatccac gcatccagct ctgaatccgg cacctgagca ggcaggaaaa cttcaatatg
      421 cagctccgcc tgccaggtat cgctgtccag cgcttcgccc gtgtattcag cgccggtgag
      481 ataaacggca actgccggaa aatccgcctc atcaaaaaca gcggggcgac catcaaaaag
      541 cgtcgccccg gtgtcatgct tctccagtgc atccagtacg gctgcacgga gttcagtatg
      601 tttcatcgct ttattaccat tctcagttga tgctgcagcg catagcccag ctctttcgga
      661 agacgttcac gccgtatccg ttcaatattc tgtttaaacg ccgtggtaag cggcaccgcc
      721 atcgggattt tcaccacatc aatggggtaa cggtttttcc cggccacacg ctgcatgaca
      781 tgccaccgcc catttttcag ttgctgaata aacgcgccgg gaatacgacg gtttcccacc
      841 acaagcacgc tgccgccacc tttcagggat gaacgctgcc cctttttacg acgcctgcgt
      901 cgggacagga caacccgcgc attccccagc ttgattacgg gcaaatcccc ccggttaacc
      961 ttgattctgg cctgcggatt tttaaccgtg gcccttttca gcctggccct ttcctttacc
     1021 agtttccggc gtacctttgt ctcacgggca acctgtgacg cagactgcga tatcgcggat
     1081 gaagcaacgc ggttaatggc cattgctgac gcaccgggca ccgccgtttt gctgatacgg
     1141 ctgaggtttt caacggcctg ctcaagacct tttatggcca tacatccccc tttcagcggc
     1201 gacggttaac ggcaggcggc acgccacgcc caagccagag atgacagctt ccgccatcat
     1261 ccggcgaaat ccggtctatc cagaagtttt cctcaccgat ggtcagcgtg tctccacgcc
     1321 gcagctgccg cacatcatca gtccggacaa acagggacgg gctggagcct tcaacgcgca
     1381 cgccctgtcc ggcatagctg atattttcag ggtcatcaaa aacaccacgt attactgcgc
     1441 cggactgctc accggatgtc atggtggctg acgttcccat gtacccgcgt atcgtttcat
     1501 cggcgcgggc aatggcagca tcgaacaggt tatcgaaatc agccacagcg cctcccgtta
     1561 ttgcattctg gccaggccac gttctgtcat ttcggctgcc acaccggcag agacacgaaa
     1621 cgccgttccc ggcagcacaa atgccacagg ttcatcccgc gtggcgtgaa gtgcatcggt
     1681 atgcagcgtc accagtgcca caaccgtgac cagagcagcc gtatcagtca cggtatccgt
     1741 ctgtgctgat accacctcat tttcatgtcc ggtcagcgca ttttccgggc tgacagacgt
     1801 gtcctgaccg gctgcgtcat ccgtgtcatc aagctcctct tccagctctg ccacacggag
     1861 cgccagttct tctttcgtcc ccgtcaggct gacatcacgg ttcagttgtt cacccagcga
     1921 gcggagacgg gcaatcagtt catctttcgt catggactca tccacagaga aacaatggcc
     1981 ccgaagggcc atgattacgc cagttgtaca gacacgaact catcagggtc agccagcagc
     2041 atcagcggtg ctgactgaat catggtgaac tctcgcgccg gatcgccggt agtcacccag
     2101 tttttcgggt aacgggcaga ggcgttaatg ccttcgcgct gtgcgtccgc atcctgaata
     2161 cagccatagg tacgcagacc gcgtgcctga gtgttcccca gcaccatcgt gttgtccggc
     2221 aggaagttct ttttgacgcc gttttccacg tactgtccgg aatacacgac gatggccaca
     2281 tcgccataca ttcccttata agacaccgct ttgcccaggt cttttaccgc tgtctccagc
     2341 tcggaatgag agccgcgacg ggtatccagc ttatccctga cggctttgaa ggaacggaac
     2401 agcgcccatc ctttcggatc aaacacgatg atattcacca cgccgctggc gttcagcgcg
     2461 taggcttcga tatcgtcggt cgggtcatac gtggacttgt cacgcttgct ccactccgtg
     2521 ccaccggact gtgtgatgtt gtttgccgca ctgcggccca tatccacctc aaccggatca
     2581 aaggcttcac cggtcatggt gtatttgccc ttaagcacgg cagaaactgc ctgcatctct
     2641 tcgacctggg caatggccag ctcttcgtca cgcatgttct gcatgatgat gcgacggcgg
     2701 cggtaagccg ggtccgccag attctgcgga tcttcatccg gcaggcgacg cagggtcatc
     2761 tgcggattca cctcatgctt cggcttgaca tatcccggcg taaattcaga ggtggagccg
     2821 ccacgggaac ggataacctc accggaaaca atcggcgaaa cgtacagcgc catgtttacc
     2881 agtcccggaa tttgtgagag atagactttc tccgtggtga agggatagct ctcacggaaa
     2941 aagagacgca gaaacagcgg atcaaactta aatttctgct catttgccgc cagcagctgg
     3001 gcggttgtgt acatcgacat aaaaaaatcc cgtaaaaaaa gccgcacagg cggcctttag
     3061 tgatgaaggg taaggttaaa cgatgctgat tgccgttccg gcaaacgcgg tccgtttttt
     3121 cgtctcgtcg ctggcagcct ccggccagag cacatcctca taacggaacg tgccggactt
     3181 gtagaacgtc agcgtggtgc tggtctggtc agcagcaacc gcaagaatgc caacggcagc
     3241 accgtcggtg gtgccatccc acgcaaccag cttacgggtg gcggtgtcca gcatcagcgg
     3301 ggtcattgca gacgctttcg cactcaatcc gccgggcgcg gttgcggtat gagccgggtc
     3361 actgttgccc agcggctggt aatgggtaaa ggtttctttg ctcgtcataa acatccctta
     3421 cactggtgtg ttcagcaaat cgttaacggc atcagatgcc gggttacctg cagccagcgg
     3481 tgccggtgcc ccctgcatca gacgatccag cgcagtatca ctgcgcgcct gtgcactctg
     3541 tggtgctgcg gccagaatgc ggcgggccgt ttccacggtc ataccggggg tttctgccag
     3601 cacgcgggcc tgttcttcgc gtccgtgagc ctcctcacag ttgaggatcc ccataatgcg
     3661 gctgttttct gccgcaaccg cagcggtgat ctgcgcgttc acgtccggct gcgccgcgct
     3721 ggcgttttcg ccctccatcg ctggcaccac gccagtaacg tcagcctgcg aagcagtggc
     3781 tgaaacagtt gttgattgag tctctttggt cattcgccct cctgagagac gggatttacg
     3841 tgcatccagt gcatcacgca taacggtgat cgcatcggtg ctgttgacaa gttcatcagc
     3901 cagtccggca tcaatggcct cctgaccgct gtacactgca gcctcggtat ccagcacagc
     3961 ctgcacggac aggccggtat atgccgacac cttctgcgca aacatccggc gggttgcatc
     4021 catccgggac tgcagtgttt cccggacatc acccggtaga tggctgtagg ggttgccatc
     4081 caccttatgg ctgccgctgt aaatcagcgt gatttccacg ccctgtttct ccagggcagc
     4141 accgtaatta ctgtgagcca tcatgacgcc gatggagcct gtccgggcgg tctgcgtgac
     4201 cagacgccgg gaggcggcgc tggcaagcag ctgacctgca ctgcagttca tgtcgttggc
     4261 cagcgcccat accggtttta tgtcacgcac acgggcgatg atgtcagcac agtcaaatgc
     4321 tcccgccacc attccgcccg gtgtgtccat atcgagcaga atgccgtcca ccatcgggtc
     4381 gctggcagcc tgttgcagac gggcgataat gccgttgtaa ccggtcatcc ccgaatacgg
     4441 ctgcagcgcc cgcgtccggc tgaccagcgt accggacacc ggcagcacgg cgatgccgtt
     4501 catgacctga taactccggg cctgtcgtgg tccgtcatca tcaccggata acgccagcgc
     4561 cgcgggtgcc tctccggcag tcaggctgtc gccggatact gcatccgtca ggcggctgat
     4621 cccaagctgg cctgcaagcg cacaaaagaa aacccgcgca taggcgggtt caagcatcag
     4681 cggctcatta aaagccatgc tggcaatatg cgggagatta cgcagctctg ctgtcactct
     4741 tctcctcctc tgttgattgt cgcagcccgg attcaaatgc cgcagccgcc caggcgggcg
     4801 gtttaagacc ggctgcgcgg cgctccatcg tttcacggac ctgctgggca aaaatttcct
     4861 gatagtcgtc accgcgtttc gcgcactctt tctcgtaggt gctcagtccg gcttctatca
     4921 gcatcaccgc ttcctgaact tctttcagac catcgatggc catacgaccg gagcctatcc
     4981 agtcgcagtt cccccaggca ctgcgggctt cctgaaagct gaagcgcgct tttgaaggta
     5041 acgtcaccac gcggcgaacg atggcctctt ccagccagca cagaaacatc tggctcgcct
     5101 gacgggatgc gacgaatttt cgccgcccca taaagtacgc ccacgactcg ttcgcactgg
     5161 cccgtgccgt ggagtagctc atctgggcgt aattccggga aag
//
LOCUS       DAZFDP010000248         5184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFDP010000248 DAZFDP010000000
VERSION     DAZFDP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            347..607
                     /locus_tag="ACHJ1G_004936"
     CDS             347..607
                     /locus_tag="ACHJ1G_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4766627.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            604..1152
                     /locus_tag="ACHJ1G_004937"
     CDS             604..1152
                     /locus_tag="ACHJ1G_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4766628.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1152..1376
                     /locus_tag="ACHJ1G_004938"
     CDS             1152..1376
                     /locus_tag="ACHJ1G_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766629.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1373..1696
                     /locus_tag="ACHJ1G_004939"
     CDS             1373..1696
                     /locus_tag="ACHJ1G_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO4766630.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1711..4044
                     /locus_tag="ACHJ1G_004940"
     CDS             1711..4044
                     /locus_tag="ACHJ1G_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4766631.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
     gene            4950..>5184
                     /locus_tag="ACHJ1G_004941"
     CDS             4950..>5184
                     /locus_tag="ACHJ1G_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766632.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSD"
BASE COUNT         1277 a         1199 c         1311 g         1397 t
ORIGIN      
        1 gtgagctgtt tatttttggc cgttttggct cttttattaa tcaggtgttg tcttagtgct
       61 tatgatgtaa cgccctgaat gatatgggct ggttcagatt tgagctgtga tcctgcctag
      121 tgtaatagac ttttttatta ttgatgctcc ttattatatt ccataaagga attgtttgtt
      181 tttgttcgtt tcatacggtt tttagctgtt ttattttttg agaaatcaac atttcacaca
      241 aaataaataa agcatgcgcg agccgcttat ataaacataa taaggagcta cctgaatccg
      301 catggaattg cccggactgt tgtaaacata ttagagaggt agccagatga atagtatttc
      361 cgtttccacc ccggcaccag ctgctccggt gctcccggtt ccggctcagc aggagcgctt
      421 catacgatta cctgaagtga tgcacctttg cgggctatcc cgttcaacca tctacgacct
      481 tatcagccgc gatgccttcc cgcagcagat atccctgggt ggaaaaaatg tggcctgggt
      541 acagtcggag gtcagtgcat ggatggcggc gcgaattgct gcccgcgagc aaggaggcca
      601 tgcgtgaaac ttgctttgca gtctcatctt cttttttctg gcttgcgtcc tgatatcttt
      661 tccaggtata gttttcccgc tgccgcaaaa tcggctgccg ggcgtaggaa cccgagttac
      721 ttattggcga caccagacgc gccgtgcgtc tttttttacg tcgttgctca ggcacaccca
      781 tttttcgggc tgcggtgctt ataccttagc ccctttcaga taatggtggt ccgggcgggg
      841 cagccttcgg gctggccggt ttccaataag gccggtattc ctacccccgt tcgggtcacc
      901 acccatgagc gtaggaactc cggtggtggc atttaccgct acttattgga ggttgccctt
      961 atggctacga tcctcgcccc gtcacatcct caatttatat ttgtttttgc cgccgttcgt
     1021 cgtgcagaac gtaaaccacg tatcagtatg ctgcgtatca tcgccagcaa tgagcatcat
     1081 gcacgtcttt cactggtcca tgagtatgtg ctttgtcttg ctgctcgcct gcctgtccgg
     1141 gaggtgatct aatgaatcac gccgcaatct cttatgacga tattctccgc ctgaagcatc
     1201 ttcgtaacgt gggagagttt gtcactggaa tggctgttct tcaggattgc tacgaaaaac
     1261 ctgccagtgc tcaatgtgaa caactggttt ccctgattta tctgatgaca gagcagcttg
     1321 atggcgtggt acagcgttgc caggatgacc tcatgaacat ggaggtggtg caatgaaatc
     1381 acgcactctt tccctttcac ttcgggtagc gttgtatcgc cgcgcggtag cctgcgcctg
     1441 gttgagtgcc tgctatcagc aaaaccgcca tctgcagctc actctggatg aaattgaaac
     1501 cgctattgcc cgtgagcttg aaggatttta tctgcgacag cacgggcaaa caaaaggcat
     1561 ggaaatcgcc tgcgccctgc tttcagacct gatggaatac ggccctctaa tgtcctgtcc
     1621 cgcactctcc cagctcggaa tagccgtaat ggatgagtta tgcgtccgtc acatcaaaaa
     1681 gccggtttta cactgaggga gaactgcgtc atgtcaggaa tgaaagtcag tcaggccgtg
     1741 aaggctgccc gcggtcactg ggctcagatt ctgccagcgc tgggcgtgaa tatattgaaa
     1801 aatcgccatc agccttgccc ggtctgtggc ggtaaagatc gttttcgctt cgacgatcag
     1861 gaaggacgcg gaacatggtt ctgcaatcag tgcggagccg gggatggttt agcgcttgtc
     1921 accagggcac tgaatgtgga tatcagtgaa gcagctgaca ggatacatgg actgacacat
     1981 gatctgtctg tagttaattc cgaggtcagg acgttaaccg ccgataccga tagcgggaaa
     2041 gatgcagcgg cagcacttgc cgcgcgactg ctgcaagcct cgcgtgaatc tgccggaaac
     2101 acttatctta cacataaagg tttcccggag catatttgcc atgagctgac ttcagctcat
     2161 aaggccggtg gtatgatgtt ccgccccggt gatttgatcg tcccactcta taacgctgac
     2221 ggggagctgg tgaatattca gctcatcagt ggcaatggta gcaagtgttt tcttaaagga
     2281 ggtcaggtta aggaggccta tcatctgata gaagggggcg ggagttctgt aagaaggatt
     2341 tggattgcgg aaggttacgc cacggcgctt accattcatt atttgacagg agaagccgtc
     2401 atggtggcat tttcgtcggt caactttctt tctctgagca gcgttgccca taataagtat
     2461 ccgggatatc agttaattat tgcagcggac cgcgatctga atggttcagg ccagaacagg
     2521 gctgaaactg ctgcaaaagc gagtcagtgt gacattgtgc tgccaccggt ttttggtgac
     2581 tggaatgatg cgcttaccca atacggagag gaatccaccc ggaaggcaat tcttgaggct
     2641 ttgaagccac ataacgccag tcctttcgac acaatgagtg aagcagaatt cacagcgatg
     2701 agtgtcagcg aaaaagctca gagggttcgg gagcattaca gggatgcact ggcggttgat
     2761 ccgaacgggc agcttttatc ccgctacgag tctggagcgt ggaaagttat ttctcagtca
     2821 gattttgccc gagatgtcgc agcccttttc cagcgcctcg gggcaccgtt ttcctcagga
     2881 aaaattgcct cactggtgga aacattaaag ttaattgttc cgcagcagca aaatccgtca
     2941 cgtcatctga ttggttttcg taatggcgtt ctcgatacgc gaacagggct ttttagtccg
     3001 cactgtaaag agaactggct gcgtaccctg tgtgaagtcg atttcacgcc accggtaaag
     3061 ggggaaacgc ttgaaactca tgccccggca ttctggcgtt ggctggaccg ggccgccgga
     3121 cacaagccag caaaacgcga cattattctt gcagcgttat ttatggtgct ggcgaaccgc
     3181 tatgactggc agctctttct ggaagtgact ggccctggtg gaagtggcaa aagtattctg
     3241 gctgaaattg cgaccatgct tgctggagag gataacgcca cctctgcgac cattgaaacg
     3301 ctggagtccc cgcgagaacg tgcggctctg ataggttttt ctcttattcg cctgcctgat
     3361 caggagaagt ggagcggcga cggcgccggg cttaaggcta taactggtgg cgatgcagta
     3421 tctgtggacc ccaaatatca gaatgcttat tcaacacata ttccggcggt tatcttggcc
     3481 gtaaataata atcctatgcg cttcactgat cgcagtggtg gtgtgtcccg caggagagtt
     3541 attctgcatt ttcctgagca gatagcaccg gaagaacgcg atccgcagtt gaaagataaa
     3601 attgcacgag aactggctgt gattgttcgt cagctcatgc agcgtttcag cgatccgatg
     3661 agcgccagaa cattgcttca gtcgcagcag aattctgatg aggctctcac catcaagcgt
     3721 gatgctgatc cagcatttga tttttgtggc taccttgagg cattacctga caccaacggt
     3781 atgtttatgg ggaacgccaa tattgtccca cgccagccac gtacatacct ttaccatgcc
     3841 tacctcgtat acatggaggc taatggctat aaaaacaccc tgagtcttac gatgtttggc
     3901 aaagggttgc cagtcatgct gaaggagtac gggctacatt atgagaaacg gcgaactaat
     3961 cagggaatgc aaactaacct cactttgaaa gaggagagta atgcagactg gctgcccaag
     4021 tgtgatgagc caatattaaa ataacgatta actaacccgg ctgatgccgg gcttcttatt
     4081 ggaaacgcac aaatgtagag atagctgttc actcttcacc tcgctgttaa cacgtaaaca
     4141 attgatatat aagttaaaaa tggcagggtg aatagtgtgc atggttttgc gaaaaaaaag
     4201 tattttttgt agagtcaagt cccggtttgg atccgtttaa cggaacctaa ctagattgac
     4261 tatttctctg atgatttttg gataaataat caacaaaaat caaaatgtga acagagaatg
     4321 gtgggggcac aaaaaggggc ataattgaat taattataat taaatttcaa tttttacaat
     4381 aggttaaatg tgtgtgcgag tccggccttc gcaccaagtt actaaattcc ttacaataca
     4441 gttagttaga cgattaaatc tgtctctttt gcctgtaaga aatgctctgt tatgtccctt
     4501 gaaaaagccg ctgtggttga tagtggtaat tgggatgtta actcatagcg gcgtgtcgtt
     4561 ccaagcgtca aacacgtact gactcaactt gaaagctatg acgtagccac cgtctctaaa
     4621 caatcccaaa acttttagtg cagcgcattt tagcccgatg cccccattct gtcgccgtcg
     4681 cgagacgccg caggggataa acaggtttat ggtggtcacg gtacttgcag accatatgca
     4741 agctctgcca gcggttcggc ctcgctcccc ttgggtacat ggtcatcctg ataagcctca
     4801 ctaactgaca taaccctgat gaagtcgcaa gaggctccgc ttgttgtctt accaccaacg
     4861 gggctttatg gattctattg cttaatatat gattgtatca aacttagact gcataattta
     4921 aacgaacata acaatcaggc aggggaagaa tgataaagcc attttctgtg aggtatggtc
     4981 atgttgacgt tagagagcat gtccagctga atgatcttaa ttctgataca cgcatggcat
     5041 tgtggaattg cttatatctt ttcctttgga ctaacaatcg tcaaactgct acagcaacaa
     5101 aatgtgcgca gtctgtgtgg atatattatc taaatcaacc tgcggataat attcctcgtt
     5161 atgaaagtgg atacaaaagt gata
//
LOCUS       DAZFDP010000249         5093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFDP010000249 DAZFDP010000000
VERSION     DAZFDP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..275
                     /locus_tag="ACHJ1G_004942"
     CDS             <1..275
                     /locus_tag="ACHJ1G_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein A"
                     /protein_id="HFO4766633.1"
                     /translation="QLGASDPEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHA
                     QRISETTERASRKADLLRGGLTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            352..1311
                     /locus_tag="ACHJ1G_004943"
     CDS             352..1311
                     /locus_tag="ACHJ1G_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4766634.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLAVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            1316..1627
                     /locus_tag="ACHJ1G_004944"
     CDS             1316..1627
                     /locus_tag="ACHJ1G_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4766635.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTECPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            1726..1944
                     /locus_tag="ACHJ1G_004945"
     CDS             1726..1944
                     /locus_tag="ACHJ1G_004945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766636.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQTKPLNNIHEKYDYFTDTAC"
     gene            complement(2614..3660)
                     /locus_tag="ACHJ1G_004946"
     CDS             complement(2614..3660)
                     /locus_tag="ACHJ1G_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766637.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(3660..>5093)
                     /locus_tag="ACHJ1G_004947"
     CDS             complement(3660..>5093)
                     /locus_tag="ACHJ1G_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO4766638.1"
                     /translation="LKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRM
                     ILKSRQIGATWYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEV
                     DVELKGGDKIILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMAT
                     LSGLRRTYFSTPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTW
                     RQIVTLEDVVNHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVD
                     GYDDWKDWKPFAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETR
                     RVQGLEFEEQARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSS
                     KRSLVLKMLQIMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDL
                     AWATMLAVINEPIGGEGENERFTVMEF"
BASE COUNT         1300 a         1389 c         1315 g         1089 t
ORIGIN      
        1 cacagctggg ggcatcagac cctgaagccg ccgcacgcct ggcatccaca cttcagcagg
       61 accagaacac catgaaaaca cccgttaccg tgctttctga catgctgcgc gccatccgcg
      121 acgcagagca cgcacagaga atcagtgaaa ccactgaacg cgccagccgc aaagcagacc
      181 tgctgcgggg tggcctgacc agtggaaaca aaaaacagac agaaacggga ctcacgaatc
      241 ccgtaaatga gcaaaaaacg cgcagcgata tatgaagcgc gcacaaaaca ggcaaaaacg
      301 ggatttcaga atcccgtaaa cgattaatta atcaacataa ggaaaagcga catgaaaatt
      361 tgtatcgacg acggctccac caacatcaag ctggcatgga ctgagaacgg cgaacgccgc
      421 aacgccatca gcccgaacag cttcaagtcg gaatggtctg cgccgttcgg tggcacgcag
      481 cccgcgaact acatgcttga tggcgtgcgc tatggttttg atccggtcag cgatcgcttt
      541 gtccagacga ccgacacgca ataccaatac agcgatgtga atgtcattgc cattcatcac
      601 gcgctggtca aatcaggcat cacaccacag gaggtggatg tggttgtcac cctgccactg
      661 agcgagtatt tcgacacaaa cgcacagccg gacatggcca acatcaaccg caaaaaagcg
      721 aacgttatgc gcccggtgga gtaccagaac ggcgaagcat tcactatccg taacgtgcgg
      781 gttatgcctg aatccattcc ggctggcttt aaggcactgg ctgacatgag tccgtttgaa
      841 tccctgctga ttgtggattt gggcggaacc acgctggatg tggcaaaggt tcaggggcaa
      901 ctggcaggta tcagccaggt gttttgcgat ccacacgtag gcgtttctct gatggccgat
      961 gccgtactgg cggtgatggc cactaacggt atgcgcacca gtcaccacat cgccaatacc
     1021 attatcgaac atcgccatga tgaagcctgg ctgcgccagc acatccacaa tgacgcgcat
     1081 tacgccagcc tgatggcggt tattcgtgaa aaggaagaaa cactgaaaca acgcgtgatc
     1141 cgcgcgctgg cggttttttc gggttacggg cgggtgatgg ttgtcggtgg aggggcggag
     1201 attgtggcac ccgctatccg cgaagcctgc ggagttaatg cgactttcat cgcggacggg
     1261 gtgccacagt ttgctctggt taatgggctg tacgcaatgg acaaggagta aaccaatgac
     1321 gacaccaacc agacggataa gtttctatct gaagcctgcg gccgtcaaga acgaaagcga
     1381 agcatgcgcc tggctggaca gccttacacc agaagcccgc aaaagcggcc aacgcgtggc
     1441 ttttctggcc gggctggcac ttctgaaaat gaatccagca gaggcttacc gactggctgc
     1501 atgggctgac gatgaggcgt tatcagtgac acaaaccagg acagaatgcc ccgcgtcaca
     1561 gccagtatca accgcacaga taaccagtca gatggccgga aatatccggg cattatttcc
     1621 cgaataacac aacatcaggg cgcatccgcc ctgatgactt taatccggga acataaacaa
     1681 gggggacaaa atgcaacaca ttgacagaga aaaaggaaaa aaagcatgaa cagaaaacag
     1741 aaacaagagt tgaaatactt cttacgtaaa gaaattgcca ggcttgaaga tgcagagtca
     1801 caatcatcag aaattccgtt cggaatggat atcaacgacg cccgtatgct tcaggcatac
     1861 cgtatagctc aggctgcact acagacaaaa ccgttaaata acatacatga aaagtatgat
     1921 tattttacgg ataccgcttg ctgacatctg aagaacaaaa gatagcccaa cttttaggcg
     1981 acgcatggaa cctgtattta acattgccag ttgagcatcc aatgggtaga gatgaatttt
     2041 gccgggcaat tcatcattgc caaaatatga tgttggcacg cccggccatc aggacgctgg
     2101 ccaggaaggg gcaaggctat aaaagataag taaaacgccc tcctccatgg cgtttatttc
     2161 aatgcacaat agtgcacaaa tttgcacaat ttttttgaac gactttttgc ccttccggcc
     2221 cgcgttgcgg ctggatccgt caaggatccg tgcgtgcaca aaaaaacgcg ctttttctgc
     2281 gcgcaggtga cgggggaaca gcccgcgttt cagggggtaa atagcattcc ctgaacgatg
     2341 tcgcagcgac acaacagaat ggctgtattt ctcacgctga gcgtgaaaaa ggcgtgaggg
     2401 cttttgattt gatggggtga aaggtaaggc cgtcaaaatc gcactgaggc ggcgagaaca
     2461 tacagtcaac gcggtgggat tgcgtaagag tctgaccgtc gatggtggcg ataaactgga
     2521 aggcgtcgtg aaattatctg actgatacag gagctggaga gtcggggcat aaatttttta
     2581 tgccccggcg aagcagcaga caagcgaagc gcgtcaggat gtgggctggg tgtctaacag
     2641 tgcgtaaggg ttaaagcgga tcacctcttc gccaagccag tcattgatgt gcttcatggc
     2701 ctccatgacg ggcatcagct cgttaattgc gtaaacccgc gcagccttct ccacatcacc
     2761 aaacgcactt ttttcgcccg gcatcgcccc catcagttgc ggcggaacgc ggtgcgcagc
     2821 cagcacatca tcacgggatg ccgccttaac attcatgaac tcatcctttg cggtgatctg
     2881 ctggaacggc aaaatttgca ccccctcttt gcccccgttg ggcgcatgga tgagcacgtt
     2941 tttaaacgca ccaccaccac gcgcaccctg taacgtttct ttcagggagt ccatgctttc
     3001 gcggtttacc tgcgctgcac cgatgtagat gatgcacccg gcgtgggatc cattgtcgta
     3061 atacagtttt ctgaacatgt ccgccgaatg agacaggctg gccgagagta atgcgccaag
     3121 atattccggc atgccgtaaa tttcctggtt aatgtccgga ttcatcaggt ggcacacttt
     3181 gccagggcga aactgaaacg cgtccttgcc atcctgcaca taccaccatg attcaagatc
     3241 gcttccgcgt cgcatgtatt tcgccagtgc gtgccgtaat ttaagtggtt cgccgagcat
     3301 attgctccga agctcaagga atgcgttacc gaacacaaac cagtccagcg ccagcgccga
     3361 gaaatcctgc cgggaaagca gcgggtgcgg gatgtagcaa ccgagcaata cattgcgctt
     3421 aaagtaaagc gcagactgat gccaggacgt ttgccgggcg gctcttgcca gaccgtacca
     3481 gtccaccggg gtttcatacc accgcccgtt atcagcacag tacatattgt ccagcaggtc
     3541 atgcccggtc aggcgataag gaccatcaaa tgtgaatgca ctgagcgatg attctttcct
     3601 gagcgcatca gcgagatcaa tgcgtgaact catgcgcact tttttatttt ttctgctcat
     3661 cagaactcca taaccgtgaa acgctcgttt tctccttcgc cgccaatcgg ttcgttaatg
     3721 acagcaagca tggttgccca cgcaaggtcg ccgtggctga tccccctcgc tcggtccgtt
     3781 tcgtaagtga taaagccgcc cggtgttttc accttacgca cggcgttaaa ggccgcgacc
     3841 agctcgcgtt cggcgcgatc gtattcccac cgcccggcac gcattatttg cagcattttc
     3901 agtaccagcg accgttttga tgacagcgtg aaggtgtacg gaatagcagc agggaaaaac
     3961 cgcttcacta tctgataaac agcctccccg ttcccgcccg tcacatcaat gccgatgtgt
     4021 tccacgttgt agcgatacgt gaactcttca atgactctgg cctgctcttc aaactccagc
     4081 ccctgaacgc gtcgagtctc caccgttcga aaacggccac caggaacagc cggaggaacc
     4141 acaacggaca cagcgccgct gtcgccgttg ccactactgc cgtttgcgtc atacccaatc
     4201 cataccggac gattccccat cggtcgggga gcaaaaggtt tccagtcttt ccagtcgtcg
     4261 tatccgtcaa caccgcagcc aatcaggata ttcaggttaa atgccgattc cccttcgcgg
     4321 acaaactcac acatatagag attgaggaac tcgtcttcgg tgttttcatc acgaatttcg
     4381 tcgatatcgg tgtgtttcca gccgtgatta accacatctt ccagcgtgac aatttgccgc
     4441 cacgtccggt cagggcagat aagcccgtta tgcagcgttt tccagtccac agaaaaacgc
     4501 tggcgtttat gcgaggcctt tttctcgttc cagcggtcgc cgttccagta ggcgtatgcc
     4561 tcgtgcgttt cggtggatgg cgtggagaag taggtgcgcc gcagtccgct gagggttgcc
     4621 atagcgccag ccaccttgcg cagttcagca aagcgactga cccagaaaaa ttcatcaaaa
     4681 taaaaattgc ccgtatagga ctgtgccgac gcagcagaag tgccgagaaa atgcagctct
     4741 gcgccgttgg agaggatgat tttatcgccc cctttcagct ccacatcaac ttcagccgcg
     4801 gccttctgaa taatgctttt aaactggaac gcctgacgac gcgacgcaga caaaaaaatc
     4861 tggttacgct ggtaaggttg cgccacatcg tcacgcagcg ccatcagcag cgcttcctgt
     4921 gcaaaatacc aggtcgcccc aatctgtcgg gatttcagga tcatcctgtt acgtatcccg
     4981 gcttccctgc aaagggtcag ggagtcaaac cagccccgct gatgccactc cagcctgctg
     5041 atgatttttt cccgcagtgc ggcaatctgt tccggcgtga aatgattttt gag
//
LOCUS       DAZFDP010000250         5039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFDP010000250 DAZFDP010000000
VERSION     DAZFDP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /locus_tag="ACHJ1G_004948"
     CDS             <1..307
                     /locus_tag="ACHJ1G_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4766639.1"
                     /translation="KEIVISAPDGAVFRYDADAGALSASGMKTATLQASVSVKLDTPV
                     VECTNLLRTATLDVTKGGKMSGNITHSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            304..654
                     /locus_tag="ACHJ1G_004949"
     CDS             304..654
                     /locus_tag="ACHJ1G_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO4766640.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            658..1554
                     /locus_tag="ACHJ1G_004950"
     CDS             658..1554
                     /locus_tag="ACHJ1G_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4766641.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLSEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            1547..2077
                     /locus_tag="ACHJ1G_004951"
     CDS             1547..2077
                     /locus_tag="ACHJ1G_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO4766642.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDTPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            2080..2793
                     /locus_tag="ACHJ1G_004952"
                     /pseudo
     CDS             2080..2793
                     /locus_tag="ACHJ1G_004952"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            3358..4212
                     /locus_tag="ACHJ1G_004953"
     CDS             3358..4212
                     /locus_tag="ACHJ1G_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766643.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     TSGAHAPAFIRSRRDTIDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGAHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNAENTVKNIA
                     FNYIVRLA"
     gene            4212..4790
                     /locus_tag="ACHJ1G_004954"
     CDS             4212..4790
                     /locus_tag="ACHJ1G_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4766644.1"
                     /translation="MTFRMSEQSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEADKLFRIREAEETKNSLMQVASEHIAPLQDAIDLDIATEEE
                     TSLLAAWKTYRVLLHRVDTTVAADIEWPVAPQ"
     gene            complement(4834..>5039)
                     /locus_tag="ACHJ1G_004955"
     CDS             complement(4834..>5039)
                     /locus_tag="ACHJ1G_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO4766645.1"
                     /translation="IGQIDGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMS
                     FINKDIPSNCTYITKKSGAVLYK"
BASE COUNT         1310 a         1269 c         1358 g         1102 t
ORIGIN      
        1 gaaagaaatc gtgatcagcg cgccggacgg cgcggtgttc cgctacgacg cggacgcagg
       61 cgcactgagc gccagcggca tgaaaacggc cactttacag gcatccgtca gcgtgaaact
      121 ggacacgccc gtcgtggaat gcacaaacct tctgagaacg gcgacgcttg acgtcacaaa
      181 agggggaaag atgagcggca atatcacgca cagcggcggc gatttcacct caaacggcat
      241 cacagtgcat acgcataagc acggtggcgt taaaggtggc agcgattcga caggaggccc
      301 gcagtgacaa cccgctacac aggaatgaat ccggacggga cgggaaacct gaacgatatg
      361 gagcacctga aacagtcagt cagggacatc ctgatcaccc cgctggcaag ccgggttatg
      421 cgacgggaat atggcagcct tgtgcctgat ttgattgacg aacccatgaa taacacaact
      481 cgtctgcaat gcatgagtgc tgccgtgatt gcgctgacac gatgggaacc ccgcattgcc
      541 ctggacgcca tcgacgttgt ctggaaggca ggaggccgcg ccggggtgac gctgtcgggc
      601 actgtcatgc agaccatgca gaatgttgaa ttaaccatca cgctgagaga gtaaatcatg
      661 cctgctgttg acctttccca gttaccggaa cccgccatca tcgcggagcc tgattttgag
      721 gcaattctgg ctgacacaaa ggccatgatg attgcgtcct atcctgccga acagcgtgaa
      781 gccgtctccg ccgcgctgga gctggaatcg gaacccctga acgttatcgc tcaaaccatg
      841 tcttttcgtg aaatgctgtt acgccagcgg gttaacgagg gcgcacgcgc ctgcatgtta
      901 agccacggtt cagggacaaa cctggacaac ctcgcgggca atatgaacac aaagcgcctg
      961 gttatcactc cggcaacgga taccaccgac gcggtgatgg agagcgacac ctcgctgaga
     1021 ttgcgggcgc aacgggcgta tgacggcctg agtgttgctg gcccgtcagg tgcatacgag
     1081 tattttgcac gcagcgccag cggtctggtg cgtgatgcgc gggccatcag cccgtctccg
     1141 gcctgtgtga cggtttccat cctgtccact gagggcgacg gcacagcaac ggaggcgttg
     1201 cttaataccg ttcgcgccgt tctgaatgca gaggataccc gcccggtggc cgaccgcctg
     1261 accgtacaga gtgccagaat cgtgacatgg cggctgaatg caaaactgta cttttacccc
     1321 ggcccggaat ccgaacctat tctggccgcg gcggaatcgt cgttcaggaa gtggctgtct
     1381 gagcaggggc ttatcggtca ggacgtggcg ttgtcagcca ttgctgccgc actgcatgtg
     1441 cacggtgtgc aacgcgtgga gataatcgaa cccacacaaa atatggccat cagcgacata
     1501 caggcggcgc gctgtgagtc attcaccatc agcgaaggtg ggcgtaatga gtaattcact
     1561 gttaccgcca tcagccagca atttcatgcg ttgtgccgaa gctgtcggaa cgcgcattac
     1621 agacactccg gtagacctca acacgctgtg gtcgccggac acctgcccgg tgcatctgct
     1681 gccttatctc gcctgggcat tttccgttga ccgctgggat cgcaactggc cggaagagac
     1741 aaagcgacaa gtgattcgtg atgcatggct gatacaccga cacaaaggga ccatcagcgc
     1801 actgcgccgg gccattgagc cgctgggata cctcattcgt gtgtctgagt ggtgggagtt
     1861 cggcggagaa ccgggaacat ttaccgttga agtcggcacg ctggacagtg gcgtgacgga
     1921 ggaaatgtat ctggaaatgg agcggttgat tgctgatgcc cgcccggtca gccgccacat
     1981 gacagggctg aatatcattc aggagatccc gggggatatt ttcgcggcgg cagcaactta
     2041 cgacggtgaa gtcattacca tttatccgga cgattaagca tgagtaccac aacacgaaaa
     2101 tttaaaacca ttatcactga tactggtgcc aaaaaattag ctcaggcagc cgcgccagat
     2161 ggtaatcctg tccgcctgac tcacatggcc gtgggcgacg gtggcggcac gttgcccaca
     2221 ccagacagta agcagacccg tctggtgcat gaggtgtggc gacatactgt taatcgcgtc
     2281 atcctggacg caacacatca gaaccgcatt attgcggagc tggttattcc tcctgaaacg
     2341 ggaggattct ggatccggga aattggtgtg tttgatgagc acggcgattt aatcgcggtg
     2401 ggcaatactg ccgaaagtta caagccagcc gttgccgaag ggtccggacg tgcacaaaca
     2461 tttcgcacca ttctgacagt atccagcact gccaccgtgg cgcttaccgt ggataacacc
     2521 atggttatgg ccacagtgga ttacgtggat aacaaactga aagagcatga acagtcacga
     2581 cgtcacccgg atgcctcgct gaccgcaaaa ggctttgttc aactcagtag cgccactaac
     2641 agcgattctg aaacgctggc tgcaacgccg aaagcggtta aggtcgcgta tgatcttgct
     2701 aacggaaaat atacagcgca ggatgccaca acagcgcgaa aaggccttgt ccagcttagt
     2761 agtgcaacca acagtacatc tgaaacgctg gcggcaacat caaatgcagt aaaagctgcc
     2821 tatgacaatg ctgaaaaacg tctgcagaaa gctaagaatg gtgaggatat ctctgacaaa
     2881 gacaccttta cgaaaaatat cggtgcctgc cgtgcatata gtgcagagct gaatattggt
     2941 ggagatagtg aagcatggac aactgcgcag ttgatttttt ggctagagag tcagggggca
     3001 tttaaccatc cttactggat gtgcaaaggc tcatgggctt atgcaagtaa taaggtcatt
     3061 acagatacag gttgcggaaa tatttgtctt gcaggtgctg ttgtggaagt tattggcacc
     3121 cgcggcgcaa tgaccatacg cattaccaca ccgagtacat ccagcggtga aggcatccct
     3181 aatgctcaat ttacttatat taatcatggt gatgcttatg ctcctggctg gcgaagggac
     3241 tataactcca ggaataagcc aacagcatca gagatcgggg cgttaccgtc agatgggaca
     3301 gcagtatcgt cagttaatct ggcttcaaaa ggtcggctga ccgccctgac agataatatg
     3361 cagggggcca caggtctgga gttatacgag gcgtataaca acggatatcc aacaacgtat
     3421 ggaaatatca ttcacctgaa agggatgaca gccgttggcg aaggtgagtt actcatcggc
     3481 tggagtggta caagcggtgc tcatgctccg gcatttattc gttcacgacg ggatacgatc
     3541 gacgcaaact ggtcgccgtg ggcgcagctt tacacctcgg ctcatcctcc tgcagagttt
     3601 tatccagtcg gtgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     3661 atgcaggggc agacttttga caaatctgca tacccgaaac ttgcagccgc ttatccgtca
     3721 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     3781 gtattgtctc aggaacagga cggcataaaa tcgcacaccc acagcgccag cgcatccagt
     3841 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gtactaaaac gaccagttca
     3901 tttgattacg gcacaaaaac cacaaatagc gcaggagctc attcacacaa tatacctgtt
     3961 ggtcacactg gcgcggggaa tggtgtatca gccggttata acgctgcgtt aggtactggt
     4021 accacgtcga gcgcaggaga gcatgctcac aatgtatata tcggtgccca taaccacact
     4081 atcggcattg gtgctcatgc ccattctgtc attattggtc cccacggaca caccatcacc
     4141 gttaatgcta cggggaacgc agaaaacacc gtaaaaaaca tcgcatttaa ctatattgtg
     4201 aggcttgcat aatgacattc agaatgagtg aacaatcacg gaccataaaa atttataatc
     4261 tactgaccgg aaccaatgag tttattggtg aaggtgatgc atacattcca cctcatacag
     4321 gtctgcctgc aaacagtacc gatattgccc cgccagatat tccggctggc ttcgtggccg
     4381 ttttcaacag tgatgaggca tcgtggcatc tcgttgaaga ccatcggggt aaaacggttt
     4441 atgacgtggc atcaggggac tcgttattta tttctgaact cggtccgtta ccggaaaatg
     4501 ttacctggtt gtcgccgtat ggagagtatc agaagtggaa cggcacatcc tgggtgaaag
     4561 atgcagaagc agacaaacta tttcggataa gggaggcgga agaaacaaaa aacagcctga
     4621 tgcaggtagc cagtgagcat attgcaccac ttcaggatgc tatagattta gatattgcga
     4681 cggaggaaga gacatcgtta ctggctgcat ggaagacata tcgagtattg ttgcatcgtg
     4741 ttgatacaac agtagcagca gatattgagt ggccagtcgc cccacaataa aaagaaaaag
     4801 ccatcgatag aaatatcgat ggctttatgt actctattta tacaatacag caccgctctt
     4861 tttagttata tatgtgcagt tcgatggtat atctttattt ataaaagaca ttgcacctat
     4921 ttttacatta tccccaattt tacgggataa tccaatgatg caacaattag ctccgatatc
     4981 aacgttacta ccaattttta ctcttgaacc aggcatgtca ccatctatct gtccaatgg
//
LOCUS       DAZFDP010000251         4959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFDP010000251 DAZFDP010000000
VERSION     DAZFDP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1805
                     /locus_tag="ACHJ1G_004956"
     CDS             <1..1805
                     /locus_tag="ACHJ1G_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4766646.1"
                     /translation="AIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNS
                     MAYGEGLPLPETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLN
                     HPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAS
                     GASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQF
                     GALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEK
                     NIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWAR
                     RGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEG
                     SLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQI
                     RCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYH
                     NAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAF
                     AADKLHVTDITRNATYPVLIDSIAVEVNSTDTAA"
     gene            1941..2120
                     /locus_tag="ACHJ1G_004957"
     CDS             1941..2120
                     /locus_tag="ACHJ1G_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4766647.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            2161..2406
                     /locus_tag="ACHJ1G_004958"
     CDS             2161..2406
                     /locus_tag="ACHJ1G_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4766648.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            2484..2699
                     /locus_tag="ACHJ1G_004959"
     CDS             2484..2699
                     /locus_tag="ACHJ1G_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4766649.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2704..3237
                     /locus_tag="ACHJ1G_004960"
     CDS             2704..3237
                     /locus_tag="ACHJ1G_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766650.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            3512..4081
                     /locus_tag="ACHJ1G_004961"
     CDS             3512..4081
                     /locus_tag="ACHJ1G_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4766651.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            4081..4230
                     /locus_tag="ACHJ1G_004962"
     CDS             4081..4230
                     /locus_tag="ACHJ1G_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766652.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            4458..4643
                     /locus_tag="ACHJ1G_004963"
     CDS             4458..4643
                     /locus_tag="ACHJ1G_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4766653.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT         1273 a         1113 c         1461 g         1112 t
ORIGIN      
        1 aggccattgc ggcggaaggt gatgtcacca cacctgtgtt ggtgaagccg tcggatggag
       61 aaggcacagt aatcagcgcc accagagacc cggagtatta ctttgttgtg gttctggcgg
      121 ggcagtcaaa cagcatggca tatggtgaag gccttccgct gccggagaca tatgaccgtc
      181 cggacccgcg cattaagcag ctggcgcgtc gcagtacggt gacaccgggc ggtgtcgcct
      241 gtaaatataa cgacatcatt ccggcggacc attgtctgca tgatgtgcag gacatgagcc
      301 gccttaacca tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg gggcaagggc
      361 tgcatatcgc caaaaaattg ctgccgttta taccggcgaa tgcgggcatt ctgctggttc
      421 cgtgctgtcg tggtggttca gcgttcacca ccggagccga tggcacatac agtgacgcga
      481 gtggtgcctc ggagaattca acccgctggg gtgtggacaa gccgctgtat aaggacctta
      541 tcggtcgaac aaaagcagca ctgaagaaga atccgaaaaa tgtgctgttt gccgtggtgt
      601 ggatgcaggg ggaatttgat tttggcggta cgccggcaaa tcacgcagca cagtttggtg
      661 cgctggttga taaattccgt gcagacctgg cggatatggc aggtcagtgc gtcggtggct
      721 ctgctggcgg tgttccctgg atatgtggag atacgacgta tttctggaag cagaagaacg
      781 aatccacgta ccagacggtg tacggcagct ataaaaacaa aacggaaaag aatatccatt
      841 tcgtaccgtt catgaccgat gagaacgggg tgaatgtgcc gacgaacaaa ccggaagaag
      901 acccggacat tccgggtatc ggatattacg gttcgaaatg gcgtgacagc tcagccacct
      961 ggacgtcaca ggacagggcg agccatttca gcgcctgggc acgccgtggg attatttccg
     1021 accgtctggc aacggcgatt ttgcgccatg cgggaagagt ggcgctaaac gcgggggcat
     1081 catcgacagt atcagaggtg cgcccttcat cgccttccgg tgcagaagcc acaggcgtca
     1141 caacactgct ctcttacctt gccagcgagt cagagggaag cctgaaagta cagggatggt
     1201 cagccagtgg cggcagggca gaagtggtca gcgatgcgga gggaaccgga ggtaaggcag
     1261 tgaagctgac caaggaggcc ggtaaaagca gctgggtgct ggagtacgcc gcgggcaacg
     1321 gtgcggctct gttacagaaa ggggggcaga ttcgctgccg ctttaaggtt tcgggagcgc
     1381 tggctgcgaa ccagtatgtt atggcgtttt actggccggt ctcttcactg ccacagggcg
     1441 ttgccctgac cggagacggg gggaataacc tgctggcagc gttctacatc cagacagatg
     1501 caaaagacct gaatgtgatg taccacaatg cgaaagtggc gacaaacaac ctgaaactgg
     1561 gaacctttgg cgcatttgat aacgaatggc atacgctggc tttccgcttt gccgggaata
     1621 acagccttca ggtgacgccg gttattgatg gtcaggatgg cacaccgttc acgctgacgc
     1681 agtcaccggt cagtgcattt gcggcggata aactgcatgt gacagacatt accaggaatg
     1741 cgacttaccc ggtactgata gacagcattg cggtggaagt gaacagcaca gacactgcgg
     1801 catgataaaa aaaaccgcca gcgacaggaa tggacgctgg cggtggtgat acctatggag
     1861 aaaaaataaa ggaacgatac tttcgtactc tggtttttaa tgaaaacagt tcttattgtc
     1921 aacaataacg gaaagaaatt atgacatttc tgaaccagtt aatgctgtac ttctgtacgg
     1981 tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc agtgcgcgat tgctggcgca
     2041 ggcagattga caaaagggcc gctgagaaaa tcagcgccgg tcagtcagcc ggaagcaaac
     2101 ccgaagagcc gctcatttag cggcaacttt cttaatcaca cctttcgacg agaaaatccc
     2161 atgtcagaaa ttacatccct ggtcactgct gaagcagtga aggaagtcct gcgctctgaa
     2221 gaagtccgga gcgcactgaa acagaaactt cgccataacc tggaagcgcg tcttgatgca
     2281 gaagtggatg ccattctgga tgaactgctg ggcgcaccgg cagctccgga gccggaaggc
     2341 atcgcgggtg aggggagtgc ttcagatagc ggtgacccca caccggacag cgacatgatg
     2401 atgtaagcat gcgtcaggga ccatcggtgt gtgccggtgg tcttttttat tgttgtgagc
     2461 ttccggattg cgggaggcgg ggtatgaacc agatggaaaa aatcacaaca ggtgtgtcat
     2521 acaccacgtc agcggtggga acgggctact ggttcctgca gttgctggac agggtttccc
     2581 cgtctcagtg ggcggcaata ggcgtgctgg ggagtctgct gtttggtctg ctgacgtacc
     2641 tgacgaacct gtatttcaaa atcagagagg acaggcgtaa ggcggcacgg ggagagtaag
     2701 ctgatgagca ggaaactccg ctatggttta tcggctgccg ttctggcgct gattgccgca
     2761 ggtgcttctg cgcctgaaat cctcgaccag tttctggatg aaaaggaagg caaccacacc
     2821 acggcatacc gtgatggtgc gggtatctgg accatctgcc gaggcgccat catggtggat
     2881 ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa aatgcgaccg ggttaacgct
     2941 atcgaacggg ataaggcgct ggcatgggtg gagcgtaata taaaagttcc actgaccgag
     3001 ccacaaaaag caggtatcgc gtcattttgc ccctataaca ttggccccgg taagtgtttc
     3061 ccgtcgacgt tttataagcg gcttaatgca ggcgatcgca ggggagcgtg tgaggcgatt
     3121 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gttcaaacaa ctgttacggt
     3181 caggtatcaa gacgtgacca ggagagtgcg ctggcatgct ggggtatcga caggtaagca
     3241 gaatattttg ctgaaaaatg acgttggcca acgcgggtag acaacacgaa atcctgcgaa
     3301 ctggcaaaat gtaagtgaat aaagtcaaca agattgtttc atgaagaggc accgtaatgg
     3361 tgcctttgtc atttctgcgc ttcgcacaag cgtaaataaa ccaaagaacc tttcaggatg
     3421 agccctggtg gataaccggc agtggtctgg ttaaccctct ttgggctggt tattcctgtg
     3481 cgcagggttc atcactaaaa ggaaataacc gatgaatatg atgaccgtgc cgtttcacgg
     3541 cgattctctt tatgtggtta accataacgg cgaaccatac gttcccatga aacctgtcgt
     3601 tgcggggatg gggctggcct ggcaatcaca gttggctaag ttaagacagc gttttgcgtc
     3661 aactataacg gaaatcgtta tggttgctga ggatgggaaa cgacgcaata tggtgtccct
     3721 gccgcttcga aaacttgcag gctggttaca aaccatcaat cccaacaaag taaaacccga
     3781 aatccgcggc aaggtaatcc agtatcagga agagtgtgac gatgttctct atgaatactg
     3841 gacgaagggt tttgtcgtta atccccgtcg aatgagtgtg atggaagaac tcaatcaggc
     3901 ttgcgctgac atgaaacggg ataaaaacat tgccagtgtg tttgctaccg ggctgaatga
     3961 gtggaaacag gttaaatccg cgcatgtatc aaaaatccgc acattgataa acgaagcgaa
     4021 tctgctgatt gattttgtcc tggctgatac agacaaaggg aaaataacaa aggcggattg
     4081 atggagtggt ggctaatgat atcggataaa ctcataacgc tggcgaagat cctctgtgta
     4141 atcgtcggca tttcattttt agtcattctg gttgccattt tcttttccac cgcttggcga
     4201 gtcctgacgt tatcgggact ggtggggtga aagagagatg aaccgtgttc tgtgtgtggt
     4261 gattattgtc ctgctggtag cctgtggtgc acttagtctg gggctgaatc attaccgcga
     4321 taacgccata acctacaaag agcagcgcga taaaaaagtc agtgagctgg agctggcaaa
     4381 tgcaaccatt actgatatgc agcagcgcca gcgtgatgtt gctgcacttg atgccagata
     4441 ctcgagggaa ttagccgatg cgagagctga aaatgaaact ctgcgtgctg atgttgccgc
     4501 tggtcgtaag cgcctgcgca tcaacgccaa ctgtccaggc tccttgcgta aagcccccat
     4561 cacctccggc gtggataatg caaccggtcc ccgactggca gaagccgctg aacgggatta
     4621 tttcatcctc agagaacggc tgatggcaat gcagaagcaa ctggaaggag cacaggaata
     4681 tatccgtacc cagtgtatac cgtgatgttt tgttacgaag gtgttactgg taacgttaag
     4741 gtaatttaac aaagagtcag ttccggactt tatagtgtgc tcagttcatg gccaaaaacg
     4801 atttctgtga taaatatttt gaatattatt tacaggtaaa tggagtgggg cgcatggata
     4861 gaaatattac aatagagtat gaagtatatg cccgtattgt atgggcagag aaggcaaaaa
     4921 catggtaatt ccgtgtgttg ccatgatacc tgattggca
//
LOCUS       DAZFDP010000252         4947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFDP010000252 DAZFDP010000000
VERSION     DAZFDP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHJ1G_004964"
     CDS             <1..261
                     /locus_tag="ACHJ1G_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO4766654.1"
                     /translation="ENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLI
                     AAGLESNLTVYTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(403..1527)
                     /locus_tag="ACHJ1G_004965"
     CDS             complement(403..1527)
                     /locus_tag="ACHJ1G_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO4766655.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2257..2454)
                     /locus_tag="ACHJ1G_004966"
     CDS             complement(2257..2454)
                     /locus_tag="ACHJ1G_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4766656.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2520..2790)
                     /locus_tag="ACHJ1G_004967"
                     /pseudo
     CDS             complement(2520..2790)
                     /locus_tag="ACHJ1G_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3095..3283
                     /locus_tag="ACHJ1G_004968"
     CDS             3095..3283
                     /locus_tag="ACHJ1G_004968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766657.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            3611..3805
                     /locus_tag="ACHJ1G_004969"
     CDS             3611..3805
                     /locus_tag="ACHJ1G_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766658.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4148..4276
                     /locus_tag="ACHJ1G_004970"
     CDS             4148..4276
                     /locus_tag="ACHJ1G_004970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766659.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4586..4921)
                     /locus_tag="ACHJ1G_004971"
     CDS             complement(4586..4921)
                     /locus_tag="ACHJ1G_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766660.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1472 a         1124 c         1027 g         1324 t
ORIGIN      
        1 gagaataata cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat
       61 attcgacttg gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat
      121 cattcgttaa tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat
      181 tatgcctggt ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta
      241 tcaaataata actctgtgta aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt
      301 attatgggag actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt
      361 atattactca taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt
      421 gttttttaat tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat
      481 ttcagcggct acctgatctg aacagaagcg tagtgaatca tcataaagaa ggcgatttac
      541 caaatcactg gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat
      601 ttctgcatta acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata
      661 cagcgctgtc agggtatttc ctgcgccacc gtgatgaata aatccatcag ctccattaag
      721 gaatacaccc ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc
      781 agtacgagca tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc
      841 cataacccat gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat
      901 aagtaaacgt ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt
      961 ataaggaata tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact
     1021 tgggggagca acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct
     1081 gtaagcattg cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc
     1141 aacagccagc atcactgaag gaattctata tttagcagca accaatgggc ctgccggatc
     1201 aagaggggga tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc
     1261 tgccatttca tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc
     1321 ttcctgatgg cgatatccag cctctgaatc taaacctgga actgcatcaa aaacaaccag
     1381 tccagcttct gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc
     1441 aattacacga aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg
     1501 gccaataaaa agaatacgtt ttcgcattaa aaaagtccgt cttctgctta cctgtttatt
     1561 tttctatctc taagaaaaca taattaatat tatttttatg tgagcttatg cacaaaaata
     1621 accaagtata ttatcttctg gtagagattc aggatacagt ttgtaggagc tttctttttt
     1681 gaacaatgat ttgcaggaca gttatgctaa actagcggaa tcagtatcac aacaataaat
     1741 gactgatcat acctgaaatt tgaattaaca ggcatcatga atgtcaatag tctaagcggt
     1801 gactacttat atcgcttgca taaatactaa acactgacta gaaaagtatt ggataacccc
     1861 gtcactaaaa gtaacgcttc ggttatttgc tcggaatgta ttaacgccaa gattgttagc
     1921 cgttacgcct tatatcactg gcgctgacaa atgtttcttt tctaatttac tgaaccggcg
     1981 cagacacgca ggaaagcacg ccggcaccgg tgtaatgcca gttctaatca ataagaatgg
     2041 aatgacgaat cagtcaggaa gggataacaa cccgctgctg aacggaaggt atcagcagga
     2101 agatgaacgg gaatatcgcc agcaccaagg gcagggccgg gttccacaat accagcgctg
     2161 gcattttgca cattgccagc gtaccggcat tacaacacgt ttcatgctgc tgccccccag
     2221 gattcctcga ttcgttgcta catccactgc tctacttcac ttccactgct ccacttcact
     2281 tttgtaccag cggctgctgc gccccagttt gatgggttta gggaaatggc cttcactgat
     2341 caacttgtaa aaccatttgt cggtcatacc ggtgaaactg gtgatatagt tcatgtcgat
     2401 cagggaatta tcggcacgca gaaatacgct gtcgtggctt gttgtagtca acatggtcag
     2461 aatgtcctgt gagatttatg aagattgtgc gaatgcggga aatctactga gctgtgcttt
     2521 cagaactggc ctgttacggg ataccaggga ttaccggcgg ggtaacgggc ttccggatca
     2581 tacacaccac gattatcgcg gacaaaatca ctgaacgccc atatcacctc tttaagtatg
     2641 tcttcgcagt ccggtaaatg acgatccagc gccacatccc gggtggtaca gctttgatgc
     2701 gcccggtgac acaaagcccg gattgttcca gacatcctga atcaaacgcc ccagattagg
     2761 ggcgtcgaaa tatgcctctc tgaccattat attccggcgt ataggtagca ggtctgaagt
     2821 gacaatgcgt cacctgacgt taaaagtcac ccaagatgac cccagtgggg gcaacaagta
     2881 taaaaccgtt ttttgattaa gtggtcggat atgaaaaggg gaaggtttat gtgaaaaata
     2941 cctgtgtttt ctttgaaaac acacaacagt atcaggttat ttatgtttat acagtgcgtt
     3001 ccaaactgac ctgaataatt acgcaagttg caacaataaa acatttttgc cgtagtgtga
     3061 ggtactccgg taaattgctt cccaggtgca gacaatgact aacgaagaac tacaggcaat
     3121 cgcattatcc atgcttgagc gccagcgcga aaaagtacac cagcaacgag agtctttgct
     3181 taacccatct ccattcacca ggccaaaacg attggagctt gaagcattct tggacaaata
     3241 cccacggcgg cttaaatcag gcaaaaccag accaccaggc tgatagctca cagtttgttc
     3301 tccgattcaa taaaacggat aagaaccatc agcgcggtag cgtaaggcac tacagcgcat
     3361 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
     3421 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
     3481 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
     3541 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
     3601 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
     3661 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
     3721 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
     3781 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
     3841 gcatcgttat cagcgcactt gattttcttt atatccactg acgagtttgc cggtcattct
     3901 ggtatcatgc agatatttac ggcgtacaag cacactagca tgacattgta ataaggcctg
     3961 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     4021 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     4081 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     4141 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     4201 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     4261 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     4321 cccgtcgctg catatggatc agggttggca atatcgaatt gcaggctata ggcaaagtta
     4381 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     4441 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     4501 acaactgaac taatacatca gaatctgcat tatgttaaat aaatataaaa agatggttta
     4561 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     4621 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     4681 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     4741 tccttcttgt gttaatgtca gttccccttt taccagtgct gatttccaca ttccgtccaa
     4801 cagagcttat agcctttccc tggattatag cattgtccgg ctgaagttct ttttgaataa
     4861 taatagaagc actgctggca gatccagtcc gtttttcata acccactgta ctgataacca
     4921 taatctaatc agtagaaatt gagtcga
//
LOCUS       DAZFDP010000253         4943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFDP010000253 DAZFDP010000000
VERSION     DAZFDP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(603..1175)
                     /gene="hdeD"
                     /locus_tag="ACHJ1G_004972"
     CDS             complement(603..1175)
                     /gene="hdeD"
                     /locus_tag="ACHJ1G_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4766661.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1430..1762
                     /gene="hdeA"
                     /locus_tag="ACHJ1G_004973"
     CDS             1430..1762
                     /gene="hdeA"
                     /locus_tag="ACHJ1G_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4766662.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1878..2204
                     /gene="hdeB"
                     /locus_tag="ACHJ1G_004974"
     CDS             1878..2204
                     /gene="hdeB"
                     /locus_tag="ACHJ1G_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4766663.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2268..2915
                     /locus_tag="ACHJ1G_004975"
     CDS             2268..2915
                     /locus_tag="ACHJ1G_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4766664.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(2957..3487)
                     /locus_tag="ACHJ1G_004976"
     CDS             complement(2957..3487)
                     /locus_tag="ACHJ1G_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4766665.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(3643..4209)
                     /gene="slp"
                     /locus_tag="ACHJ1G_004977"
     CDS             complement(3643..4209)
                     /gene="slp"
                     /locus_tag="ACHJ1G_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4766666.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(4564..4943)
                     /locus_tag="ACHJ1G_004978"
                     /pseudo
     CDS             complement(4564..4943)
                     /locus_tag="ACHJ1G_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1507 a          962 c         1013 g         1461 t
ORIGIN      
        1 gttgttatca atttgtaaat atcaaacaaa aagttaacgt ttgcggatta ctggccaaga
       61 ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat
      121 ccgatttaaa tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt
      181 cttttaacgc attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat
      241 agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac
      301 ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc
      361 ccacagcact tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg
      421 aaatggctaa tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaatgat
      481 cactagcagg taattatttc aatgatagtg cgcaattgat ctacaacact gcgtagcgga
      541 gagagtatta atcggatcat agtcacatca agtgactatg atcctggtga caaccgggat
      601 aattattgct gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc
      661 agttcaattc ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg
      721 aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc
      781 attgaacgct gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg
      841 gcaataaatg ccgcaatggc aaaaatgccc agctccggtg cgcggatgaa gaaatagccg
      901 atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg
      961 cggttgctga ataacccgac aataagcgca atacccgagc agattaataa tgcacccact
     1021 actgtgctta aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata
     1081 aacagcagca cggcaataaa ctggattgct ctgcgatgct ttttcagcat ctccagatca
     1141 aacttcaaaa ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag
     1201 aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa
     1261 aaatttccta attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc
     1321 tgattttgat attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt
     1381 cgaaattgat tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt
     1441 attaggcgtt attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc
     1501 gcaaaaagca gctgataaca aaaaaccggt caactcctgg acctgtgaag atttcctggc
     1561 tgtggacgaa tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga
     1621 taaaccagaa gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt
     1681 tcaggcttgt actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga
     1741 caaaattaag aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg
     1801 aacaggccgg aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat
     1861 attgaatggg ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg
     1921 ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct ggcagccaat gaatccgcta
     1981 aagatatgac ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat
     2041 ggtggatgct gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa
     2101 ccgatctcac tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt
     2161 tgtatacctt caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa
     2221 aggagtagca agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat
     2281 ttattatacg tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa
     2341 tgcgcggcaa aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt
     2401 ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg
     2461 accccagccg tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca
     2521 ttctcgttcg taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg
     2581 ccgccatagg tatggttatt ggcagcggta tgtacgaact gggtatttat ggttcagtga
     2641 tgaccttgtt ggtgctggaa gtcttccatc aattaacctt ccgcctgatg aacaaaaatt
     2701 accatttaca gctgacatta gtgaacggta atacggtatc aatgctcgac tggtttaagc
     2761 agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg
     2821 ccattgatat ccagcttcat gccacaacgt cgatagaaga cctgttgcga ttattaaaag
     2881 gtatagcagg ggtgaaaggc gtttcaatta gctaatctgt gtggcagcgt agccagactc
     2941 accgtaagcc tgaaattcac accagataat caatatgctg atggcgtacc agctcattga
     3001 tccgcttaag attgaggatc atcatgatat tgtgtttatg gcagtagaac gtttttagcg
     3061 atattttcag tttctccaga atatcttcag ttgtcatttg attcgcaatg cagctcagaa
     3121 cctgatgttg cttgcgcgaa agtgacacat tcgcttcagg cacagagcag taactgccat
     3181 tgatgagtgc gacaaaatca atgatagtga tagtccgcgg aacaaaggtc accggtactt
     3241 tccccagaca gcgtttaata ttaaaggggg agaaaacaat gacatggaca ggttttaatc
     3301 gttcaatttg agtgagaaaa ttagaatgaa atacattatt cattaatgta tcaataatga
     3361 cgaaggcatt ctgatgcaac attacgtcga tctcttcttc gttctgtata tgaacgacat
     3421 tacctttact cagaatgttt ttcatcgcgg tgaagaacat cgtatccctg gtaattataa
     3481 gaaacataac cgactcctgg tgcggacatt aactttataa tcagtttgcc aaaggtaatt
     3541 aacaggacag ggcttataat aataatagtt agcttactgt atattaattt tatgtaatac
     3601 atgatgcaga tgctgagaaa gcatctgcat ctttcggtgg tgttatttga ccagctcagg
     3661 tgttacctga ctcaccgcat tggtgtagta aggcgcaccc cagcccggtt ccggccagaa
     3721 tgcaccatag ccgtaatccc acaggttata ggtggtatta accacttctc tcaaatgcca
     3781 cacctggatg ccctgcatat tcacttccag gaagttatac gggactttat tgataaagcc
     3841 tggttgttca ccctgaatgg taccgaggat ggtaacaaag tgattacgat agttcactgg
     3901 atcaaggaag ccgctttgtc tggcgagcag tcggccctga tagttggctt caatatcagg
     3961 cttcgcatag ctatccaacg gtaatacagc gatttctaac aacgtatccg ttttgccatt
     4021 gataacgttg ataaccttcc caccaaagcg cgcttgttga ccaacatata accccggctg
     4081 gttatgaaca gcaacaaaac ttttttgaat atcaggttgg ttattgcctt tgatattttg
     4141 cggaattgaa ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg caccttttgt
     4201 catgttcatg ttactatcct tatcaacaaa tattaaagcc ataatagata tttctttata
     4261 gttttcatct gattctgagg tgatatgcct gatagtaacg taaacctgtc tgatgtttat
     4321 atcccttttg tttagttcaa ttccattgat tttggggcgt ggcatgagta tccatcgccc
     4381 cataatataa tcctttgttt taaaattaca taaaatctat ttttattcaa taattataaa
     4441 gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag cgaattcttt
     4501 tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta ggaagtggcc
     4561 tctttaaata aggcgtgtgt gacatttttt tggggttaag cgtctcgtat tgaaatactt
     4621 cgaactgata ttcagacatt tctgcccatg tttgaatttt attgtatcga gagagtgtcg
     4681 taaaggcttt aacttgctta taacattgtt tttggaattc ttgctgatat gttaatatgt
     4741 cattgtatga aagattggtt atcctggcct ctaaaaattt aattattgtc gaagcaattt
     4801 ccgcttttgc tcgtaaaaag ttgtcattaa tggttaataa tgttgaaaat aaaccttcga
     4861 tttgtttgtc aagatattgt ccatctttat ataatttggg ggtagggtgt tctttatgta
     4921 aaaaatagtg tcacctaaat ttt
//
LOCUS       DAZFDP010000254         4888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFDP010000254 DAZFDP010000000
VERSION     DAZFDP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            94..830
                     /locus_tag="ACHJ1G_004979"
                     /pseudo
     CDS             94..830
                     /locus_tag="ACHJ1G_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1009..1305
                     /locus_tag="ACHJ1G_004980"
     CDS             1009..1305
                     /locus_tag="ACHJ1G_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4766667.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1613..1729
                     /locus_tag="ACHJ1G_004981"
     CDS             1613..1729
                     /locus_tag="ACHJ1G_004981"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766668.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1948..3381
                     /locus_tag="ACHJ1G_004982"
     CDS             1948..3381
                     /locus_tag="ACHJ1G_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4766669.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4202..4765
                     /locus_tag="ACHJ1G_004983"
     CDS             4202..4765
                     /locus_tag="ACHJ1G_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4766670.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
BASE COUNT         1451 a          924 c         1105 g         1408 t
ORIGIN      
        1 atagtacacg tattaacaga ttatgtcaga aggccccggt gatgccttct gacgatagat
       61 taatgttgta ttctattggt atctggagac catatggcaa cagttacagt atattctccc
      121 cgctgtaatt cctataaagt gtaccgttat gatcacagca cctcagatca tgagcgtttc
      181 cagtgctaat cctgcaaacg tgtatttcaa ctgaattaca tctacgaaac ccgaaaacca
      241 ggcattaaag agcaaattgt tgaaatggtt cataacggag ccggggttcg cgatagctcc
      301 agaacgctga aggtggatat caacaccgtc attctcactt taaaaaacgc tcaccacgta
      361 aagtaaccat atctccagcc gccagtgtcg atgtggcact tatctgtgaa cttgatgaac
      421 aatggagttt tgtcgaaaac aaagctcgtc agcagtggca ctggtacgcg tataagacca
      481 aagctgacgg tgtgctggct tacacttttg gtcctcgcac tgatgaaaca tgccgtgagt
      541 tgccggaatt cctgaagcca ttctccgcag gcatgataac cagagacaac cggagcagtt
      601 ataccagaga aatgccacag gataagcatc ttgtcggcaa gatatttacc cggcgcattg
      661 agcgcaacaa tctgacactt cgtactcaca tcaaacgacc tgcccggaaa acgatttgct
      721 ttttacgctc cctggaaatt cacgaaaagc cattggttca tttatcgaaa acacatgtac
      781 tactgacagg agtcattacc agggcctctt ttgctgtttt tttgccatga atgataatga
      841 gaatcgacag cgataaggga aaagtaaaat aacactaatg ctgtcgtttt ctatcatatt
      901 gatttttaat tatttattgt cttttttctc aacccatttt tttacgcaga caaatttacc
      961 cggggaatac aaactgtaac gacagttcac cacactatgg gggataaaat gccagatcaa
     1021 tccggacact gcaggactcc ttcacgcatc ttacggatgc ctgaactgag ccaattgtta
     1081 ggtatatctc gttcgacaat ttacgaaaaa atgaatccgt tatcgaaata ctacgatgca
     1141 accttcccca ggccggtacg tcttggtagt ggttctgtgg gctggcgttc atcagctatt
     1201 gacgaatggc ttactttaca tactgtacct gccagatcag cagtcaaagg tgttaatgac
     1261 gaagtgagac atacgctttc ttcagatatg accatgagca gataagtttt tgtcatcacg
     1321 tgatcacctc aggaggaaaa agaagcataa gaacaattta gccagaagtt taattgataa
     1381 acagaatttc cagcattcat tgttgaaaga gcagctattg tcgctattgt agcggtaatt
     1441 gctgctataa ccgaaacgta ttacgaaaaa ggaagcattt atgccttaca tcaaaccgga
     1501 tatgtcgtat tcagtatatg catgaccatc aatgataggg aaggcaataa ttaaagctca
     1561 atacaataaa caagtacctc tgactctggt tgcaatgtca gtattaacag cgatgtcaat
     1621 tgcctgtcag aatcaggttg acgtatgcag tcccggaaat ttacgtgggg ccgttaatat
     1681 ttacactatg gttctggaga aagggaaaag accgggggaa aggtattagt gaaataattt
     1741 ccggaggtat taagaatgag aaatatgaat gatatttcgt ggtgaataat acagtcttca
     1801 ctgaattaac cttatcggtt tattaaacat cagatctaca aatgctctgt atttctcaga
     1861 gcatcaggtc tgaatagcag ctattttcgc tattgtagcg ggatttgctg ctattttact
     1921 aacccattaa tgaaaagaga ggcttttatg tgcttacttg caccagaaaa tccttatccg
     1981 atatatgcac taccaccgct ggtgagaaat gcaataattg aaactcaaaa aaatacacag
     2041 gcacctttgg ctatggtggc aacatccgca ttaacagcga tctcaattgc ctgtcagaat
     2101 cagattgacg tgtgcagacc tggaaattta catgggcctg ttaatcttta ctctctgatt
     2161 ctggctgatt ccggtgaaag gaaaacgacc gtggataaag tgtttatgaa agcattttat
     2221 ctcagggatg aagccctggc ggatgaatac gcgaaactgg ttgagaatta cagtacagaa
     2281 aaggaaatat gggagcaaaa acaaaaagcg ctggaatcaa aatttcataa agagattcgt
     2341 gccggtaaag attacaaggc aacagaatca gagcttgaaa cgcatctgaa taaacctcct
     2401 gttccgccgc agatacgtcg aacaattttt aatgagacaa cgatagaggg aatgttaaaa
     2461 tattactccg atagcaatcg ctcttttgct cttgtatcca gtgaaggggg ggtaattttt
     2521 gacagccggg ccatgagtaa actgggaatt attaacactt tgtgggatgg aggttctctt
     2581 ttcatcgaca ggaaatcatc tcccggaatt aatttgaagg agccaagact gacgatatcg
     2641 gtgatgattc agcctgatgt ttaccacaaa ggtttttgta cgcgaaaaaa agaaattgtg
     2701 aaaacgtcag gacatcatgc aaggtttttg atgtgtcaac caacatcaac gcaggggaca
     2761 aggataataa ccggagataa ttattcatca cagtatcagg atttgtttga agagaggatc
     2821 aatgagctta ttgatgaaag tctggcgatg agtggtgaac gacgttgcct tcacttttct
     2881 cctcaggctg ccagaatctg gacggattat tacaatgatg tggaatcgaa gctgggggga
     2941 ttggggcctt taagacattg ccgggaatat gctgctaaaa atgcagagta tatggcaaga
     3001 ctggcaggac ttatttacca ttcgagcggt gaagaggggg agatttcccc ttacactgca
     3061 gaaatggcga gagaattagc aatatggtac ggtaatgagt atgtgcggtt gtctaatccg
     3121 ttaacttttg acaactctgc cctgaccgta cctgtgcgac tcattccaga ggagcttgaa
     3181 cttttcaact ggataaaaag ctattgtatt gagaagggga tcctctgtat gaaaaaaaat
     3241 gacattttac agcgtggtcc gaaccgtttc cggaaaaagg ataaaatcaa ctggttactg
     3301 gatttactgt atgaacaaaa cagagttgta ccggttattg agggaaaaac gttgtgtgtc
     3361 gcacctaact ttgacctctg aacgacttaa tcgttgagta catatagtct cctctgctac
     3421 ttgcggagga gactaatgtc tttaccatgt cagcgttgta ttagaaaact ctggtacagg
     3481 tcagtcagta aatataactg gtgtaaccac tatttactat tggatagagt gttactcagg
     3541 atattgtcgg ctttagtctg aggtgaggtg gggccttcat tgttttcgtg ttcaataaat
     3601 aatttaggtt aattagtatt attagtggaa gttatgtgat ctgttatgtt gtcttgtacg
     3661 cacttaacac tggttacgta ccggaacact attagagtat gttatggctc tgttacagtt
     3721 tataggtatg aaaaaatata atctttcctg tatgaaataa tattcttatg gtgtgtgggg
     3781 tatggtctgt ataccatcat ttttatggtg aagttaaata tctgtagagg cctcaagctg
     3841 ggtcgctgtt tttacttaat ggtacagaag tattaatagc ggtgttgcct gtggctaccc
     3901 gatactggct tttgtgaatg tcagattact tcctgtaagt gattgaatgg tacttcctta
     3961 aactcaattc tatgaggtgt gtttttacca ttgtaattcg tactggtcct cagtttaagt
     4021 gagtcttact gtactctgaa atattacctc tttggagtaa tgaggtaata cgaaacagtt
     4081 ttatcaggtt gtctgtgctg ctggttacct cgtttattaa tgccgcggta cggcgtttct
     4141 gtactctcag tatttatgta attccacctt cctgtaactt ttataaagta aggactataa
     4201 aatgtataat gctaatccca attacgaaat ggacttcatg attctgaaag acgtgaatga
     4261 acacatggaa ggtctgtttc aacgtttttc taaactgtta cctttcagaa ttgattttgc
     4321 ctacaggaaa gacacgccca gttttggtca ttcctgtagg cacagtatgt gtattgaaat
     4381 gcaccgctta cttagtgaaa cccaaacgat gctggcggga tattactggg taatggaata
     4441 tacgtcaaac aagggacttc atattcattt tatcggctac ctggatgggc aacgtcacaa
     4501 caaatcttat cggatatcca ggcaactggg agatatctgg aggcggatca cggaagggga
     4561 gggttatttt cacctgtgca gggctaaaga caaatacccg gtgcgtatcg atcacgtaat
     4621 tcactatagt gataaatctg cggtagacga cttacgttac gcccttagct atctggccaa
     4681 acaggatcag aaagaacacg gtattattct gaggcgtagc aggcttcccg aaaaaagtaa
     4741 ccggggccga ccacgacata actgaattac agctgtatta ggtattagaa ttaagtttct
     4801 gtaagttgtt gggaggtgct tgagttaata ggcagaagag ggcggggcgc tttgtcatca
     4861 gtgtagggga cccgaatttc tgccgtgg
//
LOCUS       DAZFDP010000255         4884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFDP010000255 DAZFDP010000000
VERSION     DAZFDP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..358)
                     /locus_tag="ACHJ1G_004984"
     CDS             complement(<1..358)
                     /locus_tag="ACHJ1G_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4766671.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHJ1G_004985"
     CDS             complement(625..1290)
                     /locus_tag="ACHJ1G_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO4766672.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHJ1G_004986"
     CDS             complement(1431..2072)
                     /locus_tag="ACHJ1G_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO4766673.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2774..3805
                     /gene="repA"
                     /locus_tag="ACHJ1G_004987"
     CDS             2774..3805
                     /gene="repA"
                     /locus_tag="ACHJ1G_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO4766674.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1305 a         1197 c         1300 g         1082 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataaa gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtag
     2281 atcgtaatca caagatcgat tcatccaaaa aacatgaaaa acacagcata aaagatcgtt
     2341 tgtggaattg tctatttttt gacacagttc aaaaaaacga cgttttttag gtggttaatg
     2401 agaggcctgg taaatcagag aaatcgtgtt ggcttttcaa agcggtggaa aaggggtata
     2461 ttgcggatcg ttatgcagtg gcttttggga tcgtcaggat ccgggaagtc agaaaatggc
     2521 aggatgcgcc ataaggcatt caggacgtat ggcagaaacg acggcagttt gccggtgccg
     2581 gaaggctgaa aaaagtttca gaaggccata aaaggaaaac ccccataatc ttctcatcga
     2641 acttggcgga acgttagaag attatggggg cctcacagaa tacgggataa gtatatatga
     2701 aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct gtttccgcca
     2761 taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac actggagtca
     2821 gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa gggcgacctc
     2881 tttcctggtt gaaccggaaa ggaagcgcac caggcgccgt cgtggcgaac actccactaa
     2941 acccaaatgc gaaaatccgt cctggtatcg tcctgcgcgc tataaggagc tgagcgggca
     3001 gctcgggcac gcctacaacc gtctggtgaa aaaggccccg gtgaccggcg agcagagcct
     3061 gcgtatgcgt atgtcgcggc atccttttta cgtgcagaaa cggacgttcg tcggtcgcaa
     3121 atatgctttc cggccggaaa aacaacgcct cctcgatgcc atctggccgg ttctggtcag
     3181 cttcagtgat gccggcacac acactgtggg gatgagtgtc actcgcctgg ctaaagaaat
     3241 cagtccgaaa gacagtaagg gaaatgttat tccggaactg gaagtgacgg tctcccgtct
     3301 ttcccgtctg ctggccgaac aggtgcgttt tggtgtgctg ggtgtttcag aggaaacgat
     3361 gtgggaccgt gaaacccgcc agcgtctgcc gcgctatgtc tggataacac cggctggctg
     3421 gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac gactgcgtga
     3481 aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg aagatatctc
     3541 cgtacatgcg gccagaaaac gctggtatct gcagcgcagt caggatgcgc tgaaacaccg
     3601 tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg aagaaactgc ctgccgacca
     3661 gcagattcat gagatggcag agtatctcag gaagcgtctg ccgccggatg aagcctattt
     3721 ttgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc ttgaactgac
     3781 gctggctgcc ccgccaccgc actagacagc accattccct cagcactgaa tcatcgccag
     3841 cccctccggg gctttcggcg caggtatcgc tcagcccaaa atccgcagta atcaccttaa
     3901 atcccctcag aggggcatat ttgctcataa aaccacgcat cagtcatcag aacgtggcca
     3961 cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc tgcaaacctg
     4021 aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg ttgcttcgcc
     4081 gcagcgaaag tctttatctc tgaaaccact gtgaacaaac acaaagaggc cttcgcttgc
     4141 agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac gcgcgggccc
     4201 cgaacctcac cgttctggaa ccacaacaaa aaaacatcag gaataaaaac accacacaaa
     4261 cgcagcaccg tgcccacccc tcataactga aaagcgccgc cgccccggcc caaagggccg
     4321 gaacagagtc gcttttaatt atgaatgttg taactaaaca tcatcatcgc tttaagaccc
     4381 catttgtcta gcagggcgat ggcccactgc gtgaaccatc accctgcaca cagggtattt
     4441 tatgtgcaat tgtcaaccac tgaatcggaa ccccagaggg gcccccacga ttcagaagtt
     4501 gacaatttaa gcacagggac tacatgagaa aaatcgtggt tcgtctgtca tacacgcgcg
     4561 taagcggcac tgatagccct cttacgcgga aatacgccca taagcgctaa cttaagggtt
     4621 gaaccgacgc cacggtgtct cgttaagact tcaattgcgt tttgtaggca gtcagggata
     4681 ctgtcccatg aatggccacc tgtaagctcc agataaccat ttttgttatt ctccacaacg
     4741 aattccgcat accggaaatc agcgctactt ctggggagga atggccttaa gagtgataaa
     4801 gattatatgc ttttcataga gagactcatc tatgccggta acactgaaaa atgtattgtg
     4861 ttccaagaag tttttttgag tgaa
//
LOCUS       DAZFDP010000256         4815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFDP010000256 DAZFDP010000000
VERSION     DAZFDP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1995
                     /gene="mngA"
                     /locus_tag="ACHJ1G_004988"
     CDS             79..1995
                     /gene="mngA"
                     /locus_tag="ACHJ1G_004988"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO4766675.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2013..4646
                     /gene="mngB"
                     /locus_tag="ACHJ1G_004989"
     CDS             2013..4646
                     /gene="mngB"
                     /locus_tag="ACHJ1G_004989"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO4766676.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1173 a         1132 c         1340 g         1170 t
ORIGIN      
        1 agtgaaattg atcacataat ggtattgttt tatcgggcac actggcgcga ctataaaaac
       61 gatcaagtga ggatcatgat gaatctgacg actctgaccc accgcgatgc gttgtgtctg
      121 aatgcgcgct ttaccagccg tgaagaggcc atccacgcgt tgactcaacg tcttgctgct
      181 ctggggaaaa tttccagtac tgagcaattt ctggaagaag tgtatcgccg tgaaagcctt
      241 ggcccgaccg ccttaggtga agggttggct gtgccgcatg gcaaaactgc tgcggtaaaa
      301 gaagcggcgt ttgcggtcgc cacactcagc gagccgcttc agtgggaagg cgttgatggc
      361 ccggaagcgg ttgatttagt ggtgctgctg gcgattcccc ccaatgaagc gggtactacg
      421 catatgcaac tgctgacagc gctgaccacg cgcctggcgg atgatgagat tcgggcgcgt
      481 atacagtcgg cgacgacgcc tgatgagttg ctctcggcgc tggatgacaa gggaggcacg
      541 caaccttctg cctctttttc caacgcgcca actatcgtct gcgtaacggc ctgtccggcg
      601 ggtattgctc acacctatat ggctgcggaa tatctggaaa aagccggacg caaactcggc
      661 gtaaatgttt acgttgaaaa acaaggcgct aacggcattg aagggcgttt aacggcggat
      721 caactcaata gtgcaaccgc ctgtattttt gcggctgaag tcgccatcaa ggagagtgag
      781 cgttttaatg gcattcccgc gctttcagtg cctgttgccg agccgattcg ccatgcagaa
      841 gcgttgatcc aacaagcgct taccctcaag cgtagcgatg agacgcgtac cgtacagcaa
      901 gatacgcaac cggtgaaaag tgtcaaaacg gagctgaaac aggcactgtt gagcggaatc
      961 tcttttgccg taccgttgat tgtcgcgggg ggcacggtgc tggcggtcgc ggtattactg
     1021 tcgcaaatct tcgggctaca agatctgttt aatgaagaaa actcctggct gtggatgtac
     1081 cgcaagctgg gcggcgggct gctcggaatt ttgatggtac cggtgctcgc ggcctatacc
     1141 gcctattctc tggcagataa accggcgtta gcgccaggct ttgcggctgg acttgccgcc
     1201 aacatgatcg gctccgggtt tctcggcgcg gtcgttggcg gattgatagc cggttacttg
     1261 atgcgctggg tgaaaaatca cttgcgtctt ggcagtaaat tcaatggctt cctgactttt
     1321 tatctctacc cggtgctcgg tacgttggga gcgggcagtc tgatgctgtt tgtggtggga
     1381 gaacctgtcg catggatcaa taactcgctt accgcctggc tgaacggtct gtcaggaagt
     1441 aacgcgctgt tgctgggtgc cattctcggt tttatgtgtt cctttgacct tggagggcca
     1501 gtgaataaag ccgcttatgc attctgcctg ggcgcaatgg cgaacggcgt ttacggcccg
     1561 tatgccattt tcgcctccgt caaaatggtt tcggcattta ccgtaaccgc ttccacgatg
     1621 ctcgcaccgc gcctgtttaa agagtttgaa attgagaccg ggaaatccac ctggctgtta
     1681 gggctggcag gtattaccga aggggcgatc ccgatggcga ttgaagatcc gctgcgggtt
     1741 attggttcgt ttgtgctggg ctctatggta acgggcgcta ttgtcggtgc gatgaatatc
     1801 ggcctttcga cacccggtgc cggcattttc tcgctctttt tacttcatga taatggcgcg
     1861 ggcggtgtta tggcggcaat tggctggttt ggtgcggcat tggtgggggc tgcaatctcg
     1921 actgcaattc tcctgatgtg gcggcgtcac gcggttaagc atggcaacta tctgactgat
     1981 ggcgtaatgc cataaacaaa aaggaaacga cgatgaaagc ggtatctcgc gttcacatca
     2041 ccccgcatat gcactgggat cgggagtggt atttcaccac cgaagagtca cgcattctgc
     2101 tggtcaataa tatggaagag atcctgtgcc gactggaaca ggacaacgaa tacaaatatt
     2161 acgtactcga cgggcaaacg gcgatcctcg aagattattt cgcggtgaaa ccggaaaaca
     2221 aagaccgtgt gaagaaacag gtagaagccg gcaagttgat tatcggcccc tggtataccc
     2281 agaccgatac cacgattgtt tctgcggaat ccatcgtccg taatctgatg tacggaatgc
     2341 gtgactgcct cgcgtttggc gagccgatga aaataggtta tttaccggat tcctttggca
     2401 tgtccgggca gcttccgcat atctacaatg gatttggcat tacccgcacc atgttctggc
     2461 gtggatgttc tgagcgccac ggtactgata aaaccgagtt tttgtggcaa agcagtgacg
     2521 gtagcgaagt gacggcgcaa gtgttgccgc tgggctacgc catcggtaag tacttacctg
     2581 ccgacgaaga cggattacgc aagcgcctcg acagttattt tgatgtgctg gaaaaagcgt
     2641 cagtaaccaa agaaatttta ttgccgaatg gtcatgacca gatgccattg cagcaaaata
     2701 tcttcgaagt gatggataag ctacgtgaga tctaccctca acgtaagttt gtgatgagcc
     2761 gctttgaaga ggtatttgag aagatcgaag cgcagcgaga taatctggca accctgaaag
     2821 gggaatttat tgatggcaaa tatatgcgcg tgcatcgcac catcggttct acgcgtatgg
     2881 atatcaaaat tgcccacgcg cgtattgaaa ataagattgt taatctgctg gaaccgctgg
     2941 caacactggc ctggacgttg ggttttgaat accaccacgg cttgctggag aaaatgtgga
     3001 aagagatctt aaaaaatcat gcccacgaca gtatcggttg ttgttgcagt gacaaagttc
     3061 atcgcgaaat cgtcgcccgc ttcgaactgg ctgaagacat ggcggataat ctgattcgtt
     3121 tctacatgcg caaaattgcc gacaacatgc cgcagagcga cgccgacaaa ctcgtcctgt
     3181 ttaacctgat gccctggccg cgtgaagaag tgatcaacac cactgtgcgc ctgcgcgcca
     3241 gccagtttaa tttgcgggac gatcgcggcc agcctgtgcc gtattttatt cgccatgccc
     3301 gtgagatcga tccaggccta atcgatcggc aaattgttca ttatggcaat tatgatccct
     3361 ttatggagtt cgacatccag atcaaccaga ttgtcccttc tatgggctat cgcacgcttt
     3421 atattgaagc gaatcagcct ggcaacgtga ttgcggcaaa aagtgacgct gaagggattc
     3481 tggaaaatgc tttctggcag attgcgctca atgaggatgg ttctctgcaa ctggtagata
     3541 aagacagcgg tgtgcgctat gaccgggtat tgcaaattga agaaagctct gatgatggtg
     3601 atgaatatga ctattcaccc gcaaaagaag agtgggtaat taccgcggcg aacgcgaaac
     3661 cgcaatgcga tattattcat gaagcctggc agagcagggc tgttatccgt tatgaaatgg
     3721 cggtgccgcg taatctgcaa gaacgcagcg caagacaatc cactggcaga gtaggggtgg
     3781 agatggttgt cactctgagt cataacagca gacgtattga tgtggatatc aaccttgata
     3841 accaggctga cgatcatcgc cttcgcgtcc tgatccctac accttttaac accgacagtg
     3901 ttctggcaga tacgcagttt ggttcgctaa cgcgccccgt gaacgacagt gcaatgaaca
     3961 actggcagca agaaggctgg aaagaagcgc cggttccggt atggaatatg ctcaactatg
     4021 ttgccttaca ggaagggcgt aacggcatgg ctgtctttag cgaagggcta cgtgaatttg
     4081 aagtcatcgg tgaagagaag aaaacctttg ccattacgtt gctgcgtggc gtgggcttac
     4141 tgggcaaaga agatctgctt ttaaggcctg ggcggccttc gggaattaaa atgccagtcc
     4201 cggactcaca actacgtggt ctgctttctt gtcgcctaag tttattgagt tataccggta
     4261 cgccaaccgc cgctggtgta gctcagcagg cgcgagcatg gctgactcca gtacagtgtt
     4321 acaacaaaat cccatgggat gcgatgaagc tcaacaaagc cggattcaac gtgccggaaa
     4381 gttatagttt gttgaaaatg cccccagtgg gatgcctgat aagcgcactt aagaaagctg
     4441 aagaccgaca agaagtgatt ttacggctgt ttaatccggc tgaatcagca acctgtgatg
     4501 cgactgttgc tttcagtcgc gaggtgattt cttgctcaga aacgatgatg gatgaacaca
     4561 ttaccaccga ggaaaatcaa ggttcaaatc tatcggggcc ttttttaccc ggccagtcac
     4621 ggacgttcag ttaccggctt gcctgaatag caatcaaacc gaagccacat atgcgcggcc
     4681 agattgttga caaagggcgc tttgttcatg ccggatacgg catgaacgct ttattcggtc
     4741 tacaaaagca ggcaaattca atatattgca gagatgatgt agacactggc aagcgtagcg
     4801 catcaggtaa ttttg
//
LOCUS       DAZFDP010000257         4805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFDP010000257 DAZFDP010000000
VERSION     DAZFDP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..851
                     /locus_tag="ACHJ1G_004990"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHJ1G_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHJ1G_004991"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHJ1G_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHJ1G_004992"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHJ1G_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3168..3578)
                     /locus_tag="ACHJ1G_004993"
     CDS             complement(3168..3578)
                     /locus_tag="ACHJ1G_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO4766677.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3764..4732
                     /locus_tag="ACHJ1G_004994"
     CDS             3764..4732
                     /locus_tag="ACHJ1G_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO4766678.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT         1182 a         1260 c         1165 g         1198 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc gggcgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag ccccccctcc ctgctgtata ccccagtacc acaacgcggg
     3241 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     3301 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     3361 gggtcaagcc aggtgcatcg cagcaggatt ttcgcagcaa cagtcatctg gtcatgttca
     3421 aaccacggcg gttgtccgtc atttccccgc ttaagtgtta acggacaacc acaggacaca
     3481 cagcacaaaa cgtcctgtcg gttgtgcttc gcggcttctt tcgccgtaac aaaatggcct
     3541 ttgctgtcat tcgccatata gcagttcaga attcgcatat ctcctccttg tatcttgaca
     3601 attggttaaa ataactgtga ttagatttgg caaggtgtat gcagcccgat ccctcaaggg
     3661 gttcatagcc cgtagctcag cgaatggcgc atacactgag ccactcatct gaaacccgaa
     3721 cagttgtcag tacgccataa cgcatttgca aggatgggta atcatgagtc agccaggttc
     3781 actttgtgtg gatatcgatg tttcgaaagc gatactggat attgccgcca gcagcgctat
     3841 tgaacaattt tctgtcggca atgacagtga tggctttgac gccatcattg ctgagttgag
     3901 aatgtacgcc attgcgctgg ttttaatgaa agccactgga ggacttgaaa ccgccatagt
     3961 ctgtgcgctt caggcagaag gttttgagct tgccgtggtt aaccccagac aagctcgtga
     4021 ctttgcccgc gccatgggat acctcgcgaa aactgatagt attgatgcca gagtgctttc
     4081 ccagatggct gaggttctca accggcatcc ggagcgggaa cgctttatca gagcactgcc
     4141 ggatactgaa tgtcaggttc tcactgcaat ggtagttcgt aggcggcagt tgattacgat
     4201 gctggttgcc gagagaaacc ggttgcaccc tgctcatcca ttgaacagga aaagcattaa
     4261 tatcatcatc aaagcactgg aagacgagct tgcgcgacta gatgctgaca tgaacagcca
     4321 tatccatagc cacttcaaag agcttgctaa gcgactgagc agtattaaag gtgttggcac
     4381 catgacggct gctgcgctgc tggcggaagt acccgaacag ggtaacctct ccaggcgtga
     4441 aatcagtgtg cttgtcggta tggttccagt aaaccgggac tcgggcacga tgagagggcg
     4501 acgaaccatc tttggcggca gggctggtgt gcgaacagcc ctgtacatgg cagcgcttgt
     4561 cgcaacccgc tttaacccgg taataaaggt gttttacatg cgactgcttg ccgcaggaaa
     4621 acccaaaaaa gtggcgctgg ttacctgtat acgcaagctg ctgactatcc tgaacgcaat
     4681 gctcagaaag aacgaagaat ggaatgaatc gtaccatcac gttgctccat aatttttacg
     4741 ttcaagacag ttgctgagct gtccggggaa gactgaaacc tccggtctgg cagtgtgtgc
     4801 agtac
//
LOCUS       DAZFDP010000258         4744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFDP010000258 DAZFDP010000000
VERSION     DAZFDP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..567)
                     /locus_tag="ACHJ1G_004995"
     CDS             complement(<1..567)
                     /locus_tag="ACHJ1G_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4766679.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTD"
     gene            complement(638..4051)
                     /locus_tag="ACHJ1G_004996"
     CDS             complement(638..4051)
                     /locus_tag="ACHJ1G_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4766680.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRVNANAGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(4112..4684)
                     /locus_tag="ACHJ1G_004997"
     CDS             complement(4112..4684)
                     /locus_tag="ACHJ1G_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4766681.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
BASE COUNT          992 a         1466 c         1181 g         1105 t
ORIGIN      
        1 gtcagtgcgc cagtcgccac tgccggagcc ttcataagca atatcaatgg ccacggattc
       61 ggtcgggtta aactgcacgc cagctcccca cgccagagac gtgttgctgt ggcgaccgtc
      121 atcacttccg gtcagcacgt cgtgcgtttt ccccttgttg tcagttacgc ggagataatc
      181 cccggagaaa gtcgacacac ggctgtaaga cacacccgcc atcgcatacg cgctgaacca
      241 ttcattcacg cgcacagacg gccccgccat cacgctgaac cagcggttac gcacggaatc
      301 ttcatgccag cggatatcgc tgtaacgggt aagctggcga ttcttgtctc ctgcatagct
      361 gaatgacgtc accagcccca gtgtgtccgt aaactcataa cggtatttca cgttaatccc
      421 gttcagatca tcgctgccgg gaacgttcgt cgaggcatga agataccccg cgctcagcgt
      481 ggactgatgt tcagatgccc atgcagacgc accggatacg gccagacaaa tagctgcgga
      541 caaaatggct gcataaagtt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc
      601 gtcattcctg acgcccttta ttggggttat aaatatttca acgaatactg ataccggaag
      661 ccgctttttt ggtcacaatc accgtacagt cggtgatatt ccctgccccc tgattgcctt
      721 tctggaaaat cttaaactcc agagtgacgc taccaccacc actaggcata tcaataactg
      781 cactgtaact accgggaatg gcccctttag tttctctgga tgcgattaat acaccgtttt
      841 tgcgaacttc aaaaccataa cccgtgtatc ttgtacctcc cgggttatta ccacttcccg
      901 gatcgctata cgctattccg ttaaagataa tgggcggaat aatgatttga cggtcaaagt
      961 tatgatcatc gctgatggtg actgtaaccg tcccgtttgg tgtttccgta ttaccccacg
     1021 taccagcctg tttcgggaat gatttggata cagctttaac gaagtcacct ctgacctgag
     1081 tcgcctccag catgccctta atcgtacagt tttcatttac cgtgacattg ttgagcgtcc
     1141 cggcgttcgc attcactctg ccactgatat ccgcattttt agcggtcagc tttccgtctg
     1201 atgtcaggga aaataccgga ggactgccac cgctggtaat ggtgggggct gtcaggcgct
     1261 tcaggaacac gtcgttcatg aatatctggt tgccctgcgc cacaaacatc ggcgtttcat
     1321 tcccgtttgc cgggtcaata aacgcgatac ggttagcggc aaccagaaac tggctcagct
     1381 tgccttcctc cgtgtcctcc atgctgaggc caatacccgc gacataatgt ttgccgtctt
     1441 tggtctgctc aattttgacg ccccacatgg cattccattt atcgttagcg tccttccact
     1501 ctttcgaaaa ctcatccagt ctgctggcgt tatcctccgt caaatcaact ttttccagca
     1561 gctctttacc gagatgggat tcggttatct tgcctttgaa aaaatccagg taaccttccg
     1621 catcatcgct cgcccgaccg acgccctcca cgaatgccga tttgccaacg gtgttcacac
     1681 tgcggatata aaagtaataa tcatggcccg gtttgatatt gatactggcg gctatccagt
     1741 acagcgccgt accaagatag cgggctgtgg tttcaacctg cctgatatcg gtaatccgcg
     1801 tttccgagaa ccagaactca aactgtaccg tcgggtcata aacggcaaga tgcggcgtgg
     1861 cggttatctg aaaatagccc ggcgtcagct caatcctcga cggtgctgcc ggtgcggcaa
     1921 tccggaacga taccgacgcc ggatcgccct gctgccccca cgcatttacc gcccggactg
     1981 tcagcctgta gttccccagc gccagttgcg tgaagcggta tgtggtttcc gtcgtccggg
     2041 ccgtgctgac cagccgctca ctgccgtcat ccgctgttac ggtcagacgg agcaggaagc
     2101 tcacgccctt caccaccttc ggcgtgtccc agcgcgccag cacctgatat tccccgctgt
     2161 ctgcggtgac ttcggcggtc aggtgctgca ccgctggcgg cgtgacacca ttcaccgtgc
     2221 cgctctggtc gccgtcaaag tgcgccccgt tatccacgat ggcttctttt tccggtacat
     2281 gctgcacggc agtgatggca tacgtgccgt catcgttatc acggatactc acacagcgga
     2341 acaggcgctg acgcaacgtc ggcagcttca gcccccacac actgtattct gcaacgccgt
     2401 caggaacccg gttcactttc accttaagtc cgtcggtgac ggactgaacc tccacgctga
     2461 ccggattacc acttccgtca accaggctta tcagcgtggt accggaggat ggcagcgtga
     2521 tttcacggtc gagcgtcagc gtccgggtct ggctgttcac cgccagcacg cgaccaccgg
     2581 tgctgatacc ggcatagtca tcatcgaaga tttcaataac atcgcccggt acatggcgaa
     2641 gcccttcagc acccacgctg aaatccacgg tctgcgtctc cagcagttcc gttttaatca
     2701 gccacagccc ggcgcggtgt gcctgccccc ggctggtaca gccaaaggca tccatcttcg
     2761 tgacattacg accgtaacgg gcaatggcct gcgtatcttc aacaagctct gtcgccgtct
     2821 cccagccgtt gttcgggtca atccagttca cctcaacggc attatggcgg tccttcaggg
     2881 cgctgaagct gtagcggaac ggcgcgccat catccggcat caccacatta ctgcggttat
     2941 aggtccacac cttatctgat ggtcggtcct gcacgaacgt cagcgtctgc ccgttccata
     3001 ccggcataca gcgcatcgcc gagcagaaat cactgagcac atcccacgcc ttgcgctgtg
     3061 tggtcaggta tgcattacag gtgatgcgcg gctccgtgcc gccaaagccg tccggtactg
     3121 actggtcgca gtactggccg atgacataca gcgcccattt gtccacatcc gccgcaccaa
     3181 gacgtttccc catgccgtag cgtggatggg tcagcatatc ccacagacac caggccatgt
     3241 tgttgctgta tgccggttta aacgttccgt cccagatacc gctgtattgt cgcgtctgcg
     3301 ggttatagtt cgacggcacc tgcagaatgc gcccgcgaag atgataatta cgactcacct
     3361 gctggctgcc gaactgctcc aagtccacct gtacgccgac cagtgccgtg ttcgggtagc
     3421 actgtttcac atcgatgatt tcggtgtatg acgaccagag cgttttgttc tgcagctggt
     3481 ctgtggtgct gtccggcgtc atcctgcgca tccggatatt aaacgggcgc ggcggcaggt
     3541 tatccaccac caccgaggcc agatactgcg aggtggtttt gcccttaatg gtgatgtctt
     3601 tttccgtcac ccagccaccg ttacgctgta tctgaaccag caggcggact tccgacggat
     3661 tccggtcccc ctttgaggtg gtttccacca gtgcctgcac accgaaggta aagcgcagac
     3721 ggtcgatgtt tgccgacgtg atggtgcggg tgatcggcgt gtcatatttc acttccgtac
     3781 ccagcaccgt ctcggagccg gaggattcaa aaccttccgg cggtgtctgc tcctgctcac
     3841 ctgcccggaa caccaccgtg acgccggaga tgttggtatt cccctcactg tccagcaccg
     3901 gcgtactgtt cagcagcacg ctttttaatc catccaccgg accttcaatc ggcccttcgc
     3961 tgatggcatc aatcacactc agcagctgcg tggatttcag gttatctttc gcttcgcgcg
     4021 gggtatgccc cttactgctg cctttaccca ttcctcacgc tccataaacg acaaaaccgc
     4081 ccgcaggcgg tttcacataa aacattttgc atcagcgacc aatcaccaca acctgaccac
     4141 cgtccccttc gtctgccgtg ctgatctcct gagaaaccac gcgagacccc acgcgcattt
     4201 ccccgtacag aacaggcaga acattgccct gggcaaccat gttatccagt gaggagaaat
     4261 aggtgttctg cttaccgtta tccgttgtct gtgtacgggg agttctggct ttcggtgcca
     4321 gcatctgcgc cacaccaccg agcaccatac tggcaccgag agaaaacagg atgccggtca
     4381 taccaccggc cccaatggct gccccccatg ctgcaagggt ggctccggcg gtaaagaatg
     4441 atccggcaat ggctgcagcc cccaggacaa tctggaatac gccacctgac ttggccccgg
     4501 cgactctggg aacaatatga atcacagcgc catcaggcag agtctcatgt aactgcgccg
     4561 ttaacccgga cgcgctgacg tcccgcccgg caatccgtac ctgataccag ccgtcgctca
     4621 gtttctgacg aaacgccggg agctgtgtgg ccagtgcgcg gatggcttca gcccccgttt
     4681 tcacacgaag gtcgatgcgg cggccaaatc gttgcaaatc cccgtaaagg cagatgcgtg
     4741 ccat
//
LOCUS       DAZFDP010000259         4720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFDP010000259 DAZFDP010000000
VERSION     DAZFDP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..830
                     /gene="paa"
                     /locus_tag="ACHJ1G_004998"
     CDS             72..830
                     /gene="paa"
                     /locus_tag="ACHJ1G_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO4766682.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1166..1321
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004999"
     CDS             1166..1321
                     /gene="hokD"
                     /locus_tag="ACHJ1G_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4766683.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1542..1799
                     /locus_tag="ACHJ1G_005000"
     CDS             1542..1799
                     /locus_tag="ACHJ1G_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766684.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1869..2147
                     /locus_tag="ACHJ1G_005001"
     CDS             1869..2147
                     /locus_tag="ACHJ1G_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766685.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2149..3204
                     /locus_tag="ACHJ1G_005002"
     CDS             2149..3204
                     /locus_tag="ACHJ1G_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766686.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3205..3570
                     /locus_tag="ACHJ1G_005003"
     CDS             3205..3570
                     /locus_tag="ACHJ1G_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4766687.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3567..4256
                     /locus_tag="ACHJ1G_005004"
     CDS             3567..4256
                     /locus_tag="ACHJ1G_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4766688.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4452..4527
                     /locus_tag="ACHJ1G_005005"
     tRNA            4452..4527
                     /locus_tag="ACHJ1G_005005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ile,seq:cat)
     gene            4618..4691
                     /locus_tag="ACHJ1G_005006"
     tRNA            4618..4691
                     /locus_tag="ACHJ1G_005006"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4652..4654,aa:Arg,seq:tct)
BASE COUNT         1260 a         1061 c         1251 g         1148 t
ORIGIN      
        1 tctggatgta atatatatta tttgtagtgt ttataacttt atttcatgat aaccaataaa
       61 aggagttttt tatgaggaac ataatggcag gttttttaat attcctgtct tctgctgctt
      121 atgctgatat caatctgtat ggtcctggtg gcccgcatac agccttgctt gatgcagcca
      181 aactttatgc cgaaaaaaca ggtattatag tgaacgttca ttacggccca cagaacaaat
      241 ggaatgaaga tgccaaaaaa aatgcagata tcttgtttgg cgcatcagaa caatctgctc
      301 tggctatcat tcgggaccat aaagacagct tcagtgaaaa agatattcag cctctttatc
      361 tgcgaaaaag tattttactg gtaaagaaag gtaatcctaa aaatatccgg agtattgacg
      421 acctgaccag acctgggatt ggcgtaattg ttaatgatgg tggtggtacc agtaatacat
      481 caggcactgg cgtctgggaa gatattgccg gacgtaaagg gaatatagaa actgtcgccg
      541 caatccgaaa aaatattatt ttatatgcgc ccaatagcgg aactgcacgt aaggctcttg
      601 agaatcagcc tggagcagat gtctggataa cctgggctga ctgggcagcc agtaatccag
      661 aaattggtga tgtcgtggaa atagcgccag actacgtgat atggcgtgat atgaacatta
      721 cagtacgtca ggatgcaaat gatgaaaccc gtcgatttgc agaatggcta caaaccgatg
      781 aagcggcgcc tgcattcaaa aaatatggct ggaccaggaa aggcacttga catcctcgtc
      841 cttcaggacg tggattcttt ttccggatgc cgcgccagcg gcatgtaggg gcagctcaca
      901 aaacggaaaa aattgtacgc taagcctcgc caggtgaact gaattcattc cgatatggga
      961 attcccatat cgggcgaaaa cggtttgctg taacggcaga gttaagtagg attgctgcgg
     1021 gtgcttgagg ctatctgcct cgggcatgaa caccaacggc agatagataa aagccccacc
     1081 cgactataaa tcgaagtgag gcccctatat gctcgtcaca tatagattgc ctcttacgga
     1141 ccgaaaggtc aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat
     1201 cgtcatctgt ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt
     1261 acgaatccga accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta
     1321 agagaccagg cgggggagaa atccctcgcc acctctgatg tgtcaggcat cctcaacgca
     1381 cccgcactta acccgcttcg gcgggttttt gtttttattt tcaacgcgtt tgaagttccg
     1441 gacggcgccg gaatagaatc aaaaatactt aagtagcgcg cagggagaag agggatggac
     1501 cccgaacagg ggagtgctat ttatctggaa ggattctgtt gatgagaatc gaagaattac
     1561 gtgaaatttt tagtgaagat ggcctctata ctgtgcgcgt tgagaagggc gctattgtca
     1621 gccactgccg tattaaatgt ttacagtctc aacaaaggaa gagtggagct gcgttaattc
     1681 attttgtgga tgggcttgtg acggatggtt ttattttgcg tgcaaatgaa tttgtcacat
     1741 cgttgccgtc tctgaaagaa gctgggatta aggctggttt ttctgctttt gaagattagt
     1801 gaattcatct acaattcagc gcagggctga acccctgttg agtaacactg tgccaccgga
     1861 gaaagccgat ggcgcaaaat tccagactac acaattctga taattcagcc gtctttgcca
     1921 gcaggcacgg gcggcgttct catgcattca aatctgactg gttccggcac gccccatgca
     1981 ctgaagaaca ggccgaatgg ctgattcaga actaccgcag acgtgggtat gagtttagga
     2041 aagccctcag cctcgattat cgtcactgga taatctacgt caggctgccg tactccgagc
     2101 gcccaccgcg tccgtcccgc acattccagc aacgcatctg gaggtaacgt gcgggtatta
     2161 cttcgacctg ttccggtacc ggaacttggg ttggtggtcc ttaaaccagg ccgtgaatcc
     2221 atgcaggtat tccataaccc tcgagtgctt gtggagccgg aaccgaaaag catgcgcggt
     2281 ctgccgtccg gcatcgtccc tgccgttcgc cagccgctgg cggaggataa atcattactg
     2341 ccgtttttca gcaatgagcg tgtgattcgt gctgttggtg gcgctggtgc actgtctgac
     2401 tggctgttgc gtcatgtcaa atcctgccag tggcctcatg gtgactatca tcacagtgaa
     2461 accgttattc accgttatgg taccggcgca atggtgttgt gctggcactg cgacaaccag
     2521 ctgcgtgacc agacatccga atcactcgag caacttgctc atcaaaacct gtcagcatgg
     2581 atgattgacg tcatcggtca cgcaataagc ggtacgcagg agcgtgaatt atctctggct
     2641 gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga agcggtatta
     2701 cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcaa tgtaccgtga aagcgacatc
     2761 gtaccgggag agcagaccgc caccatcata ctgaagcagc gcacaaaaaa tcttgcgccg
     2821 ctgcctcacg cccaccagca aaacccgcca caggaaaaga cggtggtcag cattgccgtt
     2881 gatccggagt caccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
     2941 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gcggtaatca ggcagatgat
     3001 ccgcatcaca tcattggtca tggactggga gggatgggaa caaaggctga tgatttgttt
     3061 gttattccgc tgtgccgtaa atgccatagc gaactacacg ccggggtaaa agattttgaa
     3121 gaaaaacacg gcagccagct gttgttgctg attcgttttt taatgcacgc gagaaattcg
     3181 ggtgtcctga agtggaaagc atgaatgact gaacggatag aatttgtttt gccttacccg
     3241 ccgacggtga atacctactg gcgacgtcat ggcaatacgt atttcatctc ggaagccgga
     3301 aagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcggctgaa attaaacctg
     3361 tccggaaggc tggtgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
     3421 gacaatatcc tgaaagcacc actggatgtg ctgacgcatg ccggacttct catagacgac
     3481 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
     3541 ataaaaatca cagaactgga gtgcgcatga ataaccacta tttacagttt gtgcgtgagc
     3601 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
     3661 aggagaatgc catgttcgat acagggcgtt acaggcgaaa aaaaatccgg taccgcgatg
     3721 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     3781 cgaaggtgac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     3841 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     3901 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatactc
     3961 aatccggtac cagaaaaatt gctgggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     4021 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa tatcaggagc
     4081 tggcattact ggcgggagtg acaactaaaa actggtccaa aacatttact cgtcactggg
     4141 ttgcaatgaa acacattttt caccgactgg atagtgaggc tttattattt gtaatgagaa
     4201 cacgttcaaa acaaaaggcg gcattttcaa agcaaagtgt tgcaaaagta gattgaaagg
     4261 catatatttc atgcaaatct gatattttgc cgattttgta cgtgatggca aaagcaaaca
     4321 aaacccgccc acaagcgggt tttgttgtgc cacttatctc ggatagacat ggtgaatgcg
     4381 ctggtggagg agataagggt gatttttgta tgcttgcaac attgatttcg taacgttatt
     4441 atcctgcgcc cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg
     4501 ctggttcaaa tccagcaagg gccaccaacc gccactagcc atcaggaaag agcgtcaacc
     4561 ctttaagttg agtgtgcgag gttcgagtcc ccggtggtgg tccattatcg gtattctgcg
     4621 ttgttagctc agcaggacag agcaattgcc ttctaagcaa tcggtcactg gttcgactcc
     4681 agtacaacgc gctacactta ttttcccggc tcgcttttgc
//
LOCUS       DAZFDP010000260         4663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFDP010000260 DAZFDP010000000
VERSION     DAZFDP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..425
                     /locus_tag="ACHJ1G_005007"
     CDS             <1..425
                     /locus_tag="ACHJ1G_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFO4766689.1"
                     /translation="ISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKI
                     TITGRISASLTIENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLG
                     YFRDFYLEFVEWNESNGFYDFVYLDTENSTFNISYEEE"
     gene            425..1261
                     /locus_tag="ACHJ1G_005008"
     CDS             425..1261
                     /locus_tag="ACHJ1G_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766690.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            1340..1774
                     /locus_tag="ACHJ1G_005009"
     CDS             1340..1774
                     /locus_tag="ACHJ1G_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766691.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            1786..4635
                     /locus_tag="ACHJ1G_005010"
     CDS             1786..4635
                     /locus_tag="ACHJ1G_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4766692.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRTDIHLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTQAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQSFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPGSGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNS
                     AGNHSHNIPVGHTGAGNGVSAGFNAALGTGTTSSAGGHAHNVYIGAHNHTIGIGAHAH
                     SVIIGPHGHTITVNATGNEENTVKNIAFNYIVRLA"
BASE COUNT         1278 a         1116 c         1128 g         1141 t
ORIGIN      
        1 ttatttcagg atggcatcca aacaaaagcc agtcagagct tgaagaaatg atcctggctg
       61 cctttaagaa ggtgccgaat gagttgaaca agaaattctc gtataaagag gtcagaaaac
      121 tcccctttaa gatcaccatc actgggcgga tatcggcaag cctgaccatt gagaatgtga
      181 ccgatgagct gaagtcggca ctggaaacaa aatttgggcg cgactcaact ttctttgatc
      241 cgaaccgcgt cgggaagtac atcctgatta agaaaaaaga cgtttgggcg tttatcgaaa
      301 cgctgggtta tttccgcgac ttttatctgg aatttgtcga gtggaatgag tccaacggct
      361 tttacgattt cgtttatctg gatacagaaa actccacctt taatatttcg tatgaggagg
      421 agtgatgcag cgttcctggt ttaataaccg gcttacatca gctaagcaaa agtcattgct
      481 ctataaatca ttggctgatt tggttcagtc aatgatggac acctttgttg acccatggtt
      541 ggagcgaatt accaaccgga agtctatttt ctccatgagc aaggaggatc tggagaccag
      601 gacaaatgaa cttggccagt tctttactat cagaacctcg gactcatctt ccgttccgat
      661 gttgttacaa cagcggcttg atgagatcca ctttaaggga actgaacgtc ctataaacca
      721 gacaatttac cgcgaattta acggtatttc tgttttatgg gatccgatat atgcaccggt
      781 ggaccttgag cgtcatccct atggcacagt tctaatacca gaaagcacat tggagactac
      841 cggcggcaca ttcggcgaga tgtttctgac ttccagaggg atgatcagta ttcccataaa
      901 cgacctggcc cggacaatgg gtattactgg cacgatagat cagtccgcaa ttacagaaga
      961 aattctcaga aagtttaatc agttcgtaaa gcctctactg ccactgcata tagtgtttga
     1021 tgggcttacg ctctatttgt cggttgttgt aaatgaacag gccgacatga tcactttgaa
     1081 cgagatttct gataccgaaa aagcattctg ctggtttgaa acttcggata caacttcgct
     1141 tactggagtt acgtcgatta gcgccccgat caccgcaacg cctggtggca ctattgtgaa
     1201 agcgacacct acgtttgatc gcacccgcgc agatgatttg ttgctggata gcgacgcctg
     1261 acaatcaccc cgtccgcagg gcggggtgac aagttacttc tcttacaatg aggcttcaca
     1321 acagtgatta gggaaaatca tgtctgacgt ctcaacaaac ctctataaga gtcagttgtt
     1381 ggactattac tatcagcggc gcgctgaatc gtccattaac aaaggctctc gatttttaat
     1441 cagcaaggcc gttttcggta ccagttctct ggttactaag aaaggagatg gcacttatga
     1501 gattggagaa ctgccaaagg ctttcgatct ggcagaactg accagccaat tttgtaccat
     1561 caacctcgtg ccaacctact caggtgggat aattactgtc cgaatggacc ttgatcaaag
     1621 ccagttgcag gaagggaaaa actacccatt caacactctg gttgttctgg ataacgagaa
     1681 caagccaatc gccattattt gtgtccagga agactcgctg tatgtgggca aaacatatac
     1741 cgcagttatg gccataaaca cgactacagc ataaggatat gcttgatgaa tgacgttaca
     1801 gttgttacat cggttactta cccatcaccc gagtcgttgg ctctggttgc tgatgtgcaa
     1861 taccacgaac catatctgtc agccgcgcta aaccgaaaat tcagggggat tgttgacccg
     1921 ggattttatg ccggtttctt acctaagcct ggcggtggga tgaacctgtt aatcacctca
     1981 gtggatggag ataaaaccgc aggcgcggcg tcggtggata ttggtgaatt ctaccaggta
     2041 actattcagc aacgtacgga tattcatctt gcacttagtg caggcaagaa atatgcaatt
     2101 gtgctgaagg gaagatacct ccttggagag gatacctatc aggttaatac cgcgtcacat
     2161 attcatgcgg ctgaatttgt taccagaacc tataccgatt catatcagtt aggagatggg
     2221 gagctgcttg tttgtacggt gaatatccct gctggagtat cagccattac ccaggagatg
     2281 attgatacat ccgagcgtat taaccgcacg atcggcattg atatttcaga ctctgtaacc
     2341 agtagcagaa gtgatgttgc tgcaagttcg ctggcagtta aaaaagccta cgatctggcg
     2401 aaaagcaagt atacggcgca ggatgcaagc acaacgcaaa agggattagt tcagctcagt
     2461 agcgaaacta acagcgatag cgaaacaatg gcggctaccc ctaaagccgt taagtctgta
     2521 aaagatcttg ctgataccaa agcgccaata gaaagcccga gtctgacagg aacgccaacc
     2581 gcgccgacgg cggcgcaagg tacaaacagc acacagatcg caaatacagc ctttgttaag
     2641 gcagctataa ctgcacttat caacggtgca cctggcacac tggatacact taaagaaata
     2701 gctgctgcga tcaataacga cccgaatttc agcacaacta tcaacaatgc tctggccctt
     2761 aaagcgcctt tagcaagtcc tgcattaacg ggaataccta ctgcgcctac cgctgcacag
     2821 ggtacgaata acacgcagat tgctacgacc gcttatgtaa gagctgccat atccgcattg
     2881 gttggttcat caccagaagc tcttgatacc ctgaatgagc ttgccgcagc acttggcaat
     2941 gacccgaact ttgcgacaac aatgacaaat gcgctggcag gcaaacagcc tctggatgca
     3001 actttaaccg ctctcgctgc ccttgcgact ggtgcaaaca aactgcctta tttcactggt
     3061 aaggatacgg tagctcagac tgatttaacg tcagtcggtc gcgatattct ggctaaaaca
     3121 agcacacagg ccgttatcca ataccttggt ttaagagaac tcggtaccag cggtgaaaag
     3181 atccccctgt tgagcacggc taacacatgg agtgcgcgcc agagtttcaa cggcgggatc
     3241 accggggcgc tgacagggaa cgccgacacc gcgacgaaat taaaaacagc cataaacatt
     3301 aatggcgtca ggttcgatgg ttcggctgac attaatatca atactctggt atctcgcggt
     3361 cgcgtaacgg ccctagaggc gaatgcacag ggaacatccg ggattcagct gtatgaggca
     3421 tacaacaatg gctacccttc cccctatggc aatgtgcttc accttaaagg tgccaccgct
     3481 gctggcgaag gtgagttatt cattggctgg agtggcacga gcggtgacca tgcgcccgta
     3541 catatccgtt cgcggcgtga tactgattct gccaactggt ctgaatgggc gcaggtctat
     3601 acgtcaaaag attcaattcc cggcgtcaat gccaaagggg atcaggacac ctctggtaat
     3661 gcggctacag cgaccaagtt gcagacagca tgtactatca atggcgtctc gtttgatggt
     3721 tctactgata tcactttaac cgctgcgcat gttgctgctt ttgccagaag agcaacggat
     3781 acgtatgccg atgcggatgg gggcgttccc tggaatgccg aatcaggcgc ttacaatgtc
     3841 attcgctctg ccgacagcta tattctggtt aacttctata ccggagtcgg aagttgcccg
     3901 accttgcaga tgaaggcgca ttacagaaat ggtggtctgt tctaccgttc ttcaagagac
     3961 ggttatggtt ttgaggaaga ctgggcagaa gtttatacct cgaaaaatct tccaccagaa
     4021 agctacccag tcggcgcacc aatcccgtgg ccatcagata ccgttccgtc tggttatgcc
     4081 ctgatgcagg ggcagacttt tgacaaatct gcatacccga aacttgcagc ggcttatccg
     4141 tcaggcgtta ttcctgatat gcgtggctgg acgattaagg gcaagcccgg cagtggtcgt
     4201 gccgtattgt ctcaggaaca ggacggcatt aaatcgcaca cccacagcgc cagcgcatcc
     4261 agtacggatt tggggacgaa aaccacatcg tcgtttgatt acggtactaa aacgaccagt
     4321 tcatttgatt acggcacaaa aactacgaat agcgctggaa atcattcaca caatatacct
     4381 gttggtcaca ctggcgcggg gaatggtgta tcagccggtt ttaacgctgc gttaggtact
     4441 ggtaccacgt cgagcgcagg cgggcatgct cacaatgtat atatcggtgc ccataaccac
     4501 actatcggca ttggtgctca tgcccattct gtcattattg gtccccacgg acacaccatc
     4561 accgttaacg ctacgggtaa cgaagaaaac accgtaaaaa acatcgcatt taactatatt
     4621 gtgaggcttg cataatgaca ttcagaatga gtgaacaatc acg
//
LOCUS       DAZFDP010000261         4605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFDP010000261 DAZFDP010000000
VERSION     DAZFDP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..299)
                     /locus_tag="ACHJ1G_005011"
     CDS             complement(<1..299)
                     /locus_tag="ACHJ1G_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4766693.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVG"
     gene            complement(299..628)
                     /locus_tag="ACHJ1G_005012"
     CDS             complement(299..628)
                     /locus_tag="ACHJ1G_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766694.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(625..3237)
                     /locus_tag="ACHJ1G_005013"
     CDS             complement(625..3237)
                     /locus_tag="ACHJ1G_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766695.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSSKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAA
                     LAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(3218..3631)
                     /locus_tag="ACHJ1G_005014"
     CDS             complement(3218..3631)
                     /locus_tag="ACHJ1G_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4766696.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGIYGGVRYVPASG"
     gene            complement(3658..4080)
                     /locus_tag="ACHJ1G_005015"
     CDS             complement(3658..4080)
                     /locus_tag="ACHJ1G_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4766697.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHY
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(4094..>4605)
                     /locus_tag="ACHJ1G_005016"
     CDS             complement(4094..>4605)
                     /locus_tag="ACHJ1G_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766698.1"
                     /translation="FTLAWKPGEEGQKWLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVTAIKVTPTSGTVAKGKTTTLTV
                     SFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEV
                     TVTEAGAAG"
BASE COUNT          916 a         1545 c         1059 g         1085 t
ORIGIN      
        1 cccaccaggc tctgcaggtc ctccgccatc ccggtgacaa gaccgaacag attggacacc
       61 gtcagcgacg ggcgggcact gctgcccttt ccgttcatct caaagccgct gccctcaatc
      121 gggtacgcct gatattcacg cccctgccag gtcaccggct cccctttttc attcagctca
      181 ttgcagaaaa aataccgctc accgccctgc gccgtcaggt cgatttccca gagtaccacc
      241 cgcggtgact gctctgattt aaccgactcg ttcagacttt cttcgtgaat atcctgcatc
      301 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
      361 accacccggc acaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
      421 ccttccagcc cccgtgctct gccagaaacg cttccagatg ccgggcctcc tcccgggtca
      481 cggaaagcgt cacccggtat gttttcaggt cagcattcag ccctgccgcc atacgctgtg
      541 agtacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc tccatatccg
      601 gcttcacttt ccagcgaaag gttttcatcg cccgctccct gataacatac cgccatcacg
      661 caactgcagc cggagctcat cctgcgcccc cttgcgggcc atgtcataca ccgccttcat
      721 cagctgcggc cccgcctgtc cgttggtgcc gtcgttctga atcaccacgt gattgttctg
      781 attaaaatta atgccttccg tccgccgcat ctgcgccgga cttccggcac cgcccacata
      841 accaccttcc gcatagccgc gcatcagacg gtaaagattc cccacaccta tccggctggt
      901 tgcctctttc gtgaaaacaa actccccgcg gtgaactatc cccgcaggct catatttgcc
      961 gcccgtcccc gtaaatcctc cggtcgcgaa atggaagttc gccgccgcag cctcaatggc
     1021 cgtccccgag gaagcagatg caccaccacc gaaagcaccg ccaatggcgc tgccgatacg
     1081 cccgacaatg cccaccatgg cctgtttaag caggatttct gtcatcatgg acagcacaga
     1141 acgggtgaat ccccgccagt ctgcctctgc accggtcagc atcgccgcca tattctgtgc
     1201 aataccgtca aaggtctgcg tggcagcact tttaacctgc gaaaaactgt ccgtcgcact
     1261 ttccgcccac tcgccccagc cggacttcag cccggccatc cagcttccac gaagctgctc
     1321 ctccgcagac caggtgttct tcagtgccga tgtggccttc gccagcgcag ccggattatc
     1381 accgtacacg tcccgaagac gctgcgcttc agactcccgc tgcgcctgac ggtcagtgag
     1441 accgcgggct tttgcgctga tggcggcctg cttcgagctc tgctgctctt caaaccgcac
     1501 cgcctgctgt gccagctcat tcaggcgttt ctggtattca accttgtcgc ccagctcagc
     1561 cagctggcgt ttgtactcca gcgtctcgtc tttatgcgcc agcagggatt tttcctgctc
     1621 agataactgt cgtttcgtgg cagcctcttt caggaccgca tactgacttt ccgccttcca
     1681 taaatcacgg cgctgccggc tgattttctc attcgcaccg ctgtgttttt ccagcgtcct
     1741 gagctcagct tcaagggcaa gcagagcctc tcttgcctgc tcctcttccc tctccccggc
     1801 agagcgcgtt ttcggtgatg tatgcttttt acctgtcagc tcttcagcca gacggctgac
     1861 ggcttcctgc tgccccggac ctttgctgac gcctgttgca cgcgaacggt tgatgtaccc
     1921 catttccccc tggcgtatac gcgcatcccg ttccgcaatg gattttctca gcgccagttc
     1981 atcgcgtttt gttttctcaa taaatacgcg gttctcttct gccagttcac caaacaacgc
     2041 accaacgccg ggcacattct ttgtcgtttc ccaggctgac tgaataaatt cagccagcgc
     2101 cagatccccc tgcacaagca gcagcttcac ttgttcaacg gttccggcca ccacgtcagt
     2161 gatcagactg agtgccccca gtgtatgatc acctatccat gcccatgcgt cagaagtcca
     2221 ggttttaaca tcgtcccaga ttttttccac cggcgtggcc gctttatcaa gttgctccag
     2281 acgtgcattc atgacatccg caaacaggga catcgcctcc gtcaccgcag cctgtttacc
     2341 tttcgtgcgc tcaagctcat caatatggcg taactgggaa acgctcagga agttatactg
     2401 ctgattcagg gaggccagcg ccttcaccgg atctgctgca atcccttcaa aggctttttc
     2461 caccttcccg gcatcgtccc ccaccgtctg cagccatctc tgagaggttt cccccatgat
     2521 ccgtagctgc ccggcggtat atttcccgct ttctgccaga cgggccagat tttctgccgc
     2581 ctgtctgata ccaccaccgg cttcatcgct gatcaccccg gccattttcc acaattctgc
     2641 cgttgtggtg gcagccgccc ctccggtcag gatcagtgaa cgcaataagg cccggtcagc
     2701 ctgctctgcc tgccaggcgg cggcagcaag cgcggccagt acggcaaccc cgccacctgc
     2761 cgccacacgg gccaccgaca taaatcgtcc cagctcaccg gcattccggg cattttcagc
     2821 cagtgcattt gccgtatctg acagcgactc ctctgatgat tcagaggcat ccctgatccc
     2881 gagaagttcc tccttcagca gggtaagcag gctgagcggt ccaccgaatg aatcgctgat
     2941 ctgccccccc tgctgcagca tgataaggaa gggattctga ccaccggcaa gctgagtgac
     3001 aatatccgtg aactgtgcgg gcagtgtgcg catggcagcc ttatactgtc cgactgatat
     3061 cccggctttt tgtgccgcca gcgcctgtcg gctcagcccc tgttcaacag tactggcggt
     3121 ttttctggca tccgcctcca gactggaaaa atgacggcgt acccgggcca tctgttcatc
     3181 aaaccgggcc gcatccagac tcaaatcaat aaccagatca cccgctggct gggacatatc
     3241 tcacacctcc gtaaatcccc gctgaagcca tcattaatgc aacatcatcc tcgctgacat
     3301 ccaccacatc cgcagaaggt gaaatatcgc gccctccgtc ccctccgaac cggacgcctc
     3361 cggcaactcc tgtcgctttc tgcatcagca tttcttcctc gtccggcatc tccgtctgct
     3421 cttcctcaca cgctggagca agcagactga aatccgccgg atgcatatcc ggatcgccaa
     3481 aaaacaggct gagtacggcg tacatcagcc cggaaaaatg agcgtccagt tgggtatcct
     3541 gaaaataatg cgtgcagtaa aaacgtcgcc agtcggcata ttcggtggat gtcatcccgg
     3601 caagcatggc gcgccagtcg ggtctcccca tctctcgcgc cagtttcagg acaaagttca
     3661 gctcgccttc gaatgctttt ttgatgttac cggctcagtc gcttctgctt tcccggtttg
     3721 ttcaggatcg gcatcgtgcc ggttatccag catacctgaa agataaagca cccggttcgt
     3781 tgcctgattc agtgcatcag caggccatcc cagcatcact tcacggcgga tctgctgcat
     3841 ctctgtctcc ggagaggcca gagtgccttt gagggaatgg gaatgccata gcgacatcgc
     3901 cacaagcagg gatgccgttt ccagatatcg ctggttaatg tgcacgacat aatgcttcgt
     3961 tgtctcctgt tgttctgcgt ctgaaacaaa ctttatataa tcaaaccgct gcagcgcaga
     4021 caattcggaa agcgtgacgg acacaccgtt atattcaaat tgttctgttt tcagaaacat
     4081 gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca gccactgcgg
     4141 cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc acgccgttta
     4201 ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg gaaaccgctc
     4261 tgaacgtctt gtcggttgca ctttccggct caaaagaaac cgtcagggtg gttgttttcc
     4321 cttttgccac cgtaccggat gtcggcgtca ccttaatcgc agtgaccggc gtaattttgc
     4381 tgcgttcttc cgctacagaa ggtttgccca cgttggtcac tttcaccgtg cgggtgatca
     4441 cttctttcgc cgtcacggcc ttaccgatac tgctgaccca gccacggaac acatccaccg
     4501 tgccatttgg gaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     4561 gccatttctg accttcttct cccggtttcc aggccagcgt aaaac
//
LOCUS       DAZFDP010000262         4595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFDP010000262 DAZFDP010000000
VERSION     DAZFDP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..651)
                     /locus_tag="ACHJ1G_005017"
                     /pseudo
     CDS             complement(1..651)
                     /locus_tag="ACHJ1G_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(611..730)
                     /locus_tag="ACHJ1G_005018"
                     /pseudo
     CDS             complement(611..730)
                     /locus_tag="ACHJ1G_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(743..2353)
                     /locus_tag="ACHJ1G_005019"
     CDS             complement(743..2353)
                     /locus_tag="ACHJ1G_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO4766699.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(3028..4017)
                     /locus_tag="ACHJ1G_005020"
     CDS             complement(3028..4017)
                     /locus_tag="ACHJ1G_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766700.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(4092..>4595)
                     /locus_tag="ACHJ1G_005021"
     CDS             complement(4092..>4595)
                     /locus_tag="ACHJ1G_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766701.1"
                     /translation="SKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLIP
                     GYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQR
                     IQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESG
                     GNWMDNL"
BASE COUNT         1213 a         1057 c          976 g         1349 t
ORIGIN      
        1 gccattttta actcctctgg ataaattaaa tcacaaattt ttgtattata aataaccgca
       61 taagccaaat tatttgagat taataaaaca tcaccaactt caatatcaac aagacttctg
      121 taacatgttt tactaaatcc aattacaaaa tgaatggacc aatataatga ttttttgtct
      181 aacactggag cgtttccgta ttggcagact gtgactttat ctaaccaaag attcaattcc
      241 cctgttcata ttcttattag tggttgagca gggctcaata ctccatcaaa tactttacct
      301 acgtgcaaac gattatattt tagttcagga tgctttagat ttaacgggga actggataaa
      361 ctgagccatt cccctattaa cctttccgtt tctggcatta aagccatccc agcgagctca
      421 gatgaaatta agggaaatat ttctccaagc aatacccatc cagaccagct gccactctct
      481 tctgctatcg gtaaccattt agcactaaat tcgggcatta ataatgcaac atcttcgcca
      541 ttttctttcc aattttgaaa agtggtttca aacgtatgag tgtttcgatt tactttcctc
      601 agaccgaaca ttaactttca tccttctgat cgttttcgaa tttgctttca ttttcttgct
      661 cctcataagg ctgatgtcgt tgtccttgtc gttcatcttg ctcagtcaat acccaacgtt
      721 gtgctgtaac tcattatgaa aactatttaa ttccctcatc attgataagg ttggacatct
      781 ctttcaacat gagagtcatt gaaccattcg accaatttag cggaatgata gggcttggcg
      841 cttcatgcaa agttcccgac ttgtggatat agttatagct ttcgggaagt gccatttcag
      901 gtacactcct gccatttgca ccaatcatat tctcgcctgt tatttgggcc aatgatctat
      961 tcataaattg cactgagtca ttcaaatact cttcctttct tgattcttta taaacaattg
     1021 ctgctgattt tgcataccat gagtcaaaaa accattctgc ttctgttgat ggaatgaagc
     1081 tcttttctct tttcgcatgt gaattttgat cggtatcggt tttaatatca ttgaaccaaa
     1141 aatttgccga ctgataatta tctttctcgt atctttttat accatacgga cctgctagtt
     1201 ttttaactat ttttagaacc tgctcttttc ttctggtgtt tatctttgca agatttgcag
     1261 gataaataac attaagtaaa gccgcatcgg cttctctgta atgtttatct ttttccagat
     1321 aacctgggga ttctccccct aaatcaagct gtaacgttat tctttcataa cctttatcta
     1381 tgaggtggtt caacctcgtt gttgaaagag gttcatccag ttcattggct ttagcttcgc
     1441 gtagtaaatc agagacaaaa acagagtctt tcttcgagag taagttcgac aaacgttcca
     1501 gtccagaggt caccagtgca acggaagatg tgtttaatcg ggcatcttct tcccatgctc
     1561 cggaatcttc cataacatag aaattggctt tatccagata agcaatcagc agcgcaaccg
     1621 attttaatcg gtcacctttt tgccaatctt cagcgttaat tgtatcagtg tcgattgctt
     1681 gtataaggag atcaagataa agacctaacg catcattttg tttatggttc cacagctgcg
     1741 gcttgccttc ttcctgcaca tctgccatca cgggggagtt gctgtcaaat cggatatgca
     1801 caacattcat ttgcccggga actccatcca gtcgtttggg gttgctaatg acgtcctgca
     1861 tccgcttaat ctggtcgggc gtagacatat aatcccacag ggtgagcaga accttttttg
     1921 cggctacact atttccctga tctgatacta atgccatata gccccataaa ctatccctca
     1981 gccagacagc atcatagtgg gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa
     2041 gaataaacgt tccccagggt ttctccgtca acgttaactt tcgtgtgttt tctgttaatg
     2101 ttagcaatcc ttgaatttcg tctttggtat agtggctctt tatttcattt tgtaatttgg
     2161 ctgagtgaat caaaagacga taattaggat acttatctgg atttaacgat aaataacttt
     2221 tatctgttgc tgcccccgtg gaattaagaa tgttaaatac agattcactc gtcacttctg
     2281 cccctgtcgc ctggcatgtg ccgcacgaaa atataccagc gagcagaaga gttaatgcag
     2341 atttcccttt cataaaatat tccatcaatc gtttgttaaa agcgtagata accacttatt
     2401 taacaacttg aaaataaggt gttttacctg ggttgttaca aaaggattgc attgcgtaaa
     2461 cgctttttat ttacaacaaa atggggaagt attacggcga ttgcgaaaag tctgaagcga
     2521 ttgaattaag ataatataga aaatcagata cagcaaaaat ggcagattga tcaggtgaag
     2581 ataaattgga gcgggcgaag ggagcctacg acaaatcgaa gacaaccagc taacctcttg
     2641 tttcttaaag actgatgaat ccagttggtt gtagtgatag tcacacattt agcgatagac
     2701 ggtcaagact tgaagcacgt aatattccct ctaaaggaat agaactcacg gtctgactac
     2761 ccggcccttt tggtctgact cagggtcggg ctatggggac ttttggctcg ctctttatat
     2821 tagatgaggt ctcacatttt tcagtcaaaa ctcatcgtct aatagcccat agccagacca
     2881 ccagtcccag ctttagcagg aagaccacga gatagacttt gtgtaggtcc atttggtcct
     2941 cctcccttct gcattcacga actagattac tagtacaaaa taaaggccag acatccagtt
     3001 cggaagtctg accaatagtc gttattgtta gttagcttct ttagtgaagc tcaggcgttt
     3061 gactacaggc aggacagcag tagccgagcc agtccccatc ttgatggtcg tagcagcttc
     3121 agctacaaca aacaccttgc cgagaatctc aaggtcgtga gactgggagt caccatcacg
     3181 tgaaaggacc acagtaatgt ggtccccatc gtgacggaag caaccgaagc tcagagccgt
     3241 aaaacgctta gccatcatga agacgccacg gtcttcaccg ttgtaatagg cgatgtcagt
     3301 ccccaacata tcgacgatag cgtcttcacc accaaagccc tgcttagcag caagacgtga
     3361 cagtgccttg aggtgtgact catgggcgat gatgccgtaa tcagcaacct cagaaccgaa
     3421 agaaggctcg gtgttctcgt acagcgcgtc catgatagtg tctgccttag ctgtcaggct
     3481 accagtcagg accggaagct ctacagtctt caacagcttg gaggattcca gtacatcgac
     3541 catgcgacta ttgatggtac ggaccacgtt atcgcagatg ttcagagcgt cctgctggag
     3601 cgtcccctga gtgaaaccag tggaagactt agcgtcccat agcttggtct ggtgggacac
     3661 gaggatagct tgctcaatcg gctcaacggt gatttcagta ccagacggag cggtcacgac
     3721 ttcaggtcgc ttaccaccac ggacaggaac gaccacagag cggtcagcca gagtgtgagc
     3781 aatgattggg gtcggtaagc tattgcgagt tggcagaatc tcaatcggga atggaatgtt
     3841 atcctgagcg gacttcagca caccattcag catggtcttg acagctttag ccagtgccag
     3901 agcatagacc tgtgggtcag cattggttgg cagaccttcg ttaacctgaa tgttgtctgt
     3961 gtcggtgata cgtactggct caatgccgaa ctgatagctg tctttagtca tagtcataat
     4021 aatcaatctc ctaaattagt ttagatacgt gggtcccgac atcaggtcag gaccacttgt
     4081 gtgcgtctgt cttacaggtt gtccatccag ttaccgcctg actcaaggtc agcgacgata
     4141 gcggcacggg agcgaccagc ggaatgggta gcactacgaa ggttctcacc gtaggtccgg
     4201 cgttcagctt tgacctgttc acggcgctgt agctctttct gccactctgc atcagtcatg
     4261 ttctcttcac gcatgcgctc ggcctgaatg cgctgctctt tagcgatacg ctcagcctca
     4321 gctcgcttgt tctgctggcg gcgaccatag gcggtcatca ggtcattact gaagaagtta
     4381 ccaatggtca cgccagcttc agccagggct tccaataact ccatagactc gcggctggta
     4441 gacggcaggc ctgacaggta gcccggaatc agggtcttga tagcgaggga cagcggatag
     4501 aactgaccgt ccagactcac agtattctcg tagagattga tgtcggtgac tgacagtttc
     4561 cagtcttggt tattgaataa cgcactagct ttgct
//
LOCUS       DAZFDP010000263         4462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFDP010000263 DAZFDP010000000
VERSION     DAZFDP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..4244
                     /locus_tag="ACHJ1G_005022"
     CDS             <1..4244
                     /locus_tag="ACHJ1G_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO4766702.1"
                     /translation="IPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESN
                     AGFMDRVSAGASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPR
                     IAQGSSNVFINNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMI
                     LGGITIASYLLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSI
                     ANKVIRWVTDPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSW
                     SEYAWVREQGNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDAL
                     SSRFFYDAFPGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYR
                     ITGVSHTDGIRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLF
                     YEYDAADRIIRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKG
                     THRYYYDPDGNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRY
                     TAADGAEWLYRYDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYDHHN
                     RPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAETGAEGFR
                     YDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVINPQG
                     ESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEV
                     ITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDEHTV
                     IRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVLRHS
                     NRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDY
                     GRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD
                     QYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGE
                     QWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELI
                     SQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALS
                     LPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGL
                     QGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGKVREALDVHYEDL
                     VRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKKTIELAE
                     EQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            4246..>4462
                     /locus_tag="ACHJ1G_005023"
     CDS             4246..>4462
                     /locus_tag="ACHJ1G_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766703.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIA"
BASE COUNT         1096 a         1177 c         1378 g          811 t
ORIGIN      
        1 caatacctga aatcccacag ggggaaccct catggggtga tattgccact gacctgcttg
       61 aatcggcagc ggaaaaagca gtaccactgg cgaaggcctg ggggaacgct gttatcaccc
      121 tgacggaaag caatgccggt tttatggatc gcgtcagcgc cggcgcatcg cttctgtttc
      181 ccgccggtcc ggtattaatg gagtttgcca ccatggtggg cgggcgtagc gaaatcaaaa
      241 aagatgtgga tttcccggaa gccggtgagg acacggcgct ctgcgacaag gagaacaaac
      301 caccgaggat agcccagggc agtagcaacg tctttatcaa caatcagcct gccgcgcgca
      361 agggcgacaa actggagtgc agtgcggcga tcgtgggagg ttcgccggac gtctttattg
      421 gcggtgaaca ggtgacctat ctggatatcc agccggagtt cccgccgtgg cagagaatga
      481 tcctgggggg aataacgata gccagctatc ttctgccgcc tgcgggactg ctgggaaaac
      541 tgaagaatct ggcgagactc ggcaaactgg gaaacctgct ggggaaaagc gggaagctgc
      601 tgggcgcaaa gctcggcgcg ttgctgggga aaacaggtaa gtcgttaaaa agtattgcca
      661 ataaagtcat cagatgggta acagatcctg tcgatccggt aaccggcgca tactgcgacg
      721 aacgtaccga cttcaccctg ggccagaccc tccccctctc cttcacccgt ttccacagtt
      781 ctgtactgcc gctgcatggc ctgaccggcg tgggctggag cgactcctgg agcgaatacg
      841 cctgggtgcg tgaacaggga aatcgggtag atattatcag ccagggagcc acgctgaact
      901 tcgccttcga cggtgacagt gatacggcgg ttaacccgta tcacgcccag tacattctgc
      961 gccgccgcga cgattacctg gagctgttcg acagggatgc actgagcagc cgcttctttt
     1021 atgacgcctt tccgggaatg cgtctgcgcc acccggtgac tgacgatacc agcgatgacc
     1081 gcctggcaca cagccccgac gaccggatgt atatgctggg cgggatgagc gacaccgcca
     1141 gcaaccgcat cacgtttgag cgcgacagcc agtaccggat cacgggtgtc agtcacaccg
     1201 acgggatccg gcttaaactg acgtaccacg ccagcggcta cctgaaagcc attcaccgca
     1261 cggataacgg catacagacg ctggcgacct acgaacagga tgcgcggggg cggctgacag
     1321 aagcggatgc gcggctggac taccacctgt tttatgagta cgacgctgcg gaccggatca
     1381 tccgctggtc cgataacgac cagacgtgga gccgtttcac ctacgatgca cagggccggt
     1441 gcgtgaatgt caccggggcg gagggctatt acaacgccac gctggactat ggtgacggct
     1501 gcaccaccgt gacggacggc aagggcactc accgttatta ctatgatcct gacggcaata
     1561 ttctgcggga agaagcgccg gacggcagta ccaccacgta tgaatgggat gaattccatc
     1621 acctgctggc ccgccactcc cctgccgggc gggtggagaa gtttgaatac aacgccgcac
     1681 tcggtcagtt aagccgttac acggcggcag acggcgcgga gtggctgtac cgctatgatg
     1741 agcgcggcct gccggtaagc ctggtgtcgc cacagggcga agagacccgg ctggcataca
     1801 ccgctcaggg gctgctgtcg gggatattcc gccaggatga acggcgtctg ggcatagagt
     1861 acgaccacca caaccggccg gaaacactca ccgacgtgat gggccgtgaa caccacaccg
     1921 aatacagcgg tcacgacctg ccggtgaaga tgcgcggccc cggcggtcag tcagtgcggt
     1981 tacagtggca gcagcaccat aaactgagcg gccttgagcg ggcagaaacc ggcgcagaag
     2041 gattccgcta tgaccgccac ggcaacctgc tggcgtacac ggacggtaac ggcgttgtct
     2101 ggacaatgga atacggcccg ttcgatttgc cggtggcgcg aacggacggt gaaggccacc
     2161 gctggcagta ccgctacgat aaagacacgc tgcagctcac agaagtcatt aacccgcagg
     2221 gcgagtcata ccgttatatt ctggacaact gtggccgggt gacggaagag cgtgactggg
     2281 gcggcgtggt ctggcgttac cgctatgacg ctgatggcct gtgtaccgcc agggtcaacg
     2341 gcctggagga aaccatcctc tacagccggg acgccgcagg ccgcctggca gaagtcatca
     2401 cgccggaagg caaaacgcag tatgcgtatg acaaatccgg caggctgacg ggtatcttca
     2461 gcccggacgg cacatcacag cgcaccggct atgacgaacg cgggcgggtg aatgtcacca
     2521 ctcagggccg acgggccatt gaataccact accccgatga acacaccgtt atccgctgta
     2581 tcctgccacc ggaagatgaa cgcgacagac accccgatga atccctgctg aaaaccacgt
     2641 accgctacaa cgccgccgga gaactgacag aggtcattct gccgggggat gagacgctga
     2701 cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg ggttttgcct
     2761 gtgaacaggg ctggaatgca gccggccagc ctgtcagtca gcgcgccgga ttttttccgg
     2821 aggaaaccac atggggcggg ctgctcccct cactggtacg ggagtaccgt tacgacagcg
     2881 cgggcaatgt gtcggctgtc accagccggg aagattacgg acgggaaacc cggcgggagt
     2941 accggctgga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc gggctgggct
     3001 atggcgaagg cgatgagtcc tatggctatg acagttgtgg ctacctgaag gcgcagtctg
     3061 cgggcaggca ccggataagt gaagagactg accagtatgc cggaggccac cggctgaaac
     3121 aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc cgcaccagac
     3181 accgtgacgg ctaccgcccg gaaacagagc ggttccggtg ggacagccgg gaccagctga
     3241 ccgggtattg cagcgcacag ggtgagcagt gggaataccg ccacgacgcc agcggcagac
     3301 gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtacctgtgg gacggcgaca
     3361 gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtacgg cacctggtgt
     3421 ttaacggctt tgagctgata agccagcagt tcagccgggt acggcaggcg catccgtcgg
     3481 tggccccgca gtgggtgacg cggacgaatc atgcggtgag cgacctgacg ggccgcccgc
     3541 tgatgctctt taacagtgaa ggtaaaaccg tctggcggcc gggacagacc agcctgtggg
     3601 ggctggcact cagcctgccc gcagacaccg gctacccgga cccgcgcggg gagtgggacc
     3661 cggaagccga ccccggcctg ctgtatgcgg gacagtggca ggatgcggaa tcggggctgt
     3721 gctataaccg gttccggtac tacgagccgg aaaccggaat gtacctggtg agtgatccgc
     3781 tggggttgca ggggggtgag cagacttacc ggtatgtgcc taatccactg gggtatattg
     3841 atccgctggg gctggcaaaa acttctgttc ccgcagaaaa aataagcctt tcggataaag
     3901 caagagatct ttttagacaa ggaaaagtaa gagaagcatt agatgttcat tatgaggatc
     3961 ttgtacgaag aaagcttggt ggtatatctc aagaaattgc cgggcgtgaa tatgatgttg
     4021 ttactgacaa gatcatagcg caggtcaaga ggacttatag ctctatagat aatccaaaaa
     4081 acttcttaag taagtctact cggacacaaa ttaagaagac aattgagctg gctgaagaac
     4141 aggggaaaga ggctcagttt tggtttaaat atggagtaag ccctaaagta agagaatata
     4201 tagaaagcaa aggtgggaaa gttatattgg gtatggggaa ttaacatgtc aagatatata
     4261 cttattagag gttggtttga gtgcgacttt gaacaagtaa aaaaaataaa agaagctaat
     4321 gaagaatttt tcaaacattc tgaaaaatat ttattaccag aaaacacgaa acgactttat
     4381 ttaaatgggt ggtgttaccc agaatatcct atcaactggg ttagcgttat ttccttaggt
     4441 gttaatataa atcaaattgc at
//
LOCUS       DAZFDP010000264         4428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFDP010000264 DAZFDP010000000
VERSION     DAZFDP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..191)
                     /locus_tag="ACHJ1G_005024"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="ACHJ1G_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            341..1009
                     /locus_tag="ACHJ1G_005025"
     CDS             341..1009
                     /locus_tag="ACHJ1G_005025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4766704.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(954..1091)
                     /locus_tag="ACHJ1G_005026"
     CDS             complement(954..1091)
                     /locus_tag="ACHJ1G_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO4766705.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1064..1648)
                     /locus_tag="ACHJ1G_005027"
     CDS             complement(1064..1648)
                     /locus_tag="ACHJ1G_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4766706.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1648..>4428)
                     /locus_tag="ACHJ1G_005028"
     CDS             complement(1648..>4428)
                     /locus_tag="ACHJ1G_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766707.1"
                     /translation="ILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEALR
                     RFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAGQA
                     ASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSAAT
                     SASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSE
                     TNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSAST
                     ATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSAS
                     ASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDI
                     ASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQK
                     GIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSASE
                     TLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGW
                     FKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRT
                     STGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGL
                     TDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVI
                     PDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNT
                     GAHTHSLSGSTSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGT
                     MANTNSAGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTV
                     KNIAFNYIVRLA"
BASE COUNT          962 a         1167 c         1097 g         1202 t
ORIGIN      
        1 gagatagtaa tgactgttac tgtaacatga gttacaaaca gcttcatacc cagagctact
       61 gatgtatcaa ctcgattcaa caatacccga tatttattcc atgcctccag caacgatctt
      121 tcttcctccg ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg
      181 aattcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgttgtatc
      241 gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt
      301 catcgccata attgatttaa ttcacaaata aaactataac atggtgaaat taatgaaaaa
      361 aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg
      421 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc
      481 acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta
      541 ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct
      601 gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt
      661 ttttgaacct tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct
      721 tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca
      781 ggccttaact gacgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca
      841 caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac
      901 aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac
      961 caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc
     1021 agcacgttct tgcttaagac gtgctgcggc ataatcccaa tgattactcc ctgacagggt
     1081 tcgtaggcca ctcaatatca ggtgcagttg atgtatcaac acggttcagc aacacccgat
     1141 actttttcca ggcttccagc aatgaggttt cttcctccgt tgcgatttcc agatctacag
     1201 catcctgaag tggcgcaata tgctcactgg ctacctgcat caggctgttt tttatttctt
     1261 ccgcctctcg gatccggaac agtttttctg cttctgcatc tttcacccag gctgtgccgt
     1321 tccacttctg aaactcccct tccggcgata accaggtaac attttccggt aacggaccga
     1381 gttcagaaat aaataacgcg tcccctgacg ctacgtcata aaccgtttta ccccgatggt
     1441 cttcaacgag atgccacgat gcctcatcac tgttgaaaac agccacaaag ccagcaggaa
     1501 tatctggtgg tgcaatatcg gtactgtttg ctggcagacc tgtatgaggc ggaatatatg
     1561 catcaccttc accaataaat tcattagttc cggccagcag attataaatt tttatggtcc
     1621 gtgcttgttc actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat
     1681 gtttttgacg gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga
     1741 gccaatcgca acggagtgcg tatgagcacc aataccgaca gtatgtgcgt gtgcacctgc
     1801 gcttgcagca gtgccggaca gtgagtgggt atgcgcgcca gcagagttcg tattcgccat
     1861 cgtgccaatg cccagaccaa ccgccccttg tacctggtat gagttacttc cgacagctgt
     1921 agaaccatac tgataggtgt ctttaaaagt gcttgtatta aacctgcgac catcaacgtg
     1981 cgagtgtgct ccggccgagc ttgtagagcc gctcagactg tgcgtatgag ccccggtgtt
     2041 attcgtggat ttagtgccgt aatcaaacga cgatgtggtt ttcgtcccca aatccgtact
     2101 ggatgcgctg gcgctgtggg tgtgcgattt aatgccgtcc tgttcctgag acaatacggc
     2161 acgaccactg gcgggtttgc ccttaatcgt ccagccacgc atatcaggga tcacgcctga
     2221 cggataagcg gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag
     2281 ggcatagcca gacggaacgg tatctgatgg ccacgggatt ggtgcgccag gcggataaaa
     2341 ctgctctgat ggcgtataga gtgaataaac tgtaccgtcc gttaaccctt ccggcttatt
     2401 agcagaatat gctggtgacg tatgaatcgt cacgctggca ttactggtat aatcccattg
     2461 aatatttaca ccagtcgcat aatttccgat tgcaacgtaa atatcgtaag tatcaccaga
     2521 tgtattgacc caggcaaaat ttgtaaaccc tgtcgatgtg cgctgccata aagcaccagt
     2581 aatccccttc ggattaccat tacctgcacg caaaacaagt tcagatatac ctgcctgttg
     2641 aggtgacccc acgttaaatc ccgcgccacc aatcaacgta attgaaacaa cagaactcgc
     2701 ctgtggcatg gttaccgttg ctaatttgaa ccaaccagca ccaccgctga atgacattgt
     2761 tgttgagtta agcgtaccaa tatctttcgg cgtcagtgtt atatccgctg aaagcgcctt
     2821 accattcacc ttacgggcag aaggtacccg accattcgca ttatcattag ctgctttcac
     2881 tgctttcggt gtctcggcaa gcgtttcaga tgcgctgttg gttgcactac tgagctggac
     2941 aattcctttt tgtgctgtcg tagcgtcctg agcggtatat ttcccgttag caaggtcata
     3001 ggctgccttt accgactttg gcgttgccgc cagcatttca gatgtgctgt tggtcgcgct
     3061 gctgagctga acgatccctt tctgtgctgt cgttgcatcc tgtgcggtgt atttcccgtt
     3121 agccaggtca tacgcggcct taacggcttt tggcgttgcc gccagtgact cggaagtgct
     3181 gttagtcgca ctgctgagct gtactatccc ctttttcgtc gtgctcgcat cctcaagcgc
     3241 cacggcggat gcaatatcct ctgcccgttt agctgctgtc tcggcgcgcg ttgccgcgga
     3301 ttccgccgta cttttgctct gagctgccgc cgtcgcactg ccagctgcct ctgtcgcctt
     3361 cgtggatgcc gtcgtggcgc tgctcttcgc tgctgacgcc tgtctggtcg cctcatcttt
     3421 tgaagcagac gccgatgatg ccgatgacgc cgccgaactg gctgacgatg cggcagccgt
     3481 ttttgaggat tctgcgctgg tttccgacgc tttcgcgttc gtttcggatg tcttcgctgc
     3541 ggaagcagaa ctcgctgctg cgctggcctg ttcagtggct tcgccagcct tcgttgtggc
     3601 tgttgaagcg gatgatgcgg cgctttctgc cgattttccg gcggcggtgg cactggctga
     3661 ggcctgcccg gcacttgttg acgcggcact ggcagacgac gcagccgctg tttttgagcc
     3721 tgccgcagct gaggcgctct gtcccgctgc cgtctcagaa gacttagcgt tcgtctcgga
     3781 cgtttttgcc gccttcgcgg aatttcctgc cgccgttgcc gaggaagcgg cactactggc
     3841 gcttgatgat gcgttcgttt ctgatgattt tgccgcttct tttgaagccg acgcatcccg
     3901 ggctgaggtg gcagcttctg acgctttcgt ggtcgcggtg gatgcagaag tggctgctga
     3961 ttgttgtgac gctgccgcat tcgtttctga cgttttcgcc gcagcggcac tggtggccgc
     4021 cgcgcttttt gaggactctg cagcggcagc actttttgat gcttcagtgg cctttgttga
     4081 tgccgttcct gcgctggaag acgctgactg agccgacgac gcggcctgtc cggctgacgt
     4141 gctggctgcg cgtgctgagc ctgcagcatc agtcgcatgg gttgccgcct cacgggctga
     4201 tgtgctggca tcgctggctg acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc
     4261 gttacgcgcc acctcttcca ccatcagttc aaaacggcgc agtgcctcag gacgggcatc
     4321 atcctccgtc atggcaccga gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac
     4381 ggtgatggtc ccggcatgtg acggcgggaa gccttccacc aacagaat
//
LOCUS       DAZFDP010000265         4413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFDP010000265 DAZFDP010000000
VERSION     DAZFDP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(350..922)
                     /locus_tag="ACHJ1G_005029"
     CDS             complement(350..922)
                     /locus_tag="ACHJ1G_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4766708.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(996..1496)
                     /locus_tag="ACHJ1G_005030"
     CDS             complement(996..1496)
                     /locus_tag="ACHJ1G_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4766709.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(1493..2227)
                     /locus_tag="ACHJ1G_005031"
     CDS             complement(1493..2227)
                     /locus_tag="ACHJ1G_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO4766710.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNNADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACGSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQAAELKARGLLS"
     gene            2779..3045
                     /locus_tag="ACHJ1G_005032"
     CDS             2779..3045
                     /locus_tag="ACHJ1G_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4766711.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            3042..3641
                     /locus_tag="ACHJ1G_005033"
     CDS             3042..3641
                     /locus_tag="ACHJ1G_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4766712.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            3634..3921
                     /locus_tag="ACHJ1G_005034"
     CDS             3634..3921
                     /locus_tag="ACHJ1G_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO4766713.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            3914..4369
                     /locus_tag="ACHJ1G_005035"
     CDS             3914..4369
                     /locus_tag="ACHJ1G_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766714.1"
                     /translation="MFDFPRPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQEPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
BASE COUNT          960 a         1206 c         1115 g         1132 t
ORIGIN      
        1 tcacctgaag atcgcttgct aaatatatag ttccaatatt caagccgcta tagtagcggc
       61 tttttacaca aaatgcttta cccaacaaac attacgttac aactattaat taccaatata
      121 ataatacagt tatctgtctt tgattataaa acaccacttg cgcgcagtgc tttccccgcc
      181 tcgcccgccc gctttgcggg gcggttttaa tgcagtttca ctgacacgct caggccgcgc
      241 cgggaatggc gcggtctgca gaaaatgagg caggaaaacg catgcaaagc catgcacctt
      301 atcgatgcat ggcttttttc agtaaaaaca ggcggatttt cggggaattt tacacagact
      361 gacgtgatgc cagttgcgca cttttacgcg aaaaaatcat gttctgcgca ggggtgaatt
      421 tttcacggct gtcatccacc gaagccgcgt caggcctgaa tccgatggtc gttaaaatgt
      481 cgctatcctg tgcggaataa ttaatttttt cccccttcgc aagccaggac aggagggctt
      541 cacgcagggc atctgtggca cgctgtatgg cacagtttcg ggcaatggcc gtcagctcac
      601 tgtagcccat cagctccggt gccagtgccg ccgccagtgc tgtgccgtgc tgctgcataa
      661 aatcattcag ccggtcgcgg atgctgatgt gctgaacggc ttcatgcgaa cgaatataac
      721 gaccagcggc ctgattcacc tgccattttc tgacttcgat aatattgcgt aattcgtcca
      781 ggcgactgac gtttctgcct tctcctgaca gaagccgcag atattcctgt tcggccgctg
      841 ccagctcatt tttgcgttgc agccatgctg ctttgttatt ctgacaggtg tcaaaggcct
      901 gctgtaaggc tgtgctttcc atcgttatct ctttctcatc atgctgaaga ataaaaatac
      961 ggtgtgcggc gacggccggt gttaaccggc agccctcatt ccagacgcag cgaatatgat
     1021 tgtgttttta agcgtactgg cggcagttcc tgtttttcat gcaggctttc tgccagttcg
     1081 tccggcgtga ccgggcggac aatgaaacgg ttgatggtct gaagcgtttt aaacaccaga
     1141 ccacagcccg gatccgtgca cacataaaaa cgctcggtga cttcctgaga cagacgccgc
     1201 gatgttcttg acagtgcaag gcctttacat ctccgacaac aatatccggt aacaagcatt
     1261 cttttcggcc gtttcatact gccggaggct gacgtcagtg aatcgcggta tctctgtttg
     1321 cctgaaatgt attccattcc tgaatcttta cagtcagaga aaaagctttc actcgcttca
     1381 aatgtcgcag agcaataaat attccggcac tgtgcaatca ttatcttggt gccatcgtcc
     1441 atgaaatgtg cgcgacgggt gtgagcaaca tgtccacacg acgggcagta aatcatgaca
     1501 gcagccctct ggccttaagc tctgctgcct gctggtctat tttgtcctgc cacaccttgc
     1561 gctgtgccgg tgtgcctgcc acctcataat ccatgtgcgg aagtgttgcc gctgacagtc
     1621 cggtcagccg gagaaccggc tcgccggtga ggctgatttg catctgttta attttctgtt
     1681 ccagcgatga tttcacctgc tgcatgacag ccttttccgg tgcgacgtag ccctgatggc
     1741 cggtggtgtt ggcgagcgga ttttcctgta ccaggatact cagatgcatt gcccggacaa
     1801 gcgcctcaca ggtttcattc agggcgtgtt ccagctcatg ctctgcatac agactcagaa
     1861 ggtgatgatg tgccttccgg taggcggtgg ccgtgctgcc acacgcccct ttcaggcgtt
     1921 cacgttcgaa attcagcacc acggccagat tgtcatattc ctgtaccagc tcccggcgtg
     1981 ccacgcgctc aatgtggcgc tgtttcagct cgtcgctcag gacaccaccg gctgcacgaa
     2041 aggccgtgcg ccagtcgtca gcgttgtttc cgtcggcctg cgccagcgca tttttttcct
     2101 gctctgcccg ttcaatggcc gtgacggtct catccatcag gcgggcgttc tcaagatggg
     2161 cggctctggc cttttccagt tgtgccagcg cgggttgcag atattcaggg atggtgttgt
     2221 cagacatttt ccggctcctc gtcacttcag gttaagaaaa ttgtgacgta caccggacaa
     2281 caacacgacg cattgcagat gtgccagccc tgacacagga gactcatcct cagaccggca
     2341 agccaggaaa aggtcgcagg aaaaaccggc ttactgtttg tttttttata ttttactgtt
     2401 cacctctgtt caccataaga aaaaagataa gtaatacagt aagttaaagg gtgaacaatc
     2461 gcagtaatga ctgttcaccg tctgttcacc actgttcacc cgttgatggg ctttttgtgc
     2521 tgtttattac tttttatttt tattaattca ctgaaataaa taagaaaaaa caatttgtat
     2581 ttcactataa ttttttccag ctccttccag agcgttttaa agctatctgg cgctgatgta
     2641 caaaaaatac acagccattg taaggctgcc ggaacaaatt ccccctgttg cgtctgccga
     2701 aaatattcac aaaataaagc gctacccgaa gccggacgga cttatccggt gctgtatgga
     2761 cattaacgag gtagcccgat gcaagctgtt ttttcttccc cgtctcccgc cccagtgacg
     2821 ccactgatgc cgctgccgga catcacgcag gagcgttttt tacgtctgcc ggaagtgatg
     2881 cacctgtgcg gcctgtcacg ctcgaccatc tatgaactta tccgtaaggg ggaatttccg
     2941 ccgcaggtga gtcttggcgg taaaaatgtg gcctggctgc actctgaagt caccgcatgg
     3001 atggccgggc gcattgccgg acgcaaacgg gggtacgacg catgatgatg cccgctctgc
     3061 aaaaactccc tttttctggc ttgccttttt ccggcatttg cggatatagt ttttccgctg
     3121 ccgcaaaatc ggcagccggg cgtgagaacc cgagcaattc acaggcgaca tatgacgcgc
     3181 cacgcgtctt tttttgtgtc gcaatcaatg ccacagagcg ccagattatg gtgtggtgtg
     3241 tggtcagtcg tgcagatggc attccgtgca tcctgcccgc gtccgctcat tatgccgcag
     3301 agtcaatggt agctcaggcg gggcagcctc cgggctggcc ggtatcctgt gaagccggta
     3361 ttctcacccc cgtctgggct atcgccatcg agcgtgagaa ctccggcgat agcgttattt
     3421 gctattcaca ggaggctgcc atcatggcta caacccttac cccctcacac cctgaatttg
     3481 tctttgtgtt tgcggctgtc cgtcgcgcag accgtcatcc ccgtatctgt atgcttcgca
     3541 ccgtcgccgg tgatgaacgc agtgcccgcc gttcccttgt ccgtgactat gtgctctccc
     3601 ttgctgcccg tctgccggtg gtggaggtgt cccgtgcgta ataaaaaagc ccctcagacc
     3661 gtctcagcac gtcatgacgc ccgtgaacac ctcagcattg aggcttacca taagctcaac
     3721 cgcgccagcg ccgtatccca gtttgttggg ggtgatttga ttcaccgtga actctccggc
     3781 ctgcatcagc tctacattcc gcatattttc agctacctga atgaagatat tgattttgtg
     3841 ctgaatgagc tgaaagccaa aggcctgtgc cgcgattttc tcgcccagca gaaagaccgg
     3901 ggagacagga cgcatgtttg attttccccg gcccggtgag atttaccgtt ctgccggttt
     3961 tcccgatgtg gccgtggtcg gcattctgga agacggtatt ccgtgggaaa tgccgtaccg
     4021 ctgcccggaa attgtctgga acccgtaccg ccgtaaattc agtatccttg tgcgtatcct
     4081 cgctgacggg cgcaccacag acatcccgct ggggcgtttt ctgcgggaat ttacctgtga
     4141 ccgtcctgac ctgtttaaac gcagccccgt aaaccggcat gcggtactga aagaaatggc
     4201 cggagacccg gaattacaga aatggcggga gaaatatctg gatatttacc cgcaggagcc
     4261 tgttccggtc agccgggcgg caccggtggc gcgggaatgg cgggaaattc cccgcacgga
     4321 gcctgacccg gaaatcaccc cggataacag ttaccgcaat tatctgtaat taaaaaacga
     4381 cacccgaaaa aaaaattaaa tgtgcgtatt cgc
//
LOCUS       DAZFDP010000266         4412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFDP010000266 DAZFDP010000000
VERSION     DAZFDP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..77)
                     /locus_tag="ACHJ1G_005036"
     CDS             complement(<1..77)
                     /locus_tag="ACHJ1G_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4766715.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAA"
     gene            complement(366..731)
                     /locus_tag="ACHJ1G_005037"
     CDS             complement(366..731)
                     /locus_tag="ACHJ1G_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4766716.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            773..958
                     /locus_tag="ACHJ1G_005038"
     CDS             773..958
                     /locus_tag="ACHJ1G_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4766717.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(1088..1228)
                     /locus_tag="ACHJ1G_005039"
     CDS             complement(1088..1228)
                     /locus_tag="ACHJ1G_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766718.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1585..1809)
                     /locus_tag="ACHJ1G_005040"
     CDS             complement(1585..1809)
                     /locus_tag="ACHJ1G_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766719.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1874..2080)
                     /locus_tag="ACHJ1G_005041"
     CDS             complement(1874..2080)
                     /locus_tag="ACHJ1G_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4766720.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(2308..2454)
                     /locus_tag="ACHJ1G_005042"
     CDS             complement(2308..2454)
                     /locus_tag="ACHJ1G_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766721.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            complement(2454..3020)
                     /locus_tag="ACHJ1G_005043"
     CDS             complement(2454..3020)
                     /locus_tag="ACHJ1G_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4766722.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMIDFVLADTGKGKITKAD"
     gene            complement(3291..3824)
                     /locus_tag="ACHJ1G_005044"
     CDS             complement(3291..3824)
                     /locus_tag="ACHJ1G_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766723.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(3875..4219)
                     /locus_tag="ACHJ1G_005045"
     CDS             complement(3875..4219)
                     /locus_tag="ACHJ1G_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4766724.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            complement(4224..>4412)
                     /locus_tag="ACHJ1G_005046"
     CDS             complement(4224..>4412)
                     /locus_tag="ACHJ1G_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4766725.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKTARGD"
BASE COUNT         1139 a         1202 c          953 g         1118 t
ORIGIN      
        1 gcggcaatgg ctgacgctgt tttgtttttc ccggacatga gcgccagcgc aaattttcgt
       61 ttttgcgttg tcagcacagc ctcctcccgg gtccagaacg cactcagccg ggtatggttc
      121 agcccatttt tcccggcgtc tcatgccgca aatgttaact gctgcctggt taacatttgc
      181 tgaaaaagcc tgttaacatt ttttccgcac aacaaactga ataataaaga taaaaaccgc
      241 aaaaatgccc ggacagccag ttaacatgtt aactgccctg aaacgggaat tttttctctg
      301 cgtgagaggg ggcgcggtgt ccggagcgat cgttttttac gccggatgat acccccccgc
      361 tcgggttaca gtccgatgat gtcgtccgct ctgccactac ctccggacac ctccggcagc
      421 gtcgggtccg gcataccacc cgccgcttca cgagcagact tttgtcgatg gcattcggta
      481 cagagcgtcc agagattcgt ctcctcatta ccaccaccga actgaagtgc aattcggtga
      541 tcgagttcac tgtcacagag gtcaaccaca cgaccacaga gacagcactg cccggcatcc
      601 ctgagccaga tatgacgctt gagggaaaca cgtgcactgc cactgacacg acgctgttca
      661 cccttcagaa tattcacccg tcgggtattc agtgttttga ttctgctctg gagtgtacga
      721 agctcagcca tgtaaaatcc ccgtcatatg gcaatcagta aaggaaataa atatgtcatc
      781 gaaaaaccgg acccgcagaa ccacaacccg caatatccgt ttccccaatc acatgattga
      841 acagatcaat atcgcccttg aacataaagg ttccggtaac ttttcagcgt gggttattga
      901 agcctgcaga agaaggctgt caacagagcg ttcgggtatg aattacataa ttaagtaaca
      961 tggtgttcac agaacacaca gttaccggac acatcagttt tccattcgtc ccccggcagt
     1021 acaggcttcc cgtctgacgg gatagcctga aaaaacacag aaaattattt gttataatta
     1081 atataactta ctcaaaaaaa agcgacgaga aaatcagcat caacgagcaa taagcgccaa
     1141 tacgtgataa caaatggcag ccatatttat ctgcagtata agcaatggac aggataacca
     1201 caccagaaac cgtcagcata aaatccattt gaacttcccc ggacaaaatc gactcatcta
     1261 aagatttaca gctcttttta ttatcaatat gttaaaagta aaataaacag atgttcaata
     1321 atacgaatac aaaaacgtgc tgaaaatcaa tgaatccatt tctgtgttat caattaatag
     1381 tgataaacat ccggcttctt ccaccatcgc accggaccag cgaccatgag gggacaacgc
     1441 cgcgctccgt taacgcggta aaccccggtg tgtatcgttt ttgattatcc ccgcacactc
     1501 gcgcagagga ttctcccggt cgggctgcgg tctctgttaa tgcaggaata cggcgacaat
     1561 accgcgcatg aataataagg tcgctcaaca cactggctgt aatgcagcgg ataccatgcg
     1621 gcatttagcg gcattcatcg tacactcaac ggttagctct tcattcgtgg cattcacctg
     1681 aaaggtcctg gagtgtaatt gcgtacattt accactgaac gaaccttcaa caagaacacg
     1741 accacgctgc aaaatacgga acggaattgt tccctgaaaa ggctttacgg ttaccaataa
     1801 tttcttcatg cattctccga ataacaaaaa tactagttaa tacactgagt gcggatatat
     1861 tcctgaagca ttctcaatgc tgcctggtcg ctgatgattc cgtctctgat accgagaacg
     1921 tttcgtccag caaccggaga gagttcgacg gcggcatcat tgcccacgcc ggaggtgccg
     1981 gtggcttcac gcacggtacc ggggcaggtg gcgttgatcc gcaggcgctt acgaccagcg
     2041 gcaacatcag cacgcagagt ttcattttca gctctcgcat cggctaattc cctcgagtat
     2101 tttgcatcga gcgcagcaac atcacgctgg cgcacctgca tgtcagtaat ggtggcattc
     2161 gcctgttcca gctctctggc ttttttatcg cgctgcgctt tgtaggtgag cgcgttgtca
     2221 cggtaatggt ttgttgccag ccacagcgca ccacagccaa ccgccatgac aataatcacc
     2281 acacacagaa cacggttcat ctctctttca ccccaccagt cccgataacg tcaggactcg
     2341 ccaggcggtg gaaaagaaaa tggcaaccag catgactaaa aatgaaatgc cgacaagtac
     2401 acagaggctc ttcaccagcg ttatgagttt atctgatatc attagccacc ccatcaatcc
     2461 gcctttgtta ttttcccttt gcctgtatca gccaggacaa aatcaatcat attcgcttca
     2521 tttaccagcg tacggatttt tgatacatgc gcggctttaa cctgtttcca ctcattcagc
     2581 ccggtagcaa acacactggc aatgttttta tcccgtttca tgtcagcaca agcctggttg
     2641 agttcttcca tcacgctcat tttacgggga ttaacgacaa aacccttcgt ccagtactcg
     2701 taaagaacat cgtcgcactc ttcctgatac cggatgacct tatcgcggat ttcgggtttt
     2761 actttgttgg gattaatggt ttgtagccag ccggcaagtt ttcgaagtgg catggacacc
     2821 atattgcgtt gtttcccatc ctcagcaacc ataacgattt ccgttatagt tgacgcaaaa
     2881 cgctgtctta acttagccaa ctgtgattgc caggccagcc ccatccccgc aacgacaggt
     2941 ttcatgggaa cgtatggttc gccattatgg ttaactacat aaagagagtt gccgtgaaac
     3001 ggcacggcca tcatattcat cggttatttc cttttagtga tgaaccttgt ctcacaggaa
     3061 tccagcccac agaaaggcac cgacagccaa accggtatcc tcaagggtca tcctgaaagg
     3121 ttctgtgttg tgagatgcgc gtgagatgcg cagaaatgac aaaggcatca ttacggtgcc
     3181 tgagtgttaa acaactgttt tgactttatt cacttacatt ttgccaattt gcaggatttc
     3241 gtgttatcca tccatgtaag caaacctcat ttttcagcaa aatattctgc ttatctgtcg
     3301 attccccagc acgccagcgc gctctcctgg tcacgacggg atacctgacc gtaacagtta
     3361 tttgagcgaa tacggcagtc cctgccaccg tccttaatcc accagcgaat agcttcgcag
     3421 gcaccttttc gatctcctgc attaatccgt ctgtaaaacg tcgacgggaa acacttaccg
     3481 gggccaatgt tgtacggaca gaatgacgca atccccgctt tctggggttc ggtcagtggc
     3541 actctgatgt ttttctccac ccatgccagc gccttatcac gctcaatggc gttaacccgg
     3601 tcgcattttt ccttcgacag cttcatgcca ggaataacag gcttaccatc cacccgggtg
     3661 gctccacggc agatggtcca gatacccgca ccatcacggt atgctgtggt gtggttacct
     3721 tccttttcat ccagaaactg gtcgaggatt tcaggcgcag gcgcacctgc ggcaatcagc
     3781 gccagaacgg cagccgacag gccgtatctg atttttgcgt tcatggatat ttatcagggt
     3841 ttatcgattt caaatccctg gatatgttaa gtcttcaggc cagcggtgga gtcttcagag
     3901 aacccgtaat tattcccggt agttttcctc tgtaggttat caacacatcc tgcgcctcta
     3961 aaattacggg acgcttttcc ggtaacggac catccccttc acataacccg gcagcaacat
     4021 ccatgaaaaa ctgcttcgcc tgctttttcg cctcagcttc gtaaaactcc agcgtggcac
     4081 cttcagtacg gtcaagacta atcgccacat gtggcaacaa caacgacgga tgcccgccaa
     4141 tttcaagtgc cacagtaaca gcaatcttat ccgggtaatt atttatctct ttaacaacca
     4201 gttcgtattt tttcttcatc gctttagtct ccccgcgccg tcttacggcg gtcctccctg
     4261 attttgaaat acaggttagt cagatacgtc agcaggccaa acagcagact ccccagtaca
     4321 cctattgcca cccactggga cggagagact ttgtccagca gttgcagtaa ccagtatccc
     4381 gtccctaccg ctgacgtggt gtatgacaca cc
//
LOCUS       DAZFDP010000267         4398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFDP010000267 DAZFDP010000000
VERSION     DAZFDP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..459)
                     /locus_tag="ACHJ1G_005047"
     CDS             complement(<1..459)
                     /locus_tag="ACHJ1G_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766726.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPS"
     gene            complement(449..628)
                     /locus_tag="ACHJ1G_005048"
     CDS             complement(449..628)
                     /locus_tag="ACHJ1G_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766727.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(804..1361)
                     /gene="ymfL"
                     /locus_tag="ACHJ1G_005049"
     CDS             complement(804..1361)
                     /gene="ymfL"
                     /locus_tag="ACHJ1G_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4766728.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(1405..1605)
                     /locus_tag="ACHJ1G_005050"
     CDS             complement(1405..1605)
                     /locus_tag="ACHJ1G_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO4766729.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1696..2370
                     /locus_tag="ACHJ1G_005051"
     CDS             1696..2370
                     /locus_tag="ACHJ1G_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4766730.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            3039..3401
                     /gene="yfdP"
                     /locus_tag="ACHJ1G_005052"
     CDS             3039..3401
                     /gene="yfdP"
                     /locus_tag="ACHJ1G_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO4766731.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            3467..4291
                     /locus_tag="ACHJ1G_005053"
     CDS             3467..4291
                     /locus_tag="ACHJ1G_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO4766732.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAIRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         1170 a         1072 c         1022 g         1134 t
ORIGIN      
        1 agaaggtttt ttatctgacg gatcatgttt tgattttact gacggatccc cgccagattc
       61 tgacgggtga aaacccgctt ttttgccaga tttcgacgca tcaaattttg acgggtcaga
      121 ttttgatgcg tcagattttg acgggtcaga atctgacagt tgagaaaatg ccgctgcctg
      181 aagcttcgca acgttaagct gataaacatt cgacgcattg cggttaccct ggcgacgcgc
      241 cttacgcgtt aaccagcctt ctgcttccag ccgtgcgata gccgttctga cggtactcat
      301 ccccgcgcca atctgacggg caatggtttc aattgatggc cagcacacac cttcgtcatt
      361 actgaaatca gccaggcggg ccataattgc cacgctggat aatttcatgc ctgacgctgc
      421 gcaaccatcc catacatagc cggttaattt agtgctcatg accgacctct atttccctga
      481 atttacgacg aaactgttcg agcgggctga agcactcatg ctcatagcct tcacggaggt
      541 agataacccg ttgtgtttcc ggctcccaac gaatgactct gacgggcact ccgtagtgat
      601 ctttgaacca gcggttaact tgtcgcaaag gactgtctcc ttctgccggt tgaaatcccc
      661 cacagcccac tctgcaaagc tgtgggttac aatttccctg tcacctggta catttactgc
      721 atagcaatac tccaccttcg cttttccacc cggtacagga agcgcaatca gttgcgagcg
      781 acggtagtgt gttgttaaac tgttcatgcg ttagtttctc cacaaccaga agcaatcgac
      841 gccacgacgc ccggagctgc acactcgcgg gcgtcattac tttctgaaac gcaaaaaatt
      901 ttgtagacaa gtgctgcatg ctcctgcagc ttcgaaattg agaggtacag ctcgtcgtta
      961 attgctgtct tctcatgcgg ttccactaca ccgtcttcga ttgccgaacg aatctgtttt
     1021 gaataactgc cgatctgttc aatgacttcc agtaaacgct ggttaatatc ggcattgtcc
     1081 acatcctcga cgtcaggaag agacacaaag acgccatttg cagactgcgc cacagcgtca
     1141 gcaatgaagt gagtgccacc agcacgttgt aaaatcattg cccatcccag cgggaaaatc
     1201 tgatcgccat cggcacgaag gcggttaaat aatgcgttct ctgttacatc cagccactca
     1261 gcagcttcag cgtaaccccc cggcaacgct gcgatagttt ttctgacagc tttcacgtac
     1321 cactcaggct gtttttctac tttccagtga tacttaccca cggttagcct catcgttctg
     1381 tggttaaaaa ttgaaggtgt tctgttaatc tttcggatag atatccggtc ttaagtcaga
     1441 tttcgtaatt gcacctgacg tgcattgctc aagttttttc gccagcacaa aactggcttt
     1501 tttataacca ttgaaaacca gccgtaagta gccaggtgtt gagccaactt ttccggccaa
     1561 ctcgccctgc tgttctttgg ttaaagagtc ccaatacgct ttcatacaac atgtacctcc
     1621 ggtatacata ttacatgatt gaaatgaacc ttcaagatac ttgtacctta tcggtacaaa
     1681 ggttttaatt tcgttatgaa aacaatccat gacatccggc ggtctaacgc cagaaaactg
     1741 agagatggtg ttggcggaaa ttcatccttt gccaccatga ttgatcgcga gccaacccaa
     1801 accagcaggt ttatggggga tggcgctact aaaaatatcg gtgacagcat ggcacgccac
     1861 atcgaaaaat gtttcgacct gcctgtcgga tggcttgatc aagaacacca gacaacgaac
     1921 atcacaaaaa aacctgatgt ttcaatcact aacaaacaaa taacgttagt ccctgtcata
     1981 tcatgggtac aggccggagc atggaaagaa gttggctatt ctgaggttga tttgagcaca
     2041 gcagaaacgt atccctgccc tgtaccctgt ggcgaaatga cttatatctt gcgggtgatt
     2101 ggtgattcaa tgattgatga gtaccgccct ggagacatga tttttgttga tcccgaagtc
     2161 cctgcctgcc acggtgacga cgttattgca ttgatgcacg atacaggcga aaccaccttc
     2221 aagcgattga tagaagatgg aacacagcgt tatctcaaag cattaaaccc aaactggcct
     2281 gagccttaca ttaagattaa cggtaattgc tctataattg gtacagtgat tttctcggga
     2341 aaaccaagaa gatacacaat aaaggcctaa tcaatattta tgaacctgct tcggcaggtt
     2401 tttttatact tgacaatgta cccatgagat acataatgta tccaaaagaa acatgaggca
     2461 ggcaagattc aaacaaaatt tggttgtaac acggcgtatg gcacatgcgt cgttagcggt
     2521 ctggggacgt taaaggggac aatccactcc ttgctagggc aaacaaacca ggtagccgga
     2581 atgtgcaagt caatgatgat gctgataaga cgcctaacca gcgtggcgat ccggtttgac
     2641 gcctgggaag agaccagggt gcaacgatga gggcatttat ggagccgcga caaagtgtgg
     2701 tgccgtaact ggctaagtgc tctcagcgtt gtggtcatcc gcgaaatggc gcggcggtaa
     2761 gtatggcggg gttactcttt ccccgttgag gacaccggat tgtcaggttg accatacgcc
     2821 tgagtgacaa ccccaccaca acagccactg ctttggcggt accagtttgt acacttgctt
     2881 ccggctggta ccgctctttt tacaaaacag agaagagcat caccggacga cgggctcata
     2941 acccaatcca cccgggcggc tgccaccgca ggtgctcttc tctgttttgt ggagaaacca
     3001 accgaccttg cagggtcgat atgatgagga gcagcaaaat ggctagcgaa cgcagtactg
     3061 atgtgcaggc atttatcggg gagctggacg gcggcgtatt tgaaaccaaa atcggcgctg
     3121 ttctcagtga agtcgcttca ggtgtgatga acacgaaaac caaagggaag gtctcgctca
     3181 acctggaaat cgaaccgttt gatgagaacc gtgtgaaaat aaaacacaaa ctctcatatg
     3241 ttcgtccgac taaccgcggg aaaatttccg aagaagacac caccgaaacg ccgatgtatg
     3301 tcaatcgcgg tggtcgcctg actattctgc aggaagacca gggacaatta ctgactcttg
     3361 ccggtgaacc tgacggaaaa ctccgcgcag caggtcatta atatcgttct taattaactg
     3421 attatttatc tcatcactga atatctttat atagtgagga cttattatgt ctcagaactt
     3481 agacgcaacc gcaattaatc aaatccatgc ccttatttct gctcagggtg ttaatgaaat
     3541 tattagtaag attggtgccg atgctgtggc attgcctgag aatttccgca ttcatgatct
     3601 ggaaaaattt aatttaaatc gcttccgttt ccgtggtgcg ctttccactg ccagcatcga
     3661 tgactttacc cgttattcta aagatcttgc agatgaaggc acccgctgct ttatcgatgc
     3721 cgataatatg cgtgccgtca gtgtgcttaa cctgggtact attgatgaac caggtcacgc
     3781 agataacacc gccactctca aactgaaaaa gacagcaccg ttctctgctc tgttgtctgt
     3841 taatggcgag cgtaactccc aaaagtcact ggcagaatgg attgaagact gggccgacta
     3901 ccttgtgggc tttgatgcta atggtgacgc cattcaggca accaaagcgg ctgcggcgat
     3961 ccgtaaaatc acaattgaag cgaaccagac cgctgatttt gaagacaatg acttcagcgg
     4021 caaacgctcc ctgatggagt ctgtcgaagc gaagaccaaa gacattatgc cagtggcatt
     4081 tgaatttaaa tgcgttccgt ttgaaggcct gaaagaacgt ccgtttaaat tacgcctcag
     4141 cattatcact ggcgatcgtc ctgtactggt tctgcgcatt attcagctgg aagcggtgca
     4201 ggaagaaatg gctaacgaat ttcgtgatct gcttgttgag aaatttaaag acagcaaagt
     4261 agaaaccttt attggtactt tcaccgcctg atttcattac tgcaaatgcc cctgcggggg
     4321 catttatgga aacgtaatta actcaataat caccggatgg tgagagcttc cttttagcag
     4381 aattcagcgc ggtgcagc
//
LOCUS       DAZFDP010000268         4332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFDP010000268 DAZFDP010000000
VERSION     DAZFDP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..553
                     /gene="fimA"
                     /locus_tag="ACHJ1G_005054"
     CDS             11..553
                     /gene="fimA"
                     /locus_tag="ACHJ1G_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4766733.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            773..1465
                     /gene="fimC"
                     /locus_tag="ACHJ1G_005055"
     CDS             773..1465
                     /gene="fimC"
                     /locus_tag="ACHJ1G_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4766734.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1496..4105
                     /gene="sfmD"
                     /locus_tag="ACHJ1G_005056"
     CDS             1496..4105
                     /gene="sfmD"
                     /locus_tag="ACHJ1G_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4766735.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFEGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4147..>4332
                     /locus_tag="ACHJ1G_005057"
     CDS             4147..>4332
                     /locus_tag="ACHJ1G_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766736.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPG"
BASE COUNT         1162 a          945 c         1018 g         1207 t
ORIGIN      
        1 aggaatatat atgaaattaa gatttatttc gtctgcgctg gctgccgcac tattcgccgc
       61 tacgggtagt tatgctgccg ttgtagatgg cggtacaatt cactttgaag gcgaactggt
      121 gaatgctgcc tgttcagtga atactgactc ggcagaccag gttgtcacac tcggtcaata
      181 tcgtaccgat attttcaatg ctgttggtaa tacctctgca ttaattccat tcaccattca
      241 gttgaacgac tgcgatcctg ttgttgccgc taatgctgcc gttgcatttt ctggtcaggc
      301 tgatgcaatc aatgataatt tattggccat tgcatccagt accaatacaa caacagcaac
      361 gggtgtcggt attgaaatac ttgataatac atccgcaatt ctcaaacctg atgggaatag
      421 cttctcaacc aaccagaact tgatccccgg gaccaacgtt cttcattttt ctgcacgtta
      481 taaaggcacc ggtacaagtg catcagcagg gcaagcaaat gctgacgcga cttttattat
      541 gagatacgaa taatcaaaac cacgttgttt tgaattatat atcacgtctt ataacaaagt
      601 aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa tcgaaatcat gttcactttg
      661 tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt gtaagaaacc aaagcaatca
      721 tttctttata tttcttattt ttgccgtcag gaatacacaa ggcgtattaa ctatgatgac
      781 taaaataaag ttattgatgc tcattatatt ttatttaatc atttcggcca gcgcccatgc
      841 tgccggaggg atcgcattag gtgccacgcg tattatttat cccgctgatg ctaaacagac
      901 tgcggtatgg attagaaata gccataccaa tgagcgcttt ctggtcaatt cgtggattga
      961 aaacagcagc ggtgtaaaag aaaagtcatt catcattaca ccgccactgt ttgttagtga
     1021 acccaaaagc gaaaatacct tgcgtattat ttacaccggt ccaccgctgg cagcagatcg
     1081 tgagtctctg ttctggatga atgttaagac gatcccttcg gtagataaaa atgcattgaa
     1141 cggcaggaat gttttgcaac tggcgatttt atcgcgcatg aaattatttc tccgtctaat
     1201 tcaattacaa gaattacccg cagaagcacc ggacacactc aagttttcgc gatccggtaa
     1261 ctatatcaat gttcataatc cctcaccttt ttatgtcact ctggttaact tacaagtggg
     1321 cagccaaaag ttggggaatg ctatggctgc acccagagtt aattcacaaa ttcccttacc
     1381 ctcaggagtg cagggaaagc tgaaatttca gaccgttaat gattatggtt cagtaacccc
     1441 ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca atatcagagc taattatgaa
     1501 aatacccact actacggata ttccgcagag gtatacctgg tgtctggccg gaatttgtta
     1561 ttcatctctt gccattttac cctccttttt aagctatgcg gaaagttatt tcaacccggc
     1621 atttttatta gagaatggca catccgttgc tgatttatcg cgctttgaga gaggtaatca
     1681 tcaacctgcg ggcgtgtatc gggtagatct ctggcgtaat gatgagttca ttggttcaca
     1741 ggatatcgta tttgaatcga caacagaaaa tacaggtgat aaatcaggtg ggttaatgcc
     1801 ctgttttaac caggtactcc ttgaacgaat tagccttaat agcagtgcat ttcccgagtt
     1861 agcccagcag caaaacaata aatgcatcaa tttactgaaa gctgtacctg atgccacaat
     1921 taactttgat tttgcagcga tgcgcctgaa catcactatt cctcagatag cgttgttgag
     1981 tagcgctcac ggttacattc cgcctgaaga gtgggatgaa ggtattcctg ctttactcct
     2041 gaattataat ttcaccggta acagaggtaa tggtaacgat agctattttt ttagtgagct
     2101 cagcgggatt aatattggcc cgtggcgttt acgcaacaat ggttcctgga actattttcg
     2161 cggaaatgga tatcattcag aacagtggaa taatattggc acctgggtac agcgcgccat
     2221 tattccgctg aaaagtgaac tggtaatggg agacggcaat acaggaagtg atattttcga
     2281 gggtgttgga tttcgtggta tacggcttta ctcttccgat aatatgtatc ctgatagcca
     2341 gcaagggttt gccccaacag tacgtgggat tgcccgtacg gcggcccagc taacgattcg
     2401 gcaaaatggt tttattatct atcaaagcta tgtttccccc ggcgcttttg aaattacaga
     2461 tttgcacccg acatcttcaa atggcgatct ggacgtcacc atcgacgagc gcgatggtaa
     2521 tcagcagaat tacacaattc cgtattcaac agtgccaatt ttacaacgcg aagggcgttt
     2581 caaatttgac ctgacggcgg gcgattttcg tagcggtaat agtcagcaat catcgccttt
     2641 cttttttcag ggtacggcac tcggcggttt accacaggaa tttactgcct acggcgggac
     2701 gcaattatct gccaattaca ccgccttttt attagggctg gggcgcaacc tcgggaactg
     2761 gggcacagtg tcgctggatg tgacccatgc gcgcagtcag ttagccgacg acagtcgtca
     2821 tgagggggat tccattcgct tcctctatgc gaaatcgatg aacaccttcg gcaccaattt
     2881 tcagttaatg ggttaccgct attcgacaca aggtttttat acccttgatg atgttgcgta
     2941 tcgtcgaatg gaggggtacg aatatgatta cgattacgac ggtgagcatc gcgatgaacc
     3001 gataatcgtg aattaccaca atttacgctt tagccgtaaa gaccgtttgc agttaaatat
     3061 ttcacaatca cttaatgact ttggctcgct ttatatctct ggtacccatc aaaaatactg
     3121 gaatacttcg gattcagata cctggtatca ggtggggtat accagcagct gggttggcat
     3181 cagttattca ctctcatttt cgtggaatga atctgtaggg atccccgata acgaacgtat
     3241 tgtcggactt aatgtttcag tgcctttcaa tgttctgacc aaacgtcgct acacccggga
     3301 aaatgcgctc gaccgcgctt atgcctcctt taacgccaac cgtaacagca acgggcaaaa
     3361 tagctggctg gcaggtgtag gtgggacctt actggaaggc cacaacctga gttatcacgt
     3421 aagccagggt gatacctcga ataatgggta tacgggcagc gccacggcaa actggcaggc
     3481 cgcttacggt acgctggggg tcgggtataa ctacgaccgc gatcaacatg acgttaactg
     3541 gcagctgtct ggcggtgtgg tcgggcatga aaatggcata acgctgagcc agcctttagg
     3601 ggataccaat gttttgatta aagcgcctgg cgcaggcggt gtacgcattg aaaatcaaac
     3661 tggcatttta accgactggc gcggctatgc ggtgatgccg tatgccacgg tttatcggta
     3721 taaccgtatc gcgcttgata ccaatacgat ggggaattcc atcgatgttg aaaaaaatat
     3781 tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc aattttgata cccgcatagg
     3841 cgtgcgggcg ctcattaccg ttacccaggg cagaaaaccg gtgccgtttg gatcactggt
     3901 acgggaaaac agtaccggaa taaccagtat ggtgggtgat gacgggcaag tttatttaag
     3961 tggtgcgcca ttgtctggtg aattactggt tcagtgggga gacggcgcga actcacgctg
     4021 cattgcgcac tatgtattgc cgaagcaaag cttacagcaa gccgtcactg ttatttcggc
     4081 agtttgcaca catcctggct cataaaggaa attatcaata agataatctg cagattatta
     4141 ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg tgcaaatagt
     4201 actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag caccaataac
     4261 cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt ctcagcaatt
     4321 tgtccacccg gt
//
LOCUS       DAZFDP010000269         4304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFDP010000269 DAZFDP010000000
VERSION     DAZFDP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3826
                     /gene="espP"
                     /locus_tag="ACHJ1G_005058"
     CDS             <1..3826
                     /gene="espP"
                     /locus_tag="ACHJ1G_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO4766737.1"
                     /translation="SSRATGKKKHKRILALCFLGLLQSSYSFASQMDISNFYIRDYMD
                     FAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTTSIGGAYSITATHN
                     TKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGATEGADISLSKQQAL
                     ERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLNGSFVHIDDWSGWL
                     ILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNYANGKNHAAYSKWN
                     QTTIDNLKNKYSYNVDMSGAQVATIENGKLTGTGSDTTDIKNKDLIFTGGGDILLKSS
                     FDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSTVEWNIRYDNKDNLHKIGDGTL
                     DVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENALSGGEYNGIFFAKN
                     GGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSILSLNNTADYIYHGNINGNLDVLQH
                     HETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGAFSCSLPAPMRFLC
                     GSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFGTLHLENSDFSVGR
                     NANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVRGNSQGETLFTGGI
                     TAEDSTIVIKDKAKALFSNYVYLLNTKATIENGADVTTQSGMFSTSDISISGNLSMTG
                     NPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKSASIMFGHDESDLS
                     QLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGDSALKTLKSTNSMV
                     YFTDSANNKKFHTLTVDELATSNSAYAMRTNLSESDKLEVKKHLSGENNILLVDFLQK
                     PTPEKQLNIELVSAPKDTNENVFKASKQTIGFSDVTPVITTRETDDKITWSLTGYNTV
                     ANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGF
                     SDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMFD
                     SGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAE
                     LVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQ
                     FDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNV
                     DNAVNANFRYSF"
     gene            complement(4074..>4304)
                     /locus_tag="ACHJ1G_005059"
     CDS             complement(4074..>4304)
                     /locus_tag="ACHJ1G_005059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766738.1"
                     /translation="TAQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLF
                     FVDKEAGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1299 a          970 c          983 g         1052 t
ORIGIN      
        1 atcatcaaga gcaactggta agaaaaaaca caaacgcata cttgcattat gttttttagg
       61 cttattacaa tcctcatatt cttttgcgtc acagatggat atttcaaatt tctacatccg
      121 tgactatatg gattttgcac agaacaaggg catatttcag gctggcgcaa caaatattga
      181 aatagtgaag aaagatggct ccaccctgaa actaccggaa gtaccatttc ctgacttctc
      241 accggttgca aacaaagggt caaccacatc tattggtggt gcatacagta tcacagccac
      301 acacaatacg aaaaaccacc actcagttgc gacgcaaaac tgggggaaca gcacgtacaa
      361 acaaactgac tggaatactt cacatcctga ttttgcagta tcccgacttg acaagtttgt
      421 tgttgagacc cgaggtgcga ctgaaggcgc agatatttcg ttatcaaaac agcaggcact
      481 tgaacgttac ggggttaatt ataaaggaga aaagaaactt atcgcattca gagccggctc
      541 tggtgtggta tccgttaaaa aaaatggacg cataactcca tttaatgagg tttcttataa
      601 gccagaaatg ttaaatggct ctttcgttca cattgatgac tggagtggat ggctgatatt
      661 aaccaacaac cagtttgatg agtttaataa cattgcctct cagggtgaca gcggttcagc
      721 actgttcgtc tatgataacc aaaagaaaaa gtgggttgtc gctggaactg tctgggggat
      781 ttataattac gccaatggca aaaaccacgc agcatacagt aaatggaacc agacaaccat
      841 tgacaacctg aagaacaagt attcttacaa cgtggatatg tcaggagctc aggttgcaac
      901 cattgaaaat ggaaaactga caggcactgg ctcagacacc accgatataa aaaataagga
      961 cttaatattt actggcggtg gagatatcct cctgaaatcc tcttttgata atggtgctgg
     1021 cggtcttgtc tttaatgata aaaagaccta tcgagtaaac ggggatgatt tcacctttaa
     1081 aggtgccggt gttgatacaa gaaacggcag caccgttgag tggaatatcc ggtatgataa
     1141 taaagacaac cttcacaaaa ttggtgatgg cacattagat gtccgaaaaa cccagaacac
     1201 caacctgaaa acaggtgagg gtcttgtcat tcttggagct gaaaaaacat tcaataatat
     1261 ctacataacc agtggtgatg gaactgtccg actgaatgca gaaaatgcac tgtctggcgg
     1321 tgaatacaac ggtattttct ttgcgaaaaa tggcggaact cttgacctga acggatataa
     1381 tcagtctttc aataaaattg ctgcaactga ttcaggtgct gtaataacca atacgtcaac
     1441 caaaaaatcc attttatccc tgaataatac tgctgactat atctatcacg gcaacataaa
     1501 cgggaatctg gacgtacttc agcatcatga gacgaaaaaa gagaaccgtc gtcttattct
     1561 tgatgggggc gtggacacaa caaatgatat aagcctgcgt aatacacaac tgtccatgca
     1621 gggacatgcc actgaacatg ccatttatcg ggatggagct ttctcttgtt cactaccagc
     1681 tcctatgcgc tttttgtgtg gcagtgatta tgttgcagga atgcaaaata cagaagctga
     1741 tgctgtaaaa caaaacggaa atgcctataa aaccaacaat gctgtctctg atttatcgca
     1801 gccagactgg gaaaccggaa cattcagatt tggaacgcta catcttgaaa attccgattt
     1861 ttctgttggt cgtaatgcaa atgtaatcgg ggacattcag gccagtaaat caaacattac
     1921 tattggtgac actacagcat atattgattt gcatgctggt aaaaatatta ccggtgatgg
     1981 ttttggcttc cgccagaata ttgtgcgtgg aaactcacaa ggagaaacgc tgtttacagg
     2041 agggatcaca gcagaagaca gcactatcgt tattaaagat aaagcaaaag cattattttc
     2101 aaattatgta tacctgctga acacaaaagc aaccatagag aacggtgctg atgtgacaac
     2161 tcaaagtggt atgttctcca cgagcgatat cagcatctct ggtaatctgt ccatgacagg
     2221 caatcccgac aaagacaata aattcgagcc ctcaatatat ctgaatgatg cttcttatct
     2281 actgactgac gactccgcca gactcgttgc caaaaataaa gcatctgtgg tgggagatat
     2341 acactccact aaaagtgcat ccatcatgtt tggtcatgat gaaagcgacc tctcgcagtt
     2401 gtctgacaga acctcaaaag ggcttgcact tggtctttta ggtggctttg atgtctcata
     2461 tcgcggttca gtcaatgccc cgtcagcatc tgccactatg aacaatacct ggtggcaact
     2521 aaccggagat tctgcgctga aaacactgaa aagtacaaac agcatggtct atttcactga
     2581 cagcgcaaac aataagaaat tccatacgct gacggtcgat gagctggcaa ccagcaacag
     2641 cgcctatgcg atgcgtacaa acctttctga atcagacaaa ctggaggtca aaaaacacct
     2701 gtctggtgag aacaatattt tactcgttga tttccttcag aaaccaacgc ctgaaaaaca
     2761 actgaatatt gaactggtaa gcgcgccaaa agacaccaat gaaaatgtct ttaaagccag
     2821 taaacaaacc attggtttca gtgatgtaac gccggtcatt acaaccaggg aaaccgatga
     2881 caaaataaca tggtcactga caggctataa cacggtagca aacaaggaag caacccggaa
     2941 tgccgccgcc ctgttctctg ttgactataa agcgtttctg aacgaggtca acaacctgaa
     3001 caaacgtatg ggtgacctgc gtgatatcaa cggcgaagcc ggtgcatggg cacgcatcat
     3061 gagcggtacc ggctctgcca gtggtggttt cagtgacaac tacacgcacg ttcaggtcgg
     3121 ggtcgacaaa aaacacgagc tggacggact ggatttgttt accggtttca ctgtcacaca
     3181 cactgacagc agtgcctccg ccgatgtttt cagtggtaaa acgaagtctg tgggggctgg
     3241 cctgtatgct tccgccatgt ttgattccgg tgcctatatc gacctgattg gcaagtatgt
     3301 tcaccatgat aatgagtaca ctgcaacctt tgccggactc ggaacccgtg attacagcac
     3361 gcattcatgg tatgccggtg cagaagcggg ctaccgctat catgtcactg aggatgcctg
     3421 gattgagcca caggctgagc tggtttacgg ttctgtatcc ggtaaacagt ttgcatggaa
     3481 ggaccaggga atgcatctgt ccatgaagga caaggactac aatccgctga ttggccgaac
     3541 gggtgtggat gtgggtaaat ccttctctgg taaggactgg aaagtgacag cccgtgccgg
     3601 tctgggctac cagttcgacc tgctggctaa cggcgaaacc gtattgcggg atgcatctgg
     3661 tgaaaaacgc atcaaaggtg aaaaggacag ccgtatgctg atgtccgttg gcctgaatgc
     3721 agaaatcagg gataacgtcc gctttggact ggagtttgag aaatccgcct ttggtaagta
     3781 caacgttgat aatgcagtca acgctaattt ccgttactcg ttctgatact ccgtattcaa
     3841 aagggcctgc gggcccttta cgctcagtgc ctgccgccct gatatgtctg agagacattt
     3901 ccgggatgtc agtcgactgg tttacttaac tgcgatgact aaagctggca gtaatgccgg
     3961 cttttttacg gccacatacg tcttcctctc atccggaact gtgatacaaa tcctgcatac
     4021 aggattgacc gccggttcct gcaaatggca cattttcagt aagatagcga aggttacatt
     4081 cccccgtcat gtgtggcaca agctgtcacc gggaaagaaa cggaaagcac atgaaaaaca
     4141 tcaaaactca caccggcctc cttatccaca aagaacaaac gcggcgcgtt cgtcttcatg
     4201 aaacacccac tgcctggtgc cacacgcacc gggaatgcta cagcaaaacg accggtcgcc
     4261 gctgcggcag ccctgacagt ctcagcaggc tgattctgag cagt
//
LOCUS       DAZFDP010000270         4275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFDP010000270 DAZFDP010000000
VERSION     DAZFDP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            231..1163
                     /gene="rfaD"
                     /locus_tag="ACHJ1G_005060"
     CDS             231..1163
                     /gene="rfaD"
                     /locus_tag="ACHJ1G_005060"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4766739.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1173..2219
                     /gene="rfaF"
                     /locus_tag="ACHJ1G_005061"
     CDS             1173..2219
                     /gene="rfaF"
                     /locus_tag="ACHJ1G_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4766740.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2223..3215
                     /gene="rfaC"
                     /locus_tag="ACHJ1G_005062"
     CDS             2223..3215
                     /gene="rfaC"
                     /locus_tag="ACHJ1G_005062"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4766741.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3212..>4275
                     /gene="rfaL"
                     /locus_tag="ACHJ1G_005063"
     CDS             3212..>4275
                     /gene="rfaL"
                     /locus_tag="ACHJ1G_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO4766742.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVK"
BASE COUNT         1128 a          973 c         1047 g         1127 t
ORIGIN      
        1 attcaaatgc ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca
       61 tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta
      121 gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag
      181 acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg
      241 ttaccggcgg cgcgggcttt atcggcagca acatcgttaa agccctgaat gataaaggca
      301 tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg
      361 atctgaatat cgcagactat atggataagg aagacttcct gatccagatt atggctggcg
      421 aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc gtgctcttcc accaccgagt
      481 gggacggcaa gtatatgatg gataacaact atcaatactc caaggagctg ctgcactact
      541 gcctggagcg tgaaatcccg ttcctgtacg cttcttccgc agccacctac ggcggacgca
      601 cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa
      661 aattcctgtt tgatgaatat gttcgccaaa tcctgccgga agcgaactcg cagattgttg
      721 gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg
      781 tcgctttcca tctcaacacc cagcttaata acggtgaatc accgaagctg ttcgaaggta
      841 gcgagaactt taaacgcgac ttcgtctatg ttggcgatgt ggcagatgta aacctgtggt
      901 tcctggaaaa tggcgtttcc ggcatcttca acctcggcac cggtcgtgcg gaatccttcc
      961 aggctgtagc tgatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt
     1021 tcccggataa gctgaaaggc cgctaccagg cgttcactca ggcagatctg acaaatctgc
     1081 gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg
     1141 cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt
     1201 gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc
     1261 aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg
     1321 aagttaacga agctatccct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc
     1381 gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc ttacctaact
     1441 ccttcaaatc cgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg
     1501 gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc
     1561 taatggtgga acgctatata gcgctggcct atgacaaagg cattatgcgc acagcacaag
     1621 atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata
     1681 cctgtaatca attttcgctt tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg
     1741 agtttggtcc ggcaaaacgc tggccacact accactatgc ggagctggca aagcagctga
     1801 ttgatgaagg ttatcaggtg gttctgtttg gctcggcgaa agatcatgaa gcaggcaatg
     1861 agattcttgc cgctttaaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa
     1921 cacagcttga tcaagcggtt atcctgattg cagcctgtaa agccattgtc actaacgatt
     1981 ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccaa
     2041 gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg
     2101 gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca
     2161 ttactcccca gcgcgtactg gaagaactca acgcgctatt gttacaagag gaagcctgac
     2221 ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg
     2281 cactcactga tgcccagcag gcaatcccag ggattaagtt tgactgggtg gtggaagaag
     2341 ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac
     2401 gtcgctggcg taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg
     2461 aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg
     2521 cggcactggt gacacgtctg gcgcatggcg taaagcatgg attggactgg caaaccgctc
     2581 gcgaaccttt agccagcctg ttttacaatt gtaagcatca tattgcaaaa cagcagcacg
     2641 ccgtagaacg cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc
     2701 agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat
     2761 atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc
     2821 gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc
     2881 cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc
     2941 cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg
     3001 tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg
     3061 gaccaaccga tccgggatta attggtgggt atgggaagaa tcaagtagag tgtcgttcta
     3121 caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa
     3181 taataaccaa taagttgaca tcggagataa gatgacctca acattatttt tctctctcga
     3241 gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta
     3301 ttttttgggt gggattacaa ggtataaaca tcttattgtt attcttatga caataacgac
     3361 aatcgtctat ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa catttctttt
     3421 tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat cccctgatgc
     3481 aggtgctagc atgaaggaaa ttttcaaagc tattattgag aatactttac tatgcacaat
     3541 agcaataccg atcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt
     3601 ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga
     3661 ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat
     3721 ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat
     3781 ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg
     3841 ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa
     3901 gttactatta gtaggagtaa tggttgccat cattgcattg tcggttattt tcacacataa
     3961 ggagatgacc gcaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc
     4021 aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg
     4081 ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg
     4141 gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg
     4201 tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag agtgtataaa
     4261 aaatggcgtc aagaa
//
LOCUS       DAZFDP010000271         4272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFDP010000271 DAZFDP010000000
VERSION     DAZFDP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..835)
                     /gene="ygcW"
                     /locus_tag="ACHJ1G_005064"
                     /pseudo
     CDS             complement(50..835)
                     /gene="ygcW"
                     /locus_tag="ACHJ1G_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1153..2430
                     /gene="yqcE"
                     /locus_tag="ACHJ1G_005065"
     CDS             1153..2430
                     /gene="yqcE"
                     /locus_tag="ACHJ1G_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4766743.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2457..3935
                     /gene="ygcE"
                     /locus_tag="ACHJ1G_005066"
     CDS             2457..3935
                     /gene="ygcE"
                     /locus_tag="ACHJ1G_005066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4766744.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1137 a          962 c         1017 g         1156 t
ORIGIN      
        1 aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta
       61 aataaccacc atcgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga
      121 ggaatacagc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat
      181 caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat
      241 aatagccagg ggcaatacca tttacctgaa tattatattg acctagttca tcacaataag
      301 ctttggtgaa tccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac
      361 ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa
      421 tttttgcagc ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat
      481 cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta
      541 ccagaatatc aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc
      601 cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt
      661 cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg ccagctttgg
      721 ccaacgccat ggcaaatgcc tagcctaaac cgctattccc accggtaaca attgcggttt
      781 tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta
      841 gctccaattt ccgtaaggca aaaaaaagaa aggtaacttc ccccgcgaga gggaagttac
      901 ctttctcatt atctctggta actggagata atttttagca taacggcata actgctaatg
      961 tgatcgtaat cacagtgtga tattcgtatt gtcaaatagt tttaaaaagc agaagcgtga
     1021 ttactctcac acaacacctc tgctttccat gttatttctt attgcaatta ctagagacct
     1081 gagaaaagta atccacctga aagggcggct tacttttctc ttttttttgt ctattacaaa
     1141 tgggaatacg atatgcaaca caactcatat cgccgttgga taaccctcgc gataattagt
     1201 tttagcggcg gcgttagttt tgacctggct tatttacgtt atatttatca aattcccatg
     1261 gcgaaattta tgggattcag caataccgag ataggtttaa taatgagtac ctttggtatt
     1321 gcggctatta ttctttatgc ccccagcggc gttattgccg ataaattttc acaccgcaaa
     1381 atgattactt ccgcgatgat cattaccgga ttactgggtc tgttaatggc aacgtatcca
     1441 ccgctgtggg taatgctctg tattcaggtc gcctttgcga taacgacgat tttaatgctg
     1501 tggtcggtgt cgattaaagc cgcctcgttg ctgggcgatc atagtgagca agggaaaatt
     1561 atgggctgga tggaagggct gcgcggcgtc ggtgtaatgt cgctggcggt gtttaccatg
     1621 tgggtctttt ctcgctttgc accggatgac agcgccagcc tgaaaacagt cattatcatc
     1681 tacagtgtgg tttacatctt gttggggatt ctgtgctggt tttttgttag cgataacaac
     1741 aacctgcgca gtgccaataa cgaagaaaaa cagtcattcc agcttagcga catccttgcc
     1801 gttttgcgta tcagcaccac ctggtattgc agcatggtga tttttggtgt cttcacaatc
     1861 tacgccattc tgagttactc caccaactat ctgaccgaaa tgtacggcat gtcgctggtg
     1921 gcagcgagct acatggggat tgtgatcaac aaaatcttcc gcgcgctgtg cggcccactt
     1981 ggcggcatta tcaccaccta cagtaaagtg aaatccccta cccgcgtgat ccaaatcctt
     2041 tccgtactcg gcctgctggc gttaactgcc ctgctcgtca cgaactctaa cccgcaatcg
     2101 gtcgcgatgg ggattggcct gattttactg ctgggattca cctgttacgc ctcacgcggg
     2161 ctgtactggg cctgccctgg cgaagtgaga acaccgtctt acattatggg caccacggta
     2221 ggtatttgtt cggtgattgg attcctgccg gatgtcttcg tttacccgat tatcggccac
     2281 tggcaagaca ctctgcccgc agcagaagcc taccgcaata tgtggctgat gggaatggcg
     2341 gcgcttgcca tggtgattgt ctttaccttt ttgctgttcc aaaaaattcg tactgctgat
     2401 agcgcccccg caatggctag cagcaagtaa gccaggaaaa ttacaggaga aaaatcatgt
     2461 cgaaaaaata catcataggg attgatggcg gaagtcagag cacaaaagtg gtgatgtacg
     2521 atctggaagg tagtgtggtt tgcgaaggta aaggcttatt acagccgatg cacacgcctt
     2581 atgccgatac tgcagaacat cctgacgacg atttatgggc atcattatgt tttgcaggtc
     2641 acgatttgat gagccagttt gccgggaata aagaagatat tgttggcatc ggcctgggct
     2701 ccatccgttg ctgccgtgcg ttattgaaag ccgatggcac gcctgctgcg ccgttgatta
     2761 gctggcagga tgcacgcgtt acacgccctt acgaacatac gaatcctgac gtggcgtatg
     2821 tcacctcttt ttcgggttat ctgacgcatc gcttaaccgg cgagtttaaa gacaatatcg
     2881 ccaactattt tggtcagtgg ccggtggatt ataagagctg ggcatggagc gaagatgctg
     2941 cggtaatgga taagtttaat atcccccgtc atatgctgtt tgatgtgcaa atgcctggca
     3001 ccgtcctcgg acatatcaca ccacaagccg cactggcgac acatttcccg gcaggactgc
     3061 cggttgtttg taccaccagt gataaaccgg tagaagctct gggggccgga ttactggatg
     3121 atgaaactgc ggtaatttct cttggcactt acatcgcatt gatgatgaac ggcaaagcac
     3181 tgccgaaaga tccggtggcg tactggccga ttatgtcttc tattccgcaa acattgctgt
     3241 atgaaggtta cggtattcgc aaaggtatgt ggacggtgag ctggctgcgt gacatgttag
     3301 gcgagtcgtt aattcaggat gccagggcgc aggatctttc accggaagat ttactcaaca
     3361 agaaagcctc tagtgtgcca cctggctgta atgggctgat gacggtgctg gactggctga
     3421 ccaatccgtg ggaaccgtac aaacgcggga ttatgatcgg ctttgattcc agcatggatt
     3481 acgcatggat atatcgttcg atattggaaa gcgtggcgct aacgctgaag aacaattacg
     3541 acaatatgtg taatgaaatg aatcactttg cgaagcatgt gatcattact ggcggcggtt
     3601 cgaacagcga tctgtttatg caaatttttg ccgacgtgtt caaccttccg gcacgccgta
     3661 acgccattaa cggttgtgca agtctgggag cagcgattaa tacagcggta ggtctggggc
     3721 tatacccgga ttacgcaacg gctgttgata agatggttcg cgtgaaagat atctttatac
     3781 cgattgagag caatgccaaa cgctacgacg cgatgaataa aggcattttc aaagacctaa
     3841 ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt gatgcatggg gaattgggga
     3901 atgtggattc gatccagagc tggtcgaatg cgtgaatgag gtaggccggg cggaaaactg
     3961 cccggcctga acatacctaa atggttatcc ccgctgacgc ggggaacata agttcgacaa
     4021 cttaaaaaat gataacaagt ccggtttatc cccgctgatg cagggaacat aataaaaact
     4081 ttaactagtt cggtttatcc ctgtttttac aaggaatatt aactacactc gatgtcttta
     4141 gaattaaaga caatacgaat cgcactttat caattttcac ttaaagtatg aactggatat
     4201 ctttattcat aagaactatt catcaacagc gtatagaggc ggttatgtaa aaccactcat
     4261 tagcctcaaa ac
//
LOCUS       DAZFDP010000272         4234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFDP010000272 DAZFDP010000000
VERSION     DAZFDP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1660
                     /locus_tag="ACHJ1G_005067"
     CDS             <1..1660
                     /locus_tag="ACHJ1G_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4766745.1"
                     /translation="EGLPLPETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCL
                     HDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTT
                     GADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFG
                     GTPVNHAAQFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTV
                     YGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQD
                     RASHFSSWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTAL
                     LSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNG
                     AALLQKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQT
                     DAKDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQVGTPF
                     TLTQSPVSAFAADKLHVTDITRGATYPVLIDSIAVEVNSTDTAA"
     gene            1796..1975
                     /locus_tag="ACHJ1G_005068"
     CDS             1796..1975
                     /locus_tag="ACHJ1G_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4766746.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            2016..2288
                     /locus_tag="ACHJ1G_005069"
     CDS             2016..2288
                     /locus_tag="ACHJ1G_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4766747.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            2365..2580
                     /locus_tag="ACHJ1G_005070"
     CDS             2365..2580
                     /locus_tag="ACHJ1G_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4766748.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            2585..2929
                     /locus_tag="ACHJ1G_005071"
     CDS             2585..2929
                     /locus_tag="ACHJ1G_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4766749.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            2980..3513
                     /locus_tag="ACHJ1G_005072"
     CDS             2980..3513
                     /locus_tag="ACHJ1G_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766750.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            3673..3810
                     /locus_tag="ACHJ1G_005073"
     CDS             3673..3810
                     /locus_tag="ACHJ1G_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766751.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            3812..4174
                     /locus_tag="ACHJ1G_005074"
                     /pseudo
     CDS             3812..4174
                     /locus_tag="ACHJ1G_005074"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            4146..4217
                     /locus_tag="ACHJ1G_005075"
                     /pseudo
     CDS             4146..4217
                     /locus_tag="ACHJ1G_005075"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
BASE COUNT         1078 a          977 c         1260 g          919 t
ORIGIN      
        1 tgaaggcctt ccgctgccgg agacatatga ccgtccggac ccgcgtatta agcagctggc
       61 gcgccgcagt acggtgacac cgggcggtgc agcatgcaaa tataacgaca tcattccggc
      121 ggaccattgt ctgcatgatg tgcaggacat gagccgcctt aaccatccga aagcggacct
      181 gtcaaagggg cagtacggaa ccgtggggca agggctgcat atcgccaaaa aattgctgcc
      241 gtttataccg gcgaatgcgg gcattctgct ggttccgtgc tgtcgtggtg gttcagcgtt
      301 caccaccgga gccgatggca catacagtga cgcgagtggt gcctcggaga attcaacccg
      361 ctggggtgtg gacaagccgc tgtataagga ccttatcggt cgaacaaaag cagcactgaa
      421 gaagaacccg aaaaatgtgc tgtttgccgt ggtgtggatg cagggggaat ttgattttgg
      481 cggtacgccg gtaaatcacg cagcacagtt tggtgcgctg gttgataaat tccgtgcaga
      541 cctggcggat atggcaggtc agtgcgtcgg tggctctgct ggcggtgttc cctggatatg
      601 tggagatacg acgtatttct ggaagcagaa gaacgaatcc tcgtaccaga cggtgtacgg
      661 cagctacaaa aacaaaacgg aaaagaatat ccatttcgta ccgttcatga cggatgagaa
      721 cggggtgaat gtgccgacga acaaaccgga agaagacccg gacattccgg gtatcggata
      781 ttacggttcg aaatggcgtg acagctcagc cacctggacg tcacaggaca gggcgagcca
      841 tttcagttca tgggctcgcc gcgggattat ttccgaccgt ctggcaacgg cgattttgcg
      901 ccatgcggga agagtggcgc taaacgcggg ggcatcatcg acagtatcag aggtgcgccc
      961 gtcatcgcct tccggtgcag aagccacagg cgtcacagca ctgctctctt accttgccag
     1021 cgagtcagag ggaagcctga aagtacaggg atggtcagcc agtggcggca gggcagaagt
     1081 ggtcagcgat gcggagggaa ccggaggtaa ggcagtgaag ctgaccaagg aagccggtaa
     1141 aagcagctgg gtgctggagt acgccgcggg caacggtgcg gctctgttac agaaaggggg
     1201 gcagattcgc tgccgcttta aggtttcggg agcgctggct gcgaaccagt atgttatggc
     1261 gttttactgg ccggtatctt cactgccaca gggcgttgcc ctgaccggag acggggggaa
     1321 taacctgctg gcagcgttct acatccagac agatgcaaaa gacctgaatg tgatgtacca
     1381 caatgcgaaa gtggcgacaa acaacctgaa actgggaacc tttggcgcat ttgataacga
     1441 atggcatacg ctggctttcc gctttgccgg gaataacagc ctgcaggtga cgccggttat
     1501 tgatggtcag gttggcacac cgttcacgct gacgcagtca ccggtcagtg cctttgcggc
     1561 ggataaactg catgtgacag acattaccag aggtgcgact tacccggtac tgatagacag
     1621 cattgcggtg gaagtgaaca gcacagacac tgcggcatga taaaaaaacc gccagcgaca
     1681 ggaatggacg ctggcggtgg tgatacctat ggagaaaaaa taaaggaacg atactttcgt
     1741 actctggttt tttaatgaaa acagttctta ttgtcaacaa taacggaaag aaattatgac
     1801 atttctgaac cagttaatgc tgtacttctg tacggtggtc tgtgtgctgt atctcctttc
     1861 gggtggatac cgggccatgc gtgacgtctg gcgcagacag attgacaaaa gggccgctga
     1921 gaaaatcagc gccagtcagt cagccggaag caaacccgaa gagccgctca tttagcggca
     1981 actttcttaa tcacatcttt cgacgagaaa atcccatgtc agaaattaca tccctggtca
     2041 ctgctgaagc agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca ctgaaacaaa
     2101 aacttcgcca taacctggaa gcgcgtcttg atgcagaggt tgatgccatt ctggatgagc
     2161 tgcttggtgt acaggcagag ccaccgactg aagcgggaga taccaccgca gagagcggtg
     2221 aagttcagcc tgaatcaccg gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga
     2281 tgctgtaacg gggagtcagg gccatcagta gttaactggt ggctttttta ttgttgtcag
     2341 cttccggata acgggagacg gggtatgtac cagatggaaa aaatcacaac gggtgtgtca
     2401 tacaccacgt cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct
     2461 ccgtcccagt gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat
     2521 cttactaacc tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggaaagtaa
     2581 agcgatgaag aaaaaatacg aactggttgt taaagggata aataattacc cggataagat
     2641 tactgttact gtggcactgg aaattggtgg gtatccgtca ctgttgttgc cagatgtggc
     2701 gattagtctt gaccgtactg aaggtgccac gctggagttt tacgaagctg aggcgaaaaa
     2761 gcaggcgaag cagtttttca tggatgttgc tgccgggtta tgtgaagggg atggtccgtt
     2821 gccggaaaag cggcccatca ttttagaggc gcaggatgtg ttgataacct acagaggaaa
     2881 actaccggga ataattactg gttctctgaa gactccaccg ctggcctgaa gacttaatat
     2941 atccagggat ttgaaatcga taaaccctga taaatatcca tgaacgcaaa aatcagatac
     3001 ggcctgtcgg ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc tgaaatcctc
     3061 gaccagtttc ttgacgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggg
     3121 atctggacca tctgccgcgg tgccatcctg gtggatggta aacctgtcgt cccgggcatg
     3181 aagttgtcga aggaaaaatg cgaccaggtt aacgccattg aacgtgataa ggcgctggca
     3241 tgggtggaga aaaacatcag agtgccactg accgaacccc agaaagcggg gattgcgtca
     3301 ttctgtccgt acaacattgg ccccggtaag tgtttcccgt cgacgtttta cagacggatt
     3361 aatgccggtg accgcagggg agcatgcgag gcgattcgct ggtggattaa ggacggtggc
     3421 agagactgcc gtattcgctc aaacaactgc tacggtcagg tctcacggcg tgaccaggag
     3481 agcgcgctgg cgtgctgggg aattgacaga taagcagaat attttgctga aaaatgcgat
     3541 ttgctcacgc ggacggataa cacgaaatcc tgcgaactga caaaaactaa gtgaataaaa
     3601 gtaaaaaccc cgtttgttgg ctgcaagcgg ggttttgtgt ttcctgactc cggaaaagtc
     3661 aaaggagaaa gtgtgtttga ttttagcaaa ctgattcggg agattcgaat gatggctgaa
     3721 aaattatcca cctggaagtt catccttatc tggctggtgt ttgtgattat ggcttccggt
     3781 tatttcattg gtcagatacg ctggtggtga aatgaaccgc gtactgtgcg tggtcatcat
     3841 tgccctgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc gtgataacgc
     3901 cataacctac aaagagcagc gcgataaaaa agtcagtgag ctggagctgg caaatgcaac
     3961 cattactgat atgcagcagc gccagcgtga tgttgctgca cttgatgcca gatactcgag
     4021 ggaattagcc gatgcgagag ctgaaaatga aactctgcgc gctgatgttg ccgctggtcg
     4081 taagcgcctg cggatcaacg ccacctgccc cggtaccgtg cgtgaagcca ccggcacctc
     4141 cggcgtggat aatgcaaccg gcccccgact ggcagacacc gctgaacggg attatttcat
     4201 cctcagagaa cgattgatga caatgcagaa gcag
//
LOCUS       DAZFDP010000273         4202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFDP010000273 DAZFDP010000000
VERSION     DAZFDP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..985
                     /gene="ybeF"
                     /locus_tag="ACHJ1G_005076"
     CDS             32..985
                     /gene="ybeF"
                     /locus_tag="ACHJ1G_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4766752.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1194..2159
                     /gene="lipA"
                     /locus_tag="ACHJ1G_005077"
     CDS             1194..2159
                     /gene="lipA"
                     /locus_tag="ACHJ1G_005077"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4766753.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2261..2464)
                     /gene="tatE"
                     /locus_tag="ACHJ1G_005078"
     CDS             complement(2261..2464)
                     /gene="tatE"
                     /locus_tag="ACHJ1G_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4766754.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2593..3381)
                     /locus_tag="ACHJ1G_005079"
     CDS             complement(2593..3381)
                     /locus_tag="ACHJ1G_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4766755.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3474..3857
                     /gene="crcB"
                     /locus_tag="ACHJ1G_005080"
     CDS             3474..3857
                     /gene="crcB"
                     /locus_tag="ACHJ1G_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4766756.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3912..4121)
                     /gene="cspE"
                     /locus_tag="ACHJ1G_005081"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3912..4121)
                     /gene="cspE"
                     /locus_tag="ACHJ1G_005081"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4766757.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1100 a         1072 c          962 g         1068 t
ORIGIN      
        1 atttttccat gaataaaatt ggagtaagtg cgtggatagt aataatcaaa ttgaaccctg
       61 tttaagcaga aagtcgtcag aaggtaaacc acaaatattt acaaccttac gaaatattga
      121 tcttaacctt ctgactattt ttgaagctgt atatgtacat aaagggatcg ttaatgcagc
      181 gaaagtgctt aatctgaccc cctcggcaat cagtcagtct attcagaaac tgcgcgttat
      241 attccctgac ccattgttta ttcgcaaagg ccagggtgtc actcctaccg catttgcgat
      301 gcatctacat gagtatatca gtcagggcct tgagtccatt cttggcgcgc tggatatcga
      361 aggaagctat gataagcaac gaacgataac tattgcgacc actccctcgg tcggagccct
      421 ggtccttcct gtcatctatc gggcgattaa aactcactat ccgcagcttt tactgcgcaa
      481 cccgcccatc agcgacgcgg aaaaccaact cagtcagttt caaaccgatc tcatcatcga
      541 taacatgttt tgcaccaatc gtacggtgca acatcatgtt ctgttcaccg acaatatggt
      601 gttaatttgc cgcgagggaa atccactact ctctttagaa gatgacagag agactatcga
      661 caacgctgcg catgtacttc tgttaccgga agagcaaaat ttcagcggtc tgcggcagag
      721 agttcaagaa atgtttccgg accggcaaat caatttcacc agctacaaca ttttgacaat
      781 cgctgcactg gttgccaaca gtgacatgtt agcgattatt cccagccgtt tttataacct
      841 gtttagccgc tgctggccgc tggaaaaact gccttttccg tccttaaatg aggagcaaat
      901 agatttctct atccactaca acaaattcag cctgcgtgat cccatcctgc acggagtaat
      961 agatgttatc cgtaatgcat tttgaaaaag tattgttaca gccagatcac agcgcagaac
     1021 gataaaatgg cacaatccaa caacaatttt acattttcgc gaccgctttg gctgctttca
     1081 ggtccgtttc aatgatatac tgccagtcgt taattcaaaa atagttgata attacaacaa
     1141 tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg gaacacgcac gctatgagta
     1201 aacccattgt gatggaacgc ggtgttaaat accgcgatgc cgataagatg gcccttatcc
     1261 cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg caagccggaa tggatgaaaa
     1321 tcaagcttcc agcggactct acacgtatcc agggcatcaa agccgcaatg cgcaaaaatg
     1381 gcctgcattc tgtctgcgag gaagcctcct gccctaacct ggcggaatgc ttcaaccacg
     1441 gcacagcaac gtttatgatc ctcggcgcta tttgtacccg ccgttgtccg ttctgtgatg
     1501 ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc agtgaaactg gcgcagacca
     1561 ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt tgaccgtgat gacctgcgcg
     1621 atggcggtgc ccagcacttt gcggattgca ttactgccat tcgggaaaaa agcccgcaaa
     1681 tcaaaattga aactctggtg ccggatttcc gtggtcgtat ggatcgtgct ctggatattc
     1741 tgactgcaac gccaccagat gtgttcaacc ataacctgga aaacgtaccg cgtatttacc
     1801 gtcaggtacg gcctggtgca gattacaact ggtcgctgaa gctgctggaa cgctttaaag
     1861 aagcgcatcc ggaaatcccg accaagtctg gtctgatggt gggactgggt gaaaccaatg
     1921 aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg tgtgacgatg ttaacgctgg
     1981 ggcaatattt gcagccaagc cgccatcacc tgccggttca acgttacgtt agcccggatg
     2041 agttcgacga aatgaaagcc gaagcgctgg cgatgggctt tacccatgct gcatgcggtc
     2101 cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa agggatggaa gttaagtaat
     2161 cctgtaataa ttacttacat ctgtctgata aaaaaacccg ctttttgaag cgggtttttt
     2221 gtatcgaaca agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct
     2281 tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg
     2341 ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag
     2401 tcccaaacag cagaacgacc agcgccgcaa ctactagcag tttggtaata ctaatctcac
     2461 ccatagatac cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata
     2521 ttaacggtca ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg
     2581 agtaaaaaaa catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag
     2641 ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat
     2701 ttctgacgct gccgcaatgg tgacgccaaa gggatctata atccggcttt gaccgatatt
     2761 tttgttcccg cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc
     2821 aagcaacgtt gaccaatgat gctctttgag cggcccgcga acccaggcgg caggaagtac
     2881 caggatttca gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata
     2941 acaggtcatc agaccgacct tcatcccctc cacctcgagt aacggagcga tttcattacc
     3001 agcatcaaca cggcgtgatt cctgaatggc aaatgcatca tagagatgca atttggcata
     3061 acgggcgacg atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt
     3121 tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa
     3181 acgtccgagg aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg
     3241 atcatcgcgc gccagcaatg cttccggcag gacgaacagc gatacgtcgt tttccgccgc
     3301 ctgcgccatc aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa
     3361 ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg
     3421 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
     3481 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
     3541 gtatgcgatt taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga
     3601 ttggggcatt catcatagga atgggattcg cctggttcag caggatgacg aacattgatc
     3661 cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
     3721 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggcg ttactgaacg
     3781 ttttcgtcaa ccttctgggg tcttttgcca tgacagcact ggctttctgg ctgttttcgg
     3841 cctcaaccgc acactaaaga ccaaaaaaaa acccgctgat taagcgggtt ttgaattctt
     3901 gctgacgtat cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg
     3961 atttcgaact ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca
     4021 gagaagtgta cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg
     4081 gactcattaa accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga
     4141 aaaatagaca caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag
     4201 tg
//
LOCUS       DAZFDP010000274         4200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFDP010000274 DAZFDP010000000
VERSION     DAZFDP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            292..1041
                     /locus_tag="ACHJ1G_005082"
     CDS             292..1041
                     /locus_tag="ACHJ1G_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO4766758.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            968..3090
                     /locus_tag="ACHJ1G_005083"
                     /pseudo
     CDS             968..3090
                     /locus_tag="ACHJ1G_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3260..>4200)
                     /locus_tag="ACHJ1G_005084"
     CDS             complement(3260..>4200)
                     /locus_tag="ACHJ1G_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4766759.1"
                     /translation="GDAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLT
                     LVTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQV
                     VGLTMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEV
                     REEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARA
                     LVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTR
                     WPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1246 a          959 c          916 g         1079 t
ORIGIN      
        1 tactagcctg aactatattt attctgctgc aatcaatgca tacataacac aaatatcact
       61 caggtacact actcaaacca cgctgggatt tttcctcaag ttataatcat caccccgaaa
      121 atcattcggc atttactcat taaatagtca ccccataggc ctgtacatgt ttactcagaa
      181 atatacatcc ttttctctgt cataaaccct ctgattaatc ataaataaat acttgtgaca
      241 ccaatctttt tccttaacgg aacgaattgt tgtgtagaag gagataatat tatgtcaaaa
      301 aaatatcagc tattgcttat aactcattat atgtcaacat gggtcactat aacggaagca
      361 gttgagatca ctaccaaagc cataaaacaa aaaattactc ctagcgatat ttatcgtcat
      421 gccttgagtg gcaacatcct actattggtc tatttccagt ctcctgtgat acttaagaag
      481 atacaaactt ttaatggaaa aataaaattc aggcagtttg tgggagacct acttgataaa
      541 ctatgcatgc ttgacaggga cggttttatt tatggtcaaa atttaagact ctgtacagaa
      601 gccagatata tttgtcctgt gcaacaaatt atagataccc ccctgttacg caaactcaac
      661 cagtttcgta cgtttgttcg taatgtccgc ccgggtgatg aactggatgt ccaggcacaa
      721 gttagtgaaa aaaatttaac cccgccgccg ggtaacagca gtggcaacct tgagcaacag
      781 atagccagta cttcacagct aattggatct ctgctcgccg aagatatgaa cagcgagcaa
      841 gcggcaaaca tagcacgtgg atgggcctct tctcagactt caggggtaat gacagactgg
      901 ttaagccgct tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt cagcctgaag
      961 aactcccgtg acggcaaccc tcgaaaatgg cgattcgatg caacaaacag tgaactatgt
     1021 gccgaacgtc acgaatgctg aaatcacgct ggcagccttg aaggatccgg tgattgccga
     1081 caataacgat ctcacgacac taacagcacc gtcgctgata cagagggcaa tgcgatagcc
     1141 aacactgagg taacatttac tctaccggaa gatgtgaagg cgaacttcac gctgagcgat
     1201 ggcgacaaag cggttactga tgccgatggc aaagcgaaag tcacgctgaa aggtacaaaa
     1261 gcaggcgctc ataccgtgac agcatcgatg gttggcggta agagtgagca gttagtggtg
     1321 aactttactg cggatacgct cactgcgcag gttaatctta acgttaccga ggacaatttc
     1381 atcgccaata acattggaat gaccaggcta caagcaatag tgactgatgg aaacggcaac
     1441 ccagtagaag gtataaaagt taatttccgt ggaacctccg tcacgctaag cagcaccagc
     1501 gttgaaacgg atgatcaggt ttttgctgaa attcttgtga caagcaccga ggtcggacta
     1561 aaaacagttt cagcctctct ggcagataaa cctactgaag tcatctcgcg attactgaat
     1621 gccaaggtag atgttaattc tgcgacgatt accagtcagg agatacctga aggtcaggtc
     1681 atggtcgcac aagacatagc agttaaagct cacgttaacg accagtttgg caacccggtg
     1741 acgcatcaag ccgcgacatt cagtgcagcg ccatcctcgc aaatgatcat cagccagaac
     1801 acggtctcta ctaatacgca gggagtagcc gaggtcacca tgacgtctga aagaaacggc
     1861 tcgtatactg taaaagcatc cctagcgaat ggagcttcac ttgagaaaca actggaggct
     1921 attgatgaaa aactgacact cacgtccagt ccgcttatcg gtgtcaatgc tcctaaaggc
     1981 gctactctga cggcaacgct aacctctgca aatggcactc cagtggaggg tcaggtcatc
     2041 aactttagcg taacgctaga aggtgcgacg ttaagtggcg gaaaagtgag aaccaactct
     2101 tcaggtcagg ctccagtcgt tctgaccagc aataaagtcg gtacatatac ggtgactgca
     2161 tcgttccata acggcgtcac aatacagaca cagacaaccg tgaaggttac tggcaaccca
     2221 agcaccgcac atgttgctag ctttatcgct gatccgtcga ctatcgccgc caccaacagt
     2281 gatttaagta ccttaaaggc aacggttgag gatggcagtg gtaacctgat cgaaggtctc
     2341 actgtgtact tcgccttaaa aagcggctcc accacattaa cgtcattaac agcggtgacc
     2401 gatcaaaacg gaatcgcgac aacaaacgtg agatgagcga taacagggag cgtcactgta
     2461 agcgcagtca taagcgcagg tggaatgcaa acagtagata tatcgctagt ggcaggccca
     2521 gcagacgcct cgcagtccat ccttaagaac aaccagtcat cactgaaagg ggactttacc
     2581 gatagtgctg agctacatct tgttctgcac gatatatcag gcaatccgat caaagtttct
     2641 gaagggatgg aatttgtgca atcaggtaca aacgtgccct atatgaaaat tagcgcaatt
     2701 gattacagtc aaaatatcaa tggtgattac aaagctactg ttacaggaga cggagagggt
     2761 atcgcaacgc tgatccccgt attgaatggt gttcatcagg cgggcctgag taccacgata
     2821 gaattcatta gcgcggaaac cagaccaatg acgggtacag tgtcagtgaa tagtgcaaac
     2881 ctaccgacag cttcattccc ttcgcaggga tttaccgggg cgtattatca gttgaataat
     2941 gacaactttg caccaggaaa aacggcggct gattattcgt tttcaagctc ggcctcctgg
     3001 gtaggcgttg atgctactgg taaagtgaca ttcaaaaatg acggtgatag caatacagta
     3061 ataatcacag cgcccccaag atccggtggt gctatttacc aaacagttcc tcctgaatcg
     3121 cgttcggtgt gactatgatc attttcggtg agtattgctt tgatccaaag gctggttttc
     3181 tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca gatcggcatt
     3241 actatgcata agtactgcct caaattctcg ccaaaacact ttttaatgcc tttgcgaatt
     3301 catccagact tatattgtca attttttgcc caacgacgac tgactccgaa acatcggcca
     3361 ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacaggggta ttataggcac
     3421 ttgccatatg taatattgag gtgtcaggag ttattgcaat ataagcatct tttatgattg
     3481 ctgctgaacg tttaatcgag ggtgataagg ataaacggta gacattatta taacaatcca
     3541 ctaatgccct ggctttatct tcccctttgg gtccataaac gatcactatt ggaatatctg
     3601 tttcactttg aatttttgct attagatttt ctgcaatcga taatgaaaat gtacgttcct
     3661 gcgagctacc gtctaaattg agcgcaatgt aagatcctaa cgatcgcatt tcctcgcgta
     3721 cctcatcgag tacatcatcg cttaatggca attcatatat tgacctgact gccggaaaac
     3781 ccgcctcacg catcataaat gcccatgtca tgggtacagg agtccggaga ttctgatcca
     3841 tccgggacgc gttcttacac aaaggggaat agcacttcat ggttaaaccg acgacctgga
     3901 aattcgtttt tgctttcaac tgactgataa atatcatcgt tttcaggttc tttttacgca
     3961 tcgcctctat acaaagatcc ggcgtaccgt attgcagtgt tatattcttc gctaaatctt
     4021 ttatttcttt taatgttgcg tgatcctgca tagtcattgt gactaatgtt aatttagtct
     4081 gttcaagttt gagcgcatta aagacttcta aattaattgt cgacgtaaca attaatagat
     4141 gcttaatttt atgcaattca agcgcccgaa taacaggaaa gatggccata gcatcgccaa
//
LOCUS       DAZFDP010000275         4147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFDP010000275 DAZFDP010000000
VERSION     DAZFDP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..944
                     /locus_tag="ACHJ1G_005085"
     CDS             <1..944
                     /locus_tag="ACHJ1G_005085"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO4766760.1"
                     /translation="KKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRTVICLDI
                     TNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGCCSSYIANLLFFNS
                     EYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSINESKT
                     TIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYHDLW
                     NRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDE
                     QIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            949..1152
                     /locus_tag="ACHJ1G_005086"
     CDS             949..1152
                     /locus_tag="ACHJ1G_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HFO4766761.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(1989..2183)
                     /locus_tag="ACHJ1G_005087"
     CDS             complement(1989..2183)
                     /locus_tag="ACHJ1G_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4766762.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            2467..2790
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005088"
     CDS             2467..2790
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO4766763.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            3040..3378
                     /locus_tag="ACHJ1G_005089"
     CDS             3040..3378
                     /locus_tag="ACHJ1G_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766764.1"
                     /translation="MNISIKNNYLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKK
                     IATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGI
                     VVMAFVAELI"
     gene            complement(3927..>4147)
                     /locus_tag="ACHJ1G_005090"
     CDS             complement(3927..>4147)
                     /locus_tag="ACHJ1G_005090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766765.1"
                     /translation="IPFKDLLGSKICLSFLFFPVSTLNCLYDIDTDLTIQSNLFLSMP
                     NIADKESKDATGVPLNLGLFMMFELKVD"
BASE COUNT         1144 a          778 c          987 g         1238 t
ORIGIN      
        1 aaaaaaaaat aaataaagaa atttttgaga aaattgatta ccctcattat ttgcatgggg
       61 cgttaagtgg tcgcgattat atatctaatg ccgctgttca tactaggaaa agaacagtta
      121 tatgtcttga tatcaccaat ttttatccca gcattagtaa aaaagatgtt tgttctattt
      181 ttaaaaattt aatgcgtttt tctcctgagg tttctttgtg cttaacggaa cttgtgacgt
      241 tgaataataa ggttcctcag ggtgggtgct gttctagtta tattgcaaat cttttatttt
      301 ttaatagtga gtataatctc tataatagat tgaagtctat ggggctttca tattcaaggc
      361 ttttagatga tattactata tcctctgata aggatctttc atcagaggaa aaaacaaaag
      421 taataaaact cgttcacgga atggtgaatc agtacagact ttcaataaat gaaagtaaga
      481 caacgataga gcactcaaaa gattcgtctt ctaaacttag tgtcactggg ttgtgggtaa
      541 agcatggcgt tcctaagtta acaaaagaaa atagacgtta tatcaggtac ttagtttata
      601 tatgtaaaaa acaaggtgct tatgaacgtc atacgaaaga gtaccatgat ttatggaata
      661 gatgctctgg caaggttgct caaatgtcga ggcttgggca tgtccaggcg gttgaattga
      721 gggcgattct ttctgagata atgccagttt atgatgatta caagatatct aaattaaagt
      781 tgatggctaa gcattatcta aataaattta cgccgccttt aactgatgag cagataagga
      841 aaatagatag gatgctctat gattttgata tagtagggcg gactaataag aatcttgcaa
      901 aactatatag acgtaagctt gtggcattac ttccagatag gtgattatat ggaaaatgga
      961 tttgttaatt tttatgacca tgttaaaggg tatggcttta taaggcgtga gcgtggacgt
     1021 gatgttttct ttcggtatga tgattttttg tttttagggc atgatgtcga tatatgtaaa
     1081 ggtattttag ttaggtttaa attagaaaaa acagataaag ggtttaaagc tgtagcgatt
     1141 caaaaggtat aacatcgtgg atgtgtcagc ttgtttttat gatatgagaa cttgagatcc
     1201 tttctatctc gtcgtaatct cttgctgtgt aaacgaaaaa accgccttgc agggcggttt
     1261 ttcgaaggtt ctcagagcta ccaactcttt gagccgaggt aactggcttg gaggagcgca
     1321 gtcaccaaaa cttgttcttt cagtttagcc ttaacaggcg catgacttca aggctaactc
     1381 ctctaattcg gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt ctcggtttgg
     1441 attcaagtcg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1501 tacagtccag cttggagcga acgacctaca ccgggccgag ataccaacag cgtgagctat
     1561 gagaaagcgc cacgtttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc tcctgctcgc ccttcttctg ggcctttgtc ttttgctcac
     1801 atgttctttc cggctttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1861 gttgacaccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     1921 gaagagcgcc ttatgtgaca ttttctcctt acgctcctct atgccgttct gcatcctgtc
     1981 ctgatgcgtt atatcccggt gcgattttcc gcttcaaagc gtgtctgtat gctgttctga
     2041 agttcttccg cgagttcgtg cagcttctca cacatggctg cctgttcgtc agcatcaagt
     2101 gcgtccagtt tttcgagcag cgtcaggctc tggcttttta tgaatcctgc catgttgagt
     2161 acggtttgct gctgcttgtt catctttctg ttttctccgt tctttctgtc atctgctttg
     2221 tgtgattata tcgcgtacca cttttcgact gttttaccgt cggtattctg ctgtctgact
     2281 ctttgacggg catttctgtc agacaacact gtcactgcca aaaaactgcc gtgcctttgt
     2341 cggtaattcg agcttgctga caggacagga tgtgtaattg ttataccgcg catgcatgca
     2401 cgctattaca attaccctgg tcagggcttt gccccgacac ccatgtcaga tacggagcca
     2461 tgttttatga caaaacgaag tggaagtaat acgcgcaggc gggctatcag tcgccctgtt
     2521 cgtctgacgg cagaagaaga ccaggaaatc agaaaaaggg ctgctgaatg cggcaagacc
     2581 gtttccggtt ttttacgggc ggcagctctc ggtaagaaag ttaactcact gactgatgat
     2641 cgggtactga aagaagttat gagactgggg gcgttacaga aaaaactctt tatcgacggc
     2701 aagcgtgtcg gggacaggga atatgcggag gtgctgatcg ctattacgga gtatcaccgt
     2761 gccctgttat ccaggcttat ggcagattag cttcccggag agaaaactgt cgaaaacaga
     2821 cggtatgaac accgtaagct cccaaagtga tcaccattcg ctttcatgca tagctatgca
     2881 gcgagctgaa aacgatcctg acgcatcctt cctgtttttc cggggtaaaa catctctttt
     2941 tgcggtgtct gcgtcagaat cgcgttcagc gcgtttcagt ggtgcgtaca attaagggat
     3001 tatggtaaat acaattggtt aaattactat tggtgataaa tgaatatttc aataaaaaac
     3061 aattacttaa aaactattgc tcggtttgac ggtaaaacct taagagatgc cttttcgaga
     3121 aaaatagcga cttttgatgg caagatttta agggatgcta gcttaaaaaa aatagcgact
     3181 tttgatggca aggttttaag ggatgcctct ttaaaaaaag tggcgacttt tgatggcaag
     3241 gttttaaggg atgcctcttt aaaaaaagta gcaacctttg acggtcagaa tttaagagat
     3301 gctagttcaa aaattatcct gagtgccgat gctgaggttg ggattgttgt tatggccttt
     3361 gtagctgagt tgatttgaat ctcatgctgc atacctttta gcctatcgct ggcaggataa
     3421 ggcgctttct tgttggtgac agaaagataa aaaactttat aataatcagc gaagcgggcg
     3481 aaccacgcct ggctctcgct ctttaacaaa tggttacgtg tatgttgcgc aaggaaaccg
     3541 atgccttgtt tttaaggcga tctcgcatga gatcatgacc ggcctggccg ctaattaatg
     3601 gtgagaacgc ccctgagcgt gtatcagcac taaaagaata gcgacgattg tctggcaatg
     3661 tgagttaaaa gtcccaaaga gccaatagag tttttccttg ctttcctact ggtctggcaa
     3721 aatttcataa ccaataggag ttgcatcatg tttattatca acatcaacat taacgtaacc
     3781 aagagctctt catcttgttc tggagacggt cagatgaaga aaaaaagaga ggttggctct
     3841 ctggaggcac tgaaaaaaac acagtttttg ttttctatgt gcttgattat gtttaaagta
     3901 atagattacg tatcgaaact ttttaactaa tcgactttta actcaaacat catgaataaa
     3961 cccaggttta atggaactcc tgttgcatct ttggattctt tatctgcgat gttaggcata
     4021 gaaagaaaaa gattggactg gatagtcaag tctgtatcga tgtcgtacaa gcagtttaag
     4081 gttgaaacag ggaagaataa aaaagaaagg caaatttttg aacccaagag atctttgaaa
     4141 ggaattc
//
LOCUS       DAZFDP010000276         4083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFDP010000276 DAZFDP010000000
VERSION     DAZFDP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..1704
                     /gene="dpiB"
                     /locus_tag="ACHJ1G_005091"
     CDS             46..1704
                     /gene="dpiB"
                     /locus_tag="ACHJ1G_005091"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4766766.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1673..2353
                     /gene="dpiA"
                     /locus_tag="ACHJ1G_005092"
                     /gene_synonym="citB"
     CDS             1673..2353
                     /gene="dpiA"
                     /locus_tag="ACHJ1G_005092"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4766767.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2394..3779)
                     /gene="dcuC"
                     /locus_tag="ACHJ1G_005093"
     CDS             complement(2394..3779)
                     /gene="dcuC"
                     /locus_tag="ACHJ1G_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4766768.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1124 a          954 c         1052 g          953 t
ORIGIN      
        1 aattacatcc gtcaaagagg ctcgggacaa cccgcaagga aaacaatgtt gcagcttaac
       61 gagaataaac agtttgcatt tttccaaaga ctggcatttc cgctgcgtat ctttttgctg
      121 attctggtgt tctcaatatt tgtcattgca gccctggcgc aatattttac ggccagtttt
      181 gaggactatt taacgcttca tgtacgcgac atggcaatga atcaggcgaa aattattgcc
      241 tccaatgaca gtgtcatctc tgcggtgaaa acgcgtgact acaaacggct ggcgaccatc
      301 gctaacaaat tacaaagaga taccgatttt gattatgtgg tgattgggga ccggcactcg
      361 atccgccttt accatcctaa tccggagaaa attggttatc ctatgcagtt caccaaacag
      421 ggcgcgctgg agaaagggga gagctacttc attaccggga aagggtcaat ggggatggcg
      481 atgcgcgcca aaacgccaat ctttgatgac gatggaaaag tcatcggcgt ggtgtcgatt
      541 ggctacctgg tgagtaaaat cgatagctgg cgggctgagt ttttattacc gatggcaggc
      601 gtgtttgtcg tgctgttagg gattctgatg ttgctgtcgt ggttcctggc agcgcatatc
      661 cgtcggcaaa tgatgggcat ggagccaaag caaatcgcac gcgtggtccg tcagcaagag
      721 gcgctgttta gttcggttta tgaagggctg attgcggtgg atccgcatgg ttacattacc
      781 gccatcaatc gtaacgcaag aaagatgctg gggctgagct ctcccggacg gcaatggttg
      841 ggtaaaccca ttgctgaagt ggtcaggccc gccgatttct ttaccgaaca gattgatgaa
      901 aaacgtcagg atgtggtggc taactttaac ggtctgagcg ttattgccaa tcgggaagct
      961 attcgttcag gtgatgattt gctgggggcc attatcagct ttcgtagtaa agacgaaatt
     1021 tccaccctca atgcgcaact gacgcaaata aaacaatacg tcgagagcct tcgtacattg
     1081 cgacacgagc atctcaattg gatgtcgacg ctcaatggtc tgttgcagat gaaagagtat
     1141 gatcgcgtgc tggcgatggt gcagggggag tctcaggccc agcaacagct tattgacagc
     1201 ctgcgtgagg cgtttgccga tcgccaggtg gcggggctgc tttttggtaa agtgcagcgc
     1261 gcccgggaac tggggctaaa aatgatcatt gtccccggta gccagctttc gcaactgccg
     1321 ccaggactgg atagcaccga gtttgcagcc attgtgggca atttacttga taacgccttc
     1381 gaagccagcc tgcgtagcga tgaaggaaac aagatcgttg aattattcct cagcgatgaa
     1441 ggcgatgatg tggtgattga agtcgccgat cagggctgcg gcgttccaga gtctctacga
     1501 gacaaaatat ttgagcaggg ggtcagtacg cgtgctgacg agcccggtga acatggcatt
     1561 gggttgtact tgattgccag ctacgtaacg cgctgcggtg gtgttatcac tctcgaagat
     1621 aatgatccct gcggtacctt gttttcaatc tatattccga aagtgaaacc taatgacagc
     1681 tccattaacc ctattgatcg ttgaggacga aacgccgctg gcagagatgc atgcggaata
     1741 tattcgtcac attcccggat tcagtcagat attactggcg ggaaacctgg cgcaggcccg
     1801 aatgatgatc gagcgtttta agccggggct aatcttgctc gataactatc ttcctgacgg
     1861 cagagggatt aatttattgc atgaactggt gcaggcgcat tatcccggcg acgtggtgtt
     1921 taccactgca gccagcgata tggaaacggt gtctgaagca gtacgttgtg gtgtatttga
     1981 ttatctcatt aagcccattg cctatgaacg gctggggcaa acgctaaccc gtttccgcca
     2041 gcgtaaacat atgctggaaa gtattgatag cgccagccag aagcaaattg atgagatgtt
     2101 taatgcctat gctcgcggtg aacctaaaga cgaactgccg accggcattg atcccttgac
     2161 gctaaacgcg gtgcgaaaac tgtttaaaga gcctggtgtg caacatacgg cagaaacggt
     2221 ggcgcaggca ctgaccatca gtcgcaccac tgccaggcgt tatcttgaat attgcgccag
     2281 ccgccatctg attattgctg aaattgttca cggcaaagtt ggcagaccgc aacgcatata
     2341 ccacagtggg tgacatgata aagccggagg aaacttgcct ccggcattac tatttacttg
     2401 cctgtgaccg ctgctgccgt tcctggcacc atcagctctg tagcaacgat aacaatcacc
     2461 aaaccaacaa gcaccggtac cgaggtgcgt tttacgactt caaacggcga gatcttcgcc
     2521 atcccggcaa ccgcaacgac tacgccagaa acgggcgaaa gggtacggcc caggtttgac
     2581 gcctgcagca tcgggatagt caaatacgcc gggttaatgc cggaagagtg cgccagtttc
     2641 gggatcatct caacaaacgc ataaaacggc gcattgcctg aaccggtcgt gactgccgcc
     2701 agcatggtca gaatcaccaa taccagcatc aggatgatac tcgccgaacc aaacgaggta
     2761 gcgatagaaa tcagactttg aataaagccg atggtgctaa gcccctgagc gaatacccca
     2821 gcggcaacca gcagcatcac cacgttagca aacgcatctg ccattccgcg ataagccact
     2881 tccagaccag agaaaacttt ctgggtatta aagctgcgga ggaactccag aatggaggca
     2941 atcagcatac aaatcaccag aatagtgatg atgtgtaatt gcggacccca tttaccgtca
     3001 aaaatcagta caccgatgat cggcgtgaac ggcaaaatgg cataaaacgc aggagcagtg
     3061 gtggtgattt cactgacatc taacatttca tgagagatgt gctctttttt atccagataa
     3121 cgttgccaga agaagtgggc gatcgccatg ccgataattg cagcaattga gataggcagc
     3181 gtcgttttga aggcgaagtc aatcagcgac atttcggaag cttgcgccgc cagcaccaca
     3241 tcccctgaag tcggtgcgag aataatcgcc gccggggagg cacaaatggc agcagctgcg
     3301 ccacgactga taccaacgtt taccatcacc ggaaataggg ttgccatcag caaaacaccc
     3361 agaccggttg cggaagagac ggccagagac atcagacagg cgacaaaata ggcggcaatc
     3421 atcagcaggt aaggggagtt aatatactgc aatggttttg acgccagctt gaccaccata
     3481 tcattcgcgc cgatatgggt catgtaagcg gcaaatccac acagcatcat aatcatcatg
     3541 ccgaggtcgc cgccgcggct cattagtaat attttaacgt attcaacgat atccgtggcg
     3601 ctgtagcctg ttgaagcctg gctggacggt aacactttgt gccccataat ggcactgata
     3661 atcagcaata acaggccacc gacaaataac acaccagtgg cggaataccc tttaatgatg
     3721 tagcgagcta cacccacaat aaccacaacc ccaataagga gttcaatgaa tgtcagcata
     3781 atttttcctg tctccaggcc ccaaagtaaa taataaaaaa ttcctaaagc ttaaggaaaa
     3841 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     3901 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     3961 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     4021 gttagctgcg gctgtgcgct caaaaaataa tctaacatga gcataggttg acgatttata
     4081 tta
//
LOCUS       DAZFDP010000277         4082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFDP010000277 DAZFDP010000000
VERSION     DAZFDP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHJ1G_005094"
     CDS             complement(<1..67)
                     /locus_tag="ACHJ1G_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766769.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHJ1G_005095"
     CDS             complement(64..639)
                     /locus_tag="ACHJ1G_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766770.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHJ1G_005096"
     CDS             complement(654..1007)
                     /locus_tag="ACHJ1G_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4766771.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHJ1G_005097"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHJ1G_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4766772.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..2310)
                     /locus_tag="ACHJ1G_005098"
     CDS             complement(1426..2310)
                     /locus_tag="ACHJ1G_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4766773.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVASSRYPKHWLTVGDPAREFTMTQS
                     APLMVLPDPDEFVVVQVK"
     gene            complement(2368..2715)
                     /locus_tag="ACHJ1G_005099"
     CDS             complement(2368..2715)
                     /locus_tag="ACHJ1G_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4766774.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(2752..>4082)
                     /locus_tag="ACHJ1G_005100"
     CDS             complement(2752..>4082)
                     /locus_tag="ACHJ1G_005100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4766775.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          863 a         1290 c          982 g          947 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcaccgc
     1621 tgtttcgagt tgcgaattag agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga cggtaccgtc cataatggca atattcacaa gaccgctggc
     1741 ctggtcgcag tagaggtcaa gatcatgtgt aggatcgaac gtgtcacgat cctgttttga
     1801 ccactcctta ccactaccct gagtgatgtt attcttcgtc gacctgccaa aatcgacctc
     1861 aattttctcg aactggtctc cttccatcgt gtatttgcca tacagcacgg cattcaccgc
     1921 ctgcatttct tccacccgga caatggcgtg ctcttcctgt ttgaggttat cagtgatgat
     1981 acgcagacga cggtaggccg ggtcgttcag ctgagccgga tcttcaccgg gaagacgctc
     2041 aaccgcctgc tggtaattaa attcgtgttt cggcttgacg tagcccggac gtaacacgcg
     2101 ggtttcacca ccacgatgac gcagcacttt tccttcaaca accggggaaa cataggccgc
     2161 caccggcgtt tttccggtaa ttttgtccag catcacctct tcggtatgga aattcaccgt
     2221 acggcggaaa aacagctcca gaaacagcgc acgaaatttc actttttgtt cggtataacc
     2281 gagtaactgg cgggtcgtaa acaatcccat aaatcagttc ctttcattca gaaatcagtc
     2341 aggccaccgc ggtggcctga taacgtgtta cggcagcgcc gcgtgactca gggcactgcc
     2401 ggcaaaggcg ttggcctttt tgtgttcatc cacactttca ggccagcgga ttgcctccgt
     2461 cgcaaaggtc cccgacttgt aataggtcag cgccgtctct gtgccttcaa gcggcagtac
     2521 cagtatgcca accgcactac cggctttctg tccgtcccag accaccagtt tcccggtggc
     2581 ctcatccagc atcaggggcg tcagtgccgg tgttgccgag gaaatcccgc tgctgcctgt
     2641 ggcggtatga gccggatcat taccggcaaa aatacgtact tccacacgct gttcagtgat
     2701 agttttcgtc accatattgt aaaaacctcc tgttgatggt cagcactggc ttcatggcat
     2761 ggccatgagc attttcacgt ccgcatcacc gtctgctgac gtctgtggca cgccaccctg
     2821 taccgctgcc ggtgaatggt tcgccatgat gcgttcaaac atggcggttg tggatgcaga
     2881 gaccggttct gccttacctg atcccgcagc cagcacagcc cgggcgctct ccacagtcat
     2941 tcccgggcag gcagccagct gttcagcctg cgcctcagcc ccttttgcct catccagtgc
     3001 catgatctga tcacggagtg agggtccggc atccgcctgc ggtgaagcag ccaggatcgg
     3061 gcgggctttt tccaccgtca tctccggcat cgccgccagc gttgccgcca gttgttcacg
     3121 acctttcgct tcttcacacg ccataatgcg atcggcttca ctctgcgcgg atgccaccgg
     3181 ctgctgtggt gccgccgcgg ccagaatcgc ccgggcctgt tcaacgctca tgccctgttg
     3241 tcctgccagc atcgtggcaa gctgttcacg tcctttcgct tcctggcatg tcaggatccc
     3301 catcactcgc tggttctcct gcgcggcggc ttccgttgca gttaattgcg gcatagtgcc
     3361 tcctctgaca ttactgttca gcgccgtggc catcacactg atggcatccg acgcattgat
     3421 taattcatcc gccagcccgg cctcaatgcc ggactgacct tcaaaaacgg cggcctctgt
     3481 tcccgtgaca gcatcaacag acagaccggt atacatcgct actttttcag caaacatccg
     3541 gcgcgccgca tcaatgcgct gttgcatgtc ctggcgaacc tctgccggca acgcttcaaa
     3601 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcaa caccggcctg
     3661 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
     3721 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagagcaggc
     3781 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
     3841 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtacgccccg
     3901 cacctggcta tccgccattg cctgctgaag acaggcgaca atgccgtcat agcctgtcat
     3961 tccggaaaat ggccgcatac cccccagccg gtgcaccagc gtgccggtca ccggcagtac
     4021 agcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
     4081 gt
//
LOCUS       DAZFDP010000278         4012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFDP010000278 DAZFDP010000000
VERSION     DAZFDP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..287)
                     /locus_tag="ACHJ1G_005101"
     CDS             complement(<1..287)
                     /locus_tag="ACHJ1G_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766776.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVT"
     gene            complement(280..3546)
                     /locus_tag="ACHJ1G_005102"
     CDS             complement(280..3546)
                     /locus_tag="ACHJ1G_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766777.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMAAGVGKATTELIAAKSGTVA
                     LAAAQADAAVQVQRKAAADALAAERAWQLAQAELALARNSSAEALATQNAVAKRRAMI
                     VANAALVQSNQAVAASRKALENATSVTGMLKSGAASLLSLVGGIPGLLMLGAGAWYTV
                     YQNQEQARESARQYASTIDEIRAKVPGMTLPELADNEGKTREALEEQNRLIAEQESKV
                     RGLKTQIADYQRWLQENPQGGSGVEIITHGLAEATGKLAVEQSRLTQMQEKAKSVQEA
                     LASLEYNRVARIREEAAEQNRAYQALLQMNVQHTEFNRLLGLGNELLQQRQGLVNVPL
                     RLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRY
                     ISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQ
                     TAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGY
                     GQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKE
                     QEAFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNG
                     NKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAK
                     GGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGS
                     MNGSAGLVYSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG
                     "
     gene            complement(3598..3807)
                     /locus_tag="ACHJ1G_005103"
     CDS             complement(3598..3807)
                     /locus_tag="ACHJ1G_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4766778.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVAPFAALEDALSLAAMS"
     gene            complement(3903..>4012)
                     /locus_tag="ACHJ1G_005104"
     CDS             complement(3903..>4012)
                     /locus_tag="ACHJ1G_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4766779.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          766 a         1370 c          935 g          941 t
ORIGIN      
        1 gtgacgctcc actcccggca caccaccctc accgtccggt tatgtttcgg cggtcgccac
       61 aaaaaggcac ggtaaccacc atgccaggat aaaaattcat ccagccagcg ccgggttgac
      121 tcatccgtca cccggaacac cgcctgaaac gtcttcagtt gaggattcag ccctgtgggg
      181 cggcgctgtt cataaccgtc accaaaccgc accctcacca ccgacggctt ctcactcacc
      241 tgcatccctt cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc
      301 gtcacgtcgc atggacagca tcaccgcctg cacccgctgg tcaatcagct gcacaaggct
      361 gcccgcagct tccggcccta tctgtccgtt agccccgtca ttctgaatgg cgatgtggta
      421 gaccggggaa tacaccagac ccgcactgcc gttcatacta cccaccgcgc gcacacccag
      481 cgagccatcc gccgcccgcg tcagaggcat aatggcttca ggtccggcct cccccatcag
      541 cccggcccct tttgcaaagg caaagtacgt gggcgtatcc acaatactgt tgctgtacgc
      601 actcaggttt gccgaggtat acacgccgcc ttttgcattg gccaccgctc cgcccagcca
      661 gtcaccaatg ctgccgagaa atcctcccgc accggacata ccgtttgccg ccgtcttaat
      721 tccgttgaca atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc
      781 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
      841 tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg taatcggacg cattatccac
      901 ccagttactg aatccctcct gcagcccttt ctgccagtcc gcacgctgcg catccgattc
      961 ggcataaaag gcttcctgct ctttcagacg ttcactcaga tactgtgcat tctgcgccag
     1021 agcctgtctg taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc
     1081 atccttctgg cggaagctgt cgcggatctg ctgcaactcc cgcatgcgtt ctctggctcg
     1141 ttccccctgc ccgtacccca gcagttcagc atcattcgac gcacgcgcag ccgcattctc
     1201 attcttcagt gtctcttccc gggatcgcaa ctgttcccgg attttttgct ggtcaatcag
     1261 ggccgcatta cgcagcagct cctgcttctg catctccgtc agggttttca gttcgccctg
     1321 cgcagtctgg tatttcagct tcgccagctc tgtattctgc cccaccagtg ccagttgctc
     1381 tttctgctgc ttcagcagcc gggaaaaact gtcttccgct ttttccgtct ctgattttcc
     1441 accccgggat ttgggtttat tcgcctcgtt attgcgccag gcttccaggg cattactgat
     1501 ataacgttgt ctcgcctcct gatacggatc acccacaaaa ccgaggtcat ccgccgcata
     1561 ccccagccgg acacgctctt tttcttcccc tttcagtctg gacagggcca gctcacgctc
     1621 tgtttttgtc agggcactct gctgtttatc atccagggtg gcctgtggca gccgtaacgg
     1681 cacattcacc agtccctgcc gctgctgaag cagttcattc cccagcccca gcagacggtt
     1741 aaattccgta tgctgaacat tcatctgcag caatgcctgg tatgctctgt tctgctctgc
     1801 tgcctcctcg cgtatcctcg ccacacggtt gtattccagt gacgccagag cttcctgtac
     1861 agacttcgcc ttttcctgca tctgtgtcag tcgtgactgt tcaaccgcca gtttgccagt
     1921 tgcctccgca aggccgtggg ttataatctc cacacctgaa ccaccctgtg gattttcctg
     1981 cagccagcgc tgatagtcag caatctgtgt tttcagtccc cggactttac tttcctgctc
     2041 agcaatcaga cgattctgct cttccagtgc ctcgcgagtt ttaccctcat tatcagccag
     2101 ttccggaagg gtcattcccg gtacctttgc ccggatttca tcaatcgtcg atgcatactg
     2161 acgggcggac tccctggcct gttcctgatt ctggtacacc gtgtaccagg caccggctcc
     2221 cagcatcagt aaaccgggta tcccgccgac aagcgaaagc agggatgccg cgccactttt
     2281 cagcattccc gtaaccgacg tggcattctc cagcgccttc ctcgaggccg ccaccgcctg
     2341 attcgactgt accagtgcgg cattggccac aatcatggcc cggcgttttg cgacggcatt
     2401 ctgtgtggcc agcgcctccg cactgctatt tctggccagt gcaagttcag cctgtgccag
     2461 ttgccaggca cgttctgccg ccagcgcgtc agctgccgcc ttacgctgca cctgaacagc
     2521 cgcatctgcc tgtgcggctg caagggcaac cgtcccggac ttcgccgcga tcagctctgt
     2581 ggtggccttt cccacgcctg cggccatatt gccaaagtac cgggccaccc cgacggcaac
     2641 cagcgccccc gcggctgttg ccacattatc aatattaccg gcaacaccgt tcagcacgcc
     2701 ggagagcgtt ttcgtcgctc cgctggcttc attcgcgcca cccacccagg ccataaaggc
     2761 gttttccacc tttgtgatcc cgtcagagac cgtttccggc atggcggcat attcatcacg
     2821 caataccccc agctggctga ttaacgcagg aacgacttta tccgccgtca gtttgccgtc
     2881 gtccgccatc gccttaaggt ctttacgggc cacgcccata cccgcagcca gtgcacgtat
     2941 gatccggtct ccgctttcat tgaccgaatt aaattcctca ccccgtaaca caccctgtgc
     3001 cagcgcctgg ctgaactggg tgatcaccga gcccgcctct gccgtactgg caccggatat
     3061 tttcagccct gtcgaaatgg cctccgtcac cttcagtaca tcaccggcac tgtaaccata
     3121 ttcacgcatc gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgccgtgcc
     3181 tgtccgctgg ctgatatcca tcagcacttt ctgtgatgac gaaaattcat cagatgactg
     3241 cgacgcctgt ttcagacggg cattcacgga actccactca tcagccagtg aaatcaggtg
     3301 tccggtggca aaggcaccgg caaatgcgcc agccattccg acagcagaac cgcgaatttc
     3361 cgtcaactgg ctgttcagtt ctgccagggc acgtcgctgc tcccgggctg ccgcagcggc
     3421 ctgacgcccg ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg
     3481 gatctccgac tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt
     3541 cgccattcac ctttctccgg gcaaaaaaaa cctgccacag caggttttca tcattattta
     3601 tgacattgct gcaaggctca gcgcgtcttc cagcgccgca aacggagcca cctccggctt
     3661 atcctcatcc tcgccccagc agagcatggc gtccttcagt gcaacattca tcccctgtgc
     3721 cccgaaaacc gctttcacga tctgtgcatt acggatatcc ccgcgctcat cacccagcgg
     3781 ggataccctg tcgaactcca tccacatcat cgcctcgctc gcactcaggc tgtgccgcag
     3841 ttcggataag gtgcgcccca gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg
     3901 ggctactttt ttctggccga ctctgcatca gcgatcagtt ccagtgcctg acgcagcaac
     3961 cgggcatgta ccggaccata gacggccagc acctgctcac ggtcgtccgg ag
//
LOCUS       DAZFDP010000279         3940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFDP010000279 DAZFDP010000000
VERSION     DAZFDP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..189
                     /locus_tag="ACHJ1G_005105"
     CDS             <1..189
                     /locus_tag="ACHJ1G_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4766780.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKAARGE"
     gene            194..538
                     /locus_tag="ACHJ1G_005106"
     CDS             194..538
                     /locus_tag="ACHJ1G_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4766781.1"
                     /translation="MNKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPYVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            589..1122
                     /locus_tag="ACHJ1G_005107"
     CDS             589..1122
                     /locus_tag="ACHJ1G_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766782.1"
                     /translation="MNAKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVLPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1415..1546
                     /locus_tag="ACHJ1G_005108"
     CDS             1415..1546
                     /locus_tag="ACHJ1G_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766783.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            1510..1665
                     /locus_tag="ACHJ1G_005109"
     CDS             1510..1665
                     /locus_tag="ACHJ1G_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766784.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            1677..1763
                     /locus_tag="ACHJ1G_005110"
     CDS             1677..1763
                     /locus_tag="ACHJ1G_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766785.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            1985..2170
                     /locus_tag="ACHJ1G_005111"
     CDS             1985..2170
                     /locus_tag="ACHJ1G_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4766786.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2571..2897
                     /locus_tag="ACHJ1G_005112"
     CDS             2571..2897
                     /locus_tag="ACHJ1G_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO4766787.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(3029..3229)
                     /locus_tag="ACHJ1G_005113"
     CDS             complement(3029..3229)
                     /locus_tag="ACHJ1G_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4766788.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3271..3636
                     /locus_tag="ACHJ1G_005114"
     CDS             3271..3636
                     /locus_tag="ACHJ1G_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4766789.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAASDMPD
                     PTLPEVSGGSGREDDIIGL"
BASE COUNT          945 a          848 c         1129 g         1018 t
ORIGIN      
        1 ggtgtgtcat acaccacgtc agcggtgggg acgggatact ggttactgca gctgctggac
       61 aaagtctctc cgtcccagtg ggtggcgata ggtgtgctgg ggagtctgct gtttggcctg
      121 ctgacgtatc tgactaacct gtatttcaaa atcagagagg accggcgtaa ggcggcgcgg
      181 ggagagtaaa gtgatgaata aaaaatacga actggttgtt aaagggataa ataattaccc
      241 ggataagatt actgttactg tggcactgga aattggtggg tatccgtcac tgttgttgcc
      301 atatgtggcg attagtcttg accgtactga aggtgccacg ctggagtttt acgaagctga
      361 ggcgaaaaag caggcgaagc agtttttcat ggatgttgct gccgggttat gtgaagggga
      421 tggtccgttg ccggaaaagc ggcccatcat tttagaggcg caggatgtgt tgataaccta
      481 cagaggaaaa ctaccgggaa taattactgg ttctctgaag actccaccgc tggcctgaag
      541 acttaacata tccagggatt tgaaatcgat aaatcctgat aaatatccat gaacgcaaaa
      601 atcaaatacg gcctgtcggc tgccgttctg gcgctgattg ccgcaggtgc gcctgcgcct
      661 gaaatcctcg accagtttct ggatgaaaag gaaggtaacc acaccacggc ataccgtgat
      721 ggcgcgggta tctggaccat ctgtcgcggt gccacccggg tggatggtaa gcctgtcctc
      781 ccgggcatga agttgtcgaa ggaaaaatgc gaccaggtta acgccattga acgtgataag
      841 gcgctggcat gggtggagaa aaacatcaaa gtgccactga ccgaacccca gaaagcgggg
      901 atcgcgtcat tctgtccgta caacattggt cccggtaagt gtttcccgtc gacgttttac
      961 agacgaatta atgcaggtga tcgcagggga gcgtgtgagg cgattcgctg gtggattaag
     1021 gacggtggca gagactgccg tattcgttca aacaactgct acggtcaggt atcccgtcgt
     1081 gaccaggaga gcgcgctggc gtgctggggt atcgacagat aagcagaata ttttgctgaa
     1141 aaataaggca tggccacgcg ggcggataac acgaaatcct gcgaactggc gaaacgtaag
     1201 tgaataaaag taaaaatccc gtttgttggc agcaagcggg gttttgtttt tatggcagta
     1261 agctatggga ggctgccttg attgatttta gcaaactgat tagggagttg cgactcatga
     1321 ttagtcaatt accaaactgg aaatttttgc tggtctggag catccctttt ttatgggtag
     1381 tatcccagtt aattgtggca attaaggggt agctatgtca gacaaactca taacgccggc
     1441 aaaggtcctc tgtgtgattg tcggtatttc attttcacta atgctggttg ctctttttct
     1501 gtccctcgcc tgggtgatgt tgtcttcgtc ggggctgctg gggtgacagt gactgatgac
     1561 atcagcagag cgctggcttt tgctattaag tgggtggctg ttggtattgc tgtgtctccg
     1621 atgctatatg ggctggcaaa actggtcatt gcgctgaaat cgtgaacttt aaaaagatga
     1681 gtgctgaact tattcgggca atggcatttg ccattcgtat tgtggccatt gctgttctgg
     1741 tctgggcaat ccgttggtgg tgaaatgaac cgcgttctgt gtgtggtcat cattgctctg
     1801 ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt accgcgataa cgccatcact
     1861 tacaaagcgc agcgcgataa aaaagccagt gaactgaagc tggcgaacgc aaccattact
     1921 gatatgcagg tgcgccagcg cgatgttgct gcgctcgatg caaaatactc gagggaatta
     1981 gccgatgcga gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc
     2041 ctgcggatca acgccacctg ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg
     2101 gataatgcaa ccggcccccg actggcagac accgctgaac gggattattt catcctcaga
     2161 gaacggttga tgacaatgca gaagcagctg gaaggggcgc aggaatatat ccgtactcag
     2221 tgcctgaaat aagttttgtt gatgcgccgt atcgtcgctg tattccctca ttaacagaga
     2281 ccgcagcccg acagggagac tcctctgcgc gagtgtgcgg ggataatcaa aaacgataca
     2341 caccggggtt taccgcgtta acggagcgcg gcgttgtccc ctcatagtcg ccagtccggt
     2401 gcgatggtgg aagaaacagg acgatgtgtt acctcgcaag ccctgttatg tcatgtgtct
     2461 gatttgtgat ttaagtcgga taattgtcgt tgccattaag cagaggattg atgaccgaca
     2521 gggcggcatt gttagaataa gacttatttt tatctgtgcg gggaatgaaa atgaaaagaa
     2581 atcttccgtt aattattttg ttgtcttctc tggttatggg ctgtacgcaa cataaaacag
     2641 atatgccccg acagttggtt aaggcattac cacaatatcc ggcctatgca gcggcaaatt
     2701 atataaaggg acgggttgat gtgaggtttg atattggtgc tgatggtact gtcacccgaa
     2761 ttgagtttat tcgttcagag ccgcaccatc tgtttgatga gcaggttgta aaggcgatgg
     2821 caaaatggcg atttgagaag gacaggccgt gtaaaggcgt gaagaaaacg tttatcttta
     2881 gtccttctgc accctgatta tttcatcaga aattaattat cactctgttg ttattctgta
     2941 catcccggca gggtaagtct tgttccgtcg gatatgaaga tgaaatattg tgggaggaca
     3001 gtgggtacct gctcctgtaa ccgaacgttc atttctcgtt atttgtcatg ctggccgggc
     3061 gcagatgcgt tgcatctgtt gccagccttc tcctgcaggc ttcaataacc cacgctgaaa
     3121 agttaccgga acctttatgt tcaagggcga tattgatctg ttcaatcatg tgattgggga
     3181 aacggatgtt gcgggttgtt gttctgcggg gccggttttt cgatgacatt ttctttcctc
     3241 tggtgacaag ctatatggag aggattttac atggctgtgc ttcgtacgtt accgggcaga
     3301 atcaaaacac tgaatacccg acgggtgaat attctgaagg gtgaacagcg tcgtgtcagt
     3361 ggcagtgcac gtgtttccct caagcgtcat atctggctca gggacgccgg gcagtgccgt
     3421 ctctgtggtc gtgtggttga cctctgtgac agtgaactcg atcaccgaat tgcacttcag
     3481 ttcggtggtg gtaatgagga gacgaatctc tggacgctct gtaccgaatg ccatcgacaa
     3541 aagtctgctc gtgaagcggc gagtgatatg ccggacccga cgctgccgga ggtgtccgga
     3601 ggtagtggca gagaggacga catcatcgga ctgtaacccg accccggggg gggtatcatc
     3661 cggcgtaaaa aacgatcgct ttggacaccg ccccccgtct cacgcagaga aaaaattccc
     3721 gtttcaggcc agttaacatg ttaactggct gtccgggcat ttttgcggtt tttatcttta
     3781 ttattcagtt tgttgcgtgg aaaaaatgtt aacaggcttt ttcagcaaat gttaaccagg
     3841 cagcagttaa catttgcggc atgagacgcc gggaaaaatg ggctgaacca tacccggctg
     3901 agtgcgttat ggacccggga ggaggctgtg ctgacaacgc
//
LOCUS       DAZFDP010000280         3929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFDP010000280 DAZFDP010000000
VERSION     DAZFDP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1447)
                     /gene="rhsD"
                     /locus_tag="ACHJ1G_005115"
     CDS             complement(<1..1447)
                     /gene="rhsD"
                     /locus_tag="ACHJ1G_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO4766790.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDT
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
     gene            complement(1515..3623)
                     /locus_tag="ACHJ1G_005116"
     CDS             complement(1515..3623)
                     /locus_tag="ACHJ1G_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4766791.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKPPDEKQDTPDMREY"
BASE COUNT          766 a         1226 c         1052 g          885 t
ORIGIN      
        1 acaccaccgc cgtcagctgg ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg
       61 gcgtggtgat gtccgtgata tcgcctgaca ccacattcag accgtactct gtccgccgtc
      121 ccgccgcgtc cgtctgcgcc gtgagccttc ctgccgcgtc atacccgctg tgcgtgacgc
      181 tgccgtccgc cagttctttt ttcaccaccc gcttcagccc ggccccgcct tctgtatgca
      241 gcacctcacg ccggttcagg ctgtccgtga cggtgatgcg atcctgctca tactggtagc
      301 ggtaactcag gcctgccggg ttaagttgct ccaccacccg ccccgcatcg tcgtagcggt
      361 agcgcatctc cggcctgcct gcataacggt gcgccaccat ccggcccggg tgctgcgcgt
      421 catacgtgaa agcgcgcacc tgcgtattgc tgcggtcata taccgccagc agttcaccgg
      481 cttccgtata tgtgtaccgc accagtggcg cagcgggcag gctctccggg tatgccgggt
      541 cgtgcatcag ccacaccgcc gaaaggcgga tacccctgtc ggggccgtat tcggtaccgg
      601 gcagtgtgtc ggggaacgct gaggctgaga gagggcggga actgtcagaa gaagatagcg
      661 aagaggtgcg ggcctcttcc gcacgctgcg cctgcgtggt cagcaccaga cggaactccc
      721 gcccggcacc gtccgtcacg ccggtgattt ccccggtcag gtcaccggcg gcctcacgcc
      781 ggtacgtcag cgtccgcccg aagcggtccg ccagccccgt cagctcccgg tacggcggca
      841 gcggcgctgg cagcacgtcc tcagcacccg gcacccgctc tgaccagccg agtatccacc
      901 acggcccctg tgcgctgttg gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg
      961 gcagcgcccc ccacagccgc gccagcgtat ggccgtccgg ctgtgctgcc ttaccaccgc
     1021 gcaccagcca cattgactca ctgcggctgt acaccgcctc ccccggcagc agcggctcaa
     1081 agtgaatgct ccgcccgccg ttgtcgttga gtatcagtcc gtcatcacgt agctgtaagc
     1141 gggtatcaga aggcgctttc cagccggggc cgaaaacgcc caccggtgca ggcgtcttcg
     1201 tccggtagct gctgtaggtg cgggagagaa tgaacggcag cgggccgggc agcgcaaggt
     1261 ccgtctcgcc gggcagcacc ttcgccccca gcagcggatt taccgggttg cccgaagtca
     1321 tcccgcccgg acacaccgag cacgccacgc cggtgggcgc gccaattctt acacctgccg
     1381 aaccctggac aatgggaccg ccatactgag tcatatctcc ctgacgcgcc gctggttttc
     1441 cgctcatgat tcaaattcct tcttctgttt atttttaatg ctggtttgtt actgaattcc
     1501 gaacaggtac gggatcagta ctctctcata tccggcgtat cctgcttttc atcagggggc
     1561 ttcggtgggg atttcgaggc tgcacagttc cagttaatca acagtgagtc accattaacc
     1621 tgctccttac cctgcaaatg aatttttgtg ccgttgagaa aaatttgtcc gtccttcgtc
     1681 agcaccagcc ttgccttacc acagcagagc tccagatgct caccggcgga gtaaatcgcg
     1741 gtctgcccgg tatcatcctt tttcgtttta ccaacggtga aggtaacgtt atttccgact
     1801 ttgacgtcat aacccagccc gaccctcaac gatttatgca aaccgatctg cgaggactgc
     1861 atcagagcca ccgaagtgtt cataatgcca cctaccgtcg tctggtacgc cacgcccacg
     1921 gtcagcgcac gaacaagtcc aatcgtttcc acctgaacag aacccacgtt aatgatctgg
     1981 ttactgccca ccgtctctat ctggttaacg cccaccgtct gtatctgatt gttggtaacc
     2041 gcaatcatct gattgttgcc aatggtctca gcatggttgt ttatcacatc cgtggtgcgg
     2101 ttattcagca cctcggtgtt catgttcttc tgcgcgtgga tgtagacctg ttctttccct
     2161 gtcgcatcgt caaacttcag ttcattaaac ccgctgccct tataggtttt cgagcggatc
     2221 gtcatctgcg tcttcgtccc cggcaggctg ccgggtgtgc ggttttcatg gtggtaggta
     2281 cgccccataa taatcggctg gtccgggtcg ccgttgagga agtccacaat cacctcctga
     2341 cccacacgcg gtatcgccag gtggccaaaa ccggtgcctg cccacgcctg tgccacacgg
     2401 atccagcacg aactgtcttg gtctgccggg ttataacggt cccagttgaa cttcacccgc
     2461 acgcgaccat gttcgtcgca gaagatttcc tcgcctgccg gtcctgtcac gacagcgctc
     2521 tgcgggccgt cgaccagcgg tttcagcaac ggctgtggtc gccatgttct gtctgccggg
     2581 atcaccgcaa aatggttctc cagcgccgtc cccgctccct gccgtcctgg caccgcctgt
     2641 ggctgttcgc cgtgcagttc acttgccacc acctgccatt cccggttcag gttcgcctgc
     2701 ggatgccccg tcagcacaat tcgtcgtccc ggccatatct ccggcgagcg actcattccc
     2761 cgcgcggttt ctgcattgtt tcgccagccg tccatctgcc agcgggcaaa gttctgcccg
     2821 tgggccccct tgaaacgtcc ggggtagtca tacacttcat actgtgtgcg ctggtaatcc
     2881 tggtgctggc cttcctgatc aaaacgtccg gcccagccgg ggcgtttaaa ggtgtagtct
     2941 ttggtcacca cggaagaagg gcggatttgt gcgctgtagc ggaactggct gatgcagagg
     3001 gtgctcacct cggtacgggt gttcgggttc catgggattt caaaagattc gggcagatgg
     3061 cggactgtgt cgcacagcac caggctctgg tcggtacttt tgtaagcatg ctcctcataa
     3121 aagaagatgc cttcctccgc cgccatccgg cacaggaaat cgtaatcagt ctcaccgtac
     3181 tggacacaaa actcacggga aggatgcggc tcgctgaaca gcggactcca ttcggtcacc
     3241 ccgttttcct gcaacatcgt gccgaggatg cttttgatgt cctcgttctg gaagatacgg
     3301 aagttctggc gcagaccggc acgccacagc ggcgggtgca ccttcatgct gtacagcatc
     3361 tggtttttgt cgttctcccc gagttcaaac caggtcacca cgcctttcac ccggcgctgt
     3421 acttcatcgc cctgccatat cgtcaggtag gccattttgt ccagcacctg cgcaaattca
     3481 agggagagaa actgctggct gaccagggag agatcgaggg aaaagagcga agagagtgac
     3541 tggttcagat gaaaggaaac caccgcaaaa gcatccggcg gcaggccgtc cacttccagt
     3601 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
     3661 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
     3721 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
     3781 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
     3841 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
     3901 atattcataa tgtcttttta ttcacaaca
//
LOCUS       DAZFDP010000281         3906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFDP010000281 DAZFDP010000000
VERSION     DAZFDP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHJ1G_005117"
     CDS             <1..450
                     /locus_tag="ACHJ1G_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4766792.1"
                     /translation="DIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHRE
                     DDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHH
                     LPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            447..1028
                     /locus_tag="ACHJ1G_005118"
     CDS             447..1028
                     /locus_tag="ACHJ1G_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4766793.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1371..3815
                     /locus_tag="ACHJ1G_005119"
                     /pseudo
     CDS             1371..3815
                     /locus_tag="ACHJ1G_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
BASE COUNT          871 a         1034 c         1213 g          788 t
ORIGIN      
        1 gacattcaca aattccgctg tgtgccacat ctgacaggac ggcgctttga gcacggggtg
       61 acggactgtt acacgctgtt ccgggatgct tatcatctgg cggggactga aatgccggat
      121 tttcatcgcg aggatgactg gtggcgcaac ggtcagaacc tttacctgga caatatggcg
      181 gtcaccggct tttaccgggt gcccctgtcc tctgcacagg cgggcgatat tctgctgtgc
      241 tgctttggtg cttcggtacc gaaccatgcc gccatttact gcggcaacgg tgagctgctt
      301 caccatctgc ctgaacaact gagtaaacgg gagaggtatt ccgaaaaatg gcaacgacga
      361 acgcattctg tctggcgtca ccgccactgg cacgcatctg ccttcacggg gatttacaac
      421 gatttggccg ccgcctcagc ctgtatgtga acacggcagc ggaagccatc cgggcgctgt
      481 cgttacaggt gccgggcttt cgccgtcaga tgaacgaagg ctggtaccag atacgtattg
      541 ccggtgatga cacggcaccg gaggcggtgt acgcccgtct tcacgaacag ctgggtgagg
      601 gaacggtcat ccatattgtg ccgcgactgg ccggggccgg aaagggtgga ctgcagattg
      661 tgctgggggc ggcagccatc gtgggctctt tcttcactgc cggggcatca atggcgttat
      721 ggggttcagc cctggcagcc ggtggttttt ctgccaccac aatgctgttt tcacttggag
      781 ccagcatgat tctgggcggt gtggcccaga tgctggcccc gaaggcaaaa acaccggatt
      841 accgcgcaac ggataacggc agacagaaca cgtacttttc ctcgctggat aacatgattg
      901 cccaggggaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc cgccgtatat
      961 cccaggacat cagcacccgt gatgaaggcg ggggcggaac ggtcgtggtt gtcgggcgac
     1021 agggataaaa cataaaaaaa tcccgcagtg atcgcggagc tgcggggaca gacaaatgaa
     1081 gatcaatgtt aaggagttgt ttttgttact cgggcaaaaa aacactaacg cagcgaaatt
     1141 ataagcgcca cagtcagtgt gtgaaaatgt gaagatattc agaattttta tgccattacc
     1201 ggttttaacc aacaggatta tcggtgggca tgaaagaaaa ccccggtatc tgctgatacc
     1261 ggggtttctc tttagcatgg cagaaatgtg tttcatgctt ttcgggcgaa ggatatccga
     1321 cttctgtacg gaatggcaag tggcggttaa tttattcagg ggaaggctgt atgggaaaag
     1381 gtggcggtaa ggcacacacg cctcgtgagg cgaaggataa tctcaaatcc acgcagatga
     1441 tgagtgtgat tgatgcgatt ggtgagggac cgatagaagg tccggtgaag ggactgcaga
     1501 gtattctggt gaacaaaacc ccactgacgg acacggacgg caatcccgtg atacacggtg
     1561 tgaccgcggt ctggcgtgcc ggggagcagg agcagacacc accggaaggc tttgagtcct
     1621 ccggagctga aaccggactg ggcgtggaag tgacgaaggc aaaaccggtg acgcgcacca
     1681 ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca ctggtggaga
     1741 ccacctcaaa gggtgaccgt aacccggcat ccgtccgcct gctgattcag ttacagcgta
     1801 acggtaactg ggtgacagaa aaggacgtca ccattaacgg caagaccacc tcacagttcc
     1861 tggcctcggt gattctggat aatctgcctc cccggtcctt taacatccgg atggtcaggg
     1921 agacggcgga cagcaccacg gaccagctgc agaataagac gctgtggtcg tcatacaccg
     1981 aaatcatcga tgtgaaacag tgctacccga acacggccat tgtggggctg caggtggatg
     2041 cggagcagtt cggcggccag cagatgacgg tgaactacca tatccgcggt cgcatcatcc
     2101 aggtgccgtc aaactatgac ccggaaaaac gcacgtacag tggtatctgg gacggcagtc
     2161 tgaaaccggc atacagcaac aacccggcct ggtgtctgtg ggacatgctg actcacccgc
     2221 gctacggcat gggaaaacgt ctgggggcgg cggatgtgga caagtgggcg ctgtatgcca
     2281 tcgggcagta ctgcgaccag acggtcccgg atggtttcgg ggggaccgag ccgcggatga
     2341 cctttaatgc gtacctggca caacagcgta aggcgtggga cgttctcagt gatttctgct
     2401 ctgcgatgcg ctgtatgccg gtatggaacg gtcagacgct gacgttcgtt caggaccgcc
     2461 cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg
     2521 gattccgcta cagcttcagt gccctgaagg accggcacac ggcggtggag gtgaattaca
     2581 ccgacccgca gaacggctgg cagacctcca cggaactggt ggaagacccg gaagccatac
     2641 tgcgctacgg acgcaacctg ctgaagatgg acgcgttcgg ctgtaccagc cgcggtcagg
     2701 cccaccgtgc cggactgtgg gtgataaaga ccgaactgct ggaaacgcag acggtggatt
     2761 tcacgctcgg gtctcagggg ctgcggcaca cacccggtga cattattgaa atctgtgata
     2821 atgactatgc cgggaccctg accggcggac gtgtcctgtc cattgatgct gccacccgca
     2881 ccctgacgct ggaccgtgag gttaccctgc cggagacagg tacatcggcg gtgaacctga
     2941 ttaacggcag cggtaagccg gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga
     3001 tacaggtcag taccctgcct gatggtgtgg agacatacgg ggtgtgggga ctctccctgc
     3061 cgtcactgcg ccgtcgcctg ttccgctgtg tctccgtccg ggaaaacacg gacggcacct
     3121 ttgccatcac ggcggtgcag cacgtaccgg aaaaagaagc catcgtggat aacggtgccc
     3181 gctttgagcc gcagtcaggt tccctgaaca gcgtcatccc accggcagta cagcacctga
     3241 cggtggaggt gagtgcagct gacggccagt atctggcgca ggctaaatgg gacacgccgc
     3301 gggtggtgaa gggcgtgcgc ttcagtctgc gcctgaccag tggtaaggga acggatgcca
     3361 gactggtgac caccgccatc accgcagaca cggagcaccg tttcagcggc ctgccgctcg
     3421 gggaatacac cctgacggtg cgggcgataa acagctatgg ccagcagggt gaacctgcca
     3481 ccaccacctt ccggattgcc gcaccggcag caccgtcgcg gattgagctg acgccgggct
     3541 attttcagat aaccgccacg ccgcatcttg ccgtttatga cccgacggta cagtttgagt
     3601 tctggttctc ggaaaagcgg attgcggata tcaggcaggt tgaaaccgca gcccgctatc
     3661 ttggctcggc gctgtactgg atagctgcca gtatcaatat caaaccgggc catgattatt
     3721 atttttatat ccgcagtgtg aatactgttg gcaaatcggc attcgtggag gctgtcggtc
     3781 gggcgagcga tgatgcggaa ggttacctgg attttttgta agcgtcgtct cagcaccgtc
     3841 tggcagatcc tgaaattcct gagagaatag tggacaccaa atatggtgga cgctatccat
     3901 gaaatc
//
LOCUS       DAZFDP010000282         3894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFDP010000282 DAZFDP010000000
VERSION     DAZFDP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..643)
                     /locus_tag="ACHJ1G_005120"
     CDS             complement(<1..643)
                     /locus_tag="ACHJ1G_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4766794.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHJ1G_005121"
     CDS             complement(643..972)
                     /locus_tag="ACHJ1G_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766795.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(969..3476)
                     /locus_tag="ACHJ1G_005122"
     CDS             complement(969..3476)
                     /locus_tag="ACHJ1G_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766796.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKVSAGDRQE
                     DSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEK
                     SLLAHKDETLEYKRQLAALGDKVTYQECLNALAQQADKFAQQQRAKRAAIDAKSRGLT
                     DRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEE
                     SATDSMSQVKSAATQTFDGITQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGI
                     VGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVF
                     TKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNG
                     QIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(3457..>3894)
                     /locus_tag="ACHJ1G_005123"
     CDS             complement(3457..>3894)
                     /locus_tag="ACHJ1G_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4766797.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLD
                     MHFSGLTYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVR
                     FGGDGGRDILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
BASE COUNT          774 a         1294 c          956 g          870 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcgatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtcactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca
      361 gatcttccgc catcccggtg accataccgt acaggttaga aaccgtcagc gtggggcgcg
      421 tactggtgcc tttgccattc agttcaaaac cgctcccctg aatgggatac ggctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttcaccacc gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttt
      661 aaactctgcg ctgaactcaa cacgcaacat actgacctgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgcctgtaac cataaggcgg cgtccacaga aaggccttcc agcccccgtg
      781 ctcagccaga aacgactcca gcgccgtggc ctcctcacgg gagacagaca gcgtcacgct
      841 gtacgttttc aggtcagcgt tcagcccggc aggcacacgc tgggaatagc catcaccaaa
      901 gcgcacctcc ctgacggaag gggccgatgt cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtctgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag
     1201 ccgcgcatca gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaactcac cacggtgaac aatccccgct ggctcatatt tgccgccggt tcccgtaaat
     1321 cctccggttg caaaatggaa tttcgccgca gcggcctgaa tggctgtacc gcctgacgcg
     1381 gatgcgccgc caccaacagc cccgccaatg gcgctgccga tactcccgac aatccccacc
     1441 attgcctgct taagcagaat ttctgtcatc atggacagca cggaacgggt gaagctgcgc
     1501 cagttctgct cactgccggt cagcatcgcc gccatattct gtgtaatacc atcaaaggtc
     1561 tgcgtggctg cactttttac ctgcgacata ctgtccgtgg cgctctcttc ccactcactc
     1621 cagccggact tcaggcctgc catccagctc ccgcgaagct ggtcttcagc cgcccaggtc
     1681 tttttctgct ctgacatgac gttattcagc gccagcggat tatcgccata ctgttccttc
     1741 aggcgctgtt ccgtggcttc ccgttctgcc tgccggtcag tcagcccccg gcttttcgca
     1801 tcaatggcgg cccgttttgc ccgttgctgc tgtgcgaatt tatccgcctg ctgcgccagc
     1861 gcgttcaggc actcctgata cgtaaccttg tcgccaagtg cagccagctg gcgtttgtac
     1921 tccagcgtct catctttatg cgccagcagg gatttctcct gtgcagacag ctggcgacgt
     1981 tgcgccgcct cctccagtac cgcgaactga ctctccgcct tccacaaatc ccggcgctgc
     2041 tggctgattt tctcatttgc tccggcatgc ttctccagcg tccggagttc tgcctgaagc
     2101 gtcagcaggg cagcatgagc actgtcttcc tgacgatcgc ccgcagacac cttcacgccg
     2161 gactgtttcg gctttttcag cgtcgcttca taatcctttt tcgccgccgc catcagcgtg
     2221 ttgtaatccg cctgcaggat tttcccgtct ttcagtgcct tgttcagttc ttcctgacgg
     2281 gcggtatatt tcagccgtga cgcttcggta tcgctctgct gctgcgcatt tttgtcctgt
     2341 tgagtctgct gctcagcctt ctttcgggcg gcttcaagcg caagacgggc cttttcacga
     2401 tcatcccagt aacgcgcccg cgcttcatcg ttaacaaaat aatcatcctt gcgcagattc
     2461 cagatgtcgt ctgctttctt aaacgcagcc tctgccttaa tcagcatctc ctgcgcggta
     2521 tcaggacgac caatatccag caccgcatcc cacatggatt tgaatgcccg tgcagtcctg
     2581 tctgcccagg tctccagcgt gcccatgttc tctttcaggc ggcgggtctg ctcatcaaag
     2641 cctttcgtgg cgatatcgtt cgccgcctgt aaggccccgg cctcatcacc ggaacgctgc
     2701 agctgcgcaa catacgcaat ctgctctgcc gtcacgttac ggaactggcg cgccatcgca
     2761 atcagccccg acgtagggtc ggtggtcagt tttccgaaag cctctgcaac cttgtccacc
     2821 tccacaccgg atgcagaagc aaaacgcgcg acactctggt tgatggcatc aaactgttca
     2881 ccaccacgca caccggcatt caccagggct gccagtgact cactcgcctg gttaaacgtc
     2941 agccctgcgg cctgtccggc tctggagagc gtcaacatgc gatcggcagt cagtccggac
     3001 tgattaccgg aaagaaccag ggttttatta aacgctgaaa gcgtggaatc cccctggtac
     3061 caggcgtacg ccagcgcacc tgtcgccacc gccagcgagg tgaccccgac catcggcagg
     3121 gtgatcgcac cggcaagccc cctgaacatg gggatcatcc cgccgaagga gtccttcacc
     3181 tgaccgccct gttgcagcag gatcagccag ggattctgac caccggcaag ctgcgtggcg
     3241 atatccgtaa actgtgcggg cagggttcgc atggccgctt tatactgccc gacggaaatc
     3301 ccggcttttt gtgcagccag cgcctggcgg ctcaggccct gttcaacagc actggcggtt
     3361 tttctggcgt cggtatccag acctgaaaaa tgacgcctta cccggctcat ctgctcatcg
     3421 aaacggaccg catccagact aaggtcaata acaagatcac caaccggctg ggacatatct
     3481 cacacctccg gaaatccccg ctgaagccat cattaatgcg gcatcatcca ccatgacatc
     3541 cgccacatcc gcagacgata aaatatcgcg ccctccgtcc ccaccgaacc ggacgcctcc
     3601 ggcaagtcct gccgctttct gcatcagcat tttgtcctca tccggcctct ccacctgctc
     3661 ttcctcatgc cgggggacaa gcagactgaa atcagaggga tgcatatccg gatcgcaaaa
     3721 aaacaggctg agtacagcgt acgtcagccc ggaaaaatgc atatccagct gggtatcctg
     3781 aaaataatgc gtgcggtaaa aacggtgcca gtcggcatat tcggtggatg tcatcccggc
     3841 aagcatggcg cgccagtcgg gtctccccat ctcacgcgcc agtctgaggg caaa
//
LOCUS       DAZFDP010000283         3829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFDP010000283 DAZFDP010000000
VERSION     DAZFDP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..494
                     /locus_tag="ACHJ1G_005124"
     CDS             273..494
                     /locus_tag="ACHJ1G_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4766798.1"
                     /translation="MVNANPCTRPEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGVL"
     gene            491..1534
                     /locus_tag="ACHJ1G_005125"
     CDS             491..1534
                     /locus_tag="ACHJ1G_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4766799.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRVTGKNLNVEKPAPVTSATP
                     TPAVNVIPNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIEKILDELEGEFYTKSAK
                     SHGTRAGRSFEVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPAPSIPQTLPE
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAIG"
     gene            1699..2499
                     /locus_tag="ACHJ1G_005126"
     CDS             1699..2499
                     /locus_tag="ACHJ1G_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4766800.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPKSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            2529..3374
                     /locus_tag="ACHJ1G_005127"
     CDS             2529..3374
                     /locus_tag="ACHJ1G_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4766801.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALTESKLKISSAATKVKAVLAKTLALFDFLTTPPYQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAKADNLRLSKKRTQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            3543..>3829
                     /locus_tag="ACHJ1G_005128"
     CDS             3543..>3829
                     /locus_tag="ACHJ1G_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO4766802.1"
                     /translation="MLNRRTFNVFCDESCHLLNDHNKVMVLGALWCPGTITKKIARDI
                     KELKLKHNLKPDFEIKWTKVSASKVEFYLDVVDYFFSNPALRFRGVVVPDK"
BASE COUNT         1117 a          922 c          875 g          915 t
ORIGIN      
        1 accttttaat cgttcaggct tatagttccg ccgtcgtagc aaattctgcg accgggtttg
       61 acagcctgaa tgttagtgcg gacaaccgca gatttccgat attgcggtat ttttgtgtcc
      121 gtaaaccgcg ttacgcccga attatggtgg ggcgtgatgg ggaggcttcg gcctgctggt
      181 ttcactaacg ccagtctgtc aaccctgtca cgtcctgcca cctgtttgac agcgggtagc
      241 aggttgttaa acctgttagt gaggccgtaa ctatggttaa tgccaatcct tgcacacgcc
      301 cagaattcat ctggcgtttc tattcctgta aaaaacacca ctatcacttc gttatcgcag
      361 caactgaaga cgaagcacgc tctcaattgc ctgatggccc ctgcattttt actgcccgtt
      421 tttcaactaa ctcgcgcaat tcacttagtt actggaacct ccccttctct gccgacgttc
      481 agggggtttt atgaaaaaac ctctcgtcac ccgtaatgac atagccgaag caatcgccct
      541 gcatactgcc tgtatgccga cacgggagat ccccggcgca attgccaact atttcatgat
      601 aaccaggcgt ttttataccc gaacagataa ggcagtgatc aacaggctac tgatagccga
      661 gatcagggat tatttgattg aacaaggacg tctacgttac gcaacggtgg cagcagaaat
      721 gagaaaggag gcacatagag tgaccggtaa aaatttgaat gttgagaaac cagcacctgt
      781 tacgtcagct acgccaacac cagccgtgaa tgtcatcccc aacaccggag acacaatcga
      841 cagccaaaca ttgttgaaga tggtcaatga ggcacgcaag ttatgtggag aaccagaggt
      901 tagaaacaat aaattcatcg aaaaaatact cgatgaactt gaaggtgaat tttacacaaa
      961 aagtgcaaaa tcccatggca caagggctgg gcgctctttt gaggtcatca ccatgaccta
     1021 caaacaagcc ttgcgagtcg ccgcgcgcga gtcaaaagcg gtccgccgtt cgctgatcga
     1081 caaactggaa gaattgcagc aggcaaactc ccctgcccca tcgatccccc aaacattacc
     1141 agaagctcta cgcctggctg ccgagttggc agaacagaaa atgcagttgg aacagcagct
     1201 ggtggccgca gcccctaaag tcgattttgc cgaccgggta tcagtggcca atggaatcct
     1261 gatcgggaac tttgcaaagg tcgttggact taagcaaaac gcccttttct catggttgcg
     1321 ccagaacggc attctcatgg cttttggtgc gcgcaaaaac gtaccgcgcc aacagtacat
     1381 caacgccggg tatttcacag tgaaagaagt ggtgctggat gatgaaaatg gctaccagat
     1441 acggctgacg ccccaattaa cgggtaaagg ccagcagtgg ttaactcgca agctacttga
     1501 tgctggtttg ttaaaaccag tagcaatagg ttaacaaaag aaaaaaacct gccagcaaac
     1561 tggcaggttt ctgagcagat cgaccaaccc gatctggatc gagttagaaa aatttgctct
     1621 aataaatttc gttttctaag tgcaaaaaat caccatttcg agctggtgat tgaaggttga
     1681 tgcaaatttg gagaaaaaat gcaacaaaca ttcaatgcgg atatgaatat atcaaacctt
     1741 catcaaaatg tcgatccttc aaccactctg cctgttattt gtggtgttga aattacgacc
     1801 gaccgcgctg gccgttacaa ccttaatgct ctacacagag cgagcggact cggtgcccat
     1861 aaagcgccag ctcaatggct aagaacgctg tcagccaaac agctcatcga agagcttgaa
     1921 aaagaaacta tgcagaattg catagtttcg ttcgaaggcc gtggcggcgg cacttttgcc
     1981 catgaattgc tcgcagtgga gtacgcaggc tggatttctc ccgcgtttcg gctgaaggta
     2041 aaccagacat ttatcgacta tcgagccgga agattacaac ctgctattcc gaagagcctc
     2101 ccagaagctc tccgtttggc tgctgacctg gcagagcaaa agcaacggct ggagcaaaaa
     2161 atgctgatgg atgcacctaa agtcgaattc gccgaacgcg ttgctaccgc cagcggggtt
     2221 ctaatcggca actatgccaa agtgctcggc ctgggccaaa actatctctt cacctggttg
     2281 cgtgataacg gaattctgat cgcaaccggt gaacgcagga acgtccccaa acaagaatac
     2341 atatcccgtg ggtatttcac ccttaaagaa accgtgatcg atacaagcaa tggaagcagg
     2401 atttctttca cgactcgtat aaccggcaaa ggtcagcagt ggctgatgaa gcgattgctt
     2461 gatgctggtg tgctggtacc tgtcgcggca acgcgctaac agacgtagta agaaccacca
     2521 gcattgtaat gctggctaaa gtcactttcc tgagctgtat aacgatgagc gattttactt
     2581 tttctggcta tgaattggcc tgctttgtaa cacactccgg tctatcccgt agcgccgggc
     2641 atatcctgtc gcaatgtgca aatctcgcgg caacaaccag tgaatacttc attcacaagc
     2701 ctcaccgcct gatcgcggca gaaactggtt atagccaatc aaccgtcgtt cgtgcattcc
     2761 gtgaagctgt aaacaaagga attctgtctg tagagattgt tatcggcgat caccgtgaac
     2821 gtcgcgctaa cctgtaccgg tttacaccat cctttttggc cttcgcacaa caagctaaaa
     2881 acgcgctgac tgaaagcaaa ttaaagatct cttcagccgc aaccaaggtt aaagctgttc
     2941 tcgctaagac attggcttta tttgattttt taaccacacc cccatatcaa aatgataccc
     3001 cctccccctg tcaggatgac gtggcaataa agaataagaa gtcacaagtt aaaaaaacaa
     3061 aaagatcagt ttccggcggt gccggaacga ccagactcaa aaaattgact tcatggatcg
     3121 ctgaggcaaa agcaaaggct gacaatctgc ggttatccaa aaaacgcact caaaaacatg
     3181 agttcaagca gaaagtagag gcggctgcgc ggaaatatgc ttacctgaag aacaagcgtt
     3241 cgcctgatat tggcgggata tcaaacttcg ataacctacc gcattgcatg acggtaaacg
     3301 aagctcttaa tgcggtttta gccaaaaata aagataacga acaatggggt ataccggcgg
     3361 gattcagagg gtaatgaatt gttctaatta taaccatgca tactttcaac gcctctagtt
     3421 tgccatgagg caaactcata ggcgtcctgg taagagggca ctgttgccaa aactggacgt
     3481 cccattattg caattaataa acaactaacg gacaattcta cctaacaata agtggagtta
     3541 acatgttgaa ccgaagaaca tttaatgtat tctgcgatga atcctgtcac ctactaaatg
     3601 atcataataa agtcatggta ttgggtgcgc tttggtgccc tggcactatc acaaaaaaaa
     3661 ttgctagaga catcaaggaa ttaaaattaa aacacaatct aaaacctgat ttcgaaatca
     3721 agtggactaa agtatctgcg tccaaggttg aattttactt agatgtcgta gattacttct
     3781 tcagcaaccc agcattgcgc tttagaggtg ttgttgttcc tgataaaga
//
LOCUS       DAZFDP010000284         3720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFDP010000284 DAZFDP010000000
VERSION     DAZFDP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..1584)
                     /gene="cadC"
                     /locus_tag="ACHJ1G_005129"
     CDS             complement(46..1584)
                     /gene="cadC"
                     /locus_tag="ACHJ1G_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4766803.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1844..1958)
                     /locus_tag="ACHJ1G_005130"
                     /pseudo
     CDS             complement(1844..1958)
                     /locus_tag="ACHJ1G_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2333..2482)
                     /locus_tag="ACHJ1G_005131"
     CDS             complement(2333..2482)
                     /locus_tag="ACHJ1G_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4766804.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(2482..2972)
                     /locus_tag="ACHJ1G_005132"
                     /pseudo
     CDS             complement(2482..2972)
                     /locus_tag="ACHJ1G_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(3064..3639)
                     /locus_tag="ACHJ1G_005133"
                     /pseudo
     CDS             complement(3064..3639)
                     /locus_tag="ACHJ1G_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT         1016 a          780 c          800 g         1124 t
ORIGIN      
        1 actcaacaac aaatattccc gagcataaat caacccggga gttacttatt ctgaagcgag
       61 aaatttgtcg agataaggta caacataagg aacagaagtc tggaatatac cattttcaat
      121 ccagtaaagg gtgtttgccc ctgggcgtaa attaaaggcg gtgagatatg catcagccgc
      181 ttcccggttc atccccttca tttcataaac cttgccaagc aacacataat ttagccagga
      241 catttcaaga tcaatgccag tatttatcgc ctggtaagac tcatctgttt taccttttac
      301 cagagcactg accgctttta tttgatatat aatggacagg ttattcaatt ccggcagtgt
      361 aacaatgttg tctatttctg tgttcagtgc tgctaattgt ttttcatcta aaggatgttg
      421 agaatggcgc acgatatcaa ctaatgcttt ttctgctctc gcgtaggtaa attctgggga
      481 tgattgaaca atctcaccta ataattcact ggcacgattc aatgatttat catcgccatg
      541 cagtaaataa tcatgtgcct gataaaaatt agttaataac gcaccacgat gaggcaatat
      601 ttgctggagc gtctcctgca ttcgttgtgg ccacggttgg tttaacgctt ttgataaact
      661 ctccagtaaa tcattttgaa tcgccagctg attaccgtta gtgatgacat aacgtttatc
      721 cagcatggtt gaaccatctg cattgtctac caattttatc gacataaagc attgttgagc
      781 acggtattgg cgctgattaa caaacgcaat agataatgtt ttaccggaac tgctcggttc
      841 atcaatgttg tagttgattt tgtcatgcac cataaaggtg gagaaggtgt taagtgatgt
      901 cgccaccaaa tcacccacgc ctatcgcgta agagagctga tacggggaac tccagctgtt
      961 acaactttta tttaccatat taatgtcaat atcgcgtgga ttgagcaaaa tacgcgattt
     1021 gctcatagga agacgtgtat caagagttga aaacgctacc agtgctacac agatacctaa
     1081 cgacaacagg aaaaaaaacc atacccaaaa ggtagtgaat cgtttgcttt taactgggga
     1141 ttgttcaggt ggcgttgcgg tgttttgaat gttaagactg tgggagggag aatctgtggc
     1201 aggaaccgcc tctggtatag ggggaggcga agatagcatt atttcctctc cctcttcttc
     1261 gctgtaccag ataaccggca ccattaattt atagccgcgc tttggtacag tagcgatata
     1321 gacaggacta tcttcatcat tatcttttaa tgacttacgt agttctgaga tactctgcgt
     1381 cacaacatga ttggtgacaa tacttctctt ccagacatta tcgataagtt catccctgct
     1441 aagtacttcg ccactgtgtt gagcaaagaa aaccagaaga tcgattaatc tcggctcaag
     1501 ggtaagttga cgcccattgc ggctaatttg gtttatggac ggagtaacaa gccattcgcc
     1561 aacgcgaact acaggttgtt gcataataga aactcattcg aaaagggaat gatgcaatga
     1621 taattgccac aacctatttt taccatctat agatgggttt atttacatat tattggtgaa
     1681 tgcaagacgt tatttttacc agccataaac ttcttgatta catagtatta cgaaaggatt
     1741 ttactgagaa ccagaagtaa tattccttac catcaaaatt catcatcttt gccaaagaaa
     1801 aatgttcaga aaataatcca tggaaaattg tccggagcgc ttactatttt aatggattgt
     1861 tagtctttgc atgagcaagc ggactgatac atttctcttt gttctcattc agaaaatctc
     1921 atcagtcggc gttcagaccg gtgttgtgct ttatggatgc gttacgctcc tgatgacgtc
     1981 atttgacgtt caacaacatc acggggccgc acgacatttc acgtcagtta gtgctatcgc
     2041 tcaggaacaa attttcccga attgggatat gcccgcaaat tgctggtgat gtggggagaa
     2101 tctggttgag ttcgggagaa ttgatttgga ggcagaacgc ttaaattgtg gcgtccagaa
     2161 acgaaaaacg gacctccgtg gaggtccgtt gatatgaatt tggtgccgga ctcggaatcg
     2221 aaaatttcct ttatcatatt gatattaatg caatttgttt ggtttgatta aaaatgcacc
     2281 ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcacactcgg gatcaggctg
     2341 agagcacaga aggaaagtac ctggcttccc tggcctgttc aatacagggc agcagtgctg
     2401 ccgaatccgt tttatcctca tcgttgtcaa agataattcc cgattcgcag tcgatattgt
     2461 cctgcagcca cgcaatgagg atatacggag aagcacatcc gtatcatcag tgtgacgact
     2521 gcggcggcca tccatccagt catcggttcg tctgagaatg acgtacaact gcgcacgcga
     2581 cacccggaga caacggctga ctaagcttac tccccatccc cgggcaataa gggcgcgtgc
     2641 gctatccact tttttgcccg tccatattca acggcttctt tgaggagttc attttccatc
     2701 gttttcttgc cgagcaggcg ctggagttct ttaatctgct tcatggcggc agcaagttca
     2761 gaggcaggaa caacctgttc tccggcggcg acagcagtaa gacttccttc ctggtattgc
     2821 ttacgccaga gaaataactg gctggctgct acaccatgtt gccgggcaac gagggagacc
     2881 gtcatccccg gttcaaagct ctgctgaaca attgcgatct tttcctgtgt ggtacgccgt
     2941 ctgcgtttct caggccctaa gacatcaatc atctgttctc caatgactag tctaaaaact
     3001 agtattaaga ctatcactta tttaagtgat actggttgtc tggagattca gggggccagt
     3061 ctagtttttt gttatgttgg ctgagtgaca ggatgttaat ctgatgtctt ctacttcatg
     3121 aagattatgt tctaccagac gattgacgat atcagttggt gataaaaatt catctccaca
     3181 cttaagaaga ggtaggccag cagaaccatc ccctggcaga taaattttat tgtatttttc
     3241 tggtgttaaa tcctttggta ggatagtttt catctctgtt gccgcaatac ttatagctgc
     3301 tgcggcagca acatcggtgt tacttgattg tagataactt ctgtttgcac caggaaaggt
     3361 aaactctgtt ttctttttat caaaatcttg ttttgtagct tcgtgcagga ataatatatc
     3421 ataaggtttt ttccctgttg tgactcttga aacattttga ccaagaatat taagagcata
     3481 ttcacctacg gattttgttc ctaaaaacac aagcaatcct tctttctctg gcactgtctc
     3541 tatttttctc cctccaggaa ttgtggagat ggattgagat tgttctgccg gaattgatac
     3601 gctggcgctt ataggtagct gagatcttat actaaacata caacctcctt cttagtttct
     3661 ttaaatgtga gttcatgata attctaatgg agtgagtaat attttagttg ataatagctg
//
LOCUS       DAZFDP010000285         3695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFDP010000285 DAZFDP010000000
VERSION     DAZFDP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHJ1G_005134"
     CDS             complement(<1..173)
                     /locus_tag="ACHJ1G_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766805.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHJ1G_005135"
     CDS             complement(163..342)
                     /locus_tag="ACHJ1G_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766806.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHJ1G_005136"
     CDS             complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHJ1G_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4766807.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(1062..1322)
                     /locus_tag="ACHJ1G_005137"
     CDS             complement(1062..1322)
                     /locus_tag="ACHJ1G_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766808.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            1420..2112
                     /locus_tag="ACHJ1G_005138"
     CDS             1420..2112
                     /locus_tag="ACHJ1G_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4766809.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(2432..2947)
                     /locus_tag="ACHJ1G_005139"
     CDS             complement(2432..2947)
                     /locus_tag="ACHJ1G_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766810.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            3092..3403
                     /locus_tag="ACHJ1G_005140"
                     /pseudo
     CDS             3092..3403
                     /locus_tag="ACHJ1G_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3418..>3695
                     /gene="yfdP"
                     /locus_tag="ACHJ1G_005141"
     CDS             3418..>3695
                     /gene="yfdP"
                     /locus_tag="ACHJ1G_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO4766811.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGR"
BASE COUNT          999 a          901 c          827 g          968 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatag tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgatg cagcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcgcgg aggtagataa ctcgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 tactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttaaaat cacccacagc ccactctgca aagctgtggg ttacaattac cctgccacct
      421 ggtacattaa ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacagt
      541 cacgacacgc cacggcgccc ggagctgcac actcgcgggc gtcattactt tctgaaatgc
      601 aaaaaatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag aggtacagct
      661 cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcaatt gctgaacgaa
      721 tctgttttga ataactgccg atctgttcaa tgacctccag caggcgttgg ttgatatcgg
      781 cgttgtccac atcctcgaca tcaggaagag acacaaagac gccatttgca gactgcgcca
      841 cagcgtcagc aatgaagtga gttccaccag cacgttgcaa aatcattgcc catcccagcg
      901 ggaaaatctg atcgccatcg gcacgaaggc ggttaaataa tgcgttctct gttacatcca
      961 gccagtcagc agcttcagcg tacccccccg gcaacgctgc gatagttttt ctgacagctt
     1021 tcacgtacca ctcaggctgt ttttctactt tccagtgatg cttacccacg gttcacctcc
     1081 tgttcctgtg gtttaaaccc attctggttt tggctagatt gaaaacgtgc cggataaaga
     1141 atctgcattt cgctgacttc acccttaaaa aaattggcta aacgttctgc aagctcgata
     1201 gatggaatct gctccagcct ctcaatacga ctcaacgtcg ctggattgac ttgaacaccc
     1261 gcagcaacat gctgcaaagt gaaaccatgc gccttacgca catttcgtaa tggtgattgc
     1321 atacgtcctc caaatattgc gcgttatgca tgttatttca cgcaattatt ttgcgcaagt
     1381 tgatttgctt atcacgcaat aaagaaatgt aataaacgca tgaacatagg aaaccgagtc
     1441 agacaacttc gccaagcgaa gaacatgaaa atcgccgatc tcgctgaagc gataggagta
     1501 gatgcggcga acatctcgcg cttagaaacg ggtaagcaaa aacaatttac cgaacaaaca
     1561 ctgagtaata ttgccaagag cttaggtgtt gatattgctg atctctttac ctctgcccac
     1621 aaaagtaata ctgtatataa aaacagtaat aatgaggatg ttgcgcaggt gaaggatgtg
     1681 ttccgtattg aaatgctgga tatcagtgcc agtgcgggaa atggccttat ccagggcggt
     1741 gatgtcattg atgtgattca tgccatcgaa tacagaactg ataatgctgt atcaatgttc
     1801 ggcggacgac cagccaatca catcaaagtt atcaacgttc gtggggacag tatgtgtcca
     1861 accattgagc caggagatct catcttcgtt gatgtcagca tcaatcagtt tgatggtgat
     1921 ggtatatatg tctttggttt tgatgacaaa atatacgtta aaagacttca aatgattcct
     1981 gacaaactgc tggtgatttc tgataaccag atttaccgtg aatggggaat tactagcgaa
     2041 aacgaacacc gattcatggt ctttggaaag gtcttaatca gtcagtcgca aacccttaag
     2101 agacataatt aacctcaata tcccatccat cggccaccga aaggtggctt tttattacct
     2161 ataaatttgc atatcacgca aatatcactt gcatatctcg caatttaatt ttatcttttg
     2221 ttccagacca actacaggat tacaacaaaa tctggttgca acacggtgca tgtgtcgtaa
     2281 gcagtcagta aatgtcaaaa acgaacaggc aggacgccca cgaagtagcc gcctggggca
     2341 tatgaagtcc aggatgattc gttagcaaca aaaaagcgcc ctacaggacg cttagctctt
     2401 taacaatctg gatatccaca acagtagtaa tctacagatt gccgttaagt tttctggaca
     2461 actcctcaat ggatggaggc gatacgtaat ccggattttt attcatcaga aacttatttt
     2521 cacagtggag gcacctgctt ttatgaaaaa gctcatcttc gctaaccggg aatggttgaa
     2581 gtatcgatac tatcttttgt ccaaaacatt ttgggcaaag atgcatggtt atgctgccac
     2641 cgttcacgat tacctccttc gagtatacaa aagtacccga ctcaagttgg ttaaggatat
     2701 agccttccgt ctgagcctca aagttttcga attctgcaat tttagctttg agagaagcat
     2761 ttatttcttg ataagagccc accagttcaa cgagagacac gcattcgcgc tgaatagacg
     2821 caagctttga gttcagctca ccaatagccg catttacttc agcttgagtt tttgcctcgt
     2881 tcattagttt tgcaatctgg gctgtttcac gaatagccgt cattgctgcc gttaattcag
     2941 cgatcacatt gaatactctt attgttgttg gggatatcca gattaaccga atccttgttg
     3001 ttggggaata accaggtcca cctcgcctga tgtggctaaa agcaggcaca taacagctaa
     3061 gtattttcaa ccagagagaa tccttagcgt tgtggtgaat gcggctcagc gcacgcgggt
     3121 taaggttgag gctgacagtc gaccttctgt ggatacccac ccgcctggtg tgcaaccttc
     3181 gccaggcacc gggaggcacc cggcaccaca actttatgct gtgtgtagtc ctggcggtac
     3241 cagtttgtac ccttgcttcc ggctggtacc gtcctttttt gcaaaacaga gaagagcatc
     3301 accggacgac gggctcataa cccaatccat ccgggcggct gccaccgcag gtgttcttct
     3361 ctgttttgtg gagaaaccaa ccgaccttgc aaggtcgata tgatgaggag cagcaaaatg
     3421 gctagcgaac gcagtactga tgtgcaggca tttatcgggg agctggacgg cggcgtattt
     3481 gaaaccaaaa tcggcgcagt tctcagtgaa gtcgcttccg gtgtgatgaa cacgaaaacc
     3541 aaagggaagg tctcgctcaa cctggaaatc gaaccgtttg atgagaaccg tgtgaaaatc
     3601 aaacacaaac tctcatatgt tcgcccgact aaccgcggga aaatttccga agaagacacc
     3661 accgaaacgc cgatgtatgt caatcgcggt ggtcg
//
LOCUS       DAZFDP010000286         3662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFDP010000286 DAZFDP010000000
VERSION     DAZFDP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(195..413)
                     /gene="ymgF"
                     /locus_tag="ACHJ1G_005142"
     CDS             complement(195..413)
                     /gene="ymgF"
                     /locus_tag="ACHJ1G_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO4766812.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(545..2068)
                     /locus_tag="ACHJ1G_005143"
     CDS             complement(545..2068)
                     /locus_tag="ACHJ1G_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4766813.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2400..2648)
                     /gene="ymgC"
                     /locus_tag="ACHJ1G_005144"
     CDS             complement(2400..2648)
                     /gene="ymgC"
                     /locus_tag="ACHJ1G_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO4766814.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(2761..3027)
                     /gene="ariR"
                     /locus_tag="ACHJ1G_005145"
                     /gene_synonym="ymgB"
     CDS             complement(2761..3027)
                     /gene="ariR"
                     /locus_tag="ACHJ1G_005145"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO4766815.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(3056..3328)
                     /locus_tag="ACHJ1G_005146"
     CDS             complement(3056..3328)
                     /locus_tag="ACHJ1G_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO4766816.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(3371..3607)
                     /gene="ycgZ"
                     /locus_tag="ACHJ1G_005147"
     CDS             complement(3371..3607)
                     /gene="ycgZ"
                     /locus_tag="ACHJ1G_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO4766817.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT         1124 a          778 c          704 g         1056 t
ORIGIN      
        1 ttgtttaaca aataactctt cgacatcaca atcattgata gttgcaataa aaattatttc
       61 aatgacatat aaaaacaact gtgataacac cagggttgac aactatttgc gcaataattc
      121 ctttacttca atgatccagg cattcactgc cagcaaccca ttcagccgca tactgacagt
      181 gagaaactga aagattattg ctgatccctg tggctgacga gtctttccct taatcgatta
      241 accagattga tcgccagcca aacaagggca ttaagcaaat ttatcgtcac aagcacttct
      301 cccagcccgg tactgggttt tgcaataagg atgaccagga gggtgatcag cataaatgca
      361 atggaaaaga tgatatagag agtgaaataa tccagattat tactattgtt catgctacta
      421 atggcaatcc tgtcaaaact ttcaaacaat tatagcaaac aaaataatga acaatagtaa
      481 caagaggcaa cgagctaatt agctgtctat aattacaccg cactatggcc ttactgatca
      541 attttcactc aaccacaacc ttcaccttcg gtttagaaag gataattttg accaggtcga
      601 tgtatgtaac aggtttatag aaataataac cctgctgaaa tgtgatatta ttttggttca
      661 gatagtcaag ttgttcattc gtttcgacac cttcagcgac gatactgatc gaaagtttac
      721 gcgccagatc caatacacaa tccagaattc gggttgattc tgggtctgcg tttacgcggc
      781 caacgaaact gtgatcaatt ttgatataat caatatgcag gtcatgaaga taagaaagcc
      841 ctgagtagcc agtaccgaaa tcatccagcg cgatgacaaa accattttca tgcagaatgt
      901 tcaaccgctg aaccagactt tcatcaacat tcaatggctc acgctcggtg acttccagaa
      961 caaggtttaa atcgcggcgg gtaaaactgt cacggaaatt taaacactcg tcgacaaacg
     1021 tcggcgaaat aatatgcgaa gcgctaaaat taattccaat atgaaaacct tccggcaatt
     1081 tactcgcgat agcgttcatc tgtctggcaa cctgattcat caggctttgc gtaagcggaa
     1141 cgattaatcc ggatttttca gcaagtggaa taaatgccgc gggtgatata tatccaccgt
     1201 gaggttgttt ccagcgggct aacacctcaa ctccccgcaa tgtcccttcc cgaccattta
     1261 ccacaggttg gtaaaaaggg acgatctccc cattatttat tgcccgtcgc aggatctctt
     1321 caggcgttgc gcttttattg aaatatctat caagcaggaa ggcagcggcg caggcgatta
     1381 acagaataaa tattaaaatc ccaaagcctt tatcgataag tctgtagaag ctaaagagtg
     1441 gcggcgggtt aaagataata gtgaaatgat attttgtgga gtgaaccttc tcggtatagg
     1501 gataatgtcc attaagcgtc atcacatcac ccgttggtcc aataatgtca tccgccacgc
     1561 gtaatacata ccttacccct ttcaagggta cattaagtgc cccacgaata tgctgatcgc
     1621 ttatcgtaac caaaatgcgc gtatctgcaa attggttctg atagagcagg ataggtaatc
     1681 tattaacggt gtcgattgct ggagccaaca gtaaattact gtccgggaaa acaggaatac
     1741 gagacaacag gacccgattc ccaggcaggg atgtacacca gactattccc tgtttgagaa
     1801 tgatgattgt gcgcaggtga ggtttcagag ctgcttcagt gccaagctga tattgctcct
     1861 cgaggttgca ttccttcccg gcaatgttaa ccgctgtttg agtcgcttgt gacgcttctt
     1921 caagtatatg attgatattg ttggcggcat atctcgctcc cgccaggtac tctgcttttg
     1981 cggaatacca gagctggatg ttcaaaatag cgacaccggt aataaacaag catatcgcaa
     2041 cgaggatggg tatgagtgta ttgcgcattt gcttgtcccg gccaaagggt ttaatacatg
     2101 aaaatgccat tcattcgctc gcgctggcat ctacagagag catggtagag agcataagaa
     2161 atatccacgc cgaagataaa attttctcca tcttgatcta acccattaat tattgagcat
     2221 aatgtaggca gtacaaaata agttaggcgg gatatcaggc gtcaagaatg gagagcgaga
     2281 actctccatt cttgacacct gatattgcgg acataataag aaagcataac gcctgaaatg
     2341 ctcactttgc atcagcatgg tgatacagct gatgtttatt ctaaaacctt actcaagttc
     2401 taagagagca cggattccct gtcattaata atgatatccg ttagtgtagt cttatgcatt
     2461 tcaatgtact gcaatatttg cagatagaga tcagaaagaa tcacactggt ttcatctttt
     2521 aaacgcaagc gtaaagcctg atcaataata tgactatcaa catacccatg cgtcatgaac
     2581 acctccctct cgagattttt aaacaaagca cattgaaaaa taaatctctc tgggattgaa
     2641 ttattcatgt cagcctcgta gaaatattaa cccaataaaa ttatgctgtg tatagtctaa
     2701 agcgacagac aaaaataaga tgaatgagtt ttctataaac ttatacttaa taattagaag
     2761 ttacatatca tcagctgtgt atcgcaacac gatttccagt gtttttctaa tcacatcagc
     2821 tttgagaata tcacttgttg tttccaggga gtgtatcaga cttaagataa tatttttatt
     2881 attaacatta tcgcctgaaa gcattaaatt ggtgacagcc tgccctaaca ctgctgactc
     2941 ttcttctaac aaattacccg aactgcgaaa gtaacttgct aacgctttat cagttattgc
     3001 attatgaatt gtagtatctt caagcatggt aacctctcat cttacttatg aaattttaat
     3061 gtattctgtt tattttctta ccattgaaca tacttttatt gtcagctgtc gctgttcttc
     3121 ggtttggccc tcctgaatca ctattgctaa aatcacgaat tatgctgtca attaaatcgc
     3181 gtcggaaagc atcttcctca gccgatttca ttacgagtaa ttgcttaata agagtctgca
     3241 atgaaaaatt tatcttcatg cgcagtatct ggatcaccgc ctggccagta atttcatatt
     3301 ttatacgttc attatcagat gtcttcataa cgacttcata gctaacctgt taaataatca
     3361 ggctgttgca ttattcaaaa agcaacccaa ttagtgcatt ataatgtttc tgttcctctt
     3421 ccccagtcgc gtgttccagt cggcaaagaa gtttggcaca aagcgacttt cgactcagat
     3481 tacgtccatc tttcaaaatc tcagtcacaa tttccccgag agtttcctgc tgagtaggca
     3541 agttagcttt tgcaaagtaa cgagtgattg ctcccgcaga atctaaagtc actgaatttt
     3601 gatgcatgct acgcctctgt taaaaatgtt aatcaatgtt aagtgaggta acaatagcaa
     3661 ac
//
LOCUS       DAZFDP010000287         3642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFDP010000287 DAZFDP010000000
VERSION     DAZFDP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..1249
                     /gene="proB"
                     /locus_tag="ACHJ1G_005148"
     CDS             146..1249
                     /gene="proB"
                     /locus_tag="ACHJ1G_005148"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4766818.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1261..2514
                     /gene="proA"
                     /locus_tag="ACHJ1G_005149"
     CDS             1261..2514
                     /gene="proA"
                     /locus_tag="ACHJ1G_005149"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4766819.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2629..2704
                     /locus_tag="ACHJ1G_005150"
     tRNA            2629..2704
                     /locus_tag="ACHJ1G_005150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2662..2664,aa:Thr,seq:cgt)
     gene            3309..3467
                     /locus_tag="ACHJ1G_005151"
     CDS             3309..3467
                     /locus_tag="ACHJ1G_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766820.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          916 a          870 c          982 g          874 t
ORIGIN      
        1 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
       61 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
      121 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
      181 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg ttgaacttgt
      241 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
      301 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
      361 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
      421 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
      481 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
      541 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
      601 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
      661 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
      721 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
      781 cggcatgagt accaaattgc aggctgctga cgtggcttgc cgtgcgggta tcgacaccat
      841 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
      901 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
      961 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc ttgaacgcgg
     1021 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     1081 catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc gttacaacag
     1141 cgatgcatta cgccgtattg ccgggcacca ctcgcaagaa attgatgcaa tactgggata
     1201 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagcaggctg
     1261 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc
     1321 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     1381 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gtgccaatgg ccttggcgaa
     1441 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgatgatgtg
     1501 cgccaggtgt gtaacctcgc cgatccggtg gggcaggtaa tcgatggcag cgtactggac
     1561 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     1621 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     1681 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     1741 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     1801 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     1861 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     1921 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     1981 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     2041 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     2101 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     2161 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     2221 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     2281 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     2341 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     2401 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     2461 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     2521 ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg cccggttagt
     2581 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     2641 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     2701 accattaaga aaatcaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     2761 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     2821 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     2881 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     2941 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     3001 atagtggatt aaagcccacc tcttcaaaac ctaacttgat cgggaaagaa tcaagctcta
     3061 gcacggtttc gtccaccgga gtacaacggg tgcctgataa gagaagaact gctaagactt
     3121 catagctttt gtgaaaaagc cctttttagt ggatacgcct acggcgctta ttttttggta
     3181 taaccatcaa aggcgcttta tggctcctat tgtcttgaaa atgtatcttt acttgtattg
     3241 tgttttatgc gtagtctccc tgtgtagtat gtaacttgtt aattttcata tgaatacaga
     3301 ggcacacgat gaaaaacatt gcagctatca aacgtaataa ccgcaagatt cacgctcgta
     3361 agttcctgtc tacgccagag ggaaaagcct ggctagagcg aaagcaacga gagaacgaag
     3421 aaagaaaact ccttagtgag ttgaaatggc ttagagagga tttctgaaaa tctccagaac
     3481 gcaacaggac gcattctaac gctgttttca ttgcttggca tagggatgta taggtgatgt
     3541 gtgtttaatg cgtatgtgag cttgttttgt gccttatttt gcattttcat aatgcagaag
     3601 agaacacgga aaaccttaaa attaattatt ggggtattta ag
//
LOCUS       DAZFDP010000288         3625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFDP010000288 DAZFDP010000000
VERSION     DAZFDP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1020
                     /locus_tag="ACHJ1G_005152"
     CDS             <1..1020
                     /locus_tag="ACHJ1G_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4766821.1"
                     /translation="NVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGFNGHVGT
                     TFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQEEKGYHE
                     NETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNSFRPTLT
                     YFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIEFFYQKK
                     HNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSLQYNANR
                     TYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQDNIYVH
                     AFYRF"
     gene            1039..1995
                     /locus_tag="ACHJ1G_005153"
     CDS             1039..1995
                     /locus_tag="ACHJ1G_005153"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4766822.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            2086..2574
                     /locus_tag="ACHJ1G_005154"
     CDS             2086..2574
                     /locus_tag="ACHJ1G_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO4766823.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            2657..>3625
                     /locus_tag="ACHJ1G_005155"
     CDS             2657..>3625
                     /locus_tag="ACHJ1G_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4766824.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKY"
BASE COUNT         1171 a          653 c          760 g         1041 t
ORIGIN      
        1 aatgtcttat cggcttcaat tgcaacagtc cttttaggct caacaggagc ctttgcggca
       61 aacgatcgcg gctttacctc cgacactgac tggtcagaag aacaagcggg ctttaatgga
      121 catgttggta ctacatttga atatgagacc aaaagcactg ataactattt aggtaaaaca
      181 gtaaaggaat atactaaaac acacgagatt ttccaggcct atttcagcca acctaattgg
      241 caatttgcag caatctatgg ccttaagtct atcgatcgcc ggcaagaaga aaaaggctat
      301 cacgaaaatg aaacaagtct gcaaaactat atatcactaa acaaaacgtt cactattggt
      361 aacggtttcg acttcagtct ggtatacgac ctgatgtata ccgacggaaa tatagatagc
      421 ccttatgtta caggcttgca tactgtaact gttgacaaca gttttcgccc tacgttgacg
      481 tactttaact ctgactggaa cgcaggtttt tattcaaacg ttgaatactt atacagtcga
      541 aatgataaga gctcctgggg cgtacgcgaa gaagaaggtc aaagtatact atttcagccc
      601 tataaacgtt ttggtgcatg ggagtttggt attgaatttt tctaccagaa aaagcataac
      661 gatgaattta atcacggtaa aataaatgat cgtagtattt ataccgaaac ttactacgag
      721 ccaattatca aatatgcttt tgaaaacgca ggtaatctgt atctacgcac ccgcttcgga
      781 cacgggaaaa cagaaaacgc agatagtttg caatacaatg caaacagaac ctatttcaaa
      841 acaattcgaa aagccactct aggttatgaa caaaaaatag gtgacgactg gatagccaag
      901 attgaatata aacatgcctg ggaaaaagag cacaaaaact tttatgatcc aagagatgaa
      961 gaaaaatata atgtgctcaa tcaggataat atttatgttc atgctttcta tcgcttttga
     1021 ttaaacagag ggaaacctat gttcagcgca ctagatatta aaacgttgat gcaaggaact
     1081 ttatatggtg atccatcact cagggttgat accattcggc caatccatag tcctttagtt
     1141 ggtggactct cgattgttat gacacctgga gatttgttgc atataccaac gactggtgcg
     1201 gatataatta tcggaccgga agagatcatt agttctaatg ctaaggcaaa gattgtagtc
     1261 gattacctga acgtgaataa tcttaacaaa gttttaagat attttaaggt acataagtat
     1321 cgtttgtttg agcaagaaaa cacttcaacg atacctgatg tatacattgg taaacactgc
     1381 cagataggta tgaactgcca ttttatgcct ggtgttaaaa ttatgaactg tgtgaccata
     1441 ggtgataatg ttgccattca tgcgaataca gtgattaaag aaggtactat tattggcaat
     1501 gacgttatca tagattctaa taactccatc ggtaattata gttttgaata tatgtcggat
     1561 gaacgtgatt catatgtccg agttgatagc atcggtagag taatcatagg tgatgatgtt
     1621 gaaatcggtt gtaacaacac cattgaccga gggacacttg gagatacgat tataggccaa
     1681 ggcactcgta ttgataatca agtgcagatt ggacatgact gcataatcgg taataaatgc
     1741 ctgattgttt cacaatgcgg tttttcagga cacgtagttt taggcgacca tgttattaca
     1801 catggtcaag tcggaatcgc cggtcacata tcaattggta gttattcggt aattaaggct
     1861 aaatcaggtg taagccattc ttgtcctgaa aagagtgacc tttttggtta ccctgccaaa
     1921 aatacgcgag aatacaacaa aaatctcgct gtccttaata aactaactaa acagcatggt
     1981 gtatataaac agtgagtcgt tgagaagtat tatttatata cattaacatt aacactgaca
     2041 tagaaatagc ttaactaagc tatactactt ttattgagag tttaaatgaa aaataaattg
     2101 cttattgcgg ctgtgttgac tggtcttctt tcaggatgcg ttgatggcaa ttcaccctct
     2161 ggcagtcagg aaggtgtcgt gaaagcggat cctcgctatt cagaatgtaa cctccctgat
     2221 gtaaatgaaa gagggccaat tgctgcgggt ctttacattt ccggaaccct cccggaaggc
     2281 tattggttaa tgaagccaga gcgtaaaatg cattataagg gagatggtat ttatcagctt
     2341 atttattctg aaaaagcagg tgcggtaaca atgcaatttg cgactatgaa ctggaacccg
     2401 cagttcactg taacaggtct tgaattgcca ttaaatcagg ctaaaacact caagaaagga
     2461 ggcatggata aaaatacaaa agtcgttatt cctgccgatg gaaagtatgt ttggacgatt
     2521 caaattggtc ctgataaaaa ggcaatagcc gccatggtag caccatgtaa atagttattt
     2581 ttagcactcc actattcaac attcgttgaa tagtggagta tcagtattcc aaaattcttg
     2641 ttaatgagag cagattatgg ccgcggtaat tctacgcaaa gttgaaaaaa tttatgacaa
     2701 tggattcaaa gctgttcacg gcattgatct cgatataaac gatggtgaat tcatggtttt
     2761 tgttggacca tctgggtgtg ccaaatcaac tacattacgc atgattgcag gtcttgaaga
     2821 cgtatcaggt ggtgaaattt acattagcga ccgccttgtt aatacccttc cgcctaaaga
     2881 tcgcagcatt gcaatggtgt tccagaacta tgcgctgtat ccccataaga ctgtatttga
     2941 caatatggct tttggtttaa aaatgcaaaa aaggccaaaa gaagagatta agaaaattgt
     3001 cgaagaagtt gcagaaaaac ttgaaattag cgatttactt taccgaaaac cgaaagaaat
     3061 gtcgggtggt cagcgtcagc gtgtcgcggt aggccgtgca attgtccgta agccagatgt
     3121 tttcttattt gatgagccac tatctaacct tgatgcaaaa cttcgtgttt caatgcgagt
     3181 caaaattgct cagttgcatc agtctctcaa ggatgagggt catccagcaa ccatgattta
     3241 tgttacacac gatcaaattg aagcattgac cctcggtgat cgtatttgtg tgcttaatca
     3301 ggggcgaata atgcaggtcg acacaccagt taatctgtat aaccaccctg ctaacagatt
     3361 cgttgctggc tttattggtt ccccgtcaat gaatttgata gatgcggttg tacgtgagga
     3421 agacggtaaa ctctatattg acattgccaa aaatattcgg ttgtacatac ctgagaataa
     3481 acaatcagta ctcaaagcgt atatagataa acccgtttgt tttggtattc gtccagaaca
     3541 tatctctttg agtttgaatt gcaaagaaat gaatacagtc actggtgcat taagtattat
     3601 tgaaaatatg ggcagtgaaa aatat
//
LOCUS       DAZFDP010000289         3555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFDP010000289 DAZFDP010000000
VERSION     DAZFDP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(263..646)
                     /gene="traM"
                     /locus_tag="ACHJ1G_005156"
     CDS             complement(263..646)
                     /gene="traM"
                     /locus_tag="ACHJ1G_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO4766825.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            969..1571
                     /locus_tag="ACHJ1G_005157"
     CDS             969..1571
                     /locus_tag="ACHJ1G_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO4766826.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQNN"
     gene            complement(1868..2689)
                     /locus_tag="ACHJ1G_005158"
     CDS             complement(1868..2689)
                     /locus_tag="ACHJ1G_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4766827.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEKFREWKS"
     gene            complement(2799..3095)
                     /locus_tag="ACHJ1G_005159"
     CDS             complement(2799..3095)
                     /locus_tag="ACHJ1G_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766828.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            3395..>3555
                     /locus_tag="ACHJ1G_005160"
     CDS             3395..>3555
                     /locus_tag="ACHJ1G_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766829.1"
                     /translation="MAPDRTTGRDPPQRREARFAKRRCALHPGGLLPGCQERRPRRDR
                     GGATGTVAA"
BASE COUNT          999 a          817 c          750 g          989 t
ORIGIN      
        1 gacaaaaaac tgtttatcca aatatatttg acgttcagta ttaagttgtc taggatccgt
       61 cggatacata ggaacctcct ctcaaaggat tctatggaca gtcgatgcgg ggaggtcgct
      121 ctccctgcat cggcgatttt taatatctgt tttaacgtgg cattaaccac ataattaata
      181 taacctttat gtggttaatt gtcatcaaat tgaaccagat caaaatcctg ttatttgaag
      241 taaatcaact tttaattctt ttttattcct catcattttt tggaaaaaat cgttccatct
      301 cagatgacac cttctcacga atatcttcaa ccatattggc atattcaaac tttggattcc
      361 cggagacatg gggacttaga gactcaatac ccaaaatttt cgctactgat gactgtgttt
      421 ttacaacaca ttcaagaaga agtttattaa actctgtctg attaaacgca gactctttac
      481 gctccatctg agcctcatat acacgaagcc ccagttcaag aagcattgaa gctgttcccg
      541 aaacacttat atctttatct cttgccccct cctgacgacg ctgttcaact atcgcattta
      601 ctttatcata gacatcatta ctgatataca aaattactct ggccataaga taaaaccttt
      661 cattattaag caatgaactt ttcactataa atatgcatat agtgtttaca agtaagaaag
      721 acactcctag cagcgcctct aggatcatcc tataaaaaaa tgcgatccgg cgctaggggc
      781 gtccctaata tatatcaatg tttttcatga aaattgtcag tactgatcct aataagagtc
      841 gctatagggt cgtaacagga tcgccaacga ctctctattt aataattcag aattattaaa
      901 tataaatagc gtttgttaat tacatgattt aaaacgtaaa tcagcaaaaa cttgtttttg
      961 cgtagtgtgt ggtgcttttg gtggtgagaa ccaccaacct gttgagcctt tttgtggagt
     1021 gggttaaatt atttacggat aaagtcacca gaggtggaaa aatgaaaaaa tggatgttag
     1081 caatctgcct gatgtttata aatgggatct gcgaagccgc cgattgcttt gatcttgcag
     1141 gtcgggatta caaaatagac ccggatttac tgagagcgat atcatggaaa gaatcccgtt
     1201 accgggttaa tgccattggt attaatccgg taacgggata tggcagcgga ctgatgcagg
     1261 tagattccca gcattttaac gaactggccc gctatggaat taagccggaa catctgacaa
     1321 cagatccctg catgaacatt tataccggtg cttattatct ggcaatagcc tttaaaaaat
     1381 ggggagtctc ctgggaggcc gttggtgcat acaatgccgg attcaggaag accgaacgcc
     1441 agaaccagag acgtcttgcc tacgcatcag aggtttaccg gatttatacc tggataaaga
     1501 gcagtaaagg catccgggtt ccgaccacga agaaatcact ttcccaaatt aacagtgtgc
     1561 agaacaatta atattgtata cattgtcaaa taccggttat gccgcgacag cggcatggaa
     1621 ggccacacac aaaaatatca tatgagtgat aatgctgact ggccgaagcc ccgcaaagcg
     1681 ggaccccatc ttatatatat ccggtaactg cgcagcctcc tgccagacca tttttcgcgg
     1741 gcacaatatt tacagactac cgaaaagcca gttgatgaaa ttgaggccta tcagacgaca
     1801 cagcaatgcc gcaggatgat aatataagta aagccccgaa aaatttttcg gggctttact
     1861 tatattatca tgacttccac tccctgaact tttcggcaat catccataat gctttgttga
     1921 ttcggatatc accgtcaatg cctgtaatgg ctcttgttct ggtattttta ccggaggcac
     1981 ttcgccccga taatccacct tttatcatat tctcctgaac ccgctgccag gttgtccaga
     2041 gatcgttctg tttatcctcc caacggcggg gagtaattat ttgttctggc gtcactggcg
     2101 ttttattttc atcttcgtac ctggccatca atgcagctct gccaaataaa tgttgttcgt
     2161 cactgttaag atgtatctct ttcattgcct ccatattttc agtgacttta tcaaagacac
     2221 cgagcacttc atacgctccc tcaataacct gcccgacaat atcaccttta tgtggaacgc
     2281 ggatttcgcc aaaattattc ccacacacta ggccatttgt gcagacaaaa cgaaaaattc
     2341 ctgggatcat ctgataactg gatgaaccat catgtgaatt aagcaggata atttcaggaa
     2401 cttctttccc gttaatatgc ccttccctgc gaagacgtaa catatgttta ctgtattcgc
     2461 ggcgtcctaa atcacgaacc cgactctgac aggcaaagaa cggctggaaa ccttcatcac
     2521 gtaacttatt gatgatattg atggttggga tatatgtata acgctcactc cgggattcat
     2581 gtttatcacc agaaaagaca gatggaacaa actgcattaa ttcgtcatcc gttaacgggc
     2641 gctcacggcg aatagaatta tatcgaccaa aacgggaagc taatcgcata acagtaactc
     2701 ctttatgaag gcataaaatt aataaaagtt ttttcgctta atatcatccc cctcggttat
     2761 cgttaacaaa aagacaacaa ttccgagggg aaggaccatc aaatcaggtc agttcgggac
     2821 tgaccaacct gccgagtgaa aagcagtaac agcgcgatca actcatcagc gttttttctg
     2881 acaagaggag tatgattctc caggctatcc agtaagtcat gcagctcatt attgacctgg
     2941 tggagcacct taaagagtgc accagaatcc cggcagctct gatatgcaag atttgcctga
     3001 acagcacgat tctgcatgat cccgatttgt gcgattgcgt ctttctgctg atcgcctgtt
     3061 ggctgataag tggtgttaaa agaaaatgcg ctcataattc ctcctgtatc tgatgttttc
     3121 agcccccatc atctgttgag tttcgggagc cgcggttttg acgttttgcg gggacctgcc
     3181 ttttgttccc tccggtattc accatcacct gtttgagaac cttcgccgca aggggcatca
     3241 ctgccacggc ctgacgcacc tgtcctgggc agaacgcaaa attttttacc gggttttggg
     3301 taaaaaattt tgtgggctgt tcatttaccc tggacagtca gtgcggcagg ttgtggctgt
     3361 ctatgccccc ggcgacaggg gcttaaacag gtgaatggca ccggacagaa caacgggcag
     3421 ggacccgccg caacgacgtg aagcgcggtt tgcgaaacgg cgttgcgcct tacatcccgg
     3481 cggccttttg ccgggatgcc aggagcgcag gccacggcgc gacagaggcg gcgcgaccgg
     3541 aacggtcgct gcaag
//
LOCUS       DAZFDP010000290         3546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFDP010000290 DAZFDP010000000
VERSION     DAZFDP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            139..780
                     /locus_tag="ACHJ1G_005161"
     CDS             139..780
                     /locus_tag="ACHJ1G_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4766830.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            811..933
                     /locus_tag="ACHJ1G_005162"
                     /pseudo
     CDS             811..933
                     /locus_tag="ACHJ1G_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(1721..2155)
                     /gene="uspF"
                     /locus_tag="ACHJ1G_005163"
     CDS             complement(1721..2155)
                     /gene="uspF"
                     /locus_tag="ACHJ1G_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4766831.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(2296..3429)
                     /gene="ompN"
                     /locus_tag="ACHJ1G_005164"
     CDS             complement(2296..3429)
                     /gene="ompN"
                     /locus_tag="ACHJ1G_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO4766832.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1030 a          678 c          796 g         1042 t
ORIGIN      
        1 atgagaaaag atgaatgtca ctttgataca aaaagagaag ctttttgttg taagtgataa
       61 ttaatgatat aaatatagtt ttatgtgctt ttatttttta ctaaagcaaa attaagttat
      121 taaaatggag ggagaggaat gcctgtagat ttaacgcctt atattttacc tggggttagt
      181 tttttgtctg acattcctca agaaaccttg tctgagatac gtaatcagac tattcgtgga
      241 gaagctcaaa taagactggg tgagttgatg gtgtcaatac gacctatgca ggtaaatgga
      301 tattttatgg gaagtcttaa ccaggatggt ttatcgaatg ataatatcca gattggcctt
      361 caatatatag aacatattga acgtacactt aatcatggta gtttgacaag ccgtgaagtt
      421 acagtactgc gtgaaattga gatgctcgaa aatatggatt tgctttctaa ctaccagtta
      481 gaggagttgt tagataaaat tgaagtatgt gcatttaatg tggagcatgc acaattgcaa
      541 gtgccagaga gcttacgaac atgccctgtt acattatgtg aaccagaaga tggggtattc
      601 atgaggaatt caatgaattc aaatgtttgt atgttgtatg ataaaatggc attaatacat
      661 cttgttaaaa caagggcggc tcatcctttg agcagggaat caatcgcagt ttcaatgatt
      721 gtaggaagag ataattgtgc ttttgaccct gacagaggta acttcgtttt aaaaaattaa
      781 gatagcaatg gcgggttaac ttttcgtaaa atgaaattaa ttctcggtac ggcatgtaaa
      841 acgcggcacc tcgtatgcaa gaacgtactg cggttggttg gtgaactttc gatagtgcta
      901 gtattggatg atttccagcc gttaccgatt ttatataact tcagtggaat tattcaacac
      961 acaggcaaaa cggaataagc tgagaaaaaa agcgtgcttt cccaatctgg ggtaaatcac
     1021 gtctgaatga cacctgaaaa caggtggtgg cctcaagaag gcttggaata ttcttcttta
     1081 aatgttatgt aatttattga tttttcgtgt acgattttaa gacatttatt ccaagaaaaa
     1141 tttttaactc tttgattttt cgactctgta tcatcggtct cgaaacccgt agccacgtcg
     1201 tatgcaagaa cgtgccgcgg ttggctggta agcttttcga tagtgcgagt atctaatgat
     1261 ttccagccgg aactgattct attggaaaga aatcatgggc gcaatttaca gatcacagat
     1321 tgattagtta tagattttta ctgtggcaaa aatgggacaa aaggccgcaa aaggggcaaa
     1381 aatggggcaa aaaaagtgta gattattgtg ctttattgta gttgttaatc ctgcctaaca
     1441 tattgaaaaa cataaaagtt attgttcgtc agatagttgc ggtttttact ctgacttgtt
     1501 caggttgtat tgttctttct tactaatttc ttgattttgc gacatttaaa agcgactcaa
     1561 ttcgttatat ggcatcagaa gagtatgcgt catgccggaa cgcccagcat aagaaatctg
     1621 atataaaaac tgtggcgtgt atggtacgga ttagagggga aaatgtcagc acatttgcga
     1681 aatgaatcaa aaagcccgca gcaatgtgcg ggcgttagtg tcagcgcaca accagcacgg
     1741 agcactctgc gtgacgcact acagctgcgg cgttagaacc gagcagataa gtggtgatat
     1801 ccggtcgatg ggaagcaatg atgatcatat gagcggggat cttcttcgcc aattccagaa
     1861 tgcggtcttt tggcgagcct tcctcaacat ggacatgcac tctgtcggtt ggcagtttaa
     1921 attttttaat gatctcttcc agttgcgatt tggcttccgc tttcaggtca tccattgccg
     1981 gtaattctgc ggaatacgct aaacccagag aggcatagta gggcagtgaa ggtattaccg
     2041 tcaggaaatg aacctctgca tcatcaatct ttgcctctgc ctcaacgtgg ctaatcacgc
     2101 gttgagttaa ttctgaatcg gaaatatcga tagggacaag aatcgttctg ttcataaaac
     2161 ctcctgtttt agtatccgca taaagtgtaa cgccagatga cactttttgt gtaatgacgg
     2221 agttcacatt tttaatttag atcaaaggag gaagaataag cagaaaaagc ccgccataac
     2281 agcgggcagg aggatttaga actgataaac cagacctaaa gcgacaatat catcggtaga
     2341 gatgccgttg gcagcgtaga aactgtcatc ttcatccaac aggttgattt tatagtcaac
     2401 ataagtggac atgtttttat tgaaatagta agtcgcgcca acatcggcgt atttaaccag
     2461 atctttatca tcaacacctg ccgggttgtc tgcaccaccg gcagcgtgca ggtcacggcc
     2521 tttagacatc aggaaagaga ctgccggacg cagaccaaaa tcaaactggt actgtgcagt
     2581 gacttcaaag ttctgggttt tgtttgcaac agcataatcg ctgtcgccaa acggggtcat
     2641 attacgcgtt tctgaataca tggttgccag gtaaatattg ttagcatcgt attttagccc
     2701 agcagtccac gcgtctgctt tatcaccacc cgccgcagta tggttaacct ggtcattggt
     2761 gcggtcagaa gaggtgtatg ccgcaccagc gctaaagccc atgcctaaat catatgttgt
     2821 ggaaagaccc cagccgtcac cgttttcatg gcgaacatca cgtccgttgt tggtgccttc
     2881 ctgaccatta ctggcacctt cgttgttacc ttgatactgc accgcgaagt tcagaccatt
     2941 taccagaccg aagaaatcag tattacgata agtcgcgacg ccattggctc gaccagtcat
     3001 aaagttgtct gcattggtat aagagtcacc gccaaattca ggcagcatat cggtccagcc
     3061 ttcgatgtcg tacattacgc cataattacg tccgtaatcg aaagaaccgt aatctgcaaa
     3121 tttcagtccg gcaaatgcca gacgggtcca tgactggttt tttgaagatt cagtgttgtt
     3181 tgcctgaata ttgtattccc attgaccgta gccagtaagt tgatcgttaa tttgggtttc
     3241 gcctttaaaa cccagacgcg catagctctg gtcgccatct ttcgctgaat tatcagaaaa
     3301 ataatgcagg ccatcaactt tgccatacag atctaatttg ttgccgtctt tattataaac
     3361 ttcggctgca tgtgcagcac ctgcggcgag cagggcagga attaaaagtg ccagtacttt
     3421 gcttttcatt gaataaatcc tttagttatt ttatttgcct tttatcccca ttttggggag
     3481 tgaaaacatc ctaaaggaga agttcagtta gccgataaat atcatttgtt acgccttaag
     3541 taaaac
//
LOCUS       DAZFDP010000291         3508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFDP010000291 DAZFDP010000000
VERSION     DAZFDP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..511)
                     /locus_tag="ACHJ1G_005165"
                     /pseudo
     CDS             complement(1..511)
                     /locus_tag="ACHJ1G_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(745..1422)
                     /locus_tag="ACHJ1G_005166"
     CDS             complement(745..1422)
                     /locus_tag="ACHJ1G_005166"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO4766833.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1620..3239
                     /locus_tag="ACHJ1G_005167"
     CDS             1620..3239
                     /locus_tag="ACHJ1G_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO4766834.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            3236..>3508
                     /locus_tag="ACHJ1G_005168"
     CDS             3236..>3508
                     /locus_tag="ACHJ1G_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766835.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTL"
BASE COUNT          866 a          895 c          854 g          893 t
ORIGIN      
        1 taatatattc tactgatttt tgtagtttaa acgatttatg aagaatttta ctgttgtaca
       61 gtaccagata ctcagccagt ttcagattga acatattcag ctattcaaag agcaattctt
      121 cattaaactc aataaattgt tccctcagtg ttcggttaaa atgctcactt atggcattta
      181 ttttcggcgt atagagataa gtccatacga acccgacggt ggcttcctgt agcgttttgt
      241 cgaattttcc cgagaactct ttgcccatca tcagtgatga tctggttgat accaacaagg
      301 aacaactggg ctacacagca gaagaaatgc ctgatgatat cacttttaag cgatagtacg
      361 gccagagagt agttgctgtg ttcgtcgagc atggtaatga aataacggta cagctcgcca
      421 ttctgagctc aatagtatcc atgccactga gcttacaggt ctttatcggg cgatagtgct
      481 ttattcttcg gggctttgtg gatgattttt cgaaatgttc atgatagtta ggtaaagcta
      541 ccgcatatca tttgcaaaat gacagataga tatacttcaa tttgaatctt aacaattagt
      601 atgggaatgc tgtcaatcct ctgtatgcgt ccaccacata ttgaaatttt tgttctctaa
      661 tatattccac gggcgtcaag agtgtgaggc ctgtgctgtt atacagcacc agacgttgtc
      721 ccccatcttt gacctgtctc ttgatcagat ctcctgatca agagacttca tcaccaggta
      781 accctcagcc atatcctgat gtctgaacca gccatcccac ataactaccc aaccggggct
      841 gcttgtgcgt ttgctgtcat gccatcgccc cagtttagcc agtttcaaac aggcccattt
      901 cagggtcggt gtttgcgctg gaagctgctt tccttccagc ttaacccaca gtagtttcca
      961 ctctgtcggc gtcagtattt tctcacagct gtcattttgt gtttcttcac tgatacttcc
     1021 ctgccgcagg cccagcaccc gcaccgcgat aaccaccatg cgctcaaggt tatcccgggt
     1081 ctgcattcgc agcgattcca cacatgtacc accacttttc cacgctttgt ggtattcctc
     1141 tatcagccag cgttgctcgt aatggctgac gatacgtcgc gcatcggcgg cactcgccac
     1201 tttttctgat gtcagcggat accagcaggc tccgtcctct cctgctcccc gcaacagaca
     1261 tacgtgagcg agagcgcctg gccgctgttg tcgggatttt ttatgctgac ttcgctgtaa
     1321 ctgatgagca tccgggcctg gcgggctgcc cgcccgcctt tttgcatcac attcagcgtg
     1381 tggcttcccg cggctgactt tataatgcag gtaatgccag atatccgctt cccggtcaca
     1441 gacagtgatt acctgtttct gtatctcccc cagccgttcg gccatgcgct ccgaagcctg
     1501 ctgccagcgg taactttctt tttctgtcac gaacggtgca atagtggttc ttccgcaact
     1561 tgggtggaag aaaatggatg aaactttctg gtgaacaaag atctaagctg ttttaagtca
     1621 tgggcaactc aatgcactct cttaagacac ttctacagtt accttgcgga tggcgatgca
     1681 gtcgacaaat tattagctct gacggtataa ccctccatct ccacggaaaa cgcaaaaaag
     1741 cacaatgtcc tgaatgcttt aagcgtagcg actctgttca tagttgtcgt cggcgccgga
     1801 tacagcatct accctgctca ggacatacgc tatggcttgt attttccgtc cgccactggt
     1861 actgccgtaa cccggcttgt tcacgtaaaa tttttgccgg gtcgcttgct cccttcgccg
     1921 gttcacacca acagtcttca caggcgttac aaaatttaca acgtcaactg ggattaatcg
     1981 ccggaggtga ggccgggaaa cgggcagcaa cggcagtggg gctccgttgc agtgctgata
     2041 ctctccttcg cagggttatc aataccccgg agacgaaaca gtcaggcgcg cctcatgtcg
     2101 gtattgatga gtgggcgtgg catcggggcc accgttacgg tacgttaatc gtcaatcttg
     2161 atactcaccg tcccctcgtc ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt
     2221 tcagaaaata tccggaaata caaattgtct cgcgtgatcg cagtggagtc tatgcgacag
     2281 cagcacgtga aggtgcacct caggccagac aggtggccga tcgatggcat ctgctaaaaa
     2341 atattggcga tgcgcttgaa cgaatgatgt acagacatat acctctgata cgtcttgttg
     2401 ccagtgagtt gtcaccaaag aaatcacctg atccagaacc atctgtgcct gcagcatcgc
     2461 tccgtcgtcc ggaacgccta aaacaacaaa cccgcaaaaa acggcatcag cgttggacag
     2521 aggttatggc cctgcataac aaggaatgta gtttcaggga aatatcccgt attacaggcc
     2581 tgtcgcgtgt gacagtcagt cgctgggtgc gttcaggaac attccctgaa atgtcaaccc
     2641 gacctccaaa gcgagggctt ctggacccat ggagggagtg gttaaaagag caacgagaaa
     2701 gtagtaatta taacgccagc cggatatggc gggaaatggt ggcccgggga tttacaggca
     2761 gtgaaaccat cgtcagggat gcggttgcca aatggcgtaa aggctggaac ccaccggtta
     2821 ctactgccgt cagacttcct tcagtgtccc gggtaagccg ctggttgatg ccctggagaa
     2881 taaccaggga tgaagaaaat tatgcttccc gatttattag tctgatgtgt gaaaaagaac
     2941 ctgagctgaa aatagcgcag caactggcac tcgagttcta ccgtattctg aaaacccaaa
     3001 ataaatcaca gctaagcagt tggttcaatc gagtccacga aagcggttca gcagaatttc
     3061 ggcgcgtggc ggcggggatg gaagctgatg ctgcagctat atgtgaggca atcagtagtc
     3121 gctggagtaa tggtgttgtc gaaggtcatg taaatcgcct gaagatgctg aaacgccaga
     3181 tgtatggtcg agcaggattt gaactactca ggcagagggt aatgagtcca ctggcatgaa
     3241 aatctccacc caggttgcgg aagagccact ttccgtcgtt gacactgacg acatcttcct
     3301 gctgaaacag cgcctggccg aacaggaagc gctgatccac gccctgcagg aaaagctgag
     3361 caaccgggag cgcgaaatag accatctgca ggcgcagctg gataaactcc gccggatgaa
     3421 cttcggcagt cgttccgaaa aagtctcccg ccgtatcgca caaatggaag ccgatctgaa
     3481 ccggcttcat aaagagagcg atacgctg
//
LOCUS       DAZFDP010000292         3458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFDP010000292 DAZFDP010000000
VERSION     DAZFDP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..1173
                     /locus_tag="ACHJ1G_005169"
     CDS             91..1173
                     /locus_tag="ACHJ1G_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO4766836.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1362..1745)
                     /gene="quuD"
                     /locus_tag="ACHJ1G_005170"
     CDS             complement(1362..1745)
                     /gene="quuD"
                     /locus_tag="ACHJ1G_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO4766837.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1831..1971)
                     /locus_tag="ACHJ1G_005171"
     CDS             complement(1831..1971)
                     /locus_tag="ACHJ1G_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4766838.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1968..2330)
                     /gene="rusA"
                     /locus_tag="ACHJ1G_005172"
     CDS             complement(1968..2330)
                     /gene="rusA"
                     /locus_tag="ACHJ1G_005172"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4766839.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2327..2617)
                     /locus_tag="ACHJ1G_005173"
     CDS             complement(2327..2617)
                     /locus_tag="ACHJ1G_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4766840.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2610..2780)
                     /locus_tag="ACHJ1G_005174"
     CDS             complement(2610..2780)
                     /locus_tag="ACHJ1G_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4766841.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2780..3235)
                     /gene="ybcN"
                     /locus_tag="ACHJ1G_005175"
     CDS             complement(2780..3235)
                     /gene="ybcN"
                     /locus_tag="ACHJ1G_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO4766842.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3232..3333)
                     /locus_tag="ACHJ1G_005176"
     CDS             complement(3232..3333)
                     /locus_tag="ACHJ1G_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766843.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
BASE COUNT          925 a          820 c          763 g          950 t
ORIGIN      
        1 ttacatcttg aaataatcac attgattaga tgaatattta tcgcgcagtg acatcatttt
       61 ttaataatag ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc aatttctgct
      121 gtagctgcat cagtactgat ggcgatgtct gctcaggcag ctgaaattta taataaagac
      181 agtaacaagc tggatctgta cgggaaagtt aatgctaagc actacttctc ctctaatgat
      241 gcagatgatg gtgatactac ttatgcccgt cttggcttca aaggtgaaac ccaaatcaac
      301 gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg cgctgaatct
      361 caaggttcct ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac
      421 ggctccatcg attatggccg taactacggt gtagcatacg atattggtgc atggaccgac
      481 gttctgccag aattcggtgg cgatacctgg acccaaacag atgtattcat gactggtcgc
      541 accacaggtg ttgcaactta tcgtaacaat gacttctttg gtctggttga tggtctgaac
      601 tttgctgctc agtatcaggg taaaaatgac cgtactgacg taactcaagc taatggtgat
      661 ggtttcggtt tctccactac ttatgagtat gaaggtttcg gcgtaggtgc aacctatgct
      721 aaatctgatc gcactaataa tcaggttatc tacggtaaca acagcctgaa tgcatctggt
      781 caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct
      841 actacctatt ctgaaaccca aaacatgact gtttttggta aaaaccatat tgccaacaaa
      901 gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt
      961 gcttatctgc aatctaaagg gaaagacttg ggtgcttggg gtgatcagga cctggttgaa
     1021 tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgactac
     1081 aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat
     1141 atcgttgctg taggtatggt ttaccagttc taatttgatt actaaaacat atgttgtggg
     1201 aggctttgcc tccccaacat ataagtggct ccctcaagcc acttccttta gaagcacaac
     1261 cttgcttcta actatataaa ccttctgtta tatattaccc tttatttttg ggggcgtttc
     1321 aacgccccat tcttaataat ttttagtaaa caattggcat attaattaga gttattaaca
     1381 acgatatcca tctctaaccg gatatctaat gccattaaca tcccttcaat tatgccctca
     1441 gccttctgta accttttccc gatataacca tcagagcagc aatgcttacc tgccagtgac
     1501 atgaatgtca taccgactac ataataatct actaataaat cgtgcaaatc gctgttgttc
     1561 tttttcagac gggccatgca cccgcaaatg atcatcgcgt catcgtcaca acattgcggg
     1621 cgagatttta cttttgaagt aattaatccc ttaaaaccgg cggcaatgga cgaccaggtc
     1681 acatcttcat gattattagc cgcccacgct ccccaacgct caagaaccat ctgaatatca
     1741 cgcatcaact tactccacaa aaatcagacc agaacgccaa ttacaagcaa aaatcaacga
     1801 aacagtatta gttgattgtt atctctgact tcatactcct gctcctgtca gggttttggc
     1861 gtaattcttc agtattcggt aatcggtcaa aacagaaccg gggaaacgat ataagcgcag
     1921 acgcccccag cggtggcgaa gacgttctgc catataaaac tcaaacatca ttcattcccc
     1981 atttcggtga tggtcagttc cagcctccca cctttggtaa caggcatctt cacaacgcgg
     2041 taatcaacga cctgagcatc atccagccag aaacctgctt tagtgagtgc gtcaaaagcg
     2101 gctttttgca gattatccag gtcacggcga cggcgatccg gcatgtggca ctcaatgcgg
     2161 attttcacag gcatagccaa gccgatatcc agcattgcgt ttttaatgat tcgggcgacg
     2221 ttatcgcggt atgcctgccc ctctgcgctg acgtgcgtgc gcccgcgatt atggcggtaa
     2281 tagcgattat tgctcggagg ccagggtaat gtgatgctgt aggtattcac gccttgatta
     2341 ccccctcttt cagccagata acctgcgttc tcgccatacc ttccagcgcg cattcttttg
     2401 cgtactcagc atcgacaaaa tgcgtgcggc ggtcgatttc gtcgtgacag gcagaacagg
     2461 caatggtggc aataaggtct ggcggtttaa taccggtgcc acacagtcca gccagccgga
     2521 tatgtgccag tacagacgtt tcagggttgc cattgcatac gccagggatt cttacctggc
     2581 attcgcgacc acgcgctgct tttctcaaat cagccatgat tcctccttgc tgccagtcgc
     2641 aaccattttt tatcaaccag gctggcggta tatccgagca gtgttggtat ttcggatggt
     2701 tttagctcag gcttacgctt acgacgattt ggtactctgt agatgtgtcc gttcatgaca
     2761 cgaataagcg gtgtagccat tacgcctcct gcttgtcgcg cagcagctgg aactcgcagc
     2821 tctgcggaat agtcaggtgg cagccaatat tcatcgccca ggcttcaacc ttacacagga
     2881 agacatacat ctctccggta tcaagatcgg aggtatggcg taacgactgg atagtagtga
     2941 tttcgccggt tacgacatca accaggtcct tggtttcata accgaggtat gtgtgtttga
     3001 gagcatcttt tacccatgct gcggtagcga acgatttccc cctgcggata aggtattcac
     3061 tgatttcgct gtaccacatg tggctaagtg cattctggga aagactgcgt ctctcgcgcc
     3121 acggtttaag caccatgcga aagcacttgc cgtcttccaa ataaggctgg agctgctgac
     3181 cgatagcggt gaagttaccg cgatgcagtt tgatgccgtc ttgtgggagg ttcacgcttc
     3241 acctccgcag aggtcaaacg ctggataaag aaaatcgcag gtgcatttct gcatctgtga
     3301 cgggagaaga tagtttagat tgtatgtgcg cataaacgtc cccgtttagc gcagaagtca
     3361 ccggaggagt tcaaactccg gtgacttaat tatgacaagt tgattattga aaatcaaaag
     3421 tcatttaaac aataatgata cccagcaaga gatttctt
//
LOCUS       DAZFDP010000293         3424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFDP010000293 DAZFDP010000000
VERSION     DAZFDP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1541
                     /locus_tag="ACHJ1G_005177"
     CDS             <1..1541
                     /locus_tag="ACHJ1G_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO4766844.1"
                     /translation="KAFMYPIALLSVCGMMLGLGSGLASDDMGKFIPVLAIPLIKTIL
                     DFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGFMAMHLGTNFYLKQH
                     DLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLRIPESLGFYSG
                     PRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPF
                     GLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMI
                     QYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIEFLFLFVSPLLFVFH
                     AFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAVCVEVAYFFIYYFVF
                     RWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAAEQMVKALGGKENIK
                     SLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNY
                     MGIC"
     gene            1541..2749
                     /locus_tag="ACHJ1G_005178"
     CDS             1541..2749
                     /locus_tag="ACHJ1G_005178"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO4766845.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2718..3314
                     /locus_tag="ACHJ1G_005179"
     CDS             2718..3314
                     /locus_tag="ACHJ1G_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO4766846.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
BASE COUNT          934 a          623 c          794 g         1073 t
ORIGIN      
        1 ggaaggcatt tatgtatccc atcgctctgt taagtgtatg tggtatgatg ttagggctgg
       61 gaagtggttt agccagcgat gatatgggaa aattcattcc agtgttggct attccgttaa
      121 ttaaaaccat acttgatttt attgttagcc ttggtttgtt tgccttcgtt aatttacctg
      181 tattgtttgc gatagctata cctttgggat tattaaaaga gaaagatgat aaagcctatg
      241 gcgctttttc tggcttaatt ggctttatgg cgatgcatct tggaacgaac ttttatctta
      301 aacagcacga cttattggta gctgctgacc aaatgtcgac acatgggcaa accatcatac
      361 tcgggatcca gtcctacaat accagcgtgt tgggtgggat tgtggcaggg ttattagtcg
      421 ccagcatgta taaaaagatc gttaacttac gtattcctga atcattaggg ttttatagtg
      481 gcccacgcct tgtgcctatc attacactga ttgtgatgag tggatttggt ctgttcattc
      541 cttttatctg gccgccattt ttcaatcttt tcatgctcat tggtcactgg atttcaactt
      601 ccggtcctgt tggttatttc ttctatgctg ttgccgaacg cgtgacgata ccttttggct
      661 taaatcactt ggtgacatca gttttccgct ttaccccaat cggcggatcg gcagtgattg
      721 gcggtgaaga gtattacggc accctgaaca tgtttatggc atacgtcaaa gagaatgcgg
      781 tcattccgct ggatttggcg gggaaaatgg agcagggcaa actgatgatt cagtatggtc
      841 tggctggagc cgcgctggcg atgtatcgca ctgctcatgc tcaaaacaga agggctatca
      901 aagcattgct gatttccggt gtgctcacgg taattattgg cggcgtcagc gagccgattg
      961 aatttctgtt cttatttgtc agcccgctgt tgtttgtctt ccatgccttt atgaatggat
     1021 tggctaacat ggttctgcca tatatgggcg tgaagatggg ctttactggc gatctgattc
     1081 aatttattag ctttggtgtc ttacgtggca caagaacggg atggccatta gcggtatgtg
     1141 ttgaagtggc ttatttcttc atttattact ttgtgttccg ttggaccatt ctcaaattta
     1201 acctgatgac tgtaggccgt gaagagtcca gtcctgtcac gctgaacgct cacgaagaaa
     1261 ctgcaatagc taacatccca gttcatgata aatcagagtt gcaggcagcg gagcaaatgg
     1321 ttaaggctct gggtggtaaa gaaaatatta aatcactgga taattgtgta actcgtttgc
     1381 gtttaactat cgcggatatg ggattgatag atgaggctgc aataaaaagg gccggaggaa
     1441 ttgcggttgt gaaacttgat caaaatacct tacaagtcat tatcggtaca aaagtcatcg
     1501 ccctacgtcg ggatatggat aattacatgg ggatatgttg atggatattt ttaatacctc
     1561 aatttctcgt aaaggaacat attgcacaca atgggacttt tgtgaagaca gatttggtgt
     1621 aaaagatgta ttaccattct ctatttcaga tatggatctt ccgataccgg aagctatcat
     1681 cagaacatta aaaaaacgtc tggaacaccc gattttaggg tacagtcgtt ggcagcatga
     1741 tgactatctg aattcgatag tcaattggta tcatcaacaa tataaaacag agattcaacc
     1801 agaatggatc acctatagcc caagcgtgat gtattcaata tctaaggcaa tagaaatatt
     1861 gacattgaat ggagataata ttctcatttt cacgcctgtt tataacgcat tttttgatgt
     1921 gattaagcat aatggtcgaa acatgctaac aacctctttg atgaaagaaa atgggagagg
     1981 ctacgttatc aactggcaag actttgagct taaaattaaa agcgcgaaga tggtgttatt
     2041 atgtaatcct cataacccga caggaacggt ctggtcggaa gacgatcttc acagaatcgc
     2101 agcatgctgt gcaaggcata atatttggtt atgttcagat gaaatacata gtgattttgt
     2161 ttttaatcat agtttcacgt cagcgctaac aataaaagat gacaaagtgg tggtgtttaa
     2221 ttcgatttcg aaaacattta atgttcctgc gctaaccgga tcatatatga tttcaactga
     2281 cgataatttt aatcataagt tcagaacgat atcgaggtat cgcgatttcg taaactcccc
     2341 atcagtactg aatgtaattg cgaccattgt tgcctataat gaatgtgaag aatggttaaa
     2401 gtcgttaaag gtacatattg cctctaacat tcaatttacc ttgcagtatt taaatgaaaa
     2461 tattccggaa ttgatcgtat ggccagctga gggctgctat ttttcgtggg ttgattgctc
     2521 ggcccttggt tatccctttg ataaatttta ccgtcgatta attaacgaag ggagagtagg
     2581 tattatggcg gggcatgttt atggtactga aggagagggc tatctgcgtc ttaatgtggc
     2641 ctgcagtcga gaaaaattat atatggggct tgcgcgcctt gcgagtgtaa ttaaaaatat
     2701 taatcaggaa aaagaaaatg aacaatacgg atcttatcca tcttattaaa cgttttatgc
     2761 gtaacgaaca taaagcagta gaagaggtta ttgattcccc cctttcggaa gtcgcgaatt
     2821 taattaaaat attacaatct tgccagggaa aagttgtttt tattggtgtt gggaaatctg
     2881 gcattattgc cagaaaactt gctgcaacat ttgccagcac cggaactccc tcgttttttg
     2941 ttcacggtac ggaagcggta cacggcgacc ttgggatggt ggcgaaagac gatgttgtta
     3001 ttcttatttc aaacagtggt gagacggcgg aaatcctggc gacactgcct agtttgaaaa
     3061 aaatgggtaa ttatttgatc tcttttaccc gcagtcatca ctcatcgctt gcgataagtt
     3121 gtgatttatc tgttgaaatc cccgtcaaaa gtgaggcgga taatttaggt ttagcaccgt
     3181 catgctcgtc tactgttgtt ctggttgttg gtgatgcggt ggcgcttgca ctctctgaat
     3241 tgaaaaaatt tactcgtgct gatttcggct tatatcatcc aggaggtgca ctcggcatta
     3301 aagcaaattc ataatttcaa agtataaata cagtaaccgg tcacgccatc ttttggggtg
     3361 gcgttcattg aagtgagtgt cagctatgaa aaatgttctg gtcaccgtcc ccagcttttc
     3421 agca
//
LOCUS       DAZFDP010000294         3410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFDP010000294 DAZFDP010000000
VERSION     DAZFDP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..485)
                     /locus_tag="ACHJ1G_005180"
     CDS             complement(<1..485)
                     /locus_tag="ACHJ1G_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO4766847.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TV"
     gene            complement(587..745)
                     /locus_tag="ACHJ1G_005181"
     CDS             complement(587..745)
                     /locus_tag="ACHJ1G_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766848.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHJ1G_005182"
     CDS             complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHJ1G_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4766849.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            complement(879..1010)
                     /locus_tag="ACHJ1G_005183"
     CDS             complement(879..1010)
                     /locus_tag="ACHJ1G_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO4766850.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(1035..2003)
                     /locus_tag="ACHJ1G_005184"
                     /pseudo
     CDS             complement(1035..2003)
                     /locus_tag="ACHJ1G_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            2259..2798
                     /locus_tag="ACHJ1G_005185"
     CDS             2259..2798
                     /locus_tag="ACHJ1G_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO4766851.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            complement(2822..3094)
                     /locus_tag="ACHJ1G_005186"
     CDS             complement(2822..3094)
                     /locus_tag="ACHJ1G_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4766852.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(3072..3291)
                     /locus_tag="ACHJ1G_005187"
                     /pseudo
     CDS             complement(3072..3291)
                     /locus_tag="ACHJ1G_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          754 a          781 c          724 g         1151 t
ORIGIN      
        1 acggtgtttt tctgcattgc tgcgtcagtg aatgctttta gaacctgttc aggtgtaggt
       61 gatggttttg attgctttgc tgctgcgttc tgctgatgtt tatgctcgtc ggtatctgca
      121 tctttcgcat catcaatgcc gaataaacca ttgaggcaat acttgcgtgc ataagagctt
      181 gtagctcccg taacttgtgc agaatccatt cctttcttgc tttcttcctc tcgtgcaaga
      241 gcggttgccg tatgactgtt ttcgccatcg gtaatagttg ccgtggcttt cacataatac
      301 cgatcaccaa tcaacacaac ttcatcgctg attgataaaa acaggccatt cagtaacggc
      361 ttaacgcctt caagaatatc ttcgcagctt ctgtatttat atttgccgaa tgagttgtac
      421 tgattctttg gcgcgttcag attctcctga atagctgcca gtcttgcgta aaattctttg
      481 ctcatatgat tgttctcaga atggacacgg cccaaggaaa taacgctgat ttaatacttc
      541 gactcgggac aaattaaggc atacccgcat tccttcgcgg tcaccattat ggcgatacca
      601 gagagctttc tgcgtgtaca tgcgcctctg taacttgctc tccttcactg tggttgcaag
      661 tgacatgaat atctccttcg ttaccgatta attctttcat ctgacgaatg aattcttcgt
      721 ctgaccagtt atctgtaaaa ctcatttcct gcgataacac ggaaggttga tagctgattt
      781 catcgcttta tttgcttcaa gccacatttt tgaatcacca ataaatcggg ctattactgc
      841 cttgttctgt gcagcacgaa gcatctggtg attgatggct atttcattgc gcataataag
      901 acctcaactc ttttccatcc gtcacgtaat ttacgggtga ttcgttcaag taaagattca
      961 tttaattgga aggcacccat gcgagcgcct cccgcgattg cgtaaatcat gggtggttcc
     1021 ttatgttggt tttattagta ggttattttt gttgcgaata attcgccttt tacgatggct
     1081 gttatgatat ttttagcaac atcttctgat gcgccaacct tgataaggtc agcaagtatt
     1141 ttgttattta cttctttccg gtgagcttta tcctttgctc tacgctcttc ttcgtccttg
     1201 attctttttt cttctgctat tctggcttgc tcttttgctt cagcctcgcg caggattcgt
     1261 tctgcctcct cctgtgcttt acggcgttct gcttcaattg ccgcatgctt ttctctttca
     1321 gctcgttctg ctgcctcttt tgcttcgcgc tgtgctcgtt gctcggcttc aatgcgttca
     1381 cgctctgcac gttcctctgc ggccttagct tctgcttctc gccttgctgc tgcttcaatt
     1441 tcggcttttg cctttgcttc ggcttcagct ctggctttct cttcagcttc tctttttaag
     1501 cgttcttcat gctctcgctt ttcctgctcc gctttgagtc ttgcctcttc tctttggcgg
     1561 tcaaattcgc gatccatcaa aatcgctatt tcatggtcag actcaatttg ctttgcgaga
     1621 gcttcagctg ctgccttagc ttcttcttcg gctttaatcc gcgcctgttc ttcctcataa
     1681 tcagtaagag gctggcgcgc cttggctttc agttcatcaa ggcgatcgcg cactgtcttg
     1741 cggttagcat caattagctt tggaatttcc ttcagttcag caacaaggtc tttgccaaga
     1801 ccatcgagat atgttttcgt ctgcgcaact ttatacgcca gataagcgat ctcctttctg
     1861 ccctttgccg ttgtgatatc aggcacaaag gacataactt cacgttcaac cttttgaaga
     1921 atttcttcaa tctggtcggc agactaaatt acagtcattg catttgcttt ttcaataaca
     1981 actaaatctg ttacttcact catatatcct ccgtcaaaaa aattgccctc acactggagg
     2041 gcaaagaaga tttccaataa tcagaacaag tcggctcctg tttagttacg agcgacattg
     2101 ctccgtgtat tcactcgttg gaatgaatac acagtgcagt gtttattcgt atgcctgtct
     2161 tttaaccaca tcaggctcgg tggttctcgt gtacccctac agcgagaaat cggataaact
     2221 ctattcaccc ctacagagag aatgatggag attcaccgat gagtaactgg tggcaggaac
     2281 tattacgttt cttcctgcgt ggtcttacgc tacaacagtt aattcatatg cttattattt
     2341 taattgcctt gataataatc accccagcat caattaaaga gtgggtagat ataaggaacc
     2401 ccgaaatact tccagatcac tggatgtatt acgcaatgct tttgtgtatc agttatgttc
     2461 tgaacagggg gatggagttt atatttctgg catcttcaga cagatataaa aaatatctca
     2521 gtaagagaga tgaggctaaa gtaattgtgg agactgagcg cctattcaat tctctgagta
     2581 ttcaggaaaa agaggtttta gcatttgctg ttatggcaaa taacaaaatc gtacttaagc
     2641 acggcgatcc ggtcgcttta tctcttatga gaaaaggcct tctccatcgc tcaggtgtga
     2701 cttacagcgc gtcaggtaaa gagaaatttg ttatacctga cgtctggttc catgagtgtt
     2761 atatgcgctt tgctggtaaa gctgatgagc taatttagtt cctctacggc gggggggatc
     2821 gtcacctcgc tgtcagttgt tttgatttcc ggtagcctgc cgcgtaaatg gctacgtttg
     2881 gaagacaagt tgaaccttca tattttctgg tcaacgttgt cagagttata acttctgctc
     2941 tcattgctgg tttgcgcttg cattgcaaga ccactcgtga aggggttggc ctgtgtagct
     3001 tgtcggagct aatcgcctcc tgactttgca ggtttgcgcg acgagctcta cggcgagaag
     3061 ctgcggtgcc tttaaattct gtttttctgg acatagattc ctcccgaata aactttggcg
     3121 atgcaatctc gaagctcctc ctgagacggt tacttcggca ttgcatccca cagcttatgt
     3181 ggttgggtga tctggctttt cagccacgta gtcgagtgtt cgacgttgtt taaagagcct
     3241 gccagtctgc tccgtttggc ttacagcgtc ctgctgatga gttgacttta tcgaaatgat
     3301 aaactgatgg caatagcaaa atgataaatt gtttgggttt tcaaatatcc tgttgattat
     3361 tatggttttt tatttgattg cgggaattta tcagaaggaa tgaagagact
//
LOCUS       DAZFDP010000295         3320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFDP010000295 DAZFDP010000000
VERSION     DAZFDP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            334..1650
                     /gene="shiA"
                     /locus_tag="ACHJ1G_005188"
     CDS             334..1650
                     /gene="shiA"
                     /locus_tag="ACHJ1G_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4766853.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1752..3206
                     /gene="amn"
                     /locus_tag="ACHJ1G_005189"
     CDS             1752..3206
                     /gene="amn"
                     /locus_tag="ACHJ1G_005189"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4766854.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          763 a          811 c          803 g          943 t
ORIGIN      
        1 acaatgatac taaaaacata actaacctcg cctccctact ggttataatg caatgcagtc
       61 tatcagaatc atcagggtgc aattttgtgc atatgcggac ttttatgttt catatctcga
      121 acctgtgggc cctctgctta atccttaaac aacaccagca actcctgcgc ttttatcttc
      181 catcgaattt ttcatgttgc cgctaatcag ccataaaatc atttgcagat gcgctctgtc
      241 gaggtagtct cataaggttc gtttatagat cgacggcaat gtgagttacc ttttccatac
      301 taattataaa aagacagtac aaacaggatc attatggact ccacgctcat ctccactcgt
      361 cccgatgaag ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg cagtttcgct
      421 ggtgccgtcg tcgactggta tgatttttta ctctatggca ttaccgccgc actggtgttt
      481 aatcgcgagt ttttcccgca agtaagcccg gcgatgggaa cgctcgccgc atttgccacc
      541 tttggtgtcg gcttcctttt ccgaccgctc ggcggtgtca ttttcggtca ctttggtgac
      601 cggctgggac gtaagcgcat gttaatgctg accgtctgga tgatgggcat cgcgacagcc
      661 ttgattggta ttcttccttc attctcgacc attgggtggt gggcacctat tttgctggtg
      721 acactgcgtg ccattcaggg atttgccgtc ggcggcgaat ggggaggcgc agcgttgctt
      781 tccgttgaaa gtgcgccgaa aaataaaaaa gccttttaca gtagcggtgt acaagttggc
      841 tacggagtag gtttactgct ttcaaccgga ctggtttcat tgatcagtat gatgacgact
      901 gacgtacagt ttttaagctg gggctggcgc attcctttcc tgtttagcat cgtactggtt
      961 ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga acaacagcaa
     1021 cataatcaag cggccgcgaa aaaacgcatc ccggttatcg aagctctgtt acgacatccc
     1081 ggtgctttcc tgaagattat tgcgctacga ctgtgcgagt tgctgacgat gtacatcgtt
     1141 accgcctttg cacttaatta ttcaacccag aatatggggt taccgcgcga acttttcctt
     1201 aatattggtt tgctggtagg tggattaagc tgcctgacaa ttccctgttt tgccaggctt
     1261 gccgatcgtt ttggtcgccg cagggtttat atcacaggtg cgttaatcgg aatgttgagc
     1321 gcatttcctt tctttatggc gcttgaagca caatctattt tctggatagt tttcttctcc
     1381 ataatgctgg caaacattgc gcatgacatg gtggtgtgtg tgcaacaacc gatgtttacc
     1441 gaaatgtttg gtgccagtta tcgctatagt ggcgctggag tcggttatca ggttgccagt
     1501 gtggttggtg gtggatttac accttttatt gccgctgcac tcatcactta ctttgccggg
     1561 aactggcata gcgtcgccat ttatttgctg gctggatgcc tgatttccgc aataaccgct
     1621 ttgttgatga aagacaatca acgcgcttga tagcctggcg aagatcatcc gatcttcgcc
     1681 ttacactttt gtttcacatt cctgtgacat actatcggat gtgcggtaat tgtatggaac
     1741 aggagacaca catgaataat aagggctccg gtctgacccc agctcaggca ctggataaac
     1801 tcgacgcgct gtatgagcaa tctgtagttg ctttacgcaa cgccattggc aactatatta
     1861 caagtggcga attacctgat gaaaacgccc gcaaacaagg tctttttgtc tatccatcac
     1921 tgaccgtaac ctgggacggt agcacaacca atccccctaa aacgcgcgca tttggtcgtt
     1981 ttactcacgc aggcagctac accaccacga ttactcgccc tactctcttt cgttcgtatc
     2041 tcaatgaaca acttacgttg ctgtatcagg attatggtgc gcatatctcg gtgcaaccct
     2101 cgcagcatga aatcccttat ccttatgtca ttgatggctc tgaattgaca cttgatcgct
     2161 caatgagcgc tgggttaact cgctatttcc cgacaacaga actggcgcaa attggcgatg
     2221 aaactgcaga cggcatttat catccaactg aattctcccc gctatcgcat tttgatgcgc
     2281 gccgcgtcga tttttccctc gcgcggttgc gccattatac cggtacgcca gttgaacatt
     2341 ttcagccgtt cgtcttgttt accaactaca cacgttatgt ggatgaattc gttcgttggg
     2401 gatgcagcca gatcctcgat cctgatagtc cctacattgc cctttcttgt gctggcggga
     2461 actggatcac cgccgaaacc gaagcgccag aagaagccat ttccgacctt gcatggaaaa
     2521 aacaccagat gcccgcgtgg catttaatta ccgccgatgg tcagggtatt actctggtga
     2581 atattggcgt gggaccgtca aatgctaaaa ccatctgcga tcatctggca gtactacgcc
     2641 cggatgtctg gttgatgatt ggtcactgtg gtggcttacg tgaaagccag gccattggcg
     2701 attatgtact tgcacatgct tatttacgcg atgatcacgt tcttgatgcg gttctgccgc
     2761 ccgatattcc tattccaagc attgctgaag tgcaacgtgc gctttatgac gctaccaagt
     2821 tagtcagcgg caggcccggt gaggaagtca aacagcggct gcgtacaggt actgtggtaa
     2881 ccacagatga caggaactgg gaattacgtt actcagcttc tgcacttcgt tttaacttaa
     2941 gccgggccgt ggcaattgat atggaaagtg caaccattgc cgcgcaagga tatcgtttcc
     3001 gcgtgccata cgggacacta ctgtgtgttt cagataaacc gttgcatggc gagattaaac
     3061 ttcccggcca ggctaaccgt ttttatgaag gcgctatttc cgaacacctg caaattggca
     3121 ttcgggcaat cgatttgctg cgcgcagaag gcgaccgact gcattcacgt aaattacgaa
     3181 cctttaatga gccgccgttc cgataataag ttctgaacct tcttatcaaa aatagatgtg
     3241 aaggattaag taagacatct ggcaaatagc ccgcaataaa cgcgggctgt ttgcttcctt
     3301 gggcggatac gagttttatt
//
LOCUS       DAZFDP010000296         3299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFDP010000296 DAZFDP010000000
VERSION     DAZFDP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHJ1G_005190"
     CDS             complement(<1..305)
                     /locus_tag="ACHJ1G_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766855.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     "
     gene            complement(302..2881)
                     /locus_tag="ACHJ1G_005191"
     CDS             complement(302..2881)
                     /locus_tag="ACHJ1G_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766856.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQS
                     WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSG
                     GRR"
     gene            complement(2862..>3299)
                     /locus_tag="ACHJ1G_005192"
     CDS             complement(2862..>3299)
                     /locus_tag="ACHJ1G_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4766857.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          641 a         1124 c          806 g          728 t
ORIGIN      
        1 gcactgaatt caacccgcag catgcgaacc cgtgatgacc aggcggcaca ggtcaccttt
       61 atctgccgcc aggcataagg cggtgtccac agaaacgcct tccagccacc gtgctctgcc
      121 aggaatgcct ccagatgtcg ggcctcctcc cgggtcacgg aaagcgtcac acggtatgtt
      181 ttcaggtcag cattcagccc cgccgccata cgctgcgaat acccgtcacc aaaacgcact
      241 tcacgcaccg atggctgcga gttcacctcc atatccggct tcactttcca gcgaaatgtt
      301 ttcatcgcct gcctccggaa aagacgccgc catcacgcat ctgcgcctga atctcatcct
      361 gcgccccctt gcgggccatg tcatacaccg ccttcatcag ctgcggcccc gcctgtccgt
      421 tggtgccgtc gttctgaatc accacgtgat tgttctgatt aaaactaatg ccttccgccc
      481 gccgcatctg cgccggactt ccggcaccgc ccacataacc accttccgca tacccgcgca
      541 tcagacgata caggttgccg acgccaatcc ggctggttgc ctccttcgtg aagacaaact
      601 ccccgcggtg gacaataccg gcaggttcgt atttgccacc ggttcccgta aatcctccgg
      661 tcgcgaaatg gaagttcgcc gccgcagcct gaatggccgt ccccgtggag gcagacgccc
      721 caccaccgaa agcaccaccc atggcgctgc caatactccc gacaatcccc accatggcct
      781 gcttcagaaa aatctctgtc agcatggaga gcacagaacg ggtgaaacca cgccagctct
      841 gttcgctgcc ggtcagcatc gctgccatat tctgtgcaat accgtcaaag gtctgcgtgg
      901 ccacgctttt aacctgcgaa aaactgtccg tcgcactttc tgcccactcg ccccagccgg
      961 acttcagacc ggccatccag cttccacgaa gctgctcctc cgcagaccag gtgttcttca
     1021 gtgcagatgt ggccttcgcc agcgcatccg gattatcacc gtacacgtca cgaaggcgct
     1081 gctcttccga ctcccgctgc acctgacggt cggtgaggcc ccgcgcctgg gcactgattg
     1141 ccgcctgctt cgcgccctgc tgctgctcaa accgcgcagc ctgctgtgcc agctcattca
     1201 gccgtttctg gtgttcaact ttgtctccca gctcagccag ctggcgtttg tactccagcg
     1261 tctctttctc atgggtcagc agggattttt cctgctcaga taactgccgt ttcgtggctg
     1321 cctctttcag gaccgcatac tgattttccg ctttccataa atcgcgacgc tgctggctga
     1381 ttttcccatt cgcaccgctg tgtttttcca gcgtccggag ctcggtttca agcgccagca
     1441 gggcagcatg cgcctggtct tcctgacgct caccggctga cactttgact cctgacgact
     1501 tcggcttttt cagcgtcgat tcataatcct ttttcgccgc cgccatcagc gtgttgtaat
     1561 ccgcctgcag gattttcccg tctttcaggg ccttattcag ttcttcctga cgggcggtat
     1621 atttctccag cggcgtcagc agacgctcat acgccttctg cgcctctccg gtatacttca
     1681 gctgtgatgc gtcccgttcg gcccggtccc tggcggccag ttcaccggct ttttccatat
     1741 ccgactgcag cgtggccgct gccagcccca gacgggcatt ttcccggtca ttccatgcgc
     1801 cctgaaggtt ggcccggaaa gaggaggttt taccgcggcg ctggctccga ctctggtacc
     1861 actgccattt tttatccgcc tcatcaaatg ccttctgcgc actggcgagc atatccgctg
     1921 aggattccgg acgaccgata tccagaatgg catcccacat cgatttgaat gccttccctg
     1981 ttttatccgc ccaggtctcc agtgttccca tgttttcttt caggcgacgg gtctgctcat
     2041 caaagccttt cgtggcgata tcgttcgccg cctgcaatgc cccggcctcg tctccggaac
     2101 gctgcagctg tgcaacatac gcaatctgct ctgccgtcac gttacggaac tggcgcgcca
     2161 tcgccatcag tcccgacgtc gggtcagtgg tcagcttccc gaaggcttca gcgactttat
     2221 ccacctccac accggatgca gacgcaaaac gcgcgacact ctggttgatg gcatcaaact
     2281 gttcaccacc acgcacaccg gcattcacca gggctgccag tgactctctc gcctggttaa
     2341 acgtcagccc tgctgcctgc ccggctcttg agagagtcag catacgatcg gcagtcagtc
     2401 cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg gaatccccct
     2461 ggtaccaggc gtacgccagc gcacctgtcg ccaccgccag cgaggtgacc ccgaccatcg
     2521 gcagggtgat cgcaccggca agccccctga gcatggggat catcccgccg aaggagtcct
     2581 tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccaccg gcaagctgcg
     2641 tggcgatatc cgtaaactgt gcgggcaggg tgcgcatggc cgctttatac tgtccgacgg
     2701 aaatcccggc tttttgcgca gccagcgcct gacggctcag gccctgttca acagcagtgg
     2761 cggtttttct ggcgtcagta tccagtcctg aaaaatgacg ccttacccgg ctcatctgct
     2821 catcgaaacg gaccgcatcc agactcaggt caataataag atcaccaacc ggctgggaca
     2881 tatctcacac ctcccggaat ccccgctgaa gccatcatta atgcggcatc atccaccatg
     2941 acatccgcca catccgcaga cgataaaata tcgccccctc cgtccccacc gaaccggacg
     3001 cctccggcaa gtcctgccgc tttctgcatc agcattttgt cctcatccag cctctccacc
     3061 tgctcttcct catgccgggg gacaagcaga ctgaaatcag agggatgcat atccggatcg
     3121 caaaaaaaca ggctgagtac agcgtacatc agcccggaaa aatgcatatc cagttgggta
     3181 tcctgaaaat aatgcgtgcg gtaaaaatgt cgccagtcgg catattcggt ggatgtcatc
     3241 ccggcaagca tggcgcgcca gtcaggcctc cccatctctc gcgccagtct gagggcaaa
//
LOCUS       DAZFDP010000297         3271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFDP010000297 DAZFDP010000000
VERSION     DAZFDP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHJ1G_005193"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="ACHJ1G_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4766858.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005194"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005194"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4766859.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="ACHJ1G_005195"
     CDS             1254..1523
                     /locus_tag="ACHJ1G_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4766860.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="ACHJ1G_005196"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="ACHJ1G_005196"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4766861.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..3229
                     /gene="dsbA"
                     /locus_tag="ACHJ1G_005197"
     CDS             2603..3229
                     /gene="dsbA"
                     /locus_tag="ACHJ1G_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4766862.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          828 a          785 c          797 g          861 t
ORIGIN      
        1 gaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaaatgc cggatgcgac
       61 gctggcgcgt cttatccagc ctactcttgc ttatccgttc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt gatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg cctgtctatc actaattctt caggtcgatg
      241 tccggctcca tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaactgta gatccagctc
      361 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttgtttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaat aaatgcatct
      661 ttattatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttccagtaat aaaggctcaa ttgcgcggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcgtgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggctgctaa atcctcggga atataaggag tatcgcacgg acaaaacaaa
      901 aaccactcgc ctgcttcctg ctgcattact gaaagcattc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcttgcct gatagatttc ctgatggcga
     1021 ttggcattaa ccacgacgtg agagagctgc gtcataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggtgc caccaaaatc atcgtgaaat
     1561 gatatccttc atcattcgta atgttttccg gatgatggga tgaacaacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gatgctctat ttgagcaagg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagttg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     2041 ggtctgaatg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgatcgccgc cgttacagcg
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagctatt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct gcgacaaacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttacaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgtc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtcactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc agcatctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tcaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggcgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagcaata tggatgtttt
     3181 tgttcagcag tatgctgata cagtgaaata tctgtccgag aaaaaataat tcaatgtaaa
     3241 ttacataagg cccgtgaata ttcacgggct t
//
LOCUS       DAZFDP010000298         3227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFDP010000298 DAZFDP010000000
VERSION     DAZFDP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..901)
                     /locus_tag="ACHJ1G_005198"
     CDS             complement(119..901)
                     /locus_tag="ACHJ1G_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4766863.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(903..1316)
                     /locus_tag="ACHJ1G_005199"
     CDS             complement(903..1316)
                     /locus_tag="ACHJ1G_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766864.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1876..2106
                     /gene="vapB"
                     /locus_tag="ACHJ1G_005200"
     CDS             1876..2106
                     /gene="vapB"
                     /locus_tag="ACHJ1G_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO4766865.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2103..2519
                     /locus_tag="ACHJ1G_005201"
     CDS             2103..2519
                     /locus_tag="ACHJ1G_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO4766866.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2681..>3227)
                     /locus_tag="ACHJ1G_005202"
     CDS             complement(2681..>3227)
                     /locus_tag="ACHJ1G_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766867.1"
                     /translation="MQICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFH
                     RRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNL
                     YRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIM
                     ECDKLIALPPAANQSSVARIA"
BASE COUNT          774 a          858 c          853 g          742 t
ORIGIN      
        1 tccgtacaat gtgagcatac tgtgaagtgg ctttaacaat tatgaaataa tacaagagat
       61 cacatattgc agtggtctct tttttataga agaaatcaca attcataatt gtgacttatt
      121 atgcatgttt gtttttcagc attgtgaccg catcggactc aggcatcgaa aactgcaccc
      181 ggtgccgggc agcgacatcc agcgcaaaca ccttcgtgta gacctccgtt gagctgatgg
      241 acttatgccc catcagactc tgcaggacct tcagcggtat accggcatac agcatatgca
      301 tcgcatagga atggcggaac gtatgtggcg tgaccgggac agagaacgtc acaccatcag
      361 cggcagcggc ggcaaccgcc tccccaatcc aggtcctgac cgttctgtcc gtcacttccc
      421 agatccgcgc tttctctgtc ctgcctgttc gtttattacg ccgttccatg gggattttca
      481 atgtggccac catcgtctgc agctggctga cgtaccagga gtcggagagc ggaaccagcc
      541 ggtgagtctg ctgcccggcg ggcgttcttc ctgccgtcct ggcggctttt tcggtccgtt
      601 gtttcagggt cgcaagctgc acaaacggat acggaggcgt gagcgaaaaa tccccccgcg
      661 tcagcgccag tgcttcatta atgcgcgcac cggtgttcca cagtgtggcc agcagcatct
      721 tgcggcgcag atccgggacg taatggagca gggcgctcac ttcaggggcc agcaggtatt
      781 tgggcagttc atcatgaacc atcgacatct ggcggagtgc cagcgctgcc ggataatcga
      841 tggcaacagg aagctgagcg gcatcccggg catggtggat cggtgccggc aggtgctgca
      901 tatcagtgtt cactccacgg ggaaacgtcg ggcaggtttt ccacaacccg gtactttccc
      961 ggataacgga gctggttacc gccacgcgtc cagacggtga caacaaaacc ccctccctgg
     1021 ttgttttcgg ggagcaggcg atcgcactgc tcactcagca tgtggatcac gaacggcgca
     1081 tcgctgccgt cttcaaatat cagttccagc gcatcgcggc catgcagcgt tcccttactg
     1141 atgatgacgt actcagcccc ccgcatctcc ctgagtaaat gttttgccgc atccgggaca
     1201 agcagcctgg ctgcgccggc attccagctg aggtagacat atccggcctg cgcctgtaca
     1261 gactgccagt aatccgttct ggcgacagcc tgaccttcat tctcaataaa aaacatgggt
     1321 gactcctttt ttcttttccg ggcagggacg gtcatgcgat aacgtcatcc atcatcactg
     1381 ctcttaccgt cacgccatgt gataacatca tgccgttatc atcatcacca tgataacaag
     1441 catccatata accacgctgt taaattaccg gaggaccatt tccgacagat ccgatttttc
     1501 tgttattgac gggttttcgg aagtgtatca cgctgaggaa ataatcatat cgctgttttc
     1561 cgcccctgcg cgacgcatca caggtcaggg agatgacaca acatatagtg tcatcagcag
     1621 atgaatccgg caccacatgt tgtgatgacg ggaggtggcg ttgtgtgggg ggagaggaag
     1681 atcctgaaca accttttttc ggatctccga taaaaaagaa agaaatagag atatgctaaa
     1741 catatgcctg gcatctacat atgctaccgc tatacatatg ccacacatct gcctatacta
     1801 aacatatgca cacacccggt gctttgaata ttccggatat gtttcacata tccatatgct
     1861 cgggagagaa agaacatgag aaccgtatcg atatttaaaa atggcaacaa ccgcgccatc
     1921 cgtctgcctc gtgatctgga ttttgagggg gtgagcgagc tcgagatcgt ccgggaaggg
     1981 gacagcatca tcctgcgccc cgtccggcca acctggggat cattcctaga gtacgaaaaa
     2041 gccgatccag attttatggc ggagcgcgag gatgttgtca gcgacgaagg gcgatttaac
     2101 ctgtgaacaa gacttatatg cttgatacct gtatctgctc gttcatcatg cgtgaacagc
     2161 cagaagccgt cctgaagcgt ctggagcagg cggtgctacg cggccaccgc atagtggtct
     2221 cggccatcac ctactctgaa atgcgcttcg gggctaccgg cccaaaggcc tcaccacgtc
     2281 acgtccaact ggtcgataaa ttctgcgccc gcctcgatgc catcctgccc tgggatcgcg
     2341 ccgcagtaga cgccaccacg aagattaaag tggcactgcg gctggccggg acgccgatcg
     2401 gcccgaacga cacggcgatt gccgggcacg ccatcgccgc cggggcgata ctggtaacga
     2461 ataatacaag agaatttgag cgggtgcctg acctggtgct ggaagactgg gtgaagtaag
     2521 acactctttt caaagtcagg agataagggc accaagaaac agcccgtata cgggctgttt
     2581 taacagatat ctacagtaag cggtcctaaa ctgtagggta tcttcgaaga tcagactatc
     2641 agggagctaa agcctatctt ccggcggcaa aaacagtggc tcaagcgata cgggcaaccg
     2701 agctctggtt tgctgccggt ggcagggcga tgagcttgtc acattccata atcacgtact
     2761 ccgggatgag atcgaatctg gacggatcta gttgacagat gaactcattt tcaatgtggt
     2821 acgtttcgtt aacgaatttt cgtattaccg gatggtcctg atccagttcc agtaaacgga
     2881 atacctgcag tttttcatag ccgttacgac agcgacggta caggtttttt atttcatctg
     2941 ggctgctgac gagagcctga agtcgcgggt aatcaaaact gtccacatac tcccggatat
     3001 cccgcaaggc ctgctgaatt ttctcaggag ccattttggg gtagcccgag cccgcagcgg
     3061 caggctcgcg ataatccagc ggggcgacgc gtcgatggaa tagattggat agaagctgat
     3121 aagcatcacc acgttcgtca acgatttcaa agtagcgacg aagatagatg agcttaatga
     3181 tctcctcaca gtctgctgac gcagtgatgg atttgcagat ctgcatg
//
LOCUS       DAZFDP010000299         3164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFDP010000299 DAZFDP010000000
VERSION     DAZFDP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..421
                     /locus_tag="ACHJ1G_005203"
     CDS             101..421
                     /locus_tag="ACHJ1G_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO4766868.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            436..2769
                     /locus_tag="ACHJ1G_005204"
     CDS             436..2769
                     /locus_tag="ACHJ1G_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4766869.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRHLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT          719 a          928 c          902 g          615 t
ORIGIN      
        1 ttcgcgcagg gatacgcacg tcttcaggag acgcagatat gccttatcag ttaatgcaac
       61 cggcacggaa tgcagtcatc tgtcacaggg aggaaagcaa atgaaaacac ccttaccgcc
      121 cgtcttacgc gctgcccttt accgtcgcgc tgtcgcctgt gcctggctga ccgtgtgcga
      181 acgtcagcac cgctacccgc atctcaccct tgagtcactg gaggcggcca tcgccgctga
      241 gctggaaggc ttttatctgc gccagcacgg tgaggaaaaa gggcgtcaga tagcctgtgc
      301 cctgctggaa gatttaatgg aatccggccc cctgaaggcc gcgccgtcgc tgtcctttct
      361 cgggctggtt gtgatggatg aactctgtgc ccgtcacata aaagcgccgg tactgcactg
      421 aaggagaaca acaccatgaa aatgaacgta accgccaccg tcagccatgc gctcggccac
      481 tggccgcgta ttctcccggc gctggggatt caggtgctga agaaccatca tcagccctgt
      541 ccggtctgtg gcgggagtga ccgcttccgt tttgatgaca gggaggggcg cggcacctgg
      601 tactgcaatc agtgtggtgc cggtgacggc ctgaaactgg ttgaaaaggt gtttggtgtt
      661 tccccgtccg acgcggcagc aaaggtggct gccgtgaccg gcagcctgcc cccggctgac
      721 ccggcagtga cggccgccgc cgttgctgaa acagacgctg cccggaagaa cgccgccgca
      781 ctggcacaaa ccctgatggc gaaaacccgt cccggaaccg gtaacgccta cctgacccgc
      841 aagggctttc ccggccggga atgccggatg ctgaccggca cacacagagc cggtggcgtg
      901 agctggcgcg ccggtgacct tgtggtgcca ctgtatgacg acagcggcga actggttaac
      961 cttcagttaa tcagtgctga cggccgtaag cgcaccctga aaggcggaca ggtcaggggc
     1021 acctgtcaca cccttgaagg acagaatcag gccggaaaac gtctgtggat agcggaggga
     1081 tacgcgaccg cacttaccgt gcatcacctg accggtgaaa cggtaatggt ggcgctttct
     1141 tccgtgaacc tcctttctct ggccagcctt gcccggcaga agcatccggc ctgtcagatt
     1201 gtccttgccg cagaccgtga cctcagcggt gacggccaga aaaaagccgc cgcagccgca
     1261 gatgcgtgtg aaggtgttgt tgccctgccg ccggtcttcg gtgactggaa tgatgccttc
     1321 acgcagtacg gcggggaagc cacccgtaag gccatttatg atgccatccg gccaccggct
     1381 gaaagcccgt tcgacaccat gagcgaagcg gagttctccg ccatgagtac cagcgaaaag
     1441 gccatgcgta tctatgagca ttacggcgag gcgctcgcgg ttgatgccaa cggccagctt
     1501 ctgtcccgtt atgaaaatgg tgtctggaag gtgctgccac cacaggactt tgcccgggat
     1561 gtggccgggc tgtttcagcg gctgcgcgcg ccgttctcct ccgggaaggt ggcctccgtg
     1621 gttgacaccc tgaagctgat tattccgcag caggaagccc catcccgcca cctgattggc
     1681 tttcgtaacg gcgtgctcga cacgcagaac ggcacgttcc acccgcacag tccgtcacac
     1741 tggatgcgca ccctgtgcga tgtggatttc accccgccgg tggacggtga aacgctggaa
     1801 acccacgctc ccgcgttctg gcgctggctt gaccgtgccg ccggtggccg tgcggaaaaa
     1861 cgcgacgtga ttctggctgc actgtttatg gtgctggcaa accgctacga ctggcagctc
     1921 tttctggagg tgaccggtcc cggcggcagc ggcaaaagta tcatggccga aatagccacc
     1981 ctgctggccg gggaggataa cgccacgtcg gccaccatcg agacgctgga atccccgcgt
     2041 gaacgtgccg cgttaactgg cttctcactg atacgcctgc cggatcagga aaaatggagc
     2101 ggcgacggtg ccggactcaa ggccatcacc ggcggcgatg cggtgtccgt ggacccgaaa
     2161 taccgggatg cgtactccac gcatattccg gcggtaattc tggccgtgaa caataacccg
     2221 atgcgcttca ccgaccgcag cggcggcgtg tcacgcaggc gggtgattat tcacttcccg
     2281 gaacagatag ccccgcagga gcgcgacccg cagcttaagg acaaaatcac ccgcgagctg
     2341 gcggtcatcg tgcgtcacct gatgcagaag ttcagcgacc cgatgctcgc ccggtcactg
     2401 cttcagtccc agcagaactc agacgaggca ttgaacatca aacgggatgc cgacccgacg
     2461 tttgatttta tcggctatct ggaaaccctg ccgcagacca gcggcatgta tatggggaac
     2521 gccagtatca tcccgcgtaa ttaccgtaaa tacctctatc acgcctatct ggcctacatg
     2581 gaggcaaacg gctaccggaa tgtactcagt ctgaaaatgt tcgggctggg gctgccggtg
     2641 atgctgaagg aatacggact gaattacgag aagcgccata ccaaacaggg gatacagacc
     2701 aacctgacgc tgaaagagga aagctacggc gactggctgc caaaatgtga cgaccctgca
     2761 acaacctgac cctcatgact catctgaccg gcatctgccg gtcttttttc atccctgaat
     2821 cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg ttatcacact
     2881 gaaattaaaa gagaaaaacg aaaggtgaac agtgtgaaca atcaaatcaa aaaaaacttt
     2941 tttcttcctg agtgatttca gtgcggagga ttaatcaccg gtatgagtca caccggcaga
     3001 atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac ctatcaaact
     3061 gaaataaaag gagaaaacag aaaggtgaac agtgtgaaca gttcttccga aaaaaaattt
     3121 ttttcctgag ttaacgtgcg tgaagatacg tgtaacaatt aatc
//
LOCUS       DAZFDP010000300         3160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFDP010000300 DAZFDP010000000
VERSION     DAZFDP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..403
                     /locus_tag="ACHJ1G_005205"
     CDS             <1..403
                     /locus_tag="ACHJ1G_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4766870.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
     gene            433..3130
                     /locus_tag="ACHJ1G_005206"
                     /pseudo
     CDS             433..3130
                     /locus_tag="ACHJ1G_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          834 a          697 c          767 g          862 t
ORIGIN      
        1 gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga
       61 tgccctgcag atggcgctgt ggtggcgtaa gaggccccgg aacgttatcg ttcacacgga
      121 ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa ctgaagcggc ataatctgcg
      181 tggaagtatg agcgcaaaag gttgctgcta cgataatgcc tgcgtggaaa gcttctttca
      241 ttcgctgaaa gtggaatgta tccatggaga acactttatc agccgggaaa taatgcgggc
      301 aacggtattt aattatatcg aatgtgatta caatcggtgg cggcggcaca gtttgtgtgg
      361 cggcctcagt ccggaacaat ttgaaaacaa gaacctcgct taggcctgtg tccatattac
      421 gtgggtagga tcaactttac tgatggaaca acaaaccagt tgaacatcag caactccctg
      481 attcatggtt caatcacttc aatgctgcct atgggtgaaa gcagtgatgg tcattatgtc
      541 tatcagttct ctgaatacgg tactgatcgt gtaattgatg ataattggca tgatggtgat
      601 gtattcactt taaatattgc taattctaca attgatgacg attatgaagg tttatacttc
      661 actgattcat atctggacgg tgacgttacc aaatatacta acgaaacctt ccatacccca
      721 gctggtgaag gtgaagaata cgccggtttg tttgcaaacg gtggtgttgg ccttgggctg
      781 gcagtgaatc ttgatgtcga aagtaacatc aatatttcta acaactcccg cgttgctggt
      841 atcagcctga ctcagggtaa taccgtaaac aatacttaca ctactgaatc tcatacttgg
      901 gataacaata tctcagttac cgattccaca gtgacttcag gttctgtaac tactctggaa
      961 gacagtggtt tctatggtaa ctctgcggaa cctagtgatt actctggtaa cggcgaagct
     1021 aatgatgtag ctctgtattt cagtgatagt gcggcttcaa attactccat gaaaaacaat
     1081 gtttatttca gcaattctac tctgctgggt gatgttgttt tcgcaagtac tttcaacgca
     1141 aacttctatc ctcaaggtca tgacagtaat gccgatggcg tacttggaca ccaatggtgg
     1201 ctgggctgat gactcactta atgttgatga actgaatatc actctggata atggttctaa
     1261 gtgggtaggc tcagctacta catcagcaaa tgttgatgat gaatctactg cttcgactga
     1321 ctggtatgat gttacaggta atagcctgta tccgggcgtt gtaacagaag caaatgcttg
     1381 gggtcgtacg attgatgatc aagtattcca gagcggtgtg tttaacgtca ctctgaataa
     1441 tggttctgaa tggaataccg ttaacgcttc aaacattgat actttagctg tgaataatgg
     1501 ctctcaggtt aatgtgtctg aatctagctt gatttctgat agcatcgctc tgaccaatgg
     1561 ttcttctttg aacattggag atgaaggtta tgttggaact gatcatctga cagttgatag
     1621 ctacagtact gtagcgttgt ctagtgatga caattcaagt atctataatg gttattacac
     1681 tccgtcgctg ttagcaaata caataactgt gactaacggt ggtttactgg atattaatgt
     1741 agaccagttc gatgataatc cgttccagac tgatactttg gaactgacca gcggcaacat
     1801 cgctgaccat aacggcaacg tagtgtctgg tgtattcgat atccacagca gcgattatgt
     1861 tctgaacgct gatttggtga acgaccgtac ctgggatact tccaagtcta actacggcta
     1921 cggtattgtt gctatgaact ctgatggtca tctgactatc aacggtaacg gtgacatgaa
     1981 caacggcgac gaactggata acagctctgt tgacaacgtt gttgctgcaa ccggtaacta
     2041 caaagttcgt atcgacaacg caacaggcgc tggcgctatc gctgattaca aagataaaga
     2101 aattatctac gtaaacgacg tcaacaccaa cgcgaccttc tctgctgcta acaaagctga
     2161 cctgggtgca tacacctatc aggctgaaca gcgcggtaac accgttgttc tgcaacagat
     2221 ggagctgacc gactacgcta acatggcgct gagcatcccg tctgcgaaca ccaatatctg
     2281 gaacctggaa caagacaccg ttggtactcg tctgaccaac tctcgtcatg gcctggctga
     2341 taacggcggc gcatgggtaa gctacttcgg tggtaacttc aacggcgaca acggcaccat
     2401 caactatgat caggatgtta acggcatcat ggtcggtgtt gataccaaaa ttgacggtaa
     2461 caacgctaag tggatcgtcg gtgcggctgc aggcttcgct aaaggtgaca tgaatgaccg
     2521 ttctggtcag gtggatcaag acagccagac tgcctacatc tactcttctg ctcacttcgc
     2581 gaacaacgtc tttgttgatg gtagcttgag ctactctcac ttcaacaacg acctgtctgc
     2641 aaccatgagc aacggtactt acgttgacgg tagcaccaac tccgacgctt ggggcttcgg
     2701 tttgaaagcc ggttacgact tcaaactggg tgatgctggt tacgtgactc cttacggcag
     2761 cgtttctggt ctgttccagt ctggtgatga ctaccagctg agcaacgaca tgaaagttga
     2821 cggtcagtct tacgacagca tgcgttatga actgggtgta gatgcaggtt ataccttcac
     2881 ctacagcgaa gaccaggctc tgactccgta cttcaaactg gcttacgtct acgacgactc
     2941 taacaacgat aacgatgtga acggtgattc catcgataac ggtactgaag ggtctgcggt
     3001 acgtgttggt ctgggtactc agttcagctt caccaagaac ttcagcgcct ataccgatgc
     3061 taactacctc ggtggtggtg acgtagatca agactggtcc gcgaacgtgg gtgttaaata
     3121 tacctggtaa tattcttcac tccgaagaaa ttctggtaat
//
LOCUS       DAZFDP010000301         3136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFDP010000301 DAZFDP010000000
VERSION     DAZFDP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHJ1G_005207"
     CDS             <1..99
                     /locus_tag="ACHJ1G_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO4766871.1"
                     /translation="AKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            99..482
                     /locus_tag="ACHJ1G_005208"
     CDS             99..482
                     /locus_tag="ACHJ1G_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766872.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
     gene            506..799
                     /locus_tag="ACHJ1G_005209"
     CDS             506..799
                     /locus_tag="ACHJ1G_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO4766873.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            810..974
                     /locus_tag="ACHJ1G_005210"
                     /pseudo
     CDS             810..974
                     /locus_tag="ACHJ1G_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
     gene            971..1078
                     /locus_tag="ACHJ1G_005211"
                     /pseudo
     CDS             971..1078
                     /locus_tag="ACHJ1G_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1555..2223
                     /locus_tag="ACHJ1G_005212"
     CDS             1555..2223
                     /locus_tag="ACHJ1G_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4766874.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            2225..2413
                     /locus_tag="ACHJ1G_005213"
     CDS             2225..2413
                     /locus_tag="ACHJ1G_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766875.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            2417..3034
                     /locus_tag="ACHJ1G_005214"
     CDS             2417..3034
                     /locus_tag="ACHJ1G_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766876.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLSLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
BASE COUNT          946 a          649 c          824 g          717 t
ORIGIN      
        1 gccaaagcgt ggaagatgct cgaaggcaca ccggagcagc acaaagcgca ggacgtttac
       61 aacatcagac gagacgaatt agaaggagct gctgcttaat ggcacattcg attactgtaa
      121 gactaaacaa gcccgcaaga gagtttcagg ccggggaaaa tatcggattc aacatccgtg
      181 ctggcgttca gtattacgat cgccagacaa aaaagaaaga atggacaaac tacagcgccg
      241 ttgtatttgc caagccggga gcgcaagcgg attactaccg tagtgttctt gttgaaggtg
      301 gcattgtgga aattaccgga gaaaacatca gggttgatgt ttatcagggg caaaatggtc
      361 aatcaatcac tcttgaatta ctgaatgcaa agattggatt tgcagcttca ggaaatggcc
      421 cgcagcagca aagtagtaac cagcagaaca ctcctgtata cgacgattcc atcccattct
      481 gatttagaaa aataaggatt taattatgcc agcgcctctg tatggtgcgg atgacccacg
      541 ccgctgttcc ggcaattccg tatcggaggt gctggataaa ttcagaaaaa actacgatcg
      601 gataatgtcg ctaccgcagg aaacgaaaga ggaaaaggaa tttcgccatt gtatatggct
      661 tgcagagaaa gaagaacgcg agcgaattta ccagacatca atccgaccat tccgcaaagc
      721 cacatatacc cacttccctg aaattgaccc gcgcctgcgt aattaccgct cacgctatgg
      781 cgctatcagt aatgactgag gaatttacca tgagaggact tgcatacaat cccggcattc
      841 ttccggcaga aatgattatt cgccaacgcg taaagccaat gccatcgaga gagtaattgc
      901 ttaagagaaa gagtttcggt tctgttaatg acaacaaata tctgaatgct atgtggcgga
      961 gtgggaaaaa atgaaacaaa tgtcactaat tgagatggat ggttttctga aaggtaaatg
     1021 catcccacga gatctaaagg ttaacgaaac aaacgctgaa tatcttgtcc gtaagttcgg
     1081 tgaacttgaa tcaaaactgg aaacggcgtt gcgggagtgt cgttctgctg gaatcacgat
     1141 tgataacctt gaggccaagt gcgcggcgct ggcagcggag agtgcgggga tgaagaagtt
     1201 ctgcaaagac gctgcattcg atgccgatta cgaagcagag ctaggtatgg agcgtggttt
     1261 attcagtgat gcgcttaacg aaatcaaaac cccagccacc gatgctttcc tggctgaagt
     1321 acgtgcggaa gcacgcaacg agggtattaa ctatgccgca agccgtcttg ctgctgcttt
     1381 caaccacgga tttatcaata agtctttacg tgaagttttc gatgttacgc gcatgattct
     1441 gtcagcgaaa gaagagttgg ctaatgaacc gcatccgatt gatggcctgt ctggtgaata
     1501 tgcggagaaa tcccttgaag aatgggcgga acagattcgc aaaggaggca accagtgagc
     1561 ctgattgact atcaggcact gcgtgccaag gcagaaaaag caacgtgtgg tgtatggtcg
     1621 ctcgaatatg gagagagccg atttgattgt gatgatgcgt taattcatcg tgaagttgtt
     1681 ggatatcttc ccatttgcag aattgaagga gcacatccag aaagcggttt cgatgaagat
     1741 ttccaaatgg aacagcaggc caatgctgaa ttcatcgccg cagccaatcc ggctactgtg
     1801 ctggcattac tggatgaact ggaaagaaac cagcaataca tcaaacgccg cgatcaggag
     1861 aacgaggata ttgcgctaac ggtagggaaa ctgcgtgttg agctggaagg caaagacaag
     1921 ctgattgcag agcttggaaa acaatgcgcc gaatgggagc gaaaagcatt aagcaacttt
     1981 gaagagtgtg ctgcgatggc tgaacgtatc gaagagatga gtaagcaaag ttgcgaagcc
     2041 cgggagcgtg atttgtttga atcatgggta atgcattcaa tttgtatctc caaatcgacg
     2101 cttgaaggat tgcgtaccga aactggatac cgtaacgcaa ccttatcagg cacagacttc
     2161 aaccgaatgt ggaaacaatg gaaatctatc cgcgctactg gcattcgcat caaaggagag
     2221 tgagatgtcg ctgacggtta ggcagttaat cagcaagctc agaaaaatgc ctcctgaggc
     2281 tgtcgtggtc tggcagggct atgaccaatc agagggtgag tataacgact ttgtaggtca
     2341 tgttgctgat gttacagatg agaatgcacc atcttttgac ccagaagtgc gcgttgtggc
     2401 gctaagagga taacccatga ccactattac caaagagcga ctactgacaa tcaagcagtg
     2461 gcgcgaaaca tacggacctg gtagcaacgt tgtactgcca gcagaagaag cggaagaact
     2521 ggcacgaatt gctctggcat cgctggaagc agagcctatt ggtgaggttt cagagaagcg
     2581 actcagcctt gttatggatg gaacggtaga ccttggcggg aaatcaactt atcgcatcat
     2641 taagggagaa aaagcgatga agttgttgcc gctggggacg aagttttata ccgcaccgcc
     2701 agcgccgata gcgttggagg ccattgaaaa tgcaatagaa tacatccgca gtatcgcttt
     2761 tcacatcgat gaagacgatt accacggcaa acatattgcg tatttcatgc gacaagcatt
     2821 ggcctggctg gaagggcatt catgcagtga tgacagtcag ggcaaatccg ataattcacc
     2881 attgccgcgc taccaggtga tcgaattaac aatgctggtt aaacaattgg tcagccaact
     2941 gaaaaaagca aaacctgatt gcaaattacc tgatagggcg atggattatc ttttgcgaaa
     3001 cggactggta agtgcggagg atgttttacg atgaccattt ctacaaaaaa gcctctttat
     3061 gctgaggttc acaatgttcc tgatgattat gagttcactg acgaggaact aaacagaatt
     3121 attgcaggtg atatgt
//
LOCUS       DAZFDP010000302         3130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFDP010000302 DAZFDP010000000
VERSION     DAZFDP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2037
                     /locus_tag="ACHJ1G_005215"
     CDS             <1..2037
                     /locus_tag="ACHJ1G_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4766877.1"
                     /translation="AGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIH
                     GIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYV
                     PYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAE
                     DYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQV
                     AGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYSKRGQEQISIS
                     QQLGNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDH
                     LLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYS
                     VQVGNTHGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGI
                     TFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLA
                     DNVELDETVITVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGS
                     IVAENGQVYLTGLPQSGKLQVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            2051..2581
                     /locus_tag="ACHJ1G_005216"
     CDS             2051..2581
                     /locus_tag="ACHJ1G_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766878.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2594..3097
                     /locus_tag="ACHJ1G_005217"
     CDS             2594..3097
                     /locus_tag="ACHJ1G_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4766879.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
BASE COUNT          899 a          672 c          722 g          837 t
ORIGIN      
        1 gcaggaaaat ccaactatgc atatttgaat ttacagagtg gcatcaacat tggtagttgg
       61 cgactgcgcg ataactcaac gtggagttat aacagtggga gtagcaattc atctgacagc
      121 aataaatggc agcatatcaa tacgtcggct gaacgtgaca ttattccctt acgctcacgg
      181 ttaacggtag gtgatagtta taccgatggc gatatttttg atagtgtgaa ctttcgtggc
      241 ctcaaaataa attcaacaga agcgatgttg cccgatagcc aacatggttt cgctccggtg
      301 attcatggta ttgcccgtgg aaccgcacaa gtgagtgtaa aacagaacgg atacgatgtt
      361 tatcagacta ctgttccacc cggccctttt actattgatg atatcaactc tgcggccaat
      421 ggtggtgatc tgcaagtaac cataaaagag gcagacggca gtattcagac attatatgtt
      481 ccttattcgt ctgttccggt tctccaacgt gctggatata cgcgttatgc gcttgccatg
      541 ggggaatatc gtagtggaaa taacctgcaa agctctccca agttcataca aggtagcttg
      601 atgcatggtc tggaaggaaa ctggacacct tatggcggaa tgcaaattgc agaagattat
      661 caggccttca accttggtat tgggaaagat ttaggacttt ttggtgcctt ttctttcgat
      721 atcacgcagg ccaatacgac acttgcagat ggcacccgtc acagtgggca atcggttaaa
      781 tccgtctaca gcaaatcctt ctaccagaca ggaaccaata tccaggtcgc aggatatcgc
      841 tattctacgc aaggttttta taacttatcc gacagtgcct acagtcgaat gagtggttac
      901 accgtcaagc ctcctaccgg agacaccaat gagcagacac aatttattga ttattttaat
      961 ctgttctaca gtaagcgtgg tcaggaacaa ataagcatct ctcagcagct tggaaattac
     1021 ggtacgacat ttttcagtgc cagtcgccaa agttactgga acacgtcacg cagcgaccag
     1081 caaatatcat ttggattaaa tgtgccgttt ggtgatatta cgacttcgct gaattacagc
     1141 tattccaata atatatggca aaacgatcgg gatcatttac tcgcttttac gcttaatgtt
     1201 cccttcagtc attggatgcg tacagacagt cagtcggcat ttcgtaattc aaacgccagt
     1261 tacagtatgt caaacgattt gaaaggcggc atgaccaatc tatcgggggt ttatggcact
     1321 ctgctgccgg ataataacct gaattatagc gttcaggtcg gtaacaccca cggaggtaat
     1381 acatcgtctg gcaccagtgg ttacagttct cttaattatc gtggagctta tggtaatact
     1441 aatgtcggtt acagtcggag tggtgacagc agccagattt attacggaat gagtggtggg
     1501 attattgctc atgctgatgg catcaccttt ggacagccgc tgggcgacac aatggttctg
     1561 gttaaggctc ctggtgctga taatgtcaaa atagagaacc agaccggaat tcataccgac
     1621 tggcgtggct atgccatatt accatttgcg acagaatata gagaaaaccg tgttgctctt
     1681 aacgcgaatt cccttgcaga taatgttgaa ctggatgaaa ccgtgatcac tgtcatccca
     1741 actcacggtg ctattgccag agcaacattt aatgcacaaa tcggcgggaa agtattaatg
     1801 acgttgaagt acggtaataa gagtgttcca ttcggtgcaa ttgtcacaca cggagagaat
     1861 aaaaatggca gcattgtcgc agaaaacggt caggtttatc tgactggact tccacagtca
     1921 gggaaattac aggtttcatg gggcaaagat aaaaactcaa actgtattgt cgagtacaag
     1981 cttcctgaag tttctcctgg taccttactg aaccagcaga cagcaatctg tcgctaagga
     2041 acaatagatt atgaaataca ataacattat tttcctcggt ttatgtctgg ggttaaccac
     2101 ctattctgct ttatccgcag atagcgttat taaaattagc gggcgcgtcc tcgattatgg
     2161 ctgcacagtc tcatcggatt cgcttaattt taccgtagat ctccaaaaaa acagtgccag
     2221 acaatttcca acgaccggta gcacaagtcc agccgtccct tttcagatta cgttaagtga
     2281 atgcagcaaa gggacaacgg gggttcgggt tgcatttaac ggtattgagg atgcagaaaa
     2341 taatactttg ttgaaactgg atgaaggaag caatacggcc tccggtttgg gtatagaaat
     2401 attggacgca aatatgcgtc cggtgaaact gaatgacctt catgccggga tgcagtggat
     2461 cccactggta ccagaacaga acaatatttt gccttactcc gctcgtctga agtcaactca
     2521 gaagtccgtc aatccgggac tggtgagggc ttcggcaacc tttacccttg aatttcaata
     2581 atcagagtca gatatgaaaa gacttcacaa gaggttcctg ttagctacgt tttgcgcgtt
     2641 attcacagca actctccagg ccgccgatgt cactatcact gttaatggtc gggtagtcgc
     2701 taaaccctgc actattcaaa ccaaagaagc taacgttaat ctcggggatc tttatacgcg
     2761 caatctgcaa caacctggtt ctgcatctgg ctggcacaat attactttgt cattaaccga
     2821 ttgtccggtt gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg acaatacggg
     2881 ttattacaaa aatgaaggta ctgccgaaaa tattcagata gagctgaggg atgaccagga
     2941 tgctgcgtta aaaaatggcg atagcaaaac ggttattgtt gatgagatca ctcgtaatgc
     3001 acagtttcca cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc agggaacgat
     3061 cgaggcgcta atcaatgtga tctacacctg gcaataacgt cgattcgatt ttcttagcct
     3121 tctttttaaa
//
LOCUS       DAZFDP010000303         3061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFDP010000303 DAZFDP010000000
VERSION     DAZFDP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1820
                     /locus_tag="ACHJ1G_005218"
     CDS             <1..1820
                     /locus_tag="ACHJ1G_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4766880.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHJ1G_005219"
     CDS             1817..2023
                     /locus_tag="ACHJ1G_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4766881.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..>3061
                     /locus_tag="ACHJ1G_005220"
     CDS             2020..>3061
                     /locus_tag="ACHJ1G_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766882.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDT"
BASE COUNT          726 a          781 c          928 g          626 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 g
//
LOCUS       DAZFDP010000304         3056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFDP010000304 DAZFDP010000000
VERSION     DAZFDP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..285
                     /locus_tag="ACHJ1G_005221"
     CDS             67..285
                     /locus_tag="ACHJ1G_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4766883.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            287..550
                     /locus_tag="ACHJ1G_005222"
     CDS             287..550
                     /locus_tag="ACHJ1G_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766884.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            561..686
                     /locus_tag="ACHJ1G_005223"
                     /pseudo
     CDS             561..686
                     /locus_tag="ACHJ1G_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            897..992
                     /locus_tag="ACHJ1G_005224"
                     /pseudo
     CDS             897..992
                     /locus_tag="ACHJ1G_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1242..1430
                     /locus_tag="ACHJ1G_005225"
     CDS             1242..1430
                     /locus_tag="ACHJ1G_005225"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4766885.1"
                     /translation="MPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WKPSHWMPLPEPPQEVRQ"
     gene            1546..1650
                     /locus_tag="ACHJ1G_005226"
     CDS             1546..1650
                     /locus_tag="ACHJ1G_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766886.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1897..2052
                     /gene="hokD"
                     /locus_tag="ACHJ1G_005227"
     CDS             1897..2052
                     /gene="hokD"
                     /locus_tag="ACHJ1G_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4766887.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2220..2498
                     /locus_tag="ACHJ1G_005228"
     CDS             2220..2498
                     /locus_tag="ACHJ1G_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766888.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2500..>3056
                     /locus_tag="ACHJ1G_005229"
     CDS             2500..>3056
                     /locus_tag="ACHJ1G_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766889.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          803 a          698 c          814 g          741 t
ORIGIN      
        1 tgcaaatgaa gccatgagtt accacaacga ttaatgtcgg gaggccgccc gaaagggcgg
       61 taagaaatga ctacattatt cagaaaagaa tatccgcaaa aaagtagggc gacagaattt
      121 ttgtttctca ttctgtttat cgtattgatg ataccgatat cccctctaat ttttgtctgg
      181 gcaatcggga aaataattga gccagttact gaattgtata ccgacgttgt atgggcgtcg
      241 ttcaacacac tgcacaataa aattaatccg tataaggaaa actgatatgg caactttgac
      301 aaaaaaagaa cgggcatggt taaacgaatt acaggaagtt cttgatcgct gtccatcacc
      361 gaaaaaaatt ggcttttaca ccattggcga taaaagcatt tacctgtatg acctacgccg
      421 catggatgaa atcatggagg ctcttgataa tcgttcgtcg atggattggt gtgttgctgt
      481 tcatgatatg aatgcagggt ttgatgaaaa gattttgttc ccctcatcag ttgaaagcac
      541 tgcgggttaa ggagtaacac atgaccacta ttaccaaaga acgtattgaa ttgttcatta
      601 aaaatccggt tgaaaacggg cttacccgtg gtgaacaaat ggaactggca cggattgcgc
      661 tggcatcgct ggaagcagag ccggtggcaa agattatagc tcattaccca ttaggggttg
      721 acgtaggcaa acaaaaattc gtacaggcca ttagagagct tcctgacttt ggcggatatc
      781 tatttgccgc ccctccagcg ccgatagtgc cggaagaaat gtattggcag gatgcgccag
      841 ttgaaggcag cagcaaagcg gctgcatacg ctacaggctg gaacgattgc cgcgaagcca
      901 tgcttcagtc cggaaacttt cgggaaaata aagattcgtc aaccaataat tttcggaaaa
      961 tcccggaagc gtcaaccagc tctccggtaa ctccggctct tctgcctggt ggtttcacca
     1021 ttgaggaggc gaaggaatta catgaagacc tggtacgcag ccacataagc aaggccttaa
     1081 gtggcgaaaa gatgaaaaag aaagatcgcg atgctgattt gcgctggatt catggcgtta
     1141 tagttcaggc agcgtggttt gtaaaagcat cactggagca gaatgcacta tcgggcaact
     1201 atccggtaac tccggatagt tggataagct gtagtgagcg aatgccggat accaaaacag
     1261 ccgttcttgt tgccgtggag tttgacagga aaggtgactg gcgaatgaaa tgggcgactt
     1321 acatcccggg tcatcctgac gctaatgatg ggtggataat tcctggtgcg tcgtggaaac
     1381 cgtcacactg gatgccgcta ccagaaccgc cgcaggaggt gcgccaatga actggcctga
     1441 agcatttgca attacaggcg ttgctatggc tatcgctttt ttagtatatg ttatttgtcg
     1501 gtgggggtaa aaacgttcgc cgggattaac accaaaggag ggaatatgtc ggatgatata
     1561 tcactggcaa tggaaggtgc gctggctgtt gttgctgttg tgggcgttta ctgcctggtt
     1621 gtgtttttga tggatcgact agggaactga attcattacg atatgggaat tcccatatcg
     1681 ggtaaaaacg gtttgcggga aaaggagagt taagtagaat tgctgcgggt gcttgaggct
     1741 atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt aacattacgc
     1801 gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa atgtaggtta
     1861 gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa ggcgatgtta
     1921 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
     1981 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     2041 cctgaggagt aagagacccg gcgggggaga aatccctcgc caccgctgat gtgtcaggca
     2101 tcctcaacgc acccgcactt aacccgcttc ggcgggtttt gttttttctg gtcgttctgg
     2161 tttacaatcc atccgtcagc ctgaacaact ggcacctgct gcgccagcag agacaaccga
     2221 tggcgcacga taccaaatta tacaattctg atgattctgc cgtctttgcc agcaggcgcg
     2281 gacggtgttt tcacgcattc aaatctgact ggtaccagca tcccccatgc actgaagaac
     2341 aggccgaatg gctcattcag tgttaccgca ggcgcggatg cgaggttaaa aaagccctta
     2401 gcctcgacta ccgtcactgg ataatctccg tcaggctccc ttactccgaa cggccaccgc
     2461 gtccgtcccg cacattccag caacggatct ggaggtaatg tgcgggtatt acttcgacct
     2521 gttctggtac cggaactcgg gctggttatc gttaagccag gccgtgaatc catgcgggta
     2581 tttcatggcg gcagggtgct ggtggagtct gaaccgaaaa gcatgcgtaa tctgccgtcc
     2641 ggggtcgttc ctgccgttcg ccagccgctg gcggaagata aatcattact gccatttttc
     2701 agcaatgagc gtgtgattcg tgctgctggt ggcgctggtg cactgtctga ctggttatta
     2761 cgtcacgtta aatcctgcca gtggcctcat ggtgattatc atcacagcga aactgtcata
     2821 catcgttacg gtaccggcgc gatggtgttg tgctggcact gcgacaacca gctgcgcgac
     2881 cagacatccg aatcacttga gcaacttgct caacaaaacc tgtcagcatg gatgattgac
     2941 gtcatccgcc acgcaatgaa tggcatacag gaacgggaat tatcgctggc tgaattatcc
     3001 tggtgggcag tctgcaatca ggtggtggac gcattacctg aggcagtatc gcgtcg
//
LOCUS       DAZFDP010000305         3047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFDP010000305 DAZFDP010000000
VERSION     DAZFDP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            442..1008
                     /locus_tag="ACHJ1G_005230"
     CDS             442..1008
                     /locus_tag="ACHJ1G_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4766890.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            983..1585
                     /locus_tag="ACHJ1G_005231"
     CDS             983..1585
                     /locus_tag="ACHJ1G_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4766891.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            1582..2247
                     /locus_tag="ACHJ1G_005232"
     CDS             1582..2247
                     /locus_tag="ACHJ1G_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO4766892.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2244..2867
                     /locus_tag="ACHJ1G_005233"
     CDS             2244..2867
                     /locus_tag="ACHJ1G_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4766893.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          935 a          607 c          732 g          773 t
ORIGIN      
        1 gtctaactat agttatcacc aaaaacttgc ctcgatttta gatttttcca gtatttgtag
       61 atattgcact gaacaccgaa tacgtagcag agggtgtcta cacgataacg ttctatgagc
      121 taccatgttg tcgaaaaatt gtttagtgag tatgacatca ggaatgtggt ggtctgtttt
      181 aatatttcta tttctatttc tatttctatt tctatttcta tttctatttc tatttctatt
      241 tctgtttgtt gtggtttttt ctttagttca tgtatttcga tttgtttctg aattatcggg
      301 ggtagtgtct tcccttttcc ctgaggctta tcatgcagtt attttcgcca tcttggtatt
      361 gtggaaatac cgacatccat agctttggtg agctctgttc aggtggccaa attcagaaaa
      421 ccattacgga ggaagaaggc gatggctaaa ccagcgcgaa gacgatgtaa ccgtaaaaga
      481 gaagatttaa ctgttaaaag gatatttgag ttactaagtt tcgataaatc taccggggta
      541 tttagatgga aagttcccac tcagggaagg atagcattaa atagtgttgc tggaactttt
      601 gattccaacg gttattcaat gatcatgata gatgggcgta gatataaaac tcacgtctta
      661 gttttttaca taactcataa tcgttggcct gctggtcaaa ttgaccacgt taatggaatt
      721 aggaccgaca ataggccaga aaatttaaga gaatgcctgc caatagaaaa ttcaagaaat
      781 ataaggatcc gaaagaatag caaatcaggt tgcagagggg ttacttggca caaacgacag
      841 aaaaaatgga atgttaggct aggattccat ggcaagagta aacacttcgg atgctttgat
      901 gatctggagt tagcggtact agttgctgaa gaagcccgag ataagtatta cggtgatttt
      961 tccggcaacg aaaggagcac ttatgcgaat ctatcgaagg aaatgtaaat gttgcaatga
     1021 atggtttata ccaaaatatc aaaatcaata ttggtgtaat gagatttgtg gaaccaagat
     1081 agcactcgaa cgacgaagta aagaacgcga aaaagcggaa aaagcagcag agaagaaact
     1141 acgacgagag gagcagaaac agaaagataa actgaagatt cgaaaactcg ccttaaagcc
     1201 ccgcagttac tggattaaac aagcccaaca agccgtaaac gccttcatca gagaaagaga
     1261 ccgcgactta ccatgtatct cgtgcggaac gctcacgtct gctcagtggg atgccggaca
     1321 ttaccggaca actgctgcgg cacctcaact ccgatttgat gaacgcaata ttcacaagca
     1381 atgcgtggtg tgcaaccagc acaaaagcgg aaatctcgtt ccgtatcgcg tcgaactgat
     1441 tagccgcatc gggcaggaag cagtagagga aatcgaatca aaccataacc gctatcgctg
     1501 gactgtcgaa gagtgcaggg ccatcaaggc ggagtatcaa cagaaactta aaaaactgcg
     1561 aaacagcaga agtgaggttg catgaatatc tacgaaagaa ttgatggcag caaataccga
     1621 aatatttggg tagttggcga tctgcacgga tgctacacga acctgatgaa aaaactggag
     1681 acgataggat tcgacaccaa aaaagacctg cttatctcgg tgggcgattt ggttgatcgc
     1741 ggtacagaga acgtagaatg cctggaatta atcacattcc cctggttcag agctgtacgt
     1801 ggaaaccatg agcaaatgat gattgatggc ttatcagagc gtggaaacgt caatcactgg
     1861 ctgcttaatg gcggtggctg gttctttaat ctcgattacg acaaagaaat tctggctaaa
     1921 gctcttgccc ataaagcaga tgaacttccg ttaatcatcg aactggtgag taaaggaaaa
     1981 aaatatgtca tctgccacgc cgattatcct tgtgacgaat acgagtttgg aaagccagtt
     2041 gatcatcagc aggtaatctg gaaccgcgaa cgaatcagca actcacaaga cgggatcgtg
     2101 aaagaaatca aaggcgcgga cacgttcatc tttggtcata cgccagcagt gaaaccactc
     2161 aaatttgcca accagatgta tatcgatact ggcgcagtgt tctgcggaaa cctcacattg
     2221 attcaggtac agggagaagg cgcatgagac tcgaaagcgt agctaaattt cattcgccaa
     2281 aaagcccgat gatgagcgat tcaccacggg ctacggcttc tgactctctt tctggtactg
     2341 atgtgatggc tgctatgggg atggcgcaat cacaagccgg attcggaatg gctgcattct
     2401 gcggtaagca tgaactcagc cagaacgaca aacaaaaggc tatcaactat ctgatgcaat
     2461 ttgcacacaa ggtatcgggg aaataccgtg gtgtggcaaa gctcgaagga aatactaagg
     2521 caaaggtact gcaagtgctc gcaacattcg cttatgcgga ttattgccgt agtgccgcta
     2581 cgccgggcgc aagatgcaga gattgccacg gtacaggccg tgcggttgat atagccaaaa
     2641 cagagcagtg ggggatagtt gctgagaaag agtgcggaag atgtaaaggc gtcggttatt
     2701 caagaatgcc agcaagcgcc gcatatcgcg ctgtgacgat gctaatccca aaccttaccc
     2761 aacccacctg gtcacgcact gttaagccgc tgtatgacgc tctggttgtg caatgccaca
     2821 aggaagagtc aatcgcagac aacattttga atgcgatcac acgttagcgc catgattgcc
     2881 acggatggca acatattaac ggcataatat tgactttttg aataactttg gggaaacttg
     2941 acaccaataa tgggcgtttt ttacatgtca ttgatgagtc tcaataacct gccgccgagt
     3001 agtttttatg ctctgaattg tatttgtgta gtaaacatgc tgactgc
//
LOCUS       DAZFDP010000306         2998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFDP010000306 DAZFDP010000000
VERSION     DAZFDP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..206)
                     /locus_tag="ACHJ1G_005234"
     tRNA            complement(130..206)
                     /locus_tag="ACHJ1G_005234"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Asp,seq:gtc)
     gene            complement(339..1070)
                     /gene="dnaQ"
                     /locus_tag="ACHJ1G_005235"
     CDS             complement(339..1070)
                     /gene="dnaQ"
                     /locus_tag="ACHJ1G_005235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4766894.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1135..1602
                     /gene="rnhA"
                     /locus_tag="ACHJ1G_005236"
     CDS             1135..1602
                     /gene="rnhA"
                     /locus_tag="ACHJ1G_005236"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4766895.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1599..2321)
                     /locus_tag="ACHJ1G_005237"
     CDS             complement(1599..2321)
                     /locus_tag="ACHJ1G_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4766896.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2355..>2998
                     /gene="gloB"
                     /locus_tag="ACHJ1G_005238"
     CDS             2355..>2998
                     /gene="gloB"
                     /locus_tag="ACHJ1G_005238"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4766897.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINV"
BASE COUNT          820 a          722 c          698 g          758 t
ORIGIN      
        1 accatatgtg attaacacat ttacagcata acaaaaccat agaccgaaga aatagcggga
       61 tttaaatgca aaaaaaaccg caaaattaaa aatcttgcgg ttctctgaac tcattttcat
      121 gagtgaattt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
      181 attctaaccg actgaactac cgctccgcgt tgtgttccgt tgggaacgag gcgaatatta
      241 cggattgcct cacccttcgt caacggtttt tctcatcttt tgaatcgttt gctgcaaaaa
      301 tcgcccaagc cgctattttt agcgcctttt acaggtattt atgcccgcca gaggcagctt
      361 ccgcccttct tctccaccag atcaagacgg gcttcatgag ctgcaagctc ttcatctgtc
      421 gcaaaaacaa cgcgtaactt acttgcctga cgtacaatgc gctgaattgt tgcttcacct
      481 tgttgctgct gtgtctctcc ttccatcgca aaagccatcg acgtttgacc accggtcatc
      541 gccagataaa cttccgcaag gatctgtgca tcgagtaatg ccccgtgtag cgttcgttta
      601 ctgttatcta tttcgtagcg ggcacataac gcatcgaggc tgttgcgctt accaggaaac
      661 attttcctcg ccaccgcaag gctatcggtg accttacaga aggtattggt cttcggaata
      721 tcgcgcttaa gcaacgaaaa ctcgtagtcc ataaagccga tatcgaacgc tgcgttatgg
      781 atcaccaact ccgcgccgcg gatatagtcc atgaactcat cggctacttc ggcaaacgtg
      841 ggcttatcga gcaaaaattc atcggcaata ccatgtacgc caaaggcttc cggatccacc
      901 agccgatcgg gtttgagata aacatggaag ttattgcccg tcaggcgacg gttcaccact
      961 tcaacggcac caatctcaat gatcttgtgg ccttcatagt gcgcaccaat ctggttcata
     1021 ccggtggttt cggtatcgag aacgatctgg cgtgtaattg cagtgctcat agcggtcatt
     1081 tatgtcagac ttgtcgtttt acagttcgat tcaattacag gaagtctacc agagatgctt
     1141 aaacaggtag aaattttcac cgatggttcg tgtctgggta atcctggacc tgggggttac
     1201 ggcgctattt tacgctatcg cggacgcgag aaaaccttta gcgcaggcta cacccgcacc
     1261 accaacaacc gtatggagtt gatggccgct attgtcgcgc tggaggcgtt aaaagaacat
     1321 tgcgaagtca ttttgagtac cgacagccag tatgtccgcc aaggtatcac ccagtggatc
     1381 cataactgga aaaaacgtgg ctggaaaaca gcagacaaaa aaccagtaaa aaatgtcgat
     1441 ctctggcaac gtcttgatgc tgcattgggg cagcatcaaa tcaaatggga atgggttaaa
     1501 ggccatgccg gacacccgga aaacgaacgc tgtgatgaac tggcccgtgc tgcggcaatg
     1561 aatcccacac tggaagatac aggctatcaa gtcgaagttt aagcctgtgg tttacgatat
     1621 tgccgggtgg ctccaaccgc ctggcgaatt cgtggcttgt ttttactctg tttcatcgga
     1681 tttagcgtta aaggaatagt ccgtttccgg gcaacaataa gttgtaagca accaagcgca
     1741 ggaatatgtg cattcaatag ttttccaccg tgtttgttcc acgggagaac gtggaaacgg
     1801 ctggcgtgca gcacttcaaa attcaacaaa gagagccagt ccagttgccg catcagagta
     1861 aacatccggc tgttataggg cgaggttttt cgcaataccg gcacaagttt gcgtaatccc
     1921 attaaactaa tgggattgaa gccactaatc accagccagc catcatcaat caatacccga
     1981 tcggtttcac gcaataaacg atgcggatcg gtgcaccacg gcaatgtatg tgccagtaga
     2041 caaacatcaa cggatttatc ggcaaaagga agatgaagtg gatccgcctg cacctggacg
     2101 ggcattcctt gcgcagaaac attcacttga tgagaaaccg cgcacgcttc gcaattgatt
     2161 tctgcgctta aattgccaat cttaagcaga tgaaaaccat acattttagt gaaccacggg
     2221 ttgagctggc gctccagcgc cttgcgataa agctctcccc agggcaaatc gccccagcga
     2281 tcaggagcca cgacagtttg agggactctt gccggtttca tcacaacctt ccgtttcaca
     2341 ctaagaggta atctatgaat cttaacagta ttcccgcctt tgatgacaat tacatctggg
     2401 ttttgaatga tgaagcaggt cgctgcctga ttgtcgatcc cggagacgca gagccagtat
     2461 taaacgccat tgccgccaat aactggcaac cggaggccat atttctcacc caccatcatc
     2521 acgatcacgt tggcggcgta aaagaactgg tggaaaagtt tccgcaaatt gtggtgtatg
     2581 gtccacaaga gacacaagat aagggaacaa cacaggtagt caaagatggc gaaactgcct
     2641 tcgttttggg gcatgaattt agtgtaattg ccacaccggg tcacacttta ggacatatct
     2701 gttacttcag taaaccttat ctattttgcg gcgacacgct gttttctggt gggtgtggta
     2761 ggttgtttga agggacagca tcacaaatgt atcaatcact taataagtta agtgcgttac
     2821 ctgacgatac attggtatgt tgtgctcatg aatatacgtt atcaaatatg aagtttgctt
     2881 tgagtattct tccgcacgat ttgtccataa atgattatta tcgtaaagtt aaggagttac
     2941 gggcaaaaaa tcaaataaca ctacccgtaa ttctgaaaaa tgagcggcaa attaatgt
//
LOCUS       DAZFDP010000307         2996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFDP010000307 DAZFDP010000000
VERSION     DAZFDP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2643
                     /locus_tag="ACHJ1G_005239"
     CDS             <1..2643
                     /locus_tag="ACHJ1G_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766898.1"
                     /translation="DAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTR
                     LERLNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALA
                     RVTRARERYSRVQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALT
                     RIDKNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMA
                     TAGGMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGP
                     VAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSV
                     SDAGGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLP
                     KLRKELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLT
                     RVVSKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLL
                     TGSMNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRA
                     FFAGVFSGIMERLTPLRETFERFSPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKC
                     TSAGEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAI
                     LQDKVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGK
                     LVEETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPAL
                     VTVVAASSRPVAEAIRSPVASVPVTSRIREPVASEFGGEIHVHLHNVVTQNPRELAKL
                     VGEMVRAEMERRARAGRGSFYDKD"
     gene            2656..>2996
                     /locus_tag="ACHJ1G_005240"
     CDS             2656..>2996
                     /locus_tag="ACHJ1G_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766899.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKK"
BASE COUNT          673 a          754 c          924 g          645 t
ORIGIN      
        1 gacgctgtca gtaaagcgga tgcgggtatc gtgaaagcac gacgccagct taacgccctt
       61 aatcagttac aacgcacggg tacagtgctc agcgaaaaac aacaaaagct gatgcagcag
      121 ttaagcaccc ggcttgaacg cctgaatgaa tcgcgcacac gggaaattca gaaaatgcgg
      181 gagcttggcg gagagctgaa acgccacggc atttccctga caggcagcga taacaccatc
      241 cagcaggcca tcagacgcac cgaacagtac aacaaccagc ttgaacgcga acggcaggcg
      301 cttgcgcgtg taacgcgggc gcgtgagcgg tattcgcgcg tgcaggaaac cgcgggaaaa
      361 ctgaaaacag gtggtgcgct ggcaattggt gcggcagcgg ctggcggcta tgctgccggg
      421 cgttttttac agcctgcgat cgggttcggc aaagagatgt cccgcgttca ggcactgacg
      481 cgaatcgaca aaaacagccc gcagtttaag gctctgcgtg agcaggcgtt aaaacttggc
      541 tctgaaacgc agttcaccgc aggcgatgcc gccagtgggc aggcatttct tgcaatggct
      601 ggcttcactc cgcaggccat tcaggctgcg cttccgggcg tgctgagcat ggcaacggct
      661 ggcggcatgg atctcggcga gacggcggat attggctcaa atatcctgac gcagttcggc
      721 ctttctgctg accagatgga ccgggttggc gacacgctca ccgcagcatt tacccgtacc
      781 aacactgacc ttcgcgcact gggcgaaacc atgaaatatg caggtccggt ggcgggcaag
      841 ctgggaatat cgctggagca ggccgcagcg atggcgggcg tgctggcgaa tatgggtatc
      901 agagggagtg atgccgggac ggcaatgcgt gccagcctgg ctcgtctggc atcaccgcca
      961 aaggcggcag cagaggcgct gaaagagctt ggcgtgtctg tctcggatgc cgggggcaaa
     1021 atgcgtccga tggaggatgt gctggctgac ctttataaag ctacccgcaa atacggggaa
     1081 gttgaccggg tatcgttctt taaggacatt gccggagaag aggctttcac atcatttatg
     1141 gccctcgttg atgcggcagg tgacggctcc ttacccaaac tgagaaaaga acttgaaggt
     1201 gcgcgcggtg aggctgaacg cacggcaaag gttatggcca acaaccttga cggcgatctg
     1261 aaatcactca gcagtgcatg ggaagggttg cgcatccgca ttgcagatct gattgacggt
     1321 ccgctgcgtt ctgtcacgca gtggctcacg cgtgtggtat caaaggtgac ggcgctggcg
     1381 caggcccatc ccgcactgac gcgccagcta ctgattgcag gcggtgcact gctggcaatg
     1441 actgcaacgg ttggctcgtt gtcgctggct attggtgtgc ttgctggccc gctggcaaaa
     1501 ctgcgtcttg gtttttccct cctgaccgga tcaatgaatg ctgtcagggt tctgccagca
     1561 ctatggggaa tggtgacggg ttccgtttct ttgctgggag gcgctatcgg ggcgttgttc
     1621 agtccggttg gtcttatcgt ggctgcgctt gccggagctg ccgttcttat ctggaaatac
     1681 tgggatccca tcagggcatt ttttgccggg gtgttcagcg ggattatgga aaggctgacc
     1741 ccgttgcgcg aaacctttga acggtttagt cctgtttttg acgcaatcgg gagtgggatc
     1801 agccaggtgt ttaactggtt taaatcgctg ctgtcaccga tggagtccag caaggaaact
     1861 ctggataaat gtaccagtgc tggcgagata ttcggtaacg ttcttggcgg tgcgttacaa
     1921 cttgttctga cacctgcaaa aatgttgctg gatacgctgg cgtggatact tgaaaagctt
     1981 ggtgtgcttc cggatgaagc ggaaagggcg cgcaagaaaa tcgaagacgc acagcgtgcg
     2041 gccattcttc aggacaaggt tgccttgctt cagggggacc tggcgaaaat caatccgccg
     2101 aagcctgtgg aaaatggcaa tggcaccgga ggtgataacc ccaaagacaa taaaccgctc
     2161 acagacagca ataccggtac gctgcgcaga ctcagcaaaa ttgctgataa cacaggtaag
     2221 ctggttgagg agacgaaaaa acgcattggc cccggcgata ttgtctttaa aaacctgccc
     2281 cgggcacttg ctgttcgtgg ggagtggcag gagcggaaga ttgcgcaggt cagtaagcct
     2341 gcccccgcaa ttaatatcac acccgtggtc ccggctccgc tgcctccggc gctggtcact
     2401 gttgttgcgg ccagctcccg cccggtggcg gaggctatac gatctccagt ggcatcagtt
     2461 cctgtaactt cccgtatccg ggagcctgtt gcctccgaat ttggtggtga aattcatgtt
     2521 catctgcata acgttgttac gcagaatccc cgcgaactgg cgaaactggt cggtgaaatg
     2581 gtcagggcag aaatggaacg gcgcgcccgt gccgggcgtg gcagttttta cgataaagat
     2641 tgaggagtca tggccatgat gatgatctac ggcatgtttg tttttgagct gcgcacgctg
     2701 ccgcatcagc agttacagca aaacaaaagc tggcggcatg tgaaaaatga acgcgttaac
     2761 cgttcagcaa gctggcagta tatcggtgca ggtgatgatc gcatcgttct ttctggtgtg
     2821 ctttatcctg aaattacagg tggcgaagtg tcgctgtcgc tgctgaccac gcaggcgtat
     2881 acaggacgac cctggccttt gattgatggc gtcgggcaga tttacggcat gtatgtcctg
     2941 accggaacga atacgacccg ttccgagttt gatcgctacg gtaaggcgaa aaagat
//
LOCUS       DAZFDP010000308         2996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFDP010000308 DAZFDP010000000
VERSION     DAZFDP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2643
                     /locus_tag="ACHJ1G_005241"
     CDS             <1..2643
                     /locus_tag="ACHJ1G_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4766900.1"
                     /translation="DAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTR
                     LERLNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALA
                     RVTRARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALT
                     RIDQNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMA
                     TAGGMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGP
                     VAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSV
                     SDAGGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLP
                     KLRKELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLT
                     RVVSKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLL
                     TGSMNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAILIWKYWDPIRA
                     FFAGVFSGIMERLAPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKC
                     TSAGEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAI
                     LQDKVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGK
                     LVDETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPAL
                     VPVVAASSRPVAEAIRSPVASVPATSRNRETVASGFGGEIHVHLHNVVTQNPRELAKL
                     VGEMVRAEMERRARAGRGSFYDKD"
     gene            2656..>2996
                     /locus_tag="ACHJ1G_005242"
     CDS             2656..>2996
                     /locus_tag="ACHJ1G_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766901.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKK"
BASE COUNT          676 a          759 c          925 g          636 t
ORIGIN      
        1 gacgctgtca gtaaagcgga tgctggcatc gtgaaagcac gacgccagct taacgccctt
       61 aatcagttac aacgcacggg tacagtgctc agcgaaaaac aacaaaagct gatgcagcag
      121 ttaagcaccc ggcttgaacg cctgaatgaa tcgcgcacgc gggaaattca gaaaatgcgg
      181 gagcttggcg gagagctgaa acgccacggc atttccctga caggcagcga taacaccatc
      241 cagcaggcca tcagacgcac cgaacagtac aacaaccagc ttgaacgcga acggcaggcg
      301 cttgcgcgtg taacgcgggc gcgtgagcgg tattcgcgcg cgcaggaaac cgcgggaaaa
      361 ctgaaaacag gtggtgcgct ggcaattggt gcggcagcgg cgggcggcta tgctgccggg
      421 cgttttttgc agcctgcgat cgggttcggc aaagagatgt cccgcgttca ggcactgacg
      481 cgaatcgacc agaacagccc gcagtttaag gcgctgcgtg agcaggcgtt aaaacttggc
      541 tctgaaacgc agttcaccgc aggcgatgcc gccagtggac aggcatttct tgcaatggct
      601 ggcttcacac cacaggccat tcaggctgcg ctccctggcg tactgagcat ggcaacggct
      661 ggcggtatgg acctcggtga gacggcggat attggctcaa atattctgac gcagttcggt
      721 ctttctgctg accagatgga ccgggtcggc gacacactca ccgcagcgtt tacccgtacc
      781 aacactgacc ttcgcgcact gggtgaaacc atgaaatatg caggtccggt ggcgggtaag
      841 ctgggaatat cgctggagca ggccgcagcg atggcgggcg tgctggcgaa tatgggtatc
      901 agggggagtg atgctggtac ggcaatgcgt gccagcctgg ctcgtctggc atcaccgcca
      961 aaggcggcag cagaggcgct gaaagagctt ggcgtgtctg tctcggatgc cgggggcaaa
     1021 atgcgcccga tggaggatgt gctggctgac ctttataaag ctacccgcaa atacggggaa
     1081 gttgaccggg tatcgttctt taaggacatt gccggagaag aggctttcac atcatttatg
     1141 gccctcgttg atgcggcagg tgacggctcc ttacccaaac tgagaaaaga acttgaaggc
     1201 gcgcgcggtg aggctgaacg cacggcaaag gttatggcca acaaccttga cggcgatctg
     1261 aaatcactca gcagtgcatg ggaagggttg cgcatccgca ttgcagatct gattgacggt
     1321 ccgctgcgtt ctgtcacgca gtggctcacg cgtgtggtat caaaggtgac ggcgctggcg
     1381 caggcccatc ccgcactgac gcgccagcta ctgattgcag gcggtgcact gctggcaatg
     1441 actgcaacgg ttggctcgtt gtcgctggct attggtgtgc ttgcaggccc gctggcaaaa
     1501 ctgcgtcttg gtttttccct cctgaccgga tcaatgaatg ctgtcagggt tctgccagca
     1561 ctatggggaa tggtgacggg ttccgtttcg ttactggggg gcgctatcgg ggcgctgttc
     1621 agtccggttg gtcttatcgt ggctgcgctt gccggagctg ccattcttat ctggaaatac
     1681 tgggacccaa tcagggcatt ttttgccggg gtgttcagtg ggattatgga gcggctggcc
     1741 ccgttacgcg aaacctttga acggtttggt cctgtttttg acgcaatcgg aagcgggata
     1801 agccaggtgt ttaactggtt taaatcgctg ctgtcaccga tggagtccag caaggaaacg
     1861 ctggataaat gtaccagtgc tggcgagata ttcggtaacg ttcttggcgg tgcgttacaa
     1921 cttgttctga cacctgcaaa aatgttgctg gatacgctgg cgtggatact tgaaaagctc
     1981 ggtgtgcttc cggatgaagc ggaaagggcg cgcaagaaaa tcgaagacgc acagcgtgcg
     2041 gccattcttc aggacaaggt tgccttgctt cagggggacc ttgcgaaaat caatccgccg
     2101 aagcctgtgg aaaatggcaa tggcaccgga ggtgataaac ccaaagacaa caaaccgctc
     2161 acagacagca ataccgggac gctacgcaga ctcagcaaaa ttgctgataa cacaggtaag
     2221 ctggttgatg agacgaaaaa acgcattggc cccggcgata ttgtctttaa gaacctgccc
     2281 cgcgcacttg ctgttcgtgg ggagtggcag gagcggaaga ttgcgcaggt cagtaagcct
     2341 gcccccgcaa ttaatatcac acctgtggtt ccggctccgc tgcctccggc gctggtccct
     2401 gttgttgcgg ccagctcccg cccggtggca gaggccatac gatcgccagt ggcatcagtt
     2461 cctgcaactt cccgtaaccg ggagactgtt gcctccggat ttggcggtga aattcatgtt
     2521 catctgcata acgttgttac gcagaatccc cgcgaactgg cgaaactggt cggtgaaatg
     2581 gtcagggcag aaatggaacg gcgcgcccgt gccgggcgtg gcagttttta cgataaagat
     2641 tgaggagtca tggccatgat gatgatctat ggcatgtttg tttttgagct gcgcacgctg
     2701 ccgcatcagc agttacagca aaacaaaagc tggcggcatg tgaaaaatga acgcgttaac
     2761 cgttcagcaa gctggcagta tatcggcgca ggtgatgatc gcatcgttct ttctggtgtg
     2821 ctttatcctg aaattacagg tggcgaagtg tcgctgtcgc tgctgaccac gcaggcgtat
     2881 acaggacgac cctggccttt gattgatggc gtcgggcaga tttacggcat gtatgtcctg
     2941 accggaacga atacgacccg ttccgagttt gatcgctacg gtaaggcgaa aaagat
//
LOCUS       DAZFDP010000309         2963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFDP010000309 DAZFDP010000000
VERSION     DAZFDP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..1356
                     /locus_tag="ACHJ1G_005243"
                     /pseudo
     CDS             125..1356
                     /locus_tag="ACHJ1G_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1341..1979
                     /locus_tag="ACHJ1G_005244"
     CDS             1341..1979
                     /locus_tag="ACHJ1G_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO4766902.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2058..2327
                     /locus_tag="ACHJ1G_005245"
     CDS             2058..2327
                     /locus_tag="ACHJ1G_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4766903.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2348..>2963
                     /locus_tag="ACHJ1G_005246"
     CDS             2348..>2963
                     /locus_tag="ACHJ1G_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766904.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSE"
BASE COUNT          774 a          627 c          818 g          744 t
ORIGIN      
        1 caaatccaac aaccagatta atatctgaag agttttattg ttattattta tccccaaagc
       61 ggtgagttat taatgaagtt tgtctgaatg cataaacaga attattcgac ataaagccag
      121 gaaaatgagt gatacgcttt taaccgaaaa aatccttacc ggagaaaatg ttttgcgtgc
      181 cgcaatcgcc cgcattgagt ggatttttga aacatttccg tcggtatgcc tgtctttttc
      241 aggtggaaag gattccactg tgcttttcca tcttgtggca gaggtggccc gcaggagaaa
      301 acgtcatttc tctgttctgt tcattgactg ggaggcccag tatcggtgca ctattgaaca
      361 tattcagaag atgcgggaaa tgtaccatga tgtgacggaa accttttact gggtggcact
      421 tccctgacca cggtaaacgg cgtctcgcag tttcagccgg agtggatatg ctgggagcca
      481 ggagtgacct gggttcgcca gccaccggaa gaggccatta cagacatgac gtattttccc
      541 ttttaccggt atgccatgac gtttgaagaa tttgttccgg cattttcttc ctggtttgcc
      601 ggtaaccggt gtggagtggc aatactgact ggtgtccgtg ctgatgaatc gctcaatcgc
      661 tttatgggac tggtatctca gcgcaaactg agatatgccg atgataagcc ctggaccacg
      721 gcatcgcctg aagggtttta ttacaccatg tatccgttgt atgactggaa ggcccgcgat
      781 atctggatat ataacgccag aacctgtgcc atttacaatc ctctgtatga cctgatgtac
      841 cgtgccggcg tgccgttacg caacatgcgt gtctgtgaac cttttggtcc tgaacagcgt
      901 aagggactgt ggctttacca tgttctggag ccggaaacct gggccaggat gtgtgaacgg
      961 gtgtcagggg ctgccagcgg ggcgctttat gccaatgaga gtggtgccta ttttgccctg
     1021 tgtaagcgta tcacgaagcc gcctcatcat acctggcgca gctatgcgat gttcctgctg
     1081 gatgtgatgc cggaaagaac agcagaacat taccgtaata aaattgctgt ctacctgcgt
     1141 tggtatcaga cgcggggctt cccggatgac attccggatg aacaggagaa tgacctgggg
     1201 agccgggata ttccgtcctg gcgacgtatc tgtaagactc tcataaagaa tgatttctgg
     1261 tgccggaccc tctcctttag ccctaacaaa ccccggcact atgaacgtta cctgcagcgt
     1321 atgaaggaaa ggaggaagga atgggggatt ctgtgacacc ggagatactt ggcaacatga
     1381 ttcgtcagta cttcagccag gaacgaccag aagaagaggc catacaggcc cttaatcacc
     1441 tgcgcagggt gcttcatgag gtcagtcctt ttgcacagga gcctgtggac tgtgtgctgt
     1501 gggtaaaagc agatgaggtg gtggccaatg attacaatcc taatgtcatg gcgccgggtg
     1561 agaagaaact gctgaagcag tcactggaga aagatggttt cacacaaccg gtggtagtgt
     1621 ctgaggaaaa gaaccattac ttggtggtgg atggttttca tcgccaggta ctgggcaggg
     1681 aagcaggtac gggaaaacgt ctgaagggct ggctgccggt ggtctgtatt aatccggaga
     1741 gaaagggaca ggcctcacgt attgctgcga ccatccgaca taaccgggcc aggggcaaac
     1801 atcagataac ctcaatgtct gatattgtca gggacctgtc gcgcctgggc tggacgaatg
     1861 aacgcattgg tacggagctg ggtatggacc aggatgaagt tctcagattg aaacagatta
     1921 gcgggctgac ggaactgttt caggaagagg atttcagccc ggcatggacg gttcgctgag
     1981 accgtcagat atgtatatta tttcagtgag ttaatgtttt gagcaggaaa aagagtaccc
     2041 cggaagttgt ggaagacatg gttgcgcaga aactggaggc tgctggttgc tggcgcagag
     2101 cttctgaccg ctggcttttt gttatgggaa atgttgaatg tacagaagca caacgggagt
     2161 ggttgttgtt gcgccggaat tattgtctgg cgcagatatc gtccccgccg ctacctgaaa
     2221 cgctggatat cagcgaggtg gcgaaagctg cagatgccac gctgaggcga atggggattg
     2281 ccactccgtc cggggaggta ttcagaaaag gtacaccggt ctgttgatat tcaggtaaca
     2341 ggtattaatg atgaagcaag gaatggcaaa cagtatatca atgacatggc aacttttatg
     2401 cgatgaacat ggttttaccc gggagcacta caatcagctg aagaagttca gcccggagac
     2461 attacgtgaa attattgctg aaatagccag ctgtcatcca tcaaccagtg tattgctcag
     2521 gaataagtgg ctgacaccgc cggaggatat tctggagcag attacccgtg aatacgaacg
     2581 aagaatacaa aactgtccac cgttcaggag cgagaaggaa gcggaatcct ggttaaatga
     2641 actatctttt gctgtgattg ctccgctata ccgtgttgcc cccgaacaaa cagaacaggt
     2701 tgagtccttt gttctgcagc ttattgccga gcaggagcgg gtttgggagc ttattttaac
     2761 caatgatggt tattcatggc attgcgcact ttatgatata ttatttcatc tggttttacg
     2821 aaatatggaa gaccagcctg aacatcagaa acagcgaata acaaaaatat tttatgagcc
     2881 cgatgcaaga ctggttgcag aagagattaa attccggatt caatctctgt atgatgatga
     2941 acaaaagaga gctttaagtg aac
//
LOCUS       DAZFDP010000310         2956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFDP010000310 DAZFDP010000000
VERSION     DAZFDP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..1288
                     /gene="bluF"
                     /locus_tag="ACHJ1G_005247"
     CDS             77..1288
                     /gene="bluF"
                     /locus_tag="ACHJ1G_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO4766905.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1493..2224
                     /gene="bluR"
                     /locus_tag="ACHJ1G_005248"
     CDS             1493..2224
                     /gene="bluR"
                     /locus_tag="ACHJ1G_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO4766906.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2445..2849
                     /gene="ycgX"
                     /locus_tag="ACHJ1G_005249"
     CDS             2445..2849
                     /gene="ycgX"
                     /locus_tag="ACHJ1G_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO4766907.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          872 a          624 c          639 g          821 t
ORIGIN      
        1 ggtattaaaa gaaagacaca agaagatatg tctgttaccg tcttactctc gcctcaccca
       61 ttaccctgga ttgttaatgc ttaccaccct tatttatcgt agccatatac gtgacgacga
      121 acctgtcaaa aaaatcgaag aaatggtttc gatagcaaat cgcaggaaca tgcagtctga
      181 cgtaacaggg atcttactgt ttaatggttc tcattttttc cagcttctgg aaggtccgga
      241 agaacaggtt aaaatgatat atcgggctat atgccaggat ccacggcact ataatattgt
      301 tgagctgctg tgcgattacg cgcctgctcg ccgttttggc aaagcgggaa tggaattatt
      361 tgatttgcgc ctgcacgagc gagatgacgt tttacaggcc gtattcgaca aaggcacatc
      421 aaaatttcag ctaacttatg atgacagagc gctacaattt tttcgtactt ttgtccttgc
      481 aaccgaacaa tcaacctatt tcgagatccc tgccgaagac tcctggcttt ttatcgctga
      541 cggatctgat aaagaacttg attcctgtgc cctttcacca actataaacg accactttgc
      601 ctttcatcct attgtcgatc ccttatcgcg gcggataatc gcttttgaag ccattgtgca
      661 aaaaaatgaa gatagcccat cagccatagc ggttgggcag cgtaaagacg gggaaatcta
      721 cacagcggat ctcaaaagta aggcgcttgc attcacgatg gcacacgcac ttgagctcgg
      781 tgataaaatg atttcaatca atctattacc tatgaccctg gttaacgaac ctgacgcagt
      841 ctctttttta cttaatgaaa taaaggccaa tgctctggtg cctgaacaaa tcatcgttga
      901 atttactgaa agtgaagtca tatctcggtt tgatgagttt gccgaagcga ttaaatcgct
      961 aaaggctgct ggtatcagtg tagcaattga tcattttggc gcaggttttg ctggtttgtt
     1021 actcctgtca cgcttccagc ctgacagaat taaaatcagt caggaattga ttaccaatgt
     1081 tcataaaagt gggccacggc aggcaattat tcaggcgatc ataaaatgct gtacatcact
     1141 tgaaattcaa gtcagtgcta tgggcgtggc aacaccagaa gagtggatgt ggcttgaatc
     1201 cgcaggaatt gagatgtttc agggagatct gtttgctaaa gctaaattga atggtatccc
     1261 ttccatagcg tggccagaga aaaaataatt ttcagcacat tctttcacat gatttcagta
     1321 aatcatcgtg aaaatgaata cgttacgttt aaatttaatc cacattaaaa ataatgtttc
     1381 atccaattta gctgaaatct tatttatact ttgtacaatt ttagcgtata ttgtacaaag
     1441 tattattggg tcgtgtacag gcgacggaga tttgtgaccg caaggaggaa ttgtggctta
     1501 ttacagcatt ggtgatgttg ctgaacgttg cgggattaat cctgtcactc tccgggcctg
     1561 gcaacgccgc tacggtttgt taaaaccaca gcgcagtgaa ggcggacacc gactctttga
     1621 tgaagaagac atacaacgca tcgaagagat caagcgttgg ataagtaatg gcgtccctgt
     1681 aggcaaagtt aaagcattac tggaaaccac cagccaggat acggaagatg actggagccg
     1741 cctgcaagaa gagatgatgt caattcttcg catggctaat cctgccaaac tacgcgcgag
     1801 aattatttca ctgggtcgag agtacccagt agatcaattg attaatcatg tttaccttcc
     1861 tgttcgccag cgtctcgtgc ttgatcacaa cacctcccgc attatgagca gtatgtttga
     1921 cggcgcatta attgaatacg cagcaacctc gcttttcgaa atgcgccgta agcccggtaa
     1981 agaagccatt ctgatggcgt ggaatgttga agagagagca cgattgtggc tggaagcatg
     2041 gcgtttatca ttgtcaggat ggcacatttc tgttcttgct gatcccattg aatcgccgcg
     2101 cccggaactg ttcccgacgc aaacattgat tgtctggaca ggcatggcac caacgagaag
     2161 gcaaaacgaa cttttacaac attggggtga gcaaggttat aaagtcatct ttcatgcccc
     2221 ctaatttcac ctttatcccg ccccttagac agaatgttat aatctgaacc cacaacagcg
     2281 tttgtaccgg agctggtccg ttgcaaaatg ctcaccggga gaattcagat attccctcta
     2341 tcacactctg tacatatcac agccaaagga cagaccagat tgaagtggta aaaccgatcg
     2401 tgcacactac gcagtctcac aactaacaag aggttattat cctgatggat caggtcgttg
     2461 tttttcaaaa gatgtttgag caagtgagaa aagagcaaaa cttctcctgg ttttattcag
     2521 aattaaaaca tcaccgtatt gcacattaca tttattatct ggctacggat aacatcagaa
     2581 ttattactca cgatgacacg gttttgttat taagaggaac caggaacctg ttaaaagtta
     2641 gtacgaccaa gaaccctgct aaaataaaag aggccgcatt gcttcatatt tgcggaaaat
     2701 ctacatttcg ggaatactgt tcaacactag caggcgcggg cgttttccgg tgggttactg
     2761 atgttaatca taacaaacgc agttactacg ccattgataa tacgctttta tatattgaag
     2821 atgtagaaaa taataaacca ttaatctagc taaagttgga tgcttaagaa atgcttcata
     2881 attcagtaag gcattagcat aatgggaata aaagtacaga gactatccct atggatgata
     2941 aatactgtcc ttttat
//
LOCUS       DAZFDP010000311         2948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFDP010000311 DAZFDP010000000
VERSION     DAZFDP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..510
                     /locus_tag="ACHJ1G_005250"
     CDS             <1..510
                     /locus_tag="ACHJ1G_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO4766908.1"
                     /translation="IESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVI
                     NDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAI
                     IGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIIT
                     SAIDKLKRS"
     gene            538..1496
                     /locus_tag="ACHJ1G_005251"
                     /pseudo
     CDS             538..1496
                     /locus_tag="ACHJ1G_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1508..1969
                     /gene="yibG"
                     /locus_tag="ACHJ1G_005252"
     CDS             1508..1969
                     /gene="yibG"
                     /locus_tag="ACHJ1G_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4766909.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2604..>2948
                     /locus_tag="ACHJ1G_005253"
     CDS             2604..>2948
                     /locus_tag="ACHJ1G_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO4766910.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQ"
BASE COUNT          949 a          525 c          655 g          819 t
ORIGIN      
        1 atcgagtcaa cggctcagcg atgcaagaaa aacccaattt attcacctaa aatagtagaa
       61 caatctcaaa ttactgcttt tgataaatcg actaatgtca gacgtgctac agcatttgct
      121 atttctgtta tcaatgataa agcaacaatt ccactattga ttaatctgtt aaaagatcca
      181 aatggagatg tcagaaactg ggccgcattt gcaataaata tcaataaata tgataatagt
      241 gatattaggg attgttttgt ggagatgctt caggataaaa atgaagaagt ccgtattgaa
      301 gcaataatcg gactttccta cagaaaagat aaaagggttt tatctgtttt atgcgatgag
      361 ttaaaaaaaa atactgttta tgatgatatc attgaagctg cgggtgaatt aggtgataaa
      421 acgctacttc ctgttttaga tactatgttg tacaagtttg atgacaatga aattataact
      481 tccgctattg ataagctgaa gcgttcatag cgtgagttgc ctatgcacag tgggggattc
      541 ccgccggcac ggtgcagatg ctcgaccggc tggaaagtga aatcctggct gaccgggtga
      601 gtgaggaaag ccgccgctgg ctggcatcgt gcggcctgac cgtggagcag atgaaaaacc
      661 agatggaccc ggtgtacacg ccggcgcgaa aaatccacct gtaccactgc gaccatcgcg
      721 gcctgccgct ggcgctcatc agcacggaag gggcaacagc gtggtgcgca gaatatgatg
      781 aatggggcaa cctgctgagt gatgagaacc cgcatcatct gcagcagctc attcgcctgc
      841 cgggtcagca gtatgatgag gagtccggcc tgtattacaa ccgccaccgc tattatgacc
      901 cgctgcaggg gcggtatatc actcaggatc cgattgggct gaagggggga tggaattttt
      961 atcagtatcc gttgaatccg gtcataaatg tagatccgca aggtttggtt gatataaatt
     1021 tataccccga aagtgatctt atccattctg tagctgatga gattaatatc ccaggcgttt
     1081 tcacaatcgg ggggcatggt acccccacat ctattgaatc cgcaacgcgc agtatcatga
     1141 cagctaaaga tctagcatat ctaattaaat ttgatgggaa ttataaagat gggatgacag
     1201 tttggttatt ttcttgtaat acaggtaaag gacaaaattc atttgctagc caattagcta
     1261 aagagttaca tacaaatgta ataggacctg acacgctatg gacgtggtgg gggcgaggaa
     1321 ctaatggtaa gttaaaaatg gatacagtgc taacagcacc aacgaacctt aattcaaata
     1381 aggatctaat ggctataaca acaaaagacc ttggtaattg gataacatat gggccatctg
     1441 ggcaccccat ttctaatatg caaggtacgc cagaaaaacc cagtgatata agataggttg
     1501 tagatgtatg aaagcatgct tgttactatt tttttatttt tctcttattg gtcaattgca
     1561 tggtgctgat gtgaaaataa aagaaaacga aagtgtgatg ggatctacag caatgaccta
     1621 tgatttaagt gaagaaaagc tgatgaaact gaagtataaa tcacaacatg gtgattcaga
     1681 ggcatcattt cgactatatc aatattactg ctttactaaa aataatattg ataaacaatt
     1741 gcgattcttg gaaagatcag catctcaggg gaatgttaca gcgcagttta attatggggt
     1801 ctttttatca gatacaaatc caacattatc agaatattat aatttgaata gagctattta
     1861 ttggatggaa ttcgctgtaa ataacggtaa tattgatgca aagagcaaac ttcaagagct
     1921 caaaaagcta aagcgaatgg atagaaggaa gaataaggag aatccgtaaa agcaatagtt
     1981 catcagactg ccgcagcagt agtatgatga tgaatcaggg ctgtactata actattatcg
     2041 gtaacattaa ccgcagcagg ggcggtataa cactcaggct ccgattgggc tggaaggtag
     2101 gtggaatttt tatcagtatc cgttgaaccc gatttctgga atcgatcccc ttgggattag
     2161 ccacctgttt atacagtata actttaggta tgctttcgtg tgtttcagat ataccatgtg
     2221 atgataattt ttatgatgtg cttagtattc cagtcgcttc tggtaataat atgcaatgta
     2281 aaaataatcc ccgttgtaca catttataaa tagatggcca atacctcaag gcctatggag
     2341 ttagaatgtt aatgacccgg gagcaagcaa tagaaaacct gacggcatac gattagtacc
     2401 gtcagttaat acaggaactt ataatcgcaa tggattttca atacatagtt gtttaaacgc
     2461 ttttggtccg tctttaggac ctcgattctg ttccgagggt tgtataactg gtttatctaa
     2521 tgacatgcaa aaattaaatg agttaatatt ttcagagcca gatagcacat taacagttac
     2581 ggactaaaaa atatggcaag gttattaaca attgtatttt tttctctatt tctgcgagtg
     2641 cagttactgc aagacaattt cagagagagt tagatttggc agacaaagca atattatggg
     2701 cactaatatc agccagtact aaagagggac gaaaagcgtg ttcactaagc tattttgcct
     2761 gtaaggccgc agaggctgaa ctcggactgg catatatggc tgcgaatgat aacaaggaat
     2821 ttctcacatc cttatctaat ataatgaggt acaaaataga tgctggactt tccgaatcct
     2881 atacatgcta tttattaagt aagggaaaga tcataagacc atatctgaaa aatctaaatc
     2941 cactccaa
//
LOCUS       DAZFDP010000312         2942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFDP010000312 DAZFDP010000000
VERSION     DAZFDP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(332..655)
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005254"
     CDS             complement(332..655)
                     /gene="mobA"
                     /locus_tag="ACHJ1G_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO4766911.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            940..1134
                     /locus_tag="ACHJ1G_005255"
     CDS             940..1134
                     /locus_tag="ACHJ1G_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4766912.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            1622..1783
                     /locus_tag="ACHJ1G_005256"
     CDS             1622..1783
                     /locus_tag="ACHJ1G_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4766913.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHVLTPLAAAIGNCHGGVNL
                     EVMHLLRLN"
     gene            complement(2030..2407)
                     /locus_tag="ACHJ1G_005257"
     CDS             complement(2030..2407)
                     /locus_tag="ACHJ1G_005257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766914.1"
                     /translation="MNDFNPISQGGSTSGSSASYNPNPNYSLINNRVEKYSDKIGTGD
                     DAKSSVVWFVITFTLTMYACLISALVIVDIFNNKGVNILNNIKESWAVFTPIITLSLG
                     YMFGRVEASHAANKESVKPINKD"
     gene            complement(2407..2769)
                     /locus_tag="ACHJ1G_005258"
     CDS             complement(2407..2769)
                     /locus_tag="ACHJ1G_005258"
                     /inference="COORDINATES: protein motif:HMM:NF045442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6864 domain-containing function"
                     /protein_id="HFO4766915.1"
                     /translation="MKIEAEVDGKELVYSSSLTMNPNGKLVFNFDNEARITVVFISED
                     TGDNGNRLVKSTVDDDGLGMTISCYNFKMYSPREEGLASEPQFIFTKNNKKHYFNFRT
                     NLFNSESRWISINFLRDK"
BASE COUNT          910 a          727 c          565 g          740 t
ORIGIN      
        1 aaaaaaacca ccgaataatt cacttatccc cccactctcc tttgcctcat caataaaaaa
       61 cagaacaatt atacatcaaa acaaacagtt acatcccctt cactccaata cttttaacat
      121 aatcccttaa ttgtacgcac cgctgaaacg cgctgaacgc aattctgacg caaataccgc
      181 aaaaagagag gtttagcccc gaaaaaacag gaaggatgcg tcaggatcgt tttcagctca
      241 ctgcatagct atgcatgaaa gcgaatggtg atcactttgg gagcttacgg tgttcatacc
      301 gtctgttttc gacagttttc tccccgggaa gctaatctgc cataagcctg gataacaggg
      361 cacggtgata ctccgtaata gcgatcagta cctccgcata ctccctgtcc ccgacacgct
      421 tgccgtcgat aaagagtttt ttctgtaacg cccccagtct cataacttct ttcagtaccc
      481 gatcatcagt cagtgagtta actttcttac cgagagctgc cgcccgtaaa aaaccggaaa
      541 cggtcttgcc gcattcagca gccctttttc tgatttcctg gtcttcttct gccgtcagac
      601 gaacagggcg actgatagcc cgcctgcgcg tattacttcc acttcgtttt gtcataaaac
      661 atggctccgt atctgacatg gggtgtcggg gcaaagccct gaccagggta attgtaatag
      721 cgtgcatgta tgcgcggtat aacaattaca catcctgtcc tgtcagcaag ttcgaattac
      781 cgacaaagac acggcagttt tttggcagtg acagtattgt caggcagaaa tacccgtcaa
      841 agcgtcagac ggcagaatac cggcggtaaa acagtcgaaa agtggtacgc gatataatca
      901 cacaacgcag atgacagaca gaacggagaa aacgaaaaga tgaacaagca gcagcaagcc
      961 gtactcaaca tggcaggatt cataaaaagc cagagcctga cgctgctcga aaaactggac
     1021 gcactcgatg ctgacgaaca ggccgccatg tgtgagaagc tgcacgaact cgcggaagaa
     1081 cttcagaaca gcatacagac acgctttgaa gcggaaaatc gcaccgggat ataacgcatc
     1141 cggacaggat gcagaacggc atagaggagc gtaaggagaa aatgtcacat aaggcgctct
     1201 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggtgtca
     1261 gctcactcaa aagcggtaat acggttatcc acagaatcag gggataaagc cggaaagaac
     1321 atgtgagcaa aaagcaaagc accggaagaa gccagcgccg caggcgtttt tccataggct
     1381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagc cagaggtggc gaaacccgac
     1441 aggactataa agataccagg cgtttccccc cggaagctcc ctcgtgcgct ctcctgttcc
     1501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1561 tcatagctca cgctgttggt atctcggccc ggtgtaggtc gttcgctcca agctgggctg
     1621 tgtgcacgaa ccccccgttc agcccgactg ctgcgcctta tccggtaact atcgtcttga
     1681 atccaacccg gtaagacacg tcttaacgcc actggcagca gccattggta actgtcatgg
     1741 aggagtaaat cttgaagtca tgcacctgtt aaggctaaac tgaaaaaaca agttttggtg
     1801 actgagctcc tccaagccag ttacctcggt taaaagagcg ccaactcttt taaccttcga
     1861 aaaaccgcct gccaaggcgg ttttttcgtt tacagggaat gagattacgc gcagaaaaaa
     1921 aggatctcaa gaggatcctt gcatgtctta cctcccccta acgataaagc aagccatcaa
     1981 gttcatgaat gaacatattc ctatccttct ctcaaaaaat aagaaataat taatccttgt
     2041 tgatgggttt tacactttct ttgttggccg catgcgatgc ctcaacccta ccaaacatat
     2101 aacctaggga taatgttatt attggcgtaa aaacagccca tgattctttt atattattaa
     2161 gaatattgac acctttattg ttaaagatat caacaataac aagtgcagat attaaacatg
     2221 catacatagt caatgtaaat gttatgacaa accaaacaac actacttttt gcatcatcac
     2281 cagtccctat cttatctgag tatttctcca cccgattatt tattaaagaa taatttggat
     2341 ttggattata agaggccgaa ctaccagagg tagatccacc ttgagaaatt ggattaaagt
     2401 cattcattac ttatccctca agaagttaat actaatccat ctcgactcag aattaaaaag
     2461 atttgttcta aaattaaaat aatgcttttt attatttttt gtaaagataa actgaggctc
     2521 tgaagccaat ccttcttctc ttggactata cattttgaaa ttataacaag agattgtcat
     2581 ccccaatcca tcatcatcga ctgtagattt aacaagccta ttgccattat cacctgtatc
     2641 ttcagaaata aaaacaactg taattcttgc ttcattatca aaattaaaaa ccaattttcc
     2701 attaggattc atagtcagag aactagaata taccagctct tttccatcaa cttcagcttc
     2761 aattttcata ttatctcatc ttataaacaa taataaccaa cacagaaaaa accaaaacaa
     2821 taaacccagg cattacaaca acccgatgaa gtttatgtaa aaacattgac ttaattagac
     2881 catgcataac atgctttaaa agcagaaata agatcacaag aagtataatt attatcaatg
     2941 tt
//
LOCUS       DAZFDP010000313         2923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFDP010000313 DAZFDP010000000
VERSION     DAZFDP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..1326
                     /gene="nleA"
                     /locus_tag="ACHJ1G_005259"
                     /pseudo
     CDS             5..1326
                     /gene="nleA"
                     /locus_tag="ACHJ1G_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
     gene            1541..1760
                     /locus_tag="ACHJ1G_005260"
                     /pseudo
     CDS             1541..1760
                     /locus_tag="ACHJ1G_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2020..2667)
                     /locus_tag="ACHJ1G_005261"
     CDS             complement(2020..2667)
                     /locus_tag="ACHJ1G_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4766916.1"
                     /translation="MPINLTAYLTASGGLGEIPQDTLSGIRILAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
BASE COUNT          923 a          651 c          533 g          816 t
ORIGIN      
        1 tattatgaac attcaaccga ccatacaatc tggaatcacc tcacaaaaca atcaacatca
       61 tcaaacagaa caaataccct ctacacaaat accgcaatcc gaattacctc taggatgcca
      121 agctggattt gttgttaata ttccagatga tatacagcaa catgcaccgg aatgcggtga
      181 aacaacagct ctactgagct tgataaaaga taaaggtctg ctctcagggc tagacgaata
      241 tatagctcct caccttgaag aaggatccat aggaaaaaaa cattggatat gtttggttta
      301 ttcaatgtta cccaaatggc attagagata cctagttccg tttcaggcat ctctggtaaa
      361 tatggtgtcc agctaaacat tgtaaaacca gatattcatc ctacatcagg taattatttt
      421 ttacagatat tccctttgca tgatgaaata ggtattaatt ttaaagacct tcctggtcca
      481 ttaaaaaatg cattaagcaa cagcaatata ccaaccactg tatcgactgc tgcatccact
      541 attgcatcag ccactacttc gacggtaacc accgcgtcaa aagacccaat accatggttt
      601 ggattaacag ctcaagtagt tcgtaatcat ggtgtggaac ttcctatagt caaaactgaa
      661 aatggatgga agcttgttgg agaaactcct cttactcctg atggccccaa agcaaattat
      721 actgaagagt gggtgatcag accgggagaa gcagatttta aatatggtgc atctccacta
      781 caggcaactc tagggctgga gtttggcgca catttcaagt gggatttaga taaccctaat
      841 actaaatatg ccgttcttac caatgctgcc gcaaatgcgc ttggtgctgt agggggattt
      901 gcagtatcca gatttactgg tacagatcca atgttaagtc ctcatatcgg tgcaatggtt
      961 gggcaagcag cggggcatgc catacagtat aatacccccg gattaaagcc agacactatt
     1021 ttatggtggg caggtacgac tctggggctg gcagatttaa acaaagccga gacaggtgaa
     1081 ggcagattca ctgactatcc tcgtatatgg tggcacgcaa gagaaggtgc catcttccct
     1141 aataaagaag atattgcccg tgcaacaggg gctgatatac gagctatgga agaaggtata
     1201 cccgttggac agcgacatcc aaatccagag gatgtggtca tcgatatcga aagcaatggc
     1261 tcaccacatc ataacccatc aaattatgtt gatacctttg atataatcca agaaacaaga
     1321 gtctaagctt tacttattta tttaaaaata atagctgaaa tggatgacag ctattattaa
     1381 taaggattaa tatatctttt tcctaaaaat cccacaaaac aaatagcctg taactgtttt
     1441 tcatccataa attaaccttc atttgatgtt gggttgaaca tatcaaaatc aggcaattac
     1501 acaaatctat gtacaggctc gtttagaatt tttctggtgt attgctaacg aactggccaa
     1561 aaacctcagc cccctcagaa gacctcagtc agtttgatcg gttgctgaaa aagctcagcg
     1621 ttgaagccgc tctcaatgca gagatgacac accatcttgg gaatgagaaa aatcagtcca
     1681 gatcaggagc taactcccgc aacggttatt ccacaaagac cgttatcaca ggcgacggtc
     1741 cactggaact gcgtacgccg caccgttctg catacgacgt actgcgattt ttttacaatt
     1801 tatccctgca ctggtgaatt atgggcactc tgaatacata cataacacat gatgtctgtt
     1861 atgctttttt tcacgcacat ttccaattat gctaacagaa atgtccagac aataccctct
     1921 ccttttatcc ctgtatacag tccatcaatt agaaaattaa cagaaaaaac tgaaaagatg
     1981 tctgtgaatt acacttatca gtcaatatgt tctaacatct tatagtctag ttatctcacc
     2041 acgaacattt ttaatacttc ctgatgtgaa atcaaaatag cacgagtttt ccttaacaat
     2101 cattgactct gttatttttt ctctgctcag aggatgaggt gaacttgtgt taacaagatg
     2161 ttttaatgat tctttatcat ataaagtaca tacatctgaa tctccagcat ttctcataat
     2221 cacaccatgc tcagggatgc ataatgtaat cgggcatgtt aatgcctctt cagtcgccct
     2281 cagagatgac gagttaacat taaatgagca tttttctatt tgctctaaca ccaactctgt
     2341 ttgcattaca gaaggacgag gtggagaagc atacgggctg ggtatacttg ctaaaatctg
     2401 gcggccggat agccctccat taaggtcgcg ttcaagacga ttaacattat tcaatgcagt
     2461 tcgtaaagct ctctctgata atccttcagg gcttacactt cccataaaga agcctaaaaa
     2521 gacagaacgt attgtaacaa tagtgctccc catctgaacc tgagctcctc cgttaaaagc
     2581 cagtattcgt atgccagaaa gagtatcctg cggtatttcc cccagtcccc cactcgctgt
     2641 tagatatgct gttaaattta ttggcataat aaaaccccca ttaacttcat taacatttta
     2701 cttgatttcc tgttgaagca tcttacttat cacaatgctt tcacacacca ttatcagtct
     2761 tgcaaaagat aagactccat tatcaagcca aaataaattt aacttcatta acaaacatca
     2821 ccatgacatg acaacaaaaa accggagcca gactccggtt ttttgaagct gtcgggttac
     2881 ttcatccagc caatattttc ccacgtcccg tcagcacgca gaa
//
LOCUS       DAZFDP010000314         2920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFDP010000314 DAZFDP010000000
VERSION     DAZFDP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(208..810)
                     /locus_tag="ACHJ1G_005262"
     CDS             complement(208..810)
                     /locus_tag="ACHJ1G_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766917.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(897..1103)
                     /locus_tag="ACHJ1G_005263"
     CDS             complement(897..1103)
                     /locus_tag="ACHJ1G_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4766918.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1855..2004
                     /locus_tag="ACHJ1G_005264"
     CDS             1855..2004
                     /locus_tag="ACHJ1G_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO4766919.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2780..>2920
                     /locus_tag="ACHJ1G_005265"
     CDS             2780..>2920
                     /locus_tag="ACHJ1G_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4766920.1"
                     /translation="MAIPYEPYGDLTMTYKYNPFWQQRIRETVRHALDVHPRLTALRV
                     DLR"
BASE COUNT          751 a          691 c          786 g          692 t
ORIGIN      
        1 tgcgtggtgg tgatgaatca acaggcatat aactgatacc cgccggtcag aagttccagc
       61 ctgcgacttt cctgtctgcg tgcccgcagt ccggcgttca ggtgcagcgt gtgacagaac
      121 gcacggagag cgaaacggca ggcagacagg ccgttcagtc agccctggca ccctgcccgg
      181 ataccagctt ccctgaaaca actggcatca ggtgcgctta tcgtcaaccg ttgtgcggta
      241 tgccaggagc acgtactgat ccatcagatc cgtcagtgct gcgctctgct tctgacggtt
      301 gatgatccat tcctccagct ctttttctga tttccgtttc agattctccg tccagtcgtc
      361 aaggcagttt ccggtcatta aatcaagcca gcaaagccag accagtttga ggcgcaactc
      421 cgtgccacca aactgacgga agaggctgat cagtttattt gccagcgaac gctgcctcag
      481 acgttcacat tcagacagga gaatgccttc cagcatcgga tgatgaacca gcagatgttc
      541 actggtgcat aaattcacta tcagatcatc atccaggggc ggcgtggtcc gtttgtccgg
      601 accataaatc agccggtgca gactctgggg tacgatggca atctccggtt tttcaccatt
      661 gaatgacacc cacccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      721 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcag
      781 ctcgtaacgg gtctgatact gtggtttcat tttcatattc ctcacggggg ataacaggca
      841 gcagaacgcc gccggcaggc cggagactgc gtctgctgca gatgaacggt taagaattac
      901 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcgctttt cagccatcgg
      961 gagctgcggc ccattttgat gggcgcagga aagtcaccat ccttgatgag tttgtaaaac
     1021 cacttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat ctggtcatcc
     1081 atcagtgaaa ctggggtgat catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     1141 tggtcatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     1201 taatcgggaa tgtgaccgcc aggagataaa cggggaggtt acggtggctg agccgtaccg
     1261 gtatgccggg tggttgttac gaaacgatgc ttccgcacgc gcgcgcgata aagaagggat
     1321 gattttcgtg aggaagaggg gaaaactggc gttctgataa ttgcagagtt atttacgggg
     1381 cggttacgat ggggcagacc gtgtccggtg ggcaggcgac aggtatcacc gatgggataa
     1441 gactgtctga ttggtattat aaatatataa gagagatcaa acacatgaga tcaaatacat
     1501 gagttcatat atgtgaaata aacacggtga gtgtgatcag tgggcatgat gagaacgcag
     1561 tagcaacaga ctgcgctatc cctgatagct ggcgtataac ctgcgtatat aaaacgtacc
     1621 agcaacgcac gtgaacagag tgcattaagc atccgtcgtt gacaccgacc tggtgagtga
     1681 cagtctgaga gaccggttaa gtggtggtac gggagtaatg agtttgggga gggtaccaga
     1741 atgtcagtct ggtacggtat gctgacagta tcacgttaat tgcagcataa agtgatgaga
     1801 ggctaatgga agttaaaacc agagcagttc cgtcgttgtt catcccggga gacactggaa
     1861 aaagtgattt cccacacgcg ttataagctt actccagcgg agatggaagc cttcaactct
     1921 gcggtcgatc accgactggc agaactgaca atgaacaagc tttacgatcg tgttcccgct
     1981 tcagtctgga aatatgtcat ctgagcgttc ggtaatcaca actgcatcgc gctaggtgtt
     2041 atccgctttg tgattcttat tggctcttct caggttatta aaagctgaat atgttcctgt
     2101 tgtcgggaac caggataata ccgggagcgt taagcggtat agcgacaaca aagaattcga
     2161 aatcaaaagg gaatggtgcc ggaggcgtgg gcggtaatag gctgattttc aggtattacc
     2221 ggcttacaac gtgtggttga tattgagggg ctgtgagttt cgcgtatttg tcagaaaccc
     2281 agatgagttt tcctgtatgg gacgaaataa ggcagcggca atacctggaa aatttaatat
     2341 ggtatgaagg agaacctgtc ggttacgctt aatatacacg gtacactgtc agctcagggg
     2401 atacatgaac cgttatctca ccagctacac agtatacaca cactgacgct gacagtagtc
     2461 agcaggacca caataatcag cgccagcagt atggtaacga tacggtctgt attccggtag
     2521 tagccgaagg ggtcgacaga accacaatac gggcaacaca ctgtattatt agtaatcgcg
     2581 tttccacagt ccctgcactt cttttgcgta tcaccataac tccctcctca cagccttacc
     2641 cgtaaccggt tttttacatt aaaaaacaac gctgaaaatt caaccgtcag tccggagacg
     2701 accgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cttcagggac
     2761 tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac
     2821 ctacaaatac aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
     2881 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt
//
LOCUS       DAZFDP010000315         2883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFDP010000315 DAZFDP010000000
VERSION     DAZFDP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..253)
                     /locus_tag="ACHJ1G_005266"
     CDS             complement(<1..253)
                     /locus_tag="ACHJ1G_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO4766921.1"
                     /translation="MRTSLVTREEMIEAIEQHTICISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRM"
     gene            complement(250..471)
                     /locus_tag="ACHJ1G_005267"
     CDS             complement(250..471)
                     /locus_tag="ACHJ1G_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4766922.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(1060..1617)
                     /locus_tag="ACHJ1G_005268"
     CDS             complement(1060..1617)
                     /locus_tag="ACHJ1G_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4766923.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKEEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            1786..2274
                     /locus_tag="ACHJ1G_005269"
     CDS             1786..2274
                     /locus_tag="ACHJ1G_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4766924.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHRVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSSANNEPPMDFDDDI
                     PF"
     gene            2472..>2883
                     /locus_tag="ACHJ1G_005270"
     CDS             2472..>2883
                     /locus_tag="ACHJ1G_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFO4766925.1"
                     /translation="MNNMTTKELLTALPKYKSHKTVRASKIKDIEIIALMDVVLLCNI
                     EVVEPEGVKVHVDKMFLQKHRPEIGGYLVAYEDGSLSYSPEKTFEEGFSRTNDFFENG
                     ALLSIEGHNGVTFITARDVTISAGGIATLQEEIDL"
BASE COUNT          808 a          700 c          690 g          685 t
ORIGIN      
        1 tcattctgtg tgcctccttg cgtgcttcgg ctgcgacggt tgcgtaattc agatgaccct
       61 gttcgagcag gtattcgcgg atatcagaca acaggatacg gtgaaccgcg ttcttgtcct
      121 ttctccggta aagttgtttg gtgatcatga agtagttggc aataacgcca ggtatatccc
      181 tggtgctgat acagattgtg tgctgttcaa ttgcctcgat catctcttca cgggtgacta
      241 atgacgttct catagtccct cctgagcaga agcgttaaca gggaggcacc agtaactgag
      301 agaattgcgt gaatcagtgg aaaaacgggc agagaaaata catggggcgt caggaagctg
      361 agagcgggcc tcatcttctg ttggtgcaat aacgaagtga tagtggcgtt tctggcagga
      421 gtaaaagcgc cagataaatt cagggcgtgc gcaaggattg gcattaacca tagttacggc
      481 ctcacaatca ggtttaacaa cctgctaccc gctgtcaaac aggtggcagg acgtgacagg
      541 gttgacagac tggcgattgt gaaaccagca ggccgaagcc tccccatcac gccccaccat
      601 aatttgggcg taacgcggtt taacggacac aaaaataccg caatatcgga aatctgcggt
      661 tgtccgcaca atcattcagg ctgtcaaacc tggtcgcaga atttgctacg acggcggaac
      721 tataagcctg aacgacgatg agatcaatag agccgtgttc gatggtaggt tatttgctct
      781 aataaatcaa atttattaga gcaataatgg ttaaatcatc ttcgcctgcg tgccatatat
      841 ttgcgcaccg cgcggcgtgg acaatctgaa gcggtttctt tctgctgcat caatctcgca
      901 gccatgctca aaaatgtcag gcacagccga agcccggcat ataatagcgg ttccagtggc
      961 cacgtctcat tgagcacata taccgccatg aaaatcgagt cgaaaactat cgccgccagc
     1021 gataacttca ttgtcgaaag tcggcggagc tgccggagtt tattcattga tcagccccgt
     1081 caggcaaagc tggcgttctt tttcacggcg aatctttaaa cctcgcaggg gcacgccgtt
     1141 actgttcacg aaatcaggga gatggttaca catattcacc cattcctctt tctgcgccca
     1201 cttgtggatg gacgtttcta ctcgcatgcc tcgcgctttg ctgtagtagg tccgtaagct
     1261 attgcatccc atattgaatg ccgcgcttgt cattgcactg aaggcattat cgggcatgtc
     1321 tttgccccga aagtgctgat taatacagcg ttcagcgatc aggatattct tttcccaatc
     1381 agcggcgatt tgctggtcgg tttttcgcac acccggcgtt accccgtgtg tattaccgat
     1441 cccgtcagtc catacccccg ccgggcacat gtatggatca cgtcggcaac cttcagcgtt
     1501 accaatcagc tcaagccccg cctggttggt tcgcacattg ccattaccca tcacgatggt
     1561 aatcatcacc gcgatagcgc aaattgcacc gcctcctgcg gctgtttttc ccttcataaa
     1621 gacctcataa gcgaattttt tacgctccag gacaacaccc attcacagcc aataccgact
     1681 gactcgatcc ctttagaagg cacaggataa tgcaaatcac ttgttagcta cgtttcaaag
     1741 atatacatta ttgctctaat taatttattt tattaggtaa gataagtggc acaacgcggt
     1801 gtaaacaaag tcatcctgat tggtaccctg gggcaagacc cagagatcag gtatatacca
     1861 aatggcggag cggtcggaag actcagcatc gcaacgaatg aatcatggcg cgacaagcaa
     1921 acgggccaac agaaagagca aacagaatgg catagagtcg ttttgttcgg gaaacttgct
     1981 gaaattgcga gtgagtattt acgaaaaggt tctcaggtct acatcgaagg gaaacttaaa
     2041 acccgtaagt ggacagatga cgccggtgta gaacgttaca cgacggaaat tatcgtcagc
     2101 cagggcggaa ctatgcaaat gatcggcgcc cgccgcgacg attcacagtc ctcaaatggc
     2161 tgggggcaat caaaccaacc tcaaaaccac cagcaataca gcggtggcgg caaacctcag
     2221 agcagcgcca ataacgaacc tccaatggac tttgacgacg atattccgtt ttgaatgagt
     2281 aaaaaacgat tgaaagaaaa gcggtggtcc agacgccaac aaaagcacga actcgctaaa
     2341 aaacgccaaa gttggcaatg gcacgcgctt ttcacgaaaa gaacaccccg agatattgct
     2401 ttcgctggtg ggaaaacatt cctgacccac ctgaaggcgc aatacaccag gttttaagca
     2461 gaggaaagac tatgaataat atgacaacga aagagctttt gacggcactt ccaaaataca
     2521 aaagccataa aacagttcgt gcctcaaaaa tcaaagatat cgaaattatc gccctaatgg
     2581 atgtggtcct tctttgcaac atcgaagtcg ttgagccaga aggggtaaaa gtccatgttg
     2641 ataaaatgtt tttgcaaaaa caccgaccag aaattggcgg atatctggtc gcttatgagg
     2701 acggatctct gtcctattca ccagaaaaaa catttgaaga aggctttagt cggactaacg
     2761 acttcttcga aaatggggca ttgctcagca ttgaaggtca caatggggtg acattcatta
     2821 cagccaggga cgtaactatt tctgctggcg gtatcgctac tctacaagaa gaaatcgacc
     2881 ttg
//
LOCUS       DAZFDP010000316         2863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFDP010000316 DAZFDP010000000
VERSION     DAZFDP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..1462
                     /gene="nhaA"
                     /locus_tag="ACHJ1G_005271"
     CDS             296..1462
                     /gene="nhaA"
                     /locus_tag="ACHJ1G_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4766926.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1528..2427
                     /gene="nhaR"
                     /locus_tag="ACHJ1G_005272"
     CDS             1528..2427
                     /gene="nhaR"
                     /locus_tag="ACHJ1G_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4766927.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2466..>2863)
                     /locus_tag="ACHJ1G_005273"
     CDS             complement(2466..>2863)
                     /locus_tag="ACHJ1G_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4766928.1"
                     /translation="DTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFE
                     PVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQI
                     PLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          683 a          632 c          713 g          835 t
ORIGIN      
        1 aagctggatt atttttgaaa tattgtccta acaaataacg ccgactgaca agaaattaat
       61 tattactttt cctaattaat ccctcagaaa tcctcacctt aagctatgat tatctgggct
      121 tagggtcact cgtgagcgct tacagccgtc aaaaactcat ctcaccgctg atggcgcaaa
      181 ttcttcaatg gatcgtaaaa aacgagttat tcctacacta taatctgatt ttaacgatga
      241 tttgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa
      301 acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta tcattgccgc
      361 tatcctggcg atgattatgg ccaatagcgg cgcaaccagt ggatggtatc acgactttct
      421 ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca tgctgttatg
      481 gataaatgac gcgctgatgg cggtattttt cctgttagtc ggtctggaag ttaaacgtga
      541 actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccagtta tcgccgctat
      601 tggtgggatg attgttccgg cattactcta tctggccttt aactatgccg atccgattac
      661 ccgcgaaggg tgggcgatcc cggcggctac tgacattgcc tttgcacttg gtgtgctggc
      721 gctgttggga agtcgtgttc cgttagcgct gaagatcttt ttgatggctc tggctattat
      781 cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact tatcgatggc
      841 ctctcttggc gtcgcggctg tagcaattgc ggtactcgcg gtattgaatc tgtgtggtgt
      901 acgccgcacg ggcgtctata ttctggttgg cgtagtgttg tggactgcgg tgttgaaatc
      961 gggggttcac gcaaccctgg cgggggtaat tgtcggcttc tttattcctt tgaaagagaa
     1021 gcatgggcgt tctccagcga agcgactgga gcatgtgtta cacccgtggg tggcgtatct
     1081 gattttgccg ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggtg tcacgctgga
     1141 tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg gcaaaccgct
     1201 ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg
     1261 aacgacttat cagcaaatta tggcggtggg gatcctgtgc ggtatcggtt ttactatgtc
     1321 tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta actgggcgaa
     1381 actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct ggttacgcgt
     1441 tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac ggctcccttt
     1501 tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca cttgtattac
     1561 ttctggcatg tctataaaga aggttccgtg gttggcgcag cggaggcgct ttatttaact
     1621 ccacaaacca ttaccggaca gattcgagcg ctggaagagc gcctgcaagg caaattattt
     1681 aaacgcaagg gacgtggtct cgaacccagc gagctgggag aactggtcta tcgctatgcc
     1741 gataaaatgt tcaccttaag ccaggaaatg ctggatattg tgaactatcg caaagaatcc
     1801 aatttattgt ttgacgttgg tgtggctgat gcactttcca aacgcctggt cagtagcgta
     1861 cttaacgccg cagtggtaga aggcgagccc attcatcttc gctgcttcga atccacccac
     1921 gaaatgctgc tggagcaatt aagtcagcat aaactggata tgatcatttc tgactgtccg
     1981 atagactcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt
     2041 ttctggtgta caaatccacc accagaaaaa ccgttcccgg cttgtctgga agaacggcga
     2101 cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc
     2161 cagggattaa acgtagaaat cctcggcgag tttgatgatg ccgcgttgat gaaagctttt
     2221 ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga cttttatgcc
     2281 gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta ccatgctatt
     2341 tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac ggattattct
     2401 gcgcttttta gtccagcggc gcgttaatcg gcacctcccc ccaaagttaa ggtgggggag
     2461 atagattagt tgtacataac cacgattttg actcggctca ttatttgccc gcttgagaca
     2521 ttatttccat atgtacgcgg gcgaataaat agaggaatct gattacttcc ttcatgggga
     2581 tgctgaaaag agtagtaatt gcttttacca tactggattt cattcgtacc atcattggag
     2641 agttgaaagc ctatttgacc cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg
     2701 ctggttcctg actcctgtat cgtcactggt tcaaagtaat attcaacatc actgggttca
     2761 aaaactaatt caccttgctc gccctggatg cagtcaaatc ttatatgatg aaactgataa
     2821 ttttcattat tgatacgaga cagtacaaat cgtccagtgt cga
//
LOCUS       DAZFDP010000317         2829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFDP010000317 DAZFDP010000000
VERSION     DAZFDP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..273
                     /locus_tag="ACHJ1G_005274"
     CDS             91..273
                     /locus_tag="ACHJ1G_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766929.1"
                     /translation="MKWYTPEHVVEAFKKGELTRHQVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            421..1086
                     /locus_tag="ACHJ1G_005275"
     CDS             421..1086
                     /locus_tag="ACHJ1G_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4766930.1"
                     /translation="MNQNTKTIAEAKAQCLNKKDSKQTYVVVEQWLKMFKKWFSPSGK
                     EYTATLPAVEIYSYVAGFNEQGQECYANQKTLANIGMVSDRQVRNIINMFIDMGVLSV
                     EARDGQTSLYKALPFTDAHITPPEDVKTSVNVEQPAPETQEPLEATHSIEQETQAEIT
                     EQSDLFTWLISIDSQKPMQEETENFIKRALTSKRIILEDDAFNALMIALRKESEIYDS
                     IPF"
     gene            1099..1356
                     /locus_tag="ACHJ1G_005276"
     CDS             1099..1356
                     /locus_tag="ACHJ1G_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766931.1"
                     /translation="MKYNLTLNKRTFEITESGYTLAKQLADSNYELPAIISFQELPHS
                     KEHTQHWLNHLSHFGVIKYEAVNSQTFRLLEISPFELWVAL"
     gene            1353..1559
                     /locus_tag="ACHJ1G_005277"
     CDS             1353..1559
                     /locus_tag="ACHJ1G_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766932.1"
                     /translation="MTIFDSEFSQETFLQYLRERTGMILFDSEFSGVVGEQFLYALLD
                     SLSKQGLLQYTKTHMKAFRIDSVA"
     gene            1681..2061
                     /locus_tag="ACHJ1G_005278"
     CDS             1681..2061
                     /locus_tag="ACHJ1G_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766933.1"
                     /translation="MSKKKGKKNVITTIGEACDQYALNNPRKYNYEHDAQPGEELIFI
                     LEEPSPELTDEELSLIQSMDGVYFNPRPRKTTVIDSSLLKRWQCLGFQDMHHFRGYMA
                     LNSLQYAGVRETELLLLDKIKIDD"
     gene            2103..2324
                     /locus_tag="ACHJ1G_005279"
     CDS             2103..2324
                     /locus_tag="ACHJ1G_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766934.1"
                     /translation="MINKAILETTTFHDLKLLSECFISLIERAQEENDLGLLDDIIDN
                     IATYKIPLTIAMKKAREAQQQLEQSEEVA"
     gene            2324..2632
                     /locus_tag="ACHJ1G_005280"
     CDS             2324..2632
                     /locus_tag="ACHJ1G_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766935.1"
                     /translation="MSEVHNVTIQMMDLVIDLQKAVNAALTFPSELTITQAVINLEST
                     REALQLLIAACEESEGAQIEADDLSNLIIVIHDTTEIMKEAVADLQPLINELIDKANN
                     "
     gene            2632..>2829
                     /locus_tag="ACHJ1G_005281"
     CDS             2632..>2829
                     /locus_tag="ACHJ1G_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766936.1"
                     /translation="MTKTKRVRLLALTEEGKAGNCFTVGKVYTGLLDTYTRLMHVTDD
                     NGNDQTIVLDGNCVFGEWELTT"
BASE COUNT         1011 a          533 c          581 g          704 t
ORIGIN      
        1 tccatctaaa taatcaaatc acagaacact gcactttgag ccgtcaggct gcaaggtgtg
       61 tttttctttt atctaaataa ggagtttttt atgaaatggt atacaccgga acacgtcgta
      121 gaggcattta aaaaaggtga gctaacacga catcaagtaa ggatgaacag gaacacagcg
      181 cggcgctgtg gttatccaga gcgtgaaaaa tgctttgacg atgcactaaa aattattgat
      241 gaactgcgta aggcagaagc agagaaagaa taataacagg agaaggagaa aaccaaatga
      301 atattaattt tgattctgca acttataacg cgttgttaaa tcgtgcaaag gcagaaggga
      361 aatcaattcc aaaacttgtt aatgaattac taattacagt ccttacaaag gaggaactaa
      421 atgaaccaaa acaccaaaac aattgccgaa gcaaaagcgc aatgcctcaa taagaaagat
      481 agtaaacaaa catatgtagt agttgaacaa tggctgaaga tgtttaaaaa gtggttctca
      541 ccgtcaggta aggaatacac cgcgacactt ccagccgttg aaatctacag ttacgtagct
      601 gggttcaatg aacaagggca agaatgttac gccaatcaaa aaacattagc taatattgga
      661 atggttagtg atcgtcaggt gcgtaatatt ataaatatgt ttattgatat gggtgttctt
      721 tctgtagagg cacgggacgg gcaaacatca ctctataaag cattaccctt tacagatgca
      781 cacatcacac caccggaaga cgtaaaaacc tctgtaaacg tagaacagcc tgcacctgaa
      841 acacaggaac cgttagaagc gacacactct attgagcagg aaacacaggc agaaatcacg
      901 gaacaatctg atctttttac atggcttatt tccatagaca gccaaaagcc tatgcaagaa
      961 gaaacagaaa attttataaa acgtgcactg acctcgaaga ggataattct tgaagatgat
     1021 gcttttaatg cactgatgat agccctgaga aaagaaagtg aaatctatga ctcaatacca
     1081 ttttaaggag aataaaaaat gaaatacaac ctgacattaa acaaacgaac attcgaaatt
     1141 acagaaagcg gctacacact ggcaaaacag cttgctgata gcaattatga gctacccgcg
     1201 attatcagct ttcaggagtt acctcatagt aaagaacaca cacagcactg gttaaatcac
     1261 ctgagccatt ttggggtaat taagtatgag gctgtgaata gtcagacgtt ccgtttgctt
     1321 gagataagcc ctttcgaatt gtgggtggcg ctgtgaccat attcgatagc gaattttcac
     1381 aagaaacctt tctgcaatat ttacgggaaa gaacgggaat gattctgttt gattctgagt
     1441 tttccggtgt ggtaggtgag caatttttat atgctcttct cgatagccta agtaaacaag
     1501 gattattgca gtataccaaa actcatatga aagcattcag aatcgactct gtagcttaaa
     1561 acaaacaact atcaaacatc ttgaagggtg aaacaatgga aagcttaata tttttcttag
     1621 tatttatgat cgcttccttt tttaacactg caacaagaaa aacaaaacga aggggaagca
     1681 atgagcaaga aaaaaggcaa gaaaaacgta ataacaacta ttggcgaagc atgtgatcag
     1741 tatgcgttaa acaatcctcg caaatataat tatgagcatg atgcacaacc aggcgaggaa
     1801 ttgatcttta tacttgaaga accaagccca gaattaacag acgaagaatt atcactcatt
     1861 caaagtatgg acggtgttta ctttaatccg cgcccacgca aaacaacggt tatagattcc
     1921 agtcttctga aaagatggca gtgtttgggt tttcaggata tgcaccactt tagggggtat
     1981 atggctctta acagtctgca atatgcaggg gtgagagaaa ccgaattact tctgcttgat
     2041 aaaataaaaa tcgacgatta acagcgtttg ctgaatataa cccttaacaa aaggaaaata
     2101 aaatgattaa caaagcaatt ttagagacaa ccacttttca cgatcttaaa cttctttctg
     2161 aatgcttcat aagcctgatt gaacgtgcac aggaagaaaa cgatctcggt ttactcgatg
     2221 atattataga caacattgca acttataaaa tcccgctcac tattgctatg aagaaagctc
     2281 gtgaagcaca acaacaatta gaacaatcag aggaagtggc ataatgagcg aagtacacaa
     2341 cgtaactatt caaatgatgg atctggttat tgatttacag aaagcagtaa acgcggctct
     2401 gacttttcca agcgagctaa ccatcacaca ggcggttatt aatctggaat cgacgcggga
     2461 agcactgcaa ctacttattg cagcgtgcga agagagcgaa ggtgcacaga ttgaagctga
     2521 tgacctttct aatctgatca tcgtcattca tgacaccact gaaatcatga aagaagcggt
     2581 agcagatctg caacccctca ttaatgaatt aatcgataag gctaacaact aatgacaaaa
     2641 actaaacgtg tgcgccttct ggcgctcaca gaagaaggga aagcgggtaa ttgtttcaca
     2701 gtaggtaaag tgtataccgg cttactcgac acttacaccc gcctgatgca cgtaaccgat
     2761 gataacggta acgatcaaac aatcgtttta gatggtaatt gcgtatttgg tgagtgggaa
     2821 ctgacaacc
//
LOCUS       DAZFDP010000318         2795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFDP010000318 DAZFDP010000000
VERSION     DAZFDP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2795
                     /gene="ehxA"
                     /locus_tag="ACHJ1G_005282"
     CDS             <1..>2795
                     /gene="ehxA"
                     /locus_tag="ACHJ1G_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4766937.1"
                     /translation="AFNTASSSVRSAGKKLILLIPDNYEAQGVGINELVKAADELGIE
                     IHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIGRTAENV
                     GNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKSSIELINQLVD
                     TVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGSLGDGLDVVSGILSA
                     VSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTTAASAGL
                     ITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKETGAIDAA
                     LTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFEHVAEKF
                     AARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQQHWDEK
                     IGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLSTGNVSS
                     VLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDYSKLIQF
                     VEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHDTVSYNKTDVGKLTIDATGA
                     SKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVIDNLH
                     SVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQGDDQ
                     LFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGIDLLDGG
                     EGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVGNDLIMN
                     KAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKIPHNNNE
                     RSGYIKASNIASEKNMV"
BASE COUNT          905 a          433 c          683 g          774 t
ORIGIN      
        1 cagcatttaa tacagcatca tcaagcgtac gttccgctgg caaaaaactc atattattaa
       61 tacctgataa ttatgaagct cagggcgtgg gtattaatga gttggtcaaa gctgctgatg
      121 agcttggaat agaaatacac cgtactgaac gagatgatac agcgattgca aaccagtttt
      181 ttggtgcagc agaaaaagtt gtaggattaa ctgaacgtgg tgttgcaata ttcgcaccac
      241 aacttgacaa acttctgcag aagtatcaga aagttgggag taaaatagga agaaccgctg
      301 aaaatgtagg taataatctg ggaaaagccg gaacagttct ctcagcacta cagaatttta
      361 cggggattgc tttatcaggc atggctcttg atgaattgct gagaaaacaa cgggaaggag
      421 aggatataag tcagaatgat attgccaaaa gtagtattga acttattaat cagcttgtag
      481 atacagtatc aagtataaac agtaccgttg attcattttc tgagcagctt aaccagcttg
      541 gctcattttt atccagtaaa cctcgcttaa gttctgttgg tgggaaatta caaaatttac
      601 cagacctggg ctccctgggg gatgggctgg atgttgtctc cggaattctt tctgctgtat
      661 cagcaagctt tattctggga aacagtgacg cacatacagg aacaaaagct gcagcgggta
      721 tcgaactgac aactcaggtt cttggaaatg ttggtaaagc tgtttcgcaa tatattctgg
      781 ctcagagaat ggcacagggg ttatcgacaa cagctgcaag tgcgggtctg atcacatcgg
      841 ctgttatgct ggctatcagt cctctttctt tcctggctgc tgcagataaa tttgagcgag
      901 ctaagcagct tgaatcatat tctgaacgat ttaaaaaatt gaattatgaa ggggatgctt
      961 tactcgcagc ctttcataaa gaaaccggag ctatagatgc agccctgaca acaataaata
     1021 ctgtcctgag ttctgtatct gcgggagtta gtgcagcctc cagtgcatcc ctcatagggg
     1081 ccccgataag catgctggtg agtgcattaa ccggtacgat atctggcatt ctggaagcat
     1141 caaaacaggc tatgtttgag cacgttgcag agaaattcgc tgctcggatc aatgaatggg
     1201 aaaaggagca tggcaaaaat tattttgaga atggctatga cgcaagacat gctgcgtttt
     1261 tagaagactc tctgtctttg cttgctgatt tttctcgtca gcatgcagta gaaagagcag
     1321 tcgcaataac ccagcaacat tgggatgaga agatcggtga acttgcaggc ataacccgta
     1381 atgctgatcg cagtcagagt ggtaaggcat atattaatta tctggaaaat ggagggcttt
     1441 tagaggctca accgaaagag tttacacaac aagtgtttga tcctcaaaaa gggaccatag
     1501 acctttcaac aggtaatgta tcaagtgttt tgacatttat aacaccaaca tttaccccag
     1561 gagaagaagt tagagaaaga aaacagagtg gtaaatatga atatatgaca tctcttattg
     1621 taaatggtaa ggatacatgg tctgtaaaag gcataaaaaa tcataaaggt gtatatgatt
     1681 attcaaaatt gattcagttt gttgaaaaga ataacaaaca ctatcaggcg agaatgattt
     1741 ctgaactcgg agataaagac gatgtggttt attctggagc aggctcatca gaagtatttg
     1801 ctggtgaagg tcatgatacc gtatcttata ataagacgga tgttggtaaa ctaacaattg
     1861 atgcaacagg agcatcaaaa cctggtgagt atatagtttc aaaaaatatg tatggtgacg
     1921 tgaaggtatt gcaggaagtc gttaaggaac aggaggtgtc agtagggaag cgaacagaga
     1981 aaatacaata tcgtgatttt gaattcagaa ccggtggaat tccttatgat gtaatagata
     2041 atcttcattc tgttgaagag ctcattggcg gaaaacatga tgatgaattc aaaggcggta
     2101 agtttaatga tatattccat ggcgcagatg ggaacgatta tatcgaaggt aattatggta
     2161 atgatcgact atacggcgat gatggggatg attatatatc cggaggacag ggagacgacc
     2221 agttatttgg tggtagtgga aacgataaat tgagtggagg ggatggtaat aattatctga
     2281 caggaggaag cggtaatgat gagcttcagg cacacggagc ttataatatt ctgtcaggtg
     2341 gtactggtga tgataaactt tatggtggtg gtggtattga tcttctggat ggaggggaag
     2401 gtaatgacta tctgaatggt ggttttggta atgatattta tgtttatagg caaaactatg
     2461 gtcatcatac aattgcagat gaaggaggta aaggagatcg tttgcactta tctgatatta
     2521 gctttgatga tatcgcattt aagagagttg gaaatgatct tatcatgaat aaagccatta
     2581 atggtgtact ttcatttaat gagtcaaatg atgtcaatgg gataacattt aaaaactggt
     2641 ttgcgaaaga tgcctcagga gcagataatc atcttgttga ggttataaca gataaagatg
     2701 gtcgagagat aaaagttgat aaaatacctc ataataataa tgaacggtca ggttatataa
     2761 aagccagtaa tatagcatct gaaaaaaaca tggtt
//
LOCUS       DAZFDP010000319         2787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFDP010000319 DAZFDP010000000
VERSION     DAZFDP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHJ1G_005283"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHJ1G_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO4766938.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..>2787
                     /gene="safA"
                     /locus_tag="ACHJ1G_005284"
     CDS             2636..>2787
                     /gene="safA"
                     /locus_tag="ACHJ1G_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4766939.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGR"
BASE COUNT          734 a          656 c          679 g          718 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcat
//
LOCUS       DAZFDP010000320         2780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFDP010000320 DAZFDP010000000
VERSION     DAZFDP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..187
                     /locus_tag="ACHJ1G_005285"
     CDS             <1..187
                     /locus_tag="ACHJ1G_005285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766940.1"
                     /translation="PCKGGALPAELITPAVWSRIIGRVQNESTHFLKKLFVRRKFKQD
                     DRKTDRVAQFVNENNNA"
     gene            300..1715
                     /gene="gltX"
                     /locus_tag="ACHJ1G_005286"
     CDS             300..1715
                     /gene="gltX"
                     /locus_tag="ACHJ1G_005286"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4766941.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1767..2159)
                     /locus_tag="ACHJ1G_005287"
     CDS             complement(1767..2159)
                     /locus_tag="ACHJ1G_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4766942.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2161..2520)
                     /locus_tag="ACHJ1G_005288"
     CDS             complement(2161..2520)
                     /locus_tag="ACHJ1G_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4766943.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          677 a          672 c          703 g          728 t
ORIGIN      
        1 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cccgctgtgt ggagtcgcat
       61 tatagggaga gttcaaaatg agtcaacgca ttttctaaag aaattgttcg ttcgtcgtaa
      121 atttaagcaa gatgatcgca aaacagaccg tgttgcgcaa tttgtcaacg aaaacaataa
      181 tgcgtaaggt tgcaacccga actacattga ggaatcaggc gggagtgata gaatatcgcc
      241 cactcaattt ttccaggatt tgccggttgt cggcatcttt ctaaacgtaa ggccatttca
      301 tgaaaatcaa aactcgcttc gcgccaagcc caacaggcta tctgcacgtt ggcggcgcgc
      361 gtactgctct ttactcctgg ctttttgcac gtaaccacgg cggtgagttc gtgctgcgta
      421 ttgaagacac cgatcttgag cgttccacgc cggaagctat cgaagccatt atggatggca
      481 tgaactggct gagcctggag tgggatgaag gtccgtacta ccagaccaaa cgttttgatc
      541 gctacaatgc ggtgatcgat cagatgctgg aagagggcac tgcttataaa tgctattgct
      601 ctaaagagcg cctggaagcg ctgcgcgaag agcaaatggc gaaaggtgag aagccgcgtt
      661 atgacggtcg ctgccgccac agccatgagc atcatgctga tgatgaaccg tgtgtcgtgc
      721 gttttgctaa tccgcaggaa ggttctgttg tttttgacga tcagatccgt ggtccgatcg
      781 agttcagcaa ccaggagctg gatgatctga ttatccgccg taccgatggt tccccaacct
      841 ataacttctg tgtggttgtc gatgactggg atatggaaat cacccacgtt atccgtggcg
      901 aagaccatat caacaacacg ccgcgccaga tcaacattct taaggccctg aaagcgccgg
      961 tgccggttta cgcgcacgtt tctatgatca atggcgatga cggtaaaaaa ctgtccaaac
     1021 gtcacggggc agtcagcgta atgcagtatc gtgatgacgg ttatttgcca gaagcactgc
     1081 tgaactatct ggtgcgtctg ggctggtccc acggcgatca ggaaatcttc actcgtgaag
     1141 agatgatcaa atacttcact ttgaatgccg tcagcaaatc tgccagtgcg ttcaacaccg
     1201 acaagctgct gtggctgaac catcactaca ttaacgcgct gccgccggag tatgttgcta
     1261 ctcacttaca gtggcacatt gagcaggaaa atatcgatac ccgtaacggc ccgcagctgg
     1321 ctgatctggt gaaactgctg ggcgaacgct gcaagacgct gaaagagatg gcacagagct
     1381 gccgttattt ctacgaagat tttgctgagt tcgatgccga tgcggcgaaa aaacatctgc
     1441 gtccggtagc gcgtcagccg ctggaagtgg ttcgtgacaa actggccgcg attactgact
     1501 ggaccgctga aaacgttcat cacgctattc aggcgacggc ggatgagctg gaagtgggta
     1561 tgggtaaagt tggtatgccg ctgcgtgtcg ccgtaaccgg tgcggggcag tctccagcac
     1621 tggatgttac cgttcacgca attggtaaga cccgcagtat cgagcgtatc aacaaagcgc
     1681 tggattttat tgctgaacgc gaaaatcagc agtaattagc gtgtaaaaag ataaacggca
     1741 ggagataata tcctgccgtt ttttatttat gctgattcgt caatatcgag acgggcgagg
     1801 aaattgcgaa ttccttcacg tgacaaaaat tgcgcgactt tttcctgctc aatgtctgtc
     1861 atattatcta tcgcgttgat gatttgtcga tacgccgggg cgcgtgctcc aggagcgtat
     1921 tcagcaattc tctcttcagc ttccatcaac attggtgtgt tatggaaggc tggcgtgttc
     1981 tgaataaact cgcaaacttg cgtatcgacc agaataagtc tggcacgacc gcctttcacg
     2041 ccgggaaatt tttccgtttt ccagcctttt tctctgatcc aacggttaac agtttgttta
     2101 gccacaccga ggcattcagc cagttcttcg gtggtcattt tgttgcgtaa tcttttcata
     2161 ttatttgcta tcgcgaatcc ctaagcgttg taacaatccg ataatccctt cccgcagaag
     2221 caaggatgta aactgttttt gttcaactgg cgtcatctct ttcgccagtg tcaccagcaa
     2281 tacttcaagc ggcgcatcac cagaaagggc aggcgcttct ccctgacctt ctggacgttc
     2341 agcattgcga atatattcac gaacctgttc attgacgtga accagtcggg ctttgccacc
     2401 ctggacgcca ggttttggtg acgttgtcca gccttccttg cgtacccatt tattaatggt
     2461 ctggcggcta tagccggtca gtctggcaag ttcatctggt gtcatcctct ccttgaacat
     2521 aacaattccc tgaatgtatg tgggtctatt agtagttcat tttataacac cactttacag
     2581 gaaaccgtga catgaataac tactgaatgc gagcggtttt ccaaaattgt tcatttgcct
     2641 gatttttcag cgattgaaac tacacgttgc aatttgccgt tgacacattc gggcggaatt
     2701 catatgatgc cgcccgtcaa ctcgacaagc tttacgtgac ggggctatag ctcagctggg
     2761 agagcgcttg catggcatgc
//
LOCUS       DAZFDP010000321         2778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFDP010000321 DAZFDP010000000
VERSION     DAZFDP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..671
                     /gene="comR"
                     /locus_tag="ACHJ1G_005289"
     CDS             39..671
                     /gene="comR"
                     /locus_tag="ACHJ1G_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4766944.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ACHJ1G_005290"
     CDS             complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ACHJ1G_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4766945.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1499..2776)
                     /gene="ndh"
                     /locus_tag="ACHJ1G_005291"
     CDS             complement(1499..2776)
                     /gene="ndh"
                     /locus_tag="ACHJ1G_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4766946.1"
                     /translation="MVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHE
                     VATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPER
                     KIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANG
                     KVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPR
                     ISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDI
                     GGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMN
                     NILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLY
                     RMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          666 a          768 c          684 g          660 t
ORIGIN      
        1 ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat
       61 gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg
      121 ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag
      181 cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc
      241 gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt
      301 gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct
      361 ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc
      421 cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt
      481 tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga
      541 cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag
      601 gtgcatcgct ggaaaaacta atgcagattg ccggaacgac tttgcgttta tggcccgaac
      661 tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc caaattgcag
      721 agtacagcct tgttatactt tgttattcaa aatcagttgc ccattactat ctagcgggat
      781 ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt
      841 cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt
      901 agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc
      961 acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccgccaa actgatgccc
     1021 tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac
     1081 cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc
     1141 cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa acacgttcag
     1201 actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac ccgccaacat
     1261 tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag
     1321 aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata cttaatgatt
     1381 cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct
     1441 cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg tacgtcgatt
     1501 aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc
     1561 cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa
     1621 agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac
     1681 caacggtgga gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt
     1741 tcagcggctt accattcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt
     1801 gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt
     1861 cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc accaccagct
     1921 ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc
     1981 cggctgccca caccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc
     2041 cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct
     2101 cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt
     2161 ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt
     2221 gcttgaccgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa
     2281 tcgcaatgtt cactttgcca tttgcgccca ggttggcgga gtatttcagg aagagattca
     2341 gcatctcctg gtgaaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt
     2401 tgacacctgg cgtattgaaa tcgttagagg tgctacccag cgccattacc agggtgtcat
     2461 aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt tctgcaatag
     2521 tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac tggaaaccat
     2581 gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg
     2641 ccacttcgtg cagcagcggt ttccacagat ggctgtggtt acgatcgacc agcgtaattt
     2701 tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag
     2761 caccgccgcc gacaatca
//
LOCUS       DAZFDP010000322         2769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFDP010000322 DAZFDP010000000
VERSION     DAZFDP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..179
                     /locus_tag="ACHJ1G_005292"
     CDS             15..179
                     /locus_tag="ACHJ1G_005292"
                     /inference="COORDINATES: protein motif:HMM:NF022255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO4766947.1"
                     /translation="MEEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALN
                     IIIDITSGDI"
     gene            complement(499..630)
                     /locus_tag="ACHJ1G_005293"
                     /pseudo
     CDS             complement(499..630)
                     /locus_tag="ACHJ1G_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..2270
                     /locus_tag="ACHJ1G_005294"
     CDS             912..2270
                     /locus_tag="ACHJ1G_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO4766948.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2509..>2769)
                     /locus_tag="ACHJ1G_005295"
     CDS             complement(2509..>2769)
                     /locus_tag="ACHJ1G_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4766949.1"
                     /translation="LAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYSLI
                     GTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          727 a          666 c          709 g          667 t
ORIGIN      
        1 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
       61 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
      121 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
      181 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
      241 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
      301 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcattttgg tatcatgggc
      361 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
      421 ttaacccggt gtatcgttat gatcttatcc agcaattgaa ccgccccggg tttcctggag
      481 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
      541 tttttctgct tctgccggtg gaatatggcc ctgcctttcc agcaatcgtc gattgttata
      601 ccagtccacc cacgtgagtg tggtcagctc atcatactga cacgtgcatt acccctaccg
      661 cgataatgca accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc
      721 aacaagacac agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt
      781 tgttacaaat aacaacagtg ttttatattt ctttggcctc aggcccgcgc atgttgccat
      841 tcgatgtcat tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatgg
      901 atgctaacaa tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct
      961 ctgccctggc ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc
     1021 acgtgaacta tgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag
     1081 gttctggcct gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg
     1141 atcgcggtcc gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta
     1201 cgccagattt cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca
     1261 tcgatccccg tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg
     1321 acgtgattgg ctccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg
     1381 ataatcgcgg gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt
     1441 gcgatgagta tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc
     1501 acggtccgca ggcggcggaa ggggaaaaag cgattgcggg cggtttgcca aatatcctca
     1561 aatggcgtca ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta
     1621 ttgtcgccgt gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta
     1681 cgcgtctggt gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg
     1741 acagcgcggc ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata
     1801 accagcacat cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga
     1861 tttacgaggt cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt
     1921 atccggagtt tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa
     1981 cgcaggttgt tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc
     2041 tgatcgacag caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga
     2101 tgcaacatcc ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc
     2161 tgacgcttga cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg
     2221 agtctgacag tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg
     2281 tacggacagc agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc
     2341 ggcgtagacc cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc
     2401 atctcatggt ggcagccggt taactttcct gctacaccag tggaaaaagg ccataagcag
     2461 tccgaacaaa agtaagcgta cagcgagggc cgtattgacg gggatgtgtt attcagctgg
     2521 cagtgctatg cgccacggaa gcagttcgct gacccggttg accggccagt ctgctatgac
     2581 gccaggcaca tggcgaaggt agctttctgg atccacgtca ttcagtttgc acgtcccgat
     2641 caggctgtac agtagcgctc cccgctcacc accatggcca gagccgaaga acagggtctt
     2701 catcttttca cgcaaccagc tttccaggga tttcaacagc ggtttcgttt ttcgctgacg
     2761 ttcagcaag
//
LOCUS       DAZFDP010000323         2767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFDP010000323 DAZFDP010000000
VERSION     DAZFDP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..187
                     /locus_tag="ACHJ1G_005296"
     CDS             2..187
                     /locus_tag="ACHJ1G_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO4766950.1"
                     /translation="MEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDAGQEVE
                     MPEPEKKKREMIQLWGE"
     gene            190..513
                     /locus_tag="ACHJ1G_005297"
     CDS             190..513
                     /locus_tag="ACHJ1G_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4766951.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            510..902
                     /locus_tag="ACHJ1G_005298"
     CDS             510..902
                     /locus_tag="ACHJ1G_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO4766952.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHYQLPHGAYPA"
     gene            899..1306
                     /locus_tag="ACHJ1G_005299"
     CDS             899..1306
                     /locus_tag="ACHJ1G_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO4766953.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     VDNLNIISTGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNTSPLP"
     gene            1444..1911
                     /locus_tag="ACHJ1G_005300"
     CDS             1444..1911
                     /locus_tag="ACHJ1G_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4766954.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            1904..2539
                     /locus_tag="ACHJ1G_005301"
     CDS             1904..2539
                     /locus_tag="ACHJ1G_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4766955.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRMFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            2551..>2767
                     /locus_tag="ACHJ1G_005302"
     CDS             2551..>2767
                     /locus_tag="ACHJ1G_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4766956.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMII"
BASE COUNT          676 a          753 c          815 g          523 t
ORIGIN      
        1 actggaaggc gacaccgtgg attcgctctg tttccggtac tacggcacga cgcagggcgt
       61 caccgaaaag gtgctggatg ccaaccccgg actctgtcag caggtatttc tggacgccgg
      121 gcaggaagtg gagatgccgg agccggagaa gaagaaacga gaaatgattc agttgtgggg
      181 ggagtagcag tgagcaccat tcaaacaggg atcacagagc aggttattgc gtggctcttt
      241 gaccacctgc caacggtgta tgcagtaggc gcggcggtca gcatttccgc gctgatgagt
      301 ctttatgacg gacgaacact ggttcagacc gtaacgggat cgctggcgtg cggcgttctt
      361 gccatggccg tggccgggtc gttgcgcttc ttcggttttc ctgaagatgc cgtgacgttt
      421 atcggcgcat caatcggttt tatgggcgca gagaaagcac gcgacaaggt tattgcagcc
      481 tttaatcgca gggtgaagga gaaggacgaa tgagcaacac atttaaattc agcagccgga
      541 gcgaaaagaa tttgcagggc gtaaatcctg atctggtgaa agtgacccga cgggcactgg
      601 aaatttcgga agtggatttt ggtatcaccg aagggttgcg cagccgttac cgccagaagc
      661 aacttgtggc cacaggtaag agccagacca tgaacagccg ccaccttacg ggacatgccg
      721 tggatgttgt ggcttatatc ggcagccagg tgtcatggga gtggccgctg tacgaaaaaa
      781 tcgcagcagc attcagacag gccagccggg aactgaatat tccggtggaa tggggcggcg
      841 actggaagac cctgaaagac ggaccgcatt atcagttacc acacggagcc tatccggcat
      901 gaagctctgg cccacgctgg gcgtcgcttt ccttctgatt gccgcatggg gaacatccat
      961 gcgtctgtcg tggtcgctgg gccgggagaa cgccagaaac gaagcgcagg ccagcgccct
     1021 gaaaagcacc gtcgacaacc tgaatatcat cagcaccggg gtacaggata tgcagcaggt
     1081 gctggcgcaa ctccgcgtgg aaaatcaaca gcgaaatcag gacggagagg ccagacgtga
     1141 acagctacgc aacgatattg caaaagatga atgcgcccac gctttgcctg acgctcgttt
     1201 tactgacagg ttgcgcaggc acgcagaacg cgccacggcc agcgccgtca gtccggctta
     1261 taccgcagac gctgaccata ccggtaacac ctcccccctt ccctgacact cccacatggg
     1321 gaaatctcgg tatatggggc gaccgccttc tggatgcact ggaaacctgt aacgcggata
     1381 aacgggccat tgaattactg gaacagcgca gactgcaacg actgaacaac gaggacaaca
     1441 actatgctga aaactgattc cctgcgtgaa gccatgaccc gttcatgccg atggtgtcag
     1501 gccaacccgg aaaaattcac cattttcgtg gagagcggca acattgaaac gacaggagaa
     1561 accccatcgt ttgtttaccg ctatcagatg gtgatgtttg tcatggatta cgccggggag
     1621 ctggacgacc tcacgctgcc gctgctggcg tggttatccg aaaatcagcc acagttgttg
     1681 ctcaaccctg agcgtaatca ggacatcaaa ttttccgccg ttatcaatga cgatgacagc
     1741 gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc gcatcacgcg cagcagtcag
     1801 ggcacaccgc aggcagaaca cctgccggag ccaaaacccc gcctgccctc ttccgaaggc
     1861 gactggtcgc atgtattcca ggatgtgacg tggggtgaaa gcgatggata aggcattcac
     1921 ccgcgtggat gaaacctttg aggccatccg cgacagcctg aatcagcagg ccatcaataa
     1981 catcgccaga aagctggcac aggatttacg ccgcgcccag caggcgcgca tccggtcaca
     2041 gaaagcgcca gacgggaccg catggacacc acgcagacgc cgcgtaaccc ggatacagga
     2101 acgcattcgc tttatctgga ataacgaagc acgcacgctg aaaaactggc atcacgacac
     2161 ggggaaatac gggcgaacca ttaccgggtg ggatgaggat aaaaacaata tccgcatgtt
     2221 ttaccgggat gacatcgacc gttttctgga aatacgcacc cggcgcatca accaggacag
     2281 cacaaagcgc gtccccatgt tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc
     2341 agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc gcacgtattg cacgcgttca
     2401 tcagtttggt gagcgcgatc aggttgcgcc gggcattttc accgattacc cggtacgtga
     2461 gctgctgggt atcagtcagg cagatgaacg cctgatttat aacacggtgc tgggccggat
     2521 tgcggaggct gtacggtgag cgcagaactc atgcgactgc tgagcaatat catccgcacc
     2581 gggatcatct ctgaagttga cgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg
     2641 gaaacaggct ggctgcgctg gaacaccacg cgcgcgggag ccttcaatgt gtggctgccg
     2701 ccatcacctg gcgaacaggt ggtaattgcc tgcattggcg gcaacccgga aaccgccatg
     2761 ataattg
//
LOCUS       DAZFDP010000324         2737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFDP010000324 DAZFDP010000000
VERSION     DAZFDP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHJ1G_005303"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHJ1G_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4766957.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHJ1G_005304"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHJ1G_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4766958.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..>2737)
                     /locus_tag="ACHJ1G_005305"
     CDS             complement(1944..>2737)
                     /locus_tag="ACHJ1G_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4766959.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          755 a          733 c          649 g          600 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggattata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaaga
//
LOCUS       DAZFDP010000325         2694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFDP010000325 DAZFDP010000000
VERSION     DAZFDP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..188
                     /locus_tag="ACHJ1G_005306"
     CDS             <1..188
                     /locus_tag="ACHJ1G_005306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766960.1"
                     /translation="GFLHPQNIRRPPERAVRNETLFRKKLPTKEQNNRVSVFHSVYSV
                     DDTDIPAITGLDNWKDI"
     gene            109..273
                     /locus_tag="ACHJ1G_005307"
     CDS             109..273
                     /locus_tag="ACHJ1G_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4766961.1"
                     /translation="MFFILFIVLMIPISPLLLVWIIGRTFEPVIELYTDVTWESFSAL
                     HNKINPYKEN"
     gene            275..832
                     /locus_tag="ACHJ1G_005308"
     CDS             275..832
                     /locus_tag="ACHJ1G_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4766962.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1123..1278
                     /locus_tag="ACHJ1G_005309"
     CDS             1123..1278
                     /locus_tag="ACHJ1G_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4766963.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1398..1742
                     /locus_tag="ACHJ1G_005310"
     CDS             1398..1742
                     /locus_tag="ACHJ1G_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4766964.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1864..2136
                     /locus_tag="ACHJ1G_005311"
     CDS             1864..2136
                     /locus_tag="ACHJ1G_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766965.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2138..>2694
                     /locus_tag="ACHJ1G_005312"
     CDS             2138..>2694
                     /locus_tag="ACHJ1G_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766966.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          703 a          614 c          733 g          644 t
ORIGIN      
        1 agggattcct gcaccctcag aacatcagga ggccgcccga aagggcggta agaaatgaaa
       61 cattatttag aaaaaaatta cccacgaaag agcagaacaa cagagtttct gtttttcatt
      121 ctgtttatag tgttgatgat accgatatcc ccgctattac tggtctggat aattggaagg
      181 acatttgaac cagttattga gctatatacc gatgtgacat gggaatcatt cagcgcactg
      241 cacaataaaa ttaatccgta taaggaaaac tgatatgagc actattacca gagaacgcgc
      301 ggagattaaa tcatacatca caggcttcct gagcgactcg gcgcacgata acaagtcttc
      361 agacagcctg ctggctaatg tgtttcgtat cgcgctggca tcactggaag cagagccgat
      421 agcaatggta gtgcctgatg aaatggattt gcttacctgc catctcgacg gtgtaactaa
      481 aacatatgct gatggctgga acgcctgccg cgtcgccatg cttcaggccg gaaactttcg
      541 ggaaaataag aattcgtcaa ccaacaattt tcgggaaatc tcggaaacgt caaccagatc
      601 tccgataact ctggatggct ggataagctg tactgagcga atgcctgaaa agagccagaa
      661 cgtgcttatt tcgatgaata tcgatagcga ggctgggcca ttaatatatt ccgcacgcta
      721 tctcggaggc acgttccggc gcggaggtat agcagttagt ccgggtaatg atcttaggca
      781 agcaacccac tggatgtcgc taccagaacc gccgcaggag gtgaatcaat gacctggcct
      841 gaagcattca caacggtagg aattgcgatg gcggtggcgc tggtggtgta ttcgatttgc
      901 cgctggggat aaaaacggtt tgcgggaaaa ggatagttaa gtagaattgc agcgggtgct
      961 tgaggctatc tgcctcgggc atgaacacca acggcagata gagaaaagcc ccagttaaca
     1021 ttacgcgtcc tgcaagacgc ttaacattaa tctgaggctc aatccatgct gaacacatgt
     1081 aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg
     1141 atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg
     1201 aaagacctct gcgaggtgcg aatccgaacc ggtcagacgg aggtcgctgt cttcgtagac
     1261 tacgaatctg agaagtaaga gaccaggcgg gggagtaatc tcccgccacc tctgatgtgt
     1321 caggcatcct caacgcaccc gcgctttacc atactgaaaa tgctgtttga atgttcatct
     1381 ctgaaagagg actatgaatg aaaaaggtat tgattgcagc acttatttcc ggtgtgtctt
     1441 ttggcgcttt tgcacagcag ggtggtttcc aggggccaga agcagagcgt tcaacagtag
     1501 cgcaggcaaa agaactgaag gatgatgcat gggttatcct tgaagggagc atcgttaaaa
     1561 aagtgggtga tgaacgttat gagtttcgtg acaatagcgg gacaattgtc acggatattg
     1621 atgacagcgt atgggccggg cagaatgttt ctccgaaaga caaagtaaga attgagggtg
     1681 aaattgataa agacctgagc agtgttgaag ttgatgtaaa ggcactgaaa ttattaaagt
     1741 aaccgcccct gcttgttaag cccgtcttac tgacgggttt tctgtttgta cattccggcg
     1801 tattgcctta caattcgcgc agtcagcctg aacaactgac acctgctgtc accggagaat
     1861 ccgatgacac aacacataaa atcccacaat tctgaagccg acccggaaat taagcagggg
     1921 aggcgttttc gtgcgcctca gtatggctgg tttcactatc tgttctgtac gatcgatgag
     1981 gcagatatgc ttcaagaggc gtatctgcgt cgcggtgtcc gtgtggagcg gagtctgaac
     2041 gctgatcgtc tgacctggac cgtttctgta tatcttcctg ttcgtgcaca tctgccacgg
     2101 acacatgcct gctaccgtca gcgcgtctgg aggtaatgtg cgggtattac ttcgacctgt
     2161 tctggttccg gaactcgggc tggtggtcct taagcccggt cgtgaatcat tgccagtttt
     2221 tcatcgcggc agggtgctgg tggagccgga accgaaaaac atgcgggcgc tgccatctgg
     2281 agcggttcct gctgttcgcc agccgctggc ggaagataaa tcactgctgc catttttcag
     2341 cgatgagcgg gtgattcgtg cagctggcgg cgctggtgca ctgtctgact ggttattacg
     2401 tcacgtgaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa ccgttattca
     2461 cagttacggt gctggcgcaa tggtgttgtg ctggcactgc gacaaccagc tgcgcgacca
     2521 gacctccgaa tcacttgagc aacttactca acaaaatctg acagcctgga tgattgacgt
     2581 catacgccat gtaatgaatg gcacgcagga gcgggaatta tcgctggctg aattatcctg
     2641 gtgggcagtc tgcaatcagg tggtggacgc attacctgag gcagtatcgc gtcg
//
LOCUS       DAZFDP010000326         2693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFDP010000326 DAZFDP010000000
VERSION     DAZFDP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHJ1G_005313"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHJ1G_005313"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4766967.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHJ1G_005314"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHJ1G_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4766968.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          670 a          665 c          647 g          711 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttc
//
LOCUS       DAZFDP010000327         2681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFDP010000327 DAZFDP010000000
VERSION     DAZFDP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..63)
                     /locus_tag="ACHJ1G_005315"
     CDS             complement(<1..63)
                     /locus_tag="ACHJ1G_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766969.1"
                     /translation="MSALLSPLSLQAADVRRSGDE"
     gene            738..929
                     /locus_tag="ACHJ1G_005316"
     CDS             738..929
                     /locus_tag="ACHJ1G_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4766970.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(982..1215)
                     /locus_tag="ACHJ1G_005317"
     CDS             complement(982..1215)
                     /locus_tag="ACHJ1G_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766971.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1311..1934)
                     /locus_tag="ACHJ1G_005318"
     CDS             complement(1311..1934)
                     /locus_tag="ACHJ1G_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4766972.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(2023..2532)
                     /locus_tag="ACHJ1G_005319"
     CDS             complement(2023..2532)
                     /locus_tag="ACHJ1G_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766973.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          781 a          692 c          561 g          647 t
ORIGIN      
        1 ttcatctccg ctacgccgga catccgctgc ctgaagagat aaaggcgata ataaagcaga
       61 catcagtgtc agtgacagca aggacggaca gcgccggaat aagctttgtt cctcagtcct
      121 ctcgttcctg caacataatt ttccagtcaa acgaaaaacc ttttattcat gtcataccaa
      181 caatcaccag cctcctgacg atacctccca ccatgttttg cgatggcacg ctaccgccct
      241 tccggtattt acccgcatca accggtacca gaatgctgag taacaaacca gctagaattg
      301 acttaatttt gatcattata gaataatgat ctgaactaca gcaacatgac agatttatcg
      361 atcgatataa cacaaatgaa cagttcttta ttctgacaaa tatcgcaaaa acagcacatt
      421 acaaaattta taaccataat tactggaaaa gtgcagaaaa ttattccact gaatgctttt
      481 aatacagttg gttagcttaa aaaatagcaa tttttgattt cagtcattga aagttgcaat
      541 gtttgacgat tcaacagcat ttaagtgagt ttcaggatat gggaaggaca tgaaaggagg
      601 agcgcaataa aatacacagg aagaagggga aaatgtttcg taaaaacaga aaattaacag
      661 cgcacatcag gaaacgcagt ctcctgattc agtgctacca catgaaaatt tagttaactg
      721 ttaccgtact aaaatttatg tcagaagatg ttccattacc gaaagtcaat cagcgataca
      781 aagacgatca tggtgcgctt gtcaccctga ccagtgttga ggaaacccgg gtggtgttta
      841 tgcaggacgg ctatccgcat ccctgcatgc gaccgatgta caactttctg ggaaaattca
      901 aacccgaacc ccgggaagaa acagaataaa acgaaatccc tgccggcaat cacactacct
      961 gatagcagca taatccactt attaattctc atccccggag acaagcccag aaaaccactc
     1021 cgggtgttca ctgtatgccg tgttcagcgc atccctggaa acgtttatat aaaagctgtc
     1081 ctgcaatttg cgggtaacaa cttctgccag gcgattccgg ttatccacca gtccggcata
     1141 acctctctct gccagccact cattcagttc atgttccaga gacagattga taatatcata
     1201 atcggacctt gccattcccg tttccttagc acatgttttc aggcacgggg ataaagtata
     1261 aagaatgtca tactgctgct gccagagcaa tattccccct gatggttgta tcagagatgg
     1321 tatgccggta ctttgcgggt tgttcccgtc aggttatcgt gcacctccac ggtcgtggtc
     1381 accaccggca ttccggcctt tctcagcctc agaacatcag ccgcaatacg ctgactgcca
     1441 aaccagaaca ggccgtccag tgcagtcacc agtaaacccg cctccagtgc atacttcagc
     1501 cgttcacggg gcgctttcac ttcccgggca acctgctgat atggcaatga tgtgttttca
     1561 ttcccaacca cgcgacgaat acggtgagac agatgatacc ggtatgtatc cggcacacca
     1621 gaaaggctgg ccttcaggct gtacacgcag ccaaaccgtt tatcgttaaa caccacattt
     1681 ttctggctga tgccccattc ttcacgcagc gcggcaatca attgcggtgt acgggtaagc
     1741 agcaagcgaa aaggcagttc aaaactggtg acgtaatcca cattcagcag tgcaatacgc
     1801 aaccgttctt ctggcccggc ctctgtctgc cggcactcct ccagaacatc ctgccactgc
     1861 cggcgaagac ggggggattc gttccgttct gtaaaacagt atttatccgc cagatagtca
     1921 attcgtgtac gcatactgaa aggaattccg tataaagatt cagcagacaa aactttatca
     1981 gccagtaaaa attaacggaa gcattgatat tcctcccgac aatcaccgga tgattgttgc
     2041 aataacctgc gacataaagg actgaacagc cgtttttaac gccacgtatt gccctgatga
     2101 accaggccgg gccacccgaa aacatctttc cacccggcat tgtccgccag cacttatcac
     2161 cgaccaggtc tgttgcagta aatccaccag ttgcgcacat tcttcatcag ccatgacgcc
     2221 gactcgcgga tggcaaatac tcgtctggct gagcagcaac agacatcgta tctgtgctcc
     2281 ttcatgttgt tcccgcaacc agcgtaatac caccggcgct gatgggctga ccgtgtgatt
     2341 atgctccgta atacgttcgt gtacccgtcg gtgaaaggaa ctacaaaatg tctgaatctg
     2401 ttgaccatag attccttctg ccgaatagag cgtgaagtga aatgccaaca gatttggcca
     2461 gtggtccacc gcctgctgaa caaaacgccg gatttccccc ggctctgaaa ttaaggcttc
     2521 ggttatttgc acgaaatgtt ccttttcaac ccctgttgaa ttttgtcaag ccggtgcaga
     2581 cacagtaaag cgcgcatcaa cacctgaaag gtacgataca aacacaagtt acaaacaata
     2641 agaacggaag gacaattaat cagtgaggaa taataactaa a
//
LOCUS       DAZFDP010000328         2676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFDP010000328 DAZFDP010000000
VERSION     DAZFDP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(437..513)
                     /locus_tag="ACHJ1G_005320"
     tRNA            complement(437..513)
                     /locus_tag="ACHJ1G_005320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Arg,seq:tct)
     gene            complement(527..603)
                     /locus_tag="ACHJ1G_005321"
     tRNA            complement(527..603)
                     /locus_tag="ACHJ1G_005321"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(567..569),aa:Arg,seq:tcg)
     gene            complement(613..688)
                     /locus_tag="ACHJ1G_005322"
     tRNA            complement(613..688)
                     /locus_tag="ACHJ1G_005322"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Ile,seq:cat)
     gene            complement(729..842)
                     /locus_tag="ACHJ1G_005323"
                     /pseudo
     CDS             complement(729..842)
                     /locus_tag="ACHJ1G_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1191..1943)
                     /gene="ydfT"
                     /locus_tag="ACHJ1G_005324"
     CDS             complement(1191..1943)
                     /gene="ydfT"
                     /locus_tag="ACHJ1G_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4766974.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     ATLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            complement(1957..>2676)
                     /locus_tag="ACHJ1G_005325"
     CDS             complement(1957..>2676)
                     /locus_tag="ACHJ1G_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4766975.1"
                     /translation="GCQWPHSDWHSENMTTMRHAPGAIRLCWHCDNQLRDQFTERLES
                     MATDNCARWVLSVVRRDLGFDDSHVVTMPELCWWLIRNDLADALPESAARKALRLPKP
                     VVPSVTRESDLVPSVPATSIIQDKAKKVLVLKVDPESPESFMLRPKRRRWVNEKYTRW
                     VKTQPCACCGKPADDPHHLIGHGQCGMGTKAHDLFVLPLCRKHHDELHADTVAFEEKY
                     GSQLELIFRFIDRALAIGVLA"
BASE COUNT          692 a          726 c          589 g          669 t
ORIGIN      
        1 agaaatgaaa aaaggccacg cagttgcgca gcctgataaa ccctggttaa aatccacacg
       61 ataaaaatga caatgcaagt atctcatgct gttgcccgaa cctactcggg ctttttttgc
      121 atgtaaaaag gctcctgcga tgaggggcct ggatatatgc ctaatctctg tatacagcat
      181 gatgccgggt gcctcccggt gaattctgca atgaccagac agaatccgca acttgcctat
      241 acaatacgca accaaacatc tgtcattatg ccccgccgcc cagggggatt catcatgcag
      301 gattttttta acaaacgctc agcatgtcag gcaacagtcg actacctgaa ttgtgaggca
      361 tttaacattt cactgtccgg tgtctttcct gtaataaaaa gcccgcaaaa gagagtcagg
      421 gcagataagt gtggtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
      481 ggcaattgct ctgtccggct gagctaacaa cgcatgatgc tgataatgga ccgccatcgg
      541 ggacttgaac cccgcacagc cagcttcgaa ggctgacgct ctatcccgat gagctaatgg
      601 cggtatgtga tatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg
      661 ctcgctctca ccactgagct aaagggccgg gcgcaggata ataacggtac gtaactaatc
      721 ctgcaatatc atccgttctg actgactaaa tcctgaactt ccctgaccgt ctgctcaaaa
      781 cgttcagtct ccagctcaac gccaattgca cgacgcccca gcgacattgc tgctttgacg
      841 ctacagacat aaaaaagcca gccactgggg gaggctggca aactcgtaga gcaaaatgct
      901 gttacgcaaa cttcgttaca gggtcatcct gcaatacaaa aaatacacaa tatttagaaa
      961 actaatagtg ccatgtgcaa tttttaagat tttgttatta attgtggtcg caccttcctt
     1021 tctgtgtact ttccgtatag ctcacaggat tctgggtaca aaaaaacccg cgcatcggcg
     1081 ggttcttaaa tcttatcaac ggtagacata caaagcccat cgttgggaaa atcttatcca
     1141 tattttttga aaaatgcaag catcatgtcg tcatcttcgg cgaaaaccat ttatcttgtc
     1201 acctttctca attgtatctc tgcatatgct tcttcctgcc agcactttgt aaccagttta
     1261 tcaatgacat ctgcatatcc tttgtaccac tgataatccg tcaggtctgg taccagcttc
     1321 tggacatgaa gccgcgccag tgtggttggt aaacggctaa accggtttcc attgcaacgc
     1381 ccacaaacct tataaacagg cgtgccatga agccgggttc ttttttcatc caggacaata
     1441 cctttaccct tgcaccctct gcatgctgtg ctgacttctc ccttaccatg acaatgctga
     1501 catagttcct tcacccactc ttccttaata acagattccc cgcttctgga gtgtttcacc
     1561 acctcgcgca atacattatg aaatccagta ccagcacaat gctcacagcg agccttactt
     1621 gccgcagacc tggaataatc agcaaaggca aaattcacaa ggtaaggaat gatctgtagc
     1681 cgggtttctt cactcaattt attcaatgtc gggttatcca gtgccatcgc gtaattgagc
     1741 agaccttcaa tcgcaaactg aggatcctga acaccaactt ttgccaggaa taaagcaaac
     1801 ccaagcgttg cttttgactg caccatcccc tgcgcagcca tcacatccgt aattgttaaa
     1861 ccaccagagc ctgtcgccgg tgcgtcatcg ctcagttttg gagatttcgg ggagtaatat
     1921 ttcggtaagg cttcaaggtt catgctcgtt ctccacttac gccagtacgc caattgccag
     1981 cgcacgatcg ataaaacgaa atatcagctc cagctgagag ccatacttct cttcaaatgc
     2041 cacggtatcc gcatgcagct cgtcgtgatg ctttctgcac aaaggcaaca caaaaaggtc
     2101 atgcgctttt gtacccattc cacactgacc gtggcctatc aggtggtggg gatcatcagc
     2161 gggctttcca caacatgcac acggctgtgt cttaacccag cgcgtgtact tttcattaac
     2221 ccagcggcga cgttttgggc gtaacataaa agactccggc gactccggat ccactttcag
     2281 caccagcacc tttttcgctt tatcctggat gatgctggtg gcaggaaccg aaggcacaag
     2341 gtcactttcc cgggtgacag acggcacaac aggcttcggt aatctcagtg ccttacgggc
     2401 tgcactttcc ggtaaggcat ccgccagatc attacgaatc agccaccagc acagttccgg
     2461 cattgtcaca acgtgactgt catcaaaacc gagatcccga cgcacaacag acaacaccca
     2521 gcgggcacag ttatccgttg ccattgattc cagccgttcc gtgaactgat cgcgcagctg
     2581 gttatcgcag tgccagcaca gacggattgc gcccggagcg tgtcgcattg tggtcatgtt
     2641 ctcgctgtgc cagtcggaat gaggccactg gcagcc
//
LOCUS       DAZFDP010000329         2651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFDP010000329 DAZFDP010000000
VERSION     DAZFDP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..280
                     /locus_tag="ACHJ1G_005326"
     CDS             <1..280
                     /locus_tag="ACHJ1G_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4766976.1"
                     /translation="YVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSS
                     KKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(373..732)
                     /locus_tag="ACHJ1G_005327"
     CDS             complement(373..732)
                     /locus_tag="ACHJ1G_005327"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4766977.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1418..1708
                     /locus_tag="ACHJ1G_005328"
     CDS             1418..1708
                     /locus_tag="ACHJ1G_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4766978.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1732..1983
                     /locus_tag="ACHJ1G_005329"
     CDS             1732..1983
                     /locus_tag="ACHJ1G_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4766979.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2031..>2651)
                     /gene="traT"
                     /locus_tag="ACHJ1G_005330"
     CDS             complement(2031..>2651)
                     /gene="traT"
                     /locus_tag="ACHJ1G_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4766980.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          736 a          536 c          569 g          810 t
ORIGIN      
        1 atatgtctct gaccatggag aatctctggg agaaaatgga ttatacttac atggaacacc
       61 atataatgtt gctccagctg agcaaacgca tgtgcctctt attacctgga tgtctccggg
      121 gttcgtttcc tctaaaaaaa ttgatttgaa ttgtttgtca gaacatgctc taaacagaac
      181 agtaagtcat gataatatat tttcatcgtt actggggtta tggaatgtaa atacttcagt
      241 ttataataaa gaagatgata ttatatcctc ctgcagatag atactgacat ttttctgagg
      301 ataagaggtg aatacctctt atccttaagt aaatattaac caggtaactg tgaataattt
      361 agatgttggg cgttatgaaa gtaataaaac tccccatata atgatagcaa ctaaaatcgt
      421 aatcaacact gcggctgaac ccatatcttt tgctctgcct gataattcgt gatgttctga
      481 accaattctg tcaacaactg cttcaattgc actgttaatt atttcaacaa taattaccat
      541 cattgccgaa ccaatgagta atactcgagt aacagagtca acatccagcc aaaaggatat
      601 tatcagtgct gacagaacca gaatgcactc ctgacgaaag gctgcttcat agcgccatgc
      661 cgcacataag cctttccatg aataacccgc tgctttaata attctggtaa caccagtggt
      721 cgtattagcc ataaatacct cttatttaat tatatgtctg aatatgcatt ataaaatatc
      781 agttaacatt aacaggattt tgtaataagc cacatttatt ctttcgggaa ttaaaaaaca
      841 taacgaaatt ttgcctcatt ctgcatttca ttgaaatagt atgtttctca tcgaaagcaa
      901 gtttgtcaat ataaatttgt aacagaaatg actttccggg gataaacaaa actgttcatg
      961 gcaatggctt tctctgtcgc caacatggat acattgttcc ggataaagca ctgatgtcat
     1021 tgactgacgc agactatcag gccatctgat gctttctttt ccttcaccgg tattgatgat
     1081 atgccttatg aagggggact tattcctgta acccaatcac aggcactgtg attactggtt
     1141 tatgattgat gctgatttga gcagatatgc acagcagttg tggattcagg ggatattttc
     1201 tgccagatat tcataccaga ttgatgacct ctttgctcag atgctcttcg ttctgagcca
     1261 gccaggattt tgtggccaca tacattgttc gccgatgggg aatcgagaca ggcgattggc
     1321 tgtgatgcga ttttgccgct aactgaggat gagtccgccc ctcccgggga attcctgtaa
     1381 gttaagccgt acctgttgat tagcactaaa ctgggtaatg aatttatctg ccagtatgaa
     1441 tgatgccggg gacggttttg aactcctgat gtcgggtaat atgcaggatt ttaatatttg
     1501 ttgtcgtcgg gataatcatt atcctgaaca taaactgata cctttatcag agttatttga
     1561 cgttaattat ggactgaatc ttgagctgaa taaactggag aaagattctt caggaattaa
     1621 ttttgtttca agaacgtcca aaaataatgg cgtctccgcc agggttaaat taaaagatgg
     1681 tattagtcca tgccagcagc tgtgttaact gtagcagcag gaggttctgt tatggaatcg
     1741 tttgtacagg attcaccatt ttatagtgga agagatcttt attggttacg tcctaaagtt
     1801 gaactgactc tggaagagaa attgtactac tgctcttgta taagaagaaa cagacacaaa
     1861 tacagctacg ggcgtcaggc aaaccgaacg ctgaagaatt tactggtacc gtcattagat
     1921 tctgttccgg catgggtata tggtgtgaca gggaaaataa tatctgaatt gtctgaacgt
     1981 tgagactaag agggccggag gccaggtgaa tggccaccgg gatatttata tcagagtata
     2041 ttggcgattg attttgccag ctggtcttcc agatgaggtt tagcttcttc aaacttcaga
     2101 ttaacctggt tggcactgga tacaacccgg gtctggtatt tatgctggtt gccggtttct
     2161 gtgcttgtct gaattttgga gcctgatgtt ccctgacgca aagctgcaac attatccgtt
     2221 ctgacgctgg ttctggttcg ctcggcaatc tgaacatctg taatcattgt gtaatttata
     2281 tcttccacca ttgcatccgc ggccatgcca atcagaccgg tagccagtcc aacaccgaga
     2341 gtggtacctg ccgacccggt attgtacgct gtgattccac ctccaagagc agctccgact
     2401 gctgcacctt cataaccgcg actcagccat ccctgagatt cacgcagatc cattttgtct
     2461 gctttgagaa cgttagcctg gatccagtag taagctttat ccggggaagt catgatctga
     2521 taacctctgg ctttgactgc atcggcaatt tttccctgta gtccactcat gtctttatcc
     2581 gacgtgtttt tgatctgcag gaaaacggtg cgttcgctcg caggttcaag ccagatggtt
     2641 gaggtgtact g
//
LOCUS       DAZFDP010000330         2635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFDP010000330 DAZFDP010000000
VERSION     DAZFDP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="ACHJ1G_005331"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="ACHJ1G_005331"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4766981.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1185..>2635
                     /locus_tag="ACHJ1G_005332"
     CDS             1185..>2635
                     /locus_tag="ACHJ1G_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4766982.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENI"
BASE COUNT          841 a          564 c          542 g          688 t
ORIGIN      
        1 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
       61 tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc gagactatat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc gactggaaat
      241 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      301 ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      601 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      721 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      841 aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc
     1021 ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc aatatccact
     1081 aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct
     1141 caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga aaaataatta
     1201 ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac
     1261 agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa aaaccgtctc
     1321 ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca ggcggagatg
     1381 cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca
     1441 agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt
     1501 atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg
     1561 atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga
     1621 ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata
     1681 ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc
     1741 gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc
     1801 aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg
     1861 gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga
     1921 gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc
     1981 tttcttcaga cagagataag gcaataactc agttccatga gctattatca aaattacatc
     2041 acaaaccttt attagacagt aagagagaaa atatatcttc agatgaactc atatcattaa
     2101 caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt
     2161 tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag
     2221 aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa
     2281 caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact
     2341 ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc
     2401 tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt atagcacaca
     2461 aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc
     2521 cgttactaac aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg
     2581 tagataaagc agttgtacat cacctgatca ctccacaaaa aatagaaaat atata
//
LOCUS       DAZFDP010000331         2627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFDP010000331 DAZFDP010000000
VERSION     DAZFDP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1684
                     /gene="tssI"
                     /locus_tag="ACHJ1G_005333"
     CDS             <1..1684
                     /gene="tssI"
                     /locus_tag="ACHJ1G_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO4766983.1"
                     /translation="GVVTWFELGENDKNQMLYSMKVHPPLWRAGLRQNFRIFQNEDIK
                     SILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRMAAEEGIFFYEEHAYK
                     STDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSAQIRPSSVVTKDYTFK
                     RPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKNAHGQNFARWQMDGWRNNAETARGMS
                     RSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAVIP
                     ADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRFNWDRYNPADQDSSCW
                     IRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRPPGSLPGTKT
                     QMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKN
                     DQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGI
                     QAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1712..2173
                     /locus_tag="ACHJ1G_005334"
     CDS             1712..2173
                     /locus_tag="ACHJ1G_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4766984.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            2193..>2627
                     /locus_tag="ACHJ1G_005335"
     CDS             2193..>2627
                     /locus_tag="ACHJ1G_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO4766985.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNI"
BASE COUNT          662 a          693 c          790 g          482 t
ORIGIN      
        1 aggcgtggtg acctggtttg aactcgggga gaacgacaaa aaccagatgc tgtacagcat
       61 gaaggtgcac ccgccgctgt ggcgtgccgg tctgcgccag aacttccgta tcttccagaa
      121 cgaggacatc aaaagcatcc tcggcacgat gttgcaggaa aacggggtga ccgaatggag
      181 tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg gtgagactga
      241 ttacgatttt ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg aggagcatgc
      301 ttacaaaagt accgaccaga gcctggtgct gtgcgacaca gtccgccatc tgcccgaatc
      361 ttttgaaatc ccatggaacc cgaacacccg taccgaggtg agcaccctct gcatcagcca
      421 gttccgctac agcgcacaaa tccgcccttc ttccgtggtg accaaagact acacctttaa
      481 acgccccggc tgggccggac gttttgaaca ggaaggccag caccaggact accagcgcac
      541 gcagtatgaa gtgtatgact accccggacg tttcaagaac gcccacgggc agaactttgc
      601 ccgctggcag atggacggct ggcgaaacaa cgcagaaacc gcgcggggaa tgagccgctc
      661 gccggagata tggccgggac ggcgaattgt gctgacgggg catccgcagg cgaacctaaa
      721 ccgggaatgg caggtggtgg caagtgaact gcacggcgaa cagccacagg cggtgccagg
      781 acggcaggga gcggggacgg cgctggagaa ccattttgcg gtgatcccgg cagacagaac
      841 atggcgacca cagccgttgc tgaaaccgct ggtcgacggc ccgcagagcg ctgtcgtgac
      901 aggaccggca ggcgaggaaa tcttctgcga cgaacatggt cgcgtgcggg tgaggttcaa
      961 ctgggaccgt tataacccgg cagaccaaga cagttcgtgc tggatccgtg tggcacaggc
     1021 gtgggcaggc accggttttg gccacctggc gataccgcgt gtgggtcagg aggtgattgt
     1081 agacttcctc aacggcgatc cggaccagcc gatcattatg gggcgcacct accaccagga
     1141 aaaccgcccc cccggcagcc tgccgggaac aaagacgcag atgaccatcc gctccaaaac
     1201 gtacatgggc agcggattta atgagctgaa gtttgatgat gcgacaggga gagaacaggt
     1261 ctacatccac gcgcagaaga acatggatac cgaagtgctc aacgaccgta ccaccaccgt
     1321 aaaacacgat caccgcgaaa ccgtaaaaaa tgaccagacg gtcacgatcc aggaaggtaa
     1381 ccgccttctt acggtggaaa aaggccacaa gatcaccgga gtactgaaag ggtctttatc
     1441 tgaggatgtc tttcaggaca gaggcacgat tgccggttcg gtgcatgttg acgctgtaaa
     1501 caatggtggc gaaggcgacg gtatacaggc ttatacggcg attaaggaaa ttttgctggc
     1561 tgtggaggaa agcaaaattg cgctgacgcc ggatggcatt cagctacagg tcggggaatc
     1621 gacggtaatc aggctgtcga aggatggcat caccatcgtg ggcggttctg ttttcatcaa
     1681 ctgattcact cagattatca gggagtattt tatgcagttc acttttaatg aaggccacat
     1741 tcaactgccg tctcaatggc aggatcagtc aatgcaggtc ctggtgtcca cagataacag
     1801 tggcatcaac ctggtcatta cccgcgaagc cgttccacag ggaaccctga caccagagct
     1861 gtaccaggaa acgctggcgc tgtaccaggg caaacttgat gggtacaccg aacatgcctg
     1921 ccgggaaatc actctggcag aggccccggc ctggctgctg gattattcct ggaacggtcc
     1981 ggaagatgag gggaatcagg ggcggattag ccagatagcc gtattccagc ggcgtggaga
     2041 tacgctgctg accttcactt tcagcacctc cttatccctg aagaacagtc aaaagacgat
     2101 gctgctggag gtgataaaga gcttcacccc gttgccaccg gagaatgaca tccaaaagga
     2161 ccagccgcgc tgacataaac gagggataac acatgagtga aggaccaggc gggccacagg
     2221 gagcgaccgc aggcggtacg ctggcaatgc gaatgctgtc acagcaggcg atggtcgcca
     2281 gccagatgaa acgggcagcc aacgacaaag ccattgcaca gatgctggca gcaaagaagt
     2341 ccggcccacc tgccgccagg ctgggcgatg aaattcagca taaaagtttt ctgggggcac
     2401 tggcaggggc cgtgctgggg gcgatagtga ccatcgcaga aggttgcctg attatggccg
     2461 cctgcgccac cggcccttat gcgctggttc tggtgcctgc gctgatgtat gccagctata
     2521 aggcgagtga ttatgtggag gagaaacaga accagcttga atcatggata aacagctttt
     2581 gtgacacgga cggcgccatc aataccggtt ctgaaaatgt aaacatt
//
LOCUS       DAZFDP010000332         2598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFDP010000332 DAZFDP010000000
VERSION     DAZFDP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>2598)
                     /gene="eae"
                     /locus_tag="ACHJ1G_005336"
     CDS             complement(<1..>2598)
                     /gene="eae"
                     /locus_tag="ACHJ1G_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO4766986.1"
                     /translation="NAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESE
                     MMKAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTT
                     DDKALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVN
                     LQSGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGY
                     NVFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDI
                     RFNGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGID
                     YRHGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEY
                     KKQDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQS
                     AQDYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFT
                     ADKTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATV
                     TLKSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITY
                     TVRVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSG
                     VGTEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWK
                     SSNTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYF
                     DAENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVS
                     STYDLVTKN"
BASE COUNT          701 a          628 c          517 g          752 t
ORIGIN      
        1 ggttcttcgt aaccaaatca tatgtgcttg atacacctga tgactgttca gaagaggatt
       61 gtttaatcca agcagtcaat gatttagaac cagaatagta aggatattta tttgcagcac
      121 cccatgttga atagatattg gccaatagtt cttttgactg tgctaaattt gcgctatttg
      181 tcttacattt gttttcggca tcaaaatacg taactcgagt atttttatcc acagcaatta
      241 caatactacc cggtgcatta attgtgtatg tcgcactctg attatcacca gatactacag
      301 taattgtggc actccctttt tcatttaagg ttatcactcc cgagttatca acagaagcaa
      361 ttttagtatt actggatttc catgtgtatt ttccattgcc ccctgttgcc tgtagcttaa
      421 cttgaccata ctgtaaccag ttctttggca gagcccccgt gataccagta ccaattacgg
      481 tcactttatc accatcaata ctcaacgggg caaaaaactc aacggtagta gccttaactt
      541 ctgtacctac tccactcact tttgcactaa caatggcctt gccaggagta ttggatgata
      601 attttacagt agcataacca ttctgatcgg ttgttgcttc agtcttattc agggtcccaa
      661 aatccttaga aaaggtcact ttctgatcta ctacgggtgc cccctccttc atcactctga
      721 cagtataggt aatcgcatca gaaccatctg ccttcgctgt tgttttatca gccttaatct
      781 cagtaatact ggccttggtt tgatcaacaa atataaccgc gctggcatta agtggcgaag
      841 tcatctccgc ggttttagca gacacgacga cctgtcctgg cgtagccgac ttcagcgtta
      901 cggtcgcctt accgttacca tccgttctgg cactatttgc cccaagagtt gcagtcccgg
      961 atacaatact aaatgttaca gggacattag cctgagctac accattcttt ttaaccgtcg
     1021 cggtataggt aatagcttct atgccatccg ctttagccga tgttttatca gccgtaaagt
     1081 ccgttacccc aacctggtcc acaacctgcc cattcggtaa aacggtaata gtgagctgta
     1141 cattattaga actattacca tttcggtcat aggcgcgagc ggtcacttta taaatattgc
     1201 tgccgccttg cacataagca ggcaaaatag cctggtagtc ttgtgcgctt tggcttccgc
     1261 catgctgaat ctgaccgccc tgactgcgta atgcgctatc atcccagacg atacgatcca
     1321 gaccgtattt gctcttaacg atcaattgaa tcttctgcgt actgtgttca gtaccattaa
     1381 tatcatgcgg aatattcaga gaaagaatat cctgcttttt gtactccaga ataatattgt
     1441 tattacgctg aaccagatcg taacggctgc ccgataatgt tcttaactcg ttaacatact
     1501 gtggctcgat ttgctgagac cacggtttat caaactgata acggaactgc attgagtaaa
     1561 ggagatcatt ttcattaccc gtaccatgac ggtaatcgat ccccatcgtc accagaggaa
     1621 tcggagtgta gtttacacca acggtcgccg cgccaggatt cgactgcaac ttatcggaat
     1681 taaacaaagc aacattatca ccataatact gttcgtacat cagtttggcg cctaatgccg
     1741 gatatgatgg taaatagcca ttaaagcgga tatcaaaacc atttgccggg cgctcatcat
     1801 agtctttctt attgtatgac tcatgccagc cgctcatgcg gaaatagccg ttaacgctac
     1861 ttttgaaata gtctcgccag tattcgccac caatacctaa acgggtatta tcaccagaaa
     1921 aatcctgatc aatgaagacg ttatagccca acatattttc aggaaggaaa aaacgctggc
     1981 cagcacctaa atttgccgta aagcgggagt caatgtaacg cgcaccgacc tgaccaaatg
     2041 ccagcatgtt ttcggaatca tagaacggta ataagaagtc cagtgaacta ccgtcaaagt
     2101 tattaccact ctgcagatta acctctgccg ttccataatg ttgtaaccag gcctgcaact
     2161 gtgacgaagc ctggctgctg gccataccaa gagcggtatc tttcgcgtaa tcgccgttca
     2221 gtgagcgcga ctggagctgg ctaccaaggc tcgcggcctg ttgtgccgca taatttagag
     2281 ccttgtcatc ggtcgtgttg cttttagtcg cgtccgggga cattttagtc attttattcg
     2341 tatgaccagc gacaccacct gcagcaacaa caggtgccga acctaagaca ggtaaggcac
     2401 tatattcaac agacagtttt ttgagtggca aaatgatctg ctgaccaggt ccagccttca
     2461 tcatttcgct ttcggaactg tataaatgtt tattcagtga ccaaattacc gataaactga
     2521 taccctgtga tttagaaata ttggcaacag tttcacctgt ttttaacgta taaaaaaggc
     2581 gatcctgagc ggcatttt
//
LOCUS       DAZFDP010000333         2586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFDP010000333 DAZFDP010000000
VERSION     DAZFDP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(246..1433)
                     /locus_tag="ACHJ1G_005337"
     CDS             complement(246..1433)
                     /locus_tag="ACHJ1G_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO4766987.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1433..1798)
                     /locus_tag="ACHJ1G_005338"
     CDS             complement(1433..1798)
                     /locus_tag="ACHJ1G_005338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4766988.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            1880..1960
                     /locus_tag="ACHJ1G_005339"
                     /pseudo
     CDS             1880..1960
                     /locus_tag="ACHJ1G_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2199..>2586)
                     /locus_tag="ACHJ1G_005340"
     CDS             complement(2199..>2586)
                     /locus_tag="ACHJ1G_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4766989.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          590 a          758 c          624 g          614 t
ORIGIN      
        1 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
       61 cgataggaat ttaaatccca aaaagattaa aaaaacaccc aaaaacggat gtttattcaa
      121 cactgcctgc gtgccatgag tgcaccgccg tcaattaaat ggatcttatc gattgatact
      181 gcatatcact gatggttttt taccccattc ccctgaaaat ggacgcaact tctccgatgc
      241 ggctctcagc cacacacccg ctgttgcgcc agtggctcat gcatcaggac cagctctgtc
      301 agacggtagc cccgcttcag ccccgtaaaa cgcatctgac ctccgcacag gatgcacttc
      361 agcgggtcaa ccttcagtaa ccgctgatac atccctctcc acctgatttg catcgccgtc
      421 tttctcaccg tctccgttat gacgtacaca acatcttcca gtaaccggcg cttcaccgga
      481 ctcaggaaac cgtagtacct caccatacgg aaccccttat ccgccacatg ccaggagaac
      541 ctttccataa actcatcacc actcatcacc aggtattctt cccgttttgt ccggtgactg
      601 ttgtaacgca gaccaatttc atcctgacca gcatagtgct ccagacggct catcggcacc
      661 ggcggttttt tcaggtaaga gccgaagtac accgccacat gtgtggcgtt atccatcacc
      721 cgggatacgt tgacattcca gccccgccgg taatgactgt ccaggaagcg attccagtcc
      781 cgtttactgc ttccttctgc tgccagcgca tccggtatca ccaggtccgg atatttccgt
      841 gacagtaacc gcgttatccg gtaacgccac atactcatca ccttacgggc gtaaaaatga
      901 aggtttttcc aggtgtggtc tgatgtcacg ccgccggtcg ttgtcgacag gtgaatgtgc
      961 ggatgccact cctggtcacg tccccatgtg tggatcaccg tgaatatccc cggcaccaca
     1021 tctgcctggc ggcagatttc ctgtatcaca tccgcagcaa tgcggctcat ctctgccagt
     1081 aacctccggt tgtggaacac cagggaccag tactggcagg gaagtgtgaa cacaatatgc
     1141 tgccacggac agtcgggaac cagactcagc agatactgta tccactgtgc gccagccttc
     1201 actccgcagt gcgggcagga gcgactttta caccggaagc agaccttttt tatgtggctg
     1261 cagtccggag atgaacagca ccactgtgtg taccccatca gcgtggtccc gcaggccatg
     1321 atttttgtca cagactcaat gactaccgga cgcactgaac cttccggttg tttctccagc
     1381 cagttaagcc agcggtttcc ctgctgaaaa atgtcggcaa aacggggaag catcagaagg
     1441 gcggggggac tccgtccggc cagtgaaccg tgccacactc cgggcagtac atgccgccgg
     1501 cgctgatacc ggcaagaatg gtcgcaaact cccgctccgt gcagcgggct atttcaggat
     1561 acccttcgtc atcaacacgt acaaaccaga agaccagctt tttgtttccg gcatccacaa
     1621 agaagggaat attcaggtct gcgcagcact caacggcatc gtcaaaatta tcaaagcgca
     1681 gagcttctgc gtcttcttcg tcaaagaagt catcgtcgtg aagcttcacg acatagcggg
     1741 gaagtttgct tctttgaggg gcaggttttc gtttacgggg tttagctgaa cgggacatat
     1801 aactacctga aagacaatga tattgtctgt ttttataacg gtaatttcag gccatgacaa
     1861 gccgcagccg ccaggctgcc tactcggcgt catagcagac tggccggtca accgggtcag
     1921 cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc
     1981 cctcgctgta cgcttacgtt tatattcagt ttcctgcagt gctgcctgtg gctgagctgc
     2041 catctgcctg tcccttacgt gagtcacccc gtaacctgat gctgaggcat tgctcccttc
     2101 ataaaacatg gtttactcac tacagcttat ctacctgctc cagcttatgt tatgtctgtt
     2161 ctgctgacca ctgcttatat aaggaagagc gtatgctgtc acttcagggg aggcagtgta
     2221 cgcaggatct ctgcagcatc gcgcccgtca cctgtgaaag gcactaccag cgtggcagcc
     2281 atatccagcg caaaaactct ggtataaacc tccatcgaac gtggatccct gtgaccagcc
     2341 agtgcctgga tgactttccg gggctggcgg tgatagagca tgtgcatgat atagctgtgc
     2401 cggaaggtgt gtggtgtgac cggaatcgaa aagtgtaccc cgtcagcttc ggcccgtctg
     2461 acagcctgct tcagccagtt gcgcatggtt tcgtcagtca cggcccataa tggttcacga
     2521 cgacggggcc gggtggtgat catccagctt tccatctgcc tgacatagct tatatctgtc
     2581 agcgga
//
LOCUS       DAZFDP010000334         2582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFDP010000334 DAZFDP010000000
VERSION     DAZFDP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1289
                     /locus_tag="ACHJ1G_005341"
     CDS             <1..1289
                     /locus_tag="ACHJ1G_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4766990.1"
                     /translation="TPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETP
                     FGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEE
                     IEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERP
                     DAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGR
                     HDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPI
                     KGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPN
                     NPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            1286..1492
                     /locus_tag="ACHJ1G_005342"
     CDS             1286..1492
                     /locus_tag="ACHJ1G_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4766991.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1489..>2582
                     /locus_tag="ACHJ1G_005343"
     CDS             1489..>2582
                     /locus_tag="ACHJ1G_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4766992.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAG"
BASE COUNT          612 a          665 c          786 g          519 t
ORIGIN      
        1 ccacgcccaa agtgagaggc acctgccaga ttgagcgtgc agccagtgaa tccccgcatt
       61 ttatgcgttt tcatgttgcc tgcccgcact gcggggagga gcagtatctt aaatttggcg
      121 acaaagagac gccgtttggc ctcaaatgga cgccggatga cccctccagc gtgttttatc
      181 tctgcgagca taatgcctgc gtcatccgcc agcaggagct ggactttact gatgcccgtt
      241 atatctgcga aaagaccggg atctggaccc gtgatggcat tctctggttt tcgtcatccg
      301 gtgaagagat tgaaccgcct gacagtgtga cctttcacat ctggacggcg tacagcccgt
      361 tcaccacctg ggtgcagatt gtcaaagact ggatgaagac gaaaggggat acgggaaaac
      421 gtaaaacctt cgtgaacacc acgctcggtg agacgtggga agcgaaaatc ggcgaacgtc
      481 cggatgctga agtgatggca gagcggaaag agtattattc agcgcccgtt cctgatcgtg
      541 tggcttacct gaccgccggt atcgactccc agctggaccg ctacgaaatg cgcgtatggg
      601 gatgggggcc gggtgaggaa agctggctga ttgaccggca gattattatg ggccgtcacg
      661 acgatgaaca gacgctgctg cgtgtggatg aggccatcaa taaaacctat acccgccgga
      721 atggtgcaga aatgtcggta tcccgtatct gctgggatac tggcgggatt gacccgacca
      781 ttgtgtatga acgctcgaaa aaacatgggc tgttccgggt gatccccatt aaaggggcat
      841 ccgtctacgg aaagccggtg gccagcatgc cacgtaagcg aaacaaaaac ggggtttacc
      901 ttaccgaaat cggtacggat accgcgaaag agcagattta taaccgcttc acactgacgc
      961 cggaagggga tgaaccgctt cccggtgccg ttcacttccc gaataacccg gatatttttg
     1021 atcttaccga agcgcagcaa ctgactgctg aagagcaggt cgaaaaatgg gtggatggca
     1081 ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga ggcgctcgac tgcttcgttt
     1141 atgcgctggc ggcgctgcgc atcagtattt cccgctggca gctggatctc agtgcactgc
     1201 tggcgagcct gcaggaagag gatggtgcag caaccaacaa gaaaacactg gcagaatacg
     1261 cccgtgcctt atccggagag gatgaatgac gcgacaggaa gaacttgccg ctgcccgtgc
     1321 ggcactgcat gacctgatga caggaaaacg ggtggcaacg gtacagaaag acggacggcg
     1381 agtggagttt acgaccactt ccgtgtctga cctgaaaaaa tacattgctg agctggaagt
     1441 gcagaccggc atgacacagc gacgcagggg accagcagga ttttatgtat gaaaatgtcc
     1501 accattccca cccttctggg gccggacggc atgacatcgc tgcgtgaata tgccggttat
     1561 cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt ggaacccacc gggtgaaagt
     1621 gtggatgcag ccctgctgcc caactttacc cgtggcaatg cccgcgcaga cgatctggta
     1681 cgcaataacg gctatgccgc caacgccatc cagttgcatc aggatcatat cgtcgggtct
     1741 tttttccgac tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc
     1801 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgaaga tgactgttgc
     1861 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
     1921 cacgccttta acggtgaact gttcgttcag gccacctggg atacccgtcc ctcgcgactg
     1981 ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc
     2041 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat
     2101 tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgc
     2161 gagttacccg gcggtcgtgc ttcgttcatt cacgtctttg aacccgtgga ggacgggcag
     2221 acccgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
     2281 cagaacacgc agctgcagag cgccattgtg aaggcgatgt atgccgccac cattgagagt
     2341 gagctggata cgcagtcagc gatggatttt attctgggcg cgaacagtca ggagcagcgg
     2401 gaaaggctga ccggctggat tggtgaaatt gccgcgtatt actccgcagc accggtccgt
     2461 ctgggaggcg caaaagtgcc gcacctgatg ccgggggact cactgaacct gcagacggct
     2521 caggacacgg ataacggcta ctccgtgttt gagcagtcac tgctgcggta tatcgctgcc
     2581 gg
//
LOCUS       DAZFDP010000335         2575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFDP010000335 DAZFDP010000000
VERSION     DAZFDP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..101)
                     /locus_tag="ACHJ1G_005344"
                     /pseudo
     CDS             complement(9..101)
                     /locus_tag="ACHJ1G_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(134..472)
                     /locus_tag="ACHJ1G_005345"
     CDS             complement(134..472)
                     /locus_tag="ACHJ1G_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4766993.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(475..780)
                     /locus_tag="ACHJ1G_005346"
     CDS             complement(475..780)
                     /locus_tag="ACHJ1G_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO4766994.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1095..1745)
                     /locus_tag="ACHJ1G_005347"
     CDS             complement(1095..1745)
                     /locus_tag="ACHJ1G_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4766995.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            1826..2011
                     /locus_tag="ACHJ1G_005348"
     CDS             1826..2011
                     /locus_tag="ACHJ1G_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4766996.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2121..2417
                     /locus_tag="ACHJ1G_005349"
     CDS             2121..2417
                     /locus_tag="ACHJ1G_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4766997.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVVAGDSKQIDMQF"
     gene            2450..>2575
                     /locus_tag="ACHJ1G_005350"
     CDS             2450..>2575
                     /locus_tag="ACHJ1G_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4766998.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSG"
BASE COUNT          672 a          578 c          536 g          789 t
ORIGIN      
        1 atctcacctc aaataagtgg tttgctgcct aatttcattt tctggcgacc aacacaagtc
       61 acacccattt cactgcgtgg cttgctgtaa taaattaggt tagttcagcc aataaaaaac
      121 ccaccgaagt gggctatgac cattttttat ttggatttcg ttggtgagcg tgattaacaa
      181 ctctgtgcat tacatcctca tatttttcat cttcaatttt ttcgacatcg cgaggaaatg
      241 gtgttgctaa tgctttgtca actttgtcca ttgggtcttc attaatctta tattcaggac
      301 cgtcatctat agcattaaat ccaggtgtta caccgttttt taatgcatat gctatcctct
      361 tttcccatct cgctattctc ctcctgtctc gagatgtaag acctctatca gatacttttc
      421 tgttttgtcc gcggtcagga ttaacataaa tagtcttttt caccataagc atactcaata
      481 agcaccgtac ggtagtttac tgtacaattt tattttttgg actgcatgta ttttgtttcc
      541 taatgggttt gaatccttgt aataaatact tctatttttt cgaacaactt cttctttctt
      601 cttgcagcaa aggcttccta gtgatgctgc tttgtctgct ctgacgcaac cagagagctt
      661 tagcgcaatt tttcgcgcca gtgcttcatt actgcgtcgc tcggcaataa gttctgctct
      721 gcgagctttg tagcggcttt ttgccgtacc tttggattct ttccagacaa tggttaccat
      781 gatggtctcc tttaagtggc tttggcgcat gacgcgtcga ggtgcttatc ttctcgatcg
      841 ctgtcttgca gctgcaattc gcgccatccc caaaaccact caagttctgg tctcaacggt
      901 taggttgaga gttcgtcgat gttaaagagc ctgccaatct gttccgtttg gcttccagcc
      961 tcctgctgac ggcttaaaat taagacctct taattttttg gtcaagtgca tttttgaaga
     1021 aaacttaatt ttatgggcgc gaatttagtt tgtctttgat ttttaacggg aaataaaaaa
     1081 ggggcgaaag ccccttaagg aaggtttgct agcttggcat caacgacaac gccaatgatt
     1141 ttacagttcc cattgatttc aatcattggg tattgtggat tgagtggttt caggaatttt
     1201 ctaccggcat caataactaa ctttttgaat gtcgcctcgt tttctccttc aagtttggcg
     1261 actaccagct ttccattacg tggttcgact tctgggtcga cgagaataat catcccttca
     1321 ggaatactca gtcctgccgg ggcagtcatt gaatcacctt taacgtcgag ccaaaaagag
     1381 tcttcagaac aatctaccgt tgtgtcgtac cagttatcta ttgcacgcct atgatatggc
     1441 tctacagctt ccatccaaca tcctgcgctt acccaactaa ttagaggata cgaacctctt
     1501 ggatcatgcc tgctgtgata ggcaatgttt gaaagactat cctctccttt caacaggtaa
     1561 tcaggggagc actgcaaagc cttggctaag gccaataggt tttcgccatt gggctcagtt
     1621 tcagatcgct cccattggga aatagcaaca ttagacacgc caaccatctt gccaagggca
     1681 gcctgcctaa tcttgagttc ttttctgcga gcgcgaatac gctcacccat cagttgtgta
     1741 ttcatagtta agacatctta aataaacttg acttaagatt cctttggtgg ataatttaag
     1801 tgttctttaa tttcggagcg agtctatgta caaaaaagat gttattgacc acttcggaac
     1861 ccagcgtgct gttgctaaag cactaggcat tagcgatgca gcagtctctc agtggaaaga
     1921 agttatccca gagaaagacg cctatcgatt ggaaatcgtt acagctggcg ccctgaagta
     1981 tcaagaaagt gcttaccgcc aagcggcata agtaaattgc tctttaacag ttctggcctt
     2041 tcacctctaa ccgggtgagc aaacatcagc ggcaaatcca ctgggtgtgc cgctatatat
     2101 caatatagga aaattaacaa atggcacaag caagttacag caagctaaca cagcgagaaa
     2161 ttgatcgcgc agaaacagat ttactcatca acctgtcaac gcttacccag cgcggtctgg
     2221 caaagatgat tggctgtcat gaatcgaaga taagcagaac ggactggaga tttattgctt
     2281 cggtcttgtg tgctttcgga atggcatcag acatcagtcc gattagcagg gcttttaagt
     2341 atgcgcttga tgaaatcaca aagaaaaaat ccccggtggt cgccggggac tctaagcaaa
     2401 ttgatatgca attctgaggg aattactgga tcaatccaca ggagtcatta tgacaaatac
     2461 agcaaaaata ctcaacttcg gcagaggtaa ctttgccgaa caggagcgta atgtggcaga
     2521 tctcgatgat ggttacgcca gactatcaaa tatgctgatt gaggcttatt caggc
//
LOCUS       DAZFDP010000336         2509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFDP010000336 DAZFDP010000000
VERSION     DAZFDP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1506)
                     /locus_tag="ACHJ1G_005351"
                     /pseudo
     CDS             complement(1..1506)
                     /locus_tag="ACHJ1G_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1526..1873)
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005352"
     CDS             complement(1526..1873)
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4766999.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(1870..>2509)
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_005353"
     CDS             complement(1870..>2509)
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4767000.1"
                     /translation="RSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFIASGI
                     AWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQALQP
                     GAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNP
                     FWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
BASE COUNT          493 a          704 c          683 g          629 t
ORIGIN      
        1 ggttggcagg ccaggtgttg attacactga tcacgtggcg tagccacgtt tccggctcga
       61 ttccgttaag tttgcagcta ccgatcaggc tgtacatcac tgccgcactc tcgcctccac
      121 catcagagcc gaagaacatg tagttacgcc gccccagtgc aactacccgg agggcgtttt
      181 cacacaggtt attgtcgatc tccacccagc cattgcggca gtattcgttc agagcgtccc
      241 attgcttcag cagataggtg aacgctttcg ctgtatccga gtggcgcgac agcgtgctca
      301 tctgcccctg gagccactca tacaacgact gcattagcgg taccgttctg gcttttctga
      361 ccgccagtcg ctcttctgcc ggactgccgc ggatctcagc ctcgatagcg tacagttccc
      421 cgatacgacg gagcgcttct cccgttactg tcgttggatg gcggacatgt acatcgtgga
      481 tcttgcgtcg ggcatgtgcc atacaagccg cttcggttac ctgaccgctt tcgtaaagag
      541 cattgtaacc cgcatatgca tcggcctgca ggatacctct gtagtccgcc agatgttgct
      601 gtgggtggat gcctttgcgg tcgggagagt atgagaacca caccgctgcc ggcatggtcg
      661 aaccggcgtt gcgatcgtca cgaacataga cccacagccg tccggtacgg gttttaccct
      721 gacccggctc cagtacattt accggcgtgt catcggcatg cactttaccc gccatcagca
      781 catagtgctt cagttcatca tacagcgggc gaagctgctc tcccatgatg tcaacccagc
      841 gccccatcgt attgcggtgc agctccacgc cctggcgggc atagatttcc gactgacggt
      901 acagcggcag atgctcggcg aacttagcca tgattatgcg ggccagcaga gccggactgg
      961 cgtaactgcg ctcgatgggt tttggtggct gcggagcctg aactatacag tcgcaccggc
     1021 tgcaggccag ttttgggcga accgtttcga ttaccctgaa cgcggtgttg atgatatcca
     1081 gttgttcaga gatgctttct cccagcggtt tcagtttgcc gctgcagacg gggcattcgg
     1141 tttctgccgg ggagataacc tgcctgtcac ggggaagtgt tgccggaagt gctttgcgga
     1201 cgggagagtc tgatgttttc ggcgctgtct ctccggccat tgaggtgagt tgcaactgcg
     1261 cctcaccaag cctgttctgg agctcggtta tacgcgtttc tgcccgtgcg atcttctttt
     1321 ctatcttctc gcggcttttc tcgctgctgc gaccgaacaa cattctctgc agtttagcga
     1381 ccagcgctct gagtgagctg atctcgcggg catagccggt tatttcacca gacagacgat
     1441 aacagcctgc tgtgcgatca gcagggcctt cagttgctcg atgtcgtcgg ggagtgtgtt
     1501 gttcattccc ctgttttatc acgggttata tccggatgcc aggccgttct gtccgtttgg
     1561 gatgttgcca cgcgatcccc tccagtagca tggataactg agctggcgtc aggtgcactt
     1621 tcccttcccg ggttaccggc cagacgaagc ggccccgttc caggcgtttg gcgaacaggc
     1681 ataacccgtc acgatcggcc cacagtattt tcaccatttt gccactgcgg ccccggaaga
     1741 cgaagatatg cccggagaac gggtcatctt tcagcgtgtt ctgcaccttc gaagccaggc
     1801 cgttgaagcc acaacgcata tctgtgatgc cagcgatgat ccagattctg gtaccggttg
     1861 gcagcgttat catcgggtac ctcctttcat ttcgcggatt agcgcccgta acatttccgg
     1921 agtgagaggg tcaaacagtt ttaccacacc tgatttaaga tgcagctcgc accgtgggac
     1981 gtttccggga tcactctcag ggcactcatc aggcttgtta cgccagaagg gatttgtaac
     2041 tggtctggtc ggctctggcg tatcagtcag agccaccggg acaggcatgc attcctgtat
     2101 gtcatcatcg ctcagtaagc cgtcctcgta ctggcttttc catttaaaca gcaggttatc
     2161 attgataccg tgctctctgg cgatccgggc aacaacagca ccgggctgta atgcctgctt
     2221 agccagacgg accttaaatt cacggctgta gctggctcgc cgttcttttc gccatgtgcc
     2281 ttcgctgatt tgaggctctg ttaattcctt ctttctgttg gcataaagga tggcatcaag
     2341 ctgagctaat gaaactgaat cgggcaatgg ccatgcgata ccggatgcaa taaatcgctg
     2401 aaaaagcgta tgtattgtgg aatgactgag acctagacgc tgagcgatgg cccggatggt
     2461 cagtttatct tcaaatctta aacgcagagc atcaggcaaa taagaacgg
//
LOCUS       DAZFDP010000337         2508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFDP010000337 DAZFDP010000000
VERSION     DAZFDP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1379)
                     /gene="emrY"
                     /locus_tag="ACHJ1G_005354"
     CDS             complement(<1..1379)
                     /gene="emrY"
                     /locus_tag="ACHJ1G_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4767001.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGS"
     gene            complement(1379..>2508)
                     /gene="emrK"
                     /locus_tag="ACHJ1G_005355"
     CDS             complement(1379..>2508)
                     /gene="emrK"
                     /locus_tag="ACHJ1G_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4767002.1"
                     /translation="RRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADP
                     ISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQ
                     DKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQ
                     AYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETV
                     SPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMG
                     TGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIA
                     EMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT          750 a          555 c          504 g          699 t
ORIGIN      
        1 gaaccatagt atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg
       61 atggttgctg tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc
      121 aacgacgtac caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg
      181 gcaaatttat tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag
      241 gcaacggcga atccctggaa aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc
      301 ataaatgtca cagaacgcca gtaatagcaa accgcataca tcaaaaaact aaatgtcact
      361 aacaaccgca tgtctatttt gttgccataa cgtcctatca aaggtgaaat taatagtggc
      421 atgatgccga tgggcgcata agcaagtccg gcccatatcg cattataccc catcgtttcc
      481 tggagtaact gcggcataag gacgatcgct ccagagtaaa ataaatacgc gcatgtgata
      541 ctcacaatac caatggtgaa gttacgggac ttaaacaaac tgagatcaag aatcgggttc
      601 tctgaggtcg actcccaaat gactaaagag atcagagaaa taactgatac tactgttaat
      661 attattattg tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cataatttgc
      721 aagccaccaa caccgagcac taacagggtc agtcctggta gattcatttt gaccggtgaa
      781 gtttcagttt ctcttccttt aagtaaggtt aagcataatg tcaggacgat aatccccata
      841 gggacattga ttaaaaatat ccaaccccag ctaaagttat cgcaaatata accgcccaat
      901 atcggcccac atatcggagc gataatcacg gtcattgacc ataatgccag agcaaatgtt
      961 cttttttctg gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc
     1021 gccattaatc cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta
     1081 cacattaagg aagacagcga aaaaaaagtg actgaaagta aaaataacct taattcgcct
     1141 attctttgtg ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgag
     1201 gtgataaccc aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt
     1261 gcgacgttag aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata
     1321 gtaacgcacc ataacgtccc accggttaat ggtgccggag tcgatttagt gattgccatt
     1381 aaagttgtcc attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga
     1441 taactaaagc cttactggta taagccggca tggacgtcac ggttgaagcc agatcaggca
     1501 tctcagcaat gtcttcgttc ttcgtatcaa tagttgcagt catcgataaa ccaatgcgta
     1561 aggggtgttc catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga
     1621 ttttgatcca gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc
     1681 ccatattgat ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga
     1741 taatattgac tgattgacca atccgtacat ccgtgagttg tgtttcttta aagttggcat
     1801 taacccacat ttgacgtgcc ggtacgacag ccattaacga ttgtccggga ctcactgttt
     1861 cgccgacctg aacactccgc tgggcaatat agccagtaac cggactcctt atatccgtac
     1921 gtttaagcgc caaccaggct tctttagttg catccgccgc ttccacgact tgtggctgac
     1981 ggtttaatgg tgtgttcatt actaaagctt tattcgcttt ataagcctgg atagcggcat
     2041 tcagtgccgc tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa
     2101 taaccccctg cttcgctaac ggtactcgac ggttataatc ttctaaagat tgttgatact
     2161 gaatacgtgc tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg
     2221 tttgccgaac aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag
     2281 ttttatccag tgaaactaaa atgtcacctt gtcgaacgta gttcgtatct ttatgattaa
     2341 cgacagtgac actacctgag acttgtgcag aaattggatc tgcattcccc gtgacatagg
     2401 cgtcatctgt actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa
     2461 acgcaataaa taaaactact gccaataaag aaaagtattt tcttctgt
//
LOCUS       DAZFDP010000338         2477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFDP010000338 DAZFDP010000000
VERSION     DAZFDP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..253)
                     /gene="escO"
                     /locus_tag="ACHJ1G_005356"
     CDS             complement(<1..253)
                     /gene="escO"
                     /locus_tag="ACHJ1G_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO4767003.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKE"
     gene            complement(313..1653)
                     /gene="escN"
                     /locus_tag="ACHJ1G_005357"
     CDS             complement(313..1653)
                     /gene="escN"
                     /locus_tag="ACHJ1G_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO4767004.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1637..>2477)
                     /gene="escV"
                     /locus_tag="ACHJ1G_005358"
     CDS             complement(1637..>2477)
                     /gene="escV"
                     /locus_tag="ACHJ1G_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4767005.1"
                     /translation="DSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSAN
                     SIVYKTNEANQQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIME
                     RKYNELVKELQRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCE
                     YVRIALRRHILGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQII
                     GFLKNIVNPTGNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVND
                     FRA"
BASE COUNT          698 a          592 c          463 g          724 t
ORIGIN      
        1 cctcttttgt tcgtttttgt gaatcgagca atgaacgctt ttctttagaa agtgaggatt
       61 ttttctcata taatcgctgc ttttgctcat caaatgcatt gtttttaatc tggtatttat
      121 acaacgacac tttatccaga ttcaccagcg atgcacaata cgcctgctta gaggcaatcg
      181 actctttaat ggcttgctcg cagtcatcga gtttaccatc cacctctttt atctgagagt
      241 taatcttagc cattgattct ctcaatcgat tcgctctgct tttacgaata gataaaattc
      301 tgtccaacat attcaggcaa ccactttgaa taggctttca atcgtttttt cgtaactact
      361 gatatctttg gtgctttgtt gtataaagct ctgaattgct ttcctgtttt ttattgcctt
      421 atccgcttca ggatcttgcc ccatcgtgta ctcaccaata cgaataagca gttcaatatt
      481 cttataggtt gcaataagct ttttgcattc agccgctgca cgtaaatgtt ccgatttaac
      541 aacgttatgc ataacacgac tggcgcttaa accaatatca attgcaggga aatggttttc
      601 ctctgcaagt tctcgggtaa gcacaatatg tccatcaaga atagagcgaa cttcatcacc
      661 gatgggatca ttcacattat cgctttctaa cagcaccgta taaatcgcag ttattgaacc
      721 ttttggcgca ggtcccgcac gctctaataa tttgggtaac gaggaaaaaa cactcggagg
      781 gaatccccct cttacatcag gctctcccga tgcaagccca acatcacgcg cggctcgggc
      841 ataacgagtt acggaatcca tcattaataa tacatttttg ccctgatccc ggaagaactc
      901 tgcaatggtt gtcgccgtaa atgcggcctt cattctttcc agcgcgggac ggtctgacgt
      961 tgtgacgacc agtacacatt tagaaagcgt ggattgaggt aaaagcgcga ggaattcatt
     1021 tacttcgcga ccacgctcgc cgataagggc aaggacaata atatccgcgg atgcgccatt
     1081 acaaatcatt cccaaaagcg tacttttacc aacgccggac cccgcgaaaa taccgatgcg
     1141 ttgcccaata ccgcaagtaa gtaaaccatc tattgccctg acgccaagcg taaatggctg
     1201 atcaataacc tgccttaata aaggatcggg gggttcagca tacaaactgc gttcaaacgg
     1261 aagataaggt gcagtaatat tgctccccat tggtctgcct ataccatcaa ccagtcgccc
     1321 aaggagttcg tccccaacac gaatcttgaa ctcttccccc tgataactta gccactggcc
     1381 acaatacatg ccggaaatat gctcaaaagg cagcaggaaa acctcatctt catcaatagc
     1441 gatcacctca gctaaacgtt gactgggttc tatcttgtaa aaggcaccaa tacgcgcttt
     1501 tggtaaacgc gccttgataa tcgtcccgcc gatattggta atcttgcctt catatctggt
     1561 agacgaattt aatgataatg tcggcacagt gctattgagc actttctgga tacgtgggta
     1621 tctttccaat acagaatcat gctctaaaat catttaccgt tcctaatact ttaagttcga
     1681 tattattccc aacttcctga aaagagagca cggggactga cgggaacgaa ccttcaatca
     1741 ttttcttcac atagcgcctg atatctaaag cggtcagaat gacgccattc cccgttggat
     1801 tcacaatatt ttttaagaag ccaataatct gctcacttcg ctccggtgag atattcaaat
     1861 acgaacctga tgacgtttgt ctgatcgact ctcgtaactc attttcaata tcagagccaa
     1921 taagccagac attcagaagt gtaccgcgaa cgctatagcg acctaaaata tgccgacgga
     1981 gggcgatacg aacgtattcg cacaagataa ccacatcttt ttcttttgtg gaccaaaaaa
     2041 taagcgtttc aaaaatagtt cgtagatctc taattgagat attttcctct acgagacgct
     2101 gaagaatatc cacaatcttg ctcagaccaa gctgacgctg aagctctttc acaagttcgt
     2161 tatatttcct ctccatgatg tccatcagat aacgcgtttc ttgtacgccg atgaactctt
     2221 tggcattaga caatacaagt ttctttagca aaaacgttat cttgtcattt gtttttgcat
     2281 aaacatccat acctgagacg tgagctagtt gttggttagc ctcattcgtt ttatacacga
     2341 tagagttagc cgaaagcttt tcctggacaa attcaaatga tatctctgca tgcccagcct
     2401 caaaataaac agtatcatct gtaagagtat ctttgtatat tgactcttga tagagcaaaa
     2461 attctatagc gctatca
//
LOCUS       DAZFDP010000339         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFDP010000339 DAZFDP010000000
VERSION     DAZFDP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1983
                     /locus_tag="ACHJ1G_005359"
     CDS             <1..1983
                     /locus_tag="ACHJ1G_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4767006.1"
                     /translation="DAASRTLTLDREVTLPETGTPTVNLINGSGKPVSVAITAHPAPD
                     RIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVD
                     NGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSG
                     SGENSRLLTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPA
                     GVELTPGYFQITAVPKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVSG
                     PHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWEL
                     IDNSQLADEMAEMKTSITETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLAALYM
                     LKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQ
                     LFMNDVFLKRLFAVSITSSGNPPAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINE
                     NCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFR
                     GGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTL
                     TFTVSSSGANNWTPTTSISDLLVVVMKKATAGISIS"
     gene            2051..>2430
                     /locus_tag="ACHJ1G_005360"
     CDS             2051..>2430
                     /locus_tag="ACHJ1G_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4767007.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTHYSDTRWHEDFVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT          590 a          629 c          715 g          496 t
ORIGIN      
        1 gatgccgcca gccgcaccct gacactggac cgtgaggtga ccctgccgga gacaggtaca
       61 ccgacggtga acctgattaa cggcagcggt aagccggtga gcgtggccat cactgcacac
      121 cccgcgccgg accggataca ggtcagcacc ctgccggatg gcgtggagac atacggtgta
      181 tggggactct ccctgccgtc actgcgtcgt cgcctgttcc gctgtgtctc catccgggaa
      241 aacacggacg gcacctttgc catcacggcg gtgcagcacg taccggaaaa agaagccatc
      301 gtggataacg gggccagctt tgagccgcag tcaggcaccc tgaacagcgt tatccctccg
      361 gcagtgcagc acctgacggt ggaggtgagc gcggctgacg gtcagtatct ggcacaggcg
      421 aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagcgga
      481 agcggtgaga acagccgcct gctgaccacc gccatcactg ccgatacgga gcaccgtttc
      541 agtggcctgc ctctcgggga atacaccctg acggtcaggg cgattaacag ctatggccag
      601 cagggcgaac ccgccaccac cacgttccgg attaacgcac cggcagcgcc tgccggtgtt
      661 gaactgacgc cggggtattt tcagataacg gcagtcccga aactgaccat ttatgacccg
      721 acggtgcagt ttgagttctg gttttcggag gcaaaaattg cagacgccgc acaggtggaa
      781 acctctgccc gttatctggg gaccggcagt cagtggagtg tctccggtcc gcacattaag
      841 cccggaaagg atttctggtt ttatgtgcgc agcgtcaacc tggtggggaa atctgctttt
      901 gtggaagcca gtggccgggc cagcaatgat gcagaagggt atctggggct gtttcgggaa
      961 aaaataggaa aactgcatct ggctcagggg ctgtgggagc tgatagacaa cagccagctt
     1021 gcggatgaga tggcggagat gaagaccagc atcaccgaaa cccgcaatga aatcacacag
     1081 acggtcagta aaacactgga gaaccagagc gccaccatac agcagataca gcgcgtgcag
     1141 aaggacacaa atgatgacct ggctgcgctg tacatgctga aggttcaaaa aacgaaagac
     1201 ggcattccct atgtggccgg gattggtgca gggattgagg atactgatgg ccagccactg
     1261 agcaacatac tgctgctggc tgaccgtatc gcgatgataa atccggagag cggcaacagc
     1321 acgccgttat ttgtggcgca ggggaatcag ctgttcatga acgacgtgtt cctgaaacga
     1381 ctgtttgcgg tgagtatcac gtcatccggc aatcctccgg cattttccct gacgccggac
     1441 gggcgactga cggcgaaaaa tgcggatatc agtggcagtg tgaatgcgaa ctcagggacg
     1501 ctcaacaacg tcacgattaa tgagaactgt cagattaagg ggaaactgtc agccaaccag
     1561 attgaaggcg atattgtcaa aacggtcagc aagtctttcc cccgcacgag cacttatgcc
     1621 agtggcacca tcacggtaag aatcagtgat gatcagaagt ttgaccggca ggtcatgata
     1681 ccgccagtgt tattccgcgg tggtaagcat gagaatttca acagtaataa ccaacagtca
     1741 tactggtatt caacctgccg gttaagagtg acccgcaatg gtcaggagat ttttaatcag
     1801 tccacgacgg atgctcaggg cgtattttcc tcagttatag atatgcctgc cggacagggg
     1861 acgctgacac tgacattcac cgtatcttca tcaggagcga ataactggac accaacaacc
     1921 agtatcagcg atctgttggt tgttgtgatg aagaaagcca ccgcaggcat cagtatcagc
     1981 tgaattttat aacccatata cgggcgccag aaatggcgcc ttttttattg cagaaaagcg
     2041 agaggtaatt atgcgtaaac tttatgccgc cattttgtcc gcagccattt gtctggccgt
     2101 atccggtgcg cctgcatggg catctgaaca tcagtccacg ctgagcgcgg ggtatcttca
     2161 tgtctcgacg aacgttcctg gcagcgatga actgaacggg attaacgtga aataccgtta
     2221 tgagtttacg gacacactgg ggatggtgac gtcgttcagc tatgcaggag acaggaatcg
     2281 ccagctgacc cattacagcg atacccgctg gcatgaagat ttcgttcgta accgctggtt
     2341 cagcgtaatg gcggggccgt ctgtgcgcgt gaatgaatgg ttcagcgcgt atgcgatggc
     2401 gggcgtggct tacagccgtg tgtcgacttt
//
LOCUS       DAZFDP010000340         2404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFDP010000340 DAZFDP010000000
VERSION     DAZFDP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..282
                     /locus_tag="ACHJ1G_005361"
     CDS             <1..282
                     /locus_tag="ACHJ1G_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767008.1"
                     /translation="MPSNNIIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLI
                     ATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1153..1293)
                     /locus_tag="ACHJ1G_005362"
     CDS             complement(1153..1293)
                     /locus_tag="ACHJ1G_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4767009.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1595..1858)
                     /locus_tag="ACHJ1G_005363"
     CDS             complement(1595..1858)
                     /locus_tag="ACHJ1G_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4767010.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2281..>2404
                     /locus_tag="ACHJ1G_005364"
     CDS             2281..>2404
                     /locus_tag="ACHJ1G_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4767011.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          747 a          556 c          440 g          661 t
ORIGIN      
        1 atgccatcaa acaatataat tgaaataggc tacaatataa taaacaccga tctggtcatt
       61 ggcgaaaaaa ttcatttcag gacactaagc aatcgttctt tatattttaa cctcacccaa
      121 cccccattaa tcgccacaat aaaagatgat gtgttttgca ctcttcatga ttactataac
      181 cacaataatg aaacgaaatc cactattgac aattacatat caaaaataaa ggataatcat
      241 aataccccat ggttattaaa caacgaaaat gttcaggaat aaaaaaaaga gacaattccc
      301 gagataaaac aaaagagcat aaacatatga accggaaatt ctatttcaaa aatatcctgt
      361 ggggaatgtg ccccattata atatccatat tgcatctgat atatgacaca ccaacagaag
      421 catcgataaa agttgactta tttctgatac tcagtggcct ggcccttcct tgttcaatat
      481 acctgacaaa aacaatcaca ctaaaataca catccatatc attctggaaa aaacctcttt
      541 tctggtctta tggtatatat cattttcatc ttgtttatat aatgacatgt atagttttag
      601 caataccttc tgcaattatc gttttattct ttcactttta tcaaaacatc accagaaact
      661 ggaagtgaca accgcaataa tgaacataaa aaggcaaatc agaatggcag taaatcaata
      721 aagtaccatt tcatatgaat catcagaaaa acggtggacg cgacatccag cacgaggcaa
      781 aacacagcaa caaaataagc cggatatcgc gccctgtact tttctaactc aactcccggc
      841 attgtgcaag ttttaatcgc aggtggattc agagccacgc ccccattgat tcagcctgaa
      901 gacacgtata tccttggaga aagcaggaat ctcattgctg tgaaattcct tacagaatct
      961 tcagccccat tttatggggc tttttttggt gcccccacgc tgttaccaca ctttcgggat
     1021 gcgaataaaa aagcccacag tttttacact gcggactttt gttttttctt tgtgatttgt
     1081 gtgcaagggt gcgactttcg accgaacctt tggcaaccgg atagcgggga ctgttgtccc
     1141 cgctgcggtt tcttacggtt tacactgcaa gaacgccgca atttccgacc acatgaagct
     1201 taccctgaat tcacacggtg atgcgtgtaa cacccagacg gtcagcaaca tggtcaggca
     1261 taacatgccc acaatgatcg ttttttgcgg catagcactt gctcctttta cagagaggcg
     1321 ctaaccttcc acttgctgaa ggtagatggt cagggcctcg ggttaacgtt taagttgact
     1381 cggggccttt ccacatccgg ccttcaggta atccttccag ccgtcagccg aaaggcaccc
     1441 gcgcgtaatg tactggtttt cagagaggtc cgcaatcttc cggtgacgag atcatctgca
     1501 cacgccccgg tgggacgggc taacggtata ttcaggtgca ggatctctct gtctggtgcg
     1561 tttttaaatt gtcaaacgca ccctggcggc aaaatcagcc accttttttc gcatcaacaa
     1621 aacgcccaaa acgctgggta aagcgtgcaa cattaccttc tgaagcgaca gttctcagtt
     1681 tacctgtata aaacggatgc gacttagaag aaacgtcaat tgtaacatat gggtaagtta
     1741 caccatccag ttcaattacc cggtcagttt tgatcgtcga accgatcctg aagtactcat
     1801 caatactggt atcgtgaaat actactgtac ggtattccgg atggatattc ggtttcacaa
     1861 cagttttccc cctttaagtg aaaattccgc ataaaggtac atattcagac ggtcagattc
     1921 aactgtctgt tcttataacc tcactctctt tcagtattac aatatgttat ataattataa
     1981 actggacttc ccgaaaaaat ccattaaata agaagcgagc cgttgcggag gcttgctctc
     2041 tcctttaacc tcatgtgcaa tatccgtaac gcgctcacat aaatcatcat ctgataatat
     2101 aacaacaccc attattttat tctgacctaa aaacttctta tgaaataaac gtttacccga
     2161 cgtatctgat cccgctgttt ttacctgtat cctttaacat acttgagaag cccgctgaaa
     2221 aatataccgt aaaggcggtt ctctgtcttc cataaacctg tcggataaga agggcattaa
     2281 atgaaaacca aaaaagttat gatgattgca ctggtcagct ctgcgctggc tctttcagga
     2341 tgtggagcca tgggcacagc gatcaaaaaa cgaaatctgg aagtaaaaac ccagatgagt
     2401 gaaa
//
LOCUS       DAZFDP010000341         2394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFDP010000341 DAZFDP010000000
VERSION     DAZFDP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHJ1G_005365"
     CDS             <1..480
                     /locus_tag="ACHJ1G_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4767012.1"
                     /translation="LNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGNYVGA"
     gene            480..1133
                     /locus_tag="ACHJ1G_005366"
     CDS             480..1133
                     /locus_tag="ACHJ1G_005366"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4767013.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1130..1456
                     /locus_tag="ACHJ1G_005367"
     CDS             1130..1456
                     /locus_tag="ACHJ1G_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO4767014.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1453..1842
                     /locus_tag="ACHJ1G_005368"
     CDS             1453..1842
                     /locus_tag="ACHJ1G_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4767015.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTVGAAA"
     gene            1862..>2394
                     /locus_tag="ACHJ1G_005369"
     CDS             1862..>2394
                     /locus_tag="ACHJ1G_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4767016.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSR"
BASE COUNT          583 a          571 c          720 g          520 t
ORIGIN      
        1 ttgaatgaag ttcagaaatt cattgaagcc catccgggat gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt cagcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggaaccgga accacaacca gttcgagaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc acgggtgatt ttgtgcctga cccgccaggc tgaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcggtgctt acggaaaagc
      421 agcaagcgcg ctgcatcggg tgaagagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgagtaataa atattgccag gcgctggtgg aactgcggaa caaaccagcc catgaactga
      541 aggaagtggg cgatcagtgg cgcacgccgg acaacatttt ctggggaatt aacaccctgt
      601 ttggcccgtt tgttctggat ctgtttactg acggtgataa cgccaaatgt gccgcgtatt
      661 acacggcgga agataacgcg ctggcgcatg actggtcaga acgtcttgcg gagcttaaag
      721 gtgctgcctt tggtaatccc ccatacagcc gcgccagtca gcatgagggg caatacatca
      781 ccggcatgcg ttacatcatg aaacatgcca gtgccatgcg tgataagggc gggcgctatg
      841 ttttcctgat caaagctgcc accagcgaag tgtggtggcc ggaagatgcg gaccatattg
      901 cttttattcg cgggcgtatt ggttttgaac tgcctgcctg gtttatcccg aaggatgaga
      961 agcaggtgcc gacaggcgct ttcttcgctg gtgctattgc tgttttcgac aagacctgga
     1021 agggaccggc aatcagctac atcgggcgcg atgaacttga ggcatgtggt gaggcctttc
     1081 tggcgcaggt tcgccagcag gcggaaaaac tggtcaggga gatggcggca tgacgacgtt
     1141 aactcaatgc cagcagcagg tgctggatat gctgatttct taccagcaag aacgtggctt
     1201 tccgccaacc aatcaggagg tggcaaccat gctgggatac cgttcggtga atgcagcggt
     1261 ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg gcgtggcccg
     1321 ggggataact cttcataccg cggtgaagga cgacgacagc gaggcggtcg ggattatccg
     1381 cgcactgctt gccggtgagg aaaacgccag gctgcgtgca gcccactggt tacatgagag
     1441 gggcctgaaa gtatgaagct gatcttgcct ttccctccca gcgtgaacac gtactggcga
     1501 caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg gcgcaaattc
     1561 cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc gtctgccgaa accaacgtcg
     1621 gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg cgatctggac
     1681 aactataaca aggcgctgtt tgacgccctg acccatgcgg gtgtgtggga agacgacagt
     1741 caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa ggtcgagatc
     1801 actatcagta agtacgagaa aacggtgggt gcagccgcct gatcaagagg agaaacgaag
     1861 tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt atgggcgtta
     1921 cagcctgaac gatctgcaca gggcagccgg gggagaacaa aaaaaacgcc cgaaatactg
     1981 gctctccaat aagcaaacct gtgaattgat tgaacaactt ttcaccgagg gtggaattcc
     2041 gcctctggaa aagaatcaac cagttagcgt tattaatggc ggaaataacc aggggacgta
     2101 tgtctgcaaa gaactggtgt atgcctatgc aatgtggatc agcccgtcat tccatctgaa
     2161 ggtgatccgt actttcgata tggtaaccag cgcatcggaa aaattatccg ggcaggctgc
     2221 tgacaagatg caggctggcg tgattctgct ggactttatg cgccgggagt taaacctgtc
     2281 taactcttca gtgcttggtg cctgtcagaa actccaggag gctgttggct taccgaatct
     2341 ggcaccgcgc tatgccattg atgctcctgc tgacgcgcct gatggctcaa gccg
//
LOCUS       DAZFDP010000342         2384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFDP010000342 DAZFDP010000000
VERSION     DAZFDP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..569
                     /gene="espM1"
                     /locus_tag="ACHJ1G_005370"
     CDS             <1..569
                     /gene="espM1"
                     /locus_tag="ACHJ1G_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO4767017.1"
                     /translation="SFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFNVNKFS
                     GCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFSLV
                     SKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANVLADKF
                     YQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(788..1844)
                     /locus_tag="ACHJ1G_005371"
                     /pseudo
     CDS             complement(788..1844)
                     /locus_tag="ACHJ1G_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2076..>2384
                     /locus_tag="ACHJ1G_005372"
     CDS             2076..>2384
                     /locus_tag="ACHJ1G_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO4767018.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     E"
BASE COUNT          652 a          463 c          560 g          709 t
ORIGIN      
        1 tgtccttcag ctcttttggt atcagttatc ataaagataa ttctttccgg ggcaccatcc
       61 gtgggaagaa tgatgaggtc gtgaagtgtt caatgggaga gcgctctatc cgctttaatg
      121 ttaacaaatt tagcggctgt atactggaga cggtaagcag gcagagtact aaagatattc
      181 atggatgggt aagtgatgaa cggacagtat atccgtcaag ggttatcaac caagagattg
      241 ataattgctg ccttcaaaaa aatgcaaaaa tttcttctga agagaggaaa atggtctttt
      301 ctcttgtaag taaggagttt gaactaactc ttgatgttaa agcggcacaa agttctatta
      361 atcatataat aataggaaat gcttcttttg gcaaaaaaat ggatgctctt tgcgatggta
      421 tgagccgagc tgtaaaaaac agtacaacag attacatagc aaacgtgctt gcagacaagt
      481 tttatcagaa acatatcgcc ccgggtgttg atattgtaaa actacgaaat gaaatcccag
      541 gttatatgag tcgtgttata caggggtaag gttggaggag gctatgcgcc agtatcattg
      601 gttttcatcc tgtaaaaaga tatgagtaaa atatacaaca ggcgttacac tatatgtatg
      661 tatccaaata actcatagtt cacctgtaca gttataaatc gtaaagaaaa ttgcagcgcg
      721 tcgtacgcag aaacgtgctg tgattgacta tttatagctt tgtcacaacg acaagattca
      781 gtagtgttta cacgggcggg agaaatgacg gaataactaa atagctgcgc ctaatatcgc
      841 acatttttgc cagcccatgt ttgccttcgg ggaatggact gacggattcc ataaaatggc
      901 gaactttttc aatagttgcc acattgagcg gcactgatga ttacgcgtta ttgtggcgtg
      961 aagtatctgc catagctatt caaaatgatt cacccatgga gattaaaccg gctgataaat
     1021 gactctgcac ttcggtttat ccttcagcca gcgctgggtt tcccggcctt tgtggataat
     1081 gtagttgtcc tcgagcagcg tgatggtttt cgtccgacag tatgtagctt taagccgctt
     1141 cagcagtctg atgaacagcg ccgaactttt gctgttggcg cccacacagc tgacttaacc
     1201 tgtcccgctg tgcagcgcta cgtccagaga atatttttca ttttgttccg gcgtgacccc
     1261 tcgtttttgc ttcccgagca gtttccagtc cgcaccgatt ttgggattaa gatggataaa
     1321 tacttcatct tcataaaaga ccggatgctc tgtgctgcat tcgtccagtg cgttatggat
     1381 tgttgccatc tttttatctt taggcggggc acggatacgt agagttggcg cagcccttcg
     1441 ccacacaagc cccacagacg acaaccagcg gcgaatggtt ccagcatgta acaggcagcc
     1501 ggttatctca ttgattttta ttgtcagcag ttctgtactc cagcgtgaat aattgtagcc
     1561 aaagtcgcga gtgaatattt tacctgttca cgtaatagtg tgtagatatg cgcaaacggc
     1621 cagcggcggg ctcgctcggc aggtaatgat ttcagtccct caacacccga ctgtgtgaac
     1681 tagttaatcc agcgcccaac agaggaacgg gcgcagcata gtgtgcgggc aacgtcgctg
     1741 acacggtcgc cccggtgcag catcagcatg gctgtcagcc ttttatggat ggctttctgt
     1801 attaggcgtc gttcgtcacg ggaaattggt ggtatgatcg acattgctca gtccggttgg
     1861 tgatttgtat tgatttggcg attgatcaga tcgcacaatc gggttgagtt ccctctaaat
     1921 gatctactat tctgcgcagc tatttagtga gtatatattt tatgcttata aattagatta
     1981 aaataatcct acaattattc tgatggagta ttgctgtgga ggatggcttt ttgttaatta
     2041 tcgaaaagta agatatatgc tttagggata aaattatgcc attttcattt aatttgtctt
     2101 ctggaaatta cttgtcaaca caagatgttg aagttttaca acgtgcgacc agagatcatc
     2161 aaatggaaag actcacaata ggagaaagat cgttttctgt tcgctatcag agtgctatgg
     2221 acgcttttat tgtagatcct gttcaagggg aactatattc gggtttaagc catacagaac
     2281 tagccgatat cattagattg gctgattctg ttgaaaatca attgaatgga ggcaattcat
     2341 ttcttgatgt attcagtaca tatatggggc aggttatttc tgaa
//
LOCUS       DAZFDP010000343         2358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFDP010000343 DAZFDP010000000
VERSION     DAZFDP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            227..1087
                     /locus_tag="ACHJ1G_005373"
     CDS             227..1087
                     /locus_tag="ACHJ1G_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO4767019.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1235..1450)
                     /locus_tag="ACHJ1G_005374"
                     /pseudo
     CDS             complement(1235..1450)
                     /locus_tag="ACHJ1G_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1562..1942)
                     /locus_tag="ACHJ1G_005375"
     CDS             complement(1562..1942)
                     /locus_tag="ACHJ1G_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFO4767020.1"
                     /translation="MALKPRELSKGNAGAAGLYAFFAEHIDPTCGAVVCDQQFLADHL
                     EVSTRTIRNWLKYLETERALVRIPVAGRVCAYALDPHEVWKGYDNAKEYAAFVTKTLV
                     NKDGDIKRRIMSMFSPEERPDDRE"
BASE COUNT          610 a          549 c          560 g          639 t
ORIGIN      
        1 gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      241 ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      421 aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg ttgacgccgg
      481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      601 aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag gaccgaagga
      661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
      781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1201 acgtgagttt tcgttccact gagcgtcaga cccctagaaa gccatgctcc agccgcccgc
     1261 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     1321 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     1381 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     1441 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     1501 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     1561 gtcactccct gtcgtctggg cgttcctctg ggctaaacat cgacatgatc cggcgcttaa
     1621 tatcgccgtc cttgttgacc agcgtcttgg tcacgaaggc cgcgtattct ttggcgttgt
     1681 cgtacccttt ccagacctcg tgggggtcaa gagcgtaggc gcaaacccgc ccagcgactg
     1741 ggattctgac caaggcgcgt tctgtctcaa ggtacttgag ccagttccga atcgtgcggg
     1801 tgctaacctc cagatggtca gccaggaact gctgatcgca gacaacagcg ccacaggtag
     1861 ggtcgatgtg ttcagcgaag aaggcataca ggccagcggc ccccgcattg ccctttgaca
     1921 gctcccgggg tttgagggcc aaccgtacga aaatgtacgg taagaggaaa attatcgtct
     1981 aaaaatcgat tagtagacaa tgccgtccgt taagtgccaa ttttcgatta aaaagacacc
     2041 gttttgatgg cgtttttcta tgtccattat gtcttgatat atcatacagt tacttcacta
     2101 acgtacgttt tcgttccatt ggacttcaga ccccgtgtta cgaacctgaa tagcaaaaaa
     2161 gaatctgttt attattgtaa gtccattggc attgatcgcc ttagttattt atcggtaaaa
     2221 atggcttgtg ataaatattc agctttaggg tgtgataaat gaaacatcta aaataggggt
     2281 cagtttggat atcgaaaaat taggatacat tctttcagaa aggtggcacc tatgaacagg
     2341 tgccaataat taaatctg
//
LOCUS       DAZFDP010000344         2348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFDP010000344 DAZFDP010000000
VERSION     DAZFDP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            255..344
                     /locus_tag="ACHJ1G_005376"
                     /pseudo
     CDS             255..344
                     /locus_tag="ACHJ1G_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            818..919
                     /gene="ykgR"
                     /locus_tag="ACHJ1G_005377"
     CDS             818..919
                     /gene="ykgR"
                     /locus_tag="ACHJ1G_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO4767021.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1283..1546
                     /locus_tag="ACHJ1G_005378"
     CDS             1283..1546
                     /locus_tag="ACHJ1G_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4767022.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            1546..1686
                     /gene="ykgO"
                     /locus_tag="ACHJ1G_005379"
     CDS             1546..1686
                     /gene="ykgO"
                     /locus_tag="ACHJ1G_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO4767023.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1721..1948)
                     /gene="ykgL"
                     /locus_tag="ACHJ1G_005380"
     CDS             complement(1721..1948)
                     /gene="ykgL"
                     /locus_tag="ACHJ1G_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO4767024.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
BASE COUNT          706 a          513 c          441 g          688 t
ORIGIN      
        1 gtgaataaca atgacctgcc tttaattaaa actattgaaa atacgtcgaa ataggtatat
       61 atgccaggat aaaccaatat attttttatg gatattcatt ttccttcatc ggactgtaaa
      121 ttgcggagat atcgaacttg cactacccat cgcaacactc aaacagattt atgaatttcg
      181 ttcataaccc ctgacaaatt ctgccgtcta atcgagcttt tccctttacg ttatgcttct
      241 catgaacgat aacacaactt gttcatgaat taaccattcc ggataaacta tgggatcaaa
      301 ttcaggcatc gaaaggaact ggctggtttc cgttaatgga ttaaatatct attattctcc
      361 cgagagtaaa actatcaaat caatgagtaa ccgatacagc ctcacaaatt ctttcagata
      421 attaacccgt cactgcggca cgttctctca taaggcgtgc ctgcagtgta atccagtatg
      481 tatccggcat agttgacggt ttaatagaac tcaattcctt tcccaggtta ccaacaacga
      541 tatcttgtat aacaccccag gagatacgac attatgatga aatgaggtat gctcctcagc
      601 gccaatcaac gcccggactg ggccagtctt gagatcagac tcattcccgg aactgacttc
      661 agtcgacata tatcaatgac gttggtgcaa taaaagatag ctgatgtaat acatctattc
      721 aaatttgtga tacatgtcaa aaaattggtt gaccaaactc gttatttata taagggcact
      781 tacgaagtgc actctttttt aaagcgagga agtaccaatg aaagagaata aagtacaaca
      841 aatcagtcat aaactgatta atatcgttgt ttttgtcgca attgtagaat acgcctattt
      901 atttctccat ttctattaat aacggcaata aactgttcac ttcagtgata tttaaaatat
      961 gcatcctctc cctttttgta agtaattatt atatccgtgg gagaggaata cacgctgtca
     1021 ggtaatcaat catactgcga taaatcatcg gccagtaaag tggagataac ctccattctc
     1081 gaaaaatcca tactctcagc aaaaccatca tcaatcactc atccaggcgt ttatgggagc
     1141 gtcgccaacg gctgctaaca atgccagact tccccgttgg ggaaattcca cataccacaa
     1201 atgggcacag ggattgggtg ttgaaatgat ccggatgagc atgtatcttt acggttatgt
     1261 tataacataa caggtaaaaa tgatgaagcc caatatccat cctgagtatc gtactgtggt
     1321 gttccacgac accagtgttg atgagtactt taaaatcggc tcgactatca aaacagaccg
     1381 tgagattgag ctggatggcg taacgtatcc gtacgtgaca attgatgtct cttctaaatc
     1441 gcacccgttc tatacaggga agctgagaac agtgacatca gaaggaaatg ttgcacgatt
     1501 cacccaacgt tttggtcgtt ttgttagcac gaaaaagggg gcgtgatgaa agttcttaac
     1561 tctctgcgta ccgcaaaaga acgccatcca gactgtcaga ttgtgaagcg aaaaggacgg
     1621 ttgtatgtga tttgtaaatc taatccacgt tttaaggccg ttcagggtcg taagaaaaaa
     1681 cgttgattca aaattcgacg aattaacgat atttgtctga ttaataatca gatcggatta
     1741 atgttggtgt gtttataaca ccaacattaa ttttcctggg gatatattct tcctgttcat
     1801 ttgaggccaa ctgcctgacg tttctctccg aatattccat tatcttaatg ttgacttgtt
     1861 gaccagcttc gcccctgtat gctggcatca accctctttt agactgaaca cgccactcag
     1921 tctcctccct ttgcggcgca gcctgcattt tcactcaaac tgttaagatg ataaatgtgg
     1981 taaatctgtt ggtactaaca taaaaacgtt tacgccacag gaacagtctg atccaccggt
     2041 aacccgtagc cgacgttcga gtgccagtta gagtaacgcg cacagataac tgcactcagt
     2101 gccctgacaa agaggccatc gttcctgtga cagctggcag ccttcgttta acttcactta
     2161 atctggctct tgggggctta ccgaacagat gacgtacata cgcccgttca attttccatc
     2221 acttattgta atgaacacct ataaccattt tgtgcggcat gttaatccat taaaatttct
     2281 tactgattgg caaatcatct tcaatgacag ctcattatag ttttatattc tatcccttac
     2341 ccttaaaa
//
LOCUS       DAZFDP010000345         2335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFDP010000345 DAZFDP010000000
VERSION     DAZFDP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..518
                     /gene="pal"
                     /locus_tag="ACHJ1G_005381"
     CDS             3..518
                     /gene="pal"
                     /locus_tag="ACHJ1G_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4767025.1"
                     /translation="MNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMDAN
                     GGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYKVT
                     VEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAA
                     YSKNRRAVLVY"
     gene            528..1319
                     /gene="cpoB"
                     /locus_tag="ACHJ1G_005382"
     CDS             528..1319
                     /gene="cpoB"
                     /locus_tag="ACHJ1G_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4767026.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            1484..1559
                     /locus_tag="ACHJ1G_005383"
     tRNA            1484..1559
                     /locus_tag="ACHJ1G_005383"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1517..1519,aa:Lys,seq:ttt)
     gene            1695..1770
                     /locus_tag="ACHJ1G_005384"
     tRNA            1695..1770
                     /locus_tag="ACHJ1G_005384"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1728..1730,aa:Val,seq:tac)
     gene            1773..1848
                     /locus_tag="ACHJ1G_005385"
     tRNA            1773..1848
                     /locus_tag="ACHJ1G_005385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1806..1808,aa:Lys,seq:ttt)
     gene            1998..2073
                     /locus_tag="ACHJ1G_005386"
     tRNA            1998..2073
                     /locus_tag="ACHJ1G_005386"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2031..2033,aa:Val,seq:tac)
     gene            2077..2152
                     /locus_tag="ACHJ1G_005387"
     tRNA            2077..2152
                     /locus_tag="ACHJ1G_005387"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2110..2112,aa:Lys,seq:ttt)
BASE COUNT          633 a          569 c          608 g          525 t
ORIGIN      
        1 aactgaacaa agtgctgaaa gggctgatga ttgctctgcc tgttatggca attgcggcat
       61 gttcttccaa caagaacgcc agcaatgacg gcagcgaagg catgctgggt gccggcactg
      121 gtatggatgc gaacggcggc aacggcaaca tgtcttccga agagcaggct cgtctgcaaa
      181 tgcaacagct gcagcagaac aacatcgttt acttcgatct ggacaagtac gatatccgtt
      241 ctgacttcgc tcaaatgctg gatgcacatg caaacttcct gcgtagcaac ccgtcttaca
      301 aagtcaccgt agaaggtcac gcggacgaac gtggtactcc ggaatacaac atctccctgg
      361 gtgaacgtcg tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt tctgcagacc
      421 agatctccat cgtttcttac ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg
      481 catactccaa aaaccgtcgt gcggtactgg tttactaaga gaattgcatg agcagtaact
      541 tcagacatca actattgagt ctgtcgttac tggttggtat agcggccccc tgggccgctt
      601 ttgctcaggc accaatcagt agtgtcggct caggctcggt cgaagaccgc gtcactcaac
      661 ttgagcgtat ttctaatgct cacagccagc ttttaaccca actccagcaa caactttctg
      721 ataatcaatc cgatattgat tccctgcgtg gtcagattca ggaaaatcag tatcaactga
      781 atcaggtcgt ggagcggcaa aagcagatcc tgttgcagat cgacagcctc agcagcggtg
      841 gtgcagcggc gcaatcaacc agcagcgatc aaagcggtgc ggcggcatca acgacgccga
      901 cagctgatgc tggtactgcg aatgctggcg cgccggtgaa aagcggcgat gcaaacacgg
      961 attacaatgc agctattgcg ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg
     1021 catttcagaa tttcatcaaa aattaccctg attcaactta cctgccaaac gccaattatt
     1081 ggctgggtca gttaaactac aacaagggta aaaaagatga tgcggcgtac tattttgctt
     1141 cggtagtgaa aaactatccg aagtcaccaa aggctgctga tgcgatgttt aaagtcggcg
     1201 tcatcatgca ggacaaaggt gacaccgcaa aagcgaaagc cgtataccag caggttatca
     1261 gtaaataccc tggtactgat ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat
     1321 gcataacaca cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt
     1381 tgagtgatct gcatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc
     1441 cgttgccacg ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt
     1501 ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa
     1561 tcgctaaggt ggaagcggta gtaaaacgtg aaggataacg ttgcatcagc aacggcccga
     1621 agggcgagac gaagtcgagt catcctgcac gacccaccac taacatagtt agttgtagta
     1681 tccagcgtag tattgggtga ttagctcagc tgggagagca tctcccttac aaggaggggg
     1741 tcggcggttc gatcccgtca tcacccacca ccgggtcgtt agctcagttg gtagagcagt
     1801 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccagt tttagcatcg
     1861 aagacagatg ttaagcgtgt aggataacgt tgcgtcagca acggcccgta gggcgagcga
     1921 agcgagtcat cctgcacgac ccaccactaa ttacggtggg ttcggtggaa gtagtttgta
     1981 gtatccagcg cagtatcggg tgattagctc agctgggaga gcacctccct tacaaggagg
     2041 gggtcggcgg ttcgatcccg tcatcaccca ccactcgggt cgttagctca gttggtagag
     2101 cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caattttaac
     2161 atcaaactca gatgttaagc gtgaaggata acgttgcgcc agcaacggcc cgtagggcaa
     2221 gcgaagcgag tcatcctgca cgacacacca atcttaaaga ttggccccga gtagaaatct
     2281 ttcaggtaac acccgtatgg gtcgttagct cagttggtag agcagttgac tttta
//
LOCUS       DAZFDP010000346         2314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFDP010000346 DAZFDP010000000
VERSION     DAZFDP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..>2314)
                     /locus_tag="ACHJ1G_005388"
     CDS             complement(20..>2314)
                     /locus_tag="ACHJ1G_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4767027.1"
                     /translation="MEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          418 a          740 c          593 g          563 t
ORIGIN      
        1 tttttcatat ttcctcctgt taattgaccc agcattcttg ccacgttcta caagccagag
       61 tcataactga agcgtcaaca cctgcaccga taccaacaga ttctgtcgat gattctggat
      121 tatcaacatc cactacataa gtatacgcaa aatgcccccc aactccgccg ccaaccgtta
      181 cacctactga attgctgact cccgatgcag gtaaagactt ggtctcagaa tatgtgatag
      241 ccgcatttat cccaccacca attcctgcat gtccgcatgc tgtagcataa atacaaatat
      301 cagataacat ttcacctttc tttttagcaa acgcaacgcc aagtcctgca tgaccaccta
      361 atgatattag atctccactg ccagtaatat caattgatga tgttccatat gaatcatcat
      421 gccaaacatc atataataat tgatataaat tatacaaacc caaaggatcc atccttatta
      481 tcggattcag tgggtaatta tacaggttcc atccccccgc cagcccaatg gggtcttgtg
      541 taatatacct cccctgtagc gggtcatagt accggttccg gttatagtac agccctgatt
      601 cctcatcatg ctgctgcccc ggcagacggt atggctggtg caggtaatac gggttctctt
      661 cattaagctg gttgccccat tcatcatact ccccgcgcca cgccgtattg ccgtcttcgc
      721 tgatgagcgc cagcggcagg ccccggtggt cgcagtggta aaaatgaact tttcgcgccg
      781 gtgtgtattc cggctccacc tgtctggcca gttgctccac cgtcagcccg cactgcgcaa
      841 gccacgcccg gctttcactg ctcacgcggt ctgcccggat ttcttcctcc agcctgtcca
      901 gcagccgcac cagttcagcc ggaaatacca cgccgtgccc gtcctcgctc ccttcctgct
      961 gcagcttctc cgccaggctg cggcgctgcg ctttctcccg ctcgccgttg tctgtttcga
     1021 tgcggatgag cggcgcgaag ctccccggct gatataccgt ctggatacgc gtggtgtcgg
     1081 tctgtaccgt cgtcagccgg tcgccgtccc acccgtacca cgtcatctcc ggtttacgcg
     1141 acagcgacat ccaccccgtc aggtcacgct cccgccgcca tacccgtttc gccattcgcc
     1201 gtcccagcgg gtcgtagagg tagcggctct cgaccagtgg ctcgccatgc tgtatccgcg
     1261 tgtagaacac cagacggtgc tggctgtcgt agtggtagtg gtgggtccgc tcgtcgtccg
     1321 tccggataac acccgccggg ataaggtccg tcttctccgt cagtctgccg tattcatcat
     1381 agtggtaaac atagtgcgca tcctctgcga tgcggttatc cggccacgcc gtgagggtac
     1441 tgtccgggtg cagctccggg tccggcaggc ggtttcccgc cgggtcggtg gcatacggga
     1501 tgcggatgtc caggtccggt gcgagggtac gcacgctctc cagcctgccc gtggcgctgt
     1561 agccgtattc ccgcgtctgt cgcgggccgc tgatgcgcac caggtcgccg ttgtcattcc
     1621 acccgtagtc acggtcatac accaggctgt tcaggtgctg gctctgtaac tggcctgcgg
     1681 gggtgtatgt gctggtcagt ttatatgcgg cattactgcc tgccatgctg ccaaagctgc
     1741 gcaccgtctc ccggtgcatg cggtcgcgcg tgtactccag cagcggcgtg tcgccaagct
     1801 tcatgcccgc cagataaccg ctgccgtagg tcagccactc caccggcggc aggctgtccg
     1861 gcgtgacgcg gtttgccagc ccctgttcgt tgtaggcgtg tgttgtctca tgctgccaca
     1921 gcagttcccc cgtctccggg ttctccaccg tctgccgttc gccggtcagg cggcccttat
     1981 cgtcgtagcc gtagtggaca gcgacacggt ggccttcgct caggtggctg atgtctgtca
     2041 gccagccgtg gtcatcatac tgccactgct ctgccggttc gccgttcacc gtgcggtgag
     2101 tgatacggtc cgattcatcg tagtaccaga gggtgacaag cccctcatct tcactctgcg
     2161 tgagttttcc ggtcaggtca taatgataac gttgcgtccg cccgtcaaag ccgccctgct
     2221 gtaccaaccg gtccagcgca tcgtagctga agtcgctgtg gctgccgttc tcgttagtca
     2281 gactgatgac acgtccggca gcatcgtact ccat
//
LOCUS       DAZFDP010000347         2303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFDP010000347 DAZFDP010000000
VERSION     DAZFDP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1800
                     /locus_tag="ACHJ1G_005389"
     CDS             <1..1800
                     /locus_tag="ACHJ1G_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4767028.1"
                     /translation="YLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTAYTPAGQLQ
                     RQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTSVHTTAANLDIRIPYA
                     TDPAGNRLPDPELHPDSALSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTD
                     DERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSL
                     SRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLAD
                     TLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMD
                     PVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLP
                     GQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASK
                     YGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYD
                     YTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFP
                     DINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1797..>2303
                     /gene="ybfC"
                     /locus_tag="ACHJ1G_005390"
     CDS             1797..>2303
                     /gene="ybfC"
                     /locus_tag="ACHJ1G_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4767029.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNK"
BASE COUNT          636 a          598 c          608 g          461 t
ORIGIN      
        1 tacctggcag gcatgaaact cggcgacaca ccgctggtgg agtacacccg cgaccgcctg
       61 caccgggaaa cgctgcgccg cttcggccgt tatgaactca ccaccgctta tacccctgcc
      121 gggcagttac agcgtcagca cctgaacagc ctgcagtatg accgcgatta cacctggaac
      181 gacaacggcg aactcatccg catcagcagc ccgcgccaga cccggagtta cagctacagc
      241 accaccggca ggctgaccag cgttcacacc accgcagcga atctggatat ccgcatcccg
      301 tatgccacag acccggcagg taaccgcctg cccgacccgg agctgcaccc ggacagcgcc
      361 ctcagcatgt ggccggataa ccgtatcgcc cgtgacgcgc actatcttta ccggtatgac
      421 cgtcacggca ggctgacgga gaaaaccgac ctcatcccgg aaggggttat ccgcacggat
      481 gatgagcgca cccaccggta ccattacgac agtcagcacc ggctggtgca ctacacgcgg
      541 acacaatatg cagagccgct ggtcgaaagc cgctatcttt acgacccgct gggccgcagg
      601 gtggcaaaac gggtgtggcg acgtgaacgg gacctgacgg gctggatgtc gctgtcacgg
      661 aaaccgcaag tgacctggta cggctgggac ggcgaccgcc tgaccacaat acagaacgac
      721 agaacccgca tccagacgat ttatcagccg gggagcttca cgccactcat cagggttgaa
      781 accgccaccg gtgagcaggc gaaaacgcag cgccgcagcc tggcggatac ccttcagcag
      841 tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca gatgctcgac
      901 cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg ctggctggca
      961 tcgtgcggcc tgacggtgga gcagatgcaa aaccagatgg acccggtgta cacgccggcg
     1021 cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggcgct tgtcagcacg
     1081 gaaggggcaa cagaatggtg cgcagaatac gatgaatggg gcaacctgct gaatgaagag
     1141 aacccgcatc agctgcagca gcttatccgc ctgccggggc agcagtatga tgaggagtcc
     1201 ggcctgtatt acaaccgcca ccgctattat gacccgctgc aggggaggta tatcactcag
     1261 gatccgattg ggctgaaggg gggatggaat ttttatcagt atccgctgaa tccggttcag
     1321 tatatagatt caatgggact ggcatcaaaa tatggacact taaataatgg cggatatgga
     1381 gcgagaccca acaaaccgcc tacgcccgat ccaagtaaat tgccggacat agcgaaacaa
     1441 ttaagactgc catatcctat tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc
     1501 ttcagagcat taagccctta cgactacaca ctgtattgca ggaagtgggt aaaaccaaat
     1561 ctgacttgta cgccacagga tgattcccag tatccaggga tggatacaaa gacagcaagt
     1621 gattacctgc cacagacaaa ttggccaaca actcaattac caccaggata tacttgtgca
     1681 gaaccctatt tattcccaga cattaataaa cccgatgggc cagcaacagc agggatagat
     1741 gatttgggtg aaattttagc taagatgaaa cagagaacat cgagaggaat aagaaaatga
     1801 aaagagtttt gttctttttg ctgatgatat ttgttagttt tggtgttata gctgattgcg
     1861 aaatacaagc taaagatcat gattgtttta ctattttcgc taagggtact attttttccg
     1921 cgtttcctgt cttaaataat aaagccatgt ggagatggta tcagaatgag gacatcggtg
     1981 agtattattg gcaaacagaa ctgggtacat gtaaaaataa taaattcaca ccaagcgggg
     2041 cgagactctt aattcgcgtc gggtcactac gccttaatga aaatcatgcc atcaaaggaa
     2101 cgttgcagga gttgataaat acagcagaaa aaacggcgtt tctcggcgat cggtttagaa
     2161 gttatataag agcgggtatc tatcaaaaaa agagcagtga tccagttcag ttgttggcag
     2221 tccttgataa ttcaatcatg gtaaaatatt ttaaagatga gaaaccaact tatgctcgaa
     2281 tgacagcaca tctcccgaac aaa
//
LOCUS       DAZFDP010000348         2274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFDP010000348 DAZFDP010000000
VERSION     DAZFDP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..131)
                     /locus_tag="ACHJ1G_005391"
     CDS             complement(<1..131)
                     /locus_tag="ACHJ1G_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4767030.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYNSQWAAICSIAPKIGCTP
                     "
     gene            937..1914
                     /locus_tag="ACHJ1G_005392"
     CDS             937..1914
                     /locus_tag="ACHJ1G_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4767031.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            2098..>2274
                     /locus_tag="ACHJ1G_005393"
     CDS             2098..>2274
                     /locus_tag="ACHJ1G_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4767032.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          605 a          558 c          542 g          569 t
ORIGIN      
        1 ggtgtacagc caatctttgg ggcaatggaa caaattgccg cccattgtga gttatattcg
       61 ccctgacttt ccagaaccat acgaactgcc cgttgacgga cctcggggga aaaacgtgta
      121 tttttagtca tcctgtttac ctctttctca gggagtttgg tctccaggat tcccggggcg
      181 gttcagtgga taaatgctta ttacaacgtt tatcgacctc atacaaacaa tggtggttta
      241 tcgccttgcc gcatgaagaa aaatggaaac aggttattcc ggtgtcctga ttttatgatc
      301 cactacacat tcctgacgaa cgccactgca taaatcacat tgcacatcct cagagagtac
      361 gcagtatagg aggggtatat atacattatc ggtatatcag gcgacttctg tctgtatgtt
      421 gtcggtgctc gccgcagacc ggtgaccggg cgaagcccgc gaaccggcga ggaagcgcaa
      481 tgtatctgta gttacagtga gcagatacgc cctgaagctt cctcataaaa cagttgtgta
      541 aactgttttc ccggaatgat ttatccacat atccggcggc ccgatccagt aaacagatcc
      601 ataaatgatc aagaaatgat ccattaaaga tccccatatc gctgcaaacc ttgtcactca
      661 tgctccggga ccacgatcac ataagctgtg gcatgtttct gataaactgt agcaggatca
      721 tgataagctg tattcagtaa tgtatatact gtagtaagtt aatgacataa attatggtca
      781 gtacgccagg ctcagctgtt aaatacagac tgcaggtttt tcttcagtca gttgacgggg
      841 ttctgacact caattttctg tttattcttt tactgtccac aggcaggagg ctttctggaa
      901 aacgaaaatt cagacatcaa aaaactgttc ggcgaggtgg ataagtcgtc cggtgagctg
      961 gtgacactga caccaaacaa taacaacacc gtacaacctg tggcgctgat gcgtctgggc
     1021 gtttttgtac cgacccttaa atcactgaag aacagtaaaa aaaatacact gtcacgtact
     1081 gatgccacgg aagagctgac acgtctttcc ctggcccgtg ctgagggatt tgataaggtt
     1141 gagatcaccg gcccccgcct ggatatggat aacgatttca agacctgggt gggaatcatt
     1201 cattcctttg cccgccataa cgtaattggt gacaaagttg aactgccttt tgtcgagttt
     1261 gcaaaactgt gtggtatacc ttcaagccag tcatcccgca ggctgcgtga gcgcatcagc
     1321 ccttccctga aacgcattgc cggtaccgtg atctcctttt cccgcaccga tgagaagcac
     1381 acccgggaat atatcaccca tctggtacag tcagcctact acgatactga acgggatatt
     1441 gttcagttac aggctgatcc ccgccttttt gaactgtacc agtttgaccg gaaagtgctg
     1501 ctgcagctca aggccatcaa tgccctgaag cgacgggagt ccgcccaggc actttacacc
     1561 tttatagaga gcctgccccg ggatccggca ccgatatcgc tggcgcggct gcgtgcccgc
     1621 ctcaatctga agtctcctgt attttcccag aaccagacgg tcagacgggc aatggagcag
     1681 ttgcgcgaga ttggatatct tgattacacg gagatccagc gggggcggac aaaattcttc
     1741 tgtattcact accggcgtcc ccggttaaaa gcgccgaatg atgagagtaa ggaaaatccg
     1801 ttgccacctt cacctgcgga aaaagtcagt ccggagatgg cggagaaact tgccctgctt
     1861 gaaaaactgg gcatcacgct ggatgacctg gaaaaactct tcaaatcccg ctgaacataa
     1921 actgtagtca gtgaagagtg ctcctttact gactacagct tatattatca ggtgcagtga
     1981 gtggtctgct cactgcagtt tatatgtaag cgtacagcct gaaccgtctg gtcagaatct
     2041 gacgaattag acaaagtggt gtccaccaaa taagtagtgg gaaccaaagt gtcagatatg
     2101 cagaaaaatg tgactcccgg caggcgaaaa ggctgcccta attatcctcc cgaatttaaa
     2161 cagcagctcg ttgctgcctc ctgtgaaccc gggatatcca tctcaaaact tgctcttgaa
     2221 aatggcatta acgccaatct gttgttcaaa tggcgacaac aatggcgcga ggga
//
LOCUS       DAZFDP010000349         2266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFDP010000349 DAZFDP010000000
VERSION     DAZFDP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..909
                     /locus_tag="ACHJ1G_005394"
     CDS             <1..909
                     /locus_tag="ACHJ1G_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO4767033.1"
                     /translation="RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHP
                     DKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGL
                     AGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIID
                     VFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEA
                     GLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL
                     GRLGHTPPAEAEKAYYASIGNDDLAA"
     misc_feature    <1..93
                     /locus_tag="ACHJ1G_005394"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1250..1543
                     /gene="iss"
                     /locus_tag="ACHJ1G_005395"
     CDS             1250..1543
                     /gene="iss"
                     /locus_tag="ACHJ1G_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO4767034.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1634..1816)
                     /gene="rzoD"
                     /locus_tag="ACHJ1G_005396"
     CDS             complement(1634..1816)
                     /gene="rzoD"
                     /locus_tag="ACHJ1G_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4767035.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2033..>2266)
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005397"
     CDS             complement(2033..>2266)
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005397"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4767036.1"
                     /translation="YIEVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGAC
                     DQLRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          572 a          544 c          591 g          559 t
ORIGIN      
        1 cgaccgcctc tggaaaaaat gatgccactg ctggataagc tgcgtgagca gtacggggtc
       61 ggaccggtat gcagcgaact gcatattgcc ccgtcaacgt attaccattg tcagcaacag
      121 cgacatcatc cggataaacg cagtgcccgt gcgcagcacg acgactggct gaagagagag
      181 atacagcgcg tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag
      241 ttgttacggg aaggaatcag ggtggccaga tgtacagtgg cacgtctcat ggcggttatg
      301 ggacttgccg gtgttctccg gggtaaaaag gtccgtacga ccatcagccg gaaagccgtt
      361 gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac gacctgacca gctgtgggtg
      421 gctgatttta cttacgtcag cacatggcag ggcttcgtct atgtggcgtt tatcattgat
      481 gtgtttgccg gatacatcgt ggggtggcgg gtctcatcgt ctatggaaac gacattcgtg
      541 ctggatgcgc tggagcaggc gttgtgggcc cgtcgtccgt ctggcaccat ccatcacagc
      601 gataaaggct ctcagtatgt gtcactggcc tatacggagc gactaaaaga agccggatta
      661 ctggcatcaa cagggagtac aggcgactcg tatgacaacg cgatggctga gagcatcaat
      721 ggtctttaca aagcggaggt aatacaccgt aagagctgga aaaaccgtgc agaagtggaa
      781 ctggccacac taacgtgggt ggactggtat aacaatcgac gattgctggg aaggctgggc
      841 catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa cgatgatctg
      901 gcagcctgag ttcacagata aaacactctc caggaacccg gggcggttca gtcagcacag
      961 taaaacccgg tgtgcatcgt ttttgattat tcccgcacac tcacgcagaa ggaattcccc
     1021 gtcgggctac ggtcatggtt aatgcgggaa tacggcgacg atacagcgca gctaaaaggg
     1081 taatggacag atagagcggt ttatttcatt ccacaggatt ctgagtgccc ccctcctcca
     1141 ataggctgag catccaccta tatagtttta attttcatca atccatttaa ctatcgttta
     1201 attgttgtca cataggattc tgccgttttt aacaatgcag gataataaga tgaaaaaaat
     1261 gttgttttct gccgctctgg caatgcttat tacaggatgt gctcaacaga cgtttactgt
     1321 tggaaacaaa ccgacagcag taacaccaaa ggaaaccatc acccatcact tcttcgtttc
     1381 gggaattgga caggagaaaa ctgttgatgc agccaaaatt tgtggcggcg cagaaaatgt
     1441 tgttaaaaca gaaacccagc aaacattcgt aaatggattg ctcggtttta ttactttagg
     1501 catttatact ccgctggaag cgcgtgtgta ttgctcacaa taattgcatg agttgcccat
     1561 cgatatgggc agctctatct gcactgctca ttaatatacg tctgggttcc ttccagttgt
     1621 ttttgcatag tgatcagcct ctctctgagg gtgaaataat cccgttcagc ggtgtctgcc
     1681 agtcgggggg aggctgcatt atccacgccg gaggcggtgg tggcttcacg cactgactga
     1741 cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa catcatcacg cagagcatca
     1801 ttttcagctt tcgcatcagc taactccttc gtgtattttg catcgagcgc agcaacatca
     1861 cgctgacgca tctgcatgtc agtaattgcc gcgttcgcca gcttcagttc tctggcattt
     1921 ttgtcgcgct gggctttgta ggtaatggcg ttatcacggt aatgattaac agcccatgac
     1981 aggcagacga tgatgcagat aaccagagcg gagataatcg cggttactct gttcattgct
     2041 gaccccacaa acagatttca cgctcaatct cacgacgagt catgagacct ttccattgct
     2101 taccgccagc atatgtccag cgacgtagct gatcacatgc gcctttgata tcgccctggt
     2161 ttattttgcg aagaagcgtc gatgttctga aattgccagc acccacgttg taaacgaatg
     2221 agtaaagagc gccgcgcgtt gtttccggta tatcgacttc gatgta
//
LOCUS       DAZFDP010000350         2240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFDP010000350 DAZFDP010000000
VERSION     DAZFDP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..393)
                     /locus_tag="ACHJ1G_005398"
     CDS             complement(19..393)
                     /locus_tag="ACHJ1G_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767037.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_005399"
     CDS             complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHJ1G_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4767038.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHJ1G_005400"
     CDS             complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHJ1G_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO4767039.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(794..949)
                     /locus_tag="ACHJ1G_005401"
     CDS             complement(794..949)
                     /locus_tag="ACHJ1G_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4767040.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1222..1938)
                     /locus_tag="ACHJ1G_005402"
     CDS             complement(1222..1938)
                     /locus_tag="ACHJ1G_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4767041.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1988..2203
                     /locus_tag="ACHJ1G_005403"
     CDS             1988..2203
                     /locus_tag="ACHJ1G_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4767042.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          565 a          484 c          490 g          701 t
ORIGIN      
        1 acctcacctg tgaataaatt aatgattaat tgatattttg ttgtttgatt caactttccc
       61 atcggatgtg tgatgcttta aatcacagga attaatactg cttgctgtaa aatgattttc
      121 aaggggagct attcgaatcc ctttcttttt cattaacaag ccaaatcctt tattaatgat
      181 gtccattaat tccagaaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc
      241 ttcgtacagc ccgataaagg ctcggcgtac gttaccagat atattgtcga tggtttcttt
      301 ttctacggta ctcaggtcaa gagtcgccag ttgagagcga accacattcg ctgccatttc
      361 ctggaatggc attggtaaat ctttaaattc cattattagc ctcgttggtt agctattaac
      421 gcgggtatgt aatcattctg gcaatgctta atgccgctgc tttttccagc ctggtgatat
      481 cctgctccag agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca
      541 ggtcctgcgc agccttaatt ttctggtgca tccactcata aagttcatca tcggtatagt
      601 ctggcgcgat gatgacgggt tctcgtttct gcatactgat tcctcgcggt gctgtttcgc
      661 ttatcagccg ttagattttg ccgaactgga aagcgcctgt ttaaactcac tgaagctgag
      721 ggcttcttcg ccttcggcaa ggccttcgaa gtattcttcg taagcctttt ccatgattgt
      781 gttgaaatcc atatcactca cctgagtttc tttccagcca gcgacgggca ccattttcgg
      841 ttttaaacgt tttgcttctg gtatacgtca tcgcggtgaa cgtaccgtcc tggttgggga
      901 acacgccaca taccagagat tcgctgttgc caagatcgat agtatccatg ctgacctcat
      961 ttccccttaa cgccggggta gcggaacaaa aacctgctgc atagttatta aagttgaacc
     1021 ctgccgtcat gttcttacgc ctcgggctgg ctacttaacc cctgaccact gccgggtaac
     1081 tcgaagtatt tccctgcgtt ctgtggggcg gggtgggttg gtatgttgtt aaggtaacaa
     1141 gagttacctt tcgagtcaat acaatgttgc aaaaggtaca tttgagggca taaaaaaccc
     1201 gcaatgaatg cgggttctga ctcagtctaa gtattgatgt atttgtgaaa ctttaccttt
     1261 aatggtgtaa ccaccattca gttcgatggg tttgtaaagc ggattcagtg acaacagata
     1321 gatgtttggt ccgtcaatcg caactttttt tagtgttacg tttggcgttc cttccaattg
     1381 gattaagatt atttttccca ccagttctct aatgttactt gagcatggtg tgatcagcac
     1441 ggtagatccg tcggggatgg ttgggaggcc gttagagttt gtcatcgcat ctccctcaac
     1501 atgcaataaa aaagagtttt cagcggtttt tgtcatgaca tcaacccagt tcttaatacc
     1561 aggaatcttg gttactggac aactcatatc ccaataacca gcctgttccc acgttaaaac
     1621 gggcaaccgg gcgatgttgt cactaatgta agggtactga ttcagacgca gatcatcggt
     1681 tttatcgtga ccgtcctttc cataaagaat ccattcagga gatttggaaa gcaattttga
     1741 cagtagatac aaattctcac cgtcaggttt tgaagagcca ttttcccatt ttgttacgga
     1801 tacacgagat atgccgattg ctttcgcaac ctgctgttgg gttaatccaa cgtcttttcg
     1861 acgattccga atacgttcgc tgatagtgtt tttcatgtaa ccaatgttac taccaagtga
     1921 tgttgctatg gttgacattg ttatgtaact attgttaccc ttctgctcga aataacagga
     1981 gagttttatg ttcaaagatg atgttctgcg ctatttcaaa aaaaagcgac tagtagctga
     2041 ggctcttgga atttcacatg tggctgttgt gcggtggaaa gcagttattc ccaaacttcg
     2101 cgcaatggaa ctggatgaaa ttactaacgg tgaattgaaa tacaacccag aactttacaa
     2161 gaagcaggat agcacctcga acgaaggaaa gaatgattca tgaaaatcaa gcatgaacac
     2221 atccgcatgg cgatgaatgt
//
LOCUS       DAZFDP010000351         2188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFDP010000351 DAZFDP010000000
VERSION     DAZFDP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..337)
                     /locus_tag="ACHJ1G_005404"
     CDS             complement(<1..337)
                     /locus_tag="ACHJ1G_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4767043.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEE"
     gene            complement(489..1088)
                     /locus_tag="ACHJ1G_005405"
     CDS             complement(489..1088)
                     /locus_tag="ACHJ1G_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4767044.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYDGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1156..>2188)
                     /locus_tag="ACHJ1G_005406"
     CDS             complement(1156..>2188)
                     /locus_tag="ACHJ1G_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4767045.1"
                     /translation="FFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQ
                     TVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAG
                     MQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFS
                     LTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFP
                     RDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKN
                     GAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMK
                     KATAGITIS"
BASE COUNT          514 a          628 c          496 g          550 t
ORIGIN      
        1 cttcttccac catcagttca aaacgacgca gtgcctccgg ccggacgtca tcctccgaca
       61 tggcaccgag aaaatcattc agcgtccccg gttgagaatc ttcatacacg gtgatggtcc
      121 cggcatgtga cggaggaaat ccctccacca acagaatgac gctgtactga ccgtactcaa
      181 cgtccatgct gtaacgtccg gcttcatccg gattttcaga ggccaccgtg ttcaccacca
      241 ccgtgctgct ggttcgtctg gccttcagca caatggtgca gttctgtact ggttttcctg
      301 tgccatcttt aagcacgcca gaaattttta ctgtcatact tttccaccaa taaaaaaagc
      361 ccgcagcagt gacgccacgg gcttcaggac agtgtaactt tacgtttcct caaacgcagt
      421 tcaccccata aggtggatga acctgcgtat cataacaata tttacagaag ataaatcggc
      481 gtctgttgtc agaaacggta tccgatacca acaataaatg catccgatcg ccagtcgcca
      541 ctaccggaac cgtcataagc aaggtcaatg gtcacggatt cggtcgggtt aaactgcacg
      601 ccagcccccc acgccagaga cgtgttgctg tggcgaccgt catcacttcc ggtcagcaca
      661 tcgtgcgttt tccccttgtt gtcagttacg cggagataat ccccggagaa agtcgacaca
      721 cggctgtaag ccacacccgc catcgcatac gcgctgaacc attcattcac gcgtacagac
      781 ggccccgcca tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg
      841 ctgtagcgcg tttttgggtc atcctcagca ttggcataac tgaaggacgt aatcagcccc
      901 agcgcgtcca taaactcata acggtatttc acgttaatcc cgttcagatc atcactaccg
      961 ggaacgttcg tcgaggcatg gagatacccc gcgctcagcg tggactgatg ttctgctgca
     1021 ctcgctggcg tagcagcggc gacctgccag actactgcgg acaaaataac agcacataat
     1081 ttacgcataa ttacctctcg cttttctgca ataaaaaagg cgccatttct ggcgcccgta
     1141 tctgggttat aaaattcagc taatcgtgat gcctgcagtg gctttcttca tcaccacaac
     1201 cagcaaatcg ctgatacttg ctgtgggata ccagttattt accagccatg ctgacaccga
     1261 aaactccagt gtcatgtgac cgtgaccggc aggcatatca ataacaccac tgtaaatcag
     1321 cgtattatcc agcgcggtac ggttataaat ttcagcaccg tttttccgca ctatcagacg
     1381 gcatgaggag taaatatcag tatgctcttt ctcatgttta gcgccgctga atgccaccgc
     1441 cggaataaca atctgccggt caaacggctg atcgtcataa accctgacgg taatggttcc
     1501 tgatggccac cgctccggtg cacgggagtc ccgggggaaa gctttgccca ctgttttaac
     1561 gagatcgcct tcaatctggt tcgcggacaa ttttcccaga acccgacagt tctcgttaat
     1621 cgtgacgttg ttgagcgtcc cggagttcgc attcacgtta ccgctgatat cggcattttt
     1681 cgccgtcagc cgcccgtccg gtgtcaggga aaatgccgga ggattaccgc cgctggtaat
     1741 ggtgggagcc gtcagatatt tcaggaacac ttcattcata aatatctgat cgccctgacc
     1801 aacaaacatc ggctttgtgt tgccattcgc aggattaatc atcgcaatcc tgtctgccgc
     1861 cagcagcacc tgactctgca ttcctgctgg cgtattctca ataccggcac cgatacccgc
     1921 aatataaagg cgtccgtcct gcatctgctg cagtttcacg gcccacatgc tgttcaggtt
     1981 attatttgta tcaacctgaa ctttctgtat ctgctggatt gccgcactct gattttccag
     2041 ttttttattg acggtctgcg tgatttcatt gctgacattc gtaatggacg tcctgatttc
     2101 agccaggtcc ggcgcaagct gaccgttatc aatctgcgtc cacagctcct gggccagatg
     2161 tgttttcccg atttctcctt tgaaaaaa
//
LOCUS       DAZFDP010000352         2166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFDP010000352 DAZFDP010000000
VERSION     DAZFDP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..268
                     /locus_tag="ACHJ1G_005407"
     CDS             62..268
                     /locus_tag="ACHJ1G_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4767046.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(524..796)
                     /locus_tag="ACHJ1G_005408"
     CDS             complement(524..796)
                     /locus_tag="ACHJ1G_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767047.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            956..1489
                     /locus_tag="ACHJ1G_005409"
     CDS             956..1489
                     /locus_tag="ACHJ1G_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4767048.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1710..1823
                     /locus_tag="ACHJ1G_005410"
     CDS             1710..1823
                     /locus_tag="ACHJ1G_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767049.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1825..2106
                     /locus_tag="ACHJ1G_005411"
                     /pseudo
     CDS             1825..2106
                     /locus_tag="ACHJ1G_005411"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2060..>2166
                     /locus_tag="ACHJ1G_005412"
     CDS             2060..>2166
                     /locus_tag="ACHJ1G_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767050.1"
                     /translation="MKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWI"
BASE COUNT          560 a          434 c          591 g          581 t
ORIGIN      
        1 gcggcttttt tgtttttctt tactttcatt ttctgtcggc ggtgacggag acatacatca
       61 gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggga cgggatactg
      121 gttactgcag ctgctggaca aagtctctcc gtcccagtgg gtggcaatag gtgtgctggg
      181 aagtctgctg tttggcctgc tgacgtatct gactaacctg tatttcaaaa tcagagagga
      241 ccgtcgtaag gtggcgcggg gagagtagtc gatgaataaa caatacgaac tggttgtaaa
      301 atgaatattt ctaactgaaa aaacgttcca tgaggtaaga aaaggtcaca ggcaatcaat
      361 aacaggacgt gatgaaagac ccttgcattt gtgcgctttc tctttagata gcagcagata
      421 ctgaaaatct gagttgtcgg ggagtcaggg atacagctgt gcaagagttg gtcattgtga
      481 ttccattgaa atcctgtatg ccatgaaggg caggatttta tggctacctg agctttggtg
      541 atagtaagtt gaaaattcgc attttttgct gacatgcgta acgagaatcc cataagcagg
      601 gaggacttaa ttcttcatta acccatgcgt tgatattatg tttcagccgt tgaagcatca
      661 gcggtgttaa tgttgtggta ataatatcca gcgttttatg tgagatctta ccgtaagggt
      721 ctgcaagaat gctgcttgtt gcttcgttat tatctgccat cagaagaagt aactctgatt
      781 taacgttttc tgtcattagt tgtaaaaatc ttctgcgcaa actttcttta ctgttcattt
      841 atatggcttc atttgttgta atctgctgcg tctcaaggga tatgtttatg agagcgacca
      901 tgagtgttgg attatatatc taacatatca agggattaga aatcgataaa tccccatgaa
      961 cgaaaaaata aaatacggcc tgtcggctgc cgttctggcg ctgattggtg caggtgcttc
     1021 tgcgcctgaa atcctcgacc agtttctgga tgaaaaggaa ggtaaccaca ccacggcata
     1081 ccgtgatggt gcggggatct ggaccatctg ccgtggtgcc attctggtgg atggtaagcc
     1141 tgttattcct ggcatgaagc tgtcaaagga aaaatgcgac cgggttaatg ccatcgaacg
     1201 tgacaaggcg ctggcatggg tggagaaaaa catcaaagtg ccgctgaccg aaccccagaa
     1261 agcggggatc gcgtcattct gtccgtataa cattggcccc ggtaagtgtt tcccgtcgac
     1321 gttttataaa cgaattaatg caggtgatcg aaaaggtgcc tgcgaagcga ttcgctggtg
     1381 gattaaggac ggtggcagag actgccgtat ccgttcaaat aattgctacg gtcaggtctc
     1441 acggcgtgac caggagagcg cgctggcgtg ctgggacatc gacagatagc agaatatttt
     1501 cctgaaaaat gacgttggcc aacgcgggtg gataacacga aatcctgaaa actggtaaaa
     1561 cctaagtgaa taaaagtaaa aaccccgttt gttggcagca agcggggttt tgtgttttct
     1621 gaccttgagt aaggcaaggg agaaattatg ggtagggagg tactttccct gtgaggaagt
     1681 ataaaagatt ctttctgagg ttgtccatta tgaaaggcat tgaagtggag acgccagcca
     1741 gtctggattt aacaagagcg gcagcttttg ccattcgtat tgtggccatt gctgttctgg
     1801 tctgggcaat ccgttggtgg tgatatgaac cgtgttctgt gcgtggttat cattgtcctg
     1861 ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt accgtgatca cgccatcatc
     1921 tacaaagagc agcgcgataa aaaagccagt gagctggagc tggcgaacgc gacaattact
     1981 gatatgcaga tacgccagcg tgatgtcgct gcacttgatg ccagatactc gagggaatta
     2041 gccgatgcga gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc
     2101 ctgcggatca acgccacctg ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg
     2161 gataat
//
LOCUS       DAZFDP010000353         2158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFDP010000353 DAZFDP010000000
VERSION     DAZFDP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            277..1254
                     /locus_tag="ACHJ1G_005413"
                     /pseudo
     CDS             277..1254
                     /locus_tag="ACHJ1G_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1777..>2158
                     /locus_tag="ACHJ1G_005414"
     CDS             1777..>2158
                     /locus_tag="ACHJ1G_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767051.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGI"
BASE COUNT          661 a          468 c          483 g          546 t
ORIGIN      
        1 tttacaatga agaattattg ttaaaaaacg ctttgttgaa tcaaccgaac tgttgctgag
       61 ttgcaggatc acatcacgta ttttcctgaa aacgcaggct gcttagtagc aaagcagaag
      121 ttcaaaatca ccaactaaat agctgcgccg aatagtagat caatgaaagg gaactcagcc
      181 cggattgtgc gatctgatca atcgccaaaa atccccccct aaccggactg agcgatgccg
      241 atcatagcac caattccccg cgacgaatga cgcctgatgc aaaaagcaat ctataaaact
      301 cacgataaaa attatgcccg caggctgacc gccatgctga tgctgcaccg gagcgctcgc
      361 gtcagtgacg ttgccagaac gctctgctgc gcccgttcct ccgtcaggcg ctggattaac
      421 tggttcacgc tgtcgagtgt tgaaggtctg aaatcattac ccgcaggacg ttcccgccgc
      481 tggccgtttg agcatatctg cacattgttt cgtgaactga taaaacactc tcccggatat
      541 tttggctatc agcgttcacg ctggagtaca gaactgctgg caataaaaat caacgagata
      601 accgagtgcc agttacatgc cggaaccgtt cgccgctggt tgccattagt cgggcttgtg
      661 tggcgcaggg ctgcgccaac cctgcgtacc cgtaacccgc ataaagcagc aattcacaaa
      721 gcactggacg aatgtagtgc ggagcatccg gtcttttatg aagatgaagt gtatatctat
      781 cttaatccca aaaccggtgc ggactggcaa cttcgcagac agcaaaaaca tgtggtaaca
      841 ccggggcaga atgaaaaata ctatctggct ggtgctttac atagtggaac gggcaaagtc
      901 agctatgtgg gtggcaacag caaaagctca gccttattta tcagtctgtt gaaacatctg
      961 aaattgacat accgacggga taaacctatc acgctcatag tggacaacta catcatccat
     1021 aaaagtcggg aaacacagcg ctggctgaaa gaaaatccga agttcagagt gatttatcag
     1081 ccagtttact cgccgtgggt gaatcatgtt gagcggttat agcaggcgct tcacgatacg
     1141 ataacgcgca atcatcagtg ccgttcaatg tggtaactgc ttaaaaaggt tcattatttt
     1201 atggaaaccg tgagcccatt ccccggtggt aaatatgggc tgttaaaagt gtagcggtat
     1261 tcggcgcagc tatttaggat ggtcaggtcg cagaagttct gtgccactac agtgcgtgta
     1321 ctaaacaaaa tgacgagggc acgaatgtca taatacgtgc gtattacctg cagtagctac
     1381 caactgaggg ggcttatccc aaacctgatt tattcaacaa agctggactg gacttatcct
     1441 tactctgaat tattttccag acatatgcat catgtactgg ctcgcttggt tcaggaaata
     1501 caggagcaaa tctacagcta ttagatttat tcctatttgt atgcaaagtc gcattgcata
     1561 atgcggagct atgttacaga tacgcctgac gtgcgtagta aaattataaa gcaacatata
     1621 tatacaagga gaaaccgaga aaaataaatg agtcgtatgt tgggtagcat cgagataaat
     1681 atttttttat cacattagca ccttggttaa gggtgttaat aataagattt tctcacttat
     1741 taaaacataa ataatttttt aagcggaaat gtaattatga gactgccaga gaaagttctt
     1801 tttcctcctg tcactagtgg cctgtcaggg caggaaaaac aaaaaaaacc gaagagcatt
     1861 accggatttc aggaaaatta tcaacgcaat atcaggccaa tcaaaacagc atcagaagcc
     1921 cgactacgct tctttgataa aatggtttcg aaagaaaact ctctggaaga tgttgtttct
     1981 ttaggtgaaa tgattcagaa ggaaatttat gggcatgaac aaagaacatt ttcaccagtt
     2041 catcatacag gtaactggaa atcatcattg ttacacaacg cgctccttgg tctggcaaat
     2101 gtttataatg gcttacggga aacagaatac cctaacactt tcaacagaga tggtataa
//
LOCUS       DAZFDP010000354         2109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFDP010000354 DAZFDP010000000
VERSION     DAZFDP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..84)
                     /locus_tag="ACHJ1G_005415"
     CDS             complement(<1..84)
                     /locus_tag="ACHJ1G_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767052.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGE"
     gene            complement(371..1189)
                     /locus_tag="ACHJ1G_005416"
     CDS             complement(371..1189)
                     /locus_tag="ACHJ1G_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO4767053.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1341..1712)
                     /locus_tag="ACHJ1G_005417"
     CDS             complement(1341..1712)
                     /locus_tag="ACHJ1G_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4767054.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1702..2073)
                     /locus_tag="ACHJ1G_005418"
     CDS             complement(1702..2073)
                     /locus_tag="ACHJ1G_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4767055.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
BASE COUNT          710 a          535 c          347 g          517 t
ORIGIN      
        1 ctccccgact gtcgccggtt cggtgacgct taattcatca aacacaactc tggcggtttc
       61 tgtcatatcc tgctgtttca gcatgtcttt ttaccctttc cggttaacgt gacacaccaa
      121 taactcttgc cgaaaaagcc agcaagctga aagacctgta ttaataacta ccagcacatt
      181 taacgcactg cgctactttg cgggcacaaa aaacccgctc agaggcgggg tcaagctatg
      241 cggcgaaata accactctta acagcatacc tgatttttta cgtacgtaaa tgctttgccg
      301 tgcatatttt tcatgcaaat gtcacgtcct gctatttttc agtcttataa atttaaaacc
      361 atagaaaaaa tcaattatgt tttaaaaatg gataggtaaa gaataacaag tgacacagat
      421 tcaaaccaaa atggaaaagg gtggcaaccc acaaacgccc actccacatc catgacaatc
      481 catacacaac accagataac gtggcaaata atacaagtaa agcaccacct gaatagtgat
      541 aaaaaccaaa caacaaagcc gccacaatta atgcaaccaa tggagacgtt acttctgata
      601 gccgtgattg aatataccct ctaaataatg attcctctgc cagagacaca aaaaacaaat
      661 tagccaatat aaactctggc aaccactcag gaaaatgaat ctctggcttt aatccaccaa
      721 aaaaaacagc caaaaacagg ataagaggaa cagagagcga cagagccccc cacttccaca
      781 aagacacttc tgattttact tcttttttaa acaaagaaga tgtacacagg actaacaaaa
      841 atggcaccag cgctttatca aaattaaaat acattgtata gggagtactc tgagggccaa
      901 cagtaacaga atttagcact acaggattgt gaaaccctgg ccatagatgg aaagataatg
      961 ctatggctga taaaactatg ccaacttcat atatagattt agcccaggcg ttatatttcc
     1021 agttgaactt caaaataata aaaaaaacga ttgtagcaac aaaaaataac accgaccaat
     1081 caataatatc attaagcaca gccaggacaa cagacaccgt caacaatgaa aaagccacta
     1141 ccttatgcca actgagagtt gacagtgaca gtactaacac aatccacata agcactcctt
     1201 ttatttaatg atgaagattg attatcaata ttttcaattc accaggcaac attttatcta
     1261 ccttccacaa tacatgacca tcaaaaaata aacatgttaa ttataaacac agaaaacata
     1321 accctcatca ctatatatcc ctaccgcata tccatatcta accggacatt cagagccata
     1381 agcattcctt caataatacc ttccgcttta taaagccttt taccaataag cccatcagaa
     1441 catctatgct tacgtgcaag agccataaat gtcattccac ctacgtaata atccaccaac
     1501 aaatcgtgca aatactgatt attcttgttt aatcgtgcca tacaaccaca aattatcatg
     1561 gcgtcatcat cagaacactg aggacgtgat ttcactttag tcgggattaa tcctttaaaa
     1621 ccagcagcta tcgagggcca tgttacatct tcatgattat ttgctgccca tgccccccat
     1681 cgctcaagaa ccatctggat atcacgcgcc atcgttacca cctctgattt cgtaaatctt
     1741 cacgcccagc cgtccacctg gcacaggctg accgcgcaca atattgattt catcaaactg
     1801 ctcgtcatca atgagcactt ccgcatgcgt cagcgcatcc agcggtgctt tcagaatgtt
     1861 gtccaggtca cggcggcgct tatccggtgg ttctgcaata atttttattg ccagccgtcc
     1921 ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc ggcgataacg
     1981 ctcaccggct tttgatacaa aatatgtgct gccacgacgt cgccagtagg tgttcaccgt
     2041 cggcgggtaa ggcaaaacaa actctatacg catcagtaac ctcttttacc cgagcacgcc
     2101 ggttgcaaa
//
LOCUS       DAZFDP010000355         2100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFDP010000355 DAZFDP010000000
VERSION     DAZFDP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..507
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005419"
     CDS             160..507
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4767056.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKAFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            556..2094
                     /locus_tag="ACHJ1G_005420"
     CDS             556..2094
                     /locus_tag="ACHJ1G_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO4767057.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          473 a          604 c          624 g          399 t
ORIGIN      
        1 tgttgtccct acaacctccc cccggtcaca acctgtagct gctgcgacac ctgaatcact
       61 caatatcagc tgtgaagtga cgttccggca cggatcactc cgtctgaatg gtgccatcag
      121 cgaaaatatc ctgaacctgc tgatacggga gctcaaacgt tgatcccatt accatcaggg
      181 acaaagatct ggctggtcgc tggcatcacc gatatgagaa acggcttcaa cggcctggcg
      241 gcaaaggtgc agacgacgct gaaagacgat ccgatgtcag gtcacgtttt tatcttccgt
      301 gggcgtaatg gcagtcaggt aaagctcctc tggtctaccg gcgatggact gtgtctgctg
      361 accaaacggc tggagcgcgg ccgcttcgcc tggccgtcag cccgggatgg caaagcgttc
      421 ctcacaccgg cacagctggc gatgctgctg gaaggtatcg actggcggca gcctaaaaga
      481 ctgcttacgt ccctgactat gttgtaagcc tctttatcct ggtcgacgct gaatgagcct
      541 ggtaatatac ccggtatgag cagctcactt cctgacgata tcaatgcact gaaacgtctc
      601 cttgccgaac aggaggcgct gaaccgtgcc ctgctggaaa agctgaacga gcgtgaacgc
      661 gaaatagacc atctgcaggc acagctggat aagctgcgcc ggatgaactt cggcagccgc
      721 tccgaaaaag tctcccgtcg tatcgcacag atggaagctg acctgaaggc acttcagaaa
      781 gaaagtgata cccttaccgg tcgggttgac gacccggccg tgcagcgccc gctgcgtcaa
      841 acccgcaccc gcaaaccgtt ccccgaatca ctcccccgcg atgaaaaacg gctgctgccg
      901 gcagcatcat gctgcccgga atgtggaggc tcactgagct atctgggtga ggatgccgcc
      961 gaacagctgg agttgatgcg cagcgccttc cgggttatcc ggactgtacg tgaaaagcat
     1021 gcctgtactc agtgcgatgc catcgtgcag gcccccgcgc cttcacggcc catcgagcgg
     1081 ggtatcgcag gaccggggct gctggcccgc gtgctgatct caaagtatgc agagcacacc
     1141 ccgctgtacc gccagtctga aatgtacggc cgccagggcg tggagctgag tcgttcactg
     1201 ctgtcgggct gggtggatgc atgctgccgg ctactgtcac cgctggaaga agcgcttcag
     1261 gactatgtgc tgactgacgg taagctccat gctgatgaca cgcctgtccc ggtgctgttg
     1321 ccaggcaata agaaaacgaa gaccgggcgg ttatggacct acgttcgtga cgaccgtaac
     1381 gccgggtcaa cgctggcgcc ggcggtgttg ttcgcttaca gcccggacag aaaaggcatc
     1441 catccgcaga cccatcttgc ggggttcagt ggtgtactgc aggcggatgc atacgccggg
     1501 ttcaacgagc tgtaccggga tggccggata acggaagccg cctgttgggc tcacgcccgc
     1561 cgtaaaatcc acgatgtgca cgttcgcacc ccgtcagccc tgacggagga agcgctgaaa
     1621 cggatcggcg aactgtacgc catcgaggca gagataaggg gaatgacggc ggagcagcgc
     1681 cttgccgaac gtcagttgaa aacgaaaccg ctgctgaaat ccctggaaag ctggctgcgt
     1741 gaaaagatga aaaccctgtc gcgacactca gaactggcga aagcgttcgc atacgccctg
     1801 aaccagtggc cggcgctgac gtactatgca gatgatggct gggctgaggc ggacaataac
     1861 atcgctgaaa atgcgttgcg gatggtcagt ctgggccgca aaaactacct gttcttcggt
     1921 tcggatcatg gaggagagcg gggagcgctg ctgtacagcc tgatcgggac gtgcaaactg
     1981 aacggagtgg agccagaaag ctacctccgc tatgtccttg acgtcatagc cgactggccg
     2041 ataaaccggg tcggcgaact gctcccctgg cgcgtagcac tgccgactga ataacacatc
//
LOCUS       DAZFDP010000356         2080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFDP010000356 DAZFDP010000000
VERSION     DAZFDP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..659
                     /locus_tag="ACHJ1G_005421"
     CDS             <1..659
                     /locus_tag="ACHJ1G_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4767058.1"
                     /translation="EEWREWQLAEGRKRSEEINRLNHQVRVEKILNRAGIQPLHRKCS
                     FGNYRVQNDGQRHALSQAKSIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGR
                     SVIIVTVSDVMSVLHDGYDNGQSGEKFLQELCGVDLLVLDEIGMQRDTRNEQVTLNQI
                     VDRRTASMRSVGMLTNLNHAAMSTLLGDRVMDRMTMNGGRWVNFNWESWRSNVGRQGM
                     "
     gene            685..1446
                     /locus_tag="ACHJ1G_005422"
     CDS             685..1446
                     /locus_tag="ACHJ1G_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4767059.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            1479..1760
                     /locus_tag="ACHJ1G_005423"
     CDS             1479..1760
                     /locus_tag="ACHJ1G_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4767060.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1757..1984
                     /locus_tag="ACHJ1G_005424"
     CDS             1757..1984
                     /locus_tag="ACHJ1G_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767061.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1977..>2080
                     /locus_tag="ACHJ1G_005425"
     CDS             1977..>2080
                     /locus_tag="ACHJ1G_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767062.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYIST"
BASE COUNT          546 a          428 c          662 g          444 t
ORIGIN      
        1 tggaggagtg gcgggaatgg caacttgctg aaggacgaaa acgcagcgag gagattaacc
       61 gcctgaatca tcaggtgcgg gttgaaaaaa tcctgaaccg tgcgggcatc cagccgcttc
      121 acaggaagtg ctcattcggg aactaccggg tgcagaacga cggtcagcgc catgctctga
      181 gccaggcgaa atccattgcc gatgaattga tgaccggatg tacaaacttc gtgttcagcg
      241 gtaaacctgg taccggtaaa aatcacctgg cagcagcgat tggcaatcgg ctgatggcga
      301 aggggagaag cgtgattatc gtcaccgtgt ccgatgtcat gagcgtgttg catgacggct
      361 acgacaacgg ccagtccggg gaaaaatttt tacaggagct ttgtggagtt gaccttctgg
      421 tccttgatga aattggcatg cagcgggata cgcgcaacga gcaggtcacg ctgaaccaga
      481 tagtcgaccg cagaacggct tcgatgcgta gtgtcggaat gctgacgaac ctgaatcacg
      541 cagcgatgag cacactcctc ggagatcggg tgatggaccg tatgaccatg aatggtggtc
      601 gttgggtgaa ttttaactgg gagagctggc ggtcaaacgt tggacgtcag ggtatgtgag
      661 aatttttgac gaggtaaatt ttcgatggaa actgtattgc atgcactgaa agcgatggga
      721 aaagccaatt ctgttgaact ggcggcgcgg cttgatatca gccgtgaaga agttcttaac
      781 gaactgtggg aactcaaaaa aaatggcgtt gttgataaaa cgggtcacac ctggtttctg
      841 gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac tacaacctga agcgccggat
      901 gtggtaaccg aagaggtcgc tccaaaagtt accgcagaca tgatggttga gtttatcggt
      961 caggatggtg ctaaaacgtg tgaggaactg gcgggtaagt tcggcgtcag tactcgcaag
     1021 gttgcttcca cgctggcggt ggtaaccgca acggggcggc tggcacgcgt taatcagaac
     1081 ggtaaatttc gttactgcat gtcggggggt aatttaccag cagatccgaa agccgcgccg
     1141 gtaacgaaaa atgatggtaa ggcctttcct cagccagcag gtgctgcgtt accagtccgg
     1201 gaagccgcaa cacaggaaga aataaaaacg gaaagtgtgg cggtcacagt gcagtcacag
     1261 ccgtcgttca ccagaaagca tccggatggt ctgattttac catcgctgca tgtggctaac
     1321 cgcgagctgc gccgggcaaa aggtcaggtt cagaagtggg agcgagtctg cgccgcgctg
     1381 cgggagctga acaagtgccg ggatattctc cgggatatta ccgccaccag agaacagcag
     1441 cggtgagtgg gtggaagacg tggtgccggg cggaaatcat gatactccgg cagtgtgcgg
     1501 gaacgatgaa ggtaaaaagc gttggcgcac ttatcggacg aactgaagcg gcagtgagaa
     1561 cgaaggcacg ggagctgggc atcagcatga tgttacgtgg tgattttcac ccgtcggcaa
     1621 aatattctca gcgtgatatt gagctggcgc ggcaactgca tcagagaggc atgcaaagaa
     1681 gggaaattgc cagaaaatta ggcatgccgc tgcgcatagt gaataactac gtttatttcg
     1741 acaggagggt gtctgcgtga aaatcctgta tcaggattac ggcccggtgg ggcaggtggt
     1801 tatcagcagt actgtaatgg agtttcggaa gcataaccgt gtggtggatg ctgtgctgtt
     1861 aacctgtccg gggatatcgg cgagtcgtgc aggtgtgttt attatgaaga cgaaattata
     1921 tggcagtaag gcgtggataa agaaggcgta tcgtgtagcg ttgcaggagg ttaacagtga
     1981 gtgaaattaa agaaatgccg gtagttcgtg acggatatgg ctactggaca catcctgaat
     2041 atgaaaaatt ctgtgatggt cgggaatata tttcaacgga
//
LOCUS       DAZFDP010000357         2070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFDP010000357 DAZFDP010000000
VERSION     DAZFDP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..275
                     /locus_tag="ACHJ1G_005426"
     CDS             6..275
                     /locus_tag="ACHJ1G_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4767063.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHJ1G_005427"
     CDS             complement(392..667)
                     /locus_tag="ACHJ1G_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4767064.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..>2070)
                     /gene="espK"
                     /locus_tag="ACHJ1G_005428"
     CDS             complement(730..>2070)
                     /gene="espK"
                     /locus_tag="ACHJ1G_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO4767065.1"
                     /translation="RPTGTFCSYSAETSAGIKSEITPIQIEEARASGRLYIKDCDIEY
                     LPQLPNEITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKNLHIELTD
                     SPFIHIIPEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSLSINSYNP
                     ENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIGVEGMPDG
                     LDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVNSIKLVND
                     FSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSHNNYNVTM
                     ADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDGYGQSITA
                     SELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATYKYS"
BASE COUNT          479 a          395 c          494 g          702 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacggaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 aggtatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt ttactgtcga
     1801 tggtatgtct gtgatttgta atttttcaca gttaatgacg gagaggtttt gtgtattaac
     1861 cggcaatcct gtaaggattg tcaggttgtt gcagttttcg attgtaactg atgttatttc
     1921 gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac gaccactggc
     1981 ccgcgcttct tctatctgaa ttggtgtgat ttcgcttttg atgcctgctg atgtttcagc
     2041 ggaataggag cagaatgtcc cggtcggtcg
//
LOCUS       DAZFDP010000358         2027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFDP010000358 DAZFDP010000000
VERSION     DAZFDP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            181..1413
                     /locus_tag="ACHJ1G_005429"
     CDS             181..1413
                     /locus_tag="ACHJ1G_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4767066.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPEALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1416..>2027
                     /locus_tag="ACHJ1G_005430"
     CDS             1416..>2027
                     /locus_tag="ACHJ1G_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO4767067.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWA"
BASE COUNT          617 a          374 c          406 g          630 t
ORIGIN      
        1 aaaatatttt tttcctcaac aaattactgc aaatgagata tgcatcacta tttgcagtta
       61 ttcatcatta aaatcttatt tgacagttga ttggccttga tttgtgttga taatggcgtg
      121 aatgcttatg taggcgtaca gcatgtacta cttaaagata ttattcacga ggtaaatata
      181 atgagacttt caaatatcgt cacagttata attcttgcaa tttccagtac gctaacaccc
      241 caggcgatgg cagaaaaatt gattcctgaa actgatgctg agttattaat ctggtctgat
      301 gccacaagcg cggaatatat gaaatatgca gcaacggaat tcaataaaga ttttggctac
      361 aaggtcaagt ttacatttcg caatatagcg ccaatggatg cagcatcaag aattatgcag
      421 gatgggggta cgactcgtgt agctgatgta gctgaaattg aacatgatac cctggggcgg
      481 ttagtcgttg ctggcggggt tatggaaaac atggtctcag ctgagcggat taaaaaaaca
      541 tttattccag gcgcagtatc ggcagctaca tataataaca tcagctatgg ttttcctgta
      601 agtttcgcaa cgctggcgct tttctataat aaggatttgt taaacaccgc accaaaaaca
      661 ttcgaagaaa tcaatacttt cagtgaaaag tttaataatt catccgagca taaatatgct
      721 ctgctatggg atgtacaaaa ttattatgtt tcacgtatgt ttattacctt gtatggtgcc
      781 aacgaattcg gtaaaaccgg taacgatcct gaagctctag gcatcgcttc atctgaagcg
      841 aagaaagggc tagagacaat gaaacgctta aagaaagcga atccttctaa tcctcttgat
      901 atgggtaatc cacaagttct aagaggtctg tttaatgaag gaaaagttgc tgctgtaatc
      961 gacggacctt ggtccataca aggttacatt gacagcggaa tcaattttgg cgtgacacgc
     1021 atcccaacat tagatggtca tcagcctcgc acattttcaa cagtacgact ggccgttgta
     1081 agctcattta ccgaatatcc tcatgccgct gaattattcg ctgactacct gacaacggat
     1141 aagatgttga tgaagcgtta tgaaatgacg aatctcatcc caccaatcga ttcacttatg
     1201 aacaaaatta gccagacagg tagtgaagct ataaaagcaa ttattgctca agctaattat
     1261 tctgacgcaa tgccatcaat accggaaatg tcttatctat ggtctccaat gactaatgct
     1321 attttggcta cctgggttga gaataaaaca ccggatgaag ttttaaatca tgcacaaaca
     1381 attattgaag aacaactttc gcttcaggag taattgtgaa tacacaacaa ctgaaaatga
     1441 agagtgcacc tgtgctacct atttcctgtt tgattatggg cgggactcag ttatcacgtc
     1501 attattatgt gaaaggcggg atattctttg caatacaagt atgctttttg ctttatcttt
     1561 ccgatattgt gcatacatta ataggtttgt tcacattagg tgatgtcgcg caaattcgaa
     1621 aagggttgac tgttattcag ggtgacaatt ctatatttat gcttgtagag ggcgtgatag
     1681 cgaccattat tgttggctta ttcgccacta tttacatcct taatattaaa gatgcgcgga
     1741 actcatctta ttgccatctg acttttaaac aacagttata taagctctat gaggataaat
     1801 ttgcatttat agtcctaacg cccgcatttt tagcgagtat agcattcatt gttttaccta
     1861 ttgtaattac agttcttgta tcgtttacaa actatgctgc gccaaatcac atcccaccaa
     1921 agaacctggt tgattgggtt gggattaaaa atttcattat gttgtttaaa tttaagatat
     1981 ggtctgatac gtttctaggt gtcgctctct ggacatttat ttgggca
//
LOCUS       DAZFDP010000359         2017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFDP010000359 DAZFDP010000000
VERSION     DAZFDP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1830)
                     /locus_tag="ACHJ1G_005431"
     CDS             complement(<1..1830)
                     /locus_tag="ACHJ1G_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO4767068.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQT"
BASE COUNT          635 a          389 c          355 g          638 t
ORIGIN      
        1 ggtttgacca aatggtataa gttttctgac tattttagtc ttgtttaaat aaaaacagcc
       61 ttcaatgtat tgtttgccct ctggtgaggt tgaatatgaa aaagattcca tatcagcata
      121 agagaactgg atagattcat cagctctact acagggtatg tatcctgagt caaaatattt
      181 cccgttcagc agagtttttt tgattaaaat atcgctctca agctcaattg catataaaac
      241 gggacctctg gaaatataag tgtcaccaca taagtcagta ttaaactgta tatcagctat
      301 aaatttaatg tcaatcacat catgatgttg ccatgttcta tctaaaataa taagtgagtt
      361 ttgagtatca tttttagtaa aagtaacacc attaataatc atcatttttg cccagccggg
      421 gtaacggaaa ctgagagcaa aacgaacaga tgactcaggc tcaatgagac attttacgct
      481 taaatcatga gggtattcag ttaattgtgt aattttaatt gcaacattat taaatgtgcc
      541 ataaaactca cacgggccat aaaataatgc agtaaaacca ttgttatttt cttgaaacat
      601 attttcgaca aaagccggta tggttcttgc tgtattcggt acgcagcaga cagcagcctc
      661 ctcatgagta ggggagtatt tatatctggt attaaactga ttactgtccg gatgtttcct
      721 ttcatttgct tcataacaat tatcagtctt acagtacatt atcgcgtgaa gtatgggatg
      781 ctgcatgcca aaactagcat tatagaatag ccactcagca tcatcggcca gaccaatatt
      841 agtggtcagt tttattaaca taacataagt gctaaatagt tcatgaacag aacaatattc
      901 atagcctgta taagtggcat tagctgtgcg tcctgtaatc cactcatcac caatgggagc
      961 gccagatggt gtaagataat atttcaactg ggataaaaaa atatctaatg cagtttgata
     1021 ctgcggccgt tggctcgctg cgatggctaa tgccctaata tgctcatacg tatgtacgcc
     1081 atgaccttta agaaggtaac cagggtctat gacattttta tattgcagat cttgctccga
     1141 cacgcatcct gcggaataat cttcatacag ccacaagcaa tatgagagat attcaatgtt
     1201 attggttatt tcataaaggc ggtagaaaac atctgttatt gttaatccgt ggctatgtcc
     1261 agcaaaaccc tgttccacaa cgaaaggatg tgacgtttct ttagggtagc cagccataat
     1321 gcattgtgcg gcttgggtaa gagaagttaa taaactctca ttacgggtgg actcataaca
     1381 tccaagcaca aaacgcaagg cagtagcttg tgcccagaac tctccatttt ctgctttaca
     1441 gttaaatttt aatgatgaat cataaattcc aatatagcca tcatcatctt gcgtatcgat
     1501 aagttccaaa cataaatcgc tcgctttttc ctgccattct ttattatcta acaagtaggc
     1561 actgcggcaa tatccatcct tccaattcga ttgagtttca ctatcccacc aataatattg
     1621 catcatgtta tcatcgggat tattctgttt tttaataacg ccatcttcag cagatacagt
     1681 gcgtggagtt atacgatctc ttccatatat tttatgctcc gtaataagat ggggaactaa
     1741 tttatccagt tctcctatat gaccgttcat ataccattcc atcttattct ttaaccaacc
     1801 agtgggcttt atatcgttaa aaaaaatcat aaatatacct ttgcataaaa aagtttagac
     1861 gttataaaag acaaatagat tgtgatcgcc tacagacgat cacaatgaaa ggaaaatatt
     1921 aacggttttt gctatcactc aaatgttttt catcctgcac catatcattg ccagattcat
     1981 gcaaagtatt tattaatgca ttggcatctg gatattg
//
LOCUS       DAZFDP010000360         2006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFDP010000360 DAZFDP010000000
VERSION     DAZFDP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..905
                     /locus_tag="ACHJ1G_005432"
     CDS             153..905
                     /locus_tag="ACHJ1G_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO4767069.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            902..1456
                     /locus_tag="ACHJ1G_005433"
     CDS             902..1456
                     /locus_tag="ACHJ1G_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4767070.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1458..1730
                     /locus_tag="ACHJ1G_005434"
     CDS             1458..1730
                     /locus_tag="ACHJ1G_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4767071.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          571 a          493 c          499 g          443 t
ORIGIN      
        1 tattaaagga agcgtgtaga tcagacatga cacctttctc tggctagcca gaacaaggtc
       61 attgttcagt cactgacaca atcctcctcg gtagtgcgct tgtatggacc ccggcacaat
      121 tgacacaaca acaaaacact accggagcag tcatgactac tgttaaccag atccctgatg
      181 caaacattac accctccctc ccaccgaaaa ttcgcgaagc ggtggataaa gttaaggcag
      241 caaaagctgt ctggcaggaa gaacgacgaa aacaaactga agctgctgca atgattgaaa
      301 ctatccgtaa acgtcaggaa gatacaaaaa cggaaacgca ggcgcttaat gatgaatggc
      361 gaaacctgtt tcgtgagaat cagggaaata tgacgccacg catgaaaaaa ctgcgggcag
      421 aaatcgccct cggacgcgaa acactggatg agttcgaaga tttgctggca gctcaggcgg
      481 cagaaaatga attcctgccc tggaaaactg cggatgctgc aaaccactac atcagcgaac
      541 ataatcgcct gattgaaact catgcagtgt ggctctggaa tgagtttatg aaggaacacg
      601 gtcagaaact tattcagatc cttggactgc tgaaaatgac tctgggccga agcgcttctg
      661 ctgttatcgg tgtagttcat accgtaaacg accccgaaag tgtgctgaag caatttatca
      721 gcgagcaact caccactccg gcactgtcct gtaacgtatc ttcaaccgat gatattaccc
      781 tgccggggat cagcatttat gcggacgata aagccatcca ggatgccaga caatccccca
      841 gccctgcagc gcgttcacgg atgcttaaac agcgtgacat ggttaaaggg ggcgagaaga
      901 aatgaatgcc ggaaccatta ctcaggaagc actcaacgat taccgctcgg cgataaaaag
      961 ctggctgaca ctacgtaata cgcaaagtac ctaccagctt cgtctggcgg ctctgctgga
     1021 tactgaagaa aaaccggcag catatgccag ccagcttgag gatcttcgtg agcgtcttgc
     1081 agtcctcgaa tggcagatta actgtgctgc acgggatggt ctttatgctc atcagattgt
     1141 gctggagagt tgtgttacaa gcgcaacgga aaacttcatg agcgagcatg gtgatgcact
     1201 cactgacgct ctggctcctt ttctttgcgc tccatacggg cttgaggcgg caatgaaaat
     1261 attacgaacc gctgtagccc gacaaacgga aatgcgtact ccggtaattt cagcagcata
     1321 taagagcatt atcgacgaaa ccggattaac ggtggatgca tcaatgcgcg ctgatgcttc
     1381 agccagtttc accccggcac aacataaagt tttcctggcc cgccttaacc gacttaatga
     1441 aaaaggagtg tgctgatatg gccctgaagt gtcctgaatg tggcgcggtc gctcacgcca
     1501 gaaccagcgc ctatgaagct ccgtcggtta aacgctcatg gtatcagtgc cagaatctgg
     1561 aatgttcctg cacatttacc gcactggaaa gcgtggatac gataattatg aagcctcggt
     1621 gcaatgaaca ggaatcagac aaagcaaaaa taccggaaaa acaacagcaa actctcaatc
     1681 gctatggctc cgcgtcaaag ctgtcaaacc gtcagcaaat tcctgtctga ttagcaaaat
     1741 acgaccacga agtcccggtc cagtaccggg attttttacg cctttttcct ggctggcctg
     1801 agagcgtatg agtgcatatc tatggcgcat gaaaacgcat gagtctcatg cgccattttt
     1861 gacgcgaaag cccttgtgtg gtggcttctg agacgattta cggggtgcat gaaaaccagt
     1921 ctgttaagcg aagcgggcag gcgggcgggg cattgcgcgc aggaatgtgt ataccgtttt
     1981 agtaatgatc gctgacatct cacttt
//
LOCUS       DAZFDP010000361         1968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFDP010000361 DAZFDP010000000
VERSION     DAZFDP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /locus_tag="ACHJ1G_005435"
     CDS             <1..183
                     /locus_tag="ACHJ1G_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4767072.1"
                     /translation="PTRPVTNPFWRNKPDECPECDPGNVPRCELHLKSGVVKLFDPLT
                     PEMLRALIREMKGDTR"
     gene            180..344
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005436"
                     /pseudo
     CDS             180..344
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            493..1512
                     /locus_tag="ACHJ1G_005437"
     CDS             493..1512
                     /locus_tag="ACHJ1G_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFO4767073.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            1787..1968
                     /locus_tag="ACHJ1G_005438"
                     /pseudo
     CDS             1787..1968
                     /locus_tag="ACHJ1G_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          553 a          447 c          543 g          425 t
ORIGIN      
        1 ccgaccagac cagttacaaa tcccttctgg cgtaacaagc ctgatgagtg ccctgagtgt
       61 gatcccggaa acgtcccacg gtgcgagctg catcttaaat caggtgtggt aaaactgttt
      121 gaccctctca ctccggaaat gttacgggcg ctaatccgcg aaatgaaagg agatacccga
      181 tgataacgct gccaaccggt accagaatct ggatcatcgc tggcatcaca gatatgcgtt
      241 gtggcttcaa cggcctggct tcgaaggtgc agaacacgct gaaagatgac ccgttctccg
      301 ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt gaaaatgtaa tgaacgcatc
      361 ccacacaatt aaacacggag cagtatgatg gttaaaaggg tgaaactggc aggtgtggag
      421 cctcgcagat ttccggcatc atagtgccca atacgggatg cataaagctc cctcagactg
      481 gagactccac aaatgaaata tacaccggtt ggcgttgata tcgcaaaaca tgtcattcag
      541 attcacttca tcaatgagca cacaggtgaa gtggttgata aacagttgcg tagacaggat
      601 tttctgacgt tcttcggcaa ccgtgagcca tgcctgattg gtatggaggc ctgtggaggt
      661 tctcagcact gggcacggga actgacaaaa cttggtcata aagtccggtt gttgcaagcc
      721 cgcttcgtta aggcattcgt catgggcaat aagaatgatg tgatggatgc ccgggctatc
      781 tggatggcgg ttcagcagcc gggtaaagaa atcgccgtaa aaacagaaga acagcagtcg
      841 gtactggttc tgcaccgtac ccgcatgcaa ctggtgaagt tccggaccgc acaaattaat
      901 gccctgcacg ggacgttact ggagtttggt gaaaccatcc acaaaggccg ggcagcgatg
      961 gagcgggagt tccccgaagc actggaacgg atgaaagaga gactgccacc gtatctcatt
     1021 atggttctgg aaaaccagta caaccgactg aatgagctgg actcactgat agaggatatt
     1081 gaaaaacagc ttaccagcgt ggcgaggcag aatgaaacct gtaagcggtt gctggatatt
     1141 cctggcgttg gaccacttat tgcgacggca gcggtggcca ccatggggga agcatcagcg
     1201 tttaaatcgg ggcgagagtt cgccgcatat gttggtctgg ttccaaaaca aactggctcc
     1261 ggagggaaag tacgtctgct ggggataagc aaacgtggtg acacttatct caggacatta
     1321 tttatccacg gtgcaagagc ggtggcatta gtagctaaag agcctggccc gtggataacc
     1381 gaactgaaaa aacgtcgtcc agccagtgtg gcaatcgtcg ccatggcaaa caagctggca
     1441 cgaacagtat gggcgataac cgcccatgac cgtaagtatg acaggaacca cgtcagtatc
     1501 agaccatatt aatcgctgat accattaaac aatgaactct taacaaaagg gtgaatgctg
     1561 aaaggttgct atggcggcca gagtgatgac aaagacaggt aagaccgtga ctcactaaac
     1621 ctgaacagta ttttgggctt gaagtccgcc gtgaaaataa ggggtgagtc ggcgaattac
     1681 ataggggctc gcagcgttac ggctacaata aagccggata taaagctgca acctacccgt
     1741 catgtcaaaa caatggatgc cttgcaaacg ggatgcgttc atataaaata ctgtaaataa
     1801 agccctgagg gttatgggat atgacacaac ctaggaagtc tgtggccatg gattccgggc
     1861 aatggcgtgc agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca
     1921 gatgagccat caggagcgta atggtgtacg tgctgcgtat atccataa
//
LOCUS       DAZFDP010000362         1967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFDP010000362 DAZFDP010000000
VERSION     DAZFDP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1051
                     /locus_tag="ACHJ1G_005439"
     CDS             <1..1051
                     /locus_tag="ACHJ1G_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HFO4767074.1"
                     /translation="IELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATD
                     GIKNVPNGLLSLSINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVT
                     IHVEQKTTWNIVAEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIV
                     YGLSSPREKLVNSIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHE
                     RGTKFKQCLKNHNNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLV
                     NSLKLIANKSEGYGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEW
                     KDYRPKYSGSTYKYS"
     gene            complement(1428..>1967)
                     /locus_tag="ACHJ1G_005440"
     CDS             complement(1428..>1967)
                     /locus_tag="ACHJ1G_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO4767075.1"
                     /translation="QLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQ
                     IYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQ
                     VRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLA
                     IIDRTFGMKENISMVNASK"
BASE COUNT          636 a          420 c          369 g          542 t
ORIGIN      
        1 tattgaatta actgattcac catttataca catcatatct gaaggcatcg aatgcctgac
       61 ggtttgccac tgccatatat ctggagtgcc agagagtgtc cgtcatcttg agatcagagg
      121 ttacgccaca gacggcataa aaaatgttcc aaacgggtta ttatctctca gcatcaatcg
      181 ctacacccca gaaaatcagg ccagaattga cagcctgata tcaccgtcac tacagacgct
      241 atctctgact ggatgtagca atattatact gccggagaaa ctcccggaga gtctgagatc
      301 tgtaaccatt catgtggagc agaaaactac atggaacatc gtagctgaag gggttcctga
      361 tggactggat cttgatttac aaaatgtact actctctccg aatgtaatta aagaaaaaaa
      421 catcactttt cagggcaacg cactggatgc tgccttgcat tttcgtccgg gagacattgt
      481 ctatgggcta tcctctccca gagaaaaact tgtcaacagc atcaaactgg ttaacgactt
      541 ttccaaaaaa gatatcgtaa ttcagaatac attaacaaac gcagtatggg acgccagaac
      601 cccccgtaaa tataagcaag actcgcttat caaaagagca ttaaatgaac atgaaagagg
      661 aacaaaattt aaacagtgct taaaaaatca caataattat aatgtcatca tggccgacct
      721 ttccatatac aatcgagaca agttatgggc aaaaacaagc aaggcaggcc tagagtttca
      781 aacattaatt cgcaacagaa cggttatgtt ttgtgcggat gggcttgtca attcactcaa
      841 actcatagct aacaagtcag agggctatgg tcagagtatt accgccagcg aattacgatg
      901 gatttatcgt aataaagaca acagccaaat aatgaaaaac ataaaatttt atctgcatgg
      961 caaagagata ccagcagaaa gaatattaga tacaccagag tggaaagact accgtccaaa
     1021 atactccggt tccacatata aatattctta atgatagcaa aaaatatatt ttcgatataa
     1081 tcaatgttat gattaaagag tatttcatca gggcaggtaa aaacagagta aatcagcaaa
     1141 agaagctgat cttcagcgat actgacacta actgacggtt taagcggtcg tatgaagcag
     1201 cagctttccg acggactgcc atgcggatcg tttacctttt gggctattcc gcccgtcatc
     1261 aagcggctca cgagtactga gtttatcagg atgatattgc tgacaatggt aattcgttga
     1321 ccgatgtgta cttcactata tatcggtcaa caccgtactg ccgtgcaaca tctcaaagat
     1381 gtagcatcgt ttttaccaga ggaggcagga catagattgt aaaaaaacta tttcgatgca
     1441 tttaccattg agatattttc cttcatacca aaagttctat caattatcgc aagtggttca
     1501 acaggtgcgg tcttatctat aacccaacca ctctctgtaa cacttcctcg tgtgatatat
     1561 tttgccttta ctttaacaac tattagataa tgctcacgac taaaccgatc agcaacgcta
     1621 agatccgatg tgtattcagg caatattctt ccaatacgta cctgctgttg tgccatatat
     1681 tcatctggtg cttttacatt tggatttgaa tcaggaagcc ctccagcagt tttatattca
     1741 acgatatttt cagcttgaat accggttgtg ccgcgacaca atatgatgtc tgaatcggac
     1801 agttctatgt gcccctcctt aattttcgaa taaatttgtt taatactatg ttctggagtt
     1861 ggtactattt cagaagataa agcaggcaac gtgtcttcaa gcaccggaga atcatcgtat
     1921 atatccaaca aatcctcatc atacccaata tctccttctc ttaactg
//
LOCUS       DAZFDP010000363         1952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFDP010000363 DAZFDP010000000
VERSION     DAZFDP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1123)
                     /locus_tag="ACHJ1G_005441"
     CDS             complement(<1..1123)
                     /locus_tag="ACHJ1G_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HFO4767076.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSR"
     gene            complement(1136..1714)
                     /gene="espA"
                     /locus_tag="ACHJ1G_005442"
     CDS             complement(1136..1714)
                     /gene="espA"
                     /locus_tag="ACHJ1G_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO4767077.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(1772..>1952)
                     /locus_tag="ACHJ1G_005443"
     CDS             complement(1772..>1952)
                     /locus_tag="ACHJ1G_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO4767078.1"
                     /translation="WLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRDNA
                     LLMIGKVIDYKEDVI"
BASE COUNT          527 a          464 c          385 g          576 t
ORIGIN      
        1 tacggctatt tactcgtgct gagtcggata ttaactgaga agcattttgt aaatagcttg
       61 cctggtttcg caactcggct gcagctttat ccagctcaac cttcgcactc tcacccaggt
      121 tttcactctg tcgagttaag ttttgtaccg cgtaagcaga taactgagcc aatatgctgt
      181 attgcgcaga atcattattc agcacagttg tcccaataga acgtgcagag gtcgtaaatc
      241 catttgccgt tgccgcaact tttgaaacaa cgttctcaac aaatacatcg gccagtttag
      301 taactgtttt cacgatgttt gaaccaactt tgttagcgac atcgccaact ttagacataa
      361 gtgaagacac cccgccaatg ccggctgtca gaatacttgc agcaatagat actccgccaa
      421 agacttgcgc tgctgtcttt aacgcctgcg gggccttatc gcccattaca tcaactgcag
      481 tttgcagtgc cattgctgtt gcgctaacgg caacaacagc ccagagtgcc gggttaaaaa
      541 tagctgcaat agcagttacc gcgacgccca accaaccaaa gacctgacca agaattttac
      601 ttttttgtgc tttctcttcg gctttctgtt gttcttcgag ctgcttttta aactccagcg
      661 ttttattatc cagcgcctta ttttgcccat ccgtgtagat ctcgttggag tttttcatac
      721 tcgcaacttt ctgcgctgaa gcatccaggg ataacagggt gaccatcatc atcatggcct
      781 gagggtcaac gttattcacc tgagaaagat aaggatagcc agagcttgca ttctgagtgc
      841 tgcgctgtgg ttcggttccc tctgttcgac tcctaacttc accactaata ccaccaaaca
      901 gtttactcag gacatcctca gcaacagagg gcgtcactaa tgagtggcct gccggtggcg
      961 ttggtaaagc ggtactttct gtccaacttg ctgatggggc cgtggattta accagttgta
     1021 aatccagcgc ggaagtcact tcaggctgat taatacctga agtagccgta gcagcgctgg
     1081 ccccagacct catagactgg atatcgctat ttacattaag catagttatc tccggttatt
     1141 taccaaggga tattgctgaa atagttctgt attgcagtga ctgaatatca gaacgtgcac
     1201 tcgttaatag gtttaacgta tttgacattt gctgtatttc aagctggcta ttattcacta
     1261 ccgtggtcaa gttattcgat ttggccgaaa tagcggcctt caccgtttgc aaatcaccag
     1321 cgcctaattc agcatttaga tcgctaatac cacttactgt aatgtcattg cgaggatcat
     1381 ttatatagtc aatcacttct tgaggaagtt tggctttctt attcttgtca gaactactct
     1441 gaacatcagc aattttagca tccacaagat tagccatttt ttgggctgtg gttgactcct
     1501 tagatgcctc attcatatca gcaaactttg caatcgacag atcgctttgt gcctgataca
     1561 tataggcaaa catgagaagc gcagcctgaa aaacaccgac tttattaaat agctgaacta
     1621 cttcgtcttt cgacatactg cctaagtcat agactgtcga tgtcgaagta ctcgcgttcg
     1681 cactagcaac tgatgttgca gttgatgtat ccatgtatat acctcttaat aatctttctt
     1741 agcaagacca aaaacgatta aaaaaccaat atcaaataac atcctcctta taatcaatta
     1801 ctttaccaat cattaataat gcattatctc tctgttccct atcaataaaa caagcgtcag
     1861 gtaatgatgc aaaaaagtct ttcaataggt ggaaataata aacatgttta tcttcgacaa
     1921 tcgatacccg agaagggctt tccaggagcc ag
//
LOCUS       DAZFDP010000364         1941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFDP010000364 DAZFDP010000000
VERSION     DAZFDP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..202
                     /locus_tag="ACHJ1G_005444"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHJ1G_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHJ1G_005445"
     CDS             331..576
                     /locus_tag="ACHJ1G_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767079.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHJ1G_005446"
     CDS             548..958
                     /locus_tag="ACHJ1G_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4767080.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHJ1G_005447"
     CDS             955..1131
                     /locus_tag="ACHJ1G_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4767081.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1134..1535
                     /locus_tag="ACHJ1G_005448"
     CDS             1134..1535
                     /locus_tag="ACHJ1G_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767082.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            1495..1704
                     /locus_tag="ACHJ1G_005449"
     CDS             1495..1704
                     /locus_tag="ACHJ1G_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4767083.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            1697..>1941
                     /locus_tag="ACHJ1G_005450"
     CDS             1697..>1941
                     /locus_tag="ACHJ1G_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4767084.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQ"
BASE COUNT          612 a          386 c          490 g          453 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
     1141 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggc gagaaagagt
     1201 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1261 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1321 tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca tacatgaagg
     1381 cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg gaaaagcttg
     1441 aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac attgatgctt
     1501 acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct tcaataccag
     1561 aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     1621 gaagttcacg tgtgtgagta ctgctgcgca gaactgatga gcgatccgaa tagctcaatg
     1681 tacgaggaag aagacgatga atgagttaat aaatggcaat gccatcaaaa tgacaagcat
     1741 tgaaatagct gagttggtgg gtaagcgtca tgacaatgtg aaacgtacca tcgaaacgct
     1801 ggctaaaaat ggtgttatcc ggcttcctca aattgaggtt tccgaaagaa tcaataactt
     1861 agggttcaat gttcagtacg agcattacgt cttcgagggc gaacaaggta agcgagacag
     1921 tattgtcgtt gttgcccagt t
//
LOCUS       DAZFDP010000365         1930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFDP010000365 DAZFDP010000000
VERSION     DAZFDP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..770)
                     /gene="nleH1-2"
                     /locus_tag="ACHJ1G_005451"
     CDS             complement(<1..770)
                     /gene="nleH1-2"
                     /locus_tag="ACHJ1G_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO4767085.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVL"
     gene            complement(877..948)
                     /locus_tag="ACHJ1G_005452"
                     /pseudo
     CDS             complement(877..948)
                     /locus_tag="ACHJ1G_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            942..1930
                     /locus_tag="ACHJ1G_005453"
                     /pseudo
     CDS             942..1930
                     /locus_tag="ACHJ1G_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          579 a          461 c          397 g          493 t
ORIGIN      
        1 tataaaacat ttgtttctgt tgtatcaaca aaaagaattc cttttttctc cagtctgtca
       61 aacatatcgt aaatagcctg ttcagcttgt gctggtaatg atggaatatc caaaagagat
      121 tccccattta ttttattcat tctaatacca ataacatttc cattatcatt atatattttc
      181 tctgcactcc cggaaccata atactgattg aaacaacgaa cttcgcttgt cacctcttca
      241 tggctttgag atatagtaaa catcttcaac acttttgttg tatcttccat atcttcatac
      301 acgacagcat taccaccttt accaataaca ttacctggca cgggtgattt attgttgcta
      361 accctcggca actctgattg agtataatcc gctggaggta actccggctg cgcataatcc
      421 actggaggta aatcaggtct gttcgaatga acagagcctc tttctatagt cgtgctcact
      481 ggcgatgtat tcagcatagc ctcaattttt ctgctaatct ctcccttagg ccatcccaac
      541 ctgtgcaaca gattagtaaa acaaccacta tgactttctc ttgtaacgca aaacttatta
      601 tcagtgacca caacacgata tgttctgttg ccaaccgtta cttgcgtccc gctatcagag
      661 tgaacagcag catcccttac agaggataaa acacgattat caggcgaagt caggtttctg
      721 gttaaagaat tccatgaaca tcccaaattt atagaagagg gcgataacat acatttcaac
      781 cttcaaaata aacctatcta attattccta acagacatcc cccatacatg acaacaaaaa
      841 ccggagccgg actccggttt ttgtgaagct atcgggttac ttcatttcgc caatattttc
      901 ccacttcccg tcagcacgca ggatttgcag cggtcttact acacactgac ccgaggacga
      961 aaaaagagtt cgggctggga agagacagaa gagtagcaat ttcagaagca ttcacaccaa
     1021 cattgagctt ttcagcgaaa gtgggcacga atcattgctg gacagggtta aaggcactga
     1081 ctcaatcact cttcatacgt ggcttgatcg atatgaaaca atcctcagct agagggggag
     1141 caagccgaaa acattactcg actacgccag caaaatcagg gcaattcgaa gaaaattgcc
     1201 ggacaaaccg ctcactgaca tatcaacgaa agaggtggca gcaatgctaa acacctacgt
     1261 cgcagaaggt aaagcggttt ccgcaagagt aatcaggtca acccttgttg acgtttttcg
     1321 aggggcaata gccgaggggc atgtggcaac gaatccagta acaacaaccc gtgcagcaaa
     1381 gtcagaagta aggcgctcaa ggctgacagc taatgagtat gtcgctattt accatgctgc
     1441 tgagcacctc cccatctggc tgaggctgtc aatggattta gctgtcgtta cagggcagag
     1501 agtgggcgat ttgtgcagaa tgaagtggtc agacataaac gatggtcatc ttcacattgg
     1561 acagagtaaa acaggagcca aaattgccat tccgctggct ctaaccattg acgcactcga
     1621 catctcactg gttgatacac tacagaaatg cagggaggcc agcagcagtg aaacaataat
     1681 cgcatcaacc tatcacgaac cactttctcc agccacagta tcacggtatt taacaaaggc
     1741 gcgaaatgca tccgggatct cgtttgatgg agacccaccg acatttcatg aactacgtag
     1801 tctgtccgcg aggctatatc ggaaccagat tggctacaag tttgcacaac gtcttcttgg
     1861 acataaatct gattcaatgg cggcgcatta tagggacagt cgcgggcggg agtgggacaa
     1921 aattgaaatc
//
LOCUS       DAZFDP010000366         1908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFDP010000366 DAZFDP010000000
VERSION     DAZFDP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1816
                     /locus_tag="ACHJ1G_005454"
     CDS             <1..1816
                     /locus_tag="ACHJ1G_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767086.1"
                     /translation="NKVVFKVDFASLQKARDAGKKFQKEMENLTNPTLKGKKAKRAID
                     EAHDLVKARKAAQAKTDAKASDKQAQTDKKNADKVAKAEAKAKAKAAIAADKQIRAKA
                     TIAEKAEIKRQQAFRQLEGIKKGRMGDGMDSAQYYKTLQYIKAQTAAYEKGAISQAKM
                     NHLIRDRITLERRAAAVKARQVAADGGKYPHMAKQLKGGHIGGGVGAMVGGAALTAAT
                     LGGAAIFNTVTDQASKITDTNRKAKAVNVNTNALLALNQYGLEHGVDSSVDQRQDNLK
                     DVQERLGESVGNTKWDAKKGRWAGGNGAVDNILNKYGWSPEQIKGFQTDPLGFLGAVV
                     KEGERRGLSDQEQGHILEDLGNDLQYFRGAFSRNGEELNKTIVKMQKANATYTQADLD
                     AAERLQAVQANMNMAWEGSKKAIMTGFGEEFDAQAGEEWAQMLKDLNPLFKELGSSTA
                     EMMSAFAGAVSWLTAAYQNINPENKHENLDSSGMYYKDSFVGGMVDWGRSALGLSPIP
                     GTEDKPTTGGTAIQNYSSPSDNVQSLKENVSRDLNTTPSMTYAPSIVVNVPESKVDVN
                     IAPDGSQLGSYLDYKVGSIQQDFERSFTLAALSGQAPS"
BASE COUNT          595 a          398 c          490 g          425 t
ORIGIN      
        1 aaacaaggtt gtatttaaag ttgattttgc atcattacag aaagcaaggg acgcagggaa
       61 gaaatttcag aaagaaatgg aaaacctaac caatccaaca ttgaaaggga agaaggctaa
      121 acgagctata gacgaggctc atgatctggt aaaggctcgt aaagctgcac aggctaaaac
      181 tgatgccaaa gcgtcagaca agcaagctca aaccgataag aagaacgctg ataaggtggc
      241 aaaggcagaa gctaaagcga aggctaaagc cgctatagct gccgataaac agatccgcgc
      301 taaagcgaca atagcagaga aagcagagat taagcgccag caagctttca gacagcttga
      361 agggattaag aaagggcgta tgggcgacgg tatggactcc gcccaatact acaaaacgtt
      421 gcaatacatc aaagcacaaa cggcagcgta tgagaaaggc gctattagtc aggctaagat
      481 gaatcacctg atccgtgatc gtatcacgtt agaacgccgt gcggctgctg taaaagctcg
      541 ccaggtggcg gctgatggcg gtaaatatcc acatatggct aaacagctta aaggcggtca
      601 tataggcggt ggtgtaggtg caatggtggg cggtgccgct ctcactgctg ccactctcgg
      661 cggtgctgcg atattcaata ccgtaaccga tcaggcaagc aagatcacag acactaaccg
      721 caaggcaaag gctgtcaacg taaacactaa cgccctgtta gcacttaatc agtacggctt
      781 agaacacggt gtcgatagtt ccgtagacca acgtcaagac aaccttaaag acgttcaaga
      841 gcgtttaggt gaatccgtag ggaataccaa atgggacgct aagaaaggtc gctgggctgg
      901 cggtaatggt gccgtcgata acatccttaa taaatatggt tggagtcctg aacagattaa
      961 aggtttccag actgatccat taggtttcct gggcgctgta gtgaaagaag gagaacgccg
     1021 tggactctct gatcaggagc agggtcacat ccttgaagac cttggaaatg atttacaata
     1081 tttccgtggt gctttcagtc gtaacggtga agagctaaat aaaaccatcg ttaagatgca
     1141 aaaagccaat gccacctata cgcaagcgga tcttgacgct gctgaacgtc tgcaagctgt
     1201 acaggctaat atgaatatgg cctgggaagg tagtaagaaa gctatcatga cgggattcgg
     1261 tgaagagttc gacgctcagg ctggtgaaga gtgggcgcaa atgttgaaag atctcaatcc
     1321 tctgtttaaa gaacttggaa gctcaacggc tgaaatgatg agcgcctttg ctggtgctgt
     1381 atcgtggctt actgctgcat accagaatat aaatccagag aacaaacatg aaaaccttga
     1441 ttcgtccggt atgtattata aggattcgtt tgtaggcggc atggttgatt ggggtcgtag
     1501 tgctttaggc ttatcgccta tccctggcac ggaagacaag ccaacgactg gcggaactgc
     1561 aattcagaac tattcaagcc cgtccgataa cgtgcaatcc ctgaaagaga acgttagccg
     1621 tgatctgaac actaccccat ctatgacgta tgcgccaagc attgtcgtaa acgtgccaga
     1681 aagtaaggtt gatgtgaata tcgctcctga tggtagtcag cttggatcat acctcgatta
     1741 caaagtggga agtatccaac aagattttga acgtagtttc acgttggcgg ctctgtctgg
     1801 gcaagcacca agttaatgat aaagagaatg tttaatcacc atttaaaaca ctcttataag
     1861 gaattaagca tataagaggc tcccattaca aggaagcctc tttattgc
//
LOCUS       DAZFDP010000367         1896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFDP010000367 DAZFDP010000000
VERSION     DAZFDP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..485)
                     /locus_tag="ACHJ1G_005455"
     CDS             complement(<1..485)
                     /locus_tag="ACHJ1G_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO4767087.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TV"
     gene            complement(496..666)
                     /locus_tag="ACHJ1G_005456"
     CDS             complement(496..666)
                     /locus_tag="ACHJ1G_005456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767088.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHJ1G_005457"
     CDS             complement(742..894)
                     /gene="kil"
                     /locus_tag="ACHJ1G_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO4767089.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(879..1013)
                     /locus_tag="ACHJ1G_005458"
     CDS             complement(879..1013)
                     /locus_tag="ACHJ1G_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767090.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(1089..1400)
                     /locus_tag="ACHJ1G_005459"
     CDS             complement(1089..1400)
                     /locus_tag="ACHJ1G_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO4767091.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            complement(1544..>1896)
                     /locus_tag="ACHJ1G_005460"
     CDS             complement(1544..>1896)
                     /locus_tag="ACHJ1G_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767092.1"
                     /translation="YIAFHQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKK
                     VAKNAERSFAFPTKKLAIESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEY
                     NFGHNQETENWVFY"
BASE COUNT          465 a          435 c          372 g          624 t
ORIGIN      
        1 acggtgtttt tctgcaatgc tgcgtcagtg aatgctttta gaacctgttc aggtgtaggt
       61 gatggttttg actgctttgc tgctgcgttc tgctgatgtt tatgctcgtc ggtatctgca
      121 tctttcgcat catcaatgcc gaacaaacca ttgaggcaat acttgcgtgc ataagagctt
      181 gtagctcccg taacttgtgc agaatccatt cctttcttgc tttcttcctc tcgtgcaaga
      241 gcggttgccg tatgactgtt ttcgccatcg gtaatagttg ccgtggcttt cacataatac
      301 cgatcaccaa tcaacacaac ttcatcgctg attgataaaa acaggccatt cagtaacggc
      361 ttaacgcctt caagaatgtc ttcgcagctt ctgtatttat atttaccgaa tgagttgtac
      421 tgattctttg gcgcgttcaa gttctcctga atagctgcca gtcttgcgta aaagtctttg
      481 ctcatatgtt tgttctcaga atgggcatgg cccaaggaaa taacgctgat ttaatacttc
      541 agtctttgcc gcatttaaaa atacgcgaac accttcacga tctcccttct ggcgatacat
      601 taacgcctgc tgcgtgtaca tgcgtctctg taacttgctc tccttcactg tggttgcaag
      661 tgacatgaat atctccttcg ttaccgatta attctttcat ctgacgaatg aattcttcgt
      721 ctgaccagtt atctgtgaaa ctcatttcct gcgataccac ggaaggttga tagctgattt
      781 catcgcttta tttgcttcaa gccacatttt tgaatcacca ataaatctgg ctattactgc
      841 tttgttttgt gccgcacgaa gcatctggtg attaatggct atttcattgc gcataacgcc
      901 tccagttgtt tctttgctgc tctaattaat tgtttaactc ggcgtgataa ttcagattcg
      961 tgcgggtaga aagcggacat gacgccgcta cccgcgagct gaaagtgcat catgggtaac
     1021 tccttatatt tgattgcata acgaaaacgc ctcgagtgaa gcgttattgg tatgcggtaa
     1081 agccgcgctt aggcggctga tgtttcttct ttcaggcttt cgagatattt acgtgggtcg
     1141 tcgtaacatt ggcattcgct gtaccaatcc acccagcgat cagtaagccc cacctctgat
     1201 aaatcttcat cggtaaggct ctcatcccac atctcaaggc cgttagcatt gcagtaatca
     1261 ggcttgatgt tgttgtcata ctgaaaggcg tcataatcag ccagtgcgcc catcaggcga
     1321 acaccctctt caacacttgc cacttctgca atgaacggtt tcataggtac ttgcgggata
     1381 tgccagacac gtaatttcat atttcctccg tcaaaaaaat cgccctcaca ttggagggca
     1441 aagaagattt ccaataatca gaacaagtcg gctcctgttt agttacgagc gacattgctc
     1501 cgtgtattca ctcgttggaa tgaatacaca gtgcttattc gtactaataa aatacccaat
     1561 tttctgtttc ttggttgtgt ccaaagttat attcaatatc tggtgttgat gtatcaatat
     1621 ttttcatccc atcaacaaga gttgatacaa cagccaaatc ttgttttatt ctcattaaat
     1681 ggtatttctt ccggcgcaat aaactttcaa tagcaagttt cttcgttggg aatgcaaaag
     1741 atctttctgc attttttgct actttcttaa ttgcatatct atttctcttt tgtttccatt
     1801 cctgtaacca ctgatttggt gctggtttaa aattaacaat ccaatgcgca ggaaccaacc
     1861 atgcataatg ctctgtctga tgaaaagcta tatatt
//
LOCUS       DAZFDP010000368         1895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFDP010000368 DAZFDP010000000
VERSION     DAZFDP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_005461"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHJ1G_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4767093.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005462"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHJ1G_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4767094.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..>1895
                     /locus_tag="ACHJ1G_005463"
     CDS             1077..>1895
                     /locus_tag="ACHJ1G_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO4767095.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTR"
BASE COUNT          448 a          530 c          512 g          405 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccgg
//
LOCUS       DAZFDP010000369         1894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFDP010000369 DAZFDP010000000
VERSION     DAZFDP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHJ1G_005464"
     CDS             <1..480
                     /locus_tag="ACHJ1G_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4767096.1"
                     /translation="LNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYVGA"
     gene            480..806
                     /locus_tag="ACHJ1G_005465"
     CDS             480..806
                     /locus_tag="ACHJ1G_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO4767097.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVAAMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTTVKDDDSEAAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            803..1192
                     /locus_tag="ACHJ1G_005466"
     CDS             803..1192
                     /locus_tag="ACHJ1G_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4767098.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            1212..>1894
                     /locus_tag="ACHJ1G_005467"
     CDS             1212..>1894
                     /locus_tag="ACHJ1G_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4767099.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTA"
BASE COUNT          468 a          459 c          559 g          408 t
ORIGIN      
        1 ttaaatgacg ttcagaaatt cattgaagcc catccggggt gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt ctgcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggagccgga accacaacca gttcgtgaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc ccgggtgatt ttgtgcctga cccgccaggc ggaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcagtgttt acggaaaagc
      421 agcaagcgcg ctgtatcggg tgatgagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct tatcagaaag
      541 aacgtggttt cccgccaacc aatcaggagg tggcagccat gctgggatac cgttcggtga
      601 atgcagcggt ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg
      661 gcgtggcccg gggtatcacg cttcatacca cagtgaagga cgacgacagc gaggcagccg
      721 ggattatccg cgcactgctt gccggtgagg aaaacgccag actgcgtgca gcccactggt
      781 tacatgagag gggcctgaaa gtatgaagct gatcctgcct ttcccgccca gcgtgaacac
      841 gtactggcga caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg
      901 gcgcaaattc cagagcgcgg catgcgcagc aatagttgag cagttacgtc gtctgccgaa
      961 accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg
     1021 cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga
     1081 agacgacaga caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa
     1141 ggtcgagatc actatcagta agtatgagaa accggcgggt gcagccgcct gattaagagg
     1201 agaaacgaag tatgaataat ctgatggtta ttgatggtat tgaagttcgt cgtgatgctt
     1261 atgggcgtta cagcctgaac gatctgcaca gggctgctgg ttctctggat aagcataagc
     1321 ctgcattctg gctccgcaat gagcaaactg agcgtttaat aagcgagttg cagatttgca
     1381 actcggtcaa tatagagcca gttaacgtta ttcgtggcgg aaataaccag gggacgtatg
     1441 tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg
     1501 tgatccgtac tttcgacatg gtaaccagcg caccggaaaa attatccgga caggctgctg
     1561 acaagatgca ggctggtgtg atcctgctgg actttatgcg ccgggaatta aacctgtcta
     1621 actcatcagt gcttggagcc tgtcagaagc ttcaggaggc tgttggctta ccgaatctgg
     1681 caccgcgcta tgccattgat gctcctgctg acgcgcctga tggctcaagc cgccccacgc
     1741 tgtcactgag tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc
     1801 accagatggt gaagctgggg atcgtcgagc agcgcgaacg atacagccgt accgcgatta
     1861 acaacatcaa aaaattctgg tcgctgacag cgaa
//
LOCUS       DAZFDP010000370         1884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFDP010000370 DAZFDP010000000
VERSION     DAZFDP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..387
                     /gene="bssS"
                     /locus_tag="ACHJ1G_005468"
     CDS             133..387
                     /gene="bssS"
                     /locus_tag="ACHJ1G_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4767100.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            502..1620
                     /gene="solA"
                     /locus_tag="ACHJ1G_005469"
     CDS             502..1620
                     /gene="solA"
                     /locus_tag="ACHJ1G_005469"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4767101.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1668..1781
                     /locus_tag="ACHJ1G_005470"
     CDS             1668..1781
                     /locus_tag="ACHJ1G_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4767102.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          484 a          455 c          447 g          498 t
ORIGIN      
        1 cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt attggtatac
       61 tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag taacacgaat
      121 tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct cgtagggtgg
      181 gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta ccagacccca
      241 aataagtccg agctggaagg gactgaagtt ggtcagacgc tctggttaac cactgatgtt
      301 gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc cggtgatttc
      361 ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa aggcacctca
      421 ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact ctgcttaaac
      481 catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag cggttccgta
      541 ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc
      601 catatgccac cgcatcaaca cggcagccac catggcgata cgcgattaat tcgccatgct
      661 tatggtgaag gcgaaaagta tgtcccgctg gtcctccgcg cgcaaacgct gtgggatgaa
      721 ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat taacctcggc
      781 ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac
      841 gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg cgtcccggac
      901 aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact ggcgattaaa
      961 acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg cccggtcacc
     1021 gtaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga gtatcaggcg
     1081 aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga gctgcctgtc
     1141 cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag cgtgaagaat
     1201 aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg ttttccggca
     1261 gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca ttcagcggat
     1321 gaacgtgttc cgtttgcgga agtggtcagc gatggttcgg aagccttccc gttcttgcgc
     1381 aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta tgataattcg
     1441 cctgacgaag attttattat cgatacccta cccggccacg ataatacact gctcattacc
     1501 ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc tgccgatttt
     1561 gcgcaagaca aaaaaagcga ttttgatttg acgccattca ggctttcccg cttccaataa
     1621 tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg cgtcggctgt
     1681 tgcactatct catcaataat attcgcgagc atctgatgct ttatcttttt ctctggggat
     1741 tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca ctgacataaa
     1801 ctgacaaata tttaatctcc ctttcatttg gcgtgaaaaa ttgaatgaga ttattcctgc
     1861 ttctacagca ctcaccagac atct
//
LOCUS       DAZFDP010000371         1861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFDP010000371 DAZFDP010000000
VERSION     DAZFDP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1733
                     /locus_tag="ACHJ1G_005471"
     CDS             3..1733
                     /locus_tag="ACHJ1G_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO4767103.1"
                     /translation="MLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEA
                     EQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPG
                     TARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNY
                     FPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNL
                     VFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKA
                     EINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSI
                     ESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMR
                     QTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLL
                     FELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTI
                     NAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIE
                     LADGVTDISQ"
BASE COUNT          538 a          410 c          470 g          443 t
ORIGIN      
        1 tgttgcttat gagtagtttg attttgtcag gatgcgggcc ggaatctgag aataaggaaa
       61 gtcagcaaca acagcccagt acccccacag accagcaagt gcttgccgcg caacaagctg
      121 caataaaaga ggccgagcaa agatccgtcg ccgataaagc cactgcagac gcgaaagcaa
      181 aagccttagc ccagcaagaa gcgcaacaat attcagacaa acaggctttg caggggcgct
      241 tacaggcggc tccaaaatat caacatgcag ccagagaaaa agcagcgtcc caaatcgcaa
      301 atccaggaac cgctcgctac aagcagttcg atgataatcc ggttaagcag gtagcgcaaa
      361 acccgttggc gacgtttagc cttgacgtcg acaccggcag ttatgcgaat gtacgccgtt
      421 ttctcaatca tgggctgtta cctccgccag acgctgtgcg ggtggaggag atagtcaatt
      481 atttcccgta tgactgggat atcaaagaca aacaatctat tccggccact aagccaatac
      541 ctttcgctat gcgctacgaa ctggcacctg cgccgtggaa tgaacagcta acattgctga
      601 aaattgatat cctggcgaaa gatcataaaa gtgaagagtt accagcttct aatctggtct
      661 ttcttatcga cacttctggt tcaatgattt ctgatgaacg tttgccactt atccagtctt
      721 cgttgaaatt attggtcaaa gaacttcgtg agcaggataa cattgccatc gtgacctacg
      781 ctggcgactc ccgtattgcg ttgccttcta tctccggtag tcacaaggcg gaaattaatg
      841 ccgcaattga ttcgctggat gccgaaggca gtaccaatgg cggtgccggg ctggaactgg
      901 cttatcagca ggcggcgaag gggtttatta agggcggcat caatcgcatt ttattagcca
      961 ctgacggtga ctttaacgtt ggcattgacg atccaaaatc gattgaatca atggtcaaaa
     1021 aacagcggga gtctggtgtt actctgtcga cgtttggcgt ggggaatagc aattacaacg
     1081 aggcaatgat ggtgcgaatt gccgatgttg gtaacggcaa ctacagctac attgataccc
     1141 tctctgaagc gcagaaagta ttgaatagtg aaatgcggca gacgttgatt accgtagcaa
     1201 aagatgtcaa agcgcaaatt gagtttaacc ccgcgtgggt aacggaatac cgtcagattg
     1261 gttatgaaaa gcgccaactt cgggcggaag attttaataa cgacaacgtt gatgcggggg
     1321 atattggcgc aggcaaacat ataacgttgt tattcgaatt aacgctgaac gggcaaaaag
     1381 catcaattga taagttacgc tatgccccgg ataacaaatc agtgaaatcg gacaaaacga
     1441 aagaactggc ctggttaaag attcgctgga aatacccgca gggaaaagaa agtcagttag
     1501 ttgaattccc gctggggcca acaataaacg ccccctctga agatatgcgt ttttgcgcag
     1561 cagtagctgc atatgggcaa aagttacgcg gttctgaata cctgaacaat acctcctggc
     1621 agcagatcaa acagtgggct cagcaggcaa aaggggaaga tccacagggt tacagggcgg
     1681 aatttattcg cctgattgaa ctggcggatg gtgtgactga catcagtcag tgatgactgt
     1741 ttagcaaact atgttcgacc agtcagcata tttgctgact ggtcgaattg attaacaatg
     1801 atgttaactc actcttttgc cggatgctct atttttgggt tgtgagtgtt ttatttctaa
     1861 t
//
LOCUS       DAZFDP010000372         1858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFDP010000372 DAZFDP010000000
VERSION     DAZFDP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHJ1G_005472"
     CDS             222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHJ1G_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4767104.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHJ1G_005473"
     CDS             1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHJ1G_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4767105.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          498 a          328 c          456 g          576 t
ORIGIN      
        1 gtaacatcct gtgtatcaat aaatgttgtt atctacgtac gtcaagtagt cgcatgagat
       61 ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt tcattacgtt
      121 attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg aggatgtcaa
      181 gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta
      241 gagtgctaac ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct
      301 tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag
      361 gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggct
      421 caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta
      481 ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga
      541 caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag
      601 cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc
      661 gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc
      721 atagtggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga
      781 cagctgaagc tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc
      841 tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa
      901 ggttgagtag cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt
      961 cttttggaag cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc
     1021 atgcatcgcg agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg
     1081 gaagagtccg tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa
     1141 ttctgatgcg cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag
     1201 ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa
     1261 aggtggagta tacaaaatat aatgatgacg atacctttac agttaaagtg ggtgataaag
     1321 aattatttac caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga
     1381 tgactgtaac cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt
     1441 ttcgttgact cagaatagct cagtgaaaat agcaggcgga gattcataaa tgttaaatac
     1501 atctcaattc agtcagttgt tgccggtctg ataatagatg tgttagaaaa tttctgcatg
     1561 gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa
     1621 gcgagaatac gcgggtttgg tggcaccagg ccgaactcac cgggaggcac ccggcatcat
     1681 gctgtataca gagattaggc atatatccag gctcctcatc gcaggagcct ttttacatgc
     1741 aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata cttgcattgt catttttatc
     1801 gtgcggattt taaccaggat tcataaggct gcgcaactgc gcggcctttt tcgtattt
//
LOCUS       DAZFDP010000373         1853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFDP010000373 DAZFDP010000000
VERSION     DAZFDP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..463)
                     /locus_tag="ACHJ1G_005474"
     CDS             complement(<1..463)
                     /locus_tag="ACHJ1G_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4767106.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIA"
     gene            732..848
                     /locus_tag="ACHJ1G_005475"
                     /pseudo
     CDS             732..848
                     /locus_tag="ACHJ1G_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(953..1029)
                     /locus_tag="ACHJ1G_005476"
     tRNA            complement(953..1029)
                     /locus_tag="ACHJ1G_005476"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(993..995),aa:Arg,seq:tct)
     gene            complement(1043..1119)
                     /locus_tag="ACHJ1G_005477"
     tRNA            complement(1043..1119)
                     /locus_tag="ACHJ1G_005477"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Arg,seq:tcg)
     gene            complement(1127..1202)
                     /locus_tag="ACHJ1G_005478"
     tRNA            complement(1127..1202)
                     /locus_tag="ACHJ1G_005478"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1167..1169),aa:Ile,seq:cat)
     gene            complement(1231..>1853)
                     /locus_tag="ACHJ1G_005479"
     CDS             complement(1231..>1853)
                     /locus_tag="ACHJ1G_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767107.1"
                     /translation="LATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTD
                     VPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGCLSSI
                     SAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNM
                     LCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          483 a          481 c          442 g          447 t
ORIGIN      
        1 tggcaatatg taagccctgg ccgacacagc cgtactgccc tttgctcagg tctgccttcg
       61 gatgattcag cgtactcata tcctgcacat catgcaggca gtggtcagcc ggaatgatgt
      121 cgttatacgt acaactctct ccacccggag ttaccgtgct gcggcgcgcc agctgtttaa
      181 tgcgcggatc cggagcatcg taagaatccg gtaacggaag cccttcaccg taggccatgc
      241 cgttggactg tccggcaagc acaaccacgt agaaccagtc cggctcagat gaagggccga
      301 cctgtggatc tccttcaata gccaccgcct gcatcagtgt gtacggcgta atggcaaccg
      361 gtccgccgta tggctgccag ccctctttca gtttgtgtgt cagcttttcc gcaaggtctg
      421 acggcgacgc cgccctgaca acatcatagt gtttaaatgc catgaatcct cccggccggg
      481 ataatattgt gagtaaaatg aggagcgggc tgaagtccgg aagttacagg acaatggcag
      541 aagagagaca acagcccgca atacgaaaaa ggccgcgcta ttgcgcagag tgattactgt
      601 cggatattat tcgccagctg aaatattact tcacgttttg ttgtttattc cttgccgccc
      661 gcgtctccct gcgcgggctt tttttgtcca taagaaagcc cctccggaga ggggctggag
      721 agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca gtaccagcac
      781 ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac agggggattc
      841 accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa ccgcctgaat
      901 tgtgagacat ttaaaaaaaa ggcccgcaaa agcgagccag ggaaaataag tgtggcgcgt
      961 tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc
     1021 taacaacgca ggatacagat aatggaccgc cttcggggac ccgaactccg cgcaaccagc
     1081 ttcgaaagct ggcgctcttt cctgatgagc taatggcggt atgtgatggt ggcccttgct
     1141 ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg
     1201 ccgggtcaaa aaataataat cagatgaaaa tcaataatca agcccttgcc tggatacata
     1261 tctgtctggc gggaagccat aatagcggtg aaatacagaa ataaagtagg accagcttga
     1321 ataaccgcat ttttctgcta cagcctgtcc atatccatgc cgggaacata acatattcac
     1381 agcaacacgc atccgctctt ccagcaacaa gcgactgaac atgtgccctt catttttcag
     1441 ttttatcttt aacaaactct cactcatatg caggcgtaac gcaatcgcac caagcgtcca
     1501 gcttgctgat atatctgtct gaattatcgc cctgactttg gcacttatac tggataaaca
     1561 tccacttaaa aataataata tccgttcatc tgattcaaac agcgacaggc atgccatcat
     1621 aagaaacata tccgtggtat ctccggaaaa tctctggctg gtaattaaag cctcagccaa
     1681 cgcagggttg ttgggttcca gtgacagata aagcggaacg tcagtcagat gagttcttgt
     1741 cagcttatgc tgaatttcca gatattgact tactatggaa tggtttatat cgaaaatttt
     1801 aactttgcca taatgcataa ggaaaagcgc cctgatgcat ttggtggcca gaa
//
LOCUS       DAZFDP010000374         1848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFDP010000374 DAZFDP010000000
VERSION     DAZFDP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /locus_tag="ACHJ1G_005480"
     CDS             complement(<1..153)
                     /locus_tag="ACHJ1G_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4767108.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..1735)
                     /locus_tag="ACHJ1G_005481"
     CDS             complement(143..1735)
                     /locus_tag="ACHJ1G_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767109.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYEQQIAESRRPEEDTTPRET"
     gene            complement(1732..>1848)
                     /gene="gpW"
                     /locus_tag="ACHJ1G_005482"
     CDS             complement(1732..>1848)
                     /gene="gpW"
                     /locus_tag="ACHJ1G_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4767110.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
BASE COUNT          410 a          597 c          444 g          397 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtatcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcactttcaa
      901 tcgtcgctgc atacatcgcc ttcactatgg ccgactgaag ctgtgttgcc tgcagggaat
      961 cgagcatctt cagccgttcc atgacgctgt aaaactggtt ggccccacgg gtctgcccgt
     1021 cctccaccgg ctcgaaaata tgcagcatgg ccggacgccc ggtgggaagt tcacgcggga
     1081 tccgttccca tcgtccactc ccggagaacg gaaaatcatc ctcacagata tggtacgcaa
     1141 cggcacggcc atatcgatcg acctccaccc cggcccgcag aaaacggttc cccataccgt
     1201 gtcctggcgt gtccacccgt ttcggactca cggctttaaa acgcgtacgg aataactgcg
     1261 tggtttccgt atcccagacc ggctgcacaa agatttcgcc gttaaacgca tgaacgccca
     1321 caccttcacg gataaattcc gtgaacgtgc gttttccttc cacgtcgatc tcgccagaca
     1381 tcccttcggc gtattccgac caggccgcct ccacctcatc gacaaagctt tttgctgcgg
     1441 tctcccgcat ccccagccag cgccagttcg gacggtagct gatcagaaac atatgcccga
     1501 caatgtgatc cttatgcaga gccaccgcat tagccgctat tccgttattg cgcaccagat
     1561 catctgcccg ggcattcccc agacgcaacg cgggcagcag ggccgcatcg gcactctgcg
     1621 ccggtggcaa ccactcagcc atttgcccgc caaatcctgc accgcccccg ttgtagctga
     1681 gactctcacg aagcggaacg ccgttcacat caatcaggac aggcgttcgt ttcataacct
     1741 cactcccagc ggacgacggc gacgccgggt tgtccccagt accgactccg catcattgat
     1801 cgcccggtta agctcatcca gagaagccgc cgtatattca attctgcg
//
LOCUS       DAZFDP010000375         1844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFDP010000375 DAZFDP010000000
VERSION     DAZFDP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..470
                     /locus_tag="ACHJ1G_005483"
     CDS             <1..470
                     /locus_tag="ACHJ1G_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4767111.1"
                     /translation="FQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIF
                     DKKQIYYRMDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAV
                     MLEYMKEKDVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(658..852)
                     /locus_tag="ACHJ1G_005484"
     CDS             complement(658..852)
                     /locus_tag="ACHJ1G_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4767112.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            1241..1786
                     /locus_tag="ACHJ1G_005485"
     CDS             1241..1786
                     /locus_tag="ACHJ1G_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4767113.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          459 a          394 c          494 g          497 t
ORIGIN      
        1 ccttccagat tgccagggca tttggtaaac gccatcagca tgtgattagg gctattaaat
       61 gtttgagatg ttctgaggaa ttctcgacaa cccatttttg ggccgtcgag aaaatcaatg
      121 acttaggtat ttttgacaag aaacagattt actaccgcat ggactttagt ggcttcgtta
      181 tgctggttat gggatttaac ggggcaaaag ccgatgctgt taaagaagcc tatatcaatg
      241 cgtttaactg gatgtcagca gaactccgta agtacagcga aagttatgaa gcagaacgta
      301 acgccgtaat gctggagtac atgaaagaga aggatgtcgc cagcatgtca ggccgtctgc
      361 tcaatcgctg ggggagaacg aaaaaacctc aattgcttgc aaagctggaa cgtctggaga
      421 gacagggaca gtttttatta ccgggattcg ataaaggtat tcaagcctga cacattatgc
      481 gctgtatcgt cgccgtattc ccgcattaac catgaccgta gcccgacggg gaattccttc
      541 tgcgtgagtg tgcgggaata atcaaaaacg atgcacaccg ggttttactg tgctgacaga
      601 cgcagggtta ccctcatagt cgcttttccg gtgcgatggt ggaagaaacc gggatgttca
      661 tccatcatca ctttggattg atgtatatgc tctcttttct gacgttagtc tccgacggca
      721 ggcttcaatg acccaggctg agaaattccc agaccctttt tgctcaagag cgatgttaat
      781 ttgttcaatc atttggttag gaaagcggat gttgcgggtt gttgttctgc gggttctgtt
      841 cttcgttgac atgaggttgc cctgtattca gtgtcgctga tttgtattgt ctgaagttgt
      901 ttttacgtta agttgatgca gatcaattaa tacgatacct gcgtcataat tgattatttg
      961 acgtggtttg atggcgtaga tgcacgttgt gacatgtaga tgataattat tatcattttg
     1021 cgggtccttt ccggcgatcc gacaggttac ggggcggcga cctcgcgggt tttcgctatt
     1081 tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gccgtaacct aatgttttta
     1141 tttaaaacac cccctgaaaa gaaaggaaac gacaggtgct gaaaacgagc tttttggcct
     1201 ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtca acaaaaagca
     1261 gctggctgac attttcggtg cgagtatccg taccattcag aactggcagg aacagggaat
     1321 gcccgttctg cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg ccgccgtcat
     1381 aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaagttga
     1441 agaactgcgg caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca
     1501 tcgacttacg cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga
     1561 agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag
     1621 tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca
     1681 tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg cgctggatga
     1741 actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg ctgcggcatt
     1801 ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acag
//
LOCUS       DAZFDP010000376         1842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFDP010000376 DAZFDP010000000
VERSION     DAZFDP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1322
                     /locus_tag="ACHJ1G_005486"
     CDS             <1..1322
                     /locus_tag="ACHJ1G_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4767114.1"
                     /translation="SDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQV
                     AYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSA
                     NIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNK
                     EEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNY
                     GIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINI
                     TNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESE
                     TELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGV
                     VGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1335..>1842
                     /locus_tag="ACHJ1G_005487"
     CDS             1335..>1842
                     /locus_tag="ACHJ1G_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4767115.1"
                     /translation="MKWLLFITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYN"
BASE COUNT          585 a          358 c          354 g          545 t
ORIGIN      
        1 tatccgataa ctataatgct ataactctgg gaaatggttg gaatacaccg ctgggtgcca
       61 tctcttttta cgctgcccga tcgagtagta agttaaataa tgataccaga cacgaaggta
      121 cgagctatca ggtcgcctat aataaatatc tagtacaaac agccactcga tttagcgtgg
      181 ccgcctggcg ttatgcttcg caggattaca ggacattcag cgaccatctt tacgaaaatg
      241 ataaacacta tcatcagagt gattatgatg atttttatga tattggcaga aaaaatagcc
      301 tttctgctaa tattatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcgc
      361 tttggcggaa ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca
      421 ataactggca acacattagt tatactttct ctgccagcca atcttatgat gaaaataata
      481 aagaagagga gcgttttaat ctgtttatct ccattccttt ctactggggg gatgatattg
      541 ccaaaacacg tcaccaaatt aacttatcga attcgacctc attttccaaa gatggctatt
      601 cctccaacaa tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta
      661 tatatgttaa tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga
      721 gaactcccat cgccataata gatggcagct atagtcattc taaaaacgcc tggcaaagtg
      781 gtggaagtat tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagt
      841 tatccgatac atttgcaatt ctggatgcgc ctgggttaga aggtgcacat attaatggac
      901 aaaaatacaa tcgaacaaac agcaaaggcc aggttgttta cgacctgata atacctcatc
      961 gtgaaaacca tctggtactt gatatagcaa acagtgaaag tgaaacagaa ttgcagggca
     1021 atcgtcaaat tattgcacct taccgtggag cagtttctta tgtgcagttt acaactgacc
     1081 aacgtaagcc ctggtatata caggcactgc gtcccgatgg ttcgccatta acctttggct
     1141 atgacgtact ggatctccag gaaaataata ttggagtcgt tggccagggt agtcgccttt
     1201 ttattcgcgt agatgaaatt cccactggca taaaagttgc tctcaatgat gaacagaact
     1261 tattctgtac tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt
     1321 aaaaggagtt tactatgaag tggcttttat ttatcacctt gagtctatat tcttttattg
     1381 tgcaaagtgc tccctgtgca ttgaccaatg tcggtgaaca acgtggaaca tatatactca
     1441 agccactgag tatgaaaggt aatctaaccg ccgaaaaaat tgaaaacacc ggaaatatca
     1501 ttgagtgtac agacttgcaa gagacaacca acgccattaa agttcatttt ttaacacaga
     1561 acgctttaag gtttaaagtc ggaagtcgtc attatattat taatttcccc tttgaatctg
     1621 acggtaaaaa aatagagctt ccagggaagt catcttataa tttagaagaa atgctatctg
     1681 cgataaattt caccattcca tattctatcg catctgtatc agatagttct aacaccgcag
     1741 acatttttcc tggaactcct tttcctttgt tgttcgaaat tgaagtgagt tcatgtacag
     1801 ggaataatac taattgtaat agcgtccgct ttaattataa tt
//
LOCUS       DAZFDP010000377         1802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFDP010000377 DAZFDP010000000
VERSION     DAZFDP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1271)
                     /locus_tag="ACHJ1G_005488"
     CDS             complement(<1..1271)
                     /locus_tag="ACHJ1G_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767116.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQANSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLDALSRRDAREDPFASAGKTVSS"
     gene            complement(1217..1438)
                     /locus_tag="ACHJ1G_005489"
     CDS             complement(1217..1438)
                     /locus_tag="ACHJ1G_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767117.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            complement(1483..>1802)
                     /locus_tag="ACHJ1G_005490"
     CDS             complement(1483..>1802)
                     /locus_tag="ACHJ1G_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767118.1"
                     /translation="LGGTFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREA
                     SLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTA
                     SGG"
BASE COUNT          362 a          588 c          454 g          398 t
ORIGIN      
        1 tgtgatgaaa ctgttttccc ggctgacgcg aacggatcct cacgggcatc acgacgggac
       61 agcgcgtcca gactgtagtt ctgctgctga agatacagtg catcaccgcc ggcaaggggc
      121 ggcaggttct cacgtttacg ggcctcattg ggcgtgagaa gcgtattttt caccgattca
      181 cccagcgttt tcatgcgccg ttcgctgtcc attctcagca gcgtggtgac gtcaaactcc
      241 gtgctctcgt tttcccctgt ttccagcgcc tcatccagta acagctcaat ggactcaatc
      301 agcgtctgca gacactggga ataatactgc tgctccagcg cctccacgtt gtcactggaa
      361 ggcggctggc caacgccaat cttgtaggcc gggacacgga acaccgaaca gacaatttca
      421 gccgtcatct tcagttgttc caccgtctgc gcatccaccg gtgaaaacgt cgtggggtta
      481 tatttcgccc cgttgctcag gatcgccgtt ttccccgcat tttcgccggt atacccgctg
      541 tcccagttgc tcttcagttt tttcgcattt tcttccgtta tactgccggg gatctcaatc
      601 accccggacg gcctgccgcc atttctgaaa aaagacgttg aatttgcctg aatatgatgc
      661 ccctgcgtgg ccgccagccc ggcagcatac accggcggca gccccacaag cggatgaaaa
      721 aaacagttaa accggtcgtg gatcacttcc ctggcaggca ccgtcaccgc ctccgtgatc
      781 ccgcagttcc ggtccggcgt gatgcggtag aacacctcgc cgtcatccgc caccagaggt
      841 tcaacccggc tccagtccag aatacgcagt tctttgatct gcccccggga gttacggatt
      901 ttcagcacca ccgtattgcc gtgacgcagt ttggcgttca gccacagttc aaaaaactgg
      961 atacgattct gctgtgcatt gggacgacga cagagacggg caatatcccc ctgccgtttt
     1021 tcacggcgga tcccctgtgt atcggtctgc atcaggcgca gtcgcatttt ggcgatatcc
     1081 tgggatatca gcgaaatgca agaaaacacc gcatgaaagg agaggacact ttccggatcg
     1141 gctttcacgc cctgctgcca ggcgccggca aaaggctcag ccaccgcctg aaacaggctg
     1201 gtccagccca cctcttttac atcacgtcct gatttctggt tttttcgggt tcgccgcaaa
     1261 aggttccaca ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca
     1321 ctgtgatgta ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat
     1381 cacggatatc cccggcccgg gcatcatgcg tgccctgcag atatcggatc tttgccataa
     1441 cctgttacgg gaggcgcacg cctcccgccc tcctcatcag actcagccac cggacgcagt
     1501 tccgtagttc acaccggtga tcaccgccac tgccgcggta cggcgacgac gccagttgat
     1561 ccagcgctcc gcacggatgg ccacgctgcc ggtctgaaac atggagacca gttccaccgg
     1621 tgacggtgtg ctgctgtcgc tggtcggttc agactgcatc tccagtgacg cttcacggga
     1681 catatccacc gccacaccgc cgtcatcagc cagataaata tccggtgcat tcaccagtac
     1741 cagctggtca cccacatact gggagacaat caccggcagc ccctggaagg tcccgcccag
     1801 ca
//
LOCUS       DAZFDP010000378         1792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFDP010000378 DAZFDP010000000
VERSION     DAZFDP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..582)
                     /locus_tag="ACHJ1G_005491"
     CDS             complement(1..582)
                     /locus_tag="ACHJ1G_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4767119.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(873..1448)
                     /locus_tag="ACHJ1G_005492"
     CDS             complement(873..1448)
                     /locus_tag="ACHJ1G_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4767120.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(1561..>1792)
                     /locus_tag="ACHJ1G_005493"
     CDS             complement(1561..>1792)
                     /locus_tag="ACHJ1G_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4767121.1"
                     /translation="CGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVITQVV
                     ILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          497 a          483 c          310 g          502 t
ORIGIN      
        1 tcaattaccc tttataacga agtttcctct ggtatcgtca taaatgcatt cttcatgttt
       61 tacaattatt gatgccgtta ttggttcccg ggtcagtggg tggggtaagc cttcaccagt
      121 caaacgagaa aatgcagcgg catcaaataa agtacataca tctgaaccat ctgaattctt
      181 cacaaaaata cctttttcag gctgctccag tgtaattgga cattgaagca cctcaccagg
      241 acagtgaagt ttttcaatat caacagtaaa ggtacattcc gatattttgg cctgcaaaga
      301 gttctcttgt cgctgcgcca ccattccagg aagcacgctt aacaccctgt ttctgagcgc
      361 atcaactcca tcattaaaac caatattcaa taattctgca taaaccagtc ccagcccttc
      421 actttctgct aaaaaacggc ctgtatctgg catataagaa actctaactc tctcccctcc
      481 caggagaaat cctccaatct gcccttccat tacctgctga cgcatttccg ccatctcatt
      541 cgcgaatgaa gtcgatgata tatatgatac taatccaggc attaccatat tccttcatac
      601 agataaatat ttcctctggg cttcataaca aacatctccc tgacatgaca acaaaaaccg
      661 gagccggact ccggtttttg tgaatccgtc ggctatttca tcccgccaat atttccccgt
      721 cagcacgcca gatttgcagc ggcctcacca ctcactgtat atgcttttta gctgcatcca
      781 gtacaccaat taccacatcc ttataccctg aacatctgta gcatttcata taagcaactg
      841 gcagttaaca tatagcttgg gtaatattta tttcacttat cctttatgac aaagtttcct
      901 ttggtttggt cataaataca ttgttcttga cttacaatca ttgatgacgt tattggttct
      961 cgtgttagcg ggtgcttccc accgtcgcga accaaatgag aaaaagaaac cgaatcaaat
     1021 aaggtacaaa ccaaagaacc ttctgaattt ttaacaaaca caccttcttc tggtttatcc
     1081 agtataattg ggcatcgaac cgcctcggat ggacactgaa ggctctccgt attaacagaa
     1141 aacttacatt gtgatatttt cccctcaata tcaccatgtg attgtgtgtt acctgaacct
     1201 gaaaccattc ttaacattat atccctgagc gcttcaggac caccattaag ccctgtaagt
     1261 aataatccag aaagcaaact gttattgccc ccactcgcca gaaagcgacc atccgtttca
     1321 ctatatgtga tacggaccgt ctgcccgcct actgtaattt ctccaccgcc actggcggcc
     1381 atttgagcac gaagaacctg cattccggtg gaaaatgaat tggatgcaat agctgatgtt
     1441 aacggcatga caaaactccc tatttaaata ttcattccaa acacagggag tcataacaaa
     1501 cagcccccat acatgacaac aaaaaccgga gccggactcc ggttttgtga agctgtcggg
     1561 ttacttcatc ccgccaatat tttcccacgt cccgtcagca cgcagaattt gcagcggtct
     1621 taccacacac tgtatctgct ttttatccgc atccagtatc accacctgcg tgattaccct
     1681 gtcctgctcc gggataatac cattctcatc ggactccagg atgtctgccg gccccagtcg
     1741 cagttgtgct gtaagcgact gcacgtgttc acggccatca tgctttccgc aa
//
LOCUS       DAZFDP010000379         1791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFDP010000379 DAZFDP010000000
VERSION     DAZFDP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..734
                     /gene="queE"
                     /locus_tag="ACHJ1G_005494"
     CDS             63..734
                     /gene="queE"
                     /locus_tag="ACHJ1G_005494"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4767122.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1074..1651
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1078..1102
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          453 a          429 c          452 g          457 t
ORIGIN      
        1 agacatgatt gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg
       61 ctatgcagta cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg
      121 gcgtccccgc catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca
      181 ccaaacacac ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga
      241 agaccaaaga gagtgataag tggggggcgg cgagcagtga agatttgctg gctgtcattg
      301 gtcggcaggg atataccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg
      361 atttgctgcc actgactgac ctgctcgaaa agaacggttt tagctgccag atcgaaacca
      421 gtggaactca tgaagtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga
      481 acatgcgcgg cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc
      541 atccagtggg gcgcgtacgt gatattgaag cactggatga actactggca acgctgaccg
      601 atgataaacc gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt
      661 tgtgcattga aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc
      721 taaatattgc ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt
      781 ctactcattt atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta
      841 atcattagca ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg
      901 cggtaaagtt tggtagattt tagtttgtat agagttattt taaatattta cctttttaat
      961 caatggatta agtgctcttt aacataatgg atgtgttgtt tgtgtgatac tataaagttg
     1021 gtagattgtg actggcttaa aaaatcatta attaataata ggttatgttt agagtgttcc
     1081 ccgcgccagc ggggataaac cgatctcctc atcctcccag ctaccgacag tagtgtgttc
     1141 cccgcgccag cggggataaa ccgcaagatc tgccgcgagt ggtttcaccc ggcaggtgtt
     1201 ccccgcgcca gcggggataa accggatatt tgggcgtaat accgcccgga tagtgtgtgt
     1261 tccccgcgcc agcggggata aaccgtggta cggaacatgc tgtctatatt tcgaccagtg
     1321 ttccccgcgc cagcggggat aaaccgccag tggagccgcc gcgaatttac acacgattgt
     1381 gttccccgcg ccagcgggga taaaccgtaa ggccgtcgcc ggatcagcct ggctatgccg
     1441 agctccccgc gccagcgggg ataaaccgtt cttgcgggtg ttgcaaatat tcttcacgta
     1501 gagctccccg cgccagcggg gataaaccgg agcctgacga gactactgag gccgttctgt
     1561 cgagttcccc gcgccagcgg ggataaaccg gacgccgccg ccgcgaagcc gtttccgatg
     1621 ttgagctccc cgcgccagcg gggataaacc accatataac ccgttatctc tttctcaagt
     1681 ttttatatta gcagtacttg taataagcaa catatccacg taacacctca tgttcaaaat
     1741 agttctccat gccagagagg ttcacaatta tcgatacaaa aaattaaatt t
//
LOCUS       DAZFDP010000380         1773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFDP010000380 DAZFDP010000000
VERSION     DAZFDP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..585
                     /locus_tag="ACHJ1G_005495"
     CDS             <1..585
                     /locus_tag="ACHJ1G_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO4767123.1"
                     /translation="LIKTEQTGKLLNRKITTTPTPAKLLSYRNADLIKENYITEKVLS
                     IFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGIMMGTPRRSA
                     DIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASVGSLLDKGAK
                     VYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(598..819)
                     /locus_tag="ACHJ1G_005496"
     CDS             complement(598..819)
                     /locus_tag="ACHJ1G_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767124.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(903..1283)
                     /locus_tag="ACHJ1G_005497"
     CDS             complement(903..1283)
                     /locus_tag="ACHJ1G_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767125.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1484..>1773)
                     /locus_tag="ACHJ1G_005498"
     CDS             complement(1484..>1773)
                     /locus_tag="ACHJ1G_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4767126.1"
                     /translation="KISQCKFPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAV
                     CTLFDVDALSRVVNDGSVHPLTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          593 a          369 c          302 g          509 t
ORIGIN      
        1 ctaataaaaa cagagcaaac aggaaaactg ttaaacagaa aaataaccac cactcccaca
       61 ccagcgaaac tattatcata tcgaaatgca gatttaataa aagaaaacta cattacagaa
      121 aaagttttat ctattttcaa tataaaacgg gatttcgttg ctgtaagaat ccaaagtaat
      181 cagtttactg atttaaaaaa caagacaatt cagggtcata aagacactgt cgcaaaagtc
      241 atagactggt ataacccaca aaaaaacgca tttggtatca tgatgggcac accaagacgt
      301 tctgctgata tagcaaagga agaatccaga aacgctctaa atttcatgat aatggaaaag
      361 gatacattta atgaaaagat acttaacagc aatgctaatt tacaaaaaaa atacggcaca
      421 acagaaaata gtagttgggt atcggcttct gttggttctc ttctggacaa aggagccaaa
      481 gtatatccag atatatcatg ctctctgaga ctgggagaac cattcattat tacacttcca
      541 gaaacagtca ggctggatgt taatatccac ccactcaaaa aatgatcctg aacgttttta
      601 tgcaggcaaa tgcgtccaca tcaaggcttc aaccttgtgc tctccccctg tcacaggata
      661 aatttgcctg taaaaatcaa actctccctg ctcattacca ataccaatca gttgaatgca
      721 gttgccatct acaataaatt tttccgctga cgtaaccaaa ataaaggctt ccggatgttc
      781 attaggaaag gtgttagcaa gagtacactg aatatccatc tccaacaaaa tctcctgatt
      841 attagcgtaa actcatcgac gaaatcgtta ctatcccctg ggcacatatg taccattgaa
      901 tactactgtt ctccagattc tgataatcca tggcgaacaa taagaacact acagccgctt
      961 tgctgattag ttagccataa cggtgaactt tgctggatag tttgagaaat atcatttcgc
     1021 cggaggctta tacactgatt actcaagctt acaactgcag gtgcatcaaa gcgctccgga
     1081 ctatcagtat cacgcatagc tgtaaacaga atataatcac taccagccat atcatattcc
     1141 gtttctttta gtcttaacaa gccatcctct cttgtaatat taactgtaat taaagatgtc
     1201 tcatcatgat gggcatagcg ctgtgtatta agaattaaat gctcttccag aaattttaca
     1261 gggttactac gtatttcatt cattatacac cgaagctctg aacgatgcgg caccagaaag
     1321 tgcccggtat tttgtgaaat aatactactt accttgattg ttttctctcc agctcatgaa
     1381 aaataactcg cgtattatat tgttttacca accacaggcc ccgaatgcct gtggtgtttt
     1441 tatataaatt atcggaagtt aatattgttt catttgaaac atcttaacta tcttttataa
     1501 taaagcttcc ccttgcaggg tcatatttac actcttctgg tttaacaatc attgatgggg
     1561 ttattggagc tcgtgtcaga gggtgaactg aaccgtcatt aaccacgcga gaaagtgcat
     1621 caacatcaaa taaagtgcat actgctgaac tatctgaatt tttgacaaac actccttctt
     1681 cgggtctctc tagtgtaatt ggacactgaa tagactctgg cgggcactgg aaatttcctg
     1741 aactaacagg aaacttacat tgagatattt tgt
//
LOCUS       DAZFDP010000381         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFDP010000381 DAZFDP010000000
VERSION     DAZFDP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..566
                     /locus_tag="ACHJ1G_005499"
     CDS             <1..566
                     /locus_tag="ACHJ1G_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767127.1"
                     /translation="SYQENNLNCYESHETLAKIFKSSASTIKRKINDLKEMGLLETSP
                     HPDPYISSLIYNALPLTDAHITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDDWD
                     TPLPWETEETPVSSSEKVANDNEEDVLKNFASLIIKQKNTRTGGASFIEFATSLAYKH
                     RLKLPNGIEAYFAKKHPKVYEDFDIPF"
     gene            578..856
                     /locus_tag="ACHJ1G_005500"
     CDS             578..856
                     /locus_tag="ACHJ1G_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767128.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFIYSTL"
     gene            989..1399
                     /locus_tag="ACHJ1G_005501"
     CDS             989..1399
                     /locus_tag="ACHJ1G_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767129.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPDFQILDNADHYGDF"
     gene            1401..1736
                     /locus_tag="ACHJ1G_005502"
     CDS             1401..1736
                     /locus_tag="ACHJ1G_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767130.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPASLNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGTITEE"
BASE COUNT          630 a          329 c          320 g          488 t
ORIGIN      
        1 ttagttatca ggaaaacaac cttaattgtt atgaaagtca cgaaacatta gctaagattt
       61 ttaagtcttc agcgtcaaca attaaacgta agataaacga tcttaaagag atgggattat
      121 tagaaacatc cccacatcct gatccgtata tttcatcttt gatttacaat gctctccctc
      181 tcacagatgc acatattact cccccaggag aatcatcact ctctgatctt tctgaggcag
      241 aagaagcaca ggaacaacct aaaggccata aacagccttc ttttgatgtt cttgatgatg
      301 attgggatac gcctttaccg tgggagacgg aagaaacacc tgtttcatca agtgaaaagg
      361 tagctaatga taatgaggag gatgtgttaa agaattttgc ctctcttatt atcaagcaga
      421 agaatactag aacaggagga gcatcattta tagaatttgc aacatcgtta gcctataagc
      481 acaggttaaa gctaccaaat ggtattgagg catattttgc taagaaacat cccaaagtat
      541 acgaagactt tgatattcca ttttaataag gagggttatg attcaatatt tagtaaaaaa
      601 tcaagtagac agaattcagt gtaacgacac aggaaaacgc atctacgaaa cacttgctta
      661 cctctataaa ggaaagccaa caccgctaaa atatagcgat gtgttacatc gagcagggtg
      721 ctctgaagat ggtttaaaat tctggctgaa acagctatca aacttcggtg ttatagaaat
      781 taaagaatta tctttctcta cattcaatct caaaagacta gataaagaaa tagaattcat
      841 ctattccact ctctaaaatc tctctatgta attaaaatat aaaaggaaaa cattattatg
      901 atagtagcta tgtattggtt attgttcttt ctcacgttag aaacatactt cggggtgttt
      961 tcctttaaaa gaaaaggaaa agtaaataat ggcaaaaaga aaaaacaacg ttgttaagaa
     1021 aatcggggat tcagcttctc ttctaagtaa acctaaatct attctaagac gcaaagactt
     1081 taaagagcta gttacgctca ccaagcaaaa tagcgcccct ggcgaatgga aaacagagat
     1141 catagaacat tctccctctg tgccttgcgg agatgaattt aacgccttac aagaaatctt
     1201 atcttcaaca cctggcgtat tctggaaacc tagaaaaaga aaagagtata tcgttgatag
     1261 ctctgattta cgcaaatacc agattttagg atttgaagac tataaccact acgttggtta
     1321 tctcgccaca aatggcctaa ataacttagt tccagacttc cagattctag ataatgctga
     1381 tcactatgga gacttttaat atgaacaaaa acacgtatga cacaatttac tcactaatta
     1441 attattatga ggatgattac cttctgcctt taaaccgcgc tgaacttgaa gcatacaaag
     1501 aaaacacacc agcgtcacta aatgaggcgt ttaaacactg ggatttagct gtgaatgcct
     1561 ttgagcacct ctctaagcgt gtagagatgc tctgtaagcg cgaaaacgca tacctgacag
     1621 ccgatcagat atgggagcta tcaaactgga tagaagatat cgaatctgat gttcgctatg
     1681 tgggagatgg acttgtcgaa ctcgctcaac gcttaggcac tatcacagaa gaataaaaaa
     1741 agcaacaaaa aagttgtaaa atatctt
//
LOCUS       DAZFDP010000382         1759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFDP010000382 DAZFDP010000000
VERSION     DAZFDP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..730
                     /gene="yiiX"
                     /locus_tag="ACHJ1G_005503"
     CDS             122..730
                     /gene="yiiX"
                     /locus_tag="ACHJ1G_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4767131.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            875..1550
                     /locus_tag="ACHJ1G_005504"
                     /pseudo
     CDS             875..1550
                     /locus_tag="ACHJ1G_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1637..>1759)
                     /locus_tag="ACHJ1G_005505"
     CDS             complement(1637..>1759)
                     /locus_tag="ACHJ1G_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO4767132.1"
                     /translation="FYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT          459 a          439 c          406 g          455 t
ORIGIN      
        1 tattgcaatt tattgaattt gcacgaactt gtaggccgga taaggcgttc acgccgcatc
       61 cggcataaac aacgagcacg ttgtctgcga cccaccgctt tttatacatg gacgtttaac
      121 tatgaaaaac aggctgctga tcctcagcct gctggtttct gtacctgcct ttgcctggca
      181 gccgcaaact ggcgacatca tctttcaaat ctctcgctca tcgcaaagta aagcgatcca
      241 actggcaacc cattccgatt acagccacac cggtatgctg gtgatgcgta acaaaaagcc
      301 ctacattttt gaagcggtcg gcccggtgaa atacaccccg ctcaagcagt ggattgccca
      361 tggtgaaaag ggcaaatacg tcgttcgccg cgttgaaggc ggactgagtg ttgaacaaca
      421 gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa ccgtatgatt tcagcttctc
      481 atggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtttacc agaacgcgct
      541 gggaatgcgc gtgggcgaac agcagaagtt gaaagagttt gatctcagca acccgctggt
      601 ccaggcgaag ctcaaagaac gctacggtaa aaatattccg cttgaggaaa cggtagtttc
      661 accacaagcc gttttcgacg cgccacaact caccacggtt gccaaagagt ggccgctgtt
      721 ttcgtggtaa tggatgatga aaaaaataat tcccgccgta gtctgtttgt tgctttgtag
      781 ttgcacgacc tttccgcaag ctgtcgctcc ggtaaacggt gatgcctcca attagttgaa
      841 ttaaggatat aatactcgct tttaaggttg tttcatggtc acagttaacc ttcattgccc
      901 ccgttgccag tccgttcagg tttaccgtca cgggcagaac ccaaaaagac atgatcgatt
      961 ccgcggccgt gactgtcacc gggtatttca gctcagttac tgttacgagc cccgtaaacc
     1021 gggagttaaa gaccaaatcg ctgaaatgac tttcaacggt gccggggttc gcgatactgc
     1081 ccgtacgctg aaaatcggca ttaatacagt catccgaact ttaaaaaact ctcgccacga
     1141 agaataacgt catccccggt cgctcatgct gatgtggcac tcatctgcaa acttagtcag
     1201 caatggagct ttgtcggcag taaagcgcga cagcactggc tctggtacgt gtacaacacg
     1261 aaaactggtg gtgttctggc ctacactttt ggcccccgga ctgacgaaac ctgtcgtgaa
     1321 ttgcgggcgc tgcttaccct tttaccatcg gcatgttgac cactgacgaa tggggtagtt
     1381 acgctagaga gttaccgaag gagaagcatc tgactggtaa gatttttact cagcacattg
     1441 agcgtaacaa tctgaccctg agaacccgaa tcaaacgtct ggcccgcaga accatctgtt
     1501 tctcgcgttc tgtagaactt catgaaaaag tcatcggtgc ttttatcgaa aatatatgtt
     1561 ctactaattg gagtcatcac ccttctgata agctttcctt ggatgattac cagactgaat
     1621 acacagccaa agatttttac ctcattctgc tcttttttct caaatgacat atattatatt
     1681 ttacatcata ttcataggca tatgacattc ccccgccccc cattatgtca ttgtgggttg
     1741 atgttgggct ttcataaaa
//
LOCUS       DAZFDP010000383         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFDP010000383 DAZFDP010000000
VERSION     DAZFDP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            269..556
                     /locus_tag="ACHJ1G_005506"
     CDS             269..556
                     /locus_tag="ACHJ1G_005506"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4767133.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            553..1209
                     /locus_tag="ACHJ1G_005507"
     CDS             553..1209
                     /locus_tag="ACHJ1G_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO4767134.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            1206..1610
                     /locus_tag="ACHJ1G_005508"
     CDS             1206..1610
                     /locus_tag="ACHJ1G_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767135.1"
                     /translation="MKIELISKKVERLVMTRLEGDSTAKKAIKTTVNLNNELYTNVKD
                     SKLFRVRYFASVTIEGRLEMDITYDFDFRSEDDFSHEMAKSYEVRSIAPNMAYPYIKT
                     YAEQIILMSNLGRFTLPYFDFLANPMETNSNK"
BASE COUNT          571 a          349 c          368 g          468 t
ORIGIN      
        1 atcaaccagg accacaggcc aataacgttg ccttatcatc aagtagcaaa ggtggagtat
       61 gtggctggta ttctgaagca atctcgccat ctggatgaca tcgaggcaag ggagtggctg
      121 aaaagttcgt gacttcatcg tcacatagct ggtaaccagt ggcctgaaga gacgtttggg
      181 tgatgtacat agcatttctg gataaaaata cagattccct ttatgggaaa tgaatctata
      241 attcccaaag agggaacaaa atcggattat gaaggtctta aacgtagaga agcttcacag
      301 ttttagccgg aagcacaatc aggccaaggg ggctttagac tcttggtatg atgaagtgat
      361 aagagaaaac tggaaaacga ctcaagacat acggaataga tttaattctg ccgacttcct
      421 tcctaacaac agggtaattt ttaatataaa aggcaataac tatcggctcg ttgtccaagt
      481 tgtttaccag gcaggaatgg tcatagttga aagagttgga actcatgcag agtacgacaa
      541 gtggaggctt aaatgaatcg aactagctgg cgcatcatta aaaatagtga agagcatgct
      601 gcagctatgg aaaggctcat tgaacttgcg tctagtgatt tacaacctgg aactgaagat
      661 tttgatgagt ttgaactact aggcttgctt atcgagcact atgagtcacg cgagttccct
      721 atggacaagc cagatcccat agaagcaatc aagttccgta tggatcaaca aggcctctct
      781 tatgccgata tgaaacaata cattggctca gcatctaaag tatctgaggt cttaaatcgt
      841 aagcgtccat taagtctttc aatgatccgt agactacatg acggacttgg aattcctgca
      901 gatatcttaa ttcaagatat gagcgcaatt gaatggagcc tagttgacgc agaggaagaa
      961 gaaacagcca tgactagcgt cattgctcgg tgtgagtcag ccgtcacatc accttctgct
     1021 tatttcgctg aaaaggctac agaatcttac ttttcaaaaa tgttgttcag cgcagtaagg
     1081 ggtaatggca aatgcaaaga aaaacggaat gttttttctt tgataagtaa cttgtcatca
     1141 agtttcacag cggctagcaa cctgaatgac gaaatgactt ctgacggaaa ttacttatta
     1201 ttaccatgaa aattgaactc attagcaaaa aagttgaacg cttggttatg acacggctag
     1261 aaggcgattc aacagcaaaa aaagccataa aaacaaccgt taatctaaat aatgaacttt
     1321 acactaatgt gaaggattca aagctattta gagtgagata ttttgcctcc gtaactattg
     1381 aaggcaggct tgagatggac atcacatacg actttgactt ccggtcagaa gatgattttt
     1441 ctcatgaaat ggcaaaatca tatgaggtaa ggtccattgc tcccaatatg gcatatccct
     1501 atataaaaac atatgccgag caaattatcc tcatgtcaaa ccttggtagg ttcactctgc
     1561 cttattttga tttcttggcc aatcccatgg aaacgaacag taataagtga aattccccac
     1621 ccggcctcag cgccgggttt tctttgcctc acgatcccca cacctaaaaa cacataacca
     1681 attgtatttg ttgaaaaata aatagataca acttgctaaa caacgcaatc cagatctccc
     1741 tcaaatctct ttattt
//
LOCUS       DAZFDP010000384         1752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFDP010000384 DAZFDP010000000
VERSION     DAZFDP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..397)
                     /locus_tag="ACHJ1G_005509"
     CDS             complement(77..397)
                     /locus_tag="ACHJ1G_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4767136.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            505..678
                     /locus_tag="ACHJ1G_005510"
     CDS             505..678
                     /locus_tag="ACHJ1G_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4767137.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            749..1672
                     /locus_tag="ACHJ1G_005511"
     CDS             749..1672
                     /locus_tag="ACHJ1G_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4767138.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
BASE COUNT          491 a          381 c          386 g          494 t
ORIGIN      
        1 atgcgcggca tggtggctaa ttttggcggt ttcctttcca tcaataaact ttcgcagtaa
       61 tggattatgc ccaccgtcag gtgggttttt tatttagtag ttctctcaac ttttcgttct
      121 gctctctgaa cttttcctgt atttcttttt gcatggcgat aacctgggct tgaagttgaa
      181 ctagcgcatc aacatttagc ggaaccgaga cgctgttaat tttatctgct atttccccga
      241 gtgtattttc tgcattcaag gcatcttcca gtatctgaac aatctctgag ttcatagagc
      301 gcccgtttcg tttggctcgt tcagctatag catctcgcat tccgtcagga aaacggaggt
      361 tgaacttgtc gtagtctttt acttgttttt cggccattgc aaatctctca aaaaaaatca
      421 tggtgccata ttgccatacg atttcaatag tggcattatg gccttcatgg tgtcactttg
      481 gcaccaaata aaggagatta gataatgcaa gatacacttt tcactgagcg caaaaatatc
      541 aaactcaacc ttcgccttcc atcacggctg aatgaagacc ttcgccgcct ggcggaaatg
      601 gactgtatat ctctgaactc tgcaattgtt cgtttgctgg caaaaggtgt tagggaagag
      661 gtggcgaatg gtcgctaaaa acagcgaagc ctcaatggct gcaaccattg aggcttctaa
      721 attaccagtt aaccacgaga aaactgatat gactagttta gcaattgcag atcgcacaat
      781 caatgttcca ttccacggaa caaatctctt tttggttgga attaacaatg agccttatgt
      841 tcctatgaag cctgttgttg aaggtatggg gatggtttgg gctgctcaat ttgttaagtt
      901 aaaacagagg tttgtcaaag gtatttcgga aatcgaaata ccttctgctg gcggtaaaca
      961 gttaatgaca tgtcttgcct ttcgtaaatt tgcggcttgg ctttcaagca ttcaaccaaa
     1021 caaagtccgc cctgaaatcc gcgacaaggt aatccagtat caggaagagt gtgacgatgt
     1081 gctctacgag tactggacta aaggccatgt gattaacccg cgcaaagcta aaaaggcatt
     1141 gccggggaaa atcaccactg aacagcagga agccattaaa caactcgtca tgagtcgcgg
     1201 tcagtctctg ccaaaagaaa aacaggcgaa ggcgatgatc accatgtggt cgtcactgaa
     1261 atctcatttt gggtgttcat acaaagaaat cagcgaggag cagtttaccg aagctctgtc
     1321 acttgctgct cgcgttccgc ttgaaggaga gttaatcggc aaacaagaga agaaaaccaa
     1381 cgagctttct gcaaaagaag caaacagcct tgtatggtta tgggattatg ctaaccgctc
     1441 acaggcatta ttccgcgaac tgtatccggc gctaaagcaa attcaatcga actattcctg
     1501 cagatgctac gactacggtc atgagttctc gtatgttatt ggaatggcga gagacgtttt
     1561 aatcaatcac acgcgagata ttgatattaa tgagccagac ggaccaacga atctttctgc
     1621 atggctaaga cttaagaaca aagaattacc tccttcactg catcactact aacagattgc
     1681 caacgaaatg acccagcttc ggctgggttt tttatcagga gttctcatgc tctatagcaa
     1741 tatattggcg ca
//
LOCUS       DAZFDP010000385         1728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFDP010000385 DAZFDP010000000
VERSION     DAZFDP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            205..>1728
                     /gene="tir"
                     /locus_tag="ACHJ1G_005512"
     CDS             205..>1728
                     /gene="tir"
                     /locus_tag="ACHJ1G_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO4767139.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     "
BASE COUNT          487 a          375 c          423 g          443 t
ORIGIN      
        1 tatatcgcat gattattttc atttaattac actgatgtgt atttttctgc attattttct
       61 tgcatcaaaa atcagactgt gatttatttg gtcgatgtta tgttcagccg tatatcgact
      121 acgtactgta taccattaca ttattatgta atagcaatat aaaggattat tatctattta
      181 cataaaataa aaaggagata ttttatgcct attggtaatc ttggccacaa tcccaatgtg
      241 agagctttaa ttccacctgc accgccatta ccttcacaaa ccgacggtgc aggaggtgcc
      301 cgtaatcagc tcattaactc aaatggcccg atggggtctc gtttgctatt tacgcctata
      361 aggaattctg ttgctgatgc tgctgattct cgtgccagtg atattcccgg acttcctaca
      421 aatccactgc gctttgctgc gtccgaggta tctttgcatg gtgcgcttga agttcttcat
      481 gataaagggg ggcttgatac tcttaactct gctattggat cttcgttatt ccgtgttgaa
      541 actcgggatg atggcagcca tgttgctatc gggcaaaaaa atggcctcga gaccactgtt
      601 gttttaagtg agcaagagtt ttctagctta cagtcccttg atcctgaagg taaaaacaaa
      661 tttgtattta ctggaggccg cggtggcgca gggcatgcta tggtcacggt tgcttcagat
      721 atcgccgaag cccgtcagag gataatagat aaattagaac caaaggatac aaaggagacg
      781 aaggagccag gggatccaaa tagtggcgag ggaaaaatca ttgaaattca tacctcaacc
      841 tcaacttcta gcctccgtgc agatcctaaa ctttggttgt cattggggac tattgctgca
      901 ggtctgatag ggatggctgc gacggggatt gcacaggctg ttgcgttgac tccagagccg
      961 gatgacccaa tcactaccga ccctgatgct gcagcaaaca cagctgaagc agcggcaaaa
     1021 gatcagttaa cgaaagaagc attccagaac ccagataacc agaaagttaa tatcgatgag
     1081 aacggaaatg caattccgtc cggggaacta aaagatgatg ttgttgcgca aatagcagaa
     1141 caagctaaag cggcgggtga acaggccaga caggaagcta ttgaaagtaa ttctcaggcg
     1201 cagcaaaaat atgatgaaca gcatgctaaa cgcgaacagg aaatgtctct ttcatcgggg
     1261 gttggctacg gtattagtgg tgcgctgatt cttggcgggg gaattggtgc cggtgttact
     1321 gctgctcttc atcggaaaaa ccaaccggca gaacaaacaa tcactacacg tacggtagtc
     1381 gataatcagc ctacgaataa cgcatctgcg cagggcaata ctgacacaag tgggccagaa
     1441 gagtccccgg cgagcagacg taattcgaat gccagcctcg catcgaacgg gtctgacacc
     1501 tccagcacgg gcacggtaga gaatccgtat gctgacgttg gaatgcccag aaatgattca
     1561 ctggctcgca tttcagagga acctatttat gatgaggtcg ctgcagatcc taattatagc
     1621 gtcattcaac atttttcagg gaacagccca gttaccggaa ggttagtggg aaccccaggg
     1681 caaggtatcc aaagtactta tgcgcttctg gcaagcagcg gcggattg
//
LOCUS       DAZFDP010000386         1725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFDP010000386 DAZFDP010000000
VERSION     DAZFDP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..85
                     /locus_tag="ACHJ1G_005513"
     CDS             2..85
                     /locus_tag="ACHJ1G_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767140.1"
                     /translation="MSPKINLCNSVEVLTNSFGTVISGRQV"
     gene            291..1574
                     /gene="gltA"
                     /locus_tag="ACHJ1G_005514"
     CDS             291..1574
                     /gene="gltA"
                     /locus_tag="ACHJ1G_005514"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4767141.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          461 a          413 c          394 g          457 t
ORIGIN      
        1 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
       61 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
      121 ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
      181 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
      241 aaatttaagt tccggcagtc ttacgtaata aggcgctaag gagaccttaa atggctgata
      301 caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca
      361 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
      421 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
      481 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
      541 tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
      601 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
      661 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
      721 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
      781 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
      841 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
      901 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
      961 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
     1021 cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
     1081 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
     1141 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
     1201 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
     1261 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
     1321 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
     1381 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
     1441 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta
     1501 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
     1561 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
     1621 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
     1681 attcatatat tctaaaaaaa aatatatcgg tataaattat aaaca
//
LOCUS       DAZFDP010000387         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFDP010000387 DAZFDP010000000
VERSION     DAZFDP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..456
                     /locus_tag="ACHJ1G_005515"
     CDS             <1..456
                     /locus_tag="ACHJ1G_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4767142.1"
                     /translation="LVDRWRELEGATAKIPQTFSEALRLAADLEDQKAELEKQLALAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGARRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..1061
                     /locus_tag="ACHJ1G_005516"
     CDS             456..1061
                     /locus_tag="ACHJ1G_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4767143.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTIEECKAIKAGYQQKLKDLRNSRSEAA"
     gene            1058..1252
                     /locus_tag="ACHJ1G_005517"
     CDS             1058..1252
                     /locus_tag="ACHJ1G_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO4767144.1"
                     /translation="MTFTVKTIPDMLLEAYGNQSEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            1245..1679
                     /locus_tag="ACHJ1G_005518"
     CDS             1245..1679
                     /locus_tag="ACHJ1G_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4767145.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT          523 a          379 c          465 g          356 t
ORIGIN      
        1 cttgttgacc gctggcgaga actcgaaggg gcaaccgcga aaataccaca aaccttttct
       61 gaggcattgc gccttgcggc cgaccttgaa gaccagaagg ctgaactgga gaaacagctt
      121 gctctcgcag cacctaaagt tgagtttgcc gatcgagttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaata caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgacaagaaa gctgctagat
      421 aacggaatgc ttaaagtaac aggggaggct gcttaatggc taaaccagcg cgaaggaaat
      481 gcaaaatctg taaggaatgg tttcacccgg cattctcaaa tcagtggtgg tgcagcccgg
      541 aacacggaac taaattagcg ctcgaacgac gaaataaaga acgcgaaaag gcggaaaaaa
      601 cagcagagaa gaaacgacga cgagaggagc agaaacagaa agataaaatt aagattcgaa
      661 aactcgcctt aaagccccgc agttactgga ttaaacaagc ccaacaagcc gtaaacgcct
      721 tcatcagaga aagagaccgc gacttaccat gtatctcgtg cggaacgctc acgtctgctc
      781 agtgggatgc cggacattac cggacaactg ctgcggcacc tcaactccga tttgatgaac
      841 gcaatattca caagcaatgc gtggtgtgca accaacataa aagcggaaat ctcgttccgt
      901 atcgcgtcga actgattaac cgcatcgggc aggaagcagt agacgaaatc gaatcaaacc
      961 ataaccgcca tcgctggact atcgaagagt gcaaggcgat caaggcaggg taccaacaga
     1021 aactcaaaga cctgcgaaac agcagaagtg aggccgcatg acgttcaccg taaaaaccat
     1081 tcctgacatg ctccttgagg catatggaaa tcagtccgag gtagcccgaa tactgaactg
     1141 caatcgtgcc acagtcagaa aatacattgg cgataaagaa gggaaaaagc acgccgtcgt
     1201 caatggcgtc cttatggttc atcgcggatg gggtaaagat actgatgcgt gatatccggc
     1261 aggttcttga gcgctggggg gcatgggcgg caaataacca tgaggatgtt acatggtcgc
     1321 ccattgctgc cggatttaag ggactgatcc ccgaaaaagt aaaatcacgt ccacagtgtt
     1381 gtgacgatga cgcgatgatt atatgcgggt gtatggctcg ccttaacagg aacaacagcg
     1441 atctgcatga cttgctggtt gattattacg tgttggggga gacgttcatg gcgctggcac
     1501 ggaaacatgg gtgctctgac acctgtatag gtaaacgcct tcacaaagcg gaggggattg
     1561 ttgaaggcat gctgatgatg ctgggagtga ggcttgagat ggatcggtat gttgagcgtg
     1621 aattgccggg agggagaacc tctgtatttt atcagcgaaa aaatagttta cgatcgtaaa
     1681 aatctgcata tcatgataag agtggttaca ttgccacgct gct
//
LOCUS       DAZFDP010000388         1710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFDP010000388 DAZFDP010000000
VERSION     DAZFDP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHJ1G_005519"
     CDS             <1..882
                     /locus_tag="ACHJ1G_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4767146.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
     gene            1173..1526
                     /locus_tag="ACHJ1G_005520"
                     /pseudo
     CDS             1173..1526
                     /locus_tag="ACHJ1G_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          465 a          403 c          411 g          431 t
ORIGIN      
        1 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagcaaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgctgtaa tgcgttacgc cgtacagtct ggcatcatca gatataaccc ggctctggat
      181 atggctggtg cattgaccac ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc
      241 ctgcctgaac ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccggcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatatgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccgcg taaaccaatc agtgaaaata ctgtaaataa agccctgagg
      601 gtgatggggt atgacacaac ccaggatgtc tgtggccatg gattccgggc gatggcgtgc
      661 agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat
      721 caggagcgta atggtgtacg tgctgcgtat atccataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt cttgatgcga accgggagaa ggggatcagt
      841 ccgtttgaat acgcaaatgt taacaatccg ctgagaaggt gacagaaggc tattcacaca
      901 catgctcttc ccatggggaa gagcaacctg ctccaataaa tttctttttt attccggctg
      961 atagcaccgc aatcacccgt tacctctaaa tagccactta gcattcggcg tataagttga
     1021 cagagaagga gtatctcagc aggagcttaa agtgtggttt taggcagaat ttgattgatc
     1081 ctccatttgg ggcatgtgac taatcgatat agcttcctga acggaaaaag tgttttccct
     1141 tccaatataa actattcgac aataatataa atcaattaca gaataaaact ctgtataaac
     1201 atgcaaaaat ggtaaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1261 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1321 tggcggttgg aagaaaaaat tatctcttct tcggctcaga caagggggga gaaagcgcgg
     1381 cgatcatcta cagtctgctg gtcacctgca aactgaacga agtggagccg gaggactggt
     1441 tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgtgtgcat gaactgctgc
     1501 cctggaactg ctcaactgta aaataatcct tacgctacgt acttctcggg gcgcttacga
     1561 atttttcacg tgccacagaa ttttgaacgc tttctcttac aatatttcaa tgtttctatc
     1621 agtattcgcc ggaagaagtc atcgaccaaa tcatcccagt cgtctcgcac cactgaccat
     1681 tcatggttga catgtggtgg aaatccgctt
//
LOCUS       DAZFDP010000389         1695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFDP010000389 DAZFDP010000000
VERSION     DAZFDP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(299..1327)
                     /locus_tag="ACHJ1G_005521"
     CDS             complement(299..1327)
                     /locus_tag="ACHJ1G_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4767147.1"
                     /translation="MIIGNESVDLAPLFSLHDSRNFLLTSLDFTDPVGVSSHKVAVSQ
                     LVETNESLFNKETSRFSSEHNVTKREQGKEFLVEIPYFLREDMIKPQDVQGKRKPGAT
                     VEETLTDIYAEYVAKHHIAFQRTKESVLAASLFSGRTYTPKTDDVLIQWGDLFSVVPM
                     TATIDASSTTPSMVFQQFDQMHTDVITKAQSQAAAVERIVVFCKPAAFNAIRYSAGMV
                     NAFQYASPTDDANIVYSVRSLLPGVTTFTIPGTNIDIVKVTDPLLLAHMTSDAVAVPK
                     FAKGSNVYQNIFGAPSSTFDLLNSGAAEYYSYAFSPARNDAVNVVTENSQMVVNHGVG
                     FTVQITVA"
     gene            complement(1346..1693)
                     /locus_tag="ACHJ1G_005522"
     CDS             complement(1346..1693)
                     /locus_tag="ACHJ1G_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767148.1"
                     /translation="MFPVRKSFTDIVSYATDEFGCVQSFTVDLAVDVECGDLITSDGA
                     EYVSGGDVLVVVSDAVSAGDAKTVNVLRSNAGLVCIKSDALNVEGAVKEAAIAALAAK
                     GFQMAGFNTVFTS"
BASE COUNT          487 a          381 c          384 g          443 t
ORIGIN      
        1 agcagccagt agtgaagatt ctttgacgta cagcagtatt agagatccct aaaaacttac
       61 acacttgaga gatattcaga atatcttccg gtaaggtgga aacagctttc aggatctcaa
      121 ctggattgtt gttttgttcg ctcatgattt ttctctcaca atattttaat agacggtata
      181 aacgaaaaaa gggcaaaaga gattgtttac tgtatttgcg ctacaggttt aataatccct
      241 tttgccctta ttgcctgttt tatttccagt cgcacaggaa acgactatta acgctttatt
      301 acgctactgt gatctgtaca gtgaagccta cgccgtggtt tacaaccatc tggctattct
      361 cagtaactac gtttacagcg tcgttacgtg ctgggctaaa cgcatagctg taatattcag
      421 ccgcgccaga attcagcagg tcgaacgttg aagaaggagc gccgaaaatg ttctgataga
      481 cgttagagcc tttagcgaac ttcggaacag caaccgcatc agaagtcata tgagccagca
      541 gcagcggatc agtaacttta acgatatcga tgttagtccc aggaatagtg aaggtagtca
      601 cacctggcag cagggagcgc acgctataaa cgatgtttgc gtcatcggta gggcttgcat
      661 actggaacgc gttaaccatg ccagcactgt aacggatagc gttgaaagca gcaggtttac
      721 agaatacaac gatacgttct acagcagccg cctggctttg tgctttagta ataacgtctg
      781 tatgcatctg atcgaattgc tggaacacca tagaaggcgt ggtgcttgat gcgtcgatgg
      841 ttgcagtcat cggaacaaca ctaaacagat cgccccactg gatcagcaca tcatcagttt
      901 taggggtgta agtgcgacca gagaacaggg aagcagccag cacgctttct ttagtacgct
      961 ggaaagcaat gtggtgctta gcaacatatt ccgcgtagat atctgtcaga gtttcttcaa
     1021 cggtagcgcc tggtttacgt ttgccttgca cgtcttgcgg cttaatcata tcttcacgca
     1081 ggaagtaggg gatctctact aaaaattctt taccctgttc acgtttagtc acgttatgtt
     1141 cagaagagaa acgagaggtt tctttgttga acagagattc gttagtttcc accagttggc
     1201 taacagccac tttatgagaa ctcacaccta ccggatcagt aaagtcgaga gaagttaaca
     1261 ggaagttgcg agagtcgtgc agactaaaca gcggagccag atcgacggat tcattaccaa
     1321 taatcattat ttttattcct ttatattaag atgtgaatac agtgttaaaa ccagccatct
     1381 ggaaaccttt tgcagccagg gcagcaatag cagcttcttt cacagcgcct tcaacattca
     1441 gagcatcgga cttgatgcaa accagtccgg cattgctacg cagcacgtta acggttttag
     1501 cgtcgccagc ggataccgca tcagacacaa ctacaagaac atcgccgccg cttacatatt
     1561 cagcgccgtc actggtaatc agatcgccgc attcaacatc aaccgccaga tcaacggtaa
     1621 aagattgcac acaaccgaac tcatcagttg catatgaaac gatatcggta aatgatttgc
     1681 gaactggaaa cagat
//
LOCUS       DAZFDP010000390         1682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFDP010000390 DAZFDP010000000
VERSION     DAZFDP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1559
                     /locus_tag="ACHJ1G_005523"
     CDS             <1..1559
                     /locus_tag="ACHJ1G_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4767149.1"
                     /translation="DTPRVVKGVRFSLRLTNGSGEDSRLVTTAITADTEHRFSGLPLG
                     EYTLTVRAINRYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQF
                     EFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVE
                     VSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQ
                     TVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQ
                     PLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFS
                     LTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFP
                     RNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLK
                     NGVEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVM
                     KKSTAGISIS"
BASE COUNT          429 a          418 c          490 g          345 t
ORIGIN      
        1 gggacacgcc gcgggtggtg aagggtgtgc gcttcagtct gcgcctgacc aacggaagcg
       61 gagaagacag ccgtctggtg accaccgcca tcactgcgga tacagagcat cgtttcagtg
      121 gtctgccgct cggggaatac accctgacag tcagggcaat taacaggtat ggccagcagg
      181 gcgaaccggc caccaccacc ttccggatta atgcgccagc aaaacccgcc accattgaac
      241 tgacgccggg gtattttcag ataacggcgg tcccgcgtct tgcggtgtat gacccgacgg
      301 tacagtttga gttctggttt tcggaggcaa aaatcgcaga cacatctcag gtggaaacct
      361 ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc cggcccgcac attaagcccg
      421 ggaaggattt ctggttttac gtgcgcagcg tcaacctggt ggggaaatct gcgtttgtgg
      481 aagtcagcgg gcagcccagc aatgatggtg aagggtatct ggaatttttc cgggaaaaaa
      541 taggaaaact gcatctggct caggggctat gggagctgat agacaacagc cagcttgcgg
      601 atgagatggc ggagatgaag accaccatca ccgaaacccg caatgaaatc acacagacgg
      661 tcagtaaaac gctggaagac cagagcgcca ccattcagca gatacagcgc gtgcagaagg
      721 acacaaatga tgacctggct gcgctgtaca tgctgaaggt tcaaaaaacg aaagacggca
      781 ttccctatgt ggccggtatt ggagcgggga ttgaggatac tgatggccag ccactgagca
      841 acatactgct gctggctgac cgtatcgcga tgataaatcc ggagaacggc aacagcactc
      901 cgttatttgt ggcgcagggg aatcagctgt tcatgaacga cgtgttcctg aagcgtctgt
      961 ttgcggcgag tatcacgtca tccggcaacc ccccgacgtt ttccctgacg ccggaaggga
     1021 agctgacagc caggaacgcg gatatcagcg gagcaattac cgcgaatacc ggcacgctca
     1081 ataatgtcac cattaacgag aactgtgtca tcagagggaa actgtctgca aaccagattg
     1141 aaggcgacct ggtgaagacg gtggggaaag cctttccccg gaataacagt tatgccagcg
     1201 ggacggtaac cgtcacagtt tacgatgacc agggattcga ccggcagatt atcattcccc
     1261 cggtgctgtt tcgcgggacg aaacaccaga atttcaacag cccgaatcag cagtcgtact
     1321 ggtattccac ctgtaagctg caggtgctga agaacggggt tgagattttc catgaaccgg
     1381 caacggatgt cagccgggtg ttctcatcgg tgatagatat gccggcaggg cggggtcatg
     1441 tcaccctgac gtttaatgtg tcgtcggccg gtgcgaacaa ctggacgccg acaacgtaca
     1501 tcagtgattt actggttgtg gtcatgaaaa aatccacggc agggatcagt atcagctgac
     1561 ggtttattaa cccggacggg cacctcagga ggtgcctttt ttattgactg aaaacaaaga
     1621 ggtaatcatg cggcatttat acgcaacgat attattgttt actaccctgc tggcaggaat
     1681 tg
//
LOCUS       DAZFDP010000391         1682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFDP010000391 DAZFDP010000000
VERSION     DAZFDP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..318
                     /gene="essD"
                     /locus_tag="ACHJ1G_005524"
     CDS             103..318
                     /gene="essD"
                     /locus_tag="ACHJ1G_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO4767150.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            323..634
                     /gene="ydfR"
                     /locus_tag="ACHJ1G_005525"
     CDS             323..634
                     /gene="ydfR"
                     /locus_tag="ACHJ1G_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO4767151.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            631..1164
                     /gene="rrrQ"
                     /locus_tag="ACHJ1G_005526"
     CDS             631..1164
                     /gene="rrrQ"
                     /locus_tag="ACHJ1G_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4767152.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1161..1658
                     /gene="rzpQ"
                     /locus_tag="ACHJ1G_005527"
     CDS             1161..1658
                     /gene="rzpQ"
                     /locus_tag="ACHJ1G_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO4767153.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          482 a          346 c          438 g          416 t
ORIGIN      
        1 ttctttagcg ccgttgagaa caacgtgtgc tgtcaaaact accccgtaga ctccgatctt
       61 tttcaaacat attgcaccat ccgtgtacat cggggtgagg atatgaaatc aatggataag
      121 ttaacaacag gtgttgccta tggcacatcg gcgggtaatg ctggtttctg ggcattacag
      181 ttactcgata aagtaactcc gtcacagtgg gctgcaatcg gtgtgctggg tagcctggtt
      241 tttggcctgc tgacgtatct gacaaatctt tatttcaaga ttaaagaaga caggcgtaag
      301 gctgcgagag gagagtaatc caatgactca agactatgaa ctggttgtga aaggagtccg
      361 taattttgag aataaagtta cggtaactgt agccttacag gacaaggaac gctttgacgg
      421 tgaaattttt gacctggatg tcgccatgga ccgtgttgaa ggagctgcgc tggagtttta
      481 tgaggcagca gccagaagga gcgtccggca agtcttcctg gaagtagcag aaaaattgtc
      541 agaaaaagtt gagtcttatc tgcagcatca gtactccttt aagattgaaa atcctgccaa
      601 taagcacgag cgtcctcatc ataaatatat atgaacacaa aaatcagata cggcctgtcg
      661 gctgccgttc tggcgctgat tggtgctggc gcatctgctc ctcagatact tgaccagttt
      721 ctggacgaaa aagaaggtaa ccacacaatg gcataccgcg atggttctgg catatggacc
      781 atctgtcggg gtgccacagt ggtggatgga aaaaccgttt ttcccaatat gaaactgtcg
      841 aaggaaaaat gcgaccaggt caacgccatt gagcgtgata aggcgctggc atgggtggag
      901 cgcaatatta aagtaccact gaccgaacca caaaaagcgg gtatcgcgtc attttgtccc
      961 tataacattg gccccggtaa gtgtttcccg tcgacgtttt ataagcggct gaatgctggt
     1021 gatcgtaaag gtgcatgcga agcgattcgc tggtggatta aggatggcgg acgcgattgc
     1081 cgcattcgtt caaataactg ttacggtcag gttattcgtc gtgaccagga gagcgcatta
     1141 acctgctggg ggatagaaca gtgaatcaga tattcatggt gatttttctc gtgttgtcag
     1201 gatttatcgt cggaaatgtc tggagcgaca gaggatggca aaaaaaatgg gcggaacgtg
     1261 atgctgccgc attatcacaa gaggtaaatg ctcaatttgc tgctcgaata attgaacagg
     1321 ggcgaactat agcccgtgat gaggctgtta aagatgcaca acagaaatct gctgaaattt
     1381 ctgccagggc tgcttatctg tctgatagtg ttaaccagtt gcgtgccgaa gcaaaaaaat
     1441 atgccatacg ccttgacgca gcgaagcata ccgcagatct tgccgctgcc gtcagaggca
     1501 aaacaaccaa aaccgccgaa ggaatgctca ccaacatgct cggagatatt gcagcagaag
     1561 ctcagcttta tgctgaaatt gctgacgaac gctacatcgc aggagtgact tgtcaacaga
     1621 tctatgaatc tttaagagat aaaaagcatc aaatgtaggg taatattaaa tcggaacatt
     1681 ta
//
LOCUS       DAZFDP010000392         1671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFDP010000392 DAZFDP010000000
VERSION     DAZFDP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..202
                     /locus_tag="ACHJ1G_005528"
     CDS             <1..202
                     /locus_tag="ACHJ1G_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767154.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
     gene            210..656
                     /locus_tag="ACHJ1G_005529"
     CDS             210..656
                     /locus_tag="ACHJ1G_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4767155.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            653..1180
                     /locus_tag="ACHJ1G_005530"
     CDS             653..1180
                     /locus_tag="ACHJ1G_005530"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4767156.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1177..1359
                     /locus_tag="ACHJ1G_005531"
     CDS             1177..1359
                     /locus_tag="ACHJ1G_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4767157.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          448 a          349 c          461 g          413 t
ORIGIN      
        1 tgagttgatt ttttctgcat tgaggattct cggtgctatg tggatggtgg cgacgttcat
       61 tgtggttgcc agcagttttg tccggctggt aggcgaaggt aaagacctgg ttggtgtgct
      121 tttcggtagc attttcctgt gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaggtgacaa tgaagcaaat atacatgctt cgcaacgaag
      241 caatcagaaa caacgccata gacgcaatac tctcacttcc gatcgacgac aagtcacctc
      301 acgaagtcca cattaaagaa ccaaagcgga gcaatcctca aaaccgcctt atgtgggcgt
      361 tattgcagga cgtatcacgt caggtgcttt ggcatggaca gagacttgcg ccggaggact
      421 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgtgc
      481 ctggtatcga tggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      541 gcatgactga gcttatcgaa atcatgtttt ggttcggctc agagcgcaac gtgcggtgga
      601 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa aggtagggct gcatgactat
      661 caaatcaaat acaccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
      721 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaatgctct
      781 gtgcgctcac tggctaactg aggccgacga cgcactcaat tctgagtgga taagccacgg
      841 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
      901 gcagtgcata caacagcgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
      961 atggttcagc aaggctctgg agagtgttga cgaagttcgc attatcactg atggacggat
     1021 taattttatc gaaccatcga cagggctgga gaagaaggga aacagtaaag gctccatgct
     1081 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     1141 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtttc
     1201 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctacag aaataaacgc
     1261 aagccaatcc caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat
     1321 atccggtggc taagacatcg tgcgaggaaa acaaggtaat tgactaaaat cgaagttacg
     1381 aacaagaaag cgtcgagcgg gcttcagtgt acactgagtg gattctatct aggcttagtg
     1441 catacagaag attgctggta aaggacatgc caggcaaaac gatgaggact gatatttatg
     1501 aaaacatctg attttttact gttcttgcat gcggtacagg aggggctttg accgggcatt
     1561 ttatcgtgaa tattttcact tggtatttct ttggttttag agattacttc actcgatggg
     1621 ttttaaatag ttttcgtcgg tttatcgggt gcaagcctga tatgagaatt t
//
LOCUS       DAZFDP010000393         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFDP010000393 DAZFDP010000000
VERSION     DAZFDP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1287
                     /locus_tag="ACHJ1G_005532"
     CDS             <1..1287
                     /locus_tag="ACHJ1G_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4767158.1"
                     /translation="GSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVI
                     VVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCHP
                     KIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDEE
                     SSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRNI
                     AFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPVI
                     SAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIAL
                     NQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDE
                     LKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          455 a          295 c          385 g          480 t
ORIGIN      
        1 ggtagcagca cgcattctat tattaactct atcggtaagg gggctcttct tgattatgtg
       61 attattgatg aagcctcaca gcaggatatt gttccgggca ttctgggact gggatgtgcc
      121 cgtaatgtaa tcgttgtagg ggatcgaaag caacttccac atgttcctgt attactcccg
      181 aacagcccat ctccaccggc agaatactat aattgtgaga aatatagtct acttgattcg
      241 gtttgtatgt tgttcaggaa tatggtgcca gtaactcttc ttaaagagca ttatcgctgt
      301 catcctaaaa tcattcaatt ttgtaataag cagttttatg ataatgctct gataccgcta
      361 acgcttgatt caggtgaagc ctcattatct ctgatcatca cggcaaaagg aaaccatacc
      421 cgtaattttt caaatctcag ggagcttgaa tctctggaag gacattactg ggatgaggaa
      481 agtagcaggg gatatatcgc cccatataat gcacaggtta atctggctga aacagtgctg
      541 cctgctgatt ttgtgaaatc gacggtgcat aaatttcagg gacgggagtg cgatgaaatt
      601 gtgttttcca ctgtgctgga taagaagcgc agcagccagc acagtcggaa catcgcgttt
      661 gttgataatc ctgaactggt gaatgtcgct gtttcaaggg cccgaaataa atttacgctg
      721 gtgactggta atgatgtatt tgaacgccac gctggacata ttgcggcgct gattcgttac
      781 atcaaatact acgctgatga cggagagatt ttcgagagcc cggtcatatc agcatttgat
      841 ttgctgtaca gtgagtatga caaatcactg gaacgactga atagtagatt gaattcaaat
      901 gattcacact ttaagtcaga acagattgtg gcctgcctgt tgcgggatat cctgtcgcag
      961 gatagttatc ggagtatgat gtttcattca caaatagcgc tgaatcagtt ggtgttgctg
     1021 gagcgggggg attttacaca ccgtgaacag ttattcatgc gtaaccgggc cagttgtgac
     1081 tttgttgttt actataaggt cgggaagact ccattagggg ttattgaagt ggatggaggt
     1141 tatcatctga ccagtgttca ggctgaacgt gatgaactta aaaacagtat actgaagaaa
     1201 tgcggcctgc cattgctaag attacgtact atagacagtg atatagaagg gaagttggga
     1261 gcgtttctgt caggcctgac aggttaaagt ctgaataaaa aatattgcag cgggaggtct
     1321 caaacctgat ttggtcatgt gccacacaca attgtacgaa ccccatggac acaggcttct
     1381 ttgttttggt tacatcaacg tacgtattcc tgatacatat aaaaataagt gctattgctc
     1441 aaagctaaca gggaatggtt ctgttgaata attccttagt gaataaactt tatcaggggg
     1501 taattctatt acagtgaata cttcctgcaa aatgatattt gaattgttca ccatctgcat
     1561 catatttcct tatttacaag ttgaaaggat ggtatatgtt gaaaatgcat ggatt
//
LOCUS       DAZFDP010000394         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFDP010000394 DAZFDP010000000
VERSION     DAZFDP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1210
                     /gene="escC"
                     /locus_tag="ACHJ1G_005533"
     CDS             <1..1210
                     /gene="escC"
                     /locus_tag="ACHJ1G_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO4767159.1"
                     /translation="ITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPE
                     CIKYITSLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILK
                     TMASNGSLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELD
                     IEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNAN
                     NFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGT
                     LLRVTPRILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMT
                     TEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIK
                     ATPIKSASSE"
     gene            1224..>1587
                     /gene="cesD"
                     /locus_tag="ACHJ1G_005534"
     CDS             1224..>1587
                     /gene="cesD"
                     /locus_tag="ACHJ1G_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO4767160.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKK"
BASE COUNT          530 a          303 c          312 g          442 t
ORIGIN      
        1 aattacgcca acatatctgg atattgactc actgttaaaa tatctgcgtg atacaatctc
       61 agtaaataaa aactcctgta acgtgagaaa aatcacaaca tttaactcga ttgaagttcg
      121 tggcgttcct gaatgcataa aatacattac atctttatct gaaagtcttg ataaagaagc
      181 acagtctaaa gcaaaaaata aagatgtcgt tagagtattt aaattaaact atgcatcagc
      241 gacagatata acgtataaat accgcgacca aaatgttgtc gtgcctggcg tcgtctccat
      301 cctgaagacg atggcaagta atggctcact tcccagcaca ggcaaaggtg ctgtagaaag
      361 aaacggcaac ctgtttgata acagtgtaaa gatttccgca gatccacgtc ttaatgcagt
      421 tgttgtaaaa gatcgtgaaa taactatgga catatatcgg caattaattt ctgagcttga
      481 tattgagcaa aggcaaattg agatttctgt ttcaattatt gatgttgatg caaatgatct
      541 tcagcaactg ggagtaaact ggtccggtac attaaatgca gggcaaggtt ctattgcctt
      601 taacagctca acggcgcaag ctaatctcag ttcttctgtg ataagcaacg ccaataattt
      661 catgatcaga gtgaatgcgt tacaacagaa ctcgaaagca aaaatactct ctcagccatc
      721 aattataacc ttaaataata tgcaggcgat tcttgataaa aacgtaacgt tttatacaaa
      781 agtttccgga gaaaaggtgg catctctgga aagtattacc agcggaacat tactgagagt
      841 aacaccacgt atacttgatg acaactcgaa tagtcttacc ggaaaacgtc gggaacgagt
      901 tcgtttgtta cttgatattc aggatggcaa tcaatcgact aaccaaagta atgctcagga
      961 tgccagcagc atcttacctg aagtacaaaa ttcagaaatg acaacagaag cgacattaag
     1021 cgctggagaa agtctgctgc ttggcggatt tatccaggat aaagagagtt caagtaaaga
     1081 tggtataccg ttgttaagtg atattcctgt tattggtaac ttattctcca gtacaacaaa
     1141 acaaaaacac agcgttgttc gactcttttt gattaaagcg accccgataa aatcagcatc
     1201 tagcgaataa taaatgaggc tttatgagca ggaaatttag ctctctacag gatatctatg
     1261 atttctacca ggatggtggt acgttagcat cactgacaaa tttgacgcaa caagatctca
     1321 atgatcttca ttcttatgcc tatacagcat atcaatctgg tgatgttata accgcaagaa
     1381 atctatttca tctgctaaca tatctggagc actggaatta tgactacacc ctatctctgg
     1441 ggttgtgtca tcagcgttta tcaaatcatg aagatgcaca actgtgtttc gctcgctgtg
     1501 caactttagt tatgcaagat cccagggcat cttattattc tgggattagc tacttattgg
     1561 tcggaaatcg gaaaatggcc aaaaaag
//
LOCUS       DAZFDP010000395         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFDP010000395 DAZFDP010000000
VERSION     DAZFDP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..908)
                     /locus_tag="ACHJ1G_005535"
     CDS             complement(<1..908)
                     /locus_tag="ACHJ1G_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4767161.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHSDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPS"
     gene            complement(892..1248)
                     /locus_tag="ACHJ1G_005536"
     CDS             complement(892..1248)
                     /locus_tag="ACHJ1G_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO4767162.1"
                     /translation="MARPPKAPAYLNDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(1371..1553)
                     /locus_tag="ACHJ1G_005537"
     CDS             complement(1371..1553)
                     /locus_tag="ACHJ1G_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767163.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
BASE COUNT          315 a          439 c          399 g          420 t
ORIGIN      
        1 tgcgacggaa taccccgcgc tttctggatg gttgaggcca gtttttcacg atccacggaa
       61 acatcgatgt tagggttagc ctttatccac atcgccggat catcaacctc gttttcgtcg
      121 tccagctcgt aaatcagcac aaaaattgaa tcgttgacct cttcgccgtc caggatctgg
      181 cagcaataat catagtgctg tttacaggct gaaacgacgt tactgcccga tgtggtaatg
      241 gcaaataaca gcccttcggg acgtgcgccc atccccagct caagcgcgga ataaacgccg
      301 ttatcggagt gtaggtggta ttcgtccacg atggaaaggc ttgggtttgt gccctcgatg
      361 gttgccgctt ttgctgccag cggctttaac aggctgttgc ttttcgggtg catcaccttg
      421 tgggcctgaa tattcacccg cctgcgtaac ggtcgggata aaaggcacat ctgacgcgca
      481 tcatcaaaaa cgatccgcgc ctggtcacgg ctcaccgctg cggtgtaaat atcctgctgc
      541 ccgttctcca taatcagaaa ccagttagcc agaatcgcgg cggtcgtgga tttcgcattt
      601 ttgcgcggca cttcgataaa ggcgctcgtg tatttgcgcc gtccggtggc cttaacctta
      661 aagccgagga tgcacgcaaa ggcgaactgc tgccacggct ccagctcaat gggtctgcca
      721 cgcatcggcc cttttacgtg cgggcacacc ctggaaaagg caataaaccg ctccacaacc
      781 tcacgatcga acgtgtaaag ggggctttca aggtccgaaa agtaccgttt aacggcctgt
      841 ttcagccgtt tacaggccgg aattttgccc gtttttacgt cttctgcgta cttattccag
      901 gcggtcaagc tcgtcctctt cttctgtttc cggtggattt ttacggcggc ttatcgggtc
      961 aaaaccgagc aaggaggcca tttttatcat cactctttca gcgtcggatt ttgcgcttaa
     1021 tgcggggttt ctgctctcgc caccctgact gttaacaatg ctgaacccgc gcgccgcaag
     1081 gtctgcgacg gctttccggt atatcgagta attgacgcaa tacagttcca gattactcca
     1141 gtcggcgggg gtaaggtctc cccgttccgc aagctgccgc gatttttccc gccactgctt
     1201 cacggcgatg tcattcaggt aggcgggggc tttcggtggt cttgccatgc ttatttttcg
     1261 ccagattatt tttcaaaaaa ttcccgtgca taaaaatttg aggaggcgtt cggtgcccgg
     1321 cggggtcggg tttgtcctga aaacgccccc caccccgtca tacagcctca tcagcgattg
     1381 cggaaacatt ccataacctc gcggtcacgg tcggttaatc gcttcgctgt gatgcgttct
     1441 gcgcgtcctg acgctttatc tttatgccct gtttcctgtg tcttccatgc gtcacgctgc
     1501 cttataagtc cacggataag gcggttttgt tcccgctcat tcatcagcgt catacatcca
     1561 gttattgcgg tta
//
LOCUS       DAZFDP010000396         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFDP010000396 DAZFDP010000000
VERSION     DAZFDP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..206
                     /locus_tag="ACHJ1G_005538"
     tRNA            112..206
                     /locus_tag="ACHJ1G_005538"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:146..148,aa:Sec,seq:tca)
     gene            369..1538
                     /locus_tag="ACHJ1G_005539"
     CDS             369..1538
                     /locus_tag="ACHJ1G_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4767164.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYADKLYPN"
BASE COUNT          495 a          335 c          346 g          396 t
ORIGIN      
        1 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
       61 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
      121 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
      181 ggttcgactc ctgtgatctt ccgccaaacc tcatccagcc agttccgagg cactccataa
      241 aatcctttat attcaatgcg atgaataatc agcttatccg taatagtccg agatcatctc
      301 cctgaatccg cgaaaaatat gtatagtaat gtgtatagat tttttatacg cagttttcag
      361 ttatacacat ggctctaacc gatatacaga tcaaacgtgc aaaaccacaa gacaaaccat
      421 acacattgaa cgatggacaa ggtctgtcat tgcttatcaa tcctgatggc tcgaaaggct
      481 ggcgtttccg tttccggttt gcagggaaag cgcgtttaat gtcatttggc agctacgatt
      541 tagtaagcct cgcagaagca cgtgaaaagc gtgatatcgc ccgtaagcag gttgctaatg
      601 gcattgaccc ggtagaagaa cgtaaagctc agaaacttgc tcagaaatta tccactaaaa
      661 actcttttga gagcgttagc agagaatggc atgcagcaaa agcagatcgt tggaccttag
      721 cttatcgaga agaaattatt aaaacatttg aacaggatgt ttttcctttc attggtaaac
      781 gaccaatagc tgaaattaaa cccctagaac tgcttgaagt acttaaacgc atagaaaaac
      841 gtggagcgtt ggagaaaaca agaaaagtac gccaacggtg cggagaagtg tttcgctacg
      901 cgatcatcac tggccgcgct gaatacaatc cagcacctga tttagctatc gcattagcag
      961 tgccgaagca aaaacatcat ccttttctat cagcagagga actcccacat tttgtcagag
     1021 atcttgaggc atacactggc agcataatca ctaaaaatgc aaccaagatt gtgatgctta
     1081 caggtgttcg aacacaagag atgcgctttg ctacatggca agagcttgat ttagaaaaag
     1141 ggctatggga gataccagca gagcgtatga agatgcgtag gccacacata gtcccgcttt
     1201 caaggcaggt tataaggcta tttcaacaac taaaaccgat cactggtcac tatccttaca
     1261 ttttcattgg acgaaataac cgaaaaaagc ctataagtaa ggagagcata aaccaagtga
     1321 ttgagttatt gggatttaag gggagaatca ctggccatgg tttcagacac actatgtcaa
     1381 ccatactaca cgaagccggt tttaatagtg catggattga aatgcaactt gcccacgttg
     1441 ataagaacag cattcgcggt gtatacaatc atgcacaata tcttaatggt cgatctcgta
     1501 tgcttcagtt ttatgcagat aaactatacc caaactgata aataaggata aaataaaatg
     1561 tcttatttct tt
//
LOCUS       DAZFDP010000397         1562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFDP010000397 DAZFDP010000000
VERSION     DAZFDP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..338)
                     /locus_tag="ACHJ1G_005540"
                     /pseudo
     CDS             complement(1..338)
                     /locus_tag="ACHJ1G_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559742.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHJ1G_005541"
     CDS             complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHJ1G_005541"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO4767165.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1116..1376)
                     /locus_tag="ACHJ1G_005542"
     CDS             complement(1116..1376)
                     /locus_tag="ACHJ1G_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4767166.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1386..>1562)
                     /locus_tag="ACHJ1G_005543"
     CDS             complement(1386..>1562)
                     /locus_tag="ACHJ1G_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4767167.1"
                     /translation="FVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLS
                     KGLILQYFDLSINP"
BASE COUNT          539 a          324 c          273 g          426 t
ORIGIN      
        1 aacttaaatt taacagcttc agttgctaat tgcattttac tttgaaacca ggagacaagt
       61 attgttgtga acatgcaaac cagcaaaaaa ggaatgttcg ttttcaacca accaaccccc
      121 atagctttaa aatagtccca aatatctggt ttagcgttat gatcaaggat gtattcaaga
      181 atcgacataa tgtgatgcac atcaaaaaca tatccgagat ataaagtacc cacaagcatt
      241 attacagaga tggttaagtc tctactttat agaaattgcc cttttttaga agcatcctgg
      301 agtttctttt gtgttggctt ttcagtttta ttggccatga aacactctat ttctcaatgt
      361 atagttgcaa ttgttctggg aaaaaggata acggcattac tcgttcagcc agtattgggg
      421 caaaataaat aattaatatt aaaaatgcca gcgcactttt gacagtcagt gaaatggcaa
      481 aagcatttag ttgtgaagca taacgtgcca gcaatcccag gacagcctca ccgcccagca
      541 taacggcaat cacggggctt gcgtaaacga tagtatgagt cattatgtta ttaatatatg
      601 agaataaagg ctctaattta ggaaaattaa aattgcctga aggccataaa ttataacttt
      661 gccaaagagt ttccagaata aagttcattc cacctttagt aagataaacc gcagcagaga
      721 aaaggttaaa taatcttgct aattccgagg tatcgacacc tgtagccgga tccaaagtac
      781 tacttaatgt tgcaccgcgt tggttatcga taaagctacc tatagcaagg aaaatccaaa
      841 acggggatgc taataagcat gcaataaaca gccctatgat aatttctctg gcagcgaaca
      901 aagcaatatc gatttcattt aagttcaata attcatagtg gtaatgctga actaatgcac
      961 atgataccac tattattatt gggtgacgaa ctactgttgg tatattccca ctggcgaaaa
     1021 atggtaacaa atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca
     1081 gactatgcaa ctgatagagt atcgcctccc ccatgctatc ctctcgcgaa tgctaaattc
     1141 agcatttcga taccaaatga atataatttt tcaccatacc acccgctcat taaaaaaaag
     1201 caaatactta cgcacaataa tttcacgcca aatggcaacg tctgttcttg caactgcgtt
     1261 accgtttgaa aaagccctac caataaacca acaaatgtcg caactgcaat aggtcctgca
     1321 gacataacta aaatcagata cagcgcccta ttccccgcaa aaacaatatc gtccatattt
     1381 ttttctcagg gattaatgga taggtcaaaa tattgtaaaa tgagcccttt tgataacatt
     1441 gtccaaccgt ccatcgcgac aaacaaaatt aattttatcg gtgtagatat ggttaccgga
     1501 ctcatcatca tcatacctaa cgttaacaga acgctggata taaccagatc cactacgaca
     1561 aa
//
LOCUS       DAZFDP010000398         1561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFDP010000398 DAZFDP010000000
VERSION     DAZFDP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1158
                     /locus_tag="ACHJ1G_005544"
     CDS             <1..1158
                     /locus_tag="ACHJ1G_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767168.1"
                     /translation="VSSQLPDGASDGNKAISETEHDAVKAMFRGDTEKMTERELSIIR
                     ALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVTVDEVRPVLQAMVDD
                     AVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSH
                     IPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIHVP
                     RDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGL
                     WRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRG
                     VFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
     gene            1161..1487
                     /locus_tag="ACHJ1G_005545"
     CDS             1161..1487
                     /locus_tag="ACHJ1G_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4767169.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1497..>1561
                     /locus_tag="ACHJ1G_005546"
     CDS             1497..>1561
                     /locus_tag="ACHJ1G_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767170.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
BASE COUNT          351 a          363 c          519 g          328 t
ORIGIN      
        1 gtttcatcac agctgcctga cggcgcatct gacggtaata aggcaatcag tgaaacagag
       61 catgatgcgg tgaaagcgat gttcaggggg gatactgaga aaatgacgga acgggaactg
      121 tccattattc gtgcactggg agaagaattc tccacagtgc tggcggattt acagcgcaca
      181 tttgagggga agatggcctc gcaggcacaa gcgtttgaag agaaactgac ttccctgtcg
      241 gcggtattac agaagcatgt gacggtggat gaggtgcgtc cggttctgca ggcgatggtg
      301 gatgacgctg tgggggccat tccggtaccg cgtgatggtc gtgattatga tccggatgta
      361 ctgcagcagg cggtgaatga tgcggtcgca aatattccgc agccggcgga cggtaaaagt
      421 ctcaccccgg atgatgtgcg tccgatgctt gaacagatgg tgaaggaggc tgtaagccat
      481 atccctgttc cgcgcgacgg tcgtgactat gatcccgatg ttctgcagaa ggcggtgaat
      541 gatgcggtcg caaatattcc gcagccggca gacggtaaaa gtctcacccc ggatgatgtg
      601 cgtccgatgc ttgaacagat ggtgaaggag gcggtaagcc atattcatgt tccgcgtgat
      661 ggtcgtgact acgatccgga tgttctgcag aaggcggttc tggatgcggt gagtgccctg
      721 ccggctccgc aggacgggcg tgatgccacg gctctggaaa tactccccgc cattgacgat
      781 caaaaatcct ttccccgggg cacgtatgcc acacaccagg gcggactctg gcgggcgtat
      841 gaaaaaacgc acgggatgcg gggatgggaa tgcctggttg acggggtggc ggatattgac
      901 gtcagcatga cgggtgagcg gttgttctct gtggtggtcc ggcagagcag tggccagcgt
      961 acggaaaaaa cattttccct gccggtgatg ctctaccgcg gtgtgttcag agccggtgaa
     1021 acctaccacc ccggcgatac ggtgacgtgg gggggctcgc tgtggcactg caacagtatg
     1081 accgaagata aacccggaga agctcattca tcagcctgga ccctggctgc aaaacgtggg
     1141 cgggatgcag gaggctgaaa atgacggcat tactgacact ggaagagatc aaggcacatc
     1201 tgcgtgtcga ccatgacgcg gatgatgaca tgctgatgga caaggttcgt caggctaccg
     1261 ccgtgctgct ggcctacatt cagggcagcc gggataaagt gatccgtgag gacggtgaac
     1321 tgatcccggg cgaggcatta acccggatga agggggctgc catgcgactg accgggatgc
     1381 tgtaccggaa tccggatctt gcggagcggg aagaactgct tcagggggag ctgccgtttt
     1441 ctgtttccgt gctgatttac gatttgcgtt gtccgacggt gttatgagga gggggaatgg
     1501 caatatctgc aggtcgtctg acacagatga taagtgttct gaacccggtg ttaacccgta
     1561 a
//
LOCUS       DAZFDP010000399         1552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFDP010000399 DAZFDP010000000
VERSION     DAZFDP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(924..1400)
                     /locus_tag="ACHJ1G_005547"
     CDS             complement(924..1400)
                     /locus_tag="ACHJ1G_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767171.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          345 a          412 c          393 g          402 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctcctgcacg ttttttgccg tccgttcacc gttaaccccc
      241 gcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggatga
      481 gtcgtcagat agcccttcaa acgcgctcag aagctctcag acgcacgagg cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagtcactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcggt cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa cacgtagccc
      901 ctgattgtcc gcctgccgct ggctcaattt gaaaacatgg ccatgattcg gcgctgaata
      961 tcaccgtctt tgttgaccag tgttttagtg acaaatgctg catggttttt cgtagtgttg
     1021 tatcccttcc agacttcatg tggatcgagg gcatacgcac aaaccttacc agcaacgggg
     1081 attctaacta atgcattttt tgattctaag taattgagcc aacgaataat tgtgcttctg
     1141 ctaactccaa gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga
     1201 tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc
     1261 agcagctctc gaacgcgttc ccaacctttt ggggatacct gtgtaaacct tgaattcttc
     1321 tttgcttctt cgagatcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt
     1381 ttttctgaat caataaacat gattttttcc ctctaaaaat cccatgcact aacgttggtt
     1441 caaatctaag ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg
     1501 atgcgacaac gttgtgtcac cctggatgcg acaggtaaac aaaaaacaca ct
//
LOCUS       DAZFDP010000400         1546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFDP010000400 DAZFDP010000000
VERSION     DAZFDP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..356)
                     /locus_tag="ACHJ1G_005548"
     CDS             complement(<1..356)
                     /locus_tag="ACHJ1G_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4767172.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHJ1G_005549"
     CDS             484..1344
                     /locus_tag="ACHJ1G_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4767173.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          421 a          303 c          405 g          417 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcactgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaatg
//
LOCUS       DAZFDP010000401         1537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFDP010000401 DAZFDP010000000
VERSION     DAZFDP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..131
                     /locus_tag="ACHJ1G_005550"
     CDS             <1..131
                     /locus_tag="ACHJ1G_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4767174.1"
                     /translation="PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            144..518
                     /locus_tag="ACHJ1G_005551"
     CDS             144..518
                     /locus_tag="ACHJ1G_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4767175.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            515..1336
                     /locus_tag="ACHJ1G_005552"
     CDS             515..1336
                     /locus_tag="ACHJ1G_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4767176.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          393 a          311 c          439 g          394 t
ORIGIN      
        1 taccgctgtg tcgggagcat cacaacgagc ttcatgcgga tccgctggcg ttcgaagaaa
       61 agcatggttc ccaggttgat ttaatttttc gttttcttga tcacgccttt gcaaccggcg
      121 tgctcgggta aaagaggtta ctgatgcgta tagagtttgt tttgctttac ccgccgacgg
      181 tgaacaccta ctggcgacgt cgtggcagca catattttgt atcaaaagcc ggtgagcgtt
      241 atcgccgggc tgtggcgctt attgttcgcc agcagcggct gaaattaagc ctgtccggaa
      301 ggctggcgat aaagattatt gccgagccac cggataagcg ccgccgtgac ctggacaata
      361 ttctgaaagc gccgctggat gcgctgacgc atgcgggagt gttaatggac gatgagcagt
      421 ttgatgaaat caatatcgtt cgtggtcagc cagtatctgg tggacgtctg ggggtgaaga
      481 tttaccccat aatgcttgaa gggctggtca aaaaatgaaa ctggaagatt taccgaaata
      541 ctactcccca aaatcccccg gcctgactga tgcatcggcc tcaacgtcga aagatacgct
      601 gagtatcact gatgtgatgg ccgcgcaggg catgacacag aattgggctg agatggggtt
      661 ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg caacagaatt
      721 gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa aactcccggc
      781 agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag attatgcccg
      841 tagcgcggcg agcaaaaaac agtgcccctg ttgtcacgga aaaaaattta ttgaaagcga
      901 ggtttttaca aacaagatcc agtatccgga tggtaagccg ccagtgtggg caaagtgcac
      961 aaaaggcgtg tatccgtctt actgggagga atggaaaaaa gtcagggagg tggtaaaagt
     1021 tgcctgtccg gagtgtggag ggaaggggga ggtttccacc gcctgtaaag attgtcgtgg
     1081 gcgcggtgtt gccattcatc gtgaagagtc ggtaaaacgt ggtatgcctg ttatcagaga
     1141 ctgccagcgt tgtggtggtc gtggctatga aagattacct tcaacggagg catttaatgc
     1201 catatgtaat gtaaccgatg ccatatctct tgatacatgg aaaaaaacag ttaaacgttt
     1261 ttacgatacg ctggtggtgc agtttgatat tgaagaagca tgggcagaac aacaactgaa
     1321 aaaggtgacc agatagcttt gttgattttt cccgaatctg tggtaaattt gccctaacga
     1381 tgggcgtttt atgcctgacg ttagaagatt ttttacaccc gtcgccaggc gggttttttt
     1441 atgactgaaa tcacgccagt acagtaaacg cgctggtggt tgtgaatacc ggtctttcag
     1501 cttgctggct ttttcgacaa gagttattgg tgtgtca
//
LOCUS       DAZFDP010000402         1532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFDP010000402 DAZFDP010000000
VERSION     DAZFDP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..883
                     /locus_tag="ACHJ1G_005553"
     CDS             <1..883
                     /locus_tag="ACHJ1G_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767177.1"
                     /translation="IGFNFKDLPGPLKNALSNSNISTTAVSTIASTGTSATTSTVTTE
                     PKGPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGE
                     ADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGALGGFAVSRFAST
                     DPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGATLGATDLNKAEFEVARFTDY
                     PRIWWHAREGAIFPNKADIEHATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGLPHH
                     NPSNHVDIFDIIQETRV"
     gene            complement(1222..>1532)
                     /locus_tag="ACHJ1G_005554"
     CDS             complement(1222..>1532)
                     /locus_tag="ACHJ1G_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4767178.1"
                     /translation="SQESIQDKISQCKFPVSSGNFQCPPESIQCPITLERPEEGVFVK
                     NSDSSAVCCLFDFDAFSRLASEGSYHPLTREPITASMIISPDKCVYDPIKGNFIIKDS
                     "
BASE COUNT          493 a          311 c          295 g          433 t
ORIGIN      
        1 aataggtttt aattttaaag accttcctgg cccgttaaaa aatgcattaa gcaacagtaa
       61 tatatcaacc actgcagtgt cgactattgc atcgactgga acatcagcca ctacttcgac
      121 ggtaaccacc gagccaaaag gcccaatacc atggtttgga ttaacagctc aagtggttcg
      181 taatcatggt gtagaacttc ctatagtcaa aactgaaaat ggatggaagc ttgttggaga
      241 aacaccactt actcctgatg ggccgaaagc aaattacacg gaggagtggg ttatcagacc
      301 gggagaagca gattttaaat atggtgcatc tccattacag gcaactctag ggctggagtt
      361 tggcgcacat ttcaagtggg atttagataa ccctaatact aaatatgccg ttcttaccaa
      421 tgctgccgca aatgcgcttg gtgctttagg gggatttgca gtatccagat ttgctagtac
      481 agatccaatg ttaagtcctc atatcggtgc aatggttggg caagcagcag ggcatgccat
      541 acagtataat acccctggat taaagccaga cactatttta tggtgggctg gtgcgacact
      601 gggggctacc gatttaaaca aggccgagtt tgaagtagct agattcactg actatcctcg
      661 tatatggtgg cacgcaagag aaggagctat tttccccaat aaagcagata ttgaacatgc
      721 cacaggtgct gatatacgcg caatggaaga aggtatccct gttggacagc ggcatccaaa
      781 tccagaggat gtggtaatcg atatcgaaag caatggctta ccacatcata atccatcaaa
      841 tcatgttgat atctttgata taatccaaga aacaagagtc taagctttaa cttgtttatt
      901 ttaaaataat agctgaaatg gatggcagct attattaata aggattaata tatttttttc
      961 taaaataccc cacaaaataa acacatcata aaaattctaa atacacacct aatctcaaaa
     1021 aaaacaaact tactatttga cttggattat gtttttaatc caatattttc cgacaacatc
     1081 tccacagaaa aatgcattta cataaagacg ccttcaagtc atctgaaacc tgccaaccac
     1141 agacaccttc ttatattctg gatgtctgcg gcgttttttt acaaattact gacagccaat
     1201 actctctcag agaaaaatat tttaactatc ttttataatg aagtttccct tgataggatc
     1261 ataaacacat ttatcaggac ttataatcat tgatgccgtt attggttctc gggtcagtgg
     1321 atgatatgag ccttcactag ctaaacgaga aaatgcatca aaatcaaata agcagcatac
     1381 tgccgaacta tctgaatttt tgacaaacac tccttcttcg ggtctctcta gtgtaattgg
     1441 acactgaata gactctggcg ggcactggaa atttcctgaa ctaacaggaa acttacattg
     1501 agatattttg tcctgaatac tctcttgcga tt
//
LOCUS       DAZFDP010000403         1530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFDP010000403 DAZFDP010000000
VERSION     DAZFDP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1530
                     /locus_tag="ACHJ1G_005555"
     CDS             <1..>1530
                     /locus_tag="ACHJ1G_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4767179.1"
                     /translation="SVIGLVQYTRMIKTNDNISAIDHAGAVSDIKNIVDKFLGGILTF
                     TNNRVYNPGGISGASLEGFISSGLEVCASRIGGTAGRYLSNVAKVIKLPLLDIGINIW
                     SLYDSSLNHARATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEV
                     KNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIIDLSNNQVLGNVHLDMRE
                     NPPILHGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTKLSDAGEPDIFGWGDKDVC
                     NSMELSESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYE
                     IARAYTDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEHGMLGSNLHMIERFKNYNF
                     SISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDERKQNFYFHH
                     PSGFYSGKVMSITQKGINSVVGTKQGYDKIRGNNLDNTFSLGNGGGVIYSGGGSNTYF
                     V"
BASE COUNT          510 a          229 c          314 g          477 t
ORIGIN      
        1 tgtctgttat tggcttggtt caatatacac gaatgataaa aacaaatgac aatattagcg
       61 caatagatca tgcaggagca gtatcagata ttaaaaatat tgtagacaaa tttttaggcg
      121 gaattttaac ttttactaat aatcgcgtat ataaccctgg tggtatatct ggtgcctcgc
      181 ttgaaggatt tatatcttct ggtttagaag tgtgcgcgtc aagaataggt ggcaccgctg
      241 ggcgttatct tagcaatgtg gcaaaagtaa taaaattacc tttgctagat ataggcataa
      301 atatttggtc attatatgac tcttcattaa atcatgccag agccaccaca caaattgaat
      361 atatatcaac agcgatagat gtatcttttt catctataaa tactgcttta tcaataggcg
      421 caatcgcata tccacctttg gcaatagcta tagttccgat aactatcttt tctcatgaag
      481 taaaaaacta tgctgtttat gtgaatcaaa ttaatgagcg acataagtta tggcttgaag
      541 ctgaaaaata tctggacaat ggaagcgcaa aggttctaag cattaacaaa gatacaggca
      601 taatcgattt atcaaataat caggtgttag gtaatgttca tttagatatg agagaaaacc
      661 ctccgatatt acatggtgaa aaatcatata atagcgggaa gaatattgga agtcatccag
      721 atatgactga cagagaaatt atggagtctg gagcatacaa ttttgcatgt acaaaactct
      781 cagatgcagg tgaacccgat attttcggtt ggggagataa ggatgtatgt aattcaatgg
      841 aactgtctga atctcaatta gctaatgggt attctaaccg ccaatggcct tcacaaagtc
      901 cagttattcc tgaagggatt tataatacag tatatcttgg gtatggagaa acattgcgag
      961 ctaatacaga agttacattg agtagcactg gttactttta cgagattgca agggcatata
     1021 ctgatgatga actatctgag ccattattaa cagtatgtaa ccaacgtagt agtgtaattg
     1081 gtggaaaaga accattaaca ataatcattc cagctcttga acatggtatg cttggctcta
     1141 atctacatat gattgaacgt tttaaaaact acaatttttc aatatcagga gggaagggcg
     1201 ggataaaatt aatggttggt ggtattggtg attacaacat agaatgtacc cccggtgtta
     1261 gaaatattat ttcttttgaa caattatcac gtgacttcaa tctggatttg gatttatcag
     1321 atgagaggaa acaaaatttt tactttcatc atccctctgg tttttattca ggaaaagtta
     1381 tgagtataac gcaaaagggc ataaattcgg ttgttggtac taaacaggga tatgataaga
     1441 ttcgtggtaa taatttagat aatacctttt cccttggaaa tggggggggg gttatatatt
     1501 ctggtggagg aagcaataca tattttgttc
//
LOCUS       DAZFDP010000404         1515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFDP010000404 DAZFDP010000000
VERSION     DAZFDP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            796..1398
                     /gene="fimB"
                     /locus_tag="ACHJ1G_005556"
     CDS             796..1398
                     /gene="fimB"
                     /locus_tag="ACHJ1G_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4767180.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          458 a          266 c          322 g          469 t
ORIGIN      
        1 aaaataagtg tttacccgcc atcaggctga gcataattct catcatgaaa tatgtttcct
       61 ggtttgtggc ttgtagctgg tcactcctga agtctctctg gagaggcttg ttgatgttgg
      121 tgttttcagg atgatgtttc acttagcttg tttgccgaat agcccggcga acagctgtga
      181 ttgaggaagg tttaagtcgc agtgaccaaa gccatattta ccaacggatg cagatgaaaa
      241 aatcatctcc tgctttcccc catatctcca ggataaaaag gaatgtaaca atctcatgaa
      301 ctaaactgac gaaccagcca ggaataatcg ctagggacct aagaattagc ctgataatag
      361 ctactaagaa attactgcgt tccatgaaat agccattttg tggcaaatgg agttgtctaa
      421 taatgtcata tgtgagacgg atagttgaac gaatattaaa ttttgctgaa ttttttatgt
      481 tgattttact tgttacagaa catatcacat gatatataga taagattagt tgcattaatg
      541 atgagggtta ttattagatt cgtatccgat tgataaatat ataaaggtac atagcatgca
      601 agagcatagc gtttgtatgg caacgttatt ataagtaact gttgctactc catttaagtt
      661 cactcagaag aactggtcca cttacgttag ttattaagca aacgttcgct tttataaaca
      721 taatcaggat aaaaatgttg gattattgct aacccagcac agctagtgcg cgtctgtaat
      781 tataagggaa aaacgatgaa gaataaggct gataacaaaa aaaggaactt cctgacccat
      841 agtgaaatcg aatcactcct taaagcagca aataccgggc ctcatgcagc acgtaattat
      901 tgtctgactt tgctttgttt tattcatggt ttccgggcga gtgaaatttg tcgattgagg
      961 atttcggata ttgatcttaa ggcaaagtgt atatatatcc atcgattaaa aaaaggcttt
     1021 tcaacaacgc acccgctatt gaacaaagaa gttcaggctt taaaaaactg gttgagtatc
     1081 cgtacttcgt acccgcatgc tgagagcgaa tgggtatttt tatcgcgtaa gggaaatccg
     1141 ctttctcggc aacagtttta ccatattatc tcgacttccg gtggtaatgc cgggttgtca
     1201 ctggagattc atccgcacat gttacgccat tcgtgtggtt ttgctttggc gaatatggga
     1261 atagatacgc gacttatcca ggattatctt gggcatcgca atattcgtca tactgtctgg
     1321 tataccgcca gcaatgcagg gcgtttttac ggcatctggg atagagccag aggacgacag
     1381 cgtcacgctg ttttatagag atagttgaga taccagggat ggtgttaaaa actacattat
     1441 tcttacgata tatcaaaaat gattaaccat ttattaattg atgttattgt aagtgtgatt
     1501 attaattttc ataat
//
LOCUS       DAZFDP010000405         1508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFDP010000405 DAZFDP010000000
VERSION     DAZFDP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..258
                     /locus_tag="ACHJ1G_005557"
     CDS             <1..258
                     /locus_tag="ACHJ1G_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767181.1"
                     /translation="MTDERLFSVVIRQSSGQCTEKTFSLPVMLYRGVFRAGETYHPGD
                     TVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            255..581
                     /locus_tag="ACHJ1G_005558"
     CDS             255..581
                     /locus_tag="ACHJ1G_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4767182.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            592..942
                     /locus_tag="ACHJ1G_005559"
     CDS             592..942
                     /locus_tag="ACHJ1G_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4767183.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            939..1385
                     /locus_tag="ACHJ1G_005560"
     CDS             939..1385
                     /locus_tag="ACHJ1G_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4767184.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1382..>1508
                     /locus_tag="ACHJ1G_005561"
     CDS             1382..>1508
                     /locus_tag="ACHJ1G_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767185.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLI"
BASE COUNT          311 a          356 c          523 g          318 t
ORIGIN      
        1 atgacggatg agcggttgtt ctctgtggtg atccggcaga gcagtggcca gtgtacggaa
       61 aaaacatttt ccctgccggt gatgctctac cgcggtgtgt tcagagccgg tgaaacctac
      121 caccccggcg atacggtgac gtgggggggc tcgctgtggc actgcaacag tatgaccggt
      181 gataaacccg gagaagctca ttcatcaggc tggaccctgg ctgcaaaacg tggacgggat
      241 gcaggaggcg gaaaatgacg gcattactga cactggaaga gatcaaggca catctgcgtg
      301 ttgaccatga cgcggatgat gacatgctga tggacaaggt tcgtcaggct accgccgtgc
      361 tgctggccta cattcagggc agccgggata aagtgattcg tgaggacggt gagctgatcc
      421 cgggcgaggc attaacccgg atgaaggggg ctgccatgcg actgaccggg atgctgtacc
      481 ggaatccgga tcttgctgag cgggaagacc tcgtgcaggg ggaactgccg ttttctgtgt
      541 ccgtgctgat ttacgatttg cgttgtccga cggtgttatg aggagggagg aatggcaata
      601 tctgcaggtc gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaatgct
      661 gccggagaaa tgacggaaga atgggtgtca tgcgggaaaa ttcatgcgga tatccgtggc
      721 aggagcagcc gggagcggat gcagtctggt gcggaaatgg cgcaggcgga aatccgcatc
      781 tgggtgcgcg gtcagtccgg tcgggaaatc acggcagcgt cacgacttca tgtgctgagt
      841 ggtccctggc gtgaccggat cctgaacgtt gtcgggctgc ccgtgccgga tgcgaccggc
      901 ggacgtctgg aaattctctg tcggctggga ggtgaaaaat gatcgaaacc ctgctggatt
      961 tttcggggct ggaggacatc agccgcgatt tgcagcttct gagtggtgca gaaaataacc
     1021 gggtgctgcg tgaggcaacc cgtgcgggtg cgaatgtgct gaaagaagaa gtggtgtcac
     1081 gggcaccggt acgcagggga aaactgcgcc gcaatgtggt ggtcctttcc cggcgctccc
     1141 gcgatggcgg gatggaatcc ggtgtgcata tccggggtgt taatccggac accggtaaca
     1201 gcgataacac catgaaggcg gataacccgc gcaacgcttt ctactggcgg tttgtggaaa
     1261 tggggaccgt gaatatgcca ccgcacccgt ttgtgcgtcc ggcgtttgat gtgcgcagtg
     1321 aacaggcagc gcaagtggcg attgctcgga tgaaccgggc cattgatgag gtactgagac
     1381 gatgacggag gcggatttgt atcctcatct ggcgcatctt gccggcgggc aggtgtaccc
     1441 gtatgtggtc cccctgctgg atggcaggcc gtcggtggcg cttccgtggg tggttttcag
     1501 cctgattt
//
LOCUS       DAZFDP010000406         1507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFDP010000406 DAZFDP010000000
VERSION     DAZFDP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..86
                     /locus_tag="ACHJ1G_005562"
     CDS             <1..86
                     /locus_tag="ACHJ1G_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767186.1"
                     /translation="GNGWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            392..970
                     /locus_tag="ACHJ1G_005563"
     CDS             392..970
                     /locus_tag="ACHJ1G_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO4767187.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(930..>1507)
                     /locus_tag="ACHJ1G_005564"
     CDS             complement(930..>1507)
                     /locus_tag="ACHJ1G_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO4767188.1"
                     /translation="INSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
BASE COUNT          468 a          296 c          317 g          426 t
ORIGIN      
        1 agggcaatgg atggtttatt ggttcaatac atgacaccga agatggcccg gtttgtgtat
       61 ggctgagaaa taaggccgaa gcataaaggc tataaaccga ctaacaacta aatactgaag
      121 atttaaatca gaaacgattt ttattaaacc cttaaccgga gggattctgc accctcagaa
      181 catcaggagg ccgtctgaaa gggcggaaca gataatgctt acgttgaaac attttatcga
      241 cataccaaca tggttagccg tcattgcttt tgttaaaata cacatccact tttctgtgca
      301 atgtttaacc actggtcata tcaaatggca ttcatgcgaa ccatgatata gaatcatggc
      361 ttgagagagt cgatgaaagc gcaactatgg tatgagagac attgatgtaa gaaaggctgt
      421 gcatgccaag attctgagag atcatcataa agatcctgac accctaatca ttgatgagtt
      481 tacgatgaat ctaggggcta gcagagctga tatagcagtg atcaatgggc ttatacatgg
      541 ttatgagttg aagagcaaga gtgataactt gctcagatta ccagcgcagg tgcaacatta
      601 ctcatcagtg atggataaag taactttggt tgtctctgat tgccatcttt atgatgcttt
      661 aagcatagtt ccatcatggt gggggataaa gcaagttacg caaggtgcac ggcaaggtat
      721 ccatttaaaa acaattcgaa ctagcaagtt gaatccacaa gtggacaaac tttccttaac
      781 aatgcttctt tggaaagatg aattgctttc cctattaagt gatgtagggg agctacagaa
      841 tttgaaaaat aaacctaaac gcgtcttatg gtcaaaactc gccaatagta tggatgttgg
      901 cgagcttcgt gaagctgttc gagttaaact taaagcccgt aaagagtggc gagttgctca
      961 acaaccttag ttatgtgatg gttttgccca atcctacgcc atacctctgg gctaccaaat
     1021 ttatagttac cagagggatt ggctttgtag gcttgatact cgtttgcata atattctatg
     1081 tctctatctc ccgcacagaa tgtaggccct gaatattctc gatgagcaag aatatcctca
     1141 ctatgtttac catattgttc ataaccaaag cgattagcta ctcttcctcg aaatacccaa
     1201 aagtcattat ctccagagta tctgacgctg gcagatacgc tagggaatcg cgtcgaaagc
     1261 ctattaaagt cggggtgctg tactccataa tcactataaa tcacatttct ggcaagttct
     1321 tttctattca ttaaactctg ccataaaatc cactcgattc gaggttgaga atatagacca
     1381 acagaaatat cactgagatc tgtaggaaat gaacccccag aaagaatcac ttttctgtat
     1441 tcattgaggt gcgccagatt gtttatcaat cccattgcca aagtatatag ttcgccggaa
     1501 ttaattt
//
LOCUS       DAZFDP010000407         1505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFDP010000407 DAZFDP010000000
VERSION     DAZFDP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..502)
                     /locus_tag="ACHJ1G_005565"
     CDS             complement(<1..502)
                     /locus_tag="ACHJ1G_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4767189.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRI"
     gene            complement(502..843)
                     /locus_tag="ACHJ1G_005566"
     CDS             complement(502..843)
                     /locus_tag="ACHJ1G_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767190.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(836..>1505)
                     /locus_tag="ACHJ1G_005567"
     CDS             complement(836..>1505)
                     /locus_tag="ACHJ1G_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4767191.1"
                     /translation="QRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
BASE COUNT          305 a          546 c          335 g          319 t
ORIGIN      
        1 tgatgcgacc aggaaacagc gccccgtccg tctcggtcgg tgttgccagc acaaacgagg
       61 ctgtcatggc cgtcagctct gacatctgct ccaccaccca gcggtcgctc agctcctgct
      121 ccgggtccgc ttccggattg cccgccacaa aattcaccgc atccagaaaa cgggcataca
      181 cccggcggcg gaccaccgtg gcccccacca ggctctgcag gtcctccgcc atcccggtga
      241 ccagaccaaa cagattcgac accgtcagcg acgggcgggc actgctgccc ttcccgttca
      301 tctcaaagcc actgccgtca atcgggtatg cctggtactt ccgcccctgc caggtcaccg
      361 gctccccttt ttcattcagc tcattacaga aaaaataccg ctcaccaccc tgggccttca
      421 ggtcaatttc ccagagcacc acccgcggtg actgctctga tttaaccgac tcgttcaggc
      481 tttccccgtg aatatgctgc atcagttcac cacctgctca atcgtacagc tgaaatcact
      541 gtaccggacg ttatccgtga cgctccactc ccggcacacc accctcaccg tccggttatg
      601 tttcggcggt cgccacaaaa aggcacggta accaccatgc caggataaaa attcatccag
      661 ccagcgccgg gttgactcat ccgtcacccg gaacaccgcc tgaaacgtct tcagttgagg
      721 attcagtcct gtggggcgac gctgttcata accgtcacca aaccgtaccc tcgccaccga
      781 cggtttctca ctcacctgca tcccttcacg cgggaccaga tgcagcgttt ttatctcagc
      841 cactcagcat tcctccgtca cgtcgcatgg acaacatcac cgcctgcacc cgctggtcaa
      901 tcagctgcac aagactgcct gccgcctccg gccctatctg gccattagtc ccgtcattct
      961 gaatggcgat atggtagacc ggggaataca ccaggcccgc actaccgttc atactgccca
     1021 ccgctcgcac acccagcgag ccatccgccg cccgggtcag gggcataatg gcttcaggac
     1081 cggcctcccc catcagtccc gccccttttg caaaggcaaa gtacgtgggc gtatccacaa
     1141 tactgttgct gtacgcactc aggtttgccg aggtatacac gccgcctttt gcattggcca
     1201 ccgccccgcc cagccagtca ccaatgctgc cgagaaatcc tcccgcaccg gacataccgt
     1261 ttgccgccgt cttaattccg ttgacaatcg cggcattcat aagaactttt gatatttcct
     1321 gcagcactga tgaggcccag ctgcgccatt ccactttatt tccgttcagc atctccgtga
     1381 tgttattcac catccctgag ataccctccg tcgcaagctg tgctgcctgt gaggcgtaat
     1441 cggacgcatt atccacccag ttactgaatc cctcctgcag ccctttctgc cagtccgcac
     1501 gctgc
//
LOCUS       DAZFDP010000408         1498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFDP010000408 DAZFDP010000000
VERSION     DAZFDP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1496
                     /locus_tag="ACHJ1G_005568"
     CDS             <1..1496
                     /locus_tag="ACHJ1G_005568"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4767192.1"
                     /translation="IIYSNIQKVTSSRGNPLISRVPPDFFDLIIIDEAHHAPAQSWRD
                     TLHYFSNAKILHVTGTPYRGDNQEIPGVKIHETPLSEVMRDKYVKWLRKETVNAHDLF
                     FYTPEQPGVKLSKEQVLMFKDKEWIEKSIALSKECSLDVINHSIKKLNELKELSPNVP
                     HKILAIGCSISHAEDLLSWYKAKGMTSVIVHSEMDQNDLSDALMKIEHHQCDVVISVN
                     MLMEGYDHKYLTVLAIFRPYRSLNAFAQVVGRILRAIPESEITHFEIDNNGIVIFHEE
                     IGLNVMWETFQKEVERARRASVREYTFTDRDYHERSNTLAGIESDSAYVSDTDSYLDD
                     VDFNAMFEQKRNEINNSVEQQIEKIKSSGVDLPEDALEALRQTLAEKAVQKAAEIIDP
                     ELIEKRPHQARKLMRNILKTRTQDGVSNLLIDNGIGEKSSELYSIFNRHVPYLKTTDP
                     NDGILVRYVNLKLAKAYGPVDKRDNKTLLQSINDLDKILAEVERMIK"
BASE COUNT          545 a          247 c          284 g          422 t
ORIGIN      
        1 acataattta ttcaaacata caaaaagtaa catcttctcg aggcaaccca ctaataagtc
       61 gtgtaccccc tgactttttc gatctcatta ttattgatga ggctcatcat gcccccgcac
      121 aaagttggcg ggatactctt cattattttt ccaatgctaa gatattgcat gttactggca
      181 ctccatatag aggcgacaat caagaaatac caggagtaaa aatacatgaa actcctttat
      241 ctgaagttat gcgggataaa tatgtcaaat ggttaagaaa agaaaccgtc aatgcacatg
      301 atttattctt ttacacgcca gaacagccag gagtaaagtt atctaaagaa caagttttga
      361 tgtttaaaga taaagaatgg atagagaaaa gtatcgctct ttccaaagag tgctctttag
      421 atgttattaa tcatagtata aaaaaactta atgaactaaa agagctgtca ccaaatgtcc
      481 cacataaaat tttggctata ggctgtagta tatctcatgc tgaagattta ctttcatggt
      541 ataaagccaa aggaatgaca tcagtaattg ttcatagtga aatggatcaa aacgaccttt
      601 ctgatgctct aatgaaaata gaacatcatc aatgtgacgt cgttatatct gtcaacatgc
      661 tcatggaagg gtatgatcat aaatatctaa cagtacttgc tatatttaga ccataccgta
      721 gcctaaacgc ttttgcacaa gttgttggac gcattttacg agcgattcct gaatcagaaa
      781 ttacccactt tgaaatagat aacaatggaa tagtcatatt ccatgaggaa ataggtttaa
      841 atgtcatgtg ggaaacattt cagaaagaag tggagagagc acgcagagca agtgtaagag
      901 aatatacatt tacagacagg gattatcatg aaagatctaa tacattagct gggatagaaa
      961 gtgatagtgc ttacgtaagt gatacagact cttacctaga tgatgttgac tttaatgcta
     1021 tgtttgaaca gaagcgtaat gagatcaaca attctgttga gcaacaaata gaaaaaataa
     1081 aatcgtctgg agtagatctt cctgaagatg ctcttgaagc attacgccaa acattagcag
     1141 aaaaagctgt gcaaaaagcc gcagagataa ttgatcctga gctaattgaa aaaaggcctc
     1201 atcaagcgag aaagttaatg agaaacatct tgaaaacccg cactcaggat ggtgtttcta
     1261 atttattgat tgataatgga attggtgaaa aaagctccga actgtatagt atttttaacc
     1321 gacatgtacc ttatttaaag acaactgatc caaatgatgg tatattggtt agatatgtta
     1381 acctaaaatt agccaaagca tatggtcctg ttgataagcg ggataataaa acccttcttc
     1441 aatcaataaa tgatttggac aagattctcg cagaagtaga gaggatgatt aaatgaca
//
LOCUS       DAZFDP010000409         1475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFDP010000409 DAZFDP010000000
VERSION     DAZFDP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..683
                     /locus_tag="ACHJ1G_005569"
                     /pseudo
     CDS             1..683
                     /locus_tag="ACHJ1G_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(694..765)
                     /locus_tag="ACHJ1G_005570"
                     /pseudo
     CDS             complement(694..765)
                     /locus_tag="ACHJ1G_005570"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            828..974
                     /locus_tag="ACHJ1G_005571"
                     /pseudo
     CDS             828..974
                     /locus_tag="ACHJ1G_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1001..1105
                     /locus_tag="ACHJ1G_005572"
                     /pseudo
     CDS             1001..1105
                     /locus_tag="ACHJ1G_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          428 a          319 c          339 g          389 t
ORIGIN      
        1 atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa aagcactgcc
       61 ggacatcagc gctatctctg ctctccttgc cgtaaaacat ggcaactaca gttcacttac
      121 accgcctctc agcccggtaa gcaccagaaa atcattgata tggccatgaa tggcgtcgga
      181 tgtcgcgcca gtgcacgcat tatgggcgtt ggcctcaaca cggttttacg tcacttaaaa
      241 aaatcaggcc gcagtcggta acctcgcgca tacaaccggg cagtgatgtg attgtctgcg
      301 ctgaaatgga cgaacagtgg ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt
      361 acgcgtatga caggatacgg aggacggttg tggcgcacgt cttcggtgaa cgcactctgg
      421 ccacactgga gcgtcttctg agcctgctgt cggcctttga ggtcgtggta tagatgacgg
      481 atggctggcc gctgtatgaa tcacgcctga agggaaagct gcacgttatc agcaagcgtt
      541 acactcagcg cattgagcga cataatctga atctgagaca acatctggca aggctgggac
      601 ggaagttact gtcgttctca aaatcggtgg agttgcatga caaggtcatc gggcattatc
      661 tgaacataaa acactatcag taagttggag tcattacctc ctcccgaact caaagctatc
      721 atacagataa ttactggtta accctatata gggcatttta aagtgttgtg atcttaccca
      781 gcaatagtgg acacgcggct aagtgagtaa actctcagtc agaggtgatt cacaagacaa
      841 aacagtttca accagtaaaa aaacgtaaac accattcgca tgaatttcgt agttaagatc
      901 tgaagcttgc agaatgcatc ggtgttactg ccggagttcg tgaacttagt ctgcatgaat
      961 caccacagct ttacatctgt cgcagtaaac agcaaaatca gcaaacttac ttaattatat
     1021 cgagtgcgat tacaatcgct ggcgatggta cagttggtgt ggcggtctca gtccggaaca
     1081 atttgaaaat cagaatctca cttaaaacgg catccatttt acaaaagatg gccgtctgtg
     1141 tatttgtaat gaataccttc tacatgttta gagtgcataa tccacgacaa ggcgatatcc
     1201 taaaaattag ctagcaggca actttgatgg catagataat gtttttagta aaaaagagtt
     1261 gactgaatat aatacagcct cttcctacat aagaaggaat gtgctcacaa aaaaaaacca
     1321 ttagtctgct tcctgttctt acaataatca gtattatgta ttttatttat atagcaaaat
     1381 atgcttgtta cacctgtcat aattaggcat ggtaaaatta ttgactgaac cctgggggag
     1441 aaagatgcac agtgattatt ataatatggt ttttg
//
LOCUS       DAZFDP010000410         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFDP010000410 DAZFDP010000000
VERSION     DAZFDP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHJ1G_005573"
     CDS             complement(<1..270)
                     /locus_tag="ACHJ1G_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767193.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHJ1G_005574"
     CDS             complement(365..571)
                     /locus_tag="ACHJ1G_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4767194.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHJ1G_005575"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHJ1G_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          378 a          340 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tggtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZFDP010000411         1459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFDP010000411 DAZFDP010000000
VERSION     DAZFDP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1370)
                     /locus_tag="ACHJ1G_005576"
     CDS             complement(<1..1370)
                     /locus_tag="ACHJ1G_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4767195.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNR"
BASE COUNT          421 a          318 c          267 g          453 t
ORIGIN      
        1 cggttaaatt catccctgta tgtctctgcc gagaatgaat ttcttgttga aatatttctt
       61 cgaagatgtt gttttaaata aagcacagac catgatgtca ggcgctccag taaaatttta
      121 aaatcagatt cctttaatac ttcctcatat tcattcagag aaacttgttt ttcccggatg
      181 aatttttcat aataagaaag ctgaagagaa gtaaatagtg ccagccttct ctccggaatc
      241 accaatggtt tgactttcag tatcctgaag tttaacaaca aatcaattct gtttctgaat
      301 ccaatccgcc tgtcagaaag gtaatgaata taagccatca ggtcagttgt ttttcgtgag
      361 gaaagtcggt atttgtgaga aaaaagctcc tccggtcgaa gattctgact ctgccattgc
      421 tgcaggtatt tatactcaat attcagctca ttaatttctg cttttaacga agcgacctgg
      481 ttaatggcat tcaggtgttt tttcacttgt tgaagtacat catcaatcgc atttgcagac
      541 ggtgagtcag gtaagacttc ctcagaacgc gaaatcgacg ttgaaaaaaa ctgttgcctg
      601 ttctccgtac tccccagtcg tgccaccaca tatcccagtt gctgtttgtc catcttttca
      661 taaacattac tgacagctga gttattattg gacaaaattg ccactgtttt attctgaatt
      721 aatatattag caacaatatt gagtatagtc tgtgttttac ctgttccagg tggtccttca
      781 ataactgaaa cctgtgagga aaatgcccgt tcgacagcca gcaactgact ttcattaaga
      841 ccaaacggaa aaatcaaacc ccatggcgaa tcacgttttt tactctgtcc tgaacagtat
      901 gcatgcaagg cggtatccgg atgaggaact attttcttta tctgcctaag gatattctca
      961 tcgatatttc tgttattacc gcgggcataa aaaatacgtt cattagccac cctacacaag
     1021 taattaaaca cggcatgttc tgtaaatgct gaacactgtt gaaagtcaag accggtcgtt
     1081 ttcatgatgt agggtcgttc accctcgcga taataaacag cagcatattt atcaccatat
     1141 ataataacca gttcagcccg ggatgttaca gcgttccctt tcctttcgag gaaaacattt
     1201 cgcaaattta cagttggcgt tatctgacat acagaaagcg gacgagtata ttttctcccg
     1261 ctgcgaaaat gacaggtcag cataacctct ctgtcgtcat ttccagccca cagcgtccag
     1321 tcggtaattt tatgagtctt attttcaccg tcaacacaaa tcgtgaccat attcttactc
     1381 agaaattatc atttaactta cggaaaattg ttaaggcact cccgatatta gctccggagg
     1441 attttccaca actccggga
//
LOCUS       DAZFDP010000412         1457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFDP010000412 DAZFDP010000000
VERSION     DAZFDP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1180)
                     /locus_tag="ACHJ1G_005577"
     CDS             complement(<1..1180)
                     /locus_tag="ACHJ1G_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767196.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     Y"
     gene            complement(1126..1347)
                     /locus_tag="ACHJ1G_005578"
     CDS             complement(1126..1347)
                     /locus_tag="ACHJ1G_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767197.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          284 a          479 c          359 g          335 t
ORIGIN      
        1 gatacagtgc atcaccgccg gccaggggcg gcaggttctc ccgtttacgg gcctcattgg
       61 gcgtgagaag cgtatttttc accgcatccc ccagcgtttt catgcgccgc tcactgtcca
      121 ttctcagcag cgtggtgaca tcaaattctg tactctcgtt ttcccccgtt tccagcgcct
      181 catccagtaa cagttcaatg gactcaatca gcgtctgcag gcactgggaa taatactgct
      241 gctccagcgc ctccacgttg tcactggaag gcggttgtcc cacgccaatc ttgtaggccg
      301 ggacacggaa caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgcg
      361 catccacagg tgaaaacgtc gtggggttgt attttgcccc gttgctcaga atggccgttt
      421 tccccgcatt ttcgcctgta tacccgctgt cccagttgct cttcagtttt ttcgcatttt
      481 cttccgtaat actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa
      541 aagacgtcga attttcctga atatgatgcc cctgcgtggc cgccagcccg gcgacataca
      601 ccggcggcaa tcctataagc ggatgaaaaa aacagttaaa ccggtcgtgg atcacttccc
      661 gggcaggcac cgtcaccgcc tccgtgatcc cgcagttccg gtccggcgtg atgcggtaga
      721 acacctcgcc gtcatccgcc accagaggtt caacccggct ccagtccaga atacgcagtt
      781 ctttgatctg cccccgggca ttacggattt tcagcaccac cgtattgcca tgacgcagtt
      841 tggcgttcag ccacagttca aaaaactgga tgcggttctg ctgggcgttg ggacgacgac
      901 agaggcgggc aatatccccc cggcgcgttt ccctgcgtat cccatgcgca tccgtctgca
      961 taagacgcag ccgcattttg gcgatatcct gggatatcag cgaaatacat gcaaacaccg
     1021 catgaaagga gaggacggct tcaggatcgg ctttcacgcc ctgctgccag gcgccggaaa
     1081 agggctcagc caccgcctga aacaggctgg tccagcccgc ctctcttacg tcacgtcctg
     1141 atttctggtt ttttcgggtt cgccgtaaaa ggttccacat tcgccatgct ccgcatcacg
     1201 tttctttttc tgacctgccg gacgtcgcac cgtgatgtac tccgcctttc ccaggcgaac
     1261 cagcacctcc gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt
     1321 gccctgcaga tatcggatct ttgccataac ctgttacggg aggcgcacgc ctcccgtcct
     1381 ccttatcaga ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc
     1441 gccacaccgc cgtcatc
//
LOCUS       DAZFDP010000413         1441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFDP010000413 DAZFDP010000000
VERSION     DAZFDP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1347
                     /gene="rhsB"
                     /locus_tag="ACHJ1G_005579"
     CDS             <1..1347
                     /gene="rhsB"
                     /locus_tag="ACHJ1G_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4767198.1"
                     /translation="THQYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWR
                     RERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE
                     LAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCG
                     LTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEEN
                     PHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPI
                     SDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICED
                     KEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWD
                     EIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1319..>1441
                     /gene="yhhH"
                     /locus_tag="ACHJ1G_005580"
     CDS             1319..>1441
                     /gene="yhhH"
                     /locus_tag="ACHJ1G_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO4767199.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMR"
BASE COUNT          416 a          326 c          389 g          310 t
ORIGIN      
        1 acccaccagt accattacga cagtcagcac cggctggtgc actacacgcg gacacaatat
       61 gcagagccgc tggtcgaaag tcgctatctt tacgacccgc tgggccgcag ggtggcaaaa
      121 cgggtatggc gacgtgaacg ggacctgacg ggctggatgt cgctgtcacg gaaaccggaa
      181 gtgacctggt acggctggga cggcgaccgg ctgaccacga tacagaacga cagaacccgc
      241 atccagacga tttatcagcc ggggagcttc acgccactca tcagagttga aaccgccacc
      301 ggtgagctgg cgaaaacgca gcgccgcagc ctggcggata cccttcagca gtccggcggc
      361 gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga ccggctggaa
      421 agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc atcgtgcggc
      481 ctgactgtgg agcagatgca aaaccagatg gacccggtgt acacgccggc gcgaaaaatc
      541 cacctgtacc actgcgacca tcgcggcctg ccgctggccc ttatcagtaa ggaaggggca
      601 acagaatggt gcgcagaata cgatgagtgg ggcaacctgc tgaatgaaga gaacccgcat
      661 cagctgcagc agcttatccg cctgccgggg cagcagtatg atgaggagtc cggcctgtat
      721 tacaaccgcc accgctatta tgacccgctg caggggcgat atatcactca ggatccgatt
      781 ggactgaagg ggggatggaa cctgtatgga tatcaattga atccgatatc agacatcgac
      841 cccctgggtt tatctatgtg ggaggatgca aaatcggggg catgtactaa tggtctttgc
      901 ggcacactat ccgctatgat aggtccagat aaatttgatt ctatagatag caccgcatat
      961 gacgccttaa ataaaataaa tagccaatct atttgcgaag ataaagagtt cgctggttta
     1021 atatgtaagg ataatagtgg cagatatttc tcaacagcac ctaaccgagg agaaagaaaa
     1081 ggatcatatc cattcaatag cccttgccct aatggtactg agaaagtatc agcttatcat
     1141 actcatggtg cagatagtca tggagaatat tgggacgaaa tattttcagg taaagatgag
     1201 aaaatagtta aaagtaaaga taacaatatc aagtcatttt atttaggtac gcccagtggt
     1261 aattttaaag caatagataa ccacgggaag gaaataacaa acagaaaagg attacctaat
     1321 gtctgcagag ttcatggtaa tatgtaaaaa aatattgttt aggaactgtg tcattgtatc
     1381 tttgtttgtt tttacataca acacatgggc gcagtgtaat aataatatta aaataatgcg
     1441 c
//
LOCUS       DAZFDP010000414         1434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFDP010000414 DAZFDP010000000
VERSION     DAZFDP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..237
                     /locus_tag="ACHJ1G_005581"
                     /pseudo
     CDS             1..237
                     /locus_tag="ACHJ1G_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            449..599
                     /locus_tag="ACHJ1G_005582"
                     /pseudo
     CDS             449..599
                     /locus_tag="ACHJ1G_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(817..>1434)
                     /gene="iha"
                     /locus_tag="ACHJ1G_005583"
     CDS             complement(817..>1434)
                     /gene="iha"
                     /locus_tag="ACHJ1G_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4767200.1"
                     /translation="ANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHGVEFAGTLP
                     LWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYR
                     GKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAAVNNLLNK
                     DYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT          350 a          348 c          347 g          389 t
ORIGIN      
        1 aaaggcgaac ccgatacctg cctgttcatg cttaatgctc agggaaaggt cagtggcgat
       61 tctgacttta tcttttataa taatttgtct tctcctgagg gggcagtcag gctcgttacc
      121 ggttcgcagc aggccagcat tgagatagca ctggatcgtg ttccggcgaa cgtcagtaaa
      181 attgcgatca ccgttgtcat tgatggtgaa gataccatca gttcaggctg tacgcttact
      241 gacaaaatgg cgattgagaa ggtgcaattg acttatttgg atttggtcgt ggcgaggtgg
      301 tgaccctgca ttctgctaac cgggttatga gtcaggagtc agcgatagac aaagggactt
      361 atctgaccgg gcccctggaa aaatggcggg agagaatgat accttcctgc tgacgagacc
      421 ggactttgag atgacgagcg atagtgcggc agtgatgtac agcctgatcg gtagctgcaa
      481 aacttaacgg aatcgagccg gaaacgtggc tacgccacgt gatcagtgta atcaacacct
      541 ggcctgccaa ccgcatgaaa gagttgttgc cctggaacgt cactctctct gtaaactaat
      601 ttttacccca cgtccttcac ggtgcgctta cactctggac aagtatgtaa atgataagtt
      661 aaaaataatc cgtgcatttc ctgtgacgat attagttgtt ggtaacgaaa tctcattagc
      721 ggatcggaaa tgcacttaac ctaatacgtt gccactgttc cgccaggagt tgcagtggca
      781 acgtattcta ccgtcagtga tagcgttttg ttattatcag aactgatagt tcagcgacat
      841 ccagtaattt cgctcaggta tcacatatcc tgttgttgat gatcccgtct ggaagtaatc
      901 accggcatac agcgtactct taccggcact gtacaggctc acgtcactgt aatccttgtt
      961 gagcaggtta ttcaccgcag cattcagcgt cagggcatcc gtcatcttcc acgacagacc
     1021 tgcatccacc accgtccagg ctttcaggta ttctcctttc tcatcataca ctttcttctg
     1081 tacagcgctc agtgacgaat aattctgggt gaaacgtggt gttttcccgc gataacgggc
     1141 acccagccat gatgccacct cttcggtgat ctgccagttc agtttcgcat tcaccatgtg
     1201 ttcaggggta taactcagcg gcgcaccttt gttatcacca tcacgttgtt cacttcgggt
     1261 ccaggtgtaa ttcagtgaca gcgtgacatc ctctgaccac agcggcaatg tgccggcaaa
     1321 ttccacaccg tgcaaccggg cctttccgct gtttacatag ctattggtgt tatcatttat
     1381 ggaataagag acaatcttgt tggagaagtc agtcataaaa cctgtgacat tggc
//
LOCUS       DAZFDP010000415         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFDP010000415 DAZFDP010000000
VERSION     DAZFDP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..542)
                     /locus_tag="ACHJ1G_005584"
     CDS             complement(<1..542)
                     /locus_tag="ACHJ1G_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HFO4767201.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGV"
     gene            complement(562..1395)
                     /locus_tag="ACHJ1G_005585"
     CDS             complement(562..1395)
                     /locus_tag="ACHJ1G_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO4767202.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
BASE COUNT          473 a          317 c          246 g          392 t
ORIGIN      
        1 tctacccctt ctggggatat ttcaggtctg ccgttacaga aacggtctgg aaaaatttgg
       61 taccataccg tattttttac ccaggagggg gtatttagaa cgtcaatttt attaagataa
      121 gggaagcaaa aaaagttatt taagcggctt aactctcgtt cttcgcagtc cggtgaactg
      181 atatcaacac accttttctc accaaaaaga aacttctctc cctctttccc aaaaagtata
      241 aaaccgtaac ggcaacggcg tttttgaggt ttgaacactg caaaccagtg atcatgaaat
      301 tcggtgacag cttcttgttc catctcgatc tcgttgccgc caatccagcc gaacgcttct
      361 gtcccggcca tgttaccgcc gtcaagacca ccttcagccc aattatatgg atcgcctatc
      421 cacagaataa ctttatcaac ttctcctctt aatgtgcgta accgtacatg caaagtttcg
      481 ttatcatagg catagctatc ttcacttttt gctgagtgtt ttaacgaact aactgtaatc
      541 ataaatacta atccctaaaa attattcctt aacggcaccg ctaacaagtc cagtagttat
      601 atgcttctgt gtgaatacaa ataacaatgt gataggtact gcaactaaaa gcgccccagc
      661 agcaaaaagc gtataattct ctgctgaatt agaagcaatc caactaaaaa ttccaatcgc
      721 cagggtcatt ttatcctcac tacgaagcac caacgttggc aagatgaagt ccatccatgg
      781 tccagtgaac aaaactaatc caacgaaaac gagaatcggc ttagccaatg gcaatattat
      841 ctgaataaaa atagtcagat gccctgctcc atctatttta gcggcctcat ccaaagaagt
      901 tggaatagca tcaaaataac cttttaccaa ccacgtcatg aagggcaatg aaccggttac
      961 ataaaccaga agcaacccag tatatgtgtc tatcaaattg gcctttgata gcaggatata
     1021 aattgcagtc attgataaaa atgcaggaaa catttgcaat acaagaatac tcattaaagt
     1081 attacgcttt ccacgaaatc gataccttga aaaaacgtag gcggtcatcg tcacaacgaa
     1141 cagtgaaatt aacatgttag cggtagcaag aatgaaagtg ttcaaatacc actgcatata
     1201 tggcgtatcc gtgaacaacg ttatgtagtg gtcaaatgta aactcgatgc cactaaatgt
     1261 tgaactaaat agattattcc cttttttaaa agatgccatt atcgtccata tcactggccc
     1321 aagcacaaga atggagttgc agattaaaaa aagataaact aagagcgttg atattttacg
     1381 taaaaattta tccattgtca catccctaca tcatctttaa atgatttc
//
LOCUS       DAZFDP010000416         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFDP010000416 DAZFDP010000000
VERSION     DAZFDP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..340)
                     /locus_tag="ACHJ1G_005586"
     CDS             complement(38..340)
                     /locus_tag="ACHJ1G_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767203.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            438..809
                     /locus_tag="ACHJ1G_005587"
     CDS             438..809
                     /locus_tag="ACHJ1G_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767204.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            830..1021
                     /locus_tag="ACHJ1G_005588"
     CDS             830..1021
                     /locus_tag="ACHJ1G_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO4767205.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            1023..1301
                     /locus_tag="ACHJ1G_005589"
     CDS             1023..1301
                     /locus_tag="ACHJ1G_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4767206.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          433 a          315 c          307 g          364 t
ORIGIN      
        1 acattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaact ccatcagata
       61 agcatgcact acaatcacct tcagcatgaa ctacatgtgt ttgccccaaa cgaatgccgc
      121 tcgcatactc aggccaaata agctcccaat catgaggtcg tagctccgcc ctacttactt
      181 ggccttccgt cgcagattcg atcataaggg cgcgggttgg agatatagct gttcgtccag
      241 acgccatttg cgataagtaa gatggcgata caccaagtct ggccgcgaat ttcttagcat
      301 caccaaccct taatgattta ataaactctt ttaatgtcat accttcctcg gtttagtgtt
      361 tttttgcaag tttagtgttt aataaaccat taagtcaagt atttgcttgt ttagtgatta
      421 ctaaagataa ttaccacatg caaaaaaaag aaattcgccg tttacgtctc aaggagtggt
      481 ttaaagataa aactctgcca cccaaagaga agagctacct atctcaacta atgagtggga
      541 gagcctcgtt tggagaaaag gctgccagaa gaatagagca aacatacggg atgccggaag
      601 ggtatctgga tgcggaatac gcagaacaac cgggggtttc tccaccacat gcagggttaa
      661 cgtctaatca actggaatta ttgcagattt tttcagcctt ccctgaggat gagcaacgcc
      721 agataatcag cgagttaaag cagaaaaaag aatcaatgga agatctcata gcgagatgga
      781 ttgcggcgca aaaatgccgc cgcgcctgag ttataaaacc ggaggaaaca tgaatagagc
      841 cctttcacca atggtttctg aatttgaaac cattgaacaa gaaaacagtt acaacgaatg
      901 gctgcgtgcg aaagtagcaa cgagccttgc agatccgcgc ccagcaattc cccatgacga
      961 agttgagcgc agaatggcag aacgctttgc taagatgcgc aaggaacgga gcaagcagta
     1021 aaatgttacc cgtgttatgg cttgaaagcg cagataccga cctagatgat ataactagtt
     1081 atattgctcg tttcgacata gatgcggctg aacgcttatg gcagcgatta aggggttgtg
     1141 tgctgccgtt atccgaacat ccgtatttat acccaccaag cgacagagta cctggcttgc
     1201 gtgagattgt agcccaccct aactatataa ttctataccg cgtaacaaca tcaagcgttg
     1261 aagtagtaaa cgtgatccac gcaagacgcc agtttcccta actttcacta ccaatagaaa
     1321 cataacaacc gcaacgactt tatcaaaagc gttgtgtttg ttatgtcccg cggtttagtt
     1381 tttacttgac ttaagtttaa tgtttattaa actaaaaat
//
LOCUS       DAZFDP010000417         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFDP010000417 DAZFDP010000000
VERSION     DAZFDP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1364)
                     /locus_tag="ACHJ1G_005590"
     CDS             complement(<1..1364)
                     /locus_tag="ACHJ1G_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4767207.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVA"
BASE COUNT          297 a          453 c          365 g          293 t
ORIGIN      
        1 gccaccaccg cttccgccag cgcattacgg accagtgcat cagcagccgg actggaaaaa
       61 cggatcaatt cttccgtcag taccgcaatg gccgacactt tcgcatgact gaaggtgatg
      121 gattcaaaat caaacttcgt caggggtctg gccttaccct cacccaccca gccggcagca
      181 ccaccggaca cctgggcatg cacgcggata ttgaacggca cctgacgaag tgcagggatc
      241 ccgccctgac caaatcgccc gataatggtc tgcggacgca ggtaatcaat aaagtcctgc
      301 gcatattcct ggtattcaga caggctgcct gcccactgcg ggtccgtggt ggtccctgcc
      361 cccaccgccg atttcaggac atgatgcaga cggctgtcat ccggatactg acgacgggcc
      421 acttccaggg cttcagagcg gacaccttta gccgcggcca gtgatttggc aaagcgggcg
      481 aaaccaatcc ccttctccag tttctgctca acacggatca ccggcgctga agccaccgtg
      541 gccacattcc cgttaccggc ctgtttcacc ggctgtgccg tggcggcctt actggtttcc
      601 agttcacgca gacgcttcag gtgcgcatcc acctgacgga tttccgctgc ggtgttgtcg
      661 tagtgctctt cctcttccac atccagtgtg cggccttcct ctgcggcttt gttcatgatc
      721 tcctcaaggg aggctgccag cgccgcacgc ttgttttcaa aacttttaat ctgttcacca
      781 gtattcattg ctgacttttc cttatgaaaa gaggttattg actgtgccga agcgccggca
      841 gaagatgcga ttttcaccac cggtttccgg ttgccggacg cggcagaaaa cgggcggtcg
      901 aaatatttaa tggtccggat ggtgcattcc gcattcgcgg gcacggtgac ggcagacacc
      961 tccatcagct cccagcgcag aaaatgcagt ccgcctccgt ccagataagt gtattcatgg
     1021 ggccggaagc ccacagaaag ccccctgacc agcccggtct taatggcagc ccaggcctca
     1081 tccagccggg ctgccatctg ggagggcatc cccggctccg gcttcaccag cattgccgtg
     1141 atttccagcc cttccctgac ccgacgcacc gtacactggc ctacagggcg ggaatggtca
     1201 tgctgccaga gaaacgggat cgtactgcca aactccgccc cctccggctc caggatgtca
     1261 ccatcccgat ccggagaagg cgttgacgca atcccggtga tcacccgttc atcctcactg
     1321 aaggatttca ccgtcagcag ggaacaggcc cgtttaagag tcacatcagc ctcctgaaaa
     1381 taaaaaaacc gccgcagcgg ttcatgat
//
LOCUS       DAZFDP010000418         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFDP010000418 DAZFDP010000000
VERSION     DAZFDP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..486)
                     /locus_tag="ACHJ1G_005591"
     CDS             complement(<1..486)
                     /locus_tag="ACHJ1G_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4767208.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQKEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AE"
     gene            complement(588..1388)
                     /gene="gpM"
                     /locus_tag="ACHJ1G_005592"
     CDS             complement(588..1388)
                     /gene="gpM"
                     /locus_tag="ACHJ1G_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO4767209.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
BASE COUNT          241 a          434 c          411 g          318 t
ORIGIN      
        1 ctccgcaaag attcgcgggc ggcacagaat gtcagagatg gcccagctca catcgcgcca
       61 caaatccgat gtctgtatat ccagtgcgtc cgcccggcgg tcgcccttgt ccgttgtgtc
      121 cgcatcgcgg taacgctcca gaatcagggc gcgtgtggcg gtgtaaaccg cattgcgcca
      181 gtgccagaga ttgacgcttt ctccgttaat tacgggtgcc ggaacatcgg ccagcgtctg
      241 atggccagcc gcctgctgtt ccttctgcca tgcttccagc tcgcgggtaa cgtgtgccac
      301 agctccggtg gcggtatgca gcaggcggga ggtggtcacg cgccccggca gtcgtaccgc
      361 cagacgcagt tcacgcagca caatatccgg ccagaatgca cccgctgaaa tacgggtatc
      421 accatcatcg gtatcggtga tgtcgtcctc tgcgggtccg gggttggttc tggcaaccat
      481 actcatgggg ttcactcctg aaaaaatcgg gcggtgggtg cgcggtgtaa acggtcacgg
      541 agtcaaaccg gaacaccgcg cacgccgccc gctgacgggg tcagtcgtta accgcgcttc
      601 gccttctgcg tcgcggtggt ttttcgtgtt gcaggcttcc gcgttgtctt tttacttttg
      661 ctgctttcgt cctgcgcctg ctgtgcgctg gcgtcttctg gtgcggctgc ggaatcggct
      721 tttttcaggg cgcgggaaag ggttgcaatc tcgcgtttca cacctgcgtt cgggttcagg
      781 tgcattgctt cgcgcagcag cttcagtgac agggccatgc tgtccgcatc actcaggcca
      841 cggcgggcaa aggcgcacgc tttgcataat ttggcgcgca cttcgtccgg catgtcctgg
      901 tcggtgacaa tctcccggag ggtgtccagt ggttcgataa aggcggacaa atccgcgtcg
      961 gcatccgtcc cggcctgcgt cagtaccgga ttacagattt cttcggtcag taccgtggca
     1021 gcagtacggc caaagttatc cggcatgatg aggttgtgac ggaccacata cgcgccgata
     1081 cgcagcgcaa gcggaagatc gccacagtca atcgcccaca ccatcagcgt ggcaatcact
     1141 tcatcctgct gcccgccgtc agcctccagc gttccctcaa tccagccgga aaaatccggc
     1201 aacaactctt ttttgatggc ggctttcgcg cttctggcct gtacaccctt aagccgggcc
     1261 tgtgccagac gcagacgata cagcacctct tcatgcgcgg tacgcgcggc gtggtccacg
     1321 ccttcattcg cccggcctgc gcgctgtgcc atcacgttct gccagtgttg ctgtgcagga
     1381 gtaatcattt gttctctccg ttac
//
LOCUS       DAZFDP010000419         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFDP010000419 DAZFDP010000000
VERSION     DAZFDP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..486)
                     /locus_tag="ACHJ1G_005593"
     CDS             complement(<1..486)
                     /locus_tag="ACHJ1G_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4767210.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAI
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILRRPRIF
                     AE"
     gene            complement(588..1388)
                     /gene="gpM"
                     /locus_tag="ACHJ1G_005594"
     CDS             complement(588..1388)
                     /gene="gpM"
                     /locus_tag="ACHJ1G_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO4767211.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSTFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQTQDESSKSKKTTRKPATRKTTATQKAKRG"
BASE COUNT          244 a          431 c          411 g          318 t
ORIGIN      
        1 ctccgcaaag attcgcgggc ggcgcagaat gtcagagatg gcccagctca catcgcgcca
       61 caaatccgat gtctgtatat ccagtgcgtc cgcccggcgg tcgcccttat ccgttgtgtc
      121 cgcatcgcgg taacgctcca gaatcagggc gcgtgtggcg gtataaacag cattgcgcca
      181 gtgccagaga ttgacgcttt ctccgttaat tacgggggcc ggaacatcgg ccagcgtctg
      241 atggccagcc gcctgctgtt cctgctgcca cgcttccagc tcgcgggtaa cgtgtgccac
      301 ggccccggtg gcggtatgca gcaggcggga ggtggtcacg cggcccggca gtcgtatcgc
      361 cagacgcagc tcacgcagca caatatccgg ccagaatgca cccgctgaaa tacgggtatc
      421 accatcatcg gtatcggtga tgtcgtcctc tgcgggtccg gggttggttc tggcaaccat
      481 actcatgggg ttcactcctg aaaaaatcgg gcggtgggtg cgcggtgtaa acggtcacgg
      541 agtcaaaccg gaacaccgcg cacgccgccc gctgacgggg tcagtcgtta accgcgcttc
      601 gccttctgcg tcgcggtggt ttttcgtgtt gcaggcttcc gcgttgtctt tttacttttg
      661 ctgctttcgt cctgcgtctg cggtgtgctg gcatcttctg gtgcggctgc ggaatcggct
      721 tttttcaggg cgcgggaaag ggttgcaatc tcgcgtttca cacctgcgtt cgggttcagg
      781 tgcatcgctt cgcgcagcag cttcagtgat gaggccatgc tgtccgcatc gctcaggcca
      841 cggcgggcaa aggcgcacgc cttgcataat ttggcgcgca cttcgtccgg catatcctgg
      901 ttggcgacaa tttcccaaag tgtgtccagt ggttcgataa aggtggacaa atccgcgtcg
      961 gcatccgtcc cggcctgcgt caataccggg ttacagattt cttcggtcag taccgtggca
     1021 gcagtacggc caaagttatc cggcatgatg aggttgtgac ggaccacata cgcaccaata
     1081 cgcagcgcaa gcggaagatc gccgcagtca atcgcccaca ccatcagcgt ggcaatcact
     1141 tcatcctgct gcccgccgtc agcctccagc gttccctcaa tccagccgga aaagtccggc
     1201 aacaactctt ttttgatggc ggctttcgcg cttctggcct gtacgccctt aagccgggcc
     1261 tgtgccagac gcagacgata cagcacctct tcatgcgcgg tacgcgcggc gtggtccacg
     1321 ccttcattcg cccggcctgc gcgctgtgcc atcacgttct gccagtgttg ctgtgcagga
     1381 gtaatcattt tttctctccg ttac
//
LOCUS       DAZFDP010000420         1400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFDP010000420 DAZFDP010000000
VERSION     DAZFDP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..583
                     /locus_tag="ACHJ1G_005595"
     CDS             <1..583
                     /locus_tag="ACHJ1G_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767212.1"
                     /translation="PSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLKPDPSDKRSSCP
                     DASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASYD
                     GEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREK
                     WDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            624..1046
                     /locus_tag="ACHJ1G_005596"
     CDS             624..1046
                     /locus_tag="ACHJ1G_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4767213.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            1043..1297
                     /locus_tag="ACHJ1G_005597"
     CDS             1043..1297
                     /locus_tag="ACHJ1G_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767214.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            1290..>1400
                     /locus_tag="ACHJ1G_005598"
     CDS             1290..>1400
                     /locus_tag="ACHJ1G_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767215.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEF"
BASE COUNT          391 a          285 c          394 g          330 t
ORIGIN      
        1 cccctcaacg gttgacccgt caaattttga tggatcaact gttgataaaa aactgccgat
       61 tagggggccg atgattgacc ccgatccgtc agtattaaaa cctgatccgt cagataaaag
      121 atcttcttgt ccggacgctt cgcaaccgga cccgcagacg gctgaacagg attttttaac
      181 ccgacaccct gacgcggttg tgttcagtgc gaaaaaacgc cagtggggaa gtcaggaaga
      241 tttggtgtgc gcacagtgga tctggggacg aatcgtgagt ctttacgagc aggcggccag
      301 ctatgatggc gagatcacta gaccgaaaga acccaactgg acagcatggg ccaatgacgt
      361 tcgcacaatg cggatgctgg atggcagaac tcacagacaa atttgtgaaa tgtttgggcg
      421 tctccagcgg gattcgttct gggtaaaaaa catcatgagt ccggcaaaac tccgggaaaa
      481 atgggatgaa ctggttatcc gcctggggcg ttcgcctgcg cagcgttgcg tgaatcacat
      541 ttctgaaccg gacactgaaa ttccgccggg cttcaggggg taagtgttaa tttctggtca
      601 tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac acaagaagag cgggaaaaaa
      661 ttaaagggca ggttgttgaa ctcgtacgcc agagtgggcg cgagacgtta cgacaactgg
      721 aaactaaaac tggggcaaca agatatctga tgagcgttct ggccagagag ctggttgcca
      781 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      841 ggcaaaatgc ccgtaaaaag ctctcaaggg caaagccgaa gaaaccatct gcggttgatc
      901 cggaccttat ctggtcatta ccagatggcg aaatacgccg ctacgacagg cgtctgaaca
      961 taatctgtct cgagtgccgg aagagcgaag ttatgcagcg cgtactggcg ttttatcagg
     1021 ggaattttga ggaggtggtg cggtgagtga atcaaaatgc caggttaatg gcaacaagat
     1081 agaaccatgt gcagcactgg caaagtccct tgagcatgat gctgaataca cgatgcgaaa
     1141 aggtctgctg atatacaaaa tctggaatga gagtttaact cgcggtcctg attttgtgat
     1201 gttgcgttcc ggtgaatttt ctaaattacc agttcgggtt tcattttgtc cgttctgtgg
     1261 tgaaagtctg aaaacgtggg agaacagaaa tgaatgaaat taaagaaata ccagtagtac
     1321 gtgatgaata tggctgctgg acgcatcctg aatatgaaaa attctgtgac ggtcgggaat
     1381 atatttcaac ggaagagttt
//
LOCUS       DAZFDP010000421         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFDP010000421 DAZFDP010000000
VERSION     DAZFDP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..697
                     /locus_tag="ACHJ1G_005599"
     CDS             <1..697
                     /locus_tag="ACHJ1G_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4767216.1"
                     /translation="PAESCGFVVRTPEGERYIPCVNISAEPEAYFRIAPEDWLRAEMQ
                     GEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFRCVPHLTGRLFEHGV
                     TDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQPGDIL
                     LCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHVSAFT
                     GIYNDLAAASACM"
     gene            694..1275
                     /locus_tag="ACHJ1G_005600"
     CDS             694..1275
                     /locus_tag="ACHJ1G_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4767217.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRAMDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQR"
BASE COUNT          298 a          347 c          454 g          298 t
ORIGIN      
        1 gcctgcggag tcgtgtggct tcgtggtgag aacgccggag ggggagcggt atatcccttg
       61 tgtgaatatc tctgcagagc cggaggcgta ttttcgtatt gcaccggaag actggctgcg
      121 ggcagagatg cagggggaga ttgtggcact ggtccacagt catcccggtg gtctgccctg
      181 gctgagcgag gccgaccggc ggctgcagat aaaaagtgca ctgtcctggt ggctggtctg
      241 ccggggggac attcataaat tccgctgtgt gccacatctg acaggacggc tctttgagca
      301 cggggtgacg gactgttaca ccctgttccg ggatgcatac catctggcgg gaattgatat
      361 gccggatttt gagcgtgagg atgactggtg gcgcaatggt cagaacctgt acctggacaa
      421 tatggaggcg actggttttt acaggatttc cctgccttcc gcacagcctg gcgatatcct
      481 gctgtgctgc tttggcgcat cggtggccaa tcatgccgcc atatactgcg gcaacggtga
      541 actgcttcac catctgcctg aacaactgag taaacgggag aggtattctg aaaaatggca
      601 acgacgaacg cattctgtct ggcgtcaccg ccactggcac gtatctgcct tcacggggat
      661 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccatccgg
      721 gcgctgtcgt tacaggtgcc gggattccgc cgtcagatga acgaaggctg gtaccagata
      781 cgtattcgcg gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacctctg
      841 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
      901 cagattgtgc tgggggcggc ggccatcgtg gggtcgttct tcacggccgg ggcatcaatg
      961 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
     1021 ctgggggcca gcatgatact gggcggtgtg gcccagatgc tggcaccgaa ggcaaaaaca
     1081 ccggattacc gcgcaatgga taacggcaga cagaacacgt acttttcctc actggacaac
     1141 atgattgctc aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggttcacga
     1201 cggatatccc aggacatcag cacccgtgat gagggcgggg gcggaaaggt cgtggttatc
     1261 gggcggcaga ggtaaaaaga ataaaaaaat cccgcagtgt atggaggctg cgggaacaga
     1321 aaatgaagat taaccacagg gagttttgtt tttattggcc cgaaaaaact gtaacgcccg
     1381 ggaatgatat ctgccac
//
LOCUS       DAZFDP010000422         1370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFDP010000422 DAZFDP010000000
VERSION     DAZFDP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1370
                     /locus_tag="ACHJ1G_005601"
     CDS             <1..>1370
                     /locus_tag="ACHJ1G_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4767218.1"
                     /translation="IVDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFIL
                     IPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDD
                     LCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLRE
                     AIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPE
                     MVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGL
                     ALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETRE
                     WLGWGHAWVHETAVVRRKSEVSRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAEL
                     LDHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLVHGGQ
                     PLMAWCV"
BASE COUNT          276 a          330 c          474 g          290 t
ORIGIN      
        1 attgtggatg caccgggtag cccgacattc ggggagtcct gtgcaccgtg ggtgtttgac
       61 ctggtggcgg ccctgtttgg ctcctacgat gcgcagaccg gtgttcgcca tatcaaggaa
      121 gtgtttatcc ttatccccaa gaaaaacagt aagtccacgc tggccgcggg gatcatgatg
      181 acggcgctgt tactgaactg gcggcaggcg gcgggctaca ccattctggc cccgaccgtg
      241 gaggtggcgg ctaacgcctt caaccctgcc agggatatgg tacgacggga cgatgatctg
      301 gatgacctct gtcaggtgca gacacatatc cggaccatca cccatcgggt gacggacacc
      361 accctgaagg tggtggcagc cgatccgaat acggtgtccg gtatcaagtc cgtggggaca
      421 ctgattgatg aactgtggct gtttggcaag cagtacaagg cggaagacat gctacgtgaa
      481 gccatcggcg ggctggcttc acgtccggaa gggtttgtgg tgtacacaac cacccagtcg
      541 aatgaaccgc ctgccggggt gttcaggcag aaactgcagt acgcccggga tgtccgtgac
      601 ggcaaaattc atgatccgca ctttctgccg gtgatttttg agcatcctcc tgaaatggtg
      661 gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg ttaacccgaa tctcggttat
      721 tcggtggatg aggcctttct gtaccgggag tatcgtaaag cccgggaagc cggggaagag
      781 actttccgcg gtttcatgtc aaaacacgcc aatgtggaaa ttggtcttgc cctgcgttct
      841 gaccgctggg caggggcgga tttctgggag cagcagggca ggcgcgtcag cctggacgat
      901 atcctgcagc gcgctgatgt ggtgacggtg gggattgacg gcgggggcct ggatgatctg
      961 ctgggaatgt acgtgattgg ccgtgacagg gaaacccgcg aatggctggg ctggggccat
     1021 gcctgggtgc atgaaaccgc ggtggtcaga cggaagagtg aggtatcccg gtttcaggat
     1081 tttgtggcct gtggagacat gacgattgtc cgtcgggtcg gggatgacac ggcggaagtg
     1141 gcggagtatg tgcgtcgtat tcatgaggct gagttactgg atcatatcgg tattgacccg
     1201 tcaggtgtgg ggcagattct ggattcactg gcggaagccg ggatccccga tgagagtgtg
     1261 gtggggataa gccagggctg gaagctgggc ggggccatca aaaccaccga gcgcaaactg
     1321 gctgagggag tgctggtgca tggtggtcag ccactgatgg cctggtgcgt
//
LOCUS       DAZFDP010000423         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFDP010000423 DAZFDP010000000
VERSION     DAZFDP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..900
                     /locus_tag="ACHJ1G_005602"
     CDS             61..900
                     /locus_tag="ACHJ1G_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO4767219.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
     gene            923..>1369
                     /locus_tag="ACHJ1G_005603"
     CDS             923..>1369
                     /locus_tag="ACHJ1G_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO4767220.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVAS"
BASE COUNT          380 a          228 c          303 g          458 t
ORIGIN      
        1 atgtggtagg ggagtgctta gatgtaatat ttcttttgtt tattgaggtt aatttaaatt
       61 atgaacgata ttgagaacta tgggaatcga tatcatgtgt tgcaatcctt tccgatgcca
      121 caattggcaa gtagtcgcga aataagaata ttactacctg cgtcctatta tgaatctgat
      181 gtcagttacc ctgtattgta tatgcacgat gggcaaaatt tatttgataa atctgtatct
      241 tttggtccac atgtatggga tatacctgag gcggtagatg cttttttccc aaattcccaa
      301 tacgacggtg ttatcattgt ggggattgat aatgcatcgt ctcatggtaa attcagccgt
      361 atggatgagt attctccatg gccgctaaac acatcttttc ccttacctgg ctgggaccct
      421 gatgtggatt atagtggagg aaaaggtgcg ttgtatgttg attttattgt gaatacgctc
      481 aagaactata ttgacagcaa ttttcgaact ctcccagatc gtaataatac agcgatagca
      541 ggtagttcta tgggtgcgta catcagtttg tttgccgcaa ttcttcgtct agacgttttt
      601 tcaaaagttg gcgttttttc gccggcacta tggttcaatg acagcgcaat gctgaatttt
      661 attcaggaaa acaatatctt tgcagatctt actgtatatc tcgatgtcgg tacgctggaa
      721 accagtggta tgcgcgaaga ctttcctgaa gtctatattt caggcgcgga aaaattatgc
      781 gtgagtctcc gtaaacaacg caatgtaact atagattatc atctatgggg aggtgatacc
      841 cactctgagt ctgcatgggc aaaacgtttt ccagaaatgt tgaaattatt ttattgctga
      901 tgaaaccggt gaaggatgtt gaatgcatca tattaaaaca gccgcagatc ttaattgttt
      961 tttaaattca atggttgccg tttcgcaacc aactgttgat aaaattattt ttggtgctga
     1021 ggcgacactt attaataaaa ttgggacctg ctggattgcc tcaattgatg ttttaagaaa
     1081 ggctgttttt gaaggtgcca atatcattat tactcatgag cctacatttt attcttatgc
     1141 tgatctggag ggtgacgttc ttgagttttc atgggccaga aaaataatgg attatacgcg
     1201 cggtgaactc agttatttaa aaataattga gcaaaaaaag gagtttttgc ataaaaataa
     1261 tttggtaatc attcgttgcc atgatgtcat ggatcgagag cctacgttcg gaatgtctaa
     1321 ggctttggct cagcagttag aactggatgt tacaaatatt gttgcttct
//
LOCUS       DAZFDP010000424         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFDP010000424 DAZFDP010000000
VERSION     DAZFDP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..435
                     /locus_tag="ACHJ1G_005604"
     CDS             <1..435
                     /locus_tag="ACHJ1G_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4767221.1"
                     /translation="RCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWR
                     NGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQL
                     SKRERYSEKWQRRTHSVWRHRHWHASAFTGICNDLAAASACM"
     gene            432..1013
                     /locus_tag="ACHJ1G_005605"
     CDS             432..1013
                     /locus_tag="ACHJ1G_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4767222.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGYDMAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          311 a          336 c          435 g          283 t
ORIGIN      
        1 cgctgtgtgc cacatctgac aggacggcgc tttgagcacg gggtgacgga ctgttacacg
       61 ctgttccggg atgcatacca tctggcggga attgatatgc cggattttga gcgtgaggat
      121 gactggtggc gcaacggtca gaacctgtat ctggacaata tggcggtcac cggcttttac
      181 cgggtgcccc tgtcctctgc acaggcgggc gatatcctgc tgtgctgctt tggcgcatcg
      241 gtggccaatc atgccgccat ttactgcggc aacggtgaac tgcttcacca tctgcctgaa
      301 caactgagta aacgggagag gtattccgaa aaatggcaac gacgaacgca ttctgtctgg
      361 cgtcaccgcc actggcacgc atctgccttc acggggattt gcaacgattt ggccgccgcc
      421 tcagcctgta tgtgaacacg gcagcggaag ccatccgtgc cctgtcgatg cagatgccgg
      481 gattccgccg tcagatgaac gaaggctggt accagatacg tattgccggt tatgacatgg
      541 caccggaggc ggtgtatgcc cgccttcacg aacaactggg tgagggaacg gtcatccata
      601 ttgtgccgcg actggccggt gcagggaaag gcggactgca gattgtgctg ggggcggcag
      661 ccatcgtggg ctctttcttc accgccggcg caacgatggc gttatggggt tcagccctgg
      721 cagccggcgg tttttctgcc accacgatgc tgttttcact gggggccagc atgatactgg
      781 gcggtgtggc ccagatgctg gccccgaagg caaagacgcc ggagtacaaa agtacggata
      841 acggtaaaca gaacacgtat ttttcgtcac tggacaacat gattgcccag gggaacccga
      901 tgccggtgcc ttacggtgaa atgctggttg gctcccggcg aatctcccag gacatcagca
      961 cccgtgatga aggtggtggc gggaaggtgg tggttatcgg gcggcagggg taaagcataa
     1021 aaaaatcccg cagtgtatgg aggctgcggg aacagaaaat gaagattaac cacagggggt
     1081 tttgttttta ttggcccgaa aaaactgtaa cgcccgggaa tgatatctgc cacggggcgt
     1141 acagaaaatg tgaagaaatt cagaaatttt attccgtcat gacacaggca ccctccgggg
     1201 tgcctgtcgt ttttggggca taaacagatt cagacatcag acaggagagg gggacagagt
     1261 gggtaaaggt ggcggcaggg cgcacacgcc ggtagaggca aaggacaatc ttaagtccac
     1321 gcagatgatg agcgtgattg acgccattgg tgaagggccg attga
//
LOCUS       DAZFDP010000425         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFDP010000425 DAZFDP010000000
VERSION     DAZFDP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..566)
                     /gene="sfmF"
                     /locus_tag="ACHJ1G_005606"
     CDS             complement(51..566)
                     /gene="sfmF"
                     /locus_tag="ACHJ1G_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4767223.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(577..>1326)
                     /gene="fimH"
                     /locus_tag="ACHJ1G_005607"
     CDS             complement(577..>1326)
                     /gene="fimH"
                     /locus_tag="ACHJ1G_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4767224.1"
                     /translation="IVQETIDNYKYMQLHDYLLGAMSLVDSVMDIQFPPQNYIRMGTD
                     PNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTIS
                     FGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVA
                     AQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAAN
                     VTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          377 a          341 c          283 g          325 t
ORIGIN      
        1 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
       61 ttgatcataa atatcgcatc cgctttcgcc acaccaggcc gaacaccgct ggctaacgct
      121 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
      181 ctcgcctccc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt
      241 tgtgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
      301 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
      361 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
      421 ttatcaatat ccgctgtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
      481 atattaatcg ttcctaaagg gtcagctgcc catccaatgg aactccacag taacccgcag
      541 aaacagctaa agagtactct tctcattctc cgtacctcat tgataatcga cgcgtaaata
      601 ccccagcgcg ctaaacggcc cctcggtcgg tttttgaccg gtaatactga taggccaggc
      661 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
      721 atcgttaggc gtaatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
      781 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
      841 ctgcgcagca acattcgtac atttgaccgc aatgctcttg gtttgcggca tgacgccagc
      901 tggtcgattc cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
      961 ttcaacaatc tgcccggcat ttaattcgca gttttgcggc acttcaaccc ggccaccaaa
     1021 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     1081 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     1141 aaatattaaa cgagaatcca tcaccccgaa tggaaggttt tgcgaaacgt taggatctgt
     1201 tcccatccga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     1261 catcgcacct aatagataat catgtaattg catatattta taattatcga tagtttcctg
     1321 aacaat
//
LOCUS       DAZFDP010000426         1317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFDP010000426 DAZFDP010000000
VERSION     DAZFDP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..453
                     /gene="ydeI"
                     /locus_tag="ACHJ1G_005608"
     CDS             61..453
                     /gene="ydeI"
                     /locus_tag="ACHJ1G_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4767225.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            708..>1317
                     /gene="dgcZ"
                     /locus_tag="ACHJ1G_005609"
     CDS             708..>1317
                     /gene="dgcZ"
                     /locus_tag="ACHJ1G_005609"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4767226.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          369 a          257 c          307 g          384 t
ORIGIN      
        1 caattataaa aatagcaacc agacttaata agtcaaacca aacgcaatta aggagttagt
       61 atgaaatttc aggccattgt attagcaagt tttcttgtca tgccttatgc gttggcagat
      121 gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc ctcatgcaat agaagatggc
      181 tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt tcgcaaaaaa catgcacgat
      241 ggcgcttcgg tttcactacg cggtaatttg atttctcaca aaggagagga ccgttacgtt
      301 tttcgcgata agagcggtga aattaatgtc gttattcctg cggccgtctt tgatggacga
      361 gaagttcagc cggaccagat gatcaacatt agcggcagtc tggataagaa aagcgcgccg
      421 gccgtcgttc gggtcactca tttgcagaaa taaaaataaa ttagcctgat ggcctgaact
      481 actgggccat tagggtattg ggttatgcta tttgtcatcc ttttatttac gataatgcag
      541 aggtgattta cagaattaaa cactttttta tccgctgatt ttgttttaaa tcacaatgtt
      601 atctcagaga tgactcataa cttcggattt ctatgaaaat attgacgtaa ttttgctttt
      661 ctctcgttag aatagcgcgc acaaggaact gtgaaaaagg agtggcaatg atcaagaaaa
      721 caacggaaat tgatgccatc ttgttaaatc tcaataaggc tatcgatgcc cactaccagt
      781 ggctggtgag tatgtttcac agcgtggtcg cgagagatgc cagtaagcca gaaataacgg
      841 ataaccattc ttatggactg tgccagtttg gtcggtggat tgatcatctg gggccactcg
      901 ataacgatga attaccttac gttcggctaa tggattctgc ccatcaacat atgcataact
      961 gtggtcggga attaatgctg gctattgttg aaaatcactg gcaggacgcg catttcgact
     1021 cctttcagga ggggttgctt tcttttactg cggcattaac cgattacaaa atttatttgc
     1081 tgacgatccg tagcaatatg gatgttttga cgggattgcc ggggcgtcgg gttcttgatg
     1141 aatcctttga tcatcagtta cgcaacactg agcctctgaa tctttattta atgttgttgg
     1201 atattgaccg atttaaattg gttaatgata cctacgggca tttaatcggc gatgtagtat
     1261 tacgcaccct ggcaacttac ttagccagtt ggacgcgtga ttacgaaacg gtttatc
//
LOCUS       DAZFDP010000427         1317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFDP010000427 DAZFDP010000000
VERSION     DAZFDP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..911
                     /gene="espB"
                     /locus_tag="ACHJ1G_005610"
     CDS             <1..911
                     /gene="espB"
                     /locus_tag="ACHJ1G_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO4767227.1"
                     /translation="VNSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQKLLGK
                     MVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGILGSFA
                     AINSATKGASDIAQKTVSTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQQAMT
                     TATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGSTPFIA
                     VTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDDIASR
                     LRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            918..>1317
                     /gene="cesD2"
                     /locus_tag="ACHJ1G_005611"
     CDS             918..>1317
                     /gene="cesD2"
                     /locus_tag="ACHJ1G_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO4767228.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNV"
BASE COUNT          369 a          274 c          321 g          353 t
ORIGIN      
        1 cggttaattc tgtttcggag aatactaccg gctctaatgc aattaccgca tctgctatta
       61 attcatcttt gcttaccgat ggtaaggtcg atgtttctaa actgatgctg gaaattcaaa
      121 aactcctggg caagatggtg cgtatattgc aggattacca acagcaacag ttgtcgcaga
      181 gctatcagat ccaactggcc gtttttgaga gccagaataa agccattgat gaaaaaaagg
      241 ccgctgcaac agccgctctg gttggtgggg ctatttcatc agtattgggg atcttaggct
      301 cttttgcagc aattaacagt gctacgaaag gcgcgagtga tattgctcaa aaaaccgtct
      361 ctacatcttc taaggctatt gatgcggctt ctgatactgc gactaaaacg ttgactaagg
      421 caacggaaag cgttgctgat gctgttgaag atgcatccag cgtgatgcag caagcgatga
      481 ctacagcaac gagagcggcc agccgtacat ccgacgttgc tgatgacatt gccgattctg
      541 ctcagagagc ttctcagctg gctgaaaacg ctgcagatgc cgctcagaag gcaagtcggg
      601 caagccgctt tatggctgca gtagataaga ttactggctc tacaccattt attgccgtta
      661 ccagtcttgc cgaaggcacg aagacattgc caacaacggt atctgaatca gtcaaatcta
      721 accatgagat tagcgaacag cgttataagt ctgtggagaa cttccagcag ggtaatttgg
      781 atctgtataa gcaagaagtt cgcagagcgc aggatgatat cgctagccgt ctgcgtgata
      841 tgacaacagc cgctcgcgat ctcactgatc ttcagaatcg tatgggtcaa tcggttcgct
      901 tagctgggta attgatcatg gtcgatacgt ttaatgatga agtgcttaat cactatcttg
      961 aacaaaaagg gtacacaata cagaaggagt ttctttgtgg cagtgccttt tttatcggat
     1021 ggcggattga gacctctttt ttctcattag cgtacagact tgatgaacaa gaactgattt
     1081 tgtgctcttt tgaagcacgt aaccaaacag ggcttaacgg ccctgtttta tcactgactc
     1141 gcttgctcga agagttgtac caccattttt cgggtattaa gaaaatcagt gcgatgaaat
     1201 ctaagattgg ttcagattca gaacgtcaaa agcgcgaaga gttgtttaat tacttcatca
     1261 gaaagggcgc tgtgcagcaa gaaacagaag acggaatttg gttcgtaatg aatgtaa
//
LOCUS       DAZFDP010000428         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFDP010000428 DAZFDP010000000
VERSION     DAZFDP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..905
                     /locus_tag="ACHJ1G_005612"
     CDS             54..905
                     /locus_tag="ACHJ1G_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HFO4767229.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DTPESAQ"
     gene            complement(938..>1309)
                     /locus_tag="ACHJ1G_005613"
     CDS             complement(938..>1309)
                     /locus_tag="ACHJ1G_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4767230.1"
                     /translation="LLEQKAHQLNQQLVAAAPKVDFADRVSVAKGILIGNFAKVVGLK
                     QNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTGK
                     GQQWLTRKLLDAGLLKPVAAE"
BASE COUNT          377 a          335 c          291 g          306 t
ORIGIN      
        1 accggttttc tcagccgggc gttattgacc atgtcaattc tggaggagga tcaatgataa
       61 attatgtcta cggcgaacaa ctgtaccagg agttcgtcag cttcagggat ctctttctaa
      121 aaaaagctgt tgcacgcgcc caacacgttg atacagccag cgacggtcgt cctgtacgcc
      181 cggttgtcgt tctaccgttc aaagaaactg acagcattca ggctgaaatt gataaatgga
      241 ctttaatggc gcgggaactg gaacagtacc cagacctcaa tatcccaaag actattttat
      301 atccagtgcc taacatcctt cgcggtgtgc gtaaggttac aacttatcag acagaagctg
      361 tgaacagcgt caacatgacc gctggccgca ttattcatct gattgataag gacattcgca
      421 tccaaaaaag cgcggggatc aatgagcaca gtgcgaaata catagagaac ctggaagcaa
      481 caaaagagct aatgaagcag tacccggagg atgaaaaatt ccgtatgcgc gtacacggct
      541 ttagcgaaac aatgctgcgc gtccactaca tttccagtag ccctaactac aatgatggta
      601 aatcagttag ttaccatgtg ccactgtgtg gcgtgtttat ctgcgatgaa actctccgtg
      661 atggaattat catcaacggt gaattcgaaa aagcaaaatt tagcctttat gactccatag
      721 aaccgatcat ctgcgaccgc tggccgcaag caaaaatata tcgcctggca gatattgaaa
      781 atgtaaaaaa acaaattgcc atcactcgcg aagagaaaaa ggttaagtca gccgcatcag
      841 ttacgcgcag ccgtaaaacc aagaaggggc agccagtaaa cgacaccccc gaaagcgcgc
      901 aataaattat gcccggcatc aaccgggcat tcttccatta ttcagctgcc accggtttta
      961 acaagccagc atcgagcagt ttacgcgtca accactgctg gcctttaccc gttaattgag
     1021 gcgtcaaccg tatctggtag ccatcttcat catccagcac cacttctttc accgtgaaat
     1081 accccgcgtt gatgtactgc tggaacggca catttttacg tccaccggac gctatcagga
     1141 tgccgttctc ccgtaaccag gcaaacagcg cgttttgctt aagtccaaca acctttgcaa
     1201 aattcccaat caggatccct ttagctactg atacccggtc ggcaaaatcg actttaggag
     1261 cggcggccac cagctgctga tttagctggt gggctttctg ttccagaag
//
LOCUS       DAZFDP010000429         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFDP010000429 DAZFDP010000000
VERSION     DAZFDP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..779
                     /gene="yeeO"
                     /locus_tag="ACHJ1G_005614"
     CDS             <1..779
                     /gene="yeeO"
                     /locus_tag="ACHJ1G_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4767231.1"
                     /translation="AVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASV
                     ESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGV
                     GQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNAL
                     FMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGM
                     FADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            880..955
                     /locus_tag="ACHJ1G_005615"
     tRNA            880..955
                     /locus_tag="ACHJ1G_005615"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:913..915,aa:Asn,seq:gtt)
BASE COUNT          304 a          263 c          342 g          398 t
ORIGIN      
        1 gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta aggatttcgt
       61 taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg gggattggta
      121 ttccagcgag tgtcgaatca gtgttattta ccagtggtcg gttattaacc caaatgttcg
      181 ttgccgggat ggggaccagt gttattgccg gaaattttat cgcgttttca attgcggctc
      241 ttatcaactt acccggaagt gcgctcggct ctgcttctac gatcattaca ggccgaaggt
      301 taggggtagg gcagatagcg caagcagaga ttcagttgcg gcatgtcttc tggctgtcca
      361 ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt
      421 tttacaccca ggatccacag gttaaacatg tcgttgtgat tctgatttgg ctaaatgctt
      481 tatttatgcc tatttggtcc gcctcatggg tgctacccgc tggatttaag ggggctcgtg
      541 acgcccgtta cgctatgtgg gtttctatgt tgagcatgtg gggttgccgg gttgtcgtcg
      601 gttatgtgct gggcatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg
      661 ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctat
      721 ggaaataccc tcgacccgaa ccgcaaaagt gtgaaaaaaa gccagttgtg tcggaataaa
      781 caacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt
      841 gtgggtgggc atcgctaata ttcgcctcgt tctcacgatt cctctgtagt tcagtcggta
      901 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt
      961 tagttttgag acatctctat gagtctcaag gcagttaaaa aataaaaatt atttttaccc
     1021 ggtcagtttg taaaaaagtg gccgggtttt ttgatttctg tgtttcgtgc gcttaatcga
     1081 gtctcacgct tcagtgagca aattgctcct ccccaacaga catcacgatt cacaaggtca
     1141 aaaattctgt tccatgaagt accgtacaaa gataaagaga aaaatgcctt atgcgggatg
     1201 tagctgtcgc gtctttatcg ggaaactata cgtaattgcc actttgtctt cggtaatcag
     1261 aaaaagcaga cctgccattt taataaatat tatttctctg gggacgg
//
LOCUS       DAZFDP010000430         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFDP010000430 DAZFDP010000000
VERSION     DAZFDP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1285
                     /gene="rhsB"
                     /locus_tag="ACHJ1G_005616"
     CDS             <1..>1285
                     /gene="rhsB"
                     /locus_tag="ACHJ1G_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4767232.1"
                     /translation="RVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKR
                     VVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFY
                     YNHHSQLTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDAT
                     GSRKTMTWSRYAQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIA
                     VKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAG
                     RVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHY
                     DEADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTV
                     HHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAV"
BASE COUNT          321 a          380 c          395 g          189 t
ORIGIN      
        1 ggcgggtgac agaacagcta aacccggcag gcttaagcta cacgtatcag tatgagaaag
       61 accacatcac catcaccgac agcctggacc gccgtgaagt gctgcacacg cagggcgaag
      121 ccgggctgaa gcgggtggtg aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt
      181 ttgacgccgt gggcaggctc aggacacaga cggatgccgc aggccggaca acagaataca
      241 gcccggatgt ggtgacgggc ctcatcacgc gcatcaccac gccggatggc agggcatcgg
      301 cgttttacta taaccaccac agccagttaa cgtcagccac cgggcctgac gggctggaaa
      361 tacgccggga atatgatgaa ttgggccgtc tgattcagga aactgcccct gacggcgata
      421 tcacccgcta ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca
      481 ccggcagccg gaaaaccatg acgtggagcc gttacgctca gttgctgagc ttcaccgact
      541 gttccggtta tgtaacccgt tatgaccatg accgcttcgg gcagatgacg gcggtgcacc
      601 gcgaggaagg cctgagccag taccacgcat acgacagccg tggacagtta attgccgtga
      661 aagacacgca gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg
      721 tcattgcccc ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccgtcc
      781 gtaccacgca gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc
      841 gcctgaccag tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga
      901 tacaggaaac cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac
      961 ttatccgcag cgaggatgag gggctggtca cccactggca ctatgacgaa gcagaccgcc
     1021 tcacgcaccg caccgtgaag ggtgaaaccg cagagcggtg gcggtacgac gaacgcggct
     1081 ggctgacaga catcagccat atcagcgaag ggcaccgggt gacggtgcat tacgggtatg
     1141 atgagaaagg ccgcctgacc ggtgagcgtc agacggtgca tcacccgcag acggaagcac
     1201 tgctctggca gcatgagacc agacatgcgt acaacgcgca ggggctggcg aaccgctgta
     1261 taccggacag cctgcccgcc gtgga
//
LOCUS       DAZFDP010000431         1284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFDP010000431 DAZFDP010000000
VERSION     DAZFDP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHJ1G_005617"
     CDS             <1..80
                     /locus_tag="ACHJ1G_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767233.1"
                     /translation="RGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(116..1258)
                     /locus_tag="ACHJ1G_005618"
     CDS             complement(116..1258)
                     /locus_tag="ACHJ1G_005618"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO4767234.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          403 a          257 c          212 g          412 t
ORIGIN      
        1 tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat
       61 tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt tttatttatt
      121 caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa ctgtagtgtt
      181 caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc gctaacgttt
      241 ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg aatcccgtag
      301 tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca ggcttaccag
      361 cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa ggaatgacga
      421 ctaaatctgc cagtgggtaa taacaatgca tcttttcagg cggtatgctt cctaacataa
      481 tacaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat gcacctttgt
      541 cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt ttgtgagcag
      601 cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg acgatacgcc
      661 cagcataaaa aataattttc tcttcagggg agatattaag ttcgcttcta gtgatcggct
      721 gaaaattaga ttgataggtt tctaaatcaa ttccatttgg tacaatctca atatctgcat
      781 ttgctaaata agattggtaa tactttttca agtataaact aggcactatc attttaacat
      841 tttgctcaag caattttggt tcaaatgcat tatgcatatg tataaccact cttgcctgag
      901 gagctcgctt acgaatttga gtgtatagct tcatgctatt atgaactaca ataacacttt
      961 ctttcgttat aggaaaatca tgtgcaatat tcaatattct ctgtgaatat ggaagaggat
     1021 caagtcttgt ccatttttga aataaccgct tatatatgcg actgaatcca accctatgaa
     1081 tactacaatt atcattaaca aaggtgtaat ttgagtatcc tggatttttg atacaaacaa
     1141 tacgatttgg aaaattggta cgctgagcaa cctgatacat ccacgtttca acagcagccg
     1201 ctccgcgagg aggaattgaa aaaataggag taaccgtaaa tattatttta tcaaccatct
     1261 cgtatattaa cctttcataa catt
//
LOCUS       DAZFDP010000432         1280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFDP010000432 DAZFDP010000000
VERSION     DAZFDP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..277)
                     /locus_tag="ACHJ1G_005619"
     CDS             complement(<1..277)
                     /locus_tag="ACHJ1G_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4767235.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMA"
     gene            complement(277..618)
                     /locus_tag="ACHJ1G_005620"
     CDS             complement(277..618)
                     /locus_tag="ACHJ1G_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767236.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(611..>1280)
                     /locus_tag="ACHJ1G_005621"
     CDS             complement(611..>1280)
                     /locus_tag="ACHJ1G_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4767237.1"
                     /translation="QRADWQKGLQEGLRNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
BASE COUNT          259 a          453 c          284 g          284 t
ORIGIN      
        1 ccgccatccc ggtgacaagg ccaaacagat tggacaccgt cagcgacggg cgggcactgc
       61 tgccccggcc gttcatctca aagccgctgc cgtcaatcgg gtatgcctca tacttacgcc
      121 cctgccaggt gaccggctcc cctttttcat tcagctcatt acagaaaaaa taccgctcac
      181 caccctgtac cgtcaggtcg atttcccaga gtaccacccg cggtgactgc tctgacttaa
      241 ccgactcgtt cagactttct tcgcgaatat cctgcatcag ttcaccacct gctcaatcgt
      301 acaactgaaa tcactgtacc tggcgttatc tgtgacgctc cactcccggc acaccaccct
      361 caccgtccgg ttatgtttcg gcggtcgcca caaaaaggca cggtaaccac catgccagga
      421 taaaaattca tccagccagc gccgggttgg ttcatccgtc acccggaaca ccgcctgaaa
      481 cgtcttcagt ctggcattaa gtcccgtcgg tcggcgctgt tcataaccgt caccaaaccg
      541 aaccctcacc accgacggtt tctcactcac ctgcatccct tcacgcggga ccagatgcag
      601 cgtttttatc tcagccactc agcattcctc cgtcacgtcg catggacagc atcaccgcct
      661 gcacccgctg gtcaatcagc tgcacaagac tgcctgccgc ctccgcccct atctgtccgt
      721 tagccccgtc attctgaatg gcgatgtggt agaccgggga atacaccaga ccggcactgc
      781 cgttcatact gcccaccgcg cgtacgccca gcgagccatc cgccgcccgg gtcaggggca
      841 taatggcttc aggtccggct tcccccatca gcccggcccc ttttgcaaac gcaaagtacg
      901 tgggcgtgtc cacaatgctg ttgctgtacg cactcaggtt tgccgaggta tacacgccgc
      961 cttttgcatt ggccaccgca ccgcccagcc agtcaccaat gctgccaata aatcctcccg
     1021 caccggacat accgtttgcc gccgtcttaa ttccgttgac aatcgcggca ttcataagaa
     1081 cttttgagat ttcctgcagt acggatgagg cccagttgcg ccattccact ttgtttccgt
     1141 tcagcatctc cgtgatgtta ttcaccatcc ctgagatacc ctccgtcgca agctgtgctg
     1201 cctgtgaagc gtaatcggat gcattgtcca cccagttacg gagcccctcc tgcaagcctt
     1261 tctgccagtc cgcacgctgc
//
LOCUS       DAZFDP010000433         1279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFDP010000433 DAZFDP010000000
VERSION     DAZFDP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..559
                     /locus_tag="ACHJ1G_005622"
     CDS             26..559
                     /locus_tag="ACHJ1G_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4767238.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GTGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTDPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            718..855
                     /locus_tag="ACHJ1G_005623"
     CDS             718..855
                     /locus_tag="ACHJ1G_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767239.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            857..1219
                     /locus_tag="ACHJ1G_005624"
                     /pseudo
     CDS             857..1219
                     /locus_tag="ACHJ1G_005624"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1191..1262
                     /locus_tag="ACHJ1G_005625"
                     /pseudo
     CDS             1191..1262
                     /locus_tag="ACHJ1G_005625"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
BASE COUNT          323 a          305 c          368 g          283 t
ORIGIN      
        1 aatcgataaa tcctgataaa tatccatgaa cgcaaaaatc agatacggcc tgtcggctgc
       61 cgttctggcg ctgattggtg caggggcgtc tgcgcctgaa atcctcgacc agtttctgga
      121 tgaaaaggaa ggtaaccaca ccacagcata ccgtgatggt acgggtatct ggaccatctg
      181 ccgcggtgcc atcctggtgg atggtaaacc tgtcgtcccg ggcatgaagt tgtcgaagga
      241 aaaatgcgac caggttaacg ccattgagcg tgataaggcg ctggcatggg tggagaaaaa
      301 catcaaagtg ccgctgaccg acccccagaa agcggggatc gcgtcattct gtccgtacaa
      361 cattggtccc ggtaagtgtt tcccgtcgac gttttacaga cgaattaatg ctggtgaccg
      421 caggggagca tgcgaggcga ttcgctggtg gattaaggac ggtggcagag actgccgtat
      481 tcgctcaaac aactgctacg gtcaggtctc acggcgtgac caggagagcg cgctggcgtg
      541 ctggggaatt gacagataag cagaatattt tgctgaaaaa tgcggtttgc tcacacgggc
      601 ggataacacg aaatcctgcg aactggcaaa aactaagtga ataaaagtaa aaccccgttt
      661 gttggccgca agcggggttt tgtgtttcct gactccggaa aagtcaaagg agaaagtgtg
      721 tttgatttta gcaaactgat tcgggagatt cgagtgatgg ctgaaaaatt atccacctgg
      781 aagttcatcc ttatctggct ggtgtttgtg attatggcct ccggttattt catcggtcag
      841 atacgctggt ggtgaaatga accgcgtact gtgcgtggtc atcattgccc tgctggtggc
      901 ctgtggtgcg cttagtctgg ggctgaatca ttaccgtgat aacgccataa cctacaaaga
      961 gcagcgcgat aaaaaagtca gtgagctgga gctggcaaat gcaaccatta ctgatatgca
     1021 gcagcgccag cgtgatgttg ctgcacttga tgccagatac tcgagggaat tagccgatgc
     1081 gagagctgaa aatgaaactc ttcgcgctga cgttgccgct ggtcaccgca gcctgcggat
     1141 caacgccacc tgtccaggtc ccgtgcgtga agccaccggc accgcccgcg tggataatgc
     1201 aaccggcccc caactggcag acaccgttac acgggattat ttcaccctca gagagcggct
     1261 gatgacgatg cagaagcaa
//
LOCUS       DAZFDP010000434         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFDP010000434 DAZFDP010000000
VERSION     DAZFDP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..369
                     /locus_tag="ACHJ1G_005626"
     CDS             13..369
                     /locus_tag="ACHJ1G_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO4767240.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            353..>1260
                     /locus_tag="ACHJ1G_005627"
     CDS             353..>1260
                     /locus_tag="ACHJ1G_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4767241.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPS"
BASE COUNT          330 a          328 c          349 g          253 t
ORIGIN      
        1 cgaaaaataa gcatggcaag accaccgaaa gcccccgcct acctggatga catcgccgtg
       61 aagcagtggc gggaaaaatc gcggcagctt gcggaacggg gagacctgac ccccgccgac
      121 tggagcaatc tggaactgta ttgtgtcaac tactccattt accggaaagc cgtcgcagac
      181 cttgcggcgc gcgggttcag cattgttaac agtcagggcg gcgagagcag aaaccccgca
      241 ttaagcgcga aatcagacgc tgaaagggtg atgataaaaa tggcctcctt gctcggtttt
      301 gacccgataa gccgccgtaa aaacccaccg gaaacagaag aagaggacga gcttgaccgc
      361 ctggaataag tacgcagaag acgtaaaaac gggcaaaatt ccggcctgta aacggctgaa
      421 acaggccgtt aaacggtact tttcggacct taaaagcccc ctttacacgt tcgatcgtga
      481 ggttgtggag cggtttattg ccttttccag ggtgtgcccg cacgtaaaag gaccgatgcg
      541 tggcagaccc attgagctgg agccgtggca gcagttcgcc tttgcgtgca tcctcggctt
      601 taaggttaag gccaccggac ggcgcaaata cacgagcgcc tttatcgaag tgccgcgcaa
      661 aaatgcgaaa tccacgaccg ccgcgattct ggctaactgg tttctgatta tggagaacgg
      721 tcagcaggat atctacaccg cagcggtgag ccgtgaccag gcgcggatcg tctttgatga
      781 tgcgcgtcag atgtgccttt tatcccgacc gttacgcagg cgggtgaata ttcaggcgca
      841 taaggtgata cacccgaaaa gcaacagcct gttaaagccg ctggcagcaa aagcggcaac
      901 catcgaggga acaaacccaa gcctttccat cgtggacgaa taccacttgc accccgataa
      961 cggcgtttat tccgcgcttg agctggggat gggcgcacgt cccgaagggc tgttatttgc
     1021 cattaccaca tcaggcagca acgtcgtttc agcctgtaaa cagcactatg attattgctg
     1081 ccagatcctg gacggcgaag aggttaacga ttcaattttt gtactgattt acgagctgga
     1141 cgacgaaaac gaggttgatg atccggcgat gtggataaag gcgaacccta atattgatgt
     1201 ttccgtcgat cgtgaaaaac tggcctcaac catccagaaa gcgcggggta ttccgtcgca
//
LOCUS       DAZFDP010000435         1250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFDP010000435 DAZFDP010000000
VERSION     DAZFDP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..599)
                     /locus_tag="ACHJ1G_005628"
                     /pseudo
     CDS             complement(60..599)
                     /locus_tag="ACHJ1G_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          383 a          296 c          233 g          338 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 actgaacgcc atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa
      121 atgaatttat acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg
      181 agggtttctg actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct
      241 tttttctgag cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat
      301 ctggaatata actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg
      361 gtttttcgtt ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt
      421 gtattcagtc tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca
      481 gacgctgcca caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca
      541 cgaatgagtg gaggcagcgc actcagggga aactcatcac aaggctgacc gaatgacata
      601 gtaacctcac tacacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata
      661 gcagtcaaat caatatgagg taaaattttt tgttgattta atggttaaat aataaccacg
      721 gtgtattttg ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttcgga
      781 gcacatccac tattcaaatt ttacacaacc acgtacgaac aaaaagcatc atcccgggta
      841 ttgcagatac cggtttcctg ccgggaagaa tcataaacag aacaccggga ataacgcctt
      901 caggaatcac cccccctcct gaagaagaat aaaataaaga agactacagt tcctgcactg
      961 aatgctgagt acaatattct atggacagga tgacattttt ataaataaca aaaaccattt
     1021 aatactgata catcaataaa atacttcttc cctgctgaca cagacaaaat gcaccatttt
     1081 ttcttcaaat acttctgtat aatcgccagc caatgatgag aatggattaa aataacactt
     1141 cttcgacaaa cacaaaaaca gccttcattt tacatcatca tccagtttca gttaaccccg
     1201 gacgagcgac agtgtttcag tacaggaatt aaaagccagc ttttgctcag
//
LOCUS       DAZFDP010000436         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFDP010000436 DAZFDP010000000
VERSION     DAZFDP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..1153
                     /locus_tag="ACHJ1G_005629"
     CDS             173..1153
                     /locus_tag="ACHJ1G_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO4767242.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          305 a          304 c          369 g          263 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc gcaagctggc
     1081 acaggtggcg tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac ggcataatcc
     1141 ggtagcggcg taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg cgctgtgtct
     1201 ggcatgcctg caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAZFDP010000437         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFDP010000437 DAZFDP010000000
VERSION     DAZFDP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(834..>1214)
                     /locus_tag="ACHJ1G_005630"
     CDS             complement(834..>1214)
                     /locus_tag="ACHJ1G_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767243.1"
                     /translation="ARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNHYTE
                     SLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPFNIII
                     VICTKEHIERLELMYQGKSDGFFT"
BASE COUNT          349 a          249 c          200 g          416 t
ORIGIN      
        1 accacttcct aggttacttc ctaaatagat cggaaatcat ttcctaggtg ttatttattc
       61 ttatcgggta tcagttttga taggggtatc agacttgata ggggtaacag aattctgtgg
      121 gggtagttca ctatgaccta cccctagctc actgtgagct accgttacat tcaatcatct
      181 tccgatgtgt aatatcgtta cattcaataa ttagtctatg taacatatcg ttactaccaa
      241 ctttcttaca aagagcattt cagatagctg aatgtaacct ctcgttacat ccatttgtca
      301 catagcactc tgtgtaatgc ctttctatgt gtgtgccata atccccccta tattccctgt
      361 gtaaatcata gaatatccct aacttattcc tgtgtaattc catactaaaa attcaatgaa
      421 aaaagtagct taaaagttca attcttactt tcttcacttt tacatcactt tcgcaaatcc
      481 ttgtgtaatg cggcttacag agcaccatca gaaattgttg gctaaaaatt gttctgcttg
      541 tttcttaatg agttttcttg tttgtgcaaa ttttaaaatt tattttgttt ttacagcctt
      601 atttcgtgta atgcgtggca tagcaagccc ttttcttgtg ttgtcaagac atttggcaac
      661 gttcaaacat ctgtttagaa atgccaaatt gttgatctaa caaataaacc tgtataaaca
      721 gaacaccatt taatctattc ccctgtgtaa tccactacat cacacgataa aaacaaaaaa
      781 cttttaatta tatttcatag cacacaaaag tcaacagaag ccctctcgtg ctcctacgta
      841 aaaaatccat cacttttccc ctgatacatc aactcaaggc gttctatgtg ttccttagtg
      901 catattacga ttattatgtt aaatggattt tggcctatat tttgacctgc acccgcataa
      961 acactggttt catcgcttac agcccttgtg agacgtggtg ctgtttctag catctgatcc
     1021 gacataaaac cgatcattct cgtgtttagt gaaagtttag tgagagacat tacagaaagg
     1081 ctttctgtgt aatgatttaa taggtaaaaa cgatgttgat tacgcaagaa aattccttgc
     1141 tgatatttgg ggattctgga ttttccagat cggggggcgc tgaaaatcgc gttaagcact
     1201 tctcgcaatc tagc
//
LOCUS       DAZFDP010000438         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFDP010000438 DAZFDP010000000
VERSION     DAZFDP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..161
                     /gene="cesL"
                     /locus_tag="ACHJ1G_005631"
     CDS             <1..161
                     /gene="cesL"
                     /locus_tag="ACHJ1G_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO4767244.1"
                     /translation="APERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTFQQHVQII
                     ERVRRMTS"
     gene            158..>1210
                     /gene="escV"
                     /locus_tag="ACHJ1G_005632"
     CDS             158..>1210
                     /gene="escV"
                     /locus_tag="ACHJ1G_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4767245.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAE"
BASE COUNT          297 a          222 c          278 g          413 t
ORIGIN      
        1 ttgcccctga acggacgcag ggagtgattg aacatatttt tgtctacgat aacaaattgt
       61 gcttaagttg tttgttaacc aatattgatg tgttttctct aatgaatact tttcagcagc
      121 atgtgcagat tattgaacga gttcgcagga tgacatcatg aataaactct taaatatatt
      181 taaaaaagca gagtcatatc acgatcttat tctggctctc ttcttcttta tggctgtaat
      241 gatgatgatt attccattgc caacagttgt ggtggatatc attatcgcga ttaatatatc
      301 gacagcgttg cttttattaa tgctctcaat ctatataaaa aatcctcttg agctaacttc
      361 ttttcctaca atcctgttga ttacgacgtt gatgcgcctg tcgctcagtg ttagtacaac
      421 cagacttatt ctgctgcatc acgatgcggg tgacattatt tactccttcg gtaactttgt
      481 tgttggcggt aatattgttg ttggcctggt tatttttacc attatcacca ttgtacaatt
      541 tatggttatt acaaaagggg ctgagcgtgt tgctgaagtg agtgctcgtt tttcccttga
      601 tggtatgcca ggaaaacaaa tgagtattga tggtgatatg cgcgcaggcg ttattgatcc
      661 gcttgaggca aaagttcttc gctcccgagt gcaaaaggaa agccagtttt atggttcaat
      721 ggacggggcg atgaagtttg taaaagggga cgctattgcc ggtatcatca ttgttttagt
      781 taacctgttt ggtggcgtgc tcattggaat gtggcaattt gacatgccat ttagtgaagc
      841 acttagcctg ttttctgtat tgtctgtcgg tgatgctttg gttgcccaga tccctgcgct
      901 tattatttcc gtcactgcgg gggttgttgt tactcgtgtt cccggtgaaa gcgaaaaaga
      961 agaaaacctt gcaggtgata ttgttcaaca ggtttctgtt aatagtcgcc cttttttaat
     1021 cagtgccgca ctcatgatcg taatggcgat tattcctggc tttcctacat tggtcttctt
     1081 gtttctggcg gtttgcctgt tgggtatcgc ctggaaatta caaaagaaaa gaacgtttgg
     1141 aaccggaaat aataaggacg ctatgggagc tgatttgtct aatagtcaaa atatctcacc
     1201 gggtgccgag
//
LOCUS       DAZFDP010000439         1208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFDP010000439 DAZFDP010000000
VERSION     DAZFDP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1137
                     /locus_tag="ACHJ1G_005633"
     CDS             <1..1137
                     /locus_tag="ACHJ1G_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO4767246.1"
                     /translation="GLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFGLWN
                     PTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEIRTP
                     DNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTSKLS
                     ATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALGLKD
                     THSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQSFN
                     VPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAPEDR
                     PNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          402 a          258 c          222 g          326 t
ORIGIN      
        1 ggacttaata ataattccgc atctttagtt ttagatgctg caattagaat taattctgat
       61 tttaaaaagc agtggaatga tatgtcatgc gctgaaaaat tactaaaagt tcttagtttt
      121 ggtttatgga atccaacgta tactcgtagt gaaaggcaaa catttcaaga gttgttaacc
      181 gttttagaac ctgtatctcc cgcgcctaat gaattgggca gaatatatgc taattttgca
      241 gatggctcat ccttaagaat ttccgtcact aacagcgaac ttgttgaagc cgagattcgc
      301 acaccagata atgaaaagat tcttatgctc ctggagtcaa acgaacaaaa taggttatta
      361 caatctttac ccatcaatct ccacatgcca tacattcagg ttcatcgtgc cttatctaag
      421 atggacctga ctgatcataa gtcaatgcac aatctactta gttttacgtc aaaactatca
      481 gcaaccttga ttcctcataa tactcaaaca gatccgcttt ccgggcctac accattcagc
      541 tctatgttta tggatacatt tcgaggactc ggcaatgcaa agctttcact caatggtgtt
      601 gatatacctg tagatgcaca aaaattgctt cgcgatgcac taggacttaa agacacacat
      661 tcatcacttg cccggaatgt tataaataat ggtatttctc gtcatcatgc aaagcaaata
      721 gcaagggaaa gcagcggcag cgataaacag aaagctgaag ttgtggagtt tttatgccac
      781 cccgaagcgg caacagccat atgctcggct ttctatcaat ctttcaatgt gccagcctta
      841 atgttgacac atacaaggat ctctcaagcc agagaataca atgtggaaag atcgttagat
      901 gtacctaacg cgtgcattaa cataagtatc tctcaatcac cagatggaag cattcacgtt
      961 gccagccata ccgggattct gataatggcg ccagaagacc gccccaacga gttaggaatg
     1021 ttgacgaaca ggacctccta tgaagtgcca caaggtgtga aatgtgaaat cgatgagatg
     1081 gtaagaacgc tacaaccaag gtatggcgca tctgaaacat acctgaaaaa catttaaaaa
     1141 taaaaaaatg gacaccacac actttattta tcaaaacaaa caataactaa tcacactcac
     1201 aattatta
//
LOCUS       DAZFDP010000440         1186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFDP010000440 DAZFDP010000000
VERSION     DAZFDP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..311
                     /locus_tag="ACHJ1G_005634"
                     /pseudo
     CDS             150..311
                     /locus_tag="ACHJ1G_005634"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            295..360
                     /locus_tag="ACHJ1G_005635"
     CDS             295..360
                     /locus_tag="ACHJ1G_005635"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO4767247.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            476..733
                     /locus_tag="ACHJ1G_005636"
     CDS             476..733
                     /locus_tag="ACHJ1G_005636"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4767248.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(780..>1186)
                     /gene="nleF"
                     /locus_tag="ACHJ1G_005637"
     CDS             complement(780..>1186)
                     /gene="nleF"
                     /locus_tag="ACHJ1G_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO4767249.1"
                     /translation="ARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALR
                     AGMTIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAM
                     KCLVEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          326 a          291 c          254 g          315 t
ORIGIN      
        1 cactatgagt caactattta gatgatatta gtgacctgtt tcagagcatt agcgcaaggc
       61 gattctttgt cttcttgcac taatttttta tcataaaaat gttcctagca ctgggcatca
      121 atatcgcagg tcagaaagag ctcctgggga tgcggctggc cgaaaatgaa ggggcgaatt
      181 tctggttcaa tgtgctgact gaactgaaaa accgcggtct gaacgatatc ctcatcgcct
      241 gtgtgtatgg cctgaaagaa ttcccggagg cccgcatcca gttatgcatc gtgcatatgg
      301 tgcgcaacag catgcgcttc gtgtcatgga aggaatacaa agccgtcact cgcgacctga
      361 aagcgattag cctcccacag aagaggcagg ccagcaggca ctggaagcgt ttgctgcggc
      421 ctgggactgc cgctatccgc agataagccg gtgctagctg tcaaactgga ctaacttggc
      481 gacgtttttc gcttatccgg cagatatccg caaagtgatc tacacaacga acgccatcga
      541 gtcgctgaac agtgtgatcc ggcatgccat caggaaacgc aaggtgttcc cgacggacga
      601 gtcagtaaaa aaagtggtgt ggctggcaat ccaggccgcg tcacagaaat ggacaatgcc
      661 gttaagggac tggcgaatgg caatgagccg ctttattatc gagtttggtg accgtcttga
      721 cggtcacttt taagaaaagg catttacaca gaatcctaaa caggctcaca ggcctctaat
      781 catccacatt gtaaagatcc tttgttgtaa gtaagatctg gtaccctaat aatatcaaaa
      841 ggattaaaat catcactgca tccttccacg aggcatttca ttgctcgtag atcaccatat
      901 agctctgaag agtgatgcgg acatagcaga ttataaactt tacatttcga atcttctttg
      961 ccttgtctac tttcctcaat ttttactttc aatgaatgca gaaaaaaaag atccctgata
     1021 taactatccc tgtcctctat cgtcattccc gccctcagcg ctccgacaac ctcatcagta
     1081 ttttcatttc tcacctcaaa tacactattt ctcttatcca tcagcccact tacaaaagca
     1141 cttagctctt gaaaaatttc ctcaagctca ttattatttc tggcac
//
LOCUS       DAZFDP010000441         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFDP010000441 DAZFDP010000000
VERSION     DAZFDP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            388..495
                     /locus_tag="ACHJ1G_005638"
     CDS             388..495
                     /locus_tag="ACHJ1G_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4767250.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            871..978
                     /locus_tag="ACHJ1G_005639"
     CDS             871..978
                     /locus_tag="ACHJ1G_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4767251.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(983..>1177)
                     /locus_tag="ACHJ1G_005640"
     CDS             complement(983..>1177)
                     /locus_tag="ACHJ1G_005640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767252.1"
                     /translation="FRLAADSTTVFLFRNPSLTHSVLRIPQNIIRLQPLHFSYEKGRR
                     VSPLRPGPGANCHYGSLTPA"
BASE COUNT          308 a          271 c          358 g          240 t
ORIGIN      
        1 aagcggaagt agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
      241 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
      361 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      421 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      481 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      541 gatcaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
      601 ggcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
      661 gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg
      721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
      781 atgcacctga caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg
      841 aaacgttgtc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
      901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
      961 ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
     1021 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
     1081 gcaagcggat aatattctgc ggaatgcgca gcacagagtg agtaagtgat ggatttcgga
     1141 acagaaaaac agtcgtagaa tcagcggcaa gccggaa
//
LOCUS       DAZFDP010000442         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFDP010000442 DAZFDP010000000
VERSION     DAZFDP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..430)
                     /locus_tag="ACHJ1G_005641"
     CDS             complement(89..430)
                     /locus_tag="ACHJ1G_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4767253.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
     gene            complement(448..>1176)
                     /locus_tag="ACHJ1G_005642"
     CDS             complement(448..>1176)
                     /locus_tag="ACHJ1G_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4767254.1"
                     /translation="REKGCQWPHSDWHSENMTTMRHAPGAIRLCWHCDNQLRDQFTER
                     LESMATDNCARWVLSVVRRDLGFDDSHVVTMPELCWWLIRNDLADALPESAARKALRL
                     PKPVVPSVTRESDLVPSVPATSIIQDKAKKVLALKVDPESPESFMLRPKRRRWVNEKY
                     TRWVKTQPCACCGKPADDPHHLIGHGQGGMATKAHDLFVLPLCRKHHDELHADTVAFE
                     EKYGSQLELIFRFIDRALAIGVLA"
BASE COUNT          306 a          335 c          246 g          289 t
ORIGIN      
        1 tttattacat tgcctcatca taatcagaca aaatattaaa attttattaa agcaacagag
       61 aaaaaaaggg gggtaattcc ccctaatatt acaacattga aagcacacca tccagaaaac
      121 caagagctgt ttgcaactct tttcttattg tcccatcaga acacttctgc ttctttgcaa
      181 tagttcttag tgagatgccg ataacaaaat gagcaataac caactcatac tcttcgggct
      241 tatatttccg caatcgcgca acacagctgt caatcatgat tccttcatca tcatcgcatt
      301 gttggcgtgt tttctttcca tgaggtagta aacctttaaa ccctgcagca acaggctgcc
      361 agtccacacc actgttatct gctgcagccc atgcacccca gcggtccaat acttcataca
      421 tatcacgcat caactttctc cacaaaatca ggccagcacg ccaattgcca gtgcacgatc
      481 gataaaacga aatatcagct ccagctggga gccatacttc tcttcaaatg ccacggtatc
      541 cgcatgcagc tcgtcgtgat gctttctgca caaaggcaac acaaagaggt catgcgcttt
      601 tgtagccatt ccaccctgac cgtgacctat caggtggtgg ggatcatcag caggttttcc
      661 acaacatgcg cacggctgcg tcttaaccca tcgcgtgtac ttttcattaa cccagcggcg
      721 acgttttggg cgtaacataa aagactccgg cgactccgga tccactttca gcgccagcac
      781 ctttttcgct ttatcctgga tgatgctggt ggcaggaacc gaaggcacaa ggtcactttc
      841 ccgggtgaca gacggcacaa caggcttcgg taatctcagt gccttacggg ctgcactttc
      901 cggtaaggca tccgccagat cattacgaat cagccaccag cacagttccg gcattgtcac
      961 aacgtgactg tcatcaaaac cgagatcccg acgcacgaca gacaacaccc agcgggcaca
     1021 gttatccgtt gccattgatt ccagccgttc cgtgaactga tcgcgcagct ggttatcgca
     1081 gtgccagcac agacggattg cgcccggcgc gtgtcgcatt gtggtcatgt tctcgctgtg
     1141 ccagtcggaa tgaggccact ggcagccttt ttcacg
//
LOCUS       DAZFDP010000443         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFDP010000443 DAZFDP010000000
VERSION     DAZFDP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            443..658
                     /locus_tag="ACHJ1G_005643"
     CDS             443..658
                     /locus_tag="ACHJ1G_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4767255.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            658..1155
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005644"
     CDS             658..1155
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005644"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4767256.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT          312 a          245 c          317 g          301 t
ORIGIN      
        1 cataataaaa aaagccagcg actgacctga aagaagacgc tggctaaaag gccttatatg
       61 tttgtagaga cttatttttc acagacagca atgatgcctg tcaatatatt atcaatatgc
      121 ggattgtttc agttacagat gctttattaa ggaaaaaaac agccagcact gactttcggt
      181 ggagaggtgc tggctcagaa ggatagttgg atttcacatg atacttatgc ctggcggtat
      241 attttctggc agacagtgac gggtgttgtc aagatattgt gtcatttata acctgaatca
      301 gggggtggcc ggaatgttat ctggcatttt tagcagagcc tgaatgccat aatcacggct
      361 cccggagttg gccgtcagtg ggtgacactg gcggcttttt tgtttttctt tactttcatt
      421 ttctgtcggc ggtgacggag acatacatca gatggaaaaa atcacaacag gtgtgtcata
      481 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
      541 gtcccagtgg gtggcaatag gtgtgctggg aagtctgctg tttggcctgc tgacgtatct
      601 gacaaatctt tatttcaaga ttaaagaaga taagcgtaag gctgcgagag gtgaataatg
      661 tcgccgtcat tacgcaaggc tgttgcagct gctattggtg gtggggctgt tgccatagcg
      721 tctgtgctca tcactggtcc aggtggtaac gatggtctgg aaggtgtcag ctacatacca
      781 tacaaagata tcgttggcgt atggactgta tgtcacggac acaccggaaa agacatcatg
      841 cccggtaaaa cgtataccga agcagaatgc aaagccctcc tgaataaaga ccttgccacg
      901 gtcgccagac aaattaaccc gtacatcaac gtcgatatac cggaaacaac gcgcggcgct
      961 ctttactcgt tcgtttacaa cgtgggcgct ggcaatttca gaacatcgac gcttcttcgc
     1021 aaaataaacc agggcgatat caaaggcgca tgtgatcagc tacggcgctg gacatacgct
     1081 ggcggtaagc aatggaaagg gctgatgact cgccgcgaga ttgagcgtga agtctgtttg
     1141 tgggggcaac aatgagcagg gtaaccgtta ttatc
//
LOCUS       DAZFDP010000444         1175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFDP010000444 DAZFDP010000000
VERSION     DAZFDP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..192)
                     /locus_tag="ACHJ1G_005645"
     CDS             complement(<1..192)
                     /locus_tag="ACHJ1G_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767257.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCII"
     gene            complement(214..753)
                     /gene="yhaB"
                     /locus_tag="ACHJ1G_005646"
     CDS             complement(214..753)
                     /gene="yhaB"
                     /locus_tag="ACHJ1G_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO4767258.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1009..>1175)
                     /gene="tdcR"
                     /locus_tag="ACHJ1G_005647"
     CDS             complement(1009..>1175)
                     /gene="tdcR"
                     /locus_tag="ACHJ1G_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO4767259.1"
                     /translation="AARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDR
                     TSFCECNRFP"
BASE COUNT          358 a          208 c          223 g          386 t
ORIGIN      
        1 gataatgcat tttccatcag gttgtaacgt gttaagtaaa gtgaccattt caatagaggt
       61 ctgttttggt ttttctatac ctggtgctag tgcttcgagg ttatttcctg ttgggctttc
      121 aggaatgtca ttcatttttc gacggactaa atcactacta atatcattac caattgatga
      181 aacagggaac attattttat cctttataaa taatcaataa aaatcattat taaaatgatc
      241 gggtgaaaaa aagagaattt gccaacaatc tgaagtaatg ctggttttat taaagtgcac
      301 aaggggtgtt aagatggctt cttttcgggt gaattttgca caatacttag aaatcgtctc
      361 cttaatacat tccagctctt ttttattgac ggctgagcaa gtacattgtt cgccaaaagg
      421 tgcaaaagtg cctacatcta atgtttgtat gatataatca tcatgttccc ctgtagaaat
      481 atctatgatg aatggactca tattttccgg ttggattcgg atcattacag aaggaacatc
      541 gccttgatag aaattcaatg cagtaattgc agataatact ctccgcagtt cattacttat
      601 actgtaaatt ttattaacgt cattggtgga gacaatttct gcaaactttc tttttaccgt
      661 ccaataatca atatttctat tgtgattgtt aaccactgcg tgcattggcg ggattgaagg
      721 gtgaaaaata tgtgcaattg ggaagccttt cattgatgcc tgccttgtcg tcatgacaaa
      781 caataataga tgcttatttt cctcgcaggg ttctttcgca ctatcaaaaa acgctcattt
      841 acccggcgtt acagtaaacg aagcaacatt ctgtggccta cgacgatcaa aagctctgtg
      901 aaaatttccc ttgttgtctt tgtctgacct tatcgcggcg accagcaatt attcgttttg
      961 tgatcctcaa cacaaaatgc tattcgtcgc ctatgagggg agaagaattt atggaaaacg
     1021 attgcattca caaaatgacg tcctgtcaga agagaaaaag ttgacgttaa tcaattgttc
     1081 gagatgatca atacctgtat tcgaaatttt aaaatattga taaaataaat gtttgttctc
     1141 caacaaatag cgtgaaaaaa tgtgacgtgc cgcag
//
LOCUS       DAZFDP010000445         1171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFDP010000445 DAZFDP010000000
VERSION     DAZFDP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..396
                     /gene="yqeJ"
                     /locus_tag="ACHJ1G_005648"
     CDS             <1..396
                     /gene="yqeJ"
                     /locus_tag="ACHJ1G_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO4767260.1"
                     /translation="KMIYNETCTANWVIFNDQGRANLTIDFMYNKKNKTGTVALSGTW
                     QQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLAQLIPDFYVFPE
                     KSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(429..509)
                     /locus_tag="ACHJ1G_005649"
     CDS             complement(429..509)
                     /locus_tag="ACHJ1G_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767261.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(545..970)
                     /gene="yqeK"
                     /locus_tag="ACHJ1G_005650"
     CDS             complement(545..970)
                     /gene="yqeK"
                     /locus_tag="ACHJ1G_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO4767262.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          426 a          199 c          185 g          361 t
ORIGIN      
        1 aaaatgatat ataacgaaac atgcactgca aattgggtaa tttttaatga ccagggacga
       61 gcaaatctta ccatcgactt tatgtataac aaaaaaaata aaacaggaac tgttgcgttg
      121 agtggaacct ggcaacaagg taacagagaa agtaaatcaa tacggcgaaa tattgaatac
      181 acatggattg aaaactatga cacagcccat ttaacatcaa agaaagttaa taagtttgag
      241 attatggatc aagttgatga tgatagactt gcacaactta ttcctgattt ttatgtcttt
      301 ccagaaaaaa gtgtaagcta taatatacta aagcaaggta agcatgcttt tattttgagc
      361 attggtaaca gagcaataat gcattgtgca aggtaaacta aaatagataa caaaaagatg
      421 gaacctcgtt aatcataaaa caataaaatt aaatattctc gcagtatatg gcagtctaaa
      481 gcatcaaagt tttgatcaac atctttcatt ttagacatct ccttgcaatg taaaatatca
      541 tgtatcaaca acatctggtg caaatccatg agtcgaactc gacattcatc ttagctcgat
      601 tattattcct tcgtacagtc gatgtaaaca atacagaaag aggattatta agaaccagtt
      661 tactcagtga atcaatagag gaaaggacta acgtttcttt aaaagaattg atttcatcat
      721 ctgttaaact aaactcatca ttgacagatc gtgagatata actgctttta actttactct
      781 ttacgttgac tttattgaca gaattaacat tcacaaatct tgaatttaat gcccatgatg
      841 ttgtttgcgt gagaaccttc tcagcattaa ggaatttttt taacggaatc cttggtttct
      901 ttttcgagtc cgaatttaca atatcatgca tatagaccat ttcatgaatc tgcgaaaact
      961 caatgtccat tatatacctc acatttttac cgtgactcga tgttactgtt caataatcac
     1021 cttccatcaa tactaaaatt aataccccta atgtgccgat aacaaatata gtcattctac
     1081 gtaatgtctc cataaggtga tatttgacat tatcagaagc tgcgaattcg gattttgctc
     1141 taatcaaatg agagaaatgt aaaatgaagc c
//
LOCUS       DAZFDP010000446         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFDP010000446 DAZFDP010000000
VERSION     DAZFDP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..1159
                     /locus_tag="ACHJ1G_005651"
                     /pseudo
     CDS             58..1159
                     /locus_tag="ACHJ1G_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          419 a          160 c          277 g          303 t
ORIGIN      
        1 tacaggtgat caaaatttaa cctttggaat aactaaaaag ataaaaaagg acgccaggtg
       61 agtattcaaa gtttgcttga ttatatttca gtgatccctg atatacgaca acaaggaaag
      121 gttaaacata aattatctgc tattttgttt ctcaccgtat gtgcagtaat tgcaggtgcc
      181 gatgagtggc aggaaattga agattttgga catgaaagac ttgaatggct aaagaaatat
      241 ggtgattttg ataatggcat tccggtcgat gacaccattg cacgcgttgt gagtaacatt
      301 gacagtttgg cctttgaaaa gatgtttatt gaatggatgc aggagtgcca tgaaatcact
      361 gatggtgaaa ttatagcaat agatggaaag accataagag gctcctttga taagggaaaa
      421 agaaaaggag caatccatat ggtgagtgca ttctcgaacg aaaatggtgt tgtactgggg
      481 caggtgaaaa cggaagccaa aagtaatgag attacagcca ttccagagtt gcttaaccta
      541 ctgtatttaa agaaaaattt gataaccatt gatgctatgg gctgtcagaa agatatcgct
      601 tcgaagatca aagataaaaa agcagattat cttctggcag taaaaggcaa tcaggggaaa
      661 ttacatcatg cattcgagga aaaatttcct gtaaatgtgt tttctaatta taaaggcgat
      721 tcgtttagta cgcaggagat aagtcatgga agaaaagaaa cacgtttgca tattgtcagt
      781 aacgtaacgc ctgaactttt gtgattttga attcgaatgg aagggattaa aaaagctttg
      841 tgtagcattg tcattcaggc agaagaaaga agataaatca gcagaaggtg taagcatccg
      901 atattatatt tcatcaaagg atatggatgc taaagaattt gcacatgcta tcagagcgca
      961 ctggctgatc gagcacagtc ttcattgggt gttagatgta aaaatgaatg aagatgccag
     1021 ccggataaga agaggaaacg cagcctaaat aatatctgga ataaagaaga tggcactgaa
     1081 tttattaagg gattgcaaag acattaaggg tgaagaggaa aagaaagaag gctgcgttaa
     1141 acacagggaa agatcatca
//
LOCUS       DAZFDP010000447         1146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFDP010000447 DAZFDP010000000
VERSION     DAZFDP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHJ1G_005652"
     CDS             <1..249
                     /locus_tag="ACHJ1G_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4767263.1"
                     /translation="AGDPDTLLGLPVEYLEFMPDNVIALGDFKRGYYIVDHETGVRTR
                     PDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            289..861
                     /locus_tag="ACHJ1G_005653"
     CDS             289..861
                     /locus_tag="ACHJ1G_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4767264.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            863..>1146
                     /locus_tag="ACHJ1G_005654"
     CDS             863..>1146
                     /locus_tag="ACHJ1G_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767265.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQT"
BASE COUNT          274 a          281 c          341 g          250 t
ORIGIN      
        1 gcgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt tatgcctgat
       61 aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca cgaaacaggt
      121 gttcgcacca gaccggacaa cctcacagag ccgggcttca tcaaaatttt cacgcagaaa
      181 tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggaact gccacaggac
      241 gacgattaac agcatacaga aggggcttaa aagccccttt agtgttttat gggtgaaaaa
      301 attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcgccag caacacgctt
      361 acaggctacg ttgttcgctg ggataagctt tcagagctgc tatgggggga gttttacgaa
      421 aaattccagc ggggagcgtt tactgaatgg cttgcggcgg gtaatgacgt tcgcggcctg
      481 tatgagcatg accacagcat gttgctgggg cgtacccgtt ccggcacgct gaaactggaa
      541 gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagcac agggcgggac
      601 gttatcgaac tggttaaacg tggtgatata tccggcatga gctttggctt tcgttcccgt
      661 aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt tgcggaactg
      721 tacgaaatta ccgtcacatc cgtaccggct taccctgatt ccggcgtgga gctggcccgc
      781 cgctccctgt acgagcagca ccccgaaaaa atgccgcgtg cggataatcg ccgctggtgg
      841 gcggatttag cgggggtgtg atatgtggcc tttcagaaga aaaaaagagc agcgcagcat
      901 gacgcttgat gagtttatgg cgctggctgg cacatcgaac acgggggcgg gtgagtacgt
      961 atcatcgggg acagcggaat cactgcccgc cgtcatgaac gccgtgacgg tcatctctga
     1021 ggcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg ggaaggaggc
     1081 ccgcgagtgg cttgattctc atccggtcga tcacatcctc aacgagcgcc cgaacgcatg
     1141 gcaaac
//
LOCUS       DAZFDP010000448         1145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFDP010000448 DAZFDP010000000
VERSION     DAZFDP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1145
                     /locus_tag="ACHJ1G_005655"
     CDS             <1..>1145
                     /locus_tag="ACHJ1G_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4767266.1"
                     /translation="SDGITKVENAFMAWVGGANEASGVTKTLSGVLNGVAGNIDNVAT
                     AAGALVAVGGARYFGNMASGAVSATAGLVTAARNEVALAEAQLRGTQIATARARAAVY
                     RAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARTAAQNELNSTTAVGSRLMTGAL
                     GLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPEASDNEG
                     RTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLTSVKTVTE
                     GLSQATEQLAVEQSRLAQMQEKVQSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVM
                     NGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKT"
BASE COUNT          275 a          280 c          385 g          205 t
ORIGIN      
        1 tctgacggga tcacaaaggt ggaaaacgcc tttatggcct gggtgggtgg cgcgaatgaa
       61 gccagcgggg tgacaaaaac gctctccggc gtgctgaacg gtgttgccgg taatattgat
      121 aatgtggcaa cagccgcggg ggcgctggtt gccgtcgggg gtgcccggta ctttggcaat
      181 atggcctccg gagcggtgtc tgccacggca ggacttgtga cggcagcacg taatgaagtg
      241 gcactggcgg aagcacagct cagggggacg cagattgcca cggcgcgggc aagggcagcc
      301 gtgtaccgtg cacagcaggc tgtggcggca gcccgcggga cggagatgca gattgctgca
      361 gaagcccgtc tggcggccac acaggaacgc ctgaacagaa atattgctgc cagaaccgca
      421 gcccagaatg agctgaacag tacaacggcg gtgggctcac gtctgatgac tggtgcgttg
      481 ggactggttg gtggcgtacc cggactggtg atgctggggg cagcagcatg gtatacgctg
      541 taccagaatc aggagcaggc cagggagtca gcgcgccagt atgcactgac gatagatgaa
      601 atcgcgcata aaacgccgtc aatgtctttg cctgaagcct cagataatga aggacgaaca
      661 cgggcggcgc tggcagagca gaaccggctg attgatgaac aggccagccg ggtgaaatcc
      721 ctgcaggaaa aaatcgctgg atatcagtat gttctggcta accctggctg gacaaccggt
      781 gacggattca tgataaacca tctgacatcg gtgaagaccg tgacggaagg gctttctcag
      841 gcaacagagc agcttgccgt tgagcagtcc cgtctggcac agatgcagga aaaagtgcag
      901 tccattcagg atgtgcttgc cgggctggaa gaccgtcgtg tggcgttaat tcgtcagcag
      961 gcggcagagc agaataaggt gtaccagtcc atgctggtta tgaacggtca gcatacggaa
     1021 ttcaaccgtc tgctggggct ggggaatgaa ctgcttcagc agcgtcaggg actggtgaat
     1081 gtaccgttac ggctgccgca ggccaccctg gatgataaac agcagagcgc cctgacaaaa
     1141 acaga
//
LOCUS       DAZFDP010000449         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFDP010000449 DAZFDP010000000
VERSION     DAZFDP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..80)
                     /locus_tag="ACHJ1G_005656"
     CDS             complement(<1..80)
                     /locus_tag="ACHJ1G_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767267.1"
                     /translation="MINQLTFTKHYDTFDNVSKIYSDKFP"
     gene            complement(134..304)
                     /locus_tag="ACHJ1G_005657"
     CDS             complement(134..304)
                     /locus_tag="ACHJ1G_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767268.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(324..512)
                     /locus_tag="ACHJ1G_005658"
     CDS             complement(324..512)
                     /locus_tag="ACHJ1G_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767269.1"
                     /translation="MKWFTPEHVVQAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          314 a          221 c          176 g          421 t
ORIGIN      
        1 tgagggaatt tatcagaata aatctttgat acattatcaa acgtgtcata gtgttttgta
       61 aaagttagtt ggttaatcat taaaaatctc cttgatgtaa tcacgctcta tggcgctact
      121 atggtgataa aaatcacttg cttttatttg agagtgtttc agcctctctt ttcaacatat
      181 caagaatgta atctgtgaca gtgatccgtc tacttctggc ttctttaaat aacacctgaa
      241 atacttcatc aggaatatga aagcaagtgc gtggttgtct tttcttttct gtagtttcgc
      301 tcatagtttt ctccttctct tctttactct gtttcagatt ctttttcatt ttttcttagt
      361 tcatcaataa tcttgagtgc ttcattgaag caagctgctc gctctggata acctcgactc
      421 cttgccatat tacgattcat tactacttgg tggcgtgtaa gctcaccttt tttaaatgcc
      481 tggacgacgt gctcaggtgt aaaccatttc ataaaaaact ccttattcag ttaaaagaaa
      541 aaacaccttg caagcctgac ggcttcaaag tgcattgatt ttgattattt agagaagaat
      601 gtttttctgt ccctctaata cctattatta catataaaga aacatttgtc aatacctttt
      661 cataaaaaat ctgatctttt tcatgcactt acattttttc ttagcaattc gtgctctcct
      721 atgtagtgct attcttcctt attgtctaaa acgctctaaa acgcgttcta acgagatttt
      781 gataaaagca atacattccc ttatctaatg gctgtttagc tcgccagagg cttatttaag
      841 tggcttattt tgtatttgtt agatgatgat agatataaaa cagagagtca ctttataaaa
      901 cgctctgtaa agccctgtag tgagtttatt atatgtaggc aatgcgtttc cattccctac
      961 cctgcttaat gttgttagag aagcatttag accccttatt tttaattacc taaagtatcc
     1021 tctatacatc tctgttatga aacacctctg aatccttcat aaaacacatt atgacgcaaa
     1081 gaacactttt ctaaggtgat tcccttcctg aatgctgttt cttgcgtcag ag
//
LOCUS       DAZFDP010000450         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFDP010000450 DAZFDP010000000
VERSION     DAZFDP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..288)
                     /locus_tag="ACHJ1G_005659"
     CDS             complement(<1..288)
                     /locus_tag="ACHJ1G_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4767270.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDM"
     gene            complement(427..660)
                     /locus_tag="ACHJ1G_005660"
     CDS             complement(427..660)
                     /locus_tag="ACHJ1G_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767271.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            774..>1129
                     /locus_tag="ACHJ1G_005661"
     CDS             774..>1129
                     /locus_tag="ACHJ1G_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767272.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEER"
BASE COUNT          305 a          233 c          255 g          336 t
ORIGIN      
        1 catatcaatt tgcttagagt ccccggcggc caccggggat tttttctttg tgatttcatc
       61 aagcgcatac ttaaaagctc tgctaatcgg actgatgtct gatgccatgc caaaagcaca
      121 caagaccgaa gctataaacc tccagtctgt tctgcttatc ttcgattcat gacagccaat
      181 catctttgcc agaccgcgct gggtaagcgt tgacaggttg atgagtaaat cagtttcagc
      241 gcgatcaatt tctcgctgtg atagtttgct gtaacttgtt tgttccattt cttaagattt
      301 ccaatagtga atagctagtt gaaaggtatg cgtggaaacg catatggcct tagttggtca
      361 gatatattgg gactcgcttt gtcagcgacg taggacgaat gtccattgtg aaaatagcgg
      421 tgttacttat gcagccgatg ctctacgcga tacgaacact aggttttcct ttttcacagg
      481 tttataaccc gtgaaattac gagtagcttc ttcgattgca ttcgctttat caggggaagc
      541 tcttcgaaat ccatatgcaa tctggtcaag atagccaact gaagttttcg ctaatgcggc
      601 gagtcgcttc cattcctcac tagaagcctc ttttcgccag cgtagtagtt cattactcat
      661 tagtgcctcc gtttatcaca cagaacaact ttaccatttt gataaatcat ccgcaatgta
      721 aatttatcat attgcgtatt tatccatttg ctaaatagag ggaaaattgt gagatggaaa
      781 acaaagatat tcgcaaatcg aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg
      841 gtaacactaa tgcaagcttt gccgataagc ttggggttag cccttctcaa ctcacgcaag
      901 tctccggtga aaaaagcact cgaaacatag gggataaact agcaagaaaa tttgaagccg
      961 cgcttgggtt acctaatggg tggcttgatt tggtacatga tgtaacacca attgcatcat
     1021 gctcagattc tttaactttt gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg
     1081 aggcaattct tggtgttgat ggtgccatcg agatgaccga agagcgcga
//
LOCUS       DAZFDP010000451         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFDP010000451 DAZFDP010000000
VERSION     DAZFDP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..779
                     /locus_tag="ACHJ1G_005662"
     CDS             <1..779
                     /locus_tag="ACHJ1G_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4767273.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          302 g          270 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggtgtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZFDP010000452         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFDP010000452 DAZFDP010000000
VERSION     DAZFDP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..362
                     /locus_tag="ACHJ1G_005663"
                     /pseudo
     CDS             122..362
                     /locus_tag="ACHJ1G_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            382..713
                     /gene="sctI"
                     /locus_tag="ACHJ1G_005664"
                     /pseudo
     CDS             382..713
                     /gene="sctI"
                     /locus_tag="ACHJ1G_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            710..>1123
                     /gene="sctJ"
                     /locus_tag="ACHJ1G_005665"
     CDS             710..>1123
                     /gene="sctJ"
                     /locus_tag="ACHJ1G_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO4767274.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSG"
BASE COUNT          368 a          193 c          238 g          324 t
ORIGIN      
        1 agatttaatg tgaatttgaa aaatcaggac acaaataata gttcatttag ctacggagat
       61 aaccgattcg agaagtctca aggtagcaac tggacatttc aggaataatt gaggaggact
      121 tatggcagat tggaatggtt atattatgga tatcagcaat caatttgatc agggcgttga
      181 tgatctgaac cagcaagttg aaaaagcgtt ggaggattta gccaaccaat ccctccgaca
      241 cgaaatttct tgccgaatat caaagtgcat tagctgagta tacattatat cgaaatgcgc
      301 aatctaacgt tgtaaaagcg tataaagatc ttgattcagc aatcatacaa aacttccgct
      361 aaactagata aggattttat tatgttagta agcaatatgc cacctattga tagagctgag
      421 caatcaactg ctcatgaaat acagcaagcg aaagtgattg atttaaatga ccgggttctc
      481 aatcttgaca acccggatga taaaatgatc tccgcttttg ccaactatgc tgtacaaact
      541 gaaaattggc agcaaaatgc gttacaagca ttaaggtctg ataaagaggg gttaactcct
      601 gaaaaattat tagtcttgca ggatcatgtg cttaattata atgtcgaggt ttcattggtg
      661 gaacattagc aagaaagatt gtcgctgcag ttgagacgtt gacccgctca tgaaatatat
      721 atcattatta ttgtttatcc tgctgttgtg cggctgcaag cagcaggaat tattgaacca
      781 tcttgatcaa cagcaagcta atgatgtcct tgctgtttta caaagacata atattaatgc
      841 tgaaaagaag gatcaaggca aaacaggatt ttcaatattt gtcgaaccaa cggattttgc
      901 ttctgcggtg gattggctaa aaatctacaa tctaccgggt aagcctgata ttcaaatatc
      961 gcagatgttc cctgcagatg cactcgtttc atctccacga gcggaaaaag caaggctata
     1021 ttctgctatt gagcagcggt tagagcaatc tctcaaaatt atggatggca ttatttcttc
     1081 gagagtacat gttagttacg atgttgataa tggcgatagt ggt
//
LOCUS       DAZFDP010000453         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFDP010000453 DAZFDP010000000
VERSION     DAZFDP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..513)
                     /gene="wzy"
                     /locus_tag="ACHJ1G_005666"
     CDS             complement(<1..513)
                     /gene="wzy"
                     /locus_tag="ACHJ1G_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFO4767275.1"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVII"
     gene            complement(579..>1123)
                     /gene="wzx"
                     /locus_tag="ACHJ1G_005667"
     CDS             complement(579..>1123)
                     /gene="wzx"
                     /locus_tag="ACHJ1G_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O26 family O-antigen flippase"
                     /protein_id="HFO4767276.1"
                     /translation="GTLASPVATSLYATAEKIIKCIQSIATPLNQYYFTRLIKQHELK
                     LEPYKVGEYKSLLYASTNIQLKFMVFIVLSLGGVGTILGYKVQSIAEIRSAFIPLSIM
                     SFAIFMGIYNFMFGSVGLSIRGYKKEFSYIVAITGVSTIILSLCLSYFFAEIGAAIAY
                     VFAEFILLILILRIYKVKRL"
BASE COUNT          413 a          217 c          164 g          329 t
ORIGIN      
        1 gattattacc cacgccaata cataaacaat gcctaaacta tggaaaagag gtgccataaa
       61 gacaaaacaa agagagttaa atttatgctc tttttctaac tcgataagag aatttatgac
      121 aaataaaaaa agaaatgtat tcctgatttg aacaatgtca ataggcattg gacacaacat
      181 gtaaagcagc aaaccaaatg ctgtatactt actgtattgt attaatcgta ggaatgtggt
      241 cccaaggaat aaacccagca agccaattat gacttcatgg gtaccgccta aatacttaac
      301 accataaatt aaccagcgat aaccaatctc ggcataaagt tcattgacat taaatatatc
      361 aacatacgct tcatagtctt gattctgcct attaataata aataaagtag taataataag
      421 cacaattacc aaattactta gcttgtttgc tctagcgtag ttgaatgcaa taatccataa
      481 acctaaaacc atcacaaata tgatgtaata cattctttcc ccgcaattta ttcagtctat
      541 agccatgatg attttgcact atcagttctt accgggaatt ataatcgttt cactttataa
      601 attctaagta tgagaataag taagataaac tcagcaaata cataagcaat tgcagcgcct
      661 atttcagcaa agaaataact caggcataat gataaaataa tagttgaaac acccgtaatg
      721 gccactatat aagaaaattc ttttttatac cctcttatgg acaatccaac cgaaccaaac
      781 ataaaattgt atatccccat aaatattgca aaagacatta ttgataaagg gatgaacgcg
      841 cttctaattt cagcgatact ttgaacctta tatcccaata tagtacccac cccccctaaa
      901 ctcaggacaa tgaaaaccat gaactttagc tgaatatttg tgcttgcata tagcaggctt
      961 ttatattctc caactttgta tggttctaat ttcaattcat gttgctttat caacctcgtg
     1021 aaatagtatt gatttaacgg ggttgctata gactgaatac atttaataat tttctctgcc
     1081 gtcgcgtaca gagacgttgc aacaggcgaa gcaagagtgc caa
//
LOCUS       DAZFDP010000454         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFDP010000454 DAZFDP010000000
VERSION     DAZFDP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHJ1G_005668"
     CDS             complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHJ1G_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4767277.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSY"
BASE COUNT          356 a          246 c          207 g          311 t
ORIGIN      
        1 ataagatgaa tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata
       61 atttttcgac cataccacgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt
      121 aataaattga tttgaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc
      181 agcattgaga acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat
      241 actgatgcca tttctttgta acgtagtaag aagctcgctg tagtacccca tattggtttg
      301 tggcaacacc cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg
      361 ggacttactg gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg
      421 aagcttcatt tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc
      481 cggtaagaac gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc
      541 tttgaacttg ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa
      601 tgagacttcc accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat
      661 ttcacgggta atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac
      721 accggtattt ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat
      781 tttactgacg gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact
      841 tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat
      901 aacgacctac gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg
      961 atatgtgcga ccactcacaa attaactttc aatactttcc atggtatcgt tacgcaaatt
     1021 aaataccttt aaatatcaac aagttaaagt ataaaaatca gcataaaccc tgatttttgt
     1081 caaaaaaaat gacggggata accatatttt atggcgataa
//
LOCUS       DAZFDP010000455         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFDP010000455 DAZFDP010000000
VERSION     DAZFDP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            433..1120
                     /locus_tag="ACHJ1G_005669"
                     /pseudo
     CDS             433..1120
                     /locus_tag="ACHJ1G_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          324 a          211 c          221 g          364 t
ORIGIN      
        1 tctacagcgt catcacctta ttcagtcgac cttcaaaata aacgtttaac agagacaatc
       61 accatttgga ttgtccgctg aggaggcgaa caatttccta gacagcttta tcatgtcagc
      121 ccacattgtt ggcgctggca atactaagga caaacgatgg ggaagtagct ttttagcgtt
      181 attttcggga gtaaaaaacg tggtatcttt ccgtatgtca gttgtatctt gctggtattc
      241 gtagtcgtga taaggcatac aacgatattt atcttgacgt tagtgaagaa taagttgttt
      301 tgtgatctat gttaatctgg gattacaatc cgggcttgat acatcgcatc acgactttta
      361 ttctcttcag aatagaatat gttactcccc gctttacagg ctcaggattt gaattacaaa
      421 ttatactttt aagtgaacaa taaccaaggg tatttcttat ttcctgaaac caggcttggc
      481 ccatttcggg atgagtttga aaaaactctt gacgcatacg atagtgatga aataaacgaa
      541 acaaattacg tcaacaaact caggcgtctt gcctgacatg ctcctgacat cattgatatt
      601 cacgctcatc tggcatatgc atttgcgctc tttatgctgc aattctggcg aatgttcatt
      661 tacagcgtca tcaggatgcc gttatactca ttgataaaat actaacatat aaccctgaga
      721 ataatcaggg aacacgctgg ttactggggg cggaattgtt acgtgctggc aagcatggca
      781 gggctctgta cgtattgaca ggacatgtca atgaatttcc cccctactgg tacgagcttg
      841 ctcttttaca ttttatgaat gaggcatata ttaatgctgc aacggcattt cgatatggat
      901 ttttaacaag tatatattgt tgaaatgctt tgttagaatc tttatccatt ccctagggct
      961 gtatagtatg atttttcagg tatttctgaa tactgccaaa tattattgca aaatattctc
     1021 taaattatga attaaatatc cataagcatt attgcctggg aactggacat ataattactc
     1081 ttctgtgcta aaggaacgtt ctgaaatgtt taagtatgca
//
LOCUS       DAZFDP010000456         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFDP010000456 DAZFDP010000000
VERSION     DAZFDP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..509
                     /locus_tag="ACHJ1G_005670"
     CDS             <1..509
                     /locus_tag="ACHJ1G_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4767278.1"
                     /translation="EFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQN
                     GKFRYCMPGDNLPAEPKAALVTESDGKAFPQPAGAALPVREAATQEEIKTESVAVTVQ
                     SQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKHRDIVRQITDS
                     SRRVVSEK"
     gene            525..947
                     /locus_tag="ACHJ1G_005671"
     CDS             525..947
                     /locus_tag="ACHJ1G_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4767279.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
     gene            1053..>1120
                     /locus_tag="ACHJ1G_005672"
     CDS             1053..>1120
                     /locus_tag="ACHJ1G_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767280.1"
                     /translation="MLNTQKAINAEKYNEWARKFSE"
BASE COUNT          318 a          234 c          323 g          245 t
ORIGIN      
        1 ttgagtttat cggtcaggat ggggctaaaa cgtgtgagga actggcgggt aagttcggtg
       61 tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac
      121 gcgttaatca gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc
      181 cgaaagccgc gctggtaacg gaaagtgatg gtaaggcctt tcctcagcca gcaggtgctg
      241 cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca
      301 cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc
      361 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
      421 tctgcgccgc gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt
      481 cttcccgccg tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta
      541 cacaggaaga gcgggaaaaa attaaagggc aggttgttga gctagtacgc cggagtgggc
      601 gcgagacgtt acggcaactg gaagccaaga caggtgcgac aagatatctg atgagcgttc
      661 ttgccagaga gctggttgcc agtggcgatg tatacaactc tggttacggg ttattcccat
      721 ctgaacaggc tcgtaaggac tggcaaaacg cccgcaaaaa actatcgagg gcaaaactga
      781 agaaaccggt tgtggttgat cctgacctta tctggtcatt accagacgga gaaatacgcc
      841 gctacgacag gcgtctgaac ataatctgta gcgagtgccg gaagagcgaa gctatgcagc
      901 gtgtactggc tttctatcag ggtaattttc agaaggtgct gttgtgagcc aaattaacaa
      961 tcggaacttc gtgaagagaa agcataatcc aaatctgaat aattaagttc agcactgtaa
     1021 ataaaattta atccttaact ggaggtatat ttatgttaaa tacacagaaa gccattaatg
     1081 cggaaaaata taacgagtgg gcaagaaaat tctctgagca
//
LOCUS       DAZFDP010000457         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFDP010000457 DAZFDP010000000
VERSION     DAZFDP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1001
                     /locus_tag="ACHJ1G_005673"
                     /pseudo
     CDS             1..1001
                     /locus_tag="ACHJ1G_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            998..>1118
                     /locus_tag="ACHJ1G_005674"
     CDS             998..>1118
                     /locus_tag="ACHJ1G_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4767281.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYI"
BASE COUNT          248 a          260 c          377 g          233 t
ORIGIN      
        1 tcgtcaggca cacgccagga gggcgcgtct gacggtaata aggccataag cgaaacagag
       61 cgtgatgcgg tgaaggcgat gttcaggggg atactgcgaa aatgacagag cgtgaactgt
      121 ccattatccg tgcgctgggt gaagaattct ccacggtgct ggcggattta cagcgcacat
      181 ttgaggggaa gatggcctcg caggcacaag cgtttgaaga gaaactgact tccctgtcgg
      241 cggtattaca gaagcatgtg acggtggatg aggtgcgtcc ggttctgcag gtgatggtgg
      301 atgacgctgt gggggccatt ccggtaccgc gtgatggtcg tgattatgat ccggatgtac
      361 tgcagcaggc ggtgaatgat gcggttgcga atattccggt accggcggac ggcaaaagta
      421 ttacccccga tgatgtgcgt ccgatgcttg aacagatggt gaaagaggcg gtaagccata
      481 ttcctgttcc gcgtgatggt cgtgactatg atcccgatgt tctgcagaag gctgttctgg
      541 aggcggtgaa tgccctgccg gctccgcagg acgggcgtga tgccacggct ctggaagtgc
      601 tccccgctat tgacgatcaa aaatcctttc cccggggcac gtatgccaca cacctgggag
      661 gattctggcg ggcgtatgaa aaaacgcacg gaatgcgggg atgggaatgc ctggttgacg
      721 gggtggcgga tattgacgtc agtatgacgg gggagcggtt gttctctgtg gtgatccggc
      781 acagcagtgg ccagcgtacg gaaaaaacat tttccctgcc ggtgatgctc taccgcggtg
      841 tgttcagagc cggtgaaacc taccaccccg gcgatacggt gacgtggggg ggctcgctgt
      901 ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca ggctggaccc
      961 tggctgcaaa acgtggacgg gatgcaggag gcggaaaatg acggcattac tgacactgga
     1021 agagatcaag gcacatctgc gtgttgacca tgacgcggat gatgacatgc tgatggacaa
     1081 ggttcgtcag gctaccgccg tgctgctggc ctacattc
//
LOCUS       DAZFDP010000458         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFDP010000458 DAZFDP010000000
VERSION     DAZFDP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..100)
                     /locus_tag="ACHJ1G_005675"
     CDS             complement(<1..100)
                     /locus_tag="ACHJ1G_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767282.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDI"
     gene            complement(84..>1114)
                     /locus_tag="ACHJ1G_005676"
     CDS             complement(84..>1114)
                     /locus_tag="ACHJ1G_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4767283.1"
                     /translation="PLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGL
                     LDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLA
                     LISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQ
                     GRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAAN
                     SITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGD
                     RQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQ
                     GSSDASNY"
BASE COUNT          264 a          324 c          222 g          304 t
ORIGIN      
        1 cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa caaagggtaa
       61 ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc ttgctttgct
      121 tttattggat tatctgcttt tataccatct aacccgaaga gttgcccagt gttatatttt
      181 tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc gacagtgtta
      241 tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc tgccgcctca
      301 tgatttttac ctatcacgtc agctattgtt gatccaaatg tcttagtcat tcgacacatc
      361 aagtaacaat gtctcattgc atcggcggcc ccattatgag ctccgccaac aatagcatcc
      421 atatcataac cgaactggta agcactgtta gtaatgctgt ttgcagcaat caatccaggt
      481 acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc atctaacccc
      541 attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca tccacctctc
      601 aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta ccggtgacgg
      661 ttatagtaca gccccgactc cttatcatac tgctgccccg gcagccggta cggctggtgc
      721 aggtgatgcg ggttctcctc attaagctgg ttgccccatt catcatattc cgcgctccac
      781 gccgtattgc cgtcttcact gataagcgcc agcggcaggc cccggtggtc acagtgataa
      841 agatgtattt ttcgctcagg caaataaacc ggctctatct gtgccgccag ccgctccacc
      901 gtcagcccgc actgcgcaag ccactggcgg ctttcactgc tcacgcagtt tgcccggatt
      961 tccccctcca gcctgtccag cagccccacc agttcagccg ggaacaccac accgtgaccg
     1021 tcttcgctcc cttcctgctg tatcttctcc gccaggctgc ggcactgcgc tttctcccgt
     1081 tcaccgttgt ctgtttcgat acggatgagc ggcg
//
LOCUS       DAZFDP010000459         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFDP010000459 DAZFDP010000000
VERSION     DAZFDP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..248
                     /locus_tag="ACHJ1G_005677"
     CDS             <1..248
                     /locus_tag="ACHJ1G_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767284.1"
                     /translation="RKTRDKTAPKYKALDMTELSLKVAIRTIDRHVGEGYAKEHPDLI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            245..550
                     /locus_tag="ACHJ1G_005678"
     CDS             245..550
                     /locus_tag="ACHJ1G_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767285.1"
                     /translation="MSEPKFGEKLYKHNGRITIQQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            560..970
                     /locus_tag="ACHJ1G_005679"
     CDS             560..970
                     /locus_tag="ACHJ1G_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4767286.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            983..>1098
                     /locus_tag="ACHJ1G_005680"
     CDS             983..>1098
                     /locus_tag="ACHJ1G_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4767287.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHH"
BASE COUNT          334 a          253 c          321 g          190 t
ORIGIN      
        1 acagaaaaac cagagataag acagcgccaa aatataaagc gttagacatg acagagcttt
       61 ccctaaaggt ggcaatcaga acgatagacc gacacgtagg ggaaggatac gcgaaggaac
      121 atcccgacct gataagcgca ttcatgacca cagcagcggc aaactttgcc acgctgacag
      181 aacgggagat tgccgaagcg gaacaggtaa caaccatcaa cgttaaaacc ggagaggtgg
      241 aatcatgagc gaaccaaaat ttggtgaaaa actttacaag cacaacggac ggatcacgat
      301 tcagcaaata agcgcagcta caccaggctg gtgggttgaa acagacgaag gatcatcacc
      361 cgtggcatca tgggcgcttt gtgtgcttag ttaccctgac cgcgatgtgt atcaggatat
      421 tctgcctgtg atatccactg acaaaggcat gaaacccgta gacatcaaaa acatgggttt
      481 tcagtgcgta atgctcactg aaaaaatgat ggaagagatg aaaaagaaca acgccggatc
      541 gcttcactga ggtggcaata tgacagcaca aattgcagct tacgggcgcc tggtggacga
      601 cccgctggta aaacagagca gcaagggcac accgatgact ctggcgcgta tggcggtatc
      661 gttgccatgc agccaggcac atgacggaca ggcgacgtta tggttatcgg ttatcgcatt
      721 tggcaaacag gccgatttcc tggctaaaca tcaaaaaggc gatgttgcca gcgtatccgg
      781 cacgatgcag gtaagccagt ggaccggaca gaacggggaa acacggcagg ggtatcaggt
      841 tattgcagac agcgtaatca gtgcccgtgc ggcacgtcct ggcgggaaca gacgcaaaac
      901 cacaggcaca cagggtaatc agccaccagc gggaggcgat gacccttacg gtgatgatat
      961 tccgttctga ggggtgggga cgatggtaca tgaccgcata gcggaagagc tggaggcgaa
     1021 aggcttttac cggagagcgt cggcgcgatg gggtgaagtc atgcagctgg tggagacaga
     1081 caaggaacgg catcacat
//
LOCUS       DAZFDP010000460         1088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFDP010000460 DAZFDP010000000
VERSION     DAZFDP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..749
                     /locus_tag="ACHJ1G_005681"
     CDS             <1..749
                     /locus_tag="ACHJ1G_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4767288.1"
                     /translation="FEGINLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHEL
                     EEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGK
                     NIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPLRIYPVFTNIINNAMYWVSLSN
                     NRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHH
                     KIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            751..>1088
                     /locus_tag="ACHJ1G_005682"
     CDS             751..>1088
                     /locus_tag="ACHJ1G_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO4767289.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLK"
BASE COUNT          361 a          172 c          204 g          351 t
ORIGIN      
        1 tatttgaagg tataaactta gattcagcat tctcattatc tgaagaagaa cgctcatatt
       61 ttgaagaaaa agctaaaagt ttaaacgcgc ttgcacagtt aggtattagt gttgagataa
      121 tatctcatga acttgaagaa atggattcta tggtaaccag aggactaaac tctcttccta
      181 cttctgtaaa agaacaccct ggtttttcat tggcgttaaa tgctcacaga tcgcttactc
      241 aacaaatacg tttcttatca cctttgaaaa tatcaggtta tcaatccagg cagagaataa
      301 ctggaaaaaa tatcatggat tatgtcctga agttctttgg ggagcgtttc gaacggcaac
      361 gaataactat tgaatttagt gaagagttta agcaaatcgc aataacagat ataccattaa
      421 ggatctatcc tgtttttact aatattatca acaatgcaat gtattgggtc agtctgtcaa
      481 ataataggct cataaagatt ggttttgtga attctttggt tatcatagca aattctggtc
      541 cggcaattga taccgatgat atcccgcgac tatttgaact attttatagc aaaagagcaa
      601 atggacatgg ggtaggtctg tatctatgtc gagaaaacct tgctgttgca catcataaaa
      661 tatggtattc agaacctgat gaaggcgata actatttaat aaaagatggc gctaattttg
      721 tgatccagtt caatggagtg gagttctaat atgacagtgg caaattataa ttctcttgtc
      781 cagaaaactt tctgcgaaaa tgcaattcgt tccgttgtca tgattgatga cgattttctg
      841 acgtattctg aatcaatcag ggcgttgaat aacgaagttg atttagacta caacaaaatt
      901 gactcatcta aacgagccgc tactcttgag agcttttttc aatctaaaaa tatgatttgt
      961 gatgttgaca atggttctgt taatttcgat gtggatcgga ttagaaaatc agatcttatt
     1021 attgtagatt atcatcttga taataatgca cctgataaaa cacttaaact attacaagat
     1081 ttgaaaga
//
LOCUS       DAZFDP010000461         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFDP010000461 DAZFDP010000000
VERSION     DAZFDP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..613
                     /locus_tag="ACHJ1G_005683"
     CDS             <1..613
                     /locus_tag="ACHJ1G_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO4767290.1"
                     /translation="RKMEFTYPGANKSHLQSSNSDVVAAAAISITATEMKTILPDDLT
                     PGKYNKIYLSGHGSAGLPLLKCGDEFLSPSDIVDRIVQHNLHEIDDIRLTSCNSANII
                     KNKDFSPDEIEKSANMNNGWLARALFGQKRSLAEHVYAEFERRGINVSISGYHGTGVF
                     YVPEHGKPTTHLRSTTVPATPEHTVRRSDYRATLGRTQPIDIE"
     gene            complement(838..>1077)
                     /gene="nleG7"
                     /locus_tag="ACHJ1G_005684"
     CDS             complement(838..>1077)
                     /gene="nleG7"
                     /locus_tag="ACHJ1G_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO4767291.1"
                     /translation="ITLCVPEKGVFVKNALNSNICTLYDKSAFMNLTREHLPHPLSRE
                     KIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
BASE COUNT          333 a          213 c          218 g          313 t
ORIGIN      
        1 aaggaaaatg gagtttacat atcctggagc aaataaaagc catctacaat caagtaatag
       61 cgatgttgtt gctgctgcag ctataagtat tacagcgaca gagatgaaaa ccatcctgcc
      121 agatgattta acaccaggaa aatacaacaa aatttatctg tctgggcatg gttctgctgg
      181 tctacctctt cttaagtgcg gagatgaatt tttatcaccg tcagatattg tcgaccgcat
      241 tgttcaacat aatcttcatg aaatagatga tatcagatta acatcctgta actcagccaa
      301 cataataaaa aacaaagact tctctcctga tgaaatagaa aaatccgcaa atatgaataa
      361 cggctggttg gccagggcat tatttggtca aaagaggtct ttagcagaac acgtctatgc
      421 cgagtttgaa cgtcgcggaa ttaacgtttc tatatcaggt taccatggca ctggcgtttt
      481 ttatgtacca gagcatggta aaccaacaac gcatctacgc tccacaactg tgcctgcaac
      541 acctgaacat actgtaagaa gaagcgacta cagagccact ttgggtagaa ctcaacccat
      601 agatattgag taattgagcc agcatataca cttgatggtt aggaaacaga atcattgttt
      661 taaacatttc ctggccatta atattgtcgg cgggagcata tccaggactg gccggcaaac
      721 cgggtacgcg atctgttgcc atggaaagtt gatctgagcg ctcagtaaat atcaatacgg
      781 ttctggcgag ccgcttacga tgcatgagaa tcactaatag tatgtaatta ttacatttta
      841 tttacaatgc tgtttctgtt ggtctgcatc cataattatg aaatgctgac ttatggtgtc
      901 aaaataacac atattccttt caataatcat ttcttttact atcttttccc tgctgagagg
      961 gtggggtaga tgttctcttg tgagattcat gaacgcagac ttatcataaa gagtgcatat
     1021 gttggagttc agtgcgttct tgacaaaaac ccctttttct ggaacgcata atgttat
//
LOCUS       DAZFDP010000462         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFDP010000462 DAZFDP010000000
VERSION     DAZFDP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..175)
                     /locus_tag="ACHJ1G_005685"
     CDS             complement(<1..175)
                     /locus_tag="ACHJ1G_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4767292.1"
                     /translation="MSIKHYDVIRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPS"
     gene            152..295
                     /locus_tag="ACHJ1G_005686"
                     /pseudo
     CDS             152..295
                     /locus_tag="ACHJ1G_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(490..657)
                     /locus_tag="ACHJ1G_005687"
     CDS             complement(490..657)
                     /locus_tag="ACHJ1G_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4767293.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(654..1077)
                     /locus_tag="ACHJ1G_005688"
                     /pseudo
     CDS             complement(654..1077)
                     /locus_tag="ACHJ1G_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          276 a          312 c          234 g          255 t
ORIGIN      
        1 atgaagggcc gacctgtggc tctccttcaa tagccaccgc ctgcatcagt gtgtacggcg
       61 taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt gtcagctttt
      121 ccgcaaggtc tgacggcgac gccgccctga taacatcgta atgtttaatc gacatcgaat
      181 ttctcccgtg tagaggaaca gagttaaaaa gccggaagcg gaatcaaatc acaggatgac
      241 catctgccag tggctggtcg taaaaaaaag gccgcgccat gcgcagccga aaataaaggg
      301 ataacgatga tagtttgaga aaaacagaaa taacactttt gtggcaaagc atggtgccgg
      361 gtgcctcccg gtgaattcag tatcagcacc tgaatccgcg attaccccat attccttctt
      421 gctgattgcc ccaccgcaca gggggattca ccatgcagaa gtgtttttaa taaacagcaa
      481 acaaaaaaat caagcattat gcaggctgtt tctttttatc accggccaca gcaataccat
      541 aatgccgcag accagcaccc catccgccag caccgacatg attctgctgg tgaaatccac
      601 catcaccacc agaaacagca ggagtgcagc cacagtcagg cgcagtttta ccgtcacagg
      661 taattctcca gacgaagacc cagaacaccg gcaatctctt ccagcacctt gcgctcttcc
      721 ggctcaattt cgccgtctgc ctccgcaatg gccaccgcca catccagcac atcttccgct
      781 tcacgcgtat cgtgtttcac atcctcgatc tcacgtaacg ccgcacgacg accagtttta
      841 aagttcgtat ccagctgacc gataatggtt gcgctaatcg cattaatttc tgacgtaaac
      901 gcgtacagcg caggctgatt acgcagtacc tgttcgatct tcgctttcta ggaagcctca
      961 cattcaccat ctgcataggc caccaggtag gcggcgttaa tcaccacctg tgccagatcg
     1021 cgtttttcaa actttttaat ttccgttgcc gctctgcggg ctttttttac caaaaat
//
LOCUS       DAZFDP010000463         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFDP010000463 DAZFDP010000000
VERSION     DAZFDP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /locus_tag="ACHJ1G_005689"
     CDS             <1..183
                     /locus_tag="ACHJ1G_005689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767294.1"
                     /translation="HKFIFFVEKINCLGEIILYDRDAAGHLAEVITQEDKSLYPFEKS
                     GRLMGIFSPGGTSQRI"
     gene            310..747
                     /locus_tag="ACHJ1G_005690"
     CDS             310..747
                     /locus_tag="ACHJ1G_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO4767295.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            767..1075
                     /locus_tag="ACHJ1G_005691"
                     /pseudo
     CDS             767..1075
                     /locus_tag="ACHJ1G_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          318 a          210 c          265 g          282 t
ORIGIN      
        1 cataaattca tcttctttgt tgagaagatt aactgcctag gtgaaattat cctctatgac
       61 cgggacgctg caggccacct ggcagaagtc atcacgcagg aagacaagtc gctgtatccc
      121 tttgaaaaat ccggcaggct aatgggcatc ttcagcccag gcggcacatc acagcgaatt
      181 tgaaggcaaa tgcgaaaatt agtgatcttg aagttcatac agtattggta aaaacaggcg
      241 aagcttttcc aatgtgtaag aattttcaaa tttcaatatt tggcgctgtt gttacaagcg
      301 ggtgattata tgtgtgaatt aattaatgaa gcatcaaaat atgggaaagt caatgaaaga
      361 aagatggaca ttgatttgtg ctgtaaaaaa atagaggaag aaggatattc tgttttcgtt
      421 gcgttctatg attttataaa aaaatatggc ggtttatatg gctttcattc agcttacaaa
      481 gacaccacta taaatgaggc tttttttata gatccagaaa gggcctctaa tcgcattttt
      541 atagaaagag tcaatgatta tattgagaga gctggttaca atatgattcc tgttggcgag
      601 tgcagtaatg gtcatatcac attattatat ggggaaaata atgtgatata tggcggcttg
      661 gatgattatc tttggaagta tggggataat ttatcttcat caattgatat attgattaat
      721 ggcaaggagg cagttgaaat tccgtaaggc caacttgata tgacacctgg cacacaaccc
      781 cgacgaccgg atgtatatgc tgggcgggat gagtgacacc gccagcaacc gcatcacgtt
      841 tgagcgcgac agccagtacc ggatcacgtg tgtcagtcac accgacggga tccggcttaa
      901 actgacgtac catgccagcg gctacctgaa agccattcac cgcacggata acggcataca
      961 gacgctggcg acctacgaac aggatgcgcg ggggcggctg accgaagcgg atgcgcggct
     1021 ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct ggtcc
//
LOCUS       DAZFDP010000464         1074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFDP010000464 DAZFDP010000000
VERSION     DAZFDP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1074
                     /locus_tag="ACHJ1G_005692"
     CDS             <1..>1074
                     /locus_tag="ACHJ1G_005692"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO4767296.1"
                     /translation="EDLGINGIYFCPIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLI
                     EEAHKRGIKIMLDAVFNHLGADSPIWLDVVRNGANSRYADWFWIHKFPVYPDTPKSEW
                     DFKNFNYETFGNVIEMPKLNTENEECREYLLSIVRYWTQNFDIDGWRLDVANEVDHHF
                     WRDFRKVIKDIKPECYILGEIWHEGTPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQ
                     EFMTSVTRSYLCYPKNITEVMFNLLDSHDTARILSVCLNDKRKVKLAYLFMLTQAGSP
                     CIYYGSEIGIDGFKSMTLENNRKCMIWDENKQDLELRQFIRWLIRLRKKHPQWCVASI
                     QWKDVEHPTVIAYQRDNITFFLN"
BASE COUNT          332 a          173 c          215 g          354 t
ORIGIN      
        1 tggaagatct gggtattaat gggatttatt tttgtcctat ttttacctca gcttccaacc
       61 ataaatatga caccatcgat cattttagtg ttgatccaca tttaggtggg aatattgctt
      121 tccatacttt gattgaagaa gcgcataagc gtggaataaa aatcatgttg gatgcagtat
      181 tcaatcattt aggcgctgac tctccaatat ggcttgacgt tgtacgcaat ggtgccaatt
      241 cacgttacgc cgattggttc tggattcata aatttcctgt ttatcccgat actccaaaaa
      301 gtgaatggga ttttaaaaac tttaactatg aaacctttgg caatgtaatt gaaatgccca
      361 agctaaatac tgagaatgaa gagtgccgtg aatacttact tagtatagtt cgctattgga
      421 cgcaaaattt tgatattgat ggttggcgtc tggatgttgc gaatgaagtg gatcatcatt
      481 tctggcgaga tttcagaaag gtgattaaag atatcaaacc tgaatgttat attttgggtg
      541 agatatggca tgagggcacc ccctggttgc gtggtgatca gtttgattca ttaatgaatt
      601 atcccttaac atatgggatt attgattatt ttgcgttaca ggatacaaca aaacaagaat
      661 ttatgacatc cgttacacgt tcttatcttt gttatcctaa aaatataact gaagttatgt
      721 ttaatcttct ggatagtcat gacaccgcgc gtattttaag cgtttgtttg aatgataaac
      781 gaaaagttaa actcgcctat ttatttatgt taactcaagc gggctctcct tgtatctact
      841 acggtagcga aataggaatt gatggtttta aatcaatgac gcttgagaat aatagaaaat
      901 gtatgatatg ggatgaaaac aaacaagatc ttgaattacg gcaatttata cgttggttaa
      961 tacgtctacg taaaaaacat ccacaatggt gtgtggcatc aatacaatgg aaagatgttg
     1021 aacatcctac ggtgatcgct tatcaacgcg ataacatcac tttcttctta aata
//
LOCUS       DAZFDP010000465         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFDP010000465 DAZFDP010000000
VERSION     DAZFDP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(82..189)
                     /locus_tag="ACHJ1G_005693"
     CDS             complement(82..189)
                     /locus_tag="ACHJ1G_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4767297.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            complement(618..725)
                     /locus_tag="ACHJ1G_005694"
     CDS             complement(618..725)
                     /locus_tag="ACHJ1G_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4767298.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT          235 a          291 c          257 g          288 t
ORIGIN      
        1 aaaaaaggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg ccatcacggc
       61 agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa tcgctgcggt
      121 aataattccc gccaggatcg atgctgccag gtcgtgccag aaaatcatgg caaactgcgc
      181 gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct tgaattgttg
      241 atggttcaag attagccccc gttctgttgt caggttttac ctctcaacgt gcgggggttt
      301 tctctttcca gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg
      361 ataacgccgg cttggtgtgg atactacgac tcaattcatc ttcacttcat ccctgaaatg
      421 tttgcaatga agagtgcatt ccggtttttc aacagctgtt acagtcgttt catgagtgct
      481 ctggatgatg cttccagctc gggttgccaa tatttacttg tggaagagat aaagacaaaa
      541 atggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
      601 tgacgcccgt tttcacctta cttccggtta cgccaccagc tgacaatcgc tgcggtaata
      661 attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc
      721 gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa ttgttctggt
      781 tcaagattag cccccgttct gttgtcaggt taaacctctc aacgtgcggg ggttttctct
      841 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
      901 gccggcttga tgtggatact acgtctcaat tcatcttcac ttcatccctg aaatgtttgc
      961 aatgaagagt gcattccggt ttttcaacag ctgttacagt cgtttcatga gggctctgga
     1021 tgatgcttcc agctcgggtt gccaatgttt actggcggaa gagataaaga c
//
LOCUS       DAZFDP010000466         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFDP010000466 DAZFDP010000000
VERSION     DAZFDP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..87
                     /locus_tag="ACHJ1G_005695"
                     /pseudo
     CDS             1..87
                     /locus_tag="ACHJ1G_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
     gene            89..382
                     /locus_tag="ACHJ1G_005696"
     CDS             89..382
                     /locus_tag="ACHJ1G_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO4767299.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(443..1071)
                     /locus_tag="ACHJ1G_005697"
                     /pseudo
     CDS             complement(443..1071)
                     /locus_tag="ACHJ1G_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          341 a          198 c          176 g          356 t
ORIGIN      
        1 ataaaaggta ctgtttagtt gtttatagca ctctgtgaat gggataaaca ttattttaaa
       61 ctccaatctt gcgtaagaga aagttgatat gtcattagta ggatataata gtaacatcat
      121 aataaccatc gcgctgatat cgttgctctc tgcatgcacc agagaaaatt cattaaagga
      181 gaatcaaaat aaaagaagtg gcgaatccca agcgatagta gatgcaagac agttctttag
      241 cgaacacccg gaatatatat catcagcaga actggagcaa aagctatatc gcgaatttgc
      301 acaggttact acagttccag cttataaaga gatgagtatg tatcaattgc ttatcatttc
      361 acaagatcgt ttaactcatt aaataaggat ctacacgctt tttgcgtgta gatccttata
      421 aatttctgac tacgacagat gtttaatttt ttaaattatc gtctttattc tcgatttttg
      481 tgtcttcacc ctcaatgaat ttatcttctg aagagagctg ttcatcttga acaggttgcc
      541 cagcattctc cacatcttca agccacagca gaagacgtaa tatttcatct atattttcga
      601 agctaacata atcgtagcga cgatgggtag cataattttt tcgtgctaat tttttatctg
      661 tgataattgg tattccgatt tcctttgcat atttccttac gcctaaggcc acctcattag
      721 ttactcttac agaaatcaat ggaataggtg aaagatgtgg cttaaagtat atccctattg
      781 caatgtgagt agggttggaa atcatcaaac gactattact gacatcagac ttcaattgct
      841 cagaaagaat ttcctgatgc gctctctgcg tttagactta atttctggat ttccttcttg
      901 ctccttgtat tctcttttaa cttcttgttt atccattttc atatctttca taaataaaaa
      961 atattcagcg ataaaatcaa aaattaagac aattatcata ctgccgagac aatacagtat
     1021 gagaaggaat agcatctctc cccaatctga taataaagat atgatatctc c
//
LOCUS       DAZFDP010000467         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFDP010000467 DAZFDP010000000
VERSION     DAZFDP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1071
                     /locus_tag="ACHJ1G_005698"
                     /pseudo
     CDS             1..1071
                     /locus_tag="ACHJ1G_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT          378 a          201 c          199 g          293 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa
      961 cattattatt aataataccc gaaaaattat cccccctggt aaagtattta cgcttagagg
     1021 cggaactcta aatatcaata ttcctgggcg tagcgctctt cttttaggga a
//
LOCUS       DAZFDP010000468         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFDP010000468 DAZFDP010000000
VERSION     DAZFDP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1049
                     /locus_tag="ACHJ1G_005699"
     CDS             <1..>1049
                     /locus_tag="ACHJ1G_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4767300.1"
                     /translation="LELLAEARGDRAVFLTVTCPSKYHATTENGHPNPKWNGATMRDS
                     SDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEIDSIVAITR
                     DIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDSRAVDGIDPKTGKP
                     RVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMARNPEGPQ
                     RLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDYGEQSVQI
                     YGIWSPLIGESSRVCTHPDNWKLVRRKPEPEDNAHENGFDLQGGPAASWTRGNNCPRV
                     QETDNNGTKQPEERPA"
BASE COUNT          259 a          313 c          291 g          186 t
ORIGIN      
        1 ctggaacttc tggccgaagc ccgtggcgac agagcggtgt ttctgactgt cacctgcccg
       61 tcaaaatacc acgccacaac agagaacggt catccgaatc ccaaatggaa cggggccacc
      121 atgcgcgact ccagcgatta cctggttaac acgttttttg cggcagtccg caaaaaactg
      181 aaccgcgacg gtctgcgctg gtatggcatc cgcacggtgg agcctcacca tgacggcacc
      241 gtgcactggc atatgatggt ctttgctcat ccggaagaaa tcgacagcat cgtggccatc
      301 acccgcgata ttgccattca ggaagaccgc cacgagctgg gcaatgatat tactccgcgc
      361 tttaaggtgg agtatgtcga cggctcaaaa ggcacaccaa ccagctatat cgcgacctac
      421 atcggaaaaa acctggacag ccgcgccgtg gatggcatcg acccgaaaac gggcaagcca
      481 cgcgttgacc acgaaaccgg aaaatcaatg gccgagagcg tggaacgcgc catcggctgg
      541 gcgcgccttc accgggttcg ccagttccag ttctttggta tcccctcccg tcaggtgtgg
      601 cgtgaactgc gccgccttgc cagccagatg gcacgcaacc cggaaggccc gcaacggctg
      661 aaggatgacg caatggatgc ggtactcgct gccgctgatg ccggatgttt tgccacctac
      721 attgaaaaac agggtggcgt gcttgttcca cgcaaggact acctgattcg caccgcctac
      781 gacctcgcag atgagctgaa cgattacggc gaacagagcg tacagattta cgggatctgg
      841 tcaccactca tcggggaatc ctcccgtgtg tgcacgcatc cggataactg gaagctggta
      901 agacgtaaac cagaaccgga agacaacgcc cacgaaaatg gttttgacct tcagggcggc
      961 cctgccgcct cttggactcg tggcaataac tgtccccgtg tacaggaaac ggacaacaac
     1021 gggacaaaac agccggaaga acggccagc
//
LOCUS       DAZFDP010000469         1043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFDP010000469 DAZFDP010000000
VERSION     DAZFDP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..190
                     /locus_tag="ACHJ1G_005700"
     CDS             <1..190
                     /locus_tag="ACHJ1G_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4767301.1"
                     /translation="AQLRLGPADILESDENGIIPEQDRVITQVVILDTDKKLIQCVVR
                     PLQILRADGTWENIGGMK"
     gene            296..>1043
                     /gene="nleH"
                     /locus_tag="ACHJ1G_005701"
     CDS             296..>1043
                     /gene="nleH"
                     /locus_tag="ACHJ1G_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO4767302.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDR"
BASE COUNT          307 a          176 c          274 g          286 t
ORIGIN      
        1 agcacaactg cgactggggc cggcagacat cctggagtca gatgagaatg gcattattcc
       61 ggagcaggac agggtaatca cacaggtggt gatactggat acagataaaa agctgataca
      121 gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg
      181 gatgaagtaa cccgacagct tcacaaaacc ggagtccggc tccggttttt gttgtcatgt
      241 catggtgatg tttgttaaga aagtaaagat tgattcattt tgaaggttga aatgtatgct
      301 atcaccatct tctgtaaatt tggggtgttc atggaattct ttaaccagaa acctgacttc
      361 tcctgacagt cgtattttat cctctgtaag ggatgcagct gctagctcgg ataatggggc
      421 gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc actgataata agttttgcgt
      481 tacaagagaa agtcatagtg gttgctttac taatctgttg cacaggctgg gatggcctaa
      541 gggagagatt agcaggaaaa ttgaggtgat gctgaattca tcgccagtga atagggctat
      601 ggaaagaggt gctgttcatt cgaatagacc tgatttacct cctgttgatt atgcaccgcc
      661 agagttaccg agtgtggact ataacagctt gcctgtgcct ggtaatgtta ttggcaaagg
      721 gggtaacgct gtagtatatg aagatgctga ggatgcaaca aaagtcctga agatgtttac
      781 tacatctcaa agcaatgaag aggtaacaaa cgaagttcgt tgcttcaacc aatattatgg
      841 tgccgggagt gcagaaaaaa tatatggcga taatggcgat attattggta ttagaatgga
      901 taaaataaat ggagaatcgc ttttaaatat ttcgtccttg ccggcacagg ctgagcatgc
      961 catttatgat atgtttgaca ggcttgagca aaaaggaatt ctttttatcg atacaacaga
     1021 gacaaatgtc ttatatgatc gca
//
LOCUS       DAZFDP010000470         1035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFDP010000470 DAZFDP010000000
VERSION     DAZFDP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            848..>1035
                     /locus_tag="ACHJ1G_005702"
     CDS             848..>1035
                     /locus_tag="ACHJ1G_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767303.1"
                     /translation="MQNLILKNGNEELVITDETFNATAYMLGITPTELKKHCDKLFKS
                     ADEQKPTMQDLIKAKAEQ"
BASE COUNT          321 a          209 c          185 g          320 t
ORIGIN      
        1 aacagaaatg ttagaataat aaatgtgggt ttttgaatcg aaaatatgtg ttagctggcg
       61 ttaatacttc tcccgattct aatcctacac gaaagcgggg tagacctcaa gggggcgcgc
      121 cctatctaac ctatcccgct ttccctttac agtgattttt tagaaattgt ttccctcgtt
      181 aatagtttca ttttattttc cttattgctt tatcaaaatt gtgtcttaag aatgcagggg
      241 ctacggattt ggcccctcat ctttttaacc aactgcgaag catgaaacac tttctaaaaa
      301 cactgtttac agtgtctata aaccccgtat aaacaaataa cggtgtatat ctgtttcttg
      361 acaacagttt tctactaagc ttaaagctga atatgttttt gtttgttggt gtttattaag
      421 gtgccaatct tagaacgcga aagcgtggat gatccccgaa atcatctcgg caccgcctcc
      481 tattaaaatg agaatccaaa aggtttgaaa gttaataggt gattcctgaa agtctgatcg
      541 atgcatccac attgtgaaag acagcgatta gccgtcgcac gaataacagg tttatagatt
      601 cctctccaaa gggggcgacc tcctaagcgc ctactccctg taagcctgtt attcacccct
      661 caagggtata taacaataag caatcttatt cttatatgtc ctcctcttta agttcatata
      721 aactgcgatt tagtaacact ctataacatc cttcaaatgt tgtattcata aagccggaag
      781 ctttcccctt ccggttttat ttttatcagc actaaacaag actgttagga ataaaggtaa
      841 agcaataatg caaaatctca ttctaaagaa tggtaacgaa gaattggtta tcactgatga
      901 aacattcaat gctacggctt atatgctggg gatcactccc acagaattga aaaaacattg
      961 tgataagctt tttaagtctg ctgatgaaca gaaacctact atgcaggatc tgattaaagc
     1021 aaaagcagaa caaat
//
LOCUS       DAZFDP010000471         1032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFDP010000471 DAZFDP010000000
VERSION     DAZFDP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..543
                     /gene="nleB"
                     /locus_tag="ACHJ1G_005703"
     CDS             <1..543
                     /gene="nleB"
                     /locus_tag="ACHJ1G_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO4767304.1"
                     /translation="RDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAM
                     LKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKS
                     LENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDF
                     IEFKHENIIPNTSMYTSSSW"
     gene            592..>1032
                     /gene="nleE"
                     /locus_tag="ACHJ1G_005704"
     CDS             592..>1032
                     /gene="nleE"
                     /locus_tag="ACHJ1G_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO4767305.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIV"
BASE COUNT          337 a          157 c          214 g          324 t
ORIGIN      
        1 agggataatt atctgttagg cttattaaga gaagagttaa aaaatatccc agaaggtaag
       61 gactctttga ttgagtcata tgcagaaaaa agagaacata cttggtttga ttttttcagg
      121 aatttggcca tgttgaaggc tggaactttg tttacagaga cgggaaaaac tggatgccat
      181 aacatatcgc cctgtagcgg atgtatatat cttgatgccg acatgattat taccgataaa
      241 ttaggagtcc tgtatgctcc tgatggtatc gctgtgcatg tagattgtaa tgatgagata
      301 aaaagtcttg aaaatggtgc gatagttgtc aatcgtagta atcatccagc attacttgca
      361 ggcctcgata ttatgaagag taaagttgac gctcatccat attatgatgg tctaggaaag
      421 ggtatcaagc ggcattttaa ctattcatcg ttacacgatt ataatgcttt ttgtgatttt
      481 attgaattta agcatgaaaa tattataccg aataccagta tgtataccag cagttcatgg
      541 taagtcttcc ggttgtggtt gaaaaatatc gttctttagg tatagattca gatgattaat
      601 cctgttacta atactcaggg cgtgtcccct ataaatacta aatatgctga acatgtggtg
      661 aaaaatattt acccggaaat taaacatgat tactttaatg aatcacccaa tatatatgat
      721 aagaagtata tatccggtat aaccagagga gtagctgaac taaaacagga agaatttgtt
      781 aacgagaaag ccagacggtt ttcttatatg aagactatgt attctgtatg tccagaagcg
      841 tttgaaccta tttccagaaa tgaagccagt acaccggaag gaagctggct aacagttata
      901 tccggaaaac gcccaatggg gcagttttct gtagatagtt tatacaatcc tgatttacat
      961 gcattatgtg agcttccgga catttgttgt aagatcttcc ctaaagaaaa taatgatttt
     1021 ttatacatag tt
//
LOCUS       DAZFDP010000472         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFDP010000472 DAZFDP010000000
VERSION     DAZFDP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHJ1G_005705"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHJ1G_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO4767306.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>1031)
                     /locus_tag="ACHJ1G_005706"
     CDS             complement(371..>1031)
                     /locus_tag="ACHJ1G_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4767307.1"
                     /translation="EENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYL
                     CVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKG
                     KFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGV
                     M"
BASE COUNT          318 a          241 c          167 g          305 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      961 ggccggactc ctcatcatac tgctgccccg gcagccggat aagctgctgc agctgatgcg
     1021 ggttctcttc a
//
LOCUS       DAZFDP010000473         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFDP010000473 DAZFDP010000000
VERSION     DAZFDP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..193
                     /locus_tag="ACHJ1G_005707"
     CDS             <1..193
                     /locus_tag="ACHJ1G_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767308.1"
                     /translation="EYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQD
                     ISTRDEGGDGKMVVIGRQA"
     gene            432..>1024
                     /locus_tag="ACHJ1G_005708"
     CDS             432..>1024
                     /locus_tag="ACHJ1G_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4767309.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNRTL"
BASE COUNT          268 a          248 c          308 g          200 t
ORIGIN      
        1 ggaatacagg gcaacggata acggcagaca gaacacgtac ttttcctcgc tggataacat
       61 gattgcccag gggaacccga tgccggtgcc ttacggtgaa atgctggttg gctcccggcg
      121 aatctcccag gacatcagta cccgtgatga aggcggtgac gggaagatgg tggttatcgg
      181 gcggcaggca taaaagcgaa aaaatcccgc agtgctcacg gacaggaact gcgggagcgt
      241 tacgaagatt gagtgtaagg aattattctt atgtcacgac aaaaaacatt aacgcagaga
      301 aattattagc gccacagtca gtttgtgaaa atgtgaagat attcagaatt tttattcagt
      361 catgatacag gcatcctccg ggatgcctgt tgtttttgtg cgtaacagtt atcacagtaa
      421 agggtgagac aatgggcaaa ggtggcggca aggcgcacac gccggtagag gcaaaggaca
      481 atcttaagtc cacgcagatg atgagcgtga ttgatgccat tggtgaaggg ccgattgaag
      541 gcccggtgaa ggggctgcag agtatcctgg tgaacaaaac cccgctgacg gacacggacg
      601 gcaatcctgt gatacacggt gtgaccgcgg tctggcgcgc cggggagcag gagcagacac
      661 caccggaagg ctttgagtcc tccggagctg aaaccggact gggcgtggaa gtgacgaagg
      721 caaagccggt aacgcgcacc attacatccg cgaacattga ccgcctgcgg gtcaccttcg
      781 gggtgcagtc actgttggag accacctcaa agggcgaccg taatccctct tctgtccgac
      841 tgctgattca gttgcagcgt aacggtaact gggtgacgga aaaggatgtc accattaacg
      901 gcaagaccac ctcgcagttt ctggcgtcgg tgattctgga taatctgcct ccccgcccct
      961 ttaacatccg gatggtcagg gagacggcgg acagcacctc ggaccagctg cagaacagaa
     1021 cgct
//
LOCUS       DAZFDP010000474         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFDP010000474 DAZFDP010000000
VERSION     DAZFDP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1020
                     /locus_tag="ACHJ1G_005709"
     CDS             <1..>1020
                     /locus_tag="ACHJ1G_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4767310.1"
                     /translation="GTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGA
                     STAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDG
                     QLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTKTLS
                     GVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQF
                     RGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNAL
                     NSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAH
                     KTPSMS"
BASE COUNT          211 a          245 c          359 g          205 t
ORIGIN      
        1 ggtaccgctt tttcggataa tgcggccctg tttgcccgtt cggctgcctc gatgcgtgaa
       61 tatggttaca gtgctgatga tgtgctgaag gtgacggagg ccatttccac ggggctgaaa
      121 atctccggtg ccagtacggc tgaggcgggt tcggtgatca cccagttcag ccaggcgctg
      181 gcacagggtg tgttgcgcgg tgaggaattt aattcggtca atgaaagtgg tgaccggatc
      241 attcgtgcac tggctgcagg catgggcgtg gcccgtaaag atctgaaggc gatggcggac
      301 gatggtcaac tgacggcgga taaagtcgtt cctgcgttaa tcagccagct gggggtattg
      361 cgtgatgaat atgccgccat gccagaaacg gtttccggta gtatcacgaa ggtggaaaac
      421 gcctttatgg cctgggtggg cggagtgaat gaggccagcg gggtgacaaa aacgctctcc
      481 ggcgtgctga acggtgttgc cggacagatt gataatgtgg caacagctgt gggtgcgctg
      541 gttgccgtcg gggttgcccg gtactttggc aatatggcct ccggagcgat gtctgccacg
      601 gcaggacttg tgacggctgc acgtaatgaa gttgcactgg cggaagcaca gttcagggga
      661 acgcagattg ccacagcgcg ggcaagggca gccgtgtacc gtgctcagca ggccgtggcg
      721 gcagcccgcg ggacggagat gcagattgct gcagaggccc gtctggcggc cacacaggaa
      781 cgcctgaaca gaaatattgc tgccagaagc gccgcccaga atgcgctgaa cagtacaacg
      841 gcggtgggct cacgtctgat gagcggtgcg ctggggctgg ttggtggcgt acccggactg
      901 gtgatgctgg gggctgcagc atggtacacg ctgtaccaga atcaggagca ggccagggag
      961 tctgcgcgcc agtatgcact gacgatagat gaaatcgcgc ataaaacgcc gtcaatgtct
//
LOCUS       DAZFDP010000475         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFDP010000475 DAZFDP010000000
VERSION     DAZFDP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..543)
                     /locus_tag="ACHJ1G_005710"
     CDS             complement(<1..543)
                     /locus_tag="ACHJ1G_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767311.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTP"
     gene            complement(695..>1018)
                     /locus_tag="ACHJ1G_005711"
     CDS             complement(695..>1018)
                     /locus_tag="ACHJ1G_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4767312.1"
                     /translation="WRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCY
                     DNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFE
                     NKNLA"
BASE COUNT          274 a          244 c          177 g          323 t
ORIGIN      
        1 aggcgtttta atttcactgc gcaacgcatc taagtttgta agatcactgt ctataatttt
       61 aagatagttt aaatctaacg aaccatcagt tttatcaagc ttagttttca gccttttaac
      121 agcctccaag gaagcatcaa gaactggtgt ctgattcttc aaagcttctg aaaaactttt
      181 ttgtgttgat tttcgagctt gttgttgagc agattttctt aactcttttt cacgcttaac
      241 ttgttctaaa agctctttac ggtcatcaga acgtgaacca aaaaatctat cagcaagttc
      301 agttaacaaa ttagatataa tagttttcag ttctcttgca gcctggtttc ttatgaatcc
      361 ctctctcccc gacttatctt tcagctcttt attactggat tgagtaattc caatataacc
      421 aaaaatcctt ctattagacc aataatatcg ccctgcattc catgaacgtc tttcttctat
      481 ctggaagaaa tcattatcta ctcgaccata aggtaatact ctcaagctat ccctaaaaat
      541 cattagtcct gcatactttt tggccttaag atcaaagtgg gaatgttcac gttcagtatg
      601 tgatgtattt tgtgaaagga attcaaacgt tcctatctga agctcaaatg ggccgacccc
      661 tgcgtgatcc tacccacgta atatggacac aggcctaagc gaggttcttg ttttcaaatt
      721 gttccggact gaggccgcca caccaactgt gccgccgcca ccgattgtaa tcacattcga
      781 tataattaaa caccgttgcc cgcattattt cccggctgat aaagtgttct ccatggatac
      841 attccacttt cagcgaatga aagaagcttt ccacgcaggc attatcgtag cagcaacctt
      901 ttgcgctcat acttccacgc agattatgcc gcttcagttg cgcctgataa tctgctgaac
      961 agtactggcc tccacggtcc gtgtgaacga taacgttccg gggcctctta cgccgcca
//
LOCUS       DAZFDP010000476         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFDP010000476 DAZFDP010000000
VERSION     DAZFDP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..506
                     /locus_tag="ACHJ1G_005712"
     CDS             144..506
                     /locus_tag="ACHJ1G_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767313.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYVSGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            525..>1004
                     /locus_tag="ACHJ1G_005713"
     CDS             525..>1004
                     /locus_tag="ACHJ1G_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4767314.1"
                     /translation="MILGNDYVDLIPLFQTHSTRNFLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     "
BASE COUNT          299 a          193 c          205 g          307 t
ORIGIN      
        1 caaattaccg aaatggtagc taaatacgct gggtgttctg tagaagaagt agaaagatgt
       61 gttgaaaaat atttttctcc ttcttctccg tctacacctt cccttaatga attaatcaaa
      121 ttaaaagcta aggagattac taaatgatta ttgatttaga cactctgttt ccagttcgaa
      181 agactttcac agatattgta agttattgta ctgatccgtt tgcctctgta gaaagcaagg
      241 tttttgcttc tcttcctgct gatgttgaat gcggcgactt gatcaccagc actggcgcta
      301 aatatgtatc aggagatgat atctatgttg ttatgagtga atttgtaacg gctggagaaa
      361 acaaacctgt agatgtttta cgtagcaatg ctgggcttgt atgcatcaaa gctgatgcgc
      421 tgaatgctgt aagtgaagca gcaaaaacag cattaattaa aaaaggcttc cagcttgaag
      481 ggttccattc tgttttcact tcttaataaa aggaataata ataaatgatt ctaggcaatg
      541 attacgttga tttgatccct ctgttccaga cacacagcac gcgtaacttc ctgctctcta
      601 ctcttgattt tacggattct gtaggggtta catctcataa agtagctgtt tcccagcttg
      661 tcgaaagcaa cgaatctctc tttaacaaag agacaagccg ttttagttct gaacacaatg
      721 ttactaagcg agaacaaggt aaagaatggc tgatcgagat cccttacttc ctgcgtgaag
      781 atgtgatccg tccttctgat gttcaaggca aacgtaaacc tggcactgat ttccaggaaa
      841 cactcactga tatttatgcg gaatatatcg ctaaacacca cgtagcgtat cagcgtacaa
      901 aagagagcgt attggctgct tctttgttct cgggcaaaac ttatacaccg aaaactgatg
      961 atgttcttat cgaatggggc aagctgttta acgtatcagc tatg
//
LOCUS       DAZFDP010000477         1003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFDP010000477 DAZFDP010000000
VERSION     DAZFDP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..227)
                     /locus_tag="ACHJ1G_005714"
     CDS             complement(<1..227)
                     /locus_tag="ACHJ1G_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4767315.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTT"
     gene            complement(519..743)
                     /locus_tag="ACHJ1G_005715"
     CDS             complement(519..743)
                     /locus_tag="ACHJ1G_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767316.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            complement(784..912)
                     /locus_tag="ACHJ1G_005716"
     CDS             complement(784..912)
                     /locus_tag="ACHJ1G_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4767317.1"
                     /translation="MVLVMKYGAITRTANHTAGEIRLFLHQLKRYGALPGFRHGLA"
     gene            complement(869..>1003)
                     /locus_tag="ACHJ1G_005717"
     CDS             complement(869..>1003)
                     /locus_tag="ACHJ1G_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4767318.1"
                     /translation="CRKAQRPPEPPDNYGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS
                     "
BASE COUNT          236 a          252 c          219 g          296 t
ORIGIN      
        1 tcagtggtct gactgctttt acctggcttg ctgcgctctt cgtctgcaat ggcttcaagg
       61 cgggaaatgt ctttatcgag ggattcagct ttagcgcgta attcgtcaaa ttttgcgccc
      121 tcagcatcgt taagactgcg gttttctttt tccgcgttct ccagcatgtc gcgcatctga
      181 tttttaatgg cggttttctg ctggcgtaat tcgattattc tcggcataaa aaaagtcctg
      241 gggttaagta aggaactcca ggacgcggca aacactcaac cgtttttcat aaggaaatca
      301 gtaatcgcac cgatcgtttt cccgcctggt aatgaatatt ggcgagcaca ttaacaggcg
      361 ggaaagtggc cccagcgtcc tggcaccacg ggcgagaata ctcatgattc agttcgggta
      421 aaatatgccg atcctgtcag tgaacaacgt ggaacaacca cgaacaaata atttacaaaa
      481 aatgacaaaa agccggattg ctccggctgt atgcgtgctc agtccctgat ccctttcaga
      541 aattctatca atgcatctat ctgttcagga tttaccgcca gcatttcacc ggatagagca
      601 catctaacaa aaccatgctg atccttttca atcagtgcgc ccgtctccag gaatgcgcgg
      661 taatcattga tgctcatcgt ctccatgttg tcagcatggt atttatcacg ctgttttaat
      721 atctcatcaa atttcatcgg cattgttttt tcctctgttg tgtctgtttg tttcagatag
      781 taactatgcc agaccgtgac gaaaacccgg taatgcgcca taccgtttca actggtgcaa
      841 aaaaagccgg atttctccgg ctgtgtgatt agctgtcctg gtaattgcgc catatttcat
      901 caccagcacc atctatcccc atttcggcat aagtgcgatc gactgccttt ttcaggtctc
      961 cgtaattatc cggtggctcc ggtggcctct gtgccttcct gca
//
LOCUS       DAZFDP010000478          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFDP010000478 DAZFDP010000000
VERSION     DAZFDP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..142
                     /locus_tag="ACHJ1G_005718"
                     /pseudo
     CDS             50..142
                     /locus_tag="ACHJ1G_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            155..244
                     /locus_tag="ACHJ1G_005719"
     CDS             155..244
                     /locus_tag="ACHJ1G_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767319.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            264..>998
                     /gene="nleL"
                     /locus_tag="ACHJ1G_005720"
     CDS             264..>998
                     /gene="nleL"
                     /locus_tag="ACHJ1G_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4767320.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLEN"
BASE COUNT          296 a          168 c          227 g          307 t
ORIGIN      
        1 atcctgcgtg ctgacgggac gtgggaaaat attggaggga tgaagtaacc cgacagcttc
       61 acaaaaccgg agtccggctc cggtttttgt tgtcatgtcc ggggaatatt tgttagataa
      121 aaaagaggag ataattcaat agggagttaa attaatgccg ataaatctga catcttattt
      181 gtgggtacag ggacagaaag ttgtcccggc agttgtgttt tctaatttta ctttagtgga
      241 ctaagtaaaa aggagtgaga taaatgctgc ccactacaaa tatctctgta aattctggag
      301 taatatcttt tgaaagtcct gtagattcac catctaacga ggatgttgaa gttgccctcg
      361 aaaagtggtg cgctgaggga gaatttagcg aaaatcgtca tgaggttgca tcaaaaatac
      421 ttgatgttat aagtactaat ggagagactt tatcaatcag tgagccaata acaacattac
      481 cagacttgct tccaggttct ctgaaagaac tggttttgaa tggatgtaca gagcttaaat
      541 caataaactg cttacccccc aacttatctt cattaagtat ggttggatgc tcatcattag
      601 aggttataaa ttgcagcata cctgaaaatg tcattaattt atctttatgc cattgtagtt
      661 ctttgaaaca tatagaaggt tcctttcctg aggcactcag aaattccgta tatttaaatg
      721 gctgtaattc attaaatgaa tcgcaatgtc aattccttgc atatgatgtc agtcaaggcc
      781 gtgcctgcct gagcaaagct gagcttactg ctgacttaat ttggttgtcg gctaaccgaa
      841 cgggtgaaga gtctgctgaa gaattgaatt actctggatg tgacttgtca ggtctaagtc
      901 ttgtagggct gaatttatca tcagtaaatt tttctggagc agtgcttgat gatacagatc
      961 tcaggatgag tgatttgtct caggctgtat tggaaaac
//
LOCUS       DAZFDP010000479          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFDP010000479 DAZFDP010000000
VERSION     DAZFDP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..732
                     /locus_tag="ACHJ1G_005721"
     CDS             <1..732
                     /locus_tag="ACHJ1G_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO4767321.1"
                     /translation="RFNIWCQGATPWMGNGAWAECAGTFTEEDLHGQECYAGLDLSST
                     SDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRAVYRQWAKAGWIRTTPGDCID
                     YDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAGFEVEPFPQTYLRFSPAAKSF
                     EVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNKKKSPNKIDPSVAALMAFGTF
                     QAEHEDFAFDISDSHRQKLEEFSGV"
     gene            746..>996
                     /locus_tag="ACHJ1G_005722"
     CDS             746..>996
                     /locus_tag="ACHJ1G_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767322.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITY"
BASE COUNT          252 a          234 c          294 g          216 t
ORIGIN      
        1 cgatttaata tctggtgcca gggggctacc ccgtggatgg gtaacggcgc atgggctgag
       61 tgtgccggaa cgttcacgga ggaagattta cacgggcagg agtgttacgc ggggctggac
      121 ttatcatcaa ccagcgatat ttccagcgtg tgctatgcct ttccggtcgg taaaaatatc
      181 atgctggttt cccgtcatta tctgccggag ttccagctac agaaccccgc caataaaaac
      241 cgcgctgtct atcgccagtg ggctaaagcg ggctggatac gcacaacgcc aggtgactgc
      301 attgattacg accggatcag agatgacatc atgcaggatg cggagaaatt taatatcagg
      361 ctggtgggct ttgatacgtg gaacgccacg catctcagga cgcaattaca gggggctggt
      421 tttgaggtgg agccgttccc gcaaacctac ctcagattca gtccggcggc gaaatcgttc
      481 gaagtttttg ttaaccgcag ggtgattgtg catcgtggcg atccggtgtt gtcctggtcg
      541 atgagtaacg tcgtgatgca gagtgacgcg aacgccaata tcaagccgaa caagaaaaaa
      601 tcaccgaaca agatagaccc gagcgtagcg gcgctgatgg cgtttggtac attccaggca
      661 gagcatgagg attttgcttt tgatataagc gacagccacc gccagaaact ggaagaattt
      721 agcggggtgt aatgaggtca gcagcatgac agaagccgaa atactgcgat taatccgccg
      781 cgtctctgga atcagccagc aggctgacga acagaccacg cagccggaca gcgtgacagc
      841 cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt gatggtatcc agcttaatga
      901 tgcggatatg cgcgacatac ggatccgcgt tcttgaaatg ctggcctaca atcgccgcgt
      961 tgaactgtat cgggagaagg agaaaatcac gtacca
//
LOCUS       DAZFDP010000480          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFDP010000480 DAZFDP010000000
VERSION     DAZFDP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..848)
                     /locus_tag="ACHJ1G_005723"
                     /pseudo
     CDS             complement(1..848)
                     /locus_tag="ACHJ1G_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            825..995
                     /locus_tag="ACHJ1G_005724"
                     /pseudo
     CDS             825..995
                     /locus_tag="ACHJ1G_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          238 a          291 c          259 g          207 t
ORIGIN      
        1 ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg agaggtcagc
       61 acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtacgtggg tggcggcgct
      121 catgtcaaac tcaccctgca tccagcagac ggccagcaga acgtttttgg gatttttctg
      181 caatgccgct tttgtgcggg aaatcagatc ctgatataac ggcttgccca ccccccagcg
      241 tgccgaatcc tgactggccc ccgtggactc gctgaatgtc ccctccgcgc cctgggtaaa
      301 tgccgaacca ccacgacagc atggtaccag caggatcccc gcgttattcg ggatatacgg
      361 gagcagtttt ttggcaatat gtaaaccctg gccgacacag ccgtactgcc ctttgctcag
      421 gtcagccctc ggatgattca gcgtactcat atcctgcaca tcatgcagac agtggtcagc
      481 cggaataata tcgttatatc tgcaggcagc cccgcccggc gtcactgtac tgcggcgcgc
      541 cagctgttta atgcgcggat ccggagcatc gtatgaatcc ggcagcggaa gcccttcacc
      601 gtaagccctt caccgtaagc catggcattg gattgcccgg ccagtacgat gacgtagtac
      661 caatccggct cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc
      721 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      781 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta
      841 atcgacatcg aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa
      901 atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc
      961 cagaactcac aaggaaaatg ataaaaggaa taaca
//
LOCUS       DAZFDP010000481          988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFDP010000481 DAZFDP010000000
VERSION     DAZFDP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..>988
                     /locus_tag="ACHJ1G_005725"
     CDS             125..>988
                     /locus_tag="ACHJ1G_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4767323.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLDEELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDW"
BASE COUNT          296 a          240 c          243 g          209 t
ORIGIN      
        1 acctgaataa cgaaataccg gctggataac ccgccagcca tattaacgcc ataccaacgg
       61 attaaaaatg ccagcaatgg cagggattcg ttcaccctga aatctgtaat gaggttaaaa
      121 caaaatgagt aaagtcttta tttgcgccgc cattccggac gaacaggcaa taaaggaaga
      181 aggtgccgtc gctgtagcca ctgccattga agccggtgat gaacgtcgcg cccgcgcaaa
      241 atttcactgg caattcctgg aacattatcc ggctgctcag gactgcgctt ataaatttct
      301 tgtctgcgag gataaacccg gtataccccg ccctgccctc gattcctggg atgctgaata
      361 tatgcaggaa aaccgctggg atgaggaatc cgcttccttt attccggtcg aaccagaatc
      421 cgatccgatg aacgtcaatt ttgacaagct gtcccttgaa gtacagaacg cggtcctggt
      481 taagttcggt acatgtgaaa acatcaccgt tgatatggcg attgacgcgc aggaattact
      541 gcaggaagac gttgctacct ttgacgggca tatcgttgaa gcactgatga aaacgcctga
      601 aattaacgct atgtatccgg aacgcaaact gttcgctatc ggatgggtta aacacaaatg
      661 taatccgggt gccaaatggc ccgaaattca ggctgaatta cgtaactgga aaaaacggca
      721 ggacgcagag cgcaaagaga ctggaaaata cacgtctgtt gttgatctcg ccagcgccag
      781 agtcaatcaa cagaacactg aaaactcagc aggaaaaatc aacccagtca ctgccgccat
      841 ttgtcgcgaa tacaagcaga catggaaaac gctggatgaa gaactggcct acgctctctg
      901 gcctggtgat gtggatgccg gaaacattga cggcagcatc catcgctggg caaaaaatga
      961 agttatcgac aacgaccgcg aagactgg
//
LOCUS       DAZFDP010000482          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFDP010000482 DAZFDP010000000
VERSION     DAZFDP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..>983)
                     /locus_tag="ACHJ1G_005726"
     CDS             complement(91..>983)
                     /locus_tag="ACHJ1G_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4767324.1"
                     /translation="VLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQP
                     GQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLT
                     TNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNF
                     TWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATT
                     DSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQT
                     GNKVSAGTVQSVIGVTFIYE"
BASE COUNT          268 a          228 c          209 g          278 t
ORIGIN      
        1 ttcttacagg tgtaggctaa tagccttcac aaaataatta tccgcgcaat tttcgtgaat
       61 ggatcgcata gactaaggtc aaaatcacaa ttattcatag ataaaagtga caccaatgac
      121 tgactggacg gttccggctg aaaccttgtt tcctgtttgt ccataggttg cgcttaaacc
      181 cagaggtact ttgcttttat ttactgtccc cagggagaca ttttctcccg tagccagtat
      241 ttttccatta cgcattagag tgacacccac tcccgatgct tttgttgcat caggtgctgt
      301 gttagcaaac acttgtcggc tactgtcagt ggtggcaccc gaaagataaa atgagagctt
      361 ttgttcgctt gagcaatata cgccaagggg tatttctgcg cttccaggaa agtccgggag
      421 atccacggtg acattacgac tatcaacggt gcagccgcct gttggcataa caacattgtt
      481 atttgaaata atattccacg taaaattacg cggatttccg cttcctaatg ttgcaatttt
      541 atacatatgt atacgggcaa tgacttcccc cgcttttatg acaactcctc cggccgcccc
      601 aactggggta atatagagtt ttaacggtaa tggcatcggt gtcttatcac caatatccaa
      661 tacgttggta tttgtcgtca acgggaaggg gtaggtcaca ttattccagt acagcgatcc
      721 tttatatgac tgtagggagc ctgcgaaagc tgagccttgt accaggttta tatgatcagt
      781 gtcgtaccag ccgccgtaat cattccagca acttatatgc tgagacaagt ctacaaccag
      841 attctggccc ggctgtatta cagggtcgag attaacataa acagaggttg tacctgctcc
      901 aatacttgac ccgccatcga cattacaact gaaggcaaaa actttgcctg ccattaatag
      961 aagataaata ccgaacagga cct
//
LOCUS       DAZFDP010000483          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFDP010000483 DAZFDP010000000
VERSION     DAZFDP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..947
                     /locus_tag="ACHJ1G_005727"
                     /pseudo
     CDS             1..947
                     /locus_tag="ACHJ1G_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          284 a          225 c          252 g          208 t
ORIGIN      
        1 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aacagcgtat
       61 tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa caatttccca
      121 ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac tgggtacagc
      181 atgaaccctc agacagaaaa caaagtgatg agcggctaaa actggagatt aaggtggcac
      241 atatccgcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg gcagagaatg
      301 gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg ctacgctgta
      361 agcagaaacg caagttcaga gcgactacga actcgaacca caatctgcca gttgcgccaa
      421 atctgctgaa ccagacgttc gctcctacag caccaaatca ggtctgggtg gcggacctga
      481 cgtatgttgc cacacaggag ggatggttgt acctcgctgg catcaaagat gtttatacgt
      541 gcgaaattgt cggctacgcc atgggagagc gcatgacaaa agagctgaca ggtaaagctc
      601 tgtttatggc gctcaggagc cagcgcccac ctgccgggct aatccaccac tctgatcgag
      661 gttcacagta ctgcgcatac gattaccggg tcatacagga gcagtttggt ctgaaaacat
      721 caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc tggggaacgc
      781 tgaaaaatga gagcctgagc cactatcgtt ttaataaccg ggatgaagcc atctcagtaa
      841 tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata
      901 tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa aagaacaaat
      961 ggtagtgtc
//
LOCUS       DAZFDP010000484          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFDP010000484 DAZFDP010000000
VERSION     DAZFDP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..404)
                     /locus_tag="ACHJ1G_005728"
     CDS             complement(<1..404)
                     /locus_tag="ACHJ1G_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO4767325.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLS"
     gene            585..737
                     /locus_tag="ACHJ1G_005729"
                     /pseudo
     CDS             585..737
                     /locus_tag="ACHJ1G_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(762..>968)
                     /locus_tag="ACHJ1G_005730"
     CDS             complement(762..>968)
                     /locus_tag="ACHJ1G_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767326.1"
                     /translation="AKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLN
                     VAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          307 a          170 c          184 g          307 t
ORIGIN      
        1 aaagataaat ttaattgttg gctatgaaaa ttgctgccat ttccatatct aaaatgttca
       61 atcagatctt cgataagtgt tttatattgt ccaaaaaagg aaagcatttt tgctagttct
      121 ttcatttctg caaataaaat atcagtgcat tcacgttgtg atatttttgt tccggaactt
      181 gaggcactaa aagcaacatt aatagaacct gggccaggga aatggtattt aataagtctg
      241 taaggatcaa cttttgcaga gatatcgctt aggccgagtg agagaaagtc ttgttctacc
      301 atatcaccat attgcatatc atcagcagaa aaatcatcga ataaactaat agtttcgaaa
      361 agtgtacagg gaatattaat tggcggaagt aatatgttat tcatttgcat ttgccttggt
      421 tatatttaag ctcaatatca attcgagaac aaatgctagg gtaaaaaaat atttaaaaaa
      481 gaagtaaact tcacattttt tcattacatc tctgaagaga acctcgttaa tgattggtct
      541 tgctcattgt taataacaaa atgttgaatt taccgattag gaaacttttt cctacgcgga
      601 ttgcctccag ctatggcatg aactttgagt ttatgccaga actgaagtgg ggcttcggct
      661 accctggcgc gattatcttt atgatcctcg cgggcctgac accgtatctg tactttaagc
      721 gtaagaactg gctgtaacta tacgatgtcg ctgtgagtaa gttagtgtgc tgacagcgac
      781 attttagccg agggaatcgc agataacata attgttgtga ataatatcca tgccacgttg
      841 agttggacgc ttaatgactc acatctcacc ccttcaattg ctgcagagat aatagaaaaa
      901 agtgtcaagc ccaatgaaat caatacaaag tagattgcct tacatccagt atctcttatt
      961 ctttttgc
//
LOCUS       DAZFDP010000485          965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFDP010000485 DAZFDP010000000
VERSION     DAZFDP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>965
                     /locus_tag="ACHJ1G_005731"
     CDS             <1..>965
                     /locus_tag="ACHJ1G_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4767327.1"
                     /translation="IPNNAGILLAPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGK
                     PLYQDLIARTKAALQKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAMLKQFRADLT
                     VFNAQCHGGSAVNVPWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGN
                     GVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAIL
                     NAAGRTSAFISGKAPEIKPSPGGDTPSGPSADTSVRTISLLPTAGEAAAQGWTIKDGG
                     IQLSGGVFKIAKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGA"
BASE COUNT          226 a          256 c          287 g          196 t
ORIGIN      
        1 atcccgaata acgcggggat cctgctggca ccatgctgtc gtggtggttc ggcattcacc
       61 cagggcgcgg aggggacatt cagtgcggac acgggggcca gccaggattc ggcacgctgg
      121 ggtgtgggta aaccgttata tcaggacctg attgcgcgca ctaaagctgc attacagaag
      181 aacccgaaaa atgtgttgct ggcggtgtgc tggatgcagg gagagtttga catgagcgcc
      241 gccacctacg cacagcaacc tgcgctgttt acagccatgc tgaagcagtt tcgtgctgac
      301 ctcactgtgt ttaacgcgca gtgtcatggt ggcagtgctg taaatgtgcc gtggatttgt
      361 ggtgacacga cgtattactg gaaaaataca tacgctaccc agtacgacac cgtgtacggc
      421 gggtataaaa acagggagag tgagggcgtt tattttgtgc ccttcatgac agacggtaac
      481 ggcgtcaata ccgccactaa cgcaccggca gaagatccgg atattccggc atcaggatat
      541 tacggtgcgg catcgagaac gaatggaaac caggtatcat caaaccgccc gacacatttc
      601 agttcatggg cgcgcaggag cattattccg gatcgtatgg caaccgctat tctgaacgca
      661 gccgggcgca cctcagcttt catcagtggt aaggcaccgg aaatcaaacc ctcgcccggc
      721 ggcgacacgc catcgggtcc gtctgcagat acgtccgttc gcacaatctc cctgctgccg
      781 acagccggag aggctgctgc gcagggctgg accattaagg acggcggaat tcagttgtcg
      841 ggtggtgtat ttaagatcgc caagcagagc aataaaacct ggtccctgac gcatccggtg
      901 gatgacgcaa ttaccctgct gacacagggc ggcagactga cctgtaagtt ccgcctgtca
      961 ggcgc
//
LOCUS       DAZFDP010000486          964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFDP010000486 DAZFDP010000000
VERSION     DAZFDP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..291)
                     /locus_tag="ACHJ1G_005732"
     CDS             complement(<1..291)
                     /locus_tag="ACHJ1G_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO4767328.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQM"
     gene            complement(430..663)
                     /locus_tag="ACHJ1G_005733"
     CDS             complement(430..663)
                     /locus_tag="ACHJ1G_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767329.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            777..>964
                     /locus_tag="ACHJ1G_005734"
     CDS             777..>964
                     /locus_tag="ACHJ1G_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4767330.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAA"
BASE COUNT          271 a          210 c          206 g          277 t
ORIGIN      
        1 catctggatt tgttcagaac gctcggtctt gcacaccggg cgttttttat tggtgagtcc
       61 atcaagcgca tacttaaaag ctctgctaat cggactgatg tctgatgcca ttccgaaagc
      121 acacaagacc gaagcaataa atctccagtc cgttctgctt atcttcgatt catgacagcc
      181 aatcatcttt gccagaccgc gctgggtaag cgttgacagg ttgataagta aatctgtttc
      241 tgcgcgatca acatcacgct gtgatagttt gctgtaactt gtttgttcca tttcttaaga
      301 tttccaatag tgaatagcta gttgaaaggt atgcgtggaa acgcatatgg ccttagttgg
      361 tcagatatct tggaactcgc ttttcagcga cgtaggacga atgtccgttg ttacaaagag
      421 cggggttact tatgctgctg atgctctacg cgatacgaac accagatttt cctttttcac
      481 aggtttataa cccgtgaaat tacgagtggc ttcttcgatt gcattcgctt tatctgggga
      541 agctcttcga aacccatata caatctgatc aagatagcca actgaagtat tcgctaatgc
      601 ggcgagtcgc ttccattcct cactagaagc ctcttttcgc cagcgtagta gttcattact
      661 cattagtgcc tccgtttatc acacagagcg actttaccat tttgataaat aaaacgcaat
      721 gaagatttat caaaatgcat atttatccat ttgctaaata gagggagaat cgtgggatgg
      781 aaaacaaaga tattcgcaaa tcgaatctgg cgtttttgct agatgagcat aaaaaaatcg
      841 cgggtaacac taatgcaagc tttgccgata agcttggagt tagcccttct caactcacgc
      901 aagtctccgg tgaaaaaagc actcgaaaca taggggataa actagcaaga aaattcgaag
      961 ccgc
//
LOCUS       DAZFDP010000487          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFDP010000487 DAZFDP010000000
VERSION     DAZFDP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..377
                     /gene="dicB"
                     /locus_tag="ACHJ1G_005735"
     CDS             189..377
                     /gene="dicB"
                     /locus_tag="ACHJ1G_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4767331.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            374..562
                     /locus_tag="ACHJ1G_005736"
     CDS             374..562
                     /locus_tag="ACHJ1G_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4767332.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            658..>953
                     /locus_tag="ACHJ1G_005737"
     CDS             658..>953
                     /locus_tag="ACHJ1G_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4767333.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
BASE COUNT          265 a          203 c          221 g          264 t
ORIGIN      
        1 ctgctctgcc gcccttttta aagtgaattt tgtgatgtgg tgaatgcggc tgagcgcacg
       61 cggaacagtt aaaaccaaaa acagtgttat gggtggattc tctttatccg gcgttaattg
      121 ttaactggtt aacgtcacct ggaggcacca ggcactgcat cacaaaattc attgttgagg
      181 acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc tgaaggttgt tttgatattg
      241 gtgttctgct cagtaaccgg gagtttactg aagatgccat taatatgagg aaatatgagc
      301 cttatctgct caatgataat tccatacttt cccgaattgc tcttcttgaa cttggtattt
      361 tcggagaacg tcaatgactt cagcatttgc actgatgatg acggtttttc ttataacggg
      421 tgaatcacag aatgtgatta ccggaattta tgcaagtaaa gaatcctgcc tccaggcaag
      481 agacgagcaa aaaatttctg gtgaatgcct cccgctaaaa aaagtatcgc tgtacctgaa
      541 taacgaaaca ccggctggat aatcctccag ccatattaac accataccaa cggattaaaa
      601 atgccagcaa tggcagggat tcgttcaccc tgaaatctgt aatgaggtta aaacaaaatg
      661 agtaaggtct ttatttgcgc tgctattcct gatgaacagg ccataaaaga agatagcgct
      721 gttgcggtgg ccactgccat tgaagctggt gatgagcgtc gcgcacgcgc aaaatttcat
      781 tggcaatttc tggagcaatt ccctgcagct caggactgcg cttataaatt tattgtctgt
      841 gaggataaac ccggcatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      901 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg ttgagactga atc
//
LOCUS       DAZFDP010000488          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFDP010000488 DAZFDP010000000
VERSION     DAZFDP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..432
                     /locus_tag="ACHJ1G_005738"
     CDS             76..432
                     /locus_tag="ACHJ1G_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4767334.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(483..>935)
                     /gene="yhaJ"
                     /locus_tag="ACHJ1G_005739"
     CDS             complement(483..>935)
                     /gene="yhaJ"
                     /locus_tag="ACHJ1G_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4767335.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT          224 a          230 c          206 g          275 t
ORIGIN      
        1 aaaagaaaga tacgaatagc atttacacaa tacccggaat agactttcgt cacttttaat
       61 taaagggatg tttttatgca gtggtattta gccgtattaa aaaattatgt tggtttctct
      121 ggtcgcgcac gtcgtaaaga gtactggatg tttactctga ttaacgccat cgtcggcgct
      181 attatcaatg tcattcaatt gattttaggt ttagagtttc cattcctttc tctcatttac
      241 ctggcagcaa caatcattcc ggtcattgct ctgtgtgttc gtcgtctgca cgatactgac
      301 cgttcaggtg cgtgggcgct tctctatctg gtaccgatca ttggctggtt agttctgttt
      361 gtatttgcct gtttagaagg caattctggc agcaatcgtt acggaaacga tcctaagttt
      421 ggttcaaatt aatcttagaa ttggggcgac atttcgcccc tttttattaa taataacaaa
      481 tattattttc cgctaaaaag tttgggaatt tcccgcagac accaggattt cgcttccccc
      541 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
      601 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
      661 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
      721 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
      781 attccacgat atttcacgcg cgtcacttca gataacggtt ccggctcctg atgaatcggg
      841 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
      901 gacgaggaac gaaaatgcat atccggcgcg ataac
//
LOCUS       DAZFDP010000489          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFDP010000489 DAZFDP010000000
VERSION     DAZFDP010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /locus_tag="ACHJ1G_005740"
     CDS             <1..117
                     /locus_tag="ACHJ1G_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HFO4767336.1"
                     /translation="RRIEYTAASLDDLNRAINDAESVLGTTSRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHJ1G_005741"
     CDS             114..>931
                     /locus_tag="ACHJ1G_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767337.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          191 a          216 c          322 g          202 t
ORIGIN      
        1 cgaagaattg aatatacggc ggcctctctg gatgatctta accgggcgat caatgatgcg
       61 gagtcagtgc tggggacaac aagccgccgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggtg ttccgcttcg ggagagcctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gttgcccgcg ttgcgtctgg gcaatgcccg tgcagatgat ctggtgcgca
      301 ataacgggat agcggccaat gcggtggcac tgcataagga tcatattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagact gcagcaaaaa
      421 gttttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga agggaaacgc acgtttacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggagagcacg caactgtttc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctctggta gtggacgatg ggaacggatc ccgcgtgaac
      781 tacccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggctaa tcagttttac agcgtcatgg aacggctgaa gatgctggat tccctgcagg
      901 caacacagct tcagtcggcc attgtgaaag c
//
LOCUS       DAZFDP010000490          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFDP010000490 DAZFDP010000000
VERSION     DAZFDP010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>928
                     /locus_tag="ACHJ1G_005742"
     CDS             <1..>928
                     /locus_tag="ACHJ1G_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4767338.1"
                     /translation="RDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHM
                     MVFAHPEEIDTIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGK
                     NLDSHAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWR
                     ELRRLASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTA
                     YDLADELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSAHENGFDL
                     QGGPAAPWTRGNNCPRVQETDNNGTEQPEERPA"
BASE COUNT          222 a          280 c          263 g          163 t
ORIGIN      
        1 tgcgcgactc cagcgattac ctggttaaca cgttttttgc ggctgtccgc aaaaaactga
       61 accgcgacgg tctgcgctgg tatggcatcc gcacggtgga gcctcaccat gacggcaccg
      121 tgcactggca tatgatggtc tttgctcatc cggaagaaat cgacaccatt gtgtcccaca
      181 cccgcgatat tgccattcag gaagaccgcc acgagctggg cgatgacata actccgcgct
      241 ttaaggcgga gtacgtcgac ggctcaaaag gcacaccaac cagctacatc gccacctaca
      301 tcggaaagaa cctggacagc cacgccgtgg atggcatcga cccgaaaacg ggcaagccac
      361 gcgttgacca cgaaaccgga aaatcaatgg ccgagagcgt ggaacgcgcc atcggctggg
      421 cgcgccttca ccgggtccgc cagttccagt tctttggtat cccctcccgt caggtgtggc
      481 gtgaactgcg ccgccttgcc agccagatgg cacgcaaccc ggaaggcccg caacggctga
      541 aggatgacgc aatggacgcg gtactcgctg ccgctgatgc cggatgtttt gccacctaca
      601 ttgagaaaca gggtggcgta cttgttccac gcaaagacta cctgattcgc accgcctacg
      661 acctcgcaga tgagctgaac gattacggtg aacagagtgt acagatttac gggatctggt
      721 cgccgctcat tggggagtct tcccgcgtat gcacgcatcc ggataactgg aagctggtaa
      781 gacgcaaacc ggaagcggaa gacagcgccc acgaaaatgg ttttgacctt cagggcggcc
      841 ctgccgcccc ttggactcgt ggcaataact gtccccgtgt acaggaaacg gacaacaacg
      901 ggacagaaca gccggaagaa cggccagc
//
LOCUS       DAZFDP010000491          917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFDP010000491 DAZFDP010000000
VERSION     DAZFDP010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..>917
                     /gene="cif"
                     /locus_tag="ACHJ1G_005743"
     CDS             151..>917
                     /gene="cif"
                     /locus_tag="ACHJ1G_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO4767339.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKH"
BASE COUNT          281 a          183 c          203 g          250 t
ORIGIN      
        1 cgcgtcacag aaatggacaa tgccgctgag agactggcga atggcataca ggcaccagaa
       61 aaatttcaga catgacataa tgcccatgcc agaagttctt tttgcacctc gcctgtattt
      121 atagataaat atagagtgaa tgaatgagat atgaaagaca ttacccttcc ccccccgacg
      181 tccgcgtcct gtctgacagg ggccatatct gtaaatactg aagctgtatt atctcccatg
      241 caacacactt cagccttaca tgtaagagat tttgcttccc tgtgctcaca gaacctcaaa
      301 gctaatgtat tgctaaatag tgatgaccac gaagtaccta tacatcagaa aaatcctgct
      361 gcaataatgc aaaatatcga ctctaacatc aaacagatgg caacagactg ggggatgtcg
      421 attgaggagg ttgaggttat tatagggcga gagaaaggca ttgtggaacc ctcctgcgga
      481 gttaccgcta atgctattat gaaactattt ctggacaagg atggcttcag ttactgcttt
      541 gaaaatgaac agacactatc gctcgagcag cttcaggagc gcctgtcctg tatgcctgaa
      601 tgtaagagct ttgtattacg tgttaatgat ggtgcgcttg gtcatgctta cattgtcgat
      661 attcccaaag gagaaaactc ttgtcgtcct gcattcttgt atcagtcaga tttaggagag
      721 ggcgtcacca gaaagttaag atttgaggac tggatgacgc ataaagcatt gactccgatt
      781 ttgctggatg atatttgtaa ttacttctcc tgcatgtctc aaaataagac agatttggag
      841 cagattgcaa cgttatttga tattgatgga aatgttaaaa tgttacgaaa ggaaaatatt
      901 caatatcaaa agcatga
//
LOCUS       DAZFDP010000492          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFDP010000492 DAZFDP010000000
VERSION     DAZFDP010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>916
                     /locus_tag="ACHJ1G_005744"
     CDS             <1..>916
                     /locus_tag="ACHJ1G_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4767340.1"
                     /translation="TVNLIKSARVGYTKMLLGVVGYFIEHKSRNSLLFQPTDSAAEDF
                     MKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSV
                     DVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANES
                     AHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQ
                     SNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDA
                     LKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          210 a          230 c          260 g          216 t
ORIGIN      
        1 gcactgttaa tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggggtgg
       61 tcgggtattt tattgagcat aaatcccgaa acagtctgct ttttcagccc acggattctg
      121 ccgctgaaga ttttatgaag tctcacgtgg aggcgacgat tcggaacgtg ccatgcctga
      181 aagacctttc cccatggctg ggtcgtaaac atcgtgacaa tactctcacg ctgaaacgct
      241 tttcatcggg cgtcggtttc tggtgcctgg gcggcgctgc cgccaaaaac taccgtgaaa
      301 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgagccggat gtcgaaaaag
      361 agggctcgcc aaccctgctg ggggataagc gtattgaggg gtcggtgtgg ccaaaatcca
      421 ttcgcggctc gacgcctaaa atcaaaggca cctgccagat cgaaaaagcc gctaacgagt
      481 cggcgcattt tatgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
      541 aatttggcga tgagtccacg ccttttgggc ttaaatggga gaaggacagc cctgaaagtg
      601 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgaactg gaccagagca
      661 acgggcggtg gatctgtgaa aacacgggca tgtggacccg tgacggtctg acgtttttca
      721 gcgcccgggg tgatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
      781 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
      841 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
      901 gcgaaaaact cgatca
//
LOCUS       DAZFDP010000493          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFDP010000493 DAZFDP010000000
VERSION     DAZFDP010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..490
                     /locus_tag="ACHJ1G_005745"
     CDS             <1..490
                     /locus_tag="ACHJ1G_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767341.1"
                     /translation="GEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVT
                     AKEVITRTVKVTNVGKPSVAEERSEITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESA
                     TDKTFRAVSADPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATVAEVTVTEAGA
                     AG"
     gene            504..>915
                     /locus_tag="ACHJ1G_005746"
     CDS             504..>915
                     /locus_tag="ACHJ1G_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4767342.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNALTDDDFSAG"
BASE COUNT          229 a          204 c          289 g          193 t
ORIGIN      
        1 gggagaagaa ggtcagaaag ggcttatagg ctggtttgaa agcggggatg tgcgggccta
       61 taaaatccgt ttcccgaacg gcacggtgga tgtgttccgt ggctgggtca gcagtatcgg
      121 taaggccgta acggcgaagg aagtgatcac ccgtacggtg aaagtgacca acgtgggtaa
      181 accttctgtg gcggaagaac gcagcgaaat tacgccggtc actgcgatta aggtgacgcc
      241 gacatctggt acggtggcaa aagggaaaac aacaaccctg acggtttctt ttgagccgga
      301 aagtgcaacc gacaaaacgt tcagagcggt ttccgccgat ccgtcaacgg gaaccattgc
      361 tgtgaaagat atggcgatca ctgtgacggg ggttaaggct ggaaaagtga gtatccccgt
      421 gatttccggt aatggtcagt ttgccacggt agctgaagtc accgttactg aagcgggcgc
      481 tgcagggtaa acggaggtaa tacatgtttc tgaaaaccga acaatttgaa tataacggtg
      541 tgtccgtcac gctttccgag ctgtctgcgc tgcagcgtat cgagcatctt gccctgctga
      601 aacgacgggc agaacaggct gaagccagcg gtaacctgca ggtgagcgtg gaagaccttg
      661 tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca taaccatcca cagaaaacgg
      721 agtcaccatc aatgaatgag gctgtgatgc agatcgaaca ggaggtgctc accacctggc
      781 ctgctgatgc cattgcccgg gcggaagacg tggtgttgcg tctgtccggg atgagcgggg
      841 ctgttcatgt ggatacggat atcaccgaag tggcgaaaaa taacgcgctt actgatgatg
      901 atttttctgc gggaa
//
LOCUS       DAZFDP010000494          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFDP010000494 DAZFDP010000000
VERSION     DAZFDP010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>907)
                     /locus_tag="ACHJ1G_005747"
     CDS             complement(<1..>907)
                     /locus_tag="ACHJ1G_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4767343.1"
                     /translation="KPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIG
                     KTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQV
                     DTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMVNPANGN
                     TKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNAN
                     AGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDD
                     QPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          196 a          260 c          212 g          239 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggctt
//
LOCUS       DAZFDP010000495          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFDP010000495 DAZFDP010000000
VERSION     DAZFDP010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..903
                     /locus_tag="ACHJ1G_005748"
     CDS             <1..903
                     /locus_tag="ACHJ1G_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO4767344.1"
                     /translation="PWVNALPPMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLNRSA
                     EYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYENDEL
                     TYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVHAAS
                     LERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVK
                     ATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDT
                     ADRLNYQKMAQVVDGVINLLYNSK"
BASE COUNT          243 a          212 c          213 g          239 t
ORIGIN      
        1 ccctgggtca atgcattacc gccaatgccc cgacacgcaa atcctgagca attagaaaaa
       61 acagtgcgtt atcttacaca aactgtgcat ccacgtagtg ccgacaatat tgataatctg
      121 aataggtccg cagagtatat aaaagaggtc ttcgtcagta gcggtgccag aattacctcg
      181 caggacgtcc ccattacggg tggcccctac aaaaacattg ttgctgatta tggtcctgcc
      241 gatggaccgc tgattattat tggtgcgcac tatgactctg ccagcagtta tgaaaacgat
      301 gaattgacct atacacctgg cgcggatgat aacgccagcg gcgtggcagg attactcgaa
      361 ctggcacgtt tgttacatca gcaagtaccg aaaacaggcg tgcagctggt cgcctatgcg
      421 tcggaagaac cgcccttctt tcgtagcgat gaaatgggga gcgcggtaca tgcagcttcg
      481 cttgagcgtc cagtaaaatt aatgatagca ctggagatga ttggctatta cgactctgcg
      541 cctggtagcc aggattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      601 tttattgccg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcgaca
      661 ttgttatcat cccggtattt atctgtttat tctatgaatg ccccagggtt tattcccggt
      721 attgatttct ctgatcacct taattattgg caacacgata ttcccgccgt aatgattact
      781 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
      841 aattatcaga aaatggctca ggtggtggat ggtgttataa atttgttata caacagtaaa
      901 taataaa
//
LOCUS       DAZFDP010000496          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFDP010000496 DAZFDP010000000
VERSION     DAZFDP010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..717
                     /locus_tag="ACHJ1G_005749"
     CDS             106..717
                     /locus_tag="ACHJ1G_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO4767345.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          301 a          149 c          192 g          261 t
ORIGIN      
        1 cttgtagata gtgccggagg aaaatttaaa tataaaacaa ttattgtttt taatgaaaat
       61 aaaaaacatt ttgttatcgt tatataatat ataaaggttt attgtatgtt tagtccaatg
      121 acaatggtag gtagatcgtt agctcaggcg gctacacaaa ctcttagacc tgcggtcacc
      181 agagctgcga cacaagcggg ggtgggagcc agtggcatga agttcgtgcc catgcagtcg
      241 aactttatga ttaatcatgg caaactgact aaccagctac ttcaggccgt ggccaaacaa
      301 acccgtagcg gtgatactca gcaatggttc aaacaggagc aaattactta tatatccaga
      361 acagtaaaca gagctattga tgactattgc atgagtaata attcggtaat tggtaaggaa
      421 accaaaagcc acattttcaa ggctgttgaa agcgcgataa agcagcctct cgatatgaat
      481 tgtgcacaaa cgtcgattgg ccatttctta cagtccaaca aatactttaa taaaaaagtt
      541 gatgagcagt gtggcaaagg ggtggatcca attacacgct ttaacaccca gaccaggatg
      601 atagaacaag tctcacaaga aatctttgaa cagaaatttg gtggtttgaa agttagtgat
      661 atcaaagctc tgactcagag cacgattgca cagcatgtac aggatactcg gttgtaggaa
      721 taacctgtta tatatgcgtg ttgggcataa gttaagcttc ctggtgtata gcgtagtctt
      781 tgctgatgta agaaaaggct atatacggaa acttgatata ctataaactt cattatttat
      841 tatattgaaa aagttattta attgatttgt tttgtaattt taatgcttca attggataag
      901 cat
//
LOCUS       DAZFDP010000497          901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFDP010000497 DAZFDP010000000
VERSION     DAZFDP010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>901
                     /locus_tag="ACHJ1G_005750"
     CDS             <1..>901
                     /locus_tag="ACHJ1G_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767346.1"
                     /translation="TKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHV
                     PGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVS
                     VEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQ
                     HVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVK
                     GVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDAAS
                     VSFRIAAPAAPSRIELTPGYFQIT"
BASE COUNT          188 a          243 c          314 g          156 t
ORIGIN      
        1 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
       61 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtcgg cgcagaaggg
      121 cttcgccatg taccgggcga tgttattgaa atctgcgatg atgactatgc cggtatcagc
      181 accggtggtc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
      241 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
      301 gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
      361 gagggtgttg ctgaatacag cgtatggggg ctgaagctgc cgacgctgcg ccagcgcctg
      421 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
      481 catgtgccgg aaaaagaggc catcgtggat aacggggcgc actttgacgg cgaacagagt
      541 ggcacggtga atggtgtcac gccgccagcg gtgcagcacc tgaccgcaga agtcactgca
      601 gacagcgggg aatatcaggt gctggcgcga tgggacacac cgaaggtggt gaagggcgtg
      661 agcttcctgc tccgtctgac cgtaacagcg gacgacggca gtgagcggct ggtcagcacg
      721 gcccggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
      781 acagtccggg cggtaaatgc gtgggggcag cagggcgatg cggcgtcggt atcgttccgg
      841 attgccgcac cggcagcacc gtcgaggatt gagctgacgc cgggctattt tcagataacc
      901 g
//
LOCUS       DAZFDP010000498          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFDP010000498 DAZFDP010000000
VERSION     DAZFDP010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..>895)
                     /locus_tag="ACHJ1G_005751"
     CDS             complement(33..>895)
                     /locus_tag="ACHJ1G_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO4767347.1"
                     /translation="NLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSK
                     HANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVT
                     GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYV
                     RRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAE
                     GVLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEP
                     KKKEYAVFFI"
BASE COUNT          191 a          303 c          212 g          189 t
ORIGIN      
        1 agcggttcgt gatggttaca gtgtgaacag ggttatatga aaaaaaccgc atattctttc
       61 tttttcggtt ccgggttaag ggacatcagg gagaccgcat tgaacagcgc catcagcggg
      121 tcaatttttc cccgtccact ggcctgtttg gtgataagaa tggcgttacc tttaggctcc
      181 acccgggcat tgccgacaca ccaggccatc aggggctggt caccatgcac cagcacccct
      241 tcagccagtt tgcgctcggt ggttttaatg gccccgccca gtttccagcc ctggcttatc
      301 cccaccacaa ttccgtcggg gatcccggct tccgccagtg aatccagaat ctgccccacc
      361 cctgacgggt caataccgat atggtccagt aactcagcct catgaatgcg acgcacatat
      421 tccgccactt ccgccgtgtc atccccgaca cgccggacaa tggtcatatc tccacaggca
      481 acaagatcct gaaaccggga cgcctcgctc ttccgtcgga ccaccgcggt ttcatgcgcc
      541 caggcatggc cccagcccag ccattcgcgg gtctcccggt cacgcccagt cacatacatc
      601 cccagcagat catccagccc tccgccgtca atccccaccg tcaccacatc agcacgacgc
      661 aggatatcgt ccaggctgat acaacggccc tgctcttccc agaaatcagc ccccgcccag
      721 cggtcagagc gcagggcaag accaatttcc acattggcgt gttttgacat gaacccccgg
      781 aatgtctctt caccggcttc ccgggcttta cggtactccc ggtacagaaa ggcctcatcc
      841 actgaatagc cgagattcgg attgaccatg gcgaggtttt ccatcagcag gttag
//
LOCUS       DAZFDP010000499          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFDP010000499 DAZFDP010000000
VERSION     DAZFDP010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(93..613)
                     /locus_tag="ACHJ1G_005752"
                     /pseudo
     CDS             complement(93..613)
                     /locus_tag="ACHJ1G_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            657..737
                     /locus_tag="ACHJ1G_005753"
                     /pseudo
     CDS             657..737
                     /locus_tag="ACHJ1G_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          257 a          169 c          195 g          273 t
ORIGIN      
        1 tgtaaacata ataataagta ttatgacgtt aaaatttaac catacggtaa aatgattcag
       61 gaaaaccggt tcagcatagc ctaggctgaa cttttgagga tatcaacgaa cggaattttt
      121 gaacatttat gagtagatcg ttgttgaagg gcttcaatga gcatgtcaag ttcatcaact
      181 gctggtctac gattacgacg gtttgattta ccaatcaaag caagtttaag tagatatgga
      241 cgagcacttt tcgccgggtt tgatgtgtaa ttaattccgt atataggttt ggctgcatcc
      301 agaacactgc caagataact aacatcgtga ctgactgttg ctggacctgc accagcgttg
      361 tttctcagcc tgcaatgttc aattacgtca ttttctgtca gttcagatag tttgatcgcg
      421 gagatgtcac tatccataag cagttctagc acatatcttt tagtacggtc tgctttacct
      481 ccggcatttg gtcatttaaa tatttgtgta gtaagtcacg gactgtaagt ccgtcaacag
      541 catttgatga tgaaatgcca tatagatcta ataccatcac tttctgtgtg ccccatgttt
      601 tagcatgagc atgaacgcgg cggctatctc acgggtgatc atccctttgg catactgaaa
      661 agtatcgggt gttactgaac cgtgttgata catcaactgc actggatatt gagtggccga
      721 taataccgac gttataacct ataaatgcca atcaggctga atgttgaaag gatagaatga
      781 acagcccgac acacaaaaaa cggagtccgg ctccggtttt tgtgttgcca tataaggcag
      841 atgtttgtta cagctattta agtctggagt tcaaattaaa atagggagtt ttgt
//
LOCUS       DAZFDP010000500          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFDP010000500 DAZFDP010000000
VERSION     DAZFDP010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>886
                     /locus_tag="ACHJ1G_005754"
     rRNA            <1..>886
                     /locus_tag="ACHJ1G_005754"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          209 a          202 c          279 g          196 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
      301 tttgatgttc taacgttgac ccgtaatccg ggttgcggac agtgtctggt gggtagtttg
      361 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
      421 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
      481 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
      541 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
      601 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
      661 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
      721 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
      781 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
      841 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgcc
//
LOCUS       DAZFDP010000501          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFDP010000501 DAZFDP010000000
VERSION     DAZFDP010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..94)
                     /locus_tag="ACHJ1G_005755"
     CDS             complement(<1..94)
                     /locus_tag="ACHJ1G_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767348.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYI"
     gene            complement(170..>886)
                     /locus_tag="ACHJ1G_005756"
     CDS             complement(170..>886)
                     /locus_tag="ACHJ1G_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767349.1"
                     /translation="QQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNISALNKV
                     MKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGAVGSVAGGILF
                     SPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFL
                     LTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDA
                     SHLGNMVNRLEEALPPELPA"
BASE COUNT          218 a          245 c          218 g          205 t
ORIGIN      
        1 tgatgtaacg ttgatcctgt gcccggagtg gggcagacca cgttaacccc agaatgaagg
       61 cggtaatcat gcaggttttg aacaggtgaa tcatggtatt tacctctctg agtcatgacg
      121 atgacactga caaatcaggt gataaagcgt aaaaggcgca gaatagccgt tatgccggta
      181 actccggggg taatgcttct tccagtcggt taaccatatt gccgagatgg gatgcatcat
      241 attccatgac ggggcgttgc ctgatgatac tgaccaccag tggtttgatt aacatattgg
      301 tcgcggcccg ttgttgtata ccggcggcga aaatgatctc cagagcattc tgatcgataa
      361 cacgggccag attctcgttc agattttcaa tctcatgtcc ggtcagcagg aaactgccgg
      421 caagccatgt cacctgctcc tgaatcattg ccagaatttt gacgcgatct tcttcaacca
      481 gcgctccggc aatttttcct gatacagcgg aggcaatcat tccccccaga acaccgccaa
      541 ccacacgccc tgtcagtgca ccaaatggtc ccagtggact gaacagtatt ccgcccgcca
      601 ctgacccgac agcaccacct gccacaccgg aagatgccac ggcaagattc ctgatgaact
      661 gcgtaccgga gatccgtccc cgcaacattt tgatcatgtc agggccggtt gtgacagcta
      721 ccagtgccag agatgtcacc agcgtaccct tcatcacttt gttcagggca ctgatatttc
      781 ctgcaccggt ccccttctga agagccagtc gaacagtcgg gctggcagtc gaaaaatcaa
      841 tgtgcttcag cataccctga acaacactga gccggtggag ttgctg
//
LOCUS       DAZFDP010000502          881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFDP010000502 DAZFDP010000000
VERSION     DAZFDP010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..860
                     /locus_tag="ACHJ1G_005757"
     CDS             75..860
                     /locus_tag="ACHJ1G_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767350.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          288 a          179 c          194 g          220 t
ORIGIN      
        1 tcaagtggag tatataatta aaaatcgtta gctaaacatt accatgatta tgacaggcga
       61 gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta ttctcgctgc
      121 ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac
      181 aaagtaatat taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg
      241 aaaccactct ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc
      301 gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca
      361 ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca
      421 ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca
      481 gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa gggcatcaga
      541 aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct gcgctgaaat
      601 caacagtatg gtccgactat aaaaatgaaa aactcaccga cgctatctcc ctgcgctacc
      661 tggtacgttt cacgctggtg gatgtggcaa caggtgagtg ggctacctgg tcgccggtga
      721 attatgaata taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata
      781 tgacagagca gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt
      841 tggttaaccg ctatcaataa taaattatat ctgccgccag g
//
LOCUS       DAZFDP010000503          880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFDP010000503 DAZFDP010000000
VERSION     DAZFDP010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..135
                     /locus_tag="ACHJ1G_005758"
     CDS             <1..135
                     /locus_tag="ACHJ1G_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767351.1"
                     /translation="FTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG
                     "
     gene            132..>880
                     /locus_tag="ACHJ1G_005759"
     CDS             132..>880
                     /locus_tag="ACHJ1G_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4767352.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDP"
BASE COUNT          203 a          236 c          271 g          170 t
ORIGIN      
        1 ttcacgcacc acaaaaaagg ggatgccggt aaaaaatcgg caaaaggtga cgcggcgaaa
       61 aaagcggcta accgttttgc ggtgccacca ccaccccgcc tggtggtgaa taatgataat
      121 gagggcaacg gatgatacct gtgtggagca cggcctgccc ggactgggca gagcgcctga
      181 aaaaggggct gtcgattatt ccggctccga tttatccgga gcaggccgca catgccctgg
      241 cgatttttaa acaactgcgg attgtggatg caccgggcag cccgacgttc ggggagtcct
      301 gcgcacagtg ggtgtttgac ctggtggcgg ccctgtttgg ctcctacgat gcgcagaccg
      361 gtgtacgcca tatcaaggaa gtttttatcc ttatccccaa gaaaaacagc aagtccacgc
      421 tggctgccgg gatcatgatg acggcgctgt tactgaactg gcggcaggcg gcgggctaca
      481 ccattctggc cccgaccgtg gaggtggcgg ctaacgcctt caaccctgcc agggatatgg
      541 tacgacggga cgatgatctg gatgacctct gtcaggtgca gacacatatc cggaccatca
      601 cccacagggt gacggacacc acccttaagg tggtggctgc cgatccgaat acggtatccg
      661 gtatcaagtc cgtggggacg ctgattgatg aactgtggtt atttggcaag cagtacaaag
      721 cggaggacat gctacgtgaa gccataggcg gccttgcctc ccgcccggaa gggtttgtgg
      781 tgtatacgac cacccagtcg aatgagccgc cagccggggt gttcagacag aaactgcagt
      841 acgcccggga tgtccgtgac ggcaaaattc atgatccgca
//
LOCUS       DAZFDP010000504          879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFDP010000504 DAZFDP010000000
VERSION     DAZFDP010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>879)
                     /locus_tag="ACHJ1G_005760"
     CDS             complement(<1..>879)
                     /locus_tag="ACHJ1G_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4767353.1"
                     /translation="GLGRDRRIAITEAIQANIELLSGNRRESLIDRIKGADAITLHAW
                     LDRYETILSERGIRPKTLLDYASKIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKA
                     ASAKLIRSTLVDVFREAIAEGHVATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPL
                     PIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNI
                     SLADTLQKCREASGSETIIASTHHEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELR
                     SLSARLYRNQIGDKFAQ"
BASE COUNT          176 a          221 c          209 g          273 t
ORIGIN      
        1 ttgagcaaac ttatcgccaa tctgattccg gtatagcctc gccgacaggc tgcgcagttc
       61 atggaatgtt ggcgggtctc catcaaatga gagtccagat gcatttctcg cctttgtaaa
      121 atacttagat actgttttcg gggaaagcgg ttcgtgatgc gttgatgcaa ttattgtttc
      181 actgccgctg gcctccctgc atttctgtag tgtatcagcc aatgagatat tgagcgcgtc
      241 aatcgttagc gtcagcggaa tggcgagctt agcccctgtt ttaccctgct caatgtgaag
      301 atggttgtcg tttatgtctg accatttcat tctgcataaa tcgcctactc tctgccctgt
      361 aacgacagcc aaatccattg ccagcctcag ccagattggg agcggttcgg ctgcatggta
      421 aatcgcgaca tattcattag ctgtcagtct tgaacgcctt acttctgatt ttgctgtgcg
      481 ggttgctgtt accggattcg tagccacatg cccctcggct attgcctcac gaaaaacgtc
      541 aacaagggtt gacctgatta attttgcgga agccgcttta ccttctgcta cgtaggtgtt
      601 tagcattgct gccacctctt tcgttgatat gtcagcgagc ggtttgtccg gcaattttct
      661 tcggattgcc ctgattttgc tggcgtagtc gagtagagtt ttcggcctga tacccctctc
      721 gctgaggatt gtttcatatc ggtcaagcca cgcatgaaga gtgattgcgt cagcgccttt
      781 aattctgtct atcagtgact cacgcctgtt cccggatagc aactcaatat tggcctgtat
      841 tgcttcagtg attgctatcc tcctgtctcg gcctaatcc
//
LOCUS       DAZFDP010000505          878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFDP010000505 DAZFDP010000000
VERSION     DAZFDP010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..>878
                     /locus_tag="ACHJ1G_005761"
     CDS             124..>878
                     /locus_tag="ACHJ1G_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4767354.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPI
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPVIAAIHREYKQTWKTLD"
BASE COUNT          265 a          210 c          215 g          188 t
ORIGIN      
        1 acctgaataa cgaaacaccg gctggataac cctccagcca tattaacacc ataccaacgg
       61 attaaaaatg ccagcaatgg cagggattcg ttcaccctga aatctgtaat gaggtaaaac
      121 aaaatgagta aagtctttat ttgcgccgcc attccggacg aacaggcaat aaaggaagaa
      181 ggtgcagtcg ctgtagccac tgccattgaa gccggtgatg aacgtcgcgc ccgcgcaaaa
      241 tttcactggc aattcctgga acattatccg gctgctcagg actgcgctta taaatttctt
      301 gtttgcgagg ataaacccgg tattccccgc cctgccctcg attcctggga tgctgaatat
      361 atgcaggaaa accgctggga tgaggagtct gcttcctttg tcccggttga gactgaatca
      421 gatccgataa acgtcacttt tgacaagctg gcccctgaag tacagaacgc tgtcatggtt
      481 aagttcgaca catgtgaaaa catcaccgtt gatatggtga ttagcgcgca ggaactgttg
      541 caggaagaca tggcaacatt cgacggacat atcgttgaag cgttgatgaa aatgccagaa
      601 gttaacgcca tgtatccgga gcttaagctg catgccatcg ggtgggttaa gcataaatgt
      661 aagccaggtg ccaaatggcc cgaaattcag gcagagatgc gcatctggaa aaaacgtcgc
      721 gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc ccgcgccaga
      781 gccaatcaac agcacactga aaattcaaca ggaaaaatca gcccggtcat tgctgccatt
      841 catcgcgaat acaagcagac atggaaaaca ctggatga
//
LOCUS       DAZFDP010000506          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFDP010000506 DAZFDP010000000
VERSION     DAZFDP010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            205..714
                     /locus_tag="ACHJ1G_005762"
     CDS             205..714
                     /locus_tag="ACHJ1G_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4767355.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            686..>871
                     /locus_tag="ACHJ1G_005763"
     CDS             686..>871
                     /locus_tag="ACHJ1G_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767356.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          251 a          179 c          221 g          220 t
ORIGIN      
        1 caggttacgg ggcggcgacc tcgcgggttt tcgctattta tgaaaatttt ccgggatcca
       61 tgtccggttt ctcttcaagt taactatatg aaaaatataa aaacaggtct tctgtgaacc
      121 ggacatgaac aaaaaacaga catgtaaacc ggacatgacc ggttttgttg tgattgtgag
      181 gtgagagttt ttgcgaggtg aggagtggct acgcagactg aagttgccag gcatttaagt
      241 ctgaccgatc gccagcttcg cagattgcag aaattaccgg gtgccccggt ctcgaataag
      301 cgagggcaac tggatctgga tgcctggcgc gatttttaca tatcgtatct gagaagaagt
      361 aaaaacgatg tgcctgatgg cgatagcgaa gaagactatg aagagaaatt gcttattgcc
      421 agatgggaac tgacagcaga acaggctgtt acacagcagt taaaaaatga ggtgtcaaaa
      481 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
      541 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      601 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      661 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      721 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      781 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      841 aacgctgcca ttccagatcg ccatcatgaa c
//
LOCUS       DAZFDP010000507          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFDP010000507 DAZFDP010000000
VERSION     DAZFDP010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..359)
                     /gene="tccP2"
                     /locus_tag="ACHJ1G_005764"
     CDS             complement(<1..359)
                     /gene="tccP2"
                     /locus_tag="ACHJ1G_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO4767357.1"
                     /translation="MINSINSFFSSLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFS
                     PSNSPASATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPLPDVAQRLVQHLAEHG
                     IQPARNMAEHIPPAPNWP"
     gene            complement(675..>867)
                     /locus_tag="ACHJ1G_005765"
     CDS             complement(675..>867)
                     /locus_tag="ACHJ1G_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFO4767358.1"
                     /translation="LSVMSGTSGSSSDAALATRYAAEYFCKTWTAPGLNQAEGYKAIS
                     DLSHHYFRAEGSSPPQSFL"
BASE COUNT          247 a          143 c          221 g          256 t
ORIGIN      
        1 ggccagttag gtgccggagg aatatgttca gccatattcc gggctggttg aatgccatgc
       61 tctgcaagat gctgcaccag acgctgagcc acatcaggta gaggccgatg taaagcgttt
      121 ttttctatac ttgactgagt ataagatgtg ggacgttgaa gtcctgattc tgtataagaa
      181 ttttttacct taaaaatagt tgttgcactg gcaggactgt tgctgggaga aaaaggtgag
      241 ctggttttta ccgtgttagg tgtgcttttg tgctgcgaga cagtgaaggt actgctgcgt
      301 atagcacttg atatactgcg aggaaggctg gaaaaaaaag aattaatgct atttatcata
      361 tagataacct tataagagct ttattggata gtcttattta tccgtttaca tgaaatctac
      421 cttttgtgaa attctcgtca atattttact caaaatgatc ggctgtagga ataaactatt
      481 tgatattatt acgtttttag ggttgttttc aatcagctat agatagggct tggcgccaga
      541 atggagaata tttaacgagg aatagaagct ttgtatcaga cgggcttccc taaatagaga
      601 agttaaataa tcaggatgat gctaatgaat atcagggaca aggtgagtgt cttcatgttc
      661 agtgaaacag ggtgtcacaa aaaagattgg ggggggctag agccttctgc acgaaaatag
      721 tgatgcgaca gatctgagat agctttgtat ccttctgcct ggttcaggcc tggagcagtc
      781 catgttttac aaaaatactc ggcagcgtaa cgtgttgcaa gagcggcatc agacgaggaa
      841 cctgaggttc cgctcatgac cgataat
//
LOCUS       DAZFDP010000508          859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFDP010000508 DAZFDP010000000
VERSION     DAZFDP010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..405
                     /locus_tag="ACHJ1G_005766"
     CDS             <1..405
                     /locus_tag="ACHJ1G_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4767359.1"
                     /translation="KARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLML
                     LVNRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDT
                     GFQQVLERADYLAKEHTKVHCTGERNFGCSRG"
     gene            571..>859
                     /locus_tag="ACHJ1G_005767"
     CDS             571..>859
                     /locus_tag="ACHJ1G_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4767360.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKAS"
BASE COUNT          197 a          238 c          242 g          182 t
ORIGIN      
        1 aaagcccgaa tcgacgctta ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc
       61 acaaccctgc attacgtctg ggcccgggag tttggggagt gcaaaggtaa aaaacactat
      121 cacctgatgc tgctggtcaa ccgggatacc tggtgtcgtg ccggtgatta ccgcgctccg
      181 ggatcactgg ccgggatgat taaacaggcg tggtgcagtg ccctgggagt ggatgtcggg
      241 tgccatgcca cgctggtgca ttttccggcc tggccggcgg tgtggctggc gcgtaatgat
      301 gacaccggct ttcagcaagt gctggaacgt gctgactatc tggcgaagga gcataccaaa
      361 gttcactgca ccggtgagcg caactttggc tgcagtcgtg gctgagccag acagacgagt
      421 tatctgactt cagtgtgatg acgtgttcac ggccaccaca ctgattttcc cttatgagac
      481 tgaactgaaa ccgccattca caccggacca cgctgcccgt accgggacgg ctgttgcctg
      541 cgtgtcgtct gacggtaagg aacatatact atgtacgcaa aatcctttct cgctcttgat
      601 ggcaacggac gtctgacggg cgcccgtaca gcacaggccg caccttatgc tcactacacc
      661 tgccacttgt gtggcagtgc actcagatac catccgcaat acgacactga acttccctgg
      721 tttgaacaca ctgacgacag gctgacagag cacggtcaac agtgccctta tgtcaggccg
      781 gagcgcagag aaatacagtt gattaaacgt ctgcagcaat tcgtaccgga tgccttaccc
      841 gtggtgcgta aagccagct
//
LOCUS       DAZFDP010000509          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFDP010000509 DAZFDP010000000
VERSION     DAZFDP010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>846
                     /locus_tag="ACHJ1G_005768"
     rRNA            <1..>846
                     /locus_tag="ACHJ1G_005768"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          239 a          187 c          261 g          159 t
ORIGIN      
        1 ggaaaggcgc gcgatacagg gtgacagccc cgtacacaaa aatgcacatg ctgtgagctc
       61 gatgagtagg gcgggacacg tggtatcctg tctgaatatg gggggaccat cctccaaggc
      121 taaatactcc tgactgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc
      181 cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg gagcacgctt
      241 aggcgtgtga ctgcgtacct tttgtataat gggtcagcga cttatattct gtagcaaggt
      301 taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag ttgcagggta
      361 tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa cactaactgg
      421 aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc
      481 caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg cctcgtgaat
      541 tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta ccaacccgat
      601 gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg ctaacgtccg
      661 tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg gttaagtggg
      721 aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc atcatttaaa
      781 gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc
      841 atgcac
//
LOCUS       DAZFDP010000510          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFDP010000510 DAZFDP010000000
VERSION     DAZFDP010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..757
                     /gene="pagP"
                     /locus_tag="ACHJ1G_005769"
     CDS             197..757
                     /gene="pagP"
                     /locus_tag="ACHJ1G_005769"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4767361.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          227 a          156 c          184 g          247 t
ORIGIN      
        1 tttctttttt gactattccc atcgcagaaa acgacgcatc atctttaatc gatgcgcgga
       61 aatatttaac ttgaacaagc ggaaataaat agagcagcta ttcagattat tctttatgtt
      121 gggtctatta aggttatgtt aattgtagct ttgctatgct agtagtagat ttttgataaa
      181 tgttttatgg tcacaaatga acgtgagtaa atatgtcgct atcttttcct ttgtttttat
      241 tcagttaatc agcgttggta aagtttttgc taacgcagat gagtggatga caacgtttag
      301 agaaaatatt gcacaaacct ggcaacagcc tgaacattat gatttatata ttcctgccat
      361 cacctggcat gcacgtttcg cttacgacaa agaaaaaacc gatcgctata acgagcgacc
      421 gtggggtggc ggttttggcc tgtcgcgttg ggatgaaaaa ggaaactggc atggcctgta
      481 tgccatggca tttaaggact cgtggaacaa atgggaaccg attgccggat acggatggga
      541 aagtacctgg cgaccgctgg cggatgaaaa ttttcattta ggtctgggat tcaccgctgg
      601 cgtaacggca cgcgataact ggaattacat ccctctcccg gttctactgc cattggcctc
      661 cgtgggttat ggcccagtga cttttcagat gacctacatt ccgggtacct acaacaatgg
      721 caatgtgtac tttgcctgga tgcgctttca gttttgagac aaatgaagtt ttagtaactt
      781 ctttaaaatc aatagctaaa ataagtaaca tcaa
//
LOCUS       DAZFDP010000511          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFDP010000511 DAZFDP010000000
VERSION     DAZFDP010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHJ1G_005770"
     CDS             <1..224
                     /locus_tag="ACHJ1G_005770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767362.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHJ1G_005771"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHJ1G_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            673..>813
                     /locus_tag="ACHJ1G_005772"
     CDS             673..>813
                     /locus_tag="ACHJ1G_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4767363.1"
                     /translation="MAIPYEPYGDITMTYKYNPFWQQRIRETVRHALNVHPRLTALRV
                     DLR"
BASE COUNT          214 a          211 c          204 g          184 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgt
//
LOCUS       DAZFDP010000512          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFDP010000512 DAZFDP010000000
VERSION     DAZFDP010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>806
                     /locus_tag="ACHJ1G_005773"
     CDS             <1..>806
                     /locus_tag="ACHJ1G_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4767364.1"
                     /translation="AAGVTDANYYPEGMHSQKRENWREYLERERGKKPGEEKHHQRKT
                     TLPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGG
                     RRVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIA
                     ARQVERETGKPVRMIILDTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHH
                     SGKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          191 a          179 c          273 g          163 t
ORIGIN      
        1 gcggcgggtg taactgatgc gaattattac cccgaaggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgg gggaaaaagc caggggaaga aaaacaccac
      121 cagcgaaaaa ccacgctacc aatgagcgtg ggatctgccg gatacgacac acaacttgat
      181 tacgtggtta aggggattat tccggcggta tcgctgtgca gcatatacgg ggcgagcggt
      241 tcatataaat cgttccttgc gggttcgtgg gcgtgtcacg tttccacggg ccgccagtgg
      301 ggaggccgca gggtggcgca tggcgctgtt ctctatgtgg ttggtgaagg cggtatcggc
      361 gttccgcgtc gtgtaaaagc ctgggaggtt gtgcacggtg agcaggtgaa aaatctgtat
      421 ctggtaaacc gtcccatctt tccggctgcc ccgcttgatg ttgaggaaat ggttatcgct
      481 gcccgtcagg tggaacggga aacgggtaaa ccggttcgaa tgattattct ggatacgctg
      541 gcgcgctgct ttggcggcaa tgatgaaaat gattcccgtg atatgggggc gtttatccgt
      601 gggtgtgacg aactgaaacg gcgcacaggg gcaacggtgc tggtggttca ccattccggc
      661 aaggatgaaa caaagggggc gcgtggttcc agtgcatttc gtgcatcact ggacgccgaa
      721 taccggatac gcagggagga cgcaggcagc gaagcgctgg ttatctcatg taccaaaatg
      781 aaggacgcgg aggaactcaa agaagc
//
LOCUS       DAZFDP010000513          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFDP010000513 DAZFDP010000000
VERSION     DAZFDP010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>803
                     /locus_tag="ACHJ1G_005774"
     CDS             <1..>803
                     /locus_tag="ACHJ1G_005774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4767365.1"
                     /translation="AAGVQDANYYPDGMHSQKRENWREYLERERGEKQEEKGHQRKTK
                     LPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGR
                     RVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAA
                     RQVERETGKPVRMIILDTLARCFGGNDENDSRDMGTFIRGCDELKRRTGATVLVVHHS
                     GKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          184 a          181 c          270 g          168 t
ORIGIN      
        1 gcggcaggtg tacaggatgc gaattactac cccgatggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgc ggggaaaagc aggaagaaaa aggccaccag
      121 cgcaaaacaa agctaccgat gagcgttggt tctgccggat atgacacgca actggattac
      181 gtggttaagg ggattattcc ggcggtatcg ctttgcagca tatacggggc gagcggttcc
      241 tataaatcgt tccttgcggg ttcgtgggcg tgtcacgttt ccacgggccg ccagtgggga
      301 ggccgcaggg tggcgcatgg cgctgttctc tatgtggttg gtgaaggcgg tatcggcgtt
      361 ccgcgtcgtg taaaagcctg ggaggttgtg cacggtgagc aggtgaaaaa tctttacctt
      421 gtaaaccgtc ccatctttcc ggctgccccg cttgatgttg atgaaatggt tatcgctgcc
      481 cgtcaggtgg agcgggaaac gggtaaacct gttcgaatga ttattctgga tacgctggcg
      541 cgctgctttg gcggcaatga tgaaaatgac tcccgtgata tggggacgtt tatccgtggg
      601 tgtgacgaac tgaaacgacg cacaggggcc acggtgctgg tggttcacca ttccggcaag
      661 gatgagacga aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga tgctgaatac
      721 cggatacgca gggaggacgc aggaagcgaa gcgctggtta tctcatgcac caaaatgaag
      781 gacgcggagg aactcaaaga agc
//
LOCUS       DAZFDP010000514          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFDP010000514 DAZFDP010000000
VERSION     DAZFDP010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..802
                     /locus_tag="ACHJ1G_005775"
                     /pseudo
     CDS             1..802
                     /locus_tag="ACHJ1G_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          257 a          137 c          188 g          220 t
ORIGIN      
        1 cataaattat cggatattct actgttgact atttgtgccg ttatttctgg tgcagaaggc
       61 tgggaagata tagaggattt tggggaaaca catcccgatg ttttgaagtg atatggtgat
      121 tttgaaaatg gtattcctgt tcacgatatc attgccagag ttgtatcctg tatcagtcct
      181 gcaaaatttc acgagtgctt tattaactgg atgcgtgact gccattcttc agatgataaa
      241 gacgtcattg caattgatgg aaaaacgctc cggcactctt atgacaagag tcgtcgcagg
      301 ggagcgattc atgtcattag tgcgttctca acaatgcata gtctggtcct cggacagatc
      361 aagacggatg agaaatccaa tgagattaca gccattcctg aacttcttaa catgatggat
      421 attaaaggaa aaatcatcac aactgatgcg atgggctgcc agaaagatat tgcagaaaag
      481 atacaaaaac agggaggtga ttatttattc gcggtaaaag gaaaccaggg gcggctaaat
      541 aaagcctttg aggaaaaatt tccgctgaaa gaattaaata atccagagca tgacagttac
      601 gcaatgagtg aaaagagtca cggcagagaa gaaatccgtc ttcatattgt ttgcgatgtc
      661 cctgatgaac ttattgattt cacgtttgaa tggaaagggc tgaagaaatt atgcgtggca
      721 gtctcctttc ggtccataat agcagaacaa aagaaagagc cagaaatgac ggtcagatat
      781 tatatcagtt ctgctgattt aa
//
LOCUS       DAZFDP010000515          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFDP010000515 DAZFDP010000000
VERSION     DAZFDP010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..514
                     /locus_tag="ACHJ1G_005776"
     CDS             11..514
                     /locus_tag="ACHJ1G_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4767366.1"
                     /translation="MTGYVVRWDKLSELLWGEFYEKFQRGAFTEWLAAGNDVRGLYEH
                     DHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTGRDVIELVKRGDISGMSFGFRSRK
                     DVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARRSLYEQHPEKMPRADNRRW
                     WADLAGV"
     gene            516..>799
                     /locus_tag="ACHJ1G_005777"
     CDS             516..>799
                     /locus_tag="ACHJ1G_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767367.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGSGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQT"
BASE COUNT          175 a          203 c          253 g          168 t
ORIGIN      
        1 agccggtacg ctgacgggct acgttgttcg ctgggataag ctttcagaac tgctatgggg
       61 ggagttttac gaaaaattcc agcggggggc gtttactgag tggcttgcgg cgggtaatga
      121 cgttcgcggc ctgtatgagc atgaccacag catgttactg gggcgcaccc gttccggcac
      181 gctgaaactg gaagaggacg acacagggtt acgctttgaa cttaccccac cggataccag
      241 cacagggcgt gacgttatcg aactggttaa acgtggtgat atatccggca tgagctttgg
      301 ctttcgttcc cgtaaggatg tatgggatac cacaacagat ccatgcgtgc gcaccgtgct
      361 ggtggcggaa ctgtacgaaa ttaccgttac atccgtaccg gcttaccctg attccggcgt
      421 ggagctggcc cgccgttccc tgtatgagca gcaccccgaa aaaatgccgc gtgcggataa
      481 tcgccgctgg tgggcggatt tagcgggggt gtgatatgtg gcctttcaga agaaaaaaag
      541 agcagcgcag catgacgctg gatgaattta tggcgctggc cggcacatcg aacacggggt
      601 cgggtgagta cgtatcatcg gggacagcgg aatcactgcc cgccgtcatg aacgccgtca
      661 cggtcatctc tgaggcggtg gctaccatgc cgtgttacct gtacctggta cgcaatgaga
      721 aggggaagga ggcccgagag tggcttgatt ctcatccggt cgatcacatc ctcaacgagc
      781 gcccgaacgc gtggcagac
//
LOCUS       DAZFDP010000516          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFDP010000516 DAZFDP010000000
VERSION     DAZFDP010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..>795
                     /locus_tag="ACHJ1G_005778"
     CDS             30..>795
                     /locus_tag="ACHJ1G_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767368.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEV"
BASE COUNT          264 a          155 c          156 g          220 t
ORIGIN      
        1 atttcgattg atttagtgtt tattgacgta tgtactggat tattaacgat aatatcgagt
       61 tctggcctga gcaccgaaaa ttaatatcgg tacataacgc cgatcttaac gtcgttctga
      121 caacgccagc cagtcgatgt ttatcacttc tacttgaagc ttttcctgat gtggttgcac
      181 aacaagattt tttcaccaga gtctgggaag aagaaggtat gcgtgtgcct actaacacgt
      241 tatatcagaa catatccatt atcagacgcg gatttcgcgc tgttggtgat actacccact
      301 cgctaattgc aaccgtgccg agaagaggat tcaagatcca taatgacatt aacatacaaa
      361 atcatgtaat aaactcgtca acagacgcac atacacacaa tgccccacct gccataaaag
      421 ttaatgcggg atacaaagag agcattggtg gcgcaaagaa tttcaataac aaaatcctca
      481 aacatataaa aagtcatcta attatgttga gcgcatttgt cataggcgca tattctgcat
      541 attggctatg gaataataat caaccgaagc catttttcaa ggattacaag actgttgcgg
      601 aaattaatgg ctgccatttt aatgtaacag aagatacaat tgacgggttg aaagagttcg
      661 ataaatataa aacacggata ctggattcgg ggattaactg taaaaaacat ccgtggttat
      721 acttccctct tgcaaaatcc tcaccgggga tgattgttat ggcatgcaat aaaaattata
      781 accaacatga agtgg
//
LOCUS       DAZFDP010000517          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFDP010000517 DAZFDP010000000
VERSION     DAZFDP010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..>790
                     /locus_tag="ACHJ1G_005779"
     CDS             103..>790
                     /locus_tag="ACHJ1G_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4767369.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          263 a          133 c          171 g          223 t
ORIGIN      
        1 ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca catgttaacg
       61 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
      121 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      181 ggtgtagaaa tctatgctgc tgccaaacaa ggtgaacccg atccagaatt aaacacatct
      241 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      301 gcaattgata aagccaaagg cggcggagat gaaacgttcg tacagggacg ttatgaaggc
      361 tttggtccta atggctcaat gattattgct gagacattga cttcaaatgt taaccgtacg
      421 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      481 gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggaacaga ccctgaccat
      541 atttttgaaa ttttgcttga agctgaagtt gatgttcgtg atgtaactga agaagaaggt
      601 aatattgtta tttatactga acctactgac cttcataaag gaatcgcggc gctaaaagca
      661 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      721 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      781 gatgtacaaa
//
LOCUS       DAZFDP010000518          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFDP010000518 DAZFDP010000000
VERSION     DAZFDP010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..319)
                     /gene="escT"
                     /locus_tag="ACHJ1G_005780"
     CDS             complement(<1..319)
                     /gene="escT"
                     /locus_tag="ACHJ1G_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO4767370.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGS"
     gene            complement(319..588)
                     /gene="escS"
                     /locus_tag="ACHJ1G_005781"
     CDS             complement(319..588)
                     /gene="escS"
                     /locus_tag="ACHJ1G_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO4767371.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(588..>790)
                     /gene="escR"
                     /locus_tag="ACHJ1G_005782"
     CDS             complement(588..>790)
                     /gene="escR"
                     /locus_tag="ACHJ1G_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO4767372.1"
                     /translation="QLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPF
                     KILLFILVGGWQKLFEFLLVVN"
BASE COUNT          289 a          155 c          131 g          215 t
ORIGIN      
        1 ttgaacctct taaagtatca ataatctgcc cagccgcatc tattgcccaa aaaagaatag
       61 taaatgataa cccaatgaaa aagcctatac ttatttcttt tagcactatg cctgtcaact
      121 gaaaaattcc ggatggtaat ttttccgaaa atgtatagtt ctctactata attggaagtg
      181 taaacgcaat cataattgag ttgcgtataa agttactgag caaaacacct gttgaaaata
      241 ttggtagaat aataaacatc cccagcggcc tgagtataca aaaaaatgat gatactatga
      301 ccgtcattat ttcattcatt agccattcac cttcggaatc atttcaaata ttatcgacga
      361 gaagttgatg attgttgttc ccatccagtg ataagtcagg gcaagcgtag caaacactgc
      421 tattattttt agcaaaaaag gcaatgtttg atcctgtaac tgcgttatag cctgaaccag
      481 gctaataata atgccgacaa cagaggccgc tatgactgta ggcagtgaga ggataaatat
      541 tatccaaagc gtttgcacac ataattgaac aaaatatcca gtttccatca attcaccacc
      601 aacagaaatt cgaataattt ttgccagcct ccaacaagaa tgaataacaa gatcttaaaa
      661 ggaatcgaaa ttgttactgg cgataccatc atcataccca aggccaataa gatattggaa
      721 atgatcaaat ctatcgcaat aaaaggtaaa tagagcaaaa aacctatctt gaatgcagct
      781 tcaagctgcc
//
LOCUS       DAZFDP010000519          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFDP010000519 DAZFDP010000000
VERSION     DAZFDP010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>783
                     /locus_tag="ACHJ1G_005783"
     CDS             <1..>783
                     /locus_tag="ACHJ1G_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFO4767373.1"
                     /translation="NMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISG
                     PKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQ
                     CQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLE
                     LTLLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIM
                     NHPLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGD"
BASE COUNT          235 a          174 c          147 g          227 t
ORIGIN      
        1 aaaatatgca cagtgagatc cgtgcatacc acgaagatgc aggttttttc gcggcctggg
       61 attttgcttc aaattatcgt gatggggaat catatgtttt actcgctgaa aaaatgacaa
      121 ctgtgatcag cgggcctaaa actacgatga cgctgattta tcactacaat tcctcttatc
      181 ttcgcttcgc atttaccctg acacaggaca gtccacgcgt tgatgtccaa acttttattg
      241 attggcacga acctaatgtt tctctcaaag taaaattccc ggtatcagta caaacatctc
      301 ttgcacaatg tcaaattcag ttcggtgtaa ttgacagacc aacgcatagc gatgattcgt
      361 tcgcttttgc caaagatgaa ataccagcac atcactgggt cgacctttct gatcaagaaa
      421 ctggcattgc tttaatagca aaaaacaaat atggctatcg tgtcaaagac cagacgctag
      481 agttaacttt gttgcgttcg cagcacaaac ctggggaagt tgttaagaca gataattatg
      541 ataatttcct gattaataat tttgcagata ttggtaaaca aaaattcaca ttctccttat
      601 ttcctcatca tggagattac cgtgtgggtg gtgtcgttaa ccaggcctat ataatgaatc
      661 atcccttaca gatcgtccct gtcaaaccac acccgggtga actaccccct tcactatcat
      721 tttttgctat cgatactaac agcgttatca tcgaaacaat aaaattagct gaagacggcg
      781 acg
//
LOCUS       DAZFDP010000520          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFDP010000520 DAZFDP010000000
VERSION     DAZFDP010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>782)
                     /locus_tag="ACHJ1G_005784"
     CDS             complement(<1..>782)
                     /locus_tag="ACHJ1G_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767374.1"
                     /translation="AKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFFELW
                     LNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITPDRNCGITES
                     VTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGVIE
                     VPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKMTA
                     EIVCSVFRVPAYKIGVGHPPSSDNVEALEQQYYSQCLQTLIES"
BASE COUNT          162 a          241 c          196 g          183 t
ORIGIN      
        1 gactcaatca gcgtctgcag acactgggaa taatactgct gctccagcgc ctccacgttg
       61 tcactggaag gcgggtggcc aacgccaatc ttgtaggccg ggacacggaa caccgaacag
      121 acaatttcag ccgtcatttt cagttgttcc accgtctgcg catccaccgg tgaaaacgtc
      181 gtggggctgt attttgcccc gttgctcagt atggccgtct tcccggcatt ttcgcccgta
      241 tatccgctgt cccagttccc cttcagtttt ttcgcgtttt cttccgtaat actgccgggg
      301 acctcaatca cgcctgatgg tcgcccgcca tttctgaaaa aatacgtcga atttgcctga
      361 atatgatgcc cctgcatggc ggccagtcct gcggcataca ccggcggcag ccccacaagc
      421 ggatgaaaaa aacagttaaa acggtcgtgg atcacctccc gggcaggcac cgtcaccgac
      481 tctgtgatcc cacagttccg gtccggcgtg atgcggtaga acacgtcgcc gtcatccgcc
      541 accagaggtt caacccggtt ccagtccaga atacgcagtt ctttgatctg cccccggggg
      601 ttgcggattt tcagcaccac cgtattgccg tgacgcaatt tggaattcag ccacagttca
      661 aaaaactgga tgcgattctg ctgcgcatta ggacgacgac agagacgggc agtatctccc
      721 tgccgctttt cccggcgtat tccctgtaca tcagtctgca tcaggcgaag ccgcattttg
      781 gc
//
LOCUS       DAZFDP010000521          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFDP010000521 DAZFDP010000000
VERSION     DAZFDP010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            383..598
                     /gene="essD"
                     /locus_tag="ACHJ1G_005785"
     CDS             383..598
                     /gene="essD"
                     /locus_tag="ACHJ1G_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO4767375.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            598..>781
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005786"
     CDS             598..>781
                     /gene="rrrD"
                     /locus_tag="ACHJ1G_005786"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4767376.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          198 a          147 c          186 g          250 t
ORIGIN      
        1 ttaatggctg ttatttataa gtgccctata atttgaaggt tcaatttaaa tcggctaaaa
       61 ataacgctgg aaattatttg ttggttattt gttgagattt gtttatgtat ttgtagtggt
      121 gttttcaata ctcggtagca ttctcgcaaa tatcatttag tggtttacgt acgtaaaaaa
      181 ttggttatgc tgttaagagt ggttacttcg tcacacagct taaacccgcc gtcgagcggg
      241 tttttccatt ttttgagtct cgatattagc tgataaccca atacctgagt tattcactga
      301 ctccgagtct gttacgtttc tgcttttttg cgatacgttg tattccctca atttacaccc
      361 gctttgtctg cgaggtgggg ttatgaaatc catggataag ttaacaacgg gtgtcgccta
      421 tggcacctca gcaggtagtg ccggttactg gtttttacag ctgctcgata aagtcacgcc
      481 ctcacagtgg gcagcaatag gtgtgctggg tagcctggta tttggcctgc tgacgtacct
      541 gacaaacctt tatttcaaga ttaaagaaga taagcgcaag gctgcgagag gtgaataatg
      601 cctccatcat tacgaaaagc cgttgctgct gctattggtg gcggagcaat tgctatagca
      661 tcagtgttaa tcactggccc aagtggtaac gatggtctgg aaggtgtcag ctacatacca
      721 tacaaagata ttgttggtgt atggactgta tgtcacgggc atacaggaaa agacatcatg
      781 c
//
LOCUS       DAZFDP010000522          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFDP010000522 DAZFDP010000000
VERSION     DAZFDP010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            214..576
                     /locus_tag="ACHJ1G_005787"
     CDS             214..576
                     /locus_tag="ACHJ1G_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767377.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            592..720
                     /locus_tag="ACHJ1G_005788"
     CDS             592..720
                     /locus_tag="ACHJ1G_005788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767378.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
BASE COUNT          239 a          155 c          164 g          222 t
ORIGIN      
        1 atagagatca ttattcaatt cgattgagaa aaatcatcat aaggtttata tccatttatt
       61 agattttatg tgtataaata gttaaaaatg cacgatgtag agatatttgt gcttaacatc
      121 aattaatcat tgctaaatcg tgcttattgt tcagaaatcg cccaagtcat attgaaaatc
      181 tgttggcgaa cacctatata aggagtatag ttagtgaccc aaaacgtatt gccaatcaac
      241 aagagcttgc atgaccgagc tgttgatgaa tttaatcgtc tgcatggaac aatgattggc
      301 gagattagtg caatgctgaa aacagctaaa gtagcgccat tggtggatct tcgtaagaaa
      361 gatcctacct tttcgaacgt tgtcgcagaa ttacggacat tcagagacgt atgtaatgct
      421 ctgctcccat actttcgtgt agataagacc agcgaaatcg ctgtcatcga taagttactt
      481 atactggcta atgacctggc tcaagccatc gatgctgacg atcctgacgc attgtgcgca
      541 gcaatagcag cccttgatgt tgagccttac atttaaacga ggggaaaaaa aatgactaaa
      601 gcgtttgact atactgccgt tagtaagctt ttggctgaaa tgcgcggatg tgtagagcgc
      661 gttcaaaatc tacgccgcga ctttgaagct cacatcaatc attcacaaaa agcggcttga
      721 tacttaagtt agatgacaga acccggcctc agcgccgggt tttctttgcc tcacgatccc
//
LOCUS       DAZFDP010000523          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFDP010000523 DAZFDP010000000
VERSION     DAZFDP010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..774
                     /locus_tag="ACHJ1G_005789"
                     /pseudo
     CDS             83..774
                     /locus_tag="ACHJ1G_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          299 a          152 c          118 g          205 t
ORIGIN      
        1 gtgtgtagaa gaaaatccat tcctgttcta attatccaat ttaaacatct tagcataaaa
       61 caaatgatga ataaggaatt ttatgtcaat tgactttacc ccaggtataa taaatacata
      121 tcacggcgat atttataact gcacaacaaa taccgataat gctaaaacgc cagacacacc
      181 gaagtggcct tgcgataatt gggaagaaca ataacccatt aattccacat tctctggaga
      241 aggatatatc tctgatcaat atgatttagc gcaacaccaa cttcaacaaa ttaatgcgtg
      301 ccacacgaac actacatata caaatgcaga ctactcaaaa gttgtggctc aacttgtaag
      361 tcttattact aacattgaaa cgataagttc gacacaactt acgcaacaga cacaatcaat
      421 acttaatcag attaataaca ttagatatga aaaaaacaaa agtgctgaat gtcgaatcat
      481 tgttgtcgct aatcctaaac ccgacaaggc aatcataact aaaattagcg tagaagaggg
      541 aatacccata acattcagtg tccaaaccat gttttcagat actaatttta ttgctgaaca
      601 acgagctgac ttaccaacta atataaaaga tattcaaagc ctgtatcaaa aaatgacgaa
      661 gttatatatt gagcacagtg aaaataaaaa caggatgaaa gtctttgccg gaaccaactt
      721 tattgatttc aatatgacag gacagaacct gtccgggttc gttctaacct tgtc
//
LOCUS       DAZFDP010000524          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFDP010000524 DAZFDP010000000
VERSION     DAZFDP010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>767
                     /gene="escU"
                     /locus_tag="ACHJ1G_005790"
     CDS             <1..>767
                     /gene="escU"
                     /locus_tag="ACHJ1G_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO4767379.1"
                     /translation="VELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVG
                     TVTTGVSQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFY
                     FMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKK
                     MKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICL
                     YYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIE"
BASE COUNT          241 a          122 c          151 g          253 t
ORIGIN      
        1 tgttgagtta gttaatacga ctatagactc gctaaataga ccgtttttgt atgccattcg
       61 tgaaatatta ggtgcggtat taaacatatt tttattgtat attttgccaa tttctttgat
      121 tgtctttgtt ggaactgtta caactggtgt atcacaaaca ggattcatct ttgcggttga
      181 aaaaataaaa ccatcggctc agaagattag tgtaaaaaat aacctgaaaa atattttttc
      241 tgtaaagagt atttttgagc tgcttaagtc agtatttaag ttagtgataa ttactctcat
      301 tttttacttt atggggcatt catatgcaaa tgagtttgca aatttcacaa gactgaacgc
      361 atatcaaact cttgtcgttg ttgccttttt tgtttttctt ttatggaaag gcgtgttatt
      421 cggatatcta ctcttttcag tatttgattt ctggttccag aagcatgagg gactgaagaa
      481 aatgaaaatg agtaaagatg aggtgaaacg agaagccaag gatactgatg gtaaccctga
      541 aattaaaggg gagcgccgtc gccttcattc tgagatacaa agtggaagtc tggctaataa
      601 catcaaaaaa tcaaccgtga tcgtcaaaaa cccgactcac attgcgattt gcctatacta
      661 taaactcggg gagactccat tgcctttagt tattgaaaca ggaaaagatg ccaaagctct
      721 acagatcatt aaactggctg aactctatga tattccagtg attgaag
//
LOCUS       DAZFDP010000525          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFDP010000525 DAZFDP010000000
VERSION     DAZFDP010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>763
                     /locus_tag="ACHJ1G_005791"
     CDS             <1..>763
                     /locus_tag="ACHJ1G_005791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767380.1"
                     /translation="LDREVTLPETGTSTVNLINGSGKPVSVDITAHPAPDRIQVSTLP
                     DGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQ
                     SGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVVADDGSERL
                     VSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPG
                     YFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVET"
BASE COUNT          155 a          215 c          256 g          137 t
ORIGIN      
        1 ctggaccgtg aggtgacact gccggagaca ggtacatcga cggtgaacct gattaacggc
       61 agcggtaagc cggtgagtgt ggacatcacc gcacaccccg cgccggaccg gatacaggtc
      121 agtaccctgc cggatggcgt ggcgacatac ggtgtgtggg gactctccct gccgtcactg
      181 cgtcgtcgcc tgttccgctg tgtttccatc cgggaaaaca cggacggcac ctttgccatc
      241 acggcggtgc agcatgtgcc ggaaaaagaa gccatcgtgg ataacggggc cagctttgag
      301 ccgcagtcag gcaccctgaa cagcgtcatc cctccggcag tgcagcacct gacggtggag
      361 gtgagcgcag ctgacggtca gtatctggcg caggcgaaat gggacacgcc gaaggtggtg
      421 aagggcgtga gctttatgct tcgcctgacc gtggtcgcgg atgacggcag tgagcggctg
      481 gtcagcacgg cccggacgac ggaaaccaca taccgcttca cgcaactggc gccggggaac
      541 tacaggctga cagtccgggc ggtaaatgcg tgggggcagc agggcgatcc ggcgtcggta
      601 tcgttccgga ttgccgcacc ggcagcgccg tcacagattg agctgacacc gggctatttt
      661 cagataacag cggtcccgcg tcttgcggtg tatgacccga cggtacagtt tgagttctgg
      721 ttttcggaaa cgcggattac cgatatcagg caggttgaaa cca
//
LOCUS       DAZFDP010000526          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFDP010000526 DAZFDP010000000
VERSION     DAZFDP010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..284
                     /gene="yddL"
                     /locus_tag="ACHJ1G_005792"
                     /pseudo
     CDS             9..284
                     /gene="yddL"
                     /locus_tag="ACHJ1G_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            323..>760
                     /locus_tag="ACHJ1G_005793"
     CDS             323..>760
                     /locus_tag="ACHJ1G_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4767381.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINF"
BASE COUNT          268 a          153 c          129 g          210 t
ORIGIN      
        1 tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg
       61 tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg
      121 ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc
      181 gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg
      241 aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaag aatctctgat
      301 tgagttaagt gcccatcaca atatgatcac tgaccttata ttacacaatc atcccaggat
      361 gaaaacaatc actttaaacg acaaccatat tgcacattta aacgccaaaa acactacaaa
      421 actggaatat ttaaacttaa gcaataacaa tttactgcca accaatgaca ttgatcaact
      481 aatatcatca aaacatcttt ggcatgtatt agttaacgtc atcaacaatg atccacttgc
      541 acaaatgcag tactggactg cagtaagaaa tataattgat gacactaatg aagtgaccat
      601 tgatttatca ggacttaatt taaccactca accaccaggg ctgcaaaact tcacctctat
      661 caatcttgat aataaccaac tcacacattt tgatgcaacc aactacgata gactcgtaaa
      721 gctaagtctg aatagtaatg ctcttgagtc aataaatttt
//
LOCUS       DAZFDP010000527          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFDP010000527 DAZFDP010000000
VERSION     DAZFDP010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..495
                     /locus_tag="ACHJ1G_005794"
     CDS             61..495
                     /locus_tag="ACHJ1G_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO4767382.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          169 a          168 c          235 g          184 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagt
//
LOCUS       DAZFDP010000528          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFDP010000528 DAZFDP010000000
VERSION     DAZFDP010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>754
                     /gene="ehxD"
                     /locus_tag="ACHJ1G_005795"
     CDS             <1..>754
                     /gene="ehxD"
                     /locus_tag="ACHJ1G_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4767383.1"
                     /translation="ERDAALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQE
                     NSYIQAKNEHAVWLAQVSQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEH
                     ELEKNRQRKASSFIKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLN
                     KDIGFIQPGQEVVIKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRND
                     IQGERKKIPVTAGMTVMAEIKTGVRSVISYLLS"
BASE COUNT          258 a          119 c          172 g          205 t
ORIGIN      
        1 gaacgggatg cagcgcttgc tcgtgttagc ctgtatgagc atcaggtatc acaggaagga
       61 agaaaactca atgattttaa gtatttgttg aataaaaaag ctgtttctca acattcagtt
      121 atggagcagg aaaatagcta tattcaggca aaaaatgaac atgcagtctg gcttgcacag
      181 gtttctcaac ttgaaaaaga aatagaactt gtgcgggaag aactggcact ggagacgaat
      241 atcttcagaa gtgaaattat cgagaagcac agaaaatcaa cagataacat tgtgttgctg
      301 gagcatgagc ttgaaaaaaa caggcagaga aaagcatcat cttttattaa agctcctgtg
      361 agtggtactg ttcaggagtt aaatatacat acagaaggtg gtgttgtcac aacagcagaa
      421 acgctgatga ttattgtccc tgataatgat attctcgaag taacagcatc tgtacttaac
      481 aaggatatcg gttttataca acctggacag gaggtcgtta ttaaagtaga tgcatatcca
      541 tatacacgtc atggttacct tacagggaaa gtaaaaaata taactgcaga ttctgtttct
      601 gttccggata cagggcttgt atttaacgtg attatatctg ttgatcggaa tgatatacag
      661 ggagaaagaa aaaaaatccc tgttacagct ggaatgactg ttatggcaga aataaaaacc
      721 ggggtgcgta gtgttatcag ctatcttctt agtc
//
LOCUS       DAZFDP010000529          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFDP010000529 DAZFDP010000000
VERSION     DAZFDP010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..459)
                     /locus_tag="ACHJ1G_005796"
     CDS             complement(<1..459)
                     /locus_tag="ACHJ1G_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4767384.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
     gene            complement(440..>745)
                     /locus_tag="ACHJ1G_005797"
     CDS             complement(440..>745)
                     /locus_tag="ACHJ1G_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767385.1"
                     /translation="FSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYE
                     KECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          143 a          245 c          204 g          153 t
ORIGIN      
        1 tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gcccccgcca ccatcccgcc
       61 gggcgtgtcc atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag
      121 acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg cccgcgtccg
      181 gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcatgacct gataactgcg
      241 ggcctgtcgt ggtccgtcat catcaccgga taatgccagc gtcgcgagtg cctcctgggc
      301 agtcaggctg tcgccggaca ccgcatccgt caggcggctg atcccaagct ggcctgcaag
      361 cgcacaaaag aaaacccgcg cataggcggg ttcaagcatc agcggctcat taaaagccat
      421 gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt
      481 gtcgcagtcc ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccggctgcgc
      541 ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt
      601 ttgcgcactc tttctcgtag gtactcagtc cggcttctat cagcatcacc gcttcctgta
      661 cttctttcag accatcgatg gccatacgac cggagcctat ccagtcacag ttcccccagg
      721 cgctgcgggc ttcctgaaag ctgaa
//
LOCUS       DAZFDP010000530          737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFDP010000530 DAZFDP010000000
VERSION     DAZFDP010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..321
                     /locus_tag="ACHJ1G_005798"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHJ1G_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2..66
                     /locus_tag="ACHJ1G_005798"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          192 a          137 c          192 g          216 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgcgcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtatacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg
      361 tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa
      421 aaaactccag ctataccgga agatatttat tattaacagg agaggtttgc ttttctgtgt
      481 aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact
      541 gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat
      601 tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata atataactta
      661 gctgtgatat gtgtaagaat gtttaggcaa tatattatta tcgctaatgt tgttttggtt
      721 gggagagatt ctccttg
//
LOCUS       DAZFDP010000531          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFDP010000531 DAZFDP010000000
VERSION     DAZFDP010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..732
                     /locus_tag="ACHJ1G_005799"
                     /pseudo
     CDS             5..732
                     /locus_tag="ACHJ1G_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          193 a          203 c          197 g          139 t
ORIGIN      
        1 tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt
       61 tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag cccgtgaact
      121 cagcctgtat gaatcacagc tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc
      181 ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc agctggcaga
      241 acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      301 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc aatgtgccgc
      361 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aaggataagc
      421 acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt tacccggtca
      481 aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg ttaccccttt
      541 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg
      601 aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag
      661 caggattttt acgccagtgg cccgaaccag aagtgggcag gagacatcac gtacttacgt
      721 acagatgaag gc
//
LOCUS       DAZFDP010000532          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFDP010000532 DAZFDP010000000
VERSION     DAZFDP010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>731)
                     /gene="nleL"
                     /locus_tag="ACHJ1G_005800"
     CDS             complement(<1..>731)
                     /gene="nleL"
                     /locus_tag="ACHJ1G_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4767386.1"
                     /translation="QENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKI
                     SLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYM
                     ENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYP
                     VTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTN
                     WMSFFLYKDGEVQNTFGYSLSNLFS"
BASE COUNT          253 a          153 c          100 g          225 t
ORIGIN      
        1 agaaaaaaga ttgctcaatg aatacccaaa tgtattttga acttctccat ccttatataa
       61 aaaaaaggac atccagttag ttgatgtatt aggagataac atatcaacca tatgactcaa
      121 agaacacatc attgccatac catcatcctg tgatgataac aaaacccagt tataagccaa
      181 atcggaatca tcatcccagt caggcttccc agagccatcg cacaatccaa aatcactctg
      241 ctgtgtgaca ggataaactt tttcattttt taaatatact tcatagagtt ccctaaatcg
      301 ttcctgcatt ccatcacgcc gtgaaccatt cattacaatt tgtataaagg aactattata
      361 agataccatc gcacctggat gtcgctcaaa atacatccct gcctgcaata aactaccatt
      421 ctccatatac atcattattg aattattaaa ccaagacatt atagtatcaa aaaagtcatc
      481 acatataggc tcgagccatt gctgtatatt tgcgttgttc aaataaggat gaatactaaa
      541 tgaatcagcc aaagacatct taacataggg ccggaacgcc gctaattcgt caagagatat
      601 ttttttgata agttcttcag cggcccttac tttatcatgg ttatacttat catcagatgt
      661 attcatagtc aataaaattg atttatttga ttttgtatag ttatccaaca cagataatat
      721 attttcctgt a
//
LOCUS       DAZFDP010000533          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFDP010000533 DAZFDP010000000
VERSION     DAZFDP010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>731)
                     /locus_tag="ACHJ1G_005801"
     CDS             complement(<1..>731)
                     /locus_tag="ACHJ1G_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4767387.1"
                     /translation="VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNG
                     QLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQ
                     QMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMN
                     DVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQI
                     KGKLSANQIEGDIVKTVGKAFPRDSR"
BASE COUNT          155 a          208 c          160 g          208 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa c
//
LOCUS       DAZFDP010000534          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFDP010000534 DAZFDP010000000
VERSION     DAZFDP010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..583)
                     /gene="sctD"
                     /locus_tag="ACHJ1G_005802"
     CDS             complement(<1..583)
                     /gene="sctD"
                     /locus_tag="ACHJ1G_005802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFO4767388.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFN"
BASE COUNT          227 a          140 c          155 g          207 t
ORIGIN      
        1 aattaaaaaa tggagttaaa tcagtggttg attcgcaacg cccataaagc gcgacatttt
       61 tcccatgcca taccagagtg attgcatgaa gtttgttttc ttttagctgc tggtatacat
      121 cagctctggt aaacgctggg ggttcagctt ttggggaaag caaaacgtaa ctgccgatgg
      181 cgccagacaa cgcaatcgca gcaccaatca cgctagccag gataagacgc tttttacgtc
      241 gatttttggc agagaacctt tctggcacat cagacaatgg caaggtgtgg tcaatatcac
      301 caatgatgaa gcaacatccc gctatatcga tgacctttcc gacaggtaat gactgatctg
      361 cttcataggg ggtgggctgt ccatcaatcc aaaattcaac cggagataaa aggaaaacac
      421 cttcctcacg tatatcaagt aaaaactgat tgaggccttg ttcaagggga aaataaacaa
      481 tatcgttatc ttctgtgcct atcgttaggt ttcccatcgg aagttgtaat tcccgattac
      541 gcatcgcacc attgagaagt tttattttat atgaggataa catttttaaa ttttattcat
      601 cctggtggtt gaacaatgag aaaaaatatg gtgaacttac atcaactaag aatagagtag
      661 aaaggaagct gttgtacagt actatttaat ggaatattca taattaatga ttacgtgagt
      721 ttccaatgg
//
LOCUS       DAZFDP010000535          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFDP010000535 DAZFDP010000000
VERSION     DAZFDP010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..345
                     /locus_tag="ACHJ1G_005803"
     CDS             <1..345
                     /locus_tag="ACHJ1G_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4767389.1"
                     /translation="ITKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKDTPDAVE
                     KMQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVVLYDQMN
                     RGGPAGNAVVMH"
     gene            368..>725
                     /locus_tag="ACHJ1G_005804"
     CDS             368..>725
                     /locus_tag="ACHJ1G_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767390.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPL"
BASE COUNT          184 a          158 c          222 g          161 t
ORIGIN      
        1 attaccaaag cgtatttcga gctgggaatg acgttcccgg aactgtatga cgacagccat
       61 ccggaagccc tggcccgtaa tacccagaaa attttccgtt ggctggataa agacacccct
      121 gatgctgttg aaaaaatgca ggctctgtta ccggcgatcg aaaaggcgat gccgcctttg
      181 ctggtggccc gtatgcgcag ccacagttct gaatattacc gtgagatcgt cgaacggagg
      241 gatcggctgg tgaaggatgt cgatgatttt gttgcgtcag cggttgtttt gtatgaccag
      301 atgaatcgcg gcggcccggc agggaatgct gtggtgatgc actaaaagca cggtgttcgg
      361 gggttttatg agcagcaagc ttcatggtct tgtctgggaa gggtgcgcct tcaccggcat
      421 gatcttatcc agggtggcgg ttatggcccg tcttgcagac tacagcaatg acgagggcgt
      481 gtcatggcct gccattgaaa ctatccggcg tcagatcggt gcaagaagtg aatccacagt
      541 gaaatcggct attgcagaac tggcgaaaga gggctggctg acgaaggaag agcgtaaggt
      601 cggtgggcgt aatgtaagca atatctatcg gcttaatgtg gaaaaactcg aagcagctgc
      661 ggcggcggcg cgtgagtcat ataaaccgaa aagaaaaatt agcccggcaa aaaatgaccc
      721 gttaa
//
LOCUS       DAZFDP010000536          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFDP010000536 DAZFDP010000000
VERSION     DAZFDP010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..710
                     /locus_tag="ACHJ1G_005805"
     CDS             <1..710
                     /locus_tag="ACHJ1G_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO4767391.1"
                     /translation="SQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYE
                     SGRITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAAR
                     KARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIA
                     ENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWP
                     ANRVRDLLPWKVDLSSQ"
BASE COUNT          184 a          184 c          213 g          138 t
ORIGIN      
        1 gttcacagat gcccccggcg gtctggttcg cgtacagtcc ggaccggaaa ggtatccatc
       61 cacaaaatca cctggccggt tacagcggtg tgcttcaggc cgatgcttac ggtggttacc
      121 gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggctcat gcccggagaa
      181 aaatccacga tgtgcatgca agagcgccca cctacatcac cacggaagcc ctgcagcgta
      241 tcggtgaact gtatgccatc gaggcagagg tccggggctg ttcagcagaa cagcgtctgg
      301 cggcaagaaa agccagagcc gcgccactga tgcagtcact gtatgactgg atacagcaac
      361 agatgaaaac actgtcgcgt cactcagata cggcaaaagc gttcgcatac ctgctgaaac
      421 agtgggatgc actgaacgtg tactgcagta atggctgggt ggaaatcgac aacaacatcg
      481 cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggatgttc gcgggttccg
      541 acagcggtgg tgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca
      601 atgtggagcc agaaaagtgg ctgcgttacg tcattgaaca tatccaggac tggccggcaa
      661 accgggtacg cgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaata
//
LOCUS       DAZFDP010000537          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFDP010000537 DAZFDP010000000
VERSION     DAZFDP010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>717)
                     /locus_tag="ACHJ1G_005806"
     CDS             complement(<1..>717)
                     /locus_tag="ACHJ1G_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4767392.1"
                     /translation="AGYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYAL
                     LVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTRDMILNELHKHVFL
                     KDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSRINKNVVVLAGNHE
                     INFNGNYTARLANHKLSAGDTYNLIKTLDVCHYDSERKVLTSHHGIIRDEENACYCLG
                     ALQVPFKQMKNPTDPEELVN"
BASE COUNT          223 a          143 c          110 g          241 t
ORIGIN      
        1 atatttacta attcctcagg atcagtagga tttttcatct gtttaaatgg aacctgcaaa
       61 gcaccgaggc aataacatgc attttcttca tctcttatta ttccgtgatg actggttaaa
      121 actttccttt ctgagtcata atgacaaaca tcgagcgttt taattaagtt atacgtatca
      181 ccggcagaga gtttatgatt tgctagtcgt gcagtatagt taccattaaa attgatttca
      241 tgatttccag ccagaacgac gacattttta ttaatacggc tatctttatt actctccata
      301 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
      361 tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt taaaaaaaca
      421 tgtttgtgca actcattcaa tatcatgtct ctcgtattta ataactcgta gagcacttta
      481 ttatttttat gcttgtatgc agatgaagcc atgttctcgt gatttaataa ttcacataat
      541 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatgc ataaagtaca
      601 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
      661 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gccagca
//
LOCUS       DAZFDP010000538          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFDP010000538 DAZFDP010000000
VERSION     DAZFDP010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..656)
                     /locus_tag="ACHJ1G_005807"
     CDS             complement(<1..656)
                     /locus_tag="ACHJ1G_005807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4767393.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTD
                     "
BASE COUNT          234 a          151 c          118 g          214 t
ORIGIN      
        1 ttatcggtac caaataaaat cttatcacta ccacagattt ttatcgactg gtaaaccata
       61 tccgccctaa cccacgcagt atcgccatag agattaggaa gttttgcaat caattcgatc
      121 gcttcctgat tatctgtcgc gagccccata tgacacataa caaaattcac atttggatat
      181 ttaagtgcca tctcataaac caactgtggg ctggattcat aatcattaga agtatggcta
      241 actaccggga gatcatattt ttgagcaagg cgtatatatt cctgaacttt atctgaattg
      301 aaagccattt ttgaatgata tgggtgaact ttgatgccat aaatgatatc tcgattgctg
      361 gcaatcatat cttcaaaatc ttgatcacaa ctttccagac gaggcttaat ccacataaac
      421 gcgccaattc gctttgaatg ttgacgaaca agtctgagca tacgttcatt attatcatgc
      481 tgtgtaactt gatcttcatc cggaatcggg ttctggtcat ggtcaacttc tactgcagat
      541 ccacaagaac ataatgcaaa atcaatatta tacttgtcca gagatgccag aaaaacatct
      601 tctggcatac ggattttcac aaagtcacca atatgagtgt gtgcatcaat tatcataaca
      661 aacatcctaa ttgaaattta aaaatcataa agatcagcag caggtaaacc ttcaatt
//
LOCUS       DAZFDP010000539          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFDP010000539 DAZFDP010000000
VERSION     DAZFDP010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..91
                     /locus_tag="ACHJ1G_005808"
     CDS             <1..91
                     /locus_tag="ACHJ1G_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4767394.1"
                     /translation="HLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
     gene            93..362
                     /locus_tag="ACHJ1G_005809"
     CDS             93..362
                     /locus_tag="ACHJ1G_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4767395.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            477..>712
                     /locus_tag="ACHJ1G_005810"
     CDS             477..>712
                     /locus_tag="ACHJ1G_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO4767396.1"
                     /translation="MPLIPDINSSSFSPELEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERML"
BASE COUNT          188 a          124 c          211 g          189 t
ORIGIN      
        1 gcatttgcag gtcaggtttg gtgacgggcc gtggcaggat gttaaggggc tgaatgaagt
       61 gggcagtgat acaggcagaa caggagaatg acatgaatat actaaaaaaa cttatgcagc
      121 gtctgtgtgg ttgcggaaag catgatggcc gtgaacacgt gcagtcgctt acagcacaac
      181 tgcgactggg accggcagac attctggagt cagatgagaa tggcattatt ccggagcagg
      241 acagggtaat cacacaggtg gtgatactgg atgcggataa aaagcagata cagtgcgtgg
      301 taagaccgct gcaaattctg cgtgctgacg ggacgtggga aaatattggc gggatgaagt
      361 aacccgacag cttcacaaaa ccggagtccg gctccggttt ttgtgttgcc atataaggca
      421 gatgtttgtt acagctattt aagtctggag ttcaaataaa aatagggagt tttgttatgc
      481 cattaatccc ggatattaat tcatcttcgt tcagtcctga gttggaggtt tttcgtgccc
      541 gaattgcagc caatgggcgc ggagatatta cagtaggtgg tgaaactgtc agtattgtgt
      601 atgatgctac taatgggcgc ttttcatcca gtggcggtaa tgacggattg ctttctgagt
      661 tattgatttt gggatttaat aatggtcctc gagcccttag tgagagaatg ct
//
LOCUS       DAZFDP010000540          711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFDP010000540 DAZFDP010000000
VERSION     DAZFDP010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>711
                     /locus_tag="ACHJ1G_005811"
     CDS             <1..>711
                     /locus_tag="ACHJ1G_005811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4767397.1"
                     /translation="YIELIDAKNLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERS
                     VRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTIFDILQNKSDIQTLKTECY
                     CLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKV
                     SHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKEC
                     VDMYVPNPLKNNIKLYYK"
BASE COUNT          262 a          134 c          119 g          196 t
ORIGIN      
        1 tatacattga gttgatcgac gctaaaaacc ttttcctcat cgccagtaat catctccctg
       61 ttgatgaact acataataca tcacaattta ttatagataa tatccttcaa acggtacata
      121 aaccagaacg atctgtaaga ttagctaagc aggaccaagg atataagaat cattatttat
      181 cagatgaaat gttagccgga aagaaagaac tttacgactt cacccctgaa agcatttaca
      241 gggccatgac tatatttgat atattacaaa ataaaagtga tatacagacg ctaaaaacag
      301 aatgttattg ccttctagcg gaatgccata tgtctttggc acttcatgga aaaagtgaac
      361 ttgaacttgc tgctcaaaaa gcattagagc ttttagatta tgtatcagac ataaccactg
      421 tcgatggaaa aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag
      481 tatctcatat cttatttgaa caggctaaga tacacccaac tgatatagcc tctctctact
      541 actatagggc actagtccac tttcataatg aaaaaattga agaggcaagg atttgtatag
      601 acaaatcact acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag
      661 atatgtatgt gcctaacccg ctcaaaaaca acattaaact ctactataaa g
//
LOCUS       DAZFDP010000541          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFDP010000541 DAZFDP010000000
VERSION     DAZFDP010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..704
                     /locus_tag="ACHJ1G_005812"
     CDS             <1..704
                     /locus_tag="ACHJ1G_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO4767398.1"
                     /translation="LYHCDHRGLPLALISKEGTTEWCAEYDEWGNQLNEENPHQLQQL
                     IRLPGQQYDEESGLYYNRHRYYDPLRGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLG
                     LEVFPRPFPLPIPWPKSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDI
                     TMIASRGNVADTGITDRVNDIINDRFWSDGKKTDRCDVLQELIDCGDISAKDAKSTQK
                     AWNCRHSRQSNDKKR"
BASE COUNT          223 a          156 c          174 g          156 t
ORIGIN      
        1 acctgtacca ctgcgaccat cgcggcctgc cgctggccct tatcagcaag gaagggacaa
       61 cagaatggtg cgcagaatac gatgaatggg gtaaccagct gaatgaagag aacccgcacc
      121 agctgcagca gctcatccgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt
      181 ataaccgcca ccgctattat gacccgctgc gggggaggta tatcactcag gatccgattg
      241 ggctgaaggg gggatggaat ttttatcagt atccgttgaa tccagttacg aatacagatc
      301 ctctggggtt agaagttttt cctagaccat tccccttgcc aattccatgg cccaaaagcc
      361 ctgcacagca gcaagcagat gataatgctg caaaagcatt gacaaaatgg tggaacgata
      421 cagcatcaca aagaatattt gactctctaa tattgaataa tccgggacta gcattagata
      481 taacaatgat agcttctcgt ggaaatgttg cagacacagg gataactgat cgtgtcaatg
      541 acataataaa tgacagattc tggagtgatg ggaaaaaaac cgacagatgt gacgtacttc
      601 aggaactaat tgattgtggt gatattagtg ctaaagatgc aaaaagcaca cagaaagcct
      661 ggaattgtcg tcactccaga cagtcaaacg ataaaaaaag atagccctt
//
LOCUS       DAZFDP010000542          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFDP010000542 DAZFDP010000000
VERSION     DAZFDP010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>708
                     /locus_tag="ACHJ1G_005813"
     CDS             <1..>708
                     /locus_tag="ACHJ1G_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767399.1"
                     /translation="GDLLSATLFHAPTIFYQYSKLIDEVKYCISNKKITGSVIARFTL
                     CLTSTLLTMSPLLMLNGAVKTGSIVRTIGRGVSYVDIPLALAILGDSWYKAYKHGSSD
                     NPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSLEKGTPPQMMSLFIVNILN
                     LLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVDLQHTKNITMPLFKTKDRI
                     SYAFNGQRTSPEDQKQI"
BASE COUNT          253 a          139 c          115 g          201 t
ORIGIN      
        1 ttggtgattt actatcagca actttatttc atgcaccaac aatattctat caatattcta
       61 aactaataga tgaagttaag tattgtataa gtaataaaaa aataacaggt tcggttatag
      121 cacgatttac tctatgttta acaagtacat tactaaccat gtcaccactg ttaatgctta
      181 atggagcagt taaaacaggt agcatagtaa gaactatagg taggggagtg agttatgttg
      241 atataccatt ggccttagct atattaggtg actcgtggta taaagcttat aaacatggtt
      301 cttctgataa cccaaattct gctcagagat ttatatcgca agaagcggcc tttaaaacca
      361 cccagcgggt attaacacaa ggattaagtc tgatgtcctc tttatcggga gcaatcatgc
      421 gctctcttga gaaaggcaca ccaccacaaa tgatgtcttt attcatcgta aacatactaa
      481 atctattatt tcatcaaaat ccatatgaag gggcatcagc aagtgctaat gctttgaaaa
      541 gatcaactta ctcccataat ccggacatac taaatactca ggcaatagct ctttgtgttg
      601 acctccagca tacaaaaaat ataaccatgc cacttttcaa aacaaaagac aggatatctt
      661 acgcattcaa tggacaaaga acatccccag aagaccaaaa acaaatac
//
LOCUS       DAZFDP010000543          697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFDP010000543 DAZFDP010000000
VERSION     DAZFDP010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(232..>697)
                     /locus_tag="ACHJ1G_005814"
     CDS             complement(232..>697)
                     /locus_tag="ACHJ1G_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO4767400.1"
                     /translation="LNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSH
                     VLKDMPLRKQAEILFCLGGGFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQV
                     FGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          240 a          157 c           96 g          204 t
ORIGIN      
        1 aagcactacg atatatgcag tatcctattc atatattacc accatatcag acaaatcgaa
       61 tcacaacgag ttctatcgta ctctttcatc ttcgacactg taaattttaa gaacattcgg
      121 ctcatgccac taaaccaaga cataataaca acggagcaat aaatgttccc actgaacatg
      181 tgcaaaccta aaaagacagt gttacaataa tattcatcca tggtctctaa atcaacgcca
      241 cgcaacagga taatacaatg aaaatatttc aggaaaagat tcttttgcat gctccgttag
      301 catatcagtc agcacagcgg tacaagtaaa aacattattt cttccttgta gcctgtctaa
      361 aatatcattg taataataaa ctgagccaaa tacctgagga tctgttttat aagcttgttc
      421 aatcaatcca tttgcatatc tccgtagaat ctcaggagaa tcatactctg taccaaacat
      481 atcactcgat gagtatttac agaaaccccc ccctaaacaa aataatattt cagcctgttt
      541 tcttagtggc atatctttca atacatggct ccccataatc atatctacat gctgagcgtt
      601 cagttcccaa ccatcaagaa agggattcca gacacaggca agtttccttt gctgatcatc
      661 agcaatcatt ttataatctg atttattgga attaagt
//
LOCUS       DAZFDP010000544          690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFDP010000544 DAZFDP010000000
VERSION     DAZFDP010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHJ1G_005815"
     CDS             <1..198
                     /locus_tag="ACHJ1G_005815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767401.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>690
                     /locus_tag="ACHJ1G_005816"
     CDS             234..>690
                     /locus_tag="ACHJ1G_005816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767402.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEID"
BASE COUNT          205 a          123 c          150 g          212 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc
//
LOCUS       DAZFDP010000545          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFDP010000545 DAZFDP010000000
VERSION     DAZFDP010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>689
                     /gene="sepL"
                     /locus_tag="ACHJ1G_005817"
     CDS             <1..>689
                     /gene="sepL"
                     /locus_tag="ACHJ1G_005817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4767403.1"
                     /translation="ESQQLTQKNSSNSYSPLLNLQNELAMITSSSLSETIEGLSLGYR
                     KGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKELSLKNSGLLEQHDPNLAMFGNMPK
                     GEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWLGVGELNQEGIQKI
                     KKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKT
                     SSIISDLCRVI"
BASE COUNT          227 a           99 c          157 g          206 t
ORIGIN      
        1 tggaatctca acaattaacg caaaaaaatt cttctaatag ttattctcca ttattaaatt
       61 tacaaaatga attagcgatg attactagtt catcgctttc tgaaacgatt gaagggctaa
      121 gcctgggata tcgcaaaggc agtgcaagga aagaagagga aggtacgaca acggagaagc
      181 tgcttaatga tatgcaagag ctgcttactc tgaccgatag cgataaatta aaagaactat
      241 cattaaagaa cagtgggctg ctggaacagc atgatcctaa tttggctatg tttggtaata
      301 tgccaaaagg ggagattgtt gcacttatat cttctttatt acaatctaag tttgttaaga
      361 ttgaattaaa aaagaaatac gccaaattat tattagattt attaggcgaa gatgattggg
      421 agttggctct gctttcctgg ttaggggtgg gtgagttaaa tcaggaaggt atccagaaga
      481 tcaaaaagct ttatgaaaag gccaaggatg aggattctga aaagggagcc tctttacttg
      541 actggtttat ggagattaag gatcttccag agcgtgagaa gcacttaaag gttattatta
      601 gggcgctatc gttcgatctg tcttatatgt cttcttttga agataaggtg aaaacatctt
      661 caattattag cgatttatgc cgggtaatc
//
LOCUS       DAZFDP010000546          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFDP010000546 DAZFDP010000000
VERSION     DAZFDP010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>686
                     /gene="ehxB"
                     /locus_tag="ACHJ1G_005818"
                     /gene_synonym="hlyB"
     CDS             <1..>686
                     /gene="ehxB"
                     /locus_tag="ACHJ1G_005818"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO4767404.1"
                     /translation="YVIWSLFISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMA
                     VSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEI
                     SIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPE
                     IQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGKSTLTKLLQRFYIP
                     ETGQILIDGH"
BASE COUNT          204 a          109 c          163 g          210 t
ORIGIN      
        1 tatgttatat ggtcattgtt tatatcaccc ttattacgtc gacgtcttga tgataagttt
       61 ctcaggaatg cagaaaatca agcttttctt gtcgaaacgg taacagcaat aaatacaatt
      121 aaatccatgg cagtatcacc acagatgatt gctacatggg ataaacaact ggccgggtat
      181 gttgcttcca gtttcagggt aaatctggtt gcaatgacag ggcagcaggg gatacagctg
      241 atacagaaaa gtgtaatggt aattagtcta tggatgggag cacatcttgt catatcggga
      301 gagataagta tcgggcagtt aatcgcgttt aatatgcttg caggtcaggt cattgctcct
      361 gttatcagac tggctcatct ttggcaggat tttcagcagg ttggtatttc tgttgagcgc
      421 cttggtgatg tattaaatac accggtagag aaaaagtcag gcagaaatat actgccggaa
      481 attcaggggg atatcgaatt taaaaatgtc agatttcggt attcttctga cggtaatgtt
      541 attttgaata atattaattt atacatatca aagggggatg ttatcggtat agttggccgt
      601 tctggttcag gaaaaagtac attaacaaaa ctgcttcagc gcttttatat accagagacc
      661 ggacagattt taattgatgg gcatga
//
LOCUS       DAZFDP010000547          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFDP010000547 DAZFDP010000000
VERSION     DAZFDP010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..580)
                     /gene="fimE"
                     /locus_tag="ACHJ1G_005819"
     CDS             complement(<1..580)
                     /gene="fimE"
                     /locus_tag="ACHJ1G_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4767405.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKL"
BASE COUNT          205 a          171 c          139 g          171 t
ORIGIN      
        1 ttaatttttc gtttatgaga ttatttcttt cccataatcc ggcaaaacga gcagcattac
       61 tggcggtata acgtacagta tggcgaatat ttcgatgccc gagataatcc tgaattaaac
      121 gagtatctgc accacgctcc gccagttcat aaccgcaagc atgccttaac atatgaggat
      181 gagtctgcgt tacggttcca gcttcaatac cggcatcgcg aataatgcga taggcctgct
      241 ggcgagaaag ccgactccca cggcgagaaa taaatatagc gtcggtccgg tcagcgcctt
      301 tccagttagc acgttcctgg gtccagcgtt ccacggcttc acgctcatca aaacgtaacg
      361 ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt tattcgacct tcattaaggt
      421 caagatcctg ataatgcaga tcaagcagtt cactaatacg catcccatgc cgatatgcca
      481 acagaataag acaataatct ctggctcccg ttgccccgta acaaaccgcc tgcatcatgg
      541 cctgaacttc tttaccggta agataacgac gtttactcac aatagtagta ctcctgactg
      601 agatatattt gaatgaatac ctataggaaa cctcaatcgg tcaaaattag cccaatagat
      661 aaaaagataa ccacacacga agtgat
//
LOCUS       DAZFDP010000548          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFDP010000548 DAZFDP010000000
VERSION     DAZFDP010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(66..425)
                     /gene="traA"
                     /locus_tag="ACHJ1G_005820"
     CDS             complement(66..425)
                     /gene="traA"
                     /locus_tag="ACHJ1G_005820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO4767406.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(458..673)
                     /gene="traY"
                     /locus_tag="ACHJ1G_005821"
     CDS             complement(458..673)
                     /gene="traY"
                     /locus_tag="ACHJ1G_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO4767407.1"
                     /translation="MIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDH
                     LNRFPDFYNLDAIKEGAEETDSIIKDL"
BASE COUNT          201 a          174 c          124 g          181 t
ORIGIN      
        1 cagtgtttcc gggaaacgat atttcttaag tttattctcg tctcccgaca tcgttttatt
       61 tcctgtcaga ggccaacgac gctcataccc acagcaataa atacagagat gatggcaaag
      121 ccgaccagga acttgacgtt tttggtcatc atgtacatga ccgcaccgac cagcacctca
      181 gccagaacaa cccatttaac gatactggag tccttaccga aggtcgcctt aaccgtatcg
      241 ttacctttag ccatcaggtc gcctttacca gcagccatag ctaactcagg gaacaacatc
      301 atcagaacag caaccgggat cactgcacga accaggcgaa gcatattcag acgagtgaat
      361 ttagaaaaaa acgatttctt ttcaacgggc gcagaagcac cctgaacact taaaacagta
      421 ttcataagtt acctctattg tggatagatt aataaaccta aagatcttta attatcgaat
      481 cagtttcctc tgctccctcc tttattgcat ccagattata aaagtcagga aaacggttca
      541 gatgatcacg taacctgact agcacctcat tagtttttgt tctcccacta cgttcccggg
      601 cacctaagag cttgtgatta gtagcatcgt ccaataccag taaaacttta ttgcccggag
      661 caggccttat tatatttctg
//
LOCUS       DAZFDP010000549          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFDP010000549 DAZFDP010000000
VERSION     DAZFDP010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..>678
                     /locus_tag="ACHJ1G_005822"
     CDS             161..>678
                     /locus_tag="ACHJ1G_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFO4767408.1"
                     /translation="MSVLLLPLSLQAADIRRSGEDTFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLR"
BASE COUNT          153 a          156 c          178 g          191 t
ORIGIN      
        1 tcgcaaaaca tggcgggagg tatcgtcagg aggctggtga ttgttggtat gacatgaata
       61 aaaggttttt cgtttgactg gaaaattatg ttgcaggaac gagagggctg aggaacaaag
      121 cttattccgg cgctgtccgt ccttgctgtc actgacactg atgtctgttt tattattgcc
      181 tttatctctt caggccgcgg atatccggcg tagtggagag gatacattta tcattcagca
      241 gcagcgtcag gaagcccttg agcaacaact gacgccttca gcccctgatg ttcgcctttc
      301 tgcacctggc tcttttgccc ataagattaa ttttcctgtt gaaacgccct gttttcagat
      361 taaacagacg gaactgaagg gggctgatgc gttaccacac tggctgcctt tacaaaaaat
      421 cgccaacggg gcggtcgggc attgcctggg ggcgaaagga attaatctgc tgatgagtac
      481 attgcagaac cgtctggtcg atcatggtta tgtcaccacc cgtgttctgg caccttcgca
      541 ggatttaaaa agcggtatcc tccggctggt tattattccc ggtgttgtgc gacatgtgcg
      601 tctgacaccg gacagtgatg actatattca gttgtattcc tcattcccgg cacacgaagg
      661 ttctctgctg gatttacg
//
LOCUS       DAZFDP010000550          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFDP010000550 DAZFDP010000000
VERSION     DAZFDP010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            578..646
                     /locus_tag="ACHJ1G_005823"
                     /pseudo
     CDS             578..646
                     /locus_tag="ACHJ1G_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          206 a          155 c          165 g          150 t
ORIGIN      
        1 agagaattta tcattttgct attgccatta atttatcatt ccgataaagt tcacccatca
       61 gcaggacgca ctactcacca gggcggtgaa tatacaacga ttcgaatatg aatctacggc
      121 gctgacaaag cgcaataacc aaagtgaact ttggggtgaa tgcagaagct aaccttctcg
      181 gcggaggcgc tttgcaatga ttacgcgacc ggagttagtc gcccggctgt attcaccgcc
      241 aaagttcatc aggaggttcc atatgacacg cagaactcag ttcaaaggca attctcgttc
      301 tcgtcgtcgt gagcgtttaa aggcaaaggc attagctaac ggcgtactgg cccgcgaaga
      361 agcaataagt tcagaagtat tacaccgccc aactctaagt agagcgcaga ttcaggctaa
      421 aggtactcac gaaacgcctg atcgcataga agacgctaag ccaattaagt tcatggcaca
      481 ggacgtgatc tggcagcagg aagaatacag acgcaatctg gagcgagcgg ccattgtgta
      541 cgcgaatgag tttggacata agcaaccaga aactggtgta tgtcttccaa atgtagctct
      601 ttacgcggca ggctaccgga aatccaaaca actgacagca agatgacttg tgttggtcgc
      661 cagaaaatga aattag
//
LOCUS       DAZFDP010000551          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFDP010000551 DAZFDP010000000
VERSION     DAZFDP010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>667)
                     /locus_tag="ACHJ1G_005824"
     CDS             complement(<1..>667)
                     /locus_tag="ACHJ1G_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4767409.1"
                     /translation="TYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFST
                     NEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLR
                     DPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHY
                     VGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAG
                     IFNY"
BASE COUNT          213 a          124 c          102 g          228 t
ORIGIN      
        1 aataattaaa aatgcctgcc atataatgct tttgtttaaa tttatgtttt gcacaatacc
       61 gatactgatt gctatttaca ggtttaagta aatcttcttt actccacgga gaagctgctt
      121 tggcgatcaa aaaactacgt gacaccggat agtcagccca ctcatgccag ggttttgtag
      181 gtcccacata gtgtataaac acggtgtcat cattgactgg attatcaact ttgtctttta
      241 attcatagtt aatactatat cgggtattat atttctccga aataaacttc actttaccat
      301 tcaataatac attcagtaca tcttgatcaa ggtgggtgat tttacttacc caatctgggt
      361 cacgcaacat ttcaatagct tttgacgaaa tgttatttag attccactca tcaatattta
      421 tcagtaaaaa accagcattg aaatatccag aagctaactg tggtgtagtt aataccgagg
      481 ctcgattttg ccaccattca acatctcttt cagctacaac tgcggcaatt tcattagtag
      541 aaaattgata atctaagagt tctttaatac tacccttgca agcaatatct gcatcaagat
      601 atagtatttt ttcatgttta tgatagaaat aatctgcaat tataaatcga aaatatgttg
      661 cgtaagt
//
LOCUS       DAZFDP010000552          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFDP010000552 DAZFDP010000000
VERSION     DAZFDP010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          207 a          123 c          123 g          210 t
ORIGIN      
        1 gtttccctac cttatatacg tttagctcgc ctacggcttg atttaagcgg tagaaactta
       61 tttaaccttc aactactaaa accattcttt cttttttgtt tgttgttggt tgttgtctgt
      121 gtctatgtgc taacatcgct aacgcgtagc acacaaggtt attgcactcg tcagagtatt
      181 gcacaataga ttttctctta aatagattga tgatcgttag attggcgtaa gcctcatcaa
      241 tggaactatt gaagcgcaca gcgcatagac actaacaacc attcgcttac gctcaggtta
      301 ttgcacaagt aaatttcaat cgttagatgg tagttagtta attatcgttc tgcgcacagc
      361 gcaagactat tagccatacg caagccgtag gctatttgca catagtagat tacatattcg
      421 ttgtgagcgg aaagcgaaca tagatcagct aactactaac aaaaaacaaa aaacaaaaac
      481 tatctgttat gacaagcatc agtctaactt gtgcaaggtt ctggcgaatg ccaatagatt
      541 aagggatagt cgaagactcc ctaacgggta tccctataat tattctttaa aagataaaag
      601 aatttataaa agatagttta attgtagttt aattatagta ttgtaggaac tcatttcctg
      661 ggt
//
LOCUS       DAZFDP010000553          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFDP010000553 DAZFDP010000000
VERSION     DAZFDP010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..472)
                     /locus_tag="ACHJ1G_005825"
     CDS             complement(<1..472)
                     /locus_tag="ACHJ1G_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4767410.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSDTS"
     gene            complement(540..>662)
                     /locus_tag="ACHJ1G_005826"
     CDS             complement(540..>662)
                     /locus_tag="ACHJ1G_005826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767411.1"
                     /translation="HMTLEFSVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          156 a          190 c          160 g          156 t
ORIGIN      
        1 gagaggtgtc gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc gttttcccct
       61 tgttgtcagt tacgcgaaga taatccccgg agaaagtcga cacacggctg taagccacac
      121 ccgccatcgc atacgcgctg aaccattcat tcacgcgcac agacggcccc gccattacgc
      181 tgaaccagcg gttacgcacg gaatcttcat gccagcgggt atcgctgtaa cgggtaagct
      241 ggcgattctt gtctcctgca tagctgaatg acgtcaccag ccccagcgtg tccgtgaatt
      301 cataacggta tttcacgtta atcccgttaa gattatcgct accgggagcg ttcgtccggg
      361 catgaagata ccccgcgctc agcgtggcct gctgctcaga cgcccatgca ggcgcaccgg
      421 atacggtcag acaaatggct gcggacaaaa tggcggcata aagtttacgc ataattacct
      481 ctcgcttttc tgcaataaaa aaaggcgtca tttctgacgc ccgttctggg ttataaaatt
      541 cagctgatac tgatacctgc tgtggatttt ttcatcacca caaccagcag atcgctgata
      601 cttgctgtgg gataccagtt atttaccagc catgctgaca ccgaaaactc cagcgtcatg
      661 tg
//
LOCUS       DAZFDP010000554          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFDP010000554 DAZFDP010000000
VERSION     DAZFDP010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..>662
                     /gene="nleG"
                     /locus_tag="ACHJ1G_005827"
     CDS             83..>662
                     /gene="nleG"
                     /locus_tag="ACHJ1G_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO4767412.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDE"
BASE COUNT          199 a          142 c          134 g          187 t
ORIGIN      
        1 tgtttgaatc ctatcacata tttagtatct tgactcctgt caccaatatg gttcaataac
       61 atagactaaa gagggagaaa atatgcctgt catattaaac ttttcgagtg aaagagtatt
      121 gtctgaaagt gaactggagg ctttacgtca tgtcggaaga gttagtcaga gtgaacagct
      181 tgttgtgaga ggccgcacta tgagacttca tcatatttca tttatggaca gttttagcgt
      241 ggaaccggtt tccggcggac tgttggatcg ccttagtgca agagggcatc gccttctcgc
      301 agaaaatctg gagatacagc ttaacagagg ccatactttt ttgcaggcat tccgtttata
      361 tatggaacag agccgtgcaa ccccatgtac gcgccaaaac gtcagtagtg caatacaaaa
      421 caaaattaac tctcatgcat ttactgttag tcatcaagac ttttcctgtc atgaacaaca
      481 cctgaattgc ccgataacat tatgcattcc tgaaactggt gtttttgtca gaaatgcaaa
      541 aaactcagaa atatgttcat tatatgacca caatgcactc actgaactta tccgacgcaa
      601 cgctccacac cctctcagcc gtgaaccctt tgttccggaa atgattgtca gcaaagatga
      661 at
//
LOCUS       DAZFDP010000555          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFDP010000555 DAZFDP010000000
VERSION     DAZFDP010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(280..>661)
                     /locus_tag="ACHJ1G_005828"
     CDS             complement(280..>661)
                     /locus_tag="ACHJ1G_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4767413.1"
                     /translation="SWAVNHYRDNAIAYKEQRDKATSIIADMQKRQRDVAELDARYTK
                     ELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSMGDGESPGLTSDAELNYYRL
                     RGGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          149 a          165 c          162 g          185 t
ORIGIN      
        1 cttctgctgc ggtaactaac tggcgaaagt cgaagtcatg attcgcaatt aattcattca
       61 tggcgttgcc tgcttctttg aaatgaacct ttgccgcaca ggaaaccagc ccaccgaggc
      121 tcgccagcac taactggtat cctcaaaggc ccattccaaa gggtcaggtt cggtgtttat
      181 tgtgcgctgc ggtgcgcggt gaaataccgg tacaaaaatg ccccgcatct gcgaggcatt
      241 ttcctgaaag tcacttgtta aatttcagtg aaattaaaat tattttaagc actgcgtcct
      301 gatgtattcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac ctcggagacg
      361 gtaataattg agttcagcat ctgatgtaag tcctgggctt tctccatcgc ccatgctgct
      421 ggctccggtc gttgactttg cacaggtggc ggagacttgc aggcgcttac gcccagcaga
      481 aacatcagca cggagacttt cgatagtcgc attagcatca gcaagctcct ttgtgtatct
      541 ggcgtcaagt tctgctacat cacgttgccg cttctgcata tcagcgatga tggatgtggc
      601 tttatcgcgc tgctctttgt aggcgatggc gttatcacgg taatgattaa cagcccatga
      661 c
//
LOCUS       DAZFDP010000556          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFDP010000556 DAZFDP010000000
VERSION     DAZFDP010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(103..>659)
                     /locus_tag="ACHJ1G_005829"
     CDS             complement(103..>659)
                     /locus_tag="ACHJ1G_005829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767414.1"
                     /translation="HEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACD
                     LVPTQGNDPHHVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFE
                     GDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYA
                     ERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          232 a          127 c          103 g          197 t
ORIGIN      
        1 catgaagctt agaagactta aacaaaaata aacaacatca ttgtgatgat aaatataaaa
       61 taggcatggc gaaaaaaaat caccacgcct aaaatataat aattatggta gcatcattga
      121 tacataatcc acaccaatcc ttgagctata ctgagacgct atagcctgat atctttctgc
      181 ataaccagtt ctcagttgag atttaagttt gagtcggaca ggaacatttt gcacgttgcc
      241 atccatatta cttaaaaaca cggcagaaat aatatttttt tcttcgccat caactgttgt
      301 tccatgattc aacaccacca tataatcaac aacaggaagc gttttatccc cttcgaaaat
      361 agagagatat tttctttgat ttttatgcat tacatatata tatttcgaat gttcagcaaa
      421 tggcaatgct ttactctgac tggcgttaaa aagctccaga actttaatga gcctgtgcgg
      481 acttaatctt acatggtgag ggtcgttacc ctgagtagga accaaatcac atgccgcaga
      541 tacacataaa taccatttgt tcgactctgt atcaaagaaa atagtgccag tagaaatatg
      601 accatcttca aaattctttg aagacaaatt catatttaaa gcatgataca tttcgtgat
//
LOCUS       DAZFDP010000557          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFDP010000557 DAZFDP010000000
VERSION     DAZFDP010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          208 a          157 c          127 g          166 t
ORIGIN      
        1 aaagtagaag aacttcatct ctttagcaaa cgccattaac aattgccttt ccatttagtt
       61 aatcgttttg ttttctttaa gattagtcct attagacaaa tagcaaaatg gaataactac
      121 agcactaaga cgttccgcta tatagctatt acctgttccc ctgacataag gagcagatat
      181 acctcaaccc cctattcccc agtgctgacc gggagcggcg gcggtataaa aaggaacaag
      241 gaacaaatgg cagaacaagc aaccatgttt gtcccccccg cataaccgta ccattcgcat
      301 taagaaatat tcccatatct atcccgcaaa agaggacccg tcatacatca agtccgtttt
      361 actgggcatc agattatcgc cgtaattagc tcagtgaaag ccgggtaaac ggttagcaag
      421 ctcagtggct caaaagtgca tagcgctaag atgggcctgg ttacacctgc cctttgctgc
      481 agatcgtgtt gatcggtgtg atgccgccac acgtttaagt caatctggaa aactcacaat
      541 gcgctcttct ataaagagga ctctttgata aagtaacccg aaaagggaaa gctaactgat
      601 atcaatgtac caccttagta atcatccctt atatacagac aaaaagaagt tgcctgaa
//
LOCUS       DAZFDP010000558          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFDP010000558 DAZFDP010000000
VERSION     DAZFDP010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>658
                     /locus_tag="ACHJ1G_005830"
     CDS             <1..>658
                     /locus_tag="ACHJ1G_005830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO4767415.1"
                     /translation="SNKIGQRVYTVVSEMSIDAWITKEPKPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTLITFSNEEI
                     "
BASE COUNT          197 a          119 c          146 g          196 t
ORIGIN      
        1 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgatgcat
       61 ggataacaaa agaaccgaag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacgaaatca
      601 taagaattct cgaatatatt gataatacgt taattacctt tagcaatgaa gaaataat
//
LOCUS       DAZFDP010000559          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFDP010000559 DAZFDP010000000
VERSION     DAZFDP010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..627
                     /locus_tag="ACHJ1G_005831"
     CDS             <1..627
                     /locus_tag="ACHJ1G_005831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4767416.1"
                     /translation="TQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGNIFAAVD
                     EKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKERRPTGM
                     FAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKFNLTEMS
                     FVEMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          208 a          137 c          134 g          178 t
ORIGIN      
        1 acccagaata gccctgcgca gctacttaaa atagccagaa gtggcgacat catcggccga
       61 gccccgctgc cttttatctc tgatgccgaa accattgagc acatacaagg gaacattttt
      121 gctgcagtag acgaaaagac ttctgaacta ttctttttta cagttacaaa agatatgcat
      181 atctccttgc gaaataaaat tcagttagaa aaattcaata aaaaaaatag agggtttgaa
      241 ggactggcat ggaaagcaga aggccggatg ctgtttgtcg ctaaagaacg acgccctaca
      301 ggcatgtttg cctatcagct ttctccagat ctgctgagtg caaaacaagt aacgattcca
      361 gaggaactaa atgatataca tgttaaagat atatccggcc tggactttaa taatgaaagt
      421 ctgatgattc tgtccgatga atcgcgtaag ttactcaagt ttaacctgac agaaatgtct
      481 ttcgttgaga tgatggacct gacaaaagga aatcattctc tgaccagtga tttgctccaa
      541 cctgaaggca ttgtaactct gtctgatgaa agcatttatg tcgctagtga acctgatatc
      601 ctcgctaaat ttgtccctaa taaataagtc tgtcactagc cttagtattg agcgacc
//
LOCUS       DAZFDP010000560          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFDP010000560 DAZFDP010000000
VERSION     DAZFDP010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..496
                     /locus_tag="ACHJ1G_005832"
     CDS             <1..496
                     /locus_tag="ACHJ1G_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4767417.1"
                     /translation="EVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDFYISYL
                     RRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSK
                     LAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIR
                     ATTE"
     gene            468..>653
                     /locus_tag="ACHJ1G_005833"
     CDS             468..>653
                     /locus_tag="ACHJ1G_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767418.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          191 a          141 c          162 g          159 t
ORIGIN      
        1 tgaagttgcc aggcatttaa gtctgaccga tcgccagctt cgcagattgc agaaattgcc
       61 gggtgccccg atatcaaata agcgagggca actggatctg gatgcctggc gcgattttta
      121 catatcgtat ctgaggagaa gtaaaaacga tgtgcctgat ggcgatagcg aagacgacta
      181 tgaggaaaaa ttgcttattg ccagatggga actgacagca gaacaggctg ttacacagca
      241 gttaaaaaat gaggtgtcaa aaggaaaact tattgacacc gggttctgta tttttgccct
      301 cagtaagctg gcaatggcgt tatccagtac gcttgattcc atccctttat ccatgcaacg
      361 acagtttcct gatttaacac cgcgccatct tgaccatctg aaaaccctta ttgcgaaggg
      421 ggcaaatcag tgtgcgcggg cgggggataa attaccggat ttactcgatg aatatatcag
      481 agcaacaact gaataatatg atgagcgctg tcacaactgc attacagccc ctgataaggg
      541 cattgccggt gacgccagtt gaatgggctg atcaaaatta ttatctgcct aaagaatctt
      601 catatggtga gggcgaatgg aaaacgctgc cattccagat cgccatcatg aac
//
LOCUS       DAZFDP010000561          644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFDP010000561 DAZFDP010000000
VERSION     DAZFDP010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..463
                     /locus_tag="ACHJ1G_005834"
     CDS             5..463
                     /locus_tag="ACHJ1G_005834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767419.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            563..644
                     /locus_tag="ACHJ1G_005835"
                     /pseudo
     CDS             563..644
                     /locus_tag="ACHJ1G_005835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          208 a          116 c          110 g          210 t
ORIGIN      
        1 ctatatgaaa atatactgga caagaaaacg tattcctgaa ctttcagcac ttccaccttc
       61 attaagaaag aaaaatttta ctgacgcata taatgcagca tcttctcata tcgaatactg
      121 gataggtgct ggcgtctcat ttatctcgat gatgatttta tccagagtat atgactttct
      181 tttacccgcc caagatactt tcccgggtga tatcatcaga agtttatgtg ttgtgtgtcc
      241 atccatatta atctggtttc agttttcggt atatgtcatg aggaaatatt acagacatat
      301 tctggtgcga ggaaaagaaa ccgaaaccat ttcagaaagg ttgatacgtg aggctgatac
      361 acgagaatat gaactatggc gtccggtccg tcgctttttc tccattgttt tcctcattgt
      421 tctattaggt tgcattcatt cactgataac aactataaaa taaaagttat ggctgttctg
      481 atacaggaaa ataaacgttc attctatggt tacctcttcc tgttatatag aagaatttta
      541 aacgacaagt aagggaatat aaatgaaaat aacaaaaaat aaaaatgcaa ctataaaaaa
      601 cagtcatcag tcggctgctt ttcaatgttt accttatcat tttc
//
LOCUS       DAZFDP010000562          644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFDP010000562 DAZFDP010000000
VERSION     DAZFDP010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..198)
                     /locus_tag="ACHJ1G_005836"
     CDS             complement(52..198)
                     /locus_tag="ACHJ1G_005836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767420.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          205 a          127 c          121 g          191 t
ORIGIN      
        1 gtctgtcaaa atagtagatt agatcgaact tatattacag ctatttagtt tctaaaatgg
       61 aatttttaac gttttatact tcacattaca atctgtacca taagcctcct cagcgaggtt
      121 gcctgaatac cctgtcgtgc ccatttttcc atcggacgca gttgtctgct ctctcgaact
      181 gctggcaata aaaatcaacc agacagcttg atgtccgtta catgcaggac taatcacgat
      241 tggttaccgt ttacaaaact tgcgtaacat tggaggagaa cgaataatat tctctagctg
      301 gtacgcgttc catagcgaaa cgggcaaggt ccaacagcaa gagctgaaac tcagggggat
      361 ttatcagtcg atttattcgc catggatgaa tcacgctgta cggttatggt agctcttagc
      421 gacaaggcat aatcatcagt acaccaatgt aacaaccgtt gaaaaagact cattatttta
      481 tgaaccagcc atcccatttc cagagtggta aatatgattt gttaaagtgt aacagtactc
      541 tgcgtagcct ttttagattc atagtttaat gtttctaaat acattaaatt gcactataag
      601 agaaagacat aatgtgagga taaaaatgcc attttcaata aaaa
//
LOCUS       DAZFDP010000563          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFDP010000563 DAZFDP010000000
VERSION     DAZFDP010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>643
                     /locus_tag="ACHJ1G_005837"
     CDS             <1..>643
                     /locus_tag="ACHJ1G_005837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4767421.1"
                     /translation="SEDDVRPEALRRFELMVEEAVRHAEEAKKNAGEAETSARNAGIS
                     ASKAEASAANADTSAEDASESARQAAESAASAKKSEEASSSSASEAAQKASESLQSAT
                     DAELSKKTAESAAGNASRDATTATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPP
                     GPKGDPGPAGPQGPKGDKGERGDTGPAGATGERGPAGDAGPAGPQGPKGDRGER"
BASE COUNT          178 a          162 c          231 g           72 t
ORIGIN      
        1 tgtcggagga tgacgtccgt ccggaggcac tgcgtcgttt tgaactgatg gtggaagaag
       61 cagtgcgtca cgcagaggag gcgaagaaga atgccggaga agcagagacg tccgcgagga
      121 atgccggcat atcagccagt aaggcggaag cgagcgccgc aaatgctgat acttcagcag
      181 aggatgcatc ggagtcagcc cggcaggcgg cagaaagtgc agcctctgca aagaagtcag
      241 aggaagcgtc ctcgtcctca gcctctgagg ccgctcaaaa agccagtgag tcattacaaa
      301 gtgcaacaga tgccgagttg tcaaaaaaga cggcagaaag tgcagccggt aatgcatcca
      361 gagacgcaac gacagcaaca gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg
      421 aacaaagcag gatagcggcg gaagaagccg taaaccgaat ccccaccgtg gtggggccac
      481 ccgggccaaa gggagatccg gggcccgcgg gtcctcaggg gccgaaggga gataaaggag
      541 agcgtggcga caccggcccg gcaggggcaa ctggtgaacg ggggccggca ggtgatgctg
      601 gtccggcagg cccgcagggg ccgaaaggcg acagggggga acg
//
LOCUS       DAZFDP010000564          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFDP010000564 DAZFDP010000000
VERSION     DAZFDP010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>636
                     /locus_tag="ACHJ1G_005838"
     CDS             <1..>636
                     /locus_tag="ACHJ1G_005838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4767422.1"
                     /translation="KRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAK
                     LELVRMNIIKQQGGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKL
                     GDCYPSKQGDTKDTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAE
                     DLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWA"
BASE COUNT          211 a          144 c          153 g          128 t
ORIGIN      
        1 aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca
       61 atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc
      121 aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt
      181 ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggagg ttcccctaaa
      241 atgagggaca tccctcaaaa cgagggaaaa tcccctaaaa cgagggataa aacatccctc
      301 aaattagggg attgctatcc ctcaaaacag ggggacacaa aagacactat tacaaaagaa
      361 aaaagaaaag attattcgtc cgagaattct ggcgaatcct ctgaccagcc agaaaacgat
      421 ctttctgtgg ttaaaccgga tgctgcaatt cagagcggca gcaagtgggg aacagcagaa
      481 gacctgaccg ccgcagagtg gatgtttgac atggtgaaga ccatcgcacc atcagccaga
      541 aaaccgaatt ttgcagggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt
      601 aaccaccgcg atatgtgtgt gcttttccgc tgggcc
//
LOCUS       DAZFDP010000565          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFDP010000565 DAZFDP010000000
VERSION     DAZFDP010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>622
                     /gene="yibB"
                     /locus_tag="ACHJ1G_005839"
     CDS             <1..>622
                     /gene="yibB"
                     /locus_tag="ACHJ1G_005839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO4767423.1"
                     /translation="TAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMII
                     FTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNP
                     EYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDE
                     SKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFY"
BASE COUNT          201 a           99 c          130 g          192 t
ORIGIN      
        1 cactgcatat tttgacattg gtagagggga ttggactgct aataaagggt tccgtgaaaa
       61 actagctcgt tcagttgatg tttattttag ttactttgag cgtctggccg cacttgaaaa
      121 tgagatgatt attttcactt ctcccgattt gaaaccaagg gttgaagcga ttcgcaacgg
      181 aaaaccaaca acggttatcg ttatcgatat aaaaaagaaa tttagatata tcagaagtcg
      241 tatcgaaaaa attcaaaaag atgagtcgtt tacaaataga cttgagcctc gacaattaaa
      301 aaacccagag tactggtcac cagagtatgt tttggtatgt aaccttaagg cctattttgt
      361 aaataaagct atcaacatgg gcttagtgaa aacgcctctt gttgcgtgga tagattttgg
      421 ctattgccgt aagccaaatg taactcgagg attgaaaatt tgggatttcc catttgatga
      481 aagtaagatg catcttttca ctataaaaaa agggcttact gtcacatctc aacaacaggt
      541 ttttgatttc atgattggca atcatgtata tattattggg ggcgcaattg ttggttcaca
      601 gcataaatgg aaagaattct at
//
LOCUS       DAZFDP010000566          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFDP010000566 DAZFDP010000000
VERSION     DAZFDP010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>620)
                     /locus_tag="ACHJ1G_005840"
     CDS             complement(<1..>620)
                     /locus_tag="ACHJ1G_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767424.1"
                     /translation="MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNW
                     RYCITDAYTGNTRNYLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVM
                     RDGMMAAGVITSGEWLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQD
                     AEWLASRRFTIEDIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMT"
BASE COUNT          128 a          178 c          170 g          144 t
ORIGIN      
        1 gtcatagtga gaaatgcgcg gcttgcctcg ctgaaattgc tgtaggtgct gttgctgtat
       61 tcctgcaaaa aaatcggcga gacgttgaac attcgggcga tgtcttcaat ggtgaagcga
      121 cgggaggcca gccattcagc atcctgatta ctcatgccca gctgctgata gctcataccc
      181 ccttcaagga tgggcgtttt tccggcgttt ctggcccctt tgtagcgttc cagtgcggct
      241 aatgcctgtt tgcctttcac gccgtccagc cattcgcctg acgtgataac ccctgccgcc
      301 atcatgccat cacgcatcac gctcgcgccg tggcgttgtt gggccagccc aagccccagc
      361 gattcgcggc agatggttac aggtgagcgc cccataaaac cgtcatccgt ggagtaacga
      421 aggtggagaa cctcccacgg taaatagttg cgggtgtttc cggtgtaggc gtcagtgatg
      481 cagtagcgcc agttgtgttc gcctgtctgc tccacgttca ccgactgcgg cgggtaagga
      541 tgtaaagcca ccggaaaacc atcacgcccc cactgaatca ccgcataagc attaccgttt
      601 aacaggcagt ggcggatcat
//
LOCUS       DAZFDP010000567          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFDP010000567 DAZFDP010000000
VERSION     DAZFDP010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..366
                     /locus_tag="ACHJ1G_005841"
     CDS             67..366
                     /locus_tag="ACHJ1G_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4767425.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            363..>616
                     /locus_tag="ACHJ1G_005842"
     CDS             363..>616
                     /locus_tag="ACHJ1G_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4767426.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNI"
BASE COUNT          184 a          142 c          164 g          126 t
ORIGIN      
        1 ggaactgcaa gaaatagatc tattgcacga tatatataaa gaacagcaca ccggagaata
       61 aaaataatga aacttaaata ttctggctta actgccagtg gcaacactca ccctaaattt
      121 acgcgcggcg atatttaccg cgaccagtac ggcggcacgg taatgattaa gggcgtggcg
      181 ggacggtgcg taacctaccg ccgtgaaggt tacgaatatg attgcgtgat gcctgtttat
      241 cagttccggc gtgatttttc tctggtacag accgcgccgc ataacgtgcc caccagcaac
      301 gccagggcac gggcaaacat ccagaagctg aaaaccatga ttaacggatt caggggcaag
      361 aaatgaaaag cgcaccgaac ttaaaaaaac agccttacga caagatgacc gaagtcatta
      421 tttttgcggg tagtgatgcc tgggcacatg cgaaacagtg gcaggaacag gacgggcgac
      481 tggctggcga taatgtgcct cccgttgtgc tggctgatga tcaactggat gaactggcag
      541 acctgagaat catcgacgag gggcgctatt gtgtccggct gtacaaggca ggccacatca
      601 ggccatcaaa tattaa
//
LOCUS       DAZFDP010000568          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFDP010000568 DAZFDP010000000
VERSION     DAZFDP010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..170
                     /locus_tag="ACHJ1G_005843"
     CDS             <1..170
                     /locus_tag="ACHJ1G_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO4767427.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
BASE COUNT          189 a          151 c          116 g          152 t
ORIGIN      
        1 accatggtgg tgagcgggga gcgctactgt acagcctgat cgggacgtgc aaactgaatg
       61 acgtggatcc agaaagctac cttcgccatg tgcctggcgt catagcagac tggccggtca
      121 accgggtcag cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg
      181 tcaatacggc cctcgctgta cgcttacgca taaatattga atacatttac tgagcatgca
      241 aaagaaaaag acaaactaat cccggccttt cacagaattt cctctatcag aggctgagtt
      301 gacaacctgc tcacccagga aatagtaaca cccgaaaaca tgaagagact ctgataaaca
      361 atctcgatac atattcctca tctttcacag catctcactt ctttaacagg aattattatt
      421 atctacgcct cctcctttca tgtatccctc atgaatgatt aagataaaca tactgtgttt
      481 gttatccgta tgataacaaa cacataaagg agtaaacatt tatctggatg atgattacaa
      541 tgttcacgac tgtcagagac acacctctga tacattagaa aaaccaatgt ttcccttaaa
      601 aatggagc
//
LOCUS       DAZFDP010000569          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFDP010000569 DAZFDP010000000
VERSION     DAZFDP010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..429
                     /locus_tag="ACHJ1G_005844"
     CDS             <1..429
                     /locus_tag="ACHJ1G_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4767428.1"
                     /translation="AMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQ
                     KAAQWLQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYED
                     GEMWSAMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            429..>607
                     /locus_tag="ACHJ1G_005845"
     CDS             429..>607
                     /locus_tag="ACHJ1G_005845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO4767429.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFY"
BASE COUNT          156 a          125 c          176 g          150 t
ORIGIN      
        1 gccatgcttc atggtgccga acctgtaagc caaacttaca agtcacaaca tacgcagttt
       61 gaacaagttg ctgacctcta cgaaatgcaa tttgatgacg gtcgcacttg tgcctttcac
      121 actgatgcgc aaaaggctgc gcaatggctt caggcatgcg acggaaacag ggttcaggaa
      181 tacgtgaagc tggagcgatt gcgtaatgcg ctatcgggca actctccggt aactccggat
      241 ggttggatat gctgtagtga gcgaatgccg gataagttaa ttccggtaat ggtcatgtat
      301 gaagacggtg agatgtggtc tgcaatgtgg aatggcaatc gctgggatga tggcaccgaa
      361 tatccggatc cgcactcagt tacgcactgg cgtgaaatgc cagcagcacc gcagcaggag
      421 gtgaagtgat ggactccttc gcgaaatata cgattattga ctggatagca ttccttcagg
      481 ttttgctcat ctggttttat atggcttaca ggagtgggca gtggattgtc agtgtagcct
      541 gtagcaaggg atggcgttgg tggaaccgaa agaataaaaa agcgctggcc ttggattcgt
      601 tttacga
//
LOCUS       DAZFDP010000570          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFDP010000570 DAZFDP010000000
VERSION     DAZFDP010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>599
                     /gene="espX1"
                     /locus_tag="ACHJ1G_005846"
     CDS             <1..>599
                     /gene="espX1"
                     /locus_tag="ACHJ1G_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4767430.1"
                     /translation="HKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQ
                     EIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFP
                     ELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYSQLCKKLSIFKSIPSRLIKKSAYCS
                     TENTITNKFDVVFSYCGNNVKEFILLLPYNKSLEMHELN"
BASE COUNT          242 a          106 c           83 g          168 t
ORIGIN      
        1 cacaaaggta tcacattact tagattggat gtttttgatg acagaacaag cgaatgtctt
       61 tctgaagaga ttaagatata ccaagaatgc catgaaaaat tcgttaattt tttaaaggca
      121 aacttcaacc aagaaatata cccacagcta tatactcctg aaatatttta tgaggcctgc
      181 agaaatctcc agtcatttta tgatcatcag gaaacgtcac aaaacgcaaa atacagtgca
      241 atagacaaga aaaaaagtca tcttaaccag gaaatcaaaa agcttattaa aaaaaatatt
      301 ttccctgagc tatacaatga gcaatgcaat aaaatcccct cctcatcgat agatgataac
      361 cagggaataa tgtggcagaa ctttaaaact agcaatattg catattcaca actatgtaaa
      421 aaattatcta tatttaaaag catacccagt cgattaataa aaaaatcagc ttactgctca
      481 actgaaaaca caatcaccaa taaatttgat gttgtctttt cttactgcgg aaataatgta
      541 aaggaattta tacttttatt accctataat aaatctctgg agatgcatga acttaacga
//
LOCUS       DAZFDP010000571          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFDP010000571 DAZFDP010000000
VERSION     DAZFDP010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..220
                     /locus_tag="ACHJ1G_005847"
     CDS             <1..220
                     /locus_tag="ACHJ1G_005847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
                     /protein_id="HFO4767431.1"
                     /translation="AQEHLLLQCSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQI
                     LLPGEIHIKESGKREYKGRDSGLFMQPL"
     gene            complement(228..581)
                     /gene="cmi"
                     /locus_tag="ACHJ1G_005848"
     CDS             complement(228..581)
                     /gene="cmi"
                     /locus_tag="ACHJ1G_005848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO4767432.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
BASE COUNT          172 a          131 c          104 g          183 t
ORIGIN      
        1 agcacaggaa catcttcttt tacaatgttc atatgatgat aaatacgatt ttaacgccag
       61 cactcaccgt ggcatcatcg gagagtcgct cacaaggctc ggggcgatgt tttctggtaa
      121 agagtaccag atactgcttc ctggtgaaat tcacattaaa gaaagtggta agcgagaata
      181 taagggccgg gattccggcc tttttatgca gcctttataa cagtgtatta gtcattcgca
      241 tattcaacat atccctcctc acaatggtat atgccataat acattctctt accctccggg
      301 ccttcagctt taaatgggac aagaagtgtt ttttcatctt caacatctgt taaacttctc
      361 tcaatctttt cccatgtaat tttcgggatt tctgtaccaa gaagttgaac atcgttgtcc
      421 cagcgtttca ttctttgcag gtaatcattt tttacaaacg ttctggcctg ttcatcgctg
      481 acctgatagc aggccggacc tttatcttca gaccagaaaa aaacagcagc aagagcaata
      541 atcgttaaaa taaaaataaa tttcatgcta attactttca taaaatctcc
//
LOCUS       DAZFDP010000572          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFDP010000572 DAZFDP010000000
VERSION     DAZFDP010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..314)
                     /locus_tag="ACHJ1G_005849"
     CDS             complement(<1..314)
                     /locus_tag="ACHJ1G_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4767433.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSS"
     gene            complement(362..571)
                     /locus_tag="ACHJ1G_005850"
     CDS             complement(362..571)
                     /locus_tag="ACHJ1G_005850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4767434.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
BASE COUNT          117 a          200 c          141 g          126 t
ORIGIN      
        1 gatgactgcg acgcctgttt cagtcgggca ttcacggaac tccattcatc ggccagagaa
       61 atcaggtgtc cggtggcaaa ggcacctgca aatgcccccg ccgttccggc agctgaagcg
      121 cggatttccg tcaactggct gttcagctca gccagggcgc gtcgctgctc ccgggcgact
      181 gcggcagcct gacgcccgcc attctgcagg gtccggtaat attcactgcc catgcgggaa
      241 gcccgctgga tctccgactg gaatgactgc gaatttgccg aaattttgat aatcagttca
      301 cgtaacgtcg ccattcacct ttctccgggc gtaaaaaaac cgcctcagcg gttctcatca
      361 ttcatgactg tgctgcgaag ctcagcgcgt cttccagcgc cgcaaacgga tccacctccg
      421 gcttatcctc atcctcgccc cagcagagca tggcgtcctt cagtgcaaca ttcatcccct
      481 gtgccccaaa aaccgctttc acgatctgtg cattacggat atccccgcgc tcatcaccca
      541 gcggggatac cctgtcgaac tccatccaca tcatcgcctc gctc
//
LOCUS       DAZFDP010000573          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFDP010000573 DAZFDP010000000
VERSION     DAZFDP010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>568)
                     /locus_tag="ACHJ1G_005851"
     CDS             complement(<1..>568)
                     /locus_tag="ACHJ1G_005851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO4767435.1"
                     /translation="MPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSG
                     FLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQ
                     RDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKS
                     FDPTSKEEIKILTDTSKLIYDLFGVKSEK"
BASE COUNT          172 a          116 c           92 g          188 t
ORIGIN      
        1 tttctccgat ttaaccccga acaaatcata aattaattta gaagtgtctg taagtatttt
       61 aatctcttcc tttgaggttg ggtcaaacga cttcgcgaag ctaattaatt gtggtgcagc
      121 atctcgcatt ttgcttaaaa tatcaggatc gagcgttcct tcattcacca gatgtgacat
      181 cttatccaga tagccatcaa aattcatttc tcccccatca gccagacgct tcatctctcc
      241 caggtacttc ttcatatcac gttgagataa tttttcaaac tgagctttca ggtagctctc
      301 attattttca ttaatataca ctgtttctga taaatctgcg taggcactag aatatgaaag
      361 tgaaattaac agcgaagcaa ataattttaa gctgttgtca tcatgttcct ggcaggcatt
      421 aacaaacgtt aaaaaaccag aacctattgc ttgaaaatgt atattcgcta aaggttcatt
      481 atccttagat tcctcataaa atagctgaac cgtggaggga aggtcattct tattttctac
      541 atcaaaagta cattgttcaa tgggcatt
//
LOCUS       DAZFDP010000574          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFDP010000574 DAZFDP010000000
VERSION     DAZFDP010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..>566
                     /locus_tag="ACHJ1G_005852"
     CDS             243..>566
                     /locus_tag="ACHJ1G_005852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767436.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGSDKLANFNFFLIKKSNLS
                     GLYMYH"
BASE COUNT          197 a          107 c           93 g          169 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtgac aaacttgcta atttcaactt tttcttaatt aaaaaatcta
      541 atctttctgg tctctatatg tatcac
//
LOCUS       DAZFDP010000575          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFDP010000575 DAZFDP010000000
VERSION     DAZFDP010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..376
                     /locus_tag="ACHJ1G_005853"
     CDS             <1..376
                     /locus_tag="ACHJ1G_005853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4767437.1"
                     /translation="KMPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDSISNGYTYFDS
                     VHMDCEKISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKTVETHRLNIMK
                     KLDVHSGIELIKTALRMGVCTI"
     gene            complement(395..565)
                     /locus_tag="ACHJ1G_005854"
                     /pseudo
     CDS             complement(395..565)
                     /locus_tag="ACHJ1G_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          191 a           96 c          106 g          172 t
ORIGIN      
        1 aaagatgccg tatattgatt caataaaatt gcttgaagca ggtgcgaaag gatgcgtatg
       61 gaaaaccagt cacccggcga aactaaatcg tgctattgat tcgattagta atggttacac
      121 ttattttgat agtgtacata tggattgtga aaaaatatct tccaggtatt cctctgataa
      181 tcaacttaca aatcgtgagt cagaaatatt acaactcata gctgatggta aaacaaacaa
      241 agaaatcgct aatttcttgc agttaagcag aaaaacagtc gaaactcata gactcaatat
      301 catgaagaaa ttagatgttc acagtggtat cgagttgatc aaaacagctt tacgtatggg
      361 tgtttgcact atataaaaaa aagaggcttt aggctcattt tgctgaagcc ttacgattgt
      421 ctagttcatt aagcctggta tatacaatgt atattttggg gcatattgct gtctcttttt
      481 ttggttatcc attgcaaaac ccgcattata agtccccagg cattgccacg ttactccaca
      541 acgtgaaaaa tggttatata aaatc
//
LOCUS       DAZFDP010000576          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFDP010000576 DAZFDP010000000
VERSION     DAZFDP010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>558
                     /locus_tag="ACHJ1G_005855"
     CDS             <1..>558
                     /locus_tag="ACHJ1G_005855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO4767438.1"
                     /translation="RMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGK
                     RFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQ
                     DLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEY
                     KKLFALPDESELQQKDAEELFDWLI"
BASE COUNT          159 a          110 c          135 g          154 t
ORIGIN      
        1 cagaatgacg ttatccctca tggtgcaacc cggtcttttt gaccgatata tggagcgaaa
       61 aggttctgtt gccagggaca gtggttttct tgcccgttgt cttatcagta aacctgcaac
      121 cacgcagggg aaacgcttta ttaacggagc tgtgattccg ggaggatcat taacggcatt
      181 tcatgaacga ttaatggaac tggccagagg cagtattgaa aaatccagtg aggatgaacg
      241 gtattgtctg catttttcgc ctgaagcgca aaaaatattt attgagcact ataatgttct
      301 ggagcaggat ttatcccctt ccggaccctt aagcccgttt cggggacatg tttcaaaaaa
      361 aacagaaaac attgcaagaa ttgcagcgtt atttcagtat ttcagctatg gtgaagggaa
      421 aatctctgcc gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata
      481 taaaaaatta tttgcgttac ctgatgaatc tgaactgcag cagaaggacg ccgaggagct
      541 gttcgactgg ctcattga
//
LOCUS       DAZFDP010000577          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFDP010000577 DAZFDP010000000
VERSION     DAZFDP010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>553)
                     /locus_tag="ACHJ1G_005856"
     CDS             complement(<1..>553)
                     /locus_tag="ACHJ1G_005856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO4767439.1"
                     /translation="GSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDL
                     VRISGPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTA
                     WPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRI
                     QHGEPLVESRYLYDPLGRRMAKRV"
BASE COUNT           69 a          172 c          182 g          130 t
ORIGIN      
        1 acccgtttcg ccattcgccg tcccagcggg tcgtagaggt agcggctctc gaccagtggc
       61 tcgccatgct gtatccgcgt gtggaacacc aggcggtgct ggctgtcgta gtggtagtgg
      121 tgggtccgct cgtcgtccgt ccgtatcaca cccgtcggga tgcggtccgt cttctccgtc
      181 agtctgccgt attcatcata gtggtaaaca tagtgcgcat cctctgcgat gcggttatcc
      241 ggccacgccg tgagcgtgct gtccgggtgt agctccgggt ccggcaggcg gtttcccgcc
      301 gggtcggtgg catacgggat gcggatgtcc aggcccggtg cgagggtgcg cacgctctcc
      361 agcctgcccg tggcactgta gccgtattcc cgcgtctgtc gcgggccgct gatgcgcacc
      421 aggtcgccgt tgtcattcca cccgtagtca cggtcataca ccaggctgtt caggtgctgg
      481 ctctgtaact ggcctgcggg ggtgtatgtg ctggtcagtt catatgcggc attactgcct
      541 gccctgctgc cga
//
LOCUS       DAZFDP010000578          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFDP010000578 DAZFDP010000000
VERSION     DAZFDP010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..310)
                     /locus_tag="ACHJ1G_005857"
     CDS             complement(<1..310)
                     /locus_tag="ACHJ1G_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4767440.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     K"
BASE COUNT          161 a          148 c           82 g          162 t
ORIGIN      
        1 atttttttga ccgcgcgttt ccggacgtat tctgttctcc tgtcccttta tatcgtcgga
       61 atacccgccg ctcttcaaat cccattccca actcagaatg tagtctgttg accgcttgtt
      121 ttatttcggt caggttcacc ggtgaaaccg gagtccggcg cgccttacgc aaacactctg
      181 ctcgtttctg tgccgccact tttcttttct ggtcatcact tagctgtacc atcacttttg
      241 cccatcgttc agctgctctc cggtacagtc cttttttctc cagacattct gccacgtgat
      301 catgtagcat aagtgacctc cgattatcta cagactgcca tcctgaattt accttccctt
      361 aatgaaataa caataaaaaa caaaccacgc aaaaacaata aaacaacaca caaaaaaaac
      421 taaataataa acaaaaataa tcaccttatt ttattatttt ttgagggggc aattactgaa
      481 caaaaaacgc tgactatata ctcaaaacca aacaactatt ctgccaatca ggtatcatgg
      541 caacacacgg aat
//
LOCUS       DAZFDP010000579          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFDP010000579 DAZFDP010000000
VERSION     DAZFDP010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>553
                     /locus_tag="ACHJ1G_005858"
     CDS             <1..>553
                     /locus_tag="ACHJ1G_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4767441.1"
                     /translation="FGFILALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLN
                     GIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLY
                     EASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYR
                     FAGHTDIFITWIYKLTLNFQQYHI"
BASE COUNT          154 a           98 c          111 g          190 t
ORIGIN      
        1 tttggcttta ttctggcttt agcactggct aaaaaaaata tacgtttcgc aaaagtctgg
       61 cgattgatat ttatactgcc atatgccata cccacatttg taacgttact tattttccgt
      121 cttcttttaa atggaattgg tccggttaac tcgtttctga acagtatcgg aatgaattca
      181 gttgattttc tgtcggttcc attaaatgca aaaataacag taattgccgt ttgtgtttgg
      241 gtaggggcac cgtattttat gctattgatc gcgggtgcat taacaaacat atcccgagat
      301 ttgtatgagg ctagtgaggt cgatggcgca actaaatggc aacagtttaa ggaaattaca
      361 ttgccaatgg tgttacatca ggttgcgcca actctggtaa tgacatttgc atataatttc
      421 aataattttg gtgcaattta tcttctgact gaaggtggtc cgactaatcc ggaatacaga
      481 ttcgctgggc ataccgatat ttttattacc tggatttata aactcacatt gaatttccag
      541 caatatcata tag
//
LOCUS       DAZFDP010000580          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFDP010000580 DAZFDP010000000
VERSION     DAZFDP010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..546
                     /locus_tag="ACHJ1G_005859"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHJ1G_005859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          179 a           84 c          141 g          142 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt agtgattata ttaatccaga gcataaaaaa acgataaagg ctttacaaga
      541 tgctgg
//
LOCUS       DAZFDP010000581          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFDP010000581 DAZFDP010000000
VERSION     DAZFDP010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..283)
                     /locus_tag="ACHJ1G_005860"
     CDS             complement(<1..283)
                     /locus_tag="ACHJ1G_005860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767442.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNH"
BASE COUNT          176 a          101 c          106 g          163 t
ORIGIN      
        1 catgattcag gtaatatgta gagatagtcc cgttaacagt gtcaccatta aaggtggtat
       61 cgaaatcacc gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat
      121 ctacagtacc cggtgcatcc atattgctcc agtcaagagc ggtagttgga ttgtcgtaat
      181 aagtccaggt gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca
      241 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
      301 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
      361 aatgattatg ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
      421 ggacatatat aacctatgga gaaaaacacg catcaaattg ataaaagggc gatatttcaa
      481 catgttgcag ataatgttat ttttgataca tacgcgttat aaatatcaaa aacacaaacc
      541 agataa
//
LOCUS       DAZFDP010000582          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFDP010000582 DAZFDP010000000
VERSION     DAZFDP010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>545)
                     /locus_tag="ACHJ1G_005861"
     CDS             complement(<1..>545)
                     /locus_tag="ACHJ1G_005861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4767443.1"
                     /translation="MLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQ
                     TSTPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGD
                     GDKKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNA
                     VWVVNSTTAAKLQKVKNANGD"
BASE COUNT          112 a          151 c          129 g          153 t
ORIGIN      
        1 taatcaccgt tcgcgttctt caccttctga agttttgccg ccgtcgtgga gttaaccacc
       61 cagaccgcgt ttttgcggta ttttttacgt aatgcaaatt tcaggtcgat cagcgggtcc
      121 gcagatgtcc acgccagaga ttcggaaggt ttaattacct gcaacgtacc aaaatcacgc
      181 tctttgtcgt tcttctctgc acggggtacg gataaaaaac cttttgcttt tttgtcaccg
      241 tcgcccacaa ccagatcgct ttcttcggtt tccgtgaagg tgtcgccaat ctcacccgtc
      301 agccatgaaa ggatgtccac atcggaaaaa tccacgattt cctgtgtggt gcgcgggtac
      361 gcatagaccg gatacagctt aatgctcacc tcgttaatct gcggggtgct ggtctgttcg
      421 cgtgccttac cctcttcacc gtggttaacg gtcgcaccgc cagcggaaac aagctgctta
      481 aactcgttgc tgctgatttt cttcacggta cagatgcggc gcatggtgga ttcatccgcc
      541 agcat
//
LOCUS       DAZFDP010000583          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFDP010000583 DAZFDP010000000
VERSION     DAZFDP010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>543
                     /locus_tag="ACHJ1G_005862"
     CDS             <1..>543
                     /locus_tag="ACHJ1G_005862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4767444.1"
                     /translation="IFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEV
                     IDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFS
                     KFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKT
                     TGTGKYDWLNPESIGVNDVTP"
BASE COUNT          182 a           96 c          117 g          148 t
ORIGIN      
        1 atttttacta agatcaatac aaggattcct cgagacacaa tgcccatcct tcgtcgttat
       61 cacctatggc ctgatggatg gaatattcgt gggctcaatg gattcatgct atcacataaa
      121 ggtagtgaag tgattgatgc tgttatcgca ggccagaatc aggcttacag ggaactaaga
      181 agaataagag ataatattca tagtgaaata tacttcaaac aaactgatga attgtcctca
      241 cttccagata cagacaaaat tggagggatt ctggtaaaaa aatacctttc aggaagtctt
      301 ttttcaaaat tcagacaaga tactattatc ccggaggcat tgagcaccct ccagatatca
      361 ggtcctgact tgattcaaag aaagatgttg caatttttca ggagtagagg ggtgttaggt
      421 gaagagttca ttaatgaaag aaaactgagt gataaagctt atattggtgt ctacaaaaca
      481 actggcacag ggaaatatga ctggttaaac cctgaatcga tcggcgttaa tgatgtcacg
      541 ccc
//
LOCUS       DAZFDP010000584          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFDP010000584 DAZFDP010000000
VERSION     DAZFDP010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>543
                     /locus_tag="ACHJ1G_005863"
     CDS             <1..>543
                     /locus_tag="ACHJ1G_005863"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO4767445.1"
                     /translation="GKKSYKERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAF
                     SFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSW
                     TLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVP
                     AKIIRFRFDSNVIDELLRIK"
BASE COUNT          174 a           78 c          116 g          175 t
ORIGIN      
        1 ggggaagaag tcgtataaag aaaggaattt atttcttttc tcaaaaggtg acaagataac
       61 aattgaagat aatgttattg ctgaagaata ttcaacaatg cctgtgaaaa atttctccag
      121 tgtcggcgct ttttcatttc cgacctgcca tttttcaggg aatatacgta taggtcgatt
      181 ttgtagtatt gcatcaaatg taaaaataat gggtggaaat catcctttga atcgttttac
      241 tactcacatg atgacttata acggtgaatt tgataagttt gctatgagtg aatttgaacg
      301 tagctggaca ttgaaaccat tcattaccaa acctgaaaat ccgattatag gaaatgatgt
      361 ctggatagga aatgatgttg tattgaaagg tggcatcgca ataggtgatg gtgctgttat
      421 tgctgccaat tccgttgtta ccaaagatgt gcccccatat gccatagttg caggagttcc
      481 tgcaaaaata atcagattta ggtttgattc aaatgttatt gatgaactgt tgagaataaa
      541 atg
//
LOCUS       DAZFDP010000585          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFDP010000585 DAZFDP010000000
VERSION     DAZFDP010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..322)
                     /locus_tag="ACHJ1G_005864"
     CDS             complement(113..322)
                     /locus_tag="ACHJ1G_005864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4767446.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(418..>527)
                     /locus_tag="ACHJ1G_005865"
     CDS             complement(418..>527)
                     /locus_tag="ACHJ1G_005865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4767447.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          118 a          174 c          120 g          115 t
ORIGIN      
        1 tccgactgga atgactgtga atttgccgaa attttgataa tcagttcacg taacgtcgcc
       61 attcaccttt ctccgggcaa aaaaacctgc cacagcaggt tttcatcatt atttatgaca
      121 ttgctgcaaa gctcagcgcg tcttccagcg ccgcaaacgg atctgactcc actttatcct
      181 catcctcgcc ccagcagagc atggcgtcct ccagtgaaac attcatcccc tgcgccccga
      241 aaaccgcttt cacgatctgc gcattacgga tatccccgcg ctcatcgccc agcggggaca
      301 ccctgtcgaa ctccatccac atcatcgcct cgctcgcact cagactgtgg cccagttcgg
      361 ataaggtgcg ccccagacgg agcgcaagtc gcatcagaaa gagaatttcc gggcgggcta
      421 cttttttctg gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc
      481 atgtaccgga ccatagacgg ccagcacctg ctcacggtcg tccggag
//
LOCUS       DAZFDP010000586          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFDP010000586 DAZFDP010000000
VERSION     DAZFDP010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..>521
                     /locus_tag="ACHJ1G_005866"
     CDS             46..>521
                     /locus_tag="ACHJ1G_005866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4767448.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFT"
BASE COUNT          162 a           87 c          106 g          166 t
ORIGIN      
        1 accaatgaaa ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt
       61 cgttataaaa gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag
      121 tcaccgcgtt tgcagtactg cgcaaactcg tgtttttgta tgtggcccga agaaagttca
      181 tattttaatc gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc gagattaagc
      241 ggatatattt ttgtcgattt ttcagtaagt tttttacgtc tattccttga aaaagactgg
      301 attgactatc ttgcaagtac tgacatgggg attgttttag tcagcgaccg caatatgcaa
      361 tcgttagcta attactggcg aaaacacaac tcagcaatat ctgctgtcat atataatgat
      421 gatggtcttg atgttgccaa tgaaaagatc agacaactat ttattggtcg ttatttatca
      481 tttaccagag gtaacacatt aactcagatg gaattcacca t
//
LOCUS       DAZFDP010000587          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFDP010000587 DAZFDP010000000
VERSION     DAZFDP010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          174 a           84 c          120 g          143 t
ORIGIN      
        1 atagataaga ttaataagat cagaattctc tttcggtgtc gcctctggct tttccaccga
       61 aaacaattac acgactacag aatgtaggaa gtgaaattgt attgaggctt agtgaaatta
      121 cacgaggaaa gcagagtgta attgagcttt agccgctctc ttcgagtaat tacacaacta
      181 cagagaataa cgagagttat taaatgtagg aagtggaatt aagaggtaga gagttacaca
      241 taagcaatca gtatgattgg ttaggtgatt actcttctgc gcacagcgca atagtatttc
      301 ttcgtgggcg ttaccgccac gatagttaca cgaagaaagt aacgctttgc tttcggtgtg
      361 agcggaacgg gccagccacg aagcccgaaa ggtagcggag taaggctgct tgccagagag
      421 tgaagctaac gccggaaggc aaaagatcat atatatatag tataaagaat attattatga
      481 tctaattaat aatctaatat agttttatta atagtcttat t
//
LOCUS       DAZFDP010000588          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFDP010000588 DAZFDP010000000
VERSION     DAZFDP010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..396
                     /locus_tag="ACHJ1G_005867"
     CDS             <1..396
                     /locus_tag="ACHJ1G_005867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO4767449.1"
                     /translation="ALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKACIAKSFLQ
                     HEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSEQFRYDN
                     NISKAMAEQLFDAVGDQNSGVLKWVKAHW"
BASE COUNT          163 a           81 c          107 g          165 t
ORIGIN      
        1 gcattatctg acactaggct taatgaggcc aaatgcttgc ttgatcatgg cttttttcat
       61 ggtgcatatt atctttgtgg gtatgcagtt gaatgtgcat tgaaggcctg tattgccaag
      121 tcatttttac aacatgagtt tccaaacaaa aaagtcgtaa atgattcata tactcatgat
      181 ttgtcgcaac ttctcaaaat tgccaactta catcaaattt tgattgctga cgcaaaaaat
      241 gatgtttcgt tggagattaa ctggtcggtc gttaaagact ggagcgagca atttagatac
      301 gacaataaca taagtaaagc tatggccgaa caattgtttg atgctgtagg tgaccaaaat
      361 tctggagttt tgaaatgggt aaaagcacac tggtaatcgg cagagagttg acaaaagata
      421 tggaattctc aggtcaattt ttgttaaaaa aactcaagtt acagaattta actattgatg
      481 ctgcaatgtg gttttattac ccagatctat cttgga
//
LOCUS       DAZFDP010000589          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFDP010000589 DAZFDP010000000
VERSION     DAZFDP010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>513)
                     /locus_tag="ACHJ1G_005868"
     CDS             complement(<1..>513)
                     /locus_tag="ACHJ1G_005868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFO4767450.1"
                     /translation="FIGYNEKFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLP
                     SSMNKIQSVAIGDKLWLVQFIPLIYGEVVNVLWLFFCKNSNVIVDYCRGLRTNITNDR
                     MLEFKNKSTEIQWKVFILYSFGFCHESIASLLSITNGSSRNAISEVYKFFGIHSKHDL
                     LMIFHTSRMH"
BASE COUNT          185 a           94 c           72 g          162 t
ORIGIN      
        1 tatgcatcct gcttgtatga aaaatcatta acaaatcatg ttttgaatgt ataccgaaaa
       61 acttatacac ttctgaaatt gcattccggg aagaaccatt agtaatagac aaaagagatg
      121 ctatcgattc atgacaaaaa ccaaatgaat aaagaataaa aactttccat tggatttccg
      181 ttgatttgtt tttaaattca agcattctgt catttgttat atttgtacgt aatcctctac
      241 aataatccac tatcacatta gaatttttgc agaaaaaaag ccataaaaca ttaaccacct
      301 ccccatatat taatggtata aattgtacca gccagagttt atcacctata gccacgctct
      361 gtattttatt catggacgaa ggcaaagaca tagcatccag ttcttctctt aagaacgatg
      421 tagctacttg tacaggaagg gagtaaaacc actctttgat atcaagacta cctataaatt
      481 ctttagagaa tttttcattg tagccgataa aat
//
LOCUS       DAZFDP010000590          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFDP010000590 DAZFDP010000000
VERSION     DAZFDP010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..441)
                     /gene="espF"
                     /locus_tag="ACHJ1G_005869"
     CDS             complement(<1..441)
                     /gene="espF"
                     /locus_tag="ACHJ1G_005869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO4767451.1"
                     /translation="MLNGISQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQAL"
BASE COUNT          136 a          112 c          135 g          116 t
ORIGIN      
        1 tagcgcctga gcaatgggcg gtaaagaccg ggatgaccca gataccaacc cgcttgtcgg
       61 cggcggcggt gccgggcggc ttggcttaag gcttgaagta tcaagacttt tcgacttttc
      121 ataggcagct aaatgatctt ttaatgcctg agcaatgggc ggtaaagggc gggatgcccc
      181 ggaagcctgt ccacttgtcg gcggcggtgg ggccggacga gagggagtaa atgaagtcgc
      241 cgggctactc acattaaaag tcgttctcgc attgatattc gacgtaccgg gagaaaaagg
      301 agattgagcc ctaatcggat tcagacgaac agcctgaggg gacactgaaa agccagataa
      361 ccccgcagag cttacccggc ttgccgcact agtaatatgc cgtcctagtg tagaaacagc
      421 ttgactaatt ccattaagca taattcatgc ctttttatat ctaatcaaca ggaataatta
      481 ttaatttgta ttaaaatta
//
LOCUS       DAZFDP010000591          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFDP010000591 DAZFDP010000000
VERSION     DAZFDP010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(213..443)
                     /gene="ynfN"
                     /locus_tag="ACHJ1G_005870"
     CDS             complement(213..443)
                     /gene="ynfN"
                     /locus_tag="ACHJ1G_005870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO4767452.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
BASE COUNT          155 a           98 c           84 g          161 t
ORIGIN      
        1 atatctgctt ctgcttttgt ttttaagttt tcaatattca tcatttcctc ttttagtctg
       61 ttatgacttt ccagttacac agtaagtcga ttatatggtg caaacgtgta aaagataaga
      121 tgaaacatcg caataatcaa catacgatag tctaaatttt acacaaacag acaaagagaa
      181 ttttcctgaa ttatcaatac aatagcatca aatcaactca agagccttat tgctgcttcc
      241 agaatttctt ctgaagtaac atgtcgatcc gcggctacat aaatgacttt atgatctccg
      301 gtcagagatg gaaaccctgc ggccattaca gtaaggtgtg ttttttcgcc atttggatat
      361 tcacgcatga tggtgttaac tccagtcatc gctggcacta ccactgctgg ttcagagtta
      421 aaaaaaacta tgattttttt catgatgtta ccgtagtatg tgagtatcca tcgaatagat
      481 accaagcaaa aaagctcc
//
LOCUS       DAZFDP010000592          488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFDP010000592 DAZFDP010000000
VERSION     DAZFDP010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>488
                     /locus_tag="ACHJ1G_005871"
     CDS             <1..>488
                     /locus_tag="ACHJ1G_005871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4767453.1"
                     /translation="IRISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLGREHRMERR
                     AVALERQLNGGVDFLSSVNNYFQSVMAEHRENKTSNKILMEKINSCLFRPDSNHFSCP
                     ESFLTCPITLDTPETGVFMRNSRGAEICSLYDKDALVQLVETGGAHPLSREPITESMI
                     MR"
BASE COUNT          152 a           78 c          119 g          139 t
ORIGIN      
        1 aataagaata tccattggta gtggccaata ttctgttcac tatgtccaac tactggatgg
       61 attttcagtt gaaccggtga gaggaggctt actggatagg ctattggggc gtgagcatcg
      121 aatggagaga agggctgtgg ctctggaaag gcaattaaat ggaggtgtcg attttttaag
      181 tagtgttaat aactattttc agagtgtcat ggcagaacac agagaaaata aaacaagtaa
      241 taaaatatta atggaaaaaa taaattcttg tttatttaga cctgattcta atcacttttc
      301 ttgcccggag tcatttttga catgcccgat aacgctggac acacctgaga ctggggtgtt
      361 catgagaaac tcacgaggtg ctgagatatg ctctctatat gataaggacg cgttagtgca
      421 acttgttgaa actggtggag ctcatcctct gagtcgagaa cctataacag aatcaatgat
      481 catgagaa
//
LOCUS       DAZFDP010000593          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFDP010000593 DAZFDP010000000
VERSION     DAZFDP010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>483)
                     /locus_tag="ACHJ1G_005872"
     CDS             complement(<1..>483)
                     /locus_tag="ACHJ1G_005872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4767454.1"
                     /translation="HRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEAD
                     EVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQ
                     RAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLV
                     R"
BASE COUNT           79 a          168 c          156 g           80 t
ORIGIN      
        1 gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac
       61 ggcagacaga cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg
      121 aaaagcggac ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg
      181 cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat
      241 ttcgccgctg aattcaccgg cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc
      301 caccagcccg gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct catccgcttc
      361 cggcacccgc tcacaccagc cgagcagcca ccacggcccc tgcggactgt ttgtcgccag
      421 ataacgatgc ggacttaagc ggagttcttc cggcagcgcc tgccagagtg cggccagccg
      481 gtg
//
LOCUS       DAZFDP010000594          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFDP010000594 DAZFDP010000000
VERSION     DAZFDP010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHJ1G_005873"
     CDS             complement(<1..176)
                     /locus_tag="ACHJ1G_005873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4767455.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYE"
     gene            complement(244..>479)
                     /locus_tag="ACHJ1G_005874"
     CDS             complement(244..>479)
                     /locus_tag="ACHJ1G_005874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFO4767456.1"
                     /translation="SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFS
                     VSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          123 a          127 c          107 g          122 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatgccc ttcaggtcat cgctgcctgg catatcagta
       61 tgggtctgaa gatacccggc gcttagtgtg gactgatgct ctgctgcgct cgctggcgta
      121 ccagcggcaa ccagccagac tactgcggac agaataacag cacataattt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
      241 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
      301 gatacttgct gtgggatacc agtcatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagt
//
LOCUS       DAZFDP010000595          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFDP010000595 DAZFDP010000000
VERSION     DAZFDP010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>476
                     /locus_tag="ACHJ1G_005875"
     rRNA            <1..>476
                     /locus_tag="ACHJ1G_005875"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFDP010000596          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFDP010000596 DAZFDP010000000
VERSION     DAZFDP010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>473)
                     /locus_tag="ACHJ1G_005876"
     CDS             complement(<1..>473)
                     /locus_tag="ACHJ1G_005876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4767457.1"
                     /translation="SGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRR
                     VYARFLDAVNFVAGNPEADPEQELRDRWVVEQMSELTAMTASFVLATPTETDGALFPG
                     RIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVAN"
BASE COUNT           93 a          177 c          117 g           86 t
ORIGIN      
        1 aaattagcca ccatgccgcg catctcacac ccgcgcatgc atttactgca tctgtccttc
       61 cggatatccg tggtggggtt gtcgaactca tccgccaccg caggaccgtg ataaccgcat
      121 tcatctcccc ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc
      181 gctccgtccg tctccgtcgg tgttgccagc acaaacgagg ccgtcatggc cgtcagctct
      241 gacatctgct ccaccaccca gcggtctctc agctcctgct ccgggtccgc ttccggattg
      301 cccgccacaa aattcaccgc atccagaaaa cgggcataca cccggcggcg gaccaccgtg
      361 gcccccacca ggctctgcag gtcctccgcc atcccggtga caaggccaaa cagattggac
      421 accgtcagcg acgggcgggc actgctgccc cggccgttca tctcaaagcc gct
//
LOCUS       DAZFDP010000597          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFDP010000597 DAZFDP010000000
VERSION     DAZFDP010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(177..404)
                     /locus_tag="ACHJ1G_005877"
     CDS             complement(177..404)
                     /locus_tag="ACHJ1G_005877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4767458.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          122 a           80 c          104 g          167 t
ORIGIN      
        1 ggaatctgtt caataaaaaa actgccgtgt tggagtcaca gcagtaatgt actgattggg
       61 tagaagatta ttattgttat gctttatttt tattctatat ggctgattat ttcaattcgg
      121 aattaataca gctaatgtct gtgatttttt ataaattcag caatataaag aaatagttat
      181 atgaacagcc atcgcagagc atactgtgta tcattctttt ttatagtcaa ctgacgggca
      241 tattttatgt ctgctgccag ctcccggcgg caagattcaa tgacccacgc agaaaaattt
      301 tctgaacctt tctggtcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg
      361 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
      421 acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttaccgg gca
//
LOCUS       DAZFDP010000598          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFDP010000598 DAZFDP010000000
VERSION     DAZFDP010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHJ1G_005878"
     CDS             <1..264
                     /locus_tag="ACHJ1G_005878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4767459.1"
                     /translation="FEQQIKNALLVASPVPGIRYQVEFDSADLLRATPGERFATYERG
                     IKSGVMCPNEAREREGLSPRDGGDEFSQAWKQEVKISEGEKPE"
     gene            261..>471
                     /locus_tag="ACHJ1G_005879"
     CDS             261..>471
                     /locus_tag="ACHJ1G_005879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4767460.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDIN"
BASE COUNT          130 a          113 c          147 g           81 t
ORIGIN      
        1 tttgagcagc agataaaaaa cgccctgctg gtggcctcgc ctgtacctgg tatccggtat
       61 caggtggagt ttgacagcgc ggacctgtta cgggccacac ctggcgaacg ctttgccacc
      121 tatgaacgcg gcatcaaatc cggcgttatg tgcccgaacg aagcccgcga acgtgaaggg
      181 ctgtccccgc gtgatggtgg tgatgagttc agccaggcat ggaaacagga agtaaaaatc
      241 agcgagggag aaaaaccgga atgaacatag ggcgactgcg tgacagggta acgattcaga
      301 ccctgaaaca gaccagggat ataaccggcg aaatactcga aacgtgggag gacggtcaca
      361 cactctgggc aagcgtgaac atgatcagca gcaaggaggc catttcatcg ggtgcagagc
      421 tggcgattgg taccgtaagg atctggatac ggtaccggaa ggacatcaac g
//
LOCUS       DAZFDP010000599          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFDP010000599 DAZFDP010000000
VERSION     DAZFDP010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>469
                     /locus_tag="ACHJ1G_005880"
     CDS             <1..>469
                     /locus_tag="ACHJ1G_005880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO4767461.1"
                     /translation="RIMATGHAHLDIAWMWPLREGRRKAIRTFATALTNIEKYPDYIF
                     GASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESLIRQIMHG
                     TRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINKFN"
BASE COUNT          159 a           93 c           89 g          128 t
ORIGIN      
        1 tcaggattat ggctactggt catgcccatt tagatatagc gtggatgtgg cctttaagag
       61 aaggacgcag aaaagcaata agaacatttg ccaccgccct gacaaacatt gaaaaatatc
      121 ctgattacat ttttggcgct agtcagtacc aactttttca ctggataaaa aaagattatc
      181 cctatttctt cgaaaagtta aaaaaacaaa tcgccgcagg ccgctttgag ttacaaggtt
      241 gcttttgggt cgaatgcgat ttaaatctgg taagtggtga atcgctgata aggcaaatca
      301 tgcatgggac acgattcaca ctgcagaatt tttcaaagaa gataaattat gtctggcaac
      361 cggatgtctt tggattcccc gccacgcttc cacaaattct caaaaaatca ggcattaact
      421 atattgcatc gcaaaagctc tcacaaaaca agattaataa atttaataa
//
LOCUS       DAZFDP010000600          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFDP010000600 DAZFDP010000000
VERSION     DAZFDP010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(124..>467)
                     /gene="escD"
                     /locus_tag="ACHJ1G_005881"
     CDS             complement(124..>467)
                     /gene="escD"
                     /locus_tag="ACHJ1G_005881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4767462.1"
                     /translation="VNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQNI
                     QPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISR
                     PDEYIHIPLSY"
BASE COUNT          136 a           89 c           88 g          154 t
ORIGIN      
        1 aaaatcaatg tctcatttcg gaataagttt ttcgtgtatc ttgtttgata gagaccttgt
       61 ttaccatatc tacaacatct gcacaataaa aaaccctccg aagaggggga agataataat
      121 aagttaataa gacaacggaa tatgtatata ttcgtctggg cgggagatgt tgataccatc
      181 tttaggatca atcgcgtcta tgacatagcc tcctttcaga atgctaccaa ttcccaggct
      241 tgtaccgtta tcaagagcaa ttgttggatt cttcatcgtg ccgctgattc tgagtatttt
      301 tccggggaaa atatctgctg aaatgtacgg ctgaatgttc tgaaatagta tcttgacgtt
      361 actatttttg ttcatcgcat ttataatggc aaggatctta ctctcgttct gttgagaaac
      421 ctcccccgca acaatgatga catcattctt tttgaaaata ttgacat
//
LOCUS       DAZFDP010000601          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFDP010000601 DAZFDP010000000
VERSION     DAZFDP010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>465
                     /locus_tag="ACHJ1G_005882"
     CDS             <1..>465
                     /locus_tag="ACHJ1G_005882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFO4767463.1"
                     /translation="LDSLDERGLILSLAAFSEDALGKMLLTFMLDNKASKELIEGFNA
                     PLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSHSWENISIEDQDISQQIKALS
                     FSRIDFECPKDNYQKIKKSISCLLIEIKITTSQIKKKHLKARLVGSNVNIGFS"
BASE COUNT          168 a           84 c           80 g          133 t
ORIGIN      
        1 ttggacagcc tagatgaaag aggcctaata ttgtctctag cggctttctc tgaagacgcc
       61 ttaggtaaaa tgcttttgac gttcatgctc gataacaaag cgtctaaaga attaatcgaa
      121 gggtttaatg ccccccttgg aacattttct tcacgaataa aagcctgttt ttccttggga
      181 ctaataacag agggtcaata caaagatctt gaacttctta gaaaaataag aaataaattc
      241 tctcacagct gggaaaacat ttcaattgag gatcaggata tttctcagca aattaaagcg
      301 ttaagcttca gtcgtattga ttttgaatgc ccaaaggata actatcaaaa aataaaaaaa
      361 tccatatcgt gtttactaat tgaaattaag ataaccacat ctcaaatcaa aaagaaacac
      421 ctcaaagcaa ggttagttgg ttcaaatgtt aacataggat tctcc
//
LOCUS       DAZFDP010000602          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFDP010000602 DAZFDP010000000
VERSION     DAZFDP010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>464)
                     /locus_tag="ACHJ1G_005883"
     CDS             complement(<1..>464)
                     /locus_tag="ACHJ1G_005883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767464.1"
                     /translation="SMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVIDH
                     IDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILRL
                     FDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIKKYEGEQLYLN"
BASE COUNT          161 a           86 c           62 g          155 t
ORIGIN      
        1 cattaagata cagctgttcg ccctcatact tcttaattaa ttgatagaag taccacttcg
       61 caaaatcagg ttgcttcatg aaaaaatcct tcaaaaaaat atcatttact aaaatgctga
      121 caggcacttc attaaatagc aacttaacat catcaaataa ccttagtatt aagcgtgatt
      181 tttcttgatt gcttaagtta aaatcattga catatacaac caacttctcc caaatcaatt
      241 cctgtaactc agggctgttt ttatagggtt ctttattgag gttgaaaaat aatgtctcag
      301 ctattttaga ctgatcgcag ttaaaatcaa tatgatcgat cacggagcgg gcatgcttta
      361 tctttgtagt atcatcagaa tattgcatac tttgattaaa acaccactcc agaattttta
      421 catcattaat atatggcaat tttaaaaaag tcttcaacat cgaa
//
LOCUS       DAZFDP010000603          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFDP010000603 DAZFDP010000000
VERSION     DAZFDP010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..197)
                     /locus_tag="ACHJ1G_005884"
     CDS             complement(15..197)
                     /locus_tag="ACHJ1G_005884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767465.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
BASE COUNT          152 a           98 c           70 g          142 t
ORIGIN      
        1 cagctacccc cgtattaatc agagttttca tttattttta atgcataatc agatgccgcc
       61 tcatcattat cctcagtatt atcatagccg aaaacttctc ccggacacgg gcgttcacga
      121 taatagtcat cgtcatagtt ttcatcgtca gataatgata tgtcgtattc tgaatatgat
      181 tcaccacaat tatacatgaa atcatctcct ttgggggggg caataattag tgagtttatt
      241 cgaaaaaatg atgagcgcca gaccaatcca cccaactttt gattataatc atgaagatac
      301 ttgtctcagt acaaattgaa tcaaactcaa tgcagcatga ttcaccttta tattctctca
      361 gggaaaccaa ataccactac tcaactatta agaacaaaat tcgggatgta tgattatcag
      421 aagatacctg catcctaaat aatttcagac agatattatc gt
//
LOCUS       DAZFDP010000604          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFDP010000604 DAZFDP010000000
VERSION     DAZFDP010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHJ1G_005885"
     CDS             complement(<1..348)
                     /locus_tag="ACHJ1G_005885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO4767466.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEA"
BASE COUNT          147 a          122 c           81 g          111 t
ORIGIN      
        1 agcctcaagc gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt
       61 gttctgaata ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct
      121 ctcaccaagg gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc
      181 gccattaccg ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt
      241 ttcaccaccg actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc
      301 caccccaaga ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc
      361 tattttaatt tgaactccag acttaaatag ctgtaacaaa catcccccat acatgacaac
      421 aaaaaccgga gccggactcc ggtttttgtg aagctgtcgg c
//
LOCUS       DAZFDP010000605          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFDP010000605 DAZFDP010000000
VERSION     DAZFDP010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>459)
                     /locus_tag="ACHJ1G_005886"
     CDS             complement(<1..>459)
                     /locus_tag="ACHJ1G_005886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4767467.1"
                     /translation="IYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGE
                     TARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSR
                     KAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLC"
BASE COUNT          135 a           99 c           75 g          150 t
ORIGIN      
        1 tgcataatgt attatctgaa tttgatggct caatattaat tgtagggatt cgcttacata
       61 cacctttaca tcccccgtca ttttcaatcc agagtaaatt tacccccgtt ctgtacagaa
      121 tatccagtac attttccgaa tttaccgctt tgcgggaatt gaactctttt cgcttcatat
      181 ctgaaaacat gcatggaaca gatatggctg tggatgtgcc acacgactgc acattatgaa
      241 aggatataag ttcattatat ttctttgtaa aatcattggt accacgagaa tatccattca
      301 gagcatagtt ttgacttctt gctgtctccc cgacaactaa aaacataatt accggctttt
      361 gtttttcatt tattgtgcgg gatgcatccg ttccagtctg acgaaactcg ccaggagaaa
      421 caaaaaaatc ccttacatat ttaaacccac tgtagatat
//
LOCUS       DAZFDP010000606          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFDP010000606 DAZFDP010000000
VERSION     DAZFDP010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..>457
                     /locus_tag="ACHJ1G_005887"
     CDS             85..>457
                     /locus_tag="ACHJ1G_005887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767468.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQL"
BASE COUNT          152 a           98 c           91 g          116 t
ORIGIN      
        1 agctgtccac atcggatatg tgacactaat aatatcaatg gattgatatt attaatggat
       61 ataaacatgc aataaggatt tatcatgaac attcaaccga tcgtaacatc cggaatcacc
      121 acacaaaaca atcgacatca tcacgcagaa caaacgtccc ctacacaaat accgcaatcc
      181 gaattaccta atggatgcga aacgggattt gttgttcata tcccagagga tatgcagcga
      241 catgcaccgg aatgcggtga aacaacagct ctactgagct tgataaaaga tgaaggtctg
      301 ctctctgggc tggataaata tcttgcacct catcttgaag aaggctctgc aggaaaaaaa
      361 gcattggata tgtttggttt attcaatgtc tctcagatgg cattagaaat acccagcacc
      421 gttccgggta tctctggtaa atatggtgtc cagctaa
//
LOCUS       DAZFDP010000607          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFDP010000607 DAZFDP010000000
VERSION     DAZFDP010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..89
                     /locus_tag="ACHJ1G_005888"
     CDS             <1..89
                     /locus_tag="ACHJ1G_005888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767469.1"
                     /translation="NICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            271..456
                     /locus_tag="ACHJ1G_005889"
     CDS             271..456
                     /locus_tag="ACHJ1G_005889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4767470.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
BASE COUNT          156 a           88 c           79 g          134 t
ORIGIN      
        1 tcaatatatg cccagaaaag gtagcagaat acctaagcga gttcagcgga aaacatttcc
       61 tcgatgtata taattattat tccaattgaa aaaaatataa agtatctatc acggcactct
      121 cagttgagta tcacaggcac atgtatttta gccccctgag tctacagaca accaatatta
      181 cttaaataag tgataatctt aatgctgttt tttagaacag atgattgata tttttgggcc
      241 ggagaaacgc agacaggctt tgttgaataa atcgagcttt tgctgagttt aaggatcaga
      301 tcactcattt tcctgcaaaa gctcattggg cgaaacaatc tgactcagag aacccggatt
      361 aaacgtctgg cccgcagaac catctatttc tcacgcactg tagtacgtaa taaaaaagtc
      421 atcggtactt ttatcaaaaa acatatattc tattaat
//
LOCUS       DAZFDP010000608          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFDP010000608 DAZFDP010000000
VERSION     DAZFDP010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHJ1G_005890"
     CDS             <1..>453
                     /locus_tag="ACHJ1G_005890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4767471.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFDP010000609          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFDP010000609 DAZFDP010000000
VERSION     DAZFDP010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHJ1G_005891"
     CDS             <1..>453
                     /locus_tag="ACHJ1G_005891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4767472.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gctgcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFDP010000610          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFDP010000610 DAZFDP010000000
VERSION     DAZFDP010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..>448)
                     /locus_tag="ACHJ1G_005892"
     CDS             complement(32..>448)
                     /locus_tag="ACHJ1G_005892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO4767473.1"
                     /translation="SRGRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKA
                     PAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYAT
                     IKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT           98 a          115 c          115 g          120 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggct
//
LOCUS       DAZFDP010000611          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFDP010000611 DAZFDP010000000
VERSION     DAZFDP010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>447
                     /locus_tag="ACHJ1G_005893"
     CDS             <1..>447
                     /locus_tag="ACHJ1G_005893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767474.1"
                     /translation="KRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAIISSNTSGNVIR
                     GDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDSLIEWKPSYYQLIKSEIQN
                     AIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRCRNIDFVFGN"
BASE COUNT          152 a           61 c           90 g          144 t
ORIGIN      
        1 taaaagatta atacatgatg atgctgtgtt ggaggatcaa aaggataaaa acttcattgc
       61 aaaaatgatt gcagaatatg ctgtgtctag aaatgcaatt atttctagca acacatctgg
      121 caatgtcatt cggggtgatg aaagcggagt aaaatggatt caatgtggta atatctttgt
      181 ctccctattt cataaggttc aagatgatca tgaaaacgat ggagatagga tttggcaaac
      241 tctcaatgat tctctcattg aatggaaacc atcttattat cagttaataa aatctgaaat
      301 tcagaatgca atcgaagctg aggctttatc ttttgtaaat catttggcta acgatcatta
      361 cggtcaagct gcgtggttaa atgagatatt aaaatcagac tcgcctgata ttagatgtag
      421 aaatattgac tttgtatttg gtaattt
//
LOCUS       DAZFDP010000612          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFDP010000612 DAZFDP010000000
VERSION     DAZFDP010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(195..>442)
                     /locus_tag="ACHJ1G_005894"
     CDS             complement(195..>442)
                     /locus_tag="ACHJ1G_005894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO4767475.1"
                     /translation="QESGCLSRLFSFLSGGVTKALESKLNERHITPLSPTWFPRTPLE
                     GVLTERGLSSLLRRVQSTENLDYSNAMISNSFSNSVV"
BASE COUNT          153 a          102 c           75 g          112 t
ORIGIN      
        1 agctggaagg aattcatcca ttcagctcaa tcccctcctc atcacagaat ctctgacttc
       61 tgccagccaa actcactctg aatattcatc ccatattttc ctaaaaacaa tgtattatcg
      121 acataaaaca aacagaaaaa ctccagaatc acaattattc tggagttaaa ccaggaggaa
      181 agctcaaatg ataatcacac tacggagtta gagaaagagt ttgatatcat agcattacta
      241 taatcaaggt tttcagtaga ctgtacgcga cgtaacaaag aagataatcc tctttccgtt
      301 aagaccccct ccagtggagt tcgaggaaac caggttggag aaagtggagt gatatgcctc
      361 tcatttagtt tactttccaa agccttagtc acaccgccag aaagaaagct aaataatctt
      421 gagagacacc cactttcctg ag
//
LOCUS       DAZFDP010000613          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFDP010000613 DAZFDP010000000
VERSION     DAZFDP010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..>434
                     /locus_tag="ACHJ1G_005895"
     CDS             26..>434
                     /locus_tag="ACHJ1G_005895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO4767476.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKTAAKKLNNKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDIPGSRV"
BASE COUNT          149 a           57 c           95 g          133 t
ORIGIN      
        1 tgaaataata attaaggaga ttatcgtgct gtcttactta atggcaattc actttgtttt
       61 atttgggaac tctactaatt taaaaaactt ctggaaatat gaagtaattc ggcggaaacg
      121 tatggatatc tggaggcttt taagagagaa aaaacagcgt aaccggaatt tccttttctg
      181 gtggcggttg gctaacgaaa tgtatattaa tggtaataaa ttacataaga cagcagccaa
      241 aaagttaaac aataaaataa ttaacaaatt tggttgtgaa attggattgg gcgcaaatat
      301 tggaaaaggg ttaacaattc cccatcatgc tggaattgtt gttcattttg ctgttgatgc
      361 tggtgaaaat ctggtgttac gacagaatac gaccattgga cagatagatg gtgacatacc
      421 tggttcaaga gtaa
//
LOCUS       DAZFDP010000614          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFDP010000614 DAZFDP010000000
VERSION     DAZFDP010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          124 a           89 c          102 g          116 t
ORIGIN      
        1 tgtcatgata tcgccgtcgt taacaggtaa ttcttctatg actccagcag acaggcgcag
       61 gcaggaggcg gtcacctgat tactgaacaa ccttctgact gacttcagcg tgtcaatcac
      121 aggctccacg tcatgcgtca gcatagtgct gatgctgacg gatgttacaa aaaatgcagc
      181 gtatggcgtt gagatagaaa gtctggtgct ggagataaat acacctacat catgataaaa
      241 aagggccagc gactgacctg aagaagacac tggctaaaag gctttgcatg tttgtagata
      301 cttatttttc acagacagca atgatgcctg tcaatatatt attaatatgc tgattgtttc
      361 agttacagat gctttattaa gggaaaatgt cacgatcggt gcaaaagtgg tttgaaccgc
      421 cccggttttc c
//
LOCUS       DAZFDP010000615          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFDP010000615 DAZFDP010000000
VERSION     DAZFDP010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          137 a           70 c           84 g          139 t
ORIGIN      
        1 cagataatta aataacaacc cttggtttgt gaaggcctcc gcaagtgttc gtttggcttc
       61 acatggcatc ttcttcttag aaaaatatcg acatattttg tgacacgaat tgcaaatctg
      121 gttttgttgt atggattgcg tgatttttga tctggtataa caggtataaa ggtgcaccaa
      181 gatagtcaat gagacagggc atctcgcaat ctatggcaaa catcacttca gttctttcac
      241 atcgggtgat gaaaatgcac ttcagtctga aaggaatatg aaaatgagat caacagacac
      301 tctattttat gactctgggt aaaatggatt gagtaagtga tatagcttac gaacattcaa
      361 atcaattaaa catcagaaga gattttatac tcaggtattt aatctggctc tctgtttatt
      421 taaataatgt
//
LOCUS       DAZFDP010000616          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFDP010000616 DAZFDP010000000
VERSION     DAZFDP010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>425
                     /locus_tag="ACHJ1G_005896"
     CDS             <1..>425
                     /locus_tag="ACHJ1G_005896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFO4767477.1"
                     /translation="ETIRHQYNTHTQDFGVTEWLLAAKSIGLKAKYVEKHFSRLSIIS
                     LPALIWRDDGKHYILSRVTKDSSRYLVYDPEQHQSLTFSRDEFEKLYQGKVILVTSRA
                     TVVGELAKFDFSWFIPSVVKYRRILLEVLTVSAFIQFLA"
BASE COUNT          124 a           69 c           88 g          144 t
ORIGIN      
        1 gaaactataa ggcatcagta taatacccac acacaagatt ttggggtgac tgaatggtta
       61 ctggcagcga aatccattgg cttaaaagca aaatatgtag aaaaacattt ttccagattg
      121 tcaataattt ctttacctgc gttgatatgg cgggatgacg gtaagcatta tatattgtct
      181 cgtgttacta aagattcatc acgctatctt gtttatgatc cagaacaaca tcagtcacta
      241 acttttagtc gggatgagtt tgaaaaactg tatcagggaa aagtcattct ggttacgtca
      301 agagcaacag tagtcggaga gttagctaaa tttgattttt cttggtttat cccctctgtt
      361 gtgaaataca ggaggatttt acttgaggtg ttaactgttt ctgcttttat tcagtttctt
      421 gcgtt
//
LOCUS       DAZFDP010000617          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFDP010000617 DAZFDP010000000
VERSION     DAZFDP010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHJ1G_005897"
     CDS             <1..330
                     /locus_tag="ACHJ1G_005897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4767478.1"
                     /translation="ATFSLRMKGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANG
                     GTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTV
                     TVSAAAG"
     gene            297..>424
                     /locus_tag="ACHJ1G_005898"
     CDS             297..>424
                     /locus_tag="ACHJ1G_005898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4767479.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          111 a           93 c          137 g           83 t
ORIGIN      
        1 gcaacgtttt cactgcgtat gaaaggcaaa ccggtgtcct ttgtggtacc gctggcgttt
       61 gtgaaaaatc tggataaaac acttaccgtg aatacaggtg cgctgctgac aatgtcagtc
      121 agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg tcagccggtt
      181 gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa
      241 tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc
      301 accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa aaggatctga
      361 aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat
      421 gggg
//
LOCUS       DAZFDP010000618          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFDP010000618 DAZFDP010000000
VERSION     DAZFDP010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>423)
                     /locus_tag="ACHJ1G_005899"
     CDS             complement(<1..>423)
                     /locus_tag="ACHJ1G_005899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO4767480.1"
                     /translation="IKERDKLSGIIHNIENSIISGHESSDIIRSHQHEWGDLSTVEQF
                     KKFEFYVKSELSFSKSIFDDIKTKYITDPETKRNALYHQLDSDIKERIAFLDISHYAY
                     PGSLLEKLQLSGYVFSDINIIAEYLLSSYGISGHYSHGV"
BASE COUNT          127 a           69 c           71 g          156 t
ORIGIN      
        1 cacgccgtga gaataatgcc cagaaattcc gtaactagac aataagtatt ctgcaattat
       61 gttgatgtca gaaaaaacgt agccgcttaa ctgtaatttt tctaataaac tacctggata
      121 tgcgtagtgg gatatgtcga gaaaggctat tcgctctttg atatcactat ctaattgatg
      181 gtaaagtgcg tttcgttttg tttcgggatc tgttatatat ttggttttta tatcatcaaa
      241 aattgacttg gaaaatgata actcactttt aacataaaat tcaaattttt taaattgctc
      301 taccgttgat aaatctcccc actcatgttg atggcttcgt attatatcag atgattcatg
      361 acctgatata atagaatttt cgatgttgtg tataatgcct gatagtttat ccctctcttt
      421 aat
//
LOCUS       DAZFDP010000619          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFDP010000619 DAZFDP010000000
VERSION     DAZFDP010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..296
                     /locus_tag="ACHJ1G_005900"
     CDS             <1..296
                     /locus_tag="ACHJ1G_005900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HFO4767481.1"
                     /translation="ANLSPSGGVLPLAATINGNVSVDENTGVMTPENGVSRNIRIAAG
                     IALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT          135 a           79 c           93 g          114 t
ORIGIN      
        1 cagcaaattt aagtccttct ggcggagtat taccactggc agccacaatc aatggaaatg
       61 tttcggtgga tgaaaataca ggagtaatga caccagaaaa tggggtaagt cgtaatatca
      121 gaattgcagc cggaattgca ctcgctacta cagctctggc tgctttaggt actggtatcg
      181 caatggcatg cacaacagat gcaagctccc aagaattctt aggtttaggt attgctaccg
      241 gtgtattagg tggtgttact gcgcttggcg gcggattagc gatgaaatat gcctaatcat
      301 cgcatcgcat aaataaaata gagaggtcat ggatgcatta tgccattacc tctctcatga
      361 aaagagacta accgtaaata tgaacaatat taatcaatca gaaaatatta atattcattt
      421 t
//
LOCUS       DAZFDP010000620          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFDP010000620 DAZFDP010000000
VERSION     DAZFDP010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..211
                     /gene="gpU"
                     /locus_tag="ACHJ1G_005901"
     CDS             <1..211
                     /gene="gpU"
                     /locus_tag="ACHJ1G_005901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4767482.1"
                     /translation="HIEVFLPAQVPDSELDSWMESRIYPAMSAIPALSGLITTMVTQG
                     YEYRRDDDMALWSSADLTYSITYEM"
     gene            219..>421
                     /locus_tag="ACHJ1G_005902"
     CDS             219..>421
                     /locus_tag="ACHJ1G_005902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4767483.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWT"
BASE COUNT          103 a           96 c          124 g           98 t
ORIGIN      
        1 gcatattgaa gttttcctgc ctgctcaggt gccggattca gagctggatt cgtggatgga
       61 aagccggatt tatccggcga tgagtgcgat cccggcactg tcaggcctga ttaccacgat
      121 ggttacgcag ggctatgagt atcgtcgtga tgacgatatg gcgttatgga gttctgctga
      181 tttgacttat tccattacat acgagatgtg aggacgatat gccaacacca aatcctctgg
      241 caccggtaaa aggtgccggt accaccctgt gggtttataa cggtcagggt gatgcctatg
      301 caaacccgtt gtcagacgat aactggcagc gactggcaca ggtaaaggat ctgacgccgg
      361 gcgagatgac ggcagaaccc tacgatgata actacctgga tgatgaagac gcggactgga
      421 c
//
LOCUS       DAZFDP010000621          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFDP010000621 DAZFDP010000000
VERSION     DAZFDP010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>419)
                     /locus_tag="ACHJ1G_005903"
     CDS             complement(<1..>419)
                     /locus_tag="ACHJ1G_005903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767484.1"
                     /translation="PLYEWLINKRLRYQYLTLLAFSILALLALYLLYRNTPKVSVNFF
                     EFYHKNLRGYLFSGFISVGSFLLSLHTFVIINLRDKVFATQEYKEIYSIATGIPIDKI
                     NDSVLYKPLDNLSSFINTSILCSITTAIAQFTIGLST"
BASE COUNT          152 a           59 c           79 g          129 t
ORIGIN      
        1 agttgaaagt ccaatagtga attgtgcaat tgctgttgtg atagaacata atattgatgt
       61 attaataaat gaggataaat tgtctaaagg tttataaagt acactatcat tgattttgtc
      121 aataggtata ccagtggcaa tgctataaat ttccttatat tcctgagttg caaaaacctt
      181 atcgcgtaaa tttattatga caaaggtatg caggctcaat aaaaatgagc ccacggaaat
      241 aaaaccggag aagagatagc ctcgtaagtt tttatgataa aactcaaaaa agttaacact
      301 tactttaggt gtgtttctgt acaataggta aagtgcaagc aatgccagga tcgagaatgc
      361 aagcagtgta aggtactgat acctcaatct tttatttatt agccattcat ataaaggca
//
LOCUS       DAZFDP010000622          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFDP010000622 DAZFDP010000000
VERSION     DAZFDP010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>419
                     /locus_tag="ACHJ1G_005904"
     CDS             <1..>419
                     /locus_tag="ACHJ1G_005904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4767485.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQL"
BASE COUNT           97 a           95 c          134 g           93 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagct
//
LOCUS       DAZFDP010000623          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFDP010000623 DAZFDP010000000
VERSION     DAZFDP010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>418
                     /locus_tag="ACHJ1G_005905"
     CDS             <1..>418
                     /locus_tag="ACHJ1G_005905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767486.1"
                     /translation="ESDVQGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMAAQ
                     AQAFEEKLASLSAVLQKHVTVDEVHPVLQAMVDDAVGTIPVPRDGRDYDPDVLQQAVN
                     DAVANIPVPADGKSITPDDVRPMLEQMVKEAVSHIPA"
BASE COUNT           98 a           90 c          140 g           90 t
ORIGIN      
        1 gaaagcgatg ttcaggggga tactgagaaa atgacggaac gggaactgtc cattattcgt
       61 gcactgggcg aagaattctc cacggtgctg gcggatttac agcgcacatt tgaggggaag
      121 atggccgcgc aggcacaagc gtttgaagag aaactggctt ccctgtcggc ggtattacag
      181 aagcatgtga cggtggatga ggtacatccg gttctgcagg cgatggtgga tgacgctgtg
      241 gggaccattc cggtaccgcg tgatggtcgt gattatgatc cggatgtact gcagcaggcg
      301 gtgaatgatg cggttgcgaa tattccggta ccggcggacg gcaaaagtat cacccccgat
      361 gatgtgcgtc cgatgcttga gcagatggtg aaagaggccg taagccatat ccctgctc
//
LOCUS       DAZFDP010000624          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFDP010000624 DAZFDP010000000
VERSION     DAZFDP010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /locus_tag="ACHJ1G_005906"
     CDS             <1..231
                     /locus_tag="ACHJ1G_005906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767487.1"
                     /translation="ISADIILTRTPHITEFSSDSITAQIKKNGINIQTGRMVSQKHLI
                     ITHDQEGKHLTTWYQQISLRVHSYKQHTEEVR"
BASE COUNT          159 a           98 c           63 g           97 t
ORIGIN      
        1 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
       61 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      121 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      181 atatcattac gtgtgcactc ttataaacaa cacacagaag aagtaagata attataaacg
      241 taaatttaat atctcccact gacatctccc ccacaataca ctgaaaaata ctcacagcag
      301 taaacacaga cacacctgat aatcatgcag ccgataaata ttttcccctt tacggagaaa
      361 tcagcctgct gtttaacaat aacggcgtat tttcatggaa ggagatcacc cagtaac
//
LOCUS       DAZFDP010000625          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFDP010000625 DAZFDP010000000
VERSION     DAZFDP010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>416
                     /locus_tag="ACHJ1G_005907"
     CDS             <1..>416
                     /locus_tag="ACHJ1G_005907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4767488.1"
                     /translation="HPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEV
                     VRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDE
                     LSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG"
BASE COUNT          108 a          115 c          114 g           79 t
ORIGIN      
        1 ccatcctgat gcggttgtat tcagccctaa aaagcgccag tggggaacgc aggatgattt
       61 gacctgcgca cagtggctct ggaaaaaaat catcgccctg tacgagcaag ccgccgaatg
      121 tgacggcgag gtggttcgtc ccaaagaacc gaactggaca gcctgggcaa acgaaattcg
      181 cctgatgtgt gtgcaggatg gtcgtactca caaacaaatc tgcgagatgt acagccgcgt
      241 cagccgcgat ccgttctggt gccgtaacgt gctcagcccg tcgaagttgc gggaaaaatg
      301 ggatgagctt tccctgcgct tatcaccgtc cgtcagcacg tacaccgaaa aacgcgaaga
      361 cccgtacttc aaatccagtt acgacaacgt ggactacagc cagatcccgg caggat
//
LOCUS       DAZFDP010000626          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFDP010000626 DAZFDP010000000
VERSION     DAZFDP010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHJ1G_005908"
     CDS             <1..330
                     /locus_tag="ACHJ1G_005908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4767489.1"
                     /translation="ATFSLRLKGKLVSYVVPLAFVKNLEKTLTVNTGALLTMSVSVNG
                     GTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQPQSITSDACTV
                     TVNGAGG"
     gene            336..>415
                     /locus_tag="ACHJ1G_005909"
     CDS             336..>415
                     /locus_tag="ACHJ1G_005909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4767490.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
BASE COUNT          111 a           89 c          127 g           88 t
ORIGIN      
        1 gcaacgtttt cacttcgcct gaagggtaaa ctggtgtcct atgtggtacc gctggcgttt
       61 gtgaaaaatc tggagaagac acttaccgtg aataccggtg cgctgctgac aatgtcagtc
      121 agtgtcaacg ggggaacgcc gccttataaa cacgcctgga agaaggatgg tcagccggta
      181 gagggacaga ctactgacac tttcagtaaa gccaatacgc agtcaggtga taagggggct
      241 tatacctgcg aggtaacgga ttctgcagaa cagccgcaga gcattacctc tgatgcgtgt
      301 acagtaacgg ttaatggtgc gggcggataa ggcttatggc aaaagatctg aaaacactgg
      361 cgctggccag actgtcgggg ttccgtcata aaacggtgaa ggtgccggaa tggag
//
LOCUS       DAZFDP010000627          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFDP010000627 DAZFDP010000000
VERSION     DAZFDP010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>414)
                     /locus_tag="ACHJ1G_005910"
     CDS             complement(<1..>414)
                     /locus_tag="ACHJ1G_005910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767491.1"
                     /translation="EAIVDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADGQYLAQA
                     KWDTPRVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNA
                     WGQQGDPASVSFRIAAPAAPSRIELTPGYFQITAVP"
BASE COUNT           65 a          145 c          119 g           85 t
ORIGIN      
        1 cgggaccgct gttatctgaa aatagcccgg tgtcagctca atccgagacg gcgctgccgg
       61 tgcggcaatc cggaacgata ccgatgccgg atcgccctgc tgtccccacg catttaccgc
      121 ccggactgtc aacctgtagt tccccagcgc cagttgcgtg aagcggtatg tggtttctgt
      181 cgtcctggcc gtgctgacca gccgctcact gccgtcatcc gctgccacgg tcaggcgaag
      241 caggaagctc acgcccttca ccacccgcgg cgtgtcccat ttcgcctgcg ccagatactg
      301 gccgtcagct gcgctcacct ccaccgtcag gtgctgcact gccggaggga tgacgctgtt
      361 cagggtgccg ctctggtcgc cgtcaaagtg cgctccgtta tccacgatgg cttc
//
LOCUS       DAZFDP010000628          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFDP010000628 DAZFDP010000000
VERSION     DAZFDP010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHJ1G_005911"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHJ1G_005911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZFDP010000629          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFDP010000629 DAZFDP010000000
VERSION     DAZFDP010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>413
                     /locus_tag="ACHJ1G_005912"
     CDS             <1..>413
                     /locus_tag="ACHJ1G_005912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4767492.1"
                     /translation="REYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQ
                     GRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKA"
BASE COUNT           98 a          119 c          137 g           59 t
ORIGIN      
        1 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
       61 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
      121 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
      181 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
      241 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
      301 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      361 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cgg
//
LOCUS       DAZFDP010000630          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFDP010000630 DAZFDP010000000
VERSION     DAZFDP010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>413)
                     /locus_tag="ACHJ1G_005913"
     CDS             complement(<1..>413)
                     /locus_tag="ACHJ1G_005913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4767493.1"
                     /translation="PDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTFLDGGGLH
                     FLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGAAAQSTTV
                     FHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAA"
BASE COUNT           86 a          121 c          115 g           91 t
ORIGIN      
        1 gcggctttgg tcatgacctc ctcaagggag gctgccagcg ctgcacgctt gttttcaaaa
       61 cttttaatct gttcgccaat attcattatg gtcttttcct tatgaaaaac ggttgttgac
      121 tgtgccgcag cgccggcaga agatgcgatt ttcaccaccg gtttccggtt gccggacgcg
      181 gcagaaaacg ggcggtcgta agatttaatg gtccggatgg tgcattccgc attcgcgggc
      241 acggtgacgg cagacacctc catcagttcc cagcgcagaa aatgcagtcc gcctccgtcc
      301 agaaaggtgt attcatgggg acggaagccc acggacagcc ccctgaccag cccggtctta
      361 atggccgccc agacctcatc cagccgggca gccagttgcg acggcatatc cgg
//
LOCUS       DAZFDP010000631          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFDP010000631 DAZFDP010000000
VERSION     DAZFDP010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>410
                     /locus_tag="ACHJ1G_005914"
     CDS             <1..>410
                     /locus_tag="ACHJ1G_005914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767494.1"
                     /translation="RDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRINVKDV
                     RGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFVSPIAL
                     NAPRDLASFRICSDFFDLLVGIKNNKLKIDFESY"
BASE COUNT          138 a           57 c           81 g          134 t
ORIGIN      
        1 tgagagattt aggtaatgta ggatataacg aaagccgcta tgcattcaat ttcaaagttg
       61 ataataatga ggattataag tctcttatta gtgccacgct tggatatccg accagaataa
      121 atgtaaaaga tgttcgcggt tgggatactc gatttgatat taattttcag atggaagaac
      181 tgacagctga acaagcaata atcgaaataa gatctgttga agcattctca acaggctcta
      241 taatattcga agatgaaaaa gaagatgtct cttttaagtg tgaatttttc gtttctccta
      301 ttgcacttaa tgctccaagg gatctggcaa gctttagaat ttgctcggac tttttcgatt
      361 tactggttgg tattaaaaat aacaaattga aaatagattt tgaatcatat
//
LOCUS       DAZFDP010000632          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFDP010000632 DAZFDP010000000
VERSION     DAZFDP010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>406
                     /locus_tag="ACHJ1G_005915"
     CDS             <1..>406
                     /locus_tag="ACHJ1G_005915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO4767495.1"
                     /translation="FDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGT
                     IWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSR
                     SLQIKYEALKHIEKNINDNIIYHPSNNANFYSV"
BASE COUNT          122 a           54 c           88 g          142 t
ORIGIN      
        1 gtttgataag gttattcaag aaggcttaag tggattgatt gagttaagta tcgatattaa
       61 tgatagtaca aatcatcgtg ataagtcttc tctttctcct gatgggagag ctgtgttaca
      121 ttttttaggt acaatttgga atatggcgat gggagctgta cctggttata atgcattgtc
      181 tggtgtaagt agcattttac atagtgctat agtaaaagaa tctagcaata tctgtgatta
      241 tattcagggg gctgtacgta ttggaatgga ctttgttcca ggcactcgct ctgacttaca
      301 tagccgttcg ctgcagataa aatatgaggc tttgaagcat atagaaaaaa acattaatga
      361 taatattatt tatcatccga gtaataatgc taatttctat tctgta
//
LOCUS       DAZFDP010000633          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFDP010000633 DAZFDP010000000
VERSION     DAZFDP010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          145 a           76 c           79 g          105 t
ORIGIN      
        1 ctggttcaga tgaaaggaaa ccaccgcaaa agcgtccggc ggcaggccgt ccacttccag
       61 tgtgaaacgt aatccggttg acatagcaac ctccggtaaa atgaataatg atccgtaccg
      121 tacaactgcg gataatgtga taagcgggac gacttcagac gttattaggg aaggaacaga
      181 aaatgctatg gttatgtgag aattttgttt aaatattact ttacagaaac gggtgtctgt
      241 tcagtcaaaa aaataatcac aagctcaacc tcttgtttct tggcgttatt caggacaggt
      301 acgcatatca caacgagtaa caaaaacgca aagttaaacc aatattcata atatattttt
      361 attcacagca ataaataatc caattaacat tattaaaatg aatga
//
LOCUS       DAZFDP010000634          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFDP010000634 DAZFDP010000000
VERSION     DAZFDP010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>403
                     /locus_tag="ACHJ1G_005916"
     CDS             <1..>403
                     /locus_tag="ACHJ1G_005916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO4767496.1"
                     /translation="KALAAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPEPVKV
                     TADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHE
                     SERNYQKAEQNSPNALQNEPETKQPEPVAQQE"
BASE COUNT          140 a          102 c           98 g           63 t
ORIGIN      
        1 aaggcactgg ctgccggggc tactgaaatc acgaaagaag aatatttcgc ccgcaaaaac
       61 ggtacatact caggttcaca acaaaatact ggtgcatctg acacgaccgc acaaccagag
      121 ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa tatcagccag
      181 cctgacgccg ataagttgct tgctgcctct cgcggagaat ttgttgcagg gattagcgac
      241 ccgaatgatc cgaaatgggt taaggggatc cagacccgcg attctgtaaa ccagaaccag
      301 catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca gcccaaatgc gttacaaaac
      361 gagccagaaa cgaaacagcc tgaaccagtg gcgcaacagg aag
//
LOCUS       DAZFDP010000635          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFDP010000635 DAZFDP010000000
VERSION     DAZFDP010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..383
                     /locus_tag="ACHJ1G_005917"
     CDS             <1..383
                     /locus_tag="ACHJ1G_005917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4767497.1"
                     /translation="LVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWN
                     GTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEEASLLTAWK
                     TYRVLLNRVDTTVASDVEWPVAPQ"
BASE COUNT          117 a           83 c          116 g           87 t
ORIGIN      
        1 atctcgttga agaccatcgg ggaaaaaccg tctatgacgt ggcttccggc gacgcgttat
       61 ttatttctga acttggccca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
      121 ttcagaagtg gaacggcaca gcctgggtga aagatgcaga agcagaaaaa ctgttccgga
      181 tccggaaggc ggaagaaaca aaaaacagcc tgatgcagat ggccagtgag catattgcgc
      241 cacttcagga tgccgtagat ttggatattg cgacggagga agaggcatcg ttactgactg
      301 catggaagac atatcgggta ttgttgaatc gtgttgatac aacagtagca tcggatgttg
      361 agtggccagt cgccccgcaa taaaaggata aagccatcga tag
//
LOCUS       DAZFDP010000636          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFDP010000636 DAZFDP010000000
VERSION     DAZFDP010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..371
                     /gene="ler"
                     /locus_tag="ACHJ1G_005918"
     CDS             <1..371
                     /gene="ler"
                     /locus_tag="ACHJ1G_005918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO4767498.1"
                     /translation="NMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQKQ
                     ITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGM
                     KKEDFLVKDTEDEIIPLKNI"
BASE COUNT          147 a           71 c           91 g           92 t
ORIGIN      
        1 tgaatatgga aactaattcg cacacaacaa gcccatacat tcagcttatt gagcaaattg
       61 aagtgttaca acaggaagca aagcgactgc gagagcagga aattcaaagt gtaattgagt
      121 cgattcaaaa gcagattact tattacaata taaccctaca agagctggga tatactaatg
      181 tgcctgatga tggccttgct cgccggaact catcgaaagg agtttattat cgcaatgaag
      241 aagggcagac ctggtcggga gttggccgac agccacgctg gcttaaagaa gcactgttga
      301 atggaatgaa gaaagaagat tttcttgtga aggacaccga agacgaaata ataccgctga
      361 aaaatattta acatgaaatc attaaatgat aacgataact g
//
LOCUS       DAZFDP010000637          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFDP010000637 DAZFDP010000000
VERSION     DAZFDP010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>393
                     /locus_tag="ACHJ1G_005919"
     CDS             <1..>393
                     /locus_tag="ACHJ1G_005919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO4767499.1"
                     /translation="TQRIITMSNELGNNRLKNSQLEILELKDPVLTEGILYQRRISSL
                     LITTEYHSLALQQVSSFWRVTDPNFGHCDFHSLAQALTFIKNITSNGNFSSLYGSGII
                     KIYFSESPNNWKYIKLPLIQNNSLSRDIY"
BASE COUNT          137 a           68 c           58 g          130 t
ORIGIN      
        1 acacaacgta tcattacaat gtccaatgag ttaggcaata atagattaaa aaactcacag
       61 ttagaaatcc tagaattgaa ggaccctgta ctaactgaag gtatattata ccagcgtcga
      121 atatcatctc ttttaataac aacagaatat cactcactcg cgttacaaca agtatcttct
      181 ttttggagag taaccgatcc taattttggg cattgtgact tccattcctt agcacaggct
      241 ttaacgttta taaaaaatat aacgagcaat ggtaactttt catctttata tggaagtggt
      301 attattaaaa tttactttag tgagtctcct aataattgga agtatataaa actccctctt
      361 attcagaata attctttgtc aagggatata tat
//
LOCUS       DAZFDP010000638          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFDP010000638 DAZFDP010000000
VERSION     DAZFDP010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..285)
                     /locus_tag="ACHJ1G_005920"
     CDS             complement(130..285)
                     /locus_tag="ACHJ1G_005920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4767500.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(294..>392)
                     /locus_tag="ACHJ1G_005921"
     CDS             complement(294..>392)
                     /locus_tag="ACHJ1G_005921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4767501.1"
                     /translation="MASRDFIQLCVVAVNFLSGADSGGKNEQATEA"
BASE COUNT           90 a          123 c           96 g           83 t
ORIGIN      
        1 gcctgacgct cgagattttt gatcgccccc tgcgttcgct ggatggagtc accaaactgc
       61 cccatcaggc ggcgggcgtt ttcggcaggc cgggtcagcc tgtcaacggc gctgaaagcg
      121 acccggatat caagagtctt cattgtctgc attcccgctg cgaagtgccg cccgctcacg
      181 ccagctaacc acttcgccgg gcgtcatcat gaagatttcg gcgggcgacc agttaaaaat
      241 aacggcaata tctgccacaa agtcttctat gtgctcaaag cacacaaccg tgatcaggct
      301 tccgtcgcct gttcgttctt cccgccagag tccgcaccgc tcaaaaaatt tacggcaacc
      361 acacataact gaataaagtc acgggatgcc at
//
LOCUS       DAZFDP010000639          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFDP010000639 DAZFDP010000000
VERSION     DAZFDP010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>390
                     /locus_tag="ACHJ1G_005922"
     CDS             <1..>390
                     /locus_tag="ACHJ1G_005922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767502.1"
                     /translation="GVDVDEWMRSLKRHERRALMRSLRDKQAKNSSDEMQSWTQSRKQ
                     QRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYVDGSIIAPQGFEIVRKP
                     DTRPDSRITQLWQRLSRNHGVSSTEIRH"
BASE COUNT          103 a          104 c          114 g           69 t
ORIGIN      
        1 ggcgttgatg tggatgaatg gatgcgctca ctgaaacggc acgaacgccg ggcgctgatg
       61 cgttcgcttc gtgacaaaca ggcaaaaaac agcagtgatg aaatgcagag ctggacacag
      121 agccgcaaac agcagcggcc tttgcctgat aaccacgaat tactcgctaa agaatggcgg
      181 gagtctgctg aatctctcgg cctgcatatc ggtgaacaac agatgcagca cctgttacgg
      241 ggcggcagtc tgtacgttga cggcagcatc attgcaccgc agggatttga aattgtacgc
      301 aaaccggata cccgcccgga cagccgaatc acgcagctct ggcagcgcct gagccgtaat
      361 catggcgtaa gcagcacgga gatccgccat
//
LOCUS       DAZFDP010000640          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFDP010000640 DAZFDP010000000
VERSION     DAZFDP010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          120 a           72 c           92 g          106 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg
//
LOCUS       DAZFDP010000641          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFDP010000641 DAZFDP010000000
VERSION     DAZFDP010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..320)
                     /gene="bhsA"
                     /locus_tag="ACHJ1G_005923"
                     /gene_synonym="ycfR"
     CDS             complement(63..320)
                     /gene="bhsA"
                     /locus_tag="ACHJ1G_005923"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4767503.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          101 a           86 c           94 g          107 t
ORIGIN      
        1 ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat gagggttaat
       61 gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac agaagtaata
      121 cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc cagcgatccc
      181 agatttgtcc ccgcgttagc actgattgta ccgacttttt gttggccttc tggcgttgac
      241 tgaacttcga cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg
      301 agggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt
      361 acttaacgat cgataactaa atgataga
//
LOCUS       DAZFDP010000642          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFDP010000642 DAZFDP010000000
VERSION     DAZFDP010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..290)
                     /gene="fumD"
                     /locus_tag="ACHJ1G_005924"
     CDS             complement(81..290)
                     /gene="fumD"
                     /locus_tag="ACHJ1G_005924"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4767504.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          113 a           96 c           60 g          117 t
ORIGIN      
        1 agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca ggatgaagct
       61 taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc catcgcctgc
      121 ttttccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa tacgcctgcg
      181 ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac tttaccgaca
      241 actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat tctttatacc
      301 tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa aatcaaacaa
      361 aatcagacaa ataacgcgat aaatta
//
LOCUS       DAZFDP010000643          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFDP010000643 DAZFDP010000000
VERSION     DAZFDP010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>383)
                     /locus_tag="ACHJ1G_005925"
     CDS             complement(<1..>383)
                     /locus_tag="ACHJ1G_005925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767505.1"
                     /translation="LGDLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELSD
                     INKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQ
                     GGQCKPEYGEERFCSELIKYGAGNL"
BASE COUNT          118 a           84 c           51 g          130 t
ORIGIN      
        1 caagatttcc tgcaccgtat tttatcagtt cagaacaaaa ccgttcttcg ccatattctg
       61 gtttacattg ccctccctgc caatagaatg ataactgctt cacattttga tatcctatct
      121 ctctgatcag agtttctcga tataatcgtg aaaaaatata gtcagggcat tgaatttcaa
      181 ataatgcact acttagagca agcaatctta ttttcccaat tgactgatat aaaaaacctt
      241 ccttatttat atcagataat tccactccag aaattttatc ttgtatttcc tgacgcaata
      301 tcttgttgag ttgattacga gtagaactac tttctcttaa atgccgaaaa ctaacctgat
      361 tgacaccggt taaatcacct agc
//
LOCUS       DAZFDP010000644          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFDP010000644 DAZFDP010000000
VERSION     DAZFDP010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..>382
                     /locus_tag="ACHJ1G_005926"
     CDS             182..>382
                     /locus_tag="ACHJ1G_005926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO4767506.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNT"
BASE COUNT          131 a           62 c           76 g          113 t
ORIGIN      
        1 ctgtattcag ggttacgatt ctatacaata gaacagggag gggaaaggat gtcatcgtta
       61 atgagacttt ttgttaatgt caacaatgct atgaatttat tttagaaaaa caattaagct
      121 gacgtagtgt tccgtctaaa tgacggggcg atattaacat gattagaaca agaggaattt
      181 tatgttatct tcattaaatg tccttcaatc cagcttcaga ggaaagacag ccttatcaaa
      241 tagtacactt ctccagaaag tttcttttgc tggaaaagaa tatcctctgg aacctattga
      301 tgaaaaaacc cctattcttt ttcagtggtt tgaagcaagg ccagagcgat acgaaaaagg
      361 agaagtacca atattgaata cc
//
LOCUS       DAZFDP010000645          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFDP010000645 DAZFDP010000000
VERSION     DAZFDP010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..333)
                     /locus_tag="ACHJ1G_005927"
     CDS             complement(109..333)
                     /locus_tag="ACHJ1G_005927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767507.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          101 a           96 c           77 g          105 t
ORIGIN      
        1 gtttttgatt atccccgcac actctcgcag aggagtctcc ctgtcgggct gcggtctctg
       61 ttaatgcagg aatacggcga caataccgcg catggataat aaggtcgctc aacacactgg
      121 ctgtaattca gcggatacca ttcggcattt atcagtattc atcacacact caacggtgaa
      181 ttcttcatgc gtggcattca cttcatatgt tcgtgaataa cattcagtgc atttacctct
      241 gaacacctct tcaagcagaa cacggccatg ttgcaaaaca cggaacggaa ttgttccctg
      301 aaaaggtttt accgttacct gtaatttctt catacatcct ccggataata aaaagcctgc
      361 ttagtacact gagtgcgga
//
LOCUS       DAZFDP010000646          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFDP010000646 DAZFDP010000000
VERSION     DAZFDP010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>374)
                     /locus_tag="ACHJ1G_005928"
     CDS             complement(<1..>374)
                     /locus_tag="ACHJ1G_005928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767508.1"
                     /translation="RRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNT
                     YRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFD
                     GVYEFIRNIRKNSTPGKTKLIA"
BASE COUNT          111 a           59 c           70 g          134 t
ORIGIN      
        1 tgcgataagt tttgtttttc ctggtgtact gtttttgcga atattacgaa tgaattcata
       61 tacgccatca aagatatctt tgaaatgcgc attcgctggc gtgtatccgg ataaacataa
      121 cttaacgtaa tttcgcagat atgtccttgc agcatgatat atatcatcct cattatccgg
      181 tatctgagtg agaaaagaat gaatatgata gtaaaatgcc atctcattat ccgggcgata
      241 tgtgttcctg atgtcgagtg cgagaatcat tggtacaatg tcattaaaat atttttgttt
      301 tattatttga tgtgttttgt ctgtaaaaac agaatagtgc attagcttaa catagagatc
      361 ttcatatctc ctaa
//
LOCUS       DAZFDP010000647          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFDP010000647 DAZFDP010000000
VERSION     DAZFDP010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..327
                     /gene="espW"
                     /locus_tag="ACHJ1G_005929"
     CDS             <1..327
                     /gene="espW"
                     /locus_tag="ACHJ1G_005929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO4767509.1"
                     /translation="SISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNI
                     KTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTW
                     DPLVRN"
BASE COUNT          143 a           77 c           59 g           93 t
ORIGIN      
        1 tcaatctcat taatagagac aaaacagagt ttgttgctac atcgattata ttcagatgaa
       61 gtacttcaga gaaatggaac acttatcccg acaccactac atgaagaaaa atcaattcca
      121 gctgacaata taaaaacaat gctcaacaac ataccgactt acaaaatgtt accgccattc
      181 acagaaacac aaggtaattg ttcttctggc gcagccacgt ttttacgcaa atcaggcgcc
      241 gaagaaaaag atattcttgc atgtagcccc cgaaattatg ggctgcatca taacataaaa
      301 acatgggacc ccttggttag aaattaagga tccaaatatt atatatttca tataaagcaa
      361 ggaaaatatt gc
//
LOCUS       DAZFDP010000648          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFDP010000648 DAZFDP010000000
VERSION     DAZFDP010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            168..>367
                     /locus_tag="ACHJ1G_005930"
     CDS             168..>367
                     /locus_tag="ACHJ1G_005930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4767510.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQ"
BASE COUNT          144 a           76 c           58 g           89 t
ORIGIN      
        1 taataattat catctacatg agatttatca cgacatgtgt gtacgccatc aaaccacgag
       61 aaataatcaa ttattacgca ggtatcgtat taattgatct gcatcaaatt agcgtaaaag
      121 caacttcaga taatacaaat cagcaacact gaatacgggg caacattatg tcatcaaaga
      181 acagaacccg cagaacaaca acccgcaaca tccgatttcc aaaccagata attgaacaaa
      241 ttaacatcgc tcttgacctg aaaggttcag gtaatttttc agcgtgggtt attgaagcct
      301 gcagaagaag attaattaat gaaaaatatt ctcaatttgt acccaacaaa gacaaacacg
      361 accagag
//
LOCUS       DAZFDP010000649          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFDP010000649 DAZFDP010000000
VERSION     DAZFDP010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..211)
                     /locus_tag="ACHJ1G_005931"
     CDS             complement(<1..211)
                     /locus_tag="ACHJ1G_005931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO4767511.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGK"
BASE COUNT           99 a           74 c           66 g          123 t
ORIGIN      
        1 ttttccccgt tgtgactctt gaaacatttt ggccaagaat attaagagta tagtcattaa
       61 cggatttcat tcctaaaaaa acaagtaatc cttctttctc ttgcgctttt tcgattgttc
      121 tccctgctaa agttattggg atggattgag attgttttgt cggaactgat acgctggcgc
      181 taataggtag tagggttctt atgctaaaca tacaacctct ttcttaattt tcgaacctgt
      241 ttaggattct gtgtaaattc aaaataaacc tgtctgaaca ttcctaacaa acatccaccg
      301 gacatgacaa caaaaaccgg agccggactc cggtttttgt gaagctgtcg gctatttcat
      361 tc
//
LOCUS       DAZFDP010000650        30104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.043, whole genome shotgun sequence.
ACCESSION   DAZFDP010000650 DAZFDP010000000
VERSION     DAZFDP010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.043"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..975)
                     /locus_tag="ACHJ1G_005932"
     CDS             complement(46..975)
                     /locus_tag="ACHJ1G_005932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO4767512.1"
                     /translation="MSFIECYEPEYVRKFLARPPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            complement(1102..1530)
                     /gene="flgN"
                     /locus_tag="ACHJ1G_005933"
     CDS             complement(1102..1530)
                     /gene="flgN"
                     /locus_tag="ACHJ1G_005933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO4767513.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            complement(1543..1821)
                     /gene="flgM"
                     /locus_tag="ACHJ1G_005934"
     CDS             complement(1543..1821)
                     /gene="flgM"
                     /locus_tag="ACHJ1G_005934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4767514.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            complement(1902..2639)
                     /gene="flgA"
                     /locus_tag="ACHJ1G_005935"
     CDS             complement(1902..2639)
                     /gene="flgA"
                     /locus_tag="ACHJ1G_005935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4767515.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            2721..3056
                     /locus_tag="ACHJ1G_005936"
     CDS             2721..3056
                     /locus_tag="ACHJ1G_005936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO4767516.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            3059..3490
                     /gene="flgC"
                     /locus_tag="ACHJ1G_005937"
     CDS             3059..3490
                     /gene="flgC"
                     /locus_tag="ACHJ1G_005937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4767517.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            3490..4203
                     /locus_tag="ACHJ1G_005938"
     CDS             3490..4203
                     /locus_tag="ACHJ1G_005938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO4767518.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            4227..5429
                     /gene="flgE"
                     /locus_tag="ACHJ1G_005939"
     CDS             4227..5429
                     /gene="flgE"
                     /locus_tag="ACHJ1G_005939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4767519.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            5429..6166
                     /locus_tag="ACHJ1G_005940"
     CDS             5429..6166
                     /locus_tag="ACHJ1G_005940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO4767520.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            6245..7030
                     /gene="flgG"
                     /locus_tag="ACHJ1G_005941"
     CDS             6245..7030
                     /gene="flgG"
                     /locus_tag="ACHJ1G_005941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4767521.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            7113..7778
                     /gene="flgH"
                     /locus_tag="ACHJ1G_005942"
     CDS             7113..7778
                     /gene="flgH"
                     /locus_tag="ACHJ1G_005942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4767522.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            7793..8893
                     /locus_tag="ACHJ1G_005943"
     CDS             7793..8893
                     /locus_tag="ACHJ1G_005943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4767523.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            8893..9192
                     /locus_tag="ACHJ1G_005944"
     CDS             8893..9192
                     /locus_tag="ACHJ1G_005944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO4767524.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            9374..10750
                     /gene="flgK"
                     /locus_tag="ACHJ1G_005945"
     CDS             9374..10750
                     /gene="flgK"
                     /locus_tag="ACHJ1G_005945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4767525.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGISMEEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            10765..11694
                     /gene="flgL"
                     /locus_tag="ACHJ1G_005946"
     CDS             10765..11694
                     /gene="flgL"
                     /locus_tag="ACHJ1G_005946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4767526.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            11711..12688
                     /locus_tag="ACHJ1G_005947"
     CDS             11711..12688
                     /locus_tag="ACHJ1G_005947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO4767527.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(12743..13585)
                     /locus_tag="ACHJ1G_005948"
     CDS             complement(12743..13585)
                     /locus_tag="ACHJ1G_005948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO4767528.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            14071..14985
                     /gene="lafA"
                     /locus_tag="ACHJ1G_005949"
     CDS             14071..14985
                     /gene="lafA"
                     /locus_tag="ACHJ1G_005949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO4767529.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            15192..16508
                     /gene="fliD"
                     /locus_tag="ACHJ1G_005950"
     CDS             15192..16508
                     /gene="fliD"
                     /locus_tag="ACHJ1G_005950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4767530.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            16531..16923
                     /gene="fliS"
                     /locus_tag="ACHJ1G_005951"
     CDS             16531..16923
                     /gene="fliS"
                     /locus_tag="ACHJ1G_005951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4767531.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            16928..17239
                     /locus_tag="ACHJ1G_005952"
     CDS             16928..17239
                     /locus_tag="ACHJ1G_005952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO4767532.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            17236..18297
                     /locus_tag="ACHJ1G_005953"
     CDS             17236..18297
                     /locus_tag="ACHJ1G_005953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4767533.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            18305..18772
                     /locus_tag="ACHJ1G_005954"
     CDS             18305..18772
                     /locus_tag="ACHJ1G_005954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO4767534.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            18792..19508
                     /locus_tag="ACHJ1G_005955"
     CDS             18792..19508
                     /locus_tag="ACHJ1G_005955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO4767535.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            19521..20384
                     /gene="motA"
                     /locus_tag="ACHJ1G_005956"
     CDS             19521..20384
                     /gene="motA"
                     /locus_tag="ACHJ1G_005956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4767536.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            20387..21310
                     /gene="lafU"
                     /locus_tag="ACHJ1G_005957"
     CDS             20387..21310
                     /gene="lafU"
                     /locus_tag="ACHJ1G_005957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4767537.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            21381..22436
                     /gene="dinB"
                     /locus_tag="ACHJ1G_005958"
     CDS             21381..22436
                     /gene="dinB"
                     /locus_tag="ACHJ1G_005958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4767538.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            22433..22885
                     /locus_tag="ACHJ1G_005959"
     CDS             22433..22885
                     /locus_tag="ACHJ1G_005959"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4767539.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            23131..24270
                     /locus_tag="ACHJ1G_005960"
     CDS             23131..24270
                     /locus_tag="ACHJ1G_005960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4767540.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            24267..24881
                     /gene="prfH"
                     /locus_tag="ACHJ1G_005961"
     CDS             24267..24881
                     /gene="prfH"
                     /locus_tag="ACHJ1G_005961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4767541.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(24938..26395)
                     /gene="pepD"
                     /locus_tag="ACHJ1G_005962"
     CDS             complement(24938..26395)
                     /gene="pepD"
                     /locus_tag="ACHJ1G_005962"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4767542.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            26656..27114
                     /gene="gpt"
                     /locus_tag="ACHJ1G_005963"
     CDS             26656..27114
                     /gene="gpt"
                     /locus_tag="ACHJ1G_005963"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4767543.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            27206..28450
                     /gene="frsA"
                     /locus_tag="ACHJ1G_005964"
     CDS             27206..28450
                     /gene="frsA"
                     /locus_tag="ACHJ1G_005964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4767544.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            28508..28909
                     /gene="crl"
                     /locus_tag="ACHJ1G_005965"
     CDS             28508..28909
                     /gene="crl"
                     /locus_tag="ACHJ1G_005965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4767545.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(28948..30003)
                     /gene="phoE"
                     /locus_tag="ACHJ1G_005966"
     CDS             complement(28948..30003)
                     /gene="phoE"
                     /locus_tag="ACHJ1G_005966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4767546.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         7366 a         7858 c         8237 g         6643 t
ORIGIN      
        1 ttggggagag ggttagggtg aggggcaaag cgcgcgcaga gcgactcacg ttagcagcga
       61 aaacgctgaa agcagcgggt cggcggcaca taacgactga atttgctggc gaaccaacag
      121 cgcattttca ctggctcgcc agcgttcaaa catggcaaag agggcgaaaa tatcctcctg
      181 ccgcagcgga gaaccatcca gcgtccataa actgattaac gtgcggagga gataaaaatc
      241 gcacaccagc gggaaataat ccgccccgtc agtctgccca atcatatcgt ggtaaacgcg
      301 gtaaatcagc cagttacgca tcatccaggg cgcggtggca aaatgctgtt gccaggtggt
      361 ttgccactgc gtctgaagct gctgttgtaa cagggcttcg ctgttggcgt catgcagcgt
      421 cagtaaagta acctgcaacg gcagcgaggc tttgcgcggg ctttccggca aaatcgacca
      481 gtcaaacgca caaccgccga gcagcgtcat caactggcgc gccagctgtg gcacgggcgg
      541 gagctgggcg tactgtgcct gtagcgtacc ttttttcgca tgatcggcca gcgcctgtgg
      601 cagcgtcgtc aaacgggcca ggatgtctcc gtctctgccc ggcattttgc tggcgcagga
      661 gagcaaaatc ccgacagcat acagggaaag ctccggggtt aaatcacgga gcgtgagggt
      721 actcaaaatc acctggttta ctatcgcatc gcgcgcggaa agctctgcgg aattatcttc
      781 cacagggcgg taggtgagct gtaaatcaaa ggccctgaca tcgttgagca ccgcttcgca
      841 gctggcaata tcggtcaatg acaggctcag gcgaatctcg tttttccaca ccttgcggac
      901 gtataaaggc gaaccggggg gacgcgctaa aaatttgcgc acgtattccg gttcgtaaca
      961 ttcaataaaa ctcataggtg gcttccagtc tttaatgctg tgcaggatat gcaataagcg
     1021 tgccggggat ttttgtaatt aaaaacaagg gattggaaat caggcggaaa atccatttcc
     1081 gctatgggga aggtattcgc gttattcctg atagagaaaa tctgcttcgc tgcctaaaaa
     1141 cgactgaata aattcatact ggcgaatcaa cagctcgccg tttttgtcgt tgtaggcttt
     1201 gcagcgttcc gctttttgct caaggcgttg ccacaactgt tgtgcagccg ctttttgaac
     1261 agccggaagc caggtgaaaa cctgcgccag accgtcacga tttacgctaa caccgagcat
     1321 tttcagcagg ctatgccgct gatggctatg tccatgcaac tgctcatagt gatgggttat
     1381 ctcatcgttt accgccagca actcttcgct ggcgcggcga atcatgcaga tccgctgacg
     1441 ctggagaagt tcgcgcaggg aatcatacag cgcgtcatcg tcacgaatgc cctgcaacaa
     1501 cgccttcacc tgttgtaacc ggatactcac ttcatcggcc tcttagttct ggaaaaaact
     1561 cagcatgtcg ctggctaact gttcagtgtc cacttgcggg ctgccggacg ccagcatcgc
     1621 ctgcacttgc gccactttgt tgctgtcgat atcgctctct tgctggtcgt tcagcgtttg
     1681 ctgggcagac tgcaatcccg cctgagtaat gtcgtcggcg ctcagcgtcg tggtggtggt
     1741 caccgtggtt ttgcgcggct ggctggagcc tgccgttgtg gctgtcgggc gattgccagg
     1801 catagtcggg gtgattttca tcgcaacctc gtctcggtat ttgggtgggt tactgcggta
     1861 aagcggttaa caggcggcaa aactttaatc gcctgcgcaa attactccgc gcttaccgtt
     1921 gtcaccacgc cgctttccgc caccatcgcg ctgatgaccc gctggctgct ggcattgcgc
     1981 actttcacca tctcgccctt acgaccattt ttcagcgcca cgccgggcat cgaggcggtg
     2041 atccccgcgc tctggtaaac aatcatcacc ggctgatcgc gcccgaccag caccggagac
     2101 accagttctt ctttggtgat gggtctgcct ggttgcaatg cgtgcttact gcttaaacca
     2161 atcgcgtcat caagctccat caacacatcg cggcgctggg cgctgacgtt gtagggctta
     2221 agctccacat cattcgcggt gagcggcata tcgcgggcaa ccagtgactt cgtcattacc
     2281 accggcacca gtacatccgg gcgcaccgcc acgttcatct gccagctttg cgggcaacta
     2341 acggtgaagt tcatccggtt gagggcgatt tccgcaggcg aagtcagcgt gacgccgggt
     2401 gttttagtac agggcgcggc ggtcgcaatc tgcgagggaa tgtagatctt aaaggtgtag
     2461 cgatagtcat gccactgccg ctgctgcgcc agcgattcaa ttttttggct cgcggcattc
     2521 agcacctggg cgttgacttg ttcccgcgcg ctgtgttgca cgggatggac ggcggcgtag
     2581 cccggtaagg ccagcgccag gaggaagtaa aacttccgcc tgcaaaattg aaaactcata
     2641 aaccctttct catcagtgac ttaaaaaatg gcatggcgat tgcttagcgt atgcggattg
     2701 aagcaggagt gatgcaggaa atggggatca gttttcagca ggcattggga gtacatccgc
     2761 aggcagtgaa gttacgtctt gagaggaccg agctactgac tgcgaatctg gcgaacgtcg
     2821 atacgccaaa tttcaaggct aaagatattg attttgccag ggagatgcaa cgggcaaata
     2881 acgcggcggt ggatgtccag taccgcgtgc cgatgcagcc gtcggaagat ggcaacaccg
     2941 tggaactgaa cagcgagcag gcgcggtttt cacaaaatag tatggattat caaagcagtt
     3001 tgacctttct gaatctgcaa atcagcggca tcagagaggc cattgaaggg aaataagcat
     3061 gtcatttact gatatctatc agatttccgg ctcggcgatg acggcgcaaa cgctacgtct
     3121 gaataccgtt gccagtaacc tggcgaatgc caatgcgccc gccagcagcg aggcgcaggc
     3181 gtataaagcg cgcagcccgg tgtttgccgc ggtgtatcac cacagcctgc tggcgggtac
     3241 acatcgtcat gccattgacg gtgccagcgt gcaggtacag gacgtgctgc aaaccggcgg
     3301 ggcggtgaaa cgctatgaac cgcattctcc gctcgccgac gccaacggtg acgtctggta
     3361 tcccgacgtc aacgtggtgg agcagatggc ggacatgatg tcggcctcgc gcgatttcga
     3421 aaccaacgtc gatgtgctca acaacgtgaa aagtatgcag caaagtttgc tgaaactggg
     3481 agaagcctga tgaataccct ggcgctgaat gcgcaatcgc cgcagccgac cgccacggcg
     3541 gaaagcaata atgtggcggc cagcgacacg caaagcagca gcgatagctc gcctgtcgat
     3601 accttcctga cgctgtttgt cgccgagatc caaaaccagg acccgaccga cccgaccgac
     3661 gctaccgaat acatcgacca gctgtcgtcg atggcgcagg tggcgatgat ggaagagatg
     3721 agcgtgcagg ccaacaccaa cgcggtgctg atgagcaaca ttcaggtgat ggcgctcggt
     3781 aacctggtgg gcgacgacat tatggtgcag accaccgcct tgcaggtgag cgaccagacc
     3841 attaacggtc gcgctacgct ggacgacgcc tgtaccaccg ccgatctcca ctttaccgat
     3901 gccgcaggcg atgactacac cgtctcgctg atcccagaag gcagttcgtc cgtcggacca
     3961 ggccaggttg atttctcggt gaatcccgcg gattacggca tcccgccggg cgattacgac
     4021 gtctcggtgg tgaccaatac cggggaagaa gaggtgccga ttgaagtctc cggcgaagtg
     4081 gaagatgtgc gcatcccgct cgacggcagt tccccggtgc ttaacgttgc aggcgtgggc
     4141 gaagtgccgt tcaccatgat tagccagttt ggcgtacccg atgccgacag tgatgtggcg
     4201 taagaatcaa ttaacaggaa gacacaatga gttatgaaat tgccgcaacg gggctgaatg
     4261 ccgttaacga acagctggac gggatcagta acaacatcgc taacgccgga acggtgggct
     4321 ataagtcgat gaccacccag ttttccgcca tgtatgccgg aagccaggcg atgggcgtca
     4381 gcgtggcggg caccgcgcaa agtatttcgc gtggcggttc gctggtctcc accggcaatg
     4441 cgctcgattt agcgattaac gacgacggat tttttgtcat gtgtgacagc gcaggcaata
     4501 tctcctatac ccgcgccgga tcgtttgaaa ccgacaaaaa cggctatatc gtcaacgcct
     4561 cgggcgctta tttgcagggc tatccggtcg atgacactgg cactctgcaa accggtacgg
     4621 tcaccgatat ccagatcaaa accggcaata tcccggcgca ggccagcagc agcctgacct
     4681 ttaccgctaa cttcaacgcc agcgatgaag ctatcgatcg caccaccgtt ccgttcgacg
     4741 cgactaacag cagttcgtac accgacagct acaccaccac ggtgtatgac tcattaggca
     4801 acgaacattc ggtatgccag tacttcacca aaaccagcga caacacctgg gaagtgcagt
     4861 acaccttcga cggtcagcag cagaccggcg ttccggcgac gacgttaacc ttcgatccga
     4921 acaccgggaa gctgacctct ccaaccacgc cgcagaccat tgaatttcaa accgacgccg
     4981 ccgcgcccgt cgacttaacc gtcgattact ccacctgtac gcaatacggc tctgaatttt
     5041 cggtcaccac caacgccgcc aacggctacg cctccgccac gcaaaacggt gttcaggttg
     5101 atgacgatgg caaagtttac gccacctaca gcaacggcga gcgcatgttg caggggcagg
     5161 tggtgctggc gactttcccg aatgaaaacg gcctggaggc agtgagcggc accgcatggg
     5221 tacaaacggg cgaatccggc accccgctga ttggcgttcc cggctccggc acctgcggta
     5281 cgctgtcgtc gggcgtgctc gaaagctcta acgtcgatat caccagcgaa ctggtcaacc
     5341 tgatgaccgc ccagcgtaac tatcaggcca acaccaaagt tatcgccacc agcacgcagc
     5401 tcgatgacgc gctgttccag gcaatgtaat ggaccgcctg atttataccg ccctgagcgg
     5461 tgcttctcag accctgtatg agcagcaaat tagcgccaac aacctggcga acgtcaacac
     5521 caatggtttt cgcgccgata tggcaatggc aaccaacgac aaggtgaaag gcggcggctt
     5581 cgacacgcgc tatatggcgc aggaaggggc cagcggcgtg aacgacagca cgggtgttgc
     5641 ggagaaaacc gaacgcccgc tggacgtggc gattcagggc gcgggttaca ttgcggtgca
     5701 ggataaaaac ggtaacgaag tctatacccg caacggcaac attcagcagg acgaccaggg
     5761 gcaactgacc atcgacggca atctggtgct gggggataac gggccgatca ttctgccgcc
     5821 gaacgccatt gcttcgtttg gcagcgacgg cacgctgtcg gtgacgcccg atgacggcga
     5881 tgtgaccgcg acgatggata tcgatcgcct gaagctggtg gatatcccgg tggctaacct
     5941 ggagaaaaat gctgaaggga tgctggtgac tgccgacggc gttccggccc agcgcgatga
     6001 aaacatcaaa gtcagcggcg gttttctcga gggcagcaac gtctcggcgg taagcgaaat
     6061 gatgtcgtcc atcgccatga accgccagtt cgaagcacag atcaagatga tgaaaaccgc
     6121 cgaagatatc agcgacgcgg gcaaccgctt attgcgcggc tcgtaaccgc acatcgtagg
     6181 ccggataagg cgttcacgcc gcatccggca accgcggccc aaaaataaca cggaatttac
     6241 caccatgaac gcagcattat ggatcagcaa aaccggcctc tcggcgcagg atgctgaaat
     6301 gagcgcgatt gccaacaaca tcgccaacgt taacaccacc ggcttcaagc gtgaccgggt
     6361 gatgtttcag gatctgttct accagaccca ggaagcgccg ggcgcgatgc tcgaccagaa
     6421 caacatcatg ccgaccgggc tgcaattcgg tagcggcgtg cgcattgtcg gcacccagaa
     6481 aaccttcacc gaaggcaacg tcgaaaccac cgacaacgcg atgaatgtcg ccattatggg
     6541 gcagggattt ttgcaggtgc aaaaggccaa cggcgatatc gcctataccc gcgacggcaa
     6601 tttgcaggtg aacgccgatg gcgtactgac caactcacag ggcttgccat tgcagccgga
     6661 aatcgacgtt ccggcggggg cgacgaatgt tgcgtttggt gaggacggca cggtgacggc
     6721 gattcttcct ggcgacagcg acgtgaccga gttagggcaa ctgacgctgg ttaacttcgc
     6781 taacccggct gggctttccg ctgagggtga caacctgtat ctggaaaccg ccgccagcgg
     6841 tcagccgacc gaaggtgtgc cgggcgaaga tgggcttggc actttgcagg acaatgccct
     6901 cgaaggctcg aacgtagata tcgtcaacga aatggtggcg atgattacag tgcaacgggc
     6961 ttacgagatg aacgccaaaa tggtgtcggc tgcggacgat atgttgcagt acatcagtca
     7021 gacgctgtaa ggccgaaatg tcggatgcga cgctgacgcg tcttatccga cctacaaaag
     7081 cgcataactc actgtattta aacaggcaga acgtgaaaaa ttatctctgg ctggtggcgc
     7141 tgcttcccct gcttagcggg tgtgaatcgc aggccattct ggtcaaaaaa gacgacgcat
     7201 actttgcgcc accgaaaacc gaagcaccgc ctccggcaga cgggcgtgct ggcggtgtat
     7261 ttgaaactgg ctacaactgg tcgctgaccg ccgatcggcg ggcttatcgc gtgggcgata
     7321 tcctgacggt gatcctcgaa gaatccaccc agtcgagcaa acaggcgaaa accaacttcg
     7381 gcaaaagcaa caccgtcgat atcggcgcac cgaccatttt tagtcacact aaagataagc
     7441 tttccggctc tatcgacgcc aaccgcgact ttgacggcag cgccacttcc cagcagcaga
     7501 acagcctgcg cggggagatc accgtttccg tccatgcggt gcagcccaac ggcatcctgg
     7561 aaattcgcgg cgagaaatgg ctcaccctca accagggcga tgagtacatc cgcttaagcg
     7621 gcctggtgcg cgccgacgac atccagaacg acaactcggt ttcctcccag cggatcgccg
     7681 atgcctgcat ctcctatgcc ggacgcggcg cgttaagcga cgccaacgcc gcaggctggc
     7741 tgacgcggct gtttaaccat cctctgttcc cgatttaatg ccggagagtg caatgcaaaa
     7801 ctggataaag gccgcagtta tcgccgtgag cctggcgctg cctggtgtgg cgctggcgca
     7861 atcgctggag tcgctggtca acgtacaggg cgtgcgcgaa aaccaactgg tgggctatag
     7921 cctggtggtc ggcctcgacg gcaccggtga taaaaaccag gtcaaattca ccaaccagac
     7981 catcaccaat atgctgcgcc agtttggcgt gcaactgccg aataaaatcg acccgaaggt
     8041 gaaaaacgtc gccgcggtgg cggtgagcgc cacgctgccg ccgatgtact cgcgcgggca
     8101 gaccattgat gtcaccgttt cgtcgattgg cgatgccaaa agtattcgcg gcggcacttt
     8161 gctgctgacc caactgcacg gcgctgatgg ggaagtttac gccctggcac agggcagcgt
     8221 ggtggtgggc gggatgaacg ccaccggggc cagcggttcc agcgtaacgg ttaatacgcc
     8281 aaccgccggg ttaattccta acggcgcaac ggtggagcgc gagatcccca gcgattttca
     8341 gatgggcgat accatcaccc tcaacctgaa acgcccgtcg tttaaagacg ccaacaacat
     8401 tgcggcggcg atcaacgcct cgtttggcgg tattgccacc gcgcagagtt ccaccaacgt
     8461 taccgtgcgc gcgcccacca gccccggcgc gcgggtggca tttatgtcgc aactggacga
     8521 tgtgcaggtg caggcggaga aaatccgcgc tcgcgtggtg tttaactcgc gtaccggcac
     8581 ggtggtaatg ggcgatggcg ttgcgctaca cgcggcggcg gtgtcgcacg gcagcctgac
     8641 cgtttcgatt aacgaaacca gcaacgtcag ccagccgaat gcctttgcgg gtgggcgcac
     8701 ggcggttacg ccgcagagca atatcgcggt gaatcatgcc cgtccgggcg tggtgagttt
     8761 gccggagtcg agcagcctga aaacgctcgt caatgcgctg aacagtttag gcgcgacgcc
     8821 ggacgacatt atgtcgattt tgcaggcgct gcatgaggcg ggcgcgctgg atgccgacct
     8881 ggaggtgatc tgatgaaagt gaacgccagc ggcgggattg acggcagcga cgcgttaatg
     8941 gggccgaagg ttcaggccaa tgatatcaag caggccgccg aacagtttga ggcgattttt
     9001 ttacgcaaca tgctgaaaga gatgcggaaa accaacgagt tgtttgattc aaaagataac
     9061 ccgtttaaca gcgattcggt gcgcatgatg caggggtttt atgacgatga gttgtgtaac
     9121 accctggcgc agcagcacgg gattggcatt gcggcgatga ttgtgaagca gttgtcgccg
     9181 aagcataaat gagcttcggc gttcggtgca cgatgcgacg ctgtcgcgtc ttatcaggcc
     9241 tacgagtcgc ccgtatgtag gccggataag gcgtccacgc cgcatccggc gttcggcacc
     9301 cggtttacgg aacttaagaa tccccaactt tacgccgctt ttaaccggca cgatttaccg
     9361 gtaaggatcc aacatggaca tgattaacat cggctacagc ggtgcatcaa ccgcgcaggt
     9421 ggagcttaac gtcacggcgc aaaacaccgc caacgccatg accacgggct acacccgtca
     9481 ggtggcggag atcagcacca tcggtgccag cggcggttcg ccgaacagcg ccggtaacgg
     9541 cgtacaggtc gacagcattc gccgcgtctc caaccagtat caggtgaatc aggtgtggta
     9601 tgccgccagc gattacggct attacagcac ccagcagggg gatctcagcc aactggaagc
     9661 ggtactgagc gacgataaca gcagcctgag cggcggcttc gataacttct tcgccgccct
     9721 taacgaagca accaccagcc ccgatgattc cgccctgcgc gagcaggtga tcagtgaagc
     9781 cggggcgctg tcgttgcgta ttgataacac gctggattac atcgactcgc aaagcacgga
     9841 aatcatcagc cagcagcagg cgatggtgtc gcaaatcaat acgctcacca gcggcatcgc
     9901 cagctataac cagcaaatcg ctcaggctga agccaatggc gataacgcct ccgcgctgta
     9961 cgacgcccgc gaccagatgg tggaagaact gagcgggatg atggatgtgc aggtcaatat
    10021 cgacgaccag ggcaactaca acgttaccct gaaaaacggt cagccgctgg tgagtgggca
    10081 gcaaagttcg accatcgcgc tggaaaccaa cgccgatggc acgtcgacca tgtcgctgac
    10141 cttcgctggc accacctcga cgatgaccac cgacaccggc ggttcattag gcgcactgtt
    10201 tgattatcaa aacgatgtgc tgacgccgct gaccgacacc atcaacagca tggcgtcgca
    10261 gttcgccgat gcggtcaaca accagctggc gcagggctac gatctcaacg gtaaccccgg
    10321 cgagccgctg tttatttatg acgccagcaa tgccgatggc ccgctgaccg ttaacccgaa
    10381 tatcaccgcc gatgagctgg cgttttccag ttcaccggac gaaagcggca acagcgacaa
    10441 cctgcaggcg ctgatcaata tctccaccga accgctggaa atagccaacc tcggcagcgt
    10501 gacggtcggg caggcgtgct cgtcaatcat cagcaatatc ggcatttaca gtcagcaaaa
    10561 ccagacggaa gtcgatgccg cgtccaatgt ctattccgca gcgcaaaacc agcagagcag
    10621 cgtcagcggc atcagcatgg aggaagaggc ggtgaacctg atcacctatc aacaaattta
    10681 tgaagccaat ctgaaagtca tttccgccgg ggccgagatt ttcgattcgg tgctggaaat
    10741 gtgcagctaa gcgggagcgc gaaaatgcga gtaactaccc aacaaaccta cgtctcgatg
    10801 acgcaaagtt ttaataatct ctctggcgat ctggcgcatg ttgtcgagca gatggcaacc
    10861 ggcaagcaga ttttgcagcc gtcggatgac ccgatcgccg ccacgcgaat cacccagtta
    10921 aaccgccagc agtcggcgat tgagcagtat cagagcaata tcgactccgc gtcggcgggc
    10981 ttaagccagc aggagtcgat ccttgatggc gtcaacaaca gcctgctggc ggtgcgcgac
    11041 gacctgctgg aagcggcgaa tggcaccaat accgccgatt cactcgcaag cctcgggcag
    11101 gatattgagt cgctcaccga atcaatggtg gcggcgctca attatcagga cgaagacggg
    11161 cactacgtgt ttggcggcac catcaacgat cagccgccga ttgtggcagt ggacgatgac
    11221 ggcgatggcg tcaccgacag ctacagctat cagggtaaca gcgaccaccg ccagaccacg
    11281 gtgtcgaacg gcgtggaagt ggacaccaac gtggcggcga gtgatttctt tggcagcaat
    11341 ttagacgtgc tcaacaccct gaattcccta tcccaggagt tgcaaaaccc ggacgtcgac
    11401 ccggcagatc cgcaggtgca gagcgatatt cagaatgccg ttgatgtggt ggacaccgcc
    11461 tcggacgacc tcaacgcctc gattgcgtca ctcggcgaga cgcaaaatac catgtcgatg
    11521 ctgagcgatg cgcagaccga tatctccacc tctaatgatg agctgattgg ttcattacag
    11581 gatctcgatt acggcccggc gtcgatcacc tttaccggac tggaagtggc aatggaagtt
    11641 accctcaaga cttactcgaa agtgagtgag ttgaatttgt tcagcgttct ctaactcttt
    11701 caggaacagt atgcaggtcg gacttacatc ttcatcactc gccaccggcg gcgcgcatag
    11761 cgctgcggta tcgtcgtcaa ccgttgcgcc cactcaggcg gtgcggcaaa aactcccggc
    11821 gacggcgagt gaatatccgg cctcgccgct gattaccact cgcccgcagc gttacagcgt
    11881 gcagcttaac gaccagctca ccacgttgca gcaggcggat cattatctcg ggcaactgga
    11941 gcagcagttg ctcgactatc gtcacagcca gcgtaaaggc gggcaggcgc agaatacggc
    12001 gctgatgcag atgctggaca aacgcaccgc gctttccggc ggtgcggtgg accgccagtt
    12061 gcagccggtg ttacagggcg aagcgcgggt gacctttcac tcgccggatc tggcgaatct
    12121 ggtacacaat cccacgccgg gaacgcgcat gttcagcgtc tctgacgggc ggcagactca
    12181 gctttcggcg gtgatgctgt ctgaggatga cagcgccgcg cagtatcaga cgcggctgac
    12241 caacgccctg cgccgcgtgg gcgtgcagct acatcagcaa gcggacggca tcagtttctc
    12301 caccaccgaa aaacagtggc cccatatcga acgtacctta agcgtgcgca ccgatggcga
    12361 taagtcggcg tttatgccgc tgaaaacttt tgccgagccg tctgaggcag accgtttagc
    12421 tcaaagcctg caacagggcg gtgcgggaat ttctcagacg ttagaaagca ttaatcagca
    12481 gcgtgcgcag atggcggtac agcaggaaaa ggcgcggcag ttgatcgacg gcatgtcgcg
    12541 cttcccgcaa acggaaaacg cggtgcaggc gtcggaaaac ctcggcggcg tgctggatag
    12601 cgcgaatcat aactatcagg ttttattgca ggcggtgaat ggtcaggcgc gaatttccag
    12661 ccagacggtg aggaatttat tggggtaatt atcagccgga taagacgcgt ttagcgtcgc
    12721 atccggcata tattggcact catcagcatt tctgcaaagt atttaacaac ttctctaaat
    12781 tctgatacat cgcgctatta atatcgctgt tctgccagcc ttccacatta aaagttaatt
    12841 gccagctacc attgcactgg ttgcgcagaa caatatcact ttccagtcgg ttacgaacat
    12901 atttataata ggttgtcatt gtcatattat gcgctgacaa taaattatcg agtttgtctt
    12961 ttcctggtgc gagggtttgt gacagtaacg gtacggaatt attttctttt ccagaactaa
    13021 ggtgaaatat ttttattctt ggactgttaa ttgactcttt taccagttgc ggggcatcag
    13081 gtgtactttt caagtgtgaa taccccacgg tcgagccaat aaatataaca gccgcggcta
    13141 acgctagccc cattattact ttatgttttt ttctaacagg cacaggcggt gttgtagaag
    13201 atatctcttc tggtactgtt tcctgtatct ggtcggtaat aatcagtttt tttgcaggcg
    13261 atgagtccgg caaagacaca tactctttat tgcataagta acctttcttc ggaattgttt
    13321 taataatgtt ctgttgtttg ccatcatcat cgatagcaac gcgcaacgca tggatcgccg
    13381 tcggcaggct gtttccgccg acaatgcggt tcttccatac ttcggcacat aaataagatc
    13441 gcgataaaac cacatcggca ttttttgcta atttttccag caaaataaaa tggtattcgc
    13501 cgagacgacg tgtttcaccc gtttcgcaat gtattaaggc attaagcgac gggtccatcc
    13561 gccagttgtt gataataata ctcatagttg agtttacctt agcaagggat aaatgtttcc
    13621 tgatagtgct ttgcataaag cacagtctta tacgtccctg agaagatgat ttttctgcgt
    13681 gataactaat attttatggt aataggatta ttcttaatca ctatatggca tggaaatgta
    13741 acgaaatgac aggactggta caatagccaa atgtgtacta tttgcaacgc aatgtaaatt
    13801 ttggcatcag tacattgaga agaaaacgcg ttaattgatg gtaatcttaa ctatatcaag
    13861 cgtttgataa agacatattt tgatacctgg ccatgtggca gatattgcta ataatctttt
    13921 tatctgaatt taaactctgc tttttttagc taaaagtggc atcagataaa tcgcagtgaa
    13981 atttttttaa cggcggtttt aagtttgctc cttaggtgtc gtttttacag ggtatcgaaa
    14041 cgaaaacgat taatcccaag gagcagtatc atgttatcta ttaacaccaa taacgcctca
    14101 atggccgccg ttaatgcaat cagcaaaagc agctcttctt tgtctacatc tatggaacgc
    14161 ctggcgacag gtaaccggat taactcttct gccgatgacg ccgcaggcaa acaaattgct
    14221 aaccgtctga cagcacagtc cagcggtatg ggcgtggcgc tgagcaacat caatgacgca
    14281 accgcgatgc tgcaaaccgc cgatagtatg ttcgatgaaa tgtctgatgt gctgggtcgt
    14341 atgaaagacc tgtcaactca ggcagcgaac ggcacctaca gcgacgacga tctgcaggcg
    14401 atgcaggacg aatacgacga actgggtcag cagatgtctg acatgctgca aaacaccact
    14461 tacggcggca ccaacctgtt cggtgtttcc ggtaccagca acaccggtac tgacggtctg
    14521 ttccagagcg cggtaacttt ccaggtgggc gcggaatctt ccgacaccat gaccgtgaac
    14581 atctccagcc aactgaacac gctggtgacc gatctttctg ctatcagcaa ctcctttagc
    14641 gcggatcagg cagacaccac gggtactgca ggtgtttctg gcggtactga gctgaccgcg
    14701 tctggctcgg ctaaccagat gatcaccagc atctctaccg cgatggatga cgtttcgcag
    14761 atccagtcca agctgggtgc gtctatcaac cgtctgaacg acaccgccaa caacctgacc
    14821 agtatgcagg acaacaccga agtcgctatc ggtaacatta tggataccga ctacgcgacg
    14881 gaagcgtcca atatgaccaa acagcaggtg ctgatgcaga ctggcatcac catgctgaaa
    14941 cagtccaaca gcatgtccag catggtttcc agcctgctgc agtaatggcg gacttgccgg
    15001 atgcggcgta aacgccttat tcggactacg aacaacaccg cctgcgggcg gtgttgtgct
    15061 ttctgcggca ggcttttttc cgccagaccg gaaacgccat ttccgcctgt tttttaatct
    15121 attgattatt cacataaaaa atattggcac gccttttgcc aataacctga catcgttatc
    15181 cggagtcatc aatgatcaac ccaagaacca ttgcgcaaga aatcgcgtat gcggatgtcg
    15241 ccactcaggc ggcaaacttg caggagaagc aaacagagct ggatgctgaa agcagcggcc
    15301 tggattcgct cagttcagcg ttgagcgatt ttcagagcgc cgttgacgcg ctgaacagcg
    15361 ataccgacgg cccggtgacg tttgccgcca ccagcaataa tgactcggcg agcgtttccg
    15421 ccaattccca ggcgcaggcg ggaagctact catttttcgt tgagcaactg gcacaggggc
    15481 agcaaaccac gttcagtatg ggcgacgacg ccttttccgc caccggcact tttgaactga
    15541 cgatgggcga cagcaccatg gatatcgatc tgtcggcggc agatcagaat ggtgacggcg
    15601 acggttttat cgacgccagt gaactggtga atgcgattaa cgactccgat gataatccgg
    15661 gcgtgtcggc ggcgctggta aaaaccgacg gcaccacaac gattatgctc acctcggata
    15721 gcaccggggc gcagagcgcg ttttcggtga gcgtaacggg gcatgatgcc agcaacgata
    15781 gcaccagcgc gcccgttgcc acggatgttt cctccgctca ggacgcgatt attcatcttg
    15841 gcagcgccac ggggcctgag atcaccaaca gcagcaatac gtttgatgat gtgattcccg
    15901 gtgtcaccat gactttcacc gaagtcagcg attctgacag cgatctcact acctttaaca
    15961 tcagcgaaga ttccagcgcc agccaggaga aagtacagac ctttgtcgat gcctataaca
    16021 ccttgattga tacggttgat tcgctaacca cccacggtga tgacagtacc agcgccgggg
    16081 tatttgcagg cgacgccgga ctcagttcac tggcgaacca gctcgatgac atcgcccatg
    16141 ccagctacaa cggcgtgtcg attgttgact atggcatcac cctcgattct cacggccact
    16201 tacagattga ctccgaccag ttcaacgacg aaatggcgaa aaatcctgac ggtctgacct
    16261 ctattttcgt tggcgacaac agcatggtgg cgcagatgga cgaccttatt aacacctaca
    16321 cggactccag caacggcatc atcaccctgc gccagcagaa cattgacgat cagatgagca
    16381 aaattcagga cgaaggcgat cagcttactg atacctataa cgccaactac gaccgttatc
    16441 tcgaggagta caccaacacg ctggtcgagg tgtacaccat gaaagccagc atggcggcat
    16501 tcgcgtaact cagaaggaat atccctaaag atgtaccaag tacaggacgg ctattcgcaa
    16561 tataaagaga tcgatctcgc ggcgcgtact gcggcggcat cgcctctgga actggtgctg
    16621 gtgctgtttt ctggcctgat ggacgaactg gaacgcgcca aaagccatat tgaagggcgg
    16681 cgtttcgaga agaaagccca gagcatcaat aaatgcatcg acattctcaa tgcgctcacc
    16741 agttcgctgg agtttgaaac tggcggcgag ctggtggtca acctttcgcg actctatgac
    16801 cactgcgttt accgattgta tgaggccagc ggcgaactgt cggcagagaa aatcgatgaa
    16861 gtgatgctga tcttaagcaa cctgcgcgaa ggctgggaag ggctttcggg caagctgggt
    16921 taagacgatg gaaaaacaac gcaggaaact tttcgcactg gtcgaggcga tgaatgaagc
    16981 cctcgacaag caacgctggc gacgccttcc ggcactgcat cagcagttga tacgccagtt
    17041 tcacgattac gcggcggcgg aaaccgatgc ggcgcagttg gatgcagtga aagcgcgact
    17101 gtatgacgcg tttaacgccc tgattaaacg gcgcgagcag cgcgcagaag tgttgaaagc
    17161 gcgtatggaa cagcatcagc ggcatcagga aggcgtactg gcctactcaa ttgttaattt
    17221 actttcggag aagtcatgaa tccgacattg cttgccacgc tcggaacgct tgcggaaacc
    17281 gcttcgttga aggcggatat tttaccgccg gtgagcggtg aaaacgcgcc cgcgtttacc
    17341 ctgccgaaaa tggcggtcgc ggcggtggcg gagcgcgttc atagcgcaaa aaccagtcag
    17401 caacaggcga cgcgcccgca ggagaacgat ccggtggcga tgcaggcgct aatggcgctg
    17461 ttacttccgc aacctgccgc gccgcatcag gacacgccgc agccgcgaaa cgtcgcaaca
    17521 tcgccggtta tccagcaatt gacgaaagcg gtggtgcaaa acgcgccgca acgccagacg
    17581 caacagcagg aactcacgcc gttgccgccg cagttgcagg aactgatcag tcagttgccg
    17641 caggcgaagc ccgaacagca ggccagactg gcgacttacg ctagtgaaga tttacatgcc
    17701 attacgccga cgcagccgcg cgtctcaaca cagccagctc gcccgaaatc tgaactaacc
    17761 cgtgtgaccg cgcgcccgca ggtcgagcgt aaaacggaaa aagtgccgga cagtgaaccg
    17821 gttattgcgc gtgcggtgtt gcaggttaag acgccggagc tgctcagcga tcatcaggag
    17881 attgtcgcca aaccagccac gctttcgatg gacgaactgg gcgaaaaact gacgacgctt
    17941 ttgaaagatc agatccactt tcagctcaac aaacaacagc agatctccac catccgtctc
    18001 gatccaccgt cgcttggcaa gctcgagatc gccgtacaac tcgacaacgg caaactgatg
    18061 gtgcacatcg gcgcgaacca aagtgaagtt tgccgcgcgt tacagcagtt tagcgacgat
    18121 ctccgccagc atctgacggc gcaaaatttt atggaggtga acgtacaggt ttcctccgaa
    18181 gggcagtcgc agcaacaaca acagtcgggc catcagcagg aagaggtgag tgccgcctta
    18241 cagcttgatg atgcgcctca atttcaacag aacgaatccg ttttgatcaa agtgtaaagg
    18301 caatatgaag aaaatcgtgg tggcgggcgt ggtcagcgcc gttctggcgt tggtggtggg
    18361 ggccggagct ggatggggtg tctggcatca ttatgccgga aaaggaaaac cgactgccgc
    18421 cgcgcagacg gaaacggtgg aaaccctgga tgaaagcaaa agcgtgtttg tgacgttgcc
    18481 ggaaaccatt gtcacgctgc atgacaacaa cggcgcggac cattatttgt cggcggaatt
    18541 agtgatggtg gtcgccagtg acaaagaagc ggaaaaaata aaacatcagg agccgctgta
    18601 tcaaagtatt gcggttgaat gcctgacgga aatgaaattt gaagatttgc gcggcatgaa
    18661 aatatctgcc atccgcaaac tcatttctga cgcgttaaag aaagatcttc agcgtcgcaa
    18721 aatgagcgca ccgtataagg atttgctggt gaaaaaagtg gtcttccagt aatcaccagg
    18781 cggatataaa catgctacag gatgtattcg agtcagaaga atttgctgcc gcgccggtat
    18841 taaccccgca gcaggagagc cactacttgc aggcctattt accgttggtg cgtaaagtgg
    18901 tgcggcaact tgccccgcag tgtaattgca ttattgatcg tcaggatatg gaacaaacgg
    18961 cgttaatggg gctgcttaat gccatccgcc gctacgggct gccggacgaa ggttttgcgg
    19021 gctatgccgt gcaccgtatt cgcggcgcga tcctggatga gctgcgcgct ctcgactggc
    19081 gaccgcgcca gctgcgacag aaatatcatc agatgaaaga tctgattcgc gaaacgcgca
    19141 aaaaactggg gcacgaaccg gagtggtcag aactggccgt ggaagggatc tcacatgaag
    19201 attatctcga atatcagcaa ctggaagggg cggaaacgct cgccagcctg gacgaactgc
    19261 tgaacggcga agggccaggc attccgcttg ttggccggga gctggaagat cagtttgtca
    19321 ctcagcaaat gctgcaacag gcgctttcac aattaagcga aaaagagcaa ctgatcctct
    19381 ccatgtatta ccagcatgag atgaatctca aagagattgc gctggtatta gggctaaccg
    19441 aggcccgaat ttgtcaactg aataaacaga tcgcgcagaa aattcgtgat tttgtctatc
    19501 cgaattaaag tgaagaaatc atgcaaaaaa tattaggttt actggtggtg ctcggctgcg
    19561 tatttggcgg ttattttgaa gcgggcgggc aattagtggc tatctggcaa ccggcggaga
    19621 tgatcattat tttgggtgcc ggtttcgggg cgatgattat tggtaacccg aaacatgtgc
    19681 tgaaagagat tgcccatcaa attaaaggtg tgatcagtaa aaaacagctc gggccggagt
    19741 ttcagcgtca gctattaatg tgcctgtatg agttgctcga aatggtgcaa aacggtggtc
    19801 tgcgcatgct ggatcaacat attgagcaac cggaagagag caccattttc cagaaatacc
    19861 cgctggtgtt aacccagaag cggctggtga cctttattgc cgataacttt cgcctgatgg
    19921 cgatgggtaa aatcgacgcc catgaactgg aaggcattct cgatcaggaa ctggacactg
    19981 cggaagagtc gctgctcacg ccgtcgcgct cgctgcaacg taccgccgaa gcgatgccgg
    20041 gatttggtat ttgtgcagca gtgctgggga tcatcatcac catgcagtcg attgacggct
    20101 ctattgccct gatcggcctg aaagtggcgg cagcgctggt ggggacgttt ttaggcgtct
    20161 ttatctgcta ctgcctgatg gacccgctcg ccaacgccat ggaacaacag gcgcgggcag
    20221 aacattcttt gctggaatgc gtgcgcactg tgctggtggc gcaggcaggc ggcaaaccga
    20281 cgctgctggc ggtggatgcc gggcgtaagc tgctgcatct ggcgtccaaa ccgaccttcg
    20341 ccaacctcga cgcgtgggtg aatgcgatgc tggagcaaga ataagcatga gaaagacggg
    20401 caaccgccgc gagcgtgggg caaaaaccac catcgtccgg cggcagatca aaaagaatca
    20461 tgccgggcat catggcgggg cgtggaaggt ggcgttcgcc gactttaccc tggcgatgat
    20521 ggcgctgttt atgacgctgt ggattgtcaa cagcgtcagt aaatccgagc gtgaaagcat
    20581 tatcgccgcg ctgcacgggc agtcgatttt taacggtggc gggctgtcgc cgttaaataa
    20641 aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag agacggagaa
    20701 aaaagcgcgt gatgtgaatg agaaaacggc gctgctgaag aagaaatccg ccaccgagct
    20761 tggcgagctg gcaaccagta tcaacaccat tgcccgtgat gcgcatatgg aagcgaatct
    20821 ggagatggag attgttcccc agggattacg cgtactgatt aaagacgacc agaatcgcaa
    20881 tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaacgctgc tggtggagct
    20941 cgcgccagtt ttcgactcgc tcgataacaa aattattatt accgggcata ccgatgcgat
    21001 ggcttacaaa aacaatatct acaacaactg gaacctttcg ggtgaccgcg cgctttcggc
    21061 tcgtcgggtg ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg taagcgcaat
    21121 ggcggaccag atgctgctgg attccaaaaa tccgcaaagc gcgggcaacc ggcgcattga
    21181 gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg
    21241 ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg
    21301 aacgcgttaa atgctgaatc tttacgcatt tctcaaaccc tgaaatcact gtatacttta
    21361 ccagtgttga gaggtgagta atgcgtaaaa tcattcatgt ggatatggac tgctttttcg
    21421 cagcggtgga gatgcgcgac aatcccgccc tgcgcgatat ccctattgct attggcggca
    21481 gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac
    21541 gtagcgctat gccgacaggg atggcgctca aattatgccc gcatctcacc ttgcttccgg
    21601 ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca
    21661 cctcgcgtat tgaaccgttg tcactggatg aggcttatct cgacgtcacc gatagcgtcc
    21721 attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt ttcagcgagc
    21781 tgcaactgac agcgtctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg
    21841 acatgaataa acccaacggt cagtttgtga ttacgccggc agaagttccg gcatttttac
    21901 aaaccttacc gctggcaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag
    21961 cgatggggct gcggacctgc ggtgatgtac aaaagtgtga tctggtgatg ctgcttaaac
    22021 gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg
    22081 ttaacagcga acggttacga aaatccgtcg gcgtggaacg cacgatggcg gaagatattc
    22141 atcactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc
    22201 tggcaaaggt aaaacctgat ttactgatcg cccgtcaggg agtgaaatta aagtttgatg
    22261 attttcagca aaccactcag gagcacgtct ggccgcggct gaataaagct gacttaatcg
    22321 ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc
    22381 tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgaataa
    22441 catacaaata agagactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
    22501 agcggttacg atgactgcca gtcagcatta ttcaccacaa caaattgccg cctgggcgca
    22561 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
    22621 taatgcaaaa ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt
    22681 tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt tgattaagtc
    22741 tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg
    22801 ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta ttaattttta
    22861 tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc aagcctagta
    22921 tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
    22981 atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc
    23041 catttcctga ttttttaatt gttactctca ataattcaag tcgcaacaca tgcaacttga
    23101 agtatgacga gtctatttac ggaatggtat atgggcaatt atattcgtcc cttatccgat
    23161 gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta
    23221 cacaccacgg caaatttacc caacatgcag cgcgtagtgg ggatgccaga tttacatccc
    23281 ggacgcggct atccgattgg cgcagcgttc ttctctgttg gtcgttttta tccggcactg
    23341 gtcggcaatg atatcggctg cggtatggcg ctatggcaaa cagatattct cgctcgcaaa
    23401 tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc tgaagaaagc
    23461 tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc
    23521 tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct
    23581 gaactgtttg cactggcagg tctggatgca caacatctgc aactgttggt tcatagcggc
    23641 tcgcgggggg tggggcagtc tattttacag cggcatattg cctcgttctc gcatcatggt
    23701 ttgcctcaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga tgcgctggcg
    23761 tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt taaggccacg
    23821 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa
    23881 cagggctggt tgcatcgtaa aggtgccacg ccggatgaca acggtctggt gattattccc
    23941 ggttcacgtg gtgattactc ctggattgta cagcctgttg cgaacgaaaa aacgctccac
    24001 tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
    24061 gcgaaataca cggcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc
    24121 gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg
    24181 caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
    24241 ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctca gcgcaggggc
    24301 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
    24361 gacaagacgt cgcggtaacg gtgctggaaa cagaaacggg ccactactct gacacgctgc
    24421 gttcggcgct gatttctctg gatggcgata acgcatgggc attaagcgaa agctggtgtg
    24481 gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt
    24541 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
    24601 agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
    24661 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg
    24721 ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa
    24781 atagcgcggt gctgaaatcg ctgcggcgaa tgttccatca ccagattgaa cgtggcaacc
    24841 cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
    24901 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
    24961 gcagttcagt cagcagcgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
    25021 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
    25081 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggt gttttgttga
    25141 acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
    25201 agccaggata tgcgcctttc gcttcggttt tcgcgccagc tagtttaccc agcgaatcca
    25261 gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt
    25321 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
    25381 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
    25441 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
    25501 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
    25561 gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
    25621 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg
    25681 catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt
    25741 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
    25801 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
    25861 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
    25921 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
    25981 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac
    26041 caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
    26101 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
    26161 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
    26221 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
    26281 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
    26341 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
    26401 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
    26461 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    26521 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    26581 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    26641 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    26701 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    26761 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    26821 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
    26881 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    26941 actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
    27001 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
    27061 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    27121 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc
    27181 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    27241 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    27301 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    27361 ccgtctgatg tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg
    27421 tattgtgatg agcgatgctg aacgtaccga cgatgattta tacgatactg tgattggcta
    27481 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
    27541 ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
    27601 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
    27661 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
    27721 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
    27781 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
    27841 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccatc
    27901 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
    27961 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
    28021 ccgtttcggc gctaacgttg ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc
    28081 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
    28141 ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga
    28201 tgacgcgtta cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg
    28261 tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga
    28321 ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa
    28381 cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg
    28441 cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga
    28501 gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc
    28561 actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct
    28621 ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gagttctggg gctggtggat
    28681 ggagcttgaa gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa
    28741 agcaggcgac tggaagagtg ttccggtaaa agacactgaa gtggttgaac gactggagca
    28801 caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct
    28861 ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc
    28921 cggatgcatc acatccggca ataatcatta aaactgatag gtcatgccaa ccgcgacaat
    28981 atcatcatta ttaatattca atttgttatc gctatccagt tggttgattt tataatcaac
    29041 aaacgctgac atatttttgt tgaaataata tgtagccccg acatcgatat aattgaccag
    29101 atcttcatca ccgatacctt caatatcttt ccctttcgat aagacataac ccagcgatgg
    29161 acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg tcttattggc
    29221 aaagccgcca gttattggcg tcattttgcg tgtttcagaa taaaaagttg ccagataaaa
    29281 attattggca tcgtatttca gacctgtagc ccatgcttct gcacgcttgc ctgtgccacg
    29341 gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggccccac taatggcgaa
    29401 atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt
    29461 aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc catcgataac
    29521 gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt
    29581 gtcggtctgc gccgaggaat cgccaccaaa ttccgggaac atatcggtcc aggcttccac
    29641 gtcatacaac gcgcccaggt tacgaccata atcgaaagaa cccaaatctt tatatttcaa
    29701 cccggcaaaa gcgagacgcg ttttttgctg tgcggtatca ctctccgctt tatttccggc
    29761 aaactccgct tcccaccgac cataaccagt cagttgatcg ttaatttgtg tttcaccttt
    29821 aaaaccaaaa cggatataac tctggtcgcc atctttactg gcgttatcac tcatataatg
    29881 catggcttta actttgccat agacatccag tttattaccg tctttattat atatttctgc
    29941 agcctgtaca gatgcagatg ccacaatgcc catcaccact aatgccagag tgctcttttt
    30001 cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc ccgttgaaaa
    30061 caggaaagtt tttaacctga gattgttaaa gatatattac agat
//
LOCUS       DAZFDP010000651        14693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.125, whole genome shotgun sequence.
ACCESSION   DAZFDP010000651 DAZFDP010000000
VERSION     DAZFDP010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.125"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..238
                     /locus_tag="ACHJ1G_005967"
     CDS             <1..238
                     /locus_tag="ACHJ1G_005967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767547.1"
                     /translation="LGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQ
                     KSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHJ1G_005968"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHJ1G_005968"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4767548.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHJ1G_005969"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHJ1G_005969"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4767549.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHJ1G_005970"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHJ1G_005970"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4767550.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHJ1G_005971"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHJ1G_005971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4767551.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6382..6696)
                     /locus_tag="ACHJ1G_005972"
     CDS             complement(6382..6696)
                     /locus_tag="ACHJ1G_005972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO4767552.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6693..7007)
                     /gene="yjeN"
                     /locus_tag="ACHJ1G_005973"
     CDS             complement(6693..7007)
                     /gene="yjeN"
                     /locus_tag="ACHJ1G_005973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO4767553.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="ACHJ1G_005974"
     CDS             complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="ACHJ1G_005974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4767554.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="ACHJ1G_005975"
     CDS             complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="ACHJ1G_005975"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4767555.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10082..11890
                     /gene="frdA"
                     /locus_tag="ACHJ1G_005976"
     CDS             10082..11890
                     /gene="frdA"
                     /locus_tag="ACHJ1G_005976"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4767556.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11883..12617
                     /gene="frdB"
                     /locus_tag="ACHJ1G_005977"
     CDS             11883..12617
                     /gene="frdB"
                     /locus_tag="ACHJ1G_005977"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4767557.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12628..13023
                     /gene="frdC"
                     /locus_tag="ACHJ1G_005978"
     CDS             12628..13023
                     /gene="frdC"
                     /locus_tag="ACHJ1G_005978"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4767558.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13034..13393
                     /gene="frdD"
                     /locus_tag="ACHJ1G_005979"
     CDS             13034..13393
                     /gene="frdD"
                     /locus_tag="ACHJ1G_005979"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4767559.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            13456..14589
                     /locus_tag="ACHJ1G_005980"
     CDS             13456..14589
                     /locus_tag="ACHJ1G_005980"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4767560.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT         3643 a         3920 c         3803 g         3327 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggccgcttta aactcggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgc caggccacca
     3901 gccagacgtt acaggttcgg caggcgctga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggaact gaccaagctg aaagtctcca
     4261 acgggcaact tgaggatgcg ctgaaagagg tgaatgaagc gacgcatcgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccact ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtacctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccactgg ttgccattct ggcgtcagcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggag attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taagtttgtg
     6361 tgccggatga accaatccgg ctcacaaaca aaactgatag cgcagaaaaa actgccacag
     6421 ccagtagccg aaaatagcca gcgcggtggc gtacataaac cagccgcgtt tttttcgcca
     6481 caaagcgaac cagagaaaag ggaaaaagag cggtaacgtc attatcacac caacatcacg
     6541 ggtatcgtct tcttcgagat acatcaacac atcgcagacg ttgttaatcc cgcgtccatc
     6601 aatcatccac tctttgtcca gcgcggaagt gatggttatc cagaggaacg ccacaataaa
     6661 ccagatgcag cataagacaa acatgcgcgc actcataagg taaccggtcg cttaatttcg
     6721 ctatagcgca tacgaaacaa atcgccgtat cccactaatt ccccgcgaaa aaccagacat
     6781 cggataagat aagcgcaata aatatccggc aggtttcccc accgtttacc atcgcgatct
     6841 ttgagggtgt taatggtgct acccaccacc atcgccactt tacgggtgtg gcaggcatcg
     6901 acaaaagaga gaatcgtctg ctctatttcc gctacatccc cggcagaaaa ttgcagcgcc
     6961 ctgatttcat cttcggttat tgccgggtcc cggctggtgt catccataac atttcctgtc
     7021 tttacgccct atcgtcaggt agacgatagg gcatcgggtt attccaccgc ccactgcgga
     7081 ttttttgcca ctctgctgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
     7141 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggttga
     7201 atgatggtga agacgttcgc aaatgtcacc accagtacga caaccactgt tgcaatcatt
     7261 gccgacatat gcgttttgaa aatcacaaat ggtctgtcga gatcctgacg tgctttaaaa
     7321 aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca tagacacgtt cgccatcagc
     7381 gtcagcttgt taaagaacgc cgatgcggta ccgccaccaa acgaaaccag caggatgaag
     7441 acagtaacca acccgcactg catccacatg gcgatagacg gcatccccat cgcattcagg
     7501 cgcgtcatcg gttccggcca caacgctttc ggcgtcccct ggatgatggc tttcagcggt
     7561 gaatagcaaa gcgtaaagaa cgcaccggta taagcgagga acatcgaaag tccggtaata
     7621 cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg acaaatgcag tgcgttaccc
     7681 agcgtcaccc cgaggctctt catcagcaca taggtaatat tgccaaggtt aacggaacca
     7741 ttacttaata cctgctgcca gtttgtgctg acgccccata aaaatattgc cagcgaataa
     7801 ccgattgaaa taacaatagc ggcaaaaaca atacctttgg caaagttctt ttctggattt
     7861 tccgttttat cgaccagacc accaaccgct tcaattccgc cataggcaaa aatagcaaat
     7921 accacaaacg atagcattgc cagaccggac tgataacctg ggttcggtga tgcaaggaaa
     7981 ttaatatcct gcgcgaaatg cccaccattt aataacaaaa tagtaatgct tactaacagc
     8041 aatactaaat tcagacacat tactgcaata ccgcccaccg cagtaatgcg ggcaatttta
     8101 ttaatcccct ttgaagcaac gacggtgacc agaatcatcc atgccactgc cagcagacca
     8161 accacctgcg taggctccag tccagcaata cgccagtgct gggtcatgtc gctaccatag
     8221 aggaatgttg agaacggtac ccaaactttc gcggaggtac tcaccatcca gatgatataa
     8281 gaggaaaacc acataaacgt accaatgaag gcaaaacgtg gtccgacact attattcatc
     8341 caggaataga taccgccttc ttctttgcga taagcagctc ccatttcagc catcattaag
     8401 gcgaatggaa taaagaataa caatgcagaa aatatataaa agggaatcgc actataaccc
     8461 attaagtaat aagccgatgg gctattggca aatccaaata cggaagtaaa gatcatcaat
     8521 atgagtccta tcagactcat cttttttatc gtgtgaggca tgaaaccatc cttcatttca
     8581 gcgcgtgtat aacacgtgcc cactgaactg cactgcgcaa cattgagcag gcagcgtaaa
     8641 aaccaataca gaattgatac ctggatgata tcaaatatcc agtcttaaat tgtggccttt
     8701 aacggaaaaa tgaaaacata tgctacagaa tggcgcttat cacgccattc ttcgctgtta
     8761 attcagtaat ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg
     8821 tctccgcgcc cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt
     8881 caggcatacc gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac
     8941 cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg
     9001 tcaattcatg gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag
     9061 cgacccgatg atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt
     9121 acacaaaggt cggtttttct ttgccaatat ttggctctac gccaaaggta aacagcaatt
     9181 gtagcagcgt gtcgcggtct tcttcggtat cagcaacatt gctcaaatcc agtttcgctg
     9241 cgacttcccg cagttgcgtt ttgtcggcag agagcgggtc aatttccaga taacgcaaga
     9301 aagcttgttg ataagaaagg ctttctgctg ccgggcagtc cagcacctgt tgtaagagat
     9361 cgtccacctc gttcatcaac cggtacatat catagtgcgg tcgataccac tccagcatag
     9421 tgaactcagg gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga
     9481 ataccggccc acaaccggca accagcaggc gtttcatatg gtattccggg ctggtcatta
     9541 accagagatt catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca
     9601 aatgaatatc ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac
     9661 gatcggcaaa gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa
     9721 tggatgcgct cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca
     9781 agggcacgaa gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aatcaaacag
     9841 cggtgggcag tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt
     9901 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
     9961 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
    10021 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
    10081 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
    10141 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
    10201 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
    10261 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
    10321 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
    10381 ggggtgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
    10441 gaaaatcgag cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
    10501 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
    10561 tattctggtt gatgatggtc atgttcgcgg cgtggtagca atgaacatga tggaaggcac
    10621 gctggtgcag atccgtgcta acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta
    10681 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
    10741 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
    10801 cggtatcctg atgaccgaag gctgccgcgg tgaaggcggt attctggtca acaaaaatgg
    10861 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
    10921 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
    10981 taaaggcaac accatctcca cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct
    11041 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
    11101 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg
    11161 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
    11221 atgttcctct gttggtctgc acggtgcaaa ccgtctgggt tctaactccc tggcggaact
    11281 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
    11341 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
    11401 ggttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
    11461 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
    11521 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa
    11581 caccgacctg ctctacacca ttgaactggg ccacggtctg aacgttgctg aatgtatggc
    11641 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
    11701 caccgagcgt gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg
    11761 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt
    11821 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc
    11881 gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata
    11941 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
    12001 cgctgggcta catcaaagac aacctggcac cggacctgag ttaccgctgg tcctgccgta
    12061 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
    12121 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
    12181 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
    12241 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
    12301 tggcaaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
    12361 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
    12421 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
    12481 acggcgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
    12541 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
    12601 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
    12661 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
    12721 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
    12781 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc
    12841 attaacctga tcactctggc ggcagctctg ctgcacacca aaacctggtt tgaactggca
    12901 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa
    12961 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
    13021 taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg
    13081 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
    13141 gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct
    13201 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
    13261 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc
    13321 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtat
    13381 cgttacaatc taacgtatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
    13441 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
    13501 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
    13561 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
    13621 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
    13681 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
    13741 cgaggggaaa tcaagttaag cgatcccaca acaaaatact ggcctgaact taccgctaaa
    13801 cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg cctgccattg
    13861 caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
    13921 cctgcatggg cgccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
    13981 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac acgtgtcttc
    14041 cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga aaagaattac
    14101 gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt agatgctgaa
    14161 acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag caatatgaat
    14221 ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca atctcgctac
    14281 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtaaat
    14341 cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcaca ccccgtaaaa
    14401 gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac aggggcaacc
    14461 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat agtgatgctg
    14521 gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat tctcaacgct
    14581 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
    14641 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctc
//
LOCUS       DAZFDP010000652         8463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.183, whole genome shotgun sequence.
ACCESSION   DAZFDP010000652 DAZFDP010000000
VERSION     DAZFDP010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.183"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1140..2126
                     /gene="mobV"
                     /locus_tag="ACHJ1G_005981"
     CDS             1140..2126
                     /gene="mobV"
                     /locus_tag="ACHJ1G_005981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFO4767561.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHL
                     AARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWL
                     ADKYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAER
                     VADLGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELNPQKVKGVSLAEKVFG
                     AVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPF
                     KGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
     gene            complement(2361..2582)
                     /locus_tag="ACHJ1G_005982"
     CDS             complement(2361..2582)
                     /locus_tag="ACHJ1G_005982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767562.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(2573..2776)
                     /locus_tag="ACHJ1G_005983"
     CDS             complement(2573..2776)
                     /locus_tag="ACHJ1G_005983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4767563.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
     gene            3113..3928
                     /gene="sul2"
                     /locus_tag="ACHJ1G_005984"
     CDS             3113..3928
                     /gene="sul2"
                     /locus_tag="ACHJ1G_005984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO4767564.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3989..4792
                     /locus_tag="ACHJ1G_005985"
                     /note="aph(3'')-Ib"
     CDS             3989..4792
                     /locus_tag="ACHJ1G_005985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO4767565.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4792..5628
                     /locus_tag="ACHJ1G_005986"
     CDS             4792..5628
                     /locus_tag="ACHJ1G_005986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO4767566.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5741..5923)
                     /locus_tag="ACHJ1G_005987"
                     /pseudo
     CDS             complement(5741..5923)
                     /locus_tag="ACHJ1G_005987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(5962..>8463)
                     /locus_tag="ACHJ1G_005988"
     CDS             complement(5962..>8463)
                     /locus_tag="ACHJ1G_005988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO4767567.1"
                     /translation="RQKAASSLTLQQCHKLALIPSAEQRSQLEMLLGPTDCSRLSLLE
                     SLKKGPVTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIA
                     RMSPQKRMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDK
                     SALALASACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMV
                     EQYGRVRRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSW
                     KRLVINKEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANR
                     IKVYRSLGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLA
                     SLDEPDSLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSI
                     SAVLMAEACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPL
                     AQIWGGGEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIP
                     GTLRDSIFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASV
                     FWRMDHDADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSE
                     RPSGLTQAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGE
                     IRKRYTDGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCH
                     GHINMLGHYSFTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT         1848 a         2380 c         2346 g         1889 t
ORIGIN      
        1 tcactaacgt acgttttcgt tccattggac ttcagacccc ctccctgacg cgccgccatc
       61 cctttgaata tacctgtgtg aaccccttgt tctttcgcgc ctcctcgcgc tcctgagcct
      121 ccttctcgtc tgctgctgcc agctcccttt gcctgattcg tctctgtgtc tctgtcgcta
      181 cggtgacgct ttgcattgtt gacctcctaa ccggaaacag ggcgtccgct taaccggacg
      241 tttaattcct accataggaa aattacttcc caaaaccgtt tcccaagtca agcaatttgt
      301 ttcctatgat aggaagtaag cggaaacaag ctgcttccgg ttgtccggaa ccctacgttt
      361 cctatcacaa aatttaaaaa ctatataaat caatcattta gtatttttcc ccttctcctc
      421 taagggactg ccgtcccgcc ggcgcttcgc ttggcaccct gctgacggtc gctgcgctcc
      481 cttggagtta atgcctcgcc agaggcgaga gggtttgttt ccgctgcgct aggcccaaag
      541 gggctttgcc cctctggact ccccacactc gccgccgtga ggctcggggg ctggggcctg
      601 cggtcgctgc gctcccttcg ttcccaaccc cagagccaga ggctgggtcg ttccggcagc
      661 gtcaaggggc tttcgcggca tatcctcagg cgctgcgcgc cttgcggtat tccacgaccc
      721 cttgaccccg cctccactcg gaacggaatc aggtcaaggg cgggagcgga agccgcagcc
      781 ctggagcgaa gcggaaggga tcgggtgagc acccggcgaa gcgaaccctt gacgtgatgg
      841 agtgaccccc acgctcaggc aagggggcag gtagagcaga gcgaagcggc gcggccacct
      901 gcacccctgc cacgtggcga acgcggggtt tggggcagcg ccccaaggtg ttcgcaacga
      961 ccgaagggag gcgcagggcg aagcccgtag agcaaggccg caggccgctg caaccgcgta
     1021 gcggtcacgt actttacgga gtaaagtcta gtgagtttac acttcaaaga agtggcccgc
     1081 cggaggcaca ttcgtacctc gacgggctgg cagcaatctg acagtgcgga gggggacgga
     1141 tggcgtttgc gatcatgagg gcgaagaagc tcaacagcat gggcacggtg gcggctgcgt
     1201 tgcagcactg ctaccgcgac cgcgagacgc cgaacgccga tcaggagcgc acaccggaca
     1261 acgatcacct ggcggccaga tcgaccgatg aggcgatggg caagctcagg gagcggttgc
     1321 cggagaagcg gcgcaaggat gcggtgctgg cggtcgaata cgtgatgagc gcctcgccgg
     1381 agtggtggca gaccgcaagc gccgaccagc agcgggagtt cttcaagcga tcgaccgagt
     1441 ggctggccga caagtacggc gctgagaacg tggtcacggc cacgatccac cgggatgagc
     1501 tgaccccgca cctgtcggcg ttcgtggtgc ctcggacggc tgacgggcgc ctgagtgcca
     1561 aggagttcat tggcaaccgg acgaagatga gccaggacca gaccagctac gccgaacggg
     1621 tggccgatct ggggcttgaa cggggcatcg agggcagcag ggccacacac cagcgagtta
     1681 aaacccacta tggggcgatc cagcaggcag gccgagacgt gccacacctg acgcctgacg
     1741 agctgaaccc ccagaaggtc aagggggtgt cactggccga gaaggtgttc ggagccgtgg
     1801 agacggtcga aggggtggcg caaaggttga acgccaaaat aatggggtct gtgcagccga
     1861 tggccgagaa agccgctgta agcgcccaga acgagcgaag agccaaggag ctaagggaaa
     1921 ccctagccca gcagcaaaaa cgcttacagg cccttcagga gccgtttaag gggctatcca
     1981 aggatcaggt agcgggactg atccggcagg cggtgaaatt gcggcaggag aacgagcagg
     2041 agaagcagga gcgcgcccag cagatcaaag agcgattcaa ggccaagcgg gaacgggagc
     2101 gcagcgaccg cagcaggggc cgttaaccct tttcaaccaa ctttaaccaa ccttgaacaa
     2161 ccttaaacca ttgtttaacc aacattaaac cactttcaac caaccctaaa ccactttcaa
     2221 ccaaccctaa gcaaccgggg tgtaagggct ggcagtaatc gcgcggattt acaggcacgt
     2281 cggatatgtt tacaaaaact ccgtttttga cacatatccc gtgccaaagg gtgccccttt
     2341 gaaaccctcc agcgccccgc tcaatcgcgg ggcagggtga cgcggtgggg taggtcggga
     2401 ctggggccgt cctcgtccag gaacgccact tcggcgcgtt ccagggtgca gccatcgagg
     2461 ttcaattctt ccatgtcctt gagggtgaaa acccccgcct cctcgcggct cttggtgtac
     2521 ccgtagccct tgggtttcca gtactccaga cctgtcttga tgtaatacat gctcatggcc
     2581 atacctcgtc cttgagtttc gatataacgg aatccagcgc cgttctggtg gtgccgtcca
     2641 gcgtgtctat acccctgtcg taagcctcca aaacagcggc catttcagcg gctgtgatgc
     2701 tgatagtggc cgtggtgtcc cttacgatat gggagtaatc aggcttgccg aaaagggctt
     2761 tgagtggatc tgtcatggct gatctccaaa catatcaagc gtgtctgtat cccactctat
     2821 tgtaaacaag acatttttat cttttatatt caatggctta ttttcctgct aattggtaat
     2881 accatgaaaa ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga
     2941 gcgctgccgc acagctccat aggccgcttt cctggctttg cttccagatg tatgctcttc
     3001 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     3061 ccgagcatcc gtatgatact catgctcgat tattattatt atagaagccc ccatgaataa
     3121 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     3181 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     3241 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     3301 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     3361 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     3421 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     3481 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc
     3541 tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg
     3601 tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc
     3661 tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga
     3721 tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg
     3781 tccgggggat gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc
     3841 tgacttcatc cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc
     3901 gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg
     3961 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
     4021 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
     4081 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
     4141 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg
     4201 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
     4261 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
     4321 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
     4381 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
     4441 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
     4501 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
     4561 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
     4621 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
     4681 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
     4741 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
     4801 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
     4861 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
     4921 aaacctataa aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     4981 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     5041 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     5101 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     5161 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     5221 ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     5281 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     5341 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     5401 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     5461 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     5521 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     5581 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     5641 ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc caaagcctta
     5701 gcgtaggatt ccgccccttc ccgcaaacga ccccaaacag gaaacgcagc tgaaacggga
     5761 agctcaacac ccactgacgc atgggttgtt caggcagtac ttcatcaacc agcaaggcgg
     5821 cactttcggc catccgccgc gccccacagc tcgggcagaa accgcgacgc ttacagctga
     5881 aagcgaccag gggctcggcg tggcaagact cgcagcgaac ccgtagaagg ggtctgacgc
     5941 tcagtggaac gaaaactcac gttaagcaac gttttctgcc tctgacgcct cttttaatgg
     6001 tctcagatgt cctttggtca ccagttctgc cagcgtgaag gaataatggc cgagcatatt
     6061 gatatgtccg tggcaaagcg gggagaggcg tgcgatatct tcatcattca gtgtttcacc
     6121 ctgcgcccgg agatgatcca gggctgcctg catataaata gtgttccata acacgacggc
     6181 gttagtgacc agccccagtg tgcccagttg atcttcctga ccgtcggtat atcgttttct
     6241 tatctcacct ttttgaccgt gacagatggc tctggcaacg gcatggcgac tttctccccg
     6301 attaagctgg gtcagaatgc gccggcggta atcttcatca tcaatataat taagcagata
     6361 cagcgttttg ttgatgcgcc ccacttcaat gattgcctga gtcagtccgg aaggacgttc
     6421 acttttcagc aatgaacgga ccagcactga aacctgtact ttgcccagct tcagggagcc
     6481 agcggtccgg atcatttcgt cccactgaag gactattttt cggggatctg attgccctct
     6541 ggcaatatca ttcagcacgc catagtcggc atcatggtcc attcgccaga aaaccgaagc
     6601 accggcatca gccaggcgtg gagaaaactg gtatcccagc agccagaaaa ggccaaagac
     6661 aagttcgctg gcacctgctg tatcggtcat aatttcggtt ggattcagcc cggtctcctg
     6721 ttccagaaga ccttccagca caaagataga gtccctcagc gtccccggta taacgatgcc
     6781 atgaaagccg gaatactgat cggacacaaa gttgtaccag gtgatccctc tgttattacc
     6841 aaagtatttg cggttcggtc cggcattgat tgttctgact ggcgtaacaa agcgcattcc
     6901 atctgcagat gccacttctc ctccacccca tatctgtgcc agtggcagcg ttgcctgaaa
     6961 atcaaccagt ctggcattag cgctggtgat agtttcagcc cgcagatagt tcgcttttgt
     7021 ccagttcagc cggtgtcggg tcagtgcagg aacatttgat ctgatcagtg gttccagacc
     7081 gatattgcag gcttcagcca tcagcacggc gctgatgctg acgggcagat catcaactct
     7141 ggcactggct tcactagcat ggaaaaactc atcagcaaat ccggtatggg cgttaatttc
     7201 gagcagcaac tccgttaaat ccaccggagg gagtagatca ctgatcattt tgctcagtcg
     7261 tttcagactg tccggctcat caagactggc gaggggagaa attgtcaacc ggggcttcgg
     7321 gccagaaaca tcgagttcga cagcctcatt ttcgcaaaga cgtgcagcaa cctgtctgta
     7381 acgactatca agctgatgac ccagagattt tattgcttcc tgcgggtctg tcgggtgccc
     7441 caaagaacga taaaccttaa tccggtttgc ctgccagtca gcaccctgta gtaatcttgc
     7501 acgaggatct ccccaccggt tactgccggt aacgtagaca tccctccgcc tcagactatc
     7561 ctgcagttta ctgagaaagc agagcgtgta tcccctgcgg gtgatatgtt tttccttgtt
     7621 aatcaccagc cgtttccatg accgactgat aatttccgtt ggtgcgtcgt caaaaaactg
     7681 ccgccgtgag ctgaactccc ggctgaggta gtcacaggca ttcagagtgg taaccccggc
     7741 aggtgcggat gaaaatttaa cggtattcag cagatggggc aggaaacgac gaacgcgccc
     7801 gtactgctcc accatttctt catgaaaatt atcgtctgag ggccgggcaa tttcacggac
     7861 aagcgtgatg atttcagcca gcttttgcct tgggatgtag ctgaacacct cagcacgaat
     7921 cgattcgtcc ggtgtttctt ctttcagcag gtacgaacat gcgctggcga gcgccaatgc
     7981 agatttatcc agatccttca gcgagcggag ccgttttttc tgcccaatct ttctggcgtc
     8041 acggatgata acggccagca tggcgtccag aacgtccaat gcatcatcca gcgccagcgt
     8101 ttcccatgca aggacaaagg caaccagaac cgccatcctt ttctgcggtg acatcctggc
     8161 aatattgaac accgaagtca taccagcata acgtgcgaga tttttcaggc gcacagccgg
     8221 gagtgtactc aggttttcag catgcaggcc aaaatcgttc agagttttcc agcgttcaat
     8281 tgcttcatta aacgccggac cactgatggt cacagggccc tttttcagtg attccagtaa
     8341 agacaggcgg ctgcaatcag ttggccccag cagcatctcc agctgtgaac gctgttcggc
     8401 tgacggtatc agtgccagtt tgtggcactg ttgcaaagtt agcgatgagg cagccttttg
     8461 tct
//
LOCUS       DAZFDP010000653         1737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.388, whole genome shotgun sequence.
ACCESSION   DAZFDP010000653 DAZFDP010000000
VERSION     DAZFDP010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.388"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHJ1G_005989"
     CDS             263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHJ1G_005989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO4767568.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHJ1G_005990"
     CDS             1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHJ1G_005990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO4767569.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          462 a          304 c          428 g          543 t
ORIGIN      
        1 gagtttgcca gcctccccca gtggctggct tttttatgtc cgtaacatcc tgtgtatcaa
       61 taaatgttgt tatctacgta cgtcaagtag tcgcatgaga tctgaccaga tatgttaagg
      121 ttgcagctct ctttgaatat gattatcatt ttcattacgt tattgttacg tttatccggt
      181 gcgccgtaaa acgccgtcct tcagggcgtg gaggatgtca agaatatagt tatcgtatgg
      241 tgctcaagga gtattgtgta atatgaaaat aattattttt agagtgctaa cttttttctt
      301 tgttatcttt tcagttaatg tggtggcgaa ggaatttacc ttagacttct cgactgcaaa
      361 gacgtatgta gattcgctga atgtcattcg ctctgcaata ggtactccat tacagactat
      421 ttcatcagga ggtacgtctt tactgatgat tgatagtggc tcaggggata atttgtttgc
      481 agttgatgtc agagggatag atccagagga agggcggttt aataatctac ggcttattgt
      541 tgaacgaaat aatttatatg tgacaggatt tgttaacagg acaaataatg ttttttatcg
      601 ctttgctgat ttttcacatg ttacctttcc aggtacaaca gcggttacat tgtctggtga
      661 cagtagctat accacgttac agcgtgttgc agggatcagt cgtacgggga tgcagataaa
      721 tcgccattcg ttgactactt cttatctgga tttaatgtcg catagtggaa cctcactgac
      781 gcagtctgtg gcaagagcga tgttacggtt tgttactgtg acagctgaag ctttacgttt
      841 tcggcaaata cagaggggat ttcgtacaac actggatgat ctcagtgggc gttcttatgt
      901 aatgactgct gaagatgttg atcttacatt gaactgggga aggttgagta gcgtcctgcc
      961 tgactatcat ggacaagact ctgttcgtgt aggaagaatt tcttttggaa gcattaatgc
     1021 aattctggga agcgtggcat taatactgaa ttgtcatcat catgcatcgc gagttgccag
     1081 aatggcatct gatgagtttc cttctatgtg tccggcagat ggaagagtcc gtgggattac
     1141 gcacaataaa atattgtggg attcatccac tctgggggca attctgatgc gcagaactat
     1201 tagcagttga gggggtaaaa tgaaaaaaac attattaata gctgcatcgc tttcattttt
     1261 ttcagcaagt gcgctggcga cgcctgattg tgtaactgga aaggtggagt atacaaaata
     1321 taatgatgac gataccttta cagttaaagt gggtgataaa gaattattta ccaacagatg
     1381 gaatcttcag tctcttcttc tcagtgcgca aattacgggg atgactgtaa ccattaaaac
     1441 taatgcctgt cataatggag ggggattcag cgaagttatt tttcgttgac tcagaatagc
     1501 tcagtgaaaa tagcaggcgg agattcataa atgttaaata catctcaatt cagtcagttg
     1561 ttgccggtct gataatagat gtgttagaaa atttctgcat ggtgaatccc cctgtgcgga
     1621 ggggcgactg gtgaacggta tgatctcttt gatgatcgta agcgagaata cgcgggtttg
     1681 gtggcaccag gccgaactca ccgggaggca cccggcatca tgctgtatac agagatt
//
LOCUS       DAZFDP010000654         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.439, whole genome shotgun sequence.
ACCESSION   DAZFDP010000654 DAZFDP010000000
VERSION     DAZFDP010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.439"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..593
                     /gene="dfrA8"
                     /locus_tag="ACHJ1G_005991"
     CDS             84..593
                     /gene="dfrA8"
                     /locus_tag="ACHJ1G_005991"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HFO4767570.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            590..1246
                     /locus_tag="ACHJ1G_005992"
     CDS             590..1246
                     /locus_tag="ACHJ1G_005992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO4767571.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLAGHYHNHKKIGTVANSRW
                     "
BASE COUNT          309 a          332 c          322 g          295 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cgacgcaata atcaccaatt
       61 cctcttttga taggtaaaca acaatgatcg agcttcatgc cattttagct gccaccgcca
      121 atggttgcat tgggaaggac aacgcacttc cctggccacc actaaaaggc gatctggcca
      181 gattcaaaaa attgaccatg gggaaggtgg tcattatggg gcgcaagacc tatgagagct
      241 tgcccgtcaa attagaaggt cgcacctgca tcgttatgac gcgccaagcg ctggagcttc
      301 cgggtgttcg tgacgctaac ggcgctatct tcgtgaacaa cgtcagcgac gccatgcggt
      361 tcgctcaaga agagagcgtg ggcgatgtgg cctacgtcat tggtggcgct gagatattca
      421 agcgacttgc cttgatgatc acgcagattg aattgacctt tgttaagcga ctgtacgaag
      481 gcgacaccta cgttgatctg gccgaaatgg tcaaagacta cgagcagaat ggcatggaag
      541 aacatgacct tcacacttac ttcacttacc gtaaaaagga gcttacagaa tgaccttcgt
      601 tgccatttca gatacgcacc tgcacaactg gtcgcagttc gcgataccaa cagagtcggg
      661 cattaactcc aggctgttgc agattcttaa agccattgaa gaagcggctt gcgccgccga
      721 ttaccacgcc ccagcgggcg tggttccaac cgtctatcac ggcggcgatc tcttccatgt
      781 gcgaggttct ttgacccctt ctgtgctaaa cgcggtgctc gactttttta agaccatcca
      841 ccgtgactac ggcgttcgct ttcgcatgat cgccggcaat cacgatctgg aaaccaaaga
      901 ctcatgtcct atgggcaatg cagctgcggc gcttaattcg ttgccgtttg tggaggttgt
      961 atccgaaaaa acgctgttcg aggaccacaa agtcgctctg ctgccatggc gcgattccat
     1021 ggacgatctg cgtgcggatc ttgcgcacgt taaagacgcg atcggcgcta gtgttgcctc
     1081 taagtggaca gcaatcatcc acgcaccggt taacggggtt gttcttggca ttccagacca
     1141 tgggttcgac ggaaaagagc tcgcctccta tggcttcggg ttagtgctcg ccggtcacta
     1201 ccacaaccac aaaaaaatcg gcactgttgc aaatagtcgg tggtgataaa cttatcat
//
LOCUS       DAZFDP010000655         1163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F66861
            SAMN43251690-rid23064413.guided.451, whole genome shotgun sequence.
ACCESSION   DAZFDP010000655 DAZFDP010000000
VERSION     DAZFDP010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251690
            Sequence Read Archive: SRR30293368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350193.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350193.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:24:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,993
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 106 of 262
            Pseudo Genes (incomplete)         :: 152 of 262
            Pseudo Genes (internal stop)      :: 63 of 262
            Pseudo Genes (multiple problems)  :: 52 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251690-rid23064413.guided.451"
                     /serotype="O 26:H11"
                     /isolate="1810F66861"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            268..1128
                     /locus_tag="ACHJ1G_005993"
     CDS             268..1128
                     /locus_tag="ACHJ1G_005993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO4767572.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          315 a          257 c          278 g          313 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc cgctccacgg tttataaaat
       61 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      121 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctggtaaatg
      241 cttcaataat attgaaaaag gaagagtatg agtattcaac attttcgtgt cgcccttatt
      301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      481 gttctgctat gtggtgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
      541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      661 gctgccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      781 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
      841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1141 gtttactcat atatacttta gat
//
